LOCUS       DABSYT020000001       176204 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.001, whole genome shotgun sequence.
ACCESSION   DABSYT020000001 DABSYT020000000
VERSION     DABSYT020000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 176204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 176204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 176204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..176204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.001"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..138)
                     /locus_tag="ID725_RS00005"
                     /old_locus_tag="pgaptmp_000001"
     CDS             complement(<1..138)
                     /locus_tag="ID725_RS00005"
                     /old_locus_tag="pgaptmp_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HGV7848697.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     MF"
     gene            complement(214..831)
                     /gene="yncG"
                     /locus_tag="ID725_RS00010"
                     /old_locus_tag="pgaptmp_000002"
     CDS             complement(214..831)
                     /gene="yncG"
                     /locus_tag="ID725_RS00010"
                     /old_locus_tag="pgaptmp_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGV7848698.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEII"
     gene            1098..2597
                     /gene="ansP"
                     /locus_tag="ID725_RS00015"
                     /old_locus_tag="pgaptmp_000003"
     CDS             1098..2597
                     /gene="ansP"
                     /locus_tag="ID725_RS00015"
                     /old_locus_tag="pgaptmp_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGV7848699.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2712..3773)
                     /gene="yncE"
                     /locus_tag="ID725_RS00020"
                     /old_locus_tag="pgaptmp_000004"
     CDS             complement(2712..3773)
                     /gene="yncE"
                     /locus_tag="ID725_RS00020"
                     /old_locus_tag="pgaptmp_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGV7848700.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4015..6117
                     /gene="pqqU"
                     /locus_tag="ID725_RS00025"
                     /old_locus_tag="pgaptmp_000005"
     CDS             4015..6117
                     /gene="pqqU"
                     /locus_tag="ID725_RS00025"
                     /old_locus_tag="pgaptmp_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGV7848701.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            complement(6153..6818)
                     /gene="mcbR"
                     /locus_tag="ID725_RS00030"
                     /old_locus_tag="pgaptmp_000006"
     CDS             complement(6153..6818)
                     /gene="mcbR"
                     /locus_tag="ID725_RS00030"
                     /old_locus_tag="pgaptmp_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGV7848702.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(7016..8053)
                     /gene="curA"
                     /locus_tag="ID725_RS00035"
                     /old_locus_tag="pgaptmp_000007"
     CDS             complement(7016..8053)
                     /gene="curA"
                     /locus_tag="ID725_RS00035"
                     /old_locus_tag="pgaptmp_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGV7848703.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8234..8752
                     /gene="mddA"
                     /locus_tag="ID725_RS00040"
                     /old_locus_tag="pgaptmp_000008"
     CDS             8234..8752
                     /gene="mddA"
                     /locus_tag="ID725_RS00040"
                     /old_locus_tag="pgaptmp_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGV7848704.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8749..9198
                     /gene="ydcZ"
                     /locus_tag="ID725_RS00045"
                     /old_locus_tag="pgaptmp_000009"
     CDS             8749..9198
                     /gene="ydcZ"
                     /locus_tag="ID725_RS00045"
                     /old_locus_tag="pgaptmp_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGV7848705.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9199..9432)
                     /gene="ydcY"
                     /locus_tag="ID725_RS00050"
                     /old_locus_tag="pgaptmp_000010"
     CDS             complement(9199..9432)
                     /gene="ydcY"
                     /locus_tag="ID725_RS00050"
                     /old_locus_tag="pgaptmp_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGV7848706.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9518..9691)
                     /gene="ortT"
                     /locus_tag="ID725_RS00055"
                     /old_locus_tag="pgaptmp_000011"
     CDS             complement(9518..9691)
                     /gene="ortT"
                     /locus_tag="ID725_RS00055"
                     /old_locus_tag="pgaptmp_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGV7848707.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9886..9981
                     /gene="yncL"
                     /locus_tag="ID725_RS00060"
                     /old_locus_tag="pgaptmp_000012"
     CDS             9886..9981
                     /gene="yncL"
                     /locus_tag="ID725_RS00060"
                     /old_locus_tag="pgaptmp_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGV7848708.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10078..11502)
                     /gene="patD"
                     /locus_tag="ID725_RS00065"
                     /old_locus_tag="pgaptmp_000013"
     CDS             complement(10078..11502)
                     /gene="patD"
                     /locus_tag="ID725_RS00065"
                     /old_locus_tag="pgaptmp_000013"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGV7848709.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11524..12318)
                     /gene="ydcV"
                     /locus_tag="ID725_RS00070"
                     /old_locus_tag="pgaptmp_000014"
     CDS             complement(11524..12318)
                     /gene="ydcV"
                     /locus_tag="ID725_RS00070"
                     /old_locus_tag="pgaptmp_000014"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7848710.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLESGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12308..13249)
                     /gene="ydcU"
                     /locus_tag="ID725_RS00075"
                     /old_locus_tag="pgaptmp_000015"
     CDS             complement(12308..13249)
                     /gene="ydcU"
                     /locus_tag="ID725_RS00075"
                     /old_locus_tag="pgaptmp_000015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7848711.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(13250..14263)
                     /gene="ydcT"
                     /locus_tag="ID725_RS00080"
                     /old_locus_tag="pgaptmp_000016"
     CDS             complement(13250..14263)
                     /gene="ydcT"
                     /locus_tag="ID725_RS00080"
                     /old_locus_tag="pgaptmp_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGV7848712.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14281..15426)
                     /gene="ydcS"
                     /locus_tag="ID725_RS00085"
                     /old_locus_tag="pgaptmp_000017"
     CDS             complement(14281..15426)
                     /gene="ydcS"
                     /locus_tag="ID725_RS00085"
                     /old_locus_tag="pgaptmp_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGV7848713.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15671..17077)
                     /gene="ydcR"
                     /locus_tag="ID725_RS00090"
                     /old_locus_tag="pgaptmp_000018"
     CDS             complement(15671..17077)
                     /gene="ydcR"
                     /locus_tag="ID725_RS00090"
                     /old_locus_tag="pgaptmp_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGV7848714.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(17156..17572)
                     /gene="hicB"
                     /locus_tag="ID725_RS00095"
                     /old_locus_tag="pgaptmp_000019"
     CDS             complement(17156..17572)
                     /gene="hicB"
                     /locus_tag="ID725_RS00095"
                     /old_locus_tag="pgaptmp_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGV7848715.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17618..17794)
                     /gene="hicA"
                     /locus_tag="ID725_RS00100"
                     /old_locus_tag="pgaptmp_000020"
     CDS             complement(17618..17794)
                     /gene="hicA"
                     /locus_tag="ID725_RS00100"
                     /old_locus_tag="pgaptmp_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HGV7848716.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            18016..18246
                     /gene="yncJ"
                     /locus_tag="ID725_RS00105"
                     /old_locus_tag="pgaptmp_000021"
     CDS             18016..18246
                     /gene="yncJ"
                     /locus_tag="ID725_RS00105"
                     /old_locus_tag="pgaptmp_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGV7848717.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(18338..20299)
                     /gene="rlhA"
                     /locus_tag="ID725_RS00110"
                     /old_locus_tag="pgaptmp_000022"
     CDS             complement(18338..20299)
                     /gene="rlhA"
                     /locus_tag="ID725_RS00110"
                     /old_locus_tag="pgaptmp_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGV7848718.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20372..20908)
                     /gene="sutR"
                     /locus_tag="ID725_RS00115"
                     /old_locus_tag="pgaptmp_000023"
     CDS             complement(20372..20908)
                     /gene="sutR"
                     /locus_tag="ID725_RS00115"
                     /old_locus_tag="pgaptmp_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGV7848719.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            21000..22173
                     /gene="ydcO"
                     /locus_tag="ID725_RS00120"
                     /old_locus_tag="pgaptmp_000024"
                     /pseudo
     CDS             21000..22173
                     /gene="ydcO"
                     /locus_tag="ID725_RS00120"
                     /old_locus_tag="pgaptmp_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
     gene            complement(22208..22876)
                     /gene="ydcL"
                     /locus_tag="ID725_RS00125"
                     /old_locus_tag="pgaptmp_000025"
     CDS             complement(22208..22876)
                     /gene="ydcL"
                     /locus_tag="ID725_RS00125"
                     /old_locus_tag="pgaptmp_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGV7848720.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(23180..23773)
                     /gene="tehB"
                     /locus_tag="ID725_RS00130"
                     /old_locus_tag="pgaptmp_000026"
     CDS             complement(23180..23773)
                     /gene="tehB"
                     /locus_tag="ID725_RS00130"
                     /old_locus_tag="pgaptmp_000026"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGV7848721.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(23770..24762)
                     /gene="tehA"
                     /locus_tag="ID725_RS00135"
                     /old_locus_tag="pgaptmp_000027"
     CDS             complement(23770..24762)
                     /gene="tehA"
                     /locus_tag="ID725_RS00135"
                     /old_locus_tag="pgaptmp_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGV7848722.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            24886..25866
                     /gene="ydcK"
                     /locus_tag="ID725_RS00140"
                     /old_locus_tag="pgaptmp_000028"
     CDS             24886..25866
                     /gene="ydcK"
                     /locus_tag="ID725_RS00140"
                     /old_locus_tag="pgaptmp_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGV7848723.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(25861..26397)
                     /gene="rimL"
                     /locus_tag="ID725_RS00145"
                     /old_locus_tag="pgaptmp_000029"
                     /pseudo
     CDS             complement(25861..26397)
                     /gene="rimL"
                     /locus_tag="ID725_RS00145"
                     /old_locus_tag="pgaptmp_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(26460..26684)
                     /gene="ydcH"
                     /locus_tag="ID725_RS00150"
                     /old_locus_tag="pgaptmp_000030"
     CDS             complement(26460..26684)
                     /gene="ydcH"
                     /locus_tag="ID725_RS00150"
                     /old_locus_tag="pgaptmp_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGV7848724.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(26824..28479)
                     /gene="opgD"
                     /locus_tag="ID725_RS00155"
                     /old_locus_tag="pgaptmp_000031"
                     /gene_synonym="mdoD"
     CDS             complement(26824..28479)
                     /gene="opgD"
                     /locus_tag="ID725_RS00155"
                     /old_locus_tag="pgaptmp_000031"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGV7848725.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(28704..30047)
                     /gene="ydcJ"
                     /locus_tag="ID725_RS00160"
                     /old_locus_tag="pgaptmp_000032"
     CDS             complement(28704..30047)
                     /gene="ydcJ"
                     /locus_tag="ID725_RS00160"
                     /old_locus_tag="pgaptmp_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGV7848726.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            30264..31187
                     /gene="ydcI"
                     /locus_tag="ID725_RS00165"
                     /old_locus_tag="pgaptmp_000033"
     CDS             30264..31187
                     /gene="ydcI"
                     /locus_tag="ID725_RS00165"
                     /old_locus_tag="pgaptmp_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGV7848727.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(31225..32865)
                     /gene="trg"
                     /locus_tag="ID725_RS00170"
                     /old_locus_tag="pgaptmp_000034"
     CDS             complement(31225..32865)
                     /gene="trg"
                     /locus_tag="ID725_RS00170"
                     /old_locus_tag="pgaptmp_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGV7848728.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            33264..33413
                     /gene="hokB"
                     /locus_tag="ID725_RS00175"
                     /old_locus_tag="pgaptmp_000035"
     CDS             33264..33413
                     /gene="hokB"
                     /locus_tag="ID725_RS00175"
                     /old_locus_tag="pgaptmp_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGV7848729.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(33391..33459)
                     /gene="yncP"
                     /locus_tag="ID725_RS00180"
                     /old_locus_tag="pgaptmp_000036"
     CDS             complement(33391..33459)
                     /gene="yncP"
                     /locus_tag="ID725_RS00180"
                     /old_locus_tag="pgaptmp_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HGV7848730.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(33485..33658)
                     /locus_tag="ID725_RS00185"
                     /old_locus_tag="pgaptmp_000037"
     CDS             complement(33485..33658)
                     /locus_tag="ID725_RS00185"
                     /old_locus_tag="pgaptmp_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7848731.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(33903..34433)
                     /gene="cybB"
                     /locus_tag="ID725_RS00190"
                     /old_locus_tag="pgaptmp_000038"
     CDS             complement(33903..34433)
                     /gene="cybB"
                     /locus_tag="ID725_RS00190"
                     /old_locus_tag="pgaptmp_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGV7848732.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            34622..35623
                     /gene="gap"
                     /locus_tag="ID725_RS00195"
                     /old_locus_tag="pgaptmp_000039"
     CDS             34622..35623
                     /gene="gap"
                     /locus_tag="ID725_RS00195"
                     /old_locus_tag="pgaptmp_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGV7848733.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(35665..37104)
                     /gene="aldA"
                     /locus_tag="ID725_RS00200"
                     /old_locus_tag="pgaptmp_000040"
     CDS             complement(35665..37104)
                     /gene="aldA"
                     /locus_tag="ID725_RS00200"
                     /old_locus_tag="pgaptmp_000040"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGV7848734.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(37301..38101)
                     /gene="ydcF"
                     /locus_tag="ID725_RS00205"
                     /old_locus_tag="pgaptmp_000041"
     CDS             complement(37301..38101)
                     /gene="ydcF"
                     /locus_tag="ID725_RS00205"
                     /old_locus_tag="pgaptmp_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGV7848735.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(38217..38594)
                     /locus_tag="ID725_RS00210"
                     /old_locus_tag="pgaptmp_000042"
     CDS             complement(38217..38594)
                     /locus_tag="ID725_RS00210"
                     /old_locus_tag="pgaptmp_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7848736.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
     gene            complement(38719..39150)
                     /locus_tag="ID725_RS00215"
                     /old_locus_tag="pgaptmp_000043"
     CDS             complement(38719..39150)
                     /locus_tag="ID725_RS00215"
                     /old_locus_tag="pgaptmp_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7848737.1"
                     /translation="MNVRNVKFDNLSFYEEKIRWRMRRQISNAEAIFYENQEEHIRRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSMFGEHCHHIYLDDIKKKIQTELPENKTL
                     DFKRQLQFIILGDDKIRIWVPEGKLYFALMNVLQMFPMKNG"
     gene            complement(39137..39475)
                     /locus_tag="ID725_RS00220"
                     /old_locus_tag="pgaptmp_000044"
     CDS             complement(39137..39475)
                     /locus_tag="ID725_RS00220"
                     /old_locus_tag="pgaptmp_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7848738.1"
                     /translation="MKNVENIIWHDGVLVETRMECHHTEYNFILTAELYVDEFTAGRE
                     RKRFEFTGVEHLSQVMDIAELIDNQNAGNIKDATIVYKAKKYKISLHLFGGFLSFTFK
                     TMRVIDNECA"
     gene            complement(39480..39905)
                     /locus_tag="ID725_RS00225"
                     /old_locus_tag="pgaptmp_000045"
     CDS             complement(39480..39905)
                     /locus_tag="ID725_RS00225"
                     /old_locus_tag="pgaptmp_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7848739.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLPEENYRRALYIL
                     SIIYPIKLNVGDDEFKFIFYIMSQKKFLRQQTISDFVRSINVIEFTETQKSVLRELIK
                     KNNDIIITQCTFELDCLLTRVSASSNQFRNSNGYLPENS"
     gene            complement(40051..43953)
                     /gene="hrpA"
                     /locus_tag="ID725_RS00230"
                     /old_locus_tag="pgaptmp_000046"
     CDS             complement(40051..43953)
                     /gene="hrpA"
                     /locus_tag="ID725_RS00230"
                     /old_locus_tag="pgaptmp_000046"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGV7848740.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            44154..44759
                     /gene="azoR"
                     /locus_tag="ID725_RS00235"
                     /old_locus_tag="pgaptmp_000047"
     CDS             44154..44759
                     /gene="azoR"
                     /locus_tag="ID725_RS00235"
                     /old_locus_tag="pgaptmp_000047"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGV7848741.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(44810..46102)
                     /gene="ynbD"
                     /locus_tag="ID725_RS00240"
                     /old_locus_tag="pgaptmp_000048"
     CDS             complement(44810..46102)
                     /gene="ynbD"
                     /locus_tag="ID725_RS00240"
                     /old_locus_tag="pgaptmp_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGV7848742.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(46116..47873)
                     /gene="ynbC"
                     /locus_tag="ID725_RS00245"
                     /old_locus_tag="pgaptmp_000049"
     CDS             complement(46116..47873)
                     /gene="ynbC"
                     /locus_tag="ID725_RS00245"
                     /old_locus_tag="pgaptmp_000049"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGV7848743.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(47889..48785)
                     /gene="ynbB"
                     /locus_tag="ID725_RS00250"
                     /old_locus_tag="pgaptmp_000050"
     CDS             complement(47889..48785)
                     /gene="ynbB"
                     /locus_tag="ID725_RS00250"
                     /old_locus_tag="pgaptmp_000050"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGV7848744.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(48785..49390)
                     /gene="ynbA"
                     /locus_tag="ID725_RS00255"
                     /old_locus_tag="pgaptmp_000051"
     CDS             complement(48785..49390)
                     /gene="ynbA"
                     /locus_tag="ID725_RS00255"
                     /old_locus_tag="pgaptmp_000051"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGV7848745.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(49561..51867)
                     /gene="ydbD"
                     /locus_tag="ID725_RS00260"
                     /old_locus_tag="pgaptmp_000052"
     CDS             complement(49561..51867)
                     /gene="ydbD"
                     /locus_tag="ID725_RS00260"
                     /old_locus_tag="pgaptmp_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGV7848746.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIETFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(51931..52791)
                     /gene="pdxI"
                     /locus_tag="ID725_RS00265"
                     /old_locus_tag="pgaptmp_000053"
     CDS             complement(51931..52791)
                     /gene="pdxI"
                     /locus_tag="ID725_RS00265"
                     /old_locus_tag="pgaptmp_000053"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGV7848747.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(53011..55470)
                     /locus_tag="ID725_RS00270"
                     /old_locus_tag="pgaptmp_000054"
     CDS             complement(53011..55470)
                     /locus_tag="ID725_RS00270"
                     /old_locus_tag="pgaptmp_000054"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGV7848748.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(55690..58725)
                     /locus_tag="ID725_RS00275"
                     /old_locus_tag="pgaptmp_000055"
     CDS             complement(55690..58725)
                     /locus_tag="ID725_RS00275"
                     /old_locus_tag="pgaptmp_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HGV7848749.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVP
                     PDDGGDTPVKHNPVVYKNGVTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIV
                     KSWTVHDDSNTVVFDGVNTSGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAII
                     TDQGGNTALNGGTVMTVDGDNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVD
                     GGTAVIINGDNTKLNTAGDSTITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVG
                     DGATIKQEGDLYVSGGAHGIDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMG
                     DLNVGQAAAGENAIGIQIDGDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVG
                     DFSTGLDVSGNNNNMTLATYELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNA
                     QPYFFNPSTGVNISGDNNDVTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDD
                     NTFTLNGGVHFVGEKNVMDDGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFP
                     LGFENLIQLSHGAELEIGADATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVEL
                     KNIGFAAAWNKDSTVINNGSIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQ
                     HSVLNYPAEWNLSDGTSFNNKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAID
                     KSTADNEGQITLDTLWVDENDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATAT
                     NHENGVITVYNAGAGMAAYGNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGT
                     AINDQTGVININAENGQAFYNDGTGLIINYGTICTFGVC"
     gene            complement(59139..59462)
                     /locus_tag="ID725_RS00280"
                     /old_locus_tag="pgaptmp_000056"
     CDS             complement(59139..59462)
                     /locus_tag="ID725_RS00280"
                     /old_locus_tag="pgaptmp_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGV7848750.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(59470..59655)
                     /gene="ynbE"
                     /locus_tag="ID725_RS00285"
                     /old_locus_tag="pgaptmp_000057"
     CDS             complement(59470..59655)
                     /gene="ynbE"
                     /locus_tag="ID725_RS00285"
                     /old_locus_tag="pgaptmp_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGV7848751.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(59652..62291)
                     /gene="ydbH"
                     /locus_tag="ID725_RS00290"
                     /old_locus_tag="pgaptmp_000058"
     CDS             complement(59652..62291)
                     /gene="ydbH"
                     /locus_tag="ID725_RS00290"
                     /old_locus_tag="pgaptmp_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGV7848752.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            62499..63488
                     /gene="ldhA"
                     /locus_tag="ID725_RS00295"
                     /old_locus_tag="pgaptmp_000059"
     CDS             62499..63488
                     /gene="ldhA"
                     /locus_tag="ID725_RS00295"
                     /old_locus_tag="pgaptmp_000059"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGV7848753.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            63599..64021
                     /gene="hslJ"
                     /locus_tag="ID725_RS00300"
                     /old_locus_tag="pgaptmp_000060"
     CDS             63599..64021
                     /gene="hslJ"
                     /locus_tag="ID725_RS00300"
                     /old_locus_tag="pgaptmp_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGV7848754.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(64018..64284)
                     /gene="ydbJ"
                     /locus_tag="ID725_RS00305"
                     /old_locus_tag="pgaptmp_000061"
     CDS             complement(64018..64284)
                     /gene="ydbJ"
                     /locus_tag="ID725_RS00305"
                     /old_locus_tag="pgaptmp_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGV7848755.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            64558..68082
                     /gene="nifJ"
                     /locus_tag="ID725_RS00310"
                     /old_locus_tag="pgaptmp_000062"
     CDS             64558..68082
                     /gene="nifJ"
                     /locus_tag="ID725_RS00310"
                     /old_locus_tag="pgaptmp_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGV7848756.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            68448..69561
                     /gene="ompN"
                     /locus_tag="ID725_RS00315"
                     /old_locus_tag="pgaptmp_000063"
                     /pseudo
     CDS             68448..69561
                     /gene="ompN"
                     /locus_tag="ID725_RS00315"
                     /old_locus_tag="pgaptmp_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            69702..70136
                     /gene="uspF"
                     /locus_tag="ID725_RS00320"
                     /old_locus_tag="pgaptmp_000064"
     CDS             69702..70136
                     /gene="uspF"
                     /locus_tag="ID725_RS00320"
                     /old_locus_tag="pgaptmp_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGV7848757.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            70312..71247
                     /gene="ttcA"
                     /locus_tag="ID725_RS00325"
                     /old_locus_tag="pgaptmp_000065"
     CDS             70312..71247
                     /gene="ttcA"
                     /locus_tag="ID725_RS00325"
                     /old_locus_tag="pgaptmp_000065"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGV7848758.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(71376..72749)
                     /gene="dbpA"
                     /locus_tag="ID725_RS00330"
                     /old_locus_tag="pgaptmp_000066"
     CDS             complement(71376..72749)
                     /gene="dbpA"
                     /locus_tag="ID725_RS00330"
                     /old_locus_tag="pgaptmp_000066"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGV7848759.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(72782..72952)
                     /gene="ynaL"
                     /locus_tag="ID725_RS00335"
                     /old_locus_tag="pgaptmp_000067"
     CDS             complement(72782..72952)
                     /gene="ynaL"
                     /locus_tag="ID725_RS00335"
                     /old_locus_tag="pgaptmp_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGV7848760.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(73227..74210)
                     /gene="zntB"
                     /locus_tag="ID725_RS00340"
                     /old_locus_tag="pgaptmp_000068"
     CDS             complement(73227..74210)
                     /gene="zntB"
                     /locus_tag="ID725_RS00340"
                     /old_locus_tag="pgaptmp_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGV7848761.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(74224..74271)
                     /gene="ydaN"
                     /locus_tag="ID725_RS00345"
                     /old_locus_tag="pgaptmp_000069"
     CDS             complement(74224..74271)
                     /gene="ydaN"
                     /locus_tag="ID725_RS00345"
                     /old_locus_tag="pgaptmp_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGV7848762.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            74465..75697
                     /gene="dgcM"
                     /locus_tag="ID725_RS00350"
                     /old_locus_tag="pgaptmp_000070"
     CDS             74465..75697
                     /gene="dgcM"
                     /locus_tag="ID725_RS00350"
                     /old_locus_tag="pgaptmp_000070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGV7848763.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(75718..76281)
                     /gene="smrA"
                     /locus_tag="ID725_RS00355"
                     /old_locus_tag="pgaptmp_000071"
     CDS             complement(75718..76281)
                     /gene="smrA"
                     /locus_tag="ID725_RS00355"
                     /old_locus_tag="pgaptmp_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGV7848764.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(76611..77519)
                     /gene="abgR"
                     /locus_tag="ID725_RS00360"
                     /old_locus_tag="pgaptmp_000072"
     CDS             complement(76611..77519)
                     /gene="abgR"
                     /locus_tag="ID725_RS00360"
                     /old_locus_tag="pgaptmp_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7848765.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            77695..79005
                     /gene="abgA"
                     /locus_tag="ID725_RS00365"
                     /old_locus_tag="pgaptmp_000073"
     CDS             77695..79005
                     /gene="abgA"
                     /locus_tag="ID725_RS00365"
                     /old_locus_tag="pgaptmp_000073"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGV7848766.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            79005..80450
                     /gene="abgB"
                     /locus_tag="ID725_RS00370"
                     /old_locus_tag="pgaptmp_000074"
     CDS             79005..80450
                     /gene="abgB"
                     /locus_tag="ID725_RS00370"
                     /old_locus_tag="pgaptmp_000074"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGV7848767.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            80487..82013
                     /gene="abgT"
                     /locus_tag="ID725_RS00375"
                     /old_locus_tag="pgaptmp_000075"
     CDS             80487..82013
                     /gene="abgT"
                     /locus_tag="ID725_RS00375"
                     /old_locus_tag="pgaptmp_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGV7848768.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            82024..82539
                     /gene="ogt"
                     /locus_tag="ID725_RS00380"
                     /old_locus_tag="pgaptmp_000076"
     CDS             82024..82539
                     /gene="ogt"
                     /locus_tag="ID725_RS00380"
                     /old_locus_tag="pgaptmp_000076"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGV7848769.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            82734..83486
                     /gene="fnr"
                     /locus_tag="ID725_RS00385"
                     /old_locus_tag="pgaptmp_000077"
     CDS             82734..83486
                     /gene="fnr"
                     /locus_tag="ID725_RS00385"
                     /old_locus_tag="pgaptmp_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGV7848770.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDTLAQLAGHTRNVA"
     gene            83638..84588
                     /gene="uspE"
                     /locus_tag="ID725_RS00390"
                     /old_locus_tag="pgaptmp_000078"
     CDS             83638..84588
                     /gene="uspE"
                     /locus_tag="ID725_RS00390"
                     /old_locus_tag="pgaptmp_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGV7848771.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(84638..84895)
                     /gene="ynaJ"
                     /locus_tag="ID725_RS00395"
                     /old_locus_tag="pgaptmp_000079"
     CDS             complement(84638..84895)
                     /gene="ynaJ"
                     /locus_tag="ID725_RS00395"
                     /old_locus_tag="pgaptmp_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGV7848772.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            85139..86170
                     /gene="ynaI"
                     /locus_tag="ID725_RS00400"
                     /old_locus_tag="pgaptmp_000080"
     CDS             85139..86170
                     /gene="ynaI"
                     /locus_tag="ID725_RS00400"
                     /old_locus_tag="pgaptmp_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGV7848773.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(86221..87834)
                     /gene="mppA"
                     /locus_tag="ID725_RS00405"
                     /old_locus_tag="pgaptmp_000081"
     CDS             complement(86221..87834)
                     /gene="mppA"
                     /locus_tag="ID725_RS00405"
                     /old_locus_tag="pgaptmp_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGV7848774.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVCSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(88170..89069)
                     /gene="pgrR"
                     /locus_tag="ID725_RS00410"
                     /old_locus_tag="pgaptmp_000082"
     CDS             complement(88170..89069)
                     /gene="pgrR"
                     /locus_tag="ID725_RS00410"
                     /old_locus_tag="pgaptmp_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGV7848775.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            89207..90127
                     /locus_tag="ID725_RS00415"
                     /old_locus_tag="pgaptmp_000083"
     CDS             89207..90127
                     /locus_tag="ID725_RS00415"
                     /old_locus_tag="pgaptmp_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGV7848776.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            90127..91056
                     /locus_tag="ID725_RS00420"
                     /old_locus_tag="pgaptmp_000084"
     CDS             90127..91056
                     /locus_tag="ID725_RS00420"
                     /old_locus_tag="pgaptmp_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGV7848777.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHL"
     gene            91077..91715
                     /locus_tag="ID725_RS00425"
                     /old_locus_tag="pgaptmp_000085"
     CDS             91077..91715
                     /locus_tag="ID725_RS00425"
                     /old_locus_tag="pgaptmp_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGV7848778.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            92006..92734
                     /gene="mpaA"
                     /locus_tag="ID725_RS00430"
                     /old_locus_tag="pgaptmp_000086"
     CDS             92006..92734
                     /gene="mpaA"
                     /locus_tag="ID725_RS00430"
                     /old_locus_tag="pgaptmp_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGV7848779.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(92709..93674)
                     /gene="ycjG"
                     /locus_tag="ID725_RS00435"
                     /old_locus_tag="pgaptmp_000087"
     CDS             complement(92709..93674)
                     /gene="ycjG"
                     /locus_tag="ID725_RS00435"
                     /old_locus_tag="pgaptmp_000087"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGV7848780.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            93793..94299
                     /gene="tpx"
                     /locus_tag="ID725_RS00440"
                     /old_locus_tag="pgaptmp_000088"
     CDS             93793..94299
                     /gene="tpx"
                     /locus_tag="ID725_RS00440"
                     /old_locus_tag="pgaptmp_000088"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGV7848781.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(94343..95884)
                     /gene="tyrR"
                     /locus_tag="ID725_RS00445"
                     /old_locus_tag="pgaptmp_000089"
     CDS             complement(94343..95884)
                     /gene="tyrR"
                     /locus_tag="ID725_RS00445"
                     /old_locus_tag="pgaptmp_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGV7848782.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(96032..97093)
                     /gene="ycjF"
                     /locus_tag="ID725_RS00450"
                     /old_locus_tag="pgaptmp_000090"
     CDS             complement(96032..97093)
                     /gene="ycjF"
                     /locus_tag="ID725_RS00450"
                     /old_locus_tag="pgaptmp_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGV7848783.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(97090..98487)
                     /gene="ycjX"
                     /locus_tag="ID725_RS00455"
                     /old_locus_tag="pgaptmp_000091"
     CDS             complement(97090..98487)
                     /gene="ycjX"
                     /locus_tag="ID725_RS00455"
                     /old_locus_tag="pgaptmp_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGV7848784.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            98642..99640
                     /gene="ycjW"
                     /locus_tag="ID725_RS00460"
                     /old_locus_tag="pgaptmp_000092"
     CDS             98642..99640
                     /gene="ycjW"
                     /locus_tag="ID725_RS00460"
                     /old_locus_tag="pgaptmp_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGV7848785.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            complement(99751..100656)
                     /gene="ompG"
                     /locus_tag="ID725_RS00465"
                     /old_locus_tag="pgaptmp_000093"
     CDS             complement(99751..100656)
                     /gene="ompG"
                     /locus_tag="ID725_RS00465"
                     /old_locus_tag="pgaptmp_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGV7848786.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(100701..101783)
                     /locus_tag="ID725_RS00470"
                     /old_locus_tag="pgaptmp_000094"
     CDS             complement(100701..101783)
                     /locus_tag="ID725_RS00470"
                     /old_locus_tag="pgaptmp_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGV7848787.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(101797..102456)
                     /gene="pgmB"
                     /locus_tag="ID725_RS00475"
                     /old_locus_tag="pgaptmp_000095"
     CDS             complement(101797..102456)
                     /gene="pgmB"
                     /locus_tag="ID725_RS00475"
                     /old_locus_tag="pgaptmp_000095"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGV7848788.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(102453..104720)
                     /gene="ycjT"
                     /locus_tag="ID725_RS00480"
                     /old_locus_tag="pgaptmp_000096"
     CDS             complement(102453..104720)
                     /gene="ycjT"
                     /locus_tag="ID725_RS00480"
                     /old_locus_tag="pgaptmp_000096"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGV7848789.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(104717..105772)
                     /gene="ycjS"
                     /locus_tag="ID725_RS00485"
                     /old_locus_tag="pgaptmp_000097"
     CDS             complement(104717..105772)
                     /gene="ycjS"
                     /locus_tag="ID725_RS00485"
                     /old_locus_tag="pgaptmp_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGV7848790.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(105782..106570)
                     /gene="ycjR"
                     /locus_tag="ID725_RS00490"
                     /old_locus_tag="pgaptmp_000098"
     CDS             complement(105782..106570)
                     /gene="ycjR"
                     /locus_tag="ID725_RS00490"
                     /old_locus_tag="pgaptmp_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGV7848791.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(106588..107640)
                     /gene="ycjQ"
                     /locus_tag="ID725_RS00495"
                     /old_locus_tag="pgaptmp_000099"
     CDS             complement(106588..107640)
                     /gene="ycjQ"
                     /locus_tag="ID725_RS00495"
                     /old_locus_tag="pgaptmp_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGV7848792.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(107671..108513)
                     /gene="ycjP"
                     /locus_tag="ID725_RS00500"
                     /old_locus_tag="pgaptmp_000100"
     CDS             complement(107671..108513)
                     /gene="ycjP"
                     /locus_tag="ID725_RS00500"
                     /old_locus_tag="pgaptmp_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGV7848793.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(108500..109381)
                     /gene="ycjO"
                     /locus_tag="ID725_RS00505"
                     /old_locus_tag="pgaptmp_000101"
     CDS             complement(108500..109381)
                     /gene="ycjO"
                     /locus_tag="ID725_RS00505"
                     /old_locus_tag="pgaptmp_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGV7848794.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(109402..110694)
                     /gene="ycjN"
                     /locus_tag="ID725_RS00510"
                     /old_locus_tag="pgaptmp_000102"
                     /pseudo
     CDS             complement(109402..110694)
                     /gene="ycjN"
                     /locus_tag="ID725_RS00510"
                     /old_locus_tag="pgaptmp_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(110708..112386)
                     /gene="ycjM"
                     /locus_tag="ID725_RS00515"
                     /old_locus_tag="pgaptmp_000103"
                     /pseudo
     CDS             complement(110708..112386)
                     /gene="ycjM"
                     /locus_tag="ID725_RS00515"
                     /old_locus_tag="pgaptmp_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(112596..112910)
                     /gene="pspE"
                     /locus_tag="ID725_RS00520"
                     /old_locus_tag="pgaptmp_000104"
     CDS             complement(112596..112910)
                     /gene="pspE"
                     /locus_tag="ID725_RS00520"
                     /old_locus_tag="pgaptmp_000104"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGV7848795.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(112985..113206)
                     /gene="pspD"
                     /locus_tag="ID725_RS00525"
                     /old_locus_tag="pgaptmp_000105"
     CDS             complement(112985..113206)
                     /gene="pspD"
                     /locus_tag="ID725_RS00525"
                     /old_locus_tag="pgaptmp_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGV7848796.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(113215..113574)
                     /gene="pspC"
                     /locus_tag="ID725_RS00530"
                     /old_locus_tag="pgaptmp_000106"
     CDS             complement(113215..113574)
                     /gene="pspC"
                     /locus_tag="ID725_RS00530"
                     /old_locus_tag="pgaptmp_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGV7848797.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(113574..113798)
                     /gene="pspB"
                     /locus_tag="ID725_RS00535"
                     /old_locus_tag="pgaptmp_000107"
     CDS             complement(113574..113798)
                     /gene="pspB"
                     /locus_tag="ID725_RS00535"
                     /old_locus_tag="pgaptmp_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGV7848798.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(113852..114520)
                     /gene="pspA"
                     /locus_tag="ID725_RS00540"
                     /old_locus_tag="pgaptmp_000108"
     CDS             complement(113852..114520)
                     /gene="pspA"
                     /locus_tag="ID725_RS00540"
                     /old_locus_tag="pgaptmp_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGV7848799.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            114687..115664
                     /gene="pspF"
                     /locus_tag="ID725_RS00545"
                     /old_locus_tag="pgaptmp_000109"
     CDS             114687..115664
                     /gene="pspF"
                     /locus_tag="ID725_RS00545"
                     /old_locus_tag="pgaptmp_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGV7848800.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(115795..117060)
                     /gene="puuE"
                     /locus_tag="ID725_RS00550"
                     /old_locus_tag="pgaptmp_000110"
     CDS             complement(115795..117060)
                     /gene="puuE"
                     /locus_tag="ID725_RS00550"
                     /old_locus_tag="pgaptmp_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGV7848801.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(117098..118378)
                     /gene="puuB"
                     /locus_tag="ID725_RS00555"
                     /old_locus_tag="pgaptmp_000111"
     CDS             complement(117098..118378)
                     /gene="puuB"
                     /locus_tag="ID725_RS00555"
                     /old_locus_tag="pgaptmp_000111"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGV7848802.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(118380..119867)
                     /gene="puuC"
                     /locus_tag="ID725_RS00560"
                     /old_locus_tag="pgaptmp_000112"
     CDS             complement(118380..119867)
                     /gene="puuC"
                     /locus_tag="ID725_RS00560"
                     /old_locus_tag="pgaptmp_000112"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGV7848803.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(120017..120574)
                     /gene="puuR"
                     /locus_tag="ID725_RS00565"
                     /old_locus_tag="pgaptmp_000113"
     CDS             complement(120017..120574)
                     /gene="puuR"
                     /locus_tag="ID725_RS00565"
                     /old_locus_tag="pgaptmp_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGV7848804.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(120601..121365)
                     /gene="puuD"
                     /locus_tag="ID725_RS00570"
                     /old_locus_tag="pgaptmp_000114"
     CDS             complement(120601..121365)
                     /gene="puuD"
                     /locus_tag="ID725_RS00570"
                     /old_locus_tag="pgaptmp_000114"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGV7848805.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            121577..122995
                     /gene="puuA"
                     /locus_tag="ID725_RS00575"
                     /old_locus_tag="pgaptmp_000115"
     CDS             121577..122995
                     /gene="puuA"
                     /locus_tag="ID725_RS00575"
                     /old_locus_tag="pgaptmp_000115"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGV7848806.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            123144..123308
                     /locus_tag="ID725_RS00580"
                     /old_locus_tag="pgaptmp_000116"
     CDS             123144..123308
                     /locus_tag="ID725_RS00580"
                     /old_locus_tag="pgaptmp_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7848807.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            123298..124683
                     /gene="puuP"
                     /locus_tag="ID725_RS00585"
                     /old_locus_tag="pgaptmp_000117"
     CDS             123298..124683
                     /gene="puuP"
                     /locus_tag="ID725_RS00585"
                     /old_locus_tag="pgaptmp_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGV7848808.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            124817..125062
                     /gene="ymjA"
                     /locus_tag="ID725_RS00590"
                     /old_locus_tag="pgaptmp_000118"
     CDS             124817..125062
                     /gene="ymjA"
                     /locus_tag="ID725_RS00590"
                     /old_locus_tag="pgaptmp_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HGV7848809.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            125375..127018
                     /gene="sapA"
                     /locus_tag="ID725_RS00595"
                     /old_locus_tag="pgaptmp_000119"
     CDS             125375..127018
                     /gene="sapA"
                     /locus_tag="ID725_RS00595"
                     /old_locus_tag="pgaptmp_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGV7848810.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            127015..127980
                     /gene="sapB"
                     /locus_tag="ID725_RS00600"
                     /old_locus_tag="pgaptmp_000120"
     CDS             127015..127980
                     /gene="sapB"
                     /locus_tag="ID725_RS00600"
                     /old_locus_tag="pgaptmp_000120"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGV7848811.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            127967..128857
                     /gene="sapC"
                     /locus_tag="ID725_RS00605"
                     /old_locus_tag="pgaptmp_000121"
     CDS             127967..128857
                     /gene="sapC"
                     /locus_tag="ID725_RS00605"
                     /old_locus_tag="pgaptmp_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGV7848812.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            128857..129849
                     /gene="sapD"
                     /locus_tag="ID725_RS00610"
                     /old_locus_tag="pgaptmp_000122"
     CDS             128857..129849
                     /gene="sapD"
                     /locus_tag="ID725_RS00610"
                     /old_locus_tag="pgaptmp_000122"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGV7848813.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            129851..130657
                     /gene="sapF"
                     /locus_tag="ID725_RS00615"
                     /old_locus_tag="pgaptmp_000123"
     CDS             129851..130657
                     /gene="sapF"
                     /locus_tag="ID725_RS00615"
                     /old_locus_tag="pgaptmp_000123"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGV7848814.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(130709..131872)
                     /locus_tag="ID725_RS00620"
                     /old_locus_tag="pgaptmp_000124"
     CDS             complement(130709..131872)
                     /locus_tag="ID725_RS00620"
                     /old_locus_tag="pgaptmp_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HGV7848815.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(131881..133254)
                     /locus_tag="ID725_RS00625"
                     /old_locus_tag="pgaptmp_000125"
     CDS             complement(131881..133254)
                     /locus_tag="ID725_RS00625"
                     /old_locus_tag="pgaptmp_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HGV7848816.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(133258..136366)
                     /locus_tag="ID725_RS00630"
                     /old_locus_tag="pgaptmp_000126"
                     /pseudo
     CDS             complement(133258..136366)
                     /locus_tag="ID725_RS00630"
                     /old_locus_tag="pgaptmp_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(136366..137487)
                     /locus_tag="ID725_RS00635"
                     /old_locus_tag="pgaptmp_000127"
     CDS             complement(136366..137487)
                     /locus_tag="ID725_RS00635"
                     /old_locus_tag="pgaptmp_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGV7848817.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(137636..138202)
                     /locus_tag="ID725_RS00640"
                     /old_locus_tag="pgaptmp_000128"
     CDS             complement(137636..138202)
                     /locus_tag="ID725_RS00640"
                     /old_locus_tag="pgaptmp_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGV7848818.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            138680..139468
                     /gene="fabI"
                     /locus_tag="ID725_RS00645"
                     /old_locus_tag="pgaptmp_000129"
     CDS             138680..139468
                     /gene="fabI"
                     /locus_tag="ID725_RS00645"
                     /old_locus_tag="pgaptmp_000129"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGV7848819.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            139612..140739
                     /gene="yciW"
                     /locus_tag="ID725_RS00650"
                     /old_locus_tag="pgaptmp_000130"
     CDS             139612..140739
                     /gene="yciW"
                     /locus_tag="ID725_RS00650"
                     /old_locus_tag="pgaptmp_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGV7848820.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            140807..142741
                     /gene="rnb"
                     /locus_tag="ID725_RS00655"
                     /old_locus_tag="pgaptmp_000131"
     CDS             140807..142741
                     /gene="rnb"
                     /locus_tag="ID725_RS00655"
                     /old_locus_tag="pgaptmp_000131"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGV7848821.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(142860..142922)
                     /locus_tag="ID725_RS00660"
                     /old_locus_tag="pgaptmp_000132"
                     /pseudo
     CDS             complement(142860..142922)
                     /locus_tag="ID725_RS00660"
                     /old_locus_tag="pgaptmp_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            142976..144956
                     /gene="pdeR"
                     /locus_tag="ID725_RS00665"
                     /old_locus_tag="pgaptmp_000133"
                     /pseudo
     CDS             142976..144956
                     /gene="pdeR"
                     /locus_tag="ID725_RS00665"
                     /old_locus_tag="pgaptmp_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(144938..145066)
                     /gene="ymiD"
                     /locus_tag="ID725_RS00670"
                     /old_locus_tag="pgaptmp_000134"
     CDS             complement(144938..145066)
                     /gene="ymiD"
                     /locus_tag="ID725_RS00670"
                     /old_locus_tag="pgaptmp_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGV7848822.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            145104..145277
                     /gene="yciZ"
                     /locus_tag="ID725_RS00675"
                     /old_locus_tag="pgaptmp_000135"
     CDS             145104..145277
                     /gene="yciZ"
                     /locus_tag="ID725_RS00675"
                     /old_locus_tag="pgaptmp_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGV7848823.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            145367..146116
                     /gene="yciT"
                     /locus_tag="ID725_RS00680"
                     /old_locus_tag="pgaptmp_000136"
     CDS             145367..146116
                     /gene="yciT"
                     /locus_tag="ID725_RS00680"
                     /old_locus_tag="pgaptmp_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGV7848824.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            146385..146603
                     /gene="osmB"
                     /locus_tag="ID725_RS00685"
                     /old_locus_tag="pgaptmp_000137"
     CDS             146385..146603
                     /gene="osmB"
                     /locus_tag="ID725_RS00685"
                     /old_locus_tag="pgaptmp_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGV7848825.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(146729..147055)
                     /gene="yciH"
                     /locus_tag="ID725_RS00690"
                     /old_locus_tag="pgaptmp_000138"
     CDS             complement(146729..147055)
                     /gene="yciH"
                     /locus_tag="ID725_RS00690"
                     /old_locus_tag="pgaptmp_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGV7848826.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(147055..147792)
                     /gene="pyrF"
                     /locus_tag="ID725_RS00695"
                     /old_locus_tag="pgaptmp_000139"
     CDS             complement(147055..147792)
                     /gene="pyrF"
                     /locus_tag="ID725_RS00695"
                     /old_locus_tag="pgaptmp_000139"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGV7848827.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(147985..149154)
                     /gene="lapB"
                     /locus_tag="ID725_RS00700"
                     /old_locus_tag="pgaptmp_000140"
     CDS             complement(147985..149154)
                     /gene="lapB"
                     /locus_tag="ID725_RS00700"
                     /old_locus_tag="pgaptmp_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGV7848828.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(149161..149469)
                     /gene="lapA"
                     /locus_tag="ID725_RS00705"
                     /old_locus_tag="pgaptmp_000141"
     CDS             complement(149161..149469)
                     /gene="lapA"
                     /locus_tag="ID725_RS00705"
                     /old_locus_tag="pgaptmp_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGV7848829.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(149618..150382)
                     /gene="pgpB"
                     /locus_tag="ID725_RS00710"
                     /old_locus_tag="pgaptmp_000142"
     CDS             complement(149618..150382)
                     /gene="pgpB"
                     /locus_tag="ID725_RS00710"
                     /old_locus_tag="pgaptmp_000142"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGV7848830.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            150552..151142
                     /gene="ribA"
                     /locus_tag="ID725_RS00715"
                     /old_locus_tag="pgaptmp_000143"
     CDS             150552..151142
                     /gene="ribA"
                     /locus_tag="ID725_RS00715"
                     /old_locus_tag="pgaptmp_000143"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGV7848831.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(151206..153881)
                     /gene="acnA"
                     /locus_tag="ID725_RS00720"
                     /old_locus_tag="pgaptmp_000144"
     CDS             complement(151206..153881)
                     /gene="acnA"
                     /locus_tag="ID725_RS00720"
                     /old_locus_tag="pgaptmp_000144"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGV7848832.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDCEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(154045..154140)
                     /gene="ymiC"
                     /locus_tag="ID725_RS00725"
                     /old_locus_tag="pgaptmp_000145"
     CDS             complement(154045..154140)
                     /gene="ymiC"
                     /locus_tag="ID725_RS00725"
                     /old_locus_tag="pgaptmp_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGV7848833.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(154254..154421)
                     /gene="yciX"
                     /locus_tag="ID725_RS00730"
                     /old_locus_tag="pgaptmp_000146"
     CDS             complement(154254..154421)
                     /gene="yciX"
                     /locus_tag="ID725_RS00730"
                     /old_locus_tag="pgaptmp_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGV7848834.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(154424..154564)
                     /gene="ymiA"
                     /locus_tag="ID725_RS00735"
                     /old_locus_tag="pgaptmp_000147"
     CDS             complement(154424..154564)
                     /gene="ymiA"
                     /locus_tag="ID725_RS00735"
                     /old_locus_tag="pgaptmp_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGV7848835.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(154883..155857)
                     /gene="cysB"
                     /locus_tag="ID725_RS00740"
                     /old_locus_tag="pgaptmp_000148"
     CDS             complement(154883..155857)
                     /gene="cysB"
                     /locus_tag="ID725_RS00740"
                     /old_locus_tag="pgaptmp_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGV7848836.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(156067..158664)
                     /gene="topA"
                     /locus_tag="ID725_RS00745"
                     /old_locus_tag="pgaptmp_000149"
     CDS             complement(156067..158664)
                     /gene="topA"
                     /locus_tag="ID725_RS00745"
                     /old_locus_tag="pgaptmp_000149"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGV7848837.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            159044..159295
                     /gene="yciN"
                     /locus_tag="ID725_RS00750"
                     /old_locus_tag="pgaptmp_000150"
     CDS             159044..159295
                     /gene="yciN"
                     /locus_tag="ID725_RS00750"
                     /old_locus_tag="pgaptmp_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGV7848838.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(159331..160380)
                     /gene="sohB"
                     /locus_tag="ID725_RS00755"
                     /old_locus_tag="pgaptmp_000151"
     CDS             complement(159331..160380)
                     /gene="sohB"
                     /locus_tag="ID725_RS00755"
                     /old_locus_tag="pgaptmp_000151"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGV7848839.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            160600..161358
                     /gene="yciK"
                     /locus_tag="ID725_RS00760"
                     /old_locus_tag="pgaptmp_000152"
     CDS             160600..161358
                     /gene="yciK"
                     /locus_tag="ID725_RS00760"
                     /old_locus_tag="pgaptmp_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGV7848840.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            161355..161945
                     /gene="cobO"
                     /locus_tag="ID725_RS00765"
                     /old_locus_tag="pgaptmp_000153"
     CDS             161355..161945
                     /gene="cobO"
                     /locus_tag="ID725_RS00765"
                     /old_locus_tag="pgaptmp_000153"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGV7848841.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(161985..162857)
                     /gene="rluB"
                     /locus_tag="ID725_RS00770"
                     /old_locus_tag="pgaptmp_000154"
     CDS             complement(161985..162857)
                     /gene="rluB"
                     /locus_tag="ID725_RS00770"
                     /old_locus_tag="pgaptmp_000154"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGV7848842.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(163070..164653)
                     /locus_tag="ID725_RS00775"
                     /old_locus_tag="pgaptmp_000155"
     CDS             complement(163070..164653)
                     /locus_tag="ID725_RS00775"
                     /old_locus_tag="pgaptmp_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGV7848843.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            complement(164681..165301)
                     /gene="yciO"
                     /locus_tag="ID725_RS00780"
                     /old_locus_tag="pgaptmp_000156"
     CDS             complement(164681..165301)
                     /gene="yciO"
                     /locus_tag="ID725_RS00780"
                     /old_locus_tag="pgaptmp_000156"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGV7848844.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(165298..166179)
                     /gene="rnm"
                     /locus_tag="ID725_RS00785"
                     /old_locus_tag="pgaptmp_000157"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(165298..166179)
                     /gene="rnm"
                     /locus_tag="ID725_RS00785"
                     /old_locus_tag="pgaptmp_000157"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGV7848845.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            166317..166361
                     /gene="trpL"
                     /locus_tag="ID725_RS00790"
                     /old_locus_tag="pgaptmp_000158"
     CDS             166317..166361
                     /gene="trpL"
                     /locus_tag="ID725_RS00790"
                     /old_locus_tag="pgaptmp_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGV7848846.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    166338..166431
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            166453..168015
                     /gene="trpE"
                     /locus_tag="ID725_RS00795"
                     /old_locus_tag="pgaptmp_000159"
     CDS             166453..168015
                     /gene="trpE"
                     /locus_tag="ID725_RS00795"
                     /old_locus_tag="pgaptmp_000159"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGV7848847.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVGPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            168015..169610
                     /gene="trpD"
                     /locus_tag="ID725_RS00800"
                     /old_locus_tag="pgaptmp_000160"
     CDS             168015..169610
                     /gene="trpD"
                     /locus_tag="ID725_RS00800"
                     /old_locus_tag="pgaptmp_000160"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGV7848848.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            169611..170972
                     /gene="trpCF"
                     /locus_tag="ID725_RS00805"
                     /old_locus_tag="pgaptmp_000161"
     CDS             169611..170972
                     /gene="trpCF"
                     /locus_tag="ID725_RS00805"
                     /old_locus_tag="pgaptmp_000161"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGV7848849.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            170984..172177
                     /gene="trpB"
                     /locus_tag="ID725_RS00810"
                     /old_locus_tag="pgaptmp_000162"
     CDS             170984..172177
                     /gene="trpB"
                     /locus_tag="ID725_RS00810"
                     /old_locus_tag="pgaptmp_000162"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGV7848850.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            172177..172983
                     /gene="trpA"
                     /locus_tag="ID725_RS00815"
                     /old_locus_tag="pgaptmp_000163"
     CDS             172177..172983
                     /gene="trpA"
                     /locus_tag="ID725_RS00815"
                     /old_locus_tag="pgaptmp_000163"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGV7848851.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            173364..173543
                     /gene="yciG"
                     /locus_tag="ID725_RS00820"
                     /old_locus_tag="pgaptmp_000164"
     CDS             173364..173543
                     /gene="yciG"
                     /locus_tag="ID725_RS00820"
                     /old_locus_tag="pgaptmp_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGV7848852.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            173629..174129
                     /gene="yciF"
                     /locus_tag="ID725_RS00825"
                     /old_locus_tag="pgaptmp_000165"
     CDS             173629..174129
                     /gene="yciF"
                     /locus_tag="ID725_RS00825"
                     /old_locus_tag="pgaptmp_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGV7848853.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            174175..174681
                     /gene="yciE"
                     /locus_tag="ID725_RS00830"
                     /old_locus_tag="pgaptmp_000166"
     CDS             174175..174681
                     /gene="yciE"
                     /locus_tag="ID725_RS00830"
                     /old_locus_tag="pgaptmp_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGV7848854.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(174741..174866)
                     /locus_tag="ID725_RS00835"
                     /old_locus_tag="pgaptmp_000167"
                     /pseudo
     CDS             complement(174741..174866)
                     /locus_tag="ID725_RS00835"
                     /old_locus_tag="pgaptmp_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            175090..175191
                     /locus_tag="ID725_RS00840"
                     /old_locus_tag="pgaptmp_000168"
                     /pseudo
     CDS             175090..175191
                     /locus_tag="ID725_RS00840"
                     /old_locus_tag="pgaptmp_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(175601..175836)
                     /locus_tag="ID725_RS00845"
                     /old_locus_tag="pgaptmp_000169"
                     /pseudo
     CDS             complement(175601..175836)
                     /locus_tag="ID725_RS00845"
                     /old_locus_tag="pgaptmp_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(175995..>176204)
                     /locus_tag="ID725_RS00850"
                     /old_locus_tag="pgaptmp_000170"
     CDS             complement(175995..>176204)
                     /locus_tag="ID725_RS00850"
                     /old_locus_tag="pgaptmp_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGV7848855.1"
                     /translation="QRGVFLRNAEGSRVCSLYDEMALSRIINDGMHHPLSREPITLSM
                     LVAREQCEFDCSIGHFTVRSDCYSV"
BASE COUNT        43981 a        45688 c        42292 g        44243 t
ORIGIN      
        1 aaacattttg agattataga atacctgtga taacaagtga aacatctgca caatgtgaat
       61 agttagtttc ttttcagagg aaatgaaata gacaagctga atgaaaggga gtgtgatata
      121 aattaaaaaa cataacatga atctaattaa ttacgcacat cattgtggat aaagttactg
      181 aataataaat aacataatct tctggtatcc cactcaaata atttcattcc tttttagtat
      241 ttcttgtaac gttggtaact gacatactgc atcagcaatc gcactgatat tcgtggcatt
      301 atcctgaaac cattcatgcc caggccccca tgtgcgcata gtgcaaagat aacagtccac
      361 cagcgttagt tgctcaccaa acgcataagg ttcagcggtc aattgtgaat tcagccagac
      421 ataaagcgat ttacgatatt caataacatt cttctttaac tgttcaggtg cgtcagatgc
      481 ccaacgctca gggtaatcgg cgaaagtgaa cgttggatag acattagcaa ccagccagac
      541 caataaccgt tgaaactgct gacgttcggc acgcccaacc ggtggcgcaa ggtccgggcg
      601 acgatcgaga accatcaatg caatcgccgc tgtctccgtc ataatctcgt cattttccag
      661 tgccagtgtc ggaacctgac acaacgggtt cagggttttc aataactcgc gtgaagttcc
      721 ctcgtggtca aaaccactga catcaacgaa ctgataagga atatcagcca gtgttagcat
      781 cagctcactg attgtagagc cccatccggg tacgccatac accttaatca tatgcccctc
      841 acaatctttt aaagattaaa tgtaggacag tacaaaggtt aaccagcaaa aggatttgtc
      901 attttttcgt cagttgtatt aatccccttc aggattgtaa ggggatgttt tgcatcgttt
      961 atcgataacg ggtaggatgc tgcgccgata gttttatacc cataaagaat aatggtgata
     1021 actatcatcg ccaggatgaa taaacattgt tcatggcaac ttatatgact ttttcattaa
     1081 agcaatcagg gagagcaatg agtaaacacg acaccgacac ttcagatcaa cacgccgcga
     1141 aacgccgctg gcttaatgcc cacgaagagg ggtatcacaa agcgatgggc aatcgccagg
     1201 tacagatgat cgccattggc ggcgcgattg gcaccggctt gtttttaggt gcaggagccc
     1261 gactgcaaat ggcgggtcca gcactggcac tggtttattt aatttgtggc ttgttttcgt
     1321 tttttattct gcgtgcattg ggtgagctgg tgctacaccg cccttccagt ggcagttttg
     1381 tctcttatgc ccgtgagttt ttgggtgaga aagccgctta tgttgctggc tggatgtact
     1441 tcatcaactg ggcgatgacg gggattgttg atattaccgc cgtcgctctg tatatgcatt
     1501 actggggtgc gtttggcggc gtgccgcagt gggtctttgc gctcgctgca cttaccatcg
     1561 ttggcaccat gaatatgatc ggtgtgaaat ggtttgcgga gatggagttc tggtttgcgc
     1621 ttattaaagt gctcgccatt gtgacctttt tggtcgtggg tacggtgttc ctcggtagtg
     1681 gtcagccgct ggatggcaac accactggct ttcatttaat caccgataat ggcggcttct
     1741 tcccccacgg tttgctgcct gcgctggtgt tgattcaggg cgtagtgttt gcttttgcct
     1801 ccattgaaat ggtgggtaca gctgccggag aatgtaaaga tccgcagacc atggtgccta
     1861 aagcgattaa cagtgtgatt tggcgtattg gcctgtttta cgtcggctcc gtggtgttgc
     1921 tggttatgtt attgccgtgg agcgcgtatc aggcggggca aagtccgttc gtgacgtttt
     1981 tctctaaact gggtgtgcca tatatcggca gcattatgaa cattgtggtg ctgaccgctg
     2041 ccctctccag cctgaattca ggtctgtact gcaccggacg tattctgcgc tcaatggcga
     2101 tgggcggttc cgcaccgagt tttatggcga aaatgagtcg tcagcatgtg ccgtatgccg
     2161 ggattctggc gacactggtt gtgtatgtcg tcggcgtatt cctcaactat ctggtgccgt
     2221 cgcgcgtatt tgagattgtg ttgaacttcg cgtcgctggg aatcatcgct tcatgggcgt
     2281 ttatcatcgt gtgccagatg cgcctgcgta aagcgattaa agaaggcaaa gcagcggatg
     2341 tcagttttaa actgcctggc gcgcccttca cttcctggct gacattactg tttttactga
     2401 gtgtccttgt gctgatggcg ttcgattacc cgaacgggac ttatactatc gcggcgctgc
     2461 cgattatcgg tattctgctg gttataggct ggtttggtgt gcgcaaacgc gttgctgaaa
     2521 ttcacagcac tgcgccagtc gtcgaggaag atgaagaaaa acaggaaatt gtgtttaagc
     2581 ctgaaacggc gagctaattg caaaattgcc cggctacgtg ccgggcaaat ttagattact
     2641 gacgaaggtg tgccgcatgc ggcgtgatca aagtcaggca agtctctccg ttatcaccct
     2701 cgccatatct attacagcgc aatacgaatc acatcgtctg gctgggtagc ttcctgctgt
     2761 ttagtggatt tttgtttcac actgacatac agcgttttgc catcggcaga cagcgccagg
     2821 ctgttcggat gagtcggcgt atcgaacgtt ttcaccactt tatagctttt cgcgtcaatc
     2881 acactgactt tacctgcctg acgatgcgtt acgtaggctt cattacgcgc tgggttaaac
     2941 agcacagcca gtgattccgg tgccgcaacc ttcgccagaa tattgccatt acgggtatcg
     3001 accactaaca cttctgccgc tttagaatcg gtgataaatg cacgctgcct ggcggtatca
     3061 aggctgatgt tgataaagaa gtgctctttg ccgtcatcca gcagcttttt acggctgagg
     3121 attttattgt cggcggtgtc gatggtaatc aattcgccgt cagcgttagt ggtgtaaaga
     3181 cgtttgcctt tgctatccag tgccagaccg gtactcattt taccggtgtt ctggatggcg
     3241 gttttcagtt tgatattctc gccatcaacg acccaaatca cgctatcttt accaatacca
     3301 ctgatgtaaa cggtgttcgt ggcatcatca gctaccagct cacgcggttg cagcgggcgc
     3361 acctcttccg tgcgcttacg atcatccagc accagacggc ctttcacctc gccagttttg
     3421 gcatctatcg ccgtgaccgc gctgtttacg gtgttaccaa accacaacgt ctgagtcgtg
     3481 ttattgatgg tggcaccaaa cggcttgaga tcgttatgga tcgcctgcgt cacttccaga
     3541 gtgaccggat caagacgata aaccacgccg cctttatcca gtttgcggct ttgcgaagtg
     3601 gcgagccaca gcgcgttttc ttgctggcta taagccattt cgtaggcacc tttacctacc
     3661 gctttacgca gcatttcttc tgcggcctgc gtactgaatg atgaagcaac aagcaatgaa
     3721 cctaacagta atgaaccacg caggcgcaat gaaaacagat gacgtaaatg catgacgact
     3781 ccctttgatg aaccgatatt tttgctgctt cacaatgctt cgctaagcgt aatcatcgtt
     3841 acgctcttgt tataaatggg aatggtaatc attatttact caaatgtgga gcatttcttg
     3901 ccgttacgac tacaattctc tcaattaatt aacgtatcgt gccgtttcgt tttccataaa
     3961 tttacaattt cattaccatg acggtgtttt cccattctta acctggcttt tttcatgaag
     4021 attgttttcg ttcgacagac cgttttgccc gccctgcttg tcctttcccc cgttgttttt
     4081 gccgctgatg aacagaccat gattgtcagt gccgcgccgc aggtggtttc agaactggat
     4141 acccctgcag cagtaagcgt ggtggatggc gaggagatgc gcctggcaac accgcgcatt
     4201 aacttgtccg aatcactgac cggcgtgcct ggtttgcagg tacaaaaccg gcagaactat
     4261 gcgcaagatt tacagctgtc gattcgtgga tttggctccc gctccactta cggaattcgc
     4321 ggtattcgcc tgtatgtgga cggtattccc gctaccatgc ccgacgggca agggcaaaca
     4381 tccaacatcg atttaagcag tgtgcaaaat gtggaagtgc tgcgtggccc cttctctgcc
     4441 ctgtatggca acgcgtctgg cggtgtaatg aatgtcacca cccagaccgg gcaacagcca
     4501 ccaaccattg aagccagtag ttactacggc agttttggca gctggcgcta tgggctgaaa
     4561 gcaacgggcg caacgggaga cggcacacag cctggcgatg ttgattacac cgtctcaacc
     4621 acgcgtttta cgacccacgg ctatcgtgac catagtggcg cacagaaaaa tttagctaat
     4681 gccaaactgg gcgtacgcat tgatgacgcc agcaaattaa gcctgatttt caatagcgtg
     4741 gatatcaaag cagatgaccc aggtgggcta accaaagcag aatggaaagc gaatccgcaa
     4801 caagcgcctc gtgcagaaca gtacgacacg cgaaaaacca tcaagcaaac tcaggctggg
     4861 ttgcgctatg aacgtagcct gagttcgcgg gatgatatga gtgtgatgat gtatgccgga
     4921 gagcgagaaa cgacccagta ccagtcaata cccatggcac cacaacttaa cccgtcacat
     4981 gcgggcagcg tgattaccct gcaacgccat taccagggaa tagacagccg ctggacacac
     5041 cgtggtgaac tgggcgttcc ggtcacgttc actaccggcc tgaactacga aaacatgagt
     5101 gaaaaccgca agggctacaa taacttccgc ctgaatagcg gcatgccgga gtacgggcaa
     5161 aaaggtgagt tgcgtcgcga cgaacgcaat ctgatgtgga acatcgatcc ctatttacag
     5221 acgcagtggc agctgagcga aaaactgtcg ctggatgctg gcgtgcgcta cagctccgtg
     5281 tggtttgatt ccaacgacca ttacgttact ccgggtaacg gcgatgacag cggtgatgcc
     5341 agttatcaca aatggctacc agccggatcg ttaaaatatg caatgaccga tgcctggaat
     5401 atctatctgg cagccgggcg tggttttgaa acgccgacga ttaatgagct gtcttatcgc
     5461 gctgatgggc aaagcggtat gaactttggt ttaaaaccat ccaccaacga tacaattgag
     5521 atcggcagta aaacgcgtat tggtgatggg ctgcttagtc tcgcattgtt ccagaccgac
     5581 accgatgatg aaattgttgt cgatagcagt agcggtgggc gtacgactta caaaaatgct
     5641 ggaaagaccc gtcgtcaagg cgctgaactg gcatgggatc aacgtttcgc gggagatttt
     5701 cgcgtaaacg cgtcctggac ctggcttgat gcgacctatc gcagcaatgt ttgcaacgaa
     5761 caggattgta acggtaatcg gatgccaggg atcgcccgta atatgggctt tgcgtcgata
     5821 ggttatgtac cggaagatgg ttggtatgca ggcatggaag cgcgttatat gggcgatatt
     5881 atggcagatg atgaaaatac ggccaaagcg ccgtcttata ctctcgtcgg cttattcacc
     5941 gggtataaat acaattacca caatttaact gtggatttgt ttggtcgtgt cgataattta
     6001 ttcgataaag aatacgttgg ttctgtcatt gtcaatgagt caaacgggcg atattacgaa
     6061 cctgcgcccg ggcgaaatta tggtgtcggc gtgaatatcg catggcgatt tgagtaatta
     6121 ccttgatgcc cggtatttgc cgggcattta ctttaacgat tgtattgctg gtataaaata
     6181 gcaatatttt gttgcataat ttcagcaaga caatgtctgc atccctcttt gtcttttgct
     6241 tttaacgcag cgagtaacag atgatagttt tctatatgct cccgtaattc cgctggatta
     6301 atcgcttcat agagataatg taaaccaggt cccatcctga cccacagttg ctcaatcatc
     6361 tcacacagga tgggcatatt tgagcgatga taaatcgcta agcgaaatag cctgtttaca
     6421 ttaatgattt gttccatgtc gcccttttct tgcgcctgct gtaatttctc gagcaattcc
     6481 tggagttccg ccaggtcttg cggggtgagg ttttcaacag ccagagcaac tgccattaat
     6541 tccagttcgt agcggatccg attgatttcg tctaattgac gtttccccac ttccggaact
     6601 gtaaatgctt gcgctggtgc gacagaaagc gcattcaccg aaaccagacg taataatgct
     6661 tcacgcacag gtgtaatact catacctaat tgctccgcca gatttttagt aatcaggcgt
     6721 gcgccaggtt ttagtgcgcc aatactaagc tgatgtttca ggtcgttctc aacctgcaga
     6781 gtcaaactga catgtttcat tttttccgtt ccaggcattt taattccttc gcgtgttttc
     6841 tgtccaaccc tgcctgcatg gcagaaggaa ttcacctggt gtattaaagt gaataatcat
     6901 aaaccactca cctttgttgt tattagcagc agaacaatga tgtggttagt taattaaagc
     6961 tgaaaataag ctttttgcct ggatttttat ggcggcttac gccgccgttg aaactttaat
     7021 catcacccgc cacgcggatc accactttgc cgaagttttt acccttcagc aggccgataa
     7081 acgtctgtgg cgcattctct aaaccgtcag taatttcttc gcggtagtgg attttatcct
     7141 ctttcaccca ttgccccatc tccctctgaa actcatggat gcggtgacca taatcctgag
     7201 cgataataaa accttgcaag cgaatacgtt ttttcagcac tgtagccatc aacagaggta
     7261 aacgatccgg accgggtggt agctctgtag cgttatagct gctcactaat ccgcagacgg
     7321 gaatgcgcgc agatgtatta agtaacggta gcaccgcatc gaatacctta ccgcccacgt
     7381 tttcataata gatatcaata ccttttgggc acgctttcgc cagttgttcg gcaaaatcat
     7441 ccgcgtggtg atcaagacaa acatcgaagc ctaacacctc ggtagcatgg cggcattttt
     7501 ccgcgccacc ggctaccccc accactctgc aacctttaag tttgccgatt tgccccaccg
     7561 tcgcccccac tggtcctgtc gccgcagcta ccaccaacgt ttcgccctct ttaggctgac
     7621 cgatatccag taggcccata taagcggtaa agcctggcat ccctagcaca cccagcgacc
     7681 acgatggatt ttgcggatga tcgccaagtt tcaccagatc atcaccactg gatatgtcat
     7741 agtcttgcca tccactgtag cccagcaccc agtcgccaga ctgataatca ggatgattcg
     7801 actccacgac acggctcacc gtaccgccga ccatcacgcc gccaatatca accggcggtg
     7861 aataagatgg ctcatcgctc atacgtccac gcatatacgg gtccagggac aaataaactg
     7921 tgcgcagtaa cacctgtcct tcacccggtg tggcgacatc atcttcttca agacggaaat
     7981 tctccggaac cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga ttacgctgct
     8041 tttgttgccc cataatgctc tcctgtgctt ttggcatgaa tccatcaata gtagcaggct
     8101 cgcaggattg gttttgtttg cagccgtcac cccccctctg caacacgcaa taatttcatc
     8161 gaggaatccg ttgcttcggg ctgttttcga tagtattgat aacatcatca tcattatttt
     8221 attgaggcca gacatgtcca tccgttttgc ccgcaaagcc gactgtgctg ccattgcgga
     8281 aatttataac cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa cggtggatgc
     8341 agataaccgc attgcctggt ttgaagcgcg gaccatagca ggttatccgg tgctggtaag
     8401 tgaagaagat ggcgtagtga cgggatatgc ctcatttggc gactggcgta gtttcgatgg
     8461 ttttcgccat accgtggaac attcggttta tgtccatccc gatcatcagg gcaaaggtct
     8521 ggggcgtaaa ttgttaagcc gattgattga tgaagcgcga gattgcggga agcatgtcat
     8581 ggtcgccggg atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccagt cgctgggatt
     8641 tgtcgtcacc gcacaaatgc cgcaggtggg cactaaattt gggcgctggc tggatctgac
     8701 atttatgcag ttgcaactcg acgaacgcac tgaaccggac gcgattggat gaatcagtcg
     8761 ctcacccttg cctttttgat tgccgccgga attggtctgg tagtccagaa ctcgctgatg
     8821 gtgcgcatca cccagacctc ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg
     8881 gggattgtgc tctttgtttc tattttatgg tttaaacaag gtatggcggg gtttggcgaa
     8941 ctggtgtcca gcgtgcgctg gtggacactt atacctggct tgctgggatc gttttttgtc
     9001 ttcgccagta tcagcggata tcagaatgtg ggagcggcga caactattgc agtactggtc
     9061 gccagccagc tgattggcgg tctggttctg gatatcttcc gcagtcatgg cgtaccacta
     9121 cgggcgttgt tcggcccaat ttgtggcgca attttacttg tggttggcgc ctggttggta
     9181 gccagacgct cattttaatc acaggatggt gccgcctttg gttaactgtt catggcgcgc
     9241 ttccatatct tctttatgct tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg
     9301 ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg ctcagggcga ttaataattc
     9361 gttcatatgg gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc
     9421 cagatgcgac atactttctc cttagccact tgtgaaattt atcgattcat ccaaatgata
     9481 taagtctgcg tggtgccgtc aagccagtga gtaagattta aaacagtgaa aacaacagcg
     9541 ccaccgggaa actcatcggc catgttgccc ccaccagaaa ggcgcttaag aaacggatgc
     9601 gtttcttatc gtgagaaaga aaccaagtga taagaaatgc gattgctgcc ataaccgcat
     9661 aaaaaacaag catgtgttga tagagagaca tgtacgggcg tccagccaga tgaataaaac
     9721 gcgcgcaaat atgcgtcatt ttttgacgtg catcaagttt tttaaccggt ttgagaaaaa
     9781 aacatcaaac agcaacttgc ccttatccat cttcagaatg agtattattg cggattttct
     9841 taacccgtac tatacacata ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt
     9901 agaactgtag tactgataaa tgtctttgct gctgttggtt tgtttactct tatctctatg
     9961 agatttggct ggtttatttg atgtagtgat tttgcacgca gcacgtactg tcagagaaat
    10021 cagtgctctg acagtacgca agatcatctc atactcttac gatctgcaat attatcctta
    10081 atgtttaacc atgacgtggc ggacgacggt gtaatcctcc agcccataaa gtgacatatc
    10141 cttgccgtaa ccagaaagtt tctgcccacc gtgcggcatt tcacttacca gcatgaaatg
    10201 ggtattgacc caggtacaac catattgcag ccgtgcactg acgcgatgcg ccctgcccac
    10261 atctttcgtc cataccgaag atgcaagtcc gtactggctg tcattcgccc agtttaccac
    10321 ctgttcttcg ttgtcgaaga gcgtaacact cactactgga ccaaatacct ctttttgcac
    10381 gatggcatcg tcctgtaatg cgccagccag cagcgtcggc gcatagtaat agccattacc
    10441 cttgcgcttt tcaccgccag tgatcacttt gatgtgccct gtcgctttcg cctcttctac
    10501 tgccttgctg acgcgttcga gatgcgccag cgagcttaaa ggtccaagct ccgtagactc
    10561 gtcatccggc gcaccagatt ttaacgttgc cactgcagca ccgagttttt ccaccagcgt
    10621 atcgtaaatg cctttttgcg cgtagatccg acaagccaca gtacaatcct gtccggcatt
    10681 gtaatagcca aatgtacgta caccttcgac cactgcttca atatccgcat catcaaaaac
    10741 aatcactggc gctttgccac caagttccat atgagtacgc ttaatggagg gcgcggtatg
    10801 gctgatgatg tgctcgccgg tggcgataga gcccgtcagc gacaccatcc gcactttggg
    10861 atgaccggtc agcggatcgc ccaccgtttt gcctctgcca aacagtacgt taatcacgcc
    10921 tgccgggaag atatctttcg ccagctctgc caacttcaac gcggtcagcg gggtaatttc
    10981 tgatggttta agcactacgc agttccctgc cgccagcgcc ggagccagtt tccacgctgc
    11041 catcatcagc ggataattcc acggtgcgat agaagccacg acccccaacg gatcgcgacg
    11101 gatcatcgaa gtatgacctt caagatattc acctgccgcc agaccattca gacagcgcgc
    11161 cgcacccgcg aaaaagcgaa aaacatcgac aatcgccggg atttcatcat tgaacgcact
    11221 atgcagcggt ttgccacaat tacgggactc cagttcggca aaaacctgac cattttcttc
    11281 gataacatca gccagtttca gcagacattc cgcacgcact ttcggcgtgg tttgccccca
    11341 ttcggcaaat gctgcatctg ccgcgcgcac agcagcatcg acctgctctg cggatgcctc
    11401 ggcaatctcc agtaaaacgt cccccgttgc cggattatag acaggctgtt tttccccttc
    11461 gccgctaacc agttctccgt taatcagtaa cttatgttgc atagcatatt cctatatcag
    11521 tggttattga ccattgtcgc cttcgcgggt tagccaccag gctcccagga tcggcaaggt
    11581 tgttaccaac ataaccagca gtgccaccac gttagttacc ggtacatcac gcggtcgccc
    11641 aagctgattg agcaaccaca acggtaacgt tcgttcatga cctgccgtaa aggtcgtaac
    11701 gatgatttca tcgaacgaca aggcaaacgc cagcattcct cctgccagta acgccgaact
    11761 gagattcggc aacactacgt agcggaaggt ttgccagcca ttggccccaa gatccattga
    11821 cgcctcaacc agactccatg aggtgcggcg aaaacgggcg atgacattgt taaacaccac
    11881 aactacacaa aaagtcgcat gaccgaccac gatggtgaaa aatcccgact ccagattgat
    11941 ggttttaaag gcggttaata acgccagacc agtgacaatg cccggcagcg caatgggcag
    12001 cagcagtaac agcgaaatgg cgtttttgcc gaaaaagtct cgtcgccaca gcgcggcagc
    12061 tgccagcgtc cctaacacca gcgccattaa tgtcgccagc gccgccactt taagtgacag
    12121 tgtcacggca tcaagaatat cactacgctg tgctgccacg ctaaaccagc gcagcgtcag
    12181 gccctgcggt ggaaaactaa acgccgcatc ttcagtgtta aacgcatagg cggcgattat
    12241 caggatggga aaatgtagga aaacaacgcc gccccaggcc gccagtttga ggaaaaacgg
    12301 tgcgcgttca gagtgcatca aacgctccca gacgtttcac gaacgccagg tacagtgcga
    12361 taagaacaat cggcaccagg gtgaatgccg ccgccatcgg catattgcca atcgccccct
    12421 gctgggaata aaccatattg ccgataaaat atcctggcgg gccaaccagc tgcgggacga
    12481 taaaatcgcc cagtgtgagt gagaaggtaa agatagagcc agcggcaata cccgggatcg
    12541 ccagcggcaa caccacatag cgaaaggttt gtcgtggacg tgcgccgaga tcagctgacg
    12601 cctgcaacaa tgacggcggc aaacgctcaa gcgccgcctg cacgggcagg atcatgaacg
    12661 gcagccagat atagagaaac accagaaagc gccccagccc ggaagttgac agcgtattgc
    12721 cgcccaccgc aggtaatgta aggaacgctg tcagcagtgg ttccagccca agatgttgta
    12781 aaaaccactg agccacgcca tcttttgcca gcaataacgt ccaggcatag gctttaacaa
    12841 tgtagctcgc ccacatcggc aacattaccg caatataaaa aaacgctttc attttcccgc
    12901 tggtatagcg cgccatatac cacgccattg gaaaagccag aatggcgctg gcgatagtga
    12961 ctgccacagc catagtcagc gtgcggagaa tgatgtcgta attcgccgga ttaaacagcg
    13021 cacggatgtt cgccagcgtc agttccggcg ttaccgacat ggtgaaatcg tcaaaggtat
    13081 aaaagccctg ccataacagc gttagcagcg agccgaaata gacaatgcca aaccacatta
    13141 gcgggccaag cagcagcaaa aacagcccca gccccggatt ccgccagaag aaaccggaca
    13201 ccttacccag acctggacgt gaaggtgatt gcaatacgtt catcgccatt cacctctcct
    13261 caaccagcgg caccatcaca tcacgcgacc aggaaaccat cacctgttgt ccgggcgtga
    13321 gcgtggcagg cagttcttcg cctgtcatat tggcctgact caccagcagt ttttcaccgc
    13381 cgttcaattt cagttcaaaa cgagttgccg cgccctgata ttgcaccgcc tggatcgtgc
    13441 cattggcctg cagttcacca ggggtgttga ggcggatatg ttccggccgc agggcgaagc
    13501 ttcccgtcat gccacaaagt ttctctgcca tcagtccatc aaaaacattc gatgtaccaa
    13561 cgaacccggc aacaaacggc gtgcgcgggc gcatatagag atcgcgcggg gaatcgacct
    13621 gctcaatgcg tccattattg aaaaccgcca cacgatcgga catcgataac gcttcgccct
    13681 gatcgtgggt aacgcagata aaagtgatac cgagagactg ttgcagtttt ttcagctcaa
    13741 gctgcatctg ctcacgcaat ttgagatcca gtgcgccgag cggttcatcc aacagcaata
    13801 cgcgcggttc attcaccaat gctctggcga tagcaacccg ctggcgctga ccaccagaaa
    13861 gttgtgacgg tttacgttgc tgtacaaacc ccaacgccac tttctccagc gcctcttgcg
    13921 ccattgcgtg ccgctgcttt ttattcacgc ctttgaccat cagcccatag gcgacattgt
    13981 caagaatcga catatgcgga aatagcgcgt agtcctgaaa gacagtattc acgtcccgct
    14041 cccacggtgg cagattgctg gcgggtttac caaagataga gatagccccg ccggaaagct
    14101 gttcgaagcc agcaatcagg cgcaggcagg tggttttgcc ggagccggac ggccccagca
    14161 tagagaagaa ctcaccatct tttatcgcaa tactgacgcc atctactgcg cgcacgtcac
    14221 cgtacaaccg cgagacgttg tcaaactcca ctgcgtacgt cataaagcac cccagcgaag
    14281 ttagcgaccg cccataatgg caatgtaatc ctgcgtccag cgactgtagg gaacaaactt
    14341 gcccccttct gctataggcg ttttccagaa ggctatttta tcgaaatagt taaaaccgtt
    14401 tgtttcacaa cctttctcgc ctaataacga actggcttta cacccttccg gcacgactgg
    14461 taacgagcca aaccaggccg ccacatcgcc ctgcactttt ggcgttaatg accagttcat
    14521 ccatttgtag gcgcaaaccg gatgtttcgc ttcgctatgt agcatggtgg tgtcagccca
    14581 accggtaacg ccctccttcg ggaaaacggt agcgacaggc tgaccttcgg ctttcagggc
    14641 gttggtctga tagggccagg cactggaagc gaccacaccc tcgtttttga aatcgctcat
    14701 ttgcacggta gtgtcatgcc agtagcgatg gatcaaacta tgttgatcac gcagcacttt
    14761 cagcaccgcc tggtactgtt cttcggtgag ttgatacgga tcgctgatgc ccaactgcgg
    14821 ctgagtggct ttaacgaaca acgcagcgtc cgcaatgtag atagggccat cataagcctg
    14881 aacgcggcct ttattgctct tgccgtcctg cagattttgc tcaacaaaaa ccacttgcca
    14941 gctatccggc ggcgtcggga aggttttagt gttgtacatc agcaggttcg gcccccattg
    15001 gtaaggtgtg ccgtaaactt tgccgccaac gttaaaccag tcgcctttaa ccacgcgcgg
    15061 atcaagcgtt ttccagttgg gaatcaaggc ggtattaatc ggctgcacgc gtttacccat
    15121 aatcaaacgc agcgaggcat cgccggatgc cgtaaccaga tcgtaacccc ctttggtcat
    15181 cagactgacc atctcatcgg aagtcgcggc ggttttaaca ttcaccgcgc agcctgtctc
    15241 tttttcgaat tgcgttaccc agtcgtattg tttatcagtt tgtccgcgtt cgatatatcc
    15301 cggccaggcg ataatatcca gtcgcccttc cggtttatct aaattggtag gcggttcggc
    15361 ggcgtgagcg gtcattattg tcatgctgag cgcacacagg ctgctgcggg caaatgtctt
    15421 gctcataagg tcttactcct gtcgtaatta attgttcagc cgccgtgccg taaaaatatc
    15481 tccttgttta accatagacg gcggcttgca ggcacacctt cgcccctgag aaaatttaat
    15541 caggttgtga gctaccgcgc attatgccgt ctgtaaggtt atcttctgga gtatagacaa
    15601 ggagttagga aatatgactg ctattcgaaa ttcatattac ccaatctaag aggaattaaa
    15661 ttaaacgcta ttacagccgt tcttgaataa gtttgcctaa ttgttttacc gcctgttctt
    15721 cacgttctcc ccacagccac gcggtattaa aacgaaaaaa acgtgaccag ttttcaccgg
    15781 tagaaaacat tttacctggc gcaatactga tatgatgcgt cagtgccgcc aggctcaatt
    15841 cgccggcatc taacggctcg gggagctcca gccagagaaa ataaccgctg tcattatgat
    15901 gaattttcac ttccgcaggc agataacgca gcagtgcctg ccaggcacgt tgtttacgtt
    15961 ccgcaagctg gcgacgcagg cgacgaagat gggcgtcgta tcggcgcgtg gaaaggtaat
    16021 ccaccagcgc aagctgcatc ggtgagctgg tggaaagcgt actcatcagc tgcaagcgtt
    16081 gaattttacg tgcatgtttt ccggcggcga cccaaccaat acgaaaacca ggcaccagac
    16141 atttcgaaaa cgaagagcaa tgcaaaacgc catcgtggcg atcccacgct ttcgcaggca
    16201 gcggtttttc ccgtccaaaa taaagttcgc tgtaaacgtc atcttcaatc agcgttacgt
    16261 tgtactgatt gagcaacgcc accagttgtg cttttttttg cggcgttaag gtaaatccga
    16321 gtggattctg gctattagtc atcagccagc acgctttcac cggatactcc tgcaacgcca
    16381 gttccagcgc ctgaagatcg atcccttctt taacatccgt cgccaccgat aacgccttca
    16441 gccgtagccg ctccagcgcc tgcaacgcac cgtagaaaca aggattctct actatcaccc
    16501 aatcgcccgg ttcagttacc gcttgcaaac tgaggtttaa tgcctctaac gccccggcag
    16561 tgatgacaat ttcatcagga gaaatggtga tcccctgtaa ggcatagcga cgggcaatag
    16621 cctgacgcag ttctgcgttt cctggcggca agttttcaat cacgctcatc gccgtggcgg
    16681 ttttgcttac ctgcgccagc gagcggttta gttgttggag ggggaaaagt cgcgggtcgg
    16741 gaaaggccga ggcaaacgga acgaccgacg gatcgcgact ggcctgcaac atatcaaaaa
    16801 tataagtgtt gatatcgact gcttcatctc gagtgactgg aatgactggc actttcggca
    16861 tttttattgc ctgtggcgca acgtaataac ccgactgcgg tcgtgcgata atatatccct
    16921 gactttcgag caactgatag gcatggctga cagtcataaa gctcatgcca gaaagcgcca
    16981 cctggtcacg caacgaaggt aaacgatcgc cgggttgcca gatacccgac gcaatctgct
    17041 cgcgtaattg ttctgcaagc tgctggtatt ttttcattat cgaatcgtaa ttatgtgcag
    17101 atgattcggc agtctatatc agttgttaaa aatgacaact tttcgttaac tgtaattaaa
    17161 ccatcaccag cgataactct ttgccaagcg cctttgccgc gagctggacg gcgtcgattt
    17221 ttgtcgcatg atgcaagtta aataggcgag taatctcttg tttaggtttg ccaattcgcc
    17281 tggctaactc ttgctgagtg atttctgact gtaaaaaagc atttaacagc aataccttag
    17341 aggcgacgct caaaggtact tcaataaagt gatcatgact atttaatggc gaaggtaaag
    17401 ggataagctc gttatcttca aaataaaaat caaatgcggt cagtaaagca tcttttgccg
    17461 cttccatcgc ttcagcgaca gtttcgccct gcgtcaacgc ttcagggata tccacaaaag
    17521 aaaccatata accgccttcc ggcgcgggtg taagagtgac gggataacgc ataatttcga
    17581 ctgtctctcg catgtcatta acctttttaa ttggcgatta actcaaaccg agttgtttca
    17641 ggattgcttt acgcaatggt tctttaatct catcgcaggg gtgacgcggc atgacactgc
    17701 gcctcccatg aaacctgagt ttcaaatggt tgctgccatt cgctacatcg acgccctgag
    17761 attcgagcca acgtctgaac tcgctttgtt tcacatcgcc tccctgcggc tgagtatatc
    17821 aacaaagtaa acattagtgt ttacctccgt gtgtaatttg ctcgaaaata gcactaaatc
    17881 gccgcaacgg cagaatgcaa tgcaaaatca ggaaggaaga ttccagaatt tctttgcgct
    17941 aatttgcaaa actgaataat aacctcaaga aacgatcggc atcatagcgg caaaaggtaa
    18001 gaaaaaggtg gcaggatgtt tacgaaggcg ttatcggttg tcttattaac gtgtgcgctg
    18061 ttctcaggac aactcatggc agggcacaaa ggacatgaat ttgtgtgggt aaagaatgtg
    18121 gatcatcagc tgcgtcatga agcggacagc gatgaattgc gtgctgtggc ggaagagtcg
    18181 gcggaaggtt tgcgcgagca tttttactgg caaaaatcgc gcaaaccaga agcgggacaa
    18241 cgttgagtcg tttgatgccg gatgcggcgt aacgccttac ccggcaggca atatcatgca
    18301 gattcaataa gttgtgagta accagaaaac tggcgtttta cccttttcgc ttcggcaatg
    18361 ttttcagcag ctcatccgga cttacggatg ccactacgct tcccggtaac ggcattttca
    18421 gtatgtgatt tttgattttg ccaatgacgt gcatctcgca tgggcggcaa tcaaacttta
    18481 gcgttaatac ttcatcgccg ttaaccagtt gcattggcgt cgccttccag cttttgatat
    18541 tgcctttcgc ctgtttcggg cacagattaa aggcaaagcg cagacaatgc ttggtgatca
    18601 tcaccgggac ttcgcccttc tcttcatgtg cctcatacgc cgcgtcaatc agctgcacac
    18661 catagcgatg ataaaattca cgcgctttct ggttgtaaac gttcgcgagg aaactcagat
    18721 gtgtttgcgg ataaaccggc gcaggatcag caaccggttt acggctgccg cgctggtaac
    18781 tggcaagacg cgcagcatcc agcatgtcgg cagcttcacg gcggaactgg tttaacagac
    18841 tgtttggtac aaacagcgcc cccggcaaat taatttgcac atcgcgggcg taatagaggg
    18901 tttgccccag ttttgccaga ccatccttca gattgttcat tgctttttct gcgttattgg
    18961 cttcgtcgaa ctgcccatcc agcgtatgcg ttatgctgac cccctcttca ctggtgagag
    19021 tcagaatcaa ttgttcctgc caaccgccca gttcaatgtc taccgccacc cgacgttcgc
    19081 tagaggtttt tgtcagtgcc tgctgccagt tatgatcaag attacggttt agtgggtgat
    19141 gcgggcgaat tttgtgcaaa tctgctggca tttcattggg ccagacgcgg tactgatttt
    19201 ctccggtttt ctcgaccgta ttggcacgaa aaccgacgac ttcacgttta atcaacacgt
    19261 tcaggccatc gccatttgcc agtggctcgg taacggcaac atcgagatga tctttcgcca
    19321 ctttcactac ttcacctacc ggcaggccga taaatttcgg cgaatcgaac gcgccaatat
    19381 cgcctttacg ggcattcaca aaataatctg tgctaccacg gtggaaagtc ttttccgtcg
    19441 atggaacaaa gaaatgttca gtacgacctg atgaagcgcg cgccagatcg ccacgttctt
    19501 caataatggc atcaagcatc tggcgataat gggcggtgat attcttcacg tagctcatat
    19561 ctttgtaacg cccttcaatc ttgaaggaac gtacgccagc atcaatcagc gcgccgaggt
    19621 tggcagtctg atcgttatct ttcatcgaca gcagatgttt ttcataggaa accacccgcc
    19681 cctgatcgtc tttcaatgtg tatggcaaac ggcacgcttg cgagcaatcg ccacggttgg
    19741 cgctacgccc tgtttgcgca tgagaaatgt agcactgacc cgaataggcc acgcacagtg
    19801 cgccatgaat aaagaattca atggtcgcgt ccgtggcctg gtgaatcgcg cggatctgat
    19861 caagattcag ctctcgcgcc agcacaatct gcgtgaagcc aacatcagag aggaacttcg
    19921 ctttttctac tgtacgaatg tcgcactgcg tactggcgtg cagttcaatc ggcggaatat
    19981 caagttccag aatccccata tcctgaacaa tcagcgcatc gacaccggtc tggtagaggt
    20041 cagtaatcag ccgttgcgcg ggttccagct catcatcatg caaaatggtg ttaagcgtga
    20101 cgaaaatttt tgcaccataa cgatgggcaa acggcaccag ctcggcaata tctttcaggc
    20161 tattactggc attatgacgg gcaccaaaac cagggccgcc gatataaaca gcatcggcac
    20221 cgtgcaaaat agcttcgcgg gcaatggcgg catcgcgtgc cgggcttaac agttcaagtc
    20281 gatgagaaga tacggtcaca ttcttatcca gtagctgaaa aatggcggct attttagcca
    20341 caagcgcgac gttgcgaaat agttttcccg cttaactgcg cgggtaatgg atgagggaat
    20401 gaaaatgtac ggtttgctcc ccaccattac gatagatatg cgtgacgtct gcagcaaatc
    20461 gaacgccttc cccacaatta agggtctgcc attcaccatc aacgcacaga tcaagttgtc
    20521 catcaatgac gacgacatgt tcgattaccc ccttctcatg gggcgtcgat tcgcttattg
    20581 caccgggtgc catctgaatc gagaagtgtt caaagcagag ttgtggatca taaggaaaca
    20641 gcgaaatgat caccatcgcc tgctgttgtg gatcataaac tgaaggcgta gcggactgtg
    20701 gcggagaaat aaatgtcgaa aatggcacgt tcaacccagt ggcaattttc cataatgtcg
    20761 cgaccgtcgg gctggactca ttacgctcaa tctgtcctaa cattgcttta gaaacgcccg
    20821 tcgcttctgc cagtcgcgaa agactccagc cgcgctgctg acgtagttgt ttaagtgtgg
    20881 tggataaaaa gcgagcgaga ttttccatgc gactcctccg gcaaaacgga agtttatcac
    20941 ttgtgcgtta taacggacaa atgctacggt gcctgtacgc tataacgcac gaggtgacta
    21001 tgcgtctgtt ttctgttcct ccacccacgc tactggcggg gtttctggcg gtattaattg
    21061 gctacgccag ttcagcggca ataatctggc aagcagcgat tgtcgccgga gccaccactg
    21121 cacaaatctc tggctggatg acggcgctgg ggctggcaat gggcgtcagt acgctgactc
    21181 tgacattatg gtatcgcgta cctgttctca ccgcatggtc aacgcctggc gcggctttgt
    21241 tggtcaccgg attgcaggga ctaacactta acgaagccat cggcgttttt attgtcacca
    21301 acgcgttaat agtcctctgc ggcataacgg gactctttgc tcgtctgatg cgcattattc
    21361 cgcactcgct tgcggcagca atgcttgccg ggattttatt acgctttggt ttacaggcgt
    21421 ttgccagtct ggacggtcaa tttacgttgt gtggaagtat gttgctggta tggctggcaa
    21481 ccaaggccgt tgcgccgcgc tatgcggtaa ttgccgcgat gattattggg atcgtgatcg
    21541 ttatcgcgca aggtgacgtt gtcacaactg atgttgtctt taaacccgtt ctccccactt
    21601 atattacccc tgatttttcg tttgctcaca gcctgagcgt tgcactcccc cctttttctg
    21661 gtgacgatgg catcgcaaaa cgcaccgggt atcgcagcaa tgaaagcagc cgggtattcg
    21721 gctcctgttt cgccgttgat tgtgtttact ggattgctgg cactggtttt ttcccctttc
    21781 ggcgtttatt ccgtcggtat tgcggcaatc accgcggcta tttgccaaag cccggaagcg
    21841 catccggata aagatcaacg ttggctggca gctgccgttg caggcatttt ctatttgctc
    21901 gcaggtctgt ttggtagtgc cattaccggg atgatggctg ccctgcccgt aagttggatc
    21961 cagatgctgg caggtctggc gctgttgagt accattggcg gcagtttgta tcaggcgctg
    22021 cataatgagc gtgagcgcga cgcggcggtg gtggcatttc tggtaacggc aagtggattg
    22081 acgctggtcg ggattggttc tgcgttttgg ggattaattg ccggaggcgt ttgttacgtg
    22141 gtgttgaatt taatcgctga cagaaaccga taataaaaag cgccatctaa cgatggcgcg
    22201 tctggaacta ctttttgtta acgtcaaaca tggtggcatc ggtcgccatg tcatcaataa
    22261 cttgtttaat attttcgaaa gccattggcg tactttcgtt attcaggtct ttaccttcgc
    22321 cctgacgaac gactttgata accggtttat tagtcgctgc atcaatcagc tcaccttcaa
    22381 aatacaagcg agtatccatg gtacggtggc ctgtagccat ttgcgtcccc gcaaccacta
    22441 atgcaacagg aaccacttca tagaattgca gcccttcttt gctggtatct acaccggtaa
    22501 tggccccacg gaaaatcaga ctacgcggcc ccgcggtggt aaccagtggt ttacgctgcg
    22561 ctatcgcttc tttcatttcg gtgttggtat agttcaaaat tttatccaga actttctgcc
    22621 ctacctgggt cgaaggtttc ggtaccggat aataagtgat tgggttccag acgatgctgt
    22681 catatttgct ttgatcaaaa ctcgggtcta cccaacgtaa aaccggttta cctgtagccg
    22741 aggttgtttc ttttaaatca gagtaattgt ttaaaaaacc agaatattta tctggctggg
    22801 tgattttaga ggcacaacca gacagagcca acaagccgca taaagctgca actttcgcaa
    22861 atgatgtggt acgcatgaat agtttcctga gatgattata ttacttttta agttatagca
    22921 aaaagcgccg cttcgtgttg tggcaaaaaa agtgttggtg aaaataaatt tttttagccc
    22981 cttttcagac gacatagtca atgccgcaaa aggggctaag tcaatcatca gtagttaaat
    23041 ttcagtaagt taagctgcta aaaaacttcg actactgtga ttaatttcac atcccttcga
    23101 taaaaaatat gcttaccgaa aagaaggaca gtaaaccatc tcagcgtggg gaattttcac
    23161 gcgctttgtt cttaccgggt cattttttac gtgccagcat cgtggcgaaa cgcagtttaa
    23221 tacgattacc gttggcgtcg gtgcggtgca gctcgccgac gtcttcattg tatttcacca
    23281 tctcccagcc ttcgtaatat cgacgtaatt ctccctcttt gaaggcaaac gggaagccga
    23341 cggtacatgg ataatcagcg gtatccatcg ccgccacaat caggttgtaa ccgccaggtt
    23401 tagtgcaacg ttgcatattg gcaatcagcc ctgggatggt tttagcctca aggaacatca
    23461 gcaccacagt cgaaagaata aaatcgtact ctccatcaaa tgtgaggtta ttcagatcaa
    23521 cgacccgggt gtgtaaatta tccagatttt caatggattt aatgcgctcg acgttggcga
    23581 tactcatggc atttttatcc catgcgtcaa catcataacc attggctgcc aggtaaagac
    23641 tgttacgacc attgccacag cccagatcca gcgttttacc cggtttaacc actttcaccg
    23701 cttccagtac ttcagagtgt gtgcgggtta attcatattt atcagtaaaa taattttcgt
    23761 cacgaatgat cattctttgt cctctgcttt cattaaaacg gcgcgctcgg ttctgactaa
    23821 caattttccc tgcatcagaa gcgccaaagt acggatgagc agtattgcaa taataaaatt
    23881 ggtaaagata aacagcggca ccgccagcgt atggaaaaat ccattatcgc tgccactccc
    23941 cagatgcaaa ccggtggttg ccagtgcaga tacgccgaac gagaaactcc agaatgaagc
    24001 attaaatggc tgggagagat accatggcat cagacgtagc ataaacagca gttgcagcag
    24061 tccataaccg aaaagcattt tcgccagcgt gtcaccctcg ccgccgttga cgctcagcca
    24121 ggcactacag gccaccagcg caggagcgag ctgaatgccg agtgatgtcc gcagtgccgt
    24181 gggtaattct cccgaactac gcagacgctg caagatcacc ggttcaaggc ttagccatga
    24241 gaaaacgcct gcgcctaaaa acaccagacc ggcgtcggtg tagcccaacg caccacaggc
    24301 cattgcgctg ataaagttgt tggcaactgt cggcagatac agtccaggcg tagtagcttc
    24361 ttcagggtga gatccgcgcc ataatcccgc agtttgccag gcggcataag ccaactgaac
    24421 cacgacacca aaactgaaca ggcacaccgc cagtgggcga aaccacggaa caaaaccaat
    24481 cgccaccagc atcgtcgttg caggaaacaa actcacaaaa ctgctcagca ctggatggcg
    24541 aacttccgcc agcacgctat gcggaaagcg tatgagtcgg gtaataaatg cgctagtcaa
    24601 taatccccag atgatcatcg ccagaatcac cagcccatcc cctaaccagt ggctgacctg
    24661 ccaaacctgg ctggcatagc gccaggcaaa tcccatccct atcgtcccca acacaatacc
    24721 aaagtagcct gccggcaaat tgagcacttt atcgctctgc atcttcttac tcattttgtt
    24781 taatttataa agtatatttg aaatgcattt tatggaattt tctcgagcat agccagagcc
    24841 gcagaatttg ctacggttat agtaagtcac ctgtgggaaa acgctatgcg caaatatcgt
    24901 ctaagtgaag aacagcgagc ctttagttat caggaagatg gcactaaaaa aaatgtgtta
    24961 ttacggcaga ttatcgccat tagcgatttt aacgatgtga tagcaggaac cgctggcggc
    25021 tggatcgatc gcgaaacggt gttagcacag gaaggaaatt gctggattta cgaccagaat
    25081 gctattgcgt tcggcggaac ggtgatttcc ggtaatacgc gcatcaccgg caccagcgta
    25141 ctgtggggag aggtttacgc aacggataat gtctggatcg ataatagtga aatcaaccag
    25201 ggtgcatata taagtgacag tgtcaccatt catgactcac tggtttgcgg gcaatgtaga
    25261 atatttggtc acgccttaat tgatcaacat tctatgatcg tcgccgcaca aggcttaacg
    25321 cccgaccatc aactcctgct ccaaatttat gatcgcgcaa gggttagcgc ctcgcgtatt
    25381 gtccaccagg cacaaattta tggcgatgcc gtcatccggt atgcttttat tgaacatcgc
    25441 gccgaagttt ttgattttgc cagtattgaa ggtaatgaag agaacaacgt ctggctatgc
    25501 gattgcgcta aagtctacgg tcacgcgcag gtgaaagctg gcatagaaga agatgccatt
    25561 ccaacgattc attacagttc gcaggtggcg gaatatgcca ttgtggaagg taattgtgtg
    25621 ttaaaacatc atgtcttgat tggcggaaat gccgtggtac gtggcggacc gattctgctc
    25681 gacgagcacg ttgtaataca aggtgagagc cgtattacag gtgcggtgat aattgaaaac
    25741 catgtggagt taaccgacca cgccgtcgtt gaagcctttg atggcgacac ggttcatgtc
    25801 cgtggtccaa aagtcattaa cggcgaggag cgcattacgc gaacgccgct ggcagggtta
    25861 ttatgaatcg ataatacgcg cgtataagtt cacatcatca taggcatcat tcaggaactc
    25921 agcctgtttc aggcaacctt caaggataaa accattgcgc aaagcgacct ggttgctttg
    25981 cggattgtcc acccgacatt tgatcacgaa gcgtctaagt tcaccagact gagcgtaatg
    26041 atgaatcagt gcctgcagcg cctgagaaat gatcccctgc ccctgatgag attcgtccag
    26101 tcagtagccg atttcagcgg ttttattcag tggttcaata cgattaaacg agataacgcc
    26161 gataagttca tcttctttga aaatcatgaa cattttggca tagccgcgtt gatgcaacat
    26221 cacattaccc tgcaccgttt ttcgcgtgtc ctcttcaatc tgaacgaact gcggccagtt
    26281 tagcgactgc tgtaaccagg ttttattttt acagattaac tgataaagag gagtgacgtg
    26341 attttctgca acagcatgta attcaagtga ttcgcttact tttatcgttt cagtcatctt
    26401 atctccagtc aatccatgca gttgttaaaa aagccgcctg gctggcggct taggaaagtt
    26461 tacacctctt tgacgctctc atgctgcagg attttgagca tctcatcttt taactgtagc
    26521 ttttgttttt tcatgcggac cacttccgca ttgtaccctc gatcgtcgga accttccttt
    26581 ctggcaattt catgatcaag tttattgtgt ttatcgaaca aggacataaa gcgaggattt
    26641 tcgtttttca gacgggatat taaatctcga tattctggaa acatacgcac tccctgttag
    26701 tggctgacaa ggtgtatctg aaatacacac catcagatta ctcatttaac ttagggaaaa
    26761 atgcgagcgg gatcgtgttt aaccagacgg cacctcccga agaaggtgcc gaagaaaaaa
    26821 cgattaactc atcacgcggt cgtcaacata ctgacgttta tccggcgctg gcgggaaata
    26881 ctgatacagc catgtttcac tgatagcgtc gccctgacaa cgcagataca tccgcatatc
    26941 gaccggatca gtggagtccg aagtcggata ccagtcaaac tgaatacgat aaccatcaat
    27001 gggttcaata tagagaattt cgatttgctt cgcttcccca ctggaaagcg taatcaccgg
    27061 ctcaatgcct tttggtgcgg cagctttcag atcaccacca acgaaatcga cggcaaaacg
    27121 acgcgcccat ttttcgggat agtgttcacc tggtgcccaa ccttccggga aaccgcccat
    27181 gccggtacgc gtcgccataa cgcgcgctaa tgggcaatga acaggcggtt gcgcactcca
    27241 gtacagacga tactggaatg caaactcatc acccgccttt acagcttttt ctggctgcca
    27301 gaagcagaca atgttatcca gcgtttcgcc cgttgttggg atttccatca ggccgatggt
    27361 gcccttaccc cacttgttac gcggttccac ccacagactt gggcgtttgt tataccagcc
    27421 cataatgtcc tgataatggg agaaatcacg atccagttgc aataaaccaa accctttcgg
    27481 gttgttgtcg gtgtaagcat tgaactgcaa tttttgcgga ttattcagcg gacggcaaat
    27541 ccactcgccg ttgccccgcc acatggacag acgatcagag tcatgaattt gcggatgaat
    27601 ggtgtcgcac atccgacgtt cattagtacc gcagctgaac atactggtca tcggcgcaat
    27661 gcccagctgt ttaatgtctt tgcgcgcata caggtgattt tccacatcca taatcacctg
    27721 acttttctca caatggatag tgaacttata ggcaccagta atgctggcgc tatcgagcaa
    27781 cgcataaacg gtaaaggtag ttgcccccgg ttttaccgta tcaaaccaga aggcggtaaa
    27841 gtcggggaac tcttctttac tgtcggtgta agtgtcaatc gccaggccgc gggccgacaa
    27901 accgtattga tatgtgtcat caacggcgcg gaaataactc gctccgagga atgatactac
    27961 atcacggcgc gccagttcgg gggctttaaa cacgcgaaaa ccggcaaagc cgagatcgct
    28021 ttgcccttct aattgtttgg tatcaacacc tgcatcgttg tatttgaaca actccgggcg
    28081 aaagtgaatt tcacgcgcca gatgtgttgc tggatctaca gaaaacatac gaacgcggcg
    28141 acggaatccc attcccatat ggaagaactg agcgtccagt tgacggttct caacgttatg
    28201 ccagagcgat ttttcggcgt cgtattgaat actgttataa gcctgcggcg tcattgtcgc
    28261 cagcgtgtca ggtaacggac gaggcgcacc acgccacgct gtttgcgcta agtcgtgcgc
    28321 cattgactgt agaatggaga agtcaaaacg ctgggtttgc ccgtcggcaa tatcagaatc
    28381 tgccgcgaat gccgcctgag aaaaaagaga agcaatgccg ctggtaccgc acacggcggc
    28441 catagccatt gaacctttaa taaatcgtct acgatccata cctgaaagtg agtccttctg
    28501 gtgttgtgaa tggtcccgcg ctggtcgttt tcagcggcaa gaaagtgctt atttatgacc
    28561 gctcactcta gacaatattt attaataatc caattgttga actgagagaa tatgcataaa
    28621 tcggcaattt ttttatgtcg atgggatgag cctgattcgt ttgtaaaatc aggcaaattt
    28681 ttgcaaaggc ggatggtcag aatttaaagc aaaccacagc gacgtttact gcgttcttcc
    28741 gcttcctgat aaagctggaa ctcatccaaa accggacaac ccaacgcctg ctcaaatgct
    28801 tcgcgactgg cattaccgtg gctgcgtgcc tgcgtttcat tacccagatt tgactggaag
    28861 atccccgccg cgctaacggg caagaaatct tcataggtaa tgggttgcgc cgcgacccaa
    28921 ccacgttcaa ttaagggctg tggatcgtct ccgggatgaa tcgcctgacg atgcgcctca
    28981 cctgaaggcg tcagacggta ccggaaccat gccagccctt gctgacgcat taaaaactca
    29041 ctgtcaggaa aagtgcggaa ggtttcctgt aaatgcattt ggtgagtgag attatcctgc
    29101 ccggttccag cgttacgcag aagatcatca tacagttgtc gacctttcgg cgttaatgcc
    29161 acgccacgct gctcaatttc accaaagcgc gcagtatgcg tgccctgttt ctgccccgca
    29221 aacaacaccg tctcttccag tgctttaaag ctggtctggc gtagtaaaat cggtacctcg
    29281 cggcgcggcg gcccttcgat cagaattttt ggttcaattc cgcattcagg catcatcgac
    29341 tgcacccggt caatatccag cgtacgtggc gtcagatggt tgatatggca tccaggaaaa
    29401 cagaccacat cagcaatcaa ccgatgttcg ttgtgcaagg cgcgataggt ttcttcatct
    29461 accgttgctg actggtgcca acggaacgtt tccagggcct cctgcacaaa ctcctgtgcc
    29521 tgtgtttcgt taaaaccgcc ccgctggtca tactcctcta acagttgtcg acaacgtggg
    29581 gtgaagatat cgcgctgacg cagaatctcc gccgctttct ggcgcaaaat ttcgttctcg
    29641 ataagctcaa ggcggagtaa ggaggtaaaa acgcggaagg gattacgcgc cagagaagca
    29701 tcatcaatgg gccgaaatgc tgtcgaatgt accggcaccc ctgcctgcga gagatcgtaa
    29761 tagctcaccg ggtacatccc cataatggca aacatccgcc gaagagtagc aagctcttgt
    29821 gcagtcccaa cgcgaatcgc cccatgacgt tcaacattca gtcgcgccag ctcgtctgca
    29881 tttaccattt tttcgtgcag ttgaggattg ttttccagca cagccagatt cacatcagct
    29941 accagttcca gcagcgtgcc atattgcgga acttcttgct ggtacatggc tgacattgcc
    30001 tgcgaaaact gttcccgaat ctcatccgcc gtgatgctgt tcgccatagt gtctgcctcc
    30061 agtggtcagt aatctggagt gtaggtaacc gcattcactc tggcgggaag aatttacaaa
    30121 ctgtgatctc gccgcgaaaa catcaatatt atccattttg ctgtaacata attgctttaa
    30181 ttgttaataa tattttgcaa tcaagttatc ataatcaaac aacttcactt gtcagcgaca
    30241 ccgcttcgtt tttaacatcg cttatggaaa aaaatagtct gtttagtcag cgcatccgtt
    30301 tgcgccacct tcatacattc gtagctgtcg cacaacaagg aactttgggg cgcgcggctg
    30361 aaacccttaa tttgagtcaa cctgcgctct ctaagacatt aaatgaactg gagcagctga
    30421 cgggcgctcg cttgtttgag cgtggtcgtc agggggcgca acttacctta cccggcgaac
    30481 aatttttaac gcatgcagtc agagtacttg acgccatcaa cactgccgga cagtcgcttc
    30541 atcgtaaaga aggtcctaat aatgatgtcg tcagggttgg tgcactacct actgcggcac
    30601 tggggatatt accttcggtt ataggtcagt ttcatcagca acaaaaagag acgaccttgc
    30661 aagttgcgac aatgagtaac cctatgattc tggcgggttt gaaaaccggg gaaatcgata
    30721 tcggcattgg tcggatgtca gatcctgaac tgatgaccgg gcttaattac gaactgctgt
    30781 ttcttgaatc gctgaagctg gttgtccgcc ctaatcatcc gctacttcag gagaacgtaa
    30841 cgctaagccg ggtgctggaa tggccggtcg ttgtatcacc agaaggcact gcgccacgcc
    30901 agcattcaga tgcattagta cagagccagg gatgtaaaat tccttcgggt tgtatcgaaa
    30961 cgctgtctgc ttcgctatct cgtcaactta cggttgaata cgactacgtg tggtttgtcc
    31021 cttctggcgc ggtaaaagac gacctgcgtc atgccacgct ggtggccctg cctgttccgg
    31081 gacatggtgc aggcgaaccg attggaatac tgacccgcgt agatgcgacg ttctcttctg
    31141 gttgccagtt gatgattaac gctattcgaa aatcaatgcc gttctgaaag gtgaagggat
    31201 ctgtcgatcc ctcctttaac attttcacac cggagcgaaa ctaactggtt cacccgctcc
    31261 gcgaggttct gccaacacag attgtttgtt cagacggaat acgtccaccg cctcagttaa
    31321 tcgtgccgcc tgttcttcaa gggacaccgc cgccgctgag gcctcttcta ccagagaagc
    31381 attctgttgc gtcaccttat ccatttcaga aatcgcctgg ctaacctgcg ttatgcccct
    31441 actttgctca tccgaggcgg cggcaatttc ctgcatgata tgtgtgacac tcgcgacggc
    31501 atcaacaata gtgctcatgg ttttcccggc cgttgccacc tcatccgatc ccaggtcaat
    31561 taacctgact gattcactga tcaagccttc aatttctttt gccgcctggg cgctgcggct
    31621 tgcgagtgtc cgtacttcgc tggcgacaac ggcaaatcca cgcccttgct cacccgctcg
    31681 cgcagcttca acggcagcat tcagtgccag aatattcgtc tggaaagcaa tactgttgat
    31741 gacggcggtg atctcagaaa ttttcttcga actcgtggag atagcgccca tcgtttttac
    31801 tacaccggaa accgtctgcc cgccatcgct ggctttaata gaagcctctt gcgccagttt
    31861 gctggcataa tgcgcgttat ccgcattctg tttcaccgtc gcagtgagtt gctccatgct
    31921 ggcggcagtt tgttcgatag ccgccgcttg ttcttcggtg cgagatgaca ggtccgcatt
    31981 gccagctgaa atttcgctgg tgccacgata aatctcttcc gcaccctgtc gaacagtccc
    32041 tacggtcatc cccagtgaat gctgcatttg ctgtaaatga cgacttaagc gaccgatttc
    32101 attacgaccc gccggttcat ccttcatcgt cagatcgcca ctagcgattt tttcaatccg
    32161 ttgtgcggca tgttgcagtg gacgaatgac gatccgacgt agcaccataa atgttatcag
    32221 cgtcatgacc agggcaagca caaacgcacc aatcatgaac atcccgccca ggcgggtgcg
    32281 ctgatgggcc agttccgcca gttggttggc tctggtgcta cgtatcttaa cggctttgtt
    32341 caaaatatcg gtataagcat tatccagcgt tcggatctgc tcactttcat gattgataat
    32401 cgcttcaaac atgccatttt tggcatattt caacataggt tgcatacccg tgatataagc
    32461 ctgaaagcgt tgatttaatt cagtgtcgag ggcttcatca gcaggtgttt tcaccggccg
    32521 attctgataa gcacgataac cttgctgcga ctgtttaatc tccgattcgg cttgcgcaat
    32581 atttcgcttc attgcttcca tttccgcaat acgactcgca gccccggcct gaatcatatt
    32641 gatccgggct gaacgcaaaa aatctgaact gttcgctaac ccggtccgaa tctcaatctc
    32701 cgcagtaaca tcacgctgat cgcgatcggc ctgccagagg aaatagccag ccagggctga
    32761 acttaatgcg aatagaacta agataccgcc tagaatgcag gcaaataacg gaaccaacct
    32821 gatgtgatgc aaaaaaccta atcgctgtga gggagttgta ttcattaccg ttgtctctcg
    32881 tccaggttta ctcctgatag aaagtcatcg gcatttgacg gtaattactt acgactaaaa
    32941 gcctctttgc gtcacatttt tacaacaatt attgcagaga agattaatgc cattggaatg
    33001 aagagagtaa cgaatggtta aactggctaa cccgaatgac gaaaaattct ggaaaacagc
    33061 acgcaatttc gtcaggtgat tattttcgtc cctaaggaaa ccacatagga tgctcacggg
    33121 tgcctgagac tttctgtttt caggctaacc gggaatcaga aagacgaagg ccccgaccga
    33181 tatttctatc aaccgaggcc aacgaatgaa ccaagcaatt cagatgttag cctcttaccc
    33241 gccatccggc aaggagaaag gctatgaagc gcaaccctct ggtggtgtgt ctgctcatta
    33301 tctgcattac gattctgaca ttcacactcc tgacccgaca aacgctctac gaactgcggt
    33361 tccgggacag tgataaggag gttgctgcgc tcatggcctg cacgtccagg taagggcaag
    33421 cgcggggatt ttccccgcgc atctgactag cagattcatt ttctcatggc acccctttta
    33481 cacttcagtt agtgcatgtt tttttcgatg cactaataga accatcatta cagacaaatt
    33541 tgccgcctgc cgtacagtgt gagatccccc ctttcgaacc agaacagggt ttgcgcccgg
    33601 catcggcata aaaggaaaca agcgctccca taaacaaaat aagtgccagt tttttcatat
    33661 aacctccatg gtttatcgaa tcttatgttc gatttgaacg ttatacgctg gtttattttg
    33721 ataccgttga tgagatcaaa gatcgggtga attgattaaa aataagccag atggacgtat
    33781 gggcaaggat tatggtttta tttgtcatac aaataaatat aataggcgct tccgatgtag
    33841 acccgtattc ttcgcctgta ccacgggtcg gttttagtac aggcgttttc tttaaatatc
    33901 cttcaggaac gtttacgcgg catcatgcgt agaagtgtgt tgtccttcca gaaatagtgg
    33961 tgtgccagtg cagccgcagc gtgcaaaccg atgacaaaat atcccagatt cgccagcgtt
    34021 tcatgccacg actttaagct atctacccgt tcgaaattgg cctctgaagc gtaaggcatc
    34081 gtcaaaccaa acgcaaacca cgggttgcct cggttataca tcatcaccaa accaatcact
    34141 ggcagcgcaa taaacaggag ataaatcacc aaatgtccca aatgcgccag tcccgtcatc
    34201 atcggttttg gcttaggtat aatcggcggg gttgggtatt tcagccttaa caaaagacga
    34261 acgaccatca gcacgagaat tgatatgccg caggaaacat gaatcatgtt gatgagtggc
    34321 cgatcgctac gtgggaagaa accccgaaac tccattgcgc aatacgctgc gataaccagc
    34381 aaaaagacca gccagtgaat gctgatttgt aaccttgagt atttattttc cataacattt
    34441 cctgctttaa cataattttc cgttaacata acgggctttt ctcaaaattt cattaaatat
    34501 tgttcaccct ttttcagtac gttacgtttc actgtaagaa tggagattgc ccatcccgcc
    34561 atcctggtct aagcctggaa aggatcaatt ttcatccgaa cgttcctgac aggagaaaac
    34621 catgagtaaa gttggtatta acggttttgg tcgtatcggt cgactggtgt tgcgtcgatt
    34681 acttgaagtc aaaagcaaca tagacgttgt cgctattaat gatcttactt ccccaaaaat
    34741 tctcgcctat ctgctgaaac atgattcaaa ctatggtccg ttcccctgga gcgttgattt
    34801 tacggaagat tcacttatcg ttgatgggaa aagtatcgcg gtttacgccg aaaaagaggc
    34861 taaaaatatt ccgtggaaag cgaaaggtgc agaaatcatt gtcgaatgta ctggctttta
    34921 tacctccgcc gagaaatcgc aggcgcatct tgatgctggc gcgaagaagg tgttgatttc
    34981 cgcccctgca ggtgaaatga aaactatcgt ttataaagtc aatgacgaca ctctggatgg
    35041 caacgacacc attgtttccg tggcatcatg cactactaac tgtcttgcgc cgatggccaa
    35101 agccttgcat gacagtttcg ggatagaagt cggcacgatg acgaccattc atgcctatac
    35161 tggcacccag tcactggtgg atggcccgcg tggtaaagat ttacgtgctt cacgcgccgc
    35221 ggcagaaaat atcattcccc acactacggg cgcggcaaaa gccattggtc tggtgatccc
    35281 ggaactgagc ggcaaactga aaggtcatgc gcaacgcgtg ccggtgaaaa caggttcggt
    35341 cactgaactg gtatcgattc tcggaaaaaa agtgactgcc gaagaggtga ataacgcact
    35401 taaacaagca accaccaata acgagtcatt tggttatacc gatgaagaaa tagtctcttc
    35461 cgatatcatt ggcagccatt tcggttcggt gtttgatgcc acgcaaacgg aaattaccgc
    35521 cgtgggcgat ttacaactgg tgaaaacggt cgcctggtac gacaacgaat atggcttcgt
    35581 cacgcagctt attcgcaccc tcgaaaaatt cgctaaactc tgacgcgcac aggcggagga
    35641 aaaaacctcc gcctctttca ctcattaaga ctgtaaataa accacctggg tctgcagata
    35701 ttcatgcagg ccatgtttac catctgcgcc gccaataccg gatttacgcc atccggcgtg
    35761 gaagccttgc atagcttcga agttttcacg gttgatgtaa gtttcaccaa acttcagccc
    35821 tttaatggct ttcatcgcga cgttcagatt ttgggtatag attgatgagg tcaggccgta
    35881 atcactgtca ttagccattg agatagcatc ttccagcgtg tcaaatgcga ctaccggcag
    35941 aaccgggcca aaggtttcct catgcataat cgacatttcc tggcgaacat ccagcagcaa
    36001 tgtcggcgga taataatatc ctttcccctc taccgctttg ccacccaacg ccactctcgc
    36061 cccttcttct actgcgcgcg ccactttttg ctcgaccctt tccagcgccg cggcgttaat
    36121 caacggcccc atcgcaatgt cgttgcgttc agcggggtta ccaaattgaa ccgcctgcat
    36181 cgcttcaccc agccgattga cgaactgatc ataaatgcct ttctgtacat aaacacgttc
    36241 tgcacagtta cacacttgcc cactattaat gacgcgtgaa tcaacgatgg ctttgactgc
    36301 cagttcaaga tcggcatcgt ccattacgat agctggtgct ttacccccca gttccaggca
    36361 cactttggtg atgtttttcg ccgcagtcgc catgatcttc tcacctgcag agacgctgcc
    36421 tgtcatactg accattgcga cctttgggtt acccgccagt tcttgcccaa cggtttcacc
    36481 acgccccaga acaaggttaa acacgccgcg cggaaggcct atttcatcga cgattttggc
    36541 gaatgcaatc gcattgtttg gcgtaaattc actgggttta atgacaatgg tattaccagt
    36601 caaaagagcg ggagccattt tgcgggcaat gaggaagaac gggaagttcc acggcagaat
    36661 gccggtagtc acaccaagcg cacgtttaaa cagaagaata ttttctcctg gacgatcgct
    36721 ttgaataatc tcgccctcgt aacgccgtgc ccactccgcc atgtaatcga tatagtcggc
    36781 agtaaaagcc acttcgactt cagccagctg ctggatcttg cccccttctt caacaatcag
    36841 cgcactgatt tcactggcgc gttcgcggat cccggcggag attttgcgca accaactggc
    36901 gcgttcaatt gcaggcaacg cttcccattc tggttgtgca cgttctgctg catcgattgc
    36961 cttacgggca tcctcggcct gaccatcggg aatgcgggaa atgacagcct ctgtagcagg
    37021 gtttaccaca tcaatccatg cgtctccacg ccaggtaaca aactgtccat caatatacat
    37081 aggatgttga acgggtactg acatgggcga ctcctgtgat ttatatgttt gtttttggca
    37141 aatacattgt taattaacat caatcatcat agagacggca tcatgaaagg acaattctgt
    37201 gaagggcttc ataaaaacaa gtgtaaaagg aataagaggc agaaataaaa ggcaggagtt
    37261 atgcccgttt gcaaacaagt ggttcacaaa cgggcaattt ttaacgtaac gagcgacttt
    37321 ccatcgcctc gatgagcacc gcatcgtgtt tcagcgtttg ccatgcatgg atgacttctg
    37381 ccggaaaatc aacgtgaacg ataaaatctc gcccgcgggg accgtagcca tcgctatcat
    37441 cgcgtaaacg cggcagctcg ccagtgagta gtgagagata acgctcaacc ggccataatc
    37501 cttgtaactg attaataaag attacactgt ctgcgttatt tcctaactga ggaacgaatc
    37561 cgggataact taaccagcgt ggtacatcgg gattgtcccc ggtcatacgg cggaacgtcg
    37621 ccatcgtgcg ccgctgcatg gtggggtcct gtacaacgat agctgtgtga actcgttcta
    37681 cggcctgatt caatagcgcg atgctaaagc gtgcgttttc accgcagttt gttgactggt
    37741 cttcaatcca gattttttca tgcggaatat gccagaactg atgagcgata tccgccagga
    37801 tggtcgcttc tgctctgcca gtggtgcgga tagtgttgta gtgcggatgc tgtgcgatgg
    37861 cgctatacaa aaaagtggtc gagtgaccga taccaccact aatcagtaaa ggaatttgct
    37921 gatcgcgagc aatcttacat gccgcatcga tagtcggcat aaccgcattt cctgcaagta
    37981 tgacgcaatc agcctgatac ggcacctcac cggagaaatc atcctgcgcc agccactgtc
    38041 cgataacatt tatggcatct atagttgccg tcgaaagcgt cggaaacggg gtaatgttca
    38101 tggttcctcc ttatcgttcc cttaagaata ctgcgtgaga taatagtgag aagcggagca
    38161 ctctgaaagg aagataatgc tttttctatc tcgccatcgc gaaccagaca ctggttttag
    38221 tcatacacta tttgcggaga aactgttgat aaaacatcaa taacatcatt tgacgtggca
    38281 ataacataat gttcgatcat tttattttta tgaatttcac aactttgcat agtaaaccat
    38341 gtaagataag tggaattggc tactctatag atccccgttt ggttaattcc ctgctttcct
    38401 gacataccta acaaatcacc ttcgtcagta acacgaaatg agtgtatcca gtcaaatgat
    38461 atcttaactt ctgacttgat cgccgggcat atcagggcca gttcaagcct ggcattctta
    38521 tagcttaaac ctgacagatc ggcgtgttta aaagacaacg aagtcggcgt gatatttata
    38581 aaacgttcat ccatgttaac acccttaata tatgagactg tactcaattt aaccttctac
    38641 aaaaatggtg tgagtctgtt taagtaccat gtataatcga taaccgttgc tgaattttaa
    38701 aattatcttg tccagttatt aaccattttt catgggaaac atctgcaata cattcattaa
    38761 tgcgaaatac aatttcccct caggcaccca tattctaatc ttatcatccc ctaatattat
    38821 aaattgcaat tgacgtttga aatctaaagt tttattttca ggtaattcag tttgtatttt
    38881 tttcttgata tcatcaaggt agatgtgatg acaatgttca ccaaacatac tcatcacttc
    38941 cagaccgttt acagatgtcc atctttgttc actttcccaa taaacaagaa ccggtttgcg
    39001 acaggtatat ctgcctaaaa agcgccgaat gtgttcttcc tgattctcgt aaaatattgc
    39061 ctctgcattc gagatttgcc gacgcattcg ccagcggatt ttttcttcat aaaaagacaa
    39121 gttgtcgaat ttgacattac gcacattcat tgtcaataac tcgcatggtt ttaaatgtaa
    39181 aacttaaaaa accaccaaac aaatgaagtg aaattttata cttctttgcc ttataaacaa
    39241 ttgtcgcatc tttaatatta cctgcatttt ggttatctat aagttcagca atatccataa
    39301 cctgcgataa atgttccact cctgtaaact cgaacctttt tcgttccctt ccggctgtaa
    39361 attcatcaac ataaagctcc gccgtcaata taaaattata ctcggtgtgg tgacattcca
    39421 ttctggtttc tactaaaaca ccatcatgcc agataatatt ttctacattt ttcatgctat
    39481 taactattct caggtaagta accattagag tttctaaact ggttcgatga cgcagatact
    39541 ctcgttaaca aacaatctag ctcaaatgtg cattgcgtaa ttatgatatc attattcttt
    39601 ttaataagtt cacgcaaaac acttttttga gtttcagtaa attcaataac atttattgag
    39661 cgtacaaaat cgctaatggt ctgttggcgt agaaattttt tctgtgacat gatgtaaaaa
    39721 ataaacttaa attcgtcgtc accgacgtta agtttaatcg gataaattat tgataaaata
    39781 tacagcgcgc gtcggtaatt ttcctctgga agtttttcat agaaagatat taaagcctga
    39841 ccaacgcgat caccctctat tatccgttca aattgcgacc tcagtaaaat atcatgtcta
    39901 ctcattgcaa tacgtcctgt tcgcccaagg cgaagtatga atgaaactta tgtaaaatag
    39961 taccgccctt gctatatttc agaattcata aagaaaaaac ccggcgctaa atgcttaccg
    40021 ggttttagat ttatcaggca aatagcaggg ttaaccgcta atctgctcca ttgcctgcaa
    40081 aatacgctta tctgaaatcg gataaggcgt accaagttgt tgagcgaagt aactaacgcg
    40141 cagctcttct atcatccaac ggatctcttt cacgtcttca tcctcacgac gtgcaggcgg
    40201 gagtttgttg aaccattgct gccacgcctg ctggacgttt tcgactttca gcatctgtgc
    40261 acgatcgcga tgtggatcaa ccgccagttt ttccagccgt ttttcaatcg cctgcaaata
    40321 acgcagcgtg tcgcccagcc gtttgaagcc gttaccagtg acaaaaccgc gatataccaa
    40381 cccgcccatc tgcgctttaa tgtcagaaag ccccagcgcc atggtcatat ccacccgccc
    40441 tttcagacgt ttgttgatat tgaacactgc cgtaaggatt tgctcgacct gcttcgcaat
    40501 atccaccacc gtgtcgttca gttcggcacg cactttttca tgcagcgcag caaagccttc
    40561 ttccgtccag accgggcctc cattggcgtc gatcagttga tccacaccgc atgagataca
    40621 gtcgtcgatc agctccagca ctttgccata cgggttaaag tacagcccca gcttggcttt
    40681 gtttggtaac ttctcatgta aatacttgat tggtgaggga atattcagca gcagtagacg
    40741 gcgaagaccg ttccacattg cctgtttttg ctccagcggg ttatcaaaca gtttgatcgc
    40801 cacactgtcg cgctcatcca ccagcgccgg ccacgccttc actttgtagt taccacgctt
    40861 ctgttcgtag ctttccggca actgaccaaa actccagata tgtaagccgc tctgctcgat
    40921 accgtcatcc gccaccgcag atagcgtttc ctgcactttg cctttcagcg catctttcag
    40981 atcttgtagc gagcgccctt cttttagctt cttgtttttg tcatccacca cgcggaaggt
    41041 gattttcagg tgatcgggca cctgatccca gtgccagtct tcgcggtcaa cggtaacgcc
    41101 ggtcatccgc cgtaactcgc gctcaaggct gtcgagcaac ggtaactcca gcggtgtgac
    41161 gcggcctaaa aacgcttcgg catagtttgg cgcaggtaca aaattacggc gtaccggttt
    41221 cggcaacgat ttaatcagag caatcaccag ttcgcggcgc agaccaggga tctgccattc
    41281 aaacccgctt tcctcaacct ggttaagtaa cggcagcgga atatgtacgg tcacaccgtc
    41341 agcatccgcg ccgggctcaa actgatagct caaacgcagc ttgagattgc cctgatgcca
    41401 gaagttcggg taatccagct tgctgatctt ttccgcccct tctttgatca acatgctctt
    41461 ttcaaagttg agcaaatcag gcgtttcgcg gctgactttt ttccaccagc tgtcgaagtg
    41521 acgagcggag attacatcgt ggccgatgcg ctggtcgtag aactcaaaca gcgtttcgtc
    41581 atcaaccaga atatcgcggc gacgtgattt gtgttcaagc tcttccactt cggcccgcag
    41641 tttcaggttt tcacggaaga atgcgtgacg cgtctgccag tcaccttcca ccagcgcgtg
    41701 gcgaataaag agttcacgac ataacgccgg atcgatctgg ctgtagttga ccttgcgcgc
    41761 ggcaacaatc ggcaaaccat aaacagtgac tttttccgtt gccatcaccg cgccctgcgc
    41821 ccgttcccag tgcggttcgc tgtaggtacg tttaatcaaa tgctgagcga caggctccac
    41881 ccattccggg tcgatacgcg cagcaatgcg cccccacagg cggctggttt ctaccagttc
    41941 cgccaccatt acccatttcg gcggtttttt gaataaacca gaaccgggga agatggagaa
    42001 acgcgcgtta cgtgcgccgg tatattcttg tttatcggca tctttcatgc cgatatggga
    42061 aagcaacccc gtcagcaacg caatgtgaat ttcacgatac tccgccggtt cgctgttcac
    42121 cggaatgccg agttctttca ccacctggcg caactgggtg tagatatcct gccattcgcg
    42181 cacacgcaga tagttgagat aatcggtacg acacaggcga cggaaggcgt tggaagaaag
    42241 cgccttttgc tgctcgccaa gataattcca cagattcaca aacgcgagga agtcagactc
    42301 tttgtcgtgg aagcgacgat gtttttcgtc cgatgcctgc tgtttgtcca tcggacgttc
    42361 gcgcggatcc tgaatggaga gcgcagacgt gataatcatc gcctcacgca cgcagccatg
    42421 tttttgcgct tccagcacca tacgcgccag acgtgggtcg acaggcaact gcgagagctg
    42481 gcgaccgagc ggcgtcagtt tataggcgct ggcctgttca tccgtggtga tcgcgcccag
    42541 ttcttcgagc agacgcacgc catcctggat attgcgttta tccggtgctt cgacaaacgg
    42601 gaacgcagcg atatcgccta gccccagcgc ggtcatctgc aaaataaccg aggccaggtt
    42661 ggtacgcaga atctccggat cggtaaactc cgggcgcgag aggaaatcgt cttcggaata
    42721 gagacgaata cagatccctt cggacacacg accacagcgg cctttacgct gattggcaga
    42781 cgcctgcgaa atgggctcaa tcggcaaacg ctgcactttg gtgcgatagc tgtagcggct
    42841 gatacgcgcc gtaccggggt cgataacgta tttaatcccc ggtacagtca gcgaggtttc
    42901 cgcgacgttg gtcgccagca caatgcgtcg cccgctgtgc gactggaaca cgcggttctg
    42961 ttcgctattc gaaagccgcg cataaagcgg caagatttcg gtatggcgta agttcagctt
    43021 gttcagcgca tcggcggtat cgcgaatttc ccgctcgccg ctcataaaga tcagaatgtc
    43081 gccggggctt tcctggctta gttcgtccac ggcatcgaaa attgcctgca actggtcgcg
    43141 ctcagtgtcg tcggcttctt caacaatcgg gcgatagcgc acttccaccg gataggtccg
    43201 accggagact tcaataatcg gcgcattatt aaagtggcgc gaaaagcgtt ccgggtcgat
    43261 agtcgcggaa gtgataatga tttttaggtc aggacgccgc ggcagcaact ctttcaaata
    43321 accgagcaga aaatcgatat tcaggctacg ttcgtgcgct tcgtcaataa tgatagtgtc
    43381 gtactgcatc agcaggcggt cttgctggat ctccgccagc aggataccgt cggtcattag
    43441 cttgaccatc gtgttatcac ttacgtgatc gctgaaacgc actttgtaac cgatgcaacc
    43501 gcccggctcc gttttcagct cttccgcaat acggttcgct accgttcttg ccgccaggcg
    43561 acgcggctgg gtatggccga tcagcccttt aatcccgcgc cccagctcca tacagatttt
    43621 cggtaactga gtcgttttac cagaacccgt ttccccggcg actatcacca cctggtgatc
    43681 acgaatcgct tcgagaatgt cctgtttttt ctgactaacc ggcaaattgt caggataagt
    43741 aatttccggt cgtgccgctt cacgcagcag gactttccct gccgcctggt caatctcttt
    43801 cgccatctcc tggaaaatgg cctgttgtgc atcaggattt ttaaccttct tcacgccgtg
    43861 cagacggcga gaaaaacgca gtctgtcacg cagcatcagc gaatccagcc gctgctgcaa
    43921 ggccgtaaaa gtcaattttt gttgttctgt cataacgtta gtgggcagta gtctgcctga
    43981 ttaattttct ttgaaatgat agatatagag taccacatcg cggctttcta tgcgttgttc
    44041 aataaattcg aacataggct tcgatttatt gcgctatctc tgtggcagga ttgtgaataa
    44101 agtgtcaaca agcaacgggg catcgcccat tcaaatatct ataaggaaac accatgagca
    44161 aggtattagt tcttaaatcc agcatcctgg cagggtactc tcagtctaat cagttgtccg
    44221 attattttgt tgaacaatgg cgcgaaaagc actccgctga tgaaatcacc gttcgcgacc
    44281 tggctgcaaa tccaattccg gtactggatg gcgaactggt tggcgctctg cgtccgagcg
    44341 atgcgccgct gactacgcgt cagcaggaag ctctggcact ttccgatgag ctgattgccg
    44401 agctgaaagc ccatgacgtt atcgttattg cggcaccgat gtataacttc aacatctcga
    44461 ctcagctgaa aaactatttt gacctggttg cacgtgcagg cgttactttc cgctataccg
    44521 agaatggtcc ggaaggtctg gtaacgggta aaaaagccat cgttattacc agccgcggtg
    44581 ggatccacaa agatggacca acggacctgg tgacgccgta tctgtccacg ttcctgggct
    44641 ttatcggcat taccgatgtg aaatttgtct tcgccgaagg gatcgcatac ggtccggaaa
    44701 tggcagcgaa agcgcagtct gacgcgaaag cagccatcga cagtattgtt gctgcataac
    44761 cggacacgat cttgatgtgg actgtagttc agtccacatc tcaatccact tacctgtttt
    44821 cccataattt caacatcgct ttgtgctctt ccttaagcac aatatgcgag cgtctggctc
    44881 gaataaagct aatcgcttca tcaactgttt tacagtgtcc gtaacataac agccatgccg
    44941 ccactaccag cgcactgcgc gataatccca atgcgcaatg gaccagaacg ctgccttgct
    45001 cttcgcgtaa tgtttccagc atcgccacgg cctgtcggag ctccccctct tccggaacca
    45061 ccagatccag catcggtaca cagaaataga gtcgatcttt tgtcgctcgt ccgcgaggga
    45121 attcaaaggt gacatccaga accgcattct gtgccggaat atgtcgtgga aacgccccta
    45181 aataaacacc agcagtcatt ttgctcaccg gctccaggcg acgacaaaac caacgcatag
    45241 atagccacat cccgatgcgc cagggcaatg tcagccagta aacggcgggc gggagtttcc
    45301 cctgactatc tttccctgtt gttatcgcgc caagcccacc gtacccacgg ccaataatga
    45361 gtagcgataa tactggccaa cataaccaga ctgaccacca taactgaacc atcatcatta
    45421 gctccatcaa cacaatgcac gcgcacgcgc ctacgacata tggcagtgct attttgattc
    45481 gacgttgatc aggtttctga taattccaac gacggtcgac gggtatcatc cagtcaatca
    45541 acatacctac cgccagccct gtgatgacat caataaaatg atgctgccag gttgtcagca
    45601 tcgaaatggc gatgagtaaa aaccatccgc cgcagacttt acgccacctc acagccagat
    45661 gctgacgaaa gtgacgccaa agtagccagc agagaacaat atgcagcgaa ggagactggt
    45721 tataaggcag atcaaacagt tcaagttgcg aaaatagcca tcctgtcacc ccactcactt
    45781 caggacggat aaaactaaac ttcagcgggt agagaaaaaa accgcagcag gccattaccg
    45841 ttgccagaat aagccggtgg acaagtcggc gctgttcgaa tgtcgagcta caaacgaaca
    45901 gcgaaaatcc atataaaaga tccagactcc agtaagggac aatagtccag ggaagaaaag
    45961 ggatcgccgt ttcccagccg aatacctgac tggggatatc atggctgtta aagtcctgaa
    46021 ccgcggtgaa ctgatttaga gatccatagg tgaagaagaa aaacggggcc agcaacaata
    46081 accagccagc gccttgtagc aatacgttcc ggcgttcagt tatcacgacg caccgccatc
    46141 gaaaccgtaa aaatgcccca ctcatcaatc cgttgtgtgc atttatcaaa tccggcatca
    46201 cgcacgagtg aatccatctc cccttgcgaa cgcacgcgca ttacccacgg tttaccatct
    46261 ttatgactgg ttaacacccc ggcaatcagc tccagttgtg ggtgccacgg ctgcccggtg
    46321 tagaggagaa tgccgcccgg ttcgatggca ttggcaagac ctgcgagtga gttttttacc
    46381 tgctcatttc cgggaaaaag ctcatacagt ccagagacaa tcgccagcgt aggccgcgga
    46441 gttaacgcgc tgagttcctc cgggttaaac gcatcgccct gttcaaaacg cacccgccca
    46501 gacattcccc gttgagcaat catctcttgc ccctgtgcaa catttaactc gctgtaatca
    46561 cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg catccagtac atagcgccca
    46621 tgccctgcgg caatgtcaac cacgcggacg gctaaacctt tggcgtggag atcggcaacg
    46681 gcttgtttaa tcagtatttg taaatgggtt ttgcgctggc gaataccgcg ccagccaaca
    46741 ctgttcaggt agattttgtc gattaagcgc ccgaatgcgt tactaccttg cggttgattt
    46801 tgatagacat aatccagcga actgcctgaa tcaaagccgg tgctcattcc gagatgcagg
    46861 cccgcagagt gcgtcccgaa caatttcatc gctttacgca taaagcgata cgccaaatca
    46921 accggcgata atggcacggg tccacctgaa aggagccgcc agcgatccgc tgatggtcca
    46981 gtgcggtctt catgctgata atcaaatttt tgtgacttgt tagcatataa acgactaata
    47041 aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt cacccaacgt gtcgtgataa
    47101 aagcctggca gcagatgcag ctctttcaga gggctacgta atctatgata aaaatcaatc
    47161 tgcggttggc gatgcaccac atagtcatcg cctgatatca gaagttgcgt ggggagcgta
    47221 atcgccgccg catcgctaac aatacgttca gacgttttat aaagatcgag caagatatta
    47281 acggcaatcg cccgtgtgat cagcggatca ttattgaaac tcgccacccg ttgccgatcg
    47341 tgggtcaaat agcgtccttt cacataggaa ttaataaaaa acagaccacg cagacgatgc
    47401 cataacgcca gcgcaggacg tgccagcggc acatacaatt taaccttaaa ggccggagaa
    47461 gccagcacca gcccgcgaat tgcaggcgca taatcatgaa cccatgtggc aaccagcact
    47521 gcgccgacgc tctgcgcgat cactaccacc tcttccagcc cgacctggct gtcgctggca
    47581 gcaaaacgga caaactcatc gacatcccgc actgaacgcg caagagatgg gctataacca
    47641 cggggccccg aagtttgtcc atgccctcgg gcatcccatg cataaaaagc agtatctggc
    47701 atcgccagtt catcaacgat atgttgtaga cgaccagaat gttcatgacc gcgatgaaat
    47761 aagacgatga cctttttcgc ccctggctgt aaagcgggcc agtgccgata aaacaacgct
    47821 gtattatcac tggtggtaaa aaaatgttcc ccagggatgc gtgaattttc cattttctct
    47881 attttccttc agtaacagca gtagcgaata aaataaaaaa ataccggagc ggtgaaaatt
    47941 aatgagtcaa tcctgtcaag gagtccgcca tgtcctggta atagttttcc gctatcttta
    48001 acacccacat ctcgtttgac ggctgacatc actacatcgc cgcaaaaacc actaatacca
    48061 attaacaaac ccgctaataa tgcctgtaat gtatttagcg gtgtcagcaa cggtccaata
    48121 atcagtgacg caatcatggt ggtgattacg ccccccacca gaccttccag cgtttttccc
    48181 gggctgactt tggggaccac ttttcttctg ccgcaggatt tcccccataa atactgtgca
    48241 atatcgtttg actctgttaa cgccaaaaga aataacacca gtaacgcccc gccctgggta
    48301 tttgtggttg tcaacattaa taaccaggcg gcatgactca aggcgaaaac ggtcattatc
    48361 cagccccaaa aaatggcgct cacggtatgc agaaaaccgg aggtatcacc tactaaaact
    48421 cgccaggtgg ttaatatcag aaacccagcc aaaggtataa ataacagaaa cagctcaaag
    48481 caactaaaac cgataagcaa atagttaaga ggaataaccc aatataacca acgcggaaaa
    48541 tgtacggata taagcgtaca atattctttc aatgccagaa aacttatcag cgcaaaaaac
    48601 gtcaacgtca tccagcgtgg acctgaaatc accagtgaaa aacaaatgac gataccccac
    48661 caggtacgaa tacgtagtgt aacttcatcc ccttttctct ccggtaaccg ccatacccga
    48721 aaacggttta tcagcgttgc taaaattaac aacgcgaaaa gtgttgccag agatttttcc
    48781 agcattatct ttcagcactc atcaggagca cactccgaca acgattaatc gcagtccaga
    48841 gaagcagaat tgaagcaatg ctccataaaa gattattcca ttgcatccac tgtggataaa
    48901 tggcaacggc cagcccccac aacccaaata ttaacgcgcg atcgcttttg ccaaaaggac
    48961 cgacataact acgaataccg ttaatcgtct gcgcgagtaa accgcaaaac tcagtcaata
    49021 tggtgcaaaa taacatgagt attacgagtg atgcgttact ttccggtaaa aataaaaacg
    49081 gtaaatagag cgcaatatcg gaaataacat cgccagtttc atttaaaatc gcccccagac
    49141 gtgtttgctg attgcactca cgcgccaaca tgccatccag cgcattgagt gccatacgga
    49201 taaaaagcac gatgggcaat agcaaaaaga ggatgggttg tgcgaccaac atcaacagca
    49261 atccggtaat aagagaaagc gccagtgcag caagagtgat gtgattcgct gtaacatggt
    49321 gcttataaag ccaaaacatc gtcggcctta acagcgactg aaagagcggt tttatctggt
    49381 agagcgtcat tatcaatcct tgataattcg gtttgcgcta tggtgcatca attcccctga
    49441 cggagatatc gcaacacgac gcttcattaa aaattcagaa tatatcaagg tattggggac
    49501 tcactgtcag gaatgattat ccccaataaa tcaagtggtt aaaaattatt acgctgttat
    49561 tcaatacaac tcatcaagtt ttctcatcgc ttgttcccgt attgcgcgcg tcttaccgtt
    49621 aagcgccagc tggcgaacta aatccagttc agggttaaca agctttaacg ccagcaaagg
    49681 atcacgtttc agcctcgcct ttaatacatc aactggaaaa cggggattat tcatcgccac
    49741 aatccaccac tcgggatcaa tttctgccgc cagtactgca ggcggtgtgt ggcggttgtt
    49801 aagtgcgccg aacttgaaaa atgtgagatt gcttttcgcc agtgtactcc accatatacg
    49861 atccggttgt tttgccagta catccagttg tgctaacgca tcggggtcta gtgcataata
    49921 aacacgtaac gcttcgttaa cctgcgcacg ggaactgttt tcctgcctca gaaaaaactt
    49981 cgcccaaata tcattgcgtg gcgtgttttg ctgtaattcc ttgagaacaa cagcgggtaa
    50041 attatcccaa ctaattaatg cactctctaa aaagtcccag tcgtccgaac gtatcagaga
    50101 ggtggcattt aatagcatct ggtcatcaac ttcaccgtag ctgataagct cagacaaaat
    50161 catatcagcg ctggtttcat atgcatctga atctgctttg ggattatttt tccgatgcga
    50221 taaagcacgt tgccaaattt tttgtttcat cgacgtgctc agatactgat ttttcgccag
    50281 attactccac ccctcaggat cgttgaaacg tgtcaataac cgttgtaaag cagcgtcaga
    50341 gtgaattatc tgcatatccc gacagcaaaa gtaggcaggg gattttttct ccatcctttt
    50401 aacaaaatta aactgtaagt tatctggcaa cgctaaaaat aacgcaccta ttagatcatc
    50461 gtcattctcg aatttttggt ataaatatac tgctattttg tcactctctt gtggcgtcat
    50521 ccgcaacaat tgaccatggt gtttcatctc tgccagcttt aacgctaagc ttgtgacaac
    50581 atcgtttgat gaatcgttcg ccaactgcat tgcctgattg taggtgagtt gtcctccagc
    50641 accgacctga cgaatagagg aatcatcact gcggatatag ccatcaataa tggattcggg
    50701 caataaccac gcatctttat aaagctcctg aatgatcata tcccaatctc ttttcgccag
    50761 agtcatgacg atatccagag ggagattttc acgtagcgca agattcaacg gaccgacacc
    50821 atcaggtaga tgactcattc gcgtcattat ttcgacagag agcggttcac ggtcgagcaa
    50881 ccccagaaca acaggctggt aacccaattg ctgatcggtc aactccagaa tctcattgtc
    50941 aggcatatat ctcgccatac tttcaatcgt ttgtccatca ttgctattaa taagttgttt
    51001 gcgtaggtta gctgaaattc ctttaagcct tgggagagtt cgcattatgg caagatcaaa
    51061 tgtcgcaata tctcgtcctt caaatgcagc aacgttgcct tcatccagca tttgcaattc
    51121 ctgcactaac tcaggagaag gcttaaggcg aaacagcgct tcttttttca tctgattaca
    51181 gacattccct gccatgcgaa tattgcttcg cttaaaaatt tctttcagag catcttcatc
    51241 atttattaga ccccaataat atgcatcagg atttattatc atcagcgctt ttttgacatt
    51301 ttcagtgtca tttttgtcac tcgcacggac catgaattca ctatttgtcg aagacgcaac
    51361 aacaggtgca attatgtcat tgataaatat cccacgcgca ttaatactcc ccttaatcgt
    51421 agacggatta tcgtaatatg agtcgataaa taccagtcca cttgcattta tcgaaccggt
    51481 aattaacagt ggtgaatccg taactatatt cgttgccgtc acgttaccga gaataaccca
    51541 tggattagaa agagagaaag tggaaatatt acctttgact gtcaaattgc caagaacaat
    51601 caatttttga ttactctcta tattaccttc taccactaaa tcgccacgat gcactttcca
    51661 gtccgatgca gataaaccga aggggacaag attcacgcta ctgttgatag cttgttcaac
    51721 ttgttgttta tcttcagcgg tagccgcaga aaaaaaagtt tcaatatcaa ctgcatttgc
    51781 aacgcatcga agttccgcct gtactggaag actcagcata aacaggggaa gtaaacctat
    51841 ccagctgcaa agcctgggct taaacatatg cactccatgc cattaaaaaa gaaacaaaag
    51901 ggaaatgcat ctttcccttt tgtatattcg ttattctcgc gaaataccat ccaacgtaga
    51961 caacactttc tcagaaagat gcaacttttc agcagccata ttctcccgta aatgcgcaac
    52021 agaagacgtc cctgggatca gcaaaatatt cggtgaacgt tgtaacagcc acgccagcgc
    52081 cacctgcatt ggtgttgcac ccaagctcgc agcaacatcg gaaagcgtgg aagattgcag
    52141 cggcgtaaag cccccgagcg gaaagaacgg cacgtaggca atgccgtcac gagccaaagc
    52201 ttcaatcatt gcgtcatcag cacgatgagc aatgttgtat tcgttctgca cacagacgat
    52261 ttcggcaatc ttgcgcgcct ctgcaacctg cgtcggtgtg acgttgctta ggccaatatg
    52321 ttttaccagg ccttgttgtt gcatctctgc cagcacggtc aggctggctt caatcgatcc
    52381 ttccgccggg ccatgaccat cccccatcat cacgcgcagg ttaaccacat ccagtacgtc
    52441 caggccgaga ttacgtagat tatcgtgcac cgctttttgc agctctgccg gagaaaatgc
    52501 gggcaaccag gatgcgtcct ctccacgccg cgcaccaatt ttagtgacaa ttgtcagatc
    52561 gtcagagtaa ggataaagcg cttcgcggat aatctgattg gtgacgtgcg gaccataaaa
    52621 gtcgctggta tcaatgtgat tgacgcccaa tgccagcgcc tcacgcagca cggttatagc
    52681 gacgtgacga tctcttgggg ggccaaaaac cccaggacct gccagttgca tcgcgccata
    52741 accaagacgg ttaacggatt ttgtaccgag agtaaatgta ttgctgctca tcgcaccctg
    52801 ccctgtaata aaacggatta agaaaaatca gtatagaaac gaaaagagat aaataatgcg
    52861 aaggcgtaaa caagatttct ggatgagtag cactattgtc ctgaaagaca ccaaaacatt
    52921 agacagcaaa agataagtca agcgtcgcat ccggcaataa aaaaccgcaa cctgtatcag
    52981 cgttgcggtt tgtgtatcac atgctaaaaa ttagaacgtt ttcttaaagt tcagcatcac
    53041 gcctttgtcg ctgatgccgt cctctttcca gtgatacgca tccagattca gcgaactttc
    53101 tttgctactg tacttcacgc cgacgctcgc caggctgttg ataccgccgc ccttctgacc
    53161 gtcatcgacg ttaaagtgct ggctgcctgc ccctgcaagg cttgccgtgc gctggctctt
    53221 gctgtagctc aggttcggcc cgccttccag cgtcgcattc gcgctccagc cgcctttccc
    53281 tgcgtagtcc agtttcagcc cgacgatgct gtccaccgcc gtttcgctgc cgctgttcat
    53341 gctcaggtta aagtctccgg cattgcgctc ctgatagccg ccttccagcg agtgacgcag
    53401 tttcactccg gcgtacgggg tgattttcag cccttcgcgc ggctcaaagg ttttcgcccc
    53461 ttcgctacgc aactccaggt actgctgttt cacatccgta tccgccgttt tgctgacgtc
    53521 gccgtaagcg accgagcggc tgctgtcgag attatgcacg tcataacgca gcgcgttgtt
    53581 ccaacgcata ccattgtcga aggacatctg gtgcttcagg ccaaagaact ggctgtagcc
    53641 gccggttacg ccattgtcgc ccgctttctg cgcaccgtcg ccatccagac gcgcgatacc
    53701 gtattccagg ctcatgctct ggctttcgct caggtcaacg gttttacgca gtgccagcat
    53761 gtcatactcg gtgttatttc cgagttccgc acgcgggtca cctttcgcca ccacgttaaa
    53821 ggccaggcca ttgcccacct tcggcgcggc atccgccagc atgctgaagc ggttgcttaa
    53881 cacacgcgct tcacggaata ccgtggtcgc ctggctaccg ctcacctgct tcagggcgct
    53941 attcagttca gcagtggtgc ccacgttcag gctggtatac agctcgttat tggtgtaacc
    54001 cgcatccagt gccttcgcca catcgttcac cgaagcatcg gtcgccacat cggtgtaggc
    54061 gtttttgctc atgatgacgt caacgttgcc gctggcatcg gtgctgcctt tggcggtcca
    54121 taccacggac gttgaggtga tggcatcggc gtcggtcagg ttgatgcctt ccaccacgtt
    54181 gtcgaaactg accgtggtgt ctgccgtacc ggcggcgaaa ccggtgttga tcccgacacc
    54241 attcatgctg gcgttgttga ccttcagttt accggcgctg ccgtcaacgt tggtaccgac
    54301 gatatagccg ttgaggttgt tactgctgcc atcgctacta ccgctatcag tcgtttcact
    54361 gaaaggtgaa gtggtgtaag tgtttggcat gtccggcaac gtgtagtcgg tgtaatgcac
    54421 ttcggtattg ttaccgctgt tgccgttcac cccttccaca ttcacgctgt cgccctgctt
    54481 aatcaacccc gaaccagtca cgccgtcggc aatcaccacc gtaccgttgt taacgatatg
    54541 accttctgtg cccagtacgc tgaacgcaaa cgagtcgtta gcaaaaatat tgatcgtgcc
    54601 gttgttctca attaccgcat cggcggtggc attggcatcc agttgcatac cagtcaatcc
    54661 ggtatcggtg gtgccttcgg taccgagatt gataacgccg ttgttaacca gctgtgcgtt
    54721 tttatcgcct tccatcgcca cggcgttgtt gccgctgatg ttcattacac catcgttgag
    54781 agcaacatag ttgttgtggg tgaatttaac cgcgctggcg ttgtctttat cgaagttaat
    54841 taccccgcca tcctggttcc acaagaaggc atcgctttct gtcgcaccgg cagtacttac
    54901 cgccacggtg actttcgcat ccaccacgcc cttgttaata aactcgctgg cgccatcgtc
    54961 gtagtgaata gcgcccgcac ctaatgcggc atccagcgtc atttcaccgg tgttatataa
    55021 cgtggcgtta gcattgctgc cctggaagcg aatggcgttt ttgctgttat caaggatgag
    55081 tttgccgctg ttgttgaacg ttgacttatc gtcaatatcc agcgtcccgg cattgttgtt
    55141 ttcatcctga taacccattt tccaggtacc cgcgttattc actaccgcag agttatagag
    55201 aatcgcgcct ttacgcgcct cgccgcctgc aaggttattg aataccgcat cgccctgagc
    55261 gttaacgcgc gccgacgcaa taccgctgtt attcaccgta ccatcgtgaa gttgtaaagt
    55321 tgacagggaa ccggtgttat taattaccgc gttgctgttt ttctcaattt ttacgacgtc
    55381 attaagcgtt ccgttgttat tgaaggttcc tccttccaga gtcaccgtgt tcaatacacc
    55441 ttcattggtt aacgcaccgc cttcattcac gacgatggct gagctaatgg ttccgctcga
    55501 ctcgttaacc agcgcaccat tttcgagtat cctgatcgtt ccatttttaa cagcacccgc
    55561 actggtgaca tccccgttga taatcgcacc tgacgataat aatacggtct cacccgcaga
    55621 acttaataca gcgccatcat catataaatc agcaatataa ttatccgttg gattatattc
    55681 atcggccccc tagcaaacgc caaaggtaca aattgtgcca tagttgataa taagcccagt
    55741 gccatcgtta tagaatgcct gaccattttc cgcgttaatg ttgattacgc ctgtctggtc
    55801 attaatggcg gtgccttcgt gataaaccgc catgccgacg agtttattca caccgagaga
    55861 atcgttgtaa ttttcatttt tctccaggtt gatggttccc tggttaatga ccgtattagt
    55921 attcccatac gccgccatcc ccgcaccggc gttgtagaca gtaatcacac cattctcgtg
    55981 gttggttgcc gtggctttag ttaatgcccc attataggca tcggtacctg aagccattcc
    56041 cacaccgaaa tctttcgccg tgctatttgc aacattatta cgcagcgatg tttcatcatt
    56101 ttcatcaacc cacagcgtat ccagggtgat ttgcccttcg ttatccgcgg ttgatttatc
    56161 gatggctagc atccctacac tgccacggcc atacatatca ataatacccg tttcgccgtt
    56221 cagaatggat gacgagtagc tggcaagcat acctgtcagc cctaatgcct tgttattgaa
    56281 agaggtacca tcagacaagt tccactctgc cggataattc agcacactgt gctgattcat
    56341 cattttggtg gtcatggtac cattgttaac gccaatccca ccgtattcaa catcaaagac
    56401 ttttggtgcc gggtcggtac caaaatcgta catcgccagg ccaatggatc cgttattgat
    56461 taccgttgaa tctttattcc aggcagccgc aaaaccgata tttttcagtt ccacttcacc
    56521 gttgtttgtc gccttcgcac cagaatcaat actaatggtg gataacgcta cacgataata
    56581 ataagtgaaa gaatccacat cactcatatc aaacgttgcg tcagcgccaa tctccagctc
    56641 cgcgccatgg ctcaactgaa tgagattttc gaaccccagc gggaactcgc cgctgatggt
    56701 tgactcacca ttcaggtaga ccggggaatg cccatcaacg ttaataagcg gtgtgtctcc
    56761 gataccacgt cgagaagctg atggttttga gccatcatcc atgacatttt tttcaccgac
    56821 aaaatggaca ccgccattaa gcgtaaaggt attgtcatcg ccagcaataa caatacctgc
    56881 aatgtgttcc tgagcaccgt cgtaactcgc ataggaacgc gacgtggttt tactatcagc
    56941 aacaacggtt aactgaccat ccagcgtgac gtcgttgttg tcaccgctga tatttacccc
    57001 ggttgaggga ttaaagaagt aaggctgcgc attatcggct ttctgatctt tatcaaccag
    57061 aatactgccc gctatttcga tagtattacc atcacccgaa acattaacac ccgtcgcttt
    57121 ttgccctgtc acattcagct cataagtcgc cagcgtcatg ttgttattat tgccagagac
    57181 atcaagaccg gtggagaaat ccccaacgtc taacccaccc gcgagtgaaa tattagcttt
    57241 atccccagca acgctgacac cggttccggc attggtcgcg gaaatatcac caacgttaac
    57301 aaatgtcgca ttatcaccgt cgatctgaat gccaatggca ttttcacccg ctgcggcctg
    57361 accaacattg aggtcgccca tgttgatcac gctgacgcca tcaccatcaa gcagcatgcc
    57421 aatagagtta tcttcgatga ccgtaatatt acctttgttg ctaacgaacg tatcattgcc
    57481 gtcaacgtca ataccgtgcg caccaccgct tacgtataag tcgccttcct gtttgatggt
    57541 ggcaccgtca ccgacaattt tcgagccagt ggaattctct ccgtcaacgc tcatggtacc
    57601 ttgattatcg acccgcgcat tatcgccatt gataaggcta cccgtaccac cattggtgat
    57661 ggtcgagtcg cccgcagtgt ttagcttggt attatcgcca ttgatgatta ccgcggtacc
    57721 gccatcaact tccgtctcgc cattattgtt aatggtgata ttgtcgccag tgagtatacc
    57781 gacaaccgag ccttctccga tggccgtcgt tttgccgtcg ttgttgaggg agatgttgtc
    57841 accatcaacc gtcatgacag taccgccatt cagcgccgtg ttgccgccct gatcggtgat
    57901 gatcgcgtta ccaaactcaa tcgcattccc ggttgtgccg tcaacaacga cgccggcctc
    57961 tttagtgatg tggatgagtc catcatcgtc gtaactccag gtgatgccac ctgaggtatt
    58021 tacgccatca aagacaacgg tgttgctgtc gtcatggact gtccaggact taactatcgt
    58081 ctccttgccc tcaggcgccg ttaaggtgta gctcccatcg gcattttggc tgtaggtaaa
    58141 ggtagtttcg cgaattttga cagtttttgc atcctgatcc caagttacgc catttttata
    58201 gacgacagga ttgtgtttaa ccggggtatc gccaccatcg tctggcggaa ctggcgtatc
    58261 accgccgtca tctggcggaa ccggcgtatc accgccgtca tcaggcggaa ctggcgtatc
    58321 accgccgtcg tctggcggaa caggcgtatc accgccatca tccggaactg gcgtatcacc
    58381 gccgtcgtct ggcggaacag gcgtatcacc gccatcatcc ggaactgggg agttattatg
    58441 gtggtgattg tcatcgtcac ttaaggtata tatagcaatt cccgtagcta ctgcagcaag
    58501 acctgcagct gcccatggta aaactgcact atctttattt tctctgcact ccgcggataa
    58561 attttctagc tgctctttgc tcagggaatg aatatttgcc ggacatgtta tcttactgga
    58621 cttacgtgtt ttttcggttg agtcgggtat tgaagtatcc gcagcccatg tctggctact
    58681 taacgccatg gctacacaaa cagacaataa ttttttcctt tgcatataaa attccttaga
    58741 tctagaccgt gcgtaacaag ccatgcagaa tttgaagata aatcgtagta aatattcata
    58801 gtgaaataag cagtaaagaa atgcttatat aattcacaaa atcaatatta aaatcaggca
    58861 agtaaaagca tacaaaaaaa acagttgatg gaattatatt ccacaaaaaa ctcacttcaa
    58921 tctaaaaatt aacgacaaaa aacaaaaaca cactaataat tcattgacgc tgcatatata
    58981 ttttgtgatt atatatatat tggtaatgat ttgttattta agggaaggat atataaggtt
    59041 tttgtagaaa tacccacggt aattaagcat gctaacgtca gtttaaatac tgtaaaagat
    59101 cattacatta atagatacat tttgtagcca actataattt atttgcgcaa ccatttacca
    59161 ttaactccct gaacatattg ccccgacttc gcgcgagcaa caagcttttg tccagccagt
    59221 ttggctatct ctttagtgga tacattattt tgctttgcca gttgttggta actatggtta
    59281 cgagcttcat taatatcttt tactaatgcc tgtgtttcgg catccgtttg tagcgccacc
    59341 aggtaaccgt taagcgtttc acctacccgc ccctgacttc tggcttcgtt cagggttaaa
    59401 gccattgcac tgtcgctcat caggccaatg gcccatacag ctatgagtaa tgttcttttc
    59461 atcatcacct caaaatagat cgctacgggt tttgagcagg tcttcaacgt ccttatccgc
    59521 tttaatgatg atttcatgct caattttaac gttcatatta atggtgattg gctctttcgg
    59581 cgcagccact tcaatgcgag gcgtgcaacc aaccaacata aaagaggacg caaacgcggc
    59641 tatcactatt ttcattgttt ttcctcacac tcttttcctg atgggcagcc agtttccggt
    59701 agctgggcat tctgctccag ccatacctgg agattatcgc caaagcgtaa actgcgccat
    59761 aacgtaaaaa tattctcttc atggtgataa ttaaggttta ccgtaccgct tttaccatcg
    59821 acgcgactgg tgcccgaaat gttggcctgc atggttaaca caccgagatt atctaaatta
    59881 atccttgttg atgagcgact gatttccata taacgcaacc agttgatggc agttccagcg
    59941 gtcatattgt ctttggccac cgcatctgca gtgtctttat ccagacgcag cgtcatcggc
    60001 cccgcgtttg ccaaccagcc atctttgaca atccattttt cattgttcag ccatagtggc
    60061 aacgccccac taaatgcccc agacatagca aactgtttcg gattaaccgc gctaaccagt
    60121 tcgctggatg agagattatt cagccgcaac agcgccgggt catgttgcgg catacgtaat
    60181 tgtttcatca gcacgttacc gcctaacaca tcgacgctaa catcagtcaa cagcaatggt
    60241 tcttcttcag tccacggata acgcccttgc aaatcagccg taatattttt cgctgtcacc
    60301 agattaatca cttcggcaat tcgcaacgta acggggccgc gagtccccag atgccaggct
    60361 ccatcggcaa aacggaaagg caggacaaaa tcgacaccgt taacctgatt atctggcatc
    60421 caggcactac cgcctttcaa cacgccgtgc cctcccgcgc ggaatccttg ttcaggcgca
    60481 gcagaaaatg caacctgagc atacagttca ccatcgcgta agttcatctt ccagtcgggt
    60541 ggcaccagcg gctggaatac agtcagtgat tgtttaggcc accaggcgtt accgcgcaga
    60601 cgaataccgt cccagcgacc atttacccga accgggccaa tctcaccagc atgtaaatcg
    60661 cctttaaaga ggaaatacgt cggatcgcgc ccatcgacgc taaattttaa ggttgatggc
    60721 ggtaacacac tgccgccagt gaacagcgtt tgcccggcgt ccagtgacag cgcgccgctg
    60781 aaggagggat gctgtgcgtc acgtacccag acgacgggct tgtcgagaat taatcgcggc
    60841 ttttctaccg tcatcgtacc gtattgtaac tggtcgaaac cggtggagag atcggtcagc
    60901 gtaatcgtgc tgtcatgcca ctcacctttt cctgcgacat cccaggtggc attcatcggt
    60961 gtaaaactcc cttttcccca gtagcgccac tgccagcggc cagcgtcagg gagaaaatca
    61021 ttcgccagcc catccatatg cagcacgaaa tcgcccagtt cattttcatg cgcctgcaaa
    61081 atggcctgca aacgtccgtc aacaccacgt tgggtgactt ttacacccgc taaaggccag
    61141 cggatttcat cgatgtccag cgaatcgatg actcttccct ttgaacgaag taacgcgcct
    61201 ggctcaaagg tcagcgttgg gtcagacaga cttccactta gctgcgcagg taaacgggca
    61261 tataaaatga ggtccgcctg tttcgcttca ccggtcagct gcagaggcag ctggctgtta
    61321 tccatgctta attttcctgg gccaaaatta agcaccgcgt tgcccttacc cgcttgcccc
    61381 tgggtcagca cactcagtcg tccgctgacc agagcgttct caagccctgc ctgccagttg
    61441 tcgactttga cacccagtcg gccactcaaa ggaaaacctg catacggcca gctccagcga
    61501 ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag caacggatcg
    61561 ccgttatccc gtgccagcac gatcaattgc ccgctatttt cctgccagtc cagctcggca
    61621 tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgccc acttacagga
    61681 agtccatccg gcatgagcgg catagtaaat tcccccacca gtttaaccga cggctgattt
    61741 tcaaacgcga cgacatccag ttcgctgact gtaagttgtt gccctttcag ctggccttga
    61801 aatttaactt tttcgccctg ataacgcagt tgctggatat cagaggttaa tgcgagagag
    61861 agttttccct gccattcctg ccagggagaa aaaatcagtt tatcaatatt gatccaggtg
    61921 ttaggcagca tggactgcca ttgcgcgagg gtttttggag cagctggcga ttgctccgtc
    61981 tgcggcaatt tcgccagaca agcagaatca agttctaccg tgccgacgtt caataaccag
    62041 cggctgggat gtgaaaggct ggcgttggtg atatgcgcaa gctgacaatc tcccaccaga
    62101 taacggagat cgggaatgat taaaccttta cgcgtaatgc gtgggctttc atctaatgca
    62161 atacgtgtcc cgagcggtag ccagatgccc gccagcgtgg gaacccacag cccgagcgtc
    62221 atcagcagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac ggctttatat
    62281 ttacccagca tgggtagtta atatcctgat ttagcgaaaa attaagcatt caatacgggt
    62341 attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat agttgttgat
    62401 gaatttttca atatcgccat agctttcaat taaatttgaa attttgtaaa atatttttag
    62461 taacttaaat gtgattcaac atcactggag aaagtcttat gaaactcgcc gtttatagca
    62521 caaaacagta cgacaagaag tacctgcaac aggtgaacga gtcctttggc tttgagctgg
    62581 aattttttga ctttctgctg acggaaaaaa ccgctaaaac tgccaatggc tgcgaagcgg
    62641 tatgtatttt cgtaaacgat gacggcagcc gcccggtgct ggaagagctg aaaaagcacg
    62701 gcgttaaata tatcgccctg cgctgtgccg gtttcaataa cgtcgacctt gacgcggcaa
    62761 aagaactggg gctgaaagta gtccgtgttc cagcctatga tccagaggcc gttgctgaac
    62821 acgccatcgg tatgatgatg acgctgaacc gccgtattca ccgcgcgtat cagcgtaccc
    62881 gtgacgctaa cttctctctg gaaggtctga ccggctttac tatgtatggc aaaacggcag
    62941 gcgttatcgg taccggtaaa atcggtgtgg cgatgctgcg cattctgaaa ggttttggta
    63001 tgcgtctgct ggcgttcgat ccgtatccaa gtgcagcggc gctggaactc ggtgtggagt
    63061 atgtcgatct gccaaccctg ttctctgaat cagacgttat ctctctgcac tgcccgctga
    63121 caccggaaaa ctaccatctg ttgaacgaag ccgccttcga tcagatgaaa aatggcgtga
    63181 tgatcgtcaa taccagtcgc ggtgcattga ttgattctca ggcagcaatt gaagcgctga
    63241 aaaatcagaa aattggttcg ttgggtatgg acgtgtatga gaacgaacgc gatctgttct
    63301 ttgaagataa atccaacgac gtgatccagg atgacgtatt ccgtcgcctg tctgcctgcc
    63361 acaacgtgct gtttaccggg caccaggcat tcctgacagc agaagctctg accagtattt
    63421 ctcagactac gctgcaaaac ttaagcaatc tggaaaaagg tgaaacctgc ccgaacgaac
    63481 tggtttaatc ttgccgctcc cctgcaatcc aggggagctg attcagataa tccccaatga
    63541 cctttcattc tctattctta aaatagtcct gagtcagaaa ctgtaattga gaaccacaat
    63601 gaagaaagta gccgcgtttg ttgcgctaag cctgctgatg gcgggatgtg taagtaatga
    63661 caaaattgct gttacgccag aacagctaca gcatcatcgc tttgtgctgg aaagcgtaaa
    63721 cggtaagccc gtgacctgcg ataaaaatcc gccagaaatc agctttggtg aaaaaatgat
    63781 gatttccggc agcatgtgta accgctttag cggtgaaggc aaactgtcta atggtgaact
    63841 gacagccaaa gggctggcaa tgacccgtat gatgtgcgct aacccgcagc ttaatgaact
    63901 cgataacacc attagcgaaa tgctgaaaga aggtgcacaa gtggatctga ccgcgaacca
    63961 gttaacgctg gcgaccgcga aacagacatt aacttataag ctggcggatt taatgaatta
    64021 atagctgcca cagctcccgg cggcaagtga ctgttcgcta cagcgtttgc cgttgggtaa
    64081 tgcacacatc ccaatcgccg taccatccag ttgacgggca acagaaagcg aaccgccgat
    64141 cattgcacaa tttgcttctc cactactgga catcgacgct tttaaacctg gcgctacgtg
    64201 cgccgcagtc gcctgctgaa caggctcact actacacgcc gacaacaata aagcggcaca
    64261 ccctacccaa aacgctgctc gcatctcttc ttcctctgat cttcaagcca aacgacaccg
    64321 ccataaataa taggcagcac agagggcgac gtcgagagct gtcctgcgcg ttgccccgca
    64381 tttttacttt tttatggcta tttttttgcc ctctgtttga tcaaaacatt cattacgctg
    64441 atgtggggga cacaaaagcg aaaatgcaga agaaagccat ttgctaaaat tgaaagatta
    64501 ctactgggcg cgcagcaatt tcgtgcgccc ctcatttgcg caatgtaagg gtgtcatatg
    64561 attactattg acggtaatgg cgcggttgct tcggtggcgt ttcgcaccag tgaagttatc
    64621 gccatctacc ctattacccc cagttccacg atggcagaac aggctgatgc ctgggccgga
    64681 aacggcttga agaacgtttg gggagacaca ccacgcgtgg ttgaaatgca gtcggaagcg
    64741 ggtgctatcg ctaccgtgca tggcgctttg cagacgggtg ccctttcaac atcgtttacg
    64801 tcatcgcagg gtttgctgct gatgatcccg acgctgtaca aactggcagg cgaactcaca
    64861 ccgtttgtcc tgcatgtagc ggcacgtacc gttgccacac atgcactctc tatttttggc
    64921 gatcattccg acgtcatggc ggtgcgccag acgggttgcg cgatgttgtg tgcggcaaac
    64981 gtccaggaag cgcaagactt tgctctcatt tcgcacatcg cgacgctgaa aagccgcgtg
    65041 ccatttattc atttctttga tggtttccgt acgtcccacg aaatcaataa aattgtcccg
    65101 ctggccgatg acactattct tgatctcatg ccgcaggccg aaattgatgc tcatcgcgcc
    65161 cgggcgctca acccggaaca tcccgtgatc cgcggtacgt cagccaatcc tgacacttat
    65221 ttccagtctc gcgaagccac caacccatgg tacaacgcgg tctatgacca tgttgaacag
    65281 gcgatgaatg atttctctgc cgcgacaggt cgtcagtatc agccgtttga atattacggg
    65341 catccgcaag cggaacgggt gattatcctg atgggctctg ccattggcac ctgtgaagaa
    65401 gtggttgatg aattgctaac ccgtggcgaa aaagttggcg tgctgaaagt tcgcctgtac
    65461 cgtcccttct ccgccaaaca cttgctgcaa gctctgccgg gatccgtacg cagcgtggcg
    65521 gtactggaca gaaccaaaga acccggtgcc caggcagaac cgctctatct ggatgtaatg
    65581 accgcactgg cagaagcctt taataatggc gagcgcgaaa ctctgccccg tgtcattggt
    65641 gggcgctatg gtctttcatc caaagaattt ggcccggact gtgtactggc ggtatttgcc
    65701 gagctcaacg cggctaaacc gaaagcgcgc tttacggttg gtatttacga tgatgtgacc
    65761 aatctgtcac tgccgttgcc ggaaaacacc ctgccaaact cggcgaaact ggaagccttg
    65821 ttttatggcc ttggtagtga tggcagcgtt tccgcgacca aaaacaatat caagattatc
    65881 ggtaattcca cgccgtggta cgcacagggc tattttgttt acgactccaa aaaggcgggc
    65941 ggcctgacgg tttctcacct tcgcgtgagc gaacagccga ttcgttccgc ttatctcatt
    66001 tcccaggctg attttgttgg ctgccaccag ttgcagttta tcgataaata tcagatggct
    66061 gagcgtttaa aacctggcgg cattttcctg ctcaacacgc cgtacagcgc agatgaagtg
    66121 tggtcgcgct tgccgcagga agttcaggcc gtgctaaatc agaaaaaagc gcgcttctat
    66181 gtgattaacg cggcgaaaat cgcccgcgaa tgtggcctgg cggcccgtat taataccgtc
    66241 atgcagatgg cttttttcca tctgacacaa attctgcctg gcgatagcgc cctcgcagaa
    66301 ttgcagggtg cgattgccaa aagttacagt agcaaaggtc aggatctggt ggaacgcaac
    66361 tggcaggctc tggcgctggc gcgtgaatcc gtagaagaag ttccgttgca accggtaaat
    66421 ccgcacagcg ccaatcgacc gccagtggtt tccgatgccg cccctgcttt cgtgaaaacc
    66481 gtaaccgctg cgatgctcgc cgggcttggt gatgccctcc ccgtttcggc gctgccgcca
    66541 gacggcacct ggccgatggg caccactcgc tgggaaaaac gcaatatcgc cgaagagatc
    66601 cccatctgga aagaggaact ctgtacccaa tgtaaccact gcgtcgccgc ttgcccacac
    66661 tcagctattc gcgcaaaagt ggtaccgcct gaagcgatgg aaaacgcccc tgccagcctg
    66721 cattcgctgg atgtgaaatc gcgtgatatg cgcgggcaga aatatgtctt gcaggtggct
    66781 ccggaagatt gcaccggttg taacctgtgc gtcgaagttt gcccggcgaa agaccgtcag
    66841 aatccagaga ttaaagccat caatatgatg tcgcgcctgg aacatgtcga agaagagaaa
    66901 atcaattacg atttcttcct caacctgcca gaaatcgacc gtagcaaact ggaacgtatt
    66961 gatattcgca catcgcagct gattacaccg ctgtttgaat attccggtgc ttgctccggt
    67021 tgtggcgaga cgccgtatat taaattgctg actcagctct atggcgaccg gatgttgatc
    67081 gctaacgcca ccggctgttc ttcaatttat ggcggtaacc tgccctctac accgtatacc
    67141 accgatgcca acggtcgtgg gccggcatgg gcgaactctc tatttgaaga taatgccgaa
    67201 tttggccttg gtttccgcct gacggtcgat caacaccgtg tccgcgtgct gcgtctgctg
    67261 gatcaatttg ccgataaaat cccggcggaa ttactgacgg cgttgaaatc agacgccacg
    67321 ccagaggttc gtcgtgaaca ggttgcagct ttacgccagc aactcaacga tgttgccgaa
    67381 gcacatgaac tgctgcgtga cgcagatgca ctggtggaaa aatcaatctg gctgattggt
    67441 ggcgacggct gggcttacga tatcggcttt ggcggtctgg atcatgtatt gagtttgacg
    67501 gaaaacgtca acattctggt gctggatacg caatgctatt ccaacaccgg tggtcaggcg
    67561 tcgaaagcga caccgctggg tgcagtaact aaatttggcg agcacggcaa acgtaaagcg
    67621 cgtaaagatc ttggcgtcag tatgatgatg tacggtcatg tttatgtggc tcagatttct
    67681 ctcggcgcgc agctgaacca gacggtgaaa gcgattcagg aagcggaagc gtatccggga
    67741 ccatcgctga tcattgctta tagcccgtgt gaagagcatg gttacgatct ggcactcagc
    67801 catgaccaga tgcgccaact cacagctacc ggcttctggc cgctatatcg cttcgatccg
    67861 cgtcgtgccg atgaaggcaa actgccgctg gcgctggatt cacgcccgcc gtcagaagca
    67921 ctggaagaaa cgttacttca cgagcaacgt ttccgtcgtc tgaattcgca gcagccagaa
    67981 gtggcagaac agttatggaa agatgctgca gctgatttgc aaaaacgcta tgacttcctg
    68041 gcacaaatgg ccggaaaagc ggaaaaaagc aacaccgatt aaatgctctg gataaggatt
    68101 atccaattct aaaaaaaagc ccggacgact gttcgggctt gtctttttat atgttggaaa
    68161 atcagtggca atgcaatggc ccaacagaaa ataaaatctg tgacaataaa ggcatataac
    68221 ccgcgcagaa taacgtatag aaaattaaaa gtataatttt tattttgtat gtattatctt
    68281 cgatacattc accagactta ttatcatttc agaggaatta tttgattaag gttttactta
    68341 aggcgtaaca aatgatattt atcggctaac tgaacttctc ctttaggatg ttttcactcc
    68401 ccgaaatggg gataaaaggc aaataaaata actaaaggat ttattcaatg aaaagcaaag
    68461 tactggcact tttaattcct gccctgctcg gcgcaggtgc tgcacatgca gccgaagttt
    68521 ataataaaga cggcaacaaa ttagatctgt atggcaaagt tgatggcctg cattattttt
    68581 ctgataattc agcgaaagat ggcgaccaga gctatgcgcg tctgggtttt aaaggcgaaa
    68641 cccaaattaa cgatcaactt actggctacg gtcaatggga atacaatatt caggcaaaca
    68701 acactgaatc ttcaaaaaac cagtcatgga cccgtctggc atttgccgga ctgaaatttt
    68761 cagattacgg ttctttcgat tacggacgta attatgggct ggaccgatat gctgcctgaa
    68821 tttggcggtg actcttatac caatgcagac aactttatga ctggtcgtgc caatggcgtc
    68881 gcgacttatc gtaatactga tttcttcggt ctggtaaatg gtctgaactt cgcggtgcag
    68941 tatcaaggta acaacgaagg tgccagtaat ggtcaggaag gcaccaacaa cggacgtgat
    69001 gttcgccatg aaaacggtga cggctggggt ctttccacaa catatgattt aggcatgggc
    69061 tttagcgctg gtgcggcata cacctcttct gaccgcacca atgaccaggt taaccatact
    69121 gcggcgggtg gtgataaagc agacgcgtgg actgctgggc taaaatacga tgctaacaat
    69181 atttacctgg caaccatgta ttcagaaacg cgtaatatga ccccgtttgg cgacagcgat
    69241 tatgctgttg caaacaaaac ccagaacttt gaagtcactg cacagtacca gtttgatttt
    69301 ggtctgcgtc cggcagtctc tttcctgatg tctaaaggcc gtgacctgca cgctgccggt
    69361 ggtgcagaca acccggcagg tgttgatgat aaagatctgg ttaaatacgc cgatgttggc
    69421 gcgacttact atttcaataa aaacatgtcc acttatgttg actataaaat caacctgttg
    69481 gatgaagatg acagtttcta cgctgccaac ggcatctcta ccgatgatat tgtcgcttta
    69541 ggtctggttt atcagttcta aatcctcctg cccgctgtta tggcgggctt tttctgctta
    69601 ttcttcctcc tttgatctaa attaaaaatg tgaactccgt cattacacaa aaagtgtcat
    69661 ctggcgttac actttatgcg gatactaaaa caggaggttt tatgaacaga acgattcttg
    69721 tccctatcga tatttccgat tcagaattaa ctcaacgcgt gattagccac gttgaggcag
    69781 aggcaaagat tgatgatgca gaggttcatt tcctgacggt aataccttca ctgccctact
    69841 atgcctctct gggtttagcg tattccgcag aattaccggc aatggatgac ctgaaagcgg
    69901 aagccaaatc gcaactggaa gagatcatta aaaaatttaa actgccaacc gacagagtgc
    69961 atgtccatgt tgaggaaggc tcgcccaaag accgcattct ggaattggcg aagaagatcc
    70021 ccgctcatat gatcatcatt gcttcccatc gaccggatat caccacttat ctgctcggtt
    70081 ccaacgccgc agctgtagtg cgtcacgcag agtgctccgt gctggttgtg cgctgacact
    70141 aacgcccgca cattgctgcg ggctttttga ttcatttcgc aaatgtgctg acattttccc
    70201 ctctaatccg taccatacac gccacagttt ttatatcaga tttcttatgc tgggcgttcc
    70261 ggcatgacgc atactcttct gatgccatat aacgaattga gtcgctttta aatgtcgcaa
    70321 aatcaagaaa ttagcaagaa agaacaatac aacctgaaca aattacaaaa acgcctgcgt
    70381 cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga tcgcatcatg
    70441 gtttgcctct ccgggggtaa agacagctat accatgctgg agattctgcg caatttgcag
    70501 caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc
    70561 ttcccggaac acgttctgcc cgagtatctt gaaaagctgg gcgttgagta caagattgtt
    70621 gaagagaaca cttacggtat cgtgaaagag aagattccag agggcaaaac cacttgctca
    70681 ctgtgttctc ggctacgtcg cggtatcctt tatcgcaccg caacggaact gggggcgacg
    70741 aagatcgcgt tgggccacca tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc
    70801 tacggcggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg caaacatatc
    70861 gttattcgtc cgctggccta ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa
    70921 gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca acgtcaggtg
    70981 attgctgaca tgttgcgtga ctgggataaa cgttatccag ggcgtatcga gacgatgttc
    71041 agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt cgatttcaaa
    71101 ggcatcaccc acggttctga agtggttaac gggggtgatc tggcgtttga tcgcgaagag
    71161 atcccactac aaccggcggg ctggcagcca gaagaagatg aaaatcagtt ggatgagtta
    71221 cggctgaatg tggttgaagt gaaataactc ggatagcgcc cgatgcgcaa acgtatcggg
    71281 ctactctaat ggaggctgaa taagacgcgg tcagcgtcgc atccgacaat cccgacgaag
    71341 atgtttactt ttgcatcagg caattcaaca ttttattatt ttaataaccg cacccggcac
    71401 gtttttcctt taatcttccc gccctgtaac tgtttccatg ctttatgagc aacagcctga
    71461 cggaccgcga catagacatg cgccggatgc acggcgattt tgccaatatc tgcgccatca
    71521 agcccgatat ctcctgtcag tgcacctaat acatcacccg ggcgcatttt ggcttttttc
    71581 ccgccatcga tacacaacgt tgccatttct gcttccagcg tcacaatgga actattagct
    71641 ggtggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt ggcccgctgt
    71701 gcttcttccg gagcacagaa actgatcgcc agaccgctat ttcctgcacg agctgtacga
    71761 ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac caccagctca
    71821 agcgatttaa tatccagacc acgcgcagca acatcagtcg cgaccagtac acgggcgcta
    71881 ccgttagcaa aacgtaccag ggtctgatca cgatcgcgtt gctccagatc gccgtgtaat
    71941 gacaatgcac tttgccctac ttcattcagc gcgtcgcaga cagcctggca atcttttttg
    72001 gtattgcaaa acaccacgca agaggatggc tgatgcaagc ttaataaccg ttgcaacaga
    72061 ggaattttgc ctttgctgga tgtctcataa aattgttgtt caatgggtgg caaagcatct
    72121 gttgagtcaa tttcaatcgc caaaggatcg cgttgcactc gtccgctgat tgcggcgatg
    72181 gcttccggcc aggttgccga aaacagaagc gtctgtcgag atgcaggcgc aaaacggatg
    72241 acatcatcaa tggcatcgct aaatcccata tccagcatgc ggtcggcctc atccatcacc
    72301 aaagtattca gcgcatccag tgataccgtg cctttttgca ggtgatccag caaacggccc
    72361 ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat cacgctgcat accgaacggt
    72421 tgaccaccgc acaacgtcaa aattttggta tttggcagaa aacgcgccag ccgacgcaat
    72481 tcacctgcca cctgatccgc cagttcacgc gtaggacaca gcactaaagc ctgggtttga
    72541 aatagcgacg catcaatttg ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg
    72601 ccagttttcg cctgcacgcg aacatctttt ccggcaagga tcgccggaag cgcggcggcc
    72661 tgcaccggcg tcatggttaa ataacccaac tcattaaggt tcgtgagttg ggcgggaggc
    72721 aaaacattca gggtagaaaa agcggtcaca atctattctc gtggtcatcg acgcaaagtc
    72781 atcaggcgcg tatcctcgca gatctacgct cacgatgcga caatttaatc ggttcttcat
    72841 cgggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggcact ggaacaggat
    72901 cgccaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc atatttccct
    72961 ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca aaaaaaagcc
    73021 gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc aaaaaaggaa
    73081 gtaagacaat atggagcgca acgcccatcg cttgacgttg cattcacctg caagagagat
    73141 attgccctga atgggtagag agtttattga cttcgctcaa actttgcggc gtttttgtat
    73201 acagacagcc ggaaaaattg cttttgttac aaccatttac tacgatgcaa ccataaagca
    73261 acaccaccaa taagaacaac taacagaata caaaaaattg aaaatccgaa ttgccacccg
    73321 ccgccaggga tcccaccaag gttgacgcca aataaccctg tcagaaaggt actgggtaaa
    73381 aagaccattg ccatcaatga cattgtatag gtacgacgag ctaaattttc ctgcatcacc
    73441 tgagcgattt catccgccat cacaccagtc cgtgctatac aggcgtcgat ttcgtcaagg
    73501 ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc gttggtcatc gctcatccac
    73561 ggcaaacgtt cactggcaag acgagcataa acatcacgtt gcggtgccat atagcgacgc
    73621 atcacaatta attgtttgcg cagcagagcc aggaatccac gcggcggaat ttgctgatca
    73681 aggagattat cttcaaggtc gataatttta tcgtgcagct gctcgataaa ttcgctggaa
    73741 tgatcggtca acgcatcgca cacatccacc agccatcccc cgcaatcggt cggacccgtg
    73801 ccctcttcca gatcactcac cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa
    73861 acaattaacc gcccgtccat atatacacgc atggcgacca gttgatcggg gcgttcatcg
    73921 gtgctgccgt ttatacagcg caatgtaatc agcgtgcctt caccgagacg gctgactcgg
    73981 ggccgtgtgc tctcgcccgc cagcgcatca cgtacgttat tgggaagcag cggtgttgtc
    74041 gccagccatt gggcgctatc atggtgtaca taattaaggt ggagccagca gggatgcgct
    74101 tcatcaatca catctgtatt ttccagcggt ttaacgccgc ctctaccatc cagcatccag
    74161 gcaaatactg catccgggac attaacgtcc gatcccttaa tcgcttccac agtgcctcca
    74221 tcatcaacgc attattttgt agtctagcct tctggccctg ttacgcaaca tctcatcacc
    74281 ccattaccct gaaatgatta ataaaattct gtctaaattg aatacaaaaa gcaaaatgct
    74341 tttccgtata caaaccgtgt gaagtgttaa atagcgtcta tcattatcag aattatctga
    74401 tcatatgacg tggctttttt gcgatcggat agcaacaaaa attgataaaa ataatgggat
    74461 ctcaatgatt acgcacaact tcaatactct ggacttactc accagtcctg tctggatcgt
    74521 ttcgcccttt gaggaacagt taatttatgc caacagcgcg gcgcgactgt tgatgcaaga
    74581 cctcacgttt agtcagctac gaaccggtcc ctattccgtc tcctcacaaa aagaactgcc
    74641 gaaatacctc tccgatctgc aaaaccaaca cgatattatc gaaatcctca ctgttcagcg
    74701 taaagaagag gaaacagcat taagctgtcg gcttgttttg cgagagctga cagaaacaga
    74761 accggtgatt attttcgaag gtatcgaagc gccggcaacg ctgggtttaa aagccagtcg
    74821 ctcggcaaat tatcagcgca aaaaacaagg tttttatgcg cgcttttttc tgactaactc
    74881 tgcaccaatg ttgttgattg acccgtcacg agatggacaa atcgtcgatg ctaacctcgc
    74941 cgcgctcaat ttctatggtt ataaccatga aacgatgtgc cagaaacata cctgggaaat
    75001 aaatatgctc gggcgtcgcg tcatgcctat catgcatgaa atctcgcatt tacccggtgg
    75061 tcataaacct ctgaatttta ttcataagct ggcggatggt tcgactcgtc atgtgcagac
    75121 ctatgccgga ccgattgaaa tttatggcga caagctcatg ttatgtattg tgcatgatat
    75181 tactgagcaa aaacggctgg aggagcagct ggaacatgct gctcaccatg acgcgatgac
    75241 cggattactg aatcggcgac agttttatca cattacggaa ccaggccaaa tgcagcacct
    75301 cgccatcgct caggattaca gcttattgct catcgacaca gatcgtttta aacacattaa
    75361 cgatctctat gggcattcta aaggtgatga ggtgttatgc gccctcgccc gcactctcga
    75421 aagttgcgct cgcaaaggcg atttggtgtt tcgttgggga ggcgaagagt ttgtcttatt
    75481 actaccaaga accccactgg ataccgcgct ttcgctggct gaaactatcc gcgtaagcgt
    75541 ggcaaaagtg agtatttcgg gcttaccacg ctttaccgtc agcattggtg tggcgcatca
    75601 cgaaggaaat gaaagcatcg atgaactgtt taaacgcgtt gatgatgctt tgtatcgggc
    75661 gaaaaatgat ggacgcaacc gcgtgctggc ggcataagcc acggatgcgt ctcgagatca
    75721 acgactgcgc ttagcgtggc gctcccagtt ttcttgcttt gcctgtgccg ttttacgtag
    75781 tgcgacgtaa cacgccccgc tgccgccatg atgcggtagc gcggtacaat atgcctggac
    75841 atcatcaaat tcggtcaacc agcgcgccac atagctgcgg acaatattgg catgcgattt
    75901 atcatcccgc cctttaccat gaataatcag cacgttacgc aaaccatccg ccatggcttg
    75961 ttgaatgaaa ctgaacatca ttttgcggca ctcttccacc ggctggcgca aaagattcag
    76021 gctcgcctgt tgcggatatt taccactgcg cagcttatcc agcaccccat gttgcaaccc
    76081 ttcccgccga aactccagcg gctgacttag cgggatgatg tcgagaaatc cggtggtgag
    76141 gaaattatca agttgcagcg tgtcgatacg ctgcggcgca cgttggttac gcgttggatg
    76201 ccagtggaca tcggtagcac gtttcagcgg ctggacatct tccatggcgt caagaaacag
    76261 cgatttgtcg tcaaggttca tgttacatcc tcccgcaatt aagagcgcga tatgataacc
    76321 agaccgggtc ggtccaacaa cgtattaccc aaaattccag taataagttc caaatattgc
    76381 cgatatttta agcaaaatgc ttatgcatga ttattcattc acgatattaa taatgtaact
    76441 tatattttcg tgaaatctgt cactgaagaa aattggcaac taaaggttaa aaccgttata
    76501 acacagtcac cggcgcagag gagacaatgc cggatttaag acgcggatgc actgctgtgt
    76561 gtactgtaga gtctggcgga tgtcgacaga ctctattttt ttatgcagtt ttaactttgc
    76621 agatagccgc attctcgccg gaattgcgtg attaatgacg cggtcagtgg tgtctggcga
    76681 ctatcacgcc gctgaatcaa ataataggcc gctttcggta aaatttcgct aaccggcaac
    76741 atcaccagcc cctgtccgtg caagggatcg cagcccattt cttcaggcag tatgctgaga
    76801 aaatcgcttt ttgccaccag actgatacag gctgagaacg tctcgcagac tacaccgacc
    76861 tgtggcgttt gcgcctgatc gtcaagcaat tcactcaact gtttgtagta gctgccgtgt
    76921 ggcgtcggca ttgtccagct gtaatccagt aactgtttga tcgaacgggc accaatggcg
    76981 gggtgtcccg ggcggcaaaa gatcgcgaat tgcttttcca gtaatttctc aaaagtaaat
    77041 tcgtggtcgt acggtccctg ataataggta ttgatggtga aatccaattc tccctgacgc
    77101 aattcattaa tcatcgacac cagttgccct tccataatgc gtacttttac ctgcggatgc
    77161 tgctgatgaa aacgagatat tacagctggc atcagactgc gggaaatact ggcccccatg
    77221 ccgatattaa tctgccctgc cagttgccct tgtcgttggc gaatatcctc ttgggctgcg
    77281 cgcagttctt caagaataag actggcgtgc tgataaaaac tttcaccggc atcagttaac
    77341 gtcacgcctt tactacggcg aaaaaagagt tgcgccgcta acccttcttc cagctcctga
    77401 atagatttac tcagtgccgg ttgcgacata ttcaacattc ggctcgctcc gcgaatgctg
    77461 ccctgacgag ccacttcaac aaaagcccga atttgatgaa tttttacctg aaaagccatg
    77521 acgccaccga taaccgttat ttatcagacc aaagaaactg gcatctactt taatgcagat
    77581 gattgtgtca gggtaattta tgaacggtta aaactgtgaa aaatcagtta gtgataagta
    77641 aaaactatcg ctacgtgaac cgggtcacac tttttactga tgacgggaaa ggttatggag
    77701 tctttgaatc aatttgttaa ttcgcttgcc ccaaaattat cgcactggcg acgtgatttt
    77761 catcactatg cagagtctgg ctgggtggaa ttccgcactg ccacccttgt tgcggaagaa
    77821 ttgcagcagc tcggctactc actggcgctg ggccgcgaag tagttaatga aaatagccgg
    77881 atgggactac ctgatgaact cactctacaa cgcgaattcg agcgcgctcg tcaacagggt
    77941 gcgctagcac aatggattgc ggcttttgaa ggtggtttca cgggtatcgt cgccaccctg
    78001 gataccggtc gccccggtcc ggtgatggct ttccgtgtcg atatggacgc gctggatctc
    78061 agtgaagagc aggatgtcag ccatcgcccc taccgcgacg gttttgcgtc atgtaacgcc
    78121 ggaatgatgc atgcctgtgg tcatgatggg cataccacca ttgggcttgg gctggcgcat
    78181 acccttaaac aattcgagtc cggactacat ggcgtcatca aactgatttt tcagcctgca
    78241 gaggaaggta cgcgtggcgc gcgggcgatg gtcgatgcag gtgtcgtaga tgatgttgat
    78301 tattttactg ccgtgcacat tggcactggc gtacctgcgg gcaccgtggt gtgcggcagt
    78361 gataatttta tggcaaccac caaatttgac gcgcacttca ccggtaccgc cgctcacgca
    78421 ggcgcaaaac cagaagacgg ttacaatgcc ttgttggcgg cagcacaagc cactcttgca
    78481 ctgcatgcaa tcgccccgca cagcgaagga gcttccagag taaacgtggg cgttatgcag
    78541 gcaggaagcg gtcgtaacgt tgttcctgcc tcggcgttgc tgaaagtgga aacacgcggg
    78601 gccagcgacg tcattaatca atatgttttt gaccgtgctc agcaagcgat tcagggcgca
    78661 gcaaccatgt atggtgtcgg cgttgaaact cgtctgatgg gtgcagctac cgccagttct
    78721 ccttcgccgc aatgggtcgc atggttgcaa agtcaggcgg ctcaggtcgc gggggtcaat
    78781 caggccattg aacgtgttga agcgcctgcg ggttccgaag atgccacatt aatgatggcc
    78841 cgcgtgcagc aacatcaagg gcaagcctcc tacgtggtgt ttggcacaca gctggcggca
    78901 ggtcatcaca acgaaaaatt cgattttgac gagcaggttc tcgctattgc cgtcgaaacg
    78961 ctggcgcgca ccgcgctcaa ttttccctgg acgcgaggta tctgatgcag gaaatctatc
    79021 gttttatcga cgatgcgatt gaagccgatc gccaacgtta taccgatatt gccgatcaaa
    79081 tctgggatca tccagaaaca cgttttgaag agttctggtc agcggagcat ctggcttcgg
    79141 cgctggaatc tgcaggcttc accgttaccc gcaacgtagg caatatccca aatgccttta
    79201 ttgcttcgtt tggtcaaggc aaaccggtta tcgccctgct gggagaatat gacgccctgg
    79261 caggtttaag tcagcaagca ggttgcgcgc aacctacatc cgtgacgccc ggtgaaaatg
    79321 gtcacggttg cggacacaat ttgctgggaa ccgccgcctt tgccgctgca atagccatca
    79381 agaaatggct ggaacaatat gggcaaggcg gcacagtgcg cttttatggt tgtcctggcg
    79441 aagaaggcgg ctcgggtaaa acgttcatgg tccgcgaggg ggtatttgat gatgtggatg
    79501 cggcactcac ctggcacccg gaagcctttg ccggtatgtt caatacccgt acgctggcaa
    79561 acattcaggc atcatggcgc tttaaaggga tcgcagcaca tgccgcgaat tcccctcatt
    79621 tgggacgcag cgcccttgat gccgtaacgt tgatgaccac tggcaccaac ttcctcaacg
    79681 aacatattat tgaaaaagcg cgcgtacact atgccatcac aaatagtggc gggatctcgc
    79741 ccaacgtggt ccaggcgcag gcagaagtgc tttatcttat ccgcgccccc gaaatgaccg
    79801 acgtgcagca tatttatgat cgggtcgcca aaatcgccga aggtgcggca ttgatgaccg
    79861 aaaccacggt tgaatgccgc ttcgacaaag cctgttccag ttatctcccg aatcgcacct
    79921 tagaaaatgc catgtaccag gccctatccc attttggtac cccggaatgg aactccgaag
    79981 aactggcttt tgcgaaacaa attcaggcta cgcttacctc caacgatcgg caaaacagtc
    80041 tgaataatat cgccgcaacc ggtggcgaaa acggcaaggt ttttgcacta cgtcatcgtg
    80101 aaacggtact ggcgaatgaa gtcgctccat atgccgccac cgataacgtg cttgcggcat
    80161 cgactgatgt cggcgacgtc agttggaaac tgcctgttgc ccagtgtttc agcccctgtt
    80221 ttgccgtcgg tacaccccta catacgtggc aactggttag ccaggggcga acatctattg
    80281 ctcataaagg aatgctgctg gcggcgaaaa ctatggcagc aaccacactc aatctcttca
    80341 ttgattcagg gctattgcaa gaatgccaac aagagcatca gcaagttacg gacacgcaac
    80401 cgtatcactg ccctatcccg aaaaacgtga caccgtcacc tttaaaataa caacaacaac
    80461 gcaaacacaa caaccgagga atgcccatga gtatgtcatc cataccgtcg tcctcccaat
    80521 ccgggaagct ctatggctgg gtcgaaagaa ttggtaacaa ggttccccat ccttttttgc
    80581 tctttatcta tttgattatc gtactcatgg tgacgacggc aattttgtcg gcctttggcg
    80641 tcagtgcgaa aaacccgacc gatggtacgc cggtggtggt gaaaaacctg ctcagtgtgg
    80701 aaggattaca ctggttttta cccaatgtta ttaaaaactt tagcggtttt gctccacttg
    80761 gtgcgatcct ggcgctggtt ttaggtgccg gtctggcgga gcgcgtcggc ttactgccag
    80821 cactaatggt taaaatggca tcgcatgtta atgcccgcta cgccagttat atggtgctgt
    80881 ttattgcttt tttcagccac atttcttccg atgcggcgtt agtgatcatg ccaccgatgg
    80941 gtgcgctgat ttttctggcg gtgggcaggc atccagttgc aggtttactg gctgccattg
    81001 caggcgtagg ttgcggcttt acggctaatt tactgattgt cacaaccgac gtgttgctgt
    81061 cggggatcag cacggaagcg gcagctgcgt tcaatccgca aatgcacgtc agtgtaattg
    81121 ataactggta ttttatggcc agctccgtag tcgtactgac gattgttggc ggcctgataa
    81181 ccgacaaaat catcgagcca cggttaggtc aatggcaggg aaacagcgat gagaaactgc
    81241 agacattgac cgaaagtcag cgttttggtt tacgcatagc aggtgtcgta tcgctacttt
    81301 ttattgctgc gattgcgctg atggtgatcc cggaaaacgg gatattgcgc gatccgatta
    81361 atcacaccgt gatgccatca ccctttatta aaggtatcgt gccactgatc attctttttt
    81421 tctttgttgt ctcgctggct tatggcatcg ctacccgcac aattcgacgt caggcggatt
    81481 taccgcattt aatgattgaa ccgatgaaag agatggcggg atttatcgtg atggtttttc
    81541 ccctcgccca gtttgtcgcc atgtttaact ggagcaacat ggggaaattc atcgccgtgg
    81601 ggctgaccga tatcctggaa agttcagggc ttagcggcat cccggcgttt gtcggtctgg
    81661 cgttgctttc ctctttctta tgcatgttta tcgccagcgg ttccgcaatc tggtcgattc
    81721 tggcccccat tttcgtacca atgtttatgc tacttggctt tcacccggca tttgcgcaaa
    81781 tcctctttcg tattgccgac tcatccgtat tgcctttagc gccagtatct ccttttgttc
    81841 cactgtttct tggattcctg caacgctaca aaccagacgc gaaactgggt acttactatt
    81901 cgttagtctt gccctatccg cttatctttt tggtggtatg gctgctgatg ttgctggcgt
    81961 ggtatcttgt gggcctgccg ataggtccgg gtatttaccc acgtttgtct taagagagaa
    82021 cggatgctga gattacttga agaaaaaatt gccacaccac tgggtccact gtgggtgatt
    82081 tgcgatgagc aatttcgcct gcgggcggtt gaatgggaag agtacagcga acgcatggtg
    82141 cagctgctgg acatccatta tcgcaaagaa ggctatgagc gcatttctgc caccaatcca
    82201 ggcggtttaa gcgacaagct tcgtgaatat tttgccggta atcttagcat tattgatacg
    82261 cttcccactg ctacgggggg gacgccattt cagcgcgaag tctggaaaac actacgcact
    82321 atcccctgcg ggcaggtaat gcattacggc gaactggctg agcaattggg ccgtcctggc
    82381 gcggcgcgtg ccgttggtgc ggcaaacgga tcgaatccca tcagcatcgt cgtaccttgc
    82441 catcgggtta ttggccgaaa cggcaccatg accggatatg caggcggagt tcagcgaaaa
    82501 gagtggttat tgcgccacga aggttatctt ttgctgtaaa cattaaacaa tttgtgccag
    82561 cttgttcaca cttttatgta aagttaccct taacaactta agggttttca aatagataga
    82621 catatattta catctaatat cggaattctc tgctgttaag gtttgcttag acttacttgc
    82681 tccctaaaaa gatgttaaaa ttgacaaata tcaattacgg cttgagcaga cctatgatcc
    82741 cggaaaagcg aattatacgg cgcattcagt ctggcggttg tgctatccat tgccaggatt
    82801 gcagcatcag ccagctttgc atcccgttca cactcaacga acatgagctt gatcagcttg
    82861 ataatatcat tgagcggaag aagcctattc agaaaggcca gacgctgttt aaggctggtg
    82921 atgaacttaa atcgctttat gccatccgct ccggtacgat taaaagttat accatcactg
    82981 agcaaggcga cgagcaaatc actggtttcc atttagcagg cgacctggtg ggatttgacg
    83041 ccatcggcag cggccatcac ccgagcttcg cgcaggcgct ggaaacctcg atggtatgtg
    83101 aaatcccgtt cgaaacgctg gacgatttgt ccggtaaaat gccgaatctg cgtcagcaga
    83161 tgatgcgtct gatgagcggt gaaatcaaag gcgatcagga catgatcctg ctgttgtcaa
    83221 agaaaaatgc cgaggaacgt ctggctgcat tcatctacaa cctgtcccgt cgttttgccc
    83281 aacgcggctt ctcccctcgt gaattccgcc tgacgatgac tcgtggcgat atcggtaact
    83341 atctgggcct gacggtagaa accatcagcc gtctgctggg tcgcttccag aaaagcggta
    83401 tgctggcagt caaaggtaaa tacatcacca tcgaaaataa cgatacgctg gcccagcttg
    83461 ctggtcatac gcgtaacgtt gcctgatttt tccgcataac tcactatcct tctgtcatat
    83521 cattaaattt ttctgattta ttgatctggc agaaggttca tcactgtttc attcaccaga
    83581 tatgggttaa tcttttaatt acaaactgcg ttgacagttg ttgtaaggag accctgtatg
    83641 gctatgtatc agaacatgct cgttgttatc gatcctaacc aggacgacca accagcattg
    83701 cggcgagctg tttatttaca tcaacggatt ggtggcaaaa ttaaagcctt tttgccgatc
    83761 tatgacttct catacgaaat gaccaccctg ctctccccgg acgaacgtac cgctatgcgt
    83821 cagggcgtca tcagccagcg tacagcctgg atccacgagc aggcaaaata ttatctcaat
    83881 gctggcgttc ccattgaaat taaagtggtc tggcataacc gtcctttcga agccatcatt
    83941 caggaagtga tcagcggcgg gcacgatttg gtgctaaaaa tggcgcacca acatgaccgt
    84001 ctggaagcgg tgatttttac gccaacggac tggcacctgt tacgcaaatg cccaagcccg
    84061 gtgtggatgg tgaaagacca gccgtggccg gaaggaggta aggcgctggt ggcggtgaat
    84121 ctcgccagtg aagagccgta tcataatgcg ctcaatgaaa aactggtcaa agagacgatc
    84181 gaactggcag aacaagtcaa ccataccgaa gttcatctgg ttggcgctta tccggtaacg
    84241 ccaattaata tcgcgattga actgccggaa tttgatccga gcgtttataa cgatgccatt
    84301 cgtgggcaac atttgctggc aatgaaagcc ctgcggcaga aattcggcat taatgaaaac
    84361 atgacgcacg tagaaaaagg tctgccagaa gaggtgattc ctgatttggc ggagcactta
    84421 caggcgggga ttgtggttct gggcacggta ggacgcaccg gtatttcagc agcattcctc
    84481 ggcaacacgg cggaacaggt gattgatcat cttcgctgcg atctgttggt cattaaacct
    84541 gaccagtatc agacacccgt tgaactggat gacgaagaag acgattaacg actcctcaat
    84601 aactgacaag gcccgagtta tcgggcctat tttttactta ctgctcttcg ccgccaagga
    84661 aataaattcc caacgggatt gccagcaaca cagtaaagac caggctataa acaaaaatca
    84721 tcgatgactg aatcacatac attgatgtcg tccactccga cagcggaata ttgtactgtt
    84781 ctataacgcc gccgatagtc gcgcgagtca caaccgacgc cgcaataatg aaaaccgcca
    84841 acaaaccaaa gagaaatttc ttccctttcg ctgacttcag tttcgccata atcatgatgt
    84901 aatccttgtg cagacaaatt tcttgttacc taaacgtaac aataccatga catttcacca
    84961 ttgtctgctc tgaaaacctc acctcttagc tggatttaca atcaacgcat tgattagtta
    85021 gctaatgttg ctacttcctt ccctgactat tgatgatctg aatgaattgt cgcggtaaaa
    85081 tagccccgcc aggggtttcc ggcatgacga caattatctg gaggagcctg ataaaacaat
    85141 gatcgctgaa ctgtttacaa ataatgcgct taatctggtc attattttcg gtagctgcgc
    85201 agcattgatt ctgatgagct tttggtttcg ccgcggaaat cgtaaaagaa aaggcttttt
    85261 attccatgcg gtgcaatttt taatctacac cataattatc agtgctgttg gtagcattat
    85321 taattatgtt atagaaaact ataaactcaa atttatcact ccaggcgtta tcgattttat
    85381 ctgtacgtcc ctgattgcgg ttattctgac gattaagcta tttttgctga ttaatcagtt
    85441 tgaaaaacag cagattaaaa agggtcgcga tatcaccagt gcgcggatta tgtcgcgcat
    85501 tatcaaaatc accattattg tggtgcttgt tctgctttat ggcgaacatt tcggcatgag
    85561 cctttctggc ttgctgacct ttggtggtat tggtggtctg gctgtcggta tggctggcaa
    85621 agatattctg agtaatttct tttccgggat catgctctat ttcgaccgtc ctttcagtat
    85681 tggcgactgg atccgttcac cggacagaaa tatcgaaggt acagtagcgg aaattggctg
    85741 gcgaattacc aaaattacga cctttgataa tcgtccattg tacgtaccga actcgctgtt
    85801 ttcatcgatc agcgtagaaa acccaggacg aatgaccaac cgccgcatta ccacgaccat
    85861 tggtttacgt tatgaggatg cggcaaaagt gggcgttatc gtcgaagccg tacgtgagat
    85921 gctgaaaaat cacccggcca tcgaccagcg acaaacctta ctggtttatt tcaaccagtt
    85981 tgctgactcg tcattgaata ttatggttta ttgctttacc aaaaccacgg tatgggctga
    86041 gtggcttgct gcacagcaag acgtttattt gaagattatc gatattgtac agtcacacgg
    86101 cgcggacttt gccttcccca gccagacgct gtatatggat aacattacac cgccggaaca
    86161 gggtcgctaa atcagacatc actggcgtta tccacaaggg taacgccagt tcccatcaca
    86221 tcaatgcttc acaatataca tagtccgact gtacgccaca tcctcagggt tattaatggg
    86281 ataacctttc agccacggtt tgattaatcg tccattggta tattgataaa ttggtgcaat
    86341 cggtgcttgc tccatgagga ttttttctgc cgcgttgtaa tcggcattac gcgctttaac
    86401 ggtattttcc gtcgatgcct gggccagaac tttgtcgtat gccggattgt taaagcgtga
    86461 aatatttcct gaatgcgttg acgttaataa tgtcaggaaa gtggagggtt cattataatc
    86521 ccccacccat gaggcgcgga taacatcaaa attgccggtg ttacggctat cgatataggt
    86581 tttccattcc tgattttgca atttaacatc tacgccaagg ttctttttcc acatcgatgc
    86641 tacagcaatc gcaatttttt gatggttttc tgaagtgtta tacaaaagcg tcagcttcag
    86701 cggtttttgc ggaccataac cagctgcgct caacaaagtt tttgcctgcg cattcagttc
    86761 ttcctgactc atttgttcaa acggcgaagg ttccggcgta aatccggcgg taacatctgg
    86821 tgtaaaatgc cacgctggct tttcgcccgt ccctaatact ttttcggtca tcaggcggcg
    86881 atctatcgtc atacttaatg ccagacgaac gcgctgatct gccgtcggcc ctttttgcgt
    86941 gttaaacgca taataatagg tcccgagctg cggcggcgta taaacctgcc ccggaatatc
    87001 cttcaacagc ttctgataca tatttttcgg gaaggattcg gtgatatcaa tatcccccgc
    87061 gaggtaacgc ttagtggctg cggattcctg attaattggc aggaaggtca ctttttgcag
    87121 taccgttttg gcgttatccc aataatgggt attcggtacc acgaccagtt tttcattgac
    87181 tacgcgctct ttaagaacat aagcgccatt gccgatcaga tttccgggtt tcgtccactc
    87241 tttaccgctt tctacgttgg ctttttgcac cgggaagaag gcaaagttag cggttaaatt
    87301 cacaaaccac ggcaacggtt tatcaagctg aattttcaaa gtatggacat caactgcggt
    87361 gacgccaagc tggtcaggtg tagctttacc atcaataatc gcctgtgcgt tgttgattcc
    87421 cgccagcgcg gcaaaccatg caaatggcga tagcgttttc gggtccacca gacgctgcca
    87481 gctgcagaca aaatcttgcg ccgttaccgg tgtgccatcc gcccattttg cgttatcgcg
    87541 cagggtaaaa gtccagatac ggttgtcatt acttttccac tgagtcgcaa cgccggggac
    87601 aatctcccct ttctcgttct gattcaccag tccttcaaac agatcgcgaa tgacctgaat
    87661 ctctggcagg cccacggctt tagcgggatc cagcgacgca ggctcatctt taatgtggcg
    87721 caccagctcc tgcttctctg ccagtactgt gccgctcgga acttctgcag catacgaaag
    87781 agaaatgctg ctgaccaaca gcgcacaaca cgtgactgaa acagagtgct tcataagttt
    87841 ccctctaacc gacattaaaa ttgatgcgta attatttgtt tcgccttaaa gaaatgcaaa
    87901 caacttccat cagaaagtga cacaggaagc ataaaatgtt ttatgtatca atatattaaa
    87961 tcagatttag tgtaaataat agacgccaaa atgcggcaaa gcacatagaa caccgaacac
    88021 tacgttcggg cttcacacgt tcaaaccagt tttcggtatc ataatcagca tctgtcgcca
    88081 ttgtacgaag aataaaaaat aaccaaaacg ttgaaaggaa ctgagaatac gttaagagaa
    88141 gaattaaatt aatgtatcag caagtagtgc taaacagcac ctttatactt cagcctatct
    88201 ataaataatg agaatgccga gccagcatgt cgacggtgtg gatagtacag gtgataaccg
    88261 ggtaaatctg gtgtgaattt atccaaaaca cgtatcagtt ttttttcttt aatcgcccgc
    88321 tcaacctgat cataaggtag atacgccaat ccatgcccat caattgcagc atcaatgatc
    88381 aggtctatcg tattcagtaa aagctgacct tccatgcgaa cacgaacttc acgtccaccg
    88441 cgtattaatc tccagcgatt tgctgtaccc gatgtgggaa gatacaaatt aattgcctga
    88501 tgatctatta attgtgacac tgacgttgga acacttcggc gagaaaaata atccggtgag
    88561 ccaacaatag ccattggtat atctggcccg attcgaatgg cgatcatatc tttatccatc
    88621 tccccaccca gacggacgcc tgcatcaaaa cgttcagaaa cgacatcagt caaaccataa
    88681 tcaatgataa gctgaatatc aatttcaggg tgtgatttca ggaatgtgcg catggctggt
    88741 aacaatatcg tttttgctgc atgttctaca gtagtaatac gtattgtccc ggatgggcgg
    88801 ttctgcagat cgctcaggga tgccagggca gaatctatgt catgcagcat agggccaaga
    88861 acagacaaaa gatgctcgcc cgcttcagtg ggtacaacac tgcgcgtggt tcgcgtcaga
    88921 agccgcaatc ccaatcgttc ttctatacgg cgcactatct ggcttaaagc tgactgcgcc
    88981 atgctcaggc gggctgctgc gcgagtgaag ctacgctcct ctgcaacgac gacaaacgcc
    89041 atcagatcag cgatctcttc acgcttcatt cagtatctcc atacaatcta ttaatcattt
    89101 aaatgatagg tctaatcatg atttagggtc taatcaactt aatgatcaat atctatagtg
    89161 atatcaccaa ccgttaaaac gaaccaacaa ctccatgagg aacggaatga tgaataataa
    89221 agtcagcttc actaacagca ataatccaac catctctttg tctgcagtga tctattttcc
    89281 tccaaaattt gatgaaaccc gtcaatatcc ggcaattgtg gtatctcacc caggtggggg
    89341 cgttaaagaa caaacggccg ggacctatgc cgaaaaactg gcggaaaagg gatttgtcac
    89401 tattgcttat gacgcatctt atcagggtga aagtggcggc gagccgcgtc agttagaaaa
    89461 cccatacatt cgtactgaag acgtcagtgc cgtgattgat tacctgacaa cgctttctta
    89521 tgtcgacaat acccgaattg gtgcgatggg aatttgtgcc ggtgcaggat atacagccaa
    89581 tgctgcaatt caggatcgcc gcatcaaggc catcggtacc gttagtgcgg tcaatattgg
    89641 ttcgatgttc cgtaatggtt gggaaaacaa tgtgaaatct attgatgccc tgccatacgt
    89701 tgaagcgggt tcaaacgcca gaaccagcga tattagcagc ggagaatacg ccattatgcc
    89761 actggcacca atgaaggagt ccgatgcgcc gaacgaagaa ctgcgtcagg cctgggagta
    89821 ttaccacacc cctcgcgcgc agtatccaac agcaccgggt tacgctactt tgcgcagcct
    89881 taaccagatt attacctatg atgcttacca tatggcggaa gtgtacctga ctcagccgac
    89941 gcagattgtg gcgggcagcc aggcagggag caaatggatg agtgacgacc tgtacgatcg
    90001 ggcatcaagc caggataaac gctatcacat tgttgaaggc gcaaaccata tggatcttta
    90061 tgacggcaaa gcctatgtgg ctgaagcaat ttcagtatta gctccgttct ttgaggaaac
    90121 tctgtaatga aacatattca aatccgcaat agtgatatgg cctggcatat tgctgccaat
    90181 attcagtttc caccaaattt tgatgagtca aaacagtatc cggcgattat cagcgtccat
    90241 ccttttggca gttgtaagga gcagacgtct ggcaatatct atggcaaggc attggctgaa
    90301 aaaggctatg tcgttctcgc ttatgacgcc agtttccagg gagaatctgg tggggagcct
    90361 cgttggatag aagatccgac acagcgcgta gaggatatta gccgtgttat cgattatgca
    90421 gtaactctgc catatgttga tgctgaacgc attggcgtgc tgggtgtgtg tggggggggt
    90481 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
    90541 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attacgatcc gattggcgca
    90601 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
    90661 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
    90721 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
    90781 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
    90841 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
    90901 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
    90961 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
    91021 gaaaaagtta ttcctttttt caacacccat ctgtgataag tttcatgaga aatattatga
    91081 aaaatgtact cattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
    91141 cagataaaca aaccattaaa caaactttat ttgctcgtca gccagcaaaa atacacaaac
    91201 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
    91261 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
    91321 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
    91381 tttccctcgg catttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
    91441 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
    91501 aatatacaat tcttcgccct gcctggctga cggatgaaga tattattgat tatgagctta
    91561 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacc taagagtgtc gcagcattaa
    91621 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
    91681 ccgggactga tggcgacaag ccattcttca tgtaaatcaa ttatataacc accatcgtac
    91741 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
    91801 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
    91861 taatatctgc acatcaatga aaatacaaac aacttccggc aaaaaagtga tacaagatcg
    91921 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
    91981 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
    92041 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
    92101 tccggcccct gcagccagtc gcgaaagtgg tttgattctg gctggcactc acggtgatga
    92161 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
    92221 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
    92281 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
    92341 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
    92401 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
    92461 ggtggtctca ttccacgatc cactggcctg tattgaagat cccagacaca gcgaattagg
    92521 cgaatggctg gctcaggcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
    92581 tggctctttc ggcagttggt gtgcagatct gaacttgcat tgcatcaccg cagagtttcc
    92641 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
    92701 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
    92761 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
    92821 gcgctaatgg cccgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
    92881 cgcgcttcag tcgccagcgc cagcgcttcc gtcagacccc cggttttatc gagcttaata
    92941 ttaaccatct catagcgccc tttcagcgcc ttcaaattgc tacgagtatg acaactttca
    93001 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
    93061 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg tgccgccaac
    93121 ccttctgcat gccaggattc atttgcatca acgatcagcg tggtatcggg cacagctgtg
    93181 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
    93241 gcgccggcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgact
    93301 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
    93361 tgctgctgtt ttcgcgcggc cagatcccac aatgcacaat ccagcgcatt acgtgctgcg
    93421 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
    93481 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
    93541 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
    93601 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
    93661 ttaacggttc tcatctttac tccttggtgt cgcggtggtc tgcggattaa gcgcaaatag
    93721 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
    93781 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
    93841 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
    93901 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
    93961 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac cgagatcgac
    94021 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
    94081 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
    94141 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
    94201 gttattgatg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
    94261 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
    94321 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctggcttaag ccatattccc
    94381 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
    94441 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
    94501 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
    94561 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
    94621 cgttctttaa ctgccgcaca tttcccggcc acgcataacg cgtaagtaca gtattcaggt
    94681 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
    94741 cagttaacgg catgatgtcc tgcggacagt cacgtagcgg cggcaggttg agcgtcaaca
    94801 cgttcagacg ataatagaga tcttcacgga acatgccttt ttgcaccagt tcgaccagat
    94861 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
    94921 gacggaaagt gccatcatta aggaaacgca gtaatttcgc ctgcatccgt ggtgacatct
    94981 cacctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaat cctttcttcc
    95041 cttccggagc atgaccaaac agctcactct cgaccgcatc ttccggtata gacgcacagt
    95101 tcagcgccag gtaaggtttg ctcgctcttg ggcttgcctg atggcaggcg taggcaaaaa
    95161 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
    95221 tctgcgcctg ttcgacaaca tgcttcattt tcgggctgac agcgacaatt tgactgaagg
    95281 cgctgacgtc ctgggcggcg acattttgca actgacggcc catacgaatc gttgatcgca
    95341 acatcaccac cgcacctgtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
    95401 taatctccat caggaagttc tgtccattaa taacaacatg ctcgttatgg gaatcttgcg
    95461 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
    95521 tgcgcagtcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
    95581 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
    95641 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
    95701 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
    95761 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
    95821 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
    95881 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttgaat
    95941 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
    96001 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
    96061 gcagcgtttc tttcacctga ccgataagct gacgacgaaa atccccgagg cgaggtttgt
    96121 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccc agtcgtgccg
    96181 tcagaagtcc tgcaccaatc ccctgagctg cgcgggtaga caaacgagca gcgagatctt
    96241 gcgacatcca gtccattccc acttcgcgca ccaattcgct ggctccggcg aaagcgatat
    96301 tcagcaatac cagcttgaac aggcgcaaac ggctgtaata tcccagttca atgccataca
    96361 gcgtggcgat gcgattaatc aaacgcagat tgcgccaggc gataaatgcc atatccacca
    96421 gcgccagcgg gctgaccgca atcatcaacg ttgattcagc tgctgagcgg ctgatttcgc
    96481 gccgcgcctg ggcatctaaa accggctgga ccagatgagc atacaggctg accacttcac
    96541 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgctgga tgagactgat
    96601 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg gcctttgccc gtgccgtggc
    96661 tgtgcaacaa atcgcgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
    96721 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcagcac
    96781 atccacccag cgccacccag tcctgagttt gccaggcatt cattgtccac tgtacaccct
    96841 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
    96901 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
    96961 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
    97021 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
    97081 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
    97141 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
    97201 ttgcttatcc cagaacgcct gaccaggcaa acgtgcggga acttcgccag gataaacagt
    97261 gagcggtgcg ccatcgctaa gtcgattacc gcgcagcgcc gggattttct caccgttaac
    97321 atcaataatg ccactggtgg tcgcctgaac tgacgccagc cccaggcaat ccatgctgat
    97381 cccttcgaac gccgcatttt gccaggcatc ctgaattagt tgttgcagca atgaaaccat
    97441 attggcgtgc tgatcgatgg tcacatggtc cgctttagtg gcagcaaaca atagcttatc
    97501 gataaccggc gaaaacaaac gcctgaacag ggtacgctgc ccatagtgga aactttgcat
    97561 cagctgcgtc agcgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
    97621 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
    97681 cttcaccacc ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
    97741 cttatcggcc tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
    97801 ttgcagcgcg ggcgcacctg ccatatcccc cggcaagaca aagcgcccag gctgaataaa
    97861 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacgcggcgg caatgtctgc
    97921 cagccggttt tcgtcggcag gcgctagcgg gtccagccct tcgcacatca ttcgccattt
    97981 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
    98041 atagtcctgc gccagcatcg gcaggtcgag caaccattcg ccagggtaat ccacaatctc
    98101 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttatagcg
    98161 tagtgccagg cgaatttcgc tgacgccgcg cgttggcgtc ggccaggcgg gaggatcgcc
    98221 atacagctgc gccaaccctt cgtcgtaggt aaaacgcgga atgccaaagt cacgctgagg
    98281 aatgcgtttt acgcccagca gacgctcttc acgcaccgca cttaatagcg gcaaacgtgc
    98341 tccggcatga atattgagca actgattgac catcgcagtg ataaacgccg ttttgccgct
    98401 gcggctaagt ccggttacgg cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
    98461 attaagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
    98521 aatcattcat aaggatatga atgtgggggc caggccattg attatcaact acccgtgttg
    98581 tcatcgacgg gcgaactcca taagatgtgc agcattcacg ttcggacgga gttcctgacc
    98641 tatgtcccct actatttatg atattgccag ggttgcaggc gtatcaaaat ccaccgtatc
    98701 acgcgtgctg aataagcaaa ccaatatctc cccggaagcg cgcgaaaaag tgttacgggc
    98761 cattgaagaa ttacagtatc aaccaaacaa gctggcccgc gcgctgacct cttcgggttt
    98821 tgatgccatt atggtgattt ctacccgttc gaccaaaact acggcgggta atccgttttt
    98881 ctctgaagtt ttgcatgcca tcaccgccaa agctgaagaa gaaggtttcg acgtgatatt
    98941 gcagacgtcg cacaccccgg cagaagactt acaaaaatgt gaaagcaaaa ttaagcagaa
    99001 aatgattaaa ggcattatta tgctaagttc gccagcggat gagtcatttt ttgcccaact
    99061 tgataaatac gatattcctg tggtggtgat tggcaaagtt gaaggtcaat atgcccatgt
    99121 ttattctgtc gataccgata attttggtga cagcattgcg ttgaccgatg cgttaattga
    99181 aagtggacat caaaatattg cctgtctgca tgcaccgctt gatgtccatg tttcagtgga
    99241 tcgggtaaat ggttataagc agagtctgga agatcataat attgcagtgc gtgatgaatg
    99301 gattgttgat ggcggttata cccatgaaac agcactgcaa gcagcacggc aattattaag
    99361 tcagtcaccg ttgcctgagg cagtgtttgc cactgacagc ctgaaattaa tgagcattta
    99421 tcgtgcggcg gcagagaaaa acattgctat tccgcagcag ttagcggtgg tgggttatag
    99481 caatgaaacg ctgtcattta ttttaacgcc tgcaccgggc ggcatcgatg ttccgacgca
    99541 ggagttaggt cgacaaagct gtgagttatt attccgctta attgccggaa aaccgtcacc
    99601 acaaaatatt accgttgcca cgcatatgac gctgaaataa ataccagcga ccttgcctga
    99661 atgccggatg cgatgctggc gcatcttatc cggcctacat ttatagatcg gataaagtac
    99721 tcgcgccgca tccggcaatt tagcccatta tcagaacgag taatttacgc cgacacctgc
    99781 ataatggaat ttatcgctgt cgccttcgtc gtgatcctgc cactcaaacg cgtattccag
    99841 cgaaatggaa agaccattct ggaaatcata accgtaaaat aaaccaacac ggttaaaatc
    99901 atggccttca cgttcaatat catcctgcca gtcccagtta ctccagcgat ccagcccaat
    99961 gcgcgtatac ggcgtcaccg agtggttgcc gagcgtcagc ggcaaatagg cgcgaatttc
   100021 ttgcgtggaa aactcaccgt tattacggct gtcgtccata ttgaagccgc gctcgagata
   100081 atagttcact cgcaaggcaa ccgtttcgtt gaaggtatat tgcagacctg tttccgtttc
   100141 gacacgggta tcagcgtaac cggtcgtgtt caaatcgttg gcaaatttat acatcgacaa
   100201 ccaaccgttg aaacgtaaat cgtcagtcag tttcacatcc cagtctggcg cgattttcca
   100261 gcgctgcata ttcgccgtgt ctttacccgg ttcatcaacg tagtgataac cataattacg
   100321 gaaaccgccg gtcaggccga aactgaaatc atcgctttcg aggaactgat aatgtacctc
   100381 cagctccggg cgatcaaacc acgttccacg tttacccgcg ctataatcta ccggcccttc
   100441 ctgataatag gccagagaaa ttctccacgg cccgttggcg gcattaaaat agacagaagg
   100501 ctccgccagc ccatccatat cttcgccata accctcgacg ttttctattt cgtacatcgc
   100561 gccgatatta aagtgccagt cgttcttttc ctcggcctgt gcgcaggcca ttcccgcaca
   100621 catcaccagt gcggtacagg gtaataactt tttcattatt gttccttgta ttatcctggg
   100681 ggaatgcctt ccccctgtat ttagcgaatt gctatttccg tttctgcatc aaagaaatga
   100741 catttagtca tatcaaaatg aatagtgata ttttctcctg catgataatc atttaacgca
   100801 ccagcacgga ccactaactc gtgcccccca accgtggtgt agagcataaa ttccgcaccg
   100861 gttaattcta ccacgctaat tttggcggaa atattatttt cctcttgcgc gtccggatga
   100921 atatcttccg ggcgcattcc catcacgatg ggcttatgca aactttcctg tgttttcaga
   100981 accgctaatt tctcttcggg aatggttaat ttaagcgttt ccgtaacgaa tttatcgcca
   101041 tcgatcgtgc cgcgaataaa attcatcgct ggtgatccaa taaatccggc aacaaacata
   101101 ttcgcgggtt ggttataaac ggttttcggc gcacccactt gctgaacaat cccgtctttc
   101161 ataatcacaa tccgcgtcgc catggtcatc gcttcggtct gatcgtgggt cacgtagatc
   101221 atggtggtgt tcagtttctg atgcagcttg ctgatctctg cgcgcatttg tacgcgcagc
   101281 ttggcatcaa ggttagagag cggttcatcc attaaaaaca cgcccgcttc gcgcacaatc
   101341 gcccgcccga gcgccacacg ctggcgttgc ccgccggaaa gcgcgcccgg cttgcgcttc
   101401 aggtactcac gcaggccgag aatttgcgcc gcccagttca cccgctcatc aatcacctct
   101461 ttggcgattt tttgcatctt caggccaaac gccatgttgt cgtaaaccgt catatgcgga
   101521 tacaacgcgt agttctggaa caccattgcg atattgcgtg ctttggctgg aacgtcattc
   101581 attcgtttgc cgtcgatcaa cagatcgccg ccgctgatct cctcaagccc ggcaatcatg
   101641 cgcagggtgg tcgatttacc gcagcccgac gggccgacaa atacgatgaa ctctttatcg
   101701 gcaatttcca ggttgaagtc cttcaccaca tgcacctggt tatcgtagat tttttgaata
   101761 tgttgtaacg aaagctgagc catgttgatt cctttgctat acgttttgcc agaaggccga
   101821 taaccgcggc caggtcaggg agtccgttga gggcaacaat aattgcgccc cggttaagcc
   101881 tgcgccgatc cccaccgagc gcataccgct ggcgttaatg gcgtcaatgc ccgcctgcgc
   101941 atcttcaatg ccgatacatg cctgcggcgg cacgcccagc ccggcacagg cagcgagaaa
   102001 gatttccgga tccggtttcg agtttttaag ttgggaagca tccgcgcaga aggtgaaaaa
   102061 ctcgcgcagc tccagcgccg ctaaaatcgt cggcgcattc agggagacag aagccagccc
   102121 taccgggatc tgctgtgcgc ggagctctgc cagcaaattg cgaataccgg gcagcacagc
   102181 attaaccgtc agctcgcgca gtgagtggac gtaaagcaga tttttgcgat acgccagttg
   102241 cgtccgctcc tgcgggttaa agtcgccctc tttgcccccg tgttgcagaa tgcgccgcag
   102301 agactcatcg cggctgatcc cttttaggga ttcgttaaac tgcgcatcaa tgctgatgcc
   102361 aatttcagcg gcaatctgct gccacgcctg gaaatgcaga tgcgcggtat cggtgattac
   102421 accatccaga tcgaaaatta ccccttgcag tttcattcat cctcctgatg cgtggtagcg
   102481 gtcccattga agggcaaaat aaaatcaccc aaacagaaaa cagattcttc agccacggat
   102541 ataagctgcc cgttcaaacg cagtgaaacg ggcgcagaag ttcgaatcgc aatacgctgc
   102601 gcgtcgagcg tgacctgtaa ttcgcagccc tgccagaaca gagggaaaga caactgttgc
   102661 cactgctccg gtaacgccgg attgagatgt aattcaccgt cacgcacgct caccccggca
   102721 aaaccctgaa tcgcccccag ccagatagcg ccagttgcgg cagcgtggat accatcatca
   102781 caactatgcg gatcagcacc aagatcgatt tcagtcccct cgcgccagaa ctgataactt
   102841 tgggtcagca ggccacagcg tgcggcaaca atgccgtgga ttgctttact taatgacgag
   102901 tcgtgaatag tgcgcggttc ataaaattgc agattggcaa gacacgatac cgctgagaac
   102961 tgctccggca gcatgtaatt gagcatcacc acatcagctt gtttgaggat ctgcatctcg
   103021 ttcacttctg cgcgtgaata atccagcaga atggtttgct tccccgccgc cgctttgtat
   103081 ttagccagat taatcgccgg cttagccata aacgaatcat cctgcggcaa aacgccatcg
   103141 ggctgcgttt ctggcatcca tagctctttg aggaacattt cggcgcgatg gataaacgca
   103201 tcgtcgctac agccgaactg gcgggcaata ttcagcgcct gttgaacgtt gtagcgggcc
   103261 atatagctgg tgaaggcgtt attattgaca tgttcggtat attcgtctgg cccaataaca
   103321 tcatgaattt ccagacggtc gttaaccctc accgcgcggc taatccagaa ctttgcagtt
   103381 tccagaagta gcgccatgcc ttcatgagca atgaaacttt catcccccgt ggtctgccag
   103441 tattgaataa ccgcccaggc gatatcggcc accagatgat gttccgcctg cgccgaggcc
   103501 actttttgcc gcagcccggt acgaatgtta atggcggcaa attctggcgt ctcttcttcg
   103561 ccgctgcgcg cgctttccca cggaaatagc gcgccctgcc agccgctgcg ccgtgctttc
   103621 tcctgcgcgc ctggcaagtt gtgccagcga taacgcagta aacttcgggc aaccgtcgga
   103681 tcgctaaaca gatggaacgg cagcaaaaat acttctgtat cccagaaaac gtggcctttg
   103741 tagccttcgc cggttaagcc ttttgccgca atactgctgc gctcgtcgtg agccggcgtc
   103801 atgatgcgca gatgataaag cgcataatct aacgcttgct gatcgtgcgc atcgccgcca
   103861 tttaccgtga tacgacgttt ctgccaccat tgacgccagt tttctgttga tgctgcaaga
   103921 agttggtcgt aactctgctg cgcgcatatt tcaagctggc gaagcgacgc gctgccccac
   103981 tcgtctaaaa cggcttgcct gtcatcccgc cagtcgatcc acaccagttt ttgcaacgtc
   104041 actgtctcgc ctgcatgaag ctgcgcactg gtatgttgca gcaaacggcg ctctttggcg
   104101 gtatagcatt gctgcacatc accgctcacc ttacagcaac agctgatggc cacatcactg
   104161 cgtccatcct gggtggtgta gatcccctgc atcagatgct gaccaaacac ccgcacctgg
   104221 gtttcgtcga gatgttggcg accgtggttg gtttgcgtgg cgtcgatacc tgttgaaatc
   104281 agcactgacg cgtcggcgtc cagtggcgta atagtgattt ccagcgcaat gagcggcagt
   104341 tggtctgccg aaacaaagcg acggctggcg atggtgtaac ctgcgccgtt gctggtacgc
   104401 cagacaacgt tgcggcgtaa ttcgccactg gcaaagtcaa gctcacgctg ccaggcttcg
   104461 tgggataacg agaaaacctc accatttatg gcaatctcca tccccaagat atcaggcagg
   104521 ttcaccagtt cgttgatttc accttttccc gcccgatgat acagccccgc cagatacatc
   104581 cctcgcgtct ggcgcgtgta atcttcttca tggctggcgc gaagcccaag atagccgttc
   104641 ccctgcgcca tcagcgatgc atacttgttc agggtatgct ggctgaaatg gggttctgat
   104701 aacgttactg gcctggtcat aattccacac gcgtccctgt ttctgcggat tgatataacg
   104761 ccgccaccag ttgctggatg atgtacccct gctcggcgtc ggctatcatc acgggcttgc
   104821 cctgtacgtg attgataaag gcttccatgc tgcccaaatg gcggttgtcg tctgccattt
   104881 cccgttgcat cagcgtcatt aattcaccgt tgttatcggt gtagatatgt gctggaaaca
   104941 gcgtcgcacc ggctttatca ccacaaaagc tgacgttcat aatcgactgt tcgcggatgt
   105001 tgagcgcaaa tgacgtttcc agccacagaa tgccgccgtt atgaaattca atggtgccaa
   105061 acagcgaatc ttcgacgctg taagttgccg gatcccactc gccaaattga ccacagctct
   105121 tttgcgtgcc gatcttttga aagctatgcg cattcacgct tttcaccgca ggatagccca
   105181 gcacatacat cgcagcatcc agcatatgaa tgccgatgtc gatcagcggg ccaccaccct
   105241 gcagttcttt attggtaaag acaccccagc cgggaacgcc gcagcgacgc agggcgcggg
   105301 cggtggtaac gtaaatctct cccaaaacgc cgttggtcac ctgttcacgc agctgttgcg
   105361 tatcgagcgc aaaacgatgg tgaaagtcgt aggccagcac cttgccctgt ttgcgcgcgg
   105421 tatcgcacat ttcccgcgcc tgttctggcg tcatggcggg cggtttttcg cacatcacat
   105481 ggcagcccgc ttccagcgcc atcagggtat gttcgtaatg aaaacggtta ggtgagcaga
   105541 cgctaaccac atcaggtttc accgccagca gcatggcctg cggatcgtcc cacacggatg
   105601 cattcccgta tttttctgcc agcgcctgcg cctgggaaag gcggctgtca cagacagcca
   105661 ccagttccag atcgttgcgg gtgcagtagt acgaagcatg aaccttatcc gccacctggc
   105721 ctgcgcctat tatcgcgacc cgcagtggag agcttgtcat tgcacttttc acaaagacct
   105781 cttagcaggt acgcaaccag gtcaacgaat cacggtacgc ctgggcagga tcttctgccc
   105841 ggatacgccc ttcatacacc acataaccct gatagttatc ggcgcgcagc tgttcaaaca
   105901 gcgcgtggaa atcgagggtg ccgctgcccg gctggtaacg atggttatcg gcaatatgca
   105961 catgaccgag caagtcgcgg ttgtcatgca gcgcctgcgc caggttatct tcttcgatgt
   106021 tcatgtgata gaaatcgccg ataatctgca catgtttaag attgttttcg acgctgtaac
   106081 gacgagcatc ggcgagggtg ttgatcatat gatcctgata gcggtttaac ggttcgagat
   106141 acaccacggt tccggtacgc gcggcgacct gttccagtac gcgcagggaa tcactcacca
   106201 ttttgcggtc gccgtccagg ctacgcggcg aggtcatcgg cggtaagcgg aaggtaaaca
   106261 tgccccacgc tgccggaacg acgatacctt taccccccac ttctgccagc gcttcgagaa
   106321 tgcgttcgat ctgctttaag ccattaagac gacgctcttc gataaagtca ccaatccagc
   106381 cgtcatagcc gccgcaggcg gtggtcaccg gtaaaccggt ttctttgatc gccgatttga
   106441 cttcttcgat gttgttgacc agcagtttgc cgtcaatctc aaaaccatcg aagcccatct
   106501 ctttgatata acgaaatttc tccagaatat tttccgggaa gaacgcctga ttctgtgtgc
   106561 ctattttcat tttttcattc ctttgaatta aaacgtgacg cccattttga tgctctgttc
   106621 cggatgctga tcgacatact gcatgtagct ttccgggctg ttggcaaagg tcactaccgg
   106681 gtcgattaaa tcttcgcaat tgagataacc gttcatcagc agtttccagc aggtttcttc
   106741 aatacgctta cggctccagc gcggataatc cgggttcggt tcactgcacg cgcgagagaa
   106801 gacaattttg gcgttattga aatgcgcttc gcgtccgagg ttaaaacctt cagcaaacgg
   106861 tttagcaaac gcgacatagg agatggtgcc accgtaagcc aggccgcgca gcgccgactg
   106921 cagcgcgtcg gcgtaaccgc tggtttcgat aatcacatcg gcaccctgct tgccggtcag
   106981 cgttttgatc tctttgccta cgtcagtgcc aatgggatta aggcagaaat ccgcaccgtg
   107041 acgacgagca atatcacaac gatgggcaat aggatcgacg ccaattacca ccgacgcgcc
   107101 agcgcgttta gccagttgga tggcaatttg accgatcgcg ccaagcccta ccaccactac
   107161 aaaatcccct acgcgtacgt tggcatcgcg aacgccactc atggcaaact gcgccgggtc
   107221 gtagcagaca gcgtttttcc aggagctgcc ttccggcatt ttgcgcagct tgtagttatt
   107281 cactgcgtta atgatgatcg tctcggagag cgggccgtag ccgcatacgc tgtcgcccac
   107341 cgcgtagtcg gtaacgtcgc tgccgcactc gataatgtcg ccaaccacca tgttgccaag
   107401 ctggaatttg ccaaactcaa tgccgcgcgg cgcatctgcc ggacgcgggg tgaacatctg
   107461 ccattcgccg ttaaagtctt catcgataaa cgggctggcg gcgcggaagt cgaccacttc
   107521 cgttccgtgt ttcggtgcac cgaaacgcac gcggatcttc acttcattgg ctaaaatcgc
   107581 ccgatcttca tactcaacca gcgcagcaac gcgcggtgct gtggctacta actttttcat
   107641 gtaaaactcc ttgtaaagaa actggccgct ttagcccttc acgccaccgg cggtcaaacc
   107701 actcttaata aaacgttccg aaagcgcata cataatcacc accggcaatg cggtcaccag
   107761 cgaggccgcc atcatccgtc cccagatgta atctggcgta ctgaacagcg cgttcaggcc
   107821 gactggtaag gtgaaattgc tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt
   107881 ccatgccacc ataaagcagt agacgaatac ggatatcagc ccggacatcg ccaggggcac
   107941 ggtgatacgg aagataattt gcagacggtt gaggccatcc atcatcgccg cttcttcgat
   108001 ctcatcaggg atggtgtcga agtaactttt cagcataaac acggcggtgg gcagagtttg
   108061 cgtcaccatg gtaatgatca gcgccatctc ggtgtcgtaa atgccaagtg cggtgataat
   108121 tttgaacagc ggcaccacca gcaaaatgcc ggagaacata tacaccgtgt aaaagctggc
   108181 gttgatggtc atccggcctt taaagcgcag gcgagaaagc gcgtaagctc cgagaatgcc
   108241 gagaaatacc gccaccacgg atgaaaccac cgacaccacc atgctgttac ggaagtagtc
   108301 gacaaacggg aaaatcaccg ggttaaaaat gtcgacataa tgctccagcg tccactgttg
   108361 cggcagcagc gtaggatgca gcgagatcgc ctcttttgcg ctcttgaacg aggtcatcag
   108421 catcacaaaa aacggaaaca gcgtgatgat gagaaacagc gccagcccgc ggtaaaaacc
   108481 gatacgactg agtgtgcgtt tatttgttgc cattgaggtt cacccgtttc ctggtcagca
   108541 gaatgacagc gaaaataatg atgaagagca ccaccgagat cgccgccgct ttccctaagt
   108601 cattaaaggc aaaggcggtt ttgtagagat agacaccaag aatatcgacc ttggttgtca
   108661 gcagataaac atccgcgaac atgtagaaca tccagatggt gcgaagggtc accaccgtcg
   108721 ccaggaccgg cataatcgcg ggcagcgtga cgatacgaaa ccgttgccag gcattagcgc
   108781 catccatctc tgccgcttcg tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg
   108841 agataaaggc atacgggaag tagcgccaga tggcgaagat caccaccagc acaaaactac
   108901 tgcccggatt atcgaaccac agcggtgcct gctcataaag atgcagaaga tcgacgccga
   108961 ggtagttcac aatgccgtag ccgttgttga acatgtattt ccaggcgaac accagcgaaa
   109021 tagacggcgt tacgtaggag aggatcacca gcgaacgcgc ggttttgcgc aggcggaact
   109081 cgcggttgaa aaacatcgcc actgccagcc cgagcacggt gctgcccgcc accaccaacg
   109141 cggtatacca gaccgtcatc cacagcgagt gccagaagcc gggatcggag agaatacgca
   109201 catagttgct taccccaaca aacgttgact cgatgttggg attgagcggc agacgtaaaa
   109261 aactaatttc gatattcgac accattggcc acgcaaccag accgcccagc agtaataagc
   109321 tgggtgcgag aagcaacagc gcaaagggca tatcggaacg acctgaaaaa agcctgttca
   109381 ttgcggattc ctgtcctggg tttagtgctg ttcgatcagt tcatccagct ttttctggct
   109441 cgtttgcagc gtgccaggga gatcggcttt acctacggtg acgttatgca ccatggagct
   109501 caccacccca gaccccgtca catcgcccat gcgggtaaat tttttatccc ctactgcgcc
   109561 aaagacctga atattcggca gttcaccgat taactgattt ggcagttcac ccagcgcctt
   109621 aataacgtcg ttgtctttcc aggtggaagt agtgacaacc gctttgttca ccggcagcgc
   109681 cgcgcctggc gacatcatca cccagtcggc aatgttgtct gcctgctcca taaaggtgac
   109741 aaatttttca gccgcttccg tttcttcagt cttttgtccg gcggtaatgg tcagcgaggt
   109801 caacatgccg tagacggcag agtttttctc ggttggcacc acgaaaccga cgtttttcgg
   109861 atcgccttct ttaatcacag ccggaaggat ataggtggag taaatcgcca tcggcgcggt
   109921 gccgttcata aaggcgtctt tcacttccat gatgtcgtta gaacccggca tggtgttggc
   109981 agcaaggtcg cggtaatagg tcagcgcctg catcatctct ggtgaatcaa gggtgatttt
   110041 gccttcggcg ttaaagacgt tggcctagtt ggataacgca aactgggaga aggattgttc
   110101 cgtcagcacg ctttctgccg taggcagcgc aataccgtat tttttattcg ccgggtcatt
   110161 cagtttctgt gcaacgtcca gcagctgttg ccagtttttc ggctcctcca gccccgcttt
   110221 tgccagcaca tctttgcgat accagatgcc gccaatccag gcgctgacag gaacaccggt
   110281 ccatgcgcta ccatcttcgg tacgcacaat acgcagtacg ccatcgtaaa acgcgccttc
   110341 accaacgttg ctgatgaccg tggcaaccgc cttgcgatcg ataagctgct ctttgtccat
   110401 aactttggcg tagtcatgac tggtttcaat cacttccggc agcgagccgc tacgtgaaag
   110461 agtgatgact ttggtgttat aagcgtcctc ttcgacgggc acttgtttaa cggtgatacc
   110521 tgggttttct ttttcaaaac gggcaatcaa tttgctgata actgcctgac gctcctgctc
   110581 caccgaagaa tgcataaatt cgatggatac atggggttta ttttcttctt tacagcctga
   110641 aatcagggcg caggaaacca gtgctgataa cagcacgatt tttgatttaa tcatgagatg
   110701 ttccttttta tttaatccac ataacctgcc aggggcgcag cgttatttta ttacccaata
   110761 tatcaacttc actaatcaga tcccgaccgt gcaggccagt aatatttaca tgctgaatat
   110821 ttttactgac attaaacaat ccggtcagac aattaccctc tgcgttactt ctttgaatac
   110881 gcattacgga tgaattaacc gtatcgatgg taaaattatt atccggatga aactcgttat
   110941 ggctgcgacg aagtgtaatt aaacgcgaca actcatgata taccgcatgc cttaatgtag
   111001 cttcatcgtt cagttctcgg gttatctctt cactgtgata ttttttacgg ttaatcgcac
   111061 ggttatatcc gagtttttcg acacctgcgt aatcattacg cgagcccaga atactttgaa
   111121 tatatatcgc tggcacaccg gggaaactta acaaaatcgc atgggcaagg ataaacctgg
   111181 cgcaacgttc ttcatcgcta ctctcgcggc ggcttaacgc atccatatag gtgacattta
   111241 tttcataggg actgcgcgta ccgtcgggat tatttttcca gtttactaat gcaccttcct
   111301 gctgtaatgc ctcgaccagc gctaatattt cgctttctgg taacaagccc cgcagtgggt
   111361 tcagcccgat gccatcgtga gaggcgagga agttaaacca ggtcgtttta ccggagggca
   111421 agctcagact ttgcgcccac tggcacagcg cctcgacgtt ctgtttttgc accgcatgta
   111481 acaccagcgg cggcagcgag aactggtaca ccatatgcgc ttcgtcatcg ccttcgccaa
   111541 agtaagcaat gttatcttta tgcggaacat tcgtctcagt aataatcacc gtacctggcg
   111601 cgatgtcatc aataatcgac cgtaacagtt tgataatcag atgtgttttt tccagatgga
   111661 tgcagcttgt tcccggctct ttccacataa agccaacggc atccagacgg acatattctg
   111721 cgcccttttc caggtaacaa agcaaaacat ccaccatcgc cagcaacacg tcagggctac
   111781 ggtaattcag gtcaatttga tcgtcgctaa aggtggtcca taaatggcgc gtggaattat
   111841 catccatttg aaacggcgtt aatagcggta atgcacgcgg acgggtgaca gcgctgagat
   111901 cggtttgcgg atcaaccgca ataaaaaaat cttcaaaacc tggctgctgt tgtaaatagt
   111961 ttttaaacca ttcacttttt gccgacatat ggttgcagac aaaatcaaac attaaatgac
   112021 tgcattcacc gagctgctga atatcctgcc actacccgct tcactggcga cctgatgata
   112081 atcaattacc gaaaatccat catcagatga ccatggataa aaaggtaata aatgaacatg
   112141 tgaaaaaatg ctttgcagcc attgatggta aaactgatta aatgtgggta gtggttttaa
   112201 atcattactg tgaaattgat cggcataggt aattaacacg acatcacttt catcccagtg
   112261 ttttttacgt cgctgtgtaa ttaatcgttt cgcactctca agacgcgtta caagtttctg
   112321 taaatgagtt gcggtaaatg ttccaccgta gatttcgtcc aggtaatccg taattttctg
   112381 tttcatgttg cctttcctgg tagcggtccc atgaaatgag agactactcc gcagaaaata
   112441 aactgtcaat tgagcacaag ggttttagtc acgcaggaaa tcatttttta ccagggaaaa
   112501 agcgtatttg tgagctgacg caaataatct atagggatgt aaataaggtg tgacagaaaa
   112561 aaacggcgca taagcgccgc tcatggtgaa ttcttttaac ctttgacctt cggcattgcg
   112621 atgtctttca ggccaccggc gttctcaacg tgggtatatc ccatctcgct aaggatctct
   112681 tttgcctgcc ctgattggcg tccggcattg caatacactt tcacggtgtc gtttttatcc
   112741 ggaacgacgg tggcaatgcg ctctttcact tccttcaggg gaatattgat ggccccctga
   112801 acgtgctctt gctgatactg ctctggaaca cgaacatcga tccagtgttc agcggcgaaa
   112861 acgggcagtg aaaacaccag tgccagagca agtaagcctt ttttaaacat gtaagcgtcc
   112921 ttctatggat gaatattgct atccattata cgcctgagat ttaacaattt ttgcgaaact
   112981 cccctcacct tttataacgc tgcgccagtt tattggcagc ccgacttaac agcggttcca
   113041 gcgccacagc cagcaacatt ttcagcggtc ggcgagcaac tgattttatc gcccagcccg
   113101 ccacacccgc tgggccatag cgcagtgcgg taagaagtac cagcttgcct gctaatttga
   113161 aaccaggctt taccttttgc ccggcctgtt gccagcgagt attcataact ttcctcacag
   113221 ttgacggaaa cggctacgta acgtgaatgt atcggaagtg acataacgtt ccatctcgcg
   113281 taaacgcgtt tcacttgccg ccagttcacg gtcgacttca tccagcaatt cactgctgga
   113341 aggtagctgc tcaccaaagg ccatgttgtc cggcattgga tcaagcgcaa atgacaaaat
   113401 gatgtaagca accagggtaa acagcgccag accgaagaaa atcgacaaca ccaccaggat
   113461 acgcaccagt tttaccggta catcaaaata gttggcaatc ccggcgcaga cgccacggac
   113521 catgccctgc tgtggaatac gccataattt tttattgaga ttaatgcccg ccattagcga
   113581 tccctccagt tcggatgttc tgcatcaaga atagattcca gcgcctgaat acgttcgcgc
   113641 atccgttttg cttcatcagc cagttgcgct aatcgctgct gctcactttg cgacaattca
   113701 ctgcgaccag aacgattgct gtaatgcagc cataaccaga tcggtaaaac aaacagcaca
   113761 aaaatggtta atggaatagc cagaaatagc gcgctcataa gtactcctta gacgatgagc
   113821 ggcggcgcgt tcggacgccg cctggatgtt attattgatt gtcttgcttc attttggctt
   113881 ttaattgtgc cagttgctcg ctgattgcat catcggcttt cagttcggca aactgatcgt
   113941 ccagcgattt ttgtttaccg aagctgtggc tttctgcttc cgcttccatc tggtcaatac
   114001 gacgttcgaa agattcgaaa cgagccattg cttcatccag tttgccactg tccagctgac
   114061 gacgcacatc gcgcgacgag tttgccgcct gatggcgtaa catcaatgcc tgctggcgag
   114121 cgcgtgtttc gctcaatttg ttttccagct cgccaatctc tttcttcatg cgtgccagcg
   114181 tatcgtccac cagcgtcact tcatgttcca gggacttaat cagatcggtc agtttctgtt
   114241 tttcaattaa cgccgcacgc gccaaatcct ctctctcttt cagcagcgcc agttcggctt
   114301 tttcctgcca ttcaacttca cgcgccgacg cttgttcaat acggcgagtc agctgtttct
   114361 tttctgccag cgcacgcgcc gaagtagaac gtacttcaac caatgtatct tccatctcct
   114421 ggatcatcag acgcaccagt ttctgtggat cttccgcttt ctctaacaga gcgttgatgt
   114481 tggcattcac gatgtcggca aagcgagaaa aaatacccat aatgttgtcc tcttgatttc
   114541 tgcattcagg attgtcctgc tgaactgtta atacaatttg cgtgccaatt ttttatcttt
   114601 ttgatttata aagatctgat tgaagaatca acagcaacat gccaggatga attagctaat
   114661 tacactaaca agtggcgaat ttcatcatgg cagaatacaa agataattta ctcggtgagg
   114721 cgaacagctt tctcgaagtg ctggaacagg tttcgcatct cgcaccgctg gacaaaccgg
   114781 tgctcatcat cggcgaacgc ggcaccggta aagagctgat cgccagccgc ctgcattatc
   114841 tctcctcccg ttggcaaggg ccgtttattt cccttaactg cgcggcgtta aatgaaaatc
   114901 tgctggattc cgaactgttt ggtcacgaag cgggggcgtt taccggtgcg caaaaacgtc
   114961 atccagggag atttgaacgt gccgatggcg gtacgctatt tcttgatgaa ctcgctacgg
   115021 caccgatgat ggtgcaggag aaattattgc gcgtgattga gtacggtgaa ctggagcgcg
   115081 ttggcggcag ccagccgttg caggtgaatg tgcggttggt atgcgcgacg aatgccgatc
   115141 tcccggcgat ggtcaatgaa ggtacttttc gcgctgacct gctcgaccgg ctggctttcg
   115201 atgtagtgca actgccgcca ctacgcgaac gtgaaagcga cattatgtta atggcggaac
   115261 actttgccat ccagatgtgt cgggaaatca agctgcctct gttcccgggg tttacggagc
   115321 gcgccagaga aacattgctg aattatcgtt ggccaggaaa tattcgtgaa ttgaaaaacg
   115381 tggtggaacg ttcagtgtat cgccacggca ccagcgatta tccgcttgat gacatcatta
   115441 ttgatccctt taaacggcgt ccgcctgaag acgctatcgc cgtttcagaa accacctcgc
   115501 ttccaacact gccgctggat ttacgtgagt ttcagatgca gcaggaaaaa gagttgctgc
   115561 aactcagttt gcaacaaggg aaatataacc agaagcgcgc agctgaattg ctgggcttaa
   115621 cctatcatca gttccgcgcg ttgttgaaaa agcaccagat ttagcgaagt tgggcaatac
   115681 aacttgccgg atgcggcgtg aacgccttat ccggcataca taatcaatac gtcgtttgca
   115741 ggtctgataa gcgcagcata tcaggcatta ttgcgttgcc cctgcttctg cgtattaatc
   115801 tctcagcgca tcctgcaaaa ttttcattgc cgcatcgaat tgcgcatccg ggatggtcag
   115861 cggatacagg aaacgaatca cgttgccgta tgcgccacag gtcagtagaa gcagtccctg
   115921 cgccagcgcg cgttgctgaa ttttttgcgc aatggcggct gacggctcgc ccgtttgcgg
   115981 gtcgttaaat tctgccgcaa tcatcgagcc cagaccacgt accgccgcga tggccggaac
   116041 gttttctttg gcatcaatca gggttttcgt cagacgctgc cccaactgca tggcgcgctc
   116101 acagagtgac tctttgtcga taatgttgag caccgcgtgc gcggcagcca ccgccagcgg
   116161 attcccggcg taggtgccgc caagcccgcc cggcgcgggc gcgtccataa tattcgcgtt
   116221 accgaccaca cccgaaagcg gcatcccgcc cgcgaggctt ttcgccatcg tcattaagtc
   116281 cggcttatcg gcgtaatgat ccatcgcaaa cagtttgccg gtacgcgcaa agccgctttg
   116341 cacttcatcg gcaatcatca caataccgtg ctcgtcgcac aggcggcgaa tggcggcaac
   116401 cagctctttt ggcgcaacgt tgaaaccgcc ctcgccctgc accggttcga aaataatggc
   116461 cgcgacctgc ttcgcttcga tgtctgattt aaacaagcgt tcgatggcgt cgagggagtc
   116521 ctgtgttgaa atgccgtgta aatctgacgg ataaggtacg tgatacaccg aaccagggaa
   116581 cgggccgaag ccgattttgt acggcgcgac ctttccagtt aacgccatag tcatatacgt
   116641 acgaccgtgg aagccgccgc taaacgcaat cacgccaggg cgtccggtat gggcgcgggc
   116701 gattttcacc gcgttttcca ccgcttccgc accggtggtg aagaacgcgg ttttggcctg
   116761 cccgctcacc ggggcaaggg cgttgatttt ctccgccagg gtgacgtagc tttcatatgg
   116821 cacaatctga tacgcggtgt gggtaaactg ttgcagctgc tgctccaccg ccgcgaccag
   116881 atcagggtgg cgatgtccgg tattcagcac cgcaatgcct gcggcgaaat cgatgtactc
   116941 gttgccctca acatccttca gcgtggcgtt ttcagccgac tgggcgaaga agttacacat
   117001 cacgccaacc ccgcgcgggg tggcagaaag acgacgctga tggaattcat tgttgctcat
   117061 gtttcacacc ttttgactct tcactgttaa tcggaaatta aacgcccaga cgatcgcgca
   117121 ggctgtaata cgccgcgccc atcgcggtaa acggtacgcg cagcgtgcgc ccgccgggga
   117181 acgggtaatg cggcagattg gcgaaggcat cgaaacgttc ggcgtcgccg cgcagcagtt
   117241 cggcaatcaa acgtccggcg agatgagtac aggtgacgcc gtggccgctg taaccctgca
   117301 tgtaatagat gttggtatcg aggcgaccga actgcggcat acgcgacagg gtcagcagga
   117361 agttgcccgt ccagcgatag tcgattttca cgcccttcaa ctgcgggaag gttttcagca
   117421 gtttcggcac cacaagacgc tcaacgtcat ccgggtcgcg cgcgccgtac accacgccgc
   117481 cgccgtacag caaacggttg tcggcggtaa gacggtagta atcaagcaga taattacagt
   117541 cttccacgca gtagtttttc gggatcagcg aacgggctaa atcatcggaa agccgctcgg
   117601 tggtgatcac ctgcgtaccg cacggcatgc tgcgtttcgc cagttccggc tccactttat
   117661 cgcccaggta cgcattcccg gcgacaatca catacttcgc cgtcacctga cctgtggctg
   117721 tacgcactac ggcgggcgtg gtgtgctgga tctgcgtcac tgcggaaagc tcatacacgc
   117781 gcccgccgtt caggcggata gcgtccgctt cgccaatggc gaggttcagc gggtgaatat
   117841 gcccgccgct gtgatccagc agcgcaccag tgtagcgatc gctggcgacc tcgcggcgaa
   117901 tggcgttggc gtccagcatc tccagctgtt tattgccgta gcgttcccag ttctctttct
   117961 gctcttcaag tgtggcgagc tgtttatcat tcatcgccac aaacagaccg ccggggcggt
   118021 agtcgcagtc aatctgataa cgtttgatac gttcgcggat gatctcacca ccttcgaaca
   118081 tcatgctgcc gagcatacgg gcggtgtcca tgccgtagct tttttcgata acgtcgatgt
   118141 cgcggctgta ggagttcaca agctgcccgc cgttgcgccc gctggcgcca aagccgatgc
   118201 gtgaggcttc cagaaccacc acgtcaaagc ccgcttccgc cagatgcagt gcggaagaca
   118261 gcccggtata gccgccgcca accacgcaaa cgtcgcagga aattgactca ttcagcgtgt
   118321 cgaatggcgc gtatttattc gcactggcgg cgtagtaact gctggtatgt tctgtcattt
   118381 caggcctcca ggcttatcca gatggttttc agttcggtga atttttcaag ggcatgcagg
   118441 gatttgtcgc gaccgttgcc gctctgctta tagccgccaa acggcacggt catatcgccg
   118501 tcgttgtagt tattgacgaa gacggaaccg gctttcaggc gtcggctcat gcggtgcgcg
   118561 cgggagaggt cgcgcgtcca taccgccgcg ccaaggccgt actggctgtc gttggcaagc
   118621 tgtagcgcct gttcttctga tgtgaaacgc gtgaccacca gcaccggacc gaaaatctct
   118681 tcgcggctta aggacgcatt cgggtctaca tccacaatga tggtcgggcc gatggcgaca
   118741 gccagcccgg cgttacggcc atccaacaac agttgccctt tgctttcgcc ttcctgaata
   118801 aagctatgga ccgagtcggc gtgggcgcag tcgattaagg tgcccatggt ggttgcggga
   118861 tcaagtggat ggcccggctg ccagttttgc gcctgctgtt ttaacagggc taagaattca
   118921 tcggctatgc tctcttccag caacaggcgc gttccggcga tgcacacctg cccctggttg
   118981 tagaagatgc ctgctgcggt ggcgcttgcc gcctgttgca aatccgggca gtcagcgaaa
   119041 acgatgttgg cgcttttgcc gcccgcttcc agccagacgc gtttcatgtt gctgtcgcct
   119101 gcatctttca gcagctgttt cccggtacgg gtcgaaccgg taaaggcaat ggcgtcgata
   119161 tcgttatgac gcgacagcgc ctgccccgct tcatgaccaa aacccgtcac cacgttcaac
   119221 acaccatccg gcaagcctgc ttctttcgcc agccccgcga gacgaatcgc actgagcggt
   119281 gatttttcag acggtttcag gacaacgctg ttccctgctg ccagcgccgg gccaagcttc
   119341 cagcaagtca gcagcagcgg gaagttccac ggcacgatgg cggcaatcac gccgaccggt
   119401 tcacgcacga tcatcgccag ctcatggcta ctggtggtcg ccacttcgcc atacactttg
   119461 tcgatcgctt cggcgtacca gcgaatggcg cgcgccgcgc cgggtatatc atcacgcaga
   119521 ctgtgacgaa tcggtttgcc ggtgtcgaga gtttccagca gtgccagctc ttcggcgttg
   119581 gcttctatta aatcggcgag tttattcagc accgctttac gttttgccgg agaagagagt
   119641 gaccagtcgc cgcgttcaaa tacgccgcgt gctgcgctca ccgcacggtc gatatcgacg
   119701 ctcttgccgc gcgcaatatt cgccagcggt gcctgggtaa ccggatcaac ggtttcaaag
   119761 gtttcatttt ccgccgcagc agtatattca ccgttaataa ataagcggtt ttcaatggcg
   119821 agacttaacg ctttatcctg ccagtaagcc agatgatgaa aattcattat gactcctgtt
   119881 tcacgtctat cagatatatg cagtttgtga acgtcagggc gcagagatcc ccgggccgta
   119941 tgcccgttta tttctggcga cattctttat ggattgccgg gcgcattgcg cttacccggc
   120001 ttacagaaca aaaagattaa aacgtggtgg gcgtatgggc gctgataatt cggcaaatac
   120061 ctgccgacgt attactgaaa ctgtgcggga tgccggtatt aatggcataa ctttgccccg
   120121 cgacgaggtg gtaatcctga ccattaatcg tcagaataat ttcaccttcc agtacagtgc
   120181 ctatttcctc accctgatgc ttaattcttt ccccagtggt tgtgcccggc tggtacgttt
   120241 caaagatcat cgccagcgtg cgattcgggt taccgttatg aaccagcttc attgacacac
   120301 cctgactgcc catctcaatt aaatcgtcct gattaatgac gacctgcggc tcatcaggtt
   120361 tttccggctc ggaaaagaat tccgagagtg acagaccata caccttcagc agcttttgca
   120421 gcgtactgat ggcagggctg actttatctt gttctatcgt actgatagca ctgtgagtca
   120481 gcccggagag ttcggcggca cgacgttgtg aaagcccctg ctgctggcgg atttccgaca
   120541 agcgttttcc tggcgccagt ccctcatcac tcatatttgc atttccttaa actgtagtgg
   120601 tcagagtcgc tgtttttcag cgatatggtg ctgacaagcg gtgataaagc cctcgaacaa
   120661 tatacgcgaa agcgcgtact cgctactgtt ccattccggg tgccactgta cgcccagcgc
   120721 aaaaggatga ttgatgacac taaccgcctc aactaagcca tccggcgagc gagcttcaac
   120781 acgcaaccgt gggctaacga ccttcgcccc ttgtccatgc agagagttta cccaaaagtt
   120841 gctacattca ggtaacaatg cagacagcaa tcccccctct tcaacctgaa cttcatgcga
   120901 cggtgcatat tgctgttcca ccggcagttc gggatcttcc cgatgttcca gcaattcagg
   120961 ctgttcgcac agcttgcgat gcaacgaacc accggttgcc accaccagtt cctgtaaacc
   121021 ccggcagatg gcgaaaatgg ggatgcgcct ttcgagtgcg gcattaatta acgccatgct
   121081 cagaagatca cgctcgggat cggcgtcagg ctcatcgccg ttttcaccat ataggtgcgg
   121141 ctgcacattg ctgggactac caggaaggta aatgccatcg agtttcggca aaagttgttc
   121201 aagtaacgac ggttccgcca gcgcatgtgg cagcgcaatt ggcaagccgc ctgcgtggat
   121261 gatggcattc aggtactttt cttgcagagt ctgggtcgca tgccccttaa gcctgttcct
   121321 gcacattacg acaccgataa ccggattgtt cattatattt tccattctca atctcacaaa
   121381 gtggactaaa ttatcgccat tactgcggtt taaatgacct ttatgttcaa tattttttca
   121441 atctagcagt gggttttcat ttttgcaaac tcaatttaac atttgacaaa catttagttt
   121501 gcatacagat tcgaatggtg gtcattatat tttacgcttt gataacgagc ggaaaacaaa
   121561 ccaaaggcga agaatcatgg aaaccaatat cgttgaagta gagaactttg ttcagcagtc
   121621 agaagagagg cggggtagcg cctttacgca ggaagtgaaa cgctacctgg agcgctaccc
   121681 gaatacgcaa tatgttgatg ttttacttac cgatttgaat ggttgcttcc gtggaaaacg
   121741 tattcctgtt tcaagcctga agaagctcga gaaagggtgt tatttcccgg cctcggtatt
   121801 tgcaatggat attctgggca acgtagtaga agaagcaggt ctgggtcagg aaatgggcga
   121861 gccggatcgt acctgtgttc ctgttctcgg ttccttaacc ccttctgccg ccgatccaga
   121921 gtttatcggt cagatgctcc tgaccatggt cgatgaagat ggcgctccct ttgacgttga
   121981 gccgcggaac gttcttaacc gcctgtggca gcagctgcgc cagcgcggat tgttcccggt
   122041 cgtagcggta gagctggagt tctatttact ggatcgccag cgcgacgctg aagggtatct
   122101 gcaaccgccc tgcgcgcccg gcactgatga ccgcaataca caaagccagg tttactccgt
   122161 tgataacctc aaccacttcg ccgacgtgct caatgatatt gatgaactgg cgcagttaca
   122221 gctgattccg gcagatggcg cggtcgctga ggcctcgccg ggtcagtttg aaatcaacct
   122281 ttaccatact gataacgtgc tggaagcctg cgatgatgcg ctggcactaa aacgcctggt
   122341 gcgtctgatg gcagaaaagc ataagatgca cgccactttt atggcgaagc cgtatgaaga
   122401 gcacgcgggc agcggaatgc atatccatat cagtatgcaa aataatcgtg gcgagaacgt
   122461 gctttctgac acagaaggcg aagattcgcc gctgctgaaa aagatgctcg ccgggatgat
   122521 tgacctgatg ccgtcatcaa tggcgttgct ggcaccaaac gtgaactcgt atcgccgctt
   122581 ccagccgggg atgtatgtac cgacgcaggc gtcgtggggc cataataacc gtaccgtcgc
   122641 cctgcgtatt ccatgcggcg atcgtcataa tcaccgcgtg gaatatcgcg tggcgggtgc
   122701 cgatgccaac ccatatctgg tgatggcagc gatttttgcc ggtattttgc atggccttga
   122761 taacgagctg ccgttgcagg aagaagtcga aggtaacggg ctggaacagg aaggcttacc
   122821 cttcccgatt cgccagagcg atgccctggg tgagtttatc gagaatgatc acctgcgccg
   122881 ctatttaggc gaacggtttt gccatgtgta tcacgcctgt aaaaatgatg agctgttgca
   122941 gtttgagcgc ctcatcacag aaacagaaat tgagtggatg ttgaaaaacg catagtaccc
   123001 cttgcggggt tgtttcatcg gcctgagcgg agccaggcct gacatttgct ggtaatccag
   123061 acatttcccg gatgtcgcgt tatggagcgc aggcagcaac gggcttccgt taacgattaa
   123121 gactgtgacg aggaaatgag atgatgccga tgattaaatc cccgcatggc gcgggaggct
   123181 gcgtatgcgc ccctcctgcg actgactgga ctccgccccc tttgcttccc ttgctaaaca
   123241 ggtttgattt caggtcgaca cgaccgcaaa ccttattacg caggggaggc agcaattatg
   123301 gctattaatt caccactgaa tattgctgcg caacccggca aaacccgtct gcgaaaatca
   123361 ctgaaattgt ggcaggttgt gatgatgggt ctggcctatc tcacgccgat gactgtgttc
   123421 gatacctttg gcattgtatc tggcattagc gacggtcacg tccctgcgtc ctatttgctg
   123481 gcgctggcgg gcgtgctgtt taccgctatc agctacggca aactggttcg ccagtttccg
   123541 gaggccggtt cggcctatac ctacgcgcaa aagtcgatta acccgcacgt cggatttatg
   123601 gtcggctggt catcactgct ggattatctc tttttgccga tgatcaacgt tttgttggcg
   123661 aaaatctatc tctccgccct cttcccggaa gtgccgccgt gggtgtgggt ggtaaccttc
   123721 gtcgccattt taaccgccgc gaatctgaag agcgtcaacc tggtcgctaa cttcaatacc
   123781 ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta tcttcctggt ggttcaggga
   123841 ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc agccgtttat cagcgagaac
   123901 gcgcacctga tcccgattat taccggggcg acgattgtct gtttctcgtt cctcggtttc
   123961 gatgcggtga ccacactttc ggaagagaca ccagacgccg cacgcgtgat cccgaaagcc
   124021 atcttcctga cggcggtcta tggtggcgtt atctttatcg cggcgtcgtt ctttatgcag
   124081 ctgttcttcc ccgatatcag ccgctttaaa gacccggacg ccgcactgcc tgaaattgca
   124141 ctctacgtcg gcggtaagct gttccagtcg attttcctct gcaccacgtt tgtgaacacg
   124201 ttagcgtctg gcctggcctc acatgccagc gtgtcacgtc tgctgtatgt gatggggcgc
   124261 gacaatgtgt ttccggagcg cgtgtttggc tatgtgcacc caaaatggcg gactccggca
   124321 ctgaacgtca ttatggtcgg aattgtcgcg atgtcggcat tgttctttga tttagtcacc
   124381 gcgacagcat tgattaactt cggtgctctg gtggcgttta ccttcgtgaa tctgtcggtg
   124441 tttaatcatt tctggcggcg taaaggaatg aataaaagct ggaaggatca cttccactat
   124501 ttgctgatgc cgctggttgg cgcgctgacg gtgggtgtgc tgtgggttaa cctcgagtca
   124561 acgtcactga cactcggtct ggtatgggct tcgttaggcg gcgcatattt gtggtatttg
   124621 atccgccgct atcgcaaagt gccgctgtac gaaggtgaca gaacgccagt gagcgaaacg
   124681 taatagtcgg atgcgcgccg cattatcctg cctgagggta atgcgccgcg ccattaacta
   124741 taaaaagatg tatgacccgg atcacgagtg ctatcatccg cccttatgct tagcgcatta
   124801 accccagggg caaataatga accacgatat cccgctaaaa tattttgata ttgccgatga
   124861 gtacgcgacc gaatgcgcag agcctgtcgc agacgctgaa cgcacaccgc tggcccacta
   124921 cttccagtta ctgctcaccc gtttgatgaa caatgaagag atcagcgaag aagcccagca
   124981 tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt gatgagatcg cagagttttt
   125041 gaatcaatgg ggtaatgagt aattatttaa ttgcagacga tcggtcagta tcgttgtccg
   125101 gataaagcgt tcacgccgca tccggctaac aatgacacgc actgcagcgt gctttaatgg
   125161 gtttctcacg atcgtaccgc tctgctcctc gctaagttgt tctgtgagtt atagttatag
   125221 agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca tctgttcaac gaccaaccac
   125281 acggccctca ttacgacccc tgaacacaaa cggtaatgga aaatgtcatt aaatattagt
   125341 aagatagcct ctttacataa aatcccttaa tattatgcgc caggtattat cgtctctttt
   125401 ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg cctgaatccc ccccgcatgc
   125461 tgatatccgc gacagcggtt ttgtctattg cgtcagcggg caagtcaaca cctttaaccc
   125521 atccaaagcg agcagtgggt taattgtcga tacccttgcc gcccagtttt atgatcgact
   125581 gctggatgtc gatccctata cctatcgcct gatgccagaa cttgccgaaa gctgggaagt
   125641 actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc gatgtcccgt ttcaaaaaac
   125701 cgcctggttt actcccactc gcaaaatgaa tgccgacgat gtggtgttta ccttccagcg
   125761 aatttttgac cgcaacaacc cgtggcataa cgtcaacggc agcaacttcc cctacttcga
   125821 cagcctgcaa tttgccgata acgtgaaaag cgtccgtaaa ctggataatc ataccgttga
   125881 gttccgtctg gctcagccgg atgcttcttt tttgtggcac ctcgcaaccc attatgcttc
   125941 ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa gatcgccagg agcaactcga
   126001 ccgtcaaccg gtcggcactg gaccgtatca gttgtcggaa taccgcgccg ggcaatttat
   126061 tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg ttaatgccgc aggtggtggt
   126121 ggatttaggc tccggcggca ccggacgtct gtcgaaactc ctgaccgggg aatgcgacgt
   126181 tctggcctgg cctgctgcca gccagctatc cattttgcgt gacgacccgc gcttgcgttt
   126241 aacgctgcgt cctgggatga acgtcgccta tctggcattt aacaccgcca aaccgccgct
   126301 aaataatccc gctgtccgcc atgcgctggc actggcgatt aataaccagc gcctgatgca
   126361 atccatctat tatggtacgg ctgaaacggc ggcctctatt ttaccgcgcg cctcgtgggc
   126421 ctatgacaac gaggctaaaa ttactgaata caatccggcg aaatcgcgcg aacagttgaa
   126481 gttgttgggg ctggaaaatt taacgctgaa actgtgggtg cccacacgtt cgcaggcgtg
   126541 gaaccccagt ccactgaaaa ctgccgaact gattcaggcg gatatggcgc aggttggcgt
   126601 aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag gcgcggttga tggatatgag
   126661 ccatgatctg acgttatccg gttgggcgac ggacagtaac gacccggaca gtttcttccg
   126721 tccgttactg agctgcgcgg caattcattc acagaccaac ctcgcccact ggtgcgatcc
   126781 gaaattcgac agcgtgttgc gtaagacgct ctcctcgcag cagctggcgg cgcgtattga
   126841 agcctatgac gaagcgcaga gtattctggc gcaggaattg cccattttgc cgctggcgtc
   126901 gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt ctggtactta gcccgtttgg
   126961 taacgcctcc tttgctgggg tgtatcgcga gaaacaggat gaggtgaaaa aaccatgatt
   127021 atcttcacct tacgccgcat tttgttattg attgtcacct tgttcctgct gaccttcgtt
   127081 ggcttcagct tgagctattt cactccacac gcgccgttac aaggcgcgtc actgtggaat
   127141 gcctgggtgt tctggtttaa cggcctgatc cactgggatt ttggtgtttc cagcattaac
   127201 ggtcaaccaa ttgccgaaca gttaaaagag gtcttcccgg cgacgatgga actgtgcatc
   127261 ctcgccttcg gttttgcgct gattgttggc atccccgtgg ggatgattgc cgggattacg
   127321 cgccataagt ggcaggataa cttgatcaac gccatcgcct tactgggctt ttcaatcccg
   127381 gtgttctggc tggcgcttct gttgacactg ttttgttcac tcacgctagg ttggttgccc
   127441 gtttcagggc gtttcgatct gctctacgaa gtgaaaccga ttaccggttt tgcgttgatt
   127501 gatgcctggc tttcggactc accatggcgg gatgaaatga tcatgagcgc aatccgccat
   127561 atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa ctgaagtgat ccgcctgatg
   127621 cgtatcagca ctatcgaagt gtatgaccag aactatgtaa aagcggcggc gacccgcggt
   127681 ttgtcgcgct ttactatttt gcgtcgccac gttctgcata acgcgttacc gccggttatt
   127741 cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg cgatgatcac cgaaatggtt
   127801 tttagctggc cgggtttagg gcgctggtta attaacgcca tccgccagca ggattacgca
   127861 gccatttccg ccggagtgat ggtgtgtggc tcactggtca ttattgttaa cgtgatttct
   127921 gatattttgg gtgccatggc taaccctctg aaacataagg aatggtatgc cttacgatag
   127981 cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc gcctggcgca aattttatag
   128041 tgacgcctct gcgatggtcg gcctgtacgg ctgcgcgggg ctggctgtac tgtgtatgtt
   128101 tggcggctgg tttgcgcctt acggcatcga ccagcaattt ctcggttacc aattattgcc
   128161 gccatcatgg tcgcgctatg gcgaagtttc tttcttcctg gggactgacg acctcggacg
   128221 cgatgtttta agccgtttac tgagcggagc tgcgccaacc gtgggtggcg catttgtggt
   128281 gacacttgcc gcgacgatct gtggcctggt actgggcacc ttcgccgggg cgacgcacgg
   128341 cctccgctca gcggtgctta accatattct ggatacctta ctggcgatcc cttccctgct
   128401 gttggcaatt atcgttgtag cgtttgccgg accgagtttg tcccacgcta tgtttgctgt
   128461 ctggctggcg ctgctgccgc gtatggtacg ttcgatttac agcatggtgc atgacgaact
   128521 ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca tcaacgctga atattctctg
   128581 gtttgctgtg atgcctaaca tcaccgccgg gctggtcacc gagatcaccc gcgcactgtc
   128641 gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat cttggcgcac aactcccctc
   128701 gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg atttatgtcg ccccgtggac
   128761 tgtcatgctg ccaggtgcgg caattatgat tagcgtgctg ttggttaacc tccttggcga
   128821 cggtgtccgt cgtgcaatta ttgcgggggt ggaataatgc cattactcga tattcgtaac
   128881 ctgactattg aatttaaaac cggtgatgag tgggttaaag ccgttgaccg ggtaagtatg
   128941 accttaaccg aaggtgaaat ccgcggtctt gttggtgaat ccggttccgg caaaagtttg
   129001 attgcgaaag cgatttgtgg ggtgaataaa gataactggc gtgttactgc agaccgtatg
   129061 cgttttgatg atatcgattt gctgcgtctc tccgcacgcg aacggcgcaa actggttggc
   129121 cacaacgtgt cgatgatttt ccaggaaccg cagtcgtgtc tagatccttc agaacgtgtg
   129181 ggtcgccagt tgatgcaaaa catcccagcc tggacctata aaggccgttg gtggcagcgt
   129241 tttggctggc gcaaacgccg tgccattgaa ctgctgcacc gcgtgggaat taaagatcat
   129301 aaagatgcga tgcgcagttt tccctatgag ttgaccgaag gtgaatgtca gaaagtgatg
   129361 atagccattg cactggcgaa tcaaccgcgt ctgctgattg ctgacgaacc gacaaactca
   129421 atggagccaa caacccaggc gcaaatcttt cgcctgctga cgcgtctaaa ccaaaacagc
   129481 aataccacta ttttgcttat cagccatgac ttacaaatgc ttagccaatg ggcggataaa
   129541 attaacgtgc tttactgcgg tcaaacggtg gaaaccgcgc cgagtaagga gttagtgacg
   129601 atgccacatc atccttatac ccaggcgctg atccgcgcga taccagactt cggaagcgcg
   129661 atgccgcata aaagtcgcct caatacgcta cccggcgcta tcccactgct tgaacagtta
   129721 ccgattggtt gtcgtctggg gccacgttgc ccgtatgcac aacgagaatg cattgtgacg
   129781 ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc atttcccgct gaacatggag
   129841 aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat ttaagtaaaa cgttccgcta
   129901 ccggaccggt tggtttcgtc gtcagaccgt agaagcggta aaacccttga gctttacgct
   129961 acgtgaaggc cagacactgg cgattattgg cgagaatggt tcgggtaaat ccacgctggc
   130021 aaaaatgctg gcgggaatga tagaacctac cagcggcgag ttattgattg acgatcatcc
   130081 actgcatttt ggcgattatt ccttccgcag tcagcgtatt cgcatgattt ttcaggatcc
   130141 ttcgacctcg ttgaatcccc gtcaacgtat ctcgcaaatt ctggattttc cactgcgcct
   130201 gaacaccaac ctggaaccgg aacagcgtcg caaacagatt attgaaacga tgcgtatggt
   130261 tgggctgcta ccggatcacg tcagttacta tccgcatatg ttggcccccg ggcaaaaaca
   130321 gcgtctgggt ctggcgcgcg cgctgatatt gcgcccaaaa gtcattattg ccgatgaagc
   130381 cctcgcttca ctggatatgt cgatgcgttc gcagttgatt aatctgatgc tggaattaca
   130441 ggaaaaacag ggcatttcgt atatttatgt tacccagcat atcggaatga tgaagcacat
   130501 tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc gagcgaggca gcaccgcgga
   130561 tgtgctggca tcgccgctgc atgaactcac caaacggctg attgccgggc actttggcga
   130621 agcattgacg gcggatgcgt ggcgtaagga ccgttagtgt tctgcacctc atcctgacag
   130681 gggaaccccc ctcaggatga ggtattcatt acttttgatt gtcgattctg gtgagagcaa
   130741 ccaggaatgc cagtgtataa cacaccgtaa ttgccagact catttttaac agtgtttctc
   130801 cgccaaatcc cgacaatggc gcggctatac cgccgaagac aaacatgctc atccccatca
   130861 acgcagacgc cgttccagat tcctgcgtcc ctactgcact catagcctct gccccgccaa
   130921 cagtgccgat gccgctacaa aatgcaatag tgaaaaataa tgccaccagt gccggaatgg
   130981 gcaattgtgt ccatgcgcat aagaccgtga gcaatgcaca caaaattgcc gctatcaggc
   131041 cacctcgcaa caatgtcatc gcattaatcc gtcgcgccag acgcgaaaag atccatgaag
   131101 caatgataag tccgatgccg ttaaggccaa acaccaggct aaattgcatt ggactaaagc
   131161 caaattcctt ctgcaacacg aacgacgaag agccgatata cgcaaacaag cctgccagca
   131221 taaaactttg aatcaggcaa aagcgcataa aacggcggtt ctgcaccacg cttcgtccgg
   131281 ttaatagcaa tgatgagccc cttttatttt ctggcaaggt ctcattaata aataacacgc
   131341 agcccagcaa caatacggtg ctaatttcag ccattaccca gaataaagtg cgccagtcaa
   131401 aagtgctaac aatgtacccg cccagcactg gcgacaacac cggtgccagg ccattcaccg
   131461 tcattaacag cgcaaaaaac tgggtcaacg ttactccctg atatttgtca cgagcaatag
   131521 aacgagagag caccgaacca cccgcccccg cgatcccttg aataaaacgc cagaccacca
   131581 gccagtaaat attgttcgtt gtcgcgcaca aaatggaaga aacaataaac aatagcaacg
   131641 acaagattaa cggacgttta cgccctattt tgtcgctcag agggccaaat aacagttgtc
   131701 cgacgccaag accgatcagt gatgcagtca gcgttaattg cgttatggtc gtggttgccg
   131761 ccagttgctc agacatctcc ggcaaagccg gcaaataaag atcggtacac atcgggccga
   131821 taccggctaa aagaccaaga atcaatgccc atgaaagaga gactctagcc atattattcc
   131881 ttacttgagt gatgacatgc cgccgcccag cgactgccag agcgtgattc ggtttgtaaa
   131941 atcagtcagt tgcagtgcca gcagctcctg ctgtgcgctc cacaagctgc gctgcgccgt
   132001 caacacggtc aggtaatcac caacacccgt ctgatagcga cgcaagccaa catcgaccgt
   132061 ttgttgctca gctttaacat actggcgctg tgcatccagt tgctcttcaa gcgtggtccg
   132121 ccgcgccagt gcgttagcga catctttaaa tgctctctga acggtttttt cataggtggc
   132181 aatgagtcca cgcttttgtg cttctgcgta acgaagctgc gccagattac ttccgccagt
   132241 gaacagcggt aatgtgaccg atggcgtaaa tgaccagatc tgcattccgt ggctgaacaa
   132301 cgacgacaat gcgtcactgc caacaccagc gctggcagtc agcgaaatcg tcgggaaaaa
   132361 gttagcccgc gccgcgccaa tatcggcatt ggcgctcttc aaattatgtt ccgcttcctg
   132421 gatatccggg cgacgcagca gaacgtcaga tgacactccc gcaggaacca gacttatcat
   132481 ctgttccggc aggctttcca gtgttcccgg tagcaaattt tcctcaagcg ttgtacctgc
   132541 cagtaagttc agcgcatttt tatcctgcat cacttgtgtc tgatagctgg caacgctggc
   132601 gcgcgcttgc tgataaacac tcatcgcctc actgacatct gtcgctgccg ccgtgccaac
   132661 ctgttgctgg cgctggataa ttttcaatga gttttctgca ctggtcatcg tttcttttgc
   132721 cagggccagg ttgctgttat cggccgccag tgtcagccag gcggtgctga tttcagcaat
   132781 cagcgttaaa cgggtgttct gggctgtaaa ttcactggca agccaggttt ctcgcgccgc
   132841 ccgagataaa ctctgattac gaccaaacag atctaacgta aaactggaca ccgtgccgtc
   132901 agcctcggca gtggtttccg taccattcgc cagcgaacga ctgcgggttg aacttaaggc
   132961 cgcattcacc gtcgggaata acgacgcatt agtttgccca tagagcgccc gtgctgagtc
   133021 aatatcagca atcgcttttt gcacatcgcg gttactgttc agtgcaatag tcaccacctg
   133081 ctgcaatcgg ggatcgtgga tcacctgctg ccagtcatgg ctgattgcct ttccctggcc
   133141 ctgtgctccc ggcaaggtcg ccgggattgg ggattcaggc gtgctgtagt ggggatcgag
   133201 cgagacacat cccgcactca acagcaccag aaaaataaga gaacgacgca acataactta
   133261 ctcctgacgg cgcgatttac cggcaaacaa acgcttcacc agtacaaaaa acagaggaac
   133321 aaagaaaata gccagcaacg tagcggtcag cgtaccgcca ataatgcccg taccaatggc
   133381 gatgcggctg ttcgcccctg agccggttgc tatcgccagc ggcattaccc cccgcaataa
   133441 acgccagcga ggtcatgatg attgggcgta aacgagtctg cgccgcgcgt aatgccgcac
   133501 gactcaagga ataaccctct gcaaccgccg cttcagcaaa ttccacaatc agaatggcgt
   133561 ttttcgacga caagccgata gtggttaata gcgccacctg gaagtaaaca tcgttgttta
   133621 aatcacgcat ccaggccgcc agcgccgcgc caagaagccc cagagggatc accagaatga
   133681 ccgagaacgg gactgaccag ctttcgtaca gtgctgcaag gcagaggaaa accaccagaa
   133741 tagaaactgc atacagactc aatgcctgac cgctggccag tttctcctgc aatgacaaac
   133801 cactccaggc ccaggttgtt cccgcaggaa gactgtttgc cagttcctcc atttttgtca
   133861 ttgcatcgcc ggaactaaag ccagtcgcat tttccccctg aatttcatag gctgcagaac
   133921 cgttgtagcg caccagtctt tccggtccat acagccagcg ggtggtcgca aaagcagaga
   133981 atggtgtcat ggcgttatca ctaccgcgca caaaccattt acctaagtct gacggcgcgg
   134041 aacgaaattc actgtcgccc tgaatgtaga cttttttcac acgaccacgg tcaataaagt
   134101 cattcacata agtaccgccc cacgcactgg acagggtgtc ggtgacatca ttcaaactca
   134161 accctaatga caccgcttta ttactatcaa tatctacctg caattgcggc atttgtggca
   134221 aatcattggc acgaacagaa tgtaactccg gactttgatt cgctttttct atcaactgat
   134281 tacgcatttg cagtagtgtt tcgcgatcgg ttccaccgtt agctaacaat tcaaacgtaa
   134341 aaccattgct ttgccccagc ccatcaacgg ctggcggcgt catcgcaaat accgtggcat
   134401 cacgaattgt gcccagctct ttggttgccc gtagggcgat agcctgggcg gtgttttctg
   134461 cccctttacg ttgagaccag tttttcaacg aaacaaacgc catcccggtg ttctgtccgc
   134521 tgccgctaaa actgaaacca tcaacggtaa aaatgacatc ggtatttgct ttctcgttaa
   134581 tcaggaacca gtcaacaatc tggcgattca cttctgctgt acgggcagcg gtcgccccgg
   134641 ccggcagcgt gtactgcacc atgatttcgc cctggtcttc agtgggtaag aaactgcccg
   134701 gcagtttcca catcatcagg gccattccac cacccagtac gagataaagc cccatcgttc
   134761 gagcagcacg gcgcaggaca taaattacgc ctcgctgata tttatcttca gtacggcggt
   134821 aaaagcggtc aaatgcgccg aaaaagcctt ttttatgtgg cggaacatgc tggaggacgg
   134881 aaccgcacag ggcgggagtc aaggtcaatg ccaccaccac ggaaagcagc attgcggaga
   134941 taatggttat cgaaaactga cgataaatta cccccgtaga tcccccaaag aaggccatag
   135001 gcaggaatac ggctgacaac accagcgcaa tggcaaccag tgcgccagaa atctcgccca
   135061 ttgatttttc cgtggcttca cgcgcgggca accctttgtc acgcataatg cgttcgacgt
   135121 tctccactac cacaatggca tcgtccacca gcaagccgat cgccagcacc atagcaaaca
   135181 acgtcagggt attaatcgaa tagccaaaca acgcaagaac gccgaatgtg cccagcaaaa
   135241 cgacggggac agctaacgcc ggaatcagtg tggcacgcag gttttgcagg aataaataca
   135301 tcacgcaaac caccaatacg atggcttcaa acagcgtctg aattacgtcc tctacagaga
   135361 ttttaataaa ttcagtgctg tctttcgggt aagcgatgtc gtatccctgc ggcatgttcc
   135421 gctggaattc ggcaatctta tccttgacca gcgtcgccgt attcagcgca ttcgcaccgg
   135481 gcgaaagcat tacagcaacc ccggcagacg ggtgaccgtt aagtttgccg atagcggtat
   135541 aatcttcact gcccatctca acgcgagcca catcttttat acgaacaact gcgctgtctg
   135601 actggctttt cacgataata tttttgaact gatccaccgt ctgcaagcga gactgcgcac
   135661 gaaccgttgc ggtcagttgc tgagtattcg gtgaaggcaa tgcccctatt ttcccggcag
   135721 taacctgcac gttttgcgct tcaatagcac tttgcacgtc tgaaggcatt agcgagtaag
   135781 acgccagttt ggccgggtca agccagatgc gcatagcgta ttccgcgcca aagacttgca
   135841 gactccccac accttcaaca cgcgccagcg ggtcctgaac gttactaacc aaccagtcgg
   135901 cgatatccga actggaggct ttgtcagtgg tgtcatacac ggcagcaatc agcaaaaagt
   135961 tgctttgtga tttctccacc gtaacacccg tttgctgcac ttcgctgggt aggcgcgact
   136021 ccgcctgctg aattttattc tgcacctgaa cctgtgcagt atctggatcg gtaccttgtt
   136081 caaaggtcac attaatactg accgaaccat cagagctgct ggttgagctg aaatagagta
   136141 aattatcaag cccagtgagt tgttgctcga tgacctgagt cacgctgttc tccagcgttt
   136201 cggcagaagc accagtataa gtggctgaaa ttttaatggt cggtggcgca acgtcaggat
   136261 attgcgcgac gggcaatgtg cgaatagcca gaatccccgc caacataatc aaaatggcga
   136321 taacccaggc aaagaccggg cggcgaacaa aaaagcgcga aaacatcagg cgttgcctcc
   136381 attagcctga acttcaacag ccttgaccgt ctgccctgaa gtgactttgg cagaaccttc
   136441 aacaatcagt cggtcgccgc tgtgcaggcc gtttagcacc agccatttat caccatatgt
   136501 ttctcccgtt tcgagcgttc gctgctctac tttattgtct ttattcacca ccagcgcagt
   136561 tgcattgcct ttagcatcgc gcgtgacgcc ctgttgcggc gcgagaatag cgtcttccat
   136621 cacgccttca tcgacacgag cgcgaacaaa cattcccggt aataattgct gttgtggatt
   136681 ggggaaaatc gcccgtaatg tcaccgaacc ggtagactca tctacagcga cttcggtgag
   136741 ttccagacgc cctttttcgc tgtaggttgt gccatcttcc agaataagtg agacgctcat
   136801 ggtgtcactg ttggtcgcca gtgactgttt acgcagacgt aataaatcga cactggagcg
   136861 agtgaggtcg acatacattg tatccagacc acgaatagtc gttaacgctg tatcttgcga
   136921 cgcggtcacc agtgcgccag gggttaccga cgaaatgcca atgcgccctg aaatcggtgc
   136981 ggttaccgtg gtccagtcaa gattaatgcg cgcagtttcc agtgcggctt ttttcgccgc
   137041 tacactggct ttatcttgtg cacaggtaga ctgagcatca tcagcatcct gttgtgaaac
   137101 accgttctct ttcaccagtc gggcataacg ctgcgctttc tggcaatcgg cttttaccag
   137161 tgcctgtgct tgttgtaatg ctgcccgagc ttcattccat gcagcctgat aactggccgc
   137221 atcaatctgg tagagcggct gtccagcctt gaccagatca ccttccttaa ataagcgttt
   137281 ctggataatt cccccaacct gcggacgtac ttcggcactg agcgcagcac tggtgcgtcc
   137341 ggttaattca ctgactaccg agaccggttg agatttgact gtaacaacgc cgacttctgt
   137401 ttctgacggg gatgaattgt tactttgcgt attatcacaa ccagataaga gcagcattgc
   137461 cactacagat gttgctatat acttcattat tttctccagg gtgagaaata ccctcctcat
   137521 taaaattaat gaggatatgt cagggatatt cagttataac gaacccgcat cgcgaaatga
   137581 atttcgcaat gaaataaggg ggattaatat cccccgctat tggttaattc tgttcttatt
   137641 tatttatcaa catactaaca atatcctgat atatttcctg cagatgttgt ggatcgcttt
   137701 tttgcggtgt taaacgacga taaaccgtac cttccatcat ggtagcaaac acttcaacgc
   137761 agcaacgaat gtgctcctcg ctaagatggg gatgcattct tttcatatgt gcacaaccgt
   137821 tagcgaacat tctggcgtca gcttcttcaa gcattgccat aacccgcggg ttgcgtgtgg
   137881 cttctgcagc cacttccagc atcagcgcgt cgtcgtcctc atttaaggat ttacgcaagg
   137941 caaggacttc cgggaagtga tcggtacgcg cgtcaatgtc catttgggca atacgaaagt
   138001 cgatgatacg gcgaaccatc tcttcaataa tggcgtcttt gttggcgaag taacgatata
   138061 tctgacccac gctaagctga gcttcgctgg caatttgcga catactggcg gcatgaaacc
   138121 cgcagcgacg aaaacaccga cgggcggcgt taatgatttc atcctgacgc tgtttgtggc
   138181 gtatttccag cttgctggtc atttctgtct cctggcctat cacttggtga gaatgatcgt
   138241 tctcattttt gctgattata cgtttctcca aagtaatgga aagtattttt atcttattca
   138301 tggtgcatat tctgataatg aagagattgt ggagattctg tgaagatagt tgacaatatt
   138361 aagttggggt caattcatca gaaaggctgc tacttgttgt cgccagttct gttttcatca
   138421 tcaaacttat cggagctaat acgaatagtt tcatagtcag attctataag atatttcccc
   138481 aacctacccc ttgcaggcgt caagagtaga taacgcaaaa tgctctgtac cgcagcttct
   138541 ctccggtaca gaaagcgcaa ctatagccac ccacagccag gttgattata ataaccgttt
   138601 atctgttcgt actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc
   138661 aacaataagg attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt
   138721 tgccagcaaa ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga
   138781 actggcattc acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca
   138841 attgggttct gacatcgttc tgcaatgcga tgttgccgaa gatgccagca tcgacaccat
   138901 gttcgctgaa ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt
   138961 tgcacctggc gatcagctgg atggtgacta tgttaacgcc gttactcgtg aaggcttcaa
   139021 aattgcccac gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat
   139081 gctgaatccg ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc
   139141 gaactacaac gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc
   139201 gaacgcgatg ggtccggaag gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac
   139261 tctggcggcc tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac
   139321 cccgattcgc cgtaccgtta ctattgaaga tgtgggtaac tctgcggcat tcctgtgctc
   139381 cgatctctct gccggtatct ccggtgaagt ggtccacgtt gacggcggtt tcagcattgc
   139441 tgcaatgaac gaactcgaac tgaaataatc gttctgttgg taaagatggg cggcgttctg
   139501 ccgcccgtta tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc
   139561 cttcccgcct tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa
   139621 cgccacatca ccggcaaaag ccactggtat catgaaacgc aatccagtac tacggagtat
   139681 gacgttctgc ctctggtccc ggaagccgca aaggtcagcg atccctttct actcgacgtg
   139741 atccttgaaa aagaaacgct ggcccccttc ctttcatggc tggaccctgc gcgtgttctt
   139801 gcagtggagt tgttccctga ccagcttacc gtgacccgtt cacagacctt caccgcttat
   139861 gaacgcttgt cgacggccct gacggttgct caggtttgcg gcgtccagcg gttatgtaac
   139921 tactattcgg cgcgacttac gccgctcccc gggcctgatt ccaccaggga aagtaatcat
   139981 cggttggcac aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac
   140041 aaccgatcgc gccagcatct gaatgacgtc ggtcttactg cctgggactg tgtgatcatt
   140101 aaccaaatca ttggttttat tggctttcag gcgcggacaa ttgcgacatt tcaggcttat
   140161 ctcgggcatc cggtacgctg gttacccggg ctggagatac aaaactacgc cgacgcgtca
   140221 ctgtttgctg atgaatcatt acgctggcga agcagctatg aggtggaaaa actacctgaa
   140281 gaacacacaa aaagttcaac tgcagaactt tgccaactgg ccgaaatact ctctctccac
   140341 cctatttcac tttcccttct cgaaaggttg ttaaacagca cacgggttaa tacacagccg
   140401 gataatcagc ttgcggcgtt gttatgcgcg cggataaatg gcagtcctgc ttgttttgcc
   140461 gcctgtatgg attcatcaaa tgaatataaa aaaatcagcc cccttctgcg caagggcgaa
   140521 aatgaaatta accaatgggc tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata
   140581 caatggctga cccgagcacc cgatcgcttt agcgccgccc agttcagccc tttactcgaa
   140641 cacgaaaaat catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg
   140701 ataaatcgct taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt
   140761 cgcgtaaaac tgtcagccgc tctaatggcc accaaaatag acaattatgt ttcaggacaa
   140821 cccgctgcta gcgcagctta aacagcaact gcattcccag acgccacgcg ctgaaggggt
   140881 ggtaaaagcc acagaaaaag gttttggctt cctggaagtc gacgcgcaaa aaagttattt
   140941 cattccgccg ccgcagatga aaaaagtcat gcatggcgac cgaattatcg cggtgatcca
   141001 cagtgaaaaa gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt ttctgactcg
   141061 tttcgtgggt aaagttcagg gcaaaaatga ccgtctggcc atcgttcctg atcatccact
   141121 cttaaaagac gccattcctt gccgcgcagc ccgtggtctg aaccacgagt ttaaagaagg
   141181 cgactgggca gttgccgaaa tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc
   141241 tgaactgaca caatacatca cttttggtga cgaccacttt gtaccgtggt gggttaccct
   141301 tgcgcgccat aatctggaaa aagaagcacc agacggcgtc gctaccgaaa tgctcgatga
   141361 aggtctggtt cgtaaagatc tgaccgcgct ggattttgtc accatcgaca gtgccagcac
   141421 agaagatatg gatgacgccc ttttcgctaa ggcgttgccg gatgacaaac ttcagctgat
   141481 tgtggcgatt gccgatccaa ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc
   141541 gaaaattcgc gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga
   141601 gctttctgac gatctctgct cactgcgcgc caatgaagtc cgcccggtac tggcatgccg
   141661 catgacgctc tccgctgatg gcaccattga agataatatc gaattctttg ccgccaccat
   141721 cgaatccaaa gcgaagctgg tgtatgacca ggtttctgac tggctggaga ataccggtga
   141781 ctggcagcct gaaagtgaag caattgccga acaagtccgt ttgctagcgc aaatttgcca
   141841 acgccgcggc gagtggcgtc ataaccacgc actggtgttt aaagatcgcc cggattaccg
   141901 ctttattctc ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc
   141961 caaccgtatc gtcgaagaag cgatgattgc cgctaacatt tgtgcagctc gcgtactgcg
   142021 cgataagctc ggttttggta tctataacgt gcatatgggc tttgatccgg cgaatgccga
   142081 cgcgctggca gcgttgctga aaacgcacgg tctgcatgtc gatgccgaag aagtgctcac
   142141 gctggacggt ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga
   142201 cagccgcatt cgtcgcttcc agtcatttgc tgaaattagc actgaacccg gtcctcactt
   142261 tggcctcggt ctggaagcat acgccacctg gacttcgccg atccgtaaat atggcgacat
   142321 gatcaaccac cgtctgctga aagcggttat caaaggcgaa actgcgacgc gtccacagga
   142381 tgaaatcact gtccaaatgg ccgagcgtcg ccgtctcaat cggatggcag aacgtgatgt
   142441 tggtgactgg ttatacgcac gcttcctgaa agacaaagcc gggaccgaca cccgtttcgc
   142501 agcggaaatt gtcgatatca gccgtggcgg catgcgtgtt cgtttggttg ataacggcgc
   142561 tatcgccttt attcctgcac ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca
   142621 ggaaaacggc accgtacaaa ttaaaggtga aacggtttat aaagtaactg acgttattga
   142681 cgtcaccatt gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta
   142741 atctcctttc acggcccatt ccttatgaat gggccgttta tttccccgct ctaccttcat
   142801 catatcccgg cagtttttta actgactttc gtttgaaaac tggcgttttt ccaaaactag
   142861 attacaaata aatatataaa aaaaacaatg tgtttagccg ccgttaattt catgtaagtc
   142921 atgttgttcc ttttatttaa ccgcggactc cgctgttaac cggaggatat gcatcatgaa
   142981 aaccgttagg gagtccacaa cgttgtacaa ctttctcgga tcgcacaatc catactggcg
   143041 gttgacggaa agcagcgatg ttttgcgctt ttctaccacc gaaaccacag aacctgaacg
   143101 tacgttgcag ttatctgccg aacaggctgc tcgcatcagg gaaatgacgg tcatcacctc
   143161 cagcctgatg atgagtctga ccgtcgatga gagcgatctt tctgtgcatc tggtaggacg
   143221 aaaaatcaat aaacgggaat gggctggcaa cgcgtctgca tggcatgaca caccagcagt
   143281 tgctcgtgat ttatcacacg gactttcctt tgctgagcag gtagtttctg aagcacattc
   143341 cgcaatagtg attctcgaca gccgggggaa tatccaacgc ttcaatcggt tatgtgaaga
   143401 ttacacaggg ttgaacgaac acgacgtcat tggccaaagc gtgtttaaac tgtttatgag
   143461 ccgtcgtgaa gctgcggcat ccaggcgcaa taaccgtgta ttttttcgaa gcggcaatgc
   143521 atatgaagtc gaactgtgga taccaacacg taaaggccag cggctgtttc tgtttcgcaa
   143581 taaatttgtc cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggcaccga
   143641 cattaccgaa gagcgccgcg ctcaggaacg actgcgtatt ctggcaaata ccgacagtat
   143701 caccggactg ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc
   143761 agataacaat aaagttgggg tcgtgtatct tgatttggat aattttaaaa aggtcaacga
   143821 cgcctatggg catttatttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag
   143881 ctgtctcgaa catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc
   143941 atccaacacc tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg
   144001 gctccccttt cgcattggtt taattgaagt ttataccagc tgttcagtag gtattgcact
   144061 ctctcccgaa catggttcgg acagcgcggc tattattcgt cacgccgaca cagcaatgta
   144121 cacagcaaag gaaggcggac gaggacaatt ttgcgttttt accccagaaa tgaatcaacg
   144181 ggtatttgaa tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt
   144241 ggttattcac tatcaaccca aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact
   144301 agtacgttgg cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc
   144361 agaagagtca gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca
   144421 ggtggcgaag tggcgggata aaggcattaa cctgcgagtg gcggtaaaca tttctgcacg
   144481 tcagctcgcc gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt
   144541 tgaatactat tgatgttgaa ttgacagaga gttgtctgat tgagaatgat gaactggcac
   144601 tgtctgttat tcaacaattt agccaactag gtgcgcaagt gcatctggat gattttggta
   144661 ccgcctactc ttcactttcg caactggcgc gctttccgat cgatgccatc aaacttgacc
   144721 aggtttttgt tcgagatatt cacaaacaac ctgtctcgca gtcactggtt cgggcaatcg
   144781 tcgctgtggc ccaggcattg aatcttcagg tgatcgccga aggtgtagag agtgcaaagg
   144841 aagatgcttt tttaaccaag aatgggatca atgagcggca aggatttttg tttgccaaac
   144901 cgatgcccgc cgtcgccttc gaacgctggt ataaacgcta tctgaagcgc gcataagtag
   144961 atgaatatat atacttagcg ctatattccg tcattatata tcgtattccc ggatgataat
   145021 tagccgttgc tgttaaacac ttctctttta gcggcaattt ttgcattatt cattttcccc
   145081 cttttgctat aaggatgtcc ttgatgtctg aatttgatgc ccagcgcgtt gccgaacgta
   145141 ttgatattgt gttagatatc cttgtggcag acgattacca ctctgcgatc cataatctcg
   145201 agatacttaa agcagaactc ttgcgccagg ttgctgaatc tacgccagac atcccgaagg
   145261 caccgtggga gatttaacaa tcgtgccttc aggttttcct atttgaggat gcccggcgcg
   145321 tcagtatttt tttatttagt attataacgt tataagaatt acagcgatga attcccgaca
   145381 acaaactatt ctacagatgg tcattgacca gggtcaggtt agcgtaaccg atctggcaaa
   145441 agccactgga gtttctgaag ttaccattcg ccaggatctc aacaccctcg aaaaactgag
   145501 ttacctccgc cgtgcacatg gctttgcagt ttcgcttgat agtgatgacg tcgaaacccg
   145561 tatgatgagc aactatacgc tgaagcgtga actcgccgag tttgccgcgt cactggttca
   145621 accgggcgaa accatcttta tcgaaaatgg cagcagcaat gccctgctcg cccggactct
   145681 gggggagcag aagaaaaatg tcactatcat cacggtcagc agctacatcg cgcatttgct
   145741 gaaagacgcg ccttgtgaag ttattttgct cggtggcgtg taccagaaaa aaagcgaaag
   145801 tatggttggc ccgttgacac gccagtgcat ccaacaggta catttcagca aagcatttat
   145861 tggtattgat ggctggcaac ctgaaactgg attcaccggt cgcgatatga tgcgtaccga
   145921 tgtggtgaat gccgtgctgg aaaaagagtg cgaagcgata gtcctgactg acagctcgaa
   145981 atttggtgct gtacattcat actccatcgg tcccgttgag cgattcaatc gcgtgattac
   146041 cgattcgaaa atacgcgcca gcgatctgat gcatcttgag cacagtaaac tcaccgttca
   146101 cgtcgttgac atttaaccat ttcccttctc tacggatgat ttgcagtttg acaaatcatc
   146161 cgctctaaga tgattcctgg ttgataatta agactattta cctgttatta acactctcaa
   146221 gatataaaat tattatcagc gatataacag gaagtcatta tcacctgcgt gatataaccc
   146281 tgcgcgcgag cggatttcac ggaataattt caccaggctt attcttagct attatagtta
   146341 tagagagctt acttccgtga atcataaatt caggagagag tattatgttt gtaacgagca
   146401 aaaaaatgac cgcggctgtt ctggcaatta ccctggcaat gtctctgagt gcctgttcta
   146461 actggtctaa acgggaccgc aacaccgcaa tcggcgcggg tgcaggggca ttaggcggtg
   146521 cagtactgac cgatggcagt acgttgggta cattaggtgg cgcagctgtc ggtggtgtta
   146581 ttggtcacca ggtcggtaaa taatcgtaaa taaatgtcga ttatcgactc gtaattataa
   146641 tacgcttgat tctgttcagt acgaaataac tgaataatac acacagtaaa atattcgaaa
   146701 gccacggata tatccgtggc tttttatgtt aaccgcctgc gagttttacc ttcatccctt
   146761 tcgcttcaag tagtgatttt aataaatcac gcttatcgcc ctggatttca ataactccat
   146821 ctttaactgc tccgccgcag ccgcattttt tcttcagttc cgctgcaagt tttgtcagtt
   146881 cggcatcatc gagatcgata ccggtaatca ggcaaacgcc cttaccttta cgtccactgg
   146941 tctgacgctg aatacgcacc acaccatcgc ctttagagcg tactggggcc gctttgggct
   147001 catcaatacg cccggtttct gttgagtaga ccagacggct gttggaatca ctcatcatgc
   147061 actccgctgt aaagaggcgt tgatcgcttt cagcgtctgc gctggatcta ccgattgcgt
   147121 taccgggcga ccaatcacca tataatcaac accagccgcc aacgcctgtt ctggcgtcat
   147181 aatgcggcgc tggtcaccag cttcactccc ctgcggacga atgcccggcg taaccagttt
   147241 gaactcctga ccgaatacct gtttaaagcg cacagcttcc tgagcagaac acaccacacc
   147301 atcaaggcca catttttgcg tcagtgctgc cagacgttct gcataatctg caggtgacag
   147361 tgtcacgcca agatcggcca ggtcgctggc ttccatgctg gtcaacactg tcacagcaat
   147421 caaaagcggt gcatctttgc caaacggaac cagtgcctca cgcgctgcgg tcatcatacg
   147481 cgccccacca gaggcatgaa cattcaccat ccacacgcct aagtcagctg cagcagcgac
   147541 agcgtgcgct gcagtgttgg ggatatcgtg gaatttcagg tcaagaaaga tatcaaaacc
   147601 acgctgttga agttcgcgca caaactgtgg cccaaacaat gtaaacatct ctttgccgac
   147661 cttcagacga caatcgcgtg ggtcgatctt gtcgacaaag gccagcgcgg catcacgatt
   147721 atgataatca agggcaacaa ccacaggaga attcgtaaca gcgcgggaag aagatgaagc
   147781 agttaacgtc atgaccagac cttcttgatg atgggcaccg gaagtggcgc gaaacaggta
   147841 aacggcgagc attctacccg tggaaataaa aaattaacag ttgcgatttc tcccctggca
   147901 ggctttttct gcgccgccgt tgccggagca caatgggaca taaataacta gtatgttgta
   147961 actaaagtcg gtttttttaa aaaattacag gccatcaaga ccgcgaatcg gtttaatagt
   148021 tgaccaggca cgacaagacg gacaatgcca gtagagggtg tatgcggtaa aaccacattt
   148081 ctggcagcga taacgaggct tactacgtac cttctcgcca accatgtcac gcagcaccat
   148141 caggctctct ttggcacgcc cttcttccgc ttcatttaag tggtaatcca ttaacttatg
   148201 gaacacacgc atggtcggat gacgctgaag ctggcgcgta atatagacct gtgcggcctc
   148261 actaccgtcg cgcgcttcga tgatatcagc aagcatcaat tcagcatcgg caccggtgtt
   148321 ctcttccacc gcgcgctgca ggaattctgc ccattcggca gttttaccca actgctgata
   148381 gcaggtttgc agcatttcca gcgtttcgct gaccagttct ctgtcctggg atatcacgcg
   148441 ttgcaaactt tcgacggctt tggcgtattc tcctttcgcc ataaacacgc gtcccatcat
   148501 gatggatacg cgggcgctgt ttttatctgc cgccgcccct ttttttagca aggtcatggc
   148561 acgatcgaga tcgtcgctgg ccatatgctg cagggctaac tcacagtaga aatgggcaat
   148621 ttcgacgcgc tgtttatctt tacccagctt caccaggcgt tcggcaacat caattgcttt
   148681 ctgccactca ctggtagcct ggtagatttg tagcaactgt tgcagcgcgc caatgcggaa
   148741 gtcagtttca tcggtcagct gattgaacat gtcttccgcg cggtcatata acccggcggc
   148801 catgtaatca cgccccagtt gttgaatcgc caacagacgc tgttcatagg tcagcgaggc
   148861 gctttccatt agggtctgat ggatgcgaat agcgcgatca acttcgccac gcgaacggaa
   148921 caggtttccg agcgtaaggt gggcttcaac ggtgcctgta tcctctttaa gcatatcgag
   148981 aaacaggtct accgctttat cctgttgatt actaagcagg aagttaaccc ccgctacgta
   149041 atcacgcgac aagcggttgg cttcatcttg cttgttttgt tgcgcacttc tgcggcccat
   149101 ataccagcca taggcagcgg ctacaggcaa aagcagaaac aacaactcca gcatagaaag
   149161 ttattccttc gccgctgacg agtgctgcac tacagccacg tcagtcgcgg gtgaaagctg
   149221 gttttccagt cgctttattt tacgttcagc gcgcaccagg gaaacacgaa ctcgcagcca
   149281 gaacaggcca caaatcaacc aaccgatagc aaaacccgca gcaaacaata ccgccagcaa
   149341 tgtggaaata cggtactccc cttgcgctaa cagataatta aacgtcacct gttgatcgtt
   149401 ctgcgcaccc aacgtgaccg aaatcacgaa gatcgctaac accagtaaga aaatgagtaa
   149461 atatttcaca ttacttcccg ttatgtggtt agagcgaata aattatggtc aacttaccgc
   149521 aatagccctt taaagtacca ttttcgcgcc gtctgcgaaa tggcatccac gcgcattgtt
   149581 gatgatttaa gggctaaatt cggaaaatca accagcgtta actttcttgt tctcgttgcg
   149641 ctatttcgcg attttcttcc gcaggtggtg ttaatggccc gcaaattcgt tgcgcaagcc
   149701 acgttgccac cgccaccagt gcccacgaaa tcaacgtagc taccaccaga tcgcgtggcc
   149761 aatgcatccc gagcagcagg cggcttccca tgactcccgt tgcccagacc agcaagatag
   149821 caatggttaa cgttcgccga cgcggccaca gcaaaccaac ggccagcagt gcccaactgg
   149881 cagcaaacat cgtgtgaccg gaaggaaagg caaaccccgt ctctttctgc cagtgtgaac
   149941 gcaaatattg tggaatattt ttctcttcag ccaactgttc tttcactaga tttccgcgtt
   150001 ctgctcgctt taaagtgtag aactcatcaa ccggaatatg atgtgttttt tccagccaga
   150061 taacaaaagg tcgtggttcc tggactttgt ctttgatcca ggatttaacg ccttgtccca
   150121 caaggattgc ggccgccaga atggcaaata atacaaaggc agccttaatg cgaaaacgca
   150181 gacaccagag aaaccagccg aataaaatca aatgtgtaat gacgccccag ggctgggtga
   150241 cagtttcagt aacccaaaaa gccgctttta gtagccaact ttgttctcca ggttgccaac
   150301 gccagccaga aatccatacg gctactggca taacaagcaa tagtgcagct cccactgcgg
   150361 tacgtcttgc aatcgaacgc atggcctctc ctttttgata agtcccacaa tcataactga
   150421 aaatgccagt tccaggaaaa attgacagat ttgtgccatt ccgtgaacga tcgacgcgtc
   150481 gtgattaggt gaaccccttc tcgttatggc aaaataagcc aatacagaac cagcattatc
   150541 tggagaattt catgcagctt aaacgtgtgg cagaagccaa actgccaacc ccatggggcg
   150601 atttcctgat ggtgggattt gaagaactgg caaccggaca cgatcatgtc gcgctagtct
   150661 atggcgatat ttccgggcat accccggtac ttgcgcgcgt ccattccgaa tgtctgaccg
   150721 gtgacgccct gttcagcttg cgctgcgatt gtggcttcca gctcgaagcg gcattgacgc
   150781 aaattgccga ggaaggccgt ggtattttgc tgtatcaccg tcaggaaggt cgtaacattg
   150841 gtctgctgaa taaaatccgc gcttacgcac tgcaggatca aggttacgat actgtagagg
   150901 ctaaccacca gttaggcttc gccgctgatg agcgcgactt cactctttgc gctgatatgt
   150961 tcaaactcct tggcgtcaat gaagtccgct tgttaaccaa taacccgaaa aaagtcgaaa
   151021 ttctgaccga agcggggatt aatattgttg aacgcgtacc attgattgta ggtcgtaacc
   151081 ccaataacga acattatctc gataccaaag ccgagaaaat gggccatttg ctgaacaaat
   151141 aaccctcttg cattgtgtaa ttaatttgct tgccggaagc aaaataaccg gcaagcaaat
   151201 acttgttact tcaacatatt acgaatgaca taatgcaaaa tgccgtcgtt ctggtagtaa
   151261 gtcaactccg tcgcggtgtc gatacgacaa cggcagggta cgacttcctg gctaccatcc
   151321 gcgcgcgtaa gcgtcaccgg aaccgtcgcg ccgggttgta gattttgcag atcgccaata
   151381 tcaatcttct cttccccggt tagccctaac gttttacgcg ttacgccttg cggaaattcc
   151441 agcggcagga tgcccatgcc aattaaattc gaacggtgaa ttcgttcaaa cgattcggca
   151501 atcaccacac gaataccaag cagacgcgga ccttttgccg cccagtcacg gctggagcct
   151561 gatccatact ctttcccggc aatcaccgcc agcggcgttt gctcctgctt atagcgcatc
   151621 gcagcatcat aaatagagac tacgtcgctg tcaggtaaat gccgcgtcat ccccccttca
   151681 acgccaggca ccatttcatt acggatgcga atattggcga aggtgccgcg catcatcact
   151741 tcatggttac cacgccgcga accgtaggag ttaaagtctt ttcgctcaac accccgacct
   151801 tgtaggtatc gacccgctgg gctgtcgggc ttaatactgc ccgccggaga gatatggtca
   151861 gtggtgactg aatcccccag cattgcgagg atccgcgcac cgtgaatatc ttccacaggt
   151921 gctggcgttg cctgcatttc atcaaagaaa ggcgataaac gaatataggt tgaatcctcc
   151981 tgccatccgt aggtatcgga tcgtgcgacg ttaatttctt tccactctgc agtgccttca
   152041 aagacttctg cgtactcttt gcggaacatt tctgtggaga cttgttctac cgcacgggca
   152101 atttcttgtg ccgatggcca gatatctttc agatatattg gatcgccttt acgatcatga
   152161 ccaataggct cagaagccag gttgatattc atatttcccg ccagcgcata ggcaaccacc
   152221 agcggcggcg aggccagcca gttagtttta accagcggat ggatacggcc ttcaaagtta
   152281 cggttgccgg acagcactgc accgacggtt aaatcgcctt ttttgattgc cgtttcgata
   152341 ggatcgggca gcggcccaga gttaccaata caggtggtac aaccgtatcc cacaaggtta
   152401 aaccccagtt cgtcgagata cggtgtcagt ttcgcttttg ccagatagtc agaaacgact
   152461 ttcgaacccg gtgccagcga cgctttgacc cacggttgcc gcttgaggcc aagagttacg
   152521 gcttttttcg ccagcaagcc tgcggccatc agcacacttg ggttagaagt gttggtgcac
   152581 gaggttatcg cagcaatgac cacagcgcca tcaggtaact gatactgatg tccgttcata
   152641 acataatcga ccggctggcg atctttatgc gtggcattca cttccagttc gttactggcg
   152701 gcaaatgctt ttggtacatc gggcagtgca acgcgatcct gtgggcgttt aggccctgcc
   152761 aggctcgctt caacgtcatt catatccaga tctaacgtac tggtaaaaat cggttcatcg
   152821 cccgggttac gccacatgcc ctgcgctttg gcatattttt cgaccaactc gacctgatct
   152881 tcgctgcgcc cgcttaaacg catgtaatcg agggttacag catcgattgg gaagaagcca
   152941 caggtggcac catattctgg cgacatattg gcaatggtgg cgcgatccgc caacggtagt
   153001 gaatccagac catcaccata aaattcgacg aatttcccca ccacgccatg tttgcgcagc
   153061 atttgggtaa cagtgagaac caggtctgtg gcggtaatac cttcacgtaa ttttccggta
   153121 agtttaaagc ccactacgtc cgggataagc atggaaaccg gctggcctaa cattgcggct
   153181 tctgcttcga tgccacccac gccccacccc agcacgccaa ggccgttgat catagtggtg
   153241 tgcgagtcag taccaacgag tgtatccgga taagcaatcc attcacagtc ctgcaattcg
   153301 ctccacactg ctttgccgag atattcgagg ttaacctgat ggcaaatgcc tgtgcctggc
   153361 ggcacgacgc taaaccgact gaacgcttgc tttccccatt tcaggaacac ataacgttcg
   153421 tggttgcgct ccatttccag gcgtacgttt tcttcaaatg cctcatcatc accaaaacga
   153481 tcgacggtca ccgagtggtc aatgaccagg tcgaccggtg agagcgggtt aacctttgca
   153541 gtatcgccac cgaggcgttt aaccgcttcg cgcattgccg ccagatcaac aacggcaggt
   153601 acgccggtaa agtcctgcat cagcaccctt gccgggcggt aggcaatttc acggtcagca
   153661 tgggcatttt tcagccatcc cgccagcgcg tggatatcct cttcggtaac cgagttacca
   153721 tcctgccagc gcagcagatt ttcgagcaaa actttgagtg acttgggtag acgggtgata
   153781 tcgcccagtg atttggcagc aagcggcagg ctgtagtagt ggtaagtttt atctttggcc
   153841 tgcaacgcgt ccttactggc ttctcgtagg gttgacgaca tagctcctcc ttaatgacag
   153901 ggttgcgaag ccctgattct cttcagggtg aatattaaag ataacacaaa catcgcgtaa
   153961 cgatttgata acaacccaaa ttgataaaag aggagaaacc ttgtggcaaa gcaaatgaca
   154021 accccgccgc agcggggtca aggatcagtg agtaatgatc cagaggagtt cggcccagaa
   154081 gagcatcgac agagaaaatg cacccatcca ggaccaatat ttcatatttg tgttgatcat
   154141 aggctacacc cttaacggtt attactttgt gttgagataa ctcaacctgt tattgctgcg
   154201 gtgtattaaa agccctttaa ttcattaaaa atgggtaata ataatgatga taattattgt
   154261 ttcggctgat catcttcagc aaacagttct ataaaggctt gctgctgcga tgttagttgc
   154321 cagtcgtctg gaataacgac ggaacattcc cgtttcgttt cgggttcatt gtcactatgc
   154381 tggcataatg gtgacgactc cagttgctcc tgaccaacca tagttatccc cacactttcc
   154441 aaatcagcag tgcgacaacc acccagaaaa gtgcagaacc aagaaaaacc gccagccagg
   154501 ctttacgttt taattcagga tcgcggcggg gttcctgatt tccagaaggc attgctaacc
   154561 tcatataatc gccatcactt atcagcaaga cgttgaacga ttggtgtaat taatcatacg
   154621 atgagacaaa tcctaaagaa actttagtca aaaatccagc gaatttacga atcgtctcca
   154681 gtgaataatt caatcttttg acctgaaata gatatttgaa aactggaatt tgcgaggcgg
   154741 gtaattagac actactttac aggtaattta ccctataaat atgagggata aaaatattgt
   154801 gcacggtata caaataagta acaaaagaga atatattccg gcaccttcgc tacataaaag
   154861 gtgccgaaaa taacgcaaga aattattttt ccggcagttt tatatcttta aacatgacct
   154921 caatttcttc attggagcgc aatgcgacag ccgcatcaac gacatcacgc gttaaatgcg
   154981 gtgcaaaacg ctgaatgaaa tcatacatat aactgcgcaa aaaagtacta cggcgaaaac
   155041 caattttggt tgtactgtgg ctgaagatat cgtgagcatc aacacgcaca aggtccggat
   155101 cggcgaccgg atccaccgcc atgctggcaa tgacccctac ccccagccct aaccggacgt
   155161 aagttttaat gacgtcagca tccgttgccg tgaaaacgat acgcggcgtt aaccctgcgc
   155221 gattaaaggc agtatccagt tctgaacgtc cagtaaagcc gaaggtatat gtcaccaacg
   155281 gatattgcgc cagttcttca atggtaatgg cttttttgcc tgccagcggg tgatccggag
   155341 tgactacaat agcccgattc cagtggtagc acggtaacat cactaaatct tcatacagat
   155401 gcagcgcttc tgtggcgata gcgaaatctg cattaccttt agagacggca tcagcaattt
   155461 gtgtcggcga accctggtgc atatgcaaag aaacgcgggg ataacgctca ataaagcctt
   155521 tgatgacgtt tggtaacgcg tagcgtgcct gggtatgcgt ggtggcgata tacagcgagc
   155581 ctttatccgg ccaggtgtgc tcaccggcaa ccgattttat ggcatcaact ttcgacagga
   155641 cttcgcgagc gatacgaatt atttcttgtc ctgctggcgt tacctgcgtc aggtgcttgc
   155701 cgcttcggga aaaaatttga atgcctagct cgtcttccag cattctgact tgtttactga
   155761 tcccgggttg tgatgtgtaa agtccttccg ctgttgatga gacattcagg ttatgattga
   155821 ccacctcaac aatatagcga agttgttgta atttcatgtt aaaccatcca cttagacgcc
   155881 atcagaacat cgttttttcg tcacacctgg cgggtgacta atacgattta ataataaaaa
   155941 aggtgctaac tataaccact atatcattta tagccttagt gtataggtgt gaagaaaaaa
   156001 taatatgcaa taaaaaaggg ccgctttcgc gaccctttgt ttataaaaac ctgacagaat
   156061 taaaggttat tttttccctt caacccattt gccatcaaca taaaatgctg accagccagt
   156121 cgcctttccg tctttttccg aagagacata ttgctgtttg gttttacggc taaagcgaac
   156181 catagtctta ttaccttccg gatcctgctg cggcgcatcg gccagataac gcagtttttc
   156241 cggcagacga tcgcggaagc gatacagctc ttccaccagc ggcgcacgcg tttcacgcga
   156301 tttcgggaag gtattggccg ccaggaacac accggcagca ccgtcacgca gcacgaaata
   156361 ggcatctgat ttttcgcacg gcagctccgg taatggtacc ggatcttctt tcggtggagc
   156421 cacttcgccg ttacgtaaaa tcttacgcgt gtttttacac tcttcgttgg tgcaggccat
   156481 atatttaccg aaacgcccca ttttcaggtg catttcagaa ccacattttt cacactcaac
   156541 gatcgggccg tcataacctt taatgcggaa ttcgccctct tcgatctcgt aaccgtcgca
   156601 ggttgggtta ttaccacaga catgcaactt acgtttcgga tcgatgagat agctgtccat
   156661 cgccgtgccg catttggggc aacgacgttt tgcgcgcagc gcgttggttt cagcgtcttc
   156721 gccttccagc acgttcagca cttcgttttc cggcaccagg ttaatggttg ttttgcaacg
   156781 ctctttcggc ggcagcgcat agccagaaca gccaaggaat accccggtgc tcgctgtgcg
   156841 aatccccatt ttgcgaccac aggtcgggca gtcgatgctg gtcagaacca tctggttcgg
   156901 acgcataccc ccctcttccg gatctttttc agctttatct aactgctgag tgaaatccga
   156961 gaagaagtga tccagtacag ctttccactc tgcttcgtga tttgccacct ggtcaaggct
   157021 gttttccatc tgcgcggtga agtcgtagtt cattaactcg cggaaattct cttccaggcg
   157081 atcggtgacg atttcgccca ttttttccgc atagaaacga cgattttcta ctcgcacata
   157141 gccacgatcc tgaatggtcg aaatgatcga cgcataggta gacggacgac cgataccacg
   157201 tttttccagt tctttaacca gcgatgcttc actgaaacgg gctggcggct tggtaaagtg
   157261 ctgggctggt gtcagttcaa cgagcgtcag agcatcgcct ttatcgactg caggtaagat
   157321 acgatcttca tcgcctttac gcagtgcagg catcactttc gtccagccat caaagcgcaa
   157381 aatacgaccg cgtgctttca ggcggaaatc gcccgcacca acggtcagcg tcgtggagtc
   157441 atatttcgct ggggtcatct ggcaggcaac gaactgacgc cagattaact ggtacagttt
   157501 ctgcgcatct gcttccatat ccttcagcga ttccgccatc acattgacgt cagaaggacg
   157561 aatcgcttcg tgcgcttcct gtgagttttc tttgctggcg tactgattcg gactttccgg
   157621 cagatatttc ttaccaaaat tatcgctgat ataaccgcga accatattta ccgcgtcctg
   157681 actcaggtta gtggagtcgg tacgcatgta agtgatatag cctgcttcat acaaacgctg
   157741 cgccatcatc atggtttttt tcacgccaaa tccaagacgg gtgctggcag cttgttgcag
   157801 cgtagaggta ataaaaggag cgccaggttt actggttgtc ggtttgtctt cacgttccag
   157861 cacgctgtag cgcgcttttt ccagcagact taccgcagcc tgagtttgtt ctttgttgac
   157921 cggacggaac ggtttgtcgt tctgatgagt cacctgcaac gccaacgctt caccagatgg
   157981 cgtggtcgtg ctggcatcga cttcccagaa ctcttccggc acgaacgctt taatttcacg
   158041 ctcacgctcg accaccaggc ggactgccac cgactgcaca cgaccggcag acagaccacg
   158101 agcgatcttt ttccatagca gcggcgaaac catatacccc accacgcggt ccataaagcg
   158161 acgcgcctgc tgggcattaa cacgatcaat attcagctca cccggtttgt taaatgcctg
   158221 gcggatcgcg tttttagtaa tttcgttaaa caccactcgg ctatagcgcg catcatcacc
   158281 accaatcact tcccgcaggt gccatgcaat ggcttcccct tcgcggtcaa ggtcggttgc
   158341 gagatagatg tggtcggctt tttcagccag ttgtttcagt tcagagacga ccttctcttt
   158401 accaggcaac acttcatagt gcgcctccca attgtgccac gggtcaaccc ccatacggtt
   158461 gacgagagcg ccacgttcat cctttttagg ctttttagcc gtcttggtgg aggtagagtc
   158521 ggcactcttt ttggcagctg agccactggt cggcaaatcg cggatgtgac cgacgctgga
   158581 tttcaccacg tagtcactac ccagatactt gttgatcgtt ttggcttttg ccggggactc
   158641 aacgatgaca agagctttac ccatattcac cttacctaat ttgattcttc caggaatgcg
   158701 tcgcacgttg ataaaccttc cactggcaac gagttactga tatggactcg ctctctgcgg
   158761 atatcaaccc cttgtcgtga ctctgttcac gaaaattcag gtgactgagt taacaggtct
   158821 tggcctacag gcgctatagc acgacgaaat tttggtcgaa tgtcaagcaa atctattgcc
   158881 agattgacga aagcgtcaca ctgtacctga taaaatccga tacgcaactt tattagcatg
   158941 caaaatccaa agttgccagt aaggcgctct tgccaccagc gtggtatatt tgtctgcaag
   159001 ccatgcctgg cgtagactaa tgtccttgtt taaggagtaa ataatgaaca aagagacaca
   159061 acccatcgac cgcgaaacgc tgttgaaaga ggccaacaaa atcattcgtg agcatgagga
   159121 tacgctcgca gggattgaag ccaccggcgt cacacagcgt aatggcgtgc tggtatttac
   159181 cggcgactac tttttagatg aacaaggatt gccaacagcc aaaagcaccg cggtgtttaa
   159241 tatgttcaaa cacctggccc atgtgctttc agaaaaatat catctggttg attaaagcaa
   159301 aacgcgaggt cttaccctcg cgtttgtctt ttacatcaat ggcttttgcc cacgctgcca
   159361 ccagcgtagc aataatcgat cggcgctctc tgccgcgctg ccggtgaatc ggtcaatgag
   159421 tcgtttacgc tgcatatagc gtacattgac cacttctcgg ccttccatca ggctaagaat
   159481 aacttcatca ctggtgttga tttcatcaac caggcccttc tctaccgcct gttgtccgta
   159541 ccagtgttca cccgttgcca cctgttcaat atccagagac ggacgcatac gcttcacaaa
   159601 atctttaaat aactgatgcg tttcgttcag ctcttcgcgg aatttctccc gcccttcttc
   159661 ggtattttca cccagcaagg tcagcgtacg cttatactgc ccggcggtgt gcagttcgat
   159721 atcaatatct ttgcttttca ggaagcggtt aaagttgggc atttgcgcca ccaccccaat
   159781 ggaacccaca atagcaaacg gtgcggaaac aattttgtcc gccacacagg ccatcatgta
   159841 accaccgctg gcagcaactt tgtctaccgt aacagttaat ggaatgtttt tatcacgcag
   159901 acgctgcagc tgcgaagccg ccaacccgta accatgcacc atgccaccag ggctttccag
   159961 acggagcaca acctgatcct gcggtttgaa tgctgcaagt accgccgtta tctcttcacg
   160021 tagcgagttc acttcatggg cgtccatgct gcctttaaag tccagcaccc agacgcgggg
   160081 tttactgtca gttgacacct cgcccagttt ggctttcgct tttgctgctt tcgcttcttg
   160141 cttgtgcttt ttcttctgcg ctttgtgcca ctgtttttgc tgatgtgagt ccatcagcgc
   160201 cgcggccagt tcttctttca tctccttata ctgttcgctg agattgttga cccgtaactc
   160261 gccacgctgg cgtttattac gttgagcaac attgacaata atggcggcaa tcgccgcaat
   160321 cgctagcaca acggtaacga ttttcgccaa aaacaaacca tattcagaca acaattccac
   160381 gagtccccac cttggttaaa caacgctttg gctcacagtt tacaacaggc atgttaagcc
   160441 gtctcgcaga gatgttattg cagcgaaagt atgacaaagt atccattttg cggtgttatc
   160501 gcccgaggct gattgaaatg cccttctgtt tcaggcataa agccccaaag tcataaagta
   160561 cactggcagc gcgacagaca acgtcgttga ggagtagcca tgcattacca gccaaaacaa
   160621 gatttactca atgatcgcat tatcctggtg acgggagcca gtgatggtat tggtcgtgaa
   160681 gccgcgatga cgtatgcacg ctatggtgcg acagtgattc tgttgggccg taatgaagaa
   160741 aaattacgtc aggtagccag ccacataaac gaagaaactg ggcgtcagcc acagtggttt
   160801 attctcgatt tgctgacctg caggtccgaa gattgccaac aactggcaca gcgcattgcc
   160861 gttaattatc cgcgtctgga tggtgttttg cataatgccg gattactcgg cgatgtttgt
   160921 ccaatgagcg aacaaaatcc acaggtctgg caggacgtca tacaggtcaa cgttaatgcc
   160981 acctttatgc tcacccaggc actacttcct ttattactca aatcggacgc cggttcactg
   161041 gtctttactt catcaagcgt tggacgtcag ggacgagcca actggggtgc atatgcagcg
   161101 tcgaaatttg ccaccgaagg gatgatgcag gtactggccg atgaatatca gcagcgcctg
   161161 cgtgtcaact gcattaaccc aggcggtacg cgcaccgcaa tgcgtgccag cgccttcccg
   161221 accgaagatc cacagaaact taaaacaccc gctgatatca tgccgctcta cctctggctg
   161281 atgggcgatg acagccgccg taaaaccggc atgacctttg acgcccaacc gggccgtaaa
   161341 ccaggaattt cccaatgagt gatgaacgct accaacagcg tcagcagcga gtgaaagaaa
   161401 aagtagatgc tcgtatggcc caggcccagg atgaacgcgg tattatcatc gtctttaccg
   161461 gcaatggaaa aggcaaaacc accgcggcat ttggtacggc aacacgcgca gttggtcacg
   161521 gaaaaaaagt gggcgtcgtg cagtttatta aaggcacctg gcctaatggc gaacgcaatc
   161581 tgctggagcc acatggcgtt gagtttcagg tgatggcaac gggctttacc tgggatacac
   161641 aaaaccgcga gtctgatacc gccgcctgcc gcgaagtctg gcaacatgca aagcggatgc
   161701 ttgctgattc ctcactggat ttggttttgc ttgatgagct gacttatatg gtggcgtatg
   161761 actatttgcc actggaagaa gtgttgcagg cgttaaatga acgtccacat cagcagacgg
   161821 tgattatcac gggtcggggc tgtcatcggg atatactcga actggcggat acggtaagtg
   161881 agttacgccc cgtgaaacat gcgtttgatg ctggcgtaaa agcgcagata gggattgatt
   161941 actaatctgt aaaaatgccg gatatgacgt ccggcatacg ttgattaacc gttattacgc
   162001 ccaccagaac gacgtccgcc gctcacctga ctgtgacgtt tcaccgcacg acgaatctga
   162061 ttcgccttca tgcgacgacg gtctttttct acagcgactt tagagctggt ttctggcggc
   162121 aactccacca gttcgcgcag atagttagtc tgggcaagat ccagctccgt ccagccgccg
   162181 cgtggcagac ctttcggcag tgggatatca ccgtaacgaa cacggatcag gcggcttacc
   162241 tgcacgccaa ccgcttccca cagacgacga acttcacggt tacggccttc ggtcagagtc
   162301 acgttgtacc actggttgat cccttcgccg ccgctgaact tgatggtttt aaaggctgcc
   162361 ggaccatctt ccagctgcac accacggctt aaatcacgca gtttcgcgtc gtcaacctga
   162421 ccaaatacac gcacggcata ttcacgttca acttcacggc ttggatgcat taaacggtta
   162481 gccagttcac cgtcggtggt gaacagcaac agaccacagg tgttaacgtc cagacgacct
   162541 acggcaatcc agcgtgcacc gcgcagcttt ggcagacggt caaacaccgt tggacgccct
   162601 tccggatcgt tacgggtaca caactcacct tccggtttat aataggccag aacgcgacaa
   162661 atttgttcag cggactcacg taccgaaatc aggtgaccat cgatacggat tttcaatcca
   162721 ggggtaactt caacgcgatc gccgagttta gcaattttgc catcaacact cacacgaccg
   162781 gcttcaataa tagattcgat ttcacggcga gaaccgtggc cggcacgcgc cagcactttc
   162841 tgtagctttt cgctcataga gcttcctttt ggtgtcgcct tcccaggcgt ctgatatgct
   162901 tttgtgcaac agtgagtcag aacttacatc tgactcactg atagtaaagt ctgtttgaac
   162961 tcttcaggcc ttaagtaaac gactcgacgt tttaactggc ctggctttga ggcgcaacat
   163021 actacactat ctttatggtt aaagcttgtt tcgattaaca tcagcttaat taccagccac
   163081 caccgcctcc gccacccgca gcaccaccgg aataacttcg ccccaataaa ctggtattgg
   163141 cccccagact caggtgcatc tgtaatgaga acaaaacgcc actacgggca atttccgggg
   163201 cgcgaacagc cgctgtgagt gatggggtga tacgtgctac ccattctctt ccaaggtttg
   163261 ctgagacagc ccaacctagc aacgcgtaat ccagtttccg tgtttcccct ttccgccgac
   163321 gcccactgtg tgttgctgcc tctttacgcg cgagatagtt aacgatagct tcaccgtggg
   163381 cataacgttg ctgcccagtt tgggtaaact ttggcagaaa aatatacccc atgccagtga
   163441 gataaccggt caggaaaaca gcgctaaaat aacccgctgg catataccag taaaagaata
   163501 catctccgac attgatatag agagaataaa aggccacgcc tgaacaaatc agcggtaaaa
   163561 acaaagtgat gattacacga ttaacgcaag gcttgtcatc atcgcgggca aacaaaagct
   163621 caagggaaca aaataaaaaa ggaagaagcc agcagatcat aataaacata tcgcccacca
   163681 gactcattcc tgcccagcct acaggactca ggttaccgta aagaatgacc gccaatattg
   163741 ccagtccacc ccattgcaaa aaagtatcag gccggtaaaa cgaagacttt ttacgcggca
   163801 gataaaattt ctgcatccgt aaaacatcta ccgacgtaaa ttgcggcctg cgcttccaga
   163861 gatataacca tccacaaacc aggaatagac acggaaacca aattaacaac gatgacgtgg
   163921 agggtaaaag aagatggctg aaaatattcg tcgtttgtgg agccggggca ttgctaagta
   163981 acgagtgagg ccaacggtag agtaccgtga aatcttcacg ataaaacgga tcgctggatt
   164041 caattcttcc gtcctcaagg atgcggccat ttcgatatgt gtcgccctct tctccagtaa
   164101 agagatcgat ttcgctaaat ggattacccg cgatatcagg gaacttgagt gaaaatcggg
   164161 tcttatagat ttcaaacggc cagtggttac cagtcacgtt ccagattaac agatctgaat
   164221 ctccctcacg caggaaagca tttttaacct gataatgaat ttgataatga tagtcgcctt
   164281 gaggcaagaa acgttgttta tcgcccacga caatactcat taacccgata tgccagtcaa
   164341 gatgccaggg ttcaggaata ccatcacggg tgacgccaac aatatgatag ttcagcagtg
   164401 cagcgtcccc atcaggctgc atccatagtc gtggtaaatc acgataaaat ccgtgcttaa
   164461 tctgctgatt tcgcgcctga aaatgaatat tttctttgat gtcgagcacc ccatccggtt
   164521 gcagagtggc ttcaacatca aagagcgaga tcccttcttc ataagaaggc atctccgtgt
   164581 ttttcgcata acttaacgaa gagaaaaaaa gcccaactat caacagcaaa atgcagcgaa
   164641 acttccctgc catagtcttc catccgtgga acgcaatttg ttataagaaa ggcttcacat
   164701 cacctacgcc ttcacgcacc acgaccggcg tatcatcggt aagatcaata accgttgttg
   164761 gtttctgccc gagatagccg ccgtgaataa tcaaatcgac ctgtttttcc agacggtctt
   164821 tgatttcttc gggatcggat tcggtaaatt cgctgcctgg cagcattagc gaagtggaaa
   164881 gcatcggttc gcccagtgct tcaagcaacg cctgcgcaat cgggttagaa ggcacacgca
   164941 taccgatggt tttgcgtttt tcctgcaaca gacggcgtgg cacctccttc gtccccttca
   165001 ggatgaaggt atagttgccc ggcgtgttgt ttttcattaa acgaaacgca acgttatcga
   165061 caaatgaata ggtcgagagt tcagaaagat cgcgacacat cagggtaaag ttgtgaccgt
   165121 ccggcagctg gcgaatacga caaatacgct ccatggcgtt tttatcttca attttacagc
   165181 cgagcgcata gccggaatca gttggataaa caatcacccc gcctttacgc acgatctcca
   165241 ccgcctggtt gattagacgt tgctgcgggt tatcaggatg aatataaaaa aactggctca
   165301 taattccctc tctgtggtgt tctgcggctg ttcccatagc tgccagacgc cttcaacgcc
   165361 tgcgggcagc cagagtttac gacccagttc gatccacggg catggctgat gaaaatcaga
   165421 tccttgtgat gcccataaat ggtgctggcg ggcaagcgtc gccagctggg tacgttcatt
   165481 gggcgattgc tggcactgcg cgacttccat cgcgtcgccg tcgtgttcgg caaaatgcgc
   165541 aaccaatctt ttcagccatt tagcactaag attgtaccgc ccaggatgag ctagcaccgc
   165601 cttaccgcca gaatgatgaa tgacatcaat agcttgttct attgtacacc actgtggcgg
   165661 aacgtatccg gttttcccgc gcgccagata ctttttaaag acatccgcca ttgaactggc
   165721 tttgccgcac tcaacaagga aacgcgcaaa atgaccgcgc gtcactgcgc cgccctgcgc
   165781 cagtcgttgt gcgccttcca gcgccccagg tatatgcgct ttttcaagtc tttcggcaat
   165841 ttgctgcgcc cgctgattgc gccgttctgt ctgctgcgcg aggaactcac acattagcgg
   165901 atgagtaata tcaatattca gcccgacaat atgaatttca tgattttccc agaccgtgga
   165961 aatttccacg ccgggaataa gattcagcgc cagtcccgaa cgtgaaattt cctctcttgc
   166021 tggcgcgatt gcagctgtgg tgtcatggtc ggtgatcgcc agggtgccga cgcgcatctc
   166081 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
   166141 gtgcaggtcg taaatcactg cgtaattcgt gtcgctcaag gcgcactccc gttctggata
   166201 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
   166261 atcaatcatc gaactagtta actagtacgc aagttcacgt aaaaagggta tcgacaatga
   166321 aagcaatttt cgtactgaaa ggttggtggc gcacttcctg aaacgggcag tgtattcacc
   166381 atgcgtaaag caatcagata cccagcccgc ctaatgagcg ggcttttttt tgaacaaaat
   166441 tagagaataa caatgcaaac acaaaaaccg actctcgaac tgctaacctg cgaaggcgct
   166501 tatcgcgaca atcccaccgc gctttttcac caattgtgtg gggatcgtcc ggcaacgctg
   166561 ctgctggaat ccgcagatat cgacagcaaa gatgatttaa aaagcctgct gctggtagac
   166621 agtgcgctgc gcattacagc tttaggtgac actgtcacaa tccagtccct ttccggcaac
   166681 ggcgaagccc tgctgacact actggataac gccctgcctg cgggtgtgga aaatgaacaa
   166741 ttaccaaact gccgtgtgct gcgcttcccc cctgttggtc cactgctgga tgaagacgct
   166801 cgcttatgct ccctttcggt ttttgacgct ttccgcttat tgcagaatct gttgaatgta
   166861 ccgaaggaag aacgagaagc catgttcttc ggcggcctgt tctcttatga ccttgtggcg
   166921 gggtttgaag atttaccgca actgtcagcg gaaaataact gccctgattt ctgtttttat
   166981 ctcgctgaaa ctctgatggt gattgaccat cagaaaaaaa gcacccgcat tcaggccagc
   167041 ctgtttgctc cgaatgaaga agaaaaacaa cgtctcactg ctcgcctgaa cgatcttcgc
   167101 cagcaactga ccgaaaccgc gccaccgctg ccggtggttt ccgtgccgca tatgcgttgt
   167161 gaatgtaacc agagcgatga agagttcggt ggcgtagtgc gtttgttgca aaaagcgatt
   167221 cgcgccggag aaattttcca ggtggtgcca tctcgccgtt tctctctgcc ctgcccgtca
   167281 ccgctggcgg cctattacgt gctgaaaaag agtaatccca gcccgtacat gttttttatg
   167341 caggataatg atttcaccct gtttggcgcg tcgccggaaa gttcgctcaa gtatgacgcc
   167401 accagccgcc agattgagat ctacccgatt gccggaacac gtccacgcgg tcgtcgcgcc
   167461 gatggctcgc tcgacaggga ccttgaaagc cgtattgaac tggaaatgcg taccgatcat
   167521 aaagagcttt ctgaacatct gatgttggtg gatctcgccc gtaatgacct ggcacgcatt
   167581 tgcacccccg gcagccgcta cgtcgccgac ctcaccaaag ttgaccgtta ctcttacgtg
   167641 atgcacctgg tctcccgcgt ggtcggtgag ctgcgccacg atctcgacgc cctgcacgct
   167701 taccgcgcct gtatgaatat ggggacgtta agcggtgcgc cgaaagtacg cgctatgcag
   167761 ttaattgccg aggctgaagg tcgtcgccgc ggcagctacg gcggcgcggt aggttatttc
   167821 accgcgcacg gcgatctcga cacctgcatt gtgatccgct cggcgctggt ggaaaacggt
   167881 atcgctaccg tgcaagccgg tgctggcgta gtccttgatt ctgttccgca gtcggaagcc
   167941 gacgaaaccc gtaataaagc ccgcgctgta ctgcgcgcta ttgccaccgc gcatcatgca
   168001 caggagacgt tctaatggct gacattctgc tgctcgataa tatcgactct tttacttaca
   168061 acctggcaga tcagttgcgc agcaatggtc ataacgtggt gatttaccgc aaccatattc
   168121 cggcgcagac cttaattgaa cgccttgcga cgatgagcaa tccggtgctg atgctttctc
   168181 ctggacccgg tgtgccgagc gaagctggtt gtatgcctga actgcttacc cgcctgcgcg
   168241 gtaagctgcc tattattggc atttgccttg gtcatcaggc gattgtcgaa gcttacgggg
   168301 gctatgtcgg tcaggcgggc gaaattcttc acggtaaagc gtcgagcatt gaacatgacg
   168361 gtcaggcgat gtttgccgga ttaacaaacc cgctgccggt ggcgcgttat cactcgctgg
   168421 ttggcagtaa tattccggcc ggtttaacca tcaacgccca ttttaatggc atggtgatgg
   168481 cggtgcgtca cgatgcagat cgcgtttgcg gattccagtt ccacccggaa tccattctca
   168541 ccacccaggg cgctcgcctg ctggaacaaa cgctggcctg ggcgcagcag aaactagagc
   168601 caaccaacac gctgcaaccg attctggaaa aactgtatca ggcgcagacc cttagccagc
   168661 aggaaagtca tcagctattt tcagcggtgg tacgtggtga actgaaaccg gaacacctgg
   168721 cggcggcgct ggtgagcatg aaaattcgcg gcgagcaccc gaacgagatc gccggagcag
   168781 caaccgcgct actggaaaac gccgcgccgt tcccgcgtcc ggattatctg tttgccgata
   168841 tcgtcggcac cggcggtgac ggcagcaaca gcatcaatat ttccaccgcc agtgcgtttg
   168901 tcgccgcggc ctgtgggctg aaagtggcga aacacggcaa ccgtagcgtc tccagtaaat
   168961 ctggctcgtc ggatctgctg gcggcgttcg gtattaatct tgatatgaac gccgataaat
   169021 cgcgccaggc gctggatgag ttaggtgtat gtttcctctt tgcaccgaaa tatcacaccg
   169081 gatttcgcca tgcaatgccg gttcgccagc aactaaaaac ccgcaccctg ttcaatgtgc
   169141 tggggccatt gattaacccg gcgcatccgc cgctggcgtt aattggtgtt tatagtccgg
   169201 aactggtgct gccgattgcc gaaaccttac gcgtgctggg gtatcaacgc gcggcggtgg
   169261 tgcacagcgg cgggatggat gaagtttcat tacacgcgcc gacaatcgtt gccgagctgc
   169321 atgacggcga aattaagagc tatcaattga ccgctgaaga ttttggcctg actccctacc
   169381 accaggagca actggcaggc ggaacaccgg aagaaaaccg tgacatttta acacgcttgt
   169441 tacaaggtaa aggcgacgcc gcccatgaag cagccgtcgc ggcgaatgtc gccatgttaa
   169501 tgcgcctgca tggccatgaa gatctgcaag ccaatgcgca aaccgttctt gaggtactgc
   169561 gcagtggttc cgcttacgac agagtcaccg cactggcggc acgagggtaa atgatgcaaa
   169621 ccgttttagc gaaaatcgtc gcagacaagg cgatttgggt agaagcccgc aaacagcagc
   169681 aaccgctggc cagttttcag aatgaggttc agccgagcac gcgacatttt tatgatgcgc
   169741 tacagggtgc gcgcacggcg tttattctgg agtgcaagaa agcgtcgccg tcaaaaggcg
   169801 tgatctgtga tgatttcgat ccagcacgca ttgccgccat ttataaacat tacgcttcgg
   169861 caatttcggt gctgactgat gagaaatatt ttcaggggag ctttgatttc ctccccatcg
   169921 tcagccaaat cgccccgcag ccgattttat gtaaagactt cattatcgac ccttaccaga
   169981 tctatctggc gcgctattac caggccgatg cctgcttatt aatgctttca gtactggatg
   170041 acgaacaata tcgccagctt gccgccgtcg cccacagtct gaagatgggt gtgctgaccg
   170101 aagtcagtaa tgaagaggaa ctggagcgcg ccattgcatt gggggcaaag gtcgttggca
   170161 tcaacaaccg cgatctgcgt gatttgtcga ttgatctcaa ccgtacccgc gagcttgcgc
   170221 cgaaactggg gcacaacgtg acggtaatca gcgaatcagg catcaatact tacgctcagg
   170281 tgcgcgagtt aagccacttc gctgacggtt ttctaattgg ttcggcgttg atggcccatg
   170341 acgatttgca cgccgccgtg cgtcgggtgt tgctgggtga gaataaagta tgtggcctga
   170401 cgcgtgggca agatgctaaa gcagcgtacg acgcgggcgc gatttacggt gggttgattt
   170461 ttgttgcgac atcaccgcgt tgcgtcaacg ttgaacaggc gcaggaagtg atggctgcgg
   170521 caccgttgca gtatgttggc gtgttccgca atcacgatat tgccgatgtg ctggacaaag
   170581 ctaaggtgtt atcactggtg gcagtgcaac tgcatggtaa tgaagatcag ctgtatatcg
   170641 atacgctgcg tgaagctctg ccagcacacg ttgccatctg gaaagcatta agcgtcggtg
   170701 aaaccctgcc cgcccgcgag cttcagcacg ttgataaata tgttttagac aacggccagg
   170761 gtggaagcgg gcaacgtttc gactggtcac tattaaatgg tcaatcgctt ggcaacgttc
   170821 tgctggcggg gggcttaggc gcagataact gcgtggaagc ggcacaaacc ggctgcgccg
   170881 gacttgattt taattctgct gtagagtcgc aaccgggcat caaagacgca cgtcttttgg
   170941 cctcggtttt ccagacgctg cgcgcatatt aaggaaagga acaatgacaa cattacttaa
   171001 cccctatttt ggtgagtttg gcggcatgta cgtgccacaa atcctgatgc ctgctctgcg
   171061 ccagctggaa gaagcttttg tcagcgcgca aaaagatcct gaatttcagg ctcagttcaa
   171121 cgacctgctg aaaaactatg ccgggcgtcc aaccgcgctg accaaatgcc agaacattac
   171181 ggctggcacg aacaccacgc tgtatctgaa gcgcgaagat ttgctgcacg gcggcgcgca
   171241 taaaactaac caggtgctcg gtcaggcttt actggcgaag cggatgggta aaaccgaaat
   171301 catcgccgaa accggggccg gtcagcatgg cgtggcgtcg gcccttgcca gcgccctgct
   171361 cggcctgaaa tgccgtattt atatgggggc caaagacgtt gaacgccagt cgcctaacgt
   171421 ttttcgtatg cgtttaatgg gtgcggaagt gatcccggtg catagcggtt ccgcgacgct
   171481 gaaagatgcc tgtaacgagg cgctgcgcga ctggtccggc agttatgaaa ccgcgcacta
   171541 tatgctgggc accgcagctg gcccacatcc ttatccgacc attgtccgtg agtttcagcg
   171601 gatgattggc gaagaaacca aagcacagat tctggaaaga gaaggtcgcc tgccggatgc
   171661 cgttatcgcc tgtgttggcg gcggttcgaa tgccatcggc atgtttgcag atttcatcaa
   171721 cgaaaccaac gtcggcctga ttggtgtgga gcctggtggt cacggtatcg aaactggcga
   171781 gcacggcgca ccgctaaaac atggtcgcgt gggtatctat ttcggtatga aagcgccgat
   171841 gatgcaaacc gaagacgggc agattgaaga atcttactcc atctccgccg gactcgattt
   171901 tccgtccgtc ggcccacaac acgcgtatct taacagcact ggacgcgctg attatgtgtc
   171961 aattaccgat gatgaagcac tggaagcctt caaaacgctg tgcctgcacg aagggattat
   172021 cccggcgctg gaatcctccc acgccctggc ccatgcgctg aaaatgatgc gcgaaaaccc
   172081 ggaaaaagag cagctactgg tggttaacct ttccggtcgc ggcgataaag acatcttcac
   172141 cgttcacgat attttgaaag cacgagggga aatctgatgg aacgctacga atctctgttt
   172201 gcccagttga aggagcgcaa agaaggcgca ttcgttcctt tcgtcacgct cggtgatccg
   172261 ggcattgagc agtcgctgaa aattatcgat acgctaattg aagccggtgc tgacacgctg
   172321 gagttaggca tccccttctc cgacccactg gcagatggcc cgacgattca aaacgccaca
   172381 ctgcgtgcct ttgcggcagg tgtgaccccg gcgcagtgct ttgagatgct ggcactcatt
   172441 cgccagaaac acccgaccat tcccattggc ctgctgatgt atgccaacct ggtgtttaac
   172501 aaaggcattg atgagtttta tgcccagtgc gagaaagtcg gcgtcgattc ggtgctggtt
   172561 gccgatgtgc cagttgaaga gtccgcgccc ttccgccagg ccgcgttgcg tcataatgtc
   172621 gcacctatct tcatctgccc gccaaatgcc gatgacgacc tgctgcgcca gatagcctct
   172681 tacggtcgtg gttacacata tttgctgtca cgagcgggcg tgaccggcgc agaaaaccgc
   172741 gccgcgttac ccctcaatca tctggttgcg aagctgaaag agtacaacgc ggcacctcca
   172801 ttgcagggat ttggtatttc cgccccggat caggtaaaag cagcgattga tgcaggagct
   172861 gcgggcgcga tttctggttc tgcgattgtt aaaatcatcg agcaacatat taatgagcca
   172921 aagaaaatgc tggcggcact gaaagctttt gtacaaccga tgaaagcggc gacgcgcagt
   172981 taatcccaca gccgccagtt ccgctggcgg cattttaact ttctttaacg aagccggaaa
   173041 agtcccaaat tcatttaata tttatctttt taccgtttcg cttacccctg tcgaacgtca
   173101 acttacgtca tttttccgcc caacagtaat ataatcaaac aatttaatcc cgcaacataa
   173161 caccagtaaa atcaataatt ttctctaagt cacttattcc gcaggcaatt gttaatatat
   173221 caagaacgtt cgtcataata tattttccta ctttcttctc gttacgctta atttgactaa
   173281 tcctcattag cgactaattt taatgagtat cgacacacaa cactcatatt aatgaaacaa
   173341 tgcaacgcaa cgggagaaat aacatggccg aacatcgtgg tggttcagga aatttcgccg
   173401 aagaccgtga gaaggcatcc gacgcaggcc gtaaaggcgg tcagcatagc ggcggtaatt
   173461 ttaaaaatga tccgcaacgc gcatctgaag cgggtaaaaa aggcggtcaa caaagcggtg
   173521 gtaataaatc aggaaaatcc tgattcaccg ttaattatta atatcagctg ataaagataa
   173581 ttgcatctgc gggccgtatg gctcgcagtg atttcactgg agaaaaatat gaatatgaag
   173641 accattgaag atgtatttat tcacctgctt tcagatacct acagcgcaga aaaacaatta
   173701 acccgggcac tggcaaaact cgcaagagca acatcaaacg aaaaattaag tcaggctttt
   173761 catgcgcacc tcgaggaaac acatggacag attgaacgta ttgatcaagt tgtggaatca
   173821 gaatcgaatc tgaaaattaa gcgcatgaaa tgtgtggcaa tggaaggtct tattgaagaa
   173881 gctaatgagg tcatcgaaag taccgagaaa aacgaagtgc gtgatgccgc actgattgcc
   173941 gcagcacaga aagtcgagca ttatgagatt gccagttacg ggacattagc gacgctggct
   174001 gaacaattag gttaccgtaa agcagcgaag cttctgaaag agaccctgga agaagaaaag
   174061 gccaccgaca tcaaactgac tgatctggcc cttaataacg taaataagaa agccgaaaat
   174121 aaagcctgaa atatgaactt taacttttgg tcattttata aagaggacat tttcatgaat
   174181 cgtattgaac attatcatga ctggttacgt gacgcccatg caatggaaaa gcaagccgaa
   174241 tctatgcttg aatccatggc cagccgtata gataattatc ctgaactacg tgctcgtatt
   174301 gaacaacatc ttagtgaaac caaaaaccag attgttcaac tggaaactat tcttgatcgt
   174361 aatgacattt cacgttcagt cattaaagat tccatgagta aaatggctgc gcttgggcag
   174421 tcaatcggtg gtatattccc ttctgatgaa atagtcaaag gctctattag cggatatgtc
   174481 ttcgagcaat ttgaaatcgc ctgttacacc tctctattag cagcagcaaa aaatgccggt
   174541 gatacagcct caattccgat catcgaagcg attttaaatg aggaaaagca aatggccgac
   174601 tggctgattc agcatattcc gcaaacaact gagaaatttt taattcgctc tgaaactgat
   174661 ggcgtagaag cgaagaaata atacccttta tgccatgtcc ttattgaccc cgtatattac
   174721 ggggtcgttt ttgtgcggaa ttaaaaacga tatcctgctg agaacataaa cacccacgga
   174781 tcgaggcgta cgctatcatg ttgctgtgca ccgcccagtt tataattggc ggtggtatcg
   174841 atatccatgt accacactgg tagattttac aaaactcaat aacatattat atttaaagga
   174901 aaaatacaga atttacgatg cttttttata cagatctctc gtgtttttaa ctgattgatt
   174961 ttgttgaact ctttttttgt tttgcaggag ttactcctct taccttattg tccactcgat
   175021 gaccaacaca ttcacaaccc aaaccagaat atcctatcta tgaagcatgt atccgcacgc
   175081 agattaatta tggtttacat tgttctacca caatagtcgc atcctaaatg ggtgcggttg
   175141 aggatcacca tccttgaaga ggatgcccgc cactttaact gcatgacagg gctatttaaa
   175201 caatcactaa aaatcgccca agagcattct gtttctataa tgtattccaa gagattaaaa
   175261 aataatatca tacgttattc ggcagagttc gtatgtacta cggcgtccca accatgtccc
   175321 aaaacaaaaa tttctgccct atacacgttc caaaaaattc aacagcaaag agagtctgct
   175381 gacgagttgt tgattcactg attaatacgt aaaattgata actgctggaa atcatacaat
   175441 actcgtactt tcgaaagttc gctaaccagg tgctacacgc gaggtgttgc agttttcttt
   175501 acgatttatc attcaatcct aatccatgca tgacatgtgg tgcctgtggg gtgatcctac
   175561 ctacgtaatg tggacacagc cctaatcaag gttctgtttt tcaaaacagt tccgtgctga
   175621 gacagccaca agtactgtgt cggcaccacc gattgtaatc acactagatc taattaaaca
   175681 cagccgtctg cattatttcc cgactcataa aatattagcc gtaaatgcat tcgactttta
   175741 gtgagtggaa gaaactttcc gccctagcat tatcgtaaca gcgccccttt gcactcatcc
   175801 tgtcatgcag gtatgccgct tcagaagcgc ctgataatgc gttgaaatca ggacggtggc
   175861 tacttgctat tcagttcacg atacatccct taaggaccag cgaatagcaa tgagtcactt
   175921 tattatcaag tttgatgacc gctttgatgc taacttttaa taagagtctc ttcacacaaa
   175981 atcgtgtacc gagtctacac tgaataacaa tcactcctca ccgtaaagtg accgatactg
   176041 caatcaaact cacactgctc tctggccaca agcattgata atgttattgg ctctctgctt
   176101 agtgggtgat gcatcccatc attaattata cgagaaagag ccatttcatc atataaacta
   176161 catactctgg aaccttcagc atttcttaaa aaaacacctc gttg
//
LOCUS       DABSYT020000002       116863 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.002, whole genome shotgun sequence.
ACCESSION   DABSYT020000002 DABSYT020000000
VERSION     DABSYT020000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 116863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 116863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 116863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..116863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.002"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1233)
                     /gene="pitB"
                     /locus_tag="ID725_RS00855"
                     /old_locus_tag="pgaptmp_000171"
     CDS             complement(<1..1233)
                     /gene="pitB"
                     /locus_tag="ID725_RS00855"
                     /old_locus_tag="pgaptmp_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HGV7848856.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPGNTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGE"
     gene            complement(1525..3384)
                     /gene="gss"
                     /locus_tag="ID725_RS00860"
                     /old_locus_tag="pgaptmp_000172"
     CDS             complement(1525..3384)
                     /gene="gss"
                     /locus_tag="ID725_RS00860"
                     /old_locus_tag="pgaptmp_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGV7848857.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            3589..4455
                     /gene="yghU"
                     /locus_tag="ID725_RS00865"
                     /old_locus_tag="pgaptmp_000173"
     CDS             3589..4455
                     /gene="yghU"
                     /locus_tag="ID725_RS00865"
                     /old_locus_tag="pgaptmp_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGV7848858.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(4578..4826)
                     /gene="hybG"
                     /locus_tag="ID725_RS00870"
                     /old_locus_tag="pgaptmp_000174"
     CDS             complement(4578..4826)
                     /gene="hybG"
                     /locus_tag="ID725_RS00870"
                     /old_locus_tag="pgaptmp_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGV7848859.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(4839..5180)
                     /gene="hypA"
                     /locus_tag="ID725_RS00875"
                     /old_locus_tag="pgaptmp_000175"
     CDS             complement(4839..5180)
                     /gene="hypA"
                     /locus_tag="ID725_RS00875"
                     /old_locus_tag="pgaptmp_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGV7848860.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5173..5661)
                     /gene="hybE"
                     /locus_tag="ID725_RS00880"
                     /old_locus_tag="pgaptmp_000176"
     CDS             complement(5173..5661)
                     /gene="hybE"
                     /locus_tag="ID725_RS00880"
                     /old_locus_tag="pgaptmp_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGV7848861.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(5654..6148)
                     /gene="hybD"
                     /locus_tag="ID725_RS00885"
                     /old_locus_tag="pgaptmp_000177"
     CDS             complement(5654..6148)
                     /gene="hybD"
                     /locus_tag="ID725_RS00885"
                     /old_locus_tag="pgaptmp_000177"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGV7848862.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6148..7851)
                     /gene="hybC"
                     /locus_tag="ID725_RS00890"
                     /old_locus_tag="pgaptmp_000178"
     CDS             complement(6148..7851)
                     /gene="hybC"
                     /locus_tag="ID725_RS00890"
                     /old_locus_tag="pgaptmp_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGV7848863.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(7848..9026)
                     /gene="hybB"
                     /locus_tag="ID725_RS00895"
                     /old_locus_tag="pgaptmp_000179"
     CDS             complement(7848..9026)
                     /gene="hybB"
                     /locus_tag="ID725_RS00895"
                     /old_locus_tag="pgaptmp_000179"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGV7848864.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9016..10002)
                     /gene="hybA"
                     /locus_tag="ID725_RS00900"
                     /old_locus_tag="pgaptmp_000180"
     CDS             complement(9016..10002)
                     /gene="hybA"
                     /locus_tag="ID725_RS00900"
                     /old_locus_tag="pgaptmp_000180"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGV7848865.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10005..11123)
                     /gene="hybO"
                     /locus_tag="ID725_RS00905"
                     /old_locus_tag="pgaptmp_000181"
     CDS             complement(10005..11123)
                     /gene="hybO"
                     /locus_tag="ID725_RS00905"
                     /old_locus_tag="pgaptmp_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGV7848866.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(11312..11599)
                     /gene="yghW"
                     /locus_tag="ID725_RS00910"
                     /old_locus_tag="pgaptmp_000182"
     CDS             complement(11312..11599)
                     /gene="yghW"
                     /locus_tag="ID725_RS00910"
                     /old_locus_tag="pgaptmp_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGV7848867.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(11718..12605)
                     /gene="yghX"
                     /locus_tag="ID725_RS00915"
                     /old_locus_tag="pgaptmp_000183"
     CDS             complement(11718..12605)
                     /gene="yghX"
                     /locus_tag="ID725_RS00915"
                     /old_locus_tag="pgaptmp_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGV7848868.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRILKWFDKYLS"
     gene            12762..13802
                     /gene="gpr"
                     /locus_tag="ID725_RS00920"
                     /old_locus_tag="pgaptmp_000184"
     CDS             12762..13802
                     /gene="gpr"
                     /locus_tag="ID725_RS00920"
                     /old_locus_tag="pgaptmp_000184"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGV7848869.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(13842..14336)
                     /gene="yqhA"
                     /locus_tag="ID725_RS00925"
                     /old_locus_tag="pgaptmp_000185"
     CDS             complement(13842..14336)
                     /gene="yqhA"
                     /locus_tag="ID725_RS00925"
                     /old_locus_tag="pgaptmp_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGV7848870.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            14527..15411
                     /gene="yghA"
                     /locus_tag="ID725_RS00930"
                     /old_locus_tag="pgaptmp_000186"
     CDS             14527..15411
                     /gene="yghA"
                     /locus_tag="ID725_RS00930"
                     /old_locus_tag="pgaptmp_000186"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGV7848871.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(15683..16108)
                     /gene="exbD"
                     /locus_tag="ID725_RS00935"
                     /old_locus_tag="pgaptmp_000187"
     CDS             complement(15683..16108)
                     /gene="exbD"
                     /locus_tag="ID725_RS00935"
                     /old_locus_tag="pgaptmp_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGV7848872.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16115..16849)
                     /gene="exbB"
                     /locus_tag="ID725_RS00940"
                     /old_locus_tag="pgaptmp_000188"
     CDS             complement(16115..16849)
                     /gene="exbB"
                     /locus_tag="ID725_RS00940"
                     /old_locus_tag="pgaptmp_000188"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGV7848873.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17101..18288
                     /gene="metC"
                     /locus_tag="ID725_RS00945"
                     /old_locus_tag="pgaptmp_000189"
     CDS             17101..18288
                     /gene="metC"
                     /locus_tag="ID725_RS00945"
                     /old_locus_tag="pgaptmp_000189"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGV7848874.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            18428..19087
                     /gene="yghB"
                     /locus_tag="ID725_RS00950"
                     /old_locus_tag="pgaptmp_000190"
     CDS             18428..19087
                     /gene="yghB"
                     /locus_tag="ID725_RS00950"
                     /old_locus_tag="pgaptmp_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGV7848875.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19127..20026)
                     /gene="yqhC"
                     /locus_tag="ID725_RS00955"
                     /old_locus_tag="pgaptmp_000191"
     CDS             complement(19127..20026)
                     /gene="yqhC"
                     /locus_tag="ID725_RS00955"
                     /old_locus_tag="pgaptmp_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGV7848876.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20220..21383
                     /gene="yqhD"
                     /locus_tag="ID725_RS00960"
                     /old_locus_tag="pgaptmp_000192"
     CDS             20220..21383
                     /gene="yqhD"
                     /locus_tag="ID725_RS00960"
                     /old_locus_tag="pgaptmp_000192"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGV7848877.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            21488..22315
                     /gene="dkgA"
                     /locus_tag="ID725_RS00965"
                     /old_locus_tag="pgaptmp_000193"
     CDS             21488..22315
                     /gene="dkgA"
                     /locus_tag="ID725_RS00965"
                     /old_locus_tag="pgaptmp_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGV7848878.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            22515..23437
                     /gene="yqhG"
                     /locus_tag="ID725_RS00970"
                     /old_locus_tag="pgaptmp_000194"
                     /pseudo
     CDS             22515..23437
                     /gene="yqhG"
                     /locus_tag="ID725_RS00970"
                     /old_locus_tag="pgaptmp_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            23486..23743
                     /gene="yqhH"
                     /locus_tag="ID725_RS00975"
                     /old_locus_tag="pgaptmp_000195"
     CDS             23486..23743
                     /gene="yqhH"
                     /locus_tag="ID725_RS00975"
                     /old_locus_tag="pgaptmp_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGV7848879.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(23786..26005)
                     /gene="ygiQ"
                     /locus_tag="ID725_RS00980"
                     /old_locus_tag="pgaptmp_000196"
     CDS             complement(23786..26005)
                     /gene="ygiQ"
                     /locus_tag="ID725_RS00980"
                     /old_locus_tag="pgaptmp_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGV7848880.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26116..27528)
                     /gene="ftsP"
                     /locus_tag="ID725_RS00985"
                     /old_locus_tag="pgaptmp_000197"
     CDS             complement(26116..27528)
                     /gene="ftsP"
                     /locus_tag="ID725_RS00985"
                     /old_locus_tag="pgaptmp_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGV7848881.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(27603..28340)
                     /gene="plsC"
                     /locus_tag="ID725_RS00990"
                     /old_locus_tag="pgaptmp_000198"
                     /gene_synonym="parF"
     CDS             complement(27603..28340)
                     /gene="plsC"
                     /locus_tag="ID725_RS00990"
                     /old_locus_tag="pgaptmp_000198"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGV7848882.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(28574..30832)
                     /gene="parC"
                     /locus_tag="ID725_RS00995"
                     /old_locus_tag="pgaptmp_000199"
     CDS             complement(28574..30832)
                     /gene="parC"
                     /locus_tag="ID725_RS00995"
                     /old_locus_tag="pgaptmp_000199"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGV7848883.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30970..32577)
                     /gene="ygiS"
                     /locus_tag="ID725_RS01000"
                     /old_locus_tag="pgaptmp_000200"
     CDS             complement(30970..32577)
                     /gene="ygiS"
                     /locus_tag="ID725_RS01000"
                     /old_locus_tag="pgaptmp_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGV7848884.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(32686..33168)
                     /gene="ygiV"
                     /locus_tag="ID725_RS01005"
                     /old_locus_tag="pgaptmp_000201"
     CDS             complement(32686..33168)
                     /gene="ygiV"
                     /locus_tag="ID725_RS01005"
                     /old_locus_tag="pgaptmp_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGV7848885.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(33221..33613)
                     /gene="ygiW"
                     /locus_tag="ID725_RS01010"
                     /old_locus_tag="pgaptmp_000202"
     CDS             complement(33221..33613)
                     /gene="ygiW"
                     /locus_tag="ID725_RS01010"
                     /old_locus_tag="pgaptmp_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGV7848886.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33765..34424
                     /gene="qseB"
                     /locus_tag="ID725_RS01015"
                     /old_locus_tag="pgaptmp_000203"
     CDS             33765..34424
                     /gene="qseB"
                     /locus_tag="ID725_RS01015"
                     /old_locus_tag="pgaptmp_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGV7848887.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            34421..35770
                     /gene="qseC"
                     /locus_tag="ID725_RS01020"
                     /old_locus_tag="pgaptmp_000204"
     CDS             34421..35770
                     /gene="qseC"
                     /locus_tag="ID725_RS01020"
                     /old_locus_tag="pgaptmp_000204"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGV7848888.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35880..36461
                     /gene="mdaB"
                     /locus_tag="ID725_RS01025"
                     /old_locus_tag="pgaptmp_000205"
     CDS             35880..36461
                     /gene="mdaB"
                     /locus_tag="ID725_RS01025"
                     /old_locus_tag="pgaptmp_000205"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGV7848889.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            36492..36806
                     /gene="ygiN"
                     /locus_tag="ID725_RS01030"
                     /old_locus_tag="pgaptmp_000206"
     CDS             36492..36806
                     /gene="ygiN"
                     /locus_tag="ID725_RS01030"
                     /old_locus_tag="pgaptmp_000206"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGV7848890.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36851..37738)
                     /locus_tag="ID725_RS01035"
                     /old_locus_tag="pgaptmp_000207"
     CDS             complement(36851..37738)
                     /locus_tag="ID725_RS01035"
                     /old_locus_tag="pgaptmp_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGV7848891.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(37735..38682)
                     /locus_tag="ID725_RS01040"
                     /old_locus_tag="pgaptmp_000208"
     CDS             complement(37735..38682)
                     /locus_tag="ID725_RS01040"
                     /old_locus_tag="pgaptmp_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGV7848892.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(38693..39730)
                     /locus_tag="ID725_RS01045"
                     /old_locus_tag="pgaptmp_000209"
     CDS             complement(38693..39730)
                     /locus_tag="ID725_RS01045"
                     /old_locus_tag="pgaptmp_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGV7848893.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(39739..40722)
                     /locus_tag="ID725_RS01050"
                     /old_locus_tag="pgaptmp_000210"
     CDS             complement(39739..40722)
                     /locus_tag="ID725_RS01050"
                     /old_locus_tag="pgaptmp_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGV7848894.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(40719..41528)
                     /locus_tag="ID725_RS01055"
                     /old_locus_tag="pgaptmp_000211"
     CDS             complement(40719..41528)
                     /locus_tag="ID725_RS01055"
                     /old_locus_tag="pgaptmp_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGV7848895.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
     gene            41902..44043
                     /locus_tag="ID725_RS01060"
                     /old_locus_tag="pgaptmp_000212"
     CDS             41902..44043
                     /locus_tag="ID725_RS01060"
                     /old_locus_tag="pgaptmp_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HGV7848896.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(44107..45999)
                     /gene="parE"
                     /locus_tag="ID725_RS01065"
                     /old_locus_tag="pgaptmp_000213"
     CDS             complement(44107..45999)
                     /gene="parE"
                     /locus_tag="ID725_RS01065"
                     /old_locus_tag="pgaptmp_000213"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGV7848897.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(46028..46609)
                     /gene="yqiA"
                     /locus_tag="ID725_RS01070"
                     /old_locus_tag="pgaptmp_000214"
     CDS             complement(46028..46609)
                     /gene="yqiA"
                     /locus_tag="ID725_RS01070"
                     /old_locus_tag="pgaptmp_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGV7848898.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(46609..47436)
                     /gene="cpdA"
                     /locus_tag="ID725_RS01075"
                     /old_locus_tag="pgaptmp_000215"
     CDS             complement(46609..47436)
                     /gene="cpdA"
                     /locus_tag="ID725_RS01075"
                     /old_locus_tag="pgaptmp_000215"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGV7848899.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(47461..47883)
                     /gene="yqiB"
                     /locus_tag="ID725_RS01080"
                     /old_locus_tag="pgaptmp_000216"
     CDS             complement(47461..47883)
                     /gene="yqiB"
                     /locus_tag="ID725_RS01080"
                     /old_locus_tag="pgaptmp_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGV7848900.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(47884..48513)
                     /gene="nudF"
                     /locus_tag="ID725_RS01085"
                     /old_locus_tag="pgaptmp_000217"
     CDS             complement(47884..48513)
                     /gene="nudF"
                     /locus_tag="ID725_RS01085"
                     /old_locus_tag="pgaptmp_000217"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGV7848901.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            48718..50199
                     /gene="tolC"
                     /locus_tag="ID725_RS01090"
                     /old_locus_tag="pgaptmp_000218"
     CDS             48718..50199
                     /gene="tolC"
                     /locus_tag="ID725_RS01090"
                     /old_locus_tag="pgaptmp_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGV7848902.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            50347..51018
                     /gene="ygiB"
                     /locus_tag="ID725_RS01095"
                     /old_locus_tag="pgaptmp_000219"
     CDS             50347..51018
                     /gene="ygiB"
                     /locus_tag="ID725_RS01095"
                     /old_locus_tag="pgaptmp_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGV7848903.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            51024..52184
                     /gene="ygiC"
                     /locus_tag="ID725_RS01100"
                     /old_locus_tag="pgaptmp_000220"
     CDS             51024..52184
                     /gene="ygiC"
                     /locus_tag="ID725_RS01100"
                     /old_locus_tag="pgaptmp_000220"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGV7848904.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(52222..53010)
                     /gene="ygiD"
                     /locus_tag="ID725_RS01105"
                     /old_locus_tag="pgaptmp_000221"
     CDS             complement(52222..53010)
                     /gene="ygiD"
                     /locus_tag="ID725_RS01105"
                     /old_locus_tag="pgaptmp_000221"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGV7848905.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            53153..53926
                     /gene="zupT"
                     /locus_tag="ID725_RS01110"
                     /old_locus_tag="pgaptmp_000222"
     CDS             53153..53926
                     /gene="zupT"
                     /locus_tag="ID725_RS01110"
                     /old_locus_tag="pgaptmp_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGV7848906.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(53984..54154)
                     /gene="yqiD"
                     /locus_tag="ID725_RS01115"
                     /old_locus_tag="pgaptmp_000223"
     CDS             complement(53984..54154)
                     /gene="yqiD"
                     /locus_tag="ID725_RS01115"
                     /old_locus_tag="pgaptmp_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGV7848907.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(54416..55069)
                     /gene="ribB"
                     /locus_tag="ID725_RS01120"
                     /old_locus_tag="pgaptmp_000224"
     CDS             complement(54416..55069)
                     /gene="ribB"
                     /locus_tag="ID725_RS01120"
                     /old_locus_tag="pgaptmp_000224"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGV7848908.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(55173..55321)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            55443..55742
                     /gene="ubiK"
                     /locus_tag="ID725_RS01125"
                     /old_locus_tag="pgaptmp_000225"
     CDS             55443..55742
                     /gene="ubiK"
                     /locus_tag="ID725_RS01125"
                     /old_locus_tag="pgaptmp_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGV7848909.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(55777..55977)
                     /gene="glgS"
                     /locus_tag="ID725_RS01130"
                     /old_locus_tag="pgaptmp_000226"
     CDS             complement(55777..55977)
                     /gene="glgS"
                     /locus_tag="ID725_RS01130"
                     /old_locus_tag="pgaptmp_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGV7848910.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            56246..56860
                     /gene="yqiJ"
                     /locus_tag="ID725_RS01135"
                     /old_locus_tag="pgaptmp_000227"
     CDS             56246..56860
                     /gene="yqiJ"
                     /locus_tag="ID725_RS01135"
                     /old_locus_tag="pgaptmp_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGV7848911.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            56902..58563
                     /gene="yqiK"
                     /locus_tag="ID725_RS01140"
                     /old_locus_tag="pgaptmp_000228"
     CDS             56902..58563
                     /gene="yqiK"
                     /locus_tag="ID725_RS01140"
                     /old_locus_tag="pgaptmp_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGV7848912.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLKIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            58802..58861
                     /locus_tag="ID725_RS01145"
                     /old_locus_tag="pgaptmp_000229"
     CDS             58802..58861
                     /locus_tag="ID725_RS01145"
                     /old_locus_tag="pgaptmp_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGV7848913.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            59178..59237
                     /gene="ibsE"
                     /locus_tag="ID725_RS01150"
                     /old_locus_tag="pgaptmp_000230"
     CDS             59178..59237
                     /gene="ibsE"
                     /locus_tag="ID725_RS01150"
                     /old_locus_tag="pgaptmp_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGV7848914.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(59357..60790)
                     /gene="hldE"
                     /locus_tag="ID725_RS01155"
                     /old_locus_tag="pgaptmp_000231"
     CDS             complement(59357..60790)
                     /gene="hldE"
                     /locus_tag="ID725_RS01155"
                     /old_locus_tag="pgaptmp_000231"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGV7848915.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(60838..63678)
                     /gene="glnE"
                     /locus_tag="ID725_RS01160"
                     /old_locus_tag="pgaptmp_000232"
     CDS             complement(60838..63678)
                     /gene="glnE"
                     /locus_tag="ID725_RS01160"
                     /old_locus_tag="pgaptmp_000232"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGV7848916.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRWELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(63701..65002)
                     /gene="ygiF"
                     /locus_tag="ID725_RS01165"
                     /old_locus_tag="pgaptmp_000233"
     CDS             complement(63701..65002)
                     /gene="ygiF"
                     /locus_tag="ID725_RS01165"
                     /old_locus_tag="pgaptmp_000233"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGV7848917.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            65244..65864
                     /gene="ygiM"
                     /locus_tag="ID725_RS01170"
                     /old_locus_tag="pgaptmp_000234"
     CDS             65244..65864
                     /gene="ygiM"
                     /locus_tag="ID725_RS01170"
                     /old_locus_tag="pgaptmp_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGV7848918.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            65928..67166
                     /gene="cca"
                     /locus_tag="ID725_RS01175"
                     /old_locus_tag="pgaptmp_000235"
     CDS             65928..67166
                     /gene="cca"
                     /locus_tag="ID725_RS01175"
                     /old_locus_tag="pgaptmp_000235"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGV7848919.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(67347..68168)
                     /gene="bacA"
                     /locus_tag="ID725_RS01180"
                     /old_locus_tag="pgaptmp_000236"
     CDS             complement(67347..68168)
                     /gene="bacA"
                     /locus_tag="ID725_RS01180"
                     /old_locus_tag="pgaptmp_000236"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGV7848920.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(68259..68627)
                     /gene="folB"
                     /locus_tag="ID725_RS01185"
                     /old_locus_tag="pgaptmp_000237"
     CDS             complement(68259..68627)
                     /gene="folB"
                     /locus_tag="ID725_RS01185"
                     /old_locus_tag="pgaptmp_000237"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGV7848921.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            68733..69350
                     /gene="plsY"
                     /locus_tag="ID725_RS01190"
                     /old_locus_tag="pgaptmp_000238"
     CDS             68733..69350
                     /gene="plsY"
                     /locus_tag="ID725_RS01190"
                     /old_locus_tag="pgaptmp_000238"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGV7848922.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(69363..70295)
                     /gene="ttdR"
                     /locus_tag="ID725_RS01195"
                     /old_locus_tag="pgaptmp_000239"
                     /gene_synonym="dan"
     CDS             complement(69363..70295)
                     /gene="ttdR"
                     /locus_tag="ID725_RS01195"
                     /old_locus_tag="pgaptmp_000239"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGV7848923.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            70502..71413
                     /gene="ttdA"
                     /locus_tag="ID725_RS01200"
                     /old_locus_tag="pgaptmp_000240"
     CDS             70502..71413
                     /gene="ttdA"
                     /locus_tag="ID725_RS01200"
                     /old_locus_tag="pgaptmp_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGV7848924.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            71410..72015
                     /gene="ttdB"
                     /locus_tag="ID725_RS01205"
                     /old_locus_tag="pgaptmp_000241"
     CDS             71410..72015
                     /gene="ttdB"
                     /locus_tag="ID725_RS01205"
                     /old_locus_tag="pgaptmp_000241"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGV7848925.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            72064..73527
                     /gene="ttdT"
                     /locus_tag="ID725_RS01210"
                     /old_locus_tag="pgaptmp_000242"
     CDS             72064..73527
                     /gene="ttdT"
                     /locus_tag="ID725_RS01210"
                     /old_locus_tag="pgaptmp_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGV7848926.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(73570..74583)
                     /gene="tsaD"
                     /locus_tag="ID725_RS01215"
                     /old_locus_tag="pgaptmp_000243"
     CDS             complement(73570..74583)
                     /gene="tsaD"
                     /locus_tag="ID725_RS01215"
                     /old_locus_tag="pgaptmp_000243"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGV7848927.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            74821..75036
                     /gene="rpsU"
                     /locus_tag="ID725_RS01220"
                     /old_locus_tag="pgaptmp_000244"
     CDS             74821..75036
                     /gene="rpsU"
                     /locus_tag="ID725_RS01220"
                     /old_locus_tag="pgaptmp_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGV7848928.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            75147..76892
                     /gene="dnaG"
                     /locus_tag="ID725_RS01225"
                     /old_locus_tag="pgaptmp_000245"
     CDS             75147..76892
                     /gene="dnaG"
                     /locus_tag="ID725_RS01225"
                     /old_locus_tag="pgaptmp_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGV7848929.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            77087..78928
                     /gene="rpoD"
                     /locus_tag="ID725_RS01230"
                     /old_locus_tag="pgaptmp_000246"
     CDS             77087..78928
                     /gene="rpoD"
                     /locus_tag="ID725_RS01230"
                     /old_locus_tag="pgaptmp_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGV7848930.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(79007..79513)
                     /gene="mug"
                     /locus_tag="ID725_RS01235"
                     /old_locus_tag="pgaptmp_000247"
     CDS             complement(79007..79513)
                     /gene="mug"
                     /locus_tag="ID725_RS01235"
                     /old_locus_tag="pgaptmp_000247"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGV7848931.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            79638..79713
                     /locus_tag="ID725_RS01240"
                     /old_locus_tag="pgaptmp_000248"
     tRNA            79638..79713
                     /locus_tag="ID725_RS01240"
                     /old_locus_tag="pgaptmp_000248"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:79671..79673,aa:Ile,seq:cat)
     gene            complement(79767..80531)
                     /gene="nfeF"
                     /locus_tag="ID725_RS01245"
                     /old_locus_tag="pgaptmp_000249"
                     /gene_synonym="yqjH"
     CDS             complement(79767..80531)
                     /gene="nfeF"
                     /locus_tag="ID725_RS01245"
                     /old_locus_tag="pgaptmp_000249"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGV7848932.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            80819..81442
                     /gene="nfeR"
                     /locus_tag="ID725_RS01250"
                     /old_locus_tag="pgaptmp_000250"
                     /gene_synonym="yqjI"
     CDS             80819..81442
                     /gene="nfeR"
                     /locus_tag="ID725_RS01250"
                     /old_locus_tag="pgaptmp_000250"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGV7848933.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(81596..83116)
                     /gene="aer"
                     /locus_tag="ID725_RS01255"
                     /old_locus_tag="pgaptmp_000251"
     CDS             complement(81596..83116)
                     /gene="aer"
                     /locus_tag="ID725_RS01255"
                     /old_locus_tag="pgaptmp_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGV7848934.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            83534..84913
                     /gene="ygjG"
                     /locus_tag="ID725_RS01260"
                     /old_locus_tag="pgaptmp_000252"
     CDS             83534..84913
                     /gene="ygjG"
                     /locus_tag="ID725_RS01260"
                     /old_locus_tag="pgaptmp_000252"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGV7848935.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(84955..85287)
                     /gene="ygjH"
                     /locus_tag="ID725_RS01265"
                     /old_locus_tag="pgaptmp_000253"
     CDS             complement(84955..85287)
                     /gene="ygjH"
                     /locus_tag="ID725_RS01265"
                     /old_locus_tag="pgaptmp_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGV7848936.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            85506..86489
                     /gene="ebgR"
                     /locus_tag="ID725_RS01270"
                     /old_locus_tag="pgaptmp_000254"
     CDS             85506..86489
                     /gene="ebgR"
                     /locus_tag="ID725_RS01270"
                     /old_locus_tag="pgaptmp_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGV7848937.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            86673..89765
                     /gene="ebgA"
                     /locus_tag="ID725_RS01275"
                     /old_locus_tag="pgaptmp_000255"
     CDS             86673..89765
                     /gene="ebgA"
                     /locus_tag="ID725_RS01275"
                     /old_locus_tag="pgaptmp_000255"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGV7848938.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            89762..90211
                     /gene="ebgC"
                     /locus_tag="ID725_RS01280"
                     /old_locus_tag="pgaptmp_000256"
     CDS             89762..90211
                     /gene="ebgC"
                     /locus_tag="ID725_RS01280"
                     /old_locus_tag="pgaptmp_000256"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGV7848939.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            90274..91707
                     /gene="ygjI"
                     /locus_tag="ID725_RS01285"
                     /old_locus_tag="pgaptmp_000257"
     CDS             90274..91707
                     /gene="ygjI"
                     /locus_tag="ID725_RS01285"
                     /old_locus_tag="pgaptmp_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGV7848940.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            91841..92911
                     /gene="ygjJ"
                     /locus_tag="ID725_RS01290"
                     /old_locus_tag="pgaptmp_000258"
     CDS             91841..92911
                     /gene="ygjJ"
                     /locus_tag="ID725_RS01290"
                     /old_locus_tag="pgaptmp_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGV7848941.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            92928..95279
                     /gene="ygjK"
                     /locus_tag="ID725_RS01295"
                     /old_locus_tag="pgaptmp_000259"
     CDS             92928..95279
                     /gene="ygjK"
                     /locus_tag="ID725_RS01295"
                     /old_locus_tag="pgaptmp_000259"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGV7848942.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            95805..97823
                     /gene="fadH"
                     /locus_tag="ID725_RS01300"
                     /old_locus_tag="pgaptmp_000260"
     CDS             95805..97823
                     /gene="fadH"
                     /locus_tag="ID725_RS01300"
                     /old_locus_tag="pgaptmp_000260"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGV7848943.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(97868..98284)
                     /gene="higA"
                     /locus_tag="ID725_RS01305"
                     /old_locus_tag="pgaptmp_000261"
     CDS             complement(97868..98284)
                     /gene="higA"
                     /locus_tag="ID725_RS01305"
                     /old_locus_tag="pgaptmp_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGV7848944.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(98281..98595)
                     /gene="higB"
                     /locus_tag="ID725_RS01310"
                     /old_locus_tag="pgaptmp_000262"
     CDS             complement(98281..98595)
                     /gene="higB"
                     /locus_tag="ID725_RS01310"
                     /old_locus_tag="pgaptmp_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HGV7848945.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(98879..100015)
                     /gene="rlmG"
                     /locus_tag="ID725_RS01315"
                     /old_locus_tag="pgaptmp_000263"
     CDS             complement(98879..100015)
                     /gene="rlmG"
                     /locus_tag="ID725_RS01315"
                     /old_locus_tag="pgaptmp_000263"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGV7848946.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            100100..100603
                     /gene="ygjP"
                     /locus_tag="ID725_RS01320"
                     /old_locus_tag="pgaptmp_000264"
     CDS             100100..100603
                     /gene="ygjP"
                     /locus_tag="ID725_RS01320"
                     /old_locus_tag="pgaptmp_000264"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGV7848947.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            100680..101372
                     /gene="ygjQ"
                     /locus_tag="ID725_RS01325"
                     /old_locus_tag="pgaptmp_000265"
     CDS             100680..101372
                     /gene="ygjQ"
                     /locus_tag="ID725_RS01325"
                     /old_locus_tag="pgaptmp_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGV7848948.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            101451..102437
                     /gene="ygjR"
                     /locus_tag="ID725_RS01330"
                     /old_locus_tag="pgaptmp_000266"
     CDS             101451..102437
                     /gene="ygjR"
                     /locus_tag="ID725_RS01330"
                     /old_locus_tag="pgaptmp_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGV7848949.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            102721..103686
                     /gene="alx"
                     /locus_tag="ID725_RS01335"
                     /old_locus_tag="pgaptmp_000267"
     CDS             102721..103686
                     /gene="alx"
                     /locus_tag="ID725_RS01335"
                     /old_locus_tag="pgaptmp_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGV7848950.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQQVG"
     gene            104085..105329
                     /gene="sstT"
                     /locus_tag="ID725_RS01340"
                     /old_locus_tag="pgaptmp_000268"
     CDS             104085..105329
                     /gene="sstT"
                     /locus_tag="ID725_RS01340"
                     /old_locus_tag="pgaptmp_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGV7848951.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(105334..105885)
                     /gene="ygjV"
                     /locus_tag="ID725_RS01345"
                     /old_locus_tag="pgaptmp_000269"
     CDS             complement(105334..105885)
                     /gene="ygjV"
                     /locus_tag="ID725_RS01345"
                     /old_locus_tag="pgaptmp_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGV7848952.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(105968..107455)
                     /gene="uxaA"
                     /locus_tag="ID725_RS01350"
                     /old_locus_tag="pgaptmp_000270"
     CDS             complement(105968..107455)
                     /gene="uxaA"
                     /locus_tag="ID725_RS01350"
                     /old_locus_tag="pgaptmp_000270"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGV7848953.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(107470..108882)
                     /gene="uxaC"
                     /locus_tag="ID725_RS01355"
                     /old_locus_tag="pgaptmp_000271"
     CDS             complement(107470..108882)
                     /gene="uxaC"
                     /locus_tag="ID725_RS01355"
                     /old_locus_tag="pgaptmp_000271"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGV7848954.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            109365..110663
                     /gene="exuT"
                     /locus_tag="ID725_RS01360"
                     /old_locus_tag="pgaptmp_000272"
     CDS             109365..110663
                     /gene="exuT"
                     /locus_tag="ID725_RS01360"
                     /old_locus_tag="pgaptmp_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGV7848955.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            110793..111569
                     /gene="exuR"
                     /locus_tag="ID725_RS01365"
                     /old_locus_tag="pgaptmp_000273"
     CDS             110793..111569
                     /gene="exuR"
                     /locus_tag="ID725_RS01365"
                     /old_locus_tag="pgaptmp_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGV7848956.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            111809..111892
                     /gene="yqiM"
                     /locus_tag="ID725_RS01370"
                     /old_locus_tag="pgaptmp_000274"
     CDS             111809..111892
                     /gene="yqiM"
                     /locus_tag="ID725_RS01370"
                     /old_locus_tag="pgaptmp_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGV7848957.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            111914..112576
                     /gene="yqjA"
                     /locus_tag="ID725_RS01375"
                     /old_locus_tag="pgaptmp_000275"
     CDS             111914..112576
                     /gene="yqjA"
                     /locus_tag="ID725_RS01375"
                     /old_locus_tag="pgaptmp_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGV7848958.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            112580..112963
                     /gene="mzrA"
                     /locus_tag="ID725_RS01380"
                     /old_locus_tag="pgaptmp_000276"
     CDS             112580..112963
                     /gene="mzrA"
                     /locus_tag="ID725_RS01380"
                     /old_locus_tag="pgaptmp_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGV7848959.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            113110..113478
                     /gene="yqjC"
                     /locus_tag="ID725_RS01385"
                     /old_locus_tag="pgaptmp_000277"
     CDS             113110..113478
                     /gene="yqjC"
                     /locus_tag="ID725_RS01385"
                     /old_locus_tag="pgaptmp_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGV7848960.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            113516..113821
                     /gene="yqjD"
                     /locus_tag="ID725_RS01390"
                     /old_locus_tag="pgaptmp_000278"
     CDS             113516..113821
                     /gene="yqjD"
                     /locus_tag="ID725_RS01390"
                     /old_locus_tag="pgaptmp_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGV7848961.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            113824..114228
                     /gene="yqjE"
                     /locus_tag="ID725_RS01395"
                     /old_locus_tag="pgaptmp_000279"
     CDS             113824..114228
                     /gene="yqjE"
                     /locus_tag="ID725_RS01395"
                     /old_locus_tag="pgaptmp_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGV7848962.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            114218..114517
                     /gene="yqjK"
                     /locus_tag="ID725_RS01400"
                     /old_locus_tag="pgaptmp_000280"
     CDS             114218..114517
                     /gene="yqjK"
                     /locus_tag="ID725_RS01400"
                     /old_locus_tag="pgaptmp_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGV7848963.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            114703..115095
                     /gene="yqjF"
                     /locus_tag="ID725_RS01405"
                     /old_locus_tag="pgaptmp_000281"
     CDS             114703..115095
                     /gene="yqjF"
                     /locus_tag="ID725_RS01405"
                     /old_locus_tag="pgaptmp_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGV7848964.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            115165..116151
                     /gene="yqjG"
                     /locus_tag="ID725_RS01410"
                     /old_locus_tag="pgaptmp_000282"
     CDS             115165..116151
                     /gene="yqjG"
                     /locus_tag="ID725_RS01410"
                     /old_locus_tag="pgaptmp_000282"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGV7848965.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            116443..116808
                     /gene="yhaH"
                     /locus_tag="ID725_RS01415"
                     /old_locus_tag="pgaptmp_000283"
     CDS             116443..116808
                     /gene="yhaH"
                     /locus_tag="ID725_RS01415"
                     /old_locus_tag="pgaptmp_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGV7848966.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT        28622 a        31088 c        29599 g        27554 t
ORIGIN      
        1 ctcaccgatg gtcatcgcaa cacgacgcca gccaatcatg gtgccaatgc cgagcgccag
       61 tgctactgcc atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg
      121 cagttttttc agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc
      181 ggaagtatca gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa
      241 cggctcgtaa ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa
      301 ggtatttccc ggatgacagt gaaactccgt cacttgcgcg ccatcagtag atgccgcagg
      361 caatggaggt tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg
      421 ttcgaagttg gtaacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt
      481 gacgacgaag ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt
      541 ttgtccgtcg ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat
      601 acgcgtccag aatggcggct tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg
      661 gtgaatacgg tcacgctttt tcgttccgct ccagtagcgt cgcagcagga atatcaggcc
      721 tcccgcaatg accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac
      781 ttcacgcagg tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc
      841 gatgccgata atcgcgccaa tcaaggtatg cgaactggag gccggtaaac caaagaacca
      901 cgttcccagg ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaggccgtg
      961 ggttgacccc atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac
     1021 gctaagtccg cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacggcaag
     1081 ctgtggctgc atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa
     1141 accattgatt gcttcgtaga acaacacaaa tgccagagca agtaataaca aaagccctgt
     1201 gtatatatca aggccaacaa ataaatttag catatattag attacgccat tttgaattta
     1261 cgaacggacg cattatcagt gactttaacg gtgtgggcaa agtgaaatat cattttttaa
     1321 cgataaaaag agtgttattt cttgtgtatc agacaataag cattcattaa aaatctttag
     1381 aaataattag aacacaggca ttctgctggt taatgtattc acatatgaaa tgaataaata
     1441 tttgaacata taaatattga atattttgat taatccccgt actgactatt cttcataatc
     1501 agtacgggat tgctacaaca tgtattactc tttcaccaca attaacggtt caatatcact
     1561 ctctttttta ataaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc
     1621 aacggtgaag gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg
     1681 ctgatagatg tttttctgct cggcaaattt accactggtt ttgtccagca cctcttcatg
     1741 atggctgacg aggtcgatgt tgctgccaca gcgaccggcg atcggtttca ctgcgtaacc
     1801 tgttttcacc aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg
     1861 gaacagcgac cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg
     1921 ctcaaagacc agcacttccg ggcgcagcaa tacgtcgata agacgcactt cattttgcgg
     1981 atgaccggta cggatcggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc
     2041 aaacgcggtt tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc
     2101 aatcagttgc ccggcagcat cccagcccag ttcatccagc ccgcgcaaga tacgcgtttc
     2161 aaagcccgcc tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc
     2221 tttgtcctgc atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccag
     2281 ttcgttaatc agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca
     2341 acgttcgagg atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtactcgta
     2401 aaccttcaga ccacgctcat ccatgcagaa atccatacga ccggtgatca tatggtgacg
     2461 gcgacgctgc caggagagac gcaaacgtgg ccagaggatt ttcggaatgt cgaacagcgc
     2521 cagcaggttg tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc
     2581 gttggtggct ttaatcagct cctgctcggc gctctcggta atggtgtagt aatgatacgg
     2641 atcctgattg atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc
     2701 atccagccat ttaccgtcaa actggccttt gttttccaga cgcgctccgc tgattttcag
     2761 cagctcgcct gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat
     2821 ccagcccaga atggtggtgt catcaaaagt gtctttaagg gtatagcagc cgttttcgac
     2881 caccatctcc agttcgcgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt
     2941 ctgttccgca atacggactt tgttgccatg taattgggta atgatggcga catggccagt
     3001 gtctttaaat tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg
     3061 cgagccgtta ggaaatgcct gcaatggcag gatgttgtca ttaaccactt cacgcaggaa
     3121 gcgcagcgag aaaatctccc acgccatacc cacgtcagta aagaccacac cgtagttcag
     3181 aaagagaaaa cggcgagcaa attcaacgca ttgccatttg tggcccatat attcgtcgtc
     3241 gatatagcta cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc
     3301 tgaagagtag attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc
     3361 ctggctggtc gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc
     3421 gtaattgttg tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat
     3481 catacacctc atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa
     3541 gtgacctgct acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat
     3601 acttatcagc ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat
     3661 atcaatcgcc cggtttctgg tccgacgcat gaaaaaacgc tgccggtggg caaacatcca
     3721 ttacaacttt attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag
     3781 ctgctcgcgc tgggcgttac tggcgcagag tacgatgcct ggctgatacg cataggcgat
     3841 ggtgatcaat tctccagcgg ctttgtcgaa gtgaacccaa attcgaagat cccggcgctg
     3901 cgcgatcata cccataatcc gccgatccgc gtgtttgagt caggatcgat tctgctttat
     3961 ctggcggaga aatttggcta cttcctgccg caggatctgg caaaacggac tgaaacgatg
     4021 aactggctgt tctggttaca gggcgcggca ccgttcctcg gcggtggttt tggtcacttt
     4081 taccattacg cgccggtgaa aattgagtac gccatcaatc gctttaccat ggaagccaag
     4141 cgcctgctcg acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag
     4201 tacaccattg cggatatggc gatttggccg tggtttggca acgtggtgct cggcggcgtg
     4261 tatgatgccg cagagtttct ggatgcggga agttataaac atgtgcaacg ctgggcgaaa
     4321 gaagtgggcg aacgtccggc agtgaagcgt gggcgtattg ttaaccgcac caacgggccg
     4381 ctgaacgagc agttgcatga gcgccatgac gccagtgatt ttgagacgaa tacggaagat
     4441 aagcgtcagg ggtaaggatt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt
     4501 tcacgccgca tccggcaatc gagcacaatg cctgatgcga cgctgccgcg tcttatcaga
     4561 cctacgaaag ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct
     4621 aatgtggctt tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc
     4681 acccactggc ccagcagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg
     4741 cgcttgatac cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc
     4801 acctggcctg gaacgccaat acacattgtt aactccggtt attcaacttc aatacttttg
     4861 acgatcagcg aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc
     4921 gcatcgtgct gatgaacctc caccacctgg ctgcaatccc agcaccaggc ctgggcgggt
     4981 ttatagacga tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa
     5041 ctaaaacgga cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg
     5101 gtgacgcgct taacatcgtg ctgctccgcc tgccgctgga taatttcaac ggcgctctgg
     5161 caaagcgaca actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc
     5221 tgcgtgtggt acatccggat tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc
     5281 cgtcaggcgt tggccctctt caatcgacat actgtgcgaa agcggtgaca tcagcgaaca
     5341 ggagaggtat tgcgaaacac cgtccagttc gccaacggta aaggtcatgg tgccatacgg
     5401 cagttgcaga ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg
     5461 gaagataact gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa
     5521 cagcgtaaaa tcagaaacat acaccggcat tgaaggatgc agaaaagaga gagcgtgcat
     5581 cgaacgccgg gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa
     5641 acctgctatc tcttcagtca tgaatcgcct cccgtgggat ggcttccacg ccagattcac
     5701 gcagcgcagc cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta
     5761 agccgatgtg tggctccagc gattccggaa ttacgccgac cagggtcagc tttttcggaa
     5821 actcgccggt gaagcgcagg gccgacaaga cgtcggccag gccaagctga tgcggagaga
     5881 ttttgttggt aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg
     5941 cgctcttctt cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc
     6001 caagcagctc cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt
     6061 atcgttgctc taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat
     6121 tgccgacccc taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc
     6181 gtcagcatcc actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg
     6241 caccacttcc agcggtttat tcggatcagc aaccggtgta cccaccagcg actgctcgta
     6301 aggaccgacg tcatcattga agttacgcgg gccagagttc caggttgatg gaacaactgc
     6361 ctggtagttg ctgatgatac cgtctttgat caccatccag tgagagagca taccgcgcgg
     6421 cgcttcgagg aagccaacac ctttgaactc acccgttgcc ggaatgttcg gcttcacaaa
     6481 ggtggtgtga tcgcctttgc cgatattggt gatcagtgca ctgtattggt tttgcaggat
     6541 atcctgcaat tcacagcagt gaacggtacg accaataata cggcccagcg tggagtgcag
     6601 ctgtgccact tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag
     6661 tttgttctgg gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc
     6721 cacttctacc gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc
     6781 agaccaacca tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc
     6841 tttataccag gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg
     6901 agaagtgatc ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag
     6961 gaagctgccg tttttactgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc
     7021 tttaccgcgc gtcagccatt ccgggtagaa cgcagcgata accgcagtat cgaccttgta
     7081 aacctgctca acaaagtcac tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc
     7141 aaggttcagc acgcccagac cgtcgaggtt gattgggtta gcgaccccac ctaccgccag
     7201 gttctgaatg tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg
     7261 gcactccagc gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt
     7321 catcgccggg tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag
     7381 atctttgatc ttgttctgaa ctttggtgaa ctcttccgga ctgttcaggt gccaggtcga
     7441 aacacctttc agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc
     7501 cacccagtcc agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc
     7561 agccagaatg atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact
     7621 ttctgccgca cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg
     7681 cacaatcatc catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca
     7741 catggtaccg gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg
     7801 taaatgcccc tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc
     7861 gcctcatgac gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg
     7921 agtacgatgt aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc
     7981 caggtcggga agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga
     8041 cgccaggttg cacaacctaa cagcgcgctc agtgctgaca ggaacagcat gcgggaatca
     8101 ttacgcagct tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc
     8161 cagaacatca cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata
     8221 agctcgccaa agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc
     8281 ttgacgaata ggctcttttc atccggaccg ttgccacgca gacccgcctg caccagtgaa
     8341 ccttcaaaga tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc
     8401 aacatttcat agctctgcca cagcggatgc accttgtagc ccgccgagat catcagcgac
     8461 cccattgaag actggtgcat ggtcggcagc agcgcaccga gcgcgatgat gaagaacatc
     8521 accttgttca gacgctgtag cgacaccttc cagcccagac gttcaaacag tgccggagca
     8581 aactccagag ccatcacgcc gatgtagatg gtcatacaga ccgccgtctc gaacagtacc
     8641 gagttcacgt tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg
     8701 tcgatagtga tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga
     8761 cgcaccagcg gatggtattg cccacggtta aagacgtata ccgcccacgc aagcgcccag
     8821 ccgccacagg caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg
     8881 aagccgccgt tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg
     8941 agcatacaga tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg
     9001 cccagcggtt gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt
     9061 gttgcgacga accagcacgg tcaagcccgc cagcacagcc agtggtagca tcatgccttt
     9121 atacagggtg tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg
     9181 caggtcgaga ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc
     9241 tttctcgccg tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga
     9301 tttcagcgtc tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt
     9361 cgcctccgcc atcagctctt cacgcgtacc aaaaatcacc gcgcccgccg ggcacacttc
     9421 tacgcagcca ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc
     9481 gcacttatgc agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca
     9541 ggcgaccatg cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt
     9601 tttcggatct tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca
     9661 gtgcatacac tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc
     9721 tgtgccgctg gtccacactt gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt
     9781 cgaccaggtc tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt
     9841 gacgcaagcc tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg
     9901 aattggcggg cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa
     9961 tgccccgcag gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag
    10021 tcagcgttat ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg
    10081 gcaaccagcc caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc
    10141 tctttagcgt taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc
    10201 tgatggatgc ctttatggaa gccgatacct tcttcgttac agccatagca cgggtgacca
    10261 atcgccaccg gccacacacc gccaacatcg cagaattgca gcgttgagca gttgccgtaa
    10321 gtttctggcc ctttacagcc gaggtggtac aggcaccagc cttcgcggtg gccttcatca
    10381 ccgaactctt tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga
    10441 atcagacggc cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg
    10501 taagtgatga tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga
    10561 atattgataa cggttttgcc tggcagaact tcttgcaagc tgactgcgcc agttgggtta
    10621 actccagctg cggcaacacc gccccacgca gagcaggaac cgatagcgat aatggctgct
    10681 gcgccttccg ccgctttgcg gatgtgatcc acaatcggct caccggcaac catgcaataa
    10741 ataccgttat cttttaatgg gatggaacca tccaccacta acacatactg ccctttgtac
    10801 ttctcgagag cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact
    10861 tcgtgatact ccagagagat agtctccagt acgaggtttt ctaccgttgg atgcgttgca
    10921 cgaagcagag attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga
    10981 cgctgcgggt tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg
    11041 gtggcggcta atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg
    11101 atgagggtgt tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt
    11161 tgcctcaatc gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg
    11221 ttatcgatat catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa
    11281 ttaattaaag caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa
    11341 aaaagcgcaa cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat
    11401 ccagtccata gcggcgcgtc agaatacccg cttcccaggc gctaagctgg cgatctccgg
    11461 tcttttcgta catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga
    11521 attttgtcac attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt
    11581 taccaaaatg gttattcatc gttcacctca ttcgctatat atcgtaatat atagcgatat
    11641 tttaggcaaa gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc
    11701 cgtaagagat aaacctatca ggagagatat ttatcgaacc atttcagtat cctttgccag
    11761 gcaagatcgg cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta
    11821 acccccggat agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa
    11881 gcaggccagc cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct
    11941 tcaatcttcg ccacatcggc agtgggtgcc tgacgaccat aaaacggcac tgcgcaggcc
    12001 agttccgggt aagcgaccgc tgccgcgttc gatacgccac cgccatagca aaatccggta
    12061 atacccactt tgcctgtcgc ttgcggatag cgttgcataa actcaattgc ggcaaagaaa
    12121 tcattcatca gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt
    12181 cccggataac ctccaacgga atttaagccg tcaggtgcca gggcgatata ccccgccttc
    12241 gccactcgcc gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc
    12301 accacggctg gcgttttgcc gctcattttt gcgggtttaa ccagataacc ccgtacctcg
    12361 ccgtgaccat ttggcgaagg atacgtgatg tactcagcaa aaatctccgg gtcggtaaac
    12421 tctacctgag tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc
    12481 atcccgccta ccgcatactt cgccgcaagg ttgaggaact cacgtttcga gattttcccg
    12541 tgagcgtaat agtcgtagta atccagcaac tcttgtggga aatctttggc ggttaaacgc
    12601 ggcatcgttg cactcctcag ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc
    12661 ccagtttttc gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact
    12721 agaaacattg tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc
    12781 ccgaacgtta cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg
    12841 cgttatcgct cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg
    12901 cgatcctgcg taaagcgttt gatttaggca ttacgcactt tgatttagcc aacaattacg
    12961 ggccgcctcc aggaagcgca gaagagaact ttggtcgcct gctgcgggag gattttgccg
    13021 cttatcgcga tgaactgatt atctctacca aagctggcta cgatatgtgg cccggccctt
    13081 acggctctgg cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta
    13141 tggggcttga gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg
    13201 aagaaaccgc ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga
    13261 tctcctctta ctcaccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa
    13321 ttccgctgtt aattcatcaa ccttcgtaca atttactaaa ccgctgggtg gataaaagcg
    13381 gcctgctgga taccctgcaa aataacggcg tgggctgcat tgcctttact cctctggctc
    13441 agggattgct gaccggaaaa tatctcaacg gcattcctga agattcacgg atgcatcgtg
    13501 aagggaataa agttcgtggt ctgacgccga aaatgctcac cgaagccaac ctcaacagcc
    13561 tacgcttatt gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa
    13621 gctggttgct gaaagatgag cgagtgacgt cggtattggt tggtgccagc cgcgcggagc
    13681 aacttgagga gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc
    13741 agatcgatca gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtct tccgataaat
    13801 gacctgttaa taacgggcgc gagaaccgcg cccgaatcag atcagtgatt atgacgagtc
    13861 agtcggtcaa gatagcccat tacaaatgca gagagcacaa acgtcagatg gataatgacg
    13921 taccacatca gtttgttatc agggacattt ttcgcatcca taaagacgcg cagtaagtga
    13981 atggaagaaa ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcatccatt
    14041 ttccccagcc agttcagctt ctctttgttc tcggagatat cgagttgtga gacgaaattc
    14101 tcataaccgg aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc
    14161 gacagcaaca cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga
    14221 ataatctcct ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac
    14281 acgggggcaa gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat
    14341 tcctgtcgtg tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcgcgcaaca
    14401 gcaaatcaac atatccgtta aatctttgtc gcataattct gagggttagg gatcttcctg
    14461 gttcccttat caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga
    14521 aaaacgatgt ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa
    14581 cagaaacaac cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag
    14641 aaaacctatg ttggtagcgg tcgcctgaaa gatcgtaaag ccctggtgac agggggcgat
    14701 tccggaatcg gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc
    14761 agttatcttc ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc
    14821 ggacgcaaag ccgttctgct gccaggcgat ttaagcgatg agaaatttgc ccgttcgctg
    14881 gttcacgaag cgcataaggc cttaggtggg ctggatatta tggcgctggt cgccgggaaa
    14941 caggttgcca ttcctgatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc
    15001 attaacgttt tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt
    15061 gcaagtatta tcaccacttc gtcaatccag gcataccagc caagcccgca tttactggac
    15121 tatgcagcta cgaaggcggc gattctgaac tacagccgag gcctggcaaa acaggtcgcg
    15181 gagaaaggta ttcgggtgaa tattgtcgcg ccaggcccga tctggacagc actgcaaatt
    15241 tccggcggac aaacgcagga taagatcccg cagtttggtc agcaaacgcc gatgaaacgt
    15301 gcggggcaac cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc
    15361 tacgtcaccg cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc
    15421 cggttgtgaa aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac
    15481 gccttatccg cctacaaatg catgaagatt caacatgttg caatagattt tgtaggcctg
    15541 ataagcgtag cgcatcaggc attgagcacc taatgccgga tgcggcgtga acgccttatc
    15601 cggcctacaa acgcacgaag attcaacatg ttgcaataga ttttgtaggc ctgatgaacg
    15661 tagcgcatca ggcaattcta ctttacttcg ctttggcggt ttcttcgccg accagaccta
    15721 tcttcaggta acccgcctga tgcagcgtgt ccattacctt catcaacgtc tcgtaatcga
    15781 cagttttatc cgctcggaag aagatggtgg tgtctttctt gccttcggtt aacgcattca
    15841 acgccgtaat cattgtttca tcggtgaccg gatcgttacc gataaacatc gagttgtctg
    15901 ccttcaccga cagataaacc ggtttttccg ggcgcggctg cggcgtgctg gtagaagcag
    15961 gcaagttaac cttcacatct accgtcgcta acggtgccgc caccataaag ataatcagca
    16021 ggaccaacat cacgtcgata aacggcgtca cgttgatatc atgcatttcg ccgttatcgt
    16081 cgaggttttc gttaagatgc attgccatcg gacattatcc tgcgcgtaat ttttgtgcga
    16141 cacgaaccgg atgcgcagcg gcgctggctt ccagatccag gtcacggctt tgcagcaaca
    16201 atacctgcgc tgcgacatca cccagcatcg ctttaaagcc gccaatctga cgcgcgaata
    16261 cgttatagat aacgaccgcc ggaatcgctg ccaccagacc gattgccgtt gctaacagag
    16321 cttctgcgat acccggcgca acgactgcca ggttagtggt ctgcgtttgc gcgataccaa
    16381 taaagctgtt catgatgccc cagaccgtac caaacagccc aacgaacggc gaaatcgcgc
    16441 cgatggttgc cagatagccg ttaccgcggc ccatttgacg acctactgcg gcgacccgac
    16501 gctccagacg gaagctggta cgttctttaa taccttcgtt atcgtcgctg ccttctgaca
    16561 gttccagctc attctgcgct tcattgagca aatgcaggct taagcttttg ctaccaaaat
    16621 cagcggcgat atcgttggcc tggtttaagg aacgcgcttc agccagcagt tgctgctcgc
    16681 gcttaaggcg acgcttctga ttaaagaact ctacgctctt actgaagaag attgcccagg
    16741 tgactacgga ggccaaaata agcccaatca tcacgcactt aacgacaata tcggcgtgct
    16801 gatacatacc ccagacggaa aggtccgtct gcattaaatt attacccact gtgtatctcc
    16861 aggacgcaag tcacaaaatc tgcgcataat aatatcaaaa cgacgtcgaa ttgatagtcg
    16921 ttctcattac tatttgcata ctgccgtacc tttgctttct tttccttgcg tttacgcagt
    16981 aaaaaagtca ccagcacgcc atttgcgaaa attttctgct ttatgccaat tcttcaggat
    17041 gcgcccgcga atattcatgc tagtttagac atccagacgt ataaaaacag gaatcccgac
    17101 atggcggaca aaaagcttga tactcaactg gtgaatgcag gacgcagcaa aaaatacact
    17161 ctcggcgcgg taaatagcgt gattcagcgc gcttcttcgc tggtatttga gagtatggaa
    17221 gccaaaaagc acgcgacgcg caatcgcgcc aatggcgagt tgttctatgg acggcgcgga
    17281 acgttaaccc atttttcctt acaacaagcg atgtgtgaac tggaaggtgg cgcaggctgc
    17341 gcgctatttc cctgcggggc ggcagcggtt gctaattcga ttcttgcttt tgtcgaacag
    17401 ggcgatcatg tgttgatgac caacaccgcc tatgaaccga gtcaggattt ctgtagcaaa
    17461 atcctcagca aactgggcgt aacgacatcg tggtttgatc cgctgattgg tgccgatatc
    17521 gttaagcatc tgcagccaaa cactaaaatc gtgtttctgg aatcgccggg ttccatcact
    17581 atggaagtcc acgacgttcc ggcgattgtt gccgccgtgc gcagtgtggt gccggatgcc
    17641 atcattatga tcgacaacac ctgggcagcc ggtgtgctgt ttaaggcgct ggattttggc
    17701 atcgatgttt ctattcaagc cgccaccaaa tatctggttg ggcattcaga tgcgatgatt
    17761 ggcactgccg tgtgcaatgc ccgttgctgg gagcagctac gggaaaatgc ctatctgatg
    17821 ggccagatgg tcgatgccga taccgcctat ataaccagcc gtggcctgcg cacattaggt
    17881 gtgcgtttgc gtcaacatca tgaaagcagt ctgaaagtgg ccgaatggct ggcagaacat
    17941 ccgcaagttg cgcgagttaa ccaccctgcc ctgcctggca gtaaaggaca cgaattctgg
    18001 aaacgagact ttacaggcag cagcgggcta ttttcctttg tgcttaagaa aaaactcaat
    18061 gatgaagagc tggcgaacta tctggataac ttcagtttat tcagcatggc ctactcgtgg
    18121 ggcgggtatg aatcgttgat cctggcaaat caaccagaac atatcgcagc cattcgccca
    18181 caaggcgaga tcgattttag cgggaccttg attcgcctgc atattggtct ggaagatgtc
    18241 gacgatctga ttgccgatct ggacgccggt tttgcgcgga ttgtataaca ttgccacttt
    18301 tggacaattt tgcagacatt ttattgtgaa aagtcttaaa ttgttgcgtc cgggatcaag
    18361 gcgtcccgga cgattcagga gtacaatagg cagataaagg cttaaacgct gttccacagg
    18421 aaagtccatg gctgttattc aagatatcat cgctgcgctc tggcaacacg actttgccgc
    18481 gctggcggat cctcatattg ttagcgttgt ttactttgtc atgtttgcca cgctgttttt
    18541 agaaaacggc ctgctgcccg cctcattttt gccaggcgac agcttgttga tattggctgg
    18601 cgcattgatt gcccaggggg ttatggattt tctgcctacg attgcgattc tgaccgccgc
    18661 agcaagtctg ggctgctggc taagttatat tcaggggcgc tggttaggga ataccaaaac
    18721 ggtgaaaggc tggctggcac agcttcctgc taaatatcac cagcgcgcca cctgcatgtt
    18781 tgaccgccac ggtctgctgg cgctgctggc tggacgtttt cttgcatttg tccgtacgct
    18841 gctgccaacc atggcgggaa tttccggtct gccaaaccgc cgtttccagt ttttcaactg
    18901 gttaagcgga ttgctgtggg tcagcgtggt aaccagtttc ggctatgcct taagtatgat
    18961 tccgttcgtt aaacgccatg aagatcaggt aatgacgttc ctgatgatcc tgccaattgc
    19021 cttgttaacc gctggcttgt taggcacgct gtttgtggtg attaaaaaaa aatactgtaa
    19081 cgcctgacga ttttccctgt tcccggttgc tgtaccggga acgtatttaa ttcccctgca
    19141 tcgcccgcat tcttgccgca tcttcccccg gcgtcacacc gaagtaacgt ttaaactcac
    19201 ggctaaattg cgatgcgctt tcatagccga cgcgcatcgc tgctgcgctg gccttcatgc
    19261 cgtcatggat gatcatcatc cgcgccttat gcagacggta attcttcaaa tactgcaacg
    19321 gcgaggtgct ggtgacagac ttaaaattat ggtggaacgc cgatacgctc atattagctt
    19381 ctgccgccag ttgctcgacg ctcaggtttt cggtgtattt attctcaatc cgtttcagca
    19441 cgcggctaat cagactgaag tgagtctggc gactgaccag cgccagtaac gcgccgccgc
    19501 aaggtccggt cagcacgtag tacagaattt cgcggatgat ctgtttgccg agaatacgcg
    19561 catccagtgg tcgcgccatc acgtcgagta accgctccgc cgcgcataaa atctcttctg
    19621 ataacgtggc ggagttaatc ccgctggctg ccatcgacgg ctggaaatgc tcatcttcgc
    19681 caatatccat caacagttcc tgtaactgca aaatatcgac attgagacgc aaccctgcca
    19741 gcggcacctc tgacgtggca taggtttcgc actcaaacgg caacggcacc gtcagcagca
    19801 ggtattcatt ggcatcataa cgaaacacgc gttcattgat ataaccgatt ttatgtccgg
    19861 aaaagagaat tatgatgcca ggctcgtaca tcaccggtgt acgtgcgaaa ggcgtctcgc
    19921 catacaacaa acgcacatcg ggcaacagtt ctgacaaact attttcttta tttttcagtt
    19981 tattaacttt atccgccagc aagcggcaaa tctcttcacg tttcatatcg cgtaatttct
    20041 taggaataat gcggcaattt gattgtgcgc aattttgtag catttctcca gcactctgga
    20101 gaaataggca agacattggc agaaatgagc attgagagcc agggcgctgg cgatcacaat
    20161 gaaaaacatc aggcagatcg ttctctgccc tcatattggc ccagcaaagg gagcaagtaa
    20221 tgaacaactt taatctgcac accccaaccc gcattctgtt tggtaaaggc gcaatcgctg
    20281 gtttacgcga acaaattcct cacgatgctc gcgtattgat tacctacggt ggcggcagcg
    20341 tgaaaaaaac cggcgttctc gatcaagttc tggatgccct gaaaggcatg gacgtgctgg
    20401 aatttggcgg tattgagcca aacccggctt atgaaacgct gatgaacgcc gtgaaactgg
    20461 ttcgcgaaca gaaagtgact ttcctgctgg gggttggcgg cggttctgta ctggacggca
    20521 ccaaatttat cgccgcagcg gctaactatc cggaaaatat cgatccgtgg cacattctgc
    20581 aaacgggcgg taaagagatt aaaagcgcca tcccgatggg ctgtgtgctg acgctgccag
    20641 caaccggttc agaatccaac gccggtgcgg tgatctcccg taaaaccaca ggcgataagc
    20701 aggcgttcca ttcagcccat gttcagccgg tatttgccgt gctcgatccg gtttatacct
    20761 acaccctgcc gccgcgtcag gtggctaacg gcgtagtgga cgcctttgta cacaccgtgg
    20821 aacagtatgt taccaaaccg gttgatgcca aaattcagga ccgtttcgca gaaggcattt
    20881 tgctgacgct gatcgaagat ggtccgaaag ccctgaaaga gccagaaaac tacgatgtgc
    20941 gcgccaacgt catgtgggcg gcgacgcagg cgctgaacgg tttgattggc gctggcgtac
    21001 cgcaggactg ggcaacgcat atgctgggcc acgaactgac tgcgatgcac ggtctggatc
    21061 acgcgcaaac actggctatc gtcctgcctg cactgtggaa tgaaaaacgc gataccaagc
    21121 gcgctaagct gctgcaatat gctgaacgcg tctggaacat cactgaaggt tccgacgatg
    21181 agcgtattga cgccgcgatt gccgcaaccc gcaatttctt tgagcaatta ggcgtgccta
    21241 cccacctctc cgactacggt ctggacggca gctccatccc ggctttgctg aaaaaactgg
    21301 aagagcacgg catgacccaa ctgggcgaaa atcatgacat tacgctggat gtcagccgcc
    21361 gtatatacga agccgcccgc taagcttttt acgcctcaaa ctttcgtttt cgggcatttc
    21421 gtccagactt aagttcacaa cacctcaccg gagcctgctc cggtgagttc atataaagga
    21481 ggaacgtatg gctaatccaa ccgttattaa gctacaggat ggcaatgtca tgccccagtt
    21541 gggactgggc gtctggcaag caagtaatga ggaagtaatc accgccattc aaaaagcgtt
    21601 agaagtgggt tatcgctcgt ttgataccgc cgcggcctat aagaatgaag aaggtgtcgg
    21661 caaagccctg aaaaatgcct cagtcaacag agaagaactg ttcatcacca ctaagctgtg
    21721 gaacgacgac cacaatcgcc cccgcgaagc cctgctcgac agcctgaaaa aactccagct
    21781 tgattatatc gacctctact taatgcactg gcccgttccc gctatcgacc attatgtcga
    21841 agcatggaaa ggcatgatcg aattgcaaaa agagggatta atcaaaagca tcggcgtgtg
    21901 caacttccag atccatcacc tgcaacgcct gattgatgaa actggcgtga cgcctgtgat
    21961 aaaccagatc gaacttcatc cgctgatgca acaacgccag ctacacgcct ggaacgcgac
    22021 acacaaaatc cagaccgaat cctggagccc attagcgcaa ggagggaaag gcgttttcga
    22081 tcagaaagtc attcgcgatc tggcagataa atacggcaaa accccggcgc agattgttat
    22141 ccgctggcat ctggatagcg gcctggtggt gatcccgaaa tcggtcacac cttcacgtat
    22201 tgccgaaaac tttgatgtct gggatttccg tctcgacaaa gacgaactcg gcgaaattgc
    22261 aaaactcgat cagggcaagc gtctcggtcc cgatcctgac cagttcggcg gctaacgtgc
    22321 aaattctccc ggtggcggta atgttccgct accggacttt tcagaaatca tttattcccc
    22381 tcgcgtcccg cccgttgtta ctcttccttg ttcaggaatg ccaaatataa ggacatcatc
    22441 atgcagagcc ggaagctctt aaaagaacaa ctcatctata tccgggataa acgcaacgga
    22501 gaggtgaaaa acagatgaaa ataatacttc tgtttttagc agccctggca agttttaccg
    22561 tgcacgcaca gcccccctca cagaccgtag aacaaacagt ccggcatatt tatcagaact
    22621 ataaatcaga tgccactgcc ccttattttg gtgaaaccgg agagcgtgcg ataacttctg
    22681 tgcgtattca acaggcgctt accctgaacg acaatcttac gctgccgggt aatattggct
    22741 ggattatgat ccggtttgtg attgtcagga ttttggcgat ctggtgctag aaagcgtagc
    22801 gataaccgaa actgacgccg atcatgccga tgccgttgtg cgctttcgta tctttaaaga
    22861 tgataaagaa aagaccacgc agacactgaa aatggtggcg gaaaatggtc gttgggtcat
    22921 tgacgatatt gtcagcaatc atggcagcgt cttacaagca gttaatagcg agaatgaaaa
    22981 aacgctggcc gctttagctt cgttgcaaaa agaacagccg gaagcctttg ttgccgaact
    23041 ctttgaacat attgccgatt atagctggcc gtggacgtgg gtggtttccg actcttaccg
    23101 ccaggcggtc aatgccttct ataaaaccac cttcaagacg gccaacaatc ccgatgaaga
    23161 tatgcaaata gaacggcaat ttatttacga caatccgatc tgttttggcg aagagtcgct
    23221 attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg cccgcattca
    23281 tattcgtttt acgctgacca atggcaacaa cgaagagcaa gaactggttt tacaacggcg
    23341 cgaaggtaag tgggaaatcg ctgattttat ccgcccgaac agcggcagcc tacttaagca
    23401 gattgaggca aaaaccgccg ccagattaaa gcaatgagct gaattaaata gcaattagcc
    23461 ggaacaataa aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct
    23521 ggcaacctgc gtgctcagtg gctgcgtcaa tgagcaaaag gtcaatcagc tggcgagcaa
    23581 tgtgcaaaca ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc
    23641 acaaatctat gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga
    23701 ctattttgat tgcctacgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca
    23761 gcatgtcagt tgccggggat tttttttaac gtccaaccgc cgctttaggg cgtttcttcg
    23821 caccagcatt caccggacga gattgcgtag acgacgcttt ttttgccgta gcaggcgtct
    23881 gacactgggt cgccatcggc gtatgtttcg tcaacgccgg acgggtattg cggttctggc
    23941 gacgagcctc acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctgc
    24001 caatcagatg ctttttgccc atcgcttcca gcgcctggcg gattaacggc cagtttgccg
    24061 gatcgtggta acgcaacaac gctttatgca aacgacgctg tttgtcgccc ttcggtacga
    24121 agacgtcttc actcttataa ccaatcttcg ccagcgggtt tttcccggtg taatacatgg
    24181 tggtcgagtt agccagtggc gatgggtaga agttctgtac ctggtcgaga cggaaacgat
    24241 gctttttcag ccacagcgcc aaattcacca tatcttcatc acgcgtaccg gggtgcgcgg
    24301 agatgaaata ggggatcaga tactgctctt tacctgcctg tttcgagtag gtatcgaaca
    24361 gctctttaaa gcggtcataa ctgcccatgc ccggcttcat catcttcgac aacggccctt
    24421 cttcggtatg ttccggagca atcttcagat aaccgccgac gtgatgggtc gccagttctt
    24481 tgatatagcg cggatcttcc acggcgatgt cataacgtac gcctgaggca atcagaatct
    24541 ttttaatgcc tttcagatcg cgagcgcggc gatagaggtt gatcgtcggt tcgtggttag
    24601 tgtccatatg cggacaaata tccgggtaaa cgcaggacaa acgacggcag gtttgttcag
    24661 cgcgtggcga tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa
    24721 ttacgcccgt aaaacctgga acggtgtcgc ggatcgcttc gatctcatta atgatcgaat
    24781 cttcggaacg gctctgaata atgcgccctt cgtgctcggt gatagaacag aaagagcagc
    24841 cgccaaagca gccgcgcata atgttgaccg aaaaacggat catttcgtaa gccggaatac
    24901 gggcattgcc ataggccgga tgtggcacgc gcttgtacgg cagcgcaaaa acgctgtcca
    24961 tctcttcggt agaaagcggg atagcaggcg ggttgatcca cacatagcgg tcaccgtgtt
    25021 tttgcatcaa tgcgcgggcg caacctgggt tagtttcgtg gtgcagaata cgcgaagcat
    25081 gggcgtacag cactttatcg cccttcactt tctcgaaaga aggcagcaac acgtaggttt
    25141 tttcccacgg tttcgggcgc ggtggctgca cggttacggc tttggcttcc tgctttttcg
    25201 gtgccaccgg tttgttatcc gcgcacggca aatcttcacc atacggatgc gggattgggt
    25261 cgatttttcc aggggtatca agacgggtgg aatccacgcc gctccagcca ggcagcgcct
    25321 ctttcacgat aatcgcggta ttacgcacat cgcggatttc actaatcggc tcgcccatcg
    25381 ccagacggtg cgccacctcc accagcggac gctcaccgtt accaaacatc agcatgtcgg
    25441 ctttcgaatc caccagcacg gaacggcgca cggtatcgga ccagtaatca taatgcgcgg
    25501 tacggcgcag actagcctca ataccaccga ggatcaccgg tacatctttc cacgcctctt
    25561 tacaacgctg ggtataaacc agtgtggcgc gatccgggcg cttacccgcg acgttatccg
    25621 gcgtgtaggc atcgtcatga cgtaaacggc gatcggcggt ataacggttg atcatcgaat
    25681 ccatgttgcc agcagtaaca ccgaaaaaca gattcggttt acccagacgc atgaaatcgt
    25741 ctttgctgct ccagtctggc tgggcgatga tcccgacgcg aaagccctgt gcttccagca
    25801 tacgaccgca aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca
    25861 aaatgatgtc gcagctatcc cagccaagtt gatccatctc ttcacgagac atcggcaaaa
    25921 acggtgccgg tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg
    25981 gttggatcag ggagatagag ctcattttgc ttccagaaat gataaaaaaa taatcaaagg
    26041 ccggggatta taagccggaa cgaaagagaa atcgaaaagt attccatact cgccctcctc
    26101 gggcgagtat gaagattacg gtaccggatt gactaacagt tgcccaatcg agccacggtc
    26161 cgccatttcc agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc
    26221 gaaataaaca agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc
    26281 ttccgggaac ggcatcgcac cattcacgtt acggatctgg aacatcacgc cttcaatatg
    26341 gaacgcttgc ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt
    26401 gacatcaata cggttgacgt cccacagttg tccattaata cccgggtcat cacccagact
    26461 gatatcgcga ctgcgaatcg gcgaaccggc catgatttca gttggcagca agcgcatcgg
    26521 aagactgtct gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccagggtaga
    26581 aaccagaatg ctggatggct caaagaagcc acgaatacga tcaacaatgc tcgccgcttc
    26641 gccacaggtg atcgacactt catcaccgtt gctcatatcc accagaatct cgcggcgctc
    26701 gcccggtgcc aacgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc
    26761 agaaatcaca tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga
    26821 cgcgttcagc agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac
    26881 accgttaacc agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg
    26941 cgtaccaaag ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata
    27001 atggttgggg ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag
    27061 gccgttatag acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc
    27121 tgcgttctgg cgaataggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc
    27181 cggaccgccc atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc
    27241 tgtcaggcgg ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg
    27301 ccccaggtaa cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca
    27361 gtgcgcacgt tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gcagcggcgg
    27421 aacgggtagc ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca
    27481 aagtgcaatc cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg
    27541 taaaactggc taaagtaata ccgtgcagga aagtgtttcc cgcaaaaata cagattgccg
    27601 acttaaactt ttccggcggc ttcgcgctct gcgacttctt tatcgagctc ggcgattttt
    27661 tgttccatta tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga
    27721 ctgacgtcaa ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga
    27781 ttaatcttat tcgaagttgt ggagacgcac acgggaataa tcgggacgcc cgccgcaatt
    27841 gccgcgtgaa atgccccagt cttgaacggt agcaggccgc gaccacggct gcgggtacct
    27901 tccgggaaca tccagatgga aatacggcgt tttttgaaat gattcactac ttccgcaata
    27961 gtgccgtgag ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac
    28021 aactgcccga agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt
    28081 tgcacaatgt tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata
    28141 gcattgccgt agctttcagc atctgcaggt ttgcggcact caactttcag gccgaacagc
    28201 ggcgcaagac gaccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg
    28261 aaaaggcagt aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata
    28321 agacgaaaga tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca
    28381 cctgaaatca ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg
    28441 caacgcatga ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg
    28501 ctgccggatg acgacttaac gtttcatccg gcgttccttg caagcgggag gaaacagcgc
    28561 cctccccggc atattactct tcgctatcac cgctgctggc acggcgagga gagtcgatct
    28621 caacacgatc gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag
    28681 tgactttctg tagctcttcc ggacgcagtt taattttgcg tttcccaaca tgaatggtca
    28741 gcgtgctttg cggcggcaga acgtacagtt gcgccagacc atcctcgcca cgcgcggctt
    28801 ctgccgatgg aatgttgata atcttgttgc ctttgccctt cgacagctgc ggcagatcac
    28861 ttaccgggaa catcaacata cggcctgcct gagtgattgc cagcagcata tcggaagcat
    28921 cttcaattac caccggcggc ataacatggg cattttccgg taaggtgatc aaagccttac
    28981 ctgcacggtt acgcgccacc agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg
    29041 aagccatcag cagtttctga tcgtcgcttt ccatcagcat atggtcaacg gtcgccccag
    29101 gcggcaacgt taatttgccg gtgagcggtt cgccctgacc acgcgccgac ggcagcgtaa
    29161 tcggatcaat ggcatagcta cgaccggtgg aatcaacaaa cactaccggt tggttgctct
    29221 tacctttcac cgccgctttg aagctatcac ccgctttata attcaggccc ggcgcgtcga
    29281 tatcatggcc tttagcgctg cgtacccagc ccatctgcga cagcacaatg gtgacaggtt
    29341 cagacggcag catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc tgcaacggcg
    29401 aacgacgatc gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc agcaggttat
    29461 tcattttacg ctcggaagcc aaaatgccct gcaactggtc gcgctctttt tccagctcac
    29521 tctgctcacc gcgaatcttc atctcttcca gtttggcaag atgacgcagt ttcagttcga
    29581 ggatcgcttc cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt
    29641 catcttcatt acgaatgatc tcaatcactt cgtcgatatt gagaaacgcc accagcaaac
    29701 cttcgaggat atgcaggcgc ttgaggactt tctccagacg atagttcagt cggcggcgca
    29761 cggtatcgcg gcggaacacc agccattcgg agagaatttc cagcaggttt ttcaccgccg
    29821 gacgaccatc cagaccgatc atattaaggt taatgcgata gctcttttcc agatcggtgg
    29881 tagcgaagag gtggttcatc acctgatcca tatccacgcg gttggaacgc ggcacaatca
    29941 ccagacgggt cgggttctcg tggtcagatt catcgcgcag atcgtcaacc atcggcagct
    30001 ttttgttgcg catttgcgca gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg
    30061 gcaatgcgct gataaccacc gcgccatctt ctttcttcca caccgcgcgc atacgcactg
    30121 aaccacgtcc gttctcgtag attttacgga tctcggcgcg cgaagtgata atttccgctt
    30181 cagtcggata atccggcccc tgcacgatat ccagcagctg atcgagcgtg gttttcggct
    30241 ggtcgattaa tgcgattgcc gcctgagcca cttcacgcag gttatgcggt ggaatatcgg
    30301 tcgccatgcc gacggcaata ccggtggtgc cgttaagcaa aatgtttggc agacgggcag
    30361 gtagcatttt cggctcctgc aaagtgccgt cgaagtttgg cacccagtca gccgtcccct
    30421 gccccaactc gctcaatagc agctcggaat atttcgacaa ccgggattcg gtgtaacgca
    30481 ttgccgcgaa cgatttcgga tcgtccggcg cgccccagtt cccctgacca tcaaccagcg
    30541 gataacggta agagaacggc tgcgccatca ggaccatcgc ttcataacag gcgctatcgc
    30601 cgtgcggatg gtatttaccc agtacgtcac cgacggtacg ggccgatttt ttaaatttgg
    30661 cgctggcatt caggcccagt tcagacatcg catacacaat gcggcgctga acaggtttca
    30721 gaccatcacc aataaacggc aacgcacggt ccatgatcac gtacatggag tagtttaagt
    30781 aggcgttttc cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc attaattctg
    30841 attcctcaac ttattcgcca gccttcaaag gggcaatatt gccggagata ctacattatc
    30901 tgccgcgtcg agtcacagga gatcagcgtg aatagcgccg ccggacgtgc tccgacggcg
    30961 ataaaggcat caatgtgcct tgatatacaa ctctttgctg tagacataat cctggggatt
    31021 atgcagcgga aaaccgccaa cgtagggttt aagcagtttg attaacggct gatagtagat
    31081 aggaatcagc ggtgcctgct ggttgacgat cacttctgcc tgctgataca acgcattacg
    31141 ctttgtcgca tcagtgatct gcatggcctg gtttagtaag gcgtcatact gcgcattttt
    31201 ccagtgaccg acgttttctt cactatcgct tttgagcgtg ttcaggaagc tggaagcatc
    31261 attgtacgtc gcatcccacg actgccgaga cagcatgaaa tcaccggctc gtcgggcatc
    31321 aagataggtt ttccactcca ttgtgcgcag cgtcacctgt gcacccagcc attttttcca
    31381 ttcggaagac aacgctatcg cggtcttttc atgcagatcg tacttgttgt agaacagctc
    31441 aaagcgtagc ggatgagagg cgtcgtatcc cgcctgtttc agcaaggctt ttgccatcgc
    31501 gacgcgctca ctcattggct tttgcagttc atcgaacgtc gtcgcgctaa agccttttac
    31561 ctctggcggc gtcagcgtgg ttgcgggcgt tctcaacccc agtacctttt gcgcaataag
    31621 ctgtcgatca accgtaagat atagcgcccg acgcactcgc acatcgttaa atggcggttt
    31681 ctcaaggttg aagttgtaat attcgctgtt cagacgcgga ataattcgta tctcgccagg
    31741 cagtgatttt tcaatggcgg gaatttgctg cgccggaacc caggtgagat cgacctctcc
    31801 cgcgcgatag cggttatagc cggtgaccga attatccagc gccagatact caacctgttg
    31861 caatactgta tgttgcgcat cgcggtactt tggatttttg cgtgcagtaa tcttttcgtt
    31921 aactacccac tgatcaagca caaaggcacc gttgtaaacc atgttctctg gcttactcca
    31981 gctatcgcca tgtttagcga tgacatgatg aggaaccggg aacagcgtcg gccaggcgag
    32041 catcgtcgtg aaccaaggaa ccggctgctc aagcgtaact tcaagagtac gatcatccgt
    32101 cgctttgaca cccagcgatg taacatctgc tttacccgca acaatagccg cggcattgtt
    32161 aatgtgcgcc tgtgccagat agccagcaaa agggcttgcc gttttcgggt caaccgcgcg
    32221 ctgccagccg aggacaaaat cctctgccgt cagaggctga ccgtcggacc actgcaaacc
    32281 gctacgcaga tggaaaatat agcgcctgcc gccgtccagt atctcccagc gttcagcctg
    32341 agcgggctgc acctggcctt caccgtccat ccataccaga ccttcaaaca gatccagcac
    32401 aatctgcgcg gcagtattct cctccacctt ttgcgggtcg agcgtacctg ggtcgctatg
    32461 attgttgtaa cgaaagactt gttgcggggc gagctgtgtg ttggtgggaa cgtcagcagc
    32521 gtagagagga gccgcactta ccaggctgac cagccataat aaatttcgcg tatacatggt
    32581 attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt gttcgctcaa
    32641 acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg ctaacggcac
    32701 ataaacattc gtttccagtc gctgctccgt caccccttcg gcaagattgg tgtagtgaaa
    32761 cagaatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc gaataatgcc
    32821 ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgagccacgg cataacgtcc
    32881 accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt cgctaacgct
    32941 gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt cccaggcgac
    33001 gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc acatgataaa
    33061 tttcgccacg ctgtagttga gcaactcagg cctacagcgg tgcggcaaca tcgccacagg
    33121 gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcatgt tgcctccctg
    33181 cgaaattatt cccgggaacg catcccggga gcggtaacaa ttacggattt actttgcgga
    33241 tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc tgaatctcaa
    33301 ccgtatcttt cggcgtcacc gtcacgccgt tccagcgttt gtggtcgata tcaacattga
    33361 tagtaccgct ggcatctttg aacacgtaga gatcgtcaga gatgcgttca acgatattgc
    33421 cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctaca gtcgttacgc
    33481 tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg ccagaaaaac
    33541 cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg attactgctg
    33601 cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt aacaagatag
    33661 tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag cgcgttacaa
    33721 cacggtttac tagcagcaaa tacggttatc gcagggatga aaaaatgcga attttactga
    33781 tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa atgggtttta
    33841 gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc gcaccttatg
    33901 atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt ttgcgcgaat
    33961 ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat gcgctggagg
    34021 aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct tttgcgttga
    34081 tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag gccagcaacg
    34141 agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg ctggctggcg
    34201 aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg cgtaacgctg
    34261 gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac gaagaggtca
    34321 ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt agtgatttta
    34381 ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta cccaacgtct
    34441 tagtctgcgt gtcaggctga cgctaatctt tttaattctg gcctcggtga cctggctgct
    34501 ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt tcgacaccca
    34561 actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca acgcggcgga
    34621 tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg atgcgctgac
    34681 ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata acggagaaga
    34741 tattccctat agctatcaac gggaaggttt tgctgacggg caactggtgg gcgataaaga
    34801 tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg ttgttggtca
    34861 ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat tgatcccgtg
    34921 gttggtcgca ctgccggtta tgttaatcat catgatggta ctactgggtc gtgaactcgc
    34981 gccgctgaac aaactggcgc tggcactacg tatgcgtgac cctgactcgg aaaaaccact
    35041 aaacgcgact ggcgtaccca gcgaagtgcg accactggtt gagtcgctaa atcaactgtt
    35101 cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg cagctcacga
    35161 acttcgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc tctctgacga
    35221 tgatccgcag gcgcggaaaa aagcactgct ccaattacat tccgggatcg atcgcgctac
    35281 tcgtctggtt gatcaactgc tcacgctatc gcggctggac tcactggata accttcagga
    35341 cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata tttaccacac
    35401 ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gtccagggca tcaaacgcac
    35461 cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg ccgtgcgtta
    35521 cagtccacag ggcagcgtgg tggacgtcac gctgaatgcc gataatttca tcgtaaggga
    35581 taacggcccc ggtgtgacac cagaggcact ggcgcgaatt ggcgaacgct tctatcgccc
    35641 acccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc gaatcgccaa
    35701 gctgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg aggcgaaggt
    35761 aagctggtaa gggtaagact tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt
    35821 tcaccgtacc gctctgtgac gtactatagt cggcaaacgt ctcaccttga ggttaaaaaa
    35881 tgagcaacat cctgattatc aacggcgcga aaaaattcgc ccactccaat ggtcaactga
    35941 acgacaccct gaccgaagtc gcggatggca cactgcgcga cctcgggcat gatgttcgca
    36001 tcgttcgcgc cgacagcgac tacgatgtca aagcggaagt acaaaacttt ctctgggctg
    36061 atgtggtgat ctggcagatg ccaggctggt ggatgggcgc gccgtggaca gtgaaaaaat
    36121 acattgatga tgtattcacc gaaggtcacg ggactctgta tgccagcgat ggtcgtaccc
    36181 gcaaagatcc gtcgaaaaaa tacggttccg gcggcctggt acagggcaaa aaatatatgc
    36241 tttctctgac ctggaacgcg ccaatggaag ccttcaccga aaaagatcag ttcttccacg
    36301 gcgttggcgt tgacggtgtg tatctgccgt tccataaagc aaaccaattc ctcggtatgg
    36361 aaccgctgcc gacatttatc gctaatgacg tgataaaaat gcctgatgtt ccccgctata
    36421 ctgaagaata tcgcaagcat cttgtggaaa tttttggtta actagagctc aggctttaga
    36481 aggagttaac catgcttacc gtaatcgcag aaatccgtac tcgtcctggt caacatcacc
    36541 gtcaggcggt attggatcag tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt
    36601 gccacggcta tgcgccaatg gtggattgcg ctgctggcgt gagtttccag tctatggcac
    36661 cggattctat cgtgatgatt gagcagtggg aaagcatcgc gcatcttgaa gcgcatctgc
    36721 aaaccccgca catgaaggcg tatagcgaag ccgtaaaagg tgacgtgctg gagatgaata
    36781 ttcgtattct gcagccaggg atttaacatt cctgaaaaga ggccggtgtt gctaccggcc
    36841 tcaggtcaga ttagccattc tttcttcccg ttttcagccc ccgatggaaa tcgttaattc
    36901 gcttaatggt tttaaccgca cgctgcggca ccgcggaagc caccgaccag acaatcattt
    36961 ccaggcacaa caataccgta ccatgtagtg gcgttttccc cttttcatcc ccgcgcggaa
    37021 catgaatcac gatactggcg tctttgctaa aacgtgaatc cagcgcgttt gtcagcaaaa
    37081 taacagggat ccccagccgt ctcgcttcac gcagcgttgt cagcccttcc cggtgcgcag
    37141 atttttgcgc catcatgatc agaacatcgc cacgttgaag cgcaatcagt tgctcggcaa
    37201 ggccaatacc cgtacggttt agtgccgtgg caggcaaacc tatgcggttg aatagtcgtg
    37261 cggtatagtc cgccagaata cccgaagcgc caatgccaaa aattgcgact tgccgtgcct
    37321 gtaccaacaa cgctaccgct tgcgcaagcg cgtggcggtt ttcaggctct gacaggacat
    37381 tacaggtata caaatgccct tcaagaacaa agtcgattgc ggtgttaaca tcgctcgtca
    37441 agttactcac cgtcgtagac atcttttcgc tggaactaag ggctggacca agccactgtt
    37501 ccagagtgcg tttcagatca cgcagcccgg caaaccccag ggcctgtata gccctgataa
    37561 ctgtggcatc ggaagtgttc aacgtggccg caatttccat cgccgtttga tcgagtacca
    37621 cttcacgatg ctcattaata tagctggcga cagctcttaa cccaggcgtt aactgactcg
    37681 cgcgagtgcg aaaacgctca ccataaatat caacccgcgt tttgtctttc tgaatcattt
    37741 ggcctccggt aaaggtcgcc ccgcaatttg tgattgtatc tgcgcagcca tcagccccag
    37801 agatgcaaag gagttagaaa ttgcctcttc acgagaaatc agcacataac gcccggaacg
    37861 acaggcagtc agaaactgac accatcccgg catgactttt tccattgctg ccagctcatc
    37921 ctgaggttta ccaccggtgt caccgcgcca ggttgcaaaa acaaaatcgg catccagttc
    37981 aggtaaacgc tccgcgctca catccattcg cccaccttca ggaatgctct caatcagcgg
    38041 cggaaagcga aaaccagcat cgcgtaatac ccgccccagc gaatgatagc tatgcatcac
    38101 attaattttt ccctgatttg cctgaatcac cgataccgtt attttttggc tgtccagcgt
    38161 cgccttcagc gcattgattt gtgcctgata gcggcgttca agtatcgcca gctgcgactg
    38221 cgttcctgtc agttctgcca gcttgcgata gatttctggc gcgccccctt tgagatgatc
    38281 aatgctcacc gtgggcgcaa ttttttccag tcgttcaatg ggtgtattac gggtcggttc
    38341 tgtgatgatc aggtcaggct ttgccgccac aatcgcttca atatcaatat cagcggtgcc
    38401 aataaaagcg atcgatgaat tatcaaagtc gacacctgtc aataacgcgc ctgatcgaat
    38461 gaaatggcta ccgtcaggcc gagttcgccc gtggctggcg acaggaggta caccgagctc
    38521 aatcagagga atagtgatat caagatcgtg taaagagaca attctctttg gatgagcagg
    38581 aacagtgact ttgcgcccta aatcatcagt aaagacctga acaggttctg tcgcgcgggc
    38641 aaagaatgac aacagtatca gcagagaaaa aaataaacgc atataactcc cattaaaaac
    38701 tgttcctgcg ctgccagagc agtagcaaaa agaatggtcc accaatcaat gaaataatga
    38761 ttccggcagg cagttgcaaa ggcaaaaaag ccaaacgacc aatgctatcc gccaggatga
    38821 ctaacaacgc ccccgatacg gcgctcccca gcagtaatgt cgtctgcccg ccacgtagaa
    38881 taaaacgtga tatatggggt gctatcaatc cgacaaaacc gatgttacca acacacgaaa
    38941 cgcagacggc tgtcaggatt attggtgcgg cgacacgtaa caacgccaga cgagaactat
    39001 tcacccctaa cccggttgca acctgatgac caagcagagc gatatccgcc gtcctggcgg
    39061 tgaacagcaa taaaagagcg gctggcaaca tccaacaggc tgaaataccg actaacatcc
    39121 agttagcagc atgcaggctt cccgataacc acatcaatgc agtttgtaca tctcgaacat
    39181 ctgcagtggt cataaataca ccgataccgg cggcgaaaaa ccaggaaaca ccaataccaa
    39241 tcagaacaaa tcgcgggcgg gaaatttcgc gtgcacaaaa aatcacgatt aaagccacca
    39301 acaagccacc agcaagacca gccactggac gccagaacat tcccagcatt ggaaactgga
    39361 aaatcagcca cagcaccgcc acgctacatc cttctttgac gccgattaaa ccaggatcgg
    39421 caaggccgtt acgcgcaatg gattgcatcg cggcccccgc catacctaac atggctccgc
    39481 atagcaatgc catgatgact ctcggcaagc gaatatcctg cacaatataa cgtgcgtctg
    39541 tgcttacgtt ttctggagca aacaacgctc gtcctacttc cgatgccggg acaaaaaaag
    39601 aaccatgcat cagaccgaat atcagtattc caaccgcaaa cagaaacagc ccaccaagca
    39661 acaccagatt tttagggcgt accagtgtcg aaaacttacc aacacgtagt ggacgtagcc
    39721 cgacgttcat tatccgactc atttgaacat tctcgtcgcc ataatgacaa aaacaggcgc
    39781 accaactaac gccgtcatga caccggtcgc taactcatgg ggcgtgaaaa gcgtccgagc
    39841 aataatatct gccagtaata acaaaagcgc gccgacacaa gctgaaagag ggagcagaac
    39901 gcgcaaatcc gctgatacca gacgcctgac aatctgcggt actagcagac cgacaaaacc
    39961 aatggggcca gcaatcgata cggctgcgcc acagagcaac gcgatcgcaa gcagagtgaa
    40021 tgttcgcgtg cggagtaccg agacgcccag cccctgcgcc atgcgatcac ccagtgccaa
    40081 catgttaaga gagggcgcaa ggtaaatggc taacacaacc ccccccagag aaaaccaggc
    40141 ggacgtaccc agcgtcgccc aatcctgccc cgctaaatca cctgctagcc atgtccgcat
    40201 cgccagcagt gtttgttcgt caagaatcag gattgcagcc gttattgagg atacgaacgc
    40261 cgatagcgcc acgccgcata aagtaacttt cattggcgtt agccctgaac gccctgcgga
    40321 ggaaagcagc aatatcaaca ggaacaataa cgcgccgccc gtagatgcca gcagtgggcg
    40381 tccaaccgga aaggccagcc caagcgcact ggcagcaacg acagcaagag cggctccggc
    40441 atttaagcct aaaatatgtg gctcccccaa tgggttacga atgacagcct gcaacaaagc
    40501 accagcaact ccaagagaag caccggtaag caatgctgcc gctaaacgcg gcaaacggag
    40561 tcttacaatt acgttgtgat cgaagttttg cggttcaaaa tgaagaaatg cctgcaacac
    40621 cgtttgtggt gcaataaatc gggcaccaat ccctggatat accacgcatc ccacaagcag
    40681 taaggcgagg aaaattacag taaccagccc tgtgcgcatc atactttttc tacaccacga
    40741 aacggcatga agaacggctt tcccgtcaga gtatttattg atgcgtgaac atcaacgtca
    40801 aagacagctt ttaccagctc aggcgtgcaa tgctccccct cattcaacac acgcaccact
    40861 ttcccctggc gtaaaaatat caacgtatca ccataattga cggcaaaatt cagatcatgc
    40921 aacaccacga cgactgtacg cccgtgatga cgcgttaaat catgtaataa ctcaagaatt
    40981 tcaacctgat aacgtaaatc aagccaggtt gtgggttcat ccagtaggat ataaggcgtc
    41041 ttttgcgcca acaccatcgc aatccagcaa cgctggcgct gcccaccaga gagtttttca
    41101 acaggtaaat gagcaaactc ctgcgtaccg gttaatttca gggcctcttc taccgcctgc
    41161 tcgtcggcgt cgctccattg acgtatgaaa ttttgccagg gaaaacgccc acgcgacacc
    41221 agttcataaa cggttaagcc ctccggcaat aatggcgact gcggcaagat cccaagtcta
    41281 cgggataacg ctttcgttgg ttgctcgtga atcgctttac catccagcaa tattgagccg
    41341 cccatgggtt gcaagacacg tgcaatggtg ctgagcaacg tcgactttcc acagccgttt
    41401 gccccaacca gaacggtcat tttttctgtg ggaacggaga aagaaacacc atcaacaatg
    41461 attttcttgt gatacccggc agaaacattg tcgaggatca gcccctgccc ttttgcgtta
    41521 tgtgccacgt gtacgccaac ttaatcaaag aaaaacaaaa ataaaactca ttctcttttg
    41581 agagtttgac gccacaaaat gtagtagtca agaagttaaa agccatacaa aaacaaggta
    41641 aataacaggt gaaatatttt taaagataaa aataaaatca ttaaatatta ataaattacc
    41701 atcaattgaa ttgcattaac ctctcataaa aacacatgta gcaatttttt attatttctc
    41761 atttactact acattaccgc atgattgaat gattctcaat tacatatccg atggtaaaat
    41821 aacaaacgga tagtgcttag tgagcaacaa gaaacaattt tcactcacga caatccgtga
    41881 gtttttggga taacagacta aatggctaag ttcacacctt cattctcagg aatcaaaggt
    41941 cgggcgctct tttcactgct ctttgcagca ccaatgattc atgcaaccga cactgcaacg
    42001 accaaagatg gcgaaacaat cactgttaca gcggatgcaa ataccgcaac tgaggcgacc
    42061 gatggttatc aacctctgag cacttccacg gcgacattaa ccgatatgcc gatgctggat
    42121 atcccgcagg tggtcaatac ggttagcgat caggttctgg aaaatcagaa tgcaacgacg
    42181 ctggatgaag cgctttataa cgtcagtaac gtggtacaga ccaatacatt aggcggaact
    42241 caggacgcct ttgtacgtcg tggttttggt gctaaccggg atggctccat catgaccaac
    42301 ggcctgcgaa ctgtacttcc tcgcagtttc aacgccgcca cagaacgtgt ggaagtgcta
    42361 aaaggtccgg cctccacgct gtatggcatt ctcgatcctg gtggattgat taacgtcgtg
    42421 accaagcgcc cggaaaaaac attccatggt tctgtctcag ccacgtcctc cagttttggc
    42481 ggaggcactg ggcaacttga tatcacaggt cccattgaag gcactcagct ggcataccgc
    42541 ctgacggggg aagtgcagga tgaagattac tggcgaaact tcggtaaaga gcgcagtaca
    42601 tttattgccc cgtcactcac ctggtttggt gataatgcaa cagtaaccat gctctattcc
    42661 catcgggact ataaaactcc gttcgatcgt ggaacgattt tcgaccttac gacgaaacag
    42721 cccgtaaacg ttgatcgaaa aatacgtttt gacgaaccgt ttaatattac agatggtcag
    42781 tccgatctgg cgcaactcaa cgcagaatat catctcaata gccagtggac agcgcgcttt
    42841 gattacagct acagccagga taaatacagc gacaatcagg ctcgcgttac cgcgtatgat
    42901 gcaacgacag gaacgctgac acggcgtgtt gatgcaactc agggatctac ccagcgtatg
    42961 cattctactc gtgcggatct gcaagggaat gttgatattg ctgggttcta taatgagatt
    43021 ctgggtgggg tgtcatatga atattatgat cttctgcgca cagatatgat tcgctgtaaa
    43081 aacgctaaag atttcaatat ctacaacccc gtttatggca ataccagcaa atgcacaacg
    43141 gtttcggcgt cggacagtga tcagacgatc aaacaggaga gctactcagc ttatgcacag
    43201 gatgcgctct atctgaccga taactggatt gccgtcgccg ggatccgcta tcagtattac
    43261 acgcagtatg cgggtaaagg ccgtcctttt aatgtcaata ctgacagccg cgatgaacaa
    43321 tggacgccca aactggggtt agtctacaaa ctgacgccat cggtatcctt atttgctaat
    43381 tattcgcaaa catttatgcc gcaatcgtca attgccagct acatcggaga tcttccaccg
    43441 gaatcatcta atgcttacga agtcggggca aaattcgagc tgttcgatgg tataaccgca
    43501 gatattgcgc tgtttgatat ccataaacgt aacgtgttgt ataccgaaag tattggtgat
    43561 gaaaccatcg ccaaaacggc aggccgcgtt cgttcaagag gggtagaagt cgaccttgcg
    43621 ggagcattaa ctgaaaacat taatatcatt gccagctacg gctataccga tgctaaggtt
    43681 ctggaagatc ctgattatgc agggaaacca ttgccgaatg ttcctcgtca taccggttcg
    43741 ctattcctga cctatgacat tcataacatg ccaggcaata acacactgac gtttggcggt
    43801 ggtggacatg gcgtaagccg tcgttcggca accaatgggg ctgactatta tctgccaggc
    43861 tatttcgttg ccgatgcctt cgccgcatac aaaatgaaat tgcagtatcc ggtcacactg
    43921 caattaaacg tcaaaaacct gtttgataaa acgtattaca cctcttccat cgccacaaat
    43981 aatctgggga atcagattgg cgatccgcgt gaagtgcaat tcacggtgaa aatggaattt
    44041 tgatcaaaaa agcccggtat acacttgtat accgggctat gttttattct tctggcggga
    44101 ggattatcag acctcaatct ccgccatgtc gcctttctct tgcaaccagt tgcggcgatc
    44161 ttccgagcgt ttcttcgcca gcagcatatc catcatcgcg tcagtacgct gatcgtcttc
    44221 atcatcgata gtcagctgca ccagacggcg agtgttcgga tcaagcgtgg tttcgcgcaa
    44281 ttgcatcggg ttcatttccc ccagaccttt aaaacgctgg acgttcggct tgcctttctt
    44341 gcgttttaat tgctcaagta cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc
    44401 tttcccgaga tcaatacggt agagcggtgg cagtgcgacg taaacgtgac cgtgtttcac
    44461 caacgcgcgg aagtgtttta cgaacaaagc gcagagcagt gtagcaatgt gcaaaccatc
    44521 agagtccgca tccgcgagga tacagatttt gccataacga agctggctca gatcgtcgct
    44581 gtcaggatcg ataccgatcg ctaccgaaat atcatgcact tcctgcgagg ccagcacttc
    44641 gtcggaagaa acttcccagg tgttaaggat cttacctttc agcggcatga tcgcctgata
    44701 ttcgcgatcg cgcgcctgct tggcagatcc gcctgcggag tcaccttcca cgaggaacag
    44761 ttcggtacgg ttaaggtcct gcgcggtaca atcagccagt ttgccaggca gcgccgggcc
    44821 gctggtcagc tttttacgca ccactttttt agccgcacgc atacgacgct gggcgctgga
    44881 aatcgccatc tctgccagca actccgccgc ctgaacgttc tggttcagcc acaggatgaa
    44941 ggcatctttc accacgcccg aaacgaatgc cgcacactga cgcgaagaga gacgctcttt
    45001 ggtctgcccg gcaaactgtg gatcctgcat ttttactgac agcacatagg cgcagcgatc
    45061 ccagatatct tccgccgaca actttacacc gcgcggcaaa atgttgcggt attcacagaa
    45121 ctcacgcatc gcgtccagca ggccctgacg caggccatta acatgggtac cgccctgcat
    45181 cgttgggatc aggttgacgt agctttcggt cagcagttca ccgccttccg gcagccacag
    45241 tagcgcccag tccaccgctt cagtatcgcc agcgaaatta ccgataaacg gtttttctgg
    45301 cagcgtcggt aaaccgttta ccgcttccgc caggtaatca ttcagaccgt cctgatagca
    45361 ccagcgctgt tcggtgttgt tgatctcatc tttaaaggta atctcaacgc cggggcacag
    45421 tactgctttg gctttcagca catgcgtcag gcgtgaaaca gaaaaacgcg ggctatcgaa
    45481 gaaggtttca tccggccaga aatggacgct ggtgccggta ttgcgtttac cgcaattgcc
    45541 gacaacctgt aaatcctgca ccttttcgcc attttcaaag gcgatgttat aaacctgacc
    45601 atcgcggcgc acgttaactt ctacgcgctt cgacagggcg ttaaccaccg aaatccccac
    45661 gccatgcagg ccgccagaga actggtaatt tttgttagag aatttaccgc ctgcatgcag
    45721 acggcaaaga atcagttcaa ccgccggtac accctcttcc gggtgaatat ccaccggcat
    45781 cccgcgccca tcgtcaataa cttctaacga ctggtcagca tgtaaaataa cgtccacgcg
    45841 ttttgcgtga cccgccagtg cttcatccac actgttatca atgacttctt gccccaaatg
    45901 gttagggcga gtggtatcgg tatacatccc cggacggcgg cgaaccggct caagcccggt
    45961 gagtacctca atggcatcag cgttataagt ttgcgtcatg gtttaagtta gcaattcgag
    46021 ttgatcgtca gagatggtgc agaccaagaa aatcgacgat cgggttgaaa taatcttcga
    46081 agcccgtgaa tgcgtggttg ccgccttcta tgacagtctg gcggcaggaa gcgtagtacg
    46141 ccaccgcctg gcggtaatcc agcacttcat ctcccgtctg ttgcagcagc cagatcaaat
    46201 ccggcgcttc cagcgggtca atctgcatga ctttaagatc gtaaatatgg cgtgactcta
    46261 gcacatattg ctgcccggtg taggggttct cgttctgacc gagatagtcc gtcagcagtt
    46321 caaacgggcg caccgccggg tttaccacca ctgcgggcag cataaaacat tgtgacaacc
    46381 aggtggcgta atatcccccc agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt
    46441 ccaggacaat ggattccagc agctctgccg cgtcggaagg atacggcggc aactgcggaa
    46501 tgatcatctc aacgtcaggg tgatgttccg ccagccagtt ttttaacaag ctcgcttttg
    46561 cagagcgcgg cgagctgttg aaaccgtgta aataaagaag cgtagacatc agtagccttc
    46621 tgaagcggta tcaggttgga aacgtgtgtc cgccaggcga tgcacctcgg tggtcagcgt
    46681 gccatcagca tgtaactcga gagtacgcca gccgggcgcg atagtatcca gcgtaaagtt
    46741 ggaacagtgc ggcttaaact gcacacaggt cgacggtgtt gccagcaggc ggcgaccgtt
    46801 ccagtcgaga tccagctcct gatgaatatg accgcacagc aagtatttga cgtgcggaaa
    46861 cttcgccagc acggtatcca gttcgcccgc gttacgcagg ctgtgttgat caagccaact
    46921 acaacccgca ggtagcggat gatgatgcag cagcagcaac gtatggcgct ctggcgcatc
    46981 agccagttta cgttccagcc actcaagctg aaactcgctt agctcaccgt gcggcacgcc
    47041 aaacacctgg ctatccagca acaggatttg ccattgctca ccaataaaca cccgcttcgc
    47101 cggggagata cccgcatcct gtaacgcgct gtacatcgcg ggctggaaat cgtggttgcc
    47161 cggcagccag acgcagggcg cacgaaaact tgcgatgcct tcagcgaaat gctgataggc
    47221 cgcagaggat tgatcctgcg ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg
    47281 gtgtggacga atcgcctcca gcaccgcctg gtagctttcc caggtgttta cccctaagag
    47341 ggcttcgtgc ttttgtgcaa acaggtgagt atcggtaatt tgtaaaatcc tgactctggc
    47401 ctcaccagcc agaggaaggg ttaacaggct ttccaaatgg tgtccttagg tttcacgacg
    47461 ctaataaacc ggaatcgcca tcgctccatg tgctaaacag tatcgcaacc agtccgctaa
    47521 aaactggtta atttgatgct tttcgtcgcg ttgatgcaac tttttattag gataatcata
    47581 ccgcgctttg aagcgaaaaa tctgctggct tgaacacact tcagccacca tcgcgtcatg
    47641 atacagacgc accgtcattg acggaaggct ccagtaactg atcgcgggcg cagtctgttc
    47701 tattgtcacc agggtagtgt atcgggtcga ttccacaatc gtcagccgat attgtgcgtt
    47761 tgccacctga tagcttacag tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa
    47821 ttgtgaaaag ttcatctcgc acaggcgcat catttcagga aagtcaggtg tgtaacgctt
    47881 catttatgcc cactcatttt ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat
    47941 gaccgacgct gcgttgtcga ttttcccctc ttctacccac tggtatgcct gttcccggct
    48001 taccacatga acgcgaatat cttcgttttc atcagccaga ccgtgaatac cgcttgcggt
    48061 cgtggcgtcc acttcgccca ccataattga cgaacgctca ctggtgcccc ccgggcttgc
    48121 caggaaactt aacaccggtt tggtccgttt gactatcagc cccgcctctt caatcgcttc
    48181 gcgacgggca acatcttcca cactttcacc ctcttcaatc attccggcaa ccatctccag
    48241 tagccaaggg gtttcgctag tgtcgtatgc ggcaattcga atctgctcaa tcagcacaac
    48301 ttcatcacgc actgggtcaa agggtagcaa gactgcggcg tgaccgcgct caaaaatttc
    48361 ccgccgtacc tcatgactca tttgcccgtt gaatagacga tgacgaaatc tataaagatc
    48421 taatgaaaaa aagccgcgat aaagtgtttc tcgtgcaata atttctacat cgtttttgcc
    48481 aaatgtaacg ggcaggttgt ctggcttaag cattgttaat gtcctggcac taatagtgaa
    48541 ttaaatgtga atttcagcga cgtttgactg ccgtttgagc agtcatgtgt taaattgagg
    48601 cacattaacg ccctatggca cgtaacgcca accttttgcg gtagcggctt ctgctagaat
    48661 ccgcaataat tttacagttt gatcgcgcta aatactgctt caccacaagg aatgcaaatg
    48721 aagaaattgc tccccattct tatcggcctg agcctttctg ggttcagttc gttgagccag
    48781 gccgagaacc tgatgcaagt ttatcagcaa gcacgcctta gtaacccgga attgcgtaag
    48841 tctgccgccg atcgtgatgc tgcctttgaa aaaattaatg aagcgcgcag tccattactg
    48901 ccacagctag gtttaggtgc agattacacc tatagcaacg gctaccgcga cgcgaacggc
    48961 atcaactcta acgcgaccag tgcgtccctg cagttaactc aatccatttt tgatatgtcg
    49021 aaatggcgtg cgttaacgct gcaggaaaaa gcagcaggga ttcaggacgt cacgtatcag
    49081 accgatcagc aaaccttgat cctcaacacc gcgaccgctt atttcaacgt gttgaatgct
    49141 attgacgttc tttcctatac acaggcgcaa aaagaagcga tctaccgtca attagatcaa
    49201 accacccaac gttttaacgt gggcctggta gcgatcaccg acgtgcagaa cgcccgcgcg
    49261 cagtacgata ccgtgctggc gaacgaagtg accgcacgta ataaccttga taacgcggta
    49321 gagcagctgc gccagatcac cggtaactac tatccggaac tggcggcgct gaatgtcgaa
    49381 aactttaaaa ccgacaaacc acagccggtt aacgcgctgc tgaaagaagc cgaaaaacgc
    49441 aacctgtcgc tgttacaggc acgcttgagc caggacctgg cgcgcgagca aattcgccag
    49501 gcgcaggatg gtcacttacc gactctggat ttaacggctt ctagcgggat ttctgacacc
    49561 tcttatagcg gttcgaaaac ccgtggtgcc gctggtaccc agtatgacga tagcaatatg
    49621 ggccagaaca aagttggcct gagcttctcg ctgccgattt atcagggcgg aatggttaac
    49681 tcgcaggtga aacaggcaca gtacaacttt gttggtgcca gcgagcaact ggaaagcgcg
    49741 catcgtagcg tcgtgcagac cgtacgttcc tctttcaaca acattaatgc ttctatcagt
    49801 agtattaacg cctacaaaca agccgtagtt tccgctcaaa gctcattaga cgcgatggaa
    49861 gcgggctact cggtcggtac gcgtaccatt gttgatgtgt tggatgcgac caccacgctg
    49921 tacaacgcca agcaagagct ggcgaatgcg cgttataact acctgattaa tcagctgaat
    49981 attaagtcag ccctgggtac gttgaacgag caggatctgc tggcactgaa caatgcgctg
    50041 agcaaaccgg tttccactaa tccggaaaac gttgccccgc aaacgccgga acagaatgct
    50101 attgctgatg gttatgcgcc tgatagcccg gcacccgtcg ttcagcaaac atccgcacgc
    50161 actaccacca gtaacggtca taaccctttc cgtaactgat gacgacgacg gggcttcggc
    50221 cccgtctgaa cgtaaggcaa cgtaaagata cgggttatct gccgcattct tcccccttct
    50281 cgcttcaatt tcgaccagcc atcctctatt ctgatgggta tttaccacag gtcccggaag
    50341 acaaaaatga aacggacaaa atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca
    50401 cgccatctga caccagtcgc tctcgcggtt gccactgttt ttatgctggc tggctgtgaa
    50461 aagagtgatg aaacagtgtc tctctatcaa aatgctgacg actgttcagc tgcaaaccca
    50521 ggcaaaagcg ccgaatgtac caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact
    50581 gcgccgaaat acgccacccg tgaagactgt gttgctgaat ttggtgaagg tcagtgccag
    50641 caggcaccag cccaggctgg catggcacca gaaaaccagg cgcaggccca gcaatccagc
    50701 gggagtttct ggatgccgct gatggccggt tacatgatgg gtcgtctgat gggcggcggt
    50761 gcgggatttg cacagcagcc gctgttctcc tcgaaaaacc cagccagccc ggcttacggt
    50821 aaatataccg acgcgacggg taaaaactat ggcgcagccc agccaggccg caccatgacc
    50881 gtaccgaaga cagccatggc accaaaaccg gcgaccacca ctaccgttac ccgtggcggt
    50941 tttggtgaat ctgttgccaa acaaagcact atgcagcgta gcgcaaccgg tacctcttct
    51001 cgttcaatgg gtggctgata ccgatggaaa gagtcagtat taccgagcgc ccggactggc
    51061 gtgagaaagc ccacgaatac ggtttcaatt ttcacaccat gtacggcgag ccgtactggt
    51121 gtgaagatgc ttactacaag ttgaccctcg cccaggttga aaagctggaa gaagtcaccg
    51181 ccgaactgca ccagatgtgc ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga
    51241 tgaccaaatt ccgcattcca aaacacacct ggagttttgt gcgccagtca tggctgacgc
    51301 accagccatc gctttattcg cgtcttgatc tggcgtggga tggcactggc gaacctaaac
    51361 ttctggaaaa taacgccgat acgccaacgt cactatacga ggcggcgttc tttcagtgga
    51421 tctggctgga agatcagctt aacgccggta acttgccgga gggcagcgac cagtttaaca
    51481 gtctgcaaga aaaactgatc gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt
    51541 tgctgcatct cacctgctgt cgcgacacgg tggaagatcg cggtaccatt cagtatttgc
    51601 aggactgcgc gacggaagct gaaatcgcta ccgagttcct ctacattgac gatatcgggt
    51661 taggtgaaaa aggtcagttc acggatttac aggatcaggt aatttccaac ctgttcaagc
    51721 tgtatccgtg ggaatttatg ttgcgtgaga tgttttccac caagctggag gatgcaggcg
    51781 tacgctggct ggaaccggcg tggaagagca ttatctccaa caaggcactt ctaccgctac
    51841 tgtgggagat gttcccgaat cacccgaacc tgctgcccgc ttattttgcg gaagatgatc
    51901 atccgcaaat ggaaaaatat gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt
    51961 cgatcattga gaacggcaaa accattgaag cagcggaagg tccgtatggc gaagaaggga
    52021 tgattgttca gcaattccac ccgttaccga aattcggcga cagctatatg ctgattggta
    52081 gctggctggt gaacgatcaa cccgccggaa ttggcattcg tgaagaccgt gcattgatca
    52141 cccaggatat gtctcggttt tatccacata tttttgttga ataatcaatg atgccggatg
    52201 gcattcgcca tccggtaatt gttaacctat ctgcaccgac agcatactta aactgcccat
    52261 ttctatacca tcgaccggaa tggtaattgg ctcctgccca tcccacgcac ctaagacata
    52321 cagcaacggc agatagtgct ctggcgttgg gttcgataac gcgccacctt catggtcgag
    52381 ataattcacc agaggatgct gctctaccgg cccttgccac gtcagattcg ctttcacata
    52441 ctcattaaac gacatcgccc aaggatacgg tgaactatca ccgtgccact tcactgtacg
    52501 caggttatgc accacgttgc cgctggcgac caacattatt ccttcatcac gcagcgccgc
    52561 gagttttcgc cccatttcga aatgccaggc ggcaggtttg ctactgtcga tactcaactg
    52621 caccatcggg atatccgcgt caggatacat cttaatcagc acgccccagg aaccgtggtc
    52681 aaagccccag gcttctttat ccagcgtcac cgggatcggc gctaacagct caaccagacg
    52741 ctgtgccagc gcaggcgaac ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa
    52801 gccaccaaag tcatgaatcg tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt
    52861 aaaccagtga gccgaaacta ccacaatcgc ttgcgggcgt ggcaatgtca tccccaacgt
    52921 ctgccagctg cgggtataca aattatcttc cagcacgttc atcggactac cgtgacctaa
    52981 aaacaatgct ggcatacgtg ttgaagacat gatgatatcc ttaactaaag gtgtcatttt
    53041 gatatcctca cgatacgctt gttcggcgga gtaagaaccc ggataacaat gatgatgatc
    53101 atcagttatt ttgacgatct gcctgaaggt gaagatttat aaggagttgt cgatgtcagt
    53161 acctctcatt ctgaccatac tggcgggggc agccacgttt attggcgcgt ttctcggcgt
    53221 tctcgggcaa aaaccctcga accgcttact ggcgttttcg ctaggatttg cggcggggat
    53281 catgttgctc atctcattaa tggaaatgct tcctgccgca ctagcagctg aaggaatgtc
    53341 gcctgtgttg ggttatggaa tgtttatctt cggtctgctt ggctattttg gcctggaccg
    53401 catgttgccg catgcgcatc cgcaggattt aatgcaaaaa tcggtgcagc cgttgccaaa
    53461 atcgatcaag cgcacagcca ttctgctcac tctcggcatc agtctgcata acttcccgga
    53521 agggattgcc acctttgtca cggcgagcag caacctggag ctgggatttg gcatcgcact
    53581 ggccgtcgcg ttgcacaata ttcctgaagg tctggcggtt gcaggcccgg tttatgcagc
    53641 aacgggttct aaacgcacgg caattctgtg ggcggggatt tctggactgg cagaaattct
    53701 tggtggtgtg ctggcgtggt taatcctcgg tagcatgatt tccccggtgg tcatggcagc
    53761 aatcatggcg gcggtcgcag gaattatggt ggcgctctcg gttgatgaat taatgccgct
    53821 cgccaaagag attgacccta ataataaccc cagctatggc gtactatgtg gaatgtcagt
    53881 gatgggattc agtttggtgc tgctacaaac ggcgggaatt ggttaaaagc aacatcagaa
    53941 gttgtccagc gcagggacaa cttctgctag tttcactaaa gccttattct accttttcgg
    54001 ctgtctcttc tctcgtactg tttaagtatt tgttccgcgc ttcaagcagt gcgtctctgt
    54061 cctggcgaaa tgcccgacaa taacgtttca aatgcaggaa ataacccacc acaaccagaa
    54121 caatcaatac aatccagtac caggcgataa acatcctttt acccttaata tttaacaata
    54181 ctaaacattt agcgcataaa tttcacatat cctttttcgg atatattcat atggtcgcat
    54241 aataacggaa caattatatg attttgatca taatgaaaaa gaatgcctga tattaatgaa
    54301 attttctcat agaaagagaa gcggaattaa catcttgcat agtagggaaa aataaaaacc
    54361 gaagcctgct acaacttcgg tttcttgatt aaggcagtaa attaagcagc ggttttcagc
    54421 tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca
    54481 tgttgtgttt attggcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat
    54541 tagtcagctc acacagcaca ccagccggtt tgaagcctgc cagcgtcatt agatcaatag
    54601 ttgcttcagt atgaccgcca cgcgtcagta caccaccagc ctgagcgcgg agtgggaaaa
    54661 cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg
    54721 tcgtaatacg gtcagcggca gaaacaccgg tagtcacacc ttcagccgct tcaatggtca
    54781 cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc agatcgagtt
    54841 gtttacggcg atcttcagta atgcacaggc aaacaatacc actaccgtgg cgaatggtca
    54901 gcgccatctg ctcaacagtc atggtttctg ccgggaagat catatcacct tcgttttcac
    54961 ggtcttcatc atcaagcacc attacaccgc gtccttcacg cagcgcagcc agtgcatttt
    55021 caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaac
    55081 ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa
    55141 aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac
    55201 cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc
    55261 gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag
    55321 cggattcact ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt
    55381 ttatgactta cccttatcgc actacaatgg cactcaacac ctatcagtac agggaaacta
    55441 caatgattga cccgaaaaaa attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta
    55501 aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagcgc
    55561 agctgacgcg tctcgatctg gtaagccgcg aagaattcga cgttcagacg caagtcctgt
    55621 tgcggactcg tgaaaaactg gcgttactgg agcagcgcag cagtgaactg gaagcgcgca
    55681 ataattcggt agcagatctt caatctcctc cggctatccc gcctattgat aaagcagaat
    55741 aaagcaacac aaaaacgggc cagacggccc gtaatatcag tgttctaatt caagttctct
    55801 ggtttgcatc atctgttggc aataccaggc ataacgtgcg caaaaccagg ttctatgttc
    55861 ttcatccatg ttaccagtaa cggccagaat atcgaccggg cgaccttctg cgtgcattct
    55921 ggcaaaacta cgcgctagga aatcgaaatt atttaaagaa ttaagactat gatccataag
    55981 cacatcctcc ggttatcgca taccttttaa ggccatatgc tttttctgat ttcagtattc
    56041 cccaatcagt gtagtgcctt taaacataac gtgcgtaaat atattgtccc cgatcacact
    56101 ttttagtgaa aaataaaaaa ccatattaaa agactttctc ttccagaacc tctctaacat
    56161 atgttattat taacaaagac aaatgtaagc aaggaaacta tcacaaagta aatattgcgt
    56221 cactaaatgg acattggagt gacatatgat tttattcgcc gactataaca ccccttatct
    56281 tttcgctatt tcctttgtac tgttgatcgg ccttttagaa atttttgcgc ttatctgcgg
    56341 tcacatgctc tccggtgctc ttgatgcaca tcttgaccat tatgattcca taaccacagg
    56401 tcatattagc caggcacttc attatctcaa tatcggaaga ttgccagctc tcgtcgttct
    56461 ttgcttactg gctggttttt ttggtcttat tggcatcctg ttgcagcata cctgcatcat
    56521 ggtctggcaa tcaccactct cgaacctgtt tgtggttcct gtcagtttac tatttacgat
    56581 tattgcggtg cattataccg gtaaaatagt tgcgccctgg attccacgcg atcatagctc
    56641 agctattaca gaagaagaat atattggtag catggcgtta attaccggtc atcaagccac
    56701 ttcaggtaat ccctgcgaag gaaaactcac cgatcatttt ggccaaatac attatttact
    56761 actggagcct gaagaaggaa aaattttcac taaaggagac aaagtgttaa ttatttgccg
    56821 actttctgca acgcgatatt tggcggaaaa caatccctag ccccaaatat tgtaatttat
    56881 taatcaaaag gaaatttaat aatggatgat attgttaatt ccgtgccctc ctggatgttt
    56941 accgcgatta ttgccgtatg cattctgttt attattggaa ttattttcgc caggctctat
    57001 cgtcgcgctt cggcagagca agcatttgtc cgtaccggct taggggggca aaaagtggta
    57061 atgagcggtg gcgcaatcgt gatgccgatc tttcatgaaa taatccccat caatatgaat
    57121 actctgaagc tggaagtcag ccgctcaacc attgatagcc tgattacgaa agatcgtatg
    57181 cgcgtcgatg tagtagtcgc tttctttgtg cgggtaaaac cttcagtaga agggattgcc
    57241 accgctgccc agacgctggg gcaacgcacc ctgtcgcctg aagacttacg tatgttggtt
    57301 gaagataaat ttgtcgatgc cctccgtgca acagctgcgc aaatgaccat gcatgagtta
    57361 caggataccc gcgagaactt cgtgcaggga gtacaaaata ccgtagctga agatctgtcg
    57421 aaaaacggcc tggaactgga aagcgtttca cttaccaact ttaaccagac ctcgaaagaa
    57481 catttcaatc ctaacaatgc ctttgacgcc gaaggtttaa ccaaactgac tcaggagacg
    57541 gagcgccgtc gccgcgagcg taacgaagtt gaacaggatg tagaagttgc ggtgcgtgag
    57601 aaaaaccgtg atgcactttc gcgcaagttg aagattgaac aacaagaagc gtttatgacg
    57661 cttgagcagg agcagcaggt taaaacccgt accgctgagc agaatgcgaa aattgcggct
    57721 tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa ttctggctga acgacagatt
    57781 caggaaacag aaatcgaccg cgaacaggcc gtccgctcaa gaaaagttga agctgaacgt
    57841 gaagttcgca ttaaagagat cgaacagcag caggtcaccg aaatcgctaa ccagacgaaa
    57901 tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg cagaagcgcg tgctaacctt
    57961 gcactcgcag aagcagtaag cgcccaacaa aacgtagaaa ccactcgcca gacagccgaa
    58021 gccgatcgtg ccaagcaggt ggcgctgatt gccgcagcac aggatgcaga aaccaaagcg
    58081 gttgaactga ccgtgcgggc gaaagcagaa aaagaagccg cagagatgca ggcggcggcg
    58141 atcgttgagt tagccgaagc tacgcgtaaa aaaggcctgg cggaagcaga agcacaacgt
    58201 gcgctgaacg atgctatcaa cgtactttct gacgaacaaa ccagccttaa attcaaactg
    58261 gccttgttgc aggcgctacc tgcggtaata gagaaatccg tggaaccaat gaagtccatt
    58321 gacggtatca agattattca ggtcgatggt ctgaatcgtg gtggtactgc gggggatgca
    58381 aacacgggta atgtgggggg cggaaacctg gcggaacaag cattatcagc cgcactctct
    58441 taccgcacac aggcaccact gattgactcc ttgctcaatg aaattggcgt ttcaggcggc
    58501 tcactggctg cattgacttc actcttaacc ccaacaactc ccgtcgccga aaacgtagaa
    58561 taaattttca gctatgaaca tccctggctc aaaaagccgg ggatttttta tatctgcgtt
    58621 ccgctaaaag gtgcaaatga tcaggctgtt gcagcgtttt gcgtgattgc tcggggaagg
    58681 caaaactgct tcagggaaag cgttgcgcgg ggtccggcgc tcatcaacaa tcggggagta
    58741 agcaaagggc tgaaacggga aagcccctcc cgaggaaggg gccttacaga aggaaagggt
    58801 tatgatgaag ctcgtcatca tactgattgt gttgttactc gtaagtttcg cagcttatta
    58861 acagccaatc agagggggga gaaatcctcc ctcacccttg ttcctttact ttaggttgaa
    58921 aaaaaatcag cgtcaagaag gtttcattaa cacatatccc tggcccaaaa agccggggat
    58981 tttttatatc tgcgttccgc taaaaggtgc aaatgctcag gacgttgcag cgttttgcgt
    59041 gaccgctcgg ggaaggcaaa actgcttcag ggaaagcgtt gcgcggggtc cggcgctcat
    59101 caacaatcgg ggagtaagca aagggctgaa acgggaaagc ccctcccgag gaaggggcct
    59161 tatagaagga aagggttatg atgaagctcg tcatcatact ggttgtgttg ttactgttaa
    59221 gtttcccgac ttactaacaa ctcatcagag gggggagaaa tcctccctta cccttgttcc
    59281 tttactctag gttgaaaaga caacagcgtc aataggcctg ccatgtacga agcgagatct
    59341 gtgaaccgct ttccggttag ccttttttat cctgttggat cttcttgata atgttagtgg
    59401 tcgagcaacc gtcttcaaag ttgagcacca acacttcgcc gccgttggcc cagacttctt
    59461 tactcccggc gatctcttcc ggtttgtagt caccgccttt caccagcagg tctggcagga
    59521 tcccggcgat cagacgctgc ggcgtgtctt cttcaaacga cactacccag tcgaccgctt
    59581 ccagtgcgcc cagcacaatc atacgctgtt cgagtgggtt taccgggcgg gaatcccctt
    59641 tcagccgttt ggtggaggca tcgctgttga cggcaacaat caagcggtca cccagcttgc
    59701 gggcatttgc cagataagag acgtgcccgg catgtaggat atcgaagaca ccgttggtca
    59761 ttaccacttt ttcaccacgt ttacgcgctg ccactacagc cagcttcagt tcctcttcgg
    59821 tcatcacgcc aaagccggta tctgcgcgtc cacgtacagc gttttccagc tcgatcggcg
    59881 aaaccgtgga cgtccccagt ttgccgacca ccacaccagc cgccgcattg gcaaagaagc
    59941 aggcttcttc cagcgaatta cccgctgcca gcgttgccgc caggacgcca atcaccgtgt
    60001 cgcccgcacc ggtaacgtca tacacttcct gcgcctgagt tggcatatgc agcggcgctt
    60061 tacccggttg cagcagcgac ataccctgtt cggaacgggt cactaacaga gccgagagtt
    60121 cgtaatcggc aatcagtttc atgccgcgct caacaatctc ttcttcggtc ttacatttac
    60181 cgacaacagc ttcaaattcc gagagattcg gcgttaacag cgtagcgccg cggtagcgct
    60241 caaaatcggt accttttgga tcaatcagca ccggaacacc cgctttacgc gccagttgga
    60301 tcatctgctg tacgcttgcc agcgcacctt tggcgtagtc agaaagcacc agcgcgccaa
    60361 tcgaactcag cgcctgatta atccgctcgt gcagcggctg cggatcaaca ccttcgaaac
    60421 cttcttcaaa atccagacgg atcagctgtt ggttgcggga aagtacccgt aatttggtaa
    60481 tggtcggatg cgtcggtaca gaaacgaagt cgcatttgac gttgacgtcg gccagagatt
    60541 tactcagcgc gcgcgctgca tcgtcaatgc ccgtcaaccc gaccaggcgt gcattagcac
    60601 cgagagaagc gatattcatc gccacgttag ccgcgccgcc cggacgttct tcgatggtat
    60661 tcactttaac cacgggcacc ggcgcttccg gcgagatacg actggagggg ccgtaccagt
    60721 aacgatccag catcacatca ccaaccacca tcactcctgc acgttcaaac tctggcagcg
    60781 ttactttcat tcctgtctcc tgagagattc aaaatttgcg cgcgataata ccatacttca
    60841 ttcttccacc agccacttct gccagcttgc ccgcaccagt tcacgctctg cggtgaagca
    60901 atcctccggc acatggcccg gcaattcctg taatgccaga tgatgaagtt catcgcgaag
    60961 cgtagtgtaa gcacgggtca gcgccatcgc ttcctgctct tccataatgt cgttttgcgc
    61021 cagtagttcc agaatgcgca cgttgtctga ccagcgcgtt aacttcggtt tttcatgagc
    61081 gtagcgcaac accagatatt gggtaataaa ttcgatatcg gtaattcccc cttcatcagc
    61141 tttgatatca aagcgatcgc gatgtttatt gccgagatga gcgcgcattt tctcgcgcat
    61201 ttcccgcact tccgtttgca gagttttacc ttcacgcggc agcgtcataa tctcgcgacg
    61261 cactgcgtca aagtgcgcgg tgagctgcgg atcgccgtac actacacgcg cacgcaccag
    61321 cgcctgatgc tcccacgtcc aggcctcgtt tttctgataa tcggcaaatg cttctgtgga
    61381 ggtaaccagc attcccgccg ccccggacgg gcgcagccgc gcatccactt cgtacaaaat
    61441 gccggaagag gttcgcgtgc tgaacaggtg cataatgcgt tgcgccagac gcagataaaa
    61501 ctgtcgtccg tcgatttccc gctcgccgtc agtcatcgca tccattgggc aatcatggag
    61561 gaagatcagg tcaagatcgg aactgtagcc taactcccag ccgcccagct tgccgtagcc
    61621 gaccactgca aaaccacgcc cttcgcgttc gttcaggtga ttcggcttac cgtagcgggc
    61681 aaccatttga acccacgcct gctgaacgac ggcatctatc atggcttccg ccagccaggt
    61741 taagtgatcg ctcactttca tcaccggtag cgtaccggcg atatccgctg cggcgatgcg
    61801 taacagctgc gcctgtttga actgacgcag cgcctcaagc tgttgctctt cgtcatcttc
    61861 cggcacgcgc agcaaatact ggcgcaactc atcgcggtag gcatcggtcg ccgtcggctg
    61921 gtaaagggtg tttggatcga gcaattcatc cagcaataat ggataacgcg ccagctggct
    61981 ggcaatcatc ggcgacgctg cacacagaga aatcaaatgt ttaagcgccg cggggaattc
    62041 actgagcaat tctaaatagg tggtgcgggt aacaatcccc gccagcaagg cggtaatgcg
    62101 caacagcgta acggcagcgt cttcacgcgc acagacatca cttagcagat gcggcatcag
    62161 atggtcgagc acctgacgcc cgcgtggccc gattgtgcgc ttatccagct ctttgcggaa
    62221 atcggcaatc agcgtcagca cctgtttgcg atcgtcctcg ctaagatgcg ccagcactgg
    62281 tgtagtgtca tcttcctgca acgcatcctg ccacagctcg cgccactgtt ccgacagcga
    62341 ctcttcctga gtttcacttt cgtcgtcgcc aatcaattca ttaaacaccc ggcgcacatt
    62401 ggtcatatgt gcggtcagcg ccccggtcaa ttgcggccag tcagcaaagt ccatcgccca
    62461 cgccagccgc gcacggttaa gctcatcaga aggaagcgtc tgggtttgtt cgtcgttaat
    62521 gctttgcagc aggttttcca gacgccgcag gaacagatac gccactcgca attgttcagc
    62581 atcgttttca gaaagcagat gcagcgcggc aatgacgctg agcgttggca gtaaagagcg
    62641 cgactgcagc gacggttcgc gcccgccgcg aatgagctgg aacacctgaa cgataaattc
    62701 aatttcgcga atgccgcctg cgccgagttt gatattgtcg gtcaaaccac gtcgacgcac
    62761 ttcacgggca atcattcctt tcatgttgcg cagcgactga atcacgctga aatcgatgta
    62821 acgacggaaa acaaacgggc gcagcatcgc acgcaactcg ttagcataga cgccttcgct
    62881 atcgcccata atccgcgcct tgaccatcgc gtaacgctcc cagtcgcgcc cctgctcctg
    62941 gtaataatct tccaacgcgg caaagctcag caccagcggg ccactttcgc caaacggacg
    63001 cagccgcata tccacgcgat agacgaagcc atcctgcgtt ggttgatcca gcactttaat
    63061 cagccgctgc cccatgcggg taaaaaactg cgcgttatcc agttcccagc gtccaccctg
    63121 cgtacaacca tgttccggcc aggcaaaaat cagatcgata tcagaggaga aattcagttc
    63181 cccgccgccc agcttaccca tgcctaaaat cagcagcggt tgcgcttcgc cctgcgcatt
    63241 gcacggcgtt ccccactcgc ggcagcaggc gtcatatagc cagtcacgcg ccgcaacaat
    63301 cagcgtctcc gccagatagc tgagctgctg caatatgctc tcttcagtaa ccagtgccag
    63361 cgtttgcgcc caggcgatac gtaccatgat gcgccgacgg aacagccgca gctcgcgcat
    63421 taacccggct tcgtcactca cattaatgag cgcctcctgc aaccatgcaa cgtaatgctg
    63481 ccattcgtcg gcctgcggcg gttggctttc cagttccgtc agccactctg gatgcgcact
    63541 tatgctgtcc tgcacaaaat cactgaaagt aagtactgac ttcgcctgtg cactaagtga
    63601 ttcctcggct aaaggctctg gcagccgctc aacaatggtc tgccagtact gctgtaacgg
    63661 tgaagagagc ggcttcataa gcgattttat ccttgcctga ttaacgtttt ccgctgtgca
    63721 accagaacgg ttcctgattg tttgcctcat tacggaaatg ttcaatttcg atgcgttgcc
    63781 cggtcgcaat agcgtgatgc agcccctgcc agttctccag ccaggcttgc gcgacgacag
    63841 gatcatagta acccgccagc aacagtattg agtcaatatc acgcgtcagg cgtggcaact
    63901 ggtcacggta gcgatcgcct aacggctggc agaaaacgct tttcagttca gcggcatggc
    63961 gggaaagatg gatatcggca aagcgtttga aggagtcgct gattttgccc tgcgctttgg
    64021 catctaaaaa tggctgccat gctttgctta ccaaccattc ggtcaacgcc agcttcgcca
    64081 ttgccgtttc ggtagagtag accgccgtca cggcagaaac cgcagaagca atggtcgctt
    64141 cgcattgggt cagcaaatca cgtaagtgag tgctcgcttt acgcggcaca ataccaccga
    64201 acagcatcag cgtatgacgg accaagctaa tggctgccag cacctgttct ttcgccgcat
    64261 cgttgccgcg tacccacagt tcttcatgat actgccattg cgctaacgcc agctcgagcg
    64321 ccgcttccag cccctgttcc acatcggctt ttgccgcaac atgcaaaatg gtggtcggtt
    64381 tgatttcacg cgccggattg ccctgcgcca gatgatagcc acgcgccgct ttgctcaggc
    64441 tgccctggcg taatccggtt tgcgatacca gttggttcgc cagtttcagc accgcgcgcg
    64501 tgtcgccgct aagcagctcc agttccagct cacagatagg ttcagcaaat tcccccgctt
    64561 tcacctcacc ctgatcgaga gcgatttcaa ttcggctgcc atcgaccgcc accagccatt
    64621 tttcgcgata aaaatcggtg ctgaacagcg gttgtacgcg ggaagcgaga tcggcgggca
    64681 attcgccgtt cggccagact tccgtcggta actgcgccag gtcgagcgtc ggttcgctca
    64741 acgccacgtt atattccggg cgctggtgta agccgcctgt cactcttcct gcaactttca
    64801 tggtcatctc atagcgaccg ttttcgccac gaatacgtaa gcccatatcg tgcccacgca
    64861 gccagttatc cggcgtttcg tagtaaatat tcagtaactg cacggggtca tggtgctcgc
    64921 cgcccagcgt attgagatgg tcacgcaacg cctcaacggc gctgtgatta acaataaact
    64981 ttaattcgat ttcctgagcc atggccttta cttatcggtt atgtcacata agtgacgatg
    65041 aacggcgaac ttaatgcgat cttttttgtc agtagatagt attttgcgcc aaattgccat
    65101 gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg gcaagacctg acacagagga
    65161 ttccgattga tgtcgaatgc tttgcgtagc gacactgatg ccactactat cgttccactt
    65221 tccatgacaa taacgacagc ctgatgccaa aattacgcct gatcggatta actttactcg
    65281 cacttagcgc gactgccgtc tcacacgctg aagaaacgcg ctatgtttct gacgaactga
    65341 atacctgggt ccgtagcggt ccgggagatc attatcgcct cgtgggcacg gttaacgccg
    65401 gcgaggaagt gaccttatta caaactgacg ccaacaccaa ttatgcccag gtgaaagaca
    65461 gctctggccg taccgcctgg atcccgttga aacaacttag cactgagcca agcctgcgct
    65521 cccgtgtgcc agatctggaa aatcaggtca aaaccctgac cgataaactc accaatatcg
    65581 ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa agtggcgcag agcgacagcg
    65641 tgatcaacgg gttaaaagaa gaaaatcaga aactgaaaaa cgagctgatt gtcgcgcaga
    65701 aaaaggtcga tgccgccagc gtgcagctgg atgacaaaca gcgcaccatc atcatgcaat
    65761 ggtttatgta tggcggcggc gtgctggggc ttggcttgct gctcggtctg gtactgccgc
    65821 acctgatccc aagccgcaaa cgcaaagatc gctggatgaa ctaaatcgcc ttcttcgttg
    65881 cactgtctta ttattaggaa ttgtgtattt gacgaaggga tgatggcgtg aagatttatc
    65941 tggtcggtgg tgctgttcgg gatgcattgt tagggctacc ggtcaaagac agagattggg
    66001 tagtggtcgg cagtacgcca caggagatgc tcgacgcggg ctaccagcag gtaggccgcg
    66061 attttcctgt gtttctgcat ccgcaaacgc atgaagagta tgcgctggca cgtaccgaac
    66121 ggaaatccgg ctccggttac accggtttta cctgctatgc cgcaccggat gtcacgctgg
    66181 aagatgatct taagcgtcgc gatctgacca ttaatgcgct ggcccaggac gataacggcg
    66241 agattatcga cccgtacaac ggtctgggcg atctgcaaaa tcgtctgttg cgccatgttt
    66301 cccccgcttt tggcgaagat ccgttacgcg tactgcgcgt ggcgcgtttt gctgcgcgtt
    66361 atgcccacct cggttttcgt attgccgatg aaactctgac gctgatgcgc gagatgaccc
    66421 atgcgggtga actggaacac ctgacgcctg aacgggtatg gaaagagaca gaaaacgccc
    66481 tcaccacccg caatccacag gtgttcttcc aggtactgcg cgattgcggc gcactgcgcg
    66541 ttttattccc ggaaattgac gccctgtttg gtgttccggc ccctgccagg tggcatccgg
    66601 aaatcgacac gggtattcat accttaatga cgctctcaat ggcggcgatg ctgagtccgc
    66661 aggtcgatgt ccgtttcaca actttatgcc acgatctcgg taaaggactg acgccgccag
    66721 aactctggcc gcgtcatcat ggtcatggcc cggcgggtgt taagttagtg gaacaactat
    66781 gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc cagactggtg gctgagtttc
    66841 acgatctcat ccacaccttc ccaatgctga acccaaaaac catcgtcaaa ttatttgatt
    66901 ccatcgatgc ctggcgtaaa ccgcagcgtg tcgagcaact ggcgctgacc agcgaggctg
    66961 acgtgcgcgg cagaaccggt tttgaatcag cggactaccc gcaaggccgc tggttgcgcg
    67021 aagcctggga agtggcgcag tcagtgccga caaaagccgt cgttgaagcg ggatttaaag
    67081 gtgtggagat tcgcgaggag ctgacccgac ggcggattgc ggcggtagcc agctggaagg
    67141 aacaacgttg cccaaagcct gagtgagtat tgggcatttt gccggatgcg gcgtaaacgc
    67201 cttatccggc tacatgtcaa cgacagttgt aggcctgata agacgcgcaa gcgtcgcatc
    67261 aagcgttgat tgccggacgc ggcgtgaacg ccttattcgg cctacgcaat cgtggacaac
    67321 gccaggcatc cgacactatt cctcaattaa aagaacacga catacaccgc agccgccaca
    67381 ataaagcgat aaatggcgaa cgggataaac gaaatgcgct taatcaattg caggaaggtt
    67441 ttaatcgcta tcagcgccac cacaaaagcg gtgataaacc caacggcaaa catcgggata
    67501 tcgccgcttg tcaggaagcc ccagcttttg tagagatcga gcgccgttgc gcccatcatc
    67561 atcggcaccg ccagcaggaa cgaaaactcg gaagcagcgt aacggctcac ccccatcagc
    67621 atcccacctg aaatggtcgc cccggaacgg gagaaacccg gccacagcgc cagacactgg
    67681 aaacaaccaa tcataaacgc ctgacgatag gtcatatcat caagacccgg cgcacgcggc
    67741 tctttcggct tcaggcattc ggcggcaatc agcaacaaac cgccaacgac cagcgcatac
    67801 atcacattta tcgggttaaa caatgactta atcgtgtcgt ggaacaacag ccccagtacc
    67861 accgccggaa tcatccccag caaaatgtgg atcagcgtta aacgaccttt gctttctcct
    67921 tcgtgctgca acgggcggcc aaagtggatg ccaatcaggc caaacagacg ccgccagaac
    67981 atcactacta ccgccagaat tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg
    68041 tcgccctcaa accccaacaa gtgaccgaca ataatcatat ggcccgtgct ggataccggc
    68101 agaaattctg tcaatccttc gaccacaccc aatattgccg ctatcagcag cgagtgcata
    68161 tcgctcatca ataaacccct aaattataaa aatgtaacgc ttgtccgaac tactgcagta
    68221 tgaccaggtt ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta
    68281 ttgccacgct caatgattac gccaacattc gccgcccgcg ccactgcgcc tggcttgctg
    68341 agtttgatac gcacccacgg cgagttgaag cgtgctaaca gcagctctgc cacctcttcc
    68401 gccacgcgct ccaccagcgc aaaacgtgcc ccctcgacgt ggctgaccac cgtttctgca
    68461 atgtcagcgt agctgaggca atccgccaca ttatcgcttt ttgccgcttt acggttatcc
    68521 cacgccattt cgatatcgaa cactaatttc tgttcgatgg tctgttccca gtcgtaaaca
    68581 ccaatagtgg tgattaccga aagttgctct ataaatacaa tatccatcac gacctgctcg
    68641 ctttttggct aacccggata ccacttccgg cgaaatgtgc gtattatcca cagattcatc
    68701 gtttaacacg aattttcaaa acggaacagc ttatgagtgc aatcgcgcct ggaatgatcc
    68761 tcatcgcgta cctctgcggc tccatttcca gtgccattct ggtttgccgc ctgtgtgggc
    68821 tgcccgatcc gcgaaccagc ggctccggta atcctggcgc aactaacgtg ttacgtatag
    68881 gtggcaaagg agcagccgta gcagtactga ttttcgacgt tctgaaagga atgttgcccg
    68941 tctggggcgc gtatgaatta ggtgtcagcc ccttctggct aggcttaatt gccatcgccg
    69001 cctgtcttgg acacatctgg cccgttttct tcggatttaa aggaggaaaa ggcgttgcta
    69061 ccgcttttgg tgccatcgca cccattggct gggatctcac cggagtaatg gcgggaacct
    69121 ggttactgac cgtgctattg agcggatact cgtcgctggg agcgattgtc agtgcactga
    69181 ttgctccgtt ttatgtctgg tggtttaagc cacaattcac cttcccggtt tcgatgctct
    69241 cttgcctgat cctgctgcgt catcatgaca acatccagcg tctgtggcgt cgtcaggaga
    69301 caaaaatctg gacgaaattc aaaagaaagc gcgaaaagga tcccgagtga tttctggtgg
    69361 atctacatga cctgatagcc ttcatcgggt ttgcccagcc gttgctggca ccatgccgcc
    69421 agaaattcca cgcagacacg taatttcatg ctgcgataga gcggctcccg gtaaacagcc
    69481 cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc actctccaga
    69541 aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag cgcccattgc
    69601 agcacaatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc
    69661 tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt caccagacag
    69721 tcatgacgac ttaattcctg tagggattgt ggctgcggat atttttgcag atactcaggt
    69781 gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata atcaggaatt
    69841 tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat ttgccgatcg
    69901 aacaattcaa aatacacctg taactcagga taattgcgca taagttcggt aatggctggc
    69961 gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc ctccgggcgc
    70021 gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat ttccagcgca
    70081 tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga gcggttcagc
    70141 agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtt
    70201 tgcccaagcg tcgccgctgc agcactaaaa ctcccgctgt ggacgatttc taccagtacc
    70261 tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca gttgtgataa
    70321 gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt cattcttatt
    70381 ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat ataaaaccac
    70441 atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacaaaaa ctggagttgc
    70501 catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacggaga ttgtcgctaa
    70561 ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac taaaacagct
    70621 aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt tcgacaacat
    70681 gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga ttatgttctt
    70741 cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac tcaaacaagc
    70801 cgtggaagag gcgaccatca aagcgccgct gcgtcacaat gcggtagaaa tttttgacga
    70861 agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg atatcgtccc
    70921 cgacggtgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca cgctaccagg
    70981 ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga
    71041 aaatatctcc accctcgcag taaacgcctg tccaccggta ctggtgggcg tgggcatcgc
    71101 cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attttgcgcc cgattggcag
    71161 ccgccacccc aatccaaaag cggcagagct ggagctacgc ctggaagaag gactcaaccg
    71221 tctggggatt ggtccacaag ggctaactgg caacagttca gtgatgggcg tgcatatcga
    71281 atctgccgcc cgccatccgt caaccatcgg cgttgctgtt tctaccggtt gctgggcgca
    71341 tcgtcgcggc acactgctgg ttcatgccga tctcaccttt gaaaatctgt ctcacacccg
    71401 gagcgcgtta tgaaaaagat cctgacaacc ccgatcaaag ctgaagatct gcaagatatt
    71461 cacgtcggcg atgtgatcta tctgaccggt acgctggtga cctgccgtga cgtttgtcac
    71521 cgccgattga tcgaactgaa acgtccgctc ccttacgatc tcaacggcaa agcgattttc
    71581 cacgctggtc ccatcgtgcg caaaaacggc gacaaatggg agatggtctc cgtcggtccg
    71641 acaaccagta tgcgtatgga aagttttgaa cgtgaattta tcgagcagac cggcgtgaaa
    71701 ctggtggtcg gcaaaggcgg aatggggccg ctgaccgaag aaggctgtca gaaattcaag
    71761 gcgctacatg taattttccc ggcaggctgc gcggtgctgg cggcaaccca ggtggaagag
    71821 attgaagaag tgcactggac agaactcggg atgccggagt cactgtgggt ctgccgggtc
    71881 aaagagttcg gcccgctgat tgtctctatt gatacccacg gcaacaacct gatagccgaa
    71941 aacaaaaagc tgttcgccga acgccgcgat cccatcgtgg aagagatctg cgagcacgtc
    72001 cattacatca aataaccctc ccggagaggc tcgccccctc tcctttttcg caggcataac
    72061 acgatgaaac cttccactga atggtggcga taccttgcgc cgctggcggt catcgccatt
    72121 attgctctaa ttccggttcc cgcagggctg gagagtcata cctggctcta ctttgccgtt
    72181 tttactggcg taatcgttgg actgatcctc gaacccgtgc cgggtgccgt ggtggcgatg
    72241 gtgggtatct ccatcatcgc catactctct ccctggctgc tgttcagccc ggagcagctc
    72301 gctcagccag gctttaaatt cactgcaaaa tccctctcgt gggccgtttc cggtttttct
    72361 aattcggtta tctggctgat tttcgccgcc tttatgtttg gcacaggcta tgaaaaaacc
    72421 gggctggggc gacgtatcgc gctgattctg gtgaaaaaga tggggcatcg cacgctgttt
    72481 cttggctatg cggtgatgtt ctccgagctg atcctggcac ctgtcacacc gtccaactcg
    72541 gcgcgtggtg cggggattat ctatcccatc atccgtaacc tgccgccgct ctatcaatca
    72601 caaccaaacg acagcagttc gcgcagtatt ggctcgtaca tcatgtggat ggggattgtt
    72661 gccgactgcg tgaccagcgc cattttcctg acggcgatgg ctcctaactt gctgttaatt
    72721 ggactgatga aaagcgcatc tcacgccacg ctgagttggg gcgactggtt cctcgggatg
    72781 ttgccgctca gtattttact ggtcctgctg gttccctggc tggcttacgt gctgtacccg
    72841 cctgtactga agtcaggcga tcaggtgccg cgctgggcag agacggaact gcaggcaatg
    72901 ggcccgctct gttcgcgtga aaaacggatg ctggggctga tggtaggcgc gctggtgctg
    72961 tggattttcg gcggtgatta tatcgatgcc gcgatggtcg gttacagcgt ggtggcgctg
    73021 atgctgcttc tgcgcattat cagttgggac gatattgtca gtaataaagc ggcgtggaac
    73081 gttttcttct ggctggcctc gcttatcacc ctcgcgaccg gactcaacaa caccggtttt
    73141 attagctggt ttggcaaact gttagcaggc agcttaagcg gttattcgcc aacgatggtg
    73201 atggtggcgt tgattgtggt gttttatcta ctgcgctact tttttgccag cgccacggcg
    73261 tatacctccg ctctcgcacc gatgatgatt gccgctgcgc tggcgatgcc ggaaatcccg
    73321 ctgccggtgt tctgcctgat ggttggtgcg gcaattggtc tggggagcat tcttacacca
    73381 tacgccaccg gccccagtcc gatttactac ggcagtggtt atctgccaac ggcggattac
    73441 tggcgactgg gggcgatttt tgggctgata ttcctcgtat tgctggtgat taccggctta
    73501 ctgtggatgc ccgtggtgtt gctttaagta aggacggtgc cggaagtaac tctccggcac
    73561 ccgatagttt tacgcagccg gtaactccgc cagcggccag cgcggacgca cgctaacgcc
    73621 gagatccgcc gtcgcgcctg ctttaaaccg caccattccg gcataggcga tcatcgcgcc
    73681 gttatcagta caaaattccg gacgcgcgta gaacacttcg ccgcggcgtt ttttcatcat
    73741 ttcagccagc ttcgcccgta acgtgcggtt agcactcacg ccgcccgcca tgaccagtcg
    73801 cttaaagccc gtctgatcca gcgcacgctt acacttaatc atcaacgtat cgaccaccgc
    73861 atcttcaaag gcgcgggcga tgtcagcacg cgtctggtcg tcggtgccgt tgtcacgaat
    73921 ggtatttgcc gcgaaggttt tcaggccgga gaagctgaaa tccagccccg gacggtcggt
    73981 catcggacgc gggaaaacaa agcgcccggc agtaccctgc gccgccattt tcgacagtaa
    74041 cggtccgcca ggataatcca gccccagcag cttcgcggtt ttatcaaacg cttcaccggc
    74101 ggcatcatcg atagactcgc cgagcagctc gtactgacca atgccagtca cgctgattaa
    74161 ctgcgtatga ccgccggaaa ccagcagcgc aacaaacgga aattccggcg ggttatcttc
    74221 cagcatcggc gctaacagat gcccttccat atggtgtaca gggatcgccg gaacgttcca
    74281 ggcaaacgcc agagaacgcc ccacggtcgc gccaaccagc agtgcgccga ctaatccagg
    74341 gcctgcggta taggccacag catcaatgtc ttttgccgtt aaaccagact cctttagcgc
    74401 cgcctggatc aacggtacgg ttttacgcac atgatcgcgg gaggccagtt caggcacaac
    74461 gcccccgtag tcagcgtgca atttcacctg actatacaat tggttggcta acaaaccttt
    74521 ttcatcgtcg taaatggcga tgccggtttc atcgcaggaa gtttcaatac ccagtacacg
    74581 catgacttgt tttacctcgc tttattaccg cgcagtgtag gaccaatgcg ggttgatgta
    74641 aaactttgtt cgcccctgga gaaagcctcg tgtatactcc tcacccttat aaaagtccct
    74701 ttcaaaaaag gccgcggtgc tttacaaagc agcagcaatt gcagtaaaat tccgcaccat
    74761 tttgaaataa gctggcgttg atgccagcgg caaaccgaat taatcaaagg tgagaggcac
    74821 atgccggtaa ttaaagtacg tgaaaacgag ccgttcgacg tagctctgcg tcgcttcaag
    74881 cgttcctgcg aaaaagcagg tgttctggcg gaagttcgtc gtcgtgagtt ctatgaaaaa
    74941 ccgactaccg aacgtaagcg cgctaaagct tctgcagtga aacgtcacgc gaagaaactg
    75001 gctcgcgaaa acgcacgccg cactcgtctg tactaattcc ccgagagcgt tgctctccga
    75061 tcagaccgag ttgtagttgt aaggccgtgc ttccgaaagg aatgcgcggc ttattttcgt
    75121 ttatgaattg ctaaaaatcg gggcctatgg ctggacgaat cccacgcgta ttcattaatg
    75181 atctgctggc acgcactgac atcgtcgatc tgatcgatgc ccgtgtgaag ctgaaaaagc
    75241 agggcaagaa tttccacgcg tgttgtccat tccacaacga gaaaaccccg tccttcaccg
    75301 ttaacggtga gaaacagttt taccactgct ttggatgtgg cgcgcacggc aacgcgatcg
    75361 acttcctgat gaactacgac aagcttgagt tcgtcgaaac ggtcgaagag ctggcggcaa
    75421 tgcacaatct tgaagtgcca tttgaagcag gtagcggccc cagccagatc gagcgccatc
    75481 agcggcaaac gctttatcag ttgatggacg gtctgaatac gttttaccaa caatctttac
    75541 aacaacctgt tgccacgtct gcgcgccagt atctggaaaa acgcggatta agccacgagg
    75601 ttatcgctcg ctttgcgatt ggttttgcgc ctcccggctg ggacaacgtc ctgaagcggt
    75661 ttggcggcaa tccagaaaat cgccagtcat tgattgatgc ggggatgttg gtcactaacg
    75721 atcagggacg cagttacgat cgtttccgcg agcgggtgat gttccccatt cgcgataaac
    75781 gcggtcgggt gattggtttt ggcgggcgtg tgctgggcaa cgataccccc aaatacctga
    75841 actcgccgga aacagacatt ttccataaag gccgccagct ttacggtctt tatgaagcgc
    75901 agcaggataa cgctgaaccc aatcgtctgc ttgtggtcga aggctatatg gacgtggtgg
    75961 cgctggcgca atacggcatt aattacgccg ttgcgtcgtt aggtacgtca accaccgccg
    76021 atcacataca actgttgttc cgcgcgacca acaatgtcat ttgctgttat gacggcgacc
    76081 gtgcaggccg tgatgccgca tggcgagcgc tggaaacggc gctgccttac atgacagacg
    76141 gccgtcagct acgctttatg tttttgcctg atggcgaaga ccctgacacg ctggtacgaa
    76201 aagaaggtaa agaagcgttt gaagcgcgga tggagcaggc gatgccgctt tccgcatttc
    76261 tgtttaacag cctgatgccg caagttgatc tgagtacccc tgacgggcgc gcacgtttga
    76321 gtacgctggc actgccattg atatcgcaag tgccgggcga aacgctgcga atatatcttc
    76381 gtcaggaatt aggcaacaaa ttaggcatac ttgatgacag ccagcttgaa cgattaatgc
    76441 caaaagcggc agagagcggc gtttctcgcc ctgttccgca gctaaaacgc acgaccatgc
    76501 gtatacttat agggttgctg gtgcaaaatc cagaattagc gacgttggtc ccgccgcttg
    76561 agaatctgga tgaaaataag ctccctggac ttggcttatt cagagaactg gtcaacactt
    76621 gtctctccca gccaggtctg accaccgggc aacttttaga gcactatcgt ggtacaaata
    76681 atgctgccac ccttgaaaaa ctgtcgatgt gggacgatat agcagataag aatattgctg
    76741 agcaaacctt caccgactca ctcaaccata tgtttgattc gctgcttgaa ctgcgccagg
    76801 aagagttaat cgctcgtgag cgcacgcatg gtttaagcaa cgaagaacgc ctggagctct
    76861 ggacattaaa ccaggagctg gcgaaaaagt gatttaacgg cttaagtgcc gaagagcgat
    76921 cgggaagccc ccgacagccg cactgagagg cagcggcaaa tatataagta cgccctcgta
    76981 attatcgttg gcggtaaaca accgttggat ttcagcgtta acggctgaag gacatcgggt
    77041 caatcgccca acaccaacct catgaaataa gtgtggatac cgtcttatgg agcaaaaccc
    77101 gcagtcacag ctgaaacttc ttgtcacccg tggtaaggag caaggctatc tgacctatgc
    77161 cgaggtcaat gaccatctgc cggaagatat cgtcgattca gatcagatcg aagacatcat
    77221 ccaaatgatc aacgacatgg gcattcaggt gatggaagaa gcaccggatg ccgatgatct
    77281 gatgctggct gaaaacaccg cggacgaaga tgctgccgaa gccgccgcgc aggtgctttc
    77341 cagcgtggaa tctgaaatcg ggcgcacgac tgacccggta cgcatgtaca tgcgtgaaat
    77401 gggcaccgtt gaactgttga cccgcgaagg cgaaattgac atcgctaagc gtattgaaga
    77461 cgggatcaac caggttcaat gctccgttgc tgaatatccg gaagcgatca cctatctgct
    77521 ggaacagtac gatcgtgttg aagcagaaga agcgcgtctg tccgatctga tcaccggctt
    77581 tgttgacccg aacgcagaag aagatctggc acctaccgcc actcacgtcg gttctgagct
    77641 ttcccaggaa gatctggacg atgacgaaga tgaagacgaa gaagatggcg atgacgacag
    77701 cgccgatgat gacaacagca tcgacccgga actggctcgc gaaaaatttg cggaactgcg
    77761 cgctcagtac gttgtaacgc gtgacaccat caaagcgaaa ggtcgcagtc acgctgccgc
    77821 tcaggaagag atcctgaaac tgtctgaagt attcaaacag ttccgcctgg tgccgaagca
    77881 gtttgactac ctggtcaaca gtatgcgcgt catgatggac cgcgttcgta cgcaagaacg
    77941 tctgatcatg aagctctgcg ttgagcagtg caaaatgccg aagaaaaact tcattactct
    78001 gtttaccggc aacgaaacca gcgatacctg gttcaacgcg gcaattgcga tgaacaagcc
    78061 gtggtcggaa aaactgcacg atgtctctga agaagtgcat cgcgccctgc agaaactgca
    78121 gcagattgaa gaagaaaccg gcctgaccat cgagcaggtt aaagatatca accgtcgtat
    78181 gtccatcggt gaagcgaaag cccgccgtgc gaagaaagag atggttgaag cgaacttacg
    78241 tctggttatt tctatcgcca agaaatacac caaccgtggc ttgcagttcc ttgacctgat
    78301 tcaggaaggc aacatcggtc tgatgaaagc ggttgataaa ttcgaatacc gccgtggtta
    78361 caagttctcc acctacgcaa cctggtggat ccgtcaggcg atcacccgct ctatcgcgga
    78421 tcaggcgcgc accatccgta ttccggtgca tatgattgag actatcaaca aactcaaccg
    78481 tatttctcgc cagatgctgc aagagatggg ccgtgaaccg acgccggaag aactggctga
    78541 acgtatgctg atgccggaag acaagatccg caaagtgctg aagatcgcca aagagccaat
    78601 ctccatggaa acgccgatcg gtgatgatga agattcgcat ctgggggatt tcatcgagga
    78661 taccaccctc gagctgccgc tggattctgc gaccaccgaa agcctgcgtg cggcaacaca
    78721 cgacgtgctg gctggcctga ccgcgcgtga agcgaaagtt ctgcgtatgc gtttcggtat
    78781 cgatatgaac accgatcaca cgctggaaga agtgggtaaa cagttcgacg ttacccgtga
    78841 acgtatccgt cagatcgaag cgaaggcgct gcgcaaactg cgtcacccga gccgttctga
    78901 agtgctgcgt agcttcctgg acgattaatc ggtaggccgg atcaggcgtt acgccgcacc
    78961 cggcacctgt ccctttgcac aaacgccacc ttttcggtgg cgttttttat cgcccacgca
    79021 ccaccagcgc ctggtccagc tcgcgatacg cttcaaccag tttctccagt gaaacgcgac
    79081 ttaaaccgct gggatttggc agcacccaaa tctgcgtcga accaatgctg agcgtttgtt
    79141 tcccccactg tgcaccacgc tggctgaatc cctgttcata tgcttgtttg cccagaatcg
    79201 ccaacgcttg cggctgataa tcttcaattt tttcaatcag cttacgccca cctgcgtgta
    79261 gctcttgctt tgaaatttca ttggcttgca ccgttggcct gtctaccagt ttggtgacgc
    79321 cacaacgata atccagcaga tgctgtgcct cctgcggctt caactgatgg tcggtaaacc
    79381 cggcctgata tatcaccttc cagaagcgat ttgccggatg agcaaaggga aaaccagtcc
    79441 cggcggatga aagcccaggg ttgataccgc aaaacacgac ccgtaaccct ggagccaaaa
    79501 tatcctcaac catatttact cctttctaac atcaccagga aaagtataac ggattgaaaa
    79561 tacattgttt ataaaaacag caggcgcgcg gtaatggctg gattgcgaca cggagttact
    79621 ttataatccg ctaccatggc cccttagctc agtggttaga gcaggcgact cataatcgct
    79681 tggtcgctgg ttcaagtcca gcaggggcca ccagatatag caaaggctga cgagaaatcg
    79741 tcagcctttt tctttttata tatcagttac tttgcgtgcc agtaggccgc tgcacgtacc
    79801 cgctgtgggt catactgttc cgcttcaaag cggcggctta aattcttaac gactttacct
    79861 tcgccggtta tccagatgaa gtaatcatcg gcagggattt gcatctgcgc cagacgcgca
    79921 tctaccgcct gctcatcatg tgccagccat tcgatattaa aaccatcaag gtgcgcgaga
    79981 taatcctgac aggcgttatc ccgcacgcta accagcgcac taacttgcgg tttaacggca
    80041 agtttgctca acgtttccag gcggcggcgc aatgcgggca ttccggattc atcgcagaca
    80101 tacagctgat acgcgtaatc ttccggcacc accagcgaac cgcgcggacc tgccaccgta
    80161 agtttatcgc ccggttgcgc ctgcatcgcc cagccgctgg cgaccccacc gtcgtgaata
    80221 aagaaatcga tcgccagttc atggcgtagt tcgtcataca gcggcgtata gtcacgcgac
    80281 ggtgggcgtg gtccttccgg ccagacgatg ccctcttccg ttaccgttgg cggcacaaag
    80341 tgagcgtcag gttgaggaaa gaagagtttg ctgtgatcgt caaagccgcg cgatgtaaaa
    80401 ccgtccagcg cctcgccgcc aaggacaatg cgctgaaaac cggcgctgat acgctcaacg
    80461 cgtagcacag tcaattcacg gaagcgcaga tcattgcgaa cgcgctgcgg gtagcggggg
    80521 gagttattca ttgttatcgc cttcgtgatg gtaatcagat atatctaaat aattttcgca
    80581 aatgataatg attgttaatc atgataaatg caagcgattt gtagaactga tatgtctata
    80641 gtctgacaag acgaaccgcc tcttctcagg catcattact caacgccgga tgcggcgtga
    80701 acgccttatc cggcctacgt gtgagatgag tccacattta aaaatcattt ttattcttgc
    80761 aagagtgctc acatcagata taaattagat atatctaatt aagcaaaagg aggctgatat
    80821 gagccatcat cacgaagggt gttgtaaaca tgaaggccag ccacgccatg agggctgctg
    80881 caaaggtgag aagtcagaac acgagcactg cggacacggt caccagcatg aacacggtca
    80941 atgttgcggt ggtcgccacg gtcgcggcgg cggtcgtcgg caacgtttct ttggtcacgg
    81001 tgaattgcgt ctggtgattc tggatattct ctcgcgcgat gacagccacg gttacgaatt
    81061 gattaaagcg attgagaatc taacccaagg gaattacacc ccaagcccgg gcgtcatcta
    81121 cccgacgctg gattttctgc aggagcagtc gctgattacc atccgcgaag aggaaggtgg
    81181 taagaagcag attgcgctga ccgaacaagg cgcgcagtgg ctggaagaaa accgcgaaca
    81241 ggtggagatg attgaagaac gcatcaaagc gcgttgcgtt ggcgctgcgc tgcgccagaa
    81301 cccgcaaatg aagcgggcgc tagataattt taaagcggtg ctgaatttac gcgtcaacca
    81361 gagtgatatc actgatgcac aaataaaaaa gatcattgcg gtgatcgacc gcgccgcttt
    81421 tgatattacg caactggatt aatcgccgca tccgccagtg gcgcggtgca attgccggat
    81481 gcgacgcttg acgcacctta tccggcctac acccgctaca caccccgtag gcctgataag
    81541 atgcgccagc atcgcatcag gcatcgtgta tcaaataccg gatccggcat accgattaat
    81601 gcagcaccgt caccgcgtct tccagtcggc tggcgcggtg tttcaccatc gccgacacct
    81661 gcgcactctc ttccaccagc tcggcatttt tctgggtgat gaggttaagc tcatccactg
    81721 cacgggtcag gctggaaaga ccatcggcct gttctagcgt tgaatggcta atctgggcaa
    81781 tcaactgggt gacgtttttc acctgtgcca caatatcttc catcgtacgt ccggcggcgt
    81841 gtacctgctg cgaaccggat tgcaccttat cagcactggc atcaatcagc ttgcgaatat
    81901 cgttggcagc attggcgctg cggctggcta aatgacgcac ttcccccgcc accaccgcaa
    81961 aacctttgcc ctgttcgccg gcacgcgccg cttccaccgc cgcattcagg gccagaatat
    82021 tggtctgaaa cgcaatatcg ttaatcagcg aagtaatggt gccaatgcgc tgggtactgt
    82081 cggcgatatc gtccattgtc ttgatcaccg tggtcatcgc ctccccgccc tgcactgccg
    82141 cattactggc ggtgattgac agtttatcgg cagccgacgc cgtggcagag ttctgtttca
    82201 ccgacgccgc catttggttc atggtcgcca ccgtttgctg aacgttatca actgtctgct
    82261 gggtatgttc gttcagttca tcggtgcctt tcgccagcgt ctcactgccg tttctgacac
    82321 tggacacctg gcttgagaca tcgttaatta accaacggca catcaggcca agctgcccta
    82381 ccgcgcgtaa tgtcagcccc agctcatcgc tgcgattcag atgctcaaca ctattacgct
    82441 ctccggtcgc caccttcagt gcctgacggg cgacattttc tatcgggcgg acaatctgcc
    82501 attcaaaaca ggcgcttgcc aacaatacca ctaacgcaca gaggatatac gtcaccaccg
    82561 gggcagcaac aaaccaaagc atggccgcca gcaagataaa catcagggtc atcactccac
    82621 gcgcccgcca gcgaagcggt aatgaaggca gtttacccag ccagccttta cgcaccacca
    82681 ggcctttatg aatacgctta ccggtacgtc cggcgttcag cgctttgtac agcggctcca
    82741 ccgccgcgat ctcttcatcc gtcgcccggg tacgaatcga catatagcca ctgatttttc
    82801 cctcgcgcac catcggtacc gcattggccc gcacccaata atggtcacca tttttgcggc
    82861 gatttttcac gatgccgctc cagggctccc cttttttcag ggtgaaccac atatccgcaa
    82921 acgccgcttt tggcatatcc gggtgacgca ccatgttgtg cggctgccct tgtaactctt
    82981 gcaaggtaaa gccgctcacc tgcacaaaag tgtcattagc atgagtgata tagctttgca
    83041 gatcggtagt ggacatcaga gtggtatcgt ccgccagcgg ggtattttgc tgggtgacat
    83101 acggatgaga agacatgttc gcgtcctgtg caggttatat ggttgttaac ttcttgtcag
    83161 agtttatgtc ggccccgctg cggttatctt taaccgatta atttgattta gatcgcaatt
    83221 tgcgattaaa gcacaaatct aattcgttga tttaaaatac tttcactctg ttactatacg
    83281 aaaacgttaa ttatcttgcc caaaaatcag gcagttaatg ccctgaaagc gtgcatttgc
    83341 gcagcaatta tcaaatccat acccgacaaa aaccgtgcaa aataacaaca gatgttaaca
    83401 aatagcatta aatattgcgc aaatgataac cacatttgtg tttatcccga ttttcgcgat
    83461 cgcagccgga gtggcgcaat ccctgcaata cttaaatcgg tatcatgtga tacgcgagcc
    83521 tccggagcat attttgaaca ggttaccttc gagcgcatcg gctttagcgt gcagcgccca
    83581 cgccctgaat ctcattgaaa agcgaacgct ggatcatgag gagatgaaag cacttaaccg
    83641 agaggtgatt gaatacttca aagagcatgt caatccgggg tttttagagt atcgcaaatc
    83701 tgttaccgcc ggcggggatt acggagccgt agagtggcaa gcgggaggtt taaatacgct
    83761 tgtcgacacc cagggacagg agtttatcga ctgcctggga ggttttggaa ttttcaacgt
    83821 ggggcaccgt aatccagttg tggtttccgc cgtacagaat caacttgcga aacaaccact
    83881 gcacagccag gagctactcg atccgttacg ggctatgctg gcgaaaaccc ttgctgcgct
    83941 aacgccaggt aaactgaaat acagcttctt ctgtaatagc ggcaccgagt ccgttgaagc
    84001 ggcgctgaag ctggcgaagg cttatcagtc gccgcgcggc aaatttactt ttattgccac
    84061 cagcggcgcg ttccacggta aatcacttgg cgcgctgtcg gccacggcga aatcgacctt
    84121 ccgcaaaccg tttatgccac tgctgccagg gttccgtcat gtgccgtttg gcaatatcga
    84181 agccatgcgc acggctctta atgagtgcaa aaaaaccggt gatgatgtgg ccgcggtgat
    84241 cctcgaaccg attcagggcg aaggtggcgt aattctgccg ccgccgggct atctcaccgc
    84301 cgtgcgtaag ctgtgcgatg agttcggcgc actgatgatc ctcgatgaag tacaaacggg
    84361 catggggcgc acgggcaaga tgttcgcctg cgaacatgag aacgtacagc cggacatcct
    84421 gtgcctggca aaagccctcg gcggcggtgt gatgccgatt ggcgcaacca tcgccactga
    84481 agaggtgttc tcagttctgt tcgacaaccc gttcctgcat accaccacct ttggcggcaa
    84541 cccgctggcc tgtgcggcgg cgctggcgac catcaatgtg ttgctggagc agaacttacc
    84601 ggctcaggct gagcaaaaag gcgatatgtt gctggacggt ttccgtcaac tggcgcggga
    84661 atatcccgat ctggtacagg aagcgcgtgg taaagggatg ttaatggcga ttgagtttgt
    84721 tgataacgaa atcggctata actttgccag cgagatgttc cgccagcgcg tactggtggc
    84781 cggaacgctc aataacgcca aaacgatccg cattgaaccg ccactgacac tgaccattga
    84841 acagtgtgaa ctcgtgatca aagcggcgcg taaggcgctg gcggccatgc gagtaagtgt
    84901 cgaagaagcg tagtatcaaa tcggatggca atacgacgtc gccatccgat ctttttatac
    84961 gatacgtacg cccgcaggca tcatccgctc cggcgttaac agcacgcttt cactgccgtc
    85021 atcggtttcc gcacacaaca gcatgcattc cgacgtttca ccacgcattt tcgctttttg
    85081 cagattgcat aagaccacca ccgtttttcc catcaactct tcttcgctgt agtacggcac
    85141 caggctggtc acggtttgca gtgttttttc ccccacatca acctgtacga tgtacagctt
    85201 gtcggcgttt tcatggcgtt tcacttccac aatctttccg acgcgcattt ccagacgtgc
    85261 aaaatcagcg taagccacgg tttccattgc ttcctccctt cgagtaaaat tttactaaac
    85321 tatagcaaaa gtttttctca atcctgtagg ctaaaaatgg agaatgcagg cgtgatcaca
    85381 ttcgtgagcc gctgtgttac cgttacagcg tcaaagaaac gcgctttatt tactgaaaac
    85441 aggtgacccg ataagcactt cctctacaat gggggcgcac atcagggaaa gtaaaaaagg
    85501 taaacatggc aacactaaaa gacatcgcaa tcgaagctgg cgtatccctg gcgacagtat
    85561 ccagggtctt aaatgacgat ccgacattga atgtgaaaga agagacgaaa catcgcattc
    85621 tcgagatcgc cgaaaagctg gagtacaaga ccagtagcgc ccgtaaacta cagacaggcg
    85681 cagtcaacca acaccatatt ctggctatct acagctacca gcaggagctg gagatcaacg
    85741 atccttacta tctggcgatc cgccacggca ttgaaactca gtgcgaaaag ctggcgatcg
    85801 aactgaccaa ctgttatgaa cacagcggat taccagacat taaaaacgtc accggtattt
    85861 taattgtcgg caaacccacg cccgccctgc gcgccgctgc cagcgcgttg accgacaata
    85921 tctgttttat cgactttcac gaacccggca gcggttacga tgcggtggat atcgatctgg
    85981 cacgcatcag taaagaaatc atcgacttct atatcaacca gggcgttaat cgtattggtt
    86041 ttattggcgg tgaagatgag cctggcaagg cggatattcg tgaggtcgcc tttgcggaat
    86101 atggccgact gaaacaagtg gtacgcgaag aggatatctg gcgcggcggt ttttccagtt
    86161 cgtcgggtta tgaactggca aaacaaatgc tggcgcggga agactatccg aaggcactgt
    86221 ttgttgcttc cgattccatt gctatcggcg tactgcgggc gattcatgaa cgtggtctga
    86281 acatcccaca ggatatttcg cttatcagcg ttaacgatat ccccaccgcg cgatttacct
    86341 ttccgccgct ctccaccgtg cgcatccatt ccgaaatgat gggaagtcag ggcgtaaacc
    86401 tggtgtatga aaaagcccgc gatggtcgcg cgctgccgct gttagtcttc gttcccagca
    86461 aattaaaact gcgcggcacg acccgttaaa tccccttaca cactgtccgg caatcgtttt
    86521 tgccgggcag tgctgccgtt tattttcgtg atccagttaa agtaaatgca tttacccgct
    86581 actttttagt aaaaatttta ctaaactccc cagcaattac acaaactacc atcaccatga
    86641 atggttccga tttctctcta ccgggaggcc ctatgaatcg ctgggaaaac attcagctca
    86701 cccacgaaaa ccgacttgcg ccgcgtgcgt actttttttc atatgattct gttgcgcaag
    86761 cgcgtacctt tgcccgcgaa acgagcagcc tgtttctgcc cttaagcggt cagtggaatt
    86821 tccacttttt tgaccatccg ctgcaagtgc cagaagcctt cacctctgag ttaatggctg
    86881 actgggggca tattaccgtc cccgccatgt ggcaaatgga aggtcacggc aaactgcaat
    86941 ataccgacga aggttttccg ttccccatcg atgtgccgtt tgttcccagc gataacccaa
    87001 ccggtgccta tcaacgtatt ttcaccctca gtgacggctg gcagggtaaa cagacgctga
    87061 ttaaatttga cggcgtcgaa acctattttg aagtctacgt taacggtcag tatgtgggtt
    87121 tcagcaaggg cagtcgcctg accgcagagt ttgacatcag cgcgatggtt aaaaccggcg
    87181 acaacctgtt gtgtgtgcgc gtgatgcagt gggcggactc tacctacgtg gaagaccagg
    87241 atatgtggtg gtcagcgggg atcttccgcg atgtttatct ggtcggaaaa cacctaacgc
    87301 atattaacga tttcactgtg cgtaccgact ttgacgaagc ctattgcgat gccacgcttt
    87361 cctgcgaagt ggtgctggaa aatctcgccg cctcccctgt cgtcacgacg ctggaatata
    87421 ccctgtttga tggcgaacgc gtggtgcaca gcagcgccat tgatcatttg gcaattgaaa
    87481 aactgaccag cgccagcttt gcttttactg tcgaacagcc gcagcaatgg tcagcagaat
    87541 ccccttatct ttaccatctg gtcatgacgc tgaaagacgc caacggcaac gttctggaag
    87601 tggtgccaca acgcgttggc ttccgtgata tcaaagtgcg cgacggtctg ttctggatca
    87661 ataaccgtta tgtgatgctg cacggcgtca accgtcacga caacgatcat cgcaaaggcc
    87721 gcgccgttgg aatggatcgc gtcgagaaag atctccagtt gatgaagcag cacaacatca
    87781 actccgtgcg taccgctcac tacccgaacg atccgcgttt ttacgaactg tgtgatatct
    87841 acggcctgtt tgtgatggcg gaaaccgacg tcgaatcgca cggctttgct aatgtcggcg
    87901 atatcagccg tattaccgac gatccgcagt gggaaaaagt ctacgtcgag cgcattgttc
    87961 gccatattca cgcgcagaaa aaccatccgt cgatcatcat ctggtcgctg ggcaatgaat
    88021 ccggctatgg ctgtaacatc cgcgcgatgt accacgcagc gaaggcgctg gatgacacgc
    88081 gactggtgca ttacgaagaa gatcgcgatg ctgaagtggt cgatattatt tccaccatgt
    88141 acacccgcgt gccgctgatg aatgagtttg gtgaataccc gcatccgaag ccgcgcatca
    88201 tctgtgaata tgctcatgcg atggggaacg gaccgggcgg gctgacggag taccagaacg
    88261 tcttctataa gcacgattgt attcagggac attatgtttg ggagtggtgc gaccacggga
    88321 tccaggcgca ggatgacaac ggcaatgtct ggtataaatt cggcggcgac tacggcgact
    88381 atcccaacaa ctataacttc tgtcttgatg gtttgatcta ttccgatcag acgccgggac
    88441 caggcctgaa agagtacaaa caggttatcg cgccggtaaa aatccacgcg ctggatctga
    88501 ctcgcggcga gctgaaagtc gaaaataaac tgtggtttac cacgctggat gactacaccc
    88561 tgcacgcaga ggtgcgcgcc gaaggtgaaa cgctcgcgac gcaacagatt aaactgcgcg
    88621 acgttgcgcc gaacagcgaa gcccccttgc agatcacgct gccgcagctg gacgcccgcg
    88681 aagcgttcct caacattacg gtgaccaaag attcccgcac ccgctacagc gaagccgggc
    88741 attctatcgc cacttatcag ttcccgctga aggaaaacac cgcgcagcct gtacctttcg
    88801 caccgaataa cgcccgtccg ctgacgctgg aagacgatcg tttgagctgt actgttcgcg
    88861 gctacaactt tgcgatcacc ttctcaaaaa cgagtggtaa accgacatcc tggcaggtaa
    88921 atggcgaatc gctgctgacc cgcgagccaa agatcaactt cttcaagcca atgatcgaca
    88981 accacaagca ggagtacgaa gggttgtggc agccgaatca tttgcagatc atgcaggaac
    89041 atctgcgcga ctttgccgta gaacagagcg atggtgaagt gttgatcatc agccgcacgg
    89101 ttatagcacc gccggtgttt gacttcggga tgcgctgtac ctacatctgg cgcatcgctg
    89161 cagatggcca ggttaacgtg gcgctttccg gcgagcgtta cggcgactat ccgcacatca
    89221 ttccgtgcat cggtttcacc atggggatta acggcgaata cgatcaggtg gcgtattacg
    89281 gtcgtggacc gggcgaaaac tacgccgaca gccagcaggc taacatcatc gatatctggc
    89341 gcagcaccgt cgatgccata ttcgagaact atcccttccc gcagaacaac ggcaaccgtc
    89401 agcatgtccg ctggacggca ctgactaacc gccacggcaa cggtctgctg gtggttccgc
    89461 agcgcccaat taacttcagc gcctggcact atacccagga aaacatccac gctgcccagc
    89521 actgtaacga gctgcagcgc agtgatgaca tcaccctgaa tctcgaccac cagctgcttg
    89581 gcctcggctc caattcctgg ggcagcgagg tgctggactc ctggcgcgtc tggttccgtg
    89641 acttcagcta cggctttacg ttgctgccgg tttctggcgg agaagctacc gcgcaaagcc
    89701 tggcgtcgta tgagttcggc gcagggttct tttccacaaa tttgcacagc gagaataagc
    89761 aatgaggatc atcgataact tagaacagtt ccgccagatt tacgcctctg gtaagaagtg
    89821 gcaacgctgc gttgaagcga ttgaaaatat cgacaacatt cagcctggcg tcgcccactc
    89881 catcggtgac tcattgactt accgcgtgga gacagactcc gcgaccgatg cgctatttac
    89941 cgggcatcga cgctattttg aagtgcatta ctacctgcaa gggcagcaaa aaattgaata
    90001 tgcgccgaaa gagacattac aggtcgtgga atattatcgt gatgaaactg accgtgaata
    90061 tttaaaaggc tgcggagaaa ccgttgaggt cgacgaaggg caaatcgtta tttgcgatat
    90121 ccatgaagca tatcggttta tctgcaataa cgcggtcaaa aaagtagttc tcaaagtcac
    90181 catcgaagat ggttatttcc ataacaaata acaactacgg cggcaaaagg agtttgccgc
    90241 caccgctacc ctactcattt tcggagatgt gttatgtctg ataccaaacg taatacaatc
    90301 ggcaaattcg gcttgctctc gctgactttt gccgccgttt acagctttaa caacgttatc
    90361 aataataata ttgagcttgg actggcctcg gcaccgatgt ttttcctcgc gacgattttt
    90421 tattttattc ccttctgtct gatcatcgca gaatttgttt cgttaaataa aaactcagaa
    90481 gccggtgtct acgcgtgggt aaaaagttcg ctgggcggac gttgggcatt tattactgcc
    90541 tatacctact ggttcgtaaa cctgttcttt ttcacctcgc tgttgccgcg cgttattgct
    90601 tatgcttcgt atgccttcct cggctatgaa tatattatga cgccggttgc caccaccatt
    90661 atcagtatgg tgctgttcgc cttctccacc tgggtttcca ccaacggggc gaaaatgttg
    90721 gggccaatta cctccgtcac ttcaacgctg atgctgctgt taacgctctc ctacatttta
    90781 ctggcaggta cggcgctggt tggcggcgta cagcctgctg accccatcac cgttgacgcg
    90841 atgatcccga acttcaactg ggcgttcctc ggcgttacca cctggatctt tatggccgca
    90901 ggtggcgcgg agtccgtcgc tgtgtacgtt aacgacgtca aaggcggttc gaaatcgttc
    90961 gttaaagtga tcatcctcgc cgggattttt atcggtgtac tatattccgt ctcctcggtg
    91021 ctgattaacg tcttcgtcag cagcaaagag ttgaaattta ctggcggatc ggtgcaggta
    91081 ttccacggca tggcggcgta ttttggtctg ccggaagcgt tgatgaatcg ctttgtcggt
    91141 ctggtgtcct ttaccgcaat gttcggttcc ctgctgatgt ggaccgcaac gccggtgaaa
    91201 attttcttct ccgaaatccc ggaaggcatc tttggtaaga aaaccgtcga acttaacgaa
    91261 aacggcgttc cggcgcgcgc agcgtggatc cagttcctga tcgtcatccc gctgatgatt
    91321 atcccgatgc tcggttccaa tactgtgcag gatctgatga atactattat taatatgacc
    91381 gccgcagcgt ccatgcttcc gccgttattc atcatgctgg cttacctgaa tttacgcgcc
    91441 aaattagatc acctgccacg cgatttccgt atgggctccc gccgcaccgg tattatcgtt
    91501 gtttcaatgc tgattgcgat atttgccgta gggtttgtcg cttcgacatt cccgactggc
    91561 gcgaatattc tgaccatcat tttttataac gtcggcggta ttgttatatt ccttggcttt
    91621 gcgtggtgga aatacagtaa atatataaag ggattaacgg ctgaagagcg ccatattgaa
    91681 gcgacgccag ccagcaatgt tgattaaaca gactaataaa aaatgagcag gcattttccc
    91741 tcttcctgga tgaggagagg gaaaatagtt tctgccttta tatttttata attacaacga
    91801 taaaaggctg tactttttct ttagctcatg gattaacaca atgaaattaa tcactgcacc
    91861 atgcagagca ttacttgctc tgccgttttg ctacgccttt tctgcggcag gagaagaagc
    91921 acgtccggca gaacatgacg acacaaaaac ccccgcaatt acctcgacat cttctccttc
    91981 atttcgtttt tacggcgaat taggggttgg tggatatatg gatttagagg gtgagaataa
    92041 acataaatac agcgacggca cctatattga aggtggcctg gagatgaagt acggctcctg
    92101 gttcggcctg atttacggcg aaggctggac cgtgcaggcc gaccacgacg gcaatgcctg
    92161 ggtgcctgac catagctggg gcggtttcga aggtgggatt aaccgtttct atggcggtta
    92221 tcgaaccaat gatggcaccg aaatcatgct cagtctgcgt caggattcct cgctggatga
    92281 cctgcaatgg tggggcgact tcacacccga tctgggctac gtcattccca atacccgcga
    92341 cattatgacc gcgctgaagg tgcagaactt aagcggcaac tttcgttata gcgtcaccgc
    92401 cacacctgcc ggacatcatg acgaaagcaa agcatggcta cattttggca agtacgatcg
    92461 ctacgacgac aaatacacct atccggcaat gatgaacggt tacatccagt atgaccttgc
    92521 cgaaggcatc acctggatga atggtctgga aattaccgac ggcacaggac aactctatct
    92581 cacgggactg cttaccccta actttgccgc tcgcgcctgg caccataccg gacgtgccga
    92641 cgggctggac gtaccgggaa gtgaaagcgg gatgatggtg agcgccatgt atgaagcgtt
    92701 aaagggcgtt tatctctcca ccgcttacac ctacgccaaa catcgccctg accacgctga
    92761 cgatgaaacc acctctttca tgcagtttgg tatctggtac gaatatggcg gcggacgttt
    92821 cgcaacggct tttgatagcc gcttctacat gaaaaatgcc tctaacgatc ccagcgacca
    92881 aatcttcctg atgcaatatt tctactggta ataaggactg taatttcatg aaaatcaaaa
    92941 ctattttaac gccagtaacc tgcgctctgc tgataagttt ttccgcccat gccactaacg
    93001 ccgacaatta taaaaacgtg attaaccgta ctggtgcgcc gcagtacatg aaggattacg
    93061 attacgacga tcaccagcgt tttaatccgt ttttcgatct cggagcctgg catggtcatc
    93121 tgttgccaga cggcccgaac accatgggcg gctttccggg cgttgcgctg ctgacggaag
    93181 aatacatcaa ctttatggcc agcaatttcg accgcctgac cgtctggcag gacggcaaga
    93241 aagtcaactt cacgctggag gcatacaata ttcccggcgc gctggtgcaa aaactgacag
    93301 caaaagatgt gcaggtcgaa atgactctgc gcttcgccac gccgcgcacg tcactactgg
    93361 aaactaaaat catcagcaat aaaccgctgg atctggtgtg ggatggcgaa ctgctggaaa
    93421 aactggaagc gaaagaaggg aaaccgcttt ccgataaaac cattgctggc gaataccctg
    93481 actaccagcg caaaatcagc gccacccgtg atggcctgaa agtcaccttt ggcaaagtgc
    93541 gcgccacctg ggatctgctg acctccggtg aatcagaata tcaggtgcat aaatccctgc
    93601 cggtgcagac tgaaatcaac ggcaatcgct ttaccagtaa ggcgcatatc aacggttcga
    93661 cgacgctcta taccacctat tcccatctgc tgacctctca ggaagttagc aaagagcaaa
    93721 tgcagatccg cgatattctg gcgcgtccgg cgttttatct caccgcctcg cagcaacgct
    93781 gggaagagta tctgaagaaa gggttaacca atccggatgc gacgccggaa cagacacgcg
    93841 tcgcggtgaa agcgatcgaa acgctcaacg gtaactggcg ctcgcctggg ggtgcggtga
    93901 aatataacac cgtcacgccg tcggtgaccg ggcgctggtt ctccggcaat cagacctggc
    93961 cgtgggatac ctggaagcag gcgtttgcga tggcgcattt caatccggac atcgccaaag
    94021 agaatatccg cgcggtcttc tcctggcaga tccagcctgg cgacagcgtg cgtccgcagg
    94081 atgtgggctt tgtccccgac ctgatagcgt ggaatcttag ccccgagcgt ggtggcgatg
    94141 gtggcaactg gaacgaacga aataccaaac ccagccttgc cgcctggtcg gtgatggaag
    94201 tgtacaacgt cacccaggat aaaacctggg tggcagagat gtacccgaaa ctggtggcct
    94261 atcacgactg gtggttacgt aaccgcgatc acaacggcaa cggcgtgccg gaatatggcg
    94321 cgacccgcga caaagcccac aacactgaga gcggcgagat gctgtttacg gtgaagaaag
    94381 gcaacaaaga agagacgcag tctgggctga acaactacgc ccgcgtggtg gagaaaggcc
    94441 agtacgacag tctggaaatt ccggcacagg ttgctgcatc gtgggaatcg gggcgtgatg
    94501 atgccgccgt ctttgggttt atcgacaaag aacagctgga taaatatgtc gcaagcggcg
    94561 gcaaacgtag cgactggaca gtgaaattcg ccgaaaaccg cagtcaggac ggaacgttgc
    94621 tggggtactc gctattgcag gagtcggtgg atcaggccag ctatatgtac agcgataacc
    94681 attatctggc ggagatggcg acgattctcg gtaaaccgga agaggctaag cgctatcgcc
    94741 agttggcaca gcagctcgcg gactacatca acacctgtat gttcgacccg gctacgcagt
    94801 acttctatga cgtgcgtatt gaagataaac cgctggcgaa cggctgcgcg ggcaaaccga
    94861 ttgttgagcg cggtaaaggg ccggaaggct ggtcgccgct gtttaacggt gcggcaacgc
    94921 aggccaatgc cgacgcggtg gtgaaggtga tgctcgatcc taaagagttc aatacctttg
    94981 ttccgctggg aacggcggcg ttaaccaatc cggcctttgg cgctgatatc tactggcgcg
    95041 ggcgcgtatg ggtggatcag ttctggtttg gtctgaaagg gatggagcgt tacggttatc
    95101 gcgatgatgc cctgaaactg gcggatacgt tcttccggca cgccaaaggg ttaaccgccg
    95161 atggcccaat tcaggaaaat tacaacccgc tgacaggcgc acagcaaggc gcaccaaatt
    95221 tctcctggag tgccgcacat ttttatatgt tgtataacga ttttttccgt aagcaataat
    95281 ttgcaacggc tggcggatgc ggcgtgaacg ccttatccgc cctacatatt cgttcccgta
    95341 ggctggataa gacgcgacaa gcgtcgcatc cggcatctgc acctcgccac cggcggatgc
    95401 tgcgtgaacg ccttatccgc cctacatgtt cgttcccgta ggctggataa gacgcgacaa
    95461 gcgtcgcatc cggcatctgc acctcgccac tggcggatgc ggcgtgaacg ccttatccgc
    95521 cctacatgtt cgttcccgta ggctggataa gacccgacaa gcgtcgcatc cggcgtctgc
    95581 accgcgccac tggcggatgc ggcgtgaacg ccttatccgc cctacatgtg caatccccca
    95641 ggtcggatag ggcccggcaa gcgtcgcatc cggcgttacc accgggcgta ttctttttga
    95701 atcccatcac aaaccccgca ctcccctttt cccttttctc cggcgacggc taaattagaa
    95761 ctcatccgac cacataacaa ttattttaca tactggacaa ttttatgagc tacccgtcgc
    95821 tgttcgcccc gctggattta ggttttacca cgttaaaaaa ccgcgtgttg atgggttcaa
    95881 tgcacaccgg gctggaggaa tacccggacg gtgctgaacg gctggcagcg ttttatgccg
    95941 aacgcgcccg tcacggcgtg gcgctgattg ttagcggcgg cattgcacca gatttaacag
    96001 gcgttggcat ggaaggcggc gcaatgctca atgacgccag ccagatccca caccatcgca
    96061 ccattaccga agcggtacat caggaaggcg gcaaaatagc cctgcaaatt ttgcataccg
    96121 ggcgctacag ctaccaaccg catctggtcg caccgtccgc attgcaggcc cccatcaacc
    96181 gtttcgttcc tcatgaactc acccatgaag agatcctgca actgatcgac gatttcgccc
    96241 gctgcgcgca actggcacgg gaggcaggat acgacggtgt agaggtaatg ggttccgaag
    96301 ggtatttgat caacgaattt ctgacgctgc gcaccaatca gcgcagtgac cagtggggcg
    96361 gcgattacca caaccggatg cggtttgccg ttgaagtggt gagtgcggta cgcgaacgcg
    96421 ttggcaacga cttcattatt atctaccgac tgtcgatgct cgacctggtc gaaggcggcg
    96481 ggacttttgc cgaaacggta gagctggcgc aggccattga agcggcgggc gcgaccatta
    96541 tcaacaccgg cattggctgg catgaagcgc gtattccgac catcgccacg cccgtgccac
    96601 gcggcgcatt tagctgggtc acgcgcaaac tgaaaggtca cgtctcgctg ccgctggtga
    96661 ccaccaaccg gattaacgat ccgcaggttg ccgacgatat tctctcgcgc ggcgatgccg
    96721 atatggtatc gatggcgcga ccgtttcttg cggatgcgga actgctgtca aaagcgcaat
    96781 cagggcgagc cgatgagatc aacacctgca ttggctgcaa ccaggcgtgt ctcgatcaga
    96841 tcttcgttgg caaggtcacc tcgtgtctgg tgaatcctcg cgcctgccat gaaaccaaaa
    96901 tgccgatcct tcccgccatg cagaaaaaaa atctggcggt ggtcggtgcg ggacctgctg
    96961 ggctggcgtt tgccattaac gcggcggcgc gtggtcatca ggtaacgttg tttgacgcgc
    97021 acagtgagat cggcgggcag tttaatatcg ccaaacagat ccctggcaaa gaggagtttt
    97081 acgaaacgct gcgctattac cgccggatga tcgaagtgac gggcgtgacg ctaaaactca
    97141 atcacaccgt gacggcggat cagttacagg cgttcgatga aacgatcctc gccagtggga
    97201 tcgtgccgcg cactccgccc atcgacggga tcgatcatcc gaaggtattg agttatctcg
    97261 atgtactgcg cgacaaagcg ccggtgggca acaaagttgc catcatcggt tgtggcggga
    97321 ttggttttga tacggcgatg tatttaagtc agccgggcga atccaccagc cagaatatcg
    97381 ccgggttctg taatgaatgg gggatcgaca gcagcctaca acaggctggt ggcttaagcc
    97441 cgcagggaat gcagatcccg cgtagcccac ggcagattgt gatgctccag cgcaaagcca
    97501 gcaaaccggg acaggggtta ggcaaaacca ccggctggat ccatcgcacc accctgctct
    97561 cgcggggtgt gaaaatgatc ccaggcgtaa gttatcagaa gattgacgat gacgggctgc
    97621 atgtggtgat caacggcgaa acgcaggtat tagcagtgga caatgtggtg atctgcgcag
    97681 ggcaagagcc aaaccgcgca ttggcgcagc cgctgattga tagcgggaaa acggtgcatt
    97741 taattggcgg ctgcgatgtg gctatggagc tggacgcacg acgggcaatt gcccagggaa
    97801 cacggctggc gctggagatt taaatcgtgc attgaagtgc cggatgcaac gcatccggca
    97861 cgtcgcgtta atcaataaac aaggcgggag aaatgccgaa tcgcgttgcc aattttttag
    97921 cgtgttccag cgttaatttc ctcttcccgc tcaaaacgcg tgacaccata gatttactgc
    97981 caatttccgg cagatcggaa agggttaaac catattgatc cataagggta cgaatcacgg
    98041 ctataccgcc aggcatggct tgagccatgg cattaaattc cgcaaattcg ggcgctgatt
    98101 cttcccacgc ggttattttg gcacacacca gatccagtaa ggggttttca ggatcgttga
    98161 gcagcagatg atctaccagt tccagcgcct ggatgtattg ttcctcgttc tgaatacccg
    98221 ccagaaaagg tgccacagca gttagctttt ctcctgcatg caagatgtcg gcaatcgcaa
    98281 tcattttttc cccttagtac gatgaacagc ggtaaaaaaa tcgtattctt tatgcgtcat
    98341 aacttcacgt atgtagcact tttgcgattc aaaaaagacc attgctacaa cacgtaattc
    98401 attgccccca acattgaaaa cataatgctt atccagatat ttgaagttat ccagagatgg
    98461 gaatactgct tttaatgact caggtttttt gaaatatccc ttagcaatcg tgttccctag
    98521 agccaccaac tccgttttat gttgcgggta tttttccgca gcatctttca atgctttttg
    98581 agttatcagg tgcattcttc atcacgtccg ttgacaaatt ggcaatatga taacatccgt
    98641 tgacagattg gcaacggatg aatttatctg tgtgacggaa gaataaacgt taaacaacgc
    98701 taaacaagag aatgtagagg atggtgacag ggattttttg ttttatggag cgcgattcca
    98761 gaactgggca gacattgccg gatgcgacgc ttgccgcatc ttatccgacc tgggggtaat
    98821 gtgctaaacg tatgccagat aaggcgttta cgccttatct ggcaggtagc tgtcaatctt
    98881 accgacgacg ccccagcttc actgctttca gcaccacaaa tttattattc gtggcgatgg
    98941 tggtgcagtt gccgaaaatt ttcttcagtt tatggaagta atccaggtga cggttggcaa
    99001 cgatatacag ctcgccgttg attttcaggc agcggcgggc gtggtggaac atctcccagg
    99061 cgacgttatc ggtcagcgca tgttgctgat gaaacggcgg gttgcagagc acagcattaa
    99121 agcggaaagg ctccacgccg gagagcgcgt tgttgatcat aaattcgcag cgatccaacg
    99181 cctctggcat gttggtttca acgttcaaac ggctggaagc aaccgccatc ggcgattcat
    99241 cgacaaacac cactttcgcc tgcgggtttt tatcaagcag cgtcagacca ataacgccat
    99301 taccgcaacc gagatcgaca atctcccctt cgagattctc tggcagatgt tgcataaaga
    99361 agcgcgcgcc gatatccagc ccggtgcggg agaagacatt cgcatggttg tggatagtcc
    99421 agtcagtacc ttccagcttc cagctaacgg tctgcggcgc atcggccagc ggtggttcat
    99481 tgaaagtgca gttaatcagg cgcgctttct tccatgccag cgtggtggtg gtcgggccga
    99541 gcactttttc gaatagttcc agcgtggaag tgtggatgtc acgggctttg gcaccggcaa
    99601 taatacgtgt ttgtggcgtg accactttgc gcagcgcacg cagttgctgt tccagcaatg
    99661 ccagtgtttt cggcactttg atcagcacca cacccggctg ttgcgggtag tcggcggtgc
    99721 tgtcgagaaa cttcacgctc gactcatcaa tcccgttgag gcgtaaattc tcgcgcgtcg
    99781 ccagttcact gatgtatgag tcgccaatgc tgtacggctt atgttccgcc agcgcgcagc
    99841 ttaacgcacc aaaggcatca ttcaggatca acaccgggcc gcggatttct gtgtcgtcca
    99901 actgttgcag caaatattca tccgccgctt cccacgcctg tagcgggtta acgtcatccg
    99961 tcgccggaaa acgttgtagt gtcagtgaac ggaaaccgtt gtctaagtgg ctcatcggcc
   100021 ctcctgaatg ataaaatttc gacattgccc ctgaaaaggg tgcgtgagta tacccttttt
   100081 ctctttttcg tgggcggtta tgagcaatct tacttatctt cagggttatc ccgagcagct
   100141 actttcccag gtgcgaacac tgattaacga acagcgtctg ggcgatgtgc tggcaaaacg
   100201 ctatccggga acacacgact acgccaccga taaagccctc tggcaatata ctcaggatct
   100261 gaaaaatcag tttctgcgta atgccccgcc gatcaataaa gtgatgtatg acaataagat
   100321 ccatgtgcta aaaaacgcgc tcgggttaca taccgctgtt tctcgtgtgc agggcggcaa
   100381 gctgaaagca aaagcagaga tccgcgtcgc taccgtgttt cgcaacgcgc cggaaccgtt
   100441 tttgcgcatg atcgtggtgc acgagctggc gcacctgaag gagaaagagc ataacaaagc
   100501 gttttaccag ttgtgctgtc atatggaacc ccagtaccac cagcttgagt tcgacacccg
   100561 actgtggcta acccagttgt cgcttgggca ggacaaaatc tgaaaatgat gtcaggcgtt
   100621 atcttttctt tctttagcgc ctaaaatcga cctcccccct ttcgttaagg ataacgacca
   100681 tgctgcgcgc atttgcccgc cttcttctcc gtatttgttt ttctcgccgg actctaaaga
   100741 ttgcctgctt actgttactt gtggcaggag cgaccatttt tatcgccgat cgcgtaatgg
   100801 tcaacgccag caaacagctg acctggagcg acgtcaacgt cgttccggcg cgtaacgtgg
   100861 gcttattgct gggggccagg ccgggtaatc gctactttac ccgacgtatt gataccgcag
   100921 cagagttgta ccacgccgga aaagtgaaat ggctgctggt cagtggcgat aacggacgta
   100981 aaaattacga tgaagcgtca ggcatgcagc aggctttgat cgcaaagggc gtaccggcaa
   101041 aagtgatctt ctgtgactat gccggattct caacgcttga ttcagtggtg cgtgccaata
   101101 aagtgtttgg ggagaaccat atcaccatta tctcgcagga atttcataac caacgcgcca
   101161 tctggctggc aaaacaatac ggcatcgatg ctatcgggtt taacgcacca gacctggaaa
   101221 aaggacgcgg aaagattgtc cgtctgcgag agaaactcgc cagagtcagc gcggtgattg
   101281 acgcgaaaat cttcaatcgt cagccaaaat atctcgggcc gtcggtcatt attggtccgt
   101341 ttagtgagca tggttgtccg gcgcagaaat aatgcgtatc cgcgcacgtc gaagatgaaa
   101401 aaggcgtgct acattgacga cagaatccct ttatggagta tccacgcgtt atgatacgtt
   101461 tcgccgtgat tggtacgaac tggataactc accagttcgt ggaggccgca catgaaagcg
   101521 gtaaatataa gttaaccgcc gtttattccc gcagccttga acaggcccag cacttcgcca
   101581 atgagttttc tgtcgagcat ctgtttactt cgctggaagc aatggcggaa agtgacgcta
   101641 tcgacgcggt gtatattgcc agcccgaacg ccctgcattt ttcccagacg caacttttcc
   101701 tgagccacaa aattcatgtg atttgtgaga aaccactggc gtcgaatctg gcggaagtgg
   101761 atgcagccat tgcctgcgcg cgtgaaaatc aggtggtgct gtttgaggca tttaaaacgg
   101821 ccagcctgcc taactttcat ctgttacgcc aggcgctgcc gaaagtcggc aaactgcgga
   101881 aagttttttt caactattgc cagtattcct cgcggtatca acgttacctg gacggtgaga
   101941 atcccaacac ctttaatccg gcattctcta acggttcaat tatggatatc ggcttttact
   102001 gcctggcgtc ggcggtagcg ttatttggtg agccgaaaag cgtgcaggca accgccagtt
   102061 tactggcaag tggtgtagat gcccacggcg tggtggtgat ggattacggt gatttcagcg
   102121 tcaccttgca gcactcaaag gtcagcgatt ctgtcctggc aagcgagatt cagggcgaag
   102181 cgggatcgct ggtgattgaa aaactgtctg aatgccagaa agtgtgcttc gtgccgcgtg
   102241 gcagccagat gcaggatctc acccagccgc agcatattaa taccatgctc tacgaagcag
   102301 agctgttcgc taccctggtg gatgagcatc tggtggatca tccagggctg gcggtcagtc
   102361 gcatcacagc caaactgctg accgagatcc gccgccagac tggggtgatt tttccggcag
   102421 atagcgtaaa actataattg ccaaagtaaa acagtgtaaa aggtatgtaa cagaccattg
   102481 actgactgaa tggtctgtca tactttgtta cctgcaaagg ggagtaactt cattgccggt
   102541 cgatcgtcat tacgatgtgt gaaaaaccac atccggtcac cgggcaaccc gaaaggaata
   102601 cgcagacgta ttcctttttt gttgtaagtg agaccttgcc ggaaggcgag gtctatgcat
   102661 aaaaagcagc ggctgacgtc ttccgacgtt ggccgttttt tttatgtgta aggaacttct
   102721 atgaatactg tcggcacgcc gttgctatgg ggcggattcg ctgttgttgt cgccattatg
   102781 ctggctatcg acctgttgtt gcagggacgt cgtggggcac atgccatgac catgaaacag
   102841 gctgcggcct ggtcgctggt ctgggtgacg ctgtcgttac tgtttaacgc tgctttctgg
   102901 tggtatctgg tacaaaccga aggtcgcgcc gttgccgatc cacaggcgct ggccttcctc
   102961 acgggttatc tgattgagaa gtcgctggcg gtcgataacg tctttgtctg gctgatgttg
   103021 ttcagctatt tctccgttcc ggcggcatta caacgccgcg tgctggtgta tggcgtgctc
   103081 ggggcgattg ttctgcgtac catcatgatc ttcactggca gctggctgat ttcgcagttc
   103141 gactggatcc tgtatatctt cggtgccttc ctgctgttta ccggcgtgaa gatggcgctc
   103201 gcccatgaag atgaatcagg cattggcgac aagccgctgg tgcgctggct acgcggtcat
   103261 ttgcgcatga ccgacaccat cgacaacgag catttctttg tgcgtaagaa tggattgctg
   103321 tacgccacac cgctgatgct ggtactgatt ctggtggaat tgagcgacgt gatttttgcc
   103381 gtggatagca ttccggctat cttcgccgtg accactgacc cgttcattgt gctgacctca
   103441 aacctgtttg cgatcctcgg cctgcgtgcg atgtatttcc tgctggcggg cgtagcagag
   103501 cgtttctcga tgctcaaata tggcctggcg gtgattctgg tgtttatcgg tatcaagatg
   103561 ctgattgtcg acttctacca tattccaatc gccgtctcgc tgggcgtggt gtttggcatc
   103621 ctggtgatga cgtttattat caacgcctgg gtgaattatc ggcatgataa gcagcaggtt
   103681 ggatgatttt taatctgcct aagccgtgta ccctgtcatc aacatgagca ccgttttctc
   103741 cctctccctt ccagggagag ggtcggggtg agggtaattt ttcgcaccga tgctggcctg
   103801 ttcccctcac cctaaccctc tccccaaacg gggcgagggg actgaccgag tccttttttg
   103861 acgtagtcct cagtctggaa gccgcacgtt ggctttattt ttatgtcaaa taaatgtaac
   103921 cattaagttg caaaatatga cctctcttta aaatccagca tttttcgctt cccgaagctg
   103981 taactttcct tatactcgac cttgcaaaca ctttgttaca tcctgaaaga tgcgtcgaca
   104041 gaacgcacca gggatgtgcg acaacacaat gaaaggatcg aaaaatgact acgcaacgtt
   104101 caccggggct attccggcgt ctggctcatg gcagcctggt aaaacaaatc ctggtcggcc
   104161 ttgttctggg gattcttctg gcatggatct caaaacccgc ggcggaagct gttggtctgt
   104221 taggtacttt gttcgtcggc gcactgaaag ccgttgcccc catcctggtg ttgatgctgg
   104281 tgatggcatc tattgctaac caccagcacg ggcagaaaac caatatccgc cctattttgt
   104341 tcctctatct gctgggtacc ttctctgctg ctctggccgc agtcgttttc agcttcgcct
   104401 tcccttctac cctgcactta tccagtagcg cgggtgatat ttcgccaccg tcaggcattg
   104461 tagaagtgat gcgcgggctg gtaatgagca tggtttccaa ccccatcgac gcgctgctga
   104521 aaggtaacta catcgggatc ctggtgtggg caattggcct cggtttcgca ctgcgtcacg
   104581 gtaacgagac caccaaaaac ctggtcaacg atatgtcgaa tgccgtcacc tttatggtga
   104641 aactggtcat tcgcttcgca ccgattggta tttttggtct ggtttcttct accctggcaa
   104701 ccaccggttt ctccacactg tggggctacg cgcaactgct ggtcgtgctg gttggctgta
   104761 tgttactggt ggcgctggtg gttaacccat tgctggtgtg gtggaaaatt cgtcgtaacc
   104821 cgttcccgct ggtgctgctg tgcctgcgcg aaagcggcgt gtatgccttc ttcacccgca
   104881 gctctgcggc gaacattccg gtgaatatgg cactgtgtga aaagctgaat ctggatcgcg
   104941 atacctattc cgtttctatt ccgctgggag ccaccatcaa tatggcgggc gcagcaatca
   105001 ccattaccgt gttgacgctg gctgcggtta atacgctggg tattccggtc gatctgccca
   105061 cagcgctgct gttgagcgta gtggcttctc tgtgtgcctg tggcgcatcc ggcgtggcgg
   105121 ggggttcttt gctgctgatc ccactggcct gtaatatgtt cggtatttcg aacgatatcg
   105181 ccatgcaggt ggttgccgtc ggctttatta tcggcgtatt gcaggactct tgcgaaaccg
   105241 cgctgaactc ttcaactgac gtgctgttca ctgcggcagc ttgccaggcg gaagacgatc
   105301 gtctggcaaa tagcgccctg cgtaattaat tgtttaaccc ctttcgtcta tggcgggagg
   105361 ggttttctct actttaaacg gatcaattcc ccttttctgc atccgccaga aacgaatgat
   105421 attcaggcca ttcataagca gaaaactacc ctcaatcatc gtgccgccta tcgaccccgc
   105481 ccagaagttg tgaatcaccc agcaacacgt tgaaaaccac atcacgcagc gcatggtgag
   105541 ccctttacag cggaacagcg cccaggtact gacaatcgta ccgataaccg gcaatagttc
   105601 gacaggatga tggaacttcg cgaggccaat tccgccagtc agcacaataa aaatcgccat
   105661 tacccataag ctgcgcgtgc gtaaagtaat caatgtacga atggcattaa ggatggcact
   105721 ggcaccagcg ggataggtgc ccagaagaaa aaaatgtacg ccaataacgg cgctatagac
   105781 cgaaagctgc tttttgaagc gacgttcgtc acgattgaaa aatgttgtga taccaatcag
   105841 aaaggcgatg acacccacgc cctgggccag ccaatacgcg gtcatgataa atccttatca
   105901 ggtatggaaa agcaaacggc gcttcacatt atgaaacgcc gttttttatt aacaactcat
   105961 ttcgacttta tagcgttacg ccgcttttga agatcgccag ttcgcggaag tcgttacgct
   106021 cgttgcaggt ttgtttaccg ttggcaaact caacgatggt atcgataaat tcttccagca
   106081 actgcggcat cgctttaccg tggatcagct gacccgcgtc aaagtcgatc cagtgttttt
   106141 tcttcgccgc cagttcactg ttggtagcga ttttcaccgt cggtacaaag ccgccgtatg
   106201 gcgtgccacg accagtactg aacagcacca tatggcagcc cgcacccgcc agggcgctgg
   106261 tcgctacggc atcgttaccc ggcgcactta acaagttcag ccccggcgtt ttcagacgct
   106321 cgccgtaacg cagcacgtca accacgacgc tggaacccgc tttctgggta cagccaaggg
   106381 atttatcttc cagcgtggtg ataccgcccg ctttgttccc cggcgatggg ttttcataga
   106441 tcggctggtc atgggcaata aagtactgtt tgaagtcgtt gaccatggtg accagttttt
   106501 caaacgttgc ttcgtcgcgg caatggtcca tcagcaactg ctctgcgcca aacatctccg
   106561 gcacttcggt cagtacggta gtaccgccgt tagcaatcac gtagtcagag aaacgcccca
   106621 gcatcgggtt agcagtaata ccagaaagac cgtcagaacc accgcactcc agaccaaact
   106681 tcagttcgct gagtttgcct ggctcgcgtt tatcgttgcg catcacgtta tacagctgat
   106741 gcaaatgctc gattccggct tcgatctcat catcctgctg ttggcagatc atgaaatgaa
   106801 cgcgttcagg atcgatatcg cccagcgttt cacggaatgc ggcaacctgg ttgttttcac
   106861 agcccagacc aatcaccagc actgcgcctg cgttcgggtg gcgcaccatg ttttgcagca
   106921 tggtgcgggt attaatgtga tcgtcgccca gctgtgagca gccgtaggtg tggctgaaga
   106981 ggaacacgcc gtcggtacct tcagcgttgt tggtctcttt cagaaaacgg ttctggatct
   107041 gccgcgcgat gccgttgaca cagcccacgg ttggcaggat ccacagctcg ttacgcaccc
   107101 cgacatcgcc gttagcgcga cgatagatct gcacttcacg atctgccgct tgcgcaggca
   107161 gatcctgaaa atcaggttga tagcgatact gatccagatc gctcagattc gtgcgcgtat
   107221 tgtgggcgtg aacgtgttcc cccgccgcaa tatccgccaa tgcataacca atcggcaggc
   107281 catatttgat gacgttggcc ccttttgcga tattcgttaa cgcaaattta tgtccacgag
   107341 caacatcctg gcgcagccta acagtttggt tatcgacact gacttctgtg ccttcagcca
   107401 aatccgctaa agcgaccgcg acgttatcca gcgcatggat cttgatgtat tgcatatcaa
   107461 ccccagacct tagttcagtt caatggcgaa gtagtcacgc gcattgttaa agcaaatatt
   107521 tttcaccatc tcgcccagca ggttgatgtc cgccggtgct tcgcccgctt ccacccagcg
   107581 accgatcatc tggcacagaa tgcggcggaa gtattcgtga cgggtgtatg acaggaagct
   107641 acggctgtca gtcagcatac cgacaaagcg gctcagcaaa ccgagctgcg ccagttgggt
   107701 catctgacgt tccataccgt ctttctgatc gttaaaccac cagccggaac cgaactgcat
   107761 tttgcccggc ataccttcgc cctggaagtt accgctcatg gtgcccagca cttcgttatc
   107821 gcgcgggttc aggcagtaca gaatggtttt cggcagcagg ttttcttcgt tctgcttgct
   107881 cagcagctta gacaactctt ctgccatcgg acggtcgttg atggagtcaa agcctacatc
   107941 cggccccagc agtttgaact gacgcaggtt gttattacgc agcgcgccaa tgtggtactg
   108001 ctgtacccag ccgcgacgtg catattcggc accaaggaac accagtaccg cagttttgaa
   108061 ctgtgccact tcgtgctcgc tcagggtttc gccagccaga cggcgcgcca gaatgctgtc
   108121 caattccgct tcgttcgctt cagcaaacat caccacgtcc agcgcatggt cagacacttt
   108181 acagccgtga gcggcgaagt gatccagacg tttggtcaag gcagtttgca ggtcagcaaa
   108241 gcggcgaatg tcggtatcgg aaacttcgcc cagcttcgcc atgtagtcgt taaaggtcgc
   108301 ctgttcgatg ttgaaggctt tgtccggacg ccagctcggc agcactttga tggtgaaaga
   108361 gccgtctttg gcgatctctg cgtgatgctc cagagaatcg atcgggtcat cggtggtgcc
   108421 gaccattttc acgttcatct gctgcatgat gccgcgcgcg gagaagttat cctgcgccag
   108481 cagttcgtta cattcgttcc agatttcatc ggcagttgac ggagaaagca atttgccagt
   108541 gataccaaac ggacgacgca gttcgaggtg cgtccagtgg tataacgggt tgccgatagt
   108601 gtgcggaaca gtcgccgccc aggcgtcaaa tttttcacgg tcagacgcat caccggtaca
   108661 cagacgctcg gccacaccgt tggtacgcat agcgcgccat ttgtagtgat cgcctttcag
   108721 ccagatgtca tacaggtttt taaaacgata gtcttccgca atctgctgcg gcggcaaatg
   108781 gcaatggtaa tcgaaaatcg gctggtcttt tgcgtagtcg tgatacagac ggcgggcaaa
   108841 ttcggtatct aacaggaaat cttcagtcat aaacggagtc attttcgtct tcctctcaac
   108901 gagtgagcta gcttgcttat ggtgcgatgc tgacaaagtt atcacaccaa tttccagagt
   108961 ccgaagatat tttcgtgagt tagatcaata aacgtagtta aaaaaattac tctcaaagtg
   109021 gtaaatctct ctgcaggccg cgccagtact ggcctcgctg tcgtcaggta atgtccctac
   109081 aaatattcgc acatttgtga tggctctcac cttttaaagt tgtatgacaa gttatctttc
   109141 tgccgtcgca aatcataagt cgacggaatg caaattgccg attcattcat ttgttagatg
   109201 aatcgggtta accggtacgg aagccgaatt agcacgaaac tttcatggca acgttcgggg
   109261 cgtgccggtt ttttttcggt tacccggtcg taactaacat cttcagcctc tggcgggatg
   109321 atggccgcgc ttcctgcgga tataacaaaa cgatgaggtt ttacatgcgt aaaattaaag
   109381 ggttacgttg gtatatgatc gcactggtga cgctcggcac cgtgcttggt tacctgacgc
   109441 gtaacactgt ggcggcagct gcgccaactc tgatggaaga gttaaacatc tccacccaac
   109501 agtattccta tatcatcgca gcctattctg ctgcttatac ggtcatgcaa ccggtagcag
   109561 gttatgtact ggatgtgctg ggtacgaaaa tcggttatgc aatgtttgct gtgctgtggg
   109621 ccgtgttctg tggtgcaacc gcgctggcag gtagctgggg tggcctggct gttgctcgtg
   109681 gtgcggtcgg tgccgcggaa gcagcgatga tcccggcggg tctgaaagcc agctccgaat
   109741 ggttcccggc gaaagagcgt tccatcgcag taggctattt taacgtaggt tcttcgattg
   109801 gtgcgatgat tgcgccgccg ctggtggtat gggcaatcgt gatgcacagc tggcagatgg
   109861 catttatcat ctccggtgca ttgagcttta tctgggcgat ggcatggctg attttctata
   109921 aacatccgcg cgaccagaag catctgaccg atgaagaacg cgactatatt attaatggtc
   109981 aggaagccca gcaccaggtg agtacggcga agaaaatgtc cgttggtcag atcctgcgta
   110041 accgtcagtt ctggggtatc gcgctgccgc gtttcctggc agaaccggcc tggggtactt
   110101 ttaacgcgtg gatcccgctg ttcatgttta aagtttacgg ctttaacctg aaagagatcg
   110161 cgatgttcgc ctggatgccg atgctgtttg ctgacctcgg ttgtatcctc ggtggttacc
   110221 tgccgccgct gttccagcgt tggtttggtg tgaacctgat cgtttcccgt aagatggtcg
   110281 taacgctggg tgcagtgctg atgattggcc cgggtatgat cggcctgttc accaacccgt
   110341 atgtcgcaat tatgctgctg tgtatcggtg gttttgccca ccaggcactg tctggcgcgc
   110401 tgattacgct ctcttccgac gtgtttggtc gtaacgaagt ggcaacggcg aacggtttaa
   110461 ccgggatgtc cgcatggctg gcaagtacgc tgtttgcgct ggttgtcggt gcactggctg
   110521 acaccatcgg cttcagcccg ctgttcgcag ttctggcagt atttgacctg ctgggtgcgc
   110581 tggttatctg gaccgtattg cagaacaaac cggcgattga ggtggcgcag gaaacacata
   110641 acgatcccgc accgcaacat taacgcttcg tgtaagtttt agtttcgctt caaagccgcc
   110701 ttctcaggcg gctttttcat cactgcgagt agagctaaac tcgcaaaagt ggtataacaa
   110761 atatagtctg ccgtatcatg cctggagcgc atatggaaat cactgaacca cgccgtttgt
   110821 atcaacaact tgccgctgac ctgaaagagc gcatcgaaca gggcgtctat ctggtgggtg
   110881 ataaactgcc tgctgagcgc tttattgccg atgaaaagaa cgtcagccgt acggtagttc
   110941 gtgaagccat catcatgctg gaagttgaag gctatgtcga agtgcgtaaa ggttcaggta
   111001 ttcacgtggt ttccaaccag ccgcgccatc agcaggctgc ggacaataat atggaattcg
   111061 ccaattacgg tccgtttgag ttactccagg ctcgccaact catcgaaagt aatattgccg
   111121 agttcgcagc aactcaggta acgaaacagg acatcatgaa attgatggcc atccaggagc
   111181 aggcgcgcgg cgaacaatgc ttccgtgatt ccgagtggga tttgcagttc catattcagg
   111241 tagccctggc gacgcagaac tccgccctgg cggctatcgt tgaaaaaatg tggacccagc
   111301 gtagccataa cccgtactgg aaaaaactgc acgaacacat tgattcccgt accgtcgata
   111361 actggtgtga tgaccacgat caaatcctca aggcgctgat tcgcaaagat cctcatgctg
   111421 ctaagctggc aatgtggcag cacctggaaa acaccaagat catgttattc aacgaaacca
   111481 gcgacgactt cgagttcaat gccgaccgct atctgttcgc cgaaaacccg gtagtgcatc
   111541 tcgatacggc caccagtggc agtaaatgaa atacctgtcc gacaggcgct gcgacgcgcc
   111601 tgtttgtctc gtcggtaagc gaaaagtata aagtgtcagc ctgtgtaaat cctctcgcca
   111661 ccctcccctg cattcagcaa aatcagactc cacggacatg taattttgat aacgaacaac
   111721 gttgaccttt gttacaatta gattcaattt gaatttatgt ttttgaatgc tttcttatct
   111781 cacgatttaa cagggaatag ttcaggctgt gttgatgtat caaacccgca gaacatacca
   111841 aaacagcaat aacattgcgg tagtgcatct tttaaaacca gcgtggcgtt aaccgattca
   111901 ccaggaataa tgaatggaac ttttgaccca attgctgcaa gccctgtggg cgcaggattt
   111961 tgaaaccctg gccaatccat cgatgattgg catgttgtat tttgtcttgt ttgtaatttt
   112021 gttccttgaa aacggcttgc ttccggcggc ctttttaccg ggcgacagtt tactggtatt
   112081 ggtcggcgtg ttgattgcga aaggcgcgat gggctatccg caaacgattc tgctgctgac
   112141 cgttgccgcc agcctcggct gctgggtcag ctatattcag gggcgatggc tgggcaatac
   112201 ccgcaccgta caaaactggc tatctcattt acccgcgcat tatcatcaac gcgcacacca
   112261 tctttttcat aaacacggtt tatcggcgct gttaattggt cgctttattg cgtttgtcag
   112321 aacactgctg ccgacgattg ccgggttatc agggctgaat aacgcgcgct ttcagttttt
   112381 caactggatg agcggtctgc tgtgggtatt gatcctgaca actctgggtt acatgctcgg
   112441 caaaacgccg gtatttttaa agtacgagga ccagctgatg tcatgcctga tgctgctccc
   112501 ggtggtgctg ctggtgtttg gcctggcagg ttctctggtc gtattatgga aaaagaaata
   112561 tggaaatcgg gggtaaggga tgcaaatacc tcgcatgtcg cttcgccagc tagcctggtc
   112621 cggcgctgtt ttacttctgg tcggcacgct gttgctggcc tggtccgcgg ttcgccagca
   112681 agagtctacg ctggcgattc gtgccgttca tcaaggcaca acgatgccag acggtttttc
   112741 aatctggcat caccttgacg ctcatggcat tcctttcaaa agtatcaccc ccaaaaacga
   112801 cactctgtta attacctttg actccagcga ccagagcgcc gctgcaaaag cggtcctcga
   112861 cagaacattg ccccaaggct acatcattgc gcagcaggac aataacagtc aggctatgca
   112921 gtggctgacc cggctacggg ataactctca tcgcttcgga taacttcctg ttgtccgaaa
   112981 cagatcactc actttggtga tttcaccgta actgtctatg attaatgagg cggtggttaa
   113041 cactaccgct tcgttgtctg gacaatcgtt cctttgtaat aggtccgaat cacaatggaa
   113101 ggttcaagaa tgaaataccg catcgcttta gctgtttctc tttttgctct tagtgccggt
   113161 agttatgcca ctaccctgtg tcaggaaaag gagcaaaata tccttaagga gatcagctat
   113221 gccgaaaaac accaaaacca gaatcgtatt gacggtctga ataaagccct gagtgaagtc
   113281 cgggccaact gttcagatag ccagctgcgt gccgatcatc agaagaaaat cgcaaagcag
   113341 aaagatgagg tggcggaacg ccagcaagat ttagccgagg cgaagcaaaa aggcgatgcc
   113401 gataagattg ccaaacgcga acggaaactg gcagaagcgc aggaagagct gaaaaagctg
   113461 gaagcgcgcg actactaact cacaatagtc actacttact cacctggaga aaactatgtc
   113521 gaaagaacac actacggaac atctgcgtgc tgagttgaaa tccctttccg atacgctgga
   113581 agaggtgctt agctcatctg gcgagaagtc gaaagaagag ttgagtaaga ttcgtagcaa
   113641 agcggagcag gcactgaaac agagccgtta tcgcctgggt gaaaccggtg atgccatcgc
   113701 caaacaaacc cgtgtcgcgg cggcgcgtgc cgatgagtat gtgcgtgaaa atccatggac
   113761 gggcgtgggc attggcgctg caatcggtgt agtgctcggc gttctgctgt cgcgtcgtta
   113821 attatggcgg acactcatca cgcgcaaggg cccggtaaaa gcgttctggg catcgggcag
   113881 cgaattgttt ccatcatggt tgaaatggtg gaaacacgtc tgcggctggc ggtggttgag
   113941 ctggaagagg aaaaagctaa tctctttcaa cttttactga tgctgggcct gacgatgctt
   114001 ttcgctgcat ttggtcttat gagcctgatg gtgctaatta tttgggcggt tgatccgcaa
   114061 tatcgcctga atgcgatgat tgccaccacc gtggtgttgc tgctactggc actgattggc
   114121 ggtatctgga cgttacgtaa atcgcgtaaa tccaccttgc tgcgccatac acgccatgag
   114181 ctggcaaacg atcggcagct gctcgaggag gagtcccgtg agcagtaaag tcgaacgtga
   114241 acgacgtaag gcgcaactgc ttagccagat ccagcaacaa cggctggatc tttctgccag
   114301 tcgtcgtgaa tggctggagg caacaggcgc ttacgatcgt cgctggaata tgctgctaag
   114361 tctgcgctcc tgggcgctgg ttggcagtag cgtgatggcg atctggacga ttcgccatcc
   114421 taatatgctg gtccgctggg ccagacgcgg ttttggcgta tggagcgcct ggcgtctggt
   114481 taaaacgacc ctcaagcagc aacagcttcg cggttaactt ccctctggcc ggagccattc
   114541 cggccttacc catcaaattt tttgaagatt tttgacagtt ttccttgcta acaatcatca
   114601 ttcaccacgt ttatgattct ctccatcgac agcaacgacg ctaataccgc gcccttgcac
   114661 aaaaaaacaa tcagcagcct gagtggcgca gtggagagta tgatgaaaaa attagaagat
   114721 gttggtgtac tggtagcgcg cattttaatg ccgattctgt ttattaccgc aggctgggga
   114781 aaaattactg gttacgcggg tacccaacaa tatatggaag cgatgggcgt cccgggtttt
   114841 atgctgccgc tggtgattct gcttgagttt ggtggtggtc tggcaatcct gttcggtttc
   114901 ctgactcgca ccacagccct gtttactgcg ggctttacgc tgctgacggc atttttattt
   114961 cacagcaact ttgctgaagg cgtcaactcg ctgatgttca tgaaaaacct gacaatttct
   115021 ggcggattcc tgctgctggc aattaccggt ccgggcgcgt atagcatcga ccgcctgctg
   115081 aataaaaagt ggtaagcacg cgctatactt aacgataaaa aacgaggagg aagctcctcg
   115141 tttttgctat tggaggagag aacaatgggt caactgattg acggcgtctg gcatgacacc
   115201 tggtacgata ccaaatctac cggcggtaaa tttcaacgtt cagcttccgc atttcgtaac
   115261 tggctcactg ccgatggcgc tcctggcccc actggcacag gcggttttat cgcagagaaa
   115321 gatcgttatc atctctatgt ttcactcgcc tgcccgtggg cgcaccgcac gctgatcatg
   115381 cgcaaactca aaggactgga accgtttatt tccgtttccg tagtgaaccc gctgatgctg
   115441 gaaaacggct ggacctttga tgacagtttt ccgggagcaa ccggagacac gctctatcaa
   115501 catgaatttt tgtatcagct ttatctccac gccgatccac actacagcgg acgagttact
   115561 gttcccgtgc tgtgggacaa aaagaaccac accatcgtca gcaacgaatc agcagaaatc
   115621 atccgcatgt ttaataccgc gtttgatgcg ctgggcgcga aagcgggtga ttactaccca
   115681 ccagccctgc aaacgaaaat tgacgaactt aacggctgga tttatgacac cgttaacaac
   115741 ggcgtgtata aagctggttt tgccaccagc cagcaagcct acgacgaagc ggtggagaaa
   115801 gtgtttgaat cgctggcgcg actggaacag attttaggtc agcatcgtta cctgaccggc
   115861 aaccagctaa ccgaagccga tattcgcctg tggaccacgc tggtccgttt tgatccagtg
   115921 tatgtgaccc acttcaagtg tgataagcac cgcatcagcg attaccttaa tctgtatggc
   115981 ttcctgcgtg atatctacca gatgccggga attgccgaaa cagtcaattt cgatcatatc
   116041 cgtaatcatt acttccgcag tcataagacc atcaacccta cgcggattat ttcaattggt
   116101 ccgtggcagg atctcgatga accgcatgga cgagatgttc gcttaggtta aaaaaagcac
   116161 cttacgaggt gcctctgata attagctaag tgcatgttgt tcatgtcgga tgcggcatga
   116221 acgccttatc aaacctacaa aacttttata ttcaatatgt tgcaatgacc gcgcgggcca
   116281 gataagcgta gcgcatctgg caattttgcg cttgtcatca gtcttagcgc gccttcacag
   116341 cactttatta attccttgaa cgaatattta ctgccatttc attcacgtct attcttaatt
   116401 tgctgcttta aaaacaagtg attgagcaaa attgaggcaa aaatggactg gtatctgaaa
   116461 gtactaaaaa attatgtcgg tttccggggt cgtgcgcggc gcaaagagta ctggatgttt
   116521 attctggtca acatcatctt tacgttcgtg ctggggctgc tggataaaat gttaggctgg
   116581 caacgcgctg gtggcgaagg catcctgacg acaatctacg gtattctggt gtttttaccg
   116641 tggtgggcag ttcagttccg ccgcctgcac gacaccgacc gctcggcgtg gtgggcactg
   116701 ctattcttaa tcccgtttat cggctggctt attatcatcg tctttaactg ccaggcgggt
   116761 acgccaggcg aaaaccgctt tggacctgat ccaaaactgg aaccttaaaa caaatctgct
   116821 cgccggatgt ctgactcatc cggcttttat catcatgttg atg
//
LOCUS       DABSYT020000003       112659 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.003, whole genome shotgun sequence.
ACCESSION   DABSYT020000003 DABSYT020000000
VERSION     DABSYT020000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 112659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 112659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 112659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..112659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.003"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(161..1453)
                     /gene="ybaT"
                     /locus_tag="ID725_RS01420"
                     /old_locus_tag="pgaptmp_000284"
     CDS             complement(161..1453)
                     /gene="ybaT"
                     /locus_tag="ID725_RS01420"
                     /old_locus_tag="pgaptmp_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGV7848967.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(1456..2388)
                     /gene="glsA"
                     /locus_tag="ID725_RS01425"
                     /old_locus_tag="pgaptmp_000285"
     CDS             complement(1456..2388)
                     /gene="glsA"
                     /locus_tag="ID725_RS01425"
                     /old_locus_tag="pgaptmp_000285"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGV7848968.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            2650..5154
                     /gene="copA"
                     /locus_tag="ID725_RS01430"
                     /old_locus_tag="pgaptmp_000286"
     CDS             2650..5154
                     /gene="copA"
                     /locus_tag="ID725_RS01430"
                     /old_locus_tag="pgaptmp_000286"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGV7848969.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(5369..5710)
                     /gene="ybaQ"
                     /locus_tag="ID725_RS01435"
                     /old_locus_tag="pgaptmp_000287"
     CDS             complement(5369..5710)
                     /gene="ybaQ"
                     /locus_tag="ID725_RS01435"
                     /old_locus_tag="pgaptmp_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGV7848970.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            5848..6642
                     /gene="ybaP"
                     /locus_tag="ID725_RS01440"
                     /old_locus_tag="pgaptmp_000288"
     CDS             5848..6642
                     /gene="ybaP"
                     /locus_tag="ID725_RS01440"
                     /old_locus_tag="pgaptmp_000288"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGV7848971.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            6846..7325
                     /gene="ybaK"
                     /locus_tag="ID725_RS01445"
                     /old_locus_tag="pgaptmp_000289"
     CDS             6846..7325
                     /gene="ybaK"
                     /locus_tag="ID725_RS01445"
                     /old_locus_tag="pgaptmp_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGV7848972.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(7362..9014)
                     /gene="ushA"
                     /locus_tag="ID725_RS01450"
                     /old_locus_tag="pgaptmp_000290"
     CDS             complement(7362..9014)
                     /gene="ushA"
                     /locus_tag="ID725_RS01450"
                     /old_locus_tag="pgaptmp_000290"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGV7848973.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            9232..10452
                     /gene="fsr"
                     /locus_tag="ID725_RS01455"
                     /old_locus_tag="pgaptmp_000291"
     CDS             9232..10452
                     /gene="fsr"
                     /locus_tag="ID725_RS01455"
                     /old_locus_tag="pgaptmp_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGV7848974.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            10689..12365
                     /gene="ybaL"
                     /locus_tag="ID725_RS01460"
                     /old_locus_tag="pgaptmp_000292"
     CDS             10689..12365
                     /gene="ybaL"
                     /locus_tag="ID725_RS01460"
                     /old_locus_tag="pgaptmp_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGV7848975.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(12498..13802)
                     /gene="gsk"
                     /locus_tag="ID725_RS01465"
                     /old_locus_tag="pgaptmp_000293"
     CDS             complement(12498..13802)
                     /gene="gsk"
                     /locus_tag="ID725_RS01465"
                     /old_locus_tag="pgaptmp_000293"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGV7848976.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            13954..14913
                     /gene="aes"
                     /locus_tag="ID725_RS01470"
                     /old_locus_tag="pgaptmp_000294"
     CDS             13954..14913
                     /gene="aes"
                     /locus_tag="ID725_RS01470"
                     /old_locus_tag="pgaptmp_000294"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGV7848977.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(14910..15872)
                     /gene="hemH"
                     /locus_tag="ID725_RS01475"
                     /old_locus_tag="pgaptmp_000295"
     CDS             complement(14910..15872)
                     /gene="hemH"
                     /locus_tag="ID725_RS01475"
                     /old_locus_tag="pgaptmp_000295"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGV7848978.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(16004..16648)
                     /gene="adk"
                     /locus_tag="ID725_RS01480"
                     /old_locus_tag="pgaptmp_000296"
     CDS             complement(16004..16648)
                     /gene="adk"
                     /locus_tag="ID725_RS01480"
                     /old_locus_tag="pgaptmp_000296"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGV7848979.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(16829..18703)
                     /gene="htpG"
                     /locus_tag="ID725_RS01485"
                     /old_locus_tag="pgaptmp_000297"
     CDS             complement(16829..18703)
                     /gene="htpG"
                     /locus_tag="ID725_RS01485"
                     /old_locus_tag="pgaptmp_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGV7848980.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(18813..19418)
                     /gene="recR"
                     /locus_tag="ID725_RS01490"
                     /old_locus_tag="pgaptmp_000298"
     CDS             complement(18813..19418)
                     /gene="recR"
                     /locus_tag="ID725_RS01490"
                     /old_locus_tag="pgaptmp_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGV7848981.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(19418..19747)
                     /gene="ybaB"
                     /locus_tag="ID725_RS01495"
                     /old_locus_tag="pgaptmp_000299"
     CDS             complement(19418..19747)
                     /gene="ybaB"
                     /locus_tag="ID725_RS01495"
                     /old_locus_tag="pgaptmp_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGV7848982.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(19800..21731)
                     /gene="dnaX"
                     /locus_tag="ID725_RS01500"
                     /old_locus_tag="pgaptmp_000300"
     CDS             complement(19800..21731)
                     /gene="dnaX"
                     /locus_tag="ID725_RS01500"
                     /old_locus_tag="pgaptmp_000300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGV7848983.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    complement(20403..20467)
                     /gene="dnaX"
                     /locus_tag="ID725_RS01500"
                     /old_locus_tag="pgaptmp_000300"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(21860..22411)
                     /gene="apt"
                     /locus_tag="ID725_RS01505"
                     /old_locus_tag="pgaptmp_000301"
     CDS             complement(21860..22411)
                     /gene="apt"
                     /locus_tag="ID725_RS01505"
                     /old_locus_tag="pgaptmp_000301"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGV7848984.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(22564..22941)
                     /gene="ybaN"
                     /locus_tag="ID725_RS01510"
                     /old_locus_tag="pgaptmp_000302"
     CDS             complement(22564..22941)
                     /gene="ybaN"
                     /locus_tag="ID725_RS01510"
                     /old_locus_tag="pgaptmp_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGV7848985.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     CFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            23011..23538
                     /gene="priC"
                     /locus_tag="ID725_RS01515"
                     /old_locus_tag="pgaptmp_000303"
     CDS             23011..23538
                     /gene="priC"
                     /locus_tag="ID725_RS01515"
                     /old_locus_tag="pgaptmp_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGV7848986.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            23552..23716
                     /gene="rsmS"
                     /locus_tag="ID725_RS01520"
                     /old_locus_tag="pgaptmp_000304"
     CDS             23552..23716
                     /gene="rsmS"
                     /locus_tag="ID725_RS01520"
                     /old_locus_tag="pgaptmp_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGV7848987.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(23755..27117)
                     /gene="mscK"
                     /locus_tag="ID725_RS01525"
                     /old_locus_tag="pgaptmp_000305"
     CDS             complement(23755..27117)
                     /gene="mscK"
                     /locus_tag="ID725_RS01525"
                     /old_locus_tag="pgaptmp_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGV7848988.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(27245..27892)
                     /gene="acrR"
                     /locus_tag="ID725_RS01530"
                     /old_locus_tag="pgaptmp_000306"
     CDS             complement(27245..27892)
                     /gene="acrR"
                     /locus_tag="ID725_RS01530"
                     /old_locus_tag="pgaptmp_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGV7848989.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            28034..29227
                     /gene="acrA"
                     /locus_tag="ID725_RS01535"
                     /old_locus_tag="pgaptmp_000307"
     CDS             28034..29227
                     /gene="acrA"
                     /locus_tag="ID725_RS01535"
                     /old_locus_tag="pgaptmp_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGV7848990.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            29250..32399
                     /gene="acrB"
                     /locus_tag="ID725_RS01540"
                     /old_locus_tag="pgaptmp_000308"
     CDS             29250..32399
                     /gene="acrB"
                     /locus_tag="ID725_RS01540"
                     /old_locus_tag="pgaptmp_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGV7848991.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            32945..33319
                     /gene="tomB"
                     /locus_tag="ID725_RS01545"
                     /old_locus_tag="pgaptmp_000309"
     CDS             32945..33319
                     /gene="tomB"
                     /locus_tag="ID725_RS01545"
                     /old_locus_tag="pgaptmp_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGV7848992.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            33345..33563
                     /gene="hha"
                     /locus_tag="ID725_RS01550"
                     /old_locus_tag="pgaptmp_000310"
     CDS             33345..33563
                     /gene="hha"
                     /locus_tag="ID725_RS01550"
                     /old_locus_tag="pgaptmp_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HGV7848993.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            33735..34286
                     /gene="maa"
                     /locus_tag="ID725_RS01555"
                     /old_locus_tag="pgaptmp_000311"
     CDS             33735..34286
                     /gene="maa"
                     /locus_tag="ID725_RS01555"
                     /old_locus_tag="pgaptmp_000311"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGV7848994.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            34402..34872
                     /gene="ylaC"
                     /locus_tag="ID725_RS01560"
                     /old_locus_tag="pgaptmp_000312"
     CDS             34402..34872
                     /gene="ylaC"
                     /locus_tag="ID725_RS01560"
                     /old_locus_tag="pgaptmp_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGV7848995.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            35036..36586
                     /gene="pdeB"
                     /locus_tag="ID725_RS01565"
                     /old_locus_tag="pgaptmp_000313"
     CDS             35036..36586
                     /gene="pdeB"
                     /locus_tag="ID725_RS01565"
                     /old_locus_tag="pgaptmp_000313"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGV7848996.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(36628..36981)
                     /gene="ybaA"
                     /locus_tag="ID725_RS01570"
                     /old_locus_tag="pgaptmp_000314"
     CDS             complement(36628..36981)
                     /gene="ybaA"
                     /locus_tag="ID725_RS01570"
                     /old_locus_tag="pgaptmp_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HGV7848997.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(37101..37197)
                     /gene="ffs"
                     /locus_tag="ID725_RS01575"
                     /old_locus_tag="pgaptmp_000315"
     ncRNA           complement(37101..37197)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ID725_RS01575"
                     /old_locus_tag="pgaptmp_000315"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            37360..37671
                     /locus_tag="ID725_RS01580"
                     /old_locus_tag="pgaptmp_000316"
     CDS             37360..37671
                     /locus_tag="ID725_RS01580"
                     /old_locus_tag="pgaptmp_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGV7848998.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(37704..38276)
                     /gene="ybaY"
                     /locus_tag="ID725_RS01585"
                     /old_locus_tag="pgaptmp_000317"
     CDS             complement(37704..38276)
                     /gene="ybaY"
                     /locus_tag="ID725_RS01585"
                     /old_locus_tag="pgaptmp_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGV7848999.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            38494..39354
                     /gene="tesB"
                     /locus_tag="ID725_RS01590"
                     /old_locus_tag="pgaptmp_000318"
     CDS             38494..39354
                     /gene="tesB"
                     /locus_tag="ID725_RS01590"
                     /old_locus_tag="pgaptmp_000318"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGV7849000.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(39403..40689)
                     /gene="amtB"
                     /locus_tag="ID725_RS01595"
                     /old_locus_tag="pgaptmp_000319"
     CDS             complement(39403..40689)
                     /gene="amtB"
                     /locus_tag="ID725_RS01595"
                     /old_locus_tag="pgaptmp_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGV7849001.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(40719..41057)
                     /gene="glnK"
                     /locus_tag="ID725_RS01600"
                     /old_locus_tag="pgaptmp_000320"
     CDS             complement(40719..41057)
                     /gene="glnK"
                     /locus_tag="ID725_RS01600"
                     /old_locus_tag="pgaptmp_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGV7849002.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(41238..43019)
                     /gene="mdlB"
                     /locus_tag="ID725_RS01605"
                     /old_locus_tag="pgaptmp_000321"
     CDS             complement(41238..43019)
                     /gene="mdlB"
                     /locus_tag="ID725_RS01605"
                     /old_locus_tag="pgaptmp_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGV7849003.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(43012..44784)
                     /gene="mdlA"
                     /locus_tag="ID725_RS01610"
                     /old_locus_tag="pgaptmp_000322"
     CDS             complement(43012..44784)
                     /gene="mdlA"
                     /locus_tag="ID725_RS01610"
                     /old_locus_tag="pgaptmp_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGV7849004.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            complement(44814..45272)
                     /gene="decR"
                     /locus_tag="ID725_RS01615"
                     /old_locus_tag="pgaptmp_000323"
     CDS             complement(44814..45272)
                     /gene="decR"
                     /locus_tag="ID725_RS01615"
                     /old_locus_tag="pgaptmp_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGV7849005.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(45424..46242)
                     /gene="cof"
                     /locus_tag="ID725_RS01620"
                     /old_locus_tag="pgaptmp_000324"
     CDS             complement(45424..46242)
                     /gene="cof"
                     /locus_tag="ID725_RS01620"
                     /old_locus_tag="pgaptmp_000324"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGV7849006.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            46342..48042
                     /gene="ybaE"
                     /locus_tag="ID725_RS01625"
                     /old_locus_tag="pgaptmp_000325"
     CDS             46342..48042
                     /gene="ybaE"
                     /locus_tag="ID725_RS01625"
                     /old_locus_tag="pgaptmp_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGV7849007.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            48107..48802
                     /gene="queC"
                     /locus_tag="ID725_RS01630"
                     /old_locus_tag="pgaptmp_000326"
     CDS             48107..48802
                     /gene="queC"
                     /locus_tag="ID725_RS01630"
                     /old_locus_tag="pgaptmp_000326"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGV7849008.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(48854..49252)
                     /gene="fadM"
                     /locus_tag="ID725_RS01635"
                     /old_locus_tag="pgaptmp_000327"
     CDS             complement(48854..49252)
                     /gene="fadM"
                     /locus_tag="ID725_RS01635"
                     /old_locus_tag="pgaptmp_000327"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGV7849009.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(49358..49729)
                     /gene="ybaV"
                     /locus_tag="ID725_RS01640"
                     /old_locus_tag="pgaptmp_000328"
     CDS             complement(49358..49729)
                     /gene="ybaV"
                     /locus_tag="ID725_RS01640"
                     /old_locus_tag="pgaptmp_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGV7849010.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(49880..51751)
                     /gene="ppiD"
                     /locus_tag="ID725_RS01645"
                     /old_locus_tag="pgaptmp_000329"
     CDS             complement(49880..51751)
                     /gene="ppiD"
                     /locus_tag="ID725_RS01645"
                     /old_locus_tag="pgaptmp_000329"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGV7849011.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(51943..52233)
                     /gene="hupB"
                     /locus_tag="ID725_RS01650"
                     /old_locus_tag="pgaptmp_000330"
     CDS             complement(51943..52233)
                     /gene="hupB"
                     /locus_tag="ID725_RS01650"
                     /old_locus_tag="pgaptmp_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGV7849012.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(52424..54778)
                     /gene="lon"
                     /locus_tag="ID725_RS01655"
                     /old_locus_tag="pgaptmp_000331"
     CDS             complement(52424..54778)
                     /gene="lon"
                     /locus_tag="ID725_RS01655"
                     /old_locus_tag="pgaptmp_000331"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGV7849013.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(54966..56240)
                     /gene="clpX"
                     /locus_tag="ID725_RS01660"
                     /old_locus_tag="pgaptmp_000332"
     CDS             complement(54966..56240)
                     /gene="clpX"
                     /locus_tag="ID725_RS01660"
                     /old_locus_tag="pgaptmp_000332"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGV7849014.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(56366..56989)
                     /gene="clpP"
                     /locus_tag="ID725_RS01665"
                     /old_locus_tag="pgaptmp_000333"
     CDS             complement(56366..56989)
                     /gene="clpP"
                     /locus_tag="ID725_RS01665"
                     /old_locus_tag="pgaptmp_000333"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGV7849015.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(57234..58532)
                     /gene="tig"
                     /locus_tag="ID725_RS01670"
                     /old_locus_tag="pgaptmp_000334"
     CDS             complement(57234..58532)
                     /gene="tig"
                     /locus_tag="ID725_RS01670"
                     /old_locus_tag="pgaptmp_000334"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGV7849016.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(58876..59193)
                     /gene="bolA"
                     /locus_tag="ID725_RS01675"
                     /old_locus_tag="pgaptmp_000335"
     CDS             complement(58876..59193)
                     /gene="bolA"
                     /locus_tag="ID725_RS01675"
                     /old_locus_tag="pgaptmp_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGV7849017.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            59498..60076
                     /gene="yajG"
                     /locus_tag="ID725_RS01680"
                     /old_locus_tag="pgaptmp_000336"
     CDS             59498..60076
                     /gene="yajG"
                     /locus_tag="ID725_RS01680"
                     /old_locus_tag="pgaptmp_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGV7849018.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            60120..61595
                     /gene="ampG"
                     /locus_tag="ID725_RS01685"
                     /old_locus_tag="pgaptmp_000337"
     CDS             60120..61595
                     /gene="ampG"
                     /locus_tag="ID725_RS01685"
                     /old_locus_tag="pgaptmp_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGV7849019.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            62055..63002
                     /gene="cyoA"
                     /locus_tag="ID725_RS01690"
                     /old_locus_tag="pgaptmp_000338"
     CDS             62055..63002
                     /gene="cyoA"
                     /locus_tag="ID725_RS01690"
                     /old_locus_tag="pgaptmp_000338"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGV7849020.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            63024..65015
                     /gene="cyoB"
                     /locus_tag="ID725_RS01695"
                     /old_locus_tag="pgaptmp_000339"
     CDS             63024..65015
                     /gene="cyoB"
                     /locus_tag="ID725_RS01695"
                     /old_locus_tag="pgaptmp_000339"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGV7849021.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            65005..65619
                     /gene="cyoC"
                     /locus_tag="ID725_RS01700"
                     /old_locus_tag="pgaptmp_000340"
     CDS             65005..65619
                     /gene="cyoC"
                     /locus_tag="ID725_RS01700"
                     /old_locus_tag="pgaptmp_000340"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGV7849022.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            65619..65948
                     /gene="cyoD"
                     /locus_tag="ID725_RS01705"
                     /old_locus_tag="pgaptmp_000341"
     CDS             65619..65948
                     /gene="cyoD"
                     /locus_tag="ID725_RS01705"
                     /old_locus_tag="pgaptmp_000341"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGV7849023.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            65960..66850
                     /gene="cyoE"
                     /locus_tag="ID725_RS01710"
                     /old_locus_tag="pgaptmp_000342"
     CDS             65960..66850
                     /gene="cyoE"
                     /locus_tag="ID725_RS01710"
                     /old_locus_tag="pgaptmp_000342"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGV7849024.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            66999..68363
                     /gene="yajR"
                     /locus_tag="ID725_RS01715"
                     /old_locus_tag="pgaptmp_000343"
     CDS             66999..68363
                     /gene="yajR"
                     /locus_tag="ID725_RS01715"
                     /old_locus_tag="pgaptmp_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7849025.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(68491..68982)
                     /gene="yajQ"
                     /locus_tag="ID725_RS01720"
                     /old_locus_tag="pgaptmp_000344"
     CDS             complement(68491..68982)
                     /gene="yajQ"
                     /locus_tag="ID725_RS01720"
                     /old_locus_tag="pgaptmp_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGV7849026.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            69150..70061
                     /gene="panE"
                     /locus_tag="ID725_RS01725"
                     /old_locus_tag="pgaptmp_000345"
     CDS             69150..70061
                     /gene="panE"
                     /locus_tag="ID725_RS01725"
                     /old_locus_tag="pgaptmp_000345"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGV7849027.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            70024..70614
                     /gene="yajL"
                     /locus_tag="ID725_RS01730"
                     /old_locus_tag="pgaptmp_000346"
     CDS             70024..70614
                     /gene="yajL"
                     /locus_tag="ID725_RS01730"
                     /old_locus_tag="pgaptmp_000346"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGV7849028.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(70668..72116)
                     /gene="thiI"
                     /locus_tag="ID725_RS01735"
                     /old_locus_tag="pgaptmp_000347"
     CDS             complement(70668..72116)
                     /gene="thiI"
                     /locus_tag="ID725_RS01735"
                     /old_locus_tag="pgaptmp_000347"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGV7849029.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            72322..72564
                     /gene="xseB"
                     /locus_tag="ID725_RS01740"
                     /old_locus_tag="pgaptmp_000348"
     CDS             72322..72564
                     /gene="xseB"
                     /locus_tag="ID725_RS01740"
                     /old_locus_tag="pgaptmp_000348"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGV7849030.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            72564..73463
                     /gene="ispA"
                     /locus_tag="ID725_RS01745"
                     /old_locus_tag="pgaptmp_000349"
     CDS             72564..73463
                     /gene="ispA"
                     /locus_tag="ID725_RS01745"
                     /old_locus_tag="pgaptmp_000349"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGV7849031.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            73488..75350
                     /gene="dxs"
                     /locus_tag="ID725_RS01750"
                     /old_locus_tag="pgaptmp_000350"
     CDS             73488..75350
                     /gene="dxs"
                     /locus_tag="ID725_RS01750"
                     /old_locus_tag="pgaptmp_000350"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGV7849032.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            75405..76379
                     /gene="yajO"
                     /locus_tag="ID725_RS01755"
                     /old_locus_tag="pgaptmp_000351"
     CDS             75405..76379
                     /gene="yajO"
                     /locus_tag="ID725_RS01755"
                     /old_locus_tag="pgaptmp_000351"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGV7849033.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            76610..78181
                     /gene="espY3"
                     /locus_tag="ID725_RS01760"
                     /old_locus_tag="pgaptmp_000352"
     CDS             76610..78181
                     /gene="espY3"
                     /locus_tag="ID725_RS01760"
                     /old_locus_tag="pgaptmp_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HGV7849034.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKS
                     DGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKTEAESTEEKDISPLAKIILDYIESDKNPESLNFEEKSTVIKIKQDIDNF
                     IFYNQHLKKIFNRAMNLQEKISRKKYNEFFKYIQAEAKQYFKDQYKLTKNDYLKKVPL
                     TAQLIAKYKMDDQLDQLLVTREIQDEIKSKIQDKIDELSKNLFNTMTETIENNFDDIF
                     RQQSENMSNYYEFVD"
     gene            complement(78359..78874)
                     /gene="pgpA"
                     /locus_tag="ID725_RS01765"
                     /old_locus_tag="pgaptmp_000353"
     CDS             complement(78359..78874)
                     /gene="pgpA"
                     /locus_tag="ID725_RS01765"
                     /old_locus_tag="pgaptmp_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGV7849035.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(78852..79829)
                     /gene="thiL"
                     /locus_tag="ID725_RS01770"
                     /old_locus_tag="pgaptmp_000354"
     CDS             complement(78852..79829)
                     /gene="thiL"
                     /locus_tag="ID725_RS01770"
                     /old_locus_tag="pgaptmp_000354"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGV7849036.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(79907..80326)
                     /gene="nusB"
                     /locus_tag="ID725_RS01775"
                     /old_locus_tag="pgaptmp_000355"
     CDS             complement(79907..80326)
                     /gene="nusB"
                     /locus_tag="ID725_RS01775"
                     /old_locus_tag="pgaptmp_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGV7849037.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(80346..80816)
                     /gene="ribE"
                     /locus_tag="ID725_RS01780"
                     /old_locus_tag="pgaptmp_000356"
     CDS             complement(80346..80816)
                     /gene="ribE"
                     /locus_tag="ID725_RS01780"
                     /old_locus_tag="pgaptmp_000356"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGV7849038.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(80905..82008)
                     /gene="ribD"
                     /locus_tag="ID725_RS01785"
                     /old_locus_tag="pgaptmp_000357"
     CDS             complement(80905..82008)
                     /gene="ribD"
                     /locus_tag="ID725_RS01785"
                     /old_locus_tag="pgaptmp_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGV7849039.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(82012..82461)
                     /gene="nrdR"
                     /locus_tag="ID725_RS01790"
                     /old_locus_tag="pgaptmp_000358"
     CDS             complement(82012..82461)
                     /gene="nrdR"
                     /locus_tag="ID725_RS01790"
                     /old_locus_tag="pgaptmp_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGV7849040.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            82612..83151
                     /gene="yajI"
                     /locus_tag="ID725_RS01795"
                     /old_locus_tag="pgaptmp_000359"
     CDS             82612..83151
                     /gene="yajI"
                     /locus_tag="ID725_RS01795"
                     /old_locus_tag="pgaptmp_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGV7849041.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            83450..84334
                     /gene="tsx"
                     /locus_tag="ID725_RS01800"
                     /old_locus_tag="pgaptmp_000360"
     CDS             83450..84334
                     /gene="tsx"
                     /locus_tag="ID725_RS01800"
                     /old_locus_tag="pgaptmp_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGV7849042.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(84511..84858)
                     /gene="yajD"
                     /locus_tag="ID725_RS01805"
                     /old_locus_tag="pgaptmp_000361"
     CDS             complement(84511..84858)
                     /gene="yajD"
                     /locus_tag="ID725_RS01805"
                     /old_locus_tag="pgaptmp_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGV7849043.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(84946..85227)
                     /locus_tag="ID725_RS01810"
                     /old_locus_tag="pgaptmp_000362"
     CDS             complement(84946..85227)
                     /locus_tag="ID725_RS01810"
                     /old_locus_tag="pgaptmp_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGV7849044.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(85220..85462)
                     /locus_tag="ID725_RS01815"
                     /old_locus_tag="pgaptmp_000363"
     CDS             complement(85220..85462)
                     /locus_tag="ID725_RS01815"
                     /old_locus_tag="pgaptmp_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGV7849045.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(85614..86585)
                     /gene="secF"
                     /locus_tag="ID725_RS01820"
                     /old_locus_tag="pgaptmp_000364"
     CDS             complement(85614..86585)
                     /gene="secF"
                     /locus_tag="ID725_RS01820"
                     /old_locus_tag="pgaptmp_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGV7849046.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(86596..88443)
                     /gene="secD"
                     /locus_tag="ID725_RS01825"
                     /old_locus_tag="pgaptmp_000365"
     CDS             complement(86596..88443)
                     /gene="secD"
                     /locus_tag="ID725_RS01825"
                     /old_locus_tag="pgaptmp_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGV7849047.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(88471..88803)
                     /gene="yajC"
                     /locus_tag="ID725_RS01830"
                     /old_locus_tag="pgaptmp_000366"
     CDS             complement(88471..88803)
                     /gene="yajC"
                     /locus_tag="ID725_RS01830"
                     /old_locus_tag="pgaptmp_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGV7849048.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(88826..89953)
                     /gene="tgt"
                     /locus_tag="ID725_RS01835"
                     /old_locus_tag="pgaptmp_000367"
     CDS             complement(88826..89953)
                     /gene="tgt"
                     /locus_tag="ID725_RS01835"
                     /old_locus_tag="pgaptmp_000367"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGV7849049.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(90008..91078)
                     /gene="queA"
                     /locus_tag="ID725_RS01840"
                     /old_locus_tag="pgaptmp_000368"
     CDS             complement(90008..91078)
                     /gene="queA"
                     /locus_tag="ID725_RS01840"
                     /old_locus_tag="pgaptmp_000368"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGV7849050.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            91171..91752
                     /gene="acpH"
                     /locus_tag="ID725_RS01845"
                     /old_locus_tag="pgaptmp_000369"
     CDS             91171..91752
                     /gene="acpH"
                     /locus_tag="ID725_RS01845"
                     /old_locus_tag="pgaptmp_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGV7849051.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(91773..93218)
                     /locus_tag="ID725_RS01850"
                     /old_locus_tag="pgaptmp_000370"
     CDS             complement(91773..93218)
                     /locus_tag="ID725_RS01850"
                     /old_locus_tag="pgaptmp_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849052.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFKKSTYGKSLFI
                     SGNENLVNISAEKIDEIKNSKNFFSNIVNIIILLIKHPDKILPILHGCPVESIEVIKN
                     AKRTGQWSYEMTDKNGQSIEVSYIFTDTIDDKSDGKLCLKICNSCNQNDSSTHEYKID
                     HQQLKNHIRQLEEWALLEKFYDNNAYYSKRTKQLEKKIADAGFITRKVKAENFNVKEF
                     KDDIASMLCEQPPMRTHQISSSGYVSADKLHKLYKTNLEDAQRDLKTTPVDIDGVSLK
                     SITREKIAEEIAKKTAEIIAKQNAKSIEEKNIEKNKKQTNSEDNFVLKFSPEEINEML
                     LDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLHNVARNGSPRYAGLLRQIASKSAILQD
                     FDQQLFWRTFTGGGLHRHISVKSEPEKTDVWRFSSQFDFPHINENGELSRTVLMFEGV
                     AESNFKDIANHSSECFSYRCYAAPLDANAKK"
     gene            complement(93406..95223)
                     /gene="malZ"
                     /locus_tag="ID725_RS01855"
                     /old_locus_tag="pgaptmp_000371"
     CDS             complement(93406..95223)
                     /gene="malZ"
                     /locus_tag="ID725_RS01855"
                     /old_locus_tag="pgaptmp_000371"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGV7849053.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(95379..96752)
                     /gene="proY"
                     /locus_tag="ID725_RS01860"
                     /old_locus_tag="pgaptmp_000372"
     CDS             complement(95379..96752)
                     /gene="proY"
                     /locus_tag="ID725_RS01860"
                     /old_locus_tag="pgaptmp_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGV7849054.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(96828..98147)
                     /gene="brnQ"
                     /locus_tag="ID725_RS01865"
                     /old_locus_tag="pgaptmp_000373"
     CDS             complement(96828..98147)
                     /gene="brnQ"
                     /locus_tag="ID725_RS01865"
                     /old_locus_tag="pgaptmp_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGV7849055.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(98554..99849)
                     /gene="phoR"
                     /locus_tag="ID725_RS01870"
                     /old_locus_tag="pgaptmp_000374"
     CDS             complement(98554..99849)
                     /gene="phoR"
                     /locus_tag="ID725_RS01870"
                     /old_locus_tag="pgaptmp_000374"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGV7849056.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            complement(99907..100596)
                     /gene="phoB"
                     /locus_tag="ID725_RS01875"
                     /old_locus_tag="pgaptmp_000375"
     CDS             complement(99907..100596)
                     /gene="phoB"
                     /locus_tag="ID725_RS01875"
                     /old_locus_tag="pgaptmp_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGV7849057.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            100786..101988
                     /gene="sbcD"
                     /locus_tag="ID725_RS01880"
                     /old_locus_tag="pgaptmp_000376"
     CDS             100786..101988
                     /gene="sbcD"
                     /locus_tag="ID725_RS01880"
                     /old_locus_tag="pgaptmp_000376"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGV7849058.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            101985..105128
                     /gene="sbcC"
                     /locus_tag="ID725_RS01885"
                     /old_locus_tag="pgaptmp_000377"
     CDS             101985..105128
                     /gene="sbcC"
                     /locus_tag="ID725_RS01885"
                     /old_locus_tag="pgaptmp_000377"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGV7849059.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            105254..106438
                     /gene="araJ"
                     /locus_tag="ID725_RS01890"
                     /old_locus_tag="pgaptmp_000378"
     CDS             105254..106438
                     /gene="araJ"
                     /locus_tag="ID725_RS01890"
                     /old_locus_tag="pgaptmp_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGV7849060.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(106581..107489)
                     /gene="mak"
                     /locus_tag="ID725_RS01895"
                     /old_locus_tag="pgaptmp_000379"
     CDS             complement(106581..107489)
                     /gene="mak"
                     /locus_tag="ID725_RS01895"
                     /old_locus_tag="pgaptmp_000379"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGV7849061.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            107614..108525
                     /gene="rdgC"
                     /locus_tag="ID725_RS01900"
                     /old_locus_tag="pgaptmp_000380"
     CDS             107614..108525
                     /gene="rdgC"
                     /locus_tag="ID725_RS01900"
                     /old_locus_tag="pgaptmp_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGV7849062.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(108610..109059)
                     /locus_tag="ID725_RS01905"
                     /old_locus_tag="pgaptmp_000381"
     CDS             complement(108610..109059)
                     /locus_tag="ID725_RS01905"
                     /old_locus_tag="pgaptmp_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849063.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(109056..111335)
                     /locus_tag="ID725_RS01910"
                     /old_locus_tag="pgaptmp_000382"
     CDS             complement(109056..111335)
                     /locus_tag="ID725_RS01910"
                     /old_locus_tag="pgaptmp_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HGV7849064.1"
                     /translation="MNRINILVICMVLFFMTGNACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVAD
                     ELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVK
                     TGATRKIRNEARHRLEEKQ"
     gene            complement(111479..111763)
                     /gene="ppnP"
                     /locus_tag="ID725_RS01915"
                     /old_locus_tag="pgaptmp_000383"
     CDS             complement(111479..111763)
                     /gene="ppnP"
                     /locus_tag="ID725_RS01915"
                     /old_locus_tag="pgaptmp_000383"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGV7849065.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(111835..112512)
                     /gene="aroM"
                     /locus_tag="ID725_RS01920"
                     /old_locus_tag="pgaptmp_000384"
     CDS             complement(111835..112512)
                     /gene="aroM"
                     /locus_tag="ID725_RS01920"
                     /old_locus_tag="pgaptmp_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGV7849066.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
BASE COUNT        27305 a        29600 c        28035 g        27719 t
ORIGIN      
        1 atcatacaca ttaaaaataa tccacggtaa taagtgatta gcatcacaga tttatcgcgt
       61 atatatcatt tttttgtgac ttatttatta aaggaataag aaaggtaatt tgaattaaag
      121 acaaaggcgc agtacaggca gtactgcgcg cagagaggtg ttatacggtt ttattgcgct
      181 tcatgaccat tgccacaata aggctgagta agattgcacc aataatccat atcaacgcac
      241 ggctgccctg ggaccacagg ctgtagataa agccaacgat caccaacaac atcaccaacg
      301 tacctacgat aagaatcggc aacgaggcgt gaatatcatg acgcaggcgg atcgccacca
      361 caaacaccgc cagatagcaa atcaaaaagg tggcgctggc aacgctggcg agtgagccta
      421 aatttagtgc cgccgtcatc agcataatca acaccacgac aatgatgttg ccccaggtac
      481 tctgccgcca cagggattta ttcattagct tcggcagttc gcgttcgctg cccatgttgt
      541 ccatgatgtt aaacacggcg aacaggttcg cgttgatggc tgaagccgtc gccagtaaag
      601 cgccgatgac gacgatcaca taacccacat gcccgagcag cggagaagca gcctgcgcta
      661 cggcggtatc ggcatatttt tctaactcta atgccgatac atcgctaagc aaaaccagcg
      721 ccagcgagat ataaagcaac gtggtaacgc caattgccac cagaaacgcc cgtggcataa
      781 tgacctgcgg atctttcact ttatccgccg cgttcgccat catgccaaag cccgcatagg
      841 caaggaaggt tatcccgata caggagaaga acgcaccgga gctggggggc gcagagacgg
      901 aaatatgcgc cggttgcagc gaccagacac cggcaataat cagcaataac aggatcatca
      961 ttttaatgcc gacgaggatc acctccagcc gccctaccgc atggttgctt aaggagttga
     1021 aaagcgtcat caccgcaatg atccccaacg cgtagagcaa aataaggtgc tcctcctggc
     1081 tgccttcatg caaaaactgc acggcataag cgccaaaagc acgggcgacc atggcgatgc
     1141 tcaccgccag cgtcaacagg tacagtaacg agagcgccag cgaaaagacg ccgttgccta
     1201 atccgcgacg aaagaagtcg ataatgccgc cattgctggg atagctcgcc cccagacgcg
     1261 cataggcata accggaaaac atcgccacaa taccgccaaa agcaaaggcg acccaggtcg
     1321 aggcttccat tagcaacgca gcctgcccca gcagcgcgaa gatccccgcc cccaccattg
     1381 ccccaatgcc gatggaaacg acgttccata gaccgagggg tttgttaccg ttattacctt
     1441 ccgtgttcat catgatcagc ccttaaacac gttatagccg agttgcttag cgaccgatgc
     1501 caccattttt tgaccgcgaa cactgttgcc ttcttcgtcc agcggtggtg agaacgcggc
     1561 aattcccatc actccaggga cgaccgccag aataccgcca cctacaccgc ttttgcccgg
     1621 taaaccaaca cgatacgccc agtcaccgga gcgaccatac agcccttcca tcatcatttc
     1681 ggccagaatg tacggcacgt tgtcggcctg aagaacgcgt ttatgcgtca acggattcac
     1741 accacccgcc gccagcgtcg cgccaagcgt tgccagttca acagtattga tgagcgtgga
     1801 gcactgacgg gtgtacacgt cacaggcttc cattgcatca caatagagat atccggcgga
     1861 gtacagcagc caggcaatgg cccggttatg gaagttggtt gtttgttccg actggttgac
     1921 ttcgtcagag agcgctacca gctcaccagc cagttgctgt tggatatgta aaattcgctg
     1981 ccagcgttgt tcggcatttt cagcgttaat caggctggtg gtggcaatag cgccagcatt
     2041 taccagtggc gacagcggtt tgccgccatg caactctaag gcgataactg agttaaaggg
     2101 caatccggtc gggtcagcgc caattttgtc ctgtaccgcc tgcgggccga catcttctaa
     2161 cgcaagggct aacgtacaga ctttcgagat ggattccagc gcaaagcggt aatcactgtc
     2221 acccgcacta tagacgttgc catcgctggt cacgatagcc actgctgcca gttgaccagg
     2281 cacattcgcc agaaagggaa tgtaatcggc attttgtccg ccgttaagtg agtgaaattg
     2341 ggtgtaagcc tgatccactg cctgctgtaa tttgtttgca tctaacatct tttgttaact
     2401 cctttttata gatgcgggag gtaattcctc accccggtgc cgattttcag gcatcctgat
     2461 ttaacttagc acccgcaact taactacagg aaaacaaaga gataaatgtc taatcctgat
     2521 gcaaatcgag ccgatttttt aatctttacg gacttttact cgcctgggtt attaatttct
     2581 tgaccttccc cttgctggaa ggtttaacct ttatcacagc cagtcaaaac tgtcttaaag
     2641 gagtgtttta tgtcacaaac tatcgacctg accctggacg gcctgtcctg cggtcactgc
     2701 gttaaacgcg tgaaagaaag tctcgaacag cgcccggatg tggaacaagc ggatgtgtct
     2761 atcactgaag cgcacgttac cgggactgcc agtgcagaac agctgatcga aaccatcaaa
     2821 caagcgggtt atgacgcatc tgtaagccac ccaaaggcta aaccgctggc ggagtcatca
     2881 atcccgtcgg aagcactgac agcggtttct gaggcgcttc cggcagcgac cgccgatgac
     2941 gatgacagcc agcagttgct gctgagcggc atgagctgcg ccagctgtgt cacccgcgta
     3001 caaaatgcgc tgcaaagcgt accgggcgtc actcaggcac gggtaaacct ggcggagcgt
     3061 actgcgctgg tgatgggcag tgcctcccca caagatttag tgcaggcggt ggaaaaagcg
     3121 ggctacggcg cggaagcgat tgaagatgac gcgaaacgcc gcgagcgcca gcaagaaacc
     3181 gccgtcgcta cgatgaagcg cttccgctgg caggcaattg ttgcactggc tgtgggtatc
     3241 ccggtaatgg tctgggggat gatcggcgat aacatgatgg tcaccgctga caaccgcagc
     3301 ctgtggttgg ttatcggcct gataaccctg gcagtgatgg ttttcgccgg cggccatttt
     3361 taccgcagtg catggaaaag cctgctgaac ggtgcggcga cgatggatac gctggtggcg
     3421 ctgggtacgg gtgtggcgtg gctctattcg atgagcgtca acctgtggcc gcagtggttc
     3481 ccgatggaag cgcggcatct ttactatgaa gccagtgcga tgattatcgg tctgattaat
     3541 ctcggtcata tgctggaagc gcgcgcacgt cagcgttctt caaaggcgct ggagaaatta
     3601 ctcgatttaa ctccgccgac ggcgcgcctg gttactgacg aaggtgaaaa aagcgtgccg
     3661 ctggcggaag ttcagccagg catgctactg cgcctgacga cgggcgaccg cgtgccagta
     3721 gatggcgaga ttacccaggg cgaagcatgg ctggatgaag cgatgctgac gggcgaacct
     3781 atcccgcagc aaaaaggcga aggcgatagc gtccatgccg ggacagtggt acaggacggt
     3841 agcgttctgt ttcgcgccag tgcggttggc agccatacca cgctatcaag gattattcgt
     3901 atggtgcgcc aggcccagag cagcaagcca gaaatcggtc agctggcgga taaaatctca
     3961 gccgtatttg tgccggtagt agtggttatt gcgcttgtca gtgcggcaat ctggtatttc
     4021 tttggtccgg caccgcagat tgtctatacc ctggtgattg ccaccacggt gctgattatt
     4081 gcctgtccgt gtgcgctggg gctggcgacg ccgatgtcga ttatttccgg cgtcgggcgg
     4141 gcggctgagt ttggcgtgct ggtgcgggac gccgacgcgc tgcaacgcgc tagtacactc
     4201 gataccgtag tgttcgataa aaccgggacg ctgaccgaag ggaagccgca ggttgtcgca
     4261 gtgaaaacat ttgctgattt tgacgaagcg caggcgctgc gtctggcggc ggcactggaa
     4321 caaggttcca gccatccgct ggcgcgggcg atcctcgata aagcaagcga tatgcagctg
     4381 ccgcaggtca acggtttccg cacattgcgc gggctgggcg tgagcggtga agctgaaggt
     4441 catgcgttat tgctgggcaa tcaggcgctg ttaaatgacc agcaagttga tactaaagcg
     4501 attgaagcag atatttcagc tcaggcatcg caaggggcaa cgcctgtgct gctggcggtt
     4561 gacgggaaag cggtagccct gctggctgta cgcgatccgt tgcgtagtga tagcgtggcg
     4621 gcgctgcaac gtctgcataa agcgggatat cgtctggtga tgttgaccgg ggataaccca
     4681 accaccgcca atgcgatcgc caaagaagca gggattgatg aggtgatcgc cggggtgctg
     4741 ccggacggta aagccgaagc gattaaacgc ctgcaaagtg aaggacgtca ggtggcaatg
     4801 gtgggcgacg gcattaacga tgcgccagcg ctggcccagg cggatgtcgg cattgcaatg
     4861 ggcggcggca gtgatgttgc cattgaaacc gcggcgatta ccctgatgcg ccatagcctg
     4921 atgggcgttg cggatgcgct cgctatttcc cgcgcaacgc tgcgcaacat gaagcagaac
     4981 ctgctcggcg cgtttatcta caacagtatc ggtattccgg tcgccgccgg tattttgtgg
     5041 ccgtttactg gaacactgct taacccggta gttgccggag cggcaatggc gctctcgtcg
     5101 attaccgtag tgagtaacgc caaccggttg ctgcggttta aaccgaagga ataagtacgt
     5161 cattgcggat gcgctgcttt agggtggcgc atttggcatc gacgcatgac tgccggatgc
     5221 ggcgtaaacg ccttatccgg cctacggttc aggcacaggt ttgtaggcct gataagacgc
     5281 gtaagcgtcg catcaggcaa tggtgctcgg atgccggatg cggcgtgaac gccttatccg
     5341 gcctacgaga tgaagcgatg ttttggcctt acgcgacctt ttttgcacgc tcttcacggc
     5401 gtgccaaata ttcagccact gtttcaatcc gtcccaattc ttcctgggtg cgcatgttgt
     5461 tttcaacttc ccaaagatca actgccgcct ggaggtttag ccaaaaatcg actgtggtat
     5521 caaaaacttt cgccagacgg aatgccatct cagtggtgag tttacgattg ttattgatca
     5581 gtgcactgac gctattacga tgaacatgca gcaactctgc taactcattg attttcaaat
     5641 ctagcggttc cagatattca tagagaagaa tatctccggg tgtcgtcggt tttcttgttg
     5701 cctgtttcat agtttttaac tgttgcttcc ctgtaaatag cgatacaaga acatactgaa
     5761 tcatacaaac acattaaaca agtccccaac tccatttgcg ccatccctcc ccgcgcgcta
     5821 gcatgatatt tcacaaaggg aggtcgtatg gatctgttgt accgggtaaa aacgctttgg
     5881 gccgcgctgc gcggtaatca ttacacctgg cctgccattg atatctctct tcccggcaat
     5941 cgccattttc atctgattgg cagtattcat atgggtagcc acgatatggc tcccctaccc
     6001 acccgtttgc tcaaaaagct caaaaacgcc gatgcgctga tcgtcgaggc ggatgtttcc
     6061 accagcgata cgccttttgc taatttgcct gcctgcgagg cgctggaaga gcgcattagc
     6121 gaggagcaac tccaaaaact gcagcacatt agccaggaga tgggcatttc tccctcactc
     6181 ttttctaccc aaccgctgtg gcaaatcgcg atggttcttc aggcgacgca ggcacaaaaa
     6241 ctggggctgc gggcagaata cggtatcgat tatcagctat tgcaggcggc gaagcaacaa
     6301 cataaacccg tgattgaact ggaaggggcg gaaaaccaga ttgccatgtt gctccagctc
     6361 cctgacaaag gactggcgct gctggacgat acgctgaccc actggcatac caacgcacgg
     6421 ttgctgcaac aaatgatgag ctggtggctg aatgcgccgc cgcaaaataa tgaaataacg
     6481 ctgcccaata cgttcagtca gtcgctgtac gatgtgctga tgcatcagcg aaatctcgcc
     6541 tggcgggata aattacgcgc catgccgccg gggcgatatg tggtcgcggt cggtgcacta
     6601 cacctgtatg gagaagggaa tttaccgcaa atgttgcgct aaaaaaatgg ccaatatcgc
     6661 tattggcccg tcaaagagga atttcatttt tttattatta tgccgtcact ttaagcgacg
     6721 gtgtaactcg attgtcgctc aatctcacca tcctgccaat actcttaggc aagatgactc
     6781 ttttttttgg accgccatca ggcgtatgct tgcttcgttt ttttgtttaa ggcaggaaaa
     6841 ttaccatgac acccgcagtt aaatcactcg aaaaaaacaa gatttcgttt caaatccata
     6901 cctacgagca cgatccggct gaaaccaatt ttggcgatga agtcgtcaaa aaattaggtt
     6961 tgaatccgga tcaggtctac aaaacgctgc tggtcgcagt gaacggtgat atgaaacacc
     7021 ttgccgtggc cgttacgccg gtcgcaggtc aactggacct taaaaaagta gcaaaagcgc
     7081 tgggtgccaa gaaagttgag atggccgatc cgatggtcgc gcagcgttcg acgggatacc
     7141 tggttggggg gattagccca ctggggcaga aaaaacgtct gccaacgatt atcgacgccc
     7201 ccgcacaaga atttgccact atttatgttt ccggcggcaa gcgcggactg gatatcgaac
     7261 tggcggcagg cgatctggcg aagatcctcg atgccaaatt tgctgatatc gcccgccgcg
     7321 attaagcatt gtgccggatg cgaacatccg gcactttcgg attactgcca gctcacctca
     7381 cctttcggtt cataaacact cacatccagc ggcgagcttt tctggatata cgctttcagc
     7441 acttcggcat caataaagcc ggtattcaca tagcccggtt tgttatcaag gcgcggatat
     7501 ccatcaccgc cggtggcatt gaagtttaat gtcgccatac ggtaagtttt cgctggatcg
     7561 accggttcgc ctttgatttt aaggtcgttc agtttgccgt ctttcgccac aaagctaacg
     7621 ttggcaaatt gcgggtaggc acctgaatct ggcttcatct gcgcgacggc ggtcaggtaa
     7681 tcaatcacct ctttaccggt catgtcggca tacaccacca cattgccgaa tggctgcact
     7741 ttcagcacgt ttttatagct gatatcgcct gcttcgatag aatcacgaat tccgcctccg
     7801 ctcatcaccg caaagtcggc accagtgcga tccatttgtg ctgccagaat caaccgcccc
     7861 atattggtct gtacaaaacg cactttgtca cgatcgcctt ccagacgacc attggtttcg
     7921 cctattttca cttccagctg cgctttgcct ttgttctgga acggcgataa cagcgagatc
     7981 atttgctggt tttcagcgat ttcaggggtg tacagcacgc gctcgctttt cccgtcttcc
     8041 caggtcactt tcttcttcag gttcaccgga atcagctggt agttaaccat tttcatttcg
     8101 ccgttgcgaa actcaaagtc ggcccgacca acgtacttgc cccactcatg cgcctgtaca
     8161 atccagatgc cgttttgttg atccggtttg catggcgtac ctggaacgta atctacctgc
     8221 tttttatttt ccgctgccat acagactgga tcttgcgagt gaccgccgac aatcatcgcc
     8281 agcgatccgg caggcaacgc gcgtgccatc tccacatcgc ccggtgcgtt agaaccgtga
     8341 tcgccgttat cgtaatgccc catatgtgtc gccgcgataa taatgtctgg cttttctgtc
     8401 tgttgcagct cctgaatcac cagcttcgct tcatcggcgg gcttacggaa ctcaatatcg
     8461 gtgaagtttt ctggattgcc taacttcgct gtgtcatccg tcgtcagacc aataacggca
     8521 attttcagat cctgacgctt aaacagcgcc catggtttaa acaggcgctc gccggtactt
     8581 ttctggtaga tattggcaga aagtaacggg aactttgccc acttttcctg ctggcgtaat
     8641 acggtgagcg ggttatcaaa ttcatgatta ccgatcgcca tcgcgtcata gcccaccaga
     8701 ttcataccgc gaaaatcagg ttcggcatcc tgtaagtcag actcgggcac gccagtgtta
     8761 atgtcgccac cggaaagtag cagcacgcta ccgccttcag ccgcaacctc tttgcggata
     8821 ccatccacca gcgttttttg cgccgccaga ccatattcgc catattcatt gcgccaaaaa
     8881 tgcccatgat gatcattggt atgcagaact gtaattttgt aggttttatc ctgctcatac
     8941 gccagagcag cttcactcgc cagtgtaaat gtggttaaca gtgctaacgc cacgccccgc
     9001 tgcaataatt tcatacttct ctccctgacc tgatttcaac gtgttgaaaa atagtatgac
     9061 gcgacatcat agacaagtct gcgtccggtg aagataaatc tgcatccaga ttagaaattc
     9121 tgtattcagc gttgagactt ccttcaggcg gcgcatacag gtatgttagt cgggtaataa
     9181 tcacaaataa gatcattctt aacatcgcaa ccctgaaaaa gtgactttcc tatggcaatg
     9241 agtgaacaaa cccagcctgt ggcgggcgcg gctgcgtcaa cgaccaaggc ccgaacatcg
     9301 tttggtattt taggtgctat cagcctctca catctgctga acgacatgat ccaatcgctg
     9361 attctggcga tttatcctct gcttcagtca gaattttctc tgacatttat gcagattggc
     9421 atgataaccc tcaccttcca gctcgcctct tcgctactgc aaccagtggt cggctactgg
     9481 accgataaat atccgatgcc gtggtcgttg ccaattggca tgtgctttac cttaagtggt
     9541 ctggtgctgc ttgcgctggc gggcagtttt ggcgcagttc tgctggcggc ggcgctggtc
     9601 ggtaccggtt catcggtctt tcatccggaa tcttctcgcg tggcccgtat ggcttccggc
     9661 gggcggcatg gcctggcaca atctatcttt caggtcggcg gcaactttgg cagttccctg
     9721 ggacccttgc tggcggcggt gattatcgcg ccttatggca aaggcaacgt tgcctggttt
     9781 gtgcttgcgg cactgctggc gatcgtggtg ttggcgcaaa tcagccgttg gtactcggca
     9841 cagcaccgaa tgaataaagg aaaacccaaa gcgacgatta tcaatccact gccgcgcaac
     9901 aaagtggtac tggcggtcag cattctgtta atcctcattt tctcgaaata tttctatatg
     9961 gcgagcatca gcagctatta caccttttat ctgatgcaaa aattcggatt atctatccag
    10021 aatgctcagc ttcatctgtt tgccttcctg tttgccgttg cggcaggtac ggtgatcggc
    10081 gggcctgtag gggataaaat tgggcggaaa tatgtgattt ggggctctat cctcggcgtt
    10141 gcgccgttta cgctgatttt accctacgcc agcctgcact ggacgggggt tttaacggtg
    10201 attattggat ttatcctcgc ttcggcattc tctgccattc tggtctacgc tcaggagctg
    10261 cttccaggac gtatcggtat ggtttctgga ctctttttcg gttttgcttt cggcatggga
    10321 ggtctgggag cggcagttct ggggcttatc gccgatcaca ccagcatcga gttagtctat
    10381 aaaatatgtg ctttcctgcc actattgggg atgttgacca tattcctgcc tgataaccgg
    10441 cataaagact gatttcattg cagccaaagg caaactttgg ctgcatcgtt ttacagtcgc
    10501 cataagcctt tcctctgtta aaccgccttc tgttttccga tccgtgcttt tttgcagcta
    10561 attccatttt tctgcaaaat tcaataatta ttcatgaaca tcatcaatga aattgtcata
    10621 aactattacc tgtaattttg gttttcccgg ccaaaaatgg acaataacca ccaggaaaag
    10681 gagacggaat gcatcacgcc accccgctta tcaccaccat tgttggcggc cttgtgctcg
    10741 cctttatcct cggcatgctg gccaataaac tacgtatttc tcctctggtg ggatatctgt
    10801 tagcgggtgt gctggcagga ccattcactc cgggctttgt tgccgatacc aagcttgcgc
    10861 cggaactggc tgaactgggc gtcattctgt tgatgtttgg cgtgggtctg cacttttcgc
    10921 tcaaggattt gatggcggta aaggccatcg ccattcccgg tgcgatcgcc cagatagccg
    10981 tggcgacgct gctgggtatg gcgctctctg ccgtgctggg ctggtcgtta atgaccggta
    11041 tcgtgttcgg tttatgtctt tccaccgcca gtaccgtggt gttactgcgc gcacttgaag
    11101 aacggcaatt aattgacagt cagcgtgggc aaatcgccat cggttggttg attgtggaag
    11161 acctggtaat ggttctgacg ctggtgttgc tgcccgcagt ggcaggaatg atggaacagg
    11221 gcgatgtggg ctttgccact cttgcagtcg atatggggat caccatcggc aaagtgatcg
    11281 cgtttatcgc cattatgatg ctggtaggtc gccgtctggt gccgtggatt atggcacgca
    11341 gcgcggcaac cggttctcgc gagctgttta ccctgtcggt gctggcgctg gcgttaggga
    11401 ttgcctttgg tgcggtagag ctgtttgatg tctcctttgc actcggtgcg ttctttgccg
    11461 ggatggtact gaacgagtct gaactgagtc accgtgccgc ccacgatacg ctgccattgc
    11521 gcgacgcgtt tgcggtgctg ttttttgtct ccgtcgggat gttgtttgat ccgttaattc
    11581 tgattcagca accgctggca gtgctggcga cgctggcgat tattctgttt ggtaagtcgt
    11641 tagccgcgtt tttcctggtg cgactgtttg gtcactccca acgtacggca ttaactatcg
    11701 ccgccagcct ggcgcagatt ggtgagttcg cgtttatcct ggcgggactg ggaatggcat
    11761 tgaatttact gccgcaggcc ggacaaaacc tggtactggc aggggcgatc ctgtcgatta
    11821 tgctcaaccc agtactgttc gcactactgg agaaatatct ggcgaagacc gaaacgctgg
    11881 aagagcagac gctggaagag gcaatcgaag aagagaagca gatcccagtg gatatttgca
    11941 accatgcgct attggtgggt tacggtcgtg taggcagcct gctgggggag aaattgctcg
    12001 cctctgatat tccgctggtg gtgattgaga cgtcacgaac ccgtgttgat gagctgcgag
    12061 agcgcggggt ccgcgcagta ttgggcaatg cggcgaacga agaaattatg caactggcgc
    12121 atctggaatg tgcaaaatgg ctgatcctga cgatccccaa cggttatgaa gcgggtgaga
    12181 ttgtggcatc tgcccgcgcg aaaaatccgg atattgagat tattgcccgc gcccattatg
    12241 acgatgaagt ggcgtatatc accgaacgtg gtgcgaatca agtagtgatg ggcgagcgtg
    12301 aaattgcccg tactatgctg gaactgctgg aaacgccacc ggcgggtgaa gtggtgacgg
    12361 ggtaaacggt ggttgccgga tgtaaaatat gaaaaatatg taggcatgat aagacgcgcc
    12421 agcgtcgcat caggcattga gagcaaaatg ccagatgcgg cgtgaacgcc ttatctggcc
    12481 tacgaaccga cgataactta acgatcccag taagactctt ccaggctgtc ttcacgctcc
    12541 ggcaagccgc gcgttaaacg aggtgaatgc tggttcagta cctgatagct cacacggtta
    12601 gcatatttac acacctgcgc taacgatgaa taagttaacc aggtgaattt atgtttgctg
    12661 gagtttggta cgttgctacg atggtagctg ttggcggtaa tgtcatgcag caacgctgcc
    12721 agtgcgccat cccctgctcc gttggtgttc atgatttttt ccggcccgcc catgtacggc
    12781 gcaatgtgcg aatagacacg cagcggattc tgacaatcct tgtggcgcat ggcgcggcta
    12841 aactcatact ggttgaattc ggctatagcg cccggcagca acggatgctg ggttttacgt
    12901 ttcgcttcgt cttcggtaaa gcccgccata tacaagccga ttggcccggc ggtgcacagc
    12961 accagatcta cccagtccag cgccttgtca gatgccaaca acggatcgct ttctccggtc
    13021 aacgcttcgg cttcatcttc gttcatcgca aggatagaga cgtggtcttt gaggaattgc
    13081 tgccaccact gtggattctc ggcaatgaca aacttagttc ccagcgtcag caccaccggt
    13141 acgttatatt tcttcgcgta ctcaatggct ttcatggttg cttccggcat gggttcaccc
    13201 ggcttgcaac gcaccagata agaggtgaga accagagccg aggctccggc aatcacatct
    13261 tccggaatac tttcagcccg cagctggttc atgtggccag ggctgatagc aaaggtacgt
    13321 tccccggact cgccaatcag cgtaaagcaa cgaccaatcg ggccatccac gccttgtaga
    13381 tagttaagat cggtacggct ggaggtgtta cacaggtaac gataggcata gctgccaatt
    13441 tcaatattgc tgcacatgac gcccagcagc accgaacggt cgtccgcgag caccgagtag
    13501 ttatgcatgg tgttaccaat ggtgccaccc gcaaactgat gggtaatcag gtttttctgt
    13561 tttagttcct gataaagcgc ttcggctaca tcatcctcaa tcaccagtga atgcccggcg
    13621 cttaatccat aacgctcaat gaattcatca tccactttcg cttcaatatc gaccagcgtt
    13681 tgatcgatac ccactaccca ggcggcgctg gtttcgtttt ctggctggaa ttgctgaagc
    13741 agcggatcgc gtgcatttac ggggaagtaa tgtttggatt tacgtttacc gggaaatttc
    13801 atggttgttt ttacgggtgg ttaactgagc ggcgaatgtt aacacaatct gacgggagcg
    13861 cgcgagacgc agccagtttc aatatcagca ggcgggatcg cattttcatg gcagtggttc
    13921 cttacaatga cgtaatttga aaggagtttt tgtatgaagc cggaaaacaa attacctgtt
    13981 ctggacctta tttctgctga aatgaagacc gttgtgaata ctcttcagcc ggatttaccg
    14041 ccctggcccg caacgggaac gattgctgag caacgacagt attacacgct tgagcgccga
    14101 ttctggaatg cgggcgctcc agaaatggca accaaagctt acatggttcc aacaaaatat
    14161 gggcaggtgg aaacacgtct cttttgtccg cagccagata gcccggcaac gctattttat
    14221 ttgcatggag gcggttttat tctcggcaat ctcgataccc acgatcgcat catgcgcctg
    14281 ctggcaagct acagccaatg tacggtgatt ggtattgatt acaccctttc acctgaagcg
    14341 cgttttccgc aagtgataga ggaaattgtg gctgcttgtt gttatttcca ccagcaggcg
    14401 gaggattatc aaatcaatat gtcccgcatt ggctttgccg gtgattccgc aggtgccatg
    14461 ctggcgctcg ccagtgcgtt gtggttgcgt gataaacaga tcgattgcgg taaagttgcg
    14521 ggcgttttgc tgtggtatgg gctttacgga ttacgggatt ccgtgactcg tcgtctgttg
    14581 ggcggtgtct gggatggctt aacgcaacag gatttgcaga tgtatgaaga ggcgtattta
    14641 agcaacgacg cggaccgcga gtcgccgtat tactgtctgt ttaataatga tctcactcgc
    14701 gaagttccgc cctgttttat tgccggggcg gagttcgatc cgctgctgga tgacagccgt
    14761 ctgctttacc agacgttagc ggcgcatcag cagccctgtg agttcaaact ctacccaggc
    14821 acgctgcacg cctttttgca ttattcacgg atgatgaaaa ccgccgacga ggctcttcgc
    14881 gacggcgctc agttctttac cgctcagctt tagcgatacg cggcaacaag attagccatc
    14941 atttcgatat gttccggtgt ggcattaagc gccggaatat attcatattt tttcccgccg
    15001 gcaccgagga agacctcacg gttttgctcg gcaatctctt ccagcgtctc cagacaatcc
    15061 gcagcaaagc ccgggcacat cacctgaata tgacctacgc ctttttctcc gagcattttc
    15121 agcgtttcgt cggtataagg catcagccag ggttcccgac caaagcgcga ctgaaaggtc
    15181 atcatcactt tttccggtgc catccctaat gcggaagcca gttcacgagt cgttgtgcgg
    15241 caacgttgcg ggtaatcatc gccttcatct gcataacgct ggggaatgcc atgataagag
    15301 agcagcagca gatccggttc gccatgtttg gcaaaagaag cgcgtacgct gttcgccagt
    15361 gcattaatgt aatcgtggtt atcggcgtaa tcacgtataa acgatatccc cggaatgcta
    15421 cgtttgcgcg ccagaatgcg tgccagttca tcccataccg caccgaccgt agaacaggag
    15481 aattgcggat aaagcggcag caccacaata tgatctacat gctctgccag gagttcatct
    15541 acggcgcttt ccagtgatgg cgagccgtag ctcattccca gcgctacggg cgtctccggt
    15601 aaacgttgtg ccagcgcctg ctgttgctgg cggctgtaaa ccatcagcgg cgagccacct
    15661 tccatccaga cagaggcata cagcttcgcc acacgcggcg agcgcagcgg caaaatcacg
    15721 ccgcgcagca atggccacca taacaaccgt gaggtatcaa ccacgcgtct gtcgcttaaa
    15781 aattgtttca gatagcgttt taccgcttca ggtgtggggg catcgggcgt acccaggttt
    15841 gccagcagga taccggtttt agtctgacgc attaccgcct cttatcgatt caacttgttg
    15901 ataattgtag cggaaaagtg aggaagaaga actaattgct gtgagaggta ttgcctgagg
    15961 tggatgagca gacctgcccg aaagcagatc tgctgcggct tgattagccg aggatttttt
    16021 ccagatcagc gcgaacttca gcaaccggct tggtgccgtc aactttcgcg tatttggtgt
    16081 tacccgcttc cgcttctttg gagtagtagc cgatcagcgg tgctgtcatc tgatggtatt
    16141 caaccagacg tttacgtacg gtttcttcct gatcgtcttt acgggtagtc agttcttcac
    16201 cggtaacgtc gtctttacct tctactttcg gcggattgaa tttaacgtga taaacacgac
    16261 cagacggcgc gtgaacgcgg cgaccgacga tacggtcaac gatcagttcg tccggtacgt
    16321 cgaattccag aacgtaatca acattgatgc ccgcttcttt catcgcgtct gcctgcggaa
    16381 tggtacgcgg gaagccgtcc aacaggaaac cattacggca gtcttcctga gcaatgcgct
    16441 ctttaaccag cgcgatcacc agttcgtcgg tgaccagttt gccagcatcc ataatgtctt
    16501 ttgcttgttt acccagctcg gagccagatt tgaccgcagc acgcagcata tcgccagtgg
    16561 agatttgcgg aataccatat ttctccatga tgaactgagc ctgagtccct ttccccgcgc
    16621 ccggagcgcc aagcagaatg atacgcattg cgaaaatccc cttaaaatgt gtcgaatttt
    16681 ttgaaaaagc gataaacgat accaccatca ggcgattgcc tcaagaaagg caccaggggc
    16741 tgaaacggtt aatggcgaga taatttgcgg aaagtgcaga taatgaaaag aaaaatgccg
    16801 gatgacacga aggtcatccg gcattacatc aggaaaccag cagctggttc atacgacgaa
    16861 taaacaggtt cggatcttcc agcgtgccgc gttctgccag cagtgcctga tccagcagca
    16921 gttctaccca ctcgctgaac ttcgcttcat cttcagtatc tgccgcacgt ttcaccagta
    16981 cgtgatccgg gttcagttcg aagatgtatt tcacttctgg cactttctgg cccgccgcag
    17041 cgaacagttt cgccatctga gtgctcattt cgtccgcgtc ggtcgaaacg atcgctggcg
    17101 tatcggtcag acggtgagtc agacggacat ctttcacgcg ctcgccgagc agggctttca
    17161 cacggtcgat gaacggagtc agtgctttct ccgcttcttt cgcgctctca tcaacttcgt
    17221 cagccagttt ttccagcgac tcgtcaactt tagacaccga ctggaacggt ttaccgtcga
    17281 actcagtcag atagttcatc atccactcat cgatgcggtc ggaaagcagc agaacttcga
    17341 tgcctttctt acgcagcagt tccaggtgcg ggctgctctt cgctgccgca tagctgtctg
    17401 cagtgatgta gtagattttc tcctgccctt ctttcatgcg ggaaacgtag tcttccagag
    17461 acacggtctg cgcagaagaa tcggtatggg tagaagcaaa acgcagcaat ttggcgatcg
    17521 cttcctggtt agcgaaatct tccgccggac cttctttcag taccaggcca aactgttgcc
    17581 agaaggtctg gtatttttcc gcgtcgtctt tcgccagttt ttccagcatt tgcagcacac
    17641 gcttggtcag cgcattgcgc aggttacgcg ttaccgtgct gtcctggagg atttcacggg
    17701 aaacgttcag cggcagatcg ctggagtcaa tcagaccacg cacgaagcgc agatagttcg
    17761 gcatgaactg ttctgcgtcg tccatgatga acacacgctg aacatacagt ttcaggccgt
    17821 gtttatgatc gcggttccac atatcccacg gagcctggga cggaatgtac agcaggctgg
    17881 tgtactcctg cttaccttca acacggttgt ggctccaggt cagcggatcg ttaaaatcgt
    17941 gggcgatgtg tttgtagaac tctttgtact cttcatcggt gatttccgac ttgttacgag
    18001 tccacagcgc ctgcgctttg ttaattttct cccaggagat aacggtttcg ccgtctttct
    18061 cttcgcgttt ttcgatctct accggcagcg cgatatggtc ggagtatttg ctgatgatgg
    18121 aacgcacgcg ccagtcatcg aggaactcgt cttcgccttc acgcagatgc agggtgattt
    18181 cagtaccacg atcttctttg gtgatgtcgg caacggtata ttcaccttcg ccagccgatt
    18241 cccagaagac gccgttttct ggtttttcgc ctgccgcgcg agtacgcacg gtcactttgt
    18301 cggccacgat aaacgcagag tagaaaccaa caccaaactg accgatcagc tggctgtctt
    18361 tcgcctggtc agaacccagg gattcgagga atgatttggt accggattta gcgatagtcc
    18421 ccagatggtc aatcacttcg tcgcgggtca tccccacgcc gttatcggag atggtcagcg
    18481 tgcgcttgtc tttatcgaaa gagacacgaa cgcgcagttc gccatcacct tcgtacaggt
    18541 ccgggttaga gagcgcacgg aaacgcaact tgtccgccgc atcggaggcg ttagagataa
    18601 gctcacgcag gaagatttct ttattggaat agagagaatg gatcatcagg tgcagaagct
    18661 gtttcacttc tgactgaaaa ccacgagttt cttgtccttt catgtaggtc tacctcaata
    18721 atgccatttt aaggttaaaa acaggatgag ggagagatgg ggacaaggga gaataatttc
    18781 aagcgagagc aggtgatcct gctctcgttt gcttaaaaac gaatcttatg acgcccggca
    18841 agggagtgtg acagcgtggt gccatcgacc atttccagtt cgccgccaac cggtacgcca
    18901 tgagcgattc ggctggcttc cacgtcatat tgcgcgcaaa gctcggcaat gtagttagcg
    18961 gtagcttcac cttcaaccgt agggttggtg gcgaggatca cttcagtgat tttttcctct
    19021 gccagacgct gttccagacg atcaaggccg atatcatccg gaccgatgcc gtccagcggt
    19081 gacagatgtc ccatcaacac aaaataacga cctgaaaact gccccgtctg ctcaatggcg
    19141 taaatgtccg ccggactctc caccacgcag atttgaccgt tttcctgacg acgcggattc
    19201 gaacagatgt tacagacttc ctgttcggtg aaggtgcggc aatcggcgca gtggccgatt
    19261 tccgacatcg cccgggtgag cgcctgcgcc agacgcatcc cgccgctacg atcgcgctga
    19321 agcagtgtga acgccatacg ctgcgccgac ttcgggccaa cgcccggcag acagcgcagt
    19381 gcttccataa gctgtgttaa cagcgggctg gtttgcatca gaacggcatc ttaaagccag
    19441 gcggcagctg cattccggag gatacagaag ccattttttc tttctgcgtt tcttcaatac
    19501 gacgtgctgc gtcgttgaat gctgcagcca ccaggtcttc cagcatctct ttgtcgtctt
    19561 ccagcaggct cgggtcgatc tctacgcgac ggcagttatg tgcaccgttg atggtcactt
    19621 ttaccagacc tgcgccagat tcgccggtaa cttccagctg cgcgatctct tcctgcattt
    19681 tctgcatttt ttcttgcatc tgctgggctt gcttcatcag gttacccaga ccgcctttac
    19741 caaacatagg tttctctctc aatcacgtta aggatgacga acgtaagctg tgcttacgat
    19801 caaatggggc ggatactgtc ttcatccagc tccgcatcga agaaccgacg cagggtctga
    19861 atattattat ccgcaataat ggactcgcgc gcctgcgcaa gtttttcttc gtatatcgcc
    19921 tgacgccact ccagcggcgt acgcaccgcg ggattatcat cttcaactat agtcagttca
    19981 accgttgaac cttttaacat gctcaacgct tcagccagtt tttgctgtgc accgcggttg
    20041 ttcaaatgcc gctgagagga gcgcaaatgc agacatactg cgttgtcgct ctcctctttc
    20101 caggcattta acgccacctg ttcgaccagt tttggtagcg aaagttgact cacctgagcc
    20161 gcccacgcgt cgcgctcaat ggcttctgcc gccagcttcg ccgccagttc cggcgttttt
    20221 tcatgttcca gcgctttttt cagcgccttc ggtgtggcga ccacttcttt ttgctgcatc
    20281 actggagtgg tcgccttcca gcgatacgct tcttttttag ctggcgcttt ttccagcgcc
    20341 gatggcaccg gacgcgcctg aacgcgatcg gtgaccgaag ccagtctttc cagcgcagcg
    20401 ttattcaccg gccgcgcgcg ggtagcggct gccggttcac tcttttttgc tttggttgct
    20461 ccctgcacgc gctgcaactg ctggcgcgcc gccagcacct ggctggtggt ttccgggagc
    20521 ggtacagtcg gtgcctgctg cggcgctgat tgcggttgcg gcggcacctg ggttggcgtc
    20581 attactgccg ttggcgcgac gggtgcaaag gactggcgtg gcacttctgg ctcaggcagc
    20641 ggcatacgcg gatggaacgc cagcgcgcgc agcagcgtca tctcaacgcc catgcggcgg
    20701 tccggcgcat acggtaattc tttgcgacca atcaacagcg tctgatagta aagctgaata
    20761 tccgtcggcg gtatggtgcg cgccagttca cgcatccgca gctcgatggc ggccatgtcg
    20821 ttgccaagtg cagcaggcga aagttgtacc atcgcaatac gatgcaacag gccgagcatt
    20881 tccaccagca acgcttccca ctcgatacca cgggcagcgg cttcattaat cagcgccatt
    20941 acgcgctcgc cgttggcctc gaccatcgct tcaaccagcg acagcgcctg atcgtcgtca
    21001 agcgtaccca gcatcgcact gaccgcctgg gttgaaacct ggccgtcacc gctggcaatc
    21061 gcctggtcgg tcagacttaa ggcatctcgc aggctgcctt cagcggcgcg cgccagcaat
    21121 tgcagcgccc gcggctcgtg agcgatatgt tcttcattga ggatgtgctc aagttgatga
    21181 cgaatttgct cgacatccag cgccttgaga tgaaattgca aacagcgcga caaaatcgtc
    21241 accggcaatt tctgtggatc ggtcgtcgcc agcagaaact taacgtgctc cggcggctct
    21301 tcaagggttt ttaacagtgc gttaaagctg tggcgcgaca gcatatgcac ttcgtcgatg
    21361 agataaactt tgaaacgacc acgcgccgga gcgtactgga cgttatccag caggtcgcgg
    21421 gtatcttcaa ctttggtgcg cgaggcggcg tcgatttcaa tcagatcgac aaagcgcccc
    21481 tgctcgattt cacgacagtt atcgcacacg ccgcacggcg tcgcggtaat gccggtttcg
    21541 cagtttagcc ccttcgccag cagtcgggcg atagaggttt ttccgacgcc acgggtgccg
    21601 gaaaaaagat aagcatgatg aatacgccct aacgacaagc cgttcgccag tgcggtcagc
    21661 acatgttcct ggccgacgac gtcagcaaag gtttgtgggc gccattttcg ggctaagacc
    21721 tgataactca ttggcaggct ctgaaacgct ggaaggtgga ttctcgaagg ggtaatgcta
    21781 acacagcccc gtcagaacgg cgaggctgaa tgcacatctt gtgctgtccg tagcgtgggc
    21841 agcacaagac tggcgataat taatggcccg ggaacgggac aaggctgtag ctggtaatgc
    21901 cctgtttttc gagacgctgt tcgccgccga gatcgaacag gttgataatg aacgcagcgt
    21961 cagccacttc accacccaga cgacggatca gtttaacggt cgcttcgata gtgccgccgg
    22021 ttgccagcag atcgtccacc accagaactt tgtcgcccgg tttgatggca tcaacgtgga
    22081 tctccagctg atcggtgccg tattccagat cgtaagtttc actgatggtt tcacgcggca
    22141 gtttgcccgg tttacggacc ggtacaaagc caacgcccag acccagagct accggagcgc
    22201 caaacaagaa gccacgcgct tcggtgccga caactttgtt aatgcccgca tttttgtaac
    22261 gctcaaccag caagtcgatg ctgagagcgt aagctttcgg gtcttccagt aagctggtga
    22321 catcgcggaa aagaatgccg ggttttgggt agtcctgaat gcttttgatg ctatttttga
    22381 gatactcaag ctgctgtgca gtcgcggtca taagtgtatg cctgcttgtt acggtggtac
    22441 tcacggcgcg ttttaaacgt atcaaaagta acagttgttt aacctttgac cgcctgcgcc
    22501 tgtgctcgaa aacgccagaa tgtactggct gtacgcaata attgcaactg cgattgttgt
    22561 gcttcagtgc ttttcttgct tttcatcaat caccggaatt cgccacatat agaaaagcaa
    22621 acaggcgaga attaccagca acatgatgcg cacccatggc atctggacga accacagaga
    22681 aatggcaaac gtgagcaaaa taagcaaaat cgcccgcggt ttgacgccgc gcggcatcgc
    22741 atgatgtttc tgccagaaac gtagatagct gccaaaccat gagcggtaca gcaaccaggc
    22801 gtgaaagcac ggggaagaac gggcaaagca ccaggccgcc agcaggataa acggcgtcgt
    22861 cggtaatacc ggtaatacca cgcccagcgt gcccagcact accgccagcc agccaatgat
    22921 gattaaaatg attcgttgca tattgagtgt tgtcagctta cagagtggct actttagcat
    22981 aacaattatc attttcattg aggtcttatc gtgaaaaccg ccctgctgct ggaaaaactg
    23041 gaaggacagc tcgctacgct gcgtcagcgt tgtgccccgg tgtcacagtt cgccacgcta
    23101 agtgctcgtt tcaacaggca tctttttcag actcgtgcga caacactaca ggcttgtctc
    23161 gacaaggcgg gcgataatct ggctgcgctt cgtcatgcag ttgagcaaca gcaactgccg
    23221 caagtcgcct ggctggcaga acatctggcg gcgcaactgg aggctatagc gcgtgaagcc
    23281 acagcctggt cgctacgcga gtgggatagc gcaccacccc aaattgcccg ctggcagcgt
    23341 aaacgtattc agcatcagga ttttgagcgg cggctacgtg agatggttgc cgaacgtaga
    23401 gcccgcctgg cgcgggtgac cgatctcgtg gaacagcaaa cgctgcatcg tgaagtggaa
    23461 gcctatgaag cgcgcctggc acgctgccgc catgcgctgg aaaaaatcga aaacaggtta
    23521 gcgcgtttaa cccgctagca atggagagag tatgtcactg gaaaatgccc ctgacgatgt
    23581 caaactggct gtcgatttga ttgtgctgct ggaagaaaat cagatcccag cccgcaccgt
    23641 gctgcgcgcg ctggatattg taaagcgtga ttatgaaaag aagttacaga gcgatgaggc
    23701 ttcacaaagc gagtaaaaaa taaagggcgg catcactgtc gccctttatt gtccttaccc
    23761 taccgctggc gtcggatcgt cgcctttgta gtcgcgtttt acttccgtca cctcatcgcc
    23821 cttctcgttg tgcagatgca cttcaagctg gttaaaggca atgttgatgt cgttttcacg
    23881 gcacagctga tcgatagtac ggttcagctc atcgacagta cgactacggt cacgcagttc
    23941 acgcacatac agacgcagct catgatccaa cgtgctggca ccaaatgccg taaagaagac
    24001 ttccggcatt ggttcgtgca tcacccttgg gtgctcagtc gccgccttca gtaacacttt
    24061 acgcactttt tccagatcgg agccataggc cacgccgaga cggatcacca gacgcgtagt
    24121 agtgtcagtc aacgaccagt tgatcagacg ctcggtaaca aacgctttgt tcgggatgat
    24181 cacttctttg cgatcgaaat cggtaatcgt tgtcgcacga atacggattt tacttaccgt
    24241 ccccgagaag ctaccaatgg ttaccgtatc gccaatacgc accggacgtt cgaacagaat
    24301 gatcaaaccg gagacgaagt taccgaaaat ttcttgtaaa ccaaaaccaa gacctacgga
    24361 taatgctgcg gccagccact ggagtttatc ccaagagacg cccagcgatc cgaacaccgt
    24421 catcgcacca acagcaataa tgatgtagtt aaggatggta gtaatggcat acgacgcgcc
    24481 ctggcgcata ttcagtcgcg agagcaccag cacttccagc aaaccaggca ggttgcgaat
    24541 caacgcccag gccaccattg aggcgataat cgcaaacaac agactaccca tggtgacgtt
    24601 tttcaccacc gcagcaccag cttcagtacc gttgtaatgc cacagcgtaa tgctgtcgag
    24661 atagctgaac acggtgatca aatcggacca aattgcccag aacatgacac cgaacagcgc
    24721 aaacatcagc aacatggtaa tacgcagcgt ctgctggtta acctgttcca gtgcaatggt
    24781 gggttcttcc ggcggttcgg caccttctgc gccctctttc accagattct gccgacgcgc
    24841 cagcgcacga cgccaggcga tacgccgcgc cgctacgctt aagccgcgca ataccgtctg
    24901 gtagagcaag ttccagatga tcaccagata aacggtttca atccaacgcc cggagaggcg
    24961 cagcgtggtg tagaaataac ccgtggcagt cagcaccatc aatgcaatcg ggataatcga
    25021 cagcacggta atggtgacca gtcgcatggt gtgcgactct ttatcacgcc agctttcgcg
    25081 gcacattggc cataccagga aggcaatcag cagcaggttg aagaaaatca tcgcctgccc
    25141 cagcacatca tccatcagat gcagcgggga aagttctgcc accacagacc agaaatggat
    25201 aggcagcaac gcgagactga tgcggacaat ttgccgacgc cagtggctgg tctgctgttc
    25261 cggcatgccg aagtgacgca cggcaacgcc gttcttctcc agcactttcc agcacaggcc
    25321 aaacaccagc cagaaaatcg ccagtttttt gctgaacgac catagcagtt cgctgatgtt
    25381 gagctgcatg gtcaacagaa tcaggccaac cgcgagaata atcaggcaca ccggcagtgc
    25441 acggatcagg tcgataagga tcgcttttgg tgtgttgagc tggctgtcgt tacgcaggga
    25501 acccacagcg gaagccagtt tttgttgata cgctttcagc caccccagac gccagtggat
    25561 cagcccggca atcaacagca gcggcaaacc agcgaggaaa gcgataaaaa cggcgggcca
    25621 ggctttttcc cagttaaccg taattttcat cgacttaaat tcatctttca ggctttgcgg
    25681 gaacgctttg atccagtccc agtccattgg acggttactg ttcacccaaa agatttgctg
    25741 agtcaggatg gatttcaggt ttttcgacac actcattaac tgctgctggt tgatttgcag
    25801 gttaatggcc atcatcagct ggttacccaa ctgtttgttg agttgatcca gcaattcgcg
    25861 acgcatatca accacttgca ataacgcatc gtgaacttcg ctgttgactt cgttggtgtg
    25921 accttcttcc agtttgttga cgaacgcatc gctctggaag agtgcatcac gctgctggtt
    25981 aacttcaaac tgttcgagac gcaaatccgc gatgcggttg gtcatgtttt ccagttcatc
    26041 cgccgagggc agcgtttgtt gttgctggta aaggatacga gacaacagca ggctgccctt
    26101 caggacggca atctgctctt taatattgcg ttccgattgc agcgcccgct ccagccagtt
    26161 tttgacttta atgttttgct gcatcaactg attaccgttt tcagtcgcgg taatcagacg
    26221 ctgacttaac tgctggttaa tttccagttc ctgcttcacc agcggattag cctgaatacg
    26281 cgcggcttca tccggggaga cggcttcctg cgccgttttt tcggttaaag tcaggcgctt
    26341 gctgtttacc gcttcttgca acagctgcaa ctggtgctcc agacgggcgc tgttcgccgt
    26401 cacgtaatca cgttgctttt gcaaggtatc ctgcaagacg gtattccctt ccaggctttt
    26461 acgctgctgg tcaatctccg cattcagcaa cgcctgctga gcctgcatta acactttctg
    26521 gctgggacgt aaggccgtct cgccgacatc agtcccatcc agacgactgc gaatttgttg
    26581 cagctgctgc gaagcgttat acatcgcatt ttgcacgcgt tcgggctgcg tctgtaacga
    26641 aaccagctgg ctgttataag acgccagatc gttttgtgcg ttttgcaaat cgtccagcgc
    26701 ctgggcaacg cgagtttcca gctggcgcaa cgaaagcgtg ctcagaattt tgcgcgtttc
    26761 ttcgtcgtta tcgacatcgc taagtgctgt taacgccgcg gtcgcctggc gcattttttc
    26821 cggcgcttca gcgacttttt gccgtagctg aacggtctct tcttttacgc gatcgatttt
    26881 atcgagggtg gctaatgtat ctgtcagatc ctgctgcacc agtttgtcct gagcagaaag
    26941 atctttttgt ttatttagtg agtcaagttg cgcctgcagg tccgcttttg tcggcagatc
    27001 accattcgat gacgcccgcg caaacgccgt gttctggcat aacaagacaa aaacaaaagc
    27061 aataaatgct gaaaaaacaa aatgccgtga tcgtttgtaa tactggaaca tagtcatgat
    27121 gaatgaaggt ttctgaacct gaagaacgac ctgaaaaagt caaaccgcaa gaatatcacg
    27181 acgcagtgaa ccagaatagc aacgacgaaa atgtccagga aaaatcctgg agtcagattc
    27241 agggttattc gttagtggca ggattacgaa gcgtggggca caggagatac atctccagta
    27301 agatggcgac gtaatcgcgg gcttcttttt taagatcaaa agattgcggg gcaaagagcc
    27361 agttttccat caggccggaa atatagccgc gcataataat tgctgcccga cgcgtcatta
    27421 aatccgcagg caacattttc gcttcaatac aatgttttaa cgtttgttct atacggtcat
    27481 aactttccaa acagagatta cgttgtgcct gttgcacaac agccatttct ccgacaaatt
    27541 cgcatttgtg gaatataatc tccatcaata atcgacgccg ttcttctgtc accgtggatt
    27601 caagaacatg aattaatatc tctcttaata ctgagagtgg atcgccaggg aattttgcct
    27661 gatactcaag ctctagttca ccaatattgg attctgatag ttcccagatc tcactgaaca
    27721 aatccgactt gtctttaaaa tgccagtaga ttgcaccgcg cgtaacgcca gctgcttttg
    27781 caatctcacc cagcgaggtg gatgataccc cctgctgtga gaaaagacgt agagccacat
    27841 cgaggatgtg ttggcgcgtt tcttgcgctt cttgtttggt ttttcgtgcc atatgttcgt
    27901 gaatttacag gcgttagatt tacatacatt tgtgaatgta tgtaccatag cacgacgata
    27961 atataaacgc agcaatgggt ttattaactt ttgaccattg accaatttga aatcggacac
    28021 tcgaggttta catatgaaca aaaacagagg gtttacgcct ctggcggtcg ttctgatgct
    28081 ctcaggcagc ttagccctaa caggatgtga cgacaaacag gcccaacaag gtggccagca
    28141 gatgcccgcc gttggcgtag taacagtcaa aactgaacct ctgcagatca caaccgagct
    28201 tccgggtcgc accagtgcct accggatcgc agaagttcgt cctcaagtta gcgggattat
    28261 cctgaagcgt aatttcaaag aaggtagcga catcgaagca ggtgtctctc tctatcagat
    28321 tgatcctgcg acctatcagg cgacatacga cagtgcgaaa ggtgatctgg cgaaagccca
    28381 ggctgcagcc aatatcgcgc aattgacggt gaatcgttat cagaaactgc tcggtactca
    28441 gtacatcagt aagcaagagt acgatcaggc tctggctgat gcgcaacagg cgaatgctgc
    28501 ggtaactgcg gcgaaagctg ccgttgaaac tgcgcggatc aatctggctt acaccaaagt
    28561 cacctctccg attagcggtc gcattggtaa gtcgaacgtg acggaaggcg cattggtaca
    28621 gaacggtcag gcgactgcgc tggcaaccgt gcagcaactt gatccgatct acgttgatgt
    28681 gacccagtcc agcaacgact tcctgcgcct gaaacaggaa ctggcgaatg gcacgctgaa
    28741 acaagagaac ggcaaagcca aagtgtcgct gatcaccagt gacggcatta agttcccgca
    28801 ggacggtacg ctggaattct ctgacgttac cgttgatcag accactgggt ctatcaccct
    28861 acgcgctatc ttcccgaacc cggatcacac tctgctgccg ggtatgttcg tgcgcgcacg
    28921 tctggaagaa gggcttaatc caaacgctat tttagtcccg caacagggcg taacccgtac
    28981 gccgcgtggc gatgccaccg tactggtggt tggcgcggat gacaaagtgg aaacccgtcc
    29041 gatcgttgca agccaggcta ttggcgataa gtggctggtg acagaaggtc tgaaagcagg
    29101 cgatcgcgta gtaataagtg ggctgcagaa agtgcgtcct ggtgtccagg taaaagcaca
    29161 agaagttacc gctgataata accagcaagc cgcaagcggt gctcagcctg aacagtccaa
    29221 gtcttaactt aaacaggagc cgttaagaca tgcctaattt ctttatcgat cgcccgattt
    29281 ttgcgtgggt gatcgccatt atcatcatgt tggcaggggg gctggcgatc ctcaaactgc
    29341 cggtggcgca atatcctacg attgcaccgc cggcagtaac gatctccgcc tcctaccccg
    29401 gcgctgatgc gaaaacagtg caggacacgg tgacacaggt tatcgaacag aatatgaacg
    29461 gtatcgataa cctgatgtac atgtcctcta acagtgactc cacgggtacc gtacagatca
    29521 ccctgacctt tgagtctggt actgatgcgg atatcgcgca ggttcaggtg cagaacaaac
    29581 tgcagctggc gatgccgttg ctgccgcaag aagttcagca gcaaggggtg agcattgaga
    29641 aatcatccag cagcttcctg atggttgtcg gcgttatcaa caccgatggc accatgacgc
    29701 aggaggatat ctccgactac gtggcggcga atatgaaaga tgccatcagc cgtacgtctg
    29761 gcgtgggtga cgttcagttg ttcggttcac agtacgcgat gcgtatctgg atgaacccga
    29821 atgaactgaa caaattccag ctaacgccgg ttgatgttat taccgccatc aaagcgcaga
    29881 acgcccaggt tgcggcgggc cagctcggtg gtacaccgcc ggtgaaaggc caacagctta
    29941 acgcctctat tattgctcag acgcgtctga cctctactga agagttcggc aaaatcctgc
    30001 tgaaagtgaa tcaggatggt tcccgcgtac tgctgcgtga tgtggcgaag attgagctgg
    30061 gtggtgagaa ctacgacatc atcgcagagt ttaacggcca accggcttcc ggtctgggga
    30121 tcaagctggc gaccggtgca aacgcgctgg ataccgctgc ggcaatccgt gctgaactgg
    30181 cgaagatgga accgttcttc ccgtcgggtc tgaaaattgt ttacccgtac gacaccacgc
    30241 cgttcgtgaa aatctctatt cacgaagtgg ttaaaacgct ggtcgaagcg atcatcctcg
    30301 tgttcctggt tatgtatctg ttcctgcaga acttccgcgc gacgttgatt ccgaccattg
    30361 ccgtaccggt ggtattgctc gggacctttg ccgtccttgc cgcctttggc ttctcgataa
    30421 acacgctaac aatgttcggg atggtgctcg ccatcggcct gttggtggat gacgctatcg
    30481 ttgtggtaga aaacgttgag cgtgtgatgg cggaagaagg tttgccgcca aaagaagcca
    30541 cccgtaagtc gatggggcag attcagggcg ctctggtcgg tatcgcgatg gtactgtcgg
    30601 cggtattcgt accgatggcc ttctttggcg gttctactgg ggcaatttat cgtcagttct
    30661 ctattaccat tgtttcagca atggcgctgt cggtactggt ggcgttgatc ctgactccag
    30721 ctctttgtgc caccatgctg aaaccgattg ccaaaggcga tcacggggaa ggtaaaaaag
    30781 gcttcttcgg ctggtttaac cgcatgttcg agaagagcac gcaccactac accgacagcg
    30841 taggcggtat tctgcgcagt acggggcgtt atctggtgct gtatctgatc atcgtggtcg
    30901 gcatggccta tctgtttgtg cgtctgccaa gctccttctt gccagatgag gaccagggcg
    30961 tatttatgac catggttcag ctgccagcag gtgcaacgca ggaacgtacg cagaaagtgc
    31021 tcaatgaggt aacgcattac tatctgacca aagaaaagaa caacgttgag tcggtgttcg
    31081 ccgttaacgg cttcggcttt gcgggacgtg gtcagaatac cggtattgcg ttcgtttcct
    31141 tgaaggactg ggccgatcgt ccgggtgaag aaaacaaagt tgaagcgatt accatgcgtg
    31201 caacacgcgc tttctcgcaa atcaaagatg cgatggtttt cgcctttaac ctgcccgcaa
    31261 tcgtggaact gggtaccgca accggctttg actttgagct gattgaccag gctggccttg
    31321 gtcacgaaaa actgactcag gcgcgtaacc agttgcttgc agaagcagcg aagcaccctg
    31381 atatgttgac cagcgtacgt ccaaacggtc tggaagatac cccgcagttt aagattgata
    31441 tcgaccagga aaaagcgcag gcgctgggtg tttctatcaa cgacattaac accactctgg
    31501 gcgctgcatg gggcggcagc tatgtgaacg actttatcga ccgcggtcgt gtgaagaaag
    31561 tttacgtcat gtcagaagcg aaataccgta tgctgccgga tgatatcggc gactggtatg
    31621 ttcgtgctgc tgatggtcag atggtgccat tctcggcgtt ctcctcttct cgttgggagt
    31681 acggttcgcc gcgtctggaa cgttacaacg gcctgccatc catggaaatc ttaggccagg
    31741 cggcaccggg taaaagtacc ggtgaagcaa tggagctgat ggaacaactg gcgagcaaac
    31801 tgcctaccgg tgttggctat gactggacgg ggatgtccta tcaggaacgt ctctccggca
    31861 accaggcacc ttcactgtac gcgatttcgt tgattgtcgt gttcctgtgt ctggcggcgc
    31921 tgtacgagag ctggtcgatt ccgttctccg ttatgctggt cgttccgctg ggggttatcg
    31981 gtgcgttgct ggctgccacc ttccgtggcc tgaccaatga cgtttacttc caggtaggcc
    32041 tgctcacaac cattgggttg tcggcgaaga acgcgatact tatcgtcgaa ttcgccaaag
    32101 acttgatgga taaagaaggt aaaggtctga ttgaagcgac gcttgatgcg gtgcggatgc
    32161 gtttacgtcc gatcctgatg acctcgctgg cgtttatcct cggcgttatg ccgctggtta
    32221 tcagtactgg tgctggttcc ggcgcgcaga acgcagtagg taccggtgta atgggcggga
    32281 tggtgaccgc aacggtactg gcaatcttct tcgttccggt attctttgtg gtggttcgcc
    32341 gccgctttag ccgcaagaat gaagatatcg agcacaacca tactgtcgat catcattgat
    32401 acaacgtgta atcactaagg ccgcgtaagc ggcctttttt atgcataacc tacgaacatt
    32461 aaggagtaat tgaaccacca actcaggatc tcatacgaaa accagtatta accacgtata
    32521 aaattcataa aaaatactga ttgttagtta atttatatta agtagcgcta atagatttaa
    32581 taatccataa tcatttagag gctattctta attatttgcg gtaattcttt attcattcct
    32641 cggttattac gtcatattca gagcaatcct ggtattagtg tcaccaattt catctggcga
    32701 taatcctgaa atgttatgaa cagttcgagc aaactgcttt tacctgctgc gggttggtgc
    32761 tagtatgaaa aagtgagccc tgtcccgctt ccttcctaat tgtaattttt cgtaataatg
    32821 cgatgaaaac ctgcaaagag tggcttatag ttaagctaac aaacgagagg gcaagtccag
    32881 gtcagtaagt tttttccatc ccgaaaggtg tccgttagtt caaccgctaa gaaggggacg
    32941 cgttatggat gaatactcac ccaaaagaca tgatatcgca cagcttaagt ttctctgtga
    33001 aactctgtat catgactgcc ttgcaaacct tgaagaaagc aatcatggct gggttaacga
    33061 cccaacctcg gcgatcaacc tccagttgaa tgaactgatt gagcatattg cgaccttcgc
    33121 acttaattac aaaattaagt ataatgaaga caataagctc attgagcaga tcgacgaata
    33181 tctggatgac acctttatgt tgttcagtag ttatggtatt aatatgcagg atcttcagaa
    33241 atggcggaag tcaggtaatc gactattccg ttgttttgtc aatgcgacga aagagaatcc
    33301 tgcgagttta tcttgttaga attattacaa ccatgggtag aagtatgtcc gaaaaacctt
    33361 taacgaaaac cgattattta atgcgtttac gtcgttgcca gacaattgac acgctggagc
    33421 gtgttatcga gaaaaataaa tacgaattat cagataatga actggcggta ttttactcag
    33481 ccgcagatca ccgcctcgcc gaattgacca tgaataaact gtacgacaag atcccttcct
    33541 cagtatggaa atttattcgc taataaataa ttcgctttcg gagctataac cggctgttta
    33601 ttaagaattt tatacttttt cgccatgaag acatacccta tgtgatcttt atcacacaga
    33661 tgtaatggga acgttctctt cactgacttt tcgtcttact gtgttgccgc attttcagca
    33721 accggaggtc agtaatgagc acagaaaaag aaaagatgat tgctggtgag ttgtatcgct
    33781 cggcagatga gacgttatct cgcgatcgcc tgcgcgctcg tcagcttatt caccgataca
    33841 atcattccct ggcggaagag cacacattac gccagcaaat tctcgctgat ctattcggtc
    33901 aggtgacaga ggcttatatt gagccaacgt ttcgctgtga ctatggctat aacatttttc
    33961 tcggtaataa ttttttcgcc aacttcgatt gcgtgatgct tgatgtctgc cctattcgca
    34021 tcggtgataa ctgtatgttg gcactaggcg ttcatatcta cacggcaaca catcccatcg
    34081 accctgtagc acgtaatagc ggtgctgaac tggggaaacc cgtcaccatc ggtaataacg
    34141 tctggattgg cggacgcgcg gtcattaacc ctggtgtgac cattggtgat aacgtcgtgg
    34201 tagcctcagg tgcagttgtc acaaaagatg tcccggacaa cgttgtcgtg ggcggtaatc
    34261 cagccagaat aattaaaaaa ttgtaatcgg tttttcgcaa ctgttatgca aaattgtggc
    34321 agatctgtta ctttccctct actattccca cgttaaaata gggtgttccc tggaaagttg
    34381 cagataccac gaaggcaaac gatgaccgaa atacaacgcc tgctgaccga aacgattgag
    34441 tctctgaata cccgcgaaaa acgcgacaac aaaccccgct ttagtatcag ttttatccgt
    34501 aaacatccgg ggctgtttat cggtatgtac gttgcttttt ttgccaccct ggcggtgatg
    34561 ttgcagtccg aaacgctttc aggctctgtc tggctactgg ttgtattatt tatcctgctt
    34621 aatggtttct tctttttcga tgtctaccca cgctaccgct atgaagatat cgacgtgttg
    34681 gatttccgcg tttgctataa cggcgaatgg tacaacacgc gctttgtacc tgccgcgctg
    34741 gttgaagcca tcttgaactc tccgcgtgtc gcggatgttc ataaggaaca actgcaaaaa
    34801 atgatcgtcc gtaaaggtga attgtctttt tacgatattt ttaccctcgc tcgcgccgaa
    34861 tcaacatctt aagttaggat tacataccag gcgtaaagct ctgcgcctgg tcaaatgaca
    34921 acgatcgttt ccacccatca cttcatgaaa taccagctct acctccttat ctcctgccag
    34981 cctttttcca caatcagata tactttccct acactgtgtt aataaggata tgctggtgag
    35041 aacacgacat ctggtcggcc ttatttcggg agtactgatt ctttcagtat tgctgcctgt
    35101 cggcttaagc atctggctgg cccatcagca ggtagaaaca tcgtttattg aagagctgga
    35161 tacctattcc tcccgcgtcg ctattcgggc caataaggtg gcgacacaag ggaaagatgc
    35221 gctgcaagag ctggaaagat ggcaaggcgc tgactgtagt gaagcccatc tcatggaaat
    35281 gcgtcgggta tcttacagtt atcgctatat tcaggaagtg gtttatatcg ataacaacgt
    35341 tccccagtgt tcgtctctgg agcatgaaag tccgcccgat accttccccg agccaggtaa
    35401 aatttcgaaa gatggttatc gtgtctggtt aacatcacat aacgatttag gcattatccg
    35461 ttacatggtc gccatgggaa cggcacatta tgtcgtcatg atcgaccccg cttcctttat
    35521 tgatgtcatt ccctatagct catggcaaat tgatgccgcc attattggca atgcccataa
    35581 cgttgtcata accagcagcg atgaaattgc tcagggaatt attaccaggc tacaaaaaac
    35641 acccggtgag catatcgaaa ataatggaat catttacgat atcctgccct tcccggagat
    35701 gaatatttca attatcacct gggcttcatc gaaaatgttg cagaaaggct ggcatcggca
    35761 agtctttatt tggttaccgc tcgggttggt gattggcctg ctggcagcga tgtttgtgct
    35821 gcgcattttg cgccgcatcc agtcaccgca tcatcggctg caggatgcta tcgaaaatcg
    35881 tgatatttgc gtgcactatc agccgattgt ctccttagcc aatggcaaaa ttgtcggtgc
    35941 tgaggcactg gcgcgctggc cgcagacaga cggtagttgg ttgtcaccag atagttttat
    36001 tccgctggca cagcaaacgg gcctttctga gccattgacg ctactgatta taagaagcgt
    36061 ctttgaagat atgggcgact ggctgcgtca gcatccacag cagcatattt cgatcaatct
    36121 tgaatccacc gtgctcacct cggaaaaaat cccgcaattg ctgcgtgaaa tgatcaatca
    36181 ctatcaggtt aatcccagac agatcgcgct tgaactcact gaacgcgagt ttgccgatcc
    36241 gaaaaccagc gccccgataa tttctcgcta ccgggaggcg ggccatgaaa tttatcttga
    36301 tgattttggt acggggtatt caagtttaag ttatttacag gatctggatg tcgacattct
    36361 gaagatcgat aaatctttcg ttgatgcgct ggaatataaa aatgtcacgc cgcatatcat
    36421 cgaaatggca aaaacactga aactgaaaat ggtagcggag ggaatcgaaa ccagtaaaca
    36481 agaagagtgg ctacgccagc atggcgtgca ctacggtcag ggctggctct acagcaaggc
    36541 attaccgaaa gaagatttct tacgctgggc cgagcaacat ttgtgaattt ttgggctgac
    36601 ggcacgccgc acagcccgtc acgctaccta ttcgtcgatg attgactcga atccgccata
    36661 gatcattcgc ttgccgtcaa acggcatgga ctcgccgaac tctttcatcc gtgggtccga
    36721 catcatcttt tgattagctg cgtcgcggac ctcttttgaa gggtattcaa tccagctaaa
    36781 gacaacctct tcattctctt ccgctttcac cgccatacga aaatcggtca ctttgccatc
    36841 cggtacatcg ctggcccagc attcgacaat acgaagcgcg ccaaactctt taaacaatgg
    36901 cgcggcctta gcggccattt cccgataggc atccttttta tcggcaggaa cggcaaccac
    36961 aaaaccatca acatacttca tcagacatac ctccgaagcg tactgcgcag ccagacaatt
    37021 cttgcgacgt cagttgtggt taaagtttag tcatgttggg attttttgac gaaacggtgg
    37081 gaggcagaaa tgggtggggg ccctgccagc tacatcccgg cacacgcgtc atctgccttg
    37141 gctgcttcct tccggacctg acctggtaaa cagagtagcg ttgcgggaga accaacagag
    37201 cccccattga gagcgttgag aaccaacgcg caggcgcatt atcactgctg ccccggctaa
    37261 ttgcaaccca ttgcgtgcca gatgctggtt tcttgcgcaa tgcggcttca ctcgggcgtt
    37321 tttccagatt atgctgaaaa attatctcag gaagaaaaga tggaaaaaga agattcattt
    37381 ccccaacgcg tctggcaaat cgtagccgct attcccgaag ggtatgtcac cacttacggt
    37441 gatgtggcga aactggcggg atcgccccgc gccgcgcgcc aggttggcgg tgtgttaaag
    37501 cgtctccccg aaggcagcac cttgccctgg caccgggtgg ttaatcgcca cggcaccatt
    37561 tcgctaaccg gaccggattt acagcgtcaa cgacaggcat tactggcaga aggtgtgatg
    37621 gtatcgggaa gcgggcaaat cgacttacag cgctatcgct ggaactacta acccctctcc
    37681 agatggaaag gggttagtaa cgcttagtat tgcgtagggg caggaactgc cgaagatgga
    37741 ttcacctggg tcggtgaagt cgatggtacg gtagttgttg cgccaccact ggcctggaca
    37801 ggcacggcgg tttgctgaac cggaaccaat gtcaggtcgg ctttggttcc cccctggttg
    37861 atcaccggct gaacggtatc ggtaataaat accagtttgt cattcacggt aatcgccgca
    37921 ctcaacagaa tacgcgcgtt cggctgaaca tctgccgggt taaatggcag aacaaagctg
    37981 aatggtgact gtttaccttc agtacgcacc gctttctgcg ccagcacttt tgacggtgca
    38041 tcggctaacg acgcgtcaga aagtgtcacg gtcagcacag catcaggcgg cagtgcgact
    38101 ttctgacgga tccagacggt accggagaca ttcggttgct ggatagcagg ttgttgtgtt
    38161 gctgaaatag acgtatttgc agccggggct ggcgtctgaa tatccgcgct tttatctgcg
    38221 caagccgcca acgcaatcgc aaccgctaaa ccactggcca tgtgcacgag tttcattcat
    38281 ttctccttat tatcaatgca ccagcgggct aactttcctc gccggaagag tggttaacaa
    38341 aatagtaacg tcaacaattg tggcacacat cacgcatttc tgcctgtaat tagcccgtaa
    38401 ttcagaccat tgcacccatc ggaccagtag caaatttgcg ttatactcaa ctcactttgg
    38461 cttgctgcgg cagctttgtt actggagagt tatatgagtc aggcgctaaa aaatttactg
    38521 acattgttaa atctggaaaa aattgaggaa ggactctttc gcggccagag tgaagattta
    38581 ggtttacgcc aggtatttgg cggccaggtc gtgggtcagg ccttgtatgc tgcaaaagag
    38641 accgtccctg aagagcggct ggtacattcg tttcacagct actttcttcg ccctggcgat
    38701 agtaagaagc cgattattta tgatgtcgaa acgctgcgtg acggtaacag cttcagcgcc
    38761 cgccgggttg ctgctattca aaacggcaaa ccgatttttt atatgacggc ctctttccag
    38821 gcaccagaag cgggtttcga acatcaaaaa acaatgccgt ccgcgccagc gcctgatggc
    38881 ctgccttcgg aaacgcaaat cgcccaatcg ctggcgcacc tgctgccgcc agtgcttaaa
    38941 gataaattca tctgcgatcg tccgctggaa gtccgtccgg tggagtttca taacccactg
    39001 aaaggtcacg tcgcagaacc acatcgtcag gtgtggatcc gcgcaaatgg tagcgtgccg
    39061 gatgacctgc gcgttcatca gtatctgctc ggttacgctt ctgatcttaa cttcctgccg
    39121 gtagctctac agccgcacgg tatcggtttt ctcgaaccgg ggattcagat tgccaccatt
    39181 gaccattcca tgtggttcca tcgcccgttt aatttgaatg aatggctgct gtatagcgtg
    39241 gagagcacct cggcgtccag cgcacgtggc tttgtgcgcg gtgagtttta tacccaggac
    39301 ggcgtactgg ttgcctcgac cgttcaggaa ggggtgatgc gtaatcacaa ttaaaaaaat
    39361 agccggaggt gaaaaccgtc cggctgtttt ttgcagtgct tgttatgcgt tataggcatt
    39421 ctcgccgtga ctgttgacgt ccagcccttc tcgctcttgt tcttccggca cacgcagacc
    39481 aaccgtcaga tccgccagtt tgtagccgat aaatgccacc acaccagacc acacaatcgt
    39541 aatggcaatg ctttccaact gtaccagcaa ctgatggccc atcgtcacac cttcagcgaa
    39601 gcccacgccg cccagcgagc tggcggcaaa aatcccagtc atgatacagc cgacaatgcc
    39661 acaaacgccg tgcacaccga agacatcgca gggatcatcc acccgcagca agcgtttgag
    39721 catggtaacg ccccacaagc ccgccagacc agctaccacg ccgataatca acgcgccgcc
    39781 aacaccaatg tagccacacg ctggcgtcac gccgaccaga ccggcaatcg cgccagaaca
    39841 cgctcccagc agtgaaggct taccacgcag cgcccattca ccgaagatcc agccaagaat
    39901 tgccgccgcc gttgcgacca cagtattcac aaatgccagt gccgcgattt cattcgccgt
    39961 gcccgctgac ccggcgttaa agccaaacca accgatatag agaatggcag tcccggtgaa
    40021 gaccatcggc aggttgtgcg gtttaaacgc ctctttaccg aagcccacgc gtttgcctat
    40081 cagatacgcg cccaccagac cggcgattgc ggcgttaata tgcaccaccg tgccacccgc
    40141 gaaatccagc gcaccgtgag aagccagcaa accaccgccc cacaccatat gcgcaatagg
    40201 aatgtaagag agcgtcagcc ataccaccac gaaaatcaac acagctgaga agcggattcg
    40261 ttccgccagc gccccaacta tcaagcctac ggtaatgcag gcaaacgatc cctgaaacgc
    40321 tacgtggata tactgataaa tgctgcccat caccgccgtc agttcgatgt ttttcagcat
    40381 caaccagtta atgttgccga agaagttgtt gccctcacca aacgccagcg agtaaccgta
    40441 aaccacccag agaatacaga ccagtgcaaa cgtcaccgtc acctgcgtca gcatcgacag
    40501 cacgtttttg ccgcgaatca acccaccgta aaacagggca atccccggaa tagtcataaa
    40561 cagcaccagt gcagtacaaa tcatcataaa cgcattgtcg gctttatcgg ccaccgcagg
    40621 tgcagccatt accagtcccg gcagcatcgc cagtgaagca agcccagttt ttatcgtcgc
    40681 tatcttcatt tttcgttcct gttgctgtgt gccagagatt acagcgccgc ttcgtcggct
    40741 tcgccggtac gaatacgaat gacgcgttgc aattcagcga cgaagatttt gccgtcgcca
    40801 atttttccgg tgtaagccgc cttactgacg atatcgatca cttcatcgag ttggtcatca
    40861 gcaatcgcca catcaatttt tacttttggc aggaagttga cgctgtattc cgccccccgg
    40921 tacagctcgg catgcccttt ctgacgcccg aaacctttca cttcggtgac ggtcaggccc
    40981 tgaataccaa tggaagataa cgcttcacga acgtcttcca gcttgaatgg tttgattatc
    41041 acggtcacca gcttcataga tcccctccgg tcagaattcg gtaatggttc tgctacacga
    41101 agaaggtatt gcaagcggtg tgccagaaat gaaaaacgag aaagagagca ggaagttaag
    41161 aagattgaaa atgaaaacgc actatgacag tgcacagtgc gttacatttt tgcaccaacg
    41221 atgagatatt gcgctattca ggcgctcaat gattcttctt cacgcacgct ggctgcaagc
    41281 tcttcgcccg caagttgcag ttgatacatc tgccagtagc gtccctgggc cgccagcagt
    41341 tgctggtgag tgccctgctc cacggcttgc ccacgatgaa gcaccagaat agtgtcggca
    41401 tcaacaatag tcgataagcg gtgagcaatc actaccagcg tggtatgttc acgcaccgcc
    41461 gccagagcat gttgaatcgc ctgttcagta ccggagtcaa tgctggcggt tgcctcatca
    41521 aggatcagga tttgcggcgt ctcgaccagc acgcgcgcca gtgccagcag ttgcttttgc
    41581 ccgacagaga gattattccc ctgctcgccc agcggcgtat aaatcccgtc gctcatactg
    41641 cgtgccagct ccgccagttg cacggtttcc agcgcctgcc agacgcgttc ttcggagata
    41701 tcccgcccca gcgtcacgtt ggcgaggaag gtatccgcca gcaccaccgg atcttgctgc
    41761 accattgcca cgccctggcg cagcgcgctg tgacttagcg aacttaatgg acgaccatca
    41821 aggcgaatct caccttccgt tagcgggtaa tagcccatca ataaactggc gagggtactt
    41881 ttaccactgc cggtatgccc gaccagcgcc acaaagttac gcgaaggcac agagagatta
    41941 atgttcttta gcaccagatt gtcatcgcga taagcaaatg acacgttatc gacttcgatg
    42001 gtgccactct gtaacgggcg atcatcattg ccatattgct ggcgcggtcc gtccatcagc
    42061 tcaaacacgc gctcaccagc aacaacagcc tgttgcagca tcgcctgctg cgtggtcagt
    42121 tcgattaagg gttcgttaag tcgcccaaga tagctgataa acgcatacaa cacgcccact
    42181 tcaatggtgc cgctggcgga gaagccaaac agcatcaaca agccacagag aatgagcgat
    42241 gaaaacagac tcagcagcgg acgcagcaga aaaccgtcga ggcgcagggt ttgcatcctc
    42301 gccatatagt gtgaacgact ggcctcaccc atacgttcgc caaatcgcgc ctgctgacga
    42361 aactgctgga taacgctcat gccattgatg atttcgttaa agccgtcgtt gatatccgcc
    42421 agataggcgc gcacacgacg gacaattggc gtgctgtaac gctggtatat caccattacc
    42481 accagcacca ccgggaaaat cattatcgcc accagcgcca ttcgccagtc gaggctgaac
    42541 atcgccacca gcatcgcgcc caccagtgcg gcactgcgca ggacagttgc cactacggta
    42601 acgtagagat cgcggatcac ttcagtgtca ttagtgacgc gggaaatcac ctgcccgacg
    42661 ggttgggtat caaactcgct taatggctgg cgtaacgcag catccatcac gtcggtacgc
    42721 aactgttgca ctacgcctac tgccgcccga ttaaacagca gcgactgcgc gtaatgtagc
    42781 ccggcggcaa acagttgcag cccaacatac gccgcagcca gccctgcaac cactttcaac
    42841 ggcaggttat ttttcgctac catattgtcg ataaaatagc tgataagcag cggcccactg
    42901 acttctgccg ccgccgcaac ccacatcatc aggaccgcaa tccccagcgg tttacgccac
    42961 ggtgaaccgt acgctaacag gcgcttgaga gtcggccaca gttggctaaa actacgcatc
    43021 gacggcctcc tcgcgatttt ccggagcgtc gtcgagcgcc gcctccagtt gttgatagcg
    43081 atacatatcg cgataccagc cgctttgttg tgccagcaca tcatgattgc cacgctgggc
    43141 gatatgtccg tgctgcatca caataatttc actggcttcc gtcagtgcag aaaggcgatg
    43201 ggcactgatg attaccgttc ttccctgccc ccactgacgc aggttatgca ggatctggtg
    43261 ctcagtgcgt ccgtccaccg ccgaaagcgc atcatcaagg atgaggattt ccgcgttgac
    43321 taataacgca cgagcaatgg agatacgctg tttttgcccg ccggaaagca tcacaccgcg
    43381 ctcgcccacc tctgtatcgt aaccttgcgg tagacgcaaa atatcgtcat gtacgctggc
    43441 taaccgcgcg acatgctcaa tctcttgctg ggtggcattc gggcaaccca gcgcgatgtt
    43501 attcgccacg gtgtcagaaa aaaggaatgg cgtctggcta actaccgcca ggcggctacg
    43561 ccagctatcg agttgtaact tcgtcagagg aatatcatga aagcgaatat ccccctcgct
    43621 gacgtcgaaa tgacgctgaa tgagcgacaa cagggtactt ttgccggaac cagtcggccc
    43681 gcagataccc agcatctgac cgggtttcag ggcgaaattg acgttttcca gcgcaggatg
    43741 gtcagtctgc ggatacgtga actggtgaat atttacatcc agttcgccac gcccttccgg
    43801 cacgggttcg ctaccatcgt tcaccaccgg cgcttccgcc agcatcgcgc gaatacggct
    43861 gtacgcagca ctaccacgtt ccacaatgtt aaacatccat gccagcgcca gcattggcca
    43921 aatcatcaga cctaaataca tcataaaact ggtgagctgg cccagcgtta aactgccctg
    43981 caccaccatc caactaccgc cgccaatcgc cagcaagttc gccataccaa tcgcgatata
    44041 gatggtcggg tcgaaacgag catcaatacg cgccacccgc atgttttttt tgccggtatc
    44101 ttcggcatcc gcggcaaata acgccgactg gcgatcttcc agaccaaagg ctttgatcat
    44161 gcggatactg gtgaggcttt cctgggtgcg gtcattaaga ctggaaaacg ccgcctgtgc
    44221 cagcttaaag cgttcatgca aagcatcgcc gttgcgctta atcatgatcg ccatcactgg
    44281 catcggtaac agggcaaata aggtcaactg ccagctaatt tgcgtagaca tcataatcaa
    44341 cacagcgcag cccattacca gtgaatccac cagcgtcagc accccttctc cggcggcaaa
    44401 cacgacgcga tcgacgtcat tagtcgcacg agccatgagg tcacccgtgc gatgacgcag
    44461 gtaaaactca ggatgctgcc ggcttagctg acggtaataa tcttcacgca gttcaacagc
    44521 cagttgataa gacgcaccaa acagcaatac gcgccagacg taacgcagga gataaaccac
    44581 aacggcaatc agcaccatgg tggcgatcca catcaggatc tgcccggtag taaagtgttg
    44641 ttctgtcacg ccatcgacaa caataccaac cacttttggt ggaaccagtt gcagcatcgc
    44701 gataatgaca agcaaggcga cagccccgag atagcgacgc cattcccgac ggaaatacca
    44761 gcttaattga gcaaataatc gcacgcgttg tgtcctgacc tgattctgga tatttattcg
    44821 atgggtaaag aagtggtgta tttaatctgt tccatcgcga agctggaagt gacgtccgac
    44881 agccccggca cgctgtttac cagacgctta taaaactcgt cgtagcgttt catgtcggca
    44941 acctggacgc gcatcagata atcgtattca ccagccatgc gccagaaccc cagcacttct
    45001 ggcatttcgg taaccaccgt gacaaagcgg caataccatt cgctgctgtg atgttgcgtt
    45061 tttatcagca caaaggcggt caggccgagg cctatttttt ccggatccag cagggcgact
    45121 ttgccgataa ggataccgtc gtcctccagc cgtttcaggc gcttccagca gggggtggtt
    45181 gtcagattaa cggcttcagc cagtgcctgc aaagagaggg tgcaatcctg ctgtagtaag
    45241 gccagcagct tacggtcaat tttatctaac atagcccttc cacggagaat ttttttctct
    45301 tttaattcta ttttaaaggt gaaatcgcga caatttattc tgtgcggttt aaacaccacg
    45361 gcacaaaatg acaaattcaa cgcaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
    45421 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
    45481 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
    45541 cggcatcgca ttgcccataa taaatccgct accgacgctg cctaacattt cgcgatcgtt
    45601 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
    45661 cgtcagtgca gcgcctttat tgcagcccac cggcagcact tcaaggcaat ccgtggcgga
    45721 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtgtagc tggatctgca aacgtgtaag
    45781 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
    45841 atcgattatt tgataacgaa aaccgctata gacaaatgcc tgcaacaacg cagggatctc
    45901 tttcccggta aaccaaccgt cgtcattgaa aatatgcatg ctggctcggg tatcccattg
    45961 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
    46021 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tacgcatcca gcgatatcgc
    46081 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
    46141 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
    46201 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
    46261 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
    46321 ataatattgt tccgggtttt tatgcgactg ctcaaccgcc ttaaccagta tcaacgtctg
    46381 tggcaacctt ccgccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
    46441 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaagc gggatggctg
    46501 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgcca
    46561 gaatcgctac gcaatgcgat gatggaaaag gcactggaaa ccggaaagca gcaagatgtg
    46621 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
    46681 ggacaatggc aaaacgatac acccacgttg cgtattccct actatcgccc gctcgaaccg
    46741 ctacaaccgg gctttttgcc cggccgtgcc gagcagcatc tcgccggaca gatattttcc
    46801 ggcctgaccc gcttcgataa taatacccag cgcccgattg gcgatttagc gcatcactgg
    46861 gaaacctctg ctgacgggtt acgctgggac ttttatcttc gttcaaccct acactggcat
    46921 aacggcgatg cagtaaaagc ctcacactta caccagcgat tattgatgct gttacaactg
    46981 ccagcactgg atcaattatt tattagcgtg aagcgtattg aggtcaccca tccgcagtgt
    47041 ctgaccttct ttttacatcg ccccgattac tggcttgcgc accggctggc gagctattgc
    47101 agccatctgg cgcatccgca attcccactg attggcacgg gtccttttcg cttaacacaa
    47161 ttcacagctg aactggtgcg cctggaaagc catgattatt accatttacg tcatccgctg
    47221 cttaaagcgg ttgagtactg gataactccg ccgcttttcg aaaaagattt gggaaccagt
    47281 tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg agctgcaacg ggtcagccag
    47341 gtcagtagtg gcatcagttt aggtttttgc tatttaacgt tgcgcaaaag tccccgactc
    47401 tccctctggc aggcgcgaaa agtgatctcc attattcatc aatccggttt attacaaacg
    47461 ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat tactgccagg ctggactatt
    47521 ccgcattggc aagtaccgga tgaagtcaaa ctaccgaaaa ccttgacgct ggtttatcac
    47581 ctcccgatag aacttcatac catggcagaa cgcctacagg cgacactggc agcggaaggc
    47641 tgtgaactca caattatttt tcataacgca aaaaactggg acgacacgac cctactggca
    47701 cacgcagacc tcatgatggg cgacagatta attggcgaag caccggaata tactctggag
    47761 caatggctgc gctgcgatcc gctgtggcca catgttttcg acgctccagc atacgcacat
    47821 ctacaatcga cactggacgc ggtgcaagta atgcctgatg aggaaaaccg atttaatgcc
    47881 ctgaaagcgg tttttagcca gttaatgaca gatgcgacgc tgacgccgct gttcaactat
    47941 cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc gactgacacc gcgcggctgg
    48001 tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat gaatagctgg tccgggttaa
    48061 tcgcaggcgt taccataacg cttttattgt tcaagcagga ttatctatga aacgtgctgt
    48121 cgttgtgttc agtggaggcc aggattccac cacctgtctg gtgcaggcat tacaacaata
    48181 tgatgaagtc cattgcgtga cgttcgatta cggtcagcgc catcgcgcag aaatcgacgt
    48241 ggcacgcgaa ctggcgctga aactgggggc acgcgcgcat aaggttcttg atgtaacgct
    48301 gctcaacgag ctggcggtca gcagcctgac gcgtgacagc attccggtgc ctgattatga
    48361 acctgaagcc gatggcatcc cgaatacgtt tgtcccaggg cgtaatattt tgttcctgac
    48421 gctggcggca atatatgcgt atcaggtaaa agcagaagcc gtaattaccg gcgtctgcga
    48481 aacggatttc tccggctacc cggattgccg cgatgagttt gtgaaagtac taaaccatgc
    48541 cgtcagtttg ggcatggcga aagatattcg ttttgaaacg ccgctgatgt ggattgataa
    48601 agcggaaacc tgggcgctgg cagattatta cggcaaactg gatttagtcc gtaacgaaac
    48661 gttgacctgc tataacggca ttaaaggcga cggttgcggt cattgtgcgg catgtaattt
    48721 acgcgccaac ggtttgaatc attatctggc cgataaaccg acggtgatgg cagcgatgaa
    48781 gcagaaaacc gggttgaagt aattattccg ggtgtcgccg gatgcggctt gagcatccgg
    48841 caccacaaaa cgtttactta accatctgct ccagcttttc gcgcaattcc ccttccagag
    48901 ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt aataagcgca tccgctacca
    48961 cctgcccttc cggctccagt gtaatgacct ggcttaagat gccgctttta ccgtttaatt
    49021 gctgtaactg gctcgtaatg gttaacaagt cgcttaatac cgccggacga cgatagttaa
    49081 tattgatatt caccaccacg aaagcgatgt tatgggccgt catccagtga aaactgtcgc
    49141 tattttccaa cccatcccag cgggcttctt cgagaaattc aaggtagcgg gcgttgttta
    49201 cgtgctggta aacgtcgaga tgatatccac gaactttgat ttgtgtttgc atagcgcaat
    49261 aaccttacgt tgtttttata aaaactgtta taagtacaga ggtcataact ggtatgacct
    49321 cttcagtctg gcaaaaaatt gccactatgc aaattaatta cagggttaat accgccagat
    49381 tacgttccac cagcgaattg cccatccccg gcacctgctt tagatcctcc accgttttaa
    49441 acggaccgta ctcttcgcga taactgacaa tcgcttgcgc tttcttcagg ccaacgccat
    49501 tcatcgcgcg ggctagctct tccgcgctgg cattattaat gctgacccgg gagccttctt
    49561 cgtcactggc tttcgccggt actgctgctt tattttgtgc tgcaggagct tccgctttgg
    49621 tttctaccgc cgtcggtttc gccacagaag ctgccgccag cgcgctatga gacattccgg
    49681 cacaggccag ggacagggta atgagcagtg ctttaattcc gtgtttcata ctgttttctc
    49741 cttgtttgtt aacagtggaa tcaccttaac ggcagtgaaa acagtaaaca aatggcaatg
    49801 ttcagaaatg gaaaaggccg cgaaagcgac cttttcttgt tacagctgca aagcattttt
    49861 ttgcgaggcg gcttcaggat tattgctgtt ccaacgcatc gccaattttg attttcgcct
    49921 ctttacgcag gttactcatc agagcttcaa agacgatttg tgcgttgttc tgggtgatac
    49981 cctgcaccat cgcttttttc tgatcttccg gcattgaacc ttgtttcact tcatccagcg
    50041 ccagcagaac cacattacct tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg
    50101 ctggcagtgg cagtgcaaac gccgcctggc taatcgggtc acgaccggaa cggcttaagg
    50161 ttttcggctc gccaaatttc agaccggcag cctgcatagc ttccgcacct ttgccggctt
    50221 tcaaatcaac cagcagtttc tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct
    50281 gaaccagtgc cttaacttgt tcctgaacat ctgccaacgg tttcaccgct tccggtttgt
    50341 gctcgctgat gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg tcagagttga
    50401 tgcccggcgc gccgttttca cctaccagac cgccgttaaa gatagcgtca gcaaccggct
    50461 tgaagttcaa ctcttccggc aggttctctt tgctgaacca acccgtctga gtggctttaa
    50521 cgccggcagc ttgctctgca ccggccagag actcggtgtc gttgcttgcc gcatcgctca
    50581 ctttctgctg cagcgcgtag tacgcatcga gggctttttc gtgtttcact ttcgccgcga
    50641 catcgtcacg tacttcgtct aacgatttca ctttcgctgg ctgaatgtcg tccagacgta
    50701 caatcaggaa accgaccgaa gatttgatga caccagacag ttggcctttt tctttcagac
    50761 cagcattttt cagttcatcc gggatagtgg catcttctaa ccaacccata tcgccgccgt
    50821 tacgagcaga gataatatcg gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt
    50881 tattcagctc atcaagtacc gctttcgctt catcttcagt tttggtctgg atgatgctgt
    50941 aacgggtacg ctgcggctgg gtgaattgat cctgatgctg atcgtagtag ctctggagat
    51001 ccgcatcgct aaccggttgc tgcatcgttg cggcatccag cttgatgtaa ctcacgcgga
    51061 attgttccgg cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca atttcctgtt
    51121 cggtcacagg ctgcttcgcc gccagcgcgt taacatcgat agtcgcttca cgcaccacgc
    51181 gttgttgagc gaccagtgcc gccagctcgt cggtttcacc tttcagcata aaatcggtac
    51241 cagcaacgcc gttaatcagc tgttgggtag tgagctggtt acgcagcgcc tgggcgtact
    51301 gatcggcggt catccccatc tggttgagga taccgttata gcggctgtta tcaaatttgc
    51361 catcaacctg gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca tcgctgatac
    51421 ccagtttcag ctcacgtgcg tactgatcca gcagcgcctc gtcgatcaga cgattcagca
    51481 cctgttgacg cagggttttc atatagcctt cgttcgctgc cagctcggag tattgatcgc
    51541 ccagctgttg ctgcatgcga ttacgctcgc tgttgaaggc attctcgaat tgcccacggc
    51601 tgatttcctg gtcattcact tttgcggcgt agttattgcc tccgccaatc aggtaaccac
    51661 tcacgccggt caatatgaac gacacgataa tgataccgaa aataatcttg agcacgagac
    51721 tgtttgcagc cgtgcgtaag ctgtccatca tggtgtaaca acactccgct gtagatgact
    51781 gtgtacctca cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca
    51841 attgtcagcc cacaagacgc agaaagtcac cgaatacaaa taaaaaaggc acatcagtag
    51901 atgcgccctt gaacttcgtc acatccccac tggggacaac gcttagttta ccgcgtcttt
    51961 cagtgcttta cctgcacgga agctcggtac tttagcggca gcgatggtga tctctttacc
    52021 ggtctgcggg ttgcggccag tacgggcagc acgctcttta acggcaaaag taccaaaacc
    52081 taccagtgct acatcatccc cttctttcag agattcagtt acggaagcaa taatagcatc
    52141 taacgcacgg ccagccgcag ctttagagat atcagcccct gcagcaatct tgtcgatcaa
    52201 ttgagattta ttcactcttc tcttcctctt tataatttat atcgcacttg aatccttcaa
    52261 ggtacgaacg cgcagcagtt atatcaggcc agccatcccc ttacaacacc ccttaataag
    52321 ggcaagcccg atccgccatc taacttagcg agacaaaaaa aggctggcaa gcccgaatta
    52381 gcctgccagc cctgttttta ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa
    52441 cctgcatgcc agacggttca ttttgcagcg ccagagtcag aacttcctca atgcgcttca
    52501 caggatgaat gtccagatcg gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat
    52561 tttcgaacgg aattaacact gttttaatcc cgccgcgatg cgctgccagt agtttttctt
    52621 tcaaaccacc gatcggcagt acctgaccac gcagagtgat ctcaccggtc attgccacat
    52681 cggcacgaac cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg
    52741 cactcggacc atctttcggc gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt
    52801 cgtaaaaatc agggttgatc cccagttttt ccgcacgcgc gcgaaccacc gttaacgcag
    52861 cctgaataga ctcctgcatc acttcgccga gcgaaccggt ataggtcagt ttgcctttac
    52921 ccggaacaca tgcggtttca atggtcagca agtcaccgcc cacttccgtc cacgccagac
    52981 cggttacctg accgacacgg ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac
    53041 caaggtagtc atgcaggttg tcgccgttaa tttcgatatg ttttaatgac ttatcgagca
    53101 gtaactgctt aaccgcttta cggcacagct tggagatttc acgctccaga ccacgcacgc
    53161 ccgcctcacg ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg acggtcagct
    53221 cacctttttt cagtgcatta cgttcaatct gcttcggcag caggtgacgt ttggcgatgt
    53281 tcagtttttc atcttcggta taaccggaga ggcgaatcac ttccatacga tccagcagtg
    53341 gtgccggaat gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat
    53401 ccacttccag gtagtgatcg ctgaaggcca cgttctgctc tggatccagc acttcaagca
    53461 gtgcggaagc cggatcgcca cgcatgtcag aagacatttt gtcgatctca tcgagcagga
    53521 acagcgggtt tttaacgccc actttcgcca ttttctggat caatttaccc ggcatagaac
    53581 cgatgtaagt acggcggtga ccacggattt ccgcttcatc acgcacgccg cccagcgcca
    53641 tacggacata tttacgcccg gtggctttgg caatggactg acccagggag gttttaccta
    53701 cccccggcgg ccctaccagg caaaggatcg gtcccttgat tttgttgaca cggctttgaa
    53761 ccgcaagata ctcaaggatg cgatctttca cgcgctccag accataatgg tcggtattaa
    53821 ggatttcctg cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc cacggtacct
    53881 gtaccatcca gtcgatataa ccacgcacta cggtcgcttc tgccgacatc ggagacatca
    53941 ttttcagctt ctgcaactct gcttccgctt tctcttttgc ctctttcggc atcttcgccg
    54001 cgtcgatttt gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatc tcaccgagtt
    54061 ctttctgaat agctttcatt tgctcgttca gatagtactc acgctgggat ttctccatct
    54121 gctttttaac gcggttgcga atgcgtttct caacctgcag cagatcgatt tccgattcca
    54181 tcattgccat cagatattcc agacgttcgt taacgtcgga catctccaga acggactgtt
    54241 tgtcagccag tttcagcggc atatgtgcag caatggtatc cgccagacgc gccggatcgt
    54301 cgatgctatt caacgacgtc agcacttctg gtgggatttt tttgttcagc ttgatgtagc
    54361 cttcgaactg gctgattgca gtacgcacca gcacttcctg ttctcgctca tcaatggtcg
    54421 gcgactccag atactccgcc ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa
    54481 tacgcgcgcg ctgtaacccc tcgaccagca ctttgacggt gccgtcaggc agtttcagca
    54541 tctgcaatat agaggccacg gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg
    54601 ttgaagcttc tttctgcgcg accagcataa tttttttatc atggtccatc gccgcttcca
    54661 gacaacggat agatttttcc cgcccgacaa ataaggggat gaccatgtgc ggataaacca
    54721 ccacatcgcg cagcggcaat acggggattt caatgcgttc agaacgctca ggattcatag
    54781 agctctctct tagtttaatt tccgccaggt aatcagatga cacgactgtg cttcacgcca
    54841 tctattaaca tgtacgtcag tatatgggga tgtttccccc acattcaacg ccgagaatag
    54901 aggaaaaatt aaaggggaga taaaatcccc cctttttggt taactaattg tatgggaatg
    54961 gttaattatt caccagatgc ctgttgcgct tccggcttgc cataaatcag caacggtttg
    55021 ctttgaccat caattaccga ctcgtcgata accacttttt cgacatcttc catggacggc
    55081 agatcgtaca tggtatcgag cagtgcggct tctacgatgg aacgcaggcc acgggcaccg
    55141 gttttacgcg ccatcgcttt cttagcgata gcatccagcg cctcgtcacg gaattccaga
    55201 tccacgcctt ccagattaaa cagcgcctga tactgcttgg tcagggcgtt tttcggctct
    55261 ttgaggatct gaatcagagc ttcttcgctc agttcattca acgttgcgac aaccggcaga
    55321 cgaccaataa actcagggat aagaccaaac ttgatcagat cttccggttc aacctgcgcc
    55381 agcagctcgc cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg
    55441 ccggagccgg tttctacacg atgggaaatc actttatcca gaccggcaaa cgcaccgcca
    55501 caaataaaca ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgt
    55561 ccaccttgcg gtggaacagc agctaccgta ccttcgatca gtttcaacag tgcctgctgt
    55621 acgccttcac cggaaacgtc acgggtaata gacgggttgt ctgacttacg agaaatcttg
    55681 tcgatttcat cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt
    55741 tgcaacagct tctgaatgat gttttcaacg tcttcgccca cataaccggc ttcggtcagc
    55801 gtggttgcgt cggccatggt gaacgggacg tccagcaggc gcgccagcgt ttcagccagc
    55861 agcgttttac cggaaccggt cggaccgatc agcagaatgt tacttttgcc caactcgacg
    55921 ccattgctgg tatcgccgtt gcgcagacgt ttgtagtggt tgtataccgc gaccgccagc
    55981 acttttttcg cctgttcctg gccgataacg taatcgtcca ggtggttacg aatttcatgc
    56041 ggcgtcggta gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg
    56101 atgtcgttac ataaatcaac acattcgtcg cagatataca cggatggacc ggcaatcagc
    56161 ttgcgcactt catgctggct tttgccgcaa aaagagcaat acagcaattt gcctgagcca
    56221 tctttgcgtt tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac
    56281 gacgcaaatg ccattctcag gcgcaagccg cttacagcgt tgtgccgccc tggataagta
    56341 tagcggcaca gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa tcgaccagac
    56401 cgtattccac cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca cgttcaatct
    56461 gttctaatga ttgacccgta tgaagcgcca taagttcatt catacgccct ttaactttca
    56521 gaatttcacg ggcatgaatt tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt
    56581 ggtgaatcat cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc cctgcggtca
    56641 gcaagaaagc gcccatcgag gccgcctggc ccatacagat ggtgctgaca tcaggcttga
    56701 taaactgcat ggtgtcatag atagacatcc cggcagtgat caccccgcct ggggagttaa
    56761 tgtacagata gatatctttt tctgggtttt ctgcttccag gaacagcatc tgagccacaa
    56821 tcaggttagc catatggtct tcaacctggc cagtcagaaa aatgacgcgt tccttaagta
    56881 gacgagaata gatatcaaaa gagcgctcac cacgtgaggt ctgttcaatg accatcggca
    56941 ccagcgccat atggggtgca aagttatctc gttcgccgct gtatgacatt tccgtctcct
    57001 ggataaaatt gaaaaaacct gctgtaccga ttgtaacctt atggacggat tatcagctag
    57061 tccctgtgtt atgggtacgg catcaccata tttcaagcat aacaatcttt tgttgttacg
    57121 ctaacaccga aagaggcttt tcgcacggtt ccatgcaaaa ttcatgacaa aaaaagccca
    57181 ccacctggcg gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt aaattacgcc
    57241 tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc tttcgccagt
    57301 acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat cagttctttg
    57361 tttttgctgt agaactcgat aacttctttc ggatcttcgt acgctgaagc catctcttcg
    57421 atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat aacttcgccc
    57481 agcagcaggc caacaactac gcggcgttta gcctgttctt cgaacagttc gcgcggcagt
    57541 tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg cagaacgtcg
    57601 atttcgctgt caataagcgc agccggtaca tcgatgtcgt tagcttttac cagaccttcg
    57661 atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc catgttttta
    57721 cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg tttgatgaat
    57781 tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat agcgaatttc
    57841 gctgctttac ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac gtcgatggtg
    57901 aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat catacgaccc
    57961 tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc gccgtctaca
    58021 gaaccggtga agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc tttttctttc
    58081 caggtcgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc gtcggtcact
    58141 tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac ttccggataa
    58201 acttcaaact ctacagagta ggtgaagtct tcacccagct tgtattcgcc cggaacataa
    58261 gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa gttacggctc
    58321 atcaggtcac ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc aacgatattc
    58381 attggcactt tgcctttgcg gaagccgtca atacgtactt ttttcgcaac gttgaccagc
    58441 tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac acggcggcca
    58501 aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat cacagtgctc
    58561 ggtcaactct taccccgcaa gcacaggttg ttggcttcgc ggaaccggga tgttcttata
    58621 atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg acgcggcatt
    58681 atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg tattttttca
    58741 caattcccgg gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc agacaaaaca
    58801 aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa agcgccgaca
    58861 gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag aggcaaagac
    58921 ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca gcgcatgaac
    58981 ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat tcagaaaacg
    59041 ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag agccggctgg
    59101 gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga acgccgccct
    59161 taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac aacgctgaga
    59221 tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc ttaacaacaa
    59281 atatttaggt ttcgtcctgg tttttccgtc aagcgtatga agtttactga tttaaagatt
    59341 gaaacacagg cctcgcatca gcgtttcttt taccaggggc gattatttcg tcaacatgat
    59401 gaatttacat gccttaccta cttcccctcg ccgttggcaa tgttatgatg gcgggaattt
    59461 ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa tcctcttccc
    59521 gttagttgct ctgtttatgc ttgcaggatg cgcaagaccg ccaacaacta ttgaagtttc
    59581 cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg taagcattaa
    59641 tggtgccgat cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata atcaaatcgt
    59701 taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg aaaaacagat
    59761 gaccgcgcgt ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa tcatcgttag
    59821 ccagctgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta ccaaagcgga
    59881 tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact atcgtgccag
    59941 ctacaacgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg cggttaacag
    60001 tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcattcacg aattcatcaa
    60061 gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg tagacgccca
    60121 tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg ctgatcctgg
    60181 gttttgcttc cgggctaccg ctcgccctga catccggcac cttacaggcc tggatgacgg
    60241 tcgagaatat cgatctcaaa accattggtt tcttctctct ggtaggccag gcttacgttt
    60301 ttaaattcct ctggtcaccg ctgatggacc gctacacgcc cccatttttt gggcggcggc
    60361 gcggttggct gctcgccacg caaatcctgt tattagtcgc cattgcggcg atgggttttc
    60421 tcgaaccagg cacccaactc cgctggatgg cggcgctggc ggtggtgatc gctttttgct
    60481 ctgcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg gcagaagaac
    60541 gtggtgcggg cgcggcaatc agcgttctgg gttaccgttt agggatgctg gtttccggcg
    60601 gcctagccct gtggctggca gataaatggc tgggctggca gggcatgtac tggttgatgg
    60661 cggcgctgtt gatcccctgt attatcgcga cgttgcttgc accagaacca accgacacca
    60721 ttcctgtgcc caaaacgctg gaacaagcgg ttgttgcacc tctgcgagat ttctttggtc
    60781 gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc gacgcattcg
    60841 ccatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg ggtgaagtag
    60901 gcgtggtaaa caaaacgctt ggcttactag cgaccattgt tggcgcattg tacggtggga
    60961 ttttgatgca gcgcctgtca ctgttccggg cactgctgat tttcggcatt ttacaaggtg
    61021 cgtctaacgc tggttactgg ctgctgtcga ttactgataa gcatctctac agcatgggcg
    61081 cagccgtctt tttcgaaaac ctctgtggcg ggatgggcac atcagccttt gtcgcgctgt
    61141 taatgacgct atgtaataag tcattttccg ctactcaatt tgccctgctc tcagcgcttt
    61201 ctgctgtagg gcgagtttat gtcggccccg tggcgggttg gtttgttgaa gcacacggct
    61261 ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc agggcttatt ttgctgctgg
    61321 tttgccgcca gacgcttgaa tatacacgag taaatgacaa ctttatctcc cgtaccgcat
    61381 atccggcagg ttatgccttt gccatgtgga cactggcggc gggcgtcagc ctgttggccg
    61441 tgtggttact gctattgacg atggacgcgc tggatttgac gcacttctct ttcctgcctg
    61501 ctctgctgga agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt ggtttgctgg
    61561 attatctggc gctacgaaaa acgcatctga cgtaatctgt aaatattatt tagctgtagt
    61621 aatcactcgc cgtaattatt acggctaaat aaacatcagc atcgcttatt aatatttgtt
    61681 cttttgtgcg gcttagcgtt tggaaattat tggcgccatt tataaattct atttttctta
    61741 cacgattcag ctaatgagtc tttattttct caccaccaag ttgtcactcc aatgataatt
    61801 atttgttaaa taattgtttt atttcgcatt gattatacca attgcccgtc cagacttctt
    61861 ctgcactccc cttttgttat aacgcccttt tgcaacagct tcttaaaatc aacctgatat
    61921 gttttgcaac atatgtgacc tgacagccaa atccaagtaa caggaattta atcatgttta
    61981 cagtaatgta accttcccgt aaaatgccca cacattttaa acgccaccag atcccgtgga
    62041 attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg ttgtcattat
    62101 ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc aaaggacaga
    62161 ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg attgtcgtta
    62221 ttcccgcaat cttgatggct gttggtttcg cctggaagta ccgtgcgagc aataaagatg
    62281 ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc tggacggtac
    62341 ctatcttaat catcatcttc cttgcggtac tgacctggaa aaccactcac gctcttgagc
    62401 ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt tccatggact
    62461 ggaaatggtt cttcatctac ccggaacagg gctttgctac cgtgaatgaa atcgctttcc
    62521 cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac tccttcttca
    62581 ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc ctgcatctga
    62641 tcgccaacga acccggcact tatgacggta tctccgccag ctacagcggc ccgggcttct
    62701 caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgcattcgac cagtgggtcg
    62761 caaaagcgaa acagtcgccg aactccatgt ctgacatggc agcgttcgaa aaactggccg
    62821 cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccaaac ttgtttgccg
    62881 atgtaattaa caagtttatg gctcacggta agagcatgga catgacccag ccagaaggtg
    62941 agcacagcgc acacgaaggt atggaaggca tggacatgag ccacgcggaa tccgcccatt
    63001 aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca gtcccgttcc
    63061 atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg gcgctcgttg
    63121 gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg acctccgtcg
    63181 accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg ctgcgtggtt
    63241 ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggc gaagcgggct
    63301 tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt atgatcttct
    63361 tcgtggcgat gcctttcgtt atcggtctga tgaacttggt ggttccgctg cagatcggcg
    63421 cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttacc gttgttggtg
    63481 tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc tggctggcct
    63541 atccaccgct atcgggaata gagtacagtc cgggagtcgg tgtcgattac tggatatgga
    63601 gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc gttaccattc
    63661 tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc tgggcatcac
    63721 tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc gtcgcgttgt
    63781 tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt ggcaacatga
    63841 tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc ctgatcctgc
    63901 ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt ctgtttggtt
    63961 atacctcgct ggtatgggca accgtctgta tcaccgtgct gtcgttcatc gtttggctgc
    64021 accacttctt tacgatgggt gcgggcgcga acgtaaacgc cttctttggt atcaccacaa
    64081 tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc atgtatcagg
    64141 gccgcatcgt gttccattct gcgatgctgt ggactatcgg ttttatcgtc accttctcgg
    64201 tgggcggtat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt ctacataaca
    64261 gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc ttcggctgct
    64321 tcgcagggat gacctactgg tggcctaaag cgttcggttt caaactgaac gaaacctggg
    64381 gtaaacgcgc gttttggttc tggatcatcg gcttcttcgt tgcctttatg ccactgtacg
    64441 cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg cagttccaca
    64501 ccatgctgat gattgcagcc agcggtgcag tactgattgc gctaggtatt ctctgcctcg
    64561 ttattcagat gtacgtttct attcgtgacc gcgaccagaa ccgtgacctg actggcgacc
    64621 cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc tataactttg
    64681 ccgttgtgcc gcacgttcac gagcgtgatg cattctggga aatgaaagag aaaggcgaag
    64741 cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc ggtgcaggta
    64801 tcgtcattgc ggctttctcc accatcttcg gtttcgccat gatctggcat atctggtggc
    64861 tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc ttcgacgagg
    64921 acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag catttcgatg
    64981 agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc gactgtccac
    65041 gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt ttggatctac
    65101 ctgatgagcg actgcattct gttctctatc ttgtttgcta cctatgccgt tctggtgaac
    65161 ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt tctggttgaa
    65221 actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc catgtacaaa
    65281 aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg cgccggattt
    65341 atcgggatgg aaatctacga attccatcac ctgattgtta acggcatggg tccggatcgc
    65401 agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca cgtcacttcc
    65461 ggtcttatct ggatggcggt gctgatggta caaatcgctc gtcgcggcct gaccagcact
    65521 aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt ggtttggatc
    65581 tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct accgatcaca
    65641 gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg tcgatcattc
    65701 tgacggtgat tccgttctgg atggtgatga caggggctgc ctctccggcc gtaattctgg
    65761 gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc ttcctgcaca
    65821 tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc gtgctaatca
    65881 tcgctatcct agttgtaggc tccatctgga ttatgtggaa cctcaactac aacatgatga
    65941 tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga aaccaggcat
    66001 catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa agggcagcat
    66061 tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg cgtcgggttg
    66121 tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga cgaagaatcg
    66181 ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca ccttgctggg
    66241 tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct ggctgggggt
    66301 gatgggcttt gtggtttatg tcggcgttta tagcctgtac atgaaacgcc actccgtcta
    66361 cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgattggct actgtgcagt
    66421 aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc tgtggcagat
    66481 gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg caaacattcc
    66541 ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc tgtatatcat
    66601 cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctggat ataaatatct
    66661 ggtggtcgcc gcggcggtta gcgtctggtg gttaggtatg gctctgcgcg gttataaagt
    66721 tgctgatgac agaatctggg cgcgcaagct gttcggcttc tctatcatcg ccatcactgc
    66781 cctctcggtg atgatgtccg ttgattttat ggtgccggac tcgcatacgc tgctggctgc
    66841 tgtgtggtaa cacaacctct ctattaaaaa ggtgctacgg cacctttttt cttagcatta
    66901 gaaacatatc cctctcgaaa tatttactaa aaaatccgca tgtttacccc attcgtttgc
    66961 cgctttacac tagtcgcgaa tttaaaacag aggtggtaat gaacgattat aaaatgacgc
    67021 caggtgagcg gcgcgcgacc tggggtttag ggaccgtatt ctcgttgcgc atgctgggca
    67081 tgttcatggt tctgccggtt ctgaccacgt acggcatggc tctgcaaggt gccagcgaag
    67141 cattaatcgg tattgccatt ggtatttatg gtctgactca ggccgttttt cagattccgt
    67201 ttggcctgct ttccgaccgc attggtcgca aaccattaat tgtcggtggg ctggcagtgt
    67261 ttgccgccgg tagcgttatc gctgcgctct ctgactccat ctggggaatt attctgggtc
    67321 gggcgctaca aggctccggt gcgattgccg ctgccgttat ggcgctgctt tccgatctca
    67381 cgcgcgaaca aaaccgcacc aaagcaatgg cgtttatcgg cgtgagcttt ggcattacct
    67441 ttgccattgc gatggtgctt ggcccgatca tcactcacaa acttgggctg cacgcgctgt
    67501 tctggatgat cgctattctg gcaacgaccg gcattgcgtt gaccatttgg gttgtgccca
    67561 acagtagcac tcacgtactt aatcgtgagt ccggaatggt gaaaggcagt ttcagtaagg
    67621 tgctggcgga accgcggctg ctgaaactca actttggcat tatgtgtctg catattttgc
    67681 tgatgtcgac gtttgttgcc ctgcccggac aactggctga tgcggggttc ccggcggctg
    67741 aacactggaa ggtctatctg gcgacaatgc taatcgcctt tggctcggtc gtgcctttca
    67801 ttatctacgc tgaagttaag cgcaaaatga agcaagtctt tgtcttctgc gtcgggttga
    67861 tcgtggttgc ggaaattgtg ttgaggaacg cgcaaacgca gttctggcaa ctggtggtcg
    67921 gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc cctcctgccc tcacttatca
    67981 gtaaagagtc gccagcaggt tacaaaggta cggcgatggg tgtttactcc accagccagt
    68041 ttcttggcgt ggcgattggc ggttcgctgg gcggctggat tgacggcatg tttgacggtc
    68101 agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg gctggcagtc gccagtacca
    68161 tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat cccggcgaac attgccgcaa
    68221 acgaggcgtt aaaagtgcgt ttgctggaaa ctgaaggcat caaagaagtg ttgattgcag
    68281 aagaagaaca ttcagcttat gtgaaaatcg acagcaaagt gacgaatcgc tttgatgttg
    68341 aacaggcaat tcgccaggca taaaagcgag gcgaaattgc ctgctgcgct acgcttatca
    68401 ggcctactaa gttcctgatt tacaaaactt tgtaggccga accaggcgtt catgccgcat
    68461 ctgacatgaa caaagcgcag gttgtcgcga ttaatcgcgg aagtttttga actggaacgg
    68521 ctgaccgaga tcgccaccgc gcaccatcgc catgacagcc tgtaaatcat cacgagattt
    68581 gcccgtcaca cggatctcat cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt
    68641 gatcatcttg acgatttttt tctgagtggc gctctcaatg ccctgtttca gtttcgcttc
    68701 cacaaaccag gttttaccgc tatgaacgat attttccggt acatccagtg aactgccttc
    68761 aatgccgcgc ttcagcagct tggcacgcag aatatccagc aactgattga cctggaagtc
    68821 ggactcgctc aacactttga tggttttgct ggcgtcgttc agctcaaatg aggcttcaac
    68881 gttacggaag tcaaaacggg actccacttc gcggctcgcg ttatcgaccg cgttacgtgc
    68941 ttcctgaaga tcaacttcag agacaatatc gaaagatggc atcttttcct ctcccttcat
    69001 ttttgatgcg aagcataata cctgcaaagt taaataacca acagtcctgc accaggacgt
    69061 atcccttaat tgtgcatagt cgcaacagca tgcacaatta agggatagcc ttattgaaca
    69121 cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac cgtattggga tgcggtgcct
    69181 tagggcaatt atggcttaca gcactttgta aacagggtca cgaagttcag ggctggctgc
    69241 gcgtaccgca accttattgt agtgtgaatc tggttgagac tgatggttcg atatttaacg
    69301 aatcgctgac cgccaacgat cccgattttc ttgccaccag cgatctgctc ctggtgacgc
    69361 tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc gtccacactg cctgtaacta
    69421 cgccaatact gttaattcac aacggcatgg gcaccatcga agaattgcaa aacattcagc
    69481 agccattact gatgggcacc accacccatg ccgcctgccg cgacggcaat gtcattattc
    69541 atgtggcaaa cggtatcacg catattggtc cagcacggca acaggacggc gattacagtt
    69601 atctggcgga tatattgcaa accgtgttgc ctgacgttgc ctggcataac aatattcgcg
    69661 ccgagctgtg gcgcaagctg gcagtcaact gtgtgattaa tccactgact gccatctgga
    69721 attgcccgaa cggtgaatta cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag
    69781 tcgcggcagt gatcgaacgc gaagggcatc atacttcagc ggaagattta cgtgattacg
    69841 tgatgcaggt gattgatgcc acagcggaaa atatctcgtc gatgttgcag gatatccgca
    69901 cgctgcgcca caccgaaatc gactatatca atggttttct cttacgccgc gcccgcgcgc
    69961 atgggattgc cgtaccggaa aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg
    70021 aatatgagcg catcggcact ggtttgcctc gcccctggta gtgaagagac tgaagccgtc
    70081 accacgatcg acctcttgat tcgcggcggt atcaaagtca ccactgccag cgtagccagc
    70141 gatggcaacc tggcgattac ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg
    70201 gtcgaagtgg ctgatggcga atatgacgtg atcgtgctgc ctggtggcat taaaggcgcg
    70261 gagtgttttc gcgatagtac cctgctggtt gaaaccgtta aacagttcca ccgttccggg
    70321 cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt
    70381 ccgattggca atatgaccgg cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg
    70441 caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt
    70501 acagctatcg actttggtct gaaaattatc gacctgctgg ttgggcgcga gaaagcccat
    70561 gaagtggcat cacaactggt gatggcggca gggatttata attattacga gtagtgtcgg
    70621 atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa ccacaaatta cggacgatac
    70681 accttcacat tcttaaagcc ctgttcgcgt agatagagcg cctgcaggcg gctcattacc
    70741 ccgcgctcac accacagtag ccaggttctg ttctggtcga gatcgccaaa tttggtgctc
    70801 aacttgtaga acggcagtga aaccacatca atcccttcga ctttcagtgg cttatcttcc
    70861 tgttcatcga cagaacggat atcgagaatc acgtcgttcg ggccgaagtc attgacggtt
    70921 tccacttcca ccacttcctg cccggtctgc tgggcgattt cgcggatatc aacgttattc
    70981 gcttcttcaa ccactttatc gagaatgctg aagtcgaatt tctcttcttc cgcttcaatc
    71041 ttcgacttaa ccgctttcac cgtcgggctt ttggagatca caccgcagta ttccggcata
    71101 gtacgggcaa agtcttcggt gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg
    71161 taagagatca gcggacgcag gatcagcgta tcagagacgt tatcaatcag gcgcaggttg
    71221 gtcagcgtct gactggacac ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg
    71281 taacgttcag ccactttaga cgcggcacgc accatcatac gtttgaggat aacgcccatc
    71341 tgaccgtcgt cgattttctc gagaatttcc ccgactaccg gctcgaaatt aatagcgaca
    71401 aaacgcacgc ggtgggagct gccaaagcgg ttccacagat aatgtgccac ctgacgcacg
    71461 ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt aatgcacgcg gcagccgcga
    71521 cgcatcaaca tataactgga aacaccggag tcgaaaccac cggaaatgag cgacagcaca
    71581 tcttcctggg tgccgatcgg gaaaccgcca ataccttcgt agcggccttt aatcagcagg
    71641 agacgatcgt cttccacttc cagatggaca gtcacatccg gattggtcag cttcacgcgc
    71701 gcggattcaa tatgctgatt taaaccgccg ccgacgtaac gttccacatc aatcgagcta
    71761 aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg ttttgccatc cagctgatcg
    71821 cgatactgaa ccaacgcttt ctcgaaaata tcgtgcatgt cggtaaacgg cacgtcttcg
    71881 acttcgagaa tatggtggat accaggaata cgggtcaggg cgtcgcgaat agtcagacgc
    71941 tggttttcat ctttcgcgcg aacttcgatg ttatcccagt ggcggacgac agcgagcgtc
    72001 tcatcatagt gctttaaaac gttacgaatg ttcccggtaa ggatttttat aaagcgcaag
    72061 cgcacagatt ggcttttgat ggtgatttcc gggaacaatt taatgataaa cttcatggcg
    72121 gcaatggttc gttggcaagc cttaagcgac ttgtataggg aaaaatacag cagcccacac
    72181 ctgcggctgc atccaggcgc ggaagtatac cactaacatc gctttgctgc gcacatcacc
    72241 ttaccattgc gcgttatttg ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat
    72301 attgagagtc agaaattcat tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag
    72361 gcgctgagcg agctggaaca gattgtaacc cgtctggaaa gtggcgacct gccgctggaa
    72421 gaggcgctga acgagttcga acgcggcgtg cagctggcac gtcaggggca ggccaaatta
    72481 caacaagccg aacagcgcgt acaaattctg ctgtctgaca acgaagacgc ctctctaacc
    72541 ccttttacac cggacaatga gtaatggact ttccgcagca actcgaagcc tgcgttaagc
    72601 aggccaacca ggcgctgagc cgttttatcg ccccactgcc ctttcagaac actcccgtgg
    72661 tcgaaaccat gcagtatggc gcattattag gtggtaagcg cctgcgacct ttcctggttt
    72721 atgccaccgg tcatatgttc ggcgttagca caaacacgct ggacgcaccc gctgccgccg
    72781 tagagtgtat ccacgcttac tcattaattc atgatgattt accggcgatg gatgatgacg
    72841 atctgcgtcg cggcttgccg acctgccatg tgaagtttgg cgaagcgaac gcgattctcg
    72901 ctggcgacgc tttacaaacg ctggcgttct cgattttaag cgatgctgat atgccagaag
    72961 tgtcggatcg cgacagaatt tcgatgattt ctgaactggc gagcgccagc ggtattgccg
    73021 gaatgtgtgg tggtcaggcg ttggatttag acgcggaagg caaacacgta cctctggacg
    73081 cgcttgagcg tattcatcgt cataaaacag gcgcattaat tcgcgccgcc gttcgccttg
    73141 gtgcattaag cgccggagat aaaggacgtc gtgctctacc agtactcgac aagtacgcag
    73201 agagcatcgg ccttgccttc caggttcagg atgacatcct ggatgtggtg ggagatactg
    73261 caacgttggg aaaacgtcag ggtgccgacc agcagcttgg caaaagtacc taccctgcac
    73321 tcctgggtct tgagcaagcc cggaagaaag cccaggatct gatcgacgat gcccgccagt
    73381 cgctgaaaca actggctgaa cagtcgatcg atacttcggc actggaagcg ctagcggact
    73441 acatcatcca gcgtaataaa taaacaataa gtgttaatag gcccctgatg agttttgata
    73501 ttgccaaata cccgaccctg gcactggtcg actccaccca ggagctacga ctgttgccaa
    73561 aagagagttt accgaaactc tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc
    73621 gttccagcgg gcacttcgcc tccgggctgg gcacggtcga actgaccgtg gcgctgcact
    73681 atgtctataa caccccgttt gaccaattga tttgggatgt ggggcatcag gcttatccgc
    73741 ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctac
    73801 acccgttccc gtggcgcggc gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa
    73861 cctccatcag tgccggaatt ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc
    73921 gcaccgtctg tgtgattggc gacggcgcga ttaccgcagg catggcgttt gaagcgatga
    73981 atcacgcggg cgatattcgt cctgatatgc tggtggtcct caacgacaat gaaatgtcga
    74041 tttccgaaaa tgttggcgcg cttaacaacc atctggcgca gctgctttcc ggtaagcttt
    74101 actcttcact gcgcgaaggc gggaaaaaag ttttctctgg cgttccgcca attaaagagc
    74161 tgctcaaacg taccgaagaa catattaaag gcatggtggt gcctggcacg ttgtttgaag
    74221 agctgggctt taactacatc ggcccggttg acggtcacga tgtgctgggg cttatcacca
    74281 cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag
    74341 gtcgtggtta tgaaccggca gaaaaagacc caatcacctt ccacgccgtg cctaaatttg
    74401 atccctccag cggttgtttg ccgaaaagta gcggtggttt accaagctat tcaaaaatct
    74461 ttggcgactg gttgtgcgaa acggcagcga aagataacaa actgatggcg attactccgg
    74521 cgatgcgtga aggttccggc atggtcgagt tttcacgtaa attcccggat cgctattttg
    74581 atgtagcgat tgccgagcaa cacgcggtga cctttgccgc cggtctggcg attggtggct
    74641 acaaaccgat cgtggcaatt tactccacct tcctgcaacg cgcctatgat caggtgctgc
    74701 atgacgtggc aattcaaaaa ctcccggtcc tgttcgccat cgaccgcgcg ggcattgttg
    74761 gtgctgacgg tcaaacccac cagggcgctt ttgatctctc ttacctgcgc tgcataccgg
    74821 aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg ccagatgctc tataccggct
    74881 atcactataa cgatggcccg tcagcggtgc gctacccgcg tggcaacgcg gtcggcgtgg
    74941 aactgacgcc gctggaaaaa ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga
    75001 aactggcgat ccttaacttt ggtacgctga tgccagaagc ggcgaaagtc gccgaatcgc
    75061 tgaacgccac gctggtcgat atgcgttttg tgaaaccgct tgatgaaacg ttaattctgg
    75121 aaatggccgc cagtcatgaa gcactggtta ccgtagaaga aaacgccatt atgggcggtg
    75181 caggcagcgg agtgaacgaa gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca
    75241 ttggcctgcc ggacttcttt attccgcaag gaactcagga agaaatgcgc gccgaactcg
    75301 gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg gctggcataa tccctactcc
    75361 actcctgcta tgcttaagaa attattcatg gcaggagtgg cagcatgcaa tacaacccct
    75421 taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc
    75481 cagatcgtgg taatcacgca tggacactgc cggaagagag cagccgtccc atcattaaac
    75541 gcgcactgga aggcggcata aatttctttg ataccgccaa tagctattcc gatggcagca
    75601 gcgaagagat cgtcggtcgc gcactgcggg atttctcccg tcgtgaagac gtggtcgttg
    75661 ccaccaaagt gttccatcgc gttggtgatt taccggaagg attatcccgt gcacaaattt
    75721 tgcgctctat cgacgacagc ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa
    75781 ttcatcgctg ggattacaac acgccgatcg aagagacgct ggaagccctc aacgacgtgg
    75841 taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat gcacgcttcg cagtttgctc
    75901 aggcactgga actacaaaaa cagcacggct gggcgcagtt tgtcagtatg caggatcact
    75961 acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc gctgtgttat caggaaggcg
    76021 tggcggtcat tccgtggagt ccgctggcgc gggggcgact gacgcgtccg tggggagaaa
    76081 ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa
    76141 atgacgcgca gatcgcagag cggttaacgg gcgtcagtga agaactcggt gcaacacgag
    76201 cacaagttgc gctggcctgg ttgttgagta aaccgggcat tgccgcaccg attatcggaa
    76261 cttcgcggga agaacagctt gatgagctgc tgaacgcggt ggatatcact ttgaagccgg
    76321 aacagattgc tgaactggaa acaccgtata aaccgcatcc ggtcgtagga tttaagtaaa
    76381 tttcgctttt cacccatttt atctgctgaa ttccccatcg tctgcgatga tggggacaac
    76441 gtacctccca tcaccttctg taaccataat ccacctctgc aagagaaatt cttcgtgaat
    76501 gagtttgcag ctattcatcg tcttcatccc tttgagcgat ttttgatagt ttctccaggt
    76561 aatatcccct aaaactgtct tatttagtca acgtaagagg attacaatca tgatgaaaac
    76621 catcaccaaa caaccgatcc tgtttactga tgtaccggtt gcagatttaa gaaactcaat
    76681 gaagcaagat ttaaatcaaa atcttattga aaggctgtgg aacaaaattc gtgatttttt
    76741 tctggacagt gataaacaaa aggcatttaa atcgattcat aaatacatca atacattgtc
    76801 agttctaaat tataattcag cgttaactcc agatcccaac tttaatatag atgcaacgtc
    76861 agatttagac tcatatttaa agctggattt tgatcgcctc tcgcccaaac aaaaacagac
    76921 cacgctttgt tgtttctgga ataaaattgc atcctcattg ccagagccat acaacagtac
    76981 catcaaacat aacattattt tttataaaga cggagaaaat ctgatgatac gtggaactat
    77041 ctctattgtt aacgaggtag taaagactta ttcactccca atagaaaagg atgacaatgg
    77101 atattacgat ttcagcggtc tatatctagc acacagcaat atttccggta aagatcccaa
    77161 taaagatccc gatatagatt tcggtataga tatgggaaac tgtaactgct caaatgtcaa
    77221 cttcgaacat acttattttt atggcgtcaa gtttacaaat gcgaattgta caaatgcgaa
    77281 ttttaacaat tgtagattta agaaatgcga cctgacaaat atgaattgca ctggcgcaat
    77341 tcttgataat gcgatgatat atggtaaaga aaaagaacct gagatgcaat atccagaagc
    77401 agatcaaata atacaaagga taacttatca gaaatcagat gggaatgaaa cgaaagggat
    77461 gattctaact aactgctcct gtgtgaaaac cactttcaat tgggcagatt tatcagaaag
    77521 tgattgccaa aatgtggact ttagcgaggc taatctttca aacactattc ttcctgacat
    77581 cgtcaggatg aaaggcacga aactttaccg taccgacctt tttaatccga tattaaaaac
    77641 agaagcagaa tcaacagaag aaaaagacat tagcccatta gcaaaaatta ttcttgacta
    77701 tattgaatca gataaaaacc ctgagtcatt aaactttgaa gaaaaatcaa cagttattaa
    77761 aataaaacaa gatatcgata attttatttt ctacaatcag catttaaaga aaatattcaa
    77821 tcgcgcaatg aatcttcaag aaaaaataag caggaaaaag tataatgaat tttttaaata
    77881 tatacaagca gaagccaaac aatatttcaa agaccagtat aaactaacaa aaaatgatta
    77941 tttgaagaaa gtacctttaa ctgcacaatt aattgcaaaa tacaaaatgg atgatcaatt
    78001 ggaccaactt ttggttaccc gtgaaattca agatgaaata aaatcaaaga ttcaagacaa
    78061 aatagatgaa ctttctaaaa atttattcaa cactatgacc gaaaccattg agaataattt
    78121 tgatgatatt tttcgtcaac agtcagagaa tatgagcaat tattatgagt tcgtcgactg
    78181 acgatatttc agtgtcattg aattatgagt gacaaagata caccttgaaa tatcgccgca
    78241 tagtatattc ggcgatgtcg ggttttactt taacagagtg ccccaaaatt ggggcactgt
    78301 tcatttataa atcaaaccgg gtggtctgga atcgcctaca cctcccggtc aatggcattt
    78361 aaagaatacc cagcggccag tgatgaccga taaagtacag gatgcctgcg gaaatcaccc
    78421 cggcgacaat atcatcgatc atgatcccca tgccgccatg tacattgcga tcaaaccagc
    78481 ggatcggcca cggcttccac atatccagaa tacggaaaat cacaaacccg gcggcgaccc
    78541 actgccagtc attggtcggc agcgccatca gggtgatcca catgccaata aactcatccc
    78601 atacgatgct accgtgatcg tgcaccccca tgtctttcgc cgtttgatga caaagataga
    78661 cgccgataca gatccccaac atcaccacca gcgagtagag ctgccagggc aaaaaggtca
    78721 tcagatacca gaacggaatc gccgccagcg atcccatcgt gccaggaacg attgggctta
    78781 atccacttcc gaatccgaca gcaagcagat gccacggatt actcatcttc aggcgacttt
    78841 tcgcgacatc tttatggcgt ggcaaaatgg tcatatcctt tccagtctaa cgtgacaggt
    78901 tcgccgtcac gaataaaaca aagcccttcg atatcggcgg tcatttgccc gatacaggta
    78961 aacggtacgc ccagatgacc gagagccaca tccagcgcgc cacggttcag ttccggcacc
    79021 gtgaaacaca actcgtaatc ttcaccgcca gagagcgccc agcgcagcgc ctgttccggt
    79081 acaacatggc gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat acgtgcgccg
    79141 cagtcgctgg ctttcacgat atgcccgaga tcggaaatca gaccgtcaga gagatcgata
    79201 gctgaatttg ccagatcgcg cagtgcctgc ccctgtaaaa tacgcggcga tggacggaga
    79261 tgacgtttga tcaagtagtc cgcatcttta gcatcggcaa cctgcaaacg gttttgcaaa
    79321 atcgccagcc cggcggcgct atcgcccggt gtaccggtca catagatcca gtcacccggt
    79381 ttcgccccag agcgcgttaa ggctcgtccc atcggaacaa agccgtggat acccaacgtc
    79441 attgataatg gcccacgcgt ggtatcgccg ccaatgagtt gcatatcgta ataattgaga
    79501 agatcaaaca aactgtcgct gaaggactca agccacgctt cgtctacgtc cggtaaggtt
    79561 aatgccagcg tcagccaggc cggatcggcc cccatcgctg ccagatcgct taggttcacc
    79621 gccagtgctt tataagccag atcagcagga tcgatatcag ggaggaaatg gttgcccgcc
    79681 accagcgtat cagtgctgat cgccagggtc tgtttctcgg ggatattgag aagagcgcaa
    79741 tcgtcgccga tgcccagttc gacatcaaga cgagaacttc ttacacggtc aaaataacgg
    79801 gcaatcaggg agaactcgcc acatgccata cgttatgcct cagcaggtaa aaaagaaaag
    79861 gccggcgatc gcggaacagt cttccgtgaa tctaccggcc tggatattac tttttgttag
    79921 ggcgaatcac aggtgctgct ttatcgagta cgccgttgac gaacttatgg ctgtcttctg
    79981 cgccgaacga tttcgccagt tcgatcgctt cgttaatggc cactttgtat ggcacatcgc
    80041 tacgtttaga cagttcgtac agcgcaatgc gcagtactgc tttttctacc tgtcccagtt
    80101 cttccagcag gcgggacagg tatggcttca tcagtccgtc gaggtatgcg gtattagtcg
    80161 ccaccccggc cagcagctca cggaagtaca ggacgtcaac gtcttttaca tcctgttcag
    80221 ccaggaactg gtattcaaca tcagcgatgt cgttctggga caactgccag gagtagagcg
    80281 cctggacggc acactcacga gcgcggcgac gagcagcagg tttcacggat ttccccttac
    80341 taatttcagg ccttgatggc tttcaataca ttaatcattt caagcgcggt cagtgcagct
    80401 tctgcacctt tgttgccagc tttggtgcca gcacgttcga tcgcttgttc aatgctttca
    80461 gtggtcagaa ccccaaaagc aaccggaatt tcgctgtcct gggcaacatg cgccaggccg
    80521 ttgcttgcac caccagcgac atattcaaaa tgggcagtgc caccacgaat aaccgtaccc
    80581 agcgcaatca ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc cagcggcagc
    80641 tcataggcac caggcaccca aacaacggta atgttttcat cttttacctg accgatacgt
    80701 ttcagtgcat caattgcacc ttccagcagg ctgtcattga taaagttgtt gaaacgcgcg
    80761 atggtgatgg cgacgcgagc gtccggggta gcaacgttag cttcaataat gttcattttc
    80821 ttccttcggg ttcgagtatg gccccgcagg ggggcggatt ttagcataat atttcgtgcg
    80881 ctgcttccct ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa catccgggcc
    80941 tacatgacgt atctctttga atttaaattg gggggcgtca gctaatttct caagccctgg
    81001 cagtgtgcat aatccacggg cgtcgctgcc taatagttta ggtgcgatat agacaatcag
    81061 ctcatcgact aaccccgcct gcagcaatgc gccagcgagc gttggccccg cttccaccca
    81121 gatgctgtta atctgctgtt tacccagttg catcatcagt acaaccagat ccagatgacc
    81181 tttatgctct ggaatcagca aggtacgcac cgtttccggc cactcacgag aatcttcctg
    81241 cgtacgcgcg aaccaggttt cgccgggctg ctgcacaatg tgatgttccg gcgtcacgcg
    81301 attttggcta tcaatcacaa tacgtaccgg ctggcggaga tgatgttgcg ggtagagcgc
    81361 ctgagtttgt acatccagtt cagaccaacg caccgttaag gcaggatcat ccgccagcac
    81421 cgtagcgctg ctggttaaaa tggcatgact ttgtgcgcgc agtcgttgta cgtcgcgccg
    81481 cgcctgaggc gaagtgatcc actggctttc gccgctcgcc atcgccgtgc ggccatcaag
    81541 cgatgcgcca agttttaact gaatataagg aaagccggtg cgcatccgct tgagaaagcc
    81601 tttattcaat tgctcggctt cactcatcat caggccgtgg ctgacgtcaa tgccagcctg
    81661 ttgcagacgg taaagtccac gcccagcgac ctgcgggttc gggtcctgca tcgcagcgac
    81721 cacgcgcgct acgccagcgg cgatgagtgc gtcacagcac ggtggcgtac gaccatgatg
    81781 gctacagggt tcgagtgtga cataggcggt cgcacctttg gctttttcac ccgccatacg
    81841 caacgcgtgt acttcggcat gtggttcacc cgctcgttgg tggtaacctt caccgacaat
    81901 ttcgccatct ttgacaatga cgcacccgac attcgggttg gggtgggtgg taaaacgtcc
    81961 tcgttgcgcc agctttagcg cccgcgccat gtaatactcg tcctgcacgg cttagtcctc
    82021 caggcgcgcg atctcttcgc caaattcttt gatatcttcg aaactgcggt agacagaggc
    82081 aaaacggata taggcgactt tatcgagctt tttcaattgc tccatcacca gattgccaat
    82141 catcttgctc ggcacttcgc gctcaccggt ggcgcgcagc tgcgatttaa tatgattgat
    82201 tgccatttcg acgtcatcgg aactcaccgg acgtttttcc agcgcccgca gcattccgct
    82261 acgtaatttt tcttcattaa acggttcacg cacgtcgttg ctttttacaa cacgcggcat
    82321 aaccagctcc gccacttcaa aggtggtgaa acgttcatta cacaccagac actgccgacg
    82381 gcggcgtacg gatgaaccct cgcccacgag acgagagtca attaccttag tgtccacggc
    82441 gaaacagaat gggcaatgca tacggcgtcc tgaccaggtg gttaacggaa atctatttta
    82501 ccctgaactg acgatacaac aaaggcgcaa cgcttttgag ataaaggatc gatgctttcg
    82561 ctgcttttac cctctaccat taggctaatt gcgacgagaa agggacgacc tatgaatatt
    82621 aacgtttttc gactgatcct gctgggaagc ctattcagcc ttagcgcctg tgtgcagcaa
    82681 agtgaagtgc gacagatgaa acacagcgtc agcacgctga accaggagat gacgcaactc
    82741 aatcaagaaa cagtcaaaat cactcagcaa aacaggctga atgcaaaatc cagcagtggg
    82801 gtttaccttc tgcctggagc gaaaacaccg gcaagactgg aaagccagat cggtacttta
    82861 cgtatgtcgc tggtgaatat tacgcctgat acagatggca ccaccctgac actacgtatt
    82921 cagggtgagt cgaatgatcc cttgcccgca ttcagcggaa ctgttgaata tgggcaaatt
    82981 cagggaacaa tagacaactt tcaggaaatc aatgtacaga atcaattgat taatgcccct
    83041 gccagcgtcc tcgcccccag cgatgttgat attccgttac agttaaaggg gatttctgtg
    83101 gatcagttag gctttgtacg tatccacgac attcaacctg ttatgcagta aacttatagc
    83161 ggggcgattt tgtgcgtccc gcaacatcct tccccatcat tttgttactc cgcttacata
    83221 acccagattg atagtaaaag tttgcaacaa gggcgaaagt cagtacaatc cccgcccgaa
    83281 tgtgtgtaaa cgtgaacgca atcgattacg taaatgatag aactgtgaaa cgaaacatat
    83341 ttttgtgagc aatgattttt ataataggct cctctgtata cgaaatattt agaaacgcaa
    83401 tttgcgcctt tttcactccc gcaagggatt ttcaaacagt ggcatacata tgaaaaaaac
    83461 attactggca gccggtgcgg tactggcgct ctcttcgtct tttactgtca acgcagctga
    83521 aaacgacaaa ccgcagtatc tttccgactg gtggcaccag agcgttaacg ttgtcggaag
    83581 ctatcacacc cgtttcggac cgcagatccg caacgatacc taccttgagt acgaagcatt
    83641 cgctaaaaaa gactggttcg acttctatgg ttatgcggat gcgccggtat tcttcggcgg
    83701 taactccgat gcaaaaggta tctggaacca cggttctcct ctgtttatgg aaatcgaacc
    83761 acgtttctcc atcgacaagc tgaccaatac tgaccttagc ttcggtccgt tcaaagagtg
    83821 gtacttcgcg aacaactaca tttacgacat gggtcgtaat aaagatggtc gccagagcac
    83881 ctggtacatg ggtctgggta ccgatatcga cactggcctg ccgatgagcc tgtccatgaa
    83941 cgtctatgcg aaataccagt ggcagaacta tggcgcagcg aacgaaaacg agtgggacgg
    84001 ttaccgtttc aaaattaaat actttgtgcc gattaccgat ctgtggggcg gtcagctgag
    84061 ctacatcggc ttcaccaact tcgactgggg ttccgattta ggggatgaca gcggtaacgc
    84121 aatcaacggt attaagaccc gtactaataa ctctatcgct tccagccata ttctggctct
    84181 gaactacgat cactggcact actctgtcgt agctcgttac tggcacgacg gtggtcagtg
    84241 gaacgacgat gcagaactga acttcggcaa cggcaacttc aacgttcgct ctaccggctg
    84301 gggtggttac ctggtagtag gttacaactt ctgattatga aaatgccggg atttattccc
    84361 ggcatttcag attgttaaca acgtacgctt tgttcatgcc ggatgcggcg taaacgcttt
    84421 gtccggccca caaaagcacg taaattcaat atattgctga aataacgtag gcctgataag
    84481 cgtagcgcat caggcaattt tacgttttaa tcacttcttc ttgttcatca tcgctttcag
    84541 gtcagcgaat gggttgtact tcgcttcacc gacatctttc tgcgcatctt cccctgcgat
    84601 aacggtcgta ccatactgat ccgcttcggt atatttcgaa tgctcatgat cgtggcaata
    84661 gagacaaagc aattcccagt tactgccatc ttccgggtta ttggtatggt cgtgatcaat
    84721 gtggtgaacg gtaagttcac gcaggttgga ataaacaaac tcgcgggaac agcgaccgca
    84781 gacccacgga tagattttta atgctttttc gcgatagccg ctttctaacc gcgcatagtt
    84841 ttttgggatg atagccatga aaagttacct ggatgaaatg tagggttacg cgcagtttcc
    84901 caagatgcgg ggattttcgc aatcctgagt caagaaattc tcacattacc agtgacggcg
    84961 aggttccatc aactgatgaa gaatgcgcgc aatgagaatg tcatcgactc ttataacgta
    85021 ataaacagtg tgaaacctaa atgcgatctt ctttacctct ggaatcgcat catagtcttg
    85081 ccctatctcg ggcatttgag agagtgtatc cagaaaagtg ccgagagagt cggtatagcg
    85141 atccgcctgc tcatcaccaa actttcccag cgtgtagtca tagatccctc tgaaatcttc
    85201 aacagctttt ccgctaagct tatacacgat gtttttgctt cctttctgct gcgatatcac
    85261 gaagcgtcat cgaactttca ccgctctcaa gaccttcaat aacagcattt ctgacagctt
    85321 catccctgga ttcttgttgt tccaatagac gaagtgccga acgcattact tcactggccg
    85381 agccataacg accagactgg accatccggg taataaaact atctaattgt tcgccaacgc
    85441 ttactgaggt aattttgcgc ataagcgatc tccacgtatt aactattaat acaaagtata
    85501 tgtcagaacg ctttctacaa ccaaaccgta ttcagttgcc tgtgaaacag gcaaaaaaaa
    85561 accgcagcgc aaatacgcca cggttttttt aacagaacgg atttaaaaca ccattacggc
    85621 agaattgacg gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc
    85681 ttcataccca gtttcagagc caacgcagat gccacataga tggaagatgc agtaccgatg
    85741 gaaacaccga taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac
    85801 agcatcagga taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg
    85861 gtcaaggaca cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca
    85921 cgaattcggt ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac
    85981 gatgccacaa tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg
    86041 ataacgtcgt gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa
    86101 cctacgtaca cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca
    86161 aggtctgccc ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg
    86221 gtggattcgt taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct
    86281 tcagcaggcg gcatacggac catgatgtcg tggctgctac caaagttttg cagcatcggc
    86341 tcttcaaaac cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg
    86401 agcgtaattt cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg
    86461 cccataataa cgatagcagc gattaacagc agaccagaga tgccaaaagc ccagtagtcc
    86521 cagcgcataa agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt
    86581 gccacatcgc actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt
    86641 tacgatggca cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt
    86701 agtaatcgcg aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat
    86761 cagcgtggtg atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat
    86821 tgcctgttga acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac
    86881 gttcgcatcg accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag
    86941 tgttgcgcct ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc
    87001 actggtcgca atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag
    87061 cagaccggca aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc
    87121 aattgtacgt tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag
    87181 tgacagctga cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag
    87241 acgagactgg atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc
    87301 atttgcatct ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc
    87361 gatgttgtcc ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt
    87421 aacctgcggc tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt
    87481 aagaattacg cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga
    87541 gtcgcccggt acgcgaccgg atgccgcagc ggcctgatca acgttggtgt ttaccagacg
    87601 gaattccagc gttgccgtcg cacccagaat ctctttcgca cgcgcagtgt cctgaatacc
    87661 tggcagttca acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc
    87721 aagttggttt acacggttac gcaggatatt gatgttctgc tgcaccgcat attcacgcgc
    87781 ttcactcaga cgagcatcgc tcattaccgc acgcagctgg ttgctgccct gactgctaat
    87841 caccaggtcc ggatgacgct tgctcagata cgcaatggct tcatcacgag ctttagcatc
    87901 gcggaaagtg atgctcaggc cgtagttatt ttctttacga acggtggtat acgggatgcc
    87961 tttttcgcgc aggtcgctgc gcaggctatc gatattttgt tcctggagtt tgccaagcgc
    88021 ggtgtccata tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt
    88081 catcggctca gcgtgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc
    88141 cacgacgtat ttgtcaccca taacgcccat taatgcttca cgagcgcgca gctgggtgtc
    88201 agtggagtcg aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat
    88261 tttttcttct tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc
    88321 gcgcgcacca gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag
    88381 cagaccgatg acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa
    88441 cacggcaatt cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag
    88501 gacggcagct acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc
    88561 aatgtagccg ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac
    88621 ttcatcacct ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg
    88681 tggacgcagg atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga
    88741 catcgggcta ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct
    88801 cattaaattt ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct
    88861 gacgctggta aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta
    88921 aacccgccat caaacgctgg tagtaacgaa ggttatgaat ggtattgagt cgcgcgccta
    88981 atatttcgtt gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt
    89041 agcaatcaca ctcaggatcg agtgggccag tatcgctctt atacttcgca ttgcggattt
    89101 tcaccacgcc atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc
    89161 agtcaaacat atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc
    89221 ccatcaggta acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt
    89281 gcatatctgc tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga
    89341 tatctaccag acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga
    89401 taccaaacag cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc
    89461 aacgcagaga catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac
    89521 actcatcaaa gatcatgacg atatccgaac caagatcgta ctgaatctcc atcgactttt
    89581 ccgggtcgag gaaaatcggg tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg
    89641 tgattttacg aatatcgcca aggctgaaga cttggaagcc gccggagtcg gtgaggatcg
    89701 gtcccttcca ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg
    89761 ggcgcagcca caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt
    89821 cttccggcgt catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt
    89881 ccactacgcc acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca
    89941 gttcaaattt cattttttct ccactacgtc agaaaaacag tccaacgttt aaaccagcgc
    90001 cgcggaatta ctccccgacg cgctcattaa ttgcctgcgg attgtacgtg ataaacatcg
    90061 catcaccgta actaaaaaag cgatatttct cttctaccgc cgctttatag gcattcattg
    90121 tgtgttgata accggcaaag gccgaaacca gcataatcag cgtcgattcc ggaaggtgga
    90181 agtttgtcac cagcgcatcg accactttgt actggaagcc cggatagata aagatttggg
    90241 tatcgtcaaa gaacggttcg atgagatcgt ttttcgccgc ctgagccgcg ctttccagcg
    90301 aacgtactga ggtggtgcca accgcaatca cccggttacc gcgcgctttc gccgccagta
    90361 ccgcgtctac cacatcctgc ggtacttcag cgtattccga gtgcatgatg tgatcttcaa
    90421 tggtgtcgac gcgtaccggc tggaaggtgc ccgcaccaac gtgcagggtg acgaacgcca
    90481 tctccacgcc tttggcgcgt aatttttcca gcaaaggttc gtcaaaatgc agaccagcgg
    90541 tcggggctgc aaccgcgccc ggtttttcgc tataaacggt ttgataaagt tcgcggtcag
    90601 cgtcttcgtc cggacggtcg atatacggcg gcagcggcat atggccgatg ctgttgagaa
    90661 tatccagcac cgagcgttca tcattaaatt cgacttcaaa cagtgcgccg tggcgcgcgg
    90721 tcattgttgc gttaatactt tcgtcatcgc ccagcagcag ttctgcgcca ggttttggcg
    90781 ctttcgaggc gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgt tcaaccagca
    90841 cttcaatctt gccgccgctg gctttacgcc caaacaggcg cgccgggatc acccgggtat
    90901 tattaaaaac cagaagatcg ccggggttga gcttatcgag taaatcggtg aaagtaccgt
    90961 gcgtcagcgc gcccgtcggc ccgtccagcg acagtaaacg acagctactg cgttcaggca
    91021 tcggatagtg ggcaatcagg gattcgggca attcaaagga gaaatcggta acgcgcatga
    91081 cactgactca gactaaaata agaggcgggt agtctagtgc cggggcgctc tccctgcaac
    91141 ctttaccccc ctttggatag aatataatcg atgaattttt tagctcacct gcatttagcc
    91201 catctcgcgg aaagctcgct ttccggcaat ttgctggctg atttcgtacg cggaaatcct
    91261 gaagaaagtt ttccgcccga cgtcgtggct ggcattcata tgcatcgacg tatcgacgta
    91321 ttgactgaca atctgccgga agtccgcgaa gcacgggagt ggtttcgtag tgaaactcgc
    91381 cgcgttgcgc ctattacgct ggatgtcatg tgggatcact ttctttcccg tcactggtcg
    91441 cagctgtcgc cggactttcc gctacaggaa tttgtttgtt atgcccgcga gcaagtgatg
    91501 acaattttgc cggactcacc gccacgcttt atcaacctga acaactactt atggtcagag
    91561 cagtggctgg tgcgctatcg cgatatggat ttcatccaga acgtgttaaa cggcatggca
    91621 agccgtcgcc cacgtctgga tgccctgcgt gactcctggt acgatttaga cgcccattat
    91681 gccgccctcg aaacccgctt ctggcagttt tatccacgga tgatggcgca ggcgtcacgc
    91741 aaggcgctct gaaacttcaa actccggcgt cactattttt tagcattcgc atccagcggg
    91801 gcggcataac aacggtaaga aaaacattcg ctggaatgat tggcgatatc tttaaagttg
    91861 ctttcagcaa ccccctcgaa catgagtacc gttctggaca actctccgtt ttcatttata
    91921 tgtggaaaat caaattggct actgaatcgc catacatctg tcttttctgg ctcgctcttc
    91981 acagagatat gtcgatggag gccccctcct gtaaacgtgc gccagaacaa ttgttgatca
    92041 aaatcttgta atatcgcaga cttggatgct atttgacgta gtaaacctgc ataccgtgga
    92101 ctgccattac gagcaacatt atgcaaaccc tgactaaaag catcaaacaa acaatagatg
    92161 tgatcactag tgaaaaaagg tcttaatttt ttttgttctt catcgctgta atccaggagc
    92221 atctcattga tttcttccgg tgagaatttt aagacaaaat tatcctcaga atttgtttgt
    92281 ttcttgtttt tttcaatatt cttctcctca atgctcttcg cattttgttt cgcaattatt
    92341 tccgcagttt ttttcgcaat ttcttccgca attttttccc gagttattga ttttagacta
    92401 acaccatcga tgtcaaccgg tgttgttttc agatctctct gcgcgtcttc caaatttgtc
    92461 ttatagagtt tgtgcagttt atcggcagat acgtatccgg atgaagaaat ctggtgtgta
    92521 cgcatggggg gctgttcaca aagcatcgag gctatatcgt ctttaaactc ttttacatta
    92581 aagttctcgg ctttaacctt ccttgttata aaccccgcat cggcaatttt cttctctaat
    92641 tgttttgtac gtttagaata ataagcgttg ttatcataaa acttttcaag aagggcccac
    92701 tcctcaagtt gccttatgtg atttttcaac tgttgatgat ctattttata ttcatgtgta
    92761 ctagaatcat tttggttgca ggagttacag atttttaagc ataattttcc atcagattta
    92821 tcatctatgg tatcagtgaa aatataggac acttcaatac tctgcccgtt cttatcagtc
    92881 atctcatatg accattgtcc cgttcgtttc gcattcttaa tgacttcgat cgactctaca
    92941 ggacaaccat gaagaatggg cagaatttta tcaggatgct taatgagtaa aattattata
    93001 tttacaatgt ttgaaaagaa atttttgcta ttttttattt catcgatttt ttccgctgaa
    93061 atgtttacta agttttcatt tccggaaatg aaaagcgatt tgccataggt gcttttttta
    93121 aattgttctt ctgttttaga aaagacatcc tctttaaaag gtggcaactc agtaccagta
    93181 ataaacggtt tcaataaatt cgaatttgaa attgtcatat ctttgctcca ctacagggtt
    93241 atcctggagc atgttgtaca taacatcatg ggcaaacaat accaaaaatc gctcatatcg
    93301 atcgggacaa atatttaaca aattcagccc tgacattgag cagctttttt gcgctgcgta
    93361 gtgtcagggc tggtgactaa cgacgtaaca aataaaacag atgctttagt tcatccacac
    93421 cgtagccgaa atggcaggca aagccagaat cccgtctgtc agttgcccat ggccttcttt
    93481 gcgttgccat tgcacgacat taagaaacgg tgacgcgggt agcaccactt cacaggcttc
    93541 gccacggtta attgccacca gtacacgctg ctgattcagc acgcggacaa ataccaccac
    93601 gttatcttcc gcatacagca cctgacagcc gccacgacgc agcgcctgac ttttcttacg
    93661 cagcgcaatc attcgctggt acaacgcgaa taacgccata tcctgttttt ccacctgcca
    93721 ggggaacggc ttacggcaaa acggatcatt tttgccatcc agtcccactt catcaccgta
    93781 ataaatgcac ggtacaccag gccaggtgaa cagccagacc actgccaggg gcaggcgcgc
    93841 aacctcccga ccgagcagcg ttttaaatcg cgcagtatcg tggctgtcga gctgattaaa
    93901 catacgtaat tgctgttgat gagaaagccc tgcgcggtaa ttatccatcc aggccataca
    93961 ggtttgagcg tcaatttgct gcggatcgta agagatgtcg gtattggcaa gaaatcccca
    94021 caacgggaat gtgaagccgc gatagttcat ggcggcatct tccacatcgg cctgtaacca
    94081 ttgccgtgca tcgccaaaat gctctccaac tatatacgct tccggctggg tttctttcgc
    94141 cgcttcggtg atcccagcaa cgtgctgcaa attattgcgc gccccacccg cttcccccag
    94201 catatgcacc acatccagcc gccagccgtc catattccac ggcgctttca gccagtggcg
    94261 gacaatactg tcttccccgc gataaatttc attcaccaga ctttccgact gataatccag
    94321 cttcggcaag ctggcatagc caagccagtc gagcgccgtg ccatcatcac taaacgagta
    94381 ccagtcgcgc cagggcgatt cggggttgtg acaagctccc cccgtgccac gattgtgcct
    94441 gtcaaaccag gcatgggaat cgccactgtg gttaaacacg ccatccagca ccagccgcat
    94501 tcccagctgc tgcgtattgt ggcgtaagcg cagcaacgcc ccatcaccgc caaactgcgg
    94561 atcgacatgg cgataatcct cggtatcgta tttatgtacg ctgggagctt taaacaccgg
    94621 attgagatac agcgccgtca cgccaagctt tttcagatac ggcagttttt cgcttatccc
    94681 gtccagatcg ccgccataga acgttgatcc gcccgcctgc gccgtgaccg gttcatccca
    94741 gtcacgcaag atgatctctt gtccggctgc atgatggtaa tagacatgat cctgttcagc
    94801 ttcacgagga agactacgcg caaaacgatc agggaagatc tgataaaaaa tctgatccgc
    94861 agcccattgt gggccgatat ccggtacatc gacggcaaac tgctccagtc gtgccggcgg
    94921 cattcggctg aagccctgcg gtgtaaacca acgctggcga tcgtgccaca gcagtttgaa
    94981 actgtaacgc cgccggggtt gtccgctgga gagatcaatc gccgcacgcc atgcggtgac
    95041 gcctggctgc ggctgactgc gctgcttatg cattggtact gacatttctt cgttatcgtg
    95101 ttctgtacgc agcataatgc gctgcggtgg gtcttcgccc gtcagccaca gtgtaatgag
    95161 cagttgatct ttgctttgtt taacaaatgg gggcaccggc aggtgccatg catttaacat
    95221 cataattccc ctgtgatgaa aatctgatca ccttgccata gcctaatgca ggatcactca
    95281 tcatctgaag gatatttggg ggaggaggac gggggcggat aactttcgga atagccggat
    95341 caggatttcc tcctgaaccg gcttacagaa cgcaagtatt attggttttc agccagctga
    95401 cgatcgtggc gacgcttaaa catccagcca atcaacagca caacaatcca cgcgaaaccg
    95461 acatacagcg agatacgcgt atccgggtga taaccaatca acccgataat aaagagcagg
    95521 aaaatcagcc cgccgatggt cgttgctacc ccacccggca ctttaaattt cagcgcctta
    95581 acttcttctg gcggcaaacg gcgacggaag gcaatttgcg acagcaggat cataatccac
    95641 acccacaccg tggcgaaggt tgccagcgaa gcaatcacca ggaagacgtt ttccggcatg
    95701 atgtagttca gatacaccgc aaacagcagc gcggtagtca tcaccagcac cgtaacccac
    95761 ggaataccgc gacgcgacgt tttgctgaaa attttcggcg cgctgccctg ctctgccata
    95821 ccgtggagca tacggcctac gccaaataca tcactgttaa ttgccgacag cgaagcagtc
    95881 agcacaacaa agttaagaat gctggcggca aaggtaatgc ccatatgctg gaacgtcagc
    95941 acgaacgggc taccggcagt gccaacctga ttccacgggt agatagacat aatgacgaac
    96001 agcgtaccga cgtagaacac cagaatacgc atcggcacgg agttaatcgc acgcggtatc
    96061 gatttctcag gatctttcgc ttcaccggcg gtaatcccga taatttcgat cccaccgtaa
    96121 gcaaacatca ccatttgcaa cgacattacc atgccaagcc agccgttact gaagaagccg
    96181 ccgttgctcc acaggttatg aataccggtc ggttgcccgc cgttgccaat cccccagatg
    96241 atgatgccga aaccggcgac aatcatgatg atgatggtgg cgactttaaa gaacgagaac
    96301 cagaattcca gctcaccgaa taccttcacg ctcatcaggt ttacggcgca aatgatcagc
    96361 accacgctca gtacccaaat ccagtgcggc accgtcggga accagacacc catatagata
    96421 ccaaaagcgg tcacatcggc gatggcgaca ataaggattt caaagcagta ggtccagccg
    96481 gtaatgtaac ctgccagcgg gccgaggttt tcctgcgcat aacgcgagaa agagctggcg
    96541 gccgggttat gtaccgacat ttcccccagc gcacgcataa tgatatacgc cgcgatacca
    96601 ccgataatat aggccaacaa cacgctcgga ccggccattt tgatggcgtc tgccgaaccg
    96661 taaaacagcc cggtgccaat tgctgaaccc agtgccataa agcgtatgtg gcgggtactt
    96721 agcccacgct ttagcttgtt cttactttcc atcaattcct aacccatcaa cacaataaaa
    96781 aaccacgggg catcgcagcc ccgtggttaa aacaaatgtt cagtgattta gtgagcgctg
    96841 gaggtcacct gacgacctgc cgcgcgatcc cagataatgg ccagaaccac catcaccact
    96901 gttggcatta accacgccag accttgttcg gccagcggta aacgctgcgc ccaggacggt
    96961 aagatatcgc tgaatgcaga tgctttgatc ccgtcgagaa taccaaaaag caggctgata
    97021 aacatcggcg gagcaatcac gcgggacgaa ttatgccacc atgagcgtgt aaaacttaat
    97081 acaaccagtg cgatacacgg cggataaata gcggtcagca ccggtacgga gatctggatc
    97141 agctggctta agccgaggtt agaaaccacc atcgagaagc cgccgaggat aaacaccagc
    97201 gtacgataag agagcggtac gtactgggca aagaattctg cacaagcaca ggtcaggcca
    97261 actgccgtta ccaggcaggc gatgaagatt aacgccgcca gcaggaagct accgccgccg
    97321 ccaaaggtgt gctgaacgta agcatgcaga atagcagcgc cgtttgcaga ctgatcgacc
    97381 agcgacgcgc tgtctgaacc cagacggaac agcgccaggt acagcagagt cagaccaaca
    97441 cccgccatca ggccagccca gacggtataa cgggtcagta ggcgcgcttc ggtaacgcca
    97501 cgagaacgcg ccgcgttaac aataacgata ccaaacacca ttgcgcccag cgtatccatg
    97561 gtcagatagc cgttgacgaa gccgttagaa aacgcagcgt tttgataagc ctcagtcgcc
    97621 gtgctgatag aacccgccgg ccagacaatc gcggcaacag acaggatgac cagcgcgata
    97681 attttcagcg gcgcaaggaa gttgcccacg gtatccagca gcttgcccgg atagagcgaa
    97741 accagaataa cgatagcgaa atagaccagg ctgtagataa acagcggcag cgcggaatca
    97801 cccgtcagcg gcgcaatccc cacttcaaaa gaaacggtag ctgtacgcgg cgtagcgaaa
    97861 agcggcccca ccgccaggta acaaaccgtt gccagcagta cgccagcgac tttaccgatt
    97921 ggcgtgctga ggctgtcaac accgccgcca acttttgcca gcgccactac cgttaatacc
    97981 ggcaggccaa cggcagtaat gaggaagccg aatgccgcag tccagacgtg ttcgcctgcc
    98041 tgtaagccga ccattggagg gaaaataatg ttacctgcgc cgacgaacaa cgcaaatgtc
    98101 ataaagccca gagcgatgat atcgcgcgat cttaattgat gggtcataaa tcatactgcc
    98161 tgtggatgtg gtgttgtaac gttaaatttt tgtgccaatc cctggggatg atacagcggt
    98221 atatttaaac aattttagcg ggaaatactc ccaaccaggc gcggcgaaga atagtttttc
    98281 gatttaccgc gttaatgcag tcagtatgac agcacctgaa gcgcaattta aacgcttata
    98341 acgttttaaa gcaaggaggg atctaaaaac cagaggtata tagcgataag aaatcaccaa
    98401 ccagtataaa acactatttc cgtgaaaaag acatggctaa tcatgcgaac aaaaaatcac
    98461 caaccgttgt aaaatctaac gcccgtattt tgactgcaaa gaaaataagc cagcatcggc
    98521 tggcttatgg caataaaaga tgacaaaggc ggattaatcg ctgtttttga caattaaacg
    98581 ttccgggata acaaaactga aacgtgttcc ttttcctact gtactctcaa tattcaggcg
    98641 actttcgtga tgattcaccg catgtttcac gatcgctaac cctaatccgc taccgccggt
    98701 ttgccgggaa cgcgctttat caacgcgata aaaacgctcg gtcaggcgcg gaatatgctc
    98761 cggtgcaatg cccggtccgt tatcttcaac gctaaattcg gcaccgtgcg gcactcgctg
    98821 ccagcgtacg gtgatatgcg tgccttccgg cgtatgattc acggcgttat agaccaggtt
    98881 cgaaatcaca ctgcgtagct gatcttcgtt gccagacacc ttgaggccgt tatctatctc
    98941 gaaggtaaat gtctgttttt tctgactcag agtctgagcc tcgcgctcaa caacgcgcag
    99001 catcatcggc acatcaacct tttcattgag caattgcgtc ggtgcggctt caattttcga
    99061 cagcgttagc aactgcttca ccagcccttc catccgctgg gtctgctcgc gcatggtgtg
    99121 caacgctttt tcgcgtaccg cgccttccag cggctgctca tccatcatct ccaggtaacc
    99181 ctgtaacacg gtcaatggcg tacgtaactc atggctcacg ttggcaaaaa agttacgccg
    99241 cgccccttcc agttgatgca tttgcgtgac atcacgcgcc accatcagca actgtttgtg
    99301 ggtataaggc atgacgcgaa tttccagatg ccgcccggtg ttgagcacca gattgagcgg
    99361 gcgagaaaaa tcacgcgttt tcaaatattg cgtaaactcc gggtaacgca gcaggttaag
    99421 gatgttctgc ccgttatctt ccggccagcg caaaccaaga atctgttgcg ccagaccgtt
    99481 acaccagaaa ataccgccct cttccgtggt cagcaccacc gcgtcgggca gcgactccgc
    99541 gccgctacga aagcgtttaa tcagattgcc cagttcacgg cggcgttttt tatttcgcag
    99601 ctgcatctgg tgtaagccgt atagtagcgg ttcccagcta ccacgccccg gtggcggggt
    99661 catactgcga tccacccaca gccaccatga aaggcgcaat aaattccaga aatgccagat
    99721 aagcagtcct gttaccgatg ccagcaaaaa ccagggcagg taaccaaaaa atgcacccag
    99781 gatgaaagcc gggaggcagc aaagtagcag ctccagcacc agccttttcc acgacagccg
    99841 ttccagcacg taagatactc cagttaagaa atcataagcc ctgctctgcg tccgatgagc
    99901 aaggcgttaa aagcgggttg aaaaacgata tcctgtaccg cgcacggtct gcaccatgcg
    99961 gtcatgcccg ccgggctcca gtgctttacg caggcgacga atgtggacat cgaccgtgcg
   100021 gtcttccaca tacacgttag ttccccagac gtggtttaac aactgctcgc ggctgtacac
   100081 gcgctcagga tgcgtcataa aaaagtgcag cagtttaaat tctgtcggcc ccatctccag
   100141 cggctcttcg cccgccatca ctcggtgaga tgtcgggtcg agacttaatc cctgcatctc
   100201 aatcacctct tccaccgcca ttggcgaaat acggcgcatt accgctttga ttcgcgccac
   100261 cagctccttc ggcgaaaacg gcttggtgat atagtcatcc gcgccggttt caaggccgcg
   100321 cacgcgatct tcttcttccc ctctggcggt caacatcacc actggaatat cccgggtcat
   100381 cgactcgcgc ttgaggtgtt tgatgaactg gataccggag ccgccaggta acatccagtc
   100441 aaggagaatt aaatccggcc agggttcatt cagttgattc acagcactgt cataatcttc
   100501 cgcttcgacc ggctgaaagc cattttgttc gagcacgaag cagaccattt cgcgaattgg
   100561 agcttcatct tctacgacca gaatacgtct cgccatgatt tgccctgttg taataaatag
   100621 gttgcgatca ttaatgcgac gtcattatgc gtcagattta tgacagattt atgaaaagct
   100681 cgtcgcacat atcttcaggt tattgatttc cgtggcgcag aaaaaagcaa atggcacatc
   100741 tgtttgggta taatcgcgcc catgcttttt cgccagggaa ccgttatgcg catccttcac
   100801 acctcagact ggcatctcgg ccagaacttc tacagtaaaa gccgcgaagc tgaacatcag
   100861 gcttttcttg actggctgct ggagacagca caaacccatc aggtggatgc gattattgtt
   100921 gccggtgatg ttttcgatac cggctcgccg cccagttacg cccgcacgtt atacaaccgt
   100981 tttgttgtca atttacagca aactggctgt catctggtgg tactggcagg aaaccatgac
   101041 tcggtcgcca cgctgaatga atcgcgcgat atcatggcgt tcctcaatac taccgtggtc
   101101 gccagcgccg gacatgcgcc gcaaatcttg cctcgtcgcg acgggacgcc aggcgcagtg
   101161 ctgtgcccca ttccgttttt acgtccgcgt gacattatta ccagccaggc ggggcttaac
   101221 ggtattgaaa aacagcagca tttactggca gcgattaccg attattacca acaacactat
   101281 gccgatgcct gcaaactgcg cggcgatcag cctctgccca tcatcgccac gggacattta
   101341 acgaccgtgg gggccagtaa aagtgacgcc gtgcgtgaca tttatattgg cacgctggac
   101401 gcgtttccgg cacaaaactt tccaccagcc gactacatcg cgctcgggca tattcaccgc
   101461 gcacagatta ttggcggcat ggaacatgtt cgctattgcg gctcccccat tccactgagt
   101521 tttgatgaat gcggtaagag taaatatgtc catctggtga cattttcaaa cggcaaatta
   101581 gagagcgtgg aaaacctgaa cgtaccggta acgcaaccca tggcagtgct gaaaggcgat
   101641 ctggcgtcga ttaccgcaca gctggaacag tggcgcgatg tatcgcagga gccacctgtc
   101701 tggctggata tcgaaatcac tactgatgag tatctgcatg atattcagcg caaaatccag
   101761 gcattaaccg aatcattgcc tgttgaagta ttgctggtac gtcggagtcg tgaacagcgc
   101821 gagcgtgtgt tagccagcca acagcgtgaa accctcagcg aactcagcgt cgaagaggtg
   101881 ttcaatcgcc gtctggcact ggaagaactg gatgaatcgc agcagcaacg tctgcagcat
   101941 cttttcacca cgacgttgca taccctcgcc ggagaacacg aagcatgaaa attctcagcc
   102001 tgcgcctgaa aaacctgaac tcattaaaag gcgaatggaa gattgatttc acccgcgagc
   102061 cgttcgccag caacgggctg tttgccatta ccggcccgac cggtgcgggg aaaaccaccc
   102121 tgctggacgc catttgtctg gcgctgtatc acgaaactcc gcgtctctct aacgtttcac
   102181 aatcgcaaaa tgatctcatg acccgcgata ccgctgaatg tctggcggag gtggagtttg
   102241 aagtgaaagg tgaagcgtat cgcgcgttct ggagccagaa tcgggcgcgt aaccaacccg
   102301 acggtaattt gcaggtgcca cgcgtagagc tggcgcgctg cgccgacggc aaaattctcg
   102361 ccgacaaagt gaaagataag ctggaactga cagcgacgtt aaccgggctg gattacgggc
   102421 gcttcacccg ttcgatgctg ctttcgcagg ggcaatttgc tgccttcctg aatgccaaac
   102481 ccaaagaacg cgcggaattg ctcgaggagt taaccggcac tgaaatctac gggcaaatct
   102541 cggcgatggt ttttgagcag cacaaatcgg cccgcacaga gctggagaag ctgcaagcgc
   102601 aggccagcgg cgtcgcgttg ctcacgccgg aacaagtgca atcgctgaca gcgagtttgc
   102661 aggtacttac tgacgaagaa aaacagttac ttaccgcgca gcagcaagaa caacaatcac
   102721 taaactggtt aacgcgtctg gacgaattgc agcaagaagc cagccgccgt cagcaggcct
   102781 tgcaacaggc gttagccgaa gaagaaaaag cgcaacctca actggcggcg ctcagtctgg
   102841 cacaaccggc acgaaatctt cgtccgcact gggaacgcat cgcagaacac agcgcggcgc
   102901 tggcgcatac tcgccagcag attgaagaag taaatactcg cttacagaac acaatggcgc
   102961 ttcgcgcgag cattcgccac cacgcggcga agcagtcagc agaattacag cagcagcaac
   103021 aaagcctgaa tacctggtta caggaacacg accgcttccg tcagtggaac aacgaactgg
   103081 cgggttggcg tgcgcagttc tcccaacaaa ccagcgatcg cgagcatctg cggcaatggc
   103141 agcaacagtt aacccatgct gagcaaaaac ttaatgcgct tgcggcgatc acgttgatgt
   103201 taaccgccga tgaagttgct accgccctgg cgcaacatgc tgagcaacgc ccactgcgtc
   103261 agcgcctggt cgcgctgcat gggcagattg ttccccaaca aaaacgtctg gcgcagttaa
   103321 tggtcactat ccagaatgtc actctggaac agacgcaacg taatgccgca cttaacgaaa
   103381 tgcgccagcg ttataaagaa aagacgcagc aacttgccga tgtgaaaacc atttgcgagc
   103441 aggaagcgcg catcaaaacg ctggaagctc aacgtgcaca gttacaggcg ggtcagcctt
   103501 gtccactttg tggttccacc agccatccgg cggtcgaggc gtatcaggcg ctggagcctg
   103561 gcgttaatca gtctcgatta ctggcgctgg aaaacgaagt taaaaagctc ggcgaagaag
   103621 gtgcggcgct gcgcgggcaa ctggatgcat taacgaagca gcttcagcgc gatgaaaacg
   103681 aagcgcaaag cctccgacaa gatgagcaag cacttactca acaatggcaa gccgtcacgg
   103741 ccagcctcaa tatcaccttg cagccacagg acgatattca accgtggctg gatgcacaag
   103801 atgagcacga acgccagctg cggttactca gccaacgaca tgaattacaa gggcagattg
   103861 ccgcgcataa tcagcaaatt atccagtatc aacagcaaat tgaacaacgc cagcagcagc
   103921 tcttaacggc attggcgggt tatgcactga cattgccaca ggaagatgaa gaagagagct
   103981 ggttggcgac acgtcagcaa gaagcgcaga gctggcagca acgccagaac gaattaaccg
   104041 cgctgcaaaa ccgtattcag cagctgacgc cgattctgga aacgttgccg caaagtgatg
   104101 atctcccgca cagcgaagaa actgtggcgc tggataactg gcggcaggtt catgaacaat
   104161 gtctcgcatt acacagccag cagcagacgt tacagcaaca ggatgttctg gcggcgcaaa
   104221 gtctgcaaaa agcccaggcg cagtttgaca ccgcgctaca ggccagcgtc tttgacgatc
   104281 agcaggcgtt ccttgcggcg ctaatggatg aacaaacact aacgcagctg gaacagctca
   104341 agcagaatct ggaaaaccag cgccgtcagg cgcaaacgct ggtcactcag acagcagaaa
   104401 cgctggcaca gcatcaacaa caccgacctg acgggttggc tctcactgtg acggtggagc
   104461 agattcagca agagttagcg caaactcacc aaaagttgcg tgaaaacacc acgagtcaag
   104521 gcgagattcg ccagcagctg aagcaggatg cagataaccg tcagcaacaa caaaccttac
   104581 tgcagcaaat tgctcaaatg acgcagcagg ttgaggactg gggatatctg aattcgctaa
   104641 taggttccaa agagggcgat aaattccgca agtttgccca ggggctgacg ctggataatt
   104701 tagtccatct cgctaatcag caacttaccc ggctgcacgg gcgctatctg ttacagcgca
   104761 aagccagcga ggcgctggaa gtcgaggttg ttgatacctg gcaggcagat gcggtacgcg
   104821 atacccgtac cctttccggc ggcgaaagtt tcctcgttag tctggcgctg gcgctggcgc
   104881 tttcggatct ggtcagccat aaaacacgta ttgactcgct gttccttgat gaaggttttg
   104941 gcacgctgga tagcgaaacg ctggataccg cccttgatgc gctggatgcc ctgaacgcca
   105001 gtggcaaaac catcggtgtg attagccacg ttgaagcgat gaaagagcgt attcctgtgc
   105061 agatcaaagt gaagaagatc aacggcctgg gctacagcaa actggaaagt acgtttgcag
   105121 tgaaataact attcagcagg ataatgaata cagaggggcg aattatctct tggccttgct
   105181 ggtcgttatc ctgcaagcta tcactttatt ggctacggtg attggtagcc gttctggtag
   105241 ttgtgatggt ggtatgaaaa aagtcatttt atctttggct ctgggcacgt ttggtttggg
   105301 gatggccgaa tttggcatta tgggcgtgct cacggagctg gcgcataacg taggaatttc
   105361 gattcctgct gccgggcata tgatctcgta ttatgcgctg ggggtggtgg tcggtgcgcc
   105421 aataatcgca ctcttttcca gccgctactc actcaaacat atcttgttgt ttctggtggc
   105481 gttgtgcgtc attggcaacg ccatgttcac gctctcttcg tcgtacctga tgcttgccat
   105541 tggtcgcctg gtttccggct ttccgcatgg cgcatttttt ggcgtcggag cgatcgtgtt
   105601 atcaaaaatt atcaaacccg gaaaagtcac cgccgccgtg gcggggatgg tttccgggat
   105661 gacagtcgcc aatttgctgg gcattccgct gggaacgtat ttaagtcagg aatttagctg
   105721 gcgttacacc tttttattga tcgctgtttt taatattgcg gtgatggcat cggtctattt
   105781 ttgggtgccg gatattcgcg acgaggcgaa aggaaagctg cgcgaacaat ttcacttttt
   105841 gcgcagcccg gccccgtggt taattttcgc cgccaccatg tttggcaacg caggtgtgtt
   105901 tgcctggttc agctacgtaa agccatacat gatgtttatt tccggttttt cggaaacggc
   105961 gatgaccttt attatgatgt tagttgggct agggatggtg ctgggaaata tgctaagtgg
   106021 caggatttca ggacgttatt caccactgcg cattgcagca gtgactgact ttataattgt
   106081 actggcactg ctgatgctct ttttctgcgg cggcatgaaa acaacgtcgc ttatttttgc
   106141 ttttatttgt tgcgcgggat tatttgccct ttcagcacct ctgcaaatat tgttactgca
   106201 aaatgccaaa ggcggagagt tattaggtgc cgcaggtggg caaatagcgt ttaacctcgg
   106261 tagcgccgtc ggcgcatatt gcggaggtat gatgctgacg ctggggctgg catataatta
   106321 cgtggcgctg cctgccgccc tgctttcgtt tgctgcgatg tcatcgttgc tgctgtatgg
   106381 tcgctataag cgccagcaag cggcggatag tccggtgctg gcgaaaccac tggggtagat
   106441 tactgtcacg gtgtgcgttg cttattgccg gatgcggcgt gaacgcctta tccgccctac
   106501 gcggttctgg cacattttgt aggcctgata agacgcggca agcgtcgcat caggcatgat
   106561 gcgccaattg cctgggtttt ttactcttgt ggccataacc acgcagcgcc gcgtacgccg
   106621 ctggaatcac cgtgcttcgc cttacgcacc ggcgtttcac attcgccgcc gaagacaaat
   106681 tgtttaatca actgcggaac cgtttgatat aaacggtcta cattgctcat cccgcccccc
   106741 aggacaatca catccggatc gagaatattc acgacatgtg ccagcgattt tgccagccgc
   106801 agctcgtagc gacgcaatgc cagttccgct accggatcgc tttcttcaac caggcggata
   106861 atttcactgc ctttcagcgc atgtccgctc aaacgacgat aatccgtcgc gaatcccgtg
   106921 cccgaaataa aggtttcaat acaaccttgt ttaccgcaat aacaagggac ttcctcgcga
   106981 taacgcagtt cgtcttcgtc catccacggt agcggattgt gtccccactc acctgccgtg
   107041 ccattgccgc cgatatgcgc ccgcccattg aatgccacgc ccgcgccgca tcccgtgccg
   107101 ataatcacgg caaataccgt ctgcgctccc gctgccgcgc catctactgc ttctgaaacc
   107161 gccagacagt tggcgtcatt tgccagccgc acttcccgct gcaacctcgc gcttaagtct
   107221 ttatcgaatg gctgaccgtt gagccaggtt gaattggcat tcttcaccac accggtgtaa
   107281 ggcgaaattg agccaggaat gcccatacct accgttccgc gctgccccgt cgcctgctcc
   107341 gccatatcaa ccaacgtggc gatcgtttca atagtctgcc ggtaatcatc acgcggcgtg
   107401 ggcagacgat ggcggtacaa ctgctcccct gcatcgccca gtgcaatcac ttcagttttg
   107461 gtgccgccta aatcgatacc tatacgcacg gtactctcct tatttttttc aatatcaata
   107521 gcgtagagac ggacaaccgg attggcaatg caaggccgcc gacaattcgt tatcatgccc
   107581 gctaaattta acgacaaggc cgtggaaatt atcatgctgt ggttcaaaaa tttaatggtt
   107641 taccgtctta gccgcgagat ttcgctgcgt gcagaagaga tggaaaaaca gctagcctcg
   107701 atggcattta ccccatgcgg cagccaggac atggcgaaga tgggctgggt tcctccgatg
   107761 ggatcgcaca gcgatgcgtt aacgcacgtt gccaatggtc aaattgttat ctgcgcgcgc
   107821 aaagaagaaa aaatcctacc gtctccggtg attaaacagg cgctggaagc gaaaatcgcc
   107881 aaactggaag cggaacaggc gcgtaagctg aagaaaaccg aaaaagattc gctgaaagac
   107941 gaagtgctgc actctctgtt gccgcgtgct ttcagccgtt ttagccagac aatgatgtgg
   108001 atcgacacgg ttaacggttt gattatggtg gactgcgcca gtgccaaaaa agcggaagat
   108061 acgctggcac tgctgcgtaa aagcctgggg tcgttaccgg ttgtaccatt gagcatggca
   108121 aacccgattg aactgacgct gaccgaatgg gtacgttccg gtagtgcggc acagggcttc
   108181 cagctgcttg atgaagccga gctgaaatcg ttgctggaag atggcggagt gatccgcgcg
   108241 aagaaacaag atctgaccag cgaagagatc accaatcaca ttgaagccgg aaaagtggtg
   108301 actaaactgg cgctcgactg gcagcagcgc attcagtttg tgatgtgcga cgatggttcg
   108361 ctcaagcgtc tgaagttctg cgacgagctg cgcgatcaaa acgaagatat cgaccgtgaa
   108421 gatttcgccc agcgttttga tgccgatttc atcctgatga ctggtgaact ggcagcgtta
   108481 attcaaaacc tgattgaagg attaggtggc gaagcacaac gttaattgct gattttcctt
   108541 taatgccgga tgcgacgcct gccgcgtctt atccggcgta cgaagccaca ccaggcatat
   108601 aattattcgc tacggcgggc aataattttt tagcgcagca atattatgcg ttttacgccg
   108661 taactgatct aatcgcctca tccctattcg cctgtcagca gcacgcagaa tattcactaa
   108721 cccccattta tcgctggtaa attgttttgt taacaactcc gcttttgggg tgtcaatata
   108781 ttcacgcaat aaatcagaaa ttgaacaaat atccgtcagg taaggatacc agcaagtttc
   108841 accgtgaaaa ttacgcactc caccgttgcc tgcaataaag tctttcgggt aatcccagat
   108901 cacgttttta tccaacgtga tccaataacg cggtaaatcg gtgcttccac cattttggct
   108961 gcgcatggga taacgggtac agtgaatttg caggttgatc gttggcgtca gtaagtcata
   109021 gatctcccgc tgtaacttgc tccaggggcg ttgtgtcatt gtttttcctc aagccggtga
   109081 cgcgcttcat tgcgaatttt ccgcgttgcc ccggttttca ctaaatcccg taactgcgaa
   109141 agatcgggtt tatcgacaaa taaaagtaat gatggatcct cttttagcca caccgttttc
   109201 acatcggcag ccagctgtgg attattaata gccagcgacc gatcctgaga ctcagtcaat
   109261 gtcgttaaca acgacgccgg ggtatgtgcg ttgcgtaatg ccgtttggcg caaatgatca
   109321 ttgcgacttt gcagcaatgc acgccaccat tcatcatctg atttatcttt tatcgtcgcc
   109381 agttccgcaa aaatatcgct atcctcatgg ttccagcggt gtaatcctct ttcactttgt
   109441 ctacgggtgc tatttttcat actgacaaca gccagcgccc agtcagagtt gaagcgatac
   109501 tgttgatcga gtttgttgat aattccggaa ggtaattctt tattatcaaa taattggtta
   109561 cgcattcgat agcggtattc agcgggtaaa tcaaccagta aatcaatggc attatttaac
   109621 atctcttcgt caacaacacc attatcaatc agcgccagct gtacttcgta ggcttgctct
   109681 tgatcttctt gccgtttgct catcattaaa tttaatgtgc gttgccataa ctgtttctta
   109741 tattccagcg gcaataattt atcgcgtgcc agctcatccc agacggtggg aacatcatgc
   109801 tgagtcaata gataactaat cacgtccttc gatgtcagat aacgcgcacc ttcgcgcaaa
   109861 ttgtgcacat cttcttttgc cagagacagt tgacgcatct ccggcaacgc tatcaataac
   109921 acctttacga tatttttatt ttctttatta tccagataca tttgttctgc gatacgttca
   109981 atatcctctg tacgcagggt tgctgacagt tgggtaattt ttaattcagt aagcgtccgg
   110041 gctaatgctt cacgaacttt cagtgattta tctttactca acatcactgc ttgttgtgcg
   110101 gttaaatctg ctccggtaat tctttcacgt aactccgggt cttcgttatt tatcaactca
   110161 tcggccactg cagtcggaac cagccaggcg taatcgtaat ggctgagtaa tgtcttgcgt
   110221 acactgagaa aggggctatg acttaacgac gcaacaatat ctggagaaag attatcctgt
   110281 ctggcctgct ccagctgtac gctctcgacg ggtacagaca ttaatttttt attcacatcg
   110341 gcggaaatat tactgttttt aaccagccct ctggcaacag cctcatcttt aattaattca
   110401 tcggtaagcc aatgcatctg ttgagcgggc caggactgcg ccatcgattc acgcgtctgg
   110461 tcatcggggt gctgtaacag ttgcagcata acggcgggtg ataaactctc tgtatgagtc
   110521 agaaaacgtg ccaccagtgg atcttgatgc tttattttat tcgctgggaa attatcgagt
   110581 ttccccgttt caatccattt taatttctca ttaatttcag gagcggcttt atctcgaaaa
   110641 agcggtaaac cctgaacgat gttgtctgcg acgctatcat agtcaggata cgcattggaa
   110701 atgttatcgg tttcgatttc agtgtgatca taaagatcgg cagtcaccag tgaataggct
   110761 ttttgaatat tctcatccca gttatatcct tcaccacttc catcttcatt gatataaaat
   110821 tcagctttaa tgacttcaaa ttgcgttgct ttatcggaaa caataatgcc tcgcgcacta
   110881 attcccttca tcacttcaaa gttatgatca ttgtaatcag cataaactaa acctttcgct
   110941 gtcatctgtc ctttgacata cgcgaaatca tgattaataa agttatcgac aataacatta
   111001 cctaatacaa tcagatgacc attacctgac agataatcat cataatcgcc ggaaatcgtt
   111061 aaattaccgt tcaccaccag tttattttta ttgctgaagt tagcgggtaa tgataggtca
   111121 ccattatgat aataccagcg gatattatct tttagcatat aagcagcttc catactatcg
   111181 tcggttgccg ctttaaccac gttgcgctca tctgccgtca taagatgaaa gtcagaggtg
   111241 ataaggtcct cgctactgat ccactctgtg gcacatgcat tgccagtcat aaaaaataag
   111301 accatacaga tgaccaatat attaattcta ttcataatct tcaatgatat ttatatcaac
   111361 cttgatgaat tatgaatata tcggaaattg aaaaaatcgc agcaaaggaa atattattat
   111421 ggggttatga gaggacattt cagaaaaatg ccctcccgtt caagcggaag gcgaggaatt
   111481 acagatagcg gcacagataa gaggtgggtt cggcaacttg cagatgaaac tcactgtgac
   111541 cgggaacatt aaacaccgaa ccggcttcat acacttgcca gtcggtcgcg tcaggcagta
   111601 acacattcag cgcgccactg attaccgtca tctcttccgg ctcagcggtg ctgaaggtgt
   111661 attcgccttc aaccataaca cccacgctgg cgcgaccagt gctgctgctg gaaaagccga
   111721 tcgatttcac tttgccggaa aagtactcat tactttgaag cataaactgg ccctgtctgg
   111781 tagttgaata tctacttcac tataggggcc agatgcgctg gctgtcacgc aaaattacac
   111841 cagtaattcc gcagccagcc gtgcaatcaa tacgttagag agcaagacag gaacatcgag
   111901 ctgtttttgc agtaaatcgc gatgacgctg gttaaatccc aaacaatcca gcatgatgac
   111961 atctgcgcct ttcgccagta attctttccc ggcatcaatg attttttgtt ctgaatcatg
   112021 aatggggtta cccaatgaaa atacaggcgg tttctgcaaa atttgccatt tttgcgcctg
   112081 aacggtcagc aactcctcaa ccggtacgat aacccccacc tgatgatctt caacaataga
   112141 ggaaaccagc ggtggcaata tccgcgacgg ctcaagaaag atcgtattac gcgcagtcat
   112201 actactaatg tttgccgtac tcattaatat aatgacgtcg tagccctgat tatcgagcac
   112261 ttcaaccaca ccttgcaggt cacgctctac tttgcgacgc gaaacatggg ccagatggtt
   112321 gtcatttaat aatgtgagaa ttgtgtcttc gcctgcttct ggcgcgtact ccgccatcac
   112381 ttcttcgcgg cttaacttcc ccagcaggct atgatgggaa atattatctt cgtcaatgta
   112441 ttctgtcagg agcggcaaaa cttcctgcat aggtacaatg ccgatggtta gaatcgccaa
   112501 cgacgcactc attgttcttc caccttactt ttatttcgtc gttaattacc gggcaagtgt
   112561 taagcaccac tgctgacact tacttgtgct acaaatgaca aaaagcgtag cagcagacgt
   112621 gcacggcata atgtaaagat tccaaatgat tccagtaat
//
LOCUS       DABSYT020000004       104484 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.004, whole genome shotgun sequence.
ACCESSION   DABSYT020000004 DABSYT020000000
VERSION     DABSYT020000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 104484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 104484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.004"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..182
                     /locus_tag="ID725_RS01925"
                     /old_locus_tag="pgaptmp_000385"
     CDS             <1..182
                     /locus_tag="ID725_RS01925"
                     /old_locus_tag="pgaptmp_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGV7849067.1"
                     /translation="DKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEPASK
                     VTHWMPLPEPPQEVK"
     gene            270..512
                     /locus_tag="ID725_RS01930"
                     /old_locus_tag="pgaptmp_000386"
     CDS             270..512
                     /locus_tag="ID725_RS01930"
                     /old_locus_tag="pgaptmp_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGV7849068.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            516..650
                     /locus_tag="ID725_RS01935"
                     /old_locus_tag="pgaptmp_000387"
     CDS             516..650
                     /locus_tag="ID725_RS01935"
                     /old_locus_tag="pgaptmp_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849069.1"
                     /translation="MHTAFEFWVRKTFGNRYDLNRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            669..923
                     /gene="xisR"
                     /locus_tag="ID725_RS01940"
                     /old_locus_tag="pgaptmp_000388"
     CDS             669..923
                     /gene="xisR"
                     /locus_tag="ID725_RS01940"
                     /old_locus_tag="pgaptmp_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGV7849070.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            957..2243
                     /locus_tag="ID725_RS01945"
                     /old_locus_tag="pgaptmp_000389"
     CDS             957..2243
                     /locus_tag="ID725_RS01945"
                     /old_locus_tag="pgaptmp_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7849071.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            2264..2965
                     /gene="mlrA"
                     /locus_tag="ID725_RS01950"
                     /old_locus_tag="pgaptmp_000390"
     CDS             2264..2965
                     /gene="mlrA"
                     /locus_tag="ID725_RS01950"
                     /old_locus_tag="pgaptmp_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGV7849072.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            3025..3132
                     /gene="yohO"
                     /locus_tag="ID725_RS01955"
                     /old_locus_tag="pgaptmp_000391"
     CDS             3025..3132
                     /gene="yohO"
                     /locus_tag="ID725_RS01955"
                     /old_locus_tag="pgaptmp_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGV7849073.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="ID725_RS01960"
                     /old_locus_tag="pgaptmp_000392"
     CDS             complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="ID725_RS01960"
                     /old_locus_tag="pgaptmp_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGV7849074.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="ID725_RS01965"
                     /old_locus_tag="pgaptmp_000393"
     CDS             complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="ID725_RS01965"
                     /old_locus_tag="pgaptmp_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGV7849075.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="ID725_RS01970"
                     /old_locus_tag="pgaptmp_000394"
     CDS             complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="ID725_RS01970"
                     /old_locus_tag="pgaptmp_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGV7849076.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="ID725_RS01975"
                     /old_locus_tag="pgaptmp_000395"
     CDS             complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="ID725_RS01975"
                     /old_locus_tag="pgaptmp_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGV7849077.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="ID725_RS01980"
                     /old_locus_tag="pgaptmp_000396"
     CDS             complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="ID725_RS01980"
                     /old_locus_tag="pgaptmp_000396"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGV7849078.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9594..11309
                     /gene="dld"
                     /locus_tag="ID725_RS01985"
                     /old_locus_tag="pgaptmp_000397"
     CDS             9594..11309
                     /gene="dld"
                     /locus_tag="ID725_RS01985"
                     /old_locus_tag="pgaptmp_000397"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGV7849079.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="ID725_RS01990"
                     /old_locus_tag="pgaptmp_000398"
     CDS             complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="ID725_RS01990"
                     /old_locus_tag="pgaptmp_000398"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGV7849080.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="ID725_RS01995"
                     /old_locus_tag="pgaptmp_000399"
     CDS             complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="ID725_RS01995"
                     /old_locus_tag="pgaptmp_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGV7849081.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGDMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            13210..13788
                     /gene="yohD"
                     /locus_tag="ID725_RS02000"
                     /old_locus_tag="pgaptmp_000400"
     CDS             13210..13788
                     /gene="yohD"
                     /locus_tag="ID725_RS02000"
                     /old_locus_tag="pgaptmp_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGV7849082.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="ID725_RS02005"
                     /old_locus_tag="pgaptmp_000401"
     CDS             complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="ID725_RS02005"
                     /old_locus_tag="pgaptmp_000401"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGV7849083.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="ID725_RS02010"
                     /old_locus_tag="pgaptmp_000402"
     CDS             complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="ID725_RS02010"
                     /old_locus_tag="pgaptmp_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGV7849084.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            16392..16475
                     /gene="yohP"
                     /locus_tag="ID725_RS02015"
                     /old_locus_tag="pgaptmp_000403"
     CDS             16392..16475
                     /gene="yohP"
                     /locus_tag="ID725_RS02015"
                     /old_locus_tag="pgaptmp_000403"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGV7849085.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(16863..17813)
                     /gene="dusC"
                     /locus_tag="ID725_RS02020"
                     /old_locus_tag="pgaptmp_000404"
     CDS             complement(16863..17813)
                     /gene="dusC"
                     /locus_tag="ID725_RS02020"
                     /old_locus_tag="pgaptmp_000404"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGV7849086.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(17972..19165)
                     /locus_tag="ID725_RS02025"
                     /old_locus_tag="pgaptmp_000405"
     CDS             complement(17972..19165)
                     /locus_tag="ID725_RS02025"
                     /old_locus_tag="pgaptmp_000405"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HGV7849087.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(19180..19824)
                     /gene="maiA"
                     /locus_tag="ID725_RS02030"
                     /old_locus_tag="pgaptmp_000406"
     CDS             complement(19180..19824)
                     /gene="maiA"
                     /locus_tag="ID725_RS02030"
                     /old_locus_tag="pgaptmp_000406"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HGV7849088.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(19833..20534)
                     /locus_tag="ID725_RS02035"
                     /old_locus_tag="pgaptmp_000407"
     CDS             complement(19833..20534)
                     /locus_tag="ID725_RS02035"
                     /old_locus_tag="pgaptmp_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGV7849089.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(20549..21577)
                     /gene="gtdA"
                     /locus_tag="ID725_RS02040"
                     /old_locus_tag="pgaptmp_000408"
     CDS             complement(20549..21577)
                     /gene="gtdA"
                     /locus_tag="ID725_RS02040"
                     /old_locus_tag="pgaptmp_000408"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HGV7849090.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(21589..22947)
                     /locus_tag="ID725_RS02045"
                     /old_locus_tag="pgaptmp_000409"
     CDS             complement(21589..22947)
                     /locus_tag="ID725_RS02045"
                     /old_locus_tag="pgaptmp_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7849091.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASRVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            23074..23982
                     /locus_tag="ID725_RS02050"
                     /old_locus_tag="pgaptmp_000410"
     CDS             23074..23982
                     /locus_tag="ID725_RS02050"
                     /old_locus_tag="pgaptmp_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGV7849092.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            24113..24511
                     /gene="yohJ"
                     /locus_tag="ID725_RS02055"
                     /old_locus_tag="pgaptmp_000411"
     CDS             24113..24511
                     /gene="yohJ"
                     /locus_tag="ID725_RS02055"
                     /old_locus_tag="pgaptmp_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGV7849093.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            24508..25203
                     /gene="yohK"
                     /locus_tag="ID725_RS02060"
                     /old_locus_tag="pgaptmp_000412"
     CDS             24508..25203
                     /gene="yohK"
                     /locus_tag="ID725_RS02060"
                     /old_locus_tag="pgaptmp_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGV7849094.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            25333..26217
                     /gene="cdd"
                     /locus_tag="ID725_RS02065"
                     /old_locus_tag="pgaptmp_000413"
     CDS             25333..26217
                     /gene="cdd"
                     /locus_tag="ID725_RS02065"
                     /old_locus_tag="pgaptmp_000413"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGV7849095.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            26367..27086
                     /gene="sanA"
                     /locus_tag="ID725_RS02070"
                     /old_locus_tag="pgaptmp_000414"
     CDS             26367..27086
                     /gene="sanA"
                     /locus_tag="ID725_RS02070"
                     /old_locus_tag="pgaptmp_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGV7849096.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            27089..27328
                     /gene="yeiS"
                     /locus_tag="ID725_RS02075"
                     /old_locus_tag="pgaptmp_000415"
     CDS             27089..27328
                     /gene="yeiS"
                     /locus_tag="ID725_RS02075"
                     /old_locus_tag="pgaptmp_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGV7849097.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            27522..28760
                     /gene="preT"
                     /locus_tag="ID725_RS02080"
                     /old_locus_tag="pgaptmp_000416"
     CDS             27522..28760
                     /gene="preT"
                     /locus_tag="ID725_RS02080"
                     /old_locus_tag="pgaptmp_000416"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGV7849098.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            28754..29989
                     /gene="preA"
                     /locus_tag="ID725_RS02085"
                     /old_locus_tag="pgaptmp_000417"
     CDS             28754..29989
                     /gene="preA"
                     /locus_tag="ID725_RS02085"
                     /old_locus_tag="pgaptmp_000417"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGV7849099.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(30232..31242)
                     /gene="mglC"
                     /locus_tag="ID725_RS02090"
                     /old_locus_tag="pgaptmp_000418"
     CDS             complement(30232..31242)
                     /gene="mglC"
                     /locus_tag="ID725_RS02090"
                     /old_locus_tag="pgaptmp_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGV7849100.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(31258..32778)
                     /gene="mglA"
                     /locus_tag="ID725_RS02095"
                     /old_locus_tag="pgaptmp_000419"
     CDS             complement(31258..32778)
                     /gene="mglA"
                     /locus_tag="ID725_RS02095"
                     /old_locus_tag="pgaptmp_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGV7849101.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKRDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(32839..33837)
                     /gene="mglB"
                     /locus_tag="ID725_RS02100"
                     /old_locus_tag="pgaptmp_000420"
     CDS             complement(32839..33837)
                     /gene="mglB"
                     /locus_tag="ID725_RS02100"
                     /old_locus_tag="pgaptmp_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGV7849102.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(34116..35156)
                     /gene="galS"
                     /locus_tag="ID725_RS02105"
                     /old_locus_tag="pgaptmp_000421"
     CDS             complement(34116..35156)
                     /gene="galS"
                     /locus_tag="ID725_RS02105"
                     /old_locus_tag="pgaptmp_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGV7849103.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(35298..36455)
                     /gene="yeiB"
                     /locus_tag="ID725_RS02110"
                     /old_locus_tag="pgaptmp_000422"
     CDS             complement(35298..36455)
                     /gene="yeiB"
                     /locus_tag="ID725_RS02110"
                     /old_locus_tag="pgaptmp_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGV7849104.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(36472..37140)
                     /gene="folE"
                     /locus_tag="ID725_RS02115"
                     /old_locus_tag="pgaptmp_000423"
     CDS             complement(36472..37140)
                     /gene="folE"
                     /locus_tag="ID725_RS02115"
                     /old_locus_tag="pgaptmp_000423"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGV7849105.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            37398..38234
                     /gene="yeiG"
                     /locus_tag="ID725_RS02120"
                     /old_locus_tag="pgaptmp_000424"
     CDS             37398..38234
                     /gene="yeiG"
                     /locus_tag="ID725_RS02120"
                     /old_locus_tag="pgaptmp_000424"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGV7849106.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(38266..40245)
                     /gene="cirA"
                     /locus_tag="ID725_RS02125"
                     /old_locus_tag="pgaptmp_000425"
     CDS             complement(38266..40245)
                     /gene="cirA"
                     /locus_tag="ID725_RS02125"
                     /old_locus_tag="pgaptmp_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HGV7849107.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(40537..42006)
                     /gene="lysP"
                     /locus_tag="ID725_RS02130"
                     /old_locus_tag="pgaptmp_000426"
     CDS             complement(40537..42006)
                     /gene="lysP"
                     /locus_tag="ID725_RS02130"
                     /old_locus_tag="pgaptmp_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGV7849108.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(42211..43092)
                     /gene="yieE"
                     /locus_tag="ID725_RS02135"
                     /old_locus_tag="pgaptmp_000427"
     CDS             complement(42211..43092)
                     /gene="yieE"
                     /locus_tag="ID725_RS02135"
                     /old_locus_tag="pgaptmp_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGV7849109.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            43191..44240
                     /gene="yeiH"
                     /locus_tag="ID725_RS02140"
                     /old_locus_tag="pgaptmp_000428"
     CDS             43191..44240
                     /gene="yeiH"
                     /locus_tag="ID725_RS02140"
                     /old_locus_tag="pgaptmp_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGV7849110.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            44314..45171
                     /gene="nfo"
                     /locus_tag="ID725_RS02145"
                     /old_locus_tag="pgaptmp_000429"
     CDS             44314..45171
                     /gene="nfo"
                     /locus_tag="ID725_RS02145"
                     /old_locus_tag="pgaptmp_000429"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGV7849111.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            45174..46262
                     /gene="yeiI"
                     /locus_tag="ID725_RS02150"
                     /old_locus_tag="pgaptmp_000430"
     CDS             45174..46262
                     /gene="yeiI"
                     /locus_tag="ID725_RS02150"
                     /old_locus_tag="pgaptmp_000430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGV7849112.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(46318..47568)
                     /gene="nupX"
                     /locus_tag="ID725_RS02155"
                     /old_locus_tag="pgaptmp_000431"
     CDS             complement(46318..47568)
                     /gene="nupX"
                     /locus_tag="ID725_RS02155"
                     /old_locus_tag="pgaptmp_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGV7849113.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(47668..48609)
                     /gene="rihB"
                     /locus_tag="ID725_RS02160"
                     /old_locus_tag="pgaptmp_000432"
     CDS             complement(47668..48609)
                     /gene="rihB"
                     /locus_tag="ID725_RS02160"
                     /old_locus_tag="pgaptmp_000432"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGV7849114.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            48739..49437
                     /gene="yeiL"
                     /locus_tag="ID725_RS02165"
                     /old_locus_tag="pgaptmp_000433"
     CDS             48739..49437
                     /gene="yeiL"
                     /locus_tag="ID725_RS02165"
                     /old_locus_tag="pgaptmp_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGV7849115.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(49508..50758)
                     /locus_tag="ID725_RS02170"
                     /old_locus_tag="pgaptmp_000434"
     CDS             complement(49508..50758)
                     /locus_tag="ID725_RS02170"
                     /old_locus_tag="pgaptmp_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGV7849116.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(50852..51790)
                     /gene="psuG"
                     /locus_tag="ID725_RS02175"
                     /old_locus_tag="pgaptmp_000435"
     CDS             complement(50852..51790)
                     /gene="psuG"
                     /locus_tag="ID725_RS02175"
                     /old_locus_tag="pgaptmp_000435"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGV7849117.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(51778..52719)
                     /gene="psuK"
                     /locus_tag="ID725_RS02180"
                     /old_locus_tag="pgaptmp_000436"
     CDS             complement(51778..52719)
                     /gene="psuK"
                     /locus_tag="ID725_RS02180"
                     /old_locus_tag="pgaptmp_000436"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGV7849118.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(53143..54834)
                     /gene="fruA"
                     /locus_tag="ID725_RS02185"
                     /old_locus_tag="pgaptmp_000437"
     CDS             complement(53143..54834)
                     /gene="fruA"
                     /locus_tag="ID725_RS02185"
                     /old_locus_tag="pgaptmp_000437"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGV7849119.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(54851..55789)
                     /gene="fruK"
                     /locus_tag="ID725_RS02190"
                     /old_locus_tag="pgaptmp_000438"
     CDS             complement(54851..55789)
                     /gene="fruK"
                     /locus_tag="ID725_RS02190"
                     /old_locus_tag="pgaptmp_000438"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGV7849120.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(55789..56919)
                     /gene="fruB"
                     /locus_tag="ID725_RS02195"
                     /old_locus_tag="pgaptmp_000439"
     CDS             complement(55789..56919)
                     /gene="fruB"
                     /locus_tag="ID725_RS02195"
                     /old_locus_tag="pgaptmp_000439"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGV7849121.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            57287..58468
                     /gene="setB"
                     /locus_tag="ID725_RS02200"
                     /old_locus_tag="pgaptmp_000440"
     CDS             57287..58468
                     /gene="setB"
                     /locus_tag="ID725_RS02200"
                     /old_locus_tag="pgaptmp_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGV7849122.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(58465..58719)
                     /gene="yeiW"
                     /locus_tag="ID725_RS02205"
                     /old_locus_tag="pgaptmp_000441"
     CDS             complement(58465..58719)
                     /gene="yeiW"
                     /locus_tag="ID725_RS02205"
                     /old_locus_tag="pgaptmp_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGV7849123.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            58874..59446
                     /gene="yeiP"
                     /locus_tag="ID725_RS02210"
                     /old_locus_tag="pgaptmp_000442"
     CDS             58874..59446
                     /gene="yeiP"
                     /locus_tag="ID725_RS02210"
                     /old_locus_tag="pgaptmp_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGV7849124.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            59669..61135
                     /gene="yeiQ"
                     /locus_tag="ID725_RS02215"
                     /old_locus_tag="pgaptmp_000443"
     CDS             59669..61135
                     /gene="yeiQ"
                     /locus_tag="ID725_RS02215"
                     /old_locus_tag="pgaptmp_000443"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGV7849125.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            61253..62239
                     /gene="yeiR"
                     /locus_tag="ID725_RS02220"
                     /old_locus_tag="pgaptmp_000444"
     CDS             61253..62239
                     /gene="yeiR"
                     /locus_tag="ID725_RS02220"
                     /old_locus_tag="pgaptmp_000444"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGV7849126.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            62278..62991
                     /gene="lpxT"
                     /locus_tag="ID725_RS02225"
                     /old_locus_tag="pgaptmp_000445"
     CDS             62278..62991
                     /gene="lpxT"
                     /locus_tag="ID725_RS02225"
                     /old_locus_tag="pgaptmp_000445"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGV7849127.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            63404..63970
                     /gene="mepS"
                     /locus_tag="ID725_RS02230"
                     /old_locus_tag="pgaptmp_000446"
     CDS             63404..63970
                     /gene="mepS"
                     /locus_tag="ID725_RS02230"
                     /old_locus_tag="pgaptmp_000446"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGV7849128.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            64151..65707
                     /gene="pdeN"
                     /locus_tag="ID725_RS02235"
                     /old_locus_tag="pgaptmp_000447"
     CDS             64151..65707
                     /gene="pdeN"
                     /locus_tag="ID725_RS02235"
                     /old_locus_tag="pgaptmp_000447"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGV7849129.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            65789..67603
                     /gene="yejA"
                     /locus_tag="ID725_RS02240"
                     /old_locus_tag="pgaptmp_000448"
     CDS             65789..67603
                     /gene="yejA"
                     /locus_tag="ID725_RS02240"
                     /old_locus_tag="pgaptmp_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGV7849130.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            67604..68698
                     /gene="yejB"
                     /locus_tag="ID725_RS02245"
                     /old_locus_tag="pgaptmp_000449"
     CDS             67604..68698
                     /gene="yejB"
                     /locus_tag="ID725_RS02245"
                     /old_locus_tag="pgaptmp_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGV7849131.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            68698..69723
                     /gene="yejE"
                     /locus_tag="ID725_RS02250"
                     /old_locus_tag="pgaptmp_000450"
     CDS             68698..69723
                     /gene="yejE"
                     /locus_tag="ID725_RS02250"
                     /old_locus_tag="pgaptmp_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7849132.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            69725..71314
                     /gene="yejF"
                     /locus_tag="ID725_RS02255"
                     /old_locus_tag="pgaptmp_000451"
     CDS             69725..71314
                     /gene="yejF"
                     /locus_tag="ID725_RS02255"
                     /old_locus_tag="pgaptmp_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGV7849133.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(71318..71662)
                     /gene="yejG"
                     /locus_tag="ID725_RS02260"
                     /old_locus_tag="pgaptmp_000452"
     CDS             complement(71318..71662)
                     /gene="yejG"
                     /locus_tag="ID725_RS02260"
                     /old_locus_tag="pgaptmp_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGV7849134.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(71995..73185)
                     /gene="bcr"
                     /locus_tag="ID725_RS02265"
                     /old_locus_tag="pgaptmp_000453"
     CDS             complement(71995..73185)
                     /gene="bcr"
                     /locus_tag="ID725_RS02265"
                     /old_locus_tag="pgaptmp_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGV7849135.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(73213..73908)
                     /gene="rsuA"
                     /locus_tag="ID725_RS02270"
                     /old_locus_tag="pgaptmp_000454"
     CDS             complement(73213..73908)
                     /gene="rsuA"
                     /locus_tag="ID725_RS02270"
                     /old_locus_tag="pgaptmp_000454"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGV7849136.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            74057..75817
                     /gene="radD"
                     /locus_tag="ID725_RS02275"
                     /old_locus_tag="pgaptmp_000455"
     CDS             74057..75817
                     /gene="radD"
                     /locus_tag="ID725_RS02275"
                     /old_locus_tag="pgaptmp_000455"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGV7849137.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            75942..76226
                     /gene="rplY"
                     /locus_tag="ID725_RS02280"
                     /old_locus_tag="pgaptmp_000456"
     CDS             75942..76226
                     /gene="rplY"
                     /locus_tag="ID725_RS02280"
                     /old_locus_tag="pgaptmp_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGV7849138.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(76365..77372)
                     /gene="yejK"
                     /locus_tag="ID725_RS02285"
                     /old_locus_tag="pgaptmp_000457"
     CDS             complement(76365..77372)
                     /gene="yejK"
                     /locus_tag="ID725_RS02285"
                     /old_locus_tag="pgaptmp_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGV7849139.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            77554..77781
                     /gene="yejL"
                     /locus_tag="ID725_RS02290"
                     /old_locus_tag="pgaptmp_000458"
     CDS             77554..77781
                     /gene="yejL"
                     /locus_tag="ID725_RS02290"
                     /old_locus_tag="pgaptmp_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGV7849140.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            77801..79561
                     /gene="yejM"
                     /locus_tag="ID725_RS02295"
                     /old_locus_tag="pgaptmp_000459"
     CDS             77801..79561
                     /gene="yejM"
                     /locus_tag="ID725_RS02295"
                     /old_locus_tag="pgaptmp_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGV7849141.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            79636..79712
                     /locus_tag="ID725_RS02300"
                     /old_locus_tag="pgaptmp_000460"
     tRNA            79636..79712
                     /locus_tag="ID725_RS02300"
                     /old_locus_tag="pgaptmp_000460"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:79670..79672,aa:Pro,seq:ggg)
     gene            complement(79813..82404)
                     /gene="yejO"
                     /locus_tag="ID725_RS02305"
                     /old_locus_tag="pgaptmp_000461"
     CDS             complement(79813..82404)
                     /gene="yejO"
                     /locus_tag="ID725_RS02305"
                     /old_locus_tag="pgaptmp_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HGV7849142.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            82724..83371
                     /gene="narP"
                     /locus_tag="ID725_RS02310"
                     /old_locus_tag="pgaptmp_000462"
     CDS             82724..83371
                     /gene="narP"
                     /locus_tag="ID725_RS02310"
                     /old_locus_tag="pgaptmp_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGV7849143.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(83406..84458)
                     /gene="ccmH"
                     /locus_tag="ID725_RS02315"
                     /old_locus_tag="pgaptmp_000463"
     CDS             complement(83406..84458)
                     /gene="ccmH"
                     /locus_tag="ID725_RS02315"
                     /old_locus_tag="pgaptmp_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGV7849144.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(84455..85012)
                     /gene="dsbE"
                     /locus_tag="ID725_RS02320"
                     /old_locus_tag="pgaptmp_000464"
     CDS             complement(84455..85012)
                     /gene="dsbE"
                     /locus_tag="ID725_RS02320"
                     /old_locus_tag="pgaptmp_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGV7849145.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(85009..86952)
                     /gene="ccmF"
                     /locus_tag="ID725_RS02325"
                     /old_locus_tag="pgaptmp_000465"
     CDS             complement(85009..86952)
                     /gene="ccmF"
                     /locus_tag="ID725_RS02325"
                     /old_locus_tag="pgaptmp_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGV7849146.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(86949..87428)
                     /gene="ccmE"
                     /locus_tag="ID725_RS02330"
                     /old_locus_tag="pgaptmp_000466"
     CDS             complement(86949..87428)
                     /gene="ccmE"
                     /locus_tag="ID725_RS02330"
                     /old_locus_tag="pgaptmp_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGV7849147.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(87425..87634)
                     /gene="ccmD"
                     /locus_tag="ID725_RS02335"
                     /old_locus_tag="pgaptmp_000467"
     CDS             complement(87425..87634)
                     /gene="ccmD"
                     /locus_tag="ID725_RS02335"
                     /old_locus_tag="pgaptmp_000467"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGV7849148.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(87631..88368)
                     /gene="ccmC"
                     /locus_tag="ID725_RS02340"
                     /old_locus_tag="pgaptmp_000468"
     CDS             complement(87631..88368)
                     /gene="ccmC"
                     /locus_tag="ID725_RS02340"
                     /old_locus_tag="pgaptmp_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGV7849149.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(88410..89072)
                     /gene="ccmB"
                     /locus_tag="ID725_RS02345"
                     /old_locus_tag="pgaptmp_000469"
     CDS             complement(88410..89072)
                     /gene="ccmB"
                     /locus_tag="ID725_RS02345"
                     /old_locus_tag="pgaptmp_000469"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGV7849150.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(89069..89692)
                     /gene="ccmA"
                     /locus_tag="ID725_RS02350"
                     /old_locus_tag="pgaptmp_000470"
     CDS             complement(89069..89692)
                     /gene="ccmA"
                     /locus_tag="ID725_RS02350"
                     /old_locus_tag="pgaptmp_000470"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGV7849151.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(89705..90307)
                     /gene="napC"
                     /locus_tag="ID725_RS02355"
                     /old_locus_tag="pgaptmp_000471"
     CDS             complement(89705..90307)
                     /gene="napC"
                     /locus_tag="ID725_RS02355"
                     /old_locus_tag="pgaptmp_000471"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGV7849152.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(90317..90766)
                     /gene="napB"
                     /locus_tag="ID725_RS02360"
                     /old_locus_tag="pgaptmp_000472"
     CDS             complement(90317..90766)
                     /gene="napB"
                     /locus_tag="ID725_RS02360"
                     /old_locus_tag="pgaptmp_000472"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGV7849153.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(90763..91626)
                     /gene="napH"
                     /locus_tag="ID725_RS02365"
                     /old_locus_tag="pgaptmp_000473"
     CDS             complement(90763..91626)
                     /gene="napH"
                     /locus_tag="ID725_RS02365"
                     /old_locus_tag="pgaptmp_000473"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGV7849154.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(91613..92308)
                     /gene="napG"
                     /locus_tag="ID725_RS02370"
                     /old_locus_tag="pgaptmp_000474"
     CDS             complement(91613..92308)
                     /gene="napG"
                     /locus_tag="ID725_RS02370"
                     /old_locus_tag="pgaptmp_000474"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGV7849155.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(92315..94801)
                     /gene="napA"
                     /locus_tag="ID725_RS02375"
                     /old_locus_tag="pgaptmp_000475"
     CDS             complement(92315..94801)
                     /gene="napA"
                     /locus_tag="ID725_RS02375"
                     /old_locus_tag="pgaptmp_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGV7849156.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(94798..95061)
                     /gene="napD"
                     /locus_tag="ID725_RS02380"
                     /old_locus_tag="pgaptmp_000476"
     CDS             complement(94798..95061)
                     /gene="napD"
                     /locus_tag="ID725_RS02380"
                     /old_locus_tag="pgaptmp_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGV7849157.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(95051..95545)
                     /gene="napF"
                     /locus_tag="ID725_RS02385"
                     /old_locus_tag="pgaptmp_000477"
     CDS             complement(95051..95545)
                     /gene="napF"
                     /locus_tag="ID725_RS02385"
                     /old_locus_tag="pgaptmp_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGV7849158.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            95654..95818
                     /gene="yojO"
                     /locus_tag="ID725_RS02390"
                     /old_locus_tag="pgaptmp_000478"
     CDS             95654..95818
                     /gene="yojO"
                     /locus_tag="ID725_RS02390"
                     /old_locus_tag="pgaptmp_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HGV7849159.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSLIRI"
     gene            95952..96440
                     /gene="eco"
                     /locus_tag="ID725_RS02395"
                     /old_locus_tag="pgaptmp_000479"
     CDS             95952..96440
                     /gene="eco"
                     /locus_tag="ID725_RS02395"
                     /old_locus_tag="pgaptmp_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGV7849160.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(96590..98236)
                     /gene="mqo"
                     /locus_tag="ID725_RS02400"
                     /old_locus_tag="pgaptmp_000480"
     CDS             complement(96590..98236)
                     /gene="mqo"
                     /locus_tag="ID725_RS02400"
                     /old_locus_tag="pgaptmp_000480"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGV7849161.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(98454..100097)
                     /gene="yojI"
                     /locus_tag="ID725_RS02405"
                     /old_locus_tag="pgaptmp_000481"
     CDS             complement(98454..100097)
                     /gene="yojI"
                     /locus_tag="ID725_RS02405"
                     /old_locus_tag="pgaptmp_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGV7849162.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(100173..100823)
                     /gene="alkB"
                     /locus_tag="ID725_RS02410"
                     /old_locus_tag="pgaptmp_000482"
     CDS             complement(100173..100823)
                     /gene="alkB"
                     /locus_tag="ID725_RS02410"
                     /old_locus_tag="pgaptmp_000482"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGV7849163.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(100823..101887)
                     /gene="ada"
                     /locus_tag="ID725_RS02415"
                     /old_locus_tag="pgaptmp_000483"
     CDS             complement(100823..101887)
                     /gene="ada"
                     /locus_tag="ID725_RS02415"
                     /old_locus_tag="pgaptmp_000483"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGV7849164.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(101961..103016)
                     /gene="apbE"
                     /locus_tag="ID725_RS02420"
                     /old_locus_tag="pgaptmp_000484"
     CDS             complement(101961..103016)
                     /gene="apbE"
                     /locus_tag="ID725_RS02420"
                     /old_locus_tag="pgaptmp_000484"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGV7849165.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(103128..104231)
                     /gene="ompC"
                     /locus_tag="ID725_RS02425"
                     /old_locus_tag="pgaptmp_000485"
     CDS             complement(103128..104231)
                     /gene="ompC"
                     /locus_tag="ID725_RS02425"
                     /old_locus_tag="pgaptmp_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGV7849166.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
BASE COUNT        25493 a        28156 c        25936 g        24899 t
ORIGIN      
        1 acgataaaca gtatgtttgg tgttggggga agtcttacgg ctggactgag tgcgatacct
       61 tcgaagggta ttacgattgt tcgagaaaca aatggtgggc agttactgac aatggggaag
      121 aaccggcatc gaaagtaacc cactggatgc cgctaccgga gccgccgcag gaggtgaaat
      181 gatgaattgg cctgaagcat tcaccgcagt tggagttgca atagcggtgg catttattct
      241 gtattcgctt ttccgctggg gataaaggaa tgttcgctct gattcaacgt ggtcagatat
      301 acacggacag agccggatac cctgtggtga ttactcgcag tactcagcac tcagtgttct
      361 ttcgacgcat ggacgggcgc tccggacggg tacgcattgg tgagttcaac aacctgttcg
      421 aacatattga ccaacaggag taccgcaaaa ttctggcggg cactgagcag gaaatgcgcc
      481 tgaaaaaatt acgcgcaatg caacggaggt gatacatgca tacggctttt gagttctggg
      541 ttcgcaagac attcggcaat cgctacgacc tgaaccgtga tgtcgacggc ttctactgcc
      601 gtgaagttgt gaaacgaatg tttgacgtgt ggtgccactg ccgtggatga aagttttatg
      661 aggttggcat gcagacaatc atctatcaga taacccccag caaatggtgt acggagagag
      721 tccttattgc atcaacaggg ctaaagcccg gcaccatcga gcgggccaga agaaagtcat
      781 ggatgcaggg aaaagaatac cgccattacg ctgtagaagg tgatcctggg cattacagtg
      841 aatgcctgta caacatcgaa gaaattatgc gatggatcga aaaccagaaa caaccaggtg
      901 ccaaaaatgc aagttccggt taacctgtta atgctcctgg acgtctggga ggtttaatga
      961 gtaacgcatc atacccgaca ggcgttgaaa accatggcgg atcactccgt atatggtttc
     1021 actataatgg caaacgtgtc agagaaaacc tcggtgttcc tgacacagcc aaaaaccgga
     1081 agatcgctgg tgaacttcgc acttccgttt gttttgcaat cagaatgggg agtttcgact
     1141 acgccgcgca gttccctaat tcccctaacc tgaaacactt tggtctggga aaaagagaga
     1201 taaccgttaa ggcactttcg gaaaaatggt tggaccttaa gaaaattgag atttgtgcga
     1261 atgcacttaa ccgttaccag tcagtaatta aaaacatgtt accaatgtta ggtgaaaaaa
     1321 aactggtttc atccataaca aaagaggatt tacttttcgt aaggagagat ttgttgaccg
     1381 gttaccaaaa gctttctaat ggaaagactt cttccataaa agggcgctca gtggtcacgg
     1441 taaactacta tatgacaacc atagctggaa tgtttcaatt tgcaacagat aatggttata
     1501 cctcaggaaa cccatttaac ggtctggctc ccttaaaaaa gtccaaggta aaaccagatc
     1561 ctctcacccg tgacgaattt attcgtttta ttgaggcttg ccgtcatcaa caaacaaaaa
     1621 acctgtggat tctcgctgta tacacgggta ttcgtcacgg ggagttggta tcgctggcat
     1681 gggaagatat agaccttaaa gcaaggacta taaccatccg tagaaattat acaaaacttg
     1741 gcgaattcac tccaccaaaa accgatgcag gcaccggaag gacaattcat ctggttcaac
     1801 cagctattga tgctcttaaa agccaggcgg aaatgaccat gcttggaaag caacattctg
     1861 tagaggtgaa gcagagggaa tatgggagaa ctgctgtgca taaatgcact tttgttttta
     1921 gtcctcaggt aacaaaacag cagcagttgt ccggacctca ctacaaggtt gactccatca
     1981 gggagtcatg gacaagtatc ttaaaacgcg caggtctgag acacagaaaa tcgtaccaat
     2041 ccaggcatac ttatgcatgc tggtcacttg ccgcaggagc taatcctagt tttatcgcaa
     2101 gccagatggg ccacacaaac gcacaaatgg tattcaatgt ttacggagca tggatgaaag
     2161 acaacaatca cgaacagata gaactcctta acaaaagact atctgaaagt gtcccatgta
     2221 tgccccataa gaaagcaggg taaaataaaa acttgcaaaa tcaattagtt tacccttaat
     2281 ccctgtcacg ttacgcgcgt ggcagaggcg ttacggattg ctgaaaccgc aacggacaga
     2341 cggcggtcat cggctgttca acgatgccga tattgaccgg atccgcgaga tcaaacgctg
     2401 gatcgacaac ggcgtgcagg tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga
     2461 tgtgcagaac ggctggcgcg atcagcaaga aacattactg acctacctgc aaagcggcaa
     2521 tctgcatagc ctgcgaacgt ggatcaaaga gcgcggtcag gattaccccg cccagacgct
     2581 caccacacat ctgtttattc ctctgcgccg acggcttcag taccaacaac cgactctcca
     2641 ggcgctgctg gcgatcctcg acggcgtact gatcaactac atcgccattt gtctggcttc
     2701 ggcacgtaaa aaacagggta aagatgcgct ggtggttggc tggaatattc aggataccac
     2761 ccgtctgtgg ctggagggct ggattgccag tcaacaagga tggcgcattg atgtcctcgc
     2821 ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc tggtgtggtg
     2881 cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag aacaaggcca
     2941 tgatattttc ccactcggca tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc
     3001 tttcgttaac ataaggagtg cattatgcgc atagctaaaa ttggggtcat cgccctgttc
     3061 ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac ctttattttg
     3121 cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg atcgccgcca
     3181 gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta agcccgatga
     3241 tgatgggcgt acccagcgtg ctggccccta ccgttgaggc gatcgtggcc gtaccaatgt
     3301 tgataatcac cgaggttcgc acgcccgcca gaatcaccgg agccgccagc ggtagctcga
     3361 ccttacgcag tcgctgacct cgactcattc ccataccttt cgcaacttct gtcacgctgg
     3421 catcaatcgc tcccagcccg gcaagtgtcg cctgcaggac gggcagcaca ccataaagga
     3481 tcaaggcgat aatcgctggt tgcagaccaa agcctatcac cggaacagca atcgccagca
     3541 ctgcgacagg cggaaaagtc tgcccaacgg cggcaatagt ttccaccagt gggcgaaatt
     3601 ccgcgcccca cgggcgagtg actgcaattc cggcaccagt gccaatgatc accgcaaaca
     3661 aactcgaaat tcccaccagc cagaaatgag ccagtgccaa agctgcaaaa ctttcttgct
     3721 gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca
     3781 gccagaaaat cagtgccaca aacaaagcaa tgagccagaa cagcggatcg cgcaacatct
     3841 tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtgccgct aggctggccc
     3901 tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga caaacaggga gagcgcatcg
     3961 cgcagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa
     4021 tccgccacac tacgtaacga aagcaggcgc acacccagtt cactacgtcc aaaaaactgg
     4081 cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg ctgcaccact
     4141 tcaccgtgat ccatcaatac cagatgttct gccagccgta gcgcctcatc aatatcatgc
     4201 gtgaccagca caatggtacg ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac
     4261 gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga
     4321 tcggcagcca gtgcgcgcgc tactcccaca cgttgctgct gaccaccgga aagctgatgt
     4381 ggataacgct cacgtaaatt tgactccagc cccagtagcg ccattaattc gtcgatacga
     4441 tcgtcaatcc tcgcccgcga ccatttttgt aattgcggca cggtggcaat gttttgtgcc
     4501 acgctccagt ggggaaacag gccaatagat tgaatggcat agcccatacg gcggcgcaac
     4561 tccagtactg gcagcgagcg aatttcttct ccagcaaagc ggagctctcc gctgtcatgt
     4621 tccaccaggc ggttaatcat tttcagggtg gtggatttgc cggagccaga tgtgccaatc
     4681 agcaccgaaa aactcccttc ctgaaaattg agattgagat cgttaacggc tttttgtgcg
     4741 ccgaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca acagtgcgat
     4801 tagtaaatcg aacagcgcat cgatcagcac cgccagaaca attaccggga tcacccccag
     4861 caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac caaaaccgcc
     4921 tgcgccgatt aacgccgcaa tcaccgccat gcctacagtt tgcaccatca ccacccgcag
     4981 gctgcgcaga aataccggta acgccagcgg taactgaaca tgcaggaatc gctgcgcccc
     5041 gctcatgccc atcgctctgg cgctctccag cacatcgcgc gggatctggt tcaagccgac
     5101 taccacgccg cgcaccagcg gtagcaaggc atagagcacc agcgcaatca gtgcgggtgt
     5161 cattccggtt cctgctatgc caagcttccc cagccacgga aaggccgtaa ccagtccggc
     5221 aagcggcgca atcagcaggc caaagagcgc cacggaaggc acggtctgaa tgacattgag
     5281 cagagaaaaa atcgccccct gccgcgcggt ggaaaagtag caccagatgc ccaacggcac
     5341 accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc
     5401 gtcgtcaaac acatcctgac ggttggcgta ttctttcatt agtgagagat cgttaagcgt
     5461 gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca accagcgcca
     5521 cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg ccagcgccga
     5581 agccaaccag aaaccactgc cgaggctggt acgcgccagc gcactgccat tttgcgccag
     5641 ttgggtcgcc gcctttccag ctccccacac cagcaatacg aagacgaatt gcgccagaat
     5701 gagtgcaaaa atgctgcctt ttttagcggg aataaaacag gctgtcagcc aggcgcaacc
     5761 aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgac gcccttcacc
     5821 cgagactaaa cgattaggcg cgtagctgat aaacggcagc gctgctgcga ttgccgtcag
     5881 caacagcagc agcgccagaa caggattaat acgtagataa gtcacgggaa attacttcgt
     5941 ccaccctttt tgtttcaagt agtcggcagc cacttttttg gcatccagtc cttccactgc
     6001 aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag
     6061 ccactgtgcc atttgcggat actctttcag caccgactca cgcaccactg gtgcaggcgc
     6121 gtagataggt tgcacgcctt gcggatcgct taaggtttgc agcccctgcg ccgcgaccgg
     6181 accgtcagtg ccgtaagcca ttgcggcatt aacgccagaa gtttgctggg cagcggcttt
     6241 aatcgttacc gccgtgtcgc cgccagccag tgacagtaac tgatcctgat cgagtttaaa
     6301 gtcgtaggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact cagccgaggc
     6361 tgccagtttg aaggtgccgc cctctttcag ataacgcctc aggtcagcaa gcgaagtgag
     6421 tttgtttttc tctgccacat cctgacgcac ggcgatggtc caggtgttat ttgcaggtgc
     6481 gggcgtcagc cagattaact tgttttgctc tgcatcgagt tttttgactt tctcgtaacc
     6541 ttgctgcgcg tttttccacg ctgcatcgtt ttcatcttta aagaaaaacg cgccattgcc
     6601 ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca ccacaggagt
     6661 cgttccaagt tgcactttat tgaccgttgg tactccgtgg ctttcgagta cttgcaaaat
     6721 gatattgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa cgggggaagc
     6781 cgcgtgcagc ggcaggctca cggctgccaa caaaaccagt gaacctgccc agacctttga
     6841 gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta agagaaaagc
     6901 atagttgata atggcgggat tagcctgagc ggacggattc agttgcagaa tcagataaat
     6961 acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc gccttaaccg
     7021 gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa gggcgcaaac
     7081 gtcgcgccct tgctaaagca ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa
     7141 tcagtgccga taaagacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc
     7201 cagaacttca gcgcctcgat atcaatcggg aagctgacgg tctgggtttc gcccggcttc
     7261 agggtgattt tctcaaagcc tttcagctgc ttcactggac ggctcatgga agccgtcaca
     7321 tcctgcaagt acatctgcac taccgtggcc ccttcgcgtt taccggtgtt cgtcacctgc
     7381 acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt cacatcagag
     7441 acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc gttagcttca
     7501 tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg accggtgttc
     7561 agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat cggcagctta
     7621 ccggacgggt tgtaatcgcc aaacaacaca tcggcaattg cattaccgcc ttcagtcccg
     7681 gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga
     7741 cgcccgttca tcagcaccag caccagcggt ttgccggtgg ctttcagggc ggcaatcaaa
     7801 tcacgctggc tttgcagaat agtaatatcg gtacggctgg aggcctcgtg cgccatcccc
     7861 tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg caccgcttca
     7921 tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata ctgattcagg
     7981 aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg
     8041 ttttcaccga cggcattttt aatcccggtc agcacggtca cggattgatc ggcaacacct
     8101 gccgccgacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc aaccaccgca
     8161 atggtggctg attttttcag cggtaacgtt tcgagacggt ttttcagcaa caccaggctt
     8221 tcacgcgcca cttcacgcgc ttctttgcgg tgcaggcggc tttcggcatt ggtatccacc
     8281 gggtcagact ctttcggacc gagatggctg tatgggtcgt taaacaaccc catatcatat
     8341 ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatcgtcac tttaccggat
     8401 ttgatcaacc caggcagata cttcgagtag tactcgtcgc tcatgctcat gttgattccg
     8461 gatttcagcg ccacgcgcac tgcatcttcc ggatctgccg ccgtgccatg tttaatcagc
     8521 tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga
     8581 acatctttca gcagccagga gtcggaggtg gctggcgtgc cgttcagcga gttcagcgcc
     8641 accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat ataatcatta
     8701 aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg
     8761 tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg gcttttaccc
     8821 tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc ttcgccaaaa
     8881 ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc
     8941 atattcaggc catcatctgc cgcttcataa gcagagacac gtccgaccgt ttttactgca
     9001 tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc
     9061 agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc
     9121 tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tagccccaac ctgaccgtct
     9181 ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat taagcgcagc
     9241 tgaccaattt tctcatcaac tgtcattttc ttaagcagtt cggtgacgaa cgcatcccgc
     9301 gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg ctgcagggcc
     9361 agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct tcctttgttg
     9421 cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga
     9481 gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat
     9541 acaaggcgct attctagttt gtgatgtttt ttcgccacca caaggagtgg aaaatgtctt
     9601 ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt
     9661 cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc cgttctggtc
     9721 atggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga
     9781 aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg
     9841 aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc agcaccctac
     9901 gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat ccgggcacca
     9961 cgctttattc cctggaaaaa gccctcaaac cgctgggacg cgaaccgcac tcggtgattg
    10021 gttcatcgtg cattggcgca tcggttatcg gcggtatctg taataactct ggtggttcgc
    10081 tggtgcagcg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata aatgaagatg
    10141 gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc
    10201 ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg
    10261 cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg cccgcacgtt
    10321 ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag ctggccgtct
    10381 ttgcagtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca
    10441 ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac ttcgaaaatc
    10501 tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca
    10561 aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc
    10621 tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttactg
    10681 accgtgcgat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa
    10741 actggcgcga taaatacgag catcatctgc tgttaaaaat ggcgggcgat ggcgtgggcg
    10801 aagccaaatc gtggctggtg gattatttca aacaggccga aggcgatttc tttgtctgta
    10861 cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc
    10921 gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcgctggat atcgctctgc
    10981 ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc cagctggtgc
    11041 acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac atcgttaaaa
    11101 aaggcgtgga tgtgcatgcg ttgaaagaac agatgctgga gctgctacag cagcgcggcg
    11161 cgcaataccc tgccgaacat aacgtcggtc atttgtataa agcaccggag acattgcaga
    11221 agttttatcg cgagaacgat ccgaccaaca gtatgaatcc ggggatcggt aaaaccagta
    11281 agcggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat cgccatccga
    11341 attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt
    11401 tttatagctt aacgctgctg ccggaacagg catcacttta ccggtttcaa tccaggtacg
    11461 caggcggctg gcatcggcaa aatgggtgta tttgccaaac gcatccatca ctaccagcgc
    11521 caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg cattggtaaa
    11581 gccggttttg gttagctgga tattccagtt atcgcgatac accagatgat tagtattgcg
    11641 gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg tagtacttaa
    11701 ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt cacgggcggt
    11761 tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt tcattccgag
    11821 cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg gataatggtg
    11881 cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca gcatatcttt
    11941 acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg
    12001 gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca cgaccatcgc
    12061 agtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt tcgaatagat
    12121 cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt ccggttgtga
    12181 agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gccaaaggca cagccagcat
    12241 cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc agatagtggt
    12301 tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc gacgccatca
    12361 taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag gaaatgctgc attgctgaca
    12421 tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc gtagccccat
    12481 agtataacgg taagggcgag cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa
    12541 ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat accaaggtaa
    12601 agtaggtaac cggtgtaaaa cagtgccacc gtgccgacca gcgcacacaa ccagaccagc
    12661 ggataaagcg ccaccagacc acttaaaaac agcggagttg caacgtagcc cgcgaagacc
    12721 atgcagtgcg caagtgacgg acgctgcgga taattacgcg ccatccacca gatgacccgc
    12781 cccatcaccg cgaccccagc gagcatatcg ccataaaaca ggacagccag cgccagtccg
    12841 gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg
    12901 ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg
    12961 tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg atgggaaaac
    13021 agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct gacagttcaa
    13081 gtataagtca gcttgtgatt attttttgtt cgcccatgta atttgcaccg tctgatgtac
    13141 gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca aagggagaca
    13201 gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc gctggttatc
    13261 ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gccttgcggc acatcagggg
    13321 ctattaaagt tcccgctggt ggtactttct gtagcgcttg gcggcatgat tggcgaccag
    13381 gtgctctatc tgtgcgggcg gcggtttggc ggtaagctgt tacgccgttt ctctaaacat
    13441 caggataaaa ttgagcgggc gcagaaactg atccaacgcc atccgtatct gtttgtcatt
    13501 ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg tgccagccag
    13561 ctgccgccga aaatctttct gccgcttaat attctcggcg catttgcctg ggcgttgatt
    13621 tttaccacta tcggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgac
    13681 cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt aggcgtgcgc
    13741 tggtggctga aacgacgcgg gaagaaaaag ccggataatc aggcgtaaaa ccattgccct
    13801 ggataaggcg tgcgcgccgc atccgacatc cggttcacca tgcctgatgc ggcgcttgtg
    13861 cgtcttatca tgcctaccaa aattttaccc attgaaatat ccacacacct cactgcgcta
    13921 ttctgggttg aactgcggat tcgccaacat aaagccgcca tccactatca acgactgccc
    13981 ggtggtgtaa tttgcgccct ccgaacaaag ccacgccacc aggctggcaa tctcatgcgt
    14041 tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt cgctatcatc
    14101 catgccgttc attggcgtag cgatcgcccc aggtgcgact gcgttcacca aaatcttatg
    14161 cctgaccaac tccagcgcca ttgctttggt taacccgccg agcgcatgtt tagcggctgt
    14221 gtaggcgctg gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg
    14281 accaccctgt ccttgtttca ccatctggcg ggcagcaatt tgcgagcata aaaatgcacc
    14341 atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcac
    14401 tttggtcatt gcgcccgcat tattcaccag cacatcaata cgccccagcc gttgaataag
    14461 tttctccagc gcctgcgcac cttctggcag attgccgaga tccagttgca cgatctccgc
    14521 acgtacgccg tggctaacta cctcacgcgc ggtatctttt gccccttctt catctgagtg
    14581 ccaggtaatg ccaatatcaa acccttgctg cgccagtaat aacgcgcact ctttgccgat
    14641 ccccgaatcg gaggcggtaa taatcgctac ctgtgccatc gagttctcca cttaacgctg
    14701 aataaacgtt aagtatagaa gtcacatatc atcagcgttt gtaacccccg cccaacgcac
    14761 cagtgagttg aatggaggca tccagccact gcccttgcaa taacaggcca ttggcccgct
    14821 cacgcagcgc ggggattctg gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa
    14881 agcgggcctg cgcaagacct accacacgca aggcatcgcg ctcaatttgc gcctgatgct
    14941 ggtttttctc cgccagtgtc tgaacctgac tggctgcccg cgccacgtca ttcaccgctt
    15001 caaccaccgc tttgttgtag ctggcgatag acaagttgct ttcggctttt gcgatatcga
    15061 gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca
    15121 tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg
    15181 ccatcaggtt gatgtcagga taaaatgccg cttttgccgc atcaatggtg cttagcgatg
    15241 actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag gagtacccca
    15301 gttcatcagg aagctggctt gccactttcg gcaacgccac cggtttaagc gtcaatgact
    15361 tagtctggtg atttgtgagt gcaattaacc gtgcctcaat aattttcatt ttccccgcga
    15421 catcgttgag ctgctgccgg gttttgctgg cattaatatc ggtttccaca ccttcaactg
    15481 aagaagtaat cccgttctga tatagctggc gatcggtcgc gataatggtg ttctgctctt
    15541 tttcgatttg ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc
    15601 gggctacgct gccagccagc aattggcggg tttgctcgcg ttccgccgcc cgtgctttaa
    15661 ccgtacccag gcgggcagta acctccgccc gattctttcc ccagatatcg agatgccagc
    15721 ccgccgttaa gccaaaagtt ccgttggtgt accacggacc ggtcgtacct gcggccggat
    15781 cgttcagagc aaacggcccc attagccctt ctgccgacat tttttgccgc tccatatccg
    15841 cagaaaagtc gatctgcgga ccatcctgag tagcaactgc cttcgcctgg gcttcagcta
    15901 actgaatgcg ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta
    15961 aggaagtgag ttgattatcg tgatactcca gccaccattg gctgtctggc caaccatttt
    16021 tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcggg cgtttgctgg cttaacgcct
    16081 ggcgggtttc atgcataggc gcacacccgg ccagcatcag taacagcgga aaacaggcga
    16141 tggctggata aaaggaatca cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg
    16201 agattaccgt gtgttgcgat atatttttta gtttcgcgtg gcaatacatc agtagcaata
    16261 aaacgacata tccagaaaaa tatacactaa gtgaatgata tcttccgatt tatcttaatc
    16321 gtttatggat aacggcaaag ggcttcgttt tttcctatac ttattcagca ctcacaaata
    16381 aaggaacgcc aatgaaaatt atactctggg ctgtattgat tattttcctg attgggctac
    16441 tggtggtgac tggcgtattt aagatgatat tttaaaaatc agatatgaca tataagttgt
    16501 caggtcagga actagatatt ttgtgtactt gatttgcaaa cagctccggt gttggtggcg
    16561 tcaaaatcgc tgaagcgttc cccgaaggcc ggggcagccc acatggatgt gggctgaggg
    16621 cgcgtcttac aaggacgttt cctcgcgccc gacccggtag ccgaagggga taagctgaag
    16681 gtaccgcgca gcggcgattt tgttcgccag agcccggggg tgcaggggga ggaggcgatt
    16741 gtcctccccc tgcgcgctcc ttgcgccagt ggcaatatgt ggcttagctc atgaaaggag
    16801 cgcaacaaga tgatgaagct gaataagcga ttcattgttt ctgataaact gcgattccac
    16861 tattatcgca gcttaccgat atcaattgcc tgaatagccc ttgcaatatc aggggaatta
    16921 ttcaacacac gaacatgctg aaataattct gttgcttcat cgtattcttt acgcaaataa
    16981 ctcaaccact gtttaatccg cgctacgtga tataacccgg tatcgccctg cttttccaga
    17041 cgggtatatt tttgcagcaa agcaaccacc tccggccacg gcattcgcgg ttcgttatat
    17101 tttactaccc ggctcaggtt gggaatattg agcgccccgc gaccaatcat cactgagtcg
    17161 cagccgctga tcgccatgca ttcttgcgcg ctctgccagt cccagatttc gccgttggca
    17221 atcaccggaa tattcagccg ctggcgaatc tcgccaatcg cctgccagtc aatatgctcc
    17281 gcgcggtaac cctgctcttt tgtccgtcca tgcaccacca gctccgtagc accagcctgt
    17341 tgaaccgcat cggcgatttc aaatttcttc tcaccgctgt cccagcccag acgcactttc
    17401 acgctgacgg gcaaatgcgc cggtacagct tcacgcatcg cttttgcacc ctggtagatg
    17461 agttcaggat ctttgagtaa cgtcgcccca ccaccgctac cgttaaccgt tttcgacggg
    17521 cagccgcaat tgagatccac gccccaggaa cctaattcca ccgcacgggc ggcgttctct
    17581 gccagccatt gtgggaactg acccaacaac tgcacgcgca ccagcgtacc ggatggtgtc
    17641 cggctggcgt tttgtagctc agggcaaatg cgatgaaaga ctttcaccgg cagcagttga
    17701 tccaccacgc ggacgaactc ggtgatgcac agatcgtagt cgttaacttc ggtcagcaat
    17761 tcacgcacca gagagtcaag cactccctcc atcggagcca gtaacacacg catatcatca
    17821 cccgcaaaaa atgaggcgct atgttagcgc ctcctgtgtt tgcagacaaa ctgacttact
    17881 gattaattcg ggcaagattt ttaccctcac cctaaccctc tcccgaaggg gcgaggggac
    17941 cgcccgtgcg cctgattggg tgaagaagtg cttagcgtgg ttgcaaacag ttatcttctt
    18001 tccagccgta caaccactcc atgccgcgat aaaattctgc ctgcgatttc cctttccaca
    18061 gcagattacg tacctggcgt tctacccctg cggcgtggta aacccgcccc atttcacggg
    18121 tcgaccagac gatgcgcgcg gtgcgcggaa ttcgtaccga ctcatacagc gcaaaggctt
    18181 gcgccgcatc accctcacac tgcgccagcg ccttacccag cgttactgca tcttccagcg
    18241 ccatacaggc tccctgcgcc atatattgcg ccaccggatg ggccgaatcg cccacaagcg
    18301 ttatgcgctt cgttccccac ttcgccaccg gttcacggtc agcggttgac cagcgtcgcc
    18361 atgaagtcgg tttatccaac atctggcgcg gacgggggtg aatcccttca aagtaagaga
    18421 gcacttcctc tttactacca tccttcacgc cccactcttc ctgctggcgg ctgtggaatg
    18481 ttaccaccag gttatactgc ttgccgccgc gcaaagggta atgcaccaag tgacaatgcg
    18541 gccccgccca cagtaccggt gcgttaatgc gcaaatcttc cggcatatcg gcacgatcga
    18601 taaccgcacg gtagacaaca tgtccggtga cacgcggcgc atcgcccaat aagctctggc
    18661 gcaccactga cttcacgccg tcacagccaa tcagaatatc gccggtccag ctattgccgc
    18721 gatcgtcaaa cacggtgaca tcgtcttccg tctcgcgaat atccacgacg ttggtggagg
    18781 tgtgatattc cacgtccgga tgcgtcagta ccgcttccca caccgaggcg tgaatatcaa
    18841 cccggtggat caccgcgtaa gggccgccga aatggtcacg aaaggcctgc ccggtttcaa
    18901 tgcacaccac ttcttcggcg ttcaccgcat ccatcatggt aatgtgatcg gtaaacaccg
    18961 cgcgctggcg ggcaacttcg ccgacgccga ggctgtccag cgccgaaaac gcatttggcc
    19021 ccaactggat gcccgcgcca atctcaccga tctcatgcgc cttttccagc agcaccactt
    19081 tgatccccag gcgggccagt gacagcgcag ttgccgcacc gccgatcccg cctcccacaa
    19141 ttactgcgcg tgtcactttc gtcattatag ttctcctggt caggctgaaa ttttatcttg
    19201 ttgattttct ggcgctgcgg caatgaacgc tggcagctgt gtgcaggcgt cgtacaccgc
    19261 tttgcagcgt ggatacccgc tcaaatcgca gttcatgcgt agcgcgttcg cccactgcgg
    19321 gacaaggcag caatctgcca gcgtcggcgt ctcgcccaca caaaattgcc ccgactggtt
    19381 ctggcgcaac agttgttcca ccgcgcttaa cccttgctgg atccagtgcg cataccaacg
    19441 ttttttttct tcttcactga cgttcagttc ttcggtcagg tagcgcagca cgcgcaagtt
    19501 attcaatgga tgaatatcac aagcgatggc atagacaatt tccagcacct tgttgcgggc
    19561 cggctcttcc tgcggcacca gcggaacttg cgggtagtgc ctctccagcc agtcaataat
    19621 cgccagcgat tgtccgagtg attcgccctc gtcagtcagc agcgtcggca ccagcccgac
    19681 cggattcatc cgccgatagg ccagttcatt ttgctgacca atacggatat tcaccccttc
    19741 ggtctggtaa ttaatgcctt tcagcgccag cgcaatgcgt acccggtacg acgctgaact
    19801 attaaaaaaa ctgtacagct tcatcatgca cctcagacaa tcttcaccgc gataggcgtt
    19861 aagccctcga cgttgcctgt gatcacatca cctttcacta ccgggccaac cccttccggc
    19921 gtaccggtga agatcagatc gcccggttgc agctcaaaga agcctgacag atagcagatg
    19981 gtttcattca ccgaccagat gagatggcga atatcgctgc gctgatgatc ttcgccgtta
    20041 acctgtaacc agattggcgc attatcaata ccgccgatat cgcgggcttt gtgcagcggc
    20101 gcgatgggcg ctgagagatc aaacgcttta ccgatttccc acgggcggcc catctggcgc
    20161 atttccattt gacggtcacg ccgagtcata tcaagacccg tggcataacc ccagacatat
    20221 tcatgggcgt gttccagcgg gatatcgctg ccttttttac caatcgccac caccagttca
    20281 atttcgtaat ggtagttatc ggtctgtgcc ggatacggca gcgccagcgt ttcaccagcg
    20341 gcaaccggaa caacggcatc cgccggtttg cagaagaaga atggcggttc acgatccgga
    20401 tcaaagccca tttcgcgggc gtgagcggca tagttacgtc cgacgcagta aacgcggcgc
    20461 acggggaatt gttcatcact gccaacaacc ggtacggtaa cgggcgcttg cggttcgaat
    20521 acgtattgag tcatttttct ctcctaaatt aataacgcgc ttcgcggaac agccccaggg
    20581 cttcttgtac cggtctgtcc gaaaaactaa ataaaacgga atcttgcgtg gtctggaacg
    20641 acacgccatg ccaggtcggc accacgaaga tatcttttgc actaaagctg aaggtttcat
    20701 tgccgatgat gacctgcccg ctaccttcca ccacatggta gatggtgctg tcagtggtac
    20761 gcgcaacgcg tgaggcaaac cctttcggca gcagttgcag gaaagcgccc atcgacggca
    20821 tcggatagcc gccggtgacc gggttgacgt agcgcatttt gtagccatcc cactcgtcgg
    20881 catcgcccag tcgggttaaa tcgtgcagca cttcgcggct gcggtcataa cgatagttaa
    20941 agatcggcga ggagttcccc gtctgatgcc gcagcggcaa catattggca gcgtaacgcg
    21001 gcagataatc tccctcttta cgcgttaccg gttgttgctc ttccggataa tcttcggcaa
    21061 aaccgcagcc cagaatattc actaatggca gatccagccc atcgagccag ataaccggtt
    21121 cgtcgccagg gttaccgtga tcgtgccagc gccactgcgg ggtcaggata aaatcgcctt
    21181 cattcattgg cgtgcgttcg ccatcaacgg cggtaaatgc ccctttgcct tcaacaataa
    21241 aacgcagcgc cgactgatta tgacgatgac tcggtgccac ttcgcccggc atgatcagtt
    21301 gcaaaccggc atataaagtc gcggtaatgg aagactgccc gcgcagcgcc gggttttcca
    21361 gcaccagcac gcggcgcacg gcctctttcg caccaatcag gccgccgctc tccagtagca
    21421 gcgggcggat ctcctgataa ttccagtacg ctggcgcaca gttagcgttg ggcgttttcg
    21481 gcaccagatg gtgcagtgac tcccacagcg gggtcaggtt ttgcccggaa atatgctgat
    21541 aaaattgctc gcggctattt tgattgttat cagtcattga gatgctcctt attcgttcac
    21601 aacgcctgcg cgaggcagtg agtcagggac agaggtcgac tggcgaacca cggtaatcag
    21661 cagggttaac atcaccgcac tgatcgccgc aggcacagcg ataatgaaaa acagggtgtc
    21721 gaaagagaaa ttcatcgcca tcatcacgcc gccagacagc gaaccaacaa tcgcgccgca
    21781 gcggccgata gcatttgacc agctcacgcc cgtggctcga ctttgtgttg gataaagtgt
    21841 tgcggtcagc gcattcagcc ccacctgcga tccgctaatg ccaatgccag tgccgaaaat
    21901 cgccagcgcc atcaaccata atccatcctg gcttaagcca atcatcacga tgcatatagc
    21961 gcctatggca tagctcagcg tcagcacgcg gaaaggattg aacttatcca tcagcacacc
    22021 cagcgccagc gcgcctaacg taccaccaat ctggaacgcg gcggtgaccc tggacgcgtg
    22081 ttgcaggtcg atgccgcgat ggttcagtag cgtcggcatc cagcttgaga gcaggtaaat
    22141 aatcagcagg ctcataaaga acactaccca cagcatcaac gtgatgggta actgacgtcc
    22201 cataaacagt tgacgaatgc tgccttttgt aacggaggct gattcaatga gataaaagtg
    22261 ggtatcgaca taacgctcgc gggtgatggc gctgacggtt ttcgcaatca ccgcctgcgg
    22321 tagctggcgg cgcacctgcc agcgtggcga ttccggcaga actaccagca gtgcgacaaa
    22381 cagcatcagc ggtaacacgc cccccaacac cagaatgccg tgccagccaa tcaccggcac
    22441 cagctgcgca ctgacaatcc cgccaaacgc cgaacccagc gtaaagccgc aaaacatcag
    22501 cgtcaccagc gcgccgcggc ggcgggcagg cagatactcc gaggtcatgg tgatggtgtt
    22561 cggcatcgcg ccgcccagac ccaggccggt gagaaagcgc aggaagacca gcgtttgcag
    22621 atccggtgag aaagccgagg cgaggcttaa cgcgccaaac aaaaagacgc acagctcaat
    22681 cacccgcttg cgaccaaagc gatcggacaa cgggccacaa agcagtgcgc ctgcggttaa
    22741 tcctagcaga cccgcgccaa aaagcggggc gagatcgcct gcggtgagtt gccagtgagt
    22801 gcgaatatca ggggcgataa atccgatagc cgcggtgtca aaaccatcga gcattaccac
    22861 cagaaaacag cagatgataa cgcgccactg catcttgccg accggagcag catcgataag
    22921 tgcttgtaaa tcacgtcgtt gagtcatagt aaatgcctca gtgcaggtaa ggtagatgtt
    22981 tcttttgttt tgtatatgtt gcgatattgt gattaaagag atgagatgta caatggcatt
    23041 tcgggcagag agataacctt gaggttatgg caaatggcga actgggcgca aaaattaaaa
    23101 ctacatcacc ttgaaatgct ggtggcgctg ggtgaacagg gcaatctaac ccatgtcgca
    23161 cgaatgatga atatcaccca acctgcgctt tcgaaatggt tgtcacaact cgaagatgag
    23221 attggaatca cactttttga gcggcacagc aaaggattac gtccgactga aggcggcaaa
    23281 ttgttattgc aacatgccca acggttaatc aatgacatgg agcgatcgca acatgaactg
    23341 gcccgtttta aacaaggtgg actggtcggc agtctgaaaa ttggctgttc tcccgtcgcc
    23401 actgactgcg tatcccaggc gattttgcat ttgcttacga agatgccaac tctacacctg
    23461 aatatcgaag agaaggtgat gaccccattg ctgcaagatc tattggccgg ttctgtcgat
    23521 gtggtcgttg ggcgaattgg cggtcgcgca ttgcagctgc cactgaatta tcaggtgctg
    23581 tacaccgagc cagtatgctt tgtcgcccgc cctgagcacc cactggcgaa atacgccacc
    23641 ctcagctgga atgatcttgc caactggcgc tggatcgtct ggccgacggg tacccccatt
    23701 cgcattagta ttgataatgc gctggtagac cacggcgtaa tgctgccaga aaacacgatt
    23761 gagtcggcgt cgatgaacgt cagtaccaac ctgttacaaa gcagcgatat gatctctatt
    23821 ttgtcattac gcctggcgca acgttatgcc agccagggac aactggtgat actcaacctg
    23881 ccaaagatag aacagaaagg tagcgtcggt gtgttctggc gcaataacga aacgccttct
    23941 tatgctttaa gtcagtttct gcacttttta cagcagaatt agtgtgctga acggggcgct
    24001 cgcccgaggt tacctcccta aaattcaact atgctgagaa ttcttaatgt gatcggtagc
    24061 acgttttacg aattaattgt atgatgaatc catctcatct ggggtgttga ttatgagtaa
    24121 gacactgaac attatctggc aatatttacg cgctttcgtc ctgatttatg cctgcctgta
    24181 tgcaggcatt ttcattgctt ccctgctacc ggtaaccatt ccgggcagca tcatcgggat
    24241 gctgatcctg tttgtcctgc tggccttgca aattcttccg gcgaaatggg tcaatccggg
    24301 gtgctacgta ctgattcgct atatggcgct attgtttgtg ccgattggcg taggcgtcat
    24361 gcaatatttt gatttgctcc gcgcacagtt tggcccggta gtggtttcct gtgcaatcag
    24421 tacgctggtg gtttttctgg tggtgagctg gagttcgcaa ctggtacacg gtgaacgtaa
    24481 agtcgtaggt cagaaaggat cagaagaatg atggcgaata tctggtggtc attaccgctg
    24541 acgttgattg tcttttttgc cgcccgcaaa ctggcggcac ggtataagtt tcccctgctt
    24601 aacccgttgc tggtagcaat ggtggtgatc attccttttt taatgctgac cggcatctct
    24661 tacgacagtt actttaaagg tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc
    24721 gcgctggcct atcctttata tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc
    24781 atcaccatct gttttatcgg cagcgtggtt gcgatggtga cggggacttc cgtggcattg
    24841 ttgatgggcg cttcaccgga aatcgccgcg tcaatcctgc cgaaatcagt caccacgcct
    24901 attgcaatgg cggttggcgg cagtattggc ggtattccgg caatcagcgc agtttgcgtg
    24961 attttcgtcg gcatcctcgg cgcggtattt ggccatacat tgcttaatgc gatgcgtatt
    25021 cgtaccaaag ctgcgcgcgg tctggcaatg gggactgcct cgcacgccct cggtacggcg
    25081 cgctgcgccg agctggatta tcaggaaggt gcatttagtt cgctagcgct ggtgttatgc
    25141 gggataatta cttcgctgat cgcaccgatc cttttcccga ttattctggc agtaatgggc
    25201 taaaatttgc gatgcgtcgc gcatttttga tgtaagtttc acgcgttgca taattaatga
    25261 gattcagatc acatataaag ccataacggg ttcgtaaact gttatcccat tacatgatta
    25321 tgaggcaacg ccatgcatcc acgttttcaa accgcttttg cccaacttgc ggataacttg
    25381 caatctgcac tggaacctat tctggcagac aagtacttcc ccgctttgtt gaccggggag
    25441 caagtctcat cgctgaagag cgcaacgggg ctggacgaag acgcgctggc attcgcacta
    25501 cttccgctgg cggcggcctg tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt
    25561 gcgcgcggtg tgagcggaac ctggtatttt ggtgccaata tggaatttat tggtgcgaca
    25621 atgcagcaaa ccgttcatgc cgaacaaagc gcgatcagcc acgcctggtt gagtggtgaa
    25681 aaagcgcttg cagccatcac cgttaactac acgccttgtg gtcactgccg tcagtttatg
    25741 aatgaactga acagcggtct ggatctgcgt attcatctgc cgggccgcga ggcacacgcg
    25801 ctgcgtgact atctggcgga tgcctttggg ccgaaagatc tggagattaa aacgctgctg
    25861 atggacgagc aggatcacgg ctatgcgctg actggtgatg cgctttctca ggcggcgatt
    25921 gcggcggcaa accgttcgca catgccttac agtaagtcgc caagcggtgt cgcgctggaa
    25981 tgtaaagacg gtcgtatttt cagcggcagc tacgctgaaa acgccgcatt caacccgact
    26041 ctgccaccgt tgcagggagc gttaattcta ttgaatctca agggttatga ttacccggat
    26101 atccagcgcg cagttctggc agagaaagcc gatgcgccgt tgattcagtg ggatgccacc
    26161 tccgcaacac tgaaagctct cggctgtcac aatatcgacc gcgtgcttct cgcttaagcc
    26221 tggtgccgga tgcggcgtga acgccttgtc cggcttgcca gccctctcct ggtgtcgaaa
    26281 atcccggcaa acagtttgtc gtttcttgcg caaaaccagc gggtaaagta gcctgatgga
    26341 aattttcctt agatcgagtc tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc
    26401 tgatcggctt gctgctgttg actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg
    26461 cgccttatat ctacgacgaa ttgcaggatc tcccctaccg ccaggtcggt gtggtgctcg
    26521 gaacagcaaa atattatcgt actggcgtaa ttaatcagta ttatcgctac cgcattcaag
    26581 gagcgattaa tgcctataac agcggtaagg taaattatct attactgagc ggcgataacg
    26641 cattgcaaag ttataatgag ccgatgacca tgcgcaaaga tttaatcgct gctggtgtcg
    26701 acccatcaga tattgttctc gattacgcag gctttcgtac gctggattcc atcgtgcgta
    26761 cacgcaaagt tttcgatact aatgatttca ttattatcac ccaacgtttc cactgtgagc
    26821 gggcattatt tattgcgctg catatgggga ttcaggctca gtgttatgcc gtaccgtcac
    26881 cgaaagatat gctgtcagta cgtattcgtg aatttgccgc ccgtttcggt gcgctggctg
    26941 acctttatat ttttaaacgt gaaccgcgtt ttttagggcc gctggtccct attccggcta
    27001 tgcaccaggt gccggaagat gcgcaggggt atcccgccgt cacacccgaa cagttacttg
    27061 aattacaaaa gaaacaagga aagtagttat ggatgtacag cagttttttg tcgttgccgt
    27121 ttttttcctt atcccgatat tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc
    27181 gattgataaa cgggttaaaa atgcaccgga accggtgtat gtctggcgag caaaaaatcc
    27241 cggactcttt ttcgcttata tggtggcata tatcggcttc ggaattttat ctatcggcat
    27301 gattgtttat cttattttct atcgttaata ttttctcttt tctgttaatt attgagaata
    27361 attattactt cactcaataa gctgcggata taacattctg aaccgcagct atttgtgaat
    27421 cctttcacag tttaaatttc cccacgcgca tagccttaat atcagcacgc aatatttatt
    27481 tagcgttcgc cttaattact catagcatta aggaagatca catgccgcaa caaaattatc
    27541 tggatgaact cactccggct tttacgccgt tactggcgat taaagaagcc tctcgctgtt
    27601 tattatgtca cgacgctccc tgtagtcagg cttgcccggc acagaccgat ccggggaaat
    27661 ttattcgctc aatctacttt cgtaatttta aaggcgctgc cgagacaatt cgcgaaaata
    27721 atgccctcgg tgccgtttgt gccagagtgt gcccgacgga gaaattatgc caaagcggtt
    27781 gtacccgtgc cggtgttgat gctcccattg atatcggccg cttacagcgt tttgttactg
    27841 attttgaaca acaaaccggg atggaaattt atcagcccgg tactaaaacg ctcggcaaag
    27901 tcgcgattat tggcgcaggt cctgccggat tgcaggccag tgtgacgcta acaaaccagg
    27961 gttatgacgt cacgatttat gagaaagaag cgcaccccgg tggctggttg cgtaacggta
    28021 ttccgcaatt tcggttaccg caatcagtgc tggatgcaga aatcgcccgt attgaaaaaa
    28081 tgggcgtgac cattaagtgc aataacgaaa tcggtaaaac actcaccctg gagcaactga
    28141 aagcggaaaa ccgcgcggta ctggtcaccg tggggttatc aagcggttcc gggctaccgc
    28201 tgtttgagca tagcgacgtt gagattgccg tcgacttcct gctacgtgca cgacaggcgc
    28261 aaggcgatat cagcattcca caaagcgcat taattatcgg cggcggtgat gtcgcgatgg
    28321 acgtagccag cacgctgaaa gttctcggct gtcaggcggt aacttgcgta gcgcgtgaag
    28381 agttagatga gtttccggca agcgaaaaag agtttgccag cgcccgggag ctgggcgttt
    28441 cgatcatcga tggattcacg ccagtagccg tggaaggcaa taaagtcacg tttaagcatg
    28501 tacggttatc gggcgaactg acgatggcgg cagataaaat tattctcgcc gtcggtcagc
    28561 atgccagact ggatgacttt gcgaagttag agccgcagcg taacaccatc aaaacacaaa
    28621 attatcagac ccgcgatccg caagtctttg ctgctggcga tattgttgag ggtgacaaaa
    28681 ccgtggtcta tgccgtgaaa accgggaaag aagccgccga ggcgattcat cactatttag
    28741 agggagcttg ctcatgttaa cgaaagatct ttcgattact ttttgcggcg tgaagtttcc
    28801 caacccgttc tgcctctctt cttcgccggt aggcaactgc tatgagatgt gtgccaaagc
    28861 ctacgacaca ggttggggcg gcgtggtgtt taaaacgatc ggctttttta tcgccaacga
    28921 agtctcgccg cgttttgatc atctggtgaa agaagatacc ggttttatcg gcttcaaaaa
    28981 tatggaacag attgctgaac atccgttgga agagaatctg gccgccctgc gtcggctgaa
    29041 ggaagattac ccggacaaag tattgatcgc ttcgatcatg ggggaaaatg agcagcaatg
    29101 ggaggagctg gcgcgcctgg tgcaagaagc tggcgcggat atgatcgagt gtaacttctc
    29161 ctgtccgcaa atgacttctc atgcgatggg tagcgatgtc gggcaaagcc cggagctggt
    29221 agaaaaatat tgtcgggcag tgaaacgggg ttccacgctg ccaatgctgg cgaagatgac
    29281 gccgaatatc ggtgatatgt gcgaagtggc gctggcggcg aagcgcggcg gcgcagatgg
    29341 cattgcggcg attaacaccg ttaaatccat caccaatatc gatcttaatc agaaaatcgg
    29401 tatgccgatc gttaacggaa aatcgagtat ttccggatat tccggtaaag cggtaaaacc
    29461 gatcgccctg cgcttcattc agcaaatgcg cacccatcca gaactgcgcg atttcccaat
    29521 cagcggtatc ggcggcattg aaacctggga ggatgcggcg gagtttttat tgctcggcgc
    29581 agcaacgtta caggtcacca ccggcattat gcagtacggc tatcgcatcg tagaagatat
    29641 ggcgagcggg ttatcgcatt atctcgccga tcagggattt gattcgttgc aggagatggt
    29701 aggtctggcg aataacaata ttgtcccggc ggaagattta gaccgcagtt atattgtcta
    29761 tccccgtatc aatcttgata aatgtgttgg ctgtggacgc tgttatattt cctgttacga
    29821 cggcggtcac caggcgatgg aatggagcga gaaaacccgc acaccgcatt gtaataccga
    29881 gaaatgtgtg ggttgtctgc tttgtggtca cgtctgcccg gtgggttgta ttgagctcgg
    29941 ggaagtgaag tttaagaaag gcgagaaaga acacccggta acgttgtaaa accagagacg
    30001 catccggcat ttggttcctg tcgctggatg cggcttgaac gccttatccg gcctacaaaa
    30061 ccatacaaat cagtatgttg agctcattgc aggccggata aaacgcgcca gcgttgcatc
    30121 cggcatttgg ctcctgtcgc cggatgcggc gtgaacgcct tatccggcct acaaaaccac
    30181 acagatcatg atgttatgtg cgtttatgac cgaatgcgga ccacaatcat ttcacttctt
    30241 acgcgcgtac ttcagtgaat ccagcgctac ggcgaagata ataatcgccc ctttgatgat
    30301 gtactgccag tatgggttta cgccgatgta cgtcaggcca tagttgatga cggtaaaaat
    30361 aattaccccg gtcaccacgc caatcaccgt ccccacaccg ccgctgaacg atacaccgcc
    30421 taccacgcac gccgcgatag catccagttc atacataaag ccgaggttgt tggtggcaga
    30481 gccgatacgt ccggcttcta acatcccgcc aaaggcatag aacacgccag acaacgcgta
    30541 gatcatcagc aggttcaggc cgacgttgac accagatact tttgccgctt ccgggttacc
    30601 gccaatggca aaaatgttct taccgaagcg ggttttgttc cacaacaccc agacgaacgc
    30661 caccgcaatc aacgcgtaga aggtgatgta agagagacgg aaactcccca gcgcgacaaa
    30721 gccctgagca aaggtagaga agccactgtc aaaaccagaa attggcgacg ccccgacaaa
    30781 gtcgtaataa agcgagttga tgccatagac gatgatcatc gtgccgagcg tggtaatgaa
    30841 cggcgtcacg ttgagataag cgataatcag accgttgatc aaaccgatca ccgcaccaat
    30901 ggcacagaca atcagaataa ccagcgcaat cggcatcgtc gccatttccg ggaacacttt
    30961 gttggcgtta tccatggact gcaataatgt cgctgccacc actgccgcca gccctacttg
    31021 acgccccgca gaaaggtcgg tcccctgggt gacaattaac cctgccacac cgagcgcgat
    31081 aataatacgc accgatgact gggtgagaat attacttaag ttcaacagac ttaaaaatgt
    31141 tggggcctgg aaaataataa tcgcaagcaa aactaaaaga acgacgtaaa taccgccctc
    31201 tttcaggtaa gtaagaaaac ttttcttatt taacgcactc atgggaagcc cctaatctta
    31261 aaggtgcaaa gacgcaagac gtagaatttc gttttgcgtt gttgtttttg tatcgacaat
    31321 tccggaaacg aggccattgc tcataaccag aatacggtct gttatcccta acaactctgg
    31381 catttcagag gagataataa taatcccctt gcctttcttc gccagttcgg caattaactg
    31441 ataaatctca aacttcgccc caacatcaat accgcgcgtc ggttcatcga gcattaatat
    31501 ttctggttgc gttaacagcc agcgcccaat aatcaccttt tgctgattac cacctgagag
    31561 cgaaccaatt tgcgtccgat gccccggtgt ttttacccgc atcgaatcaa tcacccactg
    31621 ggtatcgcgt ttcatccgcg agttatccag taaaccaact ttatttttgt agttgcgaat
    31681 attggaaatt aacgagttaa aaccaatatc cagataggca taaattcccg ttgagcggcg
    31741 ctcctcagtt accagtgcaa atccgtggtt tatggcttcg ttggcattat ggttattgat
    31801 ctttttgccg tgcaacgtaa tggtgccagc cgatttctcg cgaataccaa ataacgtctc
    31861 aacaatatcg gtacgtttcg cccccaccag accggcaata ccgaggatct cccctttatg
    31921 cagatcaaac gagacatcgc gaatcgacgg ctggcgcagt gacgtcaggt tacgtacctc
    31981 gaggatgact tcgcccggct tgttttcttt gtcaggaaaa cgctggttaa gagaacgccc
    32041 aaccatcatg gcgataatct tgtccatcgt cagccctgcc agcggttcgg tggcgatcca
    32101 ctgaccgtcg cgcaatacgg taacttcatc acataactgg aagatttcct ccattttatg
    32161 cgagatataa acaataccgc agccgcgctc ttttaattta cgaataatag tgaacagatg
    32221 attgacctct ttttcggtta acgaagaagt cggttcatcc ataatcacaa ttttcgcgtt
    32281 ataggaaaac gctttggcga tttcgatcat ctgcatttgc gaaacagata atgtgccgac
    32341 gcgcgcacgc ggatcgatat caatatccag ttcatcaaaa atcgctttgg tttcgcggta
    32401 cattttgtcc tgatcgacaa acatgccttt ggtggggtat cgcccaagcc acatgttgtc
    32461 catcaccgaa cgttgtaata ccaggtttaa ctcctggtgt accatcgaaa taccattttc
    32521 cagtgcttct ttggccgaat ggaaatcgat ctctttaccc tggaataaaa tggtgccgga
    32581 gtctttttta taaataccaa acaggcattt taataatgtt gattttcctg cgccgttttc
    32641 ccccattaat gcatggatag aatgtggccg gacttttaaa ttaacgttat caagtgcctt
    32701 aacaccagga aaagacttgt tgataccgct catttccaac aagtattccc cggaagacgg
    32761 agtcgttgag ctgaccatat aattttacct tgttggccat acaataaggg cgcagtaata
    32821 gactgcgccc aatcagtctt atttcttgct gaattcagcc aggttgtctt tatctacgcc
    32881 aacataaggt acgcggacca ctttgttgtc gattttccag ttggtgccat cagccgcacc
    32941 tttaccatcg gccaggtttt tcgccagatc aaaggtcgct ttcgcctggt tgttagcatc
    33001 gttcagtacg gtgcccgcca gtgcaccgga tttcaccagc gccagcgctt ctggcagagc
    33061 atcgacgcca aacaccggaa tgctggactt gttgtgtgct ttcagtgctt ctaccgcgcc
    33121 cattgccatc gcatcgttgt tggcgataac cacttcgatt ttgttggcgt tcgggccaga
    33181 cagccaggcg tccatcttat ctttcgcctg agcggtatcc cacatagcgg tatctaactg
    33241 taactgttca gttttaatgc ctttgtcgtt cagctcttta atcacgtaag tggtacgtgc
    33301 ttctgcatcc ggatggcccg gttcaccttt cagcagtacg aattgaatct gaccgtcttt
    33361 attcagatcc caaccctgat tcgccgccca gtgtttagca atcaaatcgc cttgaataat
    33421 gccggactct ttggagtcag tgccaacgta gtaggctttg tcgtagctat ccagcgcctt
    33481 acgagacggt tctttgttaa agaataccac cggcacattt tgcccacgcg ctttctcaat
    33541 caccgtaccc gcagctgccg ggtcaaccag gttgattgcc agtgccttca cccctttcgc
    33601 cagcaatacg tcgatctgat cgttctgctt ggactggtca ttctgtgaat cattcatcag
    33661 cagctgaaca tctggcgcgg ctttcgcatc ttgctcaata gccttgcgca ctacagacat
    33721 aaagttatcg tcgtacttat agattgttac accaatgcga gtatcagcag cgtgtgcagt
    33781 ggcaccgaat aacatgctgg ccatcacagc agacagggtt aacaccttct tattcatggt
    33841 atctccggtt tttcttatgc agggtagtgc ttgagataaa tgctcggcgg ggcagtagag
    33901 ttaatgaagt gttactgaac gccgaagctc actttttaaa attcgttctt ccatgcccgg
    33961 taacgctcca gaaaacggct ttaattgttg ttatgacgca ttacctcggt gaaagtgatt
    34021 attcactgtt acatacgggt tacaacgtta aaacggtgca atcatagcta tcacattgtt
    34081 aagatactgt gaaatcactc acagattgaa agcggttaca tcgcctgatt tgttgagtta
    34141 gtgatcgccg ccgcattctg gcgtgttgcg acagaatggc gacgcactaa cgtcggcata
    34201 aaacagtggc tggcacgagg atcaatattg cctgctgccc cctgcaaggc cagttcggtg
    34261 gctaatttcg ccattgaagc aatgggataa cgcacggtcg ttaattgcgg gtcggtgtaa
    34321 cgggcgatgg gaatatcatc gaaaccgatg attgagagat gtaacggaat cgcaatgcca
    34381 ttatctttta atgctgtcag tgcgccagca gccatattgt cgttataagc aaatacagcg
    34441 gtaagttgta gattgcgccc cagcagttca accatcgccg cctcaccgcc cggcatgtcc
    34501 ggcgtaccag tgccaatcca gctttccggc ggaataatat cctgctcttt caacgcactc
    34561 atccagcctg ctttacgcat ggcgtcatct tcaatgccgt ggctggaaga aagataacca
    34621 atacgttgat gaccgttatt cagcaacatg cgcgtcgcca ttcgggcacc gctgagatta
    34681 tccaggcaaa cgcaacgatg ggtgtacccc ggcacaacgc ggttgattaa caccataccg
    34741 ggaatgttat ccataaattg cgccagttca tcgtcactca atgcttttga gtgaacaatc
    34801 aacgcattac aacgctggcg aattaacacc tcaatggcgt gacgctcttt ttccgcttca
    34861 tgatagctat tgccgattag cacgtatttc tgatgctgct gagcgaccag atccaccgct
    34921 tttaccagcg cgccgaaaaa cgcatcagaa acgtccatca ccaccacgcc aatggtgtcg
    34981 ctaacctgag ttgccagcgc ctgggcattg gcgtttggcc gataatccag ctcactcacg
    35041 gctttcatta ctgcttcacg cgtgtcggca ctgaccagcg tgctgttatt gagcacccgg
    35101 gaaaccgttg ccacagagac gccagcctga cgcgctacat cacgaatggt gatcatattc
    35161 actacctgtt atgagattgc agtaaatgac tgcttgctgc cggctatttt gtcagcactg
    35221 aatacaggac ttcgtgaatc gagtcacagc aatggaaacg gttacagccg ttttgttaac
    35281 gaatgtgatc cagatcgtta tcttgatgtt ttagatattg ccggtccggc agcacgcaaa
    35341 gttaactgac gccagagcca ttccaccggc ccctggcgga agtaacgcag ccagataaca
    35401 gagaagagaa tattcgccag ccataccgga ataacaaacg ccagcaattc cagacggtca
    35461 aactgcataa acagaccgag gtggtaaaaa agcgtggtac aaatcagcgt ttgcaataga
    35521 tagttggtca gcgccatccg tccgacgcag gcgatcgcca gcaccagctt aaagcggctc
    35581 aattgcggcc agaagccata aaacagcgac gcatagccaa tcgcctgaaa cggcgcactc
    35641 agttcccgcg gcatttgaag taagaaggca caccagcgat acgcccagtc cagctgccat
    35701 tgcagggcga tggcaggaag gttaatgatc accccaatcg ccactagcac aaaaccagta
    35761 cgacgatagt gacgtaagct gaactgccct ttcagccagc cactgcgcat caatgcggca
    35821 ccaatgagca tcatcccagc cagttgccag ccatactgtg cgcccagtgc caataaactg
    35881 ttcccaacac catcggcacg attactgatc gcttcaacgc cgccgtgaag cttccagtat
    35941 ttttcatata aaatagccga tgcatccggc gtccaggcgc ggctagtctg gctatcggaa
    36001 atcaacccca ataacagcaa aacgccaagc cccaccagat aaagcatgac gccggtatta
    36061 aaaaggcttt ttaccgatgg cgcatcgcgc accagccgcc agcagattaa gcccaccagc
    36121 ccgtaagcca gcagaatatc gccgtcccag aacaataaac cgtgaataaa gcccagcaag
    36181 actaacagcg ttaaccgcga ctggatccag cgtctgccac ggggcagcaa catttgcagg
    36241 ccagcaccaa acagcagcgc aaaaagcgtg aggaatttca cctggccgat gagatcgaga
    36301 aatgcccagg tccatgcatc ctgcggcgta atagcgccgt accaggcggg attaagataa
    36361 gccgcctttg gtagcccaaa ggcgctgatg tttaatagca ggatccccag aatggcgacg
    36421 ccgcgaacaa agtcgagcgt gacgttgcgc tccatggttc ctgcctttaa atcagttgtg
    36481 atgacgcaca gcgcgcagaa actcgtggcg cgtattctga ctggatttga acaatccacc
    36541 aagagaggtc gttgtcgtgg cactggttgc atcgcggatg ccacgcgcct tcacgcaata
    36601 atgcaccgcg tcgatagaga cagccacgtt attggtgccc agcagcgttt gtagcgcaat
    36661 gagaatttgc tgcgtcagac gttcctgcac ctgcggacgc tgggcaaaga actgcacaat
    36721 acggttaatt tttgacagac cgatcaccga atctttcggg atataagcca ccgtcgcttt
    36781 gccatcgatg gtaacaaaat ggtgttcaca ggtactggtc agagtgatat cgcgcacggt
    36841 gaccatttca tcgaccttca ttttgttttc aatgagggtg attttcggga agttggcgta
    36901 atccagaccg gagaaaattt catcgacata cattttagcg atgcgatgcg gcgtttccat
    36961 caaactgtca tcagccaggt cgagattcag cagctgcatg atttcggtca tatgaccagc
    37021 aataaggctt ttgcgcgttt cgttatccat ttcatgcacg ggcgggcgca gcggtgtttc
    37081 cagtcctcgc gcaactaacg cttcatgaac cagggccgct tctttactga gtgatggcat
    37141 ttatgatttc tcctgcaggt gtgacgcctc cgccctgcgt gggggcaaag ttattaagct
    37201 gatttacagc ctgattattg tgcgtgaggc ggcgcacata atccagtatt cacagcgata
    37261 attattgtaa ttgccgctgc ctttcatcag cagatgttaa aacatcgtta tgcaaatacg
    37321 gaagtgaaag ttactcacag cacattgaat aaacggtatg atgaagaaat tgcaaacaac
    37381 acaacaagga gccacgcatg gaaatgctcg aagagcaccg ctgttttgaa ggctggcagc
    37441 aacgctggcg acacgactcc agtaccttaa actgcccgat gacgttcagt atctttctcc
    37501 caccccctcg tgatcacacc ccgccaccag tgctgtactg gctttccgga ttaacctgca
    37561 atgacgagaa cttcaccacc aaggcgggtg cccagcgcgt ggcggcggaa ctggggattg
    37621 tactggttat gccagacacc agcccgcgcg gcgaaaaggt tgccaacgac gatggctacg
    37681 atttaggcca gggcgcaggc ttttatctta atgccacgca accgccgtgg gcgacgcatt
    37741 accggatgta tgattatctg cgtgatgaat taccggcgct ggttcagtcg caatttaatg
    37801 tcagcgaccg ctgcgctatt agcggtcatt caatgggtgg tcacggtgcg ctgattatgg
    37861 cgctgaaaaa cccgggcaaa tacaccagcg tttcggcctt tgcgccaatt gtgaatccgt
    37921 gcagcgtccc gtggggaatc aaagcgttta gccgctattt aggtgaggac aaaaatgcat
    37981 ggctggaatg ggacagttgc gcgctgatgt atgccagtaa cgcgcaggat gcgatcccga
    38041 cgcttatcga tcagggtgat aacgatcagt ttcttgctga ccaactgcaa cctgcagtac
    38101 ttgcagaagc cgcgcgccag aaagcatggc cgatgacgct gcgtattcag ccgggatatg
    38161 atcacagtta ctacttcatc gcctctttta tagaggatca cctgcgcttc catgcgcagt
    38221 atttactgaa gtgaaagtcc gcccggttcg ccgggcatct tctcatcaga agcgataatc
    38281 cactgccata aagtaacgac gtccgtcttc gttatagctg tagtcgtcac gactgagatc
    38341 cttgtcgcca aggttcagca cgcctgcgcg cagtttgacg tctttagtca cctgccaggc
    38401 cgcgccggta ttccagatgg tataaccgcc cggtgtttta gccgtcgcgc tgtcggctcg
    38461 tttctgcccg gtatagtgac cagaaacata gaatgaccag tcttccagcg ccagcggttt
    38521 ccagtccagc gtaccgttag cggtatggaa cggcagatcg gatagcggtt tgttttcgcc
    38581 gttgctgaca tcacgaccat cgttgtaggt gtagttgagt gacagtttcc attcatcgtt
    38641 gaacggaatt ttcagctcgg tttccacgcc ctgaatacgt gctttgttaa cgttgtagta
    38701 gctaaatacc ggcacaccgt tcgctgtaaa accaacaaag ttttggtagc ccggtgcagc
    38761 attgacgtca gacgtacggc tgatgctgat acgatctttc acatcgttac ggaaaacggt
    38821 aacgctggat tcaacccctt ccagccagcc ttcttcaccc atgtagtaaa gccccagctc
    38881 ccaactttcg ctggtttctg gtttcagatc cgggctaccc acaatcttac atgcgccacg
    38941 gcaggaattg ctcgtccagt cagggctaag ttgcaacaga gaaggtgctt taaatgccgt
    39001 cgcccagccc cctttcaccg ttacggtgtc ggtggcgtta taaaccaggt aggcacgcgg
    39061 actccagtgt tcaccgtagg tttcgtgatc gtccatacgc acgccggtcg tcagcgccag
    39121 cggctcgaag atccgccatt catcttccac aaacagcgcg tactggctgg cagacgtttt
    39181 ggagctggtt cccccggtca ggttcaccgc atcgctaagt ttgtcgtgac gccattcacc
    39241 gccaaccgtg agaaactgat taatcgccgt cagcggcaac gtgtatttgc cgtcgaccgt
    39301 attgctttcg gaagttatcg ggctgctgtt gccagggttt ttgttctcga ctttctcacc
    39361 gtagtatttc agttcgctgg tgccgtaatc ccaacgccca ttatggctga cggagtagtt
    39421 ctggcgttcc aggcggtttt tgtccagcga gtcggaatca cgatcctgac ggtcgaaacc
    39481 gtatccggca gtaaaatcgt gattttgatt cggtgtccag gcaaattcga cattgccgtc
    39541 gcggctggag aatccttcaa tacgcggcgt ttctccggta tcggtggtcg ttgagttttg
    39601 cgggtcatcc ttttcacgtt ttgccaggct gccgtaagct ttcattccca gcacaccatc
    39661 aattaatggt ccactggtaa agaactgacc gttataggta tcaccgcgat cgcgatgttc
    39721 ctgaatggtg gtatcgacgg taacggtacc cgaccatttc tgaccgattt ttttggtgat
    39781 gatattcact acaccgccga gcgcatcgga accgtacagc gacgacatcg ggccacggac
    39841 cacttcaata cgttcgatgg aatcgaccgg gatccagttc agatcgaaat cattgtggcg
    39901 gaagacggca ttgcgggagt taacgcgttt accgtcgaca agaatcaggg tgtagctgct
    39961 gtccagacca cgaatactaa cgcccttacg gttatcccct tcgttcgtca gttgtacgcc
    40021 aggcacttct ttgaggacat ccttcagatt ctgtaccggt tttcgctgca ggtcttcctg
    40081 ggtaatgacg ctgatactgg cgggtgcatc tttaaggttt tgttcaacgg aagatgcagt
    40141 gacaaccatc gtttcgccat cgtcatcgac cgctaacaca ggccatgcac aagaaatagc
    40201 ggacaaacac agcccgaccc gtacgaaagg gttcaaccta aacattccat atctccatga
    40261 ggtaactacg aaaataaaat gggttatcgc tcacatcttc ttcacgtccc cttgcgtacg
    40321 gcagcatcgc ggtggactta tttgtatgca ggtgatcatc ccagaaagcc ggaccagcct
    40381 ctgatttgtc agcctttttt gattgcggcg taacgataat gcaaacgata acaattatca
    40441 atccaaatgt taaattttat atccgcatga tttgtcaggg aaatggaatt gaaaaagccc
    40501 tctcggttga gagggcttag caaggaaggg agaaacttat ttcttatcgt tctgcgggaa
    40561 cttcatttcg ctgtagcgta cgaagtgggt tcctttaatc agcttgtagc cgaaccaaat
    40621 aatcaggaac agcgggatac caatatacgt tgccgctacg ccgccccagt caatggtatc
    40681 tttcaggaac gcttcgtagt tctggcccaa agtgataatc agacacagaa tgaatgcgaa
    40741 gatcggcccc aatgggaaga aacctgaacg gtacggcaga tcgttaatgt cgtgtccctg
    40801 caatacgtaa ccgcgacgga agcgatagtg gctaatggca atccccagcc aggcgataaa
    40861 accggtcatc ccggaggtat tcagcagcca caggtatacc gtctggttgc caaacatgga
    40921 ggtcaggaag cacagaccgg caatcaccgt cgtcgcatac agcgcattac gcggcacgcc
    40981 accacgcgac agtttagcga aaatgcgcgg cgctttaccg tcacacgcca gggtgtacag
    41041 catacgagta gacgcataca taccggagtt acccgctgac agcaccgccg tcagaataac
    41101 tgcgttcatc accgccgcag cagagagcag acccgcgtgc tggaacacca gggtgaacgg
    41161 actaacgctg atgtctttaa catcgttacg cagcaggctc ggatcggtgt acggaataat
    41221 caggctgata atcaggatcg cgaacacata gaacaacagg attcgccaga acacctgacg
    41281 taccgcgcgt ggaatgtttt tccccggatc ttcggactcg cctgcagcaa taccgatcag
    41341 ctcggttccc tggaaagaga agccgacaat catagctacg ccgatcatcg ccgcaaaacc
    41401 accagcaaac ggcgcttcgc cgattgtcca gttgctccag cccgcaggct gcgcgccttt
    41461 gaagataccg ataatcatca gcacgccaac gatgataaag acaataactg tcgtgacttt
    41521 gatcagtgag aaccagtatt ccgcttcacc aaagccgcga acagagatgt agttcagcag
    41581 gaagataacg ccgaggaaca acgcactcca gatccagccc ggtgtatccg ggaaccacca
    41641 gctcatgacc agctgagctg caaccaggtc aacggcaata gtcaccgccc agttgtacca
    41701 gtagttccag cccagcgcga agccaaagcc ctcttcaaca tagttctgac cgtaagtggc
    41761 aaacgaaccg gaaaccggca tatatgcagc cagttcaccg agactggtca tcaggaagta
    41821 aaccatcagg ccaatcaaca tatacgagag caatgccccg cccggacctg cctgagaaat
    41881 cgttgcgcca gaggcaacaa aaagacctgt accgatggaa ccgccaatgg caatcatcgt
    41941 caggtgacgc gcctttaatt cacggcgtaa gcccggcgct tctgtggttt tagtttcgga
    42001 aaccatacaa aaatgctatc catctaaaaa atgaggcgcg attgtagcag acgattggca
    42061 atccttccgg cagaaataca cggttataag acaccttcat gatcgcccag ggattataag
    42121 taaagcagcc aatctctttt ctggcgaatg catgctgaag gcgctgcgtc acgaaatcga
    42181 cacagcacca gcatgttctt gtacagcaac ttaacgcggc acatttgcgg gatcgcaata
    42241 gtccagaaag cgccgtagcg cgttggaaag gtgtttttgc cgatgatgta tacgccacaa
    42301 cgtacgcatc aggcgcggca gagggaccgc aacttcactt aatgtgcctg cctgcaattg
    42361 atcttcaatc acacgtcgcg acaggcaact aattcccaac ccatgacgca ccgcatgttt
    42421 gattgcctcg gagttaccta attccatcgc catctcaaac ttcggtaaat gtgacagcaa
    42481 cagataatcg acaatctccc gcgtgccgga accgcgttca cgcaggatcc acggcgcagc
    42541 ggccagctgt tctaaggtga ccggaccacg ggccaacggc gaagtcggcg cggcgaaaac
    42601 caccagctcg tcttccagcc acggttcaga aatgatttca gtgctgtggc acggtccttc
    42661 aataaagcca atatcaacgc ggaaatccag caccgcttgc atcacgtcct ggctattccc
    42721 aacgctaagt tcaatcggca actgcggata atcatggcga taacgggcga taactgcagg
    42781 cagaatgtag ttaccgatgg tactactggc atagatacga atcgcgccgt tgtcttcgcg
    42841 aaacagttgt tcgatttcaa ccgcctgttc aagcaatgcc aacgcacgcg gatagagcag
    42901 ccgcccgtgt tcattaacaa ccagtctttt ccccacgcga tcaaacagtt gcacgccaag
    42961 ctgcccttcc aggtcggtca aggctgcgct cactgctgat tgcgacaacg ccagcatcac
    43021 cgacgcttgg gtggttgatc cacttttcaa tacttctgca aaaacttcca actgccggag
    43081 ggtgatgtgc atagtcgctt accacttata aagattaatt ataaatatat aatcaatttt
    43141 atttttaaac cagttagtcg taaccttatg cccggttaaa ggagagggtt atgacgaata
    43201 tcaccttaca gaaacaacat cgtacactgt ggcattttat tccggggtta gccctgagtg
    43261 cagttatcac cggggtcgcc ctgtggggtg gttccattcc cgcggttgcg ggtgccgggt
    43321 ttagtgccct caccctcgca atcttgttgg ggatggtttt aggcaacacc atctatccgc
    43381 acatctggaa aagctgtgac ggtggtgtgc tgtttgccaa gcaatattta ttacgtctgg
    43441 gtatcattct ttatggcttc cgtctgacgt tctcgcaaat tgccgatgtc ggtatcagtg
    43501 ggatcatcat tgacgtgctg acgctgtcca gtaccttcct gctggcttgc ttcctggggc
    43561 agaaagtgtt tggtctggat aagcacacca gctggttgat cggtgccggt agcagtatct
    43621 gtggtgctgc cgcggtactg gcgactgagc cggtagtgaa agcggaagcc agtaaagtaa
    43681 ccgtggctgt tgcaaccgtt gttatcttcg ggaccgtcgc gattttcctc tacccggcga
    43741 tatatccgct gatgtctcaa tggtttagtc cggaaacttt cggtatctac attggttcta
    43801 ctgtgcacga agtggcgcag gtggtggcgg caggtcatgc catcagcccg gatgcggaaa
    43861 acgcagcagt tatttccaaa atgctgcgcg tgatgatgct ggctccgttc ctcatcctgc
    43921 tggcggcgcg tgttaaacag ctgtctgggg cgaacagcgg cgagaaaagc aaaatcacta
    43981 ttccgtggtt tgccatcttg ttcatcgtag ttgccatctt taactcgttc cacctgttac
    44041 cgcagagcgt ggtgaacatg ctggtaacgc tggatacctt cctgctggca atggcgatgg
    44101 cggcgctggg tctgaccact cacgtcagcg cgctgaaaaa agctggggcg aaacctctgc
    44161 tgatggcact ggtactgttt gcctggctga ttgttggtgg tggtgcgatt aactatgtga
    44221 ttcaaagcgt catcgcataa accactacat cttgctcctg ttaacccgct atcattaccg
    44281 ttttcctcca gcgggtttaa caggagtcct cgcatgaaat acattggagc gcacgttagt
    44341 gctgctggcg gtctggcaaa tgccgcaatt cgcgccgccg aaatcgacgc aaccgcgttt
    44401 gccttgttca ccaaaaacca acgtcagtgg cgtgccgcac cgctcacgac gcaaaccatc
    44461 gatgaattca aagccgcctg tgaaaaatat cactacacat cggcgcaaat tcttccccac
    44521 gacagttatc tgattaacct cggacatccg gtcactgaag ctctggaaaa atcgcgcgat
    44581 gcctttatag atgaaatgca gcgttgcgaa cagctggggc tttctttgct caacttccac
    44641 cctggcagcc atctgatgca gatttcagaa gaggattgcc ttgcgcgtat tgccgaatcc
    44701 atcaacattg cgctggataa aactcaaggt gtgacagcgg tgatagaaaa caccgccggt
    44761 cagggcagta acttagggtt taaattcgaa catctcgcgg cgattatcga cggcgtggaa
    44821 gataaatccc gcgtcggcgt ctgcattgat acctgccatg ctttcgctgc cgggtatgat
    44881 ttgcgtactc cagccgaatg cgagaaaaca ttcgcggatt ttgcccgtac tgtcggcttt
    44941 aagtatctgc gcgggatgca ccttaacgat gcgaaaagca cctttggcag ccgcgttgac
    45001 cgccatcata gcctcggtga aggcaatatc ggtcatgatg cgttccgctg gatcatgcag
    45061 gacgaccgtt tcgacggcat tccgctgatc ctcgaaacca tcaacccgga tatctgggca
    45121 gaagagatcg cctggctgaa agcgcaacaa actgaaaaag cggtagcctg aagatgaata
    45181 accgggaaaa ggagatcctt gcaattttac ggcgtaaccc gctgattcag cagaacgaaa
    45241 ttgcggacat gctgcaaatc agccgttcgc gcgttgcggc gcatattatg gatttaatgc
    45301 gcaaaggccg gattaaaggc aaaggttaca ttctcaccga gcaggaatac tgcgtagtgg
    45361 tggggacaat caatatggat attcgcggga tggcggatat ccgttacccg caatcggctt
    45421 ctcatcccgg tacaattcat tgctcagcgg gcggcgtggg acgcaacatc gcccacaatc
    45481 tggcgctgtt aggccgtgac gtccatttgc tttcagtgat tggcgatgac ttttatggcg
    45541 aaatgctcct ggaagaaacg cgccgcgccg gcgtgaatgt ctccggctgc gttcgtttac
    45601 atggtcaaag cacatcgacg tatctggcaa ttgccaatcg agacgatgaa accgtgctgg
    45661 cgattaacga tacccatctg ctggaacagt tatcgccgca attattgaac gggtcgcgcg
    45721 atttacttcg tcatgcgggc gtggtactgg ctgattgcaa cctgacagcc gaggcgctgg
    45781 aatgggtctt taccctcgct ggtgagatcc cggtgtttgt cgataccgtt tcagaattca
    45841 aagcgggcaa aatcaaacac tggctggcgc atattcacac cctgaaaccc actttaccgg
    45901 agctggaaat tttatgggga caggcgatca ccagcgatgc tgaccgtaat gctgcagtga
    45961 atgcgttgca tcagcaaggt gttcagcaac tgtttgttta tttgcccgat gagtcagttt
    46021 attgcagcga aaaggatgga gaacaatttt tgctgaccgc accagcgcat acgacagtag
    46081 acagttttgg tgctgacgat ggttttatgg cgggcctggt atatagcttt ctggaaggaa
    46141 acaatttccg tgacagcgcc cgttttgcga tggcctgcgc ggcaatttca cgcgccagcg
    46201 gcagcttaaa caaccctacc ctgtctgccg ataacgcgct ttcattagtg ccaatggtgt
    46261 aacaatgttg ccggattcga cgcttgaccg catcttatcc ggctcataaa tcgcacacta
    46321 cgctaaacca ataaagaacc cggcaatggt ggcactcatc aggttagaaa gtgtcgccgc
    46381 cgccagcgcg cgtaaaccaa gctgggcgat ttccggcgca cggtgtggcg caaccgcaga
    46441 aaacgccccc accaccaccc cgatagaacc aaagttagcg aaaccgcaca acgcgaagga
    46501 aataatcgcc acggttttag catccagagt gccagccgtt tgcagatagg gtgagaaatt
    46561 gagataagcg acaaattcat ttattgccag tttctgtcca atcaaactcc cggcaagatt
    46621 cgcatcactc cagtcaacac ccattaccca cgccagtggg gccagcagat aacctacaat
    46681 ggactccagc gaggcatgtt caaaaccaaa ccagccgcca acgccgccga taataccgtt
    46741 aattaacgca atgatggcga cgaatgccat cactactgtc gccacacctg cggcgatttt
    46801 cagcccggtc attgcccctg tcgcggcggc ttcaataatg ctttttggcg gtgtttcggt
    46861 gaaagagagg ttattaaaag aaacctgcga agattccgta gccgggctta gcaggcgggc
    46921 aaacaagatc cccccgggga tcgccattaa cgatgccgcc agcaaatatt ccacaggcac
    46981 gcccagtgcg gcataaccaa tcattgtcga accagcaatc gaggccatgc cgctacaaat
    47041 cgctgtaaat aattcattgc gattcagccg atcgataaag ggcttcacaa tcgccggaat
    47101 ttcgttttgc ccgaggaaaa tggtagtgac cgcgacgaat gactcaatct tgctgatatt
    47161 taatgctttc tggaatatac cgccgagaat gcgaattaaa atccccatca caccgatgta
    47221 gtagagaata ctcaccagtg cagtgacgaa gataattgcc ggtaataccc tgaaaccaaa
    47281 gataaatcct gcgccatcaa ataaggtgtc cattttcggt ccgaccagag aaccgaagat
    47341 aaatgcgcta cccgcgtcgc tgtacgccat cactttatgc acaccaaaag cgactttttc
    47401 agcgacccaa cgccctggcg gtaaccaaag cataatgccg ccaatcacga cctgtaacac
    47461 taacgccgcg ccaacggtac gcaggctgat cttcttcttg tttactgaca gtaaaaacgc
    47521 aatcgtcagc aataccacca ttcccagaac acttctcatg acatccatag tgattatctc
    47581 ttcatgtcat attgggcgac ggctttctgc ccatcagaaa tagccaggcc tgcatcttgc
    47641 gataccggcc cggcttgtta tcaacactta atgggttttg atgtagccgc gcacgcactc
    47701 ttcgactaag ccccagaacc agtcgctatc aatagtgatg ccgactttgg tattagcggg
    47761 cttgccaaga acgcccagct cgtcgcagac ggtacgccca taacaagggc cactgttgac
    47821 gtccacttcg acgtacatct cctgggtttt aatgccatca gggttaatca gataaccgat
    47881 gcaggtggcg tcgtgcaccg ggccgccagc caggccgtag ttttcgaact gggttttgag
    47941 agtgaagttc atgatgtcgc tgaacagctc tccggcaggg ccgcctgccc tttccattcg
    48001 tgcaatcacg tccggggtgc aaacggtctg gttggtgaga tcgaggccca tcatcactaa
    48061 tggaacgcca gaggtgaaca ccacgcgtgc ggcttccggg tcagcaaaga tgttgaactc
    48121 ggcagatggc gtgaagttgc ctgtaccgta agcgccgccc attagcacaa tttcacggat
    48181 tttgggcagg atcgcgggtt gcatacgcat tgccaccgcg atatttgaaa gcggaccaac
    48241 cggcaccaga gtgatatcgc catcgcttgc catcagggta tcaatgatgt atttcaccgc
    48301 atgagtgctt tctgcctggc gggtcagcgg ctcgaatacc gggccatcca gtccggtatc
    48361 accgtgaata ttatcggcaa cgatttgttg ccgcataatg ggttgtggca tccccgcata
    48421 aaccggaaca ttaatctcca gtttctggca aacattcagg ccattaatta atgttttatc
    48481 aagcgtctga ttacccgcta caatagtgat gcctaataaa tctattgccg gatgtttcgc
    48541 cgccatcatt atagcaatag catcatcatg acccggatca caatccagaa taatttttct
    48601 cttttccatt gtttatttcc tctgtttcca gttgcgttat tttttctaca gcaaagaaat
    48661 ttcgatcggg ccgatgattg aatcttaaca acagcgtacg tatgctaaat atgagaaatc
    48721 tcatagcgga taaacatcgt gaaagaaatc cacaataatg atcttaagca gcaattgatg
    48781 agtgaatccg cgtttaagga ttgcttttta acggatgttt cagccgatac gcggctgttt
    48841 cattttttag cgcgtgacta cattgtgcag gaagggcaac agccgtcctg gctgttttac
    48901 ctgacgcgag gccgcgccag gctttacgcc acgctagcta atggtcgcgt gtcgctgatc
    48961 gatttctttg ccgccccctg ttttattggt gagattgagt taatcgataa agaccatgaa
    49021 ccgcgtgcgg tacaggctat tgaagagtgt tggtgccttg cgctccctat gaaacattac
    49081 cgtccgctgt tattaaacga caccctattt ttacgaaaac tttgcgtcac cttaagtcat
    49141 aaaaattatc gtaatattgt ttctttaact cagaatcaat catttccgtt agtgaatcgc
    49201 ctggcagcat ttatattact ctcacaagaa ggtgatcttt atcacgaaaa gcatacgcaa
    49261 gcggcagagt atttaggcgt ttcttatcga catcttttat atgttctcgc gcaattcatt
    49321 cacgacggtt tattaattaa aagcaagaaa ggatatctaa ttaaaaacag aaagcagttg
    49381 tcagggctgg cactggagat ggacccggag aataaattct ccgggatgat gcagtaaaaa
    49441 ttatttgcaa tagcgcgatt gccggatgca acgcttgatg cgttttatcc ggtctacaaa
    49501 ttgagcatta cgccagacca ataaagaacc cggcaatggt cgcgctcatc aggttggaaa
    49561 gcgttgctgc tgccagcgcc cgtaaaccaa gctgagcgat ttccggtgcg cgttttggcg
    49621 aaatagccga aaatgcccca acgacaacac cgatagaacc aaagttagca aaaccacaaa
    49681 gcgcaaagga gataatcgca atggttttca cttccagcgt gccgcccgtt tgcaggtatg
    49741 gggagaaact caggtaagcg acgaattcgt taatcgccag tttctgccca atcaggctac
    49801 ccgcaaggtt ggcatcactc cagtccacac ccatgatcca cgccagcggt gccagcacat
    49861 agccaaaaat actttccaga gaagcattgg cgaagccaaa ccagccgcca attccgccga
    49921 taatgccgtt gatcagcgca ataattgcca caaatgccat taccaccgtc gccacaccag
    49981 cggcgatttt cagcccggtc atcgcaccgc tcgccgctgc ttcgataaag ctttttggcg
    50041 gcgtttcgct gaacgacaga ttttcaaacg tgacctgcga aagctcggtt gctggactaa
    50101 gaatacgtgc aaacaaaata ccgccgggga tcgccatcag cgatgccgcc aacaggtagt
    50161 caattggtac gcccattccg gcataaccaa tcatcatcga accagcaatg gacgccatcc
    50221 cgctacaaat agccgtaaac aactcgtttc gattcatgcg atcgataaac ggtttaacga
    50281 tcgccgggat ctcattttgc ccgaggaaaa tagtggtgac tgcgacaaaa gattcgattt
    50341 tgctgatgtt gagggctttc tggaaaatgc tgccaaggat gcgaatcagc agccccatca
    50401 cgccaatgta gtacagcaga ctaatgagcg cagtgacgaa gataatcgcc ggaagtacgc
    50461 gaaaggcgaa gataaaaccc gcaccgtcaa agaggacatc cattttcggc ccgaccagcg
    50521 aaccaaaaat gaaggcgcta ccagcatcac tgtaagacat cactttatga acgcctaatg
    50581 ccgcctgttc tacagcccac tttcccggcg gaaaatagag catgatgcca ccaagagcaa
    50641 tttgcagcag tagtgcggct gcaaccgtac gcaaactgat gctcttttta ttcactgaca
    50701 acagaaatgc tattgccagt aacaccacca tccccacaac acttctcatt atgtccataa
    50761 tgattttccc ttcatgccag taaacccggc gtcagcgcca ggtttcggta tgtttgatga
    50821 gtacgggcga cggctttctg cccgtcagaa attaacccgc gagacgctga tattctttgg
    50881 caatttcgct cgccagaatg gcgttgttga acacaagctg gatgttggat ttcaggctgt
    50941 caccgccggt cagctcagca acgcgcgcca gcaggaatgg cgtactttct ttaccaataa
    51001 caccctgcgc ttcagcttca gctaccgcct gatcgatcac cgcattgata gtgtgttctg
    51061 gcatcgcaaa ctgttccggg atcgggttcg ctaccacgag gccaccgttc agcccgcttt
    51121 gccatttcac cgccattgca cgggcaattt cactggcgct gtcgagacga atgctgacgt
    51181 caaacgggct ggtacggcag aaaaacgcag gcagcgcttt agtctgatag ccaattaacg
    51241 gcacaccgaa ggtttctaaa tactcagtgg ttaatcctaa atcgagaata gatttcgccc
    51301 cggcacaaac aacggtgaca ttagtatttg ccagttcttg caaatcggca gaaatatcga
    51361 aggtatgttc cgcccctcga tgaacaccac caattccgcc ggtggcaaat actttaattc
    51421 cggcaagcgc cgcaataatc atcgttgaag ccacggtggt tgcaccattt tttcctgcgg
    51481 caacgacaaa aggtaaatcg cgacgactaa ctttagtcac gttatgccct tcacgaccca
    51541 gtaattcaat ttcttctttg cttaaaccca ctttcatcac gccgccaata atggcgatag
    51601 tggcaggtac tgcgccctgt ttacgaatag tttcttctac ttcaattgcg gtctgggcat
    51661 tttgtgggaa cggcatcccg tgagaaataa tggtcgattc cagcgccaca accggttttt
    51721 tgttttttaa agcgtcctgc acttccgggg aaatttgtaa taattcaggg gaaattttta
    51781 attcagacat tctgcgttct ccactaacga tataacgttg gcaatcgata aatcggggtt
    51841 attggtgtat tcacaggaga gcgccattga cgaacatccc tgtgcgaaac gaacagattc
    51901 ggcaaacggc attccgtcta cccaacacga agcaagtccc gccatcatgg catcgcccgc
    51961 tccggtaaca ttaataacat tggttttgat cggcgcagac cagccacttt caccgctgat
    52021 atcgctgtaa taaacgccgt cgccgcccat gctcaatacc agtcggttca ggccatgttg
    52081 atggaaccag gcagcaactt ttgccacatc ttcacgccct gacagcgcaa tcccactcag
    52141 ggtttccgct tcaaggcggt ttggcttgag agtatggatt tgacttaatc gctcgcgtac
    52201 tttgacgcat ttccacgcgg aaaccggatc gacaaatacg ggtacgttgg cagcattatc
    52261 cagaatccat gccagtgcct cttcactgat attacagtcc gcgacaatga cctttgccct
    52321 ctgaataaat tcacggtgct gtgcgagata ttcagctgta atagcgttgc taatattcat
    52381 gtcatttata gcaaccagca tttcaccggt attatcgagt aatgataaat agctcgacgt
    52441 attttctccc ggcacaatca ggcatttatc gacataaacg ccagattgat tggtttgcgt
    52501 tagcagcgat tggccataaa aatcactgcc tacggcgctc agtagccagg ctttattacc
    52561 cagcaacgcc aggttttgtg caatattgcg ccctacccca ccaggcgtaa attttatttt
    52621 acctggattt gaatccgcat aatttaatga ttcatgtgaa tatccggcga catcaatatt
    52681 cgccgagcct ataattacga cataatcctt ttcgcgcata gcacgtcctc tggtaaaaaa
    52741 ttaccaaccc tgtcaatcca caaggatata gatactgcaa agacggttta aacatgtgtt
    52801 catatttaaa catgtgctca tagtatgcat gttttacgta aagtaaattg ccgccagaga
    52861 ggcaaagcgg gcagatttga gcaggctcac aaaatgcgat taaatgcctg aattacaaaa
    52921 gcgaggaatt tgtgacggga tgcacagaac atgaacacta aaaaagggca gaaaagaatc
    52981 tgcccttgag gaaataacct tagtgtttga tgaacgtttc aggtttctac tgaaagataa
    53041 aaccatttaa attcataaaa ttacattcac aaaccggtca ttctctttgc gctcatccaa
    53101 tgatacgggg cagtaaattt ctgccctgta acacaccttt tattacgcgg ctttcgctac
    53161 tgcgtccact tccggacgtt tcaggaaggc ataggccaaa cccgccacca gcgtaccggc
    53221 aataattgct accaggtaac ccagtaccgg cgtaatagcg ccagggatca gcagaacaaa
    53281 cagaccaccg tgcggtgcca tcagtttcgc accaatcgcc attgagattg cgccagtcag
    53341 cgccccacca acgatacaac acggcagcac acgcatcgga tcacgagcag caaaaggaat
    53401 tgcaccttcc gaaataaagc acagaccaag aaccagagcg gctttgccac cttcctgctg
    53461 cgctttgtca aatttgcgac gcgccaccat tgttgccaga cccattgcca gcggtggcac
    53521 catacctgcc gccataatcg ccgccatcgg gccataggtt tgagtactca gcagacccac
    53581 accgaatgcg tacgctgctt tatttaccgg accgcccatg tcagtacaca tcatgccacc
    53641 gaggatcgcc cccagcagaa ccgcattcgc agtccccatg gtctgcagcc agtgagtcaa
    53701 cccttcgaga atgccagcaa ccggtttacc gatcaggtag atcatcgcca gaccgaccac
    53761 cagactggaa attagcggaa tgatcaggat cggtttcagc gcctccatac tctgtggcag
    53821 tttcagttgc gtactgatta acttcgcaat gtaaccagcc aggaagcccg caataatacc
    53881 gccaatgaag ccagaaccgg tgctgaccgc cagcataccg ccaatcagac ccggcgtgag
    53941 acccggacga tcggcaatgg agaaggcaat ataacctgcc agtaccggca ccatcagcgc
    54001 aaaggctgaa ccaccaccaa tctgcatcag cgccgctgcc aacgtacccg gctctttaaa
    54061 cgcttcgata ccaaaagcaa aagaaagcgc gatacacaga ccacctgcaa cgaccatcgg
    54121 cagcatgtaa gagacgcccg tcagcaagtg acggtatgcg cctgcactct cgttcttacc
    54181 ttcagaggtc gccgtttgag ctttgcccgc cggttcatac ggcgttgctt cagcaaccgc
    54241 tttatccagt tcctgcgcgg ttttcttcag cgccagaccg gtagaggtgc gatacatcgg
    54301 tttaccagca aatttcgcca gatccacttc gatatctgcc gccacaatca ccagatccgc
    54361 tgcggctact tcttcgggag tgattgcatt acccgcgcca acagaaccac gggtttcaac
    54421 tttcacccac cagccacgtt ttttcgcttc ggtttcaatg gcttcagccg ccataaaggt
    54481 gtgtgctacg ccagtcgggc aagcagtcac cgcaactacg cgtttcggac cgctggcggc
    54541 aaccggtgct gtcgcagcga ccggcgcagt gtaaggtttc gcatggcctt tggcttcact
    54601 caggaacagc tcagggtgcg caactgcccg ggaaatatcg cccagccaga catttttacc
    54661 gttcagcgcg ctgtcattcg ggatggaatc accgagaaca atcgccattt ctgcgtcgtt
    54721 cggattgtcg atgatttcca gttttgcttt tcgcgccgcc gcgcccagca gggtcttcgc
    54781 catataggcg cgtgcctgac cgagattagc gtcaataatc agcagcgttt tcattatgcc
    54841 tctcctgctg tcagttaaaa ggttgtaagt cgacgcgcgc catcattgcg gccaactgcg
    54901 gacgatcggt aatacccaca ttgctttgac ttaccgccag ggctgcaaca gcagtcgcca
    54961 gacgcagtgt gtgttcactg gattcacgca ttagcaagcc ataaatcagg ccaccaacca
    55021 tagaatcccc tgcgccaacg gtgcttacga catcgactga cggtggtttg gcgatccatt
    55081 cgccggaggc attaacccaa agcgcgcctt cggcacccag tgaaataacg acatgcgcaa
    55141 taccttgttc acgcagcgca tgcgcagctt caatcacatc tttcatttca ggcagtttac
    55201 ggcctgccca gatttccagc tcgcggcggt taggtttcac cagccacggt gccgctttca
    55261 aacctgctac taacgcttca cggctactat caaagataat gcaagggcac tgactacgca
    55321 ggcgagtcat ccagtcggtg aacgcttccg ggctgacgcc tgacggtaag cttccgctga
    55381 cacagaccat atcgaactga ccgagccagc tcagagaatc agtcacaaag cgttcccagt
    55441 cggcgggggt gacttcaaaa cccgagaagt tgaagtcggt cacttcgccg tctttttccg
    55501 tcagcttaac gttaattcgg gtgcgcccct gtacaacctg gaaacggttg gcaatgccca
    55561 gctcgctgaa cagttgctga aaaccatcct gattgtcttt accgaggaag ccgccaacgg
    55621 tgacatcaat tcccaggtct tttaatactt tggccacgtt gatgccttta cccgccgcat
    55681 gcagaccggt ggttttcacc aggttcactt cgccgcgttc aatttccggg cagaaaccaa
    55741 caaggtcata agccggatta agggtgatag tagcaacacg tctgctcatt atgcgccctc
    55801 cccaagacca gcagcgatag cgtcgccgat tgctttcagc gcctgttcag catctgcacc
    55861 ctgggcggta aagcgtaggc gatgaccttt cttaacgcca agtgccacaa ctttcatcag
    55921 actacgtccg tttgccggtt tgccggtgcc atcaaggttt gtcacggtaa tatcactgtt
    55981 aaattgttta atggtgttga ccagcatggt acctggacga gcatgcaggc cgtgttcatt
    56041 gcgcaccaca aactccgcgc ttaacacgtc gtcggtcggc gcatcatcgc tggtcagcag
    56101 cgccagcaac gttgccgcat ccgctttcag caagcggtca gctttattgt cgagcaacaa
    56161 atcagcgaga cgcttaagaa ccgcaatggg ctgatcgtca ttcatcgcca cactcaccag
    56221 catggctgcc gtttcgccgt ccacatcaaa agcatttgcc gcacggctta ccgcaattgc
    56281 gctacgcaga ttgccttcgg cgctatcgct cagccagata ccctgtccga ggttcagcgg
    56341 ttgttcattg atggctttgg tgacgaaagt ggcgtcaact gcccccgcct ctttcagacg
    56401 cgcagcgttc agcgcctgaa gagtcagcag atcgctggcg acgatatcca gcgtcagcat
    56461 ttcgttgtcg agcttcagct gctcactctg cttttcgccc atcagcaatg cgcgaagttc
    56521 ttctgctgtt gttgctgact tcagttgttc agcaacggaa tcatcgctca gtacgtgggt
    56581 cagctggcgt agcaggccca gatgctcatc cgagctggca gcaataccga tcgctacgta
    56641 cgctacctga ccgtcacccc aggtgacgcc ttccgggaac tgaaatacct gaacgccggt
    56701 tttcagcacc tgatcgcggg tgtcggtagt gccgtgtgga atagcaatac cattgccgag
    56761 gaacgttgag gtttgctgtt cgcgcgccag catgccattg acgtagcctt ctgctacatt
    56821 accggcctgc accagcgccg cagcgacctg gcgaatcgcc tcttctttgt ctccggcctt
    56881 ttcgcccgga tggatgtcct gtacggataa ctggaacata gttctcctct cttgctgaat
    56941 tgaaacgatt cagcttctat gagaaaaaaa gcgccaacct ggcttagggt taaagacaag
    57001 atcgcgctga aacgtttcaa gaaagcataa tacttctgtt tcagcacgca aggaaagccg
    57061 taaagttatg agcaaaagtt tgatgtgctg cacaattttt tgtcattttt cctaattgct
    57121 gatgggaaaa tccgttatca gcaatttcat ttcagcagcg tcagcagcca atcagcaaca
    57181 gtgaaatggc attttgatta ttcgtggcgc ttttgatttc gctgtctgtt tattttctga
    57241 caagcagcgt aaactccgcg tcttcctctt ccagtgatcg accagcatgc ataactcccc
    57301 cgcagtctcc agcgcgaaat cgtttgacct gacctcgacg gcgtttttaa tcgttgcctt
    57361 tctcaccggt attgcgggcg ctctgcaaac cccgacactc agtatttttc ttaccgatga
    57421 agtacatgcc cgtccggcga tggtgggatt cttctttacc ggcagcgctg tcattggggt
    57481 tctggtgagt cagtttctcg ccgggcgctc tgataagcgc ggcgatcgca aatcgctgat
    57541 tgtcttttgc tgcctgttag gcgtgctggc ctgcaccctt tttgcctgga atcgcaacta
    57601 ctttgttttg ctattcgttg gcgtctttct tagcagcttt ggctcgaccg ctaacccgca
    57661 aatgtttgcc cttgcccgtg aacatgccga caaaaccgga cgtgaggcgg tgatgttcag
    57721 ctctttttta cgcgctcagg tttcactggc atgggtcatt ggcccaccgc tggcttatgc
    57781 cttagcgatg ggtttcagct ttacggtaat gtatctgagc gcagcggtag catttattgt
    57841 ttgcggcgtg atggtgtggc tgtttttacc gtcgatgcaa aaagagcttc cgctggcgac
    57901 cggcacggtt gaagcgccgc gccgtaaccg tcgcgatacg ctgctgctgt ttgtcatttg
    57961 tacattgatg tggggctcga acagcctgta catcatcaac atgccgctat ttattatcaa
    58021 cgaactccat cttcccgaga aactggccgg tgtgatgatg gggaccgccg ccgggctgga
    58081 aatcccgacc atgttgattg ccggatattt cgccaaacgt ctgggtaagc gtttcttaat
    58141 gcgcgttgct gccgtgggtg gcgtctgttt ttacgcagga atgctgatgg cgcattctcc
    58201 tgtcattctg ttgggcttgc agctgctaaa tgctattttt attggcattc tgggcggtat
    58261 cgggatgctc tattttcagg atctgatgcc cggtcaggca ggttcagcca ccacgctcta
    58321 taccaacacg tcgcgcgtgg gctggatcat cgcgggatca gtggcgggca tcgtcgccga
    58381 gatctggaat tatcacgctg tgttctggtt tgcgatggtg atgattatcg ccactctgtt
    58441 ttgcttactg cggattaaag atgtttaagg cgcggtcagc atttccagat cgataagcca
    58501 ggtcattgcc tgctgacgcg agttaccgca catttcagcg ctggcttgta agcctgcaca
    58561 gactttcggc cgcagtggcg aggtgaaaat tttacagcgt tgctgttcat caagctgaat
    58621 gcagggcgta ttggcgggct tgccatctgg cataccggga atagggctgg aaattgaagg
    58681 ggcagtgcaa cacgccccgc aacccggacg gcattccata cgttgttctc ttaaggttaa
    58741 ccaccggatt gatgagcgcg cacagtacca ccttttttgc accagcaaaa gtgcgaatac
    58801 cacttgcccg aaaggctcgt cgcgagtact ttgtcgcgat atttttgaca ttttcgacta
    58861 caggaatttt tcgatgccaa gagcgaacga aattaaaaaa ggtatggtac tgaattacaa
    58921 cggcaaactg ctgctggtta aggacattga tattcagtcg cccactgccc gcggtgccgc
    58981 tacgctgtac aaaatgcgtt tttctgatgt ccgtaccggg ctgaaagtag aagagcgttt
    59041 caaaggagat gatatcgttg acaccgtgac gctgacccgc cgttacgttg acttctccta
    59101 tgtcgatggc aacgaatatg tctttatgga taaagaagac tataccccgt ataccttcac
    59161 caaagatcag attgaagaag agttgctgtt tatgccagag ggcggcatgc cggacatgca
    59221 ggtgctgacc tgggacggtc aactgctggc gcttgagctg ccgcagaccg ttgatctgga
    59281 aatcgttgaa accgcaccag gcatcaaagg ggcatctgcc agcgcccgta acaaaccggc
    59341 gacattgagc actggtctgg tgattcaggt accagaatac ttaagcccgg gcgaaaaaat
    59401 tcgtatccat atcgaagaac gccgttatat ggggcgtgct gactaacttc agccgcatgc
    59461 agaaaaggga tagctcaggc tgtccctttt ttaatttatt ataccaatct tctattttgc
    59521 gcttcctgaa caccttacgc cctcaatttt cactcgttga ttgatcgccc tcacacttca
    59581 tcgcattaac aatccagacc aatttcaatt gctgtcatat aactttacac tgtcattgtt
    59641 aattaatcgt tactaagaca tgactcctat gaatacaatc gcctccgtta cgctcccgca
    59701 tcatgtacac gctccacgct acgatcgcca gcagttgcaa tcacgtatcg ttcattttgg
    59761 ctttggagcc tttcaccgcg cacatcaggc gttactgacc gatcgtgtgc tgaatgccca
    59821 gggcggcgac tgggggatct gtgaaatcag cttgttcagc ggtgatcaac tgatgagcca
    59881 gctccgcgca cagaaccatt tatataccgt gctggagaaa ggggcggacg gcaatcaggc
    59941 gataattgtc ggtgccgttc acgaatgcct gaatgcaaaa ctggattcct tagcggcaat
    60001 tattgagaaa ttttgcgagc cacaggtggc aattgtttcc ctgacgatta ccgaaaaagg
    60061 ctattgtatt gacccggcca ccggtgcact cgacaccagc aatccgcgga ttattcacga
    60121 tctacaaacc cctgaagaac ctcactccgc accgggtatt ctcgtcgaag cactgaaacg
    60181 ccgccgtgag cgcggcctta caccgtttac cgtgctctcc tgcgacaata ttcccgacaa
    60241 tggccatgtg gtgaaaaacg cggtgctggg aatggcagaa aaacgttcgc cagaactcgc
    60301 cgggtggata aaagaacacg tcagttttcc gggaaccatg gtcgaccgca ttgttccggc
    60361 tgcaaccgac gaatcactgg cagaaatcag ccagcatctg ggggtaaatg atccctgcgc
    60421 gattagctgc gaaccgttta tccagtgggt ggtggaagat aacttcgtcg ctgggcgtcc
    60481 tgcctgggaa gtcgcaggtg tacaaatggt gaatgatgtc ctgccatggg aagagatgaa
    60541 actgcggatg cttaatggca gccactcttt tctcgcttat ctgggttacc tctcaggatt
    60601 cgcccatatc agtgattgca tgcaggatcg cgcatttcgc catgccgcca gaacattaat
    60661 gctggatgag caagcgccga cactgcggat taaagatgtc gatttaacac aatatgcgga
    60721 taagttaatt gcacgttttg ctaatccggc gctgaaacat aagacctggc aaatcgcgat
    60781 ggatggcagc cagaaattac cgcaacgcat gctggcaggt attcgcatac atctggggcg
    60841 cgaaacggac tggtcgttgc tggcattagg cgttgcaggc tggatgcgtt acgtcagcgg
    60901 cgttgatgat gccggaaatg ccattgatgt tcgcgatccg cttagcgata aaattcgcga
    60961 tcttgttgcg ggcagcatca gtgagcaacg cgtcaccgcc ctgctttccc tgcgtgaagt
    61021 tttcggtgat gatctgccag ataacccgca ttttgtgcag gccatcgaac aagcccggca
    61081 acaaatcgca caattcggcg cgcatcaggc gctattaaac accctcaaaa tttaacgatt
    61141 tctgcggtta aagcggatga agctcgcctt cgtccgctct ccccttctct tttctgccgt
    61201 ttttagccag gattaacgct cagttaactt accagagtat tgcggagctg ttgtgaccag
    61261 gaccaacctc atcaccggtt ttctcggcag cgggaaaacc acgtcgattc ttcatctgtt
    61321 agcccataaa gatcccaacg aaaaatgggc ggtactggtt aatgaatttg gggaagtcgg
    61381 aattgatggt gctttgctcg ccgatagcgg cgcactgctg aaagagatcc ccggcggctg
    61441 catgtgctgc gttaatggtt tacccatgca ggtagggttg aataccttac tgcgtcaggg
    61501 aaaaccagac cgcttgttga tagagccgac tgggctgggc catccgaaac agatcctcga
    61561 tctgttaacc gcaccagtct atgaaccgtg gatagatctg cgcgccacgt tgtgcattct
    61621 cgatccacgc ctgctgctgg acgaaaaaag cgccagcaat gaaaacttcc gtgaccagct
    61681 ggctgccgca gacatcattg tcgccaataa atccgaccgt gcgacacctg aaagcgagca
    61741 agcgctacag cgctggtggc agcaaaatgg tggcgatcgg caattaattc acagtgagca
    61801 tgggaaagtt gccggtcatc ttctggattt gccgcgtcac aatttagccg agttgcccgc
    61861 cagcgccgcg cattctcatc agcatgtcgt gaaaaaaggg ttagcagcgt taagcctgcc
    61921 agagcatcaa cgctggcgtc gcagtctgaa cagtggacaa ggacatcagg cctgcggctg
    61981 gatattcgac gctgatacgg tattcgacac cattggcatt ctggaatggg cgcgacttgc
    62041 accggtggaa cgcgtcaaag gcgtgctgcg tattcccgaa gggctggtgc gaatcaaccg
    62101 tcagggcgat gacctgcaca ttgaaacgca aaacgttgcg ccaccggaca gccgtattga
    62161 gctgatttcc agcagcgaag ctgactggaa tgccttgcag agcgcgctgt tgaagcttcg
    62221 tttagcgact accgcgtaag gttgcctgcg tttttcagta agataattag agaaaatatg
    62281 attaaaaatt tgccgcaaat agtgttgttg aatattgtcg gcctcgcgct gtttctttcc
    62341 tggtatattc ccgttaatca tggattctgg ttgccgattg atgcggatat tttttatttc
    62401 tttaatcaga aactggtcga aagtaaggcc tttttgtggc tggttgcatt gaccaacaat
    62461 cgcgctttcg acggttgttc actgctggca atgggaatgt tgatgctgag tttctggctg
    62521 aaagaaaacg ccccaggcag acgacgtatc gtgattattg gtctggtcat gctattaact
    62581 gcagtggtat taaaccagct gggtcaggca ttaattcctg taaaacgggc cagcccaaca
    62641 ttgactttta ccgatattaa ccgcgtcagc gaactgctct ctgtccccac gaaagatgcc
    62701 tcacgagata gctttcccgg cgatcacggc atgatgctgc ttattttttc ggcattcatg
    62761 tggcgttatt tcggcaaagt tgcaggtctt atcgccctta ttatttttgt ggtttttgcg
    62821 tttcccagag taatgattgg cgcacactgg tttactgaca tcattgtcgg ttcgatgacc
    62881 gtgatattga tcggtttgcc ctgggtgttg ctgacgccat taagtgatcg attaatcacc
    62941 ttttttgaca aatcactacc gggaaaaaac aaacatttcc aaaacaaata actcacagta
    63001 cttaacatca tcaggtttat ttttatagtg aggataatcc tgatgatgcg caccgtgctt
    63061 tcatccatcg aaagcaaaaa tcattctcta agtaaatgaa tggattgcat gcatttcact
    63121 caatcgtact ttaattgacc aatcccgctt attaactttc tgtatcactt tttcttataa
    63181 aaaatcatgt aaaaccgctc gccaagaccg caccaatcgg gtaatctcga actcgttttg
    63241 cctcggcggt agattatcct cacagcatat aattttgtgc gttagtccac agatttggcc
    63301 ttaaggaatt gtttcaacat gccctggtaa ttagtctcgt gtcgcttggc atttttttta
    63361 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
    63421 gattttgaga tatatcttgc gcgggatccc cgcgattgca gtagcggttc tgctttctgc
    63481 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga
    63541 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
    63601 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
    63661 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
    63721 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
    63781 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
    63841 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
    63901 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
    63961 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
    64021 cactgtttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
    64081 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
    64141 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
    64201 tgttgcaggc gtaatgattg cgatactggt gagctgcctt cagtttttag tggcctggca
    64261 taagcacgaa gtcaaatacg acacactgat taccgacgta caaaagtatc tcgataccta
    64321 ttttgccgac ctgaaatcca ctactgaccg gctccagccg ctgaccttag atacatgcaa
    64381 gcaagctaac cccgaactga ccgcccgcgc ggcgtttagc atgaatgtcc gaacgtttgt
    64441 gctggtgaaa gataaaaaaa cattctgttc atctgcgacc ggtgagatgg acattccact
    64501 caatgaattg attcctgcgc tcgacattaa taaaaatgtc gatatggcga tcttacccgg
    64561 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
    64621 aaatagcggc gtctttgccg ctctgaatct caacctgacg ccttctctct tttatagctc
    64681 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
    64741 ttcttcacgt ttgatgaacg ttaacgaatt aaccgacatg ccagtccgtg aaactaaaat
    64801 tgcgggcatt cctctgaccg ttcggcttta tgcagatgac tggacatgga acgatgtgtg
    64861 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgctct gctattacct
    64921 gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt
    64981 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgagtaacgg gcctggaagt
    65041 actgctacgc tggcggcatc ctgtcgcggg agaaattccc ccggatgcct tcattaactt
    65101 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
    65161 cgatgccgca gaattagaaa aagtgctgcc ggtaggcgtc aaatttggca ttaacattgc
    65221 gccggaccat ctgcacagcg aaagctttaa agcaaatatc cagaaactgc tcacttccct
    65281 acccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
    65341 agaagccaca caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga
    65401 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
    65461 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
    65521 cgcggtgctg acgctggcga aacgcctcaa tatgctgacg gttgctgagg gggtcgaaac
    65581 gccggaacag gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
    65641 tagtcgcccg ttaccgctgg acgattttgt ccgctggctg aagaaaccgt atacgccgca
    65701 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
    65761 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
    65821 tctgttcacc tttggtgcgc aggcgcaggc tatcaaggaa agctatgctt ttgccgtgct
    65881 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
    65941 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
    66001 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgctat ttacgacttc
    66061 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agcgcacgct atgctgacga
    66121 ttattcctgg gtggaggtcg ctattaatcc tcgcgcccgt tttcatgatg gttcgcccat
    66181 tactgcccgc gatgtagagt ttacttttca aaaatttatg actgaaggcg tgccgcaatt
    66241 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
    66301 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
    66361 aaagtactgg aaggatcaca aacttagcga tccgctcgcc acgcctccgc ttgccagtgg
    66421 tccgtaccga attacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
    66481 ttactgggca gcaaacttac cggtaaaccg tggacgctgg aatttcgaca ccattcgcta
    66541 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
    66601 gcgtatggaa aacgacgcca aaaactgggc cacacgctat agcggtaaaa atttcgataa
    66661 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
    66721 gtttaatatc caacgtccgg tattcagcga tcgccgggtc cgggaagcaa tcaccctcgc
    66781 ctttgacttt gaatggatga acaaggcgtt gttttacaat gcctggagtc gcacgaacag
    66841 ttattttcag aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
    66901 tctggcacca atgaaaaaag atttaccgcc agaagtcttc acacaaatct accagccgcc
    66961 ggtatcgaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
    67021 cgaagcgggc tgggtgctga aaggtcagca acgcgttaat gccactacgg gtcagccgct
    67081 cagctttgaa ttattgcttc ctgcaagcag caatagtcag tgggtattgc cgttccagca
    67141 cagcctgcaa cggctgggta tcaacatgga cattcgcaag gtggatcact cgcaaatcac
    67201 caaccgcatg cgcagtcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg
    67261 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
    67321 ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg
    67381 aaataaagaa aaattactgc cgttagggcg agcactggat cgcgtattaa cgtggaatta
    67441 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
    67501 ccaacccgct gtgcgccctg tttacagtct gggtatcgat acctggtggt atgatgttaa
    67561 taaagcggcc aaactgccgt cagccagaca acagggagag tagatgggcg cttatctgat
    67621 tcgccgtctg ttgctggtga tcccaacgtt atgggcgatt atcactatca actttttcat
    67681 cgtgcaaatt gcgcctggcg gtccggtcga ccaggccatc gccgccattg agtttggtaa
    67741 tgccggagta ttacccggcg caggcggtga aggcgttcgc gccagccatg cgcaaacggg
    67801 cgtcggtaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
    67861 cgccgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
    67921 gctctgggac tacatccgct ttgattttgg cgatagcctg tttcgcagcg cctcggtgct
    67981 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
    68041 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagccgctt
    68101 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
    68161 catcctgctg attgtcttct tcgcaggcgg cagctatttc gacctgttcc ctctacgcgg
    68221 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
    68281 gcatatcacg ctgccggtgc tggcgacggt gattggtggc tttgcggcgc tgactatgct
    68341 gacaaaaaac tcattccttg atgaagtacg caagcaatac gtggtgaccg cgcgtgcgaa
    68401 aggggtaagt gaaaaaaata ttctctggaa acatgtgttc cgcaacgcca tgctgctggt
    68461 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    68521 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    68581 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    68641 tgtcagtgat atcagctata cgctggtcga tccgcgtata gattttgagg gacgttaatg
    68701 tcgcgactca accccgtcaa tcaggcccgt tgggcgcgtt ttcgccataa ccgtcgcggc
    68761 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    68821 gccaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    68881 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    68941 tggctgaaac aacggctgga aaataatggc tgggtactgt gggcaccgat tcgctttggt
    69001 gctaccagta tcaactttgc taccgataag cccttccctt ctcccccctc ccggcaaaac
    69061 tggctgggaa cggatgccaa cggcggcgat gtgctggcac gtattctcta tggcacgcgg
    69121 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    69181 ggggcgctac aaggctatta cggcggtaag gtcgatctct gggggcagcg ctttattgaa
    69241 gtatggtcgg ggatgccaac gctgtttttg attattctgc tttccagcgt tgtgcaacct
    69301 aacttctggt ggctgctggc aattactgtc ttatttggct ggatgagtct ggtcggcgtg
    69361 gtgcgggcgg agtttttacg tacccgcaat ttcgactaca tccgcgcggc acaggcgctt
    69421 ggcgtcagcg atcgcagtat tattctgcgt catatgttgc cgaatgcaat ggtcgctacc
    69481 ctcacctttt taccgtttat tttatgtagt tcgatcacca ccctgacctc actcgatttc
    69541 ctcggcttcg gtctgccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    69601 aataaccttc aggctccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    69661 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    69721 tagcatgacg caaactctgt tagcgattga agatttgtcg gtgggttttc gccatcagca
    69781 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    69841 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    69901 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    69961 tcatgccagc gaccaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    70021 agagccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    70081 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    70141 tcgcgttggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    70201 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    70261 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    70321 gcgcgaactg caaggcgagt tgaatatggg catgctgttt attactcata acctcagcat
    70381 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    70441 ttacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    70501 tgaaccgtca ggcgatccag tgccgttgcc agaacctgcc tcaacgttgc tggatgttga
    70561 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    70621 tgtggtggtg aaaaacatca gttttacgct acgagcgggt gaaacactgg gtttagtggg
    70681 cgagtccggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttaattctca
    70741 gggcagcatc atctttgacg gtcagccact gcaaaattta aatcgccgcc agctgttacc
    70801 tattcgtcat cgcattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    70861 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    70921 acaacgcgaa caacaagtga tagacgtgat gcatgaagtg ggattagatc ctgaaacacg
    70981 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    71041 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    71101 ggtacaggcg caaatattga cgctattgaa atcattgcaa caaaagcatc aactggccta
    71161 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    71221 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    71281 ggagtatacg cgtcagctac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    71341 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    71401 ggcgatttac aaacaggcac ggctcacctt cacactccag caccgaacat ggaacttcga
    71461 tgtcgcctaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    71521 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    71581 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    71641 ttgagagttg taatgaagtc acttattttc cccggaagca catttacttc acaatttgtt
    71701 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    71761 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    71821 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    71881 actttttaca taaattgatt ttacataaaa taaacatata tcgggggaat gatagatttg
    71941 tggttgacat gcatcaacaa aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg
    72001 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    72061 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    72121 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    72181 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    72241 cgccgcaacg ccaaccacca gcgaccaaaa tcccagaccc agcagcgcac tgatgaccat
    72301 ccacgctgcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    72361 acggcggacg aagcggctat taaagatggt catcacgaac aggaaaacaa tgtttagtgc
    72421 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatgtaga caaacggtcc
    72481 ggcgcttaag aacgagaaca tcccggcaaa gctgaaacca ctggccagca tataactcag
    72541 gacacgctta tgacggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg
    72601 ctggcgacgc tccggtggta aggtttcttt gatcagaaag aagatcattg ccgaagccag
    72661 aatcgccgcc acggcgagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    72721 gccgccaact atcggtgcca tcagcggtgc aatggtcgtc accagcatga caaacgacat
    72781 catccgcgag aactcttctt tcgggtagat atcacgcatc aaagcgttga taaccacgct
    72841 ggccgcagcc gccgccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    72901 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtaccgccaa gaaccaccgg
    72961 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    73021 aatataagta ctgagggtca tctgcgtact gcccgccggt acgccaaact gcgctgaaat
    73081 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    73141 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    73201 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    73261 gggggctaaa tcagcatcca gcgtaatgcc gccaatacgt tcacgatgca gctcaaccac
    73321 atggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    73381 cagacgaacc tgcgttgggg taatcacttc aagcaccgct ggcttagtga gatctttttc
    73441 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta cgggtgattc
    73501 cagcgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    73561 ctgaccatca tctgtcatta gtaccagacc ggtggtatcg atatccagcc gccctgcggc
    73621 gtgcagtttc cacgccactg gttcatctag aaaatagagc accgttgggt gatcagggtc
    73681 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    73741 ctgcgccagc gggttgccat cgtaagcgac atcatgttca ggaagcagtt tgaacgctgc
    73801 attacggacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    73861 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    73921 gcctgtaaaa acggaggtcg ggcattgacc cgaaaatctg aactgttgtc tgcccagtat
    73981 agcggtctaa taacttccct caagggaaaa agaaccgtgg catactatta gcagaataat
    74041 ctacctacgc gagaccatga tttttacact ccgcccatat cagcaagaag ccgtggatgc
    74101 cacgctcaac cattttcgtc gtcataaaac ccctgccgtt atcgtgctgc ccaccggtgc
    74161 aggtaaaagc ctggtgatag cggaacttgc gcggctggct cgtggtcgcg tgctggtgct
    74221 ggcacacgtt aaagaactgg tggcgcaaaa ccatgcaaag tatcaggcgc tggggctgga
    74281 agccgatatt tttgccgccg ggctaaagcg caaagagagc cacggtaaag tggtatttgg
    74341 cagcgtgcag tcggtcgccc gtaatcttga tgcctttcag ggtgaatttt cgctgttgat
    74401 tgtcgatgaa tgtcaccgta ttggtgacga tgaagagagc cagtatcagc aaatcctcac
    74461 tcacctgaca aaagtgaatc cccacttacg cctgctgggg ctgactgcca cgccttttcg
    74521 actgggcaaa ggctggatct accagttcca ttatcacggc atggtacgcg gcgatgagaa
    74581 agcccttttc cgtgactgca tttatgaact gccgctgcgt tatatgatta aacacggcta
    74641 tctgacgccg ccagaacgac tggatatgcc agtagtgcaa tacgatttca gccgcttgca
    74701 ggcacagagt aacgggctat tcagcgaagc cgatctcaac cgcgagctaa aaaaacaaca
    74761 acgtattacc ccgcacatca tcagccagat tatggagttt gctgcaacgc gcaaaggggt
    74821 gatgattttc gccgccaccg ttgaacacgc aaaagagatt gtgggattac tacccgccga
    74881 agatgctgca ctgattactg gcgacacccc cggcgctgag cgcgatgtgt taattgaaga
    74941 ttttaaagcc cagcgttttc gctatctggt caacgtcgcg gtactgacca ccggatttga
    75001 cgccccgcac gtcgatctta tcgccattct gcgccctacc gaatcagtga gtctttacca
    75061 acaaattgtc gggcgcggtc tgcgtctcgc tcctggcaag actgattgct taattcttga
    75121 ttatgcgggt aatcctcacg atctctacgc gccggaagtt ggtacaccga aaggcaaaag
    75181 tgacaacgtt ccggtacagg ttttctgccc tgcctgcggt tttgccaaca ccttttgggg
    75241 gaaaacgacc gccgacggga cattgattga acactttggt cgccgctgtc agggatggtt
    75301 tgaagatgac gacggtcatc gcgaacagtg tgacttccgt ttccgtttta aaaattgccc
    75361 gcaatgtaac gcagaaaatg atattgccgc tcgccgctgc cgcgaatgtg acacggtact
    75421 ggttgatccg gatgatatgt taaaagcggc gctacgactg aaagatgcgc tggtattacg
    75481 ctgtagcggc atgtctttgc agcatgggca cgacgagaaa ggcgaatggt tgaaaatcac
    75541 ctattacgat gaagacggcg cggatgtgag tgagcgtttc cgtctgcaaa cgcccgccca
    75601 gcgaactgcc ttcgagcagc tttttatccg cccgcatacg cgcacaccgg gcatccctct
    75661 gcgctggatc accgccgccg atatcctcgc ccagcaagcc ttattgcgac acccggattt
    75721 tgtcgttgcc cgcatgaaag gtcagtactg gcaagtgcgt gaaaaagtgt tcgattacga
    75781 aggtcgtttt cgtcgggcgc acgaattacg cggttaatgg tagttttgat tgatgtataa
    75841 ggcgattgag tatagaatct cgcccgcttt tgcatacgca aagcagatca cttacctgtt
    75901 gctgggtcgc ctgtagcagg attaatttaa gagagaaaga aatgtttact atcaacgcag
    75961 aagtacgtaa agagcagggt aagggtgcga gccgccgcct gcgtgccgct aacaagttcc
    76021 cggcaatcat ctacggtggc aaagaagcac cgctggctat cgagctggat cacgacaaag
    76081 tcatgaacat tcaagctaaa gctgaattct acagcgaagt tctgaccatc gttgttgacg
    76141 gtaaagaaat caaagttaaa gctcaggacg tacagcgtca cccgtacaaa ccgaagctgc
    76201 agcacatcga cttcgttcgc gcttaattgc tgaataagtt gtaaaaaacc ccgctccggc
    76261 ggggtttttt gtatctgcag attatgcctg attacgatat tgctattttt tgcaggccag
    76321 ataaggcgtt cacgccgcat ctggcattca taacacaaaa ccgattaatt gccgccggac
    76381 gtccggcgct gcaattgatc gcgcaaattc ggcggcgtgc ctttaatagt cagggtatcg
    76441 gtcgccggat cccagaaaat ccgttcgccc aacagcatcg catcaaaatt aatagtcaag
    76501 ccaccgccgc taccggcaaa cttcgtcaac tgacgcagtg tgctacggtc tgccgggaag
    76561 ctctcttcca gttcgtagcc tttttcagcc gcaaactccg tgaagctcac ttcgctgacg
    76621 cctgccagct ctttcgacag cgattccagt tcgatctctt cgcctgcctg cagctgctca
    76681 ttacagtagc tataaacctg ctggcgcacg ttctgtcgct ctgctttatc cagttgtgct
    76741 tcggcagtga aatcatcaac cgcttgcaac agcccacggt tttgcgcttt cgcgttcaat
    76801 ccttcgctgg ccccgaggaa atccataaag aaatccgcca ctttgcgccc tactcgccct
    76861 ttcaggaaag tgagatagcg ggtcgactct ggattggttt cccattcggt taaatcgata
    76921 cgcgcaacaa tatccgcatg attgatatca agataatggg ttgggttgat gtcgagattt
    76981 tcgttaacgc gcatactgct caggttgctc aggactgcca ccagcagata ctccaccgcc
    77041 agataacggt agtggcaaaa cagcacgaat ccaccgtcag caaaaggata tttcgctaat
    77101 tcgtcacgca ggcgaccggt tgccgcgcgg ctaaatgcca gaaagtcctc ttcaccctga
    77161 cgctgtaagc gcagcgtctg cgccagttca ctctcttcgc taaacaagcc gtaagcttta
    77221 tttttggcgc tatagacccg atgcagttct gctaccatct caacgacggt ttctgtcggt
    77281 tccagcaatg aatcgcgcaa caccagctca aggttctgct catcacgctt gataagctgg
    77341 tgcagagcaa tctggttgat atccagactc atgataaact ctcctttaag accgggcggt
    77401 attcaaccac cgccggttaa gcgacgcaat aaaagatggc ggtcatttta cagaaggcgg
    77461 cgtaacgaga atgatttaac gcataaaaaa gcagaaaaaa aaccgttgct acggtaatat
    77521 gttgcccttt catcaactaa ctgatttcga tttatgccac aaatttcccg ctacagtgat
    77581 gaacaggttg aacaattgct cgcagagctg ctcaacgtac tggaaaaaca taaggctccg
    77641 accgaccttt ccctgatggt gttaggcaac atggtcacta accttatcaa caccagcatt
    77701 gccccggccc aacgccaggc aattgccaac tcttttgccc gcgccttaca gtcctctatc
    77761 aacgaagaca aagcgcacta agggaaacag ataacaggtt atggtaactc atcgtcagcg
    77821 ctaccgtgaa aaagtctccc agatggtcag ttgggggcac tggtttgcac tgttcaatat
    77881 tctgctttcg ctcgtcattg gcagccgtta cctgtttatc gccgactggc cgaccacgct
    77941 tgctggtcgc atttattcct acgtaagcat tatcggccat ttcagcttcc tggtgttcgc
    78001 cacctacttg ctgattctct tcccgctgac ctttatcgtc ggctcccaga ggctgatgag
    78061 gtttttgtcc gtcattctgg caacggcggg aatgacgcta ttactgatcg atagcgaagt
    78121 ctttactcgt ttccatctcc atcttaatcc catcgtctgg caactggtta ttaacccaga
    78181 cgaaaatgag atggcgcgcg actggcagct gatgttcatc agcgtgccgg ttattttatt
    78241 gcttgaactg gtgtttgcga cgtggagctg gcaaaagctg cgcagcctga cgcgtcgtcg
    78301 gcgcttcgcg cgtccgctgg ccgcattctt atttatcgcc tttatcgcct cgcatgtggt
    78361 gtatatctgg gccgatgcca acttctatcg ccctatcacc atgcagcgcg ctaacctgcc
    78421 gctttcgtac ccgatgacgg cgcgacgttt tcttgagaag catggcctgc ttgatgcgca
    78481 ggagtatcaa cgccgtctta ttgagcaagg taatccagac gccgtttccg ttcagtatcc
    78541 gttaagcgaa ctgcgctatc gcgatatggg caccgggcag aatgtcttgt tgattactgt
    78601 cgatggcctg aactactcac gcttcgagaa gcagatgcct gcgctggcag gttttgctga
    78661 gcaaaatatt tcgttcacgc gccatatgag ctccggcaac actacagaca acggcatctt
    78721 tggcctgttc tatggcatct cgccgagcta tatggacggc attctgtcga cccgtacgcc
    78781 tgcggcatta attactgcgc ttaatcagca aggctatcag ctggggttat tctcatcaga
    78841 tggctttacc agcccgctgt atcgccaggc attgttgtca gatttctcga tgccgagcgt
    78901 acgcacccaa tccgacgagc agaccgccac gcagtggatc aactggctgg gacgctacgc
    78961 acaagaagat aaccgctggt tctcgtgggt ctctttcaat ggtactaaca ttgacgacag
    79021 caatcagcag gcatttgcac ggaaatatag ccgggcggca ggcaatgtcg atgaccagat
    79081 caaccgcgtg ctcaatgcac tgcgtgattc tggcaaactg gacaatacgg tggtgattat
    79141 cactgccggt cggggtattc cactgagcga agaggaagaa acctttgact ggtcccacgg
    79201 tcatctgcag gtgccattag tgattcactg gccaggcacg ccggcgcagc gtattaatgc
    79261 gctgactgat cataccgatc tgatgacgac gctgatgcaa cgcctgctac atgtcagcac
    79321 acctgccagc gaatattcgc aaggtcagga tttgttcaac cctcaacgcc gtcattactg
    79381 ggttaccgca gcggataacg atacgctggc aattaccacc ccgaaaaaga cgctggtgct
    79441 gaacaataac ggtaaatacc gcacttacaa cttacgtggt gaaagagtga aagatgaaaa
    79501 accacagtta agtttgttat tgcaagtact gacagacgag aagcgtttta tcgctaactg
    79561 attaattata aatcagttag cgaaatatct tacttgcaat cggtgtggaa aacggtagta
    79621 ttaacaacca cgagtcggca cgtagcgcag cctggtagcg caccgtcatg gggtgtcggg
    79681 ggtcggaggt tcaaatcctc tcgtgccgac caaaaatccc aaaaaaacca accgtaaagg
    79741 ttggtttttt tatatctgca tttaattcga taaacagacc gcgacacatc agggcctgtt
    79801 tattgtctgt tatcagaacg tccagaccac acccgcctgg gtattccacg gcgattctac
    79861 ccctgcgcca ttgccatagc tgactgacaa atgaccgctt aacgtcgggg taaacgatga
    79921 ccttatccca gcctgataaa cgccacgggt tccagccaca tcattattaa atttgccatc
    79981 gtcattaact ttcacctggt tagaatcggc gtattcctga cgcacggcgg ctttcagcca
    80041 gggttccagc gtcgtaccgt tttgcaggtc catgtgatag cttaccgccg ttcccgcttc
    80101 agcgcgtaat atccgggtat ttcccacatc cgcgcgcatg ccgtttgata acgtgtagtc
    80161 ctgaccatct gtggtaaagc cggtaaaggc cagatagggt ctaacactcc acaatccgtc
    80221 aacccaacgg aacccgctct cgacatgagc acccgcgccg ttactattgt aatcgccaaa
    80281 cgctgttgcc ccattactca tcttgccatg gatggtgttg gcaaaacggt caactttcac
    80341 cactccatca acataggcac cgttctgatg ctcccaaccg gcataagccc ccagggtata
    80401 gctatcgaca ttgcctttgc cgccgcgatc aaaaccaata tcagaatgag agtaaccaaa
    80461 gaacaagccg cgaattgtgc tgctttcttc acgggagaaa cggctatcga taccgagcgt
    80521 caggcccgtc aatgtttgct caaaaccagc tcccgcatca gtggtcacgt tgttgcgggt
    80581 gttaattgcc gaactccaca tcgccgtatc gtaactaacg ccttttacgc taccaagacg
    80641 ctcacgcacg gtgtccagtt ctgcatcaaa taccagcggt tgtgcggccg ccatattcag
    80701 cacatcagtg gttgaagggg taatttgcgc gcgattctct gccagactcc agctatggtt
    80761 accattatcc agcaaggtat attcatacgt accgatatca acaacgcctc cggcattgcc
    80821 caacgtaaat gcagcatcac cgccgcccgt tgttaccagt gtaaggctat ctcctgctgc
    80881 cgggctggca ccggtgtccg tcacgaatat tttgaaatca cctgttgcct gaccggtgac
    80941 gttgagctga tcgctctgat gattcgccat atcggtacgc atataaaaat tgccgtttcc
    81001 cgacagggta ttggtggtca acgtagtgaa attttccgca cttgccgtca cagatgaacg
    81061 agtcactgac tcatccatca taatcacatt tgcgccagac atcgcaaggt tggctaagtt
    81121 aaggcgggtg ttttggttcg tcgtagtgat atcggcaatg atggtccatg cgctattttc
    81181 gagcgaaaca tccgctttgc tggtatccac ggcaccatgc gttcttaaag aagcgccctc
    81241 agaaaccact accttaccca ccaacattgc gggtgcataa tttatttgcg gcttcataac
    81301 ctcaagaatg ccatcatttc ctctgactga cacgttaacc attgtgccag cccagacgtt
    81361 agcgcggcca ttgttgataa ccatattttc agagatggct ttagaggaat acgttatact
    81421 tccattctga tctgatcgac cgagatcata taccgcattt gcctgcactc tcgtaccagt
    81481 atcctttccc agcgattgca tagtggcatg ctcatcgagg atagtgtcaa tggcctgcgt
    81541 gtcctccata acaataagcc cggaaccatc atccagttca taattttttg acacaccatc
    81601 ttttatacta aattggcctt tactgtttgt accactcact tccagcgcat tcgttgagac
    81661 aattaatttt ccgcctgctt ttttatcaac gccagttgca gtcccgccat ccataacctc
    81721 cagtaagccg ccactatcta cagtagtatt ataagcgaag tcattttctt caactcgcaa
    81781 acttccgccg ttttccaaca gcatattgtt agcaatacca ttcttgattt cgaactgacc
    81841 gagacggttt gttccgaata cctccatgac ccgcgtgctt gctttaatag caccgcctgc
    81901 tttctgatct accgcaatcg ctaatccccc ctctttaacc tccagtaatc ctcccttttc
    81961 gatagcggca aggacggtaa tcccatcttt ttcaacgatt aagcgcccgc ctttaatcgt
    82021 gggatcattc gctgcgccat aaacctgtag cacaccatta ttgagcgtag tctggtttgc
    82081 cgcgccaccc atttgaacaa tttgataacc atcatttaat actgagtatt ctgcggtgcc
    82141 attcatttca atgtactgat acccgccgtt aatttcagta ttactactta taccaaattc
    82201 tttaatatgc tgttctccgc caggattgat tttcgtatta ttggcggtcc cataaaccag
    82261 ctggatatct ttttccagga caacaccatc aactgtctca ccatactcaa ctgttgatgc
    82321 caatgctatg ggtgaatata acgctgtagc taccagaaca gatattgcgg aacgacaaag
    82381 agaaacagaa ccagattgat gcattgagct ttcatcctat gaaattaatt gcagttaaaa
    82441 gcattgggta cagaaaatac ccatagctcc atacccggag tcagttttta aaaactgttt
    82501 aaagaaatgc acaagtattg tgattgattt tttagttgtt tttcttgatg agaagctgat
    82561 gcaaaattcc gtctttataa tgaaaatgat gccaaagcga acgacaaggt tgtagttttc
    82621 gctacatgtc catacataaa atggggtaac attcacgcgc ctggtagcgt taccaacgct
    82681 acgctcaaac ataatgattc taataaaacc tcaggagact actatgcctg aagcaacgcc
    82741 ttttcaggtg atgattgtgg atgatcatcc acttatgcga cgcggtgttc gtcagttact
    82801 ggagcttgat cctggctttg aagtggtcgc cgaagcgggc gacggcgcga gtgctatcga
    82861 tctggcgaat agactggata tcgacgtgat cttgctggat ctcaatatga aaggtatgag
    82921 tggcctggat accctcaatg ccttgcgcag ggatggcgtt accgcgcaaa ttattatcct
    82981 gaccgtatca gatgcctcca gcgatgtctt tgcactgata gacgcaggcg cagacggtta
    83041 tctgttgaaa gacagcgacc cggaagtgtt gctggaagcg attcgtgccg gagcgaaagg
    83101 cagcaaagtc tttagcgaac gcgtcaatca gtacttacat gaacgtgaaa tgtttggcgc
    83161 ggaagaagat cccttcagcg tgctgacgga gcgcgagctg gatgttctgc acgagctggc
    83221 acaggggctg tcaaataaac agattgcctc ggtgttgaat atttccgagc agacagtaaa
    83281 agtacatatt cgcaatctac tgcgcaaact caatgtccgc tcacgcgtgg cagccaccat
    83341 tctgttcctg caacaacgcg gggcacaata aaaatagccc gatggcatca ccatcgggcc
    83401 tctttttatt tactctcctg cggcgacaaa tgttgcatcg cctgcgcgat actacgttca
    83461 atcaccgcac ggcgagtatc gttggcaggt aagagtttca acatcatctc ccacgcggca
    83521 acggcttcgc caaatcgctg ctgctcaaag gcattaaacg catacatgct taacacacga
    83581 atattgctat ggtccgttct caccaactgg cgcagcagtt cgccgccgag gcggttgtcg
    83641 ttgggatcag atgaacgtgt caacgcttca gcataaccca gtgtggcatc gctgtttttc
    83701 ggatcgaggc gatacgcagt agcgtatgca tcggtggcga tactggcgtt acccagcgcc
    83761 atgccaacgc gacccaacat aatccaacct tctatatctc ccggattttt ttgcagttga
    83821 gtacgcatcc ccagcgcaag gcgcgacatc tcttcttcgt tgagcggatc ggctttcgga
    83881 tccagcgccc tgtccagcaa cgccggagcc tgtgccgtgg cctgctgcca gattttcacc
    83941 tgctgataat tgccagtctg gtagtagctg acgccagcca caattaacgc caccacaata
    84001 cccggcagat aaacaacata tccggcacgc ttaccttccg gcacgctttg ttcaggaaac
    84061 gcttccggta ccacgcgtac ccgacgccgc gaacgggcgt atatgaccca accgccaata
    84121 ccaatagcca ctaccggaag cacccacagc agcacggtca gcggcgttaa cggcggatcg
    84181 taagtgacga agttgccgta acgcgccacc atataatcga caatctcttt cttacttttg
    84241 ccttcctgca tcagttcata cactttctgg cgcaggtcgg tggcaatcat cgagttggaa
    84301 tcggcaatgc tgttattctg gcatttcggg cagcgcagtt cttcagtgag ctgacggaac
    84361 tgttgttcct gtgcttcatc tttaaactgc aacacgtcga tggtcgccag cgctgagccg
    84421 gagatcatca gcatcagcac gcccaataaa aacctcattg tgcggcctcc ttactgtatt
    84481 tctcccacag cggcttgatc tcttcttccc agacgcgagg attgagatcg cccgcatggc
    84541 gatagcgaat gatgccgttg ccgtcaataa gaaacgtttc tggcgcgcca tagacaccga
    84601 gatccagccc taacatgcca tcgccatcaa aaaggcttag cgcgtaagga ttgcccagct
    84661 ctttcagcca gctgattgcc ttctggcgat cgtctttata gttcatgccg accacgcgga
    84721 tgccctgcgc agaaagctga ttcagatatt gatgttccgc acggcaggtc ggacaccagg
    84781 tcgcccagac gttaagcagc actggtttgc cctgagtcag cacatccgcc tgataaaact
    84841 gccccggatt gtccagtgat tcgagacgaa acttcggcac aggcttgcca atgagcgccg
    84901 attccagatt ggtcggatca tccccttcgg cattacgcgc cagctgccac agcagcgccg
    84961 cggcaatcgc caggaagata atcaacggaa ttaacaatac tttgcgcttc atacagcctc
    85021 cggcgcagtt ttttgcgggc tcacgcgctt acgatagcga ggatcaaaca gacacagcag
    85081 tccgcccaac gccatcatca gcccgcccgc ccaaatccag cgaacaaatg gtttgtagta
    85141 aagacgcaca gcccacgcgc cgttttccag ctcttcaccg agcgcggcgt acaggtcacg
    85201 cgtgatgccg ccgtcaattg ccgcttcggt catcatcgac ccggcagtgt tgtaataacg
    85261 tttttccgca tacagcaccg tttccggttt accctctcgc gttacgccga tagtcgccac
    85321 accgccacgc cagttcgggc cagtcacctc tttgacatca cggaaggtga agcgatattc
    85381 atgaatatcg acgctatcgc cggacttcat gcgcacatca cgctcaacgc tatagttctg
    85441 gctaaaggca atgccaacaa ttgtcactgc cagccccagg tgagccgcca ccatccccca
    85501 ataactgaag gtggttttcg tgccgcgtga aatacgtagc gcagcttccg caattgccag
    85561 caccgcaatc cagcaggcca ttgccaggcc gagcaccgtc atcgccacaa ctttgctttc
    85621 gaacagccac ggcaacagca gagacagcac cagcgtagag atgaaggcga taatcaataa
    85681 attgcggatt ttacgtgggc gatcgcgccc ccagcgcacc agaggaccga cacccagcag
    85741 tagcgcaaac ggcaccatca gccaggtaaa catggtgttg aagaacggtt cgccaatcga
    85801 aatactgccc agtcccagtt gcttgtgcac cagcggcagc agcgtcccca gcaacaccac
    85861 cagcatcgcg gcgaccagca aaacgttgtt cgctaacagc aaagattccc gcgaccacag
    85921 cgcattgttt acgcgtgagc gaactttgtg tccacgcgcg gcaaacagca gcagcgaacc
    85981 gccaatcacc agcaccataa aggcgaggat aaacataccg cgcgccggat cagacgcaaa
    86041 cgcgtgtacc gataccagca cgccggaacg cacgaggaaa gtccccaaca gacacaacga
    86101 gaaggcactg attgccagca gtaatgtcca cgctttgaag ctggcgcgtt gttcagtgac
    86161 cgccagtgag tgcatcagcg cagtccccac cagccacggc ataaacgagg cgttttctac
    86221 cggatcccag aaccaccagc caccccagcc gagttcgtaa taggcccatg cggaaccgag
    86281 cacgatgccg agcgtcagga agatccacgc tgccagcgtc cacggacgag taaaacgcgc
    86341 ataagtgctg tccagacgcc cgctcagcaa agaagcaatg gcaaaagcaa acgccaccga
    86401 gaaacccacg taccccatat aaagcagagg cggatggaag atcagccccg gatcctgtaa
    86461 cagcgggtta agatcgcgcc cttcaatcgg gaagttcggc agcgtgcgtg agaatgggtt
    86521 agaggtaaag agaatgaaca gcaaaaagcc gacactgacc atccccatta tcgccagtac
    86581 gcgggccaca atatccagcg gaatacgctg actaaaaatc gccaccgcaa aggtccagcc
    86641 gctcatcagc agcacccaca gcagcagtga gccttcatgt gcgccccagg tagccgccac
    86701 gcgataccac accggaagct gggtattgga gttgctggca acataggtga cggtaaagtc
    86761 gttgaccacg aaagcattga ccagcaccag aaatgcgcca gccacagaca taaacagcag
    86821 ccaggcaaac aagcgggaag acgccatcat gcgcgcatct ccgcgcgcca cgccccatag
    86881 cggatacacg gacagcagca gcgcaattcc cagcgccagg cacagcagcc cgttaccaat
    86941 ttccggcatc atgatgctgg gtccttataa acactcgccg ggcgacggtg gttagcttcc
    87001 atcgctttct caacttctgg cggcgtgtag ttttcatcat gtttcgccag cacttctttt
    87061 gcgaggatat gattgccttt ttccagctcg ccctgcacca caacgccctg cccttcacgg
    87121 aacagatccg gcaaaatgcc ttcgtaagag acatccactg agccttcagc gtcgtaaatg
    87181 gtgaaggtca ctttcagcga attgggatcg cgctgcacac tacccggcat caccatcccg
    87241 ccaacgcgca gacgctgacc gacttccggc atctgctgag tttcacgctt gccgtagaga
    87301 atttcccccg gcgtataaaa gagatcgata ttcgagcgca gcgcatatag caccagaccg
    87361 atagtcagcg ccagccctgc caacacggca caggcaatcc acaagcggtt tttacggcga
    87421 atattcatgc agcctcctgc tgttgcgcag cacgtaaacg cgcctcacgc gcccgctgtt
    87481 gcgccacacc acgcagaatt gcgcgatgct gcatcaccga gtgcacgacc aaaaccacca
    87541 gcggaataac ggtcatcacc accgccagcc agacaaaaaa ggcgtaaccg cccattgcga
    87601 aaaattcatt ccaggaagca aatgcagggg tcatttacgg cctcttttca gtatcagttc
    87661 actcacccac ggacggcgtt tttccatcag caaaatcaaa ttacgcatcc gcatcagcgt
    87721 cagcgtggca gagaggagca ggaagccaaa aatcgaccag cgcagcggcg aacgcatcgc
    87781 cggatcgata ctttgctgca tccgcgttga tccctgatgc agggtgttcc accactccac
    87841 ggagtaatga ataatcggca gattcaccac gccaatcagc accaggatac ctgccgcacg
    87901 gcccgccaga cggcggtcgt cgaaggcgtg ccacagggca atcacaccca catacaaaaa
    87961 cagcagcacc agttcagaag tcagacgtgc atcccatacc caccaggtgc cccacatcgg
    88021 ttttccccat gcagagccgg taaccagggc aataaaggta aacacggcac caatgggggc
    88081 catcgccgcc accgccaggt tggccatttt catctgccag acaaggccaa taaacgccgc
    88141 caccgccatt gatgcataaa tgcccatcga ccagatcgcc gcaggcacat gcaggtagat
    88201 aatgcggtag ctatttccct gctgataatc agccggagca aagccgaatc cccagatcca
    88261 gccgacggta agcacgacta cactggcaat tgccagccac ggtataaacc agccacagat
    88321 ttgatacagc cgtggtggga tcgccagttg atgcagtgtt ttccacatag tttcgatacc
    88381 agactcgaac aaaaatcagt aatccagcgt tattgaatgc tgattcgtaa cgctgccgcc
    88441 gtcgcaaaag gacttaatgt cgcggtgcct gccagcaacg cgcctaaaat tgccagatac
    88501 ccgtcaacgg gcaaatgcat agaagccgcg tccatcgcgg cggtggcaaa gatgagtaat
    88561 gggatagtca gcggtaacac cagtatgctg agcagcacac caccgcgctt aagtcccact
    88621 gtcagcgcca cgcccggtgc accgagaaag ccaagcgtag gcgttcccag cagtagcgtt
    88681 aacgccatca cttgccagcc ataaacatcc attcccagta gcattgctac cagtggcgaa
    88741 aggatgagta acggcagacc ggttaccatc cagtgcgcca tcaccttcgc cagcacaacg
    88801 gcgggcaagg gtaacggcaa caacatcaat tgttcaagac tgccgtcctg caaatcgtca
    88861 cggaacagtc gttccagcgc cagcaaggat gaaagcagcg cggcaaccca gataatgccc
    88921 ggtgcaatac gtgccagcag ttgcggctcc ggaccgatac tgagcggaaa aagggtaatt
    88981 acaatcagga agaaccacag cgggttggcg atttcagcgc tatggcgaaa cgctacgcgc
    89041 agctcaagac ggaaaatgcg ccagaacatc atacggccct cgtttgcgtc agtgaaatgc
    89101 ggcgaatttt actttcagca acgttgagcg gctggtgggt agtgagaatc acaatccccc
    89161 cctgctctgt atgctgcgcc atacgctggg tcagacgatc gacgccgtta acgtcaatcg
    89221 cggtaaaagg ctcgtcgagg atccataacg tggcacgggt cagccacaga cgcgctaaag
    89281 cgacgcggcg ttgttgcccg gcagaaagtt gatttaccgg aatatcttcg aatccggcaa
    89341 gcccggcctg cgccagggct tccagacatt gtgcggtatc gccatcgcga tgataaaagt
    89401 gcagattttc taacgccgtc agccgggttt tgatccccgg ctgatggcct atccataaca
    89461 ggttttgatg gtagctgtcg cgtacctgat gcaagggctg cccttgccag agaacctcgc
    89521 ctgcgtcagg gcgagacaac cccgtcagca aacggagaag cgttgtcttc cccgcgccgt
    89581 tgctaccggt gatttgtacc cactctcctg cgttcagcgt aaatgacaag ccactaaata
    89641 aggttcgttc atcccgctca caaagtaact ctctgacttc aagcataccc acgcaataac
    89701 cctgttaaaa acctggctcg acttcacgca tatcgggcag cttgtgcgct atccctttat
    89761 ggcaatcaat acaggtttgc ccatctttca ccgcctggtc atgcatcttc gcggcaaccg
    89821 atttctgggc ggttgtatcc atatactcga agttgtgaca gttacggcac tcctgcgagt
    89881 tattgtcctt catgcgccgc cactcattct gtgccatcgt cagacgatga gcttcaaatt
    89941 tctgcggcgt gtcaataacg ccaaaaattt taccatacag ctctttactt gctttgagct
    90001 tgcgtatcat cttcggcaca aactcgtgcg gaacgtgaca atccggacag gtcgcacgga
    90061 cgccgctacg gttgttgtag tgcacggaat ccatgtattc ctgatacacc gtgttacgca
    90121 tttcgtggca gctaatgcag aactcttcgg tattggcttt ttccatccca gtgttaaagc
    90181 cgccccagaa gacgatgccg ccgacaaaac cgatcaacag cagcgtcccc agcgccagac
    90241 ggctgggggt acgccaccat ttccacaggc gcttaatcag accaggctta cggtcagaat
    90301 ttcccataat aacctcttat ttcccgtaac cttttgatgg ggtaaaggta ttccccacga
    90361 ttggcgcggt atcggcctgc ggtacgtgac attgcagaca gaaataacga cgcggagcca
    90421 cttccgcgcc cactttgccg tcgctgtcca taaagtgagt aggactgata cgcggcgcgc
    90481 cagtggtgcg atagctttcg acaccgtggc actgcaagca gcggttggta ttggtcgtta
    90541 cctgataacc ttcaacgcta tgcgggatca ttggcggctg attcacatag ttcagcggca
    90601 tccgatcctg ctcttttggc atccgaatgg ccccttcctg cgtcccggat acttccggcg
    90661 actggctaaa atcgacgcca ttagccgccc aaaccgcgcc gcttaccacc agggccagca
    90721 tcgccgtcca ttgacacagc gctttcttca ggtcatggct tttcatgatt tcgctcccga
    90781 actccatcgt gtagttattg taaatacatc ctcagaacag acatccacgc agcgaccgca
    90841 cgtcatgcaa tcgcggctgg tgacctgcac cgggctttgt tcatccagca ccggggcacg
    90901 tagcacatgc ggttccgggc aaacatgaaa acaatccata cagcggttac atttctgccg
    90961 atcgctggcc gcaacggtaa tcacaccttt gctacccagc acgccataca gcgcgccaac
    91021 cggacaaatg tgcccgcacc agccgtgttc aacgaccagt agatcaaata aaaacagcgc
    91081 gagaataagc agcgcgccgc tgccgaagcc cattaccagg ctacgcccca tcagggaaac
    91141 ggggttgatc cattcccaga tgagcgtgcc agttaaggct gatcccacca gaatgaccac
    91201 caacagcacg tagcgaatgt ggcgagggat cgtcgcagac tgattgaggt caaagcgcct
    91261 gcgtaaccag ttcgctaagt cggtaatcgg gttcagcggg cagacccagc tgcaaaataa
    91321 tcgctttcct gccagggcgt agagcacggt gataatcacc gcaccggtca acgccaccgt
    91381 ggcaggcaga tgaccactgg caagactttg cagcgtcatc agcggatcgg tcagcggaac
    91441 ggtgtcgaat aacaggctac tgctatagtt gccgtgcaag atccacacac caaaccacgg
    91501 accgctcaaa aacatcccca gcacgaagaa ctgacaaagg cgacgtaaca ccagccaacg
    91561 gtgactgcgc caccagcctt ttttctccag cgcctcgcgc ccggcgtcac gtttacgatt
    91621 tgccattgtt cccctccagc cagccgaagc ggtaatggtg acctaactcc cctttcgcca
    91681 gtgacagcgg taacaccttg attgccggtt gttccagcac gcatactttt tcgcacttac
    91741 cgcaaccggt acaggcgtcg ctgtgaaccg tcggcagaaa gcgggcgtgc ttaccggtac
    91801 gcgtgttgcg ctccagctcc agggtgatgg cctcatcaat tttcgggcat tcgcgataac
    91861 aaacatcgca gcgcagcccc tgaaagttga gacagttttc ctggtccacc agtaccacca
    91921 gccccatccg cgcgtcgtcg atcgattcaa tctcacgatc cagcgcaccg ctcgggcaca
    91981 ctttggcgca cggaatgtcc tcacacattt cgcaaggaat atcccgtgcg acaaaatatg
    92041 gcgtgcccgc cgacaggcca gaggccagcg tcgccagttt taaggtgtcg taagggcaag
    92101 cctgaacaca ctgaccgcaa cgcacacagg cactggcaaa ggcgttctcg tttatggctc
    92161 cgggcgggcg caaccgcacg ccagatgcgc gtgcggtttg ctgttgtaac cccagcgcca
    92221 cacccacggc agccagcccg cctgctgtgc gaacgacatc gcgcagaaag cggcggcgac
    92281 cattttgagg tttcgctgac cgggacataa tggcttacac cttctccagt ttgaccgcgc
    92341 acttcttgaa gtccgtctct ttcgagagcg gatcggtcgc gtccagcgtt agtttgttaa
    92401 ccagctgtgc ggcgtcgaag aacggcatgt acaccaggcc ctgcggcgga cggttacgac
    92461 cgcgcgtttc gacaatcgag atcacttcgc cacggcgaga aaccactttc actttgtcgc
    92521 cacggcgcag atcgcgcgct ttcgcatcca gcgggtgaat aaacaggacc gcttccggga
    92581 aggcacggtg cagttccggc acacggcgag tcatactgcc ggtgtgccag tgctccagga
    92641 cgcgtccggt agagagccac aggtcgtact cttcatccgg tgcttccgcc gccggttcga
    92701 acggcagcgc gaagatcacc gctttaccat ccggtttacc gtagaactta tagccttcgc
    92761 ccgctttcac gtacgggtcg ttaccttcgc tgtaacgcca ctgcgtttct ttaccgttca
    92821 ccaccggcca gcgcagaccg cgcgctttgt ggtagtcatc gaacggcgcg agatcgtgac
    92881 cgtgaccgcg accaaaccat gcgtactctt cgaacagccc tttttgcaga tagaagccca
    92941 gctcgcggga ttcatcgttc agctgatctt ccgccagttc ggataccggg aatttgctca
    93001 cttcgggggt ggcatacaga acttcgtaca gcgttttgcc acgcagttcc ggtttcttcg
    93061 ccagcagctc ttccggccat acttcttcag ttttgaagcg gcgggagaac tggactaact
    93121 gccagaggtc cgatttcgct tcgcctggcg cctgtacctg ctgacgccag aactgagtac
    93181 ggcgttcggc gttaccgtaa gccccctctt tttctaccca cattgcggtc ggcaggatca
    93241 agtcggcggc cagcgcactg actgtcggat acggatcgga gacgatgatg aagttgcgcg
    93301 gatcgcgcca gcccggcata cgctcttcat taatgttcgg cccggcctgc atgttgttgg
    93361 tacacatggt ccagtaaaca ttcagcttgc cgtctttcag cgcacggtct tgtgctaccg
    93421 catgcagacc gattttcgcc ggaatggtgc cgctcgggat attccacttc ttctcgcaga
    93481 tatcacgatg tttctcgtta gttaccacca tgtccgcagg cagacggtga gcaaaggtgc
    93541 ccacttcacg cgcagtacca cacgcggaag gctgcccggt cagggagaac ggaccgcaac
    93601 ccggctggga aattttgccg gtcagcaggt gcaggttgta gaccaggttg ttggcccaca
    93661 cgccacgagt atgctggttg aagcccatcg tccagtagga gatgactttc ttgttcggat
    93721 cggcatacag ctgcgccagt tgttccagct gatcttttgg tacgccagtc atttcagcgg
    93781 ttttttccag cgtatactca gcaacgaagg ctttgtaatc ttcaaagctc atcggttcgg
    93841 aggcgtcaga acccggattc ttcgctgctt tttccagcgg atgggtcgga cgtaaaccgt
    93901 agccgatgtc cgtcgcccct ttgcgcaggt taacgtgctt gctgaagaag tcctgattta
    93961 tcgcattgtt ttgaatgata tagttggcga tgtagttcag gatcaccagg tcagattgcg
    94021 gcgtaaagat gatgccgtta tccgccagct cgaagctacg atgctggtag gttgaaagca
    94081 ccgccacggt gacgtcctgg ttagagagac gacggttagt gatgcgtgac cagaggatcg
    94141 ggtgcatctc cgccatgttt gcgccccaca gcacaaacgc gtcagcctgc tcgatgtcgt
    94201 catagcagcc catcggctca tccataccaa aggttcgcat aaagccaact actgccgacg
    94261 ccatacagtg acgcgcattc gggtcgatgt tgttcgaacg gaagcccgct ttaaacagct
    94321 tggacgcggc ataaccttcc cagatagtcc actgaccaga accgaacata ccgatcgatt
    94381 ccggcccttt ttctttcagg gcggttttga atttctcttc catcacatcg aaggcctgat
    94441 cccaggtgat tggggtaaat tcgccttctt tgtcatattt accgtttttc atacgcagca
    94501 gcggctgcgt caaacggtct ttaccgtaca tgatcttggg caggaaatag cccttaatgc
    94561 agttcaggcc acggttaacc ggtgcgtccg ggtcgccctg acaggccacc acacgcccct
    94621 gctgcgttcc gaccagaacg ccgcaaccag taccgcagaa acggcacggc gctttatccc
    94681 atttaatggc ttcctgctga ccaacaacgg cgcgggcaac gcccggcacg ctgagaccgg
    94741 cagccgccgc agcggccgca acggcgttag ctttcataaa gctacgacga ctgagtttca
    94801 tggtgtttcc tcaccttgct cttcctgctg gtgataaacc agcgacaccg ccagcacgcc
    94861 ctctacgttg cgtactgact caatggtttg gatcagcgtt tcgctgtctt ctgcttccac
    94921 caccacaatc aactgaccgc tcggcgcgtc gctgacagca acttcacagc cgggaaaggc
    94981 gttcagttgg gtgctgatgt ctgaaattcg ttcgcttttg gcctgcacaa ccaggctgca
    95041 aacttgccag ttagtgtgca tggagatact ccgcagttat ggctgatacc ggacagctgg
    95101 ctgcacatgc tccacatccg ttgcaaagtt ggctattaag ttgcggctgg tagatcccgg
    95161 agagcgtcgg gcgaaagata attgccattg gctcacagct atcctgacag cggcggcatt
    95221 caactgactg atacgccagg cacgcgtccc caatggtgaa ctgcaaatcc caggccctgg
    95281 tgtggcgcgg agaaaatagc gattcggggc aagcctgcgc gcaggcgtaa cagaagctgc
    95341 actcgttatt tttgaaatta acgctcggat agccgcccgc gccgcgttgc agaatgttgt
    95401 tttcacaagc attaatacag gcgtcgcagc gggtgcaatg ggtcagaaaa tgagattcat
    95461 caccggacca gggcggacgg ataccgttac tggctttgcg ccagcgacca gtgagtatgc
    95521 cccgacggga tgcatcaatc ttcacattga ccttccatca ttaacgcgct ctgaaaattg
    95581 agagcgacca aataaaccgc ataattaata agccattttt atagccgcta agatattaaa
    95641 ggatgtgtca aagatgcata ccccgatcga ggtaaaacct gtagcaggat caaaagagtg
    95701 gcgggaagcg tggcaaaaac gggcttttgc tcacatttca aatggttata aatatattta
    95761 tatagcaatt aattcaccag agatatttct gctggtttgc tctctcatca gaatttaaca
    95821 ctaaaagagc aggtaaaatt gtctgaatat tctttaagtt attcataaag caaattaata
    95881 aatctgatga atatgttaac cttcagcgac atcatcggtg caaacctata aatgaagaag
    95941 gaaagcaaaa aatgaagacc attctacctg cagtattgtt tgccgctttc gccaccactt
    96001 ccgcctgggc ggcagagagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    96061 aaaaaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    96121 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    96181 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    96241 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    96301 tcaccgcgta tctgggcgat actggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    96361 tgtatacgcc agacaatgta gatgtgaagt accgcgtctg gaaggcggaa gagaaaattg
    96421 acaacgcggt agttcgctaa actgccgtga agtgcggcac cccgtaggtt ggataaagcg
    96481 gtcacgccgc atccgacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    96541 ttatcagcat acgccacatc cggcatacca tgccggatgt ggcacatcat tacaacgcaa
    96601 tatccgccac ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg
    96661 ctgcttgcgg tttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt
    96721 ctgttgccgc tacatcaccg ttcagcttgc gtccataaga tggaacgatc gctttcaacg
    96781 tagcctgcca ttgcgggctg gaaacgcgat cgccaaatac tttttccagc agattcaaca
    96841 taatcggcgc ggcggttgac gcccctggcg atgcccccag gagcgcggca atggttcctt
    96901 gctggtcact gaccacttca gtacccagac gcagtacacc acctttatcg gcatcacgct
    96961 tgataatctg cacacgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcttgcg
    97021 gatagtactc tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca
    97081 gatatttcac cagatcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg
    97141 tggtggtgga actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg
    97201 tggcaaatgg cccaaacagc actacgcgtt taccgtccag aacgcgggta tcgatatgcg
    97261 gaaccgacat cggtggtgcg ccaacggatg ctttaccgta aaccttcgcc agatggtgat
    97321 taaccacgtc cgggttttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt
    97381 ctttcgcttc cggaatcccc gattcctgta acagcttcag tgccgcaccg cccgcgccga
    97441 taaagacaaa tttcgcacgg atgttctgtg cagtgccatt tttcagatcg gcaacggtaa
    97501 cggtccaggt gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg
    97561 agaagttaga tttcttctgc aaggaagcaa ttaactggcg ggtgatctcg ccgtagttca
    97621 catcggtacc aatttccgta cgcgtggctg ccactttctg ttgcggatcg cgcccttcca
    97681 tcactaacgg tgcccactct ttgatctgcg cgtgatcttc agagtaacgc ataccgcgaa
    97741 acagcgagct ttgttgcaac gcggcgtaac gggcgcgcag gaaattgacg ttatcctcgc
    97801 cccagacaaa gctcatatgc ggaacggtat tgataaatga acgcggagta cgcagcacgc
    97861 cgcgctcgac ctggtgcgcc cagaactggc gggaaatctg aaatgcttcg ttaatggcga
    97921 ctgctttttc aatactgatg ctgccatcgg cgttttgcgg ggtgtagttc agttccatca
    97981 gtgcagaatg cccggttccg gcgttattcc agccgttcga actctcctgc gcgacaccct
    98041 ccaggcgctc caccatggtc atcgaccatt caggctccag ctcgcgtaaa taggtcccca
    98101 acgtggcgct cataatgccg ccgccaatca acagtacatc agtttcctgc tcctcggacg
    98161 ctttcgcttt tgccgccatc gaaacggcat taagccccac ggccatcgag aagagcatgg
    98221 cagtcacttt tttcatcttg ttaatgcctt acttttagtc gctttattgc aggtgagatt
    98281 tgcgcggcat caacggtaac acttaagtaa caacatttaa ataatgttta taaattatat
    98341 tctaaattta gaaacattat aatttcgttg attaattata gggttattag aaatgaaggg
    98401 atttttagca tattgacctg gcggcagcag tccgccaggt cagcgtcgta atattatgcc
    98461 gtccgggcaa cggcatcacg cgaagcggca tcgcgctctt cgcccgtcag ctcgctaagt
    98521 tgcccgttgc gcatttccag caggcggtcg gcgtggataa agtaatgatc atcatgactg
    98581 atggcgaaaa tagttttacc catctcctgc atcagcggca gcaacacctg ataaaactca
    98641 cgacggaagt gtggatcctg atccgccgcc cattcatcca gcaggataat atcgcgttct
    98701 tctgccagcg ccagcaacag cgccacgcgt tttttctgcc cttttgataa cttcaggtta
    98761 acaatacgcc cgtttcttaa ctcaagctta tgagccattt tcagctgcgc cagccacttc
    98821 tcaaccagtt gcgggttagc gggtttaccc tccggcccca gcagttgatc aaacagccag
    98881 acatcggtaa acactgccga aaacagtttg cgataatctt ccggttgttc gccgctgaca
    98941 ggtttgccat ccagcaagat ttcgccgctt tgtggctgat acaagcccgt caacaacatc
    99001 gccagcgtcg attttccgct accgttgccg ccaatcagaa acagcagctc gccacgtttg
    99061 atggtgagat taatcggacc aacggaaaac gcgttatcct gataagcaaa cgtcacgtta
    99121 cgcagctcca gcgtttgcca gctgggaaac gcctgcgggc gcggaaactc tgctttgaaa
    99181 ggcgcgagcg cgaatttgtt cagcttgtta aacgccacct gcgccgtcag cagcgtcggc
    99241 aatgcgccaa ccgccgaaag cagcggcgta cgcaggaata aaagcgtcaa cgaataggtc
    99301 gcggcaacgt tggtatcagc ccaaccgaga ctgttcgcca tccagaacac caggccgata
    99361 gcgcccagca tcatgatgtt tgaccagttc acggcactaa gatggaaggt gtctgcgcga
    99421 ataatatgat ggcgatactc ttgcgcatca ggaatgtaga ggttgttaaa cacatactcg
    99481 gcgcgttccc ggttcagagt cagctctttg cgcccttcaa gtacagtttg aaaatccgtg
    99541 tacagcttgt cttcggtttc acgcagggtc gccatatgtt tgtacacccg cgccaccagc
    99601 acaaaaccgc cccagatggt gactgccatc cagatagccg ttaccagcaa cattttgccc
    99661 gacagcatcc acagatacgc cgctgaaccg atagtgagaa tgatcccctg caccagttcc
    99721 ggcagacgca caaaagcaat ggtgatattg cgcacatcgc tggttaatcc cgccagcaac
    99781 gaggcgctac cgagttgttc aatgcgctcg acgtgagtat ccagaatccg cttgataaat
    99841 tcactacgca gtcggtaaac gaagtgatgc cccaaagtgg tgagcgccag ttgcgatccg
    99901 agagtgactg ccatcaacag cagcaataat cccagaaact ccggcaacac cagcagactg
    99961 gtatccgccg tttcgataag gcgctgattg ataaaagcaa ttaagccaat gcctaaagcc
   100021 gcactggcga ggcttagcgc catcacactg ataaatggcc agcgatactg ccgccagaca
   100081 agtacaagaa gttccatgca gaaaacccgg acaatgaatt acagcccgca gtttaaacat
   100141 cttcgcgcgc acagcaataa taattcttat ttttattctt ttttacctgc ctgacggaat
   100201 gtcaggttgt agcggcagtc ggtggtgagt ggatgaaacc ccgctttcaa cggttgaata
   100261 ccgtgataaa acagccgcga ttcaccgccc cataccacca catcgccatg ttccaacaac
   100321 aaacgtttga gcggatcatt acgtttcagg ccaccaaatt gaaaaatcgc gggtaagccc
   100381 agagaaacag aaacaattgg cgcgcgcaga tccggttcgt ctttatcctg atgcagcgac
   100441 agtttcgcgc caggagcgta gcggttgata agacaagcat ctggctggaa atctggatag
   100501 cccgccgccg tagccgcacg ttgacataaa ttatgaaaac tctgcggcat ggcgggccac
   100561 ggtttatttg tttgcggatc aatgggcgaa tagagataac cttgccgatg ggtcgtccag
   100621 cccagatgcc cacagttggt catcgccacc gacatggtat atcccccggg ggtgaccatc
   100681 tggcgaaacg gcgactggct ggcaacgtca ttaatatcgc ggattagttg ctctgcagcg
   100741 ttaaaagcaa aacgccgtaa aattaccgca ccagccgcca gtggctcttg ccacggttca
   100801 gcatcggcaa acagatccaa cattacctct cctcattttc agcttcgcgg cgcagcagtt
   100861 gcgctttacg cgacacgccc cagcggtaac cggaaagtgt gccatcacca cggaccactc
   100921 gatgacaggg aattacgata gccagcttgt tggcggcaca ggcgctggca acggcccgta
   100981 ccgctttcgg tttgccgatg gcgttagcca gttgctgata actgacggtt tcaccgcaag
   101041 gtatcgtgcg cagtgcctgc cagacttgtt gctgaaaagc agtgccgcga atatccagcg
   101101 gtaacgtcag cggagtatcg cgttgattga ggctggcgat cacttcacgc acatgttgct
   101161 gaaacgtcag atcggcaggc gcgttgtcgg cagcgggaaa catctgctgc aactcgctga
   101221 ttaatgtcgc gtcatcatcg cccagcaata tcgcgcaaat cccccgctcg ctttctgcca
   101281 ccaggcaacg acccagctca caatcagcca gcgcgtaacg caccgccaga ttttcgccac
   101341 cgtgacggaa ttgtttagcc gtcatgccca gcgtttcatc ggctttgtgg tagtaactgc
   101401 tgctgtcggg gaatccggcg ttaagaatag acgtcgtcac gctctcccct ttcgccagcg
   101461 attcgcgcaa acggcgagcg cgccaggcct gttgccaggc tttaggcgtc attccggtag
   101521 tcgctttaaa caaccgatgt agatgaaatg gactcatcgc cacctggtcg gctaaggctt
   101581 ccaacgttac aggcgtttcc tgttccagca gtcgacacgc gtgggtgatt ttatccaacc
   101641 gatgttgctg ggcattggct ttatcaggtt ggcagcgttt gcaggggcga aatccggcgg
   101701 cgagtgcctc gctggcattt gcgtagaagg agacgttttc ccgcaaagca tgtctggcgc
   101761 ggcaagacgg acggcaaaag atgcctgtgg tgcgcacggc gaaaacgaat tcgccgtcgg
   101821 cattcgggtc gcgggctaag acagattgcc agcgttgatc gtcagttaag catgtggcgt
   101881 ttttcataat cagctccctg gttaaggata gcctttagtc tgcccggcca ccatcacgca
   101941 aaaaccaaca atcttgcgct ttaatttttt tcgctgacaa ggaagctttt aaactgtggt
   102001 gacatccagg ttttaaagct atcgccttct ttggtgatca tataaaccgc cagcccttcc
   102061 cggcgaacaa cttctttggc tttctccggc ccgagtacca tcaaacctgt atcccaggcg
   102121 tcggcttcca gcgccgtcgg tgcaatcacc gtcacggata ccagattgtg ttcgatggga
   102181 cgcccggttt gcggatcgat aacatgggaa agacgtttgc cgtccagttc gtaatagtta
   102241 cggtagctgc cagaggtgct aatcccatga ccgttgatat ccaccacggc ctgaaccgcg
   102301 ttttctttat cggttggttt ttgaatcgct acccgccacg gcaggccttc accgttcata
   102361 ccacggctgt tcagcgcgcc gcccaccgac accagatagc gggaaatccc ttcctgctcc
   102421 atcaagcgtg ccaggtgatc cgccgcataa ccttcgccga cggtggagag atcgacatat
   102481 aaatccggca ggtctttttg cagatattgc tgatgcgact gattaatgac cgtaaggtgc
   102541 tgtaaaccag ttttggcttt catcgcgtcg atctgttcct ggctcggaat ttgaaccggc
   102601 tgttgttccg gaccgaaacc ccacagattc accagcggcc cgacggttat atccatcgcg
   102661 ccatcggtct tcgcgccaat gcgtagcgag gtggtgacga tatcggccat cgcttcactt
   102721 accggccacg gcgataaact ttgcgagtcg ttaaagcgca tcagcgcgga atctttttta
   102781 taggtcgaaa gcagctgatc gtcggcgtcc agctgggtct gaatcttttc tttaagttcg
   102841 gcgctgcgtt tggcgtcaat gcccgggatg ctggcacgcc agaaggtacc catagttttg
   102901 ccttcaagaa cggtaacttc agtagcgtgg gttttggcgg gttgtggttt ttgatcgcaa
   102961 ccaacaaaga agagcgcggc agccagcagt gccacgcggg taaagcttat ttccatacgt
   103021 gattatcctc atgcgaacgg tcgcaagagt acaccaaaaa actgagtttg tacgctgaaa
   103081 acaatgaaaa aagggcccgc aggccctttg ttcgatatca atcgagatta gaactggtaa
   103141 accagaccca gagctacgat gttatcagtg ttgatgccag cgtcacgagt gaactggttg
   103201 tcgtccagca ggttgatttt gtagtcaaca taggtggaca tgtttttgtt gaagtagtag
   103261 gtcgcgccaa catcaacata tttcaggata tcttcgtcgt cgtagttacg accattgatg
   103321 acacccaggt ttttaccttt agactgcagg taagccaggg acggacgcag accgaagtcg
   103381 aactggtact gagcaacagc ttcgaagttc tgtgctttgt tcgcccaacc cagggaacct
   103441 acgcgagttg cgttgtaggt ctgggtgtac tgagcagcca gatagatgtt gttagcgtcg
   103501 tatttcagac caccagtgta ggtttcagca cggtcgccgt taccgatgta cagcgggctg
   103561 ttctgatcat cagtacgttt ggagctggaa actgcagcac cgataccgaa gccttcgtaa
   103621 tcataagtga tagatccgcc aacgccgtcg ccgttctgac gcagtgcttc acgaccgttg
   103681 ttagtcatgc cttcgccgct tacgctaccg tttttgccct ggtactgaac agcaaagttc
   103741 aggccgtcaa ccagaccgaa gaagtcagtg ttacggtagg tcgcgaaacc gttaccacgc
   103801 tgctgcatga agttgtcaga accgtaggtg tcaccaccga attctggcag aacgtcggtc
   103861 caggaagtta cgtcgtaaac tacgccgtag ttacgaccgt agtcgaaaga acctacatcc
   103921 tggaatttca gacctgcgaa tgccacacgg gtccaggagt tgttttcgtt ttcagcgctg
   103981 ttgccctgga tctgatattc ccactggccg taaccggtca gctggtcagt aacctgagtt
   104041 tcacctttga agccaagacg catgtaggtc tggtcgccat ctacagactt gtcgtcagag
   104101 aaatagtgca ggccgtctac tttaccgtac agatctaatt tgttgccgtc tttgttgtaa
   104161 acttcagcag cgtttgctgc gcctgctacc agcagagctg ggaccaggag ggacagtact
   104221 ttaactttca tgttattaac cctctgttat atgcctttat ttgctttttt atgccactgc
   104281 atactgatta accctcatta atcagtcggc aagtccattc tccccaaaaa tgcagaataa
   104341 tccaacacga atatgatact aaaactttta agatgtttca tttatcgcta tagatgtttc
   104401 aaaatgtaaa ttcgagggaa ctttttaaga ttattgcgga atggcgaaat aagcacctaa
   104461 catcaagcaa taataattca aggt
//
LOCUS       DABSYT020000005       101857 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.005, whole genome shotgun sequence.
ACCESSION   DABSYT020000005 DABSYT020000000
VERSION     DABSYT020000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 101857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 101857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.005"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            115..741
                     /gene="ydhY"
                     /locus_tag="ID725_RS02430"
                     /old_locus_tag="pgaptmp_000486"
     CDS             115..741
                     /gene="ydhY"
                     /locus_tag="ID725_RS02430"
                     /old_locus_tag="pgaptmp_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGV7849167.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            762..2864
                     /gene="ydhV"
                     /locus_tag="ID725_RS02435"
                     /old_locus_tag="pgaptmp_000487"
     CDS             762..2864
                     /gene="ydhV"
                     /locus_tag="ID725_RS02435"
                     /old_locus_tag="pgaptmp_000487"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGV7849168.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2877..3515
                     /gene="ydhW"
                     /locus_tag="ID725_RS02440"
                     /old_locus_tag="pgaptmp_000488"
     CDS             2877..3515
                     /gene="ydhW"
                     /locus_tag="ID725_RS02440"
                     /old_locus_tag="pgaptmp_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGV7849169.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3579..4247
                     /gene="ydhX"
                     /locus_tag="ID725_RS02445"
                     /old_locus_tag="pgaptmp_000489"
     CDS             3579..4247
                     /gene="ydhX"
                     /locus_tag="ID725_RS02445"
                     /old_locus_tag="pgaptmp_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGV7849170.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICACACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4244..5029
                     /gene="phsC"
                     /locus_tag="ID725_RS02450"
                     /old_locus_tag="pgaptmp_000490"
     CDS             4244..5029
                     /gene="phsC"
                     /locus_tag="ID725_RS02450"
                     /old_locus_tag="pgaptmp_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGV7849171.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5033..5845
                     /gene="ydhT"
                     /locus_tag="ID725_RS02455"
                     /old_locus_tag="pgaptmp_000491"
     CDS             5033..5845
                     /gene="ydhT"
                     /locus_tag="ID725_RS02455"
                     /old_locus_tag="pgaptmp_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGV7849172.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5857..7461)
                     /gene="ydhS"
                     /locus_tag="ID725_RS02460"
                     /old_locus_tag="pgaptmp_000492"
     CDS             complement(5857..7461)
                     /gene="ydhS"
                     /locus_tag="ID725_RS02460"
                     /old_locus_tag="pgaptmp_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGV7849173.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTLLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7587..7892)
                     /gene="ydhR"
                     /locus_tag="ID725_RS02465"
                     /old_locus_tag="pgaptmp_000493"
     CDS             complement(7587..7892)
                     /gene="ydhR"
                     /locus_tag="ID725_RS02465"
                     /old_locus_tag="pgaptmp_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGV7849174.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8000..8076)
                     /locus_tag="ID725_RS02470"
                     /old_locus_tag="pgaptmp_000494"
     tRNA            complement(8000..8076)
                     /locus_tag="ID725_RS02470"
                     /old_locus_tag="pgaptmp_000494"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8040..8042),aa:Val,seq:gac)
     gene            complement(8081..8157)
                     /locus_tag="ID725_RS02475"
                     /old_locus_tag="pgaptmp_000495"
     tRNA            complement(8081..8157)
                     /locus_tag="ID725_RS02475"
                     /old_locus_tag="pgaptmp_000495"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8121..8123),aa:Val,seq:gac)
     gene            8465..9721
                     /gene="ydhQ"
                     /locus_tag="ID725_RS02480"
                     /old_locus_tag="pgaptmp_000496"
     CDS             8465..9721
                     /gene="ydhQ"
                     /locus_tag="ID725_RS02480"
                     /old_locus_tag="pgaptmp_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGV7849175.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTLTVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9762..11135)
                     /gene="mdtK"
                     /locus_tag="ID725_RS02485"
                     /old_locus_tag="pgaptmp_000497"
     CDS             complement(9762..11135)
                     /gene="mdtK"
                     /locus_tag="ID725_RS02485"
                     /old_locus_tag="pgaptmp_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGV7849176.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            11350..11991
                     /gene="ribE"
                     /locus_tag="ID725_RS02490"
                     /old_locus_tag="pgaptmp_000498"
     CDS             11350..11991
                     /gene="ribE"
                     /locus_tag="ID725_RS02490"
                     /old_locus_tag="pgaptmp_000498"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGV7849177.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQSGTEA"
     gene            complement(12031..13179)
                     /gene="cfa"
                     /locus_tag="ID725_RS02495"
                     /old_locus_tag="pgaptmp_000499"
     CDS             complement(12031..13179)
                     /gene="cfa"
                     /locus_tag="ID725_RS02495"
                     /old_locus_tag="pgaptmp_000499"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGV7849178.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13470..14681)
                     /gene="punC"
                     /locus_tag="ID725_RS02500"
                     /old_locus_tag="pgaptmp_000500"
     CDS             complement(13470..14681)
                     /gene="punC"
                     /locus_tag="ID725_RS02500"
                     /old_locus_tag="pgaptmp_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGV7849179.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14794..15726
                     /gene="punR"
                     /locus_tag="ID725_RS02505"
                     /old_locus_tag="pgaptmp_000501"
     CDS             14794..15726
                     /gene="punR"
                     /locus_tag="ID725_RS02505"
                     /old_locus_tag="pgaptmp_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGV7849180.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15723..16748)
                     /gene="purR"
                     /locus_tag="ID725_RS02510"
                     /old_locus_tag="pgaptmp_000502"
     CDS             complement(15723..16748)
                     /gene="purR"
                     /locus_tag="ID725_RS02510"
                     /old_locus_tag="pgaptmp_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGV7849181.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17047..17136
                     /gene="cydH"
                     /locus_tag="ID725_RS02515"
                     /old_locus_tag="pgaptmp_000503"
                     /gene_synonym="ynhF"
     CDS             17047..17136
                     /gene="cydH"
                     /locus_tag="ID725_RS02515"
                     /old_locus_tag="pgaptmp_000503"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGV7849182.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17302..18471
                     /gene="ydhP"
                     /locus_tag="ID725_RS02520"
                     /old_locus_tag="pgaptmp_000504"
     CDS             17302..18471
                     /gene="ydhP"
                     /locus_tag="ID725_RS02520"
                     /old_locus_tag="pgaptmp_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7849183.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18617..19198)
                     /gene="sodB"
                     /locus_tag="ID725_RS02525"
                     /old_locus_tag="pgaptmp_000505"
     CDS             complement(18617..19198)
                     /gene="sodB"
                     /locus_tag="ID725_RS02525"
                     /old_locus_tag="pgaptmp_000505"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGV7849184.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19326..20141)
                     /gene="mepH"
                     /locus_tag="ID725_RS02530"
                     /old_locus_tag="pgaptmp_000506"
     CDS             complement(19326..20141)
                     /gene="mepH"
                     /locus_tag="ID725_RS02530"
                     /old_locus_tag="pgaptmp_000506"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGV7849185.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20476..20823
                     /gene="grxD"
                     /locus_tag="ID725_RS02535"
                     /old_locus_tag="pgaptmp_000507"
     CDS             20476..20823
                     /gene="grxD"
                     /locus_tag="ID725_RS02535"
                     /old_locus_tag="pgaptmp_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGV7849186.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20874..25490)
                     /gene="lhr"
                     /locus_tag="ID725_RS02540"
                     /old_locus_tag="pgaptmp_000508"
     CDS             complement(20874..25490)
                     /gene="lhr"
                     /locus_tag="ID725_RS02540"
                     /old_locus_tag="pgaptmp_000508"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGV7849187.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25583..26230)
                     /gene="rnt"
                     /locus_tag="ID725_RS02545"
                     /old_locus_tag="pgaptmp_000509"
     CDS             complement(25583..26230)
                     /gene="rnt"
                     /locus_tag="ID725_RS02545"
                     /old_locus_tag="pgaptmp_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGV7849188.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26333..26740)
                     /gene="gloA"
                     /locus_tag="ID725_RS02550"
                     /old_locus_tag="pgaptmp_000510"
     CDS             complement(26333..26740)
                     /gene="gloA"
                     /locus_tag="ID725_RS02550"
                     /old_locus_tag="pgaptmp_000510"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGV7849189.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26821..27918)
                     /gene="nemA"
                     /locus_tag="ID725_RS02555"
                     /old_locus_tag="pgaptmp_000511"
     CDS             complement(26821..27918)
                     /gene="nemA"
                     /locus_tag="ID725_RS02555"
                     /old_locus_tag="pgaptmp_000511"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGV7849190.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(27955..28554)
                     /gene="nemR"
                     /locus_tag="ID725_RS02560"
                     /old_locus_tag="pgaptmp_000512"
     CDS             complement(27955..28554)
                     /gene="nemR"
                     /locus_tag="ID725_RS02560"
                     /old_locus_tag="pgaptmp_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGV7849191.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNYEPLENALAHVKTIIATPAV"
     gene            28657..28896
                     /gene="ydhL"
                     /locus_tag="ID725_RS02565"
                     /old_locus_tag="pgaptmp_000513"
     CDS             28657..28896
                     /gene="ydhL"
                     /locus_tag="ID725_RS02565"
                     /old_locus_tag="pgaptmp_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGV7849192.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            28945..29841
                     /gene="ydhF"
                     /locus_tag="ID725_RS02570"
                     /old_locus_tag="pgaptmp_000514"
                     /pseudo
     CDS             28945..29841
                     /gene="ydhF"
                     /locus_tag="ID725_RS02570"
                     /old_locus_tag="pgaptmp_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            29922..30443
                     /gene="sodC"
                     /locus_tag="ID725_RS02575"
                     /old_locus_tag="pgaptmp_000515"
     CDS             29922..30443
                     /gene="sodC"
                     /locus_tag="ID725_RS02575"
                     /old_locus_tag="pgaptmp_000515"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGV7849193.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30444..32456)
                     /gene="ydhK"
                     /locus_tag="ID725_RS02580"
                     /old_locus_tag="pgaptmp_000516"
     CDS             complement(30444..32456)
                     /gene="ydhK"
                     /locus_tag="ID725_RS02580"
                     /old_locus_tag="pgaptmp_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGV7849194.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32456..33313)
                     /gene="ydhJ"
                     /locus_tag="ID725_RS02585"
                     /old_locus_tag="pgaptmp_000517"
     CDS             complement(32456..33313)
                     /gene="ydhJ"
                     /locus_tag="ID725_RS02585"
                     /old_locus_tag="pgaptmp_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGV7849195.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33316..33552)
                     /gene="ydhI"
                     /locus_tag="ID725_RS02590"
                     /old_locus_tag="pgaptmp_000518"
     CDS             complement(33316..33552)
                     /gene="ydhI"
                     /locus_tag="ID725_RS02590"
                     /old_locus_tag="pgaptmp_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGV7849196.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33753..34187
                     /gene="slyA"
                     /locus_tag="ID725_RS02595"
                     /old_locus_tag="pgaptmp_000519"
     CDS             33753..34187
                     /gene="slyA"
                     /locus_tag="ID725_RS02595"
                     /old_locus_tag="pgaptmp_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGV7849197.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34234..34701)
                     /gene="slyB"
                     /locus_tag="ID725_RS02600"
                     /old_locus_tag="pgaptmp_000520"
     CDS             complement(34234..34701)
                     /gene="slyB"
                     /locus_tag="ID725_RS02600"
                     /old_locus_tag="pgaptmp_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGV7849198.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            34974..36083
                     /gene="anmK"
                     /locus_tag="ID725_RS02605"
                     /old_locus_tag="pgaptmp_000521"
     CDS             34974..36083
                     /gene="anmK"
                     /locus_tag="ID725_RS02605"
                     /old_locus_tag="pgaptmp_000521"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGV7849199.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            36181..36510
                     /gene="mliC"
                     /locus_tag="ID725_RS02610"
                     /old_locus_tag="pgaptmp_000522"
     CDS             36181..36510
                     /gene="mliC"
                     /locus_tag="ID725_RS02610"
                     /old_locus_tag="pgaptmp_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGV7849200.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36569..37225
                     /gene="pdxH"
                     /locus_tag="ID725_RS02615"
                     /old_locus_tag="pgaptmp_000523"
     CDS             36569..37225
                     /gene="pdxH"
                     /locus_tag="ID725_RS02615"
                     /old_locus_tag="pgaptmp_000523"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGV7849201.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37354..38628
                     /gene="tyrS"
                     /locus_tag="ID725_RS02620"
                     /old_locus_tag="pgaptmp_000524"
     CDS             37354..38628
                     /gene="tyrS"
                     /locus_tag="ID725_RS02620"
                     /old_locus_tag="pgaptmp_000524"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGV7849202.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38690..39550
                     /gene="pdxY"
                     /locus_tag="ID725_RS02625"
                     /old_locus_tag="pgaptmp_000525"
     CDS             38690..39550
                     /gene="pdxY"
                     /locus_tag="ID725_RS02625"
                     /old_locus_tag="pgaptmp_000525"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HGV7849203.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39594..40199)
                     /gene="gstA"
                     /locus_tag="ID725_RS02630"
                     /old_locus_tag="pgaptmp_000526"
     CDS             complement(39594..40199)
                     /gene="gstA"
                     /locus_tag="ID725_RS02630"
                     /old_locus_tag="pgaptmp_000526"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGV7849204.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40305..41807)
                     /gene="dtpA"
                     /locus_tag="ID725_RS02635"
                     /old_locus_tag="pgaptmp_000527"
     CDS             complement(40305..41807)
                     /gene="dtpA"
                     /locus_tag="ID725_RS02635"
                     /old_locus_tag="pgaptmp_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGV7849205.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQNGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42418..43053)
                     /gene="nth"
                     /locus_tag="ID725_RS02640"
                     /old_locus_tag="pgaptmp_000528"
     CDS             complement(42418..43053)
                     /gene="nth"
                     /locus_tag="ID725_RS02640"
                     /old_locus_tag="pgaptmp_000528"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGV7849206.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43053..43748)
                     /gene="rsxE"
                     /locus_tag="ID725_RS02645"
                     /old_locus_tag="pgaptmp_000529"
     CDS             complement(43053..43748)
                     /gene="rsxE"
                     /locus_tag="ID725_RS02645"
                     /old_locus_tag="pgaptmp_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGV7849207.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43752..44372)
                     /gene="rsxG"
                     /locus_tag="ID725_RS02650"
                     /old_locus_tag="pgaptmp_000530"
     CDS             complement(43752..44372)
                     /gene="rsxG"
                     /locus_tag="ID725_RS02650"
                     /old_locus_tag="pgaptmp_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGV7849208.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(44376..45434)
                     /gene="rsxD"
                     /locus_tag="ID725_RS02655"
                     /old_locus_tag="pgaptmp_000531"
     CDS             complement(44376..45434)
                     /gene="rsxD"
                     /locus_tag="ID725_RS02655"
                     /old_locus_tag="pgaptmp_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGV7849209.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45435..47753)
                     /gene="rsxC"
                     /locus_tag="ID725_RS02660"
                     /old_locus_tag="pgaptmp_000532"
     CDS             complement(45435..47753)
                     /gene="rsxC"
                     /locus_tag="ID725_RS02660"
                     /old_locus_tag="pgaptmp_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGV7849210.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDP
                     RKAAVEAAITRAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARKLEQQQANAEP
                     EEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
     gene            complement(47746..48324)
                     /gene="rsxB"
                     /locus_tag="ID725_RS02665"
                     /old_locus_tag="pgaptmp_000533"
     CDS             complement(47746..48324)
                     /gene="rsxB"
                     /locus_tag="ID725_RS02665"
                     /old_locus_tag="pgaptmp_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGV7849211.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48324..48905)
                     /gene="rsxA"
                     /locus_tag="ID725_RS02670"
                     /old_locus_tag="pgaptmp_000534"
     CDS             complement(48324..48905)
                     /gene="rsxA"
                     /locus_tag="ID725_RS02670"
                     /old_locus_tag="pgaptmp_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGV7849212.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48982..49422)
                     /gene="ydgK"
                     /locus_tag="ID725_RS02675"
                     /old_locus_tag="pgaptmp_000535"
     CDS             complement(48982..49422)
                     /gene="ydgK"
                     /locus_tag="ID725_RS02675"
                     /old_locus_tag="pgaptmp_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGV7849213.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49508..49723)
                     /gene="ydgT"
                     /locus_tag="ID725_RS02680"
                     /old_locus_tag="pgaptmp_000536"
     CDS             complement(49508..49723)
                     /gene="ydgT"
                     /locus_tag="ID725_RS02680"
                     /old_locus_tag="pgaptmp_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGV7849214.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(49996..50121)
                     /gene="blr"
                     /locus_tag="ID725_RS02685"
                     /old_locus_tag="pgaptmp_000537"
     CDS             complement(49996..50121)
                     /gene="blr"
                     /locus_tag="ID725_RS02685"
                     /old_locus_tag="pgaptmp_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGV7849215.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            50364..51404
                     /gene="ydgJ"
                     /locus_tag="ID725_RS02690"
                     /old_locus_tag="pgaptmp_000538"
     CDS             50364..51404
                     /gene="ydgJ"
                     /locus_tag="ID725_RS02690"
                     /old_locus_tag="pgaptmp_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGV7849216.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAIMLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51440..52441)
                     /gene="add"
                     /locus_tag="ID725_RS02695"
                     /old_locus_tag="pgaptmp_000539"
     CDS             complement(51440..52441)
                     /gene="add"
                     /locus_tag="ID725_RS02695"
                     /old_locus_tag="pgaptmp_000539"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGV7849217.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(52545..53717)
                     /gene="malY"
                     /locus_tag="ID725_RS02700"
                     /old_locus_tag="pgaptmp_000540"
     CDS             complement(52545..53717)
                     /gene="malY"
                     /locus_tag="ID725_RS02700"
                     /old_locus_tag="pgaptmp_000540"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGV7849218.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53727..55319)
                     /gene="malX"
                     /locus_tag="ID725_RS02705"
                     /old_locus_tag="pgaptmp_000541"
     CDS             complement(53727..55319)
                     /gene="malX"
                     /locus_tag="ID725_RS02705"
                     /old_locus_tag="pgaptmp_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGV7849219.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55494..56522
                     /gene="malI"
                     /locus_tag="ID725_RS02710"
                     /old_locus_tag="pgaptmp_000542"
     CDS             55494..56522
                     /gene="malI"
                     /locus_tag="ID725_RS02710"
                     /old_locus_tag="pgaptmp_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGV7849220.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLVHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56634..57401
                     /gene="hdhA"
                     /locus_tag="ID725_RS02715"
                     /old_locus_tag="pgaptmp_000543"
     CDS             56634..57401
                     /gene="hdhA"
                     /locus_tag="ID725_RS02715"
                     /old_locus_tag="pgaptmp_000543"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGV7849221.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57630..58220
                     /gene="uidR"
                     /locus_tag="ID725_RS02720"
                     /old_locus_tag="pgaptmp_000544"
     CDS             57630..58220
                     /gene="uidR"
                     /locus_tag="ID725_RS02720"
                     /old_locus_tag="pgaptmp_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGV7849222.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58611..60424
                     /gene="uidA"
                     /locus_tag="ID725_RS02725"
                     /old_locus_tag="pgaptmp_000545"
                     /pseudo
     CDS             58611..60424
                     /gene="uidA"
                     /locus_tag="ID725_RS02725"
                     /old_locus_tag="pgaptmp_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            60421..61794
                     /gene="uidB"
                     /locus_tag="ID725_RS02730"
                     /old_locus_tag="pgaptmp_000546"
     CDS             60421..61794
                     /gene="uidB"
                     /locus_tag="ID725_RS02730"
                     /old_locus_tag="pgaptmp_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGV7849223.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            61833..63097
                     /gene="uidC"
                     /locus_tag="ID725_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /pseudo
     CDS             61833..63097
                     /gene="uidC"
                     /locus_tag="ID725_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(63142..64650)
                     /gene="ydgA"
                     /locus_tag="ID725_RS02740"
                     /old_locus_tag="pgaptmp_000548"
     CDS             complement(63142..64650)
                     /gene="ydgA"
                     /locus_tag="ID725_RS02740"
                     /old_locus_tag="pgaptmp_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGV7849224.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLVKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(64751..65926)
                     /gene="manA"
                     /locus_tag="ID725_RS02745"
                     /old_locus_tag="pgaptmp_000549"
     CDS             complement(64751..65926)
                     /gene="manA"
                     /locus_tag="ID725_RS02745"
                     /old_locus_tag="pgaptmp_000549"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGV7849225.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            66125..67771
                     /gene="fumB"
                     /locus_tag="ID725_RS02750"
                     /old_locus_tag="pgaptmp_000550"
     CDS             66125..67771
                     /gene="fumB"
                     /locus_tag="ID725_RS02750"
                     /old_locus_tag="pgaptmp_000550"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGV7849226.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            67914..69317
                     /gene="fumC"
                     /locus_tag="ID725_RS02755"
                     /old_locus_tag="pgaptmp_000551"
     CDS             67914..69317
                     /gene="fumC"
                     /locus_tag="ID725_RS02755"
                     /old_locus_tag="pgaptmp_000551"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGV7849227.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69314..70243)
                     /gene="tus"
                     /locus_tag="ID725_RS02760"
                     /old_locus_tag="pgaptmp_000552"
     CDS             complement(69314..70243)
                     /gene="tus"
                     /locus_tag="ID725_RS02760"
                     /old_locus_tag="pgaptmp_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGV7849228.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70319..71620)
                     /gene="rstB"
                     /locus_tag="ID725_RS02765"
                     /old_locus_tag="pgaptmp_000553"
     CDS             complement(70319..71620)
                     /gene="rstB"
                     /locus_tag="ID725_RS02765"
                     /old_locus_tag="pgaptmp_000553"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGV7849229.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(71624..72343)
                     /gene="rstA"
                     /locus_tag="ID725_RS02770"
                     /old_locus_tag="pgaptmp_000554"
     CDS             complement(71624..72343)
                     /gene="rstA"
                     /locus_tag="ID725_RS02770"
                     /old_locus_tag="pgaptmp_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGV7849230.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72472..72807
                     /gene="ydgC"
                     /locus_tag="ID725_RS02775"
                     /old_locus_tag="pgaptmp_000555"
     CDS             72472..72807
                     /gene="ydgC"
                     /locus_tag="ID725_RS02775"
                     /old_locus_tag="pgaptmp_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGV7849231.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(72804..73526)
                     /gene="folM"
                     /locus_tag="ID725_RS02780"
                     /old_locus_tag="pgaptmp_000556"
     CDS             complement(72804..73526)
                     /gene="folM"
                     /locus_tag="ID725_RS02780"
                     /old_locus_tag="pgaptmp_000556"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGV7849232.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73563..74945)
                     /gene="ydgI"
                     /locus_tag="ID725_RS02785"
                     /old_locus_tag="pgaptmp_000557"
     CDS             complement(73563..74945)
                     /gene="ydgI"
                     /locus_tag="ID725_RS02785"
                     /old_locus_tag="pgaptmp_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGV7849233.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(75131..76075)
                     /gene="ydgH"
                     /locus_tag="ID725_RS02790"
                     /old_locus_tag="pgaptmp_000558"
     CDS             complement(75131..76075)
                     /gene="ydgH"
                     /locus_tag="ID725_RS02790"
                     /old_locus_tag="pgaptmp_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGV7849234.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            76599..78131
                     /gene="pntA"
                     /locus_tag="ID725_RS02795"
                     /old_locus_tag="pgaptmp_000559"
     CDS             76599..78131
                     /gene="pntA"
                     /locus_tag="ID725_RS02795"
                     /old_locus_tag="pgaptmp_000559"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGV7849235.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            78142..79530
                     /gene="pntB"
                     /locus_tag="ID725_RS02800"
                     /old_locus_tag="pgaptmp_000560"
     CDS             78142..79530
                     /gene="pntB"
                     /locus_tag="ID725_RS02800"
                     /old_locus_tag="pgaptmp_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGV7849236.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(79555..80589)
                     /gene="tqsA"
                     /locus_tag="ID725_RS02805"
                     /old_locus_tag="pgaptmp_000561"
     CDS             complement(79555..80589)
                     /gene="tqsA"
                     /locus_tag="ID725_RS02805"
                     /old_locus_tag="pgaptmp_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HGV7849237.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            81001..81366
                     /gene="mdtJ"
                     /locus_tag="ID725_RS02810"
                     /old_locus_tag="pgaptmp_000562"
     CDS             81001..81366
                     /gene="mdtJ"
                     /locus_tag="ID725_RS02810"
                     /old_locus_tag="pgaptmp_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGV7849238.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            81353..81682
                     /gene="mdtI"
                     /locus_tag="ID725_RS02815"
                     /old_locus_tag="pgaptmp_000563"
     CDS             81353..81682
                     /gene="mdtI"
                     /locus_tag="ID725_RS02815"
                     /old_locus_tag="pgaptmp_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGV7849239.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(81721..82542)
                     /gene="ydgD"
                     /locus_tag="ID725_RS02820"
                     /old_locus_tag="pgaptmp_000564"
     CDS             complement(81721..82542)
                     /gene="ydgD"
                     /locus_tag="ID725_RS02820"
                     /old_locus_tag="pgaptmp_000564"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGV7849240.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(82642..82725)
                     /locus_tag="ID725_RS02825"
                     /old_locus_tag="pgaptmp_000565"
     CDS             complement(82642..82725)
                     /locus_tag="ID725_RS02825"
                     /old_locus_tag="pgaptmp_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849241.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(82818..83126)
                     /gene="asr"
                     /locus_tag="ID725_RS02830"
                     /old_locus_tag="pgaptmp_000566"
     CDS             complement(82818..83126)
                     /gene="asr"
                     /locus_tag="ID725_RS02830"
                     /old_locus_tag="pgaptmp_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGV7849242.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            83397..83501
                     /locus_tag="ID725_RS02835"
                     /old_locus_tag="pgaptmp_000567"
     CDS             83397..83501
                     /locus_tag="ID725_RS02835"
                     /old_locus_tag="pgaptmp_000567"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGV7849243.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(83550..84803)
                     /gene="ynfM"
                     /locus_tag="ID725_RS02840"
                     /old_locus_tag="pgaptmp_000568"
     CDS             complement(83550..84803)
                     /gene="ynfM"
                     /locus_tag="ID725_RS02840"
                     /old_locus_tag="pgaptmp_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7849244.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            84910..85803
                     /gene="ynfL"
                     /locus_tag="ID725_RS02845"
                     /old_locus_tag="pgaptmp_000569"
     CDS             84910..85803
                     /gene="ynfL"
                     /locus_tag="ID725_RS02845"
                     /old_locus_tag="pgaptmp_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7849245.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            85938..87158
                     /gene="mlc"
                     /locus_tag="ID725_RS02850"
                     /old_locus_tag="pgaptmp_000570"
                     /gene_synonym="dgsA"
     CDS             85938..87158
                     /gene="mlc"
                     /locus_tag="ID725_RS02850"
                     /old_locus_tag="pgaptmp_000570"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGV7849246.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            87283..87978
                     /gene="bioD"
                     /locus_tag="ID725_RS02855"
                     /old_locus_tag="pgaptmp_000571"
     CDS             87283..87978
                     /gene="bioD"
                     /locus_tag="ID725_RS02855"
                     /old_locus_tag="pgaptmp_000571"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGV7849247.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(87931..89223)
                     /gene="clcB"
                     /locus_tag="ID725_RS02860"
                     /old_locus_tag="pgaptmp_000572"
     CDS             complement(87931..89223)
                     /gene="clcB"
                     /locus_tag="ID725_RS02860"
                     /old_locus_tag="pgaptmp_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGV7849248.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(89381..89995)
                     /gene="dmsD"
                     /locus_tag="ID725_RS02865"
                     /old_locus_tag="pgaptmp_000573"
     CDS             complement(89381..89995)
                     /gene="dmsD"
                     /locus_tag="ID725_RS02865"
                     /old_locus_tag="pgaptmp_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGV7849249.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(90038..90892)
                     /gene="ynfH"
                     /locus_tag="ID725_RS02870"
                     /old_locus_tag="pgaptmp_000574"
     CDS             complement(90038..90892)
                     /gene="ynfH"
                     /locus_tag="ID725_RS02870"
                     /old_locus_tag="pgaptmp_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGV7849250.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(90894..91511)
                     /gene="ynfG"
                     /locus_tag="ID725_RS02875"
                     /old_locus_tag="pgaptmp_000575"
     CDS             complement(90894..91511)
                     /gene="ynfG"
                     /locus_tag="ID725_RS02875"
                     /old_locus_tag="pgaptmp_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGV7849251.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(91522..93945)
                     /gene="ynfF"
                     /locus_tag="ID725_RS02880"
                     /old_locus_tag="pgaptmp_000576"
     CDS             complement(91522..93945)
                     /gene="ynfF"
                     /locus_tag="ID725_RS02880"
                     /old_locus_tag="pgaptmp_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGV7849252.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(94006..96432)
                     /gene="ynfE"
                     /locus_tag="ID725_RS02885"
                     /old_locus_tag="pgaptmp_000577"
     CDS             complement(94006..96432)
                     /gene="ynfE"
                     /locus_tag="ID725_RS02885"
                     /old_locus_tag="pgaptmp_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HGV7849253.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(96631..96936)
                     /gene="ynfD"
                     /locus_tag="ID725_RS02890"
                     /old_locus_tag="pgaptmp_000578"
     CDS             complement(96631..96936)
                     /gene="ynfD"
                     /locus_tag="ID725_RS02890"
                     /old_locus_tag="pgaptmp_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGV7849254.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            97044..97754
                     /gene="ynfC"
                     /locus_tag="ID725_RS02895"
                     /old_locus_tag="pgaptmp_000579"
     CDS             97044..97754
                     /gene="ynfC"
                     /locus_tag="ID725_RS02895"
                     /old_locus_tag="pgaptmp_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGV7849255.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(97757..98317)
                     /gene="speG"
                     /locus_tag="ID725_RS02900"
                     /old_locus_tag="pgaptmp_000580"
     CDS             complement(97757..98317)
                     /gene="speG"
                     /locus_tag="ID725_RS02900"
                     /old_locus_tag="pgaptmp_000580"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGV7849256.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(98352..98693)
                     /gene="ynfB"
                     /locus_tag="ID725_RS02905"
                     /old_locus_tag="pgaptmp_000581"
     CDS             complement(98352..98693)
                     /gene="ynfB"
                     /locus_tag="ID725_RS02905"
                     /old_locus_tag="pgaptmp_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGV7849257.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            98828..99154
                     /gene="ynfA"
                     /locus_tag="ID725_RS02910"
                     /old_locus_tag="pgaptmp_000582"
     CDS             98828..99154
                     /gene="ynfA"
                     /locus_tag="ID725_RS02910"
                     /old_locus_tag="pgaptmp_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGV7849258.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            99360..99446
                     /locus_tag="ID725_RS02915"
                     /old_locus_tag="pgaptmp_000583"
                     /pseudo
     CDS             99360..99446
                     /locus_tag="ID725_RS02915"
                     /old_locus_tag="pgaptmp_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(99921..100108)
                     /locus_tag="ID725_RS02920"
                     /old_locus_tag="pgaptmp_000584"
                     /pseudo
     CDS             complement(99921..100108)
                     /locus_tag="ID725_RS02920"
                     /old_locus_tag="pgaptmp_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(100143..100394)
                     /locus_tag="ID725_RS02925"
                     /old_locus_tag="pgaptmp_000585"
     CDS             complement(100143..100394)
                     /locus_tag="ID725_RS02925"
                     /old_locus_tag="pgaptmp_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGV7849259.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(100467..>101857)
                     /locus_tag="ID725_RS02930"
                     /old_locus_tag="pgaptmp_000586"
     CDS             complement(100467..>101857)
                     /locus_tag="ID725_RS02930"
                     /old_locus_tag="pgaptmp_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGV7849260.1"
                     /translation="DAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRA
                     NKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQPEPVKVTADEVKK
                     IMQAANISQPDAEELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQK
                     AEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIFD
                     GENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTS
                     RAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDTM
                     KWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTILRRS
                     YERQGSPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSA
                     IWQKLIPSQADS"
BASE COUNT        25466 a        25080 c        26321 g        24990 t
ORIGIN      
        1 cgggttaggg aaaatatctg gtgttgggga cagagtcagc agcgtattag ttgaatgctt
       61 aatatcaggc tgctgaattg tcagcactgt gtttgcgaag aggatgcgaa ataaatgaac
      121 ccggttgatc gtccactatt agatattggc ttaacgcgat tagaattctt gcgtatatca
      181 ggaaaaggcc ttgctggtct aacaattgcc cctgcgttac tgtcactttt gggttgcaaa
      241 caggaagata ttgatagcgg cacagtaggg ttgataaata cacccaaagg ggtgctggtg
      301 acccagcgag cgcgatgcac gggttgccac cgctgtgaaa tctcttgtac caacttcaac
      361 gatggctcag tagggacatt cttctcccgt atcaaaatcc atcgcaatta tttctttggc
      421 gacaacgggg ttggctctgg cggcggcctg tatggcgatc tcaactatac cgcggacacc
      481 tgccgtcaat gcaaagagcc gcaatgcatg aacgtctgcc cgattggtgc gattacctgg
      541 cagcagaaag aaggctgtat taccgtcgat cataaacgtt gtattggctg tagcgcctgt
      601 actacggcat gcccgtggat gatggccacc gtaaataccg aaagtaaaaa atcctcgaaa
      661 tgtgtgttat gcggtgaatg cgcaaacgcc tgcccaacag gggcgttaaa aattatcgag
      721 tggaaagata ttactgtgtg aatcttgcaa aggaaaaaat catgactaac ggttggacag
      781 gtaatatatt aagagtcaat ctcacgacag gaaatattac cctcgaagat tccagtaagt
      841 ttaaaagttt tgtcggtggc atgggcttcg gctacaaaat tatgtatgac gaagtaccgc
      901 caggcacgaa acctttcgat gaagcgaata aattagtctt tgctaccggc ccattaactg
      961 gatctggtgc cccctgtagt tctcgcgtaa atatcacctc actttctact tttaccaaag
     1021 gaaatttagt cgtcgatgcc catatgggtg gcttttttgc agcgcaaatg aaattcgctg
     1081 gatacgacgt cattattatc gaagggaagg cgaaatcacc ggtatggctg aagattaaag
     1141 atgacaaagt tagcctggaa aaagcagatt tcttatgggg aaaagggacg cgcgcaacga
     1201 cggaagaaat ttgtcgcttg accagtccgg aaacctgtgt ggcggctatt ggtcaggctg
     1261 gggaaaacct tgttcctctc tctggcatgt tgaatagccg taaccacagc ggcggtgcgg
     1321 gaactggcgc aataatgggt tcgaaaaacc tgaaagcgat tgcggttgaa gggacgaaag
     1381 gggtcaacat tgccgatcgt caggagatga agcgtctcaa tgattacatg atgactgaac
     1441 ttatcggtgc gaataacaac catgtcgtgc caagtacgcc gcaatcgtgg gcagagtatt
     1501 cagatcccaa gtcacgctgg acagcacgta aagggctgtt ttggggcgcg gctgaaggcg
     1561 gtccaattga aacgggtgaa attccgccag gcaatcagaa tacggtcggc tttcgtacct
     1621 ataaatccgt ttttgactta ggtccggcag cagagaaata cacagtaaaa atgagcggct
     1681 gccactcttg cccgatccgt tgtatgaccc aaatgaatat tcctcgggtg aaagagtttg
     1741 gcgtgcccag cacaggtggt aacacttgtg tagcaaactt tgtccatacc accatcttcc
     1801 cgaacgggcc gaaagatttt gaagataaag acgatggtcg tgtgattggt aacctggttg
     1861 gtctgaattt gttcgatgac tacggcctat ggtgtaacta cgggcagttg catcgcgact
     1921 ttacttattg ttacagcaaa ggtgtgttca agcgtgttct gcctgctgaa gagtatgcag
     1981 aaattcgctg ggatcaactg gaagcgggag acgttaactt cattaaagat ttttactacc
     2041 gtctggcgca tcgtgtgggt gagctgagtc acctggctga tggttcatat gccatcgcag
     2101 aatgctggaa tttgggtgaa gagtactggg gctacgcgaa aaataaactc tggtcgccgt
     2161 ttggctatcc ggttcaccat gccaatgaag cgtcagcgca ggtcggttcc attgttaact
     2221 gtatgttcaa ccgtgactgc atgacgcata cccatatcaa ctttattggt tccggcttgc
     2281 cattgaaact gcaacgtgaa gtggcgaaag aactttttgg ttctgaagat gcttacgatg
     2341 aaaccaaaaa ctacacgcca atcaatgacg caaaaattaa atatgctaag tggtcgctgt
     2401 tgcgggtctg tttgcataac gccgtcaccc tgtgcaactg ggtctggcca atgaccgttt
     2461 cgccgctgaa aagccgtaat taccggggcg atctggcgct tgaagccaaa ttcttcaaag
     2521 cgatcactgg cgaagagatg actcaggaaa aattagattt agctgcagag cgtatcttta
     2581 cattgcatcg tgcctacacg gtgaaactga tgcaaaccaa agatatgcgt aatgaacacg
     2641 atcttatctg ttcctgggta ttcgacaagg atccgcagat cccggtcttt actgaaggta
     2701 ctgacaaaat ggatcgtgat gatatgcatg cctcgctcac gatgttctac aaagaaatgg
     2761 gctgggaccc acagcttggt tgtccgaccc gcgaaacatt gcagcgtctg gggctggaag
     2821 atattgccgc cgatctggca gcacacaatc tactgcctgc gtaaggaatg ggtaagatga
     2881 accatcagga tgaattgccg ttagcgaaag tcagcgaagt tgacgaggcg aagcggcaat
     2941 ggttgcaggg gatgcgtcac cccgttgata cggtaaccga accagaaccg gctgaaatcc
     3001 tggcagagtt cattcgccag cattctgtgg ctgggcagct ggtggcgcgc gccgtgtttc
     3061 tctcgccacc ctattcagtt gcagaagagg aactgtcagt tttgctggaa aacatcaagc
     3121 agaacggaga ctacgcggat atcgcttgta tgacgggaag tcaggatgat tactattact
     3181 caacgcaggc catgagtgaa aactacgcgg cgatgtcttt gcaagttgtg gagcaggaca
     3241 tttgtcgcgc cattgcacac gccgttcgtt ttgagtgcca gacgtatcct cgtccttaca
     3301 aagtcgccat gctgatgcaa gcgccatatt acttccagga agcgcagatt gaagccgcga
     3361 tcgccgcaat ggatgttgca cctgaatatg ccgatatacg gcaggtagag tcatcgacgg
     3421 cggtgttgta cttgtttagc gagcgattta tgacctatgg caaggcttat ggcttatgcg
     3481 aatggttcga ggtagagcag tttcagaacc cctgaaattt cagggagatg tcatggatag
     3541 ggtggacagt tgcagccact gcattagggg ataatcagat gtccttcact cgacgcaaat
     3601 ttgttctggg gatgggaaca gtaatctttt ttactggttc tgcgtcttct ctgttagcga
     3661 acacgaggca agaaaaggaa gttcggtatg ccatgattca tgacgagtca cggtgcaatg
     3721 gctgtaatat ttgcgcctgc gcatgccgta aaactaacca cgcccccgct cagggaagcc
     3781 gtttatcgat agcacacatt ccagtcaccg ataacgacaa cgaaacgcag tatcactttt
     3841 ttcgccagtc atgtcagcac tgcgaagatg ctccgtgcat cgacgtatgc ccgacagggg
     3901 cgtcgtggcg tgatgagcag gggatcgtgc gggtagaaaa atcgcagtgc atcggctgca
     3961 gttattgcat tggcgcttgt ccgtaccagg ttcgctacct taaccctgtc actaaagtgg
     4021 cagataaatg tgatttctgc gctgagtccc gactggcaaa aggctttccg cccatttgcg
     4081 tcagcgcttg cccggaacac gcattgatct ttggtcgcga agatagccct gaaattcagg
     4141 cgtggttgca ggataataaa tactatcaat atcagctacc tggtgccggg aaaccgcatc
     4201 tgtatcgccg gttcggtcaa catttgatta aaaaggaaaa tgtatgaacc cgtcgcaaca
     4261 tgctgaacag tttcagagcc agttagcgaa ctatgtgcca cagttcactc ccgaattttg
     4321 gccggtgtgg ttgatcattg ccggagtatt gctggttggg atgtggttgg tgctggggct
     4381 gcacgccttg cttcgtgctc gtggcgtgaa gaaatcagcc accgatcatg gtgagaggat
     4441 ttatctttac agcaaagcgg tcagattatg gcactggtcg aatgcgttac tctttgtatt
     4501 gttgctggca agtgggctga taaatcactt tgcgctggtg ggcgcaactg cggttaaaag
     4561 tctggttgcg gtgcatgaag tttgcggatt tttgttactg gcatgctggc tcggctttgt
     4621 tctgatcaat ggcgttgggg gtaatggcca ccactatcgc attcgtcgtc agggttggct
     4681 ggagcgggcg gcaaaacaaa cgcgatttta tttgtttggc attatgcagg gggaagaaca
     4741 tcctttcccg gcaacaaccc agtctaaatt taatccctta cagcaggttg cctatgttgg
     4801 tgtcatgtat ggattgctgc cgttgttact attgacgggg ctgctgtgtc tctatccgca
     4861 agccgtggga gatgtgtttc ctggcgtaag atactggtta ttgcaggcgc attttgctct
     4921 ggcatttata agcctctttt ttatcttcgg tcatctttat ctttgcacca cggggcgtac
     4981 gccacacgaa acctttaaaa gcatggtcga tggctatcac cggcactaac gaatgatcat
     5041 tacacgagca gacctgcgag aatggcgtat cggagcggtg atgtatcgct ggttcctgcg
     5101 ccatttccca cgcggcggta gttatgccga tattcatcat gcactgattg aggaaggata
     5161 tacggactgg gcggagagtc tggttgagta tgcgtggaaa aaatggctgg cggatgaaaa
     5221 cttcgcgcat caggaagttt cctctatgca aaaactggcc acagaccccg gagagagagc
     5281 attttgcagt cagtttgccc gcagcgacga tcatgctcgc atcggctgtt gtgaggataa
     5341 cgccagaatc gcaaccgctg ggtatgctgc acaaattgcc agtatgggtt atagcgtgcg
     5401 gattggaagt gtcggcttta acagtcacat tggcagctcc ggtgagcgcg cacgggttgc
     5461 tgtaaccggc aattcttcgc ggataagcag cgctggagat agcagtcgga ttgcgaatac
     5521 cggaatgcga gtacgcgttt gtacattggg taaacggtgt catgtcgcca gtaatggtga
     5581 cctggtgcaa atcgccagct ttggcgcaaa cgccagaatt gccaacagcg gcgacaatgt
     5641 acacatcatt gccagcggtg aaaattcaac ggtagtgagc acgggcgtgg tcgactcaat
     5701 tattctcggg ccagggggca gcgccgcact ggcatatcat gacggcgaaa gagttcgctt
     5761 cgccgtcgcc attgaaggtg aaaacaatat tcgagccgga gtccgctatc gtcttaatga
     5821 acagcatcag tttgttgagt gctgatagct ttacctttaa tcaaacgaaa gacgccgacg
     5881 agcacgggat gcaggcttta cgaccgcccg agccatcgcc tcaccaattt ctgcacatgc
     5941 cgtaagtccc tgaacgaaag gctggtcgtg catcaaccag ggcaacgcgc cgaacgctac
     6001 gcgcccacga atatcttcgg gttgctgtaa cgtataatct tcaccaacat cagggatttc
     6061 atcccctgtt ttctgtaatt gttcgcgtag tccagggaaa ggaatatctt tcactttaag
     6121 cggacgctgc ccgcgggcat caataaaaac gtcaaacgag tagctgttgt cttccgtttt
     6181 caggacggtg cgcgactcgt taatttccat tttgtagtct tcaccgagag cgagaatatg
     6241 aatgattccc gcttcacgta aggcaagtag gcgacgaata gactctgaag ggattgccgc
     6301 ataattatcg atgaaaaccc gggcaaggcc tttactgaac cgtttatggt cgtgttcatt
     6361 cagatgtgga acaatttcct gtacggcttc atgcaggcgc agaatgacat aacgccaggg
     6421 aacagtatgt ttttctcgtt tattgcgttc gacttcctgg agattttttt ctgcccagtc
     6481 aaattggtcg cgttgtttgc gctcggcaaa ccaggcttga gcaaaggaat cgacattcag
     6541 gctctctaag gctatgcgtt gactccagtc tggatcagca aacttgattt cctctactat
     6601 caatctaaat acccgatcca aaaggccata ttcgcctttt tgaatctcag catttaatgc
     6661 ctgatcagtg acgatgtgta agggctcgta gggaataggg caatagaaat cggcttcggg
     6721 taaaataccc gtgcgcgaca ttaacgtaat atttagcttt tcacttgcgt tatcgcggtg
     6781 aaaaacgacg tgttgtttat catcttcaat gaacgaacca tgctgaatag ccactgccat
     6841 tgccgcatcc agtccgctca aggatgttcc cataataccc acgttacacg catcgacctt
     6901 tgcttccatc aagcctgacc acgggcttgg aaaatacgtt cgggttgctt cttcttcatc
     6961 aggccagacg tgacccgtgg cgatcaccgc taaatcaaac gtctcgctgg gtaaatcctg
     7021 atttgtagcg agcatgacgc cagcatttgt aatttgcaga tcggtaacct ggcatgattc
     7081 ataaacagcc actgcaaatt tttgctttcg tgcctggtct actaatcgta aaaattgatc
     7141 gcggaaatat tcgcccagca gaattcgcgg taaaaactga cgatcgtgca aggtttcttt
     7201 tttaacgcca taacgctgga gatgactggc ttcttgcttt tgtagccatt cgagatacgt
     7261 acaataaatc ggcggtattt caatactggc aatatttgcc agcatcattt ttgagttttc
     7321 ctcatcactg tatggcatcc cgacaccggc ctcgtcagcc tgctcgaaga tagaaataga
     7381 aagtagagtt tgttgctgta gaagcgagaa taaggtgtag atccccgtag gcccggcacc
     7441 cacaattgca atttttttca ttgttactta cctgtacgca ttctttcgaa tttagtgtta
     7501 tcagataagt ttggttgaga tttttagaat ttgagaaatt tcagacaggg atgaaaaggg
     7561 cgctaaccgc ccttgattaa attctgtcag gcgagttttg cctgattgat ttgactcagt
     7621 ggctcattga catcgaaaac tttggcgaca acttcctcaa cgccgagttt tttcagttgg
     7681 gcagtgtgtt tttccagata ggcaagggcg cttttttcat cagtgaaaag gtagatccca
     7741 ccggcttcgt ggttcttttc actttctgtc cataccttcc acagaaaacc aggttcctga
     7801 ttaatcgact ctgcaagtgg tttaagctgc tcagccattg cgtcgccaaa tgggccatta
     7861 aaagcaaaat gaagttgtaa caaagttgcc atagcgattc tccttctgat aacgcatacg
     7921 aagacagtgt aaatctttta acgtattgga tacagagaag aagcagaggg aaaaaggaga
     7981 agaccagatt aacaaaatct ggtgcgtccg agtggactcg aaccaccgac ccccaccatg
     8041 tcaaggtggt gctctaacca actgagctac ggacgcagga tggtgcgttc aattggactc
     8101 gaaccaacga cccccaccat gtcaaggtgg tgctctaacc aactgagcta tgaacgcaac
     8161 gttgtaggtg acaacgggga cgaatattag cggcagagtg ggaaggtggc aagaggcaaa
     8221 acgtaatttt ctgcgctatt tcgaccgttt gcagagcttt taagcaaatt ggctatattt
     8281 tgttgatttg caagggcgat ttttattcag gatcgcattt acatctgata caacccaata
     8341 attcgtgtat cagagtttaa acagagttgt ttgccaccat ctcaaatctg tcgtggataa
     8401 atcctccgta gcctttacac ttaggtagca ataaaggcac acagatgcag caaaggaata
     8461 aatcatggga tctgatgcga aaaacttgat gagcgacggg aatgtgcaaa ttgttaagac
     8521 cggcgaggtc attggcgcga cgcaacttac tgaaggcgag ttgattgttg aagctggcgg
     8581 aagagccgaa aataccgtgg tcacgggggc tggctggttg aaagtggcaa ccggtgggat
     8641 cgccaaatgc acacagtacg gtaacaatgg cacgctatcg gtcagcgacg gtgccattgc
     8701 cacagatatt gttcagtccg agggaggcgc aattagtctc tctacgctcg ctacggttaa
     8761 tggccgccat cccgaaggtg aattcagcgt tgatcagggt tatgcctgcg gtttgttgct
     8821 ggaaaatggc ggtaacctgc gtgtactgga aggacatcgc gcggaaaaaa tcattctcga
     8881 tcaagagggc ggcctgttgg ttaatgggac aacctcagcg gtcgtggtag atgaaggtgg
     8941 tgaattgttg gtgtatccag gtggggaagc cagcaattgt gagattaatc agggcggcgt
     9001 ttttatgctg gcggggaaag ccaatgatac gttgcttgct gatggcacca tgaataatct
     9061 cggtggtgaa gactctgaca ctattgttga gaatggagcc atctatcgtc tggggacgga
     9121 tggtcttcag ctctacagtt ccggtaagac gcaaaacctg tccgttaatg tgggtggtcg
     9181 ggctgaagtg catgccggta cgctggaaaa tgcggtaata caaggtggaa cagtgatcct
     9241 gttgtcaccc accagcgcgg acgaaaattt tgtcgtagag gaagatcgcg caccggttga
     9301 actgaccggg agtgttgcat tactggacgg cgcttcaatg attattggct atggcgcaga
     9361 tctgcaacaa tcaacgctta ctgtacagca gggcggtgta ttgattctcg acggcagtac
     9421 ggtaaaaggt gacagtgtca ctttcagtat tggtaacatc aatctgaatg gcggaaaact
     9481 gtggctgatc actggtgcgg caacgcatgt gcagctgaaa gtgaaacgcc tgcgcggaga
     9541 gggagcgatt tgcctgcaaa ccagtgcgaa agaaatttca cctgacttca tcaatgtgaa
     9601 aggggaagtt aacggggata tacgcgttca gataacagat gccagtcggc aaactctgtg
     9661 taacgcactg aaactacagc cagacgaaga cgggattggc gcaacgctcc agcctgcgta
     9721 aaaacacagg cgcaccccgt gaaggttgcg ccttgtcttt attagcggga tgctcgttgc
     9781 agaatgatgg ctgacggcag acgttgcagg taccgcatac gcagcatcat cataatggct
     9841 gccgacgtca ggccaataat aaagcctatc cagaagcctg ctggccccat aggttcaacg
     9901 accagatcgg tcagtgccag aatatagccg cttggcaagc ccagcaccca gtaagccgta
     9961 aaggtgatat agaaaatgga acgcgtatct ttataaccac gcaaaatccc actgccaatc
    10021 acctggattg agtcagaaat ctgatatacc gccgccagca acatcaaatg cgccgccagc
    10081 gttacaacct cgggattgtc gttgtacaac agggcgattt gctcccgcag tgaaaccgtg
    10141 aatatggccg tcagggttgc catacagaca cccaccataa gcccggtccg cgcagcggtt
    10201 tgcgcatcca gcgttgagcc ctgacccaga cgataaccta cgcggatagt taccgctgcc
    10261 gccagcgaca ttggaagcac gaacattagt gaactaaagt tcagggcaat ctggtgtcct
    10321 gcgacatcaa caataccgag cggagacact aacagagcca cgacggcaaa cagtgtcact
    10381 tcaaagaata gtgccagcgc aatcggcaaa ccgagttgaa tcagtcgttt cataaccgca
    10441 ggatcgggtt tcgcggtgcc tttttcgtta cgaatatcgc gcatcgagcg ggcgcgttta
    10501 atgtaagaaa ccatggcaag gaacatgacc caatacaccg ccgcagtagc cacgccacaa
    10561 ccaacgccac cgagctcagg cataccgaaa tgaccataaa taaagatata gttcaccgga
    10621 atgttcacca gcaggccgat aaagcccatt accatacccg gcttggtttt tgccagacct
    10681 tcacattggt tacgggcaac ctggaagaaa agatagcccg gcgcgcccca cagcaacgca
    10741 cgcagataac ccacggcttt gtccgccaga gccggatcga tgttttccat ggagcggata
    10801 atgtaacctg cattccacag caccagcata atgagaacgg aaacaaaacc cgccagccag
    10861 aagccctgtc gcacctgatg cgcaatgcgc tcacgtcgac cggaaccatt taattgcgcg
    10921 ataaccggcg ttaatgccag cagcagtccg tgaccaaaga ggatcgccgg aagccagata
    10981 gaagtaccga tagcgaccgc cgccatgtcg gtggcactat agccgcccgc catcacggta
    11041 tcgacaaaac ccatcgcagt ttgggcgatt tgcgcgagaa tcaccgggat tgctaatgct
    11101 aataacagac gcgcttcact gatatacttc tgcacgtgaa caccttttat ttgtagttat
    11161 atgagagact aaaaagccgc ctgaataggc agcaaaaaga agaagcaggg gaaattcagt
    11221 ctattgtagc gaggatttat taattctcca gtgaaaaaat agccattctt accccttcac
    11281 tggtaagggc gttttccacc tgctattgtg ctgaacagaa tgtcttaact gatttcagga
    11341 gttgtaagta tgtttacggg gattgtacag ggcaccgcaa aactggtgtc gattgacgag
    11401 aaaccaaatt ttcgcacaca tgtggtggag ttacccgacc acatgctgga cggtctggaa
    11461 accggtgcct ccgtggcgca taacggttgc tgcctgaccg tgacggaaat taacggcaac
    11521 catgtcagtt ttgacctgat gaaagaaacg ttgcgcatta ccaatcttgg cgatttaaaa
    11581 gtgggggatt gggtaaacgt tgagcgtgcg gcgaaattca gtgatgaaat tggcggacac
    11641 ttaatgtcag gtcatattat gaccaccgct gaagtggcga aaatattaac ctcggaaaat
    11701 aatcgccaga tctggtttaa agtccaggat agtcagttga tgaaatatat cctgtacaaa
    11761 ggatttattg gcatcgacgg tattagcctg accgtcggcg aagtcacgcc aacgcgtttt
    11821 tgcgtccatt taattccgga aactctggaa cgcacgactc ttgggaagaa aaaacttggc
    11881 gcacgcgtca acattgaaat cgatccacaa actcaggcag tggtagatac ggtagaacgt
    11941 gtgctggcgg cacgagaaaa tgccatgaat caatcaggta cagaagcctg atctgaaatc
    12001 gcccccggag agggggcgat agaatagcct ttagcgagcg actcgaaggc cgttttcaac
    12061 accgcgtgag aacaccacct gccagagctg aatatcacgg gcgcggaaag cacctgcgca
    12121 ggcattcaga taataggtaa acatccgttt aaagcgttca ctgtagttat ccgcaatttc
    12181 tggccatgcg gcgacgaatc gttcatacca cgccatcaac gtagtatcgt aatcagcacc
    12241 gaagttatgc cagtcttcca tcacaaagtg gggttcgctg gactgagcaa tctggcgtac
    12301 agagggcagg caaccgttcg ggaaaatata tttattaatc cagggatcaa cattcagatc
    12361 ggtttttttc gaaccgatag tatggagcag gaatatgcct tccggtttca aattacgatc
    12421 caccaccgta aaataggtat cgtaattttt cggtccgacg tgctcgaaca tccccacaga
    12481 aacaatacga tcaaactggt cgttcaggtc acgataatct tgcagcaaaa tggtgacatc
    12541 caggccttca cagcgttcct gagccatttt ttgctgttcg gcagaaatgg tgacgcccac
    12601 cacgcttacg tcataattag atgccatgta gtgtgccagt ccgccccagc cgcagccaat
    12661 atccagtacg cgcatcccag gttttaactg caatttttca caaatcattt tgagcttcgc
    12721 ctgctgggca gattccagat tatcggcatc tttccagtaa gcgcaggaat attgcatgaa
    12781 gggatcaagc atgcggctga acaagtcatt acccaaatcg taatgctctt tgccgactat
    12841 ccaggcacgt tttttactct gcagattgaa gagacgggcg ccggcaatac gcagcgtgtc
    12901 tttgaaatga tgggggagtt ggttctcgag acctgcgcgt aagactttgc taaaaaacat
    12961 atccagtcgg tcacattccc accagccatc catataactt tcgcctaacc ccaaagagcc
    13021 ttcttgcaga acgcgtttaa aaaaatcggg gtttttcaca cgaatatccg ccggggcaga
    13081 accgttaatg gctataccgg cacggctaag taattcgttg gcgatacggt accagttgtc
    13141 atccggtaca ctgacttctt ctatacacga tgaactcata gtttctccat cactggtcgg
    13201 tgatcagaac cttaaaacag cgtagacgct ttttgggctt tgtgagaaat ctcgcagaaa
    13261 aaaccgcgag tctataatcc gacgtagaac agagggaaag gaggaaccct tgccgaaatg
    13321 accatccata gtgagtcggg aacgatcctg tccccgtaaa aacataatat ttatcgtagc
    13381 tttaacaact cgaaattagt ataggccgct aatttcaatc atacaacaaa attttgttag
    13441 cagcctaagt ataagtatat cgatatagat cagtgtgatt cgctatgagc gacttcggca
    13501 ttgccatgat tctggcagcc aacttcttca caacgttgca tcatgtaacc cagcgcgacc
    13561 agcactactg ttgataacat cacgctggtg gtggtgagca atggcgtgct gatactgatc
    13621 agccaggaaa ctaccagact tgcgaggaag cacagaccca gttgcagagt gttctgcaac
    13681 gctgcggcgc gaccagttgc gtgtgggaag ggacgcagag cctgggcgac aacaataggg
    13741 tagatcgcgc cattagcaat cgccatcaca cagaatggga tcaggatttc gaccagcgac
    13801 acatggctaa tgaagcccgc agcccaggtc gcaatgacgc tgacagcaaa cagcaccagc
    13861 aaccacggta ataactgctt gccttgccat ttctgcagcg cggcgcgaca gccataacca
    13921 ccaatcagaa acgcgatagt ttgcgggaca taacttaaac caataactgc cgggctgtag
    13981 cccatttcac taaggatgaa cggtgaaccg gtcagccatg caaaaaaact ggctgaacag
    14041 gccgcgtata tcagcacatt gccgcgatag gttttagaac gtagcaggtc ggtaaaggtc
    14101 agaccatcct gactattgtt acgggccttc gtcgtgggtt tgagccagaa aataggcaga
    14161 atcagcacca cggtaatggc aaaaagggtg gcgaaaatcg cctgccagga aaaatggacc
    14221 agcagccagc ttcctaacag aggagccagg gccggagata gacccaccag cggcatgatg
    14281 gtcgcaaaaa ttcggttaac tttctgtgaa ggataataat ccgtcactaa cgcttgccag
    14341 ataaccgccg cggcgcagac acccacagcc tgtacaaaac gcaataccag cagcatagcg
    14401 gcgttttcta cccacagcat ccccagacta cctaacgcaa aaattgtcag gccgattaat
    14461 aataccggtt tacgaccata acgatcggag agcggccccc acagaagctg ggctgcggca
    14521 aaaccggcaa ggaacagact aaggctggca ctgacagcag acgcaggcgt ttgcaggtcg
    14581 gcctgtatgg cggcgaaagc aggcagatac atatcggttg ccagaaaacc gagtacgctc
    14641 aaacccgcca gccagactaa aaatcttttc ccaggttgca tgtatattct ctaaatctct
    14701 caattaaaca ccgccgcaaa gtgtagggag tgaaattagt gttgtgaaac ggtaatattt
    14761 gatgactggt ttcaaaaatt ttgcaggcag actatgtggt cagaatattc actcgaagtt
    14821 gttgatgcgg tagcgcgtaa tggtagtttt agcgctgcgg cacaggagct gcatcgcgtt
    14881 ccttctgcgg tcagctatac cgtgcgtcag ctggaagagt ggctggcggt gccgctgttt
    14941 gaacgacggc accgtgatgt ggagctgacc gctgccggag cgtggtttct caaagaaggg
    15001 cgctctgttg tcaaaaaaat gcagatcacc cgccagcaat gtcagcagat agcgaacggc
    15061 tggcgcggtc agttagctat cgcagtggat aatattgtca ggccagaacg tacacggcag
    15121 atgatcgttg atttttatcg ccattttgat gatgtcgaac ttcttgtctt tcaggaagtg
    15181 ttcaacggtg tctgggatgc gctttccgac gggcgcgtgg aactggcgat tggcgcaaca
    15241 cgcgcgattc cggtaggcgg tcgttatgcc ttccgggata tggggatgct aagctggagt
    15301 tgcgttgttg ccagccatca cccgctggcg ttgatggatg gcccgttcag cgatgatacg
    15361 ttgcgcaact ggccgtcgtt ggtgcgcgaa gacacctcgc gaacgctacc taagcgtatt
    15421 acctggttgc tggataacca aaaaagagtt gtcgtgcctg actgggaatc atcggcaacc
    15481 tgtatctcgg caggattatg catagggatg gtgccaacac atttcgccaa accgtggctt
    15541 aatgagggga agtgggtagc attagaactg gaaaacccct ttccggattc ggcatgttgt
    15601 ctgacatggc agcaaaatga tatgtcgcct gcgttaacct ggttgcttga gtatctcggc
    15661 gatagtgaga cgctgaataa ggagtggctg cgggagccgg aagagactcc cgcaacgggt
    15721 gattaacgac gatagtcgcg gaacgggccg tcagccacgg agcggcgttc aatcaagcgc
    15781 ggatgcactt caatggactg cggttcttca cgtttgttga ctatacgatc caacaacatg
    15841 ttgaacgctg tttcacccaa cgaatctttt ggctgatgga ttgttgtcag cgccggcgta
    15901 aaatagcggg cgttgcgcac gttatcataa ccgatcagcg aaacatcctg cgggacgcgc
    15961 agccccattt catcagcagc acaaagtgcg cccattgcca tgatatcgcc accacagaag
    16021 acggcagtag ggcgatgcgg ctgcgacagg atttgctgca tggcgcgata accggattca
    16081 ggttcaaagt caccctgcac aatccagctt tccggcacct tgatcattgc ttcttccatc
    16141 gccttcataa aaccggcaag gcggcctgcg ccagtgttac gttccagcgg gccggggata
    16201 acgccgattt cgcggtgacc gcgttcaatc agataacgcc cggccatgta gccgccttcg
    16261 aacgcgttat caatgaccgc atcggtgaag tcagcttttg cttcacccca gtccatcacc
    16321 accattggga tatggcgata ctcttccagc atcgccagca acggctctgg gtactcagaa
    16381 cacatcacca gcagaccatc gacgcgtttt tgcgccatca tcgacagata agcccgctgt
    16441 ttctcaagat tgttccacgc attacccaga atcagggtgt aacctttctg gaagcaattt
    16501 ttttcaactg cttcaatgat ctcggcaaaa taggccgctt cgctgctggt cgctagcaaa
    16561 ccgatagact tggtgtggtt aaccttcagg ctacgcgcca ccgcgctagg ggagtagtgt
    16621 aattctttga tcgctgccca cacggcgttg cgcgtttctt cagcgacgaa acgtgttttg
    16681 ttgatcacgt gtgacacagt tgtagtggaa acgtttgctc gtttcgctac atcttttatt
    16741 gttgccattc catttcactc cagaccctaa cttcagctcc tgcaaaatcg caaggtaaac
    16801 gtttgccttt acacacctta cgcacaacaa aagtgttgcg gtacgccgga aaaacgacac
    16861 ggagcgtcag gaaggggtca atggccagta cgcaaatagt gtaagcgtgg aattttggcc
    16921 gatcttgacg aaaaggggaa gtggtaaaaa cacttatcat tataaatgac gggagatttt
    16981 ttcatcacag tgtgtaaaaa tgcgtaatat cactttttgt gtggtaataa aaaaggagaa
    17041 aacttgatga gcaccgacct taaattttca ctggtaacaa cgattatcgt cctcggtttg
    17101 atcgtggccg tgggtttgac tgccgcgctg cactgatttc ctctcgaggg agcgcgttct
    17161 ccctcgcatc tccttcctgt ttgttagcaa aatggcaaaa gtgttttctc tttttgttat
    17221 tttcattttt ttgtgattgc tgtcatgctt tcgccacttt aatgaatatc gcgtaatgac
    17281 gcgaagaggt ggagataagg catgaaaatt aactatccgt tgctggcgct ggcgattggc
    17341 gcgtttggta tcgggacaac ggagttctcg ccaatgggct tgttgcccgt cattgcgcgc
    17401 ggtgtggatg tctcgattcc cgctgccgga atgttaatca gtgcctatgc agttggcgta
    17461 atggttggcg cgccgctgat gacgcttcta ctttctcatc gtgcccgccg cagtgcgttg
    17521 attttcctga tggcaatttt cacgctcggc aacgtacttt ccgccatcgc gccggattat
    17581 atgaccctga tgctttcacg cattttgacc agcctgaatc acggagcatt ttttggtttg
    17641 ggttcagtcg tggccgcaag cgtggtgcca aaacataaac aggccagcgc agttgccact
    17701 atgtttatgg ggttaaccct ggcaaatatc ggtggcgtgc cggcggcgac ctggttgggt
    17761 gaaaccatcg gctggcggat gtcatttctg gcaacggcgg ggctgggagt gatttcaatg
    17821 gtaagtctgt tcttctcatt acctaaaggt ggtgcagggg cacgacctga agtgaaaaaa
    17881 gagctggcgg tattaatgcg tccgcaggtg ctgtctgcat tgctgacgac ggtactggga
    17941 gctggtgcaa tgtttactct ctacacctat atctctccgg tactgcaaag tattacccac
    18001 gcaacaccgg tgttcgtcac ggcaatgctg gtgctgattg gtgtcggatt ctctatcggt
    18061 aactatctcg gcggcaaact ggcagatcgt tcagttaacg gcacgttgaa aggctttttg
    18121 ttgctgctga tggtgattat gctggcaatc ccgttcctgg cccgcaataa gttcggcgca
    18181 gctattagca tggcggtgtg gggcgctgca acctttgcgg tcgtaccgcc gttacagatg
    18241 cgcgtgatgc gtgtcgccag tgaagcgcca ggtctgtctt catcagtcaa tattggtgcc
    18301 tttaatcttg gaaatgcgct gggagcagct gctggtggtg cggtaatttc cgctgggctg
    18361 ggatacagct ttgtgccggt gatgggggcg attgtcgcgg gactggcatt attgctggtg
    18421 tttatgtcag ccagaaaaca acctgaaaca gtttgcgttg ctaacagcta attaaacaaa
    18481 aacgcagaag gataaccttc tgcgttacag ttgctgacaa cgtgcgattt atcttgccga
    18541 atgcgctgtt aacgcctcat ccgcgttggc atggtaagcg aagcgcatca ggcaatactg
    18601 catttgccat cagttattat gcagcgagat ttttcgctac gaattcccag ttcaccagcg
    18661 cccagaagtg ctcaagatag ccaggacgtg cattgcgata gtcgatgtaa tatgcgtgtt
    18721 cccagacatc aacggttagc agcggagtcg catcggtggt cagcggagta cccgcgttag
    18781 aggttgaaac gatagccagt ttgccatcgc tgtttttcac cagccaggtc cagccagaac
    18841 caaagttttt gatcgctgca tcagtaaact gagctttgaa atcggcaaag ctgccaaaag
    18901 atgcggcgat agcttcagcg acttttccag tcggttcgcc accggcgttc ggtgccaggc
    18961 agttccagta gaaagtatgg ttccagacct gagctgcgtt gttgaatacg ccaccttcag
    19021 agctgcgaat aatctcttcc agtgatttac cttcaaacgc ggtaccttta atcaggttgt
    19081 tcaggttagt gacataagtc tgatgatgct tgccgtagtg atactcgata gtttccgcag
    19141 aaatgtgcgg tgccagagca tctttagcat atggtagtgc aggtaattcg aatgacattg
    19201 ctactctcct ttattattaa tttgcatacg tacaatagcc ttattgtgcg tatggatagg
    19261 gtagcaaatt aaaaggtgag acaaaagatg aacttaccct gttgccgtaa caacagggta
    19321 aagttttagc gaagtgtttt tggggtcatt acccggcgag cgccaacata gtggcgctgc
    19381 cagtagtctt cactgagaga agtgatttga atttcctgac ctgtgcgcgg tgactgaata
    19441 aatttgccgt tgccgacata cacgccgaca tgatcggctg taccgcgtcc ctgagtacgg
    19501 aaaaagacca ggtcgccgtt tttcagttca ctacgttcga ttggcgctgc atcgcgcagg
    19561 tgatacattt cattcgccgt acgcggaata cgaattttca ccaaatcttt ataagcgtaa
    19621 taaaccaggc cgctgcaatc aaaaccggta cgcggtgagc tgccacccca acgatatggc
    19681 ttaccaattt gctgcatcag tttattcatt gccacttttg tggctttttg caccttcgct
    19741 ttgtgtgcgt cggcaagggt tcctgacgct gcatttttga ctttcacaca atgcgattta
    19801 tagcccttac gtttggtaca cttttcggta aagctggctg aggccgtttt attgacggta
    19861 tgtttgctgc gagttttggc gtttttagaa gcggtaatgg aagatttttt aacggaagaa
    19921 gcggttttgc ttttggtcgt acttttactg gcggcttttt tcgctgtttt ctgggtcttt
    19981 ttggtggttg ccgtgctttt ctttttatct gcttttttgg tgatatgagt ggtagcagaa
    20041 ctctccctgg cttgctttga agcatgggcc ataggcgtta aaggcagggt ggtaaaaagt
    20101 aaagcacaga gcgtgatcga aatacggttt atccgcgcca ctgagcgtcc ctctgttaag
    20161 gcaggtctat catgcctgcg tctgatttac aaaacttgat gattctaatg catagaagaa
    20221 gagaaagtta atagctttta ttgttaaaaa atacgtttcg ttggcgagcg caaaaaaatg
    20281 cagcagtgaa agctaaaaat ggtcataaat acgccagttt gccgtgcatc agggaagaaa
    20341 cgaatatttg aaatgcaaca aatagagcaa agggataaaa tagcaaaagc gtgatgaaaa
    20401 tgttattttc cgatgccgca ctgacccgct acaatgtcag attactgccg taaagaagtt
    20461 tgaggaagca agacaatgag caccactatc gaaaaaatcc aacgccagat tgctgaaaac
    20521 ccgatcctgc tgtacatgaa aggttcaccg aaactgccga gctgcggttt ctctgcccag
    20581 gcagtccagg cgcttgccgc atgtggcgaa cgttttgcct atgttgatat tctgcagaat
    20641 ccggacattc gtgcggagct gccgaaatat gctaactggc cgaccttccc gcaactgtgg
    20701 gttgacggcg agctggtagg cggttgtgat atcgtgatcg aaatgtatca gcgtggcgaa
    20761 ctgcagcagc tgatcaaaga aactgccgct aaatacaagt ctgaagagcc ggacgcggaa
    20821 taatccgtcc tgcaatactg acggatgcga acgggcatcc gtcagtcctt tctctatccc
    20881 caatccagcc cttgtggcga acttgaaaat cccgcttcac gcagcagcgt aaacatcggc
    20941 gtttgccaga ccggtagatc attcacttct gttagcgtaa agcgcagccg tggttcgcga
    21001 cgcagtgcgg tagtcagcgc gtggaaaacc tctggggcga gtaattcctc tttttcctgc
    21061 cagaccagca tttttttgcc accttgcgcc agatagagta acaatttgcc gccagaaacg
    21121 accaccagcg cacccgcccg acgcgttggt accagcgttg ccggatgtgc aggccagggc
    21181 agaagatttc cccacacatt agccggatcg ttggcggaga gcgccactgg tgtatagtgg
    21241 cgcgtttgcg cggcttgtgt cgccaggtcg cgcaagcggt caatagtcag acgttcagcg
    21301 aattgcgcgc cacccagacc ttctacaaaa cgacctcgca taattcgccc ggaatcttcc
    21361 atacttcgac aaagcgtttg catcgatgga aacccgccag ggatattttc ggctatcacc
    21421 gcctgacgac tgatgatgcc gtagcggtcg agcatatttt ccgccagcgc cagcatcctt
    21481 tcggtatcgt ttagtggctc cacctgcaat aacgaccagc gtccagccag atttggtgtg
    21541 ttgtaagata cccgcggcga gacgggttgt gcatagacag gacgcccacg acgagcccgg
    21601 tgactgcggc gagttgaggt gcgtgcgttg gaactgctgc gggtgagggc gcgtaacggt
    21661 gcccaaatat cgctggtgat gacgccttgc cagaccagcg cccataacgc ctcttgcagt
    21721 gccgagggat caaccgattc gccgattttg tcgcgtatcc gttggctgat ttgttgtgca
    21781 aaccaggctc ctccgtcagc cagaacagcg actatcgctt gttgcagcgc cgaacgattc
    21841 gcctgatccg cttcggcggg agtgaacgat tctgcagcat attcctgtag atgcagtgcc
    21901 accaggccgt catcttcacc cagttttttt tgccccgacc agataaccgc accggttgcc
    21961 agtaattcat cgagcacttc tggcgaatag tcgtgtacgc gggcaggcaa gatctggctt
    22021 tcccagagtg aggcgggtaa gccgactccg gcaagctgtt cgatcacccg catcacgcca
    22081 tccaccccct cataaacgcc tggcgatgtt gaggcaaaga gcgccgggct accatcggtg
    22141 gcgggtaata cgccctgacg ttccagcagc aatcgcgcat aggtcgtggc tgcaacggga
    22201 cgcgtcgctt ctctggcggc ttgcagcgag cgcaaacgca gacgacgaaa tacttcatcg
    22261 ctcacccaga tgtcttgttg cagattcatc accagaccct gttcgcgcag ttgctgaagc
    22321 tgctcttcga caatggcaat accgagacta aactcatgag ccagttgttc agccgtgacc
    22381 agagcatgag cccggagata gcgtagaaag aggtcgcgaa gcgggtaact gactctatgc
    22441 aaataaatct ctggcagtga ctctggtaat cgtacgccga gggcatcgcg cagcctggcg
    22501 gcatcatcca tacaggcaag acgctcctgt ccactaatca tcgtcgggaa gatgcgtttt
    22561 actgtaagaa gattttccag atacgaggca atctcttcac tgctgcctgt atgccgttgc
    22621 gccaggtctt caacggtcat tggccccagt tcgcgcagca ggtcgaacaa tccttcttca
    22681 cctttcgctc ttctgccagg agccagtcgt tgcaactctt cttccacctg gcgaatgacc
    22741 tgcgggtcga gtaattcccc cggatcgacc tgtccgagta gattgcgcag taactcgctg
    22801 tccagcgaca gtacggatgc ccggcgttct gccagcgggg cgtcgctctg gtacataaat
    22861 tccgcgacat agccgaacaa taaacttgtg gcgaaaggcg agggcgtagt agtcgttaca
    22921 tcggatattt gaatttcgcc tccgttcagg cgacgcatca aacgttccag tgcgggaaga
    22981 tcataaacat cttgcagaca ttcgcgtagg gtttcgagaa tggccggaaa atccggatat
    23041 ccctgagcga tttccagcaa ctgactggcg cgcagccgtt gttgccaaag cggggtgcga
    23101 tggcccggcg tgcggcccgg cattaataat gcccgcgcgg cgcattcgcg aaaacgggcg
    23161 gcgaaaagtg ccgaactgcc taccgcctcg cggacaattt gcagcaactt ttctggttca
    23221 aacaaaaaaa tcgcggcgtc gggcaattta ccatcagtgt caggaatgcg cgcaacaatg
    23281 ccgtcatcac tggcgaccac agacgcgtca gcgccccaaa gcgcatgtat tcgcccggca
    23341 atcgccagcg cccagggttc atgcacccgt cttccatagg gagagtgcaa aataatacgc
    23401 caatcgccaa tttcatcacg gcaccgttcg aggaccagat gacgactccc cggaacaata
    23461 cccgtcgcgt tgcgctgctc gtcaatcaac ccctgaatat tggcgatcgt attttcttca
    23521 gccagccacg ggggaatagt cccggaaaag aacgctccat ccgccagcaa atgaagaaaa
    23581 tcgccgatca tctcgcctaa ttcagccgga cgtccgttac cttcaccacg ccagaagggg
    23641 agccgggcag aacgacccgg agcaggagtc acaatcacct gatcgcgggt gatttgctgg
    23701 atccgccatg aggttgcgcc gagagtgata atatcgttca cccgcgactc atataccatc
    23761 tcctcatcca gttcacccac ccgtcgcgaa ccggcctgtt cttccccttc gggtaataac
    23821 acgctataca tgccacgatc cggaatggtg ccgccgctgg taaccgccaa cagctgagcg
    23881 ccaggtcggg cggtcaatat cccggtctcc ctgttccaga ccagtttggg gcgaaaagca
    23941 gaaaaatcgc cagagggata gcgtccggaa agcatatcca gcgtggcgtc aaaaacacgt
    24001 cttggcagat ctttccacgg tgcggcacgg cgtacgcggg agtaccattc gtctacctgt
    24061 aatgcatcca tcgccgcggc ggcaacggtg tgctgcgcaa ggacgtcgag tggattatgc
    24121 ggaggtgtca ggttttccag cctgcctgcg aacatacact ctacaatgac tgcggaatcg
    24181 actaagtcac gcctggttcg ggggaaaaaa agccctttgg agatacctcc tatctggtgt
    24241 ccggcacgtc caatgcgttg taacccactg gcaacagaaa gcggcgttgc cacctgaatc
    24301 accagctcca cagcgcccat atcaatcccc agttcgagac tggaagttgc gaccacgcag
    24361 cgtaactcac ccgatttcag cgcctgctcg gtgattgctc gttgttcttt agagacggag
    24421 ccgtggtgtg agcgggcaat aaaaacgtcg ctactttgta cacggttaga ggttgcgcca
    24481 gaggtcgact cgaaatgggc cgcatcagcg gcgatagacg gggaacgctg taagcgtgcg
    24541 gcgtaaagct catttaatcg tgccgtcagt ttttccgcca gcccccgcga attagtaaag
    24601 acaatggtcg agcgatggcg caacacttca tcaaggatac ccgtttcaat atatggccag
    24661 atggagcctt cccggccggc atggctgtct tcgccagtgc cgctggcgac cgatgagaca
    24721 tcatccatat tggcgactgg tacgacaatc cgtatctgcg gatgacgcat tgcgggcggg
    24781 ttgactaccg taaccgggcg atcgccacca agaaatgctg ccacatcgct ggctgagcgc
    24841 acagtggcag aaaggccaat tcgctgtgct gaggtgtgga gcagcgcatc gagccgctcc
    24901 agacttaacg ccagatgcgc accacgttta cttcccgcta ccgcgtggac ttcatcaata
    24961 attaccgttt cgacgccgcg tagcgtttcg cgcgcgcggg aggtcagcat cagatagagt
    25021 gattcgggtg tggtgatcag aatatccggc ggattacggg tgagtttgct gcgttcctgt
    25081 gcaggcgtat cgccagtacg gatccccacg cgaagattga cttccgtttc gccgcgccgc
    25141 cgccgttcat cggcaatacc cttcaacggg atctgcaagt tgcgctgaac gtcggtgccc
    25201 agggctttta tcggtgaaat atagaggatg cgtgaggttt tacgtttatg cgcctcgcgg
    25261 gtatcttcgc cgccttcgcg gaagagtcga tcgagggcgt agagaaatgc tgccagcgtt
    25321 ttcccggagc cagtcggtgc aatcaccagc gcatgttcgc ctcgcgccgc cacatgccag
    25381 gtttgcgact ggacagcggt cggctgttta aaggcgcgaa gaaaccagtc gcgggtcgcc
    25441 ggtgaaaaca cgtccggcag gggcgatgaa gggtttggat tatctgccat taacgctatt
    25501 ccccgtgaat atcatgcatg atgcgtggaa tagcctcact atagatgcct gaatcattgc
    25561 atgtcatcag gcatcgactc gattacacct cttcggcggc aggtagcggc cagcctccca
    25621 gacgtttcca gcggttgacg atttcacaaa acagcaccgc agtgcgttcg gtgtcgtaca
    25681 gcgcggagtg cgcctgggtg ctgtcgaagt ccatgccagc ggtctggcaa gcctttgaca
    25741 atacggtttg tccgagtgcc agcccggcca gtgcagcagt gtcaaaagtg gcgaaagggt
    25801 ggaacgggtt acgtttcagt gaggcgcgtt ctgcggcggc catcataaag ctgtgatcga
    25861 aattggcgtt gtgcgccacc ataatggcgc ggttacagcc gctcgcttta atacctttac
    25921 gtacaacttt aaaaatttcg tgcagcgcct cgtattcgct gaccgcgccg cgatcgggat
    25981 cgttcgggtc aatgccgttg aaagcaaggg cttctggttg caaatttgcg ccgacaaatg
    26041 gttcgacgtg gaaatgtaat gtggtgtccg gcatcagcca gccttgttca tccattttca
    26101 gggtgatggc ggcaatctca agcagcgcat cggttttggc gttaaatcca gctgtttcaa
    26161 catcgatcac aacaggataa aagccacgaa aacggtcgca cagaccggta agttgagcgt
    26221 tatcggacat ctgattctct taatacggat aaattgcagc gcgcattatg acaaatatta
    26281 cttgtcgatg cagtaaagat gcgggcgcga tgagttcgcg cccggcagga gattagttgc
    26341 ccagaccgcg accggcgtct ttctcttcga ttaactcaat tttgtaaccg tccggatctt
    26401 ccacaaacgc gataaccgta gtaccgcctt ttaccggacc cgcttcacgg gtcacgttac
    26461 ccccgttttg acggattttt tcgcacgctt cagcggcatt atctacgcta agcgcgatgt
    26521 gaccataagc agtgccgagt tcgtatttat ccacgcccca gttgtaggtc agttcaatca
    26581 ccgcttcttc ggtttccggg ccgtagccaa caaacgccag tgagtatttg tattccgggt
    26641 tttcgctggt acgcagcagt ttcatgccca gcactttggt ataaaaatcg atggagcgtt
    26701 gcagatcgcc aacgcgcagc atggtatgaa gaagacgcat ctttttatcc tcaaaatggc
    26761 taacagattc aaaatgttgt tttagtatag cggcggcttt acgccgctcg caatgttgga
    26821 ttacaacgtc gggtaatcag tgtagccttc cgcaccgcca ccatagaaac tttcagcacg
    26881 ctgtgggtta agctcagctt tgcgctgcaa gcgggcgacc agatccgggt tcgcaatcca
    26941 gtcacgacca aatgccaccg catcaattaa ccctttgccg atcagcgttt cagctttttc
    27001 tactgtgtat gcacctgcgc cgataatcgg accgtggaaa cgggcgcgta ctttttcgcg
    27061 gaacgcatca gtatacggtt caccccccgc ccaatctggt tctgacatat gcagataagc
    27121 aatgccgcgt ttacccagtt gttcaatcag atacagtgca tcggcttctt cattcgggcc
    27181 gttatccgtg ttctggaaag taccgattgg cgaaacgcga atgccaatgc gatcggcacc
    27241 ccattcttca atcccggcat cgaccacttc cagtaccaaa cgtgcgcgat tttccacgct
    27301 gccgccgtac tgatcggtac gatggtttga agaaggagaa aggaactgat gcagcaaata
    27361 accatgagca gagtggagct ctaccagatc aaaaccggct tcacgcgcgt tagcaatagc
    27421 ctgacggaaa tcattgacga tacttggaat ctcttccagt tcaagcgcac gcggcatgga
    27481 tgtttcaaca cggatcgcct gaccattttc atcgcgcaga gaagtacgtg ttcccgcgct
    27541 aagtgctgaa ggcgctactg gtgcctggcc gccaggttgc aggctggcgt gagaaatgcg
    27601 tccggtgtgc cacagctgta cggccatatg cccattttca gcatgaacgc cagcggtaat
    27661 ttttttccat gcggcaattt gttccggact atggatgcca ggtgcaccag catacccttt
    27721 tgcctgggca gaaatttgcg tggcttcgct aataatcaaa ccggcactgg cacgttggcg
    27781 atagtattcc gccatcaacg gggtagggat gtcacccggt tcaatactgc gcagacgcgt
    27841 cagcggtgcc ataaaaatac ggtttgccgc agttattgcg cccactttca gtggggaata
    27901 cagtttttca gatgacataa aggctcccgg tcgtctggtg ataaaaaatg cctgctaaac
    27961 ggcaggcgtc gcaataatgg tttttacatg tgccagcgcg ttttccagtg gctcgtaatt
    28021 gcgcgaaatt ttggcctgca gattcgcacc cagccatagt gcgtaaagca cttgtgcctg
    28081 ttgcagcggt tcgccacaaa aggttaaaca atggctatca cggccatttt ccagagcctg
    28141 cgagagcagg gcgatcacgc cgcgagcgcc tttatccatc gcgctacgca tatcttctga
    28201 cagatcgcac acttcggcag agagttttac tgtcaggcaa ccactgatgg ttccatgttg
    28261 gcaaaactgg ttcagtgttt gctggtaata agccagtatg cggtcgcggt agttaccttc
    28321 gccggattgc agcaactcag tcagtcgttg gtgatatgtg gcgtaatggc gctcaagcat
    28381 ggcaacgcca aacgcttctt tagagcgaaa gtagtgatag aaggaccctt tcggcacttc
    28441 agcggttttt agtaattcgc ttagccccat cccggtgaat ccacgttgca ggcaaagttg
    28501 ctcgcccgtc gccaggagat gttcgcgagt atcatgttcg gtgtgtttgt tcatgcgttg
    28561 gagtgtagta gaccagtcgg tctagtgcaa gcaaagttgc tactttgggc attaacagct
    28621 tcaataatgg taataatctt atttgaggag ggcgaagtgg cggagcaatt agagttcttt
    28681 cccgtccaga gcccgtgccg gggaatttgc cagtctgacg aacgcgggtt ttgtcgcggc
    28741 tgttttcgaa gccgggatga gcgttttaac tggaataaaa tgagcgatgg cgaaaaacaa
    28801 gaggtgctgc gcctttgtcg gcagcgatta atgcgcaaat tacgtgcaaa taagccagcg
    28861 tcatcagacg aaccggagca accatcactc ttttgagcct aatcttgcgt atactcctct
    28921 catatctgat aagaggaagc gattatggtt cagcgtatta ctattgcgcc gcaaggcccg
    28981 gagttttccc gttttgtgat gggctactgg cgattgatgg actggaacat gtccgcccgc
    29041 cagctggtca gttttattga agagcatctg gatctcggcg tgaccaccgt ggaccatgct
    29101 gatatttatg gtggctatca gtgcgaagcg gcgtttggcg aggcgctgaa actggcacct
    29161 cacctgcgtg agcggatgga aatcgtcagt aaatgcggta tcgcgacgac cgcgcgtgaa
    29221 gaaaacgtca ttggtcatta catcactgac cgcgatcaca tcattaagag cgccgaacag
    29281 tcgctaatta atcttgcgac cgatcatctg gatctgctgt taatccaccg cccggacccg
    29341 ttaatggatg ccgatgaagt ggcggacgcg ttcaaacatc tgcatcagag cggcaaagtg
    29401 cgtcattttg gcgtatccaa cttcacgcct gcgcaatttg ccctgttgca atcacgtctg
    29461 ccgtttaccc ttgccactaa tcaggtggaa atatccccgg tacatcagcc gttactgctg
    29521 gatggcacgc tcgaccaact gcaacaactg cgtgttcgtc cgatggcgtg gtcctgcctt
    29581 ggtggtggtc gtctgtttaa tgatgattat ttccagccgc tgcgtgatga actggctgtg
    29641 gtggcggagg agttaaacgc gggctcgatt gaacaggtgg tttacgcctg ggtattacgt
    29701 ttaccgtcgc agccgctgcc aattatcggc tgaggtaaaa ttgagcgcgt acgggcagct
    29761 gtcgaagcag aaacactgaa aatgacccgt caacaatggt ttcgtatccg taaagcggca
    29821 ctggggtacg acgtaccgta agcactttta ggaatagccg ccgttcaaaa atgtgtcact
    29881 ggtttacact tattcaggaa tgcacaatga acggaggtcc tatgaaacgt tttagtctgg
    29941 ccattctggc gctggttgtt gcgaccggcg cacaagctgc cagtgaaaaa gtcgagatga
    30001 acctcgtcac gtcgcaaggg gttgggcagt caattggtag cgtcaccatt actgaaaccg
    30061 ataaaggtct ggagttttcg cccgatctga aagcattacc tcccggtgaa catggcttcc
    30121 atattcatgc caaaggaagc tgccagccag ccaccaaaga tggcaaagcc agcgccgcgg
    30181 aatccgcagg cgggcatctt gatccacaaa ataccggtaa acatgaaggg ccagaaggtg
    30241 ccgggcattt aggcgatctg cctgcactgg tcgtcaataa tgacggcaaa gctaccgatg
    30301 ccgtcatcgc gcctcgtctg aaatcactgg atgaaatcaa agacaaagcg ctgatggtcc
    30361 acgttggcgg cgataatatg tccgatcaac ctaaaccgct gggcggtggc ggtgaacgct
    30421 atgcctgtgg tgtaattaag taattaagag gccagcgtac cttgcggtgg tgcttgctca
    30481 agttgcgaaa gcgagcagta cagccgccag acaattgccg ccagctcacg ggcggcaggt
    30541 tgatgatggc gggcaaggct gtcgcaaatc cgctgtaatt cttcaagtgt ggccgccagt
    30601 gatttttgct gaacgccacg ctcactcatc actccccgca acagtgaaat acagttatcc
    30661 cgtactcgcg ataacggatc ggaacgcgat tcccagtcgc gtaattgcca gacaacatga
    30721 gaacagttca gcagcactat accccagcgt aataaccaac ggcgagccag cgcatcctgg
    30781 ctgttactca actgactgac gtgatgataa gtgagcgatt caaattcgct ttcactcagt
    30841 gttggatggc ggcttagttg atcgacaaaa tcccggcgca gcgcgcgaat atggcggcgg
    30901 cttttgcgag catccgatcc tggacgcaga atggcgaacg ctaaccacgc caacgcgacg
    30961 ccaacgattt ttgccagatt atcgttaaga aaatcagcaa aattatacac cgggggatta
    31021 gtgacggcga taaaagaacc cataaaaaca atcagttgcc cccacaatgc ggcaaatttg
    31081 ggcatctgca atttaagaag ctgcattgtc gccagcagtg gaaagagaaa cagtaaaaat
    31141 tgccacagat cgctaatctg gaccatcaga ccaaatttga ccacaaagct gaatagcgaa
    31201 agtaacacca gcgtgcgcat cagcagtgac aacgacttaa acggtgctgc gacggcggag
    31261 tagagtacgc agctaatcgc tgccagcgtt aatgcattgg caccggcatc ccattgcgaa
    31321 gcaatactcc atgcgccaat catcatcaac gtacaaaatg tacgcagtcc gctccacata
    31381 gcttcggcgt tatcggtatg acgagccagg ccggatgtcc gtgggagtct ggcatgatca
    31441 tctacatcgc tttgcagacg atgtaattcc tgactacttt gcagataaag gcggcaaaaa
    31501 taacgtagtc gctgccagaa ggcgacgtgc cgatagtcgg cgacgttggt cgggcgtagc
    31561 ggggcgataa tacgtgcgac ggtgtaaaca tctgtttgcg aactggcgag cgccgtcagc
    31621 aactgttcga gaatttctcg cgtggcacct ggcggtgagg gccagttgag caacatacgt
    31681 cgcaggctgg agatgacact ggtcatacgt aattgctggt ggagcagcgc attaaggcgc
    31741 gcgttttgct ggcgaaaacg atagtggctc cagaaagcct ggatacgcag caaattcatg
    31801 gtcagtatct gcccaatcac cccttcatgt gctgcacgaa tggcatcggt tgtttcaggc
    31861 tgccagagta aactggcatg ttccagtaat cgggcgtgca tgtttttcaa tgcggttaaa
    31921 agggctgtag catcggaact gctaggcagg atcatcatca tcatgccgcc gcacaaaata
    31981 ccgacaatca cctcgcaaac gcgcgcctga gcgatatccc acagctggct ggcctcagta
    32041 atattaacca tcggaaaggc gatgatggca gccgtgtagc ccgccagttg aaatgcatac
    32101 gcgacgttat tcgtgaagtg cgcacaggcc caggtacaaa agccaagcca cgccgacatg
    32161 ctcaatagaa aaaaccacgg ctcattgagc gtatgcccgg caagaagcag tgccgcaatg
    32221 gctcccagta aactgccagc gatgcgtccg aggcttttgc tgatgacacc gccaacggtg
    32281 ggaaagctaa ccactgcagc cgaggtcatc gcccaatagg gttcatccag attcaaataa
    32341 taggcaaccg tcagcgccag acacatggca atggtattgc gtaacgcata acgccattgc
    32401 gccagcgtgg ccctgaacca gggcaaattg cgcaaggacc atgacgatgc gttcattatt
    32461 gacctacggc aatgctacag gtggtgcccg acaccagcgt gacatcgcct ggaactttat
    32521 ccagcgcaaa gcggacggga acgcgttggg cgagacgaac ccaggggacg ttaggtttca
    32581 catccggaat aaggcttgag tcactttcaa cgctctgatc ataaatcgcc cgaccgatac
    32641 tggaaacgtg accctgtaac gttttgttat cactatagag ggtaatttgt gctggtgcac
    32701 cttcgcggat atggcgcaac ttggtttctt caaaataacc aatgacgtaa aacgagtggc
    32761 tgtcgacaag ggcaaacagc ggtttcccgg tgtcagcgta atcgccgatg cgggtagtga
    32821 gattagtcac ccatccactc accggggcgc gaatttccgt ttgcgccagt tgccattgtg
    32881 tctgtttcag cgtcgcctgt gcggcatcaa cactggcctg catcgcttta acattgaggt
    32941 tagcggtatc gagttcttcg gcagaaataa aattttgtga gagatgacga cgacgattcg
    33001 cttcgttatt ggcttttgcg aggtcagatt gcgctttcgc cagctgagcc tgtgcattca
    33061 gctcggcgat ctgaaagggc gttttatcga tcgtgagcag aagatccccc gcgttaacca
    33121 gctggttgtc ttttatattc agctcaacaa tacgtcccga cacctgtggg gtgatagaaa
    33181 cctgttctgc gcgtattttc ccgtcgcgtg tccacggaga ttgcatgtaa taattccaca
    33241 gccaccatcc ggcaagaacc gcaactaccg ctacaatgat tgttgagaaa tacttaattg
    33301 ttttaattga catagttacc acgcaatcag tattgccaga gcaaggcaaa cgcaaatcgc
    33361 aaacagcgat aaatccatta acaagggatg ccagatgtca ccggcgtaga tccagccacg
    33421 aagcaggcgg tgtacgacaa gccagatgac gaatccaaac gcgaaggctt tgaaaaacgg
    33481 aggaaagtag acggacgcac cgaacaccag atcttgtaag ggcaatcctg ttgcattgag
    33541 cataaacttc acaaagtaga ttcctttacg accgaatata ttgcgttaga ttaataaata
    33601 ttctttaact gcgaaaaatt tacgcgcaat ttctgaaaaa tatgtctgac ggtaaccaaa
    33661 tgcagcaata catttgtttt agcaatacaa ttgctgcaca ctattctaaa agccgcataa
    33721 tatcttagca agctaattat aaggagatga aattggaatc gccactaggt tctgatctgg
    33781 cacggttggt gcgcatatgg cgtgctctga tagaccatcg cctgaaaccg ctggagttaa
    33841 cacaaaccca ttgggttacg ttacacaata tccatcagtt acctccagac cagtcgcaaa
    33901 ttcaactggc aaaagcgatt ggcatcgagc agccatcact ggtccgtact ctggaccaac
    33961 tggaagaaaa agggttaatt tcgcgtcaaa cttgtgccag cgatcgtcgg gctaaacgta
    34021 ttaaactgac ggaaaaggca gagccgctga tcagcgaaat ggaagctgtt attaacaaaa
    34081 cccgcgcgga aattttacat ggcatctccg cagaggaact ggaacaactg attacgctca
    34141 tcgcaaaact tgagcataat atcattgagt tacaggccaa agggtgaaat gaagggggcg
    34201 tgtggtcagt aacctgacca cacgcaaact tatttagcgc ggagaaacgg tcacctgact
    34261 gccattgctg gccagtacga cacgttggcc cggagagaaa cgagtgttgc cttgtttctg
    34321 taccaccatg atggtattac catcgtcttt acgaatttcc agctcgacac cttgcgtttt
    34381 gttcattgca ctctgtacgc cctgaccagc tacgccacct gcaacagcgc ctgctgcagt
    34441 agccagagaa cgcccggttc cgccaccaac agtatttccc aggaaaccac caagaacagc
    34501 accgccaatt gcaccgataa cgttggaatc atcaccgccc tgaatctgta ccggacgtac
    34561 gttaacgatg gttccatagc tgacattctg tacttgtttc gcttcagaag cggtataaac
    34621 atcccctgac agggtgtcgt tattaacaca accgacaaga gacagaccta ccattgaaac
    34681 aaccaatacg cgtttaatca ttgaaaaatc tcctgttcac catgaaacgc cacgcaagca
    34741 tccctcatgg tcaaagtata tggcatatgt gaatccatgg ttacatgttc taccaaatat
    34801 gagcaaatca taatgaaagt aggatcaacc aattataaac aaaacattca tgatgattat
    34861 tgatttgaag ataatgtttt actaccaacg gtaaaaatcg gtcattccgc atggtattca
    34921 cgaaaagttt atgtttgagt gtgcgcaaac gtcagattga aaaaggatga actatgaaat
    34981 cgggccgctt tattggcgtt atgtcaggca ccagccttga tggtgttgat gttgtgttgg
    35041 cgacaattga tgaacaccgg gtcgcacagc tggcaagttt gagctggccg atcccggtgt
    35101 ctctgaaaca ggctgtactg gatatttgcc agggccagca gcttacactt tcgcagtttg
    35161 gacagcttga tactcaactc gggcgacttt ttgctgatgc ggtcaaggcc ttgcttaagg
    35221 aacaaaacct gcaggcgaga gatatagttg cgatcggctg tcacggtcaa accgtctggc
    35281 atgaaccgac gggcgtggca ccacacactt tacagattgg cgataacaat caaattgtgg
    35341 cacgcaccgg aattacggtt gtcggtgatt ttcgccgtcg cgatattgcc ttgggaggac
    35401 aaggcgcacc gctggtacct gcgttccatc atgccttgct ggctcaccca accgagcgac
    35461 gaatggtgct caatattggc ggcatcgcca atctgtcact gctcattcct gggcagccgg
    35521 ttggcggcta cgataccggt cctggtaaca tgctgatgga tgcctggatc tggcgtcagg
    35581 ccggtaaacc ttacgataaa gatgccgagt gggcacgggc gggtaaagtt attctcccac
    35641 tgctgcaaaa tatgctcagc gacccatatt tctcgcaacc tgcaccgaaa agcaccggac
    35701 gcgaatactt taactacggc tggctggagc gccatttacg ccattttccg ggtgttgatc
    35761 cccgtgatgt tcaggccaca ctggcagaac tcaccgccgt gaccatttct gaacaagttt
    35821 tgttgagcgg tggctgcgaa cgattgatgg tatgtggtgg aggtagtcgt aatccgctac
    35881 tcatggcgcg tctggcggca ttactgccag gcacagaagt caccaccacc gatgccgttg
    35941 gcattagtgg cgatgacatg gaagcattgg ctttcgcttg gcttgcctgg cggacgctgg
    36001 cgggattacc aggaaatctg ccttccgtca ctggcgcaag ccaggagacg gttctggggg
    36061 ctattttccc cgccaactcg tgatatcaga attaactgaa ttttttctta tcgtgtctgc
    36121 cgttacactt ggaagattaa ggaggatatt tgccctccgg accaggaaag tcttcgggat
    36181 atgactatga aaaaactgtt aattatcatt ttgccggtcc ttctctccgg ctgtagcgcc
    36241 tttaatcaac tggttgagcg aatgcaaacc gatacgctgg aataccagtg tgatgaaaaa
    36301 ccgttgacgg tcaaactgaa taatccgcgc caggaagtca gttttgttta cgataatcaa
    36361 ctactgcatc tcaaacaggg cctttcagcc tctggcgcgc gttactccga cgggatctat
    36421 gttttctggt cgaaaggtga ggaagcgact gtctataaac gcgaccgcat cgtcttgaat
    36481 aactgtcagt tacaaaatcc acagcgttga gatttttcca ggggcggcgc acaatagcgt
    36541 cacccacaga caatccgtaa agaaaaccat gtctgataac gacgaattgc agcaaatcgc
    36601 gcatctgcgc cgtgaataca ccaaaggcgg gttacgccgc cgcgatcttc ccgccgatcc
    36661 attaaccctt tttgaacgtt ggctctctca ggcttgtgaa gccaaactgg cggaccctac
    36721 cgcgatggtg gtcgctaccg tggatgaaca tggtcagcct tatcagcgca tcgttttact
    36781 caaacattac gacgaaaaag gcatggtgtt ttacaccaac ctcggtagcc gtaaagcaca
    36841 tcaaattgaa aataatccgc gcgttagcct gctgttcccg tggcataccc ttgaacgcca
    36901 ggtgatggtg atcggtaaag cagaacgact ttcgactctc gaagtgatga aatattttca
    36961 tagccgcccg cgtgatagcc agattggttc atgggtttcg aagcagtcca gtcgcatttc
    37021 tgcccgcggt atccttgaaa gtaaattcct ggagctgaag cagaagtttc aacaaggcga
    37081 agtgccattg ccgagctttt ggggcggttt tcgcgtcagc cttgaacaga ttgagttctg
    37141 gcagggcggt gagcatcgcc tgcatgaccg ctttttgtac cagcgtgaaa atgatgcgtg
    37201 gaagattgat cgtcttgcac cctgaaaaga tgcaaaaatc ttgctttaat cgctggtact
    37261 cctgattctg gcactttatt ctatgtctct ttcgcatctg gcgaaaagtc gtgtaccggc
    37321 aaaggtgcag tcgttatata catggagatt ttgatggcaa gcagtaactt gattaaacaa
    37381 ttgcaagagc gggggctggt agcccaggtg acggacgagg aagcgttagc agagcgactg
    37441 gcgcaaggcc cgatcgcgct ctattgcggc ttcgatccta ccgctgacag cttgcatttg
    37501 gggcatcttg ttccattgtt atgcctgaaa cgcttccagc aggcgggcca caagccggtt
    37561 gcgctggtag gcggcgcgac gggtctgatt ggcgacccga gcttcaaagc tgccgagcgt
    37621 aagctgaaca ccgaagaaac tgttcaggag tgggtggaca aaatccgcaa gcaggttgcc
    37681 ccgttcctcg atttcgactg tggagaaaac tctgctatcg cggcgaacaa ctatgactgg
    37741 ttcggcaata tgaatgtgct gaccttcctg cgcgatattg gcaaacactt ctccgttaac
    37801 cagatgatca acaaagaagc ggttaagcag cgtctcaacc gtgaagatca ggggatttcg
    37861 ttcactgagt tttcctacaa cctgttgcag ggttatgact tcgcctgtct gaacaaacag
    37921 tacggtgtgg tgctgcaaat tggtggttct gatcagtggg gtaacatcac ttctggtatc
    37981 gacctgaccc gtcgtctgca tcagaatcag gtgtttggcc tgaccgttcc gctgatcacc
    38041 aaagcagatg gcaccaaatt tggtaaaact gaaggcggcg cagtctggtt ggatccgaag
    38101 aaaaccagcc cgtacaaatt ctaccagttc tggatcaaca ctgcggatgc cgacgtttac
    38161 cgcttcctga agttcttcac ctttatgagc attgaagaga tcaacgccct ggaagaagaa
    38221 gataaaaaca gcggtaaagc accgcgcgcc cagtatgtac tggcggagca ggtgactcgt
    38281 ctggttcacg gtgaagaagg tttacaggcg gcaaaacgta ttaccgaatg cctgttcagc
    38341 ggttctttga gtgcgctgag tgaagcggac ttcgaacagc tggcgcagga cggcgtaccg
    38401 atggttgaga tggaaaaggg cgcagacctg atgcaggcgc tggtcgattc tgaactgcaa
    38461 ccttcccgtg gtcaggcgcg taaaactatc gcctccaatg ccatcaccat taacggtgaa
    38521 aaacagtccg atcctgaata cttctttaaa gaagaagatc gtctgtttgg tcgttttacc
    38581 ttactgcgtc gcggcaaaaa gaattactgt ctgatttgct ggaaataatg cattaagtgg
    38641 aaagggggag tgagaaatca ctccccctgg tttttataca gggaacatga tgaaaaatat
    38701 tctcgctatc cagtctcacg ttgtttatgg tcatgcgggt aacagtgcgg cagagtttcc
    38761 gatgcgccgc ctgggcgcga acgtctggcc gctgaacacc gttcaatttt ctaatcacac
    38821 ccaatacggc aaatggactg gctgcgtgat gccgcccagc catttaaccg aaattgtgca
    38881 aggcattgcc gccattgata aattacacac ctgtgatgcc gtattaagtg gctatctggg
    38941 atcggcggag cagggtgaac atatcctcgg tatcgtccgt caggtgaaag cagcgaatcc
    39001 gcaggcgaaa tatttttgcg atccggtaat gggtcatcca gaaaaaggct gtatcgttgc
    39061 accgggtgtc gcagagtttc atgtgcggca cggtttgcct gccagcgata tcattgcgcc
    39121 gaatcttgtt gagctggaaa tactctgtga gcatccggta aataacgtcg aagaagcggt
    39181 tctggcagcg cgcgaactca ttgcgcaagg gccacaaatt gtattggtta aacacctggc
    39241 gcgagctggc tacagccgtg accgttttga aatgttgctg gtcaccgccg atgaagcctg
    39301 gcatatcagc cgtccactgg tggattttgg tatgcgccag ccggtaggtg ttggtgatgt
    39361 gacgagcggt ttactgctgg tgaaactgct tcagggggca acgctgcagg aggccctgga
    39421 acatgtgacc gctgcagtct acgaaatcat ggtgaccacc aaagcaatgc aggaatatga
    39481 gttgcaagtg gttgctgctc aggatcgtat tgccaaacca gaacattact tcagcgcaac
    39541 aaagctctga aatatctcat ttcggcccac aacgaactgt gggccgacgg ctattacttt
    39601 aagccttccg ctgacagcgc gtcttgtact tccggacgtt cagccatacg ttgcataaat
    39661 gccgcaatgt gctctaaccc ttccagattc agtttcaccg cgtatgccca gcgcagtacc
    39721 gtaaacagat aggcatcagc aattgtaaat ctttgcccgc agatccagtg ctcatccttc
    39781 agtgcctcgt tcacatattg cagtttcttc tccagctgcg cgcgaactgt cggtttgtac
    39841 tcttccggtg tatcagggcg aaacagaggt gtgaaacctt tatgcagctc ggtggcgatg
    39901 taattcagcc attcgatggt tttatagcgg gaaatactgt ttaccggtgc cagcaactgg
    39961 cggtcgggga cgctgtcggc aagatactgc ataatcgcta caccttccgt cagcaaagta
    40021 ccgtcatcca gcagcaatgc aggtacctgt cccttagggt taacggcaaa gtaatcgtca
    40081 ccgttttcga gacgtttttt cattaaatcc acactgacga gcgtaaaatc ctttccgctc
    40141 tcacgcaggg tgatatggga agcgagagag caggcgcccg gtttgtagaa caatttcatc
    40201 ggtaactcct tttgtctgtg gtttaaagta tggtagtgcg ttgctgaaca aaaaaaaagc
    40261 cgctaacgaa aagttagcgg cttgtaaaag agtttccctg aattttacgc tacggctgct
    40321 ttcgccgctt tgtctgcagc gtcatcctgc gtcatgcggt gcagtttcgg cgcggtcagc
    40381 agcatcagta ctgcaataac ggcagtagcg acaccaatct gcaagaatac gcgaccatag
    40441 acttccagtg acatcagcgg atcggtaacg ttatccggca cagccatcat acccgcaaca
    40501 taaccaccaa tcaggtttgc accggcagtg gtcaggaacc agctacccat aatgaagccc
    40561 atcagacgct gcggaacgag ttgtgcaacc attgccagac ccagaccaga gatcatcagt
    40621 tccccgatac tctgcaggcc atagctcgcg accagccagc ttacagacac gatacctgcg
    40681 tcagacgcga atttcgcacc cagcggcaga atcaggaacg caccagaaca catcaccata
    40741 ccgattgcaa acttggttgg catcggcagg gtatcgccca tcttgttata gatagcggcc
    40801 agaatcgggc taccaatgat gatccagaac gggttcagtg cctgatactg ctcaggttct
    40861 acggccaggc ccagaatggt atgttcaacg ttacgaatcg caaagaagtt cagtgacgtt
    40921 ggcatctggc tgtacagcac gaagaagata atggcttcga gcatcaggat gaaggcaacg
    40981 atcattttac gacgcgcagc acctttcatc gcgaaggctt ctttaccgaa gataaccacg
    41041 ataccgaagg caacaacgcc cagcgccata cgcgcaactt cctgattgtg cagcagccag
    41101 gtggcgatag cgatcagtgc cacaacacca ataatggtca gcagcaggtt acggtagttg
    41161 ataggctcga agtctggttt tgaaccgttc tgtttaaccc agcgttggca gaaggcgaag
    41221 ttaacgatag tgatcagcag gcctacaacg ctcaacgcaa acgcaacact ccagccgtat
    41281 ttcgcggcca gccacggggt ggcgatcata gagaagaaag agccgatgtt gacggacatg
    41341 tagtacatgg tgaatgcacc gtccagacgc gggtcgtttt tctcatagca tgtagaaagc
    41401 agagaagacg ggttagcttt aaacaggccg ttaccgaccg caatagccgc catacccata
    41461 taaacgatac cggcgtcgtg accagaccag gcaaccagag cataaccaat cgccagcaca
    41521 atagcgccga gcataattac gcgtttagta cccagtacct tgtcacctaa ccagccgccg
    41581 atagcgacca gaccataaac cagggcacta aaggaagaga aaagggtgat tgaatccgct
    41641 tcagacatac ccagttgttt aaccaggtaa acagccataa ttccttgtag gccgtaataa
    41701 ccaaaacgtt cccataactc aatcgagaag atgagataga acgccttcgg ttgtttgaaa
    41761 gcgttcaaac tgacgctttc agttggtttt tggtttgcag tggacacttt tacctctttt
    41821 tttacatccc atattaacgg gggtgctttc tcgcgttatg tctaaaaaac atacgcgcaa
    41881 ttgttatagt gggaggggaa acggcaggta atgttcacta tcttgcactt tcagacaagt
    41941 cctttgtaat aatctgttac atataactga ggcggggaat tcaaccattc cgggaaatgt
    42001 tatccagcgt taaatttttc aaaaaacgta acggcagatt ttttcactgc attgagaggg
    42061 ggatgatggc ttgacggcga tatgttctgc tatttagcgt ttttcctagt gagatagtcc
    42121 agtttcttaa aaatagccag tgtaatgttt tgtaggtcaa tatttgttgc gtgtgtaaat
    42181 ttaaatgatt ttaacctgat tcttgcaatg tttttacatt gccacagcat cgtgatcttg
    42241 atcactaatg aatagaaaat ttcgctatta aaaaaacaat taacctgcat ttttggttat
    42301 ccgcaaacgg attacctgat gataacgcta agcaatggca tcattgacgt taaaaagatg
    42361 ttttaaaaga ggataaagaa aggttatcaa tggggtaatc ggtgttaccc cttttcttca
    42421 gatgtcaact ttctctttgt attcacaaag atcttcaata atacaagagc cacagcgggg
    42481 cttgcgggca atgcaggtat aacgcccgtg caggatcaac caatggtggc agtcgacttt
    42541 aaactctgcc ggaaccactt tcagtagctt ttcttctacc tgttcgacgt ttttccccgg
    42601 cgcaaattga gtacgattac aaacgcggaa aatgtgcgtg tcgacagcaa tagtcggcca
    42661 gccgaatgca gtgtttaata cgacgttggc tgttttacga cctacgccgg gcagggcttc
    42721 aagcgcagca cgatcttccg gaacctcgct attatgctgc tccagtaaga tacggcaggt
    42781 tttgatgata ttttctgctt tgctgttata aagcccaatc gttttgatat aggttttcac
    42841 cccttcaacg cccagttcaa gcatcgctgc aggcgtattc gccaccgggt agagtttcgc
    42901 cgtcgcctta ttaacactga catcggttgc ctgagcggaa agcagtacgg caatcagcaa
    42961 ttcaaaaggc gaactgaaat taagctcagt ggtgggatga ggattgttct cacgcaggcg
    43021 agtgaggatc tccaggcgtt ttgctttgtt catcagacat tccctgtttc accgtttggc
    43081 aatgcacgtt cggcagctgc ttcagtacgg cgctttttca ttctttcatc aatcaggtat
    43141 tttcctgcca gcatcagtcc caggccaata aatgcacctg gtggcagcat cgccagcagg
    43201 aaaggggagt cggtgtggaa aatctccacg cgtaatactt ttgcccagct acctaacagc
    43261 gcatctgcac cgtcaaacaa tgtgccattg ccgataattt cgcgtagtga acccagcacg
    43321 aacatggcgc aggttgcgcc cataccaatt gaaaagccgt ccagtgccga aagcgccgga
    43381 ccttttttgg cagcgaaggc ttcagcgcgg cccacaacga tacagttagt gacaatcagc
    43441 ggaataaaaa tccctaatga ttgatacagg ccaaaggcgt aggcgttgat cagcatctgt
    43501 acagcgctga ccaccgaggc gatgatcatc acgtaaatgg gaatgcggat ctcggctggc
    43561 gtccagtgac gcagcgtcga aatggtcagg ttggtcagcg tcagtaccag cgtagtcgca
    43621 agtcctaaac ccagagcgtt agtggcagta gacgtgacag ccaacagagg acaaaggccg
    43681 agcaactgga ccagcgcaga gttgtttttc cacaacccct gaacaataac gtctttaatt
    43741 tcgctcacgc tttattctcc acaggcagga agttgagaaa gttgttccgg taacgtctga
    43801 gcgtacaatc cggcgcgttt taccgcatta accaccgcgc ggggagtaat cgtcgcgccg
    43861 gtgaactggt cgaaattacc accatctttc ttcaccgccc agtgcgcatc atccgcacca
    43921 ctgatttttt tacccgcaaa atgggtgatc cagtcagaaa ggcgcagttc gattttatcg
    43981 ccaagccctg gcgtttcgtg gtgctctgtc acgcgcgtgc caagtaccgt gccgttaaaa
    44041 tcggctccca ccagcagctg aatcgcaccg gaatagccat ctggcgcggt tgcttccaga
    44101 acggcggcta ccggtttgtc atcctgtttg gcgatgtaaa cccgatgctc acctttacct
    44161 aactctggcg cagttaccag atagcaactc tgtgccagcg cattgttata gcgttcggct
    44221 ggcagcacct gatcaaataa agccttttgt tgcagactgg cctgttcagc aatcgtcgtt
    44281 ttggtcatct ggttgatggc cgcagttaac cctgttgaac ccgctgcaaa tagcgccaac
    44341 gtaatgccgt gttttcggat agttttcagc atggtttacc ctttgcgatg gccgtagacg
    44401 cgcggacgcg tgtagtaatc gatcagagga accgtgatgt tcgccagcag gacggcaaaa
    44461 gccacgccgt caggatagcc gccgaaactg cggatcaacc agactaataa gcccgcaagc
    44521 gcgccgaaaa taagacgacc acgattggtc gtagaagcgg taaccgggtc agtcaaaata
    44581 aagaatgcgc cgagcatggt cgctccagac agcagatgaa tttgcggtgc tgccagtgtt
    44641 tctggtgaga acaaccagcc caacgttgcg cataacgcca gcgttactaa gaagctgagg
    44701 ggaatatgcc agcgaatcgc tttctgccat agcaaccata cgccgccagc cagccaggcg
    44761 agatttaccc attgccaacc agcgcccgcc agaataccgc tgtagatcgg atattgcata
    44821 atctgttcaa ccgaatgacc ggcacggaca gaggttttaa atgtatccag cggtgtcgcc
    44881 tgactaatgc catcaatacc taagcgtagt gtgttcatat caccaccact ggcggtatgc
    44941 ccgctgaaaa taacctgtat ggcgtcgata aaaccaggga tgttgaccgc aatttcatgt
    45001 ggcggtaacc agctggtcat ctgcaccggg aaggagatca gtaagaccac ataaccaatc
    45061 attgccggat taaacgggtt ttgccccaga ccgccataca actgtttagc gatgataacc
    45121 gcaaacaccg tacccagcac gaccatccac catggcgcga gggggggaat acttaccgcc
    45181 agcaataagc ctgtcagcaa tgctgagtta tctttcaacg tggcggctac cgactgcttg
    45241 cgtagtttga gtacgagagc ttcggctaac agagcactaa ccgatgccaa caggatctga
    45301 acgagagtac cccaaccaaa aaaccacagt tgcgctgcga ttcctggcac ggctgcgagc
    45361 aacaccaaca gcataatgcg cgatgtctgg cgctggttat gggtataagg ggagctagct
    45421 attctgaata ccatttagtc ctcgtttaca accttttgct ggacggcttt tttggcctga
    45481 acgcgggcaa tagccgcggc aactgccgct ttgcgcggat caacctgttc ttctggttct
    45541 gcattagcct gttgctgttc cagcttgcgt gctttggcgc gtgcaatagc cgctgcgacg
    45601 gcagctttgc gcggatcaac ctgttcttct ggctccgcat tagcctgttg ctgttccagt
    45661 ttgcgcgctt tggcacgggt aatagcggct tcgacggcgg ctttgcgcgg atcgaccggt
    45721 tcttctggtt ccgcattagc ctgttgctgt tccagcttgc gtgctttggc acgggcaata
    45781 gcggcttcga cggcggcttt gcgcggatcg accggttctt ctggttccgc attagcctgt
    45841 tgctgttcca gcttgcgcgc tttggcgcgg gcgatagcgg cttcgacggc ggctttgcgc
    45901 ggatcgacct gttgttctgg ttccgcatta gcctgttgct gttccagctt gcgtgctttg
    45961 gcacgggcaa tagcggcttc gacggcagct ttgcgcggat cgacctgttg ttctggttcc
    46021 gcatttgcct gttgctgttc cagcttgcgc gctttggcgc gggcgatagc tgcttcaacg
    46081 gcagttttac gtggatcagt aacggttgct gcgtcgttag tttgctgcag ttctgcctgt
    46141 ttcgctctgg cttgcgcttt acgggcttcc cgtgctgcaa taattgcact gttatccggg
    46201 cgttcgcccg ctttaatcac aataggctgt gtagcctggg cctgtttctc tttcacccgc
    46261 gccagagcgg cagcaatcgc atctttatct ttggctgcag gttgaacggc tgcgctctta
    46321 tgtcgttcaa ggcgagccgc tttttcgcgc tccagacgag cctggcgcgc ttcgaaacgc
    46381 gctttggctt ctgcggcgcg cttttcttcc tgacgaatag ccgcaatttc agctttttcc
    46441 tgacggaaat attgcaccag gggaatattg ctcgggcaaa cccacgcgca agccccacat
    46501 tcaatgcagt cggcaatgtt atgcgtggta gctttatcgt gttgctgacc tttgctgaac
    46561 cagtacaact gttgcggcaa aagatccgca gggcaggcgt cagcacaggc gctacaccgg
    46621 atgcagcttt gttcttcctg tggttcgcca agctcattgg cagagggagc caacagacag
    46681 ttggtaatct ttacgaccgg gacatccagc catggcaagg taaagcccat tagcgggccg
    46741 cccataatca ccatttgatc ggcagagggg cagaatccgg cgtcattcaa taaatgacgc
    46801 actggcgtcc ccagccgtgc ccagacgttg cccgggcgag cgattgcttc gccagtcagg
    46861 gttacgacac gctcggtaat cggctcgcca tcaataacgg cacgtttcac tgcataagca
    46921 gtgccgacgt tttgcattaa tacgccgata tcggatgaac gcccgccatg tggaacctgc
    46981 ttcccggtca gaatgtaggt taattgttta gcaccgccag aaggatattt ggttggaatc
    47041 acccgcatag aaatatcgtg agagtccgcc agcaccgcgc gcagcatgga aatcgcctgc
    47101 ggtttgttat cttcaatgcc gataagaatt tcgcgtggct gcagaatatg cgcaagaatg
    47161 cgaataccct ctacgacctg agccgcgcaa tcctgcatca aacggtcatc ggcggtaatg
    47221 tatggctcgc actcagcagc gttgataatc aacgtctcaa tcttatctcc gccgccctgc
    47281 aatttaacgc ctgtcgggaa tcctgcacca cccagcccgg caacaccaaa ctgatgaatg
    47341 cgctcgatta actcttcgcg acggcgagag cgataatcgg cccagccgtc tcgtgggatc
    47401 cagcagtctt caccatcggc atcaataatc acgcttaatt cagctaaagc tgaaggatga
    47461 gccgtagagt ggggcgcaat agccgtaacg gtacccgagg tgggcgcgtg aacaggcagc
    47521 attttgccgc gaccacgggt aagcggctgg ccgcgcaata ctttatcgcc gacgctaacg
    47581 cacaactcac cttcagcgcc aatatgctgt ttcagtggaa tgacaaaacg ctgcgccagt
    47641 ggtacctggc gcaggggtgt accgttggac tgggttttca tctccggtgg atggatgccg
    47701 ccgttgaaat cccagatttt attttttctg aatgcagaga ataacttaag catggtgttc
    47761 cacgggaatg atacgcacgg gaatggtgtt cagatcccat ttccaggagt caggtgtttc
    47821 tgcgaccggt tgcaacgaga tgcagtgcgt cgggcacgga tcaacacata aattgcagcc
    47881 cgtacagaga tcactcatta ccgtatgcat ggcacgggta gcgccaacga tggcgtctac
    47941 cggacacgcc tgaatacatt tagtgcagcc aatacagtta ttttcatcaa taaccgccac
    48001 catccgcgca ggcgttagct cttgcgcttc gccatccagc ggctgcggct cgacattaag
    48061 caactcggca atttttagca tcacagcttc gccaccaggg gcgcaacggt tgattttttc
    48121 accgttacag ctgatggctt ccgcgtaggg gcgacagccg ggataaccgc actgaccaca
    48181 ctggctctgc ggtaagattt cgtcaatttt ctcaacgacc ggatcgtctt ccaccgcaaa
    48241 acggcgggag gcataaccca gaatggcgcc aaacgccagg cccagcaggc tcacggcggc
    48301 aacggcaatc cagatagcat tcattacaac ttcaccaaac cactaaagcc cataaaggcc
    48361 agagacataa gacctgcggt aattaacgca atggcattac cgcgaaaagg tgccgggaca
    48421 tcagccacag caaggcgttc gcggatggct gcgaagagaa ccatcaccag cgaaaaaccg
    48481 acagcggcgg aaaaaccgta cagcgccgac tgcaagaaat tgtgcccgag attgatattc
    48541 agcaacgcca cgccgagcac ggcacagttg gtggtgataa gcggcaaaaa aatccccagc
    48601 aaacggtaaa gcaccgggct ggttttgcgc accaccatct cggtgaactg cacgaccaca
    48661 gcaatcacca gaataaatgc cagggtgcgc aggtaaatca gattaagtgg gatcaaaatc
    48721 cacgtatcga taagccaggc gcaaatagac gccagcgtca tcacaaacgt tgttgccagc
    48781 cccatgccca tcgcggtttc cagctttttg gaaaccccca taaacggaca gagaccgaga
    48841 aacttgacca gtacaaagtt attgaccagt acagttccga caaacagtaa caggtagtca
    48901 gtcatttttc ggcctgaaat aaaaaagccg cctattatcc gttaatccag agcaggcgac
    48961 aacaggttaa ctgtaaggtt attacggatt cacaaacgtc gctttcaccc gcgacgagcg
    49021 tttgaaatag gggacgatca atgcggttgc caacaaagta aacagcaact ggcgaacggc
    49081 tatgccatct tccacgggtg aaaaagcgaa cgcttttacc gccagcaata cggaaataag
    49141 cagccagata atatagtgtt taggaacaca acgccgacgt ttaaagaaag cgatggtcaa
    49201 ccacagtgtg taataccaca tggcgatagc ggtaataaaa gaaacgcccc ataagatttg
    49261 tgtcgtcagt gcttgtgcgc cgagcgtttg aaatgtttgc ggagaagata acgcagcggt
    49321 atacagcaac aacgccagcg tcgtacttaa cagagcgact aaaagccagg ccaacgggcc
    49381 aagcaaccag cctccaatac gttgtggcgt tgtggtggtc ataggttctc cagaatagcg
    49441 acgcaaacag gcaaatctaa ttttaggggc gttattataa ggcttttata accgatcgct
    49501 actttcttta ttgaacatag tgccagacgg acttcggtac ctggccgagg tcaaacaaac
    49561 gcccgccaga aaccagctct gcgcgacggt gatcggcagc acgatacata ttgatcagtt
    49621 cctgatcgtc cgtcagggtg taattaagat ggtcgtagag tttttccaga ctttcgagtg
    49681 aactgatttt gcgaaatttt aataagtagt cctgaacagt cataaaaggt ccataaaata
    49741 attaatacca aaggggtagg tattattagt gatgataaaa tttcaccaaa agtgaacgct
    49801 tgtgttatta caataataag aatgcgctga tggcaaatat tcagggatga aaaactgcta
    49861 tgaaaagagt aacgcgcttt actatccctg acaatcacca acaacaccga acaagataat
    49921 aaattcctgg tttaatatcc gacaagtgaa aacatgcacc ctgacgggca gcatgtcgct
    49981 ctacaagcgc agggcttact tgtgttgtac cgtagtactc tgttcaggtg gctgatagtt
    50041 gtcaatgtga ctcgccacgc caagaagaat gactgaaacg acaagaacga tccaacctgt
    50101 taattcaata agacgattca ttacaactca catctctgtt gattgatcca ttaacttcag
    50161 ggggtaaatg ttacttagca ataatagctc cagcaagatt tttactgagg tttttgcgat
    50221 atttagcttt tgtcgttgga aaaatcgcta tttttttgac ttaagttaaa caacatccct
    50281 tattgctggc aggttattaa actgttgagc gagggtaaag gatagtgtca aatagccatc
    50341 atacttcaat gagaggcaat gacatgagcg acaacatccg tgttgggttg attgggtatg
    50401 gttatgcgag caaaaccttc catgcgcccc tgattgcggg cacacccggg ctggaactgg
    50461 cggtaatctc cagcagtgat gaaacaaaag taaaagccga ctggccaacg gttacggttg
    50521 tctctgagcc gaagcatctg tttaacgatc ccaacataga cctgattgtc attcctacac
    50581 ccaacgatac ccatttcccg ttagccaaag cagcgcttga ggcgggtaaa catgtggtcg
    50641 ttgataaacc ctttaccgtg acactgtcac aagcgcgaga gctggatgcg ctggcaaaaa
    50701 gcctggggcg tgttctgtct gtattccata accgtcgttg ggatagcgat ttcttgacgc
    50761 taaaaggttt gctcgcggaa ggcgtgctgg gtgaagttgc ttactttgag tctcattttg
    50821 accgcttccg tccgcaggtg cgcgatcgtt ggcgtgaaca gggcggccca ggcagcggta
    50881 tctggtacga tttagcacca catcttcttg atcaggccat tatgctattt ggtttaccgg
    50941 tcagcatgac ggtagatttg gcacagttac ggcccggagc gcagtcgacc gattatttcc
    51001 acgccatctt gtcctatcca cagcggcgag tcattttaca cggtaccatg ctggcagccg
    51061 ctgagtcagc acggtatatc gtgcatggat cccgaggcag ttatgtgaaa tatggcctcg
    51121 atccacagga agaacgtctg aaaaatggcg agcgtctgcc gcaggaagac tggggctacg
    51181 atatgcgtga tggcgtactt acccgcgtgg aaggtgagga acgtgtcgaa gaaacgctgt
    51241 tgacggtgcc tgggaattat ccggcttact atgcggcgat tcgtgatgcg ttaaatggcg
    51301 atggtgaaaa tccggttccg gcaagccagg caatccaggt aatggagttg attgagctgg
    51361 gcatcgaatc cgccaaacat cgcgcgactt tgtgccttgc atgaaaaatg gatggtgcaa
    51421 tttgcaccat ccatttttgt tacttcgctg cgactttttc tcgcagtgcg cgtttttcct
    51481 ctgcgttgag gaaagccatt tccagaccat taatctgtgc ctggcggatt tgctcgcggg
    51541 ataacccagc agctggcgcg gcaacggtat attcgtgaat gatatccact ccctgtacgc
    51601 cgggatcgtc agtgttaatg ctggcacgaa tgccatgctc aaggaacgtt ttcagcggat
    51661 gtgcagccag ctctgccacg gtgctggtct gaatattgga ggtcagacag gattcaatac
    51721 caatttgttg ctcggcgaga aaatccatca gcgcccgatc ttcaatggct tttacgccat
    51781 gtccaatgcg ctctgccccc agttcacgaa tcgcctgcca gatgctttcc ggcccggcag
    51841 cttcgcctgc atggacggta atatgccagc ccgcatcacg cgcgcggttg aagtgagaaa
    51901 gaaacagact tcccgggaaa ccaagttcat caccggctaa atcaagtgcg gtaatctggt
    51961 cacggtgggc taaaaaggcc tccagctctt gctgacaggc ggcttcgccg aaggttcggc
    52021 tcataatgcc gataagcttc gcctgcacac caaaggtgcg gcaaccttca cgtacgccat
    52081 cgatcaccgc ttcaacaaca cctgcaacag gcagctgatg tgccattgcc atgtagcctg
    52141 gtgaaaaacg cagctcgaca tagtgcaggc cgttacgggc tgcatcttca atgttttcaa
    52201 atgccacgcg gcgacaagca tcaagagagg cgagaacttt aacgccccag tcaagtttgg
    52261 tcagaaagct caccagatcg ggttcgttgg caatgacctg aacgtgggga atcagtgttt
    52321 ccagggattg tgcaggaagc gagatattat actggcggcc aagttcaaga atggtctggg
    52381 gacgaatgtt gccatcaagg tggcgatgga tatcagttaa tggcagggtg gtatcaatca
    52441 tggtcgcact ctttttctgg atataaaagt gcgcaccatt ataaaaacaa agcagggtaa
    52501 aaatccacga ttgcgcaacg attttttacg ttgcgcaatt ggggttagcg aacagcgcgg
    52561 atggcgttaa ttaatccagc cacacctttt tccagtttcg aacgtgggca gccggcattg
    52621 agtcggacaa aaccacgacc ttcttcaccg taggtatacc ccggcatgat cgcgactttt
    52681 tcttgttcga taagtgcttt ttgcaacgcg ttgtcgtcaa tattcaacgg acgtaaatca
    52741 agccaggcca gataagtgga ttgcgggatc tgccagttga gttcaggaaa cgcggcgttc
    52801 attttatctg cgatatacgt caggttatct ttcagataga cgcgtaaggc atccagccac
    52861 ggtgcacctt gctgataggc ggcgatatgg gcagttaacg ccagtaccga aggggaagaa
    52921 agcccatcac gtcctttcag tgccgataaa taggcatcac ggctgctgct attttctata
    52981 atcccgtaag caccggtcag cgcgggaata ttgaaacttt tcgatcccga cgtcagcaac
    53041 gcccagtctc cccgcgccac attactccag ggaatatgcg gctgctcgcc ccaaaccata
    53101 tccatatgga tttcatcgga aataacccgc acaccatgac gctcgcacag gtcagccatg
    53161 atctccagct catcgcacgt ccacactttt ccggtaggat tctgtgggct acacaggagc
    53221 ataattttac attctggttt cgccaacacg gcttccagct tgcccatatc gcaaaaccag
    53281 ccgtcagcct gcttctctaa agcaacgggc attactgtgc gctgattacc ttcaatggcc
    53341 ttgtaaaatg cgtcataggc gggtgtgtgg atcaccacgc cttcacctgt ttcagaccac
    53401 tgacgaatca gttctgagac catatagata acagaagggc catacaccac cgactgagga
    53461 tcgatggcgg tgtaatgctg ggtggaaaac cagtgggcaa tagccgcgag aaactcatcg
    53521 ttcttccagc ggctgtagcc aaacacgccg tgcatcaggc gctgattcag cgcctcgata
    53581 atgcaggggg cagtggcaaa atccatgtct gaaatcgtga aaggtaacag gtcagcagtg
    53641 ccgaaacggt cagcgacata atcccactgc gtacaccatg tgccatgacg atccacgacc
    53701 tttgaaaaat cgaacatatc ttatccttat gcctggacag tatgcatcag accggccatt
    53761 tcatctttta ctgactgaac ttgtggcccg ataacaacct gcaggttatg ttgattaagt
    53821 tgtactacgc caattgcccg attgtccttc agtgcctgca cattaacaag cgacatatct
    53881 ttcacagaca aacgcagacg ggtaatgcag ttatcgaggc taacaatatt gtcggcaccg
    53941 cctaaggctt cgaggattgc cggaacgttg taacctgatt tacccggcgc accggcaacg
    54001 gctttttcga ttgaactggc cacttcgcta tcgcgccccg gggttttcag attgaagcgg
    54061 gtgatagcga aacggaagat gacgtagtaa acgacaaacc agattgccac caccactggc
    54121 accatgtacc acttggttga cagaccatgc aaaataccga acaccacgaa gtcgatgata
    54181 ttgccgtcgg tattaccgat ggtgacgccg agcacagaca tgacggtgaa gccgaggccg
    54241 gttaacagcg cgtggatgac atacagaact ggcgctacga acaggaacag gaattccagc
    54301 ggttcggtcg tgccgccaac gacacaggcg atcaggccag aaatcagcag ccctttaatt
    54361 ttatggcgat tttccgggcg cgcgcagtga tacatcgcta acgctgcacc tggcagaccg
    54421 ccgagaaacg caggcatttt gccttgcgaa aggaaacgcg tggcgctttc agaaaaaccg
    54481 tgagtggtcg ggcaactcaa ttgcgcctgg aagatggtca atgcgccgct gacggtttga
    54541 ccgcagactt cctgcgtgcc acctgcgtcg gtaaagcgaa ttaatgccac cagaatgtga
    54601 tgcagaccaa acggcaacag cagacgttca ccggtaccaa acagcatcgg tccgaaatca
    54661 cccgcgctat tgatcatatg gcctaagccg ctaataccca tggcgaaaat cggccagact
    54721 aatggaatca ccaggccgac aaggcccatc accagcgagg agatgattgg tacgaagcgc
    54781 gtaccgccga agaatgccag cgcatccggc aggcggatat tatggaatcg ctcatgcagc
    54841 atccagacga taataccggc gatcaccgca ccgaggatcc cggtgtcgat cgactggatc
    54901 ccaagaatgc tctggatgtt attggctttc agaacggcgg catccgtggt tggcagaatg
    54961 cctttattgg tcaaccagaa gtttaccgcg aggttcatta ccgcataacc gacgaagcca
    55021 gcgaatgccg ctacgccttt gttttctcgt gccagaccca gcgggatggc gatacagaac
    55081 atgactggca ggaaactaaa agcaaacgag ccaatcttac tcatccaggt aaagatagcc
    55141 tgcaacacgg ggttgcccag gaccgggagc agggtgatga catcgtggct actaagggaa
    55201 ctaccaatgc cgagcataat gccgcagaac gacaataatg ccacgggtaa catgaaggtt
    55261 ttgcctaact gctggaagaa ctcccacagc gtgactttcg gtgctgtttt cgccgtcata
    55321 aaacgactcc tcgtagagaa caatctggat tcagtaaatt gcgcgagaag ataaaacgtt
    55381 ttatcaaatt ttagtgaggc ataaatcaca ttacgcaacg ataatagcgg gtataagata
    55441 aataaaaggt aaaacgtttt atctgtcaca aaatcaggga gtaggtcatc tgcatggcta
    55501 ccgccaaaaa aataaccatt catgatgttg cgctggctgc gggcgtgtcg gtaagtaccg
    55561 tttcgctggt gcttagtggc aaagggcgaa tctctaccgc cacaggagaa cgcgttaacg
    55621 ccgccattga agagctggga tttgtgcgca atcgccaggc gtcggcgctg cgcggtgggc
    55681 aaagcggcgt cattggtttg atcgtccgtg atttatctgc gccgttttac gccgaattga
    55741 cggccggatt gacggaagct ctggaagcgc agggacggat ggtttttttg gttcacggcg
    55801 gtaaagacgg cgagcagctg gcacagcggt tttcactgtt actgaatcag ggtgtcgatg
    55861 gtgtggtaat tgccggggct gcaggaagca gcgatgacct gcgacggatg gcagaagaaa
    55921 aagctatccc ggtgattttc gcttcccgtg ccagttatct tgatgatgtt gatacggttc
    55981 gcccggacaa catgcaggct gcacagttgt tgacggagca tctcattcgc aatgggcatc
    56041 agcggatcgc ctggctggga gggcaaagtt cctcattaac ccgtgcagaa cgggtggggg
    56101 gctattgtgc aactctacta aaatttggcc tgccgtttca cagcgattgg gtgttggagt
    56161 gcacttccag ccagaagcaa gccgcggaag ctatcacggc gcttttacgt cataacccga
    56221 ccatcagtgc cgtggtttgc tataacgaaa ctattgcgat gggggcatgg tttggtttgc
    56281 tgaaagcagg gcggcaaagc ggggaaagcg gagtcgatcg ttactttgag caacaggttt
    56341 cgctggcggc atttaccgat gcgacaccaa ccacacttga tgatataccc gttacctggg
    56401 ccagcacgcc tgcgcgggaa cttggtacca cacttgcgga tcgcatgatg caaaaaatca
    56461 cccatgaaga gacgcattca cgcaatctta ttattcccgc ccggctcatt gcggcgaaat
    56521 aatcctctct ttatctgcta tacctggtag tgtcctttcc tcaagtttaa tgataaaaaa
    56581 tgtggcacac aggcggtgcc ttttattttt actgaagttc acaggaggtt tatgtgttta
    56641 attctgacaa cctgagactc gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta
    56701 ttggtaaaga aatcgccatt acattcgcga cagctggcgc atctgtggtg gtcagtgata
    56761 ttaacgccga cgcagctaac catgttgtag acgaaattca acaactgggt ggtcaggcat
    56821 ttgcctgccg ttgtgatatt acttccgaac aggaactctc tgcactggca gactttgcta
    56881 tcagtaagct gggtaaagtt gatattctgg ttaacaacgc cggtggcggt ggacctaaac
    56941 cgtttgatat gccaatggcg gattttcgcc gtgcttatga actgaatgtg ttttcttttt
    57001 tccatctgtc acaacttgtt gcgccagaaa tggaaaaaaa tggcggtggc gttattctga
    57061 ccatcacttc tatggcggca gaaaataaaa atataaacat gacttcctat gcatcatcta
    57121 aagctgcggc cagtcatctg gtcagaaata tggcatttga cctgggtgaa aaaaatattc
    57181 gggtgaatgg cattgcgccg ggggcaatat taaccgatgc cctgaaatcc gttattacac
    57241 cagaaattga acaaaaaatg ttacagcaca cgccgatcag acgtctgggc caaccgcaag
    57301 atattgctaa cgcagcgctg ttcctttgct cgcctgcagc cagctgggta agcggacaaa
    57361 ttctcaccgt ctccggtggt ggggtacagg agctcaatta atacactaac gaaccggtaa
    57421 acagccgtat gtgttgttta ccgggatagc gtgtgcaatt catcaacgtc tctgctaaac
    57481 aactggcagc caaatcacgg ccattggtta accaatagct ggatgaaaag catacgaata
    57541 gagtgtgcct tcgcactatt caacagcaat gataggcgct cacctgacaa cgcggtaaac
    57601 tagttattca cgctaactat aatggtttaa tgatggataa catgcagact gaagcacaac
    57661 cgacacggac ccggatcctc aatgctgcca gagagatttt ttcagaaaat ggatttcaca
    57721 gtgcctcgat gaaagccatc tgtaaatcct gcgccattag tcccgggacg ctctatcacc
    57781 atttcatctc caaagaagcc ttgattcagg ccattatctt acaggaccag gagagggcgc
    57841 tggcccgttt ccgggaaccg attgaaggga ttcattttgt tgactatatg gtcgagtcta
    57901 ttgtctctct cacccatgaa gcctttgggc aacgggcgct ggtggtcgaa attatggcgg
    57961 aagggatgcg taacccacag gtcgccgcca tgcttaaaaa taagcatatg acgatcactg
    58021 aatttgttgc tcagcggatg cgtgatgccc agcaaaaagg cgagataagc ccagacatca
    58081 acacggcaat gacttcacgt ttactgctgg atctgaccta cggtgtactg gccgatatcg
    58141 aagcggaaga cctggcgcgt gaagcgtcgt ttgctcaggg attacgcgcg atgattggcg
    58201 gtatcttaac cgcatcctga ttctctctct ctttccgcgg gctggtgaca actgagcccg
    58261 cgtttcatac tttaatttcc ctgtacaaaa tacaatctcg ccggagcggg gaattcctcc
    58321 ctaaaatatt caccgtagcc atatgtcatg agagtttatc gttcccaaca tgctcgaacg
    58381 aacgttcggt tgcttatttt atggcttctg tcaacgccgt tttaaagatt aatgcgatct
    58441 atatcacgct gtgggtattg cagtttttgg ttttttgatc gcggtgtcag ttctttttat
    58501 ttccatttct cttccatggg tttctcacag ataactgtgt gcaacacaga attggttaac
    58561 taatcagatt aaaggttgac cagtattttt atcttaatga ggagtccctt atgttacgtc
    58621 ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg
    58681 atcgcgaaaa ctgtggaatt gagcagcgtt ggtgggaaag cgcgttacaa gaaagccggg
    58741 caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt cgtaattatg
    58801 tgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca ggccagcgta
    58861 tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat aatcaggaag
    58921 tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg tatgttattg
    58981 ccgggaaaag tgtacgtatc acagtttgtg tgaacaacga actgaactgg cagactatcc
    59041 cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt
    59101 tctttaacta cgccgggatc catcgcagcg taatgctcta caccacgccg aacacctggg
    59161 tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg tctgttgact
    59221 ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg
    59281 ttgcaactgg acaaggggca ccagcgggac tttgcaagtg gtgaatccgc acctctggca
    59341 accgggtgaa ggttatctct atgaactgta cgtcacagcc aaaagccgga cagagtgtga
    59401 tatctacccg ctgcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat
    59461 caaccacaaa ccgttctact ttactggctt tggccgtcat gaagatgcgg atttgcgcgg
    59521 caaaggattc gataacgtgc tgatggtgca cgatcacgca ttaatggact ggattggggc
    59581 caactcctac cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga
    59641 acatggcatc gtggtgattg atgaaactgc agctgtcggc tttaacctct ctttaggcat
    59701 tggtttcgaa gcgggcaaca agccgaaaga actgtacagc gaagaggcag tcaacgggga
    59761 aactcagcag gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc
    59821 aagcgtggtg atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga
    59881 atatttcgcg ccactggcgg aagcaacgcg taaactcgat ccgacgcgtc cgatcacctg
    59941 cgtcaatgta atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct
    60001 gtgcctgaac cgttattacg gttggtatgt ccaaagcggc gatttggaaa cggcagagaa
    60061 ggtactggaa aaagaacttc tggcctggca ggagaaactg catcagccga ttatcatcac
    60121 cgaatacggc gtggatacgt tagccgggct gcactcaatg tacaccgaca tgtggagtga
    60181 agagtatcag tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt
    60241 cgtcggtgaa caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt
    60301 tggcggtaac aagaagggga tcttcacccg cgaccgcaaa ccgaagtcgg cggcttttct
    60361 gctgcaaaaa cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca
    60421 atgaatcaac aactctcctg gcgcaccatc gtcggctaca gccttggtga catcgccaat
    60481 aacttcgcct tcgcaatggg ggcgctcttc ctgttgagtt actacaccga cgtcgctggc
    60541 gtcggtgccg ctgcggcggg caccatgctg ttactggtgc gggtattcga tgccttcgcc
    60601 gacgtctttg ccggacgagt ggtggacagt gtgaataccc gctggggaaa attccgcccg
    60661 tttttactct tcggtactgc gccgttaatg atcttcagcg tgctggtatt ctgggtgccg
    60721 accgactgga gccatggtag caaagtggtg tatgcatatt tgacctacat gggcctcggg
    60781 ctttgctaca gcctggtgaa tattccttat ggttcacttg ctaccgcgat gacccaacaa
    60841 ccacaatccc gcgcccgtct gggcgcggct cgtgggattg ccgcttcatt gacctttgtc
    60901 tgcctggcat ttctgatagg gccgagcatt aagaactcca gcccggaaga gatggtgtcg
    60961 gtataccatt tctggacgat tgtgctggcg attgccggaa tggtgcttta cttcatctgc
    61021 ttcaaatcga cgcgtgagaa tgtggtacgt atcgtggcgc agccgtcatt gaagatcagt
    61081 ctgcaaaccc tgaaacggaa tcgcccgctg tttatgttgt gcatcggtgc gctgtgtgtg
    61141 ctgatttcga ccttcgcggt cagcgcctcg tcgttgttct acgtgcgcta tgtgttaaat
    61201 gataccgggc tgttcactgt gctggtactg gtgcaaaacc tggttggtac tgtggcatcg
    61261 gcaccgttgg tgccggggat ggtcgcgagg atcggtaaaa agaatacctt cctgattggc
    61321 gctttgctgg gaacctgcgg ttatctgctg ttcttctggg tttccgtctg gtcgctgccg
    61381 gtggcgttgg ttgcgttagc cattgcctca attggtcagg gcgttaccat gaccgtgatg
    61441 tgggcgctgg aagctgatac cgtagaatac ggtgaatacc tgaccggcgt gcgaattgaa
    61501 gggctcacct attcactatt ctcatttacc cgtaaatgcg gtcaggcaat cggtggttca
    61561 attcctgcct ttattttggg attaagcgga tatatcgcca atcaggtgca aacgccggaa
    61621 gtaattatgg gcatccgcac atcaattgcc ttagtacctt gcggatttat gctactggca
    61681 ttcgttatta tctggtttta tccgctcacg gataaaaaat tcaaagaaat cgtggttgaa
    61741 attgataatc gtaaaaaagt gcagcagcaa ttaatcagcg atatcactaa ttaatattca
    61801 ataaaaataa tcagaacatc aaaggtgcaa ctatgagaaa aatagtggcc atggccgtta
    61861 tttgcctgac ggctgcctct ggccttacct ctgcttacgc ggcgcaactg gctgacgatg
    61921 aagcgggact acgcatcaga ctgaaaaacg aattgcgcag ggctgataag cccagtgctg
    61981 gcgcgggaag agatatttac gcatgggtac agggaggatt gctcgatttc aatagtggtt
    62041 attattccaa tattattggc gttgaaggcg gagcgtatta tgtttataaa ttaggtgctc
    62101 gtgctgatat gagtacccgg tggtatcttg atggtgataa aagttttggc tttgccctcg
    62161 gggcagtaaa aataaaaccc agtgaaaata gcctgcttaa attaggtcgc ttcgggacgg
    62221 attatagtta tggtagctta ccttatcgta ttccgttaat ggctggcagt tcgcaacgta
    62281 cattaccgac agtttctgaa ggagcattag gttattgggc tttaacgcca aatattgatc
    62341 tgtggggaat gtggcgttca cgagtatttt tatggactga ttcaacaacc ggtattcgtg
    62401 atgaaggggt gtataacagc cagacgggaa aatacgataa acatcgcgct cgttcttttt
    62461 tagccgccag ttggcatgat gataccagtc gctattctct gggggcatcg gtacagaaag
    62521 atgtttccaa tcagatacaa agtattctcg agaaaagcat accgctcgac ccgaattata
    62581 cgttgaaagg ggagttgctc ggcttttacg cgcaactcga aggtttaagt cgtaatacca
    62641 gccagcccaa tgaaacggcg ttggttagtg gacaattgac ctggaatgcg ccgtggggaa
    62701 gcgtatttgg cagtggtggt tatttgcgcc atgcaatgaa tggtgccgtg gtggataccg
    62761 acattggcta tcccttttca ttaagtcttg atcgtaaccg tgaaggaatg cagtcctggc
    62821 aattgggcgt caactatcgt ttaactccca atttacactg acatttgcac cgattgtgac
    62881 tcgcggctat gaatccagta aacgagatgt gcggattgaa ggcgcgggta tcttaggtgg
    62941 tatgaactat cgggtcagcg aagggccgtt acaagggatg aatttctttc ttgctgccga
    63001 taaagggcgg gaaaagcgcg atggcagtac gctgggcgat cgcctgaatt actgggatgt
    63061 gaaaatgagt attcagtatg actttatgct gaagtaaaaa ataacgccgg agagaaaaat
    63121 ctccggcgtt ttggctgtga attactgctg cggaatagcg ggtacgaccg gaacatttaa
    63181 tgccggcatt gcaaacatac caacgaaatc ttccagtggc attttctgcc cgtttaacgt
    63241 tatctgaccg ttagtatatt gcaggctggt ggtgatggta ttgtcctgca aggtggtcag
    63301 acggaacatc tgccccattg ctgatgcacc ttcaacttgc tgtttcgcca gttttttcgc
    63361 ttgatcttcc tgataacctt ccagcttcgc tacctgagtc atcaactcag ttgccatatc
    63421 caccggaatg gtcagtttcg catccagaga tttaaccgaa cgatctactt cctgcgccag
    63481 cgtttgcggc gcttctttag tcgttgccgg atctttcagg aacagcgaca gattcagcgc
    63541 actttcaccc tgactgtttt tccagcttag cggcgcgata gtaatcaccg gatcgccttt
    63601 caacatcagc ggcagggcgc taaagaaggc ttccgtcact ttctcctgat aaagttcggg
    63661 attgttggca atttctggct gtgccagcag cgcctgagtt tgcgcgttat attgctggct
    63721 aaactgatgc caggcttcgc catcaatttg gccgactttt aaagtcagct tgccgctgcc
    63781 cagatcctga ttctgtacct tcaggctgtt tagcgagtaa tccagttggc tattgatcgt
    63841 tttaccgtca ttgaccagat ccgatttacc gctgatctcc atgccttcca gcagtgccag
    63901 ttctttgcct tccactgaaa tggtcatttt taccagtgac agtttttggt ttcctacgcg
    63961 ctcaccaaaa cttgccagcg tgctggaacc gtcggttttc agattattaa aggtcaactg
    64021 tactttctgg ttgtattcgt taaccgcgtc tatccgacca ctttgcgcct ccccggaaag
    64081 ggagatagct ttgccgtctc tgtccgcatt taactggaac tcgccgccgc taaaggcgac
    64141 tttttcatcc ttttgctcgt aatttagtgg cttgagcgaa atatcggaac tggaatcacc
    64201 gctgtaacca atgcgcgagt taatctcaaa aggcgtttca ccttttgcca tatcaaacag
    64261 tggtttgctt acttcgttat taaccagcgt ggtttgaatt gatgccatcg acgggatcag
    64321 gttcagtttt ttaagctggg caagggggaa gggaccatga tcaaccgatt cgttgaagat
    64381 gacgctctga ccgcttttaa tccacggatt ttcttttccg gcaatgggtt tcaccaacag
    64441 ttgcagctga ctgctgaata cgccgcgatg atagttttga taactcactt ccaggttgga
    64501 ttcaggagcg gtcagtttga gttgcgcgtt cgcctgcgcg accatgtctt cgagatgggt
    64561 ttcaatcttc ttgcctgtat accatgcgcc gcctgtccag actacgccta gcgcaacaat
    64621 gacgcctacc gctaccagcg atttattcat aatgattatc cataaaatga aatcaggcgg
    64681 actggccgcc tgaaggtgtt ataagccttt aataagctta gcaagagatg ttaatttttt
    64741 cagtaagctc ttacagcttg ttgtaaaccc gtgctaaacg gccgtggcct ttgacagtca
    64801 ccggtgattc gttggcggca ataaacgctg attcacccgg tttaagctgt aactgctgag
    64861 aacctttcca caacgttgca tcgccttcga cgcagaacaa aatggcggca ctctgctggc
    64921 taatggtggt ttctttatca ctaaggtcat gcagcgagaa ggcaaaatca tcaacaggaa
    64981 tcgggaagtc cagttctgca ccttgtttca ccggctgggt caacaactgg ttagccggtt
    65041 tggcttcgaa cttcacattg gcaaccagtt ccggaatatc aatgtattta ggcgtcagac
    65101 ccgcacgcag cacgttatcg gagtttgcca tcacttccag cgccacgcct tgcaggtaag
    65161 cgtgcggtgt ttcagcgaac aggaacatcg cttcgccagg atttaatttc accacattca
    65221 gcaacagcgg agagaacagg ccgctgtcat ccgggtaaaa ttcagaaatt aaacgaatcg
    65281 tttgccacgg ttcaccctgc tgactgtcga gcgccgattt taaaatcgcc agcgcgcggg
    65341 atttttcttc accctgcata ttcaacaggc tggcgaacag ttcgcttaaa cgttcggcat
    65401 caggctgttg taaaaagtga gcaatcgccg gatgtgcacc tgcgaccggc tggagtaggg
    65461 agacaatctc ggaaaattca cgaaacgcgt tcatcgcaag gaaaggcgtc agcgcaaaaa
    65521 ccagctccgg cttgtggtta ggatctttat agttacgctc ggcggcatcc atcgggatac
    65581 ctgcggcatt ttctttggca aaaccgattt cagacttgtg tttgtttgga tgaacctgaa
    65641 tggagagtgg ctgtgctgcg cataatactt tgaacaggaa aggcagttcg ccaaagcgtt
    65701 tggcaacggc ctctccgagc agagtcgatt tatcactctc aatcacatca cgcagtgaaa
    65761 cgatatctcc ggcggcattc tgcactcgtg aactgctttt cggatgtgcg cccatccaca
    65821 gctcggccat cggctggctg gacggatttt ccataccata aagttcagtc aacgccgttt
    65881 tgctgcccca ggcatagttt tgcactgagt taatgagttt ttgcatgatc aatccctgtt
    65941 ttaatgtgga aattaatccc gctattaaag caagaatcct acgggaagta acctggagcc
    66001 gcaaaaagtc gtactagtct cagtttttgt taaaaaagtg cgtaggatat tgttactcgc
    66061 ttttaacagg gcaacggaac acccgcccag agcataacca aaccaggcag taagtgagaa
    66121 aacaatgtca aacaaaccct ttcattatca ggctcctttt ccactcaaaa aagatgatac
    66181 tgagtattac ctgctaacca gcgaacacgt tagcttatct gaatttgaag ggcaggagat
    66241 tttgaaagtc gcacccgaag cgttaactct gttggcgcgc caggcgtttc atgatgcgtc
    66301 gttcatgctg cgtccggcgc accaacaaca ggtggccgac attctgcgtg acccggaggc
    66361 cagcgaaaat gataaatatg tggcgctgca attcctgcgt aactccgaca tcgcggcgaa
    66421 aggcgttctg ccaacctgtc aggataccgg caccgcgatt attgttggta aaaaagggca
    66481 gcgtgtatgg accggtggtg gtgatgaagc ggcgctggcg cgcggtgtct ataacactta
    66541 tatcgaagat aatctgcgct actcgcaaaa cgcgccgctg gatatgtata aagaagtgaa
    66601 taccggcacc aatctgccag cgcagatcga tctttatgcc gttgatggcg acgaatacaa
    66661 attcctctgt atcgccaaag gtggtggttc ggcaaacaag acgtatctct atcaggaaac
    66721 caaagcgtta ctgacgccgg ggaaactgaa aaattacctg gttgagaaga tgcgcacgct
    66781 gggtacggcg gcctgtcctc cgtatcatat tgcgttcgtt attggtggaa cttctgcaga
    66841 aacgaacctt aaaacggtga aactggcttc cgcgaaatac tatgatgaac tgccaacgga
    66901 agggaatgag cacggtcagg cgttccgcga tgtggaattg gaaaaagaat tgctgatcga
    66961 agcgcaaaat cttggtctgg gtgcgcagtt tggcggtaaa tacttcgctc acgacatccg
    67021 cgtgattcgc ctgccacgtc acggcgcatc ctgcccggtc ggtatgggcg tctcctgttc
    67081 tgctgaccgt aatatcaaag cgaagatcaa ccgtcagggg atctggatcg aaaaactgga
    67141 acataatcca ggcaaatata tcccggaaga gctgcgcaaa gcgggagaag gcgaagcggt
    67201 gcgcgttgac cttaaccgtc cgatgaaaga gatcctcgca cagttgtcgc agtatcccgt
    67261 ttctacacgc ttatcgctta acggcacgat tatcgtcggt cgtgatattg ctcacgccaa
    67321 actgaaagag cggatggata acggtgaagg gctgccgcag tacatcaaag atcatccgat
    67381 ttactacgcg ggtccggcca aaacgccgga aggttatgcc tccggttctc ttggcccaac
    67441 gaccgccgga cggatggatt cttatgtcga tcaactgcaa gcgctgggcg gaagtatgat
    67501 catgctggcg aaaggcaacc gcagccagca ggtgacggat gcctgtaaaa aacacggtgg
    67561 cttctacctt ggcagtatcg gtggtccggc cgctgtattg gcgcagggaa gtattaagag
    67621 cctggaatgt gttgaatatc cggaactggg aatggaagcc atctggaaaa ttgaagtgga
    67681 agatttcccg gcgtttatcc ttgtggatga taaaggaaat gacttcttcc agcagataca
    67741 actcacacaa tgcacccgct gtgtgaaata aacagagccg cccttcgggg cggttttttt
    67801 acatggcacg aaagaccaaa catttgttat caaatagtaa ataatgagtg agctaaaagt
    67861 tacttaacga aagcaaaaca gaaagaaaaa attaatcagg tgaggagtag gccatgaata
    67921 cagtacgcag cgaaaaagat tcgatggggg cgattgatgt cccggcagat aagctgtggg
    67981 gcgcacaaac tcaacgctcg ctggagcatt tccgcatttc gacggagaaa atgcccacct
    68041 cactgattca tgcgctggcg ctaaccaagc gcgcagcggc aaaagttaat gaagatttag
    68101 gcttgttgtc tgaagagaaa gcgagcgcca ttcggcaggc ggcggatgaa gtactggcag
    68161 gacagcatga cgacgaattc ccgctggcta tctggcagac cggctccggc acgcaaagta
    68221 acatgaacat gaatgaagtg ctggctaacc gggccagtga attactcggc ggcgtgcgcg
    68281 ggatggaacg taaagttcac cctaacgacg acgtgaacaa aagccaaagt tccaacgatg
    68341 tctttccgac ggcgatgcac gttgcggcgc tgctggcgct gcgcaagcaa ctcattccgc
    68401 agcttaaaac cctgacacag acgctgagtg aaaaatcgcg tgcatttgcc gatatcgtaa
    68461 aaatcggtcg aacccacttg caggacgcca ccccgctaac actggggcag gagatttccg
    68521 gctgggtagc gatgctcgag cataatctca aacatatcga atacagcctg cctcacgtag
    68581 cggaactggc tcttggcggt acagcggtgg gtactggact aaatacccat ccggagtatg
    68641 cgcgtcgcgt agcagatgaa ctggcagtca ttacctgtgc accgtttgtt accgcgccga
    68701 acaaatttga agcgctggcg acctgtgatg ccctggttca ggcgcacggc gcgttgaaag
    68761 ggttggctgc gtcactgatg aaaatcgcca atgatgtccg ctggctggcc tctggcccgc
    68821 gctgcggaat tggtgaaatc tcaatcccgg aaaatgagcc gggcagctca atcatgccgg
    68881 ggaaagtgaa cccaacacag tgtgaggcat taaccatgct ctgctgtcag gtgatgggga
    68941 acgacgtggc gatcaacatg gggggcgctt ccggtaactt tgaactgaac gtcttccgtc
    69001 caatggtgat ccacaatttc ctgcaatcgg tgcgcttgct ggcagatggc atggaaagtt
    69061 ttaacaaaca ctgcgcagtg ggtattgaac cgaatcgtga gcgaatcaat caattactca
    69121 atgaatcgct gatgctggtg actgcgctta acacccacat tggttatgac aaagccgcgg
    69181 agatcgccaa aaaagcgcat aaagaagggc tgaccttaaa agctgcggcc cttgcgctgg
    69241 ggtatcttag cgaagccgag tttgacagct gggtacggcc agaacagatg gtcggcagta
    69301 tgaaagccgg gcgttaatct gcaacataca ggtgcagccg tggaatgatc aaacgaagcg
    69361 gctgcgcctg aggtttataa cggtgctgca cattgtcggc atcgtaattt aacaactcac
    69421 caacgtccgg cacgcccgca ccattatccc gattaatcag tgcaatcagc ggtgtagggc
    69481 aggcgtgttg gacttgtttt tgatctcctt tgtaccagac gcgggcaatc ggctgcacct
    69541 tcaccggacg tttgattttt aacttcgcat tctgtggcag ggcagcgata tcctgatact
    69601 ctcgctccag ttttctttgc cactcctcgc gcgtccacgg tgcgacactg cgtggtgatt
    69661 tcaggctttt ttccagctgt gccaggactt catcacgatg caaattctta atgatatgtt
    69721 tattagccca accaaagcgt aaagtggcgg gatcgtgcag aacggtgagc gtgcggtaag
    69781 cattaagggt gatcagcccc ggcaaatgac gatgcaccca ttcaaaacgt gccgcggtgg
    69841 ggagttctga ttcaaccgtg acgatatgct cgaacgtggt cttgagttta ttgatgtgct
    69901 gaatatgact gaccaacgct gcttgcgaaa ggttatcgac ctggtaacac aacacgccag
    69961 gcagacggac agctgctttg ctgctgcgat tttcggactg ttgttgaata aataaatgac
    70021 ggaaatgacg caacgcctgc gactgcgcgt cgttgccgag atgttgtttc acctcaatac
    70081 gattaagcgg attatgctca tcctcttttt ttacctccgg caaagagaac acgcgggcaa
    70141 ccaatagctt gtgttgctca agatgagcgg caaatgcagc cagctcttgt tccatctggc
    70201 gaaaagtagt gttgagtcgg tctacgagat cgtaacgcgc cataatatta accactttag
    70261 ttacaacatc cttaatttat agcacagtgg tggtgacttg accaccgtgc gcgtagtgtc
    70321 aagcagaggt aaattgcggg atgttatgcc ataacggcca gctaaacgag aagcgggcac
    70381 cacccagttc gctggtgtca caattaaccg taccgcccat tgccagtgct atagagtgga
    70441 caattgccag ccccagcccg cagccgccgg ttgagcgatc ccggctggga tcgaggcgaa
    70501 caaaaggttc aaagatatgt tcgcggtttt ctggggcaat ccctgggcca tcatcctcaa
    70561 caattaatgt cgctctattc cccgacagta gcaggctggt ttcaaccgtt gaatggcagt
    70621 agcgcagggc attattgagc aaattatcca gcacgcgctc cattaagcgc atatccaacg
    70681 ccgcataatg gccttgcatg agcgttttta tccgtaccgt tttatcgggc gttactgcct
    70741 gaatatctgc cagatgcgtt gacagccaca acggcaggtc tggttcgcta agatgaagct
    70801 cgttttgtgg gcgatcgagt cgggcataag tcagcagctc ttcaattaaa gcttcaagtt
    70861 gactgatatc acgattcaac gcctgggatt cggcggcgct caggttatcg ctcatctcca
    70921 gtcgataacg caggcgcact aacggtgttc gcagttcgtg agcgataccg tcaataagct
    70981 gttttttgct ggcaatcaag gcgttgatat tgtccgccat ctggttaaat gcgataccaa
    71041 gtcgttcaaa gctcgaaccc tcatcaaagt ggatacgttc attgagatgc ccatcgccaa
    71101 atcgttgcgc cgctgcttcc agttttaaca tatcctgcca gtgcggacgc atccagataa
    71161 acaccggaaa ggcgagggaa atagcaataa aagcgatcag ggcgatatcc agcaatcgca
    71221 tctgatggag ataataaaga taaggaacag gaccaactgc cagcacgtag tggctgcgcg
    71281 ggatacgctg caaaaaagtg tactgatcgt ccagggcgac aatttcgccg ccacgcagcc
    71341 ggtgcatgga aatatcatca agatggtatt tactcagtgg ctcgactcgc agatcgaaag
    71401 agagatttaa atccatctct ttcagcgtct taccccagtc atgtgggggg atctcacgca
    71461 attcgctgcg catcagatac agcgaactgt tcatcaaatc atccagcgac tgtttgcccg
    71521 cgcgttcggc ggtaaatttg tacaccaacc caaccagcag agacatcaca aggaagcaga
    71581 caaacaataa caggtaaaac tggataaaca gttttttcat cgcttattcc catgcatgag
    71641 gcgcaaaaag atagcctttg ttacgcacag ttttaatgcg ataaggttca gcggcgttat
    71701 cgagcagttt ttttcttaac cgcgaaatag ccacgtccac gctacgatcc aggccgtcat
    71761 aactgacgcc gcgtaaattt ttcagcaatg catcgcggtc catgatttgc ccggcatggg
    71821 tagctaattc ccacaataat tcgaaatcag ctgtcgagag cgagatttca gtgttagcca
    71881 gggtgactac gcggttgata ggatcgatgg tcaacgtgcc gaaatgcagg gctttgtagg
    71941 gagtcagtga cgtttcctga agacctttgg tcagcgtggc ttgctcattc tgacgcaaat
    72001 gcaaacgtaa acgcgctaac aaaacagcag ggggcgtcgt tttgagaata tagtcgcagg
    72061 cacccatttc cagtgccagg atgtggttca tatcgctatc gagagaggtt agaagaacaa
    72121 tcggtccgga ccactttgcg cgtaaatcac gacaaatggt catgccgtcc ttgcctggta
    72181 gcatgatgtc gagtaacacc aaatccggat tttctcgcaa aatggtttct tcggcctggt
    72241 cgccgcgcgg ctctacggta acctgtatat catgttttgc caggtacgcg gcaatcagtg
    72301 aaccgacttc cgcatcatct tccacaaata cgatagtgtt cataacattc acggtagata
    72361 taaaaacgtc acaatacacc gcgctgtttt tacttactat caatcgtttc taaacaagtt
    72421 ttcatcaggt attctactga tgacttgtta tggaagtgtt aaggtaaaaa gatggggctg
    72481 gtaatcaaag ccgcgctcgg tgcgctggta gtgctgttga ttggtgtttt agcaaaaacg
    72541 aaaaattatt atatcgccgg gctgattcca cttttcccga cctttgcgct tatcgcgcat
    72601 tatattgttg ccagcgaacg cggcattgaa gccttacgcg caaccatcat ttttagtatg
    72661 tggtcgatta ttccctattt tgtttacctg gtgtcgctgt ggtatttcac cggaatgatg
    72721 cgcttgcccg ccgcttttgt tggttctgtt gcgtgttggg ggataagcgc atgggtgttg
    72781 attatatgct ggataaagct gcattaacgc agatgacgac cgccatcaag tgggaaactg
    72841 cgtccggtga caaagcaact ggtaagtaag taatcgacca ggtcgatcac ttctttctcg
    72901 ccaggcgcgg ttttcatcaa tgatttattc agcgcctgtt gtcgatattc ggcatcatca
    72961 tgttcattaa acaggatcag cgatggcgca atagaattca ctttcacttc cggtgccagc
    73021 ttgcgggcaa acgagcgggt catattatcc agtgccgctt tgcttgcagc atacgcaata
    73081 tgtttgtcgc taccgcgctc caccacataa tcggtaaagt gaatgatatc gctggcggcg
    73141 tgtccgtgcc cacgcagtaa tctttccagc gcatggttga gcaggtatgg ggtattaacg
    73201 tggatctgca tcatgcaagc cagtacgtcg gccagtggcg cacccggttt ttccgccatc
    73261 cacgcactgg cgttatgcaa aatagcacgc agaccatggg tgctttttag tacttcatcg
    73321 gcaaacgcca tcacaccgtc gttggtcgaa aaatcagcct gaatacactg cgcacctgca
    73381 ttaatcagtc catcaatggc tggatagtgt gtccgatagc tgacaatcac cggttgcttt
    73441 tgattaatga aatgccatgc gagggcgagg ccgatgcgac gacctccgcc agtaattaat
    73501 attggcaagg gctgggtttt acccatcgtt atctcctttg ctatccaacg atgggatgaa
    73561 cgttatccca ccagcatcca ggtcgccgga actgaggcaa ttaacagcat gccaatcagc
    73621 accatttcct gacggttcag tacattatca tgtgtatgcg ttttgcgcgc atacaggaaa
    73681 accagcagcc ccggtgcata cagaacaacg gataacagca agtgcattgg accagaggcg
    73741 tacaataacc ataagccata aatgcaggca ccgacaccta ccgctttatg cagtggacgg
    73801 gttgcgattt tcagcaagaa cgcgccgacc aggaaatagg gcaccagaat catttctgag
    73861 gcgatggtca gcaacgtgtt gtaatcggaa ccggtcagcc agatgagtac cagacaaatt
    73921 tgcacgcaga tgtttgtcaa ccacagtgag gcggatggcg cagcttgtgc gttctggcgc
    73981 gcaaaaatgc gtgggaatgc tttatgagtg gcagccagga acggaacttc tgccgccatg
    74041 atggtccagc ttaagtacgc gccgcaaacg gaaacgatca aaccggcagc gatgatgatt
    74101 tcgccccacg gtcccatcat ttccaccatc agaccggcca ttgacgggtt acgaatttca
    74161 gccagttcag gacgtgccac cactcccagc gaaagcagcg ttaccagcaa gtaaacgccc
    74221 agagcggaga gaaccgccag cagtgtcgct ttgccaacat cacgtttatt acgcgcacgc
    74281 gcagaaacaa ccacggcacc ttccacacca atgaaaaccc acagggtgat cagcatggtg
    74341 tttttcacct gttcccaaac aggtacgcca agtgcaagtc cggtgaagtc gagcttgaag
    74401 atatccagtt tgaacatcat catcgccagc acaacaaaca gacccagcgg caacaatttt
    74461 gccagtgtcg ccaccaggtt aatgctggca gcggtttgca ccccgcgcag gatcaaaaag
    74521 tgaacaatcc acaataaagc cgatgcaccg actatcgatt gccaggtatt accatcgcca
    74581 aacaggcgca attccggcgt gtccgtaaaa aagcttaacg cggaaaaaac gatcaccaga
    74641 taggagacgt tggcgatgac tgcgcacagc cagtatcccc atgcggaaca aaagccgatt
    74701 agctcgccaa acccttcgcg ggcataggta aagataccgc cgtcaagttc gggacgaatg
    74761 cgcgtgagga tcagcatggc aaaggccagc aataaaatgc cagcgccagt aataccccag
    74821 ccgatgagca gtgctgccgg gctggcaact gccgccatat tttgcggcag actgaaaaca
    74881 cccgcgccca gcattgagct taataccagc gcggtgagtg cgctcagtcc cagtttcttt
    74941 tccatcgata tccagtgatt aaaacgtgat aagaagaata aatattaggg caaaacgcat
    75001 aaaaatggag tttgccactg aagcgcggga ttttacgaag agatgtgtct gcatgcaatg
    75061 gtcgcgggga aatttgtgac taaaaatgcg aaaggcggca tcaagccgcc ttataagagt
    75121 aactgaatag ttatttatac agatctgcac tgacggtcag gttattacca cgttcctgcc
    75181 actggcgggt gatgtggtaa tatttagcac ctttcttcgc ggctcgtttc gcaacctgat
    75241 aggagacttc ggtcatgttg ccgtagttgc cagagaattt gatgctgtcg aacgggacca
    75301 tcatcgctgc tgtcgctttg ttcagttctt cgactttagt gccatccgga agcgtgacag
    75361 tgtaacgccc gccttttgat gactgggttt caaagaagcg accgacttca gaacttggtg
    75421 atgcggtagt cgcaacaccc gggatctcaa ctttcttcgc ggcttcgcca ccggcagcca
    75481 gagctgcacg tcctgcttcg gaatctgccg ggatcacatc cgggctctgg acgatacgtt
    75541 tcttagcatc ttttttatag atgaatgcag taatacgctg gttgccgccc tggttggcat
    75601 cgatttgacg aacgatgtag aaagagtagg cacctttcgc ttttgccgct ttggtgatgg
    75661 catcattgac ttctggctgg ctacgataga agccctggac ggtgaccgtg tcaaacggtt
    75721 caatcagaac agcctgatct ttcggcagtt cgacaacacc gttaattacg cgattacttg
    75781 tttcttctgc tttttcagca tcggctttat agaggtcagc gaccacacgc cagttaccgc
    75841 tgttcccaaa atcagaagtg tcgacaacat aaaaagaggc ggcaccttct ttatctgcgc
    75901 gacgagaaac ggctttcacc gcttcgccaa tagcattaaa acgaccggta accactacac
    75961 ggtcaaaagg tttaaccgct gccgcttgct ccggtgtcag ttctgttgct gcgttaacgg
    76021 agaatgccgt agcagaaagc agtgccgacg ccaggagggt gttcttaagc ttcataaaaa
    76081 taatccttcg ccttgcgcaa accaggtact ggtattgtta ttaacgagaa acgtggctga
    76141 ttattgcatt taaacggtgt aactgtctgc gtcatttttc atatcacatt ccttaagcca
    76201 attttaatcc tgctcaaatg accgtctatg cttaaaaaac agccgtatca gcatcattac
    76261 tactgaagca actgaattgt ataagttaat ttaatgttaa gtagtgattc gtgccggggc
    76321 gatgtctcgt tttacccgac cgtcgaagac aattatcagt ctttatccgg cgttctaagg
    76381 tgtttatccc actatcacgg ctgaatcgtt aatattttgc gagttcacgc cgaaatactg
    76441 atttttggcg ctagatcaca ggcataattt tcagtacgtt atagggcgtt tgttactaat
    76501 ttattttaac ggagtaacat ttagctcgta catgagcggc ttgtgtggct cctgacacag
    76561 gcaaaccatc atcaataaaa ccgatggaag ggaatatcat gcgaattggc ataccaagag
    76621 aacggttaac caatgaaccc cgtgttgcag caacgccaaa aacagtggaa cagctgctga
    76681 aactggggtt taccgtcgcg gtagagagcg gcgcgggtca actggcaagt tttgacgata
    76741 aagcgtttgt gcaagcgggc gctgaaattg tagaagggaa tagcgtctgg cagtcagaga
    76801 tcattctgaa ggtcaatgcg ccgttagatg atgaaattgc gttactgaat cctgggacaa
    76861 cgctggtgag ttttatctgg cctgcgcaga atccggaatt aatgcaaaaa cttgcggaac
    76921 gtaacgtgac cgtgatggcg atggactctg tgccgcgtat ctcacgcgca caatcgctgg
    76981 acgcactaag ctcgatggcg aatatcgccg gttatcgcgc cattgttgaa gcggcacatg
    77041 aatttgggtg cttctttacc gggcaaatta ctgcggccgg gaaagtgcca ccggcaaaag
    77101 tgatggtgat tggtgcgggt gttgcaggtc tggccgccat tggcgcagca aacagtctcg
    77161 gcgcgattgt gcgtgcattc gacacccgcc cggaagtgaa agaacaagtt caaagtatgg
    77221 gcgcggaatt cctcgagctg gattttaaag aggaagcggg cagcggcgat ggctatgcca
    77281 aagtgatgtc ggacgcgttc atcaaagcgg aaatggaact ctttgccgcc caggcaaaag
    77341 aggtcgatat cattgtcacc accgcgctta ttccaggcaa accagcgccg aagctaatta
    77401 cccgtgaaat ggttgactcc atgaaggcgg gcagtgtgat tgtcgacctg gcagcccaaa
    77461 acggcggcaa ctgtgaatac accgtgccgg gtgaaatctt cactacggaa aatggtgtca
    77521 aagtgattgg ctataccgat cttccgggcc gtctgccgac gcaatcctca cagctttacg
    77581 gcactaacct cgttaatctg ctgaaactgt tgtgcaaaga gaaaaacggt aacatcactg
    77641 ttgattttga tgatgtggtg attcgcggcg tgactgtgat ccgtgcgggc gaaattacct
    77701 ggccggcacc gccgattcag gtatcagctc agccgcaggt ggcacaaaaa gcggcaccgg
    77761 aagtgaaaac tgaggaaaaa tgtgcctgtt caccgtggcg taaatacgcg ttgatggcgc
    77821 tggcaatcat ccttttcggc tggctggcaa gcgttgcgcc gaaagagttt cttgggcact
    77881 tcaccgtttt cgcgctggcc tgcgttgtcg gttattacgt ggtgtggaac gtatcgcacg
    77941 cgctgcatac accgttgatg tcggtcacca acgcgatttc agggattatt gttgtcggag
    78001 cactgttgca gattggccag ggcggctggg ttagcttcct tagttttatc gcggtgctta
    78061 tagccagcat taatattttc ggtggcttca ccgtgactca gcgcatgctg aaaatgttcc
    78121 gcaaaaatta aggggtaaca tatgtctgga ggattagtta cagctgcata cattgttgcc
    78181 gcgatcctgt ttatcttcag tctggccggg ctttcgaaac atgaaacgtc tcgccagggt
    78241 aacaacttcg gtatcgccgg gatggcgatt gcgctaatcg ccaccatttt tggaccggat
    78301 acgggtaacg ttggctggat cttgctggcg atggtcattg gtggggcaat tggtatccgt
    78361 ctggcgaaga aagttgaaat gaccgaaatg ccagaactgg tggcgatcct gcatagcttc
    78421 gtgggtctgg cggcagtgct ggttggcttt aacagctatc tgcatcatga cgcgggaatg
    78481 gcaccgattc tggtcaatat tcacctgacg gaagtgtttc tcggtatctt catcggggcg
    78541 gtaacgttca cgggttcggt ggtggcgttc ggcaaactgt gtggcaaaat ttcgtctaaa
    78601 ccgttgatgc tgccaaaccg tcacaaaatg aacctggcag ctctggtcgt ttccttcctg
    78661 ctgctgattg tatttgttcg cacggatagc gtcggcctgc aagtgctggc attgctaata
    78721 atgaccgcca ttgcgctggt atttggctgg catttagtcg cctccatcgg tggtgcagat
    78781 atgccggtgg tggtgtcgat gctgaactcg tactccggct gggcggctgc ggctgcgggc
    78841 tttatgctca gcaacgacct gctgattgtg accggtgcgc tggtcggttc ctcgggggct
    78901 atcctttctt acattatgtg taaggcgatg aaccgttcct ttatcagcgt tattgcgggt
    78961 ggtttcggca ccgacggctc ttctactggc gatgatcagg aagtgggtga gcaccgcgaa
    79021 atcaccgcag aagagacagc ggaactgttg aaaaactccc attcagtgat cattactccg
    79081 gggtacggca tggcagtcgc gcaggcgcaa tatcctgtcg ctgaaattac cgagaaactg
    79141 cgcgctcgtg gtatcaacgt gcgtttcggt atccacccgg ttgcggggcg tttgcctgga
    79201 catatgaacg tattgctggc tgaagcaaaa gtaccgtatg acatcgtgct ggaaatggac
    79261 gagatcaacg atgactttgc tgataccgat accgtactgg tgattggtgc taacgatacg
    79321 gttaacccgg cggcgcagga tgatccgaag agtccgattg ctggtatgcc tgtgctggaa
    79381 gtgtggaaag cgcagaacgt gattgtcttt aaacgttcga tgaacactgg ctatgctggt
    79441 gtgcaaaacc cgctgttctt caaggaaaac acccacatgc tgtttggtga cgccaaagcc
    79501 agcgtggatg caatcctgaa agctctgtaa ccctgacggc ctctgcggag gccgtcactc
    79561 tttattgaga tcgcttaaca gaacggcgat gctttgacct cccgctgttt gttcaagcgc
    79621 aattttgaca ataattgtta acggcacgga aagcagcata cccaccggtc ctaacaacca
    79681 tccccaaaaa atcaacgaca aaaataccac caatgtggaa agtcccagcc cacgccccat
    79741 gatgcgtggc tccagaatat tgccgaagac cagattaatc agcagatatc ccgccagcac
    79801 caacaacgct tcgtagaagc cattaaacac cagtacctga gcaatagggg ggattgccgc
    79861 gagaactgaa ccaatattag ggatgtaatt aagcgcaaag gccagcaatc cccagacaaa
    79921 agcgaagcga acatcgaggg cggcgagcat cgcccatgcg accaggccgg tgatgatgct
    79981 gatggctgtt ttcagcacca gataatgaga cacactgtca atcgcacgct gaatcgccgc
    80041 catcccttca accggacgcg ccatcatttg cttaaatttt ccgggcaatt gtggcacttc
    80101 gagcagcata aacagcaccg tcagcagcaa taaaaatatt gatgacatgg cattagataa
    80161 ctgcgtcaat aagttggtga gcaacgtcat cgccgcgttc ggatcaatgt aatgcgccag
    80221 ttggtcaact gagacgtcaa tccctacgcg ttgcaacaac ggctcaagag cttgcagcgg
    80281 cgtcataata gagttgcgat attgcggtaa cgtccgcgtc aactcgttga gcgtggaacc
    80341 cagataagct aacagcaaca ccatagccat cacgatgatg gtcatcaaaa tcgacactgc
    80401 cagtacacgc ggcacacgcc agcggaccat gtgttgcacc agcgggttaa gaataacagc
    80461 aataaataat gcgagaataa acggcacgat gatctcggcg gcaaaacgga taccgcagag
    80521 aataatgacc agcattccca acataatgac gatttttagg ccattgagcg tgatgagcgg
    80581 ctttgccatg cttgtccccg ttttttcttc ctttgctaat aataatgtct ttttcgctcc
    80641 gtcatcataa cgaaaatcaa accgctcagg taaagaagtg aaaatatttt gagttaattc
    80701 ttaagctatg atacaaatca ggcgtgttca actaccgagg acaattatca tccgcgatga
    80761 cgagaagcaa cactgcggat aattgtaata ttatggacaa tttgttcagg acgttatttt
    80821 ctacatttac ccatcttcgt acctcatcca ctatcttgct cgtaggtgag cagcactggc
    80881 gcaacgcgct ttagtccttt cttctgccta agcttgccct atacgcgggg cttgaccact
    80941 ttactttggt ttcgctgaat taagcgaaaa ttaaaataat tctcttgcag gagaaggaca
    81001 atgtatattt attggatttt attaggtctg gctattgcta cagaaattac cggtacgctg
    81061 tctatgaaat gggcgagcgt cagtgaggga aatggcggct ttattttaat gctggtgatg
    81121 atttctctgt cgtatatatt tctctctttc gctgttaaaa aaatagcctt aggtgtggct
    81181 tatgcgctgt gggaaggtat cggtatttta tttattacct tgtttagcgt tttgttattc
    81241 gacgaaagtt tatcgctgat gaaaattgcc gggttaacca ccctggtcgc cgggatagtg
    81301 ttgatcaaat caggtacccg taaagcacgt aaacctgaac tggaggtgaa ccatggcgca
    81361 gtttgaatgg gttcacgccg cctggctggc attggcaatc gtgctggaaa tcgttgctaa
    81421 cgtctttttg aaattttctg acggctttcg tcgcaaaata tttggcttgc tctccctggc
    81481 ggcggtgctg gctgccttta gtgcgctttc tcaggccgtt aaagggatcg acttgtctgt
    81541 cgcttatgca ttgtggggcg ggtttggtat tgccgccacg ttagccgcag gttggatctt
    81601 gtttggtcaa cggttaaatc gtaaaggctg gattggcctg gtcttgctgt tggctggaat
    81661 gatcatggtg aaacttgcct gatgaagacg ctgcccgcgc tgtcgggcag cgtttgaaca
    81721 ttatttttgc gacagttgat ccagcttgtc gcgaaaaccg gtaacagaaa tggcccggtt
    81781 atcggcgcgc cagcgatctt tcgcggcagg agccgaactt tgcaccccaa ttaattgcca
    81841 gccgtcatcg gtatgcaaca tcagaggcga accgctgtca cccggcaagg tatcgcactg
    81901 atgtgacatc accgacgttt gcgcccagcc agtcacttca cagttttgat gactgtacaa
    81961 cgtatcgaga tgatcttcag ggtatcctgc ctgagtcact ttacgacctg ccgcttttaa
    82021 tgcggcggta agcgcggctt tatctccctc aaataacggc aacggcgtaa tgccagaagg
    82081 gggattacgt agcacaatca atccgaagtc ccacggcgcg gctgcgggag gtacaatcca
    82141 accatcccca tctgctttta accgctttcc cagtgtcgga tcaacgcggc cttctatgtc
    82201 gtggatgtca tagcgccaaa gacctttatt tgacacaaaa cgcagcgcca ctgctttatc
    82261 ggctttaccc tttggtggcg tcaataaaca gtgtcctgcc gttaatgcca gattgggtgc
    82321 aatcagcgtc gccgtacata aattgccgct ggccgtttcc agttgcccaa ccgcatccca
    82381 cggtgattgg gtcgtgtcat tcactggcac acgatcatca tgaccaaaaa acagggtgct
    82441 gacctcatcg tttgccgatt tggcaacgtc tggtttatct gcaaacacaa aagcagacgt
    82501 caaactaatt gcacccaaca ctacagcaat ggttgtacgc atatcacact ctggtgggta
    82561 attatgatta ttaaaagcaa accctcataa atactataga cgggacggga tgaaagtggg
    82621 agtaaaatca gataactacg atcaggaaag ataaaaacgg gcggcaatca gtgcgcataa
    82681 aataaggatg ataagaatga actcaaagcg ataacggccc accatactac cctccgctaa
    82741 aggcggtgct aaacgttacc cgcttagcac cgtaattttc aggcgcgagg gggcgcgcca
    82801 gcattactgt tgaaaactta cgctgcgggt tgtgcagcag gttttgccgg ttgctgatgg
    82861 ctgtgtttct tggcgtgttt cttcgctgcc tgcgcttttt gttctggggc tttctgttca
    82921 gctttcgtat ttttatgatg ctttttagcc gcctgcgctt tctgggcagg tgctgcttta
    82981 tgctgttttt tatgatgtgt agttttcgcc ggcgctgctt tggtggtcgt cgcagtcgga
    83041 gcaggtgtgg tcgcagtctc tgcagcaaag gcggcagaag acagacccat agcagcggca
    83101 acaaccagag ctaatacttt tttcattgtc ataccctcaa tttgtttttt catttaaccc
    83161 cactgcgggg ccgttgaaag aactatatcc ctgggaatgg cagacttccg tgagtggttg
    83221 gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt acagcgttga taataacgta
    83281 ttgtgactta aggaaaattt agctaccaat aatagtagtc ttgatcgggt taactatttt
    83341 actacttaca aagcggtaga ataactgcgc atcagtaata aatgacacac agcaaaatga
    83401 atccgtttat ttgggtactt ataatcctga tgacgctaga cgcgctgcgg gaattggctg
    83461 gcgcttcgtc tattttagga tggctattaa cgttggtttg agcaggcaat aagtccggac
    83521 gggtatttac cgcagtccgg acttattttt caggcgtgca gacgacgatg caaacgcgtc
    83581 ccgaccagca gagcaatgac cagcatcaga gcaataaatg cgccgacgcc gttccagcca
    83641 tagttatgcc agaaaacacc acccagcgtc ccggcaatac tcgaccccag atagtaactg
    83701 aacagataca gcgaggaggc ctggccttta gcgcgttttg cgcgggggcc gatccagctg
    83761 ctggcaactg agtgggctgc gaagaatcct gctgagaaga gtaacattcc ggcaaagatc
    83821 agccacagcg agctgaataa ggtcatcagt aaaccaaaca gcataacccc cgtcgaaaac
    83881 aacatcactg gaccacgccc atagcgggtg gtcatggttc cggctttggg tgagctccat
    83941 gtaccggtca aataagccag cgataataag ccaaccacgg cctgactgac atgccagggg
    84001 gagagcatca accgatagcc gatgtaatta aacagcgtga cgaacgaccc catcagcaaa
    84061 aagccttctg cgaacaataa cggtaatccc cggtcacgcc agtgcagacg aaagttgata
    84121 aacaacgact taggtcgcag cgaagtcggg cgaaaatggc gtgattccgg gaggattttc
    84181 cagaacatca acgccgaggc tagcgcgaaa caaccgatag ccgccagagc aattcgccag
    84241 ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc tcatgccgcc aattgagttg
    84301 ccgctgatat acaaccccat cgaaaaggcc acgaaactgg ggtggatttc ctcgctaaga
    84361 taagtcatgc caacagctgc cacgccactt aacgaaagcc caatcaaggc gcgcataatc
    84421 aaaatgccgt gccagctggt catcattgtc gaaagtaacg tacaaatgga ggccaatagt
    84481 agcgccgtta ccatcactgg tttgcgacca atggcatcgg atagggggcc agtaaacagc
    84541 aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa tactactgtt cgcgggcgtt
    84601 aagccaaact cctgcgaaag caccggaagg ataggttgca cacaatagag aagtgcaaat
    84661 gttgccagtc cggcagagaa cagcgccagg gtgacgcgca taaattgcgg cgtaccgcgt
    84721 ttaataaatt gatttggctg agaaatgctt tgcttgtcag tgtcgcttgc cggagcgcca
    84781 tcaacagttg tagtacggct cacttgaaat ccttgctaaa tatgcctgtg gatcaggctt
    84841 atacataggt taggaaaatc gaattgttct gtctaatata ttaataatct caaataagat
    84901 gttttaaata tgaatattga acttcgtcat ctgcgttact ttgttgctgt tgcggaagag
    84961 ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc aaccgccgct aagtcagcag
    85021 attcaggcgc tggagcaaca aattggtgcc cgactgctgg cacgaaccaa tcgcagtgta
    85081 ttgctgacgg cagcaggaaa acagtttctt gcagatagtc ggcaaatcct gtctatggtg
    85141 gatgacgctg ccgctcgcgc tgaaagactg catcagggtg aagcggggga gttgcgcatt
    85201 ggttttactt cgtcggctcc ttttattcgg gcggtgtccg atacgttatc gctgtttcgc
    85261 cgagattatc ccgatgtcca tttacaaacc cgcgaaatga acactcgcga gcaaatcgct
    85321 ccgctcattg aaggaacgct ggatatggga ttgctgcgta acacagcgtt accggagacg
    85381 cttgaacacg cagtcatcgt ccatgaaccg cttatggcga tgatcccgca cgatcatccc
    85441 ctggcaaata acccgaatgt aacgctggct gaactggcga aagaaccctt tgtctttttt
    85501 gatccgcacg tcgggacagg gctgtatgac gatattctcg ggctgatgcg acgttaccat
    85561 ttgacgcccg tcatcactca ggaggtgggc gaggcaatga ccatcatcgg tctggtttcc
    85621 gccggtctgg gtgtttcaat tttgcctgcg tcatttaaac gtgttcagct caacgaaatg
    85681 cgctgggtgc cgattgctga agaggatgcg gtttctgaaa tgtggttggt ctggccgaaa
    85741 catcatgaac aaagtccagc tgcgcgtaac tttcgtattc atctgctgaa tgctctcagg
    85801 tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt taatcacatg cctaagtaaa
    85861 aatttgacga cacgtattga agtgcttcac catagcctac agattatttc ggagcgcgaa
    85921 aatataggga gtatgcggtg gttgctgaaa accagcctgg gcacattgat caaataaagc
    85981 agaccaacgc gggcgcggtt tatcgcctga ttgatcagct tggtccagtc tcgcgtatcg
    86041 atctttcccg tctggcgcag ctggctcctg ccagtatcac taaaattgtc cgtgagatgc
    86101 tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc ggggaaccgt ggccgtccgg
    86161 cggtggggct ggtggttgaa actgaagcct ggcactatct ttctctgcgc attagtcgcg
    86221 gggagatttt ccttgctctg cgcgatctga gcagcaaact ggtggtggaa gagtcgcagg
    86281 aactggcgtt aaaagatgac tcgccattgc tggatcgtat catttcccat atcgatcagt
    86341 tttttatccg ccaccagaaa aaacttgagc gtctaacttc gattgccata accctgccgg
    86401 gaattattga tacggaaaat ggcattgtac atcgcatgcc gttctacgag gatgtaaaag
    86461 agatgccgct cggcgaggct ctggagcagc ataccggcgt tccggtttat attcagcatg
    86521 atatcagcgc atggacgatg gcagaggcct tgtttggtgc ctcacgcggg gcgcgcgatg
    86581 tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt cattaccgat ggtcatctgc
    86641 tacacgcagg cagcagtagc ctcgtggaaa taggtcacac acaggtagac ccatatggta
    86701 aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat cgccagtgtg gacagtattc
    86761 ttgagctggc acagctgcgt ctcaatcaat ccatgagctc gatgttacat gggcaaccgt
    86821 taaccgtgga ctcattgtgt caggcggcat tgcgcggcga tctgctggca aaagacatca
    86881 ttaccggggt gggcgcgcat gtagggcgca ttcttgccat catggtgaat ttatttaacc
    86941 cacaaaaaat actgattggc tcaccgttaa gtaaagcggc agatatcctc ttcccggtca
    87001 tctcagatag catccgtcag caggcccttc ctgcgtatag tcagcacatt agcgttgaga
    87061 gtactcagtt ttctaaccag ggcacgatgg caggggctgc gctggtaaaa gacgcgatgt
    87121 ataacggttc tttgttgatt cgtctgttgc agggttaaca ttttttaact gttctaccaa
    87181 aatttgcgct atctcaattt gggccaggaa agcataactt agactttcaa ggttaattat
    87241 tttcctggtt tatatttgtg aagcataacg gtggagttag tgatgctgaa gcgtttcttt
    87301 attaccggta cagacacttc tgtagggaaa acggtggttt cccgcgcatt gctacaagcg
    87361 ttagcctccc agggaaaaac ggttgcggga tataaacccg tagcgaaggg gagcaaagag
    87421 acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc agagtgtttc aaccatcgaa
    87481 ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag aagaaagtag cgtggcgcac
    87541 agttgcccaa tcaattacac cctcatttca aacggcctgg caaacctgac cgaaaaagtc
    87601 gatcatgtcg tggtagaagg gactggcggc tggcgtagtc tgatgaatga tttgcgtcca
    87661 ctctctgaat gggtggtgca ggaacaactg ccggtgttga tggttgtcgg tattcaggaa
    87721 ggttgcatta accatgcact gctaacagct caggcgattg ccaacgacgg gctgccgctc
    87781 attggctggg tggctaaccg aatcaaccca ggactggcgc attatgcgga aatcattgat
    87841 gtgctcggta aaaaacttcc ggcaccgctc attggtgaac tgccttatct gccgcgcgcc
    87901 gaacagcgtg aactggggca atacatccgc ttagctatgc tgcgcagtgt gctggcggta
    87961 gatagagtca cggtgtaacg tccgcgagat taccgacgca attacgcagg caatcaataa
    88021 accggggagt agctgatact ccccggtcat ttcacatatc atcaacgtcg acataatcgg
    88081 cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc aatccgagta aaagcgtaat
    88141 ttcttcgcca tcagggaacc ataatcccag actacgacca tacaacatgc caatggcaag
    88201 accgataaat agcgtcggtg taaagacacc accgggtgcg ccggaaccgc tactcgccag
    88261 cacggcacac agtttacaaa ggaagatccc ggcaatgatc attaacagtg gcggggcggt
    88321 taaaaaggat tgtacggtgc tatagccgtt gccccacact gcaggtgtga acagggaaag
    88381 cagacccacg atcaacccgc ctagtgccag ttgccagggc ggcgcaagtt tgagactcac
    88441 aaatccacga tgacaggcgt tcattaacgt taacaacagt ggtccgcaca gacctgccag
    88501 cacacctgta ctgataatca gcgcatagtc acgagcctga accgtcactg agagttgtac
    88561 gctgtagagt aacgcgtcgc tatgattaat cagattgcta accagcaatg ccacgacggc
    88621 ggaaataatc accgggccga gagaggccaa catcatagtg ccaaacagca cttcggctat
    88681 aaataaactg ccagcaagcg gggcacgata ggccgcagcc attcccgccg cggccccaca
    88741 ggcgatccat aatttccact cctggcgtgg cgtaaaacgt tgggcaaaac aggaggcggc
    88801 aagggcagct aaaagaatca tcgcaccttc gcgaccaatt gcactgccgc tggttactac
    88861 cagcagagag gcaagcgatt taaccaggct tgctgcgtaa tcaaactgac catcggtttg
    88921 caacgcttcc atgtaatcgg tcggcgcatg agggcgttgc tgggtaaatt tctgccagcc
    88981 catcaacaac aaacccgccg ccagtccgcc gagcgccgga gttagcagcc gtcgccaggg
    89041 ggaaaggttt gtcgctgcat tgaccagact gccggagtca ttattgagga acaaccactc
    89101 cagtagcagc atcgcatgac gaaacccggc aacggcaaag gccgcgagaa taccgacgac
    89161 tgttgcgata agcagacggc ggaacatagt gcgcagatcc ggataggtgt gtagatgatg
    89221 catgagcaga aatgggcaat gaaacacggg cgactatttt gcggcaaaag aggccagctg
    89281 caaaagcagt gcgtgcttta agagacaaaa aacgtgacac tgtaacgtat tgttgccgaa
    89341 tcgcgcgtaa tcgtcaccat ccggcaatat tacggtgatc ctatcgaaag agtggtttaa
    89401 ccgcgacagg aattaacagt tgtgattgcc attgcgccag cgttaagcga gccagttcac
    89461 ccagtgcacg gtagaaaggg tgttctgctt tttcgataaa aacgtcaaga aaacgtgttg
    89521 accagggaaa aaggtgccat gccagcaatt cttcgcattc tgtctggcga ccattctccg
    89581 ccaaccacgc agccatcaac agcagtgaac caaaatgatc ttccggttcg ttttgcttca
    89641 tttcaaactg aatgcctttc tcgcgcatcc actgacgaag tgccaatgtt gaatcgccaa
    89701 acagcacaga ttcgcgatcc agccagaccg acccccacgg cggagacggc agtgcccacg
    89761 ggccgacaaa caaacgctgc caggcctggg cgtgagtctc ttcacactgt gtctgaaaag
    89821 ccgtcaccag cggcgctaat gacgcttctg gtagaggcca ctgagtttcc cagccgtcac
    89881 tggtgagtac cgcaaccaga ggtgcggctt cagcactctc tggagcgtaa taaaacagcg
    89941 cgcccaacac gcgcgccgcg acagaaaaat tatcttgctg tgaaaaatgg gtcattccta
    90001 acatcctgaa atgtgcgggt tgccccgcac caatttgtta acctgcaata gccattccta
    90061 ccgtcatgtg caggccataa aagagcacac gcccgatcat ctcaccgcca agaatgagga
    90121 ttaaccccag aataagtccg gcaacgtgag gttcacggcg acgtatgagc ggacaaagcc
    90181 agcatcctag acctgcgcat aacaatacca cacgccagac ctgtaaggag gcgtaatctg
    90241 gtaccagtgc gctggcttgt tgcacggatg agtgaataga cgacagagac aaaccttgca
    90301 gcacaatcac cccggcgcaa gcgatcagcg ccagaacgct aatgatggca aatggtgtgg
    90361 tattaaatgt aacgcgggct gcccgcagaa ttgcggcagc aagtattgga ccactcaaca
    90421 ataccgtcac gaagaaagcc agcgtagtgt aaccgttatg ccaggttggc acagtgtcga
    90481 tttgatacac gcaggtcatc atccagacga aaatgacgcc gagcgccatg ctgacaagta
    90541 accagagttt ccccaacgct tgcggcattt taccgatgac cgccaccagc caccatagtc
    90601 cgccaacggc aaaaaatatg gaaccggcag caatctcgtt actcagacca gaagctccga
    90661 ttcgattcag cgagttaaac gcccgcagtg gcgatcccag gtgcatgaca gaggcaataa
    90721 acccgatgcc catcaacagc cagaggaaaa acatgccacg aacaatgtgc tgacggtctg
    90781 cgtcagtttt ggcggcgaac cagccaattc cgctaacaat cagcgcgcct actacgcatt
    90841 gacccaatac ggtaaagata accagtggcc attcatgcca tccatttccc attttacact
    90901 tcctccggat tagccagata accagtggta tcgccggtcg ggcggctgtt ggcgttaggt
    90961 ttgataacga tattgggttt tgtgaaatgc gcgcgcggca gtggcgcgac ggcagcaaga
    91021 gtgccgtgct tctggcgcag ctcttcaatt ggaccgaact caagtgcacg cagcgggcag
    91081 gattcgacac atatgggttg tttcccctcg gcgacgcgcg aataacaacc atcgcacttc
    91141 gtcatgtgcc ctttttcagc attgtactgt ggcgcgccgt acggacaagc catgtggcag
    91201 tagcggcagc cgatacaaac atcttcatca accaccacaa agccatcttc acgcttatgc
    91261 attgcgccgc tggggcagac ttttgtacat gcggggtcat cgcaatggtt acaggagatg
    91321 gagagataat aagcgaacac gttctggtgc cagacgccat tatcttcctg ccagtcgccg
    91381 ccagcatatt cataaatacg gcggaaactg acttccgggc caagatcttt aaaatctttg
    91441 cacgccagtt cgcaggtttt acagccagta cagcggctgg aatcgataaa aaatccatat
    91501 tgtgtggtca tgggctactc cttaaacctt ttcgatctgg acaagattgc tgtgcgacgg
    91561 gtttcccttt gccagcggtg aagggcggtg agaggtcaga atattgatac tgccgccgtg
    91621 atcgacccgg tcaccaaaca tatccgcttt aagccacgca ccttgcccga tggcggtaac
    91681 gccaggcaga atacgcggag tcacttttgc ggcaatcagc atttctccat tattgttaaa
    91741 tacccgcacg gtatcgccat gacggatacc gcgagcctga gcatcaatgg ggttgatcca
    91801 cacctcttgt gggcaggcct gctgtaacac atcaatattg ccgtagctgg agtgggtacg
    91861 cgctttgtag tggaagcccg ttaactgcag tggataggtt ttccgcaggg gatcgtccca
    91921 gccatcaaaa cctggggtat acgcaggaag gggatgaata atttcatctt ttttcaattc
    91981 ccaggtatct gcaatcttcg ccagtcgttc agaataaatt tcgattttcc ccgaaggtgt
    92041 tttcaacggg tttgcctgtg gatcttcacg gaatgcgcgg aaagcgacgt agtgttcttc
    92101 cgggcatttt ttcttaaaga tcccggtcgt tttcatctcc tcgtagtcgg gcatctcagg
    92161 gttacgttcc ttcgttttcg catggagata tttgatccat tcatgctgac tgcgaccttc
    92221 agtaaaggtt tgataaacgt ccggtcctaa gcgtttggcg acttcactca gcatccagta
    92281 gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg gcgaggatca catagcccat
    92341 attccctgca gattcatgag agataaggtc ttcttgctct gttggcatca ggtcgggcaa
    92401 caggatatcg caatacttag ccgaggccgt catgaagtgg tcaatgccaa caatcatctc
    92461 gcacttgctg tcatcctgaa gcacctcatt ggtgtgattg atgtcgccat gttgattgat
    92521 caatgtgtta ctggcgtagc accataaaaa cttgatgggg acatccagtt tttcttttcc
    92581 acgaacacca tcacgggtcg cggtcatttc cgtaccatgg tcgatggcat ctgtccatgt
    92641 aaagacggaa atctgcgttt taacaggatt ctcgagcatc gggaaccatt ctacccccag
    92701 atcccagcta ccttcgcgta cacctgagtt gccgccgttt atgccgacgt taccggtgag
    92761 aacggaaagc atggcaatag cgcgggatgt ttgctcgccg ttggaatgtc gttgtggccc
    92821 ccaaccctga caaatataag caggttttgc tgaaccgatc tctcgtgcca actggataat
    92881 tttttctgcc gggatgctgg tgatttttgc tgcccattcc ggcgttttag ctatgccgtc
    92941 aggcccttcg cccagaatat aggctttata atgcgcgtta cgtggtgcgt tggcgggcag
    93001 cgttttttca tcgtaaccaa cacaatattt gtcgagaaat ggctgatcga ccatgttttc
    93061 actaatcagt acccaggcaa tcgcacaggc cagtgcgcca tcggtgccag ggcgaatggg
    93121 cagccattca tcttcacgcc cggcagctgt gtcgttataa cgtggatcga tgacgatcat
    93181 gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa gtgacaccac caccgctcat
    93241 ccgcgtttct gccgggttat ttccgaacat aacgaccagt ttcgtattgg cgatatcatc
    93301 cgggctgttg ccatcattgg caccgaacat ataactcatt gcggcactga tctgtgcggt
    93361 actgtagctg ccatagcgac tgagaaaacc accgcaagag ttcatcagac ggtacgggac
    93421 gtttgagttg gtgatgtttc cgccatctac gcctgttccg tacaggacat gtacagcctc
    93481 attgccgtaa tctttcagga tccgccgaag attatcactg atggtatcca gggcttcgtc
    93541 ccaacttatc cgttcaaatt taccttcacc gcgcttgccg acgcgcttca tgggatattt
    93601 caacctatca ggatgattca tccgtcggcg gatagagcgc ccgcgtaaac acgcacgaac
    93661 ctgatgatta ccgtagacgt cgtcacctgt cgtatcagac tccacccagt acacggtgtc
    93721 atctttcaca tgcaaacgta acagacagcg gctcccgcag ttaacggtgc aggaactcca
    93781 gaccgctttc tcttctaccg gtgcctctgc cgctcggacc atttgggaaa atggcagagt
    93841 gaaagcactg cttgccagcg caagactgcc aagtgcggag gttttcatca gacttctacg
    93901 gctgatttca gccttcatga gcgcctctgt ggtatggatt ttcatcatta ctcacttatt
    93961 gcttttcaaa caaaatgtca tgccagaatt tatggttgta gtgggttata ttttttcgat
    94021 ctcgaccaga ttcgtgtgct gcgggtttcc cttcgccagt ggtgaagggc gcagagtggt
    94081 tagcgtattc acacatccgc catggtcgat tttatcgcca gacatattgg cctcgtgcca
    94141 ggctccctgg cccatagcgc taaccccagg gagaatgcgt ggtgtcactt tggctggtag
    94201 ccgaacttcg ccacgatggt tatacacccg caccatatcg ccgttggcaa tcccacgttt
    94261 ctgtgcgtct atagggttga tccacacctc ctgacggcag gcagccttca ggacatcaat
    94321 gttgccgtag gtcgagtgag tacgggattt gtaatggaaa ccaaacagtt gcagtggaaa
    94381 ggtactacgt tcaggggagt cccagccttc aaaggttgag gcataaactg gcagtgggct
    94441 tatcacttca tctttttcca gttcccaggt acgggcaatt tccgccagct tgctggaata
    94501 aatttcaatc ttaccggaag gtgttttaag tggatttgcc tcggggtcgt cacgaaatgc
    94561 tttgtaggcg acaaaatgac cattgggatc tttacgctta tagataccca tttttttcag
    94621 ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt ttggcgtaca gatgttgtaa
    94681 ccattgttcc tgcgtgcgac cttcagtgaa cttttgatag acgtcaggtc caagacgttt
    94741 cgcgacttca ctcaggatcc agtaaatcgg tttgcgttcg aatttttcgc tggtgacagg
    94801 ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg ttaggaataa tatcttcctg
    94861 ctcaacagtc atcaggtctg gcagcagaat gtcggcatat tttgccgatg aggtcataaa
    94921 gttttcgatg accacaatca tttcgcattt cgattcgtcc tgcagaattt catgcgtttt
    94981 gttgatgtca gaatgctgat taaccagggt atttcccgcg tagttccaga tgaacttaat
    95041 gggcacatcc agtttatctt tgccccggac accgtcgcgg attgccgtca tttgtgggcc
    95101 atgatcgata gcatctgtcc agctgaagca ggaaattgac gttttgaccg gattatccag
    95161 caccggcagg cgctctatgg taatggtata ggtcgattca cgcgcgccac tatttccgcc
    95221 gctgatgccg acattgcccg tcagaatagg caacatagca atagcgcgtg cggtcagctc
    95281 gccgtttgcc tggcgttgcg gcccccagcc ctggcagata taagcgggtt ttgctgtgcc
    95341 aatttcacgc gccagtttga tgatacggtc caccgggata ccggtaattt gcgaagccca
    95401 ctgcggcgtt ttcgctgttt tatcgtcacc ttcaccaaga atataggctt tatagtgacc
    95461 atttttgggt gcatctgcgg gtaaggtttt ttcgtcatag ccgacgcagt atttatcgag
    95521 aaaaggttga tcaacgagat tttcgttaat caatacccag gcaatacccg caaccagcgc
    95581 ggcatcggtg cccgggcgaa taggaagcca ttcatcttca cgaccagcag ccgtatcggt
    95641 atatcgcgga tcgataacaa tcatttttgc gttcgatttt tcgcgggatt tttcaagaag
    95701 ataagtgatg ccaccgccgc tcatgcgggt ttctgccggg ttgttaccaa acatcacgac
    95761 cagcttgctg ttttcaatat ccgtggtgct gttgccatca ttactgccgt aggtgtaggg
    95821 catggcacag gaaatttgcg cagtgctgta ggagccatac tggttgagtg aaccgccgta
    95881 gcagttcatc aggcgtttga ccgccgaggc tgatggcgaa gagcgggtta tattgccgcc
    95941 aacgatcccc gaagagtact gaatatatac agcctcattg ccatattgtt cgacggtttt
    96001 cttcaggcta ctggcgatag tatccagggc ttcatcccag ctaatccgtt cgaatttgcc
    96061 ttcaccgcgt ttacccacgc gtttcattgg gtaattcaag cgatcgggat gattaatacg
    96121 ccggcggatg gagcgaccgc gcaaacaggc gcgtacctga tggttgccat actcatcgct
    96181 gccggtgttg tcagtttcca cccaggccac ttcattatct ttaacatgta gacgaagtgc
    96241 acagcggcta ccacagttga cggaacaggc accccagacc acttgttcgc tggcctgttg
    96301 tactgctgcc gctgcactgc gcagggtgaa cggcaaagaa aaaccgcctg cagccagcgc
    96361 cagagaacct atcgcggtag atttaacgag tgttctgcgg ctgatgccca ccattcgttc
    96421 atttttggac ataactcact ccctgttctt tatcgttata taaaagttta tatattgaat
    96481 atttagcgcg ctaacaatag agggagtcta cccattttgg gttaataatt attaatccat
    96541 atcaatagaa gggtatgagt aatatggtgg gattatgttg tatgtccaaa tcgccggatg
    96601 tgtcgtatcc ggcgtgtagt cgataatgta ttactgcggt tcggcaggcg cgccatcctg
    96661 ggtagactgc gcgggagcag agacgttacc gctggtggtg cgggtgtaga gaattttatg
    96721 cgtatcatta gcgcaatgcc cgacgacctg ggaatcaggc tgatcaactt ggtcattggg
    96781 tacaatactt aacgtgaagc tgctttcggg tacgccatta ttgataatgc gctgtgatat
    96841 atcgctttgt atgcgctcac aggatcccgg cgcggcgagt accgcgggtg aggcgagggc
    96901 gagcagaagc gcggcataac aggttgagag tttcatcata agctcctaac gcgaagataa
    96961 cttctttaag catagcattt cacgtgtaaa gtactgtatt tgctactatg attgagaatc
    97021 atctctactc tctggtgact gatgtgaaat acaaattact gccatgctta ctcgcgatat
    97081 tactcacagg atgtgaccgc acagaggtaa cactttcatt tactcctgag atggccagtt
    97141 tctctaatga attcgatttt gatccgctgc gtggtccggt aaaagatttc actcagacat
    97201 taatggatga gcaaggtgaa gtgacgaaac gtgtttcggg gactttgtcg gaagaaggct
    97261 gttttgattc actcgaatta ctggatctgg aaaataatac cgtggtcgct ctggtactgg
    97321 acgccaatta ttaccgtgat gccgagacgc tggagaagag agtacgttta cagggaaaat
    97381 gccagctagc agaattacct tctgccgggg tgagttggga aaccgatgat aatggcttcg
    97441 tgattaaagc cagcagcaaa caaatgcaga tggaatatcg ctatgatgat cagggttatc
    97501 cactgggtaa aaccacgaaa agtaacgaca aaacattatc tgtcagcgcc acgccatcaa
    97561 cggacccgat caaaaaatta gattacacag cggttacttt actgaataat caacgagttg
    97621 gtaatgtaaa acagagctgt gaatatgaca gtcacgccaa tccggtgggc tgtcagctga
    97681 tcattgttga tgaaggagta aaacccgccg tcgaacgggt ttacaccatc aaaaatacga
    97741 tcgattatta ttaatgctat tgtgcggtcg gcttcaggag agtctgaccc ggtgttttgt
    97801 gctctgccag atactgatgc tggaatatac acatgcgaat ggcattacga tattgaccat
    97861 taataaagaa ctcgtgcatc aattcacctt caaccgaaaa gccaagcttg cggtaaatgt
    97921 gaatcgcttt ttcattctct ttatcaacga tcagatacag cttatagaga ttgagaacgg
    97981 tgaagccata gtccattgct aatttggcgg cacgggttgc cagacctttc ccctgatact
    98041 ccggggagat aattatctga aattctgcgc ggcgatgtac atggttaatt tccaccaact
    98101 ccaccagacc ggctttttcg ccgtcacatt ccaccacaaa gcgtcgttcg ctctgatcgt
    98161 gaatatgctt atcatacaga tcagagagtt caacaaaggc ttcgtagggt tcctcaaacc
    98221 agtaacgcat cacactggcg ttattgtcga gttgatgtac atagcgtaaa tcttcacgct
    98281 ccagcgggcg tagcttaaca ctgtgggcgc ttggcataac gtgtccttac attccttaaa
    98341 tcaataacag gttagggggt aataacgcgg ccagttcgac ggtccaggca gcgcaaagta
    98401 tttggctccc agtaggcatt gatgttggcg ctttgctcgc atttatcgcg gttatcaaaa
    98461 gcggcgtcgg ctttatccca ctctttttca gtgcgtttat tcactttctg gcgcagattg
    98521 cccgtgtcat tccattgctc tttttccata gcggcgtgct ggcggctttg tgcactgtcg
    98581 ccagactcaa tcaccagttt gttagtttcg gcatgaacag ttgtgctcaa tgccagtgcg
    98641 caaggcagca gaaaagcgag caggtcgatt cgtttgctga gagtgatttt cataattcat
    98701 tccctgtatg aatgattaaa gatgattcta caccatccac tgcgaacgca aaacgtacca
    98761 ggagggtgtt tatattgatg atattatgtc gccctataac tatacatgat gtcaataagt
    98821 gacaaagatg attaaaacaa cgttactatt ttttgctact gcgctgtgtg aaattattgg
    98881 atgctttctg ccctggttgt ggttaaaacg aaacgccagt atctggctgt tgcttccggc
    98941 ggggatttca ctggcgctgt ttgtctggtt gttaacgttg catcctgcgg cgagtgggcg
    99001 tgtttacgcg gcttatggtg gcgtttatgt ctgcacggcg ttgatgtggc tgcgcgttgt
    99061 ggatggcgtg aaactgactc tttatgactg gacggggccg ttgattgcgc tttgcggcat
    99121 gttgatcatt gttgtgggct gggggcgcac gtaggaacat aaatccattt tatcaataag
    99181 atacgaggaa gtgtcagctg acaaaaggta ttctatttca tcttttgtca accattcaca
    99241 gcgcaaatat acgccttttt ttgtgatcac tccggctttt ttcgatcttc atacttatat
    99301 ggtagtagct cagttgcgta gatttcatgc atcacgacaa gcgatgcaag gaatcgaaca
    99361 tgaagatcgt aaaggctgaa gtttttgtta cctgtccggg gcgtaatttc gtcacattaa
    99421 aaatcaccac tgaggacggt attacaccat aaatttttgg cttgctgatt tctgcacgca
    99481 tcgataagac ggatgaattc ctcccgcgaa agaggatccg gaatggttct tgattccttt
    99541 aatggcgaga tccccttaaa cgggttatct gccaggtaac cgttatcaac accaaactgg
    99601 aacacggcgt taagatttgt catgtaatta tttacagtta cagccgatct ccctggttgt
    99661 gtaacaatat agttactttt ggggatctgg tatccagtca gtagctcttt acgaacctcc
    99721 agtaattttt ctttattaat cgatgaggca agattttttt caccgattat gctcaggata
    99781 tttttgatga cggcacggta tgtgttgagt gatgtttttg cgacttcagt ttctttcagt
    99841 gccagaaatt tttcagccag ttcttttatg gttaaatctt gtcgggcctc accaaatttt
    99901 tccagattgc gtgaggaggg aaactgtttt gcatagtcga aaacaccagt ttttattgcg
    99961 taacaaacag aggcgcgtag ctcacctgca acgcgcctgt tttttgctgt gtcaggaacc
   100021 ccaggttttc cctgactctt acgcctttat aaacaaacca gatacgtaat ttccctccat
   100081 ggttttccac gcctgtcgga tatttcattt caacttctct cattagttag tgtggctttt
   100141 agtcaagtaa gatgacgtct tggtcttgct gatgcctggc gctcaatcca gcgatcaatt
   100201 tcttccaggt tgtaaaagca tggactgtta tcccatggca taccgtcatg agcgacatgc
   100261 ttatattccc ttccttccat aaacgatttt tcccgggcct tttttaacgt accttttttt
   100321 attcctttca gcgcaattaa ctgctcttcg gatacccatt tgccgggaga gacaatcatg
   100381 attacttcgc tcatcgattt ctttatctct tacatcagac gagcgccggt tgcagaatac
   100441 cagtcacaac cggcgacagt tgaacattaa gaatcagcct gactcgggat cagtttttgc
   100501 cagatagctg aaacgtattt tgcctggtaa cgggcgtcat caagtgcatt atggcgctca
   100561 ccttcgaatg gaatagccgt tctggcatcg aagtctatgg ctttccccag ctcaacgatt
   100621 gtgcgtacat cgcgatcgtt gtagtaacgc cacgggcagg ggctcccctg ccgttcgtat
   100681 gaacggcgca aaatcgtgtt gtcgaagttg gctccatttc cccagacatg aacaaaaaat
   100741 tcaccggagt tttcgtcgat aaattcccgc aattgtaaca gtgcatcatc taacgggatt
   100801 tcatcggtca taatggcaga ttgcgcttcg cgtgattgtt taagccacca tttcatggtg
   100861 tcccgatcaa tgactccgcc agcagtatcc agatcgatag tcttactaaa ttccggtccc
   100921 atatctccgg tttgcggatc gaaaaatatt gcacctattg agataatcgg ggcatcggga
   100981 ttttttccca tggtttcaag atcgatcatc agatgaatcc ccgctctgct ggtggatgtg
   101041 agattatgat gaccgttcgc cttaattaag ggatctgacg cctcgccagt ttcactatcg
   101101 ctggcatgat gctgattgcc gccagtgttc tccttgtgct gatgtgcagt accttccatt
   101161 tcctccggat cattttcctg aacttcaggc tgattctctc catcgaatat ttcctggtat
   101221 gttgcgtcac ccatcaccgc accacaatca gggcagttgc cgccaccgct ctgaccgcag
   101281 gcggtgcaga tcttttccgg ttcctgttgc actactggtt caggttgttt cgtttctggc
   101341 tcgttttgtt gcgtatttgg gctgttttgt tccgctttct ggtcgttctg ttccgtttct
   101401 tgctggttct ggttcacaga atcgcgggtc tggatcccct taacccattt cggatcattc
   101461 gggtcgctaa tcccttcaac aaattcacca cgtgatactg caagcagttc ctcggcgtca
   101521 ggctggctga tattggctgc ctgcataatt ttctttactt cgtcagcggt aacttttacc
   101581 ggttctggtt gttcagaatt ttgtgcggta tttgcatttt gcggtaagcc tgtgtatgtg
   101641 ccattttttc gggcaaaata ttcttctttt gtgatttcag tagccccggc agccagcgcc
   101701 ttatccagac cagaaagttt gtttgcgcga ccgtattttt cgccatcctt gtcggtgaag
   101761 aggaagtaga acggcccctc acgttctaca gatggttcga cttccacttt gcattcggtt
   101821 ttttcgttgt ctggaattgc cgtttccact gcatcag
//
LOCUS       DABSYT020000006       100313 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.006, whole genome shotgun sequence.
ACCESSION   DABSYT020000006 DABSYT020000000
VERSION     DABSYT020000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 100313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.006"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..513
                     /gene="yfgH"
                     /locus_tag="ID725_RS02935"
                     /old_locus_tag="pgaptmp_000587"
     CDS             <1..513
                     /gene="yfgH"
                     /locus_tag="ID725_RS02935"
                     /old_locus_tag="pgaptmp_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGV7849261.1"
                     /translation="KFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETK
                     TVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGG
                     AVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRI
                     QPNTKCPEKS"
     gene            529..1068
                     /gene="yfgI"
                     /locus_tag="ID725_RS02940"
                     /old_locus_tag="pgaptmp_000588"
     CDS             529..1068
                     /gene="yfgI"
                     /locus_tag="ID725_RS02940"
                     /old_locus_tag="pgaptmp_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGV7849262.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1161..2738)
                     /gene="guaA"
                     /locus_tag="ID725_RS02945"
                     /old_locus_tag="pgaptmp_000589"
     CDS             complement(1161..2738)
                     /gene="guaA"
                     /locus_tag="ID725_RS02945"
                     /old_locus_tag="pgaptmp_000589"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGV7849263.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2807..4273)
                     /gene="guaB"
                     /locus_tag="ID725_RS02950"
                     /old_locus_tag="pgaptmp_000590"
     CDS             complement(2807..4273)
                     /gene="guaB"
                     /locus_tag="ID725_RS02950"
                     /old_locus_tag="pgaptmp_000590"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGV7849264.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4435..5805
                     /gene="xseA"
                     /locus_tag="ID725_RS02955"
                     /old_locus_tag="pgaptmp_000591"
     CDS             4435..5805
                     /gene="xseA"
                     /locus_tag="ID725_RS02955"
                     /old_locus_tag="pgaptmp_000591"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGV7849265.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5802..6017)
                     /gene="yfgJ"
                     /locus_tag="ID725_RS02960"
                     /old_locus_tag="pgaptmp_000592"
     CDS             complement(5802..6017)
                     /gene="yfgJ"
                     /locus_tag="ID725_RS02960"
                     /old_locus_tag="pgaptmp_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGV7849266.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6086..7558)
                     /gene="der"
                     /locus_tag="ID725_RS02965"
                     /old_locus_tag="pgaptmp_000593"
     CDS             complement(6086..7558)
                     /gene="der"
                     /locus_tag="ID725_RS02965"
                     /old_locus_tag="pgaptmp_000593"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGV7849267.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7676..8854)
                     /gene="bamB"
                     /locus_tag="ID725_RS02970"
                     /old_locus_tag="pgaptmp_000594"
     CDS             complement(7676..8854)
                     /gene="bamB"
                     /locus_tag="ID725_RS02970"
                     /old_locus_tag="pgaptmp_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGV7849268.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8865..9485)
                     /gene="yfgM"
                     /locus_tag="ID725_RS02975"
                     /old_locus_tag="pgaptmp_000595"
     CDS             complement(8865..9485)
                     /gene="yfgM"
                     /locus_tag="ID725_RS02975"
                     /old_locus_tag="pgaptmp_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGV7849269.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9503..10777)
                     /gene="hisS"
                     /locus_tag="ID725_RS02980"
                     /old_locus_tag="pgaptmp_000596"
     CDS             complement(9503..10777)
                     /gene="hisS"
                     /locus_tag="ID725_RS02980"
                     /old_locus_tag="pgaptmp_000596"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGV7849270.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10888..12006)
                     /gene="ispG"
                     /locus_tag="ID725_RS02985"
                     /old_locus_tag="pgaptmp_000597"
                     /gene_synonym="gcpE"
     CDS             complement(10888..12006)
                     /gene="ispG"
                     /locus_tag="ID725_RS02985"
                     /old_locus_tag="pgaptmp_000597"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGV7849271.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12033..13046)
                     /gene="rodZ"
                     /locus_tag="ID725_RS02990"
                     /old_locus_tag="pgaptmp_000598"
     CDS             complement(12033..13046)
                     /gene="rodZ"
                     /locus_tag="ID725_RS02990"
                     /old_locus_tag="pgaptmp_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGV7849272.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13331..14485)
                     /gene="rlmN"
                     /locus_tag="ID725_RS02995"
                     /old_locus_tag="pgaptmp_000599"
     CDS             complement(13331..14485)
                     /gene="rlmN"
                     /locus_tag="ID725_RS02995"
                     /old_locus_tag="pgaptmp_000599"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGV7849273.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14635..15066)
                     /gene="ndk"
                     /locus_tag="ID725_RS03000"
                     /old_locus_tag="pgaptmp_000600"
     CDS             complement(14635..15066)
                     /gene="ndk"
                     /locus_tag="ID725_RS03000"
                     /old_locus_tag="pgaptmp_000600"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGV7849274.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAGHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15207..16061)
                     /locus_tag="ID725_RS03005"
                     /old_locus_tag="pgaptmp_000601"
     CDS             complement(15207..16061)
                     /locus_tag="ID725_RS03005"
                     /old_locus_tag="pgaptmp_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGV7849275.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16061..16882)
                     /locus_tag="ID725_RS03010"
                     /old_locus_tag="pgaptmp_000602"
     CDS             complement(16061..16882)
                     /locus_tag="ID725_RS03010"
                     /old_locus_tag="pgaptmp_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HGV7849276.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16875..17504)
                     /locus_tag="ID725_RS03015"
                     /old_locus_tag="pgaptmp_000603"
     CDS             complement(16875..17504)
                     /locus_tag="ID725_RS03015"
                     /old_locus_tag="pgaptmp_000603"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HGV7849277.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17501..19882)
                     /locus_tag="ID725_RS03020"
                     /old_locus_tag="pgaptmp_000604"
     CDS             complement(17501..19882)
                     /locus_tag="ID725_RS03020"
                     /old_locus_tag="pgaptmp_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HGV7849278.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRYSDTVSSLADQWIP
                     LLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHICGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADVLLPETSFMERWNIGETWGTASYLILSEKPIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDRGVSIIPVEITPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
     gene            complement(20046..22358)
                     /gene="pbpC"
                     /locus_tag="ID725_RS03025"
                     /old_locus_tag="pgaptmp_000605"
     CDS             complement(20046..22358)
                     /gene="pbpC"
                     /locus_tag="ID725_RS03025"
                     /old_locus_tag="pgaptmp_000605"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGV7849279.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            complement(22359..27320)
                     /gene="yfhM"
                     /locus_tag="ID725_RS03030"
                     /old_locus_tag="pgaptmp_000606"
     CDS             complement(22359..27320)
                     /gene="yfhM"
                     /locus_tag="ID725_RS03030"
                     /old_locus_tag="pgaptmp_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGV7849280.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            27527..28372
                     /gene="sseA"
                     /locus_tag="ID725_RS03035"
                     /old_locus_tag="pgaptmp_000607"
     CDS             27527..28372
                     /gene="sseA"
                     /locus_tag="ID725_RS03035"
                     /old_locus_tag="pgaptmp_000607"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGV7849281.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            28715..28840
                     /gene="timP"
                     /locus_tag="ID725_RS03040"
                     /old_locus_tag="pgaptmp_000608"
     CDS             28715..28840
                     /gene="timP"
                     /locus_tag="ID725_RS03040"
                     /old_locus_tag="pgaptmp_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGV7849282.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(28872..29648)
                     /gene="sseB"
                     /locus_tag="ID725_RS03045"
                     /old_locus_tag="pgaptmp_000609"
     CDS             complement(28872..29648)
                     /gene="sseB"
                     /locus_tag="ID725_RS03045"
                     /old_locus_tag="pgaptmp_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGV7849283.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(29791..31074)
                     /gene="pepB"
                     /locus_tag="ID725_RS03050"
                     /old_locus_tag="pgaptmp_000610"
     CDS             complement(29791..31074)
                     /gene="pepB"
                     /locus_tag="ID725_RS03050"
                     /old_locus_tag="pgaptmp_000610"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGV7849284.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(31252..31452)
                     /gene="iscX"
                     /locus_tag="ID725_RS03055"
                     /old_locus_tag="pgaptmp_000611"
     CDS             complement(31252..31452)
                     /gene="iscX"
                     /locus_tag="ID725_RS03055"
                     /old_locus_tag="pgaptmp_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGV7849285.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(31464..31799)
                     /gene="fdx"
                     /locus_tag="ID725_RS03060"
                     /old_locus_tag="pgaptmp_000612"
     CDS             complement(31464..31799)
                     /gene="fdx"
                     /locus_tag="ID725_RS03060"
                     /old_locus_tag="pgaptmp_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGV7849286.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(31801..33651)
                     /gene="hscA"
                     /locus_tag="ID725_RS03065"
                     /old_locus_tag="pgaptmp_000613"
     CDS             complement(31801..33651)
                     /gene="hscA"
                     /locus_tag="ID725_RS03065"
                     /old_locus_tag="pgaptmp_000613"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGV7849287.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(33668..34183)
                     /gene="hscB"
                     /locus_tag="ID725_RS03070"
                     /old_locus_tag="pgaptmp_000614"
     CDS             complement(33668..34183)
                     /gene="hscB"
                     /locus_tag="ID725_RS03070"
                     /old_locus_tag="pgaptmp_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGV7849288.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(34279..34602)
                     /gene="iscA"
                     /locus_tag="ID725_RS03075"
                     /old_locus_tag="pgaptmp_000615"
     CDS             complement(34279..34602)
                     /gene="iscA"
                     /locus_tag="ID725_RS03075"
                     /old_locus_tag="pgaptmp_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGV7849289.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(34619..35005)
                     /gene="iscU"
                     /locus_tag="ID725_RS03080"
                     /old_locus_tag="pgaptmp_000616"
     CDS             complement(34619..35005)
                     /gene="iscU"
                     /locus_tag="ID725_RS03080"
                     /old_locus_tag="pgaptmp_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGV7849290.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(35033..36247)
                     /gene="iscS"
                     /locus_tag="ID725_RS03085"
                     /old_locus_tag="pgaptmp_000617"
     CDS             complement(35033..36247)
                     /gene="iscS"
                     /locus_tag="ID725_RS03085"
                     /old_locus_tag="pgaptmp_000617"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGV7849291.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(36359..36847)
                     /gene="iscR"
                     /locus_tag="ID725_RS03090"
                     /old_locus_tag="pgaptmp_000618"
     CDS             complement(36359..36847)
                     /gene="iscR"
                     /locus_tag="ID725_RS03090"
                     /old_locus_tag="pgaptmp_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGV7849292.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(37148..37885)
                     /gene="trmJ"
                     /locus_tag="ID725_RS03095"
                     /old_locus_tag="pgaptmp_000619"
     CDS             complement(37148..37885)
                     /gene="trmJ"
                     /locus_tag="ID725_RS03095"
                     /old_locus_tag="pgaptmp_000619"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGV7849293.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            38004..38807
                     /gene="suhB"
                     /locus_tag="ID725_RS03100"
                     /old_locus_tag="pgaptmp_000620"
     CDS             38004..38807
                     /gene="suhB"
                     /locus_tag="ID725_RS03100"
                     /old_locus_tag="pgaptmp_000620"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGV7849294.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            38952..39806
                     /gene="yfhR"
                     /locus_tag="ID725_RS03105"
                     /old_locus_tag="pgaptmp_000621"
     CDS             38952..39806
                     /gene="yfhR"
                     /locus_tag="ID725_RS03105"
                     /old_locus_tag="pgaptmp_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGV7849295.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            39997..41277
                     /gene="csiE"
                     /locus_tag="ID725_RS03110"
                     /old_locus_tag="pgaptmp_000622"
     CDS             39997..41277
                     /gene="csiE"
                     /locus_tag="ID725_RS03110"
                     /old_locus_tag="pgaptmp_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGV7849296.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(41269..42408)
                     /gene="hcaT"
                     /locus_tag="ID725_RS03115"
                     /old_locus_tag="pgaptmp_000623"
     CDS             complement(41269..42408)
                     /gene="hcaT"
                     /locus_tag="ID725_RS03115"
                     /old_locus_tag="pgaptmp_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGV7849297.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(42568..43458)
                     /gene="hcaR"
                     /locus_tag="ID725_RS03120"
                     /old_locus_tag="pgaptmp_000624"
     CDS             complement(42568..43458)
                     /gene="hcaR"
                     /locus_tag="ID725_RS03120"
                     /old_locus_tag="pgaptmp_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGV7849298.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            43594..44955
                     /gene="hcaE"
                     /locus_tag="ID725_RS03125"
                     /old_locus_tag="pgaptmp_000625"
     CDS             43594..44955
                     /gene="hcaE"
                     /locus_tag="ID725_RS03125"
                     /old_locus_tag="pgaptmp_000625"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGV7849299.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            44952..45470
                     /gene="hcaF"
                     /locus_tag="ID725_RS03130"
                     /old_locus_tag="pgaptmp_000626"
     CDS             44952..45470
                     /gene="hcaF"
                     /locus_tag="ID725_RS03130"
                     /old_locus_tag="pgaptmp_000626"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGV7849300.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            45470..45790
                     /gene="hcaC"
                     /locus_tag="ID725_RS03135"
                     /old_locus_tag="pgaptmp_000627"
     CDS             45470..45790
                     /gene="hcaC"
                     /locus_tag="ID725_RS03135"
                     /old_locus_tag="pgaptmp_000627"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGV7849301.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            45787..46599
                     /gene="hcaB"
                     /locus_tag="ID725_RS03140"
                     /old_locus_tag="pgaptmp_000628"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             45787..46599
                     /gene="hcaB"
                     /locus_tag="ID725_RS03140"
                     /old_locus_tag="pgaptmp_000628"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGV7849302.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            46609..47811
                     /gene="hcaD"
                     /locus_tag="ID725_RS03145"
                     /old_locus_tag="pgaptmp_000629"
     CDS             46609..47811
                     /gene="hcaD"
                     /locus_tag="ID725_RS03145"
                     /old_locus_tag="pgaptmp_000629"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGV7849303.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            47908..48330
                     /gene="yphA"
                     /locus_tag="ID725_RS03150"
                     /old_locus_tag="pgaptmp_000630"
     CDS             47908..48330
                     /gene="yphA"
                     /locus_tag="ID725_RS03150"
                     /old_locus_tag="pgaptmp_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGV7849304.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(48378..49250)
                     /gene="yphB"
                     /locus_tag="ID725_RS03155"
                     /old_locus_tag="pgaptmp_000631"
     CDS             complement(48378..49250)
                     /gene="yphB"
                     /locus_tag="ID725_RS03155"
                     /old_locus_tag="pgaptmp_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGV7849305.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(49262..50356)
                     /gene="yphC"
                     /locus_tag="ID725_RS03160"
                     /old_locus_tag="pgaptmp_000632"
                     /pseudo
     CDS             complement(49262..50356)
                     /gene="yphC"
                     /locus_tag="ID725_RS03160"
                     /old_locus_tag="pgaptmp_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(50389..51387)
                     /gene="yphD"
                     /locus_tag="ID725_RS03165"
                     /old_locus_tag="pgaptmp_000633"
     CDS             complement(50389..51387)
                     /gene="yphD"
                     /locus_tag="ID725_RS03165"
                     /old_locus_tag="pgaptmp_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7849306.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(51412..52923)
                     /gene="yphE"
                     /locus_tag="ID725_RS03170"
                     /old_locus_tag="pgaptmp_000634"
     CDS             complement(51412..52923)
                     /gene="yphE"
                     /locus_tag="ID725_RS03170"
                     /old_locus_tag="pgaptmp_000634"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGV7849307.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(52946..53929)
                     /gene="yphF"
                     /locus_tag="ID725_RS03175"
                     /old_locus_tag="pgaptmp_000635"
     CDS             complement(52946..53929)
                     /gene="yphF"
                     /locus_tag="ID725_RS03175"
                     /old_locus_tag="pgaptmp_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGV7849308.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(54026..57307)
                     /gene="yphG"
                     /locus_tag="ID725_RS03180"
                     /old_locus_tag="pgaptmp_000636"
     CDS             complement(54026..57307)
                     /gene="yphG"
                     /locus_tag="ID725_RS03180"
                     /old_locus_tag="pgaptmp_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGV7849309.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            57425..58618
                     /gene="yphH"
                     /locus_tag="ID725_RS03185"
                     /old_locus_tag="pgaptmp_000637"
     CDS             57425..58618
                     /gene="yphH"
                     /locus_tag="ID725_RS03185"
                     /old_locus_tag="pgaptmp_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGV7849310.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(58681..59934)
                     /gene="glyA"
                     /locus_tag="ID725_RS03190"
                     /old_locus_tag="pgaptmp_000638"
     CDS             complement(58681..59934)
                     /gene="glyA"
                     /locus_tag="ID725_RS03190"
                     /old_locus_tag="pgaptmp_000638"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGV7849311.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            60262..61452
                     /gene="hmpA"
                     /locus_tag="ID725_RS03195"
                     /old_locus_tag="pgaptmp_000639"
     CDS             60262..61452
                     /gene="hmpA"
                     /locus_tag="ID725_RS03195"
                     /old_locus_tag="pgaptmp_000639"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGV7849312.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(61497..61835)
                     /gene="glnB"
                     /locus_tag="ID725_RS03200"
                     /old_locus_tag="pgaptmp_000640"
     CDS             complement(61497..61835)
                     /gene="glnB"
                     /locus_tag="ID725_RS03200"
                     /old_locus_tag="pgaptmp_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGV7849313.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(61896..63230)
                     /gene="glrR"
                     /locus_tag="ID725_RS03205"
                     /old_locus_tag="pgaptmp_000641"
     CDS             complement(61896..63230)
                     /gene="glrR"
                     /locus_tag="ID725_RS03205"
                     /old_locus_tag="pgaptmp_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGV7849314.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(63220..63933)
                     /gene="qseG"
                     /locus_tag="ID725_RS03210"
                     /old_locus_tag="pgaptmp_000642"
     CDS             complement(63220..63933)
                     /gene="qseG"
                     /locus_tag="ID725_RS03210"
                     /old_locus_tag="pgaptmp_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGV7849315.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(64098..65525)
                     /gene="qseE"
                     /locus_tag="ID725_RS03215"
                     /old_locus_tag="pgaptmp_000643"
     CDS             complement(64098..65525)
                     /gene="qseE"
                     /locus_tag="ID725_RS03215"
                     /old_locus_tag="pgaptmp_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGV7849316.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(66101..69988)
                     /gene="purL"
                     /locus_tag="ID725_RS03220"
                     /old_locus_tag="pgaptmp_000644"
                     /gene_synonym="purI"
     CDS             complement(66101..69988)
                     /gene="purL"
                     /locus_tag="ID725_RS03220"
                     /old_locus_tag="pgaptmp_000644"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGV7849317.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            70245..71801
                     /gene="mltF"
                     /locus_tag="ID725_RS03225"
                     /old_locus_tag="pgaptmp_000645"
     CDS             70245..71801
                     /gene="mltF"
                     /locus_tag="ID725_RS03225"
                     /old_locus_tag="pgaptmp_000645"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGV7849318.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(71798..72301)
                     /gene="tadA"
                     /locus_tag="ID725_RS03230"
                     /old_locus_tag="pgaptmp_000646"
     CDS             complement(71798..72301)
                     /gene="tadA"
                     /locus_tag="ID725_RS03230"
                     /old_locus_tag="pgaptmp_000646"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGV7849319.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(72359..72994)
                     /gene="yfhb"
                     /locus_tag="ID725_RS03235"
                     /old_locus_tag="pgaptmp_000647"
     CDS             complement(72359..72994)
                     /gene="yfhb"
                     /locus_tag="ID725_RS03235"
                     /old_locus_tag="pgaptmp_000647"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGV7849320.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            73203..74051
                     /gene="yfhH"
                     /locus_tag="ID725_RS03240"
                     /old_locus_tag="pgaptmp_000648"
     CDS             73203..74051
                     /gene="yfhH"
                     /locus_tag="ID725_RS03240"
                     /old_locus_tag="pgaptmp_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGV7849321.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            74107..74367
                     /gene="yfhL"
                     /locus_tag="ID725_RS03245"
                     /old_locus_tag="pgaptmp_000649"
     CDS             74107..74367
                     /gene="yfhL"
                     /locus_tag="ID725_RS03245"
                     /old_locus_tag="pgaptmp_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGV7849322.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(74561..74641)
                     /gene="shoB"
                     /locus_tag="ID725_RS03250"
                     /old_locus_tag="pgaptmp_000650"
     CDS             complement(74561..74641)
                     /gene="shoB"
                     /locus_tag="ID725_RS03250"
                     /old_locus_tag="pgaptmp_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGV7849323.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(75063..75443)
                     /gene="acpS"
                     /locus_tag="ID725_RS03255"
                     /old_locus_tag="pgaptmp_000651"
     CDS             complement(75063..75443)
                     /gene="acpS"
                     /locus_tag="ID725_RS03255"
                     /old_locus_tag="pgaptmp_000651"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGV7849324.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(75443..76174)
                     /gene="pdxJ"
                     /locus_tag="ID725_RS03260"
                     /old_locus_tag="pgaptmp_000652"
     CDS             complement(75443..76174)
                     /gene="pdxJ"
                     /locus_tag="ID725_RS03260"
                     /old_locus_tag="pgaptmp_000652"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGV7849325.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(76186..76914)
                     /gene="recO"
                     /locus_tag="ID725_RS03265"
                     /old_locus_tag="pgaptmp_000653"
     CDS             complement(76186..76914)
                     /gene="recO"
                     /locus_tag="ID725_RS03265"
                     /old_locus_tag="pgaptmp_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGV7849326.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(76926..77831)
                     /gene="era"
                     /locus_tag="ID725_RS03270"
                     /old_locus_tag="pgaptmp_000654"
     CDS             complement(76926..77831)
                     /gene="era"
                     /locus_tag="ID725_RS03270"
                     /old_locus_tag="pgaptmp_000654"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGV7849327.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(77828..78508)
                     /gene="rnc"
                     /locus_tag="ID725_RS03275"
                     /old_locus_tag="pgaptmp_000655"
     CDS             complement(77828..78508)
                     /gene="rnc"
                     /locus_tag="ID725_RS03275"
                     /old_locus_tag="pgaptmp_000655"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGV7849328.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(78781..79755)
                     /gene="lepB"
                     /locus_tag="ID725_RS03280"
                     /old_locus_tag="pgaptmp_000656"
     CDS             complement(78781..79755)
                     /gene="lepB"
                     /locus_tag="ID725_RS03280"
                     /old_locus_tag="pgaptmp_000656"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGV7849329.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(79771..81570)
                     /gene="lepA"
                     /locus_tag="ID725_RS03285"
                     /old_locus_tag="pgaptmp_000657"
     CDS             complement(79771..81570)
                     /gene="lepA"
                     /locus_tag="ID725_RS03285"
                     /old_locus_tag="pgaptmp_000657"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGV7849330.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(81768..82247)
                     /gene="rseC"
                     /locus_tag="ID725_RS03290"
                     /old_locus_tag="pgaptmp_000658"
     CDS             complement(81768..82247)
                     /gene="rseC"
                     /locus_tag="ID725_RS03290"
                     /old_locus_tag="pgaptmp_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGV7849331.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(82244..83200)
                     /gene="rseB"
                     /locus_tag="ID725_RS03295"
                     /old_locus_tag="pgaptmp_000659"
     CDS             complement(82244..83200)
                     /gene="rseB"
                     /locus_tag="ID725_RS03295"
                     /old_locus_tag="pgaptmp_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGV7849332.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(83200..83838)
                     /gene="rseA"
                     /locus_tag="ID725_RS03300"
                     /old_locus_tag="pgaptmp_000660"
     CDS             complement(83200..83838)
                     /gene="rseA"
                     /locus_tag="ID725_RS03300"
                     /old_locus_tag="pgaptmp_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGV7849333.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(83871..84446)
                     /gene="rpoE"
                     /locus_tag="ID725_RS03305"
                     /old_locus_tag="pgaptmp_000661"
     CDS             complement(83871..84446)
                     /gene="rpoE"
                     /locus_tag="ID725_RS03305"
                     /old_locus_tag="pgaptmp_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGV7849334.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(84443..84598)
                     /gene="rseD"
                     /locus_tag="ID725_RS03310"
                     /old_locus_tag="pgaptmp_000662"
     CDS             complement(84443..84598)
                     /gene="rseD"
                     /locus_tag="ID725_RS03310"
                     /old_locus_tag="pgaptmp_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGV7849335.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            84854..86476
                     /gene="nadB"
                     /locus_tag="ID725_RS03315"
                     /old_locus_tag="pgaptmp_000663"
     CDS             84854..86476
                     /gene="nadB"
                     /locus_tag="ID725_RS03315"
                     /old_locus_tag="pgaptmp_000663"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGV7849336.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(86461..87198)
                     /gene="trmN"
                     /locus_tag="ID725_RS03320"
                     /old_locus_tag="pgaptmp_000664"
     CDS             complement(86461..87198)
                     /gene="trmN"
                     /locus_tag="ID725_RS03320"
                     /old_locus_tag="pgaptmp_000664"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGV7849337.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKAGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            87330..88664
                     /gene="srmB"
                     /locus_tag="ID725_RS03325"
                     /old_locus_tag="pgaptmp_000665"
     CDS             87330..88664
                     /gene="srmB"
                     /locus_tag="ID725_RS03325"
                     /old_locus_tag="pgaptmp_000665"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGV7849338.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(88697..89578)
                     /gene="yfiE"
                     /locus_tag="ID725_RS03330"
                     /old_locus_tag="pgaptmp_000666"
     CDS             complement(88697..89578)
                     /gene="yfiE"
                     /locus_tag="ID725_RS03330"
                     /old_locus_tag="pgaptmp_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7849339.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            89681..90268
                     /gene="eamB"
                     /locus_tag="ID725_RS03335"
                     /old_locus_tag="pgaptmp_000667"
     CDS             89681..90268
                     /gene="eamB"
                     /locus_tag="ID725_RS03335"
                     /old_locus_tag="pgaptmp_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGV7849340.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(90324..90707)
                     /gene="grcA"
                     /locus_tag="ID725_RS03340"
                     /old_locus_tag="pgaptmp_000668"
     CDS             complement(90324..90707)
                     /gene="grcA"
                     /locus_tag="ID725_RS03340"
                     /old_locus_tag="pgaptmp_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGV7849341.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            91012..91701
                     /gene="ung"
                     /locus_tag="ID725_RS03345"
                     /old_locus_tag="pgaptmp_000669"
     CDS             91012..91701
                     /gene="ung"
                     /locus_tag="ID725_RS03345"
                     /old_locus_tag="pgaptmp_000669"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGV7849342.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(91749..92786)
                     /gene="yfiF"
                     /locus_tag="ID725_RS03350"
                     /old_locus_tag="pgaptmp_000670"
     CDS             complement(91749..92786)
                     /gene="yfiF"
                     /locus_tag="ID725_RS03350"
                     /old_locus_tag="pgaptmp_000670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGV7849343.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            92993..93412
                     /gene="trxC"
                     /locus_tag="ID725_RS03355"
                     /old_locus_tag="pgaptmp_000671"
     CDS             92993..93412
                     /gene="trxC"
                     /locus_tag="ID725_RS03355"
                     /old_locus_tag="pgaptmp_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGV7849344.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            93481..94179
                     /gene="tapT"
                     /locus_tag="ID725_RS03360"
                     /old_locus_tag="pgaptmp_000672"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             93481..94179
                     /gene="tapT"
                     /locus_tag="ID725_RS03360"
                     /old_locus_tag="pgaptmp_000672"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGV7849345.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            94211..96871
                     /gene="pat"
                     /locus_tag="ID725_RS03365"
                     /old_locus_tag="pgaptmp_000673"
     CDS             94211..96871
                     /gene="pat"
                     /locus_tag="ID725_RS03365"
                     /old_locus_tag="pgaptmp_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGV7849346.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            96985..98340
                     /gene="pssA"
                     /locus_tag="ID725_RS03370"
                     /old_locus_tag="pgaptmp_000674"
     CDS             96985..98340
                     /gene="pssA"
                     /locus_tag="ID725_RS03370"
                     /old_locus_tag="pgaptmp_000674"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGV7849347.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLLDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            98386..98709
                     /gene="yfiM"
                     /locus_tag="ID725_RS03375"
                     /old_locus_tag="pgaptmp_000675"
     CDS             98386..98709
                     /gene="yfiM"
                     /locus_tag="ID725_RS03375"
                     /old_locus_tag="pgaptmp_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGV7849348.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(98706..100004)
                     /gene="kgtP"
                     /locus_tag="ID725_RS03380"
                     /old_locus_tag="pgaptmp_000676"
     CDS             complement(98706..100004)
                     /gene="kgtP"
                     /locus_tag="ID725_RS03380"
                     /old_locus_tag="pgaptmp_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGV7849349.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(100121..>100313)
                     /locus_tag="ID725_RS03385"
                     /old_locus_tag="pgaptmp_000677"
     CDS             complement(100121..>100313)
                     /locus_tag="ID725_RS03385"
                     /old_locus_tag="pgaptmp_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849350.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
BASE COUNT        24076 a        27298 c        25073 g        23866 t
ORIGIN      
        1 aaatttaaaa aatgtcttct gcctgtggca atgttagcgt cattcactct ggcaggatgc
       61 cagtcaaatg ctgacgatca tgccgccgat gtttatcaaa ccgatcaact gaataccaaa
      121 caagaaacta aaaccgttaa tattatttcc attcttcccg caaaagttgc cgtagacaac
      181 tcccaaaata aacggaacgc acaagccttc ggcgcgctta ttggcgcagt cgctggcggt
      241 gttatcggcc acaacgtcgg gtctggcagc aattccggaa cgacggcagg tgcagttggc
      301 ggcggagctg taggcgcggc agcgggttct atggtgaatg ataaaacctt agtggaaggt
      361 gtttctttaa cgtataagga aggcaccaaa gtgtatacct ctactcaggt gggtaaagag
      421 tgccagttta cgacaggttt agccgttgtt attaccacga cgtataacga aacgcgtatt
      481 cagccaaata ccaaatgtcc tgaaaagagc taataatcag gaggagtcat gaagaaagtt
      541 tttctttgcg ccatcttagc ctccttaagc tatccggcta tcgcctcatc attgcaggat
      601 caactctctg ctgtcgcaga agcggaacag caaggtaaaa atgaagagca aaggcagcat
      661 gacgaatggg tcgcggagcg caacagggaa atccagcaag agaagcaacg tcgcgcaaat
      721 gcccaggctg ccgctaacaa aagagcggca acggcagcgg caaataagaa agctcgtcag
      781 gataaactgg acgccgaagc cactgcggac aaaaaacgcg atcaaagtta tgaagatgag
      841 ctacgcagct tagagattca gaaacaaaaa ctggcgctgg caaaagaaga agcccgcgtt
      901 aagcgagaaa acgaatttat cgatcaggag ctgaagcaca aagccgcgca aactgatgtc
      961 gtgcaatctg aagctgacgc caacagaaat atgactgaag gcggtcgcga tctgatgaaa
     1021 agcgtgggca aagcagaaga gaataaatcg gacagctggt ttaattaatc gatgttagta
     1081 acttcaagcc tataattctt gaagataaaa aaccctctgt agtaacagag ggttttgttc
     1141 attcatagtg cagggtcaaa tcattcccac tcaatggtag ctggtggctt gccgctgatg
     1201 tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga aacgcgaccg
     1261 aggaaatcgt acggcagatg cgcccagtgt gcggtcataa agtcgatggt ttcgacagca
     1321 cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac ggaacgtact
     1381 ggcaggaaga cggtgaacgc ctggctgact ttgtcgtaca ggtccgcttt acgcagttct
     1441 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
     1501 agaacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacagcat gtcgtacggc
     1561 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1621 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
     1681 tgtgctttac cggttgcaga cgccgcagat tcgataacgt cagggtagat ggtgccctgc
     1741 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc aacgaaaacg
     1801 cgaccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
     1861 cgatcttctg ccggtacgtg aacaatgtta agaccaaagt gatcgccaaa catatccaga
     1921 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcaggttt
     1981 ttaccgatag cgcggtgcag cagcattgcg gttacggagg aatccacacc accagagagg
     2041 ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gagctacagc atcgtcgata
     2101 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     2161 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     2221 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgacgaag
     2281 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
     2341 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
     2401 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     2461 gccatggtct gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     2521 tgcggcgcac gcgggctgtt ttcttcagta gtactttccg ggccgccgga aagaataatg
     2581 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccatgccca cagttcgcag
     2641 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
     2701 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2761 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2821 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2881 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     2941 tcaaacccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     3001 ctttataagc tacgcgacct tcgatacctt ccggcaccag tttgtcggca gcgttatcgc
     3061 tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc ataccacggt
     3121 aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacccg
     3181 ccagcatgga acctaccatc accgcgcttg cgccagcggc gatagctttg gcgatgtcgc
     3241 cggagaagcg aataccacca tctgcgataa ccggaatacc ggtgccttcc agggcttcaa
     3301 ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata cgagtcgtac
     3361 agatagaacc agggccgata ccgactttaa ccgcgctgca accagcttct gccagagcgc
     3421 gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga tatttagcac
     3481 gggtttcacg gatacgctgc aaaacgcctt ctgagtgacc gtgggaggag tcgatcagca
     3541 gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
     3601 caactgcagc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
     3661 ccgctttctg gaaatctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
     3721 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
     3781 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
     3841 ggtcggtaac aaaacgcacg tcacgaccgg tgataatccc caccagttcg ttttcttcgg
     3901 taacgaccgg atagcccgca aaaccgttac gctcggtcag ttctttcact tcgcgcagcg
     3961 tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt tttttcacac
     4021 ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
     4081 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     4141 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
     4201 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     4261 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     4321 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
     4381 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     4441 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
     4501 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt cacacaaccg
     4561 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
     4621 ttccgcaaca gcaaccgccg ggtgaccttc cgcccacagc atgggcaaca agttttagtt
     4681 cgcgccaata ttacgctcta cgagccgcgc ggcgactacc agataatcgt tgagagtatg
     4741 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
     4801 gctgaaggat tgttcgatct gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
     4861 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4921 cgcgatcctt ctctgccggt gatcatctac cccaccgccg ttcagggcga tgacgcgccg
     4981 gggcaaattg ttcgcgccat tgaactggcg aatcagtgca atgagtgcga cgtgttgatc
     5041 gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
     5101 cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga gacggatgtg
     5161 accattgccg attttgttgc cgatctgcgt gcaccaacac cgtcggctgc cgccgaagtg
     5221 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cgacccgtca acggctggag
     5281 atggcgatgg attattatct cgccaaccgc acacgtcgct ttacgcaaat tcatcaccga
     5341 ttacagcaac agcatccgca gctccggctg gcacgccagc aaaccatgct tgaacgcctg
     5401 caaaaacgga tgagctttgc gctggaaaat caacttaagc gtgccgggca acagcagcag
     5461 cgattaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cgcgcaaacg
     5521 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgcacagct tagcgccacg
     5581 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
     5641 gcgcgtggat acagcgttac tactgctact gacggcaagg tactgaaaaa agtgaagcag
     5701 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctgggtaga aagtgaagta
     5761 aaaaacatcc agccggtaaa aaaatcgcgt aaaaaagtgc attaagccag cacgaattca
     5821 acgcgttttt tcgatatcaa gccatgcccg tgctgacaga agtaatctac cgcaccgcag
     5881 gcttttaaca cctgcaacgg ttgatggcag tccgggcaaa gagctttcat ttcaataact
     5941 ttcccgcagg atgggcaata cgcatggccg ttatcctgat caagcacatg ctgacattgt
     6001 ggacaatgca gttccatatg aattcctccg gtagccaccc ctctacattc atagagggaa
     6061 tggcagataa aatacttacg gataattatt tatttttctt gatgtgcttc atcagacgct
     6121 tacgtttacg catctgggtt ggcgtcaggg tgttacgctt gttcgcatac gggttttccc
     6181 cttctttgaa ctgaatacga atcggcgatc ccattacgtc cagcgatttg cggaagtagt
     6241 tcatcaagta acgcttgtag gaatcaggca ggtctttcac ctgattaccg tgaatcacca
     6301 caatcggcgg attataacca ccggcgtgtg catatttcag cttcacacga cgaccgcgta
     6361 ccagcggcgg ttggtgatct tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc
     6421 ccacacgacg ggtggagctg tcatacgctt cacgtactga ttcaaacaag ttaccaacac
     6481 cactgccgtg caaggcagag ataaagtgca cacgagcaaa atcgataaag cccagacgga
     6541 agtccagcgt ttctttcacc tgctctttca cttcctgact caggccatcc cacttattca
     6601 ccacaatgac aagtgagcgc ccactattga gaataaagcc cagcagcgag agatcctgat
     6661 cggaaatacc ttcgcgcgca tcaatcacta acatcaccac gttggcgtct tcaatggcct
     6721 gcaacgtttt gattacggag aatttctcta cagcatcggt gattttgccg cgtttacgta
     6781 cgccagcggt gtcaatgagc acatactcac gtccatcgcg ttccattgga atgtagatac
     6841 tgtcacgcgt cgtaccaggc atgtcataaa caacaacgcg ctcttcacca agaatacggt
     6901 tagtgagtgt agacttacct acgttcggac gacccacaat cgccagtttg atcggcagac
     6961 tttgcgggtc gaagtcgtct tcctcttctt cttcgccgtt ctcttccgct tcaaattgcg
     7021 cccagtattc agcgtcttcg tcgacttcct cttgcggtgc gagatcttcc atccacggca
     7081 gtagcacatg ctccagcaga cttaatacgc cacgaccgtg agacgcggcg atcgggtaga
     7141 tttcacctaa accaagcgag tagaaatcaa ccactgcctg atcgggatcc agaccgtcag
     7201 ttttgtttgc caccaggaag gtcggttttt cacgggagcg cagatgtttg gcaatcgctt
     7261 catctgccgg catcaggccc gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt
     7321 caatcgccag cagcgactgt tccgccatgc gggtttctac accgtcttct gtgccatcaa
     7381 tcccgccggt atcaatacag ataaactcac ggccttcaat ttccgcacga ccgtacttac
     7441 ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt
     7501 taaataacgt ggattttcct acgttagggc gcccgacaag cgcgaccaca ggtaccatgt
     7561 ttaaagcctc atttttataa atcatcagac aacgcacgct atattcgcgt cgttgttaaa
     7621 aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt gaacgacaga gacgattaac
     7681 gtgtaataga gtacacggtt ccgtcttttg cctggatcag cagtttgccg tcagcggcaa
     7741 ccggttcagt ctggaaaccg gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt
     7801 cgacgttaat ccagtgcaaa taaccttcac tgtcaccgac caccaggttg ccattataca
     7861 gcaccggaga agtcagcagg cgatgcagca gatcgctttg tgtccacagc gtaacgccgc
     7921 catcaatggt caacgccatc acccggtcat tttgatcgac cagatagatg cgattgccgt
     7981 cgacgatgaa atcattcacc gaacccagtt cgcgtttcca cataatctga ccactgcgca
     8041 gatcaagcgc cgtcaggtta ccattatagg ccagcgcgaa aacaacgccg ttaacaacgg
     8101 cgggagtcgt gtcaacatcg ctcagacggt caatttcggt agaaccggtc gcttgggaaa
     8161 tacgctgctg ccaaatcatc tggccctgtt ccatcagcac tgcgctgacg cgaccattat
     8221 cgccccccac gacggccgca ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga
     8281 gcgaaggcat atcgaggtta actgtccatt tgacagcgcc gtcagcttcg ttcagcgctt
     8341 gtaactgacc gttactggtg tggattaaca ccagaccgtc gctgaccacc gggcgcgaaa
     8401 gtgcttcacc cgcgacttta gtttgccatg ccacagtacc atcgctggta ttcagcgcgt
     8461 aaacctgcgc cttttcgctg ccaatgtaga catgtccacc agacacggtc acaccgccag
     8521 aaagtaatgc aggctcttta gagaaccagc catctttctc ggccaggctg acagaccaga
     8581 tttctttgcc atcatccgca ttcagcgctt ttactaaacc agcgcggtcc gctgcataga
     8641 caacgttgtc cgccagtgcc ggatgaagat tggaatagaa gttgccaatg ccgctaccaa
     8701 cggaagtgct ccacgccgtg gtcggcgtaa actggttttc aacggttggc aatggggaca
     8761 tctttaccac atcttcttcg ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa
     8821 gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc cctctcagat ggacaaatta
     8881 ttaattttca tctgcatcat ttcgctcagt gccggagtaa catcgctttt cacgcctgct
     8941 tcccatgcac tacgcgcacc ttgcttatca cctttgctca gcaatgcttc accacgcagg
     9001 tcggcaacaa tggcagccca cccttcacct ttgatggtat caagggtttt cagcgcggca
     9061 tcagcctgct tgagctgtac ctgaacgcga gcaagacgca gatttatcac ggctttgaga
     9121 ttttcatcgc tcgtgtctgc cagcccctgt tgtaactggg cggcagcttt ctctagttca
     9181 tttttgtcaa caaattgctg cgcaagttcc aaagaagcca gcgcaccata agtattttta
     9241 ttttcagcag caaatttttc cgccgccggg atgctatccg gtttgccttc gctcactgcg
     9301 gtaaccgcat tttgataggc aagagaagcg gagcgtgcag aatcaacctg atggctgttc
     9361 cagtagcgcc agccaatcag tgcgccaacg cccaaaatca ccccaacagc cagtgctttg
     9421 ccattttcag caaaaaagcg tttaatcgct tctacctggt cgttttcgtt ctcgtaaatt
     9481 tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg caaatgcgcg gctacgctat
     9541 cctgcgcaac tgccgtttgc tcaccagagc gcaaatcctt cactactgct gtgccgttag
     9601 ccacttcaga ctcacccagc accacagcaa cgcgggcacc ccatttatca gcacgggcaa
     9661 actgtttctt aaagttgccg ccgccgtggt tggtcatcaa tttcacgccc ggtaattcat
     9721 cacgcagacg ctcagctaat gccatagccg cagattgtgt atcagcacct gaagccacca
     9781 ggtatatatc gacaacagga tcggctttaa attccggatt aacggcctgt actaacaata
     9841 caagacgttc gaggcccatc gcaaaaccga cagccggtgt tgcacggccg cccagttgtt
     9901 ccacaagacc gtcataacga ccgcctgcac acacggtgcc ctgggagccg agactgttag
     9961 tcacccactc gaaaacggta cggttgtagt aatccagacc acgcaccaga cgctggttta
    10021 cggtgtaagc gatccccgcg ctctcaagca gtttgcacag accggcaaaa tgctcacgag
    10081 attcctcgtc cagataatca cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg
    10141 gattttttga atccagcacg cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt
    10201 ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg
    10261 cttccagcga accgatagaa ttcagctcaa gagttacgtg ttcggaaata cccagcgcgc
    10321 gccaccagcg ggcggtgagc ataatcagtt cagcgtcgat atccggacct tgcagaccga
    10381 aaacttcgca gcccaactga tggaactgac gataacgccc tttctgcgga cgctcgtgac
    10441 ggaacatcgg cccgatatac cacagacgct gttcctgatt gtacagaaga ccatgctcga
    10501 tgccggcgcg tacacagccc gccgtccctt cagggcgcag agtcaggctg tcgccattgc
    10561 gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt gacttcacca atcgcacgtt
    10621 tgaatagcgg ggtctgctct acaatcggca agcggatttc actgtaaccg tagctgccga
    10681 gcacgttttt cagtgtgcct tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat
    10741 cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt tattctcttt ctgaatataa
    10801 aaatgaaccc tcaacgcttc cctcaatgtt tcgggagcca tgcgggttca atcatacacg
    10861 ggaagcgagg cgcttcccat cacgttatta tttttcaacc tgctgaacgt caattcgacg
    10921 cgcttcgtcc agctgactgg ctttcgcacg aatgcgtgcc tccagctggt cgatcatatc
    10981 gttgttgtcc agacggtctt tgcgcacgcc atcttcatag agaccgcttt tcttgttgcc
    11041 gccggtgacg ccgagtgtag aaaccagcgc ctcacccggg ccattcacca cgcagccgat
    11101 aatcgaaacg tccatcggag tgatgatatc ttccaggcgt tgctccagcg cgttaactgt
    11161 accgataaca tcaaattcct gacgcgaaca ggtcggacag gcgatgaagt tgatacctcg
    11221 cgaacggata cgaagcgatt tcaaaatatc gaaaccgact ttgatctctt cgaccggatc
    11281 ggccgccagt gatacgcgca gcgtgtcgcc gatgccttca gacagcagca gacctaaacc
    11341 aatggcggat tttactgccc cgctgcgcgc gccacccgct tcggtgatcc ccagatgcag
    11401 cggctgatcg atttgttttg ccagcaaacg ataagactca acagcgagga agacgtcaga
    11461 cgctttcacg ctgactttga actgatcgaa gttcaggcga tcgagatgat caacatggcg
    11521 catggcggat tccagcaacg cctgcggcgt cggttcgcca tacttttctt gcagatcttt
    11581 ttccagcgat ccggcgttaa cgccaatacg gatcggaatg tttttatcgc gcgcacagtc
    11641 aaccaccatg cgaatacgct cttcattacc gatattgcca gggttaatac gcagacaatc
    11701 gacgccgtat tccgctactt tcagcgcaat gcgatagtcg aagtggatgt cagccaccag
    11761 cggcacgtta acctgctgtt tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac
    11821 ggagacacgg acgatatcag cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc
    11881 ttcgacatct gtcgtacgcg tattggtcat ggactgtacg gcgatgggag caccatcgcc
    11941 aatcggcaca ttcccaacgt aaatacgtgt tgattttcta cgttgaattg gagcctggtt
    12001 atgcatgaaa aatctcccgc gttacccgtc tgttactgcg ccggtgattg ttcggcattg
    12061 agggtcagac gcgcaacctg gttagttctg ataaaacgac tcagatcgac aggtttccct
    12121 tgatactgga tctgtactgc ggctggcgca ccaattttca gtttgtacgg tgcctggccg
    12181 gttaagttca aattaccgtc tttacgctgc ataccgctaa acaatttttt accggtagca
    12241 tcagtgacct ccagccagca atcggcagta aagttcatca ccagcgcatt cggatcagcc
    12301 accggcgtgg tcacgccagc ctgatcggtt ggcaacggcg cagcaccatc tggcgttgtt
    12361 gctgccgttg gtgccggggt cgcggcggta tcaacatttg cctgcgaagg cgaaacaacc
    12421 gcattctgtt gcggatcaac agctggtgct ggcgcagtta cgacaggtgt ttgcgtgttg
    12481 gttgcggtag tatccacaga cgctggcggc gtggacgttg tagccgggtc tgtagttgtc
    12541 gacgtattta acggaacact ctgcccctgc tcgctattac tgctcagttc cgccgaagat
    12601 tgatcggcca tagtggtgat ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca
    12661 ccgctcaggc cgataaccac aaacaacacc agccaggtga aggtcatcag ccagccgtcg
    12721 cgttttttgc ggcgtttacc gagggaaaaa ctctgcatcg gcgcaacttt tgcagcccgt
    12781 agtggagcct gcttttccag ccctggcagc agttcttctt ctggaatatg taccagacgc
    12841 gcataagagc ggatatatcc gcgcaggaat gttgaagcaa gatcggcggg tgccttatct
    12901 tcttcaatgt cgcgtaccgt ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga
    12961 ctaagtccta gttgttcgcg agcattacgc aggcgagcgc cggtagtaag tgcttcattt
    13021 tggtcgtgcg tggcttcagt attcattcgc tgcaggtacg tttaaatgag aattgagatg
    13081 ccggtgaacc atcatgctca cccacaccgc gaaacatccg ttaagttaac cgttatcata
    13141 cagtataaga ccgtctgtgt gctcgtgaca aagcccacat tcactaaggc taacttactg
    13201 ttgcatcgtt acatactgcc ttaaagtcag caaaaacgca ccgttaatat tgaccagaca
    13261 attgcaactt aatacttcat tcataaaaca ttacgccacg gtacataaag taaccgtggc
    13321 gtaatggcta tcagaccgct ttaatgtcga tggcttcacc ctgcatccgt ttacgcaggg
    13381 tacgtttcgt acggtcgata acatcgcccg ccaactgacc acaggcagca tcgatatcat
    13441 caccacgcgt tttacgcaca atagtggtga aaccgtagct catcagcact tttgagaaac
    13501 ggtcgatacg gctgttcgag ctgcgtccat acggcgcagc cgggaacggg ttccacggga
    13561 tcaggttgat cttacacggc gtatctttca gcagttccgc cagttggtgc gcgtgttcag
    13621 tgccgtcgtt aacgtggtca agcatcacgt attcaatagt gactcggccc tgattggcgt
    13681 tggatttctc cagataacgg cgcaccgcag caaggaacgt ttcgatattg tactttttgt
    13741 tgatcggcac aatttcgtca cgaatttcgt cgttcggcgc gtgcagggaa attgccagcg
    13801 caacatcgat catatcgccc agtttatcca gcgctggaac tacaccggaa gtggaaagcg
    13861 tcacgcgacg tttagacaga ccaaaaccga aatcatcaag catgatttcc atcgccggaa
    13921 cgacgttgtt caggttgagc agcggctcgc ccatgcccat catcactacg ttagtgattg
    13981 ggcgctgacc ggtgactttt gctgcgccga cgattttcgc cgcacgccac acctggccaa
    14041 taatttccga tacccgcagg ttgcggttaa agccctgctg ggcggtggaa cagaatttac
    14101 actccagcgc acaccccacc tgcgaagaga cgcagagcgt agcgcggtcg tcttccggga
    14161 tgtacaccgt ttcgacacgc tggtcaccca cggcaatcgc ccatttaatg gtgccgtcag
    14221 atgaacgctg ttcttcaacc acttccggtg cgcggatctc cgccacctct ttcagtttgc
    14281 cgcgcaacac tttgttgatg tcggtcatct catcaaaatt gtcgcagcaa tagtgataca
    14341 tccacttcat cacctgatcg gcgcggaagg gtttttcacc taaatcttta aaaaactccc
    14401 gcatctgctg acgattgaga tccagcaggt tgatttttcc atctttggtg gtgacgtttt
    14461 caggtgtgac taattgttca gacatatgct attccggcct cgttattaca cgttatggcc
    14521 cctggagggt tgaaaaaaga aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat
    14581 tgtacaaatt ctggcgcacg gatgccacgt ttgcacgcgg catttacgaa attattaacg
    14641 ggtgcgcggg cacacttcgc cttcgccgaa gaaataagcg atttcgcgag cggcagattc
    14701 gacggaatca gaaccgtggg taccgttttc ggtcaggctg tcagcgtaat cagcgcgcag
    14761 agtaccggcc agtgcgtttg ccggattggt cgcgcccagc agatcgcggt gacgctgaac
    14821 ggcgttttca ccttccagca cggaaaccac gatcgggcca gaggtcatga attcaaccag
    14881 accatcaaag aacggttttc catcgtgtcc agcataaaag ccacgtgcct gttcaacggt
    14941 caggtgcagc attttggtgc cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat
    15001 attaccaatg acgttttttg ctaccgcgtt cggtttgata atggaaaaag tacgttcaat
    15061 agccattttt acctctgtaa attgttctgt tgttgtctgt accagcgtac gagatggcgc
    15121 ggattataat gagcaacaga gccgttgact attgatgaag gtaacatttt tttaaaataa
    15181 aatgagtttt agcaacaact cgacttttat ggtgttctca tcccataacg atgacgttgg
    15241 caataaagac aattttgcgc attttgctga aaagtaagaa aggatttttg acaggtttta
    15301 cagactttgc gattagcatg aagattcaca atttttgccg gtagaatcgt caattcaacg
    15361 gtcattgcct gatgaaaaca aacagcaata caattttggc atccgttaca caaggcatca
    15421 tttatttgta aatggccttc gttaagcgaa aagacctgtt gctcgcaggc ccgccagcat
    15481 gcaccgcaaa gctgacactt ttgaggatcc atcatcgggc gagcatcact aatctggggc
    15541 cataaataat gtaaccgacg taatcctggt tgtatttgtg tcatctgaac atccgcacgc
    15601 tgcacatgga acaaacgacg ccgggaaaca ttcacctcac tggtatgtgg agataccaca
    15661 gaccagcctt cctcgccata ttgccgtaag cattcgttta acagcaataa tgcgcgctcc
    15721 catgccttcg cctgactcag atcaatcgcg atcgctgtga tcccctgctc tttatgccat
    15781 agcaaaagtt cttccagcgt cggcgcaata gtggtgaaag gtgcgaccag ggtgtgattg
    15841 caaaaataac gtaaagggga attaacgcca cgaatagcct cttgcggaca aataaactgg
    15901 caaaccgcac aatcaataca ttgttccgcg tcgaccgcta tgccctcttc tgtcagcgtt
    15961 agcgcctgtg ccgggcaagc ctcgacacag gcatgacatg ttgcaaaacg aaagcgttgg
    16021 cgcgcgcatt ccgcgccaat cgttatgtta tgacttaaca ttagccgata ctaaaaaaga
    16081 caaaacgcag cataacttca ctccccacta gcattacgct accgaataac gccgccggga
    16141 tattcttttt acaaccaccg acagcaaaga acatcatccc aatgacggaa attccccagc
    16201 ttagtgtcag tatgggtttt aactgtccca gcatcgctaa tggcgcatgt ggcaatgtgg
    16261 ctatctgcat cgcatttgcc gttaaatccc ctatccacaa cggctgaacc aacaaacgca
    16321 gcagaaccag gagagcgatc accaacacgc atccattacg cagggcatca atctgcggca
    16381 aaattccgct aatacgaaac actgctacca gcgctgaacc gagaattccc actgtaccta
    16441 agaacataat cagcgtgttg taatgctgcc aggtgataat cgacgtatgg atgtaaactt
    16501 gcgccatgca gaaaacatca acaatcccga ccactgccgc tagcataagc aggagttgcc
    16561 agccgccttt gcgtaacatg accagtaatg tggataaacc caatagcgcc agatacagcg
    16621 caccaaagat aatttcccga ctcagccatg aactggaaaa gtggctcagc gcatgcatcg
    16681 cattaagtgg ataacccagg tgtaaggtag aaatcagtaa ccctacggca cctgccaaaa
    16741 aagccaacag taatggcggc cgcataaccg ggcgggcgct ggaggtgatt aaaatatgac
    16801 gatgaagaaa cgccagccac aacgtcatac cgaccgatgc ctgtaacaac agcgtaaaag
    16861 ccattaacgg taattcatgc atgatgtgac gcctcctttt ccgcgccctg atgcggttta
    16921 atcaccagat ttggctgcgt aatcgacgaa tcaggtaaac ctttcacatc actgacatcg
    16981 ccatactttt cacgcaactc atcaataggg ccgaacttaa tcgcccccaa cgggcacgtt
    17041 gccacgcaaa tgggttgttc tcctttagct tgcagatcaa tacagaaatc gcatttcgac
    17101 atctgcccgg tctgctcatt catttgtggt gcgccgtaag gacaggacca ggcgcagtaa
    17161 ccgcaaccga cgcacttatc agtattcacg cgcacaatac cgtctccggg acgcttatgc
    17221 atcgccatcg tcgggcaatt tttggtacag atcggatccg cgcaatggtt acaggaaaca
    17281 gaaagggtat aagcaaagac attatgactc acaccgccct ctccagtggc gatgaagcca
    17341 cctccgttca cttcatacac gcggcgaaaa cgtcgcccaa cctcaaggtt gtttttgtct
    17401 ttacaggcca cctgacacgc tttacagccc gaacaacgcg aagagtcaat aaagaaccct
    17461 aattgtttgt cgctgacaga tgcaaattct ttaaattggc tcatgctttc tcaacctcca
    17521 ccagcattgt ttgatgcgaa ttccctttcg ccagcgccgt aatgcgggtc gaactcagta
    17581 cattggcaca gcctccgcga tcgatacctt gtgcatccgg ttgccaccat gcccccgcct
    17641 gcatagcaac tacgccaggg atgatacggg gggttatttc caccgggata atgctcacac
    17701 cacgatcgtt ataaattttt acgctatctc cttcgctaat cccgcgctgt tttgcatctt
    17761 gcggattcag ccagagtttt tgcgtctgaa cttcttgcag ccacgggtta gcatactggg
    17821 tggaattagc acggtttttc cctttccagg tgatgagctg caacgggtat ttcgccgtca
    17881 gtttatcttc tggcccttca aaggcaggga catagtgcga aagcgcggga atttctggat
    17941 cttgcatatc aaaaagacgc ttagagaaga tctcaatttt gccggaaggc gtcgggaaag
    18001 gattattctg cggatcgcga atatttgctt cgaaagcgat gtgcggcgcg cttttgaaaa
    18061 gatgtcgacg tgttttttgc agggttgcga agtccggcaa attttcatcc ggcatcgcca
    18121 gacgagtttg ctcccagata tgctcgatcc attgcttctc atcacgtcct tgactgaact
    18181 cggcttcgac acccagtttt ttcgccacgt cacgcaacca gtcataatcg gtgcgacgtt
    18241 caaaatccgg ctcaatcggt ttttctgaca gaataagata gctcgcagtt ccccatgttt
    18301 cgccgatatt ccagcgttcc ataaagctgg tttccggcag caacacatcg gcatatttcg
    18361 cgctcggagt catgaacaga tcgctgagta cgatgaattc tattttagat tcgtcttcaa
    18421 ggagcttcgc ggcctggtgg acgtccgggt tctggttcgc cagataatta cctgccagtg
    18481 agaacagcag gcggatatta ctgtcgagtt tatcgacacc tttaagccca tcttgcggcg
    18541 taactttaga agcatcatcc gccgcctgca tccagttcat aatcgagatt ttcgcttgta
    18601 ccggattttc tggcatatct ggtccaacgc agaatttacg attagaacta ccgccgtaac
    18661 ctgccgccca gccacctttg atgccgacgt taccggtaat cgaggccagt aaagttgaac
    18721 ctcgggcggt gcgttcgccg caaatatgtc gctgtggccc ccatccctga atcaacgctg
    18781 cgggttttgt agtggcgtaa tcgcgtgcca gttgacgaat ggattgtgct ggaacgtggg
    18841 taattttttc tgcccattcc ggcgttttgt gtatcccatc ttttgcgcca aaaaggtagg
    18901 caaccaacga ttcgttggct ggtacgcctt ccggcatgga gttttcatca aaaccgagag
    18961 tatatgtgtc gataaatgtc ttatcgtgga gattctcgct gataatgacg tacatcatcg
    19021 cgtccatcaa cgcattgtcc gtggtcggca acagcgggat ccactgatcg gcaagtgaag
    19081 atacggtgtc ggaatagcgc ggatccacca cgataaaacg tgtaccgttt tgcttcattt
    19141 tttgaaaata gtaattggtg tgaccgaata tggtttccgt tgggttatgc ccccagagaa
    19201 tcaccagttt ggtatccaga agagtatcca tcgagttacc gctcgcggcc acgccatacg
    19261 tgtaaggcgt ggccgccgca gtattaccca gactaactga gtgataatat tcaagataac
    19321 caccggttaa atttaacagc cgacgcgcca tcgcatcgcc agaaaatgtt ccaccagaaa
    19381 cagccgtccc gacatggaca taacgtgaag ctgggccata ttgttgcgtg atccttttca
    19441 ggttatcggc aattaaggtc gtcgcctcat cccatgagat acgctcaaat ttaccctcgc
    19501 ctcgtttacc tacccgcttc atcggatatt tcaaacggtc tggatgataa acaaatttac
    19561 gatagccgcg accacgaaca caggcgcgca taatcggcat ttgtggatcc aattcgttat
    19621 cgggaagcgt ggtaatttgc gtcaccacgc cgtcgcgcat atgcgcccga atatcgcact
    19681 taccaccgca gtcaaatgtg ctacaggtgg gcacgacttt atcagctact ggagctgtaa
    19741 cttctggcga cgcatctggg gattgcgcct ttgcggtaga agaaataaaa ggcaaagtga
    19801 taagcgcgga agtcgcttgt aaaaaactac gtcgtgatac ttgcggaaat gtaatgcgtt
    19861 ccttttcgtc tggcgtatcc atcttgcgtt cccgaaaatg aaaaaataaa tattcacgta
    19921 tttattcgag aacaatgctg ataacaatca aataattagt cgtttaaaaa aacgtgagat
    19981 tattaaatta ttaatgcatt ccaaatataa ataccataat tagattaata aatattttgt
    20041 atatttcact gaaatgaaaa atgtactgac gcgatttgcc ctacgtcatc catcaccagc
    20101 aattgataat cacctttatc cgtcaaatgc agggtcacgt tgcgcccgcg ttcagttaac
    20161 ggttcgccat tcagaaacca ccagcgttca cctgcccctc cacttgattg caacggcaaa
    20221 gtcgcttccg ctgcgcccgg taaacgttta ataatctcgc catcgcgaac gcctgtcagc
    20281 tgcagcggga gctgggcatc gtgaccgtat ggcgggcaaa ttgtcgaggc cggtggtaag
    20341 cgcacagcgc gacgttctga tgcgggcaac cagggttcca gcggtagcgg ccagacattt
    20401 atcatttctt gtcgcgcttg cgggcaatca gcggcaacac gtttgccatt ttcatccagc
    20461 cagatgggga aacgaatgcc attaatgcct tcctgctccg gcagtaatag agttggcggc
    20521 tgactcccgt ccagcagcca ggttgccagg cggcggcgac agttaccgtc accttccggc
    20581 aaagactgtc cgcccggcca gcaaataacg ccacgagtga ctgagttcgg gcgcgggtct
    20641 tccggcagat tagcactgcg cgacagtaag atattattga cctgattcaa caatggtacg
    20701 gcgctggcaa aaccaaactg accaacaacg ggcgtgccgt ccggtctgcc agtccagatc
    20761 ccaatgacat agcgagcgtt aaccccaatc gcccaggcgt cacgatagcc atagctggtg
    20821 cccgttttcc atgccagtgg ggcgacgcgc ggcaaggcac catccggcaa gggtagcgct
    20881 tcatcagcca taatccggcg aatgatccac gccgctcccg acgacattaa aggccgttca
    20941 agcagcggat cgtcaggctg taagcgcaat ttgcctgcct tgccgtggcg agcaaacgcg
    21001 gtatacgctg ccgccatatc ttccagtttc gcaccagcgc cgccgagaat gagcgaaaga
    21061 ttcggcgcag caccgttggg caaatataac ggcaatccaa cattgcgtaa ctttgccgca
    21121 aaccgtttcg gtccataggc ttccagcacc tgcacagcag gtaaattcag cgagcgcacc
    21181 agcgcctcgc tcatgctgat cgggccatga aaaccgctgt caaagttgcc aggtcggtaa
    21241 tcaccggtac gccgggggac gtcttgcagc agtgatgccg ggtggatcaa tccttcatcc
    21301 agcgccagac cgtaaacaaa cggtttgagc accgatcctg gcgatcggat cgcattgacc
    21361 atatcaacat gaccaaagcg tgaatcatcg ttgagatcaa ccgaacccac ccagccgcga
    21421 acacgcatat cggtatgatc aaccacgatc atcgccagtg agctgcgcgg tggtaatcgt
    21481 cctttccagt tttgcgccag ttcttccaga cgtcgttgaa gaccggcatc cagcgtagta
    21541 acgattttgt cgcttttgct tttaccgagc atcatgcgcg aaaacagcgg tgccagttgc
    21601 ggcatttgtc ggggtgccag ccagatgggt tcttcccttg actcttttac ctgctcacgg
    21661 gaccacacac cttgcacggc catccgttcg agcactttat tacgcgcgac ttcggcacgc
    21721 tccggccaac gatccgggcg aagacggctg ggcgcttgcg gcaaaaccgc cagcattgcc
    21781 gcctcggaat agcttaaatt cgcaggcgac tttccgagat aagcccaact tgccgcaccg
    21841 atcccctgca acgtaccgcc aaacggagcg cggttaagat acaaggtcag aatttcacgc
    21901 ttagacagat gccattccag ttgcaacgcg cgccagagct ggcgaatttt gccgccaaat
    21961 gttttggggt gaggatcaag cagacgagca acctgcatag tgagcgtgct gccaccggaa
    22021 ataacccgtc ccgaagtgag atcttgccat gctgcgcgcg ccaccgagaa tggattcacc
    22081 ccaggatgct tccagaacca gcgatcttca taattgatca gcgcttcaag gtaacgtgga
    22141 gaaacatctt cgattgttac aggataacgc cagatgccgt cagcatcggc gaagcgccag
    22201 agcggcgtac catcctgcgc cacgaccact cgtgcgggat tgacttcatg cagcggtaga
    22261 ggccagagtt tatctgctcc ccacgctgca agaaaaagga gaaatggcgc agctgccagc
    22321 gttatccagc agccgcgttt ggttaacaag cgaggcattt acggtctgac aatcagcaaa
    22381 tcttcagccg cgccggtcgc ccgccattgg ggaacataca ttgattccac catcggttgc
    22441 ggtacctgat acgttcccgg cgtcaccgcc cgcgccagat acaccagcgt taccggttgg
    22501 tattcatcaa cggcaaccgc cgccacaaag cgatcgtcac ggaactcaat gtgcttaata
    22561 ctcgcctgct gcatctggtt cagtaagttc tgcacttcgc caccactttg ctccaggctg
    22621 gcgctaccgt tcgccagatt ctggttttcc agttccagac ccgcaggcag cagatccacg
    22681 actaacgcat ccggcacgct gttactagct tttacctgca accagaccag caccagatcg
    22741 ccgctacgta acgagtccag cgatttgctc ttaccatcag taccaagaat atgacgctca
    22801 atttgcagca cattgttcgc cggtaaaggt gcggattgcg gataaccgct ggcatccaca
    22861 cgcagccata acggctgctc gccactgttg gtcacctgca aggtggcaag ttgatcgcta
    22921 ttcagattgc tgttttgcgc tttctcgcct gtcagcggct cagctgagaa agaggtttgc
    22981 gcctgccatt tacctggtaa atcctgaatc gtacgggcag ccaggaacaa cgcgttactt
    23041 tcctgcgtcg atagccagcg ttcaccaaac gcctgctgcg aaagtgtgtt cagcagggag
    23101 tactgctcat cgggtagcag tttgttctct tccagcaagg agagcatcaa cgcgttgtcg
    23161 cgcagtggac taccgtaatc acccagccat atccgctcat cactattacg cggcgttttt
    23221 agcgccagcg caatcgcttc ttcatcacgc gtcgcatcac ccatggtttt cagcgcaacg
    23281 ccaagttgca gcagcggtaa accagaagcg gcatctgcac gatgctccca gatttcacgc
    23341 agcgcaccca gcggagcctt ttgctgacgg gccaacacca gcgcagcgta agactgtacg
    23401 gcgaatttac tggctttgag attatccgcg tacgggatcg acatcatgcc cggatcttgt
    23461 aaatagcgca gcagacgctc attaccccgg ttaatggcgt ctgtcggcac gctgtaaccc
    23521 tgttcgcctg cgcggaccag gaaatccatc acgtaagccg tcagccagta ctcttcgtca
    23581 ccgtttttat cccacagcgc aaagccgccg ttatcacgtt gcatttgcag caaacgggaa
    23641 atgccgatat cgaccgatgc acggcgtttc tcatcactgt cgcctttgat gcccaacgcc
    23701 tgcagttggg ctgcgttggt ataaagtgac gggaacaggc cgctggcggt ttgctcaaga
    23761 cagccgtacg gatacgcttt taactctttg atataacgtg cgatgttcag cggtggtttg
    23821 ccgctcaaca acaattgccc ttccagcgta acaggcgaga agttttgcaa tccatccgcc
    23881 ggaatcgccc atgtctcacc aggctgtaac gccgtaccgt aattaaccgt ttgtgccggg
    23941 aacgccggac ggacgccgat tttccactgc ttatgctgat cggcaacggt ttcacccggt
    24001 aacgctaacc cgctaatggt ggcctgaatt tctccatcgc cataacccgg caatgctcgc
    24061 accgggataa acagcgtagt acgcacacct ggcgctaatt caacggctgc gggtgaatcg
    24121 ctgaccagtt caagcaaacc actggcggtc agggcaacgt tcagtttttg cggtttatcg
    24181 gtaagattag tgatatccag cgtcagacgc gagttatcgc cactcgccat aaagcgcggc
    24241 atgttcagtt cagcaatcac cggtgcggca actatcactt tgctttcgtt gctaccgaat
    24301 tcatctgccg tccaggcttg cgccatgacg cgcaattcac cgttaaaatc gccaatcggc
    24361 agtgtaaccg agccttcgcc ctgttcgttg agcgttaccg gcagcgcctg ctgcgcgaca
    24421 atattgacgt gattgaccgg cggtttacca ccacgtttca gctcatcacc atcgccaccg
    24481 aaacgcagag ctgccagacg cccctgacct tcaataacct gaccgtaaat atcgtaaatg
    24541 tctgcgccat agcgtttctg accaaagaac gcctgccacg gatctggcgt gacgtagtca
    24601 gtaatattca gcacaccact atcaacggca gacaccagca cattcacctg tttaggcttc
    24661 tcgccatttt tagtgctggc tttaattttc acggttaatg gttgattggg acgcattttt
    24721 gctggtgttt ccagcgccag atcgaggcga cggttttcat caccaagcgg cagatgcaac
    24781 acaccaaccg cgcgttttgg cgtcgcggag cgagatttat cgccaggacg caccaccagc
    24841 gtactcaaat aaagatcatg acgattccag gttttatcga ccggaatcgt cagatccagc
    24901 ccttgagccg gaacatcaat ctcttgccac cacagcggcc cttcactgga ctcgaccatc
    24961 gcataacctt tacccgccgt tggcgcggcg atatgcaact taatggtatc gccagggcga
    25021 taaccggctt tatccagttt cagcgtgaca cggtcgggtc gcactgcgcc gctaccgtcg
    25081 ctattatcct gccagctata gccagcccaa aaacgaacac tactgaccgc ttcattcggc
    25141 gctttgactt ccagacgata agcgccccac tctaccggga aactgacctt gccggtttcg
    25201 tccgctttca gatccagagt ttgttcattt tcaatcagat ctttttgatc aaactgtgat
    25261 tgccagcctt catcttctga ccagttccag tagtaatcgc ggcgttcgcg aatcaggcgc
    25321 acctgcaagc ccgacacggc ttttttcacg ccttgcgcat cgctataaac gatgtcaaaa
    25381 gcggcgttac tgccttcatc aacaatgggc tgtttcaccg tgctgtcagt gcgataatcg
    25441 taaaccgatt tcgaggcgaa ctgcggacgg atccccggca atgcatcggc aggccagata
    25501 gcctgctcag cgcggcgcgt caccgggcga ccgcccgatt ccagcaaact gccctggaaa
    25561 ataacctgca atggggaatg cgtttccttc cactggcttt ctgtagaaac ttcgccgcgc
    25621 cctttatcat ccagcgtcaa ctgaacttca tccagcgtgc gggaaaggtt ttcggcagct
    25681 atatcgccga attcaaaacc aggtaaggct gacacagcct cacgcagtgg gcgcaggaaa
    25741 agttgccctt gcaaagtatt accattagca ggcgcaccgt acaggtagta tcccaccacg
    25801 gagaatttca cttcatcatt cggcgtaagc ggggttttct caccggtcag attcagcgcc
    25861 atacgctctg gcataaaatc ttcgacgtgg aaatcccaca tccgatactg gttatcgccc
    25921 gtgttagcgc gaatatgcca cataccggtt gccgcattgc tatcgagtgg ccaggtaaag
    25981 tggtagaggc cattttccgg ctgactaacg acgctcctga gtacctgccc atcgggttta
    26041 atcacgtcta acttgatggg ttgattgggc aacgctttac cgtctgcatc acgcagcaaa
    26101 ccattgagga ttaccgtttc acccgggcga taaagatcgc gcgggccaaa catgaaaaac
    26161 tgtttgctat agcctggcgc gccagcaatg ttaaattctg ctaagtccag cgccggaagt
    26221 tttaaatcga gtagcgttgt ctgaccgtct ttacgtgcca gcaataatgc ggcgttttta
    26281 tcattttcca gctgcacatg cccctgagcg tcactggttg cctgagtcag agtctgccct
    26341 ttctcattta ataaagagac ttcaattcct tgctgggccg cgccgttttc cagactttgg
    26401 gtaaagatat ccagacgatt gtgataacgg tgagctgaaa cgccgatatc acttaacgta
    26461 aacagcgtcg cgggattact gtaatcgtaa cgtccagcct gattcatcac agccagatac
    26521 acgcccgcct gctgaagcgg tttgatatcg cccagcggca gcaataattt ttcacgggtg
    26581 ttacgcgcag gattgagatc aaaccgtccg gtgtagacca gatccgccat ctgcagcagt
    26641 ttgtctgact gccagttcgc cagcgaattg cggtattccc attggctaat gaatgctggc
    26701 agagattctg gcttaacgcg gaagaagtta acatcgacat tattaacgtt gagcgccatt
    26761 accggcagcc cttcaacgac tttaccaggc agcagcgaac cacggctggc aaaaccgaca
    26821 ctgggttgga tgtcgcgggt agttatagtt ttttcgtaat ctttactgaa ggttgcgttg
    26881 ttgagtgctt tgacctcctt gccaattgta acgatcaaat cacgtttcgg ttcgaggtgg
    26941 cgtaaacgca gctctttaag attatctgat agctcccagg caccatccac tttgccgctt
    27001 tttttatcga cgacatgaat aacgcgtgag aaatcctgat ccgggtcgag agggatggag
    27061 aacgtcagca ccagcgtggc tgcaccatca agttggactt cagagagatc cagcaatgtc
    27121 agcgccttac cggcactctg ttgggccagt ttttgtcgct ctggcgcgga gagttttgct
    27181 gaactcgcgt tttctgaaga ggccgcttta gtaacttcag aaggcgcatc ttttttcacc
    27241 gctgttggcg cgttatcgtt gttgtcgcac cctgccagcg ccagcattag catgcaggcg
    27301 gctacgcgta actttttcat ttttcatccc tgccacaatg gcccgttagc aacgtcgaat
    27361 aattattatg cgtgagaatt tacgttatgt aattcagtat cacgcccata caatccgaat
    27421 tttagtgaga attgtggctc agacagccaa atcagcctct tttgcttgtc gcttttcact
    27481 aaacatccga caatttagcc taaccccggc aaaaatggag atgcctatgt ccacgacatg
    27541 gtttgtagga gccgactggc tcgccgaaca tattgatgac ccggaaattc agattatcga
    27601 tgcccgcatg gcgtcgcctg gacaggaaga tcgtaacgtt gctcaggagt atctgaatgg
    27661 acatattccc ggcgcagtgt tttttgatat cgaagcgctt tctgatcaca cttccccgct
    27721 tccgcacatg ctgccgcgcc cggaaacgtt cgccgtggcg atgcgtgaat taggcgttaa
    27781 ccaggataag cacctgattg tctatgacga aggtaatctt ttctcagccc cacgagcatg
    27841 gtggatgctg cgcacctttg gtgtagagaa agtgtcgatt ctggggggtg gacttgcagg
    27901 ctggcagcgc gatgatctgt tgttagaaga aggtgcagta gagctgccgg aaggagagtt
    27961 taacgccgcg tttaatcctg aagcagtggt gaaagtaacc gatgtgttat tggcaagcca
    28021 tgaaaatacg gcgcaaatta ttgatgcccg cccggctgca cgttttaacg cagaagttga
    28081 tgaacctcgc ccaggtttac gtcgcggaca tattcccggt gcattgaacg ttccgtggac
    28141 ggaactggtg cgcgaaggcg aactaaaaac gaccgatgaa ctggatgcga tattttttgg
    28201 tcgcggcgtc agctatgaca aaccaattat cgtcagctgc ggctctggtg taacagcagc
    28261 cgtggttttg ttggcactcg cgacgctgga tgtgccaaac gtgaaactgt acgacggcgc
    28321 atggagtgaa tggggcgcgc gggcagattt accggttgag ccattgaaat aagcttttta
    28381 caggcaataa aaaaccgccg aatttggcgg ttttttattg ctggtctggt tcgcggcctt
    28441 tccagcaggt tgacttgtgt tgccggagca acgcaggcac cccacagtgt tgaaatactc
    28501 tcttttatgc ctggctgtgt caagataata aacagagtaa ttattcaaac atgagagtat
    28561 tattcttaat ggcccttttc gccgtctcgc aaacgggcgc tggctttagg aaagaatgtt
    28621 ccatgaccgt aaatacaggc accccacagt gcttgctatc gcggcaatat cgccagtggt
    28681 gctgtcgtga tgcggtcttc gcatggaccg cacaatgaag atacggtgct tctgtatcgt
    28741 acttattgtt tctggtgcgc tgttaaccga ggtaaataat aaccggagtc tctccggcga
    28801 caatttactg gtgattaaca accttcagag cagcaggtaa gcccgaatgc cgccctttgg
    28861 ggcggcatat tttagattat ccgattctgt ttaaagtcac gcaaaaaacc accccagcga
    28921 cgttcataga atggcgcaat atgttcggta ataaagtggc taattccttt ttcccctttt
    28981 ttcacctgac aaatatcgat tggttcatcg ccaggtaatg tatcggtcgc tacacttccc
    29041 gccacctgaa taatttcttc gatatcacca tcggcttcaa tgccaataag taaattaggc
    29101 tgtgcctctt cgttctcttt aattgaacaa ataaaagcac gcttcacagg cttaatggtt
    29161 ttaaataagg tggtgagtga atcaatcatt tgtgctggcg gctctgcgac ttccgataat
    29221 atcagcgatt caccgccttc caggatttcc tggctgctca gcggatttcc ctcttcacca
    29281 atcaacaaac tgatttcacg cggcataaat tctttaccgg tcggcagttt ggcattaagg
    29341 aagagcgttt cgccaagggt catctcaaac aatgtgcgaa cgggcattac gacaaatgcc
    29401 tgttcgtctt caaccgcctg ttgaagtgct tctaacgagg taaaaaaagg aatgacgctg
    29461 gtgccgtctt ctttttccca gtgctgtaaa tcaagcgcgc tatcttcaac cacagcctcg
    29521 ccctgcgccg ccgtaccagg cacccagacg gtggattcca gtagagtacg gaaaaaggcc
    29581 gggcggtgcg ccggttcggt tgctgctttt tccagcaggt cttcaagttc attttttgtt
    29641 tcggacataa gaaccacaat tcattcaacg ttcggggcac aagtgcctga tgcgccatac
    29701 ttatcaggcc tacaagggat tcacaatttg ttgaatttgc aatactttgt aggccggata
    29761 aggcgtttac gccgcatccg gcatattagt ttacgccgtt aacagattag caatcgtgcg
    29821 cacaccaagt cccgtagcac ccgcagacca ctgttcaacc ggcgctttac ggtaagtcgc
    29881 cgagcagtcg atatgcagcc agccttgctg atagttctca acaaagtgcg acaggaagcc
    29941 cgctgccgtg ctcgcgcctg ccggatacgc cgcgcttccg gtattgttca gttcggcaaa
    30001 gttagacggc agctggctgc ggtggaattc cgccagcggc agacgccaga atggttcgtt
    30061 ctcttgtgag gcactcgcca gcaaacgacc ggcaagcgca tcgtcaaaac tgaacagcgc
    30121 gtgataatca ttacccagcg cagttttcgc cgccccggtg agggtcgccg catcaatgat
    30181 catttccggt ttctgcgcac tggcatcaat cagaccatcg gcaagcacca ggcgcccttc
    30241 ggcatcagtg ttcatcactt caactttttt accgttgcga taagtgatga tatcgcccag
    30301 cttgaacgca ttgccgctaa tcaggttatc cgcacagcag aggaacagct tcacgcgctt
    30361 gttcagtccg cgcgtaatgg caaatgccag cgccccggta accgttgccg cgccgcccat
    30421 gtccgacttc atcgagtcca taaacgcagt ctgtttgatg ctgtagccgc cggagtcaaa
    30481 agtgatacct ttacctacca ggcacgcgta cactggcgct tctttatcgc cagttgggtt
    30541 gtaatccagc gccagcaata ccggagaacg ttctgaaccg cgtccgacgg tgtgcagccc
    30601 catataacct tgctcacgca gatcttcgcc tttggtgatc cgataagtca cacgatcgct
    30661 cgcgacgttg ctgatcagat caacagcacg ctgtgccagt tgcgatggtc ccaattcttc
    30721 tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc atcaggcggt tatccagttc
    30781 ctggcgctgg gcatcgtcca gatccggcca ctccacttta cgcgtgcctt tcggggcttt
    30841 gtaaccttgc cagaatgccc agcatcgatc cgcatcccag ccttcaccgc ttaactgaac
    30901 atgcttgatg cccagaccgt caatcttgcg cgccgcacgc tggatcagcc ccagatcgtc
    30961 tgccccgttc aaatgcaggg taatgccgtc attattaatg ctgtaagttg ctttttctcc
    31021 ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc atcgcttctg tcattttagt
    31081 tatccttctt tgtaaaaggg ccgccagttg gcagccccag gatttttccg ctaaatgatt
    31141 cgcgttgcag gaaggcggca agtgagtgaa gccccaggag catagataac tatgtgactg
    31201 gggtgaacga gcgcagccaa cgcatctgcg gcgtgaagca tgacgcggaa attattcggc
    31261 ctcgtccagc cagactaaca aaatcgcttc gaggattttc tcgttggatg cctgcgggtc
    31321 gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt
    31381 cggatcaaga tcgggatacg catcgtacag tgcttcgcca atttcgcggc tatcggtcca
    31441 cttaagtccc atactaacct ctgttaatgc tcacgcgcat ggttgatcgt gtaacgcggg
    31501 atttcgacca ctaaatcttc gtcggtgacg cgcgcctggc agcttaaacg gctttccggc
    31561 tccagtcccc aggctttgtc cagcatgtcg tcttcctgct ctgagctttc tggaagcgag
    31621 tcaaaacctt cacgaacgat gcagtggcag gtggtgcaag cacaggattt ttcacaggcg
    31681 tgttcaatct cgataccgtt acgcagagct gcgtcgagaa tggtttcacc gctattagct
    31741 tccagaacag cgccatcagg gcagagatcc tgatgaggca aaataacaat ctttggcata
    31801 ttaaacctcg tccacggaat ggcctttcag cgcacgacga accgactggt ccatgcggcg
    31861 agcggcgaaa tcctgggttt gtttgtctac gtttttaatc gcttgttcga tggcgtcaac
    31921 gtcatcgccc tgcgccactt cactcaggtg agcggcagca tcgtcaatga cctgacgttc
    31981 tgcggcgctt aacagcgcgg catcagcagc cagcgcgccg tgcagacttt ccagcacacg
    32041 cgccgcttct actttttgtt ctgccagcat tcgggctttt acgtcctgct cggcatagct
    32101 cattgagtct ttgatcatcg aagcgatttc gctatcggtc agaccgtaag acggtttgac
    32161 ctgaatagac gcctcaacgc cggtggattt ctccatcgcc gtcacgctca aaagaccgtc
    32221 ggcatcgacc tggaacgtca cgcgaatatg cgcaccgcca gccggtagcg ccggaatacc
    32281 acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc agttcgcgct caccctgcat
    32341 tacatggata gacatcgccg tctgaccatc tttaaaggtg gtgaaatcct gagcgcgggc
    32401 cactggaata gtggtattac gcggaatcac tttctccacc aggccgccca tcgtttcgag
    32461 gcccagcgac agtgggatca catcaagcaa cagcatttcg ctgtctggct tgttacccac
    32521 cagaatatcc gcctgaatcg ccgcgccaat agcgacgact ttatccgggt cgatggaagt
    32581 cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc accagcggca cgcgagtaga
    32641 accgcccacc atcaccactt ccagcacttc atcagcttct acacccgcgt ctttcagcgc
    32701 gcgacgacaa gccaataagg ttcgttttac cagtggcgcg atcagttcat tgaattgttc
    32761 acggctgatt tcgccctgcc agcccgcaac gttaacggtc acggagtccg catcgctcag
    32821 cgcgattttg gctgcaatgg cggcatccag cagttcacgc tgaacgcggt tatcgctacg
    32881 atcaggaatg cccgcctgct cgcgaatgta atccgccagc agatggtcga aatcatcgcc
    32941 gccgagcgcg gaatcaccgc cggttgccag cacttcaaac acgccgcgac ttaagcgcag
    33001 aatggaaata tcaaacgtcc cgccaccgag gtcataaacg gcgatcacgc cttcctgacc
    33061 ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg ttaagtaagc gcaggacgtg
    33121 aaggcccgcc agacgcgccg cgtctttggt gccctgacgc tgggcatcgt caaagtacgc
    33181 cggaacggtg ataactacac catccagctc gcctgccagg gcttcagttg cccgcgccgc
    33241 cagtgctttg aggatgtccg cagaaacgcg caccgggttc agcagccccg ccgccgtttc
    33301 aatcatcggc aggccgtttt cgctggcctg gaattgataa ggcagatgcg gatagcgttg
    33361 ctggatatca gccagcgagc gtcccatcag gcgtttaaca gaactaattg tgttggcggt
    33421 atcgagcgct gcattagtac gcgcgtcata acccaccgaa tgcccttgct gttgatagtg
    33481 aacaacagat ggcagcaggt gacggccttc atggtcggct aacgtttcgg cctgaccgct
    33541 gcgcactgtc gccaccagcg agttggttgt gcccaggtca ataccggccg ccagacgacg
    33601 ctgatgcggc gcagcactca aaccaggttc actaatttgt aataaggcca tgtttagctt
    33661 ccagaaatta aaaatcgagc agtttttctt cgagttgttc ggcactgctt cgcagtttat
    33721 cgagaaaacg cagcttacgc acggtatccg ccgccgcgtc ccaggtctcg ttgtctaact
    33781 gttcaaccat caactgatgg cgggtatcaa acatcttttt cacacgtttg ataaagcttt
    33841 ccagccgcgc ttcatctttc gcctgttcga tctcgtccag ctcttcgcgc agctccaact
    33901 gttccatcag gaacgcggtg tcgcgcacag tatgctgctc gctggcgaga tcaaagccgt
    33961 gcaaagaaag caaatattcc gcgcgcatta acggatgacg cagcgtttgc caggcctgat
    34021 taatggttgc agattgctgt acggcggcga gttgttccgc ctggcttccg ctggcgaatt
    34081 tatcaggatg atactgacgt tgtagatcct gaaaacgcag gctcagcgcc tgggtatcga
    34141 gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc cataacattc tcagggctgc
    34201 gatccgccgc gataacggcg aatcaggtaa aacaaccccc acgcgcaggc gaccacggtg
    34261 gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg caaccacact catctttaac
    34321 gttcgggttg gtgaatttaa acccttcgtt caggccttct tttacgaagt ccagctgcgt
    34381 accgtccaga aattgcaggc ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa
    34441 cacgatgtct tccggcgtcg gttcgtcaac aaattccagt acataagcca tacctgaaca
    34501 tccggaggtt ctcacgccca gacgtaggcc aaaccctttg ccacggttag ccagaaaggt
    34561 atttactcgc gctgctgcac tgtcgctaag tgtaatcgac ataaccaaac ctcaactctt
    34621 attttgcttc acgtttgctt ttatagtccg caatggcggc tttgatcgcg tcttctgcca
    34681 gaatagaaca gtgaattttc accggcggca gttcaagttc ttcagcaata tcggtgtttt
    34741 tgatagcctg cgcttcgtcg agagacttcc ctttcaccca ttcggtgacc agggagctgg
    34801 aagcgatagc agaaccgcag ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat
    34861 cgttgacttt aatctgcaac ttcattacgt cgccacaggc cggtgcaccc accatgccgc
    34921 tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt acgcggattc tcgtaatggt
    34981 cgataacttt ttcgctgtaa gccattataa attctcctga ttccgatacc gattaatgat
    35041 gagcccattc gatgctgttc agatccacgc cctgcttgta catttcccac agcggagaaa
    35101 ggtcacgcag acgaccgatg gatttacgaa ctaactcgat ggtgtagtcg atctcttctt
    35161 cagtagtaaa acgacctaaa gagaaacgga tagagctatg tgccagctcg tcgttcagcc
    35221 ccagcgcgcg cagcacgtag gacggttcga ggcttgctga cgtacaggcg gaaccggaag
    35281 aaaccgcgag atctttcagc gccataatca gcgactcacc ttcaacgtag ttgaagctga
    35341 cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat
    35401 ctttgatgcc gttccacaga cggttacgca ggccgcgcag acgttccatc tcggtcgcca
    35461 tctcttcttt tgcgatgcga taggcttcgc ccattccgac gatctggtga acaggcagag
    35521 tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat ttgcgcttcg atgcgtacgc
    35581 gcggtttacg acgcacatac agcgcaccga tacctttcgg gccatagatt ttgtgaccgg
    35641 agaaagacat caggtcaact ttcaactggc tcaggtcgat aggcagtttg cccacgctct
    35701 gggttgcatc aacgtgatag ataatgccac gagcacggca catttcgccg atagccgcga
    35761 tatcctgcac cacgccgatt tcgttattta cgtgcatgat agaaacaagg atggtgtcgt
    35821 cacgcatcgc tgcttcgagt tctttcaggt cgataatacc gttacgctgc ggtgcgaggt
    35881 aggtgacttc gaaaccttcg cgctccagct gacggcaggt atccaatacc gctttgtgtt
    35941 cggttttgct ggtgattatg tgcttgcctt ttttctgata aaagttggct gcacctttga
    36001 tcgccaggtt gtcagactcg gttgcaccag aggtaaagac gatttcacgc ggatcagcgc
    36061 cgaccagatc ggcaatctga ttacgggcga tatctaccgc ttcttcagcc tgccagccga
    36121 aacggtgaga acgggaggcc gggttaccaa aggttccgtc catcgtcata aactgcatca
    36181 ttttctcggc aacacgcggg tccaccggcg tggttgcgga gtagtcgaga taaatcggta
    36241 atttcattgc tctataaact ccgtacatca ctcaatgcaa ggaaccaggc taccggctgg
    36301 atgtacgacc gtgtttacgg agcgcgatac gccccggcct gattctgaat actttttatt
    36361 aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt gcgtggcgcg tcgtgagtat
    36421 gctgacgacc agacacatcc agcacttcct ggttgttaac cagttcgcct aaagtaatgt
    36481 tattgagaaa accggtaaga cggtcgctca aatcacgcca cagcgcgtgg gtcaggcatt
    36541 tatcgccgcc ctggcagccg cctttaccct gacaacgggt ggcatctaca gattcgtcaa
    36601 cggcgctaat cacttcgcca acggcgatgc tgctggcatc tttgcctaac agataaccac
    36661 cgcctggtcc acgtacgctg gaaaccagac catttttacg cagacgagaa aacagttgtt
    36721 ccagataaga aagggaaatt ccctgacgtt cggaaatatc agccaacggt accgggcccg
    36781 cttcagagtt gagcgcaacg tcaagcattg cggtcacggc atagcgccct ttagatgtca
    36841 gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg ggttttttat tgtaaaagtg
    36901 ggggtattgc atagcagggt caagtctgac attcccgagt aaattggtca actatttact
    36961 tgactgattt agtcgggtat ttaaccttca gtgccatttt tttatcgtgg cgtttgtacc
    37021 tgttatcctt ccagaccatg ggcgggttga gggcaattat tcgcattagc gcaggcctgt
    37081 tcccctcacc ctaaccctct ccccgaaggg gcgaggggac cgtccgtgca cttatctggc
    37141 ttagccctca ggccttatta cctttattct gctgctctat agaagccaga atcccgcgca
    37201 ggatattcaa ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc aatttattca
    37261 tcacctgccc cggatggttt tcacggataa aaccggttgc cagcagcgtt tgttccagat
    37321 gaccataaaa acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgacct
    37381 gctcgccatt ttcctgagtt gccagccagg ccatacgcac ttcataggca ataacctgaa
    37441 ccgccatcgc caggttcagc gagctgtatt cagggttagc ggcaatcgcg acatgataat
    37501 gacacttctg caactcttca ttggtcaggc cgacacgttc gcgaccaaac accagcgcca
    37561 ccggggtatt tgccgcttca gcaacgcttt tcagaccgca ttcgcgcggg tcgagcatcg
    37621 gccacggcag cgtgcgggaa cgtgcgctgg tgcccaccac caggctacaa ccggctaacg
    37681 cttcgtcgag cgtatcgacg atgtgagcat taccaatcac atcgctggcc cccgccgcca
    37741 gggcaatcgc ctgggagtca ggtttcacca gtggattaac cagccacaga ttggttaatc
    37801 ccattgtttt catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca
    37861 gcacaattcg aatattttgc agcattgtct ttcttcgtct aaagattatt cgcgcatctt
    37921 atcataaaac gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct
    37981 ttaacatcca gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc
    38041 gcaaggcggg taatttgatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc
    38101 agaaaggcag taacgacttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg
    38161 acacgattcg taaatcttac ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg
    38221 aaggtactga tcaggatgtt caatgggtta tcgatccact ggatggcact accaacttta
    38281 tcaaacgtct gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag
    38341 ttgctgtggt atacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcagggcg
    38401 cacagctgaa cggctaccgt ctgcgcggca gcaccgctcg cgatctcgac ggtactattc
    38461 tggcgaccgg cttccccttc aaagcaaaac agtacgccac tacctacatc aacatcgtcg
    38521 gcaaactgtt caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg
    38581 cttacgtcgc tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgc ccgtgggact
    38641 tcgctgcagg cgagctgctt gttcgtgaag cgggcggcat cgtcagcgac ttcaccggtg
    38701 gtcataacta tatgctgacc ggtaacatcg ttgctggtaa cccgcgtgtt gttaaagcca
    38761 tgctggcgaa catgcgtgac gagttaagcg acgctctgaa gcgttaatga ctcaggcggg
    38821 tgatatcact cacccgccct cgcctttcag gcgctattcc gaaatacttc ctcaccgctt
    38881 tactttcttt cgtcactctc ccaccatctt tccccgatta atggatgata aagaagtcgc
    38941 aaggaaaggt tatggcactg ccagtgaaca aacgcgttcc caaaattctg tttattctct
    39001 ttgttgttgc cttctgcgtt tatttagtgc cgcgcgttgc catcaacttc ttctattatc
    39061 ctgacgataa aatttacggt cccgacccct ggtcggcgga atccgtcgaa tttacggcta
    39121 aggacggtac tcgtctgcaa ggctggttta ttccttcttc gacgggccct gctgacaacg
    39181 ccatcgcaac catcattcat gctcacggca atgccggaaa tatgtccgcc cactggccgc
    39241 tggtcagttg gttacccgag cgtaatttca acgtttttat gtttgattat cgcgggtttg
    39301 gtaaatcaaa aggcacgccg tcccaggccg gattgctgga cgatacgcaa agcgccatca
    39361 atgtggtgcg ccatcgcagt gatgtaaacc cacaacggct ggtgctgttc gggcagagta
    39421 ttggcggggc gaatattctt gctgttattg gtcagggtga tcgtgaaggc atacgtgcag
    39481 tgatcctcga ctccacattt gcctcttatg caaccatcgc caaccagatg atccccggca
    39541 gtggctactt acttgatgag agttacagcg gcgaaaatta tatcgccagc gtcagcccga
    39601 tcccgctttt actcattcac ggtaaagctg atcacgttat cccatggcag cacagcgaaa
    39661 agttgtatag cctggcaaaa gagccaaaac ggttgatcct aatcccggat ggcgaacaca
    39721 ttgatgcttt ttccgatcgt cacggcgacg tttatcgcga acagatggtg aactttatcc
    39781 ttagcgcgtt gaatccgcag aactaaccca tgatcgctag cacgataatc attcacaaaa
    39841 tcaccttaag acatgctaat ccactggtca ggacagttta agatgagaaa aattctgtga
    39901 cgcttgccaa catttctgat gattagcatt cccttcgcca tttccctgag caaactttag
    39961 ctattcttat caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca
    40021 tctgtccttt cggctcccca gcgccgctgc cagatcttgc tgacgctctt tcagccgggg
    40081 ttgaccgcca ccatggcaac cttcagcgag cttaatggtg tggatgatga tattgccagt
    40141 cttgatatca gcgaaacagg acgggagatc ctgcgctatc atcaactcac actgacgact
    40201 ggttatgacg gtagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt
    40261 cactggctac gacgtggttt ccgtctgtgt ccgtcattta ttaccagcca attcaccccc
    40321 gccctgaaga gtgaactgaa gcggcgcgga attgcgcgta acttttacga cgataccaat
    40381 ctacaagcgt tagtgaatct ctgctcccga cggctgcaaa aacgctttga atcgcgcgat
    40441 attcatttcc tgtgtctgta tctgcaatat tgtttgctgc agcaccacgc tggaattacg
    40501 cctcagttta atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg
    40561 caggaaattg gacgccactg gcagcgtcgg gcgctccagc ctgtaccacc tgatgagcca
    40621 ctgtttatgg cactactttt ttccatgttg cgggttcccg atccattgcg ggatgcgcat
    40681 cagcgggaca gacaattgcg tcagtctatc aaacgtctgg taaaccattt tcgtgagctg
    40741 ggaaatgttc gtttttatga tgaacagggg ttatgtgatc agctttatac ccacctcgcc
    40801 caggcgttaa atcgcagttt gtttgccatc ggtattgata ataccctgcc ggaagagttc
    40861 gccagactgt atccacgcct ggtacgcacc acccgcgcgg cgctggccgg atttgaaagt
    40921 gaatacggcg tccatctttc tgatgaggaa agtggtctgg tcgcggtgat tttcggtgcc
    40981 tggctaatgc aggaaaacga tctgcatgaa aaacagatta ttctactgac cgggaatgat
    41041 agcgagcgag aagcgcagat tgagcaacag ttacgcgaac taacgttact gccgctcaac
    41101 attaaacata tgtcggtaaa ggcatttttg cagacaggcg ctccgcgtgg cgcggcactg
    41161 attattgctc cttataccat gccgttaccg ctcttttcac caccgctgat ctatacggac
    41221 ctgacactga caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg
    41281 aacaactttc gggcgcaaaa acattgccgg aagcgccacc agcgccatca cccagaacac
    41341 gccgtggccc agatattgat acaggaaacc ggcgaaaacg gtcatgatag cgatactgcc
    41401 gcccatcgcg acggcagagt aaaccgcctg taaacggatg acttcgctac cctggcgagc
    41461 agcaatatag cgcatggcgg ccaggtggca gaccgtgaag gtgccgcaat gcagaatttg
    41521 caccactatc aaccacggca acgccgtagt tgctcccata atgccccagc gcactacgcc
    41581 gcaaatcgct gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa gtttattact
    41641 cagtgcaaag ataatgactt ccgccaccac gcccagcgac cacaaatacc ccaccgccga
    41701 ggccgagtag ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgtgc
    41761 cccctgcaat aaacaaacgc aggccagaaa gcgccagttc tggcgaacca gcgccaacca
    41821 cgctgaccaa ccggtgctct cctgctggcg gcttgcccct tgtggctgaa tcgtcggacg
    41881 gatgagaaag ccgagcagca tggatgccac gcccaacgtc aacagcacga ggatcacccg
    41941 ataatcaaac atagtgacca gtttgcccgt cagcgccgag ccaatgacaa acgccaccga
    42001 gccccacagt cgcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag
    42061 tgcatcggtc aacggtacca gcggtgagaa aaagaggtta aagccaatca tcaccagcat
    42121 cagccacgct acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg tcagcagtgc
    42181 cagcacgcgc aaggcggaaa tcaggcgaga aggatcgctg acgcggggcg cgatgagcaa
    42241 actcccgaga aaacgggcaa ccagacctgc ccccaataac aggccgatgg tttctggcgt
    42301 taaaccaatc cctttaagcc agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa
    42361 gtatgtgaaa tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg
    42421 tcaggaggcg ttagtctgga gttaatctaa tgttggtgca agttgaaaaa taagaatatt
    42481 cgagcctgct tcccgatgtc tgtaacgcca gttcacaagc actcaaaaac tgcgatccgt
    42541 gacgcatgtt gcaaagataa tgcgtaatta tgccgttacg cttgccaaac gttcctgcac
    42601 aatggcgatg aaatcgcgta atgccggctt catctcccct ttcttccacg ccatcagtaa
    42661 agcaatggaa ggcacatttc cggcaatggg acgaaaaaca acctgcccgg tattaaaatt
    42721 attcatataa ccgggtatca aagtgacgcc cagccccatg cccaccagat tcatggtcac
    42781 cagaatattc gttgccacct ggacgatatt tggctggctg ttttcttgtg caaaccacgc
    42841 tttaacgatc ggcgcaagcg aaccggaata cgccggatcg gtactgacga aattcacgcc
    42901 atccagttgc gcggcggtga tctctttttc atgtgctaaa gggtgatcaa ccggtaacac
    42961 caccactaat ggttcgtcaa aaagctccag ataatcaatc tccgggctat aaacaggatg
    43021 gcgcattaag ccgacgtcga gttcaccacg gcgaattttt tcctcctgtt gcgtggtgat
    43081 taaactcacc agctcaatca aggtgtctgg ctgtctgaga cgaaacatcg gtaatacttt
    43141 tggcagtaaa ttcacttccg ccgatggcac aaagccaatg gttaattgtc tgtcttcctg
    43201 aacaattttc cgcgcccgta atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc
    43261 ctggagaaaa cattctcccg ccgccgtcaa cgcgactttg cgcttatccc tcaccagtaa
    43321 cggaacaccg acacagtttt caagatcgcg gatctggctg cttaacgaag gctgtgaggt
    43381 atgcagtttt tccgccgcac gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg
    43441 taaatgccgt agttccatca ccttcccctt gttatcgaaa aaacgtctca gctggtagaa
    43501 attaactatt tcacatatta gcaaccaacc agcaacatcc ttatgacaca aaaatagaag
    43561 gtcaatacat cttatctttc aggattaaaa aatatgacca caccctcaga tttgaacatt
    43621 taccaactga ttgacaccca aaacggtcgg gtcactccgc gtatttatac cgacccggac
    43681 atttaccaac tggagcttga gcgtattttc ggtcgttgct ggctatttct cgcccacgaa
    43741 agccagatcc caaaacccgg tgatttcttt aacacctaca tgggagaaga tgcagttgtc
    43801 gtggtgcgcc agaaagacgg cagcatcaag gcgtttctca accaatgccg ccaccgggcc
    43861 atgcgtgtga gttatgcaga ttgcggcaac actcgcgcct ttacctgccc gtatcacggc
    43921 tggtcttatg gcattaacgg cgagttgatc gatgtaccgc tggaacctcg cgcctaccca
    43981 caagggttgt gtaaatccca ctgggggcta aacgaagttc cttgtgtgga gagttataaa
    44041 gggctgattt ttggcaactg ggataccagc gcaccgggcc tgcgtgatta cctgggtgac
    44101 attgcctggt atctggatgg catgctggat cgtcgcgaag gcggcaccga aattgtcggc
    44161 ggcgtacaaa agtgggtgat caactgcaac tggaaattcc cggcagagca gttcgccagt
    44221 gaccagtatc atgctctgtt cagccatgct tctgccgttc aggtattagg ggcgaaagat
    44281 gatggcagcg ataagcgcct cggtgatgga caaaccgccc gcccggtgtg ggaaaccgcc
    44341 aaagatgccc tgcaatttgg tcaggatggt cacggcagcg gcttcttctt tactgaaaaa
    44401 ccagatgcta atgtctgggt cgatggcgca gtttccagct attaccgcga aacctatgcc
    44461 gaagctgaac aacgtttagg tgaagttcgc gccctgcgcc tggcgggtca taacaatatt
    44521 ttcccgacac tttcctggct caacggcact gccacactcc gcgtctggca tccgcgcggc
    44581 cctgatcaag ttgaagtgtg ggcgttctgt attactgaca aagccgcctc cgatgaagtt
    44641 aaagccgctt ttgaaaacag cgccactcgt gcttttggtc ctgctggttt tctcgagcag
    44701 gatgactcgg agaactggtg tgaaatccag aaattgctta aaggccaccg cgcccgcaac
    44761 agcaaactgt gtctggaaat ggggcttggt caggaaaagc gccgcgacga cggcattcct
    44821 ggcattacta actatatctt ttcagaaact gctgctcgtg gaatgtacca acgctgggcc
    44881 gatcttctga gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag
    44941 gaggtgatga aatgagtgcg caagtttcac tagagttaca tcaccgcatt agccagtttc
    45001 tctttcacga agccagctta ctggacgact ggaaatttcg tgactggctg gcacagctcg
    45061 acgaagagat ttgttacacc atgcgcacca cggtgaacgc gcaaacgcgc gaccgccgca
    45121 aaggtgttca gccgccgaca acctggattt ttaatgacac caaagaccag ctggagcggc
    45181 gaatcgcccg tctggaaacg ggcatggcat gggcagaaga gccgccgtca cgcacccgcc
    45241 acttaatcag caactgccag ataagcgaaa ccgacatccc aaacgtattt gctgttcggg
    45301 tgaattatct gctttatcgg gcacaaaaag agcgcgatga aacgttctat gtcgggactc
    45361 gttttgacaa agttcgccgt ctggaagatg acaactggcg cttgctggaa cgggatatcg
    45421 tcctggatca agcggtaatc acttcccata acctgagtgt actgttctga tgaatcgaat
    45481 ttatgcgtgt cccgttgcgg atgtgccgga gggtgaggct ctccggatcg atacctcgcc
    45541 cgtcatcgcc ttgttcaacg ttggcggcga gttttatgcc attaacgatc gttgcagcca
    45601 tggtaatgcg tcgatgtcag aagggtatct ggaagatgac gccacggtgg agtgcccgct
    45661 acacgccgcc agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc
    45721 gctcaccact tatccagtac acgttgaagg tggtgacatt ttcatcgact taccggaggc
    45781 gcagccatga gcgatctgca taacgagtcc atttttatta ccggcggcgg atcgggatta
    45841 gggctggcgc tggtcgagcg atttatcgaa gaaggcgcgc aggttgccac gctggaactg
    45901 tcggcggcga aagtcgccag tctgcgtcag cgatttggtg aacatattct ggcggtggaa
    45961 ggcaacgtga cctgttatgc cgattatcaa cgcgcggtcg atcagatcct gactcgttcc
    46021 ggcaagctgg attgttttat cggcaatgcg ggcatctggg atcacaatgc ctcactggtt
    46081 aatactcccg cagagacgct cgaaaccggc ttccacgagc tgtttaacgt caacgtactc
    46141 ggttacctgc tgggtgcaaa agcctgcgct ccggcgttaa tcgccggtga aggcagcatg
    46201 attttcacac tatcaaatgc cgcctggtat cccggcggcg gtggcccgct gtacaccgcc
    46261 agtaaacatg ccgcaaccgg gcttattcgc caactggctt atgaactggc tccgaaagta
    46321 cgggtgaatg gcgtcggccc ttgtggtatg gccagcgacc tgcgcggccc acaggcgctc
    46381 gggcaaagtg aaacctcaat aatgcagtct ctgacgccgg agaaaattgc cgccatttta
    46441 ccgctgcaat ttttcccgca accggctgat tttacgggtc cgtatgtgat gttggcatcg
    46501 cggcgcaata atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg tttagcaatt
    46561 cgcggcattc gccacgtagc ggctgggttg gatctttaag gaagtacgat gaaagaaaaa
    46621 acgatcatta ttgtcggggg cgggcaagcg gcggcaatgg ctgcggcctc gctacgccag
    46681 caagggttca ccggtgagct gcatctgttt tccgatgagc aacatcttcc ttatgaacgc
    46741 cctccgctct cgaaatccat gttgctggaa gattccccac agttgcagtc tgtgttaccc
    46801 gctcactggt ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat caaaacattg
    46861 ggccgcgaca cacgagagtt agtgttagcc aacggcgaaa gctggcactg ggatcagctt
    46921 tttatagcaa ccggcgcggc agctcgaccg ctgccgttgc ttgatgcact gggagaacgc
    46981 tgctttaccc tgcgccatgc cggtgatgcc gccagactgc gagaagttct gcagcccgaa
    47041 cggtcagtcg tgattgtcgg tgccggaact attggtctgg aactggctgc cagcgccacg
    47101 cagcgcagat gtaaggtgac agtgattgaa ctggcggcaa ccgtcatggg ccgtaatgca
    47161 ccaccgcccg tgcaacgcta tcttttacag cgccatcagc aggctggtgt gcgcattctg
    47221 ctcaataatg ccattgaaca tgtggtcgat ggtgaaaaag tagaactgac gctgcaaagt
    47281 ggcgagacgc ttcaggccga tgtggtgatt tacggtattg gtatcagcgc caacgaccaa
    47341 ctggctcgcg aggccaacct tgatactacc aatggcattg tcattgatga ggcttgccgc
    47401 acctgcgatc ccgcgatctt tgccggtggc gatgtggcaa tcactcgtct tgataatggt
    47461 gcactacacc gctgcgaaag ctgggaaaac gccaataacc atgcgcaaat tgccgctgcc
    47521 gcaatgttag ggctaccgct tccgctactg ccgccgccgt ggttctggag tgatcagtac
    47581 agtgataact tacagtttat tggcgatatg cgtggcgatg actggctttg tcgtggcaac
    47641 ccggaaaccc agaaagcgat ttggtttaat ctgcaaaacg gcgtgcttat cggtgcggta
    47701 acgctgaatc agggacgtga gattcgctca atccgcaaat ggatccagag cggcaaaacg
    47761 tttgatgcga aacagctgac agatgagaac atcgcgctta aatcactgta accaggataa
    47821 ttagcgaata tctcaacgcc tggggcgtgg cgaggtacca gagtgtgtat tacgtttaaa
    47881 tcacattatc ttgcaaaggg attggttatg aactcactac gttattttga ttttggtgct
    47941 gcccgccccg ttttgttatt aattgcccgt atcgccgtgg tcctaatttt cattattttt
    48001 ggttttccca aaatgatggg ctttgacggt acggtccaat atatggcctc gctgggcgca
    48061 ccgatgccga tgctggcagc gattattgcg gtagttatgg aagtgcccgc cgcgatatta
    48121 atcgtgcttg gctttttcac ccgtccgctg gcggtactgt ttattttcta cacgctgggt
    48181 acggcggtga ttggtcacca ttactgggat atgaccggcg atgccgtcgg gccaaatatg
    48241 attaatttct ggaagaatgt cagtatcgct ggcgcgttct tgctattggc aattaccggg
    48301 ccgggggcaa tttctctcga tcgccgttag gaaagatgcc ggatgcggcg tgaacgcctt
    48361 atccggcatt ttataaatta cagccactct acccgcaacg acatctctga ggttgttgat
    48421 tcccccggcg caagcgcaat gaggtcaccc ccttccgggc gatgatggtc atccggcgca
    48481 tggctcatcg gctccagaca gaagaaatca aacgcatatc ctttatcaaa cgccgggtcg
    48541 gaaacaaaga tgaaataaca cggtgcaggt ggcgtcgttt ctatgatgat ggcataaccc
    48601 tcctgcggct gctcgatgcg ggcctgacca ttccatccgg caaaaccatt gttcacccac
    48661 tggcgcggca acggcgctag ctggttaaaa tccagttctt gcggtagctg ctcgcaaaac
    48721 tcacccgcca gccactgctc ccgctccagc cagtaaccgc tcgcctgtgc ctgaatccgc
    48781 gtttgcggcg acaacggaaa ataaggatgc cagccggtac caaatggcag cgtctctgcc
    48841 ccttgattgg tgacagagag cgtcaccgtc agcgtatccg ccgttaaatg aaacgcctga
    48901 cttacccgat agtgatagac accgctgcga tgttcataca ccagacacaa actatcatcg
    48961 ctgtgcgaga cacattgcca ctcgcccagc cagccatcgc cgtgtagata gtgcgcatcc
    49021 cactcaacat tcggttgcag ttgatactca cgcccctgcc agacaaaacg gttgccgctc
    49081 acccggtttg caaatggcac cagcggaaaa caagaggcat cggttgccac accgcttttt
    49141 ttaccaggac gtaagagcgg cgtcgtatcg cgccagaagc cttcgattac gccgccctgg
    49201 tcagaaacgc ccagctttag cgacccgtgg gataaggtat agatggtcat ccgctaactc
    49261 cttaatccgg gaagttaatc acaactttcc cgcatttgcc gctcgccatc agcgcatagg
    49321 catcacctgc ctgttccagc gagaagcgat gggtaatggc gttacgcggc cacagcttcc
    49381 agtcagtcaa atcgtgggcg catttttcca tatggaacag actggtcacc caggagccaa
    49441 taatccgccg ttgatggtgc atcagatcgg cgctgacctc gaattccact tttccggttt
    49501 caccaatgta aaccacccgc ccccagtcag cggtggattg cagtgccagt aagcgacctg
    49561 cggcattacc ggaacaatca agcgcaacat ccgcgccgcc gtgagtgagt tcggcgatta
    49621 tctgcggcag accttcagtg gtcgctaaat agccgtgatc catcactcct aactgttttg
    49681 ccatcgccag acgttccggc agcatatcaa cgccgataat ccgttttgca ccgcgacctt
    49741 tcgccagcat catccccatc atgccgactg gccccagacc gaccaccagc acgttatcac
    49801 tgccggaaac ttcgccgcgc aaaatccctt cataggccgt gccaacgccg caactgataa
    49861 acgcaccatc ttcgtagctc agcgcatccg gcaggaggat cagatctttt tcttccgcca
    49921 gcaagtattc ggcatgaccg ccgtcacgct gccagccgta agccgctttt ccttcgccag
    49981 tacaagaaat aggaaaaccg cgacggcagt tcgggcaaaa accacagcca gaaatgtgat
    50041 acaccagcac gcggtcgccc tctttaaaat ggcggcagcc ttgccccatc gccacaatct
    50101 gcccgcacgg ttcatgaccg ttgataaatc cctagtacaa cggtttatcg gatgccgccg
    50161 ccgtagcgcg gtgctggtgg tagatatagt ggacatcgct tccgcaaatt ccggaggatt
    50221 tcattttgat cagtacctgg ttaatccccg gcgtcggcac tgcaacttcc cgcagatcga
    50281 cggtcgaatt tcctggtaaa taagctgcca gcatcgtttt cataaatccc tcattaacaa
    50341 tacgattaat tttcatccct gccgcacccg cgccaggggc gttaaggttt agcgtttcgc
    50401 tttactgcta cgctgggtca gcaagatatt cgccagcacc gccaccacga tgatgacgcc
    50461 gcgcaccacc tgctggaaaa aggagttaat cccgagcagc accagaccgt taccgattag
    50521 cgtaatcacc agtacgccaa gcagcgtacc gaacaacgag ccgcgaccac cggaaagcgc
    50581 cgtaccgccg accacgaccg cggcgatgac gtcaaactcc agaccgtttg cggcacctgc
    50641 gttaccggaa ccgaggcgcg ccgccaacaa aatgccggtc accgccgcta ataatcccga
    50701 aagggtaaag ataagaatgc gtacccgacg aacgttaata ccgcacaact gcgccgccgt
    50761 ggcattaccg ccaacggcaa aaaccgagcg cccgaaggcg gttttgcggc taatgaacac
    50821 aaacagcgca aacagcacca tcatgatcag cgcggatacc ggcacaccga gaaattgtcc
    50881 gcccagccag tccagcacct cgttttcgtc aatcggcact ggcagagcgt tggtcataaa
    50941 caagcccatt ccgcgcaggg cgctccacag ccccagcgtg gccacgaaac ttggcacgtt
    51001 aaacacgcca cgcagcaccc cggcgagcgt tcccatcagc gcgcctaaca gcaacaccag
    51061 cagacaagca atcgccaacg gaacttcaaa ttgcagcaaa aatgccaggc ataccgagac
    51121 aaaagccacc atcggcccaa cgctgacatc aatttcaccg gagataataa tcagcgtcat
    51181 cgcccaggcg gcaatcccaa tggtggcggc atcgcgcagc acgttcatct ggttattcaa
    51241 tgagataaag ccaggcgcgt tcagggagaa gaccagataa agaatggcaa tcaccaccag
    51301 caaaccgatc tcattaatat ggtgactgac aaattgtttg agcgagacgc tcttgccctg
    51361 cggcaatggt aatgacgaag cagacatctt caggttcctc ggtaaaattc atcagtgcac
    51421 agacagaatg gcggacatca gctcatccac attgaccgga gcgtgaaact cctgcgagaa
    51481 cgtgccgtgc tgtaataaca ggatgcggtc acacaccaac ggcaactcct ccacttcact
    51541 ggagataaac accacgcttt ttccttcggc agccagctca cggacaatac ggtaaatctg
    51601 ctgtttggct tcgatatcga cgccgcgcgt tggctcgtcg agcaacaaaa tctggctggc
    51661 ggcatagacc caacgaccga tcactacttt ttgctgattg ccgccagaaa gcgtgccgat
    51721 gggcgtttcg ctactggcgg ccttgaccgt catccgctgc atcacttctt cggtcaggcg
    51781 gcggatggtg gaccattgca gcactccgtt ggcgctgatt ttttgccgat tggtcagcac
    51841 tgtattttcg tcaacgccca accagggaat gatccccgct tctttgcggt tttctggcgt
    51901 atagccaatg ccgcgtttca gcatgtcgcc gtaatcgggg cgcgtgattt tctcgccgtt
    51961 gataacaatt tcgccctgtt catacgtctc cagcccaaca atcgccttca gcaattcact
    52021 gcgccctgcc cccagcagac cagcaatgcc gagcacttcg ccacgacgta gcgtaaagct
    52081 gatatcctcc agcttgggct tatggcgtaa cgcacggact tccagcacgg cctgatccac
    52141 aatttcctga ggtgcaaccg gcgcaatatc aacgtgatcg cgccctagca ttagcgacac
    52201 aatatgatgc gtggaagtgt tttcgagcat cacatcgcca gccacctgcc cgtcgcgcat
    52261 aacggtggca ctggaggcaa tgcggcgaat ttcttccatc cggtggctga cataaatcac
    52321 cgccacgccc agtgctgaca tctttttcac cgctctgatc accagttcaa cttccgcact
    52381 cgcaagcgaa ctggtaggtt catcaagaat gaccacgcgc ggctcgccct tcatcacccg
    52441 cgcaatttcc accagctgct tatgcgccgg gcttagtgtt gaaacaagtt gttcaggact
    52501 aacgtcaacg cccagcgcct gtaagcaacg ttgggcatcc tgcgccattt gcaggtaatc
    52561 aatcatgccg ttgcggcggg gccactgacc gaggcagagg ttttccgcca ccgtcagccc
    52621 ttccaccaga cttaactcct gataaaccgc gcgtaccccc agttcagcgg cacggcgagt
    52681 cagcgtagct tcgtcacctt ccagtcgcgt ctcgccaatc cagatttcac cgctatccgg
    52741 acgttcactg ccggtaagca ttcgaatgag ggtcgatttg cccgcgccgt ttttgcctaa
    52801 cagcgcacga acctcgcctt tattgagcgt gaagttaacg ttatccaacg caacgacgcc
    52861 gggataacgc ttatttcctg ccaccacttt tgctaccggg actgcctctg ttgccgtgaa
    52921 catagggacc tctgcgaatc agcgattagg gcagaccatc aacgtgcgtt gccagccact
    52981 gtttaccgtc ttccgttttg gtatagagat cgataggcac ctgaatcact ttttcaccgt
    53041 cggcttgttt attgataacc ttcaatgttt gcgcgaaaac agcattgccc attttcttac
    53101 cggaaatatc cactaccgct ttcagcacct gattgttttc cagctcctga gcaatttcgg
    53161 ttgtcatatc cgaaccgaaa acagcaattt ttccggcctg attttgatta cgtaccgctt
    53221 ttaccgcacc gagtgtcgca ccgcccgatt cccccataat ggcgttgaga tccggcgtgg
    53281 agataatcag tttttcacca acggaaatcg ctttatctaa aacagtccct tcctgattag
    53341 cgacaatttg cgcgccggga acgcgggatt ttaatacttc ttcaaatcct ttacgtcgct
    53401 gcacacaaac ttcaaaggct tcgcaattga tgacggcaat tttcggctgg tcaattttat
    53461 tggcaataaa ataatcggcg gcagcgttac ccagtttttt accaaattcc agcgggtcac
    53521 ccaccagata cgccgagaca tatttatcga cccccttttg attaatacag gtgttgtagc
    53581 aaatcaccgg aatacccgct tcactggcgc gacgaacggt acggctactg ccattttcag
    53641 acactgccga taaaataata gcatcgacat tacgcgccac gagggtatca acaaaggtac
    53701 tttctttcga aatatcaccc tgggcgttag tttcaattaa ctgtacctgt actgaagaat
    53761 cttttgccgc atcctgaact ccctgacgca ctccagcgta atatccctga gtatcaaggt
    53821 atattgcgcc aatggtcatt tctttatccg cagctctggc aaatagtgcg ctacctaata
    53881 gcgtagccat taataataga ttacgggttg ttctcatttt tttaggcata gagcctcctg
    53941 tagggttttt attaacaacg gcttattcta attattttgt gatgagcggc agcgcgtgcc
    54001 gctcaaatat tacagttaat agggtttagt gaacgtagtt aaaaataaaa ggcatcacgg
    54061 tggtgaataa tgccgctttc ggccaggccg gattaatcgc cgtcaattca gcgcgagcag
    54121 attcgtattg cgcaatctcg cctaatcccg cggccgccag catcgccacc atcaggcatt
    54181 tttctttatg ctgctgttgt aaatcgccat acagcgacaa caagtcaggc tgcgagacgg
    54241 caaagaaatc cgcttcgata ctggttttcg ccatctcttg cgcccactgt ttcatttcac
    54301 taaacagttg ctgtgcggtg tgttgttcgc ccagtaatcg cagcgccatt ccttgccaga
    54361 agaggtaatc aaccggctga tcgttgtaat agctgtggat gttaatggtg cgatcgccgg
    54421 tcgccgccag atgtaaacag cgcgtcgctt cagtttcatc gccctgggct ttggcgcata
    54481 tcgcctgcca gaaccagatg tcgttatcag tttgccccgg taaacggcct tcgcttaaat
    54541 tctccggata atgcagcgcg gcatgaagca gttcgctggc ctgctgcggc tctctggcat
    54601 caagatgctg ccaggcgcgt aataactggt tgaggataaa ctgactggtg accttccctt
    54661 ccccgccttc ccacgggtgg aatttgcgcg tggcgagaat gtccgccgct ttgtctgcct
    54721 gccccgtgag atgccacaaa ttgagcagtt ctgcggtcat gtcgtcgcgt ttcagcgcaa
    54781 tttccagatt attttccaga cgcgccagtc gtttctccgg tgtggttcca cttagcttat
    54841 caagcaaatc ccgttcgaaa agcagacgtg catcctgcgg cgcaagctga taagcattat
    54901 caagataacg cgcggccagc tcataatcgt gttgcttatt ccacgcatgg atcgctaacc
    54961 cgcgccagcc gtcggcaaac tccggcgaca tctctacgca acgttgccat aaggcaatgg
    55021 cttcgttgta gctacgtttg ttgtagtaga agcaggccag taaatggcga gcaaaccagc
    55081 actcttcaat actctccagc gccgccactt cttccagcgt attcgggaaa cggacaaact
    55141 gcgggaagac atcaatggct tttgcgacca gttcgccacg ttcggctttc ggcagcaagc
    55201 tggcttgcag gtaaagcggc agcgtgcgct ggcagtccag tgcattcagc atctctgccg
    55261 cgagggtggg cattccccag ttaatcagtt gcccggcggt catcagggcg ttaacgtcgc
    55321 gtccctgaca cagaccgcgc cactgcgcga gagctgattc gctacgacca tcgaaccagt
    55381 tcaggcacca cagagtggcg ttcagcggat aatcgcgcag cagtttctcg cgctgcaaac
    55441 gcgcgttgtc ctgacgacca cttaacacca gcagcagatt atgcaggcaa agcacttcct
    55501 gattgattgg gcaggcgcga agactttgtt ggcaaaaatc cagacccgcg tcgaagttac
    55561 cattacgcgc cgccagtcgt gccagaccat aatagcctcc ggctttactg ttgccgctcc
    55621 agaccgcacg ccagaaatcc tcttcggctt gttgatattg tccctgacgt tcgtaagcac
    55681 tggcgcgaat caaactcgcc tgtccgcact gcggattttt gttcagcgca tgtgcgcgtt
    55741 tcagagcctg agtggcatac gccaccgctt gcgggaaatc tgcgcggtta tactccagca
    55801 tcgccagcgc cagattacag cggtaatcca gcggatccag cgccacgccg cgcaggtagt
    55861 aatcgaacgg tgaacggctg gcgtgatgat attgctccag atgctgaccg ataaaccagg
    55921 cttcatctgt actggtaatg tcttgtgctg acagtggcgc tttggcgacg tccggcaacg
    55981 gcaactcttg cggctgatgt tcctgataac tcagtacaat attgccgtcg gcatcagaga
    56041 gctcaatggt cagtctttca ggattgatac cgtgtaacac gccctggatg gcggtcgctg
    56101 gtgtcagggc cacggcatca tcgagcaacg cgtcgcattt gccgatttcg cggatcgcca
    56161 ggcggtatcc gttcaacgga gagatggcat acagccccca ctcaatcccc cgctcactac
    56221 gctggagttt gattaccgca tcgcgggagg cgttttgcac catgcccaga gaatggtaag
    56281 gcaggaaaaa ctgctcaaaa cgcttctctt cgtaagcatc aagccgggta aaatcaggct
    56341 ggttatcggc aaaaataccg gtcatcagtt cgatatacgg gccgttatta tcagttaggc
    56401 tcttatccca cgcctggcca aattcactgt gtccccagct ccactgcttt ttacctggcg
    56461 caatatggtg gttggcaacg tgtagcaaac cgccatcttc atcgtgacac cacgcgccaa
    56521 caaaatcgta ctgtgatttt tcagccatat atgaggttgg aacgggcaca tttttatagc
    56581 gagaaatgtc cactccagcg gagtagtcca ctttgtagta agtgccggtg gcgataggga
    56641 aagcggagac ggcccgtttg ccgtgatcaa acaccgccgt tacatccggc ggaaagacgc
    56701 tctgatgccc ttcaccccct ttcactgccg ggttggccca ccacaagaaa tgacgcggcg
    56761 tggcgttgcc gttatagacg cggctggcga tttccagcgc cgcccggtca gggcgcaggg
    56821 tgaaacctgt catcacctgt aaaccatgca tcggctccgt ttcgcctacc cacaccgtct
    56881 gtgcgccgtc ttcatgggct tcgagggtga aatcaacggg cataaaggtg gtcgggcgat
    56941 ggtgttgcgg ccagttaaac tcaatcccgc cggagatcca cggtcccagc agccccacca
    57001 gcgcaggttt aatgacttca ttgtgataga caaaatcgcg ctgtttcact ttatcccatg
    57061 cgcgatgcac ccgaccgccc aattccggca ggatcatcac tttgatgtag tcgttttcca
    57121 gccacaccgc ctgccaggat ttcagggttt tctgctcgct cagcgtatcg gtcacgccgt
    57181 agggataaac cgcgccggac gatccctgat aaacgcgatt ttccaggaac atgggatgta
    57241 tatcctgcgg cccggtttca taggtcggga tctcaacgcg ctcttgccac acttttactg
    57301 gagtcatggt gccctcaata ttaacaagac atactgaatt aaaagatttg tggcagtgta
    57361 ttgaacaatc tggcaatgtt ttcgcggaat aatcacgcaa ttaactaaac aaggtttagt
    57421 gaagatgaga gcctgcatta acaatcaaca gattcgccac cataacaaat gcgtgattct
    57481 ggaactgctg taccggcaaa agcgcgccaa taaatcaacg ctggcccggc tggcgcaaat
    57541 ttcgattccg gcagtcagta atattttgca ggaactggaa agcgaaaaac gggtggtgaa
    57601 tatcgacgat gaaagccaga cgcgcgggca tagtagcggt acatggctga ttgcgccgga
    57661 aggtgactgg acgctgtgtc tgaacgtgac gcccaccagt attgagtgtc aggttgctaa
    57721 tgcttgttta agtccgaaag gtgaatttga gtatttacag attgatgcac cgacgccgca
    57781 ggcgctgctg tccgaaatcg aaaaatgctg gcatcgccac cgtaaattgt ggccggaccg
    57841 caccatcaat ctggcgctgg caatccacgg tcaggttgat ccagtgactg gagtgtcgca
    57901 aaccatgccg caagcgccgt ggacaacgcc ggttgaggtg aagtatctgc tggaagagaa
    57961 gctgggcatt cgggtgatgg tcgataatga ctgcgtgatg ctggcgctgg cggaaaaatg
    58021 gcaaaataat tcgcaggaac gggatttctg cgtgatcaac gttgattacg gcattggctc
    58081 gtcgttcgtg attaacgagc aaatttatcg cggcagtttg tatggtagcg gacagattgg
    58141 tcacaccatc gttaatccgg atggcgtcgt ctgcgactgt ggacgttacg gctgcctgga
    58201 aaccgtcgcc tcgttaagtg cattaaaaaa acaggcgcgg gtatggctaa aatcacaacc
    58261 ggttaatact cagcttgatc ctgaaaaact gactacagcg cagttaatcg ctgcctggca
    58321 aagtggagaa ccgtggatca ccagctgggt tgatcgctct gccaatgcca ttggtttgag
    58381 tctgtataac ttcctcaaca tcctcaatat taatcagatt tggttgtacg gtcgcagttg
    58441 tgcctttggt gagcactggc ttaatactat tattcgccag acaggattta acccgttcga
    58501 ccgcgacgaa ggaccgagcg tgaaagcgac gcaaattggc ttcgggcaat taagccgcgc
    58561 acaacaggtg ctgggaattg gctatttgta tgttgagacg cagttacgac agatttgatg
    58621 gcgcgataac gtagaaaggc ttcccgaagg aagccttttt caacagatta accgtttcgc
    58681 ttatgcgtaa accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat
    58741 aacggcttca tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc
    58801 ttctacttct ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa taccggaggt
    58861 cacaaacggg ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg
    58921 gcccagagcg gcgtctgctt ctttaccggt caggttttta tcgaccagat caaccaggaa
    58981 cagatggtta tcagtaccgc cggaaaccac tttgtagccg cgttccagga acacttctac
    59041 catcgcttta gcgtttttag cgacctgctg ctggtaagtt ttgaactcag gctccatcgc
    59101 ttctttcaga gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc
    59161 agggaaaacg gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat
    59221 cagaccgccg cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg
    59281 aggaaccggg ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac
    59341 gaacaggtaa gcgccgatgc tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc
    59401 ggaatatgca gagaagccac cgataatcat tttcggtttg tgttctttgg cttgtttttc
    59461 cagatcggcg tagtcgatat gaccggtagc atcaataccg taaggaacaa tgttgtacag
    59521 tttaccggag aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat
    59581 acccagaacg gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga
    59641 gccggagtgc ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat
    59701 cgccagttgt tcaacgatat caacatactc gcaaccaccg tagtagcgtt tgcccggata
    59761 accttcagca tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt
    59821 gtagttttcg gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat
    59881 agcctgccac agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat
    59941 ctcctgactc agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg
    60001 cgaacagtat aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac
    60061 gattaccttc aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt
    60121 tcttttcagg tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa
    60181 agaaccattt acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag
    60241 atgcaaaaaa aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc
    60301 atccctttac tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt
    60361 actcataacc cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa
    60421 cgtgaagccc tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg
    60481 ctgctgccag cggtagaaaa aatcgcgcag aagcacacca gtttccagat caaaccggaa
    60541 cagtacaaca tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg
    60601 caggaagtgc tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat
    60661 cgcgaggcgg aaatctataa cgaaaacgcc agcaaagccg gtggctggga aggtactcgc
    60721 gatttccgca ttgtggctaa aacacctcgc agcgcgctta tcaccagctt cgaactggag
    60781 ccggtcgacg gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg
    60841 aagccggaag gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat
    60901 ggcaaaggct atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg
    60961 cacaatcacg ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt
    61021 atggctgtcg cagatgacac accagtgacg ttaatctctg ccggtgttgg tcaaacgcca
    61081 atgctggcaa tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc
    61141 catgcggcag aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag
    61201 tcactgccgc gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct
    61261 aaaggtcagt ttgatagcga aggtctgatg gatttgagca aactggaagg tgcgttcagc
    61321 gatccgacaa tgcagttcta tctctgcggc ccggttggct tcatgcagtt tgccgcgaaa
    61381 cagttagtgg atctgggcgt gaagcaggaa aacattcatt acgaatgctt tggcccgcat
    61441 aaggtgctgt gatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa
    61501 attgccgcgt cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg
    61561 aagattttac cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag
    61621 gtatcgacaa tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc
    61681 atatactccg cgccgcggta cagctcggta tggcctttct ggcggccaaa acctttcacc
    61741 tcagtgaccg tcatgccggt aatgccgact tcagccagtg cttcgcggac atcgtccagc
    61801 ttgaagggtt taataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt
    61861 cttttgatct gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc
    61921 agctcgtgtc gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc
    61981 gcgtgggtga cgttgccttt ggtaatttgc agcagcttac gcaaatagtt gagttcaaac
    62041 tgattacgtg cctcaacaaa ggttggcagc gccgtatttt caccctccag cgcctgctct
    62101 actaatgcat cgctaatcac cggagatgag gtcagcgcca cgcactgttc aatcacgttg
    62161 accaactggc gcacattacc cggccagctc gcggtcatca ggcgtttcat cgcatcggtg
    62221 gaaaacgcgc ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc
    62281 agtagcggaa tgtcttctgt gcgctccgcc agtgccggaa ttttcaggct gacaacgttg
    62341 aggcggtaat agagatcttc gcggaattcc ccgcgcgcca tcgcttttgg cagatcacgg
    62401 tgagtggcag aaataatccg cacattgata tcaatatcgc ggttactgcc cagcgggcgc
    62461 actttacgct cctgcaacac gcgcaacagt ttgacctgca acggcgcagg catatcgcca
    62521 atctcatcga gaaatagcgt tccgccttcc gccgcctgga ataaaccttc gcgattgctg
    62581 acagcgccag taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctca
    62641 ggcaatgcac cacagttaat agcaataaat ggtttgctat tgcgcgggct ggcgttgtgg
    62701 atagcctggg cgaaaatctc tttcccggtg ccgctctggc cattaatcaa aacgctgacg
    62761 tctgattgcg ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg
    62821 acaatcgcct cgcgccagcg ttcgtcggtg gctggcgcgg attgttccag cgcatcatca
    62881 attgcctgat atagcgcgtc tttatcaaca ggcttggtga ggaaactaaa aacgccctgc
    62941 tgtgttgcag caacggcatc gggaatagaa ccatgcgcgg taagaataat taccggcatt
    63001 cccggctgca ctttctggat ttcagcaaac agctgcatac cgtccatttc atccatccgc
    63061 aggtcgctga tgactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca
    63121 ctttccgccg tgaccacgct gtagccttcg ctggtcaggc gcaggccaag cagtttcagc
    63181 aatcccggat cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac
    63241 ctcatgggtg gatggcgcgg gcttttcact ctcgtgtggc gtatccggcg agaaatttcc
    63301 agccggtttg cgggtcgaga gctggcgttc aatatcagtc aggttttcca gcttgcgggt
    63361 ggtgagttcc agttgctgct gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct
    63421 gtcgctggac tgctggagtt tactgtagcg ttggcgctct tccgccagtt gcagttgcag
    63481 cgcctgaccg tcgcgccaga gttgatacag tgggcgaacc tgtgccggga tctcggtact
    63541 taacgcttca atacgcgcga ccagctggcg gcgctcatat ggcgtaattt tggcgtcggc
    63601 gagcaaaatc ccttgtttaa aggtattttg ccagctgccg tcgtcatatt ggcgagcttg
    63661 ctgacgcgac tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag
    63721 cggattggtt tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag
    63781 atagtcagcc agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt
    63841 attgtgattc tgcacgcaac ctagcaatgc cagacatggc agccctgcca gccacagccg
    63901 tcggggcaat aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag
    63961 tgggcgtaat tgttgcagac agtttggaca tggacagcgc ggagaaaccg gagcgtacat
    64021 gtcgtacgtg aggatttcga gcactgcccg gggccgaact gacaaataaa atagcctgat
    64081 gggataggct gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa
    64141 acgtcttgcc cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc
    64201 ctggcaatgc ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc
    64261 tgaaaaaagg gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg
    64321 atgacatcaa tataaacccg cgcaccatgt aaactgctgc gaaggcaaat gttaccggat
    64381 tcagccccgt agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt
    64441 ggctccgcca ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt
    64501 gcgggcaggc tatgagcaga aactactgtc tccaccagcg gtgctaactc aacattatct
    64561 agttccaccg cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt
    64621 tgcaaattgc ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc
    64681 ggcccgacaa cctgatcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt
    64741 tttaattcat gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg
    64801 cgctcactta accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac
    64861 gaaacgctat tgcccagaga acgcccttcc cccaaccgat tgatcatccg ttcaatattt
    64921 ttcaccggcc cgataatcat ccgcgtgaaa agcagtacca ttaccagact caccagaaat
    64981 agcaccagcg attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc
    65041 tgcccacgag agaacactac tgtgcgcgtg gcctgtacca tttcggtatt ggcactggca
    65101 aaggcttcca gacgcgcggc agcagcagca tcgggaccac tgttgttgca ctgaagttgg
    65161 gccagattgt tcaagtcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct
    65221 gcgtgggcat cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc
    65281 gtagggtcat ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg
    65341 gtcattgctt cgctgcgccg ggcatcgata agcgtggtgc ggttgaccag cgccgcctgg
    65401 tcgctaagcg cattcaggct ttgccaggct tgccatgcca gcaccaacag gggcagcaga
    65461 atcagcaaaa atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt
    65521 ttcaaggtgt tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga
    65581 tacaacaaag ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg
    65641 tttcgcccga tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt
    65701 tgtgcgtcat tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg
    65761 agccactgat tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac
    65821 tgattatacg ttgctttgag gggtttatgg cttccccgct atttgaatgt aaatcagcca
    65881 ctgtgtcgct aaaaagagac aacttaagaa aaacttatta gattacaata aaatcaatca
    65941 tttaagtgtc gccagaaagc gacacggtaa accacccatt gtcgtgattt acagacacaa
    66001 aaaagctccc gaagttggga gcttataata gcggttgtat ttaatgccgg atgcggcgtg
    66061 aatgcctcat ccgggctgca aaaccaatga gtttgacggc ttaccccaac tgcttacgtg
    66121 cattgcggaa aatgcgcatc catgggccat cctcgcccca gttttcagga tgccaggagt
    66181 tgctgacagt acggaaaaca cgttccgggt gcggcatcat aatggtgact cgaccgcttt
    66241 cagtcgtgac cgccgtaata ccgttcggag aaccgttcgg gttagccggg taggtttcag
    66301 tgactttgcc gaagttatcg acatagcgca gtgccaccag ccctttgctt tccagtgccg
    66361 ccagatgcgc cgcatcacgc acttccacgc gcccttcacc atgagagaca gcaatcggca
    66421 tttgcgagcc caccatcccc tgcaacagca gcgacgggct ttgggtcact tcaaccaagc
    66481 tgaaacgcgc ttcaaagcga tcggaggtat tgcgcacaaa acgtggccac aactcactac
    66541 ctgggatcag ttcacgcaga ttagacatca tctggcaacc gttacatacc cccagcgcca
    66601 gcgtttgcgg acggtggaag aaggttgcaa actcatcgcg tacacggtca ttgaacagga
    66661 tagacttcgc ccaaccttca ccggcaccca gcacatcacc gtaggagaaa ccaccgcacg
    66721 cgaccagggc gtggaaatcc tccaggcccg tgcgtccagc cagcaggtcg ctcatatgca
    66781 cgtcgatagc atcgaagcct gcacggtgga aagctgccgc catttcaaca tgcgagttca
    66841 cgccctgctc acgcagcaca gcaactttcg gacgtgcgcc agtggcaata tacggtgctg
    66901 ccacatcttc gttgatatca aacgacagtt tcacattcag gcccggatcg gcgtcgtttg
    66961 atttcgcctg atgctcctga tcggcacact ccgggttgtc acgcaggcgc tgcatctgcc
    67021 aggtagtttc tgcccaccag acacgcaacg tagtgcggct ttcgctgaat acagtctgcc
    67081 cgttggcggt aatcacaaaa cggtcaccgg aaaccgcctg cccaacataa tggacgcaat
    67141 cagcaagccc atgctgtgcc agtacggact cgaccgcttc acggtcagcg gcacgaacct
    67201 gaatgaccgc acccagttct tcgttaaaca acgccgccag gcgatcgtca cccagagtgg
    67261 cgatatccgc gtcaatgccg caatgaccag caaaggccat ttccgccagc gttaccagca
    67321 gaccgccatc agagcggtcg tgatacgcca gcaacttacg ctgtgcaacc agcgcctgaa
    67381 tcgcgtcata gaagcctttc agttgcgcga catcgcgtac atctgccggt ttgtcgccaa
    67441 gctgacgata aacctgcgcc agcgccgttg cgcccagcgc gttattgcct ttgcccaaat
    67501 caatcagcag cagtgcgtta tcttcggtag aaagctgcgg cgtgatggtg tgacgtacat
    67561 cttccacgcg ggcaaatgca gaaatcacca gcgacagcgg cgacgtcatt tcgcgctctt
    67621 cgttaccttc ctgccagcgg gttttcatcg acatagagtc tttacccacc gggatcgtca
    67681 ggcccagcgc cggacaaagc tcttcgccca cggctttaac ggcttcatac aggcccgcat
    67741 cttcaccagg gtggcctgcc gccgccatcc agttggcgga aagtttaatg cgtttgatat
    67801 cgccaatctg tgttgcggca atattggtta acgcttcacc gaccgccaga cgggcagagg
    67861 cggcgaaatc cagcagcgca accggcgcac gctcgccaat cgccatcgct tcaccgtagt
    67921 agctgtcgag gctggcggta gtgaccgcgc agttagcgac cggcacctgc cacggcccca
    67981 ccatctgatc gcgcgccacc atgccggtta cgctgcggtc accgatggtc accaggaaag
    68041 ttttctccgc cacggtcggc agatgcagta cgcgtttcac cgcgtctgca atggtgatcc
    68101 cttcacggac cagcgcatcg cccttcgctt tcagcgtttg tacatcgcgg gtcatcttcg
    68161 gcgttttacc aagcaggacg tccagcggca gatcgatcgg ctgattatca aaatgacgat
    68221 cgtgcagaga aagatgcagt tcttcggtcg cttcaccaat caccgcgtag ggtgcgcgct
    68281 cacgcttaca cagttcgtca aacagcggta attgatcggc agcaaccgcc agcacgtagc
    68341 gttcctggga ttcgttacac cagatttcca gcgggctcat gcccggttcg tcgcttagaa
    68401 tatcgcgcag ttcaaattta ccgccgcgcc cgccgtcgct caccagttcc ggcatcgcgt
    68461 tagaaagacc gccagcgccg acgtcgtgga taaacaggat tgggttggca tcaccaagct
    68521 gccagcaacg gtcgatcact tcctggcagc gacgctccat ctccgggttg tcgcgctgta
    68581 cggaagcaaa gtcgaggtcg gcatcagact gacctgacgc catggaagac gccgctccgc
    68641 cgccaagacc gatgttcatt gccgggccgc cgagaacgac cagtttcgca ccgacgttga
    68701 tctcgccttt ttgtacgtga tcggcgcgaa tgttgccgat cccgcccgcc agcatgatcg
    68761 gtttgtgata accgcgcagc tcttcgccgt tgtggctgtt cactttttct tcataagtac
    68821 ggaagtagcc attcagtgcc ggacgaccaa attcgttgtt aaacgccgcg ccgcccagcg
    68881 ggccttcggt catgatgtcc agcgcggtga caatgcgctc aggcttaccg aaatcttctt
    68941 cccacggctg ttcgaagcca ggaattcgca ggttggaaac ggagaaacca accaaacccg
    69001 ctttcggctt cgcgccgcgc ccggtggcac cttcatcgcg gatttcaccg ccggaaccgg
    69061 tcgccgcgcc cggccacgga gaaatcgccg tcgggtggtt gtgagtttcg actttcatca
    69121 gaatatgcgc cggttcctga tggaaatcgt agcggcccgt ttcgtggtca gcaaagtagc
    69181 ggcccacttc agaaccttcc attacggcgg cgttatcttt ataagcagag agaacgtgat
    69241 ctggcgtggt ttcgaaagta tttttgatca tcttgaacag cgatttcggc tgctgttcac
    69301 cgtcgataat ccagtcggca ttaaaaattt tgtggcggca gtgctcagag ttcgcctgag
    69361 cgaacatata cagctcgatg tcgttcgggt tacgaccaag ctttgtgaaa gcatcctgca
    69421 gatagtcaat ttcatcttcc gccagagcca ggccaagacg caggttagcg tcgatcagcg
    69481 cctgacggcc ctgccccagc aaatcaacgc tggtaaccgg agtcggttga tggtgggcaa
    69541 acaactgctc tgcatcatct aaagcaaaaa agacagtttc catcatgcgg tcgtgcagtt
    69601 cagcggtaac ctgctgccat tgttcattgg tcagcgtacc ggcttctata tagtaagcaa
    69661 cgccgcgctc aaggcggttt acctgttgta gcccgcagtt atgagcaata tcggtcgctt
    69721 tcgaagacca gggagagatg gtgccaggac gcggggtcac cagcaggagt ttgccttgcg
    69781 gggcgtggct ggcgagtgcc gggccatatt tcagcaggcg ttcaagttgt gcgtgctcat
    69841 catcgtttaa cggcgcattg aggtcagcaa aatggacata ctcggcgtaa atattgtgaa
    69901 ccgggagcct ggcagcctga aaacgtgcca gcagtttgtt gattcggaat gccgacagtg
    69961 caggcgaacc acgcagaatt tccatcataa gtctctcgtc ttctaagctt tcggtgtacc
    70021 caagggggga aacgggcgtc attataaaga atctgatgcg ctgacgaaac cgtttgcgtg
    70081 gaaataaaat caccatcgtg aattagcaac gcgtgccgcc aatggctgta ataagttgcc
    70141 atctggcgca ggtttacgca aaatgccgct catttatgag taaacctttc actattatta
    70201 cattttttca agctgggacg cgcacgacac agagaattaa ctaattgaaa aaattaaaga
    70261 ttaattatct gttcatcggc attctggcac tgctgctcgc ggtcgctctc tggccatcca
    70321 ttccctggtt tggtaaagcc gacaaccgta tcgccgccat tcaagcgcgg ggagagttgc
    70381 gtgtgagcac cattcatact cccctgacgt ataacgaaat caacgggaaa ccttttggcc
    70441 tggattacga actggcgaaa cagtttgccg attacctcgg cgtaaaactg aaagtgaccg
    70501 tgcggcagaa tatcagccag ctgtttgacg acctcgataa tggtaacgcc gacctgctgg
    70561 cggcaggact tgtctataac agtgagcggg taaaaaatta tcagcctggc cctacctatt
    70621 attccgtgtc acaacaactg gtttataaag tgggtcagta tcgcccacgt acgctgggca
    70681 acctgacggc ggagcaactc accgttgcac cgggtcatgt ggtggttaac gatctccaga
    70741 ccctgaaaga aacaaaattc ccggaattaa gctggaaggt agacgacaaa aaaggctctg
    70801 cggaattaat ggaagatgtc atcgaaggaa aactcgatta caccattgct gattctgtcg
    70861 ccatcagcct gtttcagcgc gttcacccgg agctcgccgt agcgctcgat atcaccgatg
    70921 aacaaccggt gacctggttt agcccgttag atggcgataa taccctttcc gccgccctgc
    70981 tcgacttctt caacgaaatg aatgaagacg gtacgctggc acgcattgaa gagaaatacc
    71041 tggggcatgg cgatgatttt gattacgtcg atacgcgcac atttttacgc gccgtcgatg
    71101 cggtactgcc gcagttaaag cccctgtttg agaaatacgc cgaagaaatt gactggcgtt
    71161 tgctggccgc tattgcttat caggaatcgc actgggatgc acaggccact tcaccgacgg
    71221 gtgtgcgcgg catgatgatg ttaaccaaaa ataccgcgca aagcctcggc attacggatc
    71281 gtaccgatgc cgaacagagc atcagcggtg gcgtgcgtta tttgcaggat atgatgagta
    71341 aagtgccgga aagtgtgccg gagaacgagc ggatctggtt tgccctcgct gcgtacaata
    71401 tgggctatgc gcatatgcag gatgcccgcg ccctgacggc aaaaaccaaa gggaatcctg
    71461 acagttgggc tgacgtaaaa cagcgtctgc ctttacttag ccagaaaccc tattacagca
    71521 agctgactta cggctacgct cgtgggcatg aagcctacgc ttatgtcgaa aatattcgta
    71581 agtatcagat tagcctggtg ggttatctgc aagagaaaga gaagcaggct acagaagcgg
    71641 cgatgcaact ggcgcaggat tatccggcgg tatcgcctac ggagttgggc aaagagaaat
    71701 ttccttttct ctcgtttctt tcccagtcgt catcaaacta tttgacccat tctccctctc
    71761 tgctgttttc caggaaaggg agtgaagaga aacaaaatta atccgtcgag gattgcgctt
    71821 ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa gtcactgagc aacgccgcgc
    71881 actcatccgc cagtattcct tccgtaattt ccactcggtg attcataccc ggatgatgca
    71941 gcacatccat taaagatccc gcagcgccag ttttcgcgtc acgcgcacca aagaccacgc
    72001 gaccaatgcg actgtggatc atcgctccgg cacacattac acatggttca agcgtgacat
    72061 acaacgtggc gtcgatcaga cgataatttt gcatcaccag accgccctgc ctcagggcca
    72121 tgatttctgc atgtgcggtg ggatcatgtc gaccaatcgg acggttccag ccttcgccaa
    72181 taacacgatt gttatgcact aataccgcgc cgaccggtac ttcccgctca tcccaggcac
    72241 gcttcgccag cgtcattgca tgacgcatcc agtattcgtg gctaaattcg acttcagaca
    72301 aaaagaaaac tccggttata aaagcgcggc gcattataca cggacgctat gctttactct
    72361 attccagttg ctggagttca ccgcgcgggg ttactcgcca gcgatgctgg caaaagtaaa
    72421 gcagcgggtt gtcctgatta ctgtcgctat agccactgta cagccgcagc ggcgtgccga
    72481 ttttgcgctc cagttgcgcg accttttcat gcccgaggca acgcatcgtc aatacccaac
    72541 caccatagcc acgctgaatt tggctggcga taagattcac ccgcggcagc cagggtgtat
    72601 cgaaatagac cgcttcaacc agcggctgtg gagaaccggt aatcaaccag atatcagcat
    72661 cggaacttaa caggtaggtg gttaatcgct cctgaaccag cggaaaggcg gtaacattgt
    72721 cacgaaacca gcgtacgaaa tcggcctgca acgcctgtaa acgtgcttcg ctgtgaccaa
    72781 aggtgcatcc ccacagaagc agactcatcg gccagcgtgc cgcacgacct tttatcaata
    72841 acgcaatggc tataatcggc aacaacggca ggacaagtaa cgcattcagc ggttggcgac
    72901 gcagtaaata gcgcagaaaa ctgccgaaca tatcctgctg atgcaatgtt ccatctaagt
    72961 caaaaaacac cacacgacgc tcgtgagttg ccaaaccata ctcctctgga ctgaaacatc
    73021 ctgattaatt ttcgctacta catagcctaa cagatagatc atcacttttc cggcaacaat
    73081 cgaccgcatc atggctaact ggaattttta attcatggca attagcggca atggaatata
    73141 aaattcactc gcgtgtgtct catattgcct gttgtcgcca ctatttcatc gcgaggaaag
    73201 atatgaacgg cttacttcgt atccgtcagc gttaccaggg gcttgcccag agtgataaaa
    73261 aactggcgga ttatctgctg ctacaacctg atacggcgcg ccatttaagc tctcagcaac
    73321 tggccaacga agccggagtc agtcagtcca gcgtcgtgaa gttcgcgcaa aaactcggct
    73381 ataaaggttt tccggcgctt aagttggcgt tgagtgaagc gctggcaagc cagccggaat
    73441 cgccctcagt gcccattcat aaccaaatcc gcggtgatga tccgttacgg ctggtcggcg
    73501 aaaaactgat taaagaaaat actgccgcta tgtacgcaac gctaaacgtt aatagtgaag
    73561 agaaactgca tgaatgcgta acaatgttgc gctctgcgcg gcggataatt ctgaccggta
    73621 ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa gctgatgaag attggcttca
    73681 atgctgccgc agtgcgcgat atgcatgcgc tactcgctac cgtacaggcg tcgtcccctg
    73741 acgatctgtt attagccatt tcttacaccg gtgtacgacg cgagttaaac ctggcggcag
    73801 atgagatgct gcgagtgggc ggaaaagtgc tggcgattac cggctttact cccaatgccc
    73861 tgcaacagcg tgcttctcat tgcctgtata ccattgccga agaacaagcg acaaacagtg
    73921 cttcaatctc tgcttgtcac gcgcagggaa tgttaacgga tttgctgttc attgcgctga
    73981 ttcagcagga tctggaactg gcaccagaac gtattcgtca tagtgaagcg ctggtgaaaa
    74041 aactggtctg agtaaagaat gcgcgtataa tgcccgcccg gtttgtgttg ttttgagagt
    74101 ttccttatgg cgctgttaat cactaaaaaa tgcatcaatt gtgatatgtg tgaacccgaa
    74161 tgcccgaatg aggcgatttc aatgggagat catatctatg agattaacag cgataagtgt
    74221 accgaatgcg tagggcacta cgagacacca acctgccaga aggtgtgccc gatccccaat
    74281 actattgtga aagatccgca acatgtcgag acagaagaac agttgtggga taaatttgtg
    74341 ctgatgcacc acgcggataa aatttaatat tctactctgg aagtagagta ttaattattt
    74401 tactgggaag ccttaacgcc attatattta tttaattgat gacattagca taataattca
    74461 ctaagttaat ttatatagta tctgcccaga cacttattta tagttattaa aggcgcgtcc
    74521 gattggttca ccggacgcac cttaagtacg tttccttgtg ttataagaac agaaggatca
    74581 gctgtaaaac agcaatgatg attttgatga cccgtttaat caggtatcgg caatcagtca
    74641 ttcgtttttc cttaaacaag gaatgcagcc gttcagatta gcccttacat ctccccaaaa
    74701 ctgaacgtgc gagttattgg aatcgagtgc tgcactccac accagagctt tgacgacacc
    74761 actcgtttca atgggggaat tcagtggcat ggtgtaaagc acagcaaaat tgccaataac
    74821 gaaaccgatt ttaatgtact tatcaaaaag cggtcacgtt aatcttttca tcaatgtcga
    74881 tttcttcgta aaggctcgcg ttttttatgt cagagattgc aagttgcttg tacaggtcaa
    74941 cgaaattgat ccataatcat tttcgttgga atcgtgtgct gcacaaaatt aaagttaaaa
    75001 agtaaaaccc ccgttcctta ccagttcggg ggttttactt tataaagaga acggtattat
    75061 ttttaacttt caataattac cgtggcacaa gcatagtggc gctcatcggc cagcgtcaca
    75121 tgcatatttg caacgcccag cttttccgcc agttttaatg cctcgcccca cagtcgcagc
    75181 cgtggcttac caagctcatc gttaaatact tcaaactgat taaacgccag accattgcgg
    75241 atcccggtgc caaacgcttt tgctgcggct tcttttacgg caaagcgttt cgccagaaaa
    75301 cgcaccggct ggtggtgcgt tttccaaatt tcccattcgt tatcgcttaa tacgcggcgt
    75361 gccaggcgct caccggatcg ggcgatcact gcttctatgc gagcgatctc tacgatatcc
    75421 gtgcctaaac ctaatattgc cattagccac gcgcttccag catcaggcgc ttcatttctg
    75481 ccaccgcatc tttcagtccg gtcatcactg cacgaccaat aatggcatga ccgatattca
    75541 gttcgtgcat ctcagggatg gcggcaatgg cttttacgtt gtgataggtc agaccgtgtc
    75601 cggcgttaac tttcaaaccg agactagcgg caaaggtcgc ggctttggcg atacgcgcca
    75661 gctcttgcgc ctgttcggcg tcagttttgg catcagcata gcaaccggtg tggatctcga
    75721 taaacggtgc gccaacctct gccgcagctt tgatctgctc ttcatcggcg tcgataaaca
    75781 gagaaacctg aatcccggca tccgccagac gtttgcaggc atcgcgcatt ttgtcacgct
    75841 gccctgcaac gtccaggccg ccttcggttg ttacttcctg acgcttttct ggtaccaggc
    75901 agcaaaaatg cggcttcgtc tcaaccgcga tcgccagcat ctcttcggtc accgccatct
    75961 ccagattcat gcgggtatcc agcgtctgac gcaggatgcg cacatcgcgg tcagtaatgt
    76021 ggcggcgatc ttcacgtaaa tgcacggtaa tgccgtccgc ccccgcctgc tcggcaataa
    76081 acgcggcctg caccggatcc gggtaagcgg taccgcgcgc gttgcgcagc gtagcgatat
    76141 ggtcaatgtt gacgcctaac agtaattcag ccatgacaat cctcatcatt cataatgtgt
    76201 tttcaccgtt cgcttaggca taaattgccg gaacagttcc ctgctcttta aaggtttacc
    76261 gccaagatac ggcttaagcg ccatgcgggt aaagcgtttc gcggcgcgca gtgtgtctgc
    76321 gtcaggaaat tcccgtgcgt ttaacgcttt taactgcctt ccggtgaacg ttttattgtc
    76381 gataacgacg cttgcgataa accctttttc ttcgcgataa cgatacgtca tggtgtcatc
    76441 taccggctcg ccgctacccg cacaatgggt aaaattgacg ccataaccca gatgcccgag
    76501 cagtgccagt tcaaagcggc gcagcgcggg ttctggcgta ccagtgaccc ctgcaagaga
    76561 ctgaatgcag tgcaagtaat cgaaaaagag ttcagagaag cgcgtctcgt attccagtac
    76621 gcgggagaga agttcgttga tgtacagacc gctgtaaagc gtgataccgc ttaatggcag
    76681 cgccagcgag acagcttcag cactgcgcag cgttttgact tcgccacgcc cgccaaaacg
    76741 tagcaagaga ggggtgaaag gctgtaatgc acctttcagg gtagagcgtt tagagcgtgc
    76801 gcctttggca accagacgca cgcgacccga ttcctccgtg aagacgtcca gcatcaggct
    76861 ggtttcgctc cacgggcgac tatgcaggac aaatgcgcgc tgccagcctt ccatcggagt
    76921 tactcttaaa gatcgtcaac gtaaccgaga ctgcgcagtg cgcgttcgtc gtcggcccaa
    76981 ccggatttca cttttaccca cagctcaagg tgaacaggcg cttcaaacat ttcctgcatg
    77041 tctttacgcg cttcaatccc gatggttttg atcttggccc ctttgttgcc aatgaccatc
    77101 ttcttctgcc cttcacgctc aacgagaatc aaaccgttga tgtcataacc accgcgttcg
    77161 ttagagacga aacgttcgat ctccacggtc acggagtacg gcagttcagc gccgaggaaa
    77221 cgcatcagtt tttcgcggat gatttcagac gccataaaac gctgtgagcg atcggtgatg
    77281 taatcttccg ggaagtgatg agtcgcttca ggtagatgct tacgcacgat tgccgcaata
    77341 gtggcaacat tcagcccggt ttcggcagag attggcacga tatcgaggaa gttcatctgg
    77401 cttgccagga actgcaggtg cggcagcaga tcggctttct cctgcacgtt gtccactttg
    77461 ttcaccgcga ggattaccgg cgctttgcct tcgcgcagtt tgttgagcac catttcgtcg
    77521 tccggcgtcc agcgggtgcc ttcaacgaca aaaatcacca gctcaacatc gccaatagag
    77581 ctgctcgccg ctttgttcat caggcggtta atagcgcgtt tttcttccat atgcaggccc
    77641 ggtgtatcga cgtagatcgc ctgatacgcg ccttcagtat ggatccccac aatgcggtga
    77701 cgagttgtct gcgccttgcg ggaggtgatg gagattttct gccccagcag tttgttcaac
    77761 aatgtggatt tgccaacatt cggacgtccg acgatggcaa taaatccgca gtaactttta
    77821 tcgatgctca ttccagctcc agttttttca acgcctgttc ggcggcagcc tgctcagcct
    77881 tacgacggct tgaacctgtg ccaaccaccg gttcactcag gccgctgacc tggcagtgga
    77941 ttgtaaattc ctgatcgtgc gcttcgccac ggacctgaac taccagataa gtcggcagcg
    78001 gcagatgacg accctgcaaa tattcttgca agcgcgtttt cggatctttt tgtttatcgc
    78061 ctgggctaat ttcgtccaga cgagtttgat accagttgag gattaatttc tcgacggttt
    78121 gaatatcact gtcgaggaat acgccaccaa ttaatgcttc gacggtgtcg gcgagaattg
    78181 actcacgacg aaatccaccg cttttaagtt cacctggtcc taaacgtaag cactcgccta
    78241 actcaaattc gcgcgccagt tccgccagcg tattgccacg gaccagcgtg gcgcgcatcc
    78301 ggctcatatc gccttcatcc acacgaggga aacggtgata aagcgcattg gcgataacgt
    78361 agctcagaat agagtcgcct aaaaattcta aacgctcgtt atgtttgctg ctggcactac
    78421 gatgagttaa tgcctgctgc aacagttcct gatgattaaa agtgtagccc agcttccgtt
    78481 gaagccgatt aattacgatg gggttcatgc gataccaata aataaatgcg tcaacaattc
    78541 agcacacgaa acagacctga tatacatggc tctgctaact gcttcgctgc agtttcagtt
    78601 tagcggtata tggggaccaa cgctgtttcg tgtgccgtgg caacctggag gtgccaacct
    78661 taaacttcgg gggaatattc tatacacaac gacgggggat gtcgttagcc acgggagatt
    78721 tatctcataa ataattcacg ttgtcgccat aacggcgaca acgtgaacga agatgagcta
    78781 ttaatggatg ccgccaatgc gacttaagcg cacacctgtc ggccattcgc cttcttgctt
    78841 atcgaagctc atccagatag ccgttgcccg accgaccaga ttcgcttcag gcacaaagcc
    78901 ccagtaacgg ctgtccgcgc tgttgtcgcg gttgtcgccc atcatgaagt attgtcccgg
    78961 aggaacaatc caggttgcca gttgttgccc tggctgctgg taatacatcc ccacctgatc
    79021 ctgcgcaatc ggcactgtca gaatgcggtg cgtcacatca cccagtgtct ctttacgctc
    79081 ggaaagacga attccatttt ctttggtttc gtttttcggc acttcaaaga atccgctggt
    79141 cgcttcccca ccattacggc gtgagaaggt ctgaacgaaa tcgctcggtt ccacgtttga
    79201 gtaggtgacc ggcagcgcgt tttcacacgc ctggccggaa ctgcatcccg gttgaatcgt
    79261 cagctctttt gagaccggat cgtaagtgac tttatcgccc ggtaaaccca ccgcgcgctt
    79321 gatataatca agctttggat cttccggata tttaaagacc acgatatcgc cgcgtttcgg
    79381 atgaccggtt tcgatcagcg ttttctggta gataggatct ttaatgccat aagcaaactt
    79441 ctctaccaga ataaaatcac cgattaacag agtcggcatc atcgaacctg acggaatctg
    79501 gaacggttca taaataaacg aacgcacaat caatacgata gccagtaccg gaaaaacaga
    79561 agcaccggtt tccagccagc caggcttcgg cgcaaccttt ttcaacgttg ctttatccag
    79621 tgagtcaccg gcagccgcct gcgccgctgc ctgacgttcc cgccgtttag gtgcgaaaaa
    79681 gaatttatcc acgcaccata aaatgcccgt caccagtgtg gcaatcacca gaatcagggc
    79741 aaacatattc gccatgccaa ctcctaaggg ttatttgttg tctttgccga cgtgcagaat
    79801 ggcgaggaac gcttcctgcg gcagctcgac gttaccgatc tgcttcatgc gtttcttacc
    79861 ttctttctgc ttctgcagca gctttttctt acggctgata tcgccgccat aacatttagc
    79921 cagtacgttt ttacgcagct gtttcacggt ggatcgcgca ataatgtgcg taccaatcgc
    79981 tgcctgaatg gcgatatcaa actgctggcg tgggatcaga tctttcatct tctccaccaa
    80041 ctcgcgaccg cggttttgcg aattatcacg gtgggtgatc aacgccagcg catcaacacg
    80101 ttcaccgttg attaatacgt ctacacgtac catgtcggac gcctggaagc gtttgaagtt
    80161 ataatccaga gacgcataac cacgcgaggt agatttcagg cgatcgaaga agtcgagcac
    80221 cacttccgcc atcgggatct cgtacgtcag cgccacctga ttaccgtggt aaaccatatt
    80281 ggtctgcacg ccgcgttttt ctacgcacag cgtaataacg ttgccgagat atgcctgcgg
    80341 cagcagcatg tgacactctg caatcggctc gcgcagttcg taaatgttat ttaccgcagg
    80401 cagcttggat gggctgtcga cgtagataac ttcccttgac gtggtttcaa cttcatacac
    80461 tacggtcggc gcagtggtga tcagatccag atcgtattca cgttccagac gctcctggat
    80521 gatctccatg tgcagcaggc caaggaagcc gcagcggaaa ccaaagccca gcgcgctgga
    80581 gctttccggc tcatagaaca gtgaggcatc gttcaggctg agtttaccca gcgcgtcacg
    80641 gaaggcttca tagtcgtcgg aacttaccgg gaacagaccg gcgtatacct gcggtttgac
    80701 tttcttaaag ccaggcagcg ccttttctgc cggattacgc gccagcgtta aggtatcgcc
    80761 gactggagcg ccgtggatat ctttaatcgc acatacgagc cagcctactt cgccacattt
    80821 cagttcagtg cggtcaacct gtttcggcgt gaagatgccc agacggtcgg cgttataggt
    80881 ctgcccggta ctcatgactt tcactttgtc gcccttacgc agggtgccgt ttttaatacg
    80941 gataagtgaa acaacgccca ggtagttgtc gaaccatgag tcgataatta gtgcctgcaa
    81001 cgggccttcc ggatcgcctt ccggcggcgg aatgtcgcgc accagacgtt cgagaacgtc
    81061 ctgcacgcca acgccggttt tcgctgaaca gcgcaccgcg tcagtggcgt cgatgccgac
    81121 gatatcttca atttcttccg ccacgcgttc aggatcggct gccggcaggt caatcttgtt
    81181 cagtaccggc accacttcga gatccatttc catggcggtg tagcagtttg ccagggtttg
    81241 cgcttccacg ccctgcccgg cgtcgaccac cagcaatgca ccttcacagg cagccagcga
    81301 acgggaaact tcataggaga agtctacgtg gcccggggtg tcgataaagt taagctgata
    81361 ggtttcgccg tcagacgctt tgtagtccag cgtcacgctt tgcgctttga tggtaatgcc
    81421 acgctcacgc tcaagatcca tggaatcgag aacctgcgcc tccatttcac ggtcagacag
    81481 gccaccgcag atctggataa tacggtcaga cagcgtcgat ttaccgtggt caatgtgagc
    81541 tatgatcgaa aagttacgta tattcttcat aaagtatgat tattgtgcct taatgcccgg
    81601 ttaaccaggc ttttagaagt cgctgttctg agcttaacgt ctgtattaat agaaacgccg
    81661 cattctacac tacaacattg aggcgaggaa atgttcatac cgtatggatt gtggtatctg
    81721 gaaacgtcct cgcatttgtt atgcaaaatg caacaaagcc agtgaaatca ctggctcgcg
    81781 tcttccgaag atgtttcaaa tcgcacaagg ccaggcggca atgccacgct taagatgatc
    81841 ggttgccatt ctgcccgggc agcaaactta cgcgagtagc cacgcgcaat caggaacccg
    81901 ccaataccgc cgaggaccgc accacataat gctgcgacgt cagaagcaaa gagtagctga
    81961 aatagcgaag cgatgaggaa taatcccacc agcggcgaca tataaaccag taatgcggag
    82021 ctaagcaggc tgccttcggc gatccccaat tccacttttt gccccggcac taacggctca
    82081 tcacagggta cgacaatggt atgcgtggtt tgcgggccaa gtttattcaa cacgcggcta
    82141 ccgcaaccgg cgcgtgaagc gcagctgctg catgaggctt taacatcaca actgaccagc
    82201 gcctgcccgt tttgccagga gacgacggta gcccactctt tgatcattgc gctgccccga
    82261 acttaatatt ctcggcaatg cgtttcgccg tttgcggcgg cagttcaccg acaatggtga
    82321 tttcggcgtt atcacgtacg cttgtactga cggttctgcg tccggtgcgc aacatctgat
    82381 cggtgctcga tggcgtagcg cggttaacgt ttaccgagaa gctgaataat ccgtcggaat
    82441 agagacgtga ttcgataggc atgttgtcca tcgtcggtag cggacgtcga ctactggaaa
    82501 cttcgctaaa accctgtggc aaccaggttg gcgtccagct gaatttagct ttttcaccta
    82561 caggaacaga aagcaacggc ggcaaatttg ccttcgccag cgtctgcata ctgctgctga
    82621 tatcctgatt gacgttaaaa gcaatcacgc gaaattgttc cagcgtttca ccatcgcgat
    82681 caagaagatc aacccgcatc ggtaatttcg attcggtgtc catccacacg atgtagctgt
    82741 agcgtgtacc atctcgggca accacgcgaa tgacttcgca aagacgatca gcaatacgcg
    82801 tgcgcccgac ggagataaag tcgtagtaag gagaaaggcg tttgaaatcg gtatagatga
    82861 gcgatggcag agaatcaaca atgtaatcgc cattaagcgt gaacggttca agtcccggtt
    82921 caaaatagct gatttcattg ccgcgctgta ccacttcccg gcgcgggcca tccatttgca
    82981 acaattgtgc aagaggacgg ttatcgaggc gtgcatgtcg ataacgcaga gactcaacac
    83041 cctgtttatt gatgctgatg aatgacagct cgtaattcag tgactgactg gccaggttca
    83101 tctgctgtaa taacgccccg gacgcgggag tggccgaggc gttagcagag aataacaggc
    83161 tacctgtcac taatgacatg gcaaaccaaa gttgcttcat tactgcgatt gcgttcctaa
    83221 agtttgaatt cctggcacct gtacagcggc ttgctgggtt tgcgcctgct caaactgaag
    83281 ctgttcagag tggagtcggc gttgcagttc gtaatcctgc aacattgcat taatgcgacg
    83341 acgctgctgc tgttgaccat tatttgcggt cgcttcagaa ggtactccca ggctaaccgg
    83401 gctggcttta cccatcatcg gcagtgtatt aaataccggc gtttcgggct gctgggacgt
    83461 ttcagattgt ccattatagt gctggacgcc aacgataact gcaagcgata cgcatgcggc
    83521 tacgcccatt tgggtaagct gtgccgccca cggacgtact ttctgccaga atggcatttt
    83581 ctgccattca tgcggcgcag gctgggcttc cgggatcaat gtcgccggtt gacgtactgg
    83641 ctcttcttca atggcggcca tcacgcgtga agagatatcg aaatggagca cctcgggagt
    83701 atcaccccgc attgagtcac ggattaagtg atagctttcc caggttttct gcatttctgg
    83761 gttatgagcc agttcgttaa gcagctcact atccagcgtt tcgccatcca ttaaagcgga
    83821 aagttgttct ttctgcatgc ctaataccct tatccagtat cccgctatcg tcaacgcctg
    83881 ataagcggtt gaactttgtt atcaatagct tccctcgctc ggaagatacg tgaacgcacc
    83941 gtacctaccg gacaatccat gatagcggct atctcttcat agctcaggcc atccagctcc
    84001 cgcaaggtta ttgccatgcg taaatcttcc gggagggact caatagttcg gaaaactatc
    84061 tgtctcagtt cttctgacaa cattaagttc tcagggttcg aaatttcttt caacgcgccg
    84121 ccactttcga agttttcagc ttcaatggca tccacatcac tggaaggtgg acgacgcccc
    84181 tgagcaacca ggtaattttt cgctgtattt acagcaatcc gatacagcca tgtataaaaa
    84241 gcgctatctc cccggaacga atccagcgca cgataggctt taataaaagc ttcttgtacc
    84301 acatcgggaa catcacccga cggcacatag cgggaaacca gactcgccac tttatgctga
    84361 tagcgcacta ccagtaagtt aaaggctttc tgatctccct tctggacccg ttcaaccagg
    84421 acctggtccg ttaactgctc gctcatccga ggtaaagtct ccccaaacca aatttccacg
    84481 cgctatcgaa acgccactcc attagctgca atttgagcaa gcaaagggtt agagtgtctc
    84541 gtttttgtaa agttccgtaa cgcatctgtt tttgtttgtc atgctgtaga cggatcatta
    84601 tctatcatta taagtctaca gaatctgaac atcgcattat ctgtgtagaa atgcccattt
    84661 aactgcctga agagtaaccc aacggccttt ttatttcacc acctaatcct ccaccagcca
    84721 gtaacttctc tttttctcgc cgccctgcgt cagcgtgttt agtcactgta acaaatatta
    84781 aaatagcagg tgtttattcg cacaacatga tgctatgctg accaaaccgt gtttagtaaa
    84841 ttaaacaaag aaaatgaata ctctccttga acattcatgt gacgtgttga ttatcggtag
    84901 cggcgcagcc gggctttcac tggcgctacg cctggctgac cagcatcagg tcatcgttct
    84961 aagtaaaggc ccggtaacgg aaggttcaac attttatgcc cagggcggta ttgccgccgt
    85021 gtttgatgaa actgacagca ttgactcgca tgtggaagac acattgattg ccggggctgg
    85081 tatttgcgat cgccatgcag ttgaatttgt cgccagcaat gcacgatcct gtgtgcaatg
    85141 gctaatcgac cagggggtgt tatttgatac ccacattcag ccgaatggcg aagaaagcta
    85201 ccatctgacc cgtgaaggtg gacatagtca ccgtcgtatt cttcatgccg ccgacgcaac
    85261 tggtagagaa gtagaaacca cgctggtgag caaggcgctg aaccatccga atattcgcgt
    85321 gctggagcgc agcaacgcgg ttgatctgat tatttctgac aaaattggcc tgccgggcac
    85381 gcgacgggtt gttggcgcgt gggtatggaa ccgaaataaa gaaaaggtgg aaacctgcca
    85441 cgcaaaagcg gtggtgctgg caaccggcgg tgcgtcgaag gtttatcagt acaccaccaa
    85501 tccggatatt tcttctggcg atggcattgc tatggcgtgg cgcgcaggct gccgggttgc
    85561 caatctcgaa tttaatcagt tccaccctac cgcgctgtat cacccacagg cacgcaattt
    85621 cctgttaacg gaagcactgc gcggcgaagg cgcttatctc aagcgcccgg atggcacgcg
    85681 ttttatgccc gattttgatg tgcgcggcga actggccccg cgcgatattg tcgcccgcgc
    85741 cattgaccat gaaatgaaac gcctcggcgc agattgtatg ttccttgata tcagccataa
    85801 gcccgccgat tttattcgcc agcattttcc gatgatttat gaaaagctgc tcgggctggg
    85861 gattgatctc acacaagaac cggtaccgat tgtgcctgct gcacattata cctgcggtgg
    85921 tgtaatggtt gatgatcatg ggcgtacgga cgtcgagggc ttgtatgcca ttggcgaggt
    85981 gagttatacc ggcttacacg gcgctaaccg catggcctcg aattcattgc tggagtgtct
    86041 ggtctatggc tggtcggcgg cggaagatat caccagacgt atgccttatg cccacgacat
    86101 cagtacgtta ccgccgtggg atgaaagccg cgttgagaac cctgacgaac gggtaataat
    86161 tcagcataac tggcacgagc tacgtctgtt tatgtgggat tacgttggca ttgtgcgcac
    86221 aacgaagcgc ctggaacgcg ccctgcggcg gataaccatg ctccaacaag aaatagacga
    86281 atattacgcc catttccgcg tctcaaataa tttgctggag ctgcgtaatc tggtacaggt
    86341 tgccgagttg attgttcgct gtgcaatgat gcgtaaagag agtcgggggt tgcatttcac
    86401 gctggattat ccggaactgc tcacccattc cggtccgtcg atcctttctc ccggcaatca
    86461 ttacataaac agataaaaag cctgggttag cgccgtatac gcttcggaat agttctggtc
    86521 tggcccacga atgactaagc gatcgctaaa gcattctccc gcctgcgggg agaatgccag
    86581 cagcacccga tgcggcagtc gcgcttcgtt ttccgccaca tccgtccgca aacgtaaatg
    86641 ccagcccata cttaatgcca gctccgtaaa actattacca atctgctctg gcagcaccac
    86701 gcagaaaaat ccctcttcgg taatgcactc cgccgcgcaa gtaagtaaag tctggtgatc
    86761 aagtgaggtg gtgtagcgcg cctgttcccg ctgcggcgtc gcgcactcca ctccttgctc
    86821 ataatacggc gggttgctga tgattaaatc aaagcgcgcg gtctgctgtg ttacccactg
    86881 ctgaatatcc gccgtatgga cgttaattct ctctaaccac ggggactggg cgacattttc
    86941 ctgcgcctga gtggcagctt cactttccag ctcaacggca tcaattatca cgctgtcact
    87001 ggttcgctgc gccagcatca gtgccagcaa gccactaccc gcaccgatat caaggcaacg
    87061 ttttacccca gccaccggtg cccatgcgcc cagcaatatg ccatccgttc ccgctttcat
    87121 cgcacagcga tcgtgagcaa caaaaaactg tttaaaagta aatccattac gacgaagcac
    87181 ggatgtagac tgtgacatga aaataaaacc ttgcaggaaa aacggcgata gcaccgggtg
    87241 agaacaatac ccgagaagcg atatccatac aaacagatga agattgcagc cgtaacgtct
    87301 ataatcagcg ccccacacag aggtagaaca tgactgtaac gactttttcc gaacttgaac
    87361 tcgacgaaag cctgctggaa gccctccagg ataaaggttt cactcgcccg accgccattc
    87421 aggctgccgc cattccgcct gcgctcgatg gccgtgatgt actcggttct gcgccgacag
    87481 gcaccggtaa aacggcggcg tatctgctgc cagcgttgca gcacctgctc gatttcccgc
    87541 gtaagaaatc cggtccgccg cgtattttga tcctcacccc aactcgcgag ctggcgatgc
    87601 aggtggccga tcatgcccgc gaactggcga aacatacgca tctggatatc gccaccatca
    87661 ccggcggcgt agcctatatg aaccacgcgg aagtgttcag cgaaaatcag gacatcgtgg
    87721 tcgccacgac cggacgtctg ctgcaataca taaaagaaga gaacttcgat tgccgcgcgg
    87781 ttgaaacgct gatcctcgac gaagcagacc gtatgctgga tatgggcttc gctcaggata
    87841 tcgaacatat tgctggcgaa acgcgctggc gtaaacagac cctgctcttt tcggcaacgc
    87901 tggaaggcga tgcgattcag gactttgccg agcgtctgct ggaagatccg gtggaggttt
    87961 ctgccaatcc gtccacccgt gagcgcaaaa aaattcatca gtggtattac cgcgccgatg
    88021 acctggaaca taaaaccgca ttgctggtgc atctgttaaa acagccggaa gcgacccgct
    88081 caattgtgtt tgtgcgtaag cgtgagcgtg tgcatgagct ggcaaactgg ctgcgcgaag
    88141 cgggcatcaa caactgctat ctcgaaggtg agatggtaca gggcaagcgt aacgaagcga
    88201 tcaaacgttt gaccgaaggt cgcgtaaacg tactggttgc aaccgatgtt gccgcgcgcg
    88261 gtatcgacat tcctgacgtc agccacgtct ttaacttcga tatgccgcgc agtggcgata
    88321 cttatttgca ccgtatcgga cgtaccgcgc gcgccggtcg taaaggcacc gcaatttcgc
    88381 tggtagaagc ccatgaccat ctgctgctgg gcaaagttgg tcgctatatt gaagagccaa
    88441 ttaaagctcg cgtaattgat gagttacgcc cgaaaacgcg tgcaccaagc gagaagcaga
    88501 ccggcaagcc atcgaagaaa gtactggcaa aacgcgccga gaagaaaaaa gcaaaagaga
    88561 aagagaagcc gcgggtgaaa aaacgccatc gcgataccaa aaatattggt aagcgccgta
    88621 aaccaagcgg aacgggcgtg ccaccgcaaa cgacagaaga gtaatctcaa tgccgggttt
    88681 aagcccggca ttatttttat cccggcaaaa aacaatcttc aacacactga ataaaagtgt
    88741 gcatcgccgg gctaaccgct tttccggcat ggtgagcgca cattgccgta atggtctgcg
    88801 agtgttcgcc aaaaggcaat tcaattaatt ctccgctctc aagctctttt tccaccgcaa
    88861 agcggggcaa gtaactgaca ccgatattcg ccgcgacaca acgtttgata ctttcaatgc
    88921 ttaacagctc aatggtgttt tccaccgtga tccgccgctg gcgcagcgtg ctctcaaata
    88981 tctgccggaa tacacattgc ggttcgttga taataaagct acaggcgtta tgtcttcccg
    89041 gctcagtaaa atcgacatct gcaatttgcg gtgaagctac cagcaccagc gattgctcac
    89101 ccaattctcg tcggttcagg gcatcatcat tccctacgcg ataaaaaacc ccgacatcgg
    89161 cctcatcatt caatagcgca tcacggatca cgtaacagtt cagagactgc aacgataaac
    89221 gcacttttgg ggcgcgttgc cgaaaacgct gcaacacctg cggcattcgg taagagagca
    89281 acgtttcgcc cgaaacaacg cgtagttccc cgtccggatc cgactccttt ttggcagcct
    89341 cacgaagggt atccatcact ctggtgagtt cataaatgtg cggcagcagc tttttacctt
    89401 cgcgagtgag gcacatccgt cggccaattt tctcaaataa ctgtaccgag aactcctgct
    89461 caagctgctg aatatgaaaa gtcaccgtcg attgtgtaca gcacaatttt tgcgaagctc
    89521 gcaaaaagga accctcttcc accacggttt taagcgtaat aaaacggcgc agatccataa
    89581 ccccaaacct atcgaaaata tcgaatttag aatgtaaaaa cattcatttt tttaaatatt
    89641 ctgtgtcggg tactgtctac caaaacagag gagataacaa gtgacaccga cccttttaag
    89701 tgctttttgg acttacaccc tgattaccgc catgacgcca ggaccgaaca atattctcgc
    89761 ccttagctca gccacaacgc acggatttca tcagagtacc cgcgtgctgg cagggatgag
    89821 tctgggattt ttaatcgtca tgttgctgtg tgcgggcatt tcgttttcac tggctgttat
    89881 cgacccggca gctgtgcatc tgttgagttg ggcgggggcg gcatatattg tctggctggc
    89941 gtggaaaatc gctaccagtc caacaaagga agacgggctt cagacaaaac caatcagttt
    90001 ttgggccagc tttgctttgc agtttgtgaa cgtcaaaatc attttgtacg gtgttacggc
    90061 actgtcgaca tttgttctgc cgcaaacaca ggcgttaagc tggatagttg gcgtcagcgt
    90121 tttgctggca atgattggga cgtttggtaa tgtgtgctgg gcgctggcgg ggcatctgtt
    90181 tcagcgattg tttcgccagt atggtcgcca gttaaatatc gtgctggcgc tgctgctgat
    90241 ctattgcgcg gtacgcattt tctattaatg aaaaaaagcg gaagaggtcg ccctcttccg
    90301 cttagtaaca tgctacttaa gccttacagg ctttcagtaa aggtacgagc gataacgtcg
    90361 cgctgctgtt ccggagtcag agagttaaag cgaactgcat aaccggatac acggatggtc
    90421 agctgcggat atttttccgg atgcttaact gcatcttcca gagtttcacg acgcagaacg
    90481 ttaacgttca ggtgctgacc accttcaacg cgaacttctg gtttcacttc tactggaact
    90541 tcacggtact caatgtcacc cagtttgctt actgcaacca cttcgtcttc tgcataacct
    90601 gctttcgcaa cgatgcaacg cgcttcgcct ttttcgctgt ccagcagcca gaaagagttc
    90661 agcagatcgt cgttagcggc tttagtaatc tggatacctg taatcatgtg atgcctcccc
    90721 ggcaaaatta tttgatttgt tcagcctgtc gcggccaatt ggtaaaacca ttgttgcttg
    90781 agtgtatata tactcctcaa acacccttga atctttgatt taaatcaata aaaaccacac
    90841 atcaagtgtg gtcgcaaata gattttattg ttttacatca acttatgcgg gtgtgaaatt
    90901 ttaccaattt acattttttt gcactcgttt aagtctaaaa aatgagcatg attttgttct
    90961 gtagaaagaa gcagttaagc taggcggatt gaagattcgc aggagagcga gatggctaac
    91021 gaattaacct ggcatgacgt gctggctgaa gagaagcagc aaccctattt tcttaatacc
    91081 cttcagaccg tcgccagcga gcggcagtcc ggcgtcacta tctatccacc acaaaaagat
    91141 gtctttaacg cgttccgctt taccgagttg ggtgacgtta aagtggtgat tctcggccag
    91201 gatccttatc acggaccggg acaggcgcat gggctggcat tttccgttcg ccccggaatc
    91261 gccactcctc cgtcactgct gaatatgtat aaagagctgg aaaatactat tccgggcttc
    91321 acccgcccta atcatggtta tcttgaaagc tgggcgcgtc agggcgttct gctactcaat
    91381 actgtgttga cggtacgcgc aggtcaggcg cattcccacg ccagcctcgg ctgggaaacc
    91441 ttcaccgata aagtgatcag cctgattaac cagcatcgcg aaggcgtggt gtttttgttg
    91501 tggggatcgc atgcgcaaaa gaaaggggcg attatagata agcaacgcca tcatgtactg
    91561 aaagcaccgc atccgtcgcc gctttcggct catcgtggat tctttggctg caaccatttt
    91621 gtgctggcaa atcagtggct ggaacaacat ggcgagacgc cgattgactg gatgccagta
    91681 ttaccggcag agagtgagta aatttgcggg aaaatgccgg atggcagagt tgccacccgg
    91741 ctgatttatc aggctttatt ctgacgccac cattcgccaa gaagaacgcc ggttgcgaca
    91801 gagatattca gcccggcaac gttgccagta ccatcaatct tcacgcgcag atcgttcgga
    91861 tcgcgtgcgg catccggtaa cccttcatat tcctgaccca gcaccagtac cattttcgct
    91921 ggcagactgg ttttgaacag cggtttaccc tgctcgctgg aagttgtcac tacggtgtaa
    91981 cccgcctgac ggaaatcatc cagcacgtta acaatgttgt cgccagtaat cggctgaacg
    92041 tgctctgcgc cgccttctgc ggtacggata gccgcccccg actccagcag tgccgcatcc
    92101 tgcaccacaa cacctttcac gccaaagtgc gcgcagctgc gcatcatgcc acccaggtta
    92161 tgcgggttag attcgttttc cagtgccaga acacaatcct gcgcgcctgc ctggcttacc
    92221 cactgctgca cggttgtgcc attacgcttt ttgatcagga agcaaacgcc accgtgatgt
    92281 tccgtgcctg acgcttttgt caattccgct tcatctacca catggtacgc tttgcggttt
    92341 gctgccatcc agcgcaaggc ttctttaaaa cgcggcgtta cactctggat aaaccaggcg
    92401 cgaacaatcg cttccggacg gctctggaac agtgcctgac aggcgttttc gccgtagacg
    92461 cgggtttctt ccgcacgctg acgacgcaac acttccggat caataaaact tttaccactg
    92521 atgccaccgt gatcggcctt ttccggcgtc tcatcacccg gcgcgcggga aaccgtgcgc
    92581 cacggcgaat cttcccactt gcggtcacgg ggctgactct gtttgtcatc gcgggcgggg
    92641 cgacggccac cgtcagcacg agattttcct ggacgcccgc cccctttccc ggtacgcggg
    92701 ttgtgggtac gtttatcaga atcatcatca ctgcggacat acatcacttt gaccttgccg
    92761 cttttacctt tcatttcatc gttcatgctt ttctccacca gcgctgcgcg aagcgcgcag
    92821 attacccgaa gtccgcgcgg ttcgccatga tttcgtacca aagcctgcga ctatcatacc
    92881 tattgaataa aacagattgt tgtctggaac aatgtccccg ataatatgta acatattaga
    92941 aacataccgg cgttgttgcc gataagtctc cttactcatc ccgaggttag ttatgaatac
    93001 cgtttgtacc cattgtcagg ccatcaatcg cattcccgac gatcggatcg aagatgcggc
    93061 aaaatgcgga cgctgcggtc acgacttgtt tgacggagag gtgattaatg cgaccggtga
    93121 aacgctcgac aaattgctga aagatgatct accagtggtg atcgacttct gggcaccgtg
    93181 gtgcggcccc tgccgtaatt tcgcaccaat ttttgaagat gtcgcgcaag agcgtagcgg
    93241 taaagtgcgc tttgtgaaag tgaataccga agctgaacgt gaattgagca gtcgctttgg
    93301 aattcgtagt ataccgacga tcatgatttt caaaaacggt caggttgtcg acatgcttaa
    93361 tggcgcagta ccgaaagcgc cgttcgatag ctggctgaac gaatctcttt aatcttaccg
    93421 gggcgcatct tgtgccccgt tttctcctct gcgacaatgg cgttttttcg acgctctctt
    93481 atgaccgaaa acgctgttct ccagttacgc gccgagcgta ttgcgcgcgc aacacgtcct
    93541 tttcttgccc gcggtaatcg cgttcgtcgc tgccaacgct gtcttttgcc agagaaatta
    93601 tgtctctgtt cgacaattac cccagcacaa gcaaaaagtc gcttctgttt gctgatgttc
    93661 gacaccgagc caatgaagcc cagtaatacc gggcgtctga ttgctgatat tttgcctgat
    93721 accgttgcgt ttcaatggtc gcgtaccgaa ccctcgcagg atttgctgga tctggtacaa
    93781 aacccggatt atcagccaat ggtggtcttt cccgcctcgt atgctgatga gcaacgggaa
    93841 gtgatcttca caccacctgc cggtaagcca ccgctgttta tcatgctcga tggtacctgg
    93901 ccggaagctc gcaagatgtt tcgtaaaagt ccgtatctgg ataatcttcc cgtcatttcc
    93961 gtcgatcttt cccgactttc tgcctatcgc ctgcgtgaag cccaggctga aggccaatat
    94021 tgtactgccg aggtagccat cgcactgtta gatatggccg gcgataccgg ggcggcagca
    94081 gggttaggcg agcacttcac ccgctttaaa acgcgctatc tggcaggaaa aacgcaacat
    94141 ctgggtagca tcacagcaga acagttagaa agcgtttaaa atcattcggt cacttctgcg
    94201 ggagaccggt atgagtcagc gaggactgga agcactactg cgaccaaaat cgatagcggt
    94261 aattggcgcg tcgatgaaac ccaatcgcgc aggttacctg atgatgcgta acctgctggc
    94321 gggaggcttt aacggaccgg tactcccggt gacgccagcc tggaaagcgg tgttgggtgt
    94381 gttggcctgg ccggatattg ccagcttgcc ctttacaccc gaccttgcgg ttttatgtac
    94441 caatgccagc cgtaatcttg ctcttctgga agagctcggc gagaaaggct gtaaaacctg
    94501 cattattctt tccgccccgg catcgcaaca cgaagatctc cgcgcctgcg ccctgcgcca
    94561 taacatgcgc ctgcttggac caaacagtct gggtttactg gctccctggc aaggtctgaa
    94621 tgccagcttt tcgcctgtgc cgattaaacg cggcaagctg gcgtttattt cacagtcggc
    94681 tgccgtctcc aacaccatcc tcgactgggc gcaacagcgt gagatgggct tttcctactt
    94741 tattgcgctc ggcgacagcc tagatatcga cgttgatgaa ttgcttgact atctggcacg
    94801 cgacagtaaa accagcgcca tcctgctcta tctcgaacag ttaagcgacg cgcgacgctt
    94861 tgtttcggcg gcccgtagtg cctcgcgtaa taaaccgatt ctggtgatta aaagcggacg
    94921 tagcccggca gcacagcgac tgctcaacac gacggcagga atggatccgg cgtgggatgc
    94981 ggcgattcag cgtgccggtt tgttgcgggt gcaggatacc cacgagctgt tttcggcggt
    95041 ggaaaccctc agccatatgc gcccgctgcg tggcgaccgg ttgatgatta tcagcaacgg
    95101 tgctgcgcct gccgcgctgg cgctggatgc cttatggtca cgcaatggca agctggcaac
    95161 gctaagcgaa gaaacctgcc agaaactgcg cgatgcactg ccaggacatg tggcaatctc
    95221 taacccgctc gatctgcgcg atgacgccag cagcgagcat tatgtcaaaa cgctggacat
    95281 tctgctccac agccaggatt ttgatgcgct gatggttatt cattcgccca gcgccgctgc
    95341 tcccgcaaca gaaatcgcgc aagtattaat tgaagcggta aagcatcatc cccgcagcaa
    95401 gtatgtttct ctgctgacga actggtgcgg cgagcactcc tcgcaagagg cacgacgttt
    95461 attcagcgaa gccgggctgc cgacctaccg taccccggaa ggaaccatca ctgcttttat
    95521 gcatatggtg gagtaccggc gtaatcagaa gcaactacgc gaaacgccgg cgttgcccag
    95581 caatctgact tccaataccg caaaagcgca tcttctgttg caacaggcga ttgccgaagg
    95641 ggctacgtcg ctcgataccc atgaagttca gcccatcctg caatcgtatg ggatgaacac
    95701 gctccctacc tggattgcca gcgatagcac cgaagcggtg catattgccg aacagattgg
    95761 ttatccggtg gcgctgaaat tgcgttcgcc ggatattcca cataaatcgg aagttcaggg
    95821 cgtcatgctg tacctgcgta cagccaatga agtccagcaa gcggcaaacg ctattttcga
    95881 tcgcgtaaaa atgacctggc cgcaggcgcg ggtccacggc ctgttggtgc aaagtatggc
    95941 taaccgtgct ggcgctcagg agttgcgggt tgtggttgag cacgatccgg tttttgggcc
    96001 gttgatcatg ctgggtgaag gcggtgtgga gtggcgtcct gaagatcaag ccgtcgtcgc
    96061 actgccgccg ctgaatatga acctggcccg ctatctggtt attcagggga tcaaaagtaa
    96121 aaagattcgt gcgcgcagtg cgctacgccc attggatgtt gcaggcttga gccagcttct
    96181 ggtgcaggtt tccaacttga ttgtcgattg cccggaaatt cagcgtctgg atattcatcc
    96241 tttgctggct tctggcagtg aatttaccgc gctggatgtc actctggata tcgcgccgtt
    96301 tgaaggcgat aacgagagtc ggctggcagt gcgcccttat ccgcatcagc tggaagaatg
    96361 ggtagaattg aaaaacggtg aacgctgctt gttccgcccg attttgccag aagatgagcc
    96421 acaacttcag caattcattt cgcgggtcac caaagaagat ctttattacc gctactttag
    96481 cgagatcaac gaatttaccc atgaagattt agccaacatg acgcagatcg actacgatcg
    96541 ggaaatggcg tttgtagcgg tacgacgaat tgatcaaacg gaagagatcc tcggcgtcac
    96601 gcgtgcgatc tccgaccctg ataacatcga tgccgaattt gccgtgctgg ttcgctcgga
    96661 tctcaaaggg ttaggcttgg gtcgacgctt aatggaaaag ttgattacct atacgcgaga
    96721 tcacggacta caacgtctga atggtattac gatgccaaac aatcgtggca tggtggcgct
    96781 ggcccgcaag ctcgggttta acgttgatat ccagctcgaa gaggggatcg ttgggcttac
    96841 gctaaatctt gcccaacgcg aggaatcatg agtaaggtac tggaaatgtt gaccacttaa
    96901 tcgggactgg tgttattatt gcacgcttat gtcgtctgca ttgcacagag gacccttcaa
    96961 tgaacagaga agaaatgcac tgtgatgttg tcaaaattta agcgtaataa acatcaacaa
    97021 caccttgccc aactacccaa gatttctcaa tcagttgatg atgtcgattt cttttacgct
    97081 cccgccgact tccgggagac gctgctggaa aaaatagcca gcgcgaagca gcgcatttgc
    97141 attgtcgccc tgtatctcga acaggatgac ggtggcaaag gcattctgaa cgcgttgtat
    97201 gaagctaaaa ggcagcgtcc ggaactggat gtgcgggtgc tgctcgactg gcatcgtgca
    97261 caacgtggac gcattggcgc tgcggcatct aacactaacg ctgactggta ctgtcgcatg
    97321 gcgcaggaaa atccgggcgt agatgttccg gtttatggcg ttccaatcaa tacccgtgaa
    97381 gcccttggtg ttctgcactt taaaggcttt atcatcgacg atagcgtact ttatagcggt
    97441 gccagcctga acgatgttta cctgcatcag cacgataaat atcgctacga ccgttatcat
    97501 cttatccgta accgtaagat gtcagacatt atgtttgaat gggttacaca gaatattatg
    97561 aatggccgcg gcgttaatcg tctggatgat gttaatcggc caaaaagccc ggaaatcaag
    97621 aacgatattc gtctgttccg ccaggagctg cgtgatgccg cttatcattt ccagggcgat
    97681 gccgacaacg atcagctttc cgtaacgccg ctagtggggc tggggaaatc gagtctgttg
    97741 aacaagacca ttttccatct tatgccttgt gccgagcaga aactaaccat ctgtacgcca
    97801 tacttcaacc tgccagcaat ccttgtgcgc aatattatcc agttgctgcg cgaagggaaa
    97861 aaggtcgaaa ttattgttgg tgataaaacc gcgaacgact tctacattcc ggaagatgaa
    97921 cctttcaaga taattggcgc attgccttat ctctatgaga tcaatctgcg tcgtttcctg
    97981 agccgtttgc agtattacgt caatactgac cagctagtgg ttcggttatg gaaagatgac
    98041 gacaacacct atcacctaaa agggatgtgg gttgatgata agtggatgtt gatcaccggt
    98101 aataacctga acccgcgcgc ctggcgtctg gatctggaaa acgccatttt gatccacgat
    98161 ccacaactgg agcttgcgcc gcagcgagag aaagaactgg agctgatccg cgagcatacc
    98221 accatcgtta agcactatcg cgatctgcaa agtattgccg attatccggt gaaggttcgt
    98281 aaactcatcc gccgtttgcg ccgtatccgc atcgaccgat taattagccg catcctgtaa
    98341 tcacaacccc gtcctgtacg gggtttgttt tttggaggcc acgttttgcg tattcttttt
    98401 gtctgttcgc tattgttact ttctggatgc agccatatgg ctaacgatag ctggagcggg
    98461 caggataaag ctcaacactt tatcgcctcg gcgatgcttt ccgccgccgg aaatgaatat
    98521 tcacagcatc aggggatgag ccgggatcgc agtgccatgt ttggtttgat gttctctgtc
    98581 agtttggggg cgtcaaaaga gttttgggat agccgccccg aagggagcgg ctggagctgg
    98641 aaggatttgg cctgggatgt cgccggtgca agcaccggct ataccgtctg gcaactgacc
    98701 cgtcactaaa gacgcatccc cttccctttg cgatgtagca tcaaagaaac cagaaacgcc
    98761 accacggcca tcaaggtcac ataccagaag aaggctgttt ccattcctat tgatttcagc
    98821 gacaacgcta cgtactccgc cgaaccacca aatatagcat tagcgaccgc atatgacaga
    98881 ccaacgccta atgcgcgaac ctgtgccggg aacatctcag ccttcagtat tccactgatt
    98941 gatgtataaa aactcactat cagcagggca cacatcacca gaccaaaagc ggcataaggc
    99001 gaggaaacgt tttgcaatgc tgagagaata ggaacggtaa aaatggctgc cagcgaaccg
    99061 aaacataaca ttgaggtacg gcgaccaatc ttatccgaca gcgcgccaat gagtggttga
    99121 ataagcatga atacaaacaa tgcggcagtc ataatgccac tcgccacgtt ggcatgcatt
    99181 cccgcagtat ttaccagata cttctgcata taagtggtga aggtatagaa acaaagggag
    99241 cccgcagcgg taaaaccgag aaccatgatg aatgcacggc gattgcgcca taatcctttc
    99301 agagatccag cttcttttaa agcgcgcgtt tcttgttgcg aagtttcatc taactgacga
    99361 cgtaaccaca acgccacaac agctaacaca gctcctaacg cgaaaggaat acgccatccc
    99421 cactctctga gtgcagagtc ttccatggtg tgttgtaaaa ccacgacaac cagtagggct
    99481 agcagttgtc cgccgatcaa cgtcacatac tgaaatgatg cgtaaaaacc tttgcgccct
    99541 tcaacggcaa cttcactcat ataggtggcg ctggtgccat attctccgcc aacggataat
    99601 ccctgaaata aacgcgcgag aagcaataat gccggagccc acgtaccgat tgtttcataa
    99661 ccaggcaaac aggcgattac cagtgagccg aaacacatca tacacaccga tagcagcatc
    99721 gattttttgc gaccatgttt atcggctatg cggccaaata gccaaccgcc tattgggcgc
    99781 atcaggaatc ccgcagcaaa aacacctgct gtttgtagta gttgagtcgt cgtgttcccg
    99841 gaagggaaga agatgtgggc aaagtagagt gaacagaacg agtagacata gaaatcgaac
    99901 cactcgacca gattacctga agaggccccc acaatcgccc aaatgcggcg acgagtatca
    99961 ctacttgtca gtttgctgtc tgccgttaca gtactttcag ccatgccatt atgtctcctg
   100021 ccgtaatccg atgcttttgt cggtcgcttt tgtttatttc ttttggcaaa gaaaatgtta
   100081 tacatttgtt acgtatcatt atgcaacctt aaccatgaat ttagttagca ggaaaatggt
   100141 tattgaggag cttaaggata aatttctggt aaggaggaca cgtatggaag taggcaagtt
   100201 ggggaaaccg tatccgttgc tgaatctggc atatgtggga gtataagacg cgcagcgtcg
   100261 catcaggcat ttttttcacc aacgcaaaaa ggccatcctg acggatggcc ttt
//
LOCUS       DABSYT020000007        98493 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.007, whole genome shotgun sequence.
ACCESSION   DABSYT020000007 DABSYT020000000
VERSION     DABSYT020000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 98493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 98493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.007"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            1..2062
                     /locus_tag="ID725_RS03390"
                     /old_locus_tag="pgaptmp_000678"
                     /pseudo
     CDS             1..2062
                     /locus_tag="ID725_RS03390"
                     /old_locus_tag="pgaptmp_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            2399..3376
                     /gene="glaH"
                     /locus_tag="ID725_RS03395"
                     /old_locus_tag="pgaptmp_000679"
     CDS             2399..3376
                     /gene="glaH"
                     /locus_tag="ID725_RS03395"
                     /old_locus_tag="pgaptmp_000679"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGV7849351.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3396..4664
                     /gene="lhgO"
                     /locus_tag="ID725_RS03400"
                     /old_locus_tag="pgaptmp_000680"
     CDS             3396..4664
                     /gene="lhgO"
                     /locus_tag="ID725_RS03400"
                     /old_locus_tag="pgaptmp_000680"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGV7849352.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4687..6135
                     /gene="gabD"
                     /locus_tag="ID725_RS03405"
                     /old_locus_tag="pgaptmp_000681"
     CDS             4687..6135
                     /gene="gabD"
                     /locus_tag="ID725_RS03405"
                     /old_locus_tag="pgaptmp_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGV7849353.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6149..7429
                     /gene="gabT"
                     /locus_tag="ID725_RS03410"
                     /old_locus_tag="pgaptmp_000682"
     CDS             6149..7429
                     /gene="gabT"
                     /locus_tag="ID725_RS03410"
                     /old_locus_tag="pgaptmp_000682"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGV7849354.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7667..9067
                     /gene="gabP"
                     /locus_tag="ID725_RS03415"
                     /old_locus_tag="pgaptmp_000683"
     CDS             7667..9067
                     /gene="gabP"
                     /locus_tag="ID725_RS03415"
                     /old_locus_tag="pgaptmp_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGV7849355.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9088..9750
                     /gene="csiR"
                     /locus_tag="ID725_RS03420"
                     /old_locus_tag="pgaptmp_000684"
     CDS             9088..9750
                     /gene="csiR"
                     /locus_tag="ID725_RS03420"
                     /old_locus_tag="pgaptmp_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGV7849356.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9751..10200)
                     /gene="kbp"
                     /locus_tag="ID725_RS03425"
                     /old_locus_tag="pgaptmp_000685"
     CDS             complement(9751..10200)
                     /gene="kbp"
                     /locus_tag="ID725_RS03425"
                     /old_locus_tag="pgaptmp_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGV7849357.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10284..10442)
                     /gene="yqaE"
                     /locus_tag="ID725_RS03430"
                     /old_locus_tag="pgaptmp_000686"
     CDS             complement(10284..10442)
                     /gene="yqaE"
                     /locus_tag="ID725_RS03430"
                     /old_locus_tag="pgaptmp_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGV7849358.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10625..10924
                     /gene="ygaV"
                     /locus_tag="ID725_RS03435"
                     /old_locus_tag="pgaptmp_000687"
     CDS             10625..10924
                     /gene="ygaV"
                     /locus_tag="ID725_RS03435"
                     /old_locus_tag="pgaptmp_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGV7849359.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10934..11452
                     /gene="ygaP"
                     /locus_tag="ID725_RS03440"
                     /old_locus_tag="pgaptmp_000688"
     CDS             10934..11452
                     /gene="ygaP"
                     /locus_tag="ID725_RS03440"
                     /old_locus_tag="pgaptmp_000688"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGV7849360.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(11505..11909)
                     /gene="stpA"
                     /locus_tag="ID725_RS03445"
                     /old_locus_tag="pgaptmp_000689"
     CDS             complement(11505..11909)
                     /gene="stpA"
                     /locus_tag="ID725_RS03445"
                     /old_locus_tag="pgaptmp_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGV7849361.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            12577..13026
                     /gene="alaE"
                     /locus_tag="ID725_RS03450"
                     /old_locus_tag="pgaptmp_000690"
     CDS             12577..13026
                     /gene="alaE"
                     /locus_tag="ID725_RS03450"
                     /old_locus_tag="pgaptmp_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGV7849362.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(13063..13407)
                     /gene="ygaC"
                     /locus_tag="ID725_RS03455"
                     /old_locus_tag="pgaptmp_000691"
     CDS             complement(13063..13407)
                     /gene="ygaC"
                     /locus_tag="ID725_RS03455"
                     /old_locus_tag="pgaptmp_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGV7849363.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            13559..13888
                     /gene="ygaM"
                     /locus_tag="ID725_RS03460"
                     /old_locus_tag="pgaptmp_000692"
     CDS             13559..13888
                     /gene="ygaM"
                     /locus_tag="ID725_RS03460"
                     /old_locus_tag="pgaptmp_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGV7849364.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(14038..15372)
                     /locus_tag="ID725_RS03465"
                     /old_locus_tag="pgaptmp_000693"
     CDS             complement(14038..15372)
                     /locus_tag="ID725_RS03465"
                     /old_locus_tag="pgaptmp_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGV7849365.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            15460..15891
                     /locus_tag="ID725_RS03470"
                     /old_locus_tag="pgaptmp_000694"
     CDS             15460..15891
                     /locus_tag="ID725_RS03470"
                     /old_locus_tag="pgaptmp_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGV7849366.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            16100..16345
                     /gene="nrdH"
                     /locus_tag="ID725_RS03475"
                     /old_locus_tag="pgaptmp_000695"
     CDS             16100..16345
                     /gene="nrdH"
                     /locus_tag="ID725_RS03475"
                     /old_locus_tag="pgaptmp_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGV7849367.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            16342..16752
                     /gene="nrdI"
                     /locus_tag="ID725_RS03480"
                     /old_locus_tag="pgaptmp_000696"
     CDS             16342..16752
                     /gene="nrdI"
                     /locus_tag="ID725_RS03480"
                     /old_locus_tag="pgaptmp_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGV7849368.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            16725..18869
                     /gene="nrdE"
                     /locus_tag="ID725_RS03485"
                     /old_locus_tag="pgaptmp_000697"
     CDS             16725..18869
                     /gene="nrdE"
                     /locus_tag="ID725_RS03485"
                     /old_locus_tag="pgaptmp_000697"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGV7849369.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            18879..19838
                     /gene="nrdF"
                     /locus_tag="ID725_RS03490"
                     /old_locus_tag="pgaptmp_000698"
     CDS             18879..19838
                     /gene="nrdF"
                     /locus_tag="ID725_RS03490"
                     /old_locus_tag="pgaptmp_000698"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGV7849370.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
     gene            20194..21396
                     /gene="proV"
                     /locus_tag="ID725_RS03495"
                     /old_locus_tag="pgaptmp_000699"
     CDS             20194..21396
                     /gene="proV"
                     /locus_tag="ID725_RS03495"
                     /old_locus_tag="pgaptmp_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGV7849371.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPNELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            21389..22453
                     /gene="proW"
                     /locus_tag="ID725_RS03500"
                     /old_locus_tag="pgaptmp_000700"
     CDS             21389..22453
                     /gene="proW"
                     /locus_tag="ID725_RS03500"
                     /old_locus_tag="pgaptmp_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGV7849372.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            22510..23502
                     /gene="proX"
                     /locus_tag="ID725_RS03505"
                     /old_locus_tag="pgaptmp_000701"
     CDS             22510..23502
                     /gene="proX"
                     /locus_tag="ID725_RS03505"
                     /old_locus_tag="pgaptmp_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGV7849373.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            23694..24878
                     /locus_tag="ID725_RS03510"
                     /old_locus_tag="pgaptmp_000702"
     CDS             23694..24878
                     /locus_tag="ID725_RS03510"
                     /old_locus_tag="pgaptmp_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7849374.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            25002..25739
                     /gene="ygaZ"
                     /locus_tag="ID725_RS03515"
                     /old_locus_tag="pgaptmp_000703"
     CDS             25002..25739
                     /gene="ygaZ"
                     /locus_tag="ID725_RS03515"
                     /old_locus_tag="pgaptmp_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGV7849375.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            25729..26064
                     /gene="ygaH"
                     /locus_tag="ID725_RS03520"
                     /old_locus_tag="pgaptmp_000704"
     CDS             25729..26064
                     /gene="ygaH"
                     /locus_tag="ID725_RS03520"
                     /old_locus_tag="pgaptmp_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGV7849376.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            26155..26685
                     /gene="emrR"
                     /locus_tag="ID725_RS03525"
                     /old_locus_tag="pgaptmp_000705"
     CDS             26155..26685
                     /gene="emrR"
                     /locus_tag="ID725_RS03525"
                     /old_locus_tag="pgaptmp_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGV7849377.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDHLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            26812..27984
                     /gene="emrA"
                     /locus_tag="ID725_RS03530"
                     /old_locus_tag="pgaptmp_000706"
     CDS             26812..27984
                     /gene="emrA"
                     /locus_tag="ID725_RS03530"
                     /old_locus_tag="pgaptmp_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGV7849378.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            28001..29539
                     /gene="emrB"
                     /locus_tag="ID725_RS03535"
                     /old_locus_tag="pgaptmp_000707"
     CDS             28001..29539
                     /gene="emrB"
                     /locus_tag="ID725_RS03535"
                     /old_locus_tag="pgaptmp_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGV7849379.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(29603..30118)
                     /gene="luxS"
                     /locus_tag="ID725_RS03540"
                     /old_locus_tag="pgaptmp_000708"
     CDS             complement(29603..30118)
                     /gene="luxS"
                     /locus_tag="ID725_RS03540"
                     /old_locus_tag="pgaptmp_000708"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGV7849380.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(30268..31824)
                     /gene="gshA"
                     /locus_tag="ID725_RS03545"
                     /old_locus_tag="pgaptmp_000709"
     CDS             complement(30268..31824)
                     /gene="gshA"
                     /locus_tag="ID725_RS03545"
                     /old_locus_tag="pgaptmp_000709"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGV7849381.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(31897..32325)
                     /gene="yqaA"
                     /locus_tag="ID725_RS03550"
                     /old_locus_tag="pgaptmp_000710"
     CDS             complement(31897..32325)
                     /gene="yqaA"
                     /locus_tag="ID725_RS03550"
                     /old_locus_tag="pgaptmp_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGV7849382.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(32322..32888)
                     /gene="yqaB"
                     /locus_tag="ID725_RS03555"
                     /old_locus_tag="pgaptmp_000711"
     CDS             complement(32322..32888)
                     /gene="yqaB"
                     /locus_tag="ID725_RS03555"
                     /old_locus_tag="pgaptmp_000711"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGV7849383.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(33169..33245)
                     /locus_tag="ID725_RS03560"
                     /old_locus_tag="pgaptmp_000712"
     tRNA            complement(33169..33245)
                     /locus_tag="ID725_RS03560"
                     /old_locus_tag="pgaptmp_000712"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33209..33211),aa:Arg,seq:acg)
     gene            complement(33348..33424)
                     /locus_tag="ID725_RS03565"
                     /old_locus_tag="pgaptmp_000713"
     tRNA            complement(33348..33424)
                     /locus_tag="ID725_RS03565"
                     /old_locus_tag="pgaptmp_000713"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33388..33390),aa:Arg,seq:acg)
     gene            complement(33487..33563)
                     /locus_tag="ID725_RS03570"
                     /old_locus_tag="pgaptmp_000714"
     tRNA            complement(33487..33563)
                     /locus_tag="ID725_RS03570"
                     /old_locus_tag="pgaptmp_000714"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33527..33529),aa:Arg,seq:acg)
     gene            complement(33628..33704)
                     /locus_tag="ID725_RS03575"
                     /old_locus_tag="pgaptmp_000715"
     tRNA            complement(33628..33704)
                     /locus_tag="ID725_RS03575"
                     /old_locus_tag="pgaptmp_000715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33668..33670),aa:Arg,seq:acg)
     gene            complement(33708..33800)
                     /locus_tag="ID725_RS03580"
                     /old_locus_tag="pgaptmp_000716"
     tRNA            complement(33708..33800)
                     /locus_tag="ID725_RS03580"
                     /old_locus_tag="pgaptmp_000716"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33764..33766),aa:Ser,seq:gct)
     gene            complement(34116..34301)
                     /gene="csrA"
                     /locus_tag="ID725_RS03585"
                     /old_locus_tag="pgaptmp_000717"
     CDS             complement(34116..34301)
                     /gene="csrA"
                     /locus_tag="ID725_RS03585"
                     /old_locus_tag="pgaptmp_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGV7849384.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(34536..37166)
                     /gene="alaS"
                     /locus_tag="ID725_RS03590"
                     /old_locus_tag="pgaptmp_000718"
     CDS             complement(34536..37166)
                     /gene="alaS"
                     /locus_tag="ID725_RS03590"
                     /old_locus_tag="pgaptmp_000718"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGV7849385.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(37294..37794)
                     /gene="recX"
                     /locus_tag="ID725_RS03595"
                     /old_locus_tag="pgaptmp_000719"
     CDS             complement(37294..37794)
                     /gene="recX"
                     /locus_tag="ID725_RS03595"
                     /old_locus_tag="pgaptmp_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGV7849386.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(37862..38923)
                     /gene="recA"
                     /locus_tag="ID725_RS03600"
                     /old_locus_tag="pgaptmp_000720"
     CDS             complement(37862..38923)
                     /gene="recA"
                     /locus_tag="ID725_RS03600"
                     /old_locus_tag="pgaptmp_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGV7849387.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(39003..39500)
                     /gene="pncC"
                     /locus_tag="ID725_RS03605"
                     /old_locus_tag="pgaptmp_000721"
     CDS             complement(39003..39500)
                     /gene="pncC"
                     /locus_tag="ID725_RS03605"
                     /old_locus_tag="pgaptmp_000721"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGV7849388.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(39645..40730)
                     /gene="mltB"
                     /locus_tag="ID725_RS03610"
                     /old_locus_tag="pgaptmp_000722"
     CDS             complement(39645..40730)
                     /gene="mltB"
                     /locus_tag="ID725_RS03610"
                     /old_locus_tag="pgaptmp_000722"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGV7849389.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            40985..41547
                     /gene="srlA"
                     /locus_tag="ID725_RS03615"
                     /old_locus_tag="pgaptmp_000723"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             40985..41547
                     /gene="srlA"
                     /locus_tag="ID725_RS03615"
                     /old_locus_tag="pgaptmp_000723"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            41544..42505
                     /gene="srlE"
                     /locus_tag="ID725_RS03620"
                     /old_locus_tag="pgaptmp_000724"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             41544..42505
                     /gene="srlE"
                     /locus_tag="ID725_RS03620"
                     /old_locus_tag="pgaptmp_000724"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            42516..42887
                     /gene="srlB"
                     /locus_tag="ID725_RS03625"
                     /old_locus_tag="pgaptmp_000725"
                     /gene_synonym="gutB"
     CDS             42516..42887
                     /gene="srlB"
                     /locus_tag="ID725_RS03625"
                     /old_locus_tag="pgaptmp_000725"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGV7849390.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            42891..43670
                     /gene="srlD"
                     /locus_tag="ID725_RS03630"
                     /old_locus_tag="pgaptmp_000726"
     CDS             42891..43670
                     /gene="srlD"
                     /locus_tag="ID725_RS03630"
                     /old_locus_tag="pgaptmp_000726"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGV7849391.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            43776..44135
                     /gene="gutM"
                     /locus_tag="ID725_RS03635"
                     /old_locus_tag="pgaptmp_000727"
     CDS             43776..44135
                     /gene="gutM"
                     /locus_tag="ID725_RS03635"
                     /old_locus_tag="pgaptmp_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGV7849392.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            44202..44975
                     /gene="srlR"
                     /locus_tag="ID725_RS03640"
                     /old_locus_tag="pgaptmp_000728"
                     /gene_synonym="gutR"
     CDS             44202..44975
                     /gene="srlR"
                     /locus_tag="ID725_RS03640"
                     /old_locus_tag="pgaptmp_000728"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGV7849393.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            44968..45933
                     /gene="gutQ"
                     /locus_tag="ID725_RS03645"
                     /old_locus_tag="pgaptmp_000729"
     CDS             44968..45933
                     /gene="gutQ"
                     /locus_tag="ID725_RS03645"
                     /old_locus_tag="pgaptmp_000729"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGV7849394.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(45930..47444)
                     /gene="norR"
                     /locus_tag="ID725_RS03650"
                     /old_locus_tag="pgaptmp_000730"
     CDS             complement(45930..47444)
                     /gene="norR"
                     /locus_tag="ID725_RS03650"
                     /old_locus_tag="pgaptmp_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGV7849395.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            47631..49070
                     /gene="norV"
                     /locus_tag="ID725_RS03655"
                     /old_locus_tag="pgaptmp_000731"
     CDS             47631..49070
                     /gene="norV"
                     /locus_tag="ID725_RS03655"
                     /old_locus_tag="pgaptmp_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGV7849396.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEKMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            49067..50200
                     /gene="norW"
                     /locus_tag="ID725_RS03660"
                     /old_locus_tag="pgaptmp_000732"
     CDS             49067..50200
                     /gene="norW"
                     /locus_tag="ID725_RS03660"
                     /old_locus_tag="pgaptmp_000732"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGV7849397.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(50328..52580)
                     /gene="hypF"
                     /locus_tag="ID725_RS03665"
                     /old_locus_tag="pgaptmp_000733"
     CDS             complement(50328..52580)
                     /gene="hypF"
                     /locus_tag="ID725_RS03665"
                     /old_locus_tag="pgaptmp_000733"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGV7849398.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(52733..53260)
                     /gene="hydN"
                     /locus_tag="ID725_RS03670"
                     /old_locus_tag="pgaptmp_000734"
     CDS             complement(52733..53260)
                     /gene="hydN"
                     /locus_tag="ID725_RS03670"
                     /old_locus_tag="pgaptmp_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGV7849399.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(53409..54419)
                     /gene="ascG"
                     /locus_tag="ID725_RS03675"
                     /old_locus_tag="pgaptmp_000735"
     CDS             complement(53409..54419)
                     /gene="ascG"
                     /locus_tag="ID725_RS03675"
                     /old_locus_tag="pgaptmp_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGV7849400.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            54679..56136
                     /gene="ascF"
                     /locus_tag="ID725_RS03680"
                     /old_locus_tag="pgaptmp_000736"
     CDS             54679..56136
                     /gene="ascF"
                     /locus_tag="ID725_RS03680"
                     /old_locus_tag="pgaptmp_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGV7849401.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNSELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            56145..57569
                     /gene="ascB"
                     /locus_tag="ID725_RS03685"
                     /old_locus_tag="pgaptmp_000737"
     CDS             56145..57569
                     /gene="ascB"
                     /locus_tag="ID725_RS03685"
                     /old_locus_tag="pgaptmp_000737"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGV7849402.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(57693..58163)
                     /gene="hycI"
                     /locus_tag="ID725_RS03690"
                     /old_locus_tag="pgaptmp_000738"
     CDS             complement(57693..58163)
                     /gene="hycI"
                     /locus_tag="ID725_RS03690"
                     /old_locus_tag="pgaptmp_000738"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGV7849403.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(58156..58566)
                     /gene="hycH"
                     /locus_tag="ID725_RS03695"
                     /old_locus_tag="pgaptmp_000739"
     CDS             complement(58156..58566)
                     /gene="hycH"
                     /locus_tag="ID725_RS03695"
                     /old_locus_tag="pgaptmp_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGV7849404.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(58563..59330)
                     /gene="hycG"
                     /locus_tag="ID725_RS03700"
                     /old_locus_tag="pgaptmp_000740"
     CDS             complement(58563..59330)
                     /gene="hycG"
                     /locus_tag="ID725_RS03700"
                     /old_locus_tag="pgaptmp_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGV7849405.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(59330..59872)
                     /gene="hycF"
                     /locus_tag="ID725_RS03705"
                     /old_locus_tag="pgaptmp_000741"
     CDS             complement(59330..59872)
                     /gene="hycF"
                     /locus_tag="ID725_RS03705"
                     /old_locus_tag="pgaptmp_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGV7849406.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(59882..61591)
                     /gene="hycE"
                     /locus_tag="ID725_RS03710"
                     /old_locus_tag="pgaptmp_000742"
     CDS             complement(59882..61591)
                     /gene="hycE"
                     /locus_tag="ID725_RS03710"
                     /old_locus_tag="pgaptmp_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGV7849407.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(61609..62532)
                     /gene="hycD"
                     /locus_tag="ID725_RS03715"
                     /old_locus_tag="pgaptmp_000743"
     CDS             complement(61609..62532)
                     /gene="hycD"
                     /locus_tag="ID725_RS03715"
                     /old_locus_tag="pgaptmp_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGV7849408.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(62535..64361)
                     /gene="hycC"
                     /locus_tag="ID725_RS03720"
                     /old_locus_tag="pgaptmp_000744"
     CDS             complement(62535..64361)
                     /gene="hycC"
                     /locus_tag="ID725_RS03720"
                     /old_locus_tag="pgaptmp_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGV7849409.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(64358..64969)
                     /gene="hycB"
                     /locus_tag="ID725_RS03725"
                     /old_locus_tag="pgaptmp_000745"
     CDS             complement(64358..64969)
                     /gene="hycB"
                     /locus_tag="ID725_RS03725"
                     /old_locus_tag="pgaptmp_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGV7849410.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(65094..65555)
                     /gene="hycA"
                     /locus_tag="ID725_RS03730"
                     /old_locus_tag="pgaptmp_000746"
     CDS             complement(65094..65555)
                     /gene="hycA"
                     /locus_tag="ID725_RS03730"
                     /old_locus_tag="pgaptmp_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGV7849411.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            65767..66117
                     /gene="hypA"
                     /locus_tag="ID725_RS03735"
                     /old_locus_tag="pgaptmp_000747"
     CDS             65767..66117
                     /gene="hypA"
                     /locus_tag="ID725_RS03735"
                     /old_locus_tag="pgaptmp_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGV7849412.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            66121..66993
                     /gene="hypB"
                     /locus_tag="ID725_RS03740"
                     /old_locus_tag="pgaptmp_000748"
     CDS             66121..66993
                     /gene="hypB"
                     /locus_tag="ID725_RS03740"
                     /old_locus_tag="pgaptmp_000748"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGV7849413.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            66984..67256
                     /gene="hypC"
                     /locus_tag="ID725_RS03745"
                     /old_locus_tag="pgaptmp_000749"
     CDS             66984..67256
                     /gene="hypC"
                     /locus_tag="ID725_RS03745"
                     /old_locus_tag="pgaptmp_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGV7849414.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            67256..68377
                     /gene="hypD"
                     /locus_tag="ID725_RS03750"
                     /old_locus_tag="pgaptmp_000750"
     CDS             67256..68377
                     /gene="hypD"
                     /locus_tag="ID725_RS03750"
                     /old_locus_tag="pgaptmp_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGV7849415.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            68374..69384
                     /gene="hypE"
                     /locus_tag="ID725_RS03755"
                     /old_locus_tag="pgaptmp_000751"
     CDS             68374..69384
                     /gene="hypE"
                     /locus_tag="ID725_RS03755"
                     /old_locus_tag="pgaptmp_000751"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGV7849416.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            69458..71536
                     /gene="flhA"
                     /locus_tag="ID725_RS03760"
                     /old_locus_tag="pgaptmp_000752"
     CDS             69458..71536
                     /gene="flhA"
                     /locus_tag="ID725_RS03760"
                     /old_locus_tag="pgaptmp_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGV7849417.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(71573..71926)
                     /gene="ygbA"
                     /locus_tag="ID725_RS03765"
                     /old_locus_tag="pgaptmp_000753"
     CDS             complement(71573..71926)
                     /gene="ygbA"
                     /locus_tag="ID725_RS03765"
                     /old_locus_tag="pgaptmp_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGV7849418.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(72003..72137)
                     /locus_tag="ID725_RS03770"
                     /old_locus_tag="pgaptmp_000754"
     CDS             complement(72003..72137)
                     /locus_tag="ID725_RS03770"
                     /old_locus_tag="pgaptmp_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849419.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            72213..74774
                     /gene="mutS"
                     /locus_tag="ID725_RS03775"
                     /old_locus_tag="pgaptmp_000755"
     CDS             72213..74774
                     /gene="mutS"
                     /locus_tag="ID725_RS03775"
                     /old_locus_tag="pgaptmp_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGV7849420.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            74880..75536
                     /gene="pphB"
                     /locus_tag="ID725_RS03780"
                     /old_locus_tag="pgaptmp_000756"
     CDS             74880..75536
                     /gene="pphB"
                     /locus_tag="ID725_RS03780"
                     /old_locus_tag="pgaptmp_000756"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGV7849421.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(75577..75813)
                     /locus_tag="ID725_RS03785"
                     /old_locus_tag="pgaptmp_000757"
     CDS             complement(75577..75813)
                     /locus_tag="ID725_RS03785"
                     /old_locus_tag="pgaptmp_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGV7849422.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(75824..77251)
                     /locus_tag="ID725_RS03790"
                     /old_locus_tag="pgaptmp_000758"
     CDS             complement(75824..77251)
                     /locus_tag="ID725_RS03790"
                     /old_locus_tag="pgaptmp_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGV7849423.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(77251..77844)
                     /locus_tag="ID725_RS03795"
                     /old_locus_tag="pgaptmp_000759"
     CDS             complement(77251..77844)
                     /locus_tag="ID725_RS03795"
                     /old_locus_tag="pgaptmp_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGV7849424.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            77991..78398
                     /locus_tag="ID725_RS03800"
                     /old_locus_tag="pgaptmp_000760"
     CDS             77991..78398
                     /locus_tag="ID725_RS03800"
                     /old_locus_tag="pgaptmp_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGV7849425.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(78518..79510)
                     /gene="rpoS"
                     /locus_tag="ID725_RS03805"
                     /old_locus_tag="pgaptmp_000761"
     CDS             complement(78518..79510)
                     /gene="rpoS"
                     /locus_tag="ID725_RS03805"
                     /old_locus_tag="pgaptmp_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HGV7849426.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(79573..80619)
                     /gene="nlpD"
                     /locus_tag="ID725_RS03810"
                     /old_locus_tag="pgaptmp_000762"
     CDS             complement(79573..80619)
                     /gene="nlpD"
                     /locus_tag="ID725_RS03810"
                     /old_locus_tag="pgaptmp_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGV7849427.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFY
                     IAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVI
                     KPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAPVTVPTASTTEPTVS
                     STSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNAL
                     RGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRY
                     KGKSVNPLRYLPQR"
     gene            complement(80804..81430)
                     /gene="pcm"
                     /locus_tag="ID725_RS03815"
                     /old_locus_tag="pgaptmp_000763"
     CDS             complement(80804..81430)
                     /gene="pcm"
                     /locus_tag="ID725_RS03815"
                     /old_locus_tag="pgaptmp_000763"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGV7849428.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(81424..82185)
                     /gene="surE"
                     /locus_tag="ID725_RS03820"
                     /old_locus_tag="pgaptmp_000764"
     CDS             complement(81424..82185)
                     /gene="surE"
                     /locus_tag="ID725_RS03820"
                     /old_locus_tag="pgaptmp_000764"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGV7849429.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(82166..83215)
                     /gene="truD"
                     /locus_tag="ID725_RS03825"
                     /old_locus_tag="pgaptmp_000765"
     CDS             complement(82166..83215)
                     /gene="truD"
                     /locus_tag="ID725_RS03825"
                     /old_locus_tag="pgaptmp_000765"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGV7849430.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(83212..83691)
                     /gene="ispF"
                     /locus_tag="ID725_RS03830"
                     /old_locus_tag="pgaptmp_000766"
     CDS             complement(83212..83691)
                     /gene="ispF"
                     /locus_tag="ID725_RS03830"
                     /old_locus_tag="pgaptmp_000766"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGV7849431.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(83691..84401)
                     /gene="ispD"
                     /locus_tag="ID725_RS03835"
                     /old_locus_tag="pgaptmp_000767"
     CDS             complement(83691..84401)
                     /gene="ispD"
                     /locus_tag="ID725_RS03835"
                     /old_locus_tag="pgaptmp_000767"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGV7849432.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(84420..84731)
                     /gene="ftsB"
                     /locus_tag="ID725_RS03840"
                     /old_locus_tag="pgaptmp_000768"
     CDS             complement(84420..84731)
                     /gene="ftsB"
                     /locus_tag="ID725_RS03840"
                     /old_locus_tag="pgaptmp_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGV7849433.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(84925..85248)
                     /gene="ygbE"
                     /locus_tag="ID725_RS03845"
                     /old_locus_tag="pgaptmp_000769"
     CDS             complement(84925..85248)
                     /gene="ygbE"
                     /locus_tag="ID725_RS03845"
                     /old_locus_tag="pgaptmp_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGV7849434.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(85298..85903)
                     /gene="cysC"
                     /locus_tag="ID725_RS03850"
                     /old_locus_tag="pgaptmp_000770"
     CDS             complement(85298..85903)
                     /gene="cysC"
                     /locus_tag="ID725_RS03850"
                     /old_locus_tag="pgaptmp_000770"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGV7849435.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(85903..87330)
                     /gene="cysN"
                     /locus_tag="ID725_RS03855"
                     /old_locus_tag="pgaptmp_000771"
     CDS             complement(85903..87330)
                     /gene="cysN"
                     /locus_tag="ID725_RS03855"
                     /old_locus_tag="pgaptmp_000771"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGV7849436.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(87332..88240)
                     /gene="cysD"
                     /locus_tag="ID725_RS03860"
                     /old_locus_tag="pgaptmp_000772"
     CDS             complement(87332..88240)
                     /gene="cysD"
                     /locus_tag="ID725_RS03860"
                     /old_locus_tag="pgaptmp_000772"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGV7849437.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            88492..89529
                     /gene="iap"
                     /locus_tag="ID725_RS03865"
                     /old_locus_tag="pgaptmp_000773"
     CDS             88492..89529
                     /gene="iap"
                     /locus_tag="ID725_RS03865"
                     /old_locus_tag="pgaptmp_000773"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGV7849438.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   89611..89823
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=89672..89701
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(89919..90212)
                     /gene="cas2e"
                     /locus_tag="ID725_RS03870"
                     /old_locus_tag="pgaptmp_000774"
     CDS             complement(89919..90212)
                     /gene="cas2e"
                     /locus_tag="ID725_RS03870"
                     /old_locus_tag="pgaptmp_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGV7849439.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(90209..91132)
                     /gene="cas1e"
                     /locus_tag="ID725_RS03875"
                     /old_locus_tag="pgaptmp_000775"
     CDS             complement(90209..91132)
                     /gene="cas1e"
                     /locus_tag="ID725_RS03875"
                     /old_locus_tag="pgaptmp_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGV7849440.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(91129..91779)
                     /gene="cas6e"
                     /locus_tag="ID725_RS03880"
                     /old_locus_tag="pgaptmp_000776"
     CDS             complement(91129..91779)
                     /gene="cas6e"
                     /locus_tag="ID725_RS03880"
                     /old_locus_tag="pgaptmp_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGV7849441.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(91761..92507)
                     /gene="cas5e"
                     /locus_tag="ID725_RS03885"
                     /old_locus_tag="pgaptmp_000777"
     CDS             complement(91761..92507)
                     /gene="cas5e"
                     /locus_tag="ID725_RS03885"
                     /old_locus_tag="pgaptmp_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGV7849442.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(92518..93573)
                     /gene="cas7e"
                     /locus_tag="ID725_RS03890"
                     /old_locus_tag="pgaptmp_000778"
     CDS             complement(92518..93573)
                     /gene="cas7e"
                     /locus_tag="ID725_RS03890"
                     /old_locus_tag="pgaptmp_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGV7849443.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(93585..94121)
                     /gene="casB"
                     /locus_tag="ID725_RS03895"
                     /old_locus_tag="pgaptmp_000779"
                     /gene_synonym="cse2"
     CDS             complement(93585..94121)
                     /gene="casB"
                     /locus_tag="ID725_RS03895"
                     /old_locus_tag="pgaptmp_000779"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGV7849444.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(94118..95680)
                     /gene="casA"
                     /locus_tag="ID725_RS03900"
                     /old_locus_tag="pgaptmp_000780"
                     /gene_synonym="cse1"
     CDS             complement(94118..95680)
                     /gene="casA"
                     /locus_tag="ID725_RS03900"
                     /old_locus_tag="pgaptmp_000780"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGV7849445.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(95778..98477)
                     /locus_tag="ID725_RS03905"
                     /old_locus_tag="pgaptmp_000781"
     CDS             complement(95778..98477)
                     /locus_tag="ID725_RS03905"
                     /old_locus_tag="pgaptmp_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HGV7849446.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAP
                     ELKIPEGSRKNYEKISHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITT
                     GHHGRPPDRIDELDNFLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGI
                     NTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKE
                     DGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRQWCIEQRQDPEMANVILVTDDESCGYSAREGLIGKVD"
BASE COUNT        23520 a        26433 c        25187 g        23353 t
ORIGIN      
        1 tcacgcagaa aaagtcatta atgatctgtt aaaaaacgat tataaaattt ccctttctcc
       61 agatacaact taccgagagc tgaataaagc tgcacagcgt agcattacag cgccagacag
      121 gataggagaa gggaaaatat gggtttatca acgagataca atggttgaaa gaggtgataa
      181 cagcggtgtt catcagtatg gtccagctga acatttcact cacattatat ctgacaaacc
      241 gtccccaaaa gataagtatg ttgcatatgc tattaacatt cctgactatg agctagcagc
      301 cgatgtatat aatattaacg tgacgtcacc ttccggacag caagaaacat ttaaaatatt
      361 aatcaatcca gaacatctac ggcaaacact tgagcgtaaa tcccttactg ctgttcagaa
      421 atcacaatgt gaaatcatca cccccaaaaa acctggcgaa gcgattcttc atgcttttaa
      481 tgccacctac cagcaaatca gggaaaatat gtctgagttt gcacgttgcc attatgggta
      541 tatacaaatc cctccagtga caactttccg cgccgacgga ccagaaactc ccgaagaaga
      601 aaaaggttac tggtttcacg cttatcaacc cgaagatctt tgtaccatcc ataatccaat
      661 gggagatttg caggatttta tcgcattggt taaagatgct aaaaaatttg gtatcgatat
      721 cattcctgat tataccttta actttatggg aatcgggggt agtggtaaaa atgacctgga
      781 ttatccctct gctgatatac gagcgaagat cagtaaagat atagaaggtg gtatccctgg
      841 ctattggcaa ggtcaggttt tgattccatt tactatagat ccagtaacaa aagaacgtaa
      901 acaaatccat ccagaagata tacatctcac agcaaaagac ttcgaagcaa gtaaagataa
      961 tatttctaag gatgaatggg aaaacctcca tgcattaaaa gaaaagcgtt taaatggaat
     1021 gcctaaaacc ccccccaaaa gtgactaggt tattatgttg caaaatcaat acgttcgtga
     1081 aatgcgaaaa tatggcgtac gaggtttacg ttacgatgca gcaaaacact caaaacatga
     1141 acaaatagaa agatcaataa ccccaccgct taaaaattat aatgagcgat tacacaatac
     1201 taacttattt aacccaaaat atcatgaaaa agccgttatg aattacatgg aatatctggt
     1261 aacttgccag ttggatgaag aacaaatgtc atcgctgctt tatgaaagag atgatttaag
     1321 cgccattgat ttttcattgc tcatgaagac gataaaagct ttttcatttg gtggcgatct
     1381 ccaaaccctt gcatcaaaac cgagttccac aatctcaagc atcccatcaa aaagacggat
     1441 attgattaac attaaccacg attttcctaa caatggcaat cttttcaatg actttctatt
     1501 taaccatcaa caagatgaac aattagcaat ggcatatatg gccgctctcc cgttcagcag
     1561 gcctttagtt tactgggatg gccaagtatt aaaatcaacg actgaaatta aaaattatga
     1621 tgggtcgacg cgtgtcggcg gtgaggcgtg gcttaataaa ggttgctcta cctatcagca
     1681 gctctacaat gaattccacg cattatatat agataaagca ggaatatgga gcgcatttga
     1741 gggtgtattt gcaactaaaa acgttctggc ctttagtcgt ggggattcgg tgaacattaa
     1801 tcactctcct catgatggac tagttataat aaataaagga aacgaagaag ttgaaggtgc
     1861 ctggcctaac aaattgcaac ctggaaaata taaaaacatg gggagtaata gcgttaacat
     1921 tattattaat aatacccgaa aaattatccc ccctggtaaa gcatttatgc ttagaggcgg
     1981 aactctaaat atcaatattc ctgggcgtag cgctcttctt ttagggaaaa caggagaacc
     2041 gctgaactat ctctatttgt aatttaatgt tatatctgcc ccgctaaaac ggggcagata
     2101 atatatttag tttactagcg gtcattttgc agtgaagcca tttaccgttt tttatcgacc
     2161 agataatctg ttctctgatg gtaatccccc cctaacctgt tgctttagtt attcatttcc
     2221 tgtctcactt tgccttaata ccctacgtta aatgttacca atttgttgct ttttatcaca
     2281 ataagaaaac aatatggcgc atttgtgcgc atttttcaga aatgtagata tttttagatt
     2341 atggctacga aatgagcatc gccatgtcac cctacatctc ataagaggat cgcttctgat
     2401 gaatgcactg accgccgtac aaaataacgc tgtcgattca gaccaggact atagcggatt
     2461 cactctcatc ccgtcggcgc aatccccgcg tctgctggaa ctcaccttca ccgaacagac
     2521 gaccaaacag tttctcgaac aggttgccga atggcccgtg caggcgctgg agtacaaatc
     2581 gtttctgcgt tttcgggtag gtaaaattct tgacgatctg tgtgcgaacc agctgcaacc
     2641 attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg ctgttgatca atgcggtggg
     2701 tgtcgatgat gtgaagcaag cggatgagat ggtgaagctg gcgacggcgg tggcgcatct
     2761 gattggccgt tccaatttcg atgcgatgag cggtcagtat tacgcgcgtt ttgtagtgaa
     2821 aaatgtcgat aactcagaca gctatctgcg tcagccgcac cgcgtaatgg agctgcacaa
     2881 cgacggcacc tacgtcgaag agatcactga ctatgtgctg atgatgaaaa tcgacgagca
     2941 gaacatgcag ggtggaaatt cgttgctgct gcatctcgat gactgggaac atctggacca
     3001 ctatttccgc catccaatgg cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa
     3061 cgtcagcaaa gatgttttcc atccagtgtt cgacgttgat caacagggtc gcccggtgat
     3121 gcgctatatc gaccagttcg tccagccaaa agacttcgaa gaaggcgtgt ggttgagcga
     3181 gctttcggac gccattgaaa tcagtaaagg cattctttct gtacccgttc ccgttggcaa
     3241 attcctgttg attaacaacc tgttctggct gcacggtcgc gaccgcttta ctccgcaccc
     3301 ggatctgcgc cgtgaactga tgcgtcagcg tggctatttc gcttacgcca ctcaccacta
     3361 tcagacgcat cagtaagcgc aaaggaattg agcggatgta tgattttgtg attattggcg
     3421 gcggcatcat cggcatgtcg accgccatgc aactgattga tgtctatccg gatgcccgca
     3481 ttgcgttgct ggaaaaagag tccggcccgg cctgtcatca gacgggccac aacagcggcg
     3541 tgatccatgc cggggtttat tacacgcccg gtagcctgaa ggcgcagttt tgcctggcgg
     3601 gaaaccgcgc cactaaagcc ttttgcgatc aaaacggcat tcgctatgac aactgcggca
     3661 agatgctggt cgccacctct gaactcgaaa tggaacggat gcgcgcgttg tgggaacgca
     3721 cggcggcaaa cggtatcgag cgcgagtggt taaacgccga tgaactgcgc gagcgcgaac
     3781 cgaatatcac cgggcttggc ggtatttttg tgccgtccag cggcattgtc agctaccgcg
     3841 aagtcacggc ggcgatggca aaaattttcc aggccagagg cggcgagatt atctataacg
     3901 ccgaagtcag cggccttagt gagcataaaa gcggcgtggt gatacgtacc cgtcagggca
     3961 gcgactatga agcatcgacg ctgattagct gttccgggct gatggctgac cggctggtga
     4021 aaatgctcgg cctcgaaccg ggctttatta tctgcccgtt ccgcggcgag tatttccgcc
     4081 tggcgccgga gcataaccag attgttaacc atctgattta ccccattccc gacccggcaa
     4141 tgccgttttt gggcgtgcat ctcacccgca tgatcgacgg cagcgtcacc gtcgggccaa
     4201 acgcggtgct ggctttcaaa cgcgaaggct atcgcaagcg cgacttctca tttagcgaca
     4261 cgctggagat tttgggctcg tcggggattc gccgggtgct gcaaaaccat ctacgctcag
     4321 gactgggcga gatgaaaaac tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt
     4381 attgtccccg gctttcgtta agcgatctcc agccctggcc cgccggtgtg cgggcgcagg
     4441 cggtatcgcc ggacggcaag ctgattgacg attttctgtt tgtcaccacc ccgcgcacga
     4501 tccacacctg caatgcgccc tccccggcag cgacatcagc aattcctatt ggtgcgcata
     4561 ttgtcagcaa ggtacaaacg ctgttggcaa gccagagtaa ccccggacgc acgctgcgag
     4621 cggcacgtag tgtggatgcc ttacacgccg cgtttaatca ataacctttg aaaacaggat
     4681 gtagcgatga aactgaacga cagtaactta ttccgccagc aggcgttgat taacggggaa
     4741 tggctggacg ccaacaatgg tgaagtcatc gacgtcacca atccggcgaa cggcgacaag
     4801 ctgggtagcg tgccgaaaat gggcgctgat gaaactcgcg ccgctatcga cgccgccaac
     4861 cgcgccctgc ccgtctggcg tgcgctcacc gccaaagaac gcgccaacat tctgcgcaac
     4921 tggttcaatt tgatgatgga gcatcaggac gatttagcgc gcctgatgac cctcgaacag
     4981 ggtaaaccgc tggctgaagc gaaaggcgaa atcagctacg ccgcctcttt tattgagtgg
     5041 tttgccgaag aaggcaaacg catttatggc gacaccattc ccggtcatca ggccgataag
     5101 cgcctgattg tgatcaagca gccgattggc gttacagcgg ccatcacgcc gtggaacttt
     5161 ccggcggcaa tgattacccg caaagccggt ccggcgctgg cggcaggctg caccatggtg
     5221 ctgaagcccg ccagtcagac gccgttctct gcgctggcgc tggcggagct ggcgatccgc
     5281 gcgggcattc cggctggcgt gtttaacgtg gtcaccggtt cggcgggcgc ggtgggtaat
     5341 gaactgacca gcaacccgct ggtacgcaaa ctgtcgttta ccggctcgac cgaaattggc
     5401 cgccagttaa tggaacagtg cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc
     5461 aacgcgccgt ttatcgtctt tgacgatgcc gatctcgata aagccgtgga aggcgcactg
     5521 gcctcgaaat tccgcaacgc cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag
     5581 gacggcgtgt atgaccgctt tgccgaaaaa ttgcagcagg cggtgagcaa actgcatatc
     5641 ggcgacgggc tggataaagg cgtcaccatc gggccgctga tcgatgaaaa agcggtagca
     5701 aaagtggaag agcatattgc cgatgcgctg gaaaaaggcg cgcgcgtagt ttgcggcggt
     5761 aaagcacatg aacgcggcgg caacttcttc cagcccacca ttctggtgga cgtgccggcc
     5821 aacgccaaag tgtcgaaaga agagaccttc ggccccctcg ccccgctgtt ccgttttaaa
     5881 gatgaagccg atgtgatcgc gcaagccaat gacaccgagt ttggccttgc cgcctatttc
     5941 tatgcccgtg atttaagtcg cgtcttccgc gtgggcgagg cgctggagta cggcatcgtc
     6001 ggcatcaata ccgggattat ttccaatgaa gtggccccgt tcggcggcat taaagcctcg
     6061 ggtctgggtc gtgaaggttc gaagtatggc atcgaagatt acttagaaat caaatatatg
     6121 tgcatcggtc tttaactgga gaacgcgaat gagcagcaat aaagagttaa tgcagcgccg
     6181 cagtcaggca attcctcgtg gcgttgggca aattcacccc attttcgctg atcgcgcgga
     6241 aaactgccgg gtgtgggacg ttgaaggccg tgagtatctt gatttcgcgg gcggcattgc
     6301 ggtgctcaat accgggcacc tgcatccgaa agtggttgcc gcggtggaag cgcagttgaa
     6361 aaaactgtcg cacacctgct tccaggtgct ggcttacgag ccgtatctgg agctgtgcga
     6421 gattatgaat cagaaggtgc cgggcgattt tgccaagaaa acgctgctgg ttacgaccgg
     6481 ttccgaagcg gtggaaaacg cggtgaaaat cgcccgcgct gccaccaaac gtagcggcac
     6541 catcgctttt agcggcgcgt atcacgggcg cacgcattac acgctggcgc tgaccggcaa
     6601 ggtgaatccg tactctgcgg gcatgggcct gatgccaggg cacgtttatc gcgcgcttta
     6661 tccttgccca ctgcacggca tcagtgaaga tgatgctatc gccagtatcc accgaatttt
     6721 taaaaatgat gctgcgccgg aagatatcgc cgccatcgtg attgagccgg ttcagggcga
     6781 aggcggtttc tacgccgcga cgcctgcgtt tatgcagcgt ttacgcgcgc tgtgtgacga
     6841 gcacgggatc atgctgattg ccgatgaagt gcagagcggc gcggggcgta ccggcacgct
     6901 gtttgcgatg gagcaaatgg gcgtggcacc agatctcacc acctttgcga aatcgatcgc
     6961 aggcggcttc ccactggcgg gcgtcaccgg gcgcgccgaa gtgatggatg ccgtcgctcc
     7021 aggcgggctg ggtggcacct atgccggtaa tccgattgcc tgcgtggcgg cgctggaagt
     7081 gttgaaggtg ttcgagcagg aaaatctgct gcagaaagcc aacgatctgg ggcagaagtt
     7141 gaaagatgga ttgttggcga tcgccgaaaa acaccctgag atcggcgacg tacgcgggct
     7201 gggggcgatg atcgccatcg agctgtttga agacggcgat cacaacaagc cggacgccaa
     7261 actcaccgcc gagatcgtgg ctcgcgcccg cgataaaggc ctgattcttc tctcctgcgg
     7321 cccgtattac aacgtgctgc gcatccttgt accgctcacc attgaagacg ctcagatccg
     7381 tcagggtctg gagatcatca gccagtgttt tgctgaggca aagcagtagc gccgctccta
     7441 tgccggaggc gacgctgcgc gtcttgtccg gcctacgggg atccaggtcg gataaggcgt
     7501 ttacgccgca tccgacaatc tgtacgtgaa caggaataaa tctatgttgg ccgggtaagg
     7561 cggagccgct ctccggcaaa aagaatcaat aacaattata cgcgtgaccc gacgcgggaa
     7621 atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg ggcaatcatc
     7681 gcaaccacat gagttaggcg gcgggctgaa gtcacgccat gtcaccatgt tgtctattgc
     7741 cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg aagcgggccc
     7801 ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta tgcggatgtt
     7861 ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg ccgataaagc
     7921 tattggtcgt tgggcgggtt ataccatcgg ctggttgtac tggtggtttt gggtactggt
     7981 tatcccgctg gaagccaaca tcgccgccat gatcctgcac tcatgggttc caggcattcc
     8041 catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt tattaagcgt
     8101 taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg ctatcctggc
     8161 ctttattttc cttggtgcag tcgcaattag cggtttttac ccgtatgccg acgtgagcgg
     8221 gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggcttcggtg cggtattaag
     8281 cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca ttgccgccgc
     8341 ggaatccgac acgccggaaa aacatattgt ccgcgctacc aactcggtta tctggcgtat
     8401 ttctatcttc tatttgtgct ctatttttgt cgtagtggcg ttaataccgt ggaatatgcc
     8461 ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata taccccatgc
     8521 gaaattaatc atggactgcg tgatattact ttccgtaacc agttgcctga actcggcgct
     8581 gtataccgcg tcaaggatgc tctactcctt aagccgtcgc ggtgatgcgc ccgcggtaat
     8641 gggcaaaatc aaccgcagta aaaccccgta tgtggcggtg ttactctcca ccggagcggc
     8701 atttttaacg gtcgtggtga actattacgc acctgcgaaa gtgtttaaat tcctgatcga
     8761 cagctccggt gctatcgccc tgctggttta tttagtcatc gccgtttcac agttgcggat
     8821 gcgtaaaatt ctgcgagcag aaggaagcga aattcgcttg cgcatgtggc tttacccgtg
     8881 gctcacctgg ctggtaatcg gctttattac ctttgtgttg gtggtgatgt tattccgccc
     8941 ggcgcaacag ttagaagtga tctctaccgg cttattagcg atagggatta tctgtaccgt
     9001 gccgattatg gcgcgctgga aaaagctggt attgtggcaa aaaacacccg ttcataatac
     9061 gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg ctatcgctgg
     9121 ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg aatgagtttg
     9181 ctgacatcgc gttatgcact tggcgttggg ccgttacggg aagctctttc gcaactggtg
     9241 gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag
     9301 caggagctgc tcgatatttt cgacgcccgc gccaatatgg aagcgatgtt agtgagtctg
     9361 gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc gcatctgctg
     9421 agtaagcttg aggcctgtga cgccagcgag aaaatgcttg atgagtggga tctgcgtcat
     9481 caggcgtttc atactgcaat tgtggcgggc tgtggttctc actatttgct gcaaatgcgt
     9541 gaacggttgt ttgatctggc ggcgcgttat cgatttatct ggctgcggcg aacggtgctt
     9601 tcggtggaaa tgctggagga taaacacgat cagcaccaga ccctgactgc ggcggtactg
     9661 gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac gccaattccc
     9721 attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca acacttgccc
     9781 cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga ttttattgta
     9841 cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc cagacttaac
     9901 ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat caacactggc
     9961 aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct gactcaggcc
    10021 gtcaccagtg accgtcgctt tgccatcggc aatttgaatg ttcactttat cggcatcagg
    10081 tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcatctt tatcgtgctg
    10141 acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga acagacccat
    10201 agctacctca ttcgttaacg gaaacactca gaaagtgtag tagaggaacg cggcgcgtta
    10261 acgccgcggg gatgagcaca ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa
    10321 ccggggatat acccaagcag tgtcaacaga atattaataa tgaacgccca accgaaccct
    10381 ttaccgagca gcacgccgag cggcggcaga atgatggtga tgacgattct ccagaaaccc
    10441 atatgtactc cctataagaa aattacttat tgtttaaaaa gagattttat ctcttaagcg
    10501 tagtaatttt agcggaggct gccagtttca gcttacgctt ttactctcat ttacgctgct
    10561 gaggctggta tatgttgcat ttttagttta gagtttactt aatttagaaa atacttaaat
    10621 aactatgacc gaactcacgc aattacaggc cagtgctgaa caggcagcgg ccttattgaa
    10681 agcaatgagc caccctaaac ggttgctgat tctgtgcatg cttagcggtt cccccggcac
    10741 cagcgcggga gagctgacgc gcattaccgg actgagcgcc tctgcgacat cacagcatct
    10801 cgctcgtatg cgggacgaag ggcttatcaa cagccaacgg gatgcccaac gcattctata
    10861 ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg tctattgtcc
    10921 gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag aattaatcgc
    10981 acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg aacatattcc
    11041 tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtctcccgg ctaaattacg
    11101 tcgcgatcaa attatattcc actgccaggc aggtaaacgc accagtaata acgccgataa
    11161 attagcagcg attgccgccc ccgcagaaat ctttttactc gaagatggga ttgatggctg
    11221 gaaaaaagca ggattaccag tagcggtaaa taaatctcaa cccttgccgt taatgcgcca
    11281 ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt atactgggtt ataccgtaaa
    11341 tagcggtttc ttcttattaa gcggctttgt tggtgccggg ttactgtttg caggaattag
    11401 cggtttttgt gggatggcaa ggttgttaga taagatgccg tggaaccaat gagcttgaga
    11461 agcgacgccg gacgcgccct agcagcgaca tccggcctca gtaattagat caggaaatcg
    11521 tcgagagatt taccttctgc cagcgcctga gcgattggct tcggtgtacg gccctgaccg
    11581 gtccaggttt tagtttcacc gttaacatcg gtgaatttat atttcgccgg acgcggctgg
    11641 cgttttttac cagcacgtgg tgcagcagca gagctattac ccaataactc ttccgggtta
    11701 attccgtcag ctttcatcag ctccagccag gtgctaattt tttcctggcg ctctgccagt
    11761 tcacgctgct gctgttcttc ttcttcacgt ctttctttag tgacaaccct gaatttttcg
    11821 agcatttctt caagaacgtc aatggagaat tcgcgagcca tcgcacggag ggtgcgaatg
    11881 ttatttaaac tttgtaacat tacggacata ataaagaaaa ccttttaacg ccaaaacaaa
    11941 aaagtatttc gtcgcgtagt gtaagcgatc acttattata tttccacaaa atttaatcta
    12001 tttacagtcc tgcgcgagat tatttcagct gttattttct gaaactctca gaataatttc
    12061 aagattatat attagccatt ttgatgagta atttagatta ttggtgaaca gaacggcgct
    12121 ctctgctcgc taattccgca agcctgacaa aaccaaagtg aataaaaatg caatataaca
    12181 gaataaaata aaacacccct cactggtgct ttctgctgtt aatgtacatt ttctggcata
    12241 aaaaatgctg taaatgaccc ggtcacgaca tacaccgaat tctctacgct tatagcgtta
    12301 cctcaccccc aaactttcat tagtgaaatt aaaggaaagg tcatatccaa ttgatttaat
    12361 tgaaaataaa tcatatcggc atgtaaaaat ccacaatgca caaaaaacga gccgttacgg
    12421 aatattttat ctacaaaaac tgactaaata aaaacttttc actaattgat tagtcatagc
    12481 cagcgatata cgctatgcga aaatgcagat ggcaatgaga tccactgctt tcatctccat
    12541 taacatccca ttacgctttt attaaggagc attagcatgt tctcaccgca gtcacgcttg
    12601 cgtcatgcag ttgcagatac gttcgcgatg gttgtttact gttctgtcgt gaacatgtgt
    12661 attgaagttt tcctctccgg aatgagcttc gaacagtctt tttattccag attggtagcg
    12721 attccggtga acatcttaat tgcatggcca tacggtatgt accgcgatct gtttatgcgc
    12781 gcggcacgca aagttagccc gtcgggctgg ataaaaaatc tggcggatat cctggcttat
    12841 gtgacgttcc agtcaccggt gtatgtggcg atcttgttag tggtgggcgc agactggcat
    12901 cagattatgg cggcggtcag ttcaaacatc gttgtttcga tgttgatggg ggcggtttat
    12961 ggctacttcc tcgattattg ccgccgactg tttaaagtca gccgttacca gcaggtaaaa
    13021 gcctgactct tcttttcgcg actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa
    13081 taaaccattc aaataacgtt caagcgcaac acgcgaacta aagccatgcg ggataccgta
    13141 atgctcgtgt cgctcgcctg ctaaatagag cggaaattgc tggtaatgat cgcaagtttt
    13201 aatatcggaa gcgggttcag ccagtgtcga actgcgttct tttaatgttg gatgaattac
    13261 cagagcggta cgtcccattc gcgcttcgcg attaacatag acataatttt ccccacggcg
    13321 gtaaccatac gctttctggg ttaccacatc gacagtaaaa ccgacttttt caagtacgcg
    13381 cgccacctcg tctggtcgta aatacatatt tgatcctcgt tattatcaac cgcgggccta
    13441 ccttacctga ttgcgcattc acaacgcttt cagaaaagtc cataaagccg cgatgaacct
    13501 cagttaagaa aattatggtc tacactgaaa attacatcga attctaatgg gggatcatat
    13561 gtttaaccga ccgaaccgca acgacgttga tgatggcgtg caggatatcc agaatgatgt
    13621 caatcaatta gctgacagcc tcgaatctgt attgaagtcc tggggcagcg acgccaaagg
    13681 ggaagctgaa gccgcacgca gtaaagctca ggcattgctg aaagaaacca gggcgcgaat
    13741 gcatggtcgt actcgcgtcc agcaagccgc gcgcgatgcc gtaggctgcg cggattcttt
    13801 tgttcgtgaa agaccctggt gtagtgtggg aactgctgct gcggtaggta tttttatcgg
    13861 tgcgttgttg agcatgcgta aatcgtagtg agacgatgac aagcagtagt ctgacttctg
    13921 aaaagtactc agtgcgtcaa cgccttgtcc gcctacaggc ctgaaaaaca tagcgcatca
    13981 ggggattttg ccctggtcac cagtcttata ggaccccgaa gcctggcttc ggggttatta
    14041 atttaaagca ctgcgcaatt gctgtgctac ctgtcgcgcc atactttcgc taacgatatt
    14101 cgtaaacgag agcaataatc ctccgtcctc gcttgagcgg atacgccagt cgctaagtgc
    14161 ctgtacggca agaccagcag cattggcttt atgcacgaat gtcacgtcat tacctgctag
    14221 tttgatcacc atctggatac cgcctttttg cggcacaacc tgaaatccct gctgcgtaag
    14281 tgcctgctca atccactgcc gtcgctgggc ataatgctgg cgcattttct tcagatgccg
    14341 ccagaaatgc ccctcacgga tgaaatctgc cagtgtgttc tgccacagta cgggtacagc
    14401 acatggttcc agtgacgcct gctgccggaa ttgcgaaatc tgctccaccg gcaccaccag
    14461 ccacgcacag cgcaatgcag gaaatagcgc tttgctgaat gttccggcat aaatcacccg
    14521 ctgcggcgcg tcgagacttt ttagtgccgg taacggctta ccgtgatagc gaaactcact
    14581 gtcgtaatca tcttcaataa tccatgcctg actacgctct gcccattcca gtatctgata
    14641 cctacgcgct aaagagagtg ccacacccag cgggctttgg tgtgccggag taatcagggc
    14701 aaaacgcgca tcaggataat tttgtattcc gcttgtgaca tccagtccgt tgtcatcaac
    14761 cggcacaggt aaaatttcca caccgtggcg agtgacaatc gggcgaatca gcggaaagcc
    14821 gggatcttcc atccacatcc cgtctcccgg ttgcgccaga gcgtgcagaa ttaaagccat
    14881 tgaggccgca taaccgtgag tgataaagac ctgctctggc tgacaatcaa tcccacgtga
    14941 aacgcgtaaa taatcaacaa tagcctcgcg caaggccgcc tcgccgcaga catcgcctaa
    15001 tgccaaatca aagcgcgtct gggtacgcag acggcggccc atcacccgcg cccacaattc
    15061 acgcggaaaa agatcaagtg caggtagccc catctgaaaa ggcagcggct gctgactttc
    15121 accagcaaaa gccacgacat ccacagcaga ctgttgcgga tgtatctgct cactgacaaa
    15181 ggtacccgcc tgtccccgcc tgaccagcca tccctgcgcc accagctcag cataagcatt
    15241 ttcgacagtg gatcgagaga cacccagctc ctgcgaccag gtacggctcg aaggtagcct
    15301 tgcgccaggt ttcagttttc cttgctcgat aaccgttttt aactgacggg cgatatcctg
    15361 ataacgtggc atatggtctg cttttttagg taatttatgg ctctctttag cagtccatta
    15421 tagtgctaca ttgcgggcgt taataagagg agaaactgta tgactacatt acgtcagcct
    15481 tactacgaac ttagcccggc agtgtataac gcactggtgc aggccaaaac ggcactggaa
    15541 aatagcacgc tggataccac gttgatggag ttaatttatt tgcgcgtctc gcaaatcaac
    15601 ggctgcgcat tttgtctgga gatgcacagc aaagcattgc gcaaatccgg tgtgccacaa
    15661 cacaaactgg acgccctggc aggctggcgc gtaagccatc attttgatga acgcgagcga
    15721 gcggcgctgg catgggcgga atctgtgacc gatattgcca gaacccatgc ggaagacgag
    15781 gtttatcagc ctttgcttga gcatttcagc gcagcagaaa tcagcgactt aacgtttgcc
    15841 atcgggctga tgaattgttt taaccgtctg gccgtttcca tgcggatgta atttgctgtt
    15901 gccgggtatg cgcccggcaa aaaaacacaa aatcccctac cccgttgagc cattcaccgg
    15961 tggcgtaatc aatctaaaat taccatatct tgtgtggttg aatctttttt caactacatc
    16021 tagtatctct gtatcaacag agagacaacc cgacgcgtat catcgcgccg tatcttcatt
    16081 ttaaacggaa atacgaatca tgcgcattac tatttacact cgtaacgatt gcgttcagtg
    16141 ccacgccacc aaacgggcga tggaaaaccg gggctttgat tttgaaatga ttaatgtcga
    16201 tcgcgttcct gaagcggcag aagcgttgcg tgctcagggc tttcgtcagt tgccggtagt
    16261 gattgctggc gatcttagct ggtctggttt ccgtccggac atgattaacc gtctgcatcc
    16321 agcgccacac gcggccagtg catgagccag ctcgtctact tctccagcag ctccgaaaac
    16381 acgcagcgtt ttatcgaacg tttaggtctg cccgcggtgc gcatcccgct caatgagcgg
    16441 gaacggattc aggtagacga gccttacatc ctgatcgtgc cctcttacgg cggcggcggt
    16501 acggctggcg cggtgccacg acaggtaatt cgctttttaa acgacgagca caaccgggcg
    16561 ttgcttcgcg gcgttattgc ttcgggtaat cgcaactttg gtgaggcgta tggccgcgcc
    16621 ggagatgtga ttgcccggaa atgcggcgtg ccgtggctgt accgttttga actcatgggt
    16681 acgcaaagcg atatcgaaaa cgttcgtaaa ggagtaaccg aattttggca acgacaaccg
    16741 cagaatgcct gacgcagaaa acgatggatt accacgcgct gaatgcgatg cttaacctct
    16801 acgatagcgc aggtcgcatt cagttcgata aagaccgcca ggccgttgac gcctttatgg
    16861 cgacgcatgt gcgtccgaac agtgtgacct tcagtagcca gcagcagcgc ctgaactggc
    16921 tggtcaacga aggttactat gatgaaagcg ttcttaatcg ctactctcgc gactttgtca
    16981 ttacgctgtt tgcccacgca cacaccagcg gttttcgttt ccagacattc ctcggggcat
    17041 ggaagtttta caccagctat acgttgaaga cattcgacgg taaacgttat ctggaagatt
    17101 ttgccgatcg agtcacgatg gtggcgctga cgctggcaca aggcgatgag acgctggcgt
    17161 tgcaactgac agatgaaatg ctgtcaggac gctttcagcc agccacgcca acattcctca
    17221 actgcggtaa gcagcagcgc ggcgaactgg tttcctgttt tttgctgcgt attgaagaca
    17281 atatggagtc gattggtcgg gcggtaaatt ccgcactgca actgtcgaaa cgcggcggcg
    17341 gcgtagcatt tttgctgtcg aatctgcggg aagcgggcgc gccaattaaa cgtattgaaa
    17401 atcaatcttc tggcgtaatt ccggtgatga aaatgctgga agacgcattt tcctatgcca
    17461 accaactcgg cgctcgtcag ggggctggtg cagtctattt acatgctcat catcccgata
    17521 ttctgcgttt tctcgacacg aaacgggaaa atgccgacga aaaaatccgc attaaaacac
    17581 tgtcgcttgg cgtggtgatc ccggatatca ctttccatct ggcaaaagag aatgcgcaga
    17641 tggcgctgtt ttcgccttat gacgtagagc gagtttatgg caagccgttt gccgatgtgg
    17701 ccatcagcca acactatgac gaactggttg ccgatgaacg cattcgcaaa aaatacctca
    17761 acgcccgtga tttcttccag cgactggcag aaatccagtt tgagtccggc tatccctaca
    17821 tcatgtatga agacacggta aaccgtgcta accctgtcgc cgggcgcata aatatgagta
    17881 atctctgctc agaaattttg caggttaaca gcgcctcaga gtatgacgag aatctcgact
    17941 atgcccgcac aggccatgat atctcctgca atttaggttc gttgaatatt gcgcacacca
    18001 tggattcccc cgattttgcc cgcacggtag agactgccgt gcgcggttta acggcagtat
    18061 cagatatgag tcatatccgc agcgtgccgt ccatcgaagc cggaaatgcc gcctcgcacg
    18121 ccatcggact ggggcagatg aatttacacg gctatctggc gcgagaaggc atcgcttatg
    18181 gttcgccgga agcactggat ttcaccaatc tctatttcta tgccatcacc tggcacgcac
    18241 tgcgtacctc gatgttgctg gcacgcgaac gcggtgaaac cttcgccggg ttcaaacagt
    18301 cacgctatgc cagtggtgaa tattttagcc aatatctgca agggaactgg cagccgaaaa
    18361 cggggaaagt tggcgaactg tttgcccgta gcggtattac gttacctacc cgtgagatgt
    18421 gggcgcaact gcgcgacgac gtgatgcgct acggcattta caaccagaat cttcaggcgg
    18481 tgccgccaac aggttctatc tcttatatca accatgctac gtcgagtatt catccgattg
    18541 tggcgaaagt agagatacgc aaagagggca aaacaggacg cgtttactac cctgccccgt
    18601 ttatgactaa cgagaatctg gcgctgtatc aggacgctta cgaaattggc gcagaaaaga
    18661 tcatcgacac ctacgcggaa gcgactcgcc atgtcgatca ggggctgtcg ctgacgctgt
    18721 ttttccccga taccgccacc actcgcgata tcaacaaagc gcagatttac gcctggcgca
    18781 aaggtatcaa aacgctctat tacatccggc tgcgtcagat ggcgctggaa ggcactgaaa
    18841 ttgaaggctg cgtctcctgt gcactttaag gaatatctat gaaactctca cgtatcagcg
    18901 ccatcaactg gaacaagata tctgacgata aagatctgga ggtgtggaat cgcctgacca
    18961 gcaatttctg gctaccagaa aaggtgccgc tgtcgaacga tattcctgcc tggcagacat
    19021 taactgtcgt agaacaacaa ctgacgatgc gcgtttttac tggcctgacg ctgctcgaca
    19081 cgctgcaaaa tgttatcggc gcgccttcgc tgatgcccga tgcactcacg cctcatgaag
    19141 aagcggtatt atcgaatatc agctttatgg aagcggttca tgcccgctct tacagttcga
    19201 ttttctcgac gctgtgccag accaaagatg tcgatgccgc ctacgcctgg agtgaagaaa
    19261 acgcgccgtt gcagcgaaaa gcacagatta ttcagcaaca ttaccgcggt gatgatccgc
    19321 tgaaaaagaa aatcgccagt gtgtttcttg aatctttttt gttctattcc ggtttctggc
    19381 tgccgatgta tttttccagc cgcggaaagc tgaccaatac cgcggacctg atccgtctga
    19441 ttatccgcga tgaagcagtc cacggttact acataggcta taaatatcag aaaaacatgg
    19501 aaaagatatc tctgggacaa cgtgaagagt tgaagagttt cgccttcgat ttgttgctgg
    19561 aactctacga caacgagttg caatacaccg atgagctgta cgccgaaacc ccgtgggctg
    19621 acgatgtgaa agcgtttctc tgttacaacg ccaataaggc tttgatgaat ctgggctacg
    19681 aaccgttatt tcccgcagaa atggcggaag tgaatccggc aatcctcgcc gcgctttcgc
    19741 cgaatgccga tgaaaatcac gatttctttt ccggttcagg ctcctcttat gtgatgggga
    19801 aagcggttga aacagaatat gaagactgga atttctgagg gtgttatttt caaaaatatc
    19861 actacccgcg acagggaaat aattcccgcc aaatagcttt ttatcacgca aatattttgt
    19921 ggtgatctac actgatactc tgttgcatta ttcgcctgaa accacaatat tcaggcgttt
    19981 tttcgctatc tttgacaaaa aatatcaact tttctcgatt tgctctcagc ccttatatca
    20041 cgggaaattc cggcgatttg ctcgcatcaa tattcatgcc acatttgcca tcaggggttg
    20101 cctcagattc tcagtatgtt agggtagaaa aaagtgacta tttccattgg gtaatatatc
    20161 gacataaaca aataacagga atctttctat tgcatggcaa ttaaattaga aattaaaaat
    20221 ctttataaaa tatttggtga gcatccacag cgagcgttca aatatatcga acaaggactt
    20281 tcaaaagaac aaattctgga aaaaaccggg ctatcgcttg gcgtaaaaga cgccagtctg
    20341 gccattgaag aaggcgagat atttgtcatc atgggattat ccggctcggg taaatccaca
    20401 atggtacgcc ttctcaatcg cctgattgaa cccacccgcg ggcaagtact gattgatggt
    20461 gtggatattg ccaaaatatc cgacgccgaa ctccgtgagg tgcgcagaaa aaagattgcg
    20521 atggtcttcc agtcctttgc cttaatgccg catatgaccg tgctggacaa tactgcgttt
    20581 ggtatggaat tggccggaat taatgccgaa gaacgccggg aaaaagccct tgatgcactg
    20641 cgtcaggtcg ggctggaaaa ttatgcccac agctacccga atgaactctc tggcgggatg
    20701 cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc cggatatttt attaatggat
    20761 gaagccttct cggcgctcga tccattaatt cgcaccgaga tgcaggatga gctggtaaaa
    20821 ttacaggcga aacatcagcg caccattgtc tttatttccc acgatctcga tgaagccatg
    20881 cgtattggcg accgaattgc cattatgcaa aatggtgaag tggtacaggt cggcacaccg
    20941 gatgaaatta tcaataatcc ggcgaatgat tatgtccgta ccttcttccg tggcgttgat
    21001 attagtcagg tattcagtgc gaaagatatt gcccgccgga caccgaatgg cttaattcgt
    21061 aaaacccctg gcttcggccc acgttcggca ctgaaattat tgcaggatga agatcgtgaa
    21121 tatggctacg ttatcgaacg cggtaataag tttgtcggcg cagtctccat cgattcgctt
    21181 aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc tgattgatgc gccgttagca
    21241 gtcgatgcac aaacgcctct tagcgagttg ctctctcatg tcggacaggc tccctgtgcg
    21301 gtgcccgtgg tcgacgagga ccaacagtat gtcggcatca tttcgaaagg aatgctgctg
    21361 cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa aataatccgt gggataccac
    21421 gccagcggcg gacagtgccg cgcaatccgc agacgcctgg ggtacaccgg cgactgcacc
    21481 gactgacggc ggtggtgctg actggctgac cagtacgcct gcgccaaacg tcgagcattt
    21541 taatattctc gatccgttcc ataaaacgct gatcccgctc gacagttggg tcactgaagg
    21601 gatcgactgg gtcgttaccc atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga
    21661 ttatatcctc aacggtttcc agcaattgct gctgggtatg cccgcaccgg tggcgattat
    21721 cgttttcgct ctcatcgcct ggcagatttc cggggtcgga atgggcgtgg cgacgctggt
    21781 ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag gcaatggtta ctctggcgct
    21841 ggtgttaacc gccctgctgt tctgtatcgt catcggtttg ccgttgggga tctggctggc
    21901 gagaagtccg cgagcggcga aaattattcg tccactgctt gatgccatgc agaccacgcc
    21961 agcgtttgtt tatctggtgc caatcgtcat gctatttggt atcggtaacg tgccgggcgt
    22021 ggtggtgacg atcatctttg ctctgccgcc gattatccgt ctgaccattc tggggattaa
    22081 ccaggttccg gcggatctga ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat
    22141 gctgttcaaa gttcagttac cgctggcgat gccgaccatt atggcgggcg ttaaccagac
    22201 gctgatgctg gccctttcta tggtggtcat cgcctcgatg attgccgtcg gcgggttggg
    22261 tcagatggta cttcgtggta tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt
    22321 cgggattgtg atcctcgcca ttatcctcga tcgtctgacg caggccgttg ggcgcgactc
    22381 acgcagtcgc ggcaaccgtc gctggtacac cactggccct gtcggtctgc tgacccgccc
    22441 attcattaag taactctgca cttgcccggt gacgccgggc atatcaccct gccaaaaaaa
    22501 ggaataacaa tgcgacatag cgtacttttt gcgacagcgt ttgccacgct tatctctaca
    22561 caaacttttg ctgccgacct gccgggcaaa ggcattactg ttaatccggt tcagagcacc
    22621 atcaccgaag aaactttcca gacgctgctg gtcagccgcg cgctggagaa attaggttat
    22681 accgtcaata aacccagcga agtggattac aacgttggct acacctcgct tgcttccggc
    22741 gatgcaacct tcaccgccgt gaactggacg ccactgcatg acaacatgta cgaagctgcc
    22801 ggtggcgata agaaatttta tcgtgaaggg gtatttgtta acggcgcggc acagggttac
    22861 ctgatcgata agaaaaccgc cgaccagtac aaaatcacca acatcgcaca actgaaagat
    22921 ccgaagatcg ccaaactgtt cgataccaac ggcgacggaa aagcggattt aaccggttgt
    22981 aaccctggct ggggctgcga aggtgcgatc aaccaccagc ttgccgcgta tgaactgacc
    23041 aataccgtga cgcataatca ggggaactac gcggcgatga tggccgacac catcagtcgt
    23101 tacaaagagg gcaaaccggt gttttactac acctggacgc cgtactgggt gagtaatgag
    23161 ctgaagccag gcaaagatgt ggtctggttg caggtgccgt tctctgcact gccgggcgat
    23221 aaaaatgccg ataccaaact gccgaatggc gcgaattacg gcttcccggt cagcaccatg
    23281 catatcgttg ccaataaagc ctgggccgag aaaaacccgg cagcagcgaa actgtttgcc
    23341 attatgcagt tgccagtggc agatattaac gcccagaacg ccattatgca tgacggcaaa
    23401 gcctcagaag gcgatattca gggccacgtt gatggctgga tcaaagccca ccagcaacag
    23461 ttcgatggct gggtgaatga ggcgttggca gcgcagaagt aatttttatt cgggcggata
    23521 aggcgtttac gccgcatccg ccgttctgta cacaatgcct gatgcgacgc tggcgcgtct
    23581 tatcatgcct acaaaccaca ccgcacattt caacaatcta tctttcatcc catattcatc
    23641 aacatccgct attattgatt tccagcttaa tcatcacctg atgaacaaaa ataatgacca
    23701 aacctaatca tgagcttagc ccggcgctaa tcgtgctgat gtctatcgcc accggtctgg
    23761 cggtcgccag caactattac gcccagccat tgctcgacac catcgcgcgt aacttttccc
    23821 tttccgccag ttcggcaggc tttattgtta ccgccgcgca gttgggctat gccgccggtc
    23881 tgctgtttct tgttcccctc ggtgatatgt ttgaacgccg ccgcctgatt gtctcgatga
    23941 ccttactggc agcgggcggt atgttaatca ctgccagcag tcagtcactg gcgatgatga
    24001 tcctcggtac ggcattaacc ggtttattct cagtcgtggc acaaattctg gttccgctgg
    24061 cagcgacgct ggcttcaccg gataaacgcg gcaaagtggt tggcaccatt atgagcggtc
    24121 tgctgttggg gatattgctg gcacgaaccg ttgccgggtt actggcaaac ctcggcggct
    24181 ggcgcaccgt cttttgggtt gcctcggtgt taatggctct gatggcgctg gcattatggc
    24241 gtggtctgcc acaaatgaaa tcagaaaccc acctcaacta cccacagttg ttgggttccg
    24301 ttttcagcat gtttatcagc aataagatcc tgcgcacccg cgcgttgctg ggctgcctga
    24361 cctttgccaa cttcagcatt ctctggacct caatggcctt tttgcttgcc gctccacctt
    24421 ttaactacag cgatggtgta attggtctgt ttgggcttgc gggagctgcc ggagcgttgg
    24481 gcgctcgtcc ggcgggcggt tttgccgaca agggcaaatc gcaccacacc acaactttcg
    24541 gtctgcttct gctgttactt tcatggctgg caatctggtt tggtcacact tccgtactgg
    24601 cgttgattat cggcattctg gtgctggacc tcaccgtaca gggcgtgcat atcactaacc
    24661 agacggtaat ttatcgaata catcctgatg cgcgtaatcg cctgaccgca ggttacatga
    24721 ccagctactt tattggcggt gccgccggtt cgctaatttc agcctcagcc tggcaacatg
    24781 gcggttgggc tggcgtttgt ctggctggcg cgacgattgc cctggttaac ttactggtct
    24841 ggtggcgagg ttttcatcgt caggaagccg caaattaagc aatcagcaac cgttttcgtg
    24901 gctttacact tataagggtg ttaagaagcc catcagtctg ataaggttaa gatattcatt
    24961 cagtctattt ataatattaa caatcgttaa gcgtacactc tatggaaagc cctactccac
    25021 agcctgctcc tggttcggcg accttcatgg aaggatgcaa agacagttta ccgattgtta
    25081 ttagttatat cccggtggcc tttgcgttcg gtctgaatgc gacccgtctg ggattctctc
    25141 ctctcgaaag cgtttttttc tcctgcatca tttatgcagg cgcgagccag ttcgtcatta
    25201 ccgcgatgct ggcagcaggg agtagtttgt gggttgctgc actgaccgtc atggcaatgg
    25261 atgttcgcca tgttttgtat ggcccgtcac tgcgtagccg tattattcag cgtctgcaaa
    25321 aatcgaaaac cgccctatgg gcgtttggcc tgacggatga ggtttttgcc gccgccaccg
    25381 caaaactggt acgcaataat cgccgctgga gcgagaactg gatgatcggc attgccttca
    25441 gttcatggtc atcgtgggta tttggtacgg taataggggc attctccggc agcggcttgc
    25501 tgcaaggtta tcccgccgtt gaagctgcat taggttttat gcttccggca ctctttatga
    25561 gtttcctgct cgcctctttt cagcgcaaac aatctctttg cgttaccgca gcgttagttg
    25621 gtgcccttgc aggcgtaacg ctattttcta ttcccgtcgc cattctggca ggcattgtct
    25681 gtggctgcct cactgcgtta atccaggcat tctggcaagg agcgcccgat gagctatgag
    25741 gttctgctgc ttgggttact ggttggcgcg gcgaattatt gcttccgcta tttgccgctg
    25801 cgcctgcgtg tgggtaatgc ccgcccaacc aaacgtggcg cggtaggtat tttgctcgac
    25861 accattggca tcgcctcgat atgcgctctg ctggttgtct ctaccgcacc agaagtgatg
    25921 cacgatacac gccgtttcgt gcccacgctg gtcggcttcg cggtactggg tgccagtttc
    25981 tataaaacac gcagcattat catcccaaca ctgcttagtg cgctggccta tgggctcgcc
    26041 tggaaagtga tggcgattat ataacccaca agaatcattt ttctaaaaca atacatttac
    26101 tttatttgtc actgtcgtta ctatatcggc tgaaattaat gaggtcatac ccaaatggat
    26161 agttcgttta cgcccattga acaaatgcta aaatttcgcg ccagccgcca cgaagatttt
    26221 ccttatcagg agatccttct gactcgtctt tgcatgcata tgcaaagcaa gctgctggag
    26281 aaccgcaata aaatgctgaa ggctcagggg attaacgaga cgttgtttat ggcgttgatt
    26341 acgctggagt ctcaggaaaa ccacagtatt cagccttctg aattaagttg tgctcttgga
    26401 tcatcccgta ccaacgcgac gcgtattgcc gatgaactgg aaaaacgcgg ttggatcgaa
    26461 cgtcgtgaaa gcgataacga tcgccgctgc ctgcatctgc aattaacgga aaaaggtcac
    26521 gagtttttgc gcgaggtttt accaccgcag cataactgcc tgcatcaact ctggtccgcg
    26581 ctcagcacaa cagaaaaaga ccatctcgag caaatcaccc gcaaattgct ctcccgtctc
    26641 gaccagatgg aacaagacgg tgtggttctc gaagcgatga gctaacgcgt catctcgctc
    26701 aaaaatccag atttataaaa gaaaaaatga ccggccagca tcgcaacatg ctggcctttt
    26761 tggcaagcag gtcggctcag ccgatgagtt aagaagatcg tggagaacaa tatgagcgca
    26821 aatgcggaga ctcaaacccc gcagcaaccg gtaaagaaga gcggcaaacg taagcgtctg
    26881 ctcctccttc tcaccttgct ctttataatt attgccgtag cgatagggat ttattggttt
    26941 ttggtactgc gtcacttcga agaaaccgat gacgcatacg tggcagggaa tcaaattcaa
    27001 attatgtctc aggtgtctgg cagcgtgacg aaagtctggg ccgataacac cgattttgta
    27061 aaagaaggcg acgtgctggt cactctcgac ccgacagatg ctcgccaggc gtttgaaaaa
    27121 gccaaaactg cactggcttc cagcgttcgc caaacccacc agcagatgat taacagcaag
    27181 cagttgcagg cgaatattga ggtgcaaaaa atcgccctcg cgaaagcaca aagcgactac
    27241 aaccgccgtg tgccgctggg caatgccaac ctgattggtc gcgaagagct gcaacacgcc
    27301 cgcgacgccg ttaccagtgc ccaggcgcaa ctggacgtcg cgattcaaca atacaatgcc
    27361 aatcaggcga tgattctggg gactaaactg gaagatcagc ctgccgtgca acaggctgcc
    27421 accgaagtac gtaacgcctg gctggcgctg gagcgtactc gtattgtcag tccgatgacc
    27481 ggttatgtct cccgccgcgc ggtacagcct ggggcgcaaa ttagcccaac gacgccgctg
    27541 atggcggtcg ttccagccac caatatgtgg gtggatgcca actttaaaga gacgcagatt
    27601 gccaatatgc gtatcggtca gccggtcact atcaccaccg atatttacgg cgatgatgtg
    27661 aaatacaccg gtaaagtggt tggtctggat atgggcacag gtagcgcgtt ctcactgctt
    27721 ccggcgcaaa atgcgaccgg taactggatc aaagtcgttc agcgtctgcc tgtgcgtatc
    27781 gaactggacc agaaacagct ggagcaatac ccgctgcgta tcggtttatc cacgctggtg
    27841 agcgtcaata ccactaaccg tgacggtcag gtactggcaa ataaagtacg ttccactccg
    27901 gtagcggtaa gcaccgcgcg tgaaatcagc ctggcacctg tcaataaact gatcgacgat
    27961 atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg atgcaacagc aaaaaccgct
    28021 ggaaggcgcg caactggtca ttatgacgat tgcgctgtca ctagcgacat tcatgcaggt
    28081 gctggactcc accattgcta acgtggcgat ccccactatc gccgggaatc ttggctcatc
    28141 gctcagccag ggaacgtggg taatcacttc tttcggggtg gcgaatgcca tctcgatccc
    28201 gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa ctgttcctct ggtcaaccat
    28261 cgcctttgct attgcgtcgt gggcgtgtgg tgtctccagc agcctgaata tgctgatctt
    28321 cttccgcgtg attcagggga ttgtcgccgg gccgttgatc ccgctttcgc aaagtctatt
    28381 gctgaataat tacccgccag ccaaacgctc aatcgcgctg gcgttgtggt cgatgacggt
    28441 gattgtcgcg ccaatttgcg gcccaatcct cggcggatat atcagcgata attaccactg
    28501 gggctggata ttcttcatca acgtgccgat tggcgtggcg gtggtgttga tgacactgca
    28561 aactctgcgc ggacgtgaaa cccgcaccga acggcggcgg attgatgccg tggggctggc
    28621 actgctggtt attggtatcg gcagcctgca gattatgctc gaccgcggta aagagctgga
    28681 ctggttttca tcacaggaaa ttatcatcct taccgtggtg gcggtggtgg ctatctgctt
    28741 cctgatcgtc tgggagctga ccgacgataa cccgatagtc gatctgtcgt tgtttaagtc
    28801 gcgcaacttc accatcggct gtttgtgtat cagcctcgcg tatatgctct acttcggcgc
    28861 gattgttctg ctgccgcagt tgttgcagga ggtctacggt tacacggcga cctgggcagg
    28921 tttggcctct gcgccggtag ggattattcc agtgatcctg tcgccgatta tcggccgctt
    28981 cgcgcataaa ctggatatgc gccgactggt aaccttcagc tttattatgt atgccgtctg
    29041 cttctactgg cgtgcctata cctttgaacc aggtatggat tttggcgcgt cggcctggcc
    29101 gcagtttatc caggggtttg cggtggcctg cttctttatg ccgctgacca ccattacgct
    29161 gtctggtttg ccaccggaac gactggcggc ggcatcgagc ctctccaact ttacgcgtac
    29221 gctggcgggg tctatcggca cgtcgataac cacaaccatg tggaccaacc gcgagtcgat
    29281 gcaccatgcg cagttgactg agtcggtaaa cccgttcaac ccgaatgccc aggcgatgta
    29341 cagtcaactg gaagggcttg ggatgacaca acagcaggca tcaggctgga ttgcccagca
    29401 gatcaccaat caggggctga ttatttccgc caatgagatc ttctggatgt cggccgggat
    29461 attcctcgtc ttgctggggc tggtgtggtt tgctaaaccg ccctttggcg caggtggcgg
    29521 cggaggcggt gcgcactaag tacaactaag ccagttcatt tgaactggct tttttcaatt
    29581 aattgtgaag atagtttact gactaaatgt gcagttcctg caacttctct ttcggcagcg
    29641 ccagttcttc gttgctgttg atgcgcacgt cacgttccag aatgctacgc gcaatatcct
    29701 gcgcttcctg caacgagtgc atctggtaag tgccacactg gtagacgttc agctccggaa
    29761 tctgattctg atcctgcact ttcagcacgt cttccattgc cgctttccag acatcagcaa
    29821 cacgctgctc atctggcgta ccaatcagac tcatataaaa accggtgcgg cagcccattg
    29881 gcgagatatc gataatctct acaccattac cgttaagatg gttacgcata aaaccagcaa
    29941 acaggtgctc cagggtatgg atccctcttt ctggcatcac ttctttgttc ggcacgcaga
    30001 agcgcagatc gaacacggtg attgcgtcgc catgcggggt gttcattgtt ttcgccaccc
    30061 gaactgcagg cgcttccatc cgggtatgat cgactgtgaa gctatctaac aacggcattt
    30121 agccacctcc ggtaattttt ttaaaaattt tctgaactct ttcttcccag gcgagtctga
    30181 gtatatgaaa gacgcgcatt tgttatcatc atccctgaat tcagagatga aattttggcc
    30241 actcatgagt ggcctttttc ttttctgtca ggcgtgtttt tccagccaca ccgcaaacgg
    30301 ttcggtatct gcggcttcca tttcctgctg acggcgttca gatgcctcgc gctcggctac
    30361 aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc agcagattac ggtacgcttc
    30421 agcaaacgct ttgcctgttc cgccaatacc agtatcaatc atagacctta agatacgggc
    30481 agagaaagtc agatcgggat tatcgaagca ggcaaccagt tcatcacaca ctttctgata
    30541 cgcttcgccg ccgttaatgc tatccagcgt ttgcgcgacg cgtttcagat cgcggaacag
    30601 atctttaccc acctgcggta acgggaactg tgcggtttcg cagccgatac ccagcgtcag
    30661 acccggtttg cgaccttcga ggatcacccg gttccagtta acgcgtgtac aggcaagttc
    30721 gctactgctc atttccggtg catcagccag cgcacaccag accataaaca ggtcgaggaa
    30781 tcgcacctgc tgttcatcta caccaatcgg cgagaacggg ttgatgtcca gcgaacgcac
    30841 ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa ggcgactcgc cgctgcgggt
    30901 aacgcgtttt ggacgaatcg gcgcgtacag ttcgttttca atctgcagca cgttgctgtt
    30961 gatttgcagc ctcttaccgt ctttatcaat accaatcttc gcgtactctt ccgatggcgt
    31021 tttgattgcc tgtttcaggc ccgctacgta ctcgtaaaga tcgttgaagg taataccaag
    31081 attgctttgc gatttattgg tatagccgag atcgctcaaa cgaagagagg tcgcatacgg
    31141 caggtaatac ataccgcact cggttttctc aaacggcagc gacgttggtt taccttgcag
    31201 gaatgaagag catattgccg gagacgcgcc aaacagataa ggaatgaccc aaccgaagcg
    31261 atagtagttg cgaataacgc ggaaatagcc cgcagagatt ttctctttag catcagcgcc
    31321 cgaaatatcg ccacacttcg cttgccagaa tgccataggc aaagagaagt tgtaatgcac
    31381 gccggagatg gtttgcatca gcgcgccgta gcgatttttc agcccttcac ggtacagcgt
    31441 tttaaagcgt ccggtgttag aagtgccgta ctgcgccagt tcgatgtcct gaccttctgc
    31501 gatgtagcat ggcatactta acggccacat ccgctcatcg cccatattgc gcgccgtata
    31561 acgatgcaga tcgcgcataa aggtcagcat atgttcaata tcaccatcca ctggtgtaat
    31621 gaattccagc aatgcttccg caaaatcggt agtaatccat ttatgcgtca gtgcggaacc
    31681 taatgcttca ggatgacctg ttgttgccag tgtgccatca gcattaacac gcaaagtttc
    31741 gcgctccagc ccacgctgta tcccctttaa cgcctgagga tgtttttcca gccaggccag
    31801 cgcctgtgat acgtccggga tcaaattgac ctcccgcctg tcaaaatcgt tttaattagc
    31861 ataactgtaa tggtgaccat atgtgcaggc ctacaattag tgccaccaca tcatgccctg
    31921 aacggtcgct gctgcaactg caacatagcg taacgcttta ccaaggcaca aaaaaaagat
    31981 taccggcccc caggagatgc gcatccatcc cgctaacaga cacagtaaat cgccaaccac
    32041 gggcatccag cttaataata gcgtgactgc accatagcgt ttcagccagc cggtagcttt
    32101 ctcttgccag cgcgatgttt tacgcaatgg acagaaacgc ccaaggataa cgttagttaa
    32161 ccctccaagg ctattaccca ttgttgctgt taaaactaaa acccagggat gactgatccc
    32221 agaaagcaac attgccacca gcacgacttc ggagttgccg ggtaatagtg tagcgctgag
    32281 aaaactactg gcaaacaacg agaaaagcga taacgcttca ctcacagcaa gcgaacatcc
    32341 acagcgtcca tgcctgctgc acgggccgcc tgaataccga aatcggcatc ttcaaagacc
    32401 acacactgcg tcggttgcac gcccatacgc tgcgcgcaca acaaaaatgt gtctggcgcg
    32461 ggtttatggt gtttgacgtg atcggcagcg acgacggcgt caaaataacg gcgtaatccc
    32521 aggtgagcca gcaatgcctc agcgatggcg ctttcactcc ccgttcctac agccattggg
    32581 cgacgaccat gccaactttt caccacttca acaagaggaa gcggctcgac actatccagc
    32641 agcatacttc ttactgcttc tgttttttca cgcgctaacg catgcgggtc gagatcggcc
    32701 tgattcagct caataattgc ctgagcaata cgccaggtgg gcgatccatt aagcgcaatc
    32761 atcgcctgaa catcgtactg aagaccgtag tgccctaata cttcgcgcca cgctttacgg
    32821 tgcgtaggct ccgtatccag gattgtgcca tccatatcaa aaattaaacc tgcataacgc
    32881 tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta ctttagcgta attgcttgat
    32941 tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg gataaagtag ttatttagaa
    33001 atgagatatt tttgaaggaa attttttgga gaagatggtg catccgggag gatgactcac
    33061 tggcgttcgc ccttcgggcc gttgctaaag caacgttatc cttcctggtg cctgcggtta
    33121 attccgctac ctcttcgata tcttcattgc tgaagatttc ggagaatatg gtgcatccgg
    33181 gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg
    33241 gatgcatcgg gaaaatttat tttactgctg atattgatat cgctactaaa gccatatcaa
    33301 ataagaaaat ccatcttaac catcatcact gatgattacg gagaatatgg tgcatccggg
    33361 aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg
    33421 atgcatcggg aaatttattt tactgctgat attgataccg ctactaaagc tgtatcaagt
    33481 aagagatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta gccgagtact
    33541 ctatccagct gagctacgga tgcatcggga aacttacttt actgcagatt ttttgatacc
    33601 gctactaaag ccgtatcaag taagagatgg tgcatccggg aggattcgaa cctccgaccg
    33661 ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg
    33721 gggattcgaa ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc
    33781 agcctctcgg ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc
    33841 tcatcgtcgc tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc
    33901 ccacactttt atcgtttgca cacttcacgc tcaattagtc tgtaaaaacg tcaaaaagag
    33961 tgttttatct acagaaaaac ggaggtctgg cagatagcag taatgcaaaa aaatggagac
    34021 ttaagttgaa tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg
    34081 gtgcgtctca ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt
    34141 tcagcctgga tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt
    34201 acgccaatac gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca
    34261 atcatgaggg tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat
    34321 taaaagagtc gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag
    34381 caatgacaaa cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt
    34441 agccgatata cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc
    34501 gtgaagcgag tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag
    34561 cctttcacac tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc
    34621 atgtcaggac gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc
    34681 cctgctttca cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct
    34741 tcggctaccg ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc
    34801 atggtacgca acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca
    34861 ttaacatcaa ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta
    34921 agctgttgta gttctttttc taactgacgc gtacgttcca gtactgagcg cactttatcc
    34981 gccagattat tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct
    35041 gcatgaacgg tggtgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca
    35101 gcagtacccg attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga
    35161 gtaccgccac acagctcggt ggagaaatcg cccatgctca gcacgcgtac acgctcatca
    35221 tacttctcgc cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg
    35281 ttggtttcga tcggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga
    35341 atctcttccg gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac cttgtcgtta
    35401 accagtgaac ctttctgcga tacatgagtc cccagaacct ggcgcagcgc agcgtgcatc
    35461 aggtgcgttg cggagtgatt caaacgaata cgggcgcgac gagcctcatc aacatcagcc
    35521 tgcacagcgt cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc
    35581 tggccgtatt tctgcgtatc ttccaccacg aaggagaagt tagcgccttt cagttcgcct
    35641 ttatcaccaa cctgaccgcc ggattccgca tagaatggcg tttgatccag cacgaccaca
    35701 gcttcctggc ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact
    35761 ttgccgttca gttccagatg gtcatagcct ttaaattcag atgcactgtc aacacggatc
    35821 attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg cccgacgacg ctgctcttcc
    35881 attgctgctt caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc
    35941 gtcaggtcaa ccgggaagcc ataggtgtcg tacagacgga aagcagtttc accatccagc
    36001 gtatcaccag aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta
    36061 cgagcaaact gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc
    36121 aggtcttcac ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag
    36181 gtttctttcg cgccgagcat attaccgtga cgcactgcgc gacgaatgat acgacgcagt
    36241 acataaccac ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa
    36301 cgaatgtggt cagcgattac gcgcagcgat ttattgctca gatcggttgc gccagtgact
    36361 ttcgctaccg cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt
    36421 tgcagcaccg cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcggcagc
    36481 ggttccatcg tgccatcggc ctggcggttg aactgcatga agacgatgtt ccagatctca
    36541 atgtagcggt cgccgtcttc ttccggactt cccggagggc caccccaaat gtggtcgccg
    36601 tgatcgtaga agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag
    36661 ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg
    36721 atccctactt ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc
    36781 cacagacgct ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac
    36841 tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag
    36901 gtatggtgac gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc
    36961 acgcagcgtt gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca
    37021 tccttgaact ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc
    37081 agggagctgc tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga
    37141 cggatctcag cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc
    37201 accagctcca gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt
    37261 tttcttgact gggaagtaaa ataccgtatg cgttcagtcg gcaaaatttc gccaaatatc
    37321 ctggatatct tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc
    37381 tgaaaagaca gttggcaaag gttcaccata ttttcgcgtc gcctgatcgc gcgccagtgc
    37441 gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt
    37501 ctgattcagt tcctggcgaa tacgcgcagg tccataacct ttgcggctac ggctggcgat
    37561 aaagcgcgca acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac
    37621 gcgctcgtaa tcttctgccg tagcatcaat ctcttctggg ccatttttgc ccataatcgg
    37681 tgccgcgagt ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac
    37741 cgcacgatcc aacaggcgag catatgccgg gcgacgggac gttgattctg tcatggcata
    37801 tccttacaac ttaaaaagca aaagggccgc agatgcgacc cttgtgtatc aaacaagacg
    37861 attaaaaatc ttcgttagtt tctgctacgc cttcgctatc atctacagag aaatccggcg
    37921 ttgagttcgg gttgctcagc agcaactcac gtactttctt ctcgatctct ttcgcggttt
    37981 ccgggttatc tttcagccag gcagttgcat tcgctttacc ctgaccgatc ttctcacctt
    38041 tgtagctgta ccacgcgcct gctttctcga tcagcttctc ttttacgccc aggtcaacca
    38101 gttcgccgta gaagttgata ccttcgccgt agaggatctg gaattcagcc tgtttaaacg
    38161 gcgcagcgat tttgttcttc accactttca cgcgggtttc gctacccacc acgttttcgc
    38221 cctctttcac cgcgccgata cgacggatgt cgagacgaac agaggcgtag aatttcagcg
    38281 cgttaccacc ggtagtggtt tccgggttac cgaacatcac accaattttc atacggatct
    38341 ggttgatgaa gatcagcagc gtgttggact gcttcaggtt acccgccagc ttacgcatcg
    38401 cctggctcat catacgtgcc gcaaggccca tgtgagagtc gccgatttcg ccttcgattt
    38461 ccgctttcgg cgtcagtgcc gccacggagt caacgacgat aacgtctact gcgccagaac
    38521 gcgccagggc gtcacagatt tccagtgcct gctcgccggt gtccggctgg gagcacagca
    38581 ggttgtcgat atcgacgccc agtttacgtg cgtagattgg gtccagcgcg tgttcagcat
    38641 cgataaacgc acaggtttta ccttcacgct gcgctgcggc aatcacctgc aacgtcagcg
    38701 tggttttacc ggaagattcc ggtccgtaga tttcgacgat acggcccatc ggcagaccac
    38761 ctgccccaag cgcgatatcc agtgaaagcg aaccggtaga gatggtttcc acatccatgg
    38821 aacggtcttc acccaggcgc atgatggagc ctttaccaaa ttgtttctca atctggccca
    38881 gtgctgccgc caacgctttc tgtttgtttt cgtcgatagc catttttact cctgtcatgc
    38941 cgggtaatac cggatagtca atatgttctg ttgaagcaat tatactgtat gctcatacag
    39001 tatcaagtgt tttgtagaaa ttgttgccac aaggtctgca atgcatacgc agtagcctga
    39061 cgacgcaccg catcacggtc gccgctgaag cattcccgca gggtaatgcc ttcaccgcgg
    39121 gcagtggcaa aagcaaacca gacggtgccg acaggcttct cttcactgcc gccatccggc
    39181 ccggcgatac cactaataga cacggcataa tcagcacgag ccgctttcag tgcacctatc
    39241 gccatttcca ccacgacggg ttcactcacc gcgccatgct gcgccagcgt ctcttcgcgt
    39301 acgccgatca tttgcgcttt ggcttcgtta ctgtaggtga caaatccgcg ttcaaaccag
    39361 gcggaactgc cggcaatatc ggtaatcact ttcgctaccc aaccgccggt acaagactca
    39421 gcagttgtta cggttgcgcc acgggctttc agcgcctgcc caacctgttc gcttaactgc
    39481 atcagttcac tgtcagtcat cacaacctct gtcagtcaaa aatttatgcc ggacaagata
    39541 gcactttcgt caggaagatg gtgttggcgt tgcaatttta cgaggaggat tcagaaaaaa
    39601 gctgattagc cagagggaag ctcacgcccc cctcttgcaa atagttactg cacgcgcgcc
    39661 agcgccacgg cttgtcctaa ctgccagacc gccattgcgt aatgggtact gtggttataa
    39721 cgggtaatgg tgtagaagtt cggcagaccg taccagtact ggtaacctgt tccaacatcc
    39781 agacgcagca ggctggcttg ttgatggttg cccagcggct gctgtggcgt taaacctgcc
    39841 gcggcaagct gcgaaatgct gtacctggtt ttgaagccat ttggcaagcc tggagcctga
    39901 ccgtttgcca ttaccgcgac ctgatcgcct ttcacccagc cgtgcgcttt gaaataattc
    39961 gccacgctac cgatcgcatc aaccggatcc cacaggttga tatgcccgtc gccgctgaaa
    40021 tctaccgcat attgtttgta agacgacggc ataaactgtc cgtagcccat cgccccggca
    40081 aaggaacctt tcagattgag cggatcgtcc tgctcgtcgc gcgccatcag caggaaggtt
    40141 tccagctcgc cagagaaata ctccgcgcgg cgtgggtagt taaatgacag cgttgccagc
    40201 gcatcgagga tgcgagtttt ccccatcacg cgcccccagc gggtttcaac gccgataatc
    40261 ccgacgataa tttccggcgg tacgccatac acctgccacg cgcgattcaa cgcatcttca
    40321 tactgattcc agaaaaccac accgttctgc acattgtccg gcgtaataaa ttttttgcga
    40381 taacgcagcc atgcgccgtt cggacctgat ggcggtttca ccgatgtggt tggtgcctgg
    40441 ttatccatca gccgcagtac cgaatccaga cgtttcgcct gggagagaat ttcctgcaac
    40501 tgctgacgat cgaaaccgtg tttgttcacc attctgtcga tgaactgctg ggcattcggg
    40561 ttattagcga aatcgccgcc catctgcatc acattgtgct gcggctcaag caggaagccg
    40621 ccagacggcg ttccggtggt cgtttcagtc tcagtaggtt ttggcttgct gctacaggcg
    40681 gcaagcaaca caaaaagggg aagcaatgtt acataacgac gcttgaacat gaggggtcca
    40741 tttaacagat tcaaccaggg gcaagtatgg taaagcatca cgccccgcac aaggaagcgg
    40801 tagtcactgc ccgatacgga ctttacataa ctcaactcat tctcctcgct atccttttac
    40861 tcaaactttc aaattaaaat atttatcttt cattttgcga tcaaaataac actttaaatc
    40921 tttcaatctg attagattag gttgccgttt ggtaataaaa caataaatcc tgaaggagag
    40981 aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt tccaaaaggg
    41041 cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta gcctgctggt
    41101 tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt ttgctcaacg
    41161 ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca cgtttgtctt
    41221 ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca aacccagcta
    41281 ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc atatcaaccc
    41341 tggcgaactg tttgttatct tggcattgcc agcggtctga caacgctgaa cctgccactt
    41401 ggcccactgg cggtgagcta tctgctggtt ggtctggtca ccaacttctt ccgcggctgg
    41461 gtgaccgatc tgaccaccgc tatttttgag aaaaagatgg gcattcaact tgaacaaaaa
    41521 gttcatctgg caggagcaac atcatgacgc atattcggat cgaaaaagga acggggggct
    41581 ggggcggccc gcttgagctg gaaaccacgc cgggcaaaaa aatcgtctat atcaccccgc
    41641 cggtacccgg cctgcgattg tcgacaaact ggcacagctt actggctggc aggctattga
    41701 cggatttaaa gaaggtgaac ccgcggaggc ggaaattggt gtcgcggtaa tcgactgtgg
    41761 cggcacatta cgctgcggca tttatccgaa acggcgtatt cccaccatta atatccactc
    41821 gacgggcaag tccggtccac tggcgcagta cattgtggaa gatatttatg tctctggcgt
    41881 aaaagaagaa aacatcactg tagtgggtga tgcgacacca caaccctctt ccgtgggccg
    41941 tgactatgac accagcaaga aaatcaccga acaaagcgat ggtttactgg cgaaggtggg
    42001 aatgggtatg ggttctgccg ttgccgtgtt gtttcaatct ggtcgtgaca ccatcgacac
    42061 tgtattaaaa accattcttc cgtttatggc attcgtctcg gcgctcattg gcatcattat
    42121 ggcttctggc cttggtgact ggattgccca cggccttgct ccgctggcga gccatccact
    42181 gggtctggtc atgctggcgc tcatctgctc cttcccgctg ctttcacctt tcctcggccc
    42241 aggcgcagtt atcgcacagg ttatcggcgt attgattggc gtgcagattg gtctcggcaa
    42301 tattccgccg catctggctt tacctgcact gtttgccatc aacgcgcagg cggcctgcga
    42361 cttcattccg gtcggtttgt cgctggcgga agcccgtcag gacacggttc gcgtcggtgt
    42421 cccttctgta ctggtgagcc gctttttaac cggcgcgccg actgtactga tcgcctggtt
    42481 tgtctccggt tttatctatc aatagaggct gaaacatgac cgttatttat cagaccacca
    42541 tcacccgtat cggcgcgagt gccactgacg ccctcagcga ccagatgctc atcacctttc
    42601 gtgaaggcgc gccagcggac ctcgaagagt attgcttcat tcattgccac ggcgagttga
    42661 aaggtgcact ccatcccggt ttgcaattta cgctcgggca gcatcgctat ccggtgaccg
    42721 ctgttggcag cgtggcggaa gacaaccttc gcgaactggg tcatgtcacc ctgcgcttcg
    42781 atggtttaaa cgaagcggaa tttccgggca ctgtccatgt ggcaggccct gtacccgacg
    42841 atatcgcgcc gggatcggtt ttgaagtttg aatctgttaa ggagtaaaaa atgaatcagg
    42901 ttgccgttgt catcggtggt gggcaaacct taggcgcgtt cctgtgccac ggtctggctg
    42961 ccgaggggta tcgcgtcgcg gttgtcgata ttcagagcga caaagccgca aatgtggcac
    43021 aagaaattaa cgccgaatat ggtgaaggaa cagcgtatgg ttttggtgct gacgccacta
    43081 gcgagcaaag cgttctggcg ctctctcgtg gggtagatga aatctttgtt cgtgtggatt
    43141 tactggtcta cagcgccgga attgcaaaag cagcctttat cagcgacttc cagctcggcg
    43201 attttgaccg ttcgctacag gtgaatctgg tgggttattt cctgtgtgcg cgtgaatttt
    43261 cgcgtttgat gatccgcgat gggattcagg gacgcattat tcagatcaac tcgaaatccg
    43321 gcaaagtggg cagcaaacac aattctggtt atagcgccgc gaaatttggt ggcgtagggc
    43381 tgactcaatc actggcgctg gatctggcgg agtacggcat tacggtgcat tcactgatgc
    43441 tcggtaacct gctgaaatcg ccgatgttcc agtcgctgtt gccacaatat gccaccaagc
    43501 tgggtatcaa accggatcaa gtcgagcagt attacatcga caaagtaccg ctcaaacgcg
    43561 gctgcgatta tcaggatgtg ctgaatatgc tgctgttcta cgccagtcct aaggcgtcgt
    43621 actgtaccgg acagtcgatc aatgtcaccg gcggtcaggt gatgttctga tcaacagcgg
    43681 agatccgtta aggatctccg tgagactaat agaatgcctg atgcgctacg ctcatcaggc
    43741 atacaggact tccgccacta cattaaggaa aagttatggt atccgcactc atcactgtcg
    43801 ccgttatcgc ctggtgtgcg caactggcct taggcggctg gcaaatttct cgttttaacc
    43861 gtgccttcga cacactatgc cagcaagggc gggttggcgt gggccgttcc agcgggcgct
    43921 ttaaaccgcg ggtcgtggtc gccatcgccc tggacgatca gcagcgcatc gccgacacct
    43981 tgtttatgaa aggactgacc gtcttcgccc gaccgcaaaa aattcccgca attaccggta
    44041 tgcatgcggg tgatttacag cccgatgtga tctttcccca tgatccacta tcacagaatg
    44101 ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg ttgtgaatgt tacttgcttg
    44161 cgaagttatc attttgaaac ctaaatcagg taatcacgcc catgaaacct cgtcagcgtc
    44221 aagccgccat tctggagtat ctgcaaaagc agggaaaatg ctcggttgaa gaattggcgc
    44281 aatattttga caccacaggc acaactattc gcaaagatct ggtcattctg gaacatgccg
    44341 gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa agaggaatcc gatccgccta
    44401 tcgatcataa aacactcatc aacacccaca agaaagagct gatagcagaa gctgccgttg
    44461 gttttattca tgatggcgat tcgatcattc ttgatgctgg cagtaccgtt ttgcagatgg
    44521 tgcctctgct ctcgcgcttt aataacatca cggtgatgac caacagcctg catatcgtca
    44581 atgcgttatc tgaactggat aatgaacaaa ctatcctgat gccaggcgga acgtttcgta
    44641 aaaaatcggc ctcatttcac gggcagctgg cagagaatgc cttcgagcat ttcaccttcg
    44701 acaaattatt catgggtacc gacggcatcg atctcaatgc gggcgtaacc accttcaacg
    44761 aggtctatac cgtcagtaag gcaatgtgca atgccgcgcg cgaagtgatt ttgatggcgg
    44821 actcatcaaa gtttggccgt aaaagcccca acgtagtttg cagtcttgaa agcgtcgata
    44881 agctgattac cgacgcagat atcgatccgg cgtttcgtca ggcgctggaa gagaaaggga
    44941 tcgatgtgat cataaccgga gagagcaatg agtgaagcac tactgaacgc gggacgtcag
    45001 acgttaatgc tggaattgca ggaagcaagc cgtttaccgg aacgtctggg cgatgatttt
    45061 gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag tggtggtttc gggaattggc
    45121 aaatcgggcc acattggtaa gaaaatcgcc gcaacgcttg ccagtaccgg cacaccggct
    45181 ttttttgtcc atccggcaga agcgctgcat ggcgatctgg ggatgatcga aagccgcgat
    45241 gtgatgctgt ttatctctta ctccggtggg gcgaaggaac tggatctgat tattccgcgt
    45301 ctggaagaca aatctatcgc gctgctggcg atgaccggca aaccgacgtc accgctgggc
    45361 ctggcggcga aagcggtgct ggatatctcc gtagaacgcg aagcctgccc gatgcacctt
    45421 gcgccgacct ccagcaccgt caataccctg atgatgggtg acgcgctggc gatggcggtc
    45481 atgcaggcgc gcggatttaa tgaagaagat tttgcccgct cccaccctgc tggagcactg
    45541 ggcgctcgct tgctgaataa agtgcatcat ctgatgcgcc gtgacgatgc catcccacag
    45601 gtggcgttaa ccgccagcgt gatggatgct atgctggaac tcagccgcac cggtctgggg
    45661 ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg tctttaccga cggcgattta
    45721 cgtcgctggc tggttggcgg cggcgcactc acaacgccag tcaatgaagc gatgacggtc
    45781 ggcggcacca cgttgcaatc gcaaagtcgc gccatcgacg ccaaagagat cctgatgaag
    45841 cgcaaaatca ctgccgcacc ggtggtggat gaaaacggca aactcaccgg cgcaataaac
    45901 ctgcaggatt tctatcaggc cgggattatt taatccttca atcccagacg tttcgccagc
    45961 cgatgcaggt tggcgacgtc ggtttccagc atccgcgcgc aggcagccca gttgtgatga
    46021 ttttgtgcca gtgcctgacg aatagtttca cgctggaacg cttctgtcgc ttcacgcagg
    46081 ttttgcttaa caacgggcac cgccgccact tctggcggcg gcaacgtcac ctcaggaaaa
    46141 gcaaaatgtt gcgcctcaag aatcacttca tcgccgctgc gggtggctct cgccagcact
    46201 accgcccgat gaatagcatg ttccagttcg cgcacgtttc ccggaaaact gtagtgttgc
    46261 agtaaatttc gcgccccggc acttaatacc acgcgggaga gcccctgccg caaacgacac
    46321 tgctcgcaga aataccccgc cagcagaatg acatcatcgc cccgctcacg cagcggcggc
    46381 accgaaagtg gaaacacact caggcgatga aacaaatcgg cgcggaatcg ccctgccagc
    46441 acctcttcgc gtaagtcgcg gttagtcgcc gccagcacgc gcacatcgac ccgcaaacta
    46501 cggtcatcgc caacgcgctg aatatcgcca tactgcaaca ccctcagcag cttggcctgc
    46561 aatgccaacg acaactcgcc gatctcatcg agaaacagcg tgccgttatc cgccatttca
    46621 aacttcccac tgcggttact gatagcgcca gtaaacgctc ctttcacatg cccgaacaac
    46681 tcactttccg ccacactttc cggcagtgcg gcacagttga gatagaccag cggattcacc
    46741 gcccgtggcg aggcctcatg aatcgctttc gccaccagct ccttaccggt tcccgtctca
    46801 ccgctgatca ggacgttgag atcggacgcc gccacaatct caatctcttt tttcagttgc
    46861 gtcatgcctg gcgacagacc gatcatctgc gtctgtttca ccgcttcaaa tggagcggca
    46921 tcgcccggca gcatattctg gctttccagt tgttcaatca gcaacgcatt gcttaacgct
    46981 cccgccgcca gcgcggcaat caggcgtaac tcttcgtcgc tgaaaacatc gaactgatcg
    47041 ggctgcatcc cgtcgagcgt caatgcgccg atcaggtttt gcccggcaaa cagcggcaga
    47101 ccaacgcagg cgtgaacctt cagactctcc tgccctggaa tcaaaccgtc atagggatcg
    47161 ggcaattcgc tgtctgcggg aaagcgcacc acatcacccg cgcgggcaat cgcttccagc
    47221 cgtggatgac cttccagcgc aaagcgtcta ccgagtacat ccttcgccag cccgtcgatg
    47281 gcaagcggaa taaactgccg cgagtcgtaa cgtagcaacg cagacgcatc gcactccagc
    47341 acctgacgca gcgtggtgat caggcgctga aaacgatcct gatgaccaat cccacgctgc
    47401 aattcgatgg cgatattcgc cagcacatca acggaaaaac tcatctttgc ctcactgtca
    47461 atttgactat agatattgtc atatcgacca ttggattgat agtcattttg actactcatt
    47521 aatgagcata attttattta tagagtaaaa acaatcagat aaaaaactgg cacgcaatct
    47581 gcaattagca agacatcttt ttagaacacg ctgaataaat tgaggttgct atgtctattg
    47641 tggtgaaaaa taacattcat tgggttggtc aacgtgactg ggaagtacga gattttcacg
    47701 gcacggaata taaaacgctg cgcggcagca gctacaacag ctacctcatc cgcgaagaaa
    47761 aaaacgtgct gatcgacacc gtcgaccata aattcagccg cgaatttgtg cagaacctgc
    47821 gtaatgaaat cgatctggcg gatatcgatt acatcgtgat taaccatgca gaagaggacc
    47881 acgccggggc gctgaccgaa ctgatggcac aaattcccga tacgccgatc tactgtacag
    47941 ccaacgctat cgactcgata aatggtcatc accatcatcc ggagtggaat tttaatgtgg
    48001 tgaaaactgg cgacacgctg gatatcggca acggcaaaca gctcattttt gtcgaaacac
    48061 caatgctgca ctggccggac agcatgatga cttacctgac aggcgacgcg gtgctgttca
    48121 gtaacgatgc tttcggtcag cactactgcg acgagcatct gttcaacgat gaagtggatc
    48181 agacggagct ttttgagcag tgccagcgtt actacgccaa tatccttacg ccgttcagcc
    48241 gtctggtaac gccgaaaatt accgagatcc tgggctttaa cttaccagtc gatatgatag
    48301 cgacctctca cggcgtggta tggcgcgata acccgacgca aattgtcgag ctgtacctga
    48361 aatgggcggc tgattatcag gaagacagaa tcaccatttt ctacgacacc atgtcgaata
    48421 acacccgcat gatggctgac gctatcgccc aggggattgc ggaaaccgac ccacgcgtgg
    48481 cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga aatcctgacc aatgtcttcc
    48541 gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa cgtgatgatg ccgaaaatcg
    48601 ccgggctggt ggagaagatg accggattac gcttccgtaa caaacgcgcc agtgctttcg
    48661 gctctcacgg ctggagcggc ggtgcggtgg atcgtctttc cacgcgcctg caggatgcgg
    48721 gtttcgaaat gtcgcttagc ctgaaagcga aatggcgacc agaccaggac gctctggagt
    48781 tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg ctgccgcaga
    48841 gcacggtgaa tacggtagtt aaagaagaaa cctctgccac cacgacggct gacctcggcc
    48901 cacggatgca gtgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc
    48961 aggacgttgc gccaggaacg ccgtggagtg aagtcccgga taacttcctg tgcccggaat
    49021 gctccctcgg caaagacgtc tttgacgaac tggcatcgga ggcaaaatga gcaacggcat
    49081 tgtgatcatt ggttcgggct tcgccgcccg ccaactggtg aaaaatattc gcaaacagga
    49141 cgccagtatt ccattaaccc tgattgccgc cgacagtatg gatgagtaca acaaacctga
    49201 cctcagccat gttatcagtc aggggcaacg tgccgacgac cttacccgcc agacggcggg
    49261 tgaatttgcc gagcagttta atctgctcct gtttccgcaa acctgggtga cggatatcga
    49321 tgccgaagcc cgtgtggtga aaagccagaa taatcagtgg caatacgaca agctggtgct
    49381 ggcaaccggt gccagcgcct ttgtcccgcc agtgcctggg cgtgagttaa tgctgacgtt
    49441 aaatagtcag caagagtatc gcgcctgtga aacgcaactg cgggatgccc gacgcgtgtt
    49501 gattgttggc ggtggtttga ttggtagcga actggcgatg gatttttgtc gtgcaggcaa
    49561 aatggttacg ctaatcgaca acgctgccag tattctggcg tcgttaatgc caccggaagt
    49621 aagcagtcgc ttgcagcatc ggttgacgga gatgggcgtt catctgctgt tgaaatctca
    49681 gttgcagggg ctggaaaaaa cggattctgg cattctggca acgctggacc accagcgcag
    49741 catcgaagtg gatgcggtaa ttgccgccac cggactgcgc ccggaaaccg ccctggcacg
    49801 acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat agttatctgc aaaccagtaa
    49861 tgccgatatt tacgcgctgg gcgattgcgc ggaaattaac ggtcaggtat tgccgttcct
    49921 gcagccgatt caacttagcg cgatggtgct ggcaaaaaat cttctcggca ataacacgcc
    49981 gctgaaacta ccggcgatgc tggtgaaaat caaaacgccg gaattaccgc tgcatctggc
    50041 aggcgaaacc cagcgtcagg atttacgctg gcagattaat accgaacgcc agggaatggt
    50101 ggcgcgcggt gttgacgatg cagaccaact tcgcgccttt gtggtcagtg aggatcggat
    50161 gaaagaggca tttggattgt tgaaaacgtt gccggtgtag gtgggctact gtgcctaaaa
    50221 tgtcggatgc gacgctggcg cgtcttatcc gacctacggg gacgcatgtg taggccggat
    50281 aaggcgttta cgccgcatcc ggcaatggtg tccaaatgca acacgtttta tccgttctgg
    50341 acttcacccg ctaaccaacg cgccgcagca ataaccccct gccccagaga caaaccgcca
    50401 tcacccgccg gtaaactctg tggaaacagc aatgtgaaat cagcgagata atgcgccaga
    50461 cgtgcacgca gcaaacggtt atgaataacc ccgccgctaa ataccagcgt agtgatacca
    50521 cgcatcgtgg cctgctcacg catcaacgcg gcaaaaccct gcgccagcgc atcatgaaac
    50581 gcccacgcgc gttgattaac cggtgcctgc cagttcagcc actgctgcca gaaagtggcg
    50641 agatccagtt gattgtccac ccgcggcatc gtcaccggat gcgtcactcc gtcgcatgag
    50701 gctgctagcg cctccagagc acaagccgct tcaccttcat aacttaacgt ggctggcgca
    50761 cagcccagtg ccgccgccac agcatcgaac aaacgcccac acgatgacgc cagcggcgcg
    50821 ttaattccac gctcaatggc ccgcgccagt acgctccagt tttgctgttg cacacttgcc
    50881 gtttcagggt aattctgcca ctccggcaca aagcgcaggc actgcgccag caggttacgc
    50941 cacggctgct tcgccgccaa atcgccaccc ggaagcgcca ctgcaggcaa gccgcccagg
    51001 tgctcacatt cgcgatagtt cacccgcagg cactcgccgc cccacaaagc gccgttctcc
    51061 cccataccga taccgtcgag cgtcaaagca atgacatcac cgccatccag cggccactga
    51121 tgctctgcca gacacgccgc tgcgtgggca tgatgatgca gcaccgtttg cgtcggcaga
    51181 ttcatttcgc tcgcccactg gcaggagaca tagcccggat gtgcgtcatg cacaacgtat
    51241 tgcggagtga aattgtagat gttttgcatc aggcgtaacg cttcgcgcca ctgcgtctgg
    51301 atgccatcgt cacttaaatc gcccagatgc tggctcaaca ccacttgttc accgcgcacc
    51361 aggcagaagg tatttttcag atccgcgccg agacacagta caggcggaac atttttaaag
    51421 cccggaggca aagccagcgc atccggcaca tacccccgcg aacggcgcag catttcgccg
    51481 ctttcgcgca ccaccgaatc atccatccgc tgcacaatgt cgcggttgtg tattaagaat
    51541 ccgtcggcaa tgccctgcaa atcctccagc gcctgttcgt tgctgatagc cggtggttta
    51601 ccgctcaggt tgccagaggt catcaccagc gggcattgta gttcctgtaa cagcaaatgc
    51661 tgaagcggat tcgcaggcaa cattaccccg acttcgttaa ggccaggggc gatatcatca
    51721 caaagctcag gaacgtattt tttatccacc agcacaatcg gcgcggcggg cgtggtaagc
    51781 aactggcgcg cagcgtctgg taaaccgtca gccactggca acatgactgc cagcggtttc
    51841 gccgggcgat gtttgcgcgc ccgaagtgtc gccaccgcgt tactgttacg tgcatcgcag
    51901 gcaagatgaa atccgccaat ccctttgatg gcgacaatgt tgcccatttt taactgtgcg
    51961 atagccgcct gtaatgccgc ctcttgttct gcatgttcac catgacttac ccattcaaga
    52021 tacgggccac actccgggca ggccaccggc tgggcgtgga agcgacgatc gagcgggtcg
    52081 cggtactctt tatcacaggc tggacacagc ggaaacgccg ccatcacggt aaacgggcga
    52141 tcgtaaggca tggcgcgaat aatggtgaag cgcgggccgc agtgggtaca gttgataaat
    52201 ggataacgat aacgccgttc gcctggggta ttcatttcgg caaggcaagc agggcaagtg
    52261 gcggcatcgg gaacaatttg cgtattcatg gcgccgcccg cgctctggcg gatggtgaac
    52321 tcagtgggca gttgtgacca gataaacggt tcacgctcaa cgctatcaat acgcgccagc
    52381 ggcgggcagt gctgatgcaa ttgaacaaga aacgtttccg ggtcttccag cagccggact
    52441 tctacgccat cgccgtcatt acagacatcg ccgtgaagat ttaattgctg tgccagctgc
    52501 cagacaaacg gacgaaaacc gacgccctgc actttgcctc gaatacgcag ttggacaccg
    52561 caagatgtgt tttttgccat tgagttattc ccgccatcat gaattgcgta acccgtccag
    52621 ccggacacga cagcgtcgca tccggcagtc acaggtcggc gataccgccc gctccgtatt
    52681 ctacgaatat ttccgggaat tcctttgatg ccagaacagt tctgtaagat ttttagaaca
    52741 tcagcgccgt acggcggcgt ttttctgcgc tcagttgttc aagtttattg cgatcgacac
    52801 aaattagcgc atgggtcggg caagccgcca tacacgccgg gccgtcttca cgatggttgc
    52861 acaggtcgca tttattggct tcggctttgt cagcccgtac attcagccct gcgccgctgt
    52921 tgcgaatcac cggacgtacc accacttcca tcgcgccata cgggcaagcc acaacgcagg
    52981 ttttgcaacc aatgcaacgt tcctgcatca catgaacaaa ccctttatca cggctgatag
    53041 caccattcgg gcagacgtta gcgcacggtg catcttcaca ctgacggcaa actgtcgccg
    53101 tggaaatgtt cacaccttta atgacatgga tacgcggtaa aaatgtttcc ggggtcagcg
    53161 atgcacagtc ctgattttcc tgatgagaaa ccacgcacgc cacttcacag gtacggcaac
    53221 caatacattt actcgcgtca gcaatgatga aacggttcat caaattctcc agcaatgaca
    53281 gttaatgcgc cgatacattc acaaatcatg ccagttttta atttgctgtt atttaaggaa
    53341 attaatttct gtaatgcagg aaaaaagatg tcatcgacac tagtgacgat gacatgtgat
    53401 gacaatgttt atcgcgaaag agcaatgagt gagtcgcggc ggatcagttt tccgctgaag
    53461 gttttcggcg gtgagaaatc cccgccatcg agcataaaaa tcagccgtcc aataatttcc
    53521 tgaatcatct cagtcaccgg aatttttacg ctggagagcg ccggaacgat atagggggca
    53581 attgcgatat catcgaatcc gataactgac acctgctctg gcaccgctac gccgcgcttg
    53641 tgtaacgctt tcatcgcacc tatcgccata tcgtcgttac tggcaactaa cgcgctaaat
    53701 ttagccccac gttcgagcaa cgtttctacc ccttcggccc cgctggcggg cgtccattta
    53761 ccgttagcga taagtttttc attgagcgca ataccatgct gcgccagcgc gtctttatat
    53821 ccggcaagac gttcaacgct ggtgggggaa tccatcgagc cggtaagaaa agcaatctcc
    53881 tgatgcccgg cgtttatcaa ctctgccacg gcgttaaagc tggtctgttt atgatcgcac
    53941 cagacgctat ggctgctatt tttgcgcagg cggcgattaa gcaccattat cggctgactg
    54001 tgcgcgtcaa tgatgtcatc gatctcatcc acacttaaaa aacgcgggta aatcataatg
    54061 gcgtcgcagc gcaaatccag cagatactga atcgcctggc gctcttcttc ggcgctgtgt
    54121 ttaccatctg ccaatagcaa ctgccgccct ttctcttccg ccattcgcgc ggcatgaaac
    54181 agtaattcac taaaataaat gccgtggtaa agcgtgttag tcactaccag ccccagcgtc
    54241 tgggtacttt tcgccgacag attgcgcgcc agcaagtttg gacggtaacc gctctcttct
    54301 accgcctgaa acacgcgatc tttagtctcc tggctgacgt agccattacc tgaaagcacg
    54361 cgggaaacgg tcgcttttga aaccccggcg cgcttcgcca cttccagcat cgtcgtcatc
    54421 attttcatcc ctttacacgc aatcaacgca gtgtactgca ccgtttaccg attgtccttg
    54481 cgcaatcggc gggaaaaata ttcaggtgac cggtttcaca aatataaaaa atgaacaatt
    54541 aactctcttg cttatttggt tacaactatt catgattttg tgaaaccggt ttcttaattc
    54601 cgtttcagca tcggcatttt tccgtcacgt cgactgataa caactacatc ttccctactg
    54661 ataacaggat aaaatccgat ggccaaaaat tatgcggcgc tggcacgctc ggtgatagcg
    54721 gcactgggcg gcgttgataa catctcggcg gtcacgcact gtatgacgcg tttgcgcttt
    54781 gttatcaaag atgatgcgct tatcgacagc ccgacgttaa aaaccatccc cggcgtgctc
    54841 ggcgtggtac gtagtgacaa ccagtgtcag gtgattattg gcaataccgt ttcacaagcc
    54901 tttcaggaag tcgtcagcct gctgccggga gatatgcagc ccgccctgcc cgtgggtaaa
    54961 cccaaactca cgctgcgtcg cattggtgcg gggatcctcg atgcgctgat cggcaccatg
    55021 tcaccgctga tcccggcgat tatcggcgga tcgatggtca aactgctggc gatgatcctc
    55081 gagatgagcg gcgtgctgac aaaaggatcg ccgaccttaa ccattctgaa tgtgattggt
    55141 gacggtgctt tcttcttcct gccgctgatg gtcgcggctt cggccgccat caaatttaaa
    55201 accaatatgt cgctggcgat tgccatcgcg ggtgtgctgg tacatccgag ttttattgaa
    55261 ctgatggcga aagcggccca gggtgaacat gtcgaatttg ccctgattcc agtcaccgcg
    55321 gtgaaataca cctacacagt gatcccggcg ctggtgatga cctggtgcct gtcatatatc
    55381 gaacgctggg tggatcgcat tacaccggcg gtgaccaaaa acttcctcaa gccaatgctg
    55441 attgtgttga ttgccgcacc gctggcaatc ctgctgattg gcccgattgg tatctggatc
    55501 ggtagcgcca tttcggcgct ggtttacacc attcatggtt atctgggctg gctttcagtc
    55561 gccattatgg gcgcgctgtg gcctctgctg gtaatgaccg ggatgcaccg cgtctttacg
    55621 ctaaccatca ttcagaccat tgccgaaacc ggcaaagaag ggatggtcat gccgtcagag
    55681 atcggcgcta acctgtcgct gggcggttca tcactggcgg tggcgtggaa aacgaaaaac
    55741 tcggaactgc gccagactgc gctggctgcg gcggcatcag ccattatggc ggggatttcc
    55801 gaaccggcgt tatacggcgt ggcgatccgc ctgaaacgtc cgcttatcgc cagtcttatc
    55861 agcggtttta tttgcggcgc ggttgccggt atggcggggc ttgccagcca ctcaatggca
    55921 gcgccggggc tatttaccag cgtgcagttc ttcgatccgg cgaatccaat gagcatcgtc
    55981 tgggtgttcg cggtcatggc gctggcggtg gtgctgtcgt ttatcctcac actgttgctc
    56041 ggctttgagg atattcctgt tgaggaagcg gctgccgagg cgcgaaagca tcagagcgca
    56101 caaccgaccg tcgccaaaga agtaagtctt aattgaggat gaaaatgtca gtatttccag
    56161 aaggtttttt atggggcggc gcgcttgccg ccaaccagtc tgaaggtgcg ttccgtgaag
    56221 gtggcaaagg actgacaacg gtcgatatga tcccacacgg cgagcatcga atggcggtga
    56281 aactggggct ggaaaaacgt tttcagttgc gcgatgacga gttttatccc agccatgagg
    56341 cgacgggttt ttatcatcgt tataaagaag atatcgccct gatggcagag atgggattca
    56401 aagtgttccg tacctcaatt gcctggagcc gccccttccc gcagggtgat gaactgacgc
    56461 ccaaccagca gggcattgct ttttaccgtt ctgtctttga agagtgtaaa aagtacggta
    56521 tcgaaccgct ggtcacattg tgccacttcg atgtgccgat gcagctggtc accgaatatg
    56581 gctcctggcg taaccgcaag ctggtggagt ttttcagccg ctacgccagg acctgctttg
    56641 aagcatttga tggtctggtg aaatactggc taaccttcaa tgaaatcaac attatgttgc
    56701 atagcccgtt ctccggcgcg gggctggtgt ttgaagaagg tgaaaatcag gatcaggtga
    56761 aatatcaggc cgcgcatcac cagctggttg ccagtgcgct agccaccaaa atcgcccatg
    56821 aggttaaccc gcaaaatcag gtgggctgta tgctggcggg cggtaacttc tacccttaca
    56881 gttgcaagcc ggaagatgtc tgggcggcgc tggagaaaga tcgggaaaac ctgtttttta
    56941 tcgatgtgca ggcgcggggc gcgtatccgg cttactctgc tcgcgtattc cgcgaaaaag
    57001 gggtaaccat cgacaaagca ccgggcgatg atgagatcct gaaaaacacc gtcgattttg
    57061 tctctttcag ctattacgcc tcgcgctgcg cctcggcgga gatgaacacc aacaatagca
    57121 gtgcggcgaa cgtagtgaaa tcgctgcgta acccgtatct acaggtgagc gactggggct
    57181 ggggaattga tccactcggt ctgcgtatca ccatgaacat gatgtacgac cgctatcaga
    57241 agccgctgtt tctggtggaa aacggcctcg gcgcaaaaga tgaacttgct gccaatggcg
    57301 agattaacga tgactatcgc atcagctatt tacgcgaaca tatccgcgca atgggcgaag
    57361 cgattgcaga tggcattccg ctgatgggct acaccacatg gggctgtatt gatttagttt
    57421 ccgcctctac gggtgaaatg agcaaacgct acggttttgt ctttgttgac cgtgacgacg
    57481 caggcaacgg cacgctgacg cgcacacgta agaaatcgtt ctggtggtat aaaaaagtga
    57541 ttgccagtaa tggggaagat ttagagtagg taacctactg tcgcactaac caggaaggcc
    57601 cgataagcgt tgcgcatcgg gcaatctggg gtttgctatc agttctgatt ccctcatcaa
    57661 gaacatccct gtcctgatta cttattgaaa aactactctt cttccaccgc taactgcgcg
    57721 aagccgccgt tcccttccca gccttccagt cgttgataaa cggtttctac cgcatcttta
    57781 atcggctggg tcatcgggta gtaaaagccg acgatatccg gctggatgcc gaggaaaatc
    57841 acttcgccaa tatcttcttt caactgatcg ataaggtagt tcaacggcat gttatgggta
    57901 gtcatcataa acatctcggc gatatcatcc gggtcgatga tgcggatttc gccggggttt
    57961 agccccatat ccgtggcgtc gacaatcagc agccgtgtcg ggcgcagttc gcggatagcg
    58021 acaatgtcgt tttccggtgc gctaccgccg tcaatcacca cccagttacc tttcggcgcg
    58081 gcggcgcact tttccgccag cagcggacct gcgccatcat cacccatcat gctattgcca
    58141 acacagagta aaacgtcagt cacgcagtct cctcaccatc aaatagatgg cgttttcctg
    58201 atgaatatca tgcagcatac ttaacagcat tgtactccat tcctgctgct gcgaagtttg
    58261 cgctgcccgg gctttatcga atgcattggc cagcatcggc acatggttaa tgtcgatgac
    58321 gatctcacca tatttcggca caccttccat tttgcggcgg gcttcgctgc ccgcctccag
    58381 agtggcaatc catgccagat attcatccca cgggcaggtg agcgccgctt ccaggcaatc
    58441 gataacccca aggtggtgac caatcgccag gctgtaatag accacctgct gcgcctcggc
    58501 gggcgtggca tcgttctcat caataaattt acggctcagt tgactgaaca ccaccttttc
    58561 actcatcgga tacgcgcctc ttcaacaaca tgattcagat ggctgacaat ctcgttcaga
    58621 cgcggatcgt tttccgcttc cagccagcgt gcaacctgtt cttcgccctg ccctaactgt
    58681 gtaaggtaat catcggcaat ctgacgaccg taacgataac ccgccagtcg acgtgcttcg
    58741 cgatccactt tcacacgcag cggctgcacc atatcgccat gcaggatctc cgccggttgt
    58801 tcatcctgct cacccggccc acgggcgtga attttctgct ccagcaggcc gagcgccatt
    58861 gcaaagccgt acagcgtggc ggctggcgtt ggcgggcagc cagggatata aacatccacc
    58921 gggacgattt tatccgtacc gccccacacg cagtagagat cgtggaagat cccgccgctg
    58981 ttaccgcagg caccgtagga gatacaaatt ttcgggtccg gcgcggactg ccacgcacgc
    59041 agcgcagggg atcgcattgc acgggtgacc gcgccggtaa acagtaaaat atccgcatga
    59101 cgcggtgaag gaacgacttt aatgccgaag cgttctgcat cgaacagcgg cgacaaagtg
    59161 gcgaaaattt cgatttcgca gccgttgcag ccgccgcagt ccacgcggta aacgtaggca
    59221 gaacgtttga tttttttcag taacgacgcc ttcatgctgg cgatggattc atccaccgtc
    59281 atcgggaccg gaatgccgtt ggcgtcacgg gggcctaata aattgctcat cagatggcct
    59341 ctttcatatg gcgagtcagt tcaatacggt cggacggcac caggcatttc tggcgcttac
    59401 attccgggca agtctcaaag ctttcgcggt ggttttccgc gcggctgtcg ccattgtgct
    59461 taagcagcgc aatggcgtag tcgatctctt tctggacggc gaaaggacga ttgcagacgc
    59521 ggcagttgca cagcgcgaag cgggactgtt gcaggaagtc ttctttcttc cacaccgcca
    59581 gttcgtactc ttgcgacagt ttgattgccg ccgtcgggca gacttcttcg cagcgtccgc
    59641 agaagatgca gcgcccgaga ttgaactccc acgccagctc accggtggcg aggtcagttt
    59701 caaccgttaa ggcgtttgac gggcaggcat tgacgcaggc cgcgcagccg atgcactgct
    59761 gcgggttctg ctctggctta ccacggaagt ttttatcaac cgcaatcggt tccagcggat
    59821 aagacgaggt cgccgtgccg gttttgatga cttttttgat aaaggtaaac atggcgattc
    59881 cttatttcag cggcgagttt ttacgctcaa tgctgtaacg ctcgagttct ttgtacggca
    59941 ccactttgct ctttttctta cgcacatcga ccacggtcat gcggtcggta caggagtagc
    60001 aagggtccag actaccgata atcagcggcg catcagaaac ggtgttgccg cgcagcatgt
    60061 agcgcagggt cggccagttc gcgtaggtcg cggcacggca gcgccagcgg tacagcttct
    60121 ggttgtcgcc ggtcatgctc cagtggatat catcgccgcg cggcgcttcg gcaaagccca
    60181 gcgcaaaacg gtgcggaatg taggtaaagc cttccaccat cagcgggccg cccggcaggt
    60241 tatccagacc gtagtcgatc atgttcagcg cggtatagac ttcgttaata cgcactttca
    60301 gacgcgaaat aacgtcgcag ccctgctcgc tgtggacttc cattggcagc aggccataac
    60361 caacaaacgg gtgatcggcg cgggtatcgc gggcgtgacc gctggcacgg accatcgggc
    60421 cgacgttact gaagtcgcga gcgatttccg ggtccagacg accaatgccg acagtgcgct
    60481 gttccatgtt cggcgtactc agcagcacat ccaccagctc ctgcacttca cgacgcatct
    60541 gttgcgccag ttgacgggtc tggatcatgt catctttcag cagatcgcga cgaatcccgc
    60601 cgatcaggtt caggccgtag gttttacgcg caccggtaag gatctctgcc attttcatgg
    60661 aggtttcacg cacgcggaag aactgcataa agccggagtc aaagccggta aagtgacagg
    60721 cgaggccgag gttgagcaga tgcgagtgca gacgttctac ctccagcaga atggcgcgga
    60781 tcatctgcgc acgttctggc acctgaatac ccatcgcgtt ttccaccgac gtggtgtagg
    60841 cggtgctgtg agcaaaaccg cagatcccgc acacacggtc agagaggaag gtcacttcgt
    60901 tataacccat gcgggtttct gccagttttt ccatgccgcg atggacatag aacaggcggt
    60961 agtcggcgtc gataatgttt tcgccatcga cgaacagacg gaagtggccc ggttcgtcag
    61021 aagtgacgtg cagcggacca atcggcacga cgttgttttt cttgtcgccc agttcgttga
    61081 tgaactcgta ggtttcagca tcggtggtcg gtgccggacg ctgacgataa tccatgctgt
    61141 ctttacgcag cggataaagt tcatccggcc agtcatccgg cagcaccaga cgacgttcat
    61201 ccggcaaacc aaccggaatc aaaccgtaca tatcacgtac ttcacgctcg ccccacaccg
    61261 cagccggaac gcgcggcgtc acggaaggat actccggttt gttggcatca acttcgacgc
    61321 gaaccgttac ccagcactta gtccccttct ccatcgacag cacgtagtaa acggcgtaat
    61381 gaccattcag tttgcgttcg tcgttaccaa aaagcaccga cagccagccc ccctgcttgt
    61441 agtagagaaa ctccaccact tccggcagat agttcacctt tacggtgaca gtcagctgat
    61501 ctttggtctg ccaggcgtgg tccagcacga cgcccggaaa tgcctcattc agcgcggcga
    61561 gataatgttg acctaatttt tcttcagaca tgctcaaact ctctttaatc acgccgccag
    61621 caaggagacg aacgctaaaa atgcaaagcc aaacccagcc caggtaatgc gcggagtaat
    61681 atcaagacgc agacgggcca tgctgttttc gaacagcgcg ataaccagga cgccgaccac
    61741 cagtttgacg atggcaatca ccaacgccag cagcagccca ccgacggtga aggtttccat
    61801 ttgtccccac ggaataaaca ccccgacgaa catctgcagc accaccaact gtttcaggct
    61861 gataccccat ttcatgacgc caaagccgct gccgctgtac tcagagagcg gaccttcctg
    61921 caactcctgc tcggcttccg ccaggtcgaa cggcagtttg cccatttcga taaaagtggc
    61981 gaacgcacag gcacaaagcg ccagcaccag cgggatgctc tggctcagcg gccagtgata
    62041 aacggtgtcg gtgatgttgc tgatgttggt ggaactggca acctgtgcgg caacccacag
    62101 accaagcagc agcatcggtt cgaccagcac gccaagcatc gcttcacggc tcgcgccgat
    62161 agcggtaaac ggactaccgg tatccagacc agaaatggca aagaagaaac gcgcgatggc
    62221 aaagagatac agtaaggtga tcaaatcacc cagttgcggc agcggagaac cgacggtcac
    62281 caccggcagc gcagtagcga tagtcagcat gacgcccacc atcacatacg gcgtcaggcg
    62341 gaacacccag ccggaggcat ccgggccaac gctctggcga cccagcagtt tgataatgtc
    62401 gcgatactcc tgcaacacgc ccggcccgcg acggttatgc aagcgggcgc gcgccacgcg
    62461 ggtaataccg gagagcagcg gcgcaacggc aaatagcacc agcgcctgaa ttaacggata
    62521 taaaacactc attctcaggc tcctcgtgaa acaataatca ccaccagcac cgccagttca
    62581 accagcgcca tccggcggaa cagcaacgca ctgccttcgc actgccagcc cggcaccagc
    62641 gacaccggat tcagccattt gcgcagtttc agcaccggcg caaacgcctg tttcaccggc
    62701 atggcaaaac cgtgggcggt aattaccatt gatttttcat ggtcgtagcc gcacacccag
    62761 gccgcgccgc gggaacgcga tggcaaacga tcgcctttgc aaatcgccat aatgatgaat
    62821 ggcagcagcg ggcaggcaat cagcagcaac gtgatcatcg gttgagaaac ggtggtgtta
    62881 gcaggttcca gcggcagagg tacagcagca gagagcatcg gcagcagcca cggcgcagca
    62941 acaccgccaa ttacgcagca aatcgccagt gccactacgc ttacgctcat caggagcggt
    63001 gcacaggtgg cgttttcggc ttctttggta cgcggcgcgc cgaggaacgt gacgccatag
    63061 actttcgcca tacacatcac cgccagcgca ccggtaattg ccagacccac agcgagcagc
    63121 ggccccagca gacgggcaac aaacgcgcca ctattgctca gtttgaagaa ggattgatag
    63181 ataacccatt ccccggcaaa accgttcagc ggcggcagtg cagccattgc catcagcccg
    63241 actaacatag cgatggagat aaccggcatt ttcttgccaa taccaccgag tttttcgata
    63301 tcgcgatgac cggtacggaa ccagacgctc cccgccccga ggaacagtac gcttttgaac
    63361 aggctatggt taagcagatg gtacagacca ccgaccaggc caagagcaat cagcgccggt
    63421 tgttcgagcg cgatacccgt tacgccagcg cccagcccca gcaggataat gccgatattt
    63481 tccagggtgt ggtaagccag tagacgctgg atattatgtt ccatcaacgc atacagaccg
    63541 ccgacaaacg cggtgatcat accgagcacc agcagcgcga tcccccacca cagcggtgca
    63601 ttaccgccca gcagtgacag ggttaaaatg cccagcaggc caattttcat gactaccgta
    63661 gaaaacaacg cggcagctgg cgcagaggcg ttcgcatgtg cctgcggcac ccagccgtgc
    63721 agcggaataa tcccggccag caggccaaag ccaatcacac tgagcagcca gatatcggaa
    63781 ccgagcggca gctgttgcat acgcatatcc agcaggcgca gatccagcgt gccgtaacgc
    63841 tgccacagca gccagcaagc aatcgccagc agcagagtgc caagacgccc cagcgcaaac
    63901 cacagtttgc cctctttgct gttgctggtg aggaacaccg cacacaaggc catgacttcg
    63961 gccattacca cgaacatgcc gaggttgctg gcaatgacgg cgcagacggc ggcagccatc
    64021 aacatattga tctgcaagcc gttgcacttc acctgcgcgt ggcgatgcca gtcaatgttg
    64081 tagaggctga caaacagacc gcacagaccg agcgtaatca gccaaatcgc gttaagcgga
    64141 gagatttgca catcgtagct taccagcgac agcgcaccgc tcacgccaac cgcgccagtc
    64201 agtacagtga agcccgcggc tgccgtatac agactaccaa ccgcgccgcc aattccggct
    64261 atccagccgc ttagcgcctt ttgaaaagaa aagagaaatg ccagaacagc ggcggcgaca
    64321 aaccacgcca cgccgctatt gatcagggaa attgcgctca tttagcctct ccactttgag
    64381 cctgctgaaa caaggtgaga tcgccaaagt ccgtgttaaa ggtcagctca cgcttacgtt
    64441 tgctgacgcg ggcgatatcg gtgttatcca ccagatgcag ggctttagtc gggcacatcc
    64501 gcacgcaggc cggaccttgt tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc
    64561 gaatacccgg cacccagtca agcagcgtac tgatacgcgc cggagcaggc ggtgccggtg
    64621 gcgctttcgg ggtattggca tttgcaggaa tatccagcgg acggctgccg gaaaattcaa
    64681 ttgcgccaaa cgggcaggcg atgccgcaca gcttgcagct tacgcacagg ctttcattca
    64741 actgcacggc cccatcaacg cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg
    64801 catcttcaca gtggtgacag agctgcggcg cagattcttt ttcatttagc atcactctca
    64861 ggcgcggcat tgattgcagg ccgtgctggc gatgcgtctc tgaacaggcg gcctcacaag
    64921 tgtggcaacc gatacagagc gtggagtcag caattacaaa acgattcacc aggcattcct
    64981 caggtgattg tcatttttga cgaaaacatg ccgatgaaat gtcattttcg acactcatcg
    65041 acacgcccat ccccaaacag gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct
    65101 gccgggcttt gtccctttac cagttggctt aaattcaccg gcacattgtt ttcaatagcg
    65161 gtgtataagc tgtcgtaaac gccagcgatt ttttcgagat agtgttccag aacctgttcg
    65221 cgatcgcgga tgttgacctt gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca
    65281 atccgctgtt tgtcttcgcc atcttttgtt tcgacgtaat actcgatggt gtggctgtta
    65341 aagccatccg ccagggtgac gtaaatacga aaatgttcaa aaaggacgaa acagagttct
    65401 ttgtccggcg cgcagctcat ggcgtgatgc aggcgcgctt tcgataacgt gatcccctga
    65461 accagcgaat tgcagtagat gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg
    65521 taatcggctt tctcgcttat ttcccaaata gtcattgtca ggttacccgt ttaacagaga
    65581 tgccagcttt aagcattttt tgtgccaact tttaatttat tattattaaa gcgattttta
    65641 agattttggt gaatttcgtc gccgtgtcga cgtgtcattt cgacatcatc gacattattc
    65701 actgcaggga taatcaacac tggcacaatt attgcttgta gctggcaata gttaatggga
    65761 ggcgatatgc acgaaataac cctctgccaa cgggcactgg aattgatcga acagcaggcc
    65821 gcaaaacacg gcgcaaaacg cgtaactggg gtctggctca aaattggcgc attttcttgt
    65881 gtcgaaacca gctctcttgc cttttgtttt gatctggttt gccgcggcag cgtggcggaa
    65941 ggttgtaaac tgcacctcga agaacaagag gccgaatgct ggtgtgaaac atgccaacag
    66001 tatgtgacgc tactgaccca gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta
    66061 cagattgtgg cagacgacgg tttacagatt cggcggatag aaatagacca ggagtgagcg
    66121 atgtgtacaa catgcggttg cggtgaaggc aacctgtata tcgagggtga tgaacataac
    66181 cctcattccg cgtttcgtag cgcgccattt gccccggcgg cacgcccgaa gatgaaaatc
    66241 accggcatta aagcgcctga atttaccccc agccagactg aagaaggcga cctgcattac
    66301 ggtcatggcg aagcgggcac tcacgcaccg ggcatgagcc agcgtcggat gctggaagtc
    66361 gaaattgacg tgctggacaa aaataaccgt ctggctgaac gcaaccgcgc gcgctttgct
    66421 gcctgcaagc aactggtgct caacctggtt tccagcccag gttccggtaa aaccaccctg
    66481 ctgacggaaa ccttaatgcg cctgaaagac agcgttccgt gcgcggttat tgaaggtgac
    66541 cagcaaaccg tgaacgatgc cgcacgcatt cgcgctaccg gcacaccagc gattcaggtg
    66601 aacaccggta aaggctgcca tcttgacgca cagatgattg ccgacgccgc accgcgtctg
    66661 ccactggacg ataacggtat tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc
    66721 agcttcgatc tcggtgaaaa acacaaagtg gcggtgcttt ccgttaccga aggtgaagac
    66781 aaaccgctga aatatccgca tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt
    66841 gacctgttgc cgtatctcaa ctttgacgtt gagaagtgca tcgcctgcgc ccgcgaagtc
    66901 aatccagaaa ttgaaatcat ccttatttcc gccaccagcg gcgaagggat ggaccagtgg
    66961 ctgaactggc tggagacaca gcgatgtgca taggcgttcc cggccagatc cgcaccattg
    67021 acggtaacca ggcgaaagtc gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag
    67081 tcggcagctg cgatgaaaac ggtcagccgc gcgtgggcca gtgggtactg gtacacgttg
    67141 gctttgccat gagcgtaatt aatgaagccg aagcacgcga cactctcgac gccttgcaaa
    67201 acatgtttga cgttgagccg gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg
    67261 ttttgttgat gaatatcgcg cgccggaaca ggtgatgcag ttaattgagc atctgcgcga
    67321 acgtgcttca catctctctt acaccgccga acgccctctg cggattatgg aagtgtgcgg
    67381 tggtcatacc cacgccattt ttaaattcgg cctcgaccag ttactgcctg aaaacgttga
    67441 gtttatccac ggtccgggtt gcccggtgtg cgtactgccg atgggcagaa tcgacacctg
    67501 cgtggagatt gccagccatc cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt
    67561 gccggggaaa cagggatcgc tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt
    67621 ctattcgccg atggatgcgt tgaaactggc gcaggagaat ccaacccgca aagtggtgtt
    67681 cttcggctta ggttttgaaa ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa
    67741 agcgcgtgat gtgcagaatt tttacttctt ctgccagcat attacgctta tcccgacgct
    67801 gcgcagtttg ctggaacagc cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt
    67861 tagtatggtt atcggcactg atgcctataa ttttatcgcc agcgattttc agcgtccgct
    67921 ggtggtggct ggtttcgaac cccttgatct actgcaaggc gtggtcatgc tggtggagca
    67981 gaaaatagcg gcccacagca aggtagagaa tcagtatcgt cgggtggtac cggatgccgg
    68041 taacctgctg gcgcaacagg cgattgccga tgtgttctgt gtcaacggcg acagcgaatg
    68101 gcgcggctta ggcgtgattg aatcttctgg cgtgcacctg acgccggatt atcaacgatt
    68161 cgatgccgaa gcacatttcc gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcgcg
    68221 ttgtggcgaa gtcttgacgg gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac
    68281 ctgtaatcct caaaccgcgt ttggtgcgct gatggtttcc tccgaaggag cgtgcgccgc
    68341 gtggtatcag tatcgtcagc aggagagtga agcgtgaata atatccaact cgcccacggt
    68401 agcggcggcc aggcgatgca gcaattaatc aacagcctgt ttatggaagc ctttgccaac
    68461 ccgtggctgg cagagcagga agatcaggca cgtcttgatc tggcgcagct ggtagcggaa
    68521 ggcgaccgtc tggcgttctc caccgacagt tacgttattg acccgctgtt cttccctggc
    68581 ggtaatatcg gcaagctggc gatttgcggc accgcgaatg acgttgcggt cagtggcgct
    68641 attccgcgct atctctcctg tggctttatc ctcgaagaag gattgccgat ggagacactg
    68701 aaagccgtag tgaccagcat ggcagaaacc gcccgcacgg caggcattgc catcgttact
    68761 ggcgatacta aagtggtgca gcgcggcgcg gcagataaac tgtttatcaa caccgcgggc
    68821 atgggcgcaa ttccgacgaa tattcactgg ggcgcacaga cgctaaccgc aggcgatata
    68881 ttgctggtta gcggtacact cggcgaccac ggggcgacta tccttaacct gcgtgagcag
    68941 ctggggctgg atggcgaact ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg
    69001 ctgcgtgaca ttcccggcgt gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg
    69061 gtggttcatg agttcgcggc agcctgcggt tgcggtattg aaatttctga atcagcgctg
    69121 ccggttaaac ctgccgtgcg cggcgtttgc gaattgctgg gactggacgc cctgaacttt
    69181 gccaacgaag gcaaactggt gatcgccgtt gaacgcaacg cggcagagca agtgctggca
    69241 gcgttacatt cccatccact ggggaaagac gcggcgctga ttggtgaagt ggttgaacgt
    69301 aaaggtgttc gtcttgccgg tctgtatggc gtgaaacgaa ccctcgattt accacacgcc
    69361 gaaccgcttc cgcgtatatg ctaataaaat tctaaatctc ctatagttag tcaatgacct
    69421 tttgcaccgc tttgcggtgc tttcctggaa gaacaaaatg tcatatacac cgatgagtga
    69481 tctcggacag caagggttgt tcgacatcac tcggacacta ttgcagcagc ccgatctggc
    69541 ctcgctgtgt gaggctcttt cgcaactggt aaagcgttct gcgctcgccg acaacgcggc
    69601 tattgtgttg tggcaagcgc agactcaacg tgcgtcttat tacgcatcgc gtgaaaaaga
    69661 cacccccatt aaatatgaag acgaaactgt tctggcacac ggtccggtac gcagcatttt
    69721 gtcgcgccct gatacgttgc attgcagtta cgaagaattt tgtgaaacct ggccgcagct
    69781 ggccgcaggt gggctatacc caaaatttgg tcactattgc ctgatgccac tggcggcgga
    69841 agggcatatt tttggtggct gtgaatttat tcgttatgac gatcgcccct ggagcgaaaa
    69901 agagttcaat cgtctgcaaa catttacgca gatcgtttct gtcgtcaccg aacaaatcca
    69961 gagtcgcgtc gttaacaatg tcgactatga gttgttatgc cgggaacgcg ataacttccg
    70021 catcctggtc gccatcacca acgcggtgct ttcccgcctg gatatggacg aactggtcag
    70081 cgaagtcgcc aaagaaatcc attactattt cgatattgac gatatcagta tcgtcttacg
    70141 cagccaccgt aaaaacaaac tcaacatcta ctccactcac tatcttgata aacagcatcc
    70201 cgcccacgaa cagagcgaag tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag
    70261 taaagagatg ctgctgatta atctccacga gcgggatgat ttagccccct atgaacgcat
    70321 gttgttcgat acctggggca accagattca aaccttgtgc ctgttaccgc tgatgtctgg
    70381 cgacaccatg ctgggcgtgc tgaaactggc gcaatgtgaa gagaaagtgt ttaccactac
    70441 caatctgaat ttactgcgcc agattgccga acgtgtggca atcgctgtcg ataacgccct
    70501 cgcctatcag gaaatccatc gtctgaaaga acggctggtt gatgaaaacc tcgccctgac
    70561 cgagcagctc aacaatgttg atagtgaatt tggcgagatt attggccgca gcgaagccat
    70621 gtacagcgtg cttaaacaag ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct
    70681 cggtgaaact ggcacgggta aagagctgat tgcccgtgct atccataatc tcagtgggcg
    70741 taataatcgc cgcatggtca aaatgaactg cgcggcgatg cctgccggat tgctggagag
    70801 cgatctgttt ggtcatgagc gtggggcttt taccggtgcc agcgcccagc gtatcggtcg
    70861 ttttgaactg gcggataaaa gctccctgtt cctcgacgaa gtgggcgata tgccactgga
    70921 gttacagccg aagttgctgc gtgtattgca ggaacaggag tttgaacgcc tcggcagcaa
    70981 caaaatcatt cagacggacg tgcgtctaat cgccgcgact aaccgcgatc tgaaaaaaat
    71041 ggtcgccgac cgtgagttcc gtagcgatct ctattaccgc ctgaacgtat tcccgattca
    71101 cctgccgcca ctacgcgagc gtccggaaga tattccgctg ctggcgaaag cctttacctt
    71161 caaaattgcc cgtcgtctgg ggcgcaatat cgacagcatt cctgccgaga cgttgcgcac
    71221 cttgagcaat atggagtggc cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc
    71281 ggtattgcta acacgcggca acgtgctgca gctgtcattg ccagatattg ctttaccgga
    71341 gcctgaaacg ccgcctgccg caacggttgt cgctcaggag ggcgaagatg aatatcagtt
    71401 gattgttcgc gtgctgaaag aaactaacgg cgtggttgcc gggcctaaag gcgctgcgca
    71461 acgtctgggg ctgaaacgca cgaccctgct gtcacggatg aagcgactgg gaattgataa
    71521 atcggcattg atttaactgc aaattgccgg acagatctgc ctgtccggca tactattcac
    71581 gaggtttttt cggacgatat ttttccggca gttctggcac cggacacttg tcatcgatga
    71641 gatgacgcac ggttaagatc ggatgacgcc acagcattct cggcccggcc caacgcataa
    71701 tctgtttcat ctcttcacgc tttgcaggct ggtaacagtg caccggacac tgcttacagg
    71761 ctggtttctc ttcgccgaac acacatttat ccagccgctt ttgcgcgtaa acaaacaacg
    71821 cctcgtaatg ctccggctcc gctgacgcct gcgggcattt cgcttgataa agatcgatca
    71881 tttttttaat cgtcagtttt tcacgagaga tacgcttgcc ggacatactg cctccacctc
    71941 attaagatgc atttatatta caacttaatc ttaaagggca ctatgactct aaagaagaag
    72001 ggttagccaa ccgatacaat tttgcgtact tgcttcataa gcatcacgca aaagctgcaa
    72061 aacagcatct ttcccggaac cagcatcaag aactcgccgt tcgcttcttc ccctgaaatg
    72121 attaactccg gtatcatgtg cgccttatgt gattacaacg aaaataaaaa ccatcacacc
    72181 ccatttaata tcagggaacc ggacataacc ccatgagtgc aatagaaaat ttcgacgccc
    72241 atacgcccat gatgcagcag tatctcaagc tgaaagccca gcatcccgag atcctgctgt
    72301 tttaccggat gggtgatttt tatgaactgt tttatgacga cgcaaaacgc gcgtcgcaac
    72361 tgctggatat ttcactgacc aaacgcggtg cttcggcggg agagccgatc ccgatggcgg
    72421 ggattcccta ccatgcggtg gaaaactacc tcgccaaact ggtgaatcag ggcgagtccg
    72481 ttgccatctg cgaacaaatt ggcgatccgg cgaccagcaa aggtccggtt gagcgcaaag
    72541 ttgtgcgtat cgttacgcca ggcaccatca gcgatgaagc cctgttgcag gagcgtcagg
    72601 acaacctgct ggcggctatc tggcaggaca gcaaaggttt cggctacgcg acgctggata
    72661 tcagttccgg tcgttttcgc ctgagcgaac cggctgaccg ggaaacgatg gcggcagaac
    72721 tgcaacgcac taatcctgcg gaactgctgt atgcagaaga ttttgctgaa atgtcgttaa
    72781 ttgaaggccg tcgcggcctg cgccgtcgcc cgctgtggga gtttgaaatc gacaccgcgc
    72841 gccagcagtt gaatctgcaa tttgggaccc gcgatctggt cggttttggc gtcgagaacg
    72901 cgccgcgcgg actttgtgct gccggttgtc tgttgcagta tgcgaaagat acccaacgta
    72961 cgactctgcc gcatattcgt tccatcacca tggaacgtga gcaggacagc atcattatgg
    73021 atgccgcgac gcgtcgtaat ctggaaatca cccagaacct ggcgggtggt gcggaaaata
    73081 cgctggcttc tgtgctcgac tgcaccgtca cgccgatggg cagccgtatg ctgaaacgct
    73141 ggctgcatat gccagtgcgc gatacccgcg tgttgcttga gcgccagcaa actattggcg
    73201 cattgcagga tttcaccgcc gagttgcagc cggtactacg tcaggtcggc gacctggaac
    73261 gtattctggc gcgtctggcg ttgcgtaccg ctcgcccacg cgatctggcc cgtatgcgtc
    73321 acgctttcca gcaactgccg gagctgcgtg cgcagttaga aactgttgat agtgcaccag
    73381 tacaggcgct acgtgagaag atgggcgagt ttgccgagct gcgcgatctg ctggagcgag
    73441 caatcatcga cacaccgccg gtgctggtac gcgacggtgg tgttatcgca tcaggctata
    73501 acgaagagct ggatgagtgg cgcgcgctgg ctgacggcgc gaccgattat ctggagcgtc
    73561 tggaagtccg cgagcgtgaa cgtaccggcc tggacacgct aaaagttggc tttaatgcgg
    73621 tgcacggcta ctacattcaa atcagccgtg ggcaaagcca tctggcacct atcaactata
    73681 tgcgtcgcca gacgctgaaa aacgccgagc gctacatcat tccagagcta aaagagtacg
    73741 aagataaagt cctcacttca aaaggcaaag cactggctct ggaaaaacag ctttatgaag
    73801 agctgttcga cctgctgttg ccgcatctgg aagcgttgca acagagcgcg agcgcgctgg
    73861 cggaactcga cgtgctggtg aacctggcgg aacgggccta taccctgaac tacacctgcc
    73921 cgaccttcat tgataaaccg ggcattcgca ttaccgaagg ccgccatccg gtggttgaac
    73981 aggtgctgaa cgagccattt atcgccaacc cgctgaatct gtcaccgcag cgccggatgt
    74041 tgattattac cggtccgaac atgggcggta aaagtaccta tatgcgccag accgcactga
    74101 ttgcgctgat ggcctatatc ggcagctacg taccggcgca aaaagtcgag attggcccga
    74161 ttgaccgtat ctttacccgc gtaggggcag cggatgatct ggcttccggg cgttcaacct
    74221 ttatggtgga gatgaccgaa accgctaata ttctgcataa cgccaccgag tacagtctgg
    74281 tgctgatgga tgagattggg cgcggaacgt ccacttacga tggtctgtcg ctggcgtggg
    74341 cgtgcgcgga aaatctggcg aataagatta aggcgttgac gctgtttgcc acccactatt
    74401 tcgagctgac ccagttaccg gagaaaatgg aaggcgtcgc caacgtgcat ctcgatgcac
    74461 tggagcacgg cgacaccatt gcctttatgc atagcgtgca ggatggcgcg gcgagcaaaa
    74521 gctacggcct ggcggttgca gctctggccg gcgtgccaaa agaggttatt aagcgcgcac
    74581 ggcaaaaact gcgtgagctg gaaagcattt cgccgaacgc cgccgctacg caagtggatg
    74641 gtacgcaaat gtctttgctg tccgtaccgg aagaaacctc gcctgcagtc gaggcactgg
    74701 aaaacctcga tccggattca ctcaccccgc gtcaggcgct ggaatggatt tatcgcctga
    74761 agagtctggt gtaataataa ttcccgatag tcttttgcta tcgggaatat taacgataac
    74821 tgacgaatca aataaaaata ccctgtataa taggaaagct tattttacag ggtaaaacca
    74881 tgccatctac acgctatcaa aaaatcaata cccatcacta tcgccatata tgggtcgttg
    74941 gtgatattca tggtgaatat cagttattac aatcccgctt acatcaactc tcttttttcc
    75001 ccgaaaccga cttacttatt tctgtcggcg ataatattga tcgtgggccg gagagtctta
    75061 acgtcctgcg cctgctaaac cagccctggt ttatctccgt taaaggcaac cacgaagcaa
    75121 tggcgctgga tgcgttcgcg accggcgatg gcaatatgtg gcttgccagc ggtggtgact
    75181 ggtttttcga tttaaatgat tcagagcaaa aagaagctac agatctgttg ctgaaattcc
    75241 atcaccttcc acatattatt gaaatcacta acgacaacat aaaatatgtc atcgcacatg
    75301 cagattatcc ggggagtgaa tatctctttg gtaaagaaat agcggagagc gaattactct
    75361 ggcctgttga tcgtgtgcag aaatcgctta atggcgagtt acaacaaata aacggcgctg
    75421 attattttat atttggacat atgatgtttg ataacattca gacgttcgct aaccagattt
    75481 atattgatac cggatcgccg aaaagcgggc ggctgtcatt ttataaaata aagtaatgag
    75541 gccgggtaaa gcaaagccac tacccggcac tttttattag cgcttacctt ccgccagcag
    75601 cggcgggatg ctcggcacca ttggcgcgtc atcaatatct ttctgcgtca tgcggaacgc
    75661 ttcgggataa tgttcgcggc tggtacgacg cagcggttcg gtatcgcgcc aggtataaag
    75721 gcaatgctgg cactgatata ccgtccagac atctttcacc ggcgatttcg ccatcacttc
    75781 aatctgttca tcggcacaac gtggacaaat catcttgttc tccttattta cgtgcggcca
    75841 gcatagcggt cagtttttca gcccaggctt tggtttccgg caaatccacc accggctggc
    75901 tgtagtgacc acggttgtcc ggggcgacag gcgtggtggc gtcgataatc agcttgtcgg
    75961 tgatccccgc cgggcttgag ccagggtcga gttccagcac ggacatattc ggcaactgca
    76021 ccaaatcccc tgccggattt actttcgagg agagcgccca catcacctgc ggcaggttga
    76081 acgggtcaac gtcttcatcg accataatca ccatctttac gtagcccaga ccgtgcggcg
    76141 tggtcatcgc acgcaggccc accgcgcggg caaagccgcc gtagcgtttt ttggtggaga
    76201 taatcgccag caggccgtgg gtgtacatgg cgtttaccgc ctgcacttcc gggaactcgg
    76261 ctttcagttg ttgatacagc ggcacacagg tggctggccc catcaggtag tcgatttcgg
    76321 tccacggcat gccgaggtac agcgattcga aaatcggcct ggtgcggtaa gagactttat
    76381 cgatacgcac cacggtcatg ttacgcccgc cggagtagtg cccggtaaac tcaccgaacg
    76441 gcccttcgat ttcgcgtttg cggctttcga taaccccttc gaggatcact tctgaacccc
    76501 acggcacatc aaaaccagtc aatggcgcgg tggcgatcgg gtacgggctt tcgcgcagcg
    76561 cgcctgccat ttcatactca gactgatcgt atttcagcgg cgtggcgccc ataagggtga
    76621 tgatcggatc gttacccagg gtgatggcaa tcggcagatc ttcaccgcgc tcttccgctt
    76681 tatgcagatg cagggcgata tcgtgcatcg gcaccggttg caggccgagc ttacgcttgc
    76741 ccttcacttc catgcggtag ataccgacgt tctgcttgcc gaagttatcc gggtcgagcg
    76801 gatcgcggga aaccacgcac gctttgtcga gatagaaacc gccatcaccg tcgtttaaac
    76861 gaaacagcgg caggatatcg aacagattaa tctcgtcacc atcgacggtg ttctgcgccc
    76921 aggctggatt ggcgcggcgc tccggggcga tcgggaagtt atcccagcgg cggataaact
    76981 catcaatctg ttttttaacc ggggtgtttg gcggcaggcc caaggaaatc gcgtggttct
    77041 gccaggaacc gatggtattc atcgccacgc gggcatcggt aaaaccgcga atattatcaa
    77101 accacagcgc cggtgcacca tcgccgatac gcccggtggc gttggcagcg gcagccagat
    77161 ccggttccgc gttcacctct tcgctgattt tcagtaactg gccgtggtca tcaagcgcct
    77221 gtaaaaagct gcgtaaatca tcaaatgcca ttattcattc tcctgagaaa aattccgggc
    77281 ctgcggcaat ccttgccagc gcctggcgta gggatgttca aggccaaatt gatccagcac
    77341 gcgggctacc acgtggtgga caatgtcatc taccgtttcg ggatggttat aaaaggcagg
    77401 catcggcggc accatcgcca cgcccatgcg tgaaagtgcg agcatatttt cgagatggat
    77461 ggtgctaagc ggcatttcac gcggcaccag caccagtttg cggccttctt taagcacgac
    77521 gtccgccgcg cgccctacca ggccatcagc gtaaccagcg cggataccgg cgagcgtttt
    77581 catactgcac ggaataacga tcatgccgtc tgtacgaaag gaacctgagg agatggtcgc
    77641 cgcctgatcc gccgggttat ggctgaagtc agcgagggca gcaacatcgc gggcgctgta
    77701 aggcgtttcc agttcaatgg tggttttcgc ccacttcgac atcaccagat gagtctcgac
    77761 attcggcatc tcccgcagcg cttgcagtaa tgccacacca agaggcgcac cggtagcccc
    77821 tgtcatcccg acgatcagtt tcattgctta ctctcttaat tgttcgtata cgaacattat
    77881 tattaaaata cgcctctgtt ctcattgcgg caagcactga aaagtaacaa catccccttc
    77941 gtcactgaaa aaccgctatg atagcggcag atagtttgcc cggagtttac atgacgttac
    78001 gaaataaagc gttccatcag ttacgacagc tttttcagca gcacacggct cgctggcagc
    78061 acgagttacc tgacctgacc aaaccacagt atgcggtgat gcgcgccatt gctgataagc
    78121 ctggtatcga acaggtggca ctgatagaag cagcagtcag caccaaagca acgttggcag
    78181 aaatgttggc aagaatggag aatcgtggct tagtcagacg tgaacatgat gccgctgaca
    78241 agcggcgtcg ctttgtctgg ctgactgccg aaggagaaaa gatacttgct gcggcgatac
    78301 cgattggtga cagcgtggat gaggaatttt tggggcgttt gagtgcagaa gagcaggagc
    78361 tgtttgtgca gctggtgcgc aagatgatga acacatagga tgcaaattgc cgggtaggac
    78421 gctgacgtgt cttatccagg cgacaaaaac acaagcttcc caaacaaaag aaaaaggcca
    78481 gcctcgcttg agactggcct ttctgacaga tgcttactta ctcgcggaac agcgcttcga
    78541 tattcagccc ctgcgtttgc aggatttcgc gcaaacggcg caggccttca acctgaatct
    78601 ggcgaacacg ttcacgggtg aggccaattt cacgacctac atcttccagt gttgccgctt
    78661 cgtaccccag caaaccgaat cgacgtgcca gtacttcacg ctgtttggcg ttcagctcga
    78721 acagccattt gacgatgctc tgcttcatat cgtcatcttg cgtggtatct tccggaccgt
    78781 tctctttttc atcggccagg atgtccagca acgctttttc ggaatcacca cccagcgggg
    78841 tgtctaccga ggtaatgcgc tcgttaagac gaagcatacg gctgacgtca tcaactggct
    78901 tatccagttg ctctgcgatc tcttccgcac ttggttcatg gtccagctta tgggacaact
    78961 cacgtgcggt tcgcaggtaa acgttcagct cctttacgat gtgaatcggc aaacgaatag
    79021 tacgggtttg gttcataatc gcccgttcaa tcgtctggcg aatccaccag gttgcgtatg
    79081 ttgagaagcg gaaaccacgt tccgggtcaa acttctctac cgcacggatc agccccaggt
    79141 tgccctcttc gataaggtcc agcaacgcca gaccacgatt gccataacgg cgggcaattt
    79201 ttaccaccag acgcaagtta ctctcgatca tccggcggcg agaggcgaca tctccacgca
    79261 gtgcgcgacg cgcaaaataa acttcttctt cggccgttaa cagtggtgaa taaccaatct
    79321 caccaaggta aagctgagtc gcgtccaaca cacgctgtgt ggctccctgc gataacagtt
    79381 cctcttcggc caaatcgtta tcactgggtt cctcttctac taaggccttt tcgtcaaaaa
    79441 cctcaactcc gttctcatca aattccgcat cttcatttaa atcatgaact ttcagcgtat
    79501 tctgactcat aaggtggctc ctacccgtga tcccttgacg gaacattcaa gcaaaagcct
    79561 ggttccgccg atttatcgct gcggcaaata acgcagcggg tttacggatt tccccttgta
    79621 acgaatttca aaatgcaagc gtgttgaact ggttccggtg ctacccatgg ttgctatttt
    79681 ttgccccgcc ttcacttctt gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc
    79741 actcaggtaa tcatcattat gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt
    79801 accggcataa acaacacggc catctgcggt cgcgataatt gcctgtcctt tgctgcctgc
    79861 gatatcaatc cccttgttgc ccccctcaga agcgccaaag gtttcgatca ctttgccctc
    79921 agtcggccag cgccaggtgg agataggcgt actggttgat gtactgctga cagtcggctc
    79981 ggttgtgctt gctgttggta ccgttacagg cgctgtgacc gtggtcgcag ttggcttgtt
    80041 gttcggcaac attttgttag cactctgttc acccgaagac tcagaatacg taattgtcgg
    80101 ttgcgacgca acagcaacgg tggaattttg tgcaggcttg atcacaactc cttgctctgc
    80161 tgcgtcggcc tgggtaatgg catttccgcc agtgattggc gtaccggaag cattacccac
    80221 ctgtaaggtc tgaccgacgt tcagcgcgta tggtgcctga atattgttgc gctgagcaag
    80281 gtcacggaaa tcgttgccag taatccaggc gatatagaaa agcgtgtcgc cttttttcac
    80341 ggtataggta ctgccgctat aactgccttt cggaatgttc ccatactgac ggttatagac
    80401 gatgcgtccg ttttccatct gtaccggctg ctgagctact ggctgcactg gctggatttg
    80461 cggttgctgc gctgtagacg tcgtccccat tttcggcggc ggcgtaatca acataccaga
    80521 attggtatgt gcaggcgcat tgccattaac ggagctgacc ggtgccggtg gatttgaagt
    80581 gtcagaacag cctgccagcc atagcgaaac cagtgacaaa gccgcaatgc ggcgaacggt
    80641 gaattttggg cttcccgcgc tcatttatcc cccaggaaaa attggttaat aaccagtgac
    80701 ataattaccg tgcaaggcac catactgaac actggaaaag atgttcacga tacgctgacc
    80761 tgcggcaaaa taaccaggaa aaatccaggt atttcctcac gttttaagcc agctcaccct
    80821 tcactaaagg gacaaagcgc acggcctcca cggtatcgat aataaattcg cctccccgac
    80881 gacgcacccg tttcaaatac tggtgctcct cccctacggg taagacgaga atcccgcctt
    80941 cgtccagctg cgtcattagc gcagttggaa tttccggcgg tgccgccgta acaatgatag
    81001 cgtcaaacgg cgcacgtgcc tgccaacctt gccatccatc gccatgacgg gttgaaacat
    81061 tatgtaaatc aagatttttc aggcggcgac gcgcctgcca ctgcaagcct ttaatccgtt
    81121 caaccgagca aacatgctgg acaagatgcg ccaggattgc cgtttgatat cccgaaccgg
    81181 tgccaatttc cagcacccgc gactgcggcg tcagctcgag taattcggtc attcgcgcca
    81241 ccatatacgg ctgcgaaatc gtctgcccct gacctatcgg caaggcgata ttgtcccagg
    81301 ctttttgttc aaacgcttca tcaacgaatt tttcacgcgg cacggcggca agtgcattca
    81361 gcacctgctc atcctgaata ccttgcgcac gtaattgatc cagaagtgct tgtacgcgtc
    81421 tgcttaccat tgcgtgccaa ctcccacgct gtttaaccag tctgaaacca catcttgcgc
    81481 gctatgcgca gttaaatcca catgcagcgg cgtgatggag acatagccct catctaccgc
    81541 agcaaaatcg gtccccggac cggcatcaca tttaccgccc ggcgggccaa tccagtacag
    81601 cgtattgccg cgcggatctt gctgcgggat cacctgatct gccggatgtc gtgtaccgca
    81661 gcgcgtcacg cgaatacctt tgatttgatc caagggtaaa tccggaacat taatattaag
    81721 aatacgcccg gtgcgcagcg gctctttaca cagtgcgcgc aaaattgaac aggttaccgc
    81781 cgcggcagtg tcgtaatgtt tatgcccgtc aagcgagacg gcaagcgccg gaaaacctaa
    81841 atgacggcct tccatcgcgg cggctaccgt accggaataa ataacatcat cccccagatt
    81901 cggcccggcg ttaattccgg acacaacaat gtccgggcgc ggacgcatca gagcattcac
    81961 gccaagatag acgcaatcgg tcggggttcc catttgcaca gcaatatcac cattttcaaa
    82021 ggtaaacgtg cgcagggagg attccagtgt cagagaattt gaagcgccgc tgcggttacg
    82081 atcgggggcg accacctgaa cgtcagcaaa ctcacgcaag gctttcgcca gcgtttgtat
    82141 accgggtgca tgtaccccgt catcattact cagcaatatg cgcataatca cctgttgtgt
    82201 tgataagttc cctgacaacg ctggttgcaa aactacccgc cggaagccag aaacggattt
    82261 ctacggtgac gtcatcccac caattccagc ttaattgttg cggatacagc agcatcgctc
    82321 tgcgcgcggc ttcaactttt tcgcgcacca gtaaagcttg taattcagtt tctgcggcga
    82381 cagctgcctg ttcgaatgcc agcgcttcac gctgagttcc ccattcacca ctgcccggca
    82441 atgcggcggt tatcatcagc tctttatcgt tgacgcgacg ctgtaattcc accagttctt
    82501 cggtggttgc aacaaaccag ctaccacgtc cggctaattg tagcgcatcg ccgtcaacaa
    82561 cttgattaac gtctgctttt ttgaggcgct cagcaacaat ctgattaaac aacgcactgc
    82621 gggctgccga caaccaaaaa ctccgtttat tgcgatcgcg caccggagta ttggtttgcg
    82681 cccagcgcag cgcgccctgc aagttgctac cgccaatccc aaaacgttgg gcaccgaagt
    82741 agttcggtac accttttacg caaatatcga tcagacgttg ttcaacgtca tcgcgattgc
    82801 tcacttcgcg cagaaccagg gtaaaggcgt tacctttcag cgcccctaaa cgcagcttgc
    82861 gcttgtgccg cgcatactcc agcacctggc agccttccaa ttgaaaggcg ctcagatcgg
    82921 gcatttcctt gcccggcacg cgagcgcata accactgttc tgtaacagca tgtttgtctt
    82981 tttgcccagc aaagctgact tcacgggcat gaattttcag gaatttcgcc agtgcatccg
    83041 ccacaaaacg ggtattgcag ccgtttttga ggattctaac cagaatatgc tcaccttcac
    83101 catcaggctc aaagcccaaa tcttccacca ccacaaagtc ttccggattg gctttcagca
    83161 gcccggtgcc ttgcggttta ccgtggaggt aagtgagatt atcaaactca atcattttgt
    83221 tgccttaatg agtagcgcca ccgcttcaca ggcaatccct tccccacgtc cggtaaatcc
    83281 aagtttttcc gtagtagtgg ctttcacgtt aacatcatcc atatggcagc cgagatcttc
    83341 ggcaataaac acgcgcattt gtggaatgtg cggcaacatc ttcggtgcct gagcgatgat
    83401 agtgacatcg acgttgccaa gggtataacc cttcgcctga atacgacgcc aggcttcgcg
    83461 tagcagctcg cggctatcgg cacctttaaa tgccggatcg gtatccggga acagtttgcc
    83521 gatatccccc agcgccgccg cgccaagcaa tgcatcggtc aacgcatgga gcgccacgtc
    83581 gccatcagaa tgcgccagca atcctttttc gtaaggaatg cgtacgccac caatgataat
    83641 tgggccttca ccgccaaagg cgtgtacgtc aaaaccgtgt ccaattcgca ttatgtattc
    83701 tcctgatgga tggttcgggt gaggtaaaac tcggccagtg ccaaatcctc cgggcgcgtg
    83761 actttaatgt tatccgcacg gccttcgacc aactgaggat ggaatccgca atattccagc
    83821 gccgaggctt cgtcggtaat agccgcgcct tcatttagag cgcgcgtcag acagtcatgt
    83881 aacagctcac gagggaaaaa ttgcggcgtc agcgcgtgcc ataagccgtt gcgatcaacg
    83941 gtatgagcaa tggcattttt gcccggttcg gcacgtttca tagtatcgcg cactggtgcg
    84001 gctaggatcc ctcccgtgcg gctggtttcg ctcaacgcca acaatcgcgc gaggtcatcc
    84061 tgatgcagac aaggacgagc ggcgtcatgc accaataccc actgcgcgtc gccagcggct
    84121 ttcaaacctg ccagcacgga atcggcacgc tcatcaccgc catctacaac ggtgatttgc
    84181 ggatgattcg ccagaggaag ttgtgcaaaa cggctatcgc caggacttat ggcaatgacg
    84241 acacgtttca cccggggatg cgccagcagc gcatacaccg agtgttcaag aatggtttga
    84301 ttaccgattg agagatattg cttaggacat tccgtttgca ttcgacggcc aaatccggcc
    84361 gccggaacca cggcgcaaac atccaaatga gtggttgcca tgttaattcc cgggctgatt
    84421 tatcgattgt tttgccccgc agactgtgcg cgcttcgacg cgtcaggcac cagacgataa
    84481 aaagtttcgc ccggcctggt catgctgagt tcattacgcg cacgctcttc gagcgcctcc
    84541 tggccgccat tgagatcgtc aatttcggca aaaagttgat cgtttcgcgc tttaagtttc
    84601 gcgtttgtag cttgctgtgc cgccacatca tcattgacgc gggtatagtc atgtataccg
    84661 ttcttaccga accacagcga atactgtagc cagaccagaa tagccagcaa cagcagcgtt
    84721 agtttaccca tcctgccccc tgaaaaacgg catcatcatc ccatgcatcc gaagacgact
    84781 ctacatcctc tgttggggat accgcgacaa cgcgggcaaa tgtaccacat ttgtccattg
    84841 ttacgtatac ccagggcgtg cagaacataa tctcattatt agttacggtt tgaattatga
    84901 acagaggaga caagaaagta caaattagcc cagtagccac ataaacagtg cgccaaacat
    84961 aatgcctact gtcatcaggg tgaaaacaat actgtagcgt agctttccgt ccatcaatga
    85021 atgcagcgca atccccacca ctaccgcaac gggcatcagc gccagaaaga aaggccaggt
    85081 gtagataaag aagaacagcg tgttagagcc ataaatcaac atcggcatcg ccagcgcaaa
    85141 taaccaggag ataaaaccga ccacggcacc aggcagtgac catgtggttt cttcatcctc
    85201 agtaaggctg tcgttatttg ttagtgtaat gttatggcta ttacgcatat ttgatcctgt
    85261 tactttgacg aaccgggcat ggaaacccgg tggtgtctca ggatctgata atatcgttct
    85321 gtctcaacag atctaataat tgctgtacca aatttgttac taattgttca ccattgagat
    85381 gaatttctgc cgattcaggc gcttcgtaaa cggaatctat tcccgtaaag ttgcgcagtt
    85441 caccggcacg cgctttctta tataagcctt ttggatcgcg ggcttcgcaa atcgccagtg
    85501 gcgtatcgac aaacacttcg ataaagcgcc cttctcctac gcgttcgcga accatctggc
    85561 gttcggcgcg gtgtggcgag ataaatgcgg tcagcaccac cagtccggct tcaaccatca
    85621 aattcgccac ttcaccgacg cgacggatat tctctttacg atcggcatcg ctaaaaccga
    85681 gatcgctgca taatccgtgg cgaacattgt cgccatccag cagatacgta ctgacgccga
    85741 gtttatgtaa cgcctcctcc agcgccccgg cgaccgttga tttaccggac ccggagaggc
    85801 cggtaaacca cagcactaca ccacgatgac cgtggtgtag ctcgcgttgt tgcacagtga
    85861 ccggatggct atgccagacg acgttttcgt catgcagcgc cattatttct cccccagcaa
    85921 atcgcgcgcg ccccagtgtg gaaagtggcg gcgaaccagg gcattcaatt ccagttcgaa
    85981 tgcactaaat tcagatggcg cagcagttgc ctggctaact ggctcatgca ccataccggc
    86041 acctacggtc acattgctca ggcgatcgat aaaaatcagc ccaccggtaa ccgggttttg
    86101 ctgataacga tctaacacca gtggctcgtc aaaagtgaga tccaccaggc cgatgccgtt
    86161 cagcggcagg ttttcaactt cgcgttgggt aaggttatta atatcaacct gataacgaat
    86221 gccatcaaca cgagaacgcg tcttcttacc ggcaattttg atgtcgtaac tctggcccgg
    86281 ggaaagcggc tgttccgcca tccataccac atccaccgac gcgctctgca cagctggtaa
    86341 cgcttcgtct gccgccagca gcagatcgcc acggctgatg tcgatctcat ccgtcagcac
    86401 cagggtgata gcttctccgg caaaggcttc ttcgcgatca ccatcaaaag tcacgatccg
    86461 cgcgacgttt gattccacac cagagggcag cacttttaca cgttgcccga cttccacgcg
    86521 accggatgcc agcgttccgg cgtagccacg aaaatcgaga tttgggcggt taacgtactg
    86581 caccgggaag cgcattggct gggcatccac cactcgctga atctccacgg tttccagcac
    86641 ttcgagcagt gtcggaccgc tgtaccacgg catactttca ctttgcgaag ccacgttgtc
    86701 accttccagt gcggagagcg gcacaaagcg gatatccaga ttacccggca gctgcccggc
    86761 aaaggtcaga taatcttcac gaatacgggt gaacgtctct tcactgtaat ccaccagatc
    86821 cattttgttg atcgccacga ccagatgttt gatccccaac agtgtggaga taaaactgtg
    86881 acgacgggtt tgatcgagca cgcctttacg ggcatcgatc agtaagatcg ccagttcaca
    86941 tgtcgatgcg ccagtcgcca tattgcgggt gtactgctcg tgccctggag tgtcggcgat
    87001 aataaattta cgcttctcgg tagagaaata gcggtaggcc acgtcaatgg tgatgccctg
    87061 ttcacgctca gcttgcaggc cgtccaccag cagagccaga tccagctttt cgccctgggt
    87121 gccgtgacgc ttactgtcat tatgcagcga tgagagctga tcttcataga tttggcgggt
    87181 atcgtgcaac agacgaccaa tcagggtact tttgccgtca tcgacgctac cacaggtcag
    87241 aaaacgcagc aggcttttat gttgttgcgc aatcatccag gcttcgacgc cgccttcatt
    87301 ggcgatttgt tgtgcaagtg cggtgttcat cttaaaaata cccctgacgt tttttcagct
    87361 ccatagaacc agcttggtcg cggtcaatca cgcgcccctg acgttcactg gtggtggaaa
    87421 ccagcatctc ttcgatgatc tccggcagcg tttgtgcatt tgactccacc gcaccggtca
    87481 gcggccagca gcccagcgta cggaaacgca ccatccgttt tttaatcact tcgcccggtt
    87541 gcaggtcgat acggttgtca tcaatcatca tcaacatacc gtcgcgttcc agaaccggac
    87601 gttccgcagc gagatacagc ggcacaatgt cgatattttc cagccagatg tattgccaga
    87661 tatcctgctc ggtccagtta gagagcggga aaacgcggat gctttcgcct ttgttaatct
    87721 gcccgttata gttgtgccac agctccgggc gctgattttt cgggtcccag cgatggaagc
    87781 gatcacggaa agagtagata cgctctttag cacgggattt ctcttcgtcg cggcgcgcac
    87841 caccgaaggc ggcatcaaaa ccgtatttat tcagcgcctg cttcaggcct tcggtcttca
    87901 taatatcggt atgtttcgcg ctgccgtgca cgaatggatt aatccccatc gccacgcctt
    87961 ccgggttttg atgcaccagc agctcgcagc cgtaggcttt cgccgtacga tcgcggaact
    88021 catacatctc acggaatttc cagccggtat cgacatgcag caacgggaaa ggcagcgtac
    88081 ctggataaaa cgccttgcgc gccagatgca gcatgacgct ggaatcttta ccgatagagt
    88141 agagcatcac cggatttgag aattctgccg ccacctcgcg aataatgtgg atgctttccg
    88201 cctccagttg ccgcaggtga gtaagtcgta tttgatccat aaccgttcct ttgcaatacc
    88261 gctattttct tgccatcaga tgtttcgact atagggagcg taagagaacg aatgaaatta
    88321 ccaattagaa tgagtagttc cttaacggaa taacgatttg gcaaagctaa tatcaaaaag
    88381 tgcttaaggc accggatttc gggcgtttag gaagatttga aattgtttta gcgcagcggc
    88441 agtttcatac tatggcggta aaaaaatttg catggtattt aaggactcac tatgttttcc
    88501 gcattgcgcc accgtaccgc tgccctggcg ctcggcgtat gctttattct ccccgtacac
    88561 gcctcgtcac ctaaacctgg cgattttgcc aatacacagg cgcgacatat tgccactttc
    88621 tttccgggac gaatgaccgg aacacccgca gaaatgttat ctgccgatta tattcggcaa
    88681 cagtttcagc aaatgggtta ccgcagtgat attcgtacgt ttaatagccg atatatttat
    88741 accgcccgcg ataaccgcaa aaactggcac aacgtgacgg gaagtacggt gattgccgct
    88801 catgaaggca aagcgccgca gcagatcatt attatggcgc atctggatac ctatgccccg
    88861 cagagcgacg cagatgcaga tgccaatctc ggcgggctga cgttacaagg aatggatgat
    88921 aacgccgcag gtttaggtgt catgctggaa ctggcagaac gcctgaaaaa tacgcctacc
    88981 gagtatggta ttcgatttgt ggcgaccagt ggagaagagg aagggaaatt aggcgctgag
    89041 aatttactca agcggatgag tgacaccgaa aagaaaaata cgctgctggt gattaatctc
    89101 gataacttaa ttgttggcga taaattgtat ttcaacagcg gtgtaaaaac ccctgaagca
    89161 gtaaggaaat taacgcgcga cagggcgctg gcaattgcgc gtagtcatgg aattgccgca
    89221 acgaccaatc cgggtttgaa taaaaattat ccgaaaggca ctggatgttg taatgacgca
    89281 gaaatattcg acaaagcggg cattgctgta ctttcggtgg aagcgacaaa ctggaatctt
    89341 gggaataaag atggttatca gcaacgcgca aaaacagccg cattccctgc gggaaatagc
    89401 tggcatgacg taagactgga taatcagcaa catattgata aagcacttcc tggaagaata
    89461 gaacgtcgct gccgtgacgt tatgcggata atgctaccgc tggtgaagga gttggcgaag
    89521 gcgtcttgat gggttggaaa atgggagctg ggtgttctac cgcaggggcg gggaattcta
    89581 agtgatatcc atcatcgcat ccagtgcgcc cggtttatcc ccgctgatgc ggggaacaca
    89641 gcggcacgct ggattgaaca aatccctggg ccggtttatc cccgctggcg cggggaacac
    89701 aaaccgaaac acacgatcaa tccgaatatg agcggtttat ccccgctggc gcggggaaca
    89761 ctttggtgac agtttttgtc actgttttgg tgacggttta tccccgctgg cgcggggaac
    89821 acactaagca tacatatctg tttttaaaca aatttattcc acatcaacaa tctaccaact
    89881 aaattcaaac atttccttat ttttaaagaa cacataacct attgattatc aacaggaaga
    89941 aaagaaacca aacgtaaccc atccaaatcc accggaatac gtctgttttc tccccaggtc
    90001 tgaaattcaa aacccgactc ggtattggtc gcccaggcca tcaccacatt tccgcaacca
    90061 gccagttggg taatttgctg ccagatcatc tcccgaatac gttttgatgt atcaccaaca
    90121 tacacaccgg cacgcacttc cagtagccag attgcgagcc gtccacgtaa gcgcggaggg
    90181 acattttctg taacaaccac gaccatgctc atccgccgcg cccccggtga ccactatcac
    90241 ccagcgtttc aggttcaggg atggcaggcg gtaacatatc cggcgcgggt tgtggtggtt
    90301 caatttcacc tgcagcaagg actttctcaa ttaacggtat taatttgccc gttaacttag
    90361 tgctacggaa aatatcgcga caggctaatc tgacttcttt atcaggttct gcgggttgcc
    90421 tcgctgctat ttcaaatgcc tttggcacaa ccgaatcaaa tttaatgata tcggctatgt
    90481 cataaacaaa tgaaagcggt ttgccactat gaataaatcc aatagcgggc gcatatcccg
    90541 cggctaatac tgccgcttca gaaataccgt acagacatga tgtggcagca ctgatgcagc
    90601 gattcacaac atcgcctttt tcccagtctt taggatcgta tttgcgacca ttccatttca
    90661 caccatattg tttcgccagt aatgcatagg tctggcgaac gcgggatccc tcaattcccc
    90721 gtagctgatc cactgaacgg cgagctggcg gtggctcacg aaaacgtaat tcatacattt
    90781 tgcgcaccac cttcaggcgt agatcttccg ttaaagccag ctttgcctgg tagagtaatt
    90841 tatctgcccg cgcccctccg ggttgtccgg aagagtaaac gcgaacgccc gcttcaccga
    90901 cccagaccag cagtgttccc accgtggcgg ccagatgcac cgccgcgtgg gaaactctcg
    90961 ttcccggttc gagcataatg caggcgaccg atcccaccgg aatgtgcgtg cggatcccgg
    91021 ttttgtcgat cagcacgaaa gcgccgtcca gtacgtcgat ttgaccgtac tggaggaaga
    91081 tcatagaggt gcgatctttt aacgggatcg gactcagtgg tacaaacgtc acacctctgc
    91141 tccgggtttg atcagcatca gaccacagcc gaacgcgcgg cttttgccat acccctgact
    91201 gagacgttgc agaaataacc cgggatcggt gaccgtaagc atccccgtat aatccacgct
    91261 actgaactgg atcagttgcc gggagttttc ccgccgcagt tgctgttgtc ggtaggcatc
    91321 aaccgaggta tccagcagtg taaaaccgct tctctccccc tgcgctgcca gccagtccag
    91381 cgccgcctgt tgttgatgca accagacatc acttccttcc gcctgccccc tcacctgccg
    91441 tttcgcctcc atcagcagat cgtggcgctt gcccgcttta cagattgttg gattcgcccg
    91501 caggttgaaa caaagttgtt gtccggtacg cagctcgggc acaaatgacc ggcattcgat
    91561 ggtgaacgtt tcactttccg ccgggcgttc ctgcgacagt acaaaaaagc gaaacgcgcc
    91621 ctggagttct tcccgacgat aaagaaattg cctttctttg ccgccaggga agagatccca
    91681 cagccactga tgcatcacat attccccgcg atccaccaaa tgcagcaact gcgcaggcga
    91741 aagctggccg gtatgtaagg ttattcttga gaggtacatg gttcctcctt gctgagccac
    91801 ggcccctgat tgatggtgcg ttccccaaac agccactgct gacgatttaa aggaacatcc
    91861 cggcgacgta atattttgct cgcgaccagg ccgtcgtgtt ccccttccca ccagcattca
    91921 tcctgaagtt tcgggagtga gactttcagt tcgcggaaac tatcctgata ctgttggtat
    91981 gcgttacgta agacatcaga cgcgttgcct tcgagcagta acggcgcaag tggtaacgcc
    92041 agcggatgac tttttcgccc cagataaagc ggaaaaaccg gatgacgtaa accgtcctgc
    92101 aactgttcaa ggctgtaagg cgcatcgggg gttgttgcca ccgccaccat ccaccaggca
    92161 tcggtgtagt agtcgcgccg ggagataatc gcgctcagaa gatcaggggc gctcaactct
    92221 tcgcgacggc tgaaataacg cgctttacgc acctcttttg gcatctggac cgtgtgataa
    92281 tcccgtgccc agcgcgggtt acggctggcg caaaccacca gtgaatagtg gcggttaaac
    92341 gcgtttaatc gttcggtatc atcacgccga atccctaccc cggcagccag cagccccagc
    92401 aatgctgagc gcgaaggcag ttcatgggta tgacgcactt cgccgggggc atcgacgccc
    92461 caggatgcca ttggcccatg aagctgaaaa atcaaatatt ggctcattag ccgcccctta
    92521 cgcgcagata aagtccagca cgtccttcat gcttccctgt ttgttcatca cgtcaaagct
    92581 tgcgcattcg gtcttctgtt catagaccgt attcatattt tcgcgaagcg ttgtaatacg
    92641 ctgcaccgcc acatctaact gccgggtgcc attaatgggt tcatagaaag ccgccgccag
    92701 agaacgtggt tgttcggtgc ctttttctgc cagcgcccag gaggcgtagg cacggctggc
    92761 aaagctgttc tgtttgccgg ttggggagac tttaagtgcg gcttccgtaa aggcgcgcaa
    92821 ggtctgatta gctaacgctt cgtcaccgcc gaggttttcg accagcagat ctttatcgat
    92881 gcagatatag gtgtagaaca gcgcagaacc gaatccggtt tcaccaagat gcccggcacc
    92941 ggcatcttca gaagcctggc gcaaatcatc aacggcggtg aaaaaatcat cttcgacaat
    93001 cgtttcactg acaccaaatg catgcgcgac ctggcaggcg gcttcaacat taaattcggg
    93061 tttattcgcc agcatacgac caaacatagc gatatctact gccatgcgat ctttacgtaa
    93121 caaagcgaga tcttcctctt ttggcgcgcg cttttcttcg gccagttgat gggccagcgc
    93181 ttttacggcg tcaaattctg ccgggctgat atggactaat tgttcagttt cggcgttagt
    93241 gagcggatct tttggttttt tgtcgttttt agctttccca agataatccg caatttttgc
    93301 cgcccattcg atggcttttt tctcttcgat gcctttctca atcaggatag ttgccgcctc
    93361 acgcgcaata cgcccactgc gaataccaat atggcccgcc agtgcctgtt caaaaagtgc
    93421 agaagtgcgc cacgcacgtt tcagactttg cgaggaaacg cgcagtcgcg ttgctccacc
    93481 caggaccacg gttttcggcg ctccggtgtc atcacggtta aggttggctg cagggtaagc
    93541 ggttaacaaa tgaagctgaa taaatgtcgt catgagatag tcctttattg attttgttcg
    93601 ttatcgacgt cgccagcctg gtaatattcc agcgcccagc ggatacggat aaattcggtt
    93661 ggccgctgat gacggcggtg atgattgagc agatcgtcgc tctcctggca ccagcggaag
    93721 acaccctctg ccagagagtc aagattaacg gaaccgttta ataatctgac tacgcggcgt
    93781 aactggcgca gtaattcatc cggtgttttt actgccgaca ggcgagtaaa acgccccttt
    93841 gacattaccg ccgccagctg cgcagcaaaa ggcagccgtt catcaatggc tttaacatta
    93901 gcgctaagtg cggctatcag cgccagtgcg gtaatacgcc attcaggttc atcctgccac
    93961 tttatttgtc tgttcttcag aaacaggcga aatccatccg tcagacaaac atcattaacc
    94021 gtcgtactac gccgcagact ggcacgttcg ccgcgtttct cctgcaattc ctcatgccat
    94081 ttgcgcagcg tggctttgtg ctcctctttt acaatactca ttcagcagcc tcctgctttt
    94141 tctccctggc ggctttagca ctttgcttct ccgccgatgt tgtaaaatat ttcttgcgcg
    94201 cggtcatgac gcgttccaaa tcaacgggct cataaggatt ggtgaataca cgctcgtcaa
    94261 aatcctgacg tgcgaataac caaatttcct tttgccattt gccgagtaat tcatccgcat
    94321 cctgaccttc ttcaatttgg cgcactaacc tcaggaagcg atgctgagtt ttgttccaga
    94381 agtcgatatc cacaaaactg aaatcacccc ttgcaccttt tggatcggag aaccatgctt
    94441 ctttcaatgc actccgtaac agactcagaa tccgtgaagc cgtttgcgca gccagccgca
    94501 gcttcggtat ctggccttct tttttattga gcagcagcgg gaaatggtgt tcgtaccaac
    94561 agcgcgcttt catgttgtcg aaatcataac caaatcccca caggcccact tttgcctgtt
    94621 tcagactgct ggcattaaag agtttcacca ccagcgcggg aagttccgta ttgttttctg
    94681 acttacccgt ttcgataagg cctaaccagt cgcgccagat taaaccgccc ggttgtggtt
    94741 taacggagta aaactcaccg ccctctttaa gtggtacacg gtaaggcgtt aagggatgct
    94801 gccacatggc ataattcgca ccgtaatttt tggtagtcat caaactcaga agcgcgtcac
    94861 tctgctcacc gcaaatatcg cagttgccga ctgtcgtggt attaaaatca atacgaatac
    94921 gccgcggcat tccccagtac gcctggagtt tattgacctg atcatcggtt accaccgcac
    94981 cggccagttc gctggtacgc gtcgggccaa gccaggggaa aaccagatcg tcaaattttt
    95041 tgggtagcgg taagtcggct tcatcctgcg gcatcacgtt gagccacagt ttgcgccaca
    95101 agggggcttg ttgattgccc tgatactcct gcaattcaat cagagtcgtc atcggcccac
    95161 cgccgcgtaa accggtgcga tagcctttgc cacctgacgg cgcatttaac tgtagggaga
    95221 acagagctaa cgcagaacaa tgagagcata cgtgttcagt cacgccacgc ttaataaagt
    95281 ggtctttatt aaacttcgtt gtttgagcgc cgggaatctc aggcagtagc gaagcgacct
    95341 gaactttatc gcccatgagc acctcgaaat cctgcataaa tgaaggtgaa tctgggccaa
    95401 actggaaagg gtgttctaat gacagcaatg cttcccgtag cttttcagct tccagcccgt
    95461 cttcccagat atcatcccaa cgacgataat cttttggcgc gaaactgctt tgtagtaacc
    95521 ccagcaaaaa ctgccatgcc gccccctgga gatctgcccg cggcgcagcg atatcgacaa
    95581 cattttcatc cgccagatcg actggcgcca gcttgcctgt tgttccgtct ttaaaacgaa
    95641 cgggcaacca cggggttgtc agaagtgaaa acgagttcat atgtatcctc tcgtaaaaaa
    95701 gaccatccct ggtcggttgt ccatcattcc atccttttcg gtgtatcaac tattcagaga
    95761 gtcctgtcgc aggtatctca atcaaccttg ccaatcaatc cctccctggc cgaatacccg
    95821 caactttcgt catcagtgac taaaatcacg tttgccattt ccggatcttg ccgctgttca
    95881 atgcaccact gcctgaacgc ttccccttcc agtaaagaaa actcatcccg atgttttttc
    95941 caccagcttc gacgcactct gacaacgctc atttcccatg cgtgagcacc ggtggcataa
    96001 ggcttcacca caccggcaat acaggtagcc agccacaggg aaacagattc ctcagccaga
    96061 cgtgtcgaca gcttttccgg aaggtaatcg ttgatattgg cggcatagcc aggcttgaag
    96121 ttcaggacaa actttttagc cattgcgcga tcgcagtaat atttgcccac ttgctcctgc
    96181 tcgctgcggg caaatccttc cggcattacc acgtcctcac cgtagactga ttcaataaga
    96241 aggcgggctg cgtgtggcat ttgaatagcg ccttgctcac gcagtacacg ctgcgtcagc
    96301 cagattcgtc catgatcggg atagacatat gcactgttac gcatggcact gccgaaccat
    96361 tcgtcaccag gagcgtcgtc ccagacgggg gccagaatca gcaattcagg aggggaacgc
    96421 tcgtcttttc cgtcacgctt taactgacca ttaatatcgc ggatatgccg ctgtaatcgc
    96481 cccgctcgct gaatcagcaa atcaacaggg gccaggtcgg agatcatttc gtccaggtca
    96541 caatcaacgc tctgctctaa gacctgagta caaatgagga cttttccggc acgctgtgaa
    96601 ccgtcttctt taccaaagcg tgccagcgtc tccatttcaa ttcgctggcg atcgctaaaa
    96661 gcaaagcggc tatgaaagag tgaaaggctg gaagcgggaa tgacgccgcg ggcaagcagc
    96721 tgacgatgaa ccttaatagc gtcatcgaca gaattccgga tccaggcgat gcattttccc
    96781 tgacttaccg ccgattcgat acgcgcaata ctctcttgtt cactatgaag ccaacccacg
    96841 ctgacgctac gctcaacgtc tttgcgcgtc gctacccggt gtgagttcac atcggatttc
    96901 gtgacatgcg tcagccaggg gtaatcatcc ttttcaagga acggagcttc ttgctggccc
    96961 tctgtgccac gcgcaaaggc ggcgacgagt ttgtcgcgct gctgttggga taacgtagca
    97021 gaaagcaaaa tgacgctgtt tccgccacgc gcctgccgct cgatcagccc ttcaagaatg
    97081 cacgacatgt aagcatcaca ggcatggatc tcatcagcca gcaggatttt gttactcaac
    97141 cccagaagcc gcagattatt atgtttaaac ggcatcactg ccatcatcgc ctgatccagc
    97201 gtgccgacgc caatttcagc cagtagcgcc ttcttgttac tgttggcaaa ccaggccgca
    97261 catccctgac tgaatgtttg ttcatccggt tcttctgacc cgactaaatc accggaccag
    97321 agtgattcat tgaagcggtc cattaatgtg cgggcactgt gtgccagcac caagctgggg
    97381 cgggactctg gcgaatagaa agcaagccag gttttgacca gccgatcgta catggcattg
    97441 gccgttgcca ttgttggcag gccaaaaaac aaaccctgtg ctttcctcgc agccatcaac
    97501 ctgtgcgcca ggataagcgc cgcttctgtt ttacctgcgc cagtcacgtc ttccagaata
    97561 aataactgtg gccctggctg gctgatatcc agatccagta ccttttgctg taatggtgtc
    97621 gggtgctcaa taaaaggaaa cagcgtatta attccggtga aaggtgcggt ttctgctttt
    97681 ggaggaaaga cggttaaggc gttttgagcc tgaactaaag ttttctgcca gtaatcttta
    97741 atatccattg ggtgtgcgac gcgtggaaaa aatcgcgttg acgaacccgt ccagtctgcg
    97801 agtacgactg ttgcagagat ataccaggaa agttgtttta aaagttcaac gccctcgtca
    97861 tcatcccaga atgtgggaat ctctatgagc ggaaacagtg ccttgatttc aaggagaaaa
    97921 tctcgcgcgg cagctttgtc ttcaggcaga aaattatcca gctcatcaat acggtcaggt
    97981 ggtcgaccat gatgcccggt agttatggac atccacatct ctattacacg tgtaagttta
    98041 cgagaagaga gtgaagatga aggaagcaac tcctcacatt cacttaaata ataattccac
    98101 agccagtaac ccagcgttga atgagagatc ttttcgtaat tctttctgga accttccgga
    98161 atcttgagtt caggggccag gtaaagttgc tgaaaagagc gggcaaattt tccaatatcg
    98221 tgccagcaca gcaaccaagc gaaaaattga gccgcctgtt ccttgtcaga aatccctaat
    98281 tgacgaaagt aatcagccag cccgaagcaa tttcttttaa ccattaaata gcccattgcg
    98341 gccacatcca gcgaatgcca gcaaagaagg tgatagccgt cgccaccctc tttctcgcca
    98401 cgtcgggttt ttccccagaa atcaaagaaa gtcacaatat ttttatcctt cagtaaactt
    98461 aaaggatatt tacgcatatt ttaaaaatta tct
//
LOCUS       DABSYT020000008        90292 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.008, whole genome shotgun sequence.
ACCESSION   DABSYT020000008 DABSYT020000000
VERSION     DABSYT020000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 90292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.008"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            56..640
                     /gene="yraH"
                     /locus_tag="ID725_RS03910"
                     /old_locus_tag="pgaptmp_000782"
     CDS             56..640
                     /gene="yraH"
                     /locus_tag="ID725_RS03910"
                     /old_locus_tag="pgaptmp_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7849447.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            720..1415
                     /gene="yraI"
                     /locus_tag="ID725_RS03915"
                     /old_locus_tag="pgaptmp_000783"
     CDS             720..1415
                     /gene="yraI"
                     /locus_tag="ID725_RS03915"
                     /old_locus_tag="pgaptmp_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGV7849448.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1444..3960
                     /gene="yraJ"
                     /locus_tag="ID725_RS03920"
                     /old_locus_tag="pgaptmp_000784"
     CDS             1444..3960
                     /gene="yraJ"
                     /locus_tag="ID725_RS03920"
                     /old_locus_tag="pgaptmp_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGV7849449.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3971..4704
                     /locus_tag="ID725_RS03925"
                     /old_locus_tag="pgaptmp_000785"
                     /pseudo
     CDS             3971..4704
                     /locus_tag="ID725_RS03925"
                     /old_locus_tag="pgaptmp_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(4747..5607)
                     /gene="rsmI"
                     /locus_tag="ID725_RS03930"
                     /old_locus_tag="pgaptmp_000786"
     CDS             complement(4747..5607)
                     /gene="rsmI"
                     /locus_tag="ID725_RS03930"
                     /old_locus_tag="pgaptmp_000786"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGV7849450.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5672..7708
                     /gene="lpoA"
                     /locus_tag="ID725_RS03935"
                     /old_locus_tag="pgaptmp_000787"
     CDS             5672..7708
                     /gene="lpoA"
                     /locus_tag="ID725_RS03935"
                     /old_locus_tag="pgaptmp_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGV7849451.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7666..8061
                     /gene="yraN"
                     /locus_tag="ID725_RS03940"
                     /old_locus_tag="pgaptmp_000788"
     CDS             7666..8061
                     /gene="yraN"
                     /locus_tag="ID725_RS03940"
                     /old_locus_tag="pgaptmp_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGV7849452.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            8081..8671
                     /gene="diaA"
                     /locus_tag="ID725_RS03945"
                     /old_locus_tag="pgaptmp_000789"
     CDS             8081..8671
                     /gene="diaA"
                     /locus_tag="ID725_RS03945"
                     /old_locus_tag="pgaptmp_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGV7849453.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8681..9256
                     /gene="dolP"
                     /locus_tag="ID725_RS03950"
                     /old_locus_tag="pgaptmp_000790"
     CDS             8681..9256
                     /gene="dolP"
                     /locus_tag="ID725_RS03950"
                     /old_locus_tag="pgaptmp_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGV7849454.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9370..10410)
                     /gene="yraQ"
                     /locus_tag="ID725_RS03955"
                     /old_locus_tag="pgaptmp_000791"
     CDS             complement(9370..10410)
                     /gene="yraQ"
                     /locus_tag="ID725_RS03955"
                     /old_locus_tag="pgaptmp_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGV7849455.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10483..11118)
                     /gene="yraR"
                     /locus_tag="ID725_RS03960"
                     /old_locus_tag="pgaptmp_000792"
     CDS             complement(10483..11118)
                     /gene="yraR"
                     /locus_tag="ID725_RS03960"
                     /old_locus_tag="pgaptmp_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGV7849456.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11246..11764
                     /gene="yhbO"
                     /locus_tag="ID725_RS03965"
                     /old_locus_tag="pgaptmp_000793"
     CDS             11246..11764
                     /gene="yhbO"
                     /locus_tag="ID725_RS03965"
                     /old_locus_tag="pgaptmp_000793"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGV7849457.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(11744..12187)
                     /gene="yhbP"
                     /locus_tag="ID725_RS03970"
                     /old_locus_tag="pgaptmp_000794"
     CDS             complement(11744..12187)
                     /gene="yhbP"
                     /locus_tag="ID725_RS03970"
                     /old_locus_tag="pgaptmp_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGV7849458.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMRGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12238..12540
                     /gene="yhbQ"
                     /locus_tag="ID725_RS03975"
                     /old_locus_tag="pgaptmp_000795"
     CDS             12238..12540
                     /gene="yhbQ"
                     /locus_tag="ID725_RS03975"
                     /old_locus_tag="pgaptmp_000795"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGV7849459.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(12527..13030)
                     /gene="yhbS"
                     /locus_tag="ID725_RS03980"
                     /old_locus_tag="pgaptmp_000796"
     CDS             complement(12527..13030)
                     /gene="yhbS"
                     /locus_tag="ID725_RS03980"
                     /old_locus_tag="pgaptmp_000796"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGV7849460.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13024..13548)
                     /gene="ubiT"
                     /locus_tag="ID725_RS03985"
                     /old_locus_tag="pgaptmp_000797"
     CDS             complement(13024..13548)
                     /gene="ubiT"
                     /locus_tag="ID725_RS03985"
                     /old_locus_tag="pgaptmp_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGV7849461.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            13757..14752
                     /gene="ubiU"
                     /locus_tag="ID725_RS03990"
                     /old_locus_tag="pgaptmp_000798"
     CDS             13757..14752
                     /gene="ubiU"
                     /locus_tag="ID725_RS03990"
                     /old_locus_tag="pgaptmp_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGV7849462.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            14761..15639
                     /gene="ubiV"
                     /locus_tag="ID725_RS03995"
                     /old_locus_tag="pgaptmp_000799"
     CDS             14761..15639
                     /gene="ubiV"
                     /locus_tag="ID725_RS03995"
                     /old_locus_tag="pgaptmp_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGV7849463.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            15720..16727
                     /gene="yhbW"
                     /locus_tag="ID725_RS04000"
                     /old_locus_tag="pgaptmp_000800"
     CDS             15720..16727
                     /gene="yhbW"
                     /locus_tag="ID725_RS04000"
                     /old_locus_tag="pgaptmp_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGV7849464.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(16844..18088)
                     /gene="mtr"
                     /locus_tag="ID725_RS04005"
                     /old_locus_tag="pgaptmp_000801"
     CDS             complement(16844..18088)
                     /gene="mtr"
                     /locus_tag="ID725_RS04005"
                     /old_locus_tag="pgaptmp_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGV7849465.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18242..20131)
                     /gene="deaD"
                     /locus_tag="ID725_RS04010"
                     /old_locus_tag="pgaptmp_000802"
     CDS             complement(18242..20131)
                     /gene="deaD"
                     /locus_tag="ID725_RS04010"
                     /old_locus_tag="pgaptmp_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGV7849466.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20124..20204)
                     /gene="yrbN"
                     /locus_tag="ID725_RS04015"
                     /old_locus_tag="pgaptmp_000803"
     CDS             complement(20124..20204)
                     /gene="yrbN"
                     /locus_tag="ID725_RS04015"
                     /old_locus_tag="pgaptmp_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGV7849467.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20311..21195)
                     /gene="nlpI"
                     /locus_tag="ID725_RS04020"
                     /old_locus_tag="pgaptmp_000804"
     CDS             complement(20311..21195)
                     /gene="nlpI"
                     /locus_tag="ID725_RS04020"
                     /old_locus_tag="pgaptmp_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGV7849468.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21304..23439)
                     /gene="pnp"
                     /locus_tag="ID725_RS04025"
                     /old_locus_tag="pgaptmp_000805"
     CDS             complement(21304..23439)
                     /gene="pnp"
                     /locus_tag="ID725_RS04025"
                     /old_locus_tag="pgaptmp_000805"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGV7849469.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23686..23955)
                     /gene="rpsO"
                     /locus_tag="ID725_RS04030"
                     /old_locus_tag="pgaptmp_000806"
     CDS             complement(23686..23955)
                     /gene="rpsO"
                     /locus_tag="ID725_RS04030"
                     /old_locus_tag="pgaptmp_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGV7849470.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(24104..25048)
                     /gene="truB"
                     /locus_tag="ID725_RS04035"
                     /old_locus_tag="pgaptmp_000807"
     CDS             complement(24104..25048)
                     /gene="truB"
                     /locus_tag="ID725_RS04035"
                     /old_locus_tag="pgaptmp_000807"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGV7849471.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDVDGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25048..25449)
                     /gene="rbfA"
                     /locus_tag="ID725_RS04040"
                     /old_locus_tag="pgaptmp_000808"
     CDS             complement(25048..25449)
                     /gene="rbfA"
                     /locus_tag="ID725_RS04040"
                     /old_locus_tag="pgaptmp_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGV7849472.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25614..28286)
                     /gene="infB"
                     /locus_tag="ID725_RS04045"
                     /old_locus_tag="pgaptmp_000809"
     CDS             complement(25614..28286)
                     /gene="infB"
                     /locus_tag="ID725_RS04045"
                     /old_locus_tag="pgaptmp_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGV7849473.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28311..29798)
                     /gene="nusA"
                     /locus_tag="ID725_RS04050"
                     /old_locus_tag="pgaptmp_000810"
     CDS             complement(28311..29798)
                     /gene="nusA"
                     /locus_tag="ID725_RS04050"
                     /old_locus_tag="pgaptmp_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGV7849474.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQVQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(29826..30278)
                     /gene="rimP"
                     /locus_tag="ID725_RS04055"
                     /old_locus_tag="pgaptmp_000811"
     CDS             complement(29826..30278)
                     /gene="rimP"
                     /locus_tag="ID725_RS04055"
                     /old_locus_tag="pgaptmp_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGV7849475.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30485..30561)
                     /locus_tag="ID725_RS04060"
                     /old_locus_tag="pgaptmp_000812"
     tRNA            complement(30485..30561)
                     /locus_tag="ID725_RS04060"
                     /old_locus_tag="pgaptmp_000812"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30525..30527),aa:Met,seq:cat)
     gene            30909..32252
                     /gene="argG"
                     /locus_tag="ID725_RS04065"
                     /old_locus_tag="pgaptmp_000813"
     CDS             30909..32252
                     /gene="argG"
                     /locus_tag="ID725_RS04065"
                     /old_locus_tag="pgaptmp_000813"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGV7849476.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(32260..33885)
                     /gene="yhbX"
                     /locus_tag="ID725_RS04070"
                     /old_locus_tag="pgaptmp_000814"
     CDS             complement(32260..33885)
                     /gene="yhbX"
                     /locus_tag="ID725_RS04070"
                     /old_locus_tag="pgaptmp_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGV7849477.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQSQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34344..34430)
                     /locus_tag="ID725_RS04075"
                     /old_locus_tag="pgaptmp_000815"
     tRNA            complement(34344..34430)
                     /locus_tag="ID725_RS04075"
                     /old_locus_tag="pgaptmp_000815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34394..34396),aa:Leu,seq:gag)
     gene            complement(34445..34777)
                     /gene="secG"
                     /locus_tag="ID725_RS04080"
                     /old_locus_tag="pgaptmp_000816"
     CDS             complement(34445..34777)
                     /gene="secG"
                     /locus_tag="ID725_RS04080"
                     /old_locus_tag="pgaptmp_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGV7849478.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35005..36342)
                     /gene="glmM"
                     /locus_tag="ID725_RS04085"
                     /old_locus_tag="pgaptmp_000817"
     CDS             complement(35005..36342)
                     /gene="glmM"
                     /locus_tag="ID725_RS04085"
                     /old_locus_tag="pgaptmp_000817"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGV7849479.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36335..37183)
                     /gene="folP"
                     /locus_tag="ID725_RS04090"
                     /old_locus_tag="pgaptmp_000818"
     CDS             complement(36335..37183)
                     /gene="folP"
                     /locus_tag="ID725_RS04090"
                     /old_locus_tag="pgaptmp_000818"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGV7849480.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37273..39207)
                     /gene="ftsH"
                     /locus_tag="ID725_RS04095"
                     /old_locus_tag="pgaptmp_000819"
     CDS             complement(37273..39207)
                     /gene="ftsH"
                     /locus_tag="ID725_RS04095"
                     /old_locus_tag="pgaptmp_000819"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGV7849481.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39307..39936)
                     /gene="rlmE"
                     /locus_tag="ID725_RS04100"
                     /old_locus_tag="pgaptmp_000820"
     CDS             complement(39307..39936)
                     /gene="rlmE"
                     /locus_tag="ID725_RS04100"
                     /old_locus_tag="pgaptmp_000820"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGV7849482.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40062..40355
                     /gene="yhbY"
                     /locus_tag="ID725_RS04105"
                     /old_locus_tag="pgaptmp_000821"
     CDS             40062..40355
                     /gene="yhbY"
                     /locus_tag="ID725_RS04105"
                     /old_locus_tag="pgaptmp_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGV7849483.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40511..40987)
                     /gene="greA"
                     /locus_tag="ID725_RS04110"
                     /old_locus_tag="pgaptmp_000822"
     CDS             complement(40511..40987)
                     /gene="greA"
                     /locus_tag="ID725_RS04110"
                     /old_locus_tag="pgaptmp_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGV7849484.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41235..42668
                     /gene="dacB"
                     /locus_tag="ID725_RS04115"
                     /old_locus_tag="pgaptmp_000823"
     CDS             41235..42668
                     /gene="dacB"
                     /locus_tag="ID725_RS04115"
                     /old_locus_tag="pgaptmp_000823"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGV7849485.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(42708..43880)
                     /gene="cgtA"
                     /locus_tag="ID725_RS04120"
                     /old_locus_tag="pgaptmp_000824"
     CDS             complement(42708..43880)
                     /gene="cgtA"
                     /locus_tag="ID725_RS04120"
                     /old_locus_tag="pgaptmp_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGV7849486.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(43896..44861)
                     /gene="yhbE"
                     /locus_tag="ID725_RS04125"
                     /old_locus_tag="pgaptmp_000825"
     CDS             complement(43896..44861)
                     /gene="yhbE"
                     /locus_tag="ID725_RS04125"
                     /old_locus_tag="pgaptmp_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGV7849487.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLKSLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(44988..45245)
                     /gene="rpmA"
                     /locus_tag="ID725_RS04130"
                     /old_locus_tag="pgaptmp_000826"
     CDS             complement(44988..45245)
                     /gene="rpmA"
                     /locus_tag="ID725_RS04130"
                     /old_locus_tag="pgaptmp_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGV7849488.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45266..45577)
                     /gene="rplU"
                     /locus_tag="ID725_RS04135"
                     /old_locus_tag="pgaptmp_000827"
     CDS             complement(45266..45577)
                     /gene="rplU"
                     /locus_tag="ID725_RS04135"
                     /old_locus_tag="pgaptmp_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGV7849489.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            45836..46807
                     /gene="ispB"
                     /locus_tag="ID725_RS04140"
                     /old_locus_tag="pgaptmp_000828"
     CDS             45836..46807
                     /gene="ispB"
                     /locus_tag="ID725_RS04140"
                     /old_locus_tag="pgaptmp_000828"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGV7849490.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47036..47314
                     /gene="sfsB"
                     /locus_tag="ID725_RS04145"
                     /old_locus_tag="pgaptmp_000829"
     CDS             47036..47314
                     /gene="sfsB"
                     /locus_tag="ID725_RS04145"
                     /old_locus_tag="pgaptmp_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGV7849491.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47362..48621)
                     /gene="murA"
                     /locus_tag="ID725_RS04150"
                     /old_locus_tag="pgaptmp_000830"
     CDS             complement(47362..48621)
                     /gene="murA"
                     /locus_tag="ID725_RS04150"
                     /old_locus_tag="pgaptmp_000830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGV7849492.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48676..48930)
                     /gene="ibaG"
                     /locus_tag="ID725_RS04155"
                     /old_locus_tag="pgaptmp_000831"
     CDS             complement(48676..48930)
                     /gene="ibaG"
                     /locus_tag="ID725_RS04155"
                     /old_locus_tag="pgaptmp_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGV7849493.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49090..49383)
                     /gene="mlaB"
                     /locus_tag="ID725_RS04160"
                     /old_locus_tag="pgaptmp_000832"
     CDS             complement(49090..49383)
                     /gene="mlaB"
                     /locus_tag="ID725_RS04160"
                     /old_locus_tag="pgaptmp_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGV7849494.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49383..50018)
                     /gene="mlaC"
                     /locus_tag="ID725_RS04165"
                     /old_locus_tag="pgaptmp_000833"
     CDS             complement(49383..50018)
                     /gene="mlaC"
                     /locus_tag="ID725_RS04165"
                     /old_locus_tag="pgaptmp_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGV7849495.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50037..50588)
                     /gene="mlaD"
                     /locus_tag="ID725_RS04170"
                     /old_locus_tag="pgaptmp_000834"
     CDS             complement(50037..50588)
                     /gene="mlaD"
                     /locus_tag="ID725_RS04170"
                     /old_locus_tag="pgaptmp_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGV7849496.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50593..51375)
                     /gene="mlaE"
                     /locus_tag="ID725_RS04175"
                     /old_locus_tag="pgaptmp_000835"
     CDS             complement(50593..51375)
                     /gene="mlaE"
                     /locus_tag="ID725_RS04175"
                     /old_locus_tag="pgaptmp_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGV7849497.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51383..52192)
                     /gene="mlaF"
                     /locus_tag="ID725_RS04180"
                     /old_locus_tag="pgaptmp_000836"
     CDS             complement(51383..52192)
                     /gene="mlaF"
                     /locus_tag="ID725_RS04180"
                     /old_locus_tag="pgaptmp_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGV7849498.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52402..53379
                     /gene="yrbG"
                     /locus_tag="ID725_RS04185"
                     /old_locus_tag="pgaptmp_000837"
     CDS             52402..53379
                     /gene="yrbG"
                     /locus_tag="ID725_RS04185"
                     /old_locus_tag="pgaptmp_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGV7849499.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53393..54379
                     /gene="kdsD"
                     /locus_tag="ID725_RS04190"
                     /old_locus_tag="pgaptmp_000838"
     CDS             53393..54379
                     /gene="kdsD"
                     /locus_tag="ID725_RS04190"
                     /old_locus_tag="pgaptmp_000838"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGV7849500.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54400..54966
                     /gene="kdsC"
                     /locus_tag="ID725_RS04195"
                     /old_locus_tag="pgaptmp_000839"
     CDS             54400..54966
                     /gene="kdsC"
                     /locus_tag="ID725_RS04195"
                     /old_locus_tag="pgaptmp_000839"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGV7849501.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            54963..55538
                     /gene="lptC"
                     /locus_tag="ID725_RS04200"
                     /old_locus_tag="pgaptmp_000840"
     CDS             54963..55538
                     /gene="lptC"
                     /locus_tag="ID725_RS04200"
                     /old_locus_tag="pgaptmp_000840"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGV7849502.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55507..56064
                     /gene="lptA"
                     /locus_tag="ID725_RS04205"
                     /old_locus_tag="pgaptmp_000841"
     CDS             55507..56064
                     /gene="lptA"
                     /locus_tag="ID725_RS04205"
                     /old_locus_tag="pgaptmp_000841"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGV7849503.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56071..56796
                     /gene="lptB"
                     /locus_tag="ID725_RS04210"
                     /old_locus_tag="pgaptmp_000842"
     CDS             56071..56796
                     /gene="lptB"
                     /locus_tag="ID725_RS04210"
                     /old_locus_tag="pgaptmp_000842"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGV7849504.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            56844..58277
                     /gene="rpoN"
                     /locus_tag="ID725_RS04215"
                     /old_locus_tag="pgaptmp_000843"
     CDS             56844..58277
                     /gene="rpoN"
                     /locus_tag="ID725_RS04215"
                     /old_locus_tag="pgaptmp_000843"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGV7849505.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58300..58587
                     /gene="hpf"
                     /locus_tag="ID725_RS04220"
                     /old_locus_tag="pgaptmp_000844"
     CDS             58300..58587
                     /gene="hpf"
                     /locus_tag="ID725_RS04220"
                     /old_locus_tag="pgaptmp_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGV7849506.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            58705..59196
                     /gene="ptsN"
                     /locus_tag="ID725_RS04225"
                     /old_locus_tag="pgaptmp_000845"
     CDS             58705..59196
                     /gene="ptsN"
                     /locus_tag="ID725_RS04225"
                     /old_locus_tag="pgaptmp_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGV7849507.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            59242..60096
                     /gene="rapZ"
                     /locus_tag="ID725_RS04230"
                     /old_locus_tag="pgaptmp_000846"
     CDS             59242..60096
                     /gene="rapZ"
                     /locus_tag="ID725_RS04230"
                     /old_locus_tag="pgaptmp_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGV7849508.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60093..60365
                     /gene="npr"
                     /locus_tag="ID725_RS04235"
                     /old_locus_tag="pgaptmp_000847"
     CDS             60093..60365
                     /gene="npr"
                     /locus_tag="ID725_RS04235"
                     /old_locus_tag="pgaptmp_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGV7849509.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60579..61211
                     /gene="yrbL"
                     /locus_tag="ID725_RS04240"
                     /old_locus_tag="pgaptmp_000848"
     CDS             60579..61211
                     /gene="yrbL"
                     /locus_tag="ID725_RS04240"
                     /old_locus_tag="pgaptmp_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGV7849510.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(61208..61936)
                     /gene="mtgA"
                     /locus_tag="ID725_RS04245"
                     /old_locus_tag="pgaptmp_000849"
     CDS             complement(61208..61936)
                     /gene="mtgA"
                     /locus_tag="ID725_RS04245"
                     /old_locus_tag="pgaptmp_000849"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGV7849511.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(61933..62586)
                     /gene="elbB"
                     /locus_tag="ID725_RS04250"
                     /old_locus_tag="pgaptmp_000850"
     CDS             complement(61933..62586)
                     /gene="elbB"
                     /locus_tag="ID725_RS04250"
                     /old_locus_tag="pgaptmp_000850"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGV7849512.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(62816..65152)
                     /gene="arcB"
                     /locus_tag="ID725_RS04255"
                     /old_locus_tag="pgaptmp_000851"
     CDS             complement(62816..65152)
                     /gene="arcB"
                     /locus_tag="ID725_RS04255"
                     /old_locus_tag="pgaptmp_000851"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGV7849513.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65248..66177)
                     /gene="yhcC"
                     /locus_tag="ID725_RS04260"
                     /old_locus_tag="pgaptmp_000852"
     CDS             complement(65248..66177)
                     /gene="yhcC"
                     /locus_tag="ID725_RS04260"
                     /old_locus_tag="pgaptmp_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGV7849514.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            66851..71311
                     /gene="gltB"
                     /locus_tag="ID725_RS04265"
                     /old_locus_tag="pgaptmp_000853"
     CDS             66851..71311
                     /gene="gltB"
                     /locus_tag="ID725_RS04265"
                     /old_locus_tag="pgaptmp_000853"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGV7849515.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71324..72742
                     /gene="gltD"
                     /locus_tag="ID725_RS04270"
                     /old_locus_tag="pgaptmp_000854"
     CDS             71324..72742
                     /gene="gltD"
                     /locus_tag="ID725_RS04270"
                     /old_locus_tag="pgaptmp_000854"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGV7849516.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            72926..73975
                     /gene="yhcG"
                     /locus_tag="ID725_RS04275"
                     /old_locus_tag="pgaptmp_000855"
     CDS             72926..73975
                     /gene="yhcG"
                     /locus_tag="ID725_RS04275"
                     /old_locus_tag="pgaptmp_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HGV7849517.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLPWSTYVRLLSVKNADTRSFYEKETLRCGWSVRQLERQIATQFYERTL
                     LSHDKSAMLQQHAPAETHILPQQAIRDPFVLEFLELKDEYSESDFEEALINHLMDFML
                     ELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLLIVDLKVGKFSYSDAGQMNMYL
                     NYAKEHWTLPDENPPIGLVLCAEKGVGEAHYALAGLPNTVLASEYKMQLPDEKRLADE
                     LVRTQAVLEEGYRLR"
     gene            complement(74035..74499)
                     /gene="nanQ"
                     /locus_tag="ID725_RS04280"
                     /old_locus_tag="pgaptmp_000856"
     CDS             complement(74035..74499)
                     /gene="nanQ"
                     /locus_tag="ID725_RS04280"
                     /old_locus_tag="pgaptmp_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGV7849518.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74496..75371)
                     /gene="nanK"
                     /locus_tag="ID725_RS04285"
                     /old_locus_tag="pgaptmp_000857"
     CDS             complement(74496..75371)
                     /gene="nanK"
                     /locus_tag="ID725_RS04285"
                     /old_locus_tag="pgaptmp_000857"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGV7849519.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75368..76057)
                     /gene="nanE"
                     /locus_tag="ID725_RS04290"
                     /old_locus_tag="pgaptmp_000858"
     CDS             complement(75368..76057)
                     /gene="nanE"
                     /locus_tag="ID725_RS04290"
                     /old_locus_tag="pgaptmp_000858"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGV7849520.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76105..77595)
                     /gene="nanT"
                     /locus_tag="ID725_RS04295"
                     /old_locus_tag="pgaptmp_000859"
     CDS             complement(76105..77595)
                     /gene="nanT"
                     /locus_tag="ID725_RS04295"
                     /old_locus_tag="pgaptmp_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGV7849521.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(77704..78597)
                     /gene="nanA"
                     /locus_tag="ID725_RS04300"
                     /old_locus_tag="pgaptmp_000860"
     CDS             complement(77704..78597)
                     /gene="nanA"
                     /locus_tag="ID725_RS04300"
                     /old_locus_tag="pgaptmp_000860"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGV7849522.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(78719..79510)
                     /gene="nanR"
                     /locus_tag="ID725_RS04305"
                     /old_locus_tag="pgaptmp_000861"
     CDS             complement(78719..79510)
                     /gene="nanR"
                     /locus_tag="ID725_RS04305"
                     /old_locus_tag="pgaptmp_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGV7849523.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            79890..81246
                     /gene="dcuC"
                     /locus_tag="ID725_RS04310"
                     /old_locus_tag="pgaptmp_000862"
                     /pseudo
     CDS             79890..81246
                     /gene="dcuC"
                     /locus_tag="ID725_RS04310"
                     /old_locus_tag="pgaptmp_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(81289..81786)
                     /gene="sspB"
                     /locus_tag="ID725_RS04315"
                     /old_locus_tag="pgaptmp_000863"
     CDS             complement(81289..81786)
                     /gene="sspB"
                     /locus_tag="ID725_RS04315"
                     /old_locus_tag="pgaptmp_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGV7849524.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(81792..82430)
                     /gene="sspA"
                     /locus_tag="ID725_RS04320"
                     /old_locus_tag="pgaptmp_000864"
     CDS             complement(81792..82430)
                     /gene="sspA"
                     /locus_tag="ID725_RS04320"
                     /old_locus_tag="pgaptmp_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGV7849525.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(82825..83217)
                     /gene="rpsI"
                     /locus_tag="ID725_RS04325"
                     /old_locus_tag="pgaptmp_000865"
     CDS             complement(82825..83217)
                     /gene="rpsI"
                     /locus_tag="ID725_RS04325"
                     /old_locus_tag="pgaptmp_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGV7849526.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(83233..83661)
                     /gene="rplM"
                     /locus_tag="ID725_RS04330"
                     /old_locus_tag="pgaptmp_000866"
     CDS             complement(83233..83661)
                     /gene="rplM"
                     /locus_tag="ID725_RS04330"
                     /old_locus_tag="pgaptmp_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGV7849527.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(83880..85007)
                     /gene="zapE"
                     /locus_tag="ID725_RS04335"
                     /old_locus_tag="pgaptmp_000867"
     CDS             complement(83880..85007)
                     /gene="zapE"
                     /locus_tag="ID725_RS04335"
                     /old_locus_tag="pgaptmp_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGV7849528.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            85201..85599
                     /gene="zapG"
                     /locus_tag="ID725_RS04340"
                     /old_locus_tag="pgaptmp_000868"
     CDS             85201..85599
                     /gene="zapG"
                     /locus_tag="ID725_RS04340"
                     /old_locus_tag="pgaptmp_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGV7849529.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            85753..87120
                     /gene="degQ"
                     /locus_tag="ID725_RS04345"
                     /old_locus_tag="pgaptmp_000869"
     CDS             85753..87120
                     /gene="degQ"
                     /locus_tag="ID725_RS04345"
                     /old_locus_tag="pgaptmp_000869"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGV7849530.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            87210..88277
                     /gene="degS"
                     /locus_tag="ID725_RS04350"
                     /old_locus_tag="pgaptmp_000870"
     CDS             87210..88277
                     /gene="degS"
                     /locus_tag="ID725_RS04350"
                     /old_locus_tag="pgaptmp_000870"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGV7849531.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(88341..89279)
                     /gene="mdh"
                     /locus_tag="ID725_RS04355"
                     /old_locus_tag="pgaptmp_000871"
     CDS             complement(88341..89279)
                     /gene="mdh"
                     /locus_tag="ID725_RS04355"
                     /old_locus_tag="pgaptmp_000871"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGV7849532.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            89714..90184
                     /gene="argR"
                     /locus_tag="ID725_RS04360"
                     /old_locus_tag="pgaptmp_000872"
     CDS             89714..90184
                     /gene="argR"
                     /locus_tag="ID725_RS04360"
                     /old_locus_tag="pgaptmp_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGV7849533.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        22272 a        24232 c        22403 g        21385 t
ORIGIN      
        1 aacatatatt cacattaaat atgattatgt acttgttaca aggataaggt tataaatgaa
       61 taaagttaca aaaacagcta ttgcgggctt acttgcgctt ttcgcaggta atgccgctgc
      121 aaccgatggt gaaatagttt ttgatggcga gattttaaaa tccgcttgtg aaatcaatga
      181 ctctgataag aaaattgaag ttgctcttgg tcactataat gccgaacagt ttcgttctgt
      241 aggcgatcgt agcccaaaaa ttccattcac tattccttta gttaactgcc cagtgactgg
      301 gtgggagcac gataacggca acgttgaagc gtctttccgt ttatggctgg aaacgcggga
      361 taatggtacc gtccctaact tccctaactt ggcaaaagtg ggctcttttg ctggcacagc
      421 agcaacaggt gtgggtatcc gtattgacga tgcggaaagc ggaaacctta tgccactaaa
      481 tgctatgggc aatgataaca cagtttatca gatccctgcg gactccgctg gaattgtcaa
      541 tgttgacctc atcgcttact acgtatcaac cgttgaagca tccgaaatca ccccagggga
      601 agcagacgct gtcgttaacg taacgctgga ttaccgttaa tacgttacgg cgttatctga
      661 cctttcagat aacgcccttt tccttcctat ttctcgttgt atcaggttga aaaataacta
      721 tgtcaaaacg aacattcgcg atgatattta ccttgttgtg tagcttctgt atcggccagg
      781 cgcttgcagg gggtatcgtt ttacagcgaa cgcgagtgat ctatgatgcc agccgcaaag
      841 aggctgcgtt acctgtcgca aacaaaggcg cagaaacacc ttatttactg caatcatggg
      901 tagataatat agatggtaca agccgtgccc catttattat aaccccaccg ctaattcgtc
      961 ttgaagctgg cgatgattca tcactgcgaa ttattaaaac agctgataac ctgcctgaaa
     1021 ataaagagtc gctgttctac attaatgttc gtgccattcc agcaaagaaa aaatcagatg
     1081 atgttaatgc taacgagttg acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc
     1141 ccgcacacct gaagggacgg gtaagcgatg cgtggaaatc actggaattt aaacgtagtg
     1201 atcattcact caatatatat aacccaactg aatattacgt cgtatttgcc ggactagcag
     1261 tcgataaagc cgatctcaca agcaaaattg aatatatcgc gcccggagaa cataaacagt
     1321 taccacttcc agcatctggc ggaaagaacg tgaaatgggc tgcgatcaat gattatggcg
     1381 gcagttccgg gacagaaact cgtccactgc aataacaaat ataaaaacac aggtcatcag
     1441 ggaatgccac aacgacacca ccagggacat aaacgcacac cgaaacagtt ggcgctcatt
     1501 atcaaacgct gtttgccgat ggtgctcact ggcagcggca tgctttgcac taccgctaac
     1561 gccgaagagt attatttcga ccccattatg ctggaaacca caaaaagtgg tatgcaaaca
     1621 accgatctgt cacgtttttc aaaaaaatac gcacaactac caggaactta tcaggttgat
     1681 atctggctga ataaaaagaa ggtttcacag aaaaaaatta catttaccgc caatgcagag
     1741 caacttctgc agccacagtt tacggtagaa caactacgtg agctgggtat taaggtggat
     1801 gaaatcccgg cgctggctga aaaagatgac gatagcgtga tcaactcgct tgaacaaatc
     1861 attcccggta cagctgctga atttgatttc aatcatcagc gacttaattt gagcattccc
     1921 caaattgcac tgtaccgtga tgcaagaggt tacgtctccc cttctcgttg ggacgatggt
     1981 ataccaacgc tgtttaccaa ctactcgttt acaggttctg ataaccgtta ccgccagggc
     2041 aatcgtagcc aacgacagta cctaaatatg caaaatggtg ccaattttgg cccctggcga
     2101 ttacgtaact attctacgtg gacacgcaac gatcaggcgt caagctggaa cactatcagt
     2161 agttatttac aacgtgatat caaggcgttg aagtctcagt tgcttctggg agaaagcgcc
     2221 accagcggca gtattttttc cagctacaac tttactggcg tgcaactcgc ttccgacgat
     2281 aatatgttgc caaacagcca gcgcggattt gccccaacgg tacgcggtat cgcaaacagt
     2341 agtgcaatcg tgactatcag gcaaaatggt tatgtgatct atcaaagcaa cgtgccagcg
     2401 ggtgcctttg aaattaacga tctctacccc tcttccaaca gcggcgattt agaagtcacg
     2461 attgaagaaa gtgacggtac gcaacgtcgc tttatccagc cttattcttc attacccatg
     2521 atgcagcgac ctgggcatct aaaatatagc gcgaccgctg gacgctatcg cgctgatgca
     2581 aacagtgata gcaaggaacc cgaatttgct gaagccacgg caatatatgg tttgaataat
     2641 acttttacgc tgtatggcgg cctgctcggt tctgaagatt attatgcgct ggggatcggt
     2701 atcggcggca cacttggcgc actgggcgcg ttgtcgatgg atatcaacag agctgacacc
     2761 caattcgata accagcactc ttttcatggc tatcaatggc gtacgcagta catcaaagat
     2821 atcccggaaa ccaacaccaa tatcgctgtc agctactatc gctataccaa cgatggctat
     2881 tttagttttg atgaagccaa tacccgcaat tgggactata acagtcgcca aaaaagtgaa
     2941 attcaattca acatcagcca gacaatattt gatggggtaa gtctgtatgc ctccggttca
     3001 cagcaagact attggggcaa taacgagaaa aacaggaata tctctgttgg ggtttccggc
     3061 cagcaatggg gaattggtta cagcctgaat tatcaataca gccgctacac tgatcaaaat
     3121 aatgaccgcg cactctcttt gaatctcagt attccgttag aacgctggtt accgcgtagc
     3181 cgggtttcct atcagatgac cagccagaaa gatcgcccaa cccaacatga aatgcgtctt
     3241 gatggctcac tgctggatga tggtcgcctg agctatagtc tggaacaaag tctggatgac
     3301 gataacaacc ataacagtag cgtgaacgcc agttaccgtt caccttatgg aaccttcagt
     3361 gccggataca gttacggtaa tgacagtagc caatacaatt acggcgttac cggcggcgtg
     3421 gttatccatc ctcatggtgt gacactctcg caatatctgg gcaacgcttt tgcgcttatt
     3481 gatgctaacg gggcatctgg cgtgaggata caaaactatc cggggattgc tactgatccc
     3541 tttggctatg cagtggttcc ttatctcaca acttatcagg aaaaccgtct ctcggtagat
     3601 actacgcagc tgcccgataa cgtcgatctt gaacaaacaa cacagtttgt ggtgcccaac
     3661 agaggtgcaa tggtagcggc gcgtttcaac gccaatatcg gttatcgcgt acttgttaca
     3721 gtcagcgatc gcaacggtaa accgttgccc tttggcgctc ttgccagcaa cgatgatacg
     3781 gggcaacaaa gtatcgtcga tgagggcggc atactatatc tctctgggat atcgagtaaa
     3841 tcacaaagct ggactgtacg ctggggaaat caggcagatc aacaatgtca gtttgctttt
     3901 agtacaccgg attcagaacc aacaacctct gtattacaag gcacagcgca gtgccattaa
     3961 ggataaaaaa atgaaaagag cgcctcttat aacaggactt ttgttgatat ccacatcctg
     4021 cgcttatgcc tcctcagaag ggtgtggagc tgacagcact agcggtgcga caaattacag
     4081 cagtgtggtt gatgatgtta cggtgaacca gacagataac gtgacaggac gggagtttac
     4141 ctctgcaacg ctaagtagca ctaactggca atacgcctgt tcctgctctg cgggtaaggc
     4201 agttaaactt gtctatatgg tcagccccgt acttaccacc actggacatc agacaggata
     4261 ttacaaactc aatgacagcc tggatattaa aaccatgtta ttaaggttaa ttttggattc
     4321 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
     4381 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
     4441 atcgacggta caactggggt agtagaccag tacaacctgg tcgccagacg aagaagttca
     4501 gacaatgcgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
     4561 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
     4621 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcacagcc
     4681 accattaccg tcatcgtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
     4741 gcactcttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccgtg
     4801 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     4861 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     4921 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     4981 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     5041 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     5101 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     5161 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     5221 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     5281 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     5341 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     5401 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     5461 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     5521 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     5581 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     5641 attgcgtaaa aaaaatatca ctggatacat tatggtaccc tcaacatttt ctcgtttgaa
     5701 agccgcgcgt tgtctgcctg ttgttctggc agccctgatt ttcgtcggtt gtggcaccca
     5761 tactcccgat cagtccactg cttatatgca gggcacggcg caggctgatt ctgcctttta
     5821 tcttcagcag atgcagcaaa gctctgatga taccaggatc aactggcaat tactcgccat
     5881 tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt gagttgttta accaactacc
     5941 gcaagaactg aacgattctc agcgtcgcga gaaaacactg ctggcggtag agattaaact
     6001 ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg aaaattacac ccgccgattt
     6061 agaacaaaac cagcaagccc gttactggca ggcaaaaatc gatgccagcc aggggcgtcc
     6121 ttccattgat ttactgcgcg cgttaattgc tcaggaaccg ctgcttggcg cgaaagaaaa
     6181 acagcagaat attgatgcca cctggcaggc gctctcctcc atgactcagg aacaggcgaa
     6241 tacgctggtg atcaacgccg acgaaaatat tctgcaaggc tggctggatc tgcagcgcgt
     6301 ctggtttgat aaccgtaacg atcccgacat gatgaaagcc gggatcgccg actggcagaa
     6361 acgttatccg aacaatccgg gcgcgaaaat gctgccaacg cagttggtta acgtaaaagc
     6421 gtttaaacca gcctcgacca acaaaatcgc cctgctgttg ccactgaatg gccaggcagc
     6481 ggtatttggt cgcactattc agcaaggctt tgaagcggcg aaaaatatcg gcactcagcc
     6541 agtggcggct caggtagctg ccgcacctgc cgcagacgta gcagaacaac ctcagccgca
     6601 aactgcggat ggcgttgcca gcccggcaca agcctcggtt agcgatctga ccggtgatca
     6661 gcctgcggcc cagccggtgc ctgtaagcgc cccggcgaca agcaccgcag cggtaagcgc
     6721 acccgcaaat ccatccgcag agctgaaaat ctacgatacc tcatcacaac cacttagcca
     6781 gatcttaagc caggttcagc aggatggcgc gagtattgtg gttggtccgt tgctgaaaaa
     6841 taacgttgaa gagttgctga agagcaacac tccgctgaac gtactagcac tgaaccagcc
     6901 ggagaatatc gaaaatcgcg tcaatatttg ttacttcgcg ctttcaccgg aagacgaagc
     6961 gcgcgatgca gcgcgtcata ttcgtgacca gggtaaacaa gcgccgctgg tgctgatccc
     7021 acgcagttca ttgggcgatc gcgtagccaa tgcgtttgcg caagagtggc agaaattggg
     7081 cggcggcacc gttctgcaac aaaaatttgg ttccaccagc gaattacgcg cgggtgttaa
     7141 cggcggttct ggtattgctt taacgggtac cccgattact cccagagcga caaccgactc
     7201 cggcatgacg accaacaatc caacgctgca aaccacgcca accgatgacc agttcaccaa
     7261 taatggcggt cgtgtcgatg cggtgtacat tgtggcaacg ccgggtgaaa tcgcttttat
     7321 taaaccgatg atcgccatgc gtaacggtag ccagagcggt gcaacgctgt acgccagctc
     7381 ccgcagtgcg caagggaccg ctggcccgga tttccgtctg gagatggaag gcttgcagta
     7441 cagcgaaatc ccgatgctgg cgggcggtaa tctgccgtta atgcagcagg cactcagcgc
     7501 ggtgaataac gattattcac tggctcgcat gtatgcgatg ggcgtcgatg cctggtcgct
     7561 ggcaaatcat ttctcacaaa tgcgccaggt tcagggtttt gaaatcaacg gtaataccgg
     7621 aagcctgacg gctaacccgg attgcgtgat taacaggaag ttatcatggc tacagtacca
     7681 acaaggtcag gtagtccccg ccagttaacc accaaacaga ccggcgatgc gtgggaagta
     7741 caagcgcgtc gctggctgga aggcaaagga ctgcgatttg tcgccgctaa cgtgaacgag
     7801 cgtggcggcg agatcgatct gataatgcgt gaaggccgga ccaccgtttt tgtcgaggtg
     7861 cgttaccgcc gttctgcgct ttatggcggc gcggcagcca gtgtgacccg cagcaaacaa
     7921 cacaaattat tacagactgc ccgcttgtgg ctcgcgcgtc ataatgggag ttttgatact
     7981 gtggattgcc ggttcgatgt ggtagccttc accgggaatg aggttgagtg gattaaggat
     8041 acctttaatg accactcata attaaggttt aaggattagc gtgcaagaaa gaattaaagc
     8101 ttgcttcact gaaagcattc aaactcaaat tgcggcggca gaggcgcttc cggatgccat
     8161 ctcccgtgcg gccatgacgc tggttcagtc tctgctcaat ggcaacaaaa tcctctgttg
     8221 tggtaatgga acttccgctg ccaatgcaca gcattttgct gccagcatga tcaaccgttt
     8281 cgaaacggag cggcccagct tacctgccat tgcactaaat accgataatg ttgtcttaac
     8341 ggcgattgcc aacgatcgct tacatgatga agtgtatgca aaacaggtgc gggcgctggg
     8401 gcatgcggga gatgtattgt tagccatttc cacccgtggc aacagccgcg atatcgtaaa
     8461 agcagtagaa gctgccgtta cgcgtgatat gaccattgtg gcattgaccg gttatgacgg
     8521 cggtgaactt gcaggtttgt tagggccaca ggatgtggag atccgcatcc cttcgcatcg
     8581 cagtgctcgc attcaggaaa tgcatatgct gacggtaaat tgcctgtgcg atctgatcga
     8641 taacacgctt ttccctcacc aggatgatta aggagaatac atgaaggcat tatcgccaat
     8701 cgcagtcctt atttccgcgc tgctgttgca aggttgtgtt gccgctgccg tagtgggtac
     8761 tgctgctgtg ggtaccaaag ccgcaactga cccacgcagt gtcggcaccc aggtggacga
     8821 tggtaccctg gaagtgcgcg tgaacagcgc attgtcgaaa gacgaacaga ttaagaaaga
     8881 agcgcgcatt aatgtaacgg cctatcaggg caaagtgctg ctggttgggc agtcaccaaa
     8941 cgctgaactt tcggctcgcg ccaaacagat tgctatgggc gtagacggtg ccaacgaagt
     9001 gtataacgag attcgtcagg gccagccgat tggtctgggc gaagcgtcta acgatacgtg
     9061 gatcaccacc aaagtgcgtt cgcagctctt aaccagcgac ctggtgaaat cgtccaacgt
     9121 gaaagtgacc accgaaaacg gtgaagtgtt cctgatgggg ctggtgactg aacgtgaagc
     9181 gaaagcggcg gcagatattg ccagccgggt gagcggcgtg aagcgggtaa ctacggcgtt
     9241 tacgtttatt aaatagcagc cccttgtaat gcctgatgcg acgcttgccg cgtcttatca
     9301 ggcctacaac gatgcaaacc gtaggtcgga taaggcgttt acgccgcatc cgacatgttt
     9361 tccctcaaat cagaataaca gcgccagccc gccgacaatc acgcctgaca ctgccaccat
     9421 cgcccccgtc agccataagg ctttcgccgg gaacgctttg cgcagcataa tcagtgacgg
     9481 caaactcacc gccgggagcg tcatcaacaa tgccagcgcc ggagcagttc ccatacctgc
     9541 cagcatcatc gtttgtacaa tcggaatttc tgctgccgtg ggaatgacaa acaagcaccc
     9601 tgctaccgcc atcgccacca cccacatcag gctgttatcg acagcaccat cggcatgggg
     9661 gaataaccag acgcgagcgg cacccaacac cagtactgca aggatgtaaa ccgggatcgt
     9721 actccagaaa agcgtccata gcgccctgcc ccagcgacta aaaaatccgc cctgtgcttc
     9781 cggtatgtca atttcgaccg gtgcttgcgt ttgcggtgtt tcacgcaccc atttttgcac
     9841 cagcgtcgca atcagcaaca ccatcaccag cccggccacc agacgaatcg ccgcaaaacc
     9901 ccagccgagg acaaacccca taaacaccag cgtcgccggg tttaacaccg gattgcccat
     9961 ccagaatgcc agcgcaccgc ccatcgacac ctgttggcga cgcattcccg ccgcgaccgg
    10021 agccgcacag caggtacaca tcatgcctgg cagcgaaaac agcgttccca gcagcgtgcc
    10081 gcgaaagcgc gattgcccaa gcgtacgcaa caaccagtca cgcgggatca gcacctgaat
    10141 caacgaaccg aggatcaccc ccagcaccgc cgctttccat accgcgagga aatagatcat
    10201 cgcgtaatcc aacgccgcct gccatggatt agcatccgcc tgcgcaagga tagatttacc
    10261 gatactgtgg gtttcggcag cagtaaacgc tttgccgtag taaggttgcc atttcacata
    10321 ccagagaccg gcaatgacaa cgagaaagaa aagcgcgggt ttccaccact gaatgggcgt
    10381 tgccgcctga gatgaagact gaccagtcat agcattcccc aggaataagt tatgataatt
    10441 gagcgcgtga atattacgct cactatcaat tcttgggaat aattattcag ctcttttgcg
    10501 taattctgaa gagcttaaaa tcgtcacacc ttcatgctcg ggccgcatcg cctctgccag
    10561 catgacccgc gccacgtctc tcgcatcaat ggatttccag ttaccgggta acaaacggaa
    10621 cagcggcgca aaaagcgttt cgttcatccg ctgtttgcta cgatcgccca gtaacatcga
    10681 cgggcgagca atggtcaatt tcggccagtt ctgggcgatt aatgcttctt ccatctcccc
    10741 tttgacgcgg ttatagaaaa acggcgagtg ggcattggca cccatagcac tgaccaccag
    10801 catatgctgc gcgcccagtc gccgcccggt taatgcggta tccactacca gcgtgtaatc
    10861 ggcatgaata aacgcctctt tgctccccgc ttctcgccgc gtggtgccga gacaacaaaa
    10921 cacaatgtcg atgggatcgg tgacctgcgc cagcgcgtca gtcagttgcg gatcatgggg
    10981 attaaatacc ccgggcatat cgcccaacgg acgtcgcgtc ggcgcggcta tggcgttaac
    11041 tttcggttcg ttaatcaaca tccgcagcag gtgaccgccc accagccccg ttgcgcctgt
    11101 aatcagtacc tgactcatct tcgctccttt acagaattgt ctgccttgcg ctccacggct
    11161 catgcaccag gcttaataga ccatgaggta attatccccg attgtgggaa attcgcctca
    11221 tccaatgcaa caacgcggag gaagcatgag taagaaaatt gccgttttaa tcactgatga
    11281 atttgaggat tcagaattta cttcacccgc agacgagttc cgtaaagccg gacacgaagt
    11341 gattaccatt gaaaaacaag cgggtaaaac ggtgaaaggc aaaaaaggag aagccagcgt
    11401 gaccatcgat aaatccatcg atgaagtgac gccagcggag tttgatgccc tgttgctacc
    11461 gggcggtcat tcaccggatt atctgcgtgg cgacaaccgt tttgtcacct ttacccgtga
    11521 ttttgtgaat agcggcaaac ctgttttcgc catctgtcac ggtccgcagc tgctgatcag
    11581 cgccgatgtg attcgggggc gcaaactgac cgcggttaaa ccgatcatta ttgatgttaa
    11641 aaacgcgggc gcggaatttt acgatcagga agtcgtggtc gataaagatc agctggttac
    11701 cagccggaca ccggacgatc tgccagcgtt taaccgcgag gcgttacgcc tgctcggtgc
    11761 ctgagccgcg tagccaaatc atttttttac caaagcccag cgtgttgtcg gtgaatttga
    11821 tttcatcggg ccggatttcc cacaccgggg ccgacagcat tctggcaacc ggaaagcgac
    11881 gattgtacgc cttgcgcgcg aggtcgcttt cctcaccttc cagcctgcgg atctcacctt
    11941 taaactgcac gccgcgaatt aacgctaccg ttttcggctg accgtttacc gttccggcaa
    12001 ccgcagcctg cggcccacgc atctgcgcgt ggcgcgtttt ttcttccgtc aaaatgtaga
    12061 aggcgacttt ctgcacatca aacagataaa aggcattggc gcaccagagt tccccttcct
    12121 gctgcacaca ccaggtgaca acatgttgtt ttgccagcca acggctgatg gcggtgagtg
    12181 tttccattgc tgttctcttt tatactgtgg gcctgacctt aacatgcgac gcagacgatg
    12241 acaccctggt ttctttactt gatccgtacc gccgacaata agctttatac cgggatcacc
    12301 acggatgtcg aacgccgcta tcagcagcac caaagcggca aaggggcgaa agcactgcgc
    12361 gggaaaggag aactgacgct ggcgttttct gcgccagtgg gcgatcgttc gctggcgtta
    12421 cgggcggaat atcgcgttaa gcaactgacc aaacggcaaa aagagcgtct ggtagcggaa
    12481 ggcgcagggt ttgcaaagct gttaagcagt ctgcaaaccc cggagattaa aagcgattga
    12541 aatgctcgtg atactcaacc agaccagtta cgccattcaa cgcgtcatcc gctaaacgat
    12601 gtacctggaa ggcgctttca gtgcccggcc agcggcaacg cagatcgtga tgcgccgcca
    12661 gttcaaagcc gaaacggctg tatagcgccg gatcgcccag cgtcaccact gcggcatagc
    12721 cgaactcatt aagcgaatcg agtccttcat agaccagttg gcgtgccagc ccctgtccac
    12781 ggtatttttc atctaccgcc agcggtgcca tgccgaccca ttgcaggtct tcgccctgca
    12841 catcaaccgg actaaatgcc acatagccaa tgacctgacc ttcgtcatct gtcgccacca
    12901 gccccagcgt cagaaagcca tcttcacgca gatcgtgaac cagcttcgct tccgcatcac
    12961 tttcgaatga acgacgcagc agggcatcaa tacccggcgc atcaatggga atttctactc
    13021 gaattagcat ggttcaccta ccgatgtctg tttggtttca ggcgcggttt tcattcccgc
    13081 ctcaacaaaa tccgccagtt gcagcagcat catgcgtaag gctttcggca tttgctccag
    13141 ttcaatggcg tccatcaggt ttttcacata cagccccagc tccgtatcgc cttcaatcac
    13201 cagccgacgc tggaagaaga gcgtatccgg atcttgttta cgcgccgcaa tcatcagcag
    13261 atcgctggcg tcggcactaa aactcacatc agcttgcgcg ttctggctga ccaccagttt
    13321 gccattcacc accgaggtaa accattgcag gtcaatatca cgcacatgaa tacttaacca
    13381 gcggccttca agaaactcca gctcaccatc atccagcgcc tggcggaatt gccagcttaa
    13441 gacctgctca agaacctggc gttttagcgc aaatggcgtc agttttaccg gtacactcaa
    13501 cagagatggc cccaaatgca caatacggga acgcagttta tccaacacga gttttactcc
    13561 ctgtttcaac aatcatccta ttttgccata tcagaaaaat aacatagcgg tataaatcaa
    13621 caattccata tgaaattgct gctaccacca atacaacttt aactgcctta aatcaaaaat
    13681 tgtcgcagca aggttaacta aaatcccagt tcgttaacat ttttgcgttt tgatagcgca
    13741 accttcagga aaaattatgg agctgctctg ccctgccgga aatctcccgg cgcttaaggc
    13801 ggccatcgaa aacggcgcag atgctgttta tatcgggcta aaagatgata ccaatgcccg
    13861 tcacttcgcc ggccttaact ttaccgagaa aaaattgcag gaagcggtga gttttgtcca
    13921 tcaacatcgc cgcaaacttc acatcgcgat taacactttt gcgcatccgg acggttacgc
    13981 ccgttggcag cgtgccgtgg atatggcggc gcagctgggt gccgacgcgc tgatcctcgc
    14041 cgacctcgcc atgctagagt acgccgccga gcgttacccg catattgagc gtcatgtgtc
    14101 agtgcaggct tcggcgacca atgaagaggc gattaacttt tatcatcgcc attttgacgt
    14161 tgctcgcgtg gtgctgccgc gcgtgttgtc gattcatcag gtgaaacaac tggcacgggt
    14221 cacacctgtc ccgctggaag tctttgcttt cggcagcctg tgcattatgt cggaaggtcg
    14281 ttgctatctg tcgtcgtatc tgacgggtga gtcgcccaac accgtgggcg cgtgttctcc
    14341 ggcccgtttc gtgcgctggc agcaaacgcc gcaggggctg gaatcccgcc tgaacgaagt
    14401 gctgatcgac cgttatcagg acggcgaaaa cgcaggttat ccgacgctat gtaaagggcg
    14461 ttatctggtg gacggcgagc gctatcacgc gctggaagaa ccaaccagtc tcaataccct
    14521 ggaactgctg ccggagttaa tggcggcgaa tattgcttcg gtgaaaattg aaggccgcca
    14581 acgtagcccg gcgtatgtca gccaggtggc gaaagtctgg cgtcaggcta tcgaccgttg
    14641 taaggccgat ccgcaaaact tcgtaccgca aagcgcgtgg atggagacgc tcgggtcgat
    14701 gtccgaaggc acgcagacca cccttggcgc gtatcaccgt aaatggcagt gagaagagca
    14761 atgaaatatt ccttagggcc agtgctgtgg tactggccaa aagagacgct ggaagatttt
    14821 tatcagcagg ccgccaccag cagcgccgac gtgatttatc ttggcgaagc ggtttgcagc
    14881 aagcgccggg caaccaaagt tggcgactgg ctggagatgg caaaatcgct cgccggaagt
    14941 ggtaagcaga ttgtgctctc cacgctggcg ctggtgcagg catcatctga actgggcgaa
    15001 ctgaaacgct atgttgagaa cggtgagttt ctgattgaag ccagcgatct cggcgtggtg
    15061 aatatgtgcg ctgaacgcaa actgccgttc gtcgccggac acgcgctgaa ctgctataac
    15121 gcggtgacgc tgaaaatatt gctcaaacag ggcatgatgc gctggtgtat gccggtggag
    15181 ctttcccgcg actggctggt gaatctgctt aatcagtgcg atgagctggg cattcgcaac
    15241 cagtttgaag ttgaagtcct gagctacggt catctaccgc tggcctactc cgctcgctgc
    15301 tttaccgcgc gttcggaaga ccgcccgaaa gatgagtgtg aaacctgctg cattaagtat
    15361 ccgaacgggc gcaacgtgct gtcgcaggaa aaccaacaag tgtttgtact caatggcatt
    15421 cagaccatga gcggctacgt ttacaacctc ggtaacgagc tggcatccat gcagggcctg
    15481 gttgatgtgg tccgcctgtc accgcagggt actgacactt tcgcgatgct cgacgccttc
    15541 cgcgctaatg aaaatggcgc tgcaccactg ccgttgacgg cgaacagcga ctgtaacggc
    15601 tactggcggc ggctggcagg actggagctg caagcctaag taaatagctc actttgttaa
    15661 caactttaac tactctttaa tgcagtatta aagattaatc ggtaacaaag tgagctgtta
    15721 tgactgataa aaccattgcg ttttcgctac tcgatctggc ccccattccc gaaggttctt
    15781 cagcgcgaga tgcattctcc cactctctcg atctcgcccg tctggctgaa aagcgcggct
    15841 atcatcgcta ctggctggca gaacaccaca atatgactgg cattgccagt gctgccacgt
    15901 cggtattgat tggctatctg gcggcgaata ccaccacgct gcatctgggg tctggcggcg
    15961 tgatgttgcc taaccactca ccgttggtca ttgcagaaca gttcggcacg ctcaatacac
    16021 tctatccggg gcgaatcgat ttggggctgg gtcgtgctcc gggtagtgac cagcgaacca
    16081 tgatggcgct acgtcgtcat atgagcgggg atattgataa tttcccccgc gatgtcgcgg
    16141 agctggtggc ctggtttgac gcccgcgatc ccaatccgca tgtgcgcccg gtaccaggct
    16201 acggcgagca aatccccgtg tggttgttag gctccagcct ttacagcgcg caactggcgg
    16261 cgcagcttgg tctgccgttt gcgtttgcct cacacttcgc gccggatatg ttgttccagg
    16321 cgctgcatct ttatcgcagc aacttcaaac cgtcggcacg actggaaaaa ccatacgcga
    16381 tggtgtgcat caatattatc gctgccgaca gcaaccgcga cgctgaattt ctgtttacct
    16441 caatgcagca agcctttgtg aagctgcgcc gtggcgaaac cgggcaactg ccgccgccga
    16501 ttcaaaatat ggatcagttc tggtcaccgt ctgagcagta tggcgtgcaa caggcgctga
    16561 gtatgtcgct ggtgggcgat aaagcgaaag tgcgtcatgg tttgcagtcg atcctgcgcg
    16621 aaaccgacgc cgatgagatt atggttaacg ggcagatttt cgaccaccag gcgcgcctgc
    16681 attcgtttga gctggcgatg gatgttaagg aagagttgtt gggatagtgt gtcttaacgc
    16741 gggaagcctt atccgagctg gcaacgctgt cccacgtagg cctgataagc gaagcgcatc
    16801 aggcattgtg taggcagcag aaatgtcgga taaggcccgc tgattactga tacaccggca
    16861 gcaaattaaa gctcgataag atatggacca gtgcgttgcc aacgccaaat accagaatca
    16921 gcgtaatcat cggcttgccg ccccagacgc ggaatttcgg gctgccaaag cgtttacgcg
    16981 atgcgcgggc cagcagtgcc ggaacaattg ccgcccagat ggtagctgct aaaccagcat
    17041 aaccaatggc gtacaggaat ccgttcggga acagcagacc acccacaaca ggaggggcaa
    17101 aggtcagcaa tgccgttttc aagcggccca cagccgagtc gtcgaaacca aacagatctg
    17161 ccagatagtc aaacaaaccc agcgttacgc cgaggaacga actcgctacc gcaaagtttg
    17221 agaacacgac cagcagcaga tccagactac ggctgttcag tacgccgctt aacgcctgta
    17281 ccagcacatc aatattaccg cccttctctg caatatcgat aaactccgga cgcgggatgt
    17341 tacccatcgt cgccagcaac cagatggtat acagcgccag cgccatcagc gtaccgtaca
    17401 ccagacattt cacgatggtt ttcggatctt tgccgtaata cttcatcagg cttggcacgt
    17461 taccgtgata accaaacgat gccagacaga acggcaaggt catcaacaga tacggtgcat
    17521 aagacgcatt gctttcagcg acgttgaaca atgtcgcagg ctgcacatgc cccagcaggc
    17581 taccgaaggt gaggaagaag gtaatgactt tcgcccccaa tacaatcgct gtcatgcgac
    17641 tgacggcttt agtgctcaac cacaccacaa acgctaccag caatgcaaaa ccaaaacccg
    17701 ccgcccgtgc cgggacgttt agtgacatct ctgcgaaggt gtgatgcaga atcgaaccac
    17761 tggcagaaat ataggcatag gtcaggatat agagcacaaa ggcaatggaa atgccgttga
    17821 ccacgttcca gcctttgccc agcaaatctt tggtgatggt gtcaaaactc gaaccgattc
    17881 tgtaattcag gttagcttcc agaatcatca agccggaatg cagcatacag aaccaggtaa
    17941 agatcagcgc cgccattgac cagaaaaacc acgccccgga catgaccact ggcagagaaa
    18001 acatccctgc gccaataatg gtgccgccga taatcaccac gccgccaagc agcgacggtg
    18061 acgtttgggt ggtggttagt gttgccatga gggcttctct ccagtgaaaa atagtgcgac
    18121 tgcgttgtta tgtattgcac tgtaccagta cgcgagtaca aaagacagaa aaaaagcccc
    18181 gatggttcaa atcggggctg tatatattat tttacagact gtgttcgctg ttcagcgatg
    18241 attacgcatc accaccgaaa cgacgacgac cggtagagtc atcacgacgc ggagcgcggc
    18301 cttcacgacg ttcgccgcta aaacgacgac catcaccacg accaccttca cggcgttcac
    18361 cgctgaagtt acgaccacct tcacgacgct cgccactgaa accacgacca ccgccacgac
    18421 gctctccgcc agtatgcggc tgtgcatcgc ccagtaattg catattcatc ggcttgttca
    18481 gaatgcgagt acgtgtaaag tgttgcagca cttcacccgg catacctttc ggcagttcga
    18541 tggtggagtg agaagcaaac agcttaatgt taccgatgta acggctgctg atgtcgcctt
    18601 cgttagcaat cgcaccaacg atatgacgaa cttcaacacc atcatcgcgg cccacttcaa
    18661 tacggtacaa ctgcatgtca ccaacatcac gacgttcacg acgcggacga tcttcacggt
    18721 cgccacgcgg accacggtcg ttacgatcgc gcggaccacg gtcatcacgg tcacggaatt
    18781 cacgtttcgg acgcatcggc gcatctggcg gaacaatcag agtacgttca ccctgtgcca
    18841 ttttcagcag tgcggcggcc agagtttcga gatccagctc ttcaccttct gcagtcggct
    18901 gaattttgct cagcagtgcg cggtattgat ccagatcgct gctttccagc tgctgctgta
    18961 ctttagcggc gaatttttcc agacggcgtt tgcccagcag ttctgcgttc ggcagttcta
    19021 cttccggaat agtcagcttc atagtacgtt caatgttgcg cagcagacga cgctcgcggt
    19081 tctcaacgaa cagcagcgcg cggccagcac gacccgcacg accggtacga ccgatacggt
    19141 gaacgtaaga ctcagaatcc atcgggatat cgtagttaac caccaggctg atacgctcaa
    19201 cgtccaggcc acgggctgca acgtcggtcg caatcaggat gtccagacga ccatctttca
    19261 ggcgttccag tgtctgctca cgcagcgcct ggttcatgtc accgttcagc gcggcgctgt
    19321 tgtagccgtt acgctcaaga gcttcagcca cttccagagt cgcatttttg gtacgaacga
    19381 agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg taccagtgct tcgtttttac
    19441 gcatacccca gacagtccaa tagctctggc tgatgtcagg acgggtagtc acgctggact
    19501 gaatgcgcac ttcctgcggc tctttcataa agcggcgggt aatgcgacga atcgcttccg
    19561 gcatggttgc agagaacaga gcggtctgat gaccttccgg gatctgcgcc ataatagttt
    19621 caacgtcttc gatgaagccc atgcgcagca tttcgtcagc ttcatccaga accagaccgc
    19681 tcagtttaga gaggtccaga gtgccacgtt tcaggtggtc cagcagacgg cccggagtac
    19741 cgacaacgat ctgcggcccc tgacgcaggg cgcgtaattg cacgtcataa cgctggccgc
    19801 cgtacagggc aaccacattt acgccgcgca tgtgtttaga gaaatccgtc attgcttcag
    19861 caacctgtac cgccagttcg cgggtcggtg ctagcaccag aatctgtggt gctttcagct
    19921 caggatcaag attttgcagc agaggtaaag agaatgctgc agtttttccg ctccccgtct
    19981 gggccatacc cagtacgtcg cggccattca gcagatgtgg aatacactcc gcctgaattg
    20041 gagatggttt ttcgtaaccc agatcgttaa gggcttcaag gataggagcc ttcaggccca
    20101 gatctgcaaa agtggtttcg aattcagcca tgtagtacgt gtgcctcaaa attaatggcg
    20161 gccagtctac ataactcatc atgaaattga tcagcaattt tcattgaaaa gtgtgaaccg
    20221 gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc cgttaaggtg atggcaatca
    20281 aaaaagatta cgggctgatg tgtacgtcag ctattgctgg tccgattctg ccaggtcatc
    20341 ttggtcctgg cccaggagcg ataattccaa caatgcgtat cggtgctcaa caaagttatg
    20401 aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg ctgtccaaat cccccagact
    20461 taggtagtac ttacctaaat agaagttggt ttcactgaga tgctcagcga gcgaggtgtt
    20521 atccgttgcg tccgccttga gcctttccat taacgtttgt tcgctaatgt tgcccaggta
    20581 gaactcgaca atgttccatc cccactgttc cttatccgat ttttcgaagt gctgtttcaa
    20641 cacttcttta gcctgcttct catcgagctt ctgctcggcg agataaagcc acagactacg
    20701 gaaaggatca ttgggatcgt cttgataaaa cgccagcaga tcatcttgcg ctaacttgtc
    20761 acgaccgccg taatataatg cgatcccgcg attcaagtgc gcgtagttgt aagttggatc
    20821 aagctcaagt acagaatcaa acgcttcata ggcagcatca aaattgcctg cctgcgttaa
    20881 atatatgcct aagtaattga atacttcagg catatccggt cggattgcca gcgcttgcga
    20941 aaaatcgtta cgcgctaatg ccctcagacc gagactatca tacaacactc cgcgctcata
    21001 taaaagctgt gcgcgttcgt catcggttaa agcccgactg gcaaggattt gttccatacg
    21061 tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt accgcgagga cttcactttt
    21121 acgccaggaa gtattactgc atcctgcaag cgtaagtgct gtcgcaacga aacaccagcg
    21181 caaaaaaggc ttcatttccc actcccgaag accacggttg aatgaacgtc ctgttcccgg
    21241 ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc gcagcggagg gcaaatggca
    21301 accttactcg ccctgttcag cagccggagc ttccggtgct gcagcaggtt gagactgctc
    21361 agtcgcttct ttaatgctca gacggatacg gccctggcga tcaacttcca gaactttcac
    21421 cggtacttcc tgacccatct gcaggtaatc ggtcactttc tcaacgcgtt tgtcagcgat
    21481 ttgagagatg tggaccagac cttctttacc gccgccgatg gcaacaaatg cgccgaagtc
    21541 aacgatacgg gtcactttac cagtgtagac gcggcccact tcaatttctg cagtgatctc
    21601 ttcgatacga cgaatagcgt gtttcgcttt ctcgccatcg gtcgctgcga tcttcacagt
    21661 accgtcatct tcgatttcga tggtggtgcc agtttcttcg gtcagggcac ggatcacaga
    21721 gccgccttta ccgataacgt ctttgatctt gtccgggttg atcttgatgg tatggatacg
    21781 cggagcgaac tcagagatat cgccacgtgg cgcgttgatc gcctgttcca ttacacccag
    21841 gatgtgcaga cgcgcacctt tagcctggtt cagtgcaacc tgcatgatct ctttggtgat
    21901 accttcaatt ttgatatcca tctgcagtgc agagataccg tcgcgggaac ccgcaacttt
    21961 gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg tcagacagta caacgtagtt
    22021 gtcgccttct ttcaccagac ccattgcgat acccgcaacg gcagctttga ttggcacacc
    22081 tgcgtccatc agcgccagag acgcaccgca cacggaagcc atagaagaag aaccgttgga
    22141 ttcagtgatt tcagacacaa cacgtacggt gtacgggaat ttgtccatat ccggcattac
    22201 tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt tcacgacgct tcggagaacc
    22261 gaccatgccg gtttcgccta cggagtacgg agggaagttg tagtggaaca ggaaggtatc
    22321 ggtacgttcg cccatcagtt catcaagaac ctgcgcgtca cgagcagtac ccagcgttgc
    22381 ggtaaccagc gcctgcgttt caccacgggt gaacagcgca gaaccgtgag tacgcggcag
    22441 cacgccagta cgcacatcca gaccacggat catatctttt tcacgaccat cgatacgcgg
    22501 ttcgcctgcc agtacgcggc tacgaacaac gtttttctcg atcgcgtgca gaatttcacc
    22561 cagttcgttt tcgtccaggg tttcgtcttc agcaagcagc gtcgcgatgg tttcagattt
    22621 gatgacatca acctgcgcat aacgctcttg tttgtcggtg atgcggtaag catcgctcag
    22681 acgagcttca gccagtgcag caacgcgcgc gttcagcgct tcgtttaccg gctccggctg
    22741 ccagtcccaa cgcggtttac cggcttcttt caccagttca ttgatgttct gaataacaac
    22801 ctgctgttgt tcatgaccga acactactgc gcccagcatc tggtcttcgc tcagcagttc
    22861 agcttcagat tcaaccatca gtacagcggc ttcagtaccc gcaacaacca gatccagttt
    22921 gctctctttc agctcgtcct gagtcgggtt cagtacgtac tggtcattga tgtaacctac
    22981 gcgggcagca ccaatcgggc cattgaacgg aataccagac agagacagtg ctgcggaagc
    23041 accaatcatc gcgacgatat ccgggttaac ctgcgggtta acagaaacca cggtggcgat
    23101 aacctgaact tcgttgacga agccttccgg gaacagcggg cgaatcgggc ggtcaatcag
    23161 acgcgcgatc agggtttcgc cttcgcttgg acgaccttca cgacggaaga agctacccgg
    23221 gatacgacca gcagcgtagg tacgctcctg atagttaacg gtcagtggga agaagtcctg
    23281 acctggtttg gcttttttct ggccaacaac ggtaacaaat accgcggtgt catccatgct
    23341 aaccataaca gcggcagtag cctgacgagc catcatgccg gtttccagag tcacggtgtg
    23401 ttggccgtac tggaatttac gaacgatcgg attaagcaat gtaatatcct ttctctttct
    23461 tagacagtac cttacggcac tggtgttaat acccgatctt ctgcgcatcc tcgcgactaa
    23521 tgacaaccct aacccagctc tatgtgggta aagcctctca ttagccgcgc gaacctctgc
    23581 aacggaagat cattcatagc aacaatacat tagtttccag tgaattgctg ccgtcagctt
    23641 gaaaaaaggg gccactcagg cccccttttc tgaaactcgc aagaattagc gacgcagacc
    23701 caggcgctcg atgagccggg tgtaacgtgc tacgtcttta cgtttcaggt agtcgagcag
    23761 tttacgacgc tgagaaacca tgcgcagcag accacgacgg ctgtggtgat cttttttgtg
    23821 ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc agcagtgcta cctgaacttc
    23881 ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa acgattttag ctgttgcttc
    23941 agtacttaga gacattttaa aactccaaag tatatagaat gaaaagacgc cgatctctaa
    24001 ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa tttacgcgac gttaagcggc
    24061 agtattctac tcgtagcgac ctgttatcgc aagacggtta acattacgcc gggtattcaa
    24121 ccaccagacg acgaggcgca acgcggcctt catcgtcaat ttcgcccata ccgataaact
    24181 tgccgttctc accttccgta acgcgaacca gtccttccag cggcgcgcca gatgtacgaa
    24241 ccgggttacc atttttgaag taaacagaag acgttaacgg aagattcacc accgggtagt
    24301 ccgaagctgg actgtccatt ggcatcagta atggatcaag caactccgca gccggaatat
    24361 cctgctgttc ggcttgttcg accagttcac gcaggtgctc cagggtcacc atccgttcaa
    24421 ccggatattt acttaccgcc agacgacgca ggtaaatcac atgcgcgcca cagccgagtt
    24481 tttcacccag gtcatcaatg atggtgcgga tataagtgcc ttttgagcag tgaatttcca
    24541 gctccagctc attgccttca tggcgaataa acagcaattc ataaacggta attggacgcg
    24601 cttcacgcgg aacttcaatg ccctgacgcg catattcgta cagttttttg ccctggtatt
    24661 tcagtgctga atacatcgat gggatctgtt cgatatcgcc acggaaagta tccagtgccg
    24721 ccgccagttg ttctgcacta aaggttaccg gacgctcttc aacgatttgt ccgtcgacgt
    24781 cagaagtatc ggtacgctgt ccaagacgcg caatgacccg atagcgtttg tcggagtcca
    24841 gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat cggcaacatg ccggtcgcca
    24901 gcgggtccag cgcaccggta tgcccggcac gattggcgtt atatatacgt ttcacttttt
    24961 gcagcgcatc gttgctggac ataccctgcg gtttatccag caacaaaacg ccgttaatgt
    25021 cgcgaccgcg acgacgagga cgactcatta gtcctccttg ctgtcgtccg ggttaacacg
    25081 acgttcttcg tcatgtttga ccacgctggt caccaggttt gacatacgca tcccttcaac
    25141 cagagagttg tcgtagaaga aggtcagttc cggcacgata cgcaggcgca tcgctttacc
    25201 cagcaggctg cggatgaaac cagaagcttc ctgcaacgct ttgatgcccg ctttaaccgc
    25261 gtcttcatct ttgtcgttga ggaaagtcac atacactttg gcatacgcca ggtcgcgaga
    25321 catttcgaca ccggaaacgg tggtcatcat gcccagacga ggatctttaa tttcacgctg
    25381 caggatgaga gcgatctctt tttgcatttc ctgcgctacg cgctgcgggc gaccaaattc
    25441 tttcgccata ataaattctc ctgacaaaaa aggggctgtt agcccctttt taaaaattaa
    25501 tttcaggtgg aagggctgtt cacgttggcc tgataagacg cgccagcgtc acatcaggca
    25561 atccatgccg gatgcggcgt aaacgcctta tcccgcatgg aaccctaaaa accttaagcg
    25621 atggtacgtt ggatctcgat gatttcgaat acttcgatca catcgccagt gcggacgtcg
    25681 ttgtagttct taacgccgat accacattcc ataccgttac ggacttcgtt aacgtcatct
    25741 ttgaagcggc gcagggactc cagctcgcct tcatagataa ccacgttgtc acgcagaacg
    25801 cggatcgggt tgtgacgttt aaccacacct tcggtaacca tacagcctgc gatggcacca
    25861 aatttcggcg atttgaacac gtcacgaact tccgccagac cgataatctg ctgtttcagt
    25921 tccggagaca gcataccgct catcgccgct ttcacttcgt cgatcaggtt atagatgacg
    25981 gagtagtaac gcagatccag gctttccgct tcaatcactt tacgtgcaga ggcatcagca
    26041 cgtacgttga agccaaccag gatggcgttg gacgccgcag ccaaggtggc gtcggtttcg
    26101 gtgataccac ctacgccaga accgatgatc ttcactttaa cttcgtcagt agacagtttc
    26161 agcaaggagt cggagatcgc ttcgacagaa ccctgtacgt ctgccttcag gacgatattc
    26221 acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt tctcgagttt agatttctgc
    26281 tgacgcgcca gtttaacttc gcggaattta ccctgacgat agagtgcaac ttcacgcgct
    26341 ttcttctcgt cacgtacaac ggtaacttca tcacccgcag ccggaacacc ggacaagccg
    26401 aggatttcca ccggaatgga cggacccgct tccagcactt cctgacccag ttcgttacgc
    26461 atcgcacgaa cacgaccgta ttcgaagcca cacagaacga tatcgccctt gtgcagagta
    26521 ccttcacgta ccagaacggt agcaaccgga ccacgacctt tatcgaggaa ggattcgata
    26581 accgcaccgc tcgccatacc tttacgtacc gctttcagct ccagaacttc cgcctgcagc
    26641 aggatagcgt ccagcagttc atcgataccg gtacccgctt tcgcagatac gtgtacgaac
    26701 tggctttcac cgccccactc ttccggcagg atgccgtact gggagagttc gttcttaacg
    26761 cgatccggat cggcttctgg tttatcgatc ttgttcactg caaccaccac cggtacctgc
    26821 gccgctttcg cgtgctggat tgcttcgatg gtctgcggca ttacgccgtc gtcggcagca
    26881 acaaccagga ctacgatgtc agttgcctgc gcaccacgtg cacgcattga agtaaacgcg
    26941 gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt cagtttcaac gtggtatgca
    27001 ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcca ctttcgttga acgaatgtag
    27061 tccagcagag aggttttacc gtggtcaacg tgacccatga tggtcacaac cggagcgcgc
    27121 ggttcagccg cagctcccgt gtcacggtcg ctcattaccg cctcttccag ctcgttttca
    27181 cgacgcagga taactttgtg gcccatctct tcagcaacca gctgtgcggt ttcctgatcg
    27241 ataacctggt tgatggttgc cattgcgccc agtttcatca tcgctttgat gacctgagag
    27301 cctttaaccg ccatcttgtt cgccagttcg ccaacggtga tagtttcgcc gatcacaacg
    27361 tcacggttaa cggcctgagc aggcttctgg aagccttgct gcagcgaaga acctttacgt
    27421 tttccgccct taccgccacg tactgctgcg cgtgcttctt cacgatcagc ttttgattca
    27481 gcgtgtttgt tgcctttctt cggacgcgct gctttcgcgt tacgaccacg gccacggccg
    27541 ccttcgactt cacgatcgct ttcgtcttct gcctggcgag catgttgaga agtagtgacg
    27601 tgataatcgc tggaatcctc agtcggttcc gcgttatcag tccatttgtt ttcttccgcc
    27661 atacgacgtg cttcttcagc aacgcgacgt gcttcttctt cgagtttacg acgcgcttct
    27721 tcttcggctt tacgcttgag ctctgcagct tcctgctcac ggcgggcttt ttcagcctgg
    27781 gcgtttttag tcatatcgtc ttgttgattg ctcactttgt ctttttccgc agcttcacgt
    27841 ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg cctcacgttc agctttttgt
    27901 tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg cttgctcttc cgcttcacgc
    27961 tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg gatcgcgttt cacaaaggtg
    28021 cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc caccggtacc aggaatgtta
    28081 agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg ggcctgaatt tttctgattc
    28141 aggtggtcaa tcaaagtctg tttctcttgt gcagacacag agtcgtcagc agacttccgg
    28201 atacctgcat cagcaaattg ctgtaccagg cgttccacgg aggtctgtcg ctctgcggcc
    28261 agcgttttaa tcgttacatc tgtcatgctg ttccttcctg ctacagttta ttacgcttcg
    28321 tcaccgaacc agcaaatatt acgggcagcc ataatcagtg ctccggcttt ttcgtcggtc
    28381 aacccttcga tatcagccag atcatcaatg ccctgttcgg cgagatcttc cagcgtacaa
    28441 acgccacggg cggccagttt gaatgccaaa tcacgatcta ccccttcaag gttcagcaga
    28501 tcgtcagccg gtttgttatc accgaggctt tcttcctgga cctgtgcaat ggtggccagt
    28561 gcatttttag cacgctcgcg cagtgcttca acggtcggct catcaaggcc ttcgatttcc
    28621 aacagctctt tcatcggcac ataggccagt tcttccagcg tcgagaagcc ttcctctacc
    28681 agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg tgaaggtgtc gatcgctgcg
    28741 tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg tcatcacgtt gagttcccaa
    28801 ccgctcagct gcgaagccag acgcacgttc tgaccgttac ggccaatcgc ctgcgccagg
    28861 ttaccggctt caacggcgat atccatggtg tgtttatctt catccaccac gatagaagca
    28921 acgtctgccg gtgccattgc gttaatcaca aactgcgccg ggttatcatc ccacaggacg
    28981 atatcgatac gctcgccgcc cagttcagta gaaaccgcct gaacacgcgc gccacgcata
    29041 cctacgcaag cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc gattttcgca
    29101 cgagaacccg ggtcgcgagc cgctgcttta atttcaatca cttcttcgcc gatttctggc
    29161 acttcaatac ggaacagttc gatcagcatt tccggcttgg aacgagtgac gaacagttgc
    29221 gcgccacgcg cttccgggcg aacggaataa agcacgccac gaacgcggtc gccagggcgg
    29281 aagttttcac gcggcagcat atcttcgcgc aggatcacgg cttcagcgtt gttgcccaga
    29341 tccagagaga tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat ttcaccttcg
    29401 tgttcacgga actgatcaac caccatcgca cgttcggctt cacgcacttt ctgcacgata
    29461 acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa cagactcaat ctgatcttca
    29521 acgtaatcgc ccaggttcag gctttcatct tcataacgtg cggcttcaag ggtgatttcc
    29581 ttggtcggct gggtgacttc atcaacaact aaccagcgac ggaaggtgtc aaaatcaccg
    29641 cttttgcgat cgatctgtac gcggacgtcg atctcttgtt catatttttt ctttgttgct
    29701 gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc cttttcattg
    29761 gatacggctt caactacagc caaaatttct ttgttcatcg cgggcttttc acctcatcca
    29821 gactattaaa agtggggaac caagttcgcc ttctggatat tactcagcgc gaacacttca
    29881 tctttacctt cgacggtaac tgtgatcatt tcaccgtcta ccgctttgat aacgccctgc
    29941 catttacgac ggttttgtac cgccatacgg agaaccagag tcacctcttc tccgacaaaa
    30001 cgggcgtagt gttcagccgt gaacagtggg cgatcgagac ccggtgagga gacttccagg
    30061 ttataagcaa cggtgatggg atcttcaaca tccagcacag cacttacctg gtggctcaca
    30121 tcagcacaat catcaacatt gatgccatct tcactatcaa tatagatgcg cagtgtggat
    30181 gtgcgaccgc gaataaattc gatgccaacc agttcaaaac ccagggcctc aactggcgca
    30241 gtaatcatct ctgttaattt ttgctctaat gtggacaagc ccacccccaa gacataaaaa
    30301 aagggcctaa agcccagtta ttctgtagtc agataacaaa aaaccccgat aaatcggggc
    30361 tttatataac tgaaccctat aaccgcaact gcggtctgga gcactttcca gaaggatttt
    30421 ttcaaatccc actacgaagg ccgaagtctt cacagtatat ttgaaaaagg actctaaggg
    30481 aaagtggttg cgggggccgg atttgaaccg acgaccttcg ggttatgagc ccgacgagct
    30541 accaggctgc tccaccccgc gcctgaaacg tggcaaattc tactcgtttt gggtaaaaaa
    30601 tgcaaatact gctgggattt ggtgtaccga gacgggacgt aaaatctgca ggcattatag
    30661 tgatccacgc cacattttgt caacgtttat tgctaatcac gtgaatgaat atccagttca
    30721 ctttcatttg ttgaatactt ttgccttctc ctgctctccc ttaagcgcat tattttacaa
    30781 aaaacacact aaactcttcc tgtctccgat aaaagatgat taaatgaaaa ctcatttatt
    30841 ttgcataaaa attcagtgag agcggaaatc caggctcatc atcagttaat taagcagggt
    30901 gttattttat gacgacgatt ctcaagcatc tcccggtagg tcaacgtatt ggtatcgctt
    30961 tttccggcgg tctggacacc agtgccgcac tgctgtggat gcgacaaaag ggagcggttc
    31021 cttatgcata tactgcaaac ctgggccagc cagacgaaga ggattatgat gcgatccctc
    31081 gtcgtgccat ggaatacggc gcggagaacg cacgtctgat cgactgccgc aaacaactgg
    31141 tggccgaagg tattgccgct attcagtgtg gcgcatttca taacaccacc ggcggcctga
    31201 cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg taccatgctg gttgctgcga
    31261 tgaaagaaga tggcgtgaat atctggggtg acggtagcac ctataaagga aacgatatcg
    31321 aacgtttcta tcgttatggt ctgctgacca atgctgaact gcagatttac aaaccgtggc
    31381 ttgatactga ctttattgat gaactgggcg gtcgtcatga aatgtctgaa tttatgattg
    31441 cctgcggttt cgactacaaa atgtctgtcg aaaaagctta ctccacggac tccaacatgc
    31501 ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa ctccagcgtc aaaatcgtca
    31561 acccgattat gggcgtgaaa ttctgggatg agagcgtgaa gatcccggca gaagaagtca
    31621 cagtacgctt tgagcaaggt catccggtgg cgttgaacgg taaaaccttt agcgacgacg
    31681 tagaaatgat gctggaagct aaccgcatcg gcggtcgtca cggcctgggc atgagcgacc
    31741 agattgaaaa ccgtatcatc gaagcgaaaa gccgtggtat ttacgaagct ccggggatgg
    31801 cactgctgca cattgcgtat gaacgcctgt tgaccggtat tcacaacgaa gacaccattg
    31861 agcagtatca cgcgcatggc cgtcagttgg gccgtctgct gtaccagggg cgttggtttg
    31921 attcccaggc gctgatgctg cgtgactctc tgcaacgctg ggttgccagc cagatcactg
    31981 gtgaagttac cctggagctg cgccgtggga acgattattc aatcctgaat accgtctcag
    32041 agaacctgac ctacaagcca gagcgtctga cgatggaaaa aggcgactcg gtgttctcgc
    32101 cagatgatcg tatcggtcaa ctgaccatgc gcaacctgga tatcaccgat acccgcgaga
    32161 aacttttcgg ttatgccaaa actggcctgc tttcctcctc tgccgcttca ggcgtgccgc
    32221 agatggagaa tctggaaaac aaaggccagt aattcgacat caaccctgcc cttcaggggt
    32281 ggggttacct tgcttatcct ctgtaaactc ctttctgagc atcacataat cgaaaacatc
    32341 atggtttgca tccacaacca gtgagtctcc tttatagtgt acaatcactt cctcaagcgt
    32401 ttgcgactgt tccggctttg ttacccccat ccacgcatta ataaggtaat tattgtaagc
    32461 tgtcgaaaag atgttgtttt ccgttgtgcg atccacacca tccccaagaa cggggctata
    32521 ccagataaac atcggaacat gatatgcctg ctggctggcc tccctgcctc catgaaaata
    32581 gacgttcttc ttcgtagggt cacgttccag gccgtggtcg gcaaaataca tgaccgaggc
    32641 gcggcgatct tttaataatt caaaaacctg acctagcaaa ctatctgtgt aatgaatgga
    32701 gttgtcatag caggcatcct gatcgtcctg cggttgaaac acggcgctgg attgcggata
    32761 ggcgctacaa gccggttcat ggcttccatt taaatgaaga acaatcagtt tcttttgctg
    32821 cgtattttgc tgtaatgctt gcgataaatg cggcaacaac aattcatcaa atcctctgac
    32881 ataaactgtt tccatggcac gcatggcgat actggtaact gctgtaccat tctgccgaaa
    32941 agcggattgc gagcttagcc agaaagtctg aaatcctgct tgattagcca tattaataat
    33001 gttgtcgggg taattatgaa tgtcatggct caaaacagaa tcagcagtta aagaaagggg
    33061 aaccgacagc gcagtgtaag gtgcaccgct tattgcttga ttaaacagtt tgatctgttt
    33121 tctttgtgct tcgacttgcg gtgtcgtaga gcgtgtatat ccatacaaag acatattgtc
    33181 gacacgtaca gactccccca caatcaacac gtaggtatca atacctgtat ccctgactga
    33241 taattgaaaa tacggcactg tgtttgcaat tgagagtaat ctttgatgct ctttcgctgc
    33301 taaagcaaaa taattgagat tgaaaaacgg cgtataggta gcaaatcgcg acgctagtat
    33361 atatggactg aacgcatttt tatttttggc atctttataa gcaaattgac aagcggaaaa
    33421 taaacttccc gaaatgacaa tcagcaataa tattcctgtc actttttttg tcggcaagga
    33481 aacatcatat tttattatta cagccaaaaa aagaagggat aaaaaggcaa aagcacatag
    33541 ataagggcta tacattccca gcattttggc aacttcatcc ggatcactct gcagcacact
    33601 aatcgcaaat ccatcattga aagttgtacc aaaggtacac catgaataga ggctaatact
    33661 catgtctgcc gcagtcagta acacaaatgg tgcggcaaca agcaggcgaa gcggaaggga
    33721 tgatatacgt ttaacaacca ggatggtcag aacgaagaac agcaatcgct gaaccatatg
    33781 cgctccggaa gaggcgacta agttcgtaat accaaaaaca ataacactaa gatacaacaa
    33841 ccaatgagat ttaaaacttc tagcaaattt gttgaatact gtcatccaga aatccaagtt
    33901 cacaaccaat ataaacaatt atcaacaatg tgcaatttta tatcagacat gtttttcaac
    33961 tctgatccat atcagaacga aacactgtta gttttttctt tttaaatgat gcggccattg
    34021 cgttctcagt aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc
    34081 atcagtagtt atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc
    34141 gtaccggaac tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt
    34201 ataacgaagg cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga
    34261 cgagatacag atacctgaca accattcctc agaccaggac caaaacgaaa aaagacgctt
    34321 ttcagcgtct cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat
    34381 tgggcactac cacctcaagg tagcgtgtct accaattcca ccacctcggc acggaaacta
    34441 ctttttagtt cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg
    34501 ttttcgccgg cgcactcagg ttttcccatt cgctaccttt attggtcttg ttgctattga
    34561 tgttacccag caccagactg atgatgaaga ataacgttgc cagcaacgcc gtcatgcggg
    34621 tcatgaagtt accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg
    34681 aggctcccat atcagcgcct ttaccttgct gtagcatgat cagaccaaca aggccaattg
    34741 ccacaataag gaaaactacc aaaagagctt cgtacataat caacctgttc cttgcggagt
    34801 tgccgcgtac caatgcttca accaataaag cgggagttta taatctttcc cactgaagcg
    34861 ggtgtgaata ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca
    34921 actgcggaaa aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc
    34981 ctttttagcc agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc
    35041 aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt
    35101 gccggattta cgcaacagca cacgtccacg gctacccagc gccgtttcaa cttctgcggt
    35161 caccgctttg actgattcat gctccagcgg atcgccgcta cctgcggtgt aacgcacgtt
    35221 taccaggatt tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt
    35281 acgcgccatc gccgccagca cctgcaaacc agcaacgatg ccgtcaccgg tagtggtttt
    35341 atccagcagg atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat
    35401 tttttccagt acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt
    35461 cagcgccagt tcaagcccca tgttgctcat caatgtaccc acagcgccac cacgcagctg
    35521 gccctgacga agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt
    35581 gccttcatgg tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc
    35641 cgctttttca gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc
    35701 attgatgttt acaccgtttg gctcacaacc gatagcgata acattcgctc ccagttcgcg
    35761 cagcacgttc ggcgcgatgt gataagtcgc accgtttgcg caatccacca caatcttcag
    35821 ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc
    35881 agcatcaacg atacggctgg ctttacccag ttctgccgaa tcaacacagc tgatctcttt
    35941 ttccatttcc gcttcgatgg cttcttcaac tgcatccggc agcttagtac cgtcaataga
    36001 aaagaattta atgccattat cgtagaacgg gttatgcgat gcagatatca caattccggc
    36061 ctctgcgcgg aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa
    36121 gagtgcggaa aggcccgcag ccgccagccc cgcttccagt gctgactcca gcatatagcc
    36181 agaaatacgc gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt
    36241 acccgcggcc caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg
    36301 accacgaatc ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg
    36361 cagacagagt agcttccacc actcgcatcg cttctacggt ttctttgacg tcatgaacac
    36421 gaataatgtg cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc
    36481 gctcggacgg ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca
    36541 acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg
    36601 agagattttt accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg
    36661 cctgctcgca acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt
    36721 catacttcgg ggcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg
    36781 gtaaaccggt ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcgt
    36841 taataatgtg agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga
    36901 ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca
    36961 actcttcttc aacgctaact tccgccgccc ctggccgcgt ggactcgcca ccaacgtcaa
    37021 tgatcgtcgc gccagcgttg atcatcagat tcgcatgttt caccgcatct atcagcgagt
    37081 tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag
    37141 gatggctaag gtccagtgaa gtaccctggg caaagagttt catgatgtta tccctggtat
    37201 gaattgataa gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc
    37261 tgatgcggga acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt
    37321 acgcggttca tcaaccggac gaggagcctt tggactacca ttgtcgccag cattgttaga
    37381 agcgcctggt tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc
    37441 aatctgcggt gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat
    37501 atccatattg tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt
    37561 cacttcctgg tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct
    37621 acggccgagg aacacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga
    37681 gaagccccac tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga
    37741 cgcaccggta gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta
    37801 cagcgtagaa atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg
    37861 caagaagaaa gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tgtgcaccgg
    37921 atcgtgctcc ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgtcga
    37981 ttctttctgc gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc
    38041 tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag
    38101 tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgggc
    38161 aatgattgcc gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ccttcaggat
    38221 ctgctcacga ccgcgaacat ctggcaagcc aaccacaacc tgacggtcga aacggccagg
    38281 acgcagcagc gctgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc
    38341 ttcgttacct tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc
    38401 atcgtgacca ccgcccagac ctgcgccacg ctggcggcct acggcgtcga tttcatcgat
    38461 aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc
    38521 acccacaccg acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt
    38581 cgcttcgcct gcaatcgctt tcgccagcag cgttttaccg gtacccggag gaccgaccat
    38641 caggacgcct ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata
    38701 ctcaaccagt tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt
    38761 cgttttgatc tgatcttccg tcagcatgcg cgctttactc ttaccaaacg acatggcacc
    38821 tttgccaccg ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag
    38881 catcgggaac caggagatga agatagaagc cagcaggctg ggttcttcag gcggttcacc
    38941 gacaaccttg acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat
    39001 gtaagtggta taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg
    39061 cgcttcacga acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg
    39121 gccattagac tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac
    39181 cagccagagt attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg
    39241 tgttaaaaac agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc
    39301 tcccggttag ggtttacgcc cggtcgctac aatatacact tcccgcgaac gtgcacgaga
    39361 agagtccggc ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc
    39421 atcgaaacct tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc
    39481 acgacacatt tctagcgcca gttccaccag atacatggca cgggggatat ccaccgccgg
    39541 tgttccgctc atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac
    39601 gcgctccagc agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac
    39661 accaacgata ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat
    39721 ttgggtgacc acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat
    39781 tcccggttta aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga
    39841 acgtaacccc tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca
    39901 gcggctggag ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc
    39961 atcgtaacca attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt
    40021 agaatgaccc gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac
    40081 aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta
    40141 atggtttgac cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca
    40201 tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac cttgatcgtg gaagctatcg
    40261 tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc
    40321 caactaaaga acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt
    40381 gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt
    40441 gataaaaggc cgctatgcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat
    40501 tgggtaattc ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg
    40561 ttttgatgac cacaacatca tcttcttctt tgccgatcag gccacgagca ataggtgagt
    40621 ttacagaaat caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct
    40681 gttcttcgtc agaatcgaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat
    40741 tgttgggcat tttggtgaca tcaatcacct gcgcgttcga cagcttggct tcgatgtctt
    40801 tgatacggcc ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt
    40861 tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag
    40921 atttcagaaa atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag
    40981 cttgcatttg ttatacctct tgaatattcc tgatggggca agtgttcacc caccccggct
    41041 gttcggcctg cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg
    41101 tacaagtccc aggtcagcta caattcacat tttgatagtc attttaccct gaagttcccg
    41161 aagggtcatc gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt
    41221 gttagcgcga gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt
    41281 tcagtgttca ggccgcaaat gttgatgagt acattactca actccccgct ggtgccaacc
    41341 ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc tattgattac cacagtcagc
    41401 agatggcgct gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac
    41461 tcggccccga ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg
    41521 tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaagata
    41581 ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt
    41641 tgatagatac ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca
    41701 tgacacaatg ctttagcgct ccgcctgccg tcgccatagt tgaccgcaac tgtttctccg
    41761 tctcgctcta cagtgcccca aaacctggtg atatggcttt tatacgcgtg gcatcttatt
    41821 accccgttac gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat
    41881 actgcgaact ggatgtggtg ccaggcgacc tgaaccgctt tacgctgacg ggatgcctgc
    41941 cacaacgttc tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg
    42001 gtgcaattct gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc
    42061 gccagactca ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc
    42121 acgatctgct taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc
    42181 gcatgatagg ccatgcgcgc ttcaatgtgc ctggaacatg gcgggccggg tcggacgctg
    42241 tgcgtcagat cctgcgccag caagccggtg tcgatattgg aaacaccatt attgccgatg
    42301 gttcagggct ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat
    42361 acattgccca acacgacaat gaacttaact ttatctccat gctgccgcta gcgggctatg
    42421 acggctcttt gcagtaccgt gcaggtctgc atcaggcggg cgtggatgga aaagtctcag
    42481 cgaaaaccgg ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg
    42541 ggcaacgaat ggcgtttgta caatatcttt ctggctatgc agtagaaccg gcggatcagc
    42601 gtaatcgccg tattccgtta gtgcgttttg aaagccgttt gtataaagat atttatcaga
    42661 acaattagtc aaaaagaaac cccggcacat ggccggggtt ttgctgatta acgcttgtaa
    42721 atgaactcaa cgccttcttc gtcgtcttcg tcccagtcgt catcccagtc ctcatcatcc
    42781 tcttcagcaa tctcttcaag ctgctggcga tgataatcat cccacatgaa ttcgactttc
    42841 tctggctgtt tcgcttcttc agcctgcacg accgggtttt caatgataaa ggtcatcaca
    42901 tcccagcaga gatctttcac gcccagtcca ctcgccgcag agatcagata atatttatct
    42961 tcccagccca gcgcctctgc gatcgctttc gctttctctt cggcttctac cttatccagc
    43021 agatcgatct tgttgaagac taaccaacgc ggtttcgccg ccagatcctg gctgtatttt
    43081 tccagctcgc tgataataat acgcgcgttt tcaaccggat cggtgccgtc aatcggatcg
    43141 atatcgatga ggtgcaacag gacgcggcaa cgttccaggt gcttcaggaa gcgaatgccc
    43201 agacctgcgc cttccgcagc gccttcaatc agtcctggaa tatcggcaac aacgaagctc
    43261 ttttcgttgt ccattcgtac cacacccaga cttggcacca gagtggtaaa cggataatcc
    43321 gccactttcg gtttagccgc cgataccgca cgaataaagg tcgatttacc cgcgtttggc
    43381 atccccaaca taccgacgtc agccagcagc atcagctcca gcagcagctc acgcttatcg
    43441 cccggcgtac cgttagtttt ctgccgtggt gtacggttaa ctgacgattt gaaacgggta
    43501 ttgcccagac cgtgccagcc gcccttagca accagcagac gctgaccgtg tttggtcata
    43561 tcgcccatgg tttcaccagt accctggtcg attacacgcg tacctaccgg cactttaatt
    43621 gtgacgtctt taccgcgctt accggtacag tcgcggcttg cgccattctg accgcgctct
    43681 gcacggaaag atttttcaaa acgataatcg ataagcgtgt tcaggttctc gtcggcttcc
    43741 atccatacgt caccaccatc accgccgtcg ccgccatccg ggccgccttt cggaatatac
    43801 ttttcgcggc ggaagctcac gcaaccatta ccgccatcac ctgcaacgac cagaatcgat
    43861 gcttcatcaa caaacttcat tttattctcc gtaaatcatt cgcctgcgcg gggttgcgat
    43921 accaccgttg tatgcttacg taatccgccc caaatacgat gaccaatggc ggaatacatc
    43981 gcgcccgcaa ccacgacaaa cgcaccgaga taacctaaaa ggtttaacat cggtctggcg
    44041 aagaaatcgg gccaggccag tgataaaaga tctgaaaaaa acagcgtaaa cagtggggtg
    44101 agcgtgatga tcgcgctcac ctgcgctgcc tgccagcgag ccatcgcttc cgccagggcg
    44161 ccatatccta ccaaggtatt cagtccgcaa aaaattaaac atgcgagctg ccagtggcta
    44221 agctgcgcta tcactccagg ctttgccaga gggaagagcg caattgtaca taaagtgtac
    44281 agtaaaaaca ggatctgcgg tgaggccagc cgacgcaata aaaccttttg cgccacgcca
    44341 taactcaccc aaaccgtcgc agcaccgacc ccaaagataa ctccccaggt gtaatcggtt
    44401 agctttgtaa atatctcgac cagactggtg ttaaaaaaca tcaccaggcc gctcaggagc
    44461 atcaatgccc ctacaacctg agtgctgcgc attttctctt tcaggataaa tacgctggca
    44521 accatcatgc caactggcga gagttgccca atcacctgcg aagcggtcgg actcagggat
    44581 ttcaaggatg agctgaacag gatgaagttc ccaaacagcc cggcggtcgc cactgccaac
    44641 aaaatcaacc agcgtggctt acgaaacacg cgtaatggcg gcaacctctt cttcaccgca
    44701 agaatggccc ccaggccaat actcgccatc aagaaacggt aaaacacgat tgtcggaggt
    44761 tccatcacct ccagcacctg cttcattgca attggcaacg ccccccagca aattgctgtg
    44821 gtgagcgcca aaagaatgcc aatgcctgcc tgctgcttca taccgtattc cctgcaaagc
    44881 gcatttgcgg attaacgtcg caccatgcgc atttttaccg gttatcgaat gtaaaaagcc
    44941 ccgcaacgtg ttgcggggct ttcatccgtt accgggacgc gaaaaactta ttcagcttcg
    45001 atgctgataa atttacggtt tttcgggcct ttaacttcga atttcacttt accgtctgct
    45061 ttagcaaaca gagtgtggtc acgaccgcaa cctacgttag cgccagcgtg gaatttggta
    45121 ccacgttgac gaacgatgat gctacccgcc agaacggatt cgccaccgaa acgcttaacg
    45181 cccaggcgtt tagcttctga atcgcgaccg ttacgtgtgg agccgccagc ctttttatgt
    45241 gccatttgaa atctctcctc aggtcttagg cgctgatgcc agtaattttc acatcagtga
    45301 accactgacg atggccctgc tgcttacgat agtgtttacg acgacgaaac ttaacgattt
    45361 taactttctc gccacgaccg tgagcaacaa cttcagcttt gattacgccg ccatcaacga
    45421 aaggaacgcc gattttgact tcttcaccgt ttgcgatcat cagcacttca gcgaactcaa
    45481 cagtttcgcc agttgcgatg tccagctttt ccaggcgaac ggtctgacct tcgcttactc
    45541 ggtgttgttt accaccactt tggaaaaccg cgtacatata aaactccgct tccgcgcaca
    45601 ccttttcaat gattcagagt gcgctataaa tattcacaat agggcgcgaa tattacgcaa
    45661 aacgcacgcc tttgacaagt gctacagtca atacacgaag aaaaaaaaca caacttgtac
    45721 ggtaacgttt atctgtgcca ttttttcagt acaatcaccc tatattccta accataaacc
    45781 ctaagttgcc tttgttcaca gtaaggtaat cggggcgaaa agcccggctt ttgcgatgaa
    45841 tttagaaaaa atcaatgagt taaccgcgca agatatggcg ggtgttaatg cggcaatcct
    45901 tgagcagctt aattccgacg tccaactgat caatcagtta ggctattaca tcgtcagcgg
    45961 cggcggtaaa cgtattcgtc cgatgattgc tgtactggct gcacgagctg ttggctatga
    46021 gggaaatgcg catgtcacca ttgctgccct gatcgagttt atccacacgg cgactctgct
    46081 gcacgacgac gttgtggatg aatcagatat gcgcaggggt aaagctaccg ccaacgccgc
    46141 atttggcaat gccgccagcg tgctggtagg cgattttatt tatacccgcg cgttccagat
    46201 gatgaccagc ctcggttcac tcaaagtgct ggaagtcatg tcagaagccg taaacgtcat
    46261 cgcagaaggt gaagttctgc aactgatgaa tgttaacgat ccggacatca ctgaagaaaa
    46321 ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt gaggctgccg cgcagtgttc
    46381 cggaattctg gcgggctgta cgccggagga ggagaaaggc ctgcaggatt atgggcgcta
    46441 tctcggcact gctttccagt tgatcgacga tttactcgat tacaatgccg atggcgaaca
    46501 gttaggtaaa aacgtcggcg acgatctgaa cgaaggtaaa ccgacgctgc cgctgctgca
    46561 tgcgatgcat catggcacac cagaacaggc acagatgatc cgtaccgcca tcgaacaggg
    46621 taacggtcgc catcttctga aaccggttct ggaagcaatg aacgcttgtg gatctcttga
    46681 atggacgcgt cagcgcgccg aggaagaagc agacaaagcc atcgcagcgt tacaggtgct
    46741 cccggacacc ccttggcgag aagcactcat cggcctcgcg cacatcgctg ttcaacgcga
    46801 tcgttaatcc cctcccctca tcccgcgcag tgcgcgggat gattccagta aactccataa
    46861 gaatacctat tcagcgtaat aatgcactga aaaaccagtg catacatctt tatatattct
    46921 gaatattcac actctttata ggaacttttt agagcaatcg gccatcagga gtatagtgat
    46981 gctcgacaga agaagtgttc tgaatgaaag cgactaactt aaggagtgag gaaagatgga
    47041 aagtaatttc attgactggc atcccgctga catcattgcg ggtttgcgca agaagggaac
    47101 atcaatggcg gcggagtctc gcagaaatgg tttgagttcc tcaacgctgg cgaatgcatt
    47161 atcgcgccca tggccgaaag gagagatgat tattgcgaaa gccctgggaa ctgacccctg
    47221 ggttatctgg ccatcacgct accatgatcc gcagacccat gagtttatcg acagaacgca
    47281 gttgatgcgt agctacacta aaccgaaaaa atgagtggtc tggcggtagc cccgcgaacg
    47341 gggctgccag ctctcagacg attattcgcc tttcacacgc tcaatatttg cacctaaagc
    47401 gcgcagtttg tcttcaatgc gttcgtatcc acgatcgatg tgataaatac gatcaaccac
    47461 cgtcgtccct tccgcaatac agccagccag caccaggctt gctgatgcac gcagatcggt
    47521 tgccataacc tgtgcgccag aaagtttttc aacaccgtga caaataacgg tattgctttc
    47581 gatttcggcg tgcgcgccca tacggctcag ctctggcaca tgcataaagc ggttttcaaa
    47641 gaccgtttcg gtgatgaatc cagtcccttc tgccaccagg ttcaacagcg tgaactgggc
    47701 ctgcatatcg gtcgggaatg ccggatgcgg cgcggtacgt acgttaacag ccttcggacg
    47761 tttgccatgc atatccaggc taatccagtc ttcgccgacc tcgatgtccg ctccagcgtc
    47821 acgcagtttc gccagcacgg cgtcgagagt atctggctgc gtgttacggc agataatttt
    47881 gccgcgagaa atcgccgccg ccaccaggaa agtaccggtt tcgatacgat ccggtagcac
    47941 gcgatagaca ccgccgccta aacgttccac accttcgatg acgatacgat cggtgccctg
    48001 accgctaatt ttcgcaccca gcgtaatcag gaagttcgcg gtatcgacga tttccggttc
    48061 acgcgctgcg ttttcaataa tcgtggtgcc ttccgccagg gttgcagcac acatgatggt
    48121 caccgttgcg ccaacgctga ctttatccat cacgatatgt gcgcctttca gacgaccatc
    48181 gacggaagct ttaacgtaac cttcttccag tttgatggtc gcgcctaatt gttcgaggcc
    48241 agaaatgtgt agatcaaccg gacgcgcacc gatcgtacaa ccgccaggta gtgaaacttg
    48301 cccctgacca aagcgcgcta ccagcggccc cagtgcccag atagaagcac gcatggtttt
    48361 aaccagatcg taaggtgcgc agaatacatt aacgtcgcgg gcatcaatat gcacagaacc
    48421 attacgttct actttcgcac ccagctggct tagcagcttc attgatgtat cgacgtcttt
    48481 cagtttcggg acgttctgga tctctaccgg ttcttccgcc agtagagcgg caaaaaggat
    48541 aggcagagca gcatttttag cgccggaaat tgtgacttcg ccctggagct tcgttggccc
    48601 ctgaacacga aatttatcca tttagtttgt tctcagttaa caattcatat ccgctaccgg
    48661 cgaatcgccc atagctcaaa agccgttcag tttgcgatcg cgcgcccact ccgcaggggt
    48721 atacgctttg atcgacacag catgaatgcg gttatccgca atatattcca tcagcggacc
    48781 atagaccgtc tgctgttttt taacccgact catgccgtca aacaactcac ccacggcaat
    48841 aacctgaaag tggctgccat cgccggaaac gtggacttcc tggagggaga gagcgttcat
    48901 caacacgctc tgaatttcat tattttccat gggatcttca atcatcagtt aataaaccag
    48961 cgaaacatct tagagcaaag ttgcgctggc ataaataagc aaaaagcctc gctgataaat
    49021 cagacaaggc tcgacttgca ggcaggtttg ccggacaggc ggttaacgcc atatccggcc
    49081 tgaaaaaatt taacgaggca gaacatcagc aggcaaatta tacaatttcg ccagggtata
    49141 cactttgtcg tttaccccct gaagcgtcac gttgttgccc tgctttttcg ccagatcgat
    49201 aagatggagc agcagtgcca gtccacccgt atccacgcgg gacacacggc taagatcgat
    49261 gcaggtaatc cccttcaccg cttcctcacg catttcccaa agcggtagca gaacgtcctg
    49321 atccagctct ccggataacg ccagtgtgtc acccgtctgc atccagctca gtgactcgct
    49381 cattattttt tctcttccag agtgattttc tgttgagaaa tcgatttcag ttgcgcagtc
    49441 aggccgtcga tacctttggt acgtagcagc gttccccact cgttttgttt ggtggtgatc
    49501 atactgacgc cttcagcaat catgtcgtaa gcctgccagt tgcccgtctg ggagttttta
    49561 cgccactgga agtccagacg caccggcggg cggccattcg ggtcaataat ggtaacgcga
    49621 ataggcacaa tggttttatc acccagcggc tgttctggcg caatctgata ggtttgaccg
    49681 tgatacatcg ccagcgcctg accgtaagcc tgcttcaggt actcacggaa agcggcaaag
    49741 taagcatcac gttgagctgg ggtcgcactc ttgtaatact ggcccagcac cagcgcaccg
    49801 gcgtatttca cctgtacgta tggcagcagt tcctgatcaa caatggtacg cagataatcc
    49861 gggttggccc gaatttgcgg ttgctcattc ttcaggcgat cgaacgtttt ctgcgccgcc
    49921 tcgtccatca gcttatacgg attggtctgg tctgccgcgg ttgccgcact cagcggtgca
    49981 atcaccagca aagcgaccat cattaaacgt ttaaacatgc gtcggttctc ctgaaattat
    50041 ttcgttgtac ccacaggttc agtggtttca ttattacctg gcgcagcagc tggcgcatcg
    50101 ccactattct tattgtcatc gcctttacta ccgtaaagga actgaccaat gagatcttcc
    50161 agcaccatcg cagacttggt gtcctgaatt gtatcgccat ccttcaggat agcagtcccc
    50221 agttccgggt cttcaaaacc gacgtttaat gccagatatt gttcccccag caggccggaa
    50281 gtacgaatgc tcagcgaact ggtatcagga atatggttat aacgttgttc aatttccagc
    50341 gttacgcgcg gcagataggt tttcgggtcc agcgtaatat ccgccacccg gcccacaaca
    50401 acgccaccaa tactgaccgg agagcgcgct ttcaggccgc caatgttatc gaacgtcgca
    50461 taaagcgtgt aggtcggttc agtacgtatt gacgtcacgt tcgccgcctt caggcaaaca
    50521 aacagcgccg ccagcagtgc tgctaataaa aagataccca cccaaatttc attttttttc
    50581 gtttgcatga actcaattcc caaacatcaa tgcggtcagc acaaaatcca gccccagaac
    50641 agccagagac gagtggacaa cggtgcgagt ggttgcccgg ctaatcccgg cagacgtcgg
    50701 gatggcgtcg tagccgttaa acaacgaaat ccacgtcacc gtgatggcga acaccacgct
    50761 cttaatcaga cagttgacca gatccatacg ccagtcgacg gcattttgca ttgccgacca
    50821 gaagaacccg ctatcaatgc ctttccaact gacgccgacc agcgatccgc cccagatccc
    50881 cacggcgacg aagataaccg tcaacagtgg taatgaaata accccagccc agaaacgggg
    50941 agaaataacc cgacgcagcg gatccaccgc catcatctcc atactggaga gttgctctgt
    51001 agcgcgcatc aggccgattt ctgcggttag cgccgaacca gcacgcccgg caaacaacaa
    51061 cgcggcaacc accggcccca gttcacgcag tagcgataac gccaccagca tacccagact
    51121 ggtttccgca ctataagtgg tcagaaccag ataaccttgc agccccaaca ccattccgat
    51181 gaacacgcca gaaactacaa taatcagcat cgacaggacg ccgacattat agagctggcg
    51241 caccagcagc ggcgcatgtt tgcgaaattc cggtttgccg accagcgcat tgaataacat
    51301 taacccggcc cgcccaaacg ttctcagggt tttaatccct ttatgtccga gcgacgccag
    51361 cgcatttaac agcatgagtg gcttaactcc ctggtaaaag atcagcgtga taatcgccgg
    51421 caggatagcg gaacggaacc ggcccgtcag ctatcccgtc cagaaactga cgtacgcgcg
    51481 gatcaggatt cgcctgcaac gcctgggcac tgccatgagc gacaattttt ttgtccgcca
    51541 ggatccaggc gtgatccgca atacttaaca cttccggcac atcgtgagaa accaccacac
    51601 aagtcacgcc cagcgcgctg ttcagctcag aaatcagctt caccagtacg cccatggtga
    51661 tgggatcttg cccaacaaaa ggttcatcaa acatgatgag atccggctcc agcgcaatcg
    51721 cacgcgccag cgctgcacgc cgcgccatcc caccggaaag ttcagaaggc attagtttag
    51781 ccgctccacg cagccccacg gcctccagct tcatcatcac cgtactatgc aacaatggcg
    51841 cgggaagttg ggtatgttcg cgcagtggat aggcgacgtt gtcaaatacg ttcatatcag
    51901 tgaacaacgc cccggactga aataacatgc tcatccgttt gcgcactgta tacaggcgcg
    51961 aacgagacat cgccggaata ttctcaccat cgaaaaggat ctcaccatga tctggtgcga
    52021 tttgcccgcc aatcagacgg agtagcgtcg ttttaccgat gcccgatggc cccatgatcg
    52081 ccgtgatctt ccctcgcggc acggtcaggg aaatattatc gaagatgcag cgattgccac
    52141 gcgtaaaact gacatcgcgc atatcgacta aattcgccac agactgctcc ataattcacc
    52201 cttcgtcttg cgttgatttt ctaagcatgg cgctcaattt aaccttgaac ccaacatatt
    52261 tacagaatat tacccgccgt ggttagcgaa agctggcatt tgttttactt tttagccgca
    52321 taaagtcaaa attaagaatc cgttacggct ttctgaaaat cttcagcgga ccggcgagta
    52381 tacctgaaga aaggacgtta gatgctttta gctacggcac tgttaattgt tggtttactt
    52441 ttggtcgttt acagtgccga ccgcctggtt tttgccgcgt ctattctttg ccgaaccttt
    52501 ggcatcccgc cgctgatcat cggcatgacg gtggtcagta ttggtacatc gttaccagaa
    52561 atcatcgtct cgcttgccgc gtctctgcac gaacaacgcg atttagccgt tggtacagcc
    52621 ctcggctcaa acattatcaa tatattgctg atcctcggtc tggctgcgct ggttcgtcct
    52681 tttaccgtcc attccgatgt tctacgccgt gaattaccct taatgttgtt ggtcagcgtg
    52741 gtggccggtt ccgtactcta tgacggacaa cttagtcgca gcgatggtat ctttctcctc
    52801 tttctggctg tgctatggct gctgttcatt gttaaacttg cacgtcaggc tgaacgtcag
    52861 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    52921 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    52981 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatgggtctg
    53041 acggcaattg ctatcggaac cagcctgccg gaactggcaa ccgcaatagc gggcgttcgc
    53101 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    53161 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    53221 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    53281 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    53341 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    53401 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    53461 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    53521 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    53581 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    53641 tggtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    53701 ctctaactca ggtgaatcca gcgaaatcac tgccttaatt ccagtgctta agcgtcttca
    53761 cataccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    53821 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    53881 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    53941 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    54001 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    54061 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    54121 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    54181 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    54241 cgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    54301 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    54361 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    54421 cttgcgacct gttacggccc tgtcagcgcc gacgttatag caaaagcaga gaacattcgt
    54481 ctgctaatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    54541 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    54601 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    54661 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    54721 gatctgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgtcgg cgatgatctc
    54781 atcgattggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    54841 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcga
    54901 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    54961 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    55021 tcggtattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    55081 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcgctaagct
    55141 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    55201 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    55261 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    55321 ttgtgccgga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    55381 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    55441 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    55501 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    55561 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatacacatt
    55621 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    55681 atcgtcaccc agggcaccat aaaaattaat gccgacaaag tggtcgttac ccgtccgggt
    55741 ggcgaacaag gtaaagaagt gattgacggc tacggtaaac cggcaacgtt ctaccagatg
    55801 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    55861 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    55921 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    55981 cgtgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    56041 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    56101 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    56161 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    56221 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgcta
    56281 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    56341 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    56401 gctgaacagc gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    56461 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    56521 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    56581 atctcagtta tcgacattaa acgcatcatt gagcacctgc gcgacagcgg cctgggcgtg
    56641 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    56701 agtcaggggc atttgatcgc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    56761 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    56821 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    56881 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    56941 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    57001 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    57061 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    57121 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctatc
    57181 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    57241 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gacaccggtt
    57301 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    57361 acgaagttga agccgtcctt aagcggatcc aacggtttga tccggtcggt gtggcggcaa
    57421 aagatctgcg tgactgcctg ctgatccaac tctcccaatt cgataaaacc acgccgtggc
    57481 tggaagaggc cagactgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    57541 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    57601 tccagtcgct cgatccgcgc cccgggcaat cgatccagac tggcgaacct gagtatgtca
    57661 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    57721 gcattcctcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaatg
    57781 atggtgacag ccagtttatc cgcagcaatc tgcaagatgc caaatggttg atcaagagtc
    57841 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    57901 ccttctttga gcaaggcgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    57961 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    58021 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    58081 gcgaagcttc ctccacggcg attcgtgcgc tggtgaagaa attaatcgcg gcggaaaacc
    58141 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    58201 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    58261 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    58321 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagccaaatt tgccaaactt
    58381 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    58441 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    58501 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    58561 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    58621 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    58681 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    58741 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    58801 gaaatcatca gcgagctggc ggcgaaacaa cttagcctac cgcctcaggt ggtttttgaa
    58861 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    58921 catggcaaac tggaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    58981 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    59041 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    59101 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    59161 attacggata ccaaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    59221 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    59281 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    59341 gtagtgttgt tacccgatct ggctcgaacc ctggccgatc gcgagatttc tgccgccgtc
    59401 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    59461 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    59521 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    59581 agtgctatcg acaaagaaag cgatctgctg gagcctctgc gttcgcgagc ggatctgatt
    59641 gtcgatacct cagaaatgtc cgttcacgag ctggcagaaa tgctgcgtac ccgtctgctg
    59701 ggtaaacgtg aacgcgaact gacaatggtc tttgagtctt tcggcttcaa acacggtatc
    59761 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    59821 aaactgcgtc caatgacagg tcttgataag cctgtcgccg cgttcctcga ccgccacaca
    59881 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    59941 gaaaccaaca accgtagcta cttgacggtc gccattggtt gtaccggcgg gaagcaccgt
    60001 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    60061 tcacgccatc gtacgctgga aaaacgtaaa ccatgactgt caagcaaact gttgaaatca
    60121 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    60181 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    60241 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    60301 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    60361 attaatcttc atcactttga catacaactc ccttcaaact ccccccgaca ataagaaaat
    60421 cacgtactga aatcgttctc aatcaacgtc aattgtacat ttcgtgcgct ttttactttc
    60481 agaagaacct taagaaaacc ttaagaagca ttgtttaggt ttcgtttaag ttaatcgacc
    60541 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    60601 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    60661 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgt cgtgagttaa
    60721 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    60781 gtaccgtcga gacggattgc ggaaccggtt atgtttatga tgtgattgct gattttgacg
    60841 gcaagccctc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacgttg
    60901 cacaactgcg ccaattactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    60961 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat tagtgagtca gaggttatcc
    61021 ccgtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcaaagt
    61081 ggtgttgtct gcgtaagcaa gagcggttgt ggaaacgatt tatcgcccaa ccggcgctgg
    61141 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcgcttcgc
    61201 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    61261 gccgtaaaat ccacgcctga cggctgcgca cgtagcccga tggcgcggag actttgaaac
    61321 gaagtggatt aggtaataca gctgccagta aggcagcttc cgatcgggta agtttgctcg
    61381 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    61441 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagacagtt tctatcccca
    61501 gcgttaatcc ggcttccagc ccttttcgaa cccagctacg cccatcccat aaaaagagat
    61561 ttttggctgt ctgttgagaa atcgttgaag caccgcgaat acggttttca ttgcgctcat
    61621 tgtgcgccag ggctttctca atggaagcga catcaaagcc ccagtgctca ggaaatgtct
    61681 gatcttctgc ggcaatcacc gccagtccca tccacggcga gatttgatcc atactgaccc
    61741 tgtcagaatg tgccacgtaa cgaaaattac catgcagcca ggcgctgacc tgtcgctcga
    61801 ccattaccgc agagaaggga acaggtgcaa cgctaaacaa cgcgatgccc ccgccccaga
    61861 aaacggcgag gacaaccatt aaccgcagaa gaaaacggcg aacgaaacta aacaccgtta
    61921 agcggctttt actcattcag ccagaaccag cacgcgggaa accagcttat caatgccgct
    61981 cgccgcttct gcaatgttct gcgccagcat atatgccggg gtggtgacaa tcttattgtc
    62041 ttcatcaacc acgatatcat cgacaggaca cggcacatgc tccgcgccca tctcttccag
    62101 cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat cgaaaatttt
    62161 cggcagcatc gccggggcaa tacacataaa accaagcggt tttccggcct gatgcatcgc
    62221 ttgtgccagc gcctttaatt cacggtcaac ggtgcattcg cgaccaagac tggcaaaatt
    62281 gcttaaattc tttgccgcgc caaacccccc cggcacaatc aacgcatcca gttcagcggc
    62341 atcggcctgg gccagaggac ggatttcacc gcgcgttatt cgtgccgctt caatcagcac
    62401 attgcgcgtt tccgtcatcg cttcgccagt taaatgattg ataacatcaa cctgctgctt
    62461 atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca acgtcaacac
    62521 cgcttcatga atttcagaac cgtcatagac gccgcatccg ctcagaatta cgccaatttt
    62581 cttcattatg atcatccttt cgcaattgac tgaaacacat taattttttt aataaaaatg
    62641 gtacgcatca cacatttaac tgattcatgt aacaaatcat ttaagttttg ctatcttaac
    62701 tgcgtgcggc ctgaaaaaca gtgcagtgcc cttgtaactc atcataataa tttacggcgc
    62761 agccaagatt tccctggtgt tggcgcagta ttcgcgcacc ccggtctagc cggggtcatt
    62821 ttttagtggc ttttgccacc cacgctttca gcacttctac gtcgtgacgc cactcttctt
    62881 tcatctcttc aatccattca ccgacgttat cttcccaggc gggaaggtca ggagactgaa
    62941 tttgctgacc cagctgttgc agatggcgca accccactga ccccgccgca cctttaattt
    63001 tatgtccttc ctcaacaatg ccttttttat cctgcgccgt cagattcgac tccagcacgt
    63061 tgacatagcc tggcatcatc ttctcaaaca ccgctaaccc gtcggtgatc agcttcggtc
    63121 ctacaagttc gagatactgt tccagcatgg gaatatcgag caatgcttct gatttactgt
    63181 tctcttctgt cgtcaccgta ctctcctcat catcctgggt atcccagaat ttcttgatca
    63241 tcgcggttag cgccggaaca gaaagcggct tactcagcac atcatccatt ccagcattga
    63301 ggtactcttg tttgtctttc agcacgttag cggttaaggc caccagcggc ggtaaatcct
    63361 ctcgcggata acgcttcgtc agttcacgag agatatccag cccggtcata tctggcaact
    63421 gaatatccag caacaccagg tcgtattcgc ccggtttaaa catctccagc gccgccttgc
    63481 cggtcatggc gacatcaacg ctgttaccta atttttccag cacagaacgc gcgacaatca
    63541 cgttcagttc aatgtcttcc accagcagca cattcagcgc cggtaaaggc atatcgtctt
    63601 catcaaacgc atcatcgacc tcttctgcta ccgacggtgc gtggatcgtc aacgtaaagg
    63661 ttgaaccttt accctgttcg ctggtaaccg taatatcgcc gcccatattt ttcgccagac
    63721 gacgagaaac ggccagacca ataccggtgc cggtggcagg tttaccgcca tgactgtctt
    63781 tcacctggta atacatggcg aagattttat ccagctcatc ctgcggaatg ccgataccag
    63841 aatcttccac ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc acggtaacct
    63901 ggccttgctg ggtgaatttg acggcgttac tgatgaggtt ccacaggatc tgccgtaaac
    63961 gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt cggctccagg ttaaagcgca
    64021 atcctttttg ttgcgcctgc aatgcggaga gattttccag atcggcaagg aagctggtga
    64081 aatcaaccgg ttgattatca agctggacct tgcgccgttc catcttatcc atgtcgataa
    64141 tatcgttaaa gatattcccc agcgtgacgg ccgaaacatg gatggtcttg agatattttt
    64201 cctgctcggc ggtgagttcg gtatccagca gaatgcggct caggccgacg ataccgttca
    64261 gcggtgtacg caattcgtgg ctgatggtgg aaataaacgt cgttttgtcg cggctggcgc
    64321 gttcaagcgc atcctgatac cgcttacgct cggtaatgtc gcgaccaaag cccatcaaac
    64381 cgtgacgttt acccacgcgg tcgtagtacg gcactttacg aatttcaaag caggctttgc
    64441 gtccgtcggg ataatccagc cactgctcat aggtcagtga cacattatga cggaacactt
    64501 tttcatcggt ttcaatgact ttcgcagcgg cttccggtga gtaaacatca gcaggtttca
    64561 ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg ttacagccgg
    64621 aaaactcttt atcttcgtta cgataaaaaa ccaggtcggg tgaagcatca aggaaggaac
    64681 gtaaaaatga ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa
    64741 ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga acggcaattt
    64801 cctgatttag ctgggcaata ttatctttta actgaacgtt gaggctcaaa tcgcgctcgc
    64861 gcatctcctc cagtttttgc accagccgtg acagacgttg tcgtgactcc tccagttgct
    64921 cgacgaccac cgatagaaag tagaccgccc acggcgtaat cagcaaacca aagaagatag
    64981 aacgaataac atcaatgctt tcgacctgac catgcagcac catggttacc gccatttgta
    65041 ccacaatggc aagaacgacg agggccagcg ccagcaacat tgagaagcgc accagaccta
    65101 acttcatcat caggtcaaca taatactgcg ccagcagacg aatttgcttc attagggaat
    65161 tccttcacga caacctgccc caataatacc caattctgac agttacgtta taaattgtgt
    65221 gagaaatgcg gaattcccct caacgaccta ctccgttgga ggtagccacg gacgtcccag
    65281 cgccgatccc tgtacgccat gttcattcag atagcgatcc agctcgacca ttcccgtcca
    65341 tcgattttcg caccacagag gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg
    65401 gtggtagatc acttccggtg gcgtatggcg aatcatttct ccggcagtga gcgtgtaatc
    65461 ctccagttca ataccgttca aacgccccgc ttcccaggct ttcgccataa tgctgccttt
    65521 cacaatatgc agcggatgca gctttatgcc gtccacgccg gtttcaacca ctcgctccag
    65581 cgtttgcaaa cattcagcct gcccttcgcc aggcaggccg acaattaagt gagaacacac
    65641 cttcagcccg cgctgacgtg ccagctgggt tgtacgctga taacaggcaa aatcatggcc
    65701 gcgattaatg cgatgcaggg ttttgtcgtg ggcggtttgt agccccagct ccagccacac
    65761 ttcgtagccc tggtccttat attcgcaaag cagatccagc accgcatccg gcacgcagtc
    65821 cgggcgagta ccaacacaca aaccgacaat attggcctgg ctcaccgcct gctgatacat
    65881 agaacgcagc acctgaactt ccgcaaaggt gctggtatac gcctgaaagt aggccagata
    65941 gcgtttagcg cggttaacta aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg
    66001 ttgctgcgct tcatcggcaa atgaggcaac attacagaat gtgcagccgc cacgcccgat
    66061 ggtaccgtca cggttagggc agctaaaacc gccgtgcagc gtcagcttat gcaccttttg
    66121 cccataacga cgggtgagat caccaccaaa catattgact aatttctgta actgcataat
    66181 ctgatagacc gcgccttgaa aagaggccaa agcctgccat ttttagctca attcggcgat
    66241 gacttggatc aatcgtcccg gcctgctttt atcaactgca taaccaatca aaattaccga
    66301 aatttcatgc ataatcacat aaatcacttt tgcttatctt gtgtcagatt tttttatctc
    66361 cttatggatt ttaggcaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    66421 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    66481 ggaattggct gtttctctaa cgacgcccct tttagcctta attagaaaat ccattttaat
    66541 ttcagtcaat taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    66601 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    66661 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    66721 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    66781 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    66841 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    66901 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    66961 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggtgacg gttgcggctt
    67021 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    67081 agcgaaaaac tacgccgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    67141 cgcacgccgc atcgttgaag aagagctgca acgcgaaacc ttgtcgattg tgggctggcg
    67201 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    67261 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtccg cgcgatatgg agcgccgtct
    67321 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    67381 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    67441 cttttatcta gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    67501 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct acctggcgca
    67561 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacttataa
    67621 attccagaca ccgcttatcc ctgacctgca cgacgccgca ccgttcgtca acgaaatcgg
    67681 ctctgactcc agttcgatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    67741 catccgcgcc atgcgtctat tagtaccacc cgcctggcag aacaacccgg atatggaccc
    67801 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggcccggc
    67861 gggcatcgtg atgtctgacg gtcgttttgc tgcctgtaac ctcgaccgta acggtctgcg
    67921 tccggcgcgc tacgtcatca ccaaagataa gctcatcacc tgcgcctctg aagtcggtat
    67981 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    68041 ggtaatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    68101 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    68161 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    68221 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    68281 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    68341 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    68401 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    68461 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    68521 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    68581 gctggatatc acctttgacg tcaccaaaac cacgctcgaa gcgacggtca aagagctgtg
    68641 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgctg ctggtgctct ccgaccggaa
    68701 tatcgctaaa gatcgcctgc cggttccggc tccaatggcg gttggcgcga tccagacccg
    68761 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    68821 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    68881 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    68941 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    69001 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctgca
    69061 caatgatgta gtgggcctgt gcttccaggg ggcggtcagc cgcattgggg gggcgagctt
    69121 tgaagacttc cagcaggatc tgctgaatct ctcgaaacga gcctggctgg cgcgcaagcc
    69181 catcagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    69241 ggacgtggtg cgcacgctgc aacaggcggt acagagcggc gagtatagcg actatcagga
    69301 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    69361 gccgggtgaa aacgcggtca acattgccga tgttgaaccg gcaagcgaac tgtttaaacg
    69421 cttcgatacc gcggcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    69481 ggaagcgatg aacagcatcg gcggaaactc gaactccggt gaaggtggtg aagacccggc
    69541 gcgctacggc accaacaaag tgtcgcgcat caagcaggtg gcttccggtc gctttggggt
    69601 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    69661 gccgggcgaa ggcggtcagt tgccgggcga caaagtcact ccttacattg ccaaactgcg
    69721 ctattcggtg ccgggcgtga cgctgatctc cccgccgccg caccacgata tctactctat
    69781 cgaagactta gcgcagctca ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    69841 cgtgaagctg gtttccgaac cgggcgttgg caccatcgcc accggtgtgg cgaaagctta
    69901 tgccgattta atcaccatcg caggctatga cggcggcacc ggcgcaagtc cgctttcatc
    69961 ggtgaaatac gcaggctgtc cgtgggagct gggacttgtt gaaacacaac aggcgctggt
    70021 tgccaacggc ttgcgtcata aaatccgttt gcaggtcgat ggcggcctga aaacgggtgt
    70081 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    70141 ggtcgcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg caacgggtgt
    70201 ggcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    70261 gaattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc ttggcgtaac
    70321 acgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    70381 caaacagcag aaactggcgc tgtcgaagct gctggagacc gccgaaccgc atccaggtaa
    70441 ggcactctac tgcaccgaaa ccaacccgcc gtttgataac ggcctgctga acgcgcagtt
    70501 gctgcaacag gcgaaaccgt ttgtcgatga gcgccagagc aaaaccttct ggttcgatat
    70561 ccgcaacacc gaccgctctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    70621 cgatcagggg ctggcagccg atccgattaa agcgtacttc aacggcaccg caggccagag
    70681 cttcggcgtg tggaacgcgg gcggcgtgga actgtacctg accggtgatg ccaacgacta
    70741 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    70801 ccgcagccat gaagcaagta ttatcggcaa cacctgcctg tatggagcga cgggtggtcg
    70861 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cgtaactccg gtgcaatcac
    70921 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta tcgtctgtat
    70981 tctgggtaaa accggcgtta acttcggtgc gggtatgacc ggcggcttcg cttacgttct
    71041 cgatgaaagc ggcgatttcc gcaaacgcgt taacccggaa ctggtggaag tattaagcgt
    71101 tgacgatttg gcgatccatg aagagcatct gcgtggtctt atcaccgagc atgtgcagca
    71161 taccggttcc cagcgtggtg aagagattct ggcgaactgg tcaaccttcg ccactaaatt
    71221 tgcgctggtt aaaccgaagt ccagtgatgt caaagcactg ctgggtcatc gtagtcgtag
    71281 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    71341 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgcaaaat
    71401 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    71461 ctgcctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    71521 gaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcacca
    71581 gaccaacact ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    71641 ttcctgcacc ctgaacgatg agtttggcgc ggtgaccatc ggcaacattg agcgttatat
    71701 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga aacagaccgg
    71761 taaaaaagtg gcgattatcg gcgcaggtcc ggcaggtctg gcgtgtgcag atgtcctgac
    71821 gcgcaacggc gtacaagcgg tcgtcttcga ccgtcacccg gaaattggcg gcttgctgac
    71881 cttcggtatt ccggctttca aactggaaaa agaggtaatg acgcgccgcc gtgaaatctt
    71941 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    72001 cgatctgctg agtgattacg atgccgtgtt ccttggcgtc gggacttatc agtcaatgcg
    72061 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    72121 caacaccaaa cagttaatgg gctttggcga aactcgtgaa gaaccgttcg tcagcatgga
    72181 aggcaaacgc gtggtagtac ttggcggtgg cgataccgca atggactgcg tgcgcacgtc
    72241 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    72301 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggt gtagagttca aattcaatgt
    72361 ccagccactg ggaattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtgcg
    72421 tactgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg tggcaggttc
    72481 cgaacatatc gttccagcag atgcggtgat catggcgttt ggtttccgtc cacacagcat
    72541 ggaatggctg gcaaaacaca gcgtcgagct ggattcgcaa ggccgcatca tcgccccgga
    72601 aggcagcgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    72661 ccgtggttcc gatctggtcg ttaccgctat tgccgaaggt cgtaaggcgg cagacggcat
    72721 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    72781 gctgaaattc tcctttcgtt ctggaaagcg cctcgcataa tctgaagaaa accaatgtaa
    72841 tacgcacatc tggattgtta ccggcagatg cccggcgtat agctgattcc attattcaac
    72901 actgcgccat ataaagggaa agataatgga atcgctctca gagagaacct caacaggcta
    72961 ccagcaaatc cacgacggta ttattcacct ggtcgatagc gcccggacgg aaacggtacg
    73021 tagcgttaac gcgctgatga ccgcgacata ctgggaaatt ggccgacgaa ttgtcgaatt
    73081 tgaacaaggt ggcgaggcca gggctgcgta tggtgcgcag ctaatcaagc gactatcaaa
    73141 ggatttaagt ctaaggtata agcgtgggtt ctctgcaaaa aacttacgcc aaatgaggct
    73201 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    73261 attgccctgg tccacttacg tccgtttact ttccgttaaa aacgctgaca cccgcagctt
    73321 ttatgaaaaa gagacgctcc gctgtggctg gtctgttcgc cagctagaac ggcaaattgc
    73381 gacccaattt tatgagcgaa cactgctgtc acatgacaaa tcagccatgc tgcaacaaca
    73441 cgctcctgcc gagacgcata ttcttccgca acaggcgata cgcgatccct ttgtgctcga
    73501 atttctggaa ttgaaagatg aatactcaga atccgatttt gaggaggcgc tgatcaacca
    73561 cctgatggat ttcatgctgg aacttgggga tgattttgcc tttgttggtc ggcagcgaag
    73621 gttacgcatt gatgacaact ggtttcgtgt cgatctgctg tttttccacc gccgtttacg
    73681 ctgcctgcta atcgtcgatc taaaagtggg caaattcagc tatagcgatg ccggacagat
    73741 gaatatgtat ctcaactacg ccaaagagca ctggacgcta ccggatgaaa atccgcccat
    73801 cggtctggtt ctctgtgcag agaaaggagt cggagaagcg cattatgcac tggctggttt
    73861 gcctaacacc gttctggcga gtgaatataa gatgcaacta cctgatgaaa aacgactcgc
    73921 ggatgaactc gttcgaacac aggcggtgct agaagaaggc tatagactcc gttaatttca
    73981 gcggtttcga attctgcaga cactgtctgc agaatcttta aaacgaacta agttttaagc
    74041 cattaaatca gccttaacct tcaccaccac ctttttaatc tcaccaggtt cgccgacaac
    74101 gcatcctggt ttatgcggtt ctcccggcat aaacacggcg aacattcccg gttttaagtt
    74161 gatggtttgc tcgttttcaa tggcgctgca aagctggtaa tcatcttcat ggtggaactc
    74221 ttcacactgg cgcgcagtgc ctgccgtgcc aaacagaatc cgttcctcac cttttaataa
    74281 cagctggata tcaatgtatt gctcgtgcaa ttccgctttt ttctcgacgg gcgattgagt
    74341 gttaaacgtc atgacattca taaaaatatt gtcgccctgt aattcgtaac gacccggcgc
    74401 tttttcttgc ggtctggcag ctaatgccag cgttaacgcg tcctgtaacg caggatgtaa
    74461 cccagcagac ggtaatgact gtacttcacc catcatcata atttttctcc ctgggccaac
    74521 agcgcagccc caagtaaacc tgcatcatgg cggtagtgcg ccgccagtaa atcaacatga
    74581 aatgccgctg gctcctgcgc cagatacgtt tccaccagcg ccagataccc ttctgccaga
    74641 ccaacgctgc caccgaccac cacgcactga caatcagttg tggctttaat atcagcgatc
    74701 agccttgcca gcacatgtgc ggagcggtga atcagctgct gcgcctgctc gtcgccctgc
    74761 ccggcgcgca tgaaaatagt tttcgcatcc gcgccagcca actccccctg cgctgccgct
    74821 gcaataccgc gaccagaggc aatcgcttcc acgcaacctg tgcgtccaca gccgcagact
    74881 gggccgtgtg gatcggcaag cgtatgcccg atgtgccccg ccagaccgcc agggccggta
    74941 agcagtttgc cgccgctcac tacaccgccg ccgacgccag tggaaacggt gataaacacc
    75001 atatcggtta tatcgccttc cagcgcctga tactctgccc atgctgcggc ctgggcgtcg
    75061 ttaatggcaa tggtcggcaa atcggtaagt tgccccagcg ttttgactaa cggaaagtgt
    75121 agcaatccac caagattatg cggattcagc gccagcaaac tgccgtcgcg aataattccg
    75181 gttgaagcga tggcaacccg ctgcgcatga gcttgcaacg gagagactaa tgcggataag
    75241 gcatcacgca aggcttctgg tgtctggctg gctggcgtag gaagttcacg acgatcgcgg
    75301 atctgcccgt cagcgccaat cagcgcggcg gcaagtttag taccgccgat atcaatcgcc
    75361 agtgtggtca tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa
    75421 gacgcgtaat ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc gcatcagccg
    75481 cctgagcagg cgtgttgtaa cgcccttcgg caatcacccg acatccggcg tcgctcaacc
    75541 ttttcaccag cgtcagatcc ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg
    75601 tagtgccaat aatttcggct cccagctttt ggcatgccag gccgtcttcc ggcgttgagc
    75661 agtcggtcat cgccagtaaa ccgtgatgat gaatacgtgc cagcagcgtt tcaacaggca
    75721 ccggacgcgg gcggtcggtg ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg
    75781 catcgacatc ttcaatatag gccgtgatgc gtaccggaga atcctccaga tcgcgtttca
    75841 caattccaat aatcggcacg ctcaccaccg cacgcgtggc ttgcagattt gccacacctt
    75901 cgatgcgaat ggcaaccgcg cccgcctgct ctgccgctaa tgccatggct gcgacgattt
    75961 cgggtttatc gagcgggctg tccggaaccg gctggcagga gacaatcagg ccaccgttag
    76021 cagcgatttt ttgatccagt tgtgcaagta acgacataca tcttccctta gcgaaaggcc
    76081 cgatacagag accgggcaac aggattaact tttggttttg actaaatcgt ttttggcgct
    76141 gccaaacggc acggcaccgc taaatggttt gccgtcgata gcgtcatgag tacgcaacgc
    76201 ttccgggcgc agccaacgct gaacacgaga aggcatatcc agaccaatca gcaggatcac
    76261 cacaaatgtc agactgaacg agagcgatgc cagtgcagta cccagatcca gacgttgagc
    76321 gatcaacgcg ccgatgattg gggccagtgc gccacccaat gcgccaacgt tataggtaaa
    76381 gcccaggccc gctgcacgct ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat
    76441 cccggcgatc ccttgtccaa gcatttgctg gaagaacagt aacagaccga gcacccagac
    76501 gtttgcaccg ccaatagcaa ataccggaat aatcagcagc tgcgaggcca acagactaca
    76561 aacgtacgct ttgcgggttc ccagccagtc accgaggaag ccaccgacgc agcaccccac
    76621 cgccgcgcca aagccactaa agaacagcac attggcgaca gtatgagggt tataagccag
    76681 atcggttttc agatacgttg gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa
    76741 caacacgacc accatcagca ttacgccagt tggccagcgt ttgcccgtac tctgcaccat
    76801 aaagctgata aagattgcgg tgcataacag cccaagaaca gcgacgatcg cggcattttg
    76861 caggttaccg gcgaagcaga accacagcgc agtagccgcc gccagtgtca ttacgatatt
    76921 ggcaatgcga tgttcaccac ggtagagaat atccaccatt gtgcgtactg gtgctttacc
    76981 tgcgtgtttc tctttccagt cttctgcttc cgggatgttt ttacgcagcc agagagcaaa
    77041 gatgattggc aaaatgccga taaagaacag cgcacgccag ccccagaccg gaaccaccag
    77101 gctgtagacc tgagcggcaa cgacggcccc tacagagaag cctgaaatca gaaagccact
    77161 ggctttgtta cgcagatgtt ttggccagct ttcaatgaca taggtggcgc tggaaccgta
    77221 ttcacccgcc atccccatgc cgatgaccag acgagcgata aacatggtga tgtagcctgg
    77281 cgcaaagccg catgccagcg tcccggccga gaagagaacg atgctggtga ccattgccag
    77341 acgacgcccg tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc agcgagagat
    77401 aaaggctgcg gagatcagac ttgccgcctg caccgtcgtc agcccgaatt caccttgtac
    77461 ttcggtgagt accagggcga ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca
    77521 ggcagcggaa aatgcccgcc attgtgcacg attgagatgg cgataccacg ggatattctg
    77581 ggttgtagta ctcattgtga gtctcccgcg gtgggcgatg cccacacgct ttggtatgaa
    77641 aattgtaggg tacagatgcg tttatttccc ctcacccggt aggggcgagc gaggggaaac
    77701 aactcacccg cgctcttgca tcaactgctg ggccagcgcc ttcagttctg gcagatattt
    77761 ttcatctacc ggaccaaacg gtttgcggca cagcggcaca gaaacgacat ccatataatg
    77821 cagaacagtt ttcaggccgc ggaatacacc cgttttgatc agtaaatcaa tgactttgtt
    77881 gcattcagtt tgcagtttct gcgcggtctg gatatcgcct tctttcagcg ccttaacgat
    77941 cccctgatag cgccagccca tgatgttgta ggtactaccg ataccaccat cagcgcccgc
    78001 cagcaggcca gaggcgaaga tttcgtcgta accgttatag agcaccagat caggatgttc
    78061 acgacggatc tgctccatct gatagagatc gccagaggtc tgtttcagcg cacctacgcc
    78121 aggcaatgta acaagtgtgt tgatctgatc cagggtcagt tttaccccac tcagggctgg
    78181 aatgttgtac accaccatcg gcaaaccatc cgccgaatca ataattgccc gatagtgatc
    78241 gcagtgttct tcaaagctga aaggatagta gaacggcgtg acggcggaga cggcatcgaa
    78301 gccataacgt ttagccgatg ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac
    78361 gtgggcgatg agtttaatct tacctttcgc ctcttcggcg acgatttcca gtacctgttc
    78421 acgctcggaa aggctttgta caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc
    78481 aatgccctgc tgaatattga actgaaccag gcggcgcaga ctcgctttat ccagtgcttg
    78541 ttgttgatca aaaggagtca ggagtgcagc cattacgcca cgtaaattcg ttgccataaa
    78601 tacctctgaa gtgatgcttg tctgataaac gatatacctt tatacctgtt ataccagatc
    78661 aattaagcaa caccccatac agaaagctta taatgcgatc tgcttcacta aagtggcatt
    78721 atttcttttt gttggtggtc tgaccgaagg cgtgccaggt agcagagacg ctgttgagat
    78781 gcgattgcaa cgcacgatcg gcttcgtcag gatcgtggcg gcggatcgcg tcaacgatcg
    78841 caatatgctg ttgatagcta acgttgttat gctcgtgcag agcctgatca gccacggttg
    78901 gacgtgcggc gatcagccag tcgagcaggg caacgtggat cgccatgaag attgggttac
    78961 cggggatctc cgccagcacg cggtggaaat caacgtctga gcgaatgaat gccgcgttgt
    79021 tatccagcga ctgactgttg atttccagtg cttttgccag caaatcgatt tgctcatcgg
    79081 tggcgtgttc agccgcatag cgcaccaggc tggattcaaa gaacagacgt aattgttcga
    79141 aatgggcaat cccaccagga tgagaaagga aatctttcgc catgccggaa agctcgccga
    79201 tgatggtgtc cgcagaagga cgtgagacgc gggcacgttc gccgttgttt atttgcacca
    79261 gacctttgcg ttttaacgct gccagcgctt cacgcaccga aggacgcccg acgttaaaga
    79321 acgccatcag ttcgcgttca gacggtaatt gttcaccttc gccaaattca cgacggcgga
    79381 tcatctgttc cagctcttct tccaccattt cggagagttt tttacgcgcc agcgggcggc
    79441 tacgcaagtt gcgaccaatt gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca
    79501 taaggcccat tctgtaaggt cagtgtgatt aacatcatca gtgacatcct atcacaggat
    79561 tgaaagtagg ggaaaatggc agggttttct ctttgtgcct catcattacc ataattaacg
    79621 gaataattaa ctattgcgaa aaaataatgt aacgcagata aaaacatccc gtttgaatta
    79681 tttataagac tattcacgag cattatgaat attatgaatg cgttcttaca aaataatcat
    79741 aagcgcatat tttttaatga aaaatcacct cacctacaat taaaaacacg acatccgcac
    79801 cataaataga cttgcaaaaa atataacatc attgttttca atctgccgtt tatgggattg
    79861 acccttttct tttgacacgg agttcaacaa tgttcggcat aattatatct gtcatcgtat
    79921 taattacgat gggctatttg atcctgaaaa actacaaacc tcaggtggtg ctggctgcag
    79981 gtatcttcct gatgatgtgc ggtgtctggt tagggttagg tggtgtactc gatcccgcca
    80041 aaagcagcgg ctacttgatc gtcgatattt ataatgaaat cctgcgcatg ctgtccaacc
    80101 gcattgccgg attggggctg tcgattatgg cggtgggcgg ttatgcccgc tacatggagc
    80161 gcacaggagc cagtcgcgcg atggtgagct tgttaagccg cccgttaaaa ctcattcgct
    80221 cgccgtatat tattctgtcg gcaacttacg tcatcggcca aatcatggcg cagtttatta
    80281 ccagcgcctc cggcctgggt atgttgctga tggtcacctt atttccgaca ctggtgagtc
    80341 tgggagtaag ccgtctctct gcggtggcgg ttatcgcaac cacaatgtcc attgagtggg
    80401 ggattctgga aacgaactcc atttttgcag cccaggtagc gggaatgaaa attgccactt
    80461 acttcttcca ctaccagctt ccggtcgcct cttgcgtcat tatctcggtg gcgatctccc
    80521 actttttcgt gcaacgcgct tttgacaaaa aagataaaaa tatcaatcac gaacaggcag
    80581 agcaaaaagc tctcgataat gtcccgccgc tctattacgc cattttacct gtgatgccgt
    80641 taatcctgat gctcggctcg ctattccttg cccacatcgg gctgatgcag tcagaactgc
    80701 atctggtggt ggtgatgtta ctgagtttga cgatgtttgt tgagttcttc cgcaagcata
    80761 acttgcgcga aacaatggac gatgtgcagg cgttttttga cggcatgggt acgcagtttg
    80821 ccaacgtggt aacgctggtg gtcgcgggtg aaatatttgc gaaaggctta acgacgattg
    80881 gcactgtcga tgcggttatc aggggtgcgg agcattctgg tctgggcggt attggcgtga
    80941 tgattattat ggcgctggtc attgccattt gtgccattgt gatgggctct ggcaatgcgc
    81001 cgtttatgtc atttgccagt cttattccga atatcgcagc cggactacat gtaccagcgg
    81061 ttgtaatgat tatgccgatg cattttgcca cgacgctagc gcgcggtttc gccgattact
    81121 gcggtggtgg tcgttacgtc aggaattgca ggcgtttcgc cttttgcggt ggtgaagcgg
    81181 acagcgatcc ccatggcagt cggtttcgtg gtgaatatga ttgccacaat cacgctattt
    81241 tattaagtca ttaaaagaca aaacaggccg ccagggcctg tttttgtatt acttcacaac
    81301 gcgtaatgcc ggtcgaccac cgcgtggtgg ctgcggtggt tcatcgtcag gatgagtgtc
    81361 atcatcgtga tctggcttgt cgccatcaat aaccgacata acggtttcgt tgtctgccga
    81421 tgcctcttca tcattcatga tgctggtatc ttcatcgtag gcagcttcag gctcaaacat
    81481 cgtgcctgcg ccattttcac gggcgtagat agccagcacg gcagccagcg gcacagaaac
    81541 ctgacgcgga atgccaccaa agcgcgcgtt aaagcgcacc tcatcattcg ccagttccag
    81601 attgccgaca gcacgcggcg caatgttgag tacgatttgc ccgtcacgcg catattccat
    81661 aggaacctgc acgccaggga gcgtcacatc caccaccagg tgcggcgtga gctggttatc
    81721 cagcaaccac tcatagaatg cacgcagcag atagggacga cgtggtgtta gctgtgacaa
    81781 atccatacag attaactccg gcccagacgc atttcacgtt ctgcttcagt taaagaagca
    81841 aggaaagagt cacgctcaaa gacgcgggtc atatagcctt tcagctcttt cgcacccggg
    81901 ccgctgaact cgatacccag ttgcggcaga cgccacagca gcggagcaag gtagcaatcg
    81961 accaggctga actcatcgct caggaagtac ggcttctgac cgaagaccgg cgcaatcgcc
    82021 agcagttctt cgcgcagttg cttacgtgcg gcatctgctt cagaagctga accgttgatg
    82081 atggtgttca tcagcgtgta ccagtctttt tcgatgcgat gcatgtacaa acggctttca
    82141 ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc atccagatat
    82201 tccataatga tgcgagattc ccacagggtc agctcacgat ccaccagggt cggaacgctc
    82261 tgattcgggt tgaggtcaat cagatcctga ggcggattgt ccttttccac gtgttcgatc
    82321 tcgaaactta cacctttctc agccagcaca atgcggacct gatggctata gatgtcagta
    82381 ggaccggaaa acagcgtcat taccgaacgt ttgttggcag cgacagccat gaaaacctcc
    82441 aggtatagtc agaattttta ctgctaccag ccaccaggtg gccagtcaga agttgtgtta
    82501 cccagtaagg aacgactctc tttgttcgaa aatcaaacaa aaaatgagca atacccgaca
    82561 tttgggcaga aaattggatg atagtttacc agattttgcg acctttgtgg tgagtcgatg
    82621 ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata gttgacttag tcccttattg
    82681 gcgatgtggt ttttgttttg cctgtctgtc agacggcagc aaaaagcaac tttccatttt
    82741 ttacgctgat tcagaattta accataaaaa aacccgccga agcgggtttt ttcgcaaatt
    82801 gttttctgcc ggagcagaag ccaattaacg tttggagaac tgcggacgac gacgtgcttt
    82861 acgcagaccg actttcttac gttcaacctg acgagcgtca cgagtaacga agccagcttt
    82921 acgcagttca gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga taccgtgacg
    82981 gatcgcacca gcctgaccag agataccacc acctttaacg gtgatgtaca ggtccagttt
    83041 ctcaaccata tcgaccagtt ccagcggctg acgaactacc atgcgagcag tttcacgacc
    83101 gaagtactgt tccagagaac gttggttgat tacgatttta ccgttgcccg gtttgatgaa
    83161 aacgcgagct gcggaacttt tgcggcgacc agtgccgtag tattgatttt cagccattgc
    83221 ctataatccc gattagatgt caagaacttg cggttgctgt gccgcgtggt tgtgctcgtt
    83281 acccgcgtaa actttcagtt tacggaacat agcacgaccc agcgggcctt ttggcaacat
    83341 gcctttaacc gcgatttcaa tcacacgctc aggacggcga gcaatcatct cttcaaaggt
    83401 cgcttgtttg ataccaccga tgtggccggt gtggtgatag tacactttgt cagtacgctt
    83461 gttgccggtt acagcaactt tgtcagcgtt cagaacgatg atgtaatcac cggtatctac
    83521 gtgcggagtg tattccgctt tgtgcttacc gcgcaggcga cgagccagtt cagtagccag
    83581 acggcccaga gttttaccgg tcgcgtcaac aacataccag tcgcgtttta cggtttctgg
    83641 tttagctgta aaagttttca ttaaaagctt acccaataaa tagttacacg ttggtgaaca
    83701 cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct accccttcga
    83761 atagcctatg ccagcacaca aaaagttttg ggaaaaaaac tttcttgtaa cgtggggtcg
    83821 caggattata gagaagtcgg ggtcaaagat cgaccccttt ttgtgatttg tggcaggttt
    83881 taacccgcta aatgctcgcg cttcagatac tcttcgcttt gcatctcttg cagacgtgac
    83941 aggcaacgct ggaactcaaa cttcagccga tcgccctgat aaatttcata cagcggcact
    84001 tctgcactca ccactaattt gacatggcgc tcgtaaaact catccaccag cgcaataaag
    84061 cgccgcgctt cgctctccat caaccgcgtc ataactggta catcaaacaa catgaccgta
    84121 tgaaagagac gtgagagcgc aatatagtca tgctgactgc gggcgtcgac gcacagcgta
    84181 gtaaaagaga ccgccagcgt ctggttctcg acgcccattg tcgctaatgg ccgatggttg
    84241 atttctaacg tcggtgaatt ttctcgtttc ccccccgcca gcgccaacca tagtttatcc
    84301 atttgcgccc gggtttcatc gtggagtggc gaaagccaca gatgcgcctg agtgagtgta
    84361 cgcagacgat aatcaacacc agcgtccacg ttcattacat cacaatgctg tttaatggca
    84421 tcgattgcag gcagaaaacg cgcacgttgc aagccatttc gataaagttc gtccggcgga
    84481 atatttgacg tcgctaccag ggtaataccg cgggcgaaca gggctttcat caggccgcca
    84541 agtagcatgg catcggtaat atcagagaca aaaaattcgt caaaacagag cacgtcagtt
    84601 tctgctttaa aacgatcagc aataatttcc agcggatcgg tctgcccctg taaggcagtt
    84661 agctcttcat gcacccgcag cataaaacgg tgaaagtgca ggcgctgttt ccgctctccc
    84721 ggcaggcttt gatagaaaag gtccatcagc caggttttcc cgcgtcctac accgccccac
    84781 atatataagc cacgcactgg cgtatgcttt gtgtcttccc gtttacccca cagcttaccg
    84841 acccgcgcca ttagcccact cgtcctgggg gctggtggcg tgctattgat gagttcctga
    84901 taaataattt ccaagcggct gacggcctct ttttgaacgt cgtcgggttg atggctgcct
    84961 tcattgagcg ccttcaggta ttgcgatgtt ggagtaacgc tttgcatgat cttattgtta
    85021 ttccttgaat aatcggtgcg ccgttgttca cggttgacga aaaaaaggcc gttctacact
    85081 acgcgatatg cagtcgggat tccacttctg tggaattaac ggttatagtg gcataatcag
    85141 ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca aaagacaacg ggagatgttc
    85201 atgacctggg aatatgcgct aattgggtta gtcgtcggca tcattattgg tgctgtggcc
    85261 atgcgttttg gtaatcgtaa actacgccag caacaggcgt tgcagtacga actggaaaag
    85321 aataaagctg aactggacga gtatcgcgaa gagctggtta gccactttgc ccgcagcgcg
    85381 gaattactgg ataccatggc gcacgactat cgccagctgt atcagcacat ggcgaaaagc
    85441 tccagcagcc tgctgccgga actgtctgct gaagcaaacc cgttccgtaa tcgtctggca
    85501 gagtctgaag ccagcaacga tcaggcaccg gtgcagatgc ctcgcgacta ttctgaaggc
    85561 gcatccggcc tgctgcgtac tggcgcgaag cgcgactaat ttatttttcg ggcgcagcca
    85621 ttgcgccctc ctcttctctc cctccccgac tatcatttaa tctggtgtct cattgttagc
    85681 cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct tttcttatca ttcaggtacg
    85741 agagcaggaa taatgaaaaa acaaacccag ctgttgagtg cattagcgtt aagtgtcggg
    85801 ttaactctct cggcgtcatt tcaggccgtt gcgtcgatcc caggccaggt tgccgatcag
    85861 gcccccctcc ccagtctggc tccaatgctg gaaaaagtgc ttccggcagt ggtgagcgta
    85921 cgggtggaag gaacggccag tcagggacag aaaatcccgg aagaattcaa aaagtttttt
    85981 ggtgatgatt taccggatca acctgcacaa cccttcgaag gtttaggctc cggtgtcatc
    86041 atcaacgcca gtaaaggcta tgtgctgacc aacaaccatg tgattaatca ggcacagaaa
    86101 atcagtattc agctcaatga tgggcgcgag tttgatgcaa aactgattgg tagcgatgac
    86161 cagagcgata tcgccctgtt gcaaattcaa aacccgagca aattaacgca aatcgctatt
    86221 gccgactccg ataaattgcg cgtcggtgat tttgccgtgg cggtcggtaa cccatttggc
    86281 cttgggcaaa ccgccacctc tggcattgtt tccgcattag gacgcagcgg gttgaatctt
    86341 gaaggtctgg aaaactttat ccagacagat gcttccatta accgcggtaa ctccggcggt
    86401 gcactgttaa accttaacgg tgagttaatt ggcatcaaca ctgcaatcct tgcgcctggc
    86461 ggcgggagcg tcggaattgg atttgccatc cccagtaata tggcgcgaac actggcgcag
    86521 cagcttatcg actttggtga aatcaaacgc ggtttgttag gcatcaaagg caccgagatg
    86581 agtgccgata tcgccaaagc cttcaacctt gacgtgcagc gtggcgcgtt tgtcagcgaa
    86641 gtgttgccag gttctggctc ggcgaaagcg ggcgtcaaaa cgggcgatat tattaccagc
    86701 ctcaacggca aaccgctgaa tagctttgct gagttgcgct ctcgtatcgc gaccaccgag
    86761 ccgggcacga aagtgaagct tggcctgctg cgtaacggca aaccactgga agtagaagtg
    86821 acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa tgatcacgcc agcgctggaa
    86881 ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca aaggtattaa aatcgatgaa
    86941 gttgtcaaag gaagcccagc tgctcaggct ggcttgcaaa aagacgatgt gatcattggc
    87001 gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta aagtgctggc ggcaaaaccg
    87061 gccatcatcg ccctgcaaat tgtacgcggc aatgaaagca tctatctgct gatgcgttaa
    87121 tgtcgtaaac cgggcatcag gctgacgtgt gatgtccggt taactcgtgg tatgctgctg
    87181 ccgttccctt ttttaatgac gcctccatca tgtttgtgaa gctcttacgt tccgttgcga
    87241 ttggattaat tgtcggcgct attctgctgg ttgccatgcc ttcgctgcgc agccttaacc
    87301 cgctttccac tccgcaattt gacagtaccg atgagacgcc tgccagttat aatctggcag
    87361 ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg tggtttgaac accaactctc
    87421 acaaccagct tgagatccgc accctgggat ccggtgtaat catggatcaa cgcggttata
    87481 tcatcaccaa taaacacgtc atcaacgacg ccgatcagat catcgtcgcc ttacaggatg
    87541 gacgtgtatt tgaagcattg ctggtgggat ctgactctct aaccgatctg gcggtactta
    87601 aaattaatgc cactggcggt ttacctacca ttccaattaa tgcacgtcgc gtaccgcaca
    87661 ttggcgacgt ggtactggcg atcggtaacc cgtacaacct cgggcagacc attacccagg
    87721 ggattattag tgctacgggt cgaatcggtc tgaacccgac cgggcggcaa aacttcctac
    87781 aaaccgatgc ttccattaac cacggtaact ctggcggcgc gctggtgaac tcgctgggcg
    87841 aactgatggg cattaacacg ctgtcgtttg ataagagtaa cgatggcgaa acgccggaag
    87901 gtatcggctt tgcgattcct ttccagttag caaccaaaat tatggataag ctgatccgcg
    87961 atggtcgcgt gatccgcggc tacattggta tcggcggacg cgagatcgca ccactgcacg
    88021 cgcagggcgg tggtatagat caactgcaag ggatcgtggt taatgaagtg tcacctgacg
    88081 gcccggcggc gaatgcgggt attcaggtca acgatctgat tatttcggtg gataacaaac
    88141 cggccatctc tgctctggag acgatggatc aggtggcaga aattcgccct ggttcggtga
    88201 tcccggttgt agtgatgcgt gatgataagc agttaacgct gcaggtcacc attcaggaat
    88261 atccggcaac caattaaggc cagcgtgaaa gacaaaaaaa ccggagtctg tgctccggtt
    88321 ttttattatc cgctaatcaa ttacttatta acgaactctt cgcccagggc gatatctttc
    88381 ttgagcgtat ccagcatacc ttccagcgcg ttctgttcaa atgcgctcag ggtaccgata
    88441 gatttacgct cttccacgcc gtttttaccc agcagaagcg gctgagagaa gaaacgggcg
    88501 tactgaccgt cgccttcaac gtaggcacat tcgacaacgc cttgttcgcc ctgcagtgcg
    88561 cgaaccagag acaggccaaa acgtgcagca gcctggccca tagacagggt tgccgacccg
    88621 ccaccggctt tcgcttcaac cacctcagta cccgcgttct ggatacgttt ggtcagatca
    88681 gccacttcct gctcggtaaa actaacgcca ggaacctgtg acaacagcgg cagaatggta
    88741 acaccagagt gaccgccaat aaccggcact tcaacttcgc ctggctgttt gcctttcagt
    88801 tccgcaacaa aggtgttgga acgaatgata tccagcgtgg taacgccgaa cagtttgttt
    88861 ttgtcataaa caccggcttt tttcagcact tcagcagcaa tcgcaactgt ggtgttaacc
    88921 gggttagtga taataccaat gcacgctttc gggcaggttt tcgcaacttg ctgtaccagg
    88981 tttttcacga tgccggcgtt aacattaaac aggtcggaac gatccatacc cggtttacgc
    89041 gctacacctg cagagataag aacgacatct gcgccttcca gcgccggagt cgcatcttca
    89101 ccagaaaaac ctttgatttt cacagcagta gggatatggc tcagatcgac ggccacaccg
    89161 ggagtcactg gagcgatatc atacagagag agttctgaac ctgaaggcag ttgggttttt
    89221 aacagtagtg caagcgcctg gccaataccg ccagcagcgc cgaggactgc gactttcatc
    89281 ctaaactcct tattatattg ataaactaag atatgttgct ccgctgccgc gaccttaatc
    89341 cacaaaattg ccgtttacaa tgaccacatc tcaagaatgt gtagtcacgc aagtttagcg
    89401 tttatgcatt taattgccgt aatcaggaac ctaagttacg taattaacaa tcgcttatgc
    89461 attagcgcaa cattccaaca ggtggtgaca atatacccta ccgttcagcc aaaacaacat
    89521 caatttgata acaattaatt tacttttaag cagaatttgc atgccgtgac gcaggcatat
    89581 ttctcaataa cgaaatttga taaaatcccg ctctttcata acattatttc agccttcttc
    89641 agggctgact gtttgcataa aaattcatct gtatgcacaa taatgttgta tcaaccacca
    89701 tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa ctagttaaag catttaaagc
    89761 attacttaaa gaagagaaat ttagctccca gggcgaaatc gtcgccgcgt tgcaggagca
    89821 aggctttgac aatattaatc agtctaaagt ctcgcggatg ttgaccaagt ttggtgctgt
    89881 acgtacacgc aatgccaaaa tggaaatggt ttactgcctg ccagctgaac tgggtgtacc
    89941 aaccacctcc agtccattga aaaatctggt gctggatatc gactacaacg atgcagttgt
    90001 cgtgattcat accagccctg gcgcggcgca gttaattgct cgcctgctgg actcactggg
    90061 caaagcagaa ggtattctgg gcaccatcgc tggcgatgac accatcttta ccacccctgc
    90121 taacggtttc accgtcaaag acctgtatga agcgatttta gagctgttcg accaggagct
    90181 ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt tgcttatccc tctcaacccc
    90241 ctgctttccc ctgcgattaa tttaacgaat agtgcgtttt actgcgacat gt
//
LOCUS       DABSYT020000009        90126 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.009, whole genome shotgun sequence.
ACCESSION   DABSYT020000009 DABSYT020000000
VERSION     DABSYT020000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 90126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.009"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            46..777
                     /locus_tag="ID725_RS04365"
                     /old_locus_tag="pgaptmp_000873"
     CDS             46..777
                     /locus_tag="ID725_RS04365"
                     /old_locus_tag="pgaptmp_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGV7849534.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            913..1833
                     /gene="speB"
                     /locus_tag="ID725_RS04370"
                     /old_locus_tag="pgaptmp_000874"
     CDS             913..1833
                     /gene="speB"
                     /locus_tag="ID725_RS04370"
                     /old_locus_tag="pgaptmp_000874"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGV7849535.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITTLAAATLALEMLYIQAAKKGE"
     gene            complement(2039..2797)
                     /gene="loiP"
                     /locus_tag="ID725_RS04375"
                     /old_locus_tag="pgaptmp_000875"
                     /gene_synonym="yggG"
     CDS             complement(2039..2797)
                     /gene="loiP"
                     /locus_tag="ID725_RS04375"
                     /old_locus_tag="pgaptmp_000875"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGV7849536.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            3075..5066
                     /gene="tkt"
                     /locus_tag="ID725_RS04380"
                     /old_locus_tag="pgaptmp_000876"
     CDS             3075..5066
                     /gene="tkt"
                     /locus_tag="ID725_RS04380"
                     /old_locus_tag="pgaptmp_000876"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGV7849537.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            5380..5823
                     /gene="cmtB"
                     /locus_tag="ID725_RS04385"
                     /old_locus_tag="pgaptmp_000877"
     CDS             5380..5823
                     /gene="cmtB"
                     /locus_tag="ID725_RS04385"
                     /old_locus_tag="pgaptmp_000877"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGV7849538.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            5851..7239
                     /gene="cmtA"
                     /locus_tag="ID725_RS04390"
                     /old_locus_tag="pgaptmp_000878"
     CDS             5851..7239
                     /gene="cmtA"
                     /locus_tag="ID725_RS04390"
                     /old_locus_tag="pgaptmp_000878"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGV7849539.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            7254..8531
                     /gene="yggP"
                     /locus_tag="ID725_RS04395"
                     /old_locus_tag="pgaptmp_000879"
     CDS             7254..8531
                     /gene="yggP"
                     /locus_tag="ID725_RS04395"
                     /old_locus_tag="pgaptmp_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGV7849540.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            8528..9493
                     /gene="glpX"
                     /locus_tag="ID725_RS04400"
                     /old_locus_tag="pgaptmp_000880"
     CDS             8528..9493
                     /gene="glpX"
                     /locus_tag="ID725_RS04400"
                     /old_locus_tag="pgaptmp_000880"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGV7849541.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            9515..10024
                     /gene="fumE"
                     /locus_tag="ID725_RS04405"
                     /old_locus_tag="pgaptmp_000881"
     CDS             9515..10024
                     /gene="fumE"
                     /locus_tag="ID725_RS04405"
                     /old_locus_tag="pgaptmp_000881"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGV7849542.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            10021..10734
                     /locus_tag="ID725_RS04410"
                     /old_locus_tag="pgaptmp_000882"
     CDS             10021..10734
                     /locus_tag="ID725_RS04410"
                     /old_locus_tag="pgaptmp_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGV7849543.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10706..11383)
                     /locus_tag="ID725_RS04415"
                     /old_locus_tag="pgaptmp_000883"
     CDS             complement(10706..11383)
                     /locus_tag="ID725_RS04415"
                     /old_locus_tag="pgaptmp_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGV7849544.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(11377..12054)
                     /locus_tag="ID725_RS04420"
                     /old_locus_tag="pgaptmp_000884"
     CDS             complement(11377..12054)
                     /locus_tag="ID725_RS04420"
                     /old_locus_tag="pgaptmp_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGV7849545.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(12042..12749)
                     /locus_tag="ID725_RS04425"
                     /old_locus_tag="pgaptmp_000885"
     CDS             complement(12042..12749)
                     /locus_tag="ID725_RS04425"
                     /old_locus_tag="pgaptmp_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGV7849546.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(12750..13328)
                     /locus_tag="ID725_RS04430"
                     /old_locus_tag="pgaptmp_000886"
     CDS             complement(12750..13328)
                     /locus_tag="ID725_RS04430"
                     /old_locus_tag="pgaptmp_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGV7849547.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(13351..13782)
                     /locus_tag="ID725_RS04435"
                     /old_locus_tag="pgaptmp_000887"
     CDS             complement(13351..13782)
                     /locus_tag="ID725_RS04435"
                     /old_locus_tag="pgaptmp_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HGV7849548.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            14154..15173
                     /gene="epd"
                     /locus_tag="ID725_RS04440"
                     /old_locus_tag="pgaptmp_000888"
     CDS             14154..15173
                     /gene="epd"
                     /locus_tag="ID725_RS04440"
                     /old_locus_tag="pgaptmp_000888"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGV7849549.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            15223..16386
                     /gene="pgk"
                     /locus_tag="ID725_RS04445"
                     /old_locus_tag="pgaptmp_000889"
     CDS             15223..16386
                     /gene="pgk"
                     /locus_tag="ID725_RS04445"
                     /old_locus_tag="pgaptmp_000889"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGV7849550.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            16601..17680
                     /gene="fbaA"
                     /locus_tag="ID725_RS04450"
                     /old_locus_tag="pgaptmp_000890"
     CDS             16601..17680
                     /gene="fbaA"
                     /locus_tag="ID725_RS04450"
                     /old_locus_tag="pgaptmp_000890"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGV7849551.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(17744..17842)
                     /locus_tag="ID725_RS04455"
                     /old_locus_tag="pgaptmp_000891"
                     /pseudo
     CDS             complement(17744..17842)
                     /locus_tag="ID725_RS04455"
                     /old_locus_tag="pgaptmp_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18038..18898
                     /gene="mscS"
                     /locus_tag="ID725_RS04460"
                     /old_locus_tag="pgaptmp_000892"
     CDS             18038..18898
                     /gene="mscS"
                     /locus_tag="ID725_RS04460"
                     /old_locus_tag="pgaptmp_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGV7849552.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            19037..19672
                     /gene="argO"
                     /locus_tag="ID725_RS04465"
                     /old_locus_tag="pgaptmp_000893"
     CDS             19037..19672
                     /gene="argO"
                     /locus_tag="ID725_RS04465"
                     /old_locus_tag="pgaptmp_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGV7849553.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            19765..20505
                     /gene="yggE"
                     /locus_tag="ID725_RS04470"
                     /old_locus_tag="pgaptmp_000894"
     CDS             19765..20505
                     /gene="yggE"
                     /locus_tag="ID725_RS04470"
                     /old_locus_tag="pgaptmp_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGV7849554.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            20672..21568
                     /gene="ygfI"
                     /locus_tag="ID725_RS04475"
                     /old_locus_tag="pgaptmp_000895"
                     /pseudo
     CDS             20672..21568
                     /gene="ygfI"
                     /locus_tag="ID725_RS04475"
                     /old_locus_tag="pgaptmp_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(21565..23043)
                     /gene="scpC"
                     /locus_tag="ID725_RS04480"
                     /old_locus_tag="pgaptmp_000896"
     CDS             complement(21565..23043)
                     /gene="scpC"
                     /locus_tag="ID725_RS04480"
                     /old_locus_tag="pgaptmp_000896"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGV7849555.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(23067..23852)
                     /gene="scpB"
                     /locus_tag="ID725_RS04485"
                     /old_locus_tag="pgaptmp_000897"
     CDS             complement(23067..23852)
                     /gene="scpB"
                     /locus_tag="ID725_RS04485"
                     /old_locus_tag="pgaptmp_000897"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGV7849556.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(23863..24858)
                     /gene="meaB"
                     /locus_tag="ID725_RS04490"
                     /old_locus_tag="pgaptmp_000898"
     CDS             complement(23863..24858)
                     /gene="meaB"
                     /locus_tag="ID725_RS04490"
                     /old_locus_tag="pgaptmp_000898"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGV7849557.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(24851..26995)
                     /gene="scpA"
                     /locus_tag="ID725_RS04495"
                     /old_locus_tag="pgaptmp_000899"
     CDS             complement(24851..26995)
                     /gene="scpA"
                     /locus_tag="ID725_RS04495"
                     /old_locus_tag="pgaptmp_000899"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGV7849558.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(27199..28092)
                     /gene="argP"
                     /locus_tag="ID725_RS04500"
                     /old_locus_tag="pgaptmp_000900"
     CDS             complement(27199..28092)
                     /gene="argP"
                     /locus_tag="ID725_RS04500"
                     /old_locus_tag="pgaptmp_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGV7849559.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            28475..29134
                     /gene="rpiA"
                     /locus_tag="ID725_RS04505"
                     /old_locus_tag="pgaptmp_000901"
     CDS             28475..29134
                     /gene="rpiA"
                     /locus_tag="ID725_RS04505"
                     /old_locus_tag="pgaptmp_000901"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGV7849560.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29389..30621
                     /gene="serA"
                     /locus_tag="ID725_RS04510"
                     /old_locus_tag="pgaptmp_000902"
     CDS             29389..30621
                     /gene="serA"
                     /locus_tag="ID725_RS04510"
                     /old_locus_tag="pgaptmp_000902"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGV7849561.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            30850..30909
                     /gene="ibsC"
                     /locus_tag="ID725_RS04515"
                     /old_locus_tag="pgaptmp_000903"
     CDS             30850..30909
                     /gene="ibsC"
                     /locus_tag="ID725_RS04515"
                     /old_locus_tag="pgaptmp_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGV7849562.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            31243..31302
                     /locus_tag="ID725_RS04520"
                     /old_locus_tag="pgaptmp_000904"
     CDS             31243..31302
                     /locus_tag="ID725_RS04520"
                     /old_locus_tag="pgaptmp_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGV7849563.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(31403..32005)
                     /gene="fau"
                     /locus_tag="ID725_RS04525"
                     /old_locus_tag="pgaptmp_000905"
     CDS             complement(31403..32005)
                     /gene="fau"
                     /locus_tag="ID725_RS04525"
                     /old_locus_tag="pgaptmp_000905"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGV7849564.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(32026..32209)
                     /gene="ssrS"
                     /locus_tag="ID725_RS04530"
                     /old_locus_tag="pgaptmp_000906"
     ncRNA           complement(32026..32209)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ID725_RS04530"
                     /old_locus_tag="pgaptmp_000906"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(32251..32580)
                     /gene="zapA"
                     /locus_tag="ID725_RS04535"
                     /old_locus_tag="pgaptmp_000907"
     CDS             complement(32251..32580)
                     /gene="zapA"
                     /locus_tag="ID725_RS04535"
                     /old_locus_tag="pgaptmp_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGV7849565.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            32748..33326
                     /gene="ygfB"
                     /locus_tag="ID725_RS04540"
                     /old_locus_tag="pgaptmp_000908"
     CDS             32748..33326
                     /gene="ygfB"
                     /locus_tag="ID725_RS04540"
                     /old_locus_tag="pgaptmp_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGV7849566.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            33352..34677
                     /gene="pepP"
                     /locus_tag="ID725_RS04545"
                     /old_locus_tag="pgaptmp_000909"
     CDS             33352..34677
                     /gene="pepP"
                     /locus_tag="ID725_RS04545"
                     /old_locus_tag="pgaptmp_000909"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGV7849567.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            34674..35852
                     /gene="ubiH"
                     /locus_tag="ID725_RS04550"
                     /old_locus_tag="pgaptmp_000910"
                     /gene_synonym="visB"
     CDS             34674..35852
                     /gene="ubiH"
                     /locus_tag="ID725_RS04550"
                     /old_locus_tag="pgaptmp_000910"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGV7849568.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLAHCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            35876..37078
                     /gene="ubiI"
                     /locus_tag="ID725_RS04555"
                     /old_locus_tag="pgaptmp_000911"
                     /gene_synonym="visC"
     CDS             35876..37078
                     /gene="ubiI"
                     /locus_tag="ID725_RS04555"
                     /old_locus_tag="pgaptmp_000911"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGV7849569.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(37152..37235)
                     /locus_tag="ID725_RS04560"
                     /old_locus_tag="pgaptmp_000912"
     CDS             complement(37152..37235)
                     /locus_tag="ID725_RS04560"
                     /old_locus_tag="pgaptmp_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849570.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            37526..38620
                     /gene="gcvT"
                     /locus_tag="ID725_RS04565"
                     /old_locus_tag="pgaptmp_000913"
     CDS             37526..38620
                     /gene="gcvT"
                     /locus_tag="ID725_RS04565"
                     /old_locus_tag="pgaptmp_000913"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGV7849571.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            38644..39033
                     /gene="gcvH"
                     /locus_tag="ID725_RS04570"
                     /old_locus_tag="pgaptmp_000914"
     CDS             38644..39033
                     /gene="gcvH"
                     /locus_tag="ID725_RS04570"
                     /old_locus_tag="pgaptmp_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGV7849572.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            39151..42024
                     /gene="gcvP"
                     /locus_tag="ID725_RS04575"
                     /old_locus_tag="pgaptmp_000915"
     CDS             39151..42024
                     /gene="gcvP"
                     /locus_tag="ID725_RS04575"
                     /old_locus_tag="pgaptmp_000915"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGV7849573.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(42190..43623)
                     /gene="bglA"
                     /locus_tag="ID725_RS04580"
                     /old_locus_tag="pgaptmp_000916"
     CDS             complement(42190..43623)
                     /gene="bglA"
                     /locus_tag="ID725_RS04580"
                     /old_locus_tag="pgaptmp_000916"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGV7849574.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            43668..43979
                     /gene="yqfB"
                     /locus_tag="ID725_RS04585"
                     /old_locus_tag="pgaptmp_000917"
     CDS             43668..43979
                     /gene="yqfB"
                     /locus_tag="ID725_RS04585"
                     /old_locus_tag="pgaptmp_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGV7849575.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            44143..44802
                     /gene="yqfA"
                     /locus_tag="ID725_RS04590"
                     /old_locus_tag="pgaptmp_000918"
     CDS             44143..44802
                     /gene="yqfA"
                     /locus_tag="ID725_RS04590"
                     /old_locus_tag="pgaptmp_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGV7849576.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(44879..45859)
                     /gene="ygfZ"
                     /locus_tag="ID725_RS04595"
                     /old_locus_tag="pgaptmp_000919"
     CDS             complement(44879..45859)
                     /gene="ygfZ"
                     /locus_tag="ID725_RS04595"
                     /old_locus_tag="pgaptmp_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGV7849577.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            46102..46368
                     /gene="sdhE"
                     /locus_tag="ID725_RS04600"
                     /old_locus_tag="pgaptmp_000920"
     CDS             46102..46368
                     /gene="sdhE"
                     /locus_tag="ID725_RS04600"
                     /old_locus_tag="pgaptmp_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGV7849578.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            46349..46756
                     /gene="ygfX"
                     /locus_tag="ID725_RS04605"
                     /old_locus_tag="pgaptmp_000921"
     CDS             46349..46756
                     /gene="ygfX"
                     /locus_tag="ID725_RS04605"
                     /old_locus_tag="pgaptmp_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGV7849579.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(46796..47317)
                     /gene="fldB"
                     /locus_tag="ID725_RS04610"
                     /old_locus_tag="pgaptmp_000922"
     CDS             complement(46796..47317)
                     /gene="fldB"
                     /locus_tag="ID725_RS04610"
                     /old_locus_tag="pgaptmp_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGV7849580.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            47429..48325
                     /gene="xerD"
                     /locus_tag="ID725_RS04615"
                     /old_locus_tag="pgaptmp_000923"
     CDS             47429..48325
                     /gene="xerD"
                     /locus_tag="ID725_RS04615"
                     /old_locus_tag="pgaptmp_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGV7849581.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            48350..49060
                     /gene="dsbC"
                     /locus_tag="ID725_RS04620"
                     /old_locus_tag="pgaptmp_000924"
     CDS             48350..49060
                     /gene="dsbC"
                     /locus_tag="ID725_RS04620"
                     /old_locus_tag="pgaptmp_000924"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGV7849582.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            49066..50799
                     /gene="recJ"
                     /locus_tag="ID725_RS04625"
                     /old_locus_tag="pgaptmp_000925"
     CDS             49066..50799
                     /gene="recJ"
                     /locus_tag="ID725_RS04625"
                     /old_locus_tag="pgaptmp_000925"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGV7849583.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            50890..51988
                     /gene="prfB"
                     /locus_tag="ID725_RS04630"
                     /old_locus_tag="pgaptmp_000926"
     CDS             join(50890..50964,50966..51988)
                     /gene="prfB"
                     /locus_tag="ID725_RS04630"
                     /old_locus_tag="pgaptmp_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGV7849584.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            51998..53515
                     /gene="lysS"
                     /locus_tag="ID725_RS04635"
                     /old_locus_tag="pgaptmp_000927"
     CDS             51998..53515
                     /gene="lysS"
                     /locus_tag="ID725_RS04635"
                     /old_locus_tag="pgaptmp_000927"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGV7849585.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(53558..54106)
                     /gene="idi"
                     /locus_tag="ID725_RS04640"
                     /old_locus_tag="pgaptmp_000928"
     CDS             complement(53558..54106)
                     /gene="idi"
                     /locus_tag="ID725_RS04640"
                     /old_locus_tag="pgaptmp_000928"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGV7849586.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            54161..54232
                     /locus_tag="ID725_RS04645"
                     /old_locus_tag="pgaptmp_000929"
     CDS             54161..54232
                     /locus_tag="ID725_RS04645"
                     /old_locus_tag="pgaptmp_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849587.1"
                     /translation="MINQASVYRQPPVLSGCRQVKTI"
     gene            complement(54229..54354)
                     /gene="yqfG"
                     /locus_tag="ID725_RS04650"
                     /old_locus_tag="pgaptmp_000930"
     CDS             complement(54229..54354)
                     /gene="yqfG"
                     /locus_tag="ID725_RS04650"
                     /old_locus_tag="pgaptmp_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGV7849588.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(54356..55804)
                     /gene="uacT"
                     /locus_tag="ID725_RS04655"
                     /old_locus_tag="pgaptmp_000931"
     CDS             complement(54356..55804)
                     /gene="uacT"
                     /locus_tag="ID725_RS04655"
                     /old_locus_tag="pgaptmp_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGV7849589.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            56240..58159
                     /gene="ygfT"
                     /locus_tag="ID725_RS04660"
                     /old_locus_tag="pgaptmp_000932"
     CDS             56240..58159
                     /gene="ygfT"
                     /locus_tag="ID725_RS04660"
                     /old_locus_tag="pgaptmp_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGV7849590.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            58159..58647
                     /gene="ygfS"
                     /locus_tag="ID725_RS04665"
                     /old_locus_tag="pgaptmp_000933"
                     /pseudo
     CDS             58159..58647
                     /gene="ygfS"
                     /locus_tag="ID725_RS04665"
                     /old_locus_tag="pgaptmp_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(58683..60050)
                     /gene="ghxQ"
                     /locus_tag="ID725_RS04670"
                     /old_locus_tag="pgaptmp_000934"
                     /gene_synonym="ygfQ"
     CDS             complement(58683..60050)
                     /gene="ghxQ"
                     /locus_tag="ID725_RS04670"
                     /old_locus_tag="pgaptmp_000934"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGV7849591.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(60086..61402)
                     /gene="guaD"
                     /locus_tag="ID725_RS04675"
                     /old_locus_tag="pgaptmp_000935"
     CDS             complement(60086..61402)
                     /gene="guaD"
                     /locus_tag="ID725_RS04675"
                     /old_locus_tag="pgaptmp_000935"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGV7849592.1"
                     /translation="MSGEHTLKAVRGSFIDVARTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(61420..62820)
                     /gene="xanQ"
                     /locus_tag="ID725_RS04680"
                     /old_locus_tag="pgaptmp_000936"
     CDS             complement(61420..62820)
                     /gene="xanQ"
                     /locus_tag="ID725_RS04680"
                     /old_locus_tag="pgaptmp_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGV7849593.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(62985..65855)
                     /gene="xdhD"
                     /locus_tag="ID725_RS04685"
                     /old_locus_tag="pgaptmp_000937"
     CDS             complement(62985..65855)
                     /gene="xdhD"
                     /locus_tag="ID725_RS04685"
                     /old_locus_tag="pgaptmp_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGV7849594.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(65852..66631)
                     /gene="ygfM"
                     /locus_tag="ID725_RS04690"
                     /old_locus_tag="pgaptmp_000938"
     CDS             complement(65852..66631)
                     /gene="ygfM"
                     /locus_tag="ID725_RS04690"
                     /old_locus_tag="pgaptmp_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGV7849595.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(66681..68009)
                     /gene="ssnA"
                     /locus_tag="ID725_RS04695"
                     /old_locus_tag="pgaptmp_000939"
     CDS             complement(66681..68009)
                     /gene="ssnA"
                     /locus_tag="ID725_RS04695"
                     /old_locus_tag="pgaptmp_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGV7849596.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(68012..71110)
                     /gene="ygfK"
                     /locus_tag="ID725_RS04700"
                     /old_locus_tag="pgaptmp_000940"
     CDS             complement(68012..71110)
                     /gene="ygfK"
                     /locus_tag="ID725_RS04700"
                     /old_locus_tag="pgaptmp_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGV7849597.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(71432..72010)
                     /gene="mocA"
                     /locus_tag="ID725_RS04705"
                     /old_locus_tag="pgaptmp_000941"
     CDS             complement(71432..72010)
                     /gene="mocA"
                     /locus_tag="ID725_RS04705"
                     /old_locus_tag="pgaptmp_000941"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGV7849598.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            72113..72883
                     /gene="yqeC"
                     /locus_tag="ID725_RS04710"
                     /old_locus_tag="pgaptmp_000942"
     CDS             72113..72883
                     /gene="yqeC"
                     /locus_tag="ID725_RS04710"
                     /old_locus_tag="pgaptmp_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGV7849599.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            72931..74556
                     /gene="yqeB"
                     /locus_tag="ID725_RS04715"
                     /old_locus_tag="pgaptmp_000943"
     CDS             72931..74556
                     /gene="yqeB"
                     /locus_tag="ID725_RS04715"
                     /old_locus_tag="pgaptmp_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGV7849600.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(74597..75529)
                     /gene="arcC"
                     /locus_tag="ID725_RS04720"
                     /old_locus_tag="pgaptmp_000944"
     CDS             complement(74597..75529)
                     /gene="arcC"
                     /locus_tag="ID725_RS04720"
                     /old_locus_tag="pgaptmp_000944"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGV7849601.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(75577..76962)
                     /gene="hyuA"
                     /locus_tag="ID725_RS04725"
                     /old_locus_tag="pgaptmp_000945"
     CDS             complement(75577..76962)
                     /gene="hyuA"
                     /locus_tag="ID725_RS04725"
                     /old_locus_tag="pgaptmp_000945"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGV7849602.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(77015..78226)
                     /gene="ygeY"
                     /locus_tag="ID725_RS04730"
                     /old_locus_tag="pgaptmp_000946"
     CDS             complement(77015..78226)
                     /gene="ygeY"
                     /locus_tag="ID725_RS04730"
                     /old_locus_tag="pgaptmp_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGV7849603.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(78284..79480)
                     /gene="dpaL"
                     /locus_tag="ID725_RS04735"
                     /old_locus_tag="pgaptmp_000947"
     CDS             complement(78284..79480)
                     /gene="dpaL"
                     /locus_tag="ID725_RS04735"
                     /old_locus_tag="pgaptmp_000947"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGV7849604.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(79538..80725)
                     /gene="ygeW"
                     /locus_tag="ID725_RS04740"
                     /old_locus_tag="pgaptmp_000948"
     CDS             complement(79538..80725)
                     /gene="ygeW"
                     /locus_tag="ID725_RS04740"
                     /old_locus_tag="pgaptmp_000948"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGV7849605.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            81204..82982
                     /gene="ygeV"
                     /locus_tag="ID725_RS04745"
                     /old_locus_tag="pgaptmp_000949"
     CDS             81204..82982
                     /gene="ygeV"
                     /locus_tag="ID725_RS04745"
                     /old_locus_tag="pgaptmp_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGV7849606.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(83022..83501)
                     /gene="xdhC"
                     /locus_tag="ID725_RS04750"
                     /old_locus_tag="pgaptmp_000950"
     CDS             complement(83022..83501)
                     /gene="xdhC"
                     /locus_tag="ID725_RS04750"
                     /old_locus_tag="pgaptmp_000950"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGV7849607.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(83498..84376)
                     /gene="xdhB"
                     /locus_tag="ID725_RS04755"
                     /old_locus_tag="pgaptmp_000951"
     CDS             complement(83498..84376)
                     /gene="xdhB"
                     /locus_tag="ID725_RS04755"
                     /old_locus_tag="pgaptmp_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGV7849608.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(84387..86684)
                     /gene="xdhA"
                     /locus_tag="ID725_RS04760"
                     /old_locus_tag="pgaptmp_000952"
     CDS             complement(84387..86684)
                     /gene="xdhA"
                     /locus_tag="ID725_RS04760"
                     /old_locus_tag="pgaptmp_000952"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGV7849609.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            87100..87855
                     /gene="actS"
                     /locus_tag="ID725_RS04765"
                     /old_locus_tag="pgaptmp_000953"
     CDS             87100..87855
                     /gene="actS"
                     /locus_tag="ID725_RS04765"
                     /old_locus_tag="pgaptmp_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGV7849610.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            87934..88007
                     /locus_tag="ID725_RS04770"
                     /old_locus_tag="pgaptmp_000954"
     tRNA            87934..88007
                     /locus_tag="ID725_RS04770"
                     /old_locus_tag="pgaptmp_000954"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87966..87968,aa:Gly,seq:ccc)
     gene            88241..89846
                     /locus_tag="ID725_RS04775"
                     /old_locus_tag="pgaptmp_000955"
                     /pseudo
     CDS             88241..89846
                     /locus_tag="ID725_RS04775"
                     /old_locus_tag="pgaptmp_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
     gene            89913..90092
                     /locus_tag="ID725_RS04780"
                     /old_locus_tag="pgaptmp_000956"
     CDS             89913..90092
                     /locus_tag="ID725_RS04780"
                     /old_locus_tag="pgaptmp_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HGV7849611.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT        21950 a        22370 c        23714 g        22092 t
ORIGIN      
        1 atctccctac aatcgctgca ttaattaaat gcagagggaa cggttatgaa aaaatggaaa
       61 gtacgcagcg cgttggtggc attaatcgtg ctgctggccg gttgttccag taatgcgcag
      121 tataatagca gtgcatcggg caatgtcggc accgcctggg gtggggatgt gcactcaacg
      181 gttcagggcg tatctgctga gcgagcctgg cgcgatccgg cggagatgat cgttattagt
      241 tattcgacta atgttccttc cggctacgat cgcgtttata gcattcgtat taacgaactg
      301 gaatatgcta ttcgcgatgg taattttaat tctctaccta ttacgcgtgt ttatgattca
      361 tctaataatg aaccacgcta tattgtccat gccagagtcg gaatgaatta tcaattatat
      421 gtccgtaatt acagtcgtaa caccaactac gagatcgttg ctacggttga tggcctggat
      481 gtgctgaacg gcaagcaagg ttcgctgaat aacaacggct atattgtgaa tgccggagat
      541 tcgttagcga ttaaaggatt ccgcaaagat aaacataccg aagcggcttt tcagttcgcc
      601 aacgttgctg attcctacgc agccaattcg gcgcaaggcg atgtgcgtaa taccggtgta
      661 atcggctttg ccgcctttga attacaaggc caggcgcaaa acgcgctgcc accgtgctct
      721 ggacaagcct ttccggcaga taacaatggc tatgcgccgc caccgtgtcg gaaatagcaa
      781 cctcgcttga tgcctcgccc atttaaggcg ataattagca caaacttgtg tttcgcaaat
      841 ccaacccttc ctcgtcgggc ctaacgacgc ggaagggttt ttttatatcg actttgtaat
      901 aggagtccat ccatgagcac cttaggtcat caatacgata actcactggt ttccaacgcc
      961 tttggttttt tgcgcctgcc gatgaacttc cagccgtatg acagcgatgc tgactgggtg
     1021 attactggtg tgccgttcga tatggccact tctggtcgtg cggggggacg ccacggtccg
     1081 gcagctatcc gtcaggtttc gacgaatctg gcctgggaac acaaccgctt cccgtggaat
     1141 ttcgacatgc gtgagcgtct gaacgtcgtg gactgtggtg atctggtata tgctttcggc
     1201 gatgcccgtg agatgagcga gaagctgcag gcgcacgccg agaagctgct ggctgccggt
     1261 aagcgtatgc tctccttcgg tggtgaccac tttgttacgc tgccgctgct gcgtgctcat
     1321 gcgaagcatt tcggcaaaat ggcgctggta cactttgacg cccacaccga tacctacgcg
     1381 aacggttgtg aatttgacca cggcaccatg ttctataccg cgccgaaaga aggtctgatc
     1441 gacccgaatc attccgtgca gattggtatt cgtaccgaat ttgataaaga caacggcttt
     1501 accgtgctgg acgcctgcca ggtgaacgat cgcagcgtgg atgacgttat cgcccaggtg
     1561 aaacaaattg tgggtgatat gccggtttac ctgacctttg atatcgactg cctggatcct
     1621 gcatttgcgc cgggaaccgg tacgccggta attggcggtc tgacctccga tcgcgctatt
     1681 aaactggtac gcggcctgaa agatctcaac atcgttggga tggacgtagt ggaagtggct
     1741 ccggcatacg atcagtcgga aatcaccact ctggcagcgg caacgctggc gctggaaatg
     1801 ctgtatattc aggcggcgaa aaagggcgag taagcaccag atgcgatgcg cacgggtaaa
     1861 acgtgccatt aatgtcggat gcggcgtgaa cgccttatcc gacctacgtt cggcacccgt
     1921 aggccggata agatgcgcca gcatcgcatc cggcaatgcg cacaaggtaa caaatgtgcc
     1981 attcatgtca gatgcggcgt gaacgcctta tctgacctac gttcgacacc accaggcttt
     2041 acttaatccc atccgcgctc atgcgatcgc gaatatgctg ggcgcgttcg gcggatgcag
     2101 gatggtcgtc aaacattgag ctttggcgac cttcttccag ttttgccagt ttttcaaagc
     2161 tggtggcaag acctgccggg ctgatgccgc gttggcgcag aagatcgtaa gaataatcat
     2221 cggcttctgc ttcctggcgc tgggagaatt gcgaattaac taatttctcg cccagatcac
     2281 cgagttgtga ttgagataaa ctgccgacaa tcccgcccgc cgaggccgca gctactcgta
     2341 cggcatttgt accaagtgcc acctgcattc cttttttcac atggcctaat gccacgtgcc
     2401 ccatttcgtg accgatcacc gcttcgactt cgttatccgt catcatatcc atcagcccgc
     2461 tatagacacg gatacagccg ttagccattg caaaggcgtt cacatccttc gccatataca
     2521 ctttgtaatt taccggctga ccgttgatat tgttgcctag cgcattggca atagttgtca
     2581 gacgtttagc gtattcgcta ttggctggcg caatcgtcgc cttgctgtcc atctcctgac
     2641 atgcctgatc gctcagggtt ttcacctgcg catcactcaa actgtaagcc tgaaaagctt
     2701 ccgctcctga tgagagcagt ccgttggagt ccatattctg gcaaccggtc agtaccgttg
     2761 ccacgctcat tgctaccaat aaggcgcgaa ttttcatgtt ttttcttccg cacaaggtca
     2821 ggattattct aaaaatgcgc cgtttgcagg tgaaccgacg ctcagtctca gtataaggaa
     2881 aagcgcagcg gacgggcgag tagattgcgc aacatgcgag catgatccag agatttctga
     2941 agcggcaaaa ggatgttcca tgtacatgac gcgcggcttg cggtaaattg ttggcaaatt
     3001 ttccggcgta gcccaaaacg cgctgtcgtc aagtcgttaa gggcgtgccc ttcatcatcc
     3061 gatctggagt caaaatgtcc tcacgtaaag agcttgccaa tgctattcgt gcgctgagca
     3121 tggacgcagt acagaaagcc aaatccggtc acccgggtgc ccctatgggt atggctgaca
     3181 ttgccgaagt cctgtggcgt gatttcctga aacataaccc gcagaatccg tcctgggctg
     3241 accgtgaccg cttcgtgctg tccaacggcc acggctccat gctgatctac agcctgctgc
     3301 acctcaccgg ttacgatctg ccgatggaag aactgaaaaa cttccgtcag ctgcactcta
     3361 aaactccggg ccacccggaa gtgggttaca ccgctggtgt ggaaaccacc accggtccgc
     3421 tggggcaggg tattgccaac gcagtaggta tggcgattgc agaaaaaacg ctggcggcgc
     3481 agtttaaccg tccgggccac gacattgtcg accactacac ctacgccttc atgggcgacg
     3541 gctgcatgat ggaaggcatc tcccacgaag tttgctctct ggcgggtacg ctgaagctgg
     3601 gtaaactgat tgcgttctac gatgacaacg gtatttctat cgatggtcac gttgaaggct
     3661 ggttcaccga cgacaccgca atgcgtttcg aagcttacgg ctggcacgtt attcgcgaca
     3721 tcgacggtca tgacgcagca tccatcaaac gcgcagtaga agaagcgcgc gcagtgactg
     3781 acaaaccgtc cctgctgatg tgcaaaacca tcatcggttt cggttccccg aacaaagccg
     3841 gtactcacga ctcccacggt gcgccgctgg gtgatgcaga aatcgctctg acccgcgaac
     3901 agctgggctg gaaatacgca ccgttcgaaa tcccgtctga aatctatgcg cagtgggatg
     3961 cgaaagaagc aggccaggcg aaagaatctg catggaatga gaagtttgcg gcttacgcga
     4021 aagcttatcc gcaggaagcg gctgaattta cccgccgtat gaaaggcgaa atgccgtctg
     4081 acttcgacgc caaagcgaaa gagtttatcg ctaaactgca ggctaatccg gcgaaaatcg
     4141 ccagccgtaa agcgtctcag aatgctatcg aagcgttcgg cccgctgttg ccggaattcc
     4201 tcggcggctc cgctgacctg gcaccgtcta acctgaccct gtggtctggt tctaaagcaa
     4261 tcaacgaaga tgctgcgggt aactacatcc actacggtgt tcgcgagttc ggtatgaccg
     4321 cgattgctaa cggtatctcc ctgcacggtg gcttcctgcc gtacacctcc accttcctga
     4381 tgtttgtcga atacgcacgt aacgccgtac gtatggctgc gctgatgaaa cagcgtcagg
     4441 ttatggttta cacccacgac tccatcggtc tgggcgaaga tggcccgact caccagccgg
     4501 ttgagcaggt tgcttctctg cgcgtgaccc cgaacatgtc tacatggcgt ccgtgtgacc
     4561 aggttgaatc cgcggtcgcg tggaaatacg gtgttgagcg tcaggacggt ccgaccgcgc
     4621 ttatcctctc ccgtcagaac ctggcgcagc aggaacgtac tgaagagcaa ctggcaaaca
     4681 tcgcgcgcgg tggttatgtg ctgaaagact gtgctggtca gccggaactg atcttcatcg
     4741 cgaccggttc ggaagttgaa ctggctgttg ccgcctacga aaaactgact gccgaaggcg
     4801 tgaaggcgcg cgtggtttcc atgccgtcta ccgatgcatt cgacaagcag gatgcggctt
     4861 accgtgaatc cgtactgccg aaagcggtta ctgcacgcgt tgcggtagaa gcgggtattg
     4921 ctgactactg gtacaagtat gttggcctga atggcgctat catcggtatg accaccttcg
     4981 gtgagtcagc tccggcagag ttgctgttcg aagagttcgg cttcaccgtc gacaacgtcg
     5041 ttgcgaaagc aaaagaactg ctgtaactag catttcgggt aaaaaggtcg cttcggcgac
     5101 cttttttatt accttgatat gtccgtttgc ggacaagcaa tagataaagc gtgttgtaga
     5161 tcgcaaatat ttatatgcaa taaatatcaa ttatgtaata tgcatcacga tatgcgtgtt
     5221 gacatttgtt gttatatcta taactcaatg ttatataaga aattaataat tcactgtttt
     5281 caaaacaccg gtttccctgc tcaattgctt tcattaaacc gcacgagtat tttattttta
     5341 ccgccggacg gtaccctaaa aataaaataa ggataattta tgcggcttag tgactatttt
     5401 ccagaatcat caatctcagt tatacattca gcaaaagatt ggcaggaagc tatcgatttc
     5461 tcgatggtat cattgctgga taaaaactat atcagcgaga attacattca ggcaattaaa
     5521 gattccacca ttaacaatgg cccttattat attctcgcac caggcgtggc aatgcctcat
     5581 gcgcgaccgg aatgtggggc gcttaaaacc gggatgtctt tgacattact tgaacaaggt
     5641 gtttattttc cggggaatga cgaaccaatt aaattactca tcggactctc tgctgccgat
     5701 gccgattcgc acattggcgc tattcaggcg ttaagtgaat tactgtgcga agaagaaata
     5761 ctcgaacaac tcttaacagc atcatcagaa aaacaattag cggacattat cagccgcgga
     5821 taattactct ctcctctaca ggataatatt atggaaaaca agtctgctcg tgcaaaggtc
     5881 caggcttttg ggggcttttt gactgcaatg gtcatcccca atattggtgc ttttattgcc
     5941 tggggtttta ttactgcgtt atttattccc accggttggc tgcctaacga acatttcgcc
     6001 aaaattgtcg gcccgatgat tacctattta ttgcccgtga tgattggttc taccggtggt
     6061 catctggtcg gcggtaaacg cggcgcggtc atgggcggaa taggtactat tggtgtgatc
     6121 gtcggcgcag agatcccgat gttccttggc tcaatgatta tggggccgct cggtggtctg
     6181 gtcataaaat atgttgataa ggcactggaa aaacgcatac ctgccggttt tgagatggtt
     6241 atcaataact tctcattagg tatcgcgggg atgctccttt gcctgctggg ttttgaagtg
     6301 atcggcccgg cggtgttaat tgccaatact ttcgtcaaag agtgtattga ggcgctggta
     6361 catgcgggtt atctgcctct gctgtcagtc atcaatgaac cggcgaaagt gcttttcctt
     6421 aataatgcga tcgatcaggg cgtctattat ccgctgggaa tgcaacaggc ttcggttaac
     6481 ggtaaatcca tcttctttat ggtggcctct aacccaggtc cgggcctggg gctgctgctg
     6541 gcgtttacct tgtttggtaa agggatgagt aaacgttctg cgcccggggc gatgattatt
     6601 cacttcctcg gtgggatcca cgaactgtat ttcccatatg tgctgatgaa gccgctgact
     6661 attattgcca tgattgcggg cggtatgtct ggcacctgga tgtttaactt actggacggt
     6721 ggtctggtgg ctggcccaag tccggggtct atctttgctt acctggcgct gacgccgaaa
     6781 ggttcgttcc tggcgacaat tgccggtgtt acggtaggta ccctggtgtc ctttgctatc
     6841 acttcgctga tactgaagat ggaaaaaacg gtggaaacgg agagcgaaga tgagtttgct
     6901 cagtcagcca atgcggttaa ggcgatgaaa caagagggtg cattctcgtt aagcagggtt
     6961 aagcgtatcg cctttgtttg cgatgcgggg atgggctcca gtgcgatggg cgcgaccacc
     7021 ttccgtaaac gcctggaaaa agcggggctg gcaattgaag taaaacatta cgccatagaa
     7081 aacgtgcctg cggatgcgga tatcgtcgtt actcatgcca gtctggaagg gcgcgtgaaa
     7141 cgtgtgacgg ataaaccact gatattgatt aataactata ttggcgatcc aaaactcgac
     7201 actttattta atcaattaac cgccgaacat aaacactgat tacagaggta aaaatgaaaa
     7261 ccaaagttgc tgctatttat ggcaagcggg atgtccgtct gcgcgaattt gaactgccag
     7321 aaattaccga taatgaatta ctggtgagtg tgatttctga cagcgtctgt ttatccacct
     7381 ggaaagcggc gttactcggt agtgaacata aacgcgtacc cgacgattta gaaaatcatc
     7441 cggtcattac cgggcatgaa tgtgccgggg ttattgtcga agtgggtaaa aatctcactg
     7501 gcaaatataa aaaaggccag cgttttgtat tgcaaccggc gatggggtta ccaagcggat
     7561 attcagcggg ctacagctac gaatattttg gcggcaatgc cacttatatg attattcccg
     7621 aaatcgccat taatttgggc tgcgtattac cgtatcacgg ctcttatttt gctgcggcgt
     7681 cgctggcaga gcctatgtgc tgcattattg gcgcttatca tgccaattat cacaccacgc
     7741 aatatgttta tgagcatcgc atgggcgtca aacctggcgg caatattgca ctgctggcgt
     7801 gtgcaggtcc gatgggcatt ggcgctatcg attacgccat taacggcggc atacaaccgt
     7861 cgcgggtggt ggtggtcgat atcgacgaca aacgtctggc gcaggtacag aagctgctgc
     7921 cggtggaact ggcggccagt aaaggcattg agctggtgta tgtgaatacc aaagggatga
     7981 gcgatcctgt ccagatgctg cgggcgctga caggagatgc cgggttcgat gacatttttg
     8041 tttatgcggc ggtgtctgct gtcgttgaga tggctgatga attactggcg gaagatggct
     8101 gtctgaactt ctttgccggg ccgacggata aaaacttcaa agtgccgttt aatttctaca
     8161 acgttcatta caacagcacg cacgtcgtcg gtacatccgg cggttcaacg gacgacatga
     8221 aagaggcgat tgcccttagc gccacagggc agttacagcc ctcgtttatg gtgacccata
     8281 tcggtggcct ggatgcggtg ccagaaaccg tgctcaatct gccggatatc cctggcggta
     8341 aaaaactcat ttataacggc gtgaccatgc cgctcactgc cattgccgat tttgccgaaa
     8401 aaggcaaaac cgatccgctg tttaaagagt tggcgcggct ggttgaggaa acgcacggca
     8461 tctggaatga acaggccgag aaatatctgc tggcacaatt tggcgttgat atcggggagg
     8521 ccgcgcaatg atgtccctgg cgtggccatt atttcgcgtt acggaacagg cagcgttggc
     8581 tgcctggccg caaaccggat gtggcgacaa aaataaaatt gatggcctgg cggtcaccgc
     8641 gatgcgccag gcattaaacg acgtcgcttt tcgtggacga gtggttatcg gcgaaggaga
     8701 aattgaccat gcgccgatgc tgtggattgg cgaagaggtg ggcaaaggcg atgggccaga
     8761 agtcgatatc gcggttgatc ccattgaagg cacgcggatg gtggcgatgg ggcagagcaa
     8821 tgcgctggcg gtcatggctt tcgcccctcg agatagcctg ttacatgcgc ccgatatgta
     8881 tatgaaaaag ctggttgtta atcgactggc tgccggggcg attgatctgt ctttgcctct
     8941 ggcggacaac ttgcgtgacg tggcgcgggc gttaggtaaa ccgctggaca agctgcgcat
     9001 ggttacgctg gataaaccgc gtctgagtgc cgcgattgaa gaagcaacac agctgggcgt
     9061 gaaggttttt gccctgccgg atggcgatgt cgccgccagc gtgctgacct gctggcagga
     9121 taatccttac gatgtgatgt acaccatcgg cggtgcgccg gaaggcgtga tttctgcctg
     9181 tgccgttaaa gcattaggcg ggaatatgca ggccgagctg attgactttt gtcaggccaa
     9241 aggggattac acggaaaatc ggcagattgc agagcaggag cgcaagcgtt gcaaggcaat
     9301 gggtgttgac gttaaccgtg tgtactcgct cgatgtactg gtaaggggga acgatatcct
     9361 ctttagcgct acgggcgtca cgggcggcga gttggtgaac ggtatccaac agacggcgaa
     9421 tggagtgcgg acgcagacat tactgatcgg cggcgcggac caaacgtgta atataataga
     9481 ctccctgtat tgatggtgat ctatcggaag accaatggcg acgctgtcag aagatgatgt
     9541 gcttgagcaa ctggatgcac aggacaattt attttcattt atgaaaactg cgcattctat
     9601 tttgctccag gggatacgcc agtttctgcc gtcgctgttt gtcgataacg atgaagagag
     9661 cgtcgaatat gcagtgaagc cgttactcgc ccaaagcggc ccgcttgacg atattgatgt
     9721 tgcactgcgt ttgatttatg cgctggggaa aatggataaa tggctgtacg ccgatatcac
     9781 gcatttttcc cagtactggc attacctgaa agaacaggat gaaacgctcg gatttgccga
     9841 tgacatgact tgggatttta tcagcaacgt caatagcata acctgcaatg cgacgctcta
     9901 tgacgcgttg aaagcgatga agttcgccga tttcgccgtc tggtcagagg cgcgttttag
     9961 cggaatggtc aaaacggcgc tgacgctggc agtaacgacc accttaaagg aattaacgcc
    10021 gtgaaaattg aattaacggt gaatgggctg aacgtgcaag cgcagtacca tgatgaagag
    10081 atagaacgcg tgcataaacc gttgctgcgt atgctggcgg ccttacagac ggtaaatccg
    10141 cagcgccgaa ccgtggtttt tctttgcgct ccgccgggga cgggtaaatc taccctaacc
    10201 accttctggg catatctcgc gcaacaagac ccggagctgc ccgctattca gacgctcccg
    10261 atggatggtt ttcaccatta caatagctgg ctggatgcgc atcaattgcg ccccttcaaa
    10321 ggcgcaccag agacatttga cgttgcgaaa ctggcggaaa atctgcgcca ggtcgtggaa
    10381 ggggattgta cgtggccgca gtacgatcga caaaagcatg atcctgttga agatgcgttg
    10441 cacgttaccg caccaataat catcgtcgaa ggaaactggc tattgctgga tgacgataac
    10501 tggcgccagc tagcgcaatt ttgtgatttc tcgatcttta tcaatgcgcc agctactgcg
    10561 ttgcgggaac gactggtggg gcgcaagctg gcgggagggt tatcgctggc agacgcagaa
    10621 gctttttatg accgtaccga cgggccgaat gttcgccggg tgctggaaga gagtctgcct
    10681 gcaaatttga ccttaatgat gaccgctacg ggtgaatatc gtctggtgga ttaatgttcc
    10741 tgattagacc atcttccagt cgcaccacac gagagaaatg acgccgggta aatgcggcgt
    10801 catgactgat tgctacgacg ctcgtgcctc gctggcggca cttctccagc cagctttcaa
    10861 acacgctcaa ccaattttcg tcaaaatccc gactcggctc gtccagcagc aatagtggcg
    10921 gagagagtgc ttccaggcag gcgacggcga ccatccgtcg ctgcgcacta tgcagatcca
    10981 acgggtgcgc gctggcagtg tctgctaact gacaacattg cagggcagca tgggtacgtt
    11041 gagtgatttc atccgccggg catttttgca gtttcaaacc aaatgcgatc tcgtcagcga
    11101 cggtgctgtg aaaaagttgg ttttccgctt cctgaaacag tacgccaatc tttgccgcgc
    11161 gctggcggtt tttcaggttt ttcatcgcct gttgttgcag cataaccgta ccggctgtgg
    11221 gcgtaagaag acctgccatt actcgtaaca gcgtcgattt ccctgcgcca ttgtcgccgg
    11281 tcaacgccaa ccattcacct tgtttgagtt gcagagaaat gtcgcaaaga caatccgttg
    11341 ccgcgccagg ccagcgataa gatatctgat ttaacgttaa catagtggct ggagcgctcc
    11401 gttttgcaat tgccacgctt gctggcagta gttcaggaaa ggtgtgcgat gacgttcaaa
    11461 aagaataatc agcgaaccac gttcaaacgc ccagtgttgt aagcgctgca acagcatttc
    11521 gctggcctgc ggcgtcaggc ggctaaaggc ttcatcaaga attaatagct ttggctgcat
    11581 cgcgatggcg caggcgatga ccacgcgctg tgtctctcca ccggagagtg ttgccgggtg
    11641 acgatggcgc aacggctggc actcggtcag agtcagggcg gcatcaatcc gcgccataat
    11701 ctctgcttca gcaagacaca gattttctgg cccaaaggca acttcttctt ccacgctaaa
    11761 ggtacagccg gagagctgca aatagggcga ttgctggacg agctggatgg tggcggattg
    11821 ttcattaagc gggagatgcc caatgggcgt accgaggagc gtccccgtgc ctgttatctc
    11881 tcctggcaga aagtccggat accagcccgc catcagctgt gccagcgtac ttttgccact
    11941 gccgttgtca ccaaagatgg cgaccatccc cggtttgaca tagtgaaagt cataacagaa
    12001 cggaggatgc gttgagtgtg tcgggcaata acggaactgt tctaacgtaa ccatatccac
    12061 gctccgaatt ctgtcagcat cagcaagatc atggtgtaac gcgctacgcg ttgtagggtg
    12121 ctatcggctg gtgcccacaa cgttgttcgt cggttgtgga tccgaaatgc ccgcatatcc
    12181 agcgccgcac cacggacagc aagatcgttt agcgcattgt gtgttagcgg aatagttaac
    12241 gcgggcatcg cacgtaaacg ctgataccag ccctcatcca gcggcacgcc gcgggcacgc
    12301 tgcgcttcat gaataatcgc taattgccgt tttaactgct caacgaccaa taacggcccg
    12361 gcaaacagat aagcgacgcc cggcggcagg cgagaagcaa acagagcgcg gataaaacat
    12421 tgcacgggga cgtactgcat ccacaactgt gaggttgaaa caatcgccag aatccgcaac
    12481 cagagcgtaa tagcatgcgt ccaacgctcc ggcgaacgcg gcgtgccgct tagccattct
    12541 gttagccagc caccgtgtac cagccacagc ccggctccca gcgagaacat cagccagacg
    12601 acatattttg cccgccgccg cgtggccttt agcgcaataa ggcaaaaaaa ggtcgccgcg
    12661 ctatagatcg gcaaaatggt ctgcgcgggc aggataagcg tggtgcaggc cgccaatgcc
    12721 catagtgtta atgaggtaaa tggatgcatt tagcgcacgg cagccattgc cggaaaatgg
    12781 cgcgtggtgc gaatcggcag ctggcgcagt aaaagccaga caatcaccgc tgtaagagtt
    12841 ttatcgacca ggttcgcgcc tatcaccgta atcgccaccg actccaccag gttttgcccc
    12901 atcgagtgca tccaggcaac gaacaaatcg gctccgctac cggtgacacc gccgaacagc
    12961 gcagtacgca gcggtacagc gacgaccgtt accgcaagcg taataatcac gccgctgaca
    13021 acgacttttg gcagggtgcg aaaccaaccc gctcgcgcca gccaaccggc gaccaggccg
    13081 atgaccatcg cgacgggggc aaaagcggca gcgatcgggt cggtcagtaa tccccagagt
    13141 aaattggtca gtaagccggt cagcatcccg atcaccggac caagcagtac cgcgctaata
    13201 agtgtgccga tggagtcgag aaatataggt aatttgacca tgctggccag ttgcccgccg
    13261 atcatattga tggcgataga tataacgatc aacaccagtg cctgactgga aaagtggcta
    13321 cgcgccataa gaaaacctct ctgaaagtag ttatttaacg ggggaaatat gcaactcgtc
    13381 gtaaccagaa agcgcgcaag gttggcggct aaacgccagt tcctcaaggc taccgatcac
    13441 cagttcaagc gtggtccgca cggtacagcc gggcatcata tgaccgccaa gcatcgtgcc
    13501 gtgtggatcg gaaacgcaga gatgaagatg ctcaccactc tgttccaacg tgccgtttag
    13561 tgagatcact tcgaatgtac cgttcaggag cgtggtgcct tcttgcccgg catagcgcaa
    13621 ggcgatgtca gtcaggctac cggtacagcc tgcaatccag gcggcatgaa gttgctgctg
    13681 ctgtgcaaaa gcgtgtagct gggaaaaaac ttcctgcccg gggaggaggc gcagtgcgta
    13741 aaagcgtgcg gaggaagaat atgggtaggg caatgtcatc attggagtac tccgaaactc
    13801 ggacgtatta cggataatat cgacgctgca tcagtcatat tctgatataa gtcagggttt
    13861 gtgacggcac aacgaaaaga ttttgctacg cttattcgtg attctgcaaa gtggaataaa
    13921 ttccacaggg gcggggctga aaacgtgacg taagtcagat aattgttttc tttgactgga
    13981 caatcattcc ttttattcca cgtttcgctt atcctagctg aagcgtttca gtcgattaaa
    14041 tgttcgacaa ttaaccaatc agtcgcagtt tgcgacaggt aaggtttccc cggacgattt
    14101 gctggattac tctgtctggc aaatttgctc agggaaaacc ttgcaggaga tctatgaccg
    14161 tacgcgtagc gataaatggc ttcggtcgca tcgggcgtaa tgtggttcgt gctttgtatg
    14221 aatccggacg ccgggcggaa attaccgtgg tggcaatcaa cgaactggcg gatgctgcgg
    14281 gcatggcgca tttgttgaaa tacgacacca gccatggccg ttttgcatgg gaagtacgac
    14341 aggaacgcga tcaacttttt gttggtgatg acgccatccg cgtattgcat gaacgttcac
    14401 tgcaatcgct cccctggcgt gaacttggcg ttgatgtagt cctcgactgc accggcgtat
    14461 atggctcccg cgagcatggc gaagcgcaca ttgccgccgg ggctaaaaaa gtgctctttt
    14521 cacatcctgg cagtaacgat ctcgacgcga ccgttgttta cggcgtcaat caggatcaac
    14581 ttcgtgcgga gcaccgcatc gtttctaacg cttcctgtac cacgaattgc ataattcccg
    14641 tcatcaaatt gttagatgat gcgtacggta ttgagtccgg cactgtgacc acaattcact
    14701 ccgccatgca cgatcaacag gttattgatg cataccatcc tgacctgcgt cgcacccggg
    14761 cagccagcca gtcgatcatt ccggtcgata ctaaactggc cgccggtatc acacgatttt
    14821 ttccgcaatt taacgatcgc tttgaagcga ttgcggtacg tgtgccaacc ataaatgtga
    14881 cggcaatcga tttaagcgtg acggtgaaga aacctgtaaa agccaatgaa gtcaacctgt
    14941 tgctgcaaaa agcagcacaa ggtgcatttc atggtatagt tgactatacg gaattgccgt
    15001 tggtctctgt agattttaac cacgatccgc acagtgccat tgtcgatggc acccaaaccc
    15061 gggtcagtgg cgcacacctg atcaaaacgt tggtctggtg cgataacgaa tggggctttg
    15121 ctaaccgaat gctcgacacg acgttagcta tggctactgt tgctttcagg taagacgcaa
    15181 gcagcgtctg caaaactttt agaatcaacg agaggattca ccatgtctgt aattaagatg
    15241 accgatctgg atcttgctgg gaaacgtgta tttatccgtg cggatctgaa cgtaccagta
    15301 aaagacggga aagtaaccag cgacgcgcgt atccgtgctt ctctgccgac catcgaactg
    15361 gccctgaaac aaggcgcaaa agtgatggta acttcccacc tgggtcgtcc taccgaaggc
    15421 gagtacaacg aagaattctc tctgctgccg gttgttaact acctgaaaga caaactgtct
    15481 aacccggttc gtctggttaa agattacctc gacggcgttg acgttgctga aggtgaactg
    15541 gttgttctgg aaaacgttcg cttcaacaaa ggcgagaaga aagacgacga aatcctgtcc
    15601 aaaaaatacg ctgcactgtg tgacgtgttc gtaatggacg cattcggtac tgctcaccgc
    15661 gcgcaggctt ctactcacgg tatcggtaaa ttcgctgacg ttgcgtgcgc aggcccgctg
    15721 ctggcagctg aactggacgc gctgggtaag gcactgaaag aacctgctcg cccgatggtg
    15781 gctatcgttg gtggttctaa agtatctacc aaactgaccg ttctggactc cctgtctaaa
    15841 atcgctgacc agctgattgt tggtggtggt atcgctaaca cctttatcgc ggcacaaggc
    15901 cacgatgtgg gtaaatccct gtacgaagct gacctggttg acgaagcaaa acgtctgctg
    15961 accacctgca acatcccggt tccgtctgat gttcgcgtag caaccgagtt ctctgaaacc
    16021 gctccggcta ccctgaaatc tgttaacgac gtgaaagctg acgagcagat cctggatatc
    16081 ggtgatgctt ccgctcagga actggctgaa atcctgaaga atgcgaaaac cattctgtgg
    16141 aacggtccgg ttggcgtgtt cgaattcccg aacttccgca aaggtactga aatcgtggct
    16201 aacgctatcg cagacagcga agcgttctcc atcgctggcg gcggcgacac tctggcagca
    16261 atcgacctgt tcggcattgc tgacaaaatc tcctacatct ccactggcgg cggcgcattc
    16321 ctcgaattcg tggaaggtaa agtactgcct gcagtagcga tgctcgaaga gcgcgctaag
    16381 aagtaaaaaa tcacagggca gggaaacctg cccttgtttc agcgcgcttt tagagcacgc
    16441 attttttcat actctaaata attcgagttg caggaaggcg acaagagagt gaatccccag
    16501 gagcttacat aagtaagtga ctggggtgag cgaacgcaga cgcagcacat gcaacttgaa
    16561 gtatgacgag tataaggccc gacgatacag gacaagagac atgtctaaga tttttgattt
    16621 cgtaaaacct ggcgtaatca ctggtgatga cgtacagaaa gttttccagg tagcaaaaga
    16681 aaacaacttc gcactgccag cagtaaactg cgtcggtact gactccatca acgccgtact
    16741 ggaaactgct gctaaagtta aagcgccggt tatcgttcag ttctccaacg gtggtgcttc
    16801 ctttatcgct ggtaaaggcg tgaaatctga cgttccgcag ggtgctgcta tcctgggcgc
    16861 gatctctggt gcgcatcacg ttcaccagat ggctgaacat tatggtgttc cggttatcct
    16921 gcacactgac cactgcgcga agaaactgct gccgtggatc gacggtctgc tggacgcggg
    16981 tgaaaaacac ttcgcagcta ccggtaagcc gctgttctct tctcacatga tcgacctgtc
    17041 tgaagaatct ctgcaagaga acatcgaaat ctgctctaaa tacctggagc gcatgtccaa
    17101 aatcggcatg actctggaaa tcgaactggg ttgcaccggt ggtgaagaag acggcgtgga
    17161 caacagccac atggacgctt ctgcactgta cacccagccg gaagacgttg attacgcata
    17221 caccgaactg agcaaaatca gcccgcgttt caccatcgca gcgtccttcg gtaacgtaca
    17281 cggtgtttac aagccgggta acgtggttct gactccgacc atcctgcgtg attctcagga
    17341 atatgtttct aagaaacaca acctgccgca caacagcctg aacttcgtat tccacggtgg
    17401 ttccggttct actgctcagg aaatcaaaga ctccgtaagc tacggcgtag tgaaaatgaa
    17461 catcgatacc gatacccaat gggcaacctg ggaaggcgtt ctgaactact acaaagcgaa
    17521 cgaagcttat ctgcagggtc agctgggtaa cccgaaaggc gaagatcagc cgaacaagaa
    17581 atactacgat ccgcgcgtat ggctgcgtgc cggtcagact tcgatgatcg ctcgtctgga
    17641 aaaagcattc caggaactga acgcgatcga cgttctgtaa gtcattcctt tctgcttatc
    17701 tcaaggcccg ctctgcgggt ctttttttcg ccaaaagcaa aattgcctga tgcgctatgc
    17761 ttatcaggcc tacacgtatc ctgcaatata ttgaatttgc aggtttttgt aggccggata
    17821 aggcgttcac gccgcatccg gcatgacaaa gcgcacattg atatctgtct gagccccgct
    17881 ctgcggggat ttttttgcca aaagcaaggt gattcagatg agaaatctgt gatctatttg
    17941 gcaaaattat gctttattgt ttacccttgt cagactgccc gtcataaggc ggcggagtgt
    18001 atttctccat tttgagtcag ttgaaaagga atattgaatg gaagatttga atgttgtcga
    18061 tagcataaac ggcgcgggaa gctggctggt agctaaccag gcgctgctgc taagttatgc
    18121 agtaaacatc gtggcggcac tcgcgatcat catcgttggt ttgattatcg cgcggatgat
    18181 ttccaacgcg gtgaatcgcc tgatgatctc ccgtaaaatc gatgccactg ttgctgattt
    18241 tctttctgca ttagtccgtt acggtattat cgcctttacg ctaatcgcag ccctgggacg
    18301 cgtgggtgta caaacagcgt cagtcattgc tgtactcggt gccgcaggct tagctgttgg
    18361 tctggctttg caggggtcac tttctaacct ggccgctggc gtgttacttg tcatgttccg
    18421 cccgttccgt gccggagaat atgttgacct cggcggcgta gccggtactg tgctgagtgt
    18481 gcagattttc tccaccacca tgcgtactgc agacggtaaa attatcgtta ttccgaacgg
    18541 taaaattatt gccggaaata ttattaactt ctcccgcgag ccagttcgcc gtaacgaatt
    18601 tattattggc gtggcgtatg attccgatat cgatcaggtt aagcagatcc tgaccaatat
    18661 tatccagtct gaagatcgca ttttgaaaga tcgcgaaatg actgtgcgcc tgaacgaact
    18721 tggtgcatcg tcgattaatt tcgtggtccg cgtctggagc aacagcggcg atctgcaaaa
    18781 cgtgtactgg gatgtgctgg agcgtattaa acgtgaattt gatgccgccg gtatcagctt
    18841 cccgtacccg caaatggatg tgaactttaa gcgggtgaaa gaagacaaag ctgcgtaatc
    18901 aacgctgagg ccagataata ctctctggcc tctctcttat tagttattct gattgccaat
    18961 taatattatc aatttccgct aataacaatc ccgcgatata gtctctgcat cagatactta
    19021 attcggaata tccaacgtgt tttcttatta ctttcaaggt cttgctcttg gggcagctat
    19081 gattctgccg ctcggcccac aaaatgcttt tgtgatgaat cagggcattc gtcgtcagta
    19141 ccacattatg attgccttac tttgcgctat cagcgatttg gtcctgattt gcgcggggat
    19201 ttttggtggt agcgcgttat tgatgcagtc gccgtggttg ctggcgctgg tcacctgggg
    19261 cggcgtagtc ttcttgctgt ggtatggttt tggcgctttt aaaacagcga tgagtagtaa
    19321 tattgagtta gctagtgccg aagtcctgaa gcaaggcaga tggaaaatta tcgccaccat
    19381 gttggcagtg acctggctga atccgcatgt ttacctagat acttttgttg tactgggcag
    19441 ccttggcggg cagcttgacg tcgaaccaaa acgttggttt gcgctcggga caattagcgc
    19501 ctctttcctg tggttctttg gtctggctat tctcgcagcc tggctggcac cgcgcctgcg
    19561 cacggcaaaa tcacagcgca ttatcaatct ggtagtggga tgtgtcatgt ggtttatagc
    19621 cttgcagctg gcgagagacg gtattgctca tgcacaagcc ttgttcagtt aggcgctatc
    19681 tgatggaaaa ataaaacaga ggcgctaagc ttgcctccag agatcctgaa ttttgggcaa
    19741 gtcaattaac acggagagac taacgtgaag ttcaaagtta tcgccctggc ggcattaatg
    19801 ggtattagcg ggatggcagc gcaggctaac gaattgccgg atggaccgca tattgtcacc
    19861 tccggtacgg caagtgtgga tgcggtgcca gacattgcca ctcttgcgat tgaagttaac
    19921 gtggccgcga aggatgccgc tacagccaag aaacaggcag atgagcgcgt cgcacaatac
    19981 atttccttcc ttgaactcaa tcagatcgcg aaaaaagata tcagctcagc gaatttacgc
    20041 acccagccag attatgatta tcaggatggt aaaagtatcc tgaaaggcta ccgcgctgtg
    20101 agaacggtgg aagtcacgct ccgtcagtta gacaaactga attccttgct ggatggcgcg
    20161 ctgaaggcgg gtcttaacga aattcgttca gtgtcgctgg gcgtggcgca gccggatgcc
    20221 tataaagaca aagcgcgtaa ggcagcgatt gataacgcga ttcatcaggc gcaggaactg
    20281 gcgaacggct ttcatcgtaa actggggccg gtatatagcg tgcgctacca tgtttccaac
    20341 tatcagccca gcccaatggt gcggatgatg aaagccgatg ccgcgccggt gtctgcccag
    20401 gaaacttacg agcaggccgc tattcagttt gatgatcagg tcgatgtggt cttccagtta
    20461 gaacctgtgg atcaacaacc cgctaaaaca cctgcagcac aataatactt gtagcccgac
    20521 gtattcgtcg ggctaatgcc tttacccgat attgcctcct ttttctgtaa ttttctgtaa
    20581 aaccatgacg taatcaccta tctttgctcg cggtttcatc aaggataggc tgactgaaac
    20641 ggttaagcgg acaaggatgc ggggaggggg gatggacatt tttatttcga aaaagatgcg
    20701 caatttcatt ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat
    20761 gacggcttcg ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct
    20821 atttacccgc aaagataata atatcagcct caataaagcc ggtcaggaac tgtatcaaaa
    20881 actgttcccg gtttatcaac gactttctgc gattgataat gaaatccata actcagggcg
    20941 tcgttcacga gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca
    21001 gttgattagt ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga
    21061 aaacggcgtt atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca
    21121 tgtatctgca cgagttcagg aactggaaaa cctgactatc agtgaattac caccgctgcg
    21181 attgaacttt ctcgtttctc gccgttatga agagcgtcaa gagcaagagc tgcttcagga
    21241 gctgccctag ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc
    21301 gaatatgcga ccttgtggga tcaatcccac cattatttat cgtccctaca gttttatggc
    21361 caaaatcagc gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggcg
    21421 actggtgaat ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca
    21481 ggaatatctt tattcaatca gaaatcatcg ttataccgcc acgatgcttc agcatattgc
    21541 tgaagatcgt gacgggacaa accattaacc cagcatcgag ccggttgcaa ttaaattacg
    21601 gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt ccgccgggcg cattttccag
    21661 atagcgatgc agataatccc gatacatagg atgtgcacaa ttatcaatga tagtgcgggc
    21721 gcgttgaagc ggggaaagac cgcgcagatc ggcgatccct tgttcggtga tgatcacttt
    21781 gacgctgtgt tcgttgtgat caacatggct gcacattggc acgacggttg agatcttgcc
    21841 ttctttagca atcgacggag ccataaagat cgacagatac gcgttgcgtt caaaatcacc
    21901 gctgccgccg atgccgttca tcagatcgac cccggctacg tgcgttgagt tggcatgccc
    21961 gtaaatatca aactccaggc cgacgttcag agcgatgacg cccagacgac ggatgatttc
    22021 cgggttattg gaaatctcct gcggacgcaa cacaatgcgg ctggcaaagt aatccatatt
    22081 gtcgtaaatc ttgcgcaggg aatcggccga gattgtcagg ctggaggcgc tggccccgct
    22141 gattttgccg gtttccagta aatgcaccac cgattcctgt agcacttccg aatacatcat
    22201 aaacggagga atttccgggt tttcccccag acgcgccatt accgcattat tgatattgcc
    22261 cacgccactt tgcagcggca gaaattccgg cggaatacgc ccatgcgcca tttcctgcaa
    22321 taagaacgtg accacgttat cggcaatctg ctggcacatg ggattttgct tatccagcat
    22381 attaccggcg tcgggcaagt tggtttccac gacggcgaca atctttttcg gatcgatttg
    22441 cacatagcgg gtaccgacgc gatccattgc atggaagatc gacacgctat tgcgccgcgg
    22501 tggcgcgcca ggaatcacaa tatccgccag ttctgcaacg cgcggatcgt gatagtgatt
    22561 gagttcaatg atcactttct tcgctcgcag cagccaggtc ggcgcattac cgatcccgct
    22621 ggttaaccag actcgaccat ctggtgccag tgccgatgct tcaatgacgg caacatcaat
    22681 gtcgccgaag aaaccgtaat tgaccatttg cgccacttcg ctcaaatgca ggtcaacgaa
    22741 actcaccgcg ccctgattga tctttttacg taaaccggac gatgtttgat atggcgcacg
    22801 ccaggaaaca gcatcggcgt cagaaagtac atcgtcagcg gcggcgctga ttgacgcccc
    22861 cgtcagcagg cgaatttgat acggcttttt ggcctcatgc tgttcgttag ctctgcgggc
    22921 aatcgcggtg ggtagggctt tcggcgaacc cgctggggta aagccgctaa atgccaccat
    22981 gtcgttatgc tggataattt ctgccgcttc atcggcggtc atccttgtcc actgagtttc
    23041 catttttact ccttcgttcg cagggattaa tgaccaacga aattaggttt acgtttttcg
    23101 aggaaagcgt tcatcccttc ctggtaatct tcgctgtcat aaaccgcgcg gcgcatcccc
    23161 tgaatacgtt caaattcatc ggagttcatg gtgtgtgctt cgcccagtac acgcagctct
    23221 tctttgataa cggcaatggc taacggcgct ttctcagaga tgtggtgcgc catttgtaag
    23281 gtgaaatctt ccagttcttc cacttccaca acatggttga ggatgccgac agccagcgcg
    23341 cgctgggcgg tgatgggcga agcggtaaaa atcagctctt tgacaatgtg gaagcccgcg
    23401 tcgcgggtca ggttgtgaat gccgaccagg ttatacggga cgccgaggtt tacaggcgtc
    23461 attgagaagg ttgaggtact ggcggcgata atcagatcgg aactcatgat catttcaaat
    23521 gcgccacccc aaacactacc ttccaccatc gaaataatcg gtttcgggaa tttttggatc
    23581 atgcgggtga tttgacgcaa tggatcatca taggagagcg gatcgcgacc gccagacggc
    23641 agttcgtgaa tatcgtgacc tgcggagaag actttggatc cactcggcgc gcgcaaaatg
    23701 atacagcgaa tttccggccg gttgagatcg cttaacgcct gcataagatc atcaataaag
    23761 actttactta aggcattaag ttttcggcca tagttaaact caatgaccgc cactttgttg
    23821 atagtgacaa cgttaacata ctgataagac ataaaaattc ctttaatcaa aatattgcgt
    23881 ctggataaat tcactgagct gccgcaggcc ggtgcgcggt gagagcgtat tgtttttgac
    23941 cgctaaaagc gtctggcggt aatagcgatc gaaatcttca ttcgcgaaca ggtgattcag
    24001 tacttcttct tcggtctgct tacgcagcca ttccaccgat tgttgttgcc gcacttgttg
    24061 taaacgacca ctggcagtta gcgcggtttt gaagtcgatg atggcgtgcc agatctcatc
    24121 gattccacgt ttttccagtg cgctacaagt cagaacccgt ggctgccatt cgtcgtattt
    24181 acgtcgcaga atatgcaggg cactctcgta catatgccgg gcaatggcga cattggtatg
    24241 gttatcgcca tcgtctttgt tgataacgat cagatcagcc acttccatca gccctttttt
    24301 aatgccctgc agatcatcgc cgccaccggc aatttgcaac gagataaaac agtccaccat
    24361 gcgggcgact tctgtttccg actgcccgac gccaaccgtt tcgacaatca ctacgtcata
    24421 acccgctgct tcgcataaca gcattaattc ccgcgctcgc tgactggcac cgcccagatg
    24481 accggaggat ggtaccgggc gaataaacgc cgcttcggca cgcgccaggt cattcatgcg
    24541 ggttttatcc ccgagaatgc taccgccagt gaccgggctg ctgggatcga ccgcaataac
    24601 cgcgaccttt aatccctctc gaatcaacaa catgccaaag gcctcaagaa aggtactttt
    24661 ccccgcgccg ggggtgccgg taacgcccag tcgcagggtg ttaccgcagt acggcataat
    24721 ggcatcaagc agctgcgtac ttagtgcctg atgacgcggg tgacggcttt ccaccagcgt
    24781 catggcctgg gcgagtgtgg catgctcacc ctgacgtaag cggcgaatac tttctgccag
    24841 cgtggcttca ttaatcatga tgctggctta tcagattcag tacgtcgcgc acactgtcga
    24901 gcataggtgt acctggacca taaatcgccg ccacgccgcg ctcttgcagg aaggcgtaat
    24961 cctgcggtgg aatgacgcca ccggcgacta cgcagatatc ttcgcgtccc cattttttca
    25021 gcgcttcgac cagttccggg atcagcgttt tatgaccggc agccagtgag gatgcgccca
    25081 ctacgtgaac gtcattttct actgccaggc gggcgatctc ttcaggtgta gagaacatcg
    25141 ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct ggcgatcact ttcgcgccgc
    25201 gatcgtgtcc atccaggccc attttggcga tcagaatgcg cgggcgacga ccattgtcgg
    25261 caaggaactg ctccgtttgc gcaacaatgg catcgaactc ggaggccgat ttctcagatt
    25321 gatgatagct ttgcgcaatc acgccggtaa cacactggct tggcaccaga taacggtcga
    25381 aagcggcttc cagcgcatcg gaaatttcac ccagggtggc gcgaacgcga gcggcattaa
    25441 cagcggcagc cagcaggttt tcgttatgct gtgcggcgtg agtcagggcg ttcaacgcgg
    25501 cggttacggc ggcatcatca cgggtggcgc gaatgcgttc cagcgaagca atttgctcgt
    25561 tacgcaccat cacgttgtcg atctcaagta catcggtttc gtcttcgtga tccagtttgt
    25621 acttgttgac accaacgatg acacgcttgc cctggtcgat cagcgactgt tcgcgcgctg
    25681 aggcctcttc gatcattcgt tttggcagac ctgcttcgat cgctttcgcc atgccaccgg
    25741 cttcgtcgat ctgttggata atagctctgg cttgtttgac gatttgatcg gtcagcgact
    25801 caatgtaata ggatccggcc agtggatcga cggtgcggca gagttctgat tcttcctgga
    25861 tgatgatctg ggtgttgcgg gcaatgcgtg ctgagaaatc ggtaggcaaa ccaagcgctt
    25921 cgtcaaaggc gttggtatgc agtgactgag taccgcccag cgtcgcagcc agcgcttcaa
    25981 tggtggtgcg gataacgttg ttatacggat cctgttcagt caggctccag cctgaggtct
    26041 ggcagtgggt acgcagcgcc agtgatttcg ggtcctgtgc gccaaatcca ctgaccgctt
    26101 cgctccataa ataacgtgcc gcacgcaaca tggcgacgtt cataaacaga tccatgccga
    26161 tgccgaagaa gaacgacagg cgaggagcga agtcatcaat tttcagtccg gcagagattg
    26221 ctgctttgat gtactcaatc ccatcagcga gcgtaaatgc tacctgctgc acgcagttgg
    26281 cacccgcttc acccatgtgg taaccgctga tactgatggt attaaatcgc ggcatgttgc
    26341 cggaacacca ggcgatgatg tcggcgataa tgcgcattga cggttttggc gggtaaatat
    26401 aggtgttgcg gcagaggtac tctttgagaa tatcgttttg aatggtgccg gtcagtttat
    26461 caggtgtaac accttgctct tccgcggcga tgatataaaa cgccagtact ggtagcactg
    26521 cgccattcat ggtcatcgaa accgacattt tatccagcgg gatctggtcg aacaggactt
    26581 tcatatcttc cacggtgtcg atagcgacgc ccgttttgcc gacgtcaccc gccacgcgtg
    26641 ggttatcgga gtcgtagcca cggtgggtgg caaggtcaaa cgcaacggaa agaccttttt
    26701 gcccggcggc caggttacgg cgataaaaag cgttggactc ttttgctgtt gaaaaaccag
    26761 catactgacg gatggtccac ggttgggcgg tatacatagt ggcacgcggg ccacgaacgt
    26821 agggcggcaa accaggaagg gtacctgtca cctccagatt atcgagatcg gcttcggtat
    26881 acagcggctt gatggcgatc ccttccgcgg tttgctgaac cagcgagtcg acagttttct
    26941 cccgacggct caattccttg ttggcaagct gttgccactc ctgctcgtta gacattattt
    27001 gctccgtaaa acgcaatcaa aatgagggta tttgcgctta cggtgagctt tttacgtggg
    27061 tttgtcgcca gcaaaaaaaa tgcattcaga cgtcgttttt tgccgcctga gatttgtcta
    27121 ttttgtgatg tgattcaagg aatacaaaaa gaagtgagga taacgcctga tatgtgctat
    27181 caggcattat ttgatggatt aatcctgacg aaggactttg tgaccataat cgagcaacgc
    27241 atcagtgact ttacgcatca tgcggctttc aggagcaaag cggtgccagt agagcatccg
    27301 gcgttgaaat agccctggcg ttaagtcaat cagttcaccg ctggctagtt ctttctcgat
    27361 ttgcaggtgc gggatcatac agcaggtggt gccctggcga gcaagttgca cgaacgcttc
    27421 tgaagaatta acgatatggc agggcacgct gcctggaggc agatcgaagt tttgctgcaa
    27481 aaaggcctgg tgcatatcgt caagatggtc aaacgcgacc actggcgctt tcagtaatgc
    27541 cgaacgcgtt acgccgttag ggaaatattt ttcggcaaag ggttttgagc tgacgaacag
    27601 atagtcgagc gcaccaagtt tatcgacaag acaactcggc agcgcctgat gttgaatact
    27661 caccgcgccg accacttcgc cgcgacgcag acgttcctga gtgcgggttt catcttctac
    27721 ctgcaagttg aggcggatag gcgaatcagc caatacagga gccagtgccg gaagcaacca
    27781 cgtcgccaga ctgtcggcat tgaccgccag tgaaagcagc agcggagtcg aaccggtttg
    27841 ttcatcgccc agccactctt cttccagcaa ctccacctgg cgcagcagtg ccagcagttt
    27901 ttgcccttgt tccgtcgggc gcggcggtac ggtacgcacc aacagcggct gcccgaacat
    27961 attttccagt tgcttaatgc gctgtgagac ggctgattgt gtaatgcaca gcttttgtgc
    28021 cgcgcgctca aatcctcgtt cacgtatcac cgcatccagt gcctgtaatg ttctgtagtc
    28081 cgggcgtttc attgctgttg tctgctcctg gtaaatgctt tttcctgcac tatgacacaa
    28141 ttttgtgtca ggttgcatat accgcagccg tcggcgggta acatttgtat caatggttaa
    28201 acgcgcagct taaaagatca gggatttgcg ctattgcatt tcgcttagca acttcgcgcc
    28261 ctggttatgt tgaatggcgt ggcgttattg cctctgttcg caccgtggag cggcataccc
    28321 agaccgttgt atgctttatt tgcggggcga aaggggatgc ccgccattgc gcgatatttt
    28381 atggatgagt taaccacgcg gcttgccaac ggggtctgaa tcgctttttt tgtatataat
    28441 gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg caggatgaat tgaaaaaagc
    28501 agtaggatgg gcggcacttc agtatgttca gcccggcacc attgttggtg taggtacagg
    28561 ttccaccgcc gcacacttta ttgacgcgct cggtacaatg aaaggccaga ttgaaggggc
    28621 cgtttccagt tcagatgctt ccactgaaaa actgaaaagc ctcggcattc acgtttttga
    28681 tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc gcagatgaaa tcaacggcca
    28741 catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt gaaaaaatca ttgcttcggt
    28801 tgcagaaaaa tttatctgta ttgcagacgc ttccaagcag gttgatattc tgggtaaatt
    28861 cccgctgcca gtagaagtta tcccgatggc acgtagtgca gtggcgcgcc agctggtgaa
    28921 actgggcggt cgtccggaat accgtcaggg cgtagtgacc gataacggca acgtgatcct
    28981 cgacgtccac ggcatggaaa tccttgaccc gatagcgatg gaaaacgcca taaatgcgat
    29041 tcctggcgtg gtgactgttg gcttgtttgc taaccgtggc gcggacgttg cgctgattgg
    29101 cacacctgac ggtgtcaaaa ccattgtgaa atgatctgac gggggaacct cccccgttaa
    29161 aaaaattctc ttcattaaat ttggtgacat gtgtcacgtt tttacctgga tattgtcgat
    29221 tgctctaaat aaatcctcta aaccagcata ttcaaccaag aaataccttt gcgtgatatt
    29281 tcctcaacat cgcgacgcaa acgttcatat tgccgcaata ttattttttg atatgttgaa
    29341 aggcggatgc aaatccgcac acaacatttc aaagacagga ttgggtaaat ggcaaaggta
    29401 tcgctggaga aagacaagat taagtttctg ctggtagaag gcgtgcacca aaaggcgctg
    29461 gaaagccttc gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc gctggatgat
    29521 gaacaattaa aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat
    29581 ctgactgaag acgtgatcaa cgccgcagaa aagctggtcg ctattggctg tttctgtata
    29641 ggaacaaacc aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt atttaacgca
    29701 ccgttctcaa atacgcgctc tgttgcggag ctggtgattg gcgaactgct gctgctattg
    29761 cgcggcgtgc cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa actggcggcg
    29821 ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca tattggtacg
    29881 caattgggca ttctggctga atcgctggga atgtatgttt acttttatga tattgaaaac
    29941 aaactgccgc tgggcaacgc cactcaggta cagcatcttt ctgacctgct gaatatgagc
    30001 gatgtggtga gtctgcatgt accagagaat ccgtccacca aaaatatgat gggcgcgaaa
    30061 gagatttcgc taatgaagcc cggctcgctg ctgattaatg cctcgcgcgg tactgtggtg
    30121 gatattccgg cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc ggcaatcgac
    30181 gtattcccga cggaaccggc gaccaatagc gatccattta cctctccact gtgtgaattc
    30241 gacaacgtcc ttctgacgcc acacattggc ggttcgactc aggaagcgca ggagaatatc
    30301 ggcctggaag ttgcgggtaa attgatcaag tattctgaca atggctcaac gctctctgcg
    30361 gtgaacttcc cggaagtctc gctgccactg catggtggtc gtcgtctgat gcacatccac
    30421 gaaaaccgtc cgggcgtgct aactgcgctg aacaaaatct tcgccgagca gggcgtcaac
    30481 atcgccgcgc aatatctaca aacttccgcc cagatgggtt atgtagttat tgatattgaa
    30541 gctgacgaag acgttgccga aaaagcgctg caggcaatga aagctattcc gggtaccatt
    30601 cgcgcccgtc tgctgtacta attccccttc tctgaaaatt aacgggcagg tcactgactt
    30661 gcccgttttt ttatcccttc tccacaccgt tgccgcgttg cgcattctac tttgcgagtc
    30721 tgctccagaa aataaatcaa agtcagatca atgcgttgtg tttatttaat ggggatttac
    30781 gatggcggga cagcatgggg ctgtaacggt gaagccctca ccgaagcgag ggcttaaagg
    30841 agaagggtta tgatgcgact tgtcatcata ctgattgtac tgttactgat aagtttcgga
    30901 gcttattaac agtcagtctc aggggaggag tcatcctccc ttacccttac tcactaaatt
    30961 aggtcaaaga atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc tgaaccgaac
    31021 tttttgatag ccacttcatc gcccacgttt actgccgtaa ttaatccctt attcctgcaa
    31081 tgtatgtctg ccttacatga cgttttacga gtgcgctcga agaaaagatt taaaggtaga
    31141 tcaatgcgtt gtggttattt aaagagagtt tacgatggca gggcagcatg gggctgtaac
    31201 ggtaaagccc tcaccgaagc gagggcttga aggagaaggg ttatgatgcg acttgtcatc
    31261 atactgattg tactgttact gataagtttg ccagcttatt aacagtcagt ctcaggggag
    31321 gagtcatcct cccttaccct tactcactaa attaggttaa agaatcaacg atgtcaatca
    31381 gggcgatgca ggtgtatcgc ccttaccact cccagacttt cgacggtgta accaccgcag
    31441 gaagagggat atcccactct tcaacgggga gtttttccac caactgacaa tcatgcgcat
    31501 aacccaccgg ttgcgtttta tagtgctgcc agttttgtaa ggtccgatca taaaaaccac
    31561 cgcccattcc caggcgctga ccgtactcat caaaggcgac cagcggtgtg atcagcacgt
    31621 ctaatcggga aagggggagc acgtcacgca catccagttt tggctcatgg attttcaacc
    31681 tgttcatcac cagttcgctt tgcggatggt aattcaggaa cagcaaatta ccggcactaa
    31741 agggatgcaa aactggaaga tatacgcgct taccggcgcg ccagagttgt tctataagcg
    31801 gctgggtgtc gagttcacca tcaaaagaga ggaatacagc gaccgtatgc gccatcacca
    31861 ccgggggata agtcatcatc cgggtagcgg cttgttgacc catttcctgc tgttgttccg
    31921 gcgttaacgc acgacgacgt tgccgaatca ttttgcggat ttcttgtcgg gataatgtca
    31981 gtgggagttc tggtagttgc gtcatggctg gtgccagata agaagggaat ctccgagatg
    32041 ccgccgcagg ctgtaaccct tgaacccttg gttcaaggtg aatgtgtcgt cgcagtttta
    32101 aggcttctcg gacggaccga gcatgctcac caaccgcgga gcgccacatt cttgtggtat
    32161 gaaatatcgg ctcaggggac tggcccgctt gcgaacatct cagagaaatt ttgtcttcac
    32221 ggttactcta ccacagtaaa ccgaaaagtg tcattcaaag ttttggttag ttttttcggt
    32281 gatgcgacct tgttcaagta acgcttgttc tatggtctgc tgcagcatcc gaatacgctg
    32341 ttccatactt gccgcgtagt cacgagtctt cgctttttct tgcgctaact catagctgat
    32401 attcaatgcg gcaatgaaga ccaactgttc agtatttgtg actctagtgc gttctttcag
    32461 atcttgcaac cgttggttca gatcgtccgc tgcctgattc aacgcatccc tttggtcagg
    32521 cgggcagttc acacgcagtg aacggccaaa aatttggata tcgacgggtt gtgcagacat
    32581 gccaccttcc tgctgattga ctgcgctgcc ttcgtcttta gacccatggt ctgcgaaggg
    32641 gcgacactat agctaccctg atgagaagag acaagccctt ttctggtcca ccagggccca
    32701 aagtggtagc atatcatgaa tattcctccc tttgacgacg aatgcttatg tctatacaga
    32761 acgaaatgcc tggttacaac gaaatgaacc agtatctgaa ccaacaaggg acgggtctga
    32821 cccctgctga gatgcatggt ttaatcagtg ggatgatatg tggcggtaac gatgacagct
    32881 catggctacc gctacttcac gacctgacga acgaaggcat ggctttcggt catgagctgg
    32941 cacaggcatt gcgtaaaatg cactctgcca ccagcgatgc cctgcaggat gacggcttcc
    33001 tttttcagct ttatctgcct gatggcgatg atgtcagcgt tttcgatcgg gctgatgcat
    33061 tggcaggttg ggtcaatcac ttcctgcttg gtcttggtgt tacgcaaccg aagctggata
    33121 aagtgaccgg cgaaaccggt gaagctatcg acgatctgcg taacattgcg caactgggtt
    33181 acgacgaaga cgaagatcag gaagagcttg aaatgtcgct tgaagagatc atcgaatacg
    33241 ttcgtgttgc cgcgctgtta tgccacgaca cctttactca tccgcaaccg accgcgccag
    33301 aagtacaaaa accgactcta cactaaaaac aaaaaacgta aggagagtgt tatgagtgag
    33361 atatcccggc aagagtttca gcgtcgccgt caggccctgg tggagcaaat gcaacccggc
    33421 agcgccgcgc tgatttttgc tgcaccagaa gtaacacgta gcgccgacag cgaatacccc
    33481 tatcgtcaga acagtgactt ctggtacttc accggcttta acgaaccgga agcggtgttg
    33541 gtgctgatta aaagcgatga cactcataac cacagcgttc tgtttaaccg cgttcgcgac
    33601 ctgacggcgg agatctggtt tggccgtcgc ttaggccagg atgccgcgcc agagaaactg
    33661 ggcgttgacc gcgcactggc attcagcgaa attaatcagc aactttatca actacttaat
    33721 ggtctggatg tggtttacca tgctcagggc gaatatgcat atgctgatga aatcgtgaac
    33781 agtgcgctgg aaaaactgcg taaaggttcg cggcaaaatc tcaccgcacc ggcaacgatg
    33841 atcgactggc gtcctgttgt tcatgaaatg cgcctgttca aatcgccaga agagattgcc
    33901 gtactccgcc gcgcgggaga aatcaccgcc atggcacata cacgggcgat ggaaaaatgc
    33961 cgtccgggaa tgttcgagta ccatctggaa ggcgaaattc accacgaatt taaccgccac
    34021 ggtgcgcgct atccgtccta taacaccatt gtaggcagcg gtgaaaacgg ctgcatttta
    34081 cactacaccg aaaacgagtg tgaaatgcgc gacggcgacc tggtgttgat tgacgcaggc
    34141 tgcgaataca aaggttacgc aggcgatatc acccgtacct tcccggtcaa cggcaaattc
    34201 acccaggccc agcgcgaaat ctacgacatt gtgctggagt ctcttgaaac cagcctgcgc
    34261 ctgtatcgtc cgggaacctc cattctggaa gtcaccggtg aagtggtgcg cattatggtt
    34321 agcggcctgg taaaactcgg catcctgaaa ggtgatattg atgaactgat cgctcagaac
    34381 gcccatcgtc ctttctttat gcatggcctt agccactggt taggactgga tgtccatgac
    34441 gttggcgttt atggtcagga tcgctcgcgc attctggaac cggggatggt actgaccgta
    34501 gagcctggtc tgtatatcgc gccggatgcg gatgtgccag aacaatatcg cggtatcggc
    34561 attcgtattg aagacgacat tgtgattacc gaaaccggca acgaaaacct caccgccagc
    34621 gtggtgaaaa agccggaaga aatcgaagcg ttgatggctg ctgcgagaaa gcaatgagcg
    34681 taatcatcgt cggtggcggc atggcgggcg cgacgctggc gctggctatt tcccggttaa
    34741 gtcacggggc gctgccggta catttgattg aagcgactgc gccagagtca catgctcatc
    34801 cgggctttga tggacgagcg atagcgctgg cggcgggtac ctgtcagcaa ctggcgcgca
    34861 tcggcgtttg gcaatctctg gcgcattgcg caactgccat caccaccgtg catgtcagcg
    34921 atcgaggtca cgctggattt gtcaccctcg ccgcagaaga ttaccaactg gcggcgctgg
    34981 gacaggttgt cgaattgcac aatgtcgggc aacggctgtt tgcattgctg cgtaaagcac
    35041 ctggcgtaac gctgcattgc cctgatcgcg tggctaacgt tgcccgtact cagagtcacg
    35101 ttgaagtgac gctggagggt ggcgagacgc tgacgggccg cgtgctggta gccgctgatg
    35161 gcacccattc agcgttagcc actgtgtgcg gcgttgactg gcagcaggag ccttacgaac
    35221 aactggctgt gattgccaac gttgcgactt ccgttgcgca tgaagggcgc gcttttgaac
    35281 gctttacgca acatggcccg ctggcgatgt tgccgatgtc tgacggacgc tgttcgctgg
    35341 tctggtgtca tccactggaa cggcgcgaag aggtgctgtc gtggagcgac gagaagtttt
    35401 gccgtgaact ccagtcggcc tttggctggc gacttgggaa aattacccac gctggtaaac
    35461 gcagtgctta tccgctggcg ttaacccacg ccgccagatc tattacccat cgtaccgtgc
    35521 tggtgggcaa tgcggcgcaa accctgcacc cgattgccgg gcaagggttc aacctcggta
    35581 tgcgagatgt catgagtctt gcggaaaccc tgactcaggc gcaggagcgc ggagaagaca
    35641 tgggtgatta cggcgtattg tgtcgttatc agcagcgtcg acagagcgat cgcgaagcaa
    35701 ccattggcgt cacggacagc cttgtacatc tttttgccaa ccgttggacg ccactggttg
    35761 tcgggcgcaa catcgggctg atgacgatgg aattattcac cccggcacgc gatgtgctgg
    35821 cgcagcgcac cctcggttgg gtggcgcgtt gaaggcggtt attaaggaat aaacaatgca
    35881 aagtgttgat gtagccattg ttggcggcgg catggtgggg ctggcggttg cctgcggctt
    35941 acagggtagc ggcttacgcg ttgccgtact ggagcagcgc gtaccggaac ctctggcggc
    36001 ggatgcacca ccacaactgc gcgtttcggc tatcaatgcc gccagcgaaa aattactcac
    36061 ccgtcttggc gtctggcagg acattctctc tcgccgagcc agctgttatc acggtatgga
    36121 agtgtgggac aaagacagtt ttggtcacat ctcgtttgac gatcaaagca tgggctatag
    36181 ccatcttggg cacatcgttg aaaactcagt gattcactac gcgctgtgga acaaagcgca
    36241 gcagtcgtca gatatcactc ttttggcccc cgcagaatta cagcaggtcg cctggggaga
    36301 aaatgaaacc ttcctgacgc tgaaagatgg cagtatgtta acggcgcgtc tggtgattgg
    36361 cgcggacggc gctaattcct ggttgcgcaa caaagccgat attccgctga ctttctggga
    36421 ttatcagcat cacgcgctgg tagcgaccat acgcacggaa gaaccgcatg atgcggtggc
    36481 gcggcaggtt ttccatggcg aaggcattct ggccttttta ccgcttagcg atccgcatct
    36541 ttgctcgatt gtctggtcac tgtcgccaga ggaagcgcag cggatgcagc aggcaagtga
    36601 agacgaattt aatcgcgcgt taaatatcgc ttttgataat cgcctgggct tatgcaaggt
    36661 tgagagcgcg cgtcaggtgt tcccactgac ggggcgttat gcgcgccagt ttgccgcgca
    36721 ccgtctggcg ctggtgggcg acgccgcgca taccattcac ccgctggcgg ggcagggggt
    36781 aaatcttggc tttatggatg ctgcagagct gattgctgaa ctgaaacggt tgcatcgtca
    36841 gggtaaagac atcgggcagt acatttatct gcgtcgctat gagcgtagcc gcaagcacag
    36901 tgcggcgctg atgctggctg gtatgcaggg attccgcgat ctgttttctg gtaccaatcc
    36961 ggtgaaaaaa ctgctgcgtg atattggtct gaaactggcc gacacgcttc ctggcgttaa
    37021 accacaactt atccgccagg caatgggatt aaacgatttg cctgaatggc tgcgttaaaa
    37081 atttctcttc tgctgtttat ttgataccca tcacactttc atctcccggt tttttcgccg
    37141 ggagattttc ctcatttgaa ataaactaat ttcacctccg ttttcgcatt atattttcta
    37201 atgtcacgaa tttttgattt agtgtttttt gacatttttt cagctcttaa tattgcctta
    37261 ttcaaattaa ctttctcatc acatcatctt tatatagaaa cttgtgtatt ttttggtttt
    37321 ttattctgtt gcgattttgg cgttttttaa ccataagcta atgtgatgat caattttacc
    37381 ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa agagaacgat tgcgttgggg
    37441 accgggagtg gctccgatgc tgggtttcgt ggtgataatt tcaccatgaa aaagttgtca
    37501 gccccgctta ttcaatgagg acaagatggc acaacagact cctttgtacg aacaacacac
    37561 gctttgcggc gctcgcatgg tggatttcca cggctggatg atgccgctgc attacggttc
    37621 gcaaatcgac gaacatcatg aggtacgtac cgatgccgga atgtttgatg tgtcacatat
    37681 gaccatcgtc gatcttcgcg gcagccgcac ccgggagttt ctgcgttatc tgctggcgaa
    37741 cgatgtggcg aagctcacca aaagcggcaa agccctttac tcggggatgt tgaatgcctc
    37801 tggcggtgtg atagatgacc tcatcgtcta ctactttact gaagatttct tccgcctcgt
    37861 tgttaactcc gccacccgcg aaaaagacct ctcctggatt acccaacacg ctgaaccttt
    37921 cggcatcgaa attaccgttc gtgatgacct ttccatgatc gccgtacaag ggccgaatgc
    37981 gcaggcaaaa gctgccacac tgtttaatga tgctcagcgt caggcggtgg aagggatgaa
    38041 accgttcttt ggcgtgcagg cgggcgatct gtttattgcc accactggtt ataccggtga
    38101 agcgggctat gaaattgcgc tgcccaatga aaaagcggcc gatttctggc gtgcgctggt
    38161 ggaagggggc gttaagccat gcggtctggg cgcgcgtgac acgctgcgtc tggaagcggg
    38221 catgaatctt tatggtcagg agatggacga aaccatctct ccgttagccg ccaacatggg
    38281 ctggactatc gcctgggaac cggcagatcg tgacttcatc ggtcgtgaag ccctggaagt
    38341 gcagcgtgag catggcacag aaaaactggt tggtctggtg atgaccgaaa aaggcgtgct
    38401 gcgtaatgaa ctgccggtac gttttaccga tgcgcagggc aaccagcatg aaggcattat
    38461 caccagcggg actttctccc cgacgctggg ttacagcatt gcgctggcac gcgtgccgaa
    38521 aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt gaaatgccgg ttaaagtgac
    38581 aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga tttacttttt tggagattga
    38641 ttgatgagca acgtaccagc agaactgaaa tacagcaaag aacacgaatg gctgcgtaaa
    38701 gaagccgacg gcacttacac cgttggtatt accgaacatg ctcaggagct gttaggcgat
    38761 atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta gcgcgggcga tgactgcgcg
    38821 gttgccgaat cagtaaaagc ggcgtcagac atttatgcgc cagtaagcgg tgaaatcgtg
    38881 gcggtaaacg acgcactgag cgactccccg gaactggtga acagcgaacc gtatgcaggt
    38941 ggctggatct ttaaaatcaa agccagcgat gaaagcgaac tggaatcact gctggatgcg
    39001 accgcatacg aagcattgtt agaagacgag taacggcttt attcctcttc tgcgggagag
    39061 gatcagggtg aggaaattta cacctcaccc tcactctctt cgcaaggaga gaggttcaca
    39121 attcactgca cgtttcagga accatcgctc atgacacaga cgttaagcca gcttgaaaat
    39181 agcggcgctt ttattgaacg ccatatcgga ccggacgccg cgcaacagca agaaatgctg
    39241 aatgccgttg gcgcacaatc gttaaacgcg ctgaccggcc agattgtacc gaaagatatt
    39301 cagcttgcga ccccgccgca ggttggcgca ccggcgaccg aatacgccgc gctggcggaa
    39361 ctcaaggcta ttgccagtcg caataaacgc ttcacgtctt acatcggcat gggttacacc
    39421 gccgtgcagt taccgccggt tatcctgcgt aacatgctgg aaaatccggg ctggtatacc
    39481 gcgtatactc cgtatcaacc tgaagtctcg cagggccgcc ttgaggcact gctcaatttc
    39541 cagcaggtaa cgctggattt aaccgggctg gatatggcct ctgcttctct tcttgacgaa
    39601 gccaccgctg ccgccgaagc aatggcgatg gcgaaacgcg tcagcaaact gaaaaatgcc
    39661 aaccgcttct tcgtggcttc cgatgtgcat ccgcaaacgc tggatgtggt ccgtactcgt
    39721 gccgaaacct ttggttttga agtgattgtc gatgatgcgc aaaaagtgct cgaccatcag
    39781 gacatcttcg gcgtgctgtt acagcaggtt ggcactaccg gtgaaattca cgactacact
    39841 gcgcttatca gcgaactgaa atcacgcaaa attgtggtca gcgttgccgc tgatattatg
    39901 gcgctggtgc tgttaactgc gccgggtaaa cagggcgcgg atattgtttt tggttcggcg
    39961 caacgctttg gcgtaccgat gggttacggt ggcccacacg cggcattctt tgcggcgaaa
    40021 gacgaataca aacgctcaat gccaggccgt attatcggtg tatcgaaaga tgcagctggc
    40081 aataccgcgc tgcgcatggc gatgcagact cgcgagcaac atattcgtcg tgagaaagcg
    40141 aactccaaca tttgtacttc tcaggtgctg ctggcaaaca tcgccagcct gtatgccgtt
    40201 tatcacggcc cgattggcct gaaacgtatc gctaaccgca ttcaccgtct gaccgatatc
    40261 ctggcggcag gcctgcaaca aaaaggtctg aagctgcgcc atgcgcacta tttcgacact
    40321 ctgtgtgtgg aagtggccga caaggcgggc gtactggcgc gtgccgaagc ggctgaaatc
    40381 aacctgcgta gcgatatcct gaacgcggtt gggatcaccc ttgatgaaac caccacgcgc
    40441 gaaaacgtga tgcagctttt cagcgtacta ctgggggata accacgggct ggacatcgac
    40501 acgctggaca aagacgtggc tcacgacagc cgctctatcc agcctgcgat gctgcgcgac
    40561 gacgaaatcc tcacccatcc ggtgtttaat cgctaccaca gcgaaaccga aatgatgcgc
    40621 tatatgcact cgctggagcg taaagatctg gcgctgaatc aggcgatgat cccgctgggt
    40681 tcctgcacca tgaaactgaa cgccgccgcc gagatgatcc caatcacctg gccggaattt
    40741 gccgaactgc acccgttctg cccgccggag caggccgaag gttatcagca gatgatcgcg
    40801 cagctggctg actggctggt gaaactgacc ggttacgacg ccgtttgtat gcagccgaac
    40861 tctggcgcac agggcgaata cgcgggcctg ctggcgattc gtcattatca tgaaagccgc
    40921 aacgaagggc atcgcgatat ctgcctgatc ccggcttctg cgcacggcac taaccccgct
    40981 tctgcacata tggcaggaat gcaggtggtg gttgtggcgt gtgataaaaa cggaaatatc
    41041 gatctgactg atctgcgcgc gaaagccgaa caggcaggtg ataatctctc ctgcatcatg
    41101 gtgacctatc cttccaccca cggtgtgtat gaagaaacga tccgtgaagt gtgtgaagtc
    41161 gtgcatcagt tcggcggtca ggtttacctt gatggcgcga acatgaacgc ccaggttggc
    41221 atcacctcgc cgggctttat tggcgcagac gtttcacacc ttaacctgca taaaactttc
    41281 tgcattccgc acggcggtgg tggtccgggt atgggaccga tcggcgtgaa agcgcatctg
    41341 gctccgtttg taccgggtca tagcgtggtg caaatcgaag gcatgttaac ccgtcagggc
    41401 gcggtttctg cggcaccgtt cggtagcgcc tctatcctgc caatcagctg gatgtacatc
    41461 cgcatgatgg gcgcagaagg gctgaaaaaa gcaagccagg tggcaatcct caacgccaac
    41521 tatattgcca gccgcctgca ggatgccttc ccggtgctgt ataccggtcg tgacggtcgc
    41581 gtggcgcacg aatgtattct cgatattcgt ccgttgaaag aagaaaccgg catcagcgag
    41641 ctggatattg ccaagcgcct gatcgactac ggtttccacg cgccgacgat gtcgttcccg
    41701 gtggcaggta cgctgatggt tgaaccgact gaatctgaaa gcaaagtgga actggatcgc
    41761 tttatcgacg cgatgctggc tatccgcgca gaaattgacc aggtgaaagc cggtgtctgg
    41821 ccgctggaag ataacccgct ggtgaacgcg ccgcacattc agagtgaact ggtcgccgag
    41881 tgggcgcatc cgtacagccg tgaagttgcg gtattcccgg caggcgtggc agacaaatac
    41941 tggccgacag tgaaacgttt ggatgatgtt tacggcgacc gtaacctgtt ctgctcctgc
    42001 gtaccgatta gcgaatacca gtaattcact gattcgacta tcttctaaag gcgcttcggc
    42061 gcctttttag attgttaata aagtgcgctt tgtgtgtttc ggctgaggcg taatataaaa
    42121 tattgcgagc tcaatatatt aaaagagtgc caggcctgat gagcgtagcg catcaggcac
    42181 atcggctaat tacagattct cgccgttgct ggcaatcact tctttgtacc agttaaagct
    42241 cttcttacgt gaacgcgaca tatcgccagt accgtcgtca tgtttattca catagataaa
    42301 gccgtagcgt ttgctgtact gcccggtagt gaacgacacg cagtcgatgc agccccacgg
    42361 tgtgtagccc atcagatcca cgccatcgta agttactgct tttttcatct cttcaatatg
    42421 ggcgcgcagg tagtcaatgc ggtagtcgtc gttgatgctg ccatcttctt ccactttgtc
    42481 gtaagcgcca aaaccgtttt cgacaataaa cagcggcttc tggtaacgct catacagttc
    42541 gcaaagtgcg taacgcagac ccaccggatc aatctgccag ccccagtcag atgctttaac
    42601 atacgggttt ggtacgctgc cttcaaaacc agagatcgca tcgccggtgc cgccttcggc
    42661 cttcactgcg ttggtcatgt aatagctgaa accaagataa tcgcaggtgc cttcgcgcag
    42721 tacatcgaga tcgctatctt ccattttgat gttaaatccg cgacgctccc actcgttcaa
    42781 cacatacgac gggtaatagc cgcgtagctg cacatcggta aagacgtagc gttcgcgcat
    42841 cgactcctga gcgaacatca catcgtccgg gttacatgag tagggataga gcggcaccat
    42901 cgccagcata cagccgactt tcatctccag gttaatgcga cgcgcggctt tcactgccag
    42961 ggcgctggcg acaaactggt gatgcagcac ctgatacatc gtctcttccg ggttttcatg
    43021 ctcggtatac accacgccgg agcagcagta accgaacagc ggtgcacgcc agttacgctg
    43081 gttgttaatt tcgttgaagg tcatccagta tttgactttg tgcttatagc gttcaaatac
    43141 cacttccgcg aaacgtacaa agaaatcaac cactttacgg ttggtccagc taccgtattg
    43201 ctgcaccaga tgcagcggca tttcaaagtg ggagagggtg atcaccggtt cgatgttgta
    43261 tttcagcagt tcatcgaaca gagagtcgta gaacttcagc ccttcttcgt ttggctgagc
    43321 ttcatcgcct tttggaaaaa tgcgcgtcca ggcaatggat gtacgaaaac atttgaagcc
    43381 catttcggca aatagcttga tgtcttcctt atagtgacca taaaaatcaa cggcttcatg
    43441 gtttggatag tatttgcctg gcaagacttc tttggttatt tcgcgcggca cgccgtgtgc
    43501 gccaccggtc agaacgtcac aaatgctcgg cccttttccg cctttgttcc agccgccttc
    43561 gacctgatga gcggcaactg cgccgcccca taagaaatct ttcggtaagg tgagtttttt
    43621 cactatcata ggctccagat gagtcaattc ttacaggaaa tagtaccatg cagccaaacg
    43681 acatcacttt ttttcaacgt ttccaggatg acattctggc tgggcgtaaa accatcacca
    43741 tccgcgacga gtctgaatcg cacttcaaaa cgggtgatgt gcttcgtgtc ggacgttttg
    43801 aagatgacgg ttatttttgc acgattgaag tcaccgcaac ctcaaccgta acgctggata
    43861 cgctgacgga aaaacatgca gagcaggaaa atatgaccct gactgaactg aaaaaggtca
    43921 ttgccgacat ctatcccggc cagacacagt tttatgtgat tgaatttaaa tgcctttaaa
    43981 ttgaactcac gcctgttagt tgaaatttcg catttaattt catgattttt aggagattag
    44041 tctcaaatca gaatttattt tagctaacag gtgttcactg gaactattct cagttacgct
    44101 ggagaggtat acatgtgcgt gtacgtttct ccggagtaag ttatggttca gaagcccctc
    44161 attaagcagg gatattcgct ggcagaggaa attgccaaca gcgtcagtca cggcattggg
    44221 ttggtgtttg gtatcgttgg gctggtgttg ctactggttc aggcggtgga tctcaatgcc
    44281 agcgccacag cgataaccag ctacagcctc tatggcggca gtatgatcct gctgttcctc
    44341 gcttcgacgc tctatcacgc cattcctcat caacgggcaa aaatgtggct gaagaaattt
    44401 gaccattgcg ctatttacct gttgattgcc ggaacctaca cgccgttttt gctggtgggg
    44461 ctggattctc cgttagcgcg cgggttgatg attgttatct ggagcctggc attgctgggg
    44521 attctgttca aactgaccat cgcgcaccga ttcaaaattt tatctctggt gacttatctg
    44581 gcgatgggct ggctgtcgct ggtggtaatt tatgaaatgg cagttaagct cgcggcgggc
    44641 agcgttacct tactggcggt aggcggcgtg gtttattcgc tcggggtgat tttctacgtc
    44701 tgcaaacgca ttccatacaa ccatgccatc tggcacggct tcgtgctcgg cggtagtgtg
    44761 tgccactttc tggcgatcta tttgtatatt gggcaggcgt aatgggtatg cctttgtagg
    44821 cctgataaga cgcgccagcg tcgcatcagg cgataaatgc ctgatgcgac acagagattt
    44881 actcttcgag cgaatacggc agtggctcga tacacaatgt attcgcatcg tcgcgcacgc
    44941 ggaagatgct gtccggttcc atatcgttat tcatcactac ctgcaccacg acctgaccat
    45001 cttccagttt taccgcagcc agcaccgtac cagtacgacg ccagttctcg cccattttca
    45061 gctctaagtc ttcaccagct tccggcagtc ggctggcact acctgtcagc aaccagagcg
    45121 cgcgtttgtt ggcaccacgg aattttgctc gcgccaccat ctcttgtccg gtatagcagc
    45181 ctttcttaaa gctgataccg cccagcgcct ggaggttggt cgcctgtggg ataaactgcc
    45241 cgctgttcgc ggcatcaatc accgggaaac ccgcttcaat gtttaatgcc agccactgtt
    45301 ggctattgtt cagttccgct tcaccgcgca gtttatcggt cagcatattg gcagtagctt
    45361 catcggttac gatcaggaaa cgttctgccg ggtgttcaaa ccatagcaaa gtggtcgcgc
    45421 cttctttaac tacctgtttt tctttcgaag gcagttcact aaagatattt gccagcgcgg
    45481 cgcgcgcctg aaaaccggca acaccaagca gcacacgctc gtcgtctggc gcgatggtca
    45541 ctttagagaa caccgcatat tttttcagtt cagccagctg cggttcacgc acgctgcgcc
    45601 gttcaatcca tgcaaagcca tcgccgtcgc ggaacagacg taaattgctc cacattttac
    45661 ctttggcgtc gcaatgggcg gcgagcaggt gctgatcatc tgtcatctgg ctgacatctg
    45721 ctgtcacctg gccctgcaaa tatttttcgc tgtccgcgcc agtaatggtg gcaagcgccc
    45781 agtcatcaag cgtcatcagc gtcagtggca aacgggcaga agccgtaggc tgacggggag
    45841 gaaaaggtgt aaaagccata atagagtcct gagttgctta acggcgtatt aataactaat
    45901 ggtaaaagag ctatgaggca atgcaagtgc tttcactacg cgatctgtgc cttctgcgag
    45961 gcgacttccc aaaaaatgaa gcccttaaat tgttgtgaat taatttcgcg atcgcgattg
    46021 tgtttactga tattcccgta aaagaagtcg aaaaacacgt tacaatagcc gggtactgga
    46081 ttttcgtgag acaggaagaa gatggacatt aacaacaaag cccgcattca ttgggcatgc
    46141 cgccgtggta tgcgcgaact cgatatttca atcatgccgt ttttcgaaca tgagtatgac
    46201 agcttaagcg atgacgaaaa acgcatcttt attcgtctgc tggaatgtga cgatcccgat
    46261 ctgtttaact ggctgatgaa tcacggtaaa ccggccgatg cagaactgga aatgatggtc
    46321 cgactcatcc agacacggaa ccgggaacgt ggtcctgtgg caatctgatt tgcgcgtctc
    46381 ctggcgcgca cagtggcttt ccttgctgat tcatgggctg gttgccgctg ttattttact
    46441 catgccctgg ccgctcagtt acaccccgtt atggatggtg ttactttcgc tggtggtgtt
    46501 tgattgcgtt cgcagccagc ggcgtattaa tgctcgccag ggggaaattc gcttgttgat
    46561 ggacgggcgt ttgcgttggc aagggcagga gtggagcatc gtcaaagcgc cgtggatgat
    46621 taagagcggc atgatgctgc gtttacgttc tgatagcggt aaacggcaac atttatggct
    46681 ggcagccgac agcatggacg aagccgaatg gcgggattta cggcggattt tgttgcaaca
    46741 agagacgcaa agataaagat gacggcaaag catatgcgct aagccgtcag tgatatcagg
    46801 cgtaatgctc tgccatttcg ttgaggattt gctcgcacca gctctgaata cgctcgtcgc
    46861 taaggtcata ctggttagtt tcgtccagcg ccagccccac gaacagttgc ccgtcagcaa
    46921 tcaccggttt cgggctggta aattcatacc cttccgttgg ccagtagccg acgaacttca
    46981 cgcctttggt cgagagtttg tcatgcagca taccgagcgc atcgaggaac cactcgccgt
    47041 atcccagttg atcgccaagc ccatacagcg caacaatttt accttcgagg ttcaggtcgt
    47101 cgagctgatc ccagacggct tcccagtctt cctggatttc gccaaaatcc caggtcggga
    47161 tacccagaat gagcacatcg tactgctcca ttaatttcgg ggagtcgtcc ttgaggttat
    47221 gtaaggtcac cagttctggg ccgataatat cgcggatttt ttctgccgcc atttcggtgt
    47281 aacaggtgct ggaaccgtaa aaaagaccca tattcataac gtaagcttct cgattctgct
    47341 gtggcttttg cggctagtgt atcagaatcg ctttagtcac ggcacaataa ggcataatgg
    47401 atcaaatgag tgaccataag gggcgcaagt gaaacaggat ctggcacgca tcgagcagtt
    47461 tcttgatgct ctgtggctgg aaaaaaatct ggctgaaaat acgttgaacg cttaccgtcg
    47521 cgatctgtca atgatggtgg agtggttgca tcaccgcggg ttgacgctgg cgacggcgca
    47581 aagtgacgat ttacaggcat tactggcaga acggctggag ggcgggtata aagccaccag
    47641 ctcagcgcgt ttgctgagtg cagtgcgccg attgttccag tatctttatc gcgaaaagtt
    47701 tcgtgaagat gatcccagtg cgcatctcgc ttcaccgaaa ttgccccagc gtttgccaaa
    47761 agatttaagc gaagcgcagg tcgaacgttt attacaggca ccattaattg atcagccact
    47821 ggagctacgc gataaagcta tgcttgaagt gttgtatgct accgggctgc gtgtctctga
    47881 actggtcggg ctgacaatga gtgatatcag cctgcgtcag ggcgtagtgc gggtcattgg
    47941 taaaggcaac aaagagcgcc tggtgccgtt aggtgaagag gcggtttact ggctggaaac
    48001 ctatctggaa catgggcgtc cgtggctact gaatggtgtg tcaattgacg tgttgtttcc
    48061 cagccagcgt gcgcagcaga tgacgcgaca gaccttctgg caccgcatta aacattatgc
    48121 cgtgctggcg ggtatcgaca gcgaaaagct gtcaccgcat gtgttgcgtc acgcttttgc
    48181 cactcactta ttaaatcatg gtgcggattt acgcgtggtg cagatgctac tgggccacag
    48241 cgatctctcc accacgcaaa tttatacgca tgtcgctacc gagcgtctgc ggcaacttca
    48301 tcaacagcat cacccgcggg cgtgatgtct gaaaagaacg ggaagattta tgaagaaagg
    48361 ttttatgttg tttactttgt tagcggcgtt ttcaggcttc gctcaggctg atgacgcggc
    48421 aattcaacaa acattagcca aaatgggcat caaaagcagc gatattcagc ccgcgcccgt
    48481 agctggcatg aagactgtgc tgactaacag cggtgtgttg tacatcaccg atgatggtaa
    48541 acatatcatt caggggccaa tgtatgacgt tagcggcacg gctccggtca atgtcaccaa
    48601 taagatgctg ttaaagcagt tgaatgcgct tgaaaaagag atgatcgttt ataaagcacc
    48661 gcaggaaaaa cacgtcatca ccgtgtttac tgatattacc tgtggttact gccacaaact
    48721 gcatgaacaa atggcagact ataacgcgct ggggatcacc gtgcgttatc ttgctttccc
    48781 acgccagggg ctggacagcg atgctgagaa agaaatgaaa gctatctggt gtgcgaaaga
    48841 taaaaacaaa gcgtttgatg atgtgatggc aggtaaaagc gtcgcaccag ccagctgcga
    48901 cgtggatatt gccgaccatt acgcacttgg cgtccagctt ggcgttagcg gtactccggc
    48961 agttgtgctg agcaatggca cacttgttcc gggttaccag ccgccgaaag agatgaaaga
    49021 attcctcgac gaacaccaaa aaatgaccag cggtaaataa ttcgcgtgaa acaacagata
    49081 caacttcgtc gccgtgaagt cgatgaaacg gcagacttgc ccgcggaatt gcctcccttg
    49141 ctgcgccgtt tatacgccag ccggggagta cgcagtgcgc aagaactgga acgcagtgtt
    49201 aaaggtatgc tgccctggca gcaactgagc ggcgtcgaaa aggccgttga gatcctttac
    49261 aacgcttttc gcgaaggaac gcggattatt gtggtcggtg atttcgacgc cgacggtgcc
    49321 accagcacgg ctctaagcgt gctggcgatg cgctcgcttg gttgcagcaa tatcgactac
    49381 ctggtaccaa accgtttcga agacggttac ggcttaagcc cggaagtggt cgatcaggcc
    49441 catgcccgtg gcgcgcagtt aattgtcacg gtggataacg gtatttcctc ccatgcggga
    49501 gttgagcacg ctcgctcgtt gggcatcccg gttattgtta ccgatcacca tttgccgggc
    49561 gacacattac ccgcagcgga agcgatcatt aaccctaact tgcgcgactg taatttcccg
    49621 tcgaaatcac tggcaggcgt gggtgtggcg ttttatctga tgctggcgct gcgcaccttt
    49681 ttgcgcgatc agggctggtt tgatgagcgc ggcatcgcaa ttcctaacct ggcagaactg
    49741 ctggatctgg tcgcgctggg gacagtggcg gacgtcgtgc cgctggacgc taataatcgc
    49801 attctgacct ggcaggggat gagtcgcatc cgtgccggaa agtgccgtcc agggattaaa
    49861 gcgctgctgg aagtggcaaa ccgtgatgca caaaaactcg ccgccagcga tttaggtttt
    49921 gcgctggggc cacgtctcaa tgctgccggg cgactggacg atatgtccgt cggtgtggcg
    49981 ctgttgctgt gcgacaacat cggcgaagcg cgcgtgctgg caaatgaact cgatgcgcta
    50041 aatcagacgc gaaaagagat cgaacaagga atgcagattg aagccttgac cctgtgcgag
    50101 aaactggagc gaagtcgcga cacgctaccc ggcgggctgg caatgtatca ccccgaatgg
    50161 catcagggcg ttgtcggtat tctggcttcg cgcatcaaag agcgttttca ccgtccggtt
    50221 atcgcctttg cgccagcagg tgatggtacg ctgaaaggtt ccggtcgctc cattcagggg
    50281 ctgcatatgc gtgatgcgct ggagcgatta gacacactct accccggtat gatgctgaag
    50341 tttggcggtc atgcgatggc ggcgggtttg tcgctggaag aggataaatt cgaactcttt
    50401 caacaacggt ttggcgagtt ggttaccgag tggctggacc cttcgctatt gcaaggcgaa
    50461 gtggtgtcag acggcccgtt aagcccggcc gaaatgacca tggaagtggc gcagctgctg
    50521 cgcgatgctg gcccgtgggg gcagatgttc ccggagccgc tgtttgatgg tcatttccgt
    50581 ctgctgcaac agcggctggt gggcgaacgt catttgaaag tcatggtcga accggtcggc
    50641 ggcggtccgc tgctggatgg tattgctttt aatgtcgata ccgccctctg gccggataac
    50701 ggcgtgcgcg aagtgcaact ggcttacaag ctcgatatca acgagtttcg cggcaaccgc
    50761 agcctgcaaa ttatcatcga caatatctgg ccaatttagc gtcatcttct ctataaaaaa
    50821 gagcgtggat tgggtacaat cccgctctta tcaccgcatt ttgactagct caataaaaga
    50881 aatcagacca tgtttgaaat taatccggta aataatcgca ttcaggacct cacggaacgc
    50941 tccgacgttc ttagggggta tctttgacta cgacgccaag aaagagcgtc tggaagaagt
    51001 aaacgccgag ctggaacagc cggatgtctg gaacgaaccc gaacgcgcac aggcgctggg
    51061 taaagagcgt tcctccctcg aagccgttgt cgacaccctc gaccaaatga aacaggggct
    51121 ggaagatgtt tctggtctgc tggaactggc tgtagaagct gacgacgaag aaacctttaa
    51181 cgaagccgtt gctgaactcg acgccctgga agaaaaactg gcgcagcttg agttccgccg
    51241 tatgttctct ggcgaatatg acagcgccga ctgctacctc gatattcagg cggggtctgg
    51301 cggtacggaa gcacaggact gggcgagcat gcttgagcgt atgtatttgc gctgggcaga
    51361 atcgcgtggt ttcaaaactg aaatcatcga agagtcggaa ggtgaagtgg cgggtattaa
    51421 atccgtgacg atcaaaatct ccggcgatta cgcttacggc tggctgcgta cagaaactgg
    51481 cgttcaccgc ctggtgcgta agagcccgtt tgactccggc ggtcgtcgcc acacgtcgtt
    51541 cagctccgcg tttgtttatc cggaagttga tgatgatatt gatatcgaaa tcaacccggc
    51601 ggatctgcgc attgacgttt atcgcgcgtc cggcgcgggc ggtcagcacg ttaaccgtac
    51661 cgaatctgcg gtgcgtatta cccacatccc gaccgggatc gtgacccagt gccagaacga
    51721 ccgttcccag cacaagaaca aagaccaggc catgaagcag atgaaagcga agctttatga
    51781 actggagatg cagaagaaaa atgccgagaa acaggcgatg gaagataaca aatccgacat
    51841 cggctggggc agccagattc gttcttatgt ccttgatgac tcccgcatta aagatctgcg
    51901 caccggggtt gaaacccgca atacgcaggc cgtgctggac ggcagcctgg atcaatttat
    51961 cgaagcaagt ttgaaagcag ggttatgagg aaccaacatg tctgaacaac acgcacaggg
    52021 cgctgacgcg gtagtcgatc ttaacaatga actgaaaacg cgtcgtgaga agctggcgaa
    52081 cctgcgtgag cagggaattg ccttcccgaa cgatttccgt cgcgatcata cctctgacca
    52141 attgcacgca gaattcgacg gtaaagagaa cgaagaactt gaagcgctga acatcgaagt
    52201 cgccgttgct ggccgcatga tgacccgccg tattatgggt aaagcctctt ttgttaccct
    52261 gcaggacgtt ggcggtcgta ttcagctgta cgttgcccgt gacgatcttc cggaaggcgt
    52321 ttacaacgag cagttcaaaa aatgggacct cggcgacatc ctcggcgcga aaggtaaact
    52381 gttcaaaacc aaaaccggcg aactgtctat ccactgtacc gagttgcgtc tgctgaccaa
    52441 agcactgcgt ccgctgccgg ataaattcca cggcttgcag gatcaggaag cgcgctatcg
    52501 tcagcgttat ctcgatctca tctccaacga tgaatcccgc aacaccttta aagtgcgctc
    52561 gcagatcctc tctggtattc gccagttcat ggtgaaccgc ggctttatgg aagttgaaac
    52621 gccgatgatg caggtgatcc ctggcggtgc cgctgcgcgt ccgtttatca cccaccataa
    52681 cgcgctggat ctcgacatgt acctgcgtat cgcgccggaa ctgtacctca agcgtctggt
    52741 ggttggtggc ttcgagcgtg tattcgaaat caaccgtaac ttccgtaacg aaggtatttc
    52801 cgtacgtcat aacccagagt tcaccatgat ggaactctat atggcttacg cagattacaa
    52861 agatctgatc gagctgaccg aatcgctgtt ccgtactctg gcacaggata ttctcggtaa
    52921 gacggaagtg acctacggcg acgtgacgct ggatttcggt aaaccgttcg aaaaactgac
    52981 catgcgtgaa gcgattaaga aatatcgccc ggaaaccgac atggcggatc tggacaactt
    53041 cgactctgca aaagcgattg ttgaatctat cggtatccac gttgagaaga gctggggtct
    53101 gggccgtatc gttaccgaga tcttcgaaga agtggcagaa gcacatctga ttcagccgac
    53161 cttcattact gaatatccgg cagaagtttc tccgctggcg cgtcgtaacg acgttaaccc
    53221 ggaaatcaca gaccgctttg agttcttcat tggtggtcgt gaaatcggta acggctttag
    53281 cgagctgaat gatgcggaag atcaggcgca acgcttcctg gatcaggttg ccgcgaaaga
    53341 tgcaggtgac gacgaagcga tgttctacga cgaagattac gtcaccgcac tggaacatgg
    53401 cttaccgccg acagcaggtc tggggattgg tatcgaccgt atggtaatgc tgttcaccaa
    53461 cagccatacc atccgcgacg ttattctgtt cccggcgatg cgtccggtga aataagcatt
    53521 acgttatgcg cataaccccg gcagatgtcg gggtttttta attgtgctgc gcgaaagcag
    53581 acaacaattt tcttgcttca ctattggctg cctgcatcac catccacgga ctgaacgccc
    53641 acggcgtggc atcaataccg tgtaatacat ctgctaaatc acaccattga taatccatca
    53701 cttcatcatc gttgatctgt aacgcactgg tggtgcgtgc ggcaaatacc ggacacactt
    53761 cattttccac aatgccattc ggatcggtgg cgcgatagcg aaagtcagga tagatagatt
    53821 caggaggcgt aatttccacg ccaagctcat aacggcaacg gcggatcacc gcgtcttcgt
    53881 tgctttctcc cagttgtggg tgcccacaaa ccgagttagt ccacacgcca ggccatgctt
    53941 ttttgctcag tgcgcggcgg gtaactaata attgtccttt ggcattaaac agccaactgg
    54001 agaacgcgag atgtaagcgg gtgtctgccg tgtgtgcggc atacttttcc agcgtacccg
    54061 tgggaactcc ctgtgcattc aataaaatga cgtgttccgt ttgcataatt tctcacatgt
    54121 aattctgatc actcatcatt atagataatt gaagagtgaa atgattaacc aagcgagcgt
    54181 ttatcgacaa ccgcccgttt tgagcggatg ccgacaggta aaaaccattt aaaaaagtag
    54241 catcatatat ttgaagcgac tttcaatggc cattgcaaca cagtcagaaa tgacaggaat
    54301 agagagagta aaaagcaaca gcagactgaa tatagcgcgc attaaaaaat tcatattatt
    54361 ctccatgctc atttttcttc agcttgcgca tcagaagcca catacgtact gtcctgactt
    54421 ttaaatcttt atcagagacg gattccttca ccaggtcagc atgatgctga tagccattaa
    54481 agaagacgtt cagcacaacg gcgctaaggg ttgcgagcat aatgccgcta tgtagcagcg
    54541 gttgcagtac ggccggtaac ttagaaaaga aatcgtgaga gagcgttgga gtcatgccaa
    54601 cgccgagact gatcgccaca atatagaggt tgtaacggtt ggtggtgtag ttacagcgcg
    54661 acagaattcg aatccctgtc gccagtacca tgccgaacat caccagacca gcgccgccca
    54721 gcacaaattg cggaatggag gctaccagca ccgccatttt tggcaccatg ccaaacagga
    54781 ttaatataat tcccgaagaa atacacaccc agcggctatg aacgcgcgtc acgctaacca
    54841 ggccaacgtt ttgagaaaat gacgtgtggg ggaagctgtt aaacgtgcca ccaatcattg
    54901 tccctacgcc atcgacacgc agcccgcgaa taatatcgtg cgaagagagt ttacgaccga
    54961 ctatttcacc cagtgccagg aacatcccca tcgactcgat aaacacgatg attaacacgg
    55021 cagtcatggt cagaatggaa acgggatcga aaatcggcat accaaacgac atcggggtga
    55081 caatcgcaaa ccacgaggca tcatgtagcc cggataaatt gacttcattc atcatccacg
    55141 aaagtaaaaa gccaaataca atccccagta atacggcgac gttggacata aatcctttcg
    55201 catagcgagt aatgagcaag ataaaaatta agacggcaaa ggagatacct aaataaacgg
    55261 gattaccata ttgcggattc cctttacctc cggcggccca gtcaataccc acctgaatga
    55321 tgctaagccc gatagaagta ataaccacac cggtaaccag tggcgggaat aaaggcatca
    55381 agcgaccgat aagttgcgct aataatgtgg tgataaaacc cgcggcgata gtggcaccaa
    55441 atatacccag caggccgata tccgggttca tcccaatggc tatcattggt gttacagcag
    55501 caaaggtcac cgacataatc accggcaggc ggatccccat aaagcggccg ataccgatac
    55561 attgcaataa tgtgacgatc ccgcagcaaa agagatccga gctaatgagc atcgcaatag
    55621 cttcttttga gaggcccagt cggtcaccaa tcataagagg aacagcgact gcacctgcgt
    55681 acatgacaag gacgtgttgc agaccgagta tgatcagctt tcctggtgat aatatgcggt
    55741 caacctcatc agttgggcgg tcttgcccag aagatgaggg aagttgggaa tctatggcgc
    55801 tcatcagtgt ttctcctttc gcccgccaga gtaaaaagca accgtttacg ttaatgaaac
    55861 tgtctaaaca catgctcgtg gagtgccagt ttttgcgagg aaggggagtt agagacagga
    55921 attgcggagg caatcacata atcgtgctta aactacgaaa atgacaagag aacctgacaa
    55981 tggaattatc tgtaattttc tcatattgat atttcattaa tcattctggc atgtcgcact
    56041 ctcgcattta atcgttttta tctggatagc gctatttggg gcggcaatgt tgaaataatg
    56101 aaagcggcat agattgaggg gcaataattt gaactcagtc gcaaaattat aattaaaacg
    56161 gtcatgctac ctaatatatg cccgtttatt gcatcgcgaa atttgagaag acataactca
    56221 ttaaatgaaa gggatgcaaa tgaataagtt tatcgctgct gaagccgcgg aatgtatagg
    56281 ctgtcatgct tgtgaaattg cctgtgcggt ggcacacaat caagaaaact ggccgctgag
    56341 tcacagtgac tttcgaccac gtatccacgt tgtagggaaa ggcctggctg cgaatccggt
    56401 ggcctgccat cactgcaaca atgccccttg cgttacggct tgtccggtta atgctctgac
    56461 tttccagtcc gatagcgtac aactggacga gcaaaaatgt attggttgta aaagatgcgc
    56521 aatcgcttgc ccctttggcg tcgttgagat ggtcgatacg attgcgcaga aatgcgacct
    56581 ttgtaaccag cgcagttccg gcacgcaagc ctgtattgaa gtttgcccaa cacaggcgtt
    56641 acgactgatg gacgataaag ggttacagca gataaaggtg gcccgccagc gcaaaacggc
    56701 agcaggaaaa gcgtcatcag acgctcagcc atctcgcagt gcagcgttgc tccccgttaa
    56761 ctcgcgtaaa ggcgcagata aaatttcagc aagtgaacgg aaaaaccact ttggcgaaat
    56821 ctattgcggg ctggatccac aacaagcgac ttatgagagt gaccgctgtg tttattgtgc
    56881 cgaaaaagct aactgcaact ggcattgccc gctgcataac gctattccgg attacatccg
    56941 tctggtacag gagggaaaga ttattgaagc ggcagaactt tgccaccaga ccagttcctt
    57001 accagaaatc tgcggcaggg tatgtccaca ggaccgtctt tgtgaaggcg catgtacttt
    57061 gaaagatcac tctggcgcag tctctatcgg taatctggaa cgctacatca ccgataccgc
    57121 gctggcgatg ggctggcgtc ccgatgtcag caaagttgtt ccccgtatcg agaaagtggc
    57181 ggtgattggc gctgggcctg cagggttagg gtgtgctgat attctggcgc gcgcaggagt
    57241 tcaggtcgat gtctttgatc gccatccaga aattggcggt atgctgactt ttggcattcc
    57301 tcctttcaaa ctcgataaaa cggtattaag ccagcggcga gagatattca ccgcaatggg
    57361 aatcgacttt catcttaact gtgaaattgg ccgcgatatt acctttagcg atttaacttc
    57421 tgaatatgat gcagttttca tcggcgtggg gacttacggg atgatgcgag cagatctgcc
    57481 gcatgaagat gcgcccggtg tcattcaggc tctaccgttc ctgactgccc atacccgcca
    57541 gctcatggga ttgccggagt ctgaagagta tccgctgacg gacgtggaag gtaagcgagt
    57601 cgtggtattg ggcggtggcg atacgacaat ggattgtttg cggacttcca ttcgcctcaa
    57661 tgctgccagc gtgacctgcg cgtatcgtcg tgatgaagtc agtatgccgg gctcgcgcaa
    57721 agaggtggtc aatgcgcgcg aggaaggtgt cgagtttcaa ttcaatgttc agccgcaata
    57781 tatcgcttgt gacgaagatg ggcgtttaac tgcggtgggc ctgattcgta ccgccatggg
    57841 tgagccgggg ccggatggtc gccgtcgtcc tcgtccggtt gcgggttcag agtttgaatt
    57901 gcccgccgat gttctcatta tggcctttgg tttccaggcg catgccatgc cgtggttgca
    57961 gggcagcgga attaaactcg ataaatgggg cctgattcaa accggtgacg tcgggtattt
    58021 acctacccag acgcatctga aaaaagtctt tgctggtggt gatgcagttc atggcgcgga
    58081 tctggttgtc actgcaatgg ccgcaggaag gcaggcggcg cgcgatatgt taactctgtt
    58141 tgatacgaag gcatcgtgat gaaatcgtta attatcgtta atccggctga ctgtattggc
    58201 tgccgtactt gtgaagtggc ctgtgtggtc gctcaccctt cataacagga gttgaatgcc
    58261 gatatctttt taccccggct gaaggtacag cgactggact gcatcagtgc gccggtgatg
    58321 tgccatcagt gtgaaaacgc cccttgtgtt ggcgcttgcc ccgtgggggc gctgacgatg
    58381 ggcgagcagg tggtgcagac caattctgcc cgttgtattg gctgtcagag ttgcgtcagt
    58441 gcgtgcccgt ttggaatgat caccattcag tcgttgccgg gggatacccg gcaacaaatc
    58501 gtgaaatgcg atctctgtga acagcgggaa gaggggcctg cctgcgttga atcttgccca
    58561 acacaggcgc tgcagttgct tactgaaaga gaactcaggc gagtccgcca gcagcgtatt
    58621 gttgccagcg gtgagaatcc tctgtgaaag aaatctccgc gctgaaaggc gcggagaatc
    58681 tattagattg cccaaccacc cgcgtaaaat gcgaccagtg cggcagtaat gatcaccgta
    58741 ccaatattca gcttttgcca ttcgcgtgca aagacgcggc ctacgaccag tgtcacaaag
    58801 cccagcataa taccggtaac gatattacaa gtcagaacga tgaacacggc acacaccagg
    58861 ccagccatag cgtcaataaa atcattgaaa tccagcttcg agacgttact taacatcagc
    58921 aaacctacgt acatcagagc gggtgcagtg gcgtaaccag ggatcaaaaa tgacagcggt
    58981 gataagaaca gaatcagcag gaataacgcc cccactacgg ttgcggttaa ccctgtttta
    59041 cccccggcgg cggttcctgc cgctgattcg atataaaccg ctgcgggcgc tgcgcccacc
    59101 aggccggaga atattgaact tactgagtca ctggtcaggg ctttgccgcc gttgatgatc
    59161 tggttgtctt tatccaacaa attcgcctga ccggcgacgg cacggatggt gccagtagcg
    59221 tcgaacactg cggtcatcac caatgccagt acactcggaa gtacagttgg ctggagtgcg
    59281 cccataatat cgaggctgaa aatcagagac ttaccatctt cgccagtcag gcttggcatc
    59341 gccaccagac cgtggtattt caccgctggg tcaaagatta agccgatgat cgaaattgca
    59401 ataatcacca acaagatacc gccgggtacg cgacacttct ccagaccgaa gatgaccgcc
    59461 agccccagca agctcatcat caccgggaag gaggtaaacg caccgagcgc cactggcaag
    59521 ccttcaatcg ggtttttgat aaccataccc acaccgttag cagcaatcag cagcagaaac
    59581 agcccgatac cgatacctgt accgtgagcg atacccatcg gcaaattacg taagatccag
    59641 gtacgcacac cggttacgga aatggcggtg aagatgacgc ccatcagaaa taccgcgccc
    59701 agtgcgacag gaacgctaat ttgttgcccg agtaccagac tgaatgcggt aaacgccgtc
    59761 aaggaaatcg cgcaaccaat cgccattggc aaattagccc ataaccccat cagcaacgag
    59821 ccgaagcccg cgaccagaca ggtggcaaca aacacagctg cgggaggaaa acctgctttg
    59881 cccagcattc ccggaacgac gataacggaa taaaccatgg ccagaaaggt cgttaagcca
    59941 gccagtactt cctgacgaac ggtactgcca cgagcggtaa ttttaaaata attatccagc
    60001 gcgccctgtg gctttggtgc gtccggtgtt tgtaggatgt ctccagacat aatgatgtcc
    60061 tcacgaggat gtaaaagttt tattattagt tgcgttcgta caccagacga ccatcaacgt
    60121 aggtgcggta gatcgaacgg tcatcgccca acgtcatcat cacgaacaat ttgtcgacta
    60181 aagaaacaga gttgtcatag cgcagctgct gtagcggagt ggcggtgggt tccatcacca
    60241 cgaaatcagc ctctttgcca ggtaaaaagt tgccaatcaa atcgtcaagg cccagagatt
    60301 tcgctccgcc gagcgtggcc aggtaaaacg cttcatatgc cgagaggcga tagccttgta
    60361 attgcaacac tttgtaggct tcgttcagcg tttgcagcat gttgaaagtg gttccggcac
    60421 cgatatccgt tcccatgccc actttaactt tcttctgcca tgcttttttc aagttgaata
    60481 acccgctgcc gaggtaaagg ttggaggtcg gacagaaagc aatgctggat ttggtttcgc
    60541 tgagacgatc ccactctttt tctttgagat ggacgcagtg agcaaagaca cagtttttac
    60601 cggtcaggcc gtactgatgg taaacatcca gataaccatc atggtcagga taaagcgatt
    60661 tcacccaggc aatttcatct ttgttttcac agagatgggt atgtacccac gtatccggat
    60721 attcctcttt caggcgttgc gccatcgcca tctgttcagg agaagaagta ggggcgaagc
    60781 gtggcgtaat cgcatatagc agacgaccat ttttgtgcca gcgttcaatc agttctttgc
    60841 tttggtgata gctgctttcg gcagtgtcta gcagataatc cggtgcgttg cggtccatca
    60901 tcaccttacc ggcaatcata cgcatattga tatgactggc ggcttcaaac agcgcatcaa
    60961 cggattgcgg atgaacagtg ccaaacacca gcgccgtggt ggttccgtta cgtaaaagct
    61021 gcttgatgaa gaacgccgac atttcgcggg cgtactctaa atcctcataa cgacgttcag
    61081 tagggaaggt gtgtttattc aaccactcca gcaattgttc cccataggcc cccaccattt
    61141 cactttgcgg ataatggata tgtgtatcga caaagcccgg tactatcagt ttgccgcgat
    61201 agtcgcgcac gcgaatagtg tcaggaattt gatgctttcc gtcttcccat tcgccaaacc
    61261 attccacttt tccctgttta atgagtaata aaccatcctc aataaaccgc agcgcagagg
    61321 caatctcttc cgggttatcg accgtacggg cgacatcaat aaaactgcct cgtaccgctt
    61381 ttaacgtgtg ttctcctgac atcattgact cctttactgt taatccatct cttccgctga
    61441 ggtaatacca ggcagaacgt tttcctgtcg gtagccacca gggaggataa tatttaataa
    61501 aatcgcagtt aacccgccag cacaaatagg gttttcaact aatacataaa tagaggctgg
    61561 caatatttta aaaatttcgg gatcgtagga gacgccaagt cctaaaccta aagaagtggc
    61621 gacaataagt gtttctcggc gctttaaacc gttggtgatg atgatgcgaa tcccagcgat
    61681 ggcaatcatg gaaaacatca acgtcattgc gcctcccaga actgccgagg gaatggtcgt
    61741 gaagaagccg ccaatcatcg gaaacaaacc gaggataacc agcattaccg cgatggttcg
    61801 cccgacataa cgtgaagcga cgccagtcat ctgaataacc ccattatttt gcgcaaacgt
    61861 ggttaagggt aatgacccga cagcggaggc gataacagaa accagaccgt ctgccagcac
    61921 gccgcctttc agtcgggact gatactcttc tccctgaatg ggtcggcggg aaaccattgc
    61981 cgtggcggtg atatcgccga cagcttccag cacgctaagc agataaatcg tgccaaccac
    62041 caggaactga tggaagctaa aactaaagcc gtatttgaac ggatgtggga tggtgattaa
    62101 cggcaaattg cgcatactgc tgaaatccac catgcccagg cataacgacg cgatataacc
    62161 gacgcatagc ccaatggcga tccctcccat gcgtagcaac ggactgcgac agcagttaaa
    62221 gccgatcacc acaatcaaaa ccaataaacc aacgccgaga tgttcgtaat tgccgaaggt
    62281 accgctgctt ttggcagcaa atcctccacc aaaatcgata atgccgactt taatcaggct
    62341 taagccgatc atcagtacca caataccgct gacagtaggc gtaataaccc gacgtaaata
    62401 gggcaagata aatgaagatc cgacaaccag aaatgcgcca acgaaggaga cgccgaggag
    62461 cgacgacatg attaactctt cgtgaaaacc gtcgcttttc atgctgctgc ccagcgcaat
    62521 catgaccgta acaaatgaaa aattgactga ctgaattgag agtaggccag aaccgacgat
    62581 gccatagcgg tttacttgta accaggtacc aataccagag gcgatcatcg ccattgaaac
    62641 aagataggca gttgtttcag cggaaagctg taaggccgcc cccacgatta acgcgggggt
    62701 taccatcgga acgaaaattg ccaacagatg ggtaatggca ccaacgagag cctgatgaaa
    62761 gggagggcga tcctccagtt caaatataag gtcagaacct gcatggttta tatcagacat
    62821 cccttcactc cttttaaagt aaatttgatc atctgaatta gtaaaataaa ttcccacgaa
    62881 tttggggcgc aggacgcctt gagaacggct tatctcattt tcgcagtcac tatttagtca
    62941 tcgtccaaac agagaaacag gccgccgaag cggccattta ttatttatat cttttccagc
    63001 gcggtgagta ttttctccgg tgtgaaatgc cattcgcgta accagatgcc gcatgcatcg
    63061 tgaattgcgg tagcaatcgc cggagctgcg ccatttacac cgatttccga gatcgatttc
    63121 gccccgaacg ggccgacttt atcgtcgctc ggcaccagca ctgcgcggaa atcacgcgga
    63181 atgtcaccaa ttttcggtgc gccgtagctg cgtaaatcac gcgttaacgg gtgaccttcg
    63241 gcgtcataaa tgatctcttc gctcatactg tggccgatag ctcgcagggt agcaccgtag
    63301 atttgtccca acgctaactc tggattgacc ggtgtaccgc agtccagcaa ggcgtagaat
    63361 ttatccaggc ggatttcacc cgtacgcgtg ttgacggcaa cttcagcgaa gtttgcgcca
    63421 tacgggaagg cgaaatcagg cgtgatataa ctgccagttc ccaccagtga accaaagccg
    63481 gtgccggttt cgcctttatg ggcaatatcc ccgaaactaa cttcgccttt cttgccgcgc
    63541 acgacgcccg gcgttgctag ttgaacatct gccactggct cacccaacat ttgcgcgccg
    63601 tggaacagaa ttttctcccg cagattttcc gctgccaaac gcgccgcgtt acccgagaag
    63661 caagtaccgg acgaggcata tgcgccttta tcaaacaacg catgatcggt atcaccggag
    63721 ataacatgca cgtcctgcgg tgggcagtgc agcacttctg ctgccagttt cgtcaccacg
    63781 gtatccagac cagtaccaat atccgcaccg ccagaatgaa cgataaaggt accgtctgat
    63841 tccagtttga tcatgcagtt agcctgatcg atatccggga tccctgattt ctgcatgata
    63901 atggcgacac cacgaccgat gtgccagtca ccattttgtg gttttggtga agaccattgg
    63961 atcatctcgc gcccctgacg caggatctct tccagtgcgc agctggcggc ggaaggaacg
    64021 gaggtcggcg ctttaccttc accgattgca ccgagaattt tcagctcttg cccttcgtgt
    64081 acccggttac gttcgataat ttccagttgg tcgatctgta actgttcagc cagttccgct
    64141 aatgccatgg tgatagcgaa gttacctttc ggtgcgccat aaccctgata agcaccattt
    64201 gggcaaatgt tgctgtagta ggtggtgacc tggaaatcga cgttatcgca cggatataac
    64261 ggcagcgaca gcgccggtcc gttacacggt acggtgagtg agtggttgcc gtaagggcca
    64321 gtgttggcgc ggaaatccat cttcactgcc gtcaggcgac catctttttt cgctcccagt
    64381 ttgacggtga ctttcgcgac gtgacgagag gtgttagcaa taaactcttc ttcacgggtg
    64441 tagcggaaca gtaccggacg cccggtcacg caggttgccc aggcgcacac ttcttccagc
    64501 aggatgtcct gtttggaacc aaaaccgccg ccaactcgct ctttaatgac atgaacttta
    64561 tgctgtttca tgtccacgag gcgcgcgacc tggcggcgta agtgccatgg tacctgggtg
    64621 gaggcgtgga taaccagacg atcgccgtcc atccgggtaa agcagatatg tgtttcagtc
    64681 gggcactgct gcgcctgcgt tgagttatag gttcgctcaa tgatcacatc ggcatcggca
    64741 aagcctttgt ccatatcgcc aatatgacca tgaatactag cggcgatatt tttgcgaggg
    64801 cgagaaccga tcgggaagtt gatgatcata tgctcgccgc gctgggctgc atggctgtta
    64861 tcgtcttcca gagtatctgg cgcaccagca acatacacca ccggttcatc gtgcacgaca
    64921 ggcgcatctt ccgccattgc ttcgtcgatc gacattaccg gcttaagcac ttcatattca
    64981 acctcgatga gtttcaatgc ttcgagcgca atttcttcac tttcagcgac caccgcagca
    65041 acgcgatcgc cgacgtgacg catttttttg ccgaacatac ggcggtcaag cggtgacggt
    65101 tccggtgcgc tctgaccccc cggggtatag tagatatcag ggcaattcag gtgagtaata
    65161 acgtgaacga cgcccggtaa ggcttcagct ttgctgacat ccagatgagt aatcagtgcg
    65221 tgagcgtgtg ggctacgtaa cattttaatg acgcaggcgt cagccgttac gcggtcttca
    65281 acatagcagg gtttcgcctg caccattttc gcggcatcag ttttaggata atgcttgcca
    65341 atgacttcaa ggtcgtcacg gaaagtcgga gcgatatcaa tggtggcctg cggattattt
    65401 ttacgtgcaa ccgccagttc aatgacctga tagtattgct gccagccagc atcgcggctg
    65461 aacaaaccag aaagcgcgtc gtcgatctct tcgcgggtag gtgcggcgat gcgatcgaga
    65521 agatcggtga taatcagagc tgcagctgga tcgttataac cagactgcac cacgccaaca
    65581 tcaaccattg cctgttgaac cagacttaac tcgttccatt tgcccagaga ttctgcggta
    65641 cgaatatctg ccttctctaa ctgtgcggca ataagcaagg acgcgttaac gatattaccg
    65701 ttaaagatga ttgcgtcaga accggcaaac ccgaaaccat catcactgtt gcgtacagag
    65761 tgcattccca tgttaaacaa cagcttttgc acgttttcgc ctggattaac ggttagctcc
    65821 tgaggcgcgc catttaaagt aaagtggatg atcatacggc ttcctcccca gcctgttggc
    65881 agtcggcata caggtcggct accagaactc ccgtgatata gcgtttatag gccacgctgc
    65941 cccgcaaatc ttcctgcggg aaaatggcgt tggcgacagc ctgttcaagt gcattgcctt
    66001 ccagattttg tttttcgaca tcatgcagac gcagtgcttt actggcgacg ccatccagcg
    66061 caattcgcat accgtcgtgg tctgtcattg caacggctgc cgtcacgacg gttaaacctg
    66121 cctgagaacg gctaattttg cgggtcgcac aggtgcgata cggatcttta atgataattt
    66181 cggttagcag gcgatcgcat gggcaggcca ggtagtcctc gattgacagc gtttcgccgt
    66241 tgccaaaaac cagttcagca tccagtgcca gcaggacggg aagcagcact gactcttcct
    66301 gccgggcggc gatttcacca ccaatggtcg actgattacg aacatggcgt gagtaaacaa
    66361 aaccgagggc ttcacgcagt gctgcaggaa taaatcgcgc atcacgcagt ggctgcaagc
    66421 gagacattgc gccaatccgc agtgcaccgt tatcccagtc aacccagtcc agttccagat
    66481 cctgtaagga aatggcaatc tttttatcgg tacgggttgg tgtagcattg agtttgctgc
    66541 ccccggcgaa ccagacggct tcatcctggt agcggcgctt cagttccagc gcctgttcga
    66601 cagagtcggg cctgaaaaat tgttcaatca tcttctttcc ttatttaacc ctcttcctgc
    66661 gggaagaggg catctgtcat ttatgccagc gcatccatcc gacgccacat actggcagcg
    66721 gcttttctgg cttgtgcata aatggaatcg caatcgaagt taaactgacg gtcttcatag
    66781 accatcacac cattgaccat cacgctgtga acgctgcctg agcccatacc gaaagcgata
    66841 tgcccggcga tattgtctgc cagcagcggc gtcggcgagt tgtaatcgca aatggttaaa
    66901 tcagctttgt aaccggcttc cagaagccca aatttcgcgc caaagttgcg gctcatcagt
    66961 tcgttaccgt tagtcagggc tttggcaaaa ctgtcaggcc acagcggacc acccgcatcg
    67021 cgatgtttaa agaaggcaaa tttcatctct tcaaacatgt ccgaaccaat gccgtccgtt
    67081 cccaacgcca gattgcggat gtcgctaagg tgatggttgt agccgacatg gttgttcatg
    67141 tttgaacggg cgttatgcac caggaacgca tcgcgctgat tgagtagggc gatgtcatct
    67201 ttcgacaagt acagcccatg agcgaccagc gttttgctat cgatgagatc gaattgcgcc
    67261 agtcgtgcca gcaggtcttt gccgtaccag tggtgactgt aggaaacgtc gtaaaggtct
    67321 tccgcagcgt gaatatgcaa accacggcct gtggcgttca cggcttcacg tagcatctcc
    67381 agaccggcat ctggcacggt aaacggcgcg tgagcaccga tatgtgcttc caccagatac
    67441 ggctcggttg ctgctttctt cgcctcatca atctgacggg cgaagcggat gttttcttct
    67501 acaccttcct gcaactcttt gatgccgttg ttacggtcag tagtttcaaa acaggtcatc
    67561 gcgcgcaggc caacttttaa aaatgcgtcg cgcaatgtgg agagcgaccc gccgatatac
    67621 gccggagagg catggtgatc gataaccgat gtacatccgc tcttaatcgc ttccagggaa
    67681 caaatcagcc cgctgtaata gagcgactct tcatcaaggg cgcgatcgag ccgccaccag
    67741 agatttttca gcgttgagat gaaatctggg ctgggggcga tgtttgccat aattccgcgg
    67801 gaaagccccg agtaaaaatg gttgtgcgag cagacaattc ccggcatcac aatccgacca
    67861 tgcatctctt tgtagctggc atcggggtag cgttgcgtca gggcatcgcc gatagcgaca
    67921 atcacatcat tttcgatagc gatatcaacg ccttcctgca ctttcgccgg gtgtaactgc
    67981 actgcggtga cattcttcag aatcaacatg attacacctc cacgcggccc agcagataat
    68041 gatgatgctg gtggacatgg ctgatgatgc ggcacatatc gttcagctcc ggtggtacgt
    68101 tgttgaactg accttcgctg tcgatgttta acacccagct ttggttattc agtcgtactc
    68161 gtacccggca atcttccaca aggaagcctg ggttgctgct gttatcaaag tcttgcgcca
    68221 ggctgaaaac ggtgattttg tctttgtatg gtttaccgtt ccacggacag aactgagcgc
    68281 agttgccgca ttcgttacag taagcgtcga ggtgcagcgt ctggaatcta ttctggaagc
    68341 ctgggaccgc aatggagacg ttggcgcggt tcgggcagac atccacacac ttgctgcaaa
    68401 cgtagttaca ttcaaggcag cgagcagctt cctgcgcgac aaacgcatca cgatcgtcac
    68461 tgttcaccag cgtgatagag atatcgcctt tacgttgata gatttccgcc ggattgacgt
    68521 tgttccagta tttatcgttc tggtgggaac ggatattttc ccgactaagg atggcatcgg
    68581 tcgcccgacg cgcggttccg acagcagcga caatggagga tggtccgcgc tgtacgtcgc
    68641 cgatcataaa gacgtcagtc agacgagttt cgccattatg gtcgacgtct ggccagccgt
    68701 ttttgtccag cggcacgccc atcgcattca gggcttcagt atcctgctgt tcaccaatgg
    68761 cggtgatcag gctgtctaca tgcagtgtta ctgtttcgtt ggtttcaacc ggacgacgac
    68821 gacctttctc atcgggttcg ccaagcgaca taacgcgcaa ggttaaggtg ccatcagcat
    68881 cgaaacgttc cggattattc aggaaacgga actccacgcc gtcgtgcaac gcttcttcat
    68941 actcttcgcg ccatgcgggc atctcttgca gtgaacgacg gtaaacgacc gttgcttttt
    69001 ctacgcctgg aacgcgtaac gccgcacgag cgcagtccat tgcggtgtta cccgccccga
    69061 caacgaccac atgtttgccc agcttgagcg ctgtaccctt gttgtattca cgcaggaagg
    69121 ggagtgattt caagacattt tggttgtcgc ccgccagttt cacaccgcta tttttatctg
    69181 tgccggtggc aatcagaaca tagtggaagt cctgattttt taactgctca acggttaaat
    69241 cgggtgagca gccatactca aatttcacgc cgtgagcggc aacaaaatcg atatcgtgct
    69301 gaattaactc agccggaatt cggaactgag gaatgatatt tttcaccacg ccgcccgcat
    69361 tggcttcgcg ttcaaacagc gtaaccggat ggcccgctct ggcaaggaag taacctgctg
    69421 ccagacccgc cggacctgca ccaatcacgg caaccggatg gcgtgaacca gaaccggctg
    69481 gtttgtgcca gcgttgctta tattcatccc aacctttttc cagcgcgact tttttcagtt
    69541 cgcggatatt cagcgcactg tcgtaatcca ggcgggtaca gttgtattgg cactggtgat
    69601 cgcaaatatg accggtaatg gcgggcagag cgttgcgttg gtagatgagt tccagcgcgt
    69661 cggcatagcg gtgttcgcca agcagacgga tgtattccgg aatatcttgc ttgatagcgc
    69721 aggcagtaac acagggggca acgtagcagt cggtcagcgg caggtcttct gccacttcaa
    69781 tacgctcttc tggcttccag tgtttctggg tgtattccat ggttaacgca tctgctgcca
    69841 gtctgttcag tcgttcgacg tcaacatggt caagtcccca ggcgtcggag ccttccagct
    69901 cgcgcatgca ggcacttaag cgcaaatagc cgccaggttt cagcaggtcg gttgccatag
    69961 taatagggcg aatacctgta tcaaaaatat cgcggatagt cagctgactg gcaccaccgg
    70021 aataagaaat gggcagtttg ccgtcaaagg cgcgagagag aactgctgca acattgatgg
    70081 agagcgggaa cagcgcacgg cctgacatat acatctcttc accaggcagt gcgcctttat
    70141 tgttgatggt gccgagagtg ttagtcagtt ttacgccaaa gccgagtgat ttttcttttg
    70201 ccagtgccat caggcgttcc agcatttcca gtgcttgcgt cagcttgagg tcgtgatcaa
    70261 atgactcttc ttttaagcct atgtaaccga aaccgcagac atcgaggatc tcacgaacac
    70321 gcgcgtaccc cagtaaggtc gggttaagtt tcacaaaggt gttgagccct ttttcttcca
    70381 gcatgtagcg gcaaatggct tcgatttcat gcggaggaca gccgtgcatg gtggagaggg
    70441 tgacgccatg caccatactg gtggggatgc gagcgggtaa ggcttgcaag ctttcgcgtt
    70501 tttcctgcaa tccgtggcgg gctaaaaatg cgtcatcctg gagtaattta ttcagcgtat
    70561 cgcgatattg cgcgaatttc ggatggtcag atgcgtccat catattgtcg atgaactgtt
    70621 gcattggcgg ttgcttaata ccttcgaggt tgtaaccgac gctcatatta aagatgaacg
    70681 atttaccgga atcagaaggt tggaacatcg cttcgagaag gtgcagggca aaccaggctt
    70741 tgaggtattc atcccaggct ttaagcaggg taaattcggt agaccattcg gtgttaaagc
    70801 actcgtcttc ggcatcaata cagggctttt ccagctccag gcggtcaaga atttggacgg
    70861 tttttagttc gatgaaacgt ccgccagtca gccaggaagt gacaatattt tgcgcgagct
    70921 gcgtgtgcgg gccagcggca gggccgacgg gagtggcaca ggtttcaccg aagacgctaa
    70981 cagttttacc ttttacaggt gagtaaaact gttgctcggg aataccaaag attgagcgtt
    71041 gttgttggta ttcatcaaat atgcgcgtca aaagttcctc aaacggaatg ggacgcataa
    71101 tatcccccat aaccctctcc ttagcttata cagatatcat tgagtgaccg gtgttgagaa
    71161 atccggtggt ttagggggat agagcaacaa tcgcaccact cttgccaggt ttggcgggaa
    71221 atataaaaaa atgtgaggcg gctcgaaact taagggcttt ttgtgaatga actcacaggt
    71281 gctaaatgag aattaaaatc actcctgaaa attattattg ccatcccata tatcactttt
    71341 tctcttttct caaaatcagt gattccttat cattatgata aagagtgctg gagggattaa
    71401 atatttgaac gcaatcgttt aactacgttt tttaaatttt agtatacctt ttttgcgctg
    71461 taataaagtc gtccggagta tcaatatcta aaattatttc tgcattttct acttcgacgg
    71521 aataatgagc tcccataagg agtgcctgac gcatattggt gatattgggg cgcttgattg
    71581 cctgcatcag gcatggtttt gacactaaaa tcggatggcc ggggatgcca ttatggagtg
    71641 gcagtattgc gccatcattt cgtaacgacc agatttttct gaaaatatcg atggtgaggg
    71701 ttggcatgtc accgtgggtg agaaaacaat gttctgtttg cactgcgggt actgcggcct
    71761 ttactgatgt tagtaaaccc tgcgcataat ctgggttgtg gataatagtg atattgctct
    71821 ggttcgcata acgttcgtgc agttcattac cacgatagcc ggtgactaaa ataattcggc
    71881 tacaaaactg caacgcattt ttgatacttg tatcaagaat tgtcccctgt tgccagggta
    71941 acatcatttt ccattgcccc attcttgatg ataatccggc agcggtaatt atacagtcga
    72001 tggctgacat agtggttcca ttatttatag gggctaataa cgcctgataa ttgcgttact
    72061 tcctgatgga ttaagttggg gataatttat cccagagagg tcataaagac tcgtgaaaag
    72121 tatagttgac ccatcggcat tattcattga tttaggtgcg cagaaacgtc ccactgtgat
    72181 ttctgttgtt ggtgctgggg ggaaaaccag cttgcttttt tggctggcag aactgctcca
    72241 ggctagcggc aggcgtgtgt taatcacgac gacaacacat atgtttatgc caacgtctca
    72301 ctggcccgtg gttttctgtc gtgatcccgc catgcttcct catgcgtctc ttacatcccc
    72361 catttcattt tgttttcaca gctggaaagc gaacctggga aaagtgcagg gatttacgcc
    72421 agaagcgatt gatgcactgg tgcaacgacc agagtgtgac gtaattctca ttgaagcaga
    72481 tggctcgcgt gcaatgccgt taaaagcgcc tgatgagcac gaaccttgca tacccaaaag
    72541 cagttgctgc gtgattgctg tgatgggagg acatattttg ggcgcgaaag tgagcacgga
    72601 aaatgtccat cgctggtcgc agtttgctga tattaccggg ttaacacctg atgcaacctt
    72661 gcaactgagc gatctcgttg cgctggttcg ccaccctcag ggggcgttta aaaacgtacc
    72721 gcaaggttgt cggcgagtct ggttcattaa ccgtttttct caatgtgaga atgcgattgc
    72781 gcaaagcgag ctccttcaac cgctgcaaca acacgacgta gaggcaatct ggctgggcga
    72841 tatacaagaa catcctgcaa tcgcgcgcag atttgtgaat tagcggcccg ggtattccgg
    72901 gttactgaaa ggatatccat tgaggttggt atgaatattt tcacagaggc tgcaaaactc
    72961 gaagagcaaa attgtccgtt tgctatggcg caaattattg atagccgagg ctcgactccc
    73021 cgccattctg cacaaatgtt agtgcgcgcc gatggctcta tcgtcggtac aattggtggc
    73081 ggaatggttg agcggaaggt gattgaagag tcgcttcagg cattgcagga acgtaagccg
    73141 cgattattcc atggacgtat ggctcgtaac ggtgcggatg ctgtcgggtc agattgtgga
    73201 ggtgcgatgt cagtgtttat cagcgtccat ggtatgcgtc cacgtctggt gttgattggc
    73261 gcggggcatg tcaaccgggc gatagcccag agtgcggcgc tattaggatt tgatattgcc
    73321 gttgccgata tttatcgcga aagcctcaat cctgaactat tccctccatc aaccacgctt
    73381 ctccatgctg agtcgtttgg tgcggcagtg gaagcactgg atattcgccc tgataatttt
    73441 gtcctgattg ccacgaataa tcaggatcgt gaagccctcg acaaactcat tgaacagccc
    73501 attgcatggt tggggttgct ggcaagtcgt cgcaaggttc agcttttcct gcgtcaattg
    73561 cgtgagaaag gcgtggctga agaacatatt gcccgtttac atgcgcccgt tggttacaac
    73621 ataggtgcgg aaacgccgca ggagatcgcc atcagcgtgc tggcagaaat attacaggtg
    73681 aaaaataacg cgccgggtgg gctgatgatg aaaccttctc atccttccgg acaccagctg
    73741 gtggtgattc gcggtgcggg agatatcgcc agtggtgtgg cgctacgtct gtatcatgcg
    73801 ggttttaaag tgatcatgct ggaagtggaa aaaccgacag tgattcgttg taccgtggcg
    73861 tttgcccagg ccgtgttcga tggcgaaatg acggtcgaag gcgtcactgc tcgcctggca
    73921 accagctctg cggaagcgat gaaacttacc gaacgcggat tcatccctgt gatggtagat
    73981 cccgcctgtt cattgcttga tgaactgaaa ccgctttgcg tggtggacgc tattctggcg
    74041 aaacagaatt tgggaacacg ggcagatatg gcaccagtaa caatcgcgct tgggccgggc
    74101 tttgctgcag ggaaggattg tcatgcggta attgaaacaa atcgcgggca ctggctcggt
    74161 caggtgattt actgtggttg tgcgcaggag aataccggtg ttcctggcaa tattatgggg
    74221 cataccaccc gacgggtgat ccgtgctcct gctgcaggca ttatgcgatc caacgtgaaa
    74281 ttaggcgatc tggtgaaaga gggcgatgtg attgcctgga ttggtgagca tgaaattaaa
    74341 gcaccgttga cggggatggt gcgtggcttg ttgaacgacg gcctggcagt ggttggtggt
    74401 tttaaaattg gtgatatcga tcctcgtggt gaaaccgctg atttcaccag cgtttctgat
    74461 aaagcccggg cgattggcgg cggcgtactt gaggcgttaa tgatgttgat gcatcagggc
    74521 gtgaaagcga caaaagaagt gctggaagtg gcttaaatga aaatggccgg atggtattac
    74581 ccacccggcc ttaaaattac tgacaaatca ccgttccggt tttgccttca atcccttctt
    74641 tcgctttgct caatacggta atcagtgctt cacgacctgc gcgagagcga gcaaacgagg
    74701 cggcagcttc cactttaggc agcatagaac ctttcgcaaa atgaccttct tcgataaagc
    74761 gttcagcatc acttaatgac aggcgatcga gccactgttc attctcttta ccgaagttaa
    74821 tggccacttt ttctaccgca gtcagaatga tcagcatatc ggcatcaatc atttctgcta
    74881 aacgggcgct ggcccagtct ttatcgataa ccgcgctggc accacgcaga tggttacctt
    74941 cacgaataac aggaatacca ccaccgccaa cggtgatcac cacctggcct gcttctacca
    75001 gcgcttttac cgtctctttt tcaatgatat caaccggttt tggcgaggca acaacacggc
    75061 gatagccgcg accagcatct tctttcagag tgtaaccctg ttttgtcagt tgctctgctt
    75121 cttgctcggt aaagaacgaa ccgatcggct tggtcgggtt gaggaatgcc ggatcgttag
    75181 cgtcgacttc gacctgagta accagcgtta ctacaggttt attaatgccc cgagaaagca
    75241 gttcttctct taaggcgttt tgtagatcgt aaccaatata accctggctt aaggcaacac
    75301 agacagacat cggcagcatt ggcgaatgcg cttcagtttt tgcggcagct tcaaaggcct
    75361 ggttaatcat gcccacctgc ggcccgttac catgagtgac gataacttca tgtccctggg
    75421 caattaaatc aacaattgcc tgagaagtaa tttttaccgc tttcatttgt ccggccaggt
    75481 catcgcccag cgcattcccg cccagggcga gaacaatttt cttactcatt tttcctcact
    75541 catcattctt gattgtgtat agatccctgg cagtggttag agcacgggag ggacaaacgg
    75601 tttgcgtcgc aggaaacgac ctcgcccggc tttgcctgta aaggtgccgt cacagaaaat
    75661 cgtttcacca cgggataagg ttctgacaat cgcgccctga caggtaaaac cctcccatgg
    75721 cgagtagtcg gcgttgtcgt ggagatggcg atgctgaatt tgttggctct gacgtgggtc
    75781 gataatcacc acgtcgccat cggaaccggg cgctaataat cctttttgcg gccacaggcc
    75841 aaacagcctg gcgggcattg cgctggttaa ttcaacaaag cgttccggtg ttatacgtcc
    75901 cgtcatcacg ccactggaaa acagtaactg catgcggttt tccacaccgg gtaagccatt
    75961 tgggcagcga ctgaaatcgc ctttagaaat ttgcaggcgt tgagccatcg agaaggtgca
    76021 gtgatcggtc gccaccacgt caatcgcacc atcgctgatg ccacaccaca gtttgtcctg
    76081 ctcgcgtacg ttacgcagcg gtgggctaag aatgaacttc atgccgtctt ctgtatcgta
    76141 actgcgttcg tccaacagga gatattgtgg gcaggtttca acccagactg gctggtgatt
    76201 cgcacgggca agacgcagat aatccagacc taagccgtta gacaggtgca cgatatagag
    76261 cggggcgtta ccggcaattt gtgccaggtt aatcatgcgg gcgatggctt ccgcttcgca
    76321 ttccagaggg cgactcaagg catgatagcg cggcgcggtt aaccctgcgg cgataaattc
    76381 cgcccgcttg ctggcgatag ccgcatcatt ttccgggtgc acggtggtca gcgcgccgga
    76441 ttcatgcaga cggcgtaatg cctgcaaaac ctcgtcatcg ttgagtttgt attgataggt
    76501 taaatagagt ttaaaactgc tcagcccttc ctcgaccatc atcgggattt cgtcgaggat
    76561 ggcgtgatta atgtgctgga tcacaccgtg aaagctgtaa tcgatgaccg ctttatgggc
    76621 ggcataacca cgataaacct ccagttgatg gcgtaaccga cagccgtttg ggccaaatcc
    76681 catatggtca ataatggttg ttgtaccgcc acacgcagct gcgcgggtac cggtaaaaaa
    76741 atcatcacaa ctgcgcgcga tgccgacatc aatattgaaa tgcgtatgga catccacgcc
    76801 gccagggaaa acgtaacagc cagtggcatc aatttcttca tacgggagct gcggcgaaat
    76861 attgttgccc aactggcgaa caatcccgct ttcaatcagc aaatcctgtt tggcttgtcc
    76921 atctgcgtta acgacagtgc cgtttttgat caatacgcgc atagcaaact ccaaaaaccc
    76981 tccggcgaac cggaggggaa taagtgaaga gtaattattc ggttgccagc cagcttaacg
    77041 ggattgcagc gtacatcgcg gcacaggtca ccaggtgaga tttccaggtt ttttcgttag
    77101 gtgcatgcgc ttcaggttct ttacccgggc caaagccgat caccggaatg ccgtgacggc
    77161 ccatgataga tacgccgtta gttgagaagg tccacttatc aacaaccggc gctttgccaa
    77221 acagcccttc gtaggcattc accagtgctt taacggtgaa gtgatcttct tccactttcc
    77281 aggtcgggaa gtagcattcg gttgggtaaa ccaggccagt ccaggacgga cggtcgtagt
    77341 tgtacataga aacaaccgcg ttagctttct gtactgcagg cagggcgcgg atttcgtcca
    77401 gcgcgccttc ccaggtttcg ccccaggtca gacggcggtc aatagagacg gcgcagctat
    77461 ctgctacagc gcaacggctt ggggatgtga agaagatttc agaaacggtg agggtgcctt
    77521 tgccgaggaa ttcgtcataa cccagacgtt gggagagttc ttgtaattcg ccaagaatcg
    77581 gacccatttt gaaaatggcg ttgtcaccgc gttctggcgc agaaccgtgg cagctaacac
    77641 cctgaacatc aatacgaatt tccatacgac cgcgctgacc acggtatacc tggcagtcgg
    77701 ttggttcggt actgaccaca aattccgggc gaatgccgga ttgttcaata atgtactgcc
    77761 agcacagacc gtcgcagtct tcttcctgca cagtaccggt aaccagcagg gtatattcat
    77821 cttccagacc gaggtcttta atgattttac cggcataaac catagatgcc atgccgcctt
    77881 cctggtcgga agtaccgcga ccaccgatca gctcatcagt ttccatgcct tcgtacggat
    77941 cgaagtccca gtttttgatg ttgccaatgc cgacggtatc gatatgagcg tccattgcca
    78001 ccagacgcgg gccgtggccg atataaccga gaacgttacc catcgggtcg atttcaactt
    78061 tatcgaagcc gactttttcc atctcttctt taatacgatg tactacccgt ttctcgtcgc
    78121 agctttcact gggaatagca accatgtcgc gcaggaagcg agtcatatcc gcctggtaat
    78181 cttttgcttt ttcaagaatc agtttgaatg gaatattctt agccattatt tttctccaga
    78241 ttttttactc cccgttttgc agggagcagt tccaataggg tgattaaggt gctacagcgt
    78301 gtttgccttc ccagacaact tcgcggtagt gcttcacgtc ggtgtcgcct tcagtgctga
    78361 taaccagcac tacggcatct ttgttcagcg ccagtttttc catcaggctt tgacgttgcg
    78421 ggtgataatg aaccgctgcg agaacgccca aaccgacagc gccggattca ccggagatga
    78481 tgcgcgggtc gttgccgtac ggattaccca gcacgcgcat acctaatgcg gcaacgctgt
    78541 cctggcagga gatgaattgg gtggcgcagt tacgtaggat ttcccagccc agcgggttag
    78601 gttcgccaca ggccaggcct gccatgatgg tggccatatc accgccaacg ttgacgatgt
    78661 cgcctttgac gccggagcga taaatacagt cagctttgtc aggttcaaca ataatgctgt
    78721 gcagattttg cgggctatag acgtcgacca gataacccag cacaccaccg gccattgctc
    78781 cgacaccggc ttgcagcaga acgtgcgtcg gggttacgcc catttcacgc atttgctcga
    78841 cggcttcatc tgccagggtt gcgtagcctt gcatgatcca ggttgggatt ttggtgtaac
    78901 cttcccatgc cgtgtcctgt accacttccc agccgtgctg ctgcgcgtgt tgcatggtca
    78961 ggcgaacggt atcgtcatag ttcatatctg tgacgatgca ctcggcaccg aggttcagaa
    79021 tggcgtcaac gcgttcctga gcagaacctt tcggcatgta aatcaccgca ttctgtccga
    79081 gttgctgcgc tgcccacgcc acaccgcgcc cgtggttgcc gtcggtggtg gtcgcgaaag
    79141 tcattttttc gccgatggca tttttcaggt gctcaaatga cagcgtttcg atatcaagat
    79201 gatatttttc acacaataat tgagcgatag cgtacgcacc gccgagcatt ttgaaggcgt
    79261 tcagaccgaa tcgttttgat tcgtctttga caagaatttt cttcacccca aaaaggtttg
    79321 cgagatcgtc cagcgcacaa agcggtgttg ggcgataacc agctattttc tggtggaact
    79381 ggcgcgccag tttggcctgg ctttgcgaaa agagcggtga tgtttcgccg ttgaaaaatt
    79441 tgttatcggc gatatcaatc ttcaatgaga aaacggacat atcctatcct tttggcctct
    79501 ggaaatagag ggccgaccag ctgatcggcc cgaggggtta tttcacgcgt tcttgcgctt
    79561 ctttcagcag ttgctcgagc agtgcacctg gtttggcgta tttacgggac aggatcatcg
    79621 cggcgatgat ataaggcttc cagctggctt ctttgtaggt agcgatacgg tatttttcga
    79681 atacgccttc agtcacttcc ccttctttgc aggatacgcc gctgatatca gctggcaggc
    79741 agtgcatgta cagggcttcg ccatcacggg tcagttccat catctcttca gtacaatgcc
    79801 agtctttgtg ttgcgcgttc tgtgccagac actgtttttc cagtgctttt aagccttcgt
    79861 gatcgttagc gcgcagcaat tcggtacgct gttccatcac tttgtaaggt gcccatgact
    79921 tcggataaac gatgtctgca tctttgaacg cttcttccat gctggtgacc tgacggaagc
    79981 taccgccaga tgctttagca ttgtttttcg cgacttctac tacatccggg atcaggtcgt
    80041 agccttccgg atgggccagg gtgacatcca taccgaagcg agtcatcaga ccgatgatgc
    80101 cttgtggtac agagagcggt ttgccatagc ttggagagta ggcccaggtc atggcgattt
    80161 ttttaccttt caggttttcc agtgaaccaa agtgttcacg taaccatgcc aggtcagcca
    80221 ttgactgagt cgggtggtca atatcgcatt gcaggttcac taaagccgga cgctgtggca
    80281 gtacaccctg cttgtaaccg tcatcaagtg cagcgccaac ttcacgcata taggcgttgc
    80341 ctgcgcccag atacatatcg tcgcgaatac caatagcgtc ggcgcagaag gagatcatat
    80401 tggcggtttc acgcacggtt tcgccgtgag cgatttgtga tttgccttca tcgagatctt
    80461 gctgtgcaag gccgagcagg ttaagcgcgg aagcataaga gaagcgggta cgagtggagt
    80521 tgtcgcggaa tacggaaata cctaatccac tattaaagac tttggttgag atgttttcag
    80581 cacgcagtgc ttttaatgct gcggcaacgt ccagtacttg tttcagttca tctggagtct
    80641 gttcccacgt taacaaaaaa tctttctcgt gaaggtgaga ggtcagcgaa ttgatatcct
    80701 taatcagctc attaacagtt ttcatcataa agtcccggta gtggaatgac aggcaaagtg
    80761 gtttatttca ctgtgacttg ccaaagtgat tggtgttttc acagctatac ctgggactaa
    80821 caataagtgt gccagaatta tccacggggc tgcaaaagac taatttgacg gataatgtgt
    80881 ggggttgatc acaaattgat gccagcttcc ggttgtttgt aaaatacgaa aacgtttggc
    80941 tttttttatc gcgttattta tttgctgcta agtatcagaa ataagataaa gggacaggct
    81001 atccctcgtc agtttgataa actcaccgcc ataattctca tattgatata gaaatgtgat
    81061 atctcttcca taatttcatt agaaagtgat aaaaatctta tgtttttatc aaagccaatt
    81121 tcgcctgagt taatatgatc atgatctgtg aaccatcaac gtcttcaatg atcgactttt
    81181 aagtaagtct ggaaggtaaa gctatggagc ttgctactac gcagtcagta ttgatgcaaa
    81241 ttcaaccgac aattcagcgt tttgccagaa tgcttgccag cgttttgcag cttgaggttg
    81301 agatcgttga tgaaaacttg tatcgcgtcg ccggaacggg cgcgtatggg aagtttcttg
    81361 gtcgccagtt gagcggcaac tcacgcctgc tctgccacgt cctggaaacg aaaactgaaa
    81421 aagttgtgac acagtctcgc ttcgatcccc tttgcgaagg ttgcgatagt aaagaaaatt
    81481 gccgcgaaaa agcatttctg ggtacgcctg tcattttaca ggatcgttgt gttggggtga
    81541 taagtttgat tgccgttacc cacgagcaac aagagcatat cagtgataat ttacgcgaat
    81601 tttctgatta tgttcgccat atatccacca tttttgtttc gaaacttctg gaggatcagg
    81661 ggccaggaga taacatcagt aaaatattcg cgaccatgat cgataatatg gatcagggcg
    81721 tattagttgt tgatgatgaa agtcgggttc agtttgttaa tcagactgcc ttaaaaacac
    81781 ttggtgttgt gcaaaataat attattggga aacctatccg tttcagacca ttaacatttg
    81841 agagtaattt tacccatgga catatgcagc atattgtttc gtgggacgat aaaagtgaat
    81901 taatcattgg tcaattgcat aacattcagg gccgacaatt atttttaatg gcgtttcacc
    81961 aatcgcatac cagtttttct gtagcaaatg cacctgatga accgcatatt gaacaattgg
    82021 ttggcgagtg ccgtgttatg cggcaattaa aacgactcat tagccgtatt gcacccagcc
    82081 catccagcgt tatggtggtt ggtgaaagcg gcacgggtaa agaagttgtc gcccgtgcaa
    82141 tccataagtt gagcggaaga cggaataaac cctttattgc tatcaactgt gccgcgattc
    82201 cggagcagct tctggagagc gaactgttcg gttatgttaa aggcgcattt actggcgctt
    82261 ctgccaacgg taaaacaggg ttgattcagg cggcgaatac gggcacgctg tttctcgatg
    82321 aaataggtga tatgccatta atgttgcagg ctaaattact gcgcgctatt gaggcacgtg
    82381 aaattctgcc gattggtgcc agtagcccaa tacaagtcga cattcgcatc atttctgcaa
    82441 ctaaccagaa tttggcccag ttcattgccg aaggtaaatt ccgcgaagat ctcttctacc
    82501 gacttaatgt tatcccgata actctgccac cgctgcgtga acgtcaggaa gatattgaac
    82561 tactggtgca ttacttttta catctgcata cccgtcgtct gggatcggtt tatcctggca
    82621 ttgctcccga tgtcgtcgaa atattgcgta agcatcgttg gcccggaaac ctgcgcgagt
    82681 taagcaattt gatggaatat ctggttaacg ttgttccttc aggtgaagtt atcgacagca
    82741 cgctattgcc gccaaatctg ctgaataatg gcacaacgga gcaaagtgat gtaacagagg
    82801 ttactgaggc acacctgtca ctcgatgatg cgggcggcac ggcgctggag gagatggaaa
    82861 agcaaatgat ccgcgaggcg ctttcacgtc ataacagcaa gaagcaagtt gctgatgaac
    82921 tgggcatcgg cattgctacg ctctatcgca agattaagaa atatgagttg ttaaacacat
    82981 aacatcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt tttctcgcaa
    83041 tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt tcccgccagt
    83101 ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag cattgccgtg
    83161 gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgcgccgga tttcgcataa
    83221 gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt gcggatctct
    83281 tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc gtcgaccagc
    83341 accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag cagcccttgt
    83401 tcgcggagta attccgagag cggcgtgcct ggtacggcat gaagctgaaa aggcatcccg
    83461 ttaatggtgc attcgatggt aattgtttcg ctgtgattca ttgcaatttt ccccccgccg
    83521 cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc agacgaaact
    83581 ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat tcgctgatag
    83641 cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca tgttggcagc
    83701 gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa ttgccgttat
    83761 ccagtcggca atgtgcggca cagccaatcg ttgaaatatc cattgcgtcg cgcatggcat
    83821 atttaaaatg cgcgctgccc acgtgttctt tcggctgggg cggaaaatga aaggcgacaa
    83881 ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca ttaatcggga
    83941 cgaaacgaac accgcgtggg gagtggatct ccagtttcgc gtcataaatg agcgttggcg
    84001 tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta gcgacgttac
    84061 ggatctgcgg tccagcaatg gacgaggccg cagcacataa cgccgggaga tgacgttgag
    84121 ttatagaatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt agcgagccat
    84181 cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca atatggcgat
    84241 aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg agcagtttgg
    84301 cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg cgatggtaag
    84361 aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa ctcttcatat
    84421 aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat cttcaccgcg
    84481 ttacgaatag cagcggcaac aggaattatt ggtggctcac ccagtgactt atgtccgtat
    84541 gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag atccggcatg
    84601 gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt cgcatcgatg
    84661 atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg tacctgacct
    84721 tctgccagta gcggattaag aatatggccc gaatcatgaa cgttgaggat gcggttgatg
    84781 gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc gaacgccggt
    84841 gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg ttcagggtgg
    84901 tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg tcgttcaacc
    84961 agcacgatat ggccttttat cagggtcaga ttcatcgctg actgatgtag catgactgcg
    85021 gcgtgagcga tgattttctc ttttaataac agtgccgcac tgcgcagcgc aggcgcggca
    85081 acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc ggtatcttgt
    85141 gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac catttgcgag
    85201 aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt gatggttcca
    85261 tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca ggtgttagag
    85321 gtgtagctaa aacaggcgac gccaacgccg cggcgtaaat tgccttgctg gttctggcac
    85381 tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca ctccggcaac
    85441 cctgcgctgt aaatacgttt gcccgtgagc ggattagcat ctccttcgcg tgaggcgttg
    85501 cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc aagcatagac
    85561 tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc cgaggggagg
    85621 ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag gtaagcgact
    85681 ttatttcccc ctgcagaagc gatggagtgc ccgtgagata cataagcgcc ggtgttagac
    85741 agaacatcca gactataacc tttcaatgtt ccgtcgcggt tcacgcccat ttgcccgtca
    85801 atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct aagggaaact
    85861 ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc ttccagtaca
    85921 tcctgtttat taccaaaacc gccaccgaca aatggtttga tgactcgtac gcatgaccag
    85981 ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg ggtgctggaa
    86041 acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct ttccatgtga
    86101 caatgttgaa taacgggggt ctgatagtgc ccctgtacct ggtagtcggc ggcatcgatt
    86161 gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc gccgttatgg
    86221 attggtgcag cgtcctctgc cagcgccgct tctggcgtgg tgataacggg taattcttcc
    86281 cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg ggccacgacg
    86341 atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg atcggcggta
    86401 tcgcgcttgt tttcgtcaag tgtccatgca tgcccagctg tagcgaatgg gatatcaggc
    86461 acatcttccc aggtaaaaat cgccagtacg cctggtaaac ttctggcttg ttcatcatta
    86521 atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcata acacatgccc
    86581 gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat ggcatcaacg
    86641 cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat cccctcgatt
    86701 tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta cgccagaaac
    86761 ggcgataagc gttaagggag gaaggaagtg tgatgatgtt aaaaaaacac agatatgata
    86821 cgaaacagga ccctgaatag gggtagtgat acgtatcaat atgagtggcg ataaaatcaa
    86881 tgtgataaat taggattatt acgcatcagg atgggaaata gggtaaaaaa gtcaggaact
    86941 tcgatacctc tacagtgaat attttgctga tgatacttcc ctttgccaaa tgaaattaac
    87001 ctctttctct attacatcgt ttttccagaa gcaagagatt gggtatcaag aggctggctg
    87061 ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg acgcctgaat
    87121 aaaaaatctc tgggtatcgt gatgttgtta tcggttggac tgcttttggc gggctgttcg
    87181 ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt gaaacggggg
    87241 gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct ggcgcgactg
    87301 aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact gggtggggcg
    87361 aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgaccaaaac cgcatcggtt
    87421 acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg ttgttggtta
    87481 tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg caataaaggg
    87541 attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg aaaggtggtg
    87601 tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca cagtgaagat
    87661 tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca aagcgtgaag
    87721 gctgggcaaa aaatcgccac catggggagc acggatgcgg catctgttcg cctgcatttc
    87781 cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc tcagggcagc
    87841 aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt gctaagaaga
    87901 gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg gtagaacgag
    87961 agcttcccaa gctctatacg agggttcgat tcccttcgcc cgctccaatt tatcttcacc
    88021 tcatcaatct gccatttttg ctgtatctga ttttctatat tatcttaatt caatcgcttc
    88081 agacttttcg caatctctgt aacactcccc catttttttg taaataaaaa gcgtttacgc
    88141 aatgcaatcc ttttgtaaca acccaggtaa aataccttat tttcaagttg ttaaataagt
    88201 ggttatctac gcttttaaca aacgattgat ggaatatttt atgaaaggga aatctgcatt
    88261 aactcttctg ctcgctggta tattttcgtg cggcacatgc caggcgacag gggcagaagt
    88321 gacgagtgaa tctgtattta acattcttaa ttccacgggg gcagcaacag ataaaagtta
    88381 tttatcgtta aatccagata agtatcctaa ttatcgtctt ctgattcact cagccaaatt
    88441 acaaaatgaa ataaagagcc actataccaa agacgaaatt caaggattgc taacattaac
    88501 agaaaacaca cgaaagttaa cgttgacgga gaaaccctgg ggaacgttta ttcttgcttc
    88561 tactttcgaa gatgacaaaa cagcggcaga aacccactat gatgctgtct ggctgaggga
    88621 tagtttatgg ggctatatgg cattagtatc agaccaggga aatagtgtag ccgcaaaaaa
    88681 ggttctgctc accctgtggg attatatgtc tacgcccgac cagattaagc ggatgcagga
    88741 catcattagc aacccaaaac gactggatgg tattcccggg caaatgaatg ctgtgcatat
    88801 ttgacagcaa ctcccccgtg atggcagatg tgcaggaaga aggcaagccg cagctgtgga
    88861 accataaaca aaatgatgcg ttaggtcttt atcttgatct ccttatacaa gcaatcaata
    88921 ctggtactat taacgctgaa gattggcaaa aaggtgaccg actaaaatcg gttgcgctgc
    88981 tgattgctta tctggataaa gccaatttct atgttatgga agattccgga gcgtgggagg
    89041 aagatgcccg attaaacaca tcttccgttg cactggtgac ctctggactg gaacgtttgt
    89101 caaacctact ctcgaagaaa gactctgttt ttgtctctga tttactacgc gaagctaaag
    89161 tcaatgaact ggatgaaact ctttcaacaa cgaggttgaa ccacctcata gataaaggtt
    89221 atgaaagaat aacgttacag cttgatttag ggggagaatc cccaggttat ctggaaaaag
    89281 ataaacatta cagagaagcc gatgcggctt tacttaatgt tatttatcct gcaaatcttt
    89341 caaagataaa caccagaaga aaagagcaag ttctaaaaat agttaaaaaa ctagcaggtc
    89401 cgtatggtat aaaaagatac gagaaggata attatcagtc ggcaaatttt tggttcaatg
    89461 atattaaaac cgataccgat caaaattcgc atgcaaaaag agaaaagagc ttcattccat
    89521 caacagaagc agaatggttt tttgactcat ggtatgcaaa atcagcagca attgtttata
    89581 aagaatcaag aaaagaagag tatttgaatg actcagtaca atttatgaat agatcattgg
    89641 cccaaataac aggcgagaat atgattggtg caaatggcag gagcgtacca gaaatggcac
    89701 ttcccgaaag ctataactat atccacaagt cgggaacttt gcatgaagcg ccaagcccta
    89761 tcattccgct aaattggtcg aaagcttcaa tgactctcat gttgaaagag atgtccaacc
    89821 ttatcaatga tgagggaatt aaataatttt cataatgagg cggtcctgga ttaaaaggac
    89881 cgctcaaaga gaaatcgaaa ggggaggtaa aagtgatcaa taaattactg ttagcttacc
    89941 tgataggtct ggttgtgaca tcgactttaa tatttatctt ctcggaagaa aaagtaacat
    90001 atcggttgtt tgctgcaata attacaggtt taacctggcc gcttagtctc attccttcca
    90061 ttataagtct catgattcgt aaatccgact aacaagaaaa ttatatgatc gattaagaaa
    90121 aataga
//
LOCUS       DABSYT020000010        84041 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.010, whole genome shotgun sequence.
ACCESSION   DABSYT020000010 DABSYT020000000
VERSION     DABSYT020000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 84041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.010"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            391..4374
                     /gene="ehaA"
                     /locus_tag="ID725_RS04785"
                     /old_locus_tag="pgaptmp_000957"
     CDS             391..4374
                     /gene="ehaA"
                     /locus_tag="ID725_RS04785"
                     /old_locus_tag="pgaptmp_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGV7849612.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4516..5604
                     /gene="pdeL"
                     /locus_tag="ID725_RS04790"
                     /old_locus_tag="pgaptmp_000958"
     CDS             4516..5604
                     /gene="pdeL"
                     /locus_tag="ID725_RS04790"
                     /old_locus_tag="pgaptmp_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGV7849613.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5646..6577)
                     /gene="yahB"
                     /locus_tag="ID725_RS04795"
                     /old_locus_tag="pgaptmp_000959"
                     /pseudo
     CDS             complement(5646..6577)
                     /gene="yahB"
                     /locus_tag="ID725_RS04795"
                     /old_locus_tag="pgaptmp_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6669..7176)
                     /gene="yahC"
                     /locus_tag="ID725_RS04800"
                     /old_locus_tag="pgaptmp_000960"
                     /pseudo
     CDS             complement(6669..7176)
                     /gene="yahC"
                     /locus_tag="ID725_RS04800"
                     /old_locus_tag="pgaptmp_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7434..8039
                     /gene="yahD"
                     /locus_tag="ID725_RS04805"
                     /old_locus_tag="pgaptmp_000961"
     CDS             7434..8039
                     /gene="yahD"
                     /locus_tag="ID725_RS04805"
                     /old_locus_tag="pgaptmp_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGV7849614.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            8079..8942
                     /gene="yahE"
                     /locus_tag="ID725_RS04810"
                     /old_locus_tag="pgaptmp_000962"
     CDS             8079..8942
                     /gene="yahE"
                     /locus_tag="ID725_RS04810"
                     /old_locus_tag="pgaptmp_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGV7849615.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8932..10479
                     /gene="fdrA"
                     /locus_tag="ID725_RS04815"
                     /old_locus_tag="pgaptmp_000963"
     CDS             8932..10479
                     /gene="fdrA"
                     /locus_tag="ID725_RS04815"
                     /old_locus_tag="pgaptmp_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGV7849616.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10479..11897
                     /gene="yahG"
                     /locus_tag="ID725_RS04820"
                     /old_locus_tag="pgaptmp_000964"
     CDS             10479..11897
                     /gene="yahG"
                     /locus_tag="ID725_RS04820"
                     /old_locus_tag="pgaptmp_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGV7849617.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            11923..12243
                     /gene="yahH"
                     /locus_tag="ID725_RS04825"
                     /old_locus_tag="pgaptmp_000965"
     CDS             11923..12243
                     /gene="yahH"
                     /locus_tag="ID725_RS04825"
                     /old_locus_tag="pgaptmp_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
                     /protein_id="HGV7849618.1"
                     /translation="MNALSGLRMAQESVGLISVAHQAFVTIAGCGVNALSGLQVAQEF
                     VGLISVAHQAFITIAGCGVNALSGLRMAQESVGLISVAHQAFVTIAGCGVNALSGLRM
                     AREL"
     gene            12270..12362
                     /gene="yahH"
                     /locus_tag="ID725_RS04830"
                     /old_locus_tag="pgaptmp_000966"
                     /pseudo
     CDS             12270..12362
                     /gene="yahH"
                     /locus_tag="ID725_RS04830"
                     /old_locus_tag="pgaptmp_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            12412..13362
                     /gene="yahI"
                     /locus_tag="ID725_RS04835"
                     /old_locus_tag="pgaptmp_000967"
     CDS             12412..13362
                     /gene="yahI"
                     /locus_tag="ID725_RS04835"
                     /old_locus_tag="pgaptmp_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGV7849619.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            13372..14754
                     /gene="yahJ"
                     /locus_tag="ID725_RS04840"
                     /old_locus_tag="pgaptmp_000968"
     CDS             13372..14754
                     /gene="yahJ"
                     /locus_tag="ID725_RS04840"
                     /old_locus_tag="pgaptmp_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGV7849620.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            15053..15463
                     /locus_tag="ID725_RS04845"
                     /old_locus_tag="pgaptmp_000969"
     CDS             15053..15463
                     /locus_tag="ID725_RS04845"
                     /old_locus_tag="pgaptmp_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGV7849621.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            15717..16700
                     /locus_tag="ID725_RS04850"
                     /old_locus_tag="pgaptmp_000970"
     CDS             15717..16700
                     /locus_tag="ID725_RS04850"
                     /old_locus_tag="pgaptmp_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HGV7849622.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            16749..18233
                     /locus_tag="ID725_RS04855"
                     /old_locus_tag="pgaptmp_000971"
     CDS             16749..18233
                     /locus_tag="ID725_RS04855"
                     /old_locus_tag="pgaptmp_000971"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGV7849623.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            18226..19197
                     /locus_tag="ID725_RS04860"
                     /old_locus_tag="pgaptmp_000972"
     CDS             18226..19197
                     /locus_tag="ID725_RS04860"
                     /old_locus_tag="pgaptmp_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7849624.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            19194..20150
                     /locus_tag="ID725_RS04865"
                     /old_locus_tag="pgaptmp_000973"
     CDS             19194..20150
                     /locus_tag="ID725_RS04865"
                     /old_locus_tag="pgaptmp_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7849625.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            20237..21286
                     /gene="yahK"
                     /locus_tag="ID725_RS04870"
                     /old_locus_tag="pgaptmp_000974"
     CDS             20237..21286
                     /gene="yahK"
                     /locus_tag="ID725_RS04870"
                     /old_locus_tag="pgaptmp_000974"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGV7849626.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            21529..22344
                     /gene="yahL"
                     /locus_tag="ID725_RS04875"
                     /old_locus_tag="pgaptmp_000975"
     CDS             21529..22344
                     /gene="yahL"
                     /locus_tag="ID725_RS04875"
                     /old_locus_tag="pgaptmp_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGV7849627.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            22796..23005
                     /locus_tag="ID725_RS04880"
                     /old_locus_tag="pgaptmp_000976"
     CDS             22796..23005
                     /locus_tag="ID725_RS04880"
                     /old_locus_tag="pgaptmp_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849628.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(23022..23693)
                     /gene="yahN"
                     /locus_tag="ID725_RS04885"
                     /old_locus_tag="pgaptmp_000977"
     CDS             complement(23022..23693)
                     /gene="yahN"
                     /locus_tag="ID725_RS04885"
                     /old_locus_tag="pgaptmp_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGV7849629.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            23840..24115
                     /gene="yahO"
                     /locus_tag="ID725_RS04890"
                     /old_locus_tag="pgaptmp_000978"
     CDS             23840..24115
                     /gene="yahO"
                     /locus_tag="ID725_RS04890"
                     /old_locus_tag="pgaptmp_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGV7849630.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(24213..25799)
                     /gene="prpR"
                     /locus_tag="ID725_RS04895"
                     /old_locus_tag="pgaptmp_000979"
     CDS             complement(24213..25799)
                     /gene="prpR"
                     /locus_tag="ID725_RS04895"
                     /old_locus_tag="pgaptmp_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGV7849631.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            26038..26928
                     /gene="prpB"
                     /locus_tag="ID725_RS04900"
                     /old_locus_tag="pgaptmp_000980"
     CDS             26038..26928
                     /gene="prpB"
                     /locus_tag="ID725_RS04900"
                     /old_locus_tag="pgaptmp_000980"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGV7849632.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            27084..28253
                     /gene="prpC"
                     /locus_tag="ID725_RS04905"
                     /old_locus_tag="pgaptmp_000981"
     CDS             27084..28253
                     /gene="prpC"
                     /locus_tag="ID725_RS04905"
                     /old_locus_tag="pgaptmp_000981"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGV7849633.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            28287..29738
                     /gene="prpD"
                     /locus_tag="ID725_RS04910"
                     /old_locus_tag="pgaptmp_000982"
     CDS             28287..29738
                     /gene="prpD"
                     /locus_tag="ID725_RS04910"
                     /old_locus_tag="pgaptmp_000982"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGV7849634.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            29778..31664
                     /gene="prpE"
                     /locus_tag="ID725_RS04915"
                     /old_locus_tag="pgaptmp_000983"
     CDS             29778..31664
                     /gene="prpE"
                     /locus_tag="ID725_RS04915"
                     /old_locus_tag="pgaptmp_000983"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGV7849635.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
     gene            32558..33817
                     /gene="codB"
                     /locus_tag="ID725_RS04920"
                     /old_locus_tag="pgaptmp_000984"
     CDS             32558..33817
                     /gene="codB"
                     /locus_tag="ID725_RS04920"
                     /old_locus_tag="pgaptmp_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGV7849636.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            33807..35090
                     /gene="codA"
                     /locus_tag="ID725_RS04925"
                     /old_locus_tag="pgaptmp_000985"
     CDS             33807..35090
                     /gene="codA"
                     /locus_tag="ID725_RS04925"
                     /old_locus_tag="pgaptmp_000985"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGV7849637.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(35223..36122)
                     /gene="cynR"
                     /locus_tag="ID725_RS04930"
                     /old_locus_tag="pgaptmp_000986"
     CDS             complement(35223..36122)
                     /gene="cynR"
                     /locus_tag="ID725_RS04930"
                     /old_locus_tag="pgaptmp_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGV7849638.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            36232..36891
                     /gene="cynT"
                     /locus_tag="ID725_RS04935"
                     /old_locus_tag="pgaptmp_000987"
     CDS             36232..36891
                     /gene="cynT"
                     /locus_tag="ID725_RS04935"
                     /old_locus_tag="pgaptmp_000987"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HGV7849639.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            36922..37392
                     /gene="cynS"
                     /locus_tag="ID725_RS04940"
                     /old_locus_tag="pgaptmp_000988"
     CDS             36922..37392
                     /gene="cynS"
                     /locus_tag="ID725_RS04940"
                     /old_locus_tag="pgaptmp_000988"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGV7849640.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            37398..38579
                     /gene="cynX"
                     /locus_tag="ID725_RS04945"
                     /old_locus_tag="pgaptmp_000989"
     CDS             37398..38579
                     /gene="cynX"
                     /locus_tag="ID725_RS04945"
                     /old_locus_tag="pgaptmp_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGV7849641.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSIRGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(38682..39293)
                     /gene="lacA"
                     /locus_tag="ID725_RS04950"
                     /old_locus_tag="pgaptmp_000990"
     CDS             complement(38682..39293)
                     /gene="lacA"
                     /locus_tag="ID725_RS04950"
                     /old_locus_tag="pgaptmp_000990"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HGV7849642.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(39359..40612)
                     /gene="lacY"
                     /locus_tag="ID725_RS04955"
                     /old_locus_tag="pgaptmp_000991"
     CDS             complement(39359..40612)
                     /gene="lacY"
                     /locus_tag="ID725_RS04955"
                     /old_locus_tag="pgaptmp_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGV7849643.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(40664..43738)
                     /gene="lacZ"
                     /locus_tag="ID725_RS04960"
                     /old_locus_tag="pgaptmp_000992"
     CDS             complement(40664..43738)
                     /gene="lacZ"
                     /locus_tag="ID725_RS04960"
                     /old_locus_tag="pgaptmp_000992"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGV7849644.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFHHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(43861..44943)
                     /gene="lacI"
                     /locus_tag="ID725_RS04965"
                     /old_locus_tag="pgaptmp_000993"
     CDS             complement(43861..44943)
                     /gene="lacI"
                     /locus_tag="ID725_RS04965"
                     /old_locus_tag="pgaptmp_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGV7849645.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(44980..45933)
                     /locus_tag="ID725_RS04970"
                     /old_locus_tag="pgaptmp_000994"
     CDS             complement(44980..45933)
                     /locus_tag="ID725_RS04970"
                     /old_locus_tag="pgaptmp_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HGV7849646.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(45953..46729)
                     /locus_tag="ID725_RS04975"
                     /old_locus_tag="pgaptmp_000995"
     CDS             complement(45953..46729)
                     /locus_tag="ID725_RS04975"
                     /old_locus_tag="pgaptmp_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGV7849647.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(46844..47677)
                     /gene="mhpR"
                     /locus_tag="ID725_RS04980"
                     /old_locus_tag="pgaptmp_000996"
     CDS             complement(46844..47677)
                     /gene="mhpR"
                     /locus_tag="ID725_RS04980"
                     /old_locus_tag="pgaptmp_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGV7849648.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            47868..49532
                     /gene="mhpA"
                     /locus_tag="ID725_RS04985"
                     /old_locus_tag="pgaptmp_000997"
     CDS             47868..49532
                     /gene="mhpA"
                     /locus_tag="ID725_RS04985"
                     /old_locus_tag="pgaptmp_000997"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGV7849649.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            49534..50478
                     /gene="mhpB"
                     /locus_tag="ID725_RS04990"
                     /old_locus_tag="pgaptmp_000998"
     CDS             49534..50478
                     /gene="mhpB"
                     /locus_tag="ID725_RS04990"
                     /old_locus_tag="pgaptmp_000998"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGV7849650.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            50496..51362
                     /gene="mhpC"
                     /locus_tag="ID725_RS04995"
                     /old_locus_tag="pgaptmp_000999"
     CDS             50496..51362
                     /gene="mhpC"
                     /locus_tag="ID725_RS04995"
                     /old_locus_tag="pgaptmp_000999"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGV7849651.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            51372..52181
                     /gene="mhpD"
                     /locus_tag="ID725_RS05000"
                     /old_locus_tag="pgaptmp_001000"
     CDS             51372..52181
                     /gene="mhpD"
                     /locus_tag="ID725_RS05000"
                     /old_locus_tag="pgaptmp_001000"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGV7849652.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            52178..53128
                     /gene="mhpF"
                     /locus_tag="ID725_RS05005"
                     /old_locus_tag="pgaptmp_001001"
     CDS             52178..53128
                     /gene="mhpF"
                     /locus_tag="ID725_RS05005"
                     /old_locus_tag="pgaptmp_001001"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGV7849653.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            53125..54138
                     /gene="mhpE"
                     /locus_tag="ID725_RS05010"
                     /old_locus_tag="pgaptmp_001002"
     CDS             53125..54138
                     /gene="mhpE"
                     /locus_tag="ID725_RS05010"
                     /old_locus_tag="pgaptmp_001002"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGV7849654.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHRYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            54314..55525
                     /gene="mhpT"
                     /locus_tag="ID725_RS05015"
                     /old_locus_tag="pgaptmp_001003"
     CDS             54314..55525
                     /gene="mhpT"
                     /locus_tag="ID725_RS05015"
                     /old_locus_tag="pgaptmp_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGV7849655.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            55627..56166
                     /gene="yaiL"
                     /locus_tag="ID725_RS05020"
                     /old_locus_tag="pgaptmp_001004"
     CDS             55627..56166
                     /gene="yaiL"
                     /locus_tag="ID725_RS05020"
                     /old_locus_tag="pgaptmp_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGV7849656.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(56291..57124)
                     /gene="fghA"
                     /locus_tag="ID725_RS05025"
                     /old_locus_tag="pgaptmp_001005"
     CDS             complement(56291..57124)
                     /gene="fghA"
                     /locus_tag="ID725_RS05025"
                     /old_locus_tag="pgaptmp_001005"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGV7849657.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYHPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(57217..58326)
                     /gene="frmA"
                     /locus_tag="ID725_RS05030"
                     /old_locus_tag="pgaptmp_001006"
     CDS             complement(57217..58326)
                     /gene="frmA"
                     /locus_tag="ID725_RS05030"
                     /old_locus_tag="pgaptmp_001006"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGV7849658.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(58361..58636)
                     /gene="frmR"
                     /locus_tag="ID725_RS05035"
                     /old_locus_tag="pgaptmp_001007"
     CDS             complement(58361..58636)
                     /gene="frmR"
                     /locus_tag="ID725_RS05035"
                     /old_locus_tag="pgaptmp_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGV7849659.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(58796..59842)
                     /gene="fbpC"
                     /locus_tag="ID725_RS05040"
                     /old_locus_tag="pgaptmp_001008"
     CDS             complement(58796..59842)
                     /gene="fbpC"
                     /locus_tag="ID725_RS05040"
                     /old_locus_tag="pgaptmp_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HGV7849660.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(59854..61932)
                     /locus_tag="ID725_RS05045"
                     /old_locus_tag="pgaptmp_001009"
     CDS             complement(59854..61932)
                     /locus_tag="ID725_RS05045"
                     /old_locus_tag="pgaptmp_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7849661.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(62001..63032)
                     /locus_tag="ID725_RS05050"
                     /old_locus_tag="pgaptmp_001010"
     CDS             complement(62001..63032)
                     /locus_tag="ID725_RS05050"
                     /old_locus_tag="pgaptmp_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGV7849662.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(63029..64345)
                     /gene="uhpC"
                     /locus_tag="ID725_RS05055"
                     /old_locus_tag="pgaptmp_001011"
     CDS             complement(63029..64345)
                     /gene="uhpC"
                     /locus_tag="ID725_RS05055"
                     /old_locus_tag="pgaptmp_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGV7849663.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(64418..65959)
                     /locus_tag="ID725_RS05060"
                     /old_locus_tag="pgaptmp_001012"
     CDS             complement(64418..65959)
                     /locus_tag="ID725_RS05060"
                     /old_locus_tag="pgaptmp_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGV7849664.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(65959..66588)
                     /locus_tag="ID725_RS05065"
                     /old_locus_tag="pgaptmp_001013"
     CDS             complement(65959..66588)
                     /locus_tag="ID725_RS05065"
                     /old_locus_tag="pgaptmp_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGV7849665.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPIFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            66891..67853
                     /gene="tauA"
                     /locus_tag="ID725_RS05070"
                     /old_locus_tag="pgaptmp_001014"
     CDS             66891..67853
                     /gene="tauA"
                     /locus_tag="ID725_RS05070"
                     /old_locus_tag="pgaptmp_001014"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGV7849666.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            67866..68633
                     /gene="tauB"
                     /locus_tag="ID725_RS05075"
                     /old_locus_tag="pgaptmp_001015"
     CDS             67866..68633
                     /gene="tauB"
                     /locus_tag="ID725_RS05075"
                     /old_locus_tag="pgaptmp_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGV7849667.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     IQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            68630..69457
                     /gene="tauC"
                     /locus_tag="ID725_RS05080"
                     /old_locus_tag="pgaptmp_001016"
     CDS             68630..69457
                     /gene="tauC"
                     /locus_tag="ID725_RS05080"
                     /old_locus_tag="pgaptmp_001016"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGV7849668.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            69454..70305
                     /gene="tauD"
                     /locus_tag="ID725_RS05085"
                     /old_locus_tag="pgaptmp_001017"
     CDS             69454..70305
                     /gene="tauD"
                     /locus_tag="ID725_RS05085"
                     /old_locus_tag="pgaptmp_001017"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGV7849669.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(70412..71386)
                     /gene="hemB"
                     /locus_tag="ID725_RS05090"
                     /old_locus_tag="pgaptmp_001018"
     CDS             complement(70412..71386)
                     /gene="hemB"
                     /locus_tag="ID725_RS05090"
                     /old_locus_tag="pgaptmp_001018"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGV7849670.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            71912..74840
                     /gene="ehaB"
                     /locus_tag="ID725_RS05095"
                     /old_locus_tag="pgaptmp_001019"
                     /pseudo
     CDS             71912..74840
                     /gene="ehaB"
                     /locus_tag="ID725_RS05095"
                     /old_locus_tag="pgaptmp_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
     gene            74928..75551
                     /gene="iprA"
                     /locus_tag="ID725_RS05100"
                     /old_locus_tag="pgaptmp_001020"
     CDS             74928..75551
                     /gene="iprA"
                     /locus_tag="ID725_RS05100"
                     /old_locus_tag="pgaptmp_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HGV7849671.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(75552..76709)
                     /gene="ampH"
                     /locus_tag="ID725_RS05105"
                     /old_locus_tag="pgaptmp_001021"
     CDS             complement(75552..76709)
                     /gene="ampH"
                     /locus_tag="ID725_RS05105"
                     /old_locus_tag="pgaptmp_001021"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGV7849672.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            77061..78281
                     /gene="sbmA"
                     /locus_tag="ID725_RS05110"
                     /old_locus_tag="pgaptmp_001022"
     CDS             77061..78281
                     /gene="sbmA"
                     /locus_tag="ID725_RS05110"
                     /old_locus_tag="pgaptmp_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGV7849673.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            78294..79388
                     /gene="yaiW"
                     /locus_tag="ID725_RS05115"
                     /old_locus_tag="pgaptmp_001023"
     CDS             78294..79388
                     /gene="yaiW"
                     /locus_tag="ID725_RS05115"
                     /old_locus_tag="pgaptmp_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGV7849674.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(79447..79755)
                     /gene="yaiY"
                     /locus_tag="ID725_RS05120"
                     /old_locus_tag="pgaptmp_001024"
     CDS             complement(79447..79755)
                     /gene="yaiY"
                     /locus_tag="ID725_RS05120"
                     /old_locus_tag="pgaptmp_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGV7849675.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            80015..80227
                     /gene="yaiZ"
                     /locus_tag="ID725_RS05125"
                     /old_locus_tag="pgaptmp_001025"
     CDS             80015..80227
                     /gene="yaiZ"
                     /locus_tag="ID725_RS05125"
                     /old_locus_tag="pgaptmp_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGV7849676.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(80251..81345)
                     /gene="ddlA"
                     /locus_tag="ID725_RS05130"
                     /old_locus_tag="pgaptmp_001026"
     CDS             complement(80251..81345)
                     /gene="ddlA"
                     /locus_tag="ID725_RS05130"
                     /old_locus_tag="pgaptmp_001026"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGV7849677.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            81423..81626
                     /locus_tag="ID725_RS05135"
                     /old_locus_tag="pgaptmp_001027"
     CDS             81423..81626
                     /locus_tag="ID725_RS05135"
                     /old_locus_tag="pgaptmp_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849678.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            81808..82068
                     /gene="iraP"
                     /locus_tag="ID725_RS05140"
                     /old_locus_tag="pgaptmp_001028"
     CDS             81808..82068
                     /gene="iraP"
                     /locus_tag="ID725_RS05140"
                     /old_locus_tag="pgaptmp_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGV7849679.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            82169..83584
                     /gene="phoA"
                     /locus_tag="ID725_RS05145"
                     /old_locus_tag="pgaptmp_001029"
     CDS             82169..83584
                     /gene="phoA"
                     /locus_tag="ID725_RS05145"
                     /old_locus_tag="pgaptmp_001029"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGV7849680.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            83703..84023
                     /gene="psiF"
                     /locus_tag="ID725_RS05150"
                     /old_locus_tag="pgaptmp_001030"
     CDS             83703..84023
                     /gene="psiF"
                     /locus_tag="ID725_RS05150"
                     /old_locus_tag="pgaptmp_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGV7849681.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
BASE COUNT        20400 a        21044 c        22610 g        19987 t
ORIGIN      
        1 tcctataggg ttatatattt attaataaga cgattcttat atttggatac ctaataagag
       61 ttaccacttt taccggctgt atattaaatg tatttctcct gcttttgttt atattttgag
      121 ttgatttgag ttcccggata catataatgc gcaggtatta gagactgggg gggaatgtaa
      181 tgataaagtt aagagtgtta acttaaaata agtttttagt agtaacttgc tattttatta
      241 ccctctgaac tacatactca gatatgtggt tgccaatgct atgcaaaatt tttgaatttt
      301 tatatgcgat gaacagtaac cggaatggca tttaatgcgt tgctgtttat gcaatcttgg
      361 ttttatttgg atgtattgct ggagattgtg atgaataaaa tatatcggct aaagtggaac
      421 aggtcccgta actgttggag cgtctgctcg gagctgggga gcagagtaaa aggaaaaaag
      481 tcccgggctg ttttaattag cgcgataagt ttatattcat ctctggtatt cgccgatgat
      541 gtcatcgtaa accaggataa aactattgat tttggcaaag agaaccagag catcgattac
      601 cgtattacgg tgacagacaa tgccaatctg gtaatcaatg cgacagatac ttcccgtccg
      661 cgtctgactc tcgcttctgg tggtgggttg gatattaccg gaggaaaggt aacaatcaat
      721 ggcccactta actttttgct gaaaggtacg gggttcctga atgtctccaa tgctggcagc
      781 gagttatatg ctgatgattt gtatgaatca aactcaggca tgagacacga tcgcggctat
      841 tttaatgtct ccaacggcgg caaaatccat gttaagggca ccagccgtct gacctatttg
      901 cagggaaatg tcagtggtga aggtagccag gtaaattccg aaaccttctt tatgggcgtt
      961 tacggcagtt acggtggtaa tcagtacctg tcagttaata acggcggtga agttaatgcc
     1021 aggaagcaaa ttagcctggg ctattatgat caagtctccg atacaacact tgctgtttcg
     1081 gaaggtggta aaatttctgc gcctactatt agtttaagca ccaactctga gttagcgtta
     1141 ggggcacagg aaggaagcgc agcgaaggca gcagggatta ttgatgccga aaaaattgag
     1201 tttgtgtggg caaagacatc cgagaagaaa atcaccttaa accacacgga taaagacgcg
     1261 actatttccg cggatattgt cagtggcagc gagggcctgg gctatatcaa tgcgctcaat
     1321 ggcacgactt acttaaccgg tgataactct gcctttagtg gtaaagtcaa aattgagcaa
     1381 aatggcgctt tagggatcac ccaaaatata ggtacagcag agatcaacaa ccgcgggaaa
     1441 ttacacctga aggctgacga tagcatgacc tttgccaata agatctctgg caacggtaca
     1501 ataagtatcg acagtgggac ggtggagttg accggcaata actatgcatt cagcggatat
     1561 attgatgttg cttctggtgc tgtcgctgtt atttctgaag acaagaatat cggtcgtgca
     1621 gagctggatg tcgatggcaa attgcaaatt aatgccaaca aagattgggt atttgataac
     1681 gatcttgaag gtagaggcat tgttgaaata aacatgggga atcacgaatt ctccttcgat
     1741 gagtttgctt atacagactg gttccagggt tcactggcgt tccagaacac gacatttaat
     1801 ctggaaaaga atgctgagtt tctgcagaaa ggcgggatca ctgcgggtca gggaagcctg
     1861 gtaacagtgg gtaagggcgc tcactccatt agcactttgg gattctccgg cggaaccgtt
     1921 gattttggtg ccctgacagc aggtgcacag atgacagaag ggacggtcaa cgttagtaaa
     1981 acgctggatt tgcgcggcga gggtgtgatt caggtttctg acagtgacgt tgtccgctca
     2041 gtatctcgtg atattgactc tgcgttatcg ctcactgaag tcgatgatgg taacagcacc
     2101 attaagttgg ttgatgcgca aggtgcggaa gttctgggcg atgcgggcaa tctgcaattg
     2161 caggataaaa atgggcaaat cctctccagc agcgcccaac gtgatattca gcagaatggg
     2221 caaaaagcgg ccgtcggcac ttacgactat cgtctgacga gtggggtaaa caatgacggt
     2281 ctgtatattg gttacggcct gacccagctt gatttacacg ctaccgacag cgatgctctg
     2341 gtgctgagct ctaacggtaa aagcgagaat gccgccgatc tcagcgcaaa gattaccggc
     2401 agtggtgacc tggcattcag cagccagaag ggtcagaccg tatcgctttc taacaaagac
     2461 aacgactata ccggtgttac cgatctgcgc agtgggacgc ttttgttgaa taacgataac
     2521 gtgttgggta atacccatga actgcgtctg gcggcagaga ctgaactgga catgaatggt
     2581 cacagccaga ccgtgggcac gctcaatggc agcgccgatt cactgctgag cttaaatggc
     2641 ggcagtctga cggttaccaa cgggggcact tcaaccggtt cgttaacggg gagcggagag
     2701 ctgaatattc agggcggcac gctggacatc gcgggcgata acagcaacct gacggcgaat
     2761 gtgaacattg ctaattcggc taatgtcctg gtaagtcatg cgcagggatt gggtagcgca
     2821 aacgttgaga acaacggtac cctggcgttg aataatagcg ctgaaaaaag agcggctgcg
     2881 tctgtgaatt acgccctggg cggcaatctg accaacaacg gtacgctgat gaccggaatg
     2941 tcaggacagc aagctggcaa tgtgttagtg gtgaagggga actaccacgg taataacggt
     3001 caactagtaa tgaatacggt actgaatggc gatgactcag taaccgataa attggttgtc
     3061 gagggcgata ctagcggcac gactgccgtt acggtgaata acgctggcgg tacaggtgcg
     3121 aaaaccctta acggtatcga acttatccat gtagacggta agtctgaggg cgaatttgtt
     3181 caggctgggc gtatcgttgc gggggcgtat gactacactc tcgcgcgtgg acaaggggca
     3241 aatagtggta actggtatct gaccagcggc agtgattctc ctgaactgca gccggagcca
     3301 gacccgatgc cgaatccaga gccaaacccg aatccagagc cgaaccctaa cccgacacct
     3361 acgccgggtc cggatctgaa tgtggataat gacctgcgac cggaggcggg tagctacatt
     3421 gcgaaccttg cagcagcgaa taccatgttc accacgcgtc tgcatgagcg tctgggtaat
     3481 acgtactata ccgacatggt gacgggtgag cagaaacaaa ccactatgtg gatgcgccat
     3541 gaaggtggtc ataataaatg gcgtgatggc agcggccagc tgaaaaccca aagcaatcgc
     3601 tatgttctgc aactgggagg cgatgtcgcg cagtggagcc aaaacggcag cgaccgctgg
     3661 catgttgggg tcatggcggg atatggcaac agcgacagca aaaccatttc ctcgcgaacc
     3721 ggttatcgtg caaaagcgag tgtgaacgga tatagcacag gcctctatgc cacctggtat
     3781 gccgatgacg agtcgcgtaa tggcgcgtat ctcgacagtt gggcgcagta cagctggttt
     3841 gataacacag tgaaagggga tgacttacaa agtgaatcct ataaatcaaa aggatttacc
     3901 gcttcactgg aagctggata caaacacaaa ttagctgaat ttaatggcag ccagggaacg
     3961 cgtaatgaat ggtatgttca gccgcaagca caggttacct ggatgggagt caaagccgat
     4021 aagcaccgcg aaagcaacgg aaccctcgtt catagcaacg gtgatggcaa tgttcaaacc
     4081 cgacttggcg taaaaacctg gctgaagagc caccataaaa tggatgacgg taaatcccgc
     4141 gagttccagc cgtttgtaga agtgaactgg ctacataaca gtaaggattt cagcaccagt
     4201 atggatggcg tgtctgtcac tcaggatgga gcccgaaata ttgctgagat aaaaaccggg
     4261 gtggaaggac agctaaatgc caacctgaat gtctggggga atgtgggcgt tcaggttgcc
     4321 gataggggat ataatgacac ctctgcaatg gttggcatta agtggcaatt ctgatctgaa
     4381 taatgagtaa aaggagagag agccgaacgg gagataattc ggctctctgt gtcattttct
     4441 tgcagccagc tttatccgtc agagtgaaga ctcgcagatt tagctggcgc acaagattta
     4501 atcaaggtgt gttcaatggc ttcgcatgat ttgagtgttt ttttggaaga gttcggggca
     4561 acggtgaatt tgacgttgcc tggtatcgtg tccgaaaaag aacggctttt actcaagctg
     4621 ctaatggagg gaatgtcggt tacagaaatc tcacagtaca gaaatcgcag tgcaaagaca
     4681 atttcacatc aaaagaagca gctgtatgaa aaactaggca tccagagtga tattaccttc
     4741 tggcgggata tcttttttca atatcatccg caagtcatta gtggcacggg aaataaaaat
     4801 aatttttata tccctgataa ccgttgtcac catatcgtca cgcctgaagc catcagtctg
     4861 gcgttggaaa accatgaatt taaaccgtgg atccaaccgg ttttctgcgc gcagactggg
     4921 gtactgacgg gctgtgaagt gcttgtccgc tgggaacatc cacaaacggg aatcatcccg
     4981 ccggatcagt ttattcctct ggcggagtca tccggtctta ttgtcataat gacccgccaa
     5041 ctgatgaaac agactgcgga tattctgatg ccggtaaaac atttgctgcc ggataatttc
     5101 catattggta tcaacgtctc ggcgggttgt tttttggccg cgggatttga aaaagaatgc
     5161 ctgaacctgg tcaagaaatt aggaaacgat aaaatcaaac tggttctcga gctaacggaa
     5221 cgtaacccta ttccggtaac gccagaagcc agagcgatat ttgacagcct tcatcagcat
     5281 aacattacct ttgcgctgga tgactttggt acgggttatg cgacctatcg ttatctgcag
     5341 gcgtttccgg tcgattttat taagatcgat aagtcatttg tgcaaatggc gggtgtcgac
     5401 gaaatatccg gtcatattgt ggacaatatt gtcgaactgg cgcgtaagcc tggtctgagt
     5461 atcgtggcgg aaggggtaga aacccaggag caggcggatt taatgatcgg taaaggcgtt
     5521 cactttttgc agggctattt gtactctccg ccagtaccgg gtaataaatt tatctctgaa
     5581 tgggtaatga aagcaggtgg ttgatgtaaa ccgctattca cagcgcatcg ggaggttggc
     5641 agcgattaat tctcccgatg cagtaaatcc tgatagatcc ccgttaatat gccatttggc
     5701 gcaaattgtt ttttaatcca ctgcgtcacc tgacccgtag cagaatgctg agtcgccagt
     5761 aacatgcgtg aatcctggcg cgggttatgg atttgtcggg taaccagcag ggattgcgcc
     5821 atcgcctcac ggaccatgta atctggcaaa aaaacaatcc cttcacctaa aatctggcac
     5881 tgacatttgg tgttgaaatc tggcaccaga attgactcct gcccgtgcag caaccagccc
     5941 acttttttat taatcgtatg cgcggtgtcc tccaccataa tattagggta gagacgcagt
     6001 tggctttcgg cgatggttcc ggcacaaagg ctaacgggtg atccggggcg atagcaaaag
     6061 cccagcgaat cgcgccaatt tcagtgtaat caataccgcc gccgtccagc agcgtgtcgg
     6121 gtgcgccgat agcgatattt gcctgattat taataatcgc atcccagacg ccgttataca
     6181 cttcggtggt gacggtaatc tggcaggtag gaaactgctt tttcagcacc tgtaataagc
     6241 gtgcggtgtg tttgggcgta taaagcaact gattaataca aatgcgcaca cgcgcttcta
     6301 tgccctgcga aatagtatcg atgctgcgtt tgatggcgtg aaaatcattc agcaggtcgg
     6361 tagctttgcg gaaaaaataa cgcccggact ccgttaactc aatgctgcgg gtactgcgag
     6421 tgaacagcac cacatccagc cctgtttcca ttcgcttgat ggtgtagcta atggcggaag
     6481 tggttaaacc caactcttct gcggctttac tgaaactgcc aaaacgcgca gcggtagtaa
     6541 atgccaacag attctcttcg gtaaaaattg agttcattaa accattcccg ccattatcta
     6601 atatgaattt ctccaagcat aactgtagag gtatcaatta gagatgggta atcatttttg
     6661 aataactttt aatacccgtc gcgtttagtc gtcgcttcgc ttaatttttg tccgccatat
     6721 tctgaaatta tccccacgca gttacccgcc agaaaaccca gtaacgtcac cgtccagttc
     6781 atcccgctgg caaaaaacag cgtcatgccg agaaagccac cgggaataaa tgacagtaac
     6841 cagaaacgcc cttgccagac caccaccgcc gagaagggta cggtagtaat tacgctcgcc
     6901 cagaacgcgg acagtccact tacagaggcc agccaaccgg cagccagcgc ggcgaaaaac
     6961 gcccacacca tcccggaata attaaccgac agacttctga taaaccccga tttaccgccg
     7021 cctgcggcat aaaaactgca aaaggcgacg aagccaatgg ttcctaataa ttcctaataa
     7081 ttcccatccc tgagagagcc cgacatgtga agacactaac tgccaaagcc ctgcacaaat
     7141 accgacggtg acacccgttg ccgtgagtcc gttcatcttg ttcccctctg gttagacatg
     7201 gacgaaagct actcttacat gtgcagacta tagagaaata aaacctgatt gagtgaaagg
     7261 gtatcgggtc aaagaaacaa atattgcact accgcacact gcgaaaagat tgttgaacat
     7321 cattcaacaa aaaggcgaaa tacggcgaat taaatttaag cgggttcttt tatcagatta
     7381 tcgttgctat tctcaattca tcgaaaacag ccacaaaaac caggggaatg tgaatgagta
     7441 taaaaaatct acctgccgac tatttattgg ctgcacaaca gggtgatatc gataaagtaa
     7501 aaacctgcct tgcgctgggt gtcgatatta atacctgcga tcgtcagggg aaaacggcaa
     7561 ttacgctggc aagtttatat cagcaatatg cttgcgttca ggcattaatt gatgccggag
     7621 cggatattaa taagcaagat catacctgtt taaatccttt tttaattagc tgtctgaacg
     7681 gtgatttaac gctgctacga attattttac cggctaaacc agaccttaat tgcgtcaccc
     7741 gttttggcgg tgtcggcctg acgcctgcct gtgaaaaagg ccatttaagt attgtaaaag
     7801 agcttttggc gcatacggaa attaacgtta accagaccaa ccatgttggc tggacgccac
     7861 tactggaagc gattgtgctt aacgatggcg ggattaaaca gcaggcgatt gtgcagttat
     7921 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
     7981 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
     8041 ccgggaggct tgctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
     8101 cggattttct ggcgcagcga gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
     8161 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
     8221 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
     8281 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
     8341 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt cgccttatcg
     8401 cctggcaaca gtggcacgac atcattcatc agcacctggg ggaaaatgaa accctgttta
     8461 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
     8521 gggcagttat acaggcagta atcgataaac aaaatatcgc ctcagcggtc gccagtatga
     8581 tgggcttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcactta
     8641 ttttatttat ttccgggcat ccggcgggaa aatacaaaga ggaattttat ctcggcttgc
     8701 aacgcggcag aaataatacc acgttattaa gtgccataac gctggaagcc gcattacaac
     8761 aacgctgccg ggaaaatatt catcgtttta ttcacaacat tatttatgac atccctggga
     8821 acgcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     8881 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     8941 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     9001 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     9061 aaaggtgtgc tgaaaaattt aggactgctg acgccggagc tggagcaggc gaaaaacggc
     9121 gacctgatga ttgtcatcaa tggtaaatcg ggggcggaca acgagcagtt gctggtggag
     9181 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     9241 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     9301 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     9361 tccgataacg tctcagttga agatgaactg gcgctcaagc aactggccca cgaaaaaggg
     9421 ctgctgatga tggggccaga ctgcggcacg gcgattatca acggtgcggc gctctgcttt
     9481 ggtaacgccg tgcgtcgcgg caacatcggt attgttggcg catccggcac cggcagtcag
     9541 gagttgagcg tccgcattca tgaatttggc ggcggcgttt cgcaactgat tggcaccggc
     9601 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     9661 gaaaacgatc cgcaaactga aatcattgcg cttatctcca aaccgcctgc gcctgcggtg
     9721 gcccgcaaag tgctggaacg tgcgcgcgcc tgccgcaagc cggtggtcgt ttgcttcctc
     9781 ggtcgtggcg aaacgccagt ggatgagcag gggctacagt ttgcccgcgg caccaaagag
     9841 gcagcgctaa aagcggtgat gctctccggc gtgaaacaag aacatctcga cctgcatacc
     9901 ctgaaccagc cgttgattac tgacgtgcgc gctcgcctga agccgcagca gaaatacatt
     9961 cgtggcctgt tctgcggtgg cacgctgtgc gatgaaacga tgttcgcggt gatggaaaaa
    10021 cacagcgatg tctacagcaa catccagccc gatccggaat tccgcctgaa ggatatcaac
    10081 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcaccaa cggcaagccg
    10141 cacccgatga ttgaccctac caaccgcatc agccgcttga tcgaagaggc gcgcgatcca
    10201 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
    10261 ggttccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
    10321 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
    10381 agccagatgc tgcttgatgc cggcgtgatt ctggcgagca gcagcaccaa taccggattg
    10441 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
    10501 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
    10561 atgtagaagt gactcaattc gactggacgc cgccggggca gggcaatatg caggtggtgc
    10621 aggcgctgga taacattgcc gattcgccgc tggcagacaa aatcgccgcc gctaaccagc
    10681 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
    10741 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
    10801 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
    10861 cgaaagatct cgacgaggcg gctgaactgg cggcttccgg ggagatcacc ttctcgccgt
    10921 gtcacgagca cgactgcgtg ggatcgatgg cgggtgtgac ctcggcctcg atgtttatgc
    10981 acatcgtaaa aaacaaaacc tacggcaaca tcgcttatac caacatgagc gagcagatgg
    11041 cgaagatttt gcgtatgggc gctaacgacc agagcgtgat tgaccgcctg aactggatgc
    11101 gtgatgtgca gggaccaata ctgcgcgacg cgatgaaaat tatcggcgaa atcgatctgc
    11161 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
    11221 ggacgacgct gctgattcag gcgctgacgc cggggattat ccaggcgggc tattccgtcg
    11281 cgcaacagcg cgaagtgttt gagtttgttg ccagtagcga ttacttctcc ggcccgacgt
    11341 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtcg
    11401 ttaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtgagcggc ctgccggggc
    11461 agtggtttac cggcccagcg cagcaggtca tcggcccgat gtttgctggt tataagccgg
    11521 aagattcggg gctggatatc ggcgacagcg ccatcacaga aacctacggt attggcgggt
    11581 ttgccatggc gaccgcgcct gcaatcgtcg cgctggtggg gggcacggtg gaagaggcta
    11641 tcgatttctc ccgtcagatg cgcgaaatca cccttggcga aaaccccaac gtcaccattc
    11701 cgctgctcgg ttttatgggc gtgccgtcgg cagtcgacat cacccgcgtg ggtagcagcg
    11761 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
    11821 gcgcgggcat tgtgcatcca ccattcgcct gcttcgagaa agccattctt ggctggtgcg
    11881 aacgttacgg cgtttgacct acctgttcat accggatgcg gcgtgaacgc cttatccggc
    11941 ctacgaatgg cgcaagaatc tgtaggcctg ataagcgtag cgcatcaggc atttgtcacc
    12001 attgccggat gcggcgtgaa cgccttatcc ggcctacaag tggcgcaaga atttgtaggc
    12061 ctgataagcg tagcgcatca ggcatttatc accattgccg gatgcggcgt gaacgcctta
    12121 tccggcctac gaatggcgca agaatctgta ggcctgataa gcgtagcgca tcaggcattt
    12181 gtcaccattg ccggatgcgg cgtgaacgcc ttatccggcc tacgaatggc acgagaattg
    12241 taggcctgat aagcgtagcg catcaggcat ttgtcaccat tgccggatgc ggcgtgaacg
    12301 ccttatccgg cctacgaatg gcacgagaat ttgtaggcct ggtaagcgta gcgcatcagg
    12361 cagtctggcg ttggtcataa ccccatcacc ctctgtagcg gacataacaa catgaaagaa
    12421 cttgtggtcg ttgccattgg tggcaacagc attatcaaag ataacgccag ccagtcgatt
    12481 gagcatcagg cggaggcggt gaaagccgtg gccgatacgg tgctggaaat gctggcttcc
    12541 gattacgaca ttgtgctgac ccacggtaac gggccgcagg tcgggctgga tttacgccgt
    12601 gcggagattg cccacgagcg cgaagggctg cccttaacgc cgctggcgaa ctgtgtggca
    12661 gatacgcagg gcggcattgg ctacctgatc caacaggcgc tgaacaaccg gctggcgcgt
    12721 cacggcgaga aaaaagccgt caccgtggtg actcaggtgg aagtggataa aaacgatcca
    12781 ggttttgccc atcccaccaa gcccattggt gcattcttca gtgaaagcca gcgtgacgaa
    12841 ttacaaaagg caaaccctga ctggcgtttt gttgaagatg ctggacgggg ctatcgccgc
    12901 gtggtcgcct cgccggaacc gaaacgtatt gtcgaagcac ccgccattaa ggcgctgatc
    12961 caacaaggct ttgtggtgat tggcgcgggc ggcggcggaa ttcctgtggt gcgtactgac
    13021 gcgggagatt accaaagcgt ggacgcggtt atcgacaaag atctctctac cgcgctgctg
    13081 gcccgtgaaa ttcacgccga cattctggtg atcaccaccg gcgtcgaaaa agtctgtatc
    13141 cactttggta aacccgagaa gcaggcgctg gatcgggtgg atattgccac catgactcgc
    13201 tatatgcagg aaggacattt cccgcccggc agcatgttgc caaaaatcat cgccagcctg
    13261 acgttcctgg aacagggcgg taaagaagtg attatcacca cgccggaatg cctgccagcg
    13321 gcgctgcgcg gtgaaacggg cacccacatt attaaaacgt aaggacgtaa gatgaaagaa
    13381 aataatagcc gccgtgaatt tctgagccag agcggtaaga tggtcaccgc cgccgcgctg
    13441 tttggtacct ctgtgccgct cgcccatgcg gcggtctctg gcaccacaaa ctgcgaagcg
    13501 aacaacacca tgaaaatcac tgacccgcat tactatctcg acaacgtgct gctggaaacc
    13561 ggttttgact acgaaaatgg cgtggcggtg cagacccgca cggcgcgcca gaccgtggag
    13621 attcagaacg gcaaaattgt cgcgctgcgc gagaataagc agcacccgga tgccacgctg
    13681 ccgcactatg acgctggcga taagctgatg ctgcccacca cccgcgacat gcatattcat
    13741 ctcgacaaaa cgttttacgg cgggccgtgg cgctcgctca atcgcccggc aggcaccacc
    13801 atccaggaca tgatcaaact cgagcagaaa atgctgccgg agctgcaacc gtacactcag
    13861 gagcgggcag aaaaactgat tgatttattg cagtcgaaag gcaccaccat tgcccgcagc
    13921 cactgcaata tcgaaccggt ttccggcctg aaaaatctgc aaaatttgca ggcggtgctg
    13981 gcgcgacgtc aggcgggctt tgagtgtgaa atcgtcgcct tcccgcagca cggtttgctg
    14041 ctgtcgaaat cggaaccctt aatgcgtgaa gcaatgcagg cgggtgcgca ttacgtcggc
    14101 gggctggacc cgaccagtgt cgatggcgcg atggaaaaat ccctcgacac catgttccag
    14161 attgcgctgg actacgacaa aggcgtcgat attcacctgc acgaaaccac tccgtcgggc
    14221 gtggcagcca tcaattatat ggttgaaacg gtagagaaaa cgcctcaact gaaaggtaag
    14281 ctgaccatca gccacgcctt tgcgctggct acgctcaacg agcaacaggt agatgaactg
    14341 gcgcatcgca tggcggcgca gcaaatttct atcgcctcga cggtgccgat tggcacgctg
    14401 catatgccgc tcaaacagtt gcacgacaaa ggcgtaaaag tgatgactgg cactgacagc
    14461 gttatcgacc actggtcgcc ttatggtctg ggcgacatgc tggaaaaagc caatctctac
    14521 gcgcagctct atattcgtcc taacgaacag aacctctccc gttcgctgtt tctagccact
    14581 ggcgatgtat tgccgctgaa tgaaaaaggc gagcgtgtat ggccaaaagc gcaggatgac
    14641 gccagctttg tgctggtgga cgcctcctgt tccgccgagg cggtggcgcg tatctcgccg
    14701 agaaccgcaa cgttccataa agggcaactg gtgtggggga gtgtggcagg ttgatgcgca
    14761 tttgaaacgc ctgcaacggt gaatagtaag agatttaagc cccagggagt ggggcgggaa
    14821 tggtttgcta atgaatgatc ctgaacttta taatctcccg gcataatttc caggggatta
    14881 tttgagagaa atacacaaaa gtgagtttta acaaaggtat ttatcaggcc ataaatgtga
    14941 cctgcttcgg ataaaaaaga ttcagtatcg aaaaaccaat gatactcttg ctgatggtta
    15001 ccaacagtaa ttaagatgtg atttgttttt ccgtaatcgc aaaaatattg ccatgaccat
    15061 aaaagaaaaa ttctcacaaa aatatcccca tgcttccttt tgtacttttg gcgattcagc
    15121 cgcgttggcc gatcacctcg caacgttgat tgcgactggt gttaaaacag cttcctgtgg
    15181 ttcgctggcg ggatgtattg aagacaacgc atttccgatg attggtgagt ataagattgt
    15241 agaaaatagc cgtggtgagc ctgtatgtgt tatcagagtg attggcctgc atttactgcg
    15301 cttttctgat gtcaccgcgg agcttgcccg taaggaaggt gaaggtgacc tcagtcttga
    15361 atattggcgt aatgaacacc gtcgtttttt ccaggcagaa ggaagctatt caccagaaat
    15421 ggacgttatc tttgaagaat atgcccttat tgacgttgtg tagataacta tttatctaca
    15481 tgaaataagc tcacaaacat aaaatatcgc taattttgtt tattttgctg ttttttacgt
    15541 gatgaatatc acacttatta ttgctgcccg ttttgtccat atttataacc atatttttaa
    15601 tagctgcctg aaatagagct gtttattctg cataaaaata ttcgaaaatt cttattctct
    15661 ccagcgcgct aatttgggcg aacacttcct gactaccctg caatgaggct gaaatgatga
    15721 ataaacgttt tgttatcaat atggtgtctt cgctgttatt aggcgctgcg ctaatttctg
    15781 cgccgctcca ggcagcggaa aaagtcgtgg tgaatatttc taaagtcgat ggtatgccgt
    15841 ggtttaatcg catgggcgaa ggtgtggttg aggcgggtaa agcctttggt gttaatgcct
    15901 cacaggtggg gccttcaagt actgatgctc cgcaacaagt taaaattatt gaggatttaa
    15961 tcgcccgtaa ggttaatgca attactatcg tgccaaacga tgccaacgtg ctggaaccgg
    16021 tattcaaaaa agcgcgagat gccggaattg tggttctgac caacgaatcc cccggtcagc
    16081 caagtgctaa ctgggatatc gaaatcattg ataacgaaaa atttgccgct gaatatgttg
    16141 agcatatggc gaaacgcatg ggtggcaagg gcggctatgt gatttacgtg ggtagtttga
    16201 ccgtgccgca gcataactta tgggctgact tgctggtgaa ataccagaaa gagcattacc
    16261 cggatatgca tgaagttacc cgccgcatgc cggttgccga gagtgtggat gtctcacgtc
    16321 gtactacact ggacctgatg aaaacctatc ctgacttgaa agcggttgtc tcctttggtt
    16381 caaacggtcc gattggcgcg ggacgtgcgg tgaaagagaa acgggcgaaa aataaagtcg
    16441 cagtctatgg catgatgatc ccttcacagg cggcatcact gattaaaagt ggtgatatca
    16501 ccgaagggat tacttacgat ccggcctctg caggttacgc actcgcagcc gtcgccagca
    16561 cgttgctgaa gggtgaagag atcaagcctg gattagaaat gcagaatctg ggtaaggccg
    16621 acgtagatat ggataagcga attatccgtt tccataaggt attactggtc aacaaagaca
    16681 atatcgactc actttattaa tccatttctt cggggcgaaa gccccgattc ttattgccag
    16741 gggatgttat ggaaaccttc ctttcccttc gtcatatcaa caaaacgttt catgccacgc
    16801 gagcgctgcg cgatgtttct ctcgacttta tgtcgggtga agtgcactgc cttgccgggc
    16861 aaaacggctg tggtaaatcg acgttgatta aaattatgtc cggtgtttat cgcccggatg
    16921 agggagcgga aattacgctt ggtgggaaaa actggtcaaa gctgacaccc gccgcttcgg
    16981 tggcgcaggg gattcaggtg atttatcagg acctctcttt atttcctaac ctgagtgtct
    17041 gggagaatat cgccgtgaat cactatcacc acggtctgtt tgttaaccgc cgtcgtctgc
    17101 gtgaggtggc gcaggcggca atgactagta ttaacgtcac attgccactg gatacgctcg
    17161 tttctgagct ttcaattgcc cgctgtcagc tggtggcaat ttgccgtgca ctggcgcagg
    17221 acgcacggtt gatcgtgatg gatgaaccaa ccgcttcgct gacccatcag gaagtgcaag
    17281 ggttattaca ggtggtgcat caattgcgtg aacgcgggat ctgcgtggtc tttgtcagtc
    17341 atcgtctgga agaagtgatg gaagtttccg accgtatttc agtgctgaaa gatggtgagc
    17401 tggtcgggac ttttccggct gcagagatga ccacaaaaca actcggtttc ctgatgaccg
    17461 gccaggagtt tgaatatcag gtgcgagagt tgtggcaggg aaaatccagc accccggtgc
    17521 tggaggtgcg taatttaagt cgtcatgggg aatatctaaa tattaacttg cgggtggaag
    17581 cgggcgaagt ggtatcgatt gttggcctgc ttggtgcggg gcgcacggaa ttatgtctga
    17641 gtctgtttgg tatgacccga ccagatgctg gcgagatcct catcaacggc cagctggtta
    17701 cactgcatag taaccaggac gccatccgcc acggtattgg ttatgtttcg gaagatcgca
    17761 tgtcgcgcgg tttggtgatg gcgcaatcta ttgaagacaa catcattagc actgtttttc
    17821 acaaggtaaa agatcgcttc ggctttctga gtgaaactaa agtgcgtgac ctggtggaca
    17881 ggctaatcaa ggcattgacc atcaaagtct ctgatcctca tcttccggtg aatacgcttt
    17941 ctggtggtaa cgcgcagcga gtatcgatcg ccaaatggct ggcgattggg ccacgactgt
    18001 tgattctcga tagcccgaca gtcggtgttg atatcgccaa caaagcagga atatacggca
    18061 ttatcagcga tctggcggct catggcattg cggtattgat gatctgcgat gaaattgaag
    18121 aagcgtggta tcaaagccac cggattttgg taatgcaaaa aggtcagatc acccatagct
    18181 tcctgcctga cagcagttct caggccagga ttgcggaggt ggtaaatggc tgaactgaaa
    18241 aaacgccatg aattctggct ggcattactg attgtggtgc tgtttgttgg ccttgcctgg
    18301 cgcagcgacg agtttctgac attcggtaat ttgtacgatc tcgccaataa ctatgccatg
    18361 ttgactattc tcgcctgtgg tttgtttgtg gtgttgattt ccggtggaat tgatatttcg
    18421 tttccagcaa tgaccatcat tgcgcaatac ggcatggtgc tgttgctgca aaaaattggt
    18481 ggcaacttcg ctgtcgcgtt tgcactggca ggttgcatcg gcattttact tggcttaatt
    18541 aacgccttac tggttaatcg cctacgggtg ccttctatca tcatcactat ctcgacgctg
    18601 aatattttct atggcctgct gttatggttg agtaaaggtg tgtggctgta cgactttccg
    18661 ccgtggtttg agcagggggt tatgttgttc aagtacaccg atgctgatgg ctatgactat
    18721 ggccttggtc tgccgctgat cgccatgatt acggtggtgc tgctgacagc gtttatcatg
    18781 aatttcacca gtgtagggcg caaaatttat gcccttggcg ggaaccgcga atcagccagt
    18841 cgcatcggct ttagcgtgct gaaactgcaa cttttcgtct acggctatat gggattgatg
    18901 tctggcgctg cgggtgtagt gcagtcgtgg acggtgatga ctgtcgcccc cgattctctt
    18961 ctgggttatg agctgacagt actggctgcg gtggtgcttg gcggcactag tttgctcggc
    19021 gggcgcggca cgttaactgg tactttgctc ggcgtggtgt tgttggcagt gatgcaaaac
    19081 gggctaaatt tattgggagt ctcgtcttac tggcaaacat tgatcaccgg catcatcatc
    19141 gttgccagca ttagcgccac ggcgtggagt cagcatcaga accggagcct gctatgaaaa
    19201 aatcgtggcg taataacgtt gagttttact tgatcggcct gctggtgcta actgttgccg
    19261 cgttcagcat tactatgccg gagattttct ggtcgataag taatttccag tccgtcgctt
    19321 cgcaaatgcc cgtgctgggc attctggcgt tggctatggc ggtgaccatg ctttgcggtg
    19381 gcattaactt gtcaattata gccactgcta acgcctgttc actggtgatg gcgtgggtgg
    19441 cgactcagta tccaccgggt attgctacgg ttgtcgctac gttactggcg ggcgctggcg
    19501 cggcggtgat aatcggttta tgtaacggcg tattgattgc tggtattcga gtgttgccaa
    19561 ttcttgcaac gcttgggatg atgacgttgc tcaaagggat aaatattctg gtgacaggtg
    19621 gtagcgccat tgccaattat ccctcatggg tgctgtggtt gaatcatgcg caatggttcg
    19681 gtattccgct accgatgtgg ttgtttgccg cagtggcact ggggctgtgg atattgctgg
    19741 agaaaacgcc gctcggtaaa gccatttacc tgattgggtc taacgaacga gcaacacttt
    19801 actccggcat taacactcgt cgggtgctga tttgggttta tgtcatttcc gccttgcttt
    19861 gtgcggtagc ggcatttttg atgatgtcga aactcaactc ggctaaggcc tcttatggcg
    19921 aatcgtactt gctggtgtca atcctcgcgg cagtactcgg cggggttaat ccggacggcg
    19981 gcagcgggcg gataattggt atggtgctgg cgctgtttct tctgcaaatt attgaaagcg
    20041 gcttcaatat tctcggtata agcccctacc tgacgatggc cttatgggga acgttgctgc
    20101 tctgtttcat tcaggctcgc ggcatgttgg ggctggatcg ggtggtttaa atattggcta
    20161 tattcaatgg acgcgttttg ccgcgatgac atatcaggcg tcgccaaata cacatagcta
    20221 atcaggagta aacacaatga agatcaaagc tgttggttca tattccgcta aacaaccact
    20281 tgaaccgatg gatatcaccc ggcgtgaacc gggaccgaat gatgtcaaaa tcgaaatcgc
    20341 ttactgtggc gtctgccatt ccgatctcca ccaggtccgt tccgagtggg cggggacggt
    20401 ttacccctgc gtgccgggtc atgaaattgt ggggcgtgtg gtagccgttg gtgatcaggt
    20461 agaaaaacat gcgccgggcg atttggtcgg tgtcggctgc attgtcgaca gttgtaaaca
    20521 ttgcgaagag tgtgaagacg ggctggaaaa ctactgtgat cacatgaccg gcacctataa
    20581 ctcgccgacg ccggacgaac cgggccatac tctgggcggc tactcacaac agatcgtcgt
    20641 tcatgagcga tatgttctgc gtattcgtca cccgcaagag cagctggcgg cggtggctcc
    20701 tttgttgtgt gcagggatca ccacgtattc gccgctacgt cactggcagg ccgggccggg
    20761 taaaaaagtg ggcgtggtcg gcatcggcgg tctgggacat atggggatta agctggccca
    20821 cgcgatgggg gcacatgtgg tggcatttac cacttctgag gcaaaacgcg aagcggcaaa
    20881 agccctgggg gccgatgaag ttgttaactc acgcaatgcc gatgagatgg cggctcatct
    20941 gaagagtttc gatttcattt tgaatacagt agctgcgcca cataatctcg acgattttac
    21001 caccttgctg aagcgtgatg gcaccatgac gctggttggt gcgcctgcga caccgcataa
    21061 atcgccggaa gttttcaacc tgatcatgaa acgccgtgcg atagccggtt ctatgattgg
    21121 cggcattcca gaaactcagg agatgctcga tttttgcgcc gaacatggca tcgtggctga
    21181 tatagagatg attcgggccg atcaaattaa tgaagcctat gagcgaatgc tgcgtggtga
    21241 tgtgaaatat cgttttgtta tcgataatcg cacactaaca gactgaaaaa attaataaat
    21301 acgctgtggt ttaacatatt aacttcgctc tccacttaac tttttagtta aggagagcga
    21361 aataatatca aagtagcagt aaaacctata acgtaaattc aaattgttaa attaactccc
    21421 tccagtacct aatacttcac acgttagtta tgagcgattt ctgatagtgc ctggtttaat
    21481 cagagcttta ttatctgcga cgtttatttt tatttaagag agatcgcgat gatatcatta
    21541 aaagctccgc acaataattt gatgccatat acgcaacaaa gcatacttaa tacggttaaa
    21601 aacaatcagt taccagagga tatcaaaagc tccctggttt cctgtgtcga tatattcaag
    21661 gttttgatta aacaatatta tgattatcct tatgattgtc gtgatgattt agtcgatgac
    21721 gacaaactca ttcatctcat ggctgccgta cgagactgtg agtggtcaga cgataacgca
    21781 ctcaccataa atgtgcagtt taatgatttc cctggatttt atgactggat ggattaccct
    21841 gatcacccgg ttaagtttgt ttttcgcata cttgaaaatc aaaaagggac agtttgggta
    21901 tatgaccagg atgatgcgtt tcttgatatt aaagcaaatg tgcaggctgg acgctttacc
    21961 gggcttaaga aattagtgca gtttatcaat tcagtgcgaa cagattgcaa atgtattctt
    22021 cttgaacacc atatgccttt gctgagactt ttccctaaag ggaaagagtg tatgcatgtg
    22081 gaaaaatggc ttcgcgaaat gtcgtctatt ccggagacag acgcgcccat taagcaggcg
    22141 cttgcgcatg gattattatt acacttaaaa aatatctacc ctgtttttcc tgaatcactg
    22201 gtgatgttat tgctctctgt tctggacgta aaaacataca gagatgatgc acgattaaat
    22261 gaagggatat ctaatagagt ccaggaatta ggtgacagat attatcctgt aaataagcac
    22321 gttaaaatcc gctacactct ttagttcaga aagggttcca cctgcaagtt gttattctac
    22381 ctgagagtga atcgcaatgc aggtaatcat ttcatctgta tatctgtatg ccaactggca
    22441 ttccatctct tttttcgccg atattttgtt gtgatcggcg acacttcgct taaaaaagca
    22501 ccagtagtgg tttcgcagcc atgcggtgta taaaaaaaaa tgatctcatg cagatgtttt
    22561 gtgaatgtgt gggttgacat tcatatgaaa aaaatcataa ttccatcatg tttgtgtggg
    22621 gtcttttctg tatcttacgc atcgcactca agcctgacag aaaatatgct gtaaggctca
    22681 tatcaaaaac cgccattagc tcatcaggaa gagcagacga caaccaggat tgttgtatgg
    22741 tacggggttc gaggccccga tggcggtcca acttttcaaa actcttctga attagatccc
    22801 cttattatca tccctgcaaa gtggaacatt gccattgttt ggctattcag gacgggtcag
    22861 accaatgaaa aaagcccaca caggggagag tgggctgaaa tgggaagcta aagactcaag
    22921 taaacttatc ggaaataagg accacgcatt acgggggcta tcatcgccga tggtcgggat
    22981 tcggcaaatc aggttgatta cgtagagagt aaattatctg ctcaccgctg cgtcacccct
    23041 tcgtaaatca ggcgtagcgc gaatacacca ataattgcac caataacccg actggcaacg
    23101 cgttgcatac gcccataagc acgacgcaca gcgggcaaag aaaacgcctg actaagaaaa
    23161 actcgccaga taattgatgc gagcacaatc cccgcccagg ccattaaacg tgcccatgtt
    23221 ggtgtttcgg catttaatgt tactgagaaa atactaataa aaaataaaac ggtttgtggg
    23281 ttagagagat cggtaattaa tccacggcga aaaaagacat accagggggc gttaatcggt
    23341 tgttgtagtg tgctcatttg cggtgttgac tggcggcgca tgctgcacca cgcaaaccat
    23401 aagagataag cgccgccgac gattctgata agcgaaaaaa tctcctcaca ctgcgtaatt
    23461 agcgttgcaa gaccaaacaa acccaacccg gaataaaatg catcgcccag cgccacgccc
    23521 agtccggtca gcactcctgc gcgtcgaccg gaagccaggc tggtttgtac taccacaaaa
    23581 agattggctc ccggattaaa aaaagtaatc acgaacagtc ctacggtcag gtaaacggca
    23641 tgcaaaggat ccatagtgat ttcatccata aataagtgaa ctaactgcat catatttcta
    23701 ccaaaaataa tcgggtgcga gagagatcac aaagtgtctt atttccggtt actggcgttt
    23761 atgccctgac tgaactaatt attaatcaac ccaataatgt cggtgggtga tagtgtgata
    23821 acaactctgg agccgtaata tgaaaataat ctctaaaatg ttagtcggtg cgttagcgtt
    23881 tgccgttacc aatgtctatg ccgctgaatt gatgaccaaa gcggaatttg aaaaagttga
    23941 atcgcagtat gaaaaaatag gtgatatttc aaccagtaat gaaatgtcga ctgcagatgc
    24001 aaaagaagat ttaatcaaaa aagccgatga aaaaggggct gatgtgttgg tactgacctc
    24061 cggtcaaact gacaataaga tccacggcac ggcggatatt tataagaaga agtaattctg
    24121 aatcctatgt aaacatcccc gatgcgtaag tttatcggtg atcatctatt gaaatttatg
    24181 ccggataaag cgttcgcgct gcattcggca gttcagcttt tcagccgccg ccagaacgtc
    24241 gtccggctga tgcctaaata attcgccgct gctgttttat cgccattaaa tttctccagt
    24301 gcctgttgtg gtgtcagcaa gcgtggagcg ggaattttcg ccgactcgcg cgccagttcc
    24361 ggcagtagca gctgcaaaaa ctgcggcgtt aaatccggcg tcggttccac acttaaaaat
    24421 agcgccagtc gctccatcat attgcgcagt tcacgaatat tacctggcca gtcgtagtgc
    24481 accagcacgg tttcgcttgc ctgtaatccc tggcgtaatg cggcagaaaa tggggcggag
    24541 agcgccgcca gagacacttt caaaaagctt tccgccagcg gaagaatatc tgccacccgc
    24601 tcgcgcagtg gtggcaattg cagacgcaaa atactcagcc gataaaacag atcacggcga
    24661 aactgccctt gccgcatatc ttcttccaga ttgcagtgag tggcgctaat gacccgtaca
    24721 tctaccggaa caggctgatg cccgccgacg cgggtgacct ctttttcttc cagcacccgt
    24781 aacaggcgag tctgcaaagg tagcggcatt tcgccaatct catccagaaa cagcgtgcca
    24841 ccgtgggcaa tttcgaacag cccggcgcga cctccgcgtc gcgagccggt aaacgcccct
    24901 tcctcatagc caaacagctc cgcttccagc agcgattcgg caatcgcccc gcagttgacg
    24961 gcaacaaacg gatgcgactt tttgccctgt cgcgcatcgt ggcgggcaaa atattcccga
    25021 tgaattgcct gggccgccag ctctttgccc gtccccgttt ccccctcaat caacaccgcc
    25081 gcactggagc gggcatacag caaaatagtc tgccgcacct gctccatctg tggtgattga
    25141 ccgagcatat cgcccagcac gtaacgagta cgcagggcgt tgcgggtggc atcgtgagtg
    25201 ttatggcgta acgacatgcg cgtcatatcc agcgcatcgc tgaacgcctg gcgcacggtg
    25261 gcggcggaat agataaaaat tccggtcatt ccggcttctt ctgccagatc ggtaatcagc
    25321 cccgcgccga ccaccgcttc ggtgccgtta gcttttagct cgttaatctg cccgcgtgcg
    25381 tcttcttcgg taatgtagct acgttgatcg aggcgcaaat taaaggtttt ttgaaacgcc
    25441 accagtgccg gaatagtttc ctgataagtg acaacgccga tagaagaggt gagttttccg
    25501 gcttttgcca gtgcctgtaa cacatcgtag ccgctcggtt taatcaaaat aactggcact
    25561 gacaggcggc ttttcaggta cgcgccgtta gagccagcgg cgatgatggc gtcacagcgt
    25621 tcgtttgcca gtttcttgcg gatgtaggtc actgcttttt caaagccaag ctgaataggg
    25681 gtaatgttcg ccaggtgatc aaactcgagg ctgatatcgc gaaacagctc gaataagcgc
    25741 gttacagata ccgtccagat aaccggtttg tcgtcattca gccgtggtgg atgtgccata
    25801 gcgcaccgca aagttaagat accgaatatt gggtttagtc ttgtttcata attgttgcaa
    25861 tgaaacgcgg tgaaacattg cctgaaacgt taactgaaac gcatatttgc ggattagttc
    25921 atgactttat ctctaacaaa ttgaaattaa agatttaatt ttattaaggc aattgtggca
    25981 caccccttgc tttgtcgtta tcaacgaaaa taacaagttg ataacaaagg atgggctatg
    26041 tctctacact ctccaggtaa agcgtttcgc gctgcactga ctaaagaaaa tccattgcag
    26101 attgttggca ccattaacgc caaccatgcg ctgttggcgc agcgtgccgg atatcaggca
    26161 atttatctct ccggcggtgg cgtggcggca gggtcgctgg ggctgcccga tctcggtatt
    26221 tctactcttg atgacgtgtt gacagatatt cgccgtatca ccgacgtttg ttcgctgccg
    26281 ctgctggtgg atgcggatat cggttttggt tcttctgctt ttaacgttgc gcgtacggtg
    26341 aaatcgatga ttaaagccgg tgcggcggga ttgcatattg aagatcaggt tggtgcaaaa
    26401 cgctgtggtc atcgcccgaa caaagcgatc gtctcgaaag aggagatggt ggatcggatc
    26461 cgcgcggcgg tggatgcgaa aaccgatcct gattttgtga tcatggcgcg caccgatgcg
    26521 ctggcggtag aggggctgga tgcggcgatc gagcgtgcgc aggcctatgt tgaagcgggt
    26581 gccgagatgt tgttcccgga ggcgattacc gaactcgcca tgtaccgcca gtttgccgat
    26641 gcggtgcagg tgccaatcct cgccaacatt accgaatttg gcgccacgcc gctgtttacc
    26701 accgacgaat tacgcagcgc tcatgtcgcg atggcgctgt acccgctttc agcgtttcgc
    26761 gccatgaacc gcgccgccga acatgtctac aacgtcctgc gccaggaagg cacgcagaaa
    26821 agcgtcatcg acaccatgca gacccgcaac gagctgtacg aaagcatcaa ctactaccac
    26881 tacgaagaga agctcgacga cctgtttgtc cgtagccagg cgaaataaaa aacgcccgtt
    26941 ggttgtattc gacaaccgat gcctgatgcg ccgctgacgc gacttatcag gcctacgggg
    27001 tgaactgtag gtcggataag acgcatggcg tcgcatccga caacaatctc gaccctacaa
    27061 atgataacaa tgacgaggac aatatgagcg acacaacgat cctgcaaaac agtacccatg
    27121 tcattaaacc gaaaaaatct gtggcgcttt ctggcgttcc ggcgggcaat acggcgctct
    27181 gcaccgtggg taaaagtggc aatgacttgc attaccgtgg ctacgatatt cttgatctgg
    27241 cggaacattg cgaatttgaa gaagtggcgc atctgctgat ccacggcaaa ctgccgaccc
    27301 gtgacgaact cgccgcctac aaaacgaaac tgaaagccct gcgcggttta ccggctaacg
    27361 tgcgtaccgt gctggaagcc ttaccggcgg cgtcacaccc gatggatgtt atgcgcaccg
    27421 gcgtttccgc gctcggctgc acgctgccag aaaaagaggg gcataccgtc tctggcgcgc
    27481 gggatattgc cgacaaactg ctggcgtcac ttagctcgat tcttctctac tggtatcact
    27541 acagccacaa cggcgaacgc atccagccgg aaaccgatga cgactccatc ggtggtcact
    27601 tcctgcatct gctgcacggc gaaaagccgt cgcaaagctg ggaaaaggcg atgcatattt
    27661 cgctggtgct gtacgccgaa cacgagttta acgcctccac ctttaccagc cgggtgattg
    27721 cgggcactgg ctctgatatg tattccgcga ttattggcgc gattggtgca ctgcgcgggc
    27781 cgaagcacgg cggggcgaat gaagtgtcgc tggagatcca gcaacgctac gaaacgccgg
    27841 acgaagccga agccgatatc cgcaagcggg tggaaagcaa agaagtggtc attggttttg
    27901 gtcatccggt ttataccatc gccgacccgc gccaccaggt gattaaacgt gtggcgaagc
    27961 agctctcgca ggaaggcggc tcgctgaaga tgtacaacat cgccgatcgc ctggaaacgg
    28021 tgatgtggga gagcaaaaag atgttcccca atctcgactg gttctccgct gtttcctaca
    28081 acatgatggg cgttcccacc gagatgttca caccactgtt tgttatcgcc cgcgtcaccg
    28141 gctgggcggc gcacattatc gaacaacgtc aggacaacaa aattatccgt ccttccgcca
    28201 attatgttgg accggaagac cgccagtttg tcgcgctgga taagcgccag taaacctcta
    28261 cgaataacaa taaggaaacg tacccaatgt cagctcaaat caacaacatc cgcccggaat
    28321 ttgatcgtga aatcgttgat atcgtcgatt acgtcatgaa ctacgaaatc agctccaaag
    28381 tagcctacga caccgcacat tactgtctgc tcgacacgct cggctgcggt cttgaagctc
    28441 tcgaataccc agcctgtaaa aaactgctgg ggccaattgt ccccggcacc gtcgtaccta
    28501 acggcgtgcg cgtccccgga actcagttcc agctcgaccc cgtccaggcg gcatttaaca
    28561 tcggcgcgat gatccgctgg ctcgatttca acgatacctg gcttgcggca gagtggggcc
    28621 atccttccga caatctcggc ggcattctgg caacggcgga ctggctttca cgcaacgcgg
    28681 tcgccagcgg caaagcgccg ttgaccatga aacaggtgct gaccggaatg attaaagccc
    28741 atgaaattca gggctgcatc gcgctggaaa actcctttaa ccgcgtcggc ctcgaccacg
    28801 ttctgttagt gaaagtggct tccaccgccg tggtcgccga aatgcttggc ctgacccgcg
    28861 aggaaattct caacgctgtt tcgctggcgt gggtggacgg tcagtcgctg cgcacctatc
    28921 gccatgcgcc gaacaccggc acgcgtaaat cctgggcggc gggcgatgcc acttcccgcg
    28981 cggtacgtct ggcactgatg gcgaaaacgg gcgaaatggg ttacccgtca gccctgactg
    29041 cgcctgtgtg gggcttctac gacgtctcct ttaaaggtga atcgttccgc ttccagcgtc
    29101 cgtacggttc ttacgtgatg gaaaatgtgc tgttcaaaat ctccttcccg gcggagttcc
    29161 actcccagac ggcagttgaa gcagcgatga cgctctatga acagatgcag gcagcaggca
    29221 aaacggcggc agatatcgaa aaagtgtcca tccgcaccca cgaagcctgt attcgcatca
    29281 tcgacaaaaa ggggccgctc aataacccgg cagaccgcga ccactgcatt cagtacatgg
    29341 tggcgatccc actgctattc gggcgcttaa cggcggcaga ttacgaggac aacgttgcgc
    29401 aagataaacg cattgacgcc ctgcgcgaga agatcaattg ctttgaagat ccggtattta
    29461 ccgctgacta ccacgacccg gaaaaacgcg ccatcgccaa tgccattacc cttgagttca
    29521 ccgacggcac acgatttgaa gaagtggtgg tggagtaccc cattggtcat gctcgccgcc
    29581 gtcaggatgg tattccgaaa ctggtcgata aattcaaaat caatctcgcg cgccagttcc
    29641 cgactcgcca acagcagcgc attctggagg tttctctcga cagagctcgc ctggaacaga
    29701 tgccggtcaa tgagtatctc gacctgtacg tcatttaagt aaacggcggt aaggcgtaag
    29761 ttcaacagga gagcattatg tcttttagcg aattttatca gcgttcgatt aacgaaccgg
    29821 agcagttctg ggccgagcag gcccggcgta ttgactggca gacgcccttt acgcaaacgc
    29881 tcgatcacag caatccgccg tttgcccgtt ggttttgtga aggccgaacc aacttgtgcc
    29941 acaacgctat cgaccgctgg ctggagaaac aaccagaggc gctggcgctg attgccgtct
    30001 cttcggaaac agaagaagag cgcaccttta cctttcgcca gcttcatgac gaagtaaacg
    30061 cggtggcgtc aatgctgcgc tcgctgggcg tgcagcgtgg cgatcgggtg ctggtgtata
    30121 tgccgatgat tgccgaagcg catattactc tgctggcctg cgcgcgcatt ggcgctattc
    30181 actcggtggt gtttggggga tttgcctcgc acagcgtggc ggcgcgaatt gatgatgcta
    30241 aaccggtgct gattgtctcg gctgatgccg gagcgcgcgg tggcaaaatc attccctata
    30301 aaaaattgct cgacgatgcg ataagtcagg cgcagcacca gccacgccat gttttgctgg
    30361 tggatcgcgg gctggcgaaa atggcacgcg tcagcgggcg ggatgtcgat ttcgcgtcgt
    30421 tgcgccatca acacatcggc gcgcgggtac cggtggcgtg gctggaatcc aacgaaacct
    30481 cctgcattct ctacacctcc ggcacgaccg gcaaacctaa aggcgtgcag cgtgacgttg
    30541 gcggatatgc ggtggcgctg gcgacctcga tggacaccat ttttggcggc aaagcgggcg
    30601 gtgtgttctt ttgtgcttcg gatatcggct gggtggtggg gcattcgtat atcgtctacg
    30661 cgccgctgct ggcggggatg gcgactatcg tttacgaagg attaccgacc tggccggact
    30721 gcggcgtgtg gtggaaaatc gtcgagaaat atcaggttag ccggatgttc tcagcgccga
    30781 ccgccattcg cgtgctgaaa aaattcccta ccgctgaaat tcgcaaacac gatctctcgt
    30841 cgctggaagt gctctatctg gctggagaac cgctggacga gccgaccgcc agttgggtga
    30901 gcaatacgct ggatgtgccg gtcatcgaca actactggca gaccgaatcc ggctggccga
    30961 ttatggcgat tgctcgcggt ctggacgaca ggccgacgcg tctgggaagc cccggcgtgc
    31021 cgatgtatgg ctataacgtg cagttgctta atgaagtcac cggcgaaccg tgtggcgtca
    31081 acgagaaagg gatgctggtg gtggaagggc cgctgccgcc ggggtgtatt cagaccatct
    31141 ggggcgacga cgaccgcttt gtgaagacgt actggtcgct gttttcccgc ccggtgtacg
    31201 ccacctttga ctggggcatc cgtgacgctg acggttatca ctttattctc gggcgcactg
    31261 acgatgtgat taacgttgcc gggcatcggc tggggacgcg cgagattgaa gagagtatct
    31321 ccagtcatcc gggcgttgcc gaagtggcgg tggttggggt gaaagatgcg ctgaaagggc
    31381 aggtggcggt ggcgtttgtc attccgaaag agagcgacag tctggaagat cgtgatgtgg
    31441 cgcattcgca agagaaggcg attatggcgc tggtggacag ccagattggc aactttggcc
    31501 gcccggcgca cgtctggttt gtctcgcaat tgccaaaaac gcgatccgga aaaatgctgc
    31561 gccgcacgat ccaggcgatt tgcgaaggac gcgatcctgg ggatctgacg accattgatg
    31621 atcctgcgtc gttgaatcag atccgccagg cgatggaaga gtaaggtatt gtcggatgcg
    31681 tcgcgtggcg catccgacag ttgtgcgcag atgcctgatg cgacgctggc gcgtcttatc
    31741 atgcctacag gcctgaaccg taggtcggat aaggcgctcg cgccgcatcc gacaccatgc
    31801 gcagatgcct gatgcgacgc tgatgcgtct tatcatgcct acaggcctga accgtaggtc
    31861 ggataaggcg ctcgcaccgc attcgacacc atgcgccgat gcctgatgcg acgctgatgc
    31921 gtcttatcat gcctacaggc ctgaaccgta ggtcggataa ggcgctcgcg ccgcattcga
    31981 caccatgcgc cgatgcctga tgcgacgcag gcgcgtctta tcatgcctac aggcctgaac
    32041 cgtaggtcgg ataaggcgct cgcgccgcat ccgacaccat gcgcagatgc ctgatgcgac
    32101 gctgatgcgt cttatcatgc ctacaggcct gaaccgtagg tcggataagg cgctcgcacc
    32161 gcatccgaca ccgtgctcag tgcctgatgc gacgctgatg cgtcttatca tgcctacagg
    32221 cctgaaccgt aggtcggata aggcgctcgc gccgcatccg acaccgtgcg cagatgcctg
    32281 atacgacgca ggcgcgtctt atcatgccta caggcctgaa ccgtaggtcg gataaggcgc
    32341 tcgcgccgca tccgacaccg tgcgtagttg cctgatgcga cgctgacgcg tcttatcatg
    32401 cctacccact gtttttacac cgataatttt ccccccacct ttttgcactc attcatataa
    32461 aaaatatatt tccccacgaa aacgattgct ttttatcttc agatgaatag aatgcggcgg
    32521 attttttggg tttcaaacag caaaaagggg gaatttcgtg tcgcaagata acaactttag
    32581 ccaggggccg gtcccgcagt cggcgcggaa aggggtattg gcattgacgt tcgtcatgct
    32641 gggattaacc ttcttttccg ccagtatgtg gaccggcggc actctcggaa ccggtcttag
    32701 ctatcatgat ttcttcctcg cagttctcat cggtaatctt ctcctcggta tttacacttc
    32761 atttctcggt tacattggcg caaaaaccgg cctgaccact catcttcttg ctcgcttctc
    32821 gtttggtgtt aaaggctcat ggctgccttc actgctgctg ggcggcaccc aggttggctg
    32881 gtttggcgtc ggtgtggcga tgtttgctat tccggtgggc aaggcaaccg ggctggatat
    32941 taatttgctg attgccgttt ccggtttact gatgaccgtc accgtcttct ttggcatttc
    33001 ggcgctgacg gttctttcgt tgattgcggt tccggctatc gcctgtcttg gcggttattc
    33061 cgtgtggctg gccgttaacg gcatgggcgg cctggacgcc ttaaaagcgg tcgttcccgc
    33121 acaaccgtta gatttcaatg tcgcgctggc gctggttgtg gggtcattta tcagtgcggg
    33181 cacgctcacc gctgactttg tccgctttgg tcgcaatgcc aaactggcgg tgctggtggc
    33241 gatggtggcc tttttcctcg gcaactcgtt gatgtttatt ttcggtgcag cgggcgctgc
    33301 ggcactgggg atggcggata tctctgatgt gatgattgct cagggcctgc tgttgcctgc
    33361 gattgtggtg ctggggctaa atatctggac caccaacgat aacgcgctct atgcgtcggg
    33421 tttaggtttc gccaacatta ccgggatgtc gagcaaaacc ctttcggtaa tcaacggtat
    33481 tatcggtacg gtctgtgcat tatggctgta taacaatttt gtcggctggt tgaccttcct
    33541 ttcggcagct attcctccag tgggtggcgt gatcattgcc gactatctga tgaaccgtcg
    33601 ccgctatgag cactttgcga ccacgcgtat gatgagtgtc aattgggtgg cgattctggc
    33661 ggtcgccctg gggattgctg caggccactg gttaccggga attgttccgg tcaacgcggt
    33721 attaggtggc gcgctgagct atctgatcct taacccgatt ttgaatcgta aaacgacagc
    33781 agcaatgacg catgtggagg ctaacagtgt cgaataacgc tttacaaaca attattaacg
    33841 cccggttgcc aggcaaagag gggctgtggc agattcatct gcaggacgga aaaatcagcg
    33901 ccattgatgc gcaatccggc gtgatgccca taactgaaaa cagcctggat gccgaacaag
    33961 gtttagttat accgccgttt gtggagccac atattcacct ggacaccacg caaaccgccg
    34021 gacaaccgaa ctggaatcag tccggcacgc tgtttgaagg cattgaacgc tgggccgagc
    34081 gcaaagcgtt attaacccat gacgatgtga aacaacgcgc atggcaaacg ctgaaatggc
    34141 agattgccaa cggcattcag catgtgcgta cccatgtcga tgtttcggat gcaacgctaa
    34201 ctgcgctgaa agcaatgctg gaagtgaagc tggaagtcgc gccgtggatt gatctgcaaa
    34261 tcgtcgcctt ccctcaggaa gggattttgt cgtatcccaa cggtgaagcg ttgctggaag
    34321 aggcgttacg cttaggggca gatgtagtgg gggcgattcc gcattttgaa tttacccgtg
    34381 aatacggcgt ggagtcgctg cataaaacct tcgccctggc gcaaaaatac gaccgtctca
    34441 tcgacgttca ctgtgatgag atcgatgacg agcagtcgcg ctttgtcgaa accgttgctg
    34501 ccctggcgca ccgtgaaggc atgggcgcgc gagtcaccgc cagccacacc acggcaatgc
    34561 actcttataa cggggcgtat acctcacgtc tgttccgctt gctgaaaatg tccggtatta
    34621 actttgtcgc caacccgctg gtcaatattc atctgcaagg acgattcgat acgtatccaa
    34681 aacgtcgcgg catcacgcgc gttaaagaga tgctggagtc cggcattaac gtctgctttg
    34741 gtcacgatga tgtcttcgat ccgtggtatc cgctgggaac ggcgaatatg ctgcaagtgc
    34801 tgcatatggg gctgcatgtt tgccagctga tgggctatgg gcagattaac gatggcctga
    34861 atttaatcac ccaccacagc gccaggacgt tgaatttgca ggattacagc attgccgccg
    34921 gaaacagcgc caacctgatt atcctgccgg ctgaaaatgg atttgatgcg ctgcgccgtc
    34981 aggttccggt acgttattcg gtacgtggcg gcaaggtgat tgccagcaca caaccggcac
    35041 aaaccaccgt atatctggag cagccggaag ccatcgatta caaacggtga tcggctgggc
    35101 tgtgtgttga caaagcgagc gtagtttatg ccggatgcgg cttgaacgcc ttatcaggcc
    35161 tacgtagagc actgaactcg taggcctgat aagcgtaacg catcaggcaa ttgtgtcagt
    35221 ggttaccgcg attcattctc gccaacatcc gcacattcct ccaacgccat gtgcaaaaat
    35281 gccttcgcgg cggctgtctg ccagctattt ttccgccgca acaaaaccgc cgttctctcc
    35341 agtagtggcg gggcaagagg aatcgcttta agcccgtcat gttgtgtggc aatcgctgct
    35401 ggtaacaatg tggaaaggga agtgcggcga atcagctcca gaaccgcgct aattgagttc
    35461 gcctcaatga ccacctgtgg atgcagcccc gctttctcgc agtagtggtc aatctgctct
    35521 ctggtggcaa attccgcgct gagcaggacc agtttttcat catgcaagcg actcaacgcc
    35581 acctgttcac aggcagccag cggatgatgt tgcgccacga ctaacgctaa actttctgtc
    35641 agtaaaggaa ttgcctccag ctccggcaaa tgcacaggcg cgaaggcaat cccaacgtcc
    35701 agctcgtcgc gacaaagcag atcttcgatt ttctcctgcg acatttcctg tagctggagc
    35761 gtgatgccgg ggtagcgcgc atagaaatcc gccattaagg ggccgataaa gtagctcgta
    35821 aaggtggggg tgacggcgat acgcagcgat cctctcgtca gatcggcaac atcatgaatc
    35881 gcccgtttac ccgctcccag ttcctgtaac gcccggctgg cgtactgtcg ccagacttct
    35941 cctgcatcag tgagatgaat cgttcgcccg ctacggtcaa acagcggcac gcctaaactc
    36001 tcctctaact ggcgaatctg ctgggaaagc gcaggctgag agacgtgcaa cgcactggcg
    36061 gcacgggtga agctgccatg ttcagccacg gcaagaaaat aattgatatg tcgagagagc
    36121 attcgccaac ctataagtaa attcaataga actcatcata aatgagactt ttaccttatg
    36181 acaatcggcg agtagtctgc ctctcattcc agagacagat agaggttaac ggtgaaagag
    36241 attattgatg gattccttaa attccagcgc gaggcatttc cgaagcggga agccttattt
    36301 aaacagttgg cgacacagca aagcccgcgc acacttttta tctcctgctc cgacagccgt
    36361 ctggtccctg agctggtgac gcaacgtgag cctggcgatc ttttcgttat tcgcaatgcg
    36421 ggcaatatcg tcccttccta cgggccggaa cccggtggcg tttctgcttc ggtggagtat
    36481 gccgtcgctg cgcttcgggt atctgacatt gtgatttgcg gtcattccaa ctgtggcgcg
    36541 atgaccgcca ttgccagctg tcagtgcatg gaccatatgc ctgccgtctc ccactggctg
    36601 cgttatgccg attcagcccg cgtcgttaat gaggcgcgcc cgcattccga tttaccgtca
    36661 aaagctgcgg cgatggtacg tgaaaacgtc attgctcagt tggctaattt gcaaactcat
    36721 ccatcggtgc gcctggcgct cgaagagggg cggatcgccc tgcacggctg ggtctacgac
    36781 attgaaagcg gcagcatcgc agcgtttgac ggcgcaaccc gccagtttgt gccactggca
    36841 gctaatcctc gcgtttgtgc cataccgcta cgccaaccga ccgcagcgta accttatttt
    36901 taaaccatca ggagatgcac catgattcag tcgcaaatta accgcaatat tcgtcttgat
    36961 cttgccgatg ccattttgct cagcaaagct aaaaaagatc tctcatttgc cgagattgcc
    37021 gacggcaccg ggctggcaga agcctttgtg accgcggctt tgctgggtca gcaggcgctt
    37081 cctgccgacg ccgcccgcca ggtcggggcg aagctggatc tcgacgaaga cgccattcta
    37141 ctgttacaga tgatcccact gcgtggctgc attgatgacc gtattccaac cgacccaacg
    37201 atgtatcgtt tctatgaaat gttgcaggtg tacggcacaa ccctgaaagc gttggttcat
    37261 gagaaatttg gcgatggcat tatcagcgcg attaacttca aactcgacgt taagaaagtg
    37321 gcggacccgg aaggtggcga acgtgcggtc atcaccttag atggtaaata tctgccgacc
    37381 aaaccgttct gacagccatg cgtaaccatc aaaagacgtt cacgatgctg ctggttctgg
    37441 tgctgattgg tcttaatatg cgaccactgc tcacctccgt cgggccactg ctaccgcaat
    37501 tgcgccaggc aagcggaatg agctttagcg tggctgccct gttgaccgct ctgccggtgg
    37561 ttaccatggg cgggctggcg ctggccggaa gctggcttca tcagcatgtc agcgaacgtc
    37621 gcagcgtcgc catcagtttg ttactgattg ccgtcggtgc attgatgcgt gagctttacc
    37681 ctcaaagtgc gctgctgctt agcagcgcac tgctcggtgg ggtggggatc ggcatcattc
    37741 aggcggtgat gccttcggtg attaaacggc ggtttcagca gcgcacgcca ctggtgatgg
    37801 ggctgtggtc cgcggctctg atgggcggcg gtgggcttgg tgccgccctg acaccctggt
    37861 tagttcaaca tagcgaaacc tggtatcaaa cactcgcctg gtgggcgctg cctgccgttg
    37921 ttgcgctctt tgcctggtgg tggcaaagca cccgcgaggt cgcctcttcc cacaagacaa
    37981 caaccattcc ggttcgcgtg gttttcactc ctcgcgcgtg gacgctgggt gtttacttcg
    38041 gtctgattaa cggtggttac gccagcctga ttgcctggtt acccgccttc tatattgaga
    38101 ttggtgccag cgcgcagtac agcggttcct tgctggcatt gatgacgctt gggcaagccg
    38161 caggagcttt gctgatgcct gctatggctc gccatcagga tcggcgcaaa ctgttaatgc
    38221 tggcgctggc gttacaactg gtgggtttct gcggctttat ctggctgccg ctgcaattgc
    38281 cggtattgtg ggcgatggtg tgtggtttag gtctgggcgg cgcatttccg ctctgtttgc
    38341 tgctggcgct cgatcactct gtgcaaccgg ctattgctgg caagctggtg gcgtttatgc
    38401 agggaatcgg ttttatcatc gccgggcttg ccccgtggtt ttctggcgtg ctgcgtagta
    38461 tcaggggcaa ttacttgatg gactgggcgt ttcatgcgct gtgcgtcgta gggctgatga
    38521 ttataaccct gcgttttgca ccagcacgtt ttccgcagct gtgggtcaaa gaggcatgat
    38581 gcgacgcttg tttctgcgct ttgttcacgc cggatgcggc ctacatagat cactgaattt
    38641 gtaggcctga taagcgaagc gtatcaggca atttttataa tttaaactga cgattcaact
    38701 ttgtaatctc tgaaataata ttgcttatcc cggtcgttta tttcgcgaat aacccgacaa
    38761 ggaacgccag ccgccacgac gtttggtgga atgtcttttg tgacgatact acccgcgcca
    38821 ataacagaat tatccccaat ggtgacgcct ggattaataa ccacatgact tccgatccag
    38881 acgttattgc caatcgttat cggaaaagag tacatctcac cgttttttct caattcatgg
    38941 tgtacagggt gtcccgtaac ggaaagagta acgttgggtg caatcaatac gttatcaccg
    39001 attgttaccg tgtagtcatc gacaatggtt aaattgaaat ttgcataaaa attgcggcct
    39061 atatggatgt tggaaccgta agagaaatag acggggggtt ctacccaggc gttttcccct
    39121 accgtggcaa acatttcttt aatcaggctt tctcttttat caacttctga tggatgcgag
    39181 tgattaaact catacattaa cgtttttcta cgaagtcttt tttcaggtaa gccttcgcac
    39241 atatcggtaa atagcttgcc tgcttttatt ctttctgtca tcgacatgtt cattgcgatc
    39301 attccgttat gatatgttgg tcggataagg cgctcgcgcc gcatccgaca ttgattgctt
    39361 aagcgacttc attcacctga cgacgcagta gagaaagcgg gccggggccg ctaagcgtga
    39421 acacggaaat taaggtgaag cccagcgcca ccagacccag caccagataa gcgccctgga
    39481 aaccgatgct ttcatacata ttacccgcta gtacagacat aaaaatcatc gccagttgct
    39541 taaagaagca gaaacagacc agataaatcg tcgctgaaaa acgcacttca aactggctgg
    39601 taatatattt aaagcagccc accagcagga acggtacttc aaacatatgc agcgttttca
    39661 gaataaccac ttccagcgct gaggtggcga acgatgagcc aataatacgt acagacataa
    39721 tagtgccagc cagcagcagg gcatttttcc caccgatgcg attaatgatc agtggcgcaa
    39781 agaacataat tgaggcgtta agtaattcgc ccattgtcgt tacgtagcca aatacccgcg
    39841 taccctgttc accggtggca aagaaagaag taaagaaatt agcaaactgt tggtcaaaaa
    39901 catcgtaggt gcaggaaacg ccaataacat acagtgacaa aaaccacagt tttggctgtc
    39961 tgaacagttc cagcgccagt ttaaggctaa atgccgaatg gttggcacct accgcattgg
    40021 caaccgtggc ggaagagggc gcatccgttt tggcgaaaaa gagtaaaatg gcgaggatga
    40081 gtgcacagcc agaacccagc cagaaaacga actgattatt gatggtgaac atgatgccga
    40141 caatcgaggc acacagcgcc cagccaacac agccaaacat ccgcgcgcga ccaaattcga
    40201 aattactgcg acggctgact ttctcgataa atgcctctac tgcgggcgca ccggcgttaa
    40261 aacaaaagcc aagataaata ccaccaacaa tcgatcctac taaaatgttg tattgtaaca
    40321 gtggcccgaa gataaaaata aagaacggcg caaacatcac taacatgccg gtaataatcc
    40381 acagcaggta tttgcgcagc ccgagtttgt cagaaagcag accaaacagc ggttggaata
    40441 atagcgagaa cagagaaata gcagcaaaaa taatacccgt atcacttttg ctgatatggt
    40501 tgatgtcatg tagccaaatc gggaaaaacg ggaagtaggc tcccatgata aaaaagtaaa
    40561 agaaaaagaa taaaccgaac atccaaaagt ttgtgttttt taaatagtac ataatggatt
    40621 tccttacacg aaatacgggc agacatggcc tgcccggtta ttattatttt tgacaccaga
    40681 ccaactggta atggtagcga ccggcgctca gctggaattc cgccgatact gacgggctcc
    40741 aggagtcgtc gccaccaatc cccatatgga aaccgtcgat attcagccat gttccttctt
    40801 ccgcgtgcag cagatggcga tggctggttt ccatcagttg ttgttggctg tagcggctga
    40861 tgttgaactg gaagtcgccg cgccactggt gtgggccata attcaattcg cgcgtcccgc
    40921 agcgcagacc gttttcgctc gggaagacgt acggggtata catgtctgac aatggcagat
    40981 cccagcggtc aaaacaggcg gcagtaaggc ggtcgggata gttttcttgc ggccctaatc
    41041 cgagccagtt tacccgctct gctacctgcg ccagctggca ggtcaggcca atccgtgccg
    41101 gatgcggtgt atcgctcgct acttcaacat caacggtaat cgccatttga ccactaccat
    41161 caatccggta ggttttccgg ctaataaata aggtttttcc ctgatgctgc catgcgtgga
    41221 cagtggtaat cagcaccgcg tcggcaagtg tatctgccgt gcactgcaac aacgccgctt
    41281 ctgcctggta atggcccgca gccttccagc gttcgaccca ggcgttaggg tcaatgcggg
    41341 tcgcttcact tacgccaatg tcgttatcca gcggtgcgcg ggtgaactga tcgcgcagcg
    41401 gcgtcagcag ttgtttttta tcgccaatcc acatctgtga aagaaagcct gactggcggt
    41461 taaattgcca acgcttatta tccagctcga tgcaaaaatc cgtttcgctg gtggtcagtt
    41521 gcgggatggc gtggggcgcg gaggggagtg tcacgctgag gttttccgcc agacgccact
    41581 gctgccaggc gctgatgtgt ccggcttctg accatgcggt cgcgttcggt tgcactacgt
    41641 gtaccgttag ccagagttgc ccggtgctct ccagtcgcgg tagttcaggc aattcaatta
    41701 cctgtttacc ttgcggagcg acatccagag gcacttcacc gcttgccagc ggcttaccat
    41761 ccagcgccac cgtccagtgc aggagctcgt tatcgctatg atggaacagg tattcgctgg
    41821 tcacttcgat ggttcgcccg gataaacgga actggaaaaa ctgctgctgg tgttttgctt
    41881 ccgtcagcgc cggatgcggc gtgcggtcgg caaagaccag accgttcatg cagaactggc
    41941 gatcattcgg cgtatcgcca aaatcaccgc cgtaagccga ccacggattg ccattttcat
    42001 catatttaat cagcgactga tccaccaagt cccagacgaa gccgccctgt aaacgggggt
    42061 actgacgaaa cgcctgccag tatttagcga agccgccaag actgttaccc atcgcgtggg
    42121 cgtattcgca aaggatcagt gggcgcattt ctccaggcag cgaaagccat tttttgatgg
    42181 accatttcgg cacagccggg aagggctggt cttcatccac gcgcgcgtac atcgggcaaa
    42241 taatatcggt tgcggaggtg tcggctccgc cgccttcata ctgcaccggg cgggatggat
    42301 cgacagattt aatccagcga tagagtgcgt cgtgattagc gccgtggcct gactcattcc
    42361 ccagcgacca gatgatcaca ctcgggtgat tacgatcgcg ctgcaccatt cgtgttactc
    42421 gttcgctcat ggccggtaac cagcgcggat catcggtcag acgattcatt ggcaccatgc
    42481 cgtgggtttc aatattggct tcatccacca catacaggcc gtagcggtcg cacagggtgt
    42541 accacagcgg atggttcgga taatgcgaac agcgcacggc gttaaagttg ttctgcttca
    42601 ttagcaggat atcctgcacc atcgtctgct catccatgac ctgaccatgc agaggatgat
    42661 gctcgtgacg gttaacgccg cgaatcagca acggcttgcc gttcagcagc agcagaccat
    42721 tttcaatccg cacctcgcgg aaaccgacat cgcaggcttc tgcttcaatc agcgtgccgt
    42781 cggcggtgtg cagttcaacc accgcacgat agatattcgg gatttcggcg ctccacagtt
    42841 tcgggttttc gacgttcaga cctagtgtga cgcgatcggc ataaccgcca cgctcatcga
    42901 taatttcacc gccgaaaggc gcggtgccgc tggcgacctg cgtttcaccc tgccacaaag
    42961 aaaccgtcac ccgcagctca tcgcgcagct cgccgtacat ctgaacttct gcctccagta
    43021 ccgcgcggct aaaatcatca ttaaagagag tggcaacatg gaaatcgctg atttgtgtgg
    43081 tcggtttgtg cagcaacgag acgtcacgga aaatgccgct catccgccac atatcctgat
    43141 cttccagata actgccgtca ctccagcgca gcaccatcac cgcgaggcgg ttttctccgg
    43201 cgcgtaaaaa tgcgctcagg tcaaattcag acagcaaacg actgtcctgg ccgtaaccga
    43261 cccagcgccc gttgcaccac agatgaaacg ccgagttaac gccatcaaaa ataattcgcg
    43321 tctggccttc ctgtagccag ctttcatcaa cattaaatgt gagcgagtaa caacccgtcg
    43381 gattctccgt gggaacaaat ggcggattga ccgtaatggg atatgtcacg ttggtgtaga
    43441 tgggcgcgtc gtaaccgtgc atctgccagt ttgaggggac gacgacagta tcggcgtcag
    43501 gaagatcgca ctccagccag ctttccggaa ccgcttctgg tgccggaaac cagacaaatt
    43561 gccactcacc attcaggctg cgcaactgct gggaagggcg attggtgcgg gcctcttcgc
    43621 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    43681 ggttttccca gtcacgacgt tgtaatacga cggccagtga atctgtaatc atagtcatag
    43741 ctgtatcctg tgtgaaattg ttatccgctc acaatttcac acaacatacg agccggacgc
    43801 atagagtgta aagcgtgggg tgcctaatga gtgagctgac tcacattaat tgcgttgcgc
    43861 tcactgcccg ctttccagtc gggaaacttg tcgtgccagc tgcattaatg aatcggccaa
    43921 cgcgcgggga gaagcggttt gcgtcttggg cgccagggtg gtttttcttt tcaccagtga
    43981 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
    44041 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
    44101 acacgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcaa
    44161 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
    44221 cgcagtggga acgatgccct catttagcat ttgcatggtt tgttgaaaac cggacatggc
    44281 actccagtcg ccctcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
    44341 ccagcccgcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
    44401 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
    44461 gaaaataata ctgttgatgg gagtctggtc agagacatca agaaataacg ccggaacatt
    44521 agcgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
    44581 cccactgacg cgttgcgcga gaagattgtg cactgccgct ttacaggctt cgacgccgct
    44641 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
    44701 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
    44761 cgactgtttc cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
    44821 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctgat tcaccacgcg
    44881 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtatagcg ttactggttt
    44941 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
    45001 tttgcgccat tcggcggggc tgacgccgaa gtgagattta aaatgctgac gccaggtcac
    45061 aggtgagaga aaacccacct cagccgctat ccgctcaacg ggcaactgac tggatcccaa
    45121 cagttcctgg ctacggcgta agcgttcagt aatgagccat tcggcgatac tcgaaccggt
    45181 cgctttcata aaatggcgag ttaatgtgcg gcgactcatc attactcgct gcgccagcga
    45241 gtcgagatca tgctgttgcg caatgttttg ccgcaggtaa tccagcagga ggtttatgcg
    45301 ttcatcatgg gtatttttcg gcaccggttg ctcaataaac tgcgcctgac cgccggtgcg
    45361 atatggcggt acgaccatcc gtcgggcaat atggttagca tagtcgctgc caaaatgttg
    45421 ccgaacaata tacaaacagc aatccagcgc cgcggcagta ccggcagagg taataatgcc
    45481 gtcatcgtct acgtacaacg cgttaatatc cagatgtgtt tgtggaaaac gtgcctgaaa
    45541 ttcacgctcg aactcccagt gcgtggcggc acgcttattt ttcagtaaac ctgcatagcc
    45601 gagcacaaac gtgcccaggc aaagcccgac aatctgtgcg ccgttatccc gtgcggtcgt
    45661 taaggcttcc agcagttttt gtggcggttt ttgtgtaatg gttccccagt aaggaattat
    45721 cacaatatct gcctggatga ctgcggtata gtcatgggtg gcggttacgg aaaacccatc
    45781 ggcagagtcc acgtttcccg gcttttcagc gcaaataatc acgtgaaagc gttttttctc
    45841 cgccatcttt tcactaaaca cgataaacgg tacggaaagg tgaaaaggac taaaaccggc
    45901 aacggcgata atggcaacag ttagcgggcg cataacactc tttatctccg aattagaatg
    45961 tcaacgtttc accatcttct ggtacgttca caaattcgat aatttgatta tctctggcgt
    46021 attccttgag cgcatcacgc gtcagcaaac agtggtttac ggcctccata tgtgtcgcga
    46081 taatttgcgc ttctgggagg gtaaaatgtg ttttgagaat atcttctgct cccataatta
    46141 tcgcgccatg ccccagaaca tgcgcaaatc cggcattcaa cacaaccact tctggttgga
    46201 aagttagcat tgtagcctcg acgtcgtcac gccagagggt atcgccagcc agatagagcg
    46261 ttttctctgt cgggtgctgg aaaacaacgc cgcaagctgc gcctaaacgc ttcgccatcg
    46321 ccgggtcggc ataggtgcga tcggagccgt gctgccctgt cgttttatgc agagtaatat
    46381 cgccaaattt agtctcgtcg gtaagtacgg taagatgcgt aaatccttgt ttattcagtg
    46441 attcagcatc ttgctcattt tgcacataaa tcagtttatc tttagggacg acgcgggagg
    46501 ctgtttcgtc ccagtgatct tcatgcaaat gggtcacgat tagggcgtca acgttgagta
    46561 acgttgttaa atcgcacggt aattctaccg ttggattgcg gatttctgcg cgtgccgttc
    46621 cggcaaaacc cggataactt cctttaggcg cgagtaacgg gtcgataaga aatgttttgc
    46681 cagcgtattg gataatttgg gtggcattac gaatttgcgt aattttcatg atttgctcca
    46741 catcacagta ttgatgtcat tagcgtagcg tgcagcccgt actgtgcatg tgggcaaatg
    46801 ggcgaataac gatgagaatg ggccaaatta cacattggcg taactagcgt aaatgcacgc
    46861 cgcttcgccg tccggccacc agaatagcct gcgattcaac cccttcttcg atctgttttg
    46921 ctacccgttg tagtgccgga agatgctttt ccgctgcctg ttcgatggtc attgcgctcg
    46981 ccatatacac cagattcaga cagccaatca cccgttgttc actgcgcagc ggtacggcga
    47041 tagaggcgat cttctcctcc tgatcccagc cgcggtagtt ctgtccgtaa ccctctttgc
    47101 gcgcgcgcgc cagaatggct tccagcttta acggttctcg tgccagttga tagtcatcac
    47161 cggggcggga ggctaacatc tcgattaatt ccttgcgttc ttgttccggg caaaaggcca
    47221 gccaggtcag gcccgaggcg gttttcagaa gcggtaaacg ccgcccgacc attgcccggt
    47281 gaaaggataa gcggctgaaa cggtgagtgg tttcgcgtac caccattgca tcaacatcca
    47341 gcgtggacac atctgtcggc cataccactt cgcgcaacag atcgcccagc agtggggccg
    47401 ccagtgcaga aatccactgt tcgtcacgaa atccttcgct taattgccgc actttgatgg
    47461 tcagacgaaa actatcatcg gagggactac gccggacata tccctcttcc tgcagcgtct
    47521 ccagcagtcg ccgcacagtg gtgcgatgca ggccgctgag ttccgccagc agcccgacgc
    47581 tggcaccgcc gtcaagttta tttaacatat ttaataacat tagaccgcgg gttaagccgc
    47641 gcacggtttt gtattccgtc tgctcattgt tctgcatatt aattgacatt tctatagtta
    47701 aaacaacgtt gtgcacctgg tgcacattcg ggcatgtttt gattgtagcc gaaaacaccc
    47761 tttctatact gagcgcacaa taaaaaatca tttacatatt tttaacaaaa taagttgcgc
    47821 tgtactgtgc gcgcaacggc attttgtccg agtcgtgagg tactgaaatg gcaatacaac
    47881 accctgacat ccagcctgct gttaaccata gcgttcaggt ggcgatcgct ggtgccggtc
    47941 cggtcgggct gatgatggcg aactatctcg gccagatggg cattgacgtg ctggtggtgg
    48001 agaaactcga taagttgatc gactacccgc gtgcgattgg tattgatgac gaggcgctgc
    48061 gcaccatgca gtcggtcggc ctggtcgata atgttctgcc gcatactacg ccgtggcacg
    48121 cgatgcgttt tctcaccccg aagggccgct gttttgctga tattcagcca atgaccgatg
    48181 aatttggctg gccgcgccgt aacgccttta ttcagccgca ggtcgatgcg gtgatgctgg
    48241 aaggggtgtc gcgttttccg aatgtgcgct gcttgttttc ccgcgagctg gaggccttca
    48301 gtcagcaaga tgacgaagtg accttgcacc tgaaaacggc agaagggctg cgggaaatag
    48361 tcaaagccca gtggctggtg gcctgtgatg gtggggcaag ttttgtccgt cgcaccctga
    48421 atgtgccgtt tgaaggtaaa actgcgccaa atcagtggat tgtggtagat atcgccaacg
    48481 atccgttaag tacgccgcat atctatttgt gttgcgatcc ggtgcgcccg tatgtttctg
    48541 ccgcgctacc tcatgcggta cgtcgctttg aatttatggt gatgccggga gaaaccgaag
    48601 aacagctgcg tgagccgcaa aatatgcgca agctgttaag caaagtgctg cctaatccgg
    48661 acaatgttga attgattcgc cagcgtgtct acacccacaa cgcgcgactg gcgcaacgtt
    48721 tccgtattga tcgcgtactg ctggcgggcg atgccgcgca catcatgccg gtatggcagg
    48781 ggcagggcta taacagtggt atgcgcgacg cctttaacct cgcatggaaa ctggcgttgg
    48841 ttatccaggg gaaagcccgc gatgcgctgc tcgataccta tcaacaagaa cgtcgcgatc
    48901 acgccaaagc gatgattgac ctgtccgtga cggcgggcaa cgtgctggct ccgccgaaac
    48961 gctggcaggg tacgttacgt gacggcgttt cctggctgct gaattatctg ccgccagtaa
    49021 aacgctactt cctcgaaatg cgcttcaagc cgatgccgca atattacggc ggtgcgctgg
    49081 tgcgtgaggg cgaagcgaag cactctccgg tcggcaagat gtttattcag ccgaaagtca
    49141 cgctggaaaa cggcgacgtg acgctgctcg ataacgcgat cggcgcgaac ttcgcggtaa
    49201 ttggctgggg atgcaatcca ctgtggggga tgagcgacga gcaaatccag cagtggcgcg
    49261 cgttgagcac acgcttcatt caggtggtgc cggaagtgca aattcatacc gcacaggata
    49321 accacgacgg cgtactacgc gtgggggata cgcaaggtcg cctgcgtagc tggttcgcgc
    49381 aacacaatgc ttcgctggtg gtgatgcgcc cggatcgctt tgttgccgcc accgccattc
    49441 cgcaaacact gggcaagacc ctgaataaac tggcgtcggt gatgacgctg acccgccctg
    49501 atgccgacgt ttctgtcgaa aaggtagcct gatatgcacg cttatcttca ttgtctttcc
    49561 cactcgccgc tggtggggta tgtcgacccg gcacaagagg tactcgatga ggtcaatggc
    49621 gtgattgcca gcgcccgcga gcgtattgcg gctttctccc ctgaactggt ggtgctgttt
    49681 gcgccagatc actacaacgg ctttttctat gacgtgatgc caccgttctg tttaggcgtt
    49741 ggagcgacgg caattggtga tttcggcagt gcggcaggag agctgcccgt gcctgtggag
    49801 ctggcggagg cctgtgcgca tgccgtcatg aagagcggga tcgatcttgc cgtttcttac
    49861 tgtatgcagg tggaccacgg gttcgcccag ccgctggagt tcctgctcgg tgggctggat
    49921 aaggtgccag ttctgcctgt gttcatcaac ggtgtcgcca cgccgctgcc cggtttccag
    49981 cgtacccgca tgttgggtga agccattgga cgtttcacca gcactctcaa taaacgcgtg
    50041 ctgttcctag gttccggtgg gctttcccat cagccgccgg tgcccgaact ggcgaaagcc
    50101 gatgcccata tgcgcgaccg cctgttgggg agcgggaaag atttacccgc cagtgagcgc
    50161 gaattgcgtc agcaacgggt gattagcgcc gctgagaagt ttgttgagga tcagagaact
    50221 ctgcatccgc tcaacccgat ttgggataac cagttcatga ctttgctgga gcagggacgc
    50281 atacaggaac tggatgccgt cagtaacgaa gagctttccg ccattgccgg aaagtcgaca
    50341 catgaaatca aaacctgggt cgccgctttt gccgctattt ctgcatttgg caactggcgt
    50401 agcgaagggc gttattaccg cccaatcccg gagtggattg ccggatttgg ctcgttaagc
    50461 gccagaacag agaactgaat atgcaggaga agatgatgag ttatcagcca caaaccgaag
    50521 ccgccaccag ccgttttctg aatgtagaag aagcgggtaa aacgctgcgc atccatttta
    50581 atgactgcgg acaaggcgac gaaaccgttg tcctgctgca tggttccggc ccgggtgcta
    50641 ctggctgggc gaacttcagc cgcaatatcg atccgctggt agaggcgggc tatcgggtga
    50701 tcctgctgga ttgtccgggt tggggcaaga gcgattcgat cgttaatagt ggttcgcgat
    50761 cggatcttaa tgcacgaatc ctgaaaagcg tggtggatca actggatatc gccaaaatcc
    50821 acctgctggg caactcgatg ggtggccata gttctgtggc gttcaccctt aactggccgg
    50881 agcgcgtcgg caaactggtg ctgatgggcg gcggtacggg cggcatgagt ttgtttacgc
    50941 cgatgccaac cgaaggtatt aagcgactga atcagcttta tcgtcagccg actatcgaaa
    51001 acctgaagct gatgatggat atcttcgttt ttgataccag cgatttgacc gacgccctgt
    51061 ttgaagcgcg cctgaataat atgctgtcgc gccgcgatca cctggaaaac ttcgttaaga
    51121 gcctggaagc taatccgaaa cagttcccgg attttggccc acgtctggcg gaaatcaaag
    51181 cgcaaaccct gattgtctgg gggcgcaacg accgctttgt gccgatggat gcgggtctgc
    51241 gtctgctgtc cggcattgcc ggttctgaac tgcatatctt ccgcgactgt gggcattggg
    51301 cgcagtggga acatgccgac gctttcaatc aactggtgct gaatttcctc gcacgtgctt
    51361 aaggaacggt catgacgaag catactcttg agcaactggc ggcggattta cgccgcgccg
    51421 cagagcaggg cgaagcgatt gcgccgttgc gcgatctgat tggtatcgat aacgctgaag
    51481 cggcctacgc cattcagcac ataaatgtgc aatatgacgt tgcgcagggg cgtcgcgtgg
    51541 tggggcgtaa agtgggcctg acacatccga aagtgcagca acaactgggc gttgatcaac
    51601 cggatttcgg gacattattt gccgacatgt gttatggcga taacgaaatc attccttttt
    51661 cccgtgttct gcaaccccgc attgaagcgg agatcgcact ggtgttgaac cgcgatttac
    51721 ccgcaactga tatcaccttc gacgaattgt ataacgccat tgaatgggta cttccggcgc
    51781 tggaggtggt gggtagccgc attcgcgact ggtcgattca gtttgtcgat accgtggcag
    51841 ataacgcttc ctgcggggtg tatgtcatcg gcggtccggc gcaacgtccg gcggggttag
    51901 acctgaaaaa ctgcgccatg aagatgacgc gtaataacga agaggtttcc agcgggcgcg
    51961 gcagcgaatg cctgggacat ccgcttaatg ccgccgtctg gctggcacgc aaaatggcca
    52021 gtctgggtga accgctgcgc gccggagata tcattcttac cggggcatta ggtccgatgg
    52081 tggcagtgaa tgcgggcgat cgttttgaag cccatattga aggcataggt tcagttgctg
    52141 cggcattttc aagcgcagcc ccaaaaggaa gtctgtcatg agtaagcgta aagtcgccat
    52201 tatcggttct ggcaacattg gcaccgatct gatgattaaa attttgcgtc acgatcagca
    52261 tctggagatg gcggtgatgg tcggcattga tcctcagtcc gacggtctgg cgcgtgccag
    52321 acgtatgggc gtcgccacca cccatgaagg ggtgatcggg ctgatgaaca tgcctgaatt
    52381 tgctgatatc gacattgtat ttgatgcgac cagcgccggt gctcatgtga aaaacgatgc
    52441 ggctttgcgc gaagcgaaac cggatattcg cttaattgac ctgacgcctg ctgccatcgg
    52501 cccttactgc gtgccggtgg ttaacctcga ggagaacgtc gatcaactga acgtcaacat
    52561 ggtcacctgc ggcggccagg ccaccattcc aatggtggcg gcagtttcac gcgtggtgcg
    52621 tgttcattac gccgaaatta tcgcttctat cgccagtaaa tctgccggac ctggcacgcg
    52681 tgccaatatc gatgaattta cggaaaccac ttcgcgagcc attgaagtgg tgggcggcgc
    52741 ggcaaaaggg aaggcgatta tcgtgcttaa cccagcagag ccaccgttga tgatgcgtga
    52801 cacggtgtat gtattgagcg acgaagcttc acaagatgat atcgaagcct caatcaatga
    52861 aatggctgag gcggtgcagg cttacgtacc gggatatcgc ctgaaacagc gcgtgcagtt
    52921 tgaagttatc ccgcaggata aaccggttaa tttgccgggc gtagggcagt tctccgggct
    52981 gaaaacggcg gtctggctgg aagtcgaagg cgcagcgcat tatctgcctg cctatgcggg
    53041 caacctcgac attatgactt ccagtgcgct ggcgacagca gaaaaaatgg cccagtcact
    53101 ggcgcgcaag gcaggagaag cggcatgaac ggtaaaaaac tttatatctc ggacgtcacg
    53161 ttgcgtgacg gtatgcacgc cattcgtcat cggtattcgc tggaaaacgt tcgccagatt
    53221 gccaaagcac tggacgatgc ccgcgtggat tcgattgaag tggcccacgg cgacggtttg
    53281 caggggtcca gctttaacta tggtttcggc gcacatagcg accttgaatg gattgaagca
    53341 gcggcggatg tggtgaagca cgccaaaatc gcgacgttgt tgctgccagg aatcggcact
    53401 attcacgatc tgaaaaatgc ctggcaggct ggcgcgcggg tggttcgtgt ggctacgcac
    53461 tgtaccgaag ctgatgtttc cgcccagcat attcagtatg cccgcgagct cggaatggac
    53521 accgttggtt ttctgatgat gagccatatg accacgccgg agaatctcgc aaagcaggca
    53581 aagctgatgg aaggctacgg tgcgacctgt atttatgtgg tggattctgg cggtgcgatg
    53641 aacatgagcg atatccgtga ccgtttccgc gccctgaaag cagtgctgaa accagaaacg
    53701 caaaccggca tgcacgctca ccataacctg agtcttggcg tggcgaactc tatcgcggcg
    53761 gtggaagagg gctgcgaccg aatcgacgcc agcctcgcgg gaatgggagc gggcgcaggt
    53821 aacgcgccgc tggaagtatt tattgccgcc gtggataaac tgggttggca gcatggggcc
    53881 gatctctatg cgttaatgga tgctgctgac gacctggtgc gtccgttgca ggatcgaccg
    53941 gtacgagtcg atcgcgaaac gctggcgctg ggatacgctg gtgtttactc cagcttcctg
    54001 cgtcactgtg aaacggcggc ggcgcgttat ggcttaagcg cggtggatat tctcgttgag
    54061 ctgggcaaac gccggatggt tggcggccag gaggatatga tcgtcgacgt ggcgctggat
    54121 ctgcgcaaca acaaataata ttgactgccg agaacgtgca ttttgtatgc ccatcccagc
    54181 acgactgccg gatgcggcgt gaacgcctta tccggtctac atttcgcccg taggcagtca
    54241 ttaaatagtt ctgattacgc ggctggcaat gtatcagtcg cgattcacct gcacacgcaa
    54301 tgaggttcat cttatgtcga ctcgtacccc ttcatcatct tcatcccgcc tgatgctgac
    54361 catcgggctt tgttttttgg tcgctctgat ggaagggctg gatcttcagg cggctggcat
    54421 tgcggcgggt ggcatcgccc aggctttcgc actcgataaa atgcaaatgg gctggatatt
    54481 cagcgccgga atactcggtt tactacccgg cgcgttggtt ggcggaatgc tggcggaccg
    54541 ttatggtcgc aagcgcattt tgattggctc agttgcgctg tttggtttgt tctcgctggc
    54601 aacggcgatt gcctgggatt tcccctcact ggtctttgcg cggctgatga ccggtgtcgg
    54661 gctgggggcg gcgttgccga atcttatcgc cctgacgtct gaagccgcgg gtccacgttt
    54721 tcgtgggacg gcagtgagcc tgatgtattg cggtgttccc attggcgcgg cgctggcggc
    54781 gacactgggt ttcgcggggg caaacttagc atggcaaacg gtgttttggg taggtggtgt
    54841 ggtgccgttg attctggtgc cgctattaat gcgctggctg ccggagtcgg cggttttcgc
    54901 tggcgaaaaa caggctgcgc caccactgcg tgcgttattt gcgccagaaa cggcaaccgc
    54961 gacgctgctg ctgtggctgt gttatttctt cactctgctg gtggtttaca tgttgatcaa
    55021 ctggctaccg ctgcttttgg tggagcaagg attccagcca tcgcaggcgg caggggtgat
    55081 gtttgcactg caaatgggtg cggcgagcgg gacgttaatg ttgggcgcat tgatggataa
    55141 gctgcgtcca gtaaccatgt cgctactgat ttatagcggc atgttagctt cgctgctggc
    55201 gcttggaacg gtgtcgtcat ttaacggtat gttgctggcg ggatttgtcg cggggttgtt
    55261 tgcgacaggt gggcaaagcg ttttgtatgc cctggcaccg ttgttttaca gttcgcagat
    55321 ccgcgcaaca ggtgtgggaa cagccgtggc ggtagggcgt ctgggggcta tgagcggtcc
    55381 gttactggcc gggaaaatgc tggcattagg cactggcacg gtcggcgtaa tggccgcttc
    55441 tgcaccgggt attcttgttg ctgggttggc ggtgtttatt ttgatgagtc ggagattaag
    55501 aatgcagccg tgtgccgatg cctgacgcgc cttattaagc aggggcgaaa gggacttgcc
    55561 cccaatacac ccacggcgtt tacaggtata ctcgctaaaa attattcagc gggtttggaa
    55621 acaaagatgg caaaacttac cttacaagag cagttgctca aagcaggatt agtcaccagc
    55681 aaaaaagcgg cgaaggtcga gagaacggcg aaaaaatcgc gcgttcaggc gcgtgaagct
    55741 cgggcggcgg tagaagaaaa taaaaaggca cagcttgagc gtgataaaca gcttagcgaa
    55801 cagcaaaaac aagcggcgtt ggcgaaagaa tataaagctc aggtgaagca gcttattgaa
    55861 atgaaccgga tcaccattgc caatggcgat attggtttta acttcactga tggcaatctg
    55921 attaagaaga tttttgtcga taagctcaca caggcgcagt tgattaatgg tcgtctggcg
    55981 attgcccgct tgttggtcga taacaatagc gaaggtgaat acgccattat tcccgccagc
    56041 gttgccgata aaattgctca gcgtgatgcc agcagtattg tgttgcacag cgcgctcagt
    56101 gccgaagagc aggatgaaga cgacccgtat gccgacttca aagtgcctga tgatttgatg
    56161 tggtaaacat agataagtcg tgcgcagatg cctgatgcga cgctatgcgc gtcttatcat
    56221 gcctacggcc tgcgggcgaa atgtaggcgg ataaggcgtt cacgccgcat ccggcagtcg
    56281 tgcactatta tcaacgcata ttcagtttat tggcgtggta ggcaatatgc tcgccaataa
    56341 aactggagac aaaataatag ctgtggtcgt aaccctcgtg ataacggatt aacgttttga
    56401 tattcatctc ctggcagatc ttttcaagat tttgagtccg cagctgttcc gcgtaaaaat
    56461 catcactcaa cccctgatca accatgattt ccgcaacgcg ttgaccttgt gaaataagac
    56521 tcaccggatg gtaatccaac caggcctctt tattttcacc aagatatgcg gcaaaggctt
    56581 gctgtcccca cggtacttgc gatggggaga caatgggcga aaacgccgag acgctgacat
    56641 attcatctgg gttacgtaac gccagcacca gcgcgcccag tccgcccata gaatgaccag
    56701 agatagactt tttggccgtt gccggaaaat gattcatcac taaatccggc agctcgttgc
    56761 ggatatagtc atacattttg taatgttcat tccacggcgc ttgcgtcgcg ttcaggtaaa
    56821 acccggcacc ttgcccgaga tcgtaacggt cggcatctgc gacatgactg cctcgcggac
    56881 tggtgtccgg cgcaacaaca ataatgttgt gctcagccgc gtaacgctgc acccccgatt
    56941 tagtaatgaa attctgctca ttgcaggtca ggcccgaaag ccagtacagc accggtaatt
    57001 tttcacctgc agcttttggt gggagataga cgccgacatt catttcacat ttcagtgatg
    57061 cggaataatg ccgatacaca ttttgccagc cgccaaagct ggcatgtttt tcaatgagtt
    57121 ccatcagtca ttatctcacg gttaatcccc cggacggctg cccggagaga gtattaagaa
    57181 ccccgtctga agtggacggg gtaaaccagc gggaaatcag taacgaatta cggttcgaat
    57241 ggatttgcct tcatgcatca ggtcgaaggc gtcattaatt tcatccaggc tcatggtatg
    57301 cgtgacaaac ggttccagat cgatatcacc tttcatcgca tcttcaacca tacccggtaa
    57361 ctgggaacga cctttcacgc cgccaaacgc ggaacctttc catacgcgac cagtgaccaa
    57421 ctggaatgga cgggtggaga tttcctgacc ggaacccgcg accccgatga tcaccgactg
    57481 accccagccg cggtgcgcac tttccagcgc cgcacgcatc acgttgacat taccgatgca
    57541 ttcaaaggta tggtcgatac cccatttgtt gatatccagc aggacatctt taatcggttt
    57601 gtcgtagtca ttcgggttaa tgcagtcggt ggcaccgaag cgacgcgcca gatcgaattt
    57661 cttcgggttg gtatcgatag cgataatccg tcccgctttc gcctgacgcg cgccctgaac
    57721 cactgccaga ccaatcgcgc caagaccaaa cacagcaaca gaatcccctg gctggacttt
    57781 agctgtgttg tgtaccgcgc caataccggt ggtcacgcca cagcccagca ggcagacgtg
    57841 ttcatggttt gcttctggat taatttttgc cagagacact tccgcgacta cggtgtattc
    57901 actgaatgta gaacacccca tgtagtgata aagcggctgc ccattgtaag aaaaacgggt
    57961 ggtgccgtct ggcatcaggc ctttaccctg ggtttcgcga accgcaacac agaggttagt
    58021 tttgccagaa cgacagaact cacactcgcc gcactctgcg gtgtaaagcg ggatcacatg
    58081 gtcgcctggt ttgacgctgg ttacgccttc accgacttca accacaacgc cagccccttc
    58141 gtgaccgaga accaccggga atacaccttc cgggtcatcg ccggagaggg taaatgcgtc
    58201 ggtatggcaa acgccggtat gggtgacttt aatcagcact tcaccttttt tcggtggtgc
    58261 aacgtcaatt tcaacgattt ccagcggttt accgggagca aatgcaacgg cagcacgtga
    58321 tttcatctct cgctcttcct caatatggta atagattcag ctatttaaga taggcacgaa
    58381 ccagttcaat agtgtcgtca acggattggc tgacttcgcg gctgtagcag tcatttcggt
    58441 caaacgtttc ccggatatgg ctttcaagca cttctgccat cagcccatta gccgcgcccc
    58501 gaacggcagc gatctgttgg agtatggcac ggcattcggc atcaccctcc agcgaccgtt
    58561 ccagagcatc aatctgccct cgaatacgac gaactcgagt aaggaccttt ttcttctctt
    58621 ccggagtact gggcatttcg cacctcatca tctgcacgca atatactata ggggggtatg
    58681 ctatatgtca atgcataccc ccctatagta tataacaatg gagaataatt gttgtccgaa
    58741 gcctaaccag gtgaacggtc cctcaagaca gatggggacc gtttatgtgc tatttttatg
    58801 ccgcatccgc taaaacaaac atcccgtaag gatggatttc gaggtaatag tgctcaccaa
    58861 tatcgggctg taaacgggtg gcgtttactt gtaataaaat ctcctgtcca tgccactcta
    58921 cggtcacttc gtactgcggc cccatgtagg cgacatggcg aataacacaa cgctgactct
    58981 cttcgccgtg attgcttaag gtgatggctt ccgggcgaac gccgacggtg ccgctacctt
    59041 gcaccgggaa atgcgccgcg cgcggcaggc gataaccgta gatatcgaca tactcttcgc
    59101 taaagttcgc cgggaacagg ttggcgtcgc ccataaaact tgccataaag cgggaggctg
    59161 gctgacgata gagatcctgc ggtgagccaa tctgcatgat gtgaccttta ttcatcacca
    59221 gcacagtgtc ggaaaccgcg aaggcctcac tttgatcgtg agtcacgtag agcgacgtga
    59281 tattaaactg cttttgcagc tcgcggatct tatcgcgcat gctgcggcgc aggttggcat
    59341 cgaggttact taacggctca tcaaacagca gcacctttgg tttgaggatc aacgcgcggg
    59401 ccagcgccac gcgctgttgc tgaccaccgg aaatctggtc gacatagcgg tcctcgaacc
    59461 cttccagatc caccattgcc agcgcctctt tcacccgctg ctttacttcg ctacgcgaca
    59521 cgccgagcat ctttaacccg tagccgacgt tttcgcccag cgacatatgc gggaacagag
    59581 cgtatgactg aaacaccatg cagatatcgc gctgctgaat ggaacgatgc gtgacatctt
    59641 cgccatcaat aaagatttgc ccttcactcg gtttttccag cccggcaacc agacgcaaaa
    59701 tggtggtttt gccgcagccg gaagggccaa gcagcgtcac catttgccct tgtgggatgg
    59761 tgagattgat attgtcgata accgtattac tgccgaatcg tttagtgacg ttgcgcagtt
    59821 ccacaaaatt tttctgactc atagtgcgct ccattacgcc tggtttttgg ctttagaacg
    59881 ggagatacgt gcttctccga tcagccagtc aaagaggaaa ataatcgcca gcatcaccac
    59941 gatcagaatc gaaccatagg cgatggctac gccatattcg ccgtcttcaa cgcggttaag
    60001 aatgtaggcg gttgccacgc gggtgtccgg cgtgacgaga aagacgatgg cgctgaccgt
    60061 cgtgatggcc cgcacaaagc tgtagatcag ggccgagaga atggccggac gcagcagcgg
    60121 cagtaaaatg tgggttatgg ttcgcaagga acccgcgcgc aggctgagtg aggcttcatc
    60181 cagcgatttg tcgatctgcc ccaggcctgc aatcccggcg cgaataccca ccggaacgtt
    60241 acgcatcacc atcgagataa tcacaattgc cgccgttccg gtgagataaa ccggagcgct
    60301 gttaaaggca agaatatacg aaacgcccgc caccgtaccc ggtacggcaa agcacagcat
    60361 ggtggtgaac tcgatggtct ttttgccttt gaattgttga cgcaccacca cccaggcgat
    60421 gagcaggcca aagatagcgg taatgggagc ggcaatcccg gcgtagagca gggtatccag
    60481 cagtgaaggc cacgcgccgt cgctcattcc ctgaccaaat agcttgataa agttatccag
    60541 cgtcagggtg tagtccacgc cccagttcac cgtgaagctg ccgtagaaaa tgctgccgta
    60601 gagcagggcg ttaaaggcaa tccatatcgc aagtatggcg accacgctcc acaccagggt
    60661 gaccggaagc ggctggacgt cgccacggta ggatttgccg gaaaccgtga cgtaagagcg
    60721 tttaccaatc cacatatact gtatgcagaa caccagcagc gagaacagca gcaggaaagc
    60781 gccaagggtg ctggcggcct gataatccag ctgcgagccg gtaatataga agtaaatttg
    60841 cgtcgccagt acgtcgaagt taccgccaag taccagcggg ttactgaaat cagccagtga
    60901 ctgtaccacg acgattaaaa aggcattggc gagcgcgggc ttcaacaacg gcacaaacac
    60961 gccgttaaag gtttgccagc ggctggcgcg cagggtgtag gaggcctctt ccagcgaggg
    61021 atgaatggtt ttaatggccc cgtcgaggat cataaatgcc attggcgtga atgccagaac
    61081 ctgtgccagc cagatcccgg taaaaccgta cagccagttg gtgttggtca gcccaaacca
    61141 ctccaccata aattccgtaa catagccgga acgccccatc atgagcgtca cgcctaagcc
    61201 gaccacaaac ggtggcgtaa cgatgggcaa aatggaaaag atgcgaccaa taatggcgct
    61261 gcgtttagcg atgcgcgtgg tataaatcgc cagcaccagg ccgaagaaag cacagcctat
    61321 ccccaccgct atcgacaaga cgatagagtt gacgatcacc tgaacaatat gtgcctgcga
    61381 gagcacggtc ataaacgcca gcggggcgag ctctcccgcg tcgttggtga acatcgggat
    61441 aaaaatggcg atgctcggcc agacgataaa aatgccaatc agcgccacaa tcgcaatcag
    61501 tgagccgatc acgaagcggt cgccgcccag ccattcgagg cgggttaagg ccagcgtcat
    61561 aatcgcgcct agttcgacaa acagcacgat ggcggcgtag cctaaaccgc gtccagccac
    61621 ggtggcgctg atgacgataa acgccataca gagcaatgcc cagccggcat cgaaataatg
    61681 acgactacgc tgttcgcgcg tggcctgacg aaacgggcgg accagcaaca ggctcgggag
    61741 taaataccac agccagctga tattaaaatg cgaccagccg tacgccccga ggatctcatc
    61801 actcgtggat tccagcaggc cataatcgag gctccagctc ggcaacagga caaatgccag
    61861 ccagcccagc aaaacccaaa ggaaaatcgc atcccgcttt ttcacgggat ggagtgcaag
    61921 tgtctgtgac atagcagttc cggtatggtt ggccggggca ggcgtctgcg ctgcacccgg
    61981 caaatgcgcc taatgcgggt ttatttaccc atcttcactt cgctgaccca tttattaatc
    62041 aatgccttac gaacatccgt ggaaccatat ttatccatgt cgtagttgat taatttcagg
    62101 tcgtcgagct tcagcgaatt tggcgacgtt tcggcggtgg tattagtcag gatctgataa
    62161 gacttccctt tcttccacgc cagttcctga gcttcttttg ataataccca gtccacaaac
    62221 agtttggcgt tgtcgaggtt gcgcgcgcct ttcagaatgc tgacgccgcc aatttcgtag
    62281 cccgtacctt cacacgggga aatcaactcc agcggcgcgc cctgttcttt ttccagcgag
    62341 tagtcatgca ggaagccaat accaatcgcc gtttcgccac gggcggcgtt acgtgccggg
    62401 gctatgcctg atttggtgta ctgagagacg ttggcgttca gttgttttag ataatcaaag
    62461 gcctgatctt ccccccacag ctgcgcaaaa gtagccaggg cggtgtaggc agtaccggaa
    62521 ctttgcggat cggcaatttg aatttcgcct ttatattccg gtttggtgag atctttccag
    62581 cacttcggca ccggcaggtt tttctctttc agacgatcgg tgtttacgcc aaaaccgagg
    62641 atccccacat aaacggcaga ggacagattc cctttcactt ttgccggatc gcggaatttc
    62701 tccatgatct gctcaaggtt aggggattta tacgcctgta gcaggcccat ctcgccagcc
    62761 tgcgattgcg ggtctaaagt gccgccgtac cagacgtcag cctgggggtt tttcttttca
    62821 gcatcgactt ttgccagggt gcttccggaa ccgttgcgga taaacgaggt ttttacatca
    62881 tatttctcgc caaacgcttt ggtttcggcc tcacacattt cattggtggc gctgcaatag
    62941 accaccagcc ggccttttgc ctgcgcggca cccgttaaag tcgccagcgc aacggcagaa
    63001 gcaatcagag tagaggtaaa ggtcattttc attgttttat ccttcgagcg tgagtggtgg
    63061 tgatgcccgc catcagtaac ggcatcagca gtaaacccat aagtactgcg gcgaccgaga
    63121 gtaaactgaa cattcccggc cagccgtagc gttcgatgac cagcgacagc ggccagcctg
    63181 ccagcgccgc gccaaggtaa gcgaacaggc cgagaaatcc ggtgattgaa cccgccgcag
    63241 ctttgtgccc acactccacc gccgcaagtc caatcagcat ttgtgggcca aagacgaaaa
    63301 agcccacggt aaaaaagcac actgccagca gagcgtagtg atgaaccggt gccagccaca
    63361 gtgcggcgac tgacaccatc aaccccaacg tgaacagcaa aatcattggc gctcgttgcc
    63421 cgctaaacag caaatccgat ccccatccgg caaataacgc gccgagtaag ccgcccactt
    63481 caaacagcat taccgtggcg ttggcgctga gcaggttgac accgtggctt tccgtcagcc
    63541 agatattgcc ccagtcgtta agcgcaatcc ggatcacata aaccagtaca tatgagacgc
    63601 ccagtagcca gatcagcgga ttttgcagca tggtggtgcg caacatttgc cacagcccca
    63661 tcggcggact ttgttgttcc tgacgtaact ccagcggatc gtgacgccag tgaccaactg
    63721 tcggtaatcc ctcttcctgg ggcgtgcctt tgagttgtag cgttaaccag atccctaatg
    63781 ccatgctgat tatccccggt gtcagcatcg cggcctgcca gccccaccag tgggcggcaa
    63841 aggcgctaat cagcggaata atcgctccgc caatgttgat cgacatattc cagcagcccc
    63901 accagaaacc gcgctcattg cgtgagtacc agtgggtcag caagcgggca cacggtggcc
    63961 atccccagcc ctgaaaaaaa ccgttcagtg tccagaccac aagcagaagc gtaagtgatt
    64021 cgccaaaggc aaacaccacg ttcaataacc cggtggcgaa caagccgacg cccataaaac
    64081 cgcgttgtcc gtggctgtca tgccacagtc cggcggtaaa tttcgacaat ccgtagctga
    64141 gataaaacag cgaacccagc aggccaatgt cgcctttatc cagtccgagg tcggtttgta
    64201 gcgccggaag gacgtaattc acgctttttc gcgtcagata aaatgcggcg tagccaataa
    64261 tcatgcacat cagcaatcgc ggacgtaatg tccggtagca ttgattaatt tctctcgctg
    64321 aacgcgcgtg catggttgtc tccatgttgc tgactatatg aaaatgaaag tgaaagggga
    64381 tgagagaaaa tccgggatac ctaagacttt ttcctggtta tccggcgatt tgttgcaaaa
    64441 atgtgggcaa gttaacaatt acccgcgtgc cgtgctgttt ttcgagcgtt aagtcgccgc
    64501 ccagggcgct gacgcgctca cgcatcccct gaatgccaaa tccagccatt ttcccgctgg
    64561 cgatccccac accgttgtcg ctgacttcca gatgcaatac ctctccctgc tggcgcagga
    64621 taatcgtcac ttcgctggct tcggcgtgtt tacagatgtt attgagcaac tcctgcaata
    64681 accgatacaa ggtaaagcgc accgtttcgt tttctggtgt ggagttcagt tgataggcaa
    64741 actggcaatg aatgccgcgt tcactaaagg caaattcatt aatcagatgc agcaacgctt
    64801 cgcgaaatgt cagttcatca agcgcgggag gccgcaactg gcgcagcaat tggcgggtgg
    64861 agagatgaat gcggcgggcg agctcattga tttggctggc tgcggattga atttgcgccg
    64921 gatcacgcgc ccgcttcacc agttgcgact ggatctgaat ggcggtgatg ttctgaccga
    64981 tttcgtcgtg cagctctcgc gccaggtttt ttcgcgtgtc ctcctcggtg tggatcagct
    65041 tttccgccaa tgcccgccgc gctgccagct cggcctccag ccgttgtcgg taatggtgca
    65101 ggtttagcgc cagatgttgc tggcgactga tggcaatgcc caggccaatt cccagcaagg
    65161 cctgggtcgc gaggaaaatc tccagctcca ccaggttact aaagcccacg ccaatctggc
    65221 gggcgatggt gatcatcatg ctgcccaaaa gcccggagag tacgccacct tgccagccaa
    65281 atttccacgc catcaccaca ttgggcaaaa agaccacgat caacagcaga cgttcaattt
    65341 caggtgacag caccatctga gtgccgatac caataataaa aaacaggcta caccagatga
    65401 ttaaagaggt acgcaagggg ggattatgcg tatcgagtcc caggagctgg tagcgatgct
    65461 gctggcgcag aaactcaaag ataagataga caaacggcgt cagtaatacg ccgccggtaa
    65521 atgaggtcaa acccggtagc attattgggc ctgacataaa aggggagagc agggcggtat
    65581 tcaacaatga agctgccgtg acagtggcta acagcaacgt cagacgctgc cagtaaagtg
    65641 gaaaacggtg ccagaagttt tgcgcaagga gggcaggaaa caggctaata accggcgttg
    65701 ccagcatgag ataaccgttc aatagctgtt cactatggag ccagaacatc atcagcaaag
    65761 gggggagcac caacgcaggc cagtagcggc gagagagcaa aataacaagg gcgagataaa
    65821 ctccctgcgg gagaaacaac accgcctgtt gaccgttatt ggtcagataa aagctgagcg
    65881 tccacagcat cagccagccg gttccccagg ctagcacaat aaacagcaaa atcacccaat
    65941 gacgaagatt gcgcgacatt aatgccccgc cagcagatgg tggtcgagcg caaaatgtac
    66001 cagttcaatg gtgctatggc agttcagttt tcccagcaca ttggcgcgat gcacatgtac
    66061 cgttttatga ctgagttcga gtttaaaggc gatctcttta acgctgtctc ctttgaccag
    66121 taactcaaac acttcacgtt ctcgtggggt gagaatttcc agcgcctggg cgggttgccc
    66181 gccgccgcgt aaagcccgaa tcgcatcggc acagagataa tgcccgccca gcccgaccga
    66241 gcgcaccgcc tgtactaact cttccggccc acagcgtttg gttaaatacc cgctggcacc
    66301 ggcatccagc gcgctttgca caaagattgg cgcgtcataa atgctaagaa taatggcgcg
    66361 aaattgcggt ttttgtgcac gcaggcgctt aagcaaactg aggccgtttt cgtccggcat
    66421 ggcaatatca atgacggcaa cattgacgtc atcacggatt aatgccgacc atgcctgcgc
    66481 ggcactactg tactgaccaa tgacctccag atcgtcttcc agagagagta attgtgcgaa
    66541 gccagagcgc accacaacat ggtcatccac cagcactacc cgaatcatac ttttcttctc
    66601 ttattatcta attctcctat catgcatcac ggcgcggcgc tggcaatcaa ccagcgacag
    66661 atatggaatg gatgtaacaa aatggcaata atttgccgga gcgcgcacat cttatctggc
    66721 ttgtgaaccg gcgggatacg ttcgccaaaa ccgcaaaatg cgcaaactcg ctaaccattg
    66781 ccgatttttt tctaaaaacg ctttgttctt aacaagacct tttaattcct ttatcaaact
    66841 gacccgcaac gaaatactgc tcccataaca agacagggga gcagacaatc atggcaattt
    66901 catcgcgtaa cactcttctt gccgcactgg cattcatcgc ttttcaggcg caggcggtga
    66961 acgtcaccgt ggcgtatcaa acctcagccg aaccggcgaa agtggctcag gccgataaca
    67021 cctttgctaa agaaagtgga gcaactgtgg actggcgcaa gtttgacagc ggagccagca
    67081 tcgtgcgggc gctggcttct ggcgacgtgc aaatcggcaa ccttggttcc agcccgttag
    67141 cggttgcagc cagccaacag gtgccgattg aagtcttctt gctggcgtca aaactgggta
    67201 actccgaagc gctggtggta aagaaaacta tcagcaaacc ggaagatctg attggcaaac
    67261 gcatcgccgt accgtttatc tccaccaccc actacagcct gctggcggcg ctgaaacact
    67321 ggggcattaa acccgggcaa gtggagattg tgaacctgca gccgcccgcg attatcgctg
    67381 cctggcagcg gggagatatt gatggtgctt atgtctgggc accggcggtt aacgccctgg
    67441 aaaaagacgg caaggtgttg accgattctg aacaggtcgg gcagtggggc gcgccaacgc
    67501 tggacgtttg ggtggtgcgc aaagattttg ccgagaaaca tcctgaggtc gtgaaagcgt
    67561 tcgctaaaag cgccatcgat gctcagcaac cgtacattgc taacccagac gtgtggctga
    67621 aacagccgga aaacatcagc aaactggcgc gtttaagcgg cgtgcctgaa ggtgacgttc
    67681 cggggctggt gaaggggaat acctatctga cgccgcagca acaaacggca gaactgaccg
    67741 gaccggtgaa caaagcgatc atcgacaccg cgcagttttt gaaagagcag ggcaaggtcc
    67801 cggctgtagc gaatgattac agccagtacg ttacctcgcg cttcgtgcaa taaaaggagg
    67861 cgcagatgct gcaaatctct catctttacg ccgattatgg cggcaaaccg gtactggaag
    67921 atatcaacct gacgctggaa agcggcgagc tactggtggt gctggggccg tccggctgcg
    67981 gtaaaaccac cctgctgaat ttgattgccg gttttgtgcc ttatcagcat ggcagcattc
    68041 aactggcggg taagcgtatt gaggggccgg gagcagagcg tggcgtagtt tttcagaatg
    68101 aagggctact accgtggcgc aatgtacagg acaacgtggc gttcggcctg caattggcag
    68161 gtatagagaa aatacagcga ctggaaatcg cgcaccagat gctgaaaaaa gtggggctgg
    68221 aaggcgcaga aaaacgctac atctggcagc tttccggtgg tcaacgtcag cgggtgggga
    68281 ttgcccgcgc gctggcggcg aatccccagc tgttattact cgacgaaccg tttggtgcgc
    68341 tggacgcctt cacccgcgac cagatgcaaa ccctgctgct gaaactctgg caggagacgg
    68401 gcaagcaggt gctgttgatt acccacgata tagaagaagc ggtgtttatg gcgactgaac
    68461 tggttctgct ttcacccggc cctggccgcg tgctggagcg gctgccgctc aactttgctc
    68521 gccgctttgt tgcgggagag tcgagccgca gcatcaagtc cgatccacaa ttcatcgcca
    68581 tgcgcgaata tgttttaagc cgcgtatttg agcaacggga ggcgttctca tgagtgtgct
    68641 cattaatgaa aaactgcatt cgcggcggct gaaatggcgc tggccgctct cgcgtcaggt
    68701 gaccttaagc attggcacgt tagcggtttt actcactgta tggtggacgg tggcggcgct
    68761 gcaactgatt agcccgctgt ttttgccgcc gccgcaacaa gtactggcaa aactgctcac
    68821 cattgccgga ccgcagggat ttatggacgc cacgctgtgg cagcatctgg cagccagcct
    68881 gacgcgcatt gtgctggcgc tactcgcggc ggtgttgatc ggcattccgg tcgggatcgc
    68941 catggggcta agcccgacgg tgcgcggcat tctggatccg ataatcgagc tttatcgtcc
    69001 ggtgccgccg ctggcttatt tgccgctgat ggtgatctgg tttggtattg gtgaaacctc
    69061 gaagatctta ctgatctatt tagcgatttt tgcaccggtg gcgatgtcgg cgctggcggg
    69121 ggtgaaaagc gcgcagcagg ttcgcattcg tgctgcccag tcgctgggtg ccagccgtgc
    69181 gcaggtgctg tggtttgtca ttttgcccgg cgcgctgccg gaaatcctca ccggattacg
    69241 tattggtctg ggtgtgggct ggtctacgct ggtggcggcg gagctgattg ccgcgacgcg
    69301 cggtttagga tttatggttc agtcagcggg tgaatttctc gcgactgacg tggtgctggc
    69361 ggggatcgcg gtgattgcga ttatcgcctt tcttttagaa ctgggtctgc gcgcgttaca
    69421 gcgccgcctg acgccctggc atggagaagt acaatgagtg aacgtctgag cattaccccg
    69481 ctggggccgt atatcggagc acaaatttcg ggtgccgacc tgacgcgccc gttaagcgat
    69541 aatcagtttg aacaacttta ccatgcggta ctgcgccatc aggtggtgtt tctgcgcgat
    69601 caagccatta cgccgcagca gcaacgcgcg ctggcccagc gttttggcga actgcatatt
    69661 caccctgttt accctcatgc cgaaggggtt gacgagatca tcgtgctgga tacccataac
    69721 gataatccgc cagataacga caactggcat accgatgtga catttattga aacgccaccc
    69781 gcaggggcga ttctggcagc gaaagagtta ccttcgaccg gcggcgatac gctctggacc
    69841 agcggtattg cggcctatga ggcgctctct gttcccttcc gccagctgct gagcgggctg
    69901 cgtgcggagc atgattttcg taaatcgttc ccggaataca aataccgcaa aaccgaagag
    69961 gaacatcaac gctggcgcga ggcggttgcg aaaaatccgc cgttgctaca tccggtggtg
    70021 cgaacgcatc cggtgagcgg taaacaggcg ctgtttgtga atgaaggctt tactacgcga
    70081 attgttgatg tcagcgagaa agagagcgaa gccttgttag gctttttatt cgcccatatc
    70141 actaaaccgg agtttcaggt gcgctggcgc tggcaaccga atgatattgc gatttgggat
    70201 aaccgtgtga ctcagcacta tgccaatgcc gattacctgc cacagcggcg gattatgcat
    70261 cgggcgacga ttcttgggga taaaccgttt tatcgggcgg ggtaatacga gagtggacgg
    70321 tcctctcgcc cccttgggga gagggttagg gtgagggggc gttcaccgtg ctttcaacag
    70381 gttaactccc cctttctgag aggaaacaaa attaacgcag aatcttcttc tcagccaaat
    70441 ccagcgcaaa gtagctgaaa atcagatccg cacccgcacg cttaatcgaa cctaagcttt
    70501 cgagcacgac tttctcttca tctatagcac ccgccagcgc ggcgaactta atcatcgcat
    70561 actcaccgct cacctgatac gcgccaatcg gcaattcagt acgttcacgc agctcacgca
    70621 cgatgtcgag gtacgctcca gcaggtttaa ccatcaggca gtctgcgccc tgggcttcat
    70681 ccagcagtga ctcacgaatc gcctcacgac ggttcattgg gttcatctga tagcttttgc
    70741 ggtcgccttt taatgcgctt ccggcagctt cacggaacgg accataaaag gaggaggcga
    70801 acttggtcga atacgacata atcgccgtat ctttaaagcc agcagcgtcc agcgcctggc
    70861 gaatcgcctg tacctggcca tccatcgcgg cggaaggggc gatgaagtct gcacctgcag
    70921 cagctgcaac cacggcttgc ttgcctaaat tttccagagt cgcgtcgttg tcgacgccat
    70981 gctcgcacag cacaccgcag tgaccgtgag aagtgtattc acagaagcag gtgtctgaca
    71041 tgacaatcat ttctggcacc gtctgcttgc agatgcgcga catacgcgct accagtccat
    71101 cttcccgcca ggcatcgctg ccggtttcat cggtatggtg agagatgccg aaagtcatca
    71161 cggaacgaat accggcgttg gcgatgcgtt caatttcgcg tgccagatgt ttctctggaa
    71221 tgcgcatcac gccaggcatg gcttcaacgg ctttgtagtc gtcaatttct tcttcaacaa
    71281 agatcggcaa caccaggtcg ttaaggctaa gtgttgtctc ttcaaacata gcgcgcagcg
    71341 caggagattt gcgcaggcga cgagggcgtt ggattaagtc tgtcatggtc tgcctgatgt
    71401 ttgtggaatt aaggtgcaca gtatacctga agcggggctg gagtgtttta cgaaagttgt
    71461 cacgatgttg acaagaagag aatgaaagag agaccagggc atctttgtaa tagaaatagc
    71521 aataacttca tattcactga aaatattttt taatttttat ttataggtgc tggttattat
    71581 ttttgtcaat ttggttgaac tgtcaaaaat tgaattactt aaagttcatt gcttattgat
    71641 aaattatttg tctgatttgt tgttttggta tttttaggat ctataattca aaattaatga
    71701 tttcattaac gtgttgaaat gcagtgtttt tgtcaactta ttctttgtgc gtatagatat
    71761 ttttatatat gtccgcaatc ggacttaggg gaaagaataa ttgaaccatt cctctataac
    71821 gccgcataat cgtcaacgcg aaacataaga tgttttctat gcattgataa ttgatggatc
    71881 aacttattac gtccctgagg agggatgaca aatgcactcc tggaaaaaga aacttatagt
    71941 atcacaatta gcattggctt gcactctggc tatcacctct caggctaatg cagcgaccaa
    72001 cgatatttct ggtcaaactt acaatacttt ccatcactac aacgacgcca cctatgctga
    72061 tgacgtttac tatgatggtt atgtaggctg gaacaactat gccgctgata gctattacaa
    72121 cggcgatatc tacccggtca ttaataacgc taccgttaac ggcgtgattt ctacctacta
    72181 tctggacgac ggtatttcta ccaataccaa cgccaatagt ctgacaatca aaaacagcac
    72241 tattcacggt atgattacct ctgagtgcat gactactgat tgtgctgatg accgtgctac
    72301 tggttatgtt tatgatcgtc tgacactgag cgttgataat tcaacgatcg atgacaacta
    72361 cgagcattat acttacaacg gtacctataa taatgccgct gacactcatg ttgtagatgt
    72421 ttacgatatg ggtactgcta ttacactgga tcaggaagtt gatctgtcca tcactaataa
    72481 ctctcatgta gcaggtatta cgctgactca gggttatgag tgggaagata ttgacgacaa
    72541 cacagtcagc actggcgtaa acagcagcga agtgtttaat aacactatta ctgttaaaga
    72601 ttctactgtg acctctggtt catggactga tgaaggtact actggttggt ttggccatac
    72661 tggtaatgcc agcaactata gcaacacgct gactgcagac gatgttgcaa ttgccgcaat
    72721 cgcaaatccg tatgctgata atgcgatgca gactacagta actttagaca actcaacact
    72781 gatgggtgat gttgttttct ccagtaattt cgatgaaaac ttcttcccgc aaggtgctaa
    72841 cagctatcgc gatgctgatg gtgatgtaga taccaacggt tgggatggca cagaccgtat
    72901 ggatgtgact ctgaacaacg gcagcaagtg ggttggcgct gcaatgtctg ttcatatggt
    72961 tgatgaagat ggtgatggtt cttacgacgg atatgctgtt ggtactgaag caactgcaac
    73021 tctgctcgat attgcagcta acagcctgtg gccttcatca actgtcggtg ttgataacat
    73081 caatactcaa tatgacgaaa atggccatat cgtaggaaac gaagtttacc agagcggttt
    73141 gtttaatgtg actttgaacg gtggttcaga gtgggataca acaaaatctt ctctgattga
    73201 tactttaagt attaacagcg gttcccaagt taatgttgca gactctcgtc tgatctctga
    73261 ctggcggttc taacctgaac atcggtgaag acggtcatgt agcgactaat accctgacca
    73321 tcgacaatag taccgttaaa atgtctgatg atgtttctgc gggctggggt ttagaagatg
    73381 ctgcactgta cgcaaatacc atcaccgtaa ctaacgacgg tctgttggat attaacgttg
    73441 atcagttcga tgctaacccg ttccaggccg ataccctgaa tctgaccagt accactgata
    73501 ctaacggcaa cattcacgct ggtgtattcg atatccatag cagtgattac gtaatggata
    73561 ccgatctggt caacgatcgt accaacgata ctaccaagtc aaactacggt tatggcttaa
    73621 tcgcaatgaa ctctgatggt cacctgacta ttaacggtaa cggcgataac gacaacactg
    73681 cttctatcga agctggtcag aacgaagttg ataacaacgg tgaccatgtt gcagccgcga
    73741 ccggtaacta caaagttcgt atcgacaacg ctactggtgc tggttctatc gctgactaca
    73801 acggcaacga gctgatctac gtcaacgaca aaaacagcaa cgcgaccttc tctgctgcta
    73861 acaaagctga cctgggtgca tacacctatc aggctgaaca gcgcggtaac accgttgttc
    73921 tgcaacagat ggagttgacc gactacgcta acatggcgct gagcatccca tctgcgaaca
    73981 ccaatatctg gaacctggaa caagacaccg ttggtactcg tttgaccaac tctcgtcatg
    74041 gcctggctga taacggcggc gcatgggtaa gctacttcgg tggtaacttc aacggcgaca
    74101 acggcaccat caactatgat caggatgtta acggcatcat ggtcggtgtt gataccaaaa
    74161 ttgacggtaa caacgctaag tggatcgtcg gtgcggctgc aggcttcgct aaaggtgaca
    74221 tgaatgaccg ttctggtcag gtggatcaag acagccagac tgcctacatc tactcttctg
    74281 ctcacttcgc gaacaacgtc tttgttgatg gtagcttgag ctactctcac ttcaacaacg
    74341 acctgtctgc aaccatgagc aacggtactt acgttgacgg tagcaccaac tccgacgctt
    74401 ggggcttcgg tttgaaagcc ggttacgact tcaaactggg tgatgctggt tacgtgactc
    74461 cttacggcag catttctggt ctgttccagt ctggtgatga ctaccagctg agcaacgaca
    74521 tgaaagttga cggtcagtct tacgacagca tgcgttatga actgggtgta gatgcaggtt
    74581 ataccttcac ctacagcgaa gatcaggctc tgactccgta cttcaaactg gcttacgtct
    74641 acgacgactc taacaacgat aacgatgtga acggtgattc catcgataac ggtactgaag
    74701 ggtctgcggt acgtgttggt ctgggtactc agttcagctt caccaagaac ttcagcgcct
    74761 ataccgatgc taactacctc ggtggtggtg acgtagatca agactggtcc gcgaacgtgg
    74821 gtgttaaata tacctggtaa tattcttcac tccgaagaaa tactggtaat ttaatctaaa
    74881 taatgcccgt caaggatttg acgggcatta ctgcaaagga cgcgcaaatg ttatctgtag
    74941 ttaaacctct tcaggaattt ggtaagctcg ataaatgttt gtccagatac ggtacgcgct
    75001 tcgagtttaa taatgaaaag caagttatat tttccagtga tgtcaataac gaagatactt
    75061 tcgttatttt agagggagtt atctctctgc gtagagaaga aaacgtactt atcggtatta
    75121 cccaggctcc ttatattatg gggctggctg atggtttaat gaaaaacgat ataccataca
    75181 aattaatatc agaaggaaat tgtacgggat atcatctacc agccaaacaa accattacgc
    75241 ttattgaaca aaatcaactc tggcgagacg ctttttactg gttagcctgg caaaatagaa
    75301 ttctggaatt acgcgacgtg cagctcattg ggcataattc ctacgaacaa atccgcgcaa
    75361 cattattatc aatgattgac tggaatgaag aattgcgatc acgtattggt gtgatgaatt
    75421 atatccatca acgtacacgc atatcgcgtt ctgtcgtcgc agaagttctc gctgctttgc
    75481 gtaaaggcgg ctatatcgaa atgaataaag gcaaactggt cgctatcaac cgtttgcctt
    75541 cagagtatta atcaggatgc ggggataacc aacggtttat tcccgcttag ctcggtcacc
    75601 agatcattga tgccatcgct catattttta aagcgcgtca acggcgaacg agtgaccacc
    75661 acaaacgcgc caatattctt ctgcgggatc atcgccatat aggtaatgaa accgccacca
    75721 ccacctgtct tctgaataat ccccggacga ccctctttcg gggccatata tacccagcct
    75781 aaaccaagcg catccgcttt gccggggaca tccatgccaa tcactttcgt aaactgcgcg
    75841 cgctgataaa tcaacgtctg catgcgatct gcctggttac tgcgctgata aaaatcagat
    75901 gacaaatact gctgcatcca gcgcatcata tcgccaggcg tggaatagac gccgccgctg
    75961 ccaattgccg ccagcgtgtt attgcacgga ctggcaccgc gctcagcaac cattaaacgg
    76021 cggcactgat ccggtgaggg agtgtaggtg gtgtctttca tccccaatgg acgggtaatc
    76081 tgctcttcaa acaactgggt ataaggcttg ccggacgcat tcgccagtgc atctgccagc
    76141 aggtcgaacg caaggttaga gtacgccgcc tgactgcctg gcgcggcttt cagcttcgcc
    76201 gtagaaaggt atttccagcg ttgctcgcgc gttggccaga caaacaccgg acgatgtgcc
    76261 gcgccaccgg gctgttcacg gggcagggcg ctggtatggg ttgccagatt gaccagcgta
    76321 atgggcgtcc cattgtaggt tggcacgcgt gcgcctggcg gagcatattt acttagcgga
    76381 tcgtttaact tcacggtccc ctggtcgagc aatttcacca gcatctcact ggtcattagc
    76441 ttggtgaggg aagcaatacg cacgaccgaa tccagctgcg ggcgaacgtt attaccaggt
    76501 cgcgtttcgc cataactgcg aaagacgcgc tggttaccgt cgataacaac cagcgccatc
    76561 cccgtggcac cgctgccgta aaaaatatga tcggcataac gatcgacaat atcagaggca
    76621 aactccggtt cagtgatcgg ctgcgccgca tggacggagg tcaatgacgc cgcacacagc
    76681 acggcagaaa aaagcagact acgtttcaac ggtggtgtcc ataaattgaa tgaaggtaac
    76741 tgagcgtatt tatactactc attgatggat tgcaaagggc gaattagtgc aataatagtg
    76801 ttaaaaacgt aacggcgcgt aaacacgatc tttttcctgc tcaccaatgc ctgcatttgt
    76861 gatcctgcgc gtgttaatcc gcttatgtaa acaatctttg gtttgtgaaa tccgtcgaaa
    76921 caattcttat ggccaggctg gcgaactaag cgccttgcta tgagtcacaa tgggcggttt
    76981 catttggctg acgctttgta gcggtcatgc ggttaataca cagcagttgt taaaacgata
    77041 agaagttagc aggagtgcat atgtttaagt cttttttccc aaagccggga acgttttttc
    77101 tctcggcctt tgtttgggca ttgattgccg ttatcttctg gcaagccggt gggggggact
    77161 gggtggcgcg tatcaccggc gcttccgggc agatcccgat tagcgccgcg cgtttctggt
    77221 cgttggattt cctgattttt tacgcttact acattgtttg cgtaggactt tttgcattgt
    77281 tctggtttat ctacagcccg caccgttggc aatactggtc aatactcggt actgcactga
    77341 tcatcttcgt cacctggttt ttggtggaag ttggggttgc cgtcaacgcc tggtatgcac
    77401 cgttctatga tctgattcaa accgcgctaa gttcgccgca taaagtcact atcgaacaat
    77461 tttaccgcga agtgggcgtc tttctgggga ttgcgctgat cgcggtagtg atcagtgtgc
    77521 tgaataactt ctttgtcagt cactacgtgt tccgctggcg tacggcgatg aacgaatatt
    77581 acatggcgaa ctggcaacaa ctgcgtcata tcgaaggcgc cgcacagcgt gtgcaggaag
    77641 acaccatgcg ttttgcttca acgctggaga atatgggtgt cagttttatc aacgccatca
    77701 tgacgttgat cgccttcctg ccggtgttgg taacgctctc cgcgcatgtg ccggagctgc
    77761 cgattatcgg gcacattccg tatggtctgg tgattgccgc tatcgtctgg tcgctgatgg
    77821 ggaccggatt gctggcagtg gtagggatca aactgccggg gctggagttt aaaaaccagc
    77881 gtgtagaggc tgcctaccgt aaagagctgg tttatggtga agacgatgcc acgcgcgcga
    77941 cgccgccaac ggtacgcgag ctgtttagcg ccgtacggaa aaactatttc cgcctctatt
    78001 ttcactatat gtatttcaac atcgctcgca ttctctattt gcaggtcgat aacgttttcg
    78061 gtttgttctt gctgtttccg tcaattgttg ccggtacgat tacgctcggc ctgatgacgc
    78121 agattaccaa cgtttttggt caggttcgcg gtgctttcca gtacctgatt aactcatgga
    78181 ccacactggt tgagttgatg tctatctaca aacgtctgcg cagctttgaa catgagctgg
    78241 atggtgacaa aattcaggaa gtaacccata ccttgagcta aaaaggagaa gtaatgtcgc
    78301 gagtcaatca tctgtcttcg ctttcatttt tggcggttct ggttttagcg gggtgtagca
    78361 gtcaggcgcc acagccgttg aaaaagggcg aaaaagcgat agatgtggct agcgtggtgc
    78421 ggcagaagat gcctgcgagc gtgaaagacc gcgatgcctg ggcgaaagat ctcgccacca
    78481 cctttgaaag tcaggggctg gcaccgacgc tggaaaacgt ctgctcggtg ctggcggtgg
    78541 cgcagcagga gtcaaattat caggcagatc cggccgttcc gggcttaagc aagatcgcct
    78601 ggcaagagat tgaccgtcgt gctgaacgga tgcacattcc tgcttttctg gttcatactg
    78661 cgctaaaaat caaatcgcca aacggcaaaa gttatagcga acggttagat tcggtgcgta
    78721 cagaaaagca attgagcggg atttttgacg atctgatcag catggtgcca atggggcaga
    78781 cgttgtttgg ttcgcttaat ccggtgcgca ccggtgggcc gatgcaggtc agcattgctt
    78841 ttgccgaaca gcataccaaa gggtatccgt ggaaaatgga cggtacagtc cgtcaggaag
    78901 tctttagtcg ccgcggcggg ttgtggtttg gtacttacca tttactgaat tatcccgcca
    78961 gttatagcgc accgatatac cgttttgctg attttaacgc tggctggtac gccagccgta
    79021 atgccgcgtt tcagaacgcg gtcagtaagg ccagcggcgt gaagctggca ctggacggtg
    79081 atttaattcg ttacgacagt aaagaacccg ggaaaaccga actggcaacg cgcaaactgg
    79141 cggcaaaact ggggatgagc gacagcgaaa ttcgtcgtca gctagagaaa ggtgacagct
    79201 tctcttttga ggagacggcg ctgtacaaga aagtttatca acttgccgaa gcgaaaacgg
    79261 gtaaatcact cccccgcgaa atgttgcctg gcattcaact ggaaagcccg aaaatcaccc
    79321 gcaacctgac tacggcctgg tttgcgaagc gcgtagacga acggcgggcg cgttgtatga
    79381 aacagtgaac aaacaggaat gtcatgccag ataaggcgtt ttaggccgta tctggcaacg
    79441 taaatcttac tggcggcgat gtcgccagtg cagccatagc gccaccactg caaaaatgag
    79501 gcagcctact aaaaacggga tcagcccgaa aatggtgccg acacctaaac caatttccac
    79561 ccgtgggcga cctgtttcct ggacctgcat taaacgctca tagacgccgg gcgcatgcac
    79621 taacaggttc agcaattgcg ccccagccca aaagcaaaac agcacaaaca acgcataggc
    79681 aatgttgcca ggcgttgaag aggcattgcg atattttccg ctaaacagcg attttgacag
    79741 ggtgaaatca gccataaaaa cctccggaag tcatctgctt ctcttctggt gtgataccag
    79801 cggcttatgg caagtctgac aggtcattta gattgtcaat atcagaatca tggtaaattg
    79861 atgttgggaa tattcccgaa gcgtgaatct tcgttgcgag tcacaaaata gcaactttaa
    79921 gttaaattta ttaacaattg cgaaaatgtt gtctacacca cagacggttg ttgcagaata
    79981 tgcaaggatg ttgtttttcg ttaacggagc agccatgaat ctgcctgtaa aaatccgccg
    80041 tgactggcac tactatgcgt tcgccattgg ccttatattc attcttaatg gcgtggtggg
    80101 gttactggga tttgaagcaa aaggttggca gacctatgcc gtcggtctgg tgacgtgggt
    80161 gattagtttc tggctggcgg ggttgattat tcgtcgtcgc gatgaagaaa ctgaaaacgc
    80221 ccaataagaa tattatcggg cgttaaaata ttacattgtg gttttcaatg cgttatccgc
    80281 agcgtgacgc tccagcgcca gctcaatcaa acgtgtgatc agatcggtgt aacccagacc
    80341 gctggcttgc cacagcttcg gatacatact gatgttggtg aagccaggca gtgtgttgat
    80401 ctcgttgatc accacttcgt tctctggggt taaaaacacg tctacacgcg ccatgcctgc
    80461 gcatcccaac gtctgataag cctgaacggc aatcgcccgg atcttatcgt tgatttctgg
    80521 cgcaatggct gccggaacta ccactttcgc gccatcttcg tcaatgtact tggtgtcgta
    80581 ggcatagaaa tcgctggtga gtacgatctc gccacaggtg ctggcttgcg gattgtcgtt
    80641 gcccagaact gcgcattcga tctcacgacc tttgatccct tgctcaacga tcactttatg
    80701 atcgaactcg aacgccagat cgacggcaat tgcgtattgt tcttcactgg ttactttgct
    80761 gacaccaaca gaagagccct gattagccgg ttttacaaac agtggtaacc ccagtttaga
    80821 ctccacttcg gcaaaactga tgttgtgacg attagcgcgc gtcagggtaa taaatggcgc
    80881 aatgttcagc ccggcatcgc gtagcagacg tttggtgaca tctttatcca tacaggctgc
    80941 tgaagccaga acatcagaac ctacaaacgg taaattggcg acccgcagca ttccctgcaa
    81001 ggaaccatct tcgcccagcg taccgtggac aatcgggaaa atgacatcca ccgtcggcaa
    81061 cggctgaccg ttttgcgcgt cgataagctg atgctcatgt ttacctggca cctgggcgag
    81121 gctggtcgcc gaagggcgca acgcaatatg ggcaggatcg tctgcattta gcagataatt
    81181 gctggcatcg ctgacgtgcc attgcccttg tttatcaatg cccagcagca caacgtcgaa
    81241 gcgactttta tcaatggcat cgacaatgtt ttttgccgat tgcagagaca cttcatgttc
    81301 cgctgattta ccaccaaaaa cgattcctac ccgcagtttt tccatcttaa aaacctatcc
    81361 cgtctaacac aaagtgcata cattaccacg acaaaacggg ggattcgcgg ccttctgaaa
    81421 gattgttgca atcttctgct gacaaagcgt gcaacgtact ggtgaagaaa gtgcgttatc
    81481 tcaaagatgt gcgcaagatc acaaaaatga tgaacgggaa gctaatttat tcctggctta
    81541 aatggccatg cggtgagttt ttttctctta attataagtt aacgaagaga atatatttca
    81601 taacttttat ttataataaa ggttgataat taaaagccta tgttttgtgt gggtaattat
    81661 ttaaataaga gaaacgtttc gctggtaatc aaacaaaaaa tatttgcgca aagtatttcc
    81721 tttgccataa aaataatacc tccagacact atgaagttgt gaaacataat gttaacttct
    81781 ccatactttg gataaggaaa tacagacatg aaaaatctca ttgctgagtt gttatttaag
    81841 cttgcccaaa aagaagaaga gtcgaaagaa ctgtgtgcgc aggtagaagc tttggagatt
    81901 atcgtcactg caatgcttcg caatatggcg caaaatgacc aacagcggtt gattgatcag
    81961 gtagaggggg cgctgtacga ggtaaagccc gatgccagca ttcctgacga cgatacggag
    82021 ctgctgcgcg attacgtaaa gaagttattg aagcatcctc gtcagtaaaa agttaatctt
    82081 ttcaacagct gtcataaagt tgtcacggcc gagacttata gtcgctttgt ttttattttt
    82141 taatgtattt gtacatggag aaaataaagt gaaacaaagc actattgcac tggcactctt
    82201 accgttactg tttacccctg tgacaaaagc ccggacacca gaaatgcctg ttctggaaaa
    82261 ccgggctgct cagggcgata ttactgcacc cggcggtgct cgccgcttaa cggctgatca
    82321 gaccgccgct ctgcgtgatt ctcttagcga taaacctgca aaaaatatta ttttgctgat
    82381 tggcgatggg atgggggatt cggaaattac tgccgcacgt aattatgccg aaggtgcggg
    82441 cggctttttt aaaggtatcg atgccttacc gcttaccggg caatacactc actatgcgct
    82501 gaataaaaaa accggaaaac cggactacgt caccgactcg gctgcatcag caaccgcctg
    82561 gtcaaccggt gtcaaaacct ataacggcgc gctgggcgtc gatattcacg aaaaagatca
    82621 cccaacgatt ctggaaatgg caaaagccgc aggtctggcg accggtaacg tttctaccgc
    82681 agagttgcag gatgccacgc ccgccgcgct ggtggcgcat gtgacctcgc gcaaatgcta
    82741 cggtccgagc gcgaccagtg aaaaatgtcc gggtaacgct ctggaaaaag gcggaaaagg
    82801 atcgattacc gaacagctgc ttaacgctcg tgccgacgtt acgcttggcg gcggcgcaaa
    82861 aacctttgct gaaacggcaa ccgctggtga atggcaggga aaaacgctgc gtgaacaggc
    82921 acaggcgcgt ggttatcagt tggtgagcga tgctgcctca ctgaattcgg tgacggaagc
    82981 gaatcagcaa aaacccctgc ttggcctgtt tgctgacggc aatatgccag tgcgctggct
    83041 aggaccgaaa gcaacgtacc acggtaatat cgataagccc gcagtcacct gtacgcctaa
    83101 tccgcaacgt aatgacagtg taccgaccct ggcacagatg accgacaaag ccattgaatt
    83161 gttgagtaaa aatgagaaag gctttttcct gcaagttgaa ggtgcgtcaa tcgataaaca
    83221 ggatcatgct gcgaatcctt gtgggcaaat tggcgagacg gtcgatctcg atgaagccgt
    83281 acaacgggcg ctggaattcg ccaaaaagga tggtaacacg ctggtcatag tcaccgctga
    83341 tcacgcccac gccagccaga ttgttgcgcc ggataccaaa gctccgggcc tcactcaggc
    83401 gctaaatacc aaagatggcg cagtgatggt gatgagttac gggaactccg aagaggattc
    83461 acaagaacat accggcagtc agttgcgtat tgcggcgtat ggcccgcatg ccgccaatgt
    83521 cgttggactg accgaccaga ccgatctctt ctacaccatg aaagccgctc tggggctgaa
    83581 ataaaaccgc tcccggcagt gaattttcgc tgccgggtgg tttttttgct gttagcaacc
    83641 agacttaatg tcagatcacg ggcgcatacg ctcatggtta aaacatgaag agggatggtg
    83701 ctatgaaaat tacattactg gttaccttgc ttttcggtct ggttttttta accaccgtcg
    83761 gcgctgccga gagaacctta accccacaac aacagcgtat gacctcctgt aatcagcagg
    83821 cgacggcaca ggcgttgaaa ggggatgctc gtaagaccta catgagtgat tgcctgaaga
    83881 acagcaagtc tgcgcctggt gaaaaaagtt tgacgccaca gcagcaaaag atgcgcgaat
    83941 gtaataatca agcaacgcaa caatcactga aaggtgatga tcgtaataag tttatgagcg
    84001 cctgcctcaa gaaagccgcc tgatacctga tagtgctaac g
//
LOCUS       DABSYT020000011        82671 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.011, whole genome shotgun sequence.
ACCESSION   DABSYT020000011 DABSYT020000000
VERSION     DABSYT020000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 82671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.011"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            180..347
                     /gene="yhfL"
                     /locus_tag="ID725_RS05155"
                     /old_locus_tag="pgaptmp_001031"
     CDS             180..347
                     /gene="yhfL"
                     /locus_tag="ID725_RS05155"
                     /old_locus_tag="pgaptmp_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGV7849682.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            643..1980
                     /gene="frlA"
                     /locus_tag="ID725_RS05160"
                     /old_locus_tag="pgaptmp_001032"
     CDS             643..1980
                     /gene="frlA"
                     /locus_tag="ID725_RS05160"
                     /old_locus_tag="pgaptmp_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGV7849683.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            2001..3023
                     /gene="frlB"
                     /locus_tag="ID725_RS05165"
                     /old_locus_tag="pgaptmp_001033"
     CDS             2001..3023
                     /gene="frlB"
                     /locus_tag="ID725_RS05165"
                     /old_locus_tag="pgaptmp_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGV7849684.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            3074..3901
                     /gene="frlC"
                     /locus_tag="ID725_RS05170"
                     /old_locus_tag="pgaptmp_001034"
     CDS             3074..3901
                     /gene="frlC"
                     /locus_tag="ID725_RS05170"
                     /old_locus_tag="pgaptmp_001034"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGV7849685.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            3901..4686
                     /gene="frlD"
                     /locus_tag="ID725_RS05175"
                     /old_locus_tag="pgaptmp_001035"
                     /pseudo
     CDS             3901..4686
                     /gene="frlD"
                     /locus_tag="ID725_RS05175"
                     /old_locus_tag="pgaptmp_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            4786..5517
                     /gene="frlR"
                     /locus_tag="ID725_RS05180"
                     /old_locus_tag="pgaptmp_001036"
                     /gene_synonym="yhfR"
     CDS             4786..5517
                     /gene="frlR"
                     /locus_tag="ID725_RS05180"
                     /old_locus_tag="pgaptmp_001036"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGV7849686.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5648..6652)
                     /gene="trpS"
                     /locus_tag="ID725_RS05185"
                     /old_locus_tag="pgaptmp_001037"
     CDS             complement(5648..6652)
                     /gene="trpS"
                     /locus_tag="ID725_RS05185"
                     /old_locus_tag="pgaptmp_001037"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGV7849687.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(6645..7403)
                     /gene="gph"
                     /locus_tag="ID725_RS05190"
                     /old_locus_tag="pgaptmp_001038"
     CDS             complement(6645..7403)
                     /gene="gph"
                     /locus_tag="ID725_RS05190"
                     /old_locus_tag="pgaptmp_001038"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGV7849688.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(7396..8073)
                     /gene="rpe"
                     /locus_tag="ID725_RS05195"
                     /old_locus_tag="pgaptmp_001039"
     CDS             complement(7396..8073)
                     /gene="rpe"
                     /locus_tag="ID725_RS05195"
                     /old_locus_tag="pgaptmp_001039"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGV7849689.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(8091..8927)
                     /gene="dam"
                     /locus_tag="ID725_RS05200"
                     /old_locus_tag="pgaptmp_001040"
     CDS             complement(8091..8927)
                     /gene="dam"
                     /locus_tag="ID725_RS05200"
                     /old_locus_tag="pgaptmp_001040"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGV7849690.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(9034..10320)
                     /gene="damX"
                     /locus_tag="ID725_RS05205"
                     /old_locus_tag="pgaptmp_001041"
     CDS             complement(9034..10320)
                     /gene="damX"
                     /locus_tag="ID725_RS05205"
                     /old_locus_tag="pgaptmp_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGV7849691.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(10412..11500)
                     /gene="aroB"
                     /locus_tag="ID725_RS05210"
                     /old_locus_tag="pgaptmp_001042"
     CDS             complement(10412..11500)
                     /gene="aroB"
                     /locus_tag="ID725_RS05210"
                     /old_locus_tag="pgaptmp_001042"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGV7849692.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(11557..12078)
                     /gene="aroK"
                     /locus_tag="ID725_RS05215"
                     /old_locus_tag="pgaptmp_001043"
     CDS             complement(11557..12078)
                     /gene="aroK"
                     /locus_tag="ID725_RS05215"
                     /old_locus_tag="pgaptmp_001043"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGV7849693.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(12479..13717)
                     /gene="hofQ"
                     /locus_tag="ID725_RS05220"
                     /old_locus_tag="pgaptmp_001044"
     CDS             complement(12479..13717)
                     /gene="hofQ"
                     /locus_tag="ID725_RS05220"
                     /old_locus_tag="pgaptmp_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGV7849694.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(13629..14033)
                     /gene="hofP"
                     /locus_tag="ID725_RS05225"
                     /old_locus_tag="pgaptmp_001045"
     CDS             complement(13629..14033)
                     /gene="hofP"
                     /locus_tag="ID725_RS05225"
                     /old_locus_tag="pgaptmp_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGV7849695.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTLDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(14023..14463)
                     /gene="hofO"
                     /locus_tag="ID725_RS05230"
                     /old_locus_tag="pgaptmp_001046"
     CDS             complement(14023..14463)
                     /gene="hofO"
                     /locus_tag="ID725_RS05230"
                     /old_locus_tag="pgaptmp_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGV7849696.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(14447..14986)
                     /gene="hofN"
                     /locus_tag="ID725_RS05235"
                     /old_locus_tag="pgaptmp_001047"
     CDS             complement(14447..14986)
                     /gene="hofN"
                     /locus_tag="ID725_RS05235"
                     /old_locus_tag="pgaptmp_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGV7849697.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(14970..15764)
                     /gene="hofM"
                     /locus_tag="ID725_RS05240"
                     /old_locus_tag="pgaptmp_001048"
     CDS             complement(14970..15764)
                     /gene="hofM"
                     /locus_tag="ID725_RS05240"
                     /old_locus_tag="pgaptmp_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGV7849698.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            15884..18436
                     /gene="mrcA"
                     /locus_tag="ID725_RS05245"
                     /old_locus_tag="pgaptmp_001049"
     CDS             15884..18436
                     /gene="mrcA"
                     /locus_tag="ID725_RS05245"
                     /old_locus_tag="pgaptmp_001049"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGV7849699.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(18601..19161)
                     /gene="nudE"
                     /locus_tag="ID725_RS05250"
                     /old_locus_tag="pgaptmp_001050"
     CDS             complement(18601..19161)
                     /gene="nudE"
                     /locus_tag="ID725_RS05250"
                     /old_locus_tag="pgaptmp_001050"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGV7849700.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            19481..21616
                     /gene="igaA"
                     /locus_tag="ID725_RS05255"
                     /old_locus_tag="pgaptmp_001051"
     CDS             19481..21616
                     /gene="igaA"
                     /locus_tag="ID725_RS05255"
                     /old_locus_tag="pgaptmp_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGV7849701.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            21681..22349
                     /gene="yrfG"
                     /locus_tag="ID725_RS05260"
                     /old_locus_tag="pgaptmp_001052"
     CDS             21681..22349
                     /gene="yrfG"
                     /locus_tag="ID725_RS05260"
                     /old_locus_tag="pgaptmp_001052"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGV7849702.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            22360..22761
                     /gene="hslR"
                     /locus_tag="ID725_RS05265"
                     /old_locus_tag="pgaptmp_001053"
     CDS             22360..22761
                     /gene="hslR"
                     /locus_tag="ID725_RS05265"
                     /old_locus_tag="pgaptmp_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGV7849703.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            22786..23664
                     /gene="hslO"
                     /locus_tag="ID725_RS05270"
                     /old_locus_tag="pgaptmp_001054"
     CDS             22786..23664
                     /gene="hslO"
                     /locus_tag="ID725_RS05270"
                     /old_locus_tag="pgaptmp_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGV7849704.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23727..25451)
                     /gene="yhgE"
                     /locus_tag="ID725_RS05275"
                     /old_locus_tag="pgaptmp_001055"
     CDS             complement(23727..25451)
                     /gene="yhgE"
                     /locus_tag="ID725_RS05275"
                     /old_locus_tag="pgaptmp_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGV7849705.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            25830..27452
                     /gene="pckA"
                     /locus_tag="ID725_RS05280"
                     /old_locus_tag="pgaptmp_001056"
     CDS             25830..27452
                     /gene="pckA"
                     /locus_tag="ID725_RS05280"
                     /old_locus_tag="pgaptmp_001056"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGV7849706.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(27528..28880)
                     /gene="envZ"
                     /locus_tag="ID725_RS05285"
                     /old_locus_tag="pgaptmp_001057"
     CDS             complement(27528..28880)
                     /gene="envZ"
                     /locus_tag="ID725_RS05285"
                     /old_locus_tag="pgaptmp_001057"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGV7849707.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(28877..29596)
                     /gene="ompR"
                     /locus_tag="ID725_RS05290"
                     /old_locus_tag="pgaptmp_001058"
     CDS             complement(28877..29596)
                     /gene="ompR"
                     /locus_tag="ID725_RS05290"
                     /old_locus_tag="pgaptmp_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGV7849708.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            29824..30300
                     /gene="greB"
                     /locus_tag="ID725_RS05295"
                     /old_locus_tag="pgaptmp_001059"
     CDS             29824..30300
                     /gene="greB"
                     /locus_tag="ID725_RS05295"
                     /old_locus_tag="pgaptmp_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGV7849709.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            30397..32718
                     /gene="yhgF"
                     /locus_tag="ID725_RS05300"
                     /old_locus_tag="pgaptmp_001060"
     CDS             30397..32718
                     /gene="yhgF"
                     /locus_tag="ID725_RS05300"
                     /old_locus_tag="pgaptmp_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGV7849710.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIITDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            33156..33383
                     /gene="feoA"
                     /locus_tag="ID725_RS05305"
                     /old_locus_tag="pgaptmp_001061"
     CDS             33156..33383
                     /gene="feoA"
                     /locus_tag="ID725_RS05305"
                     /old_locus_tag="pgaptmp_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGV7849711.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            33400..35721
                     /gene="feoB"
                     /locus_tag="ID725_RS05310"
                     /old_locus_tag="pgaptmp_001062"
     CDS             33400..35721
                     /gene="feoB"
                     /locus_tag="ID725_RS05310"
                     /old_locus_tag="pgaptmp_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGV7849712.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            35721..35957
                     /gene="feoC"
                     /locus_tag="ID725_RS05315"
                     /old_locus_tag="pgaptmp_001063"
     CDS             35721..35957
                     /gene="feoC"
                     /locus_tag="ID725_RS05315"
                     /old_locus_tag="pgaptmp_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGV7849713.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            36160..37038
                     /gene="rpnA"
                     /locus_tag="ID725_RS05320"
                     /old_locus_tag="pgaptmp_001064"
     CDS             36160..37038
                     /gene="rpnA"
                     /locus_tag="ID725_RS05320"
                     /old_locus_tag="pgaptmp_001064"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGV7849714.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYPDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(37065..37835)
                     /gene="bioH"
                     /locus_tag="ID725_RS05325"
                     /old_locus_tag="pgaptmp_001065"
     CDS             complement(37065..37835)
                     /gene="bioH"
                     /locus_tag="ID725_RS05325"
                     /old_locus_tag="pgaptmp_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGV7849715.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            37873..38556
                     /gene="gntX"
                     /locus_tag="ID725_RS05330"
                     /old_locus_tag="pgaptmp_001066"
     CDS             37873..38556
                     /gene="gntX"
                     /locus_tag="ID725_RS05330"
                     /old_locus_tag="pgaptmp_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGV7849716.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            38615..39190
                     /gene="nfuA"
                     /locus_tag="ID725_RS05335"
                     /old_locus_tag="pgaptmp_001067"
     CDS             38615..39190
                     /gene="nfuA"
                     /locus_tag="ID725_RS05335"
                     /old_locus_tag="pgaptmp_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGV7849717.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            39551..40867
                     /gene="gntT"
                     /locus_tag="ID725_RS05340"
                     /old_locus_tag="pgaptmp_001068"
     CDS             39551..40867
                     /gene="gntT"
                     /locus_tag="ID725_RS05340"
                     /old_locus_tag="pgaptmp_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGV7849718.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(40912..42996)
                     /gene="malQ"
                     /locus_tag="ID725_RS05345"
                     /old_locus_tag="pgaptmp_001069"
     CDS             complement(40912..42996)
                     /gene="malQ"
                     /locus_tag="ID725_RS05345"
                     /old_locus_tag="pgaptmp_001069"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGV7849719.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRGHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(43006..45399)
                     /gene="malP"
                     /locus_tag="ID725_RS05350"
                     /old_locus_tag="pgaptmp_001070"
     CDS             complement(43006..45399)
                     /gene="malP"
                     /locus_tag="ID725_RS05350"
                     /old_locus_tag="pgaptmp_001070"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGV7849720.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            46011..48716
                     /gene="malT"
                     /locus_tag="ID725_RS05355"
                     /old_locus_tag="pgaptmp_001071"
     CDS             46011..48716
                     /gene="malT"
                     /locus_tag="ID725_RS05355"
                     /old_locus_tag="pgaptmp_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGV7849721.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            48783..49061
                     /locus_tag="ID725_RS05360"
                     /old_locus_tag="pgaptmp_001072"
     CDS             48783..49061
                     /locus_tag="ID725_RS05360"
                     /old_locus_tag="pgaptmp_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGV7849722.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            49052..49537
                     /locus_tag="ID725_RS05365"
                     /old_locus_tag="pgaptmp_001073"
     CDS             49052..49537
                     /locus_tag="ID725_RS05365"
                     /old_locus_tag="pgaptmp_001073"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGV7849723.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(49587..50615)
                     /gene="rtcA"
                     /locus_tag="ID725_RS05370"
                     /old_locus_tag="pgaptmp_001074"
     CDS             complement(49587..50615)
                     /gene="rtcA"
                     /locus_tag="ID725_RS05370"
                     /old_locus_tag="pgaptmp_001074"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGV7849724.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(50619..51845)
                     /gene="rtcB"
                     /locus_tag="ID725_RS05375"
                     /old_locus_tag="pgaptmp_001075"
     CDS             complement(50619..51845)
                     /gene="rtcB"
                     /locus_tag="ID725_RS05375"
                     /old_locus_tag="pgaptmp_001075"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGV7849725.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            52033..53631
                     /gene="rtcR"
                     /locus_tag="ID725_RS05380"
                     /old_locus_tag="pgaptmp_001076"
     CDS             52033..53631
                     /gene="rtcR"
                     /locus_tag="ID725_RS05380"
                     /old_locus_tag="pgaptmp_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGV7849726.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(53613..54371)
                     /locus_tag="ID725_RS05385"
                     /old_locus_tag="pgaptmp_001077"
     CDS             complement(53613..54371)
                     /locus_tag="ID725_RS05385"
                     /old_locus_tag="pgaptmp_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGV7849727.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(54388..55218)
                     /gene="glpG"
                     /locus_tag="ID725_RS05390"
                     /old_locus_tag="pgaptmp_001078"
     CDS             complement(54388..55218)
                     /gene="glpG"
                     /locus_tag="ID725_RS05390"
                     /old_locus_tag="pgaptmp_001078"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGV7849728.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(55263..55589)
                     /gene="glpE"
                     /locus_tag="ID725_RS05395"
                     /old_locus_tag="pgaptmp_001079"
     CDS             complement(55263..55589)
                     /gene="glpE"
                     /locus_tag="ID725_RS05395"
                     /old_locus_tag="pgaptmp_001079"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGV7849729.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            55779..57284
                     /gene="glpD"
                     /locus_tag="ID725_RS05400"
                     /old_locus_tag="pgaptmp_001080"
     CDS             55779..57284
                     /gene="glpD"
                     /locus_tag="ID725_RS05400"
                     /old_locus_tag="pgaptmp_001080"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGV7849730.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(57499..58101)
                     /locus_tag="ID725_RS05405"
                     /old_locus_tag="pgaptmp_001081"
     CDS             complement(57499..58101)
                     /locus_tag="ID725_RS05405"
                     /old_locus_tag="pgaptmp_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849731.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(58101..59093)
                     /locus_tag="ID725_RS05410"
                     /old_locus_tag="pgaptmp_001082"
     CDS             complement(58101..59093)
                     /locus_tag="ID725_RS05410"
                     /old_locus_tag="pgaptmp_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGV7849732.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(59119..60627)
                     /locus_tag="ID725_RS05415"
                     /old_locus_tag="pgaptmp_001083"
     CDS             complement(59119..60627)
                     /locus_tag="ID725_RS05415"
                     /old_locus_tag="pgaptmp_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGV7849733.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(60752..63199)
                     /gene="glgP"
                     /locus_tag="ID725_RS05420"
                     /old_locus_tag="pgaptmp_001084"
     CDS             complement(60752..63199)
                     /gene="glgP"
                     /locus_tag="ID725_RS05420"
                     /old_locus_tag="pgaptmp_001084"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGV7849734.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(63218..64651)
                     /gene="glgA"
                     /locus_tag="ID725_RS05425"
                     /old_locus_tag="pgaptmp_001085"
     CDS             complement(63218..64651)
                     /gene="glgA"
                     /locus_tag="ID725_RS05425"
                     /old_locus_tag="pgaptmp_001085"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGV7849735.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(64651..65946)
                     /gene="glgC"
                     /locus_tag="ID725_RS05430"
                     /old_locus_tag="pgaptmp_001086"
     CDS             complement(64651..65946)
                     /gene="glgC"
                     /locus_tag="ID725_RS05430"
                     /old_locus_tag="pgaptmp_001086"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGV7849736.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(65964..67937)
                     /gene="glgX"
                     /locus_tag="ID725_RS05435"
                     /old_locus_tag="pgaptmp_001087"
     CDS             complement(65964..67937)
                     /gene="glgX"
                     /locus_tag="ID725_RS05435"
                     /old_locus_tag="pgaptmp_001087"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGV7849737.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(67934..70120)
                     /gene="glgB"
                     /locus_tag="ID725_RS05440"
                     /old_locus_tag="pgaptmp_001088"
     CDS             complement(67934..70120)
                     /gene="glgB"
                     /locus_tag="ID725_RS05440"
                     /old_locus_tag="pgaptmp_001088"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGV7849738.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(70393..71496)
                     /gene="asd"
                     /locus_tag="ID725_RS05445"
                     /old_locus_tag="pgaptmp_001089"
     CDS             complement(70393..71496)
                     /gene="asd"
                     /locus_tag="ID725_RS05445"
                     /old_locus_tag="pgaptmp_001089"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGV7849739.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            71688..72281
                     /gene="yhgN"
                     /locus_tag="ID725_RS05450"
                     /old_locus_tag="pgaptmp_001090"
     CDS             71688..72281
                     /gene="yhgN"
                     /locus_tag="ID725_RS05450"
                     /old_locus_tag="pgaptmp_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGV7849740.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(72327..73622)
                     /locus_tag="ID725_RS05455"
                     /old_locus_tag="pgaptmp_001091"
     CDS             complement(72327..73622)
                     /locus_tag="ID725_RS05455"
                     /old_locus_tag="pgaptmp_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGV7849741.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(73529..75653)
                     /locus_tag="ID725_RS05460"
                     /old_locus_tag="pgaptmp_001092"
                     /pseudo
     CDS             complement(73529..75653)
                     /locus_tag="ID725_RS05460"
                     /old_locus_tag="pgaptmp_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(75793..77133)
                     /gene="gntU"
                     /locus_tag="ID725_RS05465"
                     /old_locus_tag="pgaptmp_001093"
     CDS             complement(75793..77133)
                     /gene="gntU"
                     /locus_tag="ID725_RS05465"
                     /old_locus_tag="pgaptmp_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGV7849742.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(77137..77664)
                     /gene="gntK"
                     /locus_tag="ID725_RS05470"
                     /old_locus_tag="pgaptmp_001094"
     CDS             complement(77137..77664)
                     /gene="gntK"
                     /locus_tag="ID725_RS05470"
                     /old_locus_tag="pgaptmp_001094"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGV7849743.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(77803..78798)
                     /gene="gntR"
                     /locus_tag="ID725_RS05475"
                     /old_locus_tag="pgaptmp_001095"
     CDS             complement(77803..78798)
                     /gene="gntR"
                     /locus_tag="ID725_RS05475"
                     /old_locus_tag="pgaptmp_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGV7849744.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(79022..79717)
                     /gene="yhhW"
                     /locus_tag="ID725_RS05480"
                     /old_locus_tag="pgaptmp_001096"
     CDS             complement(79022..79717)
                     /gene="yhhW"
                     /locus_tag="ID725_RS05480"
                     /old_locus_tag="pgaptmp_001096"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGV7849745.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(79840..80877)
                     /gene="yhhX"
                     /locus_tag="ID725_RS05485"
                     /old_locus_tag="pgaptmp_001097"
     CDS             complement(79840..80877)
                     /gene="yhhX"
                     /locus_tag="ID725_RS05485"
                     /old_locus_tag="pgaptmp_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGV7849746.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            81211..81699
                     /gene="yhhY"
                     /locus_tag="ID725_RS05490"
                     /old_locus_tag="pgaptmp_001098"
     CDS             81211..81699
                     /gene="yhhY"
                     /locus_tag="ID725_RS05490"
                     /old_locus_tag="pgaptmp_001098"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGV7849747.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
     gene            81910..>82671
                     /locus_tag="ID725_RS05495"
                     /old_locus_tag="pgaptmp_001099"
     CDS             81910..>82671
                     /locus_tag="ID725_RS05495"
                     /old_locus_tag="pgaptmp_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HGV7849748.1"
                     /translation="MGTAAISHLRYDLNKYALSLRKTATLASTFFIESPLVRFEYLQE
                     IENTINDITHRFNSSYDINEKARLINELKMESETARKEYQLFRQGNYDKYITTDIFEE
                     HGLIKYVNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAHGANNFYESLS
                     PLFFNEYDSGPIRELYRVIAKKMGGDINSADYAYSIVDFSITAYGGYSGMKIVPKYNR
                     LIRPSLGNRPGTGRLFHYTSVDFKNKFSLKPTPLKI"
BASE COUNT        20061 a        22344 c        20636 g        19630 t
ORIGIN      
        1 tcattaacaa aaatcatttg tttttgcccc gttatattca ccgttacagc gacttacaaa
       61 attaagtcat tcactcactg acgccgatta aatattcctg tatcgttctt aatgagcagt
      121 ctacttatat tcatataatc aatgaatatt aatcaatata taatacatag ggaatgtaaa
      181 tgaacaaatt tattaaagtt gcactggtag gtgcagtact ggctacgtta actgcatgta
      241 ctggtcatat tgaaaaccgt gataagaact gctcttacga ctacctgctg cacccagcaa
      301 tttctatttc taaaatcatt ggcggttgcg gtcctactgc acagtaagtc tctgacagaa
      361 atcagctaac gctgatttaa ttgcctgacg ccctcgggat tacccgaggg cgtatttgtt
      421 tctatatcct tcttgctccc cacccgtaaa aatattttca ctttaatttc aattggataa
      481 ccacattcat cagccacagc gcgatcctgt gcgaaattat gtgatcttcc tccacattac
      541 ataacatcat atgttgttat attcatcatg cattgtcatg tcacctttta aatgactgca
      601 aactctcccc tacaacgtgt tggacaagaa aaaggtatcg ctatgggaag ccaggaactc
      661 caacgcaagc tcggattttg ggccgttctt gcaatcgccg tcgggacaac cgtcggctcc
      721 ggtatttttg tatctgtggg tgaagtggca aaagcggcgg gcacgccgtg gctgacggtg
      781 ctcgcatttg tcatcggcgg gttaattgtg atcccgcaaa tgtgcgtata tgcggaacta
      841 tccaccgctt atccggaaaa tggcgcagat tatgtttatc tgaaaaatgc cggaagccga
      901 ccgctggctt tcctctccgg ctgggccagc ttctgggcca acgatgcgcc gtcattgtcg
      961 attatggcgc tggcgattgt cagcaatctt ggctttttaa cgcctatcga tccgttgctc
     1021 ggtaaattta tcgccgccgg attaattatc gcctttatgc tgctgcacct gcgctccgtt
     1081 gaaggcggcg cagcgtttca gacgctaatt accatcgcca aaattatccc gttcactatc
     1141 gtcattggtc ttgggatctt ctggtttaaa gcggagaatt ttgccgcccc tgctaccact
     1201 gcgattggcg caacgggcag ctttatggcg ctgctggcgg ggatctctgc caccagttgg
     1261 tcgtataccg gcatggcctc tatctgttat atgaccggcg aaattaaaaa ccccggcaaa
     1321 accatgccac gagcgctgat tggttcctgt ctgctggttc tggtgctcta caccctgctg
     1381 gcgctggtga tttccggcct gatgcccttc gacaaactcg ccaattctga aacgccgatt
     1441 tccgacgccc tgacctggat ccccgcactc ggcagcaccg ctgggatctt tgttgccatc
     1501 acggcgatga tcgtcattct tggttcgctt tccagctgcg tgatgtacca gccgcggctg
     1561 gaatacgcga tggcgaaaga caacctgttc ttcaaatgct tcggccatgt gcatccgaaa
     1621 tacaacacgc cggatgtctc catcattttg caaggggcgc tggggatctt cttcatcttc
     1681 gtttccgatc tcaccagcct gctgggctat ttcaccctgg taatgtgttt caaaaatacc
     1741 ctcaccttcg gctccatcat ctggtgtcgt aaacgcgacg attacaaacc gctgtggcgt
     1801 actccggctt tcgggctgat gacccccctc gccattgcgt caagcctcat tctggtcgcc
     1861 tcaacctttg tctgggcacc gattcccggc cttatctgcg ccgtcatcgt tattgccact
     1921 ggtctgcctg cttacgcctt ctgggcgaag cgtagccgcc agctcaacgc tttgtcgtaa
     1981 tgttatctgg agaaaataaa atgttggata ttgataaaag cactgtggac tttctggtca
     2041 ccgaaaatat ggttcaggaa gtggaaaaag ttctcagcca tgacgttccg ctggtgcaca
     2101 ccatcgtgga agagatggtg aagcgcgaca tcgatcgtat ttatttcgtt gcctgcggat
     2161 cgccactcaa cgcggcgcaa acggcgaaac atctggcaga tcgcttttcc gatcttcagg
     2221 tttacgccat ttccggctgg gagttctgcg ataacacccc atatcgcctc gacgctcgtt
     2281 gcgcggtgat tggcgtttct gactacggta aaaccgaaga ggtaatcaaa gcgctggagc
     2341 tgggccgggc ctgcggcgca ctcactgcgg cgttcaccaa acgcgcggat agcccgatta
     2401 cctcggcggc ggaatttagc attgattatc aggccgactg tatctgggaa attcatctgc
     2461 tgctctgcta cagcgtggtg ctggagatga tcacccgcct cgcgccgcac gcggaaatcg
     2521 gcaagatcaa aaacgatctc aaacagctgc cgaatgcgct cggttatctg gtacgcactt
     2581 gggaagaaaa aggccgccag cttggtgaac tggccagcca gtggccgatg atttacacgg
     2641 ttgctgcagg cccgctgcgc ccgctgggtt acaaagaagg catcgtgacg ctgatggaat
     2701 ttacctggac gcacggctgt gtgattgaga gcggtgagtt ccgtcacggc ccgctggaga
     2761 ttgtcgaacc gggcgttccg ttcctgttcc tgctcggcaa tgatgaaagt cgccacacca
     2821 ccgaacgcgc cattaacttt gttaaacagc gtactgacaa cgtgatcgtc atcgattacg
     2881 ccgcaatttc gcaaggactg cacccgtggc tggcaccgtt cctgatgttc gtgccaatgg
     2941 agtggctctg ctactacctg tctatttaca aagatcacaa cccggatgaa cgccgctatt
     3001 acggtggtct ggtggaatat taatcctctc tcccggcccg gcaacgggcc ggatttacgc
     3061 aaggagttac ccgatgaaaa caggtatgtt tacctgcggc caccagcggc tgcctattga
     3121 acatgcattc cgtgatgcaa gcgagctggg ttacgatggc atcgaaattt ggggcggccg
     3181 cccgcacgcg ttcgcgccgg acttaaaagc gggcggcatc aaacaaatca aggcgctggc
     3241 gcagacgtat cagatgccga ttatcggtta cacgccagaa accaacggct atccgtataa
     3301 catgatgctg ggcgatgaac atatgcgtcg tgaaagcctg gacatgatca agctggcgat
     3361 ggatatggca aaagagatga acgcgggtta tacgctgatt tctgcggccc acgcgggcta
     3421 tctcacgccg cctaacgtta tctgggggcg gctggcagag aacttaagcg aactgtgtga
     3481 gtacgcggaa aacatcggca tggatctgat cctcgaaccg ttaacgccgt atgaatcgaa
     3541 cgtcgtgtgt aatgctaatg atgtgcttca tgcgctggcg ctggtgcctt cgccgcgcct
     3601 gttcagcatg gtcgacattt gcgcgccgta tgtccaggcg gaaccggtga tgagttattt
     3661 cgacaaactg ggcgataaat tacgtcatct gcatattgtc gacagcgacg gggccagcga
     3721 cacgcattac attcccggcg aaggcaaaat gccgctgcgg gaactgatgc gcgatattat
     3781 tgatcggggc tatgagggat actgcacagt ggaactggta accatgtata tgaacgagcc
     3841 cagactttac gcccgccagg cgctggaacg ctttcgcgcg ctgctgccgg aggatgagtg
     3901 atgaaaaccc tggcgacaat cggcgataac tgcgtcgata tctacccgca actgaataaa
     3961 gcgttttctg gcggtaatgc ggtgaacgtg gcggtgtact gcactcgcta cggtataaag
     4021 ccgggatgca ttacctgggt tggtgacgat gactacggca caaagcttaa gcaggatctc
     4081 gccagcatgg gcgtcgatat cagccatgtc catacgaaac acggcgttac cgcacaaact
     4141 caggtggaac tgcacgacaa tgatcgcgtt tttggcgact acaccgaagg cgtgatggcc
     4201 gactttgccc tgagtgaaga ggattacgcc tagctggcgc agtatgacat tgtgcacgcg
     4261 gcaatctggg gacatgcgga agacgcattc ccacagctgc acgctgcggg caaacttacc
     4321 gctttcgacg tctccgacaa gtgggacagc ccgctctggc agacactggt gccgcatctc
     4381 gattttgcct ttgcctccgc accgcaagaa gacgaagcgc tgcgtctgaa gatgaaagcg
     4441 attgttgccc gtggcgcagg cgtggtgatt gtcacgttgg gtgaaaacgg cagtattgcc
     4501 tgggatggcg tgcagttctg gcgtcaggct cctgaacagg tgacggttat cgacaccatg
     4561 ggtgccggag attcgttcat tgccggattc ctttgcggct ggtctgcggg gatgacatta
     4621 ccgcaggcga tggcgcaggg aacggcgtgc gcggcacaaa ctattcagta ccacggtgcc
     4681 tggtaggcat aacgttggcg tgagcatctt cacgccaacg tgctgttact tgccggaaaa
     4741 cgaccctata atccgtgtaa ttcattcttt atttcagggt cgattatgtc agctacggac
     4801 cgctactctc atcaactcct ctacgctacc gtccgccagc ggctgctgga tgatatcgcg
     4861 cagggggttt accaggccgg gcaacagatc cctaccgaaa atgagctttg tacacaatat
     4921 aacgtcagcc gcattaccat tcgcaaagcc atcagcgact tagtggcaga cggcgtactg
     4981 atccgctggc agggcaaagg cacctttgta caaagccaga aggtcgaaaa cgccctgctg
     5041 accgtcagtg gttttaccga ttttggcgtc tcacaaggca aggcgacgaa agagaaagtg
     5101 atcgagcaag aacggatcag cgccgcgccg ttttgcgaaa agctcaatat ccccggtaac
     5161 agcgaagtgt tccatctttg ccgggtgatg tatctcgata aagagccgct atttattgat
     5221 agttcgtgga tcccgctgtc gcgttatcct gactttgatg agatttacgt cgaaggaagc
     5281 tccacctatc agttatttca ggagcgtttt gacacgcgag tggtcagcga caaaaagacc
     5341 atcgatatct ttgccgccac ccgcccgcag gcaaaatggc tgaaatgcga actgggcgaa
     5401 ccgttgtttc gcatcagcaa aatcgccttt gatcagaatg acaaaccggt gcacgtctcc
     5461 gaactcttct gccgcgccaa ccgcatcacc ttaacaattg ataataaaag acattaaccg
     5521 taggccggat aagatgcacc agcatcgcat ccggcgatgc cggcgcactg aattttacat
     5581 cccgcacgtt cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccgagcgt
     5641 tttgatatta cggcttcgcc acaaaaccaa tcgcttcgta caccgctttt agcgtacggg
     5701 aagcgtgcgc gctggctttt tctgcgccgt ctttcatcac ctgttgcagg aaggcttcat
     5761 cgttgcggaa acggtggtaa cgttcctgca attcagtcag cataccggaa acggcatcag
     5821 ccacttcgcc tttcagatga ccatacatct tgccttcgaa ctgtttttcc agttccggga
     5881 tgctctggcc cgttaccgca gaaaggatat ccagcaggtt ggaaacgccc gctttgttct
     5941 gcacatcgta gcgaactacc ggcggctcgt cggagtcggt gaccgcgcgt ttgattttct
     6001 tcactaccga tttcggatct tccagcaggc caataacgtt attgcggtta tcgtcagact
     6061 tggacatctt cttggtcggc tccagcagcg acattacgcg cgcgccggat ttcggaataa
     6121 acggctccgg caccttaaag atatcgccgt acagcgcgtt gaaacgctgg gcgatatcac
     6181 ggctcagttc caggtgctgt ttctggtctt cacccaccgg taccagatta gtttgataca
     6241 gcaggatgtc cgctgccatc agcaccggat agtcaaacag accagcgttg atgttctcgg
     6301 cataacgcgc agatttatct ttaaactgcg tcatgcggct cagttcgccg aagtaggtat
     6361 agcagttcag tgcccagcct aactgcgcat gttccggtac gtgggactga acaaaaatgg
     6421 tgcttttctc aggatcgata ccacaagcca gatacaaggc cagcgtatcc agcgtcgctt
     6481 tacgcagctt ctgtgcatcc tggcgcacgg tgatcgcgtg ttggtcaacg atacagtaaa
     6541 tgcaatggta gtcatcctgc atgtttaccc actgacgcag cgcacccatg tagttaccaa
     6601 tagtcaattc acctgagggc tgtgcgccac taaaaacgat gggcttagtc atttttcgat
     6661 tcctgatttt cgctatgcgg aagccctaat gcgggcagaa ggtcatttat agactgataa
     6721 attacatcag gctggctgag atcgatagcc tcgccgtagt tatatccgta ggttaagcca
     6781 actgatgggc aacctgccgc ttttgctgcc tgaatatcat tgcgtgagtc gccaacaaac
     6841 agcatctgtt gtggagcaat tcccatccgc tcagccacca gtaacagcgg gtccggatgc
     6901 ggttttttgt tttgcacatc atcaccacca atcaccacgc tgaagtattt ggcgatatct
     6961 aaggcttcga gcagcggcgc gacgaacggc gtcggtttgt tggtgaccag gcctagcggc
     7021 aggcctttag cctgcaacgc gcccaacgta tcggcaacgt gcgggaacaa aaacgtcccc
     7081 tcttcggcaa cctcgccata gtaacgatcg aacagtttac gcagaatacg tacctgttct
     7141 tctgccggaa tgtcgtcatc aacgggcggt ttacccattg ttttacgcag agtcgcacgt
     7201 tcctgacgcg cccaggtcaa tgcgcgctcc atcagaacat ctgcgccgtt accaatccag
     7261 gtaataacgc gttcttcacc tgcgacgggc aactccagcg catacagcgc catatctacc
     7321 gcagcagcaa gaccgggagc actgtccacc agcgtaccgt cgagatcaaa agcgacgccg
     7381 cgaatatctt caaacttact catgacttac ctttgccagt tcactgcgca tttcatcaat
     7441 gacttttttg tagtctggct ggtcgaagat tgccgaaccg gcaacgaaca tatccgcgcc
     7501 cgccgcggcg atttcgccaa tgttgttcac cttcacgcca ccgtccactt ccagacgaat
     7561 gtcaaagcca gactcgtcga tacgacgacg tacttcgcgc agtttatcca gtgtttgagg
     7621 aatgaaagat tgaccgccga aaccagggtt gacggacatc agcaggatca catccagctt
     7681 atccatcacg taatccagat agctcagagg tgtcgccggg ttaaatacca gacccgcttt
     7741 acagccattt tctttaatca gttgcagcgt gcggtcaaca tgctcggagg cttctggatg
     7801 aaaggtaatg atgctggcac cagcggcagc gaaatcaggc acaatgcgat cgaccggttt
     7861 caccatcagg tgtacgtcga taggggcggt aatgccgtag ttacgcaagg atttcagcac
     7921 cattggccca atcgtcagat tgggaacgta gtggttatcc atgacgtcaa aatgcacgac
     7981 atcagcgcca gctgccaggg cttttgcggt atcttcaccc aggcgggcaa aatcagccga
     8041 cagaattgag ggggcaatca aatactgttt catccgcttc tccttgagaa ttattttttc
     8101 gcgggtgaaa cgactcctgg tttgtacaaa gccagcagtt cgtccacctt tttacgtgtg
     8161 ccgccgttgc tgcttatact gcgtcgaact ttgacgacat gcaattttgc gcgctgatac
     8221 cactcacgcg ttaacatcgt atcgtgattg gagatcagca caggaatatg gcgctcaacc
     8281 agaccttcgg cgatctccgc cagatgcgct tgttgttcaa gcgtaaaact gtttgtgtga
     8341 tacgccgtaa agttggcggt cgcagacagc ggtgcataag gtggatcgca atagacgaca
     8401 gatgcatcat ctgcgcgcgc catgctatcg gcgtaagact cacaatagaa aaaggcattc
     8461 tgcgcttttt cagcgaagtg atacaactct gcttccggga aatagggttt tttgtagcgg
     8521 ccaaacggca cattaaactc accgcgcaga ttgtaacgac acaggccgtt gtaaccgtag
     8581 cggttcaaat ataaaaacag taccgcccga cggaacggat cctggctttt gttgaactct
     8641 tcgcggaact gatagtaaac ctcggcgcaa tttgtttcgg gaacaaacag ctcgcgtgcg
     8701 gcctgtacgt actcatcagt acgcatcttc acaatgttat agagactgat caggtcgcta
     8761 ttgatatcgg caaggatata acgagaaaag tcggtgttga gaaacaccga cccggcacct
     8821 acaaaaggct caaccagaca ttcgcccttg ggcaaatgcc gtttaatatc atcaagcagg
     8881 ggatacttgc cccctgccca cttcaaaaaa gcgcgatttt tcttcatgct gactaactaa
     8941 ttacaccttc tccggctgtg gagaaagctc cgacagcatc cagcgcttca ggcaactccc
     9001 gcaacgataa accattgcgg gagataacct tgattacttc agatcggcct gtacctgacg
     9061 cagcggtttc gcccacgggt ttttggcctg aacatctgct ggcaatgtag ataccgcttt
     9121 tttcgcctct tctttcgaag cgtacacgcc agaaaccagg acataccacg gctgaccatt
     9181 acgcgtcgtt tcatagacaa cgtagttttt cagattctct ttcttcgccc aaccgttcag
     9241 gttgtcgtag ttagaggaac tgctcagctg cagagtgtaa tggctggacg gtgccgattt
     9301 caacgaaccc acattacctg cggtcttccc tccagcggct ggtgttgccg tggtttgcgc
     9361 cggggatgcc gtctgtactg gagccgtagt cgccgtctct tttggtgctg gcgtagaagt
     9421 cgcagccggt gccttcgtgc tcgccacagg agcagctggt tcagtacgct tcggcgtctg
     9481 tgctaccggc ttcggttccg ttttcacggt tgcttgcggt tttttcggtt caatcaccgc
     9541 ctgctgacga gctggacgcg tagtggccgg acgttcagca gtttgcgttt tcgccgtgtc
     9601 acgcgatgca ttgccattgc gtactggcgc gacagttgct ggttcagtcg gcaatgtaga
     9661 attgaccgcc acattgttca actgttgctg attttgtggc tgggtcagcg cattgttcag
     9721 gtcaccctgc acttcaacac gttgttgacc atccgttgcc gccggggttt gcccttgagt
     9781 cggcgtagaa gagatcggtg gcagagaaac atcctgctga gtattttccg cagacgtggt
     9841 tcccggcgct ggctgcacgc cattcgcctg atcggtcgca ttgccagcaa gatcaatact
     9901 cttctcgcca gacgcggttt gatcgctgga agtggtcgag ggggctttta gcgcagaacc
     9961 gataccgatg atcaacagca gtagaaccag aatgccgacg cccatcatca tatactgacg
    10021 agaagcgggt ttactggctg cttttttgcg cttacgcgga cgacgctcta cgcgctcttc
    10081 atccacggtt tcatcttcgg attcgtcaat ttcttcttcg atttccggtt cctcattgcg
    10141 ctctttttgc gcacgagtcg gacggcgatc gtcagtgtca tcaagttcaa tatcatcaaa
    10201 attgatctgc ggttcgccac gttcagtacg ctcagaacgt tcagaagatt gacgagaacg
    10261 accagtacga cgatcgctgg gatcgggttt cagctcgtct tctggtttga attcatccat
    10321 ttaacacccc actaaaaggt taatgcttac cacgttgcaa ttaacctgaa gctaatagac
    10381 cgcttgataa gcggcctgac ctttcttgtt gttacgctga ttgacaatcg gcaatggcgt
    10441 taagaacaag ctcgtgcgaa acgccgctgc gaacttcact cttaccaatt gccaacggaa
    10501 gaattaagcg catctctccc gcaaggactt tcttgtcacg cagcatatgc ggtaaatacg
    10561 cctgcgcgga catttcgcgc ggcccattga ccggtaaccc agcccgcgtg agcagagtta
    10621 taatacgctg cgtttcggca gaactaaact gcccgagacg ttccgacgtc cgcgccgcca
    10681 tcaccatacc cgcagcgacc gcttcaccat gtaaccaatt gccatacccc atttcagctt
    10741 caatggcatg accaaaggtg tgtcccagat tcagtaaagc acgtaacccg gtttcgcgct
    10801 cgtcggcggc gacaacttct gccttcagtt cacaacaacg gcgaatacag tacgccattg
    10861 ccggaccgtc cagacgcaac aacgcatcca gattctcttc cagccagtta aaaaacgtac
    10921 cgtcaagaat aatgccgtat ttgatgactt ctgccagccc cgacgctaac tcacgcgggg
    10981 gaagcgtttt cagacagtcg agatccacca ccactgaagc aggctggtag aacgcgccaa
    11041 tcatgttttt accgagggga tggttgaccg cagttttgcc gccaacggag gaatcgacct
    11101 gcgacagtaa cgtcgtcggg acttgaatga aacgaacacc gcgctgataa ctcgccgccg
    11161 cgaagccggt cagatcgccc actacgccgc cgccaagcgc caccagcgta gtatcgcgac
    11221 catgcggctt ttgtaacaac gccgtaaaga cggtatcgag tacagccagg cttttatact
    11281 gctcgccgtc aggaaggata acgctatcga cgttaacacc cgcctgttca agtacgccgc
    11341 ggaccttatc gagatacaga ggagccaggg tttcgttggt gaccaacatg acctgctcgc
    11401 ccgatttcag cggtaagaat gaagctggtt cattaaacaa accagatgcg atggtaattg
    11461 ggtaactacg ttccccgaga gtaacgacaa tcctctccat aacgcgacat ccaccttaat
    11521 tactgtaccc gcagacgagt gtatataaag ccagaattag ttgctttcca gcatgtgaat
    11581 aatctggttt gcaaccactt tagcgctttg atcatcagta cgaatggtca cgtcggcaat
    11641 ctcttcatac agcggattgc gttcattggc caacgcttcc agaacttcac gcggcggtgt
    11701 ttcaacgtgc agcaacgggc gttttttatc acgctgcgtg cgtgcaagtt gcttttcgat
    11761 cgtcgtttca agataaacga caacgccacg agcggaaaga cggttacgcg tttcacggga
    11821 tttcacagag ccgccgccag tagccagcac aataccctgt ttctcggtca actcattgat
    11881 gaccttttct tcgcgatcgc ggaagccttc ttcgccttct aaatcgaaaa cccagcccac
    11941 atcagctccg gttcgtttct caatctcttg atcggaatcg taaaattcca tattgagttg
    12001 ttgagctaac tggcgcccaa tagtgctttt tccggcaccc ataggcccaa ccagaaagat
    12061 attgcgtttc tctgccattt tttcggtact actaagacta ttcgttaatg ataaacccgc
    12121 ttcgctcaga gagctccgca ggacatgaac tgaaacctca taagatattg cgagagtcag
    12181 actgaaaatt atctcaatac tccagcgggt ttggcaactg aataaatcac ccatccctct
    12241 gcatatctgg tcgctgcaag cgcgctgcct tgctaccacc gctctggcga taaatcaccg
    12301 ggtaagatta gcgtaaaaaa gacagcaaaa tgccgcctga atgataaatc atcatcatgg
    12361 aaggcaggca gcgaagagca ccaactctca aatcggtact ccttgtatgc taaatacctg
    12421 cgcgcgtcaa atagatgaaa cacgttcagc gtaaaaacat taccgctttt acggctgttt
    12481 actcactgga aaccagtcgt ggcgtgataa acaccactaa ctcgcgtcgt tcatcttctt
    12541 ttccgtcatg acgaaataat tgcccgaacc agggaatgtc gccaagcaac ggcacgctat
    12601 cctgacccga tttattttta cgggtaaaaa tgccgcccag cgccaacgtt tctccgcttt
    12661 tgacctcgac ctgcgtttcg atctcctgct tatcaatcgc cagcacttcg ccatcggcct
    12721 gctgtagcac ctgccccgga acgttctggc tgatgtgtaa tttcagccgg atgcgacctt
    12781 tttgtaacac cgtgggcgtg acctccatcc ccaggacggc ctctttaaat tccaccgacg
    12841 tcgcgccact ttccccgctg gaaacctgat atggaatttc gctcccctgt ttaatgctgg
    12901 caggctgaag atgtgaggcc agcagacgcg gactggcgat aatatccagc tgctgttttt
    12961 gttcgagcgc ggaaagctca agatccagca aacgtccgtt gatgcgccca atgttaaaac
    13021 cgacatgcgt tgtcgccgtc gctacggaga ggtcgctacc aagcgtggtg acttgcccaa
    13081 cgccaccagc gtgttgcgca tcggccagcg tccatttcac gcctaactca cgcaaacttt
    13141 tttcattaat ggtgacaata tgcgccgaca gctcaacctg cccgaccggc agatccattt
    13201 gcgctaccca ctgttcaagg gcgcttaacg ccgttttgtt atctcgtagc aaaaggcgat
    13261 tggtgcgttt atcgacggtc atactccctt tggcactcag tagcttctcc ccagctttcg
    13321 ccagctctcc ggcgtcggcg tattgcagag taatattgcg attttccagc ggcagatttg
    13381 cctgcgcccg cgcctgctcc gcttcctgac gggcgatatt gtcattctgc caggcaatgg
    13441 aatgcactga gagaatgttg ccctcctggc gcgttatcag tccggcgctt ttcactacag
    13501 tttgtagtgc ctgcttccag ggaacatctg ttagatgtaa cgacaccgta ccgctgacgt
    13561 ctggcgacac caccaggttc aacttctcct gttcagccag cgcctgcaac acctgagcta
    13621 ccggaacgtc atccaccatc agcgtcactt tttgcggctt tgccgcctgt acgccgggta
    13681 tcagcatcaa cagtagtgcg gctatccatt gcttcatttg tatctccttg ccgttgccac
    13741 aaccattgtg gcggttcgca gtttgtcccg gtacccagcg ttagtacgtc tagcgtcagc
    13801 tgtaaaattg tccagccgtt ttccagcaca tcgttttgct gcacccgtcg ccatttcttt
    13861 tgcccgtctt ttattacacc gatgatgcgc tcgcctcgcc ctaccatccc ctgatagcgc
    13921 cactggctaa gttcgctaat ccggcataga tcttccggcg gtttaaaagg gtcacgcata
    13981 ccggttaaaa ggcacaatgc aatacctgcc aacaaccagc gtttaaccct catgcggcgt
    14041 ctccagttgt agggtgaaca aaagatcatc accttccacg cttaatgaaa aacggctcac
    14101 gctgacgttg cgctctgcca gccgtgtaaa tgccgacggc actgcttccc acagcgtttt
    14161 caacgccaac tcgcctccct gcgcggatgg atgccagaaa accagttgtg cgccgggcaa
    14221 ctgaaaatcc agtggcgaaa agggcagcgt tttttcttcg ctgaagggag tggtgtcaac
    14281 caggcgatac agtgtggccc aatgctgatg atgacttgcc cgtagtcgaa ttaatgcgtc
    14341 gcgctcttca tggtgtgtcg atgacagaaa aatgagcgta actaacatca gcaaccagaa
    14401 tgcccagcaa aactggcgga ggcggggtga tgtggcgaac caccagtcaa agaacatgtt
    14461 catcgctaac cttccttgtt aactgatact caaattgcca gcgtccctgc gcatcctgct
    14521 gcgtggcccc ccgctgattg agatgaaaag atgcatcctg gcggagtgac gtttctagtg
    14581 cgtttaacgc ggtaatgctt gttgtaagcc ccttgatctc cagcgttccc tgctgccagc
    14641 ttatcgttgt cagccaggcg tgctcgggta aaagcgccgc cagtgcttcc agcgcagatt
    14701 gccagtcgcg ggtaaattgt cgctggcgct gcctttgcaa acgctgctca cgaatttgtt
    14761 ggcgctccag caaacgtggt ttggttatct gtaagctgcg ggcgagttgt tgttccccct
    14821 gaagcaaaac ggcgtctacg cgagcttcgg cgctgcttgt cagacgcagt attagcgtta
    14881 tcccgacggc cagcagcaga ggcgcaacga acatcagcaa ccagaaacga agaaaagcgg
    14941 tccggcgttg ctgtcgccag ggcaaaaaat taattggcgg gttcatcagt acgccttccc
    15001 aagcgccagc ccagcgcgat ggcaaagtct ccacctggcg gcggtaacgg cggctgacga
    15061 acagaaacgg cctcccaagg atcaaatccg ccttcgccac atatcgcgac gctttcggga
    15121 tcaacggata acgctgccgc cagctccttc gcgctagtca tccccaccgc cagtttgcgc
    15181 ccccagcgat agcgcgtcgc ccacagccac tgttcgttat cacgccaggc cagacattgc
    15241 tgatgagaag gtaaaaaagg caggaatcgc tgtaatgcac tggcatccgg ggtgatcgca
    15301 ctaacatgaa cacgcaacct ttctgccaaa gtaagcagcg ttgccagctc tttgctttgc
    15361 gcggcagtca cgttataggc gggactgagt gagtcttcgc tgtaatcgaa gcgcagggag
    15421 tccggatcca tatccagctc gcgagccatc gtcccggaca gccaggccgt ttgctcccgc
    15481 tcaccgaggg acatcgacgg gcgcggaaat gaccgctgta atgtgcgact ggcgggaaac
    15541 gccaacatga tgtgatggcg ctgcggcagt tcgcgactcc acggtaacaa cgttttagcc
    15601 agccgctgcg catcaacaat ccgcccatct ttgataatgt cgtgcgccag cggcaaccgc
    15661 caccagcgtt gcaaaaagca ttcttttgcg ccccgcacaa tagcaaccgc taccgcttct
    15721 tgctgttgta aatgcaaacc aatttgccag atcttaaatg ccattgtgat gatctcctta
    15781 tcacccgtca ctctgacggg tatatcaatg cgtctggctt gcctttatac taccgcgcgt
    15841 ttgtttataa actgcccaaa tgaaactaaa tgggaaattt ccagtgaagt tcgtaaagta
    15901 ttttttgatc cttgcagtct gttgcattct gctgggagca ggctcgattt atggcctata
    15961 ccgctacatc gagccacaac tgccggatgt ggcgacatta aaagatgttc gcctgcaaat
    16021 tccgatgcag atttacagcg ccgatggcga gctgattgct caatacggtg agaaacgtcg
    16081 tattccggtt acgttggatc aaatcccgcc ggagatggtg aaagccttta tcgcgacgga
    16141 agacagccgc ttctacgagc atcacggcgt tgacccggtg gggatcttcc gtgcggcaag
    16201 tgtggcgctg ttctccggcc acgcgtcaca aggggcaagt accattaccc agcagctggc
    16261 gagaaacttc ttcctcagtc cagaacgcac gctgatgcgt aagattaagg aagtcttcct
    16321 cgcgattcgc attgaacagt tgctgacgaa agacgagatc ctcgagcttt atctgaacaa
    16381 gatttacctt ggttaccgcg cctatggtgt cggtgctgcg gcacaagtct atttcggaaa
    16441 aacggtcgac caactgacgc tgaacgaaat ggcggtgata gccgggctgc cgaaagcgcc
    16501 ttccaccttc aacccgctct actcgatgga tcgtgccgtc gcgcggcgta acgtcgtgct
    16561 atcgcggatg ctggatgaag gatatatcac ccagcaacag ttcgatcaga cacgcactga
    16621 ggcgattaac gctaactatc acgcgccgga gattgctttt tctgcgccgt acctgagcga
    16681 aatggtgcgc caggagatgt ataaccgtta tggcgaaagt gcctatgaag acggttatcg
    16741 catttacacc accatcaccc gcaaagtgca gcaggccgcg cagcaggcgg tacgtaataa
    16801 cgtgctggac tacgacatgc gccacggcta tcgcggcccg gcaaatgtgc tgtggaaagt
    16861 gggcgagtcg gcgtgggata acaacaagat taccgatacg ctgaaggcgc tgccgaccta
    16921 tggtccgctg ctgcctgccg cagtcaccag cgccaatcct caggaagcga cggcaatgct
    16981 ggcggacggg tcgaccgtcg cattgagtat ggaaggcgtt cgctgggcgc gtccttaccg
    17041 ttcggatact cagcagggac cgacgccgcg taaagtgacc gatgttctgc aaacgggtca
    17101 gcaaatctgg gttcgtcagg ttggcgatgc atggtggctg gcacaagtgc cggaagtgaa
    17161 ctcggcactg gtgtcgatca atccgcaaaa tggtgccgtt atggcgctgg tcggtggctt
    17221 tgatttcaat cagagcaagt ttaaccgcgc cacccaggca ctgcgtcagg tgggttccaa
    17281 catcaaaccg ttcctctaca ccgcggcgat ggataaaggt ctgacgctgg caagtatgtt
    17341 gaacgatgtg ccaatttctc gctgggacgc aggtgccggt tctgactggc agccgaaaaa
    17401 ctcaccaccg cagtatgccg gtccaattcg cttacgtcag gggctgggtc agtcgaaaaa
    17461 cgtggtgatg gtacgcgcaa tgcgggcgat gggcgtcgac tacgctgcag aatatctgca
    17521 acgcttcggc ttcccggcgc aaaacattgt ccacaccgaa tcgctggcgc tgggttcagc
    17581 gtccttcacc ccaatgcagg tggcgcgcgg ctacgcggtc atggcgaacg gcggcttcct
    17641 ggtggacccg tggtttatca gcaaaattga aaacgatcag ggcggcgtga ttttcgaagc
    17701 gaaaccgaaa gtagcctgcc cggaatgcga tattccggtg atttacggtg atacgcagaa
    17761 atcgaacgtg ctggaaaata acgatgttga agatgtcgct atctcccgcg agcagcagaa
    17821 tgtttctgta ccaatgccgc agctggagca ggcaaatcag gcgttagtgg cgaagactgg
    17881 cgcgcaggag tacgcaccgc acgtcatcaa cactccgctg gcattcctga ttaagagtgc
    17941 tttgaacacc aatatctttg gtgagccagg ctggcagggt accggctggc gtgcaggtcg
    18001 tgatttgcag cgtcgcgata tcggcgggaa aactggtacc actaacagtt cgaaagatgc
    18061 gtggttctcg ggttacggac cgggcgttgt gacctcggtc tggattggct ttgatgatca
    18121 ccgtcgtaat ctcggtcata caacggcttc cggagcgatt aaagatcaga tttcaggtta
    18181 cgaaggcggt gccaagagtg cccagcctgc atgggacgct tatatgaaag ccgttcttga
    18241 aggtgtgccg gagcagccgc tgacgccgcc gccgggtatt gtgacggtga atatcgatcg
    18301 cagcaccggg cagttagcta atggtggcaa cagccgcgaa gagtatttca tcgaaggtac
    18361 gcagccgaca caacaggcgg tgcacgaggt gggaacgacc attatcgata atggcgaggc
    18421 acaggaattg ttctgattaa aaaggcgctt cggcgccttt tcagtttgct gacaaagtgc
    18481 acttgtttat gccggatacg ggtgaacgcg ttatccggct aacaaaatcg tgaaaactca
    18541 atatattgca ggaaccccat aagcctgata agcattgtgc atcaggcaaa cttcacgcat
    18601 ttacactcgc ccctgccctt tcaaccattc gcgcacgagg aacagcgcgc tgacattacg
    18661 cgcttcgttg aagtcagggt cttccagcaa atccatcata tgcgccagcg gccagcgcac
    18721 ctgtggtagc ggctctggct catcgccttc cagcgattcc gggtagagat cttgcgctac
    18781 cacgatattc attttgctgg aaaagtaaga cggtgccatg ctgagcttct tcaaaaaagt
    18841 cagatcgttc gcaccaaatc caacctcttc ttttagctcg cggttagcgg cttcgaagac
    18901 gctttcaccc ggatcaatta atcctttcga aaaacctaat tcgtaggatt cagttcccac
    18961 tgcatattcg cggatcagga tcaggtgatc gtccacaatc ggcacaatca tcactgcttc
    19021 ccggttggtt ggacgcattc gttcataaac acgccgcacg ccattgctga actctagatc
    19081 cacgctctcg acggtaaaca gtcgggaacg ggctacagtt tcaacattca gaatggtggg
    19141 tttttgtaat gatttgctca tcgtgggatc tatgctgtga aatcagcggt tattgtgcga
    19201 tatcggacac gctttcggca atgtgaattg catgttattt acatttatgt aacttaataa
    19261 ataatcgtcc tcaaatcaaa ttaaaagtca ataggttgaa ataactccag gaatttgctg
    19321 atattccgcc ttcagagggt ttgctatgat cagcggttac tgtgatgtgc ttaatgatgc
    19381 tcaagttaaa ctccacgctt gccgatagcc aaccgcagaa tcatgtattg tgtccggtgc
    19441 gactgaccac gcctgacaga ctaagtaaga tggggaaagc atgagcacca ttgtgatttt
    19501 tttagctgct ttgctggcct gctcactact tgcgggatgg ctgataaaag tgcgatccag
    19561 acggcgtcag ctgccctgga ccaacgcctt cgcggatgcg caaacgcgta aactcacacc
    19621 tgaagaacgt agcgccgttg aaaattatct tgagagcctg acgcaggtat tacaggtgcc
    19681 tggcccaacg ggagccagcg cggcaccgat ctctctggcg ctgaatgccg aaagcaacaa
    19741 cgtcatgatg ctgacacacg ctatcacgcg ttacggcatc tctaccgacg atccgaataa
    19801 atggcgttac tacctcgatt cggtagaagt ccacctgccc cctttctggg agcagtacat
    19861 caacgatgag aataccgttg aactgattca taccgattcg ctgccgctgg ttatttcact
    19921 caacggtcat acgctgcagg agtacatgca ggaaactcgc ggctatgcct tgcaacctgt
    19981 tccgtccacg caggcgtcga ttcgcgggga agagagtgag caaatcgagc tactcaatat
    20041 tcgcaaagaa acgcatgaag aatatgcgct gagtcgtccg cgcgggctgc gtgaagcgtt
    20101 gctgatcgtc gcctccttcc tgatgttctt tttctgcctg attaccccgg atgtatttgt
    20161 tccgtggctg gcaggcggcg cgttactgct gctgggcgca ggtctgtggg ggctattcgc
    20221 ccccccggca aaatcctccc tgcgggaaat tcattgtctg cgcggtacac cccgtcgttg
    20281 gggattgttt ggcgaaaacg atcaggaaca gatcaacaat atttcactcg gtattatcga
    20341 cctggtctat cccgcacact ggcagccata cattgctcag gatctcggtc aacaaaccga
    20401 tattgatatc tatctcgacc gccacctagt gcgtcagggg cgatatcttt cgctgcatga
    20461 tgaagtaaaa aactttccgt tacagcactg gctgcgcagt acgattatcg ctgcgggctc
    20521 gctgctggtg ctgtttatgc tgttattctg gatcccgctg gatatgccgc tgaaattcac
    20581 tctctcatgg atgaaaggtg cgcagaccat tgaagccacc agcgtaaaac aactggctga
    20641 cgctggcgtg cgggtaggcg atacattgcg tattagcggt acgggaatgt gtaatattcg
    20701 cacttccgga acctggagcg cgaaaaccaa ttcacctttt ttaccgtttg actgctcgca
    20761 gatcatctgg aacgacgccc gctcattacc attaccagaa tctgaactgg tcaacaaagc
    20821 gacggcattg actgaagcag ttaatcgcca gctgcaccct aaaccggaag atgaatctcg
    20881 cgtcagcgcc tcattacgtt cagcaattca aaaatccggc atggtattgc ttgatgattt
    20941 tggcgacatt gtactgaaga cagcggatct gtgttctgcc aaagatgact gtgtgcgact
    21001 gaaaaatgcg ctggtcaatc tcggcaacag taaagactgg gacgcgctgg taaaacgcgc
    21061 caacgccggg aagctcgatg gcgtgaatgt gttattacgc ccggtgagtg cggaatcgct
    21121 ggataacctg gtggcaacct ccaccgcgcc gtttatcacg catgaaacgg cgcgagcggc
    21181 acaatcacta aacagtccgg cccccggcgg attcctgatt gtcagcgacg aaggcagcga
    21241 ttttgttgat cagccctggc cttcggcatc actttacgac tacccgccgc aagaacagtg
    21301 gaacgctttc cagaaactgg cacaaatgct gatgcatacg ccgtttaacg ccgagggtat
    21361 cgtcacaaaa atcttcactg acgccaatgg tacgcagcat ataggccttc atccgatccc
    21421 ggatcgttcc ggcctgtggc gctatctcag caccacgctg ctgctactga cgatgctggg
    21481 tagcgccatt tataatggcg tacaggcctg gcggcgttac cagcgtcatc gcactcgcat
    21541 gatgaagatt caggcctatt atgaaagctg cctgaacccg caactgatca ccccttcaga
    21601 aagccttatc gaataacacg tttgcgcggc aggttatgct accctgtcgc gcaaattgct
    21661 tcactctgga gattttcctc atgcatatca acattgcctg gcaggacgta gataccgttc
    21721 tgctggatat ggacggcacg ttgctcgacc tcgccttcga taactatttc tggcaaaagc
    21781 tggtgcctga aacatggggc gcgaaaaacg gggttacgcc acaggaagcg atggaatata
    21841 tgcgccagca atatcacgac gtacagcata cgctaaactg gtactgtctt gattactgga
    21901 gtgagcaact gggtctggat atctgtgcga tgaccaccga gatgggaccg cgtgccgtac
    21961 tgcgtgaaga taccattccg tttcttgagg cactgaaagc cagcggtaag cagcgaattt
    22021 tgctcaccaa tgcgcatccg cacaacctgg cggtaaaact tgagcatacc ggtctggacg
    22081 cacaccttga tttattactt tccacccaca catttggtta tccgaaagag gatcagcggt
    22141 tatggcatgc ggtggccgaa gctacgggtc tgaaagctga aagaacgctg tttattgatg
    22201 acagcgaagc gattctcgat gctgccgcgc aatttggtat tcgttactgc ctcggcgtga
    22261 ctaatcctga ctcagggatt gccgagaaac agtatcaacg ccatccgtca ctgaatgact
    22321 accgccgcct gatcccctcg ctaatgtgaa ggagacgcca tgaaagaaaa acctgctgtt
    22381 gaggttcgac tggataaatg gctatgggct gcccgttttt ataaaacccg cgcgctggcc
    22441 cgtgaaatga ttgaaggcgg taaggtgcat tacaacgggc agcgcagcaa gccgagcaaa
    22501 atcgtcgagc tgaatgccac gctcactctg cgccagggaa atgacgaacg cacggtgatt
    22561 gtaaaggcga ttactgaaca gcgtcgcccc gccagcgagg cagccttgct gtatgaagag
    22621 actgcggaaa gtgtagagaa acgcgaaaaa atggcgctgg cacgtaaact taatgcctta
    22681 accatgccgc acccggaccg acgcccggac aaaaaagagc gccgcgacct gttacgattt
    22741 aaacacggcg acagtgaata actgtcacct gcaagagaga tgattatgcc gcaacatgac
    22801 caattacatc gctatctgtt tgaaaacttt gccgtgcgcg gcgaactggt aaccgtttcg
    22861 gaaaccctgc aacagatcct tgagaatcac gattatccgc agcccgttaa aaacgtgctg
    22921 gcagaactgc tggttgcgac cagcctgtta accgctacgc tgaagtttga tggtgatatc
    22981 accgtacagc tgcaaggcga cggtccgatg aatctggcgg ttattaacgg taacaacaac
    23041 cagcagatgc gcggtgtggc gcgcgtgcag ggcgaaattc cagaaaatgc cgacctgaaa
    23101 acgctggtcg gcaatggtta cgtggtgatc accattaccc cgagcgaagg tgaacgctat
    23161 cagggcgtgg tgggtctgga aggtgatacc ctggcggcct gcctggaaga ttactttatg
    23221 cgttctgaac agctgccgac gcgcctgttt attcgcaccg gcgacgtaga cggcaaaccg
    23281 gctgcaggcg gtatgttgtt gcaggtaatg cctgcgcaaa atgcccagca ggacgacttt
    23341 gaccacctgg cgacgctaac cgaaaccatc aaaaccgaag aactgctgac cttaccggca
    23401 aacgaagtgt tgtggcgttt gtatcacgaa gaagaggtga cggtttacga tccgcaggat
    23461 gtggagttca aatgcacctg ctcgcgtgaa cgttgcgccg atgcgctgaa aacgctacct
    23521 gatgaagaag tcgatagcat cctggcggaa gatggcgaaa ttgacatgca ttgtgattac
    23581 tgcggtaacc actatctgtt caatgcgatg gatattgctg aaatccgcaa caacgcgtct
    23641 ccggcagatc cgcaagttca ttaatgagta tgtccggcag agagagctct gccggatttt
    23701 acgactaata cacctgatgc gctttgtcac tcattcagat tcacatccag cctttcacca
    23761 tccactacaa gatcgcgcca cgctttaggc ttcgttccca gttttccatc cttcgcagct
    23821 gtgagtaatt tttctttcgt tatatcacgc ggaagtaaac tcatcgtaag ctcttgccat
    23881 tctttcttgt caggatcaaa gccatagaca atgtatcttt catcatcaaa ggcaaagagg
    23941 atccgctctg ccctgccatc actattcaga tcttgctcga ccagtacgca ggcgtctttt
    24001 tcaatacagg tcatcacgtt atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc
    24061 ggtttaccag aaccaggggc aattaacaca ttttcggcta atactttttc cgatatctgt
    24121 tgctgaagat gttgctctcc atctaacgcc atcagtagat cccgcgcgcg tttcgggtct
    24181 ttcatatact cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgaccgctg
    24241 tgttcgagca tgtagatagt tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc
    24301 atatgactgt tcacactaat gcgcatactg tccagcaccg gcgaattaag aagcaccagt
    24361 attaccaaca ccaataacga aaccgcaagg ttcactttgc cctgaagaac aaggggattt
    24421 tgccctttac gccagacgat actgacaaaa taccccagcg accataccag taacaccagc
    24481 accgccagcg cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat
    24541 aacgcccagg cggcgatgaa cacatacagc ggagcaacca gcagagcggt tttaatcagg
    24601 caacgcaacg gccccgtcca gggaagtgac gctttttgcg gatcgcggac aatagccatt
    24661 aagatcaatt gcaaaaaggc cagcgttaac agcaacccgg cggcggagat gtggcgagaa
    24721 atcgcactca ggcctgtaaa cggcagggtg atgataaaca tcagggttag caatgatact
    24781 aacggcagca accccgtggc gattaacgtg aacaactttt gaatagagtc gattaaacgt
    24841 gactgtgttc gcgcgaggat caccgccagc gcggtaacca gacctaacgt gagataaata
    24901 aaccagtcgg ttgcaaaaaa gagcgttttg aaaaacgtga tgccaacaag tttaaacaac
    24961 tcactccaca gtaaaagcac cagccacgtc agaccattgg cgagaaaaat caccagtaat
    25021 ataagtacgt tatgccatac tgactggtaa aaatagcgat agcggctact gtcattgcgg
    25081 atgcgaaggc tttgttgtat ccacggtagc aacaacattg ccatcagcag cagatagcaa
    25141 ccaaaatccc aaagcgcctt ttcagctctc cagggggtca tgccatcagt ttgccacttc
    25201 agccagccgc tcatccccag cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc
    25261 ttctgtttaa aagagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc
    25321 acgccataaa caatccaact gtgatttttt ccggaaagcc aggcgatcag caggtagcaa
    25381 accagaccct gaagtaatcc ggtagcaatc atcccccagc gtgttgcggg tgaaagttca
    25441 acgttatcca tgttggttat ccagaatcaa aaggtgggtt aattatcgca tccgggcagt
    25501 agtattttgc ttttttcaga aaataatcaa aaaaagttag cgtggtgaat cgatacttta
    25561 ccggttgaat ttgcatcaat ttcattcagg aatgcgattc cactcacaat attcccgcca
    25621 tataaaccaa gatttaacct tttgagaaca ttttccacac ctaaaatgct atttctgcga
    25681 taatagcaac cgtttcgtga caggaatcac ggagtttttt gtcaaatatg aatttctcca
    25741 gatacgtaaa tctatgagcc ttgtcgcggt taacaccccc aaaaagactt tactattcag
    25801 gcaatacata ttggctaagg agcagtgaaa tgcgcgttaa caatggtttg accccgcaag
    25861 aactcgaggc ttatggtatc agtgacgtac atgatatcgt ttacaaccca agctacgacc
    25921 tgctgtatca ggaagagctc gatccgagcc tgacaggtta tgagcgcggg gtgttaacta
    25981 atctgggtgc cgttgccgtc gataccggga tcttcaccgg tcgttcacca aaagataagt
    26041 atatcgtccg tgacgatacc actcgcgata ctttctggtg ggcagacaaa ggcaaaggta
    26101 agaacgataa caaacctctc tctccggaaa cctggcaaca tctgaaaggc ctggtgacca
    26161 aacagctttc cggcaaacgt ctgttcgttg tcgacgcctt ctgtggtgcg aacccggata
    26221 ctcgtctttc cgtccgtttc atcaccgaag tggcctggca ggcgcatttt gtcaaaaaca
    26281 tgtttattcg cccgagcgat gaagagttgg caggtttcaa accagacttt atcgttatga
    26341 acggcgcgaa gtgcactaac ccgcagtgga aagaacaggg tctgaattcc gaaaacttcg
    26401 tggcttttaa cctgaccgag cgcatgcagc tgattggcgg cacctggtat ggcggcgaaa
    26461 tgaagaaagg gatgttctcg atgatgaact acctgctgcc gctgaaaggt atcgcttcta
    26521 tgcactgctc cgccaacgtt agcgagaaag gcgatgttgc ggtgttcttc ggcctttccg
    26581 gcaccggtaa aaccaccctt tccaccgacc cgaaacgtcg cctgattggc gatgacgaac
    26641 acggctggga cgatgacggc gtgtttaact tcgaaggcgg ctgctacgca aaaactatca
    26701 agctgtcgaa agaagcggaa cctgaaatct acaacgctat ccgtcgcgat gcgctgctgg
    26761 aaaacgtcac cgtgcgtgaa gatggcacta tcgactttga tgatggttca aaaaccgaga
    26821 acacccgcgt ttcttatccg atctatcaca tcgataacat cgttaagccg gtttcaaaag
    26881 cgggtcacgc aacgaaagtt atcttcctga ccgcagacgc ctttggtgtg ctgccaccgg
    26941 tttctcgtct gactgccgat caaacccagt accacttcct ctctggcttc accgccaaac
    27001 tggccggtac tgagcgtggc atcaccgaac cgacgccaac cttctccgct tgcttcggcg
    27061 cggcattcct gtcgctgcac ccgactcagt acgcagaagt gctggtgaaa cgtatgcagg
    27121 cggcgggcgc gcaggcttat ctggttaaca ctggctggaa cggcactggc aaacgtatct
    27181 cgattaaaga tacccgcgcc attatcgacg ccatcctcaa cggttcgctg gataatgcag
    27241 aaaccttcac tctgccgatg tttaacctgg cgatcccaac cgaactgccg ggcgtagaca
    27301 cgaagattct cgatccgcgt aacacctacg cttccccgga acagtggcag gaaaaagccg
    27361 aaaccctggc gaaactgttt atcgacaact ttgataaata caccgacacc cctgcgggtg
    27421 ccgcgctggt agcggctggt ccgaaactgt aatgatttga agctggagaa tatctatcca
    27481 gtatcttata gaaagcaaaa cgggaggcac cttcgcctcc cgtttattta cccttctttt
    27541 gtcgtgctct gcgcccgcgt taccggcaca ggcagccagg cgcgaatgga aagcccgccc
    27601 cgctcgctgg tgccaagctc cagcatcccg ttatggttat ccacgatacg ctgcacaatc
    27661 gccagcccta atcccgtgcc gctaatggtg cgtgcactgt cgccgcgaac aaacggctgg
    27721 aaaaggtgct tacgttgttc cggcgcaatt cccggaccgt catcttccac ctggaaccag
    27781 gcgcgattcg gctccgtccc gctgctgact ttgatccagc cattgccgta acgggcggcg
    27841 ttgaccacca tattcgccac cgcgcgtttg atcgacagcg ggtgcatttt cacttcaatg
    27901 ctgccggggt aaagcgcggt ttcaatttcc cgctcatagc cactttcggc agcaataacc
    27961 tcaccgagta ctgcgttgag atccgccatt tccatcggca tctcctgtcc ggtgcgcagg
    28021 tagtcgataa actgctcaat gatggcattg cactcctcga tatctttatt gatcgattct
    28081 gccaggtagc catcctgctc gctcatcatc tcggtcgcca ggcgaatacg cgtcagcggt
    28141 gtacgcaagt cgtgacttac ccccgccatc agcagcgtgc ggtcatccgc cagttgctta
    28201 acaccagccg ccatatggtt aaaggcgcgg gtaacggaac gcacctcgga cgcgccatac
    28261 tcacgcagcg gcggcggaat aatcccttta ccaacctgca aggctgcgtg ttcgagatcg
    28321 accaacggtc gattctggat acgaataaac agccacgccc cgcctatcgc caatagcata
    28381 atcgccaacg tatagcggaa cagcggagag aaatcgccct gatgaatttc ggtcagcggc
    28441 acgcgtaccc agatattggg cgacagccag gttttcagcc agacgacagg cgaacttttg
    28501 ttgacctcaa cgcgcacttc cgtcgggccg cccagttgct gtgccatctg atggcttaag
    28561 aattcatagt gttgcgccca acgcagacct gcctcttcgg cagcctcgtt ggagtagaga
    28621 gagatcccca gctcacggta gatctcccga cggaaagcgg gaggcacaac caactgcgtg
    28681 ccgtcctcca gttgcagttt gtcggtcatc aacatacgca cttcgtacgc gaggacttta
    28741 ttaaactgct ggaggctcgg caaaatcgcg aagttcagca ccaccagata agtcgtcacc
    28801 aggctggcga acagcaaggt gacgatgagc aataacgtac gggcaaatga acttcgtggc
    28861 gagaagcgca atcgcctcat gctttagagc cgtccggtac aaagacgtag cccaggcccc
    28921 aaacggtctg aatgtaacgc ggatgcgctg gatcttcttc caccatgcgg cgcagacgag
    28981 aaatctgcac gtcgatggag cgttccattg cggaatattc acgaccacgg gcaaggttca
    29041 tcagcttatc gcgggagagc ggctcacgcg gatggctgac cagtgccttc agtaccgcaa
    29101 actcaccgct ggtgagcggc atcggctcgt cttcgcggaa catttcgcgc gtaccgaggt
    29161 taagtttgaa cttaccgaaa gcaattaccg cctcttcctg tgacggtgcg cctggtagct
    29221 cgttcgcctg acgacgcagc accgcacgga tacgggccag cagttcacgc gggttaaacg
    29281 gttttggaat gtaatcgtca gcgccaatct ccaggccaac gatacggtcc acttcttccc
    29341 ctttcgccgt caccataatg atcggcatcg ggttgctctg actacgaaga cgtcggcaaa
    29401 tcgacaagcc atcttcacca ggtaacatta aatccagtac cataagatgg aaagattcac
    29461 gagtcagcag gcgatccatc tgttctgcat tagcgacgct tcgaacctgg aagccttgtt
    29521 cggtgagata acgttccagc agcgcacgca ggcgcatgtc gtcatcgacc accagaatct
    29581 tgtagttctc ttgcattgtt tgtactccca aaggttcgca acaatttgta agcgtgtatt
    29641 cttaaaaaag ctcacgttcg tcaccagcta aatctggtat gaatttcagc ctaaattgtt
    29701 acaaagcata ttaaacagca gcttaagtat acaatttatt cggcgaaaca ttattgattc
    29761 tgttgatatg atcacgttat acccaatgtg cgcattatca aacagacaaa gggaatcaac
    29821 gagatgaaaa cgcccctggt tacccgggaa gggtatgaaa aactcaaaca agagcttaat
    29881 tatctctggc gtgaagaacg cccggaggtc acaaaaaagg tgacctgggc cgcaagtctg
    29941 ggcgaccgca gcgaaaatgc tgactatcag tataataaaa agcgcctgcg tgaaatcgac
    30001 cgtcgcgtgc gctatctcac taaatgcatg gaaaatctca aaatcgtcga ttactccccg
    30061 cagcaggaag gcaaagtctt ttttggcgcg tgggtggaga ttgaaaacga cgatggcgtg
    30121 actcaccgtt tccgtattgt cggctacgat gaaatttttg gccgtaaaga ttacatctct
    30181 atcgattccc cgatggcccg cgcattgctg aaaaaagaag tcggcgatct ggctgtggtg
    30241 aatactcctg ccggggaagc gagctggtat gttaatgcta tcgagtacgt gaaaccgtaa
    30301 ggaagagtct taacctcctg ccgatggctg gcatttttgc cagccagtcc gtataactat
    30361 cccctgattt ttgatccgaa aagatgaact caaaccatga tgaatgattc gttctgccgc
    30421 attattgcgg gtgaaattca ggcgcgcccg gaacaggttg acgctgccgt tcgcctgctt
    30481 gacgaaggga ataccgtgcc ttttattgca cgttatcgta aggaaatcac cggcggtctg
    30541 gatgacacgc agctgcgtaa tctggaaacg cgtctgggat acctgcgcga gctggaagag
    30601 agacgtcagg cgatcctcaa gtccatttcc gagcaaggca aactcaccga tgatctggcg
    30661 aatgccatca acgccaccct aagcaaaacc gaactcgaag acctctacct gccctacaaa
    30721 cctaaacgcc gcacccgcgg gcaaatcgcc attgaagcag ggcttgagcc gttggctgac
    30781 ctgctgtgga gcgatccgtc acacacgcca gaagtcgccg ctgcgcaata tattgatgcc
    30841 gataaaggcg tggcagatac caaagccgcg ctggacggcg cgcgctatat cctgatggaa
    30901 cggtttgccg aagatgccgc gctgctggcg aaagtgcgtg attatctgtg gaagaacgcg
    30961 catttggttt ctacggtggt gagcggtaaa gaagaggaag gggcgaaatt ccgcgactat
    31021 ttcgatcatc acgaaccgtt gtccacggtg ccttctcacc gcgcgctggc gatgttccgt
    31081 gggcgtaacg aaggcgtact tcagctttcg ctgaatgccg atccacagtt cgatgagccg
    31141 cccaaagaga gctattgcga gcaaatcatc acggatcacc ttggcctgcg cctgaacaat
    31201 gccccggcgg atagctggcg caaaggcgtg gtgagctgga cgtggcgcat caaggtgctg
    31261 atgcatctgg aaaccgaact gatgggcacc gtgcgcgaac gtgcggaaaa tgaagcaatc
    31321 aacgtctttg cccgtaacct gcacgatctg ctgatggctg cccctgccgg actgcgtgca
    31381 acgatgggcc tcgatccggg tctgcgtacc ggggtaaaag tggcggtggt cgatgccact
    31441 ggcaaactgg tagcgaccga taccatttac ccgcacaccg gacaagccgc aaaagcagcg
    31501 atgactgttg ctgcgctgtg tgaaaagcat aacgttgaac tggtggcgat cggcaacggt
    31561 acagcttccc gcgaaaccga acgtttctat ctcgacgtgc agaagcagtt cccgaaagtg
    31621 accgcacaga aagtgatcgt cagcgaagct ggcgcgtcgg tttactcggc ttccgagctg
    31681 gcagcgcagg agttcccgga tctcgacgtt tcgctgcgtg gcgcggtttc tatcgcccgc
    31741 cgtttgcagg atccgctggc ggagctggtg aaaatcgatc cgaaatctat cggcgtaggt
    31801 cagtatcagc atgacgtcag ccagacgcaa ctggcccgca aactggatgc agtagtagaa
    31861 gactgcgtaa acgccgttgg cgtcgatctc aacaccgctt ctgttccgct attaactcgc
    31921 gtggcgggcc tgacgcgaat gatggcgcaa aacatcgtgg cctggcgcga tgagaacggc
    31981 cagttccaga accgtcagca actgctgaaa gtcagccgtc tggggccgaa agccttcgag
    32041 cagtgcgcgg gcttcctgcg cattaaccac ggtgacaacc cgctggacgc ctctaccgtt
    32101 cacccggaag cctatccggt ggtggaacgc attctggcag caacacagca ggcactgaaa
    32161 gatctgatgg gtaacagcag cgaactgcgt aacctgaaag cgtctgactt tactgatgaa
    32221 aaattcggtg tgccgacagt aactgacatc atcaaagagc tggaaaaacc gggtcgcgat
    32281 ccgcgtccgg aatttaaaac cgctcagttt gccgatggcg tcgagacaat gaacgacctg
    32341 caaccgggta tgatcctcga aggcgctgtc accaacgtca ccaactttgg cgcgtttgtc
    32401 gatatcggcg tgcatcagga cggcctggtt cacatctctt cattgtcgaa caagtttgtg
    32461 gaagatccgc ataccgtggt gaaagcgggc gacattgtga aggtgaaagt gctggaagtg
    32521 gatcttcagc gtaaacgtat cgccctgact atgcgtctgg acgagcagcc tggcgaaacc
    32581 aactctcgtc gcggcggcgg taacgatcgc ccgcaaaaca accgcccggc agccaaacca
    32641 cgcggtcgtg aagcgcagcc tgccggtaac agcgcgatga tggacgcgct ggcggcagca
    32701 atgggcaaaa aacgttaaac gcccggactg gcccctaaac ctgcgcgata aggcgttcac
    32761 gccgcatctg gcaacgaacg ccttgcctga cataaaagtg ccggagaata tctccggcat
    32821 atttattcca cagccaaact cacaatatat ttcgttaata tttatcactt cattaacaac
    32881 tgaaacctta attaaacatt agccagtcct ggtaattcgc tattcgaatt atattttcgc
    32941 tgcgatataa ccttgagcca catcaacatt gagtcagatt attattcaaa ccaacattcg
    33001 cacacatttt aagtattgct gatagaaacc attctcatta tcattgtact attgattatt
    33061 taatctctcc ttcgttggca aatcatctgg tctcatgtcg ctgtcaaacg ccccatgagg
    33121 tagttatcca gttaatgaga aacaagtagg cacctatgca atacactcca gatactgcgt
    33181 ggaaaatcac tggcttttcc cgtgaaatca gcccggcata tcgccaaaaa ctgctttctc
    33241 ttggcatgtt acctggctcc tcttttaatg tggtgcgcgt cgctccactc ggcgacccca
    33301 ttcatatcga aacccgtcgt gtgagcctgg tattacgcaa aaaagatctg gccttattag
    33361 aagtggaagc ggtttcctgt taatacggtg ataacaacaa tgaaaaaatt aaccataggc
    33421 ttaattggta atccaaattc tggcaagaca accttattta accagctcac tggcgcacgt
    33481 cagcgtgtag gtaactgggc tggcgttacc gtcgaacgta aagaagggca attctccacc
    33541 accgatcatc aggtcacgct ggtggacctg cccggcacct attctctgac caccatctca
    33601 tcgcagacct cgctcgatga gcaaatcgcc tgtcactaca ttttgagtgg cgacgccgac
    33661 ctgctgatta acgtggtgga tgcgtctaac cttgagcgta acctgtacct gacgctacaa
    33721 ctgctggaac tcggcattcc ctgcatcgtg gcactgaaca tgctcgacat tgccgagaag
    33781 caaaatattc gtattgaaat tgatgctctg tcggcgcgtc ttggctgccc agtgatcccg
    33841 ctggtttcaa cccgtggtcg cggtattgaa gcgctcaagc tggcgattga tcgctataaa
    33901 gctaacgaga atgtggaact ggtgcattac gcacagccgc tactcaacga agcagattca
    33961 ctggcaaaag tgatgccttc cgacatcccg ctgaaacaac gtcgctggct gggcctgcaa
    34021 atgctggaag gcgatatcta cagccgcgcc tacgccggtg aagcgtcgca gcatcttgat
    34081 gccgccctcg cccgtctgcg taatgagatg gacgatccgg cgctgcacat tgccgatgcg
    34141 cgttaccagt gcattgctgc catctgtgat gtggtaagca acaccctgac ggcagaaccc
    34201 agccgtttca ccactgcggt agataaaatc gtgctcaacc gtttcctcgg tctgccgatt
    34261 ttcctctttg tgatgtacct gatgttcctg ctggctatca acatcggcgg ggcgttacag
    34321 ccgctgtttg acgtcggctc cgtggctcta tttgtgcatg gtattcaatg gattggctac
    34381 acactccact tcccggactg gctgactatc ttcctcgccc agggcctggg tggcggtatt
    34441 aacaccgtgc tgccactggt gccgcagatc ggcatgatgt acctgttcct ctccttcctt
    34501 gaggactccg gctatatggc gcgtgcggcg tttgtgatgg accgcctgat gcaggcgctg
    34561 ggcttgccgg ggaaatcctt tgtgccgctg atcgtcggtt tcggttgtaa cgtaccgtcg
    34621 gtaatgggtg cacgtacgct tgatgcaccg cgtgaacgtc tgatgaccat catgatggca
    34681 ccgtttatgt cctgcggcgc gcgtctggct atcttcgcag tattcgcggc tgccttcttc
    34741 gggcagaacg gtgcgctggc ggtcttctcg ctgtatatgc tcggtatcgt gatggcggtg
    34801 ctgactggcc tgatgctcaa gtacaccatc atgcgcggtg aagcgacgcc gtttgtcatg
    34861 gagctgccgg tctaccatgt accacacgtt aaaagcctga ttatccagac ctggcagcgt
    34921 ctgaaaggct tcgttctgcg tgctggtaaa gtgattatca tcgtcagcat tttcctgagc
    34981 gctttcaaca gcttctcgtt gagcgggaaa atcgtcgata acatcaacga ctcggcgctg
    35041 gcttccgtca gccgggtgat caccccggtc ttcaagccga ttggcgtgca tgaagataac
    35101 tggcaggcaa cagttggcct gtttacgggt gcaatggcga aagaagtggt ggtgggtacg
    35161 ctcaacaccc tctacaccgc agaaaatatt caggacgaag agttcaatcc ggcggaattt
    35221 aacctcggtg aagagttgtt cagcgcggta gatgaaacct ggcagagcct gaaagacacc
    35281 ttcagcctta gcgtactgat gaaccccatt gaagctagca aaggcgacgg cgaaatgggt
    35341 accggggcga tgggcgtgat ggatcagaaa ttcggtagcg cagctgccgc atacagctac
    35401 ctgattttcg tcctgctgta tgtaccatgt atctcggtga tgggagccat cgcccgtgaa
    35461 tcaagccgtg gctggatggg cttctccatc ctgtgggggc tgaatatcgc ttactcactg
    35521 gcaacattgt tctatcaagt cgccagctac agtcagcacc caacttacag cctggtgtgc
    35581 attctggcgg ttatcctgtt taacatcgtg gttatcggtc tgctgcgccg cgcgcgtagc
    35641 cgggtggata tcgaactgct ggcaacccgc aagtcggtaa gtagttgctg cgcagccagc
    35701 accaccggtg attgccatta atggcttcac ttattcaggt gcgtgatttg ctggcgttac
    35761 ggggccgtat ggaagcggcc cagataagcc agacattgaa cactccacag ccaatgatta
    35821 acgccatgct gcaacaactg gaaagtatgg gcaaagccgt gcggattcag gaagaacctg
    35881 acggctgcct ctccggcagt tgtaaaagct gcccggaagg aaaagcctgt ctgcgcgagt
    35941 ggtgggcgct gcgttaacca tactccatcg cctgttttga aaagcccggt atgcgtttgc
    36001 atccgggctt ttttgcgtgc ggctttccat aaaaatgcaa ttcttgcagc gcgacgtaag
    36061 ttcctctgaa agcgtctcgc agggatgaaa actggctaat gcacaggtgt ggagtggcgc
    36121 gtagagtcgc ggcattcaaa caacaggtga aggaacgcca tgagcaaaaa gcagagttcc
    36181 accccacacg atgcgctgtt caaactcttt ttacgccaac cggagacggc acgcgatttt
    36241 cttgcgtttc atttaccagc acccattcac gcgctctgtg atatgaaaac cctcaagctg
    36301 gagtcgagca gctttattga tgacgatctg cgtgaaagct atcccgatgt gctgtggtcg
    36361 gtgaaaacag aacaaggacc aggatacatc tattgtctga ttgaacatca aagcacctca
    36421 aacaaactga tcgcatttcg catgatgcgt tacgctattg ccgcaatgca aaatcacctt
    36481 gatgccggat acaaaacgtt gccgatggtg gtgccattgt tgttttacca cggtattgaa
    36541 agcccctatc cctattcgct gtgttggctg gattgtttcg ccgatcccaa actggcaagg
    36601 cagctttatg cctccgcatt tccgctgatt gatatcaccg tcatgcctga tgatgaaatc
    36661 atgcagcacc gacgcatgac gctgctggag ttaattcaaa aacatattcg tcaacgcgac
    36721 ctgatggggc tggtagagca aatggcctgc ttattaagta gtggatacgc taatgacaga
    36781 caaatcaaag ggctgtttaa ttacatactg caaaccggcg atgcggtacg ttttaacgat
    36841 tttatcgacg gcgttgccga acgatcaccg aaacacaagg agagtttaat gactattgcg
    36901 gaaagattgc ggcaggaggg ggaacaatcc aaagccctgc atatagccaa aataatgctt
    36961 gaatccggag tccctcttgc agacatcatg cgctttaccg ggctgtcaga agaagagttg
    37021 gctgcggcga gtcagtaaag ttctgtctcg ccattcaaaa gtgcctacac cctctgcttc
    37081 aacgccacca gcacgtgacg aaactcgacc ggatgcgaaa taaacggcgc atgagccgct
    37141 ttggcgaaga tgtatgactc gctgtgtggc cagagtttat ccagcatcgg caccactttg
    37201 cgcggcacca gaccatcgag atagccatac agtcgcaaaa acggcatggg tacgttttgc
    37261 agcggcaagc ggagatcaac cgttttgagg atttccagcc caccattgag cacgtcaacc
    37321 tccggcatcg gtaacgccag aacggttttc ttcaacgccc gcgcatcctg acgcgccgtt
    37381 tcagtaccca tggtttgtaa cgcaaggaac cgttccaccg tacgctgaaa atcatcactg
    37441 agttgctgct gaaatcccgc cagcacgtcc ggttttatcc ccggccactc gtcacgagca
    37501 ctaaaacaag gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg atgggttaac
    37561 gcaatctggc ttgccaccag cccgcccaga ctccagccta accaaatggc tttatcaggt
    37621 gcctgtcgca gcacggcttc ggccatctcc gcaagtgaca gcgcaccaaa tccccggcta
    37681 cgcccgaagc cgggcaggtc aacaagatgc agcgtaaaat gcgagctaag ttcctcgtca
    37741 atgcaacgcc acacttcagc attcagtccc catccgtgca gcagcacaag atgaacattc
    37801 ccctgacctt tggtctgcca ccagatgtta ttcatccgct attgttctct tttgacttac
    37861 aaggatgaac atatgctaac agtaccggga ttatgctggc tatgccgaat gccactggca
    37921 ttaggtcatt gggggatttg ttcggtctgc tcacgcgccg cccgtacaga taaaacgtta
    37981 tgcccacaat gtggattacc cgccacacac tcccatcttc cctgcggtcg ctgcctgcaa
    38041 aaaccgccgc cctggcaaag actggtcacg gttgccgact atgcgccgcc gttaagtctg
    38101 ctcatccacc agcttaagtt ttcccggcgc agcgaaatcg ccagcgccct gtcacgtctg
    38161 ttactattgg aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc ggatcgtatc
    38221 atcagcgttc cgttatggca gcggcgtcac tggcgtcggg gatttaatca gagcgatttg
    38281 ctgtgtcagc cgttatcacg ctggttgcac tgccaatggg atagcgaagc catcacacgt
    38341 acacgggcca ctgcgaccca gcattttctt agcgcccggc tacgcaggcg caacctgaaa
    38401 aatgcctttc gccttgaatt gcctgtgcaa ggtcgccata tggtgattgt ggatgatgtc
    38461 gttaccaccg gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa tggtgcggcg
    38521 actgtccagg tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatgg gcgtattata
    38581 accaactaaa atagtcaact attaggccat tactatgatc cgtatttccg atgctgcaca
    38641 agcgcacttt gccaaactgc tggcaaatca ggaagaaggg acacaaatcc gcgtatttgt
    38701 gattaaccct ggcacgccta acgctgaatg tggtgtttct tattgtccgc cggatgccgt
    38761 ggaagccacc gacaccgccc tgaaatttga cctgctgacc gcgtatgttg atgagttaag
    38821 cgcaccatac ctggaagatg cagagatcga ttttgttacc gaccagttgg gttctcagct
    38881 aacactgaaa gcgcctaacg ccaaaatgcg taaagtggca gacgatgcac cactgatgga
    38941 gcgcgtggag tatatgctgc agtcgcagat caacccacag cttgctggtc acggtggccg
    39001 cgtttcactg atggaaatca ccgaagacgg ttacgccatt ctgcaatttg gcggcggctg
    39061 taacggttgt tccatggtcg atgtgacgct gaaagaaggg atcgagaagc agctactaaa
    39121 cgaattcccg gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg gcgaacactc
    39181 ctactactaa gttatcccct cttttgtaga ttgcccgatg cgacgctaaa gcgtcatttc
    39241 gggcctacgt tgaacatgtg ccagacgttg gcgcagcatt gctttggcaa atccctccct
    39301 tcgtttcccc gcgataatat gaccaacctc tcataattta aatttacccc gctctggtga
    39361 ttctcaaacg ccagatgtta cccgtatcat tcacgtgggt accaaacata ctcctgacat
    39421 ctgactacaa taattagttt tagtgggtat cagtcgtggt gccgcaatat ctctgttccc
    39481 gattgggata attagagctt gtcgtcagaa aattgacgtt acccataaca aatgaaaggc
    39541 caggtaaatc atgccattag tcattgttgc tatcggtgta atcttgttat tactcctgat
    39601 gatccgcttc aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg cggttggatt
    39661 aatgcaaggg atgccgctgg ataaagttat tggctccatc aaagccggtg tcggcgggac
    39721 gctcggtagc cttgccctga tcatgggttt tggcgcaatg ctgggcaaaa tgctggcaga
    39781 ctgcggtggc gcacaacgta tcgccaccac gctgattgcc aaatttggta aaaaacacat
    39841 ccagtgggcg gtggtactga ccggttttac cgttggtttt gccctgttct atgaagtggg
    39901 ctttgtgctg atgctgccgc tggtgtttac catcgcggct tctgcgaata ttccactgct
    39961 gtatgttggt gtaccaatgg cagctgcact gtctgtgacc cacggcttcc tgccaccgca
    40021 tccgggtccg actgcgattg ccaccatttt caatgccgat atgggtaaaa ccctgctgta
    40081 cggtactatt ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgtt
    40141 gaaaggtatc gataagccaa ttccggaagg tctctacagc gcgaaaacct tcagcgaaga
    40201 agagatgccg agctttggcg tcagcgtctg gacctctctg gtgccggtag tactgatggc
    40261 gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga
    40321 gttcctcggt gacccggtaa tggctacgct gattgccgtg ctgattgcga tgttcacctt
    40381 tggtctgaac cgtggtcgtt caatggatca gattaacgac acgctggttt cttccatcaa
    40441 aatcattgcg atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc aggtgctggt
    40501 agacagcggc gtggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct
    40561 gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc
    40621 ggcaatcact gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccgga
    40681 gctgatggtt attgcggtgg gttccggtag tgtgattttc tctcatgtga acgatccggg
    40741 cttctggctg ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc
    40801 gatgctggaa accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt
    40861 gatttgataa cactgactgc cggatgcatc gcatccggca tcaaccgcac tctacttctt
    40921 cttcgctgca gctctgcgcc gtctgtccaa atccttcagc aacttgttca cgccatcatc
    40981 ggcaaacatc gtctcaagcg ttgcggaaag cttgcgtcgc cagtttttat actggtaact
    41041 ggtgccagga atattcaccg gttcggccat atccagccag tcttccggct gtagtcctaa
    41101 cagagcactg ttactgtcgg caatgtagcg ctgcaaacca cgattaagcg tcggcgtcat
    41161 cgacatcaac gatgccttat gcccggcacg ttttggcaga caaccatatt tatgcagtgc
    41221 atccagcagc ccttgcttcg ccagttcgcg atcctgatac agaccgcgca gtaccacttc
    41281 atccggatac agccccaggg ttttgcccag cgttagatcc ccgctctccc agtagccgcg
    41341 tagcgtaggc aggtcatgtg tcgccgcaac cgccatcgac tgctccggat acgctttcgg
    41401 tgcacggaac gtcttctcgt ggtcgttttc gaaatagagc actttgtagg agtacacacc
    41461 gctgctgcgc agcttaccga caatctctac cggtacggta ccgagatctt caccaatcac
    41521 catacagcga tgacgtttac tttcgagtgc cagaatcgag agcagatcat ccaccggata
    41581 gtgaacatac gcgccctgat ctgccgtctc gccatacggt atccaccaca aacgcagcat
    41641 cgacatcaca tggtcaattc gtaatgcgcc gcagttttgc atattggcac gcaacagctc
    41701 gataaacggt tcataagcac gcgcggtgat gatatgcggg tccattggcg gcaatcccca
    41761 gttctgcccc aacgggccga ggatatccgg cggcgcgcca accgacgctt tcaggcaata
    41821 cagttcacgg tcgcaccagg tttccgcccc accttccgct acgccaaccg ccagatcacg
    41881 atacaagcca atcggcattt catagccctg gcttatctcc cagcaggcgg caaactggct
    41941 gtaagcctgc cactgcaacc agagataaaa atcaacgtca tcgcgatgtt cttcgcagaa
    42001 ctgacgcact tctggtgaat ccacgttctg atacatctct ggccatgcag gccagcccca
    42061 gcgcatttcg tcctctttca cttgctgggc atgtagcgca tcaaaggctg cctgccagaa
    42121 caggctgtcg ccttgatctg caacaaactg gcgaaacgcg gccatctgct catcatcacg
    42181 ttgcgcgaaa cctttccacg ccattcgtaa tgctgtcatt tttagggcgg taaccgtgga
    42241 gtaatcgacc cagtcggcat cccgcgcctg ttgcagcgtc tgttgcgtgg tcggcaactg
    42301 ccaccaggcc tgagcctctt cgctaagatg gaaatcttcg acggcgttaa cgtcgatata
    42361 aatcacattc agccagcggc gagaagacgg gctatacggg ctggcgctct ccggatttgc
    42421 cggatagagc gcatgaatcg ggttcagacc aatgaacgat ccgccacgtt ttgccacatc
    42481 cactagcatc gctttgagat cgccaaaatc cccaataccc cagttttttt ccgatcgcag
    42541 cgtataaagc tggacgcagg caccccacag cttttgttta ttcagcaacg cctgcggttc
    42601 gtaacagcgt ttcggggcga caatcaccct gcaatgcccg cgctggtctt cctgggtaag
    42661 cgtcagcgtg tgataacctt ccggcagctt cgtcggtaga ttgaacgctt tgccccccgt
    42721 tatatggcct ttgtactgcg ttccttcttc ggtggtcagc agccagctat attcgccgct
    42781 gccctccacc accatcggca tttttttgcc gctggtataa accatgacat tcgggactgg
    42841 cgttaccgcc actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac gccgtttggt
    42901 ttcggcgcta atcgactgcg gtttaccgtg ggcattgatg taatgagggc taatccccgc
    42961 cgccagcgcg gcattatcca gacgtttgct ttccatcgag cttccttagc gttttgcctg
    43021 ccagatacga gcctgataat cgcgaataga gcgatccgag ctaaacatac cgcagcgggc
    43081 ggtattgagg atcgccgcgc gagtccaggc ctcctggtcg cggtacagca catccacctg
    43141 cttttgtgcc tctacatagg ctgcgaaatc cgccatcacc agatacggat cgccgccctg
    43201 tttgccgata ctgtgcagca tctggtcgaa ggcatgctta tcgccgtcgc tgtatttacc
    43261 gctttccagc tctttcaata ctgcgtccag caccttatct ttcttccgcc atttcaccgg
    43321 gtcgtagcct ttggccagaa ttgccttcac ttgttccacg gtatgaccaa aaataaagat
    43381 attttcttca ccgactttct cggcgatttc aacgttcgcc ccatccagcg taccgacagt
    43441 aagcgcacca ttgagcgcca gtttcatatt gccggtaccg gaagcttctt tacctgcagt
    43501 cgaaatttgt tcggagatat ccgccgccgg gatcagtttt tccgccgccg aaacgcaata
    43561 atccggcagg aacaccacct tcaacttatc gccaaccagc ggatcgttgt tgatcacgtc
    43621 agccactttg ttgatcgcaa agataatatt tttcgccagg tagtagcccg gtgccgcttt
    43681 cgcgccgaag aggaagacgc gcggtacgcg atcagcctgt gggttttcac gaatttcttt
    43741 gtacaacgcc agaatatgca gcagattcag gtgctggcgt ttgtactcat gcagacgttt
    43801 gatctgaata tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa ctttcacaaa
    43861 ctccgccaga cggactttat tcgcctgctt gatctcgcga tattgctgac ggaatttcgc
    43921 atcatcagcg aatttttcca gattgatcag ctgatcgaga tcgttagccc actctttttt
    43981 cagtgattta tccaacagag ccgccagtgc cgggttgcac tgtttgatcc agcgacgtgg
    44041 ggtaataccg ttggtgacgt tatggaattt gttcggccat agctggtgat attccgggaa
    44101 cagatctttc accaccagat ccgagtgcag cgccgcaaca ccgttcaccg cgaaaccgcc
    44161 aaccacacac aggttcgcca tatgcacttg tttgtcgtgc accaccgcca gtttagccca
    44221 cactttttca tcgcccggcc aggttttctc caccagcgtt ttaaagcgag tattaatttc
    44281 gttaataatc tgcatgtggc gcggcagtaa gcctttcacc agtttcacat cccagcgttc
    44341 cagcgcttct ggcatcaggg tatggttggt gtaagcgaaa gttttgctgg taatggccca
    44401 ggcgtcatcc cagctcatct ggtgctcatc gatcagcacg cgcaacagtt ctggaatcgc
    44461 gatagttggg tgggtatcgt tcagctgaat aacttcgtaa tccgccagtt cgtgcagttt
    44521 acgccccgcc agatgatggc gacgcaaaat atccgctacc gaacaggcac actggaagta
    44581 ctgctgcatc aagcgcagct ttttaccggc agtatggttg tcgtttggat agagaacttt
    44641 ggtcagtttt tccgcattga tgccctgctg ttcggcacgc aggaaatcac cgtcgttaaa
    44701 tttagtcaga tcaaacggat gcgcgtgcgt cgcctgccac agacgcagcg gctgcgccac
    44761 gccattacga tagccgacaa cggggagatc ccacgcttga ccggtaatgg taaactccgg
    44821 ctcccagcgt ccgtctttcg tcactttacc gccaatccct acctgcacat ccagtgcttc
    44881 gttgtggcgg aaccacgggt agttactgcg atgccagtca tccggcgctt caacctgttt
    44941 gccatccaca aaagactggc ggaacaaacc atattgatag ttcagaccgt aacccgtcgc
    45001 agactgaccg acagttgcca ttgaatcgag gaagcacgcc gccagacgtc ccagaccacc
    45061 gttacccagc gcagggtcga tctcttcttc cagcaggtca gtcagattga tgtcataagc
    45121 cttcaacgaa tcctgtacat cctgatacca gccgagattc aacaggttgt tgcccgtcag
    45181 acgaccaatc aaaaactcca ttgagatgta gttaacatgt cgctgattcg ccaccggctt
    45241 ggcgaatggc tgagcacgca gcatttcggc cagtgcttca ctcactgcca gccaccactg
    45301 gcgaggagtc atttcagccg cagaatttaa gccataacgc tgccactgac gtgaaagcgc
    45361 ttcctgaaat tgcttatcgt taaaaatagg ttgtgacata ggagttccac ttttcttaga
    45421 ttttcaacac aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc tcatcctgcg
    45481 ggggattagg cagggaggag ttgcggggat gagcaaggaa atgtgatctc gaccacttaa
    45541 agctagtgca aaccacagga ttagcagcaa atcaatgcca tacagcgcag aaaatctgta
    45601 tctaagtgca aaaaatggcc gttgcgtatt ttcaaaaagc gaaaggtaac tctataaatt
    45661 aagtaaagga gtgaaacagt ttcacaagta aaatatccag tgtgctccat ctcattctta
    45721 atagatttat taagatcatc tttttagata gcactttcat caggaatgaa gaagaaaccc
    45781 ttgcttaaat gaatctgatg aacataaggg aaaccagtat tcacgctgga tcagcgtcgt
    45841 tttaggtgag ttgttaataa agatttggaa ttgtgacaca gtgcaaattc agacacataa
    45901 aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac cttataacca ttaattacga
    45961 agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa gtgattaact atgctgattc
    46021 cgtcaaaatt aagtcgtccg gttcgactcg accataccgt ggttcgtgag cgcctgctgg
    46081 ctaaactttc cggcgcgaac aacttccggc tggcgctgat cacaagtcct gcgggctacg
    46141 gtaagaccac gctcatttcc cagtgggcgg caggcaaaaa cgatatcggc tggtactcgc
    46201 tggacgaagg tgataaccag caagagcgtt tcgccagcta tctcattgcc gccgtgcaac
    46261 aggcaaccaa cggtcactgt gcgatatgtg agacgatggc gcaaaaacgg caatatgcca
    46321 gcctgacgtc actcttcgcc cagcttttca ttgagctggc ggaatggcat agcccacttt
    46381 atctggtcat cgatgactat catctgatca ctaatccagt gatccacgag tcaatgcgct
    46441 tctttattcg ccatcaacca gaaaatctca ccctggtggt gttgtcacgt aaccttccgc
    46501 aactgggcat tgccaatctg cgtgttcgtg atcaactgct ggaaattggc agtcagcaac
    46561 tggcatttac ccatcaggaa gcgaagcagt tttttgattg ccgtctgtca tcgccgattg
    46621 aagccgcaga aagcagtcgg atttgcgatg acgtttccgg ttgggcgacg gcactacagc
    46681 taatcgccct ctccgcccgg cagaataccc actcagccca taagtcggca cgccgcctgg
    46741 cgggaatcaa tgccagccat ctttcggatt atctggtcga tgaggttttg gataacgtcg
    46801 atctcgcaac gcgccatttt ctgttgaaaa gcgccatttt gcgctcaatg aacgatgcac
    46861 tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg cctcgaagag attgagcgtc
    46921 aggggctgtt tttacagcgg atggatgata ccggcgagtg gttctgctat cacccgctgt
    46981 ttggtaactt cctgcgccag cgctgccagt gggaactggc ggcggagctg ccggaaatcc
    47041 accgtgccgc cgcagaaagc tggatggccc agggattccc cagcgaagcg attcatcatg
    47101 cgctggcggc aggcgatgcg ctgatgctgc gcgatattct gcttaatcac gcctggagtt
    47161 tgttcaacca tagcgaactg tcgctgctgg aagagtcgct taaggccctg ccgtgggaca
    47221 gtttgctgga aaatccgcag ttggtgttat tgcaggcgtg gctgatgcaa agccaacatc
    47281 gctacggcga agttaacacc ctgctggccc gtgctgaaca tgaaatcaag gacatcagag
    47341 aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca ggtggcgatt aacgatggta
    47401 atccggatga agcggaacgg ctggcaaaac tggcactgga agagctaccg ccgggctggt
    47461 tctatagccg cattgtggca acctcggtgc tgggtgaagt gctgcactgc aaaggcgaat
    47521 tgacccgctc actggcgcta atgcagcaaa ccgaacagat ggcacgccag cacgacgtct
    47581 ggcactacgc cttatggagt ttaattcagc aaagtgaaat tctgtttgcc caagggttcc
    47641 tgcaaaccgc gtgggaaacg caggaaaaag cgttccagct gatcaacgag cagcatctgg
    47701 aacagttgcc gatgcatgag tttctggtgc gcatccgagc acaactgtta tgggcctggg
    47761 cacggctgga tgaagccgaa gcgtcggcgc gtagcgggat tgaagtctta tcgtcttatc
    47821 agccacagca acagcttcag tgcctggcaa tgttgattca atgctcgctg gcccgtggtg
    47881 atttagataa cgcccgtagc cagctgaacc gtctggaaaa cctgctgggg aatggcaaat
    47941 atcacagcga ctggatctct aacgccaaca aagtccgggt gatttactgg caaatgaccg
    48001 gcgataaagc cgccgctgcc aactggttgc gtcatacggc taaaccggag tttgcgaaca
    48061 accacttcct gcaaggtcaa tggcgcaaca ttgcccgcgc acaaatcttg ctgggcgagt
    48121 ttgaacctgc ggaaattgtc ctcgaagaac tcaatgaaaa tgcccgcagt ctgcggttga
    48181 tgagcgatct caaccgtaac ctgttgctgc taaatcaact gtactggcag gccggacgta
    48241 aaagtgacgc ccagcgcgtg ttgctggacg cattaaaact ggcgaatcgc accggattta
    48301 tcagtcattt tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag ctgattcaac
    48361 tcaatacgct gccggaactg gaacagcatc gcgcacagcg tattctacga gaaatcaatc
    48421 aacatcatcg gcataaattc gcccatttcg atgagaattt cgttgaacgt ctgctaaatc
    48481 atccggaagt gccagaactg atccgcacca gcccgttgac ccaacgtgaa tggcaggtcc
    48541 tggggctgat ttactccggt tacagcaacg aacaaatagc gggtgaactg gaagtggcgg
    48601 caaccaccat caagacgcat atccgcaatc tgtaccaaaa actcggcgtc gcccaccgtc
    48661 aggctgccgt gcagcacgcg cagaaactgc tgaagatgat gggttatggg gtgtgagttt
    48721 gtatagcgtt tttatgtaca ctctttatac tgtatataaa atcgcactac aggctcatga
    48781 atatgaaacc ggaaagcaaa gaagcgccaa tcaatatcag agcgaaagcc tcacagcgtg
    48841 acctgattga tatggcagcg aatctggtcg caaaatcgcg cactgatttt atgctggatg
    48901 ccgcatgccg tgaagcgcaa gacatcttat tagatcaacg actattcatc cttgatgacg
    48961 agcaatacga tgcgtttctc gctgccctgg acgcccccat cactgctgag cgtcaggcca
    49021 aaattaacgc gttgatgaat cgtaaatcgc catgggaata acggctccta ctcccctaac
    49081 gtctgaacat aatctggctg acttttgctg ttccgatcac gggatgaatg aatggctaaa
    49141 aaagaaagcg ttaaaaaatc acagctccgg tctgtctcgt gtttacgtta tctgtattgc
    49201 caatacccgc caggtaatcg gctattactg cctttctaca ggcagtattc aacgtaattt
    49261 ggcccctgga gccatgcgac gtaatgctcc cgaatcgttg cctgttgtcg tattaggcag
    49321 actggcaatt gatcaagcct gggcaggtaa agggttgggc gtcgcattgt tgaaagatgc
    49381 agtctatcgc acaatgtcca tagctcaaca ggtaggggtt cgtgcgctca ttgttcatgc
    49441 cttagacgat tcagtacgca atttctatct gaagtatgcc tttgtacctt caccattcca
    49501 gtcacttacc ttactgtatc ccattactct ggagtaattt acctctaccg gatagtcaca
    49561 ctgatgagtc tgatccggga gggatttcaa tcagtgagtt tggttatcat cacccgcgtt
    49621 acgccatctg tttcagccag agtaaaccgc accggcaaga aacgctccac cacggcgata
    49681 ttggtcagca gatggcatga gggatgggcg accgtaaatt gtcccgcgcc cgccagcgcc
    49741 attggcaaca ctagctggtc agcaagatat tcccccactg ccgccgggct tgccaggtag
    49801 cgtttcacct ctttcaccaa ctgtgccgca accacctcgg cactgacgcg cttttcaccg
    49861 acgacaaaaa agcgttcggt gatattttca ctttcgactt caagcgagac ggtattaccc
    49921 ggcccctggt cgcgcggcag gttatgaata ttctgctcat gcagggaaaa acttgccgcc
    49981 agcgtagcga tttcacgctc agcgacatgc cgcggcacgc cagctaataa cacctctcca
    50041 cgcatctgca caatgttccc gcgctcgcca agttgcaagg tgttaaacga tgccaccggc
    50101 gagacttccg ttgccaccac gccgcctccg gcaggataaa aaccgtgacg taacagcgtg
    50161 gtttgctgat gaattcctat tttcgccagc agcggctcca acacccggcg gataaaatcc
    50221 gcaggcgggg ccgacgggtt atcggtaccg ccgctcactt caacacgcga aggtccatcg
    50281 gcaaaccaca gcgcgggcag caccgtttgc agcaccagcg tacaacttcc tgcgctaccg
    50341 atagcaaagc gataatcgcc gccacgcacg gtgccgggcc ggaagaccag acgctgcgac
    50401 cccagctccg cgccttccac cgtcgccccg caaatttccg tcgccgcctt taccgccgtc
    50461 agatgctggc gcaacagtcc cggttttgcc cgcccggcac gaatgccggt gatggtaaat
    50521 ggctggccgg ttatcatcga cagactcagc gccgagcgca tgatctgccc gccgccttcg
    50581 ccctgtgcgc catccagcgc aatcatcctt ttcatcattt atccttttac gcacaccacc
    50641 tgacgtaggg tgtagataac ttccaccaga tcgctttgtg ccgccatcac cgcatcaata
    50701 tctttatacg ccatcgggat ttcgtcgatc acttcggcat ctttacggca ttccacatgc
    50761 acggtggcac gaatttgatc ttccacgctg aacatttttt tcgctttagt acggctcata
    50821 acccgcccgg caccgtggct gcacgaacag aacgactctt catttcccag cccacggacg
    50881 ataaagcttt tcgctcccat cgaccctgga ataattccat attgaccagc acgcgcagac
    50941 accgcgcctt tacgcgtcac gtagatctct tcaccaaagt gctgttcttt ttgcacatag
    51001 ttgtggtgac agttgatctc ttccatcgcc agcgtttgtg gctgtctgac cgttttctgc
    51061 gtaatgctct gcaacgccgt tactacgttt tccatcatca catcgcggtt aaggctggca
    51121 aaaagctgcg cccaggccac ggctttcagg taatcatcaa agtattccgt accttccata
    51181 aagtacgcca gatcacgcga cggcaatgtt tcaagcgttt cctgcatctc tttttgcgcc
    51241 agatcgataa agtaagtccc gatggcatta ccaattccgc gtgaaccgga gtgcagcata
    51301 atccacacct ggtccgactc atcaaggcag atttcaataa agtggttacc cgttcccagc
    51361 gttcccaggt gtttatagtt gttagtgtta aggaaacgcg gatatttttg cgttaaccac
    51421 tgataaccgg cctccagctc agcccatttc gcatcgacgt taacaggcgg gttttcccat
    51481 gcgcctttat cacgtttaca acggccagtg gtacgcccgt gcggcaccgc agtttcaatc
    51541 gcctgacgca gctccgccag gttttccggc aggtcagccg ccgttaaagc ggtacgtagt
    51601 gcgttcattc cacagccaat atccacgccc accgccgccg gaataatcgc ccctttggtc
    51661 gggatcacgc taccaatggt ggaaccttta cccaggtgta catcaggcat taccgcaata
    51721 tgtttgaaaa taaacggcat cttcgccgta ttaataagtt gctgacgcgc atcggcttct
    51781 accggcacgc ctttggtcca catttttacc ggggcatttt cagtggtcag taattcgtaa
    51841 ttcattttgt ttttctcttt tcgttgtttg cggtcctgat aattgcaacc gtcgtgccag
    51901 aaaattaaat aagcagatta attttttaat tcattgtttt aaaaagatta tatctttacg
    51961 tccgtaaacg gagatttccc gcaaagccaa tttaccgata atgaaatatc gtcttttata
    52021 aggatatcta agatgcgtaa aacagtggct tttggctttg tcggaacagt gctggattat
    52081 gccgggcgcg gcagtcagcg ctggtcgaaa tggcgtccgt cactctgtat atgccagcaa
    52141 gaatcgttag tcatcgatcg actggaatta ttgcatgacg cccgctcgcg ctcgctattt
    52201 gaaacgctta aacgcgatat cgccagcgtt tcgccagaaa ccgaagtggt gagcgttgag
    52261 attgaactgc ataacccgtg ggatttcgaa gaggtttacg cctgcctgca tgatttcgcc
    52321 cgtggttacg agtttcagcc agaaaaagaa gactatttaa ttcacatcac caccggcacc
    52381 cacgtcgcgc agatttgctg gtttctgctg gctgaagcgc gttacctgcc cgcccggttg
    52441 atacaatctt cacctccgcg caaaaaagaa cagccgcgcg gcgcaggtga agtgacgatt
    52501 atcgatctcg atttaagcct ttataacgcc atcgccagcc gctttgccga ggaacgccag
    52561 caaacgcttg attttcttaa gtccggtatt gccacgcgta acccccactt caaccgcatg
    52621 attgagcaga ttgaaaaagt agcgattaaa tcccgcgcac cgatcctgct aaacggccca
    52681 accggtgcgg gcaagtcatt tctggcgcga cgtatatttg aattaaaaca ggcgcggcat
    52741 cagtttagcg gcgcgtttgt ggaagtgaac tgcgcaaccc tgcgcggcga taccgccatg
    52801 tcgacgctgt ttggtcatgt gaaaggcgcg tttaccgggg cacgggaatc tcgtgaagga
    52861 ttattacgca gcgccaacgg cggaatgttg tttcttgatg agattggcga actgggcgca
    52921 gacgaacagg caatgctgct gaaagccatt gaagaaaaaa ccttttatcc gtttggcagc
    52981 gatcgccagg tgagcagtga ttttcagctt atcgccggaa cggtgcgtga tttgcgccag
    53041 ctggttgccg atggcaaatt ccgcgaagat ctatacgcgc ggatcaatct ctggaccttc
    53101 accctgccgg ggctacgcca gcgtcaggaa gatattgaac ctaacctgga ttatgaagtg
    53161 gagcgccacg cttcactcac cggcgacagc gtgcgtttta acaccgaagc gcggcgcgcc
    53221 tggctggcct ttgcgacatc tccccaggca acatggcgcg gtaactttcg cgagctttct
    53281 gccagcgtca cgcgaatggc aacctttgcc tctagcggac gcattactct ggacgtggtt
    53341 gaagatgaga taaaccgtct gcgctataac tggcaggaga gccgcccctc cgcgcttacg
    53401 gcgttgctgg gcgcagaggc agaaaacatc gatctcttcg accgtatgca actggaacac
    53461 gttatcgcaa tctgccgcca ggcaaagtcg ctttccgcag ccggacgcca gctttttgac
    53521 gtttcgcgcc agggcaaagc cagcgtcaat gacgcggatc ggctacgcaa atacctggcg
    53581 cgttttggtc tgacgtggga agccgtgcag gatcagcaca gctccagttg aatatggtgg
    53641 tccgtcagca cctgcatcac gcttactggc ggcggggtgt cggtgtagac ggcatctacc
    53701 atgctgatgc tacccatatt gaccatcgcg ttacggccaa atttcgagtg atcgacaacc
    53761 agcataacgt ggcgcgagtt ctcaataatg gcgcgtttgg tgcgaacttc gtggtaatcg
    53821 aactccagca gcgaaccgtc gctatcgatg ccgcttatcc ccagaatgcc gaaatcaagg
    53881 cggaactggg agataaaatc gagcgtcgct tcgccaatga tcccgccatc gcggctgcgt
    53941 aattcgccac cggcgagaat aatacggaaa tcctctttta ccatcagcgt gttagcaacg
    54001 ttaagattgt tggtgacaat gcgcaaattg ctgtgattga gcagcgcgtg cgccaccgct
    54061 tccggcgtgg tgccgatatc gataaacagc gtcgagccat tggggatttg ctccgccact
    54121 ttgcgggcga tgcgctcttt ttcttcggtc tgggtggctt tacgatcgtg ccacggcgtg
    54181 ttaaccgaac tggaaggcag cgccgcaccg ccatgatggc gcaggatcag gttttgctcc
    54241 gccagctcat tgaggtcgcg gcgaatagtc tgcgggctga cggagaagtg ctccaccagc
    54301 tcctcggtac tgacataacc ctgctgttta accagttcga taataccgtt gtgacgttgt
    54361 gtttgtttca tttctaaatc cctggaatta ttttcgtttt cgcgcattga gcgaatcaac
    54421 aaaagccatc gctaaaccca cggctaaccc ggcgatgtgt gctccgttcg ccatcgacat
    54481 cccaaacaaa tcaaaccatc cggcgacaat ccagatcagc gcaaagataa ttaacccacg
    54541 ttgcaggtaa atgccacttt gcggatcgcg ttcgccacgt agccagacgt agcccatcag
    54601 cgcatacacc acgccagaaa gcccgccaaa ccacggcccg ctgaattttt gctgcacata
    54661 gccgcttaac agggcgctga taagcgtaat gacaattagc ttaccgctac cgaggcgttt
    54721 ttccaccgca ccgccgagat accaccacca gagcaggtta aagaggatat gcatcagcga
    54781 gaagtgcatt aacgcgtggg tgaagtaacg ccagaactca aatttcagtg ctggatcgaa
    54841 tggccaagcc agccataaca tcacttcctg atcgccgaga atttgcatgg caataaacac
    54901 caccacgcag gcgatcatca tcacccaggt caccggacct gcgcgttcac gcaaggcggc
    54961 aaagaaagga taacggcgat aatgcaggcc actgccggta tggcctgcct gccagctcgc
    55021 cgccagataa cgcggatctg ccgggttttc gagaaaacgc gccagctccg cccgtacgcg
    55081 ctcggcctgg gactcatccg ccagccagac atcgctttgg ttatgttgtt gaatcgtgag
    55141 gataacaccc tgcgtcgcca tgtaatcaac aaacgcctgc gccacgcggg ggttagcaaa
    55201 agaggtaatc atcaacatcg ttgctgtcgc ttattccaca caaaagggga cagtataaag
    55261 cgttacgcgc cgtacgccac ctctgcggga aactgacgtt gccaggcttc aaagccgccg
    55321 tcaatgctat agaccacatc gtagccctgt tgcagcagat actgcgccgc gcctttgctg
    55381 ctattgccgt gataacacat caccatcacc ggagtgtcaa agtcgttatt acgcataaaa
    55441 gcgcccagcg tgtcgttggt taaatggaaa gcctgcaccg catgtcccat tgcgaaactc
    55501 tgtggatcgc gaatatcgac cagcaccgcc tctttttcct gcaacttctg gtgcgcgtcg
    55561 gcaacgttaa tacattcgaa ctgatccatg cgtctctctt tctttacaaa caagtgggca
    55621 aatttaccgc acagtttacg tcgaagcggc agataaacgc cataatgtta tacatatcac
    55681 tctaaaatgt tttttcaatg ttacctaaag cgcgattctt tgctaatatg ttcgataacg
    55741 aacatttatg aactttaacg aaagtgaatg agggcagcat ggaaaccaaa gatctgattg
    55801 tgataggtgg cggcatcaat ggtgctggta tcgcggcaga cgccgctgga cgcggtttat
    55861 ccgtgctgat gctggaggcg caggatctcg cttgcgcgac ctcttccgcc agttcaaaac
    55921 tcattcacgg tggcctgcgc taccttgagc actatgaatt ccgcctggtc agcgaggcgc
    55981 tggctgaacg tgaagtgctg ctgaaaatgg ccccgcatat cgccttcccg atgcgttttc
    56041 gcctgccaca tcgtccgcat ctgcgcccgg cgtggatgat tcgcattggt ctgtttatgt
    56101 acgatcatct gggtaaacgc accagcctgc cgggatcaac tggtttgcgt tttggcgcaa
    56161 attcagtgtt aaaaccggaa attaagcgcg gattcgaata ttccgactgt tgggtagacg
    56221 atgcccgtct ggtgctcgcc aacgcccaga tggtggtgcg taaaggcggc gaagtgctta
    56281 ctcgtactcg tgctacttct gctcgtcgcg aaaacggcct gtggattgtg gaagcggaag
    56341 atatcgatac cggcaaaaaa tatagctggc aagcgcgcgg cctggttaac gccaccggcc
    56401 cgtgggtgaa acagttcttc gacgacggga tgcatctgcc ttcgccttat ggcattcgcc
    56461 tgatcaaagg cagccatatt gtggtgccgc gcgtgcatac ccaaaagcag gcctacattc
    56521 tgcaaaacga agataaacgt attgtgttcg tgatcccgtg gatggacgag ttttccatca
    56581 tcggcaccac cgatgtggaa tacaaaggcg atccgaaagc agtgaagatt gaagagagtg
    56641 aaatcaatta cctgctgaaa gtgtataacg cgcactttaa aaaacagctt agccgtgacg
    56701 atatcgtctg gacctactcc ggtgtgcgtc cgctgtgtga tgatgagtcc gactcgccgc
    56761 aggctattac ccgtgattac acccttgata ttcatgatga aaatggcaaa gcaccgctgc
    56821 tgtcggtatt cggcggtaag ctgaccacct accgaaaact ggcggaacat gcgctggaaa
    56881 aactaacgcc gtattatcag ggtattggcc cggcatggac gaaagagagc gtgctaccgg
    56941 gtggcgccat cgagggcgac cgcgacgatt atgccgctcg cttacgccgc cgttatccgt
    57001 tcctgaccga atcgctggca cgtcattacg cgcgtactta tggcagcaac agcgagctgc
    57061 tgctcggcaa tgcgggagcg gtaagcgatc ttggggaaga tttcggtcat gagttctacg
    57121 aaacggagct gaaatatctg gtcgaccacg agtgggtccg ccgcgccgac gatgccctgt
    57181 ggcgtcgcac aaaacaaggc atgtggctga atgcggatca acaatctcgt gtgagtcagt
    57241 ggctggtgga atatacgcag cagaagttgt cgctggcgtc gtaaattaac gtaaggtgat
    57301 caggtcagat ttcaatctgg cctgagactg atgacaaaca caaaattgcc tgatgcgcta
    57361 cgcttatcag gcctacgtgg tttatgcaat atgttgaatt tgcatgattt tgtaggcctg
    57421 ataaggcgtt cacgccgcat caggcatgaa caaagcgcca cgttgtcaac aatctgacct
    57481 actttttgat tcagggaatt accgcaaagc ccacgccatc atacccaacg taacaaaact
    57541 taagcacgtt ccccactcac tccacttccg tatctggctt ctatttttgc tacgccactg
    57601 gcaataatat cccaacgcaa aaccaagctg tgcacttatc ggaccgacaa agtcgatcca
    57661 ctgttgagac caaaatggcg aaaaattcag tgggagccag acaactgtca gaaagaaact
    57721 gcgcatattt tccagcaaag caatttgcgc tggcgcgaca taaaaaatga tattccacag
    57781 gagcaaccag aaataaactg gtgcaaatac cagccaacaa tgcagtctgc ttttcaattc
    57841 aggtggcgtt ctgcgcgcaa attgccaacc aacgcccacc attgcacaga agaaaagcgc
    57901 atattcagcc gataaccata gttgtaattg cagaagggaa atacgcctgt gcatgagcgg
    57961 gattgagaac agtatttctc ccagaaaact cagcgtgatt acacaggcag ccagcgcaac
    58021 caccgtcggc cacaccaaca acggtacaat aacaaagccg agaaataacg gataaatata
    58081 aagtctgatt atattcatca ttattgtcct gatgattcgg gggatgcttc atacagcggc
    58141 acatacccca catcctctac caactgttgc ccctgctgac ttataaacca gtcaacaaat
    58201 ttctgcgttt ccggcgtggg attttcacgc gtcaccatat aggcatccac aatgtatgga
    58261 taagtgccgt tgcggatatt ttcctcggta ggcgcaacgc catttatagc caaaagttta
    58321 atctgcttgc gcattttgag catattgtca ttcatacgag tgacgtaata gtggaaggtg
    58381 tacccaatgg aagatggcgt gttttgatag tcggcaactt cagtaattaa accgcccata
    58441 agatcaatca cagtggattt tttagctggc agcattgggg tgtcttgcat aaccaacccc
    58501 ttcataatcg tttggcttcc agaatcttca gggcgctgcc agacttttat atgttcgtca
    58561 ctaccaccta ctttattcca gcggctagtt ttgccgctaa aaatatcttt aacctggtgc
    58621 tcagagagag aatttaccgg gttattaata tcaacgataa agacaaaggc ttcgcgggcg
    58681 aaagcggtat atttcagttt aacacccgct tcttctgcac gttttttctg tccttccgac
    58741 ggttgtgcaa caaaaataac cgtcgcggaa cctttaatca gactatcgta ggcttcagct
    58801 gttcgggttt gctgcaaacc atacgcctgc caggccagcg aggttcgatc cttatctttt
    58861 ggctcgggta ctaatttata atacgcggag gcatacagcg ggtataaggc tgtagcgccg
    58921 tacactctcg gccatcttga ccaatcatcg ctgtaacgta atgcaggttg cccccgcaga
    58981 gacgtcagtc catggctctc ttcttccctg acgctgttat agtacccttc ccggtcgaca
    59041 catcccgcca gtaacgccgt gcctaatagt aaaacaccgg tgaatttatt catctcgctg
    59101 attcctttct ctcgagcgtt attccattgt tagatacagc ggcacatatc ccacatcttc
    59161 taccagactc tgcccctgcg gcgttaaaaa ccattcgacc agtttttgtg tttctgacgt
    59221 tgtattttct ctcgtcacca taaatgcatc gacgatgtac gcatatttgc cgttgcgaat
    59281 gttttccgcc gtcggtgtaa taccgttaat cgctagcaat cttatatttt tatcagcatt
    59341 catttgcgtc gcgtaatagc ggaaggtata gcctattgcg ttgtttgtat tacggtattc
    59401 ggcaacgact ttaatcattc cctccatcac gcttgccact tccgtttcct gcggcgagat
    59461 catgcggact tttttcatga cctgtgattg catcactgtc tggctgccag agtcttccgg
    59521 gcgctgccag gtctggatct cctgatcgtt accgccaaca gtacgccaat tggtaattgc
    59581 accactgaaa atgtcacgca cctgttgttc agtcagggaa ttaaccggat tatccgcatt
    59641 gacgatgaaa acaaatgctt cacgggcaaa tggcgtgtat agcaaagtga cgcccgattc
    59701 ctcagcgcgt tttttctgcc cgccggaggg ttgcgccaca aaaataatat cggcatcacc
    59761 tttaacaatc cggttatatg catcgggggt acgagagttc tccagatatt cccaaacgtg
    59821 aaaatcctct ggtattacac ttaatgcata aaatgcagaa gcataaattg ggtacgcagc
    59881 cgtggcgcca tcgattcgcg gccagttttg cctaaactga atttgcggtt tgccacgcag
    59941 cggcgtcagt cgattattga gtttatcggg tcgccaggcc ctgatatcaa tattttcgcg
    60001 tacaccgaca ccaggatttt gctgcttata taaatgggcc tgatacccgg cgacaatacc
    60061 aagtaagaga agtagaaaca ggagtaagtt acggcaacgt agtccggacg tgccggataa
    60121 aggatatctg gtcggccagg cgtatcccag cgaaaaactg atatgcgccc caacgggaat
    60181 aatgaccatc caccagtact caccacccac taatgagact aaaaaaacgc ccaaccaggg
    60241 aagcataagg tttgccaacc aaccagctcc catctggcga aattgataat ctgccagatg
    60301 tagcgtgatt atccagccaa ccagggttaa tagcgcgggt aagagaaaaa agcattgccg
    60361 ccagacggct atttctctgt tttctggcgc gctatcaccg gcccacacgc gaccagagaa
    60421 aaatattatt atcgccccca gcgtcagcat aaagacgttg agtagaagat aaattgttgg
    60481 cgtgataagg atattacaaa cttcaataat accggtaagc ccgacaccca gcatggcaac
    60541 aaccgctgcc aaaaattgcg ccagtatggg gatgccgaaa aaagtcatta tcagcgaggt
    60601 caaaatccat tttctatttg gcatcattct gtccattctt cctgaatgac ggaattatac
    60661 tccccgagaa ccctcgcccc ctctggacac ttttccgaaa taaaggcgaa aaaaacggga
    60721 ccttgtggcc ccgttctgtt ttttagcgaa cttacaatct caccggatcg atatgccaga
    60781 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
    60841 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcatcg actttatcct
    60901 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
    60961 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
    61021 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
    61081 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
    61141 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
    61201 cgttaagcgc aaacttcatg ttactagtgc cggaagcttc cgtccctgcc agcgaaatct
    61261 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
    61321 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
    61381 cattgatcaa atggataata tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
    61441 aaatattcac gcgcggtacc cacttcgcat ccaggtcggc cttgatgcgg ttatagcggg
    61501 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgca
    61561 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactccgcca
    61621 gacgcttttt attctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
    61681 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
    61741 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
    61801 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
    61861 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
    61921 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
    61981 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
    62041 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
    62101 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
    62161 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
    62221 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
    62281 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
    62341 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
    62401 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
    62461 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
    62521 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
    62581 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
    62641 gcgtgttgtg gcgtttgaat tcccacgggt taccatattc cagccagtag tcaggcgact
    62701 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
    62761 aaccgcgccc cggcaaccct aacgtcgcca gagaatcgag gaagcaagcc gccagacgtc
    62821 ccaggccacc gttaccgagg cccgggtcat tttcttcatc aatcagctct tcgagattta
    62881 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
    62941 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
    63001 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
    63061 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
    63121 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
    63181 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
    63241 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
    63301 caaaccgcca cagagaagga cgggaccaca gtacaaaagc acgtcgaata gcccgtaaca
    63361 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
    63421 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
    63481 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
    63541 gaatgacgtc cgcaccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
    63601 cgcccacctg gccggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
    63661 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagacccggt aaggcctcca
    63721 gcaccaggtc gaggcctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
    63781 tatcgtcaac cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt
    63841 ccaacgtatc gcgggtgtaa cgcgaggcca atagtaagtc cgtctctgga ctccagattt
    63901 tctcgtccac gccgttcagt acgccggaaa gacgcccttc acgatgacgc tgttgcaaca
    63961 aaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
    64021 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
    64081 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
    64141 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gccggacgcc
    64201 cgcgcgccgc cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat
    64261 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
    64321 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
    64381 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
    64441 acaacagcgt gatatgcccg gcgaaggtat cacgacggga tactacctgc gcatcggtca
    64501 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
    64561 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
    64621 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
    64681 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
    64741 ttcctctgcg ttttcaccaa tcaccatgcc ttccggaata acacaagcac gatcgatgac
    64801 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
    64861 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
    64921 accggagatc acacaaccgc cggaaaccag tgagttaagg gtcatcccgt ggctaccgga
    64981 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
    65041 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgaggttcg ctttccagta
    65101 agcttccagc gtacccacat cgcgccagta cggctcggca tccggatcgg attgtacgca
    65161 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
    65221 tttgccaaag tcgtggctgg agttctcatc gcgatcgtct tcttccagca gttcatacag
    65281 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
    65341 cattgacggc gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
    65401 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
    65461 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
    65521 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cgaataatat cgaggttttg
    65581 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
    65641 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
    65701 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccggagtt
    65761 gatgcagtta gacagcgcaa agtcgataat gcggaactta ccgccgaagt gtacggccgg
    65821 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
    65881 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
    65941 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
    66001 tgccagacag ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
    66061 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
    66121 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
    66181 ggttgagcat atcgatttag ccaacggaca ttgccgtcgc cttcttccca ccagcgattc
    66241 tccaccaaag cgggaatacg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
    66301 ccactgcttg cctgcgacca gtccaaccag gttaattggt tatcctgaca gtaggcattg
    66361 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtaccctgg
    66421 gagagcaaca acgttgttaa cagggcgtga atgctgtcgc gccgccgttc aaccaggtca
    66481 agggtaccgc ctaccccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
    66541 cgattttctt ctccgtttgc ttcattgtgt ttatggttga aacaaacgca gtcgcgaagc
    66601 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
    66661 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
    66721 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
    66781 gggaaatttc ccacctgata accaccagga gcgatatccc acggttcagc aattaacttc
    66841 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
    66901 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
    66961 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccgggtg actcaaattg
    67021 agcgtgttgc cacaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
    67081 ctacggttgt cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
    67141 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
    67201 tcgcgaaact catgcagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
    67261 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
    67321 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
    67381 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
    67441 gggtgcaggt acgttaatcc tttaacatgg gcttcataaa tgatggtgct gccccacggc
    67501 gtgcgcggcg gggcatcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
    67561 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
    67621 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
    67681 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
    67741 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgtgccctgg caagtcatag
    67801 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
    67861 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
    67921 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
    67981 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
    68041 cattacctgc attactgccg tgatagtgca tggaatcggt attgaggatc tcacgccatt
    68101 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
    68161 caacgatgat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
    68221 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcatcgctt
    68281 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
    68341 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
    68401 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
    68461 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
    68521 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
    68581 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
    68641 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
    68701 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
    68761 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
    68821 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
    68881 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
    68941 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaaa
    69001 tcagcgtgtt ccagtcctga tgatagcctt cgcgcggatc gctgtgttca tacaagttcg
    69061 taccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
    69121 gaatcacgtt cagaccagct gcgtgtgcag catcaatgaa ataacggaag tcgtcgcgag
    69181 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
    69241 agggatgctc gttaaggggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
    69301 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
    69361 gccaggagcc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
    69421 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
    69481 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
    69541 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
    69601 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
    69661 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
    69721 catccatagt atctgcatgc gcgcctaaag tttcatacgg gcgcaggtga gtaccttcag
    69781 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
    69841 ggttttgctg cccatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
    69901 gaatgacgcc actaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
    69961 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
    70021 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
    70081 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
    70141 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
    70201 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
    70261 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
    70321 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
    70381 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
    70441 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
    70501 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
    70561 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
    70621 ttccacggtc ggaatcgaca catctttttt caatttaata gtgaatgcct ggctgtggca
    70681 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    70741 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    70801 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    70861 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    70921 ggtcgcgagt tcatctgcta catggccata cagatggccc atctgggtta ataactcacg
    70981 catatgccgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    71041 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    71101 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    71161 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    71221 accctgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    71281 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    71341 gccagtggtt ccgccaaaag acggcgcagc ctgaccaagc tgagaagtag aaaagaagac
    71401 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    71461 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    71521 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    71581 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    71641 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    71701 tttctgcagc agttttattg atcctgatta tggatccgct cggaaaccta cctattttca
    71761 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    71821 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    71881 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaattgcca
    71941 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    72001 catttatcgt gccgctggca attccgctgg tcgccgggcc aactattctc gccacgctga
    72061 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    72121 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    72181 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc
    72241 agatgttcct cgacggcatt cgaatgtgga tgaaggggta aggttcaaac aggtgctgca
    72301 cacaaccaga cgcggcacct gttttattac ctgaactgat aatgaccttc gcccaatcgg
    72361 ataacccttc cctcttcttc tgcgcggttc agccacacac taatttgttc gatggttaaa
    72421 ccagtacaag acgccagcct ttcaatactt atcgattcct ttgcaaggat cgccagttcc
    72481 gccacaaaga gttgataaaa atcgacaggc gtatacgcgg gcattactgc gtaatttgcg
    72541 tcatcttcat gcagcgaaaa cagttctggt tgagcgttac ggagtgctct gtcatacatc
    72601 agcccaggaa cgtctggcag tctgatcgtc acattctcag cctgttcagc actccatgac
    72661 gccccatttt ctactaatcg tgaattggct gattgcatat cctgatttgg tttaacctgc
    72721 acaggcagcc actgatgttt taatgcctcc atcgccccgg ttatcgttcc accggtcatt
    72781 cccgcacgta caaccattgc gctttcagca aggcaataaa tatatttatt tcgcgccatc
    72841 gcattgccga cggtgaaacg tacctctggg taaaacggcg aaatcaacac caggttgcct
    72901 gctataagcc cttcacgcca tttcactaac gtactcgttt ttaacaagct atcagctaat
    72961 acgccaacgg cagttccccc ggcctccagt gccgacgcca ttgcacattc atcaatacct
    73021 cgcgcaccac cagagataac gcaaatcccc tgttgggcca gtttcgcggc cagttgttgg
    73081 gtatagcgca aatcgtcagt cggagcatct cgcgagccaa caatcgccat accttccgct
    73141 tgcagtaatg ctttattccc gcagccaaat aaaacgggag gtgcatccgt tcgcaatcgg
    73201 tttttcaagc gaacgggata atcagcatct ccgcgggtta agatccataa accggcgcgt
    73261 tgccacttat ctaccgccaa cgccagacta tgaccacgcg ccagcaagcc aagaatacgc
    73321 tcctgagaaa tacgcggatc gctccagcct gtaagtttct cttgcggttg cggcaccaga
    73381 agctcggcgg gactgatacg ttggtgcttc agccataatg caaacttccc ccattcacta
    73441 ttactaagag gtttatactt actcgccgcc gcgcgagaaa aatcgctggt tagcaggaga
    73501 gttgcttgtg cattggctga aagattcatc aattttttac cgaggtagaa gcaagggcaa
    73561 gaggataaac cgtcgggcaa cctgcctggc gtaacagtgc tgttagaact gtcagcgtcc
    73621 atgcagaatc cacgatatcg tcaaccagca acgccggacc aggcatcaaa ggaggggtaa
    73681 tcacaaacgc cccgtcgaga ttttgacact ggtggaaacg gttttgctgc attttctgcg
    73741 gtggattgtc cacgactttt tcaatggcat caataaaagg taagccaagt ttcgccgcca
    73801 gtcgccgggc aaaatcagga accaggtcga ggtgctttca aaatcaggaa ccaggtcgag
    73861 gtgctttaat gaaggaacgc agcataccca ggttggttca ggatgcggat tccagcgttg
    73921 gcgaaccatt tccgcacatg cctccaccag ttcatcactg aatcgccccg tttttttctc
    73981 ctgtgctacc tgctttcccc acccggaata tccccaacga gaaaggattc gccccgtaga
    74041 tccttgtaaa ctcgcaggca agttcccttt aaatccgtat tgcgtaaaag ccccagctgc
    74101 cacctgttta ttgagattta atgacaggtc agcgtgttta ataaataagt ttgccgcatg
    74161 gagtaagccg ctatcaatgt cgggcgacaa caacggatgc tgaagacaac tgctacattt
    74221 accacaatgt tcggcacctg gctcatcgag tgcgcggcgc agaaattgca ttttgcactc
    74281 cttggtcgtg atatagcttt caacgtctgc cagctcactt tttctctgat tcatcaaatg
    74341 gttaattcgt tcatggtcag gagaaaaact gacaattgtt ctgcgccata atttctcggt
    74401 atacaccaca ggcgatggat tttcagctac caataatttc agtgcttttt ctatttgccc
    74461 ataacggaga ttcgtccgtt gttcaatgcc tcgtaaggta agaccgtcat tcacgctaag
    74521 tacgttgagt atttcatgaa tttgcgcctc cgcaggaaag gcgctttcac ggaagaattt
    74581 atgaatagca cggtcttcac tgccgcaaag cagtatgcca acagcagaat cgataccgcg
    74641 accggcacgc cccacctgtt agtagtaacc aactatcgaa cctggcatct ggtaatgaat
    74701 aacaaacccc aaatccggtt tgtcaaagcc cattcccagc gccgttgtcg caaccagcac
    74761 tttaatttta tttgccagca acgcctgctc cagatattcc ctggcagtat tagaatcttc
    74821 cgcgccttca caagtcacgc cgctgtagta agcaaatgca ctgataccat ttttccttaa
    74881 ccactcggcg acaagttcgg catcgcgagt agtcagggta taaacaattc ccgatttgga
    74941 aaactgaggg ataaccgttg ctaaccatgc cagacgggat gagtgttcac ctaagactaa
    75001 ggcatcaagc gccaaacttt cgcgagccag cgttccacgc tgaatcacaa tgtcacccaa
    75061 ttgctgacgg atatcctcaa cgacacggtt attcgctgtc gcggttgtac ccagaacagg
    75121 ggtattcgca ggtagttgcg caaaatatct aatatacgtc ggtaatccgg gcgaaaatcg
    75181 tggccccaat cagagatgca gtgcgcctcg tcgaccacaa gcaagccaat atgatcggct
    75241 ataggatata aaaccgtttc gataaagtcc tggtttgcca aacgttccgg ggaaaccagc
    75301 aagcaatcga cctcaccttg cagcaactta tcgctaatgc gctgccattc ttctctgttt
    75361 gtagaattca acgtttcagc cgtaatacct aaccgttcag cggcggcaac ctggttacgc
    75421 atcagggcaa gcaaaggaga gatgataatt gtcgggccag cgccgcgatc gcggaagatt
    75481 ttactggcga tgaagtacac agcacttttc ccccagccag tgcgttggac aaccagcagt
    75541 tttcgccgtt ggttcaccac cgcgtctatg gcttcccatt gcccttccct gaaagtagcg
    75601 gtggtatttc ctaacatccg ctgtaaaagc aattcagctg cgtgcttttc catctttatc
    75661 tcctgcgatc tttaggcagt gattagaacg gattatttgc aggtaaattt gcgagagaga
    75721 tcaaaaagat gaagagagga gaaaagagaa atgaattact gcgagcaagc tcgcagtaat
    75781 ttgtcgcagt aattaactca acagctggaa cgcaatcatc ccaacgatgg caccgacagt
    75841 gccgaggatg gtttccatca tggtccaggt tttcagcgtt tcggcttcgg tcgcgccggt
    75901 aaatttaccg aacaaccaga aaccagcgtc gttaacgtgg ctgacaacaa tcgaaccacc
    75961 ggcgatacaa atcgacagcg ccgccatttg cgcaccggag tagttcagtt gttcaataac
    76021 cggcatcacc agtcccaccg ccgttaaaca ggcaacggtt gcagaaccct gaatgatgcg
    76081 cactgcagcg gccagcacga agcaggtgat ggcaatcggc aggcccatgc cggttaacgc
    76141 ttcgcccagt gccggaccta cgccagagtc aaccagcacc tgtttgaaca cgccgcccgc
    76201 accaatcacc agcagaatga tccccgccgg ttgcagcgcg tgaccgcaaa tctccatcac
    76261 tttgtctttt ggcatgccct gacgcattgc caggccataa atcgctacca gacaagcaac
    76321 cagaatcgcg gtaaacggat gaccaataaa ctcgaaccat tcgtaagcgg tagagccttc
    76381 cggcacaaaa cgcgcggcaa tggttttcag ccctaccagc accagcggca gcaggatcag
    76441 cgacaggctg aatccgaagg atggcatttt gccttcgccg agatgcggtt cgctgatgtc
    76501 gtcaggaata tgcagctcaa cgtaacggct gatgaagtta ccccacagcg gcccggcaat
    76561 aatcattccc ggaattgccg cacacaggcc aatcaggatc atccagccaa aatcggcgtt
    76621 catctgcgat gccagcagca ttggcgctgg ccctggcacc aggaacgccg ccgcggccgc
    76681 cacgcctgcg aataatggga ttaccagctt caccaggttc gtaccggtgt ggcgcgccat
    76741 tgagaaagca acgctaatca gcagaacaat cgccacttca aagaacagcg gtagcgcaca
    76801 gaccagcccc gcaaggccga tggcataatg cgcgcggctg tgaccgaagg atttaagcat
    76861 tttgacggca atctgatcga ctgcgccggt ttcatgtaag atcttgccaa acatagctcc
    76921 cagggcgaca accaccgcca ggaagccgag ggtgcctccc atcccttttt ccatcgtcgc
    76981 tgcgatttta tcgagcggca taccggaaaa aaggccagcc cccatggaca ccaccattaa
    77041 agccaggaaa gcgtgcatac gcgccttcat gactaaaaac agcagcagta aaacagaccc
    77101 tactgctgtt aaaacaagcg ttaatgtagt cactacttat ttgccttttt taataacctc
    77161 aatggtgctt gccacaacac cttccagcgg ttgatcgata tccaccacca gtacatcggt
    77221 ttcgtccgca cccggctcct gcagcgtttc aaactgcgtc accaacattt gggttttaaa
    77281 gaaatggcct ttgcgcgctt tcaggcggct ttcaatcaca tcaaaatcgc ctttcaggta
    77341 gatgaaagag agattcggat taccttcacg cagcaagtca cgatagtgtt ttttcaatgc
    77401 agaacagacg atcagcgaca ctttattagt gcgctgcata gcaaacgcgg cgtcgttcag
    77461 cgcctgcaac cacggtttgc gatcgtcgtc attcagcggt tcgccagacg ccattttttc
    77521 gatattgcgc cgtggatgga ggaaatcgcc atcaagaaac gcggcatgaa gttgatgcgc
    77581 cacttcactt gcgaccgcag atttgccgct gcccgatacg cccatcaaga cgtaaatgtg
    77641 gtgatcatgg ttagtcgtgc tcaaagtggt gcccccacaa tacaagaatt aaaaatgtta
    77701 cgggtaactg ttatcggtaa cattgtccag ccggacaata tcagaagcaa tatccatccg
    77761 tgccttaagt gtataagtgt gagctacttc aaatttgtgg gcttaaatag atccgcccgg
    77821 tgacaaggtg aaacctaaat ctaacatttt cggtgtcaca gattcgccac gaatacgcgc
    77881 cagcaggcgt tcagcgccaa tactgcccat ccgctcacgc ggcgtcagca cgctcgcaag
    77941 tcgtggctcc atcacctgac caatgtcatg accgtggaaa ccggcaatcg ccatatcgtc
    78001 aggaactttt aaccccagac gctgacattc aaacgccgcg ccgaccgcca gatcatcatt
    78061 agtacagaac acgccatcca gctgcggata ttcccgccgt gcctggcgaa tcagttcaat
    78121 accggaagag taagaagaag attgctcaac catcacgcta tatggcacca ggcctgcatc
    78181 cagcatcgcc tgttcgtatc ccttctgttt gatgatagta cgttcgtcga gacgtgcgcc
    78241 gagataggca atgtggcgat gcccgcgagc aataatggct gtggtcatct ggcgtgccgc
    78301 ttcaaagtta tcaaaaccga cggcgatatc gagacacggc gactggctgt ccatcaactc
    78361 caccaccggg atccccgcca cttcaatcat ctttaaggtg cgcggcgtgt gggtacgttc
    78421 ggtgaggatc agtccgtcga tattccatga gagcattgat tcgagacgtt cttgctccat
    78481 ttccggttta taaccgtagt gggccagcat ggtctgataa ccgtgcgcgt cggtgacgct
    78541 ttcgattccg cgtaatactt ccgcgaaaac ctggttggtg agagaaggta acaggacgcc
    78601 aatcgcccgg ctggtggcgt tagagaggat atcgggcgca cgattgggaa tatagcccag
    78661 ttcatcaaga gccgcggcaa tcttgccgcg tagagcgacg gaaacctgct ccgggttgcg
    78721 taaaaaacgg ctgaccgtca ttttggtcac gcctacacgg tcagccacat cctgaagtac
    78781 gggtcttttc tttttcatcg tcctgaaggt acaaaagaga tagattctct tagtttaaca
    78841 cggacgcatc agaaccttcc cggggaaaac agtgcttgtt tacaattatt agatatacac
    78901 taaatcaatt gcatcgcatt gtgctaacga cgctgcaaaa ccccggaagc gtacatcagc
    78961 acgcgaccgg agcgagcgag gaagcctgca aaaaggcagg cttcaaagat gccgttgagc
    79021 tttaaaccgg cggcagatcg aacagtaaca cttcgctgtc gctatccgca tggatggaga
    79081 ttgcctgctc atcccagatt gccagaccat cgctgatcga ggctttcacg ccgttaatgg
    79141 tgacattgcc tttcaccacc tggatccaga cgcggcgttc agcggcaatc tgatgcaccg
    79201 actgctcatc tttcagcaac gcccagcggt acagttccat atcctgatgc actttcaatg
    79261 aaccatcacg cgcatctggc gaaagcacca gttgtttgcc ctgcactgca tcgaagcgac
    79321 gctgttcata acgcggcgta atgccgtttt cttccggcat gatccagatc tgatacaggt
    79381 gcagacgctc ggtgctgctt gggttgtact ctgagtgacg aatacccgta cccgcactca
    79441 taatctggaa ctcgcccgcc ggaacctgct ctttattgcc catgctgtcc tggtgctcaa
    79501 cagtaccttc cagcacgtag gtcaaaattt ccatatcttt atgcgggtga gtgccgaagc
    79561 cctgccctgc ttcaatcacg tcgtcgttaa tcacgcgcag cgcggagaag cccataaagt
    79621 tcggatcgta atagttggca aaagagaaag tatgccagga gtccagccag ccatgatttg
    79681 catgaccacg ttcatttgct ttgcgtaagt agatcatttg tattcgcccc ctgaatgatt
    79741 tcgatgggct tagtgtggac ccgatctgcc tgggatgata gagggtgaaa attgacccct
    79801 ctgttcaaaa aaattgaact attcgagggg ccatcaaact tacttcgcga gagtcactgt
    79861 ggagggagag gcttgctcga atccacgttc aagtatttcc agattcgtaa gaacttcaga
    79921 ttccttgacg taatttggcg caccgtgggt gatggtttga tacaacgcat cataaacgcg
    79981 cccgtaatcg cccatctccg gcttcatctc ttctctgacc gtcacgccct cgtcattgac
    80041 atactccagc acaccgaccg aatcatccgc tgcgaatccc ggttcgcccg gcataatatt
    80101 agccttcagg ctggtttcct gctggtcgat accatattta ataaacgaac ctttcttacc
    80161 gtgaacgata aatttcggat aatcgatttt caccagatgg ctggttttga cgatggcttt
    80221 tagatcgcca taaaacagtt gcgcttcgaa agtatcgtca ggattggctt tattgcgcag
    80281 gctgcggatg tcataagcga cgtgatccgg gcgaccgaac agagaaataa tctggtccat
    80341 cgtatgcaca ccaaggccat agaacgcgcc atcctgcggc agcccaggtt tggtttctgc
    80401 caccgggcgg taatagtcaa aatggctttc cacttcgaca atctctccca gcttgccgct
    80461 ttcaatcgct tttttcgccg tcaggaagca ggaatcaaag cgacgattct gatatggcgt
    80521 gacggtcagc cctttgcttt tcgccagtgc aaacagctct ttcgcctgcg caagtgtcgg
    80581 agtgaacggt ttttcgacca gcacattttt cccggcttcc atcgcgcgct tcgcgtactc
    80641 gaagtggctg tccgcatggg tgcagacaac aaccagctta acatcgggat cgtttagcac
    80701 ttcgttgaga tcgctggtga aatggatatg agagtaaatg ggagcttgtt cttccggctt
    80761 tgcatggcgg cgaaaaatat gcgcgacatg ccagctatcc ttgcggttaa gtacatacgg
    80821 cagatggtaa cgggtggtgc ttttgccgaa gccaataaag gcgcagttga tgaccatgat
    80881 ttcgtccttt ttaaggtggt tatttacacc ttagcgcaaa gcggacgtgg ttcctactgg
    80941 agtgcgcata acgaacacaa gcacttcccg aggataaatt gagaacgaaa gatcaaaaaa
    81001 aaagccagca cccggctggc taagtaatac tggaagcaat gtgagcaatg tcgtgctttc
    81061 aggttctccg cgagggtctt cctgatcgcg agacaataat aatcattctc attcgcactt
    81121 gtccaacact ttttgcaaaa aaatgcattt gactcgcatt cgaaagtcaa tgatgttgaa
    81181 agggacattt accccaaaga ggacaaagga atgagtgaga tagtgatacg ccatgctgaa
    81241 acacgggatt acgaggccat caggcagatt cacgcccagc cggaggtgta ttacaacaca
    81301 ctacaggtgc ctcatccttc cgatcatatg tggcaggagc gactcgccga tcgtcccggc
    81361 atcaagcaac tcgtcgcctg tattgatgga gacgtcgtgg gccatctcac cattgacgtg
    81421 caacagcgcc cacgccgcag tcatgttgcc gattttggta tctgtgtcga cgcccgctgg
    81481 aagaatcgcg gcgtcgccag cgccctgatg cgagagatga ttgaaatgtg cgacaactgg
    81541 ttgcgggtag atcgtattga gctaacggtg ttcgtcgata acgcgccagc aattaaggtc
    81601 tataaaaaat tcggctttga gattgaaggg actggcaaga agtacgcatt acgtaatggt
    81661 gaatatgttg atgcgtattt tatggcgcgg gtgaagtaaa gaatattctc aaattgcctg
    81721 ttaccaatgt gaataattag ttttgttcat ctaatttgag tttttcctta tattaccacc
    81781 atcattttga aacaatttgt aacatcaaga gacctttgaa tcattactcg ttaatatttc
    81841 ttattaaaga gaacatattt tcgcaagcaa tagttattac tgtttgttta tggatagaga
    81901 gtctacttaa tgggtacagc agctataagt catttacgat atgatcttaa caaatatgcc
    81961 ctttctttac gcaaaaccgc aacacttgct tcaacatttt ttattgagtc cccattagtt
    82021 agattcgagt atctgcaaga aatagaaaat actattaatg atattactca cagattcaat
    82081 tcttcatatg acatcaatga aaaagcacga cttatcaatg aattaaaaat ggagtcagaa
    82141 acagcaagaa aagaatatca acttttcaga caaggaaatt atgataaata tatcactact
    82201 gatattttcg aagaacatgg tttgataaaa tatgtgaact taagtttcga tattgttgca
    82261 tctgtaggac aggtcgtagg cggggttggc gcgttgaaat ttggtaaggt agtgcattca
    82321 aaccgaataa aaggtatagg cgtcacatta gttgctcatg gggcgaataa tttttatgaa
    82381 agcttatcac ctttattttt taacgagtat gattcaggtc caatacgtga actctacaga
    82441 gtaatagcaa aaaaaatggg aggtgatatt aattctgctg attatgcata cagcatcgtg
    82501 gacttttcga ttactgcata tggtggatat tcaggcatga aaatcgtacc taagtataat
    82561 agattgattc gtccttcatt aggaaataga cctggtacag gacgattatt tcattacaca
    82621 tcagttgatt ttaaaaataa attctcatta aaacctaccc ctttaaaaat c
//
LOCUS       DABSYT020000012        80524 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.012, whole genome shotgun sequence.
ACCESSION   DABSYT020000012 DABSYT020000000
VERSION     DABSYT020000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 80524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.012"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            147..1844
                     /gene="ypdA"
                     /locus_tag="ID725_RS05500"
                     /old_locus_tag="pgaptmp_001100"
     CDS             147..1844
                     /gene="ypdA"
                     /locus_tag="ID725_RS05500"
                     /old_locus_tag="pgaptmp_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGV7849749.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1859..2593
                     /gene="ypdB"
                     /locus_tag="ID725_RS05505"
                     /old_locus_tag="pgaptmp_001101"
     CDS             1859..2593
                     /gene="ypdB"
                     /locus_tag="ID725_RS05505"
                     /old_locus_tag="pgaptmp_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGV7849750.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2606..3463
                     /gene="ypdC"
                     /locus_tag="ID725_RS05510"
                     /old_locus_tag="pgaptmp_001102"
     CDS             2606..3463
                     /gene="ypdC"
                     /locus_tag="ID725_RS05510"
                     /old_locus_tag="pgaptmp_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7849751.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3466..5961)
                     /gene="ptsP"
                     /locus_tag="ID725_RS05515"
                     /old_locus_tag="pgaptmp_001103"
     CDS             complement(3466..5961)
                     /gene="ptsP"
                     /locus_tag="ID725_RS05515"
                     /old_locus_tag="pgaptmp_001103"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGV7849752.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5986..7023)
                     /gene="ypdE"
                     /locus_tag="ID725_RS05520"
                     /old_locus_tag="pgaptmp_001104"
     CDS             complement(5986..7023)
                     /gene="ypdE"
                     /locus_tag="ID725_RS05520"
                     /old_locus_tag="pgaptmp_001104"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGV7849753.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7023..8108)
                     /gene="ypdF"
                     /locus_tag="ID725_RS05525"
                     /old_locus_tag="pgaptmp_001105"
     CDS             complement(7023..8108)
                     /gene="ypdF"
                     /locus_tag="ID725_RS05525"
                     /old_locus_tag="pgaptmp_001105"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGV7849754.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8123..9370)
                     /gene="fryC"
                     /locus_tag="ID725_RS05530"
                     /old_locus_tag="pgaptmp_001106"
     CDS             complement(8123..9370)
                     /gene="fryC"
                     /locus_tag="ID725_RS05530"
                     /old_locus_tag="pgaptmp_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGV7849755.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9392..9718)
                     /gene="fryB"
                     /locus_tag="ID725_RS05535"
                     /old_locus_tag="pgaptmp_001107"
     CDS             complement(9392..9718)
                     /gene="fryB"
                     /locus_tag="ID725_RS05535"
                     /old_locus_tag="pgaptmp_001107"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGV7849756.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9937..10902)
                     /gene="glk"
                     /locus_tag="ID725_RS05540"
                     /old_locus_tag="pgaptmp_001108"
     CDS             complement(9937..10902)
                     /gene="glk"
                     /locus_tag="ID725_RS05540"
                     /old_locus_tag="pgaptmp_001108"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGV7849757.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11106..12362
                     /gene="yfeO"
                     /locus_tag="ID725_RS05545"
                     /old_locus_tag="pgaptmp_001109"
     CDS             11106..12362
                     /gene="yfeO"
                     /locus_tag="ID725_RS05545"
                     /old_locus_tag="pgaptmp_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGV7849758.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12477..12803
                     /gene="ypeC"
                     /locus_tag="ID725_RS05550"
                     /old_locus_tag="pgaptmp_001110"
     CDS             12477..12803
                     /gene="ypeC"
                     /locus_tag="ID725_RS05550"
                     /old_locus_tag="pgaptmp_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGV7849759.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12943..14181)
                     /gene="mntH"
                     /locus_tag="ID725_RS05555"
                     /old_locus_tag="pgaptmp_001111"
     CDS             complement(12943..14181)
                     /gene="mntH"
                     /locus_tag="ID725_RS05555"
                     /old_locus_tag="pgaptmp_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGV7849760.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14517..15719
                     /gene="nupC"
                     /locus_tag="ID725_RS05560"
                     /old_locus_tag="pgaptmp_001112"
     CDS             14517..15719
                     /gene="nupC"
                     /locus_tag="ID725_RS05560"
                     /old_locus_tag="pgaptmp_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGV7849761.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15769..17958)
                     /gene="pdeA"
                     /locus_tag="ID725_RS05565"
                     /old_locus_tag="pgaptmp_001113"
     CDS             complement(15769..17958)
                     /gene="pdeA"
                     /locus_tag="ID725_RS05565"
                     /old_locus_tag="pgaptmp_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGV7849762.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18166..18241)
                     /locus_tag="ID725_RS05570"
                     /old_locus_tag="pgaptmp_001114"
     tRNA            complement(18166..18241)
                     /locus_tag="ID725_RS05570"
                     /old_locus_tag="pgaptmp_001114"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18206..18208),aa:Ala,seq:ggc)
     gene            complement(18281..18356)
                     /locus_tag="ID725_RS05575"
                     /old_locus_tag="pgaptmp_001115"
     tRNA            complement(18281..18356)
                     /locus_tag="ID725_RS05575"
                     /old_locus_tag="pgaptmp_001115"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18321..18323),aa:Ala,seq:ggc)
     gene            18577..18936
                     /gene="yfeC"
                     /locus_tag="ID725_RS05580"
                     /old_locus_tag="pgaptmp_001116"
     CDS             18577..18936
                     /gene="yfeC"
                     /locus_tag="ID725_RS05580"
                     /old_locus_tag="pgaptmp_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGV7849763.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18938..19330
                     /gene="yfeD"
                     /locus_tag="ID725_RS05585"
                     /old_locus_tag="pgaptmp_001117"
     CDS             18938..19330
                     /gene="yfeD"
                     /locus_tag="ID725_RS05585"
                     /old_locus_tag="pgaptmp_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGV7849764.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19370..20578)
                     /locus_tag="ID725_RS05590"
                     /old_locus_tag="pgaptmp_001118"
     CDS             complement(19370..20578)
                     /locus_tag="ID725_RS05590"
                     /old_locus_tag="pgaptmp_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGV7849765.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            20586..21011
                     /gene="tnpA"
                     /locus_tag="ID725_RS05595"
                     /old_locus_tag="pgaptmp_001119"
                     /pseudo
     CDS             20586..21011
                     /gene="tnpA"
                     /locus_tag="ID725_RS05595"
                     /old_locus_tag="pgaptmp_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(21129..22544)
                     /gene="gltX"
                     /locus_tag="ID725_RS05600"
                     /old_locus_tag="pgaptmp_001120"
     CDS             complement(21129..22544)
                     /gene="gltX"
                     /locus_tag="ID725_RS05600"
                     /old_locus_tag="pgaptmp_001120"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGV7849766.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            22803..22878
                     /locus_tag="ID725_RS05605"
                     /old_locus_tag="pgaptmp_001121"
     tRNA            22803..22878
                     /locus_tag="ID725_RS05605"
                     /old_locus_tag="pgaptmp_001121"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22836..22838,aa:Val,seq:tac)
     gene            22923..22998
                     /locus_tag="ID725_RS05610"
                     /old_locus_tag="pgaptmp_001122"
     tRNA            22923..22998
                     /locus_tag="ID725_RS05610"
                     /old_locus_tag="pgaptmp_001122"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22956..22958,aa:Val,seq:tac)
     gene            23045..23120
                     /locus_tag="ID725_RS05615"
                     /old_locus_tag="pgaptmp_001123"
     tRNA            23045..23120
                     /locus_tag="ID725_RS05615"
                     /old_locus_tag="pgaptmp_001123"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23078..23080,aa:Val,seq:tac)
     gene            23125..23200
                     /locus_tag="ID725_RS05620"
                     /old_locus_tag="pgaptmp_001124"
     tRNA            23125..23200
                     /locus_tag="ID725_RS05620"
                     /old_locus_tag="pgaptmp_001124"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23158..23160,aa:Lys,seq:ttt)
     gene            23320..23661
                     /locus_tag="ID725_RS05625"
                     /old_locus_tag="pgaptmp_001125"
     CDS             23320..23661
                     /locus_tag="ID725_RS05625"
                     /old_locus_tag="pgaptmp_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HGV7849767.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(23652..24578)
                     /gene="yfeR"
                     /locus_tag="ID725_RS05630"
                     /old_locus_tag="pgaptmp_001126"
     CDS             complement(23652..24578)
                     /gene="yfeR"
                     /locus_tag="ID725_RS05630"
                     /old_locus_tag="pgaptmp_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7849768.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            24668..25666
                     /gene="yfeH"
                     /locus_tag="ID725_RS05635"
                     /old_locus_tag="pgaptmp_001127"
     CDS             24668..25666
                     /gene="yfeH"
                     /locus_tag="ID725_RS05635"
                     /old_locus_tag="pgaptmp_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGV7849769.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(25663..25881)
                     /gene="ypeB"
                     /locus_tag="ID725_RS05640"
                     /old_locus_tag="pgaptmp_001128"
     CDS             complement(25663..25881)
                     /gene="ypeB"
                     /locus_tag="ID725_RS05640"
                     /old_locus_tag="pgaptmp_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGV7849770.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(25883..27898)
                     /gene="ligA"
                     /locus_tag="ID725_RS05645"
                     /old_locus_tag="pgaptmp_001129"
     CDS             complement(25883..27898)
                     /gene="ligA"
                     /locus_tag="ID725_RS05645"
                     /old_locus_tag="pgaptmp_001129"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGV7849771.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(27969..28967)
                     /gene="zipA"
                     /locus_tag="ID725_RS05650"
                     /old_locus_tag="pgaptmp_001130"
     CDS             complement(27969..28967)
                     /gene="zipA"
                     /locus_tag="ID725_RS05650"
                     /old_locus_tag="pgaptmp_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGV7849772.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            29197..29958
                     /gene="cysZ"
                     /locus_tag="ID725_RS05655"
                     /old_locus_tag="pgaptmp_001131"
     CDS             29197..29958
                     /gene="cysZ"
                     /locus_tag="ID725_RS05655"
                     /old_locus_tag="pgaptmp_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGV7849773.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            30143..31114
                     /gene="cysK"
                     /locus_tag="ID725_RS05660"
                     /old_locus_tag="pgaptmp_001132"
     CDS             30143..31114
                     /gene="cysK"
                     /locus_tag="ID725_RS05660"
                     /old_locus_tag="pgaptmp_001132"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGV7849774.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            31498..31755
                     /gene="ptsH"
                     /locus_tag="ID725_RS05665"
                     /old_locus_tag="pgaptmp_001133"
     CDS             31498..31755
                     /gene="ptsH"
                     /locus_tag="ID725_RS05665"
                     /old_locus_tag="pgaptmp_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGV7849775.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            31800..33527
                     /gene="ptsI"
                     /locus_tag="ID725_RS05670"
                     /old_locus_tag="pgaptmp_001134"
     CDS             31800..33527
                     /gene="ptsI"
                     /locus_tag="ID725_RS05670"
                     /old_locus_tag="pgaptmp_001134"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGV7849776.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            33568..34077
                     /gene="crr"
                     /locus_tag="ID725_RS05675"
                     /old_locus_tag="pgaptmp_001135"
     CDS             33568..34077
                     /gene="crr"
                     /locus_tag="ID725_RS05675"
                     /old_locus_tag="pgaptmp_001135"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGV7849777.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(34120..34971)
                     /gene="pdxK"
                     /locus_tag="ID725_RS05680"
                     /old_locus_tag="pgaptmp_001136"
                     /gene_synonym="thiJ"
     CDS             complement(34120..34971)
                     /gene="pdxK"
                     /locus_tag="ID725_RS05680"
                     /old_locus_tag="pgaptmp_001136"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGV7849778.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            35076..35443
                     /gene="yfeK"
                     /locus_tag="ID725_RS05685"
                     /old_locus_tag="pgaptmp_001137"
                     /pseudo
     CDS             35076..35443
                     /gene="yfeK"
                     /locus_tag="ID725_RS05685"
                     /old_locus_tag="pgaptmp_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(35446..36357)
                     /gene="cysM"
                     /locus_tag="ID725_RS05690"
                     /old_locus_tag="pgaptmp_001138"
     CDS             complement(35446..36357)
                     /gene="cysM"
                     /locus_tag="ID725_RS05690"
                     /old_locus_tag="pgaptmp_001138"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGV7849779.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(36491..37588)
                     /gene="cysA"
                     /locus_tag="ID725_RS05695"
                     /old_locus_tag="pgaptmp_001139"
     CDS             complement(36491..37588)
                     /gene="cysA"
                     /locus_tag="ID725_RS05695"
                     /old_locus_tag="pgaptmp_001139"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGV7849780.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(37578..38453)
                     /gene="cysW"
                     /locus_tag="ID725_RS05700"
                     /old_locus_tag="pgaptmp_001140"
     CDS             complement(37578..38453)
                     /gene="cysW"
                     /locus_tag="ID725_RS05700"
                     /old_locus_tag="pgaptmp_001140"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGV7849781.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(38453..39286)
                     /gene="cysT"
                     /locus_tag="ID725_RS05705"
                     /old_locus_tag="pgaptmp_001141"
     CDS             complement(38453..39286)
                     /gene="cysT"
                     /locus_tag="ID725_RS05705"
                     /old_locus_tag="pgaptmp_001141"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGV7849782.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(39286..40302)
                     /gene="cysP"
                     /locus_tag="ID725_RS05710"
                     /old_locus_tag="pgaptmp_001142"
     CDS             complement(39286..40302)
                     /gene="cysP"
                     /locus_tag="ID725_RS05710"
                     /old_locus_tag="pgaptmp_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGV7849783.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(40460..41251)
                     /gene="ucpA"
                     /locus_tag="ID725_RS05715"
                     /old_locus_tag="pgaptmp_001143"
     CDS             complement(40460..41251)
                     /gene="ucpA"
                     /locus_tag="ID725_RS05715"
                     /old_locus_tag="pgaptmp_001143"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGV7849784.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(41380..42237)
                     /gene="murR"
                     /locus_tag="ID725_RS05720"
                     /old_locus_tag="pgaptmp_001144"
     CDS             complement(41380..42237)
                     /gene="murR"
                     /locus_tag="ID725_RS05720"
                     /old_locus_tag="pgaptmp_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGV7849785.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            42401..43297
                     /gene="murQ"
                     /locus_tag="ID725_RS05725"
                     /old_locus_tag="pgaptmp_001145"
     CDS             42401..43297
                     /gene="murQ"
                     /locus_tag="ID725_RS05725"
                     /old_locus_tag="pgaptmp_001145"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGV7849786.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            43301..44725
                     /gene="murP"
                     /locus_tag="ID725_RS05730"
                     /old_locus_tag="pgaptmp_001146"
     CDS             43301..44725
                     /gene="murP"
                     /locus_tag="ID725_RS05730"
                     /old_locus_tag="pgaptmp_001146"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGV7849787.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            44730..46034
                     /gene="pbp4b"
                     /locus_tag="ID725_RS05735"
                     /old_locus_tag="pgaptmp_001147"
     CDS             44730..46034
                     /gene="pbp4b"
                     /locus_tag="ID725_RS05735"
                     /old_locus_tag="pgaptmp_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGV7849788.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(46092..46991)
                     /gene="yfeX"
                     /locus_tag="ID725_RS05740"
                     /old_locus_tag="pgaptmp_001148"
     CDS             complement(46092..46991)
                     /gene="yfeX"
                     /locus_tag="ID725_RS05740"
                     /old_locus_tag="pgaptmp_001148"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGV7849789.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(47087..47662)
                     /gene="yfeY"
                     /locus_tag="ID725_RS05745"
                     /old_locus_tag="pgaptmp_001149"
     CDS             complement(47087..47662)
                     /gene="yfeY"
                     /locus_tag="ID725_RS05745"
                     /old_locus_tag="pgaptmp_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGV7849790.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(47723..48172)
                     /gene="yfeZ"
                     /locus_tag="ID725_RS05750"
                     /old_locus_tag="pgaptmp_001150"
     CDS             complement(47723..48172)
                     /gene="yfeZ"
                     /locus_tag="ID725_RS05750"
                     /old_locus_tag="pgaptmp_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGV7849791.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(48159..48584)
                     /gene="ypeA"
                     /locus_tag="ID725_RS05755"
                     /old_locus_tag="pgaptmp_001151"
     CDS             complement(48159..48584)
                     /gene="ypeA"
                     /locus_tag="ID725_RS05755"
                     /old_locus_tag="pgaptmp_001151"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGV7849792.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            48798..49667
                     /gene="amiA"
                     /locus_tag="ID725_RS05760"
                     /old_locus_tag="pgaptmp_001152"
     CDS             48798..49667
                     /gene="amiA"
                     /locus_tag="ID725_RS05760"
                     /old_locus_tag="pgaptmp_001152"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGV7849793.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            49671..50570
                     /gene="hemF"
                     /locus_tag="ID725_RS05765"
                     /old_locus_tag="pgaptmp_001153"
     CDS             49671..50570
                     /gene="hemF"
                     /locus_tag="ID725_RS05765"
                     /old_locus_tag="pgaptmp_001153"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGV7849794.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(50576..51628)
                     /gene="eutR"
                     /locus_tag="ID725_RS05770"
                     /old_locus_tag="pgaptmp_001154"
     CDS             complement(50576..51628)
                     /gene="eutR"
                     /locus_tag="ID725_RS05770"
                     /old_locus_tag="pgaptmp_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGV7849795.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(51674..52174)
                     /gene="eutK"
                     /locus_tag="ID725_RS05775"
                     /old_locus_tag="pgaptmp_001155"
     CDS             complement(51674..52174)
                     /gene="eutK"
                     /locus_tag="ID725_RS05775"
                     /old_locus_tag="pgaptmp_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGV7849796.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(52187..52846)
                     /gene="eutL"
                     /locus_tag="ID725_RS05780"
                     /old_locus_tag="pgaptmp_001156"
     CDS             complement(52187..52846)
                     /gene="eutL"
                     /locus_tag="ID725_RS05780"
                     /old_locus_tag="pgaptmp_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGV7849797.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(52856..53743)
                     /gene="eutC"
                     /locus_tag="ID725_RS05785"
                     /old_locus_tag="pgaptmp_001157"
     CDS             complement(52856..53743)
                     /gene="eutC"
                     /locus_tag="ID725_RS05785"
                     /old_locus_tag="pgaptmp_001157"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGV7849798.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(53764..55125)
                     /gene="eutB"
                     /locus_tag="ID725_RS05790"
                     /old_locus_tag="pgaptmp_001158"
     CDS             complement(53764..55125)
                     /gene="eutB"
                     /locus_tag="ID725_RS05790"
                     /old_locus_tag="pgaptmp_001158"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGV7849799.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELCSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     CIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(55137..56540)
                     /gene="eutA"
                     /locus_tag="ID725_RS05795"
                     /old_locus_tag="pgaptmp_001159"
     CDS             complement(55137..56540)
                     /gene="eutA"
                     /locus_tag="ID725_RS05795"
                     /old_locus_tag="pgaptmp_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGV7849800.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(56537..57763)
                     /gene="eutH"
                     /locus_tag="ID725_RS05800"
                     /old_locus_tag="pgaptmp_001160"
     CDS             complement(56537..57763)
                     /gene="eutH"
                     /locus_tag="ID725_RS05800"
                     /old_locus_tag="pgaptmp_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGV7849801.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(57863..59050)
                     /gene="eutG"
                     /locus_tag="ID725_RS05805"
                     /old_locus_tag="pgaptmp_001161"
     CDS             complement(57863..59050)
                     /gene="eutG"
                     /locus_tag="ID725_RS05805"
                     /old_locus_tag="pgaptmp_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGV7849802.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(59040..59876)
                     /gene="eutJ"
                     /locus_tag="ID725_RS05810"
                     /old_locus_tag="pgaptmp_001162"
     CDS             complement(59040..59876)
                     /gene="eutJ"
                     /locus_tag="ID725_RS05810"
                     /old_locus_tag="pgaptmp_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGV7849803.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(59887..61290)
                     /gene="eutE"
                     /locus_tag="ID725_RS05815"
                     /old_locus_tag="pgaptmp_001163"
     CDS             complement(59887..61290)
                     /gene="eutE"
                     /locus_tag="ID725_RS05815"
                     /old_locus_tag="pgaptmp_001163"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGV7849804.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAVDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(61302..61589)
                     /gene="eutN"
                     /locus_tag="ID725_RS05820"
                     /old_locus_tag="pgaptmp_001164"
     CDS             complement(61302..61589)
                     /gene="eutN"
                     /locus_tag="ID725_RS05820"
                     /old_locus_tag="pgaptmp_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGV7849805.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(61696..61989)
                     /gene="eutM"
                     /locus_tag="ID725_RS05825"
                     /old_locus_tag="pgaptmp_001165"
     CDS             complement(61696..61989)
                     /gene="eutM"
                     /locus_tag="ID725_RS05825"
                     /old_locus_tag="pgaptmp_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGV7849806.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(62028..63044)
                     /gene="pta"
                     /locus_tag="ID725_RS05830"
                     /old_locus_tag="pgaptmp_001166"
     CDS             complement(62028..63044)
                     /gene="pta"
                     /locus_tag="ID725_RS05830"
                     /old_locus_tag="pgaptmp_001166"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGV7849807.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(63041..63772)
                     /gene="eutT"
                     /locus_tag="ID725_RS05835"
                     /old_locus_tag="pgaptmp_001167"
     CDS             complement(63041..63772)
                     /gene="eutT"
                     /locus_tag="ID725_RS05835"
                     /old_locus_tag="pgaptmp_001167"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGV7849808.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(63769..64470)
                     /gene="eutQ"
                     /locus_tag="ID725_RS05840"
                     /old_locus_tag="pgaptmp_001168"
     CDS             complement(63769..64470)
                     /gene="eutQ"
                     /locus_tag="ID725_RS05840"
                     /old_locus_tag="pgaptmp_001168"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGV7849809.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(64445..64924)
                     /gene="eutP"
                     /locus_tag="ID725_RS05845"
                     /old_locus_tag="pgaptmp_001169"
     CDS             complement(64445..64924)
                     /gene="eutP"
                     /locus_tag="ID725_RS05845"
                     /old_locus_tag="pgaptmp_001169"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGV7849810.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(64937..65272)
                     /gene="eutS"
                     /locus_tag="ID725_RS05850"
                     /old_locus_tag="pgaptmp_001170"
     CDS             complement(64937..65272)
                     /gene="eutS"
                     /locus_tag="ID725_RS05850"
                     /old_locus_tag="pgaptmp_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGV7849811.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(65565..67844)
                     /gene="maeB"
                     /locus_tag="ID725_RS05855"
                     /old_locus_tag="pgaptmp_001171"
     CDS             complement(65565..67844)
                     /gene="maeB"
                     /locus_tag="ID725_RS05855"
                     /old_locus_tag="pgaptmp_001171"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGV7849812.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            68133..69083
                     /gene="tal"
                     /locus_tag="ID725_RS05860"
                     /old_locus_tag="pgaptmp_001172"
     CDS             68133..69083
                     /gene="tal"
                     /locus_tag="ID725_RS05860"
                     /old_locus_tag="pgaptmp_001172"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGV7849813.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            69103..71106
                     /gene="tkt"
                     /locus_tag="ID725_RS05865"
                     /old_locus_tag="pgaptmp_001173"
     CDS             69103..71106
                     /gene="tkt"
                     /locus_tag="ID725_RS05865"
                     /old_locus_tag="pgaptmp_001173"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGV7849814.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALVRQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(71201..72244)
                     /gene="ypfG"
                     /locus_tag="ID725_RS05870"
                     /old_locus_tag="pgaptmp_001174"
     CDS             complement(71201..72244)
                     /gene="ypfG"
                     /locus_tag="ID725_RS05870"
                     /old_locus_tag="pgaptmp_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGV7849815.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(72370..72945)
                     /gene="nudK"
                     /locus_tag="ID725_RS05875"
                     /old_locus_tag="pgaptmp_001175"
     CDS             complement(72370..72945)
                     /gene="nudK"
                     /locus_tag="ID725_RS05875"
                     /old_locus_tag="pgaptmp_001175"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGV7849816.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            complement(73013..74992)
                     /gene="aegA"
                     /locus_tag="ID725_RS05880"
                     /old_locus_tag="pgaptmp_001176"
     CDS             complement(73013..74992)
                     /gene="aegA"
                     /locus_tag="ID725_RS05880"
                     /old_locus_tag="pgaptmp_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGV7849817.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            75198..76898
                     /gene="narQ"
                     /locus_tag="ID725_RS05885"
                     /old_locus_tag="pgaptmp_001177"
     CDS             75198..76898
                     /gene="narQ"
                     /locus_tag="ID725_RS05885"
                     /old_locus_tag="pgaptmp_001177"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGV7849818.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            77062..80175
                     /gene="acrD"
                     /locus_tag="ID725_RS05890"
                     /old_locus_tag="pgaptmp_001178"
     CDS             77062..80175
                     /gene="acrD"
                     /locus_tag="ID725_RS05890"
                     /old_locus_tag="pgaptmp_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGV7849819.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(80274..80333)
                     /gene="ypfM"
                     /locus_tag="ID725_RS05895"
                     /old_locus_tag="pgaptmp_001179"
     CDS             complement(80274..80333)
                     /gene="ypfM"
                     /locus_tag="ID725_RS05895"
                     /old_locus_tag="pgaptmp_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGV7849820.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        18952 a        22029 c        20499 g        19044 t
ORIGIN      
        1 agtgcattat caagagatgc attgctaata caccatttat ctatcaatcc ccttttaaaa
       61 ttgtggtttt tcctcaaaaa cggcctgctg gtcatcagcc ttcaggttac ctatcataga
      121 ggtttaatcc ttattcagag tcacccgtgc acgaaatatt caacatgctg ctggcggtct
      181 tcgatcgggc agcgttaatg cttatctgcc tgttctttct catccgtatc cgcctgtttc
      241 gcgaactgtt gcacaagtcg gcacactcac cgaaagagtt gctcgccgtt accgccattt
      301 tctcgctgtt cgccctcttc agcacctggt ccggcgttcc cgtagaaggc tcgctggtga
      361 acgtacgtat tatcgcggtg atgtccggcg ggattctgtt tggcccgtgg gtgggcatca
      421 ttaccggcgt gattgcgggt attcatcgtt atttaattga tatcggtggc gtgacggcga
      481 tcccctgctt tatcaccagc attctggctg gttgtatatc gggctggatc aacctgaaaa
      541 tccccaaagc acagcgctgg cgcgtcggta ttctcggcgg catgttgtgt gaaacgttga
      601 ccatgattct ggtgattgtc tgggcaccaa ctaccgcgtt ggggatcgat atcgtctcta
      661 aaatcgggat tccaatgatc ctcggcagcg tctgtatcgg ctttattgtg cttctagtgc
      721 aaagcgttga gggcgaaaaa gaggccagcg ccgcgcggca ggccaagttg gcgctggata
      781 tcgccaacaa aacgctacct ctgtttcgcc atgtcaatag cgagtcatta cgcaaagttt
      841 gtgaaattat tcgcgatgac attcacgccg atgcggtggc gattaccaat accgatcatg
      901 tgctggccta tgttggtgtg ggtgaacata actatcagaa tggcgatgac ttcattagcc
      961 cgactacccg tcaggcgatg aattacggaa aaatcatcat taaaaacaat gatgaagccc
     1021 accgcacacc agagattcat tccatgctgg tgatcccatt gtgggagaaa ggcgttgtga
     1081 ccggaacgct gaaaatttac tactgccacg cgcatcagat aacctcgtca ttacaggaaa
     1141 tggcggttgg tctgtcgcaa atcatctcca cgcaactgga ggtttcacgc gccgagcagc
     1201 tacgtgaaat ggcaaataag gcagagcttc gcgccctgca aagcaaaatt aatccccatt
     1261 tcctgtttaa cgctttgaac gctatttcat cgtcgatccg tctgaatccg gataccgctc
     1321 gccagctgat ctttaatctg tcgcgttatc tgcgctataa cattgaatta aaagacgatg
     1381 agcaaatcga tatcaaaaaa gagctgtatc aaattaaaga ctatattgcc attgagcagg
     1441 cccgctttgg cgacaagctg acggttatct atgatattga tgaagaggtg aattgctgca
     1501 ttcccagcct gctgatccag ccgttggtgg agaatgccat tgtccacggt atccagcctt
     1561 gcaaaggtaa aggcgttgtc accatcagcg ttgcagagtg cggcaatcgg gtacgcattg
     1621 cggtgcggga taccgggcac ggcatcgatc caaaggtgat tgagcgggtc gaagcgaatg
     1681 aaatgccggg caataaaatt ggcctgctga atgtccatca tcgcgtgaag ttattgtatg
     1741 gcgaggggct gcatatccgc cgcctggaac caggaacgga aattgcgttt tacattccta
     1801 accaacgcac cccagtcgcc tcacaggcta cgttattgct ttgagccgga gtgatattgt
     1861 gaaagtcatc attgttgaag acgaattcct ggcacaacag gaactgagct ggctaattaa
     1921 agagcacagc cagatggaga ttgtcggcac ctttgacgac ggtctggacg tgttgaagtt
     1981 tttgcagcat aaccgcgtcg acgccatttt tctggatatc aatattccgt cgctggatgg
     2041 tgtgttgctg gcacaaaaca tcagccagtt cgcccataaa ccgtttattg tgttcatcac
     2101 cgcgtggaaa gaacatgcgg tagaagcgtt tgaactggag gcgtttgact acattctaaa
     2161 accgtatcag gagtcacgca ttaccgggat gctgcaaaaa ctggaagcag cctggcaaca
     2221 acagcagacc agcagcacgc ctgccgcgac ggtaacgcgt gagaatgaca ccattaatct
     2281 ggtgaaagat gagcgaataa tcgtcacgcc aattaacgat atctattacg ccgaagcgca
     2341 cgagaaaatg acctttgttt atacgcggcg tgaatcctac gtaatgccga tgaacattac
     2401 cgaattttgc agcaaactgc cgccgtcgca ttttttccgc tgccatcgct cattttgtgt
     2461 caatctgaac aaaatccgcg aaatcgaacc gtggtttaat aacacctata ttctgcggct
     2521 gaaagatctg gattttgaag tgccggtcag ccgcagcaaa gttaaagaat tccgccagtt
     2581 aatgcatctt taatcgggaa ctttcatgaa agcaccaggc ttgcccgccg atcaacaatt
     2641 tttcgccgat ctgttcagcg gcctggtgct taacccgcaa ctactggggc gcgtctggtt
     2701 tgccagccag cctgcctcgt tgccggtggg cagtttatgt attgattttc cccgtctgga
     2761 tatcgtgctg cgcggcgaat acggcaatct gctggaagca aagcagcaac gtttggtgga
     2821 aggagaaatg ctgtttattc cggcgcgcgc ggctaattta ccggtcaaca acaaaccggt
     2881 gatgctgtta agcctggtgt tcgctccgac ctggcttggg ttatcgtttt acgatagccg
     2941 caccacgtca ttgttgcatc ctgctcgcca gatccagctt cccagcctgc aacgcggtga
     3001 aggtgaagcg atgcttaccg ccctcaccca tcttagccgt tcgccgctgg agcaaaatat
     3061 cattcagccg ctggtgttaa gtttgctgca tctttgccgt aacgtggtga atatgccgcc
     3121 gggcaattcg cagccgcgcg gcgattttct ctatcacagc atttgtaact gggttcagga
     3181 taattatgcc cagccgctca cccgcgagag cgtggcgcag ttttttaata tcacgcccaa
     3241 tcatctgtca aaactgtttg ctcagcatgg aacgatgcgt tttatcgagt atgtgcgttg
     3301 ggtgcgaatg gcgaaggcga ggatgatttt gcagaaatat catttgtcaa ttcatgaagt
     3361 ggcacagcgt tgcggttttc cggatagcga ctatttttgt cgcgttttcc ggcgtcagtt
     3421 tggtctgacg ccgggagagt acagcgcccg ttttcagggc taacgtcaga aggttaattc
     3481 tgtttccagc agcgtcagga tactttgtgc atcttgcgcg gcaaacaacg actggcggaa
     3541 gtttttattc accagtttac gcgccagctg cgagaagact ttcacatgat tcatcccttc
     3601 gttagcaccc agcgtcagca tgatcaccag ttcgacttcg cccatttctg actgccagtc
     3661 aaccggtttc gccagccggg caatgctgat actggaatga cggatccact gagatttggt
     3721 gtgcgggatc gccacgccaa aaccaacgcc agtggtgaca atctcttccc gctgccagac
     3781 atcctcttcc agctcgaacg gatgttcagt gcgcccgtta acgccgaggt taccgcacag
     3841 gaactggatc gcctgctctt tattgctaaa ggcctgatca acgaagatat tctccagcgc
     3901 cagcagcggg cgaacgtctt cttccggcgt aaaggcggtg agtaacgctt caatttcctg
     3961 cgcgctgcga cattcacacg cctgacgtgc cagttcccga cacgcctcgc tatccagttg
     4021 acgaagctgg cttttcaccg ccggaatgcg cgggctactc atactcagct catccaggcc
     4081 cagcccaagc agtagcggca gataacggct ttcaccgccc agttcaccgc aaatgcctac
     4141 ccatttgccc cgctgatgcg cagtggtaac tatttgctgc aacatgcgca ggaacgatgg
     4201 cgtaatcggg ttatatagcg gcgatacgcg cgggttatta cgatcgaccg catacagata
     4261 ctgggtcata tcgttggagc cgatactgaa gaaatcgacc tcatcgcaga agtgatcgat
     4321 gatgtagcac accgacggca cttccaccat gatacccagc gtaatcgtct ctgcatgacg
     4381 caggccatcg cgcttaagct caacgatcgc tttttggatc tcgcccttca cccataagat
     4441 ctgatcgagg ccgtggacca tcgggatcat caactgggcg ttgccgaaac tggcggcgcg
     4501 caaaatggcc cgcagttgag tgcggaacag gccagcaaat tccgggtaaa tacgtaccgc
     4561 gcgatagccg aggaacgggt tctcttcctg gggaatattc agataaggaa tgcttttatc
     4621 gcctccgatg tccattgtgc ggaagataat cggcttgtcg cccgctgcca gtagcacctg
     4681 ctggtaggct tcaaattgct cctgctcgtc cggctcgctg tcgcgatcca tatagagcat
     4741 ttcggtacgg aacagcccga cgccttccgc gccgttggca aacgcgccag gcgcttccag
     4801 agcggtgcca atattcgccg cgatatcaat acgcttgtta tcacgggaat aagccagctg
     4861 cgcggcagcc tgcgcctgtt gtttttggcg tttatccgcc agcgtctgcg cgacctgata
     4921 ataaccgctc accgcgtcat tcgggttaat cgccagcacg ccgcactggg cgtcaagcac
     4981 tgcaggttgc ccggcataac gcgcgatcgc atccagcggc aggccgctca gtaccgggat
     5041 cgccgaggcg cgggccagaa tcagggtatg cgaggtgcgc ccggttttct ccagaatcat
     5101 gcccgcaaga ttttttaaat ccaggcttaa aaactggctt ggggttaaat cctcggccac
     5161 cagaatagtc ggtttttcca gcaccagatt gttgcgcggc ttcagttccg gccaggtaat
     5221 atgcagcaac tgttcgctaa tatcgcgaat gtcgctaaca cgttcgcgca gataatcgct
     5281 ggcagaagca gaaagtttgg cgcaaacctg ctccatattt ctgatgatcg ccgcccccag
     5341 cccctgatgc tgttctgtca tcaggcgacg gatattgcct gcaaattcat catcctgaat
     5401 cagcgacaaa tgggcgctga ggatagtttt gctttcgccg tcacgctcac gcaattgctg
     5461 gttcagttgc tcggcaagcg ttgccaggct gtgctccagc cgggtggaat cttgcgcact
     5521 ggcggggatt gcccgataac tgtcgaggct gtcgctttgt aacagggtca gcgtacccac
     5581 gccgacgccg cttgccagca cattgccgta cagtaaatcc gggttaaggc ggcttaatga
     5641 acgcggcagc ggatgcgccg tcagttctgc ctgcgtaggc tgaacgctgt cgctgtcgat
     5701 aaagcgcacc tggatgtact cttccagcac gcgccgcgcc tgctcttcat cgctgccgct
     5761 aatgttcagg ctgcaactgt cattaaatag ggtgccggtg ccaatcagcg ccagcgagct
     5821 tttggcatcg gcctttgcgt tctggcgatg gttaataaaa gtgatttcgc tttgccactg
     5881 gctgcactgt tctttaagtt cccacgccgg acgagcatgt agaccgttag gcaaaggaca
     5941 gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc tgaaatccgt
     6001 cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa gttgctccat
     6061 ctgcaaaatg tcacggcaat cggcaatcga cgcggcgcaa tgtccgtggc gggtggcagg
     6121 ccccatcacc actgtgggta cgccagtacc ggttaagtgc accgctccac cgtccgtgcc
     6181 gccgttactg aacatatccg cctgtaacgg cacgccaatt tctgccgcca cggtttcgat
     6241 ccaggcggtg agttttggcg gcgcaatcag tgacttgtcg cttaacacca gcatcgggcc
     6301 gttaccaatc tggcgatggt tagccgcgcc ataattaaag tttttcgccc agcaggcggt
     6361 atcaaggaca atggcgacgt ccggcgacac cgcgcgggtg gcagtttgcc cgccgcgtaa
     6421 ccccacctct tcgctggaac tggccaccag ccacacttcc gcaggcagtt cagcgctgtg
     6481 tagttcgcgc agtaacgtca ccagcaggta gcaaccgagg cggtcatcaa aggctttccc
     6541 catcacccgc tggtggggga gaacctgaaa agtggtatcg aacgtgacgc gatcgcctgg
     6601 acgaattccc gcctgcatca cttcgtcata cgagcgcgcg ccaatatcca cgcgcatggc
     6661 gctgacatca ttcccctgcc ggtcgccgtc aagcaggcct ggaattttgc actcttcacg
     6721 ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgtacattgc caaccggcag
     6781 cacatcaatc gccccttcgc gggagatgct gcgcaccata aatcccactt cgtccatatg
     6841 cgcacagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca ccgatcccag
     6901 cccatcaaat cgcacttctt tttgcaggcg atccgcttct tccagcagga tctgccgcac
     6961 ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gctttcaata gcgataaatc
     7021 cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac ttctgcgcct
     7081 tgcggggtga ccagcacaac atcctcgatg cgcacgcccc cttgccctgg caaataaatc
     7141 cccggctcca cggtcagtaa catgcctggc tgtagcgtcg tggtgtcccg cggtgaaaaa
     7201 cgcggatctt catgaacttc aatgccgata gcatgagcgg tgttatgacc gaaatagtgg
     7261 ctaaaacctg cctcggtaat cacccgacgc gcggcttcgt caacctgctg gcagcgcacg
     7321 ccggggcgaa ttgcagagat tgctgcgagc tgtgcctgca aaacaatctg atagacgtta
     7381 aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt gcgcgtcata
     7441 tcagagcagt agccctgata gagcgcaccg aaatcgagag tgacaaactc gcccgctgca
     7501 acaatcttgt cgctggcttt gccgtgcggc agcgccccac gccagccact ggcgacaatg
     7561 gtgtcaaaag aggttttttc tgcgccctgc tggcgcataa accactccag ttcagcggct
     7621 atctcgcgct cgctcatccc cgcctgaata aagcggcgaa tatgctctgc accgcgatcg
     7681 gcaatcccac aggcaaggcg gattttctcc acctcctctg gcgttttgat ttgccgcagc
     7741 acatccggcg tggcgctaac cagtttcgca ttgagttcag actgccagcg atgcgcggtt
     7801 tcccaactca cctgctggcc ttcaaaaccg agcgtttgca actgctcatc ggcaatgatt
     7861 tgcctggcga tggttgtaag cgtgtgcgtc gcgtcaagca aatgcagctg gtaaccttgc
     7921 gtgcgggctt ctacatccgc gtaatagcgc gagtccacca gaatgtgcgc actttcacgg
     7981 ctaatcagca catagcctga tccggtggag atccccagat gcggctgttt gttctgccgt
     8041 gaggagagaa gcaccgcatc cagttgttgc gccttaagcc agtcgcgcag cgaagcgagt
     8101 aatgtcatct ggattttcct tcttacaggc tatcgattaa caatttgcct ttacggaaca
     8161 tcatcagacg caggaacacc accatcagcg cagttatgac tgcccccagc gcgatccccg
     8221 ccatatagac gcccaggtta gtgaccagcg gccatgccca gatagcggat tccgggaacc
     8281 attgcactgc gcccagccag acagcggcgg ttgagccgac aatcgcgccg accatatacg
     8341 acggaatggc ggtgatgggg ctttccagcg caaacggaat cgcaccctca ctgatcccca
     8401 taaaggcaag gaacatcgcg gtcttaccct gcggataaag ttgtgcgttg aacaggcgtt
     8461 tgccggttaa acgacggtca ataatggtcg ccagacccag accaatcggc ggaataacga
     8521 tagcgataga acgagcggtg accggtaata cgtggtcagt ggtaaagctg aaggcaacaa
     8581 aacctgcggc tttgttgatc gggccaccaa ggtcgattgc tgtcgccgcg gcgatcccca
     8641 ttgcgtacat cagcgcgccc ttctcacctg cggcagtcag cacagtacgg ataccgccgt
     8701 tgatccagcc cccaaagggg gtgatgacgt agtacatcgc cagcatgaca aaaatagcgg
     8761 aaagaatcgg tagcaaaaat gtggttttga acgctaacag gaaatccggt aactggattt
     8821 tctggttcat ccatttcaca aggtaaccgg caacgataga aataatcagc gcaccaatga
     8881 aggtggacgg caccggcgaa gaggtcgccc attgcatagt gctgggatcg aagttcagca
     8941 gttgcgtcgg ctgggtagac atcaagccgc cgataaaacc agccggaaac gccagtttgc
     9001 caccgataga gttcgccaca aaagcagcaa acatcggaat ggcaaaacca aacaacacgc
     9061 cgccgaagga ctgcgacaac caggcaaatt tcagtaagga gaggtcgaaa ccggagaatt
     9121 tcccgctatt aagagcatcc atgatgccga tctcagcagg aattttaagc cagctataag
     9181 caatcaactg gctgaaagcg aggatcacac cgcccataat taaggtcggc accatgcgtg
     9241 aaatccctga catcacatgc tgcgggagtt caccccagaa gctgctttta gaggcctgcg
     9301 gattcttaac tgccgctgcc gcaccggatg cgccaggcac aacggttgca ctgcgttttt
     9361 taatggccat aacaattatc cctgtcgaat attactgttg ttcagaggca atcatctctt
     9421 cgatttcttt gatgatgccc gcagcgtttt taattgcgtc ctgcaaagtg atttcataaa
     9481 cgtcgcgtga ttcgaaacgt tcgttatctt ccggggtaac tgccacggag tggatgatga
     9541 tggtcgcttc ggcgatatcc tgcgccgtca ggcgattctg gataccgtcc gcgccctggg
     9601 tttcaatttt cacttcataa ccggcttcta ccgccgcttc ttccagcgcc tgagcggcca
     9661 taaaagtgtg agccaggccc attgggcagg cacataaggc aatcagtttc ttactcatcg
     9721 ttaattcctc gttcagtgat agttgacgag tgaaattatg aacgtgcaaa acaaatcgcc
     9781 gttaccagac aaataatgca tttactggag aattaatccc gctgtacgga agtgtgacgc
     9841 aggtcggcct tgtgagggga cacaaagaaa agttgaatta actgtatgat ttaaaagatt
     9901 atcgggagag ttacctcccg atataacagg aaggatttac agaatgtgac ctaaggtctg
     9961 gcgtaaatgt gcaccggaac cgagaagacc cggattgtca tggacgatga gatacaccgg
    10021 aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac ggaaaccaga
    10081 ggctttgaag aactcaagga agcgcggcac gataccgccc gcaataaaca cgccgccaaa
    10141 tgtcccgaga ttgagcgcca gattgccgcc aaaacggccc ataatgacgc aaaacagcga
    10201 caacgcacgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa tatcttttgg
    10261 cttgagattt tctggcaggc ggttgtcggc tttaacaatt gcgcgataca aattcaccag
    10321 tcctgggcca gaaagcacgc gttccgccga aacatgacca atttccgcac gcaatatttc
    10381 gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc cttcgcccgg
    10441 caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc ccgttccggc
    10501 accgtaaacc gcaataggct taccttctac cggttctgcg ccaccaaact gaatcagatg
    10561 ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa taatttccag
    10621 atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg tatggttggt
    10681 cattgccacc cagtctccgg taatcgggca agcgatggca atacagccgt ctttcacctc
    10741 gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt aatcaagccc
    10801 tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag caagacgtgc
    10861 gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac tgctccgcta
    10921 aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac aacgccggaa
    10981 aggcaggctc cctgtaaata tcgatctggg tcacacaatt actttatcgt ttcagcacca
    11041 attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg cataaggaat
    11101 agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc gccgtggcaa
    11161 ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg gtattacaga
    11221 atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca cccctctgga
    11281 tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt ttcagccagg
    11341 gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccggtt ccgccctctg
    11401 cgctaccagg acttatcgta gcattaattc tcggtcttgc tggcggcgtc agcctggggc
    11461 cggaacatcc gatcatgacc gtcaatatcg cccttgcggt tgccattggc gctcgtctgt
    11521 taccgcgcgt caaccgaatg gagtggacta ttttagcctc tgccggaacc atcggcgcgc
    11581 tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat ggcagtagtg
    11641 aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct ggtgcactta
    11701 ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac ggacagatgg
    11761 aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca gcagggatgg
    11821 tcgccgtatg gtgcttacca cggttgcacg cgatgatgca tcaaatgaaa aatccggtgc
    11881 tcgtgctggg tattggcgga tttattctcg gtattctggg ggttattggt ggaccagttt
    11941 cgctgtttaa agggctggat gagatgcagc agatggtggc aaatcaggct ttcagcacca
    12001 gcgattactt tttgctggcg gtaattaaac ttgccgccct ggtcgttgct gccgccagtg
    12061 gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta gggttgatgc
    12121 tgcatgagca cgttcccgcc gtaccagcgg caataaccgt ttcctgcgct attctcggca
    12181 tcgtgctggt ggtaacacgc gatggctggt taagtctttt tatggcggca gtcgttgtac
    12241 ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg ctgttattag
    12301 caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac gataacgttt
    12361 agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg gctttacctc
    12421 cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga ataacaatgt
    12481 tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca gcaaacgcag
    12541 gcgaaatcac cctactgcca tcaatcaaat tacaaattgg cgatcgcgat cattacggta
    12601 attactggga cggtggtcac tggcgcgacc gtgactactg gcatcgcaat tatgaatggc
    12661 gtaaaaaccg ctggtggcgt catgataatg gctaccatcg tggctgggat aagcgtaaag
    12721 catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc ggaaaaggcc
    12781 gcggacatgg acaccgccat taaacacatt cgccaatgga gcacaaggcc tgatgcgacg
    12841 ctagtgcgtc ttatcaggcc tacaaaccgg ctctttaacg cgtagggcgg ataaggcgtt
    12901 tacgccgcat cgccaatagt gccagatgcg acgctcattc aactacaatc ccagcgccgt
    12961 ccccaccaat aaccagatat tcagcgccac gaccagcact acaatcaccc agcctgtctg
    13021 ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg tgaaaatcag
    13081 cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca taaccagaat
    13141 ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcagcatgg tgactgtacg
    13201 acgcacccac agcgggatat ggaagcgaat gaacccctgc atcaccacct gccctgccag
    13261 tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa agaccgttgc
    13321 cgcagcatgg cttaacagcg gttgcagcgt caaataagcc tcatcaagat cggcaacacc
    13381 agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca gattgacaaa
    13441 accggcaata gtcatggcga tagccacatc ccatttggtg gcggaataac gttgttgacg
    13501 cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat gcggcataat
    13561 cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta aactcgggat
    13621 caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact cgacaatgta
    13681 agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagcggtt tttgcccgcg
    13741 acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc cctgcaacaa
    13801 cgaaacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg ccaggtcggt
    13861 tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac gcggatagtg
    13921 atcgcgaatc tgctccgcca gatttttacc ggtggcaatc cctagtttgg cagagaggat
    13981 ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct gatagccgaa
    14041 gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac caatcgccgc
    14101 aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc cgctgctact
    14161 ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta tgtttcatgc
    14221 tatgccaaac gagaatgatt atcaaattca tttaaatggg ttgtggtgat ttctctgata
    14281 gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat gtttaatgtt
    14341 agcacattta cataactttc agcttccata cacaacatag cagaaatgta tgacagctca
    14401 ctatttttga agcttgtcac aggacgtcat tatagtgtgt gtcagatctc gttttcctta
    14461 accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga gcaaatatgg
    14521 accgcgtcct tcattttgta ctggcacttg ccgttgttgc gatcctcgca ctgctggtaa
    14581 gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt atcgaagtgt
    14641 tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa ggcttctccg
    14701 aaatgttcga aaaactgctc ggatttgcca acgaagggac taacttcgtc tttggtagca
    14761 tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc gtctttatct
    14821 ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc cgcgcaattg
    14881 gtttcctgct ctccaaagtc aacggcatgg gcaaactgga atcctttaac gccgtcagct
    14941 ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctt ggcaaaatct
    15001 cccgtaaccg tatgtacacc atggcagcaa cggcgatgtc caccgtttct atgtccatcg
    15061 ttggtgcata catgaccatg ctggaaccaa aatacgtcgt tgcagcgctg gtactgaaca
    15121 tgttcagcac ctttatcgtg ctgtcgctga tcaaccctta ccgtgttgat gcctgtgaag
    15181 aaaacattca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg ctgggtgaat
    15241 acattctggc aggtttcaaa gttgccatta tcgttgccgc aatgctgatt ggctttatcg
    15301 ccctgatcgc cgcgctgaac gcactgtttg ctaccgtgac tggctggatt ggctacagca
    15361 tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg atgggtgttc
    15421 cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt tccaacgagt
    15481 tcgttgcgat gatggatctt cagaaaattg cctctacact ctctccgcgt gctgaaggca
    15541 tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt atcgcaggtg
    15601 cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt ctgaagctgg
    15661 tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg gtgctgtaag
    15721 accatacata aaaaagccgg ggataattcc ccggcttttt tacgcctgtt aatcagctaa
    15781 tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct tatgcaatag
    15841 cgcctgttgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact tcgctttcgc
    15901 cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa tatctttcac
    15961 aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt cgtagttggc
    16021 atagccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca gctgctcgat
    16081 gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct caagaatgac
    16141 cgcctgcggg gagatgtgat aacgcttaaa tagacgaata atccgcccgg caatattctt
    16201 ttgcagcagc gtgagcggca ttaaattaac tgaaaagcgc gggccttttt tgtcgcaagg
    16261 gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac gcgcgctaag
    16321 gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc cgtcatattt
    16381 cagtcgcgcg aggacctcat cataaccttc accctctttg ttgcgaattg gctgggcgta
    16441 gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata gcacctgttt
    16501 agtggtctgc ccggaaacca tctcctctcg gctatccagc gccagcacat gatgatgtgc
    16561 gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg tttcctgatt
    16621 tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat tgttccagtg
    16681 aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg tttcaggccc
    16741 gctcagcact aacagcagtt cactacccgg caattgatac aacttttcgt tttcctgcat
    16801 caatggcagc agcgtacggt aaattgatcg gatacagtga acacgcatca tcaacccgta
    16861 atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaaactct tgcccgcctc
    16921 ttgctccggc gcttgttcca acgcacgaaa gttgggcagg agcgttaacg gatcggtcag
    16981 cgcctgcaaa tgccagcggc gattaagcca ttcactgcga tgataaatgc gcaccatata
    17041 gagcaggcaa acgctaaagg aaatcagcac cgcgagaata aatgccagcg aatattcggt
    17101 ttccacccct tgcaaaaagt tctggttgta attcagcagg caaagcgttg aaacagccca
    17161 agtgagattt aaaaacggat agcgaagctt accgaccccg agggtaaaga tgatgaagaa
    17221 aacgggtacc aggtaaccgg caataaagtc gttttcataa ggtgtgcaca acagaagtag
    17281 cagcacgctt aacgctgcca gccagcttaa ggtaaatgcg cgtttctctt tgcccaacga
    17341 cggagcgata tccctgcgcc acaatatctg cgcaaagtgg ggacttacaa tcatgcgagt
    17401 gagatagtag aaaagcatgt tgtaaatcag cacagcggtg aaaaggctta gcaaatcaac
    17461 gaccgtgaaa atggcatccg catcgccgaa aaaggtggat atctttagcg ggaaatcaaa
    17521 gaagctcccc acaagataca tgctgcattt gatgccgatc ggcgtcacca aaccaagcca
    17581 gaacagacgt tgccagacat atcggctggt caatccataa cgccaacgcg tccccagctg
    17641 ccagcgtaat attgcgcagg cacacaacac gacaaacgtc tggctaccga gcatgaccgc
    17701 agtctgtaaa aaagagagat gaaagttcca cagattggtg caaaacatcc cgcataaaac
    17761 gggaacgaca ccgcgccagc caaagataaa caagattgac agcattacgc acagtggcat
    17821 ccacgccaga aagatataac tggaatggat aatggcaagt ggcgaaataa aacgggatag
    17881 ctgaataagt accacggtga gcgtaaacgc tagtgtgaat atcttgatat tttttatcag
    17941 gttatgctcc acaaacatga gtacactact ctcactgatc ggggttttca ggcacacaaa
    18001 taataagttt attcgcattg cgaagcaaac aggggccatc ctgttgccga tttttcatcc
    18061 aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa aagcggcaaa
    18121 aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg gagctaagcg
    18181 ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga gctatagccc
    18241 cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct aagcgggatc
    18301 gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat agccccgtca
    18361 cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg
    18421 caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa
    18481 ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgc tataaaatga
    18541 actactaata gacccacata cattcaggga attgttatgt tcaaggagag gatgacacca
    18601 gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg ggtacgcaag
    18661 gaaggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg actggttcac
    18721 gtcaatgaac aggttcgtga atatattcgt aatgctgaac gtccagaagg tcagggagaa
    18781 gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact ggcgaaagag
    18841 atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg gattatcgga
    18901 ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac gcaataaaat
    18961 gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta accgttggat
    19021 cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact
    19081 tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct tccataacac
    19141 accgatgctt ctggaagccg aagaacccct tgccgaatac gcaccgggga ttcgcactcc
    19201 tgcttatcgg caaatcatca gtgcaataga caacatgact cacagtgagc aggaaaaagt
    19261 tgcgcaattt ttgtcacgcg aaggaattcg taatttcctt gctcgtctcg acatagatga
    19321 atcagcttga catcctccac gccctgaatg acgaggatcc ctgctacgtt caggctgtcg
    19381 cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc atctctccac
    19441 aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc gccgtttaca tcggcgttcg
    19501 ctgtatatcc acatacctgg catctgaatt tactttgtga caggcggttt tctttcgctg
    19561 tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagcc agtacctgac
    19621 cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag ccctgatcca
    19681 gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg ctgaccgtac
    19741 ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc attgcgtggt
    19801 ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct gcggatattt gcggtacagg
    19861 aatgcagtcg ctgtattttg cgtttctgct tctgccagtt attgctgaat ttgaccttgc
    19921 ggcttaactg tcgctgaagt ctcgccaact tcttctggtt tttctgaaaa ctgtttacag
    19981 gctcaaagac tgtgccatct gacagcgtgg cgagtttagc cacgccagca tccagtccga
    20041 ccattgatgc tgaagggtga accggagttg atacttcact ttctgtctga atactgatgt
    20101 accacttacc gcaggactgg ctgacagtga catttttcac aacacccgtg acctgacggc
    20161 tgttcaggta gcgcatccag ccaagtttcg gcagaaaaat acggctgttt tcctgatcga
    20221 gcttaacgcc ctgcgggtag cggaatgcat cattctgtcc ccgctttttg aatcggggaa
    20281 aagcagcccg cttccggaag aagtttttgt aagcccgctc aagctctttc agtgactgtt
    20341 gcaatggctg tgacggggca tctttaagcc attgcgtttc agtggcgttt ttccactcaa
    20401 ccagccagga agccattttc ccgtaaggga tgtatttatt accggcctca tgattctcat
    20461 tctgaagcgc cagagcacga ttgaaaacga aacgacatgc gcctgcgaag cgcctcattt
    20521 cacgctcctg ttgaccacct ggtcttaact ggaatttaaa tgcctgtagt cgtttcacag
    20581 gccaaatgat actttggtct atgaataaag aaaccgatat ccgacgggga aggcattgtg
    20641 ttttcctgat gcatgttcat ctgttctttg tcaccagata ccgacgtcag atttttgact
    20701 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
    20761 aactggttga aatggatggc gaaccagatc acgtccattt gttaactaac tatcctccca
    20821 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg ttactgcgac
    20881 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtcctggct
    20941 attttgccag tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
    21001 agcaaacacc tggtcaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt
    21061 ttacggcgca ccggataaat aaaaaacggc aggggtatct ctcctgccgt ttatctttta
    21121 tgcgctaatt actgctgatt ttcgcgttcc gcaataaaag ccagcgcttt gttgatacgc
    21181 tcgatactgc gggtcttacc gatcgcgtga acggtaacgt ccagcgccgg agactgcccc
    21241 gcaccggtta cggcgacacg cagcggcata ccaactttac ccatacccac ttccagctca
    21301 tccgccgtcg cctgaatagc gtgatgaacg ttttcagctg tccagtcagt aatcgcggcc
    21361 agtttgtcac gaaccacttc cagcggctga cgcgctaccg gacgcagatg ttttttcgcg
    21421 gcgtcggcat cgaactcagc aaaatcttcg tagaaataac ggcagctctg tgccatctct
    21481 ttcagcgtct tgcagcgttc gcccagcagt ttcaccagat cagccagctg cgggccgtta
    21541 cgggtatcga tattttcctg ctcaatgtgc cactgtaagt gagtagcaac atactccggc
    21601 ggcagcgcgt taatgtagtg atggttcagc cacagcagct tgtcggtgtt gaacgcactg
    21661 gcagatttgc tgacggcatt caaagtgaag tatttgatca tctcttcacg agtgaagatt
    21721 tcctgatcgc cgtgggacca gcccagacgc accagatagt tcagcagtgc ttctggcaaa
    21781 taaccgtcat cacgatactg cattacgctg actgccccgt gacgtttgga cagttttttg
    21841 ccgtcatcgc cgttgatcat agaaacgtgc gcgtaaaccg gtaccggcgc tttcagggct
    21901 ttaagaatgt tgatctggcg tggcgtgttg ttgatatggt cttcgccacg gataacgtgg
    21961 gtgatttcca tatcccagtc atcgacaacc acacagaagt tataggttgg ggaaccatcg
    22021 gtacggcgga taataagatc gtccagttcc tggttgctga actcgatcgg accacggatc
    22081 tgatcgtcaa aaacaacaga accttcctgc gggttagcaa aacgtacgac acacggctca
    22141 tcatcagcgt gatgctcatg actgtggcgg cagcgaccgt cataacgcgg cttctcacct
    22201 ttcgccattt gctcttcgcg cagcgcttcc aggcgctctt tagagcaata gcatttataa
    22261 gctgtgccct cttccagcat ctgatcgatc accgcgttgt agcgatcaaa acgtttggtc
    22321 tggaagtacg gaccttcatc ccactccagg ctcagccagt tcatgccatc cataatggct
    22381 tcgatagctt ccggcgtgga acgctcaaga tcggtgtctt caatacgcag cacgaactca
    22441 ccgccgtgat tacgtgcaaa aagccaggag taaagagcag tacgcgcgcc gccaacgtgc
    22501 agatagcctg ttgggcttgg cgcgaagcga gttttgattt tcatgaaatg gccttacgtt
    22561 tagaaagatg ccgacaaccg gcaaatcctg gaaaaattga gtgggcgata ttctatcact
    22621 cccgcctgat tcctcaatgt agttcgggtt gcaaccttac gcattattgt tttcgttgac
    22681 aaattgcgca acacggtctg ttttgcgatc atcttgctta aatttacgac gaacgaacaa
    22741 tttctttaga aaatgcgttg actcattttg aactctccct ataatgcgac tccacacagc
    22801 gggggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga
    22861 tcccgtcatc acccaccaac tactttatgt agtctccgcc gtgtagcaag aaattgagaa
    22921 gtgggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga
    22981 tcccgtcatc acccaccact ttctcaccag ctaaatttct tgtaaaaatg tgaagtaccg
    23041 aagtgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    23101 gatcccgtca tcacccacca cttcgggtcg ttagctcagt tggtagagca gttgactttt
    23161 aatcaattgg tcgcaggttc gaatcctgca cgacccacca atgtaaaaaa gcgccctaaa
    23221 ggcgcttttt tgctatctgc gatttgtaag attgattttt cttctcaagt tccttgttct
    23281 tctgccgata ttacctcgta cagtgacgag gactgaacaa tggccactat ctcttcaact
    23341 tcaattcctt ccatacaaac acagagtagt aacagggcat cacaaggtag cgatgttgct
    23401 tcacaaatcg cgcggataag tcagcaaatc atcaaactca ctcaacacat taaagagatt
    23461 gtagacacta gcggcagtgc tgaagacaag caaaaacagg ccgaattgat tcaacagcaa
    23521 atcaccctac tggaaacaca actggcgcag ttgcagagac agcaggcaga aaaggcgcag
    23581 gagaaagagc agcgtttgtc tctgaatgta tcccttctaa atcctgttga aaatacgact
    23641 catatcggta tctatatctg atacaacgga tccccttccc gcccagccat tagcgtattg
    23701 ccctggtctc gtaccacgtc ccataacgct tcagcggcgg tggaaagtga acggtttttc
    23761 ctacgcacca gcatcaactg gcgttccacc accggcgtaa tgcgctttac caccagcggg
    23821 ctaccttcag gcaatggtag cgccagtgcg ggaagaatac tgatgccgat gcctgcggct
    23881 accatcggga acagcgtcgc cggatgtcca atctcctgca caatattcgc ctgaatacca
    23941 ttgcgcgcca gcgctgcgtc aatcagcggt cggctgcctg acgcataatc ctgcaacacc
    24001 aatttcgctc cctgtaatgc ctgccagggt acataatctt ccgcggccaa agcgctatcc
    24061 cgatggcaaa gcagaaagaa aggctcggaa agaatcactt cacattgcag atcgcccact
    24121 gggcctggat cgatgacgat gccaaagtcg acatctccct ggcgaatact ttccatcacc
    24181 cactgctgcg gacgatcgtg caggacaaac tgaatatctg gatagcggcg atggctttca
    24241 gcaatgcatt gcggaataag atgcgcggaa atggtctggc tggcagcgac ccgaactttt
    24301 ccgctcagtt gttgccccat acgcccggta tcgcgcaacg tgctgttaag ttcatccagc
    24361 agtcgctcaa gacgcaaagc caactgctgc cctgcatctg taagcaccac ttcacgcgtg
    24421 gttctgtcca gcaggcgaac accagtatga ttttccagct ccttcacact gtgacttact
    24481 gccgactggc tcaggccgat acgctctcct gcacgactaa aacttttctc ctgcgctact
    24541 gtgacgaaaa cctttaattg ttttaaagaa taattcatct gttttcttca tgaatggatg
    24601 caataaatca attttatttc tcaaactgga agaagcacaa tagaaccatc gatcatctgg
    24661 agtctttatg aaactttttc gtatcctcga tcctttcacc ttaaccctga tcacggtggt
    24721 gttgctggcc tctttctttc ctgccagagg cgatttcgtc cccttctttg aaaatctgac
    24781 caccgcagct attgccctgc tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat
    24841 tattgctggc ggtggtcact ggcgactgca tttgtgggta atgtgcagca ccttcgtgct
    24901 gtttccgatt ctgggtgtac tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct
    24961 ctactccggt tttctctact tgtgcattct cccggctacc gtgcagtctg caatcgcctt
    25021 cacgtcaatg gcgggcggta acgtcgcggc ggcggtttgt tctgcgtcag catccagtct
    25081 gctggggatt ttcctttcac cattgctggt tggtctggtg atgaatgttc acggtgcagg
    25141 gggcagcctt gagcaggtcg gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg
    25201 gcatctttcc cggccgtgga ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa
    25261 aactgaccag acgtccattc tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa
    25321 tggtatctgg cataaagttg gctggggatc attgctgttt atcgtggtgg tcagctgcgt
    25381 tcttctggct atcgtgattg tagttaacgt ctttatggca cgccgactgg gcttcaataa
    25441 ggcagatgaa attactatcg tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc
    25501 gatggcaaac attctgttcc ccacatcggt gatcggtatg atggtgctgc ccctgatgat
    25561 tttccatcag atccaattga tggtctgtgc ggtgctggcg cgtcgataca aacgccagac
    25621 cgaacagtta caggcgcagc aggaaagcag cgccgataaa gcttaaagcg gacgcttcag
    25681 gggctggacc agttgcgtca gcccctcggt tttaatcagc agcgtgattt gcattagctc
    25741 accgagcttc cctgccggga actcatcttt gcgggcaaac cacagcagat actcctccgg
    25801 caggtcgatt aagcgacgcc ctttgtattt gccaaacggc attatcgtat tggctatttc
    25861 aatcagctgc tctttttcca tctcagctac ccagcaaacg cagcatttcc gcttcgtcga
    25921 tgacttcaat gcccagttcc tgcgccttcg ccagtttaga tcctgcagct tcacccgcta
    25981 tcaccagatc ggttttcttc gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc
    26041 gagctttagc gtcatcacgc gacatctggc ttaagctgcc cgtaagcacc acggttttac
    26101 cagcaaacgg gctgtcaatc tcttccgcgt tgataacgat cggcgcaggc cagtgaacac
    26161 cttccgccaa cagctcgctg atgacattgc ggttgctttc ttcggcaaag aagttgtgaa
    26221 cgtgggatgc aacgacaatg ccaacatcag gcaccttttg cagctcttca atcgaagcgg
    26281 cttccagcgc ttccagcgtt ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga
    26341 cttcacggat gccaagtgca tagaggaagc gagcaaaggt ggtttctttc gctttttcca
    26401 gcgcgttaac cacgttttgt gccgattttg gccccatacg ctccagtccg gtcagtttgc
    26461 ctgcggtgag tttgaacaga tccgccggag tgtggacata ttctttttca accagctgat
    26521 cgatgatttt gtcgcccatt ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca
    26581 gcgactcttt acgctgcgca ccgcaaatca gaccaccagt acagcgggca accgcttcac
    26641 cttccacacg ctcaacgtca gaaccacata ccggacaata cgtcgggaat acaacctcac
    26701 gggtatcttc cgggcgttca gaaagcacga cgttaaccac ctgcgggatc acgtcgccag
    26761 cgcggcgaat caccacttta tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat
    26821 tgtgtaaggt tgcgttactc accagcacgc ctgcaacatg gacaggttcc agacgcgcaa
    26881 caggcgtaat cgcgccagta cgcccaacct gaaactcgac gtcacgcaca aaggtcatct
    26941 gttcctgcgc cgggaattta aacgctaccg cccagcgcgg ggctcgcgcg acaaagccaa
    27001 gctgctcctg ctgttccagt gagttgacct taatcaccac gccgtcgata tcaaagccca
    27061 gcgtcgggcg gtcttcttcc actttgtgat agaacgccag cacttcttcc gccgattcac
    27121 aaagcgttac ccgatcgctg accggcaacc cccacttttt aaattgcagt aaacggccaa
    27181 gatgagtatc cggcagctcg ccaccttcca gaacaccaac gccatagcag aaaaaagtga
    27241 gcggtcgctt cgctgtaata cgcggatcaa gctgacgcag tgaaccagct gccgcattac
    27301 gtgggttagc aaacactttc ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc
    27361 ccgcctgcgg caggaacact tcaccacgca cttccagacg cgccgggata ttctctccgt
    27421 gcaacttcag tggaatggcg cgaatagtac gcacattaga cgtgatatct tccccggtgg
    27481 tgccatcgcc acgagtcgcg gcactgacta aaacgccatt ttcatacaga atactgacgg
    27541 caaggccatc cagcttcagc tcacagcacc aggtgacttt ctcgttgctt ttcagacggt
    27601 cctgcacacg tttgttgaag gcaagaaagc tttcttcatc aaaaacgtta tccagcgaca
    27661 gcattggcac ttcatggcga atctggctga aagccgccag cggcgcagca cctacacgtt
    27721 gggtaggcga atcaggcgta atcagttctg gatgtttggt ttccagctcg cgcagttcgc
    27781 gcatcagcct gtcgtattca gcgtcgggaa tttccggcgc atccatcaca tgataaagat
    27841 attcatgatg gcgaagcgtc gttcgcagtt ctgtcagttg ttgttcgatt gattccatat
    27901 cgcaccatca atgctaaaaa cccccgacaa gcgggggttc gaagaggagt taatttgcct
    27961 taagtgtatc aggcgttggc gtctttaact tcgcggatga tgtcctggta ctcgcgcaat
    28021 ttctgcggag tcatcatacg gcgctgatcg tcaagcacga caccgcccac ttcatcggca
    28081 atatgctgcg cagattgcag catcagcttg aagttctgca gctcgtcacc gtaagacggt
    28141 acctgcataa agatagtgac acccggagta gtgaaatcct tcatttcagg atcaaaggtt
    28201 cccggtttca ccatattcgc caggctaaat aacgccgggc cgctgccatc cgggctaaga
    28261 tgacgatggt aaatattcat atcgccaaaa atgaagcccg cttgttgaat gctgttaaga
    28321 agcagttcac cgtttagctc gctaccgtga tgcgccgcga cgttcatgat aatcaccgct
    28381 tctttgcgct tcggtttatc cataactgga gccggttccg ctacaggctc aggctgtggt
    28441 gccgctacgg gttctgcagg ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcact
    28501 ggctgcggcg ctggcgcgac ctgtggcgaa actggctgct gcaacggctg ttcaggctgc
    28561 ggctgatagg caggctgctg cacgggttgc tgcaccggct gcgctggacg cggagcatgt
    28621 tgcggcggta cctgcgcttc aggcggctgc tggaccggtt gacgcggctg cgcagacgca
    28681 taaggcggtt ggtactggtg ttgcggcgac ggacgagcag cctcatgctc ctgggcgtta
    28741 gccggggcat gattcacgcg gtgaacacga acctcaccaa cgccctcatc atcttcgaca
    28801 tcctcgtcat aagaatcgtc gtcacgtttt gacttcattc gttttaatgg ccgatcgcgg
    28861 aacatagaag atcgttcttt acggctggtc cagaaaccat gtaccagtaa agcgattatg
    28921 gcgatcgcgc caacaatgat taatatcaga cgcaaatcct gcatcattat attctctgtt
    28981 gttctaacac cttgccacca cggcaaacat ttactcacta agagtatttg ccgattacct
    29041 caagtgcaag tgcactatta actttcacag cacaaagata gatgaaatcg tgctttttgc
    29101 tgttttttcg aacatatcct aactgtccat tgcgcaatta cccggtaaaa tacgcacaat
    29161 tttcctggga ttggtcaaaa ggagctcatc ctgattatgg tttcatcatt cacatctgcc
    29221 ccacgcagcg gtttttacta ttttgcgcaa ggctggaagc tcgtctcgca acctgggatt
    29281 cggcgtttcg ttattttacc gctgctggtc aatattttgt tgatgggggg cgcattctgg
    29341 tggctcttta cgcaactcga tgtctggatc ccgactctca tgagttatgt tccggactgg
    29401 ttacaatggc tgagctatct gttgtggcct ctggctgtca tctctgtgct gttagtgttt
    29461 ggctatttct tctccacgat tgctaactgg attgccgctc cgtttaacgg tttattggct
    29521 gaacaactgg aagcacgatt gactggcgct acaccgccag ataccgggat tttcggtatc
    29581 atgaaagatg tgccgcgaat catgaaacgc gaatggcaaa aatttgcctg gtatctgcca
    29641 cgcgcaattg tattgctaat tctttacttc atccctggta ttgggcaaac cgtcgcgccg
    29701 gtactgtggt tcctgtttag cgcctggatg ttagccatcc agtattgcga ttaccccttc
    29761 gataaccaca aagtgccgtt taaagagatg cgcaccgccc tgcgcacacg caaaatcacc
    29821 aatatgcagt ttggtgcttt aaccagtctg tttacgatga tcccgctgct taatctgttc
    29881 attatgcccg ttgccgtttg tggcgcgacg gcgatgtggg tcgattgcta tcgcgataaa
    29941 cacgcgatgt ggcggtaaca atctaccggt tattttgtaa accgtttgtg tgaaacaggg
    30001 ggggcttatg ccgcccctta ttccatcttg catgtcatta tttcccttct gcatatagat
    30061 atgctaaatc cttacttccg catattctct gagcgggtat gctacctgtt gtatcccaat
    30121 ttcatacagt taaggacagg ccatgagtaa gatttttgaa gataactcgc tgactatcgg
    30181 tcacacgccg ctggttcgcc tgaatcgcat cggtaacgga cgcattctgg cgaaggtgga
    30241 atctcgtaac cccagcttca gcgttaagtg ccgtatcggt gccaacatga tttgggatgc
    30301 cgaaaagcgc ggcgtgctga aaccaggcgt tgaactggtt gaaccaacca gcggtaatac
    30361 cgggattgca ctggcctatg tagctgccgc tcgcggttac aaactcaccc tgaccatgcc
    30421 agaaaccatg agtattgaac gccgcaagct gctgaaagcg ttaggtgcaa acctggtgct
    30481 gaccgaaggt gctaaaggca tgaaaggcgc aatccaaaaa gcagaagaaa ttgtcgccag
    30541 caatccagag aaatacctgc tgctgcaaca attcagcaat ccggcaaacc ctgaaattca
    30601 cgaaaagacc accggtccgg agatctggga agataccgac ggtcaggttg atgtatttat
    30661 tgctggcgtt gggactggcg gtacgctgac tggcgtcagc cgctacatta aaggcaccaa
    30721 aggtaagacc gatcttatct ctgtcgccgt tgagccaacc gattctccag ttatcgccca
    30781 ggcgctggca ggtgaagaga ttaaacctgg cccgcataaa attcagggta ttggcgctgg
    30841 ttttatcccg gctaacctcg atctcaagct ggtcgataaa gtcattggca tcaccaatga
    30901 agaagcgatt tctaccgcgc gtcgtctgat ggaagaagaa ggtattcttg caggtatctc
    30961 ttctggagca gctgttgctg cggcgttgaa actacaagaa gatgaaagct ttaccaacaa
    31021 gaatattgtg gttattctac cgtcatcggg tgagcgttat ttaagcaccg cattgtttgc
    31081 cgatctcttc actgagaaag aattgcaaca gtaatgccag cttgttaaaa atgcgtaaaa
    31141 aagcaccttt tcaggtgctt ttttgtggcc tgcttcaaac tttcgcccct cctggcattg
    31201 attcagcctg tcggaactgg tatttaacca gactaattat tttgatgcgc gaaattaatc
    31261 gttacaggaa aagccaaagc tgaatcgatt ttatgatttg gttcaattct tcctttagcg
    31321 gcataatgtt taatgacgta cgaaacgtca gcggtcaaca cccgccagca atggactgta
    31381 ttgcgctctt cgtgcgtcgc gtctgttaaa aactggcgct aacaatacag gctaaagtcg
    31441 aaccgccagg ctagacttta gttccacaac actaaaccta taagttgggg aaatacaatg
    31501 ttccagcaag aagttaccat taccgctccg aacggtctgc acacccgccc tgctgcccag
    31561 tttgtaaaag aagctaaggg cttcacttct gaaattactg tgacttccaa cggcaaaagc
    31621 gccagcgcga aaagcctgtt taaactgcag actctgggcc tgactcaagg tacagttgtg
    31681 actatctccg cagaaggcga agacgagcag aaagcggttg aacatctggt taaactgatg
    31741 gcggaactcg agtaatttcc cgggttcttt taaaaatcag tcacaagtaa ggtagggtta
    31801 tgatttcagg cattttagca tccccgggta tcgctttcgg taaagctctg cttctgaaag
    31861 aagacgaaat tgtcattgac cggaaaaaaa tttctgccga ccaggttgat caggaagttg
    31921 aacgttttct gagcggtcgt gccaaggcat cagcccagct ggaaacgatc aaaacgaaag
    31981 ctggtgaaac gttcggtgaa gaaaaagaag ccatctttga agggcatatt atgctgctcg
    32041 aagatgagga gctggagcag gaaatcatag ccctgattaa agataaacac atgacagctg
    32101 acgcagctgc tcatgaagtt atcgaaggtc aggcttctgc cctggaagag ctggatgatg
    32161 aatacctgaa agaacgtgcg gctgacgtac gtgatatcgg taagcgcctg ctgcgcaaca
    32221 tcctgggcct gaagattatc gacctgagcg ccattcagga tgaagtcatt ctggttgccg
    32281 ctgacctgac accgtccgaa accgcacagc tgaacctgaa gaaggtgctg ggtttcatca
    32341 ccgacgcggg tggccgtact tcccacacct ctatcatggc gcgttctctg gaactgcctg
    32401 ctatcgtggg taccggtagc gtcacctctc aggtgaaaaa tgacgactat ctgattctgg
    32461 atgccgtaaa taatcaggtt tacgtcaatc caaccaacga agttattgat aaaatgcgcg
    32521 ctgttcagga gcaagtggct tctgaaaaag cagagcttgc taaactgaaa gatctgccag
    32581 ctattacgct ggacggtcac caagtagaag tatgcgctaa cattggtacg gttcgtgacg
    32641 ttgaaggtgc agagcgtaac ggcgctgaag gcgttggtct gtatcgtact gagttcctgt
    32701 tcatggaccg cgacgcgctg cccactgaag aagaacagtt tgctgcttac aaagcagtgg
    32761 ctgaagcgtg tggctcgcag gcggttatcg ttcgtaccat ggacatcggc ggcgacaaag
    32821 agctgccata catgaacttc ccgaaagaag agaacccgtt cctcggctgg cgcgctatcc
    32881 gtatcgcaat ggatcgtaaa gagatcctgc gcgatcagct ccgcgctatc ctgcgtgcct
    32941 cggctttcgg taaattgcgc attatgttcc cgatgatcat ctctgttgaa gaagtgcgtg
    33001 cactgcgcaa agagatcgaa atctacaaac aggaactgcg cgacgaaggt aaagcgtttg
    33061 acgagtcaat tgaaatcggc gtaatggtgg aaacaccggc tgccgcaaca attgcacgtc
    33121 atttagccaa agaagttgat ttctttagta tcggcaccaa tgatttaacg cagtacactc
    33181 tggcagttga ccgtggtaat gatatgattt cacaccttta ccagccaatg tcaccgtccg
    33241 tgctgaactt gatcaagcaa gttattgatg cttctcatgc tgaaggcaaa tggactggca
    33301 tgtgtggtga gcttgctggc gatgaacgtg ctacacttct gttgctgggg atgggtctgg
    33361 acgaattctc tatgagcgcc atttctatcc cgcgcattaa gaagattatc cgtaacacga
    33421 acttcgaaga tgcgaaggtg ttagcagagc aggctcttgc tcaaccgaca acggacgagt
    33481 taatgacgct ggttaacaag ttcattgaag aaaaaacaat ctgctaatcc acgagatgcg
    33541 gcccaattta ctgcttagga gaagatcatg ggtttgttcg ataaactgaa atctctggtt
    33601 tccgacgaca agaaggatac cggaactatt gagatcattg ctccgctctc tggcgagatc
    33661 gtcaatatcg aagacgtgcc ggatgtcgtt tttgcggaaa aaatcgttgg tgatggtatt
    33721 gctatcaaac caacgggtaa caaaatggtc gcgcctgtag acggcaccat tggtaaaatc
    33781 tttgaaacca accacgcatt ctctatcgaa tctgatagcg gcgttgaact gttcgtccac
    33841 ttcggtatcg acaccgttga actgaaaggc gaaggcttca agcgtattgc tgaagaaggt
    33901 cagcgcgtga aagttggcga tactgtcatt gaatttgatc tgccgctgct ggaagagaaa
    33961 gccaagtcta ccctgactcc ggttgttatc tccaacatgg acgaaatcaa agaactgatc
    34021 aaactgtccg gtagcgtgac tgtgggtgaa accccggtta tccgcatcaa gaagtaattc
    34081 ttgctgcagt gaaaaatggc gcccatcggc gccatttttt tatgcttccg ccagcggcgg
    34141 caaaatcaat tcatcgctct catgctgctg ggtgtagcgc atcacttcca gtacgcgcaa
    34201 ccccgcgcgg tgcaccgcat cgtttaatgc cttccctttc agcaaaccac tgataagctg
    34261 agcacaaaac aggtcgccag tccctttcag gtcggttttt acccttgaat gggaaatgac
    34321 attcacgctg tcggcagtga ccaccacaac cagcatctcc tgattttctt cattaccgga
    34381 ggcgctggta atcaccaccc attttagtgt gtctgaaagc agactttttg cggcagcaat
    34441 ggcactgtcg agatcgcggc aatttttacc ggtcaggatt tccaactcaa agatattggg
    34501 ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac gcttcgggaa ggtcaggttt
    34561 gacataaatt ccgctgtcaa tatcgccaat caccggatcg accatgatca ataggtcagg
    34621 atggtctttg cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg atgccgttcc
    34681 catatagccc gtggttacag cacgaagttg gcgcagcgca tcacgctcct gaagtgcacg
    34741 caaatagccg ctaaaccatt catccggaat cgcaccaccg tagaaagtgt cataatgcgg
    34801 cgtattgctc agcaataccg tcggcacggc aaagacattc aggccgttct gtttgatagc
    34861 aggcacggca atgctgttgc ccacgctgcc gtaaaccacc tgcgactgca cggcgacgat
    34921 atccgcctgc agcgccctgc tcttatcgtt aaacaacaac aaactactca tttaattttt
    34981 tctccttgcc gatgatcctc atcgtattcc aaccgaaact ttacctgatt ctggcagtca
    35041 aatcggctat tacaaaacaa ggataaggta attcagtgaa gaaaatcatt tgtctggtca
    35101 ttacactact aatgacactc cccgcttacg cgaagttaac tgcccatgaa gaagcccgca
    35161 tcaacgccat gctggaggga ttagcacaga aaaaggattt gatatttgtg cgcaacggtg
    35221 atgaacatac ctgcgatgaa gcggtttctc atctgcgtct gaagctgggc aatacccgta
    35281 accgcattga cactgccgag cagtttattg ataaggttgc ttcgtcgtca tcgattaccg
    35341 ggaagccgta tattgtgaag atccccggta agagcgatga gaatgcacag cctttttaca
    35401 tgcgttaatt gcgcagacgg ataaaacggt gcctgcgcaa taatcttaaa tccccgcccc
    35461 ctgactaaaa tgctcttccc caaacacccc ggtggaaagg tagcgatcgc cacgatcgca
    35521 gatgatcgct accaccaccg caccagggtt agcttttgcc acccgcagtg ctccggcaac
    35581 cgcgccgccg gagctgacgc cacagaatat tccttcccgc accgccagtt cgcgcatggt
    35641 gttttccgca tcgcgctgat gaatatccag cacctcatct accagagaag cgttgaaaat
    35701 ccccggcaga tattccgcag gccagcggcg aatgccggga atgctactgc cctcttccgg
    35761 ctgcaggcca acaatggtca ccggtttgga ttgttcgcgc ataaagcgtg aaacgccggt
    35821 gatagtgccg gttgttccca tgctggagac aaaataggta atgcgcccgc cagtctgctg
    35881 ccagatttcc ggcccggtag tggtgtaatg ggcgtaaggg ttatcgggat tattgaactg
    35941 atcgagcagc tttccttcac cacgatccgc catctccagc gccagatcgc gcgcaccttc
    36001 catgccctgc tctttggtga ccagaatcag ttccgcacca taagcacgca tcgccgcacg
    36061 gcgttcctgg ctcatgttgt cgggcatcag caatttcatg cgatagcctt taagcgcggc
    36121 aatcattgcc agcgcaatgc cggtgttacc actggtggct tcgattaaca catcacccgg
    36181 tttaatttcc ccgcgctttt ccgcctcgac gatcatcgaa agcgccgcac gatcttttac
    36241 cgaacctgcc gggttattgc cttccagttt taaccacact tcactgccgt tattcggccc
    36301 cattcgctgc aacttcacca gaggcgtatt gcctattgtt tgttctaatg tactcacgat
    36361 ctctatccat acgtggtgtt gcctgatgcg acgcttgcgc gtcttatcag gcctacaggt
    36421 tacaaacctt gccattaaaa agcccggatc gcgggaagcg cctccgggcg tttaacattc
    36481 actcaaccta tcaggcgctt tgtgcgagag caagttcctc atctcggggt tcgatacgct
    36541 cgtcgccgtt atacagccgc gcatgttgca gaccaacgaa taaacgctcg ccacgctgcg
    36601 gggcatcgtc gccatgcatc acgaccgtca gcggttcgtt gtaccacccc agcggctgca
    36661 ccactaattg ggtgtagtga cctttcgggc tggcttccag tacctgtacc ggcagcggcg
    36721 aatcgaggct ggtacggcgg ctgatatcca cttcccacgg gcgcaggaag agatccaccg
    36781 gcccctgata cgcaggtgtg tagcccagcg gccagcgatg tgcgccaaca tggaactgcc
    36841 cgccgcgaat ggttccctgc aggcggttca cttcgcccat aaattcgagc acaaaacggg
    36901 tcgccggttc gcgccatacc tgatccggcg cgtcagcctg ttcaatattg ccctggctca
    36961 tcacaactac acgatcagct acttcggtcg cttcttcctg atcgtgggtc acaaaaacgc
    37021 tggtgaattt tagttcttca tggagttgac gcagccatcg acgcagctct ttacgcacct
    37081 gcgcatccag cgcgccaaac ggttcatcaa gcagcagaat ttgcggttct acagcaagcg
    37141 cacgcgccag cgctacacgc tgtttctggc cgccggaaag ctgcgccgga taacgatccg
    37201 ccagatgggc aagctggacc atttccagca attttgtcac tttcgctttg atggctgcgg
    37261 cattcgggcg ctcgcgacgc ggcagcaccg tcaggccaaa agcgatattg tcgaacaccg
    37321 tcatatggcg gaacagcgcg taatgctgga acacgaaacc gactttacga tcacgtgcgt
    37381 gcaggcggct cacgtcggtg ccgtggaagc gaatatgccc gctggtttga tgctccagcc
    37441 cagcgataat gcgcagcagc gtggttttcc cggaaccgga cggccccagc aacgcgacca
    37501 tctgacctga aggaatatcc agtgagatat cgttcagcac ctgggtgcga ccaaacgact
    37561 tcttaatatt ggcaatctca atgctcatga ttttcctcct gctgcgcgcg tttttcctga
    37621 ttctccaggc gccactgcaa catactcttt aaaaacaggg tgataatcgc catcagcgtc
    37681 aacagcgcgg cggcggtaaa ggagccgact gtgttgtagt cctgctccag caattcaatc
    37741 tgtaacggca gcgaaagtgt ttcgccacga atcgagccgg aaaccaccga caccgcgcca
    37801 aactcgccaa tcgcgcgggc gttggtcagc actacgccat aaagcagcgc ccagcggatg
    37861 ttcggcaatg tgacacggcg gaacatctgc cagccggatg cgccaagcaa aatcgccgct
    37921 tcgtcttcct gactgccctg gcttaacatc accggcacca gttcgcgcac cacaaacgga
    37981 cacgtcacga agatcgtgac cagcaccatt ccgggccagg agaacataat ttgcagatta
    38041 tgttcatcca gccagccgcc gagcgggccg ttggagccgt agaacagcag atacaccaga
    38101 ccggcaacca ccggtgacac ggcaaaagga atgtccagta gcgtcagcag taactggcgt
    38161 ccggggaagt taaagcgcgt caccagccag gccagcagaa taccgaacac caggtttacc
    38221 ggtacggcaa tcagcgcgat catcaccgtc agccagatgg cgtgcagcat atccggatcg
    38281 gccagattct gtaaaaccgg catcagccct ttgctgaacg cctgtacgaa gatataaatc
    38341 atcggcacca gcaggatgaa cgccgatacc agcatcccga tgccaatcag aaaccatttg
    38401 ccccagttaa tcgggcgcgc gtcataacgc ttcaattgtg tcacttccgc cattaatgac
    38461 ctaccacacg ccgaccaaag cgactttgca gagtgttaat tgagaacagc agcagcagag
    38521 atgccgcgag gatcaccgaa gcaatcgcgc tcgccgccgg gtaatcaaac tcctgtaagc
    38581 gaacgaaaat catcagcgac gtcacttccg tcttccaagc gatgttcccg gcgataaata
    38641 tcaccgcgcc aaactcacca agactacggg taaacgacaa cgccacgccc gccaccagcg
    38701 ccggagaaag ttccggcagc accactttgc agaaactctg ccagcgcgtt gcgccaagcg
    38761 tttctgccgc ttcttcatat tccgggccta actcttccag caccggctgc acggtacgta
    38821 ccacaaacgg aatgctggta aaggccattg ccaccgcaat ccccagccag gtataggtga
    38881 ctttgatatc aaacttcgcc agccattcac cgtaaaaacc gtttacagaa aagagcgagg
    38941 ccagcgttaa accggcgact gccgttggca gcgcaaaggg taaatccatc agcgcatcaa
    39001 gcagcgtgcg gcccggaaag cgataacggg ttaaaatcca cgccatcagc aggccgaaaa
    39061 cgccgttaaa aattgatgcc acaaacgccg acagcagcgt cactttgtag gccgcgacca
    39121 cctgcgggtt ggtgatcact tcccagtact gcgcccagct catctcagaa agctgcatca
    39181 ccagcgcgga gagtggcagc agcaatatca ggcacacaaa cagcagactg gtgccgaggc
    39241 ttaaggtaaa gcccggcagg acgcgtcttg atgaaactgc aaacattaat tacgccccgc
    39301 cgctaacagc ttgtctaact cgccgccgct ggtaaagtgg gttttcatca cttccggcca
    39361 ggagccaaat ttgtcttcca cgcggaacag ttcggtctgc gggaatttat ctttcagttt
    39421 gtccatgact tccgggttat tcacgcggta gtaatagtcg gtgatgatgg tttgcgcctg
    39481 cgggctgtaa agccagttca gataggcttt tgctgctttt tcggtaccgt tggcttgcac
    39541 gtttttatcg acccacgcca ccgggaattc tgccagaatg ttggttttcg gaatcaccac
    39601 ttcaaagccc tgcgcttcgt actgtttgcg gatgttgttc acttccgatt caaagctaat
    39661 cagtacatcg cccaggccgc gctcggcaaa ggtggtggtc gcgccacgac cgccggtatc
    39721 gaacacttca acgtttttca ggaactgagt cataaactgt tcggttttgg ctttgtcgcc
    39781 accgtcagct ttatccgctg cgccccatgc cgccagatag gtataacgcg cgttacccga
    39841 cgttttcggg ttcgggaaaa tcagcttcac gtcggagcgc accaggtcgt tccagtcgtg
    39901 gatattcttc gggttgccct tacggaccag gaagcccatg gtggagtaga acggcgagct
    39961 gttattcggc aggcgcgact gccagtccgc cgggataagc ttgcctttat cgtgcagaat
    40021 ttgtacgtcg gtcacctggt tataagtgac aacgtcggct tttaagccct gcaaaattgc
    40081 cagcgcctgt tttgatgacc cggcatgaga ttgttttatc gtcagtttgt cgccgccgtt
    40141 atctttggcc cattgctgtt caaacggcgg gttcagggcg gcaaacagct cgcgggagac
    40201 gtcataagaa ctgttcagca gttccgttgc ctgtacatgg cccgccagca gcagagaagc
    40261 gaccagcgcg agtgagttct ttttcagtaa gttaacggcc attgcgcacc cttataaatt
    40321 taatgacttt ctaatagcca tcatatttat aacggacgtg aaaggagtaa cggttttata
    40381 taccgtttgg tgatttggaa gttgaaaagg gaataagacg aaatcctccc cacaaatgca
    40441 gggaggaaac agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc
    40501 catcaatcac attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg
    40561 ccagttcgcc gacttccagc ggatcggcga ggcgacgcag cgggattgct tttgccattt
    40621 cagtcagcac cgactctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg
    40681 gtgtgcgaac gtatccaggg catatggcgt taacgcgaat accagactgc gcatactcca
    40741 ccgccagcga ttttgtcagg ccaacaatcg ccgctttcgt taaggcgtac gccgtttcac
    40801 ccggatcggc caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc
    40861 gggcaatcat ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatgt
    40921 caatatggaa atcgcgatcg tcatcgctca tatcgaggaa actgcccaga cgacaaacgc
    40981 ctgcgttatt caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg
    41041 cggctaccga cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac
    41101 cacacagttc gtccgccagc ttttcgatct caggggagat atccagcaag attaggtttg
    41161 cgccatgacg cgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa
    41221 tcagtgctgt cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg
    41281 ttacactcca ttccctctga aaatacagga taaacaaaat attactgcgg atcagctcac
    41341 taattcagga aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag
    41401 ctcactgctg cgctgaatca ttttcaatga ctccacatca ttgagttgca caagaccaac
    41461 aaacagtaaa tcggtaacag aattttgcgc cgtgcgggta gacatcgacg agctacgcca
    41521 ttcggtttca ccggaaacgg tatcgagcgt gaaatgcgcc agccgccgca gcggagagtc
    41581 agccagagaa gtaatggcaa tcaccgttgc cccctgcttt cgtgccgctt ctgcgcacaa
    41641 gacaatctct tttttactgc cgctgtaaga gatagcgatt tgtacatcgc cttttttcaa
    41701 cgcctgggaa acggtggctt gtacgtgcgt gtcggcttcg caggccacgc gatatccaat
    41761 tttcatcagt ttgaacgaca gatcgcaccc caccagcgcc gatccgccca ggcccgttat
    41821 ctggataaac ggtgctttac tgatgacctc aatgattttt tgcagccgcg cgtagtcgaa
    41881 caacgcacag gtttgctcca gcgccagctc tttttcgcga ttcaatttgc gggcaatcac
    41941 ttccagcgaa tcatcactgg taatcgaact gtgcaggtgc tgcgccgtgg cattcgtttt
    42001 ctcccggctg gcactgtatt cgcctattaa cgccatacgc aattcagtaa acccttgtgc
    42061 gccgagcttc tgggcaaact tcacaatact cgactggctg atgccgagct gtttcgccat
    42121 cttgcgcgaa gagacggatt tcagctcact cacgttggcc cgcagaaagt cagcaatttt
    42181 ttgctcgttc tcggtaaatt cactttccgc attgcgtatt ttcgtcaggt acaacatctt
    42241 ccactctcct gggctggcag caaaaattcc gcttaagttc ttatgacgct cttcacactc
    42301 tgcgagtgta gactcaatga ttcctttaag tctgtcaatt cgggatatat gattccatac
    42361 atctgcaatt atttgatcgt aaatagtaag gtcaccaccg atgcaacttg aaaagatgat
    42421 taccgaaggc tcgaacgccg cctcggctga aattgaccgc gtatcaacgc tggaaatgtg
    42481 ccggattatc aacgatgaag ataaaaccgt accgcttgcc gttgagcgcg tactgccgga
    42541 tatcgccgcg gcaatcgatg ttatccacgc ccaggtaagc ggcggcggtc gtctgattta
    42601 tctcggtgcg ggaacatccg gtcgtctggg gattctggat gccagcgaat gtccgcccac
    42661 ctacggcgtg aaaccgggtc tggtagttgg attaattgct ggcggcgaat atgccattca
    42721 gcacgcggtg gaaggcgctg aagatagccg ggaaggcggt gttaatgatt tgaaaaacat
    42781 cggattaacc gcgcaggatg tggtggttgg cattgctgcc agcggtcgca cgccgtatgt
    42841 gattgccgga ctggaatatg cacgccagct cggctgccgt acagtgggaa tttcctgtaa
    42901 tccggggagc gccgtttcca ccaccgctga gtttgctatt acgccggtgg ttggggctga
    42961 agtggtcacc ggttcttcgc ggatgaaagc aggcacggcg cagaaactgg tgctcaatat
    43021 gctttccacc gggctgatga ttaaatccgg caaagtgttc ggcaacctga tggtcgatgt
    43081 ggtcgccacc aacgaaaaac tgcatgtgcg ccaggtcaat atcgttaaaa acgccaccgg
    43141 atgtaatgcc gaacaagcag aagcagcgtt aattgcctgc gagcgcaact gtaaaacggc
    43201 cattgtgatg gtgctgaaaa atctcgatgc cgcagaagct aaaaaacgcc tggatcaaca
    43261 cggcggcttt attcgtcagg ttttagacaa ggaataaccc atggccaaag agatcagcag
    43321 tgaacttctg aacaccattc ttacccgtgt cggcggaccg ggaaatatcg ccagttgtgg
    43381 taactgtatg acgcgcctgc gtctgggtgt acatgacagt tcactggttg atcccgatat
    43441 caaaacgctg gaaggcgtga agggcgtcat tttgaccagt gaccaggtgc aggtcgtttt
    43501 tgggcctggt aaagcgcatc gtgccgcaaa agcgatgagc gagctgctgg gagaagcacc
    43561 ggtacaggat gccgcagaaa tcgccgccca gaacaaacgt cagttaaaag ccaaacaaac
    43621 ctccggcgtg caacaatttc tcgccaaatt cgccaccatc ttcacgccgc ttatccccgg
    43681 ttttattgcc gccggtctgc tgctggggat agcgacgtta attgccacgg tgatgcacgt
    43741 tccggcagat gctcagggca cgctacccga cgcgctgaat tttatgaagg tgttcagcaa
    43801 aggtttgttc accttcctgg tgattctggt gggctataac gccgcccagg catttggcgg
    43861 cacgggcgtc aatggcgcaa ttattgccgc actctttttg ctcggttata accccgctgc
    43921 aaccaccggt tactacgccg gttttcacga tttctttggt ctgcccatcg atccgcgcgg
    43981 caatattatc ggcgtgttga ttgccgcgtg ggcctgcgcc cgcatcgaag gcatggtgcg
    44041 ccgctttatg cccgacgatc tcgacatgct gctgacctcg ttaatcaccc tactgatcac
    44101 cgctacgctg gcctatctga tcattatgcc gctgggcggc tggttattcg aaggtatgtc
    44161 gtggctgttt atgcacctga acagtaatcc gctcggttgt gcggttttag ccgggctgtt
    44221 cctgatcgcc gtggtatttg gcgtgcatca gggctttatt cctgtttacc tcgcgttaat
    44281 ggacagccaa ggattcaaca gcttatttcc catcctttcg atggcaggcg cgggccaggt
    44341 gggcgcggcg ctggcactct actggcgggc gcaaccgcac agtgcattac gcagtcaggt
    44401 acgcggggcg attattcccg gcctgctggg cgttggcgaa ccgttgattt atggcgtcac
    44461 cctgccccgc atgaaaccgt ttattaccgc ctgtttaggc ggcgcggcgg gcggtttgtt
    44521 tatcggattg atagcctggt ggggtctgcc gatgggctta aacagcgcct ttgggccgtc
    44581 tggtctggtg gcgctgccgc tgatgacttc agcacaaggc attcttccgg cgatggcagt
    44641 ctacgctggc gggattctgg tggcatgggt ttgcgggttt attttcacca cgctttttgg
    44701 ctgccgtaac gtcaatctgg actgatatca tgaaacggac aatgctctat ctttctctgc
    44761 tggctgttag ttgtagcgtc agcgccgcaa aataccctgt tctgacagaa agttcgccag
    44821 agaaagcagg gttcaacgtc gaacggctta accagatgga tcgctggatt agccagcaag
    44881 tggacgccgg ttatcccggc gtaaacctgc tgattataaa agataatcag attgtgtatc
    44941 gcaaagcctg gggagcggcg aaaaagtacg atggcagcgt attgatggcg cagcctgtaa
    45001 aagctaccac cgggacgctg tatgacctgg cctcaaacac caaaatgtat gccactaact
    45061 tcgctctgca aaagctaatg agcgaaggca aactgcatcc tgacgatctg attgcgaaat
    45121 atatttcggg gtttgccgat agtccaaatg acaccatcaa aggcaaaaac acgctgcgga
    45181 tttctgacct gctgcatcac agcggcggtt tcccggcgga cccacaatat ccgaataaag
    45241 cggtcgcggg cgcgttatat tcccaggata aaggccagac gctggagatg atcaagcgca
    45301 cgccgctaga gtatcagccc ggcagcaagc atatttacag tgatgtcgat tatatgctgc
    45361 ttggatttat cgtcgagtcg gtcaccggtc agccgcttga ccgctatgtt gaagagtcga
    45421 tttatcgccc gctcggcctg acacatacgg tatttaaccc gctgctgaaa ggctttaaac
    45481 cgcaacaaat tgccgccacg gaattgaacg gcaatacccg cgatggagtg atccacttcc
    45541 cgaatatccg cacctccact cactggggtc aggtgcacga tgaaaaagcc ttttattcga
    45601 tgggcggcgt ttccgggcac gcaggtttgt tttccaatac cggcgatatt gcggtgttaa
    45661 tgcaaacgat gctgaacggc gggggctatg gcgatgtgca gctgttcagt gcggaaacgg
    45721 tgaagatgtt caccaccagc tctaaggaag atgccacttt tggcctcggc tggcgcgtga
    45781 atggtaatgc caccatgacg ccgacgtttg gcacgctggc aagcccgcaa acctacggtc
    45841 acactggctg gaccggaacg gtgaccgtta tcgatccggt gaatcatatg gcgattgtga
    45901 tgttaagcaa caagccacat tcgccggttg ccgatccgca aaagaatccc aatatgttcg
    45961 aaagcggtca gctgccgatt gcaacgtatg gttgggtagt tgatcaggtg tatgcggcgt
    46021 taaagcaaaa gtaataaaaa ggccagtcag taatgttact gcctggcctt taatcaatga
    46081 atcagaaacg cttacagcgc catcaacttg tccagcgacg gtgcgaaata atagccgccg
    46141 gttaccggtt tggtgaaacg caacatcgca tcacgcttac catcggtatc gccaaacatg
    46201 ctcagcagtt gctgctcaat gttatgcaga cgcgcgcagt aggcgcagaa gtacagaccg
    46261 tgagtgccac tggcggtgcc gtacggcagg ctctggcgaa caatcttcag ccctttgcca
    46321 tcttctttca gatcaacgcg ggtcaggtga gaggtttccg gacgttcgtc gccgtcaatc
    46381 tcttcgttgg cctctttggt gcgcccgatc atcatctcct gatcgtgaac gctcatccgg
    46441 ttgagctgct tcaggttgtg ctcccaacgc tgcacaaaca catagctgcc gccggcatcc
    46501 acgccgtctt tgataaccgc cacttcgcga cgcgtctctt cacccgccgg gttttctgtg
    46561 ccgtcaacaa agccgctcag gtcacgctct tccacccaac ggaagccgtg gatctcttct
    46621 ttcacttcaa tgcagtcacc aaaggcttcc atcgccgcct gggcgacaga gaagtttacg
    46681 tcgtgacgca gagaaagaat gtggatcaac acatcgaact gggtggtcgg cgcaaggcct
    46741 ttaccgtagc ccggaaaatc tttcagctct tctgccccaa cgccgccgct cagagcgcgc
    46801 cagatgttgt taccaaaggc aaccaccgca ccaagatgcg cgtccgggaa tttcgcttca
    46861 aaagttgcca gtttgtcggc aaatgtttta ctggccgcac gcagggcgtc aacttcccct
    46921 ttcacgttgg cttcgatcca aatcgccgcg cggcaatgtt ctggcaaaat gccactctga
    46981 acctgagaca ttgttcctcc tgaaaataat aatgccacgc aatccgtggc attgttgagc
    47041 gctattttac gtgttttttc gcggcggcgg tttgttcaga cgcaaattaa cgccgccaga
    47101 taattttact gactttccag tttttcaggg tatcgtccga aggcattaac ccttcaggac
    47161 cactccattc cccgctgaac tggtagctaa tatgttgact gccttcggct ttacattcga
    47221 cggcacgatt atcatcacca tcagcttttt ggcaattgcc aaatgctttg ctgtaaaggt
    47281 cgctaaacgg tgtaccgatt ttaacaccgg tgtcagcagg aatatcgcta tccagcacgt
    47341 caatgcgact gatcgtgcct tgatcgccgt taatcaccat cgccacgttg tcgcccttca
    47401 tcacttcaaa aaagcgcacc acgttgccgt tcgcggtttt cattccgctg cgcaggcgat
    47461 aatcgccatc aaccgcatcg gcaatggctt gttcttgcag tggtgtggac gccgttaatt
    47521 caccaacgcc ctgctcgctc actttggtgg acgacccaaa ccagttccac gggttagcgg
    47581 cagaccagtt aactgacgac atcgtggaac agccggtcag catcaatggc atagcgcata
    47641 acattaaacg cagcgatttc atgtcacttc ctttggttat tcaataacgt tgcttggagt
    47701 gcaaattcac caaaaagtgc cgttattctt ttacttcatt aaaacaagcg cgtaaacgtc
    47761 gattggtcag cagccagatt aacgccacaa tatccgccac taccagcgcc agaccgatac
    47821 cgctaacgga ttcaccgttc agccacagcc acggttgcca gcaaagtaaa accacctgcg
    47881 ccaacagcaa cagaaaatag agtacacgcc aggtgcgagg aaacgtagcc cgccgaccgc
    47941 tcaacaaaaa cgccagcacc gccggaatgc caggaatcag ccccagccag aaattatcgt
    48001 gatcgggata aaacagattt agcagcgcag taccctgctc gcgcgacgca ccggcaatga
    48061 caaacagcac ccaggttcgc gcctgaagca atagcacaag ccagaagagc aagggtaaac
    48121 gcaggcgacc gtgcgcatca taatggacag gatgaaactc agtactcttc atcttcaatc
    48181 aaacgcttac ccagactcag cacgtcggcg tgttcatatc ccaggcgttc atacattccg
    48241 agcaccatgt cgttatcttc cggcacgttg atctgaattt tcgggcagcc gcgagcaatc
    48301 agctttttct ccagccggtt aagcaacgca ttggcaatcc cacgcccacg aaactctgga
    48361 tgcacgccaa gataataagc agacccgcga tgcccgtcat aaccgcccat caccgttccg
    48421 accacttcac cgttcacctc agcgaccaga aacaaactga cgtcatggtt catcttacgc
    48481 tcgatgtcca tttccggatc gttccacgga cgcagcaagt cgcaacgctc ccaaagggtg
    48541 atgacctctt cgaaatcttc ctggcgaaat acgcgtatct ccatggtatt cgttaccttt
    48601 ttgcgggtta aaaggctgat tatggcgtga acggtcgaat tagccaatat ctgacgaaaa
    48661 tcggttgaaa aagtggcata atggggagtt gtcaactatt gaaatgaaaa gtaaaacaat
    48721 tctcaacagc aaacggtcgt aacggattac gcgatacgat ataacatctg gaactttatt
    48781 attacaactc aggccgtatg agcactttta aaccactaaa aacactcact tcgcgccgcc
    48841 aggtgctgaa agccggtttg gctgccctga cgttgtcagg aatgtcgcaa gccatcgcca
    48901 aagacgaacc tttaaaaacc agcaacggac acagcaagcc gaaagctaaa aaatctggcg
    48961 gcaaacgtgt cgttgttctc gatccaggcc acggcgggat tgataccgga gcgatcgggc
    49021 gcaacggttc gaaagaaaaa catgtggtgc tggcgattgc taaaaacgtc cgttccattt
    49081 tgcgtaatca tgggattgat gcgcgtttaa cgcgttctgg cgatacgttt atcccacttt
    49141 acgatcgcgt tgaaatcgcc cataaacatg gcgcagatct gtttatgtca attcatgccg
    49201 atggctttac caacccgaaa gctgccggtg cttcggtatt tgccctctct aaccgtgggg
    49261 caagtagcgc aatggcgaaa tacctgtctg aacgcgaaaa ccgcgccgat gaagttgccg
    49321 gtaaaaaggc gactgacaag gatcatctat tgcagcaagt gctgtttgat ctggtgcaaa
    49381 ccgatactat taaaaacagc ctgacgctcg gctcgcatat tctgaaaaag attaagccgg
    49441 tgcataaact gcacagccgc aacaccgaac aagccgcgtt tgtggtgttg aaatcgccgt
    49501 cggttccttc ggtgctggtg gaaacctcgt ttatcaccaa cccggaagaa gagcgactgt
    49561 taggcacggc ggcgtttcgt cagaaaatcg ccacagcgat tgctgaaggc gtgatcagtt
    49621 atttccactg gttcgacaac cagaaagcac attcgaaaaa gcgataagtt atgaaacccg
    49681 acgcacacca ggttaaacag tttctgctca accttcagga tacgatttgt cagcagctga
    49741 ccgccgtcga tggcgcagaa tttgtcgaag atagctggca gcgcgaaggt ggcggcggcg
    49801 ggcgtagtcg ggtgttgcgt aatggtggtg ttttcgaaca ggcaggcgtc aacttttctc
    49861 acgtccacgg cgaggcgatg cctgcttccg ccaccgctca tcgcccggaa cttgccgggc
    49921 gcagtttcga ggcgatgggc gtttctctgg tagtgcatcc gcataatccg tatgttccca
    49981 ccagccacgc gaatgtgcgc ttttttattg ccgaaaaacc gggtgcagag ccagtctggt
    50041 ggtttggcgg cggcttcgat ttaacccctt tctatggttt tgaagaagac gccattcact
    50101 ggcaccgcac cgcccgtgac ctgtgcctgc catttggtaa agacgtttat ccccgttaca
    50161 aaaagtggtg cgacgattac ttctacctca aacatcgcaa cgaacagcgc ggtattggcg
    50221 ggctgttctt tgatgatcta aacacgccag atttcgacca ctgttttgcc tttatgcagg
    50281 cggtaggcaa aggctacacc gacgcttatt taccaattgt agagcgacgt aaagcgatgg
    50341 cctacggcga gcgcgagcgc aattttcagc tctaccgtcg cggtcgttat gtcgagttca
    50401 atctggtctg ggatcgcggc actctgtttg gcctgcaaac tggcgggcgc accgagtcga
    50461 tcctgatgtc aatgccgcca ctggtacgct gggagtatga ttatcagcca aaagatggca
    50521 gcccagaagc ggcgttaagt gagtttatta aggtcaggga ttgggtgtaa ctccctcacc
    50581 cccattcccg catccgctga tgcaacgtca acgacggctt ctcggcaaac agctgctggt
    50641 aatccgtggc aaattgcccc agatgccaga atccccactg catggcggcg tcttttaccg
    50701 ttgtgctttg cgaccacggg cttatcagtt cgcggcgtac ggcgttcagg cgaatgcgtt
    50761 tcagccacgc gttcgggcca attcctaaaa tagcgtgaaa cgcgttttgt agcgtgcggc
    50821 ggctgacatg cagttgatta cacaagtcca gcaccgtcac cggttcggac atgttttcca
    50881 gcacatattc acgggcgcgg gaaagcaacc gacggtaact ctgatgactg atgctttccg
    50941 ccgtcaccat tggctgcgct tcttccagca tcgcccccat cgccattagc aaattatccc
    51001 ccagcacttt tcgcactgca ggctgatgga gattttccgg gttctcgcaa aacgtcgcca
    51061 gcgcctgttg gacaaagccc cacagcgcgg ctttatgctg ctctttcact tccagcgccg
    51121 actggctacg caacatatgt aatacccgat ccggattatg caaaaagtta gcctgacggg
    51181 tgatgacatc ttcagaaagc actacgccca ggatcgtgta atcgtccggc gtacttaatt
    51241 caaactcggt tccaccaggg cgcgtcgcta tttctgcgct tcccagacat tgcgaaccga
    51301 taaatccctg ctcaccgcgc gtcgccggaa tgccaaacca gaacgagttc ggccagacca
    51361 gacacgactg acgcagcgcc agaccggtgt attcacgaaa aacctgaata tcatcgagta
    51421 gaatttccgt aaattcacca tgaaacttgc ccggatgcag ctgatcgtaa atctgctgcc
    51481 aggcggtaat cgttaaagca tgttcataga catccgttgt ctgtcgttga tgaacattat
    51541 ccacttcgac cttcggcgtg agcttcaggt tttcgggtaa gggttcatga taaagatggt
    51601 gcaaattggc tgtacgggtc tttttcatga tgttaatgcc gggtgttgta ggacacccgg
    51661 cacctccgac aggttaatgg ggcttgagac gataacgact actgcgttta cgtaacgtcc
    51721 cagcagaaaa gagctgttcg agcgcatttc tggctttttc cagcggccag ccaaagtggg
    51781 cagccacttc tcctgccgtc actccctgac gtactgatgt taacagcgcc aacaactcgt
    51841 cagcagattc cgcgacgata actggcgcgt cgggttcctt tacgggttgc ttcggctggc
    51901 ggttaaagcc agtaaccagc cactgggtgt catcgtctgg ccgaccgatc tccttacggc
    51961 tgatgacacg cccggtacgc atcgcggcag cacaacctgc gtccagcgca gcacgacacg
    52021 ccgccagatc gccttccacc accagcgtta agcgacctgg gtcaagcact tcgtgactga
    52081 gcagacgaac gttagctgct ttgagcatgg catccgctgc atctatcgcg gcgaccatgc
    52141 cgtccacttc cagcaatccc agtgcattga tcattggcaa cctccgttac gcacgctgga
    52201 ttggattacg cgcgatttcc agcactgcat cggtaaaggc gttacaggcc gctttacacg
    52261 ctgcctggct accggttaaa aatgctgccg agtagttagt ttcagacggt ggcgggacat
    52321 aggtcaccag ctgtacgtcg gcagatttca acgctgcatc aatgccgtag gtcgcttcca
    52381 gcggcggtgc caccagatac gccatcggat cgccaagcgt gatcccggcg gttgatgaga
    52441 gataagaacc ggtacgcgaa actacatgtg ccaggaatgc cgtatcttgc gcatcattag
    52501 cccactggaa agcagcgcca ttttcaatat gcgcaaccat cgcatccaga ccggcacgca
    52561 cttccgccgg gtttggccca ccaagcataa tcagcacctc accggcagtc ggtgacgggc
    52621 cgtgtgccgc gcccgcatac agcgagcgac catataccac ttctaccatc gcttgtttgg
    52681 tcgcttcgtc agccgcaatg taggttacgt cgtcagaatc ggcagaaatc agaccgaggc
    52741 tacgaatatg tggcggcaat ttcagttcac gcgcaaaatc ggcgttaacg gaggcaatca
    52801 cccgcatggc ggttaccgac ggtcgaatca aatctaaagc tggcatgatg cctccttatc
    52861 gggtcatgtt gatgccggat gctttctgct ccagcatacg tttcgccaaa tccacaatga
    52921 cggctgcggc ttcaaccggc ggcgtgccgc cctggtgaat gttagagata caggtgcgat
    52981 cggcctcaac ggtggtcgcc atacgcggcg agtaaacggc gtagcaggag aggctttctg
    53041 actgacccaa ccccggacgt tcgcccacca gcaggatcac tactttcgcg ccgaggatct
    53101 caccgatctg atcttcaatc ttcacgcgac cataacgaac aaagaatggc gtgccgactt
    53161 tcagcccggc ctgtttcagg cccgccatca gcggcggcag gatctcttcg tagttcacag
    53221 tgatggcatc agttgacagg ccatcagaaa taacgacctg tacgtccgga ttagcaacac
    53281 actgtgcttt cagcgcttca acagcttctg cacacaggcg gcggcccata tccgggcgag
    53341 tcaggtagag atttttgtcg ctaatttcgg agcgtacttc cagcaagccc tgcgctttca
    53401 cccactcttc cggcacttct ttcagtacgg tatctttcga acgagagtga tcggccagga
    53461 aacgcagcag cgccagcgta cgcggacgcg gaccggcacg accggtacaa acgcgggcca
    53521 cggtgctgcg acgcagttct gttaacacat ccgcacgatg tgggttttca acaccaatcc
    53581 acacttttgc ttccgcagaa cctaaatcca gcgcgcagct ttccgaggtc accggtgccg
    53641 cacaggtggt agtggcgcac tttgcttctg acggcgcggg ggccgtttgt cccattgacg
    53701 ccatcacgct gcgtacaatt tcttcaatct gtttttgatc catgatgtgt tatctccgcg
    53761 tcatcagaag aacagtgacg gatcgcccgc ccgtttggtc aggcgaccgt ttgccataat
    53821 gcccatgctt tccagccagc gttcaaactc cggtgacggg cgcaggttga gtaactgacg
    53881 cacagtggcg gtatcgtgga atgcggtggt ctggtagttg agcatgatgt catcacccag
    53941 cggcatcccc atgatgtagt tgcagcctgc ggtggcgagc aggatcatca ggttttcgtt
    54001 gaggttctgg tcagcgtcag cgtggttggt gtaacagcag tcacagccca tagagatgcc
    54061 gctcagcttg cccataaagt gatcttctaa gcccgcacgg ataatctggc ggtcgttgta
    54121 gagatactcc ggcccaataa agccgaccac ggtgttgacg ataaacggat cgtaatgacg
    54181 cgccagccca tagttacgtg cttccatcgt cacctggtct gcgccgaagt tagcgccagc
    54241 ggatagcgct gaaccttgtc cggtttcgaa gtagaggcag ttttccccgg cgatacagtt
    54301 gaactctgca cccactgcgc gcgcttcgtc gagcatcgcc agttccacgc caaactcttt
    54361 cagccctttt tcgctgccac agatactctg gaaaatcagt ccgccaggcg cgccgcgacg
    54421 aatcgcttcg atctgggtgg tgacgtgcgc cagtacgcag ccctgagttg ggatgttgaa
    54481 tttgtcgatc acgccataga tggtgtccag cacgcggctt aagttttcca cgtcgtcagt
    54541 caccgggtta acgccgatca ccgcatcgcc caccccgaag gaaagccctt cgtagatttg
    54601 cgcggcgata ctttgcacgt cgtcacgggt gtcatttggc tgcaaacggg cgctgaaggt
    54661 gcccggaata ccgatggtgg tattggcctt tttgattacc ggcattttct tcgcgccgta
    54721 gatcaggtcc gcgttggagc aaatcttcgc taccgccgcg accacttccg aggtcagccc
    54781 tttgcgggta aaggcaatgt cgtccacgct ggtttcatcg ctcagcacat actcacgcag
    54841 ttcgctgatg ctccagtttt taatctggtt gtaggccgtt tcgttaacat cgtcctgaat
    54901 cagccgcgtc acgcagtcat cttcataggc aatcaccgga ttattgcgga tgtccgctac
    54961 ggtcatttcc gacaacacct gctttgccgc cacgcgctcc tgtgagcttg ccgctgcgac
    55021 gcccgccagc acatcccccg aacacagttc gttggcttta gccagcacct cttttacatc
    55081 cttaaactga tatacattgc cgaacaatgt ggtctttagt ttcataagtc gttccctcag
    55141 gaaggaaatg cgagtgattt caccgtcacc ggcacaaccg atccgccaaa aagaggcgta
    55201 ccaatgtcga tatagtcccc cgcgcggaca atcacttcgt caatgactgc caacgggagt
    55261 tgttgtagct gtgggcgcaa cagcatgccc agagctttac caaagtcctg cccggccacc
    55321 accagcaggg gatgcggatt cggaaaacgc gcgacgaaat cgaccaacgc gttgatgacc
    55381 gtcagtaccg cggcgtaacg cacaggcagc gaggcgggaa gcgccagcac gtacgcgtca
    55441 gttttgggat caagatccag ctgaatcagc gcctgttgcc aggcactcac cagatccgtt
    55501 tcatcaatcg ggatcgccac cggcaaattg cgcagcggca gttgtacgcc ctccagccag
    55561 attgtgctgc cagagagcga aagggtatgt gcgcccgcgc caatcaccgt ggcgcgcacg
    55621 gtttgcgccg gaaactgcac gttcatctca cgcaggcgcg gatggtcatg tagcgccgtc
    55681 gccagtagcg ggccaatatc ggcaaaacag aacgggtcag cgggctggtg gcgataacat
    55741 tcgcccacgc cgccagaaag cgtaatgatt tcgggcgtaa cacctgcggg cagcaaaccg
    55801 gtttgcatta atgcctgcgc gagcggcgaa agcgttccgt caatcacttc gacaatcagc
    55861 tctgccatcc gtcgggtaac ctgcaccagc tgcgcgccgg tcagcgaacg gacgtcagtg
    55921 cctgcaccga aacactcatc cacaatcatc tgccccggtt tatgagcgta aaccacgcgc
    55981 ccctggctgt cggtttccag caggcgacca ccgacgttga ggcaggcagt gccgctgatt
    56041 tttccggcat cgaacagggc gtagttcgcg gtgccaccgc cgatgtcgat attcagtacc
    56101 cgacacagcc gttgttcaga aagggtttgc gccccggcac cgtgaccggc gatcacggat
    56161 tcgaggtgcg gcccggcgct ggcgacgaca aaatcgccca gcgactgaga gagcgccatc
    56221 accgccgggc gagcattgcg ggttttcgcg ctttcaccgg tgatgatgat ggcaccagaa
    56281 tcaacgcttt ccggcgcaat gcccgcagcc tgatattgct ctaaaatcag cgatttcagt
    56341 tccgcttctt ttaaaccacc ctgtttatcg acaggggtaa agaacaccgg gctttgccag
    56401 ctaatttcgc gtttgatgaa ttcgtagcgc ggcacctgcg acaccgccgc acggttaacc
    56461 agttccaggt gggagaaaat cacctgggtg gtggtggtgc cgatatcgat accgacgctc
    56521 agtagctggc gagtgttcac gattgtgcct ccgcttcggt tttagccgcg gtcgcgtcgt
    56581 cttttggcac cagcatcatc gccacgccaa tcgccgttac gccgccgatc aacttgccga
    56641 caatcatcgg gaagatcatg gcattcatgt tggcagcggc gaagcctaag tggtcgccca
    56701 gggcgaaagc agcggaaacg gcgaaggcgc agttgatgac tttgccgcgg gtatccatct
    56761 gcttcatcat gccgaacatc gggatgttgt tggcaagcgt tgccaccatg ccggctgccg
    56821 cgatgttgtt catattcagc actttaccga cgctcatcag cggtttttca aaccagcggg
    56881 tcagcagcag caccatcgga tacgccccta acagaacgca ggagatagaa ccgataactt
    56941 caatggcgcg catcacctca ccgggtttat cgccaggggc cataaagata ggatccagac
    57001 cggggatcag ttcccagcca agcaggaatt tcactaccgc agcggcaaga ccgagggtga
    57061 tcaatgcaac gaggaatttg gcgaagatct ggaagccgtt gatcattttt tctgggatga
    57121 atttcagccc cagcgccacc agaatcgcaa caatgatcac cgggatcatg ttcatcagga
    57181 tcagggcgaa agtgaattcc accggctggc cgttgatctg aacaccggag tacatagcaa
    57241 ccagaccacc agcgatacaa ccaatcggaa tggtcacaat gcccgccagc acgccgagcg
    57301 ccagataacg acggtcagaa ggttcgataa tgccgagcgc caccggaatg gaaaacacaa
    57361 tcgttggccc catcatcgac ccgagaatta acccagagta tagccacgcg gctacgtcgc
    57421 cgcccgccag ctctttggcg aggaagaagc cgcccatatc gcacgccagc agtgttccgg
    57481 cgaacatcga tgggttagcg ccgagcattt cgtaaaccgg aataattacc ggcccgagta
    57541 cgtgagccag taccggtgcc agcgcggtca taccgaccat cgccaggccc agtgcgccca
    57601 ttgccataaa gccttcttcg aactgaccgc cggatccttc gatactttta ccgaacttac
    57661 cgaggaaacg agcagaaccg ccgaactgtg acaggatcct gtcaacggca gctatcagca
    57721 taaagaacat catgatgtac atgatgattt cgttaattcc catcgccttt actccctgtt
    57781 agttgttatt tattagcgga tgctgcgtga agccttatcc gcactacatg tgcaatccca
    57841 cagaccggat aagacgcggc atttattgcg ccgctgcgta caggccgaca agctgttcca
    57901 ggctggcggt acgcgggttg ctgcgcagac aaatatcttc cagcgcggcc tgcgcccatg
    57961 caccgtaatg cgcagatgtc gcgccaacat cgcccagtcg tttaccaatc ccaacttccg
    58021 caatcagctc acttaccgcg ttaatagcgt cacgatcgtc ggatttttta gttcgcagtg
    58081 cccgaccaat ctgactaaag cgttcgcgac aaaccatccg gttaaactcc atcaccgttg
    58141 gcagcaacat agcgttcgcg agaccgtgcg gaatatgcag cgccgcgccc ggctgatgcg
    58201 ccatcgcgtg gcacaaccca agacccgcac tggaaaacgc cattcccgcc atacatgaag
    58261 ccagcaacat gctctcgcgc gcggcaaggt cgtgaccgta gcccaccgct ttcggcagcg
    58321 atttgccaat catcgcaatg gcaccaatcg ccagactgtc ggtaaacggt gtagcgttca
    58381 gggcgctgta tgcttcaatg gcatgggtta acgcatcaat gccggtcatc gccgtgacat
    58441 gcgacggcac accttcggtc aatgcggcgt cgaggatcgc cacatccggc atcagcgagg
    58501 catgggctaa cacctgcttg cgcccgctca ccgcgtcgat aatcaccgtt acattggtgg
    58561 tttcagagcc ggttccggcg gtagttggaa tggcaatcag cggcaagcgc ggttgcagaa
    58621 cgctggtttc tgacatctct gccagcgtgc tatccgggtt cgtcaccagc aacgccacgg
    58681 ctttcgccgc atccagcacc gagccgccgc caaatgcgat caccccgtca caacctgact
    58741 cacgcaactg cgccacggct gcacacacgt cggtaatgca cggttcgccc accggacatg
    58801 gccagagcgt catggcgata cctttaacgg ccaggctacg cgtcagcccg gcggtcatcc
    58861 ctgcctgatg caaaaagctg tctgccatca cgaacagatg tttcagccca cgcgtttgcg
    58921 cttgctgtcc gcaactgctc accgagcccg gaccgcaaag cgtcaccggt ggaacgctaa
    58981 atgtttttac ccgttgcaga ttcagggtat cgaacgcctg aaagagcgcg gtctgcaatt
    59041 cattttgcat atagcccctc cgctttctct cgcccactac tggcgatcgc caacggggtc
    59101 ataaacaaac tgtgctgcgg taaatgcacc tgtaacgccg ggaattgttt gcgaaacagt
    59161 tctgccacgc ccggttgcat acaggagccg cccgccagcc ataaatcagt aatgccctgc
    59221 ccttcaatat gccgggcgac aatgtccgcc attttttcgt acaccggttt caccgtaggc
    59281 caaatctctt caccgtgacc gcgcttgtac tgctccgcct cttccagcga aatacggcga
    59341 tttccggcga aagtcagaga gatgtgatgt ccgccggtgg cttcatccgc cgagtacgtc
    59401 actttgccct ttttcacgat ggcaatgccg gtagtgccac cgccgatatc cactacaccg
    59461 gcgttgtcca gttgcagcag atccgcgact gcggttggct catccagcac atggctcact
    59521 tccagcccgg cagactccag cacgttgatg gaaatacgcg ggtccgtacc tggaggaaat
    59581 gaagtcgccg catggctaaa acgacgaccg aattgctgct cgagcgtgtc gagatggcga
    59641 cgaacaatgg tgaccgcgcc gaagaaatcc cagacgatgc cgtcgcgaac cacatcggcc
    59701 cagtcgaggc ataccgccac tggctgaccg tcgcggtcga caaccatcga caccacatcg
    59761 caggtgccca gatccactcc cagccacagc ggagattccg tcgcggcggg cgtctggtta
    59821 cacagcgttg ccgccgtttg cagacgtggg gtgagccatt gttcgtcgtg cgccatctgt
    59881 tactccttaa acaatgcgaa acgcatcgac taatacacag cgacgcagac ggacaaacgt
    59941 acgcgcgctg gtcacccctt caccggttgg cgtggtgatg gtcatggtgg tccagccttc
    60001 cccgcccagc cccagcccgg caatgcacgg tccgttctta acgaaaatgc tggtatcaac
    60061 agcattcgcc atctggttca tgttttcgat gttgcgcgag tgcattgccg ccgtgtggtg
    60121 gcaaccgcct tccagtttca ccgctagcgc aatggcatcc gccacgttgg cgacgcgcac
    60181 gacgggcaac accggcatca tcagttcagt cacggcaaac ggatgttctg cggtggtttc
    60241 cacaaacagc aggcgcgttt cttgcggaac tttaaggccg attgccgccg cgattttgcc
    60301 tgcgtcgcga ccaacccagt cacggctgac ggtgcctttt ccgcgctcgt cgatattttt
    60361 cagcaacacc ggttgcagct gctgcgcctg ttctgcggtc agtttcaccg cgtgctggcc
    60421 ttccatcaga cgcatcagtt catcggctac gctatcaaca acaatcagta ccttttcgtc
    60481 ggcacaaatg atgttgttat cgaaagacgc gcctttgacg atggactgag cagcacgggc
    60541 gaggtcagcg gtttcatcca ccactaccgg cgggttgcca gcgcctgcgg caatcagacg
    60601 tttattggtg tgtttacgcg ccgcttctac taccgcttcg ccgccggtca ccaccagcag
    60661 gccgataccc ggaaacttga acaagcgttg cgcggtttcg atatccggat ttgccacagt
    60721 aaccagtaag ttttccggcc cgcctgcggc aacaatcgcc tggttgagca gcgtgatcgc
    60781 ccgctgggag acttttttcg ccgccggatg cggggcaaaa atgacgctgt tgcccgcggc
    60841 aatcaggctg atggcgttat taattacggt tgccgccggg ttagtggacg gcgtcaccga
    60901 agccaccacg ccccagggcg cgttttcaat taaagtcagg ccgttgtcgc ccgtcagcac
    60961 ttgcggagag agacactcaa cgcctggcgt gccgcgtgcc tgagcgacgt ttttggcaaa
    61021 tttatcttca acgcgaccca tgccggtttc actgacggca agttccgcta aatctctggc
    61081 gtgtttttcg cctgcttcac gaatggcagc aatggctaac tggcgcattg ccacgctctt
    61141 taacccttgc tgggcaattt tggctgccgc aacagcgtca tccagggacg cgaaaacgcc
    61201 catctcatga acggcggcgg gcggcgtgtc actgctttgc attttcagca gtaccgcttt
    61261 caccacctgt tcaatatcct gttgattcat gatgttctgc cttatttgtg gaaaattacc
    61321 tgaccgccag acaccacctc atcgacgatg ccaatcacgc acagatcgac cggtgacgtt
    61381 tcgcttttat gcgcctggcg ggcggaactg ccactcacca gcaacaccca ctccccggtt
    61441 cccgcgccaa tattgtcgat ggcgacggca cattgcccgt caggattacc atgtggatca
    61501 atcatttcca ccatcagcaa tttgtcatgc gccagtccgt gatggcgtac ggtacaaaca
    61561 atttgtccag tgacgactgc cagtttcata cccgcctccg tggcgtattt caggtaaaag
    61621 ctccccctac cctccgcgga aggtaaaatg aaaaatgaga gagcgtgacg cccgaatcga
    61681 cgtcacacag ggtgattaca ggttgctgct atcgcctttc aggccgatcg ggaacacttc
    61741 ttccaggtcg ccgtgcggac gcgggataac gtgtacagat accagctcgc cgatacgctg
    61801 tgccgctgcg gcaccggcat cggtcgccgc tttacaggct gcaacatcac cacgaaccat
    61861 ggcagtacac aggccgccgc caatctgctt aacaccaacc agcttgacgc gcgcagcttt
    61921 caccatcgcg tcagaagcct caatcagtgc aaccaggccc cgggtttcga tcattcctaa
    61981 tgcttccatt gtgctttcct ctttatcagg gtccagaacg ggaccgttca ttcaaccagt
    62041 gtttgtaaac tgctttcgcg gttcacttct gtctgacgcg gcacagctgc caccagcgcc
    62101 agctcgataa tttcctgcac actacaacca cgagagagat cgtgcatcgg cgcggcaagt
    62161 ccttgtatca atggcccgac ggcacgatat ccgccgagtc gttgtgcgat tttgtagcca
    62221 atatttccgg cttccagcga cggaaaaacc atcacattgg ccttgccctg tagcgggctg
    62281 gcaggcgctt tttgcgccgc cacttccggc acgaaggcgg cgtcaaactg caactcgcca
    62341 tcgactacca gctttggtgc gcgctcacgg acgatttctg tcgcctgctg gacattggca
    62401 acgcaggggt gacgggcgct accgttactg gaaaacgaca gcatcgccac gcgcggctct
    62461 tctccggtga tggcgcgcca ggtttcggcg ctggcaagcg cgatatccgc cagctgcgcc
    62521 gccgtcggct gtggtaccac gctgcaatcg gcaaagccca acgccggacc gctgtactgt
    62581 ggcagcatca ggaaaatgga tgagagcgtt ttacagcctg gctgcaagcc gataatgcgt
    62641 aatccggcac gcagcacatt cgccgtggaa gagaggttgc ccgcgataca gacatccgct
    62701 ttaccggcgc tgaccattgc ggcggcgaac atcagcggat cggtaagttt ttccagcgca
    62761 tccggcggcg ttttttcgcc cgcgcgggcc agccagcgat gagcaaattc ttcccgcatt
    62821 gcgaggttgc catgcggatc tatcacctgt agcccgtcca tcgccacgcc gtgactgagc
    62881 gcaaactgac gaagttcaaa cggattggcg accagaatgg gcgttgccag accttgttga
    62941 tgtaaatatt gcgcagcttt cagcacacgt tgatctaacg catccggaaa aaccactctg
    63001 gcgggcgctc gcaacgccag ttcacaacaa cgttcaataa tcattgcgtt tcccccagtc
    63061 gttgttggat ctgcttcacc gttagcggct gcttcgttac gctcaggatc atcatcacgt
    63121 agaccgtgct ggaaaggcgg ttcagcgcct gcaagatgtc cgggcgtaat acttcaaaac
    63181 tgcgggtgat aaacacctgc gccgctacgg tttcggcttc ccgcactttg gtgcgtagca
    63241 gattcagcaa cgccgcatcg cgcccgtggc ttgcttccgg caccagatga tcgtgaccaa
    63301 gataacgcaa cggctgatga gaaagccggt gcagatcttc gtcagaaagc ccgacaatcg
    63361 cctggcagcc cagcggttcc gccagttcaa tttgcagcca cactgccagc gcaatggtgc
    63421 tgtcgagaac tgcgcgaaaa cccagacgcg gatcgctttt ggcgaccatt ttttccgccg
    63481 acaggtgagt cagcgtatcc ggcttttttg ccaccggctg gcggcacagt tcgcagcacg
    63541 cctgcggatg ttcatcgcta ctggttagcc catgaacggg ctgcggctgc tgctgttcat
    63601 cgtcaacaaa caggcggccc tgctcgtcaa taaacttgat gcgcagatgg cggctttcca
    63661 gcaactcccg ggcagagggc gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt
    63721 cgctgagcgt atggttcgct cttagccatg cttcggtgat gaaatctttc atagggattg
    63781 ccagttagcc ggccaggcga catacaggaa tttcacgctg gatgtcgtac caaattcgat
    63841 gctggagcct ttcgggataa acatcacgtc gcctgcttta gcgatcatgg tttcgccttc
    63901 gtggcgaaca tggagctccc cttccagcac catgtcgatt tcgtcgtagt tcagggtcca
    63961 cgggaagaat gcgttctccc actgcataaa accggcggcc atgctgctgc cgtcgtctcc
    64021 ggtcacaaga tcagtcaagc ccacgcaatg cggttgtgcg ccatcaaagc gaccaaactt
    64081 gacgctgcta ccatcaatca ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg
    64141 ctgcattgca ccctgctcca gcgactgttt ttccttcatc actttttcca tcagctgcgc
    64201 gaccaggctt tcggtaaact ggccttccgg tagctgggcg ataatggttt cgcggatgcg
    64261 ctggctttcg gttttatcgg ctggcacgct ggcgggcacg gacgccgtta ccgggatcaa
    64321 ttcatcacat tcggtgatgg taaaacccag tagatccgcc acttcacggg cttccggggt
    64381 aataatgctg gcgcgcagaa ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg
    64441 aatatcattc gctgtgatga gttttttcac ctgcttcctc ctgtttggtg agagatgcca
    64501 gataatccac cagttgctgc acgctttgcg ggtcgtggct gtttagctcg aaaatcggct
    64561 cctcgaagcc cgtctcaagc aataactttc gcgttgcggc gacgtcagca tccggcatgt
    64621 ccgttttact gatgacggcg atttgccgtt tgctgacgcc aatatccagc aacccggcag
    64681 gcaaccgact ttccgggtca ttcgcgccgt ggacatagat cagcatgtcc acatcctgta
    64741 gcgtggtaat taaggcgtga taccagcggg gatggctaaa atattcaccc ggcgtatcaa
    64801 tatcgccctt atcattaaat tccacggcct gtgtttttct ggcgagggta taattcccct
    64861 gcaacgcatt aaataacgtt gttttccctg ccccgacggt acccacaaac gcaattcgtt
    64921 tcatgaatgc ctcgtattaa cttttggtca tttcacaaag cgtataattt aataagcgac
    64981 ctaaaccact gacggtttgt gataacgctt cttctactgc gcccaccgag ccataaatca
    65041 ccaatgcacc actgaagcgg tcgagaaatc cgatatgcac atcggcggct ttcagcgcta
    65101 aatcaccggc gatcatcgcc gtttcgccgg gagttagcgt cataatgcca attgcgcccg
    65161 catccggaac gccgattttc ttcgccagtt cctcgccggg atgcgcgatg agatgcgcca
    65221 gtgtgacctg tttgccgggc acaaattcct gaatgatgcg ttctttatcc atgagtcgcc
    65281 gcctgtgaaa aactttcgtg tatcgtgaca atttttcgtc agagtaaata acaaaattcg
    65341 gcaagcgcgt ttaaaaggtg aggtagatca ctaagaatgg agagatattt actgttatga
    65401 ataaactttc atatgcgacg catgaatata aataaaataa tatcgagata ttttatatac
    65461 tgcttttttt attattcgca aactcagtaa taagctttac taatatattg cagctaaacc
    65521 gcttaccctg aatattcagg gtaagcgtga gagttaaaaa agaattacag cggttgggtt
    65581 tgcgcttcaa ccacggccag cgccaccatg ttgacgatac gacgcaccga tgcgatcggc
    65641 gttaacacgt gaaccggttt cgccacgccc atcagcaccg gaccgacagt cacaccttcc
    65701 gagctggaaa cgcgcagtaa gttgtaacta atgcgggcag cttccatgtt cggcatcacc
    65761 agaatattgg cagaaccttt caaagggctg tccggcatac ggtcgttgcg aatcgcttcc
    65821 accagcgctg catcgccgtg catttcacca tcaatcatca gttctggcgc acgttccctg
    65881 accagttcca gcgcctgacg cattttgctc gacgacgggc agtcagaaga accaaagttg
    65941 gagtgcgaca acagagcaac gcgcggttca ataccgaaac gacggacagt ttctgccgcc
    66001 atcaaggtga tctccgccag ctcttctgcg tctggttcgt cattgacgta ggtatcggca
    66061 ataaaggtgt taccactcgg cagcagcagc gcgttcattg cacctgcggt gtgaacgcca
    66121 tcgcgataac caaagacatt tttcaccacg ctaaaatgtt catgataatc acccaccgta
    66181 ccgcaaatca ttgcatcggc ttcgccacgc tgaaccatga tcgcgccgat cactgtcggg
    66241 ttactgatca gcgcccgctg cgcctgttcc tgagtgacgc cgcgacgctt catgatctgg
    66301 aagtattcgg tccagtactc tttaaagcgc ggatcggatt cgttattgac gatctcaaaa
    66361 tcaacgcccg ctttgatctg caagcccagt ttctgaatgc gcatttcgat cacgttcgga
    66421 cgaccgataa ggatcggttt cgccagtccc agcgttacca gttcctgagt ggcatgcaga
    66481 acgcgcgcct cttccccttc cggcagaact acgcgcttcg gcgctttgcg agcctgggag
    66541 aaaatcggct tcataaacag gttggttttg taaacgaact cagtcagctt gtcgatgtag
    66601 acgtcgaaat cagcaatcgg acgcgtcgcc acacccgact ccatcgccgc tttcgcgacc
    66661 gcaggagcaa tcttgactat cagacgcgga tcaaacggtt ttggaatgat gtattcaggg
    66721 ccaaagctca gatcctgatc gccatacgct gaagccacca cttcactctg ttccgcatgg
    66781 gccagttctg caatcgcgcg taccgccgcc agtttcatct cttcgttgat ggcggttgcg
    66841 ccaacgtcca gcgcgccacg gaagatgaac gggaagcaaa ggacgttgtt cacctggttc
    66901 gggtagtcgg aacggccggt acagatgatg gcatccggac gcacttcttt cgccagcggc
    66961 ggcagaattt ccggttccgg gttcgccagc gccaggatca ttggcgctcg agccattttc
    67021 ttcaccattt cctgggtcag cactttcggg ccggaacagc cgaggaaaat atctgcgcct
    67081 tcaatcacat catcgagggt acgtttgccg tcatccacta ccgcatatgc ggctttggtt
    67141 tccgccatgt ttggctcacg gccctgatag ataacgcctt ttgaatcgca aaccacgatg
    67201 ttatgttttt gcagacccaa cgctaccagc aggttcatac aggcgattgc tgcagcaccc
    67261 gcgccggaaa ccaccatacg cacgtcagag atgtttttct ccaccacgcg caagccgttg
    67321 aggatggcgg cagtgctgat aattgccgtg ccgtgctgat cgtcgtggaa taccggaata
    67381 ttcatccgct cgcgcagttt ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg
    67441 aggttgatgc cgccgaaggt cggttcgagc gcagcgacga cttcaataaa tttgtccggg
    67501 tcgagttcat caacttcaat gtcaaataca tcaatcccag cgaatttctt aaacagaacg
    67561 cccttgcctt ccatcaccgg tttgcctgcc agcgcgccaa tgttgcctaa ccccagcacc
    67621 gccgtaccgt tagagatcac cgccaccagg ttaccgcgcg cggtatattt gtaggctttt
    67681 aacgggtctt tttcgatttc aagacaaggt gcggcaacgc ctggtgagta ggccagcgcc
    67741 agatcgcgct gcgttgccag aggcttggtt ggagaaacct ggattttccc tggaactgga
    67801 aattcatgga aatcaagtgc actttgtttt aactggtcat ccatttggtt gttcctttca
    67861 cgtaacgttc acaaataaag tgtgtgggca acagcccctg cccacaacgt ggcgcacatt
    67921 attaccttgc cggagtctac agactttgag caagtccaaa ctctcaccat taatataatg
    67981 ttttggtaat aatcctataa cactgatgtt acctgcttaa tccagcaata ccatgcctgt
    68041 ctgctatgct tttttgatgc gtttagcgaa atttctcaga agtgtgaatt aacgcactca
    68101 tctaacactt tacttttcaa ggagtatttc ctatgaacga gttagacggc atcaaacagt
    68161 tcaccactgt cgtggcagac agcggcgata ttgagtccat tcgccattat catccccagg
    68221 atgccaccac caatccttcg ctgttactca aggctgccgg attatcacaa tatgagcatt
    68281 taatagacga tgctatcgcc tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg
    68341 tcgcagcgtg tgacaaactg gcggtcaatt tcggtgctga aatcctcaaa atcgtacccg
    68401 gtcgcgtgtc aacagaagtt gatgcacgcc tctcttttga taaagaaaag agtattgaga
    68461 aggcgcgcca tctggtggac ttgtatcagc aacaaggcgt tgagaaatca cgcattctga
    68521 tcaaactggc ttcgacctgg gaaggaattc gcgcggcaga agagctggaa aaagaaggta
    68581 ttaactgcaa cctgacgctg ctgttttctt ttgcacaggc gcgggcctgt gcagaagcgg
    68641 gcgtttttct gatttcgccg tttgtcgggc gtatttatga ctggtatcag gcacgcaagc
    68701 cgatggaccc atatgtggtg gaagaagatc cgggcgttaa atcggtgcgc aatatctacg
    68761 actactataa gcaacaccac tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg
    68821 aacaaatcct cgccttaacc ggctgcgatc gactgactat cgcaccgaat ttgctgaagg
    68881 agctgcagga aaaagtttcg ccagtggtac gtaaattaat cccaccttct cagacgttcc
    68941 cacgcccagc tcccatgagc gaagcggagt tccgttggga gcacaatcag gatgcgatgg
    69001 cggtagaaaa actgtctgaa ggcattcgtc tgttcgccgt tgatcaacgc aaactggaag
    69061 atcttcttgc cgccaaacta taaaccagcc acggagtgtt atatgtcccg aaaagacctt
    69121 gccaatgcga ttcgcgcact cagtatggat gcggtacaaa aagccaattc tggtcatccc
    69181 ggcgcgccga tgggcatggc tgatattgcc gaagtgctgt ggaacgattt tcttaaacat
    69241 aaccctaccg acccaacctg gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg
    69301 tcgatgctgc tctacagttt gctgcatctg accggttacg acctgccgct ggaagagctg
    69361 aagaactttc gtcaactgca ttcgaaaacc cctggccacc cggaaatcgg ctatacgccc
    69421 ggagttgaaa ccaccaccgg tcctcttgga caaggtttgg cgaacgccgt cgggctggcg
    69481 atagcggaac gtacactggc ggcgcagttt aaccagccgg atcatgaaat tgtcgatcac
    69541 ttcacctatg tgtttatggg cgacggctgc ctgatggaag gtatttccca cgaagtctgt
    69601 tcgctggcgg gcacgctggg actgggcaag ctgattggtt tttacgatca caacggtatt
    69661 tcgattgatg gagaaaccga aggctggttt accgacgata cggcaaaacg ttttgaagcc
    69721 tatcactggc atgtgatcca tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagca
    69781 atccttgaag cgcaaagcgt gaaagataag ccgtcgctga ttatctgccg tacggtgatt
    69841 ggctttggtt cgccgaataa agcaggtaag gaagaggcgc acggcgcacc gctgggggaa
    69901 gaagaagtgg cgctggtacg gcaaaaactg ggctggcacc atccgccatt tgagatccct
    69961 aaagatattt atcacgcttg ggatgcccgc gaaaaaggcg aaaaagcgca gcagagctgg
    70021 aatgagaagt ttgccgccta taaaaaggct catccgcaac tggcagaaga gtttacccgt
    70081 cggatgagcg gtggtttacc gaaggactgg gagaaaacga ctcagaaata tatcaatgag
    70141 ttgcaggcga atccggcgaa aatcgctacc cgtaaggctt cgcaaaatac gcttaacgct
    70201 tacgggccga tgctaccgga gctgctcggc ggttcggcgg atctggctcc cagcaacctg
    70261 accatctgga aaggttctgt ttcgctgaag gaagatccgg cgggcaacta cattcactac
    70321 ggggtgcgtg aatttggcat gaccgctatc gccaacggca ttgcgcacca cggcggcttt
    70381 gtgccgtata ccgcaacgtt cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg
    70441 gcggcactga tgaaagcgcg gcagattatg gtttataccc acgactcaat tggtctgggc
    70501 gaagatggtc cgacgcacca ggctgttgag caactggcca gcctgcgctt aacgccaaat
    70561 ttcagcacct ggcgaccgtg cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt
    70621 gagcgccaca acggaccgac ggcactgatt ctctccaggc agaatctggc ccaggtggaa
    70681 cgtacgccgg atcaggttaa agagattgct cgtggcggtt atgtgctgaa agacagcggc
    70741 ggtaagccag atattatttt gattgccacc ggttcagaga tggaaatcac cctgcaagcg
    70801 gcggagaaat tagcgggaga aggtcgcaat gttcgcgtgg tttccctgcc ctcgaccgat
    70861 attttcgacg cccaggatga ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct
    70921 cgcgtggcgg tggaagcagg tattgccgat tactggtaca agtatgttgg tctgaaaggg
    70981 gcaattgtcg ggatgacggg ttatggggaa tctgctccgg cggataagct gttcccgttc
    71041 tttggcttta ccgccgagaa tattgtggca aaagcgcata aggtgctggg agtaaaaggt
    71101 gcctgatggt gatcgccgga tgctgattgc cggatgcgac gctgacgcgt cttatccggc
    71161 ctacagcgtc ttatccggcc tacatgtccc gccattttgt ttaacgggtg atccacaacg
    71221 tgggccaggc gtctggccca tgccagttat cgcaggtggg ttctgcggcg taacgcacca
    71281 ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg cttaatccgc
    71341 gccctttcgc taaggtcacc agttcacgcg atttctcatc aaatgttgcg ttcatcagtt
    71401 ccagttcatt cgtctcctga ccgttgttga acggcaaacg cagccggacc gggcgcgagg
    71461 ccagcggctt tttacgcgac acaatccatg ccaaatcaat ggtgttatag gcccctgcct
    71521 cacagctaat catcatcagc gctttatcat cagtcagcgc agtgacattc acctcacgac
    71581 gcaatggatc aagcgagcag cgcagaccat tcatccgcca gtttccataa tccagcaaat
    71641 cgttgcgttc ttcgagtgag agtggcgtcg gcgttgggtt aaccaccacg acctctttca
    71701 gcgcaggcgc gggcggtacg ctgagcggcg gttcgtcccc tttcttgatc cacgcggttt
    71761 cactgccaac gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt aagtcactca
    71821 gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga atcatctgca
    71881 aaaacgcggt gatggttgcc gtatcatcgg ttactaataa ccatggtgaa atccgccact
    71941 tgtcaccact taacgccaac ggctcgccat ctaacagcag ccgtggcgca atttcccctt
    72001 cagacgcatc cggcgacttc aacccgccgc gctcaatacg taaaacggca tcggtatgcg
    72061 ccccggcgct gcggctcagg gtcatcacca gtccattatg atcgcccgta ttacgcgcca
    72121 cgcagaagtt ttggttatta caggtgacct gccagtcgga aaacgcccgt tgcgctggtg
    72181 ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa atgcgatagc
    72241 gcatggacgg tacgacccca gaagttcaaa caaacagcac aagtcgtatc ttcgttgcgc
    72301 aggtagcgcg gctcaatcgg atttgtcgga ttgagccaag aataaacaac gaaatattat
    72361 tgttattttt cagtctatta aatgtgacat ttgcaaatag ttaagcaata acaccgtctt
    72421 accgtcacgt atctcgccgg ttttgatcat ctccagcgcc tggctgaacg gcagctcgag
    72481 cacttcaata tcttcatctt cgacaccgcc accggcgtta gcgcgctgat tgtcactgta
    72541 ttcggcgata aaaaagtgga ttagctcagt cacaccgcct ggcgacatat acagttcaaa
    72601 taatttgcgc acttcgccca cttcatagcc cgtctcttca atcgcttctt tgcgaatgca
    72661 cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct gcccgctttc
    72721 attgccatta acccaggtag cgacacggaa ctgacgaatc agaaccacgg tctttttctt
    72781 cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg taaacttcac gtttatggcg
    72841 gataacttcg ccatctttgc gcgtgagatc gtaagtaatg ttgtgcaggg tgaaatagtt
    72901 atcggagaga attttgtctt taatgagggt gatttgttgc gtcataccga ctccacagcg
    72961 cgaaatgaac aattatatta cgctgtgaag tcgggtttgt ctgcgcaggc tatcagtgag
    73021 atttgactga ttttaccccc agccagtcaa taatcccctg tgccgcatga cgtccttctg
    73081 ccattgcggt aaccaccaga tccgcaccac gcacggcgtc gccaccggcg aagattttcg
    73141 gattggtggt ctggtaacgg tactggcttt ccacatccgc gatgatgcgg ccccatttgt
    73201 ctaccgttac accgtgcgac tccagccacg gcatcccgtg cggattgaag ccaaacgcca
    73261 taatcaccgc gtcggctggc atgacaaatt cactgccttc caccggcact ggacgccgac
    73321 gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa acgaatcccg cagacgtgac
    73381 cttgttcatt cagctcaagc gccaccggct ggacgttaaa ttcgaagttg gccccctctt
    73441 cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt agcttcatca cgacgataag
    73501 cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg cacacagtcc atcgcggtgt
    73561 cgccgccgcc cagtaccacg acgttaagtc cggcggtatt gataaacggc tcttccggta
    73621 gctcttcgag ccccatcacc tgtttagtgt tggcgatgag gaacggcagc gcgtcataaa
    73681 cgcccggcgc atcttcattg ggtaaacccg ctttcatgga acggtaagtg cctacgccaa
    73741 cgaagaccgc gtcgtattgt tccaaaagcg aatccaaaga gacatcttta cccacttcac
    73801 aattgagttc gaagtgaatc cccatcgcgc tgaagatttc ccgacgacgt gccagcaggg
    73861 atttatccag tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt tctggatggc
    73921 gatcgtacac cgtcaccgcc acgccattgc ggatcagaac atccgcacag gccagccctg
    73981 ccggacctgc accgataatc gccacccgct tgtccacttt ggtgacatgg cttaagtcag
    74041 gacgccaacc tttcgccaac gcctgatctg aaatgtagcg ttcaatgttg ccgatagtta
    74101 ccgcaccgtg ctcatcgcga atagtacagg caccttcaca caaacggtct tgcggacaaa
    74161 cgcgtccggt aatttccggc agggtgttgg tcaggtgaga aagctcgacg gcggcgtcga
    74221 tgtttccggc tttcaccagt tcaatccact gcggtatatg gttatgcagc gggcaggtcc
    74281 attcacaaac gctgtgctcg ccgcatttaa ggcagcgcga ggcttcccgt tgtgcctgat
    74341 cggcacggaa tggcagataa atttcatcaa aaccggtttt gcgcgcttca atcgccagtt
    74401 tatccggctc gccacgcgcg ggcgttgcct gcatttgttc gactttactc attaccggca
    74461 tttcttgtgc agcggtactg gcatgccacg gttgatgttc ctgacgtgcg gtacgcaagc
    74521 ggcgggattt cgccatgcct gagagtgcgg cgtcagtgac cagctgcagc gcgtccgccg
    74581 ggcaattctc gacacaggca ggaccgtttt cgcgccctgc acaaaggtcg catttatgcg
    74641 ccgtggcttt aactttgccc gctgcgacgg gtgtcaggac gatttgcatc gtaccaaacg
    74701 gacaggccac cacgcaggat ttacagccaa tacacttttg ctgattgacc tgaatgctgt
    74761 catcaacgtg gctgattgcg ccattagggc agctacgggc gcagggcgca tcttcacaat
    74821 ggtgacaggt cactgcacta cgttgctgct gatgtttgat aaccgtaatt cggggatgaa
    74881 aatggtgttg gctcaggaca tgttgctcat cattgtgagc catgacacaa gcgatttcac
    74941 aagcatgaca acccagacat tgctgactgt tggccataat aaaacgattc ataacgacct
    75001 tcttttttgg ttgtaaaaac cttattcttt atatgagtgt tgttattacc cgacttacag
    75061 gggaatcggc aatgttcatg tgcccagaat aagtaactat ttcgctataa actgtggcag
    75121 atcaaataat cccatcacta actaaattgc gtttcagcga actggaacat taatgatttt
    75181 ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc ggccagtctg gcccgggcct
    75241 ttttttacat tgtgctgctg tcgattcttt ccacgggtat cgctctgcta actctggcga
    75301 gcagtttgcg cgacgctgag gctatcaata ttgccggatc gctgcgtatg cagagttacc
    75361 gcctgggcta cgacttgcaa agtggcactc cacaactcaa tgcacatcgc cagctgtttc
    75421 agcaggcact gcattcaccg gtattaacca acctcaacgt ctggtatgtg ccagaagcag
    75481 taaaaactcg ctatgcgcat ctgaatgcca actggctgga gatgaataat cggctcagca
    75541 agggcgattt gccgtggtat caggccaata ttaataatta tgttaatcag atagacctgt
    75601 tcgtgctggc tttacagcac tacgctgaac gcaaaatgct gctggtggtg gcgatttccc
    75661 tggctggcgg tatcggtatt ttcacgctgg tcttttttac tttgggccgc atacgccatc
    75721 aggtggttgc cccgctgaat cagctggtta ccgccagtca gcgtattgaa cacggacagt
    75781 tcgactcgcc accgctggat accagcctgc cgaatgagct tggtctactt gcaaaaacct
    75841 ttaaccagat gtcgagcgag ctgcataaat tgtaccgttc gctggaggcg tcagtagaag
    75901 aaaagacccg cgatctccac gaggccaagc gtcgtctgga ggtgttgtat caatgttcgc
    75961 aggcgctgaa caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc
    76021 gcgacaatga agcggctgaa tatctggagt taaatgtcgg tgaaaactgg cggattagcg
    76081 aagggcaacc aaacccggaa ttgccgatgc agattttacc ggtgacaatg caagagacgg
    76141 tttacggcga actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca
    76201 gcgtttcgtc gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc
    76261 agttattgtt gatggaagaa cgtgcaacca tcgcccgcga attgcacgac tcgctggctc
    76321 aggtactttc ttacttacgt atccagttga cgttacttaa gcgttcgata ccggaagata
    76381 acgccaccgc acaaagtatc atggccgatt tttcccaggc gttgaatgat gcttatcggc
    76441 agttacgcga gctgttgact acttttcgcc tgacgctgca gcaggcggat ctcccctccg
    76501 cgttgaggga aatgctggat acgttacaaa atcaaaccag cgccaaactg accctcgact
    76561 gccgtctgcc aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc
    76621 gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg
    76681 tcaccgcgcc ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg
    76741 aaccgaaaga acccgaaggt cattatggtc tgaatatcat gcgcgaacgc gcggaacggc
    76801 taggtgggac gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct
    76861 ttcgctctgc ggagggtgag gaaagtcagt tgatgtaatg cctcctactg accaaagaat
    76921 acttgcagtt aaggttcagt ataaaagggc atgataattt acattaactc ctttttttct
    76981 ccacgattgg ctcgtacctt gccgctacag tgaagcaagt caagcctaca acgatacgca
    77041 gaaacacgag gtcctctttt aatggcgaat ttctttattg atcgccccat ttttgcctgg
    77101 gtgctggcaa tcctgttgtg tctgacaggt accctggcga ttttttcatt gcccgttgaa
    77161 caataccccg atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg
    77221 gcccagacgc tggaaaacac cgtgacccag gttatcgagc aaaatatgac cggcctcgat
    77281 aatctcatgt atatgtcatc tcagagcagt ggcaccggtc aggcatctgt cactttaagt
    77341 tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca
    77401 gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg tgactgtgcg taaaaccggc
    77461 gataccaaca ttctgaccat tgccttcgtc tctaccgatg gttcgatgga taaacaggat
    77521 attgctgatt atgttgccag taatattcag gacccgttaa gccgcgtaaa tggcgtcggg
    77581 gatatcgatg cctatggttc gcaatattcc atgcgtatct ggctggaccc ggcgaaactc
    77641 aacagtttcc agatgacagc taaagatgtc actgatgcca ttgagtcaca gaacgcgcag
    77701 attgcggttg ggcaacttgg tggtacacct tccgtcgata agcaggcgct caacgccacc
    77761 attaacgccc agtcactgct gcaaacacca gaacagttcc gcgatatcac cttgcgggtg
    77821 aatcaggacg gctcagaagt aaggctcggc gatgtcgcca ccgccgaaat gggggcggag
    77881 aaatacgatt atcttagccg cttcaatggt aagccagcct ccgggttggg ggtaaaactg
    77941 gcctccggcg cgaacgaaat ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg
    78001 gcgcagtatt tcccgcacgg actggaatac aaggtggcgt atgaaaccac ctcgtttgtt
    78061 aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc
    78121 gttatgtatc tgttcctgca aaacttccgc gccacgctga tacccactat cgccgtgccg
    78181 gtggtgttga tgggaacctt ctccgtactt tacgccttcg gttacagcgt caacacctta
    78241 accatgttcg cgatggtgct ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg
    78301 gaaaacgtcg aacgtattat gagtgaggaa ggactcactc ctcgcgaagc cacgcgtaaa
    78361 tcgatggggc agatccaggg ggcactggtc gggattgcga tggtgctttc ggcggtattt
    78421 gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct atcgccagtt ctctattacc
    78481 attgttgcgg cgatggtgct gtcagtactg gtagcgatga tcctcactcc ggctctgtgt
    78541 gccacactac ttaagccact gaaaaaaggt gagcatcacg ggcaaaaagg cttttttgcc
    78601 tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt
    78661 ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg catggtgttc
    78721 ctgttcctgc gtttgccgac gtcgttctta ccgctggaag accgtggcat gtttactacc
    78781 tcggtacaat tgcccagcgg ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc
    78841 gagaaatatt acttcaccca tgaaaaagac aacatcatgt cggtgtttgc cactgttggt
    78901 tctggccctg ggggtaacgg gcaaaacgtg gcgcgaatgt ttatccgcct gaaagactgg
    78961 agcgaacgcg acagtaagac cggcacctcg tttgccatta tcgagcgtgc aacaaaggcg
    79021 tttaaccaaa ttaaagaagc tcgcgttatc gccagcagcc cgccagcaat tagcggtctt
    79081 ggtagttctg caggttttga tatggagttg caggaccacg ctggagcagg tcacgatgcg
    79141 ctgatggcag cacgtaacca gttgctggcg ctggcggcgg aaaacccgga gctaacccgt
    79201 gtgcgccata acggcctcga tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa
    79261 gctcaggcgc tgggcgttgc tatcgacgat attaacgaca cactgcaaac cgcctggggt
    79321 tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca
    79381 gctgcgccgt atcgcatgct gccagatgac atcaatctct ggtatgtccg aaataaagat
    79441 ggcggcatgg tgcccttctc tgctttcgcg acctcacgct gggagacagg ctcgccgcgt
    79501 ctggaacgct ataacggtta ttctgcggtt gaaattgttg gggaagccgc accgggggtc
    79561 agtaccggta cggcgatgga tattatggaa tcgttagtga agcagctacc aaacggcttt
    79621 ggtctggagt ggacggcgat gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg
    79681 ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg ctgcattgta tgaaagctgg
    79741 tcggtaccgt tctcggtaat gctggtcgtg ccgctggggg taatcggcgc gctgctggca
    79801 acctggatgc gcgggctgga aaacgacgtt tacttccagg tgggcctgtt aacggtcatt
    79861 ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa
    79921 ggccacgacc tgtttgaagc gacgctccac gcctgccgtc agcgtttacg cccgattctg
    79981 atgacctcgc tggcatttat cttcggcgta ttgccaatgg caaccagcac gggtgccggt
    80041 tccggtggtc agcatgcggt gggtactggc gtaatgggcg ggatgatttc agccactatt
    80101 ctggctattt acttcgtgcc gctgttcttt gtgctggtgc gccgccgctt cccgctgaag
    80161 ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt
    80221 ccgattaaga acctgctcag cgggttcttg ctgtttgtac tttgtctcag gaattactta
    80281 cgaagcataa cttcaataaa gtctttccag ttccccagtt cacgttcaat cataacaacc
    80341 tctcttataa ttatgggtat tctacggaaa caatataccg tggtgaagct aatttactcg
    80401 attgctgcga tgactacctc cgggggacaa accttatgta aatactatgg tcctgcagtg
    80461 atgatttgta tgtgatacac agcaacattt cgagatattc atacggcatc taatactgat
    80521 ttaa
//
LOCUS       DABSYT020000013        75251 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.013, whole genome shotgun sequence.
ACCESSION   DABSYT020000013 DABSYT020000000
VERSION     DABSYT020000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 75251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.013"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            207..359
                     /gene="mokC"
                     /locus_tag="ID725_RS05900"
                     /old_locus_tag="pgaptmp_001180"
     CDS             207..359
                     /gene="mokC"
                     /locus_tag="ID725_RS05900"
                     /old_locus_tag="pgaptmp_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGV7849821.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(463..1593)
                     /gene="dnaJ"
                     /locus_tag="ID725_RS05905"
                     /old_locus_tag="pgaptmp_001181"
     CDS             complement(463..1593)
                     /gene="dnaJ"
                     /locus_tag="ID725_RS05905"
                     /old_locus_tag="pgaptmp_001181"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGV7849822.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     TKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1682..3598)
                     /gene="dnaK"
                     /locus_tag="ID725_RS05910"
                     /old_locus_tag="pgaptmp_001182"
     CDS             complement(1682..3598)
                     /gene="dnaK"
                     /locus_tag="ID725_RS05910"
                     /old_locus_tag="pgaptmp_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGV7849823.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3975..4379
                     /gene="yaaI"
                     /locus_tag="ID725_RS05915"
                     /old_locus_tag="pgaptmp_001183"
     CDS             3975..4379
                     /gene="yaaI"
                     /locus_tag="ID725_RS05915"
                     /old_locus_tag="pgaptmp_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGV7849824.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4405..5118
                     /gene="msyB"
                     /locus_tag="ID725_RS05920"
                     /old_locus_tag="pgaptmp_001184"
     CDS             4405..5118
                     /gene="msyB"
                     /locus_tag="ID725_RS05920"
                     /old_locus_tag="pgaptmp_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGV7849825.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5267..5833
                     /gene="satP"
                     /locus_tag="ID725_RS05925"
                     /old_locus_tag="pgaptmp_001185"
     CDS             5267..5833
                     /gene="satP"
                     /locus_tag="ID725_RS05925"
                     /old_locus_tag="pgaptmp_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGV7849826.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5868..6455)
                     /gene="mog"
                     /locus_tag="ID725_RS05930"
                     /old_locus_tag="pgaptmp_001186"
     CDS             complement(5868..6455)
                     /gene="mog"
                     /locus_tag="ID725_RS05930"
                     /old_locus_tag="pgaptmp_001186"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGV7849827.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6570..7523)
                     /gene="tal"
                     /locus_tag="ID725_RS05935"
                     /old_locus_tag="pgaptmp_001187"
     CDS             complement(6570..7523)
                     /gene="tal"
                     /locus_tag="ID725_RS05935"
                     /old_locus_tag="pgaptmp_001187"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGV7849828.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7802..9232
                     /gene="yaaJ"
                     /locus_tag="ID725_RS05940"
                     /old_locus_tag="pgaptmp_001188"
     CDS             7802..9232
                     /gene="yaaJ"
                     /locus_tag="ID725_RS05940"
                     /old_locus_tag="pgaptmp_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGV7849829.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVYTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            9302..10078
                     /gene="yaaA"
                     /locus_tag="ID725_RS05945"
                     /old_locus_tag="pgaptmp_001189"
     CDS             9302..10078
                     /gene="yaaA"
                     /locus_tag="ID725_RS05945"
                     /old_locus_tag="pgaptmp_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGV7849830.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENTRGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10231..10527)
                     /gene="yaaX"
                     /locus_tag="ID725_RS05950"
                     /old_locus_tag="pgaptmp_001190"
     CDS             complement(10231..10527)
                     /gene="yaaX"
                     /locus_tag="ID725_RS05950"
                     /old_locus_tag="pgaptmp_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGV7849831.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10741..12027)
                     /gene="thrC"
                     /locus_tag="ID725_RS05955"
                     /old_locus_tag="pgaptmp_001191"
     CDS             complement(10741..12027)
                     /gene="thrC"
                     /locus_tag="ID725_RS05955"
                     /old_locus_tag="pgaptmp_001191"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGV7849832.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12028..12960)
                     /gene="thrB"
                     /locus_tag="ID725_RS05960"
                     /old_locus_tag="pgaptmp_001192"
     CDS             complement(12028..12960)
                     /gene="thrB"
                     /locus_tag="ID725_RS05960"
                     /old_locus_tag="pgaptmp_001192"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGV7849833.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12962..15424)
                     /gene="thrA"
                     /locus_tag="ID725_RS05965"
                     /old_locus_tag="pgaptmp_001193"
     CDS             complement(12962..15424)
                     /gene="thrA"
                     /locus_tag="ID725_RS05965"
                     /old_locus_tag="pgaptmp_001193"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGV7849834.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15450..15563)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15505..15588)
                     /locus_tag="ID725_RS05970"
                     /old_locus_tag="pgaptmp_001194"
     CDS             complement(15505..15588)
                     /locus_tag="ID725_RS05970"
                     /old_locus_tag="pgaptmp_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGV7849835.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(15802..16488)
                     /gene="yjtD"
                     /locus_tag="ID725_RS05975"
                     /old_locus_tag="pgaptmp_001195"
     CDS             complement(15802..16488)
                     /gene="yjtD"
                     /locus_tag="ID725_RS05975"
                     /old_locus_tag="pgaptmp_001195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGV7849836.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16888..17028)
                     /gene="yjjY"
                     /locus_tag="ID725_RS05980"
                     /old_locus_tag="pgaptmp_001196"
     CDS             complement(16888..17028)
                     /gene="yjjY"
                     /locus_tag="ID725_RS05980"
                     /old_locus_tag="pgaptmp_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGV7849837.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17124..17840
                     /gene="arcA"
                     /locus_tag="ID725_RS05985"
                     /old_locus_tag="pgaptmp_001197"
     CDS             17124..17840
                     /gene="arcA"
                     /locus_tag="ID725_RS05985"
                     /old_locus_tag="pgaptmp_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGV7849838.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17900..19252)
                     /gene="creD"
                     /locus_tag="ID725_RS05990"
                     /old_locus_tag="pgaptmp_001198"
     CDS             complement(17900..19252)
                     /gene="creD"
                     /locus_tag="ID725_RS05990"
                     /old_locus_tag="pgaptmp_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGV7849839.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19310..20734)
                     /gene="creC"
                     /locus_tag="ID725_RS05995"
                     /old_locus_tag="pgaptmp_001199"
     CDS             complement(19310..20734)
                     /gene="creC"
                     /locus_tag="ID725_RS05995"
                     /old_locus_tag="pgaptmp_001199"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGV7849840.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20734..21423)
                     /gene="creB"
                     /locus_tag="ID725_RS06000"
                     /old_locus_tag="pgaptmp_001200"
     CDS             complement(20734..21423)
                     /gene="creB"
                     /locus_tag="ID725_RS06000"
                     /old_locus_tag="pgaptmp_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGV7849841.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(21436..21909)
                     /gene="creA"
                     /locus_tag="ID725_RS06005"
                     /old_locus_tag="pgaptmp_001201"
     CDS             complement(21436..21909)
                     /gene="creA"
                     /locus_tag="ID725_RS06005"
                     /old_locus_tag="pgaptmp_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGV7849842.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22120..22989
                     /gene="robA"
                     /locus_tag="ID725_RS06010"
                     /old_locus_tag="pgaptmp_001202"
     CDS             22120..22989
                     /gene="robA"
                     /locus_tag="ID725_RS06010"
                     /old_locus_tag="pgaptmp_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGV7849843.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22986..23633)
                     /gene="gpmB"
                     /locus_tag="ID725_RS06015"
                     /old_locus_tag="pgaptmp_001203"
     CDS             complement(22986..23633)
                     /gene="gpmB"
                     /locus_tag="ID725_RS06015"
                     /old_locus_tag="pgaptmp_001203"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGV7849844.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23685..24197
                     /gene="yjjX"
                     /locus_tag="ID725_RS06020"
                     /old_locus_tag="pgaptmp_001204"
     CDS             23685..24197
                     /gene="yjjX"
                     /locus_tag="ID725_RS06020"
                     /old_locus_tag="pgaptmp_001204"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGV7849845.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(24344..24670)
                     /gene="trpR"
                     /locus_tag="ID725_RS06025"
                     /old_locus_tag="pgaptmp_001205"
     CDS             complement(24344..24670)
                     /gene="trpR"
                     /locus_tag="ID725_RS06025"
                     /old_locus_tag="pgaptmp_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGV7849846.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24760..26697)
                     /gene="sltY"
                     /locus_tag="ID725_RS06030"
                     /old_locus_tag="pgaptmp_001206"
     CDS             complement(24760..26697)
                     /gene="sltY"
                     /locus_tag="ID725_RS06030"
                     /old_locus_tag="pgaptmp_001206"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGV7849847.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26908..28575
                     /gene="ettA"
                     /locus_tag="ID725_RS06035"
                     /old_locus_tag="pgaptmp_001207"
     CDS             26908..28575
                     /gene="ettA"
                     /locus_tag="ID725_RS06035"
                     /old_locus_tag="pgaptmp_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGV7849848.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28682..28849)
                     /gene="nadS"
                     /locus_tag="ID725_RS06040"
                     /old_locus_tag="pgaptmp_001208"
     CDS             complement(28682..28849)
                     /gene="nadS"
                     /locus_tag="ID725_RS06040"
                     /old_locus_tag="pgaptmp_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGV7849849.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(28883..30115)
                     /gene="nadR"
                     /locus_tag="ID725_RS06045"
                     /old_locus_tag="pgaptmp_001209"
     CDS             complement(28883..30115)
                     /gene="nadR"
                     /locus_tag="ID725_RS06045"
                     /old_locus_tag="pgaptmp_001209"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGV7849850.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30136..31518)
                     /gene="radA"
                     /locus_tag="ID725_RS06050"
                     /old_locus_tag="pgaptmp_001210"
     CDS             complement(30136..31518)
                     /gene="radA"
                     /locus_tag="ID725_RS06050"
                     /old_locus_tag="pgaptmp_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGV7849851.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31567..32535)
                     /gene="serB"
                     /locus_tag="ID725_RS06055"
                     /old_locus_tag="pgaptmp_001211"
     CDS             complement(31567..32535)
                     /gene="serB"
                     /locus_tag="ID725_RS06055"
                     /old_locus_tag="pgaptmp_001211"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGV7849852.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32641..33282
                     /gene="ytjB"
                     /locus_tag="ID725_RS06060"
                     /old_locus_tag="pgaptmp_001212"
                     /pseudo
     CDS             32641..33282
                     /gene="ytjB"
                     /locus_tag="ID725_RS06060"
                     /old_locus_tag="pgaptmp_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            33313..34329
                     /gene="lplA"
                     /locus_tag="ID725_RS06065"
                     /old_locus_tag="pgaptmp_001213"
     CDS             33313..34329
                     /gene="lplA"
                     /locus_tag="ID725_RS06065"
                     /old_locus_tag="pgaptmp_001213"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGV7849853.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34361..34624
                     /locus_tag="ID725_RS06070"
                     /old_locus_tag="pgaptmp_001214"
     CDS             34361..34624
                     /locus_tag="ID725_RS06070"
                     /old_locus_tag="pgaptmp_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7849854.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(34785..35504)
                     /gene="deoD"
                     /locus_tag="ID725_RS06075"
                     /old_locus_tag="pgaptmp_001215"
     CDS             complement(34785..35504)
                     /gene="deoD"
                     /locus_tag="ID725_RS06075"
                     /old_locus_tag="pgaptmp_001215"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGV7849855.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35561..36784)
                     /gene="deoB"
                     /locus_tag="ID725_RS06080"
                     /old_locus_tag="pgaptmp_001216"
     CDS             complement(35561..36784)
                     /gene="deoB"
                     /locus_tag="ID725_RS06080"
                     /old_locus_tag="pgaptmp_001216"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGV7849856.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36836..38158)
                     /gene="deoA"
                     /locus_tag="ID725_RS06085"
                     /old_locus_tag="pgaptmp_001217"
     CDS             complement(36836..38158)
                     /gene="deoA"
                     /locus_tag="ID725_RS06085"
                     /old_locus_tag="pgaptmp_001217"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGV7849857.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38285..39064)
                     /gene="deoC"
                     /locus_tag="ID725_RS06090"
                     /old_locus_tag="pgaptmp_001218"
     CDS             complement(38285..39064)
                     /gene="deoC"
                     /locus_tag="ID725_RS06090"
                     /old_locus_tag="pgaptmp_001218"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGV7849858.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39322..40871
                     /gene="yjjI"
                     /locus_tag="ID725_RS06095"
                     /old_locus_tag="pgaptmp_001219"
                     /pseudo
     CDS             39322..40871
                     /gene="yjjI"
                     /locus_tag="ID725_RS06095"
                     /old_locus_tag="pgaptmp_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            40843..41706
                     /gene="yjjW"
                     /locus_tag="ID725_RS06100"
                     /old_locus_tag="pgaptmp_001220"
     CDS             40843..41706
                     /gene="yjjW"
                     /locus_tag="ID725_RS06100"
                     /old_locus_tag="pgaptmp_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGV7849859.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQYPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(41920..42702)
                     /gene="yjjV"
                     /locus_tag="ID725_RS06105"
                     /old_locus_tag="pgaptmp_001221"
     CDS             complement(41920..42702)
                     /gene="yjjV"
                     /locus_tag="ID725_RS06105"
                     /old_locus_tag="pgaptmp_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGV7849860.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(42699..43772)
                     /gene="yjjU"
                     /locus_tag="ID725_RS06110"
                     /old_locus_tag="pgaptmp_001222"
     CDS             complement(42699..43772)
                     /gene="yjjU"
                     /locus_tag="ID725_RS06110"
                     /old_locus_tag="pgaptmp_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGV7849861.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(43894..44055)
                     /gene="ytjA"
                     /locus_tag="ID725_RS06115"
                     /old_locus_tag="pgaptmp_001223"
     CDS             complement(43894..44055)
                     /gene="ytjA"
                     /locus_tag="ID725_RS06115"
                     /old_locus_tag="pgaptmp_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGV7849862.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44182..44787)
                     /gene="osmY"
                     /locus_tag="ID725_RS06120"
                     /old_locus_tag="pgaptmp_001224"
     CDS             complement(44182..44787)
                     /gene="osmY"
                     /locus_tag="ID725_RS06120"
                     /old_locus_tag="pgaptmp_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGV7849863.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45180..46769)
                     /gene="prfC"
                     /locus_tag="ID725_RS06125"
                     /old_locus_tag="pgaptmp_001225"
     CDS             complement(45180..46769)
                     /gene="prfC"
                     /locus_tag="ID725_RS06125"
                     /old_locus_tag="pgaptmp_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGV7849864.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(46860..47537)
                     /gene="yjjG"
                     /locus_tag="ID725_RS06130"
                     /old_locus_tag="pgaptmp_001226"
     CDS             complement(46860..47537)
                     /gene="yjjG"
                     /locus_tag="ID725_RS06130"
                     /old_locus_tag="pgaptmp_001226"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGV7849865.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47552..47998)
                     /gene="rimI"
                     /locus_tag="ID725_RS06135"
                     /old_locus_tag="pgaptmp_001227"
     CDS             complement(47552..47998)
                     /gene="rimI"
                     /locus_tag="ID725_RS06135"
                     /old_locus_tag="pgaptmp_001227"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGV7849866.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(47967..48380)
                     /gene="holD"
                     /locus_tag="ID725_RS06140"
                     /old_locus_tag="pgaptmp_001228"
     CDS             complement(47967..48380)
                     /gene="holD"
                     /locus_tag="ID725_RS06140"
                     /old_locus_tag="pgaptmp_001228"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGV7849867.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48483..49514
                     /gene="rsmC"
                     /locus_tag="ID725_RS06145"
                     /old_locus_tag="pgaptmp_001229"
     CDS             48483..49514
                     /gene="rsmC"
                     /locus_tag="ID725_RS06145"
                     /old_locus_tag="pgaptmp_001229"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGV7849868.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            49704..49790
                     /locus_tag="ID725_RS06150"
                     /old_locus_tag="pgaptmp_001230"
     tRNA            49704..49790
                     /locus_tag="ID725_RS06150"
                     /old_locus_tag="pgaptmp_001230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49738..49740,aa:Leu,seq:cag)
     gene            complement(49829..50065)
                     /gene="yjjZ"
                     /locus_tag="ID725_RS06155"
                     /old_locus_tag="pgaptmp_001231"
     CDS             complement(49829..50065)
                     /gene="yjjZ"
                     /locus_tag="ID725_RS06155"
                     /old_locus_tag="pgaptmp_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGV7849869.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            50206..50994
                     /gene="fhuF"
                     /locus_tag="ID725_RS06160"
                     /old_locus_tag="pgaptmp_001232"
     CDS             50206..50994
                     /gene="fhuF"
                     /locus_tag="ID725_RS06160"
                     /old_locus_tag="pgaptmp_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGV7849870.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(51032..51709)
                     /gene="bglJ"
                     /locus_tag="ID725_RS06165"
                     /old_locus_tag="pgaptmp_001233"
     CDS             complement(51032..51709)
                     /gene="bglJ"
                     /locus_tag="ID725_RS06165"
                     /old_locus_tag="pgaptmp_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGV7849871.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(51667..52392)
                     /gene="yjjQ"
                     /locus_tag="ID725_RS06170"
                     /old_locus_tag="pgaptmp_001234"
     CDS             complement(51667..52392)
                     /gene="yjjQ"
                     /locus_tag="ID725_RS06170"
                     /old_locus_tag="pgaptmp_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7849872.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            53013..53783
                     /gene="yjjP"
                     /locus_tag="ID725_RS06175"
                     /old_locus_tag="pgaptmp_001235"
     CDS             53013..53783
                     /gene="yjjP"
                     /locus_tag="ID725_RS06175"
                     /old_locus_tag="pgaptmp_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGV7849873.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            53774..54247
                     /gene="yjjB"
                     /locus_tag="ID725_RS06180"
                     /old_locus_tag="pgaptmp_001236"
     CDS             53774..54247
                     /gene="yjjB"
                     /locus_tag="ID725_RS06180"
                     /old_locus_tag="pgaptmp_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGV7849874.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLGGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            54354..54893
                     /gene="dnaT"
                     /locus_tag="ID725_RS06185"
                     /old_locus_tag="pgaptmp_001237"
     CDS             54354..54893
                     /gene="dnaT"
                     /locus_tag="ID725_RS06185"
                     /old_locus_tag="pgaptmp_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGV7849875.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            54896..55633
                     /gene="dnaC"
                     /locus_tag="ID725_RS06190"
                     /old_locus_tag="pgaptmp_001238"
     CDS             54896..55633
                     /gene="dnaC"
                     /locus_tag="ID725_RS06190"
                     /old_locus_tag="pgaptmp_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGV7849876.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            55682..56176
                     /gene="yjjA"
                     /locus_tag="ID725_RS06195"
                     /old_locus_tag="pgaptmp_001239"
     CDS             55682..56176
                     /gene="yjjA"
                     /locus_tag="ID725_RS06195"
                     /old_locus_tag="pgaptmp_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGV7849877.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            56430..58721
                     /gene="opgB"
                     /locus_tag="ID725_RS06200"
                     /old_locus_tag="pgaptmp_001240"
     CDS             56430..58721
                     /gene="opgB"
                     /locus_tag="ID725_RS06200"
                     /old_locus_tag="pgaptmp_001240"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGV7849878.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(58858..59880)
                     /gene="lgoD"
                     /locus_tag="ID725_RS06205"
                     /old_locus_tag="pgaptmp_001241"
     CDS             complement(58858..59880)
                     /gene="lgoD"
                     /locus_tag="ID725_RS06205"
                     /old_locus_tag="pgaptmp_001241"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HGV7849879.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            60019..60933
                     /gene="lgoR"
                     /locus_tag="ID725_RS06210"
                     /old_locus_tag="pgaptmp_001242"
     CDS             60019..60933
                     /gene="lgoR"
                     /locus_tag="ID725_RS06210"
                     /old_locus_tag="pgaptmp_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGV7849880.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            61148..62509
                     /gene="lgoT"
                     /locus_tag="ID725_RS06215"
                     /old_locus_tag="pgaptmp_001243"
     CDS             61148..62509
                     /gene="lgoT"
                     /locus_tag="ID725_RS06215"
                     /old_locus_tag="pgaptmp_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7849881.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(62558..64222)
                     /gene="tsr"
                     /locus_tag="ID725_RS06220"
                     /old_locus_tag="pgaptmp_001244"
     CDS             complement(62558..64222)
                     /gene="tsr"
                     /locus_tag="ID725_RS06220"
                     /old_locus_tag="pgaptmp_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGV7849882.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            64451..64993
                     /locus_tag="ID725_RS06225"
                     /old_locus_tag="pgaptmp_001245"
     CDS             64451..64993
                     /locus_tag="ID725_RS06225"
                     /old_locus_tag="pgaptmp_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849883.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLLKEINNTPYCYIVI"
     gene            65286..67436
                     /gene="btsT"
                     /locus_tag="ID725_RS06230"
                     /old_locus_tag="pgaptmp_001246"
     CDS             65286..67436
                     /gene="btsT"
                     /locus_tag="ID725_RS06230"
                     /old_locus_tag="pgaptmp_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGV7849884.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            67486..67689
                     /gene="yjiX"
                     /locus_tag="ID725_RS06235"
                     /old_locus_tag="pgaptmp_001247"
     CDS             67486..67689
                     /gene="yjiX"
                     /locus_tag="ID725_RS06235"
                     /old_locus_tag="pgaptmp_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGV7849885.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            67700..68656
                     /gene="yjiA"
                     /locus_tag="ID725_RS06240"
                     /old_locus_tag="pgaptmp_001248"
     CDS             67700..68656
                     /gene="yjiA"
                     /locus_tag="ID725_RS06240"
                     /old_locus_tag="pgaptmp_001248"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGV7849886.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(68725..70083)
                     /locus_tag="ID725_RS06245"
                     /old_locus_tag="pgaptmp_001249"
     CDS             complement(68725..70083)
                     /locus_tag="ID725_RS06245"
                     /old_locus_tag="pgaptmp_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HGV7849887.1"
                     /translation="MQKRVLPIDVYSDETDEPRLIVRKKEHDLYKYYILQDSKDYKPE
                     KPSDTELVFISNAETIRDYLLRLSVDELKLLAKYILQNVYIVFVQTDDFASSFRLFNV
                     LNSRGLPLSNADLLKNALFESASTHNKKSEQIESAWSQIEDMVGVRRLDKFLTLHKLS
                     EKKDRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFEHPSIRRK
                     IASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMHGWLKKQIKSQR
                     EMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPRPNQVNLIKAILLR
                     LDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGNLTLISGS
                     KNSEAQHYDFIKKKSIYEKLNSKSSFDLTKDVCNSSEWGLAELKMRHEKMKTQLKKLW
                     LV"
     gene            complement(70092..70376)
                     /locus_tag="ID725_RS06250"
                     /old_locus_tag="pgaptmp_001250"
     CDS             complement(70092..70376)
                     /locus_tag="ID725_RS06250"
                     /old_locus_tag="pgaptmp_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HGV7849888.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENGYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKSSRFRG"
     gene            70654..73086
                     /gene="hsdR"
                     /locus_tag="ID725_RS06255"
                     /old_locus_tag="pgaptmp_001251"
     CDS             70654..73086
                     /gene="hsdR"
                     /locus_tag="ID725_RS06255"
                     /old_locus_tag="pgaptmp_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGV7849889.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            73153..74622
                     /locus_tag="ID725_RS06260"
                     /old_locus_tag="pgaptmp_001252"
     CDS             73153..74622
                     /locus_tag="ID725_RS06260"
                     /old_locus_tag="pgaptmp_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGV7849890.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            74622..>75251
                     /locus_tag="ID725_RS06265"
                     /old_locus_tag="pgaptmp_001253"
     CDS             74622..>75251
                     /locus_tag="ID725_RS06265"
                     /old_locus_tag="pgaptmp_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGV7849891.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYY"
BASE COUNT        18276 a        19929 c        18549 g        18497 t
ORIGIN      
        1 ttccggatta gaatcgataa aacagaatgg gttaaagaga gcgatataat agcggcgggt
       61 gcttgaggct gtctgtctca ggcattagct gaacggcaga tagagaaaag ccccgagtga
      121 tattttacca tcaacccgag gccccctata tgctgaacac atgtagagtg cctcttactg
      181 accgtaaggt caaggagaag agagcaatga agcagcataa ggtgatgatt gtcgccctga
      241 tcgtcatctg tatcaccgcc gtagtggcgg cgctggtaac gagaaaagac ctctgtgagg
      301 ttcacatccg aactggccag acggaggttg ctgttttcac ggcttacgaa tccgagtaag
      361 agcaacggcg gggagtgatc cccgccactt tacaggtgct cgcatatctt caacgcaccc
      421 tatttttacc caggcctgcc cacgggcagg cttttgggga ggttagcggg tcaggtcgtc
      481 aaaaaacttc ttcacaccat caaagaagct ctttgagcgc gggctgttgt gctcgccggt
      541 tgggccaccg aagctttctt gcagctcttg cagcagctgt ttctgcttct cgttcaggcc
      601 taccggtgtt tcgacgacaa cgcggcacag caaatcaccc tgtgcgccac cgcggacaga
      661 cttgacgcct ttaccgcgca tacggaatag cttaccggtc tgggtttcgc caggcacttt
      721 cagtttgacg cgaccatcaa gggtcggtac ttcgatttca ccacccagcg ccgccatagc
      781 gaagttgatc gggacttcgc aatacaggtt gttgccttca cgctcgaaaa tcgggtgctg
      841 tttaacctga acctgaacgt acagatcgcc tgccggtgcg ccgtgttcac ccgcttcacc
      901 ttcgcccgca agacggatgc ggtctccagt gtccacccct gccgggattt taacggacag
      961 cgttttgctg cgctcaacac gaccatgacc atgacatttg ttgcacggat ctttgatcag
     1021 cgtaccgcgg ccctgacagt gtggacaggt ctgctgcacg gcaaagaaac cctggcgcat
     1081 ctgcacctga ccagaaccat gacaggtcgg acaggtctgc ggctgtgtac ctggttttgc
     1141 accgctaccg tggcaaacgt cacactcttc cagagtcgga atgcggatct ctttggtcac
     1201 gccacgtaca gcttcttcga gggtgagctc catgttatag cgtaaatcag caccgcgcgc
     1261 cgcacgttga cgaccacgtc cgccgccaaa aatatcgccg aaaacgtcac caaaaatatc
     1321 gctgaagtct gcgccgccgc caaaaccgcc gccgcccatg ccaccttgct caaacgcagc
     1381 atgaccatac tgatcgtatg ccgcacgttt ttgcgagtcg gtcagaactt cataagcttc
     1441 cttgatctct ttaaatttcg tctcggcctc tttgtcaccc tggttacggt ccgggtggta
     1501 tttcatggcc aggcgtttgt aggccttttt gatttcacgc tcttccgctg ttttggaaac
     1561 gcctaaaatc tcgtaataat cttgcttagc catctttttt aaattgcccc tagatgaatg
     1621 cacgggcgga gaggaaattc cccttcgccc gtgtcagtat aataacccgt ttatagggcg
     1681 attatttttt gtctttgact tcttcaaatt cagcgtcgac aacatcgtca tctttcgcgt
     1741 tgtttgcaga agcatcagca ccggcagtct gctgctgggc atgttgctgc tgggcgattt
     1801 ccatcagttt ctgggaaacc tgtgccagtt cctgcatttt cgcttcgata gcggctttgt
     1861 cttcaccttt cagagcagtt tccagtgcag tcagcgcaga ctcgatagca gttttgtcgt
     1921 cagccggcag tttgtcgcct gcttcttcaa cctgcttacg ggtgctgtgc agcagatggt
     1981 cgccctggtt gcgagtctgt accagctctt caaacttacg gtcagcttcg gcgttagctt
     2041 cggcgtcgcg taccattttc tggatttcat cttcgttcag accagaagac gccttgatgg
     2101 tgatcttctg ctctttaccg ctgtttttat ctttcgcgga aacgtgcagg ataccgtcag
     2161 catcgatatc gaaggtaact tcgatctgcg gcatgccgcg cggtgccggg ttgataccat
     2221 ccaggttgaa ctgacccaga gatttgttgt cagccgcacg tttacgttca ccctgcagca
     2281 catggatggt taccgcagac tggttgtctt cagcggtaga gaacacctgg ctgtgcttgg
     2341 tcgggatagt ggtgtttttc gcgatcagcg tcgtcatcac accgcccatg gtttcgatac
     2401 ccagagacag cggggtaaca tccagcagca gtacgtcttt tacgtcacca gtcagaacac
     2461 caccctgaac agcagcaccg attgctacag cttcgtccgg gttaacgtct ttacgcggct
     2521 ctttaccaaa gaactcagca actttcttct gaaccattgg catacgagtc tgaccaccaa
     2581 cgaggataac gtcgtcgata tcagatacgg acaggccagc gtcctgcagc gcaactttca
     2641 gcggctcgat ggaacggtta accagatctt caaccaggct ttccagtttc gcacgagtca
     2701 ctttgatgtt catgtgtttc ggaccggtcg cgtctgcagt gatgtacggc aggttaacgt
     2761 cggtctgctg agcggaagac agttcgattt tcgctttttc tgccgcttct ttcaggcgct
     2821 gcattgccag cggatcgttg cgcaggtcaa tgccctgatc tttcttgaat tcttcaacca
     2881 gatagttgat cagacggctg tcgaagtctt caccacccag gtgggtatca ccgttggttg
     2941 ccagaacttc gaaggttttt tcgccgtcaa cttcgtcgat ttcgataata gaaatatcga
     3001 aagtaccacc acccaagtca taaaccgcga tagtacggtt gccagtacct ttgtccagac
     3061 cgtaagccag cgcagctgcg gtcggttcgt tgatgatacg ttttacttcc agaccagcga
     3121 tacggcctgc gtctttggtt gcctgacgct gagcatcgtt aaagtatgcc ggtacggtga
     3181 taacagcttc agttaccggt tcacccaggt aatcttcagc ggttttcttc atttttttca
     3241 gcacttcagc agaaatctgc ggcggtgcca ttttctggcc tttaacttcg acccatgcgt
     3301 cgccgttatc agcagcaata attttgaacg gcatgatgga aacatcacgc tgtacttctt
     3361 cgtcctggaa gcggcggcca atcaggcgtt taatcgcaaa cagggtgttt tgcgggttcg
     3421 tcactgcctg acgtttagcc ggctgaccaa ccagagtttc accatcctgg gtataggcaa
     3481 tgatagaagg cgtggtgcga tcgccttcgg cgttctccag cacacgagga gtggtgccat
     3541 ccataatcgc tacacaagag ttggtagtac ccaggtcgat accaattatt ttacccatct
     3601 aaacgtctcc actatatatt cggtcatcat gtggttgtga gtctgtaata ggggcgaaac
     3661 gtctggtttc aactgcccaa tttcattttt ttgcagactc actcactgcg gttgactact
     3721 aaatggggtc gtaacccacg tcatcaaggg ggagatgcaa aaattttttg tgcttttgcg
     3781 cagaaatttc gcgatatggc aggacaagac gtaaaattga cagcaatttt attcatataa
     3841 agaatgaatc gcagtcaagg tattcatcaa aaaattgtaa taaaaagaaa agattacgtg
     3901 cctgaatctt ctctttatca gcagtaaact agtgggtatt catcccccta cctcttccca
     3961 ctaagagaat ccttatgaaa tccgttttta cgatttccgc cagcctggcg attagcctga
     4021 tgctgtgctg cacggcgcag gcaaacgacc ataaactcct cggcgtcatt gcaatgccgc
     4081 gtaacgaaac caacgctctg gcgctgaaac ttcctgtttg tcgcattgtg aaacgcatac
     4141 aactctctgc cgaccatggc gatttacagt taagcggtgc atcggtttat ttcaaagccg
     4201 cccgtagcgc cagtcagagc ctgaatattc cttcagaaat aaaagaaggg caaaccactg
     4261 actggatcaa cattaacagc gataacgaca ataaacgctg cgtctcaaaa atcacctttt
     4321 ccggtcatac ggtgaactca tcggatatgg ccacgctgaa aattatcggc gacgactaac
     4381 gccagaatat agaagccaca aaaaatgaat gttaattacc tgaatgattc agatctggat
     4441 tttctccagc attgtagtga ggaacagtta gcaaatttcg cccggctgct cacccataat
     4501 gaaaaaggca aaactcgcct ctccagcgta ctgatgcgca acgaactgtt taaatcgatg
     4561 gaaggacatc ccgagcaaca tcgccgcaac tggcagctga ttgctggaga attacagcat
     4621 tttggtggcg atagtatcgc caacaaactg cgcggacacg gtaaattgta tcgggccatt
     4681 ttgctcgatg tttcaaaacg attgaagctg aaagccgaca aagagatgtc tacgtttgaa
     4741 attgagcaac agttactgga acaatttctg cgtaatacct ggaagaaaat ggacgaggaa
     4801 cataagcagg agtttctgca cgcggtcgat gccagggtga atgagctgga agagctgctg
     4861 ccgctgctga tgaaagacaa attattggca aaaggcgtgt cgcatctgct ttccagccaa
     4921 ctgacccgca ttttacgcac ccacgcagca atgagcgtac ttgggcatgg tttgctgcgc
     4981 ggcgcggggc tgggaggccc tgtaggcgcg gcactaaatg gggttaaagc ggtcagcggc
     5041 agctcctatc gcgtgacgat tccagccgta ctgcaaatcg cctgcctgcg ccggatggtt
     5101 agcgccactc aggtctgata ccggaaaccg gggaaaattt tcattttccc ctcaaaagat
     5161 cgtagacact gccccactgg ctgattatta tgccgcgccc tgaaaacact acagttattc
     5221 agggaaatta tttcaccatt cattcgatga tgatttttga ggaattatgg gcaacactaa
     5281 gttggctaat ccggcaccgc tgggcctgat gggcttcggc atgaccacca ttctgcttaa
     5341 cctgcacaac gtaggttatt tcgctctgga cggtattatt cttgccatgg gcattttcta
     5401 cggcggcatc gcgcaaattt ttgctggtct gctggagtac aaaaaaggca acactttcgg
     5461 tttaaccgca ttcacctctt acggttcttt ctggctgacg ctggttgcga ttctgctgat
     5521 gccgaaactg ggtctgaccg atgcgccaaa tgcacagttc cttggtgtct acctgggtct
     5581 gtggggcgta tttacgctgt ttatgttctt cggcacgctg aaaggcgcac gcgttctgca
     5641 attcgttttc tttagcctga ccgtgctgtt tgccctgctg gcgatcggta acattgccgg
     5701 taacgccgca atcatccact ttgccggctg gattggtctg atctgcggtg ccagcgcaat
     5761 ctatctggcg atgggtgaag tactgaacga gcagtttggt cgcaccgttc tgccgattgg
     5821 tgaatcccac taattgtctt gagatccccc cgctcggggg gattttttta ttcgctaacg
     5881 tcgcgtcttg cactcttcgg tctgaatgca gcaaccactt ctggtgccgt ttcaacgtat
     5941 ggcccttcca gcaactgaat gcagtacggt acgctggcaa aaataccgtg taccacaacg
     6001 ttaccctcag cgtccttcac accttccagc gtctctttaa tagacttcgg ctgaccgggt
     6061 aagttaagga tcagcgcctg tttgcgaatc acacccacct gacgcgaaag gatcgcagtt
     6121 ggtacaaaat gcaggctgat ctggcgcatc tgttcaccaa agccaggcat ctcgcggtcc
     6181 gctactgcca gcgtcgcatc gggcgttacg tcacgacgcg ccgggccagt tccgcccgtg
     6241 gtgagcacca gatggcaact catttcatcc accagctcac acaacgtttg ctcgatgatc
     6301 gcctgctcat cggggattaa gcgggtttcc agttcaaacg gcgtggttag cgccgatgtc
     6361 agccattctt ccagcgcagg gataccttta tcctgataaa cgccgctgga tgcgcgatca
     6421 gagatggaaa ctaagccaat acgtaaagta ttcatcaaga tttttccgtt taaacagtct
     6481 cgttaaacag aatgatacac tgcgaaggga gtgacagaca ggcttcagaa gaggtagcgt
     6541 gaccgacttc ccggtcacgc taagaatgat tacagcagat cgccgatcat tttttccagt
     6601 ttttcctggt caatagcaaa cttacggata ccttccgcca gtttatctac tgccattgga
     6661 tcctggttgt gctgccacag gaactcggac tcagtgatac gcgccggacg cgctttcact
     6721 tcgccggtgt aagacagttt acgttcgata gccccttcgc tctcagccag ctctttcagc
     6781 agtgccggtg cgatggtcag acggtcgcag cctgccagtt ccagaatttc gccgatgtta
     6841 cggaagcttg cgcccataac cacggtttca taaccgtgct ctttgtagta ctggtagatt
     6901 tcagatacag aaaccacgcc cggatcttct gccggagcgt actctttctt atcggtattc
     6961 gctttgtacc agtcaagaat acggccaaca aacggcgaga tcaggaacac gcccgcttcc
     7021 gcacaagcac gagcctgagc gaaggagaac agcagggtca ggttacagtt gataccttct
     7081 ttttccagct gttccgcagc acggataccc tgccaggtag aagccagttt gatcagaata
     7141 cgatcgttgc taatacctgc atcgttgtag agtttgatca ggcgttttgc tttcgcaatt
     7201 gacgcttctg tgtcatagga aagacgcgca tcaacttcag ttgagatacg gcccggaacc
     7261 agtttcagga tttccagacc aatatttact gccagtttgt cagtcgcgtc cacgatctgc
     7321 tgcgcgcgat cgttgctctg ctgtttcgcc caggcgacag catcatcaat caacttacgg
     7381 tattccggaa tctgcgctgc gttaagaatg agagaagggt tggttgtggc atcctgcggt
     7441 tgatacagct tcattgccgc gatgtcccca gtgtcggcca ctacggtggt gtactgacga
     7501 agggaggtca atttgtccgt catgatagta tttctcttta aacagcttgt tagggggatg
     7561 taaccggtct gccctgatga tatcacgacg caacgtcagc gcaaccgccg acaagacggt
     7621 tatcgccagg cgagactgtt tcggatttct gacactgcgt tgatatcgcc cgccattttt
     7681 aaacaaaacc tcatgtatgc tacgcagaag ttatcaagta cctcgtagcg tatatacttc
     7741 ttaaacaatt ggtaacgttt acacaggaaa gtcatcgcga ccggcaataa gagggatatg
     7801 catgccagat tttttctcct ttattaacag cgtcctttgg ggatcggtaa tgatttacct
     7861 gctcttcggc gcaggttgtt ggttcacttt tcgcaccgga tttgtgcagt ttcgctacat
     7921 ccgccagttt ggcaaaagtc ttaaaaatag cattcatcca cagccaggcg gtttaacctc
     7981 atttcagtca ttgtgtacca gtcttgcggc gcgcgtgggt agcggcaacc tggccggcgt
     8041 tgcgctggct attaccgccg gtggacctgg agccgtcttt tggatgtggg ttgccgcgtt
     8101 tatcggcatg gcgacctcgt ttgccgaatg ttcccttgca caactttata aagaacgtga
     8161 cgtcaatggg cagtttcgtg gcggaccggc atggtatatg gcgcgcgggc tggggatgcg
     8221 ctggatgggc gttctgttcg ccgtcttttt gctcatcgcc tacggcataa ttttcagcgg
     8281 agttcaggca aacgctgttg cccgagctct gagtttttct tttgattttc ccccgctggt
     8341 gacaggcatt attcttgctg tctttgctct gctggcaatc attcgcggtc ttcatggcgt
     8401 cgcccggctc atgcaggggt ttgtcccgtt gatggcgata atctgggtac tgaccagcct
     8461 ggtgatttgc gtaataaata tcgggcaact tccccacgtc atttggtcta tttttgagag
     8521 tgcttttggc tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat
     8581 tactaacggt tttcagcgca gtatgttttc caatgaggcg ggaatggggt cgacgccaaa
     8641 cgcggcagcg gcagcggcgt cctggcctcc gcatccggca gcgcaaggaa ttgtccagat
     8701 gattggcatt tttatcgaca ccctggtcat ctgtacggca agcgccatgc tgatattact
     8761 ggcgggtaac ggcacaacct acatgccgct ggaaggtatt cagcttatcc agaaggcgat
     8821 gcgggtgcta atgggttcct ggggtgctga atttgttacc ctcgtggtta ttctgtttgc
     8881 cttcagctcc atcgttgcca actacattta tgccgaaaac aatctcttct ttttacgcct
     8941 gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg
     9001 caccttgcta agtcttccgc tgatgtggca actggcagat atcataatgg cctgcatggc
     9061 tattaccaat ttgactgcca tattactgct ctcgcctgtg gtttatacca ttgccagtga
     9121 ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc gttatccgga
     9181 tatcggtcgc cagctttctc gggacgcgtg ggatgatgtt tcgcaggagt aatcacaact
     9241 atcgatcaac tcattctctt tttttgctaa agtcggcata aatttcctgc aaggactgga
     9301 tatgctgatt cttatttcac ctgcgaaaac gcttgattac caaagcccgt tgaccaccac
     9361 gcgatataca ctgccggagc tgttagacaa ttcccagcag ttgatccatg aagcgcggaa
     9421 actgacgcct ccgcagatta gcacgctgat gcgtatcagc gacaaactgg caggtatcaa
     9481 cgccgctcgc tttcatgact ggcagccaga tttcacgccg gagaatgccc gccaggcgat
     9541 tctggcgttt aaaggtgatg tctacaccgg cttgcaggcc gaaaccttca gcgaagacga
     9601 tttcgatttt gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactccgccc
     9661 gctcgattta atgcagcctt atcgtctgga aatggggatc cgtcttgaga atacccgagg
     9721 gaaagatctg tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc
     9781 agcacaaggc gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa
     9841 gccgaagaaa ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa
     9901 atttaagata atcagcttct acgctaagaa agcacgcggt ctgatgagtc gtttcattat
     9961 tgaaaatcgg ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt
    10021 tgatgaagct tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt
    10081 ttgtagtcaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgcagatt
    10141 caatatattg cagaattcac gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg
    10201 aatgccggat tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc
    10261 gccgtgatga tcatgatgag ctttcttatg gtgacgcggc ggtggcggcg gtccgtgtgg
    10321 gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg
    10381 accgccatcc cagtaataac cgcgattatc acgatcgcct atctgtaatt ttactgacgg
    10441 gactaacgta atttccgcag cctgcgttgc catgggagcg accagaacca gggaaagtgc
    10501 gagtacgata gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt
    10561 cgatatccgg agattacgcg atgagagcgg cggttgttat tctccgcaat cctaatctct
    10621 aagttaccgc atttattgag aatttctcct ttttccctgt cgtttctctc catattttct
    10681 tcacaaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagatg
    10741 ttactgatga ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgcga
    10801 aagcaagggt aaatcggcac gttctgccag ctcttttggc agatccaacg tttcaccgag
    10861 aatcgcttcc acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata
    10921 ttcgcctgga ttcaactggt cacgcagcgc acgatacgct acggcagcgt gcggctccga
    10981 agtgtagccc agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc
    11041 ggcataaccc agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg
    11101 ccagttgttc ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg
    11161 tgaccactga ccgtcatgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat
    11221 aaaacgtttc accggcagac cgagtgactt cgccagcaga cccgccgtca aatcgccgaa
    11281 gtttccgctt ggcaccgaga caaccagctg gttgcgcgct tcctgcggca gctgcgcaac
    11341 tgcttcaaag tagtagcaaa tctgcgccag caaccggcta atgttaatcg agttagctga
    11401 gtttaacccc agcgccactt tcagctcttc atcatcaaac gcctgcttca ccagcgcctg
    11461 acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca atgtacagaa
    11521 cagtttttct tgcagtggac tgattttgcc tcgtggataa aggataacca ctttcacatt
    11581 cggtaaaccg tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag
    11641 aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc
    11701 gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc
    11761 aacattggcg accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc
    11821 ctgcgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc
    11881 cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcgt gcggaaaaaa
    11941 cagcccctga tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcatt
    12001 gtgatcttta agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc
    12061 cagccggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc
    12121 ggcaacgcgc tgggcggtat ccggcttgtc acacagagca aacaaggtcg ggccggagcc
    12181 ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc
    12241 aggcagtaac cgttcacggt agggttctgc gataacatct ttcatcagct tcgcggcaag
    12301 ctcaggctga cgggaatagc aggcgtgaat gaagcctgcc aagtgtcgcc cgtgcgcaat
    12361 gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg tcgagacttt
    12421 aatccccgga tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat
    12481 gatgtcgttt tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc
    12541 gtaatgaatg ctgccggaga ttcgtccttc cagctcgccc atcaaagcca gcaaacgggt
    12601 gtcattaagc ggcttgccgc agtgttcatt catcgccatc agagccgcga ccaccgaaca
    12661 ggcgctggag cctaagcccg aaccgatcgg catattcttt tccagagtca tcgccactgg
    12721 aatttgcttg cccagctcct ggcaaaaacg ctcccagcac tgataaacga tattttcccg
    12781 tggttctgac ggcagcttat cggcaaagcg tccgaggttg ttgagactga atgtctctgc
    12841 cgactcaacc gtgactacat ctccgagcaa tgcaccatca acgggtgtca ccgccgcccc
    12901 gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat
    12961 gtcagactcc taacttccat gagagggtac gtagcagatc ggcaaagaca ccggcagcgg
    13021 taacgtcatt gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc
    13081 tataaaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca ttaccatcca
    13141 cttcggcaat cttcacgcgg cagacgccat cttcatcaat attgccaaca tagcgcaaaa
    13201 cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca
    13261 gattcgccat aaaagcggca acatcaccct cagcgttaaa ctctgcgggc agcacaggtt
    13321 caatttcaat atccgccagc tccagttcac gtcccgtttc acgagcgaga attaatagtt
    13381 tacgcgctac atccatacca gaaagatcat ctcgcggatc cggttcggta taacccattt
    13441 cccgcgccag cgtagtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat
    13501 aagaaagcga acctgaaaga atgccggaga acttcatcaa ttcatcacca gcattgagca
    13561 gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt
    13621 tacgccgcga tttttcagcc gcatgacgca acagatggta gtaatccatc gacgaggtgt
    13681 tggccttttt gttcggcgtg acaacgtgga aaccttcgcg caggaagtcg gcatattgat
    13741 ccgccactgc ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt
    13801 tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgcgcc agttcttcct
    13861 gccagttttc cagatttagg ccatgcacat tggtgagcag agccttcgag ttagcaacac
    13921 cgcagacacg taagtcgata tgtttattct tcaaccagct ttgctgacgc ttcagttgct
    13981 ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg
    14041 tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca
    14101 cgacagagat tgagcgttca gaagatccct gagcaatagc gacaatgttg atattggcgc
    14161 gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta
    14221 ccaccgagat aatggccagc cgttccgtca ccgccagcgg ctccagtaag ccttctttca
    14281 gttccaggta gaactcttcc tgcattgccc gttcagctcg cacacagtcg ctttgcggaa
    14341 cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg
    14401 cgcgtgacat tgcagcaaag acgcgcgccg ccatgccgac cattcctttc atccccgggc
    14461 cggaaacgct gaacattgcc atattattca gattggaaat gcccttgacc ggtaattcgt
    14521 cttcatcacg actggcacca atgagcgtac ctggagcttg aggatttccg gtatttttaa
    14581 tcaggcaagg gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag
    14641 cgccgaagta ggaaagctcc atcgcttcct ggtaggacat cgatttcaac aacctcgcat
    14701 cgggcacctg acgcgggtcg caggtatata ccccgtcaac gtccgtccaa atctcgcaac
    14761 aatcggcgcg taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa
    14821 gtaccaccag ttcgcctttt tcattaccgg cggtgaaacc tgccatcagc accatgtgat
    14881 cagccggaat acgacttgcc gcaatacggc gggtggactc tgcaatatcg acagtagatt
    14941 cgaggtaatg ccccactgcc agcagttttt cgaccggatc gataacggta acgttgtgac
    15001 cgcgcgcttc taatacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca
    15061 gcgcagcgtt aatgctatcc gggcactgcc ctaacaaact aatgccatgc aggacatgtt
    15121 ttatttgggc aaattcctgg tcgacgaaag ttttcaattg cgccagcggg aatcccggct
    15181 gggcggcggc gagtcccgtc agaagttcgg caaaaatacg ttcggcatcg ctgatattgg
    15241 gtaaagcatc ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt
    15301 tggcgggggc agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat
    15361 cggcaacccg cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc
    15421 gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc
    15481 ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa tggtaatggt
    15541 gatggtggtg gtgatggtgg tggtaatggt ggtgctaatg cgtttcatgg atgttgtgta
    15601 ctctgtaatt tttatctgtc tgtgcgctat gcctatattg gttaaagtat ttagtgacct
    15661 aagtcaataa aattttaatt tactcacggc aggtaaccag ttcagaagct gctgtcagag
    15721 actctttttt taatccacac agagacatat tgcccgttgc agtcagaatg aaaagctgaa
    15781 aaatacttac taaggcgttt tttatttggt gatatttttt tcaatatcat gcagcaaacg
    15841 gtgcaacatt gccgtgtctc gttgctctaa aagccccagg cgttgttgta accagtcgac
    15901 cagttttatg tcatctgcca ctgccagagt cgtcagcaat gccatggctc gttcgcgtaa
    15961 agcttgcagt tgatgttggt ctgccgttgt gtcacttttc gtcggttgtt gtattaatat
    16021 tgctaattga tagcaataga ccatcaccgc ctgccccaga ttgagcgaag gataatccgc
    16081 caccatcggc acaccagtaa gaacgtcagc caacgctaac tcttcgttag tcagcccgga
    16141 atcttcgcga ccaaacacca gcgcggcatg gctcatccat gaagattttt cctctaacag
    16201 cggcaccagt tctactggcg tagcgtagta atgatatttc gcccgactgc gcgcagtggt
    16261 ggcgacagtg aaatcgacat cgtgtaacga ttcagccagt gtcgggaaaa ctttaatatt
    16321 atcaataata tcaccagatc catgtgcgac ccagcgggtg gctggctcca ggtgtgcctg
    16381 actatcgaca atccgcagtt cgctaaaccc catcgttttc attgcccgcg ccgctgcccc
    16441 aatattttct gctctggcgg gtgcgaccag aataatcgtt atacgcatat tgccactctt
    16501 cttgaccaaa tgaccgcgaa cagggtgatc actgtcaact tattacgcgg tgcgaattta
    16561 caaattctta acgtaagtcg cagaaaaagc cctttactta gcttaaaaaa ggctaaacta
    16621 tttcctgact gtactaacgg tttaattgtt aaaaaatgct acatatcctt ctgtttactt
    16681 aggataattt tataaaaaat aaatctcgac aattggattc accacgttta ttagttgtat
    16741 gatgcaacta gttggattat taaaataatg tgacgaaagc tagcatttag atacgatgat
    16801 ttcatcaaac tgttaacgtg ctacaattga acttgatata tgtcaacgaa gcgtagtttt
    16861 attgggtgtc cggcccctct tagcctgtta tgttgctgtt aaaatggtta ggatgacagc
    16921 cgtttttgac actgtcgggt cctgagggaa agtacccacg accaagctaa tgatgttgtt
    16981 gacgttgatg gaaagtgcat caagaacgca attacgtact ttagtcatgt tacgccgatc
    17041 atgttaattt gcagcatgca tcaggcaggt cagggacttt tgtacttcct gtttcgattt
    17101 agttggcaat ttaggtagca aacatgcaga ccccgcacat tcttatcgtt gaagacgagt
    17161 tggtaacacg caacacgttg aaaagtattt tcgaagcgga aggctatgat gttttcgaag
    17221 cgacagatgg cgcggaaatg catcagatcc tctctgaata tgacatcaac ctggtgatca
    17281 tggatatcaa tctgccgggt aagaacggtc ttctgttagc gcgtgaactg cgcgagcagg
    17341 cgaatgttgc gttgatgttc ctgactggcc gtgacaacga agtcgataaa attctcggcc
    17401 tcgaaatcgg tgcagatgac tacatcacca aaccgttcaa cccgcgtgaa ctgacgattc
    17461 gtgcacgcaa cctgctgtcc cgtaccatga atctgggtac tgtcagcgaa gaacgtcgta
    17521 gcgttgaaag ctacaagttc aatggttggg aactggacat caacagccgt tctttgatcg
    17581 gccctgatgg cgagcagtac aagctgccgc gcagtgagtt ccgcgccatg cttcacttct
    17641 gtgaaaaccc aggcaaaatt cagtcccgtg ctgaactgct gaagaaaatg accggccgtg
    17701 agctgaaacc gcacgaccgt actgtagacg tgacgatccg ccgtattcgt aaacatttcg
    17761 aatctacgcc ggatacgccg gaaatcatcg ccaccattca cggtgaaggt tatcgcttct
    17821 gcggtgatct ggaagattaa tcggctttac caccgtcaaa aaaaacggcg ctttttagcg
    17881 ccgtttttat ttttcaacct tatttccaga tacgtaattc atcgtccgtt gtaacttctt
    17941 tactggcttt cattttcggc agtgaaaacg cataccagtc gatattacga gtcacaaaca
    18001 tcatgccggc cagcgccacc accagcacac tggttcccaa caacagcgcg ctatcggcag
    18061 agttgaggag tccccacatc acaccatcca gcaacaacag cgcgagggta aacaacatgc
    18121 tgttgcgcca acctttcaat accgcctgca aataaatacc gttcattaac gccccaatca
    18181 gactggcgat tatccatgcc acggtaaaac cggtatgttc agaaagcgcc agcaagagca
    18241 aataaaacat caccaatgaa agccccacca gcaaatattg cattgggtgt aaacgttgcg
    18301 cggtgagcgt ttcaaaaaca aagaacgcca taaaagtcag cgcaatcagc agaatggcat
    18361 acttagtcgc ccggtcagtt aattggtatt gatcggcggg cgtcgtcact gcgacgctaa
    18421 acgcggggaa gttttcccag ccggtatcat tgcctgaagc aaaacgctca ccgagattat
    18481 tagcaaacca gctgctttgc cattgcgcct gaaaacctga ctcactaact tcccgtttgg
    18541 ctggtagaaa atcacctaaa aaactgggat gcggccagtt gctggttaag gtcatttcgc
    18601 tattacgccc cgcaggcacc acagaaagat cgccggtacc gcttaaattc agtgccatat
    18661 tcagcttcag gttctgcttc cgccagtccc cttcgggtaa agggatatgc acgccctgcc
    18721 cgccttgctc taacccgatg ccgggttcaa tagtcagcgc cgttccgtta acttcaggcg
    18781 ctttcaccac accaatacca cgcgcatccc cgacgctaat cacaataaat ggcttgccaa
    18841 aggtgatatt tggcgcgtcg agttcgctaa gacgcgaaac atcgaaatcg gcttttagcg
    18901 ttaaatcact gtgccagacc tgaccggtat aaatccctat cttgcgttct tccacgttct
    18961 gattgccatc aaccatcaat gactcaggta accaaaaatg gataaaactt cgtttccgct
    19021 ccacggtttt atcctcttcc tgcaccgtat aaagctcggt cacaggaata gcgataagag
    19081 gcccaacgag tttttgcggc ccgctggtac tttggcgaat cgcatcttcc acatcgctac
    19141 ggtaatcggc acgttcgaca atcacctgcc gaatcagcat tatcggaatc aacaacagca
    19201 atactgcacc aaacagggtg gtcattttcc agaacagggg ggatttcaac atagtcttct
    19261 cctttgcaat ggcagcagga tacgaaggct atgtgggaag aatttgaagc tatgtgaagt
    19321 gacggtgaag ccgaagcgag gccagcacgc caccttcctg cacgttgcgc agcgtgactt
    19381 cgccgttaaa caaacgggcg acctcactga caaacgccaa ccccagaccg ctgctttttt
    19441 gcccatttgc acgcggcaaa gagtaaaagc gttcaaaaat acgtgacagc gcgtagtcag
    19501 gaataccact accggtatcc agcaccttaa gggcgacgtg ttcctgatcc acttcggcgc
    19561 ttagcgttat gcgaccgctc ttgggggtaa aatcgatggc gttatccagt aaattcccca
    19621 gcgcctgctc cagtaacgcc ggttcagcag caacgttaac ctcagtaggc ataacatgca
    19681 aagtgatgtt tttttctgcc aactgcacgg tgcgcgcttc gctgacgcgg cgaaataatg
    19741 ccgccacatc aacagcagtc agaacgactt cctgacgatt ctccagtctt gcctggcgta
    19801 gtaacgtttc taccaatgcc tgcatacgcg cattttgcgt cagaatgttg tcggtaaaac
    19861 gagccaccac ttccggtggc ggaccttcgc gtaaaatttc cgccgcgcca cgaatcgccg
    19921 ccagtgggct ttttagctca tgggttaacg cataaacata ctgctcaata tagtttttcc
    19981 cttccagctt cacgcgcata ctttccagcg cctgcgcaag cttacgcaac tcgctactgc
    20041 cgagttcagg aagaggaacg ggcttattgt cagtgacgga atcggcatag cgagtgagcc
    20101 gggcaatgga gcggttgatc caccaaacca tgcctgcgcc aatcaccagt gcaatcccca
    20161 gcaaaatggc gctggcccat aaaattcgcc gctcgctgcg cttaatgacc ggagccatcg
    20221 ccgcgttcgg tttgcctacg ctcaaaacgc caataagccg cgagccgtcc ataatcggcg
    20281 cggcaacata catcaccgaa ctttcgggat cggcaggatt ttgcaacgtg ctgcgcgcac
    20341 catactgacc acgcaacgtt agccagacgt cattccagcg cgaataatcc tgcccaacgg
    20401 ctttatttgc cgaatcgaac aatactttgc cctgcgcatc ggtcatatag acatgatatt
    20461 cattgcgcac tttgttaatg ccaccgatat tagcgcgaaa cgggcgatgt tgtagctgat
    20521 taaacgcctg cgccagttgc ccatgcgttg ggtccccaga gagcaaatcg ggacgcgcca
    20581 gctccgccag caacgttgcg gtgtcgatca gcgtcccctc cgttgctctt cgcacgcccg
    20641 gtttaacttc tttgacaaaa atagccagta cgaaccaggc cgccaccgcc accagtaaaa
    20701 aatagcccag caacaaccgc atgccgatac gcattacagg ctcctcaggc tgtatcccat
    20761 gccgcgatga gtattaatcg gtgaaagatc ggggttgatg gcgcgcagct tggcacgcag
    20821 cgttttaatg tgggtatcga cggtgcgatc gtaggtgtcc tgcgcatctt cccacacgat
    20881 atccatcagt tgctggcggg accagacgcg gcctggcgac ttgagtaacg tcttcagcaa
    20941 taaaaactca taccgcgtca gcgttaatgg cgtgtcaaac cagctgatct gcgccgcggg
    21001 ttcattcaat tcaaaatgac caatacggat gacgggagac ggcgtcgaga acttcttcac
    21061 ccgacgcagc aaggtgcgca ccctagcgca cacttcgcgg ggtgaaaacg gtttagccac
    21121 atagtcgtca gcgccaattt ccagcccaag caggcgatcg acctcttcac ttcgggccgt
    21181 caggaacagt acaggtaacg ccggatggag cgccagtaac tggcggcaca attcaaagcc
    21241 actgatatca ggcaaaccaa cgtcgagaat catgacatcg ggaacctgct ggcgagcttt
    21301 atccagcacc ggcaagcctc gctcaaagac ctcgacggca aaaccttcct gctgcaacat
    21361 gtagaccagc gtgtcggcta tcccttgctc atcttccact aaccagaccg tttcccgttg
    21421 cataaatcgc ctctgttatt gtcgccacgg catgacagga accgcgctaa tcgcgttttt
    21481 cggcgaacct tctacaactt tgtcggagta agccagataa gcgagcgcgt tgcgtttggc
    21541 gtcataaaag cgcacgacct gtaacgactt aaagaccagg gacgtgcgtt ttttgaatac
    21601 tacctcgccc tgagctttgc cgtttttaat acgatccgac agttcaatcg gcccgacttg
    21661 ctgacaagaa atagccgcat cggaggtatc ttccgccaga cccaatcccc ctttaatacc
    21721 acctgttttc gcccggctca cataacaggt gacatttttc acatcgggat cgtcaaaggc
    21781 ttccacaaca attttgtgat ccgggccgat cattttaaat acggtgtcga ccgaaccaat
    21841 ctcttctgca tgagccaatg gccccagcat tatcaggctt aaagacagga tcaaatgctt
    21901 gtatttcata ttgttaccat ttttatctat tacacgagca attagttcgt cacggtaaat
    21961 aacatattga ttaagaacaa aatctcaata cttttatttc cgtcaagacc taaaacatac
    22021 tctactaaga aaaaaacact gaatgctaaa acagcaaaaa atgctattat ccaattacct
    22081 gatgtcaggt gctcgttgtt gaaaggatga ggatatttta tggatcaggc cggcattatt
    22141 cgcgaccttt taatctggct ggaaggtcat ctggatcagc ccctgtcgct cgacaatgta
    22201 gcggcgaaag caggttattc caagtggcac ttacagagaa tgtttaaaga tgtcactggc
    22261 catgctattg gcgcgtatat tcgtgctcgt cgtttgtcga aatcggcggt cgcactacgc
    22321 ctgactgcgc gtccgattct ggacatcgcg ctgcaatacc gcttcgactc tcaacagaca
    22381 tttacccgcg cattcaagaa gcagtttgcc cagactcctg cactttaccg ccgttctcct
    22441 gaatggagcg cctttggtat tcgcccgccg ctgcgtctgg gtgaattcac tatgccagag
    22501 cataaatttg tcaccctgga agatacgccg ctgattggtg ttacccagag ctactcctgt
    22561 tcgctggagc aaatctctga tttccgccat gaaatgcgtt atcagttctg gcacgatttt
    22621 ctcggcaacg cgccgaccat tccgccagtg ctctacggcc tgaatgaaac gcgtccgagt
    22681 caggataaag acgacgaaca agaggtattc tataccaccg cgttagccca ggatcaggca
    22741 gatggctatg tactgacggg gcatccggtg atgctgcagg gcggcgaata tgtgatgttt
    22801 acctatgaag gtctgggaac cggcgtgcag gagtttatcc tgacggtata cggaacgtgc
    22861 atgccaatgc tcaacctgac gcgccgtaaa ggtcaggata ttgagcgata ctacccggca
    22921 gaagacgcca aagcgggaga tcgcccaatt aatctgcgct gtgaactgct gattcctatc
    22981 cgccgttaac gctgcagctc atctaatgca ggggcgtcca gatgcgagat gtcccctgcg
    23041 gtttcaacga cccagccaga cgccagccac aggctttcct gataatccac gcgcgaaata
    23101 gaacagttac gcagacgtaa gcggcgctct gcccatgctg gtaatccgag aatcgtactc
    23161 accagacatc ccagtgcaat accatggctc accagcagcg gtcggcttcc ctgcggtaag
    23221 tcccggcaag attccagtgc cgcattgacg cgatcgctga gctcctgcat tgactcgcct
    23281 tcaggaatac gcccatcaac ggtgccattg accagctgcc gacgccagtt ctcttcttct
    23341 tcggtcagag aatcgatatg tcttttttcc agcacaccca tgtttaattc acgcaggcga
    23401 gaatcaaaga tgatgtcaca gccgcaggcc tgggcgatga tttccgccgt acgccgggtg
    23461 cgtcctaaat cgctactgat gatatgcgta atgccaagct ctttggcacg cgtcgccacc
    23521 tgcatcgcct gttgctcacc tttggcggtc agcgggctgt cagactggcc ctgaatacgt
    23581 cgctcggcgt tccactgcgt ttcaccgtgg cggactaggt atacctgtaa catgcttttt
    23641 ttccgtatac tgctgtaacg ttaattttct gagtaatgct gattatgcac caagttgtct
    23701 gtgcgaccac caatcccgct aaaattcagg ccattctgca ggcatttcac gagatcttcg
    23761 gcgaaggatc ctgccatatt gcatccattg ccgtcgagag cggtgtaccg gaacagccct
    23821 ttggcagtga ggaaacgcgc gctggcgcac gaaaccgggt agccaatgcc cgccgtttac
    23881 ttccagaggc tgatttttgg gtggcgattg aagctggcat tgatggcgac agcactttca
    23941 gttgggtggt gattgaaaac accacccagc gcggcgaggc gcgttcggca actttgccgt
    24001 tgcctgcggt gatattggaa aaagtacgtg aaggtgaggc gcttggtccc gtgatgtcgc
    24061 gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg tttaccgccg
    24121 ggaaactcac tcgcgccagc gtctatcacc aggcggtgat cctggcgctg agtccgtttc
    24181 ataatgcggt gtattaagcg cctgatgcga cgctgccgcg tcttatcatg cctaccaaac
    24241 atattgaaat tacgggtatt tgtaggacgg ataaggcgtt cacgccgcat ccggcattcg
    24301 gtgcacgatg cctgatgcgc cacgtcttat caggcctaca aaatcaatcg cttttcagca
    24361 acacctcttc cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac
    24421 gcgtaatcgt cgcgatgcct gcgccgagtt cattttttaa ctcacgctgg ctcatttcgc
    24481 cgcgcaacag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg
    24541 tcagcatcag gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt
    24601 cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt
    24661 gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta
    24721 ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc
    24781 cgtggcgctc atcaacgtcg gtttatcccc catgaaatag cggtagtaag cgtcataagc
    24841 cagcacgttc ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac
    24901 agcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt
    24961 ataagctgct gaggagaaaa tacggttatt accaaattgc tgataaacat attgcaggta
    25021 actggtgcca atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc
    25081 aggaatagag aacatcttca ccgtatgggt cgccgtgccc ggcataatct gcatcaggcc
    25141 gctggcccct accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc
    25201 catcgcatag ctttgcggga tctccttccc actggtgtaa cgtttgaaaa gatcgttgta
    25261 agccagcggg aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac
    25321 gctaagatcc caccattggt tgttgaaagc ataccgcgcc agttgagcct gctctgtttt
    25381 tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta
    25441 catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg
    25501 cggcgcttta tcaatcttca gctcatactc ttcgccgatg cgttgtgcag caaccatcgg
    25561 gtagaaacca cgctgttgca tgagttgatg caaaatctct tttgcttcag cttcacgtcc
    25621 gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt tcgcttccat
    25681 aggcagacgc gccagccagg tattcaggcc gcgacgatcg ccagtgccaa gcgccattcg
    25741 cacacggcgt tcaataagcg aagtagattg cgagcgcata atggcgtcat cgcgccattt
    25801 cgcctgctcg tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc
    25861 ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gatgggatca tcagccgtgc
    25921 attttcagca tcctgccgcg ccacactggc aaacgccacc gccgccattt gacgggtaaa
    25981 atcggtcgct ccggttgtac gcgcgaaggt cagtaccgta ttggggttgt tcgccagtga
    26041 aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac
    26101 caggccagtg ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc
    26161 ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt
    26221 gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca
    26281 tttcgcatag tagtaattac attgtgcttc ggtagttccg ggcttttccg ggctaaaggc
    26341 taacaggcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt
    26401 gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg
    26461 attcatcagg tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt
    26521 cagtccaggc atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat
    26581 ttgcgcgtaa cgactacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt
    26641 cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag
    26701 cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta
    26761 accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact gcgagggtga
    26821 tcgaccggct gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc
    26881 acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc
    26941 ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct
    27001 ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtccaccct gctgcgcatt
    27061 atggcgggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt
    27121 ggttatctgc cgcaggaacc gcagctgaac ccggaacaca ccgtacgtga gtccattgaa
    27181 gaagcggttt cagaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac
    27241 gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc
    27301 attcaggctc acgacggtca taatctgaac gtacagctgg agcgtgcggc ggatgcgcta
    27361 cgtctgccgg actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgtg
    27421 gcgttgtgcc gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac
    27481 cacctggatg ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgaaggcacc
    27541 gtggtggcga ttacccacga ccgttacttc ctcgataacg ttgcgggctg gatcctcgaa
    27601 cttgaccgcg gtgaaggtat tccgtgggaa ggtaactact cctcctggct ggagcagaaa
    27661 gatcagcgtc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa
    27721 gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg
    27781 gcacgctttg aagaactgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg
    27841 tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa
    27901 tcctatggcg atcgtctgct gattgatgac ctgagcttct cgatcccgaa aggagcgatc
    27961 gtcgggatca tcggtccgaa cggtgcgggt aaatcgaccc tgttccgtat gatctctggt
    28021 caggaacagc cggacagcgg caccatcact ttgggtgaaa cggtgaaact ggcgtcggtt
    28081 gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg
    28141 ctggatatca tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt
    28201 aactttaaag gggttgatca gggtaaacgc gttggtgaac tctccggtgg tgagcgcggt
    28261 cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca
    28321 accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg
    28381 ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatt
    28441 ctggattacc aggatgaagg taaagttgag ttcttcgaag gtaactttac cgagtacgaa
    28501 gagtacaaga aacgcacgct gggcgcagac gcgctggagc cgaagcgtat caagtacaag
    28561 cgtattgcga agtaatgcgt aaaatgccgg atgcggcgcg aacgccttat ccggcctaca
    28621 aaaccagcaa tttcaatacg ttgtaaaact gtagacctga taagacgcgg taagcgtcgc
    28681 atcaggcatt gagcaccgaa tgccggatgc ggcgtgaaca ccttttccgg tctacaacag
    28741 ccttagcatc ctcaatttcg tggcgggcca cccgtgcagg ttttttcaaa ccttgtttaa
    28801 tctcgacagc ctcttccaga gaggttttca actcatcaaa aaaactcatt ttatacctcg
    28861 cctttgagca gtttcatcac ggttatctct gctcccccat catctcccgc accagctcca
    28921 cgcagcgcag gaaacggctg tcgtaatctt cctcttcaac ccgcacaaat tcgatattgt
    28981 tctcttccag catctccacc agcagattct ggaactcttt acgatccacc gaactgccga
    29041 ggctgcgtaa accatccacc acccacggcg tgttgttctc cagcaggatc accagatcga
    29101 aacggtattc atcaatcagc gcctgcacga acggatgttc ccgcccttcg tactttttgc
    29161 agaacgcctg agtggtgaca aaatcggtat cgataaatgc cactttattg gcatatttca
    29221 ccgcaaaatc aatgtattga gcgtggccca gcgcgatttt atcgtagtca gaatactgca
    29281 atgcgatctc atcaccgccg aggtgtgaaa agacataatc gcggccatat tcccacgcac
    29341 tggtggtgtt gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc
    29401 cgccaaggat cgccacggta cgcacaaaaa acggcttcac ttcggtagga atatattccc
    29461 agtagcggaa cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg
    29521 gatcgaccag caccgtctcg atccccagat gttccatata ctgtggcgca tcggcttctt
    29581 ccgaggtgta gatcagatcc ggctggatcc ccttttcagc cataaacttt ttgataccgt
    29641 tgctccacac atcccagccg tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa
    29701 tgcgaatatt tttctgatat ttaaaagttt gcaataacca gcgcagacga tccggcacgg
    29761 tcggctgctg cgacatggca ctgtcttcga acaacgcgcg gtcacgggta tcgtcaaaac
    29821 ccataatgat atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc
    29881 cggtatgcag tgggtagaac ttaccgaata cgacaccgat cgttttcttc tgccggggaa
    29941 actcaagccc caaaaaacgg tgcaacgcct ccagcttttg cgcgctgggg cttttgattt
    30001 tggcattcag taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct
    30061 gtagcgtgca gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc
    30121 cctccgtatt tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac
    30181 gccaaaaatc tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc
    30241 ccggcgaaaa ccgtgtttcg ccgcttcaga gattcgttcc tgaccgctgg gcaccgggcg
    30301 gatctcccct gccagcccga cttcaccaaa caccaccaga tcctgcggta gcggtctgtc
    30361 acgcaggctg gaaaccatcg ccagcagtaa cgctaagtcg gcgctggttt cggttacctt
    30421 tacgccgccg accacgttca caaacacatc ctgatcggcc atttgcagac caccgtgacg
    30481 gtgcaagaca gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg
    30541 cggattcgcc atcatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt
    30601 tccttcccac accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa
    30661 aattgccgaa gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag
    30721 ctcattcacc gcgccgaagc ggtttttatg gctgcgcaag gtgcgaaaac gggagtcggc
    30781 atcgccatct aaaagcaccg aacagtcgat acagtgttcc agcactttcg ggccagccag
    30841 cgaaccatct ttggttacgt gccccaccat gacaatcgcc acaccgcgcg ttttggcgaa
    30901 gcgtgtcaaa taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgactgtac
    30961 atccgccata tgcatcacct ggatcgagtc aattaccatc agcttcggtt gctcttcttc
    31021 ggcaatcagg cagatctgtt cgatgctggt ttccgacaac atattgagat tgtcagtcgg
    31081 caggccaagg cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata
    31141 cagcgttttc atctgctggg ccagtttgca cagcgtttgc agtagcagcg tggatttccc
    31201 cgcaccaggg ttaccgccaa tcagaatggc acttcctggc accacgccgc cgcctagtac
    31261 gcggtcgaac tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga
    31321 gagtttctgg acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg
    31381 cgccaccgta ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc
    31441 actgcactgc ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg
    31501 ttttggagct tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatggcgggc
    31561 gagcaattac ttctgattca ggctgcctga gaggatgcag aataccccca tcaggtcagc
    31621 gtgacggatg gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc
    31681 cagccctgcc gctttgatca tcggcaggtc attggctcca tcgccaatcg ccacggtctg
    31741 cgccagcggg atttcatact cctgcgcgag gcgagtcaga gttttcgctt tgtactgcgc
    31801 gtctacgatg tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt
    31861 ggctaccacg gctgtcaggc gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc
    31921 gccggaggca atcgccactt tccagcccag cgtttccagc ttgagcacca gttgcgttaa
    31981 gcctggcatc agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt
    32041 cagcgtcgcc acgcggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg
    32101 ttcggttact tccgccacca tctcgcccgt tccggccagt ttggcaattt catcaataca
    32161 ttcaatctgg atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg
    32221 cgggattttt cccagcggcg cgacatccag ctgcgcttcg tgggccaggc gtgtagcccg
    32281 tgcggtgagt gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac
    32341 aatcaccatc gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc
    32401 acgaccatac agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc
    32461 accacttaat gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca
    32521 ggtaatgtta ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga
    32581 ggctaccttg tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag
    32641 atggctcgca caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt
    32701 gccttgttag tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag
    32761 cgtaatccac agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt
    32821 gcaccgctga tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag
    32881 ttaacggatg aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc
    32941 gcacgttctg gcgaaagcgt cgaagtgcgc gatcggctgg cgctcgacgg taaaaaagca
    33001 ggcggctatt ttaaccagca gattgtcgag ccaattgccg ggaaaaacgg accgctcggc
    33061 tatctgcgcc tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac
    33121 accactaaca ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc
    33181 cgcacgctgc tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc
    33241 agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag agtgccccat tactacaaga
    33301 aaggaaatcg ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt
    33361 aacctggcgg tggaagagtg tatttttcgc caaatgcccg ccacgcagcg cgttctgttt
    33421 ctctggcgca atgccgacac ggtagtaatt ggtcgcgcgc agaacccgtg gaaagagtgt
    33481 aatacccggc ggatggaaga agataacgtc cgcctggcgc ggcgcagtag cggtggcggc
    33541 gcggtgttcc acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac
    33601 gataaaacca tctccacgtc gattgtgctc aatgcactga atgcgctcgg cgtcagtgcc
    33661 gaagcgtccg gacgtaacga tctggtggtg aaaaccgccg aaggcgaccg caaagtctca
    33721 ggatcggcct atcgcgaaac caaagatcgt ggcttccacc acggcacctt gctgctcaat
    33781 gccgacctta gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa
    33841 ggcattactt cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggatcccc
    33901 catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg cgagcgcgtg
    33961 gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt
    34021 gcccgccaga gtagctggga gtggaacttc ggtcaggctc cggcattctc gcatctgctg
    34081 gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc
    34141 acccgcgccc aggtgtttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg
    34201 cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg
    34261 gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg
    34321 gtaaggtaac tcgctgatta aaatatccaa ggaaggaaat atgatcgatc tggaaaacca
    34381 ggaacgggag attatcaatt taatgttcag ccagcgtatt tcctggctag ccgccgtgcg
    34441 tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc
    34501 tctaaaacag atcgaaaaga cggaacggct tagctcaaac atcaaaagca aaatggcaga
    34561 aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa
    34621 atgaatagat tcgtgaatag atacttgaat ggattttatt ttctatccat atgaaaaata
    34681 atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtcc
    34741 cccactccgg cttaacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc
    34801 agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg
    34861 gcagtggttt gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt cagggctttc
    34921 gcgccaaact ctgcagccac gccgtagata ccagccgctt ccatttccac gccgagaatg
    34981 ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag
    35041 aacaggttac caacgcgagc atcaatgccc agtgctttag ctgcatctac tgcgttacgc
    35101 accatgtcga agtcagcgat agcggcaaag tcatggtctt taaaacggat gcggttaact
    35161 ttggaatcgg tgcaggcacc cataccgata acgacgtcgc gcagttttac gtgcggcaga
    35221 actgcgccac aggaacccac gcggataatt ttcttcacgc cgaaatcggt gatcagttct
    35281 ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg
    35341 cctttgtaag taccggtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct
    35401 tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg
    35461 tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt atcctttttg
    35521 tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg gctttgccat
    35581 attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc gcgaaggttt
    35641 cacggtgacc cagtgagccc ggttttactt tcgggccgta aaccagtacc ggaatgtgtt
    35701 cacgcgtgtg gtcagtaccg gtccaggtcg gatcacagcc gtggtcagcg gtgaggatca
    35761 ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg aacagttcca
    35821 gacccgcagc ataaccggcg acgtcgcgac ggtggcccca ggaagagtcg aagtcaacga
    35881 agttggtgaa gacgatggtg ttatcacccg cttctttcat ctctttgatg gtggcgtcaa
    35941 acagcgcgtc caggccagtc gctttcactt ttttggtgat accgcagttg gcgtagatgt
    36001 ctgcaatttt accgaccgaa accacctggc cgtgtttttc atcaaccagt ttctgcagca
    36061 cggtcggtgc tggcggctca acagccaggt cgtgacggtt accggtacgc tggaagttac
    36121 cggctttgtc gccgataaac ggacgagcga taacacgacc gatattgtag ccgccgttgg
    36181 tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg aaagtttctt
    36241 catggcaggc aatctggaac acggagtcag cggaggtata gaaaatcggc ttgccggttt
    36301 tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg cagttaccga
    36361 ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc gggaagctgt
    36421 tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggca atttcccagt
    36481 gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac gcgccgataa
    36541 cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg tgtgctttcg
    36601 ccagccccag acgggtcaga tttggcagat tgagcgggcc tttacgaccg ttatcagctt
    36661 cgcctttggc acaagcttct gcgatatgac ccagggtgtc agccccgacg tcaccaaagc
    36721 gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata aatgcacgtt
    36781 tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta taccgttatt
    36841 cgctgatacg gcgatagaca gttggtgtgc tttccggtgc tttatcggca agtttaattg
    36901 ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg tggataaccg
    36961 ccagcggacg ctggccgtct acctggtcgc ccagacgtgc catatcagta aagccgacgc
    37021 tgtaatcgat ggtgtcagat gcctgacggc gtccgccgcc cattgcaacc actgccatcc
    37081 ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag actgctttcg
    37141 tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc gggccttttt
    37201 gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc accgcctgca
    37261 atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagataagc atctccacac
    37321 acagcgccat cgtgacatca aacagacgcg ggttacgata ttcacccgtc aggaactgca
    37381 ccgcttcacg tacttcaacc gcgttaccgg cactggaggc cagcacctga ttcatgtcgg
    37441 tgagcagtgc ggtggtgcgc acgcctgcgc cgttagccac gccaacaatc gcttcggcaa
    37501 gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc acgtccatca
    37561 ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg atcagcggga
    37621 tggagtccac ggttgcggta atatcacggg tcgcgtagaa acgtttatcc gccggagcca
    37681 gtgagctggt ctgaccgata atcgccacgc cgacgtcttt aataatttcg cggaaacggt
    37741 tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc gtaccgccag
    37801 tatgaccgag gccgcgacca gagatcatcg gaatatagcc gccgcaggct gcgaccatcg
    37861 gccccaacat cagcgaagtc acatcgccga cgccaccggt ggagtgttta tcaacaatcg
    37921 ggccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc atcgccatgg
    37981 tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc gcgagggcgg
    38041 caatctgccc ttcggagata gtgttgtcgc gaataccgtt gataaagaaa cgaatttctt
    38101 catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg agaaacaagg
    38161 taacctcccg aggaaaagag tatgaggtgg cttttgccgg atgcgtctac gccttatccg
    38221 gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc gtaaagcatc
    38281 ttacttagta gctgctggcg ctcttaccgt cgccgtgacc cagcgctttc agcaggcttg
    38341 ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca gcaccgaaca
    38401 gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg ccgcccgccg
    38461 gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc atgatgcgcg
    38521 cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg aagtccgcac
    38581 ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc agttcgccgg
    38641 tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta caggctttca
    38701 ccaggtcaaa accaacctgc tcgttacccg ccatcagcgc gcggtacggg aacacaacgt
    38761 caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg atttcgatgt
    38821 cgtcgttacc gtgtgggaag ttggttaccg tagcaatacg gatttccggg gtgccctgct
    38881 ctttcagtgt tttgcgagca atcgggataa agcgaggata gatacagata gcggcggtat
    38941 tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg gtgtcgtcgt
    39001 cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct ttcagatcag
    39061 tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg ttagtattct
    39121 aacatctact ccgcaacaca cttcgatata catcacaatt aaggaaatct acttacaagt
    39181 ttgcatcact ataatgcgat ctggttcaaa taattcactt tcaaatgaat gcgtcagtgg
    39241 tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg cgcatcacct
    39301 tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca acaacgttgc
    39361 cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt tctggcactg
    39421 gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg cgcgctggat
    39481 gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg ctatgtctta
    39541 cccgattacg cccgttttct ggcgaacggt tccgaatggc tggagctgga aggcgcgaaa
    39601 gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc gtcggtcaca
    39661 tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt tataattcta
    39721 acacaagacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga cagaaccctg
    39781 ccagacgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac ccgcgcgatc
    39841 ttacgtgcag acgcagattt gaagcaggtt tcaccgaacc tgacctttat ctacgatcct
    39901 gaaatcaccc ctgatgacct gctgctggaa gtggcgaaga acatctgtga atgtagcaaa
    39961 ccgcacatcg ccaacggtcc ggtgcatgat aaaattttca caaaaggggg ctacgggatt
    40021 gtgagctgtt acaactcact gccgctggcg ggtggtggca gcacgctggt acgccttaac
    40081 ctgaaagcca ttgccgagcg cagcgaatcg ctggatgact tctttacgcg cactctaccg
    40141 cactactgcc agcagcagat cgccatcatc gatgcgcggt gtgaattcct ctatcaacaa
    40201 tcacacttct ttgagaatag cttcctggtg aaagaagggc tgattaaccc tgaacgtttt
    40261 gtgccaatgt ttggcatgta tgggctggcg gaagcggtta acttgctgtg tgaaaaagaa
    40321 gggattgccg cgcgctacgg taaagaagcc gccgcaaatg aagtaggtta tcgcatcagt
    40381 gcacagctgg cggagtttgt cgccaatacc cccgtgaaat atggctggca aaaacgcgcc
    40441 atgttacacg cacagtcggg gatcagttcc gatatcggca ccacgccggg cgcgcgtttg
    40501 ccgtatggcg atgagccaga tccgatcacc catctgcaaa ctgtcgcccg catcatgctt
    40561 attattattc tggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    40621 aggcgttggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    40681 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    40741 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    40801 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    40861 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    40921 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    40981 tgcaatgact gtggggagtg cgtgccacag tatccgcatc aggcgttgca gattgttgac
    41041 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagaggtgt
    41101 ccgcaacatg ccacgccaat ggcgcaatcc atgagcgtgg atgaggtgct tagccatgtc
    41161 cgcaaagcag tgctgtttat cgaagggata acggtgagtg gcggcgaagc cacgacccaa
    41221 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccacctc
    41281 acctgcctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attgctcccg
    41341 gtgtgcgacg gcgcaatgct cgatctcaaa gcgtggggga gcgaatgtca tcagcaatta
    41401 accggacgcg ataatcagca gattaagcgc agcatctgtt tgctggcaga gcgcggtaag
    41461 ctggcggaac tgcgtttgct ggtaatccct ggccaagtgg attatttgca acacatcgaa
    41521 gaactggcgg cgtttatcaa gggagtcggc gatgttccgg tacgcctgaa cgcgtttcat
    41581 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    41641 ccgttggctg atgcgttacg ggagcgcggg gtgagccggt taatatttcc ggcgctgtat
    41701 ttgtgacatg ccggatacga cctacggttc ggcacagact tgtaggcctg ataagacgcg
    41761 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    41821 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    41881 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    41941 cgcatacgtg ttattaagca acacttcggc aatctcatct tctggttccg ggcgcaactc
    42001 gcaaagcacg tcaaacaccc gcgcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    42061 gttgagcggc atatccggcg cgtcggtttc cagcaacaac gatgccagcg gtaattttgc
    42121 gatgacaacg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    42181 gtagcccagc tgtacaaacc gctcggcctg ttgcaggctg ccggaaaaac cgtgaaccac
    42241 gccagtgcgc gataaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gtgtgcgccg
    42301 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagaaa
    42361 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccgc tctcccccac
    42421 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgatcaa gagacacatc
    42481 gctatgtttt tccaacatac caggatgcaa gcccaatgcg gcatacagcg gttgataatt
    42541 ttccgctaac gcctgcacgc gggcaaaatt ttccgcctcg gttgccggaa caatgatctt
    42601 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    42661 gaaatcaaaa tggcagtggg tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    42721 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    42781 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    42841 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    42901 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    42961 aatggcttcg gcggataaat ttcgaatatc cgcagcttgc ccggtggttt ctcaataaat
    43021 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    43081 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    43141 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    43201 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    43261 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    43321 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    43381 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    43441 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    43501 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    43561 atatacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    43621 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    43681 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    43741 ttaccaagcg ttacaggtat tcgctgcccc acctctgcct tcctgttttt gttgtgaaag
    43801 aatgacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    43861 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    43921 actcaccagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    43981 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    44041 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    44101 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    44161 gggaggacga attacgacga attacttagt tttcagatca tttttaacgc ttttcacacc
    44221 atctaccgct ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacgatacc
    44281 agagagctga accacgccat cggtggtttc aactttcaca tgacgggaag ggacgatatc
    44341 gtccgccagc aatttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt
    44401 cacagagcct tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcaccccttc
    44461 aacacctttc gccactttca ctgcctcttc ggcctgggcc tggctttcaa cgaaaccgct
    44521 cagggtcacg actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg
    44581 atccaccagc gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac
    44641 tttattcata gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt
    44701 ttccgcgtag gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt
    44761 cgaaatcttc agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga
    44821 gcctggcaac acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca
    44881 atggtcgcca tcacaaaagc gatgagtgat gaataacgac cattacagcc tctgaatcag
    44941 ttattaatat cggtagaatg acaatcgacg gctttagata ctgatatcta cgcattgaac
    45001 ggtatttaac gccgtcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac
    45061 cgctcgggat atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc
    45121 ggctaataag gaagggaaat tgacaggacg cagcggctac cgcgccctgg aggcaagaat
    45181 taatgctcgc gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg
    45241 ttgaccatgc tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt
    45301 tcgttcttac gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca
    45361 gtggcaacgt tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct
    45421 accaccacat caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt
    45481 ggacggaaca cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt
    45541 ttttgcttca gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga
    45601 ataccggtga acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg
    45661 tggttgtgca ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc
    45721 gccataaagg tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagc
    45781 ttcatgcctt tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt
    45841 ttcgggtcca tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct
    45901 tctacggtac gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca
    45961 tccagcatat gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt
    46021 tcgcccgcaa ggaacagctc tttgtcgaac tcgttagacg cgcctttcac cagttccagt
    46081 tcgtcacgca gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc
    46141 agccctttaa caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag
    46201 gtttcatctt tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccag
    46261 gtgatcggtg cgcagccaat tttcagctcg ttctcaactt catcgagcaa ttccatcggg
    46321 tcgcggatat cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg
    46381 gtaacttcca tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc
    46441 aggcagcagt ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc
    46501 ggggtgtcga gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta
    46561 atggagatcc cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa
    46621 ccacggcctt ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg
    46681 gtgatggtag tcttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc
    46741 acctcttgca aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg
    46801 ccgcgcttgt tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat
    46861 cagtgtttac acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg
    46921 atgccttctg gttgctcgcg atggtgtgca ttcagccagc aggtcgcaag cccggcgttg
    46981 atgccaccga gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca
    47041 ggattgcccg cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca
    47101 acttcttcgg aaatcaccag caaatcgaag taatcacgca ggcccgtgcg ttccagacgc
    47161 acctgttgca aggcactaaa gccgttggtg atgatgccga ttttggcgtt gccacgaatg
    47221 gcgttaagca gagaaaccgc gcccggcagc ggcgtgcaga tttccgccat cgcattaata
    47281 aaggcttcgt tgagtttacc tggctcgacg ttcagccgtt cggcccagct ctcaaaccgc
    47341 ccgtgctgaa gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg
    47401 ttaacggcct gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac
    47461 atccgctgca ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa
    47521 atccagtccc acttcattat tccaccttgt cttacatact gattggcaac gccatgatga
    47581 tggcgtcttc gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa
    47641 agcctaaact ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata
    47701 gtgtcgccac gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gcccttccca
    47761 atccctgacg ctgatagtca ggatcgaccg caatattgaa caatgtagct tcatccagca
    47821 ccacttgcgt aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat
    47881 aacgctcgcc ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt
    47941 gttcaatgtg gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat
    48001 gtgcaaattt gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc
    48061 gccggtgatg ccacctgagc gccttccagt gatagcggtt cgtcagtacc caaccgccag
    48121 ctgttgcagc gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact
    48181 tgatcggggc tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg
    48241 ggaagatcgt ttgccaccat caccagacgg acgtgtgccg ggatcgcaat ggcaatctcc
    48301 ccctgcaacg cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc
    48361 cagtctcgtc gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa
    48421 ttcgtcgata ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt
    48481 ccatgtctgc atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac
    48541 aaagccgtat tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg
    48601 cggccagccg tgctcatacc cagcaattcc agcattggca ggtgttaagc cgccagatgg
    48661 gggataacgc ccgttttagt ctggtcgcca cggcggatga tgtcgcagat tgcgatacgc
    48721 tgatttacta ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt
    48781 ctctgcttcc ggtggggacg gatatttttg ttgttggcga gaaccgcagc ggcgtgcgca
    48841 gcgccgagca gatgctggct gattatgcgc cgttgaataa agtcgacagc gcccgtcgct
    48901 gtggcctcta ttttggtcgt ctggaaaaac agccggtatt tgatgccgat aaattctggg
    48961 gcgaatacag cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg
    49021 gtctggatgt cggtagccag ttgctgctct cgacgttaac cccgcacacg aaaggtaaag
    49081 tgctggatgt cggctgtggc gcgggcgtgc tttcggttgc ctttgcgcgc cattcgccga
    49141 aaattcgtct caccttgtgc gatgtctctg cgccagcggt agaagccagc cgcgcaacac
    49201 ttgcggccaa cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag
    49261 gtcgttttga tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg
    49321 atgcggcgca aacgctgatt cgcggtgcgg tgcgtcatct taatagcggc ggcgagctgc
    49381 gaattgtagc gaacgccttc ctgccttatc cggacgtgct ggatgagaca tttggcttcc
    49441 acgaagtgat cgcgcaaaca gggcgcttca aggtgtatcg cgccattatg acccgccagg
    49501 cgaagaaagg ttaattatct cgccgaatac cgtgtcggat gcggcgtgaa cgccttatcc
    49561 gacttacaaa acctgcacgt tagtctcctg atggacattt ttccagcaat tacacatcag
    49621 gcgataatta actattgacg aaaagctgaa aaccactaga atgcgcctcc gtggtagcaa
    49681 ttctttttaa gaattgatgg tatgcgaagg tggcggaatt ggtagacgcg ctagcttcag
    49741 gtgttagtgt ccttacggac gtgggggttc aagtcccccc cctcgcacca attatcttta
    49801 cttcctttct tgtttcttcc ctgttttttc atccctgatt caaattcatt atcatcagca
    49861 tgatgccagc aataagcgcc aggcacgatg gtagcaaaat gtaatgccgc aactgtttgt
    49921 gatacagcat caccggcgtc aatactaatc ccatcagaat gacaatccgc cactgatccg
    49981 gcgataaatc cgcatacatc agccctgcaa tcagaaatcc cggcagcaac actccccagc
    50041 cactgcccag cgtacgttgc aacatggttt tatctctttc attgataatg ataaccaata
    50101 tcatatgata atttttatca cttgcaagcc agagaaatcc cttgctatcg ggtaaaccta
    50161 tcgctaggat tagcaatcat tatcatttag attactatcc cgattatggc ctatcgttcc
    50221 gcaccgctct atgaagatat catctggcga acgcatctcc agccacagga tgctaggctt
    50281 gcacaagccg tacgtgcgac gatcgccgaa catcgtgaac atttgctgga gtttatccgc
    50341 ctggatgaac ccgctccgct taacgccatg acgctggcgc aatggtcatc accgaatgcg
    50401 ctaagttctc tactggcggt ctattccgat catatctatc gcaaccaacc aacgatgatc
    50461 cgcgagtaca aaccgctgat ctcactatgg gcacaatggt atatcggcct gatggtgcca
    50521 ccattaatgc tggcgctact gacgcaggaa aaagcattag atgtgtcgcc ggaacatttc
    50581 catgctgagt ttcacgaaac cggacgcgtc gcctgtttct gggtcgatgt gtgtgaagat
    50641 aaaaacgcaa caccacattc gccgcagcag cgaatggaaa cgttaatcag ccaggcgctg
    50701 gttccggttg tgcaggcact agaagcgacc ggagagatca acggcaaact tatctggagt
    50761 aataccggtt atttgatcaa ctggtatctt actgagatga agcaactgct cggcgaggcg
    50821 acggttgaat cgctgcgcca tgccctcttt tttgagaaaa cgcttactac cggtgaagat
    50881 aatccactct ggcgtaccgt ggtgctacgc gacggcctgc tggtgcgccg cacttgctgc
    50941 cagcgttatc gcttaccgga tgtacagcaa tgtggcgatt gtacgctgaa atgaaagcac
    51001 tgccggggaa gtaaacccgg catcatgcgg attaataggg atgcaacaca ttacttgttt
    51061 cgcaatgccg catacataac agaatatctg cggcctccaa caaccctgcg tcagaactga
    51121 cgcccagttt cgacatcaca ttaaatttat gtgcacggat cgttttgata ttgcgtttaa
    51181 gctgctcggc aatttgtgtc attgagtagc cacgcgacat aaagcgcaat atttctctct
    51241 ccgtcgggct taacgtccgg ctttggttaa tgtaccactg attgttcagt cggtcggtcg
    51301 cctgacgtac accatttaat gacaaaaaga gttcctgatg aaaaatctcc agcgttgacg
    51361 ctttacttaa tacaccgtcc agcggtgatg gcgacaatga accaatcagc cgagcttcaa
    51421 tatcatcatc cgcaataact aaacgccggg tacgcggaaa cttaatcgcc agttcagtca
    51481 ggcaagataa tccctctctg cgctcacttc tcatggcaga aaaagaaaaa atgaccgccg
    51541 caaacgagat ccgcgacatg gcatcctgaa atgactcctg actggtatag gtatggagct
    51601 tataagggtt gcccgcaaac tttctgaata aactctcaat accgatacta ctcatgacgc
    51661 atttttctat gagtgcgaca tttctcttct taattcggct gtgttccatt ctgcggcatc
    51721 tccataaaca gaaggcaagt taagttctct tgcaccctgc gcgctattta tccatgcata
    51781 catttcagcg ttgctgtgaa ttgccagtcg acgcatcgca ctgttttttt gggcgctgat
    51841 agttttatta cttttcttaa gcaatgaagc tatctggttg atgccccatc ctttacctaa
    51901 tagccgcagt accttgcgct ctgaaagagt gagaatgacg gagcgatagc taaaatcatg
    51961 agtttcagtc attgccggag aggtcagcat ggcgctgata cgctctgcgt gcgtattgcc
    52021 ggaacgtacg gtcttgacca gattctcaat gggttcaaca tcagacaata acgtcgccgt
    52081 agggcacatg agcagttcta cagcctggga ataccaagag cgtgacacca tgaaaaccca
    52141 gtgaatttcc cgatattgtg agattaaaga ataataatgt tcacaaacag aacgtggatc
    52201 ttcactttca ccggctaaat cagcaatgac taatccggaa cgacgtaatt gtaataaggt
    52261 aaggtcctcc gcagaggcgc tggatattat ttcatattca gggaagtgag tcctcatgac
    52321 ctcttttaac cctgcttgca taacaggtat tttactgata acaattccat ttttgcagca
    52381 tcctggcaac atatttcctc cacatccact gtctaaaaat tatgatggtt tattcaggat
    52441 attcattcct taaatgtttc cagcaaatta atagattaag cgaataaatt atccttagca
    52501 ttcactttca tatcagcata ctcctgctgc acaacaactc cactgcatgt gtcagaaggt
    52561 tagcatcaaa tgataacctg gtgaaatagc ctggagacat ttaaaatatc tacattaagt
    52621 tgttagaaat acttaatgaa aaacagaaat ctcattcatt taatttataa ttaaatgata
    52681 tctcttcccg cagaatgcgg gaatcacaca atcaatattt attcaataaa atctgatgat
    52741 tttaacttgt taacactaaa aaacgcgccc agctatctat tatcctgacc cgtaaaggct
    52801 aaaacaataa attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat
    52861 aattcgcttt ttatcaccga gcttgtcccg ataaccagga ttaatcagcg caattttgat
    52921 cttaacctac cgcaaaacaa ccttgcttca tgatacaaat ggattctcat tatctgaaca
    52981 atacgcagca cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa
    53041 cacggctttg tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg
    53101 ttgatgagct ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta
    53161 tctcttcgaa cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac
    53221 gtaaaaatca cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga
    53281 ttcttgcgga gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc
    53341 agccattacg ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt
    53401 tctgtaaact caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga
    53461 ccgcgatgta tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt
    53521 gccttaccgc tttcgccgcc accaccattt ccggattgct tttgcaacac cccactttca
    53581 gcaatacccc caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt
    53641 tgattaatgc cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg
    53701 cgatcgccag tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt
    53761 gggggctacg cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct
    53821 cgccgccatt cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg
    53881 ctggtgtgcg ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg
    53941 gttgaatatt gagtggtcaa cctttatggc ctccatgctg ggcggcacca ttggtattca
    54001 atggtcgcgc tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat
    54061 gttcccgggc atatcggctt ataccgcaat gatttcggcg gtaaaaatca gccagttagg
    54121 ttacagcgaa ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt
    54181 tggtgcgtta tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg
    54241 cgtataaaat tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt
    54301 tactataaaa gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt
    54361 ccagagtttt gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg
    54421 ttctggcaaa agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt
    54481 atgccgtcac gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctggcgcgtc
    54541 cgggaagcga tgtcgatctg gacgatcaac tctatcagga accgcaagcc gctcccgttg
    54601 ctgtacccat ggggaaattc gccatgtatc cggactggca acccgatgcc gactttatcc
    54661 gcctggcggc tctatggggc gtggcgctaa gagagccggt aaccaccgaa gaactggcct
    54721 cattcattgc ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa
    54781 aactggcgcg cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg
    54841 tgaatacggt cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa
    54901 aaacgttggc gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc
    54961 attcaaaacg ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc
    55021 cgctctcgaa cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat
    55081 tcgcccactg catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat
    55141 gaatgcgtta agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt
    55201 catcttttct ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga
    55261 gctgctgcta cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat
    55321 gaaagatacc ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa
    55381 cgttgatctg ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt
    55441 gatcatcaac cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac
    55501 caacagtaat atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg
    55561 cctgggtaac agtttgtggg tgatcttcaa ctgggatagc taccgtagcc gtgtaacagg
    55621 taaagagtat taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat
    55681 gatgatgaaa actgttaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc
    55741 caccggggtg catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg
    55801 caaccaaaac agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct
    55861 tagcagcgga aaccaggcct taagcgcaga taacatgaac aatgccgcag gcattctgca
    55921 atactgcgcg aagcaaaagc tggcttcggt aaccgatgtc gaaaacatca agaaccaggt
    55981 gctggaaaag ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg
    56041 cattcagggt ttgctgaaaa ccaaagatgg tcagcaactc aatctggata acatcggaac
    56101 aactccgctg gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct
    56161 gaacttcatt tcctgaaccg cgaaacgccg cgttttagct gttacccggc tgacgcggct
    56221 ctctcctgcc cttttccctg ctcctggacg gttttccccc tgttacacgg gaagcgaccg
    56281 cgatcaaaaa agtctgttta taaactaatt cctaacaaca tcgcattttt ttgacactag
    56341 aattgcagca atatggcgac gccattacat tgtgcttctc aaaaagctta aacgttggct
    56401 aaacggcccg gtttagtgag gatcgcccgt tgtcagaact actctctttc gccctttttc
    56461 tcgcctctgt gctgatttac gcatggaaag cgggacgtaa cacctggtgg tttgcagcca
    56521 cattaacggt gctggggcta tttgtcgttt taaatatcac cctgtttgcc agcgactatt
    56581 ttactggcga tggtattaac gacgcggttc tctatacctt aaccaacagc ctgaccggtg
    56641 ctggcgtcag caaatacatt ttgccgggta tcggcattgt gctggggctg acagcggtgt
    56701 tcggtgcgct gggctggatc ctgcgccgtc gtcgccatca tccgcaccat tttggttaca
    56761 gcctgctggc gctcttactg gcgctgggtt cggtggacgc cagcccggca tttcgtcaga
    56821 taacggaact ggtgaaatcc cagtcacgcg acggcgaccc ggactttgcg gcttattata
    56881 aagagccgtc gaaaactatc cctgacccga aactcaacct ggtttatatc tacggcgaaa
    56941 gtctcgagcg gacctatttt gataacgagg ctttcccgga cctcacgcct gaactgggcg
    57001 cgttgaaaaa tgaaggcctg gatttcagcc acacgcagca gctgccggga acagattaca
    57061 cgattgcggg catggtggct tctcagtgcg gcataccgct gtttgccccc tttgaaggca
    57121 acgcctccgc ttctgtttcc agcttcttcc cgcagaacat ctgtctgggc gatatcctga
    57181 aaaactcggg ttatcagaac tatttcgtgc agggcgcgaa tctgcgtttt gccggtaaag
    57241 atgtgttcct gaagtcgcat ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg
    57301 tggtagccga cccgcactat cgcaacgact ggggattcta cgacgatacc gttctcgatg
    57361 aagcgtggaa aaagtttgaa gagctttccc gctcaggtca gcgattctca ctgtttaccc
    57421 tgacagtcga tacccatcac ccggatggtt ttatctctcg tacctgtaac cgcaaaaaat
    57481 atgattttga cggtaaaccg aatcagtcat tcagcgcggt aagttgcagc caggagaata
    57541 tcgcgacgtt tatcaacaaa atcaaagcgt caccgtggtt taaagatact gtcatcgtcg
    57601 tctcttctga ccatttagcg atgaacaaca cggcgtggaa atacctcaat aagcaggatc
    57661 gcaataacct gttttttgtc attcgtggcg acaagccgca gcaagagacg ctggcagtga
    57721 agcgtaacac gatggataac ggagcgacag tgctggacat tctcggtggc gataactatc
    57781 tcggacttgg tcgtagcagt ttatccgggc agtcaatgtc ggaaatcttc ctcaatatca
    57841 aagagaaaac attggcgtgg aagccggata tcatccgtct gtggaaattc cctaaagaga
    57901 tgaaagagtt caccatcgac cagcagaaaa acatgattgc cttctcgggt agccatttcc
    57961 gtttgccgct gctgttgcgg gtttcagaca aacgcgtgga accgctgcca gaaagcgaat
    58021 actcagcacc gctgcgtttc cagctggccg atttcgctcc acgcgacaat ttcgtctggg
    58081 ttgaccgttg ctacaagatg gcacaactct gggctccgga actggcactt tccaccgact
    58141 ggtgtgtctc gcaggggcaa cttggcggtc agcaaattgt tcagcatgtt gacaaaacaa
    58201 catggaagag caagacggca tttaaagata cggtcatcga catggcgcgt tacaaaggca
    58261 atgtcgatac gctgaagatt gttgataacg atattcgcta caaagccgac agtttcatct
    58321 ttaacgtcgc cggtgcgcca gaagaggtga aacagtttag cgggatttcc cgtccggagt
    58381 cgtggggccg ctggtccaac gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc
    58441 cgctgccgaa gaaatttgac ctggtgatta ccgccaaagc atacggcaat aacgccagcc
    58501 gtcctattcc ggtacgcgta ggcaatgaag aacaaactct tgtgctgggc aatgaagtga
    58561 ccaccaccac actgcatttc gataacccaa ccgatgccga cacactggta attgtgccgc
    58621 cggaacctgt ctcaaccaac gaagggaata tcctcggaca ctcgccgcgt aagctcggga
    58681 tcggcatggt ggaaattaaa gtggtagaac gtgaagggta attcctgact gatgtgtagg
    58741 tcgcataggg tgcgacctac acaccttctc cggcttacaa cacattatta agaaacaaaa
    58801 aaaacagcgc ccaacacttc cccggacgct gcatggcaac tttaataatc tcgcctttca
    58861 gaaagtaatt acgcctttaa ttaactcacg attgttaatc acatcgcgct cgtaggtttc
    58921 tgccagcgtg gcgaacggat agcgatgggt taacatcatg ccagcagtga tttttccttc
    58981 cgccatcagt cgaccgactt tagcaaaatc ttccggcgtg gcgttgcggc tgcccatcat
    59041 cgtcgtttct tttttatgga attccggatc ggagaactgc aactcacctt taaacaggcc
    59101 gacaaatacc accgtgccgc cgtgacgaat cagattcacg gtgttattca tcgcatgttg
    59161 attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgcagctg
    59221 cgcgtcaaaa tcttcggctg acgggtccag cacgggcaac tccagacgcg ttgccacatg
    59281 ttcacggcgc gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat
    59341 tgccgccgcg cccagaccga ttggcccggc cccgaccacc agcacctgct cgccgggagc
    59401 aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgcggccgc
    59461 ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact
    59521 aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat tggtacgccc
    59581 gcttttacac gccgggcatt gctgacaggc aacataaggg atcacagcaa cttgctgacc
    59641 atttttaaga tcagcaatat ttttacccag cccaacaatc tccccacata tttcatggcc
    59701 taaaacccgt ggataactaa aaaatggttg atttccaccc caggcatgaa tatcggtacc
    59761 gcaaatcccg acagacttaa tttttattaa tgcttcattg tcacccggaa taggtatctc
    59821 gcgttgtttc cagactaatt ctttcggctg ctggcaaatt aaaacattca tcgtagacat
    59881 gattcggctc tccatttttt gttgccttag atatcaatga aaatgaaaaa tggcagcgga
    59941 acggaaaatc tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg
    60001 actcacaagg aggaaaatat gagtcgttca caaaatttac gccacaatgt gattaaccag
    60061 gtcattgatg atatggcgcg gggtcatatc ccttccccac ttccatcgca aagtgcgctg
    60121 gcggaaatgt acaacatcag ccgcactaca gtgcgtcata ttctcagcca cttacgcgaa
    60181 tgcggcgtct taacgcaggt tggaaacgac tatgttattg cgcgtaaacc tgaccatgat
    60241 gacggtttcg cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct
    60301 tttttcacga tgataaacca gcgccagtta cgcccagggg aaactttttc tgaactgcaa
    60361 ctggctcggg ccgcgggtgt cagcccggtg gttgtaagag aatatctttt aaaattcggc
    60421 cgttataatc ttattcaaag cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa
    60481 tcctacgctg agcagctgtt tgagctacgc gaaatgctcg aaacacattc acttcagcac
    60541 tttctcaacc tgcctgatca tgatccccgc tggctgcagg cgaaaaccat gctcgaacgt
    60601 catcggctac tgcgtgacaa catcggcaat agctttcgca tgttctcgca acttgacaga
    60661 gattttcact ccttactgct ttcagctgcc gacaatatct tttttgatca atcgcttgag
    60721 atcatctcag tcatctttca ttttcactat caatgggacg aaagcgatct taaacagcgc
    60781 aatatcattg ccgtcgatga gcatatgacc attctcagcg ctctgatttg ccgcagtgac
    60841 cttgatgcca cccttgcgct ccgtaaccat ttgaattcag cgaaacaatc aatgattcga
    60901 tccattaatg agaacacgcg ttatgctcat taagtacgga ttaaaaacag caaactatta
    60961 gcgggtgcac ggttttatca aaagataaat accgggaaat tcccgcttac ctatgctcac
    61021 aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc
    61081 gttaatgtca accttgcgta atgcacaatt acgtaaacaa ttaaaattat aaatacagga
    61141 gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt tcttcattta ctccatcttc
    61201 gtcggcagat attcccgtgc caccagatgg attagttcaa cgcagtaccc gaattaaacg
    61261 cattcaaacc accgccatgt tgttattatt ttttgcggcg gtaatcaatt atctcgaccg
    61321 cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa gtgccaccga
    61381 aatcggcgct ttgctctccg tgttttcact cgcttacggc attgcgcaac ttccttgcgg
    61441 cccactattg gatcgtaaag gcccacgcct gatgctggga ctggggatgt tcttctggtc
    61501 actgttccag gccatgtctg gcatggtgca cagctttacg cagttcgtgt tggtgcgtat
    61561 cggtatgggg attggtgaag cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg
    61621 gttcaacatc aaagagcgcg gacgcccgat gggcttcttc aacgcagctt ctaccattgg
    61681 cgttgccgta agcccaccga ttctggcggc gatgatgctg gtgatgggct ggcgcgggat
    61741 gtttattacc attggtgtac tggggatttt tctcgccatc ggctggtata tgctctatcg
    61801 caaccgcgag cacgtagaac tgactgccgt tgaacaagct tatctcaatg caggtagcgt
    61861 caatgcccgc cgagatccgc tcagttttgc cgaatggcgc agcctgttcc gtaaccgcac
    61921 aatgtgggga atgatgctcg gattcagtgg catcaactac actgcgtggc tgtatctggc
    61981 ctggcttcct ggttacctgc aaacagccta taacctggat ttaaaaagca cagggttgat
    62041 ggcggctatc cctttcctgt ttggtgctgc cgggatgctg gtcaacggtt acgttactga
    62101 ctggctggtc aaagggggaa tggctccgat taaaagccgt aagatctgca ttattgccgg
    62161 gatgttctgt tctgccgcct ttacgctgat agtaccacaa gcgacaacat ccatgatggc
    62221 ggttctgctg attggcatgg cactgttctg tattcacttt gccggaacat cctgctgggg
    62281 cttgatccac gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta tccagaactt
    62341 tgccagcttc atctgcgcct ctttcgcgcc gatcattact ggttttattg ttgatacaac
    62401 ccactcattc cgtctggcac taatcatttg cggttgcgtc accgcagcgg gagcactggc
    62461 gtacatcttc ctggttcgtc agccgatcaa cgacccgcgt aaagattaaa ctccctatgc
    62521 ccgataacat tttgcttatc gggcattttt atggcgatta aaacgtttcc cagttctccc
    62581 cgctatctgc cactgccatt ttacgcggcg tagctggcgt cacggttttt accacagccg
    62641 atgtttcacg ctgctgttgc tgctgttgct gaatccggaa cactgccaca gcttcggtca
    62701 ggcgactggc ctgctcttcc aacgcggcgg cggcagcggc agactcttcc accagcgcag
    62761 cgttctgttg agttacccgg tccatctcag caaccgctaa gccaacctga tcgataccac
    62821 ggctctgctc atcagaagca gaagctattt cgcccataat gtccgtcacg cgggtcacag
    62881 cactgacaat ctccgccatt gtttccccgg cgctttcgac cagcgtagag ccaacatcca
    62941 ctttccccac cgagtcttca atcaggcttt taatttcacg agccgcctgg gcgctacgct
    63001 gggccagatt acgcacttct cccgcaacca ccgcaaaacc acgtccttgc tcacccgcac
    63061 gcgctgcttc aaccgccgcg ttcaaagcca gaatattggt ctggaaggca atgccgtcaa
    63121 ttacgctgat aatatcggcg attttctgcg aactggtgga gatatcacgc atagtctgca
    63181 ccacgttatc taccactttg ccgccgcgtt gcgccgtttc agaagcactt aacgccaggt
    63241 ggctggcctg gcgcgcattc tcggcgttct gtttcaccgt tgcggtgagt tgctccatgc
    63301 tggctgccgt ctcttccagc gaagcggcct gttgctcggt gcgcgaagag agatcgttat
    63361 tgccggtagc gatttcgctg gcaccgctat agatggcatt ggccccgttg cgcacatcac
    63421 cgacggtacg catcagctct ccctgcatat ggcgcaaact ctctgccagt tgccccatct
    63481 cattagagcc atccacctca atcggtttca ccagatcgcc gcctgcaata tgacgaatgc
    63541 tgtcaatcag gcgattcatt ggcgctacca gcgaggcttt aataccgaac cagacggcga
    63601 agatgaccgc cagtacgacg atcatcacgc ccaccagaat ccacatcgcc tggctgtagg
    63661 aggcattgtt atcgctgacg gcgatatcat agagccgatc gttttgctcc atgtaagcca
    63721 catactgctt ctcgaaaccg tcctgatatc cctgggtcgg ctgatcaaag aactcgttga
    63781 ttttgcctgc acctaacagt tggatcagct ccgccagcgc attgtgataa atatcgtaat
    63841 tacgtttgat ctctgccgct gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt
    63901 aatctgccca gtttttttcc gcctgtttca acgaaatact ggcactctgc atcagctcag
    63961 caacggttga accgctacca atattattct gatccatcat gtagcggata cccgcgcggt
    64021 tgagggtgtt acgcgtctgc aacaacgcga cccagctgcc attcagcgtg gattgctgct
    64081 ggcgaatggt ttgtaaaaca gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca
    64141 gaccgcctga tgtcagttgt aaaaggccaa aaacggccaa aaccagcagt aagctggtca
    64201 cgatcttgat acgttttaac atggtttctc tttcctgtgg accgatgcaa aattttcggc
    64261 ctgaaatgag aaaactttat aggtagcgac ttgaaactgg ttaattttac ctcacttttt
    64321 ttaatttttg ttcaaaaata caataaccac ttattgatac attttctttt cttatttttt
    64381 tcacacgaat tgtatctatt ttgaacgaaa tattttatag agtgtggaat tttaagaaag
    64441 gacatctgaa atgttaaacc gtgacttcga tcatctctca acgctattac atctcttttt
    64501 tgaacgtgag gatatttgcc aaaagcttaa tattattgac caaaacaata tcaccggttg
    64561 ggaagtgtgg ttccaggtag agtttgctaa tatgctctgc tcaacagacc atgaatggtg
    64621 gcgtgaacaa gcactttcct gtgatatgcg caaaaaacct gagcgcccga cactacgcac
    64681 agatttctta ctacgtaaaa aaggctgggc acaagatagc tatatcgcgc ttgaaataaa
    64741 acaaaacaga gacgcgacca gttgtgttaa aaatatgatc gcagacttag aaaaaagcgc
    64801 aaaaataaaa cgttccgaac tcgatttacg cagtttctgg accttgggta tcacccctat
    64861 gatagacagg gaaaggctgg actctctcat cgattgctat ttagatgaga aattttatct
    64921 tactaaatct cgtaaaaaac acgttttact taaagaaata aataacacac catattgcta
    64981 tattgttatt taatatttat agtatgaata taatttttaa atgagttaca caaccgaggg
    65041 ctaaaaatcg cttcggttgt gatcatcatc acataaataa ccttcataaa ccttgctcaa
    65101 aaccgctcaa ccgcaaaact gaccgcttac gtccctaaaa taaccactca gttatttacc
    65161 ttactttacg cgcgcgtaac tctggcagca tcattgcagg atagcggtca atttacctcc
    65221 tcaaacgcaa cgcaaaccta gaacggcttc ggccaactat taatcaatac atgccaggtt
    65281 ttactatgga tacgaaaaaa ctattcaagc acataccctg ggtgattctc ggaatcatcg
    65341 gtgcattctg cctcgcggta gttgcattac gtcgggggga gcacgtcagc gccctgtgga
    65401 tcgtggtcgc ctctgtgtcg gtgtatctgg tggcgtatcg ctactacagt ctgtacatcg
    65461 cccagaaggt gatgaaactc gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg
    65521 gtctgaacta cgttccgacc aaccgttacg tgttgtttgg tcaccacttc gccgctatcg
    65581 ccggtgctgg tccgctagtg ggaccggttc tcgccgcgca gatgggctac ctgcctggca
    65641 cgctgtggct gctggcgggg gtagtgctgg ccggtgcggt tcaggacttt atggtgctgt
    65701 ttatctcctc tcgccgtaac ggcgcatctc tcggtgagat gatcaaagaa gagatggggc
    65761 cagtaccggg gactatcgcg ctgtttggct gtttcttaat catgatcatc atcctcgccg
    65821 tcctggcgct gattgtggtt aaagccctgg cagaaagtcc gtggggtgtc ttcaccgttt
    65881 gctcaaccgt accaattgcg ctgtttatgg gtatctacat gcgctttatc cgtccggggc
    65941 gtgtgggtga agtctctgtt attggtatcg tgctgctggt tgcctctatc tacttcggtg
    66001 gcgtgattgc tcacgatccg tactggggtc cggcactgac ctttaaagac accaccatta
    66061 ccttcgcgct gattggctat gcgtttgttt ccgcgctgct gccggtgtgg ctgatcctcg
    66121 caccacgcga ctatctggca accttcctga agatcggcgt tatcgtcggc ctggcgctgg
    66181 gtatcgtggt gctgaacccg gaactgaaaa tgcctgcgat gacccagtac attgacggta
    66241 ctggcccgct gtggaaaggc gctctgttcc cgttcctgtt catcaccatc gcctgtggtg
    66301 cggtatctgg cttccacgcg ctgatctctt ccggtacgac gccgaaactg ctggctaacg
    66361 aaaccgacgc gcgtttcatc ggctacggcg caatgctgat ggagtccttc gtggcgatta
    66421 tggcgctggt tgctgcatcc atcatcgaac cgggtcttta cttcgcgatg aacaccccgc
    66481 ccgcgggcct tggcatcacc atgcctaacc tgcatgaaat gggtggcgag aacgcgccga
    66541 tcatcatggc gcagctgaaa gacgttaccg cacacgcggc agcgaccgtc agctcttggg
    66601 gcttcgtgat ttcgccagag cagatcctgc aaaccgcgaa agacattggt gagccttctg
    66661 tcctgaaccg tgcaggtggt gcgccgacgc tggcggtagg tatcgctcat gtgttccaca
    66721 aagtgctgcc gatggctgac atgggcttct ggtatcactt cggtattctg ttcgaagccc
    66781 tgttcatcct gaccgcgctg gatgcgggta cccgttctgg ccgctttatg ctgcaagacc
    66841 tgctgggtaa cttcatcccg ttcctgaaaa aaaccgactc tctggttgct ggtatcatcg
    66901 gtactgcggg ctgtgtgggt ctgtggggct acctgctgta tcagggcgtg gtcgatccgc
    66961 tgggcggcgt taagagcctg tggccgctgt tcggtatctc caaccagatg ctggcagccg
    67021 tagcgctggt actgggcacc gttgtgctga ttaagatgaa gcgcacccaa tacatctggg
    67081 taactgttgt tccggctgta tggctgctta tctgcaccac ctgggcgctg ggtctgaaac
    67141 tgttcagcac caacccgcag atggaaggct tcttctacat ggcaagccag tacaaagaga
    67201 agattgctaa cggtactgac cttacggcgc agcagattgc caacatgaac cacatcgttg
    67261 tgaacaacta caccaacgca ggcctgagta ttctgttcct gattgtggtg tacagcatca
    67321 tcttctacgg tttcaaaacc tggcttgcgg tacgtaacag cgacaaacgt actgacaaag
    67381 aaacaccgta cgttccaatc ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt
    67441 gtttagcccc gcttcggcgg ggctttgttc ttatcagagt gaactatgtt tggtaactta
    67501 ggacaggcaa aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactac
    67561 gacaactacg tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa
    67621 gaattcttcc gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc
    67681 tgttgttaat ggagagagca tgaacccgat tgcagttacc ctactcaccg gttttttagg
    67741 cgcgggcaaa accaccctgc tgcgccatat tcttaacgaa caacacggct acaagattgc
    67801 cgtgattgaa aacgaatttg gcgaagtctc cgttgatgat caactgattg gcgaccgcgc
    67861 cacgcagatc aaaacgctga ccaacggctg catctgctgt tcgcgctcca acgagctgga
    67921 agacgcacta ctcgacctgc tggataatct cgataagggc aatattcagt tcgatcgtct
    67981 ggtcattgaa tgcaccggca tggccgatcc cggcccgatt attcagacct ttttctccca
    68041 tgagatttta tgccagcgtt acctgctgga cggcgtgatt gcgctggtgg atgcggtaca
    68101 tgccgatgag cagatgaacc agttcaccat cgcccagtcc caggttggct acgccgaccg
    68161 tattctgctg accaaaaccg acgtcgcagg cgaagcagaa aaactgcgcg aacgcctggc
    68221 gcgcatcaac gcccgtgcac cggtctacac cgttacccac ggcgacatcg acctgggcct
    68281 gctgttcaac accaacggtt ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt
    68341 ccactttatc gcggataaac aaaacgatat ttcgtcgatt gtggtagaac tggattaccc
    68401 ggtagatatc agcgaagttt cccgcgtgat ggaaaacctg ctgctggaat cggcggataa
    68461 actgctgcgt tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca
    68521 gggcgtccag cgcctctaca gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca
    68581 tagcacaatg gtttttatcg ggattcagtt gccggaggaa gagattcggg cagcgtttgc
    68641 ggggttgagg aagtaagttc gggaaagatc aactttttga gagttccacc gcaacattat
    68701 tacgagtgga actctttaga tatgtcagac caaccaaagc ttcttcaatt gcgttttcat
    68761 tttttcatgt ctcattttca gttcagccaa tccccattct gaagaattac aaacatcttt
    68821 tgttaaatca aaagagcttt tactattaag tttctcataa atagatttct ttttgataaa
    68881 atcatagtgt tgtgcttcag aatttttgga accacttatg agtgttaaat tacctatttt
    68941 atgcagccag tagacatgct cttgaggttg gaatctatta atccagtatt cattaactaa
    69001 tgcttgagga agaatatgct caattgtgat tctccccgta taggttttaa tcacgctctc
    69061 atcttgttgc tccatatcta agcgaagcaa aattgctttt attaagttca cttgattcgg
    69121 acgaggctca tataaatctt catccagagc agctataaaa cctgaattat ctgcatgttg
    69181 gtttatctga tttatcacag aatcaaaagg catatcatta tttattgcta ccagagctga
    69241 ataacaaacc atttctcttt ggcttttaat ttgttttttt aaccacccat gcatatatac
    69301 cttctcaaag gcggtaataa attgagaaaa atcatctaaa ttaaaatctt ctgtacgagc
    69361 cattcgattc atgaatgcca taaccggagg aatccactca tctaccccta aattacttag
    69421 cgatgctatt tttcgtcgta ttgaagggtg ttcaaaatca ttctctaaaa tcttagtata
    69481 attttttgca gaattaacca acatcagtga catagcaata gcatctccat caaattgctg
    69541 ctggagattt tcaataaaag cttcaaaacc tttttgtaac accctgtcac gatctttttt
    69601 ctcggaaagc ttatgaagag taagaaattt atcaagccgt ctcactccga ccatatcttc
    69661 aatttgcgac catgctgatt ctatttgctc tgatttttta ttatgtgtag aagctgattc
    69721 aaacagtgca ttttttaaaa gatctgcatt acttaaaggt aaacctcgac tatttaatac
    69781 attaaagagt ctaaatgatg atgcgaaatc atctgtttgc acaaatacga tatatacatt
    69841 ttgcaaaata tatttagcaa gcaacttcaa ctcatcaaca gataaccgca acaaataatc
    69901 tctaattgtt tcagcgttgc taatgaatac taactcagta tccgatggct tttcaggttt
    69961 ataatcttta gaatcctgca gtatataata tttataaaga tcatgctctt tttttctaac
    70021 aattagtctt ggttcatctg tttcatcaga ataaacatca ataggcagaa ctcttttttg
    70081 caaatcatct tttatcccct gaatcgggat gattttttta gctcggagac aataatgctt
    70141 aacgttgtaa gacgttgttg tccatctaca acttcatact gattttgccc tttattgata
    70201 caaatcatac tcccaataaa atatccattc tcttctgatt gagagctttt ataaatgtca
    70261 tcaatgagtt gctctgcatt atctaccgtc caactgtaag gacgctgata agaaggaata
    70321 ataaactttt tgttttcagt aaggatatcc tgcaccttac attctttagc ttccatttca
    70381 tatacccaat caaacaatca acaataacat tatctttctt gctcgaaaga aacgcaacta
    70441 taagattttt atatattttg ttagagttga tatggttcct gtaacttctg tttatctgtc
    70501 tctgatatgc ccagattaat tcaagcaaga tataacagag atatcaatgt gatctctgct
    70561 acgtacttta cgttgaaaca ccgccctaaa tcatagccag gctattgatt ttatgtacaa
    70621 tcagaatcgt cctgataaaa gcgagaatac cccatggcgg aactgaacct aagtaacctg
    70681 acggaagcag acatcattac caaatgcgtt atgccagcca ttctcaatgc aggctgggac
    70741 aacacaacgc aaatcagaca ggaggtcaaa ctccgggacg gtaaagtcat cgtacgtggt
    70801 aaagttgcgg cacgcagaac ggtaaaatct gctgacatcg tgctgtatca caaacctggc
    70861 attcccttag ccgtgattga agcaaaagcc aacaaacatg aaattggcaa agggatgcaa
    70921 cagggcattg aatatgcgcg cctgctggac gttccttttg tttttgccac caacggtgat
    70981 ggctttatct tccgcgatgc caccgcagcc gaaggtgaat gccttgaaaa gcaaataacg
    71041 ctggatgact tcccctcccc cgctgaactc tggcaaaaat tctgcctctg gaaaggatac
    71101 acacaagctc agcttccggt gattactcag gactattacg acgatggcag cggtaaatcg
    71161 ccacgttatt accagcttca ggcaatcaac aaaaccattg aagccgtctc caacgggcaa
    71221 aaccgcgttc tgctggtcat ggcgaccgga acagggaaaa cctataccgc attccagatc
    71281 atctggcgcc tgtggaaatc aaaaaataaa aaacgcattt tgttccttgc cgatcgcaat
    71341 attctggtcg accaaaccaa aaataatgat ttccagccat ttggtacggc aatgaccaaa
    71401 gtcagcggac gcaccattga tcccgcttat gaaattcacc tcgcgctcta tcaggctata
    71461 actggcccgg aggaagacca aaaagcgttt aaacaagtcg caccagattt cttcgatctg
    71521 atcgtgatcg acgaatgcca tcgcggcagc gcatccgaag acagcgcctg gcgagaaatc
    71581 cttgattatt ttagttccgc cacccaaatt ggcttaaccg ccacgccaaa agagacgcat
    71641 gaagtctcca gcacggatta cttcggcgat ccggtttacg tctactcact aaaagaaggg
    71701 atcgaagacg gcttcctcgc cccttataaa gttgtccgtg ttgatattga tgttgatctg
    71761 caaggctggc gcccaaccaa agggcaaact gacttaaacg gcgaagtgat cgacgatcgt
    71821 atctataacc agaaagattt cgatcgcacg atggtaatcg acgaacgcac agaactggtt
    71881 gccagaacca ttaccgacta cctcaagcgt accaatccga tggataaaac catcgtcttc
    71941 tgtaacgaca tcgatcatgc agaacgtatg cgccgcgccc tggttaatct caacccggag
    72001 caggtgaaaa agaacgacaa atacgtcatg aaaatcaccg gcgatgatga aattggcaaa
    72061 gctcagttgg ataacttcat caacccgaaa aaaccgtacc cggttatcgc gaccacttca
    72121 gagctgatga ccaccggtgt ggatgctaaa acctgcaaac tggtagtact ggaccagaac
    72181 atccagtcga tgaccaaatt caagcagatt atcggtcgtg gtacacgcat cgacgaacgt
    72241 tacggcaaac tctggtttac catcctcgac tttaaaaaag ccaccgaact gtttgccgat
    72301 gagcgtttcg atggcattcc cgaaaaagtc atggatacca caccagagga tatcgccgat
    72361 ccagaatctg attttgaaga gaaactcgaa gaaatcagcg aacatgacga cgaacaggta
    72421 acaggcgttg atgaaccgcc tgcgccacca taccaggtta aagataccga tgatgtcggc
    72481 ccacttccgg aagaagacga gaagaaaatc cgcaagtttc acgtcaacgg tgtagcagtg
    72541 ggcgttattg cccagcgtgt tcagtattac gacgccgacg gtaaactggt taccgaatcc
    72601 tttaaagatt acacccgcaa aacactgctc aaagaatatg cctcgctgga tgactttacc
    72661 cgcaagtggc aggacgccga tcgcaaagaa gcgatcattc acgagctgga gcaacagggg
    72721 atcatctggg aagtactggc agaagaagtc ggtaaagatc tcgacccgtt cgacatgctt
    72781 tgccacgtag tgtatggtca gccgccgtta acccgcaaag agcgcgccga gaacgtgcgc
    72841 aagcggaact acttcacaaa atactctgaa gcagcgcaag ccgtgctcga taatctgctg
    72901 gataaatacg ccgatgcggg cgtacaggag atcgaaagta ttcaggtgct gaaacttaag
    72961 ccattcgaca gcatgggcac cttaccggag attattaaaa ccggatttgg cgaccgtaac
    73021 gggtataatc aggcgctcag cgagctggaa aacgaaatct accaattacc gccccgctct
    73081 gcttaacaac tctcttttgc gccttctgca accatcatgt tgcagaaggc cttgtttttc
    73141 atggaataca acatgtctat cagctcagta atcaaatcat tacaagatat tatgcgcaaa
    73201 gatgccggtg tggatggcga tgcgcagcgt ctcggtcagc tctcctggct gctgtttttg
    73261 aaaatcttcg atgcccagga agaggcgctg gaactggagc aggataacta tcaatatccg
    73321 atcccacagc gttatttatg gcgcagttgg gccgccaacg cgcagggcat taccggtgat
    73381 tccctgctgg aattcgttaa tgatgatctg ttcccggcgt tgaaaaacct cactgcgcct
    73441 atcgataaaa acccacgcgg ctacgtggtg aagcaggcgt tcagcgatgc ctataactat
    73501 atgaaaaacg gtacgctact gcgtcaggtg atcaataagc tgaacgaaat tgactttacc
    73561 agcgccagcg aacgccatct gtttggtgat atttacgaac agatccttaa agatctgcaa
    73621 tctgcgggca atgcgggcga attctatact ccacgcgccg tcactcgctt tatggtggat
    73681 cgcgttgatc cgaaactcgg cgaatccatt atggacccgg cctgcggtac gggcggtttt
    73741 cttgcctgcg catttgatca tgtaaagaac aaatacgtga agagcgtcgc cgatcatcag
    73801 acgctgcaac aacagatcca cggtgttgag aaaaaacagc ttccgcacct gctggcgacc
    73861 accaatatgc tgctacacgg cattgaagtg ccagtgcaaa ttcgtcacga caacactctg
    73921 aacaaaccgc tttcctcccg ggatgagcaa ctggatgtca ttgttaccaa cccgccgttt
    73981 ggtggcacgg aagaagacgg tattgagaag aactttccgg cagagatgca aacccgcgaa
    74041 acggcggatt tgttcctgca actgattgtg gaagtactgg cgaaaaatgg tcgcgcggcg
    74101 gtggtattgc cggatggcac actatttggc gaaggcgtta aaaccaaaat caaaaagcta
    74161 cttaccgaag agtgcaacct gcataccatc gtgcgcttac cgaatggtgt gtttaacccc
    74221 tataccggca ttaaaaccaa cctgttgttc tttaccaaag gtcagccaac caaagagatt
    74281 tggttctatg agcatccgta tccggcgggc gtaaaaaact acagcaaaac taagccgatg
    74341 aagtttgaag agtttcaggc ggagatcgac tggtggggta acgaggccga tggttttgcc
    74401 agccgcgtag agaatgagca ggcgtggaaa gtcagcattg atgacgtgat tgcgcgtaac
    74461 ttcaatctgg atattaaaaa cccacatcag gcggaaaccg tcagccatga tccggccgag
    74521 ctgttagcgc agtatgcaaa acagcaggcg gagatccaga cgctgcgtaa tcaactgcgc
    74581 gatattcttg gcgctgcgct gtctggcaag gaggttaact aatgagtgtg gaaaagctga
    74641 tcgttgatca tatggaaacc tggacctcgg cgttgcaaac ccgttccacc gccgggcgcg
    74701 gcagttccgg aaaaattgat ttatatggca ttaagaaatt acgtgagctg attctggaac
    74761 tggctgtgcg cggtaaactg gtgccgcagg acccgaacga tgaaccggcg tcggagctgc
    74821 tgaagcgtat tgcggcagaa aaagcagagc tggtgaagca aggtaaaatt aaaaagcaaa
    74881 aaccactgcc ggaaattagt gaggaagaga agccgtttga attgccggag ggatgggagt
    74941 gggtgcgtat tagtgaaatt ggacatgact ggggacaaaa aactccagat aaagatttta
    75001 cttacattga tgttggttca ataaataaag aatatggaat tattgaagag ctatctattc
    75061 tttcagcgaa agatgcccca tcgcgagcga gaaaaattgt tcagcaaggg actatcatat
    75121 actctactgt tcgcccgtat ttgttaaata tagctattat tgaaaacgaa atactacctg
    75181 aaccgatcgc tagtactgcc ttcgctatta ttcatccata tacggcgatg gatgctaatt
    75241 tcatttatta t
//
LOCUS       DABSYT020000014        65323 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.014, whole genome shotgun sequence.
ACCESSION   DABSYT020000014 DABSYT020000000
VERSION     DABSYT020000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.014"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(20..502)
                     /gene="folK"
                     /locus_tag="ID725_RS06270"
                     /old_locus_tag="pgaptmp_001254"
     CDS             complement(20..502)
                     /gene="folK"
                     /locus_tag="ID725_RS06270"
                     /old_locus_tag="pgaptmp_001254"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGV7849892.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            complement(499..1896)
                     /gene="pcnB"
                     /locus_tag="ID725_RS06275"
                     /old_locus_tag="pgaptmp_001255"
     CDS             complement(499..1896)
                     /gene="pcnB"
                     /locus_tag="ID725_RS06275"
                     /old_locus_tag="pgaptmp_001255"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGV7849893.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1956..2852)
                     /gene="gluQRS"
                     /locus_tag="ID725_RS06280"
                     /old_locus_tag="pgaptmp_001256"
     CDS             complement(1956..2852)
                     /gene="gluQRS"
                     /locus_tag="ID725_RS06280"
                     /old_locus_tag="pgaptmp_001256"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGV7849894.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2919..3374)
                     /gene="dksA"
                     /locus_tag="ID725_RS06285"
                     /old_locus_tag="pgaptmp_001257"
     CDS             complement(2919..3374)
                     /gene="dksA"
                     /locus_tag="ID725_RS06285"
                     /old_locus_tag="pgaptmp_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGV7849895.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3552..4256)
                     /gene="sfsA"
                     /locus_tag="ID725_RS06290"
                     /old_locus_tag="pgaptmp_001258"
     CDS             complement(3552..4256)
                     /gene="sfsA"
                     /locus_tag="ID725_RS06290"
                     /old_locus_tag="pgaptmp_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGV7849896.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4271..4801)
                     /gene="thpR"
                     /locus_tag="ID725_RS06295"
                     /old_locus_tag="pgaptmp_001259"
     CDS             complement(4271..4801)
                     /gene="thpR"
                     /locus_tag="ID725_RS06295"
                     /old_locus_tag="pgaptmp_001259"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGV7849897.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            4875..7304
                     /gene="hrpB"
                     /locus_tag="ID725_RS06300"
                     /old_locus_tag="pgaptmp_001260"
     CDS             4875..7304
                     /gene="hrpB"
                     /locus_tag="ID725_RS06300"
                     /old_locus_tag="pgaptmp_001260"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGV7849898.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7500..10034
                     /gene="mrcB"
                     /locus_tag="ID725_RS06305"
                     /old_locus_tag="pgaptmp_001261"
     CDS             7500..10034
                     /gene="mrcB"
                     /locus_tag="ID725_RS06305"
                     /old_locus_tag="pgaptmp_001261"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGV7849899.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10254..12497
                     /gene="fhuA"
                     /locus_tag="ID725_RS06310"
                     /old_locus_tag="pgaptmp_001262"
     CDS             10254..12497
                     /gene="fhuA"
                     /locus_tag="ID725_RS06310"
                     /old_locus_tag="pgaptmp_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGV7849900.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12548..13345
                     /gene="fhuC"
                     /locus_tag="ID725_RS06315"
                     /old_locus_tag="pgaptmp_001263"
     CDS             12548..13345
                     /gene="fhuC"
                     /locus_tag="ID725_RS06315"
                     /old_locus_tag="pgaptmp_001263"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGV7849901.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13345..14235
                     /gene="fhuD"
                     /locus_tag="ID725_RS06320"
                     /old_locus_tag="pgaptmp_001264"
     CDS             13345..14235
                     /gene="fhuD"
                     /locus_tag="ID725_RS06320"
                     /old_locus_tag="pgaptmp_001264"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGV7849902.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14232..16214
                     /gene="fhuB"
                     /locus_tag="ID725_RS06325"
                     /old_locus_tag="pgaptmp_001265"
     CDS             14232..16214
                     /gene="fhuB"
                     /locus_tag="ID725_RS06325"
                     /old_locus_tag="pgaptmp_001265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGV7849903.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16249..17529)
                     /gene="hemL"
                     /locus_tag="ID725_RS06330"
                     /old_locus_tag="pgaptmp_001266"
     CDS             complement(16249..17529)
                     /gene="hemL"
                     /locus_tag="ID725_RS06330"
                     /old_locus_tag="pgaptmp_001266"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGV7849904.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17695..17742
                     /gene="yadX"
                     /locus_tag="ID725_RS06335"
                     /old_locus_tag="pgaptmp_001267"
     CDS             17695..17742
                     /gene="yadX"
                     /locus_tag="ID725_RS06335"
                     /old_locus_tag="pgaptmp_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGV7849905.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17754..19175
                     /gene="clcA"
                     /locus_tag="ID725_RS06340"
                     /old_locus_tag="pgaptmp_001268"
     CDS             17754..19175
                     /gene="clcA"
                     /locus_tag="ID725_RS06340"
                     /old_locus_tag="pgaptmp_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGV7849906.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19199..19264
                     /gene="yadW"
                     /locus_tag="ID725_RS06345"
                     /old_locus_tag="pgaptmp_001269"
     CDS             19199..19264
                     /gene="yadW"
                     /locus_tag="ID725_RS06345"
                     /old_locus_tag="pgaptmp_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGV7849907.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19257..19601
                     /gene="erpA"
                     /locus_tag="ID725_RS06350"
                     /old_locus_tag="pgaptmp_001270"
     CDS             19257..19601
                     /gene="erpA"
                     /locus_tag="ID725_RS06350"
                     /old_locus_tag="pgaptmp_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGV7849908.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19648..20271)
                     /gene="yadS"
                     /locus_tag="ID725_RS06355"
                     /old_locus_tag="pgaptmp_001271"
     CDS             complement(19648..20271)
                     /gene="yadS"
                     /locus_tag="ID725_RS06355"
                     /old_locus_tag="pgaptmp_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGV7849909.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20309..21109)
                     /gene="btuF"
                     /locus_tag="ID725_RS06360"
                     /old_locus_tag="pgaptmp_001272"
     CDS             complement(20309..21109)
                     /gene="btuF"
                     /locus_tag="ID725_RS06360"
                     /old_locus_tag="pgaptmp_001272"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGV7849910.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21102..21800)
                     /gene="mtnN"
                     /locus_tag="ID725_RS06365"
                     /old_locus_tag="pgaptmp_001273"
     CDS             complement(21102..21800)
                     /gene="mtnN"
                     /locus_tag="ID725_RS06365"
                     /old_locus_tag="pgaptmp_001273"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGV7849911.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21884..23401
                     /gene="dgt"
                     /locus_tag="ID725_RS06370"
                     /old_locus_tag="pgaptmp_001274"
     CDS             21884..23401
                     /gene="dgt"
                     /locus_tag="ID725_RS06370"
                     /old_locus_tag="pgaptmp_001274"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGV7849912.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23531..24955
                     /gene="degP"
                     /locus_tag="ID725_RS06375"
                     /old_locus_tag="pgaptmp_001275"
     CDS             23531..24955
                     /gene="degP"
                     /locus_tag="ID725_RS06375"
                     /old_locus_tag="pgaptmp_001275"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGV7849913.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKLISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25110..26267
                     /gene="cdaR"
                     /locus_tag="ID725_RS06380"
                     /old_locus_tag="pgaptmp_001276"
     CDS             25110..26267
                     /gene="cdaR"
                     /locus_tag="ID725_RS06380"
                     /old_locus_tag="pgaptmp_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HGV7849914.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26356..26742)
                     /gene="yaeH"
                     /locus_tag="ID725_RS06385"
                     /old_locus_tag="pgaptmp_001277"
     CDS             complement(26356..26742)
                     /gene="yaeH"
                     /locus_tag="ID725_RS06385"
                     /old_locus_tag="pgaptmp_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGV7849915.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27057..27881)
                     /gene="dapD"
                     /locus_tag="ID725_RS06390"
                     /old_locus_tag="pgaptmp_001278"
     CDS             complement(27057..27881)
                     /gene="dapD"
                     /locus_tag="ID725_RS06390"
                     /old_locus_tag="pgaptmp_001278"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGV7849916.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27912..30584)
                     /gene="glnD"
                     /locus_tag="ID725_RS06395"
                     /old_locus_tag="pgaptmp_001279"
     CDS             complement(27912..30584)
                     /gene="glnD"
                     /locus_tag="ID725_RS06395"
                     /old_locus_tag="pgaptmp_001279"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGV7849917.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30646..31440)
                     /gene="map"
                     /locus_tag="ID725_RS06400"
                     /old_locus_tag="pgaptmp_001280"
     CDS             complement(30646..31440)
                     /gene="map"
                     /locus_tag="ID725_RS06400"
                     /old_locus_tag="pgaptmp_001280"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGV7849918.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31808..32533
                     /gene="rpsB"
                     /locus_tag="ID725_RS06405"
                     /old_locus_tag="pgaptmp_001281"
     CDS             31808..32533
                     /gene="rpsB"
                     /locus_tag="ID725_RS06405"
                     /old_locus_tag="pgaptmp_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGV7849919.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32791..33642
                     /gene="tsf"
                     /locus_tag="ID725_RS06410"
                     /old_locus_tag="pgaptmp_001282"
     CDS             32791..33642
                     /gene="tsf"
                     /locus_tag="ID725_RS06410"
                     /old_locus_tag="pgaptmp_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGV7849920.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33789..34514
                     /gene="pyrH"
                     /locus_tag="ID725_RS06415"
                     /old_locus_tag="pgaptmp_001283"
     CDS             33789..34514
                     /gene="pyrH"
                     /locus_tag="ID725_RS06415"
                     /old_locus_tag="pgaptmp_001283"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGV7849921.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34664..35221
                     /gene="frr"
                     /locus_tag="ID725_RS06420"
                     /old_locus_tag="pgaptmp_001284"
     CDS             34664..35221
                     /gene="frr"
                     /locus_tag="ID725_RS06420"
                     /old_locus_tag="pgaptmp_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGV7849922.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35313..36509
                     /gene="ispC"
                     /locus_tag="ID725_RS06425"
                     /old_locus_tag="pgaptmp_001285"
     CDS             35313..36509
                     /gene="ispC"
                     /locus_tag="ID725_RS06425"
                     /old_locus_tag="pgaptmp_001285"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGV7849923.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            36698..37456
                     /gene="ispU"
                     /locus_tag="ID725_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /gene_synonym="uppS"
     CDS             36698..37456
                     /gene="ispU"
                     /locus_tag="ID725_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGV7849924.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37469..38326
                     /gene="cdsA"
                     /locus_tag="ID725_RS06435"
                     /old_locus_tag="pgaptmp_001287"
     CDS             37469..38326
                     /gene="cdsA"
                     /locus_tag="ID725_RS06435"
                     /old_locus_tag="pgaptmp_001287"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGV7849925.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38338..39690
                     /gene="rseP"
                     /locus_tag="ID725_RS06440"
                     /old_locus_tag="pgaptmp_001288"
     CDS             38338..39690
                     /gene="rseP"
                     /locus_tag="ID725_RS06440"
                     /old_locus_tag="pgaptmp_001288"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGV7849926.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39720..42152
                     /gene="bamA"
                     /locus_tag="ID725_RS06445"
                     /old_locus_tag="pgaptmp_001289"
     CDS             39720..42152
                     /gene="bamA"
                     /locus_tag="ID725_RS06445"
                     /old_locus_tag="pgaptmp_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGV7849927.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42273..42758
                     /gene="skp"
                     /locus_tag="ID725_RS06450"
                     /old_locus_tag="pgaptmp_001290"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42273..42758
                     /gene="skp"
                     /locus_tag="ID725_RS06450"
                     /old_locus_tag="pgaptmp_001290"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGV7849928.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42762..43787
                     /gene="lpxD"
                     /locus_tag="ID725_RS06455"
                     /old_locus_tag="pgaptmp_001291"
     CDS             42762..43787
                     /gene="lpxD"
                     /locus_tag="ID725_RS06455"
                     /old_locus_tag="pgaptmp_001291"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGV7849929.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43892..44347
                     /gene="fabZ"
                     /locus_tag="ID725_RS06460"
                     /old_locus_tag="pgaptmp_001292"
     CDS             43892..44347
                     /gene="fabZ"
                     /locus_tag="ID725_RS06460"
                     /old_locus_tag="pgaptmp_001292"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGV7849930.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44351..45139
                     /gene="lpxA"
                     /locus_tag="ID725_RS06465"
                     /old_locus_tag="pgaptmp_001293"
     CDS             44351..45139
                     /gene="lpxA"
                     /locus_tag="ID725_RS06465"
                     /old_locus_tag="pgaptmp_001293"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGV7849931.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45139..46287
                     /gene="lpxB"
                     /locus_tag="ID725_RS06470"
                     /old_locus_tag="pgaptmp_001294"
     CDS             45139..46287
                     /gene="lpxB"
                     /locus_tag="ID725_RS06470"
                     /old_locus_tag="pgaptmp_001294"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGV7849932.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46284..46880
                     /gene="rnhB"
                     /locus_tag="ID725_RS06475"
                     /old_locus_tag="pgaptmp_001295"
     CDS             46284..46880
                     /gene="rnhB"
                     /locus_tag="ID725_RS06475"
                     /old_locus_tag="pgaptmp_001295"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGV7849933.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46917..50399
                     /gene="dnaE"
                     /locus_tag="ID725_RS06480"
                     /old_locus_tag="pgaptmp_001296"
     CDS             46917..50399
                     /gene="dnaE"
                     /locus_tag="ID725_RS06480"
                     /old_locus_tag="pgaptmp_001296"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGV7849934.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50412..51371
                     /gene="accA"
                     /locus_tag="ID725_RS06485"
                     /old_locus_tag="pgaptmp_001297"
     CDS             50412..51371
                     /gene="accA"
                     /locus_tag="ID725_RS06485"
                     /old_locus_tag="pgaptmp_001297"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGV7849935.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51470..53611
                     /gene="ldcC"
                     /locus_tag="ID725_RS06490"
                     /old_locus_tag="pgaptmp_001298"
     CDS             51470..53611
                     /gene="ldcC"
                     /locus_tag="ID725_RS06490"
                     /old_locus_tag="pgaptmp_001298"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGV7849936.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53668..54057
                     /gene="yaeR"
                     /locus_tag="ID725_RS06495"
                     /old_locus_tag="pgaptmp_001299"
     CDS             53668..54057
                     /gene="yaeR"
                     /locus_tag="ID725_RS06495"
                     /old_locus_tag="pgaptmp_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGV7849937.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54122..55417
                     /gene="tilS"
                     /locus_tag="ID725_RS06500"
                     /old_locus_tag="pgaptmp_001300"
     CDS             54122..55417
                     /gene="tilS"
                     /locus_tag="ID725_RS06500"
                     /old_locus_tag="pgaptmp_001300"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGV7849938.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(55470..55730)
                     /gene="rof"
                     /locus_tag="ID725_RS06505"
                     /old_locus_tag="pgaptmp_001301"
     CDS             complement(55470..55730)
                     /gene="rof"
                     /locus_tag="ID725_RS06505"
                     /old_locus_tag="pgaptmp_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGV7849939.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55717..55917)
                     /gene="yaeP"
                     /locus_tag="ID725_RS06510"
                     /old_locus_tag="pgaptmp_001302"
     CDS             complement(55717..55917)
                     /gene="yaeP"
                     /locus_tag="ID725_RS06510"
                     /old_locus_tag="pgaptmp_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGV7849940.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56083..56628
                     /gene="yaeQ"
                     /locus_tag="ID725_RS06515"
                     /old_locus_tag="pgaptmp_001303"
     CDS             56083..56628
                     /gene="yaeQ"
                     /locus_tag="ID725_RS06515"
                     /old_locus_tag="pgaptmp_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGV7849941.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56625..57035
                     /gene="arfB"
                     /locus_tag="ID725_RS06520"
                     /old_locus_tag="pgaptmp_001304"
     CDS             56625..57035
                     /gene="arfB"
                     /locus_tag="ID725_RS06520"
                     /old_locus_tag="pgaptmp_001304"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGV7849942.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            57049..57759
                     /gene="nlpE"
                     /locus_tag="ID725_RS06525"
                     /old_locus_tag="pgaptmp_001305"
                     /gene_synonym="cutF"
     CDS             57049..57759
                     /gene="nlpE"
                     /locus_tag="ID725_RS06525"
                     /old_locus_tag="pgaptmp_001305"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGV7849943.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57959..58783)
                     /gene="yaeF"
                     /locus_tag="ID725_RS06530"
                     /old_locus_tag="pgaptmp_001306"
     CDS             complement(57959..58783)
                     /gene="yaeF"
                     /locus_tag="ID725_RS06530"
                     /old_locus_tag="pgaptmp_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HGV7849944.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58836..60554)
                     /gene="proS"
                     /locus_tag="ID725_RS06535"
                     /old_locus_tag="pgaptmp_001307"
     CDS             complement(58836..60554)
                     /gene="proS"
                     /locus_tag="ID725_RS06535"
                     /old_locus_tag="pgaptmp_001307"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGV7849945.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60665..61372)
                     /gene="tsaA"
                     /locus_tag="ID725_RS06540"
                     /old_locus_tag="pgaptmp_001308"
     CDS             complement(60665..61372)
                     /gene="tsaA"
                     /locus_tag="ID725_RS06540"
                     /old_locus_tag="pgaptmp_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGV7849946.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61369..61773)
                     /gene="rcsF"
                     /locus_tag="ID725_RS06545"
                     /old_locus_tag="pgaptmp_001309"
     CDS             complement(61369..61773)
                     /gene="rcsF"
                     /locus_tag="ID725_RS06545"
                     /old_locus_tag="pgaptmp_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGV7849947.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            complement(61891..62706)
                     /gene="metQ"
                     /locus_tag="ID725_RS06550"
                     /old_locus_tag="pgaptmp_001310"
     CDS             complement(61891..62706)
                     /gene="metQ"
                     /locus_tag="ID725_RS06550"
                     /old_locus_tag="pgaptmp_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGV7849948.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62746..63399)
                     /gene="metI"
                     /locus_tag="ID725_RS06555"
                     /old_locus_tag="pgaptmp_001311"
     CDS             complement(62746..63399)
                     /gene="metI"
                     /locus_tag="ID725_RS06555"
                     /old_locus_tag="pgaptmp_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGV7849949.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63392..64423)
                     /gene="metN"
                     /locus_tag="ID725_RS06560"
                     /old_locus_tag="pgaptmp_001312"
     CDS             complement(63392..64423)
                     /gene="metN"
                     /locus_tag="ID725_RS06560"
                     /old_locus_tag="pgaptmp_001312"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGV7849950.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64611..65186
                     /gene="gmhB"
                     /locus_tag="ID725_RS06565"
                     /old_locus_tag="pgaptmp_001313"
     CDS             64611..65186
                     /gene="gmhB"
                     /locus_tag="ID725_RS06565"
                     /old_locus_tag="pgaptmp_001313"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGV7849951.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15396 a        16250 c        18039 g        15638 t
ORIGIN      
        1 agaaaataaa ataatacagc taataccaga tggcaggttg tagtactgct agcgaagtga
       61 gtatttcacg taatgtctcc ccatcaggaa acacgagttc cggggcaatc tcaaacagcg
      121 gccacagcat aaagccgcga ttcttcatat cgtaatgcgg gacggtgagt cgttcggtat
      181 taatggtttc attaccaaac aacatgatat caagatcgag tgtgcgcggt ccccagcgtt
      241 ctgctttacg aacgcgacct tgctgcaact ctatttgctg tgtgtgatta agtaattctt
      301 caggcgcaag tgttgtttcc agcgccacgg ctgcgtttaa gtaatcgggt tgatcttgcg
      361 gccccagcgg tggggtgcgg taaaacgaag aaacggtaag aatgtggctt tcagggatat
      421 cgcctaatgc tttcagggca gcattgacct gctccagcgg agaggccaga ttgctgccta
      481 tggcaatata cgccactgtc atgcggtacc ctcacgacgt ggtgcgcgtt tgcgtggacg
      541 acgagtacga cgacgcggtg acggttcttc atccagctcg ttgagcatcc ctttttggtc
      601 tggtggcgcg gaaacctgga actcacccca ccatttcacc agacgctgca gttcagcgtt
      661 acgctcaact tcagctcgca aggccaacag gtcataagcc gcacggaact taggatgctc
      721 cagcaatttc catgcgcgtt taccctgacg tcgggacata cgcaactgca actgccagat
      781 atcgcgggtt aacgtcgtca gacgtttcgg gattgccagt gaacggcagg cttcgtccag
      841 cacgtcgttc atcgccagcg cgaaagcgtc gtgataggtc aggccgcttt cctgggcgat
      901 cttctgtgcc gtctccagca gtgggtacca gaacatggcg gcaaacagga acgccgggtt
      961 cacgcgcata tcgttatgga tacgcgtatc ggtattcttc agcacctgtt caatgatccg
     1021 ctccatcggg ctgtcgccat tttccgtgaa gtagcgggta atggtcggga acagcggctg
     1081 gaacagatga tattcacaca acagcttata ggtttcgtaa ccgtagcccg cttgtagcag
     1141 tttaagcgat tcttcaaaca ggcgtgccgg tgggatatcg ttcagcaggg tagcgaggcg
     1201 agggatcggt tctgcggttt ccgggctgat gcgcataccc aatttggcgg caaaacgtac
     1261 tgcgcgcagc atacgtaccg gatcttcacg gtagcgcgtt tccgggttac caatcagacg
     1321 gataacaccg tccttcagat ccttcatacc gccaacgtaa tcacggacgg taaaatccgc
     1381 tacgctgtaa tacaggctgt tgatagtgaa atcgcggcgc tgggcgtctt cttcgatgga
     1441 gccgaaaatg ttgtcgcgca gcaacatgcc gttttgcccg cgttgggagg tcgtgcggtc
     1501 gctgacgtta ccttcgtggt gtccacggaa ggtcgcaact tcgataatct ccgggccaaa
     1561 catcacatga gccagacgga aacggcgacc caccaggcgg cagttacgga acagtttgcg
     1621 cacctgctca ggcgtggcgt tagtggttac gtcaaaatct ttcggctttt tgccgagtaa
     1681 caggtcgcgc acgccgccgc caaccagcca ggcttcgtat cccgctttat tgagcctgta
     1741 cattaccttc agggcatttt cactgatatc tttgcgggaa atagcatgct gctcacgcgg
     1801 gatcaccgtc acctgtggac gggcgactgc ctgttcagcc tcgctttcct cgcggcttag
     1861 caccttgcgg caaaaattag cgactcgggt aaaaatagta cacctcggta gtgtcaaaca
     1921 tcattcagga caaaaaaata gcggctaatc atagctcagc atgacgcatt tgagaatgtt
     1981 gaatttacaa ttgccgactc aggcactgcg gtaagccgcc agtttttgac ggctgactga
     2041 aggatttgct cgacgctgaa atcctgccag tgtgcttctg cctgctgccc cagaaattga
     2101 agtgccgcga ttagtaccgg gcgtggatcg cctttcggca acgcaggcgc atgattctgc
     2161 ttggaaagtt tagcgccttg tggattaagc gccagcggca gatgaatgta atctggcact
     2221 ttccagccaa aaagctggta cagcgagatt tgccgcactg tcggttcgat caaatctgcc
     2281 ccgcggacaa tttcgctcac gccctggaag tgatcatcca ccaccaccgc caggttatag
     2341 gcaaacaaac catcacggcg atgaataata aaatcttccc gtgccagttt ttcgtcggcg
     2401 tgaataatgc cgcgcagctg gtcagtaaat tgcgtgaccg gatgctgctg gcggatacgc
     2461 actgcggcgt tgtctggtcc atgatgcaac acccggcaat gaccgtcgta aataccgcca
     2521 atgctttgaa tacgcgcacg cgtgcaggtg cagtaataac ttagtccttg ttcatgtaac
     2581 caggcgagtg cttcacgata ggcgtggtga cgttgcgatt gccagagaac atcgccgtcc
     2641 cagtgcagac cgtaatgttc cagctggcgc aggatagttt ctgcggcacc gggaacttca
     2701 cgaggcgggt cgatatcttc aatgcgtacc agccagcgac cgtgccgggc gcgagcctgc
     2761 aaatagctgc caagcgcagc aatcagagaa ccaaaatgaa gctcgccgga aggagagggg
     2821 gcgaagcggc caatatactg tgtgtctgtc atctctttga acaaaaaata aggcgggagc
     2881 atttcccgcc tgtggtaaac gtgatggaac ggctgtaatt agccagccat ctgtttttcg
     2941 cgaatttcag ccagcgtttt gcagtcgatg cacagatcgg ctgtcgggcg cgcttccaga
     3001 cggcgaatac caatttcaac accgcaggat tcacagtagc cgaaatcttc gtcttccact
     3061 tttttcagcg tcttctcgat ctttttgatc agcttacgct cgcgatcgcg gttacgcagt
     3121 tcgaggctga actcttcttc ctgggctgca cggtctaccg ggtccgggaa attggctgct
     3181 tcatcctgca tatgtgtaac ggtgcgatcg acttcatccc tgagttgatt acgccatgct
     3241 tccagaatac gacggaagtg cgccagctgg gcttcattca tatactcttc gcccggcttc
     3301 tcctgatatg gttccacccc agcgatggcg agaatactca gggacgatgt tttacggttt
     3361 tgcccttctt gcatgttgct tctccttaac acgcactatc gatccccatg ttcgggggaa
     3421 aaatgaggcc gctataaata gcagatgctt ttccggatag caattatcta aacgtaacac
     3481 ttgacaactg tgtgaggaaa agcgtatttg cgcacgcgac cagaatgtaa attaaccagt
     3541 tacttacttt actacaatgt aaccggcagt gattttttaa gagccatgcc ttcagcagaa
     3601 atttccgctt tgtaagccag aatttctacc cccctctgtt gagcttctga caatagttgc
     3661 gcgtatttct catcgatgtg gcgcgcgggt gaaaaccgtg taatggctga atgcagcacg
     3721 gcgaaaaaga taaccgcacg ctggccttca gccgctacgc tcatcaactc ccgaaggtgt
     3781 ttctgacctc gttcagtgac cgcatcggga aaatatccct gttcgttctc cgctaacgta
     3841 accgatttca cttcaatata gcagtctgga cgcgaatccg cctgcaacat aaagtcaata
     3901 cggctgcgtt ctgcgccgta ttttacttcg cttttcagcg agctatagcc tgacagttct
     3961 gaaattgatt cattaaggat agcctctttc gtcaacctgt tagcccaaag cgtgttgacg
     4021 caaataaatg cgccgctctg gctttgagtt aattcccagg tgtgtgggta tttccgtttg
     4081 gtgttgtctg aagtcgaata ccagacggta tcgccaggcg ttgcacaacc ggtcatcgca
     4141 cccgtattcg ggcagtgtag cgttaattcg cgaccatcgg gtgtgatcac atcggctaaa
     4201 aaacgtttgt aacgctgaat tagcgtcgcg cgctgtagag ggggagaaaa ttccattgcg
     4261 acaatccttg ttattgcgtt agcacccagc gttttagcgg cgtgtagcgt gtgcgtccac
     4321 gggcaaacga ggaggcgtaa agggtgaact ccgtcaccgc atatgaccag ttaaaacctg
     4381 gcggcgggat tgtcaccgcc tcgctggcgt cgcgcaataa ggtaatatgt ggatgaaacg
     4441 gacgattgct ttgaaaacaa ccgctgcggg cagcctgtga acggagcata ttcgccagct
     4501 ggattaagcc gcgtggcggc tgacgcatcc ctaaccacac cacacgcgaa cgcagccatt
     4561 ggccggcgtc atcaagcgtg agtgtgaaac caggttgacg aatccgtccg gctaaaagag
     4621 aaagcgcctt ctctttctct gcgctcactt cgcctaaaaa tgccagagtc agatgcaaat
     4681 tatcggcggc gaccggacgt cccgcctcaa gtgggaagtg tgtggcgcgc cagtggataa
     4741 tctgttcgcg gatttctgca ggtaagtcga tagcaaagaa cagacgttgc gggtcagaca
     4801 tgtgaggcac tcggttatga attaccgcga tgctacaatg tggcgcgaag aatgttaacc
     4861 ctctggagcg ttttgtgtcg tcgttgcccg ttgctgccgt gttacctgaa ttacttaccg
     4921 ctctcgatta tgcgccgcag gtattattaa gtgcgccgac cggggccggg aaatcaacct
     4981 ggctgccgct gcaactgctg gcgcatcccg gcattaacgg gaaaattatc ctcctggagc
     5041 cacggcgtct ggcggcgcgt aacgtcgcgc aacggctggc ggagctgctt aacgaaaagc
     5101 caggcgatac cgttggctac cggatgcgtg cgcaaaactg cgtcgggccg aatacccgcc
     5161 tggaagtagt taccgaaggc gtgctgacgc ggatgatcca gcgtgacccg gaactgagcg
     5221 gtgttggatt ggtgatcctt gatgaatttc atgagcgtag cttgcaggcg gatttggcgt
     5281 tggcgctgtt actcgatgtg caacaaggtc tgcgtgatga ccttaaactg ctgattatgt
     5341 cggctacgct ggacaacgac cgcttgcagc aaatgctgcc agaagcgccc gtcgtcatct
     5401 cagaagggcg ctcgtttccg gttgaacgcc gttatttacc gctgcccacg catcagcgtt
     5461 ttgacgatgc cgttgcggta gccaccgctg aaatgctgcg tcaggaaagc ggatcattac
     5521 tgttattttt acctggcgtc ggagaaattc agcgtgtgca ggaacaactg gcttcgcgca
     5581 tcggcagtga tgtattgctc tgcccgctgt atggcgcgtt gtcgctgaac gatcagcgaa
     5641 aagcgatcct cccggcaccg caagggatgc gcaaagtggt gctggcgacc aatattgctg
     5701 aaaccagttt aaccattgaa ggtattcgtc tggtggtgga ttgtgcccag gaaagggtgg
     5761 cgcgttttga tccgcgaaca gggcttacgc ggttgattac tcagcgaatc agtcaggcgt
     5821 cgatgacgca gcgtgccggg cgcgccgggc gtctggagcc gggtatctgc ctgcatttaa
     5881 tcgccaaaga acaagcagaa cgcgctaccg cgcaaagtga accggagatc ttacaaagcg
     5941 atctttccgg tttgctgatg gaattactgc aatggggatg cagcgatccg gcgcagatga
     6001 gctggctgga tcaaccgcca acggtgaatc tactggccgc gaaacgtctg ttacaaatgc
     6061 tgggggcgct ggacggtgaa cggcttagtg cgcaagggca aaaaatggca gcgctgggta
     6121 acgatccgcg tttagcggca atgctggtta gcgcaaagag cgacgacgaa gctgctaccg
     6181 cggcaaaaat tgccgccatt ctcgaagagc cgccacggat gggcaatagt gacctgggcg
     6241 tggcgttttc gcgcaatcaa ccagcctggc agcaacgtag tcagcaactg ttaaaacgct
     6301 taaacgtacg cggcggtgag gcagacagtt cgcttatcgc gccgctactt gccggggcgt
     6361 ttgccgatcg cattgctcgc cgccgtgggc aagatggacg ctatcaactg gcaaacggca
     6421 tgggggcgat gcttgatgcc gacgacgcgc taagtcgcca cgaatggttg atcgcgccgt
     6481 tattattgca gggcagcgcc tcgccggatg cgcggatttt actggcgtta ccggtcgata
     6541 ttgatgaatt agtacaacgc tgtccgcagc tggtgcagca atctgacacc gtggaatggg
     6601 atgacgcgca aggtacgctg aaagcctggc gtcggctgca aatcggtcag ttgacggtga
     6661 aagtgcagcc gttggcgaaa ccgtcagaag acgagttgca tcaggcgatg cttaacggca
     6721 tccgtgataa aggtttaagc gtgctcaact ggacggcgga agcggaacag ctacgcttgc
     6781 gtttgttatg cgccggaaag tggttgccag aatatgactg gccagcggtt gatgatgaaa
     6841 gtttgttggc gacgctggaa acgtggctgc tgccacatat ggccggtgta cattcactac
     6901 gcggcctgaa atcactcgac atttatcagg cactacgcgg attacttgat tggggaatgc
     6961 agcaacgtct ggatagtgaa ttgcctgcgc attacactgt gccgacggga agccggatcg
     7021 ccattcgtta tcatgaagat aacccgcccg cgctggcggt gagaatgcag gagatgtttg
     7081 gtgaggccac taatccgacg atcgcccagg ggcgcgtgcc gctggtgctg gaattgcttt
     7141 cacccgccca aaggccgctg caaatcacgc gtgatttggg ggccttctgg aaaggggcgt
     7201 atcgtgaggt gcaaaaggag atgaaagggc gttatcccaa acacgtctgg ccggacgacc
     7261 cggcaaacac ggcaccgacg cgacggacga aaaagtattc gtaaggttgg ttttctccct
     7321 ctccctgtgg gagagggccg gggtgagggc accagcgcgc acgttcaccc tcaccctaac
     7381 cctctccctt aagggagagg ggaccgatcg agctcaaatt ttgagagata tcttcttctg
     7441 tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca
     7501 tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt ccggtcaaac
     7561 aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat gatgactatg
     7621 aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg
     7681 gcaaacgcgg ctggctatgg ctgctgttaa aactggctat cgtttttgcc gtgctgatcg
     7741 ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc aaggtctggc
     7801 aactgcctgc ggcagtttat ggtcgaatgg tcaaccttga gccagacatg accatcagca
     7861 agaacgagat ggtgaagctg ctggaggcaa cacagtatcg tcaggtgtcg aaaatgaccc
     7921 gtcctggcga atttaccgtg caggccaaca gcattgagat gattcgccgt ccgtttgatt
     7981 tcccggacag taaagaaggg caggtgcgcg cgcgtctgac ctttgatggc gatcatctgg
     8041 cgaccatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt gatccgcgtc
     8101 tgatcaccat gatctcctca ccaaacggtg agcagcgttt gtttgtgccg cgcagtggtt
     8161 tcccggattt gctggtggat actttgctgg cgacagaaga ccgccatttc tacgagcatg
     8221 atggaatcag tctctactcc atcggacgtg cggtgctggc aaacctgacc gcaggtcgca
     8281 cggtgcaggg ggcgagtacg ctgacgcaac agctggtgaa aaacctgttc ctctccagcg
     8341 agcgctctta ttggcgtaaa gcgaatgaag cctacatggc gctgatcatg gacgcgcgtt
     8401 acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt cagagcggcg
     8461 acaacgaaat ccgcggcttc ccgctggcga gcttgtatta ctttggtcgc ccggtagaag
     8521 aattgagcct cgatcagcag gcgctgttag tcggtatggt gaaaggagcg tccatctaca
     8581 acccgtggcg taacccgaaa ctggcgctgg agcgacgtaa tctggtactg cgtctgctgc
     8641 aacagcaaca gattattgat caagaactct atgacatgtt gagtgcgcgt ccgctggggg
     8701 ttcagccgcg cggtggggtg atctctcctc agccagcctt tatgcaactg gtgcgtcagg
     8761 agcttcaggc taaattaggc gataaggtaa aagatctctc cggcgtgaag atcttcacta
     8821 cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggt attccggcac
     8881 tgaagaaaca gcgtaagttg agcgatcttg aaactgcgat tgtggtcgtc gaccgcttta
     8941 gtggtgaagt tcgtgcgatg gtcggaggtt ctgagccgca gtttgcgggc tacaaccgtg
     9001 cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat ctgacggcct
     9061 taagccagcc gaaaatctat cgtctgaata cgtggattgc ggatgcgcca attgcgctgc
     9121 gtcagccgaa tggccaggtc tggtcaccgc agaatgatga ccgtcgttat agcgaaagcg
     9181 gcagagtgat gctggtggat gcgttgaccc gttcgatgaa cgtgccgacg gtaaatctgg
     9241 ggatggcgct ggggctgcct gcggttacgg atacctggat taaactgggc gtaccgaaag
     9301 atcagttgca tccggttccg gcaatgctgc tgggggcgtt gaacttaacg ccaatcgaag
     9361 tggcgcaggc attccagacc atcgccagcg gtggtaaccg tgcaccgctt tctgcgctgc
     9421 gttcggtaat cgcggaagat ggcaaagtgc tgtatcagag cttcccgcag gcggaacgcg
     9481 ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg gtacaacgcg
     9541 gtacgggtcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg aaaacaggga
     9601 ctaccaacaa taacgtagat acctggtttg cgggcattga cggcagcacg gtgaccatca
     9661 cctgggtcgg ccgtgataac aaccagccga ccaaactgta tggagccagc ggggcaatgt
     9721 cgatttatca gcgttatctg gctaaccaga cgccaacgcc gctgaatctt gttccgccag
     9781 aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc ggtggcatgc
     9841 gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagt gagatgcagc
     9901 agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag ccgcaacagc
     9961 aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatt aaggatatgt
    10021 ttggtagtaa ttaacatcta agcgtgaaat accggatggc gagttgccat ccggtaaaat
    10081 aacatcccac ccaaagtatt aaccctttct tttcatctgg ttgtttatta acccttcagg
    10141 aacgctcaga ttgcgtaccg cttgcgaacc cgccagagtt tcgaatatta tcttatcttt
    10201 ataataatca ttctcgttta cgttatcatt cactttacat cagagatata ccaatggcgc
    10261 gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta gtagccacag
    10321 cggttagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa gacactatca
    10381 ccgttaccgc tgcacctgcg ccgcaagaaa gcgcatgggg gcctgctgca actattgcgg
    10441 cgcgacagtc agctaccggc actaaaaccg atacgccgat tcaaaaagtg ccacagtcta
    10501 tttctgttgt gaccgccgaa gagatggcgc tgcatcagcc gaagtcggta aaagaagcgc
    10561 ttagctacac gccgggtgtc tctgttggta cgcgtggcgc atccaacacc tatgaccacc
    10621 tgatcattcg cggttttgcg gcagaaggcc aaagccagaa taactatctg aatggcctga
    10681 agttgcaggg caacttctat aacgatgcgg tcattgatcc gtatatgctg gaacgcgctg
    10741 aaattatgcg tggcccggtt tccgtgcttt acggtaaaag cagtcctggc ggcctgttga
    10801 atatggtcag caagcgtccg accaccgaac cgctgaaaga agttcagttt aaagccggta
    10861 ctgacagcct gttccagact ggttttgact ttagcgatgc gctggatgat gacggcgttt
    10921 actcttatcg cctgaccggt cttgcgcgtt ctgccaatgc ccagcagaaa gggtcagaag
    10981 agcagcgtta tgctattgca ccggcgttca cctggcgtcc ggatgataaa accaatttca
    11041 ccttcctttc ttacttccag aacgagccgg aaaccggtta ttacggctgg ttgccgaaag
    11101 agggaaccgt tgagccgctg ccgaacggta agcgtctgcc gacagacttt aacgaagggg
    11161 cgaagaacaa cacctattct cgtaatgaga agatggtggg ctacagcttc gatcacgaat
    11221 ttaacgacac ctttactgtg cgtcagaacc tgcgctttgc tgaaaacaaa acctcgcaaa
    11281 acagcgttta tggttacggc gtctgctccg atccggcgaa tgcttacagc aaacagtgtg
    11341 cggcattagc gccagcggat aaaggccatt atctggcacg taaatacgtc gttgatgatg
    11401 agaagctgca aaacttctcc gttgataccc agttgcagag caagtttgcc actggcgata
    11461 tcgaccacac cctgctgacc ggtgtcgact ttatgcgtat gcgtaatgac atcaacgcct
    11521 ggtttggtta cgacgactcc gtaccgctgc tcgatctgta caatccggtg aataccgatt
    11581 tcgacttcaa tgccaaagat ccggcaaact ccggccctta ccgcattctg aataagcaga
    11641 aacaaacggg cgtttatgtt caggatcagg cgcagtggga taaagtgctg gtcaccctgg
    11701 gcggtcgtta tgactgggca gatcaagaat ctcttaaccg cgttgccggg acgaccgata
    11761 aacgtgatga caaacagttt acctggcgtg gtggtgttaa ctacctgttt gataatggcg
    11821 taacacctta ctttagctat agcgaatcgt ttgaaccttc ttcgcaagtt gggaaggatg
    11881 gtaatatttt cgcaccgtct aaaggtaagc agtatgaagt cggcgtgaaa tatgtaccgg
    11941 aagatcgtcc gattgtagtt actggtgccg tgtataatct cactaaaacc aacaacctga
    12001 tggcggaccc tgagggttcc ttcttctcgg ttgaaggtgg cgagatccgc gcacgtggcg
    12061 tagaaatcga agcgaaagcg gcgctgtcgg cgagtgttaa cgtagtcggt tcttatactt
    12121 acaccgatgc ggaatacacc accgatacta cctataaagg caatacgcct gcacaggtgc
    12181 caaaacacat ggcttcgctg tgggctgact ataccttctt tgacggtccg ctttcaggtc
    12241 tgacgctggg caccggtggt cgttatactg gctccagcta tggtgatccg gctaactcct
    12301 ttaaagtggg aagttatacg gtcgtggatg cgttagtgcg ttatgatctg gcgcgagtcg
    12361 gcatggcggg ctccaacgtg gcgctgcatg ttaacaacct gttcgatcgt gaatacgtcg
    12421 ccagctgctt taacacttat ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg
    12481 caaccttccg tttctaattt ctcttttggg gcacggattt ccgtgcctgt ttcacaagtt
    12541 ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc gtaatatctc
    12601 ctttcgtgtg cccgggcgca cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa
    12661 agtgaccggt ctgattggtc acaacggttc tggtaaatcc actctgctca aaatgcttgg
    12721 ccgtcatcag ccgccgtcgg aaggggagat tcttcttgat gcccagccgc tggaaagctg
    12781 gagcagcaaa gcgttcgccc gcaaagtggc ttatttgccg cagcagcttc ctccggcaga
    12841 agggatgacc gtgcgtgaac tggtggcgat tggtcgttac ccgtggcatg gcgcactagg
    12901 gcgctttggt gcggcagatc gcgaaaaagt tgaggaagct atctcgctgg ttggcttaaa
    12961 accgctggcg catcggctgg tagacagcct ctctggcggc gaacgtcagc gggcgtggat
    13021 cgccatgctg gtggcgcagg atagccgttg tctgttgctc gacgaaccga cttcggcgtt
    13081 ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc aggagcgtgg
    13141 cctgacggtc attgccgtgc tgcacgatat caatatggct gcgcgttact gtgattatct
    13201 ggtcgccctg cgcggcggtg aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg
    13261 cgaaaccctc gaaatgattt atggcatccc gatgggtatt ctgccgcatc cggcgggtgc
    13321 tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg ccgtcgactg
    13381 ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca cgcggcggct
    13441 attgacccaa atcgtattgt ggcgctggag tggctgccgg tggaattact actggcgctc
    13501 ggcatcgtgc cttatggcgt ggcagacacc atcaactatc gactgtgggt cagcgaacca
    13561 ccactacctg aatcggtgat tgacgtcggt ttgcgtacag aacctaacct cgaactgctg
    13621 accgaaatga aaccgtcgtt tatggtctgg tcggcgggat atggtccttc gccagaaatg
    13681 ctggctcgta ttgcgccggg gcgtggattt aacttcagcg acggcaaaca gccgctggcg
    13741 atggcgcgta aatcgctgac ggaaatggcg gatttactta acctgcaaag cgcagcagaa
    13801 acgcatttag cgcaatatga agactttatc cgcagcatga acccccgctt tgtgaagcgt
    13861 ggtgcgcgtc cgttattgct gacgacgctt atcgatccgc gccatatgct ggtcttcggt
    13921 ccaaacagct tgttccagga aattcttgat gagtacggca tcccaaatgc ctggcaaggg
    13981 gaaaccaact tctggggcag taccgccgtc agcatcgatc gtctggcagc gtataaagac
    14041 gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct aatggcaacg
    14101 ccgctgtggc aggccatgcc gtttgtccgc gccggacgct ttcagcgcgt acccgcagtc
    14161 tggttttatg gtgcaactct ctcggcaatg cactttgtgc gcgttctgga taacgccatt
    14221 ggaggtaaag cgtgagtaaa cgtattgcgc ttttcccggc attattgctg gcgttgctgg
    14281 tgattgccgc tacggcgctg acctggatga acttctcgca ggcgctgccg cgtagccagt
    14341 gggcgcaggc tgcctggtcg ccggatattg acgtcatcga gcagatgatt tttcactaca
    14401 gcttgctgcc gcgtctggcg atttcgctgc tggtgggcgc aggcctgggg ctggtgggcg
    14461 tgctgtttca gcaagtgctg cgtaatccgc tggcggaacc gacgacactt ggcgttgcta
    14521 caggcgcgca actggggatt accgtcacta cgctctgggc gatcccaggg gcgatggcga
    14581 gtcagtttgc tgcgctggcg ggcgcttgcg ttgttggctt aatcgtcttt ggcgtcgcgt
    14641 gggggaaacg gctttcgccg gtaacgctga tcctcgcggg gctggtagtg agcctttatt
    14701 gtggggcaat caatcagtta ctggttatct tccaccatga ccaactgcaa agcatgttcc
    14761 tgtggagcac cggaacgctg acgcaaaccg actggggcgg tgttgagcgt ttatggccgc
    14821 agttgctggg cggtgtgatg ctgacgttgc tgctacttcg tccgttaaca ctgatggggc
    14881 ttgatgatgg cgtggcgcgc aatctcgggc tagccttgtc gctggcgcgt ctggcggcgc
    14941 tgtcgctggc tattgtcatc agtgcgctgc tggtgaacgc ggtggggatt atcggcttta
    15001 tcgggttgtt cgcgccgttg ctggcaaaaa tgctgggggc gcggcgtctg ctgccgcgac
    15061 tgatgctggc gtcgttgatt ggtgcgctga tcctctggct ttccgatcaa atcatcctct
    15121 ggctgactcg cgtgtggatg gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc
    15181 cgctgctact gtggctgttg ccgcgtttac gcagcattag cgcgccggat atgaaggtca
    15241 acgatcgtgt cgcggctgaa cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc
    15301 tgctgttgat ggctgtggtg gtggcgctgt cgtttggtcg tgatgcgcac ggctggacgt
    15361 gggcgagcgg ggcgttgctc gaggatttga tgccctggcg ctggccgcga attatggcgg
    15421 cgctgtttgc gggcgtcatg ctggcggtgg cgggctgtat tattcagcgg ctgaccggaa
    15481 acccgatggc aagcccggaa gtgctgggga ttagctctgg cgcggcgttt ggcgtggtac
    15541 tgatgctgtt tctggtaccg ggtaatgctt ttggctggct gttacccgcg ggaagtatcg
    15601 gtgcggcggt gacgctgttg attattatga tcgccgccgg gcgcggtgga ttttccccac
    15661 accgcatgtt actggcgggg atggcgttaa gtaccgcatt caccatgctt ttaatgatgt
    15721 tgcaggcgag tggtgacccg cgaatggcgc aagtgctgac ctggatctct ggttcgacct
    15781 acaacgccac cgatgcgcag gtctggcgca ccggaattgt gatggtgatt ttgctggcga
    15841 ttaccccgct gtgccgccgc tggctgacca ttttaccgct gggtggtgat accgcgcggg
    15901 ccgtgggggt ggcgttaacg ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga
    15961 cggcgaccgc gacgatgacc attggaccgt tgagttttgt tggtttaatg gcaccgcata
    16021 tcgcgcggat gatgggcttt cgacggacga tgccgcacat cgtgatttcg gcgctggtgg
    16081 gtggtttact gctggtgttc gctgactggt gtgggcggat ggtgctgttt ccgttccaga
    16141 tcccggcggg gctgctgtcg acctttatcg gcgcgccata ttttatctat ttgttgagaa
    16201 agcagagccg ttaataaaat gcctcgcccg gtgggcgagg caaaatcatc acaacttcgc
    16261 aaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt gcgccacaga
    16321 cataaagccc gcttcaaacg ctgacggtgc caggtaaaca ccttcatcca gcatcatatg
    16381 gaagaaacgc ttaaaacgct cgacgtcaca ggccatcaca tcctgatagc acgtcacgga
    16441 ctcggcgtcg gtaaagaaaa taccgaacat gccgccaacg tggtttacca ccagcggaat
    16501 tccggcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca gctcatccag
    16561 cgtttcgtga acgcccggct gcgcgacttc attcagacag gcgaaaccgg ctgccatcgc
    16621 aatcgggtta ccggaaagcg tacccgcctg atagaccgga cccgtcgggg ccagcgcatc
    16681 cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga tgattttgcc
    16741 caggcaagtg agatccggtt ccacaccgta ataatcctgt gcgccagcta gcgctacgcg
    16801 gaaaccggtc attacttcat cgatgatcag caacgcgcca aattcgtcgc acagcgcgcg
    16861 cagacctggc aggaagtctg gcagcggtgg aacacagttc atattgcctg ccaccggctc
    16921 gacgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta cagaagccag
    16981 atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacgc ccggcgagtt
    17041 tggctggcct aacgtgagtg cgccagagcc ggctttcacc agcaggcagt cagcgtgacc
    17101 gtggtaacaa ccttcaaatt taataatttt gtcgcgaccg gtaaaaccac gggccaggcg
    17161 gatggcgctc attgtcgctt cagtgccgga gttcaccatg cgcaccatat ccatggtcgg
    17221 gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggttggtg caccaaagct
    17281 taaaccacgc tcggcggctt caatcacggc attgcggatc gccggatggt tatggcccag
    17341 caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa catcgtacag
    17401 ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag taaaggcgcg
    17461 aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa gattttcaga
    17521 cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt ctatgttatt
    17581 cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa tcctgatttc
    17641 ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat tttcatggaa
    17701 cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat tggatgaaaa
    17761 ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga caactgattc
    17821 gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg
    17881 gcacgcttgt cgggctggca gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc
    17941 aacgtatggg ggcgctggta catactgctg ataattatcc gctactgtta accgttgctt
    18001 ttctctgttc ggcggtgctg gcgatgttcg gctacttttt ggtgcgcaaa tacgcgcccg
    18061 aagcaggggg gtcggggatc ccggaaattg aaggggcgct ggaagatcaa cgtcctgttc
    18121 gctggtggcg tgtattgccg gtgaaattct ttggcgggct ggggacactc ggcggaggca
    18181 tggtgttggg gcgcgaaggg ccaaccgtgc agatcggcgg taatatcggg cggatggtac
    18241 tcgatgtttt ccgcctgaaa ggtgatgaag ctcgccatac gctgttggca accggtgcgg
    18301 cggcgggtct ggcggcggcc tttaacgcgc cgctggcggg tattttgttt attatcgaag
    18361 agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt attggcgtca
    18421 ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt gacgtcggta
    18481 aactttctga cgcgccgctt aatacgctgt ggctttatct gatcctcggt attatttttg
    18541 gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg ctgcaccgtg
    18601 tgcacggcgg caatattacc aaatgggtac tgatgggtgg tgcgattggc ggtctgtgtg
    18661 gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg attcctatcg
    18721 ctaccgcggg gaatttcagc atgggaatgc tggtgtttat cttcgtcgcg cgggtcatta
    18781 ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg atgctggcgc
    18841 tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt ccgcaatatc
    18901 accttgaggc ggggacgttt gctattgccg gaatgggggc gttactggcg gcatctattc
    18961 gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac cagctcattt
    19021 tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc ggcgggaaac
    19081 cgctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc
    19141 gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt attagataat
    19201 ggcgattatt attgggttag aatttgccca attgccgatg tcgtttggag caaaatatga
    19261 gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa gttaaaagcc
    19321 tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcacc ggtggcggtt
    19381 gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc gatatgacca
    19441 tcgaaaaaca gggcgttggc ctggtggttg atccgatgag cctgcaatat ctggtcggcg
    19501 gttccgttga ctataccgaa ggactggaag gttctcgttt catcgtgacc aacccgaacg
    19561 cgaaaagcac ctgcggttgc ggttcttcct ttagtatcta atctgttgtt catgtgccgg
    19621 ataaactatc cggcacatct tctctcttca acgcccattc tcatccagcg caaacgtcgg
    19681 tagcttaaga tgccaacgaa tagccgccag ccgaatcaat agcgtcacga ccatgcccat
    19741 catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc
    19801 gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga tttcgcgggc
    19861 cagaacatca cgaatgatcc cgccgccaac gccagtaatg acgcccatac aaaccgcgat
    19921 taacggaccg gcttccgcat taaaggcttt attcacgcca atgccgacaa acaccgccag
    19981 accaacggcg tccagcaccg gcaacatcca ttttggtaag tgtcttggct ggcgcaccag
    20041 cacgatggtc agcatgctgg ttaccattgc aacgaccaga tcggtgggat ctttcaccca
    20101 aaataccggg ccgtgatcca gcgccatgtc gcgaattgtc ccgccgccta cagcggtaac
    20161 cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta acaaaacgcc
    20221 ggagatggca aataccgctg tgccgactat atccagccaa tagacgagca ttgttcaatc
    20281 cccactgagc acctaaaaag gtcaggcgct aatctacctg tgaaagcgca ttacagagct
    20341 gttgtgcagc gaggataata cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa
    20401 taacgggaat tttgagctgt tcaccccagt attgtttgat tttaggaatt tggtccggtc
    20461 cgcctgtaat gacaatcgcc tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg
    20521 gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg agtacctggt
    20581 tttgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc agaaaaacac
    20641 gttttttagg tttatcagca tattgcgctt tcaattgcgc gtattgatcc agcagggatt
    20701 gcgcggcttg ttcggcctta tccggttgcg gactccaggg agccagttga cgtaacgcat
    20761 tggcaatttg ttcaatgttt gtcgcatcga cccacatcac ttttattccc agcgaagcca
    20821 gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga tcgggtttca
    20881 gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aacctgctca atcttttgcg
    20941 cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg
    21001 caagttcagt gttggcggga gaaagcgtga tgacgcgcgg cgcggcgttg agccacagtg
    21061 gcgcaagaaa agacagggcg accagcgccc tgaacagtga cttagccatg tgccagtttc
    21121 tgcactagtg actcaaccat caggctggac tgtttagcgg caacagccag gaactcatcg
    21181 aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac aacaaacggg
    21241 acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac agcaatggcc
    21301 tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg
    21361 ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc ctcagcggca
    21421 gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg accgtattca
    21481 taaccaaatg ccgtgacatc cgcgtcgtga taacgcgctt cgtccgagac aacgatatcg
    21541 cccactttca gcgttggtgc caggccaccg gcagaaccgg tgttaataat cacatctggc
    21601 ttgcagtgtt ccaacagcag agtggcaccc agcgccgcag cgactttacc gatgcccgat
    21661 ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca accgcccaga
    21721 ctgatagttt gacggttttc gattttgtca cgcagcagcg taacttcttc ttccattgca
    21781 ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaag gcatagttta
    21841 ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac agattgattt
    21901 ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca
    21961 tgagatcctg cggatcttcg agagcgatcg cgggcgtatc atcaactctc cggcaattcg
    22021 tcgtctgcaa caaaagaccc aggtttttcc actggagcgc aatgccgccg tgcgcacgcg
    22081 tcttacccac tcgatggaag tccagcaggt ggggcgctac atcgccaaag aaattttaag
    22141 ccgtctgaaa gagcttaaat tgctggaagc atacggcctg gatgaactga ccggaccttt
    22201 tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc cgtttggtca
    22261 ttttggcgaa gcggcgataa atgactggtt tcgccagcgt ttgcacccgg aagatgccga
    22321 aagccagcct ctgactgacg atcgctgcag cgtggcggta ctacgtttac gggacgggga
    22381 agaaccgctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa
    22441 tgcacaaggc attcgtctgg tgcatacatt gatgcggatg aatctcacct gggcgcaggt
    22501 tggcggtatt ttaaaatata cccgtccagc gtggtggcgt ggcgaaacgc ctgagacaca
    22561 tcactattta atgaaaaagc cgggttatta tctttctgaa gaagcctata ttgcccggtt
    22621 gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacgtgga ttatggaagc
    22681 tgccgacgac atctcctatt gtgtggcaga tcttgaagat gcggtagaga aaagaatatt
    22741 taccgttgag cagctttatc atcatttgca cgaggcgtgg ggccagcatg agaaaggttc
    22801 gctcttttcg ctggtggttg aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg
    22861 cagtacggaa gatcagtttt ttatgtattt acgggtaaac accctaaata aactggtacc
    22921 ctacgcggca caacgattta ttgataatct gcctgcgatt ttcgccggaa cgtttaatca
    22981 tgcattattg gaagatgcca gcgaatgcag cgatcttctt aagctatata aaaatgtcgc
    23041 tgtaaaacat gtgtttagcc atccagatgt cgagcagctt gaattgcagg gctatcgggt
    23101 cattagcgga ttattagaga tttatcgccc tttattaagc ctgtcattat cagactttac
    23161 tgaactggta gaaaaagaac gggtgaaacg ttcccctatt gaatctcgct tattccacaa
    23221 actctcgacg cgccatcggc tggcctatgt cgaggctgtc agtaaattac cgtcagattc
    23281 tcctgagttt ccgctatggg aatattatta ccgttgccgc ctgctgcagg attatatcag
    23341 cggtatgacc gacctctatg cctgggatga ataccgacgt ctgatggccg tagaacaata
    23401 accaggcttt tgtaaagacg aacaataaat ttttaccttt tgcagaaact ttagttcgga
    23461 acttcgcgct ataaaacgaa tctgaagaac acagcaattt tgcgttatct gttaatcgag
    23521 actgaaatac atgaaaaaaa ccacattagc actgagtgca ctggctctga gtttaggttt
    23581 ggcgttatct ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca
    23641 gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt
    23701 agaaggtagc acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg
    23761 tgatgattct ccgttctgcc aggaaggttc tccgttccag agctctccgt tctgccaggg
    23821 tggccagggc ggtaatggtg gcggccagca acagaaattc atggcgctgg gttccggcgt
    23881 catcattgat gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac
    23941 ggtcattaaa gttcaactga gcgatggccg taagttcgac gcgaagatgg ttggcaaaga
    24001 tccgcgctct gatatcgcgc tgatccaaat ccagaatccg aaaaacctga ccgcaattaa
    24061 gatggcggat tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt
    24121 tggtctgggc gagacggtaa cttccgggat tgtctctgcg ctggggcgta gcggcctgaa
    24181 tgccgaaaac tacgaaaact tcatccagac cgatgcagcg atcaaccgtg gtaactccgg
    24241 tggtgcgctg gttaacctga acggcgaact gatcggtatc aacaccgcga tcctagcacc
    24301 ggacggcggt aacatcggta tcggttttgc tatcccgagt aacatggtga aaaacctgac
    24361 ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga
    24421 gctgaactcc gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag
    24481 ccaggttctg cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac
    24541 ctcactgaac ggtaagctga tcagcagctt tgccgcactg cgtgctcaag tgggtactat
    24601 gccggtgggc agcaaactga ccctgggctt gctgcgcgac ggtaagcagg tcaacgtgaa
    24661 tctggaactg cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat
    24721 tgaaggcgct gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa
    24781 aacgggcact ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggcgcgaa
    24841 ccagcaggct gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt
    24901 gctggcactg aacattcagc gcggcgacag caccatctac ctgttaatgc agtaatctcc
    24961 ctcaacccct tcctgaaaac gggaaggggg tctccttaca atctgtgaac ttcaccacaa
    25021 ctccatacat cttcatcatc ctttaggcat ttgcacaatg ccgtacgtta cgtacttcct
    25081 tatgctaagc cgtgcataac ggaggactta tggctggctg gcatcttgat accaaaatgg
    25141 cgcaggatat cgtggcacgt accatgcgca tcatcgatac caatatcaac gtaatggatg
    25201 cccgtgggcg aattatcggc agcggcgatc gtgagcgtat tggtgaattg cacgaaggtg
    25261 cattgctggt actttcacag ggacgagtcg tcgatatcga tgacgcggtg gcacgtcatc
    25321 tgcacggtgt gcggcaaggg attaatctac cgttacggct ggaaggtgaa attgtcggcg
    25381 taattggcct gacaggtgaa ccagagaatc tgcgtaaata tggcgaactg gtctgcatga
    25441 cggctgaaat gatgctggaa cagtcgcggt tgatgcactt gttggcgcag gatagccgtt
    25501 tgcgggagga actggtgatg aacctgattc aggcagagga gaatactccc gcacttactg
    25561 aatgggcgca acggctgggg atcgatctca atcaaccgcg agtggtggct attgttgagg
    25621 tcgacagcgg tcagcttggt gtggacagcg caatggcgga gttacaacaa ctgcaaaacg
    25681 cgctgactac gcccgagcgt aataatctgg tggcgattgt ctcgctaacc gaaatggtgg
    25741 tgttgaaacc ggcgttgaac tcttttgggc gctgggatgt agaagatcat cgtaagcgag
    25801 ttgaacaact gattacccgc atgaaagagt acggccagct gcgttttcgc gtttcactgg
    25861 gcaactattt taccggtcct ggcagtattg cccgatccta tcgtacggcg aaaacgacga
    25921 tggtggtggg taaacagcgg atgccagaaa gtcgctgcta tttttatcag gatctgatgt
    25981 tacctgtgtt actcgacagt ttgcgtggcg actggcaggc caacgaactg gcgcgaccgc
    26041 tggcgcggct gaaagcgatg gacaataacg gcttgctgcg acgaacgctg gcggcgtggt
    26101 ttcgtcacaa tgtgcaaccg ctggcaacgt caaaggcgtt gtttattcat cgtaataccc
    26161 tggagtatcg gcttaatcgt atatcggaac tgaccgggct tgatttgggc aattttgatg
    26221 acaggttgct gctgtatgtg gcgttacaac tggatgaaga gcggtaggtt atgcgttaag
    26281 gttgaggcgg cgctggctca ttcccctcag cccgaagggg agaggggaat gcccgagtta
    26341 accgtcaatt cgggattatt tacgcgttag tttttccaga tcggcttcaa tctcgctgat
    26401 cttattggtt acgactgact ccaggtgacg taagtcgtcg aggatcttac gcttaagatc
    26461 aacttcgctg cggtcacgct ggcagatttg atcaagctca tcaatgatat accgtagatt
    26521 cgggctgatt tcctggactt ctttataacc ctgacccaca ccatcagcga cgaccgtttt
    26581 acgctgacgc ggatatttaa acttaacgct cttggcgaaa aactcgcctt tgtctttctg
    26641 gaaatagatt ttcagaatat cgttgttggc ttcctgccgg aggctgtaac gatcaatttc
    26701 ttcaggattg gtaataccca gacttttcag attgtcgtac atagcgttac ctcaaaatga
    26761 gtcagtaaaa tgtgcttatt ttagcatttg gcctggcccg cccggctcga ctgtgatcgc
    26821 agaaagcctt tttcttccag acttctatca tggcgtaaaa atcaaaaaat tacctgcttt
    26881 attgtggcga tattccatgg gatggattag cacatttatc ttcgttttgc gaattacatg
    26941 acagtccgaa acaagatgaa aaagtattta aaaaaggggg gaggaagaat gagtaggacg
    27001 aattattctg aaccagaaag caatcgggca gcgtcgtgct gcccgtgtgc atacttttag
    27061 tcgatggtac gcagcagttc gttaatgccg actttgccgc gagttttcgc gtcaactttc
    27121 ttaacgataa ccgcacagta gaggctgtat ttgccatctt tcgacggcag attacctgaa
    27181 acaaccacag accccgccgg aacgcgaccg tagtggatgt cgccggtttc acggtcgtaa
    27241 atacgggtgc tctgaccaat gtatacgccc atggaaatga cggaaccttc ttcgacaatc
    27301 accccttcaa ctacttcaga gcgcgcgccg atgaagcaat tatcttcaat gatggttggg
    27361 ttagcctgca gcggttccag cacaccgccg atgcccacgc caccggaaag gtggacgttt
    27421 ttaccaatct gcgcacaaga accgacggtc gcccaggtat cgaccatggt gccttcatca
    27481 acatatgcgc cgatgttgac gtaagacggc atcagcacgg tgttacgggc aataaacgca
    27541 ccctgacgta ccgccgcagg tggtacaacg cggaagcctt ctttctggaa acgtgcttcg
    27601 tcgtagtcgg cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc
    27661 acctgattat cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc
    27721 acccactgac cgtcaatttt ttccgctaca cgcagtgcgc cggaatccag cagggcgatc
    27781 acctgattta ccgcttcgcg ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg
    27841 cgttcaaaag cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat
    27901 cagtaaacac atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact
    27961 tcctgctgca actcgttatt aagtgcacgc cggtcagcgg tggcaattat gaataaatct
    28021 tctactcgct cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca
    28081 aaaattttcc cgactctcgc cagcagtcct ggctggtcga gcgcgataag ctcaaggaac
    28141 gatttgcggt cggtatgggt tggcaaaaac gtcacttcgg tttcaacagt aaagtgacgt
    28201 agtttagccg gttgacgacg cggctgtggt ggctgccagc tactttgtgt cagtacttgc
    28261 tccagaccaa accgaataac ctcatgacga tctgcggata gcgggctacc atcgggttcc
    28321 agcacgataa aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga
    28381 acgcttaagt tgcgtctatc taactcggca caaacagcag caaacaagta agggcggtcc
    28441 gggctccaga taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag caataccagc
    28501 ggtttgctta aatcatgctg taataaatgg cgcgcatgcc aggccagttg atttgggcta
    28561 tgacggacaa aatagttcgc gcgacagcgt gaccaaattt ggtgcagcac ctcttcgtcg
    28621 atgttatcca tgcgcagtag tgccagcgcc tggagttggt gatggcgaac ccgttcgcgc
    28681 atatccggcg tgttttgcat tccgcgtcgt agctgctttt cggtggcgaa atagagttcg
    28741 cgcaacagac tttgcttcca gctattccac agcgtttcgt tggtggcgca aatgtcagcc
    28801 actgtcaggc ataccagata gcgcagacga ttttccgttt gaacttcttc ggcaaactgc
    28861 ttgatgactt ccgggtcctg aatatcgcga cgctgagctg tcaccgacat cagcaagtgc
    28921 tggcgaacca gccaggcgac cagctgtgtt tcgcgtgagt tcagcccgtg gagttcggca
    28981 aaatgcacta catcctgagc accgagaata gagtggtcgc cgccgcgtcc tttggcgata
    29041 tcgtgaaaca gcgcggcgat gaaaatcagc tcagttgacg gtaggcgcgg ccagacgttc
    29101 acacacaacg gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcagcatc
    29161 acgcggatag tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg
    29221 acgatatgtg accattgcgg catatacgcg ccgagtacgc tatggcgatg cattggcaat
    29281 agcccgcgcc gcaccgctcc ggggtgacgt agaatgctca aaaacagttt tcgtgcttgc
    29341 ggaatattac acagcggttg ttgcagatgg cgacgggcat ggcgtaactg gcgcagtgtg
    29401 gtggagtaaa tgccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg
    29461 gcttccggct ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt accgcgtagc
    29521 tgaaactcat cgtcgattgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca
    29581 tcgaacagtt gcagcagcat ctggttgagt tcactgacgc ggcgtgtcac gcggaagtaa
    29641 tccttcatca tccgctcgac cggttcgtta ccttcgccac tgtaattcag acgctgggcg
    29701 acgctaagct ggcgatcgaa taacaggcga ttatcgtaac ggctgacgac cagatgcagg
    29761 gcaaagcgaa tacgccacaa tatatgcaga cattcgttta attccgcccg ctccgctgag
    29821 gttaagaagc caaacccaac catttcatcc agcgatgttg cgccaaaatg acggcgggcc
    29881 acccattgca gagtgtggat atcgcgcaag ccgccagggc tacttttgat gtctggttca
    29941 agattgtagc tggtgccgtg gtaacgctga tggcgctggt tctgttcttc aactttcgcc
    30001 gcgtagaact tgtcggaagg ccagaatcct tcgctgaaaa tatgtttttg cagttcaaga
    30061 aatagcgcaa catcgccaat taataagcgg gattcgatca aattggtggc gacggttaaa
    30121 tccgataacc cttccagcat gcactcttca agcgtgcgca cactatgacc gacttccagc
    30181 tttacatccc agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc
    30241 tttttacggc ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg
    30301 tagccaccga cggcgaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat
    30361 aatcgttgca ggagctggtc gataaactcg gtgcgcgcct caatcaactg ttctgcagag
    30421 atcccattgt caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc
    30481 ccaccgacgg ttaattcatc acggggccag acgcatggat tttgcggttg accgggcagg
    30541 gtggggagag cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa
    30601 gattaaatta tcgcattaaa aaagccggca ttcgccggct tcattttatt cgtcgtgcga
    30661 gattatcgcc gggatggtgt catccttgcg tagcgtcaga atttcgcagc cgttatcagt
    30721 caccacaata gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca
    30781 gccatctttc atggtgcgga tctctttttt acccgcgttg accattggct cgatggtgaa
    30841 cgtcatccca ggtttcagta cgacgttggt ttcacgggag tcatagtgca gcacctgcgg
    30901 ttcttcatgg aagccgcgac caataccgtg tccgcaatat tcacgaacga cggagaagcc
    30961 ttctgcttcg acaaatttct gaatcgccgc accgatttcg cgcagattaa tgcctggttt
    31021 taccatgcgt agcgccaggt acaggctttc ttgcgtgata cggcacagac gttcgcccat
    31081 gatggtcggc ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat
    31141 tacggtgaca tcaatgttaa cgatatcgcc atctttcagc agcttagcat cgtccggaat
    31201 accgtggcac accacttcat taatagaaat gcaaacggat ttcggataac cgtgatagcc
    31261 gaggcaggct gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag
    31321 ctcgccggtg ctgacgcccg gtttaacata tggttcgatc atctccagca cttcggcagc
    31381 cagtcggcca gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat
    31441 taattctgtc catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta
    31501 tcacacccgg gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaaggtttt
    31561 ctataataga aaattcgacg tctgatgctg tacacagcgc caacaattat tggtgtccac
    31621 ggcgtatttg tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga
    31681 cggaagcgac aatctcactt tgtgtaacaa cacacacgta tcggcacata ttccggggtg
    31741 ccctttgggg tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt
    31801 tttaatcatg gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca
    31861 ccagacccgt tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt
    31921 tcacatcatc aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa
    31981 caagattgct tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga
    32041 agcggtgaaa gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg
    32101 cggtatgctg actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga
    32161 aactcagtct caggacggta ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac
    32221 tcgtgagctg gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc
    32281 ggacgctctg tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa
    32341 cctgggtatt ccggtatttg ctatcgttga taccaactct gatccggacg gtgttgactt
    32401 cgttatcccg ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc
    32461 tgcaaccgta cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt
    32521 agaagctgag taataaggct tgataactcc cccaaaatag ttcgagttgc agaaaggcgg
    32581 caagctcgag aattcccggg agcttacatc agtaagtgac cgggatgagc gagcgaagat
    32641 gacgcatctg cggcgcgaaa tatgaagggg gagagccctt atagaccagg tagtacacgt
    32701 ttggttaggg ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc
    32761 cgggcagatc acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa
    32821 gagctgcgtg agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct
    32881 aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg
    32941 aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac
    33001 ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag
    33061 gcgttcgcag acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt
    33121 ctgaaagcac agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc
    33181 aacattcgcc gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcatggtgcg
    33241 cgtatcggcg ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct
    33301 atgcacgttg ctgcaagcaa gccagaattc atcaaaccgg aagacgtatc cgctgaagtg
    33361 gtagaaaaag aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa
    33421 atcgcagaga aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc
    33481 ggtcagccgt tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac
    33541 gctgaagtga ctggcttcat ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact
    33601 gactttgcag cagaagttgc tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc
    33661 ctgagggcgg cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg
    33721 ctgaaaagcg acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg
    33781 aaagaaacat ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg
    33841 gcgaagctct gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgtatgg
    33901 ctcaggaaat caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg
    33961 gtaacctgtt ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc
    34021 acatggggat gctggcgacc gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg
    34081 cctatgtgaa cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca
    34141 gctgggcaga agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta
    34201 caggtaaccc gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag
    34261 ccgatgtggt gctgaaagca accaaagttg acggcgtgtt taccgctgat ccggcgaaag
    34321 atccaaccgc aaccatgtac gagcaactga cttacagcga agtgctggaa aaagagctga
    34381 aagtcatgga cctggcggcc ttcacgctgg ctcgtgacca taaattaccg attcgtgttt
    34441 tcaacatgaa caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt
    34501 taatcacgga ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg
    34561 ggggatctta cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt
    34621 gactgtctgg tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag
    34681 aaaagatgct gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa
    34741 aatacgcacg ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg
    34801 cacgccgacg ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa
    34861 aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcgtccga
    34921 tcttggcctg aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac
    34981 ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt
    35041 tgcagtacgt aacgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa
    35101 agagatcagc gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc
    35161 tgcaatcaag aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg
    35221 atttcttgaa cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt
    35281 gctttttatt ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga
    35341 ccggctcgat tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg
    35401 tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct
    35461 ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac
    35521 agcaacaggg tagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag
    35581 cgcttgagga tgttgatcag gtgatggcag ccattgttgg cgctgctggg ctgttaccta
    35641 cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta
    35701 cctgcggacg tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg
    35761 atagcgaaca taacgccatt tttcagagtt taccgcaacc tatccagcat aatctgggat
    35821 acgctgacct tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt
    35881 tccgtgagac gccattgcgc gatttggcaa caatgacgcc cgatcaagcc tgccgtcatc
    35941 cgaactggtc gatgggacgt aaaatttctg tcgattcggc taccatgatg aacaaaggtc
    36001 tggaatacat tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga
    36061 ttcacccgca gtcagtgatt cactcaatgg tgcgctatca ggacggcagt gttctggcgc
    36121 agctggggga accggatatg cgtacgccaa ttgcccacac catggcatgg ccgaatcgcg
    36181 tgaactctgg cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac
    36241 cggattatga tcgttatcca tgcctgaaac tggcgatgga ggcgttcgaa caaggccagg
    36301 cagcgacgac agcattgaat gccgcaaacg aaatcaccgt tgctgctttt cttgcgcaac
    36361 aaatccgctt tacggatatc gctgcgttga atttatccgt actggaaaaa atggatatgc
    36421 gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc gagcgcgcgt gaagtcgcca
    36481 gaaaagaggt gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt
    36541 tgttagcgta gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta
    36601 taaggtcagg tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg
    36661 agtaccgtaa accctgtcag ggaataaaaa acgcgtgatg ttgtctgcta ctcaaccact
    36721 tagcgaaaaa ttgccagcgc atggctgccg tcatgttgcg atcattatgg acggcaatgg
    36781 tcgctgggca aaaaagcaag ggaagattcg tgcctttggg cataaagccg gagcaaaatc
    36841 cgtccgccgg gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc
    36901 ctttagtagt gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt
    36961 tgtgtgggcg ctcgatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat
    37021 tattggcgat accagtcgct ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc
    37081 gctaacagcc gggaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg
    37141 ggatatagtc cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc
    37201 agatcagata gatgaagaga tgctaaacca gcatgtctgt atgcatgaac tggcccctgt
    37261 agatttagta attaggactg ggggggagca tcgcattagt aactttttgc tttggcaaat
    37321 tgcctatgcc gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt
    37381 tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg
    37441 tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt
    37501 gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt
    37561 aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac
    37621 tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt
    37681 tctgttgccg gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc
    37741 ttcgctgggt tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc
    37801 aatctggcgt aactctaaaa cattgcgcct tatttttggc gtgctaacca ttgttccctt
    37861 cttctggggc atgctggcgt tacgggcctg gcactatgac gagaatcatt acagtggcgc
    37921 aatatggctg ctctatgtca tgatcctggt atggggcgct gactccggcg catatatgtt
    37981 tggcaaattg tttggtaaac ataagctggc accgaaggtt tctccgggta aaacctggca
    38041 aggctttatc ggtggactcg ctactgcagc ggtaatctca tggggttatg gcatgtgggc
    38101 gaatctcgac gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc
    38161 agtgctcggc gatctgaccg agagtatgtt taagcgtgaa gcaggaatta aggacagcgg
    38221 tcatttaatt ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt
    38281 accggtcttt gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg
    38341 ctgagttttc tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg
    38401 catgaatttg gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca
    38461 atagggtttg gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc
    38521 gccctgatcc cgttgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt
    38581 ccggaactcc gccaccatgc cttcaataat aaatctgtcg gccaacgagc ggcgattatt
    38641 gccgcaggtc cggttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt
    38701 attggtgtgc ctggcgtacg tccggtggtt ggcgaaatag cagccaattc gatagctgcg
    38761 gaagcacaaa ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat
    38821 tgggatgccg tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattaca
    38881 gtagcgccat ttggcagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg
    38941 tttgagcctg ataaagaaga tccggtatct tcgctgggga ttcgtcctcg tgggccgcaa
    39001 attgaacctg tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa
    39061 gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg
    39121 atgcttgtcc gggataaccc gggtaaatcc ttagcgttag aaatcgaaag gcaggggagt
    39181 cccttgtctt tgacattaat cccggagagt aaaccgggta atggtaaagc gattggtttt
    39241 gtcggtattg agccgaaagt cattcctttg ccagatgagt ataaagttgt acgccagtat
    39301 gggccgttca acgccatcgt cgaagccacg gacaaaacgt ggcagctgat gaagctgacg
    39361 gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg
    39421 atctctatcg ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg
    39481 tttcttgcgc ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt
    39541 gacggggggc atctgctgtt ccttgcgatc gaaaagatca aaggcggacc ggtatccgag
    39601 cgggttcaag acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt
    39661 gcacttttca atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga
    39721 tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg
    39781 ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg
    39841 cggccctcct cagtatgccg gtgcgcacag gcgacacggt taatgatgaa gatatcagta
    39901 ataccattcg cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg
    39961 gtgataccct tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg
    40021 gtaacaaatc ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg
    40081 tgggcgaatc cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct
    40141 actacagcgt cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca
    40201 accgtgttga cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta
    40261 acattgttgg taaccatgct ttcaccaccg acgaactgat ctctcatttc caactgcgtg
    40321 acgaagtgcc gtggtggaac gtggtaggcg atcgtaaata ccagaaacag aaactggcgg
    40381 gcgaccttga aaccctgcgc agctactatc tggaccgcgg ttatgcccgt ttcaacatag
    40441 actctaccca ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca
    40501 ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag cggcaacctt gccgggcact
    40561 ccgctgaaat tgagcagctg actaagatcg agccgggcga gctgtataac ggcaccaaag
    40621 tgaccaaaat ggaagatgac attaaaaagc ttctcggtcg ctatggttat gcctatccgc
    40681 gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg
    40741 ttgatgcggg taaccgtttc tacgtgcgta agatccgttt tgaaggtaac gatacctcga
    40801 aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg tgcatggctg ggaagcgatc
    40861 tggtcgatca gggtaaggag cgtctgaatc gtctgggctt ctttgaaact gtcgataccg
    40921 atacccaacg tgttccgggt agcccggacc aggtagatgt agtctacaag gtgaaagaac
    40981 gtaacactgg tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc
    41041 aggctggcgt gcaacaggat aactggttag gtacaggtta tgctgttggt atcaacggga
    41101 ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag
    41161 atggcgtaag cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc
    41221 tgtccgacta taccaacaag agttatggta cagacgtgac gttgggcttc ccgattaacg
    41281 aatataactc gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc
    41341 ctcaggttgc gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc
    41401 aggataacag cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc
    41461 ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca
    41521 ttcctggatc ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga
    41581 tcgatgacga tcacaaatgg gttgttctgg ggcgtacccg ctggggttat ggtgatggtt
    41641 taggcggcaa agagatgccg ttctacgaga acttctatgc cggtggttcc agcaccgtgc
    41701 gtggcttcca gtccaataca attggtccga aagcagttta cttcccgcat caggccagta
    41761 attatgatcc ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gacctgtgta
    41821 aatcggatga tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc
    41881 cgacgccgtt tattagcgat aagtatgcta actcggttcg tacttccttc ttctgggata
    41941 tgggtaccgt ttgggataca aactgggatt ccagccaata ttctggatat ccggactata
    42001 gtgatccaag caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg
    42061 ggccgttggt gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac
    42121 agttccagtt taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta
    42181 gtgtagcgat gactttaggc gatcaatata aatcgccggg ccacgcaaag aactgcaccc
    42241 tccggtgcaa atgggatggt aaggagttta ttgtgaaaaa gtggttatta gctgcaggtc
    42301 tcggtttagc actggcaaca tctgctcagg cggctgacaa aattgcaatc gtcaacatgg
    42361 gcagcctgtt ccagcaggta gcgcagaaaa ccggtgtttc taacacgctg gaaaatgagt
    42421 tcaaaggccg tgccagcgaa ctgcagcgta tggaaaccga tctgcaggct aaaatgaaaa
    42481 agctgcagtc catgaaagcg ggcagcgatc gcactaagct ggaaaaagac gtgatggctc
    42541 agcgccagac ttttgctcag aaagcgcagg cttttgagca ggatcgcgca cgtcgttcca
    42601 atgaagaacg cggcaaactg gttactcgta tccagactgc tgtgaaatcc gttgccaaca
    42661 gccaggatat cgatctggtt gttgatgcaa acgccgttgc ttacaacagc agcgatgtaa
    42721 aagacatcac tgccgacgta ctgaaacagg ttaaataagt aatgccttca attcgactgg
    42781 ctgatttagc gcagcagttg gatgcagaac tacacggtga tggcgatatc gtcatcaccg
    42841 gcgttgcgtc catgcaatct gcacaaacag gtcacattac gttcatggtt aacccaaaat
    42901 accgtgagca tttaggcttg tgccaggcgt ccgcggttgt catgacccag gacgatcttc
    42961 ctttcgcgaa aagtgccgcg ctggtagtga agaatcccta cctgacttac gcgcgcatgg
    43021 cgcaaatttt agataccacg ccgcagcccg cgcagaacat tgcacccagt gcggtgatcg
    43081 acgcgacggc gaagctgggt aacaacgtat cgattggcgc taacgcggtg attgaatccg
    43141 gcgttgaact gggcgataac gtgattatcg gtgccggttg cttcgtaggt aaaaacagca
    43201 aaatcggtgc aggttcgcgt ctctgggcga acgtaaccat ttaccatgag atccagatcg
    43261 gtcagaattg cctgatccag tccggaacag tggtaggcgc agacggcttt ggttatgcca
    43321 acgatcgtgg taactgggtg aagatcccac agattggtcg cgtaattatt ggcgatcgcg
    43381 tggagatcgg tgcctgcact accatcgatc gcggcgcgct ggatgacact gttattggca
    43441 atggcgtgat cattgataac cagtgccaga ttgcacataa cgtcgtgatt ggcgacaata
    43501 cggcggttgc cggtggcgtc attatggcgg gcagcctgaa aattggtcgt tactgcatga
    43561 tcggcggagc cagcgtaatc aacgggcata tggaaatatg cgacaaagtg acggttacgg
    43621 gcatgggtat ggtgatgcgt cccatcactg aaccaggcgt ctattcctca ggcattccgc
    43681 tgcaacccaa caaagtctgg cgcaaaaccg ctgcactggt gatgaacatt gatgacatga
    43741 gcaagcgtct gaaatcgctt gagcgcaagg ttaatcaaca agactaacgt tccatctttt
    43801 gttcgccaaa ctttacggcc tgtctcattc ttacgattgc ggcaggccgt gttattattg
    43861 tcgtttctta tattttgaca ggaagagtat cttgactact aacactcata ctctgcagat
    43921 tgaagagatt ttagaacttc tgccgcaccg tttcccgttc ttactggtgg atcgcgtgct
    43981 ggattttgaa gaaggtcgtt ttctgcgcgc agtaaaaaat gtctctgtca atgagccatt
    44041 cttccagggc catttccctg gaaaaccgat tttcccgggt gtgctgattc tggaagcaat
    44101 ggcacaggca acaggtattc tggcgtttaa aagcgtagga aaactggaac cgggtgagct
    44161 gtactacttc gctggtattg acgaagcgcg cttcaagcgc ccggtcgtgc ctggcgatca
    44221 aatgatcatg gaagtcactt tcgaaaaaac gcgccgcggc ctgacccgtt ttaaaggggt
    44281 tgctctggtc gacggtaaag tagtttgcga agcaacgatg atgtgtgctc gtagccggga
    44341 ggcctgatac gtgattgata aatccgcctt tgtgcatcca accgccattg tggaagaggg
    44401 cgcgtcgatt ggcgcgaacg cacacattgg tcctttttgt atcgttggac cccatgtcga
    44461 aattggtgag ggtaccgtac tgaaatctca cgttgtcgtg aatggtcata ctaaaattgg
    44521 ccgcgataat gagatttatc agttcgcctc catcggcgaa gttaaccagg atctgaaata
    44581 tgctggcgaa ccgacccgcg tggaaatcgg cgatcgtaac cgcattcgcg aaagcgtcac
    44641 cattcatcgt ggcacagtcc agggcggtgg attgacgaag gtgggcagcg acaacttact
    44701 gatgatcaac gcgcacattg cgcacgattg tacggtaggt aaccgctgta ttctcgccaa
    44761 caacgcaacg ctggcgggtc acgtatcggt tgacgacttc gcgatcatcg gcggcatggc
    44821 cgcagtccat cagttctgca tcattggtgc gcatgtgatg gttggcggct gctccggtgt
    44881 ggcgcaggac gtccctcctt atgtcattgc gcagggtaac cacgccacgc cgttcggtgt
    44941 caatatcgaa gggctgaagc gccgcggatt cagccgtgag gcgattaccg ctatccgcaa
    45001 tgcgtataag ctgatttatc gtagcggtaa aacgctcgat gaagtgaaac cggaaattgc
    45061 tgaactggcg gaaacatatc cggaagtgaa agcctttacc gatttctttg cacgctcaac
    45121 gcgcggtctg attcgttaat gactgaacag cgtccattaa cgattgccct ggtcgccgga
    45181 gaaacctccg gcgatatcct gggggccggt ttaatccgcg ctctgaaaga acgtgtgccc
    45241 aacgcccgct ttgttggtgt tgccgggcca cgaatgcagg ctgaaggctg cgaagcctgg
    45301 tacgaaatgg aagagttggc ggtgatgggc attgttgaag tgctcggtcg tctgcgtcgc
    45361 ttactgcata ttcgtgccga tctgacaaag cgttttggtg aactaaagcc agatgttttt
    45421 gttggcattg atgcgcctga cttcaatatt atcctcgaag gtaaccttaa aaagcagggt
    45481 atcaaaacca ttcattatgt cagtccgtcc gtctgggcgt ggcgacagaa acgcgttttc
    45541 aaaataggca gagccaccga tctggtgctc gcatttctgc ctttcgaaaa agcgttttat
    45601 gacaaataca acgtaccgtg ccgctttatc ggtcatacca tggctgatgc catgccatta
    45661 gatccagata aaaatagtgc ccgtgatgtg ctggggatcc cttacgatgc ccactgtctg
    45721 gcattgttgc cgggcagccg tggtgcagaa gtcgaaatgc ttagtgccga tttcctgaaa
    45781 acggcccagc ttttgcgcca gacatatccg gatctcgaaa tcgtggtgcc actggtgaat
    45841 gccaaacgcc gcgagcagtt tgaacgcatc aaagctgcag tcgcgccaga cctgtcggtt
    45901 catttgctgg atggaatggg ccgtgaggcg atggtcgcca gcgatgcggc actactggcg
    45961 tcggggacgg cagccctgga gtgtatgctg gcgaaatgcc cgatggtggt gggatatcgc
    46021 atgaagcctt ttaccttctg gttggcgaag cggctggtga aaactgatta tgtctcgctg
    46081 ccaaatctgc tggcgggcag agagttagtc aaagagttat tgcaggaaga gtgtgagccg
    46141 caaaaactgg ctgcggcgct gttaccgctg ttggcgaatg ggaaaaccag ccacgcgatg
    46201 cacgatacct tccgtgaact gcatcagcag atccgctgca atgccgatga gcaggcggca
    46261 caagccgttc tggagttagc acaatgatcg aatttgtcta tccgcacacg cagctggttg
    46321 cgggtgtgga tgaagtcgga cgcgggccgt tagttggcgc ggtcgtcacc gctgcggtga
    46381 ttctcgaccc ggcgcgcccg attgccgggc tgaatgattc caaaaagctg agcgaaaaac
    46441 gtcgtctggc gctctgtgaa gagatcaaag agaaagcgtt gagctggagt ctgggccgcg
    46501 cggaacctca cgaaatcgac gaactgaata ttttacatgc gaccatgctg gcgatgcagc
    46561 gagccgtcgc tgggctgcat attgcgccgg aatatgtgtt gattgatggt aaccgctgcc
    46621 cgaaattacc gatgcctgcg atggctgtgg tgaaaggcga tagccgcgta ccggaaatca
    46681 gtgccgcgtc tatcctggcg aaagtgacgc gtgacgccga aatggcggcg ctggatattg
    46741 ttttcccgca atatggtttt gcccaacaca aagggtatcc aaccgctttt catctggaaa
    46801 aactggctga acacggcgcg accgaacacc atcggcgcag ctttgggcct gtcaaacgcg
    46861 cactgggact tgcgtcctga ttcttgtgtc gagattaagt aaaccggaat ctgaagatgt
    46921 ctgaaccacg tttcgtacac ctgcgggtgc acagcgacta ctcgatgatc gatggcctgg
    46981 ccaaaaccgc accgctggta aaaaaggcgg cggcgttggg tatgccagca ctggcgatca
    47041 ccgatttcac caacctttgc ggtctggtga agttctacgg agcgggacat ggcgcaggga
    47101 ttaagcctat cgtcggggca gattttaacg tccagtgcga cctgctgggt gatgagttaa
    47161 cccacctgac ggtactggcg gcgaacaata ccggctatca gaatctgacg ttgctgatct
    47221 caaaagcgta tcagcgcggg tacggtgccg ccgggccgat catcgatcgc gactggctta
    47281 tcgaattaaa cgaagggttg atccttcttt ccggcgggcg catgggcgac gtcggacgca
    47341 gtcttttgcg tggtaacagc gcgctggtag atgagtgtgt cgcgttttat gaagaacact
    47401 tcccggatcg ctattttctc gagctgatcc gcaccggcag gccggatgaa gaaagctatc
    47461 tgcacgcggc ggtggaactg gcggaagcgc gcggtttgcc cgtcgtggcg actaacgatg
    47521 tgcgctttat cgacagcagc gactttgacg cacacgaaat ccgcgtcgcg atccacgacg
    47581 gctttaccct cgacgatcct aaacgcccgc gtaactattc gccgcagcaa tatatgcgta
    47641 gcgaagagga gatgtgcgag ctgtttgccg acatcccaga agcccttgcc aacaccgttg
    47701 agatcgccaa acgctgtaac gtaactgtgc gtcttggtga atacttcctg ccgcagttcc
    47761 cgaccgggga catgagcacc gaagattatc tggtcaagcg tgcaaaagag ggcctggaag
    47821 agcgtctggc ctttttattc cctgatgagg aagaacgtgt taagcgccgc ccggaatatg
    47881 acgaacgtct ggagactgaa cttcaggtta tcaaccagat gggcttcccg ggctacttcc
    47941 tcatcgttat ggaatttatc cagtggtcga aagataacgg cgtaccggta gggccaggcc
    48001 gtggctccgg tgcgggttca ctggtggcct acgcgctgaa aatcaccgac ctcgatccgc
    48061 tggaatttga cctgctgttc gaacgtttcc ttaacccgga acgtgtctcc atgcctgact
    48121 tcgacgttga cttctgtatg gagaaacgcg atcaggttat cgaacacgta gcggacatgt
    48181 acggtcgtga tgcggtatcg cagattatca ccttcggtac gatggcggcg aaagcggtga
    48241 tccgcgacgt aggccgcgtg ctggggcatc cgtacggctt tgtcgatcgt atctcgaaac
    48301 tgatcccgcc cgatccgggg atgacgctgg cgaaagcgtt tgaagccgag ccgcagctgc
    48361 cggaaatcta cgaagcggat gaagaagtta aggcgttgat cgacatggcg cgcaaactgg
    48421 aaggggtcac ccgtaacgcc ggtaagcacg ccggtggggt ggttatcgcg ccgaccaaaa
    48481 ttaccgattt tgcgccgctt tattgcgatg aagagggcaa acatccggtc acccagtttg
    48541 ataaaagcga cgttgaatac gccggactgg tgaagttcga cttccttggt ttgcgtacgc
    48601 tcaccatcat caactgggcg ctggagatga tcaacaagcg gcgggcgaag aatggcgagc
    48661 cgccgctgga tatcgccgcg atcccgctgg atgacaagaa aagcttcgac atgctgcaac
    48721 gctcggaaac cacggcggta ttccagcttg aatcgcgcgg catgaaggac ctgatcaagc
    48781 gtctgcaacc tgactgcttc gaagatatga tcgcactggt ggcactgttc cgccctggtc
    48841 cgttgcaatc agggatggtg gataacttta tcgaccgtaa acatggtcgc gaagagatct
    48901 cctatccgga cgtgcagtgg cagcatgaaa gcctgaaacc ggtactggag ccaacctacg
    48961 gcattatcct gtatcaggaa caggtcatgc agattgcgca ggtgctttct ggttataccc
    49021 tcggtggcgc ggatatgctg cgtcgtgcga tgggtaagaa aaagccggaa gagatggcta
    49081 agcaacgttc tgtatttgct gaaggtgcag aaaagaacgg aatcaacgcc gaactggcga
    49141 tgaaaatctt cgacctggtg gagaaattcg ctggttacgg atttaacaaa tcgcactctg
    49201 cggcctatgc tttggtgtca tatcaaacat tatggttgaa agcgcactat ccggcagagt
    49261 ttatggcggc ggtaatgacc gccgatatgg acaacaccga gaaggtggtg ggcctggtgg
    49321 atgagtgctg gcggatgggg ctgaaaatcc tgccaccaga tataaactcc ggtctttacc
    49381 atttccacgt caacgacgac ggcgaaatcg tgtatggtat tggcgcgatc aaaggggtag
    49441 gtgaaggtcc gattgaggcg atcatcgaag cccgtaataa aggcggctac ttccgcgaac
    49501 tgtttgatct ctgcgcccga accgacacca aaaagttgaa ccgtcgcgtg ctggaaaaac
    49561 tgatcatgtc cggggcgttt gaccgtcttg ggccacatcg cgcggcgctg atgaactcgc
    49621 tgggcgatgc gttaaaagcg gcagatcaac acgcgaaagc ggaagctatc ggtcaggccg
    49681 atatgttcgg cgtgctggcc gaagagccgg aacaaattga acaatcctat gccagctgcc
    49741 aaccgtggcc ggagcaggtg gtattagatg gggaacgtga aacgttaggc ctgtacctga
    49801 ccggacaccc tatcaaccag tatttaaaag agattgagcg ttatgtcgga ggcgtaaggc
    49861 tgaaagacat gcacccgaca gaacgtggta aagtcatcac ggctgcgggg ctcgttgttg
    49921 ccgcgcgggt tatggtcacc aagcgcggca atcgtatcgg tatctgcacg ctggatgacc
    49981 gttccgggcg gctggaagtg atgttgttta ctgacgccct ggataaatac cagcatttgc
    50041 tggaaaaaga ccgcatactt atcgtcagcg gacaggtcag ctttgatgac ttcagcggtg
    50101 ggcttaaaat gaccgctcgc gaagtgatgg atattgacga agcccgggaa aaatatgctc
    50161 gcgggcttgc tatctcgctg acggacaggc aaattgatga ccagctttta aaccgactcc
    50221 gtcagtctct ggaaccccac cgctctggga caattccagt acatctctac tatcagaggg
    50281 cggatgcacg cgcgcggttg cgttttggcg cgacgtggcg tgtctctccg agcgatcgtt
    50341 tattaaacga tctccgtggc ctcattggtt cggagcaggt ggaactggag tttgactaat
    50401 acaggaatac tatgagtctg aatttccttg attttgaaca gccgattgca gagctggaag
    50461 cgaaaatcga ttctctgact gcggttagcc gtcaggatga gaaactggat attaacatcg
    50521 atgaagaagt gcatcgtctg cgtgaaaaaa gcgtagaact gacgcgtaaa atcttcgccg
    50581 atctcggtgc atggcagatt gcgcaactgg cacgccatcc acagcgtcct tataccctgg
    50641 attacgttcg cctggcattt gatgaatttg acgaactggc tggcgaccgc gcgtatgcag
    50701 acgataaagc tatcgtcggt ggtatcgccc gtctcgatgg tcgtccggtg atgatcattg
    50761 gtcatcaaaa aggtcgtgaa accaaagaaa aaattcgccg taactttggt atgccagcgc
    50821 cagaaggtta ccgcaaagca ctgcgtctga tgcaaatggc tgaacgcttt aagatgccta
    50881 tcatcacctt tatcgacacc ccgggggctt atccgggcgt gggcgcagaa gaacgtggtc
    50941 agtctgaagc cattgcacgc aacctgcgtg aaatgtctcg cctcggcgta ccggtagttt
    51001 gtacggttat cggtgaaggt ggttctggcg gtgcgctggc gattggcgtg ggcgataaag
    51061 tgaatatgct gcaatacagc acctattccg ttatctcgcc ggaaggttgt gcgtccattc
    51121 tgtggaagag cgccgacaaa gcgccgctgg cggctgaagc gatgggtatc atcgctccgc
    51181 gtctgaaaga gctgaaactg atcgactcca tcatcccgga accgctgggc ggcgctcacc
    51241 gtaacccgga agcgatggcg gcatcgttga aagcgcaact gctggcggat ctggccgatc
    51301 tcgacgtgtt aagcactgaa gatttaaaaa atcgtcgtta tcagcgcctg atgagctacg
    51361 gttacgcgta attcgcaaaa gttctgaaaa agggtcactt cggtggccct tttttatcgc
    51421 cacggtttga gcaggctatg attaaggaag gattttccag gaggaacaca tgaacatcat
    51481 tgccattatg ggaccgcatg gcgtctttta taaagatgag cccatcaaag aactggagtc
    51541 ggcgctggtg gcgcaaggct ttcagattat ctggccacaa aacagcgttg atttgctgaa
    51601 atttatcgaa cataaccctc gaatttgcgg cgtgattttt gactgggatg aatacagtct
    51661 cgatttatgt agcgatatca atcagcttaa tgaatatctc ccgctttatg ccttcatcaa
    51721 cacccactcg acgatggatg tcagcgtgca ggatatgcgg atggcgctct gtttttttga
    51781 atatgcgctg gggcaggcgg aagatatcgc cattcgtatg cgtcagtaca ccgacgaata
    51841 tcttgataac attaccccac cgttcacgaa agccttgttt acctacgtca aagagcggaa
    51901 gtacaccttt tgtacgccgg gtcatatggg cggcaccgca tatcaaaaaa gcccggttgg
    51961 ctgtctgttt tatgactttt tcggcgggaa tacccttaag gctgatgtct ctatttcggt
    52021 caccgagctt ggttcgttgc tcgaccacac cgggccacac ctggaagcgg aagagtacat
    52081 cgcgcggact ttcggcgcgg aacagagtta tatcgttacc aacggaacat cgacgtcgaa
    52141 caaaattgtg ggtatgtacg ccgcgccatc cggcagtacg ctgttgattg accgcaattg
    52201 tcataaatcg ctggcgcatc tgttgatgat gaacgatgta gtgccagtct ggctgaaacc
    52261 gacgcgtaat gcgttgggga ttctgggtgg gatcccgcgc cgtgaattta ctcgcgacag
    52321 catcgaagag aaagtcgctg ccaccacgca ggcacaatgg ccggttcatg cggtgattac
    52381 caactcaacc tatgatggct tgctctacaa caccgactgg atcaaacaga cgctggatgt
    52441 cccgtcgatc cacttcgatt ccgcctgggt gccgtacacc cattttcatc cgatctacca
    52501 ggggaaaagt ggtatgagcg gcgagcgtgt tgcgggaaaa gtgatcttcg aaacgcaatc
    52561 gacccacaaa atgctggcgg cgttatcgca ggcttcgctg atccacatta aaggtgagta
    52621 tgacgaagag gcgtttaacg aagcctttat gatgcatacc accacctcgc ccagttatcc
    52681 cattgttgct tcggttgaga cggcagcagc gatgctgcgt ggtaatccgg gcaaacggct
    52741 gattaaccgt tcagtagaac gagctctgca ttttcgcaaa gaggtccagc ggctgcggga
    52801 agagtctgac ggttggtttt tcgatatctg gcaaccgccg caggtggatg aagccgaatg
    52861 ctggcccgtt gcgcctggcg aacaatggca cggctttaac gatgcggatg ccgatcatat
    52921 gttcctcgat ccggttaaag tcactattct gacgccgggg atggacgagc agggcaatat
    52981 gagcgaggag gggatcccgg cggcgctggt ggcgaagttc ctcgatgagc gtgggatcgt
    53041 ggtagagaaa accggcccat ataacctgct gtttctcttt agtattggca tcgataaaac
    53101 caaagcaatg ggattattgc gtgggttgac ggaatttaaa cgctcttacg atctcaatct
    53161 gcgaatcaaa aatatgcttc cggatctgta cgcagaagat cccgatttct accgcaatat
    53221 gcgtattcag gatctggcgc aagggatcca taagctgatt cgtaaacacg atcttcccgg
    53281 tttgatgttg cgggcattcg atactttgcc ggagatgatc atgacgccac atcaggcatg
    53341 gcagagacag attaaaggtg aagtagaaac cattgcgctg gaacaactgg tcggtagagt
    53401 atcggcaaat atgatcctgc cttatccacc gggcgtaccg ctgttgatgc ctggagaaat
    53461 gctgaccaaa gagagccgca cagtactcga ttttctactg atgctttgtt ccgtagggca
    53521 acattacccc ggttttgaaa cggatattca cggtgcgaaa caggacgaag acggcgttta
    53581 ccgcgtacga gtcctaaaaa tggcgggata acttgccaga gcggcttccg ggcgagtaac
    53641 gtgctgttaa caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg
    53701 attattgcga cggattatgc ggtgagcaaa gctttctact gcgatattct tggtttcacg
    53761 ctgcaaagcg aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat
    53821 gggcaatatg tgattgagct tttctcattt ccgttcccgc cggagcgccc cagccgaccg
    53881 gaagcttgcg gcctgcgtca tctggctttt agcgttgatg acatcgatgc ggcagtggcg
    53941 caccttgaaa gccataacgt gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa
    54001 cgcttcacct tctttaacga tccggacggg ctgccgttgg aactgtatga gcagtaaggc
    54061 ttgtcatcgc cgcatttgcc cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat
    54121 catgacactc acgctcaata gacaacttct cacctcacgc cagattctgg tggcctttag
    54181 cggcgggctt gactccaccg ttctgctgca tcagttggtg cagtggcgga cggaaaatcc
    54241 ggggggcgct ctgcgcgcta ttcatgtgca tcacggttta agtgccaatg ccgatgcctg
    54301 ggttacgcat tgcgaaaacg tctgccaaca gtggcaggtg ccgctggtgg tcgaacgcgt
    54361 acaacttgcg caagaggggc tggggattga ggcccaggcg cggcaggcgc gttatcaggc
    54421 atttgcccgc accttgttgc ccggtgaagt gctggtcacc gcgcaacatc tcgacgatca
    54481 atgtgaaact tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat
    54541 ggcggaagtt tcggagtttg ccggaacacg gcttatccgc ccgttgcttg cccgcacgcg
    54601 gggggaactg gcgcagtggg cattggcgca tggtttacgc tggattgaag acgaaagtaa
    54661 tcaggacgac tcatacgatc gtaactttct gcgcctgcgc gtagtgccgt tattgcagca
    54721 gcgttggccg cattttgccg aaacaacggc ccgcagcgcc gcactttgtg ctgaacaaga
    54781 gagcctgctg gatgaactgc tggcagatga tttagcacac tgtcaatcgc cgcaggggac
    54841 gctgcagatt gtgccaatgc tggcgatgag tgatgcccgc cgcgcggcga ttatccgccg
    54901 ctggctggca gggcagaatg caccgatgcc ttcccgcgac gcgttggtga ggatctggca
    54961 ggaagtggcg ctggcgcggg aagatgcctc cccctgttta cgtttgggcg cgtttgaaat
    55021 ccgacgctat cagtcgcaac tgtggtggat taaatccgtc accgggcaaa gcgaaaacat
    55081 tgtgccgtgg cagacatggc ttcaaccgct ggaattaccg gcggggcagg gaagtgtaca
    55141 gcttaatgcg ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt
    55201 caaagcgcca ggattgctgc atattgtcgg gcgtaacggc ggacgtaagc taaagaaaat
    55261 ctggcaagag ctgggcgtgc cgccgtggct acgtgacacc acgccactgc tgttttatgg
    55321 cgaaacgctg attgcggcgg cagggggatt tgtgacgcaa gaaggtgtgg ctgaaggtga
    55381 gaatggcata agttttgtct ggcagcgaaa cgcttagttt agtgaaggcc ggatatgacg
    55441 cattaagcgt cgcatccggc gaaagtcaat caggactcgc tcaccaccac cgtaccgatt
    55501 tccgggtggc taaagctggt aattttatcc agacgtagct cacgggtttc gccagcggcc
    55561 tcgacgacca gatactccac atttttgcgg gagactaaat cgctggcttt ggcctgcaat
    55621 ttttcgccat ctttcagctc aagtgtcagc attaaatgat gctggcaggc gagctcaaga
    55681 ttatcgtaat catcacaatt gattggttga tacgtatcat tcattgacat aatcgctcac
    55741 cagtaagttt gccgcagcgt atgctgcttt ttccctgaca gcctcagaaa gggcgtcgtc
    55801 ggcagccatt tcattcagca ctttcaaaac gcagcccagc gcgtccggaa cgtatcctaa
    55861 gtctccgctg gcgatttccg cgtaccgctt gcgtattaac tcacaatatt tttccacatg
    55921 ccctcctgtc agcactctga cttaaccgtg gatgcaagtc taagcctacg aagataaact
    55981 ctgtttcgca aggtgactat accacactca tttctgcaat atcagcgccg caactgcacg
    56041 tattccgtta caatggcctc ctgattcgaa aggagttttc ttatggcgct taaagcgaca
    56101 atttataaag cgacggttaa tgtggccgat ctcgaccgca accagtttct cgatgcctct
    56161 ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta tgatgctgcg cctgctggcg
    56221 tggctgaaat atgctgatga acgtctgcaa tttacccgtg gtttgtgtgc cgatgatgag
    56281 ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt tgtggattga gctggggctg
    56341 ccggatgagc gacgaattaa gaaagcctgc acccaggccg cagaagtggc gctgtttacc
    56401 tataatagtc gggcggcgca aatctggtgg cagcaaaatc agagcaaatg tgcgcagttt
    56461 gccaatcttt ccgtctggta tctggacgac gagcagctgg cgaaagtgag cgcctttgcc
    56521 gatcgtacca tgacgctgca ggcaacgatt caggatggcg tgatctggtt atcggatgat
    56581 aagaataatc tggaagtgaa cttaaccgcc tggcaacaac cttcatgatt gtgatttccc
    56641 gacatgttgc tattcccgat ggtgagcttg agatcaccgc cattcgtgcg cagggcgcgg
    56701 gcgggcagca tgttaataag acctcaacgg ccattcatct gcgttttgac attcgggcat
    56761 ccagcctgcc agagtattac aaagagcgtc tgctcgccgc cagccatcat ttgatcagca
    56821 gtgatggcgt aattgtcatt aaggcacagg aataccgcag tcaggaactg aaccgcgaag
    56881 cggcgctggc ccggctggtg gcagtgatta aagatttaac aacagaacaa aaagcccgac
    56941 gacccacgcg gcccacccgt gcatcgaaag agcgcaggct ggcatcgaaa gcacaaaaat
    57001 caagcgtgaa ggcgatgcgc agcggtcggg aataaaaaga aggaatggat ggtgaaaaaa
    57061 gcgatagtga cagcgatggc tgtaatcagc ctctttactc tgatgggttg taataatcgg
    57121 gccgaagtcg atacgctttc tccggcgcag gctgccgaat tgaaaccgat gccgcaaagt
    57181 tggcgcggcg tgctgccgtg tgccgattgc gaaggaatcg aaacctctct gttcctcgaa
    57241 aaagacggaa catgggtgat gaatgagcgt tatctcggtg ctcgtgaaga accttcctcc
    57301 ttcgcttcct acggtacatg ggcgcgaacc gctgacaagc tggtattaac cgacagcaaa
    57361 ggtgaaaagt catattatcg ggcgaaaggc gatgcgctgg agatgctcga tcgtgaaggc
    57421 aatccgattg aatcgcagtt caactatacg ctggaaccgg cacaatccag tttacccatg
    57481 acgccgatga ccctgcgggg catgtatttt tatatggctg atgcggcgac cttcactgat
    57541 tgcgcgaccg gaaaacgttt catggtagcg aataacgcag agctggagcg tggctacctg
    57601 gctgcgcgcg gccacagtga aaaaccggtg ttactgtcag tagaaggtca ctttacgctt
    57661 gaggctaatc cggataccgg tgcgccaacc aaagtattag cacccgatac ggcagggaaa
    57721 ttttacccaa accaggattg cagtagtttg gggcagtaac ccgtcttgag acagaaacaa
    57781 acgcaaaaca ggccagaagg atatatttca acattttgaa tttgcacgtt ttttgtaggc
    57841 cggataaggc gtttacgccg catccggcaa tggtgctcaa cgcctgatgc gacgctggcg
    57901 cgtcttatca tgcctacaac ccccctcata ccttaaggcg gataaggcat ttactttatc
    57961 accgggtcaa accaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa
    58021 catactgtaa ctgcgtttct ggcttaaacg cagagacatc accaatacgc atatgcatca
    58081 gatcggcagg gctaatccag cccgattgcg ccagcgtaag cggatgcccg gctttggcaa
    58141 atgcatcagt gacaaattcc gaacaaaacc acgacttttt gtctccttcg accacactgc
    58201 ttaactgcgc tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat
    58261 tcagtgagca catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt
    58321 tataaccgct atctttgatt ttattggcga acgcggtgat ttctgtggct tgttgcgggg
    58381 taagatccgg gactcgtaag acgaaaagtt tatcactatg cttcatcgct tttttaaggg
    58441 aaacaatctg gacgccagcg cctgtcgctt ctgcaacgtt attttcaccc agatagattg
    58501 caacgtgact cacagaggaa gtgctgaaaa cgcggatccc aaatgaggtt acctccaggc
    58561 ttgaggagaa cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt
    58621 gttcggtgaa agagctttga tgctggaatt tgacagccca tgttttcgcc tcggcatcta
    58681 ctgctgtggc tgatgagtct ggctggctga tatcaacggt acaggccgaa agtaaaagaa
    58741 agcaggggag aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta
    58801 aaaaagggcc ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga
    58861 tattcgacga tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat
    58921 tcgatatcgt cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc
    58981 agttccatat cggcaaacat cacgcccgga cgttcttttc ggtcatccag cagcacttcg
    59041 ataccttgtg cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat
    59101 ttgtgcatgt tcatcggcag aattgccacc tggaacggcg cgatagcgtc aggccatacg
    59161 atgccgcgct cgtcgtagtt ctgctcaatc gccgcagcca ctacgcgcgt taccccgata
    59221 ccgtagcaac ccatagtcag gatttggtta cggccatctt caccctgtac ggaggctttc
    59281 agtgcttcgg agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg
    59341 atcagcagcg taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatcc
    59401 gcaacttccg gggtagcgac atcgcgatcc cagttgatgc cgaagtagtg tttaccatcg
    59461 atgttagcac cagcagtgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga
    59521 atcggcatgt ttaccggacc cagtgaaccc ggaccggctt taaccacggc acgaatttct
    59581 tcttcggtcg cgaaagtcag cgggctggca acctgcggca gtttttctgc tttaacttcg
    59641 ttcagctcgt gatcaccgcg caccagcagc gcaaccagcg ggaagctact gccttcaacc
    59701 gctttaacca gcagagtctt aaccgttttc ttaatcggca gattgaactg ttcaaccagt
    59761 tccgcgatgg ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg
    59821 cgcggttctt tcggcgcgat agcttctgcc agctcaatgt tcgctgcata gtcagaggtg
    59881 tcggagaaga ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg
    59941 ctgccgccga tagaaccggt gtcggcctgt acggcgcgga aatccagccc catgcggctg
    60001 aagattttgc tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa
    60061 gtatggaaag agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc
    60121 ggacgcactt cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg
    60181 taagagctaa gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt
    60241 acgaacggac gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa
    60301 cgaccactct cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca
    60361 ccggcgttgt tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg
    60421 gtcggcagcc aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc
    60481 agctgatggc tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat
    60541 tggctagtac gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc
    60601 tgggacaaaa aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt
    60661 gaatttaacg cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa
    60721 cgttaaaatc atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg
    60781 ccgggcgcgg gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct
    60841 tctccagcgt caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag
    60901 ctggcgcact ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat
    60961 acggtttgat atccactacc ggcgtaccat cgaccagatc caggctgccg agcttcagaa
    61021 tcacgcagtc tttatggcaa acaacctctt tcagctctac cagcgacata ccaatcgggt
    61081 tggggcggaa agtggagcgc gtagcgaaaa cacccattct ggcattaccg ccaagacgag
    61141 gcggacgcac agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc
    61201 ataaatggct gaatgcttcc aggccgcgaa cggcgtcagc ctggttgtag ggagcaatga
    61261 gatgtagttc gccgttggcg ctttttacca gacctggctg gcgcggaacg gcgaattttt
    61321 ctttataggg cgagcgaata acacctattt gctcgaactg gaaactgctc atttcgccgt
    61381 aatgttaagc gcagaaccga tacataaagc ctgacgatag cagcctggcg taccgctggt
    61441 aacttcgcag ctatgcagta ataccgcatt ggctttcatt ttcgaggcgt tgatttgcat
    61501 ccgcttacgc gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc
    61561 gccactgact tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata
    61621 aattcggacc ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg
    61681 tgcagtgctt tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat
    61741 gagtgctact aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac
    61801 gtcaatattg aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg
    61861 atattacaaa ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa
    61921 cactttgttt gctgcttcgt aaacttcgtc agactgataa gcctggacga atttcttcac
    61981 gttctcggcg tctttgttat cttcacgcgt tacgatcagg tttacgtacg gggagtcttt
    62041 atcttcgaca aagataccgt ttttcgctgg agtcaggcca atctggctgg cataggtggt
    62101 gttaataact gccagagcga tttgcgcgtc gtccagagaa cgcggcagtt gcggtgcttc
    62161 cagttcaaca attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag
    62221 gccaacgcca tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc
    62281 aaggttagtt ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga
    62341 tttgattttc ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag
    62401 tttgtagcca cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc
    62461 gtcgatatcg cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag
    62521 ctcaacgtcc aggccatatt tgtctttcgc aactttctgc gcgacttctg caacctgctg
    62581 ttcggcacca acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca
    62641 gcctaccagt gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa
    62701 cgccatacct tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac
    62761 agcccggacg atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac
    62821 cagtaccgta ttcatcaccg tcgcgttata gccgatgtag ccatactgat agccaatctg
    62881 acctaaacca ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag
    62941 ggtgatagtt gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac
    63001 gatctgcatt ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc
    63061 cagcagagcg ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac
    63121 aattgctgcc tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca
    63181 taccagcaag ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg
    63241 atacagcttc gcgttagcaa taatttgccc cggacgcgtg acataaagca ggacgccaac
    63301 cggcagacca accacaaacc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca
    63361 tacgccacga accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta
    63421 catggtgttc ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt
    63481 cagtcagcat gatgccgaac ttaacgccac cggcgtaatc catctgcgcg ctaataatgt
    63541 tgttgttgac gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac
    63601 cggtaaactc cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt
    63661 cctggtaatc ttccgggata tccagatgca gggttgactg aataaacttc tgcgccagcg
    63721 gcgttttcgg atgtgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc
    63781 tgatgaccgc aacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca
    63841 gaatcgtcaa acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg
    63901 ttgccgggtc tagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta
    63961 atgcacgggc aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat
    64021 catgcttatc tccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt
    64081 ctttcggtgt attgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag
    64141 agagcaggtt aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg
    64201 attctgacag cgtggtcagt tcctgtccat cgaccagcac gctaccctcg gttgggcgct
    64261 ccagcaggtt tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc
    64321 cataaatttg tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg
    64381 tgccctggtg gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt
    64441 cattaagtta gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga
    64501 tgttaaggca tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg
    64561 tcgaaacatg cgatactagc gtcacatgcc ttattaagga gctataaaag gtggcgaaga
    64621 gcgtacccgc aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc
    64681 atgagatcga caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa
    64741 aaatgggctt tgcgctggtg gtagtcacca accagtctgg cattgctcgc ggtaaattta
    64801 ccgaagcaca gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg
    64861 tcgatctgga tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc
    64921 gccaggtctg cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt
    64981 tgcatattga tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag
    65041 cggttgcggc gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg
    65101 aagcagaaaa cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa
    65161 aaaagcagca aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt
    65221 ttccgcttgt cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcgaatgtg
    65281 aatcacttca cacaaacagc cggttcggtt gaagagaaaa aat
//
LOCUS       DABSYT020000015        65019 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.015, whole genome shotgun sequence.
ACCESSION   DABSYT020000015 DABSYT020000000
VERSION     DABSYT020000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.015"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            39..671
                     /gene="comR"
                     /locus_tag="ID725_RS06570"
                     /old_locus_tag="pgaptmp_001314"
     CDS             39..671
                     /gene="comR"
                     /locus_tag="ID725_RS06570"
                     /old_locus_tag="pgaptmp_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGV7849952.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(733..1272)
                     /gene="ycfJ"
                     /locus_tag="ID725_RS06575"
                     /old_locus_tag="pgaptmp_001315"
     CDS             complement(733..1272)
                     /gene="ycfJ"
                     /locus_tag="ID725_RS06575"
                     /old_locus_tag="pgaptmp_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGV7849953.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1499..2803)
                     /gene="ndh"
                     /locus_tag="ID725_RS06580"
                     /old_locus_tag="pgaptmp_001316"
     CDS             complement(1499..2803)
                     /gene="ndh"
                     /locus_tag="ID725_RS06580"
                     /old_locus_tag="pgaptmp_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGV7849954.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3203..3745)
                     /gene="ycfP"
                     /locus_tag="ID725_RS06585"
                     /old_locus_tag="pgaptmp_001317"
     CDS             complement(3203..3745)
                     /gene="ycfP"
                     /locus_tag="ID725_RS06585"
                     /old_locus_tag="pgaptmp_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGV7849955.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3768..4793)
                     /gene="nagZ"
                     /locus_tag="ID725_RS06590"
                     /old_locus_tag="pgaptmp_001318"
     CDS             complement(3768..4793)
                     /gene="nagZ"
                     /locus_tag="ID725_RS06590"
                     /old_locus_tag="pgaptmp_001318"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGV7849956.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4804..5628)
                     /gene="thiK"
                     /locus_tag="ID725_RS06595"
                     /old_locus_tag="pgaptmp_001319"
     CDS             complement(4804..5628)
                     /gene="thiK"
                     /locus_tag="ID725_RS06595"
                     /old_locus_tag="pgaptmp_001319"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGV7849957.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLHQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5609..6250)
                     /gene="lpoB"
                     /locus_tag="ID725_RS06600"
                     /old_locus_tag="pgaptmp_001320"
     CDS             complement(5609..6250)
                     /gene="lpoB"
                     /locus_tag="ID725_RS06600"
                     /old_locus_tag="pgaptmp_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGV7849958.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6264..6641)
                     /gene="ycfL"
                     /locus_tag="ID725_RS06605"
                     /old_locus_tag="pgaptmp_001321"
     CDS             complement(6264..6641)
                     /gene="ycfL"
                     /locus_tag="ID725_RS06605"
                     /old_locus_tag="pgaptmp_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGV7849959.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6644..7003)
                     /gene="hinT"
                     /locus_tag="ID725_RS06610"
                     /old_locus_tag="pgaptmp_001322"
     CDS             complement(6644..7003)
                     /gene="hinT"
                     /locus_tag="ID725_RS06610"
                     /old_locus_tag="pgaptmp_001322"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGV7849960.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7134..7322
                     /locus_tag="ID725_RS06615"
                     /old_locus_tag="pgaptmp_001323"
     CDS             7134..7322
                     /locus_tag="ID725_RS06615"
                     /old_locus_tag="pgaptmp_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7849961.1"
                     /translation="MLPYISPLFPFLPANALKRHSLFGLHDKYECVYFSFAFAFAFAF
                     ASKIIRTLNITAHHPPPA"
     gene            7355..9544
                     /gene="fhuE"
                     /locus_tag="ID725_RS06620"
                     /old_locus_tag="pgaptmp_001324"
     CDS             7355..9544
                     /gene="fhuE"
                     /locus_tag="ID725_RS06620"
                     /old_locus_tag="pgaptmp_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGV7849962.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9604..11037)
                     /gene="ptsG"
                     /locus_tag="ID725_RS06625"
                     /old_locus_tag="pgaptmp_001325"
     CDS             complement(9604..11037)
                     /gene="ptsG"
                     /locus_tag="ID725_RS06625"
                     /old_locus_tag="pgaptmp_001325"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGV7849963.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11332..12129)
                     /gene="ycfH"
                     /locus_tag="ID725_RS06630"
                     /old_locus_tag="pgaptmp_001326"
     CDS             complement(11332..12129)
                     /gene="ycfH"
                     /locus_tag="ID725_RS06630"
                     /old_locus_tag="pgaptmp_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGV7849964.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12140..13144)
                     /gene="holB"
                     /locus_tag="ID725_RS06635"
                     /old_locus_tag="pgaptmp_001327"
     CDS             complement(12140..13144)
                     /gene="holB"
                     /locus_tag="ID725_RS06635"
                     /old_locus_tag="pgaptmp_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGV7849965.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13141..13782)
                     /gene="tmk"
                     /locus_tag="ID725_RS06640"
                     /old_locus_tag="pgaptmp_001328"
     CDS             complement(13141..13782)
                     /gene="tmk"
                     /locus_tag="ID725_RS06640"
                     /old_locus_tag="pgaptmp_001328"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGV7849966.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(13772..14794)
                     /gene="yceG"
                     /locus_tag="ID725_RS06645"
                     /old_locus_tag="pgaptmp_001329"
     CDS             complement(13772..14794)
                     /gene="yceG"
                     /locus_tag="ID725_RS06645"
                     /old_locus_tag="pgaptmp_001329"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGV7849967.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14797..15606)
                     /gene="pabC"
                     /locus_tag="ID725_RS06650"
                     /old_locus_tag="pgaptmp_001330"
     CDS             complement(14797..15606)
                     /gene="pabC"
                     /locus_tag="ID725_RS06650"
                     /old_locus_tag="pgaptmp_001330"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGV7849968.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15726..16967)
                     /gene="fabF"
                     /locus_tag="ID725_RS06655"
                     /old_locus_tag="pgaptmp_001331"
     CDS             complement(15726..16967)
                     /gene="fabF"
                     /locus_tag="ID725_RS06655"
                     /old_locus_tag="pgaptmp_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGV7849969.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17055..17291)
                     /gene="acpP"
                     /locus_tag="ID725_RS06660"
                     /old_locus_tag="pgaptmp_001332"
     CDS             complement(17055..17291)
                     /gene="acpP"
                     /locus_tag="ID725_RS06660"
                     /old_locus_tag="pgaptmp_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGV7849970.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17502..18236)
                     /gene="fabG"
                     /locus_tag="ID725_RS06665"
                     /old_locus_tag="pgaptmp_001333"
     CDS             complement(17502..18236)
                     /gene="fabG"
                     /locus_tag="ID725_RS06665"
                     /old_locus_tag="pgaptmp_001333"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGV7849971.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18249..19178)
                     /gene="fabD"
                     /locus_tag="ID725_RS06670"
                     /old_locus_tag="pgaptmp_001334"
     CDS             complement(18249..19178)
                     /gene="fabD"
                     /locus_tag="ID725_RS06670"
                     /old_locus_tag="pgaptmp_001334"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGV7849972.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19194..20147)
                     /gene="fabH"
                     /locus_tag="ID725_RS06675"
                     /old_locus_tag="pgaptmp_001335"
     CDS             complement(19194..20147)
                     /gene="fabH"
                     /locus_tag="ID725_RS06675"
                     /old_locus_tag="pgaptmp_001335"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGV7849973.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20215..21285)
                     /gene="plsX"
                     /locus_tag="ID725_RS06680"
                     /old_locus_tag="pgaptmp_001336"
     CDS             complement(20215..21285)
                     /gene="plsX"
                     /locus_tag="ID725_RS06680"
                     /old_locus_tag="pgaptmp_001336"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGV7849974.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21366..21539)
                     /gene="rpmF"
                     /locus_tag="ID725_RS06685"
                     /old_locus_tag="pgaptmp_001337"
     CDS             complement(21366..21539)
                     /gene="rpmF"
                     /locus_tag="ID725_RS06685"
                     /old_locus_tag="pgaptmp_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGV7849975.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21591..22112)
                     /gene="yceD"
                     /locus_tag="ID725_RS06690"
                     /old_locus_tag="pgaptmp_001338"
     CDS             complement(21591..22112)
                     /gene="yceD"
                     /locus_tag="ID725_RS06690"
                     /old_locus_tag="pgaptmp_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGV7849976.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22311..22895
                     /gene="yceF"
                     /locus_tag="ID725_RS06695"
                     /old_locus_tag="pgaptmp_001339"
     CDS             22311..22895
                     /gene="yceF"
                     /locus_tag="ID725_RS06695"
                     /old_locus_tag="pgaptmp_001339"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGV7849977.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22996..23955)
                     /gene="rluC"
                     /locus_tag="ID725_RS06700"
                     /old_locus_tag="pgaptmp_001340"
     CDS             complement(22996..23955)
                     /gene="rluC"
                     /locus_tag="ID725_RS06700"
                     /old_locus_tag="pgaptmp_001340"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGV7849978.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24528..27713
                     /gene="rne"
                     /locus_tag="ID725_RS06705"
                     /old_locus_tag="pgaptmp_001341"
     CDS             24528..27713
                     /gene="rne"
                     /locus_tag="ID725_RS06705"
                     /old_locus_tag="pgaptmp_001341"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGV7849979.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27908..28861)
                     /gene="flgL"
                     /locus_tag="ID725_RS06710"
                     /old_locus_tag="pgaptmp_001342"
     CDS             complement(27908..28861)
                     /gene="flgL"
                     /locus_tag="ID725_RS06710"
                     /old_locus_tag="pgaptmp_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGV7849980.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28873..30516)
                     /gene="flgK"
                     /locus_tag="ID725_RS06715"
                     /old_locus_tag="pgaptmp_001343"
     CDS             complement(28873..30516)
                     /gene="flgK"
                     /locus_tag="ID725_RS06715"
                     /old_locus_tag="pgaptmp_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGV7849981.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30582..31523)
                     /gene="flgJ"
                     /locus_tag="ID725_RS06720"
                     /old_locus_tag="pgaptmp_001344"
     CDS             complement(30582..31523)
                     /gene="flgJ"
                     /locus_tag="ID725_RS06720"
                     /old_locus_tag="pgaptmp_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGV7849982.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31523..32620)
                     /gene="flgI"
                     /locus_tag="ID725_RS06725"
                     /old_locus_tag="pgaptmp_001345"
     CDS             complement(31523..32620)
                     /gene="flgI"
                     /locus_tag="ID725_RS06725"
                     /old_locus_tag="pgaptmp_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGV7849983.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32632..33330)
                     /gene="flgH"
                     /locus_tag="ID725_RS06730"
                     /old_locus_tag="pgaptmp_001346"
     CDS             complement(32632..33330)
                     /gene="flgH"
                     /locus_tag="ID725_RS06730"
                     /old_locus_tag="pgaptmp_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGV7849984.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33383..34165)
                     /gene="flgG"
                     /locus_tag="ID725_RS06735"
                     /old_locus_tag="pgaptmp_001347"
     CDS             complement(33383..34165)
                     /gene="flgG"
                     /locus_tag="ID725_RS06735"
                     /old_locus_tag="pgaptmp_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGV7849985.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34303..35058)
                     /gene="flgF"
                     /locus_tag="ID725_RS06740"
                     /old_locus_tag="pgaptmp_001348"
     CDS             complement(34303..35058)
                     /gene="flgF"
                     /locus_tag="ID725_RS06740"
                     /old_locus_tag="pgaptmp_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGV7849986.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35078..36283)
                     /gene="flgE"
                     /locus_tag="ID725_RS06745"
                     /old_locus_tag="pgaptmp_001349"
     CDS             complement(35078..36283)
                     /gene="flgE"
                     /locus_tag="ID725_RS06745"
                     /old_locus_tag="pgaptmp_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGV7849987.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36308..37003)
                     /gene="flgD"
                     /locus_tag="ID725_RS06750"
                     /old_locus_tag="pgaptmp_001350"
     CDS             complement(36308..37003)
                     /gene="flgD"
                     /locus_tag="ID725_RS06750"
                     /old_locus_tag="pgaptmp_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGV7849988.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37015..37419)
                     /gene="flgC"
                     /locus_tag="ID725_RS06755"
                     /old_locus_tag="pgaptmp_001351"
     CDS             complement(37015..37419)
                     /gene="flgC"
                     /locus_tag="ID725_RS06755"
                     /old_locus_tag="pgaptmp_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGV7849989.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37423..37839)
                     /gene="flgB"
                     /locus_tag="ID725_RS06760"
                     /old_locus_tag="pgaptmp_001352"
     CDS             complement(37423..37839)
                     /gene="flgB"
                     /locus_tag="ID725_RS06760"
                     /old_locus_tag="pgaptmp_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGV7849990.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37994..38653
                     /gene="flgA"
                     /locus_tag="ID725_RS06765"
                     /old_locus_tag="pgaptmp_001353"
     CDS             37994..38653
                     /gene="flgA"
                     /locus_tag="ID725_RS06765"
                     /old_locus_tag="pgaptmp_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGV7849991.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38729..39022
                     /gene="flgM"
                     /locus_tag="ID725_RS06770"
                     /old_locus_tag="pgaptmp_001354"
     CDS             38729..39022
                     /gene="flgM"
                     /locus_tag="ID725_RS06770"
                     /old_locus_tag="pgaptmp_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGV7849992.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39027..39443
                     /gene="flgN"
                     /locus_tag="ID725_RS06775"
                     /old_locus_tag="pgaptmp_001355"
     CDS             39027..39443
                     /gene="flgN"
                     /locus_tag="ID725_RS06775"
                     /old_locus_tag="pgaptmp_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGV7849993.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39483..41018)
                     /gene="murJ"
                     /locus_tag="ID725_RS06780"
                     /old_locus_tag="pgaptmp_001356"
     CDS             complement(39483..41018)
                     /gene="murJ"
                     /locus_tag="ID725_RS06780"
                     /old_locus_tag="pgaptmp_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGV7849994.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41128..42051)
                     /gene="yceM"
                     /locus_tag="ID725_RS06785"
                     /old_locus_tag="pgaptmp_001357"
     CDS             complement(41128..42051)
                     /gene="yceM"
                     /locus_tag="ID725_RS06785"
                     /old_locus_tag="pgaptmp_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGV7849995.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42053..42700)
                     /gene="yceH"
                     /locus_tag="ID725_RS06790"
                     /old_locus_tag="pgaptmp_001358"
     CDS             complement(42053..42700)
                     /gene="yceH"
                     /locus_tag="ID725_RS06790"
                     /old_locus_tag="pgaptmp_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGV7849996.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42711..43295)
                     /gene="rimJ"
                     /locus_tag="ID725_RS06795"
                     /old_locus_tag="pgaptmp_001359"
     CDS             complement(42711..43295)
                     /gene="rimJ"
                     /locus_tag="ID725_RS06795"
                     /old_locus_tag="pgaptmp_001359"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGV7849997.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43531..44739
                     /gene="mdtH"
                     /locus_tag="ID725_RS06800"
                     /old_locus_tag="pgaptmp_001360"
     CDS             43531..44739
                     /gene="mdtH"
                     /locus_tag="ID725_RS06800"
                     /old_locus_tag="pgaptmp_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGV7849998.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44803..45450
                     /gene="grxB"
                     /locus_tag="ID725_RS06805"
                     /old_locus_tag="pgaptmp_001361"
     CDS             44803..45450
                     /gene="grxB"
                     /locus_tag="ID725_RS06805"
                     /old_locus_tag="pgaptmp_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGV7849999.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45584..46144
                     /gene="yceB"
                     /locus_tag="ID725_RS06810"
                     /old_locus_tag="pgaptmp_001362"
     CDS             45584..46144
                     /gene="yceB"
                     /locus_tag="ID725_RS06810"
                     /old_locus_tag="pgaptmp_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGV7850000.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46250..47296
                     /gene="pyrC"
                     /locus_tag="ID725_RS06815"
                     /old_locus_tag="pgaptmp_001363"
     CDS             46250..47296
                     /gene="pyrC"
                     /locus_tag="ID725_RS06815"
                     /old_locus_tag="pgaptmp_001363"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGV7850001.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47370..47615
                     /gene="dinI"
                     /locus_tag="ID725_RS06820"
                     /old_locus_tag="pgaptmp_001364"
     CDS             47370..47615
                     /gene="dinI"
                     /locus_tag="ID725_RS06820"
                     /old_locus_tag="pgaptmp_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGV7850002.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47905..48159
                     /gene="bssS"
                     /locus_tag="ID725_RS06825"
                     /old_locus_tag="pgaptmp_001365"
     CDS             47905..48159
                     /gene="bssS"
                     /locus_tag="ID725_RS06825"
                     /old_locus_tag="pgaptmp_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGV7850003.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48274..49392
                     /gene="solA"
                     /locus_tag="ID725_RS06830"
                     /old_locus_tag="pgaptmp_001366"
     CDS             48274..49392
                     /gene="solA"
                     /locus_tag="ID725_RS06830"
                     /old_locus_tag="pgaptmp_001366"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGV7850004.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            49440..49553
                     /locus_tag="ID725_RS06835"
                     /old_locus_tag="pgaptmp_001367"
     CDS             49440..49553
                     /locus_tag="ID725_RS06835"
                     /old_locus_tag="pgaptmp_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGV7850005.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49814..50380
                     /gene="yceJ"
                     /locus_tag="ID725_RS06840"
                     /old_locus_tag="pgaptmp_001368"
     CDS             49814..50380
                     /gene="yceJ"
                     /locus_tag="ID725_RS06840"
                     /old_locus_tag="pgaptmp_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGV7850006.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50384..50959
                     /gene="yceI"
                     /locus_tag="ID725_RS06845"
                     /old_locus_tag="pgaptmp_001369"
     CDS             50384..50959
                     /gene="yceI"
                     /locus_tag="ID725_RS06845"
                     /old_locus_tag="pgaptmp_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGV7850007.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51001..52053)
                     /gene="trhO"
                     /locus_tag="ID725_RS06850"
                     /old_locus_tag="pgaptmp_001370"
     CDS             complement(51001..52053)
                     /gene="trhO"
                     /locus_tag="ID725_RS06850"
                     /old_locus_tag="pgaptmp_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGV7850008.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52278..53198
                     /gene="lpxL"
                     /locus_tag="ID725_RS06855"
                     /old_locus_tag="pgaptmp_001371"
     CDS             52278..53198
                     /gene="lpxL"
                     /locus_tag="ID725_RS06855"
                     /old_locus_tag="pgaptmp_001371"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGV7850009.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53370..54596
                     /gene="mdtG"
                     /locus_tag="ID725_RS06860"
                     /old_locus_tag="pgaptmp_001372"
                     /pseudo
     CDS             53370..54596
                     /gene="mdtG"
                     /locus_tag="ID725_RS06860"
                     /old_locus_tag="pgaptmp_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            54679..55053
                     /gene="msyB"
                     /locus_tag="ID725_RS06865"
                     /old_locus_tag="pgaptmp_001373"
     CDS             54679..55053
                     /gene="msyB"
                     /locus_tag="ID725_RS06865"
                     /old_locus_tag="pgaptmp_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGV7850010.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(55054..55281)
                     /gene="yceK"
                     /locus_tag="ID725_RS06870"
                     /old_locus_tag="pgaptmp_001374"
     CDS             complement(55054..55281)
                     /gene="yceK"
                     /locus_tag="ID725_RS06870"
                     /old_locus_tag="pgaptmp_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGV7850011.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(55454..57997)
                     /gene="mdoH"
                     /locus_tag="ID725_RS06875"
                     /old_locus_tag="pgaptmp_001375"
     CDS             complement(55454..57997)
                     /gene="mdoH"
                     /locus_tag="ID725_RS06875"
                     /old_locus_tag="pgaptmp_001375"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGV7850012.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(57990..59525)
                     /gene="mdoG"
                     /locus_tag="ID725_RS06880"
                     /old_locus_tag="pgaptmp_001376"
     CDS             complement(57990..59525)
                     /gene="mdoG"
                     /locus_tag="ID725_RS06880"
                     /old_locus_tag="pgaptmp_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGV7850013.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            59918..61075
                     /gene="mdoC"
                     /locus_tag="ID725_RS06885"
                     /old_locus_tag="pgaptmp_001377"
     CDS             59918..61075
                     /gene="mdoC"
                     /locus_tag="ID725_RS06885"
                     /old_locus_tag="pgaptmp_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGV7850014.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(61083..62564)
                     /gene="clsC"
                     /locus_tag="ID725_RS06890"
                     /old_locus_tag="pgaptmp_001378"
     CDS             complement(61083..62564)
                     /gene="clsC"
                     /locus_tag="ID725_RS06890"
                     /old_locus_tag="pgaptmp_001378"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGV7850015.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(62506..63039)
                     /gene="ymdB"
                     /locus_tag="ID725_RS06895"
                     /old_locus_tag="pgaptmp_001379"
     CDS             complement(62506..63039)
                     /gene="ymdB"
                     /locus_tag="ID725_RS06895"
                     /old_locus_tag="pgaptmp_001379"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGV7850016.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(63134..63445)
                     /gene="ymdA"
                     /locus_tag="ID725_RS06900"
                     /old_locus_tag="pgaptmp_001380"
     CDS             complement(63134..63445)
                     /gene="ymdA"
                     /locus_tag="ID725_RS06900"
                     /old_locus_tag="pgaptmp_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGV7850017.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(63566..63898)
                     /gene="csgC"
                     /locus_tag="ID725_RS06905"
                     /old_locus_tag="pgaptmp_001381"
     CDS             complement(63566..63898)
                     /gene="csgC"
                     /locus_tag="ID725_RS06905"
                     /old_locus_tag="pgaptmp_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGV7850018.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(63957..64415)
                     /gene="csgA"
                     /locus_tag="ID725_RS06910"
                     /old_locus_tag="pgaptmp_001382"
     CDS             complement(63957..64415)
                     /gene="csgA"
                     /locus_tag="ID725_RS06910"
                     /old_locus_tag="pgaptmp_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGV7850019.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(64456..64911)
                     /gene="csgB"
                     /locus_tag="ID725_RS06915"
                     /old_locus_tag="pgaptmp_001383"
     CDS             complement(64456..64911)
                     /gene="csgB"
                     /locus_tag="ID725_RS06915"
                     /old_locus_tag="pgaptmp_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGV7850020.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        15855 a        17282 c        15982 g        15900 t
ORIGIN      
        1 ataacctttc attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat
       61 gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg
      121 ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac ctcgtcgaag
      181 cgaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag gggttatttc
      241 gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt
      301 gtgaagagaa aagcgtggag tctgcgctgg ctgactattt tgctgccatc gccaactgct
      361 ttaccggcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc accctctccc
      421 cagattcagg agatatcgcc aatacgttga aatcacgcca tgcgatgcaa gagcgcactt
      481 tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga
      541 cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag
      601 gtgcatcgtt ggaaaaactg atgcagattg ccggaacgac tttgcgttta tggcccgaac
      661 tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg aatgaggggc caaattgcag
      721 agtacagcct tgttatactt tgttattcaa aatcagttgc ccgttactat ctagcgggat
      781 ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt
      841 cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt
      901 agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg catatccacc
      961 acccagcgcc cccacaacag tggcgacatc tttaccacga ccaccaccaa actgatgccc
     1021 tatcacgccg ccagcaacag cgccgagcac cgacccggta atgcgatgtt catcctgcac
     1081 cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc
     1141 cttgattggg gttgcagaaa caacctgagc gtattgcggg ccacgttcaa acacgttcag
     1201 actggccact gccgctacgc caagcgcagc tgcgacacca atcccgatac ccgccaacat
     1261 tgatttattc acgggaatac ctccttcttt gcagttatgc caatcatgca agcgaaggag
     1321 aaaagcagct atcagacaga agatgaaaag cgcgggaatg gctggaaata cttaaggatt
     1381 cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct
     1441 cgtaccattt gtccagggcg gatgaggcgt ttatgccaca tccgccagtg tacgtcgatt
     1501 aatgcaactt caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc
     1561 cggttttaaa gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa
     1621 agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac
     1681 caacggtgga gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt
     1741 tcagcagctt accgttcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt
     1801 gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt
     1861 cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag cgtcagttcc accaccagct
     1921 ggttgatacg gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc
     1981 cggctgccca taccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc
     2041 cttcatcagc actggtgacc atggtttgcg tcagaacgcg aacgccaagt ttcgttagct
     2101 cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt
     2161 ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt
     2221 gcttgaccgc gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa
     2281 tcgcaatgtt cactttgcca tttgcgccca ggttggcgga gtatttcagg aagagattca
     2341 gcatctcctg gtgaaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt
     2401 tgacacctgg tgtattgaaa tcgttagagg tgctacccag cgccattacc agggtgtcat
     2461 aggcgatttt acgttccgga accagcagtt cacctttctc atcgcgcagt tctgcaatag
     2521 tgattgtttt cgcgtcacga tcaatatcaa tgacggaacc cagctggaac tggaaaccat
     2581 gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg
     2641 ctacttcgtg cagcagcggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt
     2701 tggctttttt cttgcgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag
     2761 caccgccgcc gacaatcaca atttttttca atggcgtagt caacgtgacc cccttaaatt
     2821 tattaaccaa ttgttaatta atggttatta acatagctct taattaacaa caggttacag
     2881 caatgaaaat gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat
     2941 tgatgttaat caagttttgt tgctgaaaag ataggcgact ttaccgggtg agatcaaaaa
     3001 acagagttta ttgtttatta tttgagcgaa tttgtgagag tgatatagcg tgaggggtgt
     3061 atgtagggcg ttcggtaggt ctgataagag gcgcatcagg cattgatgct cgactgtcgg
     3121 atgcggcgtg aacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg
     3181 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgcaaat
     3241 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
     3301 gatgcaactc ttcagatgtc cgctggctgt taagcgcttc atcattacgc gacaaaatca
     3361 ccaggcaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg
     3421 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
     3481 tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
     3541 gaccaacgcc gcaaatcagg ggacgctcgt cgacgttcag ttgcaacatt ttgtccactt
     3601 ctttgagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
     3661 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
     3721 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctgct
     3781 ttctcttcct gccagcgttc atgtaactga ttcagacggg cgctgatcgc tttccagcga
     3841 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
     3901 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
     3961 accaggatca tatcgcaacc cgcatccagt gatacctgcc cgcgttcggc ataactgccc
     4021 ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaacccagt
     4081 tcctgacgca aaacggtttt cagccagtag gaagaaccgc tcgccggacg cggatcaaca
     4141 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaacgtg
     4201 ctgaaaaccg acatatcttt ggcacgaatt tccgcttgcg ggcgtgggtc gcacggcgtc
     4261 tctttgtgtg aatctgcagt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
     4321 attccggctt catgcatccc atcaataaac cgactggcga ttgccagggc tttttctggg
     4381 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gtcctacatc cagcactggc
     4441 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
     4501 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
     4561 aagcgggtaa aaccttcgcg aaaacgctgg acgcgtccac cttcctgatc taccgccacc
     4621 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgcaa ctgggcagga
     4681 tcatgatagt ttcgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
     4741 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
     4801 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
     4861 atcgccggtt tgtcgccagc ggtactcaaa ccaccctgct ttgagcatca gcagccaggg
     4921 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
     4981 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
     5041 cgcgatatca ccatctcctg catactccca gtcgatgagt tttaaccctg acgcgctatg
     5101 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gccgtggttc
     5161 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc actgtccgcc gcgccggatc
     5221 gctttgctgc cagtacagtt ccagtaacgg caacagcgtt attcgccagc caaaacgtgg
     5281 ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
     5341 tacctcgccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
     5401 tgcaatgcat gcgggtagtt gtgataaagc ccgatactgg tgcaagaacg cggactgcgg
     5461 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
     5521 actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
     5581 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
     5641 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
     5701 tgccagaggc gctggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg
     5761 ctttactacg ggtgcctaaa ctatcctgcg gcgacaaacc taactgttgc ttagccatcg
     5821 acagctgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg
     5881 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
     5941 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
     6001 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgcagtt tgatcttcgt
     6061 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
     6121 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
     6181 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
     6241 tttttgtcat caagattcac cccttacaaa tatagataaa gtctgacttt gtgcgccccc
     6301 agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatggt aacgctgcgt
     6361 ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa acgataatga
     6421 acggtaacgg gttcttgcct ttcgttataa agcgttgagg atgctgaagg ttgaatatca
     6481 gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga tgactccatc
     6541 accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc caccagcagt
     6601 aacaacgcca gagacaccag cccaaagcat ccttttctca tcgttacaga cctttatgcg
     6661 ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg taaacctctt
     6721 gtccgccatg gcggttggtg ttcatgatca gacgatagcc atcttcggca ataccttctt
     6781 gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca tgctcagctg
     6841 agacgtcgtt cacagtcgga atgaggatat tcggaatgat cagaatatgc gttggcgctt
     6901 gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata tcggagggga
     6961 tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt tcctttttca
     7021 aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataga gtatggtcga
     7081 gttaaacctg ctctttcaac cttaactcac gatttcttaa gcaaaaaatc gttttgctgc
     7141 cgtatatatc tccattattc ccatttctgc ctgcgaatgc gctgaaaaga cattctctgt
     7201 ttggtttaca cgacaaatat gaatgcgtat atttctcatt tgcatttgca tttgcatttg
     7261 catttgcaag caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat
     7321 gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga
     7381 taatcaatat caagccatca ccaaaccgtc actacttgcc ggttgcatag cactggcact
     7441 attaccttct gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc
     7501 agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac
     7561 caaaatgcag atgactcaac gtgatattcc tcagtcggtc actattgtta gccagcagcg
     7621 gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag
     7681 caaaagtcag gcggattccg atcgtgctct ttattattcc cgcggattcc agatcgataa
     7741 ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact
     7801 ttctgatatg gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac
     7861 cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga ccagtcgtga
     7921 atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga
     7981 tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca
     8041 gaataacgac tcatggctgg accgctacaa tagtgaaaag accttcttct ccggcattgt
     8101 cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga
     8161 tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag
     8221 ttacgatcgc gcacgcagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa
     8281 ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac
     8341 ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc
     8401 ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatt atgtcggcgg
     8461 caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag
     8521 ttatgaattg tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa
     8581 caatcgttac ttcagttcat gggccaacat cttcccggat gaaattggca gtttctacaa
     8641 ctttaatggc aatttcccac aaaccgactg gtcaccacag agcctggcgc aggacgatac
     8701 cacacatatg aaatcgttat atgctgccac tcgtgtcacc cttgccgatc cgctgcatct
     8761 gatcctcggc gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa
     8821 aaaccacacc acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta
     8881 cgccagctat acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct
     8941 ggctccaatc accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg
     9001 tctgaccacc acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg
     9061 tacacctatc cccggcagca acggcgaaac cgcctataaa gcggtggatg ggacagtcag
     9121 taaaggggtg gaatttgaac tcaacggcgc aattaccgac aactggcagc tgacatttgg
     9181 cgcaacgcgc tatattgcag aggataacga aggaaacgcc gttaatccta atctgccacg
     9241 caccacggtt aaaatgttca ccagctatcg gttgcctgtc atgccagagt tgacagtcgg
     9301 cggtggtgtt aactggcaaa atcgcgtgta taccgacacc gtgacaccgt atggcacctt
     9361 ccgcgccgag caaggtagct acgcgttggt ggatctcttc acccgctacc aggtgacgaa
     9421 aaacttctcg ttacagggga acgtcaataa cctgttcgac aaaacctacg ataccaacgt
     9481 ggaaggttct atcgtctacg gcgcaccgcg taatttcagc attaccggca cgtatcaatt
     9541 ctgaataaca cctgtaaaaa aggcagccat ctggctgcct tagtctcccc aacgtcttac
     9601 ggattagtgg ttacggatgt actcatccat ctcggttttc aggttatcgg atttagtacc
     9661 gaaaatcgcc tgaacaccag aaccagcaac cactacgccc gctgcgccca gtttcttcag
     9721 gccagcctga tccactttag acacatcagc aacgctgacg cgcagacggg taatacatgc
     9781 gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca accagagccg gtgccatttc
     9841 gctggtacct gtcgcttttg catcttcagt cgcgtcttca cgacccggcg ttttcagatc
     9901 cagtgcttta atcagcacgc ggaagatggt gtagtaaaca atcgcataac cgataccgac
     9961 gatcgggaac agccacagtt tgctgctgtt accagacaga acgatgaagt cgatcagacc
    10021 gtgcgaaaac gacgtaccgt cacgcatccc cagaagaata cagattggga atgccaggcc
    10081 tgccagaatc gcgtggatga tgtacaggat cggcgcaacg aacatgaagg agaactcgat
    10141 cggctcggtg ataccggtca ggaacgaggt cagcgccgcg gagatcataa taccgcccac
    10201 tttcgcgcgg ttttctggtt tagcagagtg ccagatagca attgcggcag ctggcagacc
    10261 gtacattttg aacaggaagc caccagacag tttacccgca gtcgggtcac ccgccatata
    10321 acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac caatctgcat
    10381 ctggaaaggt acgttccaga tgtggtgcag accaaacggt accaggcaac gttcgatgaa
    10441 accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag agaaggtctg
    10501 gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa agatggcagc
    10561 caggccagaa atgatcggca caaagcgttt accggcaaag aagccaagat actcaggcag
    10621 cttaatacgg tagaaacggt taaacatgta cgctgcgatc gcaccggaga taatccctcc
    10681 gagtacgcca gtatccgcca ggtgtttaga ggcgatttct tcagcaggta aatgcagtac
    10741 cagtggcgca accacggcca tggttttaac catgatgcca taggcaacaa ctgcagccag
    10801 cgcggatacg ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg
    10861 catgtttgca aagacggaac cgcctgcttc tgccataaca tgcgatacaa cggcgggcag
    10921 ccagctgaaa ttcgcggaac cgacgcccag cagaatacct gcgataggca gtacggatac
    10981 cggcagcatc agcgatttac cgaccttttg caggttagca aatgcattct taaacataat
    11041 tgagagtgct cctgagtatg ggtgcttttt ttacgttctc acgcgtggca aggggggaga
    11101 gcctcgccgt gtacagggca tctaagcgcc ctttatttat tacacagagt aaaataattc
    11161 agtgccaatt tgtttgacgg ctatcacgtt tcaacttcag atcattggag aagttgcaca
    11221 ttttgacaaa acatatgtca caatatttca aacgtggtta ttcaccgccc aattttcggc
    11281 ggtgaacttt actcgtttta gtcattaatt acgagcttta aaaaaactca ttcaacggat
    11341 ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg tggttacctg
    11401 cgccagttct tcaacggcaa cacctttcaa cacagccatg tattctgcaa cgtcacgaac
    11461 catcgcaggt tgattctctt ttcctcgatg cggcaccggc gcaaggtaag gtgagtcagt
    11521 ttccaccagt aaccgatcca gggggacata acgcgcagca tcgcgcagtt gctccgcatt
    11581 acggaaggtc acaatgccgg aaaaggagat gtaaaatccg agatccagta atttgcccgc
    11641 cgtttctctg tcctctgtaa aacagtgtag tacgccaccg caatccgtca ctttttcttc
    11701 gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta tggacgataa ccggcttgtt
    11761 cagttcacga ccaatctgga tatgatggat gaaggactct tgctgacgta ctttagtttc
    11821 cggtgtgtaa taataatcca gcccggtttc acccagcgct acaacaccct cttctgccgc
    11881 cagacggcgt aaatcttcta catcgtaggg atcattctgg ttgagtggat gcacgccaca
    11941 agaaaatacg acgttgtcac gttcgcctac cagatcccgc atgtgtaaat aacccggtaa
    12001 tgttgtggcg actgccagac aaaatttcac atcgcgtgcg gcggctttcg ccagaacgtc
    12061 atccacgtcc ttatgcaaag attcataatc cagaccatcg agatggcagt gtgagtcgac
    12121 taaaaacatg atgtctctct taaagatgag gaaccggtag cacaacgccc ggttgcaggt
    12181 aatgctcaat acgcagtaaa agatcggtga tgagaagctc gcggttgatg cctgtgacag
    12241 acattaactg ttcacgaatg tggcaaacat cccccagtat agcctgcagg cgcgagggag
    12301 aaagatggtt tgccagttcg gcgaccaggc ccggcacatc aacattggtc acctgcgcag
    12361 cgccatgatg gcgttttagc gcatccatca gcaacgttgc cagccagtgt aaacgcgccg
    12421 gagcttgttc atgattaagg gccgccagca gcgaatacca gtcgcccgat ggcacgctat
    12481 atgccaacgc ctgacacaat gtttcacgag cctgccagtt atctccctga aacaacgcca
    12541 gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca tcctgtgaca
    12601 ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc cggcggcggc gcaaggtaat
    12661 gtaaccgaca acgactacgt aacgttgcca gtaaacgttc aggctcgcgg gtagccagga
    12721 aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt
    12781 cggtcagtaa ggcagcatcg gttacccaga caactttcgc accacctaag cgtgcgtgct
    12841 cattcagctt ttcggtgacc tcacgtaccg catcaatgcc cagcgtattt ttcccttttt
    12901 cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg catcaactga catccacgac
    12961 agtgaccgca acttttgtgg ccctgcggtt gttggcagag taaataacgg ctcagggcgt
    13021 agattaaagc atcatcgccc atgcccggta acgcctgaat gagtagcgca tggtgacctc
    13081 ttccggcctg atagctggct accagttttt cgaaatcagg tcgtaaccat ggataccatc
    13141 tcatgcgtcc aactccttca cccagcgggt cacggtagtg cggattgcat ccatcacggc
    13201 ctccagcggc tgggtggcat caatggtatg aatactttta tcttgtgcag ccagttccag
    13261 atagcgggcg cgggtgcgat taaagaaatc gaaagactct tgctcaatac gatccagctc
    13321 gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta acatcgagat agagcgttaa
    13381 gtcggggcga aaatccccga gaacagcatc acgcagtgtt gccagcatat gttggtcaat
    13441 accacgtcct ccgccctgat acgcctgagt ggagagatcg tggcgatcgc caataaccca
    13501 ggtgccgtta gccagcgcag gtttgatgac cgtttctacc agttgaacgc gcgcggcata
    13561 aaacatcaga acttcggctt tatcggtaat gatttcatcg cctaccgatt tgatatccag
    13621 caccaggctt cttaactttt cggcaagttg cgtaccacca ggttcccgag tgaaaaccat
    13681 gtcgcggata cccagttgct cgagcgtctc aaccaccaca ttacgcgcgg tagttttgcc
    13741 tgcgccttcc agcccctcaa tgacgatata cttactgcgc atttttttcc ttaagcactt
    13801 tcagataatc ctgcacagac ttgttatgac tggcaagatt ggtattaaac gtgtgaccac
    13861 ctttaccatc ggccacaaaa tagagatacg gcgtttttgc cggatgcgca gcagccttca
    13921 gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa accggtaatg gtataggtgt
    13981 tatacgctgt cggcgtttcc aggtctgcac gagaaagttt gccattataa cgctctccca
    14041 tcccgtaaat cacggttggg tcggtctgca ggcgcatacc aatgcgtaaa cggttgataa
    14101 ataccgaggc aacctgatcg cgttcactgg caatggcggt ttctttttcg ataattgatg
    14161 ccatcgtcac cagctgattt ttatctttat aaggcagacc gtccgcacgc ccttcccagg
    14221 cgctatcgac cgctttcacc attttcttgt gcgctcgctt gagtaacgcg acatcggtgg
    14281 tattggcggt atacatccag gtgtctggcc agaaccaacc ttcaatccac tccgggtttt
    14341 ccagttcaag tgcctgcgct acggtgatgt acttatcatc gctcagcgtg tgcttgatat
    14401 acggggcctc acgcaattgc ttgaggtagt cgctcagacg catcccttct accagtcgca
    14461 gagggaactg tgcttcttta ccgctttcca acaatttcag catctcgcgc acggtcatct
    14521 gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag atccggttcg atacgcagca
    14581 accattgaaa aacccgtggg cgattgatga tcttatcggc ataaagctgt tcaccaagcg
    14641 ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc ttctttgata agcaatttgc
    14701 tgtcggcaag atagcgaacc ttccagacgc ccacaccagc ggcgataccc agtaccacca
    14761 gcaataacaa gattatcaat aacacttttt tcatgactaa ttcgggcgct cacaaagtgg
    14821 ggctaaatat tcatataacg ttgctgacga aaaggagaca tcgccacagg cacgtacggg
    14881 catcactggc attaacgcat tacaaataac catctcatct gcctgcaacg cctcttccag
    14941 agaggcttgc acttcgacaa gctgataaga ggattgtgcc agcaaacgga tacagaattg
    15001 tcgcataatg ccgttaacac ctgcctgatc cagtcgcggc gtataaacta cgttgccctt
    15061 ccgccagaac aaattagctg cacagcattc cgtaaccccc ccttcgctgt caaggaccag
    15121 cgcctcatca gcgtttgtct gctcaagatg agagcggatc aatacttgct caagccgatt
    15181 aagatgttta atacctgcaa gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa
    15241 cgtgatccct tcgttacgca aacggtcgta atgtgcagga taagccgtaa cggagagaat
    15301 ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc ccgccactac cgcgactgat
    15361 cacgactttc agtacaccat tttgctgttc tgctgccagc gttttcatct cttgttcaag
    15421 ctgaggccag aagtcacagg aaatcatcaa ccgctgacaa gcatcctgta gtcgctggat
    15481 atgcgccgat aacaaactga ctttaccgtc gataactctg gcggtggtaa aacaaccatc
    15541 accaaactgc gttgcccgat cgcttactga cagcgattcc tgcttataac cgttaattaa
    15601 gaacataccg gctccttatc agagaattgt tagtgtggca gcatgttcac tacggaacaa
    15661 gtcggaataa aagctaagaa aaaaggcccg caagcggacc ttttatatgg gtggaaaatg
    15721 acaacttaga tctttttaaa gatcaaagaa ccattagtgc caccgaagcc gaaggagtta
    15781 cacagcgtgt attccattcc gctgacctga cgtgcttcat gcggtacgaa atccagatcg
    15841 caaccttcat ccgggttatc caggttgatg gttggcggaa cagcctgatc gcgcagcgcc
    15901 aggatggagt agatagattc tactgcaccc gccgcaccta acaggtgacc ggtcatagat
    15961 ttcgtggagc ttaccaacac acggcttgca gcttcaccga agatggtttt caccgcctgc
    16021 gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta gccaatctga
    16081 ctcgcttcaa tgcctgcatc acgcagagca tttgccattg ccagagctgc gcctgcgcca
    16141 ttttctggcg gtgacgtcat atgataagca tcgctgctca taccaaagcc gacgagttca
    16201 gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac cagcataccg
    16261 gcaccatcgc ccagtacgaa accatcacgc tctttatccc acgggcggct cgccgcttgc
    16321 gggttatcat tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac acccagcggc
    16381 gtactggctt tctctgcgcc acctgcaacc atcacgtcag catcgccata cgcgataata
    16441 cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg cggtcgcgat agagatgctt
    16501 gggccacgca ggccatacat gatagtcaga tgacctgcca ccatgttcac aatcgttgac
    16561 ggaacgaaga atgggctgat cttacgtgga ccaccgttca tcagagatgt gtggttttct
    16621 tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg caccaatgcg ggttgcgttc
    16681 tcttccgtta tttcaaggcc agaatcctgc atggcctgaa cgccagcgac aattccatat
    16741 tgaatgaagg catccatctt gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa
    16801 tcctttacta agccagcaaa tttcgttgca taggcgctag tatcgaaatg gtcgattagg
    16861 ctgatgccac tctgaccggc aagcagagct ttccaggtag actctacggt attgccgaca
    16921 ggagacaaca tgcccagtcc ggtcacaact acacgacgct tagacacgtt tgtcctccag
    16981 ggagggaaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc
    17041 ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc ctgaacggtg
    17101 gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc cagagccatt
    17161 accagctcaa cggtgtcaag agaatccgcg cctaggtctt caacgaaaga agcattgttg
    17221 gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac gcgttcttcg
    17281 atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt tttcgtagtg
    17341 tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga ttttacgtta
    17401 ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta ctcataacca
    17461 cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat gtacatcccg
    17521 ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc caggaatgca
    17581 accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg caccaggata
    17641 cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa gcccggagca
    17701 acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt actgaagccg
    17761 atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat ggtaccaacc
    17821 acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg cattaccgct
    17881 tttgacagac ggaaaacaga tgaaaggttg gtttcgataa tatcgttcca ctcttcatct
    17941 ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag gatatccact
    18001 tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg gtcggtcaca
    18061 ttcaacatca gacctttgcc gttagcacct aaataatcac tgatcgcctg agcgccattt
    18121 tcactggtcg cagtgccaat aactttcgcg ccacgggctg cgagcgtttc agcaattgcg
    18181 cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa attcatgatg
    18241 ttcctctttt aaagctcgag cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg
    18301 gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ctttgcccgg gccgacttca
    18361 tagagatgtt ctacgccttg cgctgccatg tactcaacag acttcgtcca ctgaaccggg
    18421 ttatacaact gacgtaccag tgcgtcacga atggcatcac cattggtttc gcatttcaca
    18481 tcaacgttat tcacaacagg aactgttggt gcgttaaagg tgattttcgc taattctact
    18541 gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct cactggtaac
    18601 ggcagagcgc gttttgcgcc cgcagcttta caggcagcgc cagcacgctc aaccgcttct
    18661 ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga aacgacctga
    18721 ccttctgcag cttcttcaca cgcttttcca atagacgcat catccagacc gatgattgcc
    18781 gccatagcgc ccgttccttc cggtacggct tcttgcatga acttgccgcg catctcaacc
    18841 agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa ccagcgcgga gtattccccc
    18901 aggctgtgac cggccatcat tgccggtgct ttaccgccat gctgctgcca tacgcggtac
    18961 agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc aggttttatt cagttcttca
    19021 gctggcccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga agcttcagca
    19081 aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc aacggtttga
    19141 gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa
    19201 cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg cttcaagcag aaccaactgc
    19261 cccggcttga tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac agaggccgca
    19321 gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatagacat accgagtttt
    19381 ttcgccgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca gtccagttga
    19441 gaacggtcaa gattattcgc cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc
    19501 gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc tggattcacg
    19561 cggtcggcgt ttggcagcgt cagcaattca ccataactac cgtcggcatg cagatgggtg
    19621 gaaatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa
    19681 ataataatag tcccacgatc tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg
    19741 accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct taatgcatag
    19801 gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc aacctttaat gcccaacatg
    19861 ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg
    19921 atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca
    19981 aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta
    20041 cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc gtttgtccgc
    20101 acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta cgctcagtca
    20161 cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg
    20221 cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata cagattccag
    20281 gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc
    20341 gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc
    20401 taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct
    20461 cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc cttcaccctg
    20521 agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta atgtgacatt
    20581 tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact cattggcttc
    20641 aagatagccg atataattga tagaagggat tgttttaagc accgctgagg catcccgaat
    20701 actatcgaga ccctttactt cttcttcacc aatattgagc aacgccacgc gaggattggg
    20761 aatttccacc acctcttcag ccaggactga gcccataatg gcaaattgca ccaacattgt
    20821 gctgtcacaa tcgacgttgg cccctaagtc aaggaccacc gttttgccct tttgctgatg
    20881 tggtaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga gtaataattt
    20941 tgccagcccc atcagtgccc cggtattacc ggcactgaca caggcttgcg ctcgaccttc
    21001 tttcaccagc tccagggcca cgcgcattga actcccacga ctggcgcgga tagcttgcga
    21061 aggccgggca tcactggcga taactgactg cgcaggaata atctgcagac gcgaacgttg
    21121 ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag
    21181 agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca cggaagggcc
    21241 aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc
    21301 cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat cacgcagacg
    21361 cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt gatgtggtga
    21421 cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc ggtcagcgcg
    21481 tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg tacggccatg
    21541 gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa ttacttacgc
    21601 tttaagctgg ctaatacggc aaatgggttt ggcttttgcg cttcttcagg cagttcacca
    21661 aagaccatgt ccgcttcgga cacttcacag tgttcagaat catgcaccgg aactaccggc
    21721 aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc gaattcgtta
    21781 acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga acgcacagga
    21841 ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg ctgacactcg
    21901 agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt atcgatagcg
    21961 aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc gacgcgctca
    22021 acctgatcag gagtatagat accctggtaa tcaaggcgtt tttgagccgt acgaaccgga
    22081 tcgagagtca ggggtaattt taccttttgc atagggcgcg catattaact ttgtaacgtc
    22141 atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg cacacattgc
    22201 gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg
    22261 catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat atgcctaaac
    22321 ttattttagc ctccacctcg ccctggcgtc gcgctcttct ggaaaaactg caaatctctt
    22381 ttgaatgtgc agcacccgag gtcgacgaaa ctccacacag cgacgaatca ccgcgacagt
    22441 tggtgcttcg actggcacaa gaaaaagcgc aatctctggc gtcacgttat ccggatcatt
    22501 taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa
    22561 cggaagaaaa tgcccgtctg caattacgca aagccagcgg caatatcgtc actttctata
    22621 ctggactggc gttgtttaat tcggcgaatg ggcatctgca aacagaagtg gagccttttg
    22681 acgtccattt ccgtcacctg agcgaggcgg agattgataa ttacgtgcgt aaagagcatc
    22741 ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattactctg tttgagcgtt
    22801 tagaggggcg tgatcctaac acgctggttg gtttaccgct aatcgcgctg tgtcagatgt
    22861 tacgtcggga agggaaaaac ccgctgatgg gatagttttt tgcgccggat gcggttacgt
    22921 cgcatccggc aatcggtgct ggcggcctga tgcggcgtaa acgccttatc ccattatcat
    22981 gcttatatgc tcgtattagc gcgcgttacg cagcttttgc aaacaacgct tcaaaccatc
    23041 atccatcggc gcttcgatac gcatcacctc accggtcccc ggatgggtaa acttcaacgc
    23101 tgcggcgtgc aggaacagac gatttaatcc cgtgcctgct tcagtgagct gtctgtcaaa
    23161 ttcacggtca ccgtagcgat cgtcaaaggc aatcggatga cctgcatact gtgtatgcac
    23221 acggatctga tgagtgcgcc ctgttaccgg actacaacgc accagggtgg caaatgcata
    23281 gcgttcttcc actttaaagc gtgtttccga cggtttgcct tcctgactca cacgcacgat
    23341 acgttcgccg ctttgcagaa tatttttcaa taacggcgcc tgaacgctct tcacatgcga
    23401 ctgccactga ccgcgcacca gcgccaggta atctttttgc atcccttttt cacgtaattg
    23461 ctcatgcaga gaacgcaacg ccgagcgttt tttcgctacc agcaacacac ctgaggtgtc
    23521 ccggtcaaga cgatgaacca gttcaaggaa ccgcgcttcc gggcgcaacg cccgcaaacc
    23581 ttcaataacg ccgaagctta aaccactgcc gccatgtacc gccgtaccgg aaggtttatt
    23641 cagcaccagg atgtgatcat cttcatataa gatgacgtcc gccagcgccg ccaccttttg
    23701 cagatgtggc gaaaccgcct cttcttcccg ctcagcaacg cgaaccggtg gaatacgcac
    23761 ctcatcaccc gcttcgagtt tatattcagg cttaatacgt tttttgttca cccgcacttc
    23821 gcctttacgc aaaatacggt aaatcatact ttttggtacg cctttcaatt gggtacgcaa
    23881 aaagttgtcg atacgttgcc ccgcttcgtc ggcggtgata gcaacaattt ttacggatgg
    23941 agtctctgtt ttcatggtgg gcgattctaa ttagccaaca ggattcgcgc cactcatttt
    24001 tctatgctta tatttagttt tgcaccttat tacttcacta cgtgatcact ttattgatgg
    24061 ttattaaacc aatcaccagc aagaagtgaa aaaactgtga gtaagcgggt gataaatggt
    24121 aaaagtcatc ttgctataac aaggcttgca gtggaataat gaggccgttt ccgtgtccat
    24181 ccttgttaaa acaagaaatt ttacggaata acccattttg cccgaccgat catccacgca
    24241 gcaatggcgt aagacgtatt gatctttcag gcagttagcg ggctgcgggt tgcagtcctt
    24301 accggtagat ggaaatattt ctggagagta atacccagtc tgtttctttg ataattgcgc
    24361 tgtttttccg catgaaaaac gggcaaccga cactctgcgc ctctttgagc tgacgataac
    24421 cgtgaggttg gcgacgcgac tagacacgag gccatcggtt cacacccgga aaggcgttac
    24481 tttgcccgca gcttagtcgt caatgtaaga ataatgagta agttacgatg aaaagaatgt
    24541 taatcaacgc aactcagcag gaagagttgc gcgttgccct tgtagatggg cagcgtctgt
    24601 atgacctgga tatcgaaagt ccagggcacg agcagaaaaa ggcaaacatc tacaaaggta
    24661 aaatcacccg cattgaaccg agtctggaag ctgcttttgt tgattacggc gctgaacgtc
    24721 acggtttcct cccactaaaa gaaattgccc gcgaatattt ccctgctaac tacagtgctc
    24781 atggtcgtcc caacattaaa gatgtgttgc gtgaaggtca ggaagtcatt gttcagatcg
    24841 ataaagaaga gcgcggcaac aaaggcgcgg cattaaccac ctttatcagt ctggcgggta
    24901 gctatctggt tctgatgccg aacaacccgc gcgcgggtgg catttctcgc cgtatcgaag
    24961 gcgacgaccg taccgaatta aaagaagcac tggcaagcct tgaactgccg gaaggcatgg
    25021 ggcttatcgt gcgcaccgct ggcgtcggca aatctgctga ggcgctgcaa tgggatttaa
    25081 gcttccgtct gaaacactgg gaagccatca aaaaagccgc tgaaagccgc ccggccccgt
    25141 tcctgattca tcaggagagc aacgtaatcg ttcgcgcatt ccgcgattac ttacgtcagg
    25201 acatcggcga aatccttatc gataacccga aagtgctcga actggcacgt cagcatatcg
    25261 ctgcattagg tcgcccggat ttcagcagca aaatcaaact gtacaccggc gagatcccgc
    25321 tgttcagcca ctaccagatc gagtcgcaga tcgagtccgc cttccagcgt gaagtccgtc
    25381 tgccatccgg tggttcgatt gttatcgaca gcaccgaagc gttaacggcc atcgacatca
    25441 actccgcacg cgcgacccgc ggcggcgata tcgaagaaac cgcgtttaac actaacctcg
    25501 aagctgccga tgagattgct cgtcagctgc gcctgcgtga cctcggcggc ctgattgtta
    25561 tcgacttcat cgacatgacg ccagtacgcc accagcgtgc ggtagaaaac cgtctgcgtg
    25621 aagcggtgcg tcaggaccgt gcgcgtattc aaatcagcca tatttctcgc tttggcctgc
    25681 tggaaatgtc ccgccagcgc ctgagcccat cactgggtga atccagtcat cacgtttgtc
    25741 cgcgttgttc tggtactggc accgtgcgtg acaacgaatc gctgtcgctc tctattctgc
    25801 gtctgatcga agaagaagcg ctgaaagaga acacccagga agttcacgcc attgttcctg
    25861 tgccaatcgc ttcttacctg ctgaatgaaa aacgttctgc ggtaaatgcc attgaaactc
    25921 gtcaggacgg tgtgcgctgt gtaattgtgc caaacgatca gatggaaacc ccgcactacc
    25981 acgtgctgcg cgtgcgtaaa ggggaagaaa ccccaacctt aagctacatg ctgccgaagc
    26041 tgcatgaaga agcgatggcg ctgccgtctg aagaagagtt cgctgaacgt aagcgtccgg
    26101 aacaacctgc gctggcaacc tttgccatgc cggatgtgcc gccagcgcca acgccagctg
    26161 aacctgccgc gcctgttgta gccccagcac cgaaagctgc accggcaaca ccagcaactc
    26221 ctgcacaacc tgggctgttg agccgcttct tcggcgcact gaaagcgctg ttcagcggtg
    26281 gtgaagaaac caaaccgacc gagcaaccag caccgaaagc agaagcgaaa ccggaacgtc
    26341 aacaggatcg tcgcaagcct cgtcagaaca accgccgtga ccgtaatgag cgccgcgaca
    26401 cccgtagtga acgtactgaa ggcagcgata atcgcgaaga aaaccgtcgt aatcgtcgcc
    26461 aagcacagca gcagactgcc gagacgcgtg agggccgtca gcaggctgag gtaacggaaa
    26521 aagcgcgtac cgccgacgag cagcaagcgc cgcgtcgtga acgtagccgc cgccgtaacg
    26581 atgataaacg tcaggcgcaa caagaagcga aggcgctgaa tgttgaagag caatctgttc
    26641 aggaaaccga acaggaagaa cgtgtacgtc cggttcagcc gcgtcgtaaa cagcgtcagc
    26701 tcaatcagaa agtgcgttac gagcaaagcg tagccgaaga aacggtagtc gcaccggtag
    26761 ctgaagaaac tgtcgctgcc gaaccaattg ttcaggaagc gccagctcca cgcacagaac
    26821 tggtgaaagt cccgctgcca gtcgtagcgc aaactgcacc agaacagcaa gaagagaaca
    26881 atgctgataa ccgtgacaac ggtggcatgc cgcgtcgttc tcgccgctcg cctcgtcacc
    26941 tgcgcgtgag tggtcagcgt cgtcgtcgct atcgtgacga gcgttatcca acccaatcgc
    27001 caatgccgtt gaccgtagcg tgcgcgtctc cggaactggc ctctggcaaa gtctggatcc
    27061 gctatccgat tgtacgtcca caagatgtac aggttgaaga gcagcgcgaa caggaagaag
    27121 tgcaagtgca gccgatggtg actgaggtcc ctgtcgccgc cgctgtcgaa ccggttgtta
    27181 gcgcgccagt tgttgaagaa atggctgaag tcgtagaagc ccccgtaccg gttgccgaac
    27241 cgcaaccgga agtggttgaa acgacgcatc ctgaagtgat tgccgccgcg gtaactgaac
    27301 agccgcaggt gattaccgag tctgatgttg ccgtagccca ggaagttgca gaacacgcag
    27361 aaccggtggt tgaaccgcag gaagagacgg cagatattga agaagttgcc gaaactgctg
    27421 aggttgtggt tgctgaacct gaagttgttg ctcaacctgc cgcgccagtc gtcgctgaag
    27481 tcgcagcaga agttgaaacg gtaaccgcgg ttaaaccaga gatcaccgtt gagcataacc
    27541 acgctaccgc gccaatgacg cgcgctccgg caccggaata tgttccggag gcaccgcgtc
    27601 acagtgactg gcagcgccct acttttgcct tcgaaggtaa aggtgccgca ggtggtcata
    27661 cggcaacgca tcatgcctct gccgctcctg cgcgtccgca acctgttgag taataattag
    27721 ctcaagtaat caagccctgg taactgccag ggctttttta tttcatcttt gaatcaacat
    27781 attaaaattc atcgaatggc atccttgcta accaacaatg caaaataggc aaaaaaatgc
    27841 ccgccccggc gatccctgag cgggcagaca aaacataccc agtttcatga tatgttttaa
    27901 agcgagctta tttgttgagc tggaagagcg acaatccctg catatcggta aatgctttat
    27961 acgatgcctg caatgccgtt tgctgcatga tgtaagatga aatagttgca ttccagtcca
    28021 catcaaccag atcgctcatc tgctgcgttt gccctaatgc gcgatcgcta cctaatgaat
    28081 ccagcgactc cagttcgttc agctgcgtgc ctaattccgc gcgaacggtc aggacattgt
    28141 tcagcgagtt tttcagtccg cggttggttt tatctaacgc cgcagcggcg gtttctttat
    28201 ccgcttcgct atccgcgacc ggcgttttca gggctgcgat ggcgctatcc agcatggcaa
    28261 aaagattggt ttcagaagcg ctaccgtctg gttccgctac cgcgttactg gtaatactgt
    28321 cgaaaatttt gtcgcccgta tgtcctatca ccatcgaacg cgaagcatcg acctgttgct
    28381 taatactttc tgttccgccg acataatcac cgttaacctc actaaacggc gcagtctctg
    28441 ttttataacc ggcaaaaatg tagcgcccgt taccgtcagt ggtgttcgcc agattcagca
    28501 actggtcacg aagcccctga atatccgtag ccagcgaggc ccggtcattg tcactcaagg
    28561 tgccattgct ggcgtagaca attttttcct gagcattctg gatagcggtg gtgacctggc
    28621 taagtacact ctcttccagt gacacttttt gagtggcgaa agtacgtgcc agcgtgtact
    28681 ggctgttttg cgcctgtgcc tgggagagaa caacagcttg tgatgcagca atgggatcgt
    28741 ctgaagggtt aacgactcgc ttacccgtcg acatctgctc gccgtacttc atccattctg
    28801 cctgagaatt ggtgatgccg cgcatgtttt gctggtacat catctgggta ctgaaacgca
    28861 ttttatctcc ccttagcgaa tgttaatcag cgcatcaaaa atcgcgtttg ccgtctgcag
    28921 aacctgcgca ttcgccaggt aatactgctg aaaacgttgc agatttccgt actcctcatc
    28981 gagattgaca ccggaaatcg actgctgctg attggaaagc tgcgtcacca cattaccttg
    29041 cgtggtgcta ctggttttca acgtcgcggt tttattaccg atatcactca ctaacgaggc
    29101 ataagcgtcg ttaaaggatt tcgctccgcc caccgtttta ctgttgcttt gcagatccag
    29161 cagggcctga ccgttgcggt tgtcgctatc acccgcatct tcttcgctcg ccatcgctat
    29221 tttcgcttcg tcggtgatta atacatccat gttgacgatg gcgtcactta ctggtttcag
    29281 cgtgaagctg tcgttaacgg caggcgttcc tgtaaacgtc aactccagac catcaaatgc
    29341 cactttaccg ttggcatctg gcgtcaccgt aaaagtggta ttgctggcaa ggcgggtgac
    29401 ctgccactga ttattatcga acgagatttt gtaatctgtc gccagtaccg cggaggcatc
    29461 agttaccgtg gcaccgatcg caacgttacc gttgttttta gtgttttgca gaaccgcggg
    29521 cttaccgata gcaaagaaat cttcaccctc atcgccgttg gcatcaaatc cggctttgtg
    29581 ttggctgttg aaagcctcag caaatgccag cgccagttgt ccaagcgtat tacgcgtctg
    29641 gtccagatcc tgagaacgga atgtcagaat gccgcccagc gacccggtat tcagtaattt
    29701 ctccgggatc tcaatattgc ctgccgtccc atcaacataa gcgacagtcg tacgagaagg
    29761 gtcggcgctg gaaggaactg ccgccagttg ccgcgccgta cttccctgaa ccagtgagta
    29821 accattggcc atcgtgatgt tataagtgcc gccatcctga acgctgactt ctacaccaac
    29881 aatctggttt aattcgctca ccagttgatc gcgttgatcc agcagattgt taggtgacgc
    29941 ccctgccccc acgcctgtca ggcgggagat ttgatcgttc aggctggcga tttgtttagc
    30001 gtaattgttg atctgatcaa cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc
    30061 gcgaagatac tgatcggtgg ttttaaactg attcaccaac ccttctgatt tcccaatcag
    30121 cgcctggcgc gctgccgggt cttccgcgtt actcaccagc gtttgcaggc tggtgaagaa
    30181 atcctgcatc tgtgttgcca gcgaagaggt actggtggag agcatattgt cgattttcga
    30241 catctgctca tagcgggcag tcaggccgct actttgcgtc tgcgccgcac gtaactggtt
    30301 ggtaataaac gcatcatact cacgctgcac accagaaacg tagacaccat taccaaccca
    30361 gccgccagcg cccaacgtgc tattggcctg cgccataata gtggtttggc gggtatatcc
    30421 ggcaacgtta tagctggaga tattattact tgccgtattt aacgccgcct gggccgcgtt
    30481 cagtccgctc atggcgttat tgatcaagct ggacatggag gttccttata agccttcaat
    30541 tacagagtat tatcggcagc gacccgccgg acttgagtta ttcagaacag attatcaatg
    30601 ttcatgctgt aggttttgct caccttgtcg cttatcgatt tcatctgctg aatcatgttg
    30661 gtgagtttac gggcatagtg aggatcggtg gcatagcccg cgtcctgtag ggcctgcgcc
    30721 ccctgctccg cactcgcggc ggtcgtcacg gcggcgtagc gcgggttacg tgttaacagc
    30781 ccaacgtaat ccgacaatgc ttccagatac gagctgtaga cccgaaactt cgcttttact
    30841 ttcttcgctt cgccattttc atattcagtc gtggtgattt cagtgactgg ccctttccag
    30901 ttgccagagg ctttgacacc aaacaggtta tagctcggct cgccgttttc gcggcggatt
    30961 tgccgttgcc cccagccaga ttccagcgcc gcctgagcga ggatcaaatg atgtggcaca
    31021 ccgctttgct ggcttgccag ttgggcgggc agcgagagtt gcgcgaggaa tgctttactg
    31081 tcacctggca gcgaatcatc gtagttacgt ggcacggcct tttgcaccag ctgcgaaagc
    31141 gcctgatttt gataacgcac cacggtttcg agcgggaatt tcatcggtgc tgctggcgtg
    31201 gactcctctg gcaatggttg ttctggcgtc atctgcttaa ccatcatctc tgcaagcccc
    31261 agacctttgc ccgtcgtcat ctgttgggca atctgctggt catacatact ggtatacagg
    31321 cgagtgtgct cgctgctgaa caggccatct tttggtaacg cgtcgcgcat gcttttcaac
    31381 atcatctgca cgaacatccc ttccacctga cgggccaccg gacggatatt tgccgccgga
    31441 tcttcgctcg ctttcgcctt tagttcgttg agtgattgcg catcccaggc cgcacttgcc
    31501 agtagtttgc tgtcgctgat catcagatga tttccagttt tgcccgcaga catcccgcac
    31561 tttgcattga ttgcaaaata gacatcagat ccatcggcgt agcgcccagc gcattgagcg
    31621 cgcgcaccac gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct
    31681 ggcgtaagtc gatctgcgtt tgtggcgtta ccacggtctg tccgccacca aacggtgtat
    31741 ctggttggct gacattggcc tgacgattga ctgttactga gagatttccc tgcgctatcg
    31801 cgcagctgtc gagagtcact tcgcgattca tcaccaccga accggtgcgc gagttaatca
    31861 ctactttagc gtcctgcggg gtgacattaa cctgcatatt ctggatatcg gcaaggaaac
    31921 ggacctggga actgttgcca ctcggtacgc gcacctgaat agtccgcgca tccaacgcgg
    31981 tggcgctgcc atatccacgc acgcggttga tggtgtcagc gatttgctgc gccatgctga
    32041 aatcttcgtc gttaagttgc aaattaaggg tattcccgac gccaaactgg ctgggtaatt
    32101 cacgttcaat aactgcacca ttggtgatcc gtccaccgtt cagttggttc acctgaacac
    32161 tgctaccgcc agcggaggct cctgcgccgc caaccagaat attgccctgc gccagcgcat
    32221 acacctgact gtcaacgccc ttaagcggag tcatcaacaa tgtgccgcca cgcaggcttt
    32281 tggcatttcc catggaagaa accaccacgt caatggtttg cccctgacgt ccaaacggtg
    32341 gaagtgacgc cgtcaccatt accgcagcaa cgttttttag ctgcatattg gtgcccgtcg
    32401 gaacggtaat tcccagctgt gagagcatgt tattaagcgt ttgtgtggta aacggcgtct
    32461 gggttgtctg gtcaccggtg ccatccagcc ccaccaccag accatagcca atcagtgagt
    32521 tttgccttac cccctgaaca ctggtgagat cgcgaatacg ctcagcctga gccgccgtcg
    32581 taaccagtag aagaattaat gcagagagaa atttaatcac cacagcctca cttacattgg
    32641 cgacaggtta aggaagaaac gctgcaacca gcccatattt tgcgcttcgt taatgtagcc
    32701 attgcctacg tattcaatgc gcgcatccgc cacctgagta gacggtacgg tattgctgcc
    32761 gctgatagtg cgtggattaa ccacgccaga gaagcgaata aattcggtac cctgattaat
    32821 ggcaatctgt ttttcaccca ccacatgcag gttgccgttg accagtacct ggtcaaccgt
    32881 caccgtcaac gtgccgctaa aggtattact ggcattggcc ccgccttttc cgttgaacgt
    32941 gttaccaccg gaggcttcga catcggcacg agcgttacca aacagcccct gcaaatagcg
    33001 cggcacagta tcaaagccaa aattagtttt accgtcacgg ctggcattcg cagaggagct
    33061 tttgctggcg ctaacgttct cctgcaacac gatggtcagc gtatcgccaa tattgcgtgg
    33121 tcgacgatct tcaaacagcg gttgatagcc atagttaatc ggctgagcag actggaaaat
    33181 agaaccgttg gcgacgggcg tcggaccggg aaccggttgt gcactggtcg ccccctgcac
    33241 cagcggcgtg gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca agctggaaat
    33301 ggcataagta tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc aataaaccgg
    33361 tgaacctgcc accggttatg ccttagagtt gcgtcagttt ttgcagcatc tgatcggtgg
    33421 tggacaccgc tttactgttg atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt
    33481 cttccgccac gttgacgtta gacgtttcaa catacccttg atacagcagt cccgcgccgt
    33541 tcaggcccgg cgtgctttcg ttcggtgcac cggaggattg cgtttcggtg tagaggtttt
    33601 cgccaatgct ctccagcccg gtgtcattca taaaggtggt gagattgagc tgcccaacct
    33661 gaaccggagc tgcctggcct tgttgggtta cgctgaccac gccatcacga ccgatggtga
    33721 tacttaacgc attcgccgga atggtgatcg ctggctgcac ctgaaaacca ccagccgtca
    33781 ccagctgccc gttctgatcc acctggaaag agccgtcacg ggtataggct gatgagccat
    33841 ctggcaacat cacctggaaa aagccctgcc ctttaatcgc gacatctttg ctgttgttgg
    33901 tctgcgacag gttgccttgg ctgtgtaagc gttcagtggc gaccgggcgt acccccgtgc
    33961 cgatttgtaa tccggagggt aaggtggttt gttcggaaga ctgtgccccc ggctggcgaa
    34021 tggtttgata aagcagatct tcaaacaccg cgcgctgacg cttaaaaccg ttagtactga
    34081 cgtttgccag gttgttggca atgacgtcca tattggtttg ctgggcgtca aggcccgttt
    34141 tggcgatcca taatgaactg atcataaaat gtcctgtagt ggattgctga caagcagagc
    34201 agaagcacgc ggcggtgcga atttcccctc accctaaccc tctccccaaa ggggcgaggg
    34261 aacagtccgc tcgcttttat tgcaatgtca tcaatcgttc aattaactca tcgacagcag
    34321 ttggttggca cggcctgcgt tatcatcgac gctgctgatc accttcatct gcatttcaaa
    34381 acgccgcgcg ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt tactgccttc
    34441 cagaaccccc gacatcacac gcaaggttgg atctgcctgc agtaccggtc cacgcgtggc
    34501 ctgggtttct gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt
    34561 ggctttcacc agtttcagac gccccactgg cgcaaccgta tttgccggat cgcccggatt
    34621 cagcgccgag attgttccat cggcagctat cgtgatttcc gccccttccg gcacagcaat
    34681 tggccccgcc tcgcctatca ccggatgccc ctgaattgtc agttgcccgg tgggatcaac
    34741 ctgaatgctg ccattacgcg tatacccttc gctgccgtca gcggtctgca cggccaacca
    34801 gccatcctgc tgcaacgcaa cgtccagcgg gcgcgaggtg taatccatct tgccgggcgt
    34861 tatgtctgca cccggtgttg acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc
    34921 caccggcact gcgcgcaagg cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc
    34981 cagattactg gcggttaccg cctgttgatt cagcgtctgg ctggctgctc ccatcgcggt
    35041 ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta accagcgtgt
    35101 tgaggatctg gtcctgggtt ttgatggtct gggcgttaga ctgatagtta cgctgggcaa
    35161 cgatcatatt gaccagttct ttactgagat cgacgttgga cgcttccaac gcaccgttgg
    35221 tcagggtgcc aaagttgccc gtcccggctg tccccaacag cgccacgcca gaagattgcg
    35281 tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca aagttcgcca
    35341 gtacaatctg ccccagcagt tgggtttgtt cgttggaata gttgccgaca accgtaccgt
    35401 catcattgat ttgataactc accagatcgc ccggtttgta gccattctgg gtggttgcca
    35461 caatgttgtt agcgccggta ttttgctgca tggagttgag gaagctcagg ctaaacgtgg
    35521 cgggttctgc gccgttaatt gcgccggtgg taatattctc tgttggattt gaggtcagaa
    35581 cgccattggc attaaacacc agcttcattg caggctcggc tgtacctgtt ggatcactgc
    35641 tatcctgggt gtagacctgc cagttattat ccccggtctt cacaaagtag acgctcatgt
    35701 catgagcatt accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatccg
    35761 cattgctggc atcaaatgcg ttaacagagg gaagcggatc gctggaattc aggttgatct
    35821 gcatcgacgc cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatgttag
    35881 tcggattcgc cccttgctga atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct
    35941 gtaaaccttg catattcacc aggttacgat tttcatccag cttaaattgt ccgttacggc
    36001 tgtagaacac cgaaccgttg ctatctacca gacggaaaaa accgttctgg ctgatagcaa
    36061 cgtccagacc acgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg
    36121 caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc
    36181 ctgatttaaa gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg
    36241 tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg
    36301 tgaaggctta aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat
    36361 ccagcgtatt accgttgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct
    36421 gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacgttgtaa gaaccgttcg
    36481 gcgcagtgct gccgtcagtc aacgtaccgt cccaggtgaa actgtgaact ccggcggtca
    36541 gttcaccaat atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa
    36601 ctttgtctgc cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt
    36661 cttcactgcc ggttcccgca agaacagtgg tgccggggat catcacgccg tgaccgatca
    36721 ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg
    36781 tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt
    36841 tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccaac aaagtcagaa
    36901 aactgctttg taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga
    36961 cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg
    37021 accgagcgta agggttttca gcatcatgct tttcaccgtg ttgagcactt caacattggc
    37081 ctgatagctg cgtgacgccg acatggtgtt aaccatctct ccgacaacat caacgttcgg
    37141 catttttacg tagccctttg catccgccag cggattaccc ggttcataaa ccagtttgtc
    37201 cggggcctga ctttctataa catcggcaac ctttacgccg cctgtcgcag cacctggtgc
    37261 agcgttaacc tggaatacca cctgttttgc ccgatatggc tgtccatcag gaccggtcac
    37321 gctatcagca ttcgccagat tactggccgc cacgttcagg cgctgggact gggcagttaa
    37381 cgccgacccg gcgatatcaa aaatattcag cagtgccatc cgttaatttc cgctctgtaa
    37441 aacgttcatc atgcctttga tttgcccgct caacgcgcta aggctcatct ggtattgcag
    37501 gctgttatcg gcaaactggg tgcgttcgcg atccatatcg acggtattac cgtcaagcga
    37561 aggctggtcc ggaatacggt attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg
    37621 aatgtgttgc gttgaggtca tcgtcagagc aaccacactg gttgcattcc gtccacgttg
    37681 catgactttt ttaagttcac tggcaaaatc gatatcgcgc gcctgataac caggggtatc
    37741 ggcattggcg atgtttgctg ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc
    37801 ttgttgaaaa cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc aggtatcaaa
    37861 attctgccat cagcttaaat gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg
    37921 ggtcgctatt tatgccgttg atggtcattg tggacaggta caattcacgt tgtagaaatg
    37981 gctgggggcg aaaatgctgg caataaaacg tagcgtggcg atcatcgcga tactgttcag
    38041 tccgttaagt gcggcgagca atctcacatc gcaattgcaa acctttttta gctcccagct
    38101 cgcagggata agtgatgagg ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc
    38161 gtgcgagcag ccattgcttt cgatgagcaa taattcccgc ttgtggggca atgtgaatgt
    38221 gttggcacgc tgcggtaacg acaaacgata tttacaggtt aatgtgcagg ccacaggaaa
    38281 ttatgtggtt gccgcgatgc ccattgtgcg aggaggaaag ctgggagctg gcaatgtcaa
    38341 actgaaacgc ggacggctgg ataccttgcc accgcgtacg gtgctggata tcaatcaact
    38401 tgttgatgcc gttagcctgc gcgatctatc acccgatcaa cctatccagt taacccagtt
    38461 tcgccaggca tggcgggtaa aagcgggaca acgcgtcaac gtgatcgcca gcggtgatgg
    38521 gtttagcgcc aacgcagaag gtcaggcgct aaacaatgca gccgtcgcac agaatgcgcg
    38581 ggtgcgcatg gtatctggac aggtagtcag cggcgttgtt gatgcagatg ggaatattct
    38641 tataaacctg taagctgtta aagattaccc gtcccttgcc gataaataag caacacatga
    38701 taaaagcgcc ctcaatgagg aataaaccat gagtattgat cgcacttcgc ctctgaagcc
    38761 cgtaagcacc gttcaaccgc gcgaaaccac tgacgcgccg gtaacgaaca cccgagcggc
    38821 aaaaacaaca gcctccacca gcaccagtgt gacgttaagc gacgcgcaag caaaactgat
    38881 gcaacccggc agcagtgata tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg
    38941 taacggtgaa ctaaaaatgg acaccggcaa aattgccgat gcgctgatca acgaagcgca
    39001 gcaagacttg cagagtaatt aatcgtatga cgcgtcttgc agagatcctc gaccagatgt
    39061 ccgctgtgct taacgatctc aaaacggtaa tggatcaaga gcagcaacat ctctctatgg
    39121 ggcagatcaa cggcagccag ttgcaatgga ttacagaaca aaaaagctca ctgctggcaa
    39181 cgttggatta cctcgaacag ttacgcagga aagaacccaa tacagcaaat agcgttgata
    39241 ttagtcaacg ctggcaggaa attgctgtga aaacgcagca actacgccaa atgaatcaac
    39301 ataacggctg gttactggaa ggacagattg agcgcaatca acaggcgctg gaaatgttga
    39361 aaccgcatca ggaaccgacg ctatatgggg cgaacggtca gacctcaaca acccatcgcg
    39421 gcagtaaaaa gatttcgatc tgaagattat ctccggcctg cactgcaggc cggaatgcat
    39481 tgttacaccg tccggcgggc aaattcttta actttgaagc ccagtaccgc cagtgcagcg
    39541 aagtacgcgg caatccccgc cagcacgacc gccattaaac gcagcaaacg ccagggcatg
    39601 gtacccaatg accactccgg catgatatgt aacataccta aaagcacgcc agacatcacc
    39661 agtaccgcca ccaccagacg caacagaaac gccatccagc caggttgcgg ggtaaagatt
    39721 ttctgcttac gcaactgcca gtaaagcagc gaagcattca gacacgccgc cagaccaata
    39781 gaaagtgaca gcccggcatg tttcaacgga ccgataaacg ccaggttcat caattgcgtt
    39841 aaaatcagcg taacgatggc aattttcact ggcgttttaa tgtcctggcg ggaataaaag
    39901 ccaggagcca acactttcac tacaatcagg ccaatcaaac ccaccgagta ggcaattaac
    39961 gcccgctggg tcatcagcgc atcaaaggcg gtaaatttac cgtactggaa cagcgaaacg
    40021 gttaacggac cggaaagaat gcccaacgca accgcactcg gcaacgccaa caggaaacaa
    40081 agacgcagtc cccagtccat caaacggttg tattcatcat gattgccact ggcaaaactt
    40141 ttcgacagcg acggtagcaa aatggtgcca agcgccacgc ccagcacacc ggacggaaac
    40201 tccattaagc ggtcggcgta atacatccat gacaccgaac cggaagcaag aaacgaggca
    40261 aaaatggtgt tgatgattaa ggagatctgg ctcacagaga cgccaaggat tgccggtccc
    40321 atctgtttca ccacacgcat tgctccggca tcgtggaagt taatgcgtgg caaaaccagc
    40381 atgccgatct tcttcagatg cggtagctga tacaccagct gcaggacgcc gccgaccgtc
    40441 acagcccacg ccagcgccag caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa
    40501 ccaatcatgc tgatgttaag cagtgttgga gcaaacgccg gaatcgagaa gcggttccac
    40561 gtattcagaa tcgctcccac cagcgacgcc agggagatca gcaagatata gggaaaggta
    40621 atctttagta gctggctggt cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg
    40681 gtcaccatga tcacccacgg tgcggcgagc atgccagcga ccgtcacaac cgccagcgca
    40741 agtgtcagca ggccagaaac ataagagaca aagacccgcg tggcgtcttc accctgctta
    40801 cttttatatt ccgccagaat cggtacaaat gcctgggaaa atgccccttc ggcaaagata
    40861 cggcgtaaca agttaggaag tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg
    40921 ccaaagattc tggcgacaat tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc
    40981 atcgagctga cggcggccag cgattttaat aaattcatcg gtgttctaat ccagacccac
    41041 gggcaaaacg cctgcaaggc aggcgctgaa agaggcgaat agtctacctg gattatggtg
    41101 aattgctacc gccagatgtt acagggttta ttcactcatc gcatcgcgcc agatcttgtc
    41161 aacgatacgt tgcgccagca ctgcctgttc gccggcggtt tgcggaactg tctgattttg
    41221 cacgcattca atgaagtgcc gcgcacagcc gacaaatcca cgttgctcaa gcgtgctctg
    41281 ccaaccagga atcggtttat tcactacacc ctgcccacgc tcctcacgcc attcgcgcat
    41341 ttccgtaatg tcgatgagcg caccgtcagt cacggcctgc acggtttcac gctgacttcc
    41401 ggcacggcga tgcatacaag tggtgatctg caaaggaccg gccgaaaaat ggtgctcggc
    41461 aaacagcatt tcgccagcgt cgttagtcag tagcgtaccg ccatccagag aggctttgcc
    41521 gcccgacaac cacagcgcgg tatccaccac atgcagataa tcatccagca acgtgaaata
    41581 aagatcgtgt ggcccgacgc tattgctacg atgtttatcc attcttagcg aggctgcggt
    41641 ggcgagttgc gtttttaact cacagtagag tggtgcgaaa cgacggttaa aaccgaccat
    41701 caacgtcagt tttttccgcg ccgccagttc caccagccgt tcagcatcgc gcagattttc
    41761 tgccagcggt ttatcgacac agacatgtac ccccgcattg agtaacgtac tgaccacgtc
    41821 aaagtgactg gcggtgctgg aatgtacaaa aaccgcatcg caactggcgg caaggctgga
    41881 taacgaatcg gcataaggaa tgcgccagct ttcacaaatt ggcagggctt tcgcgcgcgt
    41941 aggcgaccag gctccttgta acgtccagtc agacgctgcc gccagcaccg gtaaccacgc
    42001 tttttgcgca atgccgccta atcccactac gccgatacgt aattttttca ctttaatctc
    42061 ccaggtgggc cagcaacgaa tcaagacgct gtttcagttc tgccacttcg atttccaggg
    42121 cttcgacgcg ggcctgtaaa tcaccgtcaa ccgcgttcga catatccgtt accgccggct
    42181 gatcctctac ctcaccactg aaaagatgca tgtagcggct ttcgcgttta cccggttcgc
    42241 gggccagacg caccacaaaa ggaccatctt cgcgattcgc cagttgttcc agcgtcaact
    42301 ccacttccgc catatcgctg aattcataca ttcgcgcggc gcggctgcgc agttcacccg
    42361 gcgtctgggc accacgcaat aacaacgtgg tgattaacgc cacttctgcg gcgctcagtt
    42421 tcagatcgcc aaattctgaa ttacaaaaac gttgctcata tttggtgacc cgattaccaa
    42481 aaccgctcac tgtgcgtaga taatgacgtt tgaccagatt atccagctgt tcctgcactt
    42541 cagattcact cagattcatg accggttcac ggttcgtttt ctgattacag gccgtgacta
    42601 caccattgac tgagagcggg tattgctccg gcgtggtcac ctgcttttcc agcaagcaac
    42661 caatcacacg agcttccagg gcagtaagtt gatatttcat gagattctcc ttagcggccg
    42721 ggcgtccagt ctggggtagt taatgccgtc agtacgtgat cgcgccattg tccatcaatc
    42781 aacagatagt ctttcgcata gccttctttt tcaaaaccca gtcgcgccag taaatcaccg
    42841 ctgcgtttat tgtgcggcat ataattagcc ataatgcgat gaatatgttg ggtgcgctgc
    42901 atataacgaa tggctgcggt cagggcttca aacatgagtc ctttgccctg ccatttttgc
    42961 ccaatcgaat aaccgagata gcaggcatga aaagagccgc gaacaacatt ggaaaaattg
    43021 gcaacgccaa taatctcttt ttcgtccggg tcgaataagc caaagtagaa agctgaacct
    43081 tgtttatgaa attcgttaat catccccagc ctggcctgcc agcctgatgg ataacagtgg
    43141 ctttcgtcgc gcactggctc ccagggcttg aggaaatggc gattctctgc gtaataatcc
    43201 gccagacgcc aggcatcacg atcatgcacc agacgcacga ccagtcggtc tgtggttaag
    43261 cgcacttttg gcacgttact gcgatagcca aacattctat acctactcct tcccgtaacg
    43321 tctttaatac gctgtaagta ttactatacc tgcgcaacca gaccctgtga aaagtccaaa
    43381 atgccatttt ctgcattatt cacatatttg ataaaaactc tctatcaaag cgacgttttg
    43441 ataaaaaaat attgtccccg aagtgatggg aaaagtctta gcggcaatgc agaatagaaa
    43501 gtgctcatct tttatttccc gggaggggaa atgtcccgcg tgtcgcaggc gaggaacctg
    43561 ggtaaatatt tcctgctcat cgataatatg ctggtcgtgc tggggttctt tgttgtcttc
    43621 ccgctgatct ctatccgctt cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt
    43681 gccctcggcc tacgccagtt tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc
    43741 gaccgctttg gtgccaaacc gatgattgtt accggtatgc tgatgcgtgc cgccggattc
    43801 gccacaatgg gtatcgccca cgaaccgtgg ctattgtggt tttcatgcct gctctcggga
    43861 ctcggtggca cgttgtttga tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca
    43921 caacagcgtg gtcgtttttt ctcgctgttg atgatgcagg acagtgccgg tgcggtcatt
    43981 ggcgcattgt tggggagctg gctgttgcaa tacgactttc gcctggtctg cgccacaggg
    44041 gccgttttgt ttgtgctgtg tgcagcgttc aatgcgtggt tgttaccggc atggaaactc
    44101 tccaccgtac gcacgcccgt tcgcgaaggc atgacccgcg tgatgcgcga caagcgtttt
    44161 gtcacctatg tcctgacgct ggcgggttac tacatgctgg ctgtacaagt gatgctgatg
    44221 ctgccaatta tggtcaacga tgtggctggc gcgccctctg ccgttaaatg gatgtatgcc
    44281 attgaagcgt gtctgtcgtt aacgttgctc taccctatcg cccgctggag tgaaaagcat
    44341 tttcgtctgg aacaccggtt gatggctggg ctgttgataa tgtcattaag catgatgccg
    44401 gtgggcatgg tcagcggcct gcaacaactt ttcaccctga tttgtctgtt ttatatcggg
    44461 tcgatcattg ccgagcctgc gcgtgaaacc ttaagtgctt cgctggcgga cgcaagagct
    44521 cgcggcagct atatggggtt tagccgtctg ggtctggcga ttggcggcgc tattggttat
    44581 atcggtggcg gctggctgtt tgacctgggc aaatcggtgc accagccaga gcttccgtgg
    44641 atgatgctgg gcattattgg catcttcact ttccttgcgc tgggttggca gtttagccag
    44701 aaacgcgccg cgcgtcgttt gcttgaacgc gacgcctgat ttgatactcc cccctgactc
    44761 ctccatactg aaagtgaact gacagccaaa tggaggagtc acgtgaagct atacatttac
    44821 gatcactgcc cttactgcct caaagcccgc atgattttcg gcctgaaaaa tatccccgtc
    44881 gaattacatg ttctgctcaa cgacgacgca gaaacaccca cccggatggt cggtcaaaaa
    44941 caggttccca ttctgcaaaa agatgacagc cgctatatgc cagaaagcat ggatatcgtt
    45001 cactatgtcg ataaactcga cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt
    45061 gaagagtggc tgcgcaaggt caatggctac gccaacaaac tgctgttgcc gcgttttgcc
    45121 aaatcggcat ttgatgagtt ttctactccc gccgcgcgca aatatttcgt cgacaagaaa
    45181 gaggccagcg cgggtaattt tgccgacctg ctggcccact ctgacggtct gattaagaat
    45241 atcagcgatg atttacgtgc gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc
    45301 gaactttcgg aagatgatat tcagctattc ccgctactgc gtaatctgac gctggtagcc
    45361 ggaattaact ggccaagccg cgttgctgat taccgcgata atatggcgaa acagacacaa
    45421 atcaatttgt tatcatcaat ggcgatttaa tcctgactcg ccgcggcagc ctgtattcgc
    45481 gggttgccgc cgttttcagt tttttctgct ggcgagttta tcgctggtga cacatgctgc
    45541 gttgtgtcag gatattgacg cagatttcat gtcgaggact accatgaaca agtttttatt
    45601 tgccgctgca ttgatcgtca gtggcctgct cgttggctgt aatcaactca cccaatacac
    45661 catcaccgaa caagaaatta accagtcgct tgcgaaacat aataatttct caaaagatat
    45721 tggtttaccc ggtgtggctg acgcccatat tgttctgaca aacctgacca gccaaattgg
    45781 tcgcgaagag ccgaataagg ttgccctaac cggagacgcc aatctggaca tgaactccct
    45841 gttcggtagc cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa
    45901 agaaaaaggc gcgatcttcc tgaaagagat ggaggtggtc gatgcgacgg tacaaccgga
    45961 aaaaatgcaa acggtgatgc aaacgttgct tccctatctg aaccaggcat tacgcaatta
    46021 ctttaaccag caacctgctt acgtcctgcg cgaagatggc agccagggcg aagcaatggc
    46081 gaaaaaactg gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc catttactga
    46141 ttaatcacga gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt
    46201 atcctttgtg tccggcaaaa acatcccttc agccggagca tagagattaa tgactgcacc
    46261 atcccaggta ttaaagatcc gccgcccaga cgactggcac cttcacctcc gcgatggcga
    46321 catgttaaaa actgtcgtgc cgtataccag cgaaatttat ggacgggcta tcgtaatgcc
    46381 caatctggct ccgcccgtga ccaccgttga ggctgccgtg gcgtatcgcc aacgtattct
    46441 tcacgccgta cctgccgggc acgatttcac cccattgatg acctgttatt taacagattc
    46501 gctggatcct aatgagctgg agcgcggatt taacgaaggc gtgttcaccg ctgcaaaact
    46561 ttacccggca aacgcaacca ctaactccag ccacggcgtg acgtcagttg acgcaatcat
    46621 gccggtatta gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac
    46681 acatgcagat atcgacattt ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc
    46741 tctgcgccag cgcctgaccg cgctgaaagt cgtttttgag cacatcacca ccaaagacgc
    46801 agccgactat gtccgtgacg gaaatgaacg gctggctgct accatcactc cgcaacatct
    46861 gatgtttaac cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt attgtctacc
    46921 catcctcaaa cgcaatattc accaacaggc attgcgtgaa ctagtcgcca gcggttttaa
    46981 tcgagtattc ctcggtacgg attctgcgcc acatgcacgt catcgcaaag agagcagctg
    47041 cggctgcgcg ggctgcttca acgccccaac cgcgctggga agttacgcta ccgtctttga
    47101 agagatgaat gctctgcagc actttgaagc attctgttct gtaaacggcc cgcagttcta
    47161 tgggttaccg gtcaacgaca cattcatcga actggtacgt gaagagcatc aggttgctga
    47221 aagcatcgca ctgactgatg acacgctggt gccattcctc gccggggaaa cggtacgctg
    47281 gtctgttaaa caataaaaat cgtagcttcc tgttgtcatt aggttatttt acctgtataa
    47341 ataaccagta tattcaacag ggggctatta tgcgaattga agtcaccata gcgaaaactt
    47401 ctccattgcc agctggggct attgacgccc tggctggcga actttcccgc cgtattcagt
    47461 atgcgtttcc tgataatgaa ggccacgtat cagtacgtta tgccgcagcg aataatttat
    47521 cggttattgg cgcgacaaaa gaagataaac agcgcattag cgaaattctc caggaaacgt
    47581 gggaaagcgc cgatgactgg tttgtcagcg aataatatgc agtgattttt gtttttgccg
    47641 ggtcgccccg gcttttttat tttacttgcg aattatgagt acaaaatttc tacacttcct
    47701 caaaataatt gcacaatatg ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat
    47761 aaatcttaaa agcagaaatt tatctgttcc cccctttaaa aattcggtga ttacccttat
    47821 ttattggtat actgaagttg cttcagataa aaacgcattg aacctcgaat aacgttgtct
    47881 agtaacacga attagggggc catgatggaa aaaaataatg aagtcattca gactcatccg
    47941 ctcgtagggt gggacatcag caccgttgat agctatgatg cgctgatgtt gcgtttgcac
    48001 taccagaccc caaataagtc cgagcaggaa gggactgaag ttggtcagac gctctggtta
    48061 accactgatg ttgccagaca atttatttcg atattagaag caggaatcgc caaaattgaa
    48121 tccggtgatt tcccggtaaa cgagtatcgg cgtcattaac atgacatgac catccaccca
    48181 aaaggcacct caccggtgcc tttaccattt ctgggttgta tatctgcatt cagttaatta
    48241 ctctgcttaa accatgagag cagagagaaa aaaatgaaat acgatctcat cattattggc
    48301 agcggttccg taggcgctgc cgccgggtat tatgcaaccc gcgccggttt aaacgtgctt
    48361 atgaccgacg cccatatgcc accacatcaa cacggcagcc accacggcga tacgcgatta
    48421 attcgacatg cttatggtga aggcgaaaag tatgtcccgc tggtcctccg cgcacaaacg
    48481 ctgtgggatg acctctcccg ccacaacgaa gatgatccca tttttgtacg ctctggtgtc
    48541 attaaccttg gcccggctga ctccgcattt ctcgccaacg tcgcccacag cgccgaacag
    48601 tggcaactca acgttgaaaa gctcgatgcg caagggatta tggcccgctg gccagaaata
    48661 cgcgtcccgg acaactacat cggcttattt gagactgatt ccggtttttt gcgcagcgaa
    48721 ctggcgatta aaacctggat ccaactggcg aaggaagcgg gctgtgcgca actgttcaac
    48781 tgcccggtca ccgcaattcg tcatgacgat gatggcgtaa ctattgaaac ggctgacggt
    48841 gagtatcagg cgaaaaaagc gattgtctgc gcgggaacat gggtaaaaga cctgctcccg
    48901 gagctgcctg tccagcccgt acgtaaagta tttgcctggt atcaggccga tggccgctat
    48961 agcgtgaaga ataaattccc ggcgtttacc ggtgaactgc ccaatggcga tcaatattat
    49021 ggttttccgg cagaaaacga cgcgttgaag attggcaaac ataacggagg ccaggttatc
    49081 cattcagcgg atgaacgtgt tccgtttgcg gaagtggtca gcgatggttc ggaagccttc
    49141 ccgttcttgc gcaatgtatt gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc
    49201 tatgataatt cgcctgacga agattttatt atcgataccc tacccggcca cgataataca
    49261 ctgctcatta ccggcctgag tgggcacggt tttaaatttg cgtcagtttt aggggaaata
    49321 gctgccgatt ttgcgcaaga caaaaaaagc gattttgatt tgacgccatt cagcctttcc
    49381 cgcttccaat aatcatcatt acggcctcca gaatgaggcc gtttttacag gagtatttaa
    49441 tgcgtcggct gttgcactat ctcatcaata atattcgcga gcatctgatg ctatatcttt
    49501 ttctctgggg attgttggca attatggatc tgatctatgt gttttatttt tagttttccg
    49561 cactgacata aactgacaaa tatttaatct ccctttcatt tagcgtgaaa aattgaatga
    49621 gattattcct gcttctacag cactcaccag acatctctga tgctgaaccc actaaactta
    49681 tgaagattct ttcattatta attcaaaatt attgaaataa aaataaatat cattcaattt
    49741 gttatttttg tgttgcaatt cattctccga cacgccattt tctttctctc acaaattcgt
    49801 tttggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta tatctgcggc
    49861 cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc tgtggatggt
    49921 cacgctcagt tattacgatg gctggtatca caaagcaccc gaactacata aaagcatcgg
    49981 tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat ctccaccgcc
    50041 gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaagctg gacatctcgc
    50101 attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa ctgccgatgg
    50161 taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg acgctggcgc
    50221 acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg tagtactgtc
    50281 cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg acactctgaa
    50341 gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa aaagcctgct
    50401 tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg ccgattacaa
    50461 aattgacaaa gaaggtcagc acgcctttgt taactttcgc atccagcatc ttggctatag
    50521 ctggttatat ggcaccttta aagatttcga cggtactttt acctttgatg aaaaaaatcc
    50581 agctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta atcacgccga
    50641 acgtgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc cacaggcaac
    50701 attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg gcgatctgac
    50761 gctgaatggt gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc agggcgacga
    50821 cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc tcaaggactt
    50881 caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta tttcagtgga
    50941 aggcgtacag cagaagtaag agtgaaatgt tgccggatgg cacgcatccg gcaatgatat
    51001 ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag acttattaaa
    51061 gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg gcggtaacgc
    51121 gctttcttcg cagcaaattt cactacaaca acctttgtat ttttccgcac atactggaca
    51181 ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt cgcacggcgc
    51241 accgcactgg tggcaatgcg cgataatctc atcagatata cgttcgccca tccgctcgtc
    51301 aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg ccttacgggc
    51361 gtattcaata attccaccct cgatatgcca gactttattg aatccgttat gtttcatcca
    51421 ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt ttttatcttt
    51481 atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat ctgccggaat
    51541 ttccagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca tgtcgataaa
    51601 cagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat actcaccaac
    51661 attgctggca tcaaagtgag gatcgtcaat accgtcggca acgatacgat cgcggacctt
    51721 catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca ggcgtaagcc
    51781 atctaaagcc gagtcgaagg catagagctg cgcgcgaaat gttttaacat tgctcgcagg
    51841 tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa aaacattcag
    51901 cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga tgtggaaata
    51961 cttgtaaaac gaaatggtgg ttcgcggttc gctctcagcc aacattttgg cttttaacgc
    52021 gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg agatgggaaa
    52081 gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac attttacgct
    52141 acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc ccagtcttca
    52201 gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc ccgaataggt
    52261 tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg cttcatccgc
    52321 gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa ttgccctacc
    52381 cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt atgaaacgac
    52441 gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc gaacaagaac
    52501 gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg gaaactggca
    52561 tggcgtggtt ctggccggac cgccgaatcg ctcgctggac ggaagtgatc ggcatggaac
    52621 acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc cattttctga
    52681 cactggagct gggtgcgcgg cagtttggta tgcaggaacc ggggattggc gtttaccgcc
    52741 cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc tcaaataaat
    52801 cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa ggcgaagtgg
    52861 tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc cctttatttg
    52921 ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg tccggcgcat
    52981 gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa ttgattatgc
    53041 tgccgccaga gtgttctccg ccgctggatg atgccgaaac taccgccgcg tggatgaaca
    53101 aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta caccgtcgct
    53161 ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccatgcc ggatgcttca
    53221 gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa gctctctgga
    53281 ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc ttatgttcac
    53341 cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tgaaaatgac acccctataa
    53401 actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt gccgccttca
    53461 gtctggtaat gcccttctaa cccctctacg ttgagcagct tggcgttacc ggccactccg
    53521 ccctgaatat gtggtccggt attgtcttca gcattacatt tttattttcg gccatcgcct
    53581 caccgttttg gggtggactc gccgaccgta aaggccgaaa actcatgcta ttacgctctg
    53641 ctctcggcat gggcatcgtg atggtgttga tggggctggc acaaaatatc tggcagtttt
    53701 tgatcctgcg ggcgcttctt gggttacttg gcggatttgt ccccaacgct aatgctctta
    53761 tcgccacaca agtaccgcgt aataaaagcg gctgggcact gggtacgctc tccacaggcg
    53821 gcgttagcgg tgcgttgctc ggcccaatgg ctggcggcct gctcgccgat agctacggct
    53881 tacgtccggt attctttatt accgccagtg tgctcatact ctgctttttc gtcaccctgt
    53941 tttgcatcag agaaaaattc cagccggtca gcaaaaaaga gatgctgcac atgcgggaag
    54001 tggtgacatc acttaaaaac ccgaaactgg tactcagcct gtttgtcacc acgttaatca
    54061 tccaggtggc gacgggctca attgccccca ttctgacgct gtatgtccgc gaactggcgg
    54121 gtaacgtcag taacgtcgcc tttatcagtg gcatgatcgc ctcggtgcca ggcgtagctg
    54181 ctctgctgag tgcaccacga ctcggcaaac ttggcgatcg aatcggaccc gaaaagatcc
    54241 tgattacggc gctgatcttt tctgtactgc tgttgatccc aatgtcttac gttcagacgc
    54301 cattgcaact tgggatttta cgttttttgc tcggtgccgc cgatggtgca ctactccccg
    54361 ccgtacagac actgttggtt tacaactcga gcaaccaaat cgccggacgt atcttcagct
    54421 ataaccaatc gtttcgtgat attggcaacg ttaccggacc attgatggga gcagcgattt
    54481 cagcgaacta cggtttcaga gcggtatttc tcgtcaccgc tggcgtagtg ttattcaacg
    54541 cagtctattc atggaacagt ctacgtcgtc gtcgaatacc ccaggtatcg aactgatttt
    54601 tcgcctttca tacttgcaaa agcggagaat cagctatcct tttccctgaa acctcatcaa
    54661 ctcaaaggga gaatcgtgat gaccatgtac gcaacgcttg aagaagccat tgacgctgca
    54721 cgcgaagaat ttcttgcaga caaccccggc atcgacgccg aagatgcgaa tgtgcaacag
    54781 ttcaatgccc aaaaatacgt tttgcaggac ggcgacatca tgtggcaagt tgagtttttt
    54841 gccgacgaag gggaagaagg tgaatgttta cctatgctta gcggtgaagc cgcgcaaagt
    54901 gtttttgatg gcgactatga tgagatagag atccgccagg agtggcagga agagaataca
    54961 ttacatgaat gggacgaggg ggaatttcag cttgagccac cgctggatac cgaggaagga
    55021 cgcgcagcag ctgatgagtg ggatgaacgt taattactca tacgggccat gatgaatgtc
    55081 gatcggcagc agtaaagtat caaaaaccag ggagaatggc agatcaagga ttgtgacata
    55141 acgccaggcg gagtcacgca catcccattg cacaccggga taatattggt tgccatgccc
    55201 ctgccccggt atagtgcggc taatgatgct gccacagcca ctcagcaagg tcaccatgat
    55261 gctcaccaca attaaacgca tttgatacct cttccgtaaa aaaataccgg cataacgccg
    55321 gtatttttat tggctcgttg tttttgccag atgtggcgtg aacgccttat ccgacctaca
    55381 aaatcttgca aaatcaacac attgcaggag cgatgtaggc ctgataagca tagcgcatca
    55441 ggcaactacg tttttattgc gaagccgcat ccggtttacg caatgccagt ggattgagct
    55501 ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc cagacgcgga
    55561 aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga tcgcgattca
    55621 gcttctctgg cgtctcgttc agcgcctgtt caacatggcg gtcacgggcg atttccagca
    55681 ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta aatgacgggt
    55741 taaacactgc gtgcataaaa ccatcatcaa gggagcgttg acggttcatc tcaaggaacc
    55801 gatcggtatc aaccagtacc tgcggcggtg aatactcttc cgggatcagg aacagtttcc
    55861 agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca aacggtgaca
    55921 ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc aaccacgcca
    55981 tcccgacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg aacgcttcac
    56041 cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc cagccaagga
    56101 acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc agcacggaaa
    56161 acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccatat tctttcgttc
    56221 ctttacacca aataagcaaa atgctcaata gctttggcag gaacaacagc accatggtcg
    56281 aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg aacaactgcc
    56341 gtggttgcag gaagtattgc ggttcggtca acgcatgcac tacctgcaat gcagtagaga
    56401 gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc gtcaggaata
    56461 ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc atcaggttac
    56521 cgtggcacca gcggcggtca cgttttagct catcaagcaa gttaggcggt aattcttcat
    56581 aagaacccgg gagatcgtaa gcaatccaga ctccccaacc tgcacggcgc atcaacgccg
    56641 cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc ggcagcgggg
    56701 ccagtgcaca gtgctcgata aacggtttca cgcggataat cgcgttatgc ccccagtagt
    56761 gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca tacacgcggg
    56821 tcgcgaactg ctgacagcgt gcatacagcg tatccatacc ggacgctttc ggcgacgact
    56881 gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac caggccgcac aaacaatcac
    56941 cggtcattac cgagtcagca tccagcacca ccatatagct gtactggctg ccccagcgac
    57001 ggcagaagtc atcgatatta ccgcttttac gcttcacgcg acggcggcgg cggcgataga
    57061 aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt tgctctgcga
    57121 cgcagatatc cgggttgtaa ctgtcactca gaatgtagac atcaaagtgt ttggcattcc
    57181 cggtggcttt tactgattcc cacgttgcac gcaagccagc aaaaacacgg ttcacgtctt
    57241 cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt tcatcgccaa
    57301 ctgttgacgc agatatactg tatttatcgc gaccaataag tagttgcagg aagcccatta
    57361 acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg atcaggatac
    57421 cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc tgaccaacca
    57481 tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc atataccagg
    57541 tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg atggtaccga
    57601 cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc agatagcgcg
    57661 gtgttacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg gtacgccacg
    57721 ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttaagctga tcgcgccctt
    57781 cgtcatcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt tccagacgcg
    57841 cctttaccga gccttgcggg gagtcatcct cccgcgccca ggtgcggtgt tcggcatcca
    57901 gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg cttgcggcga
    57961 tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg gtagctccag
    58021 gtttcactca acgtctgatc ggcattcacc agcgcagcgc gcatttcagt ggttttcttg
    58081 gcatctttca ctttcacacg catcaccaga cgccagcctt tggtgaccgg gttatagcgc
    58141 accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt gaccggggta
    58201 tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc gatagtaccg
    58261 tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg cgtttgttgc
    58321 acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt gatggtgtat
    58381 ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta agcgacgatg
    58441 ttatcgttag tttcatcgtt ggttggaatt tccaccagct caacgctgcc tttaccccac
    58501 tcccctttcg gagtcaccca tgcgcttgga cggagatcgt aacgatcatc gagatcttca
    58561 aagcgggaga aatcacgacc gcgctgcaac agaccaaagc cttgcgggtt ttccatggag
    58621 aagctgctga ccgctaaatg tttcgggtta ttcaacggac gccagatcca ttcgccatta
    58681 ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt atttgctggc
    58741 gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc cagtttgccg
    58801 actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg ccctggcata
    58861 actacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata aatggttaaa
    58921 cgtttatcag ttggttttgg acgctcgatc cagaactctt tgaagcgtgg aaattcttca
    58981 cccgatggca aggcggtatc aattgccaga ccgcgggcag aaaggccata aacctgacct
    59041 gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc gtttttatct
    59101 ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt taccgtgtct
    59161 ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat tcgtttgact
    59221 gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc ctgatggtag
    59281 aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg attaaactgg
    59341 atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt gcttttgggc
    59401 gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc aatactgaaa
    59461 gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca acgcattttc
    59521 atcatttgta gtttatgttt cataattagt aagcacttcc ccctttgtgt gcttatatcg
    59581 atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg tatcgttcag
    59641 cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg tgcttctcct
    59701 aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa atttatttat caaccgtctt
    59761 acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa cttgaagcct
    59821 gcgcctggcg cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca taacccgaca
    59881 gggataagac gaaaaatccg agattagtta accatatatg aatccagtac ccgcgcaacg
    59941 tgaatatttc ctcgactcca tccgcgcctg gctgatgttg ttagggatcc cttttcatat
    60001 ttctttaatc tattcgagcc atacatggca tgtgaatagc gccgaaccgt cattgtggct
    60061 gacccttttt aatgacttca tccactcgtt ccgcatgcag gtatttttcg ttatatccgg
    60121 gtacttttcc tacatgcttt ttttacgcta tcccttgaaa aaatggtgga aagtacgtgt
    60181 cgaacgtgta gggatcccga tgttaacagc catcccccta ctgacattgc cgcaatttat
    60241 tatgctgcaa tacgtcaaag ggaaagcgga aagttggcct gggctgtcat tgtatgacaa
    60301 atataatacg ttggcctggg aattaatatc acatctgtgg tttttactgg tgttagtggt
    60361 catgacgacg ctgtgcgtat ggatatttaa acgcatcaga aataatttag aaaattctga
    60421 taaaacgaat aaaaaattct cgatggtaaa actatcggtg atttttttgt gcctcggcat
    60481 cggttatgcg gtaataagaa gaacgatttt tattgtgtat ccacccattc tgagtaatgg
    60541 cacgttcaat tttattgtca tgcaaacgct attttattta ccgttcttta tcctcggcgc
    60601 actggctttc attttccctc atcttaaagc cttgtttacc acgccgtctc gtggctgtac
    60661 cctcgctgca gcattggcgt ttgtcgctta tttactcaac cagcgctatg gcagtggcga
    60721 tgcctggatg tacgaaaccg agtcggtgat caccatggtc ctcggtctgt ggatggtgaa
    60781 tgtggtcttc tcctttggcc accgtttgct taacttccag tcagcgcggg tgacttattt
    60841 tgttaacgca tcgctgttta tctatctggt tcaccacccg ttaacgctgt ttttcggcgc
    60901 atacattaca ccgcacatca cctccaactg gcttggtttt ctctgtggcc tgatattcgt
    60961 agtagggatt gcgataattc tgtatgaaat tcatttgcgc atcccgttac tgaagttttt
    61021 gttctctggt aaaccggttg ttaagcgtga gaacgataaa gcaccagccc gttaagccac
    61081 atttacaata accattccac gggcaatatc gacgccagtc tgaccataac ccgcttccag
    61141 aaactggtag cgggttcttt tttgagaaca atctctttct ttgcatgacg atcaacccag
    61201 ttgatccgtc cccacctgtc cagacggagc tgccaggccg catcatactg gctctgaata
    61261 aagcgtttat caattaactg agccagcgtt tcgctctcta tcacgaagcc catttcagta
    61321 ttgagcaatg ttgaacgcgg atcgaaattg aaagaaccga taaacaccgt tttaccatcg
    61381 atgctaaagg ttttagcatg caggctggca ccggaattac cggttatgcc gcgatcgtgt
    61441 aacgtactac tttgttcacg cgtcggcttg agttcatata attccacgcc atagcggagc
    61501 aattttttgc gccagcgcgc gtatccggca tggacgacag caacatcgtt agcggcaaga
    61561 gaattggtta ggatcgcaat ctttacccct tttctcacca tccgtaagag ttgcgccaca
    61621 cctgcgcgtg tcggtacaaa ataggaagag ataatatcga tgcgttcact gggtgagccc
    61681 atgatatcga acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc ctcccctttc
    61741 gccggatcat cacttaataa acgtgttttc gcccagataa gcggcaacgt tccatcaacc
    61801 agatgattta taaacggact ggattccatt ttgcgtaagt aacgatgcgt catggcatcg
    61861 ttatgccagg aggcgggtaa ctcgatgcga tccgccattt caccttccgg gacatccagc
    61921 acctgctgta agggtgaaac cgatttgcaa taccagtagc gggcgaaatc atcggcaacg
    61981 tcctctacta cgggtcctat tgccatgaca tctaaatccg aaaaaagtgg ctcttcccct
    62041 gccccaaaat aggcatcacc aatatttcgc ccccctacca acgtcaccac gccatcgaca
    62101 gtgaaacttt tattgtgcat acggcgattg agacgggaaa agtcggtgat ataaccaagc
    62161 ggacgcagca agcgaaacga gaaaggatta aaaagccgga cttcaatgcg gggatggctg
    62221 tcaagcaagc gtaaaatgtc gtcaagtccg ggcgtattgt tgtcgtccag caacaaacgg
    62281 acgcgaacgc cacgctttgc tgcggctaac agggcggaaa acagtaaccg acccgacata
    62341 tcgtcctgcc agatgtaata ctgaacatcc agcgtatgct ctgacatttc ggccagacga
    62401 taccgggcgg caaacgcatc cagacttttc tccagaggga aaagaccaca ctgaccggga
    62461 tgttgcgaac acagtggcag caccgcgctc gccagccggg gcaaatcatt catctccttg
    62521 ttgggtaagg agtctttcgt agaggtgggc gttttcttca tcataacaga caaagtatac
    62581 ctgttcgggt aaagcgtgac gggtaataaa ttctgaaacg gtttttactg cgatttcagc
    62641 cgctgccgca cgagggtaac cataaacccc agtactgatt gcaggaaaag ccactgacgt
    62701 atagctgttt gccgccacca gtcgtaggct attgagatag gcatcctgca aaagctggtc
    62761 ttcgttttgt tcaccaccac gccagactgg cccgacggtg tgcactacgg ctttagcagg
    62821 aagatcgcct gcaagcgtaa taacggcatg ccccgtaggg caatcgccct gctgttgcct
    62881 gacttttaaa caagcatcca gcagggccgg acccgctgcg cgatgaatgg ccccatcgac
    62941 gccgccacct cccattaatg aaggattagc cgcattcaca atcacatcaa cggccagttt
    63001 ggtaatatca ccctgcacaa catgaatacg cgttttcata acgcctcctt acaccttctt
    63061 gtttcttaag tgtacagcag gctttttggt taaatcgcat ttttctgctc agcaacgtga
    63121 gttgttcaga aacttagtta tattcgatgt tcattacagc cagctttttc tgtttatcaa
    63181 gatactgcac ttttactgag gctatttgcg cgtttttgac attgccgctc attaacgctt
    63241 tgctggagta ggttttaccc ggaacagaac cattctgtgg gcagttcatt tctacgggtg
    63301 atgactgggt gctgacgtca catgccggtt ccacaatttg gccataaaaa tggatcacgc
    63361 ccccttgcgc ggtgcttcct gcgatggcaa taaaaggaaa aaacaaactg ccgagcataa
    63421 gagagtttaa aaaaggtctg aacatttatt ctatttcctc aatgattagt catccttgag
    63481 ggttgtgtta tccatacttt caggttggct gattcgcagc agaccattct ctccagattc
    63541 atcttatgct cgatatttca acaaattaag acttttctga agagggcggc cattgttgtg
    63601 ataaatgaag tgactgtcca tcagaaacag taacaactat tttcacccga tcgtccgggg
    63661 aaatatttaa actcaacttc gtcaaagcaa tgggttgatt agcaggcaat gaaagggtct
    63721 tttcttgctt cgtctgactt tgccctgaac tgccttcgcg cagggacaat atttgtactc
    63781 tgcacagaca agattgagta agagtgactt caggaataat ggtgtacata tccccttgct
    63841 gggtcgtatt aaaggttatc tgactggaaa gtgccgcaag gagtaataac gcattcatat
    63901 tcttctcccg aaaaaaaaca gggcttgcgc cctgtttctt taatacagat gatgtattag
    63961 tactgatgag cggtcgcgtt gttaccaaag ccaacctgag ttacgttgac ggtggaatta
    64021 gatgcagtct ggtcaaccgc tgcaccgttg ccgccaccga attgtttaac tgtcatatga
    64081 gagtctttac cgttccactg atcaagagtg gcgctgttac caaagccacg ttgggtcaga
    64141 tcgattgagc tgtcatctga gccctgacca acatctgcac cgttaccacc accatgctgg
    64201 gtaatagtaa gatcagagtt acgagcatca gcttgcagag caagtgcaga gttaccacca
    64261 ccgtactgat aaatattcag ctctgaattc gggccgctgt tattaccgcc accaccgtgg
    64321 ttaccgccac cgccgccgta ctgaggaaca acacctgcca gagcgctacc ggagaatacg
    64381 attgctgcaa ttgctgctac ttttaaaagt ttcatgtaaa acccccatcg gattgattta
    64441 aaagtcgaat ggaaattaac gttgtgtcac gcgaatagcc atttgcgact gtctctgcac
    64501 tacaattgcc gttttttgag taccatactg tgtaatattt gctttattac cagaaccttt
    64561 ctggataatc atcgcagtat taccataagc accttgcgaa atactggcat cattggcact
    64621 gcccgcctga tcaatatatg caaggttata atctcctgtc tggtcaatct ttgcccggtt
    64681 gctactacct tcttgcgcaa caaccgccaa aagttttgag cctccctgcc gtaactgagc
    64741 actattatta gtcccagctt gaccaattat ggctgcctga ttaaatgaag acttactcaa
    64801 ttcatttacc gcgaagttat attctgaatt agctaaatca taacctgctg cggctgcaat
    64861 cccaggcgca cccagtattg ttaacatcat aaataacaat ttgtttttca tgttgtcacc
    64921 ctggacctgg tcgtacattt aagaaattaa atcatttcaa cttggttgtt aacgcaacct
    64981 atattttgtt aacgctgtgt tacgatggaa agtatgtct
//
LOCUS       DABSYT020000016        63352 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.016, whole genome shotgun sequence.
ACCESSION   DABSYT020000016 DABSYT020000000
VERSION     DABSYT020000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 63352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.016"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            67..660
                     /locus_tag="ID725_RS06920"
                     /old_locus_tag="pgaptmp_001384"
     CDS             67..660
                     /locus_tag="ID725_RS06920"
                     /old_locus_tag="pgaptmp_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGV7850021.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTTSEWEQVGRLLAAGES
                     RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK"
     gene            720..1910
                     /locus_tag="ID725_RS06925"
                     /old_locus_tag="pgaptmp_001385"
     CDS             720..1910
                     /locus_tag="ID725_RS06925"
                     /old_locus_tag="pgaptmp_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HGV7850022.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVSSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVNGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESSDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            1923..2441
                     /locus_tag="ID725_RS06930"
                     /old_locus_tag="pgaptmp_001386"
     CDS             1923..2441
                     /locus_tag="ID725_RS06930"
                     /old_locus_tag="pgaptmp_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HGV7850023.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            2498..2773
                     /locus_tag="ID725_RS06935"
                     /old_locus_tag="pgaptmp_001387"
     CDS             2498..2773
                     /locus_tag="ID725_RS06935"
                     /old_locus_tag="pgaptmp_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGV7850024.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            2770..2925
                     /locus_tag="ID725_RS06940"
                     /old_locus_tag="pgaptmp_001388"
     CDS             2770..2925
                     /locus_tag="ID725_RS06940"
                     /old_locus_tag="pgaptmp_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGV7850025.1"
                     /translation="MTFPKNLSVDDLMADVAVVFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            2918..5365
                     /locus_tag="ID725_RS06945"
                     /old_locus_tag="pgaptmp_001389"
     CDS             2918..5365
                     /locus_tag="ID725_RS06945"
                     /old_locus_tag="pgaptmp_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGV7850026.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDELGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIQKNKSLASTIGLIVGGALALIGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPLFDWLGEKLQAAWQWFKKLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            5380..5859
                     /locus_tag="ID725_RS06950"
                     /old_locus_tag="pgaptmp_001390"
     CDS             5380..5859
                     /locus_tag="ID725_RS06950"
                     /old_locus_tag="pgaptmp_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7850027.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDEPLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            5859..7022
                     /locus_tag="ID725_RS06955"
                     /old_locus_tag="pgaptmp_001391"
     CDS             5859..7022
                     /locus_tag="ID725_RS06955"
                     /old_locus_tag="pgaptmp_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HGV7850028.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPIRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREDSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSIILATGRADIYT
                     ETPVRVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            7104..7322
                     /gene="ogrK"
                     /locus_tag="ID725_RS06960"
                     /old_locus_tag="pgaptmp_001392"
     CDS             7104..7322
                     /gene="ogrK"
                     /locus_tag="ID725_RS06960"
                     /old_locus_tag="pgaptmp_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HGV7850029.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(7596..8957)
                     /gene="yegQ"
                     /locus_tag="ID725_RS06965"
                     /old_locus_tag="pgaptmp_001393"
     CDS             complement(7596..8957)
                     /gene="yegQ"
                     /locus_tag="ID725_RS06965"
                     /old_locus_tag="pgaptmp_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGV7850030.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(9105..9437)
                     /gene="yegP"
                     /locus_tag="ID725_RS06970"
                     /old_locus_tag="pgaptmp_001394"
     CDS             complement(9105..9437)
                     /gene="yegP"
                     /locus_tag="ID725_RS06970"
                     /old_locus_tag="pgaptmp_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGV7850031.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(9628..10350)
                     /gene="baeR"
                     /locus_tag="ID725_RS06975"
                     /old_locus_tag="pgaptmp_001395"
     CDS             complement(9628..10350)
                     /gene="baeR"
                     /locus_tag="ID725_RS06975"
                     /old_locus_tag="pgaptmp_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGV7850032.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(10347..11750)
                     /gene="baeS"
                     /locus_tag="ID725_RS06980"
                     /old_locus_tag="pgaptmp_001396"
     CDS             complement(10347..11750)
                     /gene="baeS"
                     /locus_tag="ID725_RS06980"
                     /old_locus_tag="pgaptmp_001396"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGV7850033.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(11747..13162)
                     /gene="mdtD"
                     /locus_tag="ID725_RS06985"
                     /old_locus_tag="pgaptmp_001397"
     CDS             complement(11747..13162)
                     /gene="mdtD"
                     /locus_tag="ID725_RS06985"
                     /old_locus_tag="pgaptmp_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850034.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(13163..16240)
                     /gene="mdtC"
                     /locus_tag="ID725_RS06990"
                     /old_locus_tag="pgaptmp_001398"
     CDS             complement(13163..16240)
                     /gene="mdtC"
                     /locus_tag="ID725_RS06990"
                     /old_locus_tag="pgaptmp_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGV7850035.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            complement(16241..19363)
                     /gene="mdtB"
                     /locus_tag="ID725_RS06995"
                     /old_locus_tag="pgaptmp_001399"
     CDS             complement(16241..19363)
                     /gene="mdtB"
                     /locus_tag="ID725_RS06995"
                     /old_locus_tag="pgaptmp_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGV7850036.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAITLVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(19363..20610)
                     /gene="mdtA"
                     /locus_tag="ID725_RS07000"
                     /old_locus_tag="pgaptmp_001400"
     CDS             complement(19363..20610)
                     /gene="mdtA"
                     /locus_tag="ID725_RS07000"
                     /old_locus_tag="pgaptmp_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGV7850037.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            20890..20946
                     /locus_tag="ID725_RS07005"
                     /old_locus_tag="pgaptmp_001401"
     CDS             20890..20946
                     /locus_tag="ID725_RS07005"
                     /old_locus_tag="pgaptmp_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGV7850038.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            21218..21274
                     /locus_tag="ID725_RS07010"
                     /old_locus_tag="pgaptmp_001402"
     CDS             21218..21274
                     /locus_tag="ID725_RS07010"
                     /old_locus_tag="pgaptmp_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGV7850039.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            21546..21605
                     /gene="ibsA"
                     /locus_tag="ID725_RS07015"
                     /old_locus_tag="pgaptmp_001403"
     CDS             21546..21605
                     /gene="ibsA"
                     /locus_tag="ID725_RS07015"
                     /old_locus_tag="pgaptmp_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGV7850040.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            21826..22485
                     /gene="yegL"
                     /locus_tag="ID725_RS07020"
                     /old_locus_tag="pgaptmp_001404"
     CDS             21826..22485
                     /gene="yegL"
                     /locus_tag="ID725_RS07020"
                     /old_locus_tag="pgaptmp_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGV7850041.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            22482..23243
                     /gene="pphC"
                     /locus_tag="ID725_RS07025"
                     /old_locus_tag="pgaptmp_001405"
     CDS             22482..23243
                     /gene="pphC"
                     /locus_tag="ID725_RS07025"
                     /old_locus_tag="pgaptmp_001405"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGV7850042.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            23240..25180
                     /gene="yegI"
                     /locus_tag="ID725_RS07030"
                     /old_locus_tag="pgaptmp_001406"
     CDS             23240..25180
                     /gene="yegI"
                     /locus_tag="ID725_RS07030"
                     /old_locus_tag="pgaptmp_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGV7850043.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(25193..26545)
                     /gene="yegD"
                     /locus_tag="ID725_RS07035"
                     /old_locus_tag="pgaptmp_001407"
     CDS             complement(25193..26545)
                     /gene="yegD"
                     /locus_tag="ID725_RS07035"
                     /old_locus_tag="pgaptmp_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGV7850044.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            26678..27526
                     /gene="alkA"
                     /locus_tag="ID725_RS07040"
                     /old_locus_tag="pgaptmp_001408"
     CDS             26678..27526
                     /gene="alkA"
                     /locus_tag="ID725_RS07040"
                     /old_locus_tag="pgaptmp_001408"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGV7850045.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(27627..30945)
                     /gene="dgcE"
                     /locus_tag="ID725_RS07045"
                     /old_locus_tag="pgaptmp_001409"
                     /pseudo
     CDS             complement(27627..30945)
                     /gene="dgcE"
                     /locus_tag="ID725_RS07045"
                     /old_locus_tag="pgaptmp_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            31263..31904
                     /gene="udk"
                     /locus_tag="ID725_RS07050"
                     /old_locus_tag="pgaptmp_001410"
     CDS             31263..31904
                     /gene="udk"
                     /locus_tag="ID725_RS07050"
                     /old_locus_tag="pgaptmp_001410"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGV7850046.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            31996..32577
                     /gene="dcd"
                     /locus_tag="ID725_RS07055"
                     /old_locus_tag="pgaptmp_001411"
     CDS             31996..32577
                     /gene="dcd"
                     /locus_tag="ID725_RS07055"
                     /old_locus_tag="pgaptmp_001411"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGV7850047.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            32599..34452
                     /gene="asmA"
                     /locus_tag="ID725_RS07060"
                     /old_locus_tag="pgaptmp_001412"
     CDS             32599..34452
                     /gene="asmA"
                     /locus_tag="ID725_RS07060"
                     /old_locus_tag="pgaptmp_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGV7850048.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLAAQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(34505..34795)
                     /locus_tag="ID725_RS07065"
                     /old_locus_tag="pgaptmp_001413"
     CDS             complement(34505..34795)
                     /locus_tag="ID725_RS07065"
                     /old_locus_tag="pgaptmp_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7850049.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(34785..35123)
                     /locus_tag="ID725_RS07070"
                     /old_locus_tag="pgaptmp_001414"
     CDS             complement(34785..35123)
                     /locus_tag="ID725_RS07070"
                     /old_locus_tag="pgaptmp_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGV7850050.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            complement(35198..36781)
                     /gene="yegH"
                     /locus_tag="ID725_RS07075"
                     /old_locus_tag="pgaptmp_001415"
     CDS             complement(35198..36781)
                     /gene="yegH"
                     /locus_tag="ID725_RS07075"
                     /old_locus_tag="pgaptmp_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGV7850051.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(36982..37131)
                     /locus_tag="ID725_RS07080"
                     /old_locus_tag="pgaptmp_001416"
     CDS             complement(36982..37131)
                     /locus_tag="ID725_RS07080"
                     /old_locus_tag="pgaptmp_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850052.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            37440..38579
                     /gene="wza"
                     /locus_tag="ID725_RS07085"
                     /old_locus_tag="pgaptmp_001417"
     CDS             37440..38579
                     /gene="wza"
                     /locus_tag="ID725_RS07085"
                     /old_locus_tag="pgaptmp_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGV7850053.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            38585..39028
                     /gene="wzb"
                     /locus_tag="ID725_RS07090"
                     /old_locus_tag="pgaptmp_001418"
     CDS             38585..39028
                     /gene="wzb"
                     /locus_tag="ID725_RS07090"
                     /old_locus_tag="pgaptmp_001418"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGV7850054.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            39031..41193
                     /gene="wzc"
                     /locus_tag="ID725_RS07095"
                     /old_locus_tag="pgaptmp_001419"
     CDS             39031..41193
                     /gene="wzc"
                     /locus_tag="ID725_RS07095"
                     /old_locus_tag="pgaptmp_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGV7850055.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            41286..42125
                     /gene="wcaA"
                     /locus_tag="ID725_RS07100"
                     /old_locus_tag="pgaptmp_001420"
     CDS             41286..42125
                     /gene="wcaA"
                     /locus_tag="ID725_RS07100"
                     /old_locus_tag="pgaptmp_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGV7850056.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACTVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            42128..42616
                     /gene="wcaB"
                     /locus_tag="ID725_RS07105"
                     /old_locus_tag="pgaptmp_001421"
     CDS             42128..42616
                     /gene="wcaB"
                     /locus_tag="ID725_RS07105"
                     /old_locus_tag="pgaptmp_001421"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGV7850057.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            42613..43830
                     /gene="wcaC"
                     /locus_tag="ID725_RS07110"
                     /old_locus_tag="pgaptmp_001422"
     CDS             42613..43830
                     /gene="wcaC"
                     /locus_tag="ID725_RS07110"
                     /old_locus_tag="pgaptmp_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGV7850058.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKGVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            43805..45022
                     /gene="wcaD"
                     /locus_tag="ID725_RS07115"
                     /old_locus_tag="pgaptmp_001423"
     CDS             43805..45022
                     /gene="wcaD"
                     /locus_tag="ID725_RS07115"
                     /old_locus_tag="pgaptmp_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGV7850059.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            45033..45779
                     /gene="wcaE"
                     /locus_tag="ID725_RS07120"
                     /old_locus_tag="pgaptmp_001424"
     CDS             45033..45779
                     /gene="wcaE"
                     /locus_tag="ID725_RS07120"
                     /old_locus_tag="pgaptmp_001424"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGV7850060.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWVELSWHLRQRTTSKTKALYNKS"
     gene            45795..46343
                     /gene="wcaF"
                     /locus_tag="ID725_RS07125"
                     /old_locus_tag="pgaptmp_001425"
     CDS             45795..46343
                     /gene="wcaF"
                     /locus_tag="ID725_RS07125"
                     /old_locus_tag="pgaptmp_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGV7850061.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            46372..47493
                     /gene="gmd"
                     /locus_tag="ID725_RS07130"
                     /old_locus_tag="pgaptmp_001426"
     CDS             46372..47493
                     /gene="gmd"
                     /locus_tag="ID725_RS07130"
                     /old_locus_tag="pgaptmp_001426"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGV7850062.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            47496..48461
                     /gene="fcl"
                     /locus_tag="ID725_RS07135"
                     /old_locus_tag="pgaptmp_001427"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             47496..48461
                     /gene="fcl"
                     /locus_tag="ID725_RS07135"
                     /old_locus_tag="pgaptmp_001427"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGV7850063.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            48464..48943
                     /gene="gmm"
                     /locus_tag="ID725_RS07140"
                     /old_locus_tag="pgaptmp_001428"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             48464..48943
                     /gene="gmm"
                     /locus_tag="ID725_RS07140"
                     /old_locus_tag="pgaptmp_001428"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGV7850064.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            48940..50163
                     /gene="wcaI"
                     /locus_tag="ID725_RS07145"
                     /old_locus_tag="pgaptmp_001429"
     CDS             48940..50163
                     /gene="wcaI"
                     /locus_tag="ID725_RS07145"
                     /old_locus_tag="pgaptmp_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGV7850065.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            50166..51602
                     /gene="cpsB"
                     /locus_tag="ID725_RS07150"
                     /old_locus_tag="pgaptmp_001430"
     CDS             50166..51602
                     /gene="cpsB"
                     /locus_tag="ID725_RS07150"
                     /old_locus_tag="pgaptmp_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGV7850066.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            51795..53165
                     /gene="cpsG"
                     /locus_tag="ID725_RS07155"
                     /old_locus_tag="pgaptmp_001431"
                     /gene_synonym="manB"
     CDS             51795..53165
                     /gene="cpsG"
                     /locus_tag="ID725_RS07155"
                     /old_locus_tag="pgaptmp_001431"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGV7850067.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            53220..54614
                     /gene="wcaJ"
                     /locus_tag="ID725_RS07160"
                     /old_locus_tag="pgaptmp_001432"
     CDS             53220..54614
                     /gene="wcaJ"
                     /locus_tag="ID725_RS07160"
                     /old_locus_tag="pgaptmp_001432"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGV7850068.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            54616..56094
                     /gene="wzxC"
                     /locus_tag="ID725_RS07165"
                     /old_locus_tag="pgaptmp_001433"
     CDS             54616..56094
                     /gene="wzxC"
                     /locus_tag="ID725_RS07165"
                     /old_locus_tag="pgaptmp_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGV7850069.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQIAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            56274..57554
                     /gene="wcaK"
                     /locus_tag="ID725_RS07170"
                     /old_locus_tag="pgaptmp_001434"
     CDS             56274..57554
                     /gene="wcaK"
                     /locus_tag="ID725_RS07170"
                     /old_locus_tag="pgaptmp_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGV7850070.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            57551..58771
                     /gene="wcaL"
                     /locus_tag="ID725_RS07175"
                     /old_locus_tag="pgaptmp_001435"
     CDS             57551..58771
                     /gene="wcaL"
                     /locus_tag="ID725_RS07175"
                     /old_locus_tag="pgaptmp_001435"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGV7850071.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLSHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            58782..60176
                     /gene="wcaM"
                     /locus_tag="ID725_RS07180"
                     /old_locus_tag="pgaptmp_001436"
     CDS             58782..60176
                     /gene="wcaM"
                     /locus_tag="ID725_RS07180"
                     /old_locus_tag="pgaptmp_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGV7850072.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARAFPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            60334..61329
                     /locus_tag="ID725_RS07185"
                     /old_locus_tag="pgaptmp_001437"
     CDS             60334..61329
                     /locus_tag="ID725_RS07185"
                     /old_locus_tag="pgaptmp_001437"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HGV7850073.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            61572..62465
                     /gene="galF"
                     /locus_tag="ID725_RS07190"
                     /old_locus_tag="pgaptmp_001438"
     CDS             61572..62465
                     /gene="galF"
                     /locus_tag="ID725_RS07190"
                     /old_locus_tag="pgaptmp_001438"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGV7850074.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            62784..>63352
                     /locus_tag="ID725_RS07195"
                     /old_locus_tag="pgaptmp_001439"
     CDS             62784..>63352
                     /locus_tag="ID725_RS07195"
                     /old_locus_tag="pgaptmp_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGV7850075.1"
                     /translation="MNKETVSIIMPVYNGAKTIISSVESIIHQSYQDFVLYIIDDCST
                     DDTFSLINSRYKNNQKIRILRNKTNLGVAESRNYGIEMATGKYISFCDADDLWHEKKL
                     ERQIEVLNNECVDVVCSNYYVIDNKRNIVGEVNAPHVINYRKMLMKNYIGNLTGIYNA
                     NKLGKFYQKKIGHEDYLMWLEIINKTNGA"
BASE COUNT        15483 a        16177 c        17098 g        14594 t
ORIGIN      
        1 gtttatgaaa atgcagaaat aacgggtaac tggcatcatc tccggttttt attcagggag
       61 atgctcatgc ttattggcta tgtacgcgtg tcaacaaatg accagaacac ggaattgcag
      121 cgtaatgcgc tggagtgtgc aggatgtgag ctgatttttg aggataaaat cagcggcacg
      181 aagtccgacc gaccgggact gaaaaaactg ctcagaacat tatcagcagg tgacactctg
      241 gtagtctgga agctggatag gctggggcgt agtatgcggc atctggtcat tctggttgag
      301 gaactgcgcg aacgcggcgt taattttcgc agcctgacgg atgctattga taccagcacg
      361 ccgatggggc gctttttctt tcatgtgatg ggggccctgg cagaaatgga gcgagaactc
      421 attgtcgagc ggacacgcgc cggactggaa gcggccagag ccaaaggtcg tattggtggc
      481 agacgaccga aactcaccac gagtgagtgg gagcaggtcg gacggttgct ggctgcgggg
      541 gaatctcgtc aacgtgtggc gctgattttt gatattggcc tgtccacgct ctataaaaaa
      601 ttcccctcat cagcgacaaa gaataaattg tgtcatccct tagccaaccg ggacaaatag
      661 cctgacatct ccggcacaac tgaaaatatc actcacccat taaccacgga gttaaacgga
      721 tgagtgatta tcatcacggc gtgcaggtgc tggagattaa cgacggcacc cgcgtcattt
      781 ccaccgtatc caccgccatt gtcggcatgg tctgcacggc cagcgatgcg gatgcggaaa
      841 ccttccccct caataaacct gtgctgatta ccaatgtgca gagcgcaatt gcaaaggccg
      901 gtaaaaaagg cacgctggcg gcatcgttgc aggccatcgc tgaccagtca aaaccggtta
      961 ccgttgtcgt gcgtgttgaa gacggcaccg gcgacgacga agaaacgaaa ctcgcgcaga
     1021 ccgtttccaa tatcatcggc accactgacg aaaacggtca gtataccgga ctgaaagccc
     1081 tgctggcggc agagtcggta accggtgtta aaccgcgtat tctcggcgtg ccgggactgg
     1141 acaccaaaga ggtggctgtt gcactggcat cagtctgtca gaagctgcgc gctttcgggt
     1201 atatcagcgc atggggctgt aaaaccattt ccgaggtgaa agcctaccgc cagaatttca
     1261 gccagcgtga gctgatggtc atctggccgg atttcctcgc atgggatacg gtcagcagca
     1321 ccaccgccac cgcgtatgcc accgcccgtg cgctgggtct gcgcgctaaa atcgaccagg
     1381 agcagggctg gcataaaacg ctgtccaacg tcggggtaaa cggtgttaac ggcatcagcg
     1441 catctgtatt ctgggatttg caggagtccg gcaccgatgc tgacctgctt aacgagtcag
     1501 gcgtcactac gctgattcgc cgcgacggtt tccgcttctg gggtaaccgt acctgctctg
     1561 atgacccgct gttcctcttt gaaaactaca cccgcaccgc gcaggtgctg gccgacacga
     1621 tggctgaggc gcacatgtgg gcggtggaca agcccatcac cgcaacgctg attcgcgaca
     1681 tcgttgacgg catcaatgcc aaattccgtg agctgaaaac aaacggctat atcgtggatg
     1741 cgacctgctg gttcagcgaa gaatccagcg atgcggaaac cctcaaggcc ggaaaactgt
     1801 atatcgacta cgactataca ccggtacctc ctcttgaaaa cctgaccctg cgccagcgta
     1861 tcaccgataa atacctggca aatctggtca cctcggttaa cagcaattaa ggagcctgac
     1921 cgatggcaat gccgcgcaaa ctcaagttaa tgaacgtctt tctgaacggc tacagctatc
     1981 agggcgtcgc gaagtccgtc acgctgccaa aactgacccg taagctcgaa aactatcgcg
     2041 gtgcggggat gaatggcagc gcaccggtag acctcggcct tgatgacgat gcgctgtcaa
     2101 tggagtggtc gctcggtggc ttcccggatt cggttatctg ggagctttac gccgcaaccg
     2161 gtgtggatgc cgtaccgatt cgttttgcag gctcttacca gcgcgacgat accggcgaaa
     2221 cggtggccgt cgaggtggtc atgcgtggcc gtcagaaaga aatcgacacc ggcgagggta
     2281 aacagggaga agacaccgag tcgaaaatct ccgtggtctg cacctatttc cggctgacga
     2341 tggacggtaa ggagctggtc gaaatcgaca ccatcaacat gattgagaag gtgaacggcg
     2401 tcgaccggct ggagcaacac cgccgcaata tcggcctgtg attttcatcc ggtcagccag
     2461 gctgaccggt taaccctgat tcagaagtga gaaaaccatg aacaaagaaa atgtgattac
     2521 cctggacaat ccggtcaagc gtggtgagca ggttatcgaa caggtcacgc tgatgaaacc
     2581 caatgccggg acgctgcgcg gtgtcagtct ggctgcggtc gcgaactccg aagtcgatgc
     2641 actgattaaa gtgctgccgc gcatgacggc accgatgctg accgaacagg aggtcgccgc
     2701 gctggaactg cctgaccttg tggcgctggc cggtaaggtg gtcggttttt tgtcgccgaa
     2761 ctcggtgcag tgacgtttcc gaaaaatctc tcggtcgatg acctgatggc ggatgtggca
     2821 gtggtatttc actggccgcc atcagaactg tatcccatga gcctgaccga actcatcaca
     2881 tggcgcgaaa aggcgctcag gcgaagcgga aacacgaatg agtaacaatg taaaattaca
     2941 ggtattgctc agggctgttg accaggcatc ccgcccgttt aaatccatcc gcacagcgag
     3001 taagtcgctg tcgggggata tccgggaaac acaaaaatca ctgcgcgagc tgaacgggca
     3061 ggcatcccgt attgagggat tccgcaagac cagcgcacag ctcgccgtga ctggtcatgc
     3121 acttgaaaag gcacggcagg aggccgaagc ccttgccaca cagtttaaaa acaccgaacg
     3181 tccgacccgt gctcaggcga aagtgctgga atccgcaaag cgtgcggcgg aggacttaca
     3241 ggcgaaatat aaccgcctga cggattccgt taaacgccag cagcgggaac tggccgctgt
     3301 gggaattaat acccgcaatc ttgcacatga tgagctgggg ctgaaaaacc gtatcagtga
     3361 aaccaccgca cagcttaacc ggcagcgtga cgcgctggcg cgtgtcagtg cgcaacaggc
     3421 aaaacttaac gcagtaaaac agcgctatca ggcaggaaag gagctggccg gaaatatggc
     3481 ctcagtgggc gctgccggtg tggggattgc ggcggcggga acgatggccg gtgttaagct
     3541 gctgatgccc ggttatgaat ttgcgcagaa aaactcagaa ttacaggccg tgctcggtgt
     3601 ggcaaaagac tccgccgaaa tggccgcgct acgcaagcag gcgcgccagc tcggcgacaa
     3661 taccgccgcc tcggcggatg atgcggccgg tgcacagatt atcatcgcga aagcgggtgg
     3721 ggatgttgat gccattcagg cggcaacgcc ggtcacgctg aatatggcgc tggcgaaccg
     3781 tcgcacgatg gaagaaaacg ccgccctgct gatggggatg aaatctgcct ttcagctttc
     3841 aaacgataag gttgctcata tcggggatgt tctctccatg acgatgaaca aaaccgccgc
     3901 cgattttgac ggtatgagcg atgcgctgac ctatgccgca cctgtggcaa aaaatgccgg
     3961 tgtcagcatt gaagaaaccg ccgcaatggt cggggcgctg catgatgcaa aaatcacagg
     4021 ctcaatggcg gggacgggaa gccgtgccgt gttaagtcgc ctgcaggcac cgacgggaaa
     4081 agcatgggat gcactcaaag agcttggagt gaaaacctca gacagcaagg ggaatacccg
     4141 accaatattt accattctga aagaaatgca ggccagtttt gagaaaaacc ggctcggtac
     4201 tgcccagcag gctgaataca tgaaaaccat tttcggggag gaggccagtt cagccgccgc
     4261 cgtgctgatg actgccgcct caaccggaaa gctggacaaa ctgaccgctg cgtttaaagc
     4321 ctcagacggg aagaccgcag agctggtaaa tatcatgcag gacaacctcg gcggtgactt
     4381 taaggagttt cagtccgctt atgaggcggt ggggactgac ctgtttgacc aacaggaagg
     4441 cgcactgcgt aatctcacgc agacggccac aaagtatgtg ttaaaactcg acggctggat
     4501 ccagaaaaac aaatcactgg cgtcaaccat cggccttatt gtcggtggcg cactggcgct
     4561 tattggcatc atcggtgcaa ttggtcttgt agcctggccg gttatcaccg gcattaatgc
     4621 catcatcgcg gcagcaggcg caatgggggc aatcttcacg acggttggca gtgctgttat
     4681 gacggccatc ggggcgatta gctggccggt tgtggccgtg gtggctgcaa ttgtcgccgg
     4741 ggcgttgctt atccgtaaat actgggagcc tgtcagcgca ttctttggcg gtgtggttga
     4801 agggctgaaa gctgcatttg cgccggtggg ggaactgttc acgccactta agccgctgtt
     4861 tgactggctg ggtgaaaagt tacaggccgc gtggcagtgg tttaaaaaac tgattgcccc
     4921 ggtcaaagcc acccaggaca ccctgaaccg ttgccgtgac acgggcgtca tgttcgggca
     4981 ggcactggct gacgcgttga tgctgccgct taatgcgttc aacaaactgc gcagtggtat
     5041 tgactgggta ctggaaaaac tcggtgttat caacaaagag tcagacacac ttgaccagac
     5101 cgccgccaga actcatgccg ccacgtatgg caccggtggt tatattccgg cgaccagctc
     5161 ttatgcaggc tatcaggctt atcagccggt cacggcaccg gctggccgct cttatgtgga
     5221 ccagagtaaa aacgaatatc acatcagcct gacgggtggt actgcgccgg ggacacagct
     5281 tgaccgccag ttacaggatg cgctcgaaaa atacgagcgg gataaacgtg cgcgcgcccg
     5341 tgccagcatg atgcatgacg gttaaggagg tgacgaaaaa tgatgctcgc gttaggtatg
     5401 tttgttttta tgcgccagac gctgccacac cagaccatgc agcgtgaatc agattatcgc
     5461 tggccgtcaa attcccgtat cggtaaacgg gatgcctacc agtttctcgg cgttggcgag
     5521 gaaaacatga cgctggccgg tgtgctttat cccgaactga ccggcggcaa gctgacgatg
     5581 accacgctca ggctgatggc agaggaaggt cgggcgtggc cgttgctgga tggcaccggc
     5641 atgatttacg gcatgtatgt catcagcagg gtgagtgaaa cagggagtat tttctttgca
     5701 gacggcacac cccgaaaaat tgattttacg ctgtcgctca cccgcgttga cgaaccactg
     5761 gccgcgcttt atggcgatat cggtaaacag gcggaatcgc tcatcggtaa ggctggcagt
     5821 atggcgacta aattcacagg tatgacgggg gcgggataat gctggatgca ctgacatttg
     5881 atgcaggcag tacgctgacg ccggattaca tgctgatgct cgacagcagg gatattaccg
     5941 gcaatatcag cgaccgtctg atgagcatga ctctgacgga taaccggggc tttgaggctg
     6001 accagcttga tattgaactg aacgatgccg acgggcaggt cgggctgccg attcgtggcg
     6061 ctgtcctgac ggtgtatatc ggctggaaag gttttgccct ggtatgcaaa gggaaattta
     6121 ccgttgatga ggttgaacac cggggcgcgc cggatgtggt caccatccgc gcccggagtg
     6181 cagattttcg cgggacgctc aattcccgcc gtgaagactc ctggcatgac accacgctcg
     6241 gtgcgattgt tgaggcaata gcctcccgta acaggctgga agccagtgtc gcgccgtcac
     6301 tggccggaat taaaatcccg cacatcgacc agtcgcagga gtctgatgca aaattcctga
     6361 cccgccttgc tgaacgcaac ggcggtgagg tctcggtaaa aatgggaaaa ctgttgtttc
     6421 tcaaagcggg gcagggggtg acggccagcg gtaaaaaaat cccgcaggtc accataaccc
     6481 gcagcgacgg cgaccgccat cattttgcga ttgctgaccg tggagcctat accggtgtaa
     6541 cggcaaagtg gttacacacc aaagacccga aaccgcaaaa gcagaaggta aaactgaaac
     6601 gcaaaaagaa agagaaacac ctgcgcgcac tggagcaccc gaaagcgaaa ccggtcaggc
     6661 agaagaaagc gccaaaagta ccggaagcgc gcgaaggtga atacatggcc ggtgaggctg
     6721 acaacgtttt tgccctgacc acggtatatg ccacgaaagc acaggccatg cgtgctgctc
     6781 aggcgaagtg ggacaaactg caacggggcg ttgcggagtt ctccatcatc ctggctaccg
     6841 gtcgtgcaga tatttacacg gaaacaccgg tcagagtgtc aggctttaag cgcgtcatag
     6901 acgagcagga ctggaccatc actaaggtga cacattttct gaataatagc ggcttcacga
     6961 cgtccttaga gcttgaggtc aggctttctg atgtggaata cgaaacagaa gatgatgaat
     7021 gatgttttta ttttatctgt ttgttttata aggataaatt aactaaaatg gcaccatcaa
     7081 caaaaccgga agaggtgctc gcgatgtttc attgtccttt atgccagcat gccgcacatg
     7141 cgcgtacaag tcgctatatc actgacacga caaaagagcg ttatcaccag tgtcagaacg
     7201 tgaattgcag cgccacgttc atcacttatg agtcggtaca gcgatacatc gtgaagccgg
     7261 gagaagtcca cgccgtaaga ccgcacccgt tgccgtcagg gcagcaaatt atgtggatgt
     7321 aattacaaac agaaagcccc tcagttgagg ggcttttttt gtcgatgtgg tcaatatgtg
     7381 gacgtgacca gaaataaatc cttttatttc attgtattac gcgtaaaaaa taagcccgtg
     7441 taagggagat tacacaggct aaggaggtgg ttcctggtac agctagcatt ttatgggtta
     7501 tgtttttcag cgaaacggat gataacctta ataaatgcag ctgtatgtga tcggtttcta
     7561 agaattttcc atccgggaaa aataatcgaa attaatcact taccgtgggg gttacgcgta
     7621 gtttccccgg agaaattacg catcagcagc gcgtaattta gctcaagatc ctgcggcacc
     7681 gggagccaca cagtataacc atcgcctggt gctaccggca tagcttcgcc tttggcgttt
     7741 tccatgtgct caagggtaaa gttaatgttg ccttgcggcg tcatcagctc aaggctgtcg
     7801 ccaacggaga atttattttt caccgctacc gccgcgaggt cccccttgcg ctcaccggta
     7861 aactcaccaa caaactgctg gcggtcagaa actgaataac cgtattcgta gttctgataa
     7921 tcgtcgtgag tatgacgacg caggaaacct tcggtatagc cacgatgcgc cagaccttcc
     7981 agagtttcca gcaggctggt atcgaacggt ttgcccgcag cggcatcatc gatagcttta
     8041 cgataaacct gtgcggtgcg tgcgcaatag tagaaagatt tagtacggcc ttcgattttc
     8101 agcgaatgca cgcccatttt ggtcaggcgt tctacatggg cgatggcgcg cagatctttc
     8161 gagttcatga tgtaagtgcc gtgctcatct tcaaacgcgg tcatatactc gcccggacgc
     8221 tgggcttctt cgatcataaa cactttgtcg gttggcgcgc cgatacccag cgtcggctca
     8281 acattttgca ccggaatcgg ctcgtacttg tgtacgatgt tgccaacgtc atcttctttc
     8341 ccttcctgga cgttgtactc ccagcggcag gcgttggtgc aggtaccctg gttcggatcg
     8401 cgcttgttga tatagccaga gagcaggcag cgaccggagt aggccatgca cagtgcgccg
     8461 tgaacgaaga tctcgatctc catatccggc acctgattgc ggatctcttc aatctcttcc
     8521 agcgacagct cgcgagagag gatcacgcgg gtcagtccca tttgctgcca gaatttcacc
     8581 gtcgcccagt tcacggcgtt agcctgtacc gagagatgga tcggcatttc agggaagtgc
     8641 tcacgcacca gcataatcag ccctggatcg gacataatca gcgcatccgg ccccatttcc
     8701 accaccggtt tcaggtcacg gataaaggtt ttcagcttgg cgttgtgcgg tgcaatgttg
     8761 accacgacat aaaacttttt ccccagcgcg tgggcttcat tgatgccgag ctgaaggttt
     8821 tcgtggttga attcgttgtt gcgcacacgc agggagtaac gcggctggcc cgcataaaca
     8881 gcatctgcgc cataagcgaa agcgtaacgc atatttttca gcgttcccgc cggggaaagg
     8941 agttccggtt taaacataat tctctcgttc tgatgacagg tcagatccgc ttcacctgat
     9001 gaagcggtta ggggagtgcc cccactttaa gggcgggcat tgtagcgctg taggggggat
     9061 ctggtaaagc gccggacgcc aacaacccac gcccggcatc gtaattacgt attgtctttc
     9121 accgtctggc ttgtgccatt ggctttaacg gaggcaattc cggtttcacg agattgcgcg
     9181 gtggcataca tctggctgga gccgataatt tgatgattgg cggctttcag attgaaatag
     9241 aatttgccgt tgcttgccgt ttttttctca tagcgttctt cttgcgggct gttgctacgc
     9301 accgacgcga tgcccttttc cgcagaggct tttgaggtat acagctcgct ggtgaggatc
     9361 gtctcaccat tgcccgcttt tagcacaaac cggaactgac tatcactgct cttactgagt
     9421 tcaaaccaac cagccataat gactccttga ttgaaattgc gtatcgaaaa cagcatctaa
     9481 aggtgctgtt taccgattaa agtatgagcg attttctgat gcctgccagg gcggagagat
     9541 aatgtcagtt tgctgaccat gcgtgggata atgttgccgg atctggtctg aatgccatct
     9601 ccggctaaca aagtaatgtc gctaaaacta aacgatgcgg caggcgtcgg cttcccagcg
     9661 gtaaccgacg ccgtaaacgg cgcggataaa tgactgttcg gcgtcgagag attccagctt
     9721 gcggcgcagg tttttaatgt ggctgtcgat ggtacggtcg gttactacgc ggtagtcgtc
     9781 ataaagatga ttgagcaatt gctcgcggga gaacactttt cctggttcat gagagagcgt
     9841 tttcagcaga cgaaattccg caggcgtcag gtcaagcatt ttaccgcgcc atgaagcctg
     9901 aaaacgacct tcgtcgataa tcaacgggct ttcagcatcc tgttgctgca actcacgctg
     9961 cggtttgcaa cggcgcaaaa tggttttgac acgcgctacc acttcccgtg ggctgtacgg
    10021 cttgcaaatg tagtcatctg cgccaatctc cagccctagc aggcggtcga tctcttcgat
    10081 ttttgctgtc accatcacga taggaatgtc agaaaaacga cgaatttccc ggcacagcgt
    10141 caggccatcg gtgccaggga gcatcagatc caacaggatc agatccggcg gcgtctggcg
    10201 cacgtacgac agtacctgat cgccgtggct gataagcgtc ggcgcatagc tcgcagcacg
    10261 cagataatca atgagcaact gtcccagctt cggttcatct tccacgatca aaatacgcgg
    10321 tgtgttttcg tcgattggta actcggtcat acttctctct gtaaatcccg ttccagcggt
    10381 aactctactg taatgcttac cccgccaaaa ggcgaatggg cggcaataat gcggccatta
    10441 tgtgcttcaa caatgttcag gcaaatcgcc agccccagcc cggaaccgcc gctggcgcgg
    10501 ttgcgggaac cttcggtgcg ataaaaacgt tcaaacaatt tttgtagctg atcgtcactg
    10561 acacctggcg cactgtcggc aaaggtcagg cgcaccgttt tgtcgtgctg ctcggcagag
    10621 attttcaggc tgccgccgct gtcagtgtag cgcaggctgt tttccagtaa gttattgaat
    10681 aactgcatta aacggtcgcg atcgccaaat acggtgatac tgtctggcag ggaaaattgc
    10741 agtttcaggc cacggctggc aaatcgttcg cgaaatgcgc cgcctgccac ttccagcagt
    10801 gggatcaaat ctaccggtgc tttttgatag gcgagagcgc cttcatcaga catcgacaac
    10861 tgatgaagat cgtcaaccag tttggtcagt gtaccgacct ccgcctgtaa agacgccacc
    10921 gtctccggcg tgaatttgcg cacgccatcc tgaatggctt ccagttcacc gcgcagcacc
    10981 gctaatggcg tacgcagttc gtgagaaata tcggccataa aatcgcgccg catttgctgg
    11041 tttttctcca gcgtgctggc gagctggttg aagtcttgcg ccagtttgcc cagttcatct
    11101 tcactggtgg gcgttacgcg ggtagtgaaa tcgcccgccg ccagtttgtg cgtgccatcg
    11161 acaagtcgtt ttaccggtgc cagcaaaccg cgcgccagca gaaaagtagc gagtgccgcg
    11221 agtaacgttg ccagggcgac aatcagccag ctggtttgcc gctgttgttt atcgaaattg
    11281 atatcagtat tgcgggtcag ccgctcaaca ggggaggcaa tcaccgcgcc cacttccgca
    11341 ccgttgacca gaatgggtcg ccgtgtaccg tcaggtggaa tcggcgctcg cggaccaacc
    11401 agcaccttgt tgttttgatc aaccacccag aactgagtac gccagccgtg tggtggcata
    11461 cccgggccgg gtttatcttc cgaattatcg tgttcaaatg aacgcaggat ctgaaagaca
    11521 aagcgatcat tgttgcgcag gaagcgccag ttgccatgct gcgcatactg ctcgccaagc
    11581 gcatcactta acagttgtaa tcgctgttca ttaccatgct tgatgtaatc aataaagcca
    11641 cgctcaaaac tgatacgcac cgcccagtgc atactgatca gcaagacaat gcaggtggcg
    11701 aaaatcgcca gaaacagttt gccggtaata ccaggtcgcc agaacttcat tgcgcgctcc
    11761 tttttcgccg cgaaatagct acattttgat gcgtatcgtt cggcactctg gcaaagatga
    11821 acgccggaag ggcgatgata aacgccatgc taagccaggt gtacataaag acggtttgtg
    11881 tggtgctgct gtcgacgctg acatgctgcg aaccaaaaag tcccagcaac agcccggcga
    11941 tggtgacgcc gatactcatc gacaattgca taatcatcga cagcagactg ttgccgctgc
    12001 tcgccagatt gtccgggaga tctttcagcg tcagggtgtt catggaggag aaacgggtcg
    12061 agttgaccat cccttgtaaa aacaggacga acggcaaaac gtagtaccag cccaacagag
    12121 cggtagtcat aaacaacagg gtgaccagcg acagacccag cgtggtcgct accagtaccc
    12181 gacgataacc aaagcgattc accacctgta caacaatccg cttcatcccc atgctgccaa
    12241 gcaccatcgg gatcatcatc agtccggcat gaaacggtga gaagccaagg ccaatttgca
    12301 ggaaaaccgg cgtcataaag ggcaacatgc cgctaccgat acgtccggca aagctccccg
    12361 ccaggcccag cgaaaaggta cgagtgcgga acagtttcag actgaaaaga gcgcggttgt
    12421 tatttctggc gtgcagcaga taaagcacca gtgccaccac accaactgcg accaggccag
    12481 taatcgccag cggcgataaa cctgtacctt tactgccgtc cagcgccagg gttaataccg
    12541 ccatgccaac cgccagcaat aaaaagccgg aaagatcaaa gcgccgcgtc tgcatggtgt
    12601 agttcggcat taacatcaat gtggcgatcg caccgataat ccccaccgga atgttgatca
    12661 aaaagatcca gtgccacgac gcgtactcca ccagcagacc gccgagcgcc ggaccgagca
    12721 gcggaccgac ctgaccgggt aacgtgacaa aggtcatcgc cgccatatat tgctcgcgcg
    12781 gtacgatttt catcaccgtc aacctgccga ccggcaccat catcgcaccg ccaacgccct
    12841 gtaacgcgcg tgccagcaac agttcgttca gcgtgccgga aagcgcgcaa aacagtgaac
    12901 cgagagtaaa cagcacgata gcggtaaaga aaatattgcg cacgccgact ttgtccgcca
    12961 gccagccgct ggcgggcagc atcaccgcca cggtcagcac ataagagaca atgaccatgt
    13021 gcatatgcaa cggactttcc ccgaggcttt gcgccattga gggaagggcg gtgtttacga
    13081 tggtggtgtc cagcgactgc ataaagaagc cgaaagccac aatccacaat tgccagcggg
    13141 tgctgtcggg aagatctgtc atttactcgg ttaccgtttg tttagattta cgcgaaaaac
    13201 gcagccgcag acggtcgaaa aagagatata ccaccggcgt ggtatacagc gtcaggagct
    13261 gactcatcac cagaccgccg acaatggtga tccccaaggg ttgccgcagc tccgagccgt
    13321 caccgcccga caacaccagc ggcagcgcgc caaacagcgc cgccagggta gtcatcataa
    13381 tcgggcgaaa acgcagcaga caggcctgga aaatagcttc ctgcggcgtc aggttaccgt
    13441 gccgttgggc ttcaagcgca aaatcgacca tcataatggc gtttttcttc acaatgccga
    13501 ttaatagcat gatccctatc agggcgatta ggctgaacgg ggcattgaac agctccagcg
    13561 ccagcagcgc tccaacgccc gccgagggca gggtggagag aatcgtcagc ggatgtacgt
    13621 aactctcgta aaggataccc agcacgatat acaccgtggc gatggcggcg ataatcagga
    13681 tcacctgtga gttcatcgtc tcctggaaca cctgcgccgt gccggcaaaa ctgccgcgca
    13741 ccgtcgaagg cacaccaagc tgggtcattg cgcgatcgat cgccgcactg gcgtccgaga
    13801 gcgattttcc ggtcggcagg ttgaacgaga tggtcgaggc tgccgataat ccctgatgat
    13861 tcaccgatag tggggcattc gccggttgcc atttagcgaa atacgacagc gggatcgctt
    13921 ttccttcgtt attgattacg aacatttttt ccagcgcgct gatgtcctgg gtatagcgcg
    13981 gatccacttc catcaccact ttatactggt tcatcggctg gtaaatggtc gagatttgcc
    14041 gctgaccgaa ggcgttattt aacaggctgt tggcggcctg tacgtcgatt cccagccgtg
    14101 ccatggtgtc gcgatcgtaa accagattca tctccgcgcc gttatcctgc tgatcggagt
    14161 tcacgtccgc cagttccggc aatgtcgcca gttttttgcg gattttcggc tcccattcgc
    14221 gcagtgccgc caggtcgtcg gataacaacg tgtactggta gctggcgttc gactgacgtc
    14281 cgccaacgcg aatatcctgt accgccatca ggaacagatt cgcccccggt tctttcgcca
    14341 gcttcacgcg tagacggtca ataatttgct gcgctgtttc gctgcgttcg tcgcgtggct
    14401 tgagggtgat aaacatcatc ccgctgttca ctcgcgaacc gccggtaaag ccggtgacat
    14461 tatccactgc cggatcgtca cggataattt tcatgaaatc ctgcaactta ccgcgcatcg
    14521 cctgaaacga aatactctga tccgcctgta tcccgcccat caacacgcca gtgtcctgct
    14581 ccgggaagaa ggttttcgga atcgagatat atagccagat attcaaagca atggtgccaa
    14641 gcagcaccat gcccaccaga cgggtatgat tgagcaccca tttcagtgac ttaccatagc
    14701 cttgttgcag ggctaccaac atgcgaccaa aaccacgcag tcgcttttgc tcgcgcggct
    14761 tgctggcttt cagcatccag ccacacatca ttggcgttaa tgtcagagaa accagcaacg
    14821 aaataccaat ggcgacagaa agcgtcacgg caaactcgcg taacagtcgg cccggcaatc
    14881 cgcccatcaa cagcagcggc aggaacaccg ccaccagcga cagactcatc gacagcaccg
    14941 taaaaccgac ttcgcgagta ccttgcagtg cggcttgcaa cggtttcatt cccgcttcca
    15001 gatgacgtgc aatgttttcc agcaccacga tggcgtcatc caccacgaaa ccagtagcga
    15061 tggtgagcgc cattaacgaa aggttattga gactgaatcc gcacaggtac atcgccgcaa
    15121 acgtaccaat cagcgaaacc ggcaccgcaa cggcgggaat aatagtggcg cgacccgagc
    15181 gcaggaataa aaagaccacc agaatcacca gcgccaccga gataatcagc gtttgctcga
    15241 cttcttccag cgaggcgcga atggtggggg agcgatcctg ggcaatttgc agatcaatcg
    15301 ccgccggaat ggtttcctgc aactccggta attttgcccg gatgctgtct accgtctgga
    15361 taatattggc ttccggcaat ttgcggatca tcagtaaaat agccggtttg gcgttggtca
    15421 tcccggcgtt gcgcacatcc tgcactgagt cggtcaccgt cgccacatcg cccagacgaa
    15481 ccgcgccgcc gttgttgtag tgaataatca gcggctgata ttcagcggcg gttttcagtt
    15541 catcgttggt ctggatctgc cagcggtgag tgccatcttc cagcgccccc tgcggtttac
    15601 gcacgttggc attgctgatg gcagtgcgta cgtcgtccag cgacacgccc tgattaaaca
    15661 gcgcctgcgg gttcagcccg acgcgcacgg cgggcagcga gctgccgccg acatcgacat
    15721 caccgacacc gtcgatttgc gaaatcgtcg gagccagctg cgtcgaggcg aaatcgtaca
    15781 gttcaccctg cgaataagta tcggaggtta gcgtgaggat cataattggc gcatccgacg
    15841 ggttcgcttt gcgataagtc gggcggctgg gcatcccact gggcagcaga ctttgtgcgg
    15901 cgttgatcgc cgcctgcaca tcacgcgccg caccgttgat atcccggtca aaatcaaact
    15961 gcaaaataat acgcgtgctg ccgagcgaac tgctggaggt catttcactg accccggcaa
    16021 tgcgcccaag tgagcgctcc agcggcgtgg caacggaaga cgccattgtc tccggcgacg
    16081 caccgggcag cgaggcgctg accataatca ccggaaaatc gacctgcggc agcggggcga
    16141 ccggcagcat acggaaaccc agtatgccgc acagggtaat ggcgaccgac agtaaaatcg
    16201 tcgccaccgg gcggtaaatg aagagggcaa aaaacttcac ttacgcctcc tcttcatggc
    16261 gagcaaagcg acttttggtc cacaatgcca ggcggtcaaa cagcagataa atcaccggcg
    16321 tggtgaacag cgtcagcacc tggctgacaa tcaggccgcc gaccatgccg atacctaacg
    16381 gacgacgtag ttccgcgccg accccggtac tcaacatcag cggcagcgcg ccaagcagag
    16441 ccgccagggt ggtcatcagg atcggacgaa aacgcaacag acaagcctgg tagattgcat
    16501 cgcgcggcga catgccttgc tcgcgctcag cagccagcgc gaagtcgatc atcatgatgg
    16561 cgttcttctt cacgataccg atcagcaaaa taatgccgat aatcgcaatc acatccagtt
    16621 cgctaccggc aatcattaac gccagcaggg cgccaacacc tgccgtgggt agcgtcgaga
    16681 gaatggtgat cgggtgaata aagctctcgt acagaatgcc gagcacgata tacatcgcca
    16741 ccaccgccgc gacaatcagc cagacagtgc tgcccagcgc cgactggaag gcgagggtgc
    16801 tgccctggaa ttgcgtggtg atatccaccg gcagattcag cgtcttctcg gtgtccataa
    16861 ttgcctgcac cgcatcgccc agcgaatagt tatccggcac gttaaaggag atggtcgtta
    16921 ccgggaactg atccagatgg ttgatggaaa gcggcgcaaa acgctgctca attttggcaa
    16981 ttgagcttag cggcaccacg ccgccgtcgc tgctggtcag gcgaatggta tccagcgccg
    17041 cgaggcctgg ggtattttcg gtattgtgct ccagcactac acgatactgg ttagcctgag
    17101 tataaatagt ggaaatcagt cgctgaccaa acgcgttgta cagggcgtta tcgacatccg
    17161 ccatgctgat ccccagacgg ctggcgctgt cgcgatcaac attgacatac gccaccagcc
    17221 ctttgtcctg ccagtcgctg gagacatcag aaatctgtgg cagctgctgg agtttttcca
    17281 tcaactgtgg cacccaggta ctgagcgcat ccagtgacgt ggcctgcaag gtaaactggt
    17341 actgggtgcg gctgacctga gtatcgatgg tcaggtcctg cgttggttgc aggaagagat
    17401 cgacgcccgg cactttatcc accgccgttt gcagacgggc gatgactttt tgcacccgat
    17461 catcacgttc atccaacggt ttgaggttga tctgcaaacg cgcactgttc agcgacgggt
    17521 tagtgccatc aacgccaaca aatgaggtca gactttgcac tgccggatcc tgcaaaatca
    17581 cgtccgcgac ctggcgttgc cgctgggcca tattggcaaa ggagctggat tgcggtgcct
    17641 gcaaagtgcc ctgaataatg ccgttatcct gtaccgggaa gaaacctttc ggaatgaaca
    17701 cccacaatag aacgctaagc agcagcgtgc tgagcgccac gcttaaggtc agccacggat
    17761 gattcagcac tttcgccagt ccacgaccat aggcggcgat tatcctgtcg aacatttttt
    17821 ccgaggcacg ggagaagcgg ttctgtttac gcaacgactc ctggctgagc atccgtgcgc
    17881 acatcatcgg tgtcagggtt agcgacacca cggctgagat taaaatcgct accgccaggg
    17941 taatagcaaa ttcgcggaac agtcgcccga cgatatcgcc cataaacagc agtgggatca
    18001 acaccgcaat cagcgagaag gtcagcgaga taatggtaaa gccgatttca cctgcgcctt
    18061 tgagcgccgc cgccaacggt ttttcgcctt tttcgatata gcgggaaata ttttcgatca
    18121 ccacgatggc gtcatcgacc acgaacccgg tggcgatagt taacgccatc agcgtcaggt
    18181 tattgattga aaaatcgaga aacaccatga ccgcgaaggt gccgattaac gacagcggca
    18241 ctgcaacgcc gggaatgatg gtcgccggaa tattgcgcaa aaacaggtag ataatcatga
    18301 ctaccagcgt gatagccatc atcaattcaa actgagtatc atcgacggat gcgcggatat
    18361 tggtggtacg gtcagaaagt accgtcacct tcaccgattt cggcagactc tcggtgagct
    18421 gtggcagcat ctgccggatg ctgtcggcgg tggagataat gttagcaccg ggctggcgct
    18481 gaacattcat cacaatggcc tgttctttat tcgcccatgc gccgagccag ctgttttctg
    18541 caccttgctc tacagttgcg acatcgccca gacgaatcgg cgcgccgttc tggtaggcga
    18601 tgattagctg gcgatactct tcggcggatt gcatctggtc gttagcggaa agcgtaaccg
    18661 cacgggaagg gccgtcaagg ctacctttag ccgagttaac gttagcgccg gtaatggcgg
    18721 tgcgcacggt ttcgctggtc aggccgaggg cggcaatcgc ctgagcgtta agtttgacgc
    18781 gaacagccgg acgctggccg ccggaaagcg tcaccaggcc gacgccggaa atctgcgaga
    18841 ttttctgcgc gacgcgggtt tccaccatat cttccacttg cgtcatcggc atggctgttg
    18901 aggtgacggc gagcgtcatg atcggcggat ctgccgggtt cactttgctg taaaccggcg
    18961 ggttaggcag atcgctcggc aacaagttgg tcgcagcgtt aatcgcggcc tgcacttcct
    19021 gctcggcgac atccagcggt aatgttagct ggaactgtaa agtgataact gacgcaccgc
    19081 cggaactttg cgacgacatc tgtttcaggc cagacatctg cccgaactgg cgttcgagcg
    19141 gcgcggtaac ggcagaggtc atgacatccg ggctcgcacc tgggtagagc gtgaccacct
    19201 gaatggtcgg atagtccact tccggcagcg ccgaaacggg cagggcgcga taaccgataa
    19261 tcccggcgag taagatcgcc accatcagca gcgtggtggc cacaggacgc ataataaaca
    19321 ggcgcgacgg gccgcctgtg ctgctcgggg gtaacacttg catcaggagc gagctccttt
    19381 tttcgcgtat tcgcggctgg tggctttctc ttccggagtg gtggcgctct gggcttccac
    19441 cacttccact ttcgcccctt cggtcaggcg atcaatgccg tctgtcacca cgcgatcgcc
    19501 cgcagaaata cctgcgcgga tcaccacttt ctgactgtcc tgaatgcccg gcgtcaccag
    19561 atgtttgctg accttgtttt cgctattcag cacccagaca aaatggcctt cattgcccat
    19621 ttgcagggcg gctgttggga tcactacggc gttttgttcg gtgtcgacta acatgcgcgc
    19681 gttaacaaac tgattgggaa acagcgcatc atcctgatta ttaaagcgtg ctttcacttt
    19741 aatcgtaccg gtagtggcat cgatttggtt atcgagactt aacagcgtgc cttcacttaa
    19801 tttcttcgag ttggtgcgat cccaggcttc taccaccagc ggttttccgg ctttttgcgc
    19861 ctgcacgacg gtagcgatat cgctttccgg cagggtaaag agcaaatcga taggatgcgt
    19921 ctgggtgatc accacgatcc cggtggtatc accactggag atttggttac caacatcaac
    19981 ctgcttgaga ccaacgcgac catcgactgg tgcggtaata cggctccagt cgagttgcag
    20041 ctgcgcgctg gcgacgcttg cttcatcagc cttaatggtg ccttcggttt cactgaccag
    20101 cgcctgttgg gcatccagct cctggcggga gacgagattg gtttttgcca gttgctgata
    20161 acgcgccaga tcacggcggg cgttggtaag cgtggcttta tcttttgcca gttggccctg
    20221 ggcttgtgct aatgcaactt tgaactggct ggggtcaatt tctgccagta aatcgccagc
    20281 tttgacctgc tggccttcct ggaaatgtaa cgccatcagt tgaccgtcca cgcggctacg
    20341 caccgtaaca gtattagcag cggtaatggt gccaagcccg gtgaggtaac gcggaactgc
    20401 ctgttctacg gcggtcgccg cctgaaccgg ggctaatggg ccggaacgca taccacggcg
    20461 accacccgct ggcgattgct gcgcttgttt cgtcgcccct ggggctgcac tctgggagtc
    20521 attgcggcct tgccagaacc agaatgcggc gatggcggcg ataactacca cgattacgat
    20581 tacccaacgg gatttataac tgcctttcat cgttaagagt ttctcttcct gaaacgtttc
    20641 gcggaatgat actagtgtag ccagccaata agggagaaaa atggaggaat tatgaaacac
    20701 tatcgggaat cgtctgaatg gcgggcacat ttgcgagcac gcatccagta ataacacagg
    20761 aaactatttt atctacgcgt tagcgataga ctgcttgcat ggcgaaagga ggtaagccga
    20821 cgatttcagc gggacgctga aacgggaaag ccccctcccg aggaaggggc cataaataag
    20881 gaaagggtca tgatgaagat actcatcatc gtggtgctct tagtgattag ctacccagca
    20941 tactaagact accagggcgg gggaaacccc gctctaccct cactcctgaa agtatgcctt
    21001 cacgttcaga atgtcaatcc gcaggctttg cagtctgcga tcctgccagc aaatattctt
    21061 tgcgagacgt tacgcaataa tcacagagga aactatttta tccgcaagtc agcgatagac
    21121 tgaattcagg gcgaaaggag gtaagccgac gatttcagcg ggacgctgaa acgggaaagc
    21181 ccctcccgag gaaggggcct taaataagga aagggtcatg atgaagtgga tcatcatcgt
    21241 ggtgctctta gtcataagct tcccggctta ctaagactac cagggcgggg gaaaccccgc
    21301 tctaccctca ctcctgaaag tatgccttca cgataagatt gtcaatctgc aggctttgca
    21361 gtctgcgatc ctgccagcaa atattctttg cgagacgtta cgcaataatc acactggaaa
    21421 ctattttatc cgcaagtcag cgatagactg catgcatggc gaaaggaggt aagccgacga
    21481 tttcagcggg atgctgaaac gggaaagccc ctcccgagga aggggcctta aataagaaaa
    21541 gggttatgat gaagcacgtc atcatactgg tgatactctt agtgattagc ttccaggctt
    21601 actaagaaca ccagggggag ggggaaacct ttccctaacc ctcactcctg aaattatgtc
    21661 actccgccct tagtgtcaat cgacggctct ccgttcttgc gaggcgaata gcaaaatttt
    21721 ctgcgccgcc gggcctaatt ctgaaagttt cttcgtttgc tcttatttca ttattgcaat
    21781 cggtatgata ccggacctca ataattctct aagaaaggat ttcttatgag cgaacaaatc
    21841 acgtttgcca ccagcgattt tgccagtaac ccggaaccac gttgcccctg cattttgctg
    21901 ctggatgttt ctggttcgat gaacggtaga cccattaatg aacttaacgc cggattggtt
    21961 acctttcgcg atgagctgct tgccgacccg ctggccttaa aaagagtgga acttggtatt
    22021 gtgacgttcg gcccggtgca tgtggaacaa ccctttacca gcgccgctaa ttttttcccg
    22081 cccatcctgt ttgcccaggg ggatacacca atgggtgccg ccattaccaa agccctggat
    22141 atggtagagg agcggaaacg tgagtatcgt gcaaatggta tttcgtatta tcgcccgtgg
    22201 attttcctga tcaccgatgg tgcaccaacc gatgagtggc aggctgccgc caacaaagtg
    22261 tttcaggggg aagaagataa gaagtttgcc ttcttttcca tcggtgttca gggggcggat
    22321 attaagactc tggcacaaat cagcgttcgt cagcctttgc cactgcaggg attacaattc
    22381 cgtgaactgt ttagctggtt atccagttcg ctgcgttcgg tttcccgctc cacgccggga
    22441 acggaagttg tgctggaagc gccaaaaggc tggacgtcag tgtgagctgg cgtctggttt
    22501 atgcatccgc tgttggaacg tcgcacatta gcgctgattt accttgtcag gatgcctgcc
    22561 agatgcaggt tgcctggcta aatgaccagc agccattact ggtgatgttc cttgctgatg
    22621 gcgcaggcag tgtctcgcag ggtggcgaag gcgcgatgct tgccatcaat gaagcgatgg
    22681 cttatgtgtc gcaaaaagtg cagcacggtg aatttggact caatgatatt ctcgccacgg
    22741 atattgtgct gaccgttcgt cagcgacttt ttgccgaggc ggaagctaaa gaattagcag
    22801 ttcgcgattt tgcctgcacg tttctgggat tgatttcatc ggctaacggc accttgatta
    22861 tgcagattgg cgatggcggc gtggtggtgg attttggtca cggtctgcaa ttaccgctta
    22921 cgccgatggt gggtgaatat gccaatatga cgcatttcat taccgatgaa gatgtcgttt
    22981 ccagactgga aactttcacc agcactgagc gtgcgcataa agttgcagca tttacggatg
    23041 gtattcagcg actggcgtta aatatgctgg ataattctcc gcatgtgcct ttctttaccc
    23101 cgtttttcaa tggactggcg gcagcaacgc aggaacaact cgatttatta cctgaattgt
    23161 taaagcagtt tttgtcgagc ccagcggtaa acgagcgtac cgatgatgat aaaacgttga
    23221 cactggcgct gtggttacca tgaaacccac tttatatact gctactggtg agtgcgttac
    23281 gccaggccgt gaactgggca aaggtggcga aggcgcggtt tatgatatca atgagtttgt
    23341 cgatagcgtc gccaagattt atcacacgcc gccacccgcc ttaaaacagg acaaacttgc
    23401 ctttatggct gcgacagctg acgcgcagtt gttgaattat gtcgcctggc cgcaggcaac
    23461 gcttcacggt ggacgaggcg gaaaagttat cggttttatg atgccaaaag tttctggtaa
    23521 agaaccgatt catatgatct atagcccggc acatcgtcgc cagagttatc ctcattgtgc
    23581 gtgggatttt ctactctatg ttgcgcgcaa tattgcttca tcttttgcta cggttcacga
    23641 gcacgggcac gtcgtggggg acgtaaacca gaacagcttt atggtaggtc gcgacagcaa
    23701 agtggtgttg atcgatagtg actcctttca gattaacgcc aatggcacac tgcatttatg
    23761 cgaagtcggc gtgtcgcatt ttacgccgcc agagctacaa accttgccat catttgtcgg
    23821 ttttgaacgt accgcgaatc acgataattt tggccttgcg ttgctgattt ttcacgtctt
    23881 gtttggtggg cggcatcctt attctggtgt gccgcttatc tctgatgcgg gtaatgcgct
    23941 ggagacggat attgcccatt tccgttatgc ctacgcgtca gacaaccagc gacgtggttt
    24001 aaaaccgccg ccacgatcga ttccgctgtc gatgttaccg ggcgatgttg aagccatgtt
    24061 tcagcaggca ttcacggaaa gtggcgtggc aaccgcgcgt ccgacggcaa aagcgtgggt
    24121 agcggcactg gatttactac gccaacagtt aaagaaatgt accgtttcgg caatgcatgt
    24181 ttaccccggt catttgactg actgcccgtg gtgtgcgctg gataatcaag gcgttatcta
    24241 ttttattgat ctcggcgaag aggtcattac caccagtggt gattttgtgc tggcgaaagt
    24301 ctgggcgatg gtgatggcgt cagtagcacc gccagcattg caattgccat tacccgatca
    24361 tttccaaccg actggcaggc cgcttccttt aggcctgtta cggcgcgaat acatcattct
    24421 gattgagatc gcactgtcag cgttatcgct gttgctttgc ggccttcagg cagaaccgcg
    24481 ttatattatt ttggttcctg tgctggcggc tatctggatt attggcagtc tgacaagcaa
    24541 agtttataaa gcagaaatcc agcaacgccg tgaggcattt aatcgcgcga aaatggacta
    24601 tgaccattta gtcagccaga tccaacagtt gggcgggctg gaaggtttta tcgccaaacg
    24661 ggcgatgctc gaaaaaatga aggacgaaat tctcgggtta ccggaagagg agaaacgcgc
    24721 tctggcagca cttcatgaca ctgcaaggga acggcagaag cagaagtttc tggagggatt
    24781 ttttattgat gctgcctcta ttcccggcgt tggccctgcg cgtaaagcgg cgttacggtc
    24841 ttttggtatt gaaacagccg cggatgttac ccgtcgtggg gttaagcaag ttaaagggtt
    24901 tggtgatcat ctgacccagg cggttatcga ctggaaagcg agctgtgaac gccgttttgt
    24961 gttcaggccg aacgaagcgg taacgcctgc agaaagacaa gcggtaatgg cgaaaatggc
    25021 cgccaaacga catcggctgg aatcggcgtt gactgtcggc gcgacagagt tgcagcgatt
    25081 ccgccttcat gctccagcac ggaccatgcc gttgatggaa ccgttacgtc aggcggcaga
    25141 aaaactggct caggcgcagg cagatttaag ccgctgctga gccatttttc aattaacgaa
    25201 acaccacttc cgcccagcgt gccagcccgg cggtgacgga gccaaagtca tcgccgcctg
    25261 caatcggaat gcccggcaac tgttctgcca gcgctttttt aatcagcgga gaacgggcgc
    25321 ttccgccggt caggtagata acgtccggtt tttcctgggc gttatccagc gccagttgca
    25381 cctgttcctg aatccgcgcc agtggctggc tgagggcgct ttccagccct tgctggctaa
    25441 tcagcgtagc cagttcatcg ctgataaacg gcagtgaggc gcgggtttcc gctacgcttg
    25501 aaagcgcaat tttgcactct tctgcgctac gcaccaggcg atagcttaaa cgctgacgcc
    25561 agactttctg tagcagggcc actttttccg gttcgcgggc atcgcgtacc agatcgttaa
    25621 gcagacgacc gttggcacta ctgtagaaat cactctgcgc aggtacgtcg ttgatggcaa
    25681 ccgcattcca ccacggcaga atcggcaggg cgatgccttt ttcggtttcg ccacccatac
    25741 ccagcagtgg catcaggttt ttaaacgcca gtgcgatatc cagatcgtta ccgccaatac
    25801 ggcaaccact gtgacccagc aggctggctt cacgatcgag acgcgaacgc cactgcggcc
    25861 ccatcagcag caatgaacag tcagtcgtac caccgccgat atccaccacc agtacccgtt
    25921 tttcttcctg taaggtggct tcgtaatcca gcccagccgc taccggctcg tactggaata
    25981 ccacgtccct gaatccggca cgcttcgccg cgcgttccag aatcccttgc gcctgggcgt
    26041 ttgcttcatc gccgcccagc ccctggaagt tgatcgggcg accaatcacc gcctgagtaa
    26101 tcgcttctgg cagctgcgct tgcgcctgct ggcgaatgtg caacatcatt gcgcagacca
    26161 gatcctcaaa cagcgctacc tgctgcggtt ttaagccgct ggcaccgagg aacgattttg
    26221 gtgatttcac aaaccacact tcttctggat catcaatgta ctgtgccagt gaggaaagac
    26281 cgaactgcac gcttttcgcc gtaacatcga tatcttcttc gcgattataa cgaatcgccc
    26341 gacgcaacag tgcctgcgtt tcatcgtcgt ctgccggaac atcatgatgg cggtacagcc
    26401 attcgcttac cgcttcacgc gttggcgcgc aaagcattga aggcagcagc gtgctgtcgt
    26461 tttccatttt tagcaagtgc ggtttaccgt cacgcatgac cgctactgaa cagtttgctg
    26521 taccgtaatc aaaaccaata aacacgaaat aatccccatg ccggtgaaga agggcgtgac
    26581 tttagcgaaa tgttgccgtc gcgacaaccg gaatatgaaa gcaaagcgca gcgtctgaat
    26641 aacgtttatg ctgaaagcgg atgaataagg agatgcgatg tataccctga actggcagcc
    26701 gccgtatgac tggtcgtgga tgttgggatt tctcgccgcc cgtgcagtga gtggtgtgga
    26761 aacggtcgct gacgattatt atgcccgtag tctggcggtg ggcgaatatc gcggcgtggt
    26821 gactgctatt ccggatatag cccgccatac tctgcacata aatttaagtg cagatttaga
    26881 acccgttgcc gctgagtgtc tggcgaaaat gagccgcctg cttgatctgc aatgtaaccc
    26941 gcagattgtt aacggtgcgt tgggcaagtt aggcgcggcg cggcccggat tgcgtttacc
    27001 cggcagtgtt gatgcttttg agcaaggcgt gcgggcgatt ttaggccaac tggtgagcgt
    27061 ggcgatggcg gcaaaattga ctgccaaagt ggtacagctt tatggcgaac ggctggatga
    27121 ttttccggag tatatctgct tccccacgcc tcagcggctg gcagcagcag acccgcaggc
    27181 attaaaagcg ttaggtatgc cgttgaaacg ggcagaggcg ctgattcatc tggcaaatgc
    27241 ggcgctggag ggcaccttac caatgacaat accgggcgat gtggagcagg cgatgaaaac
    27301 gctgcaaact tttccgggta tcgggcgctg gacggcgaat tattttgctt tgcgtggctg
    27361 gcaggcgaaa gatgttttcc tgccggatga ttatctgatt aaacagcgat ttccgggaat
    27421 gacaccggcg caaatccgcc gttatgccga gcgctggaag ccctggcgtt cttatgcgct
    27481 gttgcatatc tggtatacgg aaggctggca accagacgaa gcatgacgtt ggtcgccgga
    27541 tgcggtgcga gcgccttgcg gaatgagcga tttgtaggcc tgataagacg cgttagcgtc
    27601 gcatcaggca atgggcaaac gctccctcag ttaatcgcga aataactact attcatcagc
    27661 aaatccagcg gttgggcatc ggcaatcacc tcaccataaa tcagatcgac gccgatgcca
    27721 gaaagcgtat ccatcactaa gggtaaaacg actggcccgg cgatggtttt catcccgagt
    27781 cgctgagcgt gcccctgaat aatcgtaatc agcatctcat ccatcaaatt accctgcacg
    27841 ttggcgcata actcaccatc aagtagcagg taatctgcca tattcgcttt cagcgagttg
    27901 aagatttgca gatcgcggcc cacctgactg aatacgatcc ggcatccccc cagccgcagt
    27961 ttttgcacgc tttctgcgtg atcgaaaatc gcttctgccg gaataatcag atgtaataac
    28021 cgtggtggta gagggctatt ttccagctgc tcaagcagat cattcaccag cgtggcgcta
    28081 ctcaaaccgg caacggaaag ggggagggcg atgcttatgc ctttactggc aaccgctttt
    28141 gcggcctgct ggagaaattc gtggaatacc cggcggtcaa gagcatggct aagcgccgga
    28201 tcgctgaagc tacgacgaaa tgtttgttca tcaataatct cgccttcgca actccagagc
    28261 ttaagtgaaa tcagccacaa attacgcgct tccgggatcc gtggcgaagc gacaccgtgg
    28321 gcgagcatca tcaactgatt ctctttaatc atccgccact gttcatcaag cgacatcgcc
    28381 gcccgttcgc tatgtgcggc agcttgctgc ggttcgtaaa ccgtcacccg gccccggcca
    28441 ccatttttgg aggcataaca ggcgatatca gcctgcgaca tcacttctgc cgcctgatga
    28501 ttgttgtcat cgatcaaggt aatcccggcg ctggcaccta cccgatgtac ccggccttcc
    28561 catataaagt gatagtcatt cacagcactg ataatacgtg tagcgataaa acgagcgctt
    28621 tcaacattgc agtctggcag cagcagacca aattcatcac cgccgagccg cgccagcacg
    28681 tcactggagc gcagcatact cagcatcaac gacgccagtt cgcgcagcaa agcgtcgccc
    28741 gccgcatgcc cggcgctgtc attcaccgct ttaaagcgat caagatcgat aaacaccagt
    28801 gcatgtcgct gatgcgtact gtttaccgtt tgcagcagga tacgcagttg tttctcaaaa
    28861 ctggcgcggt tggcgagatg cgtcagtgca tcatgggagg cgctgtagct cagctggcgc
    28921 agcattttgc gtgattcggt aacgtcctga atcaccagta ccgagcctat attgctgccg
    28981 tccagagtac ttaacggcgt aatactgtaa tgcacgtcgt agctgccgcc gctccgacag
    29041 tgcaacacca catcttgttc gatcgcggaa cgtgaggtgt cggcactgta aatgttctcc
    29101 attaatggtc cgttgtcgcc aaaagtaata tgcaacaccg tcaggagcgg aacacctaac
    29161 gcttcttcct gcgtccagcc gctcatcttc tccgccactg gattcataaa ggtaattttc
    29221 atcgccatat caatacagac cacggcttcg ccgatggagt ccagcgtaat gtgcaggcgc
    29281 tctttttcct gaaacaatgc ctcgttaagc tgtttaacct cagtcatatc catattaatg
    29341 ccgagcaggc gttcgacttc gccttcttta ttcagtaccc ggttggcgag ggcgcggata
    29401 tggcggatgc catctttcac ggcaatgcga aattccagtt taaagggcga gcgtgattgc
    29461 aacgaatcac gaatcacttt ttcggcgtgt tggcgatctt ccggcagcac gcactcgtac
    29521 cacacctgcc agttcggttt gatatgcgga ggaatttcat acagctcgaa catccgctta
    29581 tcccagctaa aaatattcgg cttcagttcc cactcccaga tgccaatccc gcccgcttcg
    29641 ttagccagcg tgatgcgttc catcagttgc tgattcacct gttcggtgcg ttttagctcg
    29701 ttaatgtctt caatctgagc gataaaatag agcggcgtgc catccgtgtg gcgcaccaga
    29761 gagacggcaa gcaacgccca gacaacatcg ccattgcggt tgtagtaacg tttttccatt
    29821 gaataggtgt ttatttcacc gcttatcagc ttttcaacct gttggagatc tttattgaga
    29881 tcctccggcc aggtcagttg ctgaaaggtg agtccgcgca gctcttcctg actgtaaccg
    29941 agaaactggc agagtgcttt gttggattgc agccattgtc cctcggtgcc cactaatgcc
    30001 atgccgatgg cggaatattc catggcgttc cgaaaacggg tttcgctttc ggaaatgtgt
    30061 ttgcgttccg cacgaaaggc atacatcacc atcgtcatga tgttggcggg cagcaggatc
    30121 agcaaaaacg gtagccacgg catatggctc atcaggtacg tacgcggcgt agcaagcagg
    30181 gagggatccg cggccatcat cagcgacacc atcatcaccg tggtaaggaa gatcaaaaag
    30241 gcttccattc gtggcaggcg cacggcgctc cacatcaaca gcacaataat gaaagtaaaa
    30301 ggccacggca gatacagcat cgaaagccag cttaacgtca gtgtgatggc taacgtgagc
    30361 agcgactcaa aaagcaaccg tgggttgcga tggcgcagca gatagtgtgg tttaaataac
    30421 aatcccagcg gcaccagtgc cagagcgccg atggattctg acagtaccca tatcaaaaat
    30481 gccctgatga ggatcgtctc cgggcgtcag caggacaacc agaacacccc ccaacagagg
    30541 tggaacaatg gcgctgccga gtgccagacg cagccagtca gccagatttt gcaaggggtt
    30601 ataccacggc agcaatttac gtagcagcac tgccccgacc acggcttcaa caatattgat
    30661 ggtcgtgcag gtcatgttca gcgagctggt ggaaaaaagc aggatggatg cggcgatatt
    30721 tcccagcgag cagctcagcg caatccctgg ccacatgcgc ccggcatggc gataaaacgc
    30781 caccatcatg atggacgtcg ggaaccacag tggggcgagt tgggtgccaa actgcgaaag
    30841 ctcaagcgag aaaagggtga agataaacga gactaaacct aaactgacca ggtgaagcag
    30901 cgggtggggc agggcaatta atacatgctg tgattgtttg ctcattaccg ctttatccgc
    30961 aaggccaggt cgccagtatt cgtttctgtg tttatgctag tacaaaagtt ttacatttta
    31021 tatgcgaatt gctcataaag tgacgtaaag gcggataatt tgcgcaactg cgtataacaa
    31081 tttttacctt gtataaaact gatcaacgta atttgccacc gggaattctt cgtcaacaat
    31141 taactctgag cgcgcggcaa cgctattcga ctggtatcag gcggatgaaa tccctataat
    31201 tgccgcgttt ggcgcttcgt cgcccccttc ctaacatcca ggttaatcag gtcgctaaat
    31261 atatgactga tcagtctcat cagtgcgtca ttatcggtat cgctggcgca tcggcttccg
    31321 gcaagagtct tattgccagt accctttatc gtgaattacg tgagcaagtc ggtgatgaac
    31381 acatcggcgt aattcccgaa gactgctatt acaaagatca aagccatctg tcgatggaag
    31441 aacgcgttaa gaccaactac gaccatccca gcgcgatgga tcacagcctg ctgcttgagc
    31501 atttacaagc gttgaaacgc ggctcggcaa ttgacctgcc ggtttacagc tatgttgaac
    31561 atacgcgtat gaaagaaacg gtgacggttg agccgaagaa ggtcatcatt ctcgaaggca
    31621 ttttgttgct gacggatgca cgtttgcgtg acgaacttaa cttctccatt ttcgttgata
    31681 ccccgctgga tatctgcctg atgcgccgca tcaagcgtga cgttaacgag cgtgggcgtt
    31741 caatggattc cgtgatggcg caatatcaaa aaaccgtgcg cccgatgttc ctgcaattca
    31801 ttgagccttc taaacaatat gcggacatta tcgtgccgcg cggcgggaaa aaccgcatcg
    31861 cgatcgatat attgaaagcg aaaataagtc agttctttga ataagcttga taaattgtgt
    31921 accgttcagt gataacctgg tatgcccttg acgtaattgg cgttaaggga gtgatgcgcg
    31981 aaaggagaaa atgccatgcg tctgtgtgac cgagatattg aagcctggct tgatgaaggc
    32041 cgtttgtcga tcaacccacg tccgccagtg gagcgtatta acggcgcgac ggtggatgta
    32101 cgcctgggca ataaatttcg taccttccgt ggtcacacgg cagcgtttat cgatctgagc
    32161 ggtcccaaag atgaagtgag cgccgcgctt gaccgcgtga tgagcgatga gatcgttctc
    32221 gatgaaagcg aggcgttcta tcttcaccca ggagagctgg cgctggcggt gacgctggag
    32281 tcggtgacgc tgccagccga tctggtgggc tggctggacg ggcgttcctc actggcgcgt
    32341 ctggggctga tggtgcacgt caccgcgcac cgtatcgatc cgggctggtc tggttgcatt
    32401 gtgctggagt tctacaactc cggtaagctg ccgctggcgc tgcgtccggg catgttaatt
    32461 ggtgcgctga gctttgagcc gctttccggc ccggcggcgc gaccttacaa ccgccgtgaa
    32521 gatgcgaagt atcgcaacca gcagggcgcg gtagccagcc gaatcgataa agactaattc
    32581 aggcccattg aggataccat gagacgattt ctgacgacgc tgatgatact cctggtcgtg
    32641 ctggtggccg ggttatctgc gttagtgttg ctggtgaatc cgaatgattt ccgcgactat
    32701 atggtcaagc aagttgctgc acgtagcggt tatcaattgc agctcgacgg gccactgcgt
    32761 tggcacgtct ggccgcagct tagtatcctc tccgggcgaa tgtctctcac cgcccagggc
    32821 gcgagccagc cactggttcg cgccgacaac atgcgcctgg acgtggcgct tttaccacta
    32881 ctgagtcatc aactgagcgt taagcaggtg atgctaaaag gggcagtgat ccaactgacg
    32941 ccgcagacgg aagcggtgcg cagtgaagac gctccggttg caccgcgcga caataccttg
    33001 ccggatctgt cagacgatcg cggatggtcg tttgatatat ccagtcttaa ggtggcggac
    33061 agcgtgctgg tgttccagca tgaagatgac gagcaggtga caatccgcaa tatccgcctg
    33121 caaatggaac aagatcccca acatcgtggc tcatttgagt tctccgggcg ggttaatcgc
    33181 gatcagcgcg atctcacgat atcccttaac ggtacggtag atgcttctga ttatccgcat
    33241 gatttaacgg cggctattga acaaattaac tggcagttgc agggtgccga tttaccaaaa
    33301 caaggtattc aggggcaggg gagtttccag gcccagtggc aggagtcacg taaacgcctt
    33361 tcatttaacc aaattagttt gaccgccaat gatagtacgc tgagcgggca agcacaggtc
    33421 acgctgacag agaaaccgga atggcagctg aggctgcaat tcccgcaact gaatcttgac
    33481 aacctcatcc cgcttaatga aaccgcgaat ggtgaaaacg gtgccgcgca gcaggggcag
    33541 agccaatcaa cgttgccgcg cccggttatt tcttcgcgta ttgatgaacc ggcctatcag
    33601 ggactgcaag gctttacggc tgatattttg ttgcaggcca gtaacgtgcg ctggcgcgga
    33661 atgaatttta cagatgttgc cacgcaaatg accaacaagt cgggtttgct ggaaattact
    33721 caactacagg gcaagcttaa cggtggacag gtttcactgc cgggcacgct ggacgcgaca
    33781 tcaataaatc cgcggataaa cttccagcca cggctggaaa acgttgagat tggcaccatt
    33841 ctgaaggcgt ttaactatcc gatttcgttg accggaaaaa tgtcattggc tggtgatttc
    33901 tccggtgctg acatagatgc cgacgcattc cgccacaact ggcaaggaca ggcacatgtc
    33961 gaaatggccg atacgcgcat ggaagggatg aacttccagc agatgattca gcaagcggta
    34021 gaacgtaatg gtggtgatgt gaaggccgct gaaaacttcg ataacgtaac gcgtcttgac
    34081 cgctttacca ccgatttgac gttgaaggat ggcgtcgtga cgttaaacga catgcaaggt
    34141 caatcgcctg tgctggcgct tacaggggaa ggcatgttga atctggcagc tcaaacctgc
    34201 gacacccagt ttgatattcg ggtcgtgggt ggctggaacg gggaaagcaa actgattgat
    34261 ttcctcaaag aaacgccagt accgctgcgg gtttatggca actggcagca actcaattac
    34321 agtctgcaag tggatcagtt actgcgcaaa catctacagg acgaagcgaa acgtcgcctg
    34381 aatgactggg ccgagcggaa taaagattcc cgcaatggca aagatgtgaa gaagttgctg
    34441 gagaagatgt aagacaagtt atatagatgg cggcatcaat ttgtcgccat ctgtatttat
    34501 agcactaagc agcatgtgga tattgaactt caatacgttg aatatgtagt cctgcatgag
    34561 ccatcatggt gatcaattta tcgatactaa agatctggat tttacctctg gcaagttcag
    34621 agatgcgagg ttgagtgacc cctaaagcag aagctgcctg agcctggcta aacccttgtt
    34681 gttcaatcca gttattaatg atcgttacaa gctcggcacg gatccgcata ttttccgctt
    34741 gttcaggggt gtcactcacc gcatcccaca cactatcaaa ggtttcaatt ttcatttttc
    34801 cgctcctgaa caagttcttt atagcgtcgt ttggctaaat caatatccga ttgactggta
    34861 gtctgtgttt tcttttgaaa acaatgcaat acgtaaaccg cttcttcaaa ttttgcgaca
    34921 tacatgacgc gatagatccc atcagcatcg cgagtacgaa tctctcgcac accagggccg
    34981 atagttgaaa aaggtttcca gtcgtatggg tttaaccctt gttggatcct gtcgagttga
    35041 tagccaattt cctgtcgaga cgattctgga aatgcgcgta aatcgtcaag actggtatca
    35101 agaaaagcca gcttttttct cattactcca tccttagatt atactaaatt ttgtataaat
    35161 caaatgcgca gtttgaacct gcgcatttga tgttttatca cacctcgtac tccatctcgc
    35221 catccttacg cagcggtata atctgcacct tctgcacgcg atggctttcc acctgcaacg
    35281 ttttaagcaa ataatccccc acctgaactt cttcgccagg ttttggaata cgctgcaaat
    35341 attccatcaa tagcccggca atggtgtgat attcacgttt ttcatccagc ggcagcggca
    35401 catattgcac cagatcttcc agcggcatat gaccattcgc cgtccaggaa ccgtccgcat
    35461 tcttctgaat atcatggcgg gcgtcgatct cttccacttc gttcggtaag ttaccggcaa
    35521 tggtttcagt gacgtcactt aatgtcacaa tcccttccac cgagccaaac tcatccacca
    35581 caaaagcaaa gtgtgtgcgg gcattacgga actgctccag ggcaggtaac aacggcaagg
    35641 tttccgggaa caccagcggc tggcgaatta acacccgcag gttgagcggt tcgccgcgca
    35701 gtgattgttg taacaggtcg ataacgtgaa caacaccgag caaatcttcc gcgtcatcgc
    35761 catcagtaac caccagccgc gtatgctgat ttcgctccag caactggcgg atctcgtctt
    35821 ccggcgcgtt gagatcgata tgctcaatat cgtggcgcga cgtcataatg ctgctgacgg
    35881 tacgctggtt aagattaagt acccgctcaa tcatccgccg ttcctgcgga ttaaagatct
    35941 gctggttacc atgatccacc agcatggacg cggtttcggc gtccagttcc gcatcctctt
    36001 tttgcccgct aagcaggcgc attaccgctt cggtagtccg ctggcgcagt gtctggttag
    36061 cggaaagaaa acgtcgccgg ttaaagatag ccaactgatt gagcgcctcg atcatcaccg
    36121 agaaaccaat ggcagcgtac aggtagcctt tcggaatgac gaagccgaaa ccttctgcca
    36181 ccaggctaaa gccaatcatc aacaggaagc ttaagcagag aataacgatg gtcgggtgac
    36241 tgttaacaaa ttgcgttaac ggcttgctgg ccatcaacat caggctgata gcgataacca
    36301 cggcggccat catgaccagt aaatggtcga ccatcccgac ggcggtaatc actgagtcga
    36361 gtgagaagat ggcgtccagt accacaattt gtgtcaccac cccccagaac ttcgcacctt
    36421 tgcgttgtgt gggattattg ctgtctttcc cttccagccg ttcgttcagc tccatcgtgg
    36481 ctttgaacaa caggaagaaa ccaccaaaca gcatgattaa gtcgcgggcg ctaaaggtaa
    36541 acgagcggaa gctgaacagc ggttgagtca gggtgaccag ccaggagatt gacgccagca
    36601 gtaacaggcg cattaacatc gccagcaaca gcccggtaac ccgtgcgcgg tcgcgctgct
    36661 tcggcggtag tttttcggcg aggatggcaa taaagaccag gttatcaatg ccgaggacca
    36721 gttcgatcac aatcagcgtg attaacccgg cccagataga cggatcggca atccattcca
    36781 tagtaagtac aaaacctatt cgttgtatga caaatgtcac aattcgatca tggataaatc
    36841 ggggcaaaaa tgcaatgttg cagcactatt tatctcatat tatttgaggg taataacctc
    36901 acattatccc tgaattaaaa gtggtaataa taaaacatta tttaagaaat atcgcatgtg
    36961 gagacgcaat attaaatatt gtcaacctaa agaaactcct aaaaaccata ttgaatgaca
    37021 cttaatataa ttcttaaaaa tagccaattg ccgaattgtt accttgcctg ctattccgtt
    37081 agctgtaaca cttcctcctg cattattgga gagccaatat tcaattacca taatgtcacc
    37141 ttatattcgg aagttaattt ccgcaataac cctttctcat caacgattgt atgcagagag
    37201 ggtttgaaaa attaagctaa atcagtgtat tggtagctaa aaagccaggg ggggtagcgt
    37261 gtctggatgc ctgaaagacc agtctgaatt attctgtcaa tagcctgcgg atgaaatcaa
    37321 tttttttagg actgatgcca gttaaatttt tattcaatta actgcaaggt aaatgtgcat
    37381 ggcgacattg ctattaatag tgcacaggat aattactctg ccaaagtgat aaataaacaa
    37441 tgatgaaatc caaaatgaaa ttgatgccat tattggtgtc agtaaccttg ataagcggtt
    37501 gcacagtact tccgggcagc aatatgtcga cgatgggcaa agacgtcatc aaacagcagg
    37561 acgctgattt cgatctcgac aaaatggtga atgtttatcc gctgaccccg cgcctgattg
    37621 accaattacg cccacgcccg aatgtagcgc gccccaatat gacgctggaa agtgagatcg
    37681 cgaattacca gtatcgcgtc gggccggggg acgttcttaa tgtcaccgtc tgggatcacc
    37741 cggaactcac cacgccagcc ggtcagtacc gcagctccag cgacaccggc aactgggtac
    37801 agcctgacgg cactatgttt tacccgtata tcggcaaggt tcacgtagtc gggaaaacgc
    37861 tcgctgaaat ccgcagtgat attaccgggc gcttagcgac gtacatcgct gacccgcagg
    37921 tggacgttaa tatcgccgcc ttccgctcac aaaaggccta tatctcaggt caggtgaata
    37981 aatctggtca acaggcgatc accaacgtac cactgaccat tctcgacgcc atcaacgccg
    38041 caggtggcct gaccgacacc gctgactggc gcaacgtggt gctaacacac aatggtcgtg
    38101 aagagcgaat ttctttgcag gcgctgatgc aaaacggcga cctcaatcag aaccgactgc
    38161 tttaccccgg cgatattctc tacgtgccgc gtaatgatga tctgaaagtg tttgtgatgg
    38221 gtgaagtgaa gaaacagagc accctgaaaa tggactttag cggcatgacc ctgaccgaag
    38281 ccctgggcaa tgctgaaggc atcgacatga ccacctccaa cgccagcggc atctttgtca
    38341 ttcgtccgct gaaaggcgag ggcgggcgta acggcaaaat tgccaatatc taccagctgg
    38401 atatgtccga tgccacgtcg ctggtgatgg cgacagaatt ccgcctgcaa ccttatgacg
    38461 tggtgtatgt caccaccgcc ccggtttccc gctggaaccg tctgatcaat cagttgctgc
    38521 caactattag cggtgtccgt tacatgacgg atacagccag cgacattcat aactggtaat
    38581 cgtcatgttt aacaacatct tagttgtctg tgtcggcaat atttgccgtt ccccgacggc
    38641 ggaacgctta ctgcaacgtt atcacccgga gctgaaagtg gagtccgctg gactcggcgc
    38701 gctggtcggt aagggcgctg atcctaccgc tatcagcgtc gccgcagaac atcaactgtc
    38761 tctggaaggc cactgtgcac gacaaatcag ccgccgcctg tgtcgcaact atgacctgat
    38821 tttgaccatg gaaaagcgcc atatcgaacg cttatgcgag atggcaccgg agatgcgcgg
    38881 caaagtgatg ctgtttggtc actgggataa cgaatgtgaa atccccgatc cgtatcgcaa
    38941 aagccgggaa acgtttgcag cggtgtacac attacttgaa cggtctgccc gccagtgggc
    39001 gcaggcattg aacgcagagc aggtataaga atgacagaaa aagtaaaaca acatgccgct
    39061 ccggtaacgg gcagtgatga aatcgatatt ggtcgcctgg tcggcaccgt cattgaagcg
    39121 cgctggtggg tgattggcat caccgctgta ttcgcccttt gtgccgtggt ttacaccttc
    39181 ttcgccacgc cgatttatag tgccgacgca ctggtacaaa tcgagcaaag cagcggcaat
    39241 tcgttagtgc aggatatcgg atcggcgtta gccaacaaac cgcctgcatc ggacgccgag
    39301 atccagttga ttcgttcgcg cctggtgctt ggtaaaacgg tggatgatct cgacctcgat
    39361 attgcggtga gcaaaaacac gttcccgatt ttcggtgcgg gctgggatcg cctgatggga
    39421 cgccagaacg agacggtgaa agtgactacc tttaaccgtc cgaaagagat ggaggatcag
    39481 gtgtttacgc ttaatgtgct ggacaacaaa aactacaccc tgagcagcga tggcggcttt
    39541 agcgcccgtg ggcaagcggg ccagatactg aaaaaagaag gcgtcacgct gatggttgaa
    39601 gccattcacg cccgcccggg cagtgagttt accgtcacca aatactccac gctggggatg
    39661 atcaatcaac tgcaaaacag cctgacggta acggagaacg gcaaagacgc aggcgtactg
    39721 agcctgactt ataccggtga agatcgcgaa cagatccgcg acattcttaa cagcatcgcc
    39781 cgtaactatc aggaacaaaa tattgagcgc aaatcggcgg aagcgtcgaa aagcctcgct
    39841 ttcctcgcgc aacagttacc ggaagtacgt agccgccttg atgttgccga aaacaaactg
    39901 aatgccttcc gtcaggataa agattctgtt gatctgccgc tggaagcgaa agcggtgctc
    39961 gattcgatgg tgaacatcga cgcccagttg aacgaactga cctttaaaga ggcggaaatc
    40021 tccaagctgt acaccaaagt tcaccccgcg taccgcacgc tgctggagaa acgtcaggcg
    40081 ctggaagacg aaaaagccaa acttaatggt cgcgtaacgg cgatgccgaa aacccagcag
    40141 gaaattgtcc gtctgacccg cgatgtcgag tctggtcagc aggtctatat gcaactgctg
    40201 aataaagagc aggagctgaa aatcaccgag gccagcaccg tcggcgatgt gcgcattgtt
    40261 gacccggcaa tcactcagcc tggtgtgcta aaaccgaaga aagggctgat tatccttggg
    40321 gcgattatcc ttggcctgat gctctctatc gtgggggtgc tgctgcgctc gttgtttaat
    40381 cgcggcatcg aaagcccgca ggtgctggaa gaacacggta tcagcgtcta tgccagcatc
    40441 ccgctgtcgg agtggcagaa agcgcgcgat agcgtcaaaa ccatcaaagg gattaaacgc
    40501 tataaacaga gccagctact ggcggtgggg aatccaaccg atctggcgat tgaagccatc
    40561 cgcagccttc gtaccagttt gcacttcgcg atgatgcagg cgcagaacaa tgtgttgatg
    40621 atgaccgggg ttagcccgtc aatcggtaaa acctttgtct gcgccaacct ggcggcggtg
    40681 atcagccaga ccaataaacg cgtgttgttg atcgactgcg atatgcgcaa aggctacacc
    40741 catgagctgt tgggcactaa taacgttaat ggcctgtcgg aaattctgat tggtcagggc
    40801 gatattacta cagctgctaa accgacctct attgccaaat ttgacctgat cccgcgcggt
    40861 caggtaccgc caaatccttc tgaactgttg atgagcgaac gctttgccga actggtgaac
    40921 tgggcgagta aaaactacga cctggtgttg attgatacgc cgccgattct ggcagtgacc
    40981 gatgcggcaa ttgttggtcg tcatgtcgga accacgttaa tggtggcgcg ttatgcggtc
    41041 aacacattga aagaagtgga aaccagtctg agccgctttg agcaaaacgg tattccggtg
    41101 aaaggggtga ttctgaactc catcttccgc cgcgccagcg cgtatcagga ttatggctat
    41161 tacgaatacg aatataagtc ggatgcgaaa taacgaggcc tgcattcgca ccgccccgta
    41221 ggccggataa ggcgctcacg ccgcatccgg caagcaaacc agctcataag ccgggagtac
    41281 aacctatgaa agacaatccg ctgatctcaa tctatatgcc gacctggaac cgccaacaac
    41341 tggcgattcg ggcgataaaa tcggtcttgc gccaggacta cagcaactgg gagatgatca
    41401 tcgtggatga ttgctccacc tcctgggaac aactgcaaca gtacgtcacc gccctcaacg
    41461 atccgcgtat tacctacatt cacaacgaca ttaacagcgg ggcgtgcacg gtacgtaacc
    41521 aggcgattat gctggcgcaa ggggaataca ttaccgggat cgatgacgat gatgaatgga
    41581 cgcccaaccg cctgagcgtc ttcctcgccc ataaacagca actggtcact cacgcctttt
    41641 tgtacgctaa cgattacgtt tgccagggcg aagtttattc ccaaccggca agcttaccac
    41701 tgtatcccaa atcgccgtac tcacgccgcc tgttctataa acgcaatatc attggcaatc
    41761 aggtctttac ctgggcatgg cgtttcaaag agtgtttgtt cgataccgaa ctgaaagcgg
    41821 cacaggatta cgacatcttc ctgcggatgg tggtggagta tggcgaaccg tggaaagtgg
    41881 aagaggcgac gcagatcctg catatcaatc acggtgaaat gcagatcacc tcgtcaccga
    41941 aaaaattctc cggatacttc cacttttacc gcaagcacaa agacaaattc gaccgtgcca
    42001 gtaaaaaata tcagctgttt accctctacc agatccgcaa taaacgtatg acctggcgca
    42061 ctttgctgac gctgctgtcg gtgcgtaacg gcaaacgtct ggctgatggt attcgggggc
    42121 gctaaatatg ctggaagatc tacgcgccaa cagctggagt ttacgcccgt gctgcatggt
    42181 tcttgcctat cgcgtcgctc atttttgttc ggtgtggcgc aaaaagaacg ttctcaacaa
    42241 tctttgggcg gccccgctgc tggtgctgta tcgcattatc accgaatgct ttttcggtta
    42301 tgaaatccag gctgccgcga ccattggccg ccgctttact atccatcacg gttacgccgt
    42361 agtgatcaat aaaaacgtgg tagcggggga tgatttcacc attcgccacg gcgtcactat
    42421 cggcaatcgt ggtgccgata acatggcatg tccacacatt ggcaacggcg tcgaactcgg
    42481 tgccaacgtc attattcttg gtgatatcac gcttggtaac aacgtcaccg tgggcgcggg
    42541 cagcgtggtg ctcgactctg tcccggacaa cgcgctggtg gtgggagaaa aagcgcgagt
    42601 gaaggtaatt aaatgaatat tctgcaattt aatgtgcgac tggcggaagg cggggcggca
    42661 ggtgtggcgt tagatctcca ccagcgtgcg ctgcaacagg ggctggcgtc acattttgtc
    42721 tacggctacg gcaaaggcgg caaagagagc gtcagccatc agaactatcc gcaggtcatc
    42781 aaacatacgc cgcggatgac cgcaatggcg aatattgccc tgtttcgtct gtttaatcgc
    42841 gatctgtttg gcaatttcaa tgagttatat cgcaccatta ctcgtacacc gggtccggtg
    42901 gtcctgcatt ttcatgtgct gcacagctac tggctaaatc ttaagggcgt ggtgcgcttt
    42961 tgcgaaaagg tgaaaaacca caaaccggat gtcactctgg tctggacgct gcacgaccac
    43021 tggagcgtta ccgggcgctg cgcctttacc gacggttgtg aaggctggaa aacgggctgc
    43081 cagaaatgcc cgaccttaaa taactatccg ccggtgaaga ttgatcgggc gcaccagctg
    43141 gtggcgggca aacgccagtt attccgtgag atgctggcgc tgggctgtca gtttatttcc
    43201 cccagccagc atgtggctga cgctttcaat agcctgtacg gtccagggcg ttgccggatt
    43261 atcaataacg gtattgatat ggcaaccgag gcgattctgg cggatttgcc tccggtgcgc
    43321 gaaactcagg gcaagccgaa aatcgcggtg gtagcgcatg acctgcgtta cgacggcaaa
    43381 actaaccagc aactggtacg cgagatgatg gcgctgggcg acaaaatcga actgcatacc
    43441 tttggtaagt tctcgccgtt caccgctggc aacgtggtta atcacggctt tgaaactgac
    43501 aagcgcaagt tgatgagcgc gctcaatcag atggatgcgc tggtgttcag ttctcgcgtc
    43561 gataactacc cgctgatttt gtgtgaggcg ttatcaattg gcgtgccggt gattgccacc
    43621 catagcgacg cggcgcgtga agtgttgcaa aaatccggcg gtaaaaccgt cagcgaagaa
    43681 gaggtgctgc aactggtcca gttaagcaaa ccggaaatcg cgcaggcgtt atttggtacc
    43741 acgctggctg agttcagcca acgcagccgc gccgcctaca gtggacaaca gatgctggag
    43801 gagtatgtca acttctatca gaatctgtag ctacctgctg ctgccgctga tctacctgct
    43861 ggttaacgtc aaaatcaccc agcttggcga aagtttcccc atcaccatcg tcactttttt
    43921 acctgtcttg ttactgctgt ttttagaacg catcagcgtt aaaaaattga tgattgcctt
    43981 agggatttgc gcgggactca cggcgtttaa ctacctgttt ggtcagtcgc tggatgccag
    44041 taaatacgtc acttcaacta tgctgtttgt ctatatcgtg atcattattg gtatggtgtg
    44101 gagtattcgc tttaaaacga tttcgccaca caaccatcgc aagatattac gtttctttta
    44161 tctggtggtc gggctggtgg tggcgctggc ggcggtggag atggcgcaaa ttatcctcac
    44221 cggcggcagc agtattatgg agtcgatttc gaaatatctg atttacagca acagctatgt
    44281 gctgaatttc atcaaattcg gcggcaagcg cacgacagca ctttatttcg aaccggcatt
    44341 tttcgctctg gcattaatct caatttggct cagcatcaaa cagtttggta tcaaaacgcc
    44401 taaaacagat gctatgattc tcgcagggat aatattatcc ggatcgtttt caggggttat
    44461 gacctttatc ctgttttatt tgctggagtg ggcatttcaa tatctgaata aagaggcgat
    44521 taagaaaaag ttaccgttag cattgatttc tctggcagta ttcctggttg gtgtggtaat
    44581 tgcatttcct tatatttcca cccgtctggg cgatttaggt acggaaggat cgtcatcgta
    44641 ttatcgtatt gtcggtccgc tggtgatggt cggttattct ttgacccata ttgacggtgt
    44701 agtcagattt ggctcacttt atgaatatgt cgcatcattc ggaatattta acggtgcgga
    44761 tgtcggaaaa accatagaca atggtttgta tctgctgatt atttattttt cttggttcgc
    44821 ggtgttttta tcgctgtggt acatggggaa agtgataaaa atgatgatca acgcttttgg
    44881 tgataaccgc aattttcgcg tgcaattata tctttttact ccggtatcgc tgttttttac
    44941 cggttcgata tttagcccgg aatatgcatt tttaatcgtc tgtccgttta ttttgcgaaa
    45001 agcgttaaat attacgaggt aagaataaga acatgttgct tagcataatc actgtcgcgt
    45061 ttcgtaacct cgaagggata gtcaaaacac atgcctcgct ggcgcatctg gcgcaggcgg
    45121 aagatatcag cttcgaatgg attgttgtcg atggcggttc caatgacggc actcgtgagt
    45181 acctggaaaa tctcaatggt atctataacc tacgctttgt cagcgagcca gataacggta
    45241 tctacgacgc catgaacaag ggcattgcga tggcacaagg caagttcgcg ttgtttctca
    45301 actcgggcga tatttttcat caggatgccg catattttgt ccgtaagtta aaaatgcaaa
    45361 aagataacgt gatgatcacc ggcgatgcgc tgctggattt tggcgacggg cataaaatta
    45421 aacgtagcgc caaaccgggc tggtatattt atcacagcct gcccgccagt catcaggcga
    45481 tatttttccc ggtatccggt ttgaaaaaat ggcgttatga cctggaatat aaagtttctt
    45541 ctgattacgc gctggcagcc aaaatgtata aagcaggtta tgcatttaaa aaactcaatg
    45601 gcctggtgtc tgaattttct atgggtgggg tatctaccac caataatatg gaattgtgtg
    45661 ctgacgcgaa aaaagtccaa cggcaaatat tacatgtgcc tggcttttgg gttgaattat
    45721 cctggcattt acgccagcgt actacctcaa agacgaaagc cttatataac aaaagctgaa
    45781 tataagggaa aacgatgcag gatttaagcg gtttctcggt gccgaaaggg ttccggggcg
    45841 gcaacgctat taaagtgcaa ttgtggtggg cagtacaggc aacaatattt gcctggtcgc
    45901 cacaagtatt gtatcgctgg cgggcatttt tattacgttt attcggcgca aaaataggaa
    45961 aaaacgtagt tattcgtccg tcagtaaaaa ttacctatcc gtggaaatta accttaggtg
    46021 attacgtgtg ggtcggcgat gacgtcaatt tatataccct cggtgaaata accattggcg
    46081 cacattcggt gatatcgcaa aaaagttatt tatgcaccgg gagccacgac catgcaagtc
    46141 aacatttcac cattaacgcc acgcctattg tgattggcga gaaatgctgg ctggcaaccg
    46201 atgtttttgt tgccccaggc gtcacgattg gcgacggcac cgtcgtgggt gcgcgaagca
    46261 gtgtttttaa atcgcttccg gcaaatgtgg tttgccgggg gaatcccgca gtggtgatac
    46321 gcaaacgcgt tgaaactgaa taaattcaaa aaaaatacag aggaataaga catgtcaaaa
    46381 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
    46441 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
    46501 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
    46561 gacctgagtg atacctccaa cctgacgcgt attttgcgtg aagtgcagcc ggatgaagtg
    46621 tacaacctgg gcgcaatgag ccacgttgcg gtttcttttg agtcaccgga atataccgct
    46681 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
    46741 aagaaaaccc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
    46801 ccgcagaaag agaccacgcc gttctatccg cgctctccgt atgcggtcgc caaactgtat
    46861 gcctactgga tcaccgtcaa ctaccgtgaa tcctacggca tgtatgcctg taacggtatt
    46921 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
    46981 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
    47041 ctgcgtgact ggggccatgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
    47101 gaacagccag aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagttcgta
    47161 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg cgttgaagag
    47221 aagggcattg tggtttccgt caccgggcat gacgcgccgg gtgttaaacc gggtgatgtg
    47281 attatcgccg ttgatccgcg ttacttccgt ccggctgaag ttgaaacgct gctcggcgac
    47341 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
    47401 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
    47461 ggctacgacg tggcgatcgc gctggagtca taagtatgag tagacaacgc atttttattg
    47521 ctggccatcg cgggatggtc ggttccgcca tcaggcggca gctcgaacag cgcggtgatg
    47581 tggaactggt attacgcacc cgcgacgagc tgaacctgct ggacagccgc gccgtgcatg
    47641 atttctttgc cagcgaacgt attgaccagg tctatctggc ggccgcgaaa gtgggcggca
    47701 ttgttgccaa caacacgtat ccggcggatt tcatctatca gaacatgatg attgagagca
    47761 acatcattca cgccgcgcat cagaacgacg tgaacaaact gctgtttctc ggatcgtcct
    47821 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
    47881 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
    47941 catacaaccg ccagtacgga cgcgattacc gctcagtcat gccgaccaac ctgtatggcc
    48001 cgcatgacaa cttccacccg agtaattcgc atgtgatccc tgcattgctg cgtcgcttcc
    48061 acgaggcgac ggcacagaat gcaccggacg tggtggtgtg gggcagcggt acgccgatgc
    48121 gagaatttct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
    48181 atgaagtctg gctggagaac acccagccga tgctgtcgca cattaacgtc ggcacgggcg
    48241 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
    48301 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
    48361 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
    48421 accagtggtt ccttgagaat caagaccgct ttcgggggta atgatgtttt tacgtcagga
    48481 agactttgcc acggtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
    48541 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
    48601 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
    48661 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
    48721 gcacttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
    48781 ttttcgcttc agagtagcgg aagaagaact gttactgccg gatgagcagc atgacgatta
    48841 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgcgc
    48901 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
    48961 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
    49021 ctggcggcgc aaggtcatga ggtgcgggtt attaccgcac cgccttacta cccgcagtgg
    49081 caagtgggcg agaactattc cgcctggcgg tacaaacgag aagagggggc cgctacggtg
    49141 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgcct gttgcatctc
    49201 ggcagttttg ccgtcagcag ttttttcccg ttgatggcgc aacgtcgctg gaagccggat
    49261 cgcattatcg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaaa
    49321 ctctctggcg cgcgtaccgt gctgcatatt caggattacg aagtagatgc catgctgggg
    49381 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
    49441 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
    49501 gccatcgaaa aaggcgtggc ggcggaaaac gtcatcttct tccccaactg gtcggaaatc
    49561 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
    49621 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
    49681 aacgttattg aagcagccga tcgcctgcgc gatgaaccgc tgatttttgc cattgtcggg
    49741 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
    49801 caatttttcc cgctgcaatc gtatgacgct ttacccgcac tgctgaagat gggcgattgc
    49861 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
    49921 attctggcag taggcggtaa cgcggtgatt actgctgaag cccacacaga actgggacag
    49981 ctttgcgaaa cctttccggg cattgcggtt tgcgtagaac cggaatcggt tgaggcgctg
    50041 gtggcgggga ttcgtcaggc gctcctgctg cccaaacaca acacggtggc acgtgaatat
    50101 gccgaacgca cgcttgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
    50161 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgct
    50221 tatggccgct ttcccgcgta ctttatccca agcagttttt atgcctgaaa ggcgatctca
    50281 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc cctgtggtga
    50341 tttgcaatga gcagcaccgc tttattgttg cggaacaact gcgtcaactg aacaaactca
    50401 ctgagaacat tattctcgaa ccggcagggc gtaacacagc accggccatt gcgctggcgg
    50461 cgctggcggc aaaacgtcat agcccggaga acgacccatt aatgctggtg ctggcggcgg
    50521 atcatgtgat tgccgatgaa gacgcattcc gcgccgccgt gcgtaacgct atgccgtatg
    50581 ccgaagcggg caagctggtg accttcggca ttgtgccgga tctacctgaa accggatatg
    50641 gctatattcg tcgcggtgaa gtgtcggcgg gtgagcagga tacggtggcc tttgaagtgg
    50701 cgcagtttgt cgaaaaacca aatctggaaa ccgcgcaggc ctatgtggca agcggcgaat
    50761 attactggaa cagcggtatg ttcctgttcc gtgccggacg ctatctcgaa gaactgaaaa
    50821 aatatcgccc ggatattctc gacgcctgtg aaaaagcgat gagcgccgtc gatccggatc
    50881 tcgattttat tcgcgtggat gaagacgcgt ttctggcctg cccggaagag tcggtggatt
    50941 acgcggtcat ggaacgtacg gcagatgccg ttgtggtgcc gatggatgcg ggctggagcg
    51001 atgtcggctc ctggtcctca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
    51061 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatctggcc
    51121 tggtcaccac cgtcggggtg aaagatttgg tggtagtgca gaccaaagat gcggtgctga
    51181 ttgccgaccg taacgcggtg caggatgtga aaaaagtggt cgagcagatc aaagccgatg
    51241 gtcgccatga gcatcgggtg catcgcgaag tctatcgccc gtggggcaaa tatgactcta
    51301 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gaaggcttgt
    51361 cggtacagat gcaccatcac cgcgcggaac actgggtggt tgtcgcggga acggcaaaag
    51421 tcaccattga cggtgatatc aaactgcttg gtgaaaacga gtcgatttat attccgctgg
    51481 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
    51541 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
    51601 aaacgtcgca tcaggcgctg attgtcggat gcggcgtgaa cgccttatcc gacctacggt
    51661 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
    51721 cggtgtaaat aacgacaaaa acaaaattgg ccgtttcggt cagggccaac tattgcctga
    51781 aaaagggtaa cgatatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
    51841 taggcgaaga actgaatgaa gatattgcct ggcgcattgg tcgtgcctat ggcgaatttc
    51901 tcaaaccgaa aaccattgtg ttaggcggtg atgcccgtct caccagcgaa accttaaaac
    51961 tggcgctggc aaaaggttta caggatgcgg gcgttgacgt gctggatatt ggtatgtccg
    52021 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
    52081 ctgccagcca taacccgatg gattacaacg gcatgaaact ggtgcgcgaa ggggctcgcc
    52141 cgatcagcgg cgataccgga ctgcgcgatg tccagcgtct ggcagaagcc aacgactttc
    52201 ctcccgttga tgaaaccaaa cgcggtcgct atcagcaaat caatctgcgt gacgcttacg
    52261 ttgatcacct gttcggttat atcaacgtca aaaaccttac gccgctcaag ctggtgatca
    52321 actccgggaa cggcgcggcg ggtccggtgg tggacgccat cgaagcccgc tttaaagccc
    52381 tcggcgcacc ggtggaattg atcaaagtgc acaacacgcc cgacggcaat ttccccaacg
    52441 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcattaaac
    52501 acggcgcgga tatgggcatt gcttttgatg gcgattttga ccgctgtttc ctgtttgacg
    52561 aaaaagggca gtttatcgag ggctactaca ttgtcggtct gctggcagaa gcgttcctcg
    52621 aaaaaaatcc cggcgcgaag atcatccacg atccacgtct ctcctggaat accgttgatg
    52681 tggtgactgc cgcaggcggc accccggtaa tgtcgaaaac cggacacgcc tttattaaag
    52741 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
    52801 gtgattttgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
    52861 gcctgaaagg aaagacgctg ggcgaactgg tgcgcgaccg gatggcagcg tttccgtcaa
    52921 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
    52981 attttagccg tgaggcgctg gcggtggatc gcaccgatgg catcagcatg acctttgccg
    53041 actggcgctt taatctgcgc tcctccaaca ccgaaccggt ggtgcggttg aatgtggaat
    53101 cacgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
    53161 agtaatgtcg gatcttccct taccccattg cgggtaaggg gctaataaca ggaacaacga
    53221 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
    53281 aacgcttttc agatatcacc atcatgtttg ccggactatg gctggtttgc gaagtgagcg
    53341 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
    53401 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
    53461 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
    53521 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagta
    53581 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
    53641 gctataacaa gcgcatggtc gcggtggcgg gggatttagc cgccgggcaa atgctgatgg
    53701 agagcttccg taaccagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
    53761 aactgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcta
    53821 aagcaggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcgcgagtga
    53881 aaaaactggt ccatcaactg gcggacacca cctgttcggt gctgctgatc cccgatgtct
    53941 ttaccttcaa cattctccat tcacgtcttg aagagatgaa cggcgttccg gtggtgccgc
    54001 tttacgacac gccgctttcc ggggttaacc gcctgctcaa acgtgcggaa gacattgtgc
    54061 tggcgacgct tatcctgctg ctgatctccc cggtgctgtg ctgcattgcg ctggcggtga
    54121 aactcagttc acctgggccg gttattttcc gccagactcg ctacggcatg gatggcaagc
    54181 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
    54241 aggcgacgca gaacgatccg cgcgtcacca aagtggggaa ctttctgcgc cgcacctcgc
    54301 tggatgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
    54361 gtccgcacgc ggtggcgcat aacgaacagt atcgacagct cattgaaggc tacatgctgc
    54421 gtcataaggt gaaaccgggc attaccggct gggcgcagat taacggctgg cgcggcgaaa
    54481 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
    54541 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaaagga ttcgttaaca
    54601 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggcg
    54661 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
    54721 atcgacaacc accagttcgg tttgcttacc gtgtcgctgg tgatcatcgc gctggcagat
    54781 acgctttctg acttcggtat tgctaactcg attattcagc gaaaagaaat cagccacctt
    54841 gaactcacca cgctgtactg gctgaacgtt gggctggggc ttgtggtgtg tgtggcggtg
    54901 tttttgttga gtgatctcat tggtgacgtg ctgaacaacc cggacctggc accgttgatt
    54961 aaaacattgt cgctggcgtt tgtggttatc ccccacgggc aacagttccg cgcgttgatg
    55021 caaaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt actggcgggc
    55081 ttcactttta cggtggttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
    55141 tatctggtca atagtgcggt gagaacgctg ctgtttggct actttggccg caaaatttat
    55201 cgccccggtc tgcatttctc gctggcgtct gttgcgccga acttacgctt tggtgcctgg
    55261 ctgacggcgg acagcatcat caactatctc aataccaacc tttcaacgct ggtgctggcg
    55321 cgtattctcg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgttgtg
    55381 ccgccgatga agctgaaccc gatcatcacc cgtgtgttgt ttccggcatt cgccaaaatt
    55441 caggacgata ccgaaaagct gcgcgtcaac ttctacaagc tgctgtcggt ggtggggatt
    55501 atcaactttc cggcgctgct cgggctgatg gtggtgtcaa ataactttgt accgctggta
    55561 tttggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtgt ggtgggtctg
    55621 ctgcgctccg taggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    55681 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    55741 cagattgcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    55801 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    55861 ctgagtttgt ggctgccgtt ttatctctcg ctgccgacgc tggtggtcag ttatgtgctg
    55921 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    55981 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcgcgcc atccgctggt ggtggaagtg
    56041 aagcgccagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgagttgtt
    56101 gttttcggtt ccgacaacgg ttgccgggtg cagcgtaaaa caccttatcc atcctacggt
    56161 tctgtgctcg ggtcttgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    56221 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    56281 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    56341 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    56401 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg tgatccgctg ttccttcaaa
    56461 tgaaacaaca caacagcgcg gcgggcgttg tcgggcgcgt taaaaaagtc ctccgtcgcc
    56521 gctatcagca ccaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    56581 ccatcgccca gggattcacc gacttcgtgc gcctactgtc aggttacgac gccattattc
    56641 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcacttt
    56701 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggt cccttccagg
    56761 atgagcaatt taaccaactg gcgaactacg tttttggtca ctgcgacgcg ctgatcttgc
    56821 gcgaatcggt cagcctcgat ctaatgaaac gcagcaatat caccactgca aaagttgaac
    56881 atggcgtcga taccgcgtgg ctggtcgatc accacacaga agacttcacc gccagctatg
    56941 ccgtccaaca ctggctggac gttgccgcac aacagaaaac ggtggcaatt accctgcgcg
    57001 aactggcacc gttcgacaaa cgcctcggca ccactcagca agtgtatgaa aaagcctttg
    57061 ccggggtggt caatcgcatt ctcgacgaag gctatcaggt cattgcgctc tccacctgta
    57121 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaacctg cgccagcaca
    57181 tcagcgatcc tgcccgttat cacgtagtga tggatgaact taacgatctg gaaatgggca
    57241 aaattctcgg tgcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    57301 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc ggggttatgc
    57361 agcagctggg actaccggag atggcaattg atatccgtca tttattagac ggcagcctgc
    57421 aagcgatggt tgcggatacc ttaggccagc ttccggcgct gaacgcacga cttagcgaag
    57481 ccgtcagtcg tgagcgtcag acgggaatgc agatggtgca gtccgtactg gaacgcatcg
    57541 gggaggtgaa atgaaggtcg gcttcttctt attgaaattt ccgctgtcgt cagaaacctt
    57601 cgttcttaac caaatcactg cgtttattga tatgggcttt gaagtggaga ttgtcgcgct
    57661 gcaaaaaggc gacacacaaa acacccacgt ggcatggact aaatacaacc ttgccgccag
    57721 aacccgctgg ttacaggacg aacctacggg caaagtggcg aaactgagcc accgagccag
    57781 tcagaccttg cgcggcattc atcgtaaaaa tacctggcag gcgctcaacc tcaaacgcta
    57841 tggtgccgag tcgtggaacc tgattttgtc tgccatttgc ggccaggtcg caacaccgtt
    57901 tcgcgccgat gtgttcatcg ctcattttgg ccctgcgggg gtaaccgcag caaaactacg
    57961 cgaactgggt gtcattcacg gcaaaattgc cactattttc cacggcattg atatttccag
    58021 tcgggaagtg ctcaaccatt acactcccga atatcaacaa ctgtttcgac gtggcgacct
    58081 gatgctaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gcccgaggga
    58141 aaaaatcgcc gtatcgcgca tgggcgtaga tatgacgcgc tttagcccgc gtcccgtgaa
    58201 agcgcccgca acaccgctgg agattatttc cgtcgcacgc ttaaccgaga aaaaaggtct
    58261 gcatgtggcg attgaagcct gccggcagtt gaaagagcag ggcgtggcat ttcgctatcg
    58321 catcctcggc attggcccgt gggaacgacg cctgcgcacg ctcatcgaac aatatcaact
    58381 ggaagatgtg gtggagatgc cgggctttaa accgagccat gaagtgaaag cgatgctcga
    58441 cgacgcggat gtcttcctgt tgccatcggt tacaggtgcg gatggcgata tggaaggcat
    58501 tccggtggcg ctgatggagg cgatggcggt cggcattccg gtggtttcta ctctgcatag
    58561 cggaataccg gaactggtgg aggctgacaa atccggctgg ctggtgcctg agaacgatgc
    58621 tcgcgcactg gcgcaacgac tggcggcgtt tagccaactg gacaccgacg aattggctcc
    58681 ggtcgtcaaa cgcgcgcgcg aaaaagttga acacgatttt aaccagcagg tgatcaatcg
    58741 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaaaactct
    58801 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt ccttcatacc cctttcgctc
    58861 gcgcatttcc cgcccgacaa agcgttaata tcaacgacta caaccctcac gactggatcg
    58921 cctcatttaa acaagccttc agcgaagggc aaacggttgt cgtgcctgct ggattcgttt
    58981 gtgacaatat caacaccggc atcttcattc ctcctggcaa aacgttacac atccttggaa
    59041 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    59101 aggagggcgg cagtatgcat aacatcaccc tggatgtgcg tggttctgac tgcaccatca
    59161 aagggctggc gatgagcggc tttggtccgg taacgcagat ttatatcggc ggcaaaaaca
    59221 aacgggtcat gcgcaacctg accatcgata acctgaccat tagccacgct aattacgcca
    59281 tcttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaat tgtaagttca
    59341 gcgacttaca gggcgatgcc attgaatgga acgtggcgat taacgaccgc gatattttga
    59401 tctccgacca tgtcatcgag cgcatcaact gtactaacgg caaaatcaac tggggcattg
    59461 gcataggcct tgcgggaagc acttacgata ataattaccc ggaagatcag gcagtgaaaa
    59521 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatacat gttgaaaatg
    59581 gtaaacattt tgttattcgt aatatcaaag cccgcaatat cacgcaggat ttcagtaaga
    59641 aagcaggtat tgataacgcc acagtcgcta tttacggttg tgacaatttc gtgattgata
    59701 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaatt
    59761 atctctcgat tccgcaaaat ttccgagtga ataatattca actggataac acccatcttg
    59821 cttataaatt gcgcggcctc caaatctctg ccgggaatgc cgtctccttt gtggcactaa
    59881 ctaacattga gatgaagcgt gcgtcgctgg agttacacaa caaaccgcaa catctttttc
    59941 tgcgtaatat caaagtgatg caggaatcct ctgttggacc cgcattgatt atgaacttcg
    60001 acatgcgcaa agacgttcga ggcgtcttta tggcgaaaga agaaacactg ctgtctcttg
    60061 caaatgttca tgcggtgaat gagaaaggac aaagctccgt cgatatcgac aggattaatc
    60121 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggaga gagtagattt
    60181 gcgaccattc ataaacttac tggatataat gttctaaaag attataatcc tcacactaac
    60241 tgttaaagct ggggctgggc agcattgact actcagggaa ggtactcatc tttatcttgt
    60301 gtttcttacg taacaaccag actaaagtga tttatgaacg ataacgtttt gctcatagga
    60361 gcttccggat tcgtaggaac ccgactactt gaaacggcaa ttgctgactt taatatcaag
    60421 aacctggaca aacagcagag ccacttttat ccagaaatca cacagattgg tgatgttcgt
    60481 gatcaacagg cactcgacca ggcgttagcc ggttttgaca ctgttgtact actggcagcg
    60541 gaacaccgcg atgacgtcag ccctacttct ctctattatg atgtcaacgt tcagggtacc
    60601 cgcaatgtgc tggcggccat ggaaaaaaat ggcgttaaaa atatcatctt taccagttcc
    60661 gttgctgttt atggtttgaa caaacacaac cctgacgaaa accatccaca cgaccctttc
    60721 aaccactacg gcaaaagcaa gtggcaggcg gaggaagtgc tgcgtgaatg gtataacaaa
    60781 gcaccaacag aacgttcatt aactatcatc cgtcctaccg ttatcttcgg tgaacgcaac
    60841 cgcggtaacg tctataactt gctgaaacag atcgctggcg gcaagtttat gatggtgggc
    60901 gcagggacta actataagtc catggcttat gttggaaaca ttgttgagtt tatcaagtac
    60961 aaactgaaga atgttgccgc aggttacgag gtttataact acgttgataa gccagacctg
    61021 aacatgaacc agttggttgc tgaagttgaa caaagcctga acaaaaagat cccttctatg
    61081 cacttgcctt acccactagg aatgctgggt ggatattgct ttgatatcct gagcaaaatt
    61141 acgggcaaaa aatacgctgt cagctctgtg cgcgtgaaaa aattctgcgc aacaacacag
    61201 tttgacgcaa cgaaagtgca ttcttcaggt tttgtggcac cgtatacgct gtcgcaaggt
    61261 ctggatcgaa ctctgcagta tgaattcgtc catgccaaaa aagacgacat aacgtttgtt
    61321 tctgagtaat gcagacgagt cagagaaaaa aggaccaggt tggactacga ttattcggaa
    61381 agaggtactg attaaattat cagtatgtgg atttccgagt aatccgagta acccagatgc
    61441 tgatggtgtg tagtgttatc gtaaggttta atcaaagtta tgcggaacta agctttggta
    61501 tatagccata acgtttggct aacctatggc aataatttac gcactatcag ccacttagag
    61561 gtaggaaaag aatgacgaat ttaaaagcag ttataccggt agcaggtctt gggatgcata
    61621 tgttgcctgc cactaaggcg attcccaaag agatgctacc gatcgtcgac aagccaatga
    61681 ttcagtacat tgttgacgag attgtggctg cagggatcaa agaaatcctc ctggtaactc
    61741 acgcgtccaa gaacgcagtc gaaaaccact tcgacacctc ttatgaatta gaatctctcc
    61801 ttgaacagcg cgtgaagcgt caactactgg cggaagtaca atctatctgt ccgccgggcg
    61861 tgaccattat gaacgtgcgt cagggtgaac ctttaggttt aggccactcc attttatgtg
    61921 cgcgacctgc cattggtgac aatccatttg tcgtggtgct gccagacgtt gtgatcgacg
    61981 acgccagcgc cgacccgcta cgctacaacc ttgctgccat gattgcgcgc ttcaatgaaa
    62041 cgggacgcag ccaggtgctg gcaaaacgta tgccgggcga cctctctgaa tactccgtca
    62101 tccagaccaa agaaccgctg gatcgtgaag gcaaagtcag ccgcattgtt gaatttatcg
    62161 aaaagccgga tcagccgcag acgctggact cagacatcat ggccgttggt cgatatgtgc
    62221 tttctgccga tatttggccg gaactggaac gtactcagcc tggtgcatgg ggacgtattc
    62281 agctgactga tgccattgct gagctggcaa aaaaacaatc cgttgatgca atgctgatga
    62341 ctggcgacag ttacgactgc ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg
    62401 gactacgcaa cttgaaagaa ggggcgaagt tccgcaaagg tatcgagaag ctgttaagcg
    62461 aataatgaaa atctgaccga atgtaacggt tgataaggaa ataatatcgg cagtgaaaaa
    62521 ctatagatca ataaatatca ctgctgttga gcttttataa ttaactatgc taccaatgaa
    62581 taaatagtgt gcagatatgt attttttgtt tggtagtctt gtttctccct ctgaataata
    62641 agataataaa cgcttggaga tgattttttt aacatggatt atcatttcgt acactggtag
    62701 cgattaagcc aggggcggta gcgtgcataa attttaatgc ttatcaaaac tattagcatt
    62761 aaaaatatat aagaaattct caaatgaaca aagaaaccgt ttcaataatt atgcccgttt
    62821 acaatggggc caaaactata atctcatcag tagaatcaat tatacatcaa tcttatcaag
    62881 attttgtttt gtatatcatt gacgattgta gcaccgatga tacattttca ttaatcaaca
    62941 gtcgatacaa aaacaatcag aaaataagaa tattgcgtaa caagacaaat ttaggtgttg
    63001 cagaaagtcg aaattatgga atagaaatgg ccacggggaa atatatttct ttttgtgatg
    63061 cggatgattt gtggcacgag aaaaaattag agcgtcaaat cgaagtgtta aataatgaat
    63121 gtgtagatgt ggtatgttct aattattatg ttatagataa caaaagaaat attgttggcg
    63181 aagttaatgc tcctcatgtg ataaattata gaaaaatgct catgaaaaac tacataggga
    63241 atttgacagg aatctataat gccaacaaat tgggtaagtt ttatcaaaaa aagattggtc
    63301 acgaggatta tttgatgtgg ctggaaataa ttaataaaac aaatggtgct at
//
LOCUS       DABSYT020000017        62862 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.017, whole genome shotgun sequence.
ACCESSION   DABSYT020000017 DABSYT020000000
VERSION     DABSYT020000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 62862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.017"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            209..1582
                     /gene="aroP"
                     /locus_tag="ID725_RS07200"
                     /old_locus_tag="pgaptmp_001440"
     CDS             209..1582
                     /gene="aroP"
                     /locus_tag="ID725_RS07200"
                     /old_locus_tag="pgaptmp_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGV7850076.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1625..2479)
                     /gene="ampE"
                     /locus_tag="ID725_RS07205"
                     /old_locus_tag="pgaptmp_001441"
     CDS             complement(1625..2479)
                     /gene="ampE"
                     /locus_tag="ID725_RS07205"
                     /old_locus_tag="pgaptmp_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGV7850077.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2476..3027)
                     /gene="ampD"
                     /locus_tag="ID725_RS07210"
                     /old_locus_tag="pgaptmp_001442"
     CDS             complement(2476..3027)
                     /gene="ampD"
                     /locus_tag="ID725_RS07210"
                     /old_locus_tag="pgaptmp_001442"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGV7850078.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3115..4008
                     /gene="nadC"
                     /locus_tag="ID725_RS07215"
                     /old_locus_tag="pgaptmp_001443"
     CDS             3115..4008
                     /gene="nadC"
                     /locus_tag="ID725_RS07215"
                     /old_locus_tag="pgaptmp_001443"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGV7850079.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4211..4651
                     /gene="ppdD"
                     /locus_tag="ID725_RS07220"
                     /old_locus_tag="pgaptmp_001444"
     CDS             4211..4651
                     /gene="ppdD"
                     /locus_tag="ID725_RS07220"
                     /old_locus_tag="pgaptmp_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGV7850080.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4661..6046
                     /gene="gspE"
                     /locus_tag="ID725_RS07225"
                     /old_locus_tag="pgaptmp_001445"
     CDS             4661..6046
                     /gene="gspE"
                     /locus_tag="ID725_RS07225"
                     /old_locus_tag="pgaptmp_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGV7850081.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6036..7238
                     /gene="hofC"
                     /locus_tag="ID725_RS07230"
                     /old_locus_tag="pgaptmp_001446"
     CDS             6036..7238
                     /gene="hofC"
                     /locus_tag="ID725_RS07230"
                     /old_locus_tag="pgaptmp_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGV7850082.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7273..8316)
                     /gene="guaC"
                     /locus_tag="ID725_RS07235"
                     /old_locus_tag="pgaptmp_001447"
     CDS             complement(7273..8316)
                     /gene="guaC"
                     /locus_tag="ID725_RS07235"
                     /old_locus_tag="pgaptmp_001447"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGV7850083.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8472..8516)
                     /locus_tag="ID725_RS07240"
                     /old_locus_tag="pgaptmp_001448"
     CDS             complement(8472..8516)
                     /locus_tag="ID725_RS07240"
                     /old_locus_tag="pgaptmp_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850084.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8541..9161
                     /gene="coaE"
                     /locus_tag="ID725_RS07245"
                     /old_locus_tag="pgaptmp_001449"
     CDS             8541..9161
                     /gene="coaE"
                     /locus_tag="ID725_RS07245"
                     /old_locus_tag="pgaptmp_001449"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGV7850085.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9161..9904
                     /gene="zapD"
                     /locus_tag="ID725_RS07250"
                     /old_locus_tag="pgaptmp_001450"
     CDS             9161..9904
                     /gene="zapD"
                     /locus_tag="ID725_RS07250"
                     /old_locus_tag="pgaptmp_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGV7850086.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9914..10111
                     /gene="yacG"
                     /locus_tag="ID725_RS07255"
                     /old_locus_tag="pgaptmp_001451"
     CDS             9914..10111
                     /gene="yacG"
                     /locus_tag="ID725_RS07255"
                     /old_locus_tag="pgaptmp_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGV7850087.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10202..10600)
                     /gene="mutT"
                     /locus_tag="ID725_RS07260"
                     /old_locus_tag="pgaptmp_001452"
     CDS             complement(10202..10600)
                     /gene="mutT"
                     /locus_tag="ID725_RS07260"
                     /old_locus_tag="pgaptmp_001452"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGV7850088.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10660..13365)
                     /gene="secA"
                     /locus_tag="ID725_RS07265"
                     /old_locus_tag="pgaptmp_001453"
     CDS             complement(10660..13365)
                     /gene="secA"
                     /locus_tag="ID725_RS07265"
                     /old_locus_tag="pgaptmp_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGV7850089.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13427..13939)
                     /gene="secM"
                     /locus_tag="ID725_RS07270"
                     /old_locus_tag="pgaptmp_001454"
     CDS             complement(13427..13939)
                     /gene="secM"
                     /locus_tag="ID725_RS07270"
                     /old_locus_tag="pgaptmp_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGV7850090.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14170..15087)
                     /gene="lpxC"
                     /locus_tag="ID725_RS07275"
                     /old_locus_tag="pgaptmp_001455"
     CDS             complement(14170..15087)
                     /gene="lpxC"
                     /locus_tag="ID725_RS07275"
                     /old_locus_tag="pgaptmp_001455"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGV7850091.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15188..16339)
                     /gene="ftsZ"
                     /locus_tag="ID725_RS07280"
                     /old_locus_tag="pgaptmp_001456"
     CDS             complement(15188..16339)
                     /gene="ftsZ"
                     /locus_tag="ID725_RS07280"
                     /old_locus_tag="pgaptmp_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGV7850092.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16400..17662)
                     /gene="ftsA"
                     /locus_tag="ID725_RS07285"
                     /old_locus_tag="pgaptmp_001457"
     CDS             complement(16400..17662)
                     /gene="ftsA"
                     /locus_tag="ID725_RS07285"
                     /old_locus_tag="pgaptmp_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGV7850093.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17659..18489)
                     /gene="ftsQ"
                     /locus_tag="ID725_RS07290"
                     /old_locus_tag="pgaptmp_001458"
     CDS             complement(17659..18489)
                     /gene="ftsQ"
                     /locus_tag="ID725_RS07290"
                     /old_locus_tag="pgaptmp_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGV7850094.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18491..19411)
                     /gene="ddlB"
                     /locus_tag="ID725_RS07295"
                     /old_locus_tag="pgaptmp_001459"
     CDS             complement(18491..19411)
                     /gene="ddlB"
                     /locus_tag="ID725_RS07295"
                     /old_locus_tag="pgaptmp_001459"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGV7850095.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19404..20879)
                     /gene="murC"
                     /locus_tag="ID725_RS07300"
                     /old_locus_tag="pgaptmp_001460"
     CDS             complement(19404..20879)
                     /gene="murC"
                     /locus_tag="ID725_RS07300"
                     /old_locus_tag="pgaptmp_001460"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGV7850096.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20933..22000)
                     /gene="murG"
                     /locus_tag="ID725_RS07305"
                     /old_locus_tag="pgaptmp_001461"
     CDS             complement(20933..22000)
                     /gene="murG"
                     /locus_tag="ID725_RS07305"
                     /old_locus_tag="pgaptmp_001461"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGV7850097.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21997..23241)
                     /gene="ftsW"
                     /locus_tag="ID725_RS07310"
                     /old_locus_tag="pgaptmp_001462"
     CDS             complement(21997..23241)
                     /gene="ftsW"
                     /locus_tag="ID725_RS07310"
                     /old_locus_tag="pgaptmp_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGV7850098.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23241..24557)
                     /gene="murD"
                     /locus_tag="ID725_RS07315"
                     /old_locus_tag="pgaptmp_001463"
     CDS             complement(23241..24557)
                     /gene="murD"
                     /locus_tag="ID725_RS07315"
                     /old_locus_tag="pgaptmp_001463"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGV7850099.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24560..25642)
                     /gene="mraY"
                     /locus_tag="ID725_RS07320"
                     /old_locus_tag="pgaptmp_001464"
     CDS             complement(24560..25642)
                     /gene="mraY"
                     /locus_tag="ID725_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGV7850100.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25636..26994)
                     /gene="murF"
                     /locus_tag="ID725_RS07325"
                     /old_locus_tag="pgaptmp_001465"
     CDS             complement(25636..26994)
                     /gene="murF"
                     /locus_tag="ID725_RS07325"
                     /old_locus_tag="pgaptmp_001465"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGV7850101.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26991..28478)
                     /gene="murE"
                     /locus_tag="ID725_RS07330"
                     /old_locus_tag="pgaptmp_001466"
     CDS             complement(26991..28478)
                     /gene="murE"
                     /locus_tag="ID725_RS07330"
                     /old_locus_tag="pgaptmp_001466"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGV7850102.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28465..30231)
                     /gene="ftsI"
                     /locus_tag="ID725_RS07335"
                     /old_locus_tag="pgaptmp_001467"
     CDS             complement(28465..30231)
                     /gene="ftsI"
                     /locus_tag="ID725_RS07335"
                     /old_locus_tag="pgaptmp_001467"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGV7850103.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30247..30612)
                     /gene="ftsL"
                     /locus_tag="ID725_RS07340"
                     /old_locus_tag="pgaptmp_001468"
     CDS             complement(30247..30612)
                     /gene="ftsL"
                     /locus_tag="ID725_RS07340"
                     /old_locus_tag="pgaptmp_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGV7850104.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30609..31550)
                     /gene="rsmH"
                     /locus_tag="ID725_RS07345"
                     /old_locus_tag="pgaptmp_001469"
     CDS             complement(30609..31550)
                     /gene="rsmH"
                     /locus_tag="ID725_RS07345"
                     /old_locus_tag="pgaptmp_001469"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGV7850105.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31552..32010)
                     /gene="mraZ"
                     /locus_tag="ID725_RS07350"
                     /old_locus_tag="pgaptmp_001470"
     CDS             complement(31552..32010)
                     /gene="mraZ"
                     /locus_tag="ID725_RS07350"
                     /old_locus_tag="pgaptmp_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGV7850106.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32612..33616)
                     /gene="cra"
                     /locus_tag="ID725_RS07355"
                     /old_locus_tag="pgaptmp_001471"
     CDS             complement(32612..33616)
                     /gene="cra"
                     /locus_tag="ID725_RS07355"
                     /old_locus_tag="pgaptmp_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGV7850107.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33698..33784)
                     /locus_tag="ID725_RS07360"
                     /old_locus_tag="pgaptmp_001472"
     CDS             complement(33698..33784)
                     /locus_tag="ID725_RS07360"
                     /old_locus_tag="pgaptmp_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGV7850108.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33796..34287)
                     /gene="ilvN"
                     /locus_tag="ID725_RS07365"
                     /old_locus_tag="pgaptmp_001473"
     CDS             complement(33796..34287)
                     /gene="ilvN"
                     /locus_tag="ID725_RS07365"
                     /old_locus_tag="pgaptmp_001473"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGV7850109.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34290..36014)
                     /gene="ilvI"
                     /locus_tag="ID725_RS07370"
                     /old_locus_tag="pgaptmp_001474"
     CDS             complement(34290..36014)
                     /gene="ilvI"
                     /locus_tag="ID725_RS07370"
                     /old_locus_tag="pgaptmp_001474"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGV7850110.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36332..37276)
                     /gene="leuO"
                     /locus_tag="ID725_RS07375"
                     /old_locus_tag="pgaptmp_001475"
     CDS             complement(36332..37276)
                     /gene="leuO"
                     /locus_tag="ID725_RS07375"
                     /old_locus_tag="pgaptmp_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGV7850111.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            37934..38020
                     /gene="leuL"
                     /locus_tag="ID725_RS07380"
                     /old_locus_tag="pgaptmp_001476"
     CDS             37934..38020
                     /gene="leuL"
                     /locus_tag="ID725_RS07380"
                     /old_locus_tag="pgaptmp_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGV7850112.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            38113..39684
                     /gene="leuA"
                     /locus_tag="ID725_RS07385"
                     /old_locus_tag="pgaptmp_001477"
     CDS             38113..39684
                     /gene="leuA"
                     /locus_tag="ID725_RS07385"
                     /old_locus_tag="pgaptmp_001477"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGV7850113.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            39684..40775
                     /gene="leuB"
                     /locus_tag="ID725_RS07390"
                     /old_locus_tag="pgaptmp_001478"
     CDS             39684..40775
                     /gene="leuB"
                     /locus_tag="ID725_RS07390"
                     /old_locus_tag="pgaptmp_001478"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGV7850114.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            40778..42178
                     /gene="leuC"
                     /locus_tag="ID725_RS07395"
                     /old_locus_tag="pgaptmp_001479"
     CDS             40778..42178
                     /gene="leuC"
                     /locus_tag="ID725_RS07395"
                     /old_locus_tag="pgaptmp_001479"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGV7850115.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            42189..42794
                     /gene="leuD"
                     /locus_tag="ID725_RS07400"
                     /old_locus_tag="pgaptmp_001480"
     CDS             42189..42794
                     /gene="leuD"
                     /locus_tag="ID725_RS07400"
                     /old_locus_tag="pgaptmp_001480"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGV7850116.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(42902..43033)
                     /gene="sgrT"
                     /locus_tag="ID725_RS07405"
                     /old_locus_tag="pgaptmp_001481"
     CDS             complement(42902..43033)
                     /gene="sgrT"
                     /locus_tag="ID725_RS07405"
                     /old_locus_tag="pgaptmp_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGV7850117.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            43122..44780
                     /gene="sgrR"
                     /locus_tag="ID725_RS07410"
                     /old_locus_tag="pgaptmp_001482"
     CDS             43122..44780
                     /gene="sgrR"
                     /locus_tag="ID725_RS07410"
                     /old_locus_tag="pgaptmp_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGV7850118.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(45009..45578)
                     /gene="espY2"
                     /locus_tag="ID725_RS07415"
                     /old_locus_tag="pgaptmp_001483"
     CDS             complement(45009..45578)
                     /gene="espY2"
                     /locus_tag="ID725_RS07415"
                     /old_locus_tag="pgaptmp_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HGV7850119.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      45820..45919
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            45950..46933
                     /gene="thiB"
                     /locus_tag="ID725_RS07420"
                     /old_locus_tag="pgaptmp_001484"
     CDS             45950..46933
                     /gene="thiB"
                     /locus_tag="ID725_RS07420"
                     /old_locus_tag="pgaptmp_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGV7850120.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            46909..48519
                     /gene="thiP"
                     /locus_tag="ID725_RS07425"
                     /old_locus_tag="pgaptmp_001485"
     CDS             46909..48519
                     /gene="thiP"
                     /locus_tag="ID725_RS07425"
                     /old_locus_tag="pgaptmp_001485"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGV7850121.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            48503..49201
                     /gene="thiQ"
                     /locus_tag="ID725_RS07430"
                     /old_locus_tag="pgaptmp_001486"
     CDS             48503..49201
                     /gene="thiQ"
                     /locus_tag="ID725_RS07430"
                     /old_locus_tag="pgaptmp_001486"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGV7850122.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(49288..50052)
                     /gene="yabI"
                     /locus_tag="ID725_RS07435"
                     /old_locus_tag="pgaptmp_001487"
     CDS             complement(49288..50052)
                     /gene="yabI"
                     /locus_tag="ID725_RS07435"
                     /old_locus_tag="pgaptmp_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGV7850123.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(50138..51016)
                     /gene="araC"
                     /locus_tag="ID725_RS07440"
                     /old_locus_tag="pgaptmp_001488"
     CDS             complement(50138..51016)
                     /gene="araC"
                     /locus_tag="ID725_RS07440"
                     /old_locus_tag="pgaptmp_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGV7850124.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            51355..53055
                     /gene="araB"
                     /locus_tag="ID725_RS07445"
                     /old_locus_tag="pgaptmp_001489"
     CDS             51355..53055
                     /gene="araB"
                     /locus_tag="ID725_RS07445"
                     /old_locus_tag="pgaptmp_001489"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGV7850125.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            53066..54568
                     /gene="araA"
                     /locus_tag="ID725_RS07450"
                     /old_locus_tag="pgaptmp_001490"
     CDS             53066..54568
                     /gene="araA"
                     /locus_tag="ID725_RS07450"
                     /old_locus_tag="pgaptmp_001490"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGV7850126.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            54768..55463
                     /gene="araD"
                     /locus_tag="ID725_RS07455"
                     /old_locus_tag="pgaptmp_001491"
     CDS             54768..55463
                     /gene="araD"
                     /locus_tag="ID725_RS07455"
                     /old_locus_tag="pgaptmp_001491"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGV7850127.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            55538..57889
                     /gene="polB"
                     /locus_tag="ID725_RS07460"
                     /old_locus_tag="pgaptmp_001492"
     CDS             55538..57889
                     /gene="polB"
                     /locus_tag="ID725_RS07460"
                     /old_locus_tag="pgaptmp_001492"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGV7850128.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            58053..60959
                     /gene="rapA"
                     /locus_tag="ID725_RS07465"
                     /old_locus_tag="pgaptmp_001493"
     CDS             58053..60959
                     /gene="rapA"
                     /locus_tag="ID725_RS07465"
                     /old_locus_tag="pgaptmp_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGV7850129.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            60971..61630
                     /gene="rluA"
                     /locus_tag="ID725_RS07470"
                     /old_locus_tag="pgaptmp_001494"
     CDS             60971..61630
                     /gene="rluA"
                     /locus_tag="ID725_RS07470"
                     /old_locus_tag="pgaptmp_001494"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGV7850130.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            61843..62021
                     /locus_tag="ID725_RS07475"
                     /old_locus_tag="pgaptmp_001495"
                     /pseudo
     CDS             61843..62021
                     /locus_tag="ID725_RS07475"
                     /old_locus_tag="pgaptmp_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(62048..62785)
                     /gene="espY1"
                     /locus_tag="ID725_RS07480"
                     /old_locus_tag="pgaptmp_001496"
     CDS             complement(62048..62785)
                     /gene="espY1"
                     /locus_tag="ID725_RS07480"
                     /old_locus_tag="pgaptmp_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HGV7850131.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATLDGATVDGATHLYDEVIIINKI
                     TPKKIDTEEVATKQSTAEQITDNAIIE"
BASE COUNT        15082 a        17245 c        15876 g        14659 t
ORIGIN      
        1 caaacggttt ctttttaagc aactcatctt caaccatgca taaagcgggt gcattcgctg
       61 ccgcatacca ttattcttga tctgacggaa gtctttttgt aactattcaa acttctttga
      121 tgtaaacaaa ttaatacaac aaacggaatt gcaaacttac acacgcatca ctgcgtagat
      181 caaaaaaaca atcaccgcac gaggtttcat gatggaaggt caacagcacg gcgagcagct
      241 aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg cgatagggac
      301 agggttattc ctgggtagcg cctccgtaat acagtccgca gggccaggga ttatcctggg
      361 ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg aaatggtggt
      421 cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg gcagctttgc
      481 tggcttcgct tctggctgga actactgggt actgtacgtt ttagttgcca tggcagagct
      541 gactgctgtg ggtaaataca ttcagttctg gtatccggaa atcccaacct gggtttctgc
      601 cgccgtgttc tttgtggtga ttaacgccat caacctgacc aacgtaacag tgtttggtga
      661 gatggagttc tggtttgcca ttatcaaagt tattgcggta gtagcgatga tcatcttcgg
      721 cggctggctg ctgttcagtg gtaacggcgg tccgcaggca agcgttagca acctgtggga
      781 tcagggcggt ttcctgccgc acggcttcac cgggctggtg atgatgatgg cgattatcat
      841 gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg ataacccgga
      901 gcaaagtatc ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt tctatattgg
      961 ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg ataccagtcc
     1021 gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc tgaacatcgt
     1081 ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct
     1141 gtttggtctg gcacaacagg gtaacgcgcc aaaagcgctg gcgtctgtcg ataaacgcgg
     1201 cgtaccggtt aacaccattc tggtgtctgc gctggttaca gcattgtgcg tattgattaa
     1261 ctatcttgct ccggaatccg catttggcct gttaatggca ctggtggtat ccgcactggt
     1321 gatcaactgg gcgatgatca gtctggcgca tatgaagttc cgtcgcgcca agcaggaaca
     1381 aggcgtggta actcgcttcc ctgctctgct ttatccgctg ggtaactgga tctgcctgct
     1441 gtttatggcg gtggtactgg tgattatgct gatgacccca ggaatggcga tttcggtata
     1501 cctgatcccg gtatggctgg tggtgttagg tatcggctat ctgtttaaag agaaaaccgc
     1561 aaaagccgta aaagcacatt aatctctcta cgccctcacc cgtacagggt gagggcaata
     1621 atctttacac caacgccccg taaatcgtca gtagcgcaat caccaccacg accacgaacg
     1681 aggttttctt cgccattgaa accgctgcct tcggcgtctc caccttatcg acatgcggtt
     1741 cacgcgccag agagaactgc gccagacgcg ttaatacctg atattgcgaa gtacggaaat
     1801 cacccagcga agcaaaccag gccggtaacg ctttctcacc atgaccgatc aaggcatata
     1861 ccacacctgc aagacgaacc ggcacccaat ccagtacatg aagcactgca tcaatgccgg
     1921 actgtaaacg atgatgcggc gtctggtatc gcgccagcca gtattgccat gcacgcaaaa
     1981 acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcagc cagaacagcg
     2041 gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca cgcaaatact
     2101 cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca gccatcgtgg
     2161 cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa tgaagacgaa
     2221 ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagcagc gtgggaacgt
     2281 tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc gcaataatgg
     2341 tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac gcttcaagac
     2401 gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt aacaccagta
     2461 aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc gcacgaaacc gtgcccaatc
     2521 aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt
     2581 attagcgata tccggatagc aatcaatcag tgcccgcgta accgccgcaa gctgttgata
     2641 ctgcgcatcg gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa tagaaaaatc
     2701 attgcagcgt tcgcgcccct gatactgaga gactcccgca tgccatgcac gtttatcgaa
     2761 aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg agacgcgcaa
     2821 atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag tgaataatgc
     2881 gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca ccaccagcag
     2941 ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc
     3001 aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg ctaatacccg
     3061 gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt taacatgccg
     3121 cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat taatctcgat
     3181 atccccggcg cggtggccca ggcgctgcgg gaagatttag gcggaacagt cgatgccaac
     3241 aatgacatta cggcaaaact tttaccggaa aattctcgct ctcatgccac ggtgatcacc
     3301 cgcgagaatg gcgtattttg cggcaaacgc tgggttgaag aggtgtttat tcaactggca
     3361 ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa cgccaatcaa
     3421 cccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg cactgcgctt
     3481 aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt cgaattactg
     3541 gaaggcacca acacgcagtt gttggatacg cgtaaaacct tacccggtct gcgttcagcg
     3601 ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct ttctgatgcc
     3661 ttcctgatca aagaaaacca tattattgcc tccggctcag tgcgccaggc ggtcgaaaaa
     3721 gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct ggaagaactt
     3781 gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga aacagaacag
     3841 atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc tggcaacgtc
     3901 actgacaaaa cactgcgcga atttgccgaa acgggcgtgg actttatctc cgtcggtgcg
     3961 ctaactaagc acgtacaggc actcgacctt tcaatgcgtt ttcgctagtt gctgacagat
     4021 ttatccgctc catagtgggc ggataatctc cccttcccca accttcttcg taacgcctcg
     4081 caaatttctt gtctttcgct gcaacgaaat aaaaaacctc ggaagcagct ttcccgtttc
     4141 gttttttgcc cctcgcctgt gctgatggcg tttgccaaag tagcaccaac caaatcaagg
     4201 agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg tggttattgg
     4261 catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc tgcgcaaagc
     4321 cgcactcacc gacatgctac aaacctttgt gccttaccgt accgccgtag agttgtgcgc
     4381 gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc cctcgcctac
     4441 caccacccgc tatgtttcag ccatgagtgt ggcaaagggc gtggtgtcgc tgaccgggca
     4501 agaaagtctc aatgggctaa gcgtcgtcat gacgccaggt tgggataacg caaacggcgt
     4561 caccggctgg gcgcgcaact gcaatattca aagtgacagc gcattgcagc aagcctgcga
     4621 agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc cacagctcac
     4681 ggccctgtgc ctgcgttatc agggagtctt gctggatgcc agcgaagaag tggttcatgt
     4741 tgcggtggtc gatgccccct cacatgagtt gctggacgca ttgcatttcg ctaccaccaa
     4801 acgtattgag atcacctgct ggacgcgcca acaaatggaa ggtcacgcca gtcgcacaca
     4861 acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt tgctgactcg
     4921 aacgttacaa tctgcgctgg aacaacgcgc gtctgatatt catatcgaac cagcggacaa
     4981 tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg atgtttcacc
     5041 ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg atattgcgga
     5101 acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg ccgtctcatt
     5161 tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt tgttacagca
     5221 ggtgagccag gcactggatg ttaacacgct gggaatgcag ccgttacaac tggcgggctt
     5281 tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta ccggcagcgg
     5341 caaaacggtc acgctttata gtgccctgca aaagctgaat accgctgaca ttaatatttg
     5401 tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc aaatccatcc
     5461 gcgtgccgga ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg atcctgacgt
     5521 catcatgatc ggagagatcc gcgatggcga aacggcagaa attgccatta aagccgcgca
     5581 aaccggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa cgctggtacg
     5641 tttacagcaa atgggggtcg cccgctggat gctatcatcg gcgcttacgc tggtaatagc
     5701 ccagcgtctg gtacgcaaac tttgcccaca ttgtcgccgg cagcaagggg agcccatcca
     5761 cattccagtc aatgtatggc cgtcgccgct gccccactgg caagcacccg gttgtgtaca
     5821 ttgctaccac ggtttttatg gtcgcactgc cttatttgaa gttctgccca taacaccggt
     5881 catacgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc acgcccgaca
     5941 ggcgggtatg cgaacgcttt ttgaaaacgg ctgcctggcc gtggagcaag gcttaaccac
     6001 ctttgaagag ttaatccgcg tattggggat gccgcatggc gagtaagcaa ctctggcgct
     6061 ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca gagagccgta
     6121 ctttactgct tatggcacta cagcaacaga tggttacccc actaagcctg aagcgaattg
     6181 ctatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat caactggcga
     6241 cgctacttaa agccgggtta acgctttctg aagggctggc actgctggcg gaacagcatc
     6301 ccagtaagca atggcaagcg ttgctgcaat ctctggcaca cgatctcgaa cagggcattg
     6361 ccttttccaa tgccttatta ccctggtcag aggtatttcc gccgctctat caggcgatga
     6421 tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg gcgcgtcagc
     6481 aaagagccca gcgtcagttg accgacaaag taaaatcagc gttacgttat cccatcatca
     6541 ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg ccggagtttg
     6601 ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg atcatgacgc
     6661 tggcagactt tagtggcgaa tggggctggc tgctggtgtt gttcggcatt atgctgacga
     6721 tagccaataa gttgctgatg cgccgcccga cctggcttat cgcgcggcag aaattgctgt
     6781 tacgcatccc gattatgggg tcactgatgc ggggacaaaa actcacgcag atctttacga
     6841 ttctggcgct gactcaaagt gcaggcatta cttttttaca gggcgtagag agcgtcagag
     6901 aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac gatatcagta
     6961 acggtcatcc catctggctg gcgttaaaaa atgccggtga gtttagcccg ctctgtttgc
     7021 aattagtgag aacgggagag gcctccggct cgctggatct catgttagac aacatcgccc
     7081 atcatcatcg ggataacaca atggcgctgg cggataacct agcagcctta ctggaaccgg
     7141 cgttgctgat cataacggga ggaattatcg gtacgctggt ggtggcgatg tatctgccaa
     7201 ttttccattt aggcgatgcg atgagtggga tgggataacg ctggcgtgtt gctccacacc
     7261 agcgttggaa gattacaggt tgttgaagat gcggttttct tgttcctgca cacggataaa
     7321 tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac aagctgaacg
     7381 caggccgccc aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg gcagcttaac
     7441 ggttttacct tctgctgcgc gatattcagc aacgccgcca acgtgacgtt tcatcgcaga
     7501 ctcggagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa cgatgcgacc
     7561 gccgctctct tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg caccgccacc
     7621 aaaggctttc gccacatcgc ccggcgtggt gcagccacca tcgctgatga tcattccgcc
     7681 cagaccgtgc gcagcatcgg cacattcgat taccgcagaa agttgcggat aaccgacgcc
     7741 tgttttgacg cgagttgtac aaacagaacc tgggccaatg ccaactttaa cgatatcggc
     7801 acctgagagg ataagctcct cacacatttc accagtcact acgttaccag cacaaatggt
     7861 tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt cggaataacc
     7921 attcgccacg tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa tctgtttagt
     7981 tttttcgaaa tccgcatcag acgtaccggt agaaaccatc acatgtttca gcacatcagc
     8041 ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat gcacagcagt
     8101 caaaatatca aaagaagcca gcgcagaggc catagaaaat gtgcctacgg tgtccatatt
     8161 tgcggcgata atcggcacgc cggaccagct ctgacctgaa tgtttgaagg tgaattgacg
     8221 ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga tgagaacgtc
     8281 tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg ggttaatggc
     8341 tataagggta agagcgcaac tccagtgacg ctatcatacg cactaatcaa tgcggcgcaa
     8401 gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt aaatagtgag
     8461 cgaacgtttc atcaatttcg ctttcgtgcg ctcattttta acgttttgat aatcatgatg
     8521 aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc attggcagtg
     8581 gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt gatgccgata
     8641 ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt gctgatcact
     8701 ttggcgctaa tatgattgct gctgatggaa cattgcagcg ccgggccttg cgcgagcgga
     8761 tcttcgccaa cccggaagag aaaaactggc ttaacgccct gctgcatccg ctgattcagc
     8821 aagagacgca acaccagatc cagcaagcta cttcccccta tgtactgtgg gttgtgccat
     8881 tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg gatgtcagcc
     8941 cagaaacgca acttaagcgc accatgcagc gcgatgatgt aactcgcgag catgtcgaac
     9001 aaatccttgc tgctcaggca acgcgcgaag cccgccttgc cgtggcagat gacgtcattg
     9061 ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac gcacactatt
     9121 tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc aggtcctttt
     9181 tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt tgattcagca
     9241 actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct tccgtaatgt
     9301 tagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt tgaaagaact
     9361 tgaccggcag caacgtaaac tccagacctg gattggcgtg cctggcgtgg accagagccg
     9421 tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt ccgcgccgcg
     9481 tatcgggcaa tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc gactgagcat
     9541 cccaggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc atctacccca
     9601 ggcgcagcgc gactgccagg tagaaacctg gattgccagc ctgaacccgc tcacccaggc
     9661 acttaccatg gtgctggatt taattcgcca gtcggccccc ttccgtaaac aaaccagcct
     9721 gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga atctgtcgct
     9781 cgattcacag ctttatccgc aaatttccgg acataagagc cgttttgcca ttcgttttat
     9841 gccgctggac actgaaaacg gacaggtacc ggaacgtctg gatttcgaac tggcctgttg
     9901 ctaaggagta aaaatgtcag aaactattac ggtgaattgc ccaacctgcg ggaaaacggt
     9961 ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc agctgatcga
    10021 cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc tttccgaaag
    10081 cgatgactgg agcgaagagc caaagcagtg acatttgtct gatgccgcac gtaggcctga
    10141 taagacgcgg acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc
    10201 cttatccgac atacacacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat
    10261 catcggcatt aagaccgact agcgacatcc acttaccggg ttgcccttct ttaccccacg
    10321 gctccccttc ccagctttcg accagccaga accacagagt tatatgcctg tccgggaatt
    10381 catattccag tttttcaaat agcgaaaaat gttgtggggt aatcccgact tcttcctgaa
    10441 gttcacgcac caccgcctgt tccggcgttt cacccatttc aattttaccg ccgggaaact
    10501 ccagtttatt cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt tcattgttct
    10561 cgttgcgaat aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa
    10621 ggcgcagaat cctgcgcctt ttacttcaac agttagcttt tattgcaggc ggccatggca
    10681 ctgcttgtat tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgctc
    10741 accggtttgc gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg
    10801 ctgcatttgc gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc
    10861 ttcaggcata cgtacctgaa ctttgctcag cgtactgata acttcatatt tcaacgactc
    10921 cagcatcgct gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg
    10981 tgcatagcca cgcaggtgga taccctgacg cagatagtcc atcgctgcca ggtgctcttt
    11041 ccacagggag tcaagcgttt gcagcatgac gcctttctcg aagtgacgca tcatctcagc
    11101 accaaccact tcttctttac gctgatacac ttcgatggac tgcgccagaa tgcgctcacg
    11161 cagcgtctct tcatgcagtt ctggttcttt atccagccac tcagcaattg gtaaatcgag
    11221 gtcgaaatcg ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagcga
    11281 ctgtggcgga atgtaggcat caatggtcgc tttgaacaca tcttcacgaa tgctgttaat
    11341 ggtttcgctc acatcgctga catccagcaa ttcgttacgc tgggagtaaa tggcgcgacg
    11401 ctgatcgtta gccacgtcat catattccag cagttgctta cgaatgtcga agttacgact
    11461 ttcaacttta cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc
    11521 gcctggcttc atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg
    11581 catcagcgca tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc
    11641 agaacgaccg cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat
    11701 atgcaggcca cctgctgcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt
    11761 ttcaatttgc tctgcggtcg gattttccag cgcggcaact tctgcctgcc agctaccacc
    11821 gagcacaata tctgtaccac gacccgccat gttggtcgcg atagtcaccg cagccggata
    11881 acctgcctga gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacattgtg
    11941 cttaataccg gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt
    12001 acccaccagc accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg
    12061 aattttttcc gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg
    12121 gttggtcgga acaacgacgg tatccagttt gtagatggag ctaaattcga aagcttcggt
    12181 atcagcagta ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt
    12241 gatcgaagcc agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc
    12301 ctggtgcaga ccatcggacc agcgacggcc ctgcatggta cgaccggtgt gttcgtcaac
    12361 gatgataact tcaccatctt taacgatgta gtcgacgtca cgggtaaaca gcgcatgagc
    12421 gcgcagcgcc gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc
    12481 cccttcatcc atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc
    12541 ggtcaggttc acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt
    12601 ttcggagtct tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata
    12661 catttccgag ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat
    12721 caggatggag tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc
    12781 agggctgaac gccatattgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta
    12841 agtgatgtca gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat
    12901 accgacagtc aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc
    12961 caggtagtcg ttgacggtaa ctacatgcac gcctttaccg gttagtgcgt tcagataagc
    13021 aggcagcgtt gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc
    13081 gttaagaacc ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt
    13141 acttgcctca cgtaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt
    13201 ttccagacgt gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg agagtttttc
    13261 catctccggt tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg
    13321 atcgttacga ctaccgaaaa ctttagttaa taatttgatt agcataataa aatctcaaac
    13381 gccccgcgtt gcggagttaa taaaatgaag taaaggttta ttgttgttag ctgaggcgtt
    13441 gagggccagc acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt
    13501 gtggggtaaa atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg
    13561 tcagcagcgc gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt
    13621 cttcagcaac gggcagcgtt tgcggtgcca ttgcgaagga aagatgacgg attaccgtgc
    13681 gaatggcatg ttgatgccag taatctacgg aatagttcga attcgggcgg cgcgtgttcg
    13741 cttccagcaa ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg
    13801 tcgcttttgc gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg
    13861 ccgcaaccat ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc
    13921 gcgtcagtat tccactcacg ttattgccat cccgatcgta ttcaaacgta tttagcgcac
    13981 aaatcttatc attaaagcct gaagtccaca gcacgaatga agataagttg tccgtaaaag
    14041 tgcttaagaa aacagcgttc gcaccgtttt ttatagctct tataaaattc aaaaaaaaac
    14101 gactggttca cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta
    14161 taaatgtcgt tatgccagta cggctgaagg cgctttgaag gccaacggca gttctgcgtc
    14221 gtcctggaag gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt
    14281 gttattcagt gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca
    14341 catgaacaag tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg
    14401 caggccgtct tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc
    14461 gcccaggcac aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg
    14521 cgcacggctg atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt
    14581 gctggaatca atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta
    14641 cggcttaaat tcagcccact tatcgccatc ttcgacacga acagtctctt tgatgcgaac
    14701 aaattttttg gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg
    14761 agcggcgctg ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc
    14821 gatgcccaag cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg
    14881 ctcgttgacc agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa
    14941 atctaccggt ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg
    15001 gcgtaacgtc agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat
    15061 acgtttaagt gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga
    15121 agtgtactat acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag
    15181 tcaattctta atcagcttgc ttacgcagga atgctgggat atccagataa tccggctctt
    15241 tcgcagtttg cggcgcattg tcattcacga ctttagcaac cggcttctgc tcctgggtca
    15301 gcggagccat cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg
    15361 tcaccagagt gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc
    15421 gcagctcgtc attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg
    15481 aagcaaatgc acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc
    15541 ccgctgtgat gttaaccagc acgccgcgtg cgccagacag gtcgatatct tccagcagcg
    15601 gagaagagat agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag
    15661 aacccatcat tgcgtagccc atttcagaca ttacggtgcg tacgtctgca aagtccacgt
    15721 tcatcaaacc cggacgagta atcagttcgg cgatgccttg cacagcgcct ttgagtacat
    15781 cgttcgctgc gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt
    15841 tgtcgttcgg gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct
    15901 ccgcgaatgc catacgcttc ttgccttcaa agttgaaggg cttagtgacg acagcaacgg
    15961 tcaggatacc caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac
    16021 caccacccat acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcagtg
    16081 catcgcgatc ctcatctgcc gcattgcggc caacttctgg attagcgcca gcgcccagtc
    16141 ctttggtgat accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt
    16201 gtgcatcggt atttaccgcg aagaattcaa caccttcaat gcgctcgcgc accatgtgtt
    16261 caacagcatt accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa
    16321 gttccattgg ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc
    16381 atcgtcggcc tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc
    16441 acgagccaac tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact
    16501 ctttcccata gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg
    16561 ttaaaccggt aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag
    16621 cacaggctgc aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca
    16681 ggtgatgttt aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga
    16741 ccaggttgag cagctcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca
    16801 gactccgcgg cggacgaccg cctacgctcg gcacttccac gctctcatct tttccaacga
    16861 tggaacccag cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc
    16921 caaaggcgta agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt
    16981 ggcgcaatgc cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga
    17041 cgcagacacc cagttcacgt tcatcttccg tcaataccga ataacttgat gccagaccgg
    17101 caaatatcag ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg
    17161 ccatatcgtt gtggcatgtg atcaggtgca cttttgcctg catccgcacg cccgaaagcc
    17221 ctaccggatt cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca
    17281 gcacacgatg ctcatcgcgt acacgcaccg atttcgcggt atggacgacg ttttccacat
    17341 cttcttgcgt tacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga
    17401 tgtgcttacc agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg
    17461 cctggtcaat ggcacgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt
    17521 tatccatacc acgcgacggg cagctgccca cgccaatgat attgaccata ccgtcgggca
    17581 gaacttcccc tactaaagcg gcaaccttcg cggtaccaat ctccagtcct actaccagtt
    17641 ttctgtccgt cgccttgatc attgttgttc tgcctgtgcc tgattttgtt gctgagtaga
    17701 ttcctctggc ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac
    17761 gtagctaatc cgtttgccat cggtttgcgc ctgctgctgt agaaccggat aaagttctac
    17821 aaagcgagcc aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt
    17881 cagcgtcaac tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc
    17941 ctttgccagc atctgcccca tttcgcgata gccctgcaac acttcattgg cgctgccttc
    18001 cgggccatac agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt
    18061 atttccttcc gcgtctacca tgtgttgatc attccaccgc gcaatcggca catattcaac
    18121 cagatgaatc ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg
    18181 cagacgttgt tctatttgcg tctggatgat gttgacatcc tgggtcataa aggtacccgg
    18241 ctcacccaat gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc
    18301 ggtcaacacc agctttgaga gcggcaggcg ttgcgcatct tccatccagc ccaacacgac
    18361 ccagccgctc accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg
    18421 cgttccatta ttgcggcgag aagaaacctc ttcttcgctg tttcgcgtgt tcagagcagc
    18481 ctgcgacata ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac
    18541 ctgcctgacg tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg
    18601 cttccagcag ataaaactgt ccatcgctgt ccagcataac gtcaatacgt ccccatcctt
    18661 tgcaacctaa cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg
    18721 cttccagacc tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat
    18781 catagaaggt tccggacggt tgaatacgta ctgacggtaa aatttcttca ccgagtatcg
    18841 caaccgtgaa ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa
    18901 atgccaatct taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac
    18961 tggaaccttc gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta
    19021 actgcttatc actcaggcct ttttcaaact ctacgcgggt taacgctacc cacggcgcga
    19081 ccggtaaacc ggcaccttgc cacagaagtt tgctgcgtag tttatccatt gaaagcgcag
    19141 atgccatcac tccgctgccg gtataaggca agcccatcag ctcgagcatc ccctgcagcg
    19201 taccatcttc accgccgcgg ccgtgtagcg cgataaacac tttctgaaag cccatcgact
    19261 tcagttgcgt cacgtcaact tctttcgggt cgacaggata tgcgtcaata ccgccttcac
    19321 gcagcccggc taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaagtcc
    19381 cgcccaacag gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc
    19441 agtttgattt cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga
    19501 atcaggtcgt taccggttaa taccggtgcc agcatctcgg ctacctgcgc agggtccggc
    19561 accagaatgg gatctatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc
    19621 gcacccggaa ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc
    19681 tgcgtcagca cattggcgaa atcatcatac aggtcgcgtg tacgggtaaa acggtgcggc
    19741 tgaaacagca ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt aatggtggcg
    19801 tccacttccg tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca
    19861 ttcactggct ccagcgggaa ttcaccgagg aaatcaaaac ggcgaccagt cccctggaag
    19921 ctctccagcg cacgcaaaat agcctcgtcg tcaatgccct cttccgtagc aaccgcaacc
    19981 gcagctgcgg cgttcagcgc gttatgacgg cctggcgcat tcagggtgac gcgcatcggc
    20041 tctttgtcct ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct
    20101 acacgcacgt cggcatcttc gctgaagccg taagttgtgg tctgacgtcc cactcgcggt
    20161 aacaattcgc ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaacggcagg
    20221 ttgtgcagaa aattaataaa agtctgtttt aaattctcaa agtcgccctg gtaggtatcc
    20281 atgtggtcgg cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat
    20341 gcatcactct catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc
    20401 cccgccgctt ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag
    20461 atgctggaga ccatcgcggt agttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca
    20521 tgacgaaaac gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct
    20581 tcatgagctg cgacaatttc cgggttatcg gcagaaatgg cgctggaaac aacgaccacg
    20641 ctggcatcac gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc cagattcatt
    20701 aactgctgcg tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg
    20761 gccagaactt cggcaatacc gcccataccg gcaccaccga tgccgacaaa atgtatgtgc
    20821 cgaacgcgac gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt
    20881 ctttacgcca ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc
    20941 gggcagcccg actcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc
    21001 gggcgcgttc tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga
    21061 cagcatccac gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg
    21121 gtagcgcatt ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta
    21181 gtcctgccgc ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg acgacatccg
    21241 cccacgcata cgccgccgcc atatcatcaa taaattcagt cactttatgc tgcggttgcc
    21301 ccgcttcggc atacgcctgt tcaacggatt gttgcgaacc tttgccgctc tgatgccaga
    21361 tagtgactga atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg
    21421 cgccctggga accacccact accagcacac gaaccggacc ttcacgtcca gccaaacgtt
    21481 gctgcggcaa cggtagcgcc aacacatcgg tacgcaccgg gttacccacc acttccgcat
    21541 tggggaaagc acctggaaac gcctgcatca ctttggtggc aatcttcgcc agccatttat
    21601 tggttaagcc cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg
    21661 cggccagacc accaggacct gatacgtagc cgcccatacc gagcaccacg tcaggtttgt
    21721 acgctttcat aatcgcccgc gcctgacgcc aggcattgaa gatacgcagc ggcgcagcta
    21781 tcagtgcttt tatacctttt ccacgcagac cagagatacg aatgaaatca atttcgatgc
    21841 catgttttgg cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc
    21901 aaccctgagc cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc
    21961 cgcctgccat caccattaat cgctttcctt gagcactcat cgtgaacctc gtacaaacgc
    22021 ctgcgctttc tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga
    22081 cataatcagt aagctcgaac caccgtaact gatcagcggc aatgtcagac ctttggtcgg
    22141 taacatcccc gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat
    22201 agaacaggcg agaaaaccgg aaaaacggtg gtcaatttct aatgctttac ggccaatcga
    22261 catcgcgcga aaagcgacga agaataccat taaaagtgcc agcaccacac cgacataccc
    22321 cagttcttcg ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag
    22381 tttttgtaca gagttaccta aaccttgtcc ccacagttcg ccgcgaccaa acgccatcag
    22441 cgattgcgtt aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt
    22501 aacacggcgg atacggtacg gttcggcgag tatcagcaac acaaccgctg aaatgcccat
    22561 accgataatg gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt
    22621 agtcacaaac aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa
    22681 caccagaatc acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcacc
    22741 tttacgcacc agatagttgg cgatatagca aaacagcgac agttttgtca gctcagcagg
    22801 ctggatacgc agcaaaccga gatcgatcca acgcgatgcc cctttaaccg agctccccac
    22861 taccagcacg atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg
    22921 ccagaactcc atcggcagac gcagcgtaat gatcgccaga ataaacgcca aaatcagata
    22981 gacaccatca cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc ctatgggcat
    23041 tgacgccgag gtcaccatga taaagccaat cgccgcgagg ccgaaggtca gccacagtaa
    23101 ggtgcgatcg tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc
    23161 ctttagcgcc gtggagatcc agaccaggat actgaatcct ggcaggcgcg gcattttcag
    23221 gcgagggaga gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct
    23281 cgttgttcaa agttcttgaa ctgatcaagg ctggcacagg ccggggagag tagaaccata
    23341 tcgcccggct gaacacgcgg agccagcaag cgcatcgcct gttccatagt ttcggtttgt
    23401 tctgccactt ccgggcgtag cgccgccagc tgcacgccgt cacgaccgaa acaatacaga
    23461 cgtacgttat cgccattcag gtaacgcgcc agtgggctaa agtccgccga tttaccatcg
    23521 ccgcccagca acaaatgcag cgtgccgtct acgtgcaggc cattcagcgc cgcttccgta
    23581 ctgccgacgt tggtcgcttt cgaatcgtta atccaacgta cgccgttatg ctccagcaca
    23641 acttcaaagc gatgcggcag accagtgaat gtggttaacg ctttcaggct gctggcacgc
    23701 ggtaaccctg cggcatctgc cagcgccagc gccgccagcg cattggtgta gttatgctgc
    23761 ccggaaagtt tcatttcttt cacgttcagc actttctcgc ctttcacccg cagccaggtt
    23821 tcgccctgct gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt
    23881 tcatccgcac cgcgaatcgg cattgttaag gcatcatcag cattgacgac acaaactttc
    23941 gcattttcgt aaatacgcaa ttttgccgca cggtattgct gtaaaccaaa cggatagcga
    24001 tccatatgat cttcagtcac gttcagaatg gtcgctgcta ccgcctgtaa gctggaggtg
    24061 gtttccagct ggaagctcga cagttccagt acgtacagtt cgcactcatc atccagcagc
    24121 atcaacgcgg gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttcgcc
    24181 atttcaccca ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc
    24241 ggtgcttgtg cttcgcgaca gaacagctcg atatcgccaa cgatttcgat tccggcatca
    24301 gcggcagcgc ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct
    24361 gccgccatca gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat
    24421 ttatccaggc caggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg
    24481 aggaaaaagt ccacgcagga aagcccggtg aggcccaggc cgataatgac aacattttta
    24541 ccctggtaat cagccatgat taacgtacct tcagcgttgc cagaccaatc aggaccagca
    24601 tcagcgaaat aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat
    24661 agtggtgatg aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga
    24721 cctgcaggat gacggaaagc gtttctacca cgaacacgcc ccccataatc accagcagga
    24781 attcctgacg cagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccta
    24841 catcgcccat aaagacctgc gccggatagg tgttaaacca caggaagccc agccctgccc
    24901 cgactatcgc ggtacagaca ataaccagtt ccccggcgtg tcgcagatat ggtatgtgta
    24961 aatagctggc aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga
    25021 caaatacggt cggcataatt gccagaccgt caagaccatc ggtcaggttt accgcgttgc
    25081 cagtacccac aatgacgaag taagccagca gaatgtagaa cagccccagt tgcggcatca
    25141 catctttaaa gaatgggacc accagctgcg ttgcgggcgt gtctttgcca acaaggtaca
    25201 gcgcgaaggc gacacccagc gcaatgaccg acatccagaa atacttccaa cgagcgatca
    25261 accctttggt gtctttacgc actactttgc gataatcatc aacaaagcca ataacaccgt
    25321 aacctaccag caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca
    25381 gtacggagat cacaatcgcc gtcaggatca taatcccgcc catggtcggc gtaccgcgct
    25441 tgctgaagtg tgattcagga ccgtcgttac gcaccacctg accaaaggaa agtttttgca
    25501 aatgagcaat catacgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga
    25561 cgatggcgcg aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat
    25621 gttcggccag ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat
    25681 ggcggcacta cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga
    25741 tttaagacgc gtaattaacg cagttttctc agcaaaatgt tcgccaacgc cgctggcggt
    25801 gctgatagca tggctttgtt tacccacgct taacacgcgg tcaataccag cagcttttgc
    25861 cgcctcgccc acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc
    25921 caccaccagc acgcggtagc ccggcatttc agccagtacc tgaacagcgg cggtcattga
    25981 accgacattg gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttgaatggg
    26041 gaacaaacgg cctggaacag ctttcagatt tgccagcccc gctttgatag catcaagcgt
    26101 tgcgcccacg gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc
    26161 cggcaacggc agcagaacat ccacgctacc ggttggggtt tgcagggtaa attccgtacc
    26221 gtgcgaagtc acatggatat tggtggcggt gaaatcgctg ttggcggcat taggtgagaa
    26281 acgccacact ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc
    26341 gttcataatg gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc
    26401 gacacccgca agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc
    26461 agcttccggg cgagtcagac tcacagtcca gacaatttcg ccctgatggt tcgcgccaag
    26521 ttcaataact gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat
    26581 gtcgttgttg agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc
    26641 cgtcatctct ttaacggagg ttttgccgga ggaacctgtc agagcaacca cgcgcgccgg
    26701 aacttgctgg cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat
    26761 taactgcggc aggtcgatat ccagcggacg gctaaccagt agtgcgcctg cgccgccagc
    26821 tttcgcctgg tcggcaaaat catgagcatc gaaacgttcg cctttcaggg caacaaacag
    26881 gcagcccggc gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc
    26941 ttgcagttca ccgttgagaa tgtcggtaag ttggctaagg gttacgctaa tcatgcaatc
    27001 acccccagca gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca
    27061 atctggtaat cttcatggcc tttgcccgcg accagcacca catcgttctc tttagcctgc
    27121 ataacggcgc aagtcaccgc ttcagcacgg tcttccatca ctttggcatg tccggcatct
    27181 aacattcccg ccagaatatc gttgatgatg gcacgcggct cttcggtacg cggattatcg
    27241 tccgtcacca ccgccacatc agcaaactct tcggcaattg cgcccatcag tggacgctta
    27301 cctttatcgc gatcgccacc acagccaaag acacaccaca gcttgcccgc acagtgcaga
    27361 cgcgccgcct gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc
    27421 gtcggtttgc ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgagca
    27481 gcggttttca gcagatcagc cagtggatag cccagcgcca gcagcgtcgc cagcgcgagc
    27541 agcaggttgc tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccaa
    27601 cttgagctaa agcgaatagt cgcaccgctg tcgtgatagt tcacttcggt cgctttcaac
    27661 cagcgtccgt gacagttcgg attgatatga tcttccattg ataccgcaac tgcatccggc
    27721 agttttgcca gccagcggcg gcctacttca tcgtcggcat taataatcgc ctgaccgcaa
    27781 tgatgttcag aataaagcag ccatttcgca gcttcgtagt gttccatatc accatgataa
    27841 tcaaggtgat cgcggcttaa gttagtaaag accgacgccg caaatttcaa tgccgccaca
    27901 cggtgctgta ccagcccgtg ggaggaaact tccattgcgc aaaaagtcgc gtcctgatcc
    27961 accagccccg ccagctcatg ctgaacatca actgccgaac cggtggtatt ttctgtcggg
    28021 atcactttcc ccagcagacc gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc
    28081 agttggctcc actgcgccag cagctgggtc gtcgtggttt tgccgttggt gcccgttacg
    28141 cccacgagac gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa
    28201 cgctcgttga gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg
    28261 tcggtcgcct cacctttcgc ctctgcaata atggcagcca caccttgcgc tatcgcctgc
    28321 gggatatatc gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc
    28381 gcagccacac ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc
    28441 cacggagcaa gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat
    28501 cacaaattca tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggttcgcaa
    28561 tacgccgccc atgatggcac caaagaccgg cgcggaaacg gcgccgccgt agtatttacc
    28621 cgcctgcgga tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac
    28681 acctgcggtg taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc
    28741 ggtaccggtt ttaatggcaa tacgatagcc tttaatcgcc gccttcacgc cgccgccacc
    28801 cggtagcgcc acgctttcca tcatatgcac cacagtgcgg acaatagatt ccgggaagac
    28861 acgttcaccg ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta
    28921 gctgccgata gttgcgtaga ctcgcgctaa ctgtaacgga gttaccatta gcccgtagcc
    28981 gaaagagaag gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact
    29041 gcgttctccg accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc
    29101 tactaacgct gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt
    29161 ctgtaatact ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc
    29221 gttaattcga taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa
    29281 cgcggtcatc accaccatcg gtttaaccgt tgaacccggt tcaaacacgt cggtgatggt
    29341 acggttacgc atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct
    29401 gttagccatc gccagcactt caccggtgtt gacatccacc agcacggcgc taccggactc
    29461 agccttgtta aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc
    29521 atcaatactc agcgccaggt tgtgcgcagc ctggctgtca gtagaagaaa tatcttcaat
    29581 tacgcgacca tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt
    29641 atcgaaactc ttctcaacgc cctcaatacc ttgactatcg acgttagtaa agccgatgag
    29701 gtgagcagtc acttcgccgg atggataata acggcgagat tcttcacgca gatgaattcc
    29761 cggcagtttc agttttttga tgtagtccgc catgtcaggg tttacctgac gcgccagata
    29821 aataaagcgc cctttcgggt tggcgttaat gcgggcagaa agctgatcca gcggaatatt
    29881 gagcgcgtta gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac
    29941 ttctttcggg tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga
    30001 acggtcagta atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc
    30061 gccctctttc accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag
    30121 aaaagccagc gccaggagaa tacagccgca taacaacgca aaacgccaac tgataaagtt
    30181 ggcatgttct tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt
    30241 ttatccttat ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag
    30301 cttttccgtg gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat
    30361 caggttgcgc cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc
    30421 ggtcagtaaa cgggtatggt gcgccgtggt taccacggtc accgccgtca aaataatgca
    30481 aatgaacagg cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg
    30541 caatgcatgg cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac
    30601 tctgctgatc atgcgttcgt cctctctgca atacgcagaa ctgaactacg ggcacgagga
    30661 ttctcagcca cctcttcttc gcccggcatt aatttgccta gtgctcgcag ctgacggcca
    30721 cccagttttt tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg
    30781 ctgttttcac gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc
    30841 gaaagccgcc cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc
    30901 tccagttcac tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc gggatgttta
    30961 aacttatctt tcaccggcgt tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc
    31021 atcggctgtt cgcggttacg ctcgacaatg gctcgggcaa tgcgtttggc aaaacgctct
    31081 tcaccatagg ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca
    31141 gcggctgact gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag
    31201 gaaaagccgc gttcagcatc atcaagttgc ggtgaagaga caccaagatc gaggagaatg
    31261 ccgtcgatct tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc ggaaaaaggt
    31321 ccgtggatga tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg
    31381 tcgcgatcga tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag
    31441 tgaccaccgc gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca
    31501 ttaacggctt catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga
    31561 caagtcctgc agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc
    31621 cttgacctgt tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac
    31681 cagcatcact tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg
    31741 acctgcgcca tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg
    31801 ctcaaccggg ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg
    31861 cagggggtaa agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca tttgaccggc
    31921 agcgttctca agcagctgtt cccgataacg ggtaggcact gataagcgcc ctttgctgtc
    31981 gagattgact aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc
    32041 ccacaatttc ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac
    32101 aaggcatacc gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac
    32161 gtccaacgtg cgaagcaaat taacgttatg aatagctgta agaaaaaaac cctcatcgtc
    32221 atcaccgttt taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac
    32281 gcgagcggca ttttaggaca tatcttcccc ggttaacagt ccctgttgcg tctgtgtagc
    32341 gcttgatgcc agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat cggcaattca
    32401 tctctgcatg taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa
    32461 tttcagctgg tgagttaatt aactggcggg tttaaaagca tttatcaata atagtcagga
    32521 atagtcttat ttactttaag gatattgatg tccagtcccg tactctacgc gccagagtga
    32581 aattcacctg gcgcgtattt ttgttcgcag cttagctacg gctgagcaca ccgcggcgat
    32641 agagattacg tttaatgcgc gttaaaccag gttttggctt acgcggttcg tccaggcttg
    32701 ccaggacaat ctccagcaca cgctctgcga catcgcggtg acgttgagcc actgccagca
    32761 ccgggcactg taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt gccaggtcag
    32821 aaggcagttt accatcgcga cgtagcgtga catccatcac cccttgcaac aacgcaaacg
    32881 acgttgtgaa cagcgcctgc ggcatcggat gcgtttccag ccatttttcg aataactggg
    32941 cagccgcctc ccgctcatag ctgttggcat acaggaaatg cacttcgcgc ggatcatctt
    33001 tccaggcagt acggaaacct tgttcacgca ggaagctgac agaaagctcc ggcagcgcgc
    33061 caagataaag caccgtctcg gcgggaaact tacgtaactc ttccgccagc atttcggcat
    33121 catcctgatc ggcaccaacc acgctggtaa agtgttcacg atcgagggcg cggtccagcg
    33181 cgacaatcgg gaacgggtcg ttagcccagc gttgataaaa aggatgctca ggaggcaacg
    33241 acgtcgaaac aataatggca tcgacctgac gctgtaaaag gtgctcaatg cagcgcattt
    33301 cgttgtctgg ctgatcttct gagcaggcaa tcagcagttg ataaccccgt tgccgcgcct
    33361 ggcgttcaag atagttagcg atgcgggtat agctggtgtt ctccagatcg gggatcacaa
    33421 gaccaataga acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc gggtggtaat
    33481 tgtgctcacg caccacagcc atgacttttt caacggtttt gtcactcaca cggtattgct
    33541 tcgctttgcc gttaataaca tagcttgcag tggtccgcga cactcccgcc agccgagcga
    33601 tttcatccag tttcacaatt gccccttgcg taaaatgtaa aaaccatagc cattgtactg
    33661 gtatgggtta aattcattaa catctaagcg cagaaaatta actgcggcaa ctgcttttat
    33721 tccaggttcc cactgatttc gcaaaaaaag cccaacgtga catgttgggc tgtaaattgc
    33781 gcattgagat cattctcaac gcattatttt atcgccgcgc gaaagtccga ccacaccaga
    33841 gcgagcaact tccacaattt tcgccacatc gcgaatcgat gctaaaaatg catcaagctt
    33901 atcgctggtg cctgctaatt gaacggtata aagcgagggt gtgacatcaa taatttgccc
    33961 acggaatatt tccgtattac gtttaacttc gtcacgcccg taaccgctgg cctgaatttt
    34021 caccagcatg atttccctct caacatgcgc gccctgcccc aactcactca cgcgcaagac
    34081 atcgaccagc ttgtataatt gcttttcgat ctgctcaagc actttttcat cgcccacggt
    34141 ctggatggtc atacgcgata atgtcggatc gtcggttggc gcaacggtca ggctttcaat
    34201 gttgtagcca cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg attcattttc
    34261 gagtaagact gataatatcc ggcgcataat caggttctct ccgttttgct taaccacatt
    34321 tcatccattc cgcccccgcg aatctgcatc gggtagacgt gctcactgcc atcgacggtg
    34381 acatcaacaa ataccaggcg attattacgc acctgttcaa gcgcctcgct aagtttgctt
    34441 tccagctctt gcggatgaga gatctggatc ccgacgtgac cataggcttc cgccagacgg
    34501 acgaagtctg gtaaagactg catataagat tgtgaatgac gaccggaata gatcatgtcc
    34561 tgccactgct tcaccatccc caaatagcgg ttattaagat taaccaccag caccggcagt
    34621 tcatattgca acgctgtaga cagttcctga atgttcatct gaatactgcc gtcgccagtg
    34681 acgcaaacca cggtttcttc tggcagcgcc attttgacgc ccagtgccgc aggtaaacca
    34741 aagcccatag tgccgaggcc accggagttg atccaacgac ggggtttgtc gaatggataa
    34801 taaagcgcag caaacatctg gtgctgcccg acatcggacg taacgtaagc gccccccttc
    34861 gtcaaccgcc aaagagtctc gatcaccgcc tgcggtttaa tcttttcact gtgagtgtca
    34921 tatttcaggc actgacgagc gcgccactgt tcaatctgct gccaccagtc gcggatctca
    34981 tccagcggtt gatgggcgga ttcttgcgac aagagttcaa gcatttgttc gaggacctgg
    35041 cgagcatccc ccacaatcgg aatatccgca gtcacggttt tagaaatgga agtaggatca
    35101 atatcgatat gcagaacagt ggcatttggg cagtactttg ccagattgtt agtggtgcga
    35161 tcgtcaaagc ggacaccgac agcaaaaatc acatccgcgt tatgcatcgt catattggct
    35221 tcgtaggtac cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc cggaaacgct
    35281 cccagcccca tcaatgagga aacaacgggc agatttaacg cctccaccgt ttctttcagc
    35341 tgttgatggc agcccgccgt gattgcccca ccgcctacgt agacaaccgg tttttttgcc
    35401 gccaccagcg tttgcagagc gcgcttaatt tgccctttat gtccggtagt agtgggattg
    35461 taagaacgca tactgaccga ctccggccag acatagggta atttgttcgc cggattaaga
    35521 atatctttcg gtaaatcaac gaccactggt cctgggcgac cacttgccgc cagccagaaa
    35581 gcctttttca gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc
    35641 accggtcgcg aaatccccac catgtcgcac tcctgaaagg catcgtaacc tatcaacgag
    35701 gtcgctacct gcccggaaag gacaactaat ggaatggaat ccatataagc ggtggcgatg
    35761 ccggtaatcg cattggtcgc ccctggaccc gacgttacca gcacgacgcc gacttccccg
    35821 gtcgcgcgcg ccagaccatc ggccatatgc actgccgcct gctcatgacg aactaaaaca
    35881 tgaccgatac cacccacggt atgcaatgca tcgtaaatat caaggactgc gcctccggga
    35941 taaccgaata cttgtttaac gccctgatcg ataagcgatc ggacgaccat ctcggctcca
    36001 gacaacatct ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa acacattaac
    36061 cgtccgatct tgcttaagca attggcagcc agagacataa acgccgggta ataagaataa
    36121 acaggaggaa aggtgaaaag aaacgataaa atcctccatt acgaaggata aaaatgataa
    36181 taaatcggct aaatcccacc acttacagca atttctctat ttttttaatt aatcctaaaa
    36241 tagagagccc acacattcag aataaaattc tacactcaat aaaaaatgca gaataaacca
    36301 gacattcatg tctgacctat tctgcattca gttagcgttt gcaaattgag actaattgct
    36361 cttccatcca ctgatggcct ttatcacgcc cggcagcttc atgccaggag agataacagg
    36421 ttctgctatt tagttttaac ggcagcggta atacctgtaa ttctaaggat tcagcgaact
    36481 cttcagccag ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc acgctaagta
    36541 cgctcatcat tgccatgccc tgatacgcga tactggcttg cttatctacc gtgtcatacc
    36601 aaggttgact aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt tcgttataaa
    36661 catcatgttt cagtaacggg cccttaattg ttgggtgatt tttgctggct accagcacca
    36721 tttcatcttt aaataatggc acgctggtaa attcaggacg atggaagtct tcataactaa
    36781 tcacaaactc cgtttcctga taacgcagct gatgttcagt gttctgattt aatgaagact
    36841 tgaacataac atgtatattt ggcgcaatct gctcaatgtg attataaatc tgcgaggtca
    36901 gaatactgtc taacgggctg caaacacaaa gatgaaatac acgttcactg ctcgcgggtt
    36961 caaaacctga accaggcaat tcattttgta ctagttgcaa tgcctgacga actgaaccaa
    37021 aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc ataacgaaca aaaagctcgt
    37081 cattaaacat caccttcagg cgtgcaacag cgttactgac cgcaggttgc gacattccca
    37141 gaacatgagc agcacgggta atgttttgct cctgcatcac ggcatcgaaa acggttaata
    37201 agttgagatc gaccatgcgt agctgtggct tgctgaactc cgccgtctct ggatgatctg
    37261 tttgtacctc tggcatactt aactccactg tcacacttaa ctccctttcc cttattggaa
    37321 tgcagagaaa taatcctcga gaatatgatt tatcatgcat ataaaataag aaaaaggaaa
    37381 atgagtaaaa ttaggaaaac ataaagatac gggttcttat aaaaacagaa tccattgccg
    37441 tggaaagcgt catttaccac agcataataa tccataatgt tcttgttgcg taaacaatct
    37501 aatcataact accgcgaata ctcaatcatc cacaaaatgg attaaatgtg ctttttaata
    37561 atttatttat atatttaatg cattaattct taacattaat tgatcaataa tattcaccaa
    37621 atcaatatca aaaaatccgc aaaacatata attcaataca aatcatcagg ataggttttg
    37681 caacgcgtgc attttgtccc ctttttcctc gtggattaga tgcaaaaatt tattctgaaa
    37741 tatgtcaacc gatgaaaagc atcggtagtt aagcagaaat taatatcgct tgttttaacc
    37801 acagcagcac aattagctaa tcttacggat gcagaactca tgctggcggg acgtttttat
    37861 tgcgtcaggg ttgacatccg tttttgtatc cagtaactct aaaagcatat cgcattcatc
    37921 tggagctgat ttaatgactc acatcgttcg ctttatcggt ctactactac taaacgcatc
    37981 ttctttgcgc ggtagacgag tgagcggcat ccagcattaa gccagcacgc agtcaaacaa
    38041 aaaacccgcg ccattgcgcg ggttttttta tgcccgaagc gaggcgctct aaaagagaca
    38101 aggacccaaa ccatgagcca gcaagtcatt attttcgata ccacattgcg cgacggtgaa
    38161 caggcgttac aggcaagctt gagtgtgaaa gaaaaactgc aaattgcgct ggcccttgag
    38221 cgtatgggtg ttgacgtgat ggaagtcggg ttccccgtct cttcgccggg tgattttgaa
    38281 tcagtgcaaa ccatcgctcg ccaggttaaa aacagccgtg tatgcgcgtt agctcgctgc
    38341 gtggagaaag atatcgacgt ggcggccgaa tccctgaaag tcgccgaagc cttccgtatt
    38401 cataccttta ttgccacttc gccaatgcac atcgccacca agctgcgcag cacgctggac
    38461 gaggtgatcg aacgcgctat ctatatggtg aaacgcgccc gtaattacac cgatgatgtt
    38521 gaattttctt gcgaagatgc cgggcgtaca cccattgccg atctggcgcg agtggtcgaa
    38581 gcggcgatta atgccggtgc caccaccatc aacattccgg acaccgtggg ctacaccatg
    38641 ccgtttgagt tcgccggaat cattagcggc ctgtatgaac gcgtgcctaa catcgacaaa
    38701 gccattattt ccgtacatac ccacgacgat ttgggcctgg cggtcggcaa ctcactggcg
    38761 gcggtacatg ccggtgcacg ccaggtggaa ggtgcaatga acgggatcgg cgagcgtgcc
    38821 ggtaactgtt ccctggaaga agtcatcatg gcgattaaag ttcgtaagga tattctcaac
    38881 gtccacaccg ccattaatca ccaggagata tggcgcacca gccagttagt tagccagatt
    38941 tgtaatatgc cgatcccggc aaacaaagcc attgttggca gcggcgcatt cgcacactcc
    39001 tccggtatcc accaggatgg cgtgctgaaa aaccgcgaaa actacgaaat catgacacca
    39061 gaatctattg gtctgaacca aatccagctg aatctgacct ctcgttcggg gcgtgcggcg
    39121 gtgaaacatc gcatggatga gatggggtat aaagaaagtg aatataattt agacaatttg
    39181 tacgacgcct tcctcaagct ggcggacaaa aaaggtcagg tgtttgatta cgatctggag
    39241 gcgctggcct tcatcggtaa gcagcaagaa gagccggagc atttccgtct ggattacttc
    39301 agcgtgcagt ctggctctaa cgatatcgcc accgccgccg tcaaactggc ctgcggcgaa
    39361 gaagtcaaag cagaagccgc caacggtaac ggtccggtcg atgccgtcta tcaggcgatt
    39421 aaccgcatca ctgactataa cgtcgaactg gtgaaataca gcctgaccgc caaaggtcac
    39481 ggtaaagatg cgctgggtca ggtggatatt gtcgccaact acaacggtcg ccgcttccac
    39541 ggcgtcggcc tggccaccga tattgtcgag tcctccgcca aagccatggt gcacgtactt
    39601 aacaatatct ggcgtgccac agaagtcgaa aaagagttgc aacgcaaagc tcaacacaac
    39661 gaaaacaaca aggaaaccgt gtgatgtcga agaattacca tattgccgta ttgccggggg
    39721 atggcattgg tccggaagtg atgacccagg cgctgaaagt gctggatgcc gtgcgcaacc
    39781 gctttgcgat gcgcatcact accagccatt acgatgtagg cggcgcagcc attgataacc
    39841 acgggcaacc actgccgcct gcgacggttg aaggttgtga gcaagccgat gccgtgctgt
    39901 ttggctcggt aggcggcccg aaatgggaac atttaccacc ggaccagcaa ccagaacgcg
    39961 gcgcgctgtt gcctttgcgt aagcacttca aattattcag caacctgcgt ccggcaaaac
    40021 tgtatcaggg gctggaagca ttctgtccgc tgcgtgcaga cattgccgca aacggcttcg
    40081 acatcctgtg cgtgcgcgaa ctgaccggcg gcatctattt cggtcagcca aaaggccgcg
    40141 aaggtagcgg acaatatgaa aaagcgtttg ataccgaggt gtatcaccgt tttgagatcg
    40201 agcgtattgc ccgcatcgcg tttgaatctg ctcgtaaacg tcgccataaa gtgacgtcga
    40261 tcgataaagc caacgtgctg caatcctcta ttttatggcg tgaaatcgtc aacgagatcg
    40321 ccacggaata cccggatatc gaactggcgc atatgtacat cgacaacgcc accatgcagc
    40381 tgattaaaga tccatcacag tttgacgttc tgctgtgctc caacctgttt ggcgacattc
    40441 tgtctgacga gtgcgcaatg atcactggct cgatggggat gttgccttcc gccagcctga
    40501 acgagcaagg ttttggactg tatgaaccgg cgggcggctc ggcaccagat atcgcaggta
    40561 aaaacatcgc caacccgatt gcgcaaattc tgtcgctggc actgctgctg cgttacagcc
    40621 tggatgccga tgatgcggct tccgccattg aacgcgccat taaccgcgca ttagaagaag
    40681 gcattcgcac cggggattta gcccgtggcg ctgccgccgt tagtaccgat gaaatgggcg
    40741 atatcattgc ccgctatgtg gcagaagggg tgtaatcatg gctaagacgt tatacgaaaa
    40801 attattcgac gctcacgtag tgtacgaagc cgaaaatgaa accccgctgt tatatatcga
    40861 ccgccacctg gtgcatgaag tgacctcacc gcaggcgttc gatggtctgc gcgcccacgg
    40921 tcgcccggta cgtcagccgg gcaaaacctt cgccaccatg gatcacaacg tctctaccca
    40981 gactaaagac attaatgcct gcggtgaaat ggcgcgcatc cagatgcagg agctgatcaa
    41041 aaactgcaaa gaatttggcg tcgaactgta tgacctgaat cacccctatc aagggatcgt
    41101 ccacgtaatg gggccggaac agggcgtgac cttgccgggg atgaccattg tctgcggcga
    41161 ctcgcatacc gccacccacg gcgcgtttgg cgcactggcc tttggtatcg gcacttccga
    41221 agttgaacac gtactggcaa cgcaaaccct gaaacagggc cgtgcgaaga ccatgaaaat
    41281 tgaagtccag ggcaaagccg cgccgggcat taccgcaaaa gatatcgtgt tggcaattat
    41341 cggtaaaacc ggtagcgcag gcggcaccgg gcatgtggtg gagttttgcg gcgaagcaat
    41401 ccgtgattta agcatggaag gtcggatgac cctgtgcaat atggcaatcg aaatgggcgc
    41461 gaaagccggt ctggttgcac ctgacgaaac cacctttaac tatgtcaaag gccgtctgca
    41521 tgcgccgaaa ggcaaagatt tcgacgacgc cgttgcctac tggaaaaccc tgcaaaccga
    41581 cgaaggcgca actttcgata ccgttgtcac tttgcaggca gaagagattt caccgcaggt
    41641 cacctgggga actaacccag gccaggtgat ttccgtgaac gacaatattc ccgatccggc
    41701 ttcgtttgcc gatccggttg aacgcgcgtc ggcagaaaaa gcgttggcct atatggggct
    41761 gaaaccgggt attctgctga ccgaagtggc tatcgacaaa gtgtttatcg gttcctgcac
    41821 caactcacgt attgaagatt tacgcgcggc ggcggaaatc gccaaagggc ggaaagtcgc
    41881 gccaggcgta caggcgctgg tggttcccgg ctctggtccg gtaaaagcgc aggcggaagc
    41941 agaaggtctg gataaaatct ttattgaagc cggttttgaa tggcgcttgc ctggctgctc
    42001 aatgtgtctg gcgatgaaca acgaccgtct gaatccgggc gaacgttgtg cctccaccag
    42061 caaccgtaac tttgaaggcc gtcaggggcg cggcgggcgc acgcatctgg tcagcccggc
    42121 aatggctgcc gccgcggctg tgaccggcca tttcgccgac attcgcaaca ttaaataagg
    42181 agcacaccat ggcagagaaa tttatcaaac acacaggcct ggtggttccg ctggatgccg
    42241 ccaatgtcga taccgatgca atcatcccga aacagttttt gcagaaagtg acccgtactg
    42301 gttttggcgc gcatctgttt aacgactggc gttttctgga tgaaaaaggc caacagccaa
    42361 acccggactt cgtgctgaac ttctcgcagt atcagggcgc ttccattttg ctggcacgag
    42421 aaaacttcgg ctgtggctcc tcgcgtgagc acgcgccctg ggcattgacc gactacggtt
    42481 ttaaggtggt gattgcgccg agttttgctg acatcttcta cggcaatagc tttaacaacc
    42541 agctgctgcc ggtgaaatta agcgacgcag aagtggatga gctgtttgcg ctggtgaaag
    42601 ccaatccggg gattcatttt gatgtagacc tggaagcgca agaggtgaaa gcgggtgaga
    42661 aaacctatcg ctttaccatt gatgccttcc gccgccactg catgatgaac ggtctggaca
    42721 gcattgggct taccttgcag cacgacgacg ccattgcggc ttatgaagcg aagcaacccg
    42781 cgtttatgcg ttagtttgta caccgtccga tactcaatcg ccaaaaagaa aaaagccagc
    42841 agataatcac tgctggcgtg gcgattaaca ccaatactca gtcacacatg atgtccgcaa
    42901 atcagtagtc agaatgactc ccctcccact gcatcagttc ttcaagcatg gttaatcgtt
    42961 gtgggacgct taaccaacgc aaccagcaca acttcgctgt cgcggtaaaa tagtgctgat
    43021 aaaactgacg aatggggcac ctccttgctt catcgttagg aattattgac ttaatatagg
    43081 gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt tatgccatct gctcgtctgc
    43141 aacaacagtt catccgcctg tggcaatgct gcgagggtaa atcgcaggac acaacgctca
    43201 acgaactggc agcgttattg agctgctcgc gtcgtcatat gcgtaccctg ctcaacacca
    43261 tgcaggatcg cggctggctg acgtgggaag cggaagtcgg gcgtggtaaa cgctcgcgtc
    43321 tgacattcct ctataccggg ctggcgcttc agcaacagcg ggcggaagac ctgctggagc
    43381 aggaccgtat cgaccaacta gtgcagttag ttggcgacaa agcgactgtg aggcaaatgc
    43441 tggtttctca tctgggccgc agcttccgcc aggggcggca catcctgcgc gtgctctact
    43501 atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg ccgttccgaa acccatatcg
    43561 cccggcaaat cttcagttcg ctaacgcgca taaatgagga aaatggggaa ctggaagcag
    43621 acatcgccca ccactggcag caaatatcac cgcttcactg gcgtttcttt ttgcgtccag
    43681 gagtccattt tcatcatggt cgtgaactgg aaatggacga cgtgatcgcc tctttaaaac
    43741 gaatcaatac gctgccgctc tattcgcata ttgctgacat tgtgtcgccg acgccctgga
    43801 cgctggatat ccacctcacg cagccggatc gctggttgcc gttactgctg ggacaagttc
    43861 cggcgatgat cctgccgcgc gaatgggaaa ccctcagtaa ctttgccagc catcccatcg
    43921 gcaccggtcc gtatgcggtg attcgcaaca gcaccaatca actgaaaatt caggcattcg
    43981 atgacttctt cggttaccgg gcattaatcg atgaagttaa cgtctgggtt ctgccggaaa
    44041 ttgccgacga gccagccgga gggctgatgc taaaaggtcc acagggcgag gaaaaagaga
    44101 ttgaaagccg cctggaggaa ggttgctact atttactgtt cgatagccgc acccatcgcg
    44161 gggcgaatca gcaagtcagg gactgggtaa gctatgtgct ttctccaact aatctggtct
    44221 atttcgctga ggaacagtac cagcaactgt ggttcccggc ttatggactg ctcccccgtt
    44281 ggcatcatgc ccgcaccata acgagcgaaa aaccggctgg tctggaaagc ctcaccctga
    44341 ccttttatca ggatcacagt gagcatcggg tgattgccgg gatcatgcag cagattctgg
    44401 caagccacca ggtcacactg gaaatcaaag agatcagcta cgatcagtgg catgaaggag
    44461 agatcgagag cgatatctgg cttaacagcg ccaactttac gctgccgctg gatttttcgc
    44521 tgttcgcgca cctgtgcgag gtaccgctgc tccaacactg tattccaatc gactggcaag
    44581 tcgacgccgc ccgctggcgc aatggcgaaa tgaacctggc gaactggtgc caacaactgg
    44641 tcgccagcaa agcaatggtg ccacttatcc accactggct gatcattcag ggacaacgca
    44701 gtatgcgcgg cctgcgcatg aacaccctcg gctggtttga ttttaaatca gcgtggtttg
    44761 cgccgccgga tccagagtag cttgcgcctt ttggcggtca tgagcgcatt ttttatattc
    44821 agacggaatg ataaaataca ttacttttat attttctctg ttttatgcat cgtctgatta
    44881 gcgtcgcaaa taagattaaa gaaggggcat tccagcccct ctttcatatt ctgagttaat
    44941 caataacggc gcagtctagc cttgttctgg ccggaaaggt cagctttttc aggttaacct
    45001 cgcgcaggtc agtcatacca acggctattg ttcgtttcag cattgctcag gtcaacctcg
    45061 agacagtcaa taccacaaat gacaaccccg ttaaagtaag cattactcag atcaatcccc
    45121 ccggtcctgg tgctcttttt cagctgcaac tccagagtaa atatcgcctc atcgccaata
    45181 aaaaaactga cgcataacgt accttctggc gagagtgagg ccttcgcggc catattttcc
    45241 ggatcttttg cgtattcttt gagttcatta aattttatca gccagtgagt taacttatca
    45301 cgcgccttcc cgtcgacagc cccggtaaag tattttgagt agtcatcact attctggaaa
    45361 taaaatgcat ccttcagaat ttgccttgcc gcagtctggg aatgtttatc tgactggaag
    45421 agattgcgta acgtctccca tagctcttca aagatattca tattctcggt ctttttgacc
    45481 aagttagcca tcctgataat ctgagaatta gtaaggcctg gatctttagt ggtattacta
    45541 gcagggatac taatctgttc tgggtttctt actttcatgc tgatagctcc actctgaata
    45601 aaaaactcag aataagcgtc ctgaataaga atactgtcaa aaatcgctca ttcacgcaat
    45661 aaacggttac gttacctatt ttgactgtag cgaagggatt ctttcggatc aacgctctcc
    45721 agctatgcaa atacgctctt gatagctgaa cgaatattgg cttttttaat acgccttctc
    45781 tatggattgc tggttgataa caaaatcact acactaacgc cgttctcaac ggggtgccac
    45841 gcggacgcgt gcgctgagaa aatacccgtc gaacctgatc cggataacgc cggcgaaggg
    45901 atttgaggct ccttctcaag tcctttgcca ctcttttttg aggtgcaaag tgttaaaaaa
    45961 atgtcttccc ctgctgttgc tgtgcacagc gcccgttttc gctaaacccg ttctgactgt
    46021 ttatacctac gattccttcg ccgccgactg ggggcctggt ccggtggtta aaaaagcctt
    46081 tgaagccgac tgtaattgcg aactgaaact ggtggcgctg gaagatggcg tttcgcttct
    46141 caaccgtcta cggatggaag gcaaaaacag taaagccgat gtggtgctgg ggctggataa
    46201 caatctgtta gacgccgcca gtaaaaccgg actgtttgcc aaaagcggtg tggcagcgga
    46261 tgccgttaac gttcccggcg gctggaataa tgacattttc gtaccgtttg attatggcta
    46321 cttcgccttc gtttatgaca agaacaaact gaaaaacccg ccacaaagcc tgaaagaact
    46381 ggttgagagc gatcaaaact ggcgggtgat ttatcaggat ccgcgcacca gtacaccggg
    46441 gctgggtctg ttgctatgga tgcaaaaagt ctatggcgat gacgccccac aagcctggca
    46501 gaaactggcg aagaaaacgg tcacggtcac caaaggctgg agcgaagcct acggcctgtt
    46561 tttaaaaggt gaaagcgatc tggtactgag ttacaccacc tctccggctt atcacattct
    46621 cgaagagaag aaagataact acgccgccgc gaacttcagc gaaggtcact atctgcaagt
    46681 ggaagtcgcc gctcgcaccg ctgccagcaa gcagccggag ctggcgcaaa aattcctcca
    46741 gtttatggtt tctccggctt tccagaatgc gatcccaacc ggcaactgga tgtatccggt
    46801 ggcaaacgtc acgctgcctg ccggttttga aaaattgacc aaacccgcaa ccacgctgga
    46861 gttcacgcca gccgaagtgg cggcacaacg tcaggcatgg attagcgaat ggcaacgcgc
    46921 cgtcagccgt taattcccgg ctggttaatt ccaggtgtaa gcgccgccac gctggtggta
    46981 gcggttgcgc tggcggcgtt tctcgccctg tggtggaacg cgccgcaggg tgactggtcg
    47041 gcagtctggc aggacagcta cctgtggcat gtggtgcgct tctccttctg gcaggcgttt
    47101 ctctcggcgc tactctctgt cgtacccgcg atattcctcg cccgcgcgct ctatcgcagg
    47161 cgctttccgg gtcggctggc gctgttgcgt ctgtgcgcaa tgaccttgat cctcccggtg
    47221 ctggttgctg ttttcggcat tcttagcgtc tatggtcgcc agggctggct ggcatcgctc
    47281 tgccaatcgc tcggtctgga gtggaccttt tcgccctacg gcctgcaagg cattttgctg
    47341 gcccatgtgt tttttaatct gccgatggcg agccgcttat tactccaggc actggaaaac
    47401 attcccggcg aacaacgtca gcttgccgcc cagcttggga tgcgcggctg gcattttttc
    47461 cgcttcgtcg aatggccgtg gttacggcga caaatcccgc cggttaccgc gcttatcttt
    47521 atgctctgtt tcgccagctt cgccaccgtg ttatcgctgg gcggcggtcc gcaggcaacc
    47581 actatcgagc tggcaatcta tcaggcgctg agttacgact acgatcctgc ccgcgcggcg
    47641 atgctggcgc tgatccagat ggtgtgttgc cttgggctgg tgctgttgag tcagcgactg
    47701 agtaaggcca ttgcgcccgg caccacgctg ctgcaaggct ggcgcgaccc ggacgatcgt
    47761 ctgcatagcc gcatttgcga cacgatgtta attgtgctgg cgctgctgct gttgctgcca
    47821 ccgttgctgg cggtgatcgt cgatggggta aatcgccagt tgccggaagt gctggcacaa
    47881 ccggtgctgt ggcaggcgct gtggacctcg ttgcgtattg cgctggcggc aggtgtattg
    47941 tgcgtagtgc tgaccatgat gctgctatgg agcagtcgcg aactgcgggc gcggcaaaaa
    48001 atgctggcgg gccaggcgct ggagatgagc ggcatgttga tcctcgctat gccggggatt
    48061 gtgctggcaa cgggcttctt tttactgctc aacaacacta tcggcctgcc acaatctgct
    48121 gacggcattg tgattttcac caatgcgtta atggcgatcc cctacgccct gaaagtgctg
    48181 gaaaacccga tgcgcgatat caccgcccgc tacagcatgt tatgtcagtc gctggggatt
    48241 gaaggctggt cacgcttaaa agtagtggag ctgcgcgccc tgaaacgccc gctggcgcag
    48301 gcgctggcct ttgcctgcgt gctgtcgatt ggtgattttg gcgtggtggc gttgttcggt
    48361 aacgatgatt tccgcaccct gccgttttat ctctaccagc aaattggctc ctatcgcagc
    48421 caggacggcg cggtcaccgc gttaattctg ctgctgctct gttttctgct gtttagcgtg
    48481 attgaaaaaa taccggggcg aaatgttaaa actgactgat atcacctggc tttatcacca
    48541 tttgccgatg cgttttagct taacggtaga acgcggcgag caggtggcga tcctcgggcc
    48601 aagcggcgcg ggtaaaagta ccctgctgaa cttgatcgcc ggttttctga caccagccag
    48661 cggtgcgctg actatcgacg gcgttgatca caccacgacg ccgccgtcac gccgtccggt
    48721 gtcgatgctg tttcaggaga acaacctgtt cagccacctg acggtcgcgc agaacatcgg
    48781 gctggggctt aatccaggat tgaagctgaa cgcggtacag caggagaaaa tgcacgcgat
    48841 tgcccgtcag atggggattg ataatttaat ggcgcggtta ccgggcgagc tttccggcgg
    48901 tcagcgacag cgcgtggcgt tagcgcgttg tctggtacgc gaacagccga ttttattgct
    48961 cgatgaaccg ttctctgcgc tcgatccggc gctacgtcag gagatgttga cgctggtgag
    49021 cactagctgc cagcagcaaa aaatgacgct attgatggtg tcgcacagtg tggaagatgc
    49081 ggcgcggatt gccacgcgct cggtagtagt cgccgacggg cgaatcgcct ggcagggtat
    49141 gaccaatgag ttgttgagcg gtaaggcgag tgcttcggcg ctgttgggaa ttacaggtta
    49201 atatgcactg tcggatgcgg cgtgaacgcc ttatccgacc tacgggtgcg ttttgtccgc
    49261 atcaagcgcc ccacacaaga ctatgcccta aactccaacc actttacgca aaatatcgat
    49321 atacaccggc attaacggat ggcgaactaa caccaccagc gccaccacgc cgatggcaga
    49381 aatcaacggc gtcagccaca acaaacgacc gcgggacaaa taatgactca aacgatcggt
    49441 tgctttgccg ctgcgccata accgccagca cagccagcca ccaacccaca aaaataccgc
    49501 cgtcgccagc agcaaccatt taaactcacc gctctgcatt ccggctggaa tatcgatcgc
    49561 cgcacccgcc agaatccccg gcaggaagta aaacggtggc cacagcaggc agccgataat
    49621 attcggcgtg ataaattttg ccaccgggag atccagcatt cccgccacca ttggcaccag
    49681 cggacgcgtc gggccaacaa aacgaccgac cagaatggtg aacatgctgt gttgatgcaa
    49741 cgcatgttca gttttatcga gtaatgcttt gttcttcttc agaaatgacc aacgatgcaa
    49801 tggcttttta aaacgccagc ccagccagaa ggagatccag tcgcccagca gacaaccgac
    49861 gatccctgcc agccaggcgt gccagaaact taactcgccg ctgccaatca gcgctcccag
    49921 ccccgccatc agcaccgtac cgggtagaat caaaccgacc agcgccagcg actccagaaa
    49981 ggccaccaac accaccgcca tcaatgaata cacggtggat tgggtaataa agtgttccag
    50041 caatgcttgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
    50101 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
    50161 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
    50221 taaatactcg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
    50281 taggcatccg ggtagtgctc aaaagcagct tcgcctggct gatgcgttgg tcctcgcgcc
    50341 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
    50401 ggcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
    50461 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
    50521 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
    50581 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
    50641 gcgcttcatc cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt
    50701 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
    50761 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
    50821 agacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
    50881 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataacca ttggcctcaa
    50941 tcggcgttaa acccgccacc agatgggcgt taaacgagta tcccggcagc aggggatcat
    51001 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
    51061 attgcatcag atattgccgt cactgcgtct tttactggct cttctcgcta acccaaccgg
    51121 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
    51181 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgataatttg cacggcgtca
    51241 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
    51301 tatcgcaact ctctactgtt tctccatacc cgtttttttg gatggagtga aacgatggcg
    51361 attgcaattg gcctcgattt tggcagtgat tctgtgcgag ctttggcggt ggactgcacc
    51421 accggtgaag aaatcgccac cagcgtagag tggtatcccc gttggcaaaa agggcaattt
    51481 tgtgatgccc cgaataacca gttccgtcat catccgcgtg actacattga gtcaatggaa
    51541 gcggcactga aaaccgtgct tgcagaactt agcgtcgaac agcgcgcagc tgtggtcggg
    51601 attggcgttg acagtaccgg ctcgacgccc gcaccgattg atgccgacgg taacgtgctg
    51661 gcgctgcgcc cggagtttgc cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac
    51721 actgcggttg aagaagcgga agagattacc cgtttgtgcc acgcgccggg caatgttgac
    51781 tactcccgct atattggcgg tatttattcc agcgaatggt tctgggcaaa aatcctgcat
    51841 gtgactcgcc aggacagcgc cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac
    51901 tgggtgccag ctctgctttc cggtaccacg cgcccgcagg atattcgtcg cggacgttgc
    51961 agcgccgggc ataagtcgct atggcacgaa agctggggcg gcctgccgcc agccagtttc
    52021 tttgatgagc tggatccgat cctcaatcgc catttgcctt ccccgctgtt cactgatacc
    52081 tggactgccg atattccggt gggcacctta tgcccggaat gggcgcagcg tctcggcctg
    52141 cctgaaagcg tggtgatttc cggcggcgcg tttgactgcc atatgggcgc agttggtgca
    52201 ggcgcacagc ctaacgcact ggtaaaagtt atcggtactt ccacctgcga cattctgatt
    52261 gccgacaaac agagcgttgg cgagcgggca gtgaaaggta tttgcggtca ggttgatggc
    52321 agcgtggtgc ctggatttat cggtctggaa gcaggccaat cggcgtttgg tgatatctac
    52381 gcctggttcg gtcgcgtact cggctggccg ctggaacagc ttgccgccca gcatccggaa
    52441 ctaaaagagc aaatcgacgc cagccagaaa caactgcttc cggcgctgac cgaagcatgg
    52501 gccaaaaatc cgtctctgga tcacctgccg gtggtgctcg actggtttaa cggccgccgc
    52561 acaccgaacg ctaaccaacg cctgaaaggg gtgattaccg atcttaacct cgctaccgac
    52621 gctccgctgc tgttcggcgg tttgattgct gccaccgcct ttggcgcacg cgcaatcatg
    52681 gagtgcttta ccgatcaggg gatcgccgtc aataacgtga tggcgctggg cggcatcgcg
    52741 cggaaaaacc aggtcattat gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt
    52801 gttgcctctg accaatgctg tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa
    52861 gtgcacgcag acatcccatc agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg
    52921 caaccgcgca gcgaacaggc acaacgcttt gaacagcttt atcgccgcta tcagcaatgg
    52981 gcgatgagcg ccgaacaaca ctatcttcca acttccgccc cggcacaggc tgcccaggcc
    53041 gttccgactc tataaggaca cgataatgac gatttttgat aattatgaag tgtggtttgt
    53101 aattggcagc cagcatctgt atggcccgga aaccctgcgt caggtcaccc aacatgccga
    53161 gcacgttgtg aaagcgctga atacggaagc gaaactgccc tgcaaactgg tactgaaacc
    53221 gctgggcacc acgccggatg aaatcaccgc tatttgccgt gatgctaatt acgacgatcg
    53281 ttgcgctggt ctggtggtgt ggctacacac cttctccccg gccaaaatgt ggatcaacgg
    53341 cctgaccatg ctcaacaaac cgttgctgca attccacacc cagttcaacg cggcgctgcc
    53401 gtgggacagc atcgatatgg actttatgaa cctgaaccag accgcgcacg gcggtcgcga
    53461 gttcggcttc attggcgcgc gtatgcgtca gcaacatgcc gtggttaccg gtcactggca
    53521 ggataaacaa gcccatgagc gtatcggctc ctggatgcgt caggcggtat ctaaacagga
    53581 tacccgtcat ctgaaagtct gccgatttgg cgataacatg cgtgaagtgg cggtcaccga
    53641 tggcgataaa gttgccgcac agatcaagtt cggtttctcc gtcaatacct gggcggttgg
    53701 cgatctggtg caggtggtga actccatcag cgacggcgat gttaacgcgc tggtcgatga
    53761 gtacgaaagc tgctacacca tgacgcctgc cacacaaatc cacggcgaaa aacgacagaa
    53821 cgtgctggaa gcggcgcgta ttgagctggg gatgaaacgt ttcctggaac aaggtggctt
    53881 ccacgcgttc accaccacct ttgaagattt gcacggtctg aaacagcttc ctggtctggc
    53941 cgtacagcgt ctgatgcagc agggttacgg ctttgcgggc gaaggcgact ggaaaactgc
    54001 cgccctgctt cgcatcatga aggtgatgtc aaccggtctg cagggcggca cctcctttat
    54061 ggaggactac acctatcact tcgagaaagg caatgacctg gtgctcggct cccatatgct
    54121 ggaagtctgc ccgtcgatcg ccgcagaaga gaaaccgatc ctcgacgttc agcatctcgg
    54181 tattggtggt aaggacgatc ctgcccgcct gatcttcaat acccaaaccg gtccagcgat
    54241 tgtcgccagc ttgattgatc tcggcgatcg ttaccgtcta ctggttaact gcatcgacac
    54301 ggtgaaaaca ccgcactccc tgccgaaact gccggtggcg aatgcgctgt ggaaagcgca
    54361 accggatctg ccaactgctt ccgaagcgtg gatcctcgct ggtggcgcgc accataccgt
    54421 cttcagccat gcgctgaacc tcaacgatat gcgccagttc gccgagatgc acgacattga
    54481 aatcacggtg attgataacg acacccgcct gccagcgttt aaagacgcgc tgcgctggaa
    54541 cgaagtgtat tacgggtttc gccgctaagt agccgcatcc ggtatgtaac gcctgatgcg
    54601 acgctggcgc gtcttatcag gcctacacgc tgcaattttg taggccggat aagcaacgcg
    54661 catccggcat tcagcgcctg atgcgacgct gacgcgtctt atctggccta cacgctgcga
    54721 ttttgtaggc cggataagca acgcgcatcc ggcacgaagg agtcaacatg ttagaagatc
    54781 tcaaacgtca ggtattagaa gccaacctgg cgctgccaaa acacaacctg gtcacgctca
    54841 catggggcaa cgtcagcgcc gttgatcgcg agcgcggcgt ctttgtgatc aaaccttccg
    54901 gcgtcgatta cagcatcatg accgctgacg atatggtcgt ggtcagcatc gaaaccggtg
    54961 aagtggttga aggtgcgaaa aagccctcct ccgatacgcc aactcaccga ctactctatc
    55021 aggcattccc gtccattggc ggcattgtgc acacgcactc gcgccacgcc accatctggg
    55081 cgcaggcggg tcagtcgatt ccagcaaccg gcaccaccca cgccgactat ttctacggca
    55141 ccattccctg cacccgcaaa atgaccgacg cagaaatcaa cggcgaatat gagtgggaaa
    55201 ccggtaacgt catcgtagaa accttcgaaa aacagggtat cgatgcagcg caaatgcccg
    55261 gcgttctggt ccattctcac ggcccatttg catggggcaa aaatgccgaa gatgcggtgc
    55321 ataacgccat cgtgctggaa gaggtcgctt atatggggat attctgccgt cagttagcgc
    55381 cgcagttacc ggatatgcag caaacgctgc tggataaaca ctatctgcgt aagcatggcg
    55441 cgaaggcata ttacgggcag taatgactgt ataaaaccac agccaatcaa atgaaaccag
    55501 gctataatca agcctggttt tttgatggat tttcagcgtg gcgcaggcag gttttatctt
    55561 aacccgacac tggcgggata ccccgcaagg aacagaggtc tccttctggc tggcgacgga
    55621 caacgggccg ttgcaggtta cgcttgcacc gcaagagtcc gtggcgttta ttcccgccga
    55681 tcaggttccc cgcgctcagc atattttgcg gggtgaacaa ggctttcgcc tgacgccgct
    55741 ggcgttaaag gattttcacc gccagccggt gtatggcctt tactgtcgcg cccatcgcca
    55801 gttgatgaat tacgaaaagc gcctgcgtga aggtggcgtt accgtctacg aggccgatgt
    55861 gcgcccgcca gaacgctatc tgatggagcg gtttatcacc tcaccggtgt gggtcgaggg
    55921 tgatatgcac aatggcgcta tcgttaatgc ccgtctgaaa ccgcatcccg actatcgtcc
    55981 gccgctcaag tgggtttcta ttgatattga aaccacccgc cacggagagc tgtactgcat
    56041 cggcctggag ggctgcgggc agcgcatcgt ttatatgctg ggaccagaga atggcgacgc
    56101 ctccgcgctc gattttgaac tggagtacgt cgccagccgc ccgcagctac tggaaaaact
    56161 caacgcctgg tttgcaaact acgatcctga tgtgatcatc ggttggaacg tggtgcagtt
    56221 tgatctgcga atgctgcaaa aacatgccga gcgttatcgt attccgctgc ggctggggcg
    56281 cgataacagt gagctggagt ggcgcgagca cggctttaaa aacggcgtct tttttgccca
    56341 ggctaaaggt cggctaatta tcgacggtat cgaggcgctg aaatccgcgt tctggaattt
    56401 ctcttcattc tcgctggaaa ctgtcgctca ggagctatta ggcgaaggaa aatctatcga
    56461 taacccgtgg gatcgaatgg acgaaattga ccgccgtttc gccgaagata aacctgcgct
    56521 ggcaacttat aacctgaaag attgcgagct ggtgacgcag atcttccata aaactgaaat
    56581 catgccgttt ttgctcgaac gagcaacggt gaacggcctg ccggtggatc gacacggcgg
    56641 gtcggtggca gcgtttggtc atctctattt tccccgtatg catcgcgctg gttatgtcgc
    56701 gcctaatctc ggcgaagtgc cgccgcacgc cagccctggc ggctacgtga tggattcgcg
    56761 acctgggctt tatgattcgg tgctggtgct ggactacaaa agcctgtacc cgtcgatcat
    56821 ccgcaccttt ctgattgatc ccgtcgggct ggtggaaggc atggcgcagc ctgatccaga
    56881 gcacagcacc gaaggctttc tcgatgcctg gttctcgcga gaaaaacatt gcctgccgga
    56941 gattgtgact aacatctggc acggacgcga tgaagccaaa cgccagggta acaaaccgct
    57001 gtcgcaggcg ctgaaaatca tcatgaatgc cttttatggc gtgctcggca ccaccgcctg
    57061 ccgcttcttc gatccgcggc tggtatcgtc gatcaccatg cgtggtcatc agatcatgcg
    57121 gcaaaccaaa gcgttgattg aagcgcaggg ctacgacgtg atctacggcg ataccgactc
    57181 aacgtttgtc tggctgaaag gcgcacattc ggaagaagaa gcgacgaaaa tcggtcgtgc
    57241 actggtgcag cacgttaacg tctggtgggc agaaacgctg caaaaacaac agctgaccag
    57301 cgcactggaa ctggagtatg aaacccattt ctgccgtttt ctgatgccaa ccattcgcgg
    57361 agccgatacc ggcagtaaaa agcgttatgc cgggctgatt caggagggcg acaaacagcg
    57421 gatggtgttt aaagggctgg aaaccgtgcg tacggactgg acgccgctgg cccagcagtt
    57481 tcagcaggag ctttacctgc gtatcttccg caacgagcca tatcaggagt acttccgcga
    57541 aaccatcgac aaactgatgg cgggtgaact ggatgcgcgg ctggtttacc gtaaacgcct
    57601 tcgccgtccg ctgagcgaat atcagcgtaa tgttccgcct catgtacgcg ccgctcgtct
    57661 tgccgatgaa gaaaaccaaa agcgcggtcg ccccttgcaa tatcagaacc gcggcaccat
    57721 taagtacgta tggaccacca acggcccgga gccgctggac taccaacgtt caccactgga
    57781 ttacgaacac tatctgaccc gccagctaca acccgtggcg gagggaatac tcccttttat
    57841 tgaggataat tttgctacac ttatgaccgg gcaacttggg ctattttgag caaaaaaaga
    57901 gttcgccaga taccattttg atgcgtgacg aatgctttgc catccagtac catagcgccc
    57961 tttccattcc tggacctgaa taacaccact acctcataag cacggtagcg ggtggttatt
    58021 gcctgcaatt aaagatatag agccgaacac atatgccttt tacacttggt caacgctgga
    58081 tcagcgatac agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat gcgcgaactg
    58141 tcactttact tttcccatct actggtgaaa accgtctgta cgcacgcagt gattcccccg
    58201 tgacccgcgt gatgttcaac cctggtgata ccattaccag ccatgacggc tggcagatgc
    58261 aagtcgaaga agtaaaagaa gaaaatggct tgctgaccta tatcggtact cgcctggata
    58321 ctgaagagtc cggcgtagcc ctgcgtgaag ttttccttga tagcaaactg gtgttcagca
    58381 aaccgcagga ccgcctgttt gccgggcaga ttgaccgtat ggaccgcttt gcgctgcgtt
    58441 atcgcgcgcg taagtattcc agcgaacagt tccgtatgcc gtacagcggc ctgcgcggtc
    58501 agcgtaccag cctgatcccg caccagctca acatcgctca tgatgttggc cgccgccacg
    58561 cgccgcgcgt cctgctggct gacgaagtgg gtttagggaa aaccattgaa gccgggatga
    58621 tccttcatca gcaactgctc tctggcgctg ctgaacgtgt gctgattatc gtcccggaaa
    58681 ccttacagca tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc tttgcgctgt
    58741 ttgatgatga gcgttatgcc gaagctcagc acgatgctta caacccgttc gacaccgaac
    58801 agctggtgat ttgctcgctg gattttgccc gccgtagcaa acagcgtctg gaacatctct
    58861 gtgaagccga gtgggacctg ctggtggtcg atgaagcgca tcacctggtg tggagcgaag
    58921 acgcgccgag ccgcgaatat caggctattg aacaactggc agagcacgtg ccgggcgttc
    58981 tgctgctgac cgcaaccccg gaacagctgg gaatggaaag ccacttcgcc cgtctgcgtc
    59041 tgctggaccc gaaccgtttc cacgatttcg cgcaattcgt tgaagagcag aaaaattatc
    59101 gtccagtagc ggacgccgtt gccatgctgc tggcaggtaa caaactgagc aatgacgaac
    59161 tgaacatgct tggcgagatg atcggcgagc aggatatcga gccgctgttg caagcagcaa
    59221 acagcgacag cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg ctgatggatc
    59281 gccacggcac cagccgcgtg ctgttccgta acacccgtaa cggtgtgaaa ggcttcccga
    59341 aacgcgagct gcacaccatt aagctgccgc taccgacgca gtatcagacg gctattaaag
    59401 tctccggcat tatgggcgca cgtaaaagtg cggaagaccg cgcccgcgat atgctctacc
    59461 cggagcgtat ttatcaggaa tttgaaggtg ataacgccac ctggtggaac ttcgatccgc
    59521 gcgttgagtg gctgatgggc tacctgacca gccatcgctc tcagaaagtg ctggtgatct
    59581 gcgctaaagc tgccactgcg ctgcaactgg agcaagtact gcgcgaacgt gaaggtattc
    59641 gcgctgcggt gttccacgaa ggtatgtcga ttatcgaacg tgaccgcgct gccgcctggt
    59701 ttgccgaaga agacaccggc gcacaggtac tgctgtgctc agaaatcggt tctgaaggac
    59761 gtaacttcca gttcgccagc cacatggtga tgtttgacct gccattcaac ccggatctac
    59821 tggagcagcg tattggtcgt ctggatcgta tcggccaagc gcacgatatt cagatccatg
    59881 tgccttatct ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac gaaggtctgg
    59941 atgcatttga gcacacctgc ccgaccggac gcactattta cgatagcgta tacaacgatc
    60001 tgattaacta tctggcttca ccggatcaaa ccgaaggctt tgacgatctg atcaaaaact
    60061 gccgcgagca acatgaagcg ctgaaagcac agctggaaca gggtcgtgac cgcctgctgg
    60121 aaatccactc caacggtggc gaaaaagccc aggcactggc agaaagcatt gaagagcagg
    60181 atgacgatac caacctgatc gccttcgcca tgaacctgtt cgatattatc ggtatcaatc
    60241 aggacgatcg cggcgacaac atgatcgtgc tgacgccgtc cgatcatatg ctggtgccgg
    60301 acttccctgg cctgtcggaa gatggcatca ccatcacctt tgatcgtgaa gtggcgctgg
    60361 cgcgtgaaga tgcgcagttt attacctggg aacatccgct gatccgcaac ggtctggatc
    60421 tgattctttc tggcgatacc ggtagcagca cgatttcact gttaaaaaac aaagcgttgc
    60481 cggtaggtac gctgttggtg gaactgattt atgtggttga agcccaggct ccgaagcagt
    60541 tgcagctcaa ccgcttcctg ccaccgacgc cggtacgtat gctgctggat aaaaacggca
    60601 acaacctggc ggcgcaggta gagtttgaaa cctttaaccg ccagcttaac gcggttaacc
    60661 gtcacaccgg cagcaaactg gttaacgccg tgcagcagga tgttcacgct atccttcaac
    60721 tgggtgaagc gcagatcgag aaatctgccc gtgcattgat tgatgcagca cgtaacgaag
    60781 ccgacgaaaa actgtctgcc gagctgtctc gtctggaagc tctgcgtgca gtgaacccga
    60841 acattcgtga cgacgaactg accgccattg agagcaaccg tcagcaggta atggaaagcc
    60901 tggatcaggc aggttggcgt ctggatgccc tgcgtttgat cgttgtaacg catcagtaac
    60961 ggagccgaaa atggggatgg aaaactacaa tccaccgcag gaaccctggt tggttatcct
    61021 gtatcaggat gaccatatta tggtggtcaa caagccgagc ggtttgttgt ctgtgccggg
    61081 tcgtctggaa gagcacaaag acagcgtgat gacgcgcatt cagcgagatt atccgcaggc
    61141 agaatcggtg catcgtctgg atatggctac cagcggcgtg attgtagtgg cgctgaccaa
    61201 agccgcggag cgggagttaa aacgccagtt ccgcgagcgc gtgccgaaaa agcagtatgt
    61261 gacccgcgtc tgggggcatc catcccccgc agaaggtctg gtggatctgc cgctgatttg
    61321 cgactggcca aaccgcccga aacagaaagt ctgttacgaa acgggtaaac ctgcgcagac
    61381 ggaatatgaa gtggtggagt atgcggcgga taacacggca agagtggtgt taaaaccgat
    61441 taccgggcgt tcgcatcaat tgcgtgtgca tatgctggcg ctggggcatc cgattctcgg
    61501 cgatcgtttt tatgcatcac cagaagcgag agcgatggca ccgcgtttgt tgctacatgc
    61561 agagatgttg acgattaccc atccggcgta tggcaatagt atgacgttta aagcgccagc
    61621 ggatttttaa cctttcctgt acgtttatac cctcatccta agggctgcct cttgtacata
    61681 aatccccgag tccggggatt ttttgtttct gccaaactgc tttcagttgt tgcgtgtggc
    61741 tacgctttcc attcggtaat tcccttcctg ctccacagcg aatttttgaa ctttttcttc
    61801 acccgttgac cgtctcttat atttcatttt atcgggtctg ttatggtcct ttctgattgt
    61861 tattcatggg ctaattagca gtctggttgg cgatgcccat ctcggtgatc cgcgtcgtac
    61921 acgacgcctt gcgtcactag cctcttctct agctcatcat gcaggtcttt ctaattgtga
    61981 aatcatccca cgctactgct aaggttgaag gggcgttcca gccccttcaa ccttagctta
    62041 ttctggctca ttcaataatt gcgttgtcag taatttgttc agcagtactt tgtttagtag
    62101 caacttcttc agtatcaatt tttttggggg tgattttatt aataataatt acctcatcat
    62161 ataggtgggt agcaccgtcc acggtagcac cgtccagggt agcaccgtcc aggttagcat
    62221 tccacccagg ttccaagtaa aggactttat tgtgaccttt cattgttttg agaccaaggt
    62281 aacaatccct catgtcaacg ccgggaaggt aagcctctct aagatcaacg cccaagttac
    62341 ctgggccttt atgcagctgc aacgtctgag tacatatctc cttattttca ataaaaaaag
    62401 tgacacttaa ctcgatatca ccgaaggtct gtgcgaccca gctcatattg ccctttgcga
    62461 ccatctcatc cggatttttt gcgcatgttt tgagttcatt gaataattcc agactatgga
    62521 taaacttttc tttctcattc tcgttgacag ccccagtaaa gtattttgag gcgttgatgt
    62581 tgccagcctt tttattcacg aaattgaaca gaagtttata agcctcagtc tgtttatctg
    62641 accagaacag atgacgtaac gtctcccaca agcgttgaaa gatattcatc ttcatgtcca
    62701 tcttgtcccc gctcaccatc ttataaatat cgttattgct catatttaaa agtgtagccg
    62761 gcatgcctgg aactgatact ttcatggtaa tagcctcgca ttgagttaaa aactcatgat
    62821 gcgcgctctg aataaatata ctttcaaaaa tcgctcattc ag
//
LOCUS       DABSYT020000018        59015 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.018, whole genome shotgun sequence.
ACCESSION   DABSYT020000018 DABSYT020000000
VERSION     DABSYT020000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 59015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.018"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..192
                     /locus_tag="ID725_RS07485"
                     /old_locus_tag="pgaptmp_001497"
     CDS             <1..192
                     /locus_tag="ID725_RS07485"
                     /old_locus_tag="pgaptmp_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850132.1"
                     /translation="KDVIEEKLNNELKLRFRMMQTILKSEVNVSPFIDQQRLNTLNPP
                     ENLRIAIEKFGWKKKTITA"
     gene            complement(249..1253)
                     /gene="argF"
                     /locus_tag="ID725_RS07490"
                     /old_locus_tag="pgaptmp_001498"
     CDS             complement(249..1253)
                     /gene="argF"
                     /locus_tag="ID725_RS07490"
                     /old_locus_tag="pgaptmp_001498"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGV7850133.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1415..1831
                     /gene="rraB"
                     /locus_tag="ID725_RS07495"
                     /old_locus_tag="pgaptmp_001499"
     CDS             1415..1831
                     /gene="rraB"
                     /locus_tag="ID725_RS07495"
                     /old_locus_tag="pgaptmp_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGV7850134.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1877..2380)
                     /gene="yjgM"
                     /locus_tag="ID725_RS07500"
                     /old_locus_tag="pgaptmp_001500"
     CDS             complement(1877..2380)
                     /gene="yjgM"
                     /locus_tag="ID725_RS07500"
                     /old_locus_tag="pgaptmp_001500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGV7850135.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            2573..3769
                     /gene="yjgN"
                     /locus_tag="ID725_RS07505"
                     /old_locus_tag="pgaptmp_001501"
     CDS             2573..3769
                     /gene="yjgN"
                     /locus_tag="ID725_RS07505"
                     /old_locus_tag="pgaptmp_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HGV7850136.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            complement(3824..6679)
                     /gene="valS"
                     /locus_tag="ID725_RS07510"
                     /old_locus_tag="pgaptmp_001502"
     CDS             complement(3824..6679)
                     /gene="valS"
                     /locus_tag="ID725_RS07510"
                     /old_locus_tag="pgaptmp_001502"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGV7850137.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6679..7122)
                     /gene="holC"
                     /locus_tag="ID725_RS07515"
                     /old_locus_tag="pgaptmp_001503"
     CDS             complement(6679..7122)
                     /gene="holC"
                     /locus_tag="ID725_RS07515"
                     /old_locus_tag="pgaptmp_001503"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGV7850138.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7476..8987)
                     /gene="pepA"
                     /locus_tag="ID725_RS07520"
                     /old_locus_tag="pgaptmp_001504"
     CDS             complement(7476..8987)
                     /gene="pepA"
                     /locus_tag="ID725_RS07520"
                     /old_locus_tag="pgaptmp_001504"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGV7850139.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9141..9191
                     /gene="ytgA"
                     /locus_tag="ID725_RS07525"
                     /old_locus_tag="pgaptmp_001505"
     CDS             9141..9191
                     /gene="ytgA"
                     /locus_tag="ID725_RS07525"
                     /old_locus_tag="pgaptmp_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGV7850140.1"
                     /translation="MSIIILATISTTQENK"
     gene            9254..10354
                     /gene="lptF"
                     /locus_tag="ID725_RS07530"
                     /old_locus_tag="pgaptmp_001506"
     CDS             9254..10354
                     /gene="lptF"
                     /locus_tag="ID725_RS07530"
                     /old_locus_tag="pgaptmp_001506"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGV7850141.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            10354..11436
                     /gene="lptG"
                     /locus_tag="ID725_RS07535"
                     /old_locus_tag="pgaptmp_001507"
     CDS             10354..11436
                     /gene="lptG"
                     /locus_tag="ID725_RS07535"
                     /old_locus_tag="pgaptmp_001507"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGV7850142.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11597..13099)
                     /gene="yjgR"
                     /locus_tag="ID725_RS07540"
                     /old_locus_tag="pgaptmp_001508"
     CDS             complement(11597..13099)
                     /gene="yjgR"
                     /locus_tag="ID725_RS07540"
                     /old_locus_tag="pgaptmp_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGV7850143.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLVRLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(13229..14248)
                     /gene="ahr"
                     /locus_tag="ID725_RS07545"
                     /old_locus_tag="pgaptmp_001509"
     CDS             complement(13229..14248)
                     /gene="ahr"
                     /locus_tag="ID725_RS07545"
                     /old_locus_tag="pgaptmp_001509"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGV7850144.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            14444..14528
                     /locus_tag="ID725_RS07550"
                     /old_locus_tag="pgaptmp_001510"
     tRNA            14444..14528
                     /locus_tag="ID725_RS07550"
                     /old_locus_tag="pgaptmp_001510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14478..14480,aa:Leu,seq:caa)
     gene            14619..16538
                     /locus_tag="ID725_RS07555"
                     /old_locus_tag="pgaptmp_001511"
     CDS             14619..16538
                     /locus_tag="ID725_RS07555"
                     /old_locus_tag="pgaptmp_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HGV7850145.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAVELI"
     gene            complement(16665..17042)
                     /locus_tag="ID725_RS07560"
                     /old_locus_tag="pgaptmp_001512"
     CDS             complement(16665..17042)
                     /locus_tag="ID725_RS07560"
                     /old_locus_tag="pgaptmp_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HGV7850146.1"
                     /translation="MNLDEEFYELQKLFAQKDLLTEKRRSSGGGFMEILLVKRQNMKI
                     KIYQEKGHQLPHIHIDYGKQAHAASYAIQSGDRIEGDLPKKYDSDVSSWLGRNRDKIL
                     EIWNALQAGAPYEPLIAELTGGV"
     gene            complement(17164..17496)
                     /locus_tag="ID725_RS07565"
                     /old_locus_tag="pgaptmp_001513"
     CDS             complement(17164..17496)
                     /locus_tag="ID725_RS07565"
                     /old_locus_tag="pgaptmp_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HGV7850147.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
     gene            complement(18500..18967)
                     /locus_tag="ID725_RS07570"
                     /old_locus_tag="pgaptmp_001514"
     CDS             complement(18500..18967)
                     /locus_tag="ID725_RS07570"
                     /old_locus_tag="pgaptmp_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HGV7850148.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            19160..19729
                     /locus_tag="ID725_RS07575"
                     /old_locus_tag="pgaptmp_001515"
     CDS             19160..19729
                     /locus_tag="ID725_RS07575"
                     /old_locus_tag="pgaptmp_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGV7850149.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            complement(19928..21244)
                     /locus_tag="ID725_RS07580"
                     /old_locus_tag="pgaptmp_001516"
     CDS             complement(19928..21244)
                     /locus_tag="ID725_RS07580"
                     /old_locus_tag="pgaptmp_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850150.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(21396..22169)
                     /locus_tag="ID725_RS07585"
                     /old_locus_tag="pgaptmp_001517"
     CDS             complement(21396..22169)
                     /locus_tag="ID725_RS07585"
                     /old_locus_tag="pgaptmp_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850151.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIVSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            22292..22750
                     /locus_tag="ID725_RS07590"
                     /old_locus_tag="pgaptmp_001518"
     CDS             22292..22750
                     /locus_tag="ID725_RS07590"
                     /old_locus_tag="pgaptmp_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7850152.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            23466..24248
                     /locus_tag="ID725_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /pseudo
     CDS             23466..24248
                     /locus_tag="ID725_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            24368..24555
                     /locus_tag="ID725_RS07600"
                     /old_locus_tag="pgaptmp_001520"
                     /pseudo
     CDS             24368..24555
                     /locus_tag="ID725_RS07600"
                     /old_locus_tag="pgaptmp_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(24753..25604)
                     /locus_tag="ID725_RS07605"
                     /old_locus_tag="pgaptmp_001521"
     CDS             complement(24753..25604)
                     /locus_tag="ID725_RS07605"
                     /old_locus_tag="pgaptmp_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HGV7850153.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALVAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(25678..25920)
                     /locus_tag="ID725_RS07610"
                     /old_locus_tag="pgaptmp_001522"
                     /pseudo
     CDS             complement(25678..25920)
                     /locus_tag="ID725_RS07610"
                     /old_locus_tag="pgaptmp_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(26145..27248)
                     /gene="zorE"
                     /locus_tag="ID725_RS07615"
                     /old_locus_tag="pgaptmp_001523"
     CDS             complement(26145..27248)
                     /gene="zorE"
                     /locus_tag="ID725_RS07615"
                     /old_locus_tag="pgaptmp_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HGV7850154.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(27252..27959)
                     /gene="zorB2"
                     /locus_tag="ID725_RS07620"
                     /old_locus_tag="pgaptmp_001524"
     CDS             complement(27252..27959)
                     /gene="zorB2"
                     /locus_tag="ID725_RS07620"
                     /old_locus_tag="pgaptmp_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HGV7850155.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            complement(27966..29618)
                     /gene="zorA2"
                     /locus_tag="ID725_RS07625"
                     /old_locus_tag="pgaptmp_001525"
     CDS             complement(27966..29618)
                     /gene="zorA2"
                     /locus_tag="ID725_RS07625"
                     /old_locus_tag="pgaptmp_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HGV7850156.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGALGILGTFIGIIIGLLNFNTESIDTSIPVLLGGLKTAFITSIVGMFFA
                     ILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLI
                     AQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDF
                     NQNLTEQFGENFKALDASVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIW
                     EECKEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGEL
                     LKSGAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNS
                     SETLTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQ
                     LSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQ
                     SLGNALLSISKGFVGNYEKLIKDYQIVMGQLQALISANKHRG"
     gene            29830..33189
                     /locus_tag="ID725_RS07630"
                     /old_locus_tag="pgaptmp_001526"
     CDS             29830..33189
                     /locus_tag="ID725_RS07630"
                     /old_locus_tag="pgaptmp_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HGV7850157.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLMLENTVDLRVSWKDGSNLKL
                     LLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLNIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAISLKNPGLHISLLKKD
                     EFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWMLPVLQTNDLLLNALCEADPEQRK
                     KNFNELIFEIDNNPLQNYCCLLTEYIKKYKMNNGLSLLDLDLFRGISSNYRVVVQLLI
                     SSCLSGDSDTIYDIQEELPFSWGWIPVSIWKDVFQKCWTYLEKQINDKTLALHILQPF
                     IAFMNHRAHIDRRLAPIANMLLTYSESLPTGCDVLPTVSREQFNEAKQMLLRNPDSFG
                     RISIFPKELWSSAITPELKSVFNKLWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQ
                     LSALFEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            33189..39503
                     /locus_tag="ID725_RS07635"
                     /old_locus_tag="pgaptmp_001527"
     CDS             33189..39503
                     /locus_tag="ID725_RS07635"
                     /old_locus_tag="pgaptmp_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGV7850158.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEEPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPATA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            39727..42585
                     /locus_tag="ID725_RS07640"
                     /old_locus_tag="pgaptmp_001528"
     CDS             39727..42585
                     /locus_tag="ID725_RS07640"
                     /old_locus_tag="pgaptmp_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGV7850159.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            42588..47522
                     /locus_tag="ID725_RS07645"
                     /old_locus_tag="pgaptmp_001529"
     CDS             42588..47522
                     /locus_tag="ID725_RS07645"
                     /old_locus_tag="pgaptmp_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850160.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            47522..53863
                     /locus_tag="ID725_RS07650"
                     /old_locus_tag="pgaptmp_001530"
     CDS             47522..53863
                     /locus_tag="ID725_RS07650"
                     /old_locus_tag="pgaptmp_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGV7850161.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            53860..55974
                     /locus_tag="ID725_RS07655"
                     /old_locus_tag="pgaptmp_001531"
     CDS             53860..55974
                     /locus_tag="ID725_RS07655"
                     /old_locus_tag="pgaptmp_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGV7850162.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
     gene            complement(56650..57345)
                     /locus_tag="ID725_RS07660"
                     /old_locus_tag="pgaptmp_001532"
                     /pseudo
     CDS             complement(56650..57345)
                     /locus_tag="ID725_RS07660"
                     /old_locus_tag="pgaptmp_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(57468..58448)
                     /gene="nanS"
                     /locus_tag="ID725_RS07665"
                     /old_locus_tag="pgaptmp_001533"
     CDS             complement(57468..58448)
                     /gene="nanS"
                     /locus_tag="ID725_RS07665"
                     /old_locus_tag="pgaptmp_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGV7850163.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            complement(58513..>59015)
                     /gene="nanM"
                     /locus_tag="ID725_RS07670"
                     /old_locus_tag="pgaptmp_001534"
     CDS             complement(58513..>59015)
                     /gene="nanM"
                     /locus_tag="ID725_RS07670"
                     /old_locus_tag="pgaptmp_001534"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGV7850164.1"
                     /translation="GESPWYGTAGAAVVNKGDKTWLINGEAKPGLRTDAVFELDFTGN
                     NLKWNRLAPVSSPDGVAGGFAGISNDSLIFAGGAGFKGSRENYQNGKNYAHEGLKKSY
                     STDIHLWHNGKWDKSGELSQGRAYGVSLPWNNSLLIIGGETAGGKAVTDSVLISVKDN
                     KVTVQN"
BASE COUNT        14941 a        13857 c        14591 g        15626 t
ORIGIN      
        1 aaagatgtta ttgaagaaaa acttaataat gaattaaaac ttaggtttag aatgatgcaa
       61 actatcttga aatcggaggt taatgtatcg ccatttattg accaacagcg tttaaataca
      121 ctaaatcctc cggaaaattt acgtatagca atagaaaaat ttggctggaa gaaaaaaact
      181 atcactgcat aaaataattt gacgccggga tgtttctgta tttcccggca tctttatagc
      241 gatatcaatt atttactgag tgtcgcgacc atcaccgctt tgatggtgtg catgcggttt
      301 tccgcctgat caaaaacaat gctggcggca gattcgaaga cctcatcagt cacttccatt
      361 ccgccatgta ggccaaactc ttccgccatt ttcttgccaa gcgtcgtttg gtcatcatgg
      421 aacgcgggca ggcagtggag gaatttgacc tccgggttac cggtcaactg catcatcttg
      481 ctgttcacct gataatcacg cagtaatgca atgcgttcag cccatttttc ttttgcttca
      541 cccatcgaca cccatacatc ggtatagata aagtcagcac cttcaacgcc tttagctaca
      601 tcttccgtca gcgtaatatt cccgccattt tgttgtgcca gggcgcggca ttccgtaacc
      661 agcgcagctt ccggccagca tgcctgtggc gcgaccagac gcaaatccag accggtaagc
      721 gccgcagctt cgagcatcga attgcccatg ttgttacgtg cgtcacctgc atagaccagc
      781 gtcatttcgt tgaatggttt accgggtaaa tgctcctgca tggtgaggag atccgccagc
      841 aactgcgtgg ggtgaaactc attggtcagg ccgttccata ccggcacgcc agcgtattcc
      901 gccagtgttt cgacaatctc ctgaccatag ccgcgatact gaataccgtc atacatgcga
      961 cccagcacgc gggcagtgtc tttaatcgac tctttatgac caatctgact gccgcttggg
     1021 ccgagataag taacgcgagc accctggtca tatgcggcaa cttcgaaaga gcatcgggta
     1081 cgggtcgagt ctttttcgaa gatgagcgcg atgtttttac cagtgagttt ggcttcttct
     1141 ttgccgcttt tcttatcggc tttcagcttc gcggctaact gcagcaggct gttgagttca
     1201 gctggcgtga aatcgagtaa tttcaggaaa tgcttatgat aaaacccgga catagatccc
     1261 tcctgtggct aacgtctcaa tgaattaaaa ttcaatttat atggatgatt attcatttgc
     1321 aagtctaaag cataaatctt tgtcacaaag gtggaggcaa tgccagtggt gtgtgacaat
     1381 atgagtatcg gcaggacatt aagaggaatg agccatggca aacccggaac aactggaaga
     1441 acagcgtgaa gaaacacgtt tgattattga agaattactg gaagatggca gcgatccgga
     1501 cgcgctgtac accatcgaac accatctttc cgcagacgat ctggaaaccc tggaaaaagc
     1561 agcagttgaa gcgtttaaac tcggttacga agtgaccgat ccagaagagc tggaagttga
     1621 agatggtgat atcgtgattt gctgcgacat cctcagcgag tgcgcgttga atgccgatct
     1681 gatcgatgcc caggttgaac aactgatgac gctggcggag aaatttgacg ttgagtacga
     1741 cggttggggc acttactttg aagatcccaa cggcgaagac ggcgacgatg aagattttgt
     1801 cgatgaagac gatgacgggg ttcgccacta attaattgat taaaatgccc gatagcacac
     1861 actagcgggc atttttttag agttcgcgta gcatccgcac ttcacaatca acatggcccg
     1921 tgcagccaag tgcatagtcg atatgttgaa aacctaaatg ttcatacaaa ccaatcgctt
     1981 ctttcaaaaa agcggtcgtt tccagatagc agcgtttgaa gcccatttct cttgcctcct
     2041 ccatcgcctt taaggccagt ttttttgcca gccctttgcc gcgaatagcc ggaagaaaat
     2101 acatcttttg cagttcgcaa atgtccgatt cactacctgc taatggcgca atcccaccgc
     2161 cgccgaccac ttcaccctcg tactcaacca cccaatatgc atgacccggc tgactataca
     2221 cttgatacag ctcgtcaaga ttcggatcgg cgacggtgta gcctttatca gcggtaagac
     2281 cgtattcggc ggatacctga cgaatgacgc gggcgatagc aggattatcc tgcagcgtca
     2341 ggcgacgcat tacaggtgat tgcggcgcaa tgttattcat agtttaaccc aataaaaata
     2401 aagtaattat acatttgttt aataccactc tcttcttacg tgcgcaagcg tcttataaag
     2461 caataaagtt tttgccagcc tattactgat aagagtattg taacgtggca gaacatttta
     2521 ctgtctgtat ctataaatta tatcaatgga ttttttaatt cagggaattt ttatggctca
     2581 agttattaat gaaatggatg ttccgtccca ttcgtttgtt tttcatggta caggtgagag
     2641 atattttctt atttgtgtgg tgaatgtgtt gttaacgatt ataacgctag gtatctattt
     2701 accatgggca ttaatgaaat gtaagcgtta tctctatgct aatatggaag ttaacggaca
     2761 acgattttct tatggaatta ctggtgggaa tgtttttttt agttgtcttg tttttgtttt
     2821 tttctatttc gcaatcttaa tgacagtgtc agcagatatg ccgcttattg gctgtgtttt
     2881 gactttgtca ctgttggttt tgcttatatt tatggcagca aaaggactgc gttatcaggc
     2941 cttgatgacc agtctcaacg gcgtaagatt tagttttaat tgctctatga aaggggtctg
     3001 gtgggtaacc tttttcttgc cgattttaat ggccattggg atggggactg ttttctttat
     3061 ctcgacaaag atgctacatg ccaatagttc aagtagtgtt attgtatctg tggttctgat
     3121 ggcaatagtt ggtattgttt ccattggtat ttttaatggt actttatata gcctggtaat
     3181 gagttttctc tggagcaata ccagtttcgg tatacatcgt ttcaaggtga aattagatac
     3241 tgcgtattgt ataaaatatg ccatcctcgc atttttagct ttattacctt ttctcgctgt
     3301 tgctggttat attatcttcg atcaaatatt aaatgcgtat gatagttctg tgtatgcaaa
     3361 tgatgatatt gagaatttac agcaatttat ggaaatgcaa cgtaaaatga taatcgcgca
     3421 gttaatctat tattttggga ttgctgttag caccagttat ttaacggtgt cgttgcgaaa
     3481 tcattttatg agcaacctgt cactgaatga tgggcgtatt cgttttcgct caactttaac
     3541 ataccacggt atgctttatc gcatgtgtgc gttggtggtg atatccggga ttacgggcgg
     3601 tctggcttat ccactgctga aaatatggat gattgactgg caggcaaaaa atacgtattt
     3661 gctgggcgat ttggatgacc ttcctttaat caataaagaa gaacaaccag ataaaggctt
     3721 cttagccagt atttcacggg gaattatgcc ttctttacca tttctgtaag agataaaaag
     3781 gccggagcat gctccggcct tcgttttcat cactgtgttt tgattacagc gcggcgataa
     3841 cagcctgctg ttcaatcagc ttcgctttcg cttccgcata gccttccagc ttctcacgct
     3901 ctttcgcgat gaccgcttcc ggtgcgcggg cgacaaagcc ttcattcgcc agcttgttct
     3961 cgatacggct gatttcgcct tcgatcttcg ccacttcttt cgccagacgc gccagctcat
     4021 cttctttgtt gatgaggcca gccatcggga tcagcagctc tgcgccgtcg atgatcttcg
     4081 taacggaaac cggacctttg tcatcggcag gcagcacggt gatgctttcc agacgcgcca
     4141 gggtttgcag gaagccacgg ttttcattta cgcgacgttc tgcatccgcg ctgcaaccac
     4201 gcagcagcag ctccagcggt ttgcccggcg cgatgttcat ttctgcacgg atgttacgta
     4261 ccgcaacgat cgcctgtttc agccattcgg tgtcggccag tgcggcttca tcaacctgag
     4321 atgcatcgta ctgcgggaac ggctgcagca tgatggtgtc ggcagtgata ccgcaaagta
     4381 ctttcacacg ctgccagatg gtttcggtga tgaacggaat gatcggatgc gcgaggcgca
     4441 gcagaccttc cagtacagtc accagcgtat ggcgagtacc gcgcagttct gcttcggtgc
     4501 caccgttcat tactggcttg gtcagctcga gataccagtc acagaactgg ttccaggtaa
     4561 actcatacag aatgcctgcg gcgatatcga agcggaagct gtccagcgct tcgcggtacg
     4621 ctttgatggt ctggttgaac tccgccagaa tccagcggtc cgccagcgac agcgtcattt
     4681 cgccgccgtt gaagccgcaa tcctgacctt ccgtgttcat cagcacaaag cggctggcat
     4741 tccacagctt gttacagaag ttacggtagc cttccagacg cttcatatcc cagttgatgt
     4801 cacgaccggt agacgccagt gccgccaggg tgaagcgcag ggcatcggtg ccgtgcggct
     4861 caataccgtt cgggaactgc ttctcggtgc gcttacggat tttgtccgcc agctgcggct
     4921 gcatcatatt gccggtacgt ttttccagca gttctggcag cgaaataccg tcaaccatat
     4981 ccagcgggtc gataacgtta cccttggatt tggacatctt ctggccttcg tcgtcacgaa
     5041 tcagaccggt catgtaaacg gtgtggaacg gcacctgcgg tttgccattt tcatctttga
     5101 tgaagtgcat ggtcatcatg atcatgcggg caatccagaa gaagatgatg tcgaaaccag
     5161 ataccatcac gctggttggg tggaactggc gcagtgcgtc ggtgttttcc ggccagccta
     5221 gggtagagaa ggtccacagc gcagaagaga accaggtgtc gagaacgtct tcgtcctgac
     5281 gcagggcaac gtcggcaccg aggttatttt ccttgcgcac ttcttcttcg ttgcggccaa
     5341 cataaacgtt acccgcttcg tcgtaccatg ccgggatacg gtgaccccac cacagctgac
     5401 gagagataca ccagtcctga atatcgcgca tccaggagaa gtacatgttt tcgtactgct
     5461 tcggtacgaa ctgaatgtcg ccgttctcaa ccgcttcaac tgccggcttc gccagtacat
     5521 cggcacgtac gtaccactgg tcggtcagca ttggttcgat aactacgccg ccacggtcgc
     5581 cgtaaggaac tgtcaggtcg tgcggtttaa tttcttccag caggccgagt gcgtcaaccg
     5641 cagcaacgac cgctttacgt gcagcaaaac gctcgagttt ctggaactct gccgggattt
     5701 cgctggaata aacgtcagat tcgttacctt tggtatcgaa cacctgggcg ctttcacgga
     5761 tatcgccgtc aaaggtcagg atgttgatca tcggcagcgc gtgacgttta cccacttcat
     5821 agtcgttaaa gtcgtgcgcc ggagtgattt tcacgcagcc ggtgcctttt tccatgtcgg
     5881 cgtgttcgtc gccaacgatc ggaatacgac ggttaaccag cggcagaatg acatatttgc
     5941 caatcagatc tttgtaacgc ggatcttccg ggttaacggc tacgccagta tcgcccagca
     6001 gggtttccgg acgggtagtc gcgaccacca gataatcttt accgtctgcg gttttcgcac
     6061 cgtcagccag cggatagcgg atgtgccaca tcgaaccttt cgattcgcgg ttttccactt
     6121 ccaggtcaga gatagcggtg cgcagtttcg ggtcccagtt taccaggcgt ttgccacggt
     6181 aaatcaggtc ttctttatat agacgaacga aaacttcttt caccgcattg gacaggcctt
     6241 cgtccatcgt gaagcgttca cgttcccagt cgacggagtt gccgagacgg cgcatctgac
     6301 gggtaatcgt gccgccggat tccgctttcc attcccagat tttgtcgatg aaagcttcgc
     6361 ggccgtagtc gtgacgggtt ttaccttctt ctgcggcaat cttgcgctca acgaccatct
     6421 gggtagcgat cccggcgtgg tcagtaccga cctgccacag ggtgtttttg ccctgcatgc
     6481 gctgatagcg gatcatggta tccatgatgg tttgctggaa ggcgtgaccc atatgcaaac
     6541 tgccggtgac gttcggcggc gggatcatga tgcagaaact ttcctggctt tcatcgccat
     6601 taggcttaaa gtagccctgc ttttcccagt gctcgtaaag cggctgttcg atatcttgtg
     6661 ggttatatgt cttttccatt atttccaggt tgccgtattc aggttgaaac cagccacgcg
     6721 gtaggcttta tagcgttcgc gcgccagttg tttcagagaa tcttcataag gaacgaagtc
     6781 taccacttct gtgaaagcgg tggcaaaatc tgcaaagctt gttcgcagac taatcaatat
     6841 atcgcgcggg ctgctgctgc gtttttgcgg ccaggcgatc tccaccggcg caccgccgcg
     6901 cggtccttct cccgctaaat tatgcggaac aaagctttct gccggacgcg cccacagggc
     6961 ttcatccagc cggtaagcct gcttttcatc ttcacaggcg atgagcacgc gcttaccgct
     7021 gcgccaacgt tctgcggcaa tttcacacac cagttgctca acggcgctta agccatcgac
     7081 ggtggtgtca ttgtccagaa ggtagaacgt cgcgtttttc atatatgggg cttcttgtgg
     7141 tggatttaaa tacgcatcag gtatacaccg cagcgcctga tgcgacgctg ttgtgtctta
     7201 tcaggcctac gagttcagtg ctgtgtgtag gtcggataag gcgctcacgc cgcatccgac
     7261 aataacagca ttgcctgatg cgacgctgcc gcgtcttatc aggcctacga gttcggtgct
     7321 gtgtaggtcg gataaggcgt tcatgccgca tccgacaata acagcattgc ctgatgcgac
     7381 gcttgcgcgt cttatcaggc ctacgagttc agtgctgtgt aggtcggata aggcgttcac
     7441 gccgcatccg gcaataacag ccttgcctga cgcaattact cttcgccgtt aaacccagcg
     7501 cggttcaaca ggaactgtgc cagcaacgct accggacgac cggttgcgcc ttttgcttta
     7561 ccagaacgcc aggcggttcc tgcaatatcc aggtgcgccc agttgtactt acgggtaaag
     7621 cgtgacagga agcaacctgc ggtaatcgcc ccaccaggac gaccgccaat gttcgccata
     7681 tcggcaaaat tggactccag ttgttcctga tactcgtcac ccagcggtaa gcgccatgcg
     7741 cggtcaccgg attgttcaga cgcggcaatc agttcatggg ccagcggatt atggttcgcc
     7801 atcagaccgg taatgtgatg acccagcgcg atcacgcagg caccggtcag cgtcgccacg
     7861 tcaatcaccg cttccggctc aaaacgctca acgtaagtta acacgtcgca cagtaccagg
     7921 cggccttcag cgtcggtatt cagcacttca acggtttgac cggacatggt ggttaacaca
     7981 tcgcccggac gataggctcg tccgccaggc atgttttcgc agcctgccaa cacgccgata
     8041 acgttaatcg gcagttgtag ctccgcgacc atccgcatca cgccgtaaac cgctgccgca
     8101 ccgcacatat cgtacttcat ctcatccatg ccttctgaag gcttgatcga gataccgccg
     8161 gagtcgaagg ttaaaccttt acccaccagc acgattgggc gtgcatcttc cgacgcgttg
     8221 cctttgtact caatcaccga catcagcgat tcgttttgcg aaccctgacc gaccgccaga
     8281 taggaatgca tccccagctc tttcatctgc tgttcgccga taacgcgggt gataacattc
     8341 ttgctgtagc tgtcagccag ctggcgcgct tgtgaagcga ggtaagcggc gttacagata
     8401 ttcggcggca tattaccgag atctttcgcg gctttaatcc cggcggcaat cgccagaccg
     8461 tgctggatcg cgcgctcacc gctggtcagt tcacggcggg tcggcacgtt gaacaccatc
     8521 ttacgcagcg gacgacgcgg ttcgctcttg ttcgttttca gctgatcgaa actgtagagc
     8581 gtctcttttg ccgtctcgac agcctgacgc actttccagt agttgttacg gcctttaacg
     8641 tgcagttcag tcagaaagca gaccgcttcc attgagccag tatcattcag cgtattaatg
     8701 gttttctgaa taacctgctt gtactgacgc tcatccagct cacgttcttt gccgcaacca
     8761 ataaggagaa ttcgctcgga aagtacattc ggaacatggt gcagcaacaa tgtctgcccc
     8821 ggttttcctt ccagttcgcc ccgacgtagc agggcgctga tgtacccatc gctgatttta
     8881 tcgagctgtt ctgcaatcgg agaaaggcga cgtggttcga agacgcccac gacgatgcag
     8941 gcactccgct gtttctccgg gctaccgctt tttacactaa actccatgca ctacgctcct
     9001 gaatcttaaa gacaacggcg gtggctacag atagaattgc aagctttcgt aactcatgtc
     9061 cgctgttgcg atgacttcgt gttaatctta acgttattac ggcattggca cgtcagaaca
     9121 aaatctgaga ggtgaatccg ttgagtataa ttatcttagc gacgatttcg acgactcaag
     9181 agaataaatg acgtttaagc catgaaacaa gctaaaatcc tgcaaaagac gagtttttac
     9241 gggcgtattt aaagtgataa tcataagata tctggtgcgg gagacgctca aaagccagct
     9301 ggcgatactc ttcatcttgc ttttgatctt cttctgtcaa aagttagtga ggatcctcgg
     9361 cgcagcggtt gacggcgata ttccggcgaa tctggtgctc tcccttctcg ggttgggcgt
     9421 gccggaaatg gcgcagctta tcctgccatt aagcctgttc ctcgggctgc tgatgacgct
     9481 gggcaaactg tataccgaaa gtgaaattac ggtaatgcat gcctgcggcc tgagcaaagc
     9541 ggttctggtg aaagcggcaa tgatccttgc ggtattcacg gcaatcgtcg cggcggttaa
     9601 cgtgatgtgg gcgggaccgt ggtcatcgcg tcatcaggat gaagtgttag cagaagcgaa
     9661 agcgaaccct ggcatggcgg cgctggcgca agggcaattc cagcaagcga ctaatggcag
     9721 ttcggtgctg ttcatcgaaa gcgttgacgg cagcgatttc aaagatgtgt tcctcgcgca
     9781 aattcgacca aaaggtaatg cacgtccttc tgtggtggtg gccgattccg gacatttaac
     9841 ccagctgcgc gacggctccc aggtcgtcac tctcaaccag ggaacgcgct tcgaaggcac
     9901 tgcactgtta cgtgatttcc gcattacgga tttccaggat tatcaggcga tcattggtca
     9961 ccaggcggtg gcgctcgacc cgaacgatac cgaccagatg gacatgcgca cattgtggaa
    10021 cactgacacc gatcgtgctc gcgcagaact gaactggcgt atcacgttgg tattcaccgt
    10081 gtttatgatg gcacttatgg tcgtaccgct gagcgtggtt aacccacgtc agggacgcgt
    10141 actgtcgatg ctgccagcca tgctgctgta tctacttttc ttcctgatcc agacctccct
    10201 gaaatcgaac ggcggtaaag gtaagctgga cccgacgctg tggatgtgga ccgttaacct
    10261 gatttatctg gctttggcga ttgttctcaa cctttgggac accgtgccgg tccgccgcct
    10321 gcgcgccagt ttttcgcgta aaggagcggt gtgatgcaac cttttggcgt acttgaccgc
    10381 tatatcggta aaactatttt caccaccatc atgatgacgc tgttcatgct ggtgtcgctg
    10441 tcgggcatta tcaagtttgt cgatcagttg aaaaaagccg ggcaggggag ttacgacgcg
    10501 ttaggcgcag gaatgtatac cttgctgagc gtgccgaaag atgtgcaaat attcttcccg
    10561 atggcggctc tgcttggggc gttgcttggt cttgggatgc tggcgcagcg cagcgaactg
    10621 gtggtgatgc aggcttctgg ttttacccgt atgcaggtgg cgctgtcggt gatgaaaacc
    10681 gccattccgc tggtcttgct gacgatggcg attggcgaat gggtcgcgcc gcagggcgag
    10741 cagatggcgc gtaactaccg tgcgcaggcg atgtacggcg gctcgttgct ctctacccag
    10801 caaggcttat gggcgaaaga tggcaacaac ttcgtctaca ttgagcgggt taaaggtgac
    10861 gaagagttag gtggcatcag catttatgcc tttaacgaga atcgtcgtct gcaatccgta
    10921 cgctatgccg ctactgcgaa gtttgacccg gaacataaag tctggcgttt gtcgcaggtt
    10981 gatgaatctg atctgaccaa tccgaaacag attaccggtt cgcagacggt gagcggcacc
    11041 tggaaaacca acctcacgcc ggacaaactg ggcgtggtgg cgctggaccc ggatgcactc
    11101 tctatcagcg gtttgcacaa ctatgtgaag tatctgaagt cgagcggtca ggatgccgga
    11161 cgttatcagc tcaacatgtg gagcaaaatc ttccagccgc tatccgtggc ggtgatgatg
    11221 ctgatggcgc tgtcgttcat ctttggccca ctgcgtagcg taccgatggg cgtgcgtgtg
    11281 gtcaccggta tcagtttcgg ttttgtcttc tacgtactgg accagatctt cggtccgctg
    11341 acgttggttt atggcatccc gccgatcatc ggcgcactgt tgccaagcgc cagcttcttc
    11401 ttaatcagcc tgtggctgtt aatgagaaaa tcgtaaccga atgaacgaat aaagggagcg
    11461 aaagctccct ttattattgt tagcaaagtg tgcttcgttc attcctgaaa aataattaaa
    11521 attcaatata ttgcagtggg gtgggcatga taagacgcgc cagcgtcgca tcaggcaaag
    11581 acaaatctcg cctgacttac cttcttctcc cccccagcaa actccccaac atcccgcgta
    11641 caatctgatt cgtcacttgg cgagcggcgc ttttggccat tgtttgcacc acaccatctt
    11701 tcttcccgcc gcgtggtccg gtagtgccga acaaaatatc cttcaatcca ccaagaatgc
    11761 cgtcatccac cgctacctct ttccctttcg cggggggatt attttgctgc tcggtactgg
    11821 cctgaaagcc tttttgcaac atctcatagg cggattcccg gtccacctca tcctcatatt
    11881 tgccatacac cggagagtga ttaatcaagc cattacgctc atcttccgtc accggcccca
    11941 tccgcgaaca aggcgcgatc accatcgcac gctccaccac agaagggctt cctttcgcat
    12001 ccagaaaaga gatcaacgcc tcgccggtgc ccagttcctg aatcgccttt tcggtatcaa
    12061 acgccggatt ggcccgcatg gtttgcgccg cagcttttac tgctttctga tctttgggcg
    12121 taaaagcccg caaagcgtgc tgaacgcgat taccgagctg gccgagcaca ttatccggaa
    12181 tatcagacgg gttttgcgaa acgaaccaga cgcccacgcc ttttgagcga ataagcctta
    12241 tcacctgctc aatcttatcc agcagtacct gcggtgcatc gttaaacagc agatgtgctt
    12301 cgtcgaagaa aaacaccagt tttggtttct ccagatcgcc cgcttccggc aattgttcat
    12361 acaactctga gagcatccac agcaggctgg cggcgtacag tttcggcatc tgataaagtt
    12421 tctcggcgct gaggatattg ataacgcctt taccgttggc atcggtgcgc atccagtctt
    12481 tgatatccag catcggctca ccaaagaagt gtgctgcgcc ttgctgttcc agcgacaaca
    12541 gcccgcgctg gatggcacca accgatgcgc tactgatatt tccgtactga ttctggaaag
    12601 atttggcgtt atcgccgatg tactgggtaa tcgcccgcag atctttaaag tcgagcagta
    12661 acagcccctg atcgtcagca atgcggaaga tgatattcag cacgccagat tgcacatcgt
    12721 tgagattcaa cagccgcacc agcaacagtg gccccaggtc tgaaaccgtc gcccgtaccg
    12781 aatggccttt ctcgccaaag atatcccaca ccaccaccgg attagcatgc ggttgccagt
    12841 cattgacgtc gatattttta agccttgcga gcagtttttc cgacgccgtt cctgcctgcg
    12901 cgataccggt cagatcgcct ttcacatcag ccataaacac cggcacgccg atttccgaca
    12961 atgactccgc cagtttttgc agcgtgacag ttttacccgt ccccgttgcg ccagtaatca
    13021 gcccgtgacg gttagccatt cccggcagta aaaatagttc tgtgtccggc gtgcgggcaa
    13081 ttaacagggg ttcgctcatg agatatcctc cagtttcctg cctggagtat aggcaacggc
    13141 tgcgcactct tcaccgctaa attcagcaga ttgcgagacg ccttccaaag agatttaagt
    13201 gccgggcgac aggcacccgg caaagagatc agaagtcagc tttcagaacc acgcggtaac
    13261 gcgccttacc gtcgcgcaca tgcttgatgg catcgttaat tttcgacatc gggaacaatt
    13321 cggtagtcgg cgcaaccttg ctgcgggcgg caaagcgcat cagcttacgc aactcataag
    13381 gtgtaccggt agcagagcca gagacactgc gatcgcccgc aatcagcgta aaggccggaa
    13441 ccggcaacgg cgtgagaacc gcaccgaccg tatggaaatt accgccatag gtcagcgcct
    13501 caaaataagg ctgccagtcg aggctgacgt tcacagtatt gataatgaga tcaaactgcc
    13561 ccgacagggc tttcagtgcc tgcggatcgc ggctattcac cactttatcg gcacccatcg
    13621 ccagtacttc ctgctctttc gccggattag aactaaaggc cgtcacctcg catcccattg
    13681 cgtgcagaag ttttatagcg atatgcccca gcccgccaat accaattacc ccaacgcggc
    13741 tggtagcagt gatatggtgc atcaacagtg gtttaaagac cgtgataccg ccgcacaaca
    13801 gcggcccggc ggactcgata tcaatatttt ctggcagtgg aatcacccat tgccagtccg
    13861 cacgcaactt ctcggcaaag ccaccgcgat tcataatcgt cggcacagca ccttgctcgc
    13921 agttgatctg attaccgcta atacaggcgt cgcagtgacc acagctacgc gccgtccagc
    13981 caatcccgac acgctggccg acctgcaaac ctttatcctg cgcggcgctc ccgagcgcca
    14041 ccacgcgccc aatcacctca tgcccggcaa ccagcggata ttgtgaaaat ccccattcat
    14101 tgtcgatcat cgatagatcg gaatggcaga tcccgcaata atccacctgc acttcaacat
    14161 cttgtggcct cagctcaccg ggatcgtact cataaacttc cagttcgccg cccgcttctt
    14221 ttgcggcata gctttttatc atcgacattt tttggtcctt ctctggtgtt gtttgggaag
    14281 tgtagagcat ggcagggcgg ggtgcctgga gtgtgacaaa ggttacacat cgctgtatgc
    14341 aatgctgaaa atttcagcac ttagcgaggt gcgagcaagc tggcgcttgc atggtggcgt
    14401 gcgacaggta taatccacaa cgttttccgc atacctcttc agtgccgaag tggcgaaatc
    14461 ggtagacgca gttgattcaa aatcaaccgt agaaatacgt gccggttcga gtccggcctt
    14521 cggcaccaag actcatctaa actattgttt taaaacaatt taattcataa tcttgcttga
    14581 aatcgcgtag cccctcttac aaaagggtat acgaggcgat gtctcagcga tataaactct
    14641 atcgtcgaac cagcggcatt tatgttgtcc gcattagcgt tccccaacgt tttcgtcgat
    14701 acgcaggaca gtgtgagatt cacacttcaa caggcactca cgatcttcat gaagcaaagc
    14761 agaagtccgc gctcctgttg gcggtctggt atcagacctt acaagagtat gaacaattgg
    14821 attatcgaac tttaagtgac tgcgccccac tgcttgctgg tgagggtatg atctcgcttt
    14881 ctaactttgc tcagtcaatc gagttgccta tatcgcaatt gattcgagag gtggttaatc
    14941 gtaacctccc ggtattctgg ctggcgactg gtcagttcgg tttctatgtt gatgaattta
    15001 atgcagtaga gcgggaaccg ggtgcaaaac gagaaaaaca gtctgatgat gaaaaggatc
    15061 aacctaaaga agtcatcatt ctcaatagcg cgtttgagct gggtatcgag agcttcgcaa
    15121 atggttatct ccgccccttc aatccccggc atactttaga ttgtctgttg agcgctggag
    15181 tatccgaagg agaggctgca tttcgaacta gtggtgataa ccaaagtgga ggttggttct
    15241 tcgatttacc cggcgtagat ataactgctg atagcctgtt gattagcaaa gttcatgctg
    15301 aaggccttcg acttacatgg ctggttaaga ccacgccacc agcagttagc attcaccctg
    15361 ccgtgcctct tgtcgctcct gttatcgcta atgaatatgt tcaccgcaaa cattacaatg
    15421 aaaacttgtc atggcttcgt gaagagtatt tgaaacatcg acgtaagggc aaggtatcag
    15481 aagcggcgct ccgcgatatt cgctattact tcgatttgat gattgaagtg atgggggata
    15541 ttcagttgga agatttcgac cgtgatttcc tccgggctta tgagagcaag ttgcgcacaa
    15601 ttcctgctaa ccgtaatttg atgaaaggta agcacggggt taagacgctg gatgagttaa
    15661 tcgccaaagc ggcagaatgt ggcgataaac tgatgacaga agagtctgtc aaaaagtata
    15721 tcaacggcct ttatggtgca atggagtggg ctgttgatga tggtaagttt ctgaaatcgc
    15781 catgcgacaa ctttttccct cccgatgaca aaggtgagcg agagcaggat cacactgaca
    15841 tatttgaacc gcatgaaatt aaggcaattt tttcgcaacc gtggtttgtc gctggaactg
    15901 ttgaacgtaa tgcgcaaggg cgattccatc aatattgccc gtttcactat tgggcgccgt
    15961 tgttgggctt gatgacgggg gcaagggtta acgagattgc acagttaatg ctggacgatg
    16021 ttctggcaga tgacggcgtt tattacctga accttgaaag cgatagcgaa aacggaaaga
    16081 aactaaaaaa cgccaattcc cgccgcaaga ttccggttca ttctacgctg attgaactcg
    16141 gttttatcga gtatgtggat gcgttgaaag ctgccgggta tgaccgtctt tttcccgagc
    16201 ttaaaccaca taaaaccaaa ggctatggta ggccggtttc cgcatggttc aatgaatcat
    16261 tgcttgcggg tcgattaaaa cttgaaagag acagaagcaa atctttccac tctttccggc
    16321 attctgtttc aactttgctt aaagagaagg gtgttagttc ggaactgcgt gggcagctac
    16381 ttgggcatgt gcgaggcaaa acagaaactg aagtgcgata cagcaaagat ttaaaaccgg
    16441 ttcacatggt tgaggttgtc gaaaagattg atttttcttt gcccgagata gcgagattca
    16501 acattcctga tgggctggat gctgtagaat tgatctgacg gttttctctg acagcataca
    16561 atcaagtcta acagtccgcc ttgagcgagg agcggacact catgattctt acttatatat
    16621 attttcctac atcgaatttt tttaggggaa aattatttag ttagtcacac tccgccagtc
    16681 aattctgcga ttagtggttc ataaggtgcg ccagcttgta gtgcattcca gatttctaaa
    16741 attttatcac gatttctccc caaccaactc gaaacatcac tatcatattt cttaggtaaa
    16801 tctccctcta tgcggtctcc tgattgtatg gcataactgg cagcatgcgc ttgttttcca
    16861 tagtcaatat gaatatgggg aagttgatgt cctttttctt gatatatttt tatcttcatg
    16921 ttctgtctct tcaccaagag aatttccatg aatccgccgc cactagagcg tcttttctca
    16981 gtcagtagat ccttctgagc aaagagtttt tgtaattcgt aaaattcttc atctaaattc
    17041 atatatcacc cattaaattt cagttagtag ttattaaact caactcttta tcgctaggct
    17101 tttcctagtc cacttttggc acaaagctgt ttaggtcaaa tctaagctaa tacacttgat
    17161 tacttacgat tgcatccgca gccctttcac gctttctgat ggtaggctaa tcactgtttt
    17221 aatgcagttc tctatgctgc caatgatgtc ttcgcgtggg ccgctgcgcc agtctgcgct
    17281 tgcgtaaaaa gcatcgagaa ccgcagtcat gcttttagcg ctgtcaaagg cgcgaatcaa
    17341 gtagtagcaa tccaggtcat caagcgagtt gccaaacgaa acaacatcga tcccgtggct
    17401 ttggtgtagg ggaacactga tttcctgcat tatggcgtga aaagcggcac cgctgccttt
    17461 gcgtaaggtg tattgcagta tttcgacggt tctcaagcgt tatctccgtg ttgcccttgg
    17521 gcggctccta gttgatagca ataaagtaaa ccggactggg ccatgaagct actgctaatc
    17581 agacagtcat ccgtaagcag aagctgcgat atgcggttca aacgcgctgt aaggctctct
    17641 ggcgagtgtt ttaaagaagg tagtcgaaat catggtgcag aattcgcggc gctctcatag
    17701 agtgactggt gaagccagtt gaaacaagcg tagcggagtt cgagcaatca atgagaaatc
    17761 tgggcgagct ttagttttac cgcgtcgctt aactttggag gctgattgcc tctataagga
    17821 ctgtctcacc gccacggaaa ttatcggatg caatgcttaa gaggggcgct tccaaaccta
    17881 ggacatccac tttttcgctc ccaacttatc cggctgcatt tacaccgcga acacgttgaa
    17941 cgttagcagc ttctggttcg tgtttggttg ttacgcatgt gaggtaaaaa cggcagcttt
    18001 acttttgacg ggaacgccat aagacaccaa acagcagaga agtgtaattg ttaaaaatgt
    18061 taaaacataa cttgttcttt aagatgtaaa cccttcctcg ggggggaagc cggacatatc
    18121 aaccctaagg ccatctgaga agatagcctg taggtttgct cggttcccct tgccagatga
    18181 agatcaagtc ttcctcgtgg tgtgacgacc tttcttgaaa gatgcattat gcgcaaaaga
    18241 agctacttaa gaatccgccg atagctactt tcaccctctg cttggctgca tcaaacgctt
    18301 gcgcgcccta cctaccacaa tcaacgttac gcagagttcc catcctgacg tcttccgcta
    18361 aacagcggtg atctccacct acccggaccg gtttaccgca tgaggtaggt gtcaggagag
    18421 aagctagcac accatcattt taaagaaaag tcatatatat catatggctg cgttcatatt
    18481 ctgaacgcag ttatcgatgt taacctaaag gtatgagaac actgtcgttt ttcatataca
    18541 aagggtgctt cggctcacct gtagcgttta agacgaagca cattgtgctc tctggcaata
    18601 gctgtcggac ggtcgttgag cgtttgaggt gtcttccttc atttccccat gcggcgacaa
    18661 ccagtccggc accgctaaca agacgtatca agtggctatc gttatctttg ccaatgggat
    18721 cagacgcttt catcatttca tgtcgctggg tgtgaacgaa tgcgaacagg ttggccatgt
    18781 atacgccacc gtagccccag cttttggcaa acgatatgca gcggctcaag gtgcgatcat
    18841 ccttctcggc atcggcgtat gaagggttta gccctatgaa aagggtatac ggttttgatt
    18901 cgtcccagac tcgtgataac gagtagcgat acttaccgca cggtgaaaaa atcgcgtctt
    18961 taatcatttc ggttgtcctt atgaagtgat ggtttcagcc taacatgtct tcacccgttt
    19021 taagcgccaa caaacgaccg ttttccggca tcctccaacc gcacgctttc acactgttgg
    19081 aaaattagcc ttggcttttc cggcaggcca tcctattttc atcaccgttt tctggcaggg
    19141 gccggatgag gagatgacta tggctatttt tggctacggg cgtgtttcga catcccagca
    19201 ggatactgag aaccagcgca tggaacttga acaagctggg tggacgttcg acttttggtt
    19261 ttctgatgta gtcagcggaa aggttcctgc ggtgcaacgc aaagcgtttt ttgaaatgct
    19321 cagcaaaatc cgcgatgggg aaacgttggt agttgcaaag ctggaccgcc tcgggcgtga
    19381 tgcaattgac gttctgcaaa ctgtcaggac gttggctgac cgaaacatca aggttattgt
    19441 ccatcagctt ggcaccactg atctcacctc tgctgccggt aagctcctgt tgtcgatgct
    19501 agcagcggtt gcggagatgg agcgtgacct gctgattgaa cggactaatg cggggctgtt
    19561 acgggcaaaa gctgaaggca ggaaattggg caggcctgct aaaatcgcac cggaagctcg
    19621 tggagcgatt ttagacaaaa gggccgctgg ggttagtgtt agcgcattgg cgcgtgaata
    19681 cggtgtttca agagcgacgc ttgctgcatt actgaataag ggttactgat atacttaaaa
    19741 catgctctaa cactttgcag atacgagcaa tacttggtaa aacagcacac tttccgccag
    19801 aatcggattt ttgaggtcct ggcaacagct aattgctgag aacatttcag taaactgttt
    19861 aactcgctat cagccgaaca ctgatttgta tagaaatgct accctcgttc atactatcaa
    19921 aaataaatta attttgatgc cagccaggtt ggtcattctc aaatacctca gcctcgggga
    19981 atattttagt caatgggata acatatttga ccagatcatg ggtaaaaaag tcatccgcag
    20041 tttcatctac catccacgca gagttctcat ctatgcgtgg cacgagataa tgaacattca
    20101 ggcggactat tttagatttt tgctcaaaat ttactatggc agcatagttt ttttcccttc
    20161 gtttatcggc aacatccttt tgaatatcta catccaaagc tatatgccct acgcctagac
    20221 cataatccaa aagctggtta tctatctctg ctaatgtttt tgttacatgt cgcgaacgtg
    20281 catcgaaaga tttttcattg atacaacgcc atgttatcaa agaagcaagt ttaactttgg
    20341 ttatgaaacg tgagtcgcgc tcatctggtc gtcccatagc aacaacttgg taattttcat
    20401 cttccagagg ggagcctttg ataagacgag caagtttagt gttgactagt aatgagccgt
    20461 tttcagtgat atcttccctg ataagattta agttggccgg tctaatcgtt ccttttccat
    20521 aatcatcatt ccattcataa aatacacctt taaagttttt taagtggcta atgatatagt
    20581 tttcaggcgc gtctttaact tcacataaat atgtgacatc agtccatacg ttcatctttt
    20641 tggatttgat atgaagttcg gcaagatgtg agcgtatatg atgctgtaat atttcctgct
    20701 ttacgtaagg acctttctga agccttttgc attcgacaaa gaaataatcg ccatctccaa
    20761 ggcggcaacg aaactccggg gtttttgcta tccctttttg ttctggaatg aactcaactt
    20821 cataaccttc tgatgcatag tttccagcaa gaaccaactc taataaggct gtgtctggta
    20881 atactgttgt attcttaagc attctgattg cgcgatctct agcgccagta attctatcga
    20941 gcgatttagc acacactcct aattgtttaa cccatggtat tatatttgaa gcattagtaa
    21001 cttcgtaaga ccttctatta tcgataagcg attttgcttg tgcgaaataa ccagcaacca
    21061 cgtcggaacc ataccattct gggtcaaact gtttcccgaa attggcagct ttttgggttg
    21121 tagctatgta aaattgctgt gcttttgcta agcgctggta aaagtgttgt ttgttctctt
    21181 gagttgatgc taaccactca agaccagcaa taacgtcaga atctagcata acaccgtcat
    21241 tcatctttat tccttagttc agccctgaaa ggcactagat caactgagac agattatagt
    21301 tacggaaaga taacgcgatg aatatacgat ggtaagcctc cgctttccac tctatgctgg
    21361 cagctgagtt ctatcatgtt caggcaccta cgtttctaga agttgttcat acagttagta
    21421 taaatatacg tccctgaaat ctttgcgtct tgctttggat gttcatatac gtagtcaaca
    21481 aaagtcttat agagcgttat catctgctgg gtgacgtcgg ccttaccgtt catgttatca
    21541 agtcgctgat aatattctct tttcgtcatc ccactgttac ggccttcggt tacgtttgcg
    21601 gtaaattgag ccatacccat gcacatttga tctttattgt tattcatttg atctttattg
    21661 ttattcgttt tatccgggtg gagagcgcct tccaacactt tatcaccgct gatggcatgt
    21721 tgcgcccacg ctgaattgtt agcgataaac tttttggaga cgatggcaat accgtattca
    21781 acgcgaattc cattgtcgtg ataactgcca tctgcgtctt ttgtcgctag ctttaaagat
    21841 atacggcaac cgggaagcgt tttgtgttct tgcaccgaga taataacggt gtccgtatcg
    21901 caatcagcaa agctgtattc ggggttaacg taagtgttag ttttgattaa cagcccggta
    21961 agaccgtttt tatagccttt tgccggcagc gttttgaagg cgctgcgttc accctttttg
    22021 atcgcttccc ggaagtgatc cataccgatg cgcgtcgcca gttgatcgat gctttctgct
    22081 gcataagatg cagatgacgc agcgattaac ataatggtca gcacagactt ggctgaaaaa
    22141 ccgggcttac taagccgtgt ttttgacatg ttgttatccc tcagaatcca ccccagcggt
    22201 gtcagttatc gcatcataga ccattgattc aaaatgtcac tattggagcg tggctcttta
    22261 gtggacatct gcgcaaattc aggtcatgaa aatgaccctg agaaaactgt ttggacaacg
    22321 agtaaaagag ttgcgcattg ccaccggaat gagccaagaa gcctttgccg atcggtgtgg
    22381 ttttgctcgt agctatatga gccgcattga acgcggtggc tctaatgcgt ctttggatgc
    22441 gattgaggtg ctggctaatg ctctgagtgt tgagccgtgg cgactattgg tgtctgattc
    22501 atctgaagat agtgacccgg aattactggt tccttatgca gctgatggct cgtgttttca
    22561 tcccgggctg gcaagcactc gcgatggctc gtttggcgta ggtgataaaa cggctcagaa
    22621 gcggtttggc acgttttctg aagctcttga gtatcttcgt agcatggaga cagcaaaatg
    22681 gcgtaggcct aatcctagcg gcaactgggg tattgtatct gcggttcggt gggacaagct
    22741 gagaaagtag ctcaagtgta agtttatgaa ttttcatgct gtatggatga acagtgttca
    22801 ttagatgctg atagcctcac gtttcatcag ctctttgttt ctataagagt gaataaaaca
    22861 gcaaaatgcg ggtgcgttac gtaggcgtta cggtggcgtt ttactttgac gctgaatggc
    22921 ttaattcata taaataacat tgagttaaat agttattctg aacaaggtct gacggggttt
    22981 aactggtttc aaaatccgaa cgcagtggcg tttacttaac atccacatag ctgacaaatg
    23041 ttctttcctc ccgacccact agacctccat aaacatcaaa aggcaggtat ggcacgttaa
    23101 tgatttgtgc cgccacgaac actcggtttg attccgtcca gcggatcaaa cttaagatgt
    23161 agcccctaga acaaaaaggt cctacgattg ttcttttaaa ggctaatagg aacgtgatta
    23221 cagcaggtta tggagactta ggttcgagtc cggccttcgg caccaaaagt atgtaaatag
    23281 acctcaactg aggtcttttt ttatgcctga aatccgcgcc acgcaaggct tttccagctt
    23341 ttctcctcaa ctgaagtcaa cctgaaccaa cccacatcaa caggcttgtg agtatactaa
    23401 tgagtatatt tgccgattcg atattgcttg tatactcacg agataccaat ggaaggaaga
    23461 ccattatggc cctaacggat actaaagttc gctctgcgaa acctgaggag aaagagtatt
    23521 cacttgttga cggcgacggc atgtccttac ttgtaaaacc tggcggttca aagtattggc
    23581 gatttcgttt ccgttttggc ggtaaacaac atttgatggc gttcggcgtt tacccggatg
    23641 tttcactggc ggatgcgcgg aagaaaaggg aagaggccag aaagctggta gctgctggta
    23701 tcgatcctcg tgagcataag cgtgctgtga aagaagagca ggcgaaagag attattactt
    23761 tcgagaaggt tgccagagag tggctcgtaa ccaaccaaaa atggtcggaa gatcatgcta
    23821 atcgtgtaaa aaagagcctg gaggacaata tcttcccgac aattggtact cgcaacattg
    23881 ctgaactagg cacccgtgat ctgttaattc ctattaaagc cgtggagaaa tccgggcgtc
    23941 ttgaagtggc ttcccgcctt caacagcgca ccacggccat catgcgttat gcagtgcaaa
    24001 gcgggttaat tgattacgat ccggcgcaag agatgtcggg ggcggttgct tccagtaatc
    24061 gacagcatcg tccggcgctg gagttaaagc gtatccctga gttgcttgac aaaatagaca
    24121 gctacaccgg cagaccgcta acccactgta cgacagaact cactctgctt atctttattc
    24181 ggtccagtaa gctgcatttt gctcgctggt cagaaatcga ttttgaaacg tcaatgtgga
    24241 ccatccctct tgattggtat tgcagtaagc agagggttgg actggtacag taaatatccc
    24301 gcataatcgt gccattcaca tttagagatc ctccggcata atcaatctgc caatgaagga
    24361 gatcgctatt cgtaaagccc cttttactga gcatcagatt atcgccgtga ttaaatcgct
    24421 tgaatacggg cgaactgtta aagatgtggc ttatctgaag ccacttacta caactgcaag
    24481 tccagatacg gcggtatgga attttctgat attaaaataa tcaaggatct tgaggatgag
    24541 aaccaacgtt tcaaatagat gtttgctgat ctcattattt ggtcgggatt tttaaagatg
    24601 tatggaacta aaacgggtct attttcagag ggatttaact gaagcagaaa ttaataaatc
    24661 ataatttata ggtggctggt atttaatatt cctgcatcat atctctgatg caggagcaga
    24721 gaatggctat aaattattag taaacagaat gtctatctct cacccacaaa cccctcataa
    24781 gggtaatttt tacgcacctg cggcaacgca atagttttcc cgtcaaaatc ccagaataat
    24841 ctctcaacga tccctccgcc gtttaccgca tccgagacca gtacccgcat attttcatcc
    24901 aggccaatcg atcctttatc aaaggcttta tggtgtattg cgcaaagcgc cagaccattg
    24961 gggatttcac aagggccgcc gtgctgtttc cattttatat gcgccgctac cagcgcgacg
    25021 gtggtgtcat cgtggcgcat attaaagcca catattgcgc actggtagtt ataagcgcgt
    25081 aatacatttt tgcgaaataa cgggtcgcgc tgtttacggg tttgctggag atcaaaaccc
    25141 agctcgtcag cgatttcttc ctgaatactc tcggtgaagt gcgcttcgag tatctgttgc
    25201 gccagagtat tgatgagttt tttattgccg gttaccaggg cgtagtgctg ttcatcgaaa
    25261 ccgcctgcaa cgtggtattc gttcagttcc ttcactggcg gttgtcggct gctccctgct
    25321 gttgagcaac gctcggcatt gtgtaattgc cagattccgt cgccttgtaa tcgccagaac
    25381 ggcatatcag gtcggtactg tgaacgctgt ggcccaaaac gttccagtaa actgtgcaga
    25441 ggttcgtaga tttccgtgcc ataatcgaaa aggcgcggat gtccattcag gtatcccgct
    25501 aatacgtata gcagtaacaa tggtttatgc ggcgcgcgct gttcaccctt gtgccagatt
    25561 tttatgttgg caattgcctg ctgtagcgat ttactggaag ccattgatat cccaggcagt
    25621 gaaataattg caatcatgct cgcaaatttt ctgctgttca agcctgcaag tgaagatcca
    25681 ttgctgtgtt gagggatcaa aagacaacaa aaacttattg aagaaataat tttctgcttt
    25741 tttgtcgaaa taatgggcat tgatcttgtc tatagctgct gaatcttttc cagcctcgtt
    25801 gagatcttca aaatagccat tgaagatgtt ctggttaaca ccgccagtaa cataagcctt
    25861 gccgttgtgt acaaaagtca cattacccgc catgcccatc ggcgcgtgcg acatcaattt
    25921 attgctttat tcattgtgac tgtcttctgt ctaccaagct ttaaccttca cccatatgag
    25981 ggaagtttta agtatgaggc gaaccatagc atatgctttt atacctgtta caccataaaa
    26041 atcagacaag ttatgccata aactactttt gtaatttgag ccacaaatca gggaaatttt
    26101 atttatcttc cgtgaaagtc ttaactttca cggagtttta gaatttacaa ttttgctggc
    26161 gtaaaggctc ctgtttgtat ttctttaacc agctcaccca cggtatatat tctccatcct
    26221 tcgacttcgg cgggagtaag gttaactgca attttaaggc ctggccatgc agcttctacg
    26281 gttgcaataa tttcatgttc tgagtttgga agatctaacc caggaacagg aggttgaaag
    26341 cctttatgtt gcatcatatt aatatcacca tgcactgatg gatcagtcag gtcatatatt
    26401 tcattccagg attctgcttt taataaccct tgttgggaac gtaaacgttg aatgatagcc
    26461 atctctgctt gggtaacgaa tataccttcg tggaggggct gttttcgatg gcaatccttg
    26521 cacagaacaa gaaggttttc atggtgatta tcatatttga tgccattttt atgatggaca
    26581 tggcacaagt ttttggcggt tgataaattc acgccacaat cattacaaac ataatttgcc
    26641 ttttctcgta tttctttaga tatttccttc cagttttcga catacccact atttgttttg
    26701 tcatagatac ccttaggcat atgacgaaaa caagtactat aatctgaaaa aaactcattt
    26761 aatgagaatg atttaaaaat ctctcttttt cgttgtttat caatacttcc tttataatta
    26821 agtcgttcga ggcaattcat acatacccgt aaatcaacat cctggctccg acctgaacca
    26881 tcatcaattt cgaataaacc atctatgcgg ttagttgcat gatacctttc aaatcgattt
    26941 ttatggcgca tttcatcaag agttctgcac caggcaatat gaaaacgttt tcctttttcg
    27001 ccatctacga tagccgcatc ataacgccct gaatggtccc gaatatataa aagaacctgg
    27061 cggccatgat aagagataag cccgctacca gggtcaagat cttcgatact aacttcttta
    27121 cccgtggata actcaatatc tataggatca aagtcaatag gggattcatc cagaaaaaaa
    27181 tcgactcctt ctggtaacat tttcttccct aattgaataa gttctgaaat gtcgatagat
    27241 aatttcatct cttactcctg aataatcttt ctaatctgca actcggcatt tgttacaact
    27301 ttgaaggtga cacgacgaga gcgattagga tcctccttgc cggttttatc aagaatggga
    27361 tgtgcagatg aataacctac tgcggcaaat ttacttttaa cccattgttg gtgtgtcgcg
    27421 atatttttta tgtcataaac gtattgtaat actgcacgtg tacgaccttg cgatagtgcc
    27481 atattattaa aataagcaat atcaggattc gttgttcctg tccagtcagt acttgtgtga
    27541 ccttcaatgc ggacttcagt aatatgttcc tgataattat ctagaacttt taggtagcga
    27601 ggaaagaagt cgtcaagaat gagtttaaac tttggtttta gctctgtgct tcctaagcca
    27661 aataaaacat ccggtgattt aaatcgaacc tccagagtct gtttatcgat ctctgcatcc
    27721 cagtcttgta aatcctttgc aaactcaata tccagagcat tataaagctg taactgagca
    27781 ttctcgtagg ctacggcaac ttctttaatt ttttcttttt caatacgtac gtagtgcata
    27841 taagcaatag atatgaacat aaaaaccatc atcagcccag ccattaggtc cgacatggat
    27901 acccaatgtt cattatcttg atctttttta ggtaattgtt tccctataat cttatccatg
    27961 atcgattacc cgcgatgttt attagcagaa attaatgctt gtaactgccc cataactatt
    28021 tggtaatctt taataagttt ttcatagtta ccgacaaaac ctttgctgat tgaaagcaga
    28081 gcattaccta atgattgcat ttcacggttc atttcacgtt cagttgcttg ctcaaaagca
    28141 tgtaactgct cgttgatgga tagagcaaat ttttcaattg attctctgac ttgagaagtc
    28201 agccctgcac ttgccttatc aaatgatgtc tggattgaat caaacatatt atcggcatta
    28261 ctggatagtt gatggttaat cttttcacca gtcttactga acatatcaat cacattcgtc
    28321 gtattttgtt ccatatttct atgcaattct ttcgagtgcg actctagtga tttcaggaat
    28381 tcttcaccac tttgtttcat ttcagtaatt gtttcaccta acgtgctggt tagggtttcg
    28441 gaggaattgc tgacatcatg tgccatcgag gcaaatgctt gttgtgtttg ggtaaccgat
    28501 tgtctgaatc cttcggtacc ttcatccagg gcaacgcgca ttgaatctgc attaagaagt
    28561 atttgctggc tggtttgctc aagagatgca ctaacatttg cagctccgga tttcagcagt
    28621 tcacccactt cagccatttt gttctgtatt tcaggtaata cggttgtagc tttatcgcgg
    28681 atggcgacaa aggtttctaa atggcgggag agttcgctga tttgatgttg gttcacctga
    28741 agcacttcac gcagttcaga catagccaga ggaatttctt tacattcttc ccaaatccct
    28801 gcaaccgcag tttttgtttc aaccagggac tcgacacttt gttgatattg ttctgacatc
    28861 tgctcaattt gcgttttata atttccctgc cactcaacaa gtttttttac agaggcatca
    28921 agagctttaa agttttcacc aaactgttca gttaaatttt gattaaaatc aataatgact
    28981 tgtcgcaaag catcaataat ttgttctgta gcaccttttg ccattagatc tgcaaattgt
    29041 tcaagttgtt cccaaagctt attactgaaa tgagtgtgat tagctaattg tgcctgcgag
    29101 gaatcgctaa tctcagtacg tagtaatttt atttgagcaa taagagaacc ttcactatca
    29161 ccgttaatac ccgagactaa tttagtcaga gtctggttct gctcttttaa ttcatgatag
    29221 atatgttcag gtgtaacaga ttcagggtta ttttctgcta acgcacttcg tttattggca
    29281 aaaaagaaag catccattcc attaaataaa atggcaaaaa acatacctac aatgcttgta
    29341 atgaatgctg tttttaggcc acctaataat acggggatgc tggtatcaat actttcggta
    29401 ttaaaattga gtaaaccaat aataatacca atgaaagtac ccagaatacc taatgccccc
    29461 attaaggttg gagcatattc tctaaactta ctatgtatat tttttttgtc acaccaccac
    29521 gctgataaaa atacgaatat aacagcccag atgaaaatat tggtgatcag tgtagagtct
    29581 atcgattgaa acaactgctc aaaaagctgc gctaacattt tgatacctgt gtaaataatg
    29641 gatagttgta atcgtttaaa aaataaacaa aacacacata atttatatgg ttaagttcgg
    29701 tgtagcgctc atgattgtaa caagtttttt cgagatataa atgatttgtc tcatttttag
    29761 cgagttcatc gggttgagct tttttatgat gatagtaacc tgttacgaat tgtatcaaag
    29821 gaaggcaggt tgcttgtgaa aactccagtt aacccacttt tgcaatggct taacatgttt
    29881 tttagtcgcc gttcattatc cggagctgat ggacgggcgt tatatgctta ccgttgtact
    29941 gatacagagt acgaaagttt ggcagaacta ctgcgtacat atgccccccg tagttatcca
    30001 agaacgatat tcatttccta tagcgatgtt ctatttagca tatatgccgc agagtttatc
    30061 cgccggactc atacggtcgg acaccctaaa tgggacacaa ttttagattc tattaactgg
    30121 aaagttccgt atgttcatcg acagaagctg gttaatgatg gcattcgcta ctggaaaaga
    30181 aagataagaa acctgggaca agcttcgggt tatctgcata cactggcttg tgaaggtggt
    30241 ttgcctatcc gcatgattga aaatgagagc ggttatttga ttacctattt cagaaggata
    30301 tatcaggcgc ttcgtgggca atcatctcaa tatcctgccg ctaaaattgc acaagaatta
    30361 ggcgatacga ttcctgtcac aatgcaaaac gaactggtct atgaaatagc cggtgaattc
    30421 tgcgagactc tctgccgatt acttagtgag catcctcctc atagcagtga tcccgtttct
    30481 gcattacgta agctctctcc agactggcat cttcaacttc cactcgttct ccccgaagcg
    30541 aatgctgcag agattgtaag gcgattactt tctcaatctt ctgaaatacg cagtgcaagt
    30601 agtcttcagg ttgaaagaat ctgggtcgat gttgatgaca gttggtattg cgatgcccgg
    30661 ttccgattcc cggccacaat gcgtacagaa cagttaacct ctttgtttga atgccatatt
    30721 cagccggagc agacccgact catcatttca ggaaaatgga aaaatggcgg ggcaaggttg
    30781 gcaatgctaa gccgttatga gcagcaagat tggcgggtcg agttattacc tattgcgatg
    30841 caaaaactct ctggtgcaga tgcaatggcc gaaatctcat tgtcgcttca tgaaggtcca
    30901 attctgttag gtcacacaat tccaaaaggc ggttatgaac ttactgaaga acttccctgg
    30961 gtttttgaag cgatgaatga gagtgaatcg cagctaaaac ttgttggtat ggggtctgtg
    31021 agttccaggc taaatgctct gtttatttcg ctaccgaaaa atagccattt agatattagt
    31081 ggagaaggtg agtttgatat cccaaggttg ctgaaaaaca gtgaacgcag cttaactaaa
    31141 ataagtggag tatttagcgt tgttttacat gatggtgctg tttgtacaat tcgcacccag
    31201 caactttatg attctgcgat tgaatattat attaaatcga cagaagttga actggttaaa
    31261 tcggattatc ctgtccatcg agcctggccg aagatcggtt ggaaaaagga tctgcaatat
    31321 ggcattgtac cggagaaaga acttttttgg cgttccattc gttcaggtaa taatgcctgg
    31381 tattctgtcg catcagaaat gccaaaagga cagatagaag tccggcgaat agttaatgat
    31441 gaggttttat ttagcggtaa agttgtagtt ttaccagcag acttcgatat taatattata
    31501 cctgagagtg ctcagcaagg cattattatg ctttctggta taacggatac tagaattgat
    31561 aaatattcaa acaatgaaaa agtaacactt aagtccgatt attcacaaaa cgagtgtgct
    31621 atttattata attcatcgct gatgctggaa aataccgttg atttacgggt ctcctggaaa
    31681 gatggttcta atcttaaact attattacct aagccggtta gcggtggccg atttgtaact
    31741 aatgatggtt ctgttcattt tgatggtgtg gcatctatag cacatttgca tggaatagat
    31801 gctgagttat taaccatatc atgtgctgga agaggatatc ttaatatcga gttattggat
    31861 gaaaatccag tagctgaaaa attccgctat ttacatgccg accttcctct tttatcagga
    31921 cgcaatgaca aattacaaca aatttcactt tatgagaact ataatttgct aaatgctatg
    31981 ctggcatgtg catggaacag caatagtaca ttatgtgttg atttttactc tgacagattt
    32041 ggaaaggata aagcaacact caatattaaa cgctacgatg gtagttttat tgaacacgat
    32101 caagggttac tggttgatat aaaaaattct gttgtttttc ctgcaaatag aatagacgaa
    32161 ctggttgttg atgctatttc tcttaagaat cctggcttac acatatcatt gttaaaaaaa
    32221 gatgagtttg cttatgatct ttcagctctg aatgttcagg atagtccatg gttaattgtg
    32281 ggaaaacttg atggtacagc tcgcattgca ccagtaatta aatggatgct acctgtattg
    32341 cagacaaatg atttattact aaatgctcta tgcgaagcag acccagaaca gcgcaaaaaa
    32401 aattttaatg agctaatttt tgaaatagat aacaacccat tgcaaaatta ttgctgttta
    32461 ttaacagagt atattaagaa atacaaaatg aataatggct tatccttgct ggatctggac
    32521 ttgttcagag gtatttcgag taattaccgc gtggttgttc aattgttaat atcatcatgt
    32581 ctttctggtg atagcgatac gatttatgat atacaggaag aattaccctt ttcatgggga
    32641 tggattcccg tttcaatctg gaaagatgtt ttccaaaaat gttggactta tctggagaaa
    32701 cagattaacg ataaaacatt agcattacat atattgcaac cctttattgc ttttatgaac
    32761 catcgtgcac atatcgatcg tcggctggct ccgattgcga atatgttact tacatatagt
    32821 gagagcctac caaccggttg tgatgtattg ccaactgtta gtcgtgagca gtttaatgaa
    32881 gctaaacaga tgctattaag gaaccccgac agctttgggc gtatcagtat cttccctaaa
    32941 gaactttggt ctagtgctat tactccagag ttaaaatctg tttttaataa gctttggatt
    33001 gaagataaat atcactcacg gcttgaaaaa cgttttaatt tgatgttagt cgcagcgctg
    33061 ttaacccaaa aagataataa cctgatacat caactgtctg cgctttttga atttcactat
    33121 cagcaagccc cacagcaatt aggggtaatc tatcaatatt attttgaaca agcaggagta
    33181 tgtcattgat ggacgttcag caactgcatt atgcgccact tctaaatcag cttcatcagc
    33241 gtgcggtgag gtcggtggta agtcagttgg cattgcggag caagtcgttg actcattatc
    33301 tggaatcaca atattttcag cctcccggtc aggaggggtc actgcttgca gacccggtat
    33361 tcgaaagtac attcggctgg atgcctgcgg atgaaacaat ggaggatttg cgggacaagc
    33421 tgatctccag cccgctggtt gatgcattga gcaaagcgcc agtgccattt cgtcatcaac
    33481 ttactgcgtg gcgaacgatt ctggaagaca aaaaatcctt gttagtgagc agcggtacgg
    33541 gatccgggaa aacagaatgc tttatggtac cggttctgga agatcttatt cgtcagcaaa
    33601 aatcgacccg ccagtcacta acagggacac aagctatttt cttgtatccg ttaaatgcat
    33661 taattaacag ccaacaggag cggcttcgcg aatggacgcg cggatttaag gggaaaatac
    33721 gcttcgcgct atataatggc gaaacccggc ataccaaata cgaaattcag gaagatcagt
    33781 tgaaagtacc tgaacaagct ttgagtcggg aagccattta tgaagaacca ccttccatca
    33841 tggtgacgaa taccaccatg ctggaatata tgctgattcg tcgtaaagat gccccaatca
    33901 tcgaaaagtc tcaggggatg ttaaagtata tcgtcctgga tgaagcccat agttatattg
    33961 gttctcaggc tgctgagtta gcgctgcttt tacgtcgtgt tatgcaggct ttcaatgttg
    34021 gacccggcac agataaacct gtgcagatta tcgcgacctc tgccactatt ggtgaagact
    34081 ctcctgaagg taataaggag cttgctaaat ttgtcgctga tctcgcgggc gttacagaac
    34141 gtgatgtcaa agtggttcgc ggttatcgac aaataccgcg ggtatcggag tcgctgattc
    34201 gtcatgaata ccccacatct ttaacttctc ttaaatccct gagccctcag gatctttacc
    34261 aacagctttg tcattaccgt gtggcccagc aattgcgtca ggcgttaact catcccggac
    34321 ggcaggcagt acgtttgagt gagttgctca atgttgccag aagaacctgg ccagatatta
    34381 atcaccgtga gctactgcaa ctcctcgatc taatggcaag atcgcgagaa ggtgaactgg
    34441 cttttacgcc attacgtatg catgggttta tcagaacact ggcgggtcta tgggcatgtt
    34501 caaacaaaca atgctcacat aaagcacatg aattaaatca gagtgactgg ccatttggtc
    34561 aggtatggtt tgagcagcgc caatattgtg actgtggagc gccggtattt gaagttttac
    34621 gttgctccgg gtgtggttca gcttatttgt ctgccaaaga ggagatgcgc ggtgatggaa
    34681 cgaactggtt ggtagcgcaa ccagccactg ccgaagtgga tgagtttgct cttgatgtag
    34741 atgtttattc tgaagatgaa gacaacgacg aattagacaa taactctcaa tttgatcgtc
    34801 tgattgcgag cgatggtgaa aaatatatca tttcactgga agaaacgacg gcggggaaaa
    34861 ttgatagtga agcgcaaaag cactatgaaa taaacctgtt aagaccggaa tctcgaggtg
    34921 agggacgcaa taactccttt gcttgtgttt gctgtggtga cactcaacgg aaaaataatc
    34981 ctttattccg tccgcttcgg ctcggcgcgc cctttttttt aaatgaaatt attcctactc
    35041 tgctggagtt ttctccatta ccgcaaacaa gagaagagca gttaggaccg tttaatggtc
    35101 gacgcttgct cacctttacg gattcgcgtc agggtaccgc tcgcatttca gcccgcttac
    35161 agcaggatgc cgataaagcg acgttgcgag ctctttgtta ccaggagctg gcaaatatta
    35221 cagagccttc agcaaaactc agcccggcac actgtgcact gttagagaaa gttattagcc
    35281 agttttctgt cctcccttta aaaaattcca tcgacgcgct gaaaaaagcg atggctgcga
    35341 atgaggagct tagcccgacg gacattcagg cacttaaaat tattgagccg atggtgagtt
    35401 ccttaagcac ggagcatgct gaagcgatgc aaatacttca ggctaactct ggcaatcagc
    35461 agaagaaaac catgtcatgg caaacgctgt ctgaacagct ggttggcagt gtggaccttg
    35521 ccgatagaat gagaggcagt tttaaagaat tgtctgggct tgatcttact aaacaacagt
    35581 ttgcagattt ttgtttatac agcgagtttg gtcgccgccc taaaaatgcc tggaccctcg
    35641 aaagtctggg tctggtagcc attcactatc cattcattga taaagttaca acctgcccgc
    35701 aggactggaa gacattatta cccgatcccg ataagcaatt agcggaatgg cgtaatttat
    35761 tgaagattac tcttgatttc tttatccgtg aaaacagtgc cgttttttac gaaaacgagc
    35821 aataccctcg ctggatgggg gcacgtttcc cggtaaaaat gctccagggg ccagataaaa
    35881 aaaacaaagg gcagaaacgt gatcaacttt ggccacaaat acgcgatcgc cattataacc
    35941 gggtcattaa gctattaatc gcagtctttc cggcaataca acctgagcag gctcactgga
    36001 aatcgttagt taaccactta atgattgagg tctgggatgc aattcgaccc tgcttacgtc
    36061 agtttgagtc gggttatcag ctggatatta aacaacaagc tgaattttac agtccaaagc
    36121 aagtgtggcg ttgcccctat actcgacgtg cgttagacgt cacgttgctg ggatactctc
    36181 cgtatttgcc gggtagcaaa gaaattgcac ctgaaaaagc agtgttgatt gaaatgcctg
    36241 agcttcctgt acgtcactgg cgtttgtctg gtggtggaga aatcgcccga gaagaacgtc
    36301 tggagtggct ggaaagtaat gcgctgattc aacatgcccg cgaggaagga ttatggtcta
    36361 ctcgcagcga ccgactggcc ctgaaagata gctggtatcg tctggaggaa cactctgcgc
    36421 agagaacgcc tgagcagaac cagtttaatg aaaaacaatt taagtcaggc aaagttaatg
    36481 tgctgaactg ttccacgacc atggagatgg gggtggatat tggtggtatg agtttggtgg
    36541 caatgaacaa cgttcctcca gcgccggcaa attatttgca acgtgcaggt cgcgcggggc
    36601 gtcgtggaga gagtgcatct gctgccatta cgttatgtaa aaacacggcg catggaatgg
    36661 aggtattcaa agaccccctc tgggcgttca acactactgc cagtgcgcca cgggttcgtt
    36721 ttgggagcag tagtattgtt caacgacatg ttaatgccct ggttttaggt ctgtttttac
    36781 gagctgaagt tcctgatgcg acaaaactga gttgcaaatg gttctttgaa ggtgatgaga
    36841 gccagtgtct tcgttttctc cattggttaa atcaccaggc cgatcagctg gcggataaac
    36901 ttaaacgcct tactcaggga accgtactga tgtcattgac ggcaacccag ctcttgaccc
    36961 gcacacaagc aatgatgcag caggttgata tccgctggag aagccaactt gcgatactgt
    37021 tggaaaatat cgaagcgttg aaggcggata acagcgcgtg ggaagaaaca ccagcaggta
    37081 aagccatcgc ttatcagctt cgtgattatc gcggtgcata tctgttttct aaactcatta
    37141 gtgaagcgtt cctgccgggg catggtttcc cggttggcgt agtcaatttc aattatctga
    37201 cggcagatga gctggaaaaa cgtcgcgcaa taaaagccac tcaggctgac cctaatgaag
    37261 ggggagaaag cttttctcgt cgtattgaaa aattgcccag ccgtgatttg cctacagcat
    37321 tacgcgagta tgcgcctggt gcagatgtgg tattgggcgg aaaagtatat cgttcctcgg
    37381 gcattatgct tggcaaggtg cttgccagcg ggcaggagct gtcaggcgat catcatattc
    37441 cgtggttctg gcattgccgt aaatgtggtg cgggggcaac cagtactacc catcctgtcg
    37501 aatgtagtca ctgcaaagcg gatatacagc aacttgatgt gaagcgttat ttacagcctg
    37561 ttggttttgc gacagatatt cgttatcagg cacataatga tgtatccatg cctgcacaac
    37621 ttccctggaa agatccacgt gttcttgtac cgtcttcggt gtgggtttct ttacccgatg
    37681 ctgggctagg acgttaccgt tttagccata gtggggaatt gttccacttc agtgaagggg
    37741 aatttgggca cggctacgca atttgcctct catgtggtcg ggcagaatct caaactcaac
    37801 cccagcgtac ccctgaaaat ctaaagaacc cagagaggga aaatacgcat tacctgttgc
    37861 gagggggcag taacgatcgt caggggagca ataaattgtg ccacggtcat gtgcataaag
    37921 atctgtggtt gggatatagc agtcgtacag atatggttga gctgcaactc aatgatgata
    37981 atggcctgtt gatccgtgat gaggttgccg cgcgttccct tgcggtcgct ttgcgtgaag
    38041 ggttagcgca taaattgggt atcgaaaata ctgagctggg cgtgacgacg cagcaagcgc
    38101 gcgatatcaa tggctatacc ggatattcca tcttcattta cgacaataat gcgggtggtg
    38161 caggttatgc agtgcaattg atcgaccatt gggctgatgt ctttaattac gccagaaaat
    38221 tgctcgattg cagttgcgat aaattctgtc accactgctt gctcagctac gacagtcagc
    38281 attatgtcaa cagattagat cgtcaccatg cattaaccct gcttaccaat gtacggttgc
    38341 agcgacttaa cctcgcgcca gaatatcagt acttcggcga tggtagccgg gttgaaacta
    38401 accctctgag tttacgtatt gcgcaatgcc tgaacagcga gatttatgac tcctgctcgc
    38461 tggttcttgc ggggccgcag gaacagtggg actttgccca atggccatta tttaaagagt
    38521 tgctccagtt tgcctcctcc ggtgggaatg ttgagttgct ggtggccacg ccgctggcaa
    38581 acttaactga tagctccaga catcagcttt ccgcgttggc ggcgatgccg ggaggtcgct
    38641 tacaagtgaa atccattgcc actgcgcagc tcatgcaagg aaaggggcgc tggcttgcgc
    38701 aggtgactcg cgaagggcaa agccaacagt gggccgcaga cgacagcgct accgttgcac
    38761 ctggtgaatt atggggacaa agtgcgtctt caccagtagt gacacttaaa ggaacgtccg
    38821 gaaaaacctt ctcgggtcag accttaagtg cagaagattt actgcccgcc ttacccaccg
    38881 gggcggtgcg catcaatctc tgtgaacaac tggacgggcc gctggaaggt tttggttcgc
    38941 gtttctggtc gttagtcacg caacagcacg ctggctggaa gcaggcattt acccgccaca
    39001 aagagataac gcatgttgag tacagcgatc gttatcttaa tagcccattc acggcgcgtt
    39061 tgttaggtga aatcttaacc gagctggttg aacagggaat ggcggaacgc gccagcctca
    39121 ctgtttgcgt caaaaaattg gattacaact ctcgccaaca cgacgctctc tataacgcgt
    39181 ggcttaatga ggaagatcgt cagcaggttg tgaccacttt acttgaagaa ggatatctgg
    39241 ggcctgcgtg gccgggagca attagctggt taacggggga taaccagagt acggagcatg
    39301 gacgagaact gacagtcacc ttcagcgatg gcagtcagca ttatgtcctg ctcgacatgg
    39361 ggcttagcta ctggcgctgt attgaagata ccttctttga ttttgccctg cgagtaccgc
    39421 agcaagttga acggctggca aacacgcgag cgcgagctgt tgccccgggt aatgacctta
    39481 ggagttatat tatcgctgga taaataattg ctcccctggg gatatcttcc aggggagttg
    39541 acccagctca ctttttaata atttttgttt gaaaatcatg cgcaatttat gcgcattccc
    39601 tcccattttg tcactttgcc gggggccttc atgatatctt taaggccctc ttttttatcg
    39661 cagtgtgccg cgccacattt ttcaggatga cgttaccgcc actgccccaa atagacagaa
    39721 aaaccgatga ataagcaaaa ttatgcacct ggtatgcggg tggttattcg tgatgccgaa
    39781 tggcgtattc gccgggcgga tgacagtggt gatggtgggt atttgctgac ctgcgatggt
    39841 atttcagagc ttgtgcgcgg taaagaaggg ttatttctga ccaaactgga acaaaaagta
    39901 gagatcctgg atcccgcaaa aacgcatttg gtggaagatg aatccgccaa ttatcaagcg
    39961 gcacagttgt atattgaaag tcagttacgc caacgcgtac cgacagacag taaagtccat
    40021 tttggtcatc tggcggcgat ggattccatg ccgtttcagc tcgatcctac gcggatggcg
    40081 ctggcacaac cgcgtcagcg gatactcatt gccgacgctg ttggtctggg taaaacgctg
    40141 gaagccggta ttctggtttc ggaactgatc cgtcgtgggc gcggaaaacg tattctggtt
    40201 ctggcggtta aatcgatgct gacacaattc cagaaggagt tctggagccg ttttgcgatc
    40261 ccattgaccc gcctggattc tgctggttta caaaaggtgc gtaaccgcat cccaaccaac
    40321 cacaacccgt tccactattt tgacaagacc attatctcca tcgatacgct gaaacaagac
    40381 attgaatatc gtcaccatct ggaaaatgcc tggtgggata ttattgtcat tgatgaggcc
    40441 cataacgtcg cagaacgtgg aaccagttcg ttacgcagta aactggcaaa actgctcgcc
    40501 gggcgcagcg atacgctgat tatgctttca gcaacaccgc atgatggtaa ggctgaaagt
    40561 tttgccagcc tgatgaacat gcttgacccg acggcgatcg ccaacccgaa agagtatgaa
    40621 tacgccgatt ttgccgataa aaatctggtt gtccgtcgct tcaaaaaaga cgtgaaagat
    40681 cagatgtccg gcgagttccc cgagcgcaat attgtcaaat tgactcgcct ggcatccggt
    40741 gccgaagaag aagcataccg tcgtctggtt gagagccagt tccgtgatga tgacgatgaa
    40801 caggcccagt ctaacaaagg tcgtctgttc aagataaccc tcgaaaaagc gctattttcc
    40861 agcccgatgg cgtgtgccag cgtcgtggcg aatcgtctga agcgtcttga gagccgtaaa
    40921 gatcataata gccagagcca gatcaacgaa ctggagtcat tgctgttggc gctgaataac
    40981 attgatgcca gccagtttag caaataccag ttgctgctcg acaccatccg aaaagacctc
    41041 gcctggaaag ccaataacac ggaagatcgc ctggtgatct tcaccgaaag tattaaaacg
    41101 ctggagttcc tcgaacagca actgcgagcg gatctgaaat tgaaagatga ccagatcgcc
    41161 acgctgcgcg gcgatcaggg cgatacggta ttgatggaaa ccgtagaagc ctttggtaaa
    41221 acgcagtcgc cgttgcgtct tctggtttgt tcggatgtgg catctgaagg gatcaacctg
    41281 catcacctta gccacaaaat gattcacttt gatattccgt ggtcgttgat ggtattccag
    41341 caacgtaacg ggcgtattga ccgctatggg caaaaacatc agccccaaat ccgctatctg
    41401 cttaccgagg ccagcgaacc acagatcaat ggcgatatgc gcgtactgga agtgttgatc
    41461 aacaaagacg aacaggcgca gaagaacatt ggggacagtt cggagtttac cggcaaattt
    41521 acccaggaag aggaagaaga gcaggttgca gagttcatga tgcaggatga tggtgccagc
    41581 ctgtttgatc aactgctgaa cagcaacgtc tcggaaagcg ccgaacacga tctgtttggc
    41641 gaaatatgca gtgcggtttc cagcgatgcc tcaatggtca ccgaaacaga caccagcttg
    41701 tttgccagcg aacaggcgta ctgcgaaaga gcattgggtt acctgaaagc cagtggtcaa
    41761 actatccagt atgaaacctt gcctgataat accttgtcgc tggtggcacc ggaggagtta
    41821 cgccgccgct tcaaccagct accgcctgaa attgccccgg agaactggca gctctattta
    41881 agtcaggata aaaccgtcat caccgacgcg attgcgcgcg ctcgcggtga gcaacatgcc
    41941 tggcccgatg tgcagtatct ctggcaaatc aacccggtag tgcagtggct ggacgataaa
    42001 atctcttcgg cttttggtcg tcatcaggct ccggttatcc ggctgccata cctgcttgaa
    42061 cccgatgaag atcacttcat tctttccggg ttattcccga accgtaaatc acatccgatg
    42121 gtgaacccgt ggatagtggt gagctttaac cgtgaatcgc taatcggcag tcagcctttc
    42181 gctgagtttt tacaacgtca tccgcagttg agtaacaagc tgactaacag cggcggtaaa
    42241 gatcgtaatc accaacgcca gcaggattta ctggaagcgg ctattgctca tgcccgggag
    42301 gtattcatcc atgatcggaa tgcgtttgaa acgcacatta accagcaact gaatgagcat
    42361 ctgcaaaagc tggacgtttt gcgtgggcgg cagttgagcc aacttgagct ggattttgcc
    42421 gataacaaac agcaattgtc agtcaagcag agccgtaaag agcagagaca acgcgaaatc
    42481 gaacacaatt ttgacagcta tatcgaatgg attgaggaca ccatgacgac tgaaaaagaa
    42541 ccctacattc aggtaattgc tgttatcacc ggagcggagg gttaatcatg gcgctggtcg
    42601 gtattaataa cgaaaacgaa ttttactcta accactattt gggtgaggtt ttcaccagtg
    42661 atatccgcga tgtgctggaa ccctggatag cccaggaaaa tgcagcgcgt gaagcggagc
    42721 gtgccgctcg tgaacagggc aaagacgtgg agccgggata ccgcgctccg tggaaccagt
    42781 ttaacagtct ggcgactgag tttttccgca aacttgccga gcacgaaaaa cagcgtcaga
    42841 tcccgcagcg tctggccgat caacgtaatc gctggcagcc attgttaaag gcgctgggct
    42901 acgaaattac gccacagatc caaatgctgg atgacgatac accgctgccg gtactggcgc
    42961 gttacaacag cactgacggt agcccgtggc tgtggattgt tgaagcacac gatcaggaag
    43021 aaggaacgct ggatccgctg gcgctctcct tactgaccgc gcaattcccg gcggataccg
    43081 acaaacataa gcacgacagc ctgcgcaaaa aagccaacgg tgaatatcgc agctggcagg
    43141 atttgctctc tacggcggtc ttcacccaaa atgaaccgcc gcgttttgtg ctgctgctcg
    43201 gtaaccgtca gctattgttg ttggaccgta ctaagtgggc gcaaaaccgt ctgctacgtt
    43261 ttgattttga agagatttta agccgtcgtg aaacggatac gctgaaagcg actgcggtgt
    43321 tgttacataa agattctctg ctgccgggca gtggggcacc ttatcttgac tcgctggatg
    43381 acaattcgca caaacatgcg tttggtgttt cggaagatct gaaatatgcc ctgcgcgaaa
    43441 gcattgagtt gctgggcaac gaagcgatgc attatctgat cgaccgtggc ctggcaaact
    43501 atacaggtaa tcgtgcggtg gacccggatg aactgagccg cgaatgtctg cgttacatgt
    43561 accgcctgct gttcctgttc tacattgaag cgcgcccgga gctgggttat gcgccaatga
    43621 ccgccaaaac ctatctgcaa ggttacagcc tggaaacgtt gcgcgatctg gagatgatcc
    43681 cgctgaccag cgaagaagat cgcaacgggc gctacttcca cgacagcctg aatatgctgt
    43741 ttaaactggt gcgcgaaggc tacaacggcg gcgtgaaaat gcagagtgac ctggagagcg
    43801 gcgaccggat caccatccat agtcatcagt tcagcgtccc gcgtctggaa agtcatctgt
    43861 ttgatgccaa caacacgcgc attcttaacc gcgtggtatt ccgtaacgaa accctgcaac
    43921 agattatcca ggcgatgtcg ttaagccgcc cggccaaagg gcgctttaac cgccgcggac
    43981 gtatttctta tcgccagttg ggtatcaacc agttgggtgc ggtgtatgag gcgctgctct
    44041 cctatcgcgg attcttcgcc agcgaagatc tctacgaggt gaagaaagcc ggggaagagt
    44101 ttaacgagct ggagacgggt tacttcgtca gtaaggatga gattagcaaa taccacgaag
    44161 acgagaaggt ctacgagaaa gacggcagtc tgcgcattca ccgcaaaggc agctttatct
    44221 accgtatggc cgggcgcgac cgtgagaaat ctgcctctta ttacaccccg gaagtgctga
    44281 cccgctcact ggttaaatat gccctgaaag aactgtttaa ggagcagatt gatcccatta
    44341 gcgatccgca cgccaaagct gatgccatct taaacctcac cgtgtgcgaa ccggcgatgg
    44401 gcagcgcggc gttccttaac gaagccatca accagctggc ggaagcgtat ctgttccaca
    44461 agcagcaggc ggaaggtcgc cgtattccgc aggatcgtta cacccaggag ttacagcggg
    44521 tgaaaatgta cattgccgac aacaacgttt tcggcgtgga cttaaacccg gtggcggtgg
    44581 aactggcgga agtgtcgctg tggctgaacg ccattagtgg cgatgcgttt gtaccgtggt
    44641 ttggttacca gctgcactgc ggtaactcgc tggtgggcgc gcgccgtcag gtgttcaaca
    44701 agagcgaact gacctacaaa aaagccaaag atccgagctg gcttaacagc gagccggtcg
    44761 aactggcgat gaacacgccg cgtgaagaga cgcagatttt ccacttcctg ctgcccgacg
    44821 gcggtatggc taactacagc gataaaactg ttaagcagcg ttatccggat gacttcaaag
    44881 cgctggacag ctggcgcaaa gagtttatta aaagctttgc cgggcatgag attgctgatg
    44941 tgcagcgtat cagcgaaaag gtggaagcac tgtggaacac ctatcgccag caacttaaag
    45001 cagaacgtct gaaaaccgcc gacagctacc cggtgtggcc ggcagaaaac agcgagcaga
    45061 cgcgttcttc gctgagcagt aaagatgaaa cctttagcgg tcgtcttgaa gataacagcg
    45121 cctaccagaa gctgcgttgg gtaatggact actggtgcgc gctgtggttc tggccgatcg
    45181 acaaagccga tgagctaccg gatcgcggca cctggttgtt tgagattgaa accctgcttg
    45241 acgggattgt aatcacggaa aaagtcactg aagttgcgga gcacaccacc ggcgatctgt
    45301 ttgccgaaga aggcctgctg cgagaagagt cttcgctgtt ttctgttgct ggtcgtctga
    45361 aaaccgaggt gttgttccgt catttgccgc gtctggcgat tgtcgatgcc ctgagaaagc
    45421 agcaccgttt cttccactgg gatctggagt tctgcgacct gtttgccgag cgcggcggtt
    45481 ttgacctgat gctcggaaac ccgccgtggc tgaaagtgga atggcaggaa gcgggcgtgc
    45541 tgggtgatta cgagccggaa tttgtgctgc gtaagctgag cgcctcgaag ctggcaacgt
    45601 tgcgtattga tacctttaac cagatcccgg cgctggaagc ggcctggcgc agcgagtatg
    45661 aaggctgtga agggatgcaa aacttcctga atgcgcagca gaactacccg gtactgcgcg
    45721 gggtgcagac caacttgtat aaatgctttc tgccgcaggc ctggcgatta ggggcgcaga
    45781 aaggcgtggc aggtttcctg cacccggaag ggatttatga tgacccgaaa ggcgggcaat
    45841 tacgtgcggc ggtatatccg aggctgaggg cgcattttca gtttcagaat gagttaaatt
    45901 tgtttgttga agttgatcac catgcgaagt ttagcagcaa tatttattct gctagcccta
    45961 gcacagtggg atttgaacat atatctaatt tgtatgctcc gcaaactatt gatgcatgtt
    46021 ttgaacattc tggcagtggg gacattcccg gtctcaaaga cgagattgag agcgagggaa
    46081 aattaaaagt tgtatggaac acatctggcc accgttctcg attaataagt atcgccactc
    46141 atgagctaga attatttgct cgtctatatg acagcgaagg aacgccagcc tggcaggcac
    46201 gtttgccagc cttacatgct aaacaacttg ttgctgtact ggaaaagttt gctaatcagc
    46261 cgaatagatt aggtgatttg caggggcagt atttttcaac ggttatgttc gatgaaacat
    46321 atgctcagag ggatgggaca attttacggc agactcaatt ccctcaagat tcatcacaat
    46381 gggtactgtc tggccctcat ttctttgttg ggacgccgtt ctacaagact ccgcgcgaaa
    46441 actgtacgct taacagcgat tatgactgcc tggacttgct aactctgcct gacgactatc
    46501 tgccgcgcac taactacatt ccggcatgtg atgcacagga gtatgcaaaa cgtactccat
    46561 gcgttacatg gactgaactg gctgaagatg aaccgaagaa ggtaacagat tattatcgct
    46621 tagctatcag agccatgttg gctcaatcgg gggaacgtac actaattagt gctatttatc
    46681 cgccagaaat aagtcacatg aacgcagtac gttcttactg ctatagctca cagaatctgt
    46741 tactcgaaca ttcaggtatg tgtttttctt taccttttga ttttatttgt aaatctactg
    46801 gcaaggcaaa cttacatcag atgcttgatg gtttctcata cgtattattc aatccgagac
    46861 aaaaggcatt attatactgc ttagtattat cattaaattc tgtaaatgat gtatatgctg
    46921 gcctttggca atcctgctac accccagact tcaacaccca gcgttggagc cgcgatctcc
    46981 cgcagctccc ccaggatttc ttcgccaaac tgaccccaga gtggcagcgt aactgcgctt
    47041 tacgctctga ctacagtcgt cgtcaggcgc tggtggaaat cgacgtattg gtggcgcagg
    47101 cgctggggtt aactctcgaa gagctgctta ccatttatcg cgttcagttc ccggtgatgc
    47161 gccagtacga agcggatacc tggtacgatc aaaacggtcg cattatcttt accccaagca
    47221 aagggctggt gggcgttggc ttgcctcgca ccgcgcgtaa agctgacctg aaaaacggct
    47281 ttgtctttaa cgtcgacagc ccggagtgga ccggcggtga ctgcaccgat caagctatcg
    47341 gttgggatga tgtcaaacat cttaaaaccg gtaccgtcag cgtcaccttt gatgattata
    47401 cccgcagcga cgaaggtgag cgccgtaccg tcacctggca ggctccgttt atcaagccag
    47461 atcgcgaaga tgactacaaa gtggcctggg cgttctttgc acaagataag gagagcgcct
    47521 gatgttgccg tccgttgtca gtcggcaggt cgccgacagc gttgccgcct ttttacgggc
    47581 ggcgttcccg ctaaacagcc cgctgtttaa cggtgagaat aacgataatg tctcaatgct
    47641 ggagcaattt ctggcccagc cggaaacgct gctgaaagga ccataccttt ccgcccagtt
    47701 gccgtttcgt aaaagtgact taccgctgaa ttttttccct aatctgacgc tgccgttccc
    47761 gccgcacgcg catcaggccc gggcatttca gcgtctggga agcgacgcgc cacagccgac
    47821 gctggtcgca accggtaccg gctccggtaa aacagaatgt tttatgttcc cgctgctcaa
    47881 ccactgcgcg ggcgcgtcac aagccggggt taaagcgatt atcatctacc cgatgaacgc
    47941 cctggcaacc gatcaggcca gccgtttcgc caaaaccatc gccagcgatc cacaactgca
    48001 tggcaaggtg accgtcggcc tgtttgtcgg cgacagcgaa atcgaaccca gcaaaaaaat
    48061 gtcggcagaa aaggtcatta cctgtaagca cacgctgcgc gaaagcccgc cggacatcct
    48121 gttgaccaac tacaaaatgc tcgactacct gctgatgcgc cctggcgatc agccgctctg
    48181 gcgttacaac cagcccggtt cattgcgtta cctggtcgtg gatgaattgc atacgtttga
    48241 tggcgcgcaa gggtcggatc tggcctgtct ggtgcgacgt ttgaagcatc acataggtgt
    48301 ggataaccaa cgattcgcct gcgtcgggac atcagctacc gtcggcgacg aactggggca
    48361 actgctcgat tacgcaaaaa caatctttga ccagccgttt accgatgacg ccgttattcg
    48421 cgaagatcgc tacacggcag cggagtactt gcagaattac gccattgcgt acagtcagta
    48481 tccggggccg gaggtcgctt cggccctgga cccgcagagt tacgctaccc cggtagcgta
    48541 tcttaacggg caaatccctt tgtggttccc cgacagcgac ttgcaactcc cggacgatct
    48601 ggagagcgac gacgggcgtg aaaagcgtat tgcgctgggg tcactgttac gccgccacag
    48661 cgtgatgcat gtcttactcc atgatctgca aggcgggatc ctctctgaac gagagtgtct
    48721 ggaaaatctc caggtcatgc tggcagaaag cgccgaccat gcccgacgcg tgctgcaaag
    48781 cattctggca ctgatcgcgc aggcacgtct cgaagtaccg gaaacggaac acgatcggca
    48841 aaaacgtctc caggcgaaga aagcgcgtcc ggtgctgccg tttgtgcaac tgcgctccca
    48901 gttgtggctt cgtgagatgc gtcgtctggt cgccagcgtg gcgaaaacgc cccgactggt
    48961 gtttgccgat gatgtggcgg tggaagatcg cgaacactat ttgccggtta tccactgccg
    49021 tgactgccac gcaactggct gggccagcct gcgtcatggg caaagcgttc agttggaaac
    49081 agacctcgac ggcatttatc gccagttctt tgaaaaaggg cgctccatcg tccttgcttt
    49141 cccggataac aacgaaaaag cggtcagtgg cgcacaccag aaactgtgtc ctacctgtct
    49201 gaaactgaac cgacagtcca gcgtccagtg tggacattgc ggtcatacgg aactgttgca
    49261 ggtactgatc ccggatatgc tcaaagagcg taaggacgag ctggagtttg ctaatgaatg
    49321 cccgtactgc aacagcaaac aggggatttc gattctgggg tcacgggctg ccagtctgtc
    49381 ggcggtgatg attcaccagc tttatggcag ccagttcaac gaacataaaa agctgattac
    49441 gttttctgac tcggtacaag acgccgcgca tcgtgccagc ttctttactg cccgtacctg
    49501 gccgctgatg attcgtgggc aaatcgccag tgttcttagc gaaaaagaga cattaccgct
    49561 ggatgcgttc gcggcgcgtg tttgccaaca aacgcgtgaa cgttcaccgg acgatgctca
    49621 ttttgcagca aacgtcatcg caccaaatat gcagtggctg aatgattatc agacgctgaa
    49681 aaatgaaggc aaactggcgg agaactccga tctgccggag ctggtttccc tgcgcctcga
    49741 ctgggaggtg tacagcgaat ttaccctgcg cgcgcgtatc ggacgtacgc tggaagccac
    49801 cggttatgcc gttgccggag tggataaaga tcgcttcaat ccggcaatta ccgccctgca
    49861 tcaacagctt tgtgaagagc tgggtgatga gatgtctggt gtgagcgtcg agcaggtagc
    49921 gcacctggcg ctgggggtta tctggtatca acgccaggcc ggggccgtta tgcatccggc
    49981 gttcgaaagt tatcgccgtg atggcacgac ctttatgatg acgcaaaaag agcgtacctc
    50041 gcgctatatg ccttctatcg gtccgaaaac gcgtaaaccg gcttatgcgt cgtttgaaaa
    50101 aattaatggt ttccatcgcc tgattggcgc gaagtctaac cccagttggt atcagcattg
    50161 gatcaatcgc acgctgagta acggcaataa tctgtttatc agtagcgttg cggaaacagt
    50221 gttacgacgc ctgtttacgg cgttgaaaat agccggagta gtaaaagatt ttgacaccaa
    50281 agggcgtgaa gcctggggac tggtgccttc ggcgctggtg gtgagccgtg acgtcgttca
    50341 gcttaattgc tcatgttgcc gtgaggttgt ccgggctccg gccgatcaag gctggcagtg
    50401 gcgtaatgcg ccttgtctct ctttacgttg tgaaggtcgt taccaggaag tcgacaatac
    50461 ggcgacctgg cgttgggaga acatggacat cgcgcgcgta cagggcgcag aacacacggg
    50521 gctgcttagc cgtgaagatc gcgaagtcac cgaaaaatcg ttctaccgcg gcaaccaacc
    50581 gtggaatatc aacctgttgt cggcaacacc gacgctggag atgggtattg acgttgggga
    50641 tctttccacc gttttactct gttctgtccc acctgctcag gccaactact tgcagcgtat
    50701 tggtcgcgcc ggtcgtaaag atggtaacgc gttaaacatt accgtggcag aagggaaccc
    50761 gcacgatcag tttttctttg aagaaccact ggagatgatg cagggccagg tgcaggctcc
    50821 tggcgtcttc cttaatgcaa cggctattct cgaacgccag ctggcagctt tttgtatgga
    50881 taactgggtg aaaaccggtg taccagaatc cgctatctgt aaaaacgtca aacagatgct
    50941 ggatgagctg gagttcgggc gtaaatcggg ttttccgtat aacttactgc gatatatcga
    51001 acagcatcat gcggagattg ctgcgcaatt tacggcaatc ttccccgatc ttgccgcaga
    51061 aactcgccaa cagctgttgt cttatcttca gggcgcgcca ggacaacgct cgctggtgca
    51121 gcgtatcgaa gaagcgttaa aactgctggt ggaagatcgc aaatctttgc gctcacgcat
    51181 cgataaactc aagcgcagta tcgataaact ggagaacgcg ccgcgcgatc aaaactttga
    51241 tagcgatatg cgcgagttaa cttcggaacg gcaggcgctg atggcgctgg tcaatcaaat
    51301 caacaataag caaacgctga atttcctcac ggatgaaggt ctgctgccta actacgcctt
    51361 cccggaagcc ggtattacgt tacgttctgt gctctggcgg cgtaaagaag gcggggaagc
    51421 acgtgaatat caaaacacca ctttcgaata tgaacgcccg gcatcaacgg cgctggctga
    51481 gctggctccg ctgaataatt tctatgccgg tgggcataaa gttgaaattg agcagataga
    51541 cctgaaggtc tctgagccgg agaactggcg tatctgtagc cactgtaact acagcgaaaa
    51601 tatcgaccag acgggcgacc agcataaata ttgcccgaaa tgtggcacac cggggtgggc
    51661 cgatgccgga caaaaaacca cgctgctgaa acttcgccag gtttatgccc gcgccagcgc
    51721 acgtgatagc cagatcagcg acgagagcga cagccgtgaa cctgctttct tccagcgtca
    51781 gctattggtt agtttcgaaa aagaagatgt atcggcggca tacgcgattg aggaaggcga
    51841 agttccgttt ggttttgaat ttttaagcaa agtcaccctg cgggatatca acttcggcaa
    51901 aatggcggat gatgccaatg agttgatgat tgccggagaa gcgaagaagc gaaccgggtt
    51961 taaagtttgc ctcgggtgcg gcatggtgca gcgtcctcgc gatcatgaac cacgtcatga
    52021 tttgagctgc aaatatcgca cggaaccgga aaaggcgaag tttgaagatt atctctacct
    52081 ctatcgccag ctggagtcag aagcgttgcg tattctgctc ccggtaagca gttacagtaa
    52141 tgaccgtgtc gtggaggctt ctcttggggc ggcgatccag ctcggtctta aacattactt
    52201 caaggggaat gtcgatcacc tgaaaggcgt tgtgtaccgc gagccggaaa atgaaggcga
    52261 atcctggcgg cagtatctgg tgatttacga taccgtcccg ggggggactg gttctcttaa
    52321 agaactgatg cgcactcccg acaatttgct gaaattactt gaactggcct atcaggcaat
    52381 agtggaatgc agttgcaaca atgatacgca taaagatggt tgttatcgtt gcgtttacgc
    52441 ttaccgtgac cgtgggcgca tgaagtatgt ctcccgcgat caggcgcgtc tcttgctggc
    52501 gaaaattttg caggcaagag attcgatacg ggtaattgat tccatcaaaa acatttccct
    52561 cgaagccatg atgggcagtg agctggaaaa acggtttatt agctgcctgc aagaaaataa
    52621 aaacctgatt gtcagtcgca gttacgcgca tcagggcgct gggtggatta ttaacacccg
    52681 cagcgaaccg atgatgagct ggcatcttaa agctcaggtg gatttaggcg ctaaagaggc
    52741 ggtggggatc ccttgtcgcc cggattacgt gctatatccg cttatgcagt ctgatgccat
    52801 taaaccggtg gctattttcc ttgatggctt tgcgttccac aaaaatagcg tcgcagagga
    52861 tgtgcaaaaa cgccaggcga ttcgggatag cggtaatttc tgggtgtgga cagtcacgtg
    52921 ggccgatctc aaagagccag ggcttaaaca tgttcaggat gtcctgggac ttgggcataa
    52981 cccggatatg aaacagccta agttttataa cctgtttcat gataccaact ttgcggccct
    53041 tgaagcgtcg ttccgggaac gcaacagttt tgcactgctg ctggattatc tcgccgatcc
    53101 gggcggaaga acgcagttat ggcagagaat ggctgctgca catgcctggg tgtggttgga
    53161 tgtgaagaag tcacaggacg cgggaacgaa acaaaagtat gcctatgaaa tgcaggaaaa
    53221 cgcgccagct tggcggctag cagagctttt gccagacgaa ccgtttgtct ttggtggttt
    53281 actcgatagc tgtaactcat cgcagcaatt tattgagctg gcttcggtgt taccacaaca
    53341 agccattaaa ccgacgacca gcgtcgcaca aatgcgcagc tggctgcgtt tgcatatctg
    53401 ttttgacgat cgctatacgc aggatgacgg ttatgaagcg ggactcaatg gcttctggcg
    53461 actggtaaac ctgttacagt tcctgccgga tatgaccttt accagccgta aagccgtgca
    53521 tttaccgcag gaaagcgttg acactaaagt ttcggcaacg ccagaggcag taccagagac
    53581 tgatgagagt tgggctgaga ttattgagtt tggcttatta agcgctgaag agatcgcgtt
    53641 gcttcagtct cattctctct ctgcgccgac tctcggctac gaattgcaaa atgatgaagg
    53701 tgaaattatt gcggaagccg atctcgcctg gccatcacaa aaatgggcat tgattattga
    53761 taatcaagaa tttattccat tattcacaac caggggttgg cacgttgcat tcgggccaat
    53821 tgacgaaaat acattgcaac atctgtccgg aggtgacaaa tgacggtgca aacgcccaag
    53881 gtggcacttt ctgatggttt tctgggcgca ttcgcgcgga tcccaaaggc acagcaaaaa
    53941 aaggtgcagg aattcatctc caaattccgt caggacccga ccagtaatgg acttaactat
    54001 gaaaagatcc acgatgcgcg cagtaaaaat gtccattcgg tgcgaatcga tcaaacttat
    54061 cgcggcattg ttctgaaacc tgaacagggt gccctgtata tgctgatgtg ggtggataaa
    54121 cacgacgaag catatgactg ggcgcggcgg catgattgtt caattcaccc ggttaccggc
    54181 gcgattcagg tcattgatat tagctatatc aaaccggcag cggaaacagt ggttgataaa
    54241 ccgaaactct tcgctgctta cagcgcggaa cagattctgg ctctgggtgt gccgcctgtc
    54301 tttattgatc aggtgatggc gcttaccgat gaagccgggc ttaatcaact tgaaagcata
    54361 atgccagcag aagcctggga acctctgcac tggctggcag aagggctgga ttaccaggaa
    54421 gtgctggaag agtttaacga tcatcgtgat gagcctgttg ataccaacga ctttatggag
    54481 gcgattgaac gaagtaaacg tcgcttccat gtcgttgaaa atgaacagga gctgttgcag
    54541 atgcttaatg cgcctttaga aaaatggcgt gtatttctgc atccgagcca gcgaaaattg
    54601 gtggagtcgc ccgctaatgg tccggtacga gtactgggcg gtgcaggtac agggaaaacg
    54661 gttgttgcga tgcaccgtgc gcggtggtta tctcagcgtc tggcagataa acccggtaag
    54721 aaagtacttt tcacgacctt tacgcgtaac ctggcggcag atattcgcgc taatctccag
    54781 cggttgtgta cgcgagaaga gatggcgcgt attgacgtcg ttaacattga tgcctggatg
    54841 agcgaccaac taaaacgcca cggttatgat ttccgcgtgg tatacgacag cgatgaaggg
    54901 cgacgcaaat gctggaacta tgccttgcag caggcacctg ccgaactggg gctacctgat
    54961 aatttctatg ctgaagagtg gcaacgcgtt gtccagccaa atgcggttta cacccgggag
    55021 gaatacctca aagtcagccg ggttggacgt ggtacagcgt tatcccgtat tcaacgggcg
    55081 aaaatctggc cagtctttga agaattcaga gcacaaatgg cgcgtgccaa actgcgcgaa
    55141 atgggcgatg ccatgcatga ggctattgtt ctgtttaaag aaaagcaggt ccaactacct
    55201 tatagcagta ttgtggtcga tgaagctcaa gatatcggtg ccccggcatt tactcttatt
    55261 cgcagcctgg ttcccgagtc gcccaacgat ctgtttattg tcggggatgg gcatcagcgt
    55321 atttatcgca ataaggtcgt tcttggtcag tgtggtatta acgtccgtgg tcgtcgcagt
    55381 aaaaaattga agattaacta tcggaccaca gaagagacgc gtcaatttgc tgttgggctg
    55441 ttaactggtg tgaaggttga taacctcgat ggcgaagcgg ataccagtaa tgattatttg
    55501 tcattactgc acggggaaaa gcccatgatt acgcatgcgg cagatttcaa agaagaggct
    55561 gcaacgctcg tcaagcaaat tcaggcattg ctggcgaatc aggttcgttc tcaggatatc
    55621 tgcattactg cgcgtactaa acatgcttgt gaccgctacg catcagcact gaatgacgcc
    55681 ggaattgaga catttaattt aggcaatgat agcggtgaca gcgatgcacg ccctggcgtg
    55741 cgggttgcaa cgatgcaccg tattaaaggg cttgagttcc agtatgtgtt cctggtgggg
    55801 attaacgaag gcgttgtgcc ggaaataaaa gcattagcat ccgatgatcc cgttgaacag
    55861 cgtgatgcat tatttaatga gcgcgcgtta ctgcatgtgg ctgcaacccg tgccgtaaaa
    55921 gggctgtttg tttcatcatc tggtgtccct agtccgctat tagtggctga ctgataatat
    55981 gtccaaaaca ggccaggagc cgaaacgctc ctggtctata taaccgttct ggcaactggc
    56041 gatatttata ttgcttagct cccaacagcc agacaatatc aacttagaaa ttatattcgc
    56101 ttatgcggtt tgcatattgc acagtacaac ctgttttctc tatatctaac gtaacgtcag
    56161 gatgtggatg atttttggat ggggtgacac actggacaaa tatagctggc acgaaaaggc
    56221 tggcatttcc agctgaccaa tagtctctgg aaccatgatg atgataaaat aaatatctta
    56281 agttttaaac gtatgatgcc aggattacca gaattataaa cgaatatatt agttcttttc
    56341 tatttgttca ggaggggtat aaaaattgca attgttaata gtatcgtcag gacgataaaa
    56401 agataatcat gacaaaggca attgaactaa aatgattgac attttacagg tggtttgtca
    56461 gtgatttctt gttattcata atttattttc cgtaaaaaat agtaaggtga tcacctgaat
    56521 aaaggtaatc tcatattttg atgagtatag tgatttttta tttctctggt gaggaatgaa
    56581 ggaaatatca atggtaagga acgtcagaga atcagggaaa attaaggtcc ctcagaaaca
    56641 caatgccgtt cacttaagcg gggcggcact gtgtttcaca ggataacggg tttttgatat
    56701 cttaaccgcc ctcgcccttg atggtcgggg gcgttttgtt atgaacacca cttccagagc
    56761 ccccgcagat gctccagtcg tttcgggata gtccccggtg acgccgtgtt tcccagcact
    56821 atcatttctg tcacgataaa ctggaacgcg aacttaaagc tgatctctga cgccgctttt
    56881 gttgcctcac gccgtatcgc tccgccggta ggcgcggtca tggcattcag tagaatgtga
    56941 ttcccagatt cattaacgcc accagacgca tgacggggta ggcgctctgc cgctcagtgg
    57001 atgtgttggc agagccataa tattcacgca gctcgggttc atcaggtgtc ctgaactgtg
    57061 cgccatcaat ggcaaaaagt tgcaggccgt gccagtcatc cttcaggtaa cgttccgcgc
    57121 cccatgtctg tgtggtctgg cggaagagcc attccaccgg ggcggccccc acgcgctgac
    57181 gcgcctgggt gacagcgctg cgggcgccag caggttcatc cccgcttcgc catccacgct
    57241 caggttaaga cgacgaacgg tcgcatgagc agaaagcgtc aggaagtgct gtatccactc
    57301 ggtgggaagg tgctctgcaa acagttgcgt agagggtggt ggcataagcg gatggtcact
    57361 gaaagtggta taagaaaatg gctccttgtt gtggaagtcg ttatagtgcc gcacgccgga
    57421 ggagttaaaa gcgctgatgg atgcgaataa atcaaattaa tgaacgctca cctttcgcgc
    57481 caaaactgca aaattgcttc tacaaacctg tcagaaataa tcccccgacg ggctgctgag
    57541 ctgaaatgac tgcttcgcag tgccgtcgtc cagttctccg gtgaccggta cgctgaaccg
    57601 taatatcccg tgcttaaatc gtccggatct tcatcaggcg cgttcgtcag ccctcttgca
    57661 ccttgttgct ggaagtcgac gaaaataata ttagctaaaa tattattttg ataattgcca
    57721 taaatagctt catacgcatg agggaaattt tctttccagt accaggtggt atcgccgcaa
    57781 aaccacggcg cgtcagtaat attattaagc tgagaatggt attgttttag atccctacga
    57841 aaggcttcaa ccatatgatt aaagtgttga ggatgtgacg cgtagtcact ggtcattaag
    57901 tcaaattcgc cttgcatcca gcatacgccg aggaatttgt tctgcggatt ttttaccagt
    57961 gcggctcgcg ttctgctgac taaatcctgg tatagcggag tatccgttcc ccaacgacaa
    58021 gcatcatggc tggctccgtg ccgttctgaa tatgtcccct cgctgcctgc ggtaaaagcc
    58081 gatccgccac ggcaacacgg aacgatgaga atccctgcat tatcaggaat gaagggcagt
    58141 aatttccgtg caatatgcag tgcctggcca acggtgccgt actgtgtttg atgattcgtt
    58201 gccagaggat ggtgataact ctgcatatcc tgaacatcgt gtgggcagtg agtcagtgga
    58261 ataatgtcgt taaagtgaca tgacgggcct ccgggatgcg tatgcgcaaa tctcgctaat
    58321 tgtttaattc tgggatgagg cgcatcttcc ctgtccggta atggcagtcc ttcgccatac
    58381 gccatggcat tagactgacc agcaacagta agaacatagt aataatcagg cgatattatt
    58441 gcgttcatat aaatttcgcg taattgaaat gacaaattga tagcagggcc cctttaccgg
    58501 ggccctgaag cgttagtttt gtactgtgac tttattatcc ttcacagaga tcaaaactga
    58561 atccgtcacc gctttgccgc ctgcagtttc accgccaata atcaatagac tattattcca
    58621 gggcaatgat actccgtagg cccgaccttg cgataattca cccgatttat cccatttccc
    58681 gttatgccaa agatgaatat cagtgctata tgattttttc aggccttcat gcgcatagtt
    58741 cttaccgttt tggtaatttt ctcgtgaacc tttgaatccg gcccctccgg caaatataag
    58801 agaatcattg cttatccccg caaaaccgcc agcgacgcca tctggtgatg agacgggagc
    58861 aagcctattc cattttaaat tattaccggt gaaatcaagt tcaaatacgg catccgttcg
    58921 caatccgggt ttggcttcgc cattaataag ccaggtttta tcacctttat tcacaaccgc
    58981 cgcaccagcc gtcccgtacc agggagattc gccag
//
LOCUS       DABSYT020000019        54264 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.019, whole genome shotgun sequence.
ACCESSION   DABSYT020000019 DABSYT020000000
VERSION     DABSYT020000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 54264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.019"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..101)
                     /locus_tag="ID725_RS07675"
                     /old_locus_tag="pgaptmp_001535"
     CDS             complement(<1..101)
                     /locus_tag="ID725_RS07675"
                     /old_locus_tag="pgaptmp_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850165.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLT"
     gene            complement(219..977)
                     /gene="ydjF"
                     /locus_tag="ID725_RS07680"
                     /old_locus_tag="pgaptmp_001536"
     CDS             complement(219..977)
                     /gene="ydjF"
                     /locus_tag="ID725_RS07680"
                     /old_locus_tag="pgaptmp_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGV7850166.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(1114..2094)
                     /gene="ydjG"
                     /locus_tag="ID725_RS07685"
                     /old_locus_tag="pgaptmp_001537"
     CDS             complement(1114..2094)
                     /gene="ydjG"
                     /locus_tag="ID725_RS07685"
                     /old_locus_tag="pgaptmp_001537"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HGV7850167.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            complement(2104..3036)
                     /gene="ydjH"
                     /locus_tag="ID725_RS07690"
                     /old_locus_tag="pgaptmp_001538"
     CDS             complement(2104..3036)
                     /gene="ydjH"
                     /locus_tag="ID725_RS07690"
                     /old_locus_tag="pgaptmp_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGV7850168.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(3041..3877)
                     /gene="ydjI"
                     /locus_tag="ID725_RS07695"
                     /old_locus_tag="pgaptmp_001539"
     CDS             complement(3041..3877)
                     /gene="ydjI"
                     /locus_tag="ID725_RS07695"
                     /old_locus_tag="pgaptmp_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGV7850169.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(3898..4941)
                     /gene="ydjJ"
                     /locus_tag="ID725_RS07700"
                     /old_locus_tag="pgaptmp_001540"
     CDS             complement(3898..4941)
                     /gene="ydjJ"
                     /locus_tag="ID725_RS07700"
                     /old_locus_tag="pgaptmp_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HGV7850170.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(4958..6337)
                     /gene="ydjK"
                     /locus_tag="ID725_RS07705"
                     /old_locus_tag="pgaptmp_001541"
     CDS             complement(4958..6337)
                     /gene="ydjK"
                     /locus_tag="ID725_RS07705"
                     /old_locus_tag="pgaptmp_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850171.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(6364..7440)
                     /gene="ydjL"
                     /locus_tag="ID725_RS07710"
                     /old_locus_tag="pgaptmp_001542"
     CDS             complement(6364..7440)
                     /gene="ydjL"
                     /locus_tag="ID725_RS07710"
                     /old_locus_tag="pgaptmp_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGV7850172.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(7810..8082)
                     /gene="yeaC"
                     /locus_tag="ID725_RS07715"
                     /old_locus_tag="pgaptmp_001543"
     CDS             complement(7810..8082)
                     /gene="yeaC"
                     /locus_tag="ID725_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGV7850173.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8124..8537)
                     /gene="msrB"
                     /locus_tag="ID725_RS07720"
                     /old_locus_tag="pgaptmp_001544"
     CDS             complement(8124..8537)
                     /gene="msrB"
                     /locus_tag="ID725_RS07720"
                     /old_locus_tag="pgaptmp_001544"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGV7850174.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8879..9874
                     /gene="gapA"
                     /locus_tag="ID725_RS07725"
                     /old_locus_tag="pgaptmp_001545"
     CDS             8879..9874
                     /gene="gapA"
                     /locus_tag="ID725_RS07725"
                     /old_locus_tag="pgaptmp_001545"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGV7850175.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9958..10842
                     /gene="yeaD"
                     /locus_tag="ID725_RS07730"
                     /old_locus_tag="pgaptmp_001546"
     CDS             9958..10842
                     /gene="yeaD"
                     /locus_tag="ID725_RS07730"
                     /old_locus_tag="pgaptmp_001546"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGV7850176.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10893..11747)
                     /gene="yeaE"
                     /locus_tag="ID725_RS07735"
                     /old_locus_tag="pgaptmp_001547"
     CDS             complement(10893..11747)
                     /gene="yeaE"
                     /locus_tag="ID725_RS07735"
                     /old_locus_tag="pgaptmp_001547"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGV7850177.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11837..12583)
                     /gene="mipA"
                     /locus_tag="ID725_RS07740"
                     /old_locus_tag="pgaptmp_001548"
     CDS             complement(11837..12583)
                     /gene="mipA"
                     /locus_tag="ID725_RS07740"
                     /old_locus_tag="pgaptmp_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGV7850178.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            13019..14953
                     /gene="yeaG"
                     /locus_tag="ID725_RS07745"
                     /old_locus_tag="pgaptmp_001549"
     CDS             13019..14953
                     /gene="yeaG"
                     /locus_tag="ID725_RS07745"
                     /old_locus_tag="pgaptmp_001549"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGV7850179.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15066..16349
                     /gene="yeaH"
                     /locus_tag="ID725_RS07750"
                     /old_locus_tag="pgaptmp_001550"
     CDS             15066..16349
                     /gene="yeaH"
                     /locus_tag="ID725_RS07750"
                     /old_locus_tag="pgaptmp_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGV7850180.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            16496..17971
                     /gene="cdgI"
                     /locus_tag="ID725_RS07755"
                     /old_locus_tag="pgaptmp_001551"
     CDS             16496..17971
                     /gene="cdgI"
                     /locus_tag="ID725_RS07755"
                     /old_locus_tag="pgaptmp_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGV7850181.1"
                     /translation="MILSTRISLGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIHPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            18152..19642
                     /gene="dgcJ"
                     /locus_tag="ID725_RS07760"
                     /old_locus_tag="pgaptmp_001552"
     CDS             18152..19642
                     /gene="dgcJ"
                     /locus_tag="ID725_RS07760"
                     /old_locus_tag="pgaptmp_001552"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGV7850182.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            19685..20188
                     /gene="yeaK"
                     /locus_tag="ID725_RS07765"
                     /old_locus_tag="pgaptmp_001553"
     CDS             19685..20188
                     /gene="yeaK"
                     /locus_tag="ID725_RS07765"
                     /old_locus_tag="pgaptmp_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGV7850183.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(20189..20293)
                     /gene="yoaI"
                     /locus_tag="ID725_RS07770"
                     /old_locus_tag="pgaptmp_001554"
     CDS             complement(20189..20293)
                     /gene="yoaI"
                     /locus_tag="ID725_RS07770"
                     /old_locus_tag="pgaptmp_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGV7850184.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            20463..20909
                     /gene="yeaL"
                     /locus_tag="ID725_RS07775"
                     /old_locus_tag="pgaptmp_001555"
     CDS             20463..20909
                     /gene="yeaL"
                     /locus_tag="ID725_RS07775"
                     /old_locus_tag="pgaptmp_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGV7850185.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(20866..21687)
                     /gene="nimR"
                     /locus_tag="ID725_RS07780"
                     /old_locus_tag="pgaptmp_001556"
     CDS             complement(20866..21687)
                     /gene="nimR"
                     /locus_tag="ID725_RS07780"
                     /old_locus_tag="pgaptmp_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGV7850186.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            21784..22965
                     /gene="nimT"
                     /locus_tag="ID725_RS07785"
                     /old_locus_tag="pgaptmp_001557"
     CDS             21784..22965
                     /gene="nimT"
                     /locus_tag="ID725_RS07785"
                     /old_locus_tag="pgaptmp_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGV7850187.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            23020..23367
                     /gene="yeaO"
                     /locus_tag="ID725_RS07790"
                     /old_locus_tag="pgaptmp_001558"
     CDS             23020..23367
                     /gene="yeaO"
                     /locus_tag="ID725_RS07790"
                     /old_locus_tag="pgaptmp_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGV7850188.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(23389..23643)
                     /gene="yoaF"
                     /locus_tag="ID725_RS07795"
                     /old_locus_tag="pgaptmp_001559"
     CDS             complement(23389..23643)
                     /gene="yoaF"
                     /locus_tag="ID725_RS07795"
                     /old_locus_tag="pgaptmp_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGV7850189.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            23826..24851
                     /gene="dgcP"
                     /locus_tag="ID725_RS07800"
                     /old_locus_tag="pgaptmp_001560"
     CDS             23826..24851
                     /gene="dgcP"
                     /locus_tag="ID725_RS07800"
                     /old_locus_tag="pgaptmp_001560"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGV7850190.1"
                     /translation="MSDQIIARISQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNQKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            24884..24982
                     /gene="yoaK"
                     /locus_tag="ID725_RS07805"
                     /old_locus_tag="pgaptmp_001561"
     CDS             24884..24982
                     /gene="yoaK"
                     /locus_tag="ID725_RS07805"
                     /old_locus_tag="pgaptmp_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGV7850191.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            24985..25059
                     /gene="yoaJ"
                     /locus_tag="ID725_RS07810"
                     /old_locus_tag="pgaptmp_001562"
     CDS             24985..25059
                     /gene="yoaJ"
                     /locus_tag="ID725_RS07810"
                     /old_locus_tag="pgaptmp_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGV7850192.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(25118..25366)
                     /gene="yeaQ"
                     /locus_tag="ID725_RS07815"
                     /old_locus_tag="pgaptmp_001563"
     CDS             complement(25118..25366)
                     /gene="yeaQ"
                     /locus_tag="ID725_RS07815"
                     /old_locus_tag="pgaptmp_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGV7850193.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(25513..25695)
                     /gene="yoaG"
                     /locus_tag="ID725_RS07820"
                     /old_locus_tag="pgaptmp_001564"
     CDS             complement(25513..25695)
                     /gene="yoaG"
                     /locus_tag="ID725_RS07820"
                     /old_locus_tag="pgaptmp_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGV7850194.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(25699..26058)
                     /gene="yeaR"
                     /locus_tag="ID725_RS07825"
                     /old_locus_tag="pgaptmp_001565"
     CDS             complement(25699..26058)
                     /gene="yeaR"
                     /locus_tag="ID725_RS07825"
                     /old_locus_tag="pgaptmp_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGV7850195.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(26231..26869)
                     /gene="leuE"
                     /locus_tag="ID725_RS07830"
                     /old_locus_tag="pgaptmp_001566"
     CDS             complement(26231..26869)
                     /gene="leuE"
                     /locus_tag="ID725_RS07830"
                     /old_locus_tag="pgaptmp_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGV7850196.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(26996..27919)
                     /gene="dmlR"
                     /locus_tag="ID725_RS07835"
                     /old_locus_tag="pgaptmp_001567"
     CDS             complement(26996..27919)
                     /gene="dmlR"
                     /locus_tag="ID725_RS07835"
                     /old_locus_tag="pgaptmp_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGV7850197.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            28022..29107
                     /gene="dmlA"
                     /locus_tag="ID725_RS07840"
                     /old_locus_tag="pgaptmp_001568"
     CDS             28022..29107
                     /gene="dmlA"
                     /locus_tag="ID725_RS07840"
                     /old_locus_tag="pgaptmp_001568"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGV7850198.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            29358..30968
                     /gene="yeaV"
                     /locus_tag="ID725_RS07845"
                     /old_locus_tag="pgaptmp_001569"
     CDS             29358..30968
                     /gene="yeaV"
                     /locus_tag="ID725_RS07845"
                     /old_locus_tag="pgaptmp_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGV7850199.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            31000..32124
                     /gene="yeaW"
                     /locus_tag="ID725_RS07850"
                     /old_locus_tag="pgaptmp_001570"
     CDS             31000..32124
                     /gene="yeaW"
                     /locus_tag="ID725_RS07850"
                     /old_locus_tag="pgaptmp_001570"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGV7850200.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            32180..33145
                     /gene="yeaX"
                     /locus_tag="ID725_RS07855"
                     /old_locus_tag="pgaptmp_001571"
     CDS             32180..33145
                     /gene="yeaX"
                     /locus_tag="ID725_RS07855"
                     /old_locus_tag="pgaptmp_001571"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGV7850201.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(33199..34314)
                     /gene="rnd"
                     /locus_tag="ID725_RS07860"
                     /old_locus_tag="pgaptmp_001572"
     CDS             complement(33199..34314)
                     /gene="rnd"
                     /locus_tag="ID725_RS07860"
                     /old_locus_tag="pgaptmp_001572"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGV7850202.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(34396..36081)
                     /gene="fadD"
                     /locus_tag="ID725_RS07865"
                     /old_locus_tag="pgaptmp_001573"
     CDS             complement(34396..36081)
                     /gene="fadD"
                     /locus_tag="ID725_RS07865"
                     /old_locus_tag="pgaptmp_001573"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGV7850203.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(36286..36867)
                     /gene="yeaY"
                     /locus_tag="ID725_RS07870"
                     /old_locus_tag="pgaptmp_001574"
     CDS             complement(36286..36867)
                     /gene="yeaY"
                     /locus_tag="ID725_RS07870"
                     /old_locus_tag="pgaptmp_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGV7850204.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(36907..37602)
                     /gene="tsaB"
                     /locus_tag="ID725_RS07875"
                     /old_locus_tag="pgaptmp_001575"
     CDS             complement(36907..37602)
                     /gene="tsaB"
                     /locus_tag="ID725_RS07875"
                     /old_locus_tag="pgaptmp_001575"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGV7850205.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(37660..39570)
                     /gene="yoaA"
                     /locus_tag="ID725_RS07880"
                     /old_locus_tag="pgaptmp_001576"
     CDS             complement(37660..39570)
                     /gene="yoaA"
                     /locus_tag="ID725_RS07880"
                     /old_locus_tag="pgaptmp_001576"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGV7850206.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            39702..40046
                     /gene="yoaB"
                     /locus_tag="ID725_RS07885"
                     /old_locus_tag="pgaptmp_001577"
     CDS             39702..40046
                     /gene="yoaB"
                     /locus_tag="ID725_RS07885"
                     /old_locus_tag="pgaptmp_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGV7850207.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            40468..40767
                     /gene="yoaC"
                     /locus_tag="ID725_RS07890"
                     /old_locus_tag="pgaptmp_001578"
     CDS             40468..40767
                     /gene="yoaC"
                     /locus_tag="ID725_RS07890"
                     /old_locus_tag="pgaptmp_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGV7850208.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(40887..41066)
                     /gene="yoaH"
                     /locus_tag="ID725_RS07895"
                     /old_locus_tag="pgaptmp_001579"
     CDS             complement(40887..41066)
                     /gene="yoaH"
                     /locus_tag="ID725_RS07895"
                     /old_locus_tag="pgaptmp_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGV7850209.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            41140..42501
                     /gene="pabB"
                     /locus_tag="ID725_RS07900"
                     /old_locus_tag="pgaptmp_001580"
     CDS             41140..42501
                     /gene="pabB"
                     /locus_tag="ID725_RS07900"
                     /old_locus_tag="pgaptmp_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGV7850210.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            42505..43083
                     /gene="nudL"
                     /locus_tag="ID725_RS07905"
                     /old_locus_tag="pgaptmp_001581"
     CDS             42505..43083
                     /gene="nudL"
                     /locus_tag="ID725_RS07905"
                     /old_locus_tag="pgaptmp_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGV7850211.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            43267..44631
                     /gene="sdaA"
                     /locus_tag="ID725_RS07910"
                     /old_locus_tag="pgaptmp_001582"
     CDS             43267..44631
                     /gene="sdaA"
                     /locus_tag="ID725_RS07910"
                     /old_locus_tag="pgaptmp_001582"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGV7850212.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            44762..46360
                     /gene="pdeD"
                     /locus_tag="ID725_RS07915"
                     /old_locus_tag="pgaptmp_001583"
     CDS             44762..46360
                     /gene="pdeD"
                     /locus_tag="ID725_RS07915"
                     /old_locus_tag="pgaptmp_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGV7850213.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(46364..47920)
                     /gene="yoaE"
                     /locus_tag="ID725_RS07920"
                     /old_locus_tag="pgaptmp_001584"
     CDS             complement(46364..47920)
                     /gene="yoaE"
                     /locus_tag="ID725_RS07920"
                     /old_locus_tag="pgaptmp_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGV7850214.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(47908..48066)
                     /gene="yoaL"
                     /locus_tag="ID725_RS07925"
                     /old_locus_tag="pgaptmp_001585"
     CDS             complement(47908..48066)
                     /gene="yoaL"
                     /locus_tag="ID725_RS07925"
                     /old_locus_tag="pgaptmp_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGV7850215.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            48383..49354
                     /gene="manX"
                     /locus_tag="ID725_RS07930"
                     /old_locus_tag="pgaptmp_001586"
     CDS             48383..49354
                     /gene="manX"
                     /locus_tag="ID725_RS07930"
                     /old_locus_tag="pgaptmp_001586"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGV7850216.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            49417..50217
                     /gene="manY"
                     /locus_tag="ID725_RS07935"
                     /old_locus_tag="pgaptmp_001587"
     CDS             49417..50217
                     /gene="manY"
                     /locus_tag="ID725_RS07935"
                     /old_locus_tag="pgaptmp_001587"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGV7850217.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            50230..51081
                     /gene="manZ"
                     /locus_tag="ID725_RS07940"
                     /old_locus_tag="pgaptmp_001588"
     CDS             50230..51081
                     /gene="manZ"
                     /locus_tag="ID725_RS07940"
                     /old_locus_tag="pgaptmp_001588"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGV7850218.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            51136..51594
                     /gene="yobD"
                     /locus_tag="ID725_RS07945"
                     /old_locus_tag="pgaptmp_001589"
     CDS             51136..51594
                     /gene="yobD"
                     /locus_tag="ID725_RS07945"
                     /old_locus_tag="pgaptmp_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGV7850219.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
     gene            51905..52027
                     /locus_tag="ID725_RS07950"
                     /old_locus_tag="pgaptmp_001590"
     CDS             51905..52027
                     /locus_tag="ID725_RS07950"
                     /old_locus_tag="pgaptmp_001590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850220.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            52024..52590
                     /gene="mntP"
                     /locus_tag="ID725_RS07955"
                     /old_locus_tag="pgaptmp_001591"
     CDS             52024..52590
                     /gene="mntP"
                     /locus_tag="ID725_RS07955"
                     /old_locus_tag="pgaptmp_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGV7850221.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(52587..53380)
                     /gene="rlmA"
                     /locus_tag="ID725_RS07960"
                     /old_locus_tag="pgaptmp_001592"
                     /pseudo
     CDS             complement(52587..53380)
                     /gene="rlmA"
                     /locus_tag="ID725_RS07960"
                     /old_locus_tag="pgaptmp_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
     gene            complement(53546..53755)
                     /gene="cspE"
                     /locus_tag="ID725_RS07965"
                     /old_locus_tag="pgaptmp_001593"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(53546..53755)
                     /gene="cspE"
                     /locus_tag="ID725_RS07965"
                     /old_locus_tag="pgaptmp_001593"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGV7850222.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(53768..53911)
                     /gene="yobF"
                     /locus_tag="ID725_RS07970"
                     /old_locus_tag="pgaptmp_001594"
     CDS             complement(53768..53911)
                     /gene="yobF"
                     /locus_tag="ID725_RS07970"
                     /old_locus_tag="pgaptmp_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGV7850223.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT        13664 a        13683 c        12890 g        14027 t
ORIGIN      
        1 gttaataaca gactaaagct tataatacca aatatacgat aatgaaaccg ggccaaaggc
       61 aagcggtcca gtcttgcgcc aatttgatca tattgttcca ttgggtgcct cggatttgct
      121 aaaaaaggta gcaattgcgg caaattcact tgccgcatct ttatcatcat tttatttcca
      181 ggcgcaggaa caaataatat tgacaacgcg tgcttaaatt accacaccag ctggatatta
      241 ttgtctttgc aaaatgctat ccactctgca cccggtgatt tatcagttat tatgtaatta
      301 atatgactaa agtcggctaa ctggacaaaa gctttgcgat caaacttaga gtgatcaacc
      361 aataacgcaa cttctgtcgc ctgacggatc attgtctttt tgatttcagc ttccgcttca
      421 ttagagtcca gcgcgccgct gttaatatca agacctttac aactcattac catgatatcg
      481 acatgatagc ggctgatgat ctctttagta attcttcctt gcagtgaaag tgtatttttg
      541 tttagttccc cacccgttga aacgacttta atttctgact gagccagaac atgaatcgct
      601 tccgccgagt tggttagcaa cgtcaggtca ctacggtcct gtaacaattt gagcaattcc
      661 attacggtac tactggaatc ggctgccatg gtggttttat tgtcgataaa gggtagtgcc
      721 ttgcgtgcaa taagctgctt ctcttcataa aacgatgaag cgcgcttata aaaatggata
      781 ttctccgtca acatcgctgt atttaaaaca gcaccaccat aggttctggt caaaaagcct
      841 tcatcttcca gcttctcaag atcgcggcga atggtttctt cggttacctg aaaaatccca
      901 ctcaaatttg agactgtcac ctttttatcg ttggcaacca tttgcttaat tgcctgaatc
      961 ctgtcttttg ccgccacgat tacacccctg tatcttttta catcacatta gcgcgattat
     1021 cgcataaccg atgtttactt tcaaaataac ctgtttgaat cacagatttt catcacagtt
     1081 ttcacagaaa cagaggtgaa tcgtgttgag tatttaacgc cccaagacct ctgccatttc
     1141 cctcatcaat gttgcgtctg catccgataa gttgatattc agtgccgcga cattttcgcg
     1201 tacctgttcc ggtgcagtag ccccactaag aatggagatt aaatcactct gttttaatat
     1261 ccacgccagt gccagagtgg gaattgtgca ctgataacga gcacaaagtg gctgccactg
     1321 ttcaagcata tcaatcactt tcagcatgtt ttcacgctgg aaccagactt tatttgcccg
     1381 agcgccgccc ggaacgtaat cacgagtgat ggtgccggtc aacaatccct gctctagcgg
     1441 ggaataaacc tgaaccacaa tgccattatc acgacatagc ggcagcagtt cgttttccat
     1501 tgcccggtcg aggatactgt atttcgcctg aataatatcc agttcaccat attgcagata
     1561 ctcgcggata tggtcagcat cgacgttagc agcgcctata gcgcgaattt tcccttcagc
     1621 ctttaactca ttaagcactg cgacagtttc agcgatcggc gtataaaatg gcggcaccga
     1681 ctgccagtgc gtcatgtaga tatcgatgta atcaaaaccc agacgttgca agctggcttc
     1741 tacctcttcg cggatagatt ccggggaaag gtttttatac aactgccgat cgccaacttt
     1801 gttgaataaa cttccttttc gttcccagac aatgccgcat ttggtttcta ctacaacctg
     1861 ttcacggggc agttttttta acgcctgacc gacgataact tcactattgc caaagttata
     1921 tcctggtgca gtatcaatca ggttaatgcc gcaacgatgg gcttcaagaa tcgtatcaat
     1981 acatatttgc cgatcgagat cgccattcca tgcaggaccg ccgccaatgg cccatgtccc
     2041 caaccccatt cgcgaaagcg taatatccgt tgtgcctaaa ggtatctttt tcatttgcct
     2101 tcattatcct tcgtattctt ccagcaattg ttcgaccagc tttctgtttt ttacgccggt
     2161 ggtggcaccg acgcttagaa ccgagatagc cgccgttgca ttggcaaagc gtgcgcattc
     2221 acgcagattt tttccttcta acagtgccgc aataaaacct gaagcaaagt tatcgcccgc
     2281 gccaatggtg tcgatggcgg ttattcctgc gaccgccggc accttcatcg tcatgtcacc
     2341 acgcttgata aagcagccgt ctttacccgt tttaatcacc accgttttta cgccgcacgc
     2401 aagaaagctg tcagcaattt catccagtgt ctctttcccg gtgagtaatt ttgcctcggc
     2461 ataattgaca taactcaatg cttcgcaaat atcatccagc gtttcattca accgcggttt
     2521 aatcatatcg gcacagatat tcatctgccg ggctttagct tgcgtaaaaa tttctgttag
     2581 cgctttacca tccaatagtg gactgttgaa aatactggcc agtgataata atttcgcctg
     2641 ggaaaaccgc gcaaaatcaa catcgtcaat attcaatttc cacagactgc cgttacggtt
     2701 tgtgacaaac gtccgctcgc catcctccgt caccagtcca acattaatag aggtatctat
     2761 gttgacgtcc tgcttcaggc tttgaatatc aatattctct ttgcggcaat ggtcgagaat
     2821 aaactgccct gcggcatctt taccaatacg gctcattaat gctgtacgat ggcccagacg
     2881 agaaataatt gttgcttcat ttattgcatc acccccggtg gtcattgcga ttctttcaag
     2941 aggataagaa tccacatcaa agatattttt actgaccggt tgcaatggaa tatcaacaat
     3001 agcggcacct atacaaataa cgtcgagatt atccatatca ttattccgct ttgccatcag
     3061 aaccgaacag tttaattttt tccaatgccc gttcttttac agctttacgt acttcacgtt
     3121 ctaaatgcag gaaaggctga tcctggtttt ctttcactgc gaccattgcg gcctgacaga
     3181 gctccgtatg gatgttgatt ttcgcgatac ccaacgaaat tgcagtttta atgtcggcat
     3241 cactgatccc cgatgcgcca tgcaaaacca gcggaacaga cactgcatcg cgtacgcgtt
     3301 tgacgacctc aaagttcaat tgtggctctg acgtataaac cccatgctgg ttgccgatgg
     3361 cgacagccaa tgagtcacag cccgtacgtt caacaaattc agccgcctgg tcaggatcgg
     3421 tatagtgata gcccgccagc gcttcctcat aaacggtttc attaccgaca tgccctaatt
     3481 ccgcctctac cggaataccc agcggatgga agaaatcaac agcctctttg gttaaacgaa
     3541 tattttcttc gaaatcaaat gcggaagcat cacgcattaa tgaattcata ccatgagtcc
     3601 aggcgttatg aataatctcc atactccgac catgatccca atgagttatt accggcaccg
     3661 ttgctttttg tgccatagat accatcatgt gagagaaatc ttcaaatgag gtgttgccga
     3721 caaaacctgt gccaaaagaa ataataaccg gtgatttcgc ttcttcggct gcgtcgataa
     3781 cgcccatcaa catttctgca ttccatacat taaaatgggc aatagcataa cgtttatttg
     3841 tggcatcgtt ttcccaatat ctgatatctg cgagcatgtt ctgttctccc gtattaatta
     3901 atcgctaatc ttaataacgc ctttaataat gtcgcgtttg ttgttaactg actcttcaaa
     3961 tgcctgttgt acatcccgat aatcgtaaat atgcgtcacc atcgatttca catcgaatcg
     4021 ccctgaagaa attgcttcaa tcgtgaccgg ataacgattg gcatagcgga ataccgtctg
     4081 gatagtgact tcgcgattga ttttgaggaa attgattgcc gaagcgccgg gtacagtacc
     4141 aacaatcata attttaccgc cacgcattac cagataaggt gcctgtttaa cggtgaccgc
     4201 agaacccgct gtttcgaaaa caatatctgc gcccatgtct tcggtaaatt gctgacagcg
     4261 tgcaatagtg tcttcttttg cgccgttaat aaccactgtc gcaccaagct gttccgccat
     4321 tgccagacgt ttttccagca catcaacgac ggcaatttcc gttgctccca ggcatttgca
     4381 cgcttgcaac gtcatcaaac caatacaacc tgctcccaga ataattatct tcttacccgg
     4441 tttaacatct gccagcatcg cggcatgcat cccgactgcg gcaggctcca ccagcgtccc
     4501 ttccatcgtg tccatattgt cgggcagttt gtaagtaaag ctctccggat gacacagata
     4561 gtgcgttaat gcgccgcggt agttgggttg tgtcgccata aaatcaacgt ccgggcagat
     4621 gttatatttg ccttccagac agtaacgaca gtgaccgcaa ggaacgccag gttcgatatt
     4681 tacccgatcc cccggtttaa atttgcgcac acggcttccc acagccacaa ccgtcccggc
     4741 gcattcatga cccaggccaa tttcttgatt tgggtcttta ggcggaataa acggacctga
     4801 ttcaaaacca tgtacatctg aaccacaaat accgacatat tctactttaa tcaaaacttc
     4861 atcttcttta ggcactggta tttctgctga aataattttc attgtgcccg gcacctgcaa
     4921 tattgctttt gaatttttca ttttgaatcc acccgtatta tttattggct actgcatcaa
     4981 tacttaaact ttcaacggag acacctttgg tttcgattcc aatggtggcg atagcaattg
     5041 cgacaataat tgaaaccgcc cccagaagaa taaagactcc cgtaacgcca taactactga
     5101 gcagcactgc aacggcataa ggtgcggcaa taccactgat tcgccctacc gcattcgcca
     5161 gaccggagcc acggagtttg gcctctgtcg gccagatctc agggacatac actgccgagg
     5221 catagcaaac gtacatatag acgaaagtaa tcaggaagaa accaatcaac gttatgagca
     5281 acatgctggt ttgcagtgaa tagatatatc cgagcaccgc aatcaggatt aatagcccca
     5341 cacccattgt tttacgcgga attttatcca tcaccagcat ggcaataaaa atgccaaatg
     5401 gcgtgccaaa catactcatg gtatttaaaa caatcgagtc ttttaaatta atcccctggg
     5461 tcatgaatat cgttggcaac cagttaatta gtgtgtactg cacaacgttc atggcaatca
     5521 gcacacaaga gcctaatatc acgcgtttca gtaatactcc tgtcagtaag gctgaatatg
     5581 gcaccgcttg tggtgcttta ccgtcatcag caataaccac gggcggcaaa ggtttacctg
     5641 tatggcgtat gacgccttct tctatactcc gcatgacttt ctcggcttcc tgataccgtc
     5701 cgcgcgattc cagccagcgc ggggattcag gaaagtagcg ccaggccagc gccgtagcga
     5761 taagcgacaa tattgcaggg ataagcagtt gtacccgcca gttccactct gcactaatca
     5821 gcggcgtgag tcccatcgct atcaatgaac agagcggata tgaccagttg ccaataaagg
     5881 aaacccgact tgaccacgtt ccacgatttc taccgggcat atattcggtg aaaccagcaa
     5941 acagcgtaac cagtaaagct cccagcccaa ctcccatcac aaaacggcag gcaataagga
     6001 aatccatatt cggtgagaaa gcaccgacaa ccatcgaggc aatatgaatg gcctcataga
     6061 ggatgaacgc atttctgcga ccggttttgt cgccaatgat gccgcccacc agcgcaccga
     6121 gaaacattcc ggcggtcgtg attgctgaga atgtggctgt ggtggaatta tctgtccagc
     6181 ccaacgcttt cagctgcgcg aggatcaagc caccaacggc attactccag cagacaagca
     6241 agccaaacgc gacgatggca aacattgatg aatgccagcg gcaatccggt aagcgatcca
     6301 gccgtgcacc acaatgcggt tttgttatct gatccatgcg ataacgttcc ttcaggagag
     6361 tggttattca tcaaagtcgt aagtcatgat cactttgatt gcggttttat cgaccatcgc
     6421 atcaaaccct tcgcgccatt gcgacaggcc gatacgatgc gtgatcatcg gtttgacttt
     6481 gatagcgccg ctggctaata gcctgatagc gttacgccat gaggtggagt cataggccat
     6541 atgcccaatg atgcttttgt tccaggcggt aatgtcatta atcgagaaat caagaggttt
     6601 gaagcccatt ccaacgcgta ccacttcccc gttcggacgg agcatttcga tggcttgttt
     6661 cagtgcgata ttggcaccgg agcattcaat caccagtccc agattgtctt tgccacaaat
     6721 ttgctggcag cgcgccacca catcttcggt agaaccattt actactgccg tcgcacccag
     6781 ttcttttgca accgggaagc ggaccgccac atcttcttgc agaccaacga cgacgatatt
     6841 taccgccccc ataattcgcg ccatttgtac ggagaacagc ccgagtgggc cagtgccgat
     6901 gacgaccaca tcctgaccag ggaggaattt cgattgctgc gcgatggatt tgtaggcatt
     6961 acagataggg tcaagtacgg ctgcgtcctc ataatcaaca ccatcaggga tttcccacaa
     7021 cgcatgacga tgaattttga gaatttcacc aggaaccaga caatatttgg aaaaaccacc
     7081 gccccaggta ttgttatcca gaccaaggtt tactttttct gtacaacaca gaaaatcacc
     7141 ttgttcacag gccggacaaa cgccgcagac gtgaccgctg ttatccgaca cgacgcgttg
     7201 ccccactttc cagtctttga ctttttcacc aacctgcgca atacaacctg cgaactcatg
     7261 gccgcggata gagttaaact catcagaacc gctatcgaca ttgtagtgct tcatgtctgc
     7321 gccgcagatt gccgcggctt taatttcaat cactacatct tccgggccac aaatgggttg
     7381 tggcacatcg atcatcttgt agccgccaaa ggctttgcca aaccgagcca gtgctttcat
     7441 tgcgtcatcc tctgtgtttt gttttgctta gatggtcatc atacttaccc acatgatttt
     7501 tgaggtcaac attaaacatg taaaacacag ataataatct gcgttttaca actcagatca
     7561 caattacgca gaaaatggct ggttctgtta caggttgatg taaggcggaa cgcaaaagga
     7621 gcaaatttga gagggattac cggccaaata ggtctgagac ttaagttcag gagaagtcat
     7681 tcaggcgtga acgcgacggc aagagatcgg actgggactt agccaggtca tggcgagata
     7741 attgagatga gtcgtggggt cattgttggc tatccccttc atagccgcgg caaaaagggg
     7801 atgaaccact tacttaaaca tggcaatagg ctttgctgag ataccaaaat cttcttttaa
     7861 ctgctgtttg ctcttcatga ccatctgacc atttgtgtca attgtcatat gctgtgcttc
     7921 ggtattattg cgggcttgcc acagcatcac cagttgcagg cagttctctt tttgttcctc
     7981 ggtcaacgca acgccatcag gccatttccc cagttcaacg gcggtcgaca aacgctggta
     8041 tacctcaggc gtcatgctgt tgataatgtc atcaagattc ataatttttc cgctcctgtg
     8101 gaataagttg ctgaatcgtt ttttcaaccg ttgatttctt cgccgttttc accatcagtg
     8161 aagcgtaaag aagcagagtt aacacaataa cgttcgcccg ttggctgcgg cccgtcaggg
     8221 aagacatgcc ccagatgggc atcacagtta ccgcaacgaa tttctatgcg ctgcattcca
     8281 tgtgacaagt ctttgatata acgaatggat tcttcactca ccggttcgta gaaactgggc
     8341 cagccacagc cggaatcata cttggtttgg gaatgaaaca gcggggcatc gcagatcaaa
     8401 cagtgatata cgccgtcacg cttgttatgc agtaaacgac ccgtaaatgg cggctctgtc
     8461 ccatgattct gcgtcacgta aaactgcatc tcggacaaat tttttttcag ttcttctgcc
     8521 gaaggtttat tagccatttg ctcacatctc actttaatcg tgctcacatt acgtgactga
     8581 ttctaacaaa acattaacac caactggcaa aattttgtcc taaacttgat ctcgacgaaa
     8641 tggctgcacc taaatcgtga tgaaaatcac atttttatcg taattgccct ttaaaattcg
     8701 gggcgccgac cccatgtggt ctcaagccca aaggaagagt gaggcgagtc agtcgcgtaa
     8761 tgcttaggca caggattgat ttgtcgcaat gattgacacg attccgcttg acgctgcgta
     8821 aggtttttgt aattttacag gcaacctttt attcactaac aaatagctgg tggaatatat
     8881 gactatcaaa gtaggtatca acggttttgg ccgtatcggt cgcattgttt tccgtgctgc
     8941 tcagaaacgt tctgacatcg agatcgttgc aatcaacgac ctgttagacg ctgattacat
     9001 ggcatacatg ctgaaatatg actccactca cggccgtttc gacggtaccg ttgaagtgaa
     9061 agacggtcat ctgatcgtta acggtaaaaa aatccgtgtt accgctgaac gtgatccggc
     9121 taacctgaaa tgggacgaag ttggtgttga cgttgtcgct gaagcaactg gtctgttcct
     9181 gactgacgaa actgctcgta aacacatcac cgctggtgcg aagaaagtgg ttatgactgg
     9241 tccgtctaaa gacaacactc cgatgttcgt taaaggcgct aacttcgaca aatatgctgg
     9301 ccaggacatc gtttccaacg cttcctgcac caccaactgc ctggctccgc tggctaaagt
     9361 tatcaacgat aacttcggca tcatcgaagg tctgatgacc accgttcacg ctactaccgc
     9421 tactcagaaa accgttgatg gcccgtctca caaagactgg cgcggcggcc gcggcgcttc
     9481 ccagaacatc atcccgtcct ctaccggtgc tgctaaagct gtaggtaaag tactgccaga
     9541 actgaatggc aaactgactg gtatggcgtt ccgcgttccg accccgaacg tatccgtagt
     9601 tgacctgacc gttcgtctgg aaaaagctgc aacttacgag cagatcaaag ctgccgttaa
     9661 agctgctgct gaaggcgaaa tgaaaggcgt tctgggctac accgaagatg acgtagtatc
     9721 taccgatttc aacggcgaag tttgcacttc cgtgttcgat gctaaagctg gtatcgctct
     9781 gaacgacaac ttcgtgaaac tggtatcctg gtacgacaac gaaaccggtt actccaacaa
     9841 agttctggac ctgatcgctc acatctctaa ataagttgag atgacactgt gatctaaaaa
     9901 gagcgacttc ggtcgctctt ttttttacct gataaaatga agttaaagga ctgcgtcatg
     9961 attaagaaaa tttttgccct tccggtcatc gaacaaatct cccctgtcct ctcccgtcgt
    10021 aaactggatg aactggacct cattgtagtc gatcatcccc aggtaaaagc ctcttttgca
    10081 ttacagggcg cacaccttct ctcgtggaaa cctgcaggtg aagaagaagt tctgtggttg
    10141 agcaacaaca caccgttcaa aaatggcgtt gctattcgcg gtggcgtacc ggtttgctgg
    10201 ccgtggtttg gtccggcggc acaacaaggc ctgcctgcgc acggttttgc ccgcaacctg
    10261 ccgtggacgc tgaaatcaca tcgtgaagat gctaatggcg tagcgctgac ttttgaattg
    10321 acacaaagcg aagagacgaa aaaattctgg ctgcacgact ttactctgtt agcgcatttc
    10381 cgcgtggata aaaactgtga aatcgatctt gaagcacacg gcgaatttga aaccacctct
    10441 gccctgcata cctactttaa cgtgggtgat atcgctaagg taagcgtcag tgggctgggc
    10501 gatcgcttca ttgataaagt aaatgacgcg aaagaagatg tactgaccga cggtattcag
    10561 accttccctg accgtaccga tcgcgtgtat ctgaatccgc aagattgcag cgtgattaat
    10621 gatgaagcgc tgaatcgtat tatcgccgta ggccaccagc atcatctgaa cgttgtaggc
    10681 tggaacccgg gaccggcgct ttcagttagc atgggcgaca tgccggacga tggctacaag
    10741 acctttgttt gtgtagaaac agcttacgcc tcagaaatgc aaaaagtgac caaagaaaaa
    10801 cctgcacatc tggcgcaatc cattcgcgtt gccaaacgtt aatttgcgtc aatgttgtgt
    10861 gccgggtgcg atgcatccgg cacgtatcaa catcacacca tatccagcgc agtttttcct
    10921 tttggtgccg gatacgcttt gtccagcatg gctaattccg ctgacgaaag ttcgacctca
    10981 agcacagccg cattctgttg gacatgggca atcgtggccg cttttggaat cgccatcaca
    11041 ccctgatggc tgattaccca cgccaacaat acttgcgccg cgctgatatt gtgagcatgt
    11101 gcaatttcgt tgactaccgc gttttttaac agtccattgc gcaaccgccc ggcctgggct
    11161 aacggactgt aagccatcac cggcatctgc tgttgctggc accaggggag tagatcgtac
    11221 tcaatccctc gtgaaccgag atggtaaagc acctgattag tggcacactg atttcccccc
    11281 ggcagctgcc agagttcctg catatcagca taatcaaggt tcgaaacgcc ccagcggcgg
    11341 atttttccct gggcgatcaa tttttccatc actgcaacag tctcttcaaa agcgaaactg
    11401 ccagaccagt gtaataagta gagatcgaga taatcagtat tgagacggcg taaactggct
    11461 tcgcatgcat ttatcgcttt ttgcccgcca gcattccacg gatagacttt agagacgaga
    11521 aagaccttct ctcgcagacc ggttaatgct tccccaacca ccttttcggc accgccatcg
    11581 gcatacattt cggcggtatc aatgagggtt aaaccgagtt caatgcccgc gcgtagtgca
    11641 gcaacttctg ttttgcgctg actggcatct tcgcccatat accatgttcc ctgccctatg
    11701 gctggcagtg agacatcgcc actaaattga atcatttttt gttgcattgt ttcctcccag
    11761 gtattgcacc accgtaatgc aaaacagggc gtgacgccct gtttttatgc acaaaatgcc
    11821 ctggaaagat gcattatcag aatttgtagg tgatcccggt agaaatcagg ccagtccagg
    11881 atttatccac aatcgggctg tcagtaactt catcagacag acgggtgtag cgcgcggtac
    11941 cgtaaacact ccagtcgccg aggaagttgt agctggcgct cagctccagg taagggctcc
    12001 agctgtcgtt cgagttatag ccacgcagac cgctgcgagc ggactctttg cgcgatacgc
    12061 cataatagta ttcgttctgg ttttcgctgt tccactgcac accaataccc ggagtcacgg
    12121 tcaggccacc gttggtgtaa cgatacaacc aggccatatc ccagacgatg ccgttgctgt
    12181 tatccagggt atcgccagcc agggtggtac gcaggtaacc gtactgggta aagtgagcat
    12241 aagacagacc agccatcatg gtgctcttac ggtcatccag gtgacgcatt tggtgatcgc
    12301 cactgtcttt agctttgaag taaagcggcg accagtaagc ggtaattgaa agtttatccg
    12361 ttgcgtcatt ccacaggtag taaccaccac ctaagccacg gaaccagaag ttatcgcctt
    12421 catagttgat taccggtact gggtaaacat cggtatcgta atctttatat gggtgttcaa
    12481 cgacacctac gcctgcgccc agggaaaatt taccttcagc gtgcgctacg cctgcagacg
    12541 ttgcgataag cactccaagt gccagaagtt tgagtttggt cacaattaat cattccttaa
    12601 acaaatgttt agcgggcgac aaagtttacc cgtcaataca tcgaaaccca acctttttac
    12661 gttttcattt tttaaagtaa ctgtttaatt ttcctgacgc ggatgacacc gcgcttacag
    12721 ccaaatgaat ttagcgttac tggcgagcct ggtctttaca ttaattatgc aaaatttatg
    12781 gatgagttgt tgatatgcca ttgaaattaa gaaagccgtg taggcaagtt ttccatttgc
    12841 catctacgct taattttgaa ggtgtatcac cgggcacgtt gttctcatcg tcgataaaat
    12901 ggcatgagag ttgctgtgtt ttagcaagag acgtcgttca gtttacctct tccgggagcc
    12961 tctactattc atatgaacgg ctcttaacct gtgctaaaaa acgaaaggac ggcataccat
    13021 gaatatattc gatcactatc gccagcgata tgaagctgcc aaggacgaag agttcacact
    13081 gcaggagttt cttaccactt gtcggcaaga tcgcagtgct tatgccaacg cggctgagcg
    13141 gctattgatg gctatcggtg agcctgtcat ggtcgataca gcccaggaac ccagactttc
    13201 tcgactcttt tctaaccggg tcattgcacg ttatccggcg tttgaagagt tttacggcat
    13261 ggaagacgcg attgaacaga ttgtctctta tctgaaacac gcggctcagg ggctggaaga
    13321 gaagaaacaa atcctgtatc tgctggggcc tgtgggtggg ggtaaatcat cgcttgctga
    13381 gcgactgaaa tcattaatgc agctcgtacc gatttatgta ttgagcgcca acggtgagcg
    13441 tagcccggtc aacgatcatc cgttctgtct tttcaatccg caggaagatg cgcagattct
    13501 ggaaaaagag tatggcattc ctcgccgtta tctcggcacc atcatgtcgc cgtgggcggc
    13561 aaaacgcctg catgaatttg gtggcgatat cactaagttc cgggtagtga aggtctggcc
    13621 gtcaattctg caacaaattg ctatcgccaa aacggaaccc ggtgatgaga acaaccagga
    13681 catctccgcg ctggttggga aagtcgatat tcgtaaactc gaacattacg cgcagaatga
    13741 cccggacgcc tacggctatt ccggtgcgct gtgccgcgcc aaccaaggga tcatggaatt
    13801 tgtcgagatg tttaaagcac cgataaaagt gctgcatccc ttgttaaccg ccactcagga
    13861 aggtaactac aacgggacgg aaggtatctc cgccctgccg ttcaacggga ttattcttgc
    13921 ccactcgaac gagtccgaat gggtcacttt ccgtaataac aaaaacaacg aagccttcct
    13981 cgatcgtgtt tacatcgtga aggtgccgta ttgcttgcgc atttccgaag agatcaaaat
    14041 ctacgagaaa ttgcttaatc acagtgaatt gactcacgcc ccatgcgccc ctggcactct
    14101 cgaaacactg tcacgttttt ccattctttc gcgcctgaaa gagccagaaa actccagcat
    14161 ttattcaaag atgcgggttt atgatggcga aagtctgaaa gacaccgatc ccaaagccaa
    14221 gtcgtatcag gaatatcgtg actacgccgg tgtcgatgaa ggtatgaacg gtctgtcgac
    14281 gcgttttgcg tttaagatcc tctcccgcgt gttcaacttc gatcatgtag aagtggcggc
    14341 aaacccggtc catctgttct acgtcctgga acagcagatc gagcgcgagc agttcccaca
    14401 agagcaggca gaacgctatc tggagttcct gaaaggttat ctgatcccga aatatgccga
    14461 gtttattggc aaagagatcc agacggccta ccttgaatcc tattccgaat atgggcaaaa
    14521 cattttcgac cgttatgtta cctacgcgga tttctggatt caggatcagg agtatcgcga
    14581 tccggatacc gggcagctgt ttgaccgcga gtctcttaac gccgagctgg agaaaatcga
    14641 gaaaccggcg gggatcagta atccaaaaga tttccgcaac gagattgtta acttcgtact
    14701 gcgcgccaga gccaataaca gcggacgcaa tccgaactgg accagctatg aaaaactgcg
    14761 cacggttatc gagaagaaaa tgttctccaa taccgaggag ctgttgccgg ttatctcgtt
    14821 taacgccaaa acgtcaaccg acgagcagaa gaaacacgac gactttgtcg accgtatgat
    14881 ggaaaaaggc tacacccgta aacaggtgcg tttactgtgc gaatggtatt tgcgcgtacg
    14941 taaatcgtct taacaaccct ggcccggtac acatgctacc gggcctacaa cgacagcgaa
    15001 ccgtgggcca gagaaatggc aacacaggcg tagcatacag ttggcaaatg tagtacgggg
    15061 ggcatatgac ctggtttatt gaccggcgtc tgaacggcaa aaacaaaagc atggtgaatc
    15121 gccagcgttt tttacgccgt tataaagcgc aaattaaaca gtcgatctcc gaggccatta
    15181 ataagcgttc ggtgactgac gtcgacagcg gcgagtccgt atccattccc acggaagata
    15241 ttagcgaacc aatgtttcat caggggcgtg gcggtctgcg ccaccgcgtg catccgggca
    15301 atgaccattt cgttcagaac gaccgaattg aacgtcccca gggtggcggc ggaggttccg
    15361 gcagtggtca gggccaggcc agccaggatg gtgaaggtca ggatgaattt gtctttcaga
    15421 tttcgaaaga tgagtatctt gatctgctct ttgaagattt ggccttaccg aatctgaaac
    15481 aaaaccaaca acgccagctg accgaatata aaacgcatcg ggcgggttat accgctaacg
    15541 gcgttccggc caatatcagc gttgtgcgtt ccttgcagaa ctcactggcg cgacgcacag
    15601 ccatgacggc aggcaagcgg cgggaacttc atgcactgga agagaatttg gccatcatca
    15661 gcaacagtga acctgcgcag ctgctggaag aggaacgtct gcgcaaagaa attgcagaat
    15721 tacgtgccaa aattgaacgc gtccctttta ttgacacctt cgatttacgt tacaagaact
    15781 acgagaagcg gcccgatccc tccagccagg cagtgatgtt ttgcctgatg gacgtttccg
    15841 gttcaatgga tcaatccact aaagatatgg ctaagcgttt ttatattctg ctgtatctgt
    15901 tcctcagcag aacgtataag aacgtggaag tcgtatacat ccgccatcat acccaggcga
    15961 aagaagtcga tgaacatgag tttttctact cgcaggaaac tggcggcacc attgtttcca
    16021 gcgccctgaa actgatggat gaggtagtga aagagcgtta taacccggca cagtggaata
    16081 tttacgctgc acaagcatcg gacggcgata actgggccga tgactctccg ctttgccatg
    16141 aaattctggc gaaaaaatta ttacccgttg ttcgttatta cagctatatc gaaattaccc
    16201 gtcgcgcgca tcagacactg tggcgagaat atgagcatct gcaatctact ttcgacaact
    16261 ttgcgatgca gcatatccgc gaccaggatg atatttatcc ggtgttccgt gaactgtttc
    16321 ataagcaaaa tgcaacagct aaagactaaa actatcagcc gggtcattat cgcctggctg
    16381 atttttagct cactgtaagt tatctcattt attacataca ttagcttaca atcgctttaa
    16441 atatgacagc ataaccttta cacaatttag ttccagaaaa caatcattcg gaaaaattat
    16501 tctgtcaaca cgtatttccc tggggttatt ctttaaatat tttttatcgt taacgaaaat
    16561 tgatcctggt caaaactata tatctctgcc atcaataaaa tccagcactc acattgctct
    16621 cctttttatg gtttctatgg gtacacaaaa attaaaagct caaagctttt ttattttcag
    16681 tttattgctg acgttaattt tattttgcat tactacctta tataacgaaa acacaaatgt
    16741 aaaactcatc ccacagatga attacctgat ggttgttgtg gctttgtttt tccttaacgc
    16801 cgtcattttt cttttcatgt taatgaaata tttcactaac aaacaaattt taccaacact
    16861 cattttaagc cttgcatttt taagtggcct tatctattta gttgaaacca ttgtaattat
    16921 ccataaacca attaacggca gtacactgat ccagacaaag tcgaatgatg tttctatttt
    16981 ctatattttc cgccaactca gttttatttg tttaacctcg ctggcgctct tttgttatgg
    17041 aaaagacaac atccttgaca acaataagaa aaaaactggc attctgttgc tggcgctgat
    17101 ccctttttta gtttttcccc ttctggcaca caatctgagc agttataacg ctgactattc
    17161 tttgtatgtc gtcgattact gtccggacaa tcatactgcg acctggggaa tcaactatac
    17221 aaaaatattg gtttgtctgt gggcattttt actgttcttt attatcatgc gtacacgatt
    17281 agccagcgaa ctatggccgt taatagcatt attatgtctg gcatcgctat gctgcaactt
    17341 acttctactg actctggatg agtataatta taccatctgg tatatcagtc gcgggattga
    17401 agtctccagt aaactgtttg ttgtgtcttt tctgatttat aacatttttc aggagctgca
    17461 actctccagc aaactggcag ttcatgatgt gctgaccaat atttataatc ggcgctactt
    17521 tttcaacagc gtagagtcat tattgtcgcg acctgttgtt aaggacttct gtgtcatgct
    17581 ggttgatatt aatcagttca aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa
    17641 agtgctggtt tcaattgtcg atattatcca gcaaagcatc caccccgatg atattttagc
    17701 gcgactggag ggtgaggtgt ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa
    17761 aatcattgcg gaacgtatgc gtaaaaatgt cgaactcctg accggcttta gtaacagata
    17821 tgatgttcct gaacaaatga ccatcagtat tggcacagtt ttttcaacgg gtgacacgcg
    17881 taatatctcg cttgtcatga cggaagcaga taaagcctta cgcgaagcga aaagcgaggg
    17941 gggcaacaaa gtgattattc atcatattta agtgcaaaaa tattcagagc catgcttttt
    18001 gcgtggcttt tgcatacaat ttattactat tacccctaaa tttcccctcc ccagcggcgc
    18061 agagatgagt ataattagcg cccctgtgcc aggccgcaat cgaactttat ctggttttct
    18121 cgtttcacta accgaaggag tgccatttat catgaaattg caccatagaa tgctccggca
    18181 ttttatcgcc gcaagtgtca ttgtgctgac atcttccttc cttatttttg aacttgtcgc
    18241 cagcgacaga gcaatgagtg cctatctgcg ctatatcgtg cagaaagcag actcctcctt
    18301 tctttatgat aagtatcaga atcagagtat tgccgcgcat gtgatgcgcg ctctcgctgc
    18361 tgagcagtcg gaagtgtcgc cagaacagcg gcgcgccatc tgcgaggctt ttgagtctgc
    18421 caataatacc catggcttaa acctgactgc ccataaatac ccgggcttac gcggcacatt
    18481 acaaaccgca tccactgact gcgacacaat tgtggaagct gcagcactat tacccgcttt
    18541 tgatcaggca gtggaaggca accgccacca ggatgattac ggttcaggtc ttgggatggc
    18601 cgaagagaaa tttcactatt atctcgatct caatgaccgc tatgtctatt tttatgagcc
    18661 ggttaatgtt gaatactttg cgatgaataa ctggtccatc ctgcagtcag gaagtattgg
    18721 catcgatcgc aaagatattg aaaaggtatt taccgggcgt accgtattgt cgagcattta
    18781 ccaggatcag cgtactaaac agaacgtgat gagtttgctg acgccggtgt atgttgcagg
    18841 gcagctaaaa gggattgtgc tgctggatat taacaaaaac aatctgcgga atatctttta
    18901 cactcatgac cgccctctcc tctggcgttt tctcaatgtc acgctaaccg atacagattc
    18961 ggggcgcgac attatcatca accagagcga agataatctg ttccagtatg tcagttacgt
    19021 ccatgactta ccgggcggca ttcgtgtctc gttatccatt gatattcttt actttatcac
    19081 gtcttcgtgg aaaagcgttc tgttctggat tttgacggcg ttaattttgc tgaatatggt
    19141 gcggatgcac ttccgcttat accaaaatgt gtcgcgagaa aatattagtg atgcgatgac
    19201 tggactgtat aatcgcaaaa ttttaacccc tgaactggag cagcggttgc agaaactggt
    19261 gcaatccggt tcttcggtga tgtttattgc aattgacatg gacaagttaa agctaataaa
    19321 tgacaccctc ggtcatcagg aaggggattt agcgattacg ttgttagctc aggcgattaa
    19381 acaatcgatt cgtaaaagtg attatgccat ccgactcggt ggcgatgaat tctgcatcat
    19441 tcttgtcgat tcgacgccgc aaattgcagc acaactgcct gaacgtatcg aaaaacgtct
    19501 gcaacatatc gcgccgcaga aagagatcgg cttctcttcc ggtatttacg cgatgaaaga
    19561 aaacgatacg ttacatgatg cgtataaagc ttccgatgag cgtttatatg tcaataagca
    19621 gaacaaaaac agccgttcat gataaccttc tgtggttgtt tgcttgtaat ctcaggagcg
    19681 tgaaatgact gaaatggcta aaggaagcgt gacgcatcag cggttaatcg ctttattatc
    19741 acaagaaggt gctgatttcc gcgtcgttac ccatgaagcg gtaggtaaat gtgaagcggt
    19801 gtcagaaatt cgtggcaccg ctctaggcca gggtgctaag gcactggtat gtaaagtcaa
    19861 aggcaatggc gtaaatcaac atgttctggc gatcctcgca gccgatcaac aggccgatct
    19921 gagccaactc gccagccata taggcggatt acgcgcttcg ttggccagcc cggcggaagt
    19981 cgatgaactc acaggctgtg tcttcggcgc gatcccccct ttcagcttcc atccaaaact
    20041 caaactggtt gccgaccctc tactcttcga acgatttgat gaaatcgcct tcaatgcagg
    20101 gatgctggat aaatccgtta ttttgaaaac cgcggattat ttgcgcattg cgcaaccaga
    20161 actggtcaat ttccgccgca ctgcgtaact agcctgtccg ttcaataagc agaacggaca
    20221 aaaccagcac aacagcaatg gcaaaaaacg atgacgtgat aatcagtgtc tcgacgaaca
    20281 tttgatcgtt catagcatac ccccgggaga tagctgtttt cacgttattg ttagcgtgca
    20341 caaatggcag tttgatgaca gttcgcctga ttttttatgc aaaaaacgta aagattttct
    20401 acttccttcc tgcagcaagc gtaaagtaag caggcttatt attttttggc aaggaaacca
    20461 cgatgtttga tgtcactttg ctgatcctgc tcgggttagc tgcgctgggc tttatcagtc
    20521 ataacaccac tgtcgccgtt tcaattctgg tgttgatcat tgtccgcgtc acaccgttaa
    20581 gcaccttttt cccgtggatt gaaaaacagg ggctaagtat cgggataatc attctgacga
    20641 ttggcgttat ggcccccatc gccagcggga cgctaccgcc ctcaaccttg attcactcgt
    20701 ttctgaactg gaaatcgctg gtggcaattg cagtaggcgt tattgtctcc tggctgggtg
    20761 ggcgcggcgt gacgttaatg ggcagccagc cgcaactggt tgctgggctg ctggtgggca
    20821 ctgttttagg cgtagcgctg tttcgcggcg taccggttgg cccgcttatt gccacgggtc
    20881 tggtttcgct gattgtgggg aaacagtagt taatcccgca atatatcggc ctggcgtttg
    20941 ccccaggcct ttcttaaaca tagtaataaa ggccgttgtt gaatcatacc ccagcgtatg
    21001 agcaactttc tgtacagtat cgcctttcac taatccctga agcgccataa tcagctgtaa
    21061 ctgctgtcgc cactggcgaa aactcaaccc cgtctctttt actatcagtc gggcaagatt
    21121 gcgttcactc atcgcaaaaa agccagccca ttgccccaat gccccccact cgacgggctc
    21181 tttcgccatc atctccacca tcgcgcggat tttaggatga gaagaaacgg gtaggtgcag
    21241 ctgttgttgt ggctgctgag ggagttcatc aaacaggacc tgaattaatc tgcgggtcat
    21301 cggctcagca cgctgagtcg tcgtcctgtt tgccagagtt aaaattaatt cccggcataa
    21361 gggcgatatt ttcaatgtgt aacatgttgt cggcattgtc actgcgctgg gttcgataaa
    21421 cagaaaacag agttcagcgt tagctgtcac ttgattgcta tgttccaccc cgcccggtat
    21481 ccaaaccgca tattgtggcg gcaccatcca caaagcattt tccaccgtac aggtaattgc
    21541 accatgtagc gccagtatca gctgtccttt gcggtgctga tgtacaggac tggttaactc
    21601 atccgttccg gcgtgaatac aaaatgcgac cgcggcttca tggtgtcggt cgggttcata
    21661 gccattgaga ttcaacctgt gcatcatttt gtccgaattt agcgataatt tgtcatttta
    21721 gcttgattca acataacaat aaaaacggta aggtacagcc tcgtttgtaa caatgagaag
    21781 catatgacct gttcaacttc attaagcggc aaaaacagga ttgtccttat cgctggcatt
    21841 ctgatgattg ccactacatt acgcgtcacc ttcaccggcg cagcaccgtt actcgatacg
    21901 attcgttccg cttactcgct gacgactgcg caaaccggct tattgaccac cctgccatta
    21961 ttggcctttg cgctaatctc acctctggct gccccggtgg ctcgacgttt tggtatggaa
    22021 cgtagcctgt ttgccgcgtt acttttgatc tgtgcaggta tcgcaattcg ctctctccct
    22081 tcgccttact tattatttgg cggtacagcg gtcattggcg gtgggattgc attaggtaat
    22141 gtcttactgc caggattaat taaacgcgat ttccctcatt ccgtcgccag acttaccggc
    22201 gcatattccc tgacaatggg agctgcggcg gctctgggat cggctatggt agtgccgctg
    22261 gctttgaacg gttttggctg gcaaggcgcg ttgctcatgc tgatgtgttt tcctctgctg
    22321 gctctttttt tatggctgcc acagtggcga agccaacaac atgcaaattt gagtacttca
    22381 cgcgccttac atactcgggg tatctggcgt tcgccgcttg cctggcaggt aacattgttt
    22441 cttgggatca actcactggt ctattacgtg attattggct ggcttccggc gatcctcatc
    22501 agtcacggct atagcgaagc acaggcgggt tcactgcatg gtttgctgca actagccaca
    22561 gcagctcccg gtttgctgat cccacttttc ttacatcatg tgaaagacca gcgtggtatt
    22621 gcagcgttcg ttgccttgat gtgcgcagtg ggcgcggttg ggctctgctt catgccagcg
    22681 cacgcgatca cctggactct gcttttcggt tttggttccg gcgcaacaat gatactgggg
    22741 ttgacgttca ttggtctgcg ggctagttct gcgcatcagg cggcggcact ctcggggatg
    22801 gcacaatccg tcgggtattt gttggcagcc tgtgggccgc cgctgatggg taaaatacac
    22861 gatgctaacg gtgactggtc cgtaccacta atgggtgttg caatactttc actactgatg
    22921 gcgattttcg gactttgtgc cgggagagac aaagaaattc gctaatatcc ggtactatag
    22981 tgacgtgaca aatcatgcgt gaaagggaga acaaacacga tgaatattca gtgcaaacgc
    23041 gtttatgatc cggcagaaca gagcgatggt tatcgcgtac tggtcgaccg cctctggcca
    23101 cgcggtatca aaaaaaccga tttagccctt gatgagtggg ataaagaaat cacgccgtca
    23161 acggaactgc gcaaagcctt tcacggcgaa gtcgtcgatt ttgcaacctt tcgcgagcaa
    23221 tatcttgcag aactggcaca acacgagcaa gaaggaaagc ggctggcgga catcgccaaa
    23281 aaacagccgc tgaccctgct ctactcagca aaaaacacca cgcagaacca tgcgctggtg
    23341 ctggccgact ggctacgtag cttgtgattt tagtacagca tccggcggtt atttttcacc
    23401 agccggatgg tccagtcgcc acaatgccca ttcatcaatc gtttcaccgc ccggtaattt
    23461 gcaattgttg ctgacccctt gcgctgtctg cactggaatg agcgtcccgc ccttctgctg
    23521 gcaatagacc gacgccggat ttgccatacc aatctgcggc ggtttaggtg cttctggctg
    23581 agaaggggtt gaacaaccag ccaggaccag caagcaaggc agaacaaaac tgataatttt
    23641 catttattga tctcacatat gtatccaagc ttagagtatc gcggtatcgt tttgttttgc
    23701 agcactattt ttattacatt cactcaaaac atattacgtc ttgtttcatc tttgttgatg
    23761 atgttttatc atgcctgcaa agattaaata atcagcattt acccgccgta tcctggagtt
    23821 gtttcgtgtc agatcagatt atcgcccgca tctcgcaatc ccttgccaaa gaacagtcac
    23881 tggaaagtct ggtccgacag cttctggaga tgctggaaat ggtcactgat atggaatcaa
    23941 cctacctgac caaagtggat gtcgaagcgc gcctgcagca tattatgttt gcccgtaaca
    24001 gccagaaaat gcacatcccg gagaatttta ccgtctcgtg ggattactcg ttatgcaaac
    24061 gcgccattga tgaaaactgc tttttcagcg atgaagtccc tgaccgttgg ggcgactgta
    24121 ttgcggcacg caatcttggc atcaccacat ttctgagcac gccaattcac ttaccggatg
    24181 gatcattcta tggcacgctt tgcgccgcca gcagtgagaa gcgccagtgg agtgaacgcg
    24241 cggaacaggt tttacagtta ttcgccggac tgattgcaca atatattcaa aaagaggcac
    24301 tggttgaaca gctgcgcgaa gccaatgctg cgctgattgc gcaatcgtat accgactcgt
    24361 taaccgggct accgaatcgg cgggcgattt ttgaaaatct gacgacgctg ttttccctcg
    24421 cccggcatct taaccaaaag ataatgatcg cgtttatcga tctggataac ttcaaattaa
    24481 tcaatgatcg ttttggtcat aatagtggcg atctgtttct cattcaggtt ggcgagcgcc
    24541 ttaatacgct ccagcaaaat ggcgaagtta ttggtcgtct cggcggtgat gagtttttgg
    24601 ttgtttcact aaacaacgag aatgcggata tttcgtcgct gcgagaacgc attcagcagc
    24661 aaatacgcgg agaatatcac ttaggtgatg ttgatttgta ttatcctggt gccagtcttg
    24721 gcatagtaga agtcgatcct gaaacgaccg atgcagacag tgccctacat gctgccgata
    24781 tcgcgatgta tcaggagaaa aaacacaaac agaaaacacc ttttgtcgcg cattcagcgc
    24841 cacattcctg aggcgtattc acatcctttt gattggtgat aacatgcgaa tcggtattat
    24901 ttttccggtt gtaatcttca ttacagcggt cgtattttta gcatggtttt ttattggcgg
    24961 ctatgctgcc ccgggagcat aaagatgaaa aaaacaacga tcattatgat gggtgtggcg
    25021 attattgtcg tactcggcac tgagctggga tggtggtaac gtcacatcta aaaaatagca
    25081 aaggctgcct gtgtgcagcc tttgtgcaat ttacgcgtta acttttaatc ttcctgtaga
    25141 taaatagcac gacaatcgca ccaataacgg caaccacgaa gctgccaaaa ttgaagccat
    25201 cgactttacc aaagccaaac agcgtgctga tccaaccgcc gactacggca ccgactatcc
    25261 ccagcaggat agtcataaag aatccaccac catctttacc tggcatgatc cacttcgcca
    25321 gaataccggc aataagccca aaaataatcc atgacagaat gcccattgtt tcctcactta
    25381 tctgttttgc attagcgggt tagtcgctga taaaaagcat agcacaacat cgggagggca
    25441 agatttgtga cgagcatcac ggaggttttt ttgcgatggc gcagaaattg cgccatcaac
    25501 gatcagtgat aattaccaac cacaaacatc atgttcgttt tccgtgtcat aagaacgcac
    25561 ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg
    25621 cgtctctgtt tcataatcga cagaaacgcc attgctgtta ttggtgacgg tcacggtata
    25681 cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc
    25741 catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc
    25801 ttcaatgttg tgccactttt ctggagggaa caccgcaaac tgccccgctt cgataaggat
    25861 cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg
    25921 cataacggaa aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa
    25981 tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt
    26041 ctgtgggatt tgcagcatcc ttttccctcc ttcggtgaat gcgctgaaaa cggtttattc
    26101 cagccgtttc agggtacgcc tgataatttg ctttttaaat accatttatt ggttactttt
    26161 tagcaccata tcagagaaaa atcagggagg attatagatg ggaagcccgc gacaacgcgg
    26221 gctgaaagca tcaggattgc agcgtcgcca gtcgggcagc gaaacccacg aacatcaaac
    26281 caatcagtga gttgcccact ttagccagtt tctttttggt acgtatgtac tgcgtgacaa
    26341 aagcaccaga tataatcagg aagctcaaat agcagaaact caccagttcc agcgtcgtcg
    26401 ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac tgtacgaaaa
    26461 acgacacata gaacaaaatg gctttcggat tagtcaggct caaaattaac gcgcgtttaa
    26521 aaatagcacc gtattggggc tcatcggatt tggcctcgtt atttttaccc ttcagggtcg
    26581 cgtaaagaat tttactcccc agatagagca aataaaacgc accaagataa cgtacaatgt
    26641 tgaataatat cggggtggtc ttaattaatg tcgccattcc agcccatgcc agaaacatca
    26701 ataccgcatc gccaataaat acaccgcagg ccgcaagata accgcctttc ataccgctac
    26761 tgacgctatt tttgagtaca aacagggtat ttggccctgg caccaacaca ataaaaatgg
    26821 ccccaaccag ataggtccag taattcagaa ccccgtattc agcgaacaca ttaacctctt
    26881 taattatctt tcgatcatgc gcgattaaag gtgaatatgc taaccaatct gtagcggctt
    26941 agaaaggaga aaatcaggtt ttaacctgat atcaacccga taattgaatc attaatcagg
    27001 catgctccag tgaaaaattc gggtagtgct cggcaaaata ctggcgtaaa aattctaccg
    27061 ttatccgcac tttcgctgac gtcgccagcc ttgaaacata aacggcccag acgttcgctg
    27121 gctggtaata ttccggtagc acttgcacta aatgaccact ggcaatgttt tcgctaacat
    27181 cccaccagga acgcagagca atcccctgcc catcgagaca ccactgatgg acaatttctc
    27241 catgattaga cgacagtggg ccggtgactt taatcgcatg gtggccttct ttattgcgta
    27301 attgccagac accaaaagga tgatcgcgct ctttaatgac caggcacggc aatgcagaaa
    27361 gatcggttaa atgtttcggc gcgccatgtt gcgcaataaa ttccggcgac gcgcagagaa
    27421 tgcggtaatt ggtcgccagt ttgcgggcga ttaaattagg ggcgatgtca tcgccaatgc
    27481 gaatatcgag atcgacacct tcattgacca aatcgaccag ccggtcttcc acatcaaaac
    27541 gtaattccag ttgcggatac gccttcgcca gcgctgataa cgccggagcc accacctgtc
    27601 gtccaaaacc aaagctgctg ataatacgca gcatcccctg tggcacctga cgcacgtcag
    27661 aaagttcgtc catcatctga ccgacatcct gcaaaatccg ctgcgcccat tcataaatcc
    27721 gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg gtgtaacaac accacgttta
    27781 gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc cggtgaaacg cccagttctt
    27841 ccgccacggc ggcaaaacca gcccggcgag ccaccagcat aaagacgcgc aaatcattca
    27901 gcagcggtaa attattcatg attcgtgttt tatgtttcac cagttacggg gattaattcc
    27961 ttttcagtca attataggat ggtgatgttg tcaattttga tggtcaggaa gtgagaaccc
    28021 aatgatgaaa acgatgcgta ttgctgcgat cccgggagac gggattggca aagaagtcct
    28081 tcctgaaggg attcgcgtgt tacaggctgc cgctgagcgc tggggcttcg ccttgagttt
    28141 tgagcaaatg gagtgggcga gctgcgagta ttacagccat cacggtaaaa tgatgccgga
    28201 cgactggcat gagcaactta gccgtttcga cgccatctat tttggtgccg tcggctggcc
    28261 ggacaccgtt ccggaccata tttcgttgtg gggttcgctg ctgaaatttc gtcgtgaatt
    28321 cgaccagtac gtcaacctgc gcccggttcg tctctttcct ggcgttccct gcccgctggc
    28381 gggaaaacag cctggcgaca tcgattttta cgtggtcagg gaaaacaccg aaggcgaata
    28441 ttcctcgctc ggcggtagag tgaatgaagg tacagagcat gaagtcgtca ttcaggaatc
    28501 ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat gccttcgaac ttgcgcaaag
    28561 ccgcccacgt aagacgctaa cttctgccac taaatcaaac ggtttagcca tcagcatgcc
    28621 gtactgggat gagcgagtgg aagcaatggc cgagaattac ccggagatcc gctgggacaa
    28681 gcagcatatt gatattctct gcgcgcgttt tgtgatgcag ccggaacgct tcgatgtggt
    28741 ggtggcgtcc aatttgtttg gcgatatcct ttccgatctt ggcccggcct gcaccggcac
    28801 cattggcatt gccccatccg ccaacctgaa tccggaacgc actttcccct cgctcttcga
    28861 gcctgtccac ggttccgcgc cggatatcta cgggaaaaat attgctaacc ctatcgccac
    28921 aatttgggcc ggggcaatga tgctcgattt tctcggcaat ggcgatgagc gtttccagca
    28981 agcgcataac ggtattctgg cagcgattga agaagtgatt gctcacgggc cgaaaacacc
    29041 ggatatgaaa ggcagtgcca ccacgccaca ggttgccgac gcgatttgca aaattatttt
    29101 gcgttaaggt caaaccagtt tatttgaacc gcgtcactga cgcggttttg ttattcgttc
    29161 tttgcagtta ataacctgcg tcatttcacc ttttattgtt tccgtttcgt gttttatggc
    29221 tttccgtatt cttaattgtt taatttatgt aacatgcaaa taatcaattc gttaaacacg
    29281 acatacaact cattaccctg caaataatta caactggcaa aagccagaga cgggctttta
    29341 tgcccaggag atgactcatg atgagcaatg ttaagaaaaa agatgtgccg ctgataagca
    29401 tcagcctggt ggccattctt ttcatcgcag ctgcattaag ccttttccca caacaatcgg
    29461 ccgacgcggc caacgcaata tacacttttg ttacgcgtac gttaggttcc gcagtacagg
    29521 tattggtttt gctggcaatg ggactagtga tttatttagc caccagtaaa tacggcaata
    29581 ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc ctggctgttt atgtttattt
    29641 gtgccggttt aggttcttct acgctttatt gggggggtgc tgaatgggcc tattattatc
    29701 aaacgcctgg attaaatatc gcaccgcgtt cacaacaggc actcgaattt agcgtccctt
    29761 actccttctt ccactggggc atcagcgcct gggcaactta tacgctggcc tcattaatca
    29821 tggcttatca ctttcatgtg cggaaaaaca aaggtctgag tctttccggc attattgccg
    29881 cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg atgttcctga
    29941 tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc tttactcgtg
    30001 gactttccgc actgaccggt ttacccgata acttcaccgt gcaggcattt gtgatcctgc
    30061 tttccggcgg cattttttgc ctaagctcat ggattggtat caacaacggt ttgcaacgtc
    30121 tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg attgtcggtc
    30181 caaccgaatt tattaccaac agcatcatca atgccatcgg cctgaccacg caaaacttcc
    30241 tgcaaatgag cttattcacc gatccgcttg gcgatggttc atttacccgc aactggaccg
    30301 ttttctactg gctgtggtgg atctcataca cccctggcgt agcaatgttt gtcacccgcg
    30361 tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc agcaccgtcg
    30421 gttgctggtt cttcttcggc gtaatggaaa gctatgccat tcatcagttt atcaatggcg
    30481 taatcaacgt cccacaggtg ctggaaactc tgggcggcga aacagctgta cagcaagttc
    30541 tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggcgtg atgattattt
    30601 tccttgcctc gcatatggat gcagtggcct acaccatggc ggcgaccagt acgcgtaatc
    30661 tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc
    30721 tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa accaccgtcg
    30781 tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga tttattcgct
    30841 ggctgaaaca ggattacgcc gacattccgg ctcatcaagt tgaacattat ctcccgcaga
    30901 caccggttga agccctggaa aaaacaccag tgctccctgc gggaaccgta ttcaaaggcg
    30961 acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct gagccctgac
    31021 tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg cgtggaccat tcctgcccgt
    31081 ttttataccg atcagaacgc ctttgaacac gaaaaagaga acgtcttcgc caaaagctgg
    31141 atttgcgtcg ctcacagcag cgaactggcg aatgccaatg attatgtgac gcgtgagatc
    31201 attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc gttctataac
    31261 gtgtgtccgc accgtggtca tcagttgttg agcggtgaag gaaaagcaaa aaatgtgatt
    31321 acctgcccat atcacgcatg ggcattcaaa ctcgatggca atctggccca tgcacgtaac
    31381 tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt gcgtctggaa
    31441 gaatatgccg gattcgtctt catcaacatg gaccccaacg ccaccagcgt agaagatcag
    31501 ttacccggtc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga tctgaaactg
    31561 gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga taactatctc
    31621 gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca ggttgatcgt
    31681 tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa accgtccgaa
    31741 cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg gctgtggccg
    31801 tgcacgatgc tgaatgtcac cccgatcaaa gggatgatga cggtcattta tgaattcccg
    31861 gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga agagttaacc
    31921 gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga agatttacgt
    31981 ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc
    32041 atggccgaca gtagcggtag cggcatttcc gaacatggta tcgcccattt ccataatctg
    32101 ctggcgcagg tatttaagga ctaatgacat cggcggcggt attttccgcc gccgggctga
    32161 tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg tgagtcaggt
    32221 ggaaccgctt accgaacagg tgaaacgctt cacgccggtg gcaaccgatg gcaaaccgct
    32281 gcctgcgttc accggaggaa gtcacatcat agtgcagatg agcgatggtg ataaccagta
    32341 cagtaatgcg tattcactac tgagttcacc gcataacacc tctagttatc agattgccgt
    32401 tcgactggaa gaaaactcgc gcggcggttc ccgctttttg catcagcagg taaaagtggg
    32461 cgatcggtta acgatctcaa cgcctaataa cctgtttgcg ctaattccct cagccagaaa
    32521 gcatctgttt atcgcgggcg gtattggtat cacccctttc ctgtcgcaca tggcagagtt
    32581 gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag aaagttgcgc
    32641 atttcgtgat gagctagtcc agcatccgca ggctgagaaa gtccatttgc atcattcatc
    32701 aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg gcacacacgt
    32761 ttatacctgt ggccccgagg cgctaattga agcggtaaga agtgaagctg cgcgtctgga
    32821 catcgctgcc gatacgctgc actttgagca atttgctatc gaagacaaaa ccggcgatgc
    32881 atttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag agatgactat
    32941 tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt
    33001 atgcggaacc tgcgaaacgg caatactgga aggtgaagct gaccatcggg atcagtattt
    33061 tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg
    33121 taaacgcctg gtgttggatt tgtagtttgc cgaagccgga tgtggcgctg agcgcgccca
    33181 gtccggcttc ggaaaatttt actgcggata ttcctgcaat aaattgtgta atgcttccgc
    33241 catcagctca ccacgccagc cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt
    33301 ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt cggcgctgat
    33361 cttgtgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaacg ctttacgata
    33421 acccggcatg tccatcaggt taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg
    33481 cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc taccggataa
    33541 acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgctaccg accacaaatg
    33601 ctcttcacgc acgacaaagt tcaccgccag atcgcgctct cgcgccttgc gcagtcgcca
    33661 gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aattgccagg cattggtgat
    33721 atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca tcaggcgaca
    33781 ttcatccagc gccgctggta gccagccgga ggcctccgtt tctaccataa gcttggcggt
    33841 gatcggtaac agataccaga catccgccgc tgcatattca cactgacgtt cggtcagcgg
    33901 tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg aatactcttc
    33961 caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt
    34021 gtcaatcagg ggttgtggta attcgccaaa gacattgagg aacacttcca gatcttcact
    34081 gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca gcggtgacca
    34141 gtcggtgatc ccgagaggat caattagcgc cagatgctcg ccatcgaaaa gttgaatcaa
    34201 ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatccaggg ctatcgccgg
    34261 aaaggcacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa tcatttggta
    34321 attcaaattg ttttctcttt agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga
    34381 cgactgactt aacgctcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
    34441 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
    34501 cggtactttg tagcccgtga gctgacggcg gcaaaaggtc accagtgact cttcggtaag
    34561 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
    34621 accaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
    34681 gggatagacg ttaaaaccgg aaaccaaaat catgtctttt ttacgatcga caatgcgcag
    34741 gaatccttct tcatccatta ccgcgatgtc gccagtgtgt aaccagccgt ttttgatgat
    34801 ttcatcggta gcatccggac gctgccaata gcccagcatc acctgtggac ctctgacgca
    34861 aagttcacct ggctgacctg gcgatacttc attatcgtcg tcatccacca gtttggcttc
    34921 cgtcgacggc accggcaaac caatgctacc gctatgataa tcaatatcat aggggttcac
    34981 gctgaccagc ggcgcacatt cggtcaggcc ataaccttcc agcagatact gtccggtcag
    35041 tttcacccag cgttctgcca ccacttgctg aacaggcatc ccaccgcctg cagaaaggtg
    35101 cagactggag aaatccagtt gttgaaactc tttattgttc agcaacgcgt tgaacaaggt
    35161 gtttacgccg gtgatagcgg taaacggata tttcgccagc tctttgacca gccccggaat
    35221 atcccgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca gcaggcagtt
    35281 aatggtcagt gcaaaaatgt gatacagcgg cagcgccgtc accaccagct ctttgcccgg
    35341 atgcagcaga ggcccatacg tagcgttaac ttgttccagg ttcgccagca tattgcggtg
    35401 agtcagcatc gcgcctttcg ccacacctgt ggtgccgcca gtgtattgca ggaaggctaa
    35461 atcttctggc accagttcag gtttgacata ctgcatacgg tagccgttgt gcagggcgct
    35521 acgaaatgaa atggcatctg gcagatgata tttcggcacc agacgtttga tgtatttaac
    35581 aacgaagtta accagtgtgc ctttcgctgt ggaaagctga tcgcccatac gggtcaggat
    35641 aacgtgctga actgcggttt tatcgaccac tttttccagc gtgtgagcaa agttggacac
    35701 aatgacaatc gccgatgcgc cgctatcgtt aagctgatgc tcaagctcac gcggggtata
    35761 caacgggtta acgtttacga cgatcatccc ggcacgcaaa atgccaaaca gcgccaccgg
    35821 atattgcaat aaattaggca tcatcaacgc aacgcgatcg cctttcttca gccccaaccc
    35881 ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc agcttgcgga aggtcatcac
    35941 ctcccccata ttcacaaacg caggctgatc ggcgtagcgc gcgaccgact gctcaaacat
    36001 atctaccaga gattgataac ggtcagggtt gatctccgtc ggaacgtccg cgggataacg
    36061 gttaagccaa accttcttca attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac
    36121 cccaaatgat atacatgccg ttaacataat attaactcat cataccagct tgataattac
    36181 ccaacgaaaa ggttgcgaag cgcgtcacta tttattttta tctttaccgt aagaatgcag
    36241 aaacagcgga ccagccgctg tttctttttc tttaaaaaca agcaattatt cagttacaac
    36301 tgtttgtact ctcgcggggc cgggattata ccagccccat ccgccgtggc catagggcca
    36361 gccacgaccg ccataaaacc agggatcaat cggctgaggc ggcataatca cctgctgggt
    36421 taaatgccaa cgtttgtaac ctgttgcctg catcaccata aatttatagg gcgtgttgcc
    36481 gatttttccg tcaaccgcac cagtgattgg cccgactacc gtaaccagtt gtccacggaa
    36541 atccaccggg tccagaaaac cgttcacatc ggcataaatg cgaccgcgag aaggttctcc
    36601 cagcgtcggt ctggctccgc tgtccagcgg tacggtagca atttccaggc gggttttccc
    36661 ttgctggttt tgtaccgcaa ccactttgcc accaaagcgt gcctcctgac caacgtacag
    36721 ctgcggcgca ctcatcaccc gaactaaatc ttgttgcggc gtgggactgc tgcctttaat
    36781 ggcgtccggc acagtgacac aaccgctcag cattagcgca aacgtacctg ccagtatgcc
    36841 tttgataaca ttcttttgaa ccgccatgat gcgactcctt tttttctcag ggcatactct
    36901 taagattcat tctttgcccg gaagtttctt ccatgcgacg ttgttacgta aataaaccgg
    36961 ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac atctgacaag caatcggcag
    37021 catatcttct gcagcgggca gtaaaacttc tccgtcgcgc aaaaccagtc cgctctcttt
    37081 accgagatcc ggccaggctt gccagcctgt acctaccgtc acccattccc cggaaagctg
    37141 ttgcattcgt tcatgaacga gttcgggttt gagtacggct tcggtttctt caccgtgcca
    37201 gataccgttt tcatcacgct gatattcggc ccagtaaact tcgcccattc gcgcgtcaat
    37261 ggctgacagc acgcgtgttg cgccgttttt gcgccacgcc ccttgcgcca tcgtcattag
    37321 cgtggagacg ccaatcatcg gtaattccgc gccaagcgcc agcccttgcg cgatgccaat
    37381 accaatgcgc acgcctgtaa agctaccggg gccgcgcccg taagccagag cgttaatatc
    37441 agtcagggaa gttccgctgg tggtcaggat atcctgcacc atcggtaaga ttcgttgagt
    37501 atgttcacga gggcaaagct caaaatgagc gttgacagta ccgtcgttcc acagggcgac
    37561 agagcaggcc tctgtcgcgg tatcgatagc cagaattcgc atgggtcttc gtgcttagat
    37621 caataaaaag gcgcgcatca taccatactc cgtaacaaat tacctggagg atggtatcgc
    37681 aaggaaacga accgcacggg caatgtcacg ggtgcgtggc gcgggcggca gactggcgag
    37741 aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca caaattacca aaaccccgcg
    37801 atcgtcggcg tcgcgaatca gtcgcccgac tccctgcttg agagtaatca ccgcatccgg
    37861 cagttgtact tcatcgaacg ggtcgccacc gcgcaaacgg caatcttcca tgcgcgcttt
    37921 taacaatgga tcatcaggcg aggtaaacgg caatttgtcg ataatcacca atgacaatgt
    37981 atcgccacgc acgtccaccc cctcccagaa actactggtt gccacaagaa gcgcgttacc
    38041 ggcactgaca aattgctgca acagttgccc tttgctggtt tccccctgca ataatacggg
    38101 aagcgtcatg gtagcgcgga actgctcggc cagatcgcgc atcatggcgt gcgaggtaca
    38161 aagcataaaa cagcgaccgt tgttagcttc gatgatcggg cgcagcatag ccgccagttg
    38221 ccgcgcggaa cccggttgat tggtttgcgg caggttgcgc ggcacacaga gtaacgcctg
    38281 gcggctgtaa tcaaacgggc ttggcaatag caacgactcc gcctgttcta tcccaagccg
    38341 cgaggtgaaa tgatgcagat cgtcgtttac cgacagcgtt gctgaggtga agatccagct
    38401 accgggcttt tgcgccatta actctttgaa tttatccgcc acgctgagcg gcgtgagagc
    38461 cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg tagcccggct gattgatctc
    38521 ttttagccgc ttcagccgtg tgcgatacaa cgtggcgcgc tcaaatgccg catccagcaa
    38581 ggcggaacgc cccagtgaca gtttcgccac gtcataacaa agttccaggg tgtcatcgag
    38641 cagtaaaaat gcccgctgaa tttgcggatt agctaacagc tcacgcaggt taccgcgata
    38701 acctggttca ccgagttgca gacgaaaatc ctgcgcactc tgggcaagac gatcagcgca
    38761 cttttgtaac tgctgggtgt cttttaattc ggtgcggtag gcgatggtga tgtcttttgc
    38821 caggtcgagc agttgtcgac tggagagtga ctgaccaaaa tactggctgg caatgtccgg
    38881 tagctggtgg gcttcgtcga agatcatgac gtccgcttcc gggatcaact cgccaaatcc
    38941 actctcttta accaccatat ccgccagaaa gagatgatgg tttaccacca ccacatcggc
    39001 gtccatcgct tttttacgtg ctttgaccac aaagcaatct ttatacatcg ggcagtcgct
    39061 gccaagacag ttgtcgttgg tgctggtgac cagcggccac gcctgcgaat cttccgccac
    39121 gctgacgcag gtgctgatat caccatcgac tgtttgatta gaccaggagc gcagcaggat
    39181 cacatcgctt aatatttgta ccggaagatc gccccccgcc agcgcctgct gttcgagacg
    39241 ttcgaggcag aggtagtttg agcgcccttt cagcaacgcc acgttgcccg tgtatttcaa
    39301 cgcctttgag accgtcggca aatcgcggct atagagctga tcctgcaacg cttttgagcc
    39361 ggtcgagata atgactttct ttttcgcccg cagcgcaggg gccaggtaag cgtaggtttt
    39421 gcccgtaccg gttcctgctt ccaccaccag cggctggcct ttttctatcg cctgggtgac
    39481 ggctaccgcc atctgtcgct gtggttctcg cggcttaaag cctggtatcg ctttcgccag
    39541 ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct gttgatttga acagggatta
    39601 tgtcaggatg agggcgcttt cgccagttga agtggtgacg gcgacctcac attgtggcag
    39661 tctttgcagc acgaaaatgg aaggataatg aggataaaat gatgactatc gttcgtatcg
    39721 atgctgaagc ccgctggtct gatgtagtaa tccacaacaa cacgctctac tacactggtg
    39781 taccggaaaa cctcgacgcc gatgcctttg agcaaaccgc caacaccctg gcgcagattg
    39841 acgccgtgct ggaaaaacag ggcagcaata aatcgagcat tctggatgcc accattttcc
    39901 tggccgataa aaacgacttc gcggcgatga ataaagcgtg ggatgcctgg gttgtcgcgg
    39961 gtcatgcgcc ggtgcgctgc acggtacaag cgggtttgat gaacccgaag tataaagttg
    40021 aaattaagat tgtggctgcg gtgtaagctt tatcgaagca aaataagtca gacgataatt
    40081 tatcgataat actggtcggt tatacataaa tcgaccagag aataagatat tactgttgat
    40141 gcttctgcta attcattatt tatttttata atttcaattt tatctataaa actaactata
    40201 taaataaaca taaccatgcc gttgtgttcg ccctttgatt agcaactctg gttttctttc
    40261 ttagctgaac aatctggtta tagcgtgcgg actgaccgtt aattttctcc atcttactgg
    40321 ataacacacc atgagctgct acctttaaaa cacctgaata tcaacaggtt aactcgtgcc
    40381 atatcgttat aatccctttg atattagatg caaattaagg tcatatatag ccttatcgat
    40441 aacatggtta attttaagga taagaatatg cctgctgtaa tagataaagc cctggatttc
    40501 attggtgcca tggatgtatc agcgccaaca ccaagttcga tgaatgaaag cacggcgaag
    40561 ggaatcttta aatatttaaa agaactggga gtacccgcga gtgccgctga tattacggcg
    40621 cgagctgacc aggaaggctg gaaccctggg ttcacggaaa aaatggttgg atgggcaaaa
    40681 aaaatggaga caggtgaacg ctctgtgatt aaaaatcctg agtacttttc aacatatatg
    40741 caggaagaac taaaagcact ggtctgagtt aaatttatat cagcataaat gggtcaggac
    40801 gcttttaatc acatataaaa agcgtccttt ttctcacaat caaataatca tcagcttcta
    40861 taacgaaatc gatacggatt agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt
    40921 cgctcgccgg agtggttggc acgcagctct tctgccacca gcgcgattgc ctggccgctg
    40981 ctcatccctt gtgccatcag ttcctggatc cgctcgacag ctttttgctg ctgttcatgg
    41041 gtgagtgaag gtaaacctgc aaacattgtt aactcctgct aaattgttgg cgctaattat
    41101 ttcatgctac ccggcacata gccagtagag tcaggactga tgaagacgtt atctcccgct
    41161 gtgattactt taccctggcg tcaggacgcc gctgaatttt atttctcccg cttaagccac
    41221 ctgccgtggg cgatgctttt acactccggc tatgccgatc atccgtatag ccgctttgat
    41281 attgtggtcg ccgatccgat ttgcacttta accactttag gtaaagaaac cgttgttagt
    41341 gaaagcgaaa aacgcacaac gaccactgat gacccgctac aggtgctcca gcaggtgctg
    41401 gatcgcgcag acattcgccc aacgcataac gaagatttgc catttcaggg cggcgcactg
    41461 gggttgtttg gctacgatct gggccgccgt tttgagtcac tgccagaaat tgcggaacaa
    41521 gatatcgttc tgccggatat ggcagtgggt atctacgatt gggcgctcat tgtcgaccac
    41581 cagcgtcata cagtttcttt gctgagtcat aatgatgtca atgcccgtcg ggcctggctg
    41641 gaaagccagc aattctcacc gcaggaagat ttcacgctca cttccgactg gcaatccaat
    41701 atgacccgcg agcagtacgg cgaaaaattt cgccgggtac aggaatatct gcacagcggt
    41761 gattgctatc aggtgaatct cgcccagcgt tttcatgcga cctattctgg cgatgaatgg
    41821 caggcattcc ttcagcttaa tcaggccaac cgcgcgccat ttagcgcttt tttacgtctt
    41881 gaacagggtg caattttaag cctttcgcca gagcggttta ttctttgtga taatagtgaa
    41941 atccagaccc gcccgattaa aggcacgcta ccacgcctgc ccgctcctca ggaagatagc
    42001 aaacaagcag aaaaactggc gaactcagcg aaagatcgtg ccgaaaatct gatgattgtc
    42061 gatttaatgc gtaatgatat cggtcgtgtt gccgtagcag gttcggtaaa agtaccagag
    42121 ctgttcgtgg tggaaccctt ccctgccgtg catcatctgg tcagcaccat aacggcgcaa
    42181 ctaccagaac agttacacgc cagcgatctg ctgcgcgcag cttttcctgg tggctcaata
    42241 accggggctc cgaaagtacg ggctatggaa attatcgacg aactggaacc gcagcgacgt
    42301 aatgcctggt gcggcagcat tggctatttg agcttttgcg gcaacatgga taccagcatt
    42361 actatccgca cgctgactgc cattaacgga caaatatact gctctgcggg aggtggaatt
    42421 gtcgccgata gccaggaaga agcggaatat caggaaactt ttgataaagt taataagata
    42481 ttacgccaac tggagaagta agacgtggaa taccgtagcc tgacgcttga tgatttttta
    42541 tcgcgctttc aacttttgcg cccgcaaatt aaccgggaaa ccctaaatca tcgtcaggct
    42601 gccgtgttaa tccccatcgt ccgtcgaccg caaccggggt tgttgctgac tcagcgttca
    42661 attcatctgc gtaaacacgc tggacaagtg gcattccctg gaggtgcagt cgatgacacg
    42721 gacgcatcag ttatcgccgc cgcgctgcgc gaagctgaag aagaggttgc tataccgcct
    42781 tccgccgttg aagttatcgg cgtgctgccg cccgtcgata gcgtaactgg ctaccaggta
    42841 accccagtgg tcggcattat cccgcccgat ctgccgtatc gcgccagtga agatgaagtt
    42901 tccgctgtgt ttgaaatgcc gctcgcccag gcattacatc tgggtcgtta tcacccttta
    42961 gatatctacc gccgcggcga ttcacatcgg gtatggctat cctggtacga acagtatttt
    43021 gtatggggaa tgaccgcagg cataattcgt gagctggcgc tgcaaattgg tgtgaaaccc
    43081 tgactatact tatctttaca tctacaaaac actacttgag acaatcatcg caatattagt
    43141 taaatcgcgg tttttgatta gtttaattca tgtgaatagt taagccagtc gccgcgttcc
    43201 ctcttacact atgcgctgtt attagttcgt tactggaagt ccagtcacct tgtcaggagt
    43261 attatcgtga ttagtctatt cgacatgttt aaggtgggga ttggtccctc atcttcccat
    43321 accgtagggc ctatgaaggc gggtaaacag ttcgtcgatg atctggtcga aaaaggctta
    43381 ctggatagcg ttactcgcgt tgccgtggac gtttatggtt cactgtcgct gacgggtaaa
    43441 ggccaccaca ccgatatcgc cattattatg ggtcttgcag gtaacgaacc tgccaccgtg
    43501 gatatcgaca gtattcccgg ttttattcgc gacgtagaag agcgcgaacg tctgctgctg
    43561 gcacagggac ggcatgaagt ggatttcccg cgcgacaacg ggatgcgttt tcataacggc
    43621 aacctgccgc tgcatgaaaa cggtatgcaa atccacgcct ataacggcga tgaagtcgtc
    43681 tacagcaaaa cttattattc catcggcggc ggttttatcg tcgatgaaga acactttggt
    43741 caggatactg ccaacgaagt gagcgtgccg tatccgttca aatctgccac cgaactgctc
    43801 gcgtactgta atgaaaccgg ctattcgctg tctggtctcg ctatgcagaa cgaactggcg
    43861 ctgcacagca agaaagagat cgacgagtat ttcgcgcatg tctggcaaac catgcaggca
    43921 tgtatcgatc gcgggatgaa caccgaaggt gtactgccag gcccgctgcg cgtgccacgt
    43981 cgtgcgtctg ccctgcgccg gatgctggtt tccagcgata aactgtctaa cgatccgatg
    44041 aatgtcattg actgggtaaa catgtttgcg ctggcagtta acgaagaaaa cgccgccggt
    44101 ggtcgtgtgg taactgcgcc aaccaacggt gcctgcggta tcgttccggc agtgctggct
    44161 tactatgacc actttattga atcggtcagc ccggacatct atacccgtta ctttatggca
    44221 gcgggcgcga ttggtgcatt gtataaaatg aacgcctcta tttccggtgc ggaagttggt
    44281 tgccagggcg aagtgggtgt tgcctgttca atggctgctg cgggtcttgc agaactgctg
    44341 ggcggtagcc cggaacaggt ttgcgtggcg gcggaaattg gcatggaaca caaccttggt
    44401 ttaacctgcg acccggttgc agggcaggtt caggtgccgt gcattgagcg taatgccatt
    44461 gcctctgtga aggcgattaa cgccgcacgg atggctctgc gccgcaccag tgcaccgcgc
    44521 gtctcgctgg ataaggtcat cgaaacgatg tacgaaaccg gtaaggacat gaacgccaaa
    44581 taccgcgaaa cctcacgcgg tggtctggca atcaaagtcc agtgtgacta atacttctta
    44641 ctcgcccatc tgcaacggat gggcgaattt atccccgctt tctcgtctgc tgtaatattc
    44701 cccactacac ttccactgtt gcgtcaggcg tttgtcgcca tacgcttaca gagtggcccg
    44761 catgcaaaaa gcacaacgga tcattaaaac ctatcgccgt aatcgaatga ttgtttgtac
    44821 gatttgcgca ctcgttacgc tcgcttcgac cctgagcgtg cgatttattt cacagcgtaa
    44881 cttaaatcaa caacgggtag tacaattcgc caatcacgct gtagaggaat tagataaagt
    44941 actgcttccc ctacaggcag gtagcgaagt cttgcttccg ctgattggtc tgccctgctc
    45001 tgtcgcccat ttgccattac gtaaacaggc ggcaaaactc caaactgtgc gatccattgg
    45061 cctggtgcaa gacggcacac tttattgctc cagcattttt ggttatcgca atgtgcccgt
    45121 cgtggacatt ctggctgaac ttcctgcacc gcaaccactt ttacgcctga cgaccgaccg
    45181 tgtcctgatt aaaggcagtc cggttttgat tcaatggacg cctgcagcga gcagtagcaa
    45241 tgctggggtc atggagatga ttaacatcga cttactgacg gcaatgctgc ttgagccaca
    45301 actgccgcaa atcagtagcg ccagcctgac ggtggacaaa cggcatttgc tctatggtaa
    45361 tgggctggta gattcccttc cacaacctga agacaatgaa aactatcagg tttcttcgca
    45421 acgcttttct tttaccatta acgttaatgg tccgggggct ccggcgctgg catggcacta
    45481 tcttccaaca caattaccgc tggcggtact gctaagttta ctggtgggct acatcgcctg
    45541 gctggcgacc gcttaccgaa tgagcttttc ccgcgaaatc aatctgggcc tggcgcaaca
    45601 tgagttcgaa ttgttctgtc agcctttgct taatgcgcgc agccagcaat gtattggtgt
    45661 agagattttg ctgcgctgga acaatccgcg tcagggctgg atttcaccgg atgtgtttat
    45721 tcctatcgcg gaagaacatc atttaattgt gccactgacc cgctatgtga tggcagaaac
    45781 cattcgtcag cgccatgttt tcccgatgag tagtcagttt catgttggca ttaacgtcgc
    45841 acccagccat tttcgccgtg gtgtgctgat aaaagatctc aatcagtact ggtttagcgc
    45901 tcacccgatt cagcaactga tcctcgaaat caccgaacgc gatgccttac tggatgttga
    45961 ttatcggatt gcccgcgagc tacatcgtaa aaacgtcaaa ctggcgattg atgacttcgg
    46021 caccggcaac agttcatttt cctggcttga aacattacgt cctgacgtgc tgaaaattga
    46081 taagtcattt accgcagcta taggttctga cgcggttaac tcgacggtga ccgatatcat
    46141 catcgcgctg gggcaaagac tgaatattga actggtggcg gagggcgtgg aaacgcaaga
    46201 acaggcgaag tatttgcgcc gtcatggcgt gcatattttg caagggtatt tgtacgcaca
    46261 gccgatgccg ctacgtgatt ttcccaaatg gctggcgggc agccaaccgc cgcccgcccg
    46321 gcataatgga catatcacgc ccattatgcc gttacgttaa cgcttactca tcttcatcgt
    46381 gcgccggttg ctctttaaca atgcgaacca gatcaacacg ataatcattg gcttcaatga
    46441 tggtgatatg cagtggccct acatcaatca catcgcccac acggggaatg tgaccatttg
    46501 ccgagatcac gaggcccgcg accgtcgcga tatcatcgtc atcggcaagg tgctcaacat
    46561 caagcgcctg ctgcaaggca tgcaaatctg taccgccttt taccagccag ccgtcaccat
    46621 cagtaatgat ttccggcgtt tcgtcagcgt ccgggaattc acccgcaatg gcttccagca
    46681 catccagcgg cgtgaccaag ccttgtacca caccaaactc gttggtcacg ataacaaagc
    46741 tcccgcgagc acgacgcagc acgcccagca ggttgatcgg atcgagggtt tcagggacga
    46801 taatcgccgg agacgccgaa gcaatcgccg ccacatcaac gccctcttcc agcgccacca
    46861 gcagttcttt agcacgtacg attccgatga tttcatccag ttcaccgcga cataccggga
    46921 acaggctgtg cggtgaggag agcagctgct cgcggatttc atcgaccccg agattagcgt
    46981 caacccagct tatttcaccg cgcggcgtca tgatcccgcg cagagaacgc gacgccagcg
    47041 tcagtacgcc gttaatcatg taacgttctt cttcggcaaa tgcaccttcc gggatcggca
    47101 tcggcatcgg gttatcggca tcgtgctgaa cattggcctg acgtttcccg cccatcaaac
    47161 gcaggatggc atcggcagta cgcgctcgca gcggcaaagt cgactggtga cgaataaagt
    47221 tgcgacgcgc aatctggtta aacacttcga tgatgatcga gaagccaatc gcggcataca
    47281 ggtaaccttt cggaatgtgg aaaccgaaac cttctgccac cagactcaga ccaatcatta
    47341 acaggaagct cagacagagc accaccaccg tggggtgctg gttaacgaat cgcgtcagcg
    47401 gtttggatgc cagcaacata accgccatcg caatcactac cgccgccatc atcaccggca
    47461 gatggttaac catccctact gcagtaatta ccgcatccaa cgagaagacg gcgtcaagga
    47521 tgacaatctg tgtgacgacc acccagaaac tggcgtagcc tttaccgtgg ccggaatcat
    47581 gatcgcggtt ttccagccgt tcatgcagtt cggttgttgc tttgaacagc aagaatatcc
    47641 ccccgaacaa cataatcagg tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg
    47701 gtttggtcag cgtgaccatc catgaaatca gcgacagcag ccccagacgc ataatcagcg
    47761 ccagtgataa ccccagcaaa cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa
    47821 gaatggcgat gaagaccagg ttatcgatac ccagcacaat ttcgagaaca acaagcgtga
    47881 gtagccccgc ccaaattgag gggtccatta agaattccat gacaagctcc tgcttaagga
    47941 atagctattc gacgccagaa ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg
    48001 gcatagagtg ccagaaaggc aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg
    48061 atccatactg cgggctactg ccctatactc catgattgtt aaacgggagt taaacatatc
    48121 agagacgcct ctgatttggc aaagatttac cttcctttgc aaacgaatgt gacaaggata
    48181 ttttaccttt cgaaatttct gctaatcgaa agttaaatta cggatcttca tcacataaaa
    48241 taattttttc gatatctaaa ataaatcgcg aaacgcaggg gtttttggtt gtagccctta
    48301 tctgaatcga ttcgattgtg gacgacgatt caaaaataca tctggcacgt tgaggtgtta
    48361 acgataataa aggaggtagc aagtgaccat tgctattgtt ataggcacac atggttgggc
    48421 tgcagagcag ttgcttaaaa cggcagaaat gctgttaggc gagcaggaaa acgtcggctg
    48481 gatcgatttc gttccaggtg aaaatgccga aacgctgatt gaaaagtaca acgctcagtt
    48541 ggcaaaactc gacaccacta aaggcgtgct gtttctcgtt gatacatggg gaggcagccc
    48601 gttcaatgct gccagccgca ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt
    48661 taacattcca atgctcgtgg aaacgttaat ggctcgtgat gatgacccaa gctttgatga
    48721 actggttgcg ctggcagtag aaacaggccg tgaaggcgtg aaagcactga aagccaaacc
    48781 ggttgaaaaa gccgcgccag cacccgctgc cgcagcaccc aaagcggctc caactccggc
    48841 aaaaccaatg gggccaaacg actacatggt tattggcctt gcgcgtatcg acgaccgtct
    48901 gattcacggt caggtcgcca cccgctggac caaagaaacc aatgtctccc gtattattgt
    48961 tgttagtgat gaagtggctg cggatactgt tcgtaagaca ctgctcaccc aggttgcacc
    49021 tccgggcgta acagcacacg tagttgatgt tgccaaaatg attcgcgtct acaacaaccc
    49081 gaaatatgct ggcgaacgtg taatgctgtt atttaccaac ccaacagatg tagagcgtct
    49141 cgttgaaggc ggcgtgaaaa tcacctctgt taacgtcggt ggtatggcat tccgtcaggg
    49201 taaaacccaa gtgaataacg cggtttcggt tgatgaaaaa gatatcgagg cgttcaaaaa
    49261 actgaatgca cgcggtattg agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa
    49321 aatgatggat ctgatcagca aaatcgataa gtaacgtatt gtgttgatta tcagtcagtt
    49381 ttcacacttt agtcatacgt aaacaggaga agtacaatgg agattaccac tcttcaaatt
    49441 gtgctggtat ttatcgtagc ctgtatcgca ggtatgggat caatcctcga tgaatttcag
    49501 tttcaccgtc cgctaatcgc gtgtaccctg gtgggtatcg ttcttgggga tatgaaaacc
    49561 ggtattatta tcggtggtac gctggaaatg atcgcgctgg gctggatgaa catcggtgct
    49621 gcagttgcgc ctgacgccgc tctggcatct atcatttcta ccattctggt tatcgcaggt
    49681 catcagagca ttggtgcagg tattgcactg gcaatccctc tggccgctgc gggccaggta
    49741 ctgaccatca tcgttcgtac tattaccgtt gctttccagc acgctgcgga taaggctgct
    49801 gataacggca acctgacagc gatttcctgg atccacgttt cttctctgtt cctgcaagca
    49861 atgcgtgtgg ctattccggc cgtcatcgtt gcgctgtctg taggtaccag cgaagtacag
    49921 aacatgctga atgcgattcc ggaagtggtg accaatggtc tgaatatcgc cggtggcatg
    49981 atcgtggtgg ttggttatgc gatggttatc aacatgatgc gtgctggcta cctgatgccg
    50041 ttcttctacc tcggcttcgt aaccgcagca ttcaccaact ttaacctggt tgctctgggt
    50101 gtgattggta ctgttatggc agtgctctac atccaactta gcccgaaata caaccgcgta
    50161 gccggtgcgc ctgctcaggc agctggtaac aacgatctcg ataacgaact ggactaacag
    50221 gtgagcgaaa tggttgatac aactcaaact accaccgaga aaaaactcac tcaaagtgat
    50281 attcgtggcg tcttcctgcg ttctaacctc ttccagggtt catggaactt cgaacgtatg
    50341 caggcactgg gtttctgctt ctctatggta ccggcaattc gtcgcctcta ccctgagaac
    50401 aacgaagctc gtaaacaagc tattcgccgt cacctggagt tctttaacac ccagccgttc
    50461 gtggctgcgc cgattctcgg cgtaaccctg gcgctggaag aacagcgtgc taatggcgca
    50521 gagatcgacg acggtgctat caacggtatc aaagtcggtt tgatggggcc gctggctggt
    50581 gtaggcgacc cgatcttctg gggaaccgta cgtccggtat ttgcagcact gggtgccggt
    50641 atcgcgatga gcggcagcct gttaggtccg ctgctgttct tcatcctgtt taacctggtg
    50701 cgtctggcaa cccgttacta cggcgtagcg tatggttact ccaaaggtat cgatatcgtt
    50761 aaagatatgg gtggtggctt cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg
    50821 tttgtcatgg gggcattggt taacaagtgg acacatgtca acatcccgct ggttgtctct
    50881 cgcattactg accagacggg caaagaacac gttactactg tccagactat tctggaccag
    50941 ttaatgccag gcctggtacc actgctgctg acctttgctt gtatgtggct actgcgcaaa
    51001 aaagttaacc cgctgtggat catcgttggc ttcttcgtca tcggtatcgc tggttacgct
    51061 tgcggcctgc tgggactgta agactgttgt acactaccgg ggccttttgg ccccgttttt
    51121 ttatctggag gattaatgac aatcacggac ctggtactga ttcttttcat cgccgcactc
    51181 ctggccttcg cgatctacga tcagttcatc atgccccgcc gtaacggccc caccctgctg
    51241 gcaattcctt tgctccggcg tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc
    51301 attcttatct ataacaacgt cacgaatcat ggtgcgttaa taacgacatg gttattaagc
    51361 gcactggctc tgatgggttt ttatatattc tggatccgcg ttccgaagat catctttaaa
    51421 caaaaaggtt ttttcttcgc caatgtctgg attgaatata gccgaatcaa agcgatgaac
    51481 ttgtcggaag atggcgtgtt ggtgatgcaa ttagaacagc gtcggctgtt aatccgcgtt
    51541 cgaaatatcg acgatctgga aagaatttat aaacttctcg tttcaactca atgagttatt
    51601 aatttagcca aagctatgtt tagtgtattt ttaataatca gacatagctt aggctatatt
    51661 acctcttccc ttatttgtta tttattataa cgtttcattg atatataaat ctaaatgaaa
    51721 atcgttatca acaaagcaat aaaataatat attccaacag ttgttttata ttctcaaaat
    51781 atgttaaggt tgcgccctca tttggggagt agccaatttc cagattccgg aaatgtacgt
    51841 gtcaacatac tcgttgcaaa acgtggcacg tacggactga agactttcag tcaggcgaga
    51901 ccatatgcac atcaatcgct atgcctgcat gaacgcgaaa acccatcgcg gcttgttgtt
    51961 catgtatgca tgtttgctgg gggcgatgat gtgttttatg gataccccgg tcaggacgtt
    52021 gtcatgaata tcactgctac tgttcttctt gcgtttggta tgtcgatgga tgcatttgct
    52081 gcatcaatcg gtaaaggtgc caccctccat aaaccgaaat tttctgaagc attgcgaacc
    52141 ggccttattt ttggtgccgt cgaaaccctg acgccgctga tcggctgggg aatgggcatg
    52201 ttagccagcc ggtttgtcct tgaatggaat cactggattg cgtttgtgct gctgatattc
    52261 ctcggcgggc gaatgattat tgagggtttt cgtggcgcag atgatgaaga tgaagagccg
    52321 cgccgtcggc acggtttctg gctactggta accaccgcga ttgccaccag cctggatgcc
    52381 atggctgtgg gtgttggtct tgctttcctt caggtcaaca ttatcgcgac cgcattggcc
    52441 attggttgtg caaccttgat tatgtcgaca ttagggatga tggttggtcg ctttatcggc
    52501 tcaattattg ggaaaaaagc ggaaattctc ggcgggctgg tgctgatcgg catcggcgtc
    52561 cagatcctct ggacgcactt ccacggttaa taagaacgct gccagaggtg aatattaaag
    52621 tccgtctggc agtctaacac ttcttttgct gccagtattt gccagacttc tggcttcgca
    52681 cgccaggcaa acggcgtcat ctgcaataat gcgacggctt catcaccgcg aagacgcatc
    52741 ggataacaca actcatcact cttctgtaat gtaaaacctt ccagttgttc tgcatgaggt
    52801 gcatgaagat gtacttcatt gtaaatcagc cccttcagct ccatcaaatg tcgcggtccc
    52861 ggcgtggcag taatgaccca gccgccgggc ttcactactc gtgctaattc ttctgcttta
    52921 cacggcgcgt aaatacgtat tatggcgtcc atactggtat cggaaaacgg caaacggtgg
    52981 ctggaagcga cacaaagagt gacctgcgga tagcgtttcg ccgccgcttt tatcgctacc
    53041 ttcgaaacat ccagaccaaa cgtggtgatt tcgggcaacg catcggcaaa tgcgtgtgtg
    53101 taataccctt caccacagcc aatatccagc accgccgtgg ccttttcatt aagccgttcc
    53161 cgcagttggg cgacaattgc atcacgcagc ggctgataat gtccggcatc taagaatgcg
    53221 cggcgtgctt gcatcatttc cgcgctgtcg cccggatcac gagaccgttt atgctgaacg
    53281 acccttcttt cgccatatca aactgatgtc gttggggaca gatatagctg tttttttcac
    53341 gcgtaagagg ctgatggcaa agtggacagg aaaacgacat gacaactccg gcaggatact
    53401 aaaggccgca agtgtaacgc gaatcacgcg gcgggcaaat atccgaacgc caggagcgaa
    53461 gataaagcat agtaaaaagc ctcgcattcg acgaggcttt atagcgagat tgaagcgtat
    53521 tcacacttca gatcagtgga ttcgatcaga tagctgttac gttaacagct gccggacctt
    53581 tctggccgtc ctgaatttcg aactcaacgt tctgaccttc agccagagtt ttgaagccat
    53641 taccctggat agcggagaag tgtacgaaca cgtctttgct gccatcagca ggagtaatga
    53701 agccaaaacc tttagactcg ttgaaccact taacctgacc tttaatcttt gccatttgaa
    53761 aaattcctta gattgttttc ttcgcccgca ggcataacat agataaaact gacacattac
    53821 tgcatgaggc accaatataa ggctcggcag agaagcggta ttcaacgtca acgtgtttac
    53881 tcaggacttc tttactgaaa atgccacaca taaacagaac tgtacctcgt ttaacccgaa
    53941 atctgttatc acatacaacg ttaattatgg caagccattt ttaaacatgt ctcgatcaga
    54001 cgcacaaatc tcgatacgtc ttccactttt tttgcacact tatgcaacgg aatacgcgcc
    54061 gatatatcat tgtgcttaac cttgtcagtt ccgcagatac ttaaactgac gtagtttcta
    54121 acatagttca ataagttcag ttgttccagg tcagtgggag aagttattac atagtgcgtg
    54181 caatatcaca ttttctgcta tgcaatgaat aaaaagttat atcacttttt ctcataaaac
    54241 aggcagttaa cggctctcaa tcac
//
LOCUS       DABSYT020000020        52326 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.020, whole genome shotgun sequence.
ACCESSION   DABSYT020000020 DABSYT020000000
VERSION     DABSYT020000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 52326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.020"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..1304
                     /locus_tag="ID725_RS07975"
                     /old_locus_tag="pgaptmp_001595"
     CDS             <1..1304
                     /locus_tag="ID725_RS07975"
                     /old_locus_tag="pgaptmp_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGV7850224.1"
                     /translation="DTFIFTIIPDNNHVIKLSSPITVTIERKDGYYFINFSGDKSDII
                     YKVDGFSIKERNFFTLLSGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGV
                     INPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCDLLKHEDMASFAYENK
                     KLKKLFTLLDKRDMILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLN
                     SMRNMEGNKDSRINKNVVVLAGNHEINFNGNYSARLANHKLSSGDTYNLIKTLDVCNY
                     DSERHVLTSHHGIIRDEENKCYCLGALQVPFNQMKNPTDLEELAYIFNKKHKQHMDDH
                     LFHLIRSNTMAPTPVYADYLNNTTDFRPDPEDIFKCGQTLECGDPRKNIRQKYGHHGL
                     GVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPH"
     gene            1566..3188
                     /locus_tag="ID725_RS07980"
                     /old_locus_tag="pgaptmp_001596"
     CDS             1566..3188
                     /locus_tag="ID725_RS07980"
                     /old_locus_tag="pgaptmp_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGV7850225.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDYSEKYSMLSGMRAHILRR
                     NNHYQFIVTINSKNYGCPLDNTDINWCGIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQVDTNVNTIISILNRRDIILESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINDNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHTGRMFSMHNCQINPSA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3378..3491
                     /gene="yhiY"
                     /locus_tag="ID725_RS07985"
                     /old_locus_tag="pgaptmp_001597"
     CDS             3378..3491
                     /gene="yhiY"
                     /locus_tag="ID725_RS07985"
                     /old_locus_tag="pgaptmp_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGV7850226.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            3554..4621
                     /gene="yhiI"
                     /locus_tag="ID725_RS07990"
                     /old_locus_tag="pgaptmp_001598"
     CDS             3554..4621
                     /gene="yhiI"
                     /locus_tag="ID725_RS07990"
                     /old_locus_tag="pgaptmp_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGV7850227.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            4618..7353
                     /gene="rbbA"
                     /locus_tag="ID725_RS07995"
                     /old_locus_tag="pgaptmp_001599"
     CDS             4618..7353
                     /gene="rbbA"
                     /locus_tag="ID725_RS07995"
                     /old_locus_tag="pgaptmp_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGV7850228.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEASPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            7353..8477
                     /gene="yhhJ"
                     /locus_tag="ID725_RS08000"
                     /old_locus_tag="pgaptmp_001600"
     CDS             7353..8477
                     /gene="yhhJ"
                     /locus_tag="ID725_RS08000"
                     /old_locus_tag="pgaptmp_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7850229.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            8550..8825
                     /locus_tag="ID725_RS08005"
                     /old_locus_tag="pgaptmp_001601"
     CDS             8550..8825
                     /locus_tag="ID725_RS08005"
                     /old_locus_tag="pgaptmp_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGV7850230.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            8822..9181
                     /locus_tag="ID725_RS08010"
                     /old_locus_tag="pgaptmp_001602"
     CDS             8822..9181
                     /locus_tag="ID725_RS08010"
                     /old_locus_tag="pgaptmp_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGV7850231.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(9231..10091)
                     /locus_tag="ID725_RS08015"
                     /old_locus_tag="pgaptmp_001603"
     CDS             complement(9231..10091)
                     /locus_tag="ID725_RS08015"
                     /old_locus_tag="pgaptmp_001603"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HGV7850232.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(10123..10392)
                     /locus_tag="ID725_RS08020"
                     /old_locus_tag="pgaptmp_001604"
     CDS             complement(10123..10392)
                     /locus_tag="ID725_RS08020"
                     /old_locus_tag="pgaptmp_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGV7850233.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(10382..11890)
                     /locus_tag="ID725_RS08025"
                     /old_locus_tag="pgaptmp_001605"
     CDS             complement(10382..11890)
                     /locus_tag="ID725_RS08025"
                     /old_locus_tag="pgaptmp_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGV7850234.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(11883..13241)
                     /locus_tag="ID725_RS08030"
                     /old_locus_tag="pgaptmp_001606"
     CDS             complement(11883..13241)
                     /locus_tag="ID725_RS08030"
                     /old_locus_tag="pgaptmp_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGV7850235.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(13318..13599)
                     /locus_tag="ID725_RS08035"
                     /old_locus_tag="pgaptmp_001607"
     CDS             complement(13318..13599)
                     /locus_tag="ID725_RS08035"
                     /old_locus_tag="pgaptmp_001607"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGV7850236.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(13596..14069)
                     /locus_tag="ID725_RS08040"
                     /old_locus_tag="pgaptmp_001608"
     CDS             complement(13596..14069)
                     /locus_tag="ID725_RS08040"
                     /old_locus_tag="pgaptmp_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGV7850237.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(14094..14840)
                     /locus_tag="ID725_RS08045"
                     /old_locus_tag="pgaptmp_001609"
     CDS             complement(14094..14840)
                     /locus_tag="ID725_RS08045"
                     /old_locus_tag="pgaptmp_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGV7850238.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(15039..15440)
                     /gene="nikR"
                     /locus_tag="ID725_RS08050"
                     /old_locus_tag="pgaptmp_001610"
     CDS             complement(15039..15440)
                     /gene="nikR"
                     /locus_tag="ID725_RS08050"
                     /old_locus_tag="pgaptmp_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGV7850239.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(15446..16252)
                     /gene="nikE"
                     /locus_tag="ID725_RS08055"
                     /old_locus_tag="pgaptmp_001611"
     CDS             complement(15446..16252)
                     /gene="nikE"
                     /locus_tag="ID725_RS08055"
                     /old_locus_tag="pgaptmp_001611"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGV7850240.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(16249..17013)
                     /gene="nikD"
                     /locus_tag="ID725_RS08060"
                     /old_locus_tag="pgaptmp_001612"
     CDS             complement(16249..17013)
                     /gene="nikD"
                     /locus_tag="ID725_RS08060"
                     /old_locus_tag="pgaptmp_001612"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGV7850241.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(17013..17846)
                     /gene="nikC"
                     /locus_tag="ID725_RS08065"
                     /old_locus_tag="pgaptmp_001613"
     CDS             complement(17013..17846)
                     /gene="nikC"
                     /locus_tag="ID725_RS08065"
                     /old_locus_tag="pgaptmp_001613"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGV7850242.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(17843..18787)
                     /gene="nikB"
                     /locus_tag="ID725_RS08070"
                     /old_locus_tag="pgaptmp_001614"
     CDS             complement(17843..18787)
                     /gene="nikB"
                     /locus_tag="ID725_RS08070"
                     /old_locus_tag="pgaptmp_001614"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGV7850243.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(18787..20361)
                     /gene="nikA"
                     /locus_tag="ID725_RS08075"
                     /old_locus_tag="pgaptmp_001615"
     CDS             complement(18787..20361)
                     /gene="nikA"
                     /locus_tag="ID725_RS08075"
                     /old_locus_tag="pgaptmp_001615"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGV7850244.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(20472..21059)
                     /gene="acpT"
                     /locus_tag="ID725_RS08080"
                     /old_locus_tag="pgaptmp_001616"
     CDS             complement(20472..21059)
                     /gene="acpT"
                     /locus_tag="ID725_RS08080"
                     /old_locus_tag="pgaptmp_001616"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGV7850245.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(21061..22290)
                     /locus_tag="ID725_RS08085"
                     /old_locus_tag="pgaptmp_001617"
     CDS             complement(21061..22290)
                     /locus_tag="ID725_RS08085"
                     /old_locus_tag="pgaptmp_001617"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HGV7850246.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(22287..23018)
                     /locus_tag="ID725_RS08090"
                     /old_locus_tag="pgaptmp_001618"
     CDS             complement(22287..23018)
                     /locus_tag="ID725_RS08090"
                     /old_locus_tag="pgaptmp_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HGV7850247.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(23018..23482)
                     /locus_tag="ID725_RS08095"
                     /old_locus_tag="pgaptmp_001619"
     CDS             complement(23018..23482)
                     /locus_tag="ID725_RS08095"
                     /old_locus_tag="pgaptmp_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HGV7850248.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(23479..24648)
                     /locus_tag="ID725_RS08100"
                     /old_locus_tag="pgaptmp_001620"
     CDS             complement(23479..24648)
                     /locus_tag="ID725_RS08100"
                     /old_locus_tag="pgaptmp_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HGV7850249.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(24650..25234)
                     /locus_tag="ID725_RS08105"
                     /old_locus_tag="pgaptmp_001621"
     CDS             complement(24650..25234)
                     /locus_tag="ID725_RS08105"
                     /old_locus_tag="pgaptmp_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HGV7850250.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(25231..27549)
                     /locus_tag="ID725_RS08110"
                     /old_locus_tag="pgaptmp_001622"
     CDS             complement(25231..27549)
                     /locus_tag="ID725_RS08110"
                     /old_locus_tag="pgaptmp_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HGV7850251.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(27518..28123)
                     /locus_tag="ID725_RS08115"
                     /old_locus_tag="pgaptmp_001623"
     CDS             complement(27518..28123)
                     /locus_tag="ID725_RS08115"
                     /old_locus_tag="pgaptmp_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HGV7850252.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(28120..28542)
                     /locus_tag="ID725_RS08120"
                     /old_locus_tag="pgaptmp_001624"
     CDS             complement(28120..28542)
                     /locus_tag="ID725_RS08120"
                     /old_locus_tag="pgaptmp_001624"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HGV7850253.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(28546..30222)
                     /locus_tag="ID725_RS08125"
                     /old_locus_tag="pgaptmp_001625"
     CDS             complement(28546..30222)
                     /locus_tag="ID725_RS08125"
                     /old_locus_tag="pgaptmp_001625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGV7850254.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(30213..30566)
                     /locus_tag="ID725_RS08130"
                     /old_locus_tag="pgaptmp_001626"
     CDS             complement(30213..30566)
                     /locus_tag="ID725_RS08130"
                     /old_locus_tag="pgaptmp_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HGV7850255.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(30553..31914)
                     /locus_tag="ID725_RS08135"
                     /old_locus_tag="pgaptmp_001627"
                     /pseudo
     CDS             complement(30553..31914)
                     /locus_tag="ID725_RS08135"
                     /old_locus_tag="pgaptmp_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
     gene            complement(31911..32492)
                     /locus_tag="ID725_RS08140"
                     /old_locus_tag="pgaptmp_001628"
     CDS             complement(31911..32492)
                     /locus_tag="ID725_RS08140"
                     /old_locus_tag="pgaptmp_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850256.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(32497..32748)
                     /locus_tag="ID725_RS08145"
                     /old_locus_tag="pgaptmp_001629"
     CDS             complement(32497..32748)
                     /locus_tag="ID725_RS08145"
                     /old_locus_tag="pgaptmp_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGV7850257.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(32760..33017)
                     /locus_tag="ID725_RS08150"
                     /old_locus_tag="pgaptmp_001630"
     CDS             complement(32760..33017)
                     /locus_tag="ID725_RS08150"
                     /old_locus_tag="pgaptmp_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HGV7850258.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(32992..33813)
                     /locus_tag="ID725_RS08155"
                     /old_locus_tag="pgaptmp_001631"
                     /pseudo
     CDS             complement(32992..33813)
                     /locus_tag="ID725_RS08155"
                     /old_locus_tag="pgaptmp_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
     gene            complement(33810..34532)
                     /locus_tag="ID725_RS08160"
                     /old_locus_tag="pgaptmp_001632"
     CDS             complement(33810..34532)
                     /locus_tag="ID725_RS08160"
                     /old_locus_tag="pgaptmp_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HGV7850259.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(34573..35653)
                     /locus_tag="ID725_RS08165"
                     /old_locus_tag="pgaptmp_001633"
                     /pseudo
     CDS             complement(34573..35653)
                     /locus_tag="ID725_RS08165"
                     /old_locus_tag="pgaptmp_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(35722..36099)
                     /locus_tag="ID725_RS08170"
                     /old_locus_tag="pgaptmp_001634"
     CDS             complement(35722..36099)
                     /locus_tag="ID725_RS08170"
                     /old_locus_tag="pgaptmp_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGV7850260.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(36491..37540)
                     /gene="yhhT"
                     /locus_tag="ID725_RS08175"
                     /old_locus_tag="pgaptmp_001635"
     CDS             complement(36491..37540)
                     /gene="yhhT"
                     /locus_tag="ID725_RS08175"
                     /old_locus_tag="pgaptmp_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGV7850261.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPIIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            37672..38889
                     /gene="yhhS"
                     /locus_tag="ID725_RS08180"
                     /old_locus_tag="pgaptmp_001636"
     CDS             37672..38889
                     /gene="yhhS"
                     /locus_tag="ID725_RS08180"
                     /old_locus_tag="pgaptmp_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850262.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(38893..39450)
                     /gene="dcrB"
                     /locus_tag="ID725_RS08185"
                     /old_locus_tag="pgaptmp_001637"
     CDS             complement(38893..39450)
                     /gene="dcrB"
                     /locus_tag="ID725_RS08185"
                     /old_locus_tag="pgaptmp_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGV7850263.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(39523..40188)
                     /gene="yhhQ"
                     /locus_tag="ID725_RS08190"
                     /old_locus_tag="pgaptmp_001638"
     CDS             complement(39523..40188)
                     /gene="yhhQ"
                     /locus_tag="ID725_RS08190"
                     /old_locus_tag="pgaptmp_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGV7850264.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            40409..40654
                     /gene="tusA"
                     /locus_tag="ID725_RS08195"
                     /old_locus_tag="pgaptmp_001639"
     CDS             40409..40654
                     /gene="tusA"
                     /locus_tag="ID725_RS08195"
                     /old_locus_tag="pgaptmp_001639"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGV7850265.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(40756..42954)
                     /gene="zntA"
                     /locus_tag="ID725_RS08200"
                     /old_locus_tag="pgaptmp_001640"
     CDS             complement(40756..42954)
                     /gene="zntA"
                     /locus_tag="ID725_RS08200"
                     /old_locus_tag="pgaptmp_001640"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGV7850266.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(43028..43654)
                     /gene="yhhN"
                     /locus_tag="ID725_RS08205"
                     /old_locus_tag="pgaptmp_001641"
     CDS             complement(43028..43654)
                     /gene="yhhN"
                     /locus_tag="ID725_RS08205"
                     /old_locus_tag="pgaptmp_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGV7850267.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            43795..44154
                     /gene="yhhM"
                     /locus_tag="ID725_RS08210"
                     /old_locus_tag="pgaptmp_001642"
     CDS             43795..44154
                     /gene="yhhM"
                     /locus_tag="ID725_RS08210"
                     /old_locus_tag="pgaptmp_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGV7850268.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(44157..44426)
                     /gene="yhhL"
                     /locus_tag="ID725_RS08215"
                     /old_locus_tag="pgaptmp_001643"
     CDS             complement(44157..44426)
                     /gene="yhhL"
                     /locus_tag="ID725_RS08215"
                     /old_locus_tag="pgaptmp_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGV7850269.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(44416..45012)
                     /gene="rsmD"
                     /locus_tag="ID725_RS08220"
                     /old_locus_tag="pgaptmp_001644"
     CDS             complement(44416..45012)
                     /gene="rsmD"
                     /locus_tag="ID725_RS08220"
                     /old_locus_tag="pgaptmp_001644"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGV7850270.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            45162..46658
                     /gene="ftsY"
                     /locus_tag="ID725_RS08225"
                     /old_locus_tag="pgaptmp_001645"
     CDS             45162..46658
                     /gene="ftsY"
                     /locus_tag="ID725_RS08225"
                     /old_locus_tag="pgaptmp_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGV7850271.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            46661..47329
                     /gene="ftsE"
                     /locus_tag="ID725_RS08230"
                     /old_locus_tag="pgaptmp_001646"
     CDS             46661..47329
                     /gene="ftsE"
                     /locus_tag="ID725_RS08230"
                     /old_locus_tag="pgaptmp_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGV7850272.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            47322..48380
                     /gene="ftsX"
                     /locus_tag="ID725_RS08235"
                     /old_locus_tag="pgaptmp_001647"
     CDS             47322..48380
                     /gene="ftsX"
                     /locus_tag="ID725_RS08235"
                     /old_locus_tag="pgaptmp_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGV7850273.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            48625..49479
                     /gene="rpoH"
                     /locus_tag="ID725_RS08240"
                     /old_locus_tag="pgaptmp_001648"
     CDS             48625..49479
                     /gene="rpoH"
                     /locus_tag="ID725_RS08240"
                     /old_locus_tag="pgaptmp_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGV7850274.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            49750..50853
                     /gene="livJ"
                     /locus_tag="ID725_RS08245"
                     /old_locus_tag="pgaptmp_001649"
     CDS             49750..50853
                     /gene="livJ"
                     /locus_tag="ID725_RS08245"
                     /old_locus_tag="pgaptmp_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGV7850275.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            51003..51269
                     /gene="ataR"
                     /locus_tag="ID725_RS08250"
                     /old_locus_tag="pgaptmp_001650"
     CDS             51003..51269
                     /gene="ataR"
                     /locus_tag="ID725_RS08250"
                     /old_locus_tag="pgaptmp_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HGV7850276.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            51276..51803
                     /gene="ataT"
                     /locus_tag="ID725_RS08255"
                     /old_locus_tag="pgaptmp_001651"
     CDS             51276..51803
                     /gene="ataT"
                     /locus_tag="ID725_RS08255"
                     /old_locus_tag="pgaptmp_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HGV7850277.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(51800..52183)
                     /gene="panM"
                     /locus_tag="ID725_RS08260"
                     /old_locus_tag="pgaptmp_001652"
     CDS             complement(51800..52183)
                     /gene="panM"
                     /locus_tag="ID725_RS08260"
                     /old_locus_tag="pgaptmp_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGV7850278.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT        12664 a        13475 c        14007 g        12180 t
ORIGIN      
        1 ctgacacgtt tatctttaca attataccag acaataacca tgtgataaaa ttatcgtctc
       61 ccattaccgt tacgattgag cgtaaggacg gttattattt tattaatttc tccggggata
      121 aatcagacat aatttataaa gttgatgggt tttctatcaa agagagaaat ttctttactc
      181 ttcttagtgg taatttcaaa cctgactggc gttgggctgt ctctaaagag acgtttacta
      241 aggaaaagtt tgatagctat gttgaatctg ttttttcaaa aatagacttt tataaacagt
      301 gtggtgtgat taacccacaa aatgccaaca cggcttattt tggcgataca gatggaagag
      361 taggcgctgt actttatgca ttgctcgttt cagggcatat aggcatcagg gaaaaggggt
      421 ggagtttatt atgtgattta ttgaaacacg aagacatggc ttcattcgca tatgaaaata
      481 aaaaattgaa aaaactcttc actttattag ataagagaga catgatcttg aatgagttgc
      541 atcaacatgt ttttttaaaa ggcgatgcga ttaccccttg tatcttcctt ggggatcata
      601 caggcgatag attcagtact atttttggtg ataaatatat ccttactttg ctaaattcta
      661 tgcgaaatat ggagggtaat aaagatagtc gtataaataa aaatgtcgtt gttctggctg
      721 gaaatcatga aatcaatttt aatggtaact atagtgcacg actagcaaat cataaactct
      781 cttccggtga tacatataac ttaattaaaa cgctcgatgt ttgtaattat gactcagaaa
      841 ggcatgtttt aaccagtcat cacggaataa taagggatga agaaaataaa tgttattgtc
      901 tcggtgcttt gcaggttcca tttaatcaga tgaaaaatcc tactgatctt gaggaattag
      961 catatatatt taataaaaaa cataaacaac atatggatga tcatcttttc catctaatta
     1021 gaagcaatac tatggcaccc acacctgtat atgctgatta tttaaataac actactgatt
     1081 tcagaccaga tcctgaagat atcttcaaat gtggacagac gctcgaatgt ggcgaccccc
     1141 gcaaaaacat tagacaaaaa tatggccatc atgggttagg ggtaaatcag aatcaacaat
     1201 ttgataatgg tgttatgggg ctaaattcca ggatggcagt gtttgatgaa aataaaaaaa
     1261 taataggttc ttcctcagga ctaagttgtt ttcaaccaca ttaaaactct atgagtgctg
     1321 tggtatatct ttttaatcct gccagtattt catagttttt cttaatcgat tgtagaggca
     1381 tgtactgatg acgcctctac aatctttagg gactttctct actatgagat aagaaatttc
     1441 atattatgct ttaaggctga atgatttgtg caaaatataa ttatttttcc atcgaacatg
     1501 aggtgtatgt cataagatta aatgataact atgtattcct cgcggtgaat tatatgaggt
     1561 aaaagatgaa aattggaact gtggcaggta ctaacgacag tacaacgaca atcgcaacaa
     1621 atgatatggt gcaagagcat gttactaact tcactaaaga actgtttggg tatattgcaa
     1681 atggtatagg tgatgatata tcaagtattg ccaggacaat gctcggtgag gttctggaaa
     1741 aagtagatga ttggcaaata gaacggttcc aacaatctat tcaagatgac aaaatttcat
     1801 ttaccataca aacggattat tcagaaaaat atagtatgct atcgggcatg cgtgctcata
     1861 ttctacgtag gaataaccat tatcagttta ttgtgacgat taatagcaaa aattacggtt
     1921 gccctcttga taataccgat attaattggt gcggcattgt ttatttactt aataatatga
     1981 cagtaaatga taatgctaat gatgttgcag ttactgagtc gtacaaaccc atctggaact
     2041 ggaaaatatc gcaatacaat gtatctgata ttaaattcga aacaattata aaaccccagt
     2101 tcgctgacag aatctatttc tccaattgtt cacctgttga tcccacaagt accagaccaa
     2161 cctattttgg tgataccgat ggctcagttg gtgcagtttt gtacgcgctt tttgcaacgg
     2221 gacatttggg aatcatggct gaaggagaaa atttccttag ccagttgctt aatatagaag
     2281 atgaagttct caatgtattg ctaagggaga actttaatga gcaagttgat actaatgtta
     2341 atacaataat cagtatcctg aacagaagag acattatttt agaaagtctt cagccttatt
     2401 tagttattaa taaagacgca gtgacgccat gtacgttttt gggtgaccaa accggcgatc
     2461 gatttagtaa tatctgtggc gatcaattta tcatcgattt gttaaaacgc ataatgagca
     2521 tcaatgataa cgttcatgta ttagctggaa atcatgaaac gaattgcaat ggtaactata
     2581 tgcagaactt cacgcgtatg aaaccacttg atgaggatac ctacgacggg ataaaagatt
     2641 atcctgtatg tttctatgac tccaaataca aaataatggc aaatcatcat gggataactt
     2701 ttgatgatca gcgaaaacgt tacattatag gaccgataac ggtctctatt gatgaaatga
     2761 ccaatgctct cgatccggtc gaattagcgg caattattaa taaaaagcat catactataa
     2821 ttaatagtaa aaagtttaaa actagccggg ctatctcttg tcgatccttt aatcgttatt
     2881 tttctgtttc gacagattac agacctaaac ttgaagcact attagcatgc tcacaaatgt
     2941 taggtataaa tcaagttgtt gcacataacg gtaatggtgg aagagaatgc ataggtgaaa
     3001 cggggacagt tttaggtctt aacgcccgtg acagtaaaca tacgggaaga atgttcagca
     3061 tgcataattg ccaaattaac cctagtgcgg ggcctgagat aaccactccc tggaagtctt
     3121 accaacatga aaaaaacaga aatggactta tgcctttgat tagaaggcgc acaatgttac
     3181 agctctgaac catagacaac atgaatatta atgttactat ctggttgaga ctgttgataa
     3241 aacgtaataa ggaatgctct gttcctgcaa ctgaacaggc gtgaaacgct atccaacagg
     3301 atggataccg ttttgtcagc cagttttata gatcacactt atgagtatgc gtgtttcatt
     3361 tacggccagg aatgctgatg atgacaacat tactacccgt tttcactaag ccttccccgt
     3421 tggcgctcaa tgctctgcgc gctggtcgta tttgtcgttt ccttcttatc ccggatggaa
     3481 gaatccgctg aacgtcgttc ggcggcagtt gtaacacgcg tcgtttgctt acagggatta
     3541 tggcgggtcg attatggata agagtaagcg ccatctggcg tggtggggtg tcgggttact
     3601 ggcggtggct gctatcgtag cgtggtggct gttgcgcccg gcaggtgtgc cggaaggctt
     3661 tgccgtcagc aatgggcgca ttgaagcgac ggaagtggat atcgccagca aaattgccgg
     3721 gcgtatcgac accattctgg tgaaagaagg ccagtttgtt cgcgaaggcg aagtgctggc
     3781 gaagatggat actcgcgtgt tgcaggagca gcgactggaa gccatcgcgc aaatcaaaga
     3841 ggcacaaagc gccgttgccg ccgcgcaggc tttgctggag caacgacaaa gcgaaactcg
     3901 cgccgcacag tcgctggtta atcaacgcca ggccgaactg gactccgttg ccaaacgtca
     3961 tacgcgttcc cgctcgctcg cccaacgagg ggctatttct gcgcaacagc tggatgacga
     4021 tcgcgccgcc gctgagagcg cccgagctgc gctggaatca gcaaaagccc aggtttcggc
     4081 ttctaaagcc gctatagaag cggcacgcac caatatcatt caggcgcaaa cccgcgtcga
     4141 agccgcacaa gccactgaac ggcgcattgc cgcagatatc gatgacagcg aactgaaagc
     4201 cccgcgtgac ggacgcgtgc agtatcgggt tgccgagcca ggggaagtgc tggcggcagg
     4261 cggtcgggtg ctgaatatgg tcgatctcag cgacgtctat atgaccttct tcctgccaac
     4321 cgaacaggcg ggcacgctga aacttggcgg tgaagcccgg ctgatcctcg atgccgcgcc
     4381 agatctgcgt attcctgcaa ccatcagttt tgtcgccagt gtcgcccagt tcacgccaaa
     4441 aaccgtcgaa accagcgatg aacggctgaa actgatgttc cgcgtcaaag cgcgtatccc
     4501 accggaatta ctccagcagc atctggaata tgtcaaaacg ggcttgccgg gcgtagcgtg
     4561 ggtgcgggtg aatgaagaac ttccgtggcc tgacgacctc gtggtgaggt tgccgcaatg
     4621 acgcatctgg aactggttcc cgtcccgcct gtcgcgcaac tggcgggcgt gagccagcat
     4681 tatggaaaaa ccgttgcgct gaacaatatc acactcgata ttccggcccg ctgtatggtc
     4741 gggctgattg gtccggatgg cgtcgggaag tcgagtttat tgtcattgat ttccggtgct
     4801 cgtgtcattg agcagggcaa tgtgatcgtg ctgggcggcg atatgcgcga cccgaagcat
     4861 cgccgcgacg tctgcccgcg catcgcctgg atgccgcagg ggctgggcaa aaacctctac
     4921 cacaccttgt cggtgtatga aaacgtcgat tttttcgctc gcctgttcgg tcacgacaaa
     4981 gcggagcggg aagtgcgaat caatgagctg ctgaccagca ccgggttagc accgtttcgc
     5041 gatcgtccgg cagggaaact ctccggcggg atgaagcaaa aactggggct gtgctgtgcg
     5101 ttaatccacg acccggaact gttgattctt gatgagccta cgacgggggt tgacccgctc
     5161 tcccgcgccc agttctggga tctgatcgac agtattcgcc agcggcagag caatatgagc
     5221 gttctggtcg ccacggccta tatggaagag gcggaacgct tcgactggtt ggtggcgatg
     5281 aatgccggag aagtgttggc aactggcagc gccgaagagc tgcggcagca aacgcaaagc
     5341 gccacgctgg aagaagcatt tataaatctg ttaccgcaag cgcaacgcca ggcgcatcag
     5401 gcggtagtga tcccaccata tcaacctgaa aacgcagaga ttgccatcga agcgcgcgat
     5461 ctgaccatgc gttttggttc cttcgttgcc gttgatcacg tcaatttccg cattccacgc
     5521 ggggagattt ttggttttct tggttcgaac ggctgcggta aatccaccac catgaaaatg
     5581 cttaccggat tgctgcccgc cagcgaaggt gaggcgtggc tgttcgggca accggttgat
     5641 ccaaaagata tcgatactcg ccgtcgggtg ggctatatgt cgcaggcgtt ttcgctctat
     5701 aacgagctca ccgtgcggca aaaccttgag ttacatgccc gtttgtttca catcccggaa
     5761 gcggaaattc ccgcaagagt ggctgaaatg agcgagcgtt ttaagctcaa cgacgttgaa
     5821 gatgttctgc cggagtcatt gccgctcggc attcgccagc ggctttcgct ggcggtggcg
     5881 gtgattcatc gcccggaaat gttaatcctc gatgaaccta cttctggcgt cgatccggtg
     5941 gcgagggata tgttctggca gttgatggtc gatctctcgc gccaggacaa agtgactatc
     6001 ttcatctcca cccactttat gaacgaagcg gaacgctgcg accgtatctc tctgatgcac
     6061 gccggaaaag tgctcgccag cggtacaccg caggaactgg ttgagaaacg cggagccgcc
     6121 agtctggaag aggcatttat cgcctatttg caggaagcgg caggacagag caacgaagcc
     6181 gaagcgtctc ccgtgataca cgacaccaca cacgcgccgc gccagggatt tagcctgcgc
     6241 cgtctgttta gctacagccg ccgcgaagcg ctggaactgc gacgcgatcc ggtacgttcg
     6301 acgctggcgc tgatgggaac agtgatcctg atgctgataa tgggttacgg catcagtatg
     6361 gatgtggaaa acctgcgctt tgcggtgctt gaccgcgacc agaccgtcag tagccaggcg
     6421 tggacgctca atctctccgg ttcccgttac tttatcgaac agccgctgct taccagttat
     6481 gacgagcttg atcgtcggat gcgtgcgggc gatatcacgg tggcaattga gatcccgccc
     6541 aatttcgggc gcgatatcgc gcgtggtacg cctgtggaac tcggtgtctg gatcgacggt
     6601 gcgatgccga gccgcgccga aactgtaaaa ggttacgtgc aggccatgca ccagagctgg
     6661 ctacaggatg tggcgagccg acaatccaca ccctccagcc aaagcgggct gatgaatatt
     6721 gagacgcgtt atcgctataa cccggacgta aaaagcctgc cagcgattgt tccggcggtg
     6781 atcccgcttc tgctgatgat gatcccgtca atgctaagcg cccttagcgt ggtgcgggaa
     6841 aaagagctag ggtcgattat caacctttac gtgaccccca ccacccgtag cgaatttttg
     6901 cttggcaaac agctgccgta catcgcgctg gggatgctga actttttcct gctctgcggc
     6961 ctgtcggtgt ttgtgtttgg cgtaccgcat aaaggcagtt tcctgacgct caccctggcg
     7021 gcgctgctgt atatcatcat cgccaccgga atggggctgc tgatctccac ctttatgaaa
     7081 agccagattg ccgccatttt cggtacggcg attatcacgt tgatcccggc gacacagttt
     7141 tccgggatga tcgatccggt agcttcgctg gaagggccgg gacgttggat cggcgaggtt
     7201 tacccgacca gtcattttct gactatcgcc cgcgggacgt tctcgaaagc gctggatctg
     7261 actgatttgt ggcaactttt tatcccgttg ctgatagcca taccgctggt gatgggctta
     7321 agcatcctgc tgctgaaaaa acaggaggga tgatgcgcca tttacgcaat atttttaatc
     7381 tgggtatcaa agagttgcgc agtctactcg gtgataaagc gatgctgaca ttgattgtct
     7441 tctcgtttac ggtgtcggtg tattcgtcag cgaccgtcac gccaggatcg ttgaacctgg
     7501 caccgatcgc cattgccgat atggatcaat cgcagttatc gaaccggatc gttaacagct
     7561 tctatcgccc gtggtttttg ccaccggaga tgatcaccgc cgatgagatg gatgccggac
     7621 tggacgccgg gcgttatacc tttgcgataa atattccgcc taattttcag cgtgatgtcc
     7681 tcgccggacg ccagccagat attcaggtga acgtcgatgc cacgcgcatg agccaggcat
     7741 ttaccggcaa tgggtatatc cagaatatta tcaacggtga agtgaacagc tttgtcgcgc
     7801 gctaccgtga taacagcgaa ccgttggtat cgctggaaac ccggatgcgc tttaacccga
     7861 accttgatcc cgcgtggttt ggcggggtga tggcgatcat caacaacatt accatgctgg
     7921 cgattgtatt gaccggatcg gcgctgatcc gcgagcgtga acacggcacg gtggaacact
     7981 tactggtgat gccgataacg ccgtttgaga tcatgatggc gaagatctgg tcgatggggt
     8041 tggtggtgct ggtggtatcg ggattatcac tggtgctgat ggtaaaaggc gtgttgggcg
     8101 taccgattga aggctcgatc ccgctgttta tgctgggcgt ggcgcttagt ctgtttgcca
     8161 ccacgtcaat cggcattttt atggggacaa tagcgcgttc aatgccgcaa ctggggctgc
     8221 tggtgattct ggtgctgctg ccgctgcaaa tgctttccgg tggttccacg ccgcgcgaaa
     8281 gtatgccgca gatggtgcag gacattatgc tgatcatgcc gacgacacac tttgttagcc
     8341 tcgcgcaggc cattctctac cgtggtgccg gattcgaaat cgtctggccg cagttcctga
     8401 cgctgatggc aattggcggc gcgtttttca ccattgcgct gctgcgattc aggaagacga
     8461 ttgggacaat ggcgtaagga agggaaactt gacttcatac tgtgatgtca tactattgat
     8521 gtcataaagt gaagtcataa ggacatgcca tgagaaccaa ggtacaggct ttacggaaga
     8581 aacaaaaaaa tacattggat caaatattta aaactcctgt tcctcaaggg atcaagtggt
     8641 ctgatataga gtcactggtt aaagcattag gcggagaaat taaggaagga agaggttcgc
     8701 gttgtaagtt catactaaat atgagcgttg cgtgtttcca tcggcctcat ccgtcgccag
     8761 ataccgataa aggcgctgta gaaagcgtgc gtgactggtt actaagtata ggggtaaaac
     8821 catgatcaaa ctgaagacgc caaactctat ggagattgcc gggcaacctg ctgttatcac
     8881 ttacgtgcca gaactgaatg catttcgtgg caaattcctg ggcttgtcag gttattgtga
     8941 ttttgtctct gacagcattc aggggctgca aaaagaaggg gaactttccc tgcgagagta
     9001 tcttgaggat tgtaaagctg cgggtattga gccgtatgcg cgtacagaaa agattaagac
     9061 ctttacgtta cgctatccag aatcgctgag tgaacgcctt aataatgcgg ctgcgcagca
     9121 acaagtctct gttaatacct atattattga aacactgaat gagcgtttga atcatttata
     9181 acttatagcg gcccaacaat ttctgttgcg ccgctatatt tttgtggctc ttaagatgcc
     9241 tttctaacca accgatgccc ctcattcatc atcaggattt tgctatacac atcttcctcg
     9301 acggcgtttt ttgctgatgc catgactcgt gccagattcg cctcgttgtg attattcaca
     9361 tacgctttgc cgacggcggt gataaacgct ttgcgcagat cgctggcgat atttacttta
     9421 gcgacgcgat aattaacaaa gctgcgtaat atctccgggg cgataccgga gccgccgtga
     9481 ataaccaacg gcaccggaca gacttcggca atgcgcttca gcagggggat atcgatacgc
     9541 ggtatatcgt ccaggccatg aacgttgcca atagaaactg ccagcatgtc gcaaccggta
     9601 cgctcgacaa aggtcttcac cttttccggt tccgttttgc aatcggcttc gctgacgtga
     9661 tcatcttctt tgccgagaat cgcccccagc tccgcttcaa ccggtacgcc ataggattta
     9721 cagaaatcga cagcttcctg ggtgaaagcg atattctctt caaaagagaa tgcggcaccg
     9781 tcaatcatga ccgaggtaaa gcccgcgcgc accgcctgtt taacatcctc ctgggttttc
     9841 ccgtgatcca gatgaagact gaccggaaca tccattttat cggcgtgacg tttcacgatt
     9901 tcataaatgt aatcgtatcc cgagagttgt gcattggttg gcgcaatctg aatgaagttg
     9961 ggtaatcccg aacgctcaat agcgtttaaa attgaaattg tcgtctccag attggtggta
    10021 ttaaatgcgc ccgctaatac ccgtttttcc tgaatgcggt cgagcagtat ccgtccatta
    10081 actaatggca tattgaaaac ctcatctttt aatatcagtt aattattcag cttcaccgaa
    10141 gcggtcgcga aataagtcgg tgagagtctg tatggcctcg ttttcatcat caccgtcgca
    10201 gcgtatttcc accgtttttc cgccttttat tccggcgcgc ataatattca tcatgctggt
    10261 ggcattaata tcattaccct catagtgcag cgtcacttta cagctatatt ttttggcggc
    10321 tttcgccaga caggccgccg gacgtgcatg caggccagta ctgtttctga cctccacggt
    10381 tttattcaac atagtgttcc tctcgatgat gtaatggcat cacgccttct tgttgtaatt
    10441 tcaactgata ccagcttttc catttgtgct gataacgctg taattgatgg tatctcgccc
    10501 gattcggtaa caaacgttgg gctatagcga ctggcggcgg tggaatatcc ggtgcggcgg
    10561 ttaataatgc tccgacagaa accgcctcgt taaatgcaat tgcttcaacc ggacgctgta
    10621 aaatatccgc ctttaattgc agccaaagag gattatgggt tgccggaccg atcactttta
    10681 acgcgctggt gccaggcacg ttaaaacagg cgaagcagtt ggcaaattcc atggttaatc
    10741 ccagtgatac gctggcaatt aacatgtcga tacttaacgt atcgccaagc ccataaaata
    10801 atccacgggt atggcgattt ttatagggcg aacccgaacc gcgaagatgt ggaataaaga
    10861 cgggaatgtc atcgagcaac caattccccg ccagataatc agcatgtccg cgagtaagtg
    10921 atgcggcgat ctcctcatcg gttagccgga acgtgttgcg cagccactcc agcgcgaaac
    10981 cgcccacggg cagcgacgca aacagggtga actggttggc aagtgagtaa cagccgtttg
    11041 ccagcttgtc acgtttggcc tgttcatcca gcgtcggttg tgtatccagt tgcagaatgc
    11101 cttccgtggt ccccgtcgag ttaaggatat cgcctggcat catctgcaac gctcgcgcac
    11161 ccaccatatg gtcatgtccg gccagcgtca cgctgaccgg ttgcgagaac ccaagcgtct
    11221 tgcaaagtga ctcgctgacc catccggcgt gctcgcctgg ctgcaccaat ggcgcaaatg
    11281 ccgtacaggg aacgtgtaac aacgccgccg ctttcgctga ccattcgcct ttgcgcacgt
    11341 ccagacacag cgtgcggctg gctaaggtga tatccgtgtg ctgttcgcca gtcagccgcc
    11401 agagcagcac ttccggcgca tgtagccagc gcaggccgcg gcgattacgc agcgggtaat
    11461 gttccagcaa ccagcgcatt ttgaaggcag agtaattgct gtgtagtggt agaccgcaga
    11521 tgtcataaag tgccgcactg tctgcctcgc taagcgttgc cagatactct tcaccgcgac
    11581 ggtcatacca tgccagcatt ggcgtcagga tctcgccatg ctcgtcaagg aacacgcctg
    11641 attcgccaaa actggcaatg ctgatccgtc tgactggcag cggcgaagcg gcgttcagtt
    11701 gcgctatcgc ctggcggacc tcctgccaga gggcgtcgat atcgaaatcg acatcgccct
    11761 gtggggagat ctgctttgcc gtcacgaatt tatgcgcgcc caacgtcgtt gcgtccaggc
    11821 aggaaaagca ggtgactttg caattggtgg tgccaatatc gataacgata gctgcgctgt
    11881 tatcaggcat aaaccgtgct ccgcgtcatt cggaaaatgc catacagtac cagggcgata
    11941 accggcacca gaccaatggc gatgagattg ccgttgaaga tttccagcag ccagaagcgg
    12001 aacgggttac cgccatcaag aaaactggaa acctggccgc tggagccttc ggcaaatttg
    12061 aagttaacgt ctttcgccat tccggtaata aacggcgcga ttttggtagc aatccatagg
    12121 tcggcaataa tcaccgggat cgccgccaga acggtgcgga aaatattgcc acggctggct
    12181 aaagcaatca tcgacgccat taccgccagg ttggcaaggt cgccaagcgg caggacttca
    12241 ttacccggaa ggacaaacgc gataaagacc gagattggcg ttagcagcag ggcggtggag
    12301 ataatcgccg gattacctac ggcaacggcg atatccaggc cgatatagag atcgtcacgg
    12361 tcagggtatt tggcattgag atacttttta atagcttcag acagcggtaa taaaccttcc
    12421 atcaggatgc gtaccatgcg tggcaggatg aacatcacac cgccaatgct gatgccaagc
    12481 agcaatactt ttttgaaatc gtatccggca attacgccga gcagaatgcc cagaatagtg
    12541 ccaaccatca tcggttcgcc gaagatgcca aactttttct gtacggtttc cgggtcgata
    12601 tgaatacgat tgaggccagg gatatggtcg atgattttgt cgaccagcag accgaccggg
    12661 aagaacacca ccgaagagag cgtcggcaat gagatccctt ccagtccaaa gtatttttgt
    12721 accagtggcg cagaccagtc ggcgagttta agtactacca ccgcagcaat cgccgccgcc
    12781 agcaaaccaa agaacaggct gccggtgctg taataaacca acgtaccagc aagggcaaaa
    12841 tgccagtagt tccacatatc tacgtcaacg gtacgggttt tatttaacgc cagcatcgcc
    12901 acgttaatca gaatggtcag gggaataaca aacggggcaa ttggcgaacc ccatgtaata
    12961 gcggaaagcg gcggccagcc taaatctacc accggtaaat taattccggt gcgttcaacc
    13021 atcgcctggg ccgctggccc gacattgctg gtcagaatat cgaaaatggc ataaataccg
    13081 acaaaaccaa tgccgactgt taatgacgaa cgtaaggctt tagcgggttt tactttaaaa
    13141 cagagagcaa gaataaataa caccaacggc aacattaccg tcgggccaaa tcccaatata
    13201 tattgcacaa tattatagag ggtatgcgcg atatcgttca taataaacct ctttttcagg
    13261 cgagagcgca cctcgcgtta gcgcctgatg gagtgggttg aaattaatta ttattaatta
    13321 ctttttaagg atagaaacaa tttgcgccag tactttttct tcgccaatac cggtaataat
    13381 cggcaagccg ttcaccaccg ggacgtcgag ttcataaggc actttagttg ttgaaactac
    13441 cagtgctacg ccgtcagtat ttccaggtaa agaggcaacg ttggtttggg aataattgac
    13501 gttaagacct tgctctttgc aatattccat gactttttct gttaccgccg tggatgtggc
    13561 aatacccgtg gcgcagacaa ataaaatggt ttgactcata tttcacctcg ttctttcatt
    13621 ttgtttaaga ttactgtgtg tatttctgtt gtttccatcg caagcagggc ttgcataaag
    13681 ggcgtatcct gaatcatttc cataatccag cccagggcat ttaattgttt attgctttcg
    13741 cttaaggcga gcaaaaaaat caccctgaca ggtacggggt caggatcgcc gcccatatcg
    13801 gcaaactcta ccggttccgg caggatgccg acggcaattg cattgtgcct gacatgttta
    13861 tggtccgtgt gcggaatagc gacgcacggt ggttcggtag gcagccccgt tgggtacgtc
    13921 gcctcacgtt tcagtacgtg atcggcaaaa tcagggacga ccagctcggc ggcaaccaac
    13981 ggttgcgcta actggcggat gatctgttca acgcttaacc ctgccggaag tcgttgaata
    14041 taattttcct gaaaatgtat gccttgcatt ttattcctct taaaaaagtg tacttaactc
    14101 acttcccgcc gttgcagtac ggtgccaaga taatatttat ttccttttaa ccagacgttg
    14161 ccaaactcaa tgggtaatcc tcgggagaaa aagactaact gctccagatg caaaacgggt
    14221 gcgtcttcac tgatatctaa aaaatgacca cgttcattac caattaatcg cgcggcatag
    14281 cgactttccg agtaacgaat ttttctttgc gacaaacttt ctattgttgg aaataaattg
    14341 tgttgattaa aatcgatttc agcaataccg gggcatagct caatattgat acggttctct
    14401 atcagcatcg ctttttcatc accaattgag cgtaagcgtt caaggtaaag aatatcctga
    14461 ccgggcgtta ttcttaattt ctctgccaca taacgattag ccgattcaat ccgtgacgta
    14521 ataacttcag tggtaaaatg tattttctgg ctttccagcg attccgcgaa tgacaataat
    14581 ccttcaccta acggatatgc cacgttctct tttttgacaa atgttccctt accctggacc
    14641 tgtattaaca cgccttcttt taccagttgg gcgaccgctt ttttaacggt tccccgactc
    14701 acgtccagca tatccataat ctggtattcc gaaggaatgc gatcgtcttc caccagatct
    14761 ccggtataaa tactttctct tatccactcc accacttgtt gatagagcgg tatttgtgac
    14821 gacttactta atgatttcat ggcgcgcacc tgaacattta cagttgtcta tggacaatca
    14881 tggacaactt caagcgtata gtaggtgaac gggagaaaaa gaggatcgaa gcgtctcaca
    14941 ttttgaggga ccaaatcgtg atcaaaaaaa tgtgacgtga gtcgatattt gtcagaagtt
    15001 tactgcgccg taaggaaata acggcgcgtt ggtaggtgtt aatcttcctt cggcaggcac
    15061 tgcaaatgcc cgtgccgcac gccgcgctgg gcgataacgt catcggcaaa atgctgcacg
    15121 tcacccatgt cacctttcaa cacggcgatt tccagacagt cgtcgtggtt gatgtgcaca
    15181 tgtagcgtgg cgacggagag gtcgtggtga tgatgctggg tggaaacaat acggctggct
    15241 aagtcgcgtt tttcgtgttc atacacatac gacagcaccg cgaaaccttg tgtgccgtgc
    15301 tgctgggtag cttcctgcgc cagggcgcta cgcagaatat cgcggatagc ttcggaacgg
    15361 ttgttataac cacgacgctg gctcaggctg tccagcgtct ccagtaaatc gtcatcaagc
    15421 gtgatggtga ctcgttgcat ttgcgttaaa ccttttctgt ggcgcgacgg cgcacgggga
    15481 atgcgggtaa tatcgcgttt tgtagcacac gtccggcgtc agaggaaaag gttaatttct
    15541 ctcccaccgc ctgggtttcg acgatttgtc cgttgtccat caccatcact cgctggcaaa
    15601 agcgttccac caggcgtaag tcatgagtga tgaacaggca ggcagtgcca aactgttgtt
    15661 gtagcttttt cagcaggcga atgacgcccg cctgtaacac gagatcaagg ttagaaacgg
    15721 cttcatccag aatcagcaat ttcggttcga ccgccagcgc gcgtgccagg cagacgcgct
    15781 ggagctggcc gccgcttaac tgcggtgggc gcttgtcgag cacgctgtcg tcgagatcga
    15841 ccgcgtgcag catttcgctg gcacgcgcca gttgttcgga ttttttcagc gatagcaggt
    15901 ggcgcatcgg ttcccgcagg atctcgcgca cggttttgcg cggattcacg gcgctgatgg
    15961 agtcctgaaa taccatctga atatcgcggc ggaacgcttt acgctgggcg cgattgagct
    16021 ttgccaacga ttcgccacgc cagctaatat ttccctgtga gggcgattct aagcctacca
    16081 gtagccgcga gagagtgctt tttccgcagc cgctgcgtcc cagcagggcg acggtttcac
    16141 cgcttttcag ggtcagggaa acgttattca gcaccgcctg gtgctgatgt tttccgctaa
    16201 acccaccgtg ggcatagtga tgggaaaggt cggagacgtt aagtaaagtc atgatgccag
    16261 ctccataccg tagagggcga gatgagcgga aaccaggctg cgcgttaccg catgtttggg
    16321 ggcgttaaac agcgtttcga catcgccctg ttcgacaatc ttaccgtcag acattaccgc
    16381 tacgtcatcc gccagacgcg ccaccacgcc catatcatgg gtgaccagca gcatccccgg
    16441 cgcctgtttt tgcataatgc tttccagcag atcgagaatg cgtgcctgtg ctaccacgtc
    16501 gaggtcggtg gttggttcat cggcgatgat aaacggtgat tcgcaaagca ccgccatcgc
    16561 aatcatcatg cgctgcaaca tgccgccgct catctcgaac gggtacagct tcagcacgcg
    16621 tgcggcgttt tccagcccca ccgcttctat ggcagcggta agcgtagcgt catcggcggg
    16681 tttccccagc gccaggcacg tctcccgcgc gtgggtgtgc atggtgtgca gtggattaaa
    16741 ggcgctgcgc gggttctgca tgatggtggc aattttgatg ccgcgcaggg cgcagggaga
    16801 aaccggtttg ccatcggcta aaatttcccc cgccgtctgg cgaacgcctg cgggcagaat
    16861 tcccagcgtc gcggcgcagg ttaatgattt cccgctgccg ctaccgccga ctaacgccag
    16921 cacgcgcccg cgtttcaggg ttaacgacac gccgtgcacc agcggctgcg cggcctgtag
    16981 cgcgatatta cgtagttcaa tctgttgcgg cattagtgtg cgtgctccgt caccagatga
    17041 ggatccagat gatcgcgcag tgcgtcaccc accaggttaa aggccatcac gctgataaac
    17101 agcgccagcc ccggccagaa catttgcagc ggctgggtcc agatatactg gcgcgcgtcg
    17161 ttaatcatca cgccccattc ggcggtcggc gcggtcacgc cgaggccgag gaaggacatc
    17221 ccggcaacgt gcagcatcat atggccgata tccagcgttg ccagcaccag cagcgaaggg
    17281 atcaccgcac ctgccagatg atcaacaaac actcgcacat ggcccgcgcc agaaagccgt
    17341 gatgccagca caaactcgcg ctggcgcagt gaaatcacca ggctgcgcac catgcgtgca
    17401 taccacgccc agtgcgacag ggcgatggcg ataattacgt tggtcagccc agtgccgagc
    17461 acgccaacca taaagaatga cagaatcgag gtcgggaagg tcataaacat atcggcgacg
    17521 cgcatggtag cctgatcaac gcgcccgcca atcagcccgg cgctgccgcc aataaccagc
    17581 cctaatgtca gcaccagcag caggcaggcc attaccgaac cgagcgacac acgggtcgct
    17641 gccatcagcc gcgagaaaat atcgcgccct aagtgatcgg tgcccaacca gtgctgcgca
    17701 tccggcgaaa gcaggcgcga cggcaaatca atcgcctggg ggtcatacgg cagccaccac
    17761 tggctggtga gcgcaatcag cgccagcagg gcgataatga tcagcgccag gcgtaccgac
    17821 cagcgggaag agaggaaaaa gttcacgcgt gcgctccttc atgacgacga atgcgtgggt
    17881 ccagtgcggc gttgagcaaa tcgacaatca aattacagac cacaaaaacc accaccatca
    17941 tcagcgtaaa gcactggatc accggatagt cacggttaaa aatcgccgac accgcatagc
    18001 gcccgacgcc gggccaggta aagatgtttt cgataatcat cgtcccgcca atcagttcgc
    18061 caatgtgcat ccccacggcg gtgatcatcg gcagcgaggc attgcgcaga atatgacgac
    18121 gctcggtctg tttgtcattc aggccgcgta gccgcgccca ggtgacgtga cgctgaccgg
    18181 cgacgtccag catactggcg cgcagcagcc gtgcgttaat cgccagcgac ataaaggcaa
    18241 tggaaaccgc aggcaaaatg atgtgctgcc agccgccgta gcccatcgcg ggtagccatt
    18301 gcagatacac cgaaaacgcc atcaccagta aaaatgccag ccagaagttg ggcatcgaca
    18361 cgccgagaaa cgcgatgaaa cgtacggcga aatccggcag acggtcacga tggcgcgccg
    18421 cccagatacc gagcggtacg gaagtgagca gaattaatac cagcgccgca cccgccagtt
    18481 ccagcgtggc gggcaggaag ttcagcatat cgtccagcac cgggcgctgc gtggcaaatg
    18541 agatgccgaa atcaagatgc agcgccttcc acaaccaggt gccgtactgc acatacagcg
    18601 gctgatccag tcccagcatg gtgcgggtag aggccagcat ctccggcgtc ggcggcaggt
    18661 tagacagacg caaataatcg agcgccgggt cgccggtacc gaggcgcagc atcagaaaaa
    18721 tgatcaccga ggcggcgagc accatcggga tcagcagcag aaagcggcgt aatacgtaac
    18781 gcaacattaa ggtttcaccg gtttaatctg ttcgaacgga atttcggtgg cgatcggcgc
    18841 gtaggggatg ttacccagct ccggttttga taccaccatc attgagatgt aactgatagg
    18901 cagataaacc gcctcgtcat gcagacgggt cagaatgtcg cgatacagtg cctgacgttg
    18961 tgcttcgtca tgggtcgcca gcacttcgcc gatctcttta tcaatcagcg gcttgtcggc
    19021 taatccttgc tgtgcctgga agtcagcgtg cgacggtacg cgcattgaac tcaggaaggc
    19081 gtgtggatcg tatggcgcgc cccaggtgcg gtggaaaatc atgccaaaac gaccgtcgcg
    19141 ctgacgcgca tagatactgc tctcttcttc gccaatcagc gagacatctg cgccaatctg
    19201 gcgcatatca gcctgaatga tttccgccat cgatttgctt aacgcatcgg tgccgatgaa
    19261 cgaaagttca atgtgcagcg gctgaccatt tttctcgcgg atgtctttgc ccgcaggcag
    19321 cgtccaaccg gctttttcca gcaacgcttt cgctttctgc ggatcgtact ggcgcggttt
    19381 caggccgagg ttggcgtagg gcacagaagg ggcaaacagg gtgtcggcga cctgctgggt
    19441 gccatacaac gcgttatcaa tcagcgattt tttgtttacc gcgtaattaa gagcttcacg
    19501 tactgccagc tcattggtgg gggctttggc ggtattaagc gccagcatca cggtttcgat
    19561 cggctgtgac agttgggtat ggtaagccgg gttctggcta aagcgggcga aggtatcgag
    19621 cggtaataac ccttcgtttc cgtacagcag gtcgatatcg ccagtttcaa acgccaccgc
    19681 gcgggtagtc gggtctggga tgacgttaaa agtgatcttc ttaattgccg gcttttcgcc
    19741 ccagtagttt tcgttacgga cgaagacatc gtactgattc agtttcgatt cctgcaaaac
    19801 ccacggtccg gtgccaatcg gcgctttaat cccgttcatg gtttcatggt ttttaaactg
    19861 cgagggggcg ataaagcgga aagggcgcgg cagggccagt tcttgcagga aaggatagta
    19921 ggcgcttttc agggtgattt gcagctctgt tttactgagt gctttaacat caacaatctg
    19981 gtttgccagc tccagccagg cgtgacgttg acggttatcg agcactgcgc ggaagttttc
    20041 tgccgccgcc tctgcatcaa acggttcgcc gttagagaat ttcacgtcat cacgcagggt
    20101 gaaggtccag gttttaccat cttctgaatg agtccagctt tttgccagcc acgggatcac
    20161 cgaaccgtct gcctgatatt tcaccaatgg ttcataaacc atgctctggg cgaacatctg
    20221 gttaggcgtg taaaggtgcg ggtttagtgg cccgacattc accggccagg cggtggtgat
    20281 ttcatctggt gcagcggcat ggacgataaa agacgcacaa gccagcagcg caaatagagt
    20341 gcggcggagt gtggagagca tggtaacccc aatggattaa aatagatggc ggaaataagt
    20401 atgacgattt taagtattcg tcatactgat tacctgttct ggatcaatag atgggcagtc
    20461 gggtgggcgg atcagttaac tgaatcgatc cattgcacac tgtcggcggt gagcgtaaag
    20521 ggagtagggg tgcagatcgc caggcttaaa ttttctagct gacaatggct gactgacagc
    20581 gaggagtgat aggtgctgtc tacgctgacg atttgccagg cgctgccacc acgctgttta
    20641 acgatggctt ctttgcgcgt ccagatgcgc cagaacattt ccagttgctg atcaggatgc
    20701 acggcgtcca tctcagcatg ttccccgagg ctgaacacgg cgttcgccag ccagcgccag
    20761 ttggcgcgcg ggcgaatcac ttcgatatcg cagccgactt cgccttcatc actcaacagc
    20821 agggcgatat cgtcaccgct atggcttaag ttgaaccata gcggcgtttc cggcgcaaat
    20881 gcaggtttgc cttgttcgcc atagatgatc tccggtagcg gggaaagcgt gtgcgaaagc
    20941 aatgcacgcc ccgccagcca gcgttcgcgt cgtggacctt gcggtgcttg ctcgcgtaaa
    21001 cccggtggca gtggagctgc gcttaaggtc gaaactttcc ccagaactat ccgatacata
    21061 tcagggccaa cgtttaatga tgatggatgt attaatgccg ccaaaggcaa agttgttact
    21121 ctgcaaaaac tcacaatcaa ccttgcgggc ttcatgcatg atgtaatcca gcgcgccaca
    21181 attcgggtct ggtttattca ggtttagcgt aggcgcaaac caaccttcgc gcatcatttg
    21241 cagactcatc caggcttcga gcgcgccaca ggcaccaagc gtatgaccaa aataactttt
    21301 cagcgaggag agcggcacat tatcaccgta aatggttgct gtcgccagac tttctgccat
    21361 atcaccgcga tcggtcgccg taccatgcgc tgaaatatag ccgatatctt gtgcgcttaa
    21421 cccggcgatt ttcagcgatt gctccataca atactgcatg gtttcgcgct gcggctgggt
    21481 aatatgtgcc gcgtcgcagt tagtcgcaaa gccgacgatt tcaccgtaaa tggtcgcgcc
    21541 gcgcgctttg gcgtgctcca gttcttcgag aatgagcgta cctgcgcctt caccaatcac
    21601 cagcccgtcg cggttttcat cgaacggtga aggtgtggtt ttcggcgcat cattgtgctg
    21661 gctggtggcg aaaagcgtat caaataccgc tgcttctgaa ggacataact cttcggcacc
    21721 accagcaacc atcaccgttt gataaccgtg gcgaatggct tcccacgcat aaccgattgc
    21781 ctggctgccg gaagtacagg cgctggaggt tgggatcacc cgcccgcgca ggccgaaaaa
    21841 gaggccagta ttaacggcgg tggtgtgcgg catcatctgc acataggtgg tgccagtgat
    21901 gttattggtg tgcttttccg tcagcatggt ggcgaactcg cttaccgggc cggtactgcc
    21961 ggtcgatgaa ccgtacgcaa tgcccgtttc gccgctggtt aatatcggat cgccaatcaa
    22021 ccctgcctgc tctaacgcca gctcgctggc acgggtcgac atttgcgaca cgcggcccat
    22081 cgcacggata cgcttgcggg tgtagtgctc cggtagcgtg aaatcatcaa caggcgcgcc
    22141 aagcagggtg tgcagcccat cgtagacctg ccactccggc attttgcgca ccgcattttc
    22201 atatgccagt aagcgtgcag aaacgtcctg ccagttttca ccaaaggcag tcacgccgcc
    22261 cattcctgta atcactacgc gacgtgtcat aacatccctc cgttgatgga aataacctgg
    22321 cgggtgacgt aacccgcaat atcagacatt aaatagctgg caagcccggc aacttcttcc
    22381 gcctggccca tgcgtttcat ggggatcatc gacattgcct ctttcagggc gctctcttcc
    22441 atttcgatca tcccggtatc aataagcccc ggcgcgatgc agttgacggt gattttgcgc
    22501 ttcgcgagtt cgatagccag cgcttttgtt gcgccgataa ttccggcttt ggcagcgctg
    22561 taattcacct ggccgcgatt gcccattacg ccggaaaccg aggatagcgt gatgatgcgc
    22621 ccaccctggc gtgcgccgat catcggcata atgcacggct gaatgacgtt gtagaaactg
    22681 tcgaggttgg tgtggatcac cgcatcccag tcgtcatcgc ttaaagccgg aaaagcggca
    22741 tcgcgggcga tcccggcgtt actgaccacg ccgtaccagg caccgtgttg cgcgatttcg
    22801 tgctccagca cttcccgaca ctgttcgcgg tttgcgacgt cgaaactgag caaacgcccg
    22861 ttacccccat tggcgacaat ggcattcagc gtttcctgag cgcctgtggc gtcacggtga
    22921 tagtgaacgc caatgttaaa gccgtctgcc gccagttggc aggcgatggc gcgaccaatg
    22981 cctttgctgg caccagtaac cagaactgaa cgactcatca ggacgctcct tgttgaaaca
    23041 gggtggtaag ttcttccgcg gtcggctgga aggtgttaat gcggcccgtc gcctcgccgt
    23101 cgttgatgct gcactcaaaa ctgccgaagc gatcgtcttg catcagcagt ttgacggtaa
    23161 tggtcagtgt ctggcctgcg ggcagaatgc cagtggtgca aagcaattca cgcgcgccca
    23221 gtatcatccc taactcaata ctgtttttgc cctgctggtg gcgatgccag ccagaccaga
    23281 cgccgacggt ttgcgccatc agttcaaggg taaaccagcc ggggagattg ccgtccgggt
    23341 cgagaaacgg tgccagtacg ccactgggcg aaaccgtcac gcggcacacc gcgctgtcat
    23401 cgctgacgct caccacgtct tccagcagta acatgggcgc gtcgtggggc agataagcgc
    23461 cgggggataa atagtggctc acgaaaccct cccgagcaga atgctggcgt tattgccgcc
    23521 aaaggcgaag gaattggaca ggatcaccgg acgtgccagc ggctgcggtt tttcgataat
    23581 gccacacggt ggcagcgtgg gatcgcgcgg cgacaggctg aagtcctgag cgggcagcgg
    23641 taaatcacgc tgtaaaatca acatactaat tgccgcctcg gtaatgcccg ctgtgcccag
    23701 cgtgtgtccg gtcaaatgtt tggtggagct gcacggtacg cgttcgccaa acagggcgtt
    23761 aaccaccatt gattcaatct gatcgttgag ttgtgtggcg gtgccgtgca ggttgatata
    23821 gccgacgtca tctggcgtaa gctgcgcatc ggtcagcgcc tgattaatag cgcggatcgc
    23881 tccttcgccc tgcggatgcg gggctgaaat atggtaagcg tcgctcgatt caccaacgcc
    23941 cagcaacgcg atgggctgcg gttcccgggt cagcagcatt agccccgccc cttcaccaat
    24001 ggtgatacct gcgcggtcac gaccaaacgg ctgacataag gttggcgaca gcgattcgag
    24061 gctgtgaaaa ccgttaatcg gcatccggct cagggtgtct gcgccaccca caatggcggc
    24121 atcgaccagc cccgcttcga taagcctgcg cccgctgatt atcgctctgg cgctggaaga
    24181 gcaggcggtt gaaagggtat aggccgggcc gtcgagcgcc agccagtggc tgagaaaacg
    24241 cgacggatcg ccgagttcct gttgcgcgta gtgccatgtg gtgctcggct gcccgtgggt
    24301 gcgcaggtta acgtgcgtgt cgccttcatg caggccggag gtgctggtac caagcacaat
    24361 cgcaatgcgc tcgcggccat atttcgcaat ggcgtcatca acctgcggct ggatctgcgc
    24421 cagcgccgcc agcaggatct gattgttgcg cgagcggtgc gcagcgaatt tttccgggat
    24481 aagcggaagc tcgccatcca cgccagccag gaccgcctgc ggatgcccct gcaaccagcc
    24541 tgcgcgcggg cgcatgccgg gagcaacacc gcgagtcagg ttggttgcga tttcgtcaag
    24601 gttatttccc agggcgttga tcatgccgac ggcggaaata taaatcatat cagtcaccta
    24661 agtattgaat ggtgatgtgg tatttaaaga catgctgctc aatgctgatt ggcacgcgtt
    24721 tgcccttgcg attcagatag gtgatttccg tgaccagttt gccgctggcg ttacgcagct
    24781 cgcgtttgtc accgttgtcg cgaagtgtcc atcctgcggg aagttgcggt tgccaggcgc
    24841 taatcggcca gtggctgagc atcacgtcag ccagtacctg acttgcgggc ggtaactgtg
    24901 ggacgacgat ggattgctcg gcgcgtagcc cttgcgcatc gtaggtcacc agaaacaggc
    24961 gaatgccgac cgacgacagc ccggcaaggg tgattttctg atcgtcggcg ttaagcatca
    25021 ctaacagcga ctgggttttg ccgttgaagc tgccggtcaa cagttgctgc gaattgactg
    25081 cgggtgaaat ccctggtgcc ggcagcgtga tgcgcgtgcc aggttgcagc cacgcctgcg
    25141 ggcggccttc ctgttctggt tgcgagtggc tacagccggt gaggataagc gtagcggtca
    25201 gggcgaacgc tcgccagaat gtggatttga tcattttttt gttctctttt tatcgggcat
    25261 agccagcggc gaaagcagga aggcggtgaa aataccgctc accagcacaa tgccaaaact
    25321 gctgatggcc tgggtggcgc tgaataccag catacccagc gtcagcaagg tggtgagcat
    25381 tgccagcgcg atcgccagta gcgaagttaa cggtgtaccg cgcggattac tgaaaaacag
    25441 cgtgtagttg ataccgatgc caagcaccag caccagcgcc agcagcgaaa agagattcac
    25501 cgcctgcccg ctcatcgcca gcaccgccag accacagccc agcgaaagca ccgaaggcac
    25561 caggctgata agccctttgc gccagccgag acgggccact gcgccgcagg caatcactgc
    25621 cagcgccacc agcaacaagc cggttaagac gtagcggtaa agtgcgaaca attcatcaaa
    25681 ggtgcttttg cgatcaaccc aggcaatgcc gcaagggtaa tatgtggcga tttcctgcat
    25741 caacgcgcta cttttaaccc cttcaaccgg caccagtacg ccgctttcgc cgttttccag
    25801 cgtcagccac agcagacgcc agccttcact ggcggggctt gccagccatt catcaacgtt
    25861 cactggcatg gcgttgagat ccgggttcac tgccgtcagc ccggcatttt gcagcgcttt
    25921 tgttactgtc ggggccgccg ttttcagcag ttgtaaatct tcctcctgcc gcgcaaggga
    25981 gttcagcgga atggtgcggt agttgctgat aagcccctct tttttcgcat actcaagtga
    26041 ggcggtatat ttctccagtc gccgcaatgt ttgctgtggc gaatcgccgt aaaccacaaa
    26101 ccatttttga tcgacgctct gcccggtcag ggcggtaatg gctttttcct gcgccagaat
    26161 atgctgcggt agcgcctgta actgcgagat atcgtcatcg acgcgtagca ttgacatccc
    26221 cgccagcgaa aacagcgcca gcgcgacggg cagacccagc gacagttttt tattacgccg
    26281 ccacgctgcc agccagcgta gcatcagcgc catcgccgga accggacgca ccggcaggcc
    26341 acggcacagc cacggatgcc agaacaggac ggtcagacag gaggcgctca acccgacggc
    26401 ggcaaaaatc gccatctggc gaatgccggg gaagggggcg agcatcataa tcagatacgc
    26461 cgccacggtg gtgagcagcg ccagtagcaa ggcattgcgt actttcgcca ggctttgcca
    26521 cggcgaaacg tcgttgccgt gaaccatccg ctcggtgaga taatagagcg tgtagtcagc
    26581 ggaaatgccg ataacgctca tgctcatcac cagcgtcatc aggtgtaatt caccgaaaat
    26641 caataaagtg gcgaccgttc ccgccagcgc gccgatgccg atggaaatca cgcaaagcag
    26701 caacgggcgt aaagagcgga acaccgccac aatcagcaat atcaccccca gcagcgtagc
    26761 cacgcccagg gtggagatat cctgcttcgc ctgttggctg gcgtaatcgc tgtaaaacac
    26821 cgtgccgcgc gagagcaact gtgcctgcgg gtaacgcgtt ttcagatcct tttccagcgt
    26881 attcagggtc gtgatcagct ggtgggtttg ctgcatatca aacgacgatc ccgccagttc
    26941 gccgtgcagc agataccagt agttgccctg gggatcctgc gtcaccagcc aaccgtccat
    27001 cagccgcaaa cgctggccgt ttttcgccat tgccagctgc gagccgcgca ttaacatcag
    27061 cggatcgttt tgcagctcct tgccgcttac gccggagaat gcggaataaa gctgggagag
    27121 gatccactgt gcctgcgctt cgccgccgtt ttgcaggcgg gcgcgggtgt tggggtcaat
    27181 caggccgttg cgatgctgcc agaaaaacgc tccccacgcc tgctggctgg cggcatccat
    27241 tggcccttta acgtcgccga gcgcagcgga tttttgcagc agcgtcagcc actcctgagc
    27301 gaccaaagga ttagcctctt taccggggct gaccagccac accagttggc ggtcaagacg
    27361 ctgcataaag ccgtcattca gcgccggagg aatatcgccc atcgtctgtt tgggtaacat
    27421 agccagcacg ctactgttca gccgtgattg cggcagcagg atcagcaacg ccaccgccat
    27481 gaccaggcag actagccccc ataacagcgc ggggcgttta ctgggcggca aaacgttggc
    27541 gttcgtcatc ggtcagttgc gctggcgtca gttgatgttg ggtaagagca atatcggtgc
    27601 gatcgccctg tttatcatta agttgaatgc tctccagata ggttttcccg gcgagatcga
    27661 tggtgttgaa aattttatcc agcggcgtgg tggtcggcgt caggcgcagc gtccagcggc
    27721 cttcgccttt gtcagcaaat tcgacgcgga agttttgttc cagcactttg cgatcggcct
    27781 ggaacaacgc gcgcagcagg tggttaaact ggaacatctg cgggttgttt tctgccgtga
    27841 tgatttgcgg cggctgaccg ttgatcacct gcaccatgcg tttatcgtcc agtaatagtt
    27901 gcatggggaa cggtgaggtt tgatcccaca acagcccctg gtcgcgggcg atcaacatct
    27961 ggccctgaga tcgcagcggc tgcggcagat ctttaatcgt ccgggtttga tcgaaatggg
    28021 cgcggatcac cggttgttcg gtaaagcgtt gctgaagatc gtccagggtc agggcgctca
    28081 caaacgggct aatcagcagc gccagcagcg gtaaaaattt catggtttga ctcccatgcg
    28141 ttcaaacaga atatcggggc tgacaaagca cagttcgcga ctttgctcgt cgaccgcgac
    28201 ctgaatggtg tatccggtgg tggcgcgttt gcccgtttcg gcatcgaaaa tctgatagcc
    28261 aatccgcaga cggttttcga actcttcgat atgcgcacgc acgcggatgc gttgctcaaa
    28321 ggtgagggcg tggcgatatt tcacccgcgc atcgaccact ggccacaaat aaccggactc
    28381 tttcatttgc cgatagccgt aattgaactg gttgagcagc gcctcgcgag ccacttcaaa
    28441 gtagcgaaaa tagttaccgt gccaggccac gcccatcata tcgacgtcgt gaaagggaat
    28501 ggtcagctcg acttctgcgg taaatcgggg atcgttcagc accctttact ccttgtcctg
    28561 aatttccggc agttgccaga aatcgaaaaa attaaaccag tcgagaggcg actggagcgc
    28621 gtaatgttcc agacgcgcgg cgtaatgatc gatagcgttt tgcagcgcct gttggcgttc
    28681 gccgcgcggc agctgcagtg ggtcggcaaa gctttcgcag tgaatatgca gcttgccgtg
    28741 ctggcgcagg gcgaaaatca gattcaccgg gcagcgcaaa atagaggcga gaataaacgg
    28801 cccctgtggg aaaggcgcag gctggcccat aaacgaactc cagcagacgc gccagtcgcc
    28861 gccgcgttgc gggttgacgg cgatgcggtc accgacaatc gccacccatt cgccgttatc
    28921 cagcttctct ttcagcagga tggcggtttc tgggccgata tctgttaccg gcatcaggtt
    28981 aatgcctgcc tgaggagcca tctcctgcat tatctgttta aagcgttggg cgttttcgct
    29041 aaacaccagc gcgttaatgg ttttgtagcc ctgaattttt gccagcgccc ggcacacttc
    29101 cacatcgcca agatgcgagg ccagcagcaa tttgccgcgc ggatcgcgga cgtcaagcgc
    29161 tgcttctgcg cctggcgcaa acagcacatc acgccctggt tgtagctcgc cgcgccagct
    29221 ggcgattttg tcgagcatgg cattaccgaa acgtagaaag tgctgatagc tgttgagttt
    29281 tgccgcctgt ggttgatgct gtcgtacacg ggcgagccag tcttgcgacg ctttgcgcgc
    29341 acgagaagca gtgagccagt agacgcccac caccgggtaa agcagcgcgc taaacgccgt
    29401 tcttcccagc agacgccaga ccagcagcat caggcgcatt ccccataatc ctttcacttc
    29461 actctgccgc gcccagtggc aggaagagcg gcgcatcagc agtgaaggaa tacgcggcaa
    29521 catgccgaaa aacagacgcg tgtgcatgag cgagatgcgg acgttatctt tcagggcatc
    29581 aaaatgcgaa agcccgtcca gtggataggt gacgcgggtc ggcacgaaat agctggtatt
    29641 tccctgccag tagaggcgca ccatcacttc ggtgtcgaaa tccatccgct tgccgatggt
    29701 ggcgtgtttt gccagttgca gcgttggcgc aaccggataa acgcgaaaac cgcacatgct
    29761 gtctttcagt tgcagggaga gcgtttcgat ccatacccag acgtgggtga cccagcgccc
    29821 gtaaagccgt gagcggggga tggagtcatc gtaaattggc tggccggaga tcagtgccgc
    29881 aggttgttgt tcagcgagag ccagcagttt agggatatct tcaatcgcgt gctgaccgtc
    29941 agcatccacc tgcaccgcat ggctgaaccc tgcgtctgcc gctgcctgta agccacgcat
    30001 caccgccgcg cctttgcctg cgttttctgc caggcgaatt aaggtcacgc caggctgttc
    30061 ggcaagcaga ctgtccagtt gctgttgtgt ggtggcatcg ctgccgtcat ccaccacaat
    30121 acagggcaga ttaaatggct taagacgcgc cagcacgccc ggcatcatcg cgccgtggtt
    30181 gtagcagggt atcaacacgc agggagaaaa gtttaccgac ataggcggat cttcccgcta
    30241 ctggcggtgt ggcgggcgtc accgtcgtga cgctgatagc taaagctcag aatctggcgt
    30301 tcttcctgcc agttaagcgc gagcgtgacg cggttttccg gcagcaacgg agactggaat
    30361 tttatgttct gaatggagtg aaaacgccag ccaggcgcga gcagattgag cgcgtagctc
    30421 atcgcccagt cgatttgcgc aactcctggg agtaatggtt gcacggcaaa atgaccgcga
    30481 aaccagaaca gatccgccct gagatgcaga acaatttcaa ggtgattcgt ttgtgcctga
    30541 tggcgctcga tttcatgggg cttcatgaaa taactcctgt aattgcgcat agacacgctt
    30601 gttcatactg tttactggca tttcatcaat aacgcgccag taacgcggaa tagcaactgg
    30661 ctccagcgtc gggcgcagta aacgccgcca ggtcatttcc tggctatgcc cgccgccgtt
    30721 ctgccattgc agatgagctt cgtcatccag caccagcaaa acgcctatgc tctgacgccc
    30781 gccgcgcgta acgggaactg ccgccgcctc gcgaatgccg tccagcgcca gtagacgttg
    30841 ttctacttcc tggagtgaaa tacgtttttc ttcaatctta acgatgcgcc cacgacgccc
    30901 catcagatgg aactgaccat cttcgctaaa ctgtaagata tcgtcgagta acatgccgtt
    30961 atcttccgct atcagcggtg aaaagaggcg aaaggcatcg ccttcggcct agaactgcat
    31021 acctgggagg ggtagccagc ggcgttgctc ttgttcgcgg tagcgccagg cgatgacgcc
    31081 agtttccgtg ctgccgtaga tttcatcagg ccagacgcgc agccagctcg cggtatgttg
    31141 tacatcctgc cacggcagct caccgccagc agagagaagc acctgaacgg gtggcggaga
    31201 aagctgggta tccaggcgct tcaaaaatgc cgggctgctg atgaagatat agcgatgctg
    31261 atgactcaac gcggcaaact gttcgacata ccagagcatg gcggcgtgca gcggtaatcc
    31321 caacgccatg ggcaggaaaa cgcgaaaagt caggccgtac aaatgctgcg gcaatacgga
    31381 accgacgaca cgacaacccg ccagacgtgc gcctaatcgt tcagccagca gttctgcttc
    31441 acgatccagc aaatgaacag gtttaatcgc ccgtttcggc tgtcccgtgg aaccggaggt
    31501 aaacagctcg ataaaagcct ctggcgctat agcggcaaag gtgaaggatt gtgtcgcgat
    31561 ttgtggagaa cttgctacca acagcaatga accttgccag ttaagttcgc tgtcgctaag
    31621 cacaccatca aaaagttcac gttgctcgtt aagctggata acacggttat gccccggaag
    31681 caccggcgtt tttccggcgt gtagcgtggc gagcagggca acgataaaca gatagctgtt
    31741 ctcaaagcac agcgcccagc gttcacccgg ctgttgctgc aagcggcaga taagttgcgc
    31801 gacatcgtga cgcaaatcgc ccagcgtcca ggtactttcg ttcagccacg caatgggtgt
    31861 gtcatcaggg cgtggcgcag tcagccagcg tgcaagcggt aaggtctgct tcatgcgcga
    31921 tctcttttca tcacctgacg gcgcagcagc cattcaccag ccattaacgt ccccatcaac
    31981 aaataagcaa tcatgccgtt ccaggcggtc cataacgaca tatcggcata aagcgcggta
    32041 aagagggcaa ttccaccgtt aatgatgaaa aagccgcacc agatttgcgt gacgtggcgg
    32101 gtatagcgca cgcccttttc cgggaggtct ggctcctgca accgcgccag ccgctcaact
    32161 atcggcattg ccgaccacag cgaaccgcca aaaaccgcca gcatgaccgc gttaaccact
    32221 accgggtaaa acagcagtag ctggtgcgtt ttcaacataa agctggcgac acataatgcg
    32281 atgcccacga cagcgacgag ttgcgtgacg gcttgtaaag gccccgcctg gcgacgggtc
    32341 tggcgcagac gcaacaaaag cagcaacgcc attagcggta acagccagtg caggccgtta
    32401 tgcgctaagc caaaccaaat cacaaacggc caggcgagca gcaataatcc agtcagcagc
    32461 ttaatcatcg gtagcgagcg aatgcccgac atcagatcag gcttcttgta gcaggcgttc
    32521 tacagcctcg acgacatcct ggactgtacg cactgcttta aactcttccg gcttgatttt
    32581 cttaccggtt ttcttttgca ggtgcacaat catgtcaacg gcgtcgatgc tgtccagttc
    32641 cagatcttcg tacaggcgcg tttcaggttt gatgtcctgc ggatcgattt caaacagctt
    32701 aaccagcagg gctgagactt cctgatagac ggtttgttgt tctgtcatga tggctttcct
    32761 caggcacgtt gcgcagcaat gaaagaggcc agagtggcga cggagaaaaa gtgctgacgc
    32821 atctcttcgc tttccgcaga gagcacgatg ccgtactcat tttttactgc cagtcccagt
    32881 tccagggcat cgatagagtc cagccctaaa ccgtcgccaa acagcggcgc gtcggtatcg
    32941 atatcatcag gcgtcaactc gtccagattg agggtgctga tgatgaggtt tttaatttcc
    33001 agataaagcg cttgcattat ttattcctga gagaggtaaa caaccttgtt gtaattgaag
    33061 caaaagatgc cggtttaact gccttgctgc cagcgccggt tcttgtgaag ttgcatcgta
    33121 aaatttattg atttcaaccc gaccatggac tgcaaccgta aaaaacggcc ggtcaggtgg
    33181 aacgtcatac catttacttt gtttatccag cagatgctcg ctacagccga ttgtcacgat
    33241 gcgtaaatcg ctggcgcagc gcacggcaat attcgccgcc ccgcgctgca gcgtcatttt
    33301 ttcaccgggc ttcgttcgtg tgccttcggg gaaaatcagg atagtgtcac cctgtgccag
    33361 tcgctgttgg cagcggggta acagagtttc agcttcgctg ttgattaaat aatctgtcgc
    33421 ccgcacgacg ccgccaagaa aaggattttt taacagcgcg cttttcacca ggcagtcagt
    33481 ttctggcatg actgacgcca gcagcacgta atcgatcagc gtgggatgat tggctacgac
    33541 gaggcaaccg cgttcctgat gtaagatttc agcgccatca atacgataat ccagtacgcc
    33601 aagccctttc gcgacagtta aaaacaggcg aaaactggcg gcaatgctgc gacgcgccaa
    33661 acgacggcgg cgggaagtat ctcacaccaa aactaacaat atgttaaacc acacgacaga
    33721 aagcagcagg ccgccgagac caaacagggc aaaacataac ccggtcatga ccagtcgcca
    33781 ggtccactcc agacgtgaca tcagcttact catctgtggc tccagcgcca ttgcacgcgc
    33841 tcgccaggca gtgaaaatgt gtctacattt tgtaaatagt gctgtaaaaa caacatgctt
    33901 tgcggcagtg ttgtttcatt gcccgcaatg gctgactgcg tttcacattg ccagtcatcg
    33961 ccagcttcaa tcaccagcgc gacggcgtac ggccaggtcg gcatttctga tggcagttgt
    34021 gggtgataaa actcgggcag gaaaccgtca aaatcgacca tcagcacgcg ctggtatccc
    34081 gcttgtagca ggctaagcac ttcacacaaa ccttgctgga aactatcgcg accagccgaa
    34141 agcgatgacg aaacgctcgg ctgtttggcc gcaatggtca ggttacccac ggaagagttg
    34201 tgtacggaga gcgcgaagtc cgtcggagaa agcgcctgtt cggttgccag tgcatgaaca
    34261 atgcggtaat tgcgctccag ttcgccatga cgactggtat acaaaacggc gtctggttga
    34321 tggcggcgta gcattgccag accgcattca acagccagtt tgctcccgga gctgaggcgg
    34381 cgggcggtca tcattggcag ttcacttaat tttgccaacg gtgctgtcgg gtcaattgcc
    34441 tgaggctgac gtgaccactc ctgccattgc agcgtttcac taagtccagg cgctcttgcc
    34501 tgccagtcga gaatgtttaa tgcaaatttc atcaacgcgc gcccgcggta aaccaaaatt
    34561 attaagtgac gattatttct tttggcatat caaaagtgta tgcccaacgc ctaaattatc
    34621 gtggcgctct gctacctgga atcctgcttt attcagatag tcgtaaaatt tctctgcact
    34681 atagaaacgg ctgttaccgt ttgccatgca ggtaaaataa agtgaggacg cgttcaggct
    34741 aaacgatgct gcttcaaacc gctgcgcatc ccagaacaac tccatgatgc aaacttttgc
    34801 gccaggcttc attacgctgg caattttgct gagtatgcta attatttgtt cgggtgaaaa
    34861 acagtctaaa aattggctca tccaccagat atcagcctcg cccggtaatg gggcatcgct
    34921 tagcatatcc accgcatgga aatctatgcg atcagaaaaa cctgcattcg cgatattttc
    34981 tttcgctagt acgatttgtt gtggcagatc taataacgta acggcgatgt tttcattgta
    35041 tttgcagcaa cgcaaagccc acttacccgt attgccgcct acatcgtaca atgttgtggg
    35101 gttgttggcg aatacataag gcagtgcggc gttaaatgcg ccatctgaat agtaatgatc
    35161 aaacgcaaac cagctttcac gagcggcgtc tggtaattgc gaaagtgccg gatatatcgt
    35221 cgaccagtcg ccaaatactt ttaacccgga aggttttcct tcattgagtg aatcggcgag
    35281 gaagaagagc ccctggtaac agacatcctg ggtgaaatcc atattcacgc gagtcatagt
    35341 gtcatgcagg aggtaatgac cgattttcgc caggtaataa attccctctt tacaggtaat
    35401 aaattccctc tttacaggta ataattcgcc cgcttaagcc catatctaac agaacgctgg
    35461 tagcatattc gttgatatgg ctatgttcgg tgatatcagc cagcgttgcg ccatgtttgc
    35521 cttgccgatc cagatatgaa agtacacccg cattacgtag gcagagtgct gtttgaaaaa
    35581 gcataggggc aaaggcaatg cgttgagctt cggtaatcgc atccagtgcg ctaagagaat
    35641 cctgttcgta catgttgggc acaacctgtt aagagggaat taaattatta tcggaccgca
    35701 acggcccgac tttaggaaga attataaatc tgcgcccgct gacaaattca gttgaatatc
    35761 tttgtcgaga ttgcggaccc agcgattgta gtttttatga atttgtccgc ctgacgcttg
    35821 taggttctgg ctattttcat aattgatatc gtagctggat gccgtgtagt taatgcgtat
    35881 ttccgctgaa tgatcgcgtg actgttgacg tgctttaatt atgccttgtc cggcttcgga
    35941 cataatccat ttgcgttcaa cgccagcttt cagaatcgct tttttcactt gttcctgggt
    36001 atgaccagcg ctgacggttg agtgaacctg atcaattggc gcagtacgcg cgcaacctgc
    36061 taaagcaccc acaacagcaa tagccgcaaa ccatttcatg taatgtctca ttagaacccc
    36121 attaaagtat tgtttttacg agtaaagcaa gttttaactg taattgctgg catcactatt
    36181 ctttatttag agaaaaaatt acttgtgaga taagtcagta agtgcacatc agagtaaaaa
    36241 tctctgaaat gactaaatta ctgccagcag ggaccaacta taagtgagtg agaaaaatga
    36301 ggcaataaaa ataacacaat gttatcagtg cattctttga gaaggattta atcgctattc
    36361 ggcgtttata tatatttgac ttaaggaatg gtttttttaa attaattttc tgaataatgt
    36421 ttcatcattt cctcaaatca aatggccact gttagataaa cggtggccat tgcctgctca
    36481 ggaggtgcta ttaacccggt aaccgacttt tcggtctgcc cggccccagt aaaatcgcca
    36541 gtttgctgcc gcctttcgtc gtttccatcc agattttaca cacgctggtt aatggcaccg
    36601 aaagtagcat ccctaccggg ccgagcagcc atccccaaat taacaatgaa agaaatacca
    36661 ccatggtgga catccccagg cgatggccca tcatccgtgg ttctaaaata ttgccgatga
    36721 ccatatggac cactaaaaac aatgcgccga ccagaataca ttcgtaaaca ccattaaaca
    36781 gcagcacctg aattattggc ggtacggcgg aaattaccgc gccgatattg ggcacgtagt
    36841 taagcaaaaa cgccagtact gcccacatca gcgcaaactg tacccccatc agctccagcc
    36901 ccagccagac gatgacaccc gtccataaac tgagtagcgt ctttaacgcc agataatgcg
    36961 aaacgccttt aagtgcgcgg tgtaatcccg cgatgtgaat ctgtgggtta ttcagcgcaa
    37021 aacgcatttt gtaagggacg tggcgcactt caaacagcat aaaaactacg gtcatcacca
    37081 gcaaaagcac gctcgccatt gccccggaaa gcccggtcat tagcgctgtg gtgaaggtaa
    37141 ccactttttc cgagtccatc cgctgcagca ttcgctccgg cgacatatgc aaattaagaa
    37201 aaggcaacat ctcctgcaat ttaaaaagtt tgcgcgtcag ctccttatta aacttcggca
    37261 gcatagagat aaattcgtta aacgatgccg ccagtacgcc gaccagcgcg gttagtgcga
    37321 tcagcatcac caccactaca atcgtaatgg caacggggcg ttgtactccc cgacgaataa
    37381 accaggtgac gagcgggttg aggacgatgg caaaaaacag cgccagtaac agctgcacaa
    37441 tgatatctgc cgctgcgtga atgcccgcga ggatcactac cagcgaggcc agcttgagca
    37501 gaatgtgcat gcccgtttta tcgggttgag gggtttccat tggggcttcc ttgtgacttt
    37561 ttgtattaag tgtagcggga gtcacgacgg caatattctg attcttactg ctgattttca
    37621 cgtcagcacg tggtaaaaat gaaacactgt tgtaaaaatg tggtgatcct catgcccgaa
    37681 cccgtagccg aacccgcgct aaacggattg cgcctgaatt tgcgcattct ctccattgtc
    37741 atgtttaact tcgccagcta cctcaccatc gggttgccgc tcgctgtatt accgggctat
    37801 gtccatgatg tgatgggctt tagcgctttc tgggcaggat tggttatcag cctgcaatat
    37861 ttcgccacct tgctgagccg tcctcatgcc ggacgttacg ccgatttgct gggccccaaa
    37921 aagattgtcg tcttcggttt atgcggctgc tttttgagcg gtctgggata tctgacggca
    37981 ggattaaccg ccagtctgcc cgtcatcagc ctgttattac tttgcctggg acgcgtcatc
    38041 cttgggattg ggcaaagttt tgccggaacg ggatcgaccc tgtggggtgt tggcgtggtt
    38101 ggctcgctgc atatcgggcg ggtgatttcg tggaacggca ttgtcactta cggggcgatg
    38161 gcgatgggtg cgccgttagg cgtcgtgttt tatcactggg gcggcttgca ggcgttagcg
    38221 ttaattatta tgggcgtggc gctggtggcc attttgttgg cgatcccgcg tccgacggta
    38281 aaagccagta aaggcaaacc gctgccgttt cgcgcggtgc ttgggcgcgt ctggctgtac
    38341 ggtatggcgc tggcactggc ttccgccgga tttggcgtca tcgccacctt tatcacgctg
    38401 ttttatgacg ttaaaggttg ggacggtgcg gctttcgcgc tgacgctgtt tagctgtgcg
    38461 tttgtcggta cgcgtttgtt attccctaac ggcattaacc gtatcggcgg cttaaacgta
    38521 gcgatgattt gctttagcgt tgagataatc ggcctgctac tggttggcgt ggcgactatg
    38581 ccgtggatgg cgaaaatcgg cgtcttactg gcgggggccg ggttttcgct ggtgttcccg
    38641 gcattgggtg tagtggcggt aaaagcggtt ccgcagcaaa atcagggggc ggcgctggca
    38701 acctacaccg tatttatgga tttatcgctt ggcgtgaccg gaccactggc tgggctggtg
    38761 atgagttggg cgggcgtacc ggtgatttat ctggcggcgg cgggactggt cgcaatcgcg
    38821 ttattactga cgtggcgatt aaaaaaacgg cctccggaac acgtccctga ggccgcctca
    38881 tcatcttaaa acttactgaa taaccagcgt attaatgatg ttttctgcgg tggtctgcgc
    38941 tttttgctga tcgtcagctg gcagagtaat ttgcatggtc agcagttgat tacccacgtt
    39001 acccagaata acggaagagt acgccgtctg gcctttcgcg gagataatac tgtctaactg
    39061 ctgcattttg tgacctttca gctcaatggc tttattggtg accacttgca gctgtggatc
    39121 gcggctacgt tgctgatctt ccagacgctt cgccagcacc gccagatctt ctttcggatc
    39181 atcgcccatg atgacgatga ctgctttctg cccggtggcg tcggaccaga catgcatgtt
    39241 attggcctgc gttcccagct taccgctctg gtcggtcata tccgctggca gcgagaaact
    39301 taacttgcca tcaagcaggt tgacgggatt cccggtagcg ttactttccg cgaccgaacc
    39361 ctgcgccgta gcgttagtgt ctttatcatc acaggccgca agccccataa ccagcaggcc
    39421 aattccgaca tatttaacca gattgcgcat tgacttcttc ctttcgataa acggccataa
    39481 cggctcattc atccatctta tcacaactct gataacgaac ctttaactcg cctgcaaagc
    39541 gttgatttcg gatttatctg ccagtctttt caacagcata ttgagtaata cgccatacat
    39601 tggcaggaag aaaacgatac tgattaacac tttgaaacag taatcgacca gcgcgatttc
    39661 catccagtgt tcagccataa aggcatccgg gctacgccag aaggcaatga agaaaaaggc
    39721 cagcgtgtcg ctgacgttac cgaacagtgt ggacgctgtc ggtgccagcc accagcggcg
    39781 actctgacgc aggcggttaa aaacgtgcac gtcgaggatc tgtcccagcg cgtaggccat
    39841 gaaactggcg gtggcgatac gggcgacaaa caggttgaag tgggcgagtg cgccgaatcc
    39901 ctgccatgaa cccatataga atagcgacga gatgacgtag gagatcagca atgccgggat
    39961 catcaccgca aagatgatac gacgggccag cggtgcgcca aaaatacgta cggtcaggtc
    40021 ggtagcgaga aagataaacg gaaagctaaa cgcgccccag gtggtatgga aaccaaaaat
    40081 ggagacggga agctgaacca gatagttact ggaggtgatc accagcagat gaaataacga
    40141 taaccagaac aacgccttat agcgttgagt ttgcgagaaa acgttcatat tgtacctttt
    40201 tgattaacca ttggggtgag ggaacccaat acgtacgaca cgtctgttac cttaacataa
    40261 taacgtgctt accctttttt cgagccgccg catgatactg ctttacgttg acaatgcaat
    40321 ggttaatttt cacgcaatcg ttaacctggt ttgcttacgg actcacaggg cgtaaactag
    40381 cgccgttttt ttatgtgatg agaagaaaat gaccgatctc ttttccagcc ctgaccacac
    40441 actcgacgca cttggcctgc gctgcccgga accagtcatg atggtgcgca aaacagtgcg
    40501 caatatgcag ccaggtgaaa cattgctgat tattgctgac gatccggcga cgacccgcga
    40561 tatccctggg ttttgtacct ttatggaaca cgaactggtt gccaaagaga cggatggact
    40621 gccttatcgt tatttgattc gtaaaggcgg ttgacggggg cagattggct tcgatgccgc
    40681 cttttcccct caccctaacc ctctccccag aggagcgagg ggaccgatcg cgctcaatgt
    40741 tgcgatcggt ttgccttatc tcctgcgcaa caatcttaac gcattcgctg tcaccagcac
    40801 cgtcgcaccc gtatctgcca gcactgccag ccatagtccg gtcatcccta acagcgtggt
    40861 gacgaggaag atccctttca gccccagcgc aatagtgatg ttctggcgga tattggcgtg
    40921 agtggcgcgt gccagttcaa tcatttgcac cagaccgcgc aggtggttat gggttaatgc
    40981 tgcgtcggcg gtttccagcg ccacgtctgt gccgctgccc attgcaatcc cgatggcggc
    41041 agctttcatc gctggcgcgt cgttaatacc gtcaccgacc atcgccagcg gcgcatgttg
    41101 attcagctcg gtcaccgctt tgactttatc ttccggcaac aggcccgctt taaactccag
    41161 ccccagctcc ccggcaattg ccgccgctgc gcgtggatta tcgccggtga ggatcacccc
    41221 tttgacgccc agcgcgttca gttcactgat ggcagttgca gcatcggcgc gcagggtatc
    41281 ctgcaatgca atgataccca gcacgtcatc attacgtact accagcacaa ccgtttgccc
    41341 ggcgctttcc agttcattaa tcaaaccagc aaatgcatca gcgggatgtt tcccggcagc
    41401 gcatatcaac acgcgctcac cgttaacctg cgcttcaatg ccagacccga ccagcgcccg
    41461 ctgtgattcg gcggcgggaa tggcgagtgc agcaacctgt gcttcgcgca cgatggcctg
    41521 cgccagtgga tgcgtcgcgc cttgctcgac cgccgccgcc agtgtcagca gttcagattc
    41581 actaataccc gttgccggat gaatcgcggt aacgcgcggt ttaccgacgg tcagcgtacc
    41641 ggttttatca aacgccacct gggtaacacg acccagctgt tccagcgccg ctccgccttt
    41701 aatcaacgcc ccacgacgcg ctgccgccgc cagcccggag gtaatcgccg caggcgttga
    41761 gataactaac gcacacgggc agccaatcag cagcagcgtc agccctttat aaatccactc
    41821 ctgccagctg gtggcaaaca gcagcggtgg caccagcgtc accagcagag cgacggccat
    41881 aatcgcgggc gtgtaaatac ggctgaaacg gtcgataaac cgctcaatgg gtgcgcgacg
    41941 ctcttcggct tcttcgatca gtttcagaat ccggtcaatg gcgctggcac ccggttctga
    42001 cagcacttcc agcgttacca gacggtctac gctggtggct cctgcaggaa ctttatcgcc
    42061 cgttgcgcgc tccaccggaa tagattcgcc ggtcagggcg ctttcatcaa aactggcaaa
    42121 cggtgagagc agtttaccgt cggcaggcaa acgtccacct gcggcgactt caatcacatc
    42181 gccagggcgc aggctgttaa tcgccacctc ttcccgctca ccgttacgca ggcgcgtggc
    42241 ggtttctggt ttcagcgcca ttaacgcgct gaccccttga cgcgcgcggc tggcggccca
    42301 gccttccagt cgttcaccaa tcaaaaacag cagcaacacc atcgcagctt cagccgttgc
    42361 gccaataaac agtgcaccaa tagcggctac gctcattaag gtttcaatgg cgaagtagct
    42421 gccggatttg atcagccgta atgcctgacg agcaatcggg tacagcccaa ccagcgtggt
    42481 cgcgataaac gccagttgcc cgaacggatg attaaattgc tccaaacccc agctgattgc
    42541 catcatcacg ataagcgtaa tcagcggcag attctctttc aggcgcgatg cttgtggctc
    42601 gtcggcggcc tgctcatcgc gcagggaata gcccgctttt tgcaccgcag attcaacttg
    42661 tgcacggatg tcattgtcgg catcaaccac cagtttttcg gtggcgaaca acacctgtac
    42721 ctgattcacg cctgcaagct ggcgcacggc attttctacc ttgcgcgcac aggcggcgca
    42781 gtccatgccg ctgactttcc agctatagcg ggtgccggag acgttttcag agagagttgg
    42841 cgtgctggaa catgcgccgt cgcagcaaca gtcgttggcg ttctgtaccg tggttagcgg
    42901 tttgaacgca gcaaattgag gggctttctt gccgtgattg tcaggagtcg acatggcatc
    42961 ctccggttaa gttttttctc attaaccgaa ggatacactc tggagtcgac tccagagtca
    43021 agttttatca gagatacagc gagcggacga tgaggaagtg accggcaaag tagcaggccg
    43081 cagcaatcgc gttgtccgca cggaagcgtc ggcgatagtg actacccagc cagacaaagt
    43141 tactgataaa cagcaacgaa gcgccgacaa acgcagagag tgccggagcg gtcggacgga
    43201 agaaccacag ttcacctgcc agccacacca tcaccagcgt catgccgata aacgtgcaga
    43261 taggccaacg atactcttcc agccgcgtcc agataatcgc cagtaacagc gcacccagaa
    43321 ccagcaacac cagtggcaga ggccagaaga aagagagcgt catctgactg gcgaaataga
    43381 tggtgtacag caggtgcgag aggaaaaacg cgccgatggc gtacatcaga cgttgacgtg
    43441 gcaacagggt tagcgcatcg cccagcagtg aggcgcacag ccctgccagc accagatagc
    43501 taatggcgtc gaacatcggc gcttgccagg ccagtaacag caggagaaga agggttaacg
    43561 gtttaaacac ccagcgttgc caggttggcc cacgatacga tgcatccaca gatagccatg
    43621 cggaaagaca gacagcgata aacgaccaaa gcatcttagt tccttgaatt cgttattttt
    43681 tcgtaagcgg aatgcctacg gtcttgcttc cagtttagtg gcgagggtgg acggatgaca
    43741 acaccataac tggcagggta tgcttatttc cgcattttcc gatgagggat aaagatgagc
    43801 aaaccacctc ttttctttat tgttatcatt ggcttaatca tcgtcgccgc atcgtttcgt
    43861 tttatgcaac agcgacggga aaaagctgat aatgatatgg caccactcca gcaaaagctg
    43921 gtgttggtga gcaacaagcg ggaaaaaccg attaacgatc gccgttcgcg ccagcaggaa
    43981 gtgactccgg caggcaccag tatgcgctat gaggcaagct tcaaaccgca aagcggagga
    44041 atggagcaga cgtttcgcct cgacgcccag cagtaccacg ccctgacagt gggcgataaa
    44101 ggtacgctga gctataaagg aacgcgcttt gttagctttg ttggcgaaca ataaggttat
    44161 tttttaactt taaatttctt ctgccagacc agcagttcaa acacgccgaa aaggaaaatc
    44221 cgcacctttt cggcggtggt catctgcggg ccatctttcg gtaaagtgga tttcaatagc
    44281 gccagttgca taccatgcat cagcacggta aaaatcagcg ccacgttaac gaaaatattc
    44341 agcgggcgcg ggaagggatg caccaggttg aggattaaaa atccccaaac acagagcatt
    44401 aacaaacgac caatattaat cagcatcact ttctccttgt gcttcgcgtt gatacagccg
    44461 ataagccacc tgacccgcca ctttttcccg atgtaacgac cagtttgccg gaacggtggg
    44521 cagaccgttt tcgacttcgc tttcgacata aatcagggtt tcgtcagcca gccagccgtt
    44581 atcttccagt aaatttatcg tctcttctaa caaaccacgg cggaacggtg gatcgacaaa
    44641 cacgatatta tgcggtgtgc ctttttgcgc caggaatgac attgcgttgc tgttcaccac
    44701 gcgtgcattg cctgctttta gtgtcgccag attcttaatt aactgctgag aaactgcacg
    44761 atccatctca attaacgtgg cccccgcagc gtagcgcgat aacgcttcca gccccagcgc
    44821 gccgctcccg gcgaagcaat ccagacattg ggcgtcaaca atgaccggag ccagccagtt
    44881 aaacaacgtt tcgcgtacgc ggtcggtggt ggggcgcaga cctgggctat caggaaccgg
    44941 gagtttacgg cctcgccact gcccgccaat aatgcggatt tggccgctgc cggaatgatt
    45001 cggttttttc atgataaatt gctcaatccg ccagataaca catacttgca ggcggtgatg
    45061 tgaattaagt taagtgatag actatttcat cattttttta gctgctatgt acatagcgtt
    45121 aacgctgtgc catgaagcaa cagcgaggag tgtagtcgca aatggcgaaa gaaaaaaaac
    45181 gtggcttttt ttcctggctg ggctttggtc aaaaagagca gaccccggaa aaagagacag
    45241 aagttcagaa tgaacaaacg gttgtagaag aaatcgttca ggcgcaagag cctgtgaagg
    45301 cttctgaaca agccgtcgaa gagcagccgc aggcgcatac tgaggccgag gcggaaactt
    45361 ttgctgccga cgttgtggaa gtcactgaac aggttgctga aaatgaaaaa gcgcagcctg
    45421 aagcggaagt cgttgcacaa ccggaaccgg tcgtagaaga aacgccggag ccagtggcta
    45481 tcgaacgtga agagctgccg ttgccggaag acgtcaacgc cgaagaggtt tcgccagaag
    45541 agtggcaggc tgaagcggaa accgtagaga ttgtcgaagc ggcggaagaa gaagcggcta
    45601 aagaagaaat caccgacgaa gagctggaag cacaggcgct ggctgccgaa gcagcagaag
    45661 aggcggtgat ggtggttcct ccggcagaag aagagcagcc ggtggaagaa atcgctcagg
    45721 agcaggaaaa accgaccaaa gaaggttttt tcgcgcgcct gaaacgcagc ctgttaaaaa
    45781 ccaaagaaaa tctcggttcc ggatttatca gcctgttccg cggtaaaaaa atcgacgatg
    45841 atctgtttga ggagctggaa gagcagcttt tgatcgccga tgtgggcgtg gaaaccacac
    45901 gtaaaattat caccaatctg acggaaggcg catcccgcaa gcagcttcgt gatgccgagg
    45961 cgctctatgg cctgctgaaa gaagagatgg gtgagattct ggcgaaagtc gatgagccgc
    46021 tgaatgttga aggtaaaacg ccgttcgtga tcctgatggt gggcgtcaac ggtgtgggta
    46081 aaaccacgac gattggtaag ctggcacgtc agtttgagca gcagggtaaa tcggtgatgc
    46141 tggcggcggg cgatactttc cgtgcagcag cggttgaaca gcttcaggtc tgggggcagc
    46201 gcaacaatat tccggtgatt gcccagcata ccggggcgga ttccgcctct gttatcttcg
    46261 acgccattca ggcggctaaa gcgcgtaata tcgacgtcct gattgccgat accgccggac
    46321 gcctgcagaa caaatcgcac ctgatggaag agttgaagaa aatcgtccgc gtgatgaaga
    46381 aactcgacgt tgaagcgccg catgaagtta tgctgactat cgatgccagc accgggcaga
    46441 acgcggtaag ccaggccaaa ctgttccatg aagccgttgg cttaaccggc atcacgctaa
    46501 cgaaactgga cggcacggcg aaaggcgggg taattttctc ggtggctgac cagtttggta
    46561 tccctatccg ctacattggt gtcggcgaac gtattgagga tttgcgtccg tttaaggcgg
    46621 acgactttat agaggcactt tttgcccgag aggattaaca atgattcgct ttgaacatgt
    46681 cagcaaggct tatctcggtg ggagacaggc gctgcagggc gttacgttcc atatgcagcc
    46741 gggtgagatg gcgtttctga ccggtcattc cggcgcaggg aaaagtaccc tcctgaagct
    46801 gatctgtggg attgagcggc ccagcgccgg gaaaatctgg tttagcggcc atgacatcac
    46861 gcgtctgaaa aaccgtgaag ttccgtttct gcgccgccag attggcatga ttttccagga
    46921 tcaccatcta ctgatggacc gtactgtcta cgataacgtg gcgatcccgc tgattatcgc
    46981 cggtgccagc ggtgacgata ttcgccgccg ggtgtcggcg gcactggata aagtcgggct
    47041 actggacaaa gcgaagaact tccctattca gctttctggc ggtgaacaac agcgtgttgg
    47101 cattgcccgc gcggtggtga acaagcccgc ggtactgctg gcggacgaac cgactggtaa
    47161 cctggacgac gcgttgtcgg aagggattct acgtctgttt gaagagttta accgcgtagg
    47221 ggtaaccgta ttgatggcaa cgcacgacat caacctgatt tcacggcgtt cctatcgcat
    47281 gctcaccctg agcgatggtc acttgcatgg aggcgtgggc catgaataag cgcgatgcaa
    47341 tcaatcatat tcggcagttt ggcgggcgtc ttgatcgctt ccgtaaatcg gtcggcggct
    47401 caggcgacgg cggtcgtaac gcaccgaaac gcgcgaaatc ctcgccaaaa ccggtaaatc
    47461 gcaaaaccaa cgttttcaac gaacaggtgc gctatgcctt ccacggcgca ttgcaggatc
    47521 tgaaaagcaa accgttcgcc acatttttaa cggtgatggt tatcgccatc tccctgacgc
    47581 tgcccagcgt ctgttatatg gtgtacaaaa acgttaacca ggcggcgacg cagtattatc
    47641 cgtcaccgca aatcactgtt tatctgcaaa aaacgctgga cgatgacgcc gctgcgggcg
    47701 tggtggcaca gttgcaggcc gagcaaggcg tagagaaagt gaactatctt tctcgtgaag
    47761 acgcactggg cgagttccgt aactggtctg gttttggtgg cgcgctggat atgctggaag
    47821 aaaacccgct tccggcagtg gcggtggtga tcccgaagct cgactttcag gggacggagt
    47881 ctctgaatac gctgcgtgat cgtatcacgc agattaacgg cattgacgaa gtgcggatgg
    47941 atgacagctg gtttgcccgt ctggcggcgt tgaccgggct ggtcgggcgc gtttcggcga
    48001 tgatcggcgt gttgatggtg gcggcagtgt tcctcgtcat cggtaacagt gtacgcctga
    48061 gtatctttgc tcgtcgtgac tccattaacg tgcagaaact gattggtgca acggatggtt
    48121 tcatcctgcg cccgttcctg tacggtggcg cattgctggg gttttctggc gcattgttgt
    48181 cattaatttt atcagaaatt ctggtactgc gattgtcatc ggcggttgcg gaagtggcac
    48241 aggttttcgg aacgaagttt gatatcaatg gtttatcgtt cgatgaatgc ctgctgttgc
    48301 tgctggtatg ctcgatgatt ggctgggtgg cagcgtggct tgccacggta caacatttac
    48361 gccactttac gcctgaataa taaaagcgtg ttatactctt tccctgcaat gggttccgta
    48421 gcagggaaag agaccccgtt gtctcttccc ggtatttcat ctctatgtca cattttgtgc
    48481 gtaatttatt cacaagcctg cattgaactt gtggataaaa tcacggtctg ataaaacagt
    48541 gaatgataac ctcgttgctc ttaagctctg gcacagttgt tgctaccact gaagcgccag
    48601 aagatatcga ttgagaggat ttgaatgact gacaaaatgc aaagtttagc tttagcccca
    48661 gttggcaacc tggattccta catccgggca gctaacgcgt ggccgatgtt gtcggctgac
    48721 gaggagcggg cgctggctga aaagctgcat taccatggcg atctggaagc agctaaaacg
    48781 ctgatcctgt ctcacctgcg gtttgttgtt catattgctc gtaattatgc gggctatggc
    48841 ctgccacagg cggatttgat tcaggaaggt aacatcggcc tgatgaaagc agtgcgccgt
    48901 tttaacccgg aagtgggtgt gcgcctggtc tccttcgccg tccactggat caaagcagag
    48961 atccacgaat acgttctgcg taactggcgt atcgtcaaag ttgcgaccac caaagcgcag
    49021 cgcaaactgt tcttcaacct gcgtaaaacc aagcagcgtc tgggctggtt taaccaggat
    49081 gaagtcgaaa tggtggcccg tgaactgggc gtaaccagca aagacgtacg tgagatggaa
    49141 tcacgtatgg cggcacagga catgaccttt gacctgtctt ccgacgacga ttccgacagc
    49201 caaccgatgg caccggtgct ctatctgcag gataaatcat ctaactttgc cgacggcatt
    49261 gaagatgata actgggaaga gcaggcagca aaccgtctga ccgacgcgat gcagggtctg
    49321 gacgaacgca gccaggacat catccgtgcg cgctggctgg acgaagacaa caagtccacg
    49381 ttgcaggaac tggctgaccg ttacggtgtt tccgctgagc gtgtgcgcca gctggaaaag
    49441 aacgcgatga aaaaactgcg cgctgctata gaagcgtaat ttccgctatt aagcagagaa
    49501 ccctggatgc gagtccgggg tttttgtttt ttgggcctct acaataatca atttcccctc
    49561 cggcaaaacg ccaatcccca cgcagattgt taataaactg tcaaaatagc tattccaata
    49621 tcataaaaat cgggtatgtt ttagcagagt atgctgctaa agcacgggta gtcatgcata
    49681 aaacgaaata aagtgctgaa caacaacatc acaacacacg taataaccag aagaatgggg
    49741 attctcagga tgaacataaa gggtaaagcg ttactggcag gatgtatcgc gctggcattc
    49801 agcaatatgg ctctggcaga agatattaaa gtcgcggtcg tgggcgcaat gtccggtccg
    49861 gttgcgcagt acggtgacca ggagtttacc ggcgcagagc aggcggttgc ggatatcaac
    49921 gctaaaggcg gcattaaagg caacaaactg caaatcgtaa aatatgacga tgcctgtgac
    49981 ccgaaacagg cggttgcggt ggcgaacaaa gtcgttaacg acggcattaa atacgtgatt
    50041 ggtcacctct gttcttcatc aacgcagcct gcgtctgaca tctacgaaga cgaaggcatt
    50101 ttaatgatca ccccagcggc aaccgcgccg gagctgaccg cccgtggcta tcagctgatc
    50161 ctgcgcacca ccggcctgga ctccgaccag gggccgacgg cggcgaaata tattcttgag
    50221 aaagtgaaac cgcagcgtat tgctatcgtt cacgacaaac agcaatacgg cgaaggtctg
    50281 gcacgagcgg tgcaggacgg cctgaagaaa ggcaatgcta acgtggtgtt ctttgatggt
    50341 atcaccgccg gggaaaaaga tttctcaacg ctggtggcgc gtctgaaaaa agagaatatc
    50401 gacttcgttt actacggcgg ttatcacccg gaaatggggc aaatcctgcg tcaggcacgc
    50461 gcggcagggt tgaaaactca gtttatgggg ccggaaggcg tggctaacgt ctcgctgtct
    50521 aacattgcgg gcgaatcagc ggaagggctg ctggtgacca agccgaagaa ctacgatcag
    50581 gttccggcga acaaacccat tgttgatgcg atcaaagcga aaaaacagga cccaagcggc
    50641 gcattcgttt ggaccaccta cgccgcgctg caatctttgc aggcgggcct gaatcagtct
    50701 gacgatccgg ctgaaatcgc caaatacctg aaagcgaact ccgtggatac cgtaatggga
    50761 ccgctgacct gggatgagaa aggcgatctg aaaggctttg agttcggtgt gtttgactgg
    50821 cacgccaacg gcacggcaac cgatgcgaag taacagatag ataaatgaaa atgcccgatg
    50881 cgctgaactg ccatcgggtt tttttgtgtt tgtcgaaatg gttgtagaaa gattaaacaa
    50941 tccaggatat gcttaatgta cggtgacata ccgtacatat tatacggagc aggagagttc
    51001 acatgtctgc tgttaaaaag cagcgtatcg atctgcgttt aactgacgat gacaaaagta
    51061 tgattgagga agcggcagcg atatctaatc agtccgtcag ccaatttatg ttgaacagcg
    51121 cttcgcagcg ggctgcggaa gtgattgaac agcatcggcg ggtgatcctc aatgaggaat
    51181 cctggacgcg ggtgatggat gcgctgagta atccaccgtc accaggtgaa aagctaaaac
    51241 gtgcggcaaa acgtcttcag ggaatgtaat aatttatgga tgatctgacg atagagattc
    51301 tgaccgatga tgcagattat gatctacagc gattcgactg cggcgaggaa gcgttaaatc
    51361 tctttctgac gacacatctc gttcgtcaac atcgcaacaa aattctgcga gcgtatatcc
    51421 tttgtcgcaa cactccagaa cgtcaggtgc tgggatatta cacattatgc ggcagttgtt
    51481 ttgaacgagc cgcattgccc tcgaaatcga aacagaaaaa aattccctac aaaaatattc
    51541 ccagcgttac tcttgggcgt ctggcaattg atcgttcatt acaggggcag ggatggggag
    51601 caacactggt tgctcatgcc atgaaagtcg tctggtcagc ctctttagcg gtaggtattc
    51661 acggtctttt tgtcgaggcg ctgaatgaaa aagcccatac gttttataaa tcgctgggct
    51721 ttatcccttt agtcggggaa aacgaaaatg cgttattttt cccaaccaaa tccattgaac
    51781 tgctttttac acagagcgat taacgcttct cccagccgcc ctgctgtgcc gtaaatccca
    51841 gtgcctgcat aaacgccgtc atcacaccgc gatcttccac gcctgcatcc gccatccacc
    51901 agcatgaaac gccaggattg ttacgcaaaa cctcttccag cagatattgc cccacaccgc
    51961 gacgtcgagt gacttcccgc acgcgcaggg aatccagtgc cccctcggtg ccgcttaagg
    52021 ttacccgcac ggcagcaagc aggcgctcgt taaaacgcgc ggcgtagatg cggtggttat
    52081 cgtcaacctg taaagaggaa ggggaatact ccggccagat cttttgcagg tcaatccggt
    52141 cctggtcgct aaatttttct aatcgaatga tggtcagctt catgggtaac ccgtgtaaat
    52201 cacaaaagtg gaaccagtgt agcgaaataa tttaatcgga ggctttctct tttttatttc
    52261 ttttggcagg tgattaattt cttaacagca ataattacaa agttaaaaca ttagagaatg
    52321 aaaaat
//
LOCUS       DABSYT020000021        51244 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.021, whole genome shotgun sequence.
ACCESSION   DABSYT020000021 DABSYT020000000
VERSION     DABSYT020000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 51244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.021"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            92..616
                     /gene="lpfA1"
                     /locus_tag="ID725_RS08265"
                     /old_locus_tag="pgaptmp_001653"
     CDS             92..616
                     /gene="lpfA1"
                     /locus_tag="ID725_RS08265"
                     /old_locus_tag="pgaptmp_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HGV7850279.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            681..1367
                     /gene="lpfB"
                     /locus_tag="ID725_RS08270"
                     /old_locus_tag="pgaptmp_001654"
     CDS             681..1367
                     /gene="lpfB"
                     /locus_tag="ID725_RS08270"
                     /old_locus_tag="pgaptmp_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HGV7850280.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSVSKIKYNTINDFGS
                     SAEMITKNVD"
     gene            1396..3969
                     /gene="lpfC"
                     /locus_tag="ID725_RS08275"
                     /old_locus_tag="pgaptmp_001655"
                     /pseudo
     CDS             1396..3969
                     /gene="lpfC"
                     /locus_tag="ID725_RS08275"
                     /old_locus_tag="pgaptmp_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            3984..5039
                     /locus_tag="ID725_RS08280"
                     /old_locus_tag="pgaptmp_001656"
     CDS             3984..5039
                     /locus_tag="ID725_RS08280"
                     /old_locus_tag="pgaptmp_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7850281.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            5044..5574
                     /gene="lpfE"
                     /locus_tag="ID725_RS08285"
                     /old_locus_tag="pgaptmp_001657"
     CDS             5044..5574
                     /gene="lpfE"
                     /locus_tag="ID725_RS08285"
                     /old_locus_tag="pgaptmp_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HGV7850282.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5829..7520
                     /gene="eptB"
                     /locus_tag="ID725_RS08290"
                     /old_locus_tag="pgaptmp_001658"
     CDS             5829..7520
                     /gene="eptB"
                     /locus_tag="ID725_RS08290"
                     /old_locus_tag="pgaptmp_001658"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGV7850283.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7612..7688
                     /locus_tag="ID725_RS08295"
                     /old_locus_tag="pgaptmp_001659"
     tRNA            7612..7688
                     /locus_tag="ID725_RS08295"
                     /old_locus_tag="pgaptmp_001659"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7646..7648,aa:Pro,seq:cgg)
     gene            8433..10040
                     /gene="dppA"
                     /locus_tag="ID725_RS08300"
                     /old_locus_tag="pgaptmp_001660"
     CDS             8433..10040
                     /gene="dppA"
                     /locus_tag="ID725_RS08300"
                     /old_locus_tag="pgaptmp_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGV7850284.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10191..11210
                     /gene="dppB"
                     /locus_tag="ID725_RS08305"
                     /old_locus_tag="pgaptmp_001661"
     CDS             10191..11210
                     /gene="dppB"
                     /locus_tag="ID725_RS08305"
                     /old_locus_tag="pgaptmp_001661"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGV7850285.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11220..12122
                     /gene="dppC"
                     /locus_tag="ID725_RS08310"
                     /old_locus_tag="pgaptmp_001662"
     CDS             11220..12122
                     /gene="dppC"
                     /locus_tag="ID725_RS08310"
                     /old_locus_tag="pgaptmp_001662"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGV7850286.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12133..13116
                     /gene="dppD"
                     /locus_tag="ID725_RS08315"
                     /old_locus_tag="pgaptmp_001663"
     CDS             12133..13116
                     /gene="dppD"
                     /locus_tag="ID725_RS08315"
                     /old_locus_tag="pgaptmp_001663"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGV7850287.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13113..14117
                     /gene="dppF"
                     /locus_tag="ID725_RS08320"
                     /old_locus_tag="pgaptmp_001664"
     CDS             13113..14117
                     /gene="dppF"
                     /locus_tag="ID725_RS08320"
                     /old_locus_tag="pgaptmp_001664"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGV7850288.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14147..15418)
                     /gene="yhjV"
                     /locus_tag="ID725_RS08325"
                     /old_locus_tag="pgaptmp_001665"
     CDS             complement(14147..15418)
                     /gene="yhjV"
                     /locus_tag="ID725_RS08325"
                     /old_locus_tag="pgaptmp_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGV7850289.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            15894..16001
                     /gene="ldrD"
                     /locus_tag="ID725_RS08330"
                     /old_locus_tag="pgaptmp_001666"
     CDS             15894..16001
                     /gene="ldrD"
                     /locus_tag="ID725_RS08330"
                     /old_locus_tag="pgaptmp_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGV7850290.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16088..17767)
                     /gene="bcsG"
                     /locus_tag="ID725_RS08335"
                     /old_locus_tag="pgaptmp_001667"
     CDS             complement(16088..17767)
                     /gene="bcsG"
                     /locus_tag="ID725_RS08335"
                     /old_locus_tag="pgaptmp_001667"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGV7850291.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17764..17955)
                     /gene="bcsF"
                     /locus_tag="ID725_RS08340"
                     /old_locus_tag="pgaptmp_001668"
     CDS             complement(17764..17955)
                     /gene="bcsF"
                     /locus_tag="ID725_RS08340"
                     /old_locus_tag="pgaptmp_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGV7850292.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(17952..19523)
                     /gene="bcsE"
                     /locus_tag="ID725_RS08345"
                     /old_locus_tag="pgaptmp_001669"
     CDS             complement(17952..19523)
                     /gene="bcsE"
                     /locus_tag="ID725_RS08345"
                     /old_locus_tag="pgaptmp_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGV7850293.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            19796..19984
                     /gene="bcsR"
                     /locus_tag="ID725_RS08350"
                     /old_locus_tag="pgaptmp_001670"
     CDS             19796..19984
                     /gene="bcsR"
                     /locus_tag="ID725_RS08350"
                     /old_locus_tag="pgaptmp_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGV7850294.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            19996..20748
                     /gene="bcsQ"
                     /locus_tag="ID725_RS08355"
                     /old_locus_tag="pgaptmp_001671"
                     /pseudo
     CDS             19996..20748
                     /gene="bcsQ"
                     /locus_tag="ID725_RS08355"
                     /old_locus_tag="pgaptmp_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            20745..23363
                     /gene="bcsA"
                     /locus_tag="ID725_RS08360"
                     /old_locus_tag="pgaptmp_001672"
     CDS             20745..23363
                     /gene="bcsA"
                     /locus_tag="ID725_RS08360"
                     /old_locus_tag="pgaptmp_001672"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HGV7850295.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            23374..25749
                     /gene="bcsB"
                     /locus_tag="ID725_RS08365"
                     /old_locus_tag="pgaptmp_001673"
     CDS             23374..25749
                     /gene="bcsB"
                     /locus_tag="ID725_RS08365"
                     /old_locus_tag="pgaptmp_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGV7850296.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            25756..26862
                     /gene="bcsZ"
                     /locus_tag="ID725_RS08370"
                     /old_locus_tag="pgaptmp_001674"
     CDS             25756..26862
                     /gene="bcsZ"
                     /locus_tag="ID725_RS08370"
                     /old_locus_tag="pgaptmp_001674"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGV7850297.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            26844..30317
                     /gene="bcsC"
                     /locus_tag="ID725_RS08375"
                     /old_locus_tag="pgaptmp_001675"
     CDS             26844..30317
                     /gene="bcsC"
                     /locus_tag="ID725_RS08375"
                     /old_locus_tag="pgaptmp_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGV7850298.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDLDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWALQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLRDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            30399..32387
                     /gene="hmsP"
                     /locus_tag="ID725_RS08380"
                     /old_locus_tag="pgaptmp_001676"
     CDS             30399..32387
                     /gene="hmsP"
                     /locus_tag="ID725_RS08380"
                     /old_locus_tag="pgaptmp_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGV7850299.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            32570..33856
                     /gene="dctA"
                     /locus_tag="ID725_RS08385"
                     /old_locus_tag="pgaptmp_001677"
     CDS             32570..33856
                     /gene="dctA"
                     /locus_tag="ID725_RS08385"
                     /old_locus_tag="pgaptmp_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGV7850300.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            34077..35573
                     /gene="yhjJ"
                     /locus_tag="ID725_RS08390"
                     /old_locus_tag="pgaptmp_001678"
     CDS             34077..35573
                     /gene="yhjJ"
                     /locus_tag="ID725_RS08390"
                     /old_locus_tag="pgaptmp_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGV7850301.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(35669..36598)
                     /gene="kdgK"
                     /locus_tag="ID725_RS08395"
                     /old_locus_tag="pgaptmp_001679"
     CDS             complement(35669..36598)
                     /gene="kdgK"
                     /locus_tag="ID725_RS08395"
                     /old_locus_tag="pgaptmp_001679"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGV7850302.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            36830..37597
                     /gene="pdeH"
                     /locus_tag="ID725_RS08400"
                     /old_locus_tag="pgaptmp_001680"
     CDS             36830..37597
                     /gene="pdeH"
                     /locus_tag="ID725_RS08400"
                     /old_locus_tag="pgaptmp_001680"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGV7850303.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            37667..39727
                     /gene="yhjG"
                     /locus_tag="ID725_RS08405"
                     /old_locus_tag="pgaptmp_001681"
     CDS             37667..39727
                     /gene="yhjG"
                     /locus_tag="ID725_RS08405"
                     /old_locus_tag="pgaptmp_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGV7850304.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(39909..41231)
                     /gene="yhjE"
                     /locus_tag="ID725_RS08410"
                     /old_locus_tag="pgaptmp_001682"
     CDS             complement(39909..41231)
                     /gene="yhjE"
                     /locus_tag="ID725_RS08410"
                     /old_locus_tag="pgaptmp_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850305.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(41643..42656)
                     /gene="yhjD"
                     /locus_tag="ID725_RS08415"
                     /old_locus_tag="pgaptmp_001683"
     CDS             complement(41643..42656)
                     /gene="yhjD"
                     /locus_tag="ID725_RS08415"
                     /old_locus_tag="pgaptmp_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGV7850306.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(42705..43604)
                     /gene="rcdB"
                     /locus_tag="ID725_RS08420"
                     /old_locus_tag="pgaptmp_001684"
     CDS             complement(42705..43604)
                     /gene="rcdB"
                     /locus_tag="ID725_RS08420"
                     /old_locus_tag="pgaptmp_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7850307.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            44124..44726
                     /gene="yhjB"
                     /locus_tag="ID725_RS08425"
                     /old_locus_tag="pgaptmp_001685"
     CDS             44124..44726
                     /gene="yhjB"
                     /locus_tag="ID725_RS08425"
                     /old_locus_tag="pgaptmp_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGV7850308.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(44777..46426)
                     /gene="treF"
                     /locus_tag="ID725_RS08430"
                     /old_locus_tag="pgaptmp_001686"
     CDS             complement(44777..46426)
                     /gene="treF"
                     /locus_tag="ID725_RS08430"
                     /old_locus_tag="pgaptmp_001686"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGV7850309.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            46831..48228
                     /gene="ccp"
                     /locus_tag="ID725_RS08435"
                     /old_locus_tag="pgaptmp_001687"
     CDS             46831..48228
                     /gene="ccp"
                     /locus_tag="ID725_RS08435"
                     /old_locus_tag="pgaptmp_001687"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGV7850310.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            48439..49839
                     /gene="gadA"
                     /locus_tag="ID725_RS08440"
                     /old_locus_tag="pgaptmp_001688"
     CDS             48439..49839
                     /gene="gadA"
                     /locus_tag="ID725_RS08440"
                     /old_locus_tag="pgaptmp_001688"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGV7850311.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            50210..51034
                     /gene="gadX"
                     /locus_tag="ID725_RS08445"
                     /old_locus_tag="pgaptmp_001689"
     CDS             50210..51034
                     /gene="gadX"
                     /locus_tag="ID725_RS08445"
                     /old_locus_tag="pgaptmp_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGV7850312.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
BASE COUNT        12431 a        12646 c        13812 g        12355 t
ORIGIN      
        1 cgtatttcat tgtataatcc ttccttcaaa gttgtcgatt ttaatacatc aagattttct
       61 ttttaatgta attttttaaa tggagttttt catgaaaaag gtcgtttttg ccttaaccgc
      121 actggcgtta acttctggca ctgtattcgc agcagagtct ggtgacggca cggttaaatt
      181 tacaggcgag atcgtggatt caccttgcgt actgtccgtt gactctcaga accaggaagt
      241 tgtgttgggt caggtacaaa aatctgtatt tgctgcggtt ggtgacaaat ccccggcaaa
      301 acctttcgaa atcaaactgg aagattgcga taccaccacg atgaaaaaag ccaatgtgag
      361 ctttagtggt gttggtgatg cagataaatc tgatctgatt tctgttagca ctgaagcggg
      421 cgcggccaaa ggtgttggta tcggtattta tgataacagc aataccctgg tcgctcttaa
      481 cggtggtaaa gcaagtgttg atctgagtaa aggccagacg gttctctact ttaccgctaa
      541 ctacgtttct actctggcaa cggtaacgac cggttacggc aatgcgcagg tcgatttcaa
      601 cctgtcttac gagtaatcgt ccgttttaca aatggcggcg taattgccgc cattagtcgt
      661 caaagttcat ggatcgaatg atgaaaagta aatttgttgc gttggctctc tctctgttcc
      721 tttcacagtc agttctggcg ggtggcgtcg gacttagctc tactcgcgta atttatgatg
      781 gcagtaagaa agaagcctcg ctgaccgtac agaataaaag taagacggaa gagtttctta
      841 tccaatcctg ggtggatgat gccgcgggca gcaaaaaaac accgtttatt attacgcccc
      901 cactattcaa gcttgatcca gaaaagaaca atattttgcg tattgtgaat attacgcccg
      961 gcctgcctca ggatcgcgaa tcggtctact gggttaatgt gaaagccatt ccatctaaaa
     1021 gtgacgacag cgaaaacaaa aatgttttgc agattgccgt tcgcacccga atcaaacttt
     1081 tttatcgccc ggcaggttta aagggtgatg taaaaaccgc gccaaatgaa ctgcgattta
     1141 cgcgtaacgg taatcagctc cgcgttgata atccgacagt ttttaatatt acctttaatc
     1201 aattttttgc gaatgacaaa gaaattgaaa aagcaggcat ggtacccgct aaaggggcat
     1261 taaatatcac attacccgcc ggggtaggta gcgtttctaa aattaaatat aacaccatca
     1321 atgactttgg atcgtcagcg gaaatgatca ctaaaaatgt tgattgatta atatcttttc
     1381 aggagaaata gctcaatgtc acggaagacc gtttcacgta ctttttcttc cttttctatc
     1441 tctgtggtgg cggttgctgt tgccagcacg ttttctgcac atgccggaaa gtttaacccc
     1501 aaattcctgg aggacgttca gggtgtgggc cagcatgtcg atttaacgat gttcgaaaaa
     1561 ggccaggaac agcagttacc cggcatctat cgtgtctctg tttacgtcaa tgaacagaga
     1621 atggaaaccc gcacactgga atttaaagag gccacagaag cacagcgcaa agcgatgggg
     1681 gaatcccttg tgccttgcct tagccggacg caactggcag agatgggcgt gcgcgttgaa
     1741 agctttccgg ccctcaatct ggtgtctgcc gaagcctgcg taccgtttga cgaaatcatt
     1801 cccctggcct caagccattt cgacttcagt gaacaaaagc tggtattaag tttccctcag
     1861 gcggcgatgc accaggtggc gcgcggcacg gtgccggaat ctttatggga cgaaggtatc
     1921 cctgcgttgc tgctggatta cagttttagc gggagcaaca gtgaatatga ctcgacgggg
     1981 tcttccagca gttatgtcga tgataatggc actgttcatc atgatgatgg taaagatacg
     2041 ctaaagagcg acagctacta cctcaactta cgcagcgggc tgaaccttgg cgcttggcgg
     2101 ttacggaatt acagtacctg gagccattcc ggtggcaaag cgcagtggga taatatcggt
     2161 acgtctttga gtagggccat catccctttc aaagcgcaat taacaatggg ggatacggcg
     2221 accgcggggg acatttttga cagcgttcag atgcgtggcg cgatgttagc ctctgatgaa
     2281 gagatgctgc ccgacagcca gcgaggcttt gcacccatcg ttcgagggat cgccaaaagc
     2341 aacgccgaag tcagtattga acaaaacggc tacgttattt atcgcaccta tgtgcagcct
     2401 ggtgcctttg aaataaacga tctttacccc actgcaaaca gcggtgatct cacggtcatt
     2461 attaaagaag cggatggcag tgaacaacgc tttatttagc cattctcatc ggtgccaatt
     2521 ttccagcgtg aaggccatct caaatatagc tttgccgcag gtgaatatca ggccgggaat
     2581 tatgacagcg cctcgccgcg tttcgggcag cttgatctga tctacggttt accgtggggg
     2641 atgacggcct acggcggcgt attaatctct aataattaca atgcatttac attagggata
     2701 gggaaaaact ttggttatat cggggcgatt tccattgatg tgacgcaggc taaaagcgaa
     2761 ctgaataacg atcgcgatag ccagggacaa tcttatcgtt tcttatattc caagagcttc
     2821 gaaagcggca ccgatttccg ccttgcgggc tatcggtact ctaccagcgg tttctatacc
     2881 ttccaggaag ccaccgatgt gcgcagtgac gctgacagcg actataaccg ttatcacaag
     2941 cgcagcgaaa tacagggtaa cctgacgcag caattagggg cctatggctc tgtttattta
     3001 aatttaacgc agcaggatta ctggaacgac gcaggtaaac agaacacggt atcggcgggt
     3061 tacaacggac gtattggcaa ggtcagttac agtattgcat atagctggaa taaaagccct
     3121 gaatgggatg aaagcgatcg cttgtggtct ttcaatattt ccgttccact aggccgggcc
     3181 tggagtaact atcgcgtcac gaccgaccag gatggtcgta ccaatcaaca ggttggggtc
     3241 agcggaacgc tgcttgagga tcgcaacctg agctacagtg tccaggaagg ctacgccagc
     3301 aacggtgtgg gtaacagcgg taacgctaac gttggctatc agggtgggtc cggtaatgtc
     3361 aacgtaggct atagctacgg gaaagattac cggcagctca actacagcgt tcgcggcggc
     3421 gtgatagttc atagcgaagg cgtgacgctt tcccaaccgc taggcgaaac catgacgctc
     3481 atctccgtac ccggtgcgcg caatgcccgc gtggtgaata acggcggcgt tcaggttgac
     3541 tggatgggta acgcgatcgt gccttatgcc atgccgtatc gtgaaaacga aatctcactg
     3601 cgtagcgatt cgttgggtga cgatgttgac gttgaaaatg cgttccagaa agtggtgcca
     3661 acgcgtggag cgattgtcag agcgcgtttt gatacccgcg ttggttaccg cgtattaatg
     3721 acgctgcttc gttccgcggg cagcccggtg ccctttggag caacggcaac gctaatcacc
     3781 gataaacaaa acgaggtgag cagtatcgtt ggtgaagaag gacagctcta tattagcgga
     3841 atgccagagg aaggacgggt attgattaaa tggggtaatg acgcgtcgca gcaatgcgtg
     3901 gcgccttata aattatccct ggaattaaaa cagggcggaa ttattcctgt ttcggccaat
     3961 tgccagtaag caaggagagc gaaatgaaag cggcaatagc attatcctta ttaggatgcg
     4021 tattcggatt ttctggcaag gcatttgccg gagatgcgtg ggggccatgt acccccgcgg
     4081 atggcacgac ttatcactat aatgtcgatg tggatgttgg tatacctgat gcggcaaaaa
     4141 acgtcgcagg aacggtttta ccggatgtcc ttaactggtc taatggacaa aacgtatcgc
     4201 taatttgtga atgtcctgat tcttataaga atgaaaaaga tactctagtg cagggggtga
     4261 gtatgcttcc cccttctggc cgtacggttg acagtatgaa atattatact ttgacggagg
     4321 aactggaggt tgcgacgaac attcgcatca gtaccagtgt gtatgggttt gttcctttca
     4381 aaaaccagca ggcgctgcaa acaaccggat gcaacaaagt cattactacg ccctatatgg
     4441 gcggcgcagg tctactttcc tttgctatta ctaaaccctt tatcggcgat tccgtcatcc
     4501 ctctgacgtt aattgccgaa ctgtatgcct cgaaaacaaa taaagattac ggaacaatac
     4561 ccatatcttc cgtatctatt caggggcgtg tcaccgtaac ccaggattgc gaaattaaac
     4621 ccggcacggt gctggatgtg ccatttggtg aattcccctc ctcggcgttt aaaaacaggc
     4681 aagggcaaat gcctgaaggt gcgacagagc aagaaattaa cctcagcttc gattgcaaca
     4741 atatctctga tggtatcaag gttgcgttgc gccttgaggg ggcaaccaat gctgacgatc
     4801 cccgagcggt ggatatgggc aaccccgata ttggcgtact ggtgaaagat tccagcggca
     4861 aaattttggt accgaatgat tcaagcagca ccacgttatt aaatttgagc agcctggatt
     4921 ctaaaactca ccgcaatgcc gcgattcgac tgctggcttt accgattagc acgacgggta
     4981 aagcgcctaa aggcgggacg tttgaaggcg taacgaccat atatcttgaa atggagtgat
     5041 gccatgaagt tcaaacgatt gctgcatagc ggcatcgcca gtttgagtct ggttgcctgc
     5101 ggggtgaatg cggcgacgga tcttggcccg gcaggggata ttcatttctc catcactatc
     5161 accactaaag cttgcgagat ggaaaaaagc gatctcgaag tcgatatggg aacaatgacg
     5221 ctgcaaaaac ctgcggcagt cggtacggtg ttgagcaaga aagatttcac cattgaactc
     5281 aaagagtgcg atgggatatc caaagcgacc gttgagatgg acagtcagtc ggacagcgat
     5341 gatgattcca tgtttgccct tgaggctggt ggcgcaacgg gtgttgcgtt gaagatagag
     5401 gacgataaag gaacgcagca agttcccaaa ggctccagcg gaacgccgat tgaatgggcg
     5461 attgatggcg aaaccacgtc gcttcactac caggcgagtt atgtggtcgt caacactcag
     5521 gccactggtg gcacagcgaa tgcccttgtc aatttttcca tcacctatga gtaagttact
     5581 gggccagcat cctggcccaa cctatctctg aatagtcgtt aatccgttaa aatggtctat
     5641 tccggacttg cacttttgta aatttgtgct tcatgcacac tctttcccca cactttttcc
     5701 ctttgctgtg gtctacttat tcgcgcgtgt agattttact tatctgacta cctccgcact
     5761 tttcccctgc tgggcctgaa aagccgctaa gcagggtgtt atcacctgtt tgtccagggt
     5821 ttgtttgcat gagatacatc aaatcgatta cacagcagaa gctgagcttt ttgcttgcaa
     5881 tctatattgg cctttttatg aatggcgcgg ttttttaccg ccgcttcggc agctatgcgc
     5941 acgattttac cgtctggaaa ggcatttctg ctgttgttga actggccgcc accgtactgg
     6001 tgaccttctt tttactacgt cttctttcgc tgtttggccg ccgcagctgg cgtattctgg
     6061 catcgctggt tgtgctcttt tccgcaggtg ccagctatta catgaccttc cttaatgtgg
     6121 tcattggtta tggcatcatc gcttccgtca tgaccaccga tatcgacctg tcaaaagaag
     6181 ttgttggtct gaactttatt ctctggttaa tcgccgttag cgcattgcct cttatcctta
     6241 tctggaataa ccgctgtcgc tacaccttgc tccgacaact gcgaacccca gggcagcgta
     6301 ttcgcagcct ggcggtcgtc gtactggcgg gaattatggt ttgggcaccg attcgtttgc
     6361 tggatatcca gcagaagaaa gtggagaggg cgaccggcgt tgatttgccg agttatggcg
     6421 gtgtcgtagc gaactcttat ctgccatcaa actggctttc tgcgttgggg ctgtacgcct
     6481 gggcgcgggt cgatgaatct tccgataata attcattgct taacccggcg aagaaattca
     6541 cttatcaggc accgcaaaat gttaatgata cttatgtcgt gtttatcatc ggtgaaacca
     6601 cgcgttggga ccatatgggc attttcggct atgagcgtaa taccacgccg aagctggccc
     6661 aggagaaaaa tctggcggcg ttccgtggtt actcctgtga taccgcaacc aaactctctc
     6721 tgcgttgcat gtttgtacgt caggggggcg cggaagataa tccgcagcgc acattaaaag
     6781 aacagaacat tttcgcggta ctgaagcagt taggattcag ttctgacctc tacgctatgc
     6841 agagcgaaat gtggttctac agcaatacga tggcggacaa tattgcttac cgtgaacaga
     6901 ttggtgcgga gccacgtaac cgtggcaagc cggtagatga tatgttgctg gtagacgaaa
     6961 tgcagcaatc gctggggcgc aacccggatg gtaagcatct aatcattctg cataccaaag
     7021 gttcgcactt taactacacc cagcgttacc cgcgcagctt tgcgcagtgg aagccggaat
     7081 gtattggtgt tgatagcgga tgtaccaaag cgcagatgat caactcctat gacaactcgg
     7141 tgacctatgt ggatcacttt atctccagtg tgattgatca ggttcgcgat aagaaagcga
     7201 ttgtgttcta cgcagctgac cacggcgagt caattaatga acgcgagcac ctgcacggca
     7261 cgccgcgtga actggcaccg ccggagcagt tccgcgtacc gatgatggtc tggatgtcag
     7321 ataaatatct ggaaaatccg gtcaatgcgc aggcgtttgc gcagctgaaa aaagcagccg
     7381 acatgaaagt gccacgccgt cacgtagagc tgtacgacac catcatgggt tgtcttggct
     7441 atacttcacc ggatggtgga attaacgaaa acaacaactg gtgtcacatc ccgcagacaa
     7501 aagaggcagc ggctaactaa gaggcctgct gactttctcg ccgatcaaaa ggcattttgc
     7561 tattaaggga ttgacgaggg cgtatctgcg cagtaagatg cgccccgcat tcggtgattg
     7621 gcgcagcctg gtagcgcact tcgttcggga cgaaggggtc ggaggttcga atcctctatc
     7681 accgaccaaa ttcgaaaagc ctgctcaacg agcaggcttt tttgcatctg cagcccatga
     7741 ggaagagaac ctccgggggc agggaggttc gactcgacgt aggcctgata agacgcgtca
     7801 gcgtcgcatc aggcaacggc accgcactta ttgtcggatg cggcgtgaac gccttattcg
     7861 acctacacgc cacacatttc cctgccaaat ttgcgcccca tatctcacta tccattaacg
     7921 gatttgtgac agaaaccatt gtcttttttt atatttgctt aaatcttttt ttgcgttact
     7981 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
     8041 atatccacct tttcctcaga tttttttcac taaatgcata aaacttaatc accaaatgtt
     8101 gctaaatatt aagctgattg ttctggtcat gttggagtag cacaaatttc tctgctgcaa
     8161 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
     8221 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
     8281 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
     8341 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
     8401 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
     8461 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
     8521 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
     8581 gtaccaccta tgacgcctct tccgtaccgc tttataaccg cctagttgaa tttaaaatcg
     8641 gcaccaccga agtgattccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
     8701 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
     8761 cgcgcgaaat gaatgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
     8821 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggcatg ggcttgccgg
     8881 agctgatcag cgaagtgaaa aaggtggacg acaacaccgt tcagtttgtg ctgactcgcc
     8941 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
     9001 atgctgatgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggca
     9061 ccggtccgtt ccagttacag cagtatcaaa aagattcccg tatccgctac aaagcgtttg
     9121 atggctactg gggcaccaaa ccgcagatcg atacgctggt tttctctatt acccctgacg
     9181 cttccgtgcg ttacgctaaa ttgcagaaaa atgaatgcca ggtgatgccg tacccgaacc
     9241 cggcagatat cgcccgcatg aagcaggata aatctatcaa cctgatggaa atgccggggc
     9301 tgaacgtcgg ttatctctcg tataacgtgc agaaaaaacc actcgatgac gtgaaagttc
     9361 gccaggctct gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
     9421 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
     9481 aggactacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa
     9541 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
     9601 gccgcatggc ggagatgatt caggcagact gggcgaaagt cggcgtgcag gccaaaattg
     9661 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgagcac cagacggtaa
     9721 tgatgggctg gactggcgat aacggggatc cggataactt cttcgccact ctgttcagct
     9781 gcgccgcctc tgaacaaggc tccaactact caaaatggtg ctacaaaccg tttgaagatc
     9841 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
     9901 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
     9961 aaccggtacg caaagaagtt aaaggctatg tggttgatcc attaggcaaa catcacttcg
    10021 aaaacgtctc tatcgaataa ttaaaagcca tacccggtgg tgctgtgcct ctgcgtgcag
    10081 cgccatccgg cagcaatact tcattccctg cccgacctgg gcgggaattg atttgtgagc
    10141 aatacagaca cgcagttcca ggctgcgggt cactacagag aatccgggtt atgttgcagt
    10201 ttattctccg acgtttggga ctcgtcatcc ccacgtttat cggtattacc cttctcacat
    10261 ttgcctttgt ccacatgatc ccgggcgatc ctgtgatgat catggcgggc gaacgtggga
    10321 tctccccaga gcgtcacgcg cagttgctgg ctgaactcgg cttagataaa ccgatgtggc
    10381 agcagtatct ccattacatt tggggcgtta tgcatggcga tctaggcatt tcaatgaaaa
    10441 gccgaattcc ggtatgggaa gagttcgtgc cgcgctttca ggccacgctg gaacttggcg
    10501 tctgcgcgat gatttttgcg accgcggtcg gcattccggt tggtgtgctg gccgcggtta
    10561 aacgcggttc cattttcgat cacacagcgg ttggcctggc gctgacaggt tattcaatgc
    10621 ctatcttctg gtggggcatg atgctgatca tgctggtttc ggtgcactgg aacctgacac
    10681 ccgtctccgg tcgcgtgagc gatatggtgt tcctcgatga ttccaatccg ttaaccggtt
    10741 ttatgctgat cgacaccgcc atctggggtg aagacggtaa ctttatcgat gccgtcgccc
    10801 atatgatctt gcccgccatt gtgctgggta ctattccgct ggcggtcatt gtgcgtatga
    10861 cacgctcctc gatgctggaa gtgctgggtg aagattacat ccgcaccgcg cgcgccaaag
    10921 ggttgacccg catgcgggtg attatcgtcc atgcgctgcg taacgcgatg ctgccggtag
    10981 tgaccgttat cggcctccag gtgggaacat tgctggcggg ggcgattctg accgaaacca
    11041 tcttctcgtg gcccggtctg gggcgctggt tgattgacgc actgcaacgg cgcgattatc
    11101 cggtggtgca gggcggcgta ttgctggtgg cgacgatgat tatcctcgtc aacctgctgg
    11161 tcgatctgct gtatggcgtg gtgaacccgc gtattcgtca taagaagtaa ggggtcatca
    11221 tgtcacaggt tactgaaaat aaagtgatta gcgcgccggt accgatgacc cctttacagg
    11281 agttctggca ctattttaaa cgcaacaaag gcgcggtcgt cgggctggtt tacgtcgtca
    11341 tcgtgctgtt tatcgcgatc tttgccaact ggattgctcc ctataacccg gcggaacagt
    11401 tccgcgatgc actgctcgcc ccgccagcct ggcaggaagg cggcagcatg gcgcacttgc
    11461 tgggtaccga tgatgtaggc cgtgatgtgc tgtcgcggct gatgtacggc gcgcgcttgt
    11521 cgctgctggt tggctgtctg gtggtggtgt tatcgctgat tatgggcgtt attctcggcc
    11581 tgatcgccgg ttacttcggc ggcctggtcg ataacatcat tatgcgcgtg gtcgatatca
    11641 tgctggcgct gccaagtctg ctgttggcac tggtgctggt ggcgattttc ggcccgtcga
    11701 ttggtaacgc cgcgctggcg ctgacctttg ttgccttgcc gcactatgtg cgcttaaccc
    11761 gcgccgccgt gctggtggag gtgaatcgcg attacgtcac cgcttctcgc gtggcaggag
    11821 ccggggcgat gcgccagatg tttattaaca tcttcccgaa ctgccttgca ccgctgattg
    11881 ttcaggcgtc gctcggtttc tctaacgcca ttctcgatat ggctgctctt ggtttcctcg
    11941 gcatgggggc gcagccgcca acgccggagt ggggcaccat gctctccgac gtgttgcagt
    12001 tcgcgcaaag cgcctggtgg gtcgtgacct tcccgggtct ggcgatcctg ctgacggtgc
    12061 tggcatttaa cctgatgggt gacggtctgc gtgacgcgct cgatcccaaa ctgaagcagt
    12121 aagaggttcg agatggcgtt attaaatgta gataaattat cggtgcattt cggcgacgaa
    12181 agcgcaccgt tccgcgccgt agaccgcatt agctacagcg taaaacaggg cgaagtagtc
    12241 gggattgtgg gtgagtctgg ctccggtaag tcggtcagtt cgctggcgat tatggggctg
    12301 attgattatc cgggccgcgt gatggcggaa aagctggagt ttaacggtca ggatttgcag
    12361 cgtatctcgg aaaaagagcg ccgcaacctg gtgggtgccg aagtggcgat gatcttccag
    12421 gacccgatga ccagccttaa cccgtgctac accgtgggtt tccagattat ggaagcgatt
    12481 aaggtgcatc agggcggtaa taagagcact cgtcgtcagc gggcgattga cttgttgaat
    12541 caggtcggta ttcccgatcc ggcttcgcgt ctggatgttt acccgcatca gctttccggc
    12601 ggcatgagcc agcgcgtgat gattgcgatg gccattgcct gtcggccaaa actgctgatt
    12661 gccgatgaac caaccactgc gctggacgtg accattcagg cgcaaatcat tgaactactg
    12721 ctggagctac agcagaaaga gaacatggcg ctggtgttaa ttacccatga cctggcgctg
    12781 gtggcggaag cggcacataa aatcatcgtg atgtatgccg gtcaggtggt ggaaacgggc
    12841 gatgcgcacg ccatcttcca tgcgccgcgt cacccgtata ctcaggcatt gctgcgtgcg
    12901 ctgccggaat ttgctcagga caaagaacgt ctggcatcgt tgcctggcgt tgttcccggc
    12961 aagtacgacc gcccgaacgg ctgcttgctt aacccgcgct gcccctatgc cactgacaga
    13021 tgtcgcgctg aagaaccggc gctgaatatg ctcgctgacg ggcgtcagtc caaatgccat
    13081 tacccacttg atgatgccgg gaggccgaca ctatgagtac gcaagaggcc acctcgcaac
    13141 aaccgctgtt gcaggctatc gacctgaaaa aacattatcc ggtgaagaaa ggtatgttcg
    13201 ctccggaacg tctggtgaag gcgctggacg gcgtttcgtt taaccttgaa cgtggcaaaa
    13261 cgctggcggt agtaggtgaa tctggctgcg gtaaatcgac cctcggtcgg ttgctgacga
    13321 tgattgaaac gcccaccggc ggcgagctgt attaccaggg gcaggatctg cttaagcacg
    13381 atccgcaggc gcagaagctg cgtcggcaga aaatccagat tgtcttccag aacccttacg
    13441 gttcgctgaa tccgcgtaaa aaaatcgggc aaattcttga agagccgctg ctgatcaaca
    13501 ccagcttaag caaagaacag cgtcgggaaa aagccctgtc gatgatggcg aaagtcggcc
    13561 tgaaaaccga gcactatgac cgctatccgc atatgttctc cggcggtcag cgtcagcgta
    13621 tcgccattgc ccgaggtctg atgctcgacc cggacgtggt gattgccgat gaaccggtct
    13681 ccgcgctgga cgtctcggtg cgtgcacagg tgctcaacct gatgatggat ttgcagcagg
    13741 aactggggct ttcttatgtc ttcatctccc acgacctgtc ggtggtggag cacattgctg
    13801 atgaagtgat ggtgatgtac ctgggccgct gcgtggagaa gggaacgaaa gaccaaatct
    13861 tcaataaccc gcgccatccg tacactcagg cgctactttc cgcgacgccg cgcctgaatc
    13921 cggacgatcg ccgcgagcgc atcaaactca ccggtgaact gccaagcccg ctgaatccac
    13981 cgccgggctg cgcgtttaac gcccgctgtc gtcggcgctt cggcccctgc acccagttgc
    14041 agccgcagct aaaagactac ggcggtcaac tggtagcttg ttttgctgtt gatcaggatg
    14101 aaaatccgca gcgttaactc acaaaaccgg ggccatgcgc tccggtttac tcaatcagct
    14161 tcaacagtgg cgacaagcat agcaaaatgc cgtaaagcag aatcagccag gttttaaatc
    14221 cgcgaagttt ttccagttgt gcgactttat agatcaggaa aaacggaatg aggcacgaca
    14281 caatgccata taacgggctt cccaactgaa agaacaccag caccgataca cgaaacgaaa
    14341 cccaaatcgt caacgtaatg acgataaaag cgcagatcgc cagagtcagc acgcgtgagt
    14401 taattttctt ggtatcaata attcggctta acagattgag aataatgcct ttaatggcct
    14461 cgtggaaacc gagataaatg ccaaagaatg cggtcagtac ggcaaagata ttcagcaccg
    14521 tagaggtgat atgaatgata tgcccaggga tcacctgcgc ggccagtgcc agtgccgaga
    14581 tattttgttc aaaggcagaa acggcttctt cgtggctaat tgagaaggta aacgaaaagg
    14641 caaaaaacag tatcaccgcg atgagcgtga tataactaat tcggtgggta cgcagcgcga
    14701 gccgggttgc cagtacttta tccgcttccc gtttacgata ggcaatattc attgggttaa
    14761 gtacctgaat aaatactgca gaaaagaagc aaaatggaat ggtaagcagg acatcgcgaa
    14821 aaaagacgga ggcttgcggg aaggcggtta tattggcgaa attccagtgc gggatcatcg
    14881 taaaaccgaa cacgacaata atccctactt tgaccaccac cattggccca gaaatcttaa
    14941 acagtaatcg ttcaccacca gacgcaatcg ctaccagtac ggcgaaaata gcgactttat
    15001 aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa ggttttcagg tacgaggcgc
    15061 tgtcgaaaac cacggagaga gagtagataa aaatcccgtg gataatcatc aaaaagtaga
    15121 taaccccgag gaaaattccc cagttcttcc ccaggtaatg actgataata tcggtgtagt
    15181 cattacagga atcgctttca gaaagggttt ttaaataaat gtcctgcact acccaggtgg
    15241 caggataagc aatgatcgct gcggtaataa atacccaaat gcccttcaag ccaatttgta
    15301 ctggcatcag cacggttccg gcaccaatcg ccatgcctat gcataataaa acccagccga
    15361 agtcgtagcg tgtaaacggc aatttctgat cgtgtttcgg tagtgtgttg tgctgcataa
    15421 agtcctgtcc agaaagaagt cggcctcgca aaaatgaata aacccagttt ctcttgcgca
    15481 acaggtggag aagtcgaata cgcagtatgt aaaaaatgag gcaacagatt cactggggag
    15541 gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac taccagtctg gagtcagccc
    15601 ggatatgaac tgcaactatt gcaaattaag aaattaagct ggaaggcatc acgcaatgag
    15661 gaagattgcc gagtgctgac agtaaaaagc aatcgtaaaa ttagcggcaa gccggaaggg
    15721 ttccggtgag gcgcaatgtt gcgggggctt aatccctggt ggcattggtt gctggagaga
    15781 gaaaaccccc gcacgttgca ggtatgcacc tgacaacacc acgggggcta atcttgaacc
    15841 tagaccactc aagaatagcc gcgaaacgtt gtcattacaa cacaggcggc tatatgacgc
    15901 tcgcagagct gggcatggcc ttctggcatg atttagcggc tccggtcatt gctggcattc
    15961 ttgccagtat gatcgtgaac tggctgaaca agcggaagta acgtgtcatg cgggcgtcag
    16021 gctgccgtaa tggcaatttg cgcccggacc agaccgcagg ggggaaactc tgcggccttt
    16081 ttcgtgctta ctgcgggtaa ggcacccagt cgccgccgtt caggcgaacg tacggtttat
    16141 cctggtattg aataactact gcatttgagt tctctgagac cggtgctgtt tgtggcaacc
    16201 cactggtgag ttttttccag tcaacattgt cttcggtgaa aatcttgcca tcaagaacgc
    16261 gaaccaccag atcggagata gccaggaagc tgctcggttg gtcgatgaca atcggtgccc
    16321 cctgatgtgg tgccttcatg ccgaagaatt tcaccccaac ggggacgtcg gtgatagacg
    16381 ggctagggat atcacgtagg ccagatacct gcattctgtc gcccttcagc gcgccgccgt
    16441 gttccggcac cacgaccacc atcactttac gacccgattt ctccagttca gtaaagaagg
    16501 cgtccagttc atcaaagaat ttctgcgccc gcgctttgta atccgctgtt ttgctgacgc
    16561 ccggataatg gttgccgtca tgcagtggaa gcgtgttgta gaacgtggca ctacggctgt
    16621 ttttatcttt ttcggtaacg tccagccagc ggttaagcac ggcggtatcg tcataaaccg
    16681 gcgaaccatc aaagcccagc aaaataaccg gcagatttgt ttgatccatc aattcagtct
    16741 gcatgccgcc attttcgcga acttctttca aaaaaccgcc gaactggccg ttatgtccca
    16801 tcatcaggtg ctgggtaaag cccagtttcg acaggttatc aaacagatag cagtcgttat
    16861 ttgccggttg atacagatta gtgtgcgaag tctgcccgca gctggcgcgc agtaaacgga
    16921 tcgccgccgg gccactgtag gaggtggcgg agttaaagtt cttgaactca atatcgaaat
    16981 gcgaccacag tggatgcgac atcaacccag cggcttctat atccgaccag gaaagcgaac
    17041 agatgttaat caccagtagt tcaaatggct gagcatcagc gggcagcgaa gacgggaagg
    17101 tcgatttacg tttcgcctcc gcgttataga aattattcag ccaggcgtta aggttcgccg
    17161 ttgttggcgg tgcagtttgt gcgggcatat cacccactac cggtgcgcca cccgtcgccg
    17221 caacggttgc cgctgcgtta ccacccgtcg ttgttacagt ggtcgtcggt tgtccggctg
    17281 gccacaagga gaaacttggt cccgccaggg taagtacgtt cagccatagc agtatggcaa
    17341 ccacaaaaac ggtaatgcga atccattgtg acaggaataa ccaggccact aataaaacaa
    17401 aaatggcccc aatcatctgc cagttaataa agcgtgtgac aaggtcgatt aaataatcgg
    17461 tactgaaccc cgccacctgc gaaccctggc tcattatgct ttccgggcca ggcaaccagg
    17521 tgtcatgcca gaacaaagca aagccgatcg gcagggcaat ccagtggcgc aagcgatgca
    17581 ggctgtagcg cggaatgggc atcagcagaa acgcggcaaa caccaaattg aggagcggat
    17641 ggaagttaag atatcccgcc cacaacaggc cgaacttaac cagaaaatag aagttccagc
    17701 cggaaaggcc gcgccagtat tgccagaggg aagaaggcat ggcggtattt tgcgtaaatt
    17761 gagtcatttt ttggttgccc tggctttttc cgtgcggcgt aacgtcccag ccggtttaac
    17821 ataacgaggt ttagcaaaga acaaacgtaa ggtgtcgcga atgcgtcgca aagagtgccg
    17881 cgccagatag cccagcggga aaaatatcag tgcgcaaacg acaataattt caatgatatc
    17941 gctgatggtc atcatgatga gcgctccaca gcatcatcta acagtcgcat gggttctggt
    18001 attcgtcgcc agtggcgacc atcgtgctcg gcattgatga ccggtttaga actttgcgtt
    18061 aaaggcagcg gcatgcccca ttgttctggg gcaagcaggc gcatctgcac cagctcggca
    18121 ctgatttgat catcttcaaa ccagaccata cggtttgaga aaatgtcgcc agtaggcaat
    18181 gggaaaatat gattcaacgc ggtatccaga tcgttaatcc gacagaatga gagaaacagc
    18241 accagccgat taccgccaat ggtcatgata tcgccggtac ggttagggcg acacagcgtc
    18301 agggcttgtt caacgcggat acccggtacc ggacgtaggg caaccagaac gcctttaccg
    18361 tgggcaggta atagagggtt attcatcatg ttgttgacgg cattacagaa cacatcccac
    18421 ttctggaaac cacgcagttt gagcggctgg gtcattgaca gcaaggtagt gatatcttcc
    18481 ggcacatagc gactaaactt ctgcccttgc acgctttcga tcatcgtcag acaacgggag
    18541 agtggcgcat tccacggaat aaccatattt gcaccgcagg ccaataacaa acgttcatcg
    18601 gtggcgcgca ggctagcggt attttcccgc acgaggattt tcatcgcact accgcgctgg
    18661 cgacgcaggg tatgaatgct gcgggccagt ggctcgattt gcgcattttg ctgtaaagaa
    18721 aagaccaccg tcgccgcctg agcggtacgg gcttcattga acaggacttc gttattgttg
    18781 aacagttgcc agtgttccga tagcggcggc gcaccttcca gtacagcaac attactcaga
    18841 atgcgttttt cgtcgctgcg tggttggatc tccgcctctt cgctttgaac taatgtccag
    18901 ataccatttt gttgctgaac gctaagctgc tgacgggcgc tgaccccttt ttcgttgcac
    18961 cagaaggcaa tatccagcaa atgttggtcg ccctgaaaac gcaaactggc aagaccaaaa
    19021 agtgaacggt attcctcaag caacaatgaa aattgtttat cgttattatt tccgggatta
    19081 attaccaaaa gcgaacaatg gtttaacctg ctccatttat tcattttatc caaccatgag
    19141 cgcagccgct cggcaggaat gttttgccat gcgttatttg cgcaaacaag aataaaaagg
    19201 taattatggg gatcaataga acactgcaaa tcacgggcca aatagtatag acctttttca
    19261 tgatttagca ttgaaaataa ttttattttt tccggccctt gagaatcatc taattgaaag
    19321 attttcgccg gatcgctgtc catgctaatg accgcgacgt gtgcggtttc agcctgggat
    19381 gcaattgttt gattcgccag actgatagca tcttcatggc gatcgacgtt aaaccaccag
    19441 acgccgcctg ctggcatatg tcgcagctca tcccataatg atgagatacc gatagagaat
    19501 acagggtcca caatgtccct catgtcacca ttttgtctga ttatcagttt actagcgaaa
    19561 gctaagaaat aaacctacca ttgaaattaa aacttatcag atttagcatg tgaatcaatt
    19621 tagcaggtca caagacgtgt gattttagtc ttttcgtttt taactttttc tgttgcgtaa
    19681 cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa acaataaacc ggcgaaatgc
    19741 tcacggttag aattgtctca tgaacggtac ggttatttca tagggatcaa gcaaaatgaa
    19801 taacaatgaa ccagatactc tgcctgatcc cgcgataggc tatatcttcc agaatgatat
    19861 tttggcgtta aagcaggcat tttcactgcc tggtattgat tatgccgata tttcccaacg
    19921 cgaacagttg gccgcggcat taaaacgctg gccgttgctg gcagagtttg cgcaacaaaa
    19981 gtaggggatt ggtgaatggc cgtactggga ttgcaggggg tgcggggagg cgtggggaca
    20041 acaaccatca ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgtcctggtg
    20101 gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta atgttgattt tacccaccgt
    20161 cagggctggg ccagagctat gctggatggc taggactggc gcgacgctgg gttgcgctac
    20221 acctcgcagc tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca
    20281 cagcactggc aaacccggct gagcgatatt tgctccggct tacagcaact aaaagccagc
    20341 gggcgttacc agtggatttt aatcgactta ccgcgtgatg cctcgcagat aacccaccag
    20401 ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg tggatgccaa ctgccatatc
    20461 cgactgcatc agcaagcgct gccggatggc gcacatattt tgattaataa cttccgtatt
    20521 ggcagtcagg ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattactg
    20581 ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc tggcggctaa gcaaccagta
    20641 ggtgaatatc gcagtgatgc gctggcggct gaagagatat tgacgctggc gaactggtgc
    20701 ctgttgaact attccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc
    20761 ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg gcgttatcgc gattatcgtc
    20821 gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga
    20881 tttttattcc actggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt
    20941 atccgcatat caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa
    21001 catgctggtt actgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc aggcgggcat
    21061 tttcaggtct gcagaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc
    21121 gcgttagcca taaaacacag catcttgatg agaaaaaaga gctcggtcat ttgagtgccg
    21181 gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc gctgattctg gcgttaatct
    21241 gcgttactca gccgtttaac ccgctggcgc agtttatctt cctgatgctg ctgtgggggg
    21301 gagcgctgat cgtacggcgg atgccggggc gcttctcagc gctaatgttg attgtgctgt
    21361 cgctgactgt ttcttgccgt tatatctggt ggcgatatac ctctacgctg aactgggacg
    21421 atccggtcag cctggtgtgc gggcttattc tgctcttcgc tgaaacgtac gcgtggattg
    21481 tgctggtgct cggctacttc caggtagtat ggccgctgaa tcgtcagccg gtgccattgc
    21541 cgaaagatat gtcgctgtgg ccgtcggtgg atatctttgt cccgacttac aacgaagatc
    21601 tcaacgtggt gaaaaatacc atttacgcct cgctgggtat cgactggccg aaagacaagc
    21661 tgaacatctg gatcctcgat gatggcggca gggaagagtt tcgccagttt gcgcaaaacg
    21721 tgggggtgaa gtatatcgcc cgtaccactc atgaacatgc gaaagcgggc aacatcaaca
    21781 atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt cgactgcgac cacgtaccaa
    21841 cgcgatcgtt cctgcaaatg accgtgggct ggttcctgaa agagaaacag ctggcgatga
    21901 tgcagacccc acaccatttc ttctcgccgg acccgtttga acgcaacctg gggcgttttc
    21961 gtaaaacacc gaacgaaggc acgctgttct atggtctggt gcaggatggc aacgatatgt
    22021 gggacgccac tttcttctgc ggttcctgtg cggtgattcg ccgtaagccg ctggatgaaa
    22081 ttggcggcat tgctgtcgaa actgtgactg aagatgcgca tacttctctg cgtttgcacc
    22141 gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc ggcggggctg gcgaccgaaa
    22201 gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg cgggatggtg caaatcttcc
    22261 gtctcgataa cccgctcacc ggtaaagggc tgaagtttgc tcagcggctg tgctacgtca
    22321 acgccatgtt ccacttcttg tcgggcattc cacggctgat cttcctgact gcgccgctgg
    22381 cgttcctgct gcttcatgcc tacatcatct atgcgccagc gttgatgatc gccctgttcg
    22441 tgctgccgca tatgatccat gccagcctga ccaactccaa gatccagggc aaatatcgcc
    22501 actctttctg gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc
    22561 tggtggcgct gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg
    22621 tggaagaaga gtacgtcgac tgggtgatct cgcggcccta catcttcctt gttctgctca
    22681 acctggtggg cgttgcggta ggcatctggc gctacttcta tggcccgcca accgagatgc
    22741 tcaccgtggt cgtcagtatg gtgtgggtat tctacaacct gattgttctt ggcggcgcag
    22801 ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca ccgcgtggag atgacgatgc
    22861 ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag gatttctccg
    22921 acggtggttt ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga
    22981 atctgttgct taaacgcggt cagcaggaat acgtcttccc gacccaggtg gcgcgcgtga
    23041 tgggtaatga agttgggctg aaattaatgc cgctcaccac ccagcaacat atcgattttg
    23101 tgcagtgtac gtttgcccgt gcggatacat gggcgctctg gcaggacagc tacccggaag
    23161 ataagccgct ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc
    23221 tggcggagtt tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt
    23281 cctgggttgt atcgtttatt ccgcgccgcc cggagcggag cgaaacggca caaccatcgg
    23341 atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa gaaaactatt ctggatttgt
    23401 gcagtggcta tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg
    23461 caaccactga tcaatgctga gccagctgta gccgcccaga cggaacaaaa tccgcaggtg
    23521 gggcaagtga tgccgggcgt gcagggcgct gatgcgccag tcgtggcgca gaacggtcct
    23581 tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc cgccgggcag catggtgcta
    23641 cgtggcatta acccgaacgg cagcattgag tttggtatgc gcagcgatga agtggtgacg
    23701 aaggcgatgc tcaacctcga atacacgcca tcgccatcgt tactgcctgt ccagtcgcag
    23761 ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc cagtgaccaa agaacagttg
    23821 ggtaaaaaaa cgctggcgca aatgcccatt aatccactgt ttattaccga cttcaaccgt
    23881 gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc cagcaccacg
    23941 ctttggctgg atgttgggcg gagcagtgga ctggatctga cctatcagac cctgaatgtg
    24001 aagaatgacc tgtcacactt cccggtgcca ttctttgacc cgcgtgataa ccgcaccaac
    24061 accttgccga tggtctttgc gggtgcgccg gatgttgggc tgcaacaagc atctgccatt
    24121 gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc agaacttccc ggtgctctat
    24181 aaccaactgc cggatcgtaa tgccattgtc tttgcaacca acgacaaacg gccggacttc
    24241 ctgcgcgatc atccggcggt aaaagccccg gtgattgaga tgattaacca tccgcagaat
    24301 ccttacgtca aactgctggt ggtgtttggt cgtgacgaca aagacctgtt gcaggcagcg
    24361 aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa gcgtggtagt gaatgaagtg
    24421 aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact gggtgcgtac cgatcgtccg
    24481 gtcacctttg gtgaactgaa aacctatgaa gaacagttac aatccagcgg tcttgagcca
    24541 gcagcgatta atgtttcgct aaacctgccg ccagatctct acctgatgcg cagtaccggc
    24601 attgatatgg atattaatta ccgctacacc atgccgccgg tgaaagacag ttcgcggatg
    24661 gatatcagcc tgaataacca gttcctgcaa tccttcaacc tgagcagcaa acaggaggcg
    24721 aaccgcctgc tgctgcggat tccggtatta caaggtttgc tggatggcaa aacggatgtc
    24781 tctattccgg cgctgaaact gggcgcgacc aaccagctgc gcttcgactt tgagtatatg
    24841 aacccgatgc ctggcggttc ggtggataac tgtattacct tccagccggt gcagaatcat
    24901 gtggtgattg gtgacgactc caccatcgac ttctcgaagt attaccactt catcccgatg
    24961 ccggatctac gcgcctttgc taacgcgggc ttcccgttca gccggatggc ggatctgtcg
    25021 caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac agatggaaac gttgctgaat
    25081 actgttggtt ttatcggcgc acagacgggc ttcccggcga ttaatctgac ggtgaccgat
    25141 gatggcagca ccattcaggg caaagatgcc gacatcatga tcatcggtgg tatcccggac
    25201 aaactgaaag acgataagca gatcgaccta ttggtgcagg cgaccgaaag ctgggtgaaa
    25261 acaccgatgc gccagacccc gttccccggc attgtgccgg acgagagcga tcgcgcggca
    25321 gaaacccagt caacgctgac ctcttccggt gcgatggcgg cggtgattgg cttccagtcg
    25381 ccgtataacg accagcgcag cgtgattgcg ctgctggcag atagcccacg cggttatgaa
    25441 atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg
    25501 gtgatccgcg agtccggtat caacagccta cgtgttggcg acgtttatta cgtaggtcat
    25561 ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc atccgattct gctggcggtg
    25621 ctggcggtgc tggcggtgct ggcggtgctg gcggtgctgg cggctatcag tgtgatattg
    25681 ctggcatggg tactgtggcg tttgctgcgt attatcagcc gtcgtcgtct taacccggat
    25741 aacgagtaat tgaagatgaa tgtgttgcgt agtggactcg tgacgatgct gctgttggct
    25801 gcctttagtg ttcaggcagc ctgtacctgg cctgcctggg agcagtttaa aaaggattac
    25861 atcagtcagg aagggcgcgt catcgacccc agcgacgcgc gcaaaatcac cacctctgaa
    25921 gggcaaagtt acggcatgtt ctttgccctg gcggctaacg accgtgcagc tttcgataat
    25981 attctcgact ggacgcagaa caatctcgct cagggttctt taaaagaacg tttgcccgcc
    26041 tggctgtggg gcaagaaaga gaacagtaag tgggaagtgc tggacagcaa ttcggcctcc
    26101 gatggtgatg tctggatggc ttggtcgttg ctggaggcgg ggcgtttgtg gaaagagcag
    26161 cgttataccg acatcggcag cgcgttgcta aaacgtattg cgcgggagga agtggtgacg
    26221 gtgcctgggc tgggttccat gttgttaccg ggcaaagtgg gttttgctga ggataacagc
    26281 tggcgtttta accccagcta cctgccgccg acgctggcgc agtatttcac ccgctttggc
    26341 gcgccgtgga ctacgctgcg cgaaaccaat caacgtttat tgctggaaac cgccccgaaa
    26401 ggcttttcgc cagactgggt gcgctatgag aaagacaaag gctggcagct aaaagccgaa
    26461 aaaacattga tcagcagcta cgacgctatc cgcgtttaca tgtgggtagg catgatgcct
    26521 gacagcgatc cgcagaaagc gcggatgctc aaccggttta aaccgatggc gacattcact
    26581 gagaaaaacg gttatccgcc ggaaaaagtg gatgtggcta cggggaaagc gcagggtaaa
    26641 ggaccggtcg gtttttctgc cgccatgctg ccctttttac aaaaccgtga tgcgcaggcc
    26701 gttcagcgcc agcgcgtggc cgataacttt cccggcagcg atgcctatta caactatgtg
    26761 ctgaccctgt ttggacaagg ctgggatcaa caccgtttcc gcttctcgac aaaaggtgag
    26821 ttattacctg actggggcca ggaatgcgca aattcacact aaacatattc acgctttccc
    26881 tcggtctggc cgtcatgccg atggtcgagg cagcaccaac cgctcagcaa cagttgctgg
    26941 agcaagttcg gttaggcgaa gcgacccatc gtgaagacct ggtgcagcag tcgttgtatc
    27001 ggctggaact tattgatccg aataacccgg acgtcgttgc cgcccgtttc cgttctttgt
    27061 tacgtcaggg cgatattgat ggcgcgcaaa aacagctcga tcggctgtcg cagttagcgc
    27121 cgagttcaaa tgcgtataaa tcgtcgcgga ctacgatgct actttccacg ccggatggtc
    27181 gtcaggcact gcaacaggca agattgcagg cgactaccgg tcatgcggaa gaagctgtgg
    27241 cgagttacaa caaactgttc aacggtgcgc cgccggaagg tgacattgct gtcgagtact
    27301 ggagtacggt ggcgaaaatt ccggctcgcc gtggcgaagc gattaatcag ttaaaacgca
    27361 tcaatgcgga tgcaccgggc aatacgggcc tgcaaaacaa tctggcgcta ttgctgttta
    27421 gtagcgatcg ccgtgacgaa ggttttgccg tcctggaaca gatggcgaaa tcgaacgccg
    27481 ggcgcgaagg ggcctctaaa atctggtacg ggcagattaa agacatgccc gtcagtgatg
    27541 ccagtgtgtc ggcgctgaaa aaatatcttt ctatctttag tgatggcgat agcgtggcgg
    27601 ctgcgcaatc gcaactggca gaacagcaaa aacagctggc cgatcctgct ttccgcgctc
    27661 gtgcgcaagg tttagcggcg gtggactctg gtatggcggg taaagccatt cccgaactac
    27721 aacaggcagt gcgggcaaac ccgaaagaca gtgaagctct gggggcgctg ggccaggcat
    27781 attcacagaa aggcgatcgc gccaatgcag tggcgaatct ggaaaaagcc ctcgcactgg
    27841 atccgcacag cagcaacaac gacaaatgga acagtctgct gaaagtaaac cgctactggc
    27901 tggcgatcca gcagggcgat gctgcgctga aagccaataa tcctgaccgg gcagaacgcc
    27961 tgttccagca ggcgcgtaat gtcgataaca ccgacagtta tgcagtgctg gggctgggcg
    28021 atgtggcgat ggcgcgaaaa gattatcccg ccgccgaacg ttattatcag cagaccttgc
    28081 gtatggacag cggcaacact aacgccgtgc gcgggctggc aaatatttac cgccagcaat
    28141 cgccagaaaa agctgaagcg tttatcgcct cactctctgc cagtcagcgg cgtagcattg
    28201 atgatatcga acgcagcctg caaaacgacc gtctggcaca gcaggcagag gcactggaaa
    28261 accagggcaa atgggcgcag gcggcagcac ttcagcggca acgactggcg ctggaccccg
    28321 gcagcgtatg gattacttac cgactttcgc aggatctctg gcaggccgga caacgcagcc
    28381 aggccgatac gttaatgcgc aatctggcgc agcagaagcc gaacgacccg gagcaggttt
    28441 acgcttacgg gctgtacctc tctggtcatg acctggacag agcggcgctg gcgcatatca
    28501 acagcctgcc gcgtgcgcag tggagcagca atattcagga gctggttaat cgactgcaaa
    28561 gcgatcaggt gctggaaacc gctaatcgcc tgcgagaaag cggcaaagaa gctgaagcgg
    28621 aagcgatgct gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg
    28681 cgctgcagcg acgtgattac accgccgccc gcgctgcata tcagaatgtc ctgacgcggg
    28741 agccaactaa cgccgatgcc attcttggtc tgacggaagt ggatattgct gccggtgaca
    28801 cagcggcggc acgtagccag ctggcgaaac tgcccgctac tgataacgcc tcgctgaaca
    28861 cacagcggcg cgtggcgctg gcgcaggcgc agcttggaga taccgcagcg gcgcagcaga
    28921 cgtttaataa gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg
    28981 tgctgcgcga cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag gcgctggaaa
    29041 cctacaaaga cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca
    29101 cctttacccg actgacccgt aacgacgaga aagatgactg gctgaaacgc ggcgtgcgca
    29161 gcgatgcggc ggacctctat cgccagcagg atcttaacgt caccctcgag catgattatt
    29221 ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg
    29281 tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg
    29341 tcggcagttt ctccactaat gccgatggca aatgggatga caactggggc acctgtacat
    29401 tacgggactg tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct
    29461 ggcgaaatga cgtctggagc tgggatatcg gcaccacgcc gatgggcttc aacgtggtgg
    29521 atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg
    29581 cccaccgtcg tcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga
    29641 gcaataccgg gaaaaaatgg ggcggcgtgc gtgccgacgg cgtggggcta agcctgagct
    29701 acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta
    29761 aaaatgtcga agataactgg cgtgtgcgct ggatgacggg ctattactat aaggtcatca
    29821 accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag
    29881 acctgagtgg ctactcactc ggtcagggcg gttactacag cccgcaggaa tacctgtcgt
    29941 ttgccatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt
    30001 ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga
    30061 tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg
    30121 gcttcggcta tacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgtcg
    30181 gcacggcaat tgatatccag caggcgaaag attacgcccc cagccatttc ctgctctacg
    30241 tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac
    30301 cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat
    30361 actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa
    30421 caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca
    30481 ccgttttgct gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa
    30541 gcattgctcg ctctgtccgc gaacctttat cttcagccat tttgaaaggt gatattcccg
    30601 aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag
    30661 tgctgcctaa ccagttccag gcgctgcgta aaagttttat tccagagcgt ccggtgccgg
    30721 taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtc tactcgctcg
    30781 aacgtccggc aaatccgcag ccaattgcct atctggtgct acaggcggat tccttccgta
    30841 tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta cttttgtcgt
    30901 taatattgac ggtggcgatt agctggtgca ttaaccgcct gattttgcat ccgttacgca
    30961 atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat
    31021 taccgcgtct gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc
    31081 agcaattgct gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt
    31141 cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac
    31201 aaaccaccgc gctgatgatc atcacctgtg aaactctgcg tgatactgcg ggcgtgctga
    31261 aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt
    31321 cgccacgtat gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg
    31381 tacatgaacc gtggcacgca atcactttgg gtcagcaagt gctcactatc atgagcgagc
    31441 gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattagcgtg gcgatgttct
    31501 acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc
    31561 gccataaagg caagaatcag attcagttct ttgatccgca gcagatggaa gccgcccaga
    31621 agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcag tttgctattt
    31681 ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc
    31741 gtatccagca accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt
    31801 gctgtgggct gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg
    31861 cagcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctct gtgctgcaac
    31921 tgatgcaccc gaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc
    31981 cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg
    32041 tggcaatcct ccgtccgctg cgtaatgccg gagttcgggt ggcgctggat gatttcggca
    32101 tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgttctga
    32161 aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta
    32221 tcatgctggc gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac
    32281 aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcgtc
    32341 cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg
    32401 attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt
    32461 gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc
    32521 aggtacccca taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc
    32581 tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg
    32641 ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct
    32701 cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat
    32761 ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt
    32821 cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg
    32881 aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc
    32941 gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc
    33001 atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct
    33061 ccaccgtctg ggcagcaaag gccaactgat ttttaacgtc atcgaaagtt tctcgcaggt
    33121 catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgt tcggggcaat
    33181 ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat
    33241 ctgtttctac attacctgta tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc
    33301 gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact
    33361 ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg
    33421 ctgccgtaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg
    33481 cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat
    33541 ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc
    33601 aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt
    33661 gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc
    33721 gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga
    33781 actggaccac aaaaaactgg acgatgcgct gaataatcgt gcgccggatg gcaaaacgca
    33841 cgaattatcc tcttaatctc atcacttatg cccgcactcc cttccccgag tgcgggtgtt
    33901 tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac
    33961 ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta
    34021 gactgaccga caacgggcag tttttttatt accaggatag ttgatcaggg gttcacatgc
    34081 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    34141 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    34201 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    34261 tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    34321 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    34381 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    34441 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaggcg ctctcttatc
    34501 tggcaaatgc cactggcaaa ctgactatca cgccagaaac catcaaccac gcgctgcaaa
    34561 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    34621 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    34681 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctgctgg
    34741 tggtgggaaa cgtggatgcg cgctcggtcg tcgaccaaat caacaaaacg tttggcgaac
    34801 tgaaaggcaa acgtgaaaca ccggctccgg tgccgacgct ttctccgctg cgtgcggaag
    34861 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    34921 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    34981 gtgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    35041 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    35101 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    35161 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    35221 tgcagaaact gtttgccgcc tatgcacggg ctgataccga tattctgatg ggccagcgga
    35281 tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    35341 aggatttcct taacagcctg actgtggaga tgttaaatca ggatctgcgt cagcagttgt
    35401 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    35461 cgttgcaggc ggcctgggat caaatcatgg ccccatctac cgccgctgcg accacctctg
    35521 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    35581 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaactc
    35641 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    35701 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgct gcgttttccg
    35761 cactgccgcc cgtcaaacgt accgccagat aaccggcact gaaagagtcg ccagctgcgg
    35821 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    35881 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    35941 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    36001 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    36061 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    36121 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    36181 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    36241 ccgcggattg atcgctctcc agccagaatt tggcggcggc ttcgttacgc cagtagtaga
    36301 acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    36361 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atctgctggc
    36421 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    36481 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    36541 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    36601 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    36661 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    36721 ttgtggagtg gggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    36781 atctttgtcg agtccgggct gcatcacttt taaacacagg acatctttga tgataaggca
    36841 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    36901 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcgggcggtt
    36961 aatggccgtg gagctattaa cggcggtcac gcatcccttg aacccttcgc aacgcctgcc
    37021 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    37081 gattgatttg ctggcgcaaa aagccgactt ctttatagag cacggcctgc tggcatcggt
    37141 caatattgat ggtcctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat
    37201 tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc cgaaagattc
    37261 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gtaccgggat
    37321 ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagaact
    37381 gtttgtgatg ctgcgtcagt cgccggaagg acgcacactc ttttctcagc ttttacatct
    37441 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacgccgg aagagtggcg
    37501 tgatgtacag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    37561 gatagaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgcctggct
    37621 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    37681 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    37741 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    37801 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaagca
    37861 agaaactggc tggcgcagct gggtgccgtg gccccatgta cacgcggaag acatcattct
    37921 tggcaatcca ccggatattc ccgaagtcac gatggtgcat ttgccacgcg tagaagcaac
    37981 gctggccccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa
    38041 gcccgacgcg cgcctgattc gcctctctga aaagaacaat aactggacgt ttaatctcgc
    38101 caacgatgat aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    38161 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    38221 gatttttgtt gatcccttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    38281 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    38341 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    38401 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    38461 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    38521 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    38581 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    38641 tcgcggcttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    38701 cggcaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    38761 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    38821 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    38881 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    38941 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    39001 cttgcgcctg caaccgctga aatttggcat tgcgggtggc agcattgcgg cgaatattca
    39061 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    39121 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    39181 tgacgcggaa ctgcgcggta gcggtaactc ggtggcggca cttttaggca acagtaacgg
    39241 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    39301 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    39361 ctgcgcggcg gcgaatctga atattgccaa cggcgtggca cgcccgcaga tttttgcttt
    39421 cgatactgag aacgcgttga ttaacgttac cggcacggca agttttgctt cggaacagct
    39481 ggatttgact attgatccgg agagtaaagg tattcggatt atcacactgc gttcgccgct
    39541 gtatgtgcgt gggacgttta aaaatcctca ggctggggtg aaagccgggc cgttgattgc
    39601 ccgtggtgct gttgctgcgg cactggcaac gctggttacg ccagcggcag cgttgctggc
    39661 actgatctca ccttccgaag gggaggctaa tcagtgccgg acgattttgt cgcagatgaa
    39721 gaagtgaaga taattgccgg atgtctgaca tccggcaatt aaacagattt agattgttgg
    39781 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    39841 tgcgaattca atatattgta tggaaattca ggcctgataa gcgtagcgca tcaggctttt
    39901 cactcttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    39961 aagccagcca tcgccgccag atataacccc accgcactta gcccgtagtt agtctgcaac
    40021 caggctgcga tatatggcgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaac
    40081 gatgctccgg tgtaacgcac ttctgtcgga aacagctctg gtaacagcgc acccattggc
    40141 ccgaaggtca gacccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    40201 ttgccagaac cgagcagtgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    40261 atgattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccagc gactggcacc
    40321 atcacgccaa aaccaataac tgccatcatc aacatccaca acacttcgtt acgcggcagg
    40381 ccaagcccaa ctggcgcggc ggcggtacta aaggtcatag agtagaccgt catgatgtaa
    40441 aacagcgtat aggttgccag cataatgaac gtacccagta cggttacgcg aacatgtttg
    40501 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    40561 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag caccgccgag
    40621 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    40681 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgcgggaag
    40741 gagccgtaca atgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    40801 ccccattcac cacctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    40861 gcgaaaatac caatcgtggc atagcctggc agcagaccaa tcaccacggt cgaaatcccc
    40921 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    40981 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    41041 agcgttgctg ccgtaggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    41101 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    41161 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    41221 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    41281 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    41341 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    41401 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    41461 agttggcatg aaattgtttg ctcctgaata gatggctgaa gaggctctgg gaaagagttt
    41521 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat tcggcctacg
    41581 gggagccagg ccgaataaat tcgtatgccg gataaggcgt tttgaactgc atccggccta
    41641 ttttaaggct gcgttttccc cggcattcgc gggtcgtctt tatattcggc ggtggcaatc
    41701 cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat cagccccagc
    41761 acggaaccaa atgctgcgcc agagggggat ttcatcaacg atggcagggt gtaggtcatc
    41821 acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat cagggctttt
    41881 ttgcgcggac ggtggcgcgg caaccgccag aagatccaga agaaaagcag atagttggcg
    41941 aagatggaaa tcgccaggcc aatcaaccgc cacgtcggtt tcagccactc aatgctgttc
    42001 aggtgcaggg cgctaataat catttgctgc gccgaaccgg caaccgaggt gatcgaaagc
    42061 gtcacaatca gcgcaatcaa caaaccaatc agcgaaataa aatcacgcag atattttacc
    42121 cagaacttct cctgatcttg cggcgagcgt tcccagacat cgcgcgactg ggcacgaatc
    42181 gcttcacgca ggttacccat ccagttgatg ccggaataaa gcgccaccgc caggccgaca
    42241 agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa cgtagcggct
    42301 agcgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag caacatcgga
    42361 tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg gatcatcgac
    42421 aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc gttaaagcgt
    42481 tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt ggactttttc ggcagtggtg
    42541 gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc caactgctta
    42601 atcggctcgt gctccagatc ctgggtggga cgtttgatct cgttttcctg cgtcatccgc
    42661 tcttttgtcc ttctgtcgtg gtcattctta aaaagtatag tcggtcagtc caggtactct
    42721 ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag ctcccgacgc
    42781 tgcggataaa ccagcgaaag ggagagcggc tcggcacggt agcctggcaa cacttcaata
    42841 agccgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat aatccccaga
    42901 ccggcaagac cggcggcgag atagttttcg ctactgttta ccgtcaacat gccgccagac
    42961 ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg tacacccagg
    43021 tgcggtgtat aacgcactat tgcgtgtgaa gtgagatcgt cggggttttg cggataacca
    43081 aagcgcgtca gatagtgcgg actggcacag ttgaccatgg tcagtttgcc gaggggacgg
    43141 gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa accatcatga
    43201 agaatatcta ccggacggtc atggctactc agttccagct caattcccgg atgctgatag
    43261 agaaattccg acaggtgcgg cagtaacagg ctttttgcga ttcctggcgg gatgtcgatg
    43321 cgtaatttac cgctgatact ggtggcatcc tgctgaaaca gaccgtccag ttcgttgaga
    43381 ttactcaaca catcttttgc tcgttgataa taggtcatgc cttctggcgt gagtttgacc
    43441 cgtcgcgtgg tgcgctggag aagctgggtg ccaagttgat gttccagtgc ctgtatctgg
    43501 cgcgaaacac ttccttttgg caaagcaaag aaatccgctg cgcgggaaaa actttccagc
    43561 tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt gaccgattgt
    43621 tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttattttttc taacatctaa
    43681 tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg aaaagggcgg
    43741 aatagctttc tgagaaaggt gaatcccctt aaacagagcg tatttttcag cgattccctt
    43801 taaagtgaat agaggacata ttggctattt gtgcttattt ttgtctcatt gccatttgtt
    43861 attttggctt gtggtcgcgg aatttcattt ttaattgcat gcgtactaac caccaatata
    43921 aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag gcgtttagct
    43981 gtaactattt atatcggtaa ctattttcta cgcgccatct ctgtagaatg gccaccgcca
    44041 aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa gtctttttaa
    44101 cgtattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag tcaatattta
    44161 ttcatggaat gaaaatcagt ttacagcagc gtattccagg agtgagtatt cagggggcca
    44221 gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta gttatgctcg
    44281 atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg gtgcaatttc
    44341 ctgaggttaa ggtgttaatt acggcgacgg actgcaataa acggtggtta caggaagtta
    44401 tccattttaa tgtgctggcc attgtgcctc gtgattcaac cgtcgagaca tttgcgttgg
    44461 cggtaaatag tgcggcgatg gggataatgt ttctgcctgg agactggcgg acgacgccgg
    44521 aaaaggacat taaagatctc aaatcgttaa gtgcccgcca gcgggagatt ttaaccatgt
    44581 tagcggcggg cgaatcaaat aaggagatcg gcagagcgct gaatatcagt accggaacgg
    44641 taaaagcgca tctggaatcc ctgtatcgcc gtctggaagt gaaaaatcgc acccaggcgg
    44701 caatgatgtt aaatatctcc tcctgaagag gataatttta cagccagcag gaagttagcg
    44761 gctggctgta aaaatattat ggttcgccgt acaaaccaat taaacggcgt accacaccgt
    44821 tagtccagcc aaacccatcc tgcaacggat actcgccacc gccgccttcg cggggaacac
    44881 catcggcaat atggtatttt tcgatcagtt tgtgctgttc cagataaaac tgattcaccg
    44941 tcttcagcca gctgcgcgcg atttcatcgc ccagaaggtc atcgccatac attttaaatc
    45001 cctgaattgc catccattgt aacggtgccc agccgttggg tttatcccac tgttcaccgg
    45061 tttcgtactc gcttgccaga atcccgccag gtgtcagtaa ccgactgcgc acggcgtttg
    45121 ccagacgatc ggcctgttca tggttcgcca taccgacata gagcggcaca atggcggcag
    45181 cggaaaacag cgccagttgt tcgcgtcgcc agtcgtaatc gcggtagatg ccgttttcat
    45241 catcccagag gtaacggttt accgcatcgc gacgggcact ggccttctgg cggaacagtg
    45301 cttctgtctc tttctcacct ttcagcgccg agatgttggc gatggcgctc tccagtttaa
    45361 acaggaaggc attcagatcg atggggatga actgggtggt acgaatgctc gccagacgac
    45421 cagtatcacg gagccaacgg gaagagtaat cccaaccgga ggccgccccc gcgcgtaaat
    45481 cgcggtacac ctcgttgggc gggcgaccag aatgtttcgc ggtttcaacg tcctcaagcc
    45541 aggattcgtc acgcggcgtg tcgcgatcgt cccagtaacg gttgagcagc gatccgtccg
    45601 gcatccgcac aacatggcga taggcctgat tagggatcaa cgattctgca ccgtccatcc
    45661 agaaggcata ttccattttc aggtggtcga gatagcggcg cgcaccgcgt acaccatctt
    45721 cttcaaacaa ctccaccatc agcgcaaaaa ccggtggttg cgagcggctc aaataatagg
    45781 tgcggttgcc gttggggatg tgaccgtagt tttcgatcat ccaggcgaag ttatcggcca
    45841 tgcatttcag caaatcttcc cgaccacttt ccgccagccc cagcatggtg aaataggaat
    45901 cccaatagta ggtttcgcta aaacggccgc ccgggacaat atatgactgc ggcagcgcca
    45961 gcagagaaga ccacggaatg tgatcctgtg gttcgcgggt tagcaccggc cacagctggt
    46021 cgatatgctc tttcagggaa ttttgcgggt ccgatacata ctcgctggag tagacctccg
    46081 gcagccagaa gtgattttca acaaacttgc gcaagtcaaa atcacgatga cggcgcactt
    46141 tacggtagcg gattaaaata tccagcgggt ccattttggg tgcgcagtcg ggaaaggttt
    46201 tactgtcggg gaaaattttc gccgactgaa catgctcgaa cagttcgaga tagcgatcgg
    46261 caggcgtcag cgcatcagag gcgggcagcc cttcaatcat ctcgggttcc ggttctgcct
    46321 cgatcatctc atccagtttt aattcatacg ggtccagttc atagcagaga tcgacctcga
    46381 tcatcagttc gtctggatta gggttttgaa ttttctgatt gagcataagg atccaacctc
    46441 cgaaattcgt cgctaattat cgggcttttc tttaagggta gtcattcgct ccgcctggtg
    46501 ggcgacaaag cgtgcggtgg atcacgttta ttaacctgtc aaaaaatata acgcactaat
    46561 atcaatggaa tttataaaaa tacagatttc gtgttgtttt tcattttcag aacattcaat
    46621 cctgctcttg ccgcaatcat gatatgccat tcctgatctg ggtaataggc acaggctatc
    46681 ttattgatag tttatattca tgtaattgat tgattcctat caaaaagcgt atctccctgt
    46741 tttttatgat ggctttgtct cgcagagacg tttgcagcat tctggaaaat tttctgggtc
    46801 acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat taccgcgatc
    46861 ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg gtaccacgat
    46921 aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa taataaggtt
    46981 ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc agaattaccc
    47041 gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa gcttggatat
    47101 aaatctttta acctcgaggc cgtgcgtgct gctctgctgg ctgataaacc cgtttcgcaa
    47161 agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccgcc aacgcgttat
    47221 accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat actggcctgg
    47281 attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga gcatcgcaat
    47341 gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt ggcgttgggc
    47401 tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg cgctcattgc
    47461 catgcgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt tggtggcgca
    47521 gttgggccga ttaacgcgcc gacggtattt aactcagtat ttaacgttga gcagttctgg
    47581 gatggtcgtg cggcaacatt gcaggatcag gctggtggac cgccgttgaa cccgattgaa
    47641 atggcgtcaa aatcctggga cgaaattatt gctaagctgg aaaaagatcc gcagcttaaa
    47701 gcgcagttcc tcgacgtcta tccgcaaggt ttcagtggcg aaaatattac tgatgccatt
    47761 gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg gttgcgtggg
    47821 gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa agataataaa
    47881 tgtgcaactt gtcatggtgg aattattctc ggcggacgtt ccttcgaacc gttggggctg
    47941 aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat gaatgtgact
    48001 aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt tgctttaacg
    48061 gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa actgatgttg
    48121 cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt agctttcctg
    48181 cacagtctga acggggttta cacgccgtat atgcaggata aacaataatt aatttgatcg
    48241 cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt ttaatattat
    48301 catattaaat gtttatatta taaaagatca tttttctgct taggattttg ttatttaaat
    48361 taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt gccttcaaat
    48421 aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc agaactactc
    48481 gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg atttccgctg
    48541 cacgaaatgc gcgatgatgt cgcattccag attatcaatg atgaattata tcttgatggc
    48601 aacgctcgtc agaacctggc cactttctgc cagacctggg acgacgaaaa cgtccataaa
    48661 ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc gcaatccgca
    48721 gccatcgacc tgcgttgcgt aaacatggtt gccgatctgt ggcatgcgcc tgcgccgaaa
    48781 aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcttgtat gcttggcggg
    48841 atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggcaaacc aacggataaa
    48901 ccaaacctgg tgtgcggtcc ggtgcaaatc tgctggcata aattcgctcg ctactgggat
    48961 gtggagctgc gcgagatccc tatgcgcccc ggtcagttgt ttatggaccc gaaacgcatg
    49021 attgaagcct gcgacgaaaa caccatcggc gtggtgccga ctttcggcgt gacctacacc
    49081 ggtaactatg agttcccgca accgctgcac gatgcactgg ataaattcca ggccgacacc
    49141 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
    49201 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
    49261 cataaattcg gtctggctcc gctgggctgc ggctgggtga tctggcgtga tgaagaagcg
    49321 ctgccgcagg aactggtgtt caacgttgac tacctcggtg gtcagattgg tactttcgcc
    49381 atcaacttct cccgcccggc gggtcaggtg attgcgcagt attatgaatt cctgcgcctc
    49441 ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
    49501 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgtcc ggacgaaggc
    49561 atcccggcgg tttgcttcaa actgaaagag ggtgaagatc cgggatacac cctgtacgac
    49621 ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct cggcggtgaa
    49681 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
    49741 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
    49801 cagggtattg cccagcagaa cagctttaaa cacacctgat aacataacgt tgtaaaaacc
    49861 gaatgcccag cctttaaaaa aaacagctgg gcattcggtt gcttattaat cgcaataata
    49921 tattggctgt ctattcatcg tgttgataag atatatgtta tatgaatgtt tattaaatgt
    49981 agcgattacc tttaatgtca cgacaaggga ttatttgctt actattaatt tccctgcgtt
    50041 cttaaacacg tttttgtatt ataaatccag tcatcctgcc cgtagaacat cacacattat
    50101 catcctgttc tcccgcttcg tttaaattta tttatcaatc aatttgactt aagagggcgg
    50161 cgtgctacat taataaacag taatatgttt atgtaatatt aagtcaacta tgcaatcatt
    50221 acatgggaat tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg
    50281 tgaatatcgc tattttaatg gcggtgacct ggtttttgcg gatgcaagcc aaattcgagt
    50341 agataagtgt gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctccc
    50401 gatgctcaag gaggaggcat taaatcttca tgcacataaa aaagtttctt cattactcgt
    50461 tcatcactgt agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa
    50521 gaatcttcgc tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt
    50581 ttttcttgag gatgatcact ttataccgct gcttctgaac gttttacaac cgaacatgcg
    50641 aacacgagtt tgtacggtta tcaataataa tatcgctcat gagtggacac tagcccgaat
    50701 cgccagcgag ctgttgatga gtccaagtct gttaaagaaa aaattacgcg aagaagagac
    50761 atcatattca cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat
    50821 acatggtttt tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt
    50881 catttacgtc tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc
    50941 gcagggattg ccgaaccgtg actcggcggc aagtattgtt gcgcaaggga atttttacgg
    51001 cactgactgt tctgcggaag gaataagatt atagagtttt actcagacat aaaaaaaacc
    51061 cggcataggg gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac
    51121 actccctgtt ggcacgggaa actttgtgct ctcagtaagt taaatataac ttttactgga
    51181 aataagatca gccatttttt tataaacata agctatacgc tgtgcgaaaa tatattcgtg
    51241 ttgc
//
LOCUS       DABSYT020000022        50835 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.022, whole genome shotgun sequence.
ACCESSION   DABSYT020000022 DABSYT020000000
VERSION     DABSYT020000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 50835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.022"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            18..920
                     /locus_tag="ID725_RS08450"
                     /old_locus_tag="pgaptmp_001690"
     CDS             18..920
                     /locus_tag="ID725_RS08450"
                     /old_locus_tag="pgaptmp_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7850313.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNECF"
     gene            1067..2287
                     /locus_tag="ID725_RS08455"
                     /old_locus_tag="pgaptmp_001691"
     CDS             1067..2287
                     /locus_tag="ID725_RS08455"
                     /old_locus_tag="pgaptmp_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGV7850314.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2260..2889
                     /locus_tag="ID725_RS08460"
                     /old_locus_tag="pgaptmp_001692"
     CDS             2260..2889
                     /locus_tag="ID725_RS08460"
                     /old_locus_tag="pgaptmp_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGV7850315.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2890..4050)
                     /gene="ybdL"
                     /locus_tag="ID725_RS08465"
                     /old_locus_tag="pgaptmp_001693"
     CDS             complement(2890..4050)
                     /gene="ybdL"
                     /locus_tag="ID725_RS08465"
                     /old_locus_tag="pgaptmp_001693"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGV7850316.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4159..5247
                     /gene="hcxA"
                     /locus_tag="ID725_RS08470"
                     /old_locus_tag="pgaptmp_001694"
     CDS             4159..5247
                     /gene="hcxA"
                     /locus_tag="ID725_RS08470"
                     /old_locus_tag="pgaptmp_001694"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGV7850317.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5257..5454)
                     /locus_tag="ID725_RS08475"
                     /old_locus_tag="pgaptmp_001695"
     CDS             complement(5257..5454)
                     /locus_tag="ID725_RS08475"
                     /old_locus_tag="pgaptmp_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGV7850318.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5636..7741)
                     /gene="cstA"
                     /locus_tag="ID725_RS08480"
                     /old_locus_tag="pgaptmp_001696"
     CDS             complement(5636..7741)
                     /gene="cstA"
                     /locus_tag="ID725_RS08480"
                     /old_locus_tag="pgaptmp_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGV7850319.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(7922..8335)
                     /gene="entH"
                     /locus_tag="ID725_RS08485"
                     /old_locus_tag="pgaptmp_001697"
     CDS             complement(7922..8335)
                     /gene="entH"
                     /locus_tag="ID725_RS08485"
                     /old_locus_tag="pgaptmp_001697"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGV7850320.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8338..9084)
                     /gene="entA"
                     /locus_tag="ID725_RS08490"
                     /old_locus_tag="pgaptmp_001698"
     CDS             complement(8338..9084)
                     /gene="entA"
                     /locus_tag="ID725_RS08490"
                     /old_locus_tag="pgaptmp_001698"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGV7850321.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9084..9941)
                     /gene="entB"
                     /locus_tag="ID725_RS08495"
                     /old_locus_tag="pgaptmp_001699"
     CDS             complement(9084..9941)
                     /gene="entB"
                     /locus_tag="ID725_RS08495"
                     /old_locus_tag="pgaptmp_001699"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGV7850322.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9955..11565)
                     /gene="entE"
                     /locus_tag="ID725_RS08500"
                     /old_locus_tag="pgaptmp_001700"
     CDS             complement(9955..11565)
                     /gene="entE"
                     /locus_tag="ID725_RS08500"
                     /old_locus_tag="pgaptmp_001700"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGV7850323.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11575..12750)
                     /gene="entC"
                     /locus_tag="ID725_RS08505"
                     /old_locus_tag="pgaptmp_001701"
     CDS             complement(11575..12750)
                     /gene="entC"
                     /locus_tag="ID725_RS08505"
                     /old_locus_tag="pgaptmp_001701"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGV7850324.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12939..13895
                     /gene="fepB"
                     /locus_tag="ID725_RS08510"
                     /old_locus_tag="pgaptmp_001702"
     CDS             12939..13895
                     /gene="fepB"
                     /locus_tag="ID725_RS08510"
                     /old_locus_tag="pgaptmp_001702"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGV7850325.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13899..15149)
                     /gene="entS"
                     /locus_tag="ID725_RS08515"
                     /old_locus_tag="pgaptmp_001703"
     CDS             complement(13899..15149)
                     /gene="entS"
                     /locus_tag="ID725_RS08515"
                     /old_locus_tag="pgaptmp_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGV7850326.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            15260..16264
                     /gene="fepD"
                     /locus_tag="ID725_RS08520"
                     /old_locus_tag="pgaptmp_001704"
     CDS             15260..16264
                     /gene="fepD"
                     /locus_tag="ID725_RS08520"
                     /old_locus_tag="pgaptmp_001704"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGV7850327.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16261..17253
                     /gene="fepG"
                     /locus_tag="ID725_RS08525"
                     /old_locus_tag="pgaptmp_001705"
     CDS             16261..17253
                     /gene="fepG"
                     /locus_tag="ID725_RS08525"
                     /old_locus_tag="pgaptmp_001705"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGV7850328.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17250..18065
                     /gene="fepC"
                     /locus_tag="ID725_RS08530"
                     /old_locus_tag="pgaptmp_001706"
     CDS             17250..18065
                     /gene="fepC"
                     /locus_tag="ID725_RS08530"
                     /old_locus_tag="pgaptmp_001706"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGV7850329.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18062..19195)
                     /gene="wzz(fepE)"
                     /locus_tag="ID725_RS08535"
                     /old_locus_tag="pgaptmp_001707"
     CDS             complement(18062..19195)
                     /gene="wzz(fepE)"
                     /locus_tag="ID725_RS08535"
                     /old_locus_tag="pgaptmp_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGV7850330.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19411..23292)
                     /gene="entF"
                     /locus_tag="ID725_RS08540"
                     /old_locus_tag="pgaptmp_001708"
     CDS             complement(19411..23292)
                     /gene="entF"
                     /locus_tag="ID725_RS08540"
                     /old_locus_tag="pgaptmp_001708"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGV7850331.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(23289..23507)
                     /locus_tag="ID725_RS08545"
                     /old_locus_tag="pgaptmp_001709"
     CDS             complement(23289..23507)
                     /locus_tag="ID725_RS08545"
                     /old_locus_tag="pgaptmp_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HGV7850332.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(23510..24712)
                     /gene="fes"
                     /locus_tag="ID725_RS08550"
                     /old_locus_tag="pgaptmp_001710"
     CDS             complement(23510..24712)
                     /gene="fes"
                     /locus_tag="ID725_RS08550"
                     /old_locus_tag="pgaptmp_001710"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGV7850333.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24955..27195
                     /gene="fepA"
                     /locus_tag="ID725_RS08555"
                     /old_locus_tag="pgaptmp_001711"
     CDS             24955..27195
                     /gene="fepA"
                     /locus_tag="ID725_RS08555"
                     /old_locus_tag="pgaptmp_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGV7850334.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27370..27990
                     /gene="entD"
                     /locus_tag="ID725_RS08560"
                     /old_locus_tag="pgaptmp_001712"
     CDS             27370..27990
                     /gene="entD"
                     /locus_tag="ID725_RS08560"
                     /old_locus_tag="pgaptmp_001712"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGV7850335.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28112..28264)
                     /gene="hokE"
                     /locus_tag="ID725_RS08565"
                     /old_locus_tag="pgaptmp_001713"
     CDS             complement(28112..28264)
                     /gene="hokE"
                     /locus_tag="ID725_RS08565"
                     /old_locus_tag="pgaptmp_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGV7850336.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(28615..28767)
                     /gene="hokE"
                     /locus_tag="ID725_RS08570"
                     /old_locus_tag="pgaptmp_001714"
     CDS             complement(28615..28767)
                     /gene="hokE"
                     /locus_tag="ID725_RS08570"
                     /old_locus_tag="pgaptmp_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGV7850337.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            28736..29003
                     /locus_tag="ID725_RS08575"
                     /old_locus_tag="pgaptmp_001715"
                     /pseudo
     CDS             28736..29003
                     /locus_tag="ID725_RS08575"
                     /old_locus_tag="pgaptmp_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29206..30324
                     /locus_tag="ID725_RS08580"
                     /old_locus_tag="pgaptmp_001716"
     CDS             29206..30324
                     /locus_tag="ID725_RS08580"
                     /old_locus_tag="pgaptmp_001716"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGV7850338.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            30390..30638
                     /locus_tag="ID725_RS08585"
                     /old_locus_tag="pgaptmp_001717"
     CDS             30390..30638
                     /locus_tag="ID725_RS08585"
                     /old_locus_tag="pgaptmp_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGV7850339.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            30703..31071
                     /locus_tag="ID725_RS08590"
                     /old_locus_tag="pgaptmp_001718"
     CDS             30703..31071
                     /locus_tag="ID725_RS08590"
                     /old_locus_tag="pgaptmp_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGV7850340.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            31165..31818
                     /gene="nfsB"
                     /locus_tag="ID725_RS08595"
                     /old_locus_tag="pgaptmp_001719"
     CDS             31165..31818
                     /gene="nfsB"
                     /locus_tag="ID725_RS08595"
                     /old_locus_tag="pgaptmp_001719"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGV7850341.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31926..33173
                     /gene="ybdG"
                     /locus_tag="ID725_RS08600"
                     /old_locus_tag="pgaptmp_001720"
     CDS             31926..33173
                     /gene="ybdG"
                     /locus_tag="ID725_RS08600"
                     /old_locus_tag="pgaptmp_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGV7850342.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(33241..34617)
                     /gene="pheP"
                     /locus_tag="ID725_RS08605"
                     /old_locus_tag="pgaptmp_001721"
     CDS             complement(33241..34617)
                     /gene="pheP"
                     /locus_tag="ID725_RS08605"
                     /old_locus_tag="pgaptmp_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGV7850343.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(34719..37856)
                     /gene="cusA"
                     /locus_tag="ID725_RS08610"
                     /old_locus_tag="pgaptmp_001722"
     CDS             complement(34719..37856)
                     /gene="cusA"
                     /locus_tag="ID725_RS08610"
                     /old_locus_tag="pgaptmp_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGV7850344.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(37868..39091)
                     /gene="cusB"
                     /locus_tag="ID725_RS08615"
                     /old_locus_tag="pgaptmp_001723"
     CDS             complement(37868..39091)
                     /gene="cusB"
                     /locus_tag="ID725_RS08615"
                     /old_locus_tag="pgaptmp_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGV7850345.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(39107..39439)
                     /gene="cusF"
                     /locus_tag="ID725_RS08620"
                     /old_locus_tag="pgaptmp_001724"
     CDS             complement(39107..39439)
                     /gene="cusF"
                     /locus_tag="ID725_RS08620"
                     /old_locus_tag="pgaptmp_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGV7850346.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(39463..40845)
                     /gene="cusC"
                     /locus_tag="ID725_RS08625"
                     /old_locus_tag="pgaptmp_001725"
     CDS             complement(39463..40845)
                     /gene="cusC"
                     /locus_tag="ID725_RS08625"
                     /old_locus_tag="pgaptmp_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGV7850347.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            41002..41685
                     /gene="cusR"
                     /locus_tag="ID725_RS08630"
                     /old_locus_tag="pgaptmp_001726"
     CDS             41002..41685
                     /gene="cusR"
                     /locus_tag="ID725_RS08630"
                     /old_locus_tag="pgaptmp_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGV7850348.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            41675..43123
                     /gene="cusS"
                     /locus_tag="ID725_RS08635"
                     /old_locus_tag="pgaptmp_001727"
     CDS             41675..43123
                     /gene="cusS"
                     /locus_tag="ID725_RS08635"
                     /old_locus_tag="pgaptmp_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGV7850349.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
     gene            43717..45618
                     /locus_tag="ID725_RS08640"
                     /old_locus_tag="pgaptmp_001728"
     CDS             43717..45618
                     /locus_tag="ID725_RS08640"
                     /old_locus_tag="pgaptmp_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGV7850350.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            45646..46107
                     /locus_tag="ID725_RS08645"
                     /old_locus_tag="pgaptmp_001729"
     CDS             45646..46107
                     /locus_tag="ID725_RS08645"
                     /old_locus_tag="pgaptmp_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGV7850351.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITRDPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            46127..>50835
                     /locus_tag="ID725_RS08650"
                     /old_locus_tag="pgaptmp_001730"
     CDS             46127..>50835
                     /locus_tag="ID725_RS08650"
                     /old_locus_tag="pgaptmp_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGV7850352.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTSAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPAPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDWIKSEAAFNKYINMINKRLELTGSKFRVEIQPAIKNGERVPATTNGPFKVNGKWT
                     SGTHYTGGSKRLDAGII"
BASE COUNT        12251 a        13936 c        13208 g        11440 t
ORIGIN      
        1 tataaccgga gtagtgtatg accaatctct acgacttgaa aaagttcgac ttaaatcttc
       61 ttgtcatatt tgagtgtatt taccagcatt taagcattag caaagctgct gaatcactat
      121 atatcacccc atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc
      181 ctttattcat tcgctcagga aaaggtatcg cgccgacgac aacagggtta aatctgcatc
      241 accatcttga gaaaaatctc agaggccttg aacaaacaat taatatagtc aataagtccg
      301 aactaaagaa aaactttatt attcacggcc ctcagctcat ttcttgctct aataatagta
      361 tgctgatccg ctgcctacgg caagattcct ctgtcgagat tgagtgccat gatatcctca
      421 tgtcagcaga gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc
      481 aaatgccagt gatcagccgt tccgtcattt gcatgccatt gcatacgatt cgcaatactc
      541 taatttgtag taataggcat ccacgaatta ctgacaatag cacttatgaa caaattatgg
      601 cagaagagtt cacccaatta atatctaaat cagcaggggt tgatgatatt caaatggaaa
      661 tagacgagag atttatgaat cgcaaaattt catttcgtgg ttcctctttg ctgacaatca
      721 ttaatagcat tgcagttaca gatttactgg gaatagtccc ctatgaactt tataactccc
      781 accgtgactt tcttaattta aaagagataa aactagaaca tccgttacct tcaataaagc
      841 tttacatttc ctataataaa tcttctttaa acaatctggt gttctcacga tttattgacc
      901 gtctaaatga gtgtttttga tatataacct cagacagcta ctataacttt cacctattta
      961 ttcacagcgc gaatgaaaaa taaaacactc tgcttttcag tgtttacttt ataattaaca
     1021 gtcttatctc actgatcacc ctgtaacgtt cagagagcgt cttcagatgt ctatttataa
     1081 aattcctctt ccgctcaata ttctggaagc tgcacgagag cgtattacct ggacactcaa
     1141 caccctaccc cgcgtttgtg tctcattttc tggcggcaaa gactctggtt taatgctgca
     1201 tctgacagcc gaactcgccc gacaaatggg caaaaaaatc tgcgttttgt ttatcgactg
     1261 ggaggcgcaa ttctcttgca ccattaacta tgttcagtcc ctgcgcgagt tgtacaccga
     1321 tgtcatcgaa gagttttact gggttgcgct cccgcttacg acgcaaaatt ccctttcaca
     1381 ataccaaccc gaatggcagt gctgggaacc tgatgtcgaa tgggtacgtc agcccccgca
     1441 agatgcgata accgatcctg actttttctg cttttaccag ccaggcatga ccttcgaaca
     1501 atttgtacgt gagtttgccg aatggttttc acaaaaacgt ccggcggcga tgatgatcgg
     1561 catccgtgcg gatgagtcct acaaccgttt tgtcgccatc gccagtttaa ataaacaacg
     1621 ttttgccgac gataaaccct ggactacggc cgcaccaggc ggtcatagct ggtacattta
     1681 ccccatttac gactggaaag tggctgatat ctggacctgg tatgcaaatc atcaaagtct
     1741 ctgcaaccca ctgtataacc taatgtatca ggcaggcgtt cctctgcgtc atatgcgaat
     1801 ttgcgaacct tttggcccgg agcaacgaca aggattatgg ctctatcacg ttatcgaacc
     1861 ggatcgctgg gctgctatgt gcgcacgagt cagcggggta aaaagtggcg gcatttacgc
     1921 cggacatgat aatcatttct atgggcatcg gaaaatcctc aagccagaac atttagactg
     1981 gcaagaatat gcattattgc tgctcaatag catgccggaa aaaacagctg agcattaccg
     2041 caataaaatc gccatttatt tgcactggta tcagaaaaaa ggcatcgagg ttccacaaac
     2101 ccagcaaggg gacattggcg cgaaagatat cccctcctgg cggcggatat gcaaagtttt
     2161 actcaataac gattactggt gtcgggcatt atcatttagt cctacgaaag cgaagaacta
     2221 tcagcgttat aacgaacgga taaaaggaaa acgtcaggaa tgggggatac tatgcaacaa
     2281 cgattaacgc aggatttaac gcaatttctc gccagcttgc cggaagatga tcgcatcaac
     2341 gcgattaatg aaatccgcat ggcgatccat caggttagcc cctttcgtga agagccagtg
     2401 gattgcgttc tgtgggttaa aaacagccaa cttatgccta atgattacaa cccaaacaac
     2461 gtggcaccac ccgaaaaaaa actgctgaaa aaatccattg aaatagatgg ttttacccaa
     2521 cccattgtgg tcacgcatac ggataaaaat gccctggaaa tcgtggatgg ttttcaccgc
     2581 catgaaattg gtaaaggctc atcatcatta aaattacgct taaaaggtta tttgccggtg
     2641 acctgtctgg aaggtacgcg taatcaacgc atcgccgcga ctattcgtca taatcgcgct
     2701 cgtggacgcc atcaaatcac cgccatgtcg gaaatcgtcc gtgaactcag tcagttggga
     2761 tgggacgata ataaaattgg caaagagctg ggaatggaca gcgacgaagt gctgcgcctg
     2821 aagcaaatta acggtctgca agagctgttt gccgaccgtc aatattcccg cgcctggact
     2881 gtcaaataac tacagctggc gcagtcgttc agctgctgcc agcaacgtcg attccttctt
     2941 ggcaaaacag agacgaatca gtttatgtgg gaagggatcg gcgcaaaaca ccgatagcgg
     3001 aatcgccgcc acgccgtgct cccgcgtcag ccactggcaa aactcaacat catccagggt
     3061 agaaaccgcg ctgtaatcca ccagcaaaaa gtatgtgcct tcgcacggta aaatctccag
     3121 ccgactttca tttaaggcat ttaccagaat atcgcgcttc tggcgataaa agtccggtaa
     3181 cgcaagataa tgctcaggtt ctgcacgtag catatccgca attgccagct gtgccggggt
     3241 attcaccgaa aaggtcagat actgatgtac cttgcgaatt tcggcgctga tgggcgctgg
     3301 cgcaacacaa taacccactt tccagccggt catatgatag gtcttgccaa acgatgacac
     3361 cgccaccgcc cgctcacgca gctgcggatg cgccagcaca ctggcatggc cctgttgtga
     3421 aaagttgatg tgctcgtaga cttcatcgct aatgacaaaa atctcgtgcc cggcgatcgc
     3481 ctgccacaaa gcggcgaaat cagcctgctg ccagacagtt gcactgggat tatgcggagt
     3541 gttgaggatc accagtctgg tgcgctcgct taacaatgcg gcaaattcct gccagtcaac
     3601 gcgaaaatgc ggtggttgca gtgccatacg cttcactatt cccccagaaa gcgcgatggc
     3661 gggggcgtaa ctgtcatagc tgggatcaaa acaaatcact tcatcgccat tgcgcaccag
     3721 tgcggtaatc gccgcgtata acgcttccgt cgcccctgcc gttacggtga tatcgctatc
     3781 ggcatctggt tgatagccat acaaacgttc cgttttctga gcaatcgcct cgcgcaaggc
     3841 ctgcacgccg gtcatgggcg cgtattggtt tgccccctgg gcaacgtggt acgccagccg
     3901 ctcctgtaaa tagcgcggac catcaaaatc aggaaagcct tgcgacaggt taatcgcctg
     3961 gtgttgctgc gccagcgcgc tcatctgggt gaaaatagtg gtgccaagtt gtggaagttt
     4021 gctttgtgga atcagagggt tatttgtcat ttttattgta cctgacgaag ttgcgtgttg
     4081 aaggcactat aacacgatgc tagtatttgg caatcaagac gtttagatgt ctaaataaaa
     4141 caataaggac aacacaacat gcctcacaat cctatccgcg tggtcgtcgg cccggctaac
     4201 tacttttcac atccaggaag tttcaatcac ctgcacgatt ttttcactga tgaacaactt
     4261 tctcgcgcgg tgtggatcta cggcgtacgc gccattgctg cggcgcaaac caaacttccg
     4321 ccagcgtttg gacttccagg ggcaaagcat attttgtttc gtggtcattg cagcgaaagc
     4381 gatgtacagc aactggcggc tgagtccggt gacgatcgca gcgtggtgat tggcgtcggc
     4441 ggcggcgcac tactcgacac cgcgaaagcc ctcgcccgcc gtctcggtct gccatttgtt
     4501 gccgctccaa cgatcgccgc cacctgcgcc gcctggacac cgctctccgt ctggtataac
     4561 gatgccggac aggcgctgca ttatgagatt ttcgacgacg ccaattttat ggtgctggtg
     4621 gaaccggaga ttatcctcaa tgcgccgcaa caatatctgc tggcggggat cggtgatacg
     4681 ctggcgaaat ggtatgaagc ggtggtgctg gctccgcaac cagaaacgtt gccgctaacc
     4741 gtgcgactgg ggatcaataa tgctcaggcc attcgcgacg tcttgttaaa cagtagcgaa
     4801 caggcgcttg ccgatcagca aaatcagcag ttaacgcaat cattttgcga tgtggtggat
     4861 gccattattg ctggtggtgg gatggtcggt ggtctgggcg atcgttttac gcgtgtagcg
     4921 gcggcccatg ccgtacataa cggtctgact gtcctgccac aaaccgagaa gtttctccac
     4981 ggcaccaaag tcgcctacgg aattctggtg caaagcgcct tgctgggtca ggatgatgtg
     5041 ctggcgcaat taaccggggc gtatcagcgt tttcatctgc cgactacgct ggcggagctg
     5101 gaagtggata tcaataatca ggcggagatc gataaagtga ttgcccacac cttgcgcccg
     5161 gtggagtcca ttcattacct gccagtcacg ctgacaccag atacgttgcg tgcagcgttc
     5221 gaaaaagtgg aatcgtttaa agcctgacgt gcaaatttag cagcagcgcg cgccgccttt
     5281 tccaccgtag cgcgcgtcct gccgctcacg gaaaaactct tcgtaggtca tcggcgtttg
     5341 atcgggatgg ttaacccgca tatgttcgac atagttgtcg taatcaggca taccaatcat
     5401 cagcttcgcc gcctgaccta aatattttcc ggcttttgcc agtgaatcaa acatcttggc
     5461 ctccgctaac catgttgctg aaagagtgcc ggatgcggcg tgaacgcctt atccggccta
     5521 caaatgctat caacgcgctc ggtcagcccc ctcgccccat cggggagagg gttagggtga
     5581 gggggaaccc acggcgccat gccaacattc gccaacatcc cccctcactc tgactttagt
     5641 gcgcgccttt tgcctgcacc acgatctcct cgacattttc cggcattggc tcatacggcg
     5701 tttctttcgc cgttggcttc ggctctttca atgccgcaag tgccgtctta atcgagaaca
     5761 gtgccagaac caccacgacc accataaaga agatggttaa cccggcatcc agacggttgt
     5821 tgaacaccag ttgcgccagc tgtgactcag tatactgcga tggaatatta ccgctgtcga
     5881 tcattgcctg gaacttatta gcaatggcca ggaagccgac tttcgcatcc gggctaaacg
     5941 ctttctgcca gcctgcggtc agggtacaaa tcagcagcca ggccgttggt accagcgcca
     6001 cccaggcgta acgttgacgt ttcatcttga acaacaccac ggcacagagc atcagcgcca
     6061 tccctgccag catctggttg gcaataccaa acagcggcca cagagtgtta ataccgccca
     6121 gcggatcgac cacaccctga tgcaggaagt agccccacgc cagcacgcac agcgccgttg
     6181 ccagcaggtt agcaggcagt gaatcggtcc gtttcaggcc cggagaaacc acgcccagca
     6241 gatcctgcaa cataaagcgc gcagcacgcg tacctgcatc caccgccgtc agaataaaca
     6301 gagcttcaaa cagaatggcg aagtgatacc agaacgccac atccatcatg ccgcccagcg
     6361 cgccatgcag aatgtaggcc atccccaccg ccaacgtcgg cgcaccgccc gcacgggaaa
     6421 tgatcgactg ttctcccact tcgctggcaa tctggttgag cgtatccgga gtaatcgcaa
     6481 agccccagct actcaccact tgcgcggcag aagcgaccac atccgccgtc cctgccggag
     6541 ccagcaccgc catcgggctg ttcatggcga agtacacgcc cggatcgatg atacaggcgg
     6601 aaaccagtgc cataatcgcc acgaaggatt ccattaacat tccaccgtag ccgataaaac
     6661 aagcctgccc ttcgttcgcc agcatcttcg gcgtggtgcc ggaagagatc agcgcatgga
     6721 agccagacac cgcgccacag gcgatggtga taaacaggaa cgggaacagg ttaccggtcc
     6781 ataccgggcc agtgccgtca acaaatttgg tcaacgcagg catggtcagc gtcgggcgca
     6841 taatcaaaat gcctaccgcc agaccaacga tcgtcccgat tttcaggaag gtagagaggt
     6901 agtcacgcgg agccagcagc aaccataccg gcagcaccgc cgccacaaaa ccgtaaccca
     6961 ccagcatcca ggtcagctgc acgccggtaa agtcaaagta cggtgcccag gtcggacttt
     7021 ctgccaccca gccgccagaa ataatggcga aaatcaggaa taccagaccg atgaccgaca
     7081 cttcaccaat acgccccgga cgcagatagc gcaggtagat ccccatgaac agcgccagcg
     7141 gaatggtgaa cgcaacggta tacgttcccc acgggctatg agtcagggct ttcaccacga
     7201 tcatcgccag tactgcaagg ataatgacca tgatcataaa gcaggccacc agcgcgatca
     7261 ccccggcggt tggccccatc tcttctttga ccagctcacc cagcgaacga ccgtcacggc
     7321 gcgtagaaac aaacagcacc atgaaatcct gcaccgcacc ggcgagaacc accccggcga
     7381 gtagccagat catccctggc aggtaaccca tttgcgccgc cagtaccggc ccaaccaacg
     7441 gacctgctcc ggcaatggcc gcaaaatggt gaccgaacag cactttcttg tccgtcggca
     7501 catagtccag cccgtcgtta tggcgcaccg ctggcgtcat acgcgtcggg tcaaccgcca
     7561 gcacattttt ggcgatatac agaccataaa aacggtaagc gatcagatag atacagaccg
     7621 acgccaccac aatccacagc gcgttgatct gttccccacg atttaaagca atatatccca
     7681 gagcaaatgc tcccattaca gagagcactg tccagacgag gtatttccct gatttgttca
     7741 tagttgttat ccgtgtgcgt gtccatagag atgttacatt ttgtttctat aacatctttg
     7801 tacgatttaa caacacagca accatgtaaa tcggagttga aaccaatatt taacttaaca
     7861 atgttaactc gatcactccg ttaaccgagt tttcctgcaa tctcatataa ttctgtcggg
     7921 ttcatcccaa aactgccgta cccagccgac aagtgcagca acgccgcccc tgttcatcga
     7981 aaacgacgat ttcccagctc tgattttgcc gcccaagatg cagcggctgg cagacgccgc
     8041 gtaccttgcc ttcagacacc gggcgatgat gtgtggcgtt aagttccgtg cccaccacac
     8101 actgcccgtc gcgggtcatc ataaatccgg ccatcgatcc cagcgtttca gccagcgccg
     8161 ccgacgcgcc gccatgcagc aggccaaacg gctgatgagt acggatatca accggcattt
     8221 cggcttccag cacatcatcg cccagacggg tatacacaat tcccagatgc gccaccattg
     8281 tgttatcgct ggtggcgttc agttcgtcga gcgttaaatg gcgtttccag atcatgctta
     8341 tgcccccagc gttgagccgc catcgaccac aatatcctgt agggtgatat ggctggcgag
     8401 gtcagaggcg aggaacaaaa tcgtgttggc gatctcttgt ggacgggcga ttttccccag
     8461 cggaatgccg agtttaaact gctcgccaaa gccgcgaata cgctgttctt cggcgtcatc
     8521 gctcacccac agcgtgcgtt gcatatcggt atcggtggaa cccggcgaaa ccacattaca
     8581 gcgcacgccg ctacccgcca gttccagccc gacgctcaac gccaggcttt tcagcgccgc
     8641 tttcgatgcg ccataagcac tcataccaat acgcggcgtg tgtgcggcgt cggacgccac
     8701 agtgacaatc gccccgcccc gctgacggcg aaactggttc atggtttgct ggaacaggtt
     8761 aaacgcaccg ccgacattaa ccgcaaaagt ctgctgccag tcctccttac tgagctgatc
     8821 ggtcgcgccc atgcgcaaaa ttcccgccgc attgaccagc acgtccagtc gctccgtttc
     8881 agctaacagt cgctgacata cctgcgccac ctgcgcagca tcggcaacat ccattacttc
     8941 ggtcgcaaag ggatattgct cctgagcgaa cgcttgatca aaacctgtaa ctttcgctcc
     9001 cgcttcaaca aacgccagcg ccgtggcgta gccgatacct ttccccgcgc cggttaccca
     9061 gacattttta ccgctgaaat ccattatttc acctcgcggg agagtagctt ccaccaggcg
     9121 tcgatggtcg ggtttttcgc caacatgaca aagtcgatat cgccatgcac tttgcgccag
     9181 cgcgccgcca gcgccatcat gcgcaccgaa tccagaccgt agtcgatcag gttgtcgtca
     9241 tcgaacggtt catcggactc gtccagcaac ggcaggatca cctcacgcag cgccgctttg
     9301 ctggcgggga taggtgctgg cagtaattct tcggtcatca ccacccggcc agaacgtccg
     9361 gccacatatt tcagcgacat caaatgctcg tcacggctga aatcggccag cgcatccgcc
     9421 accataaacg gtttaatatc gcgcataaat gcgtcggttg cggtggtcat acagccaatg
     9481 tgggcatata ccccggtaat aatcagctgg ttacgtccac tctctttgag catttgctcc
     9541 agcggagaac gatgaaacgc gctgtagcgc cacttcacca gcaccgtgtc gtcggcatct
     9601 ggcgtcagtc gatccaccac cttttgctgt tccggcgaac gggtcaggcc cggcccccac
     9661 atatcattca acagcgcccg atcttcatcg ctctgctctt tcggctgggc ggtgtaataa
     9721 accgggatat tgtgctgttt gcagtagtcg cgcagcgcag caatattcgc gatcacctgc
     9781 tccatcatcg ggcagttctc gccccagaag ctgacaaaat agtcctgcat atcatggatt
     9841 aacaacgcgg cacgttgcgg ttcaaaggcc cagtcaactt tattctgcgg aatatcgtga
     9901 gactccggca gtgcgtaagc ctgtaatttt ggaatagcca tcgtgttctc tccttcaggc
     9961 tgatgcgcgt gacgccagcc actgacgtaa ttgtttttta tcgactttcc cgaccgccgt
    10021 cagcggaagt gaatccacac actccacgcg atccggtaat ttaaattcgg caataccctg
    10081 ttcacgcagg aaacgacgca cctgcaccgc gcgaagcggc tcttttacca ccagataagc
    10141 acagcttttt tcgcccatca gctcatcttc catactgacc agtgcggcgt agatcaccgc
    10201 cggatggcgc agtaacaggt tttcgatctc ttcggcagcg atcttctcgc cgccacggtt
    10261 gatctgatct ttctcgcgcc cctgcacggt gatgtaaccc tctggatcaa tagagatcag
    10321 atcgccggaa cagtaaaaac cgttggcatc aaaggcgctg gcattgtgct gcgggctttt
    10381 gtaatagcca cggaaggtat acggcccgcg cgtcatcagt cgtccgactt ccccttgcgg
    10441 cagtggattt ccttcggcat cggcaaccca tacttcgtca tccggacaca ttgggtaacc
    10501 ctgggtatgg ataattttct ccgcgctatc atcaagacgg gtatagttca ccagcccttc
    10561 cgccatacca aacacctgct gcaactggca accaatctca gcgggaatac gcgccgcaag
    10621 cgtggcagaa agacgtgcgc caccgacctg taacagtttc agcgaggcaa gctgcgcccg
    10681 gctttcgcct tcggtcagcg cctgcaacca caggctgact gccggcggca ccagcgcggt
    10741 aacgttaatc tgatgttttt caatcaatgg gaagcaaagc gtggcgctgg gatcggccgc
    10801 cagaacaacc gttcctccgg caagaaagac gcccagcgat cccggcgaac tcatggcgta
    10861 gttatgagcc gccgggatcg cgcacaggta gcgtgtctgt tgtgtgaact gacaaatctc
    10921 gacgctacga cgcacgctgt agtagtagtc gttatgagtg cgcgggatca gcttcggtgt
    10981 accggtggtg ccgccggaaa gctggaaata gaccacttca tcagcaggtg acggcgtagc
    11041 agtaaaaccg tctgccggat ggttaatcgc atcctgcaag ttatgctcac cgctgtcgtt
    11101 gagtagctgc accacgcgaa tggaagaatg ctctgcaaca aatgtgttga ggaaatcatc
    11161 cccgctaaac agcgcatgtt ggcgatcggc aatcagcaat gcgggttcaa tctggctggc
    11221 ataggcgttc agttcactac gctgatggct gaacaacgcc agcaccggcg caacgcccag
    11281 tttcagcagc gcgaaaaagg taatgtaaag ttcagcgacg ttacccagtt gtaccagcgc
    11341 ggtttcacca ggtttaatgc cctgacggcg taaactacac gcgaggttat ccgccgcctg
    11401 attcagctcc cggtaactca actgtcgctc gccgtcgata accgcgatgc tgtcactcgc
    11461 agcgtggcga gtcagaatgt cagtcagtgg caaatcctgc cagtagcctt tttcccgata
    11521 gcgacgggca aactcttccg gccagcgggt gaatggaatg ctcatcctcg ctccttaatg
    11581 caatccaaaa acgttcaaca tggtagaaag tttgacgcct gtttcgcgcc actcacccaa
    11641 cggtgacgac gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat tttcccgcag
    11701 cttcgcgcag cggatggtca ccacccattc gccgttacct tcgctgtcac accaacccac
    11761 aatgccgcca aacagttcgc ggtcgaacgg ctccagttca gcaataacct gggtcgcggc
    11821 ctgatgcggg aaaccgctca gcgcgggggt cggatgcagc agacaggcca gagtcagtgc
    11881 gttttcttgc gaattcgctt taccttcaaa gggagttgcg agatgccaca gcgtcggcgt
    11941 ggtaatcaat tgtggagagg aaggaacgtg taactcacta ctgcgttcgc gcagtacctc
    12001 tttcatcgcc tgagtcacca gttcatgttc atggcgatct ttttctgacg ccagcagacg
    12061 attacccgct tcgcgatcga gcacttcatc cggctgacga cgtgcggaac cggctaacgg
    12121 aatggagcta aaacgctcac cgtctttacg tagcagcagt tccgggctgg cccccagcag
    12181 aacgccacca tctgccagcg ggacatggaa gttgtaacta accgggtttt gcgcaatcaa
    12241 ccgttccagc aatacgccac tatcaatggc ggcgtcagtg gtgatatcaa tcaaccgtga
    12301 caacaccact ttgtcgacct gcggcgtggc ggtaagtgcg gcggcgcggg caaccatctg
    12361 ttcaaacgtg gtttgctccg gaattgcctg gcgttccacc acgttcagcg actggctgcg
    12421 ggtgaaacgg cgtgcggaag cttgtttttc ctgacgggag aacgactgcc aggattcagg
    12481 aatatacagc gacgaaggct gacgtggatc gaaaggaatc gccccgacca tcaccggatt
    12541 tttgatgccc tgcgctttgg catcggcaaa cagcgcggcg agtttttgct ggaagggact
    12601 gtcgggcgaa tccccgttca cagccggttc atcgaagcgg gcgaaacatc ctgacgtcgt
    12661 aaaactgcgg tacggcgaca taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg
    12721 ctgtacttcc tcagccagtg acgtatccat atcatcctcc acaaaatgat aaaggcttta
    12781 ataatgatta tcatttatat tttcggtcgc taacctaaaa gcaaacgcgc actatgtcaa
    12841 ctcttgaggt aacgcaatct gcaactcggg gttgcatctg gttgtggtta taatgaaaat
    12901 gagaagcatt aacttcatta ataacaggag gctgatttgt gagactcgcc ccgctctacc
    12961 gcaatgccct tctattaaca ggacttttgt tttcaggaat agccgcagtt caggccgccg
    13021 actggccgcg tcagattact gacagccgtg gcactcatac cctggaaagc cagccgcagc
    13081 gtattgtttc caccagcgtc accctgaccg gctcactgct ggcgattgat gctccggtga
    13141 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcgga tgaccagggc tttttacgcc
    13201 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagca
    13261 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    13321 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    13381 acgacaaaag ctggcaggcg ctgttaacgc aacttggcga aattaccggg catgagaaac
    13441 aagcggcaga gcggattgcg cagtttgata agcaactggc ggcggcgaaa gagcaaatca
    13501 aattaccgcc gcagccggtc actgccattg tctatactgc cgctgcacac agtgccaatc
    13561 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    13621 agttacccgc agggttaaat gccagccaaa gccagggtaa acgccatgac atcattcagc
    13681 ttggtgggga aaatctggct gcagggttaa atggcgagtc actattcctg ttcgccggtg
    13741 atcagaaaga tgccgatgct atttatgcta atccgctgct cgcgcacctg cctgcagtac
    13801 aaaacaagca ggtttatgcg ctgggaaccg agacgttccg tctggattac tacagcgcca
    13861 tgcaagtgct ggaacgactt aaggcgctgt tttaagcatt aaccgtcaga cgctgtcacc
    13921 tgcggcggcg tctggcgaaa acgtcgcaac tccaccagca ccagcaataa caacacgccg
    13981 ataatcaata aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat catcgctccc
    14041 aaaccaccca gcagcgccgc gcctatggca tcgcccgtca cgttttgtgc cgtccacaaa
    14101 ccgttaatcc gccctaacat cgcttccggg gtttgcgttt gcagcattgt gtattgcagc
    14161 aacgagctga ccgcactcaa ccagccgaac agcgccagac aaaccacgcc taaaatccac
    14221 atcggcatca ggccaaacag accaatggcg aggaacgatc ccagcgtgga gagcagcatc
    14281 aataaccctg gtcgcgcgct atgcgccagc ttcccgctgg ttaacgcgcc aatagccgcg
    14341 ccgagcggga tcgccgcata gagaaaacca atctgtgccg ccgacatctg ccagttgtca
    14401 gccagcgccg gatacagtac ccgcaccgca ctcgccatcg tcaataaacc acccagcagc
    14461 gcaatccctc ccaccagcgg gctggcgagt agaaaacgaa atcctgccag taatgatttc
    14521 aacggatgct cacgcggctg cggtggcggt ggcagtgccg gaaggcttaa caacggtagc
    14581 aaggtaataa acgtgcccgc cgccgccagc ccgtagttcc aggccacacc gccagtcgcc
    14641 agcagtaaac cgccaatcat gggcgaaatc accgacccca gacgcacggt caacatggtg
    14701 atcgccccgg cctgcattaa gttttcacgc cctaccagtg ctggtgtcgc cgccaatagc
    14761 gccgtaacgc caagcgatgc gaaaaaacca tcccataaac caagtaaata gattgccagc
    14821 aatgacggct ccggcagcag cgcgttcagg cacagtccaa tgaagccaat gccacaggtg
    14881 ccgcgcgcca gcaaaatcac ttttttgcgc tcatagcgat ccgccagcac accgccgacc
    14941 atcaggccaa caaacatcgc gccgccggtc agcgtcaccg aaagccccac ctgccaggtg
    15001 gaatgtgtca tcatctggat ctgcaccggc accgcgacgc cgagcaaacc cagagacaca
    15061 attgagatga aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt caacaggctg
    15121 aggttaagca gccaggattg tttattcatt acaatgcctt gccatcaaat attttaaata
    15181 cctattctta ggctgcacat gctaacatat ccaaataaga tcgataacga taattaattt
    15241 cattatcatg gaagttcgta tgtctggttc tgttgccgtg acacgcgcca ttgccgtgcc
    15301 cggattgctg ttattactga ttatcgcgac ggcattaagc ctgctcattg gggcaaaatc
    15361 actccccgct tccgtagtgc tggaggcctt ctccggcacc tgccagagcg ccgactgcac
    15421 catcgtgctc gacgcgcggc tgccgcgtac ccttgccggt ttactggcag gcggcgcgct
    15481 tggccttgcc ggggcgttaa tgcaaaccct cacccgaaac ccacttgccg accccggctt
    15541 gcttggcgtg aacgccggag ccagctttgc cattgtgctg ggtgcggcgc tgtttggtta
    15601 ctcttccgca caggaacaac tggcgatggc cttcgccggg gcgctggtgg cctcgttgat
    15661 tgttgccttt accggcagcc agggcggtgg gcagttaagt ccggtgcgtt taaccctggc
    15721 gggcgtggcg ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc tgcttaatcc
    15781 tgacgtctac gatcaactgc gtttctggca agccggttcg ctggatattc gcaatctaca
    15841 taccttaaaa gtggtgctga tcccagtgct gatcgccgga gcaactgcgc tattactgag
    15901 tcgcgcgctg aacagtttaa gccttggcag cgacaccgcg acggcgctgg gcagtcgcgt
    15961 ggcgcgcaca cagttgattg gtctgctggc gattaccgtg ctttgtggta gtgcgacggc
    16021 ggtagttggc cctattgcct ttattggcct gatgatgccg catatggcgc gttggctggt
    16081 gggtgccgat catcgctggt cgctgcccgt cacgctactt gctacccctg ccctgctgct
    16141 gtttgccgat atcatcgggc gggtgattgt gcccggtgaa ctgcgcgttt ctgtggtcag
    16201 tgcgtttatt ggtgcaccgg tgctgatctt cctcgtgcga cgtaaaacgc gaggtggtgc
    16261 atgatttacg tctctcgccg attactcatc acctgtttgc tgctgatttc cgcctgtgtg
    16321 gttgcaggta tctggggatt acgcagcggt gccgtcacgc tggaaatctc gcaggtattc
    16381 gccgcgctga tgggcgatgc gccgcgcagt atgacgatgg tggtcaccga atggcgttta
    16441 ccacgcgtgc tgatggcgct gttgattggc gcggcactgg gcgtcagcgg tgcgattttt
    16501 cagtcgctga tgcgtaaccc actcggcagc cctgacgtaa tgggctttaa caccggggcg
    16561 tggagcggcg tgttggtggc gatggtgctg tttggtcagg atctgacggc tatcgcgctg
    16621 gcagcaatgg tgggcggcat tatcacttcg ctgttggtct ggctgctcgc ctggcgtaac
    16681 ggtatcgaca cctttcggtt gattattatc ggtatcggcg ttcgcgccat gctggtggcc
    16741 tttaatacct ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc aggtttgtgg
    16801 aatgccggat cgctcaacgg cctgacgtgg gcaaaaacct cgccttccgc acccatcatt
    16861 atattgatgc taattgccgc cgccttactg gtacgccgga tgcgcttgct ggaaatgggc
    16921 gatgataccg cgtgtgcgct gggcgtcagc gtcgaacgtt cgcgtctgtt aatgatgctg
    16981 gttgcagtgg tgcttaccgc tgcggcaaca gcgcttgccg ggccgatttc ctttattgct
    17041 ttagtcgcgc cgcacattgc ccgacgcatt agcggcaccg ctcgctgggg gctaacccag
    17101 gcggcgctat gcggggcgct gttactgctg gcggccgatc tctgcgccca acaactgttt
    17161 atgccgtatc aacttccggt tggcgtcgtt accgtcagcc tcggcggtat ttaccttatc
    17221 gttttgttaa ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt ttgcgcggcg
    17281 aacagttaac cctgggatat ggcaaatata ccgttgcgga aaatctgact gtagaaatac
    17341 ctgatggtca cttcacggca attatcgggc caaatggctg cggtaaatcc acgttgctgc
    17401 gtaccttaag ccgcctgatg acgcctgctc atgggcatgt ctggctggat ggcgagcaca
    17461 ttcaacatta cgccagtaaa gaagttgccc ggcggattgg tctgttggcg caaaatgcca
    17521 ccacgctggg agatatcacc gtgcaggagc tggtggcgcg tgggcgttat ccacatcaac
    17581 cgctgtttac ccgttggcgc aaagaagatg aagaggcggt aacgaaagcg atgcaggcca
    17641 cgggaataac tcatcttgca gatcaaagcg tggataccct ttccggtggg caacgccagc
    17701 gagcgtggat cgcgatggtg ctggcccagg aaacggcaat tatgctgctc gacgaaccca
    17761 cgacctggct ggatatcagt catcagattg atttgctgga actgttaagc gaactgaacc
    17821 gcgagaaagg ctataccctg gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg
    17881 ccagccattt gattgcattg cgagaaggga aaattgttgc tcagggagcg ccgaaggaga
    17941 ttgtcactgc tgaactgatt gagcgcattt atggtctccg ctgcatgatc attgacgatc
    18001 cggtagccgg aacgccgctc gtggtgccgc tcggacgaac ggcaccttca accgcaaata
    18061 gttaaactaa gtggtctgcc atcatggcat cctgttttct ggatgccatc gcatagcgca
    18121 ataacacgct accacaagcc accatcccgc cgatcagcgc ggaaaggatc acaataatcg
    18181 ccttacccgg accgtctttt ttcactggca atgacgggct taactgatat ttaaacggcg
    18241 taaaattcac atcgttgata tttgcttttg ttaactgctc aacaagatac tgacgattac
    18301 gtaattcacc gttcagttcc gcaacgtcag tgaccgcttt ttcaatttcc agtttgcgtt
    18361 caataccgtc tgcgccgaga gaaatagaga aatcggggtc atctttaacg gcctgaccat
    18421 tactgtaaac gggcttttta attcctgccg cgttggcaat gtcgagtgaa taattgaggc
    18481 gctgaatgtt tgcatcaagt tgatttttca ttttaatgcg gtcctgagcc agtttttctt
    18541 tttcaaactg ggttttgatc tccagtttat tacggacgtt ttctatcgac tctttcacca
    18601 ccaacgcaga gatataatcg atatacccgc tcaaaacggt ctgcgcctct tcactggttg
    18661 gcgcggtaaa acttagcgtc caggaggtat acagtgacgg ttcatctttt ttcttactgg
    18721 cattgtcatc aaccgctttc attttttcgc tcaatgcgac aattgcgcga tgcaaatcca
    18781 gttcgtcgat tttcgcctct tttaattggt ccatcacata aggtgatgaa cgcaggtact
    18841 cttccagcaa gctaaccgac tgaaacttct tgataaacag gttaaatgct tctgtacgat
    18901 caattttgat atccagatcc agcacacgaa gcttagtgaa tgttttctcc aactcttgcc
    18961 actgaacagg ttctggaggc gtgacaaccg ccgcgctggt ccatttttgc ggcaggatga
    19021 aagagatcag caagcctgca caggcaaacg caaaaacgac cgccatgacc gtttttttgg
    19081 cccgccataa aactgagatt agattaagta aatcaatttc attattactg ggcgacgcca
    19141 gaggataatc gggaaaatga gcgtcacttc cctgtttaat attcagtgat gacatggggt
    19201 ataacctgaa aatgagtcca atagggggaa atctccacgg cgctcacttt accagttggt
    19261 gagaattatc cgaagctgaa gtttgtaaat tcattccact gaatgcaaca ttcactctat
    19321 ttaagaaaaa taaccagcaa acttataaat taaataaatg ataaacccat aattatttcc
    19381 tgcaattatg ggtttataga taatgttaat ttatctgttt agcgttgcgc gaataatcgg
    19441 cccaattttt tcaaatgccc ctggagagat aatatccaca tgcgcacaat cctgacgata
    19501 gatatccagc tccgcaatcc acggcgacca ggcgcgttcg gggctcatac cttcctgaag
    19561 cgtacgttca gcaacaaaca gcgtcgcttt tccgtcaaac ggtacgctat gagcagtcgt
    19621 cagcaggcgc acagcatcag cgtagttgcc ttcaatggtg gtaaacaact ccgttgaagt
    19681 acttccctgc tgtgccgcca ggaaggcctc gcgctcgcgg ttaatctccg ccagcacttc
    19741 cgggtccaga ccattagctt ctttttcctg ccagttttgc gtttctggcg gccaggtatc
    19801 cagcaagcca agaaatgcca cctgttcgcc acgggcacgt agtcgcgccg caatgccctg
    19861 cgccagtgta ccgccaaggg aataccccag caggtaataa gggccgtgcg gttgttgttc
    19921 aagtaacgtt gccagatgcg cttcgcagac ttcatccagg ttcgtcgctg tctgcatggg
    19981 gccatgaggg cgcggcgact gaatgccgat aatcgaccat agtggatcga gataacgcga
    20041 gagcacgcta aactgccagg caaaaccgga tgccggatgg aaacaaaaca gcgtcgggcc
    20101 attaccttca cgcaacggca gaatggtttc gaatcccatg cgccgggagc tgtcctcttc
    20161 accatcaata atcgttgcca gtttggcgac ggttgacgcg accatcacct gccccggcgt
    20221 cacctgacgg gcaaactgcc gacttaactg cgctgccagt ttcattgcca gtagcgaatg
    20281 accgccaagc gcgaagaaat cagcatcggc atcctgcacg tcacaaccca gcaacgacgc
    20341 gaatgccgca gcgataatcg tttcactgcc cgctttcggc gcacgccccg gcgtttgtgc
    20401 cttaagttca ggcaacggta aggctttgcg atccagcttg ccgttggcgc taagaggtaa
    20461 ctgtggaagt tgcagcagaa cgaccggcgc catatgcggc ggcaatgttt cgcgaagctg
    20521 tgcctgtaat gcgctggtat ccaacggcag acctgattgc gacaccagat agcccaccaa
    20581 ctgacgcgca tcaccaccgg tggctgccgc ctggttaatc acacaggcgt gggtaacggc
    20641 ttgttcgaca tccggcagcg cctgcatcac gcgatcgatt tcgcccagtt cgatacgctg
    20701 cccgcgaatt tttagctgat cgtcactgcg cccgaggtac tccaccgcgc cgttatccag
    20761 ccagcgggca acgtctccgg tacggtacat ccgttcacca gggacaaaag gatcggcaat
    20821 gaagcggctg gcggtcagat cgggtcgtcc aagatacccc tgcgccagtt gaataccggt
    20881 gagatagaga tctcccgcca cacccggcgg caccggatgc atcatcgcat cgagaatgcg
    20941 caagcccgta ttccacaccg gataaccaat cggcacactg ctgccgcgca cctgtgccag
    21001 ttcctcgcca aaagccggat accaactcac atctaccgcc gcttccgtcg ggccatatag
    21061 attatgcaac ggcgcgcccg ttaactgttg ccattcgcgg cataaatcag ccggtaaggc
    21121 ctcaccacta cagaaaacct gtttcaacgt cgcgcaattc tggcgagcgg tttgcggcgt
    21181 cagcgatgca acaaatgccg ccagcatcga cggcacaaag tgcgtggtcg ttacgccata
    21241 ttcggcaaag aattgctgca tagcgagcgg gtcgcggtgc gcttccggtt cagccatcac
    21301 cagtttagcc ccggcaataa acggccagaa aaactcccac accgagacat caaaactgca
    21361 cggcgttttt tgggcaacga catcttcacc tgtaagtgga taatgatttt gcatccacaa
    21421 caggcggtta acgatagccg tctgcccgac cattacccct ttcggcctgc cggtggagcc
    21481 agaggtaaag atgatataag ccgtgtgatg cggttgtgaa agttgcagcg gcgcactgcc
    21541 ctgcggtgta agcggggcgt tatagcaaag gttggttaaa tctggaacat cggcaaagcg
    21601 cggcagttga tcgtcggtgg tgattaacag cgacggacgc gcatcttcca gcatcatttt
    21661 caggcgatcg tccggataac cggtatccag cggtaaccag gccgcacctg cttcaacaat
    21721 cgcatgtagc gccagggtca aaaagaccga gcgcggtaat gccaccgcca cgctgtcccc
    21781 tggtttaacg ccgtgctcac gcagcagatt cgccagcgcc accacctgct cgcgcatttc
    21841 ccgatagctg aactggtaac gcgcatctgc cagcgccgga gcatccggtg tttttgccgc
    21901 ttgttctgcc accagcgcgc taagcgtggt ttctggaatc tcaacctgag tggcgttgat
    21961 ctgcgccagc tgcgcatact cacctggcag cataatatca acatcgccgc acaacagagc
    22021 cggatccgca gcgaattggg cgatcagcat tttcaggcgt tcagcatgct ggattaacgt
    22081 tggctcatcg taatgctgtt tattggcgag gatctcaata ctcaaatcac cgtgttcatc
    22141 cgggaacagg gccagttcaa ggtcattaac cggaccggtt gccagggtat gggtttgcgc
    22201 ctgaacacca ggaatatcca gttggtaatc aaataccttg atattgagta ccggaccaaa
    22261 cagcggttca tcacctgccg ctcgcccgct gtcacggaca atttgttcgg catcgtaacg
    22321 ttgatgacga cgcatttttt tcagttgtgc tgccagtcgg gttgccagtt ccggcagcgt
    22381 ttcttgtgcc gcaatgtgaa tacccaacgg taaaacgttg agcacgggtc cggtagccgt
    22441 cagcgccgcc gagcccagtc gacgcataaa gataaatccg gcggcgtagt ccatgcgatt
    22501 gcacaatcgc cccagccaaa aggctgccag cgcaagggct aaatcggtac gctgcacacc
    22561 tgaaagttgc gtagccagct ggcggaattc cccgtcggta aattccagtt tcaggcgtag
    22621 aatatctgcc gaagcgctgc gccccgctaa aggtgccgga gaaagtgacg cgggcggcgg
    22681 cagttgacga cgctgttccg cccagaatgc cgcatcacgc tgccaggctt cgctttcgcg
    22741 gtattgctgg tactcttcca ctacatcagc gaaaggcgta aacggcgaag caggcgtttg
    22801 ttcgccacgc agcaatgcgc agtaaatgtt ggcgatctgc cgagtaatgg ccgggaaact
    22861 gaagccatcg accagcaaat ggtgataacg ctgataccag taccagcggt tatctgccac
    22921 ctgtatcagc tggtgaaaga ccagtggttt accgctatcg acgcgcaaat cttgttgcaa
    22981 atccgcctgc attaatgcct gcgcagtacc gtgcggatca atattggttc gcaggtcgat
    23041 aatttctggc agttcgaata tcagcgcatc atcgacccat tgccagactt cgccgttatc
    23101 ttccgtaaaa cgcatccgca gcgtatctgc ttgcgctaat ccggcaacca ccgcgcgggc
    23161 cagtaatggc gcatcaacct ctccggttaa ctcaacgtaa tgcgccacgc tccaggcgga
    23221 gggtaattct gacagttttt ctgccatcca gatgccgggc tgtgcggcga ccaaaggtaa
    23281 atgctggctc attgtgcctc ctgcaactgg gtgaaattcg ccggtgtcag agtacgccag
    23341 tggacttcca gccactgctg gcaggactct tgtgactgcg gctgacacac aatgtcccag
    23401 cctgccggta aggcgcattg ttgcggccac agactgaatt gcccctgcgc attgcgcaaa
    23461 atgtaaaaca ctccctgtgg atcatcgaag ggattactga atgccatatt caactcctgt
    23521 catggaaaag tggttgccag aggtcgatta acccctgcat caagccaccg cgccaacaaa
    23581 gcgcatcatg tccgccgtca acctgacgcc agaaaatgga ttcttttata gggtgtaatt
    23641 gcgcatacag cgcctgattg gcccgcatga tcatcggctc gcgaataccc gcttccagca
    23701 caatgcgcag accttcggcg ctaacttcac cagcttttag cttttcaagt aacacgccct
    23761 cttgctgccc gccccgatgc ggccaccagt acgatcctga ctggcttaat acacagccaa
    23821 agcgttcagg ccagtgcagt ccggcataca gcgcggacag cccaccaaaa ctctgcccgg
    23881 cgaccacggt gcgatcggcg cgatcgctaa aaggggcaat agctttcacc aggggtaata
    23941 actcttgctg tactgccagc cagaaatccg cattacacgg cagttcgtgg gcgcggtgcg
    24001 tggtgtcgat agcgtcgatc aacacataca cggcgggagg aagttgctga cgatgggtca
    24061 gcgaaatcag cacaggccag acgggcatac tttgcgccca aaattcgcca tcgagcaaaa
    24121 ctgccagcgg gcgttcttct gctgttacat cgccggtggt aaaaatccat acacgacgtg
    24181 aatttttcaa ccgttcactt ttccagataa tttctttggc tggtatttct ggcgcttgcg
    24241 gacaatccca tcccggttgc agaggcgctt gcggcatttc gagtgcagaa acagcgtgcc
    24301 ctcgcccgcc tttccagctt tgtggattca gcggatcggc tattgcctgg ggtaatagtt
    24361 ttcgccagcc ttcacgtaat tcgaggcgat cgggggatgg tgcagaaaaa atgtcatcgc
    24421 gttcggtggg aataaagcag tagctgccgc gccagttggc attgagttgt gtcgtccact
    24481 gccagacatc agtacctgca attcgctgca tcgactgggg ctggctgttc tgatgatgat
    24541 cggtcacacc agtgatgtag atccatacgc gctttatcgt cgagtattct tcagaacctt
    24601 ggggatcacg ccaccagaaa gtgacctcaa acatttcgtc attcagacgc tgccattccg
    24661 ggccatgttt cgactgccac cagctctcac ttcctacttt taacgccgtc acaccataac
    24721 cccatgttta ctgtgcaatt tttcattgat tgcagaaata tattgataat attattgata
    24781 actatttgca tttgcaatag cgtaatggcg cgccgtggga agcgcggaca ttaattaacc
    24841 aactgcactg cgtgtctttc aggatcaaag gttttcgcgg tagcgggatg cgtcgtgttg
    24901 atgacgacca tgcccgacag ttgcaattcg tggcaaaaat gcaggaataa aacaatgaac
    24961 aagaagattc attccctggc cttgttggtc aatctgggga tttatggggt agcgcaggca
    25021 caagagccga ccgatactcc tgtttcacat gacgatacta ttgtcgttac cgccgccgag
    25081 cagaacttac aggcgcctgg cgtttcgacc atcaccgcag atgaaatccg caaaaacccc
    25141 gttgcccgcg atgtttcgga gatcatccgt accatgccag gcgttaacct gaccggtaac
    25201 tccaccagtg gtcagcgagg gaataaccga cagattgata ttcgcggtat gggtccggaa
    25261 aacacgctga ttttgattga cggcaagccg gtaagcagcc gtaactcggt gcgtcagggc
    25321 tggcgtggcg agcgcgatac ccgtggtgat acctcctggg tgccacctga aatgattgaa
    25381 cgtattgaag ttctgcgtgg tccggcagct gcgcgttatg gcaacggcgc ggcgggcggc
    25441 gtggttaaca tcattaccaa aaaaggcagc ggcgagtggc acggctcctg ggacgcatat
    25501 ttcaatgcgc cagaacataa agaggaaggt gccaccaaac gcactaactt tagcctgacc
    25561 ggtccgctgg gcgacgaatt cagcttccgc ttgtatggca acctcgacaa aacccaggct
    25621 gacgcgtggg atatcaacca gggtcatcag tccgcgcgtg ccggaacgta tgccacgacg
    25681 ttaccagccg ggcgcgaagg ggtgatcaat aaagatatta atggcgtggt gcgctgggac
    25741 ttcgcaccat tgcaatcgct ggaactggaa gcgggttaca gccgccaggg taacctgtat
    25801 gcgggcgata cccagaacac caactctgac gcttacactc gctcgaaata tggcgatgaa
    25861 accaaccgcc tgtatcgcca gaactactcg ctgacctgga acggtggctg ggataacggc
    25921 gtgaccacca gcaactgggt gcagtacgaa cacacccgta actcgcgtat tccggaaggt
    25981 ctggcgggcg gtaccgaagg gaaatttaac gaaaaagcga cacaggattt cgtcgataac
    26041 gatcttgatg acgtgatgct gcatagcgaa gttaacctgc cgattgattt cctcgttaac
    26101 cagacgctga cgctgggtac ggagtggaat cagcagcgga tgaaggactt aagttccaac
    26161 acccaggcac tgaccggaac gaataccggt ggcgctattg atggcgtgag tgccaccgac
    26221 cgtagcccgt attcaaaagc agaaattttc tcgctgtttg ccgaaaacaa catggagctg
    26281 actgacagca ccatcgtaac gccggggctg cgtttcgatc atcacagtat tgtcggcaat
    26341 aactggagcc cggcgctgaa catatcgcaa ggtttaggcg atgacttcac gctgaaaatg
    26401 ggcattgccc gcgcctataa agcgccgagc ctgtaccaga ctaacccgaa ctacattctc
    26461 tacagtaaag gtcagggctg ctatgccagc gcgggcggct gctatctgca aggtaacgat
    26521 gacctgaaag cagaaaccag catcaacaaa gagattggtc tggagttcaa acgcgacggc
    26581 tggctggcgg gcatcacctg gttccgtaac gattatcgca ataagattga agcaggctat
    26641 gttgctgtag ggcaaaacgc agtcggcacc gatctctatc agtgggataa cgtgccgaaa
    26701 gcggtggttg aaggtctgga aggatcgtta aacgtaccgg ttagcgaaac ggtgatgtgg
    26761 actaataaca tcacttatat gctgaagagt gaaaacaaaa ccacgggcga ccgtttgtcg
    26821 atcatcccgg agtatacgtt gaactcaacg ctgagctggc aggcacgaga agatttgtcg
    26881 atgcaaacga ccttcacctg gtacggtaag cagcagccga agaagtacaa ctataaaggt
    26941 cagccagcgg ttggaccgga aaccaaagaa attagtcctt acagcattgt tgggctgagc
    27001 gcgacgtggg atgtgacgaa gaatgtcagt ctgaccggcg gcgtggacaa tctgttcgac
    27061 aaacgtttgt ggcgtgcggg taatgcccag accacgggcg atttggcagg ggccaactat
    27121 atcgccggtg ccggggcgta tacctataac gagccgggac gtacgtggta tatgagcgta
    27181 aacactcact tctgatgcta acgtcagatt gttgacaaag tgcgcgtcgt tcatgccgga
    27241 tgcggcgtga acgccttatc cggcctacaa aaatcttgcc aattcaatat attgcaggac
    27301 catgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcatc agtctcgaat
    27361 atggtcgata tgaaaactac gcatacctcc ctcccctttg ccggacatac gctgcatttt
    27421 gttgagttcg atccggcgaa tttttgtgag caggatttac tctggctgcc gcactacgca
    27481 caactgcaac acgctggacg taaacgtaaa acagagcatt tagccggacg gatcgctgct
    27541 gtttatgcct tgcgggaata tggctataaa tgtgtgcccg caatcggcga gctacgccaa
    27601 ccggtgtggc ctgcggaggt atacggcagt attagccact gcggggctac ggcattggcc
    27661 gtggtttccc gtcaaccgat tggcgttgat atcgaagaaa ttttttctgc gcaaacagcg
    27721 accgaattga cagacaacat tattacacca gcagaacacg agcgactcgc agactgcggt
    27781 ttagcctttt ctctggcgct gacactggca ttttccgcca aagagagcgc atttaaggca
    27841 agtgagatcc aaactgatgc aggttttctg gactatcaga taattagctg gaataaacag
    27901 caggtcatca ttcatcgtga gaatgagatg tttgctgtgc actggcagat aaaagaaaaa
    27961 atagtcataa ctctgtgcca acacgattaa ttgacaacat ctggtacgat tcgcccgcag
    28021 ccatcactga ctgcgggcga aagtgtaaag caggtgcctt accactctga cctgacaacc
    28081 ggatatgcgg ggattgctcc ccgcacaacg gctacttctt cggttcgtaa gcgagaacag
    28141 ccttaaactc aatattacgt tcctttacgg tgaactcaca cagcgagtct ccgaccagaa
    28201 gcgtaaatcc cagcaccgtc aaacacagca ctatgactgc cacaagggca tatttcgtca
    28261 gcatgtttat attgcctcca tgcgaagaag ggactaccat ccgagttgtt ggggctcgga
    28321 gtggcagccc caggattgat agaaatatct cctggggctt catctttcca gacctcagag
    28381 ttaacctgaa accggaaagc ttcaggcacc cgccgttatc ttacccttcc ttagcaaaat
    28441 agctgtttat ttatacagtg aatcctgttt cggacttctg tcggcaaaac tcatcattgc
    28501 tcttaaaacg aaatcgcccg cagccatcac tgaccgcggg cgaaagtgta aagcaggtgc
    28561 cttaccatcc tgacctgaca accggatatg cggggattac tccccgcaca acggctactt
    28621 cttcggttcg taagcgagaa cagccctgaa ctcaatatta cgttccttca ccgtaaactc
    28681 acacagcgag tctccgacca gaagcgtaaa tcccggtacc gtcaaacaca gcactatgac
    28741 tgccacaagg gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aaggaactac
    28801 catccaaatt gtctggtttg gagtggcagc cccagggttg atagaaatat ctcctggagc
    28861 ttcatctttc cagacctcag agtaaacctg aaaccggaaa gcttcaggca cccgccgtta
    28921 tcttaccctt cctcaacaaa atagctgttt atttatacag taaatcctgt ttcggacttc
    28981 tcactcctca tttaatcaac tgaagctgag cccgtttttt ctgcatgcct caatcgtcaa
    29041 aattcttcgc caccagccag ccgtgccata acttgtcaca ttgcaaaagt agccaaattg
    29101 cttcagtcac catcaccaaa aaaagaaaca ttccagaact tccgcatctc gcccagccgc
    29161 tccgacttct atactgaata gaaaactcca acataagaga aacctatgcc attacccgat
    29221 tttcatgttt ctgaaccttt taccctcggt attgaactgg aaatgcaggt ggttaatccg
    29281 ccgggctatg acttaagcca ggactcttca atgctgattg acgcggttaa aaataagatc
    29341 acggccggag aggtaaagca cgatatcacc gaaagtatgc tggagctggc gacggatgtt
    29401 tgccgtgata tcaaccaggc tgccgggcaa ttttcagcga tgcagaaagt cgtattgcag
    29461 gcagccgcag accatcatct ggaaatttgc ggcggtggca cgcacccgtt tcagaaatgg
    29521 cagcgtcagg aggtatgcga caacgaacgc tatcaacgaa cgctggaaaa ctttggctat
    29581 ctcatccagc aggcgaccgt ttttggtcag catgtccatg ttggctgtgc cagtggcgat
    29641 gacgccattt atttgctgca cggcttgtca cggtttgtgc cgcactttat cgccctttcc
    29701 gccgcgtcgc catatatgca gggaacggat acgcgttttg cctcctcacg accgaatatt
    29761 ttttccgcct ttcctgataa tggcccgatg ccgtgggtca gtaactggca acaatttgaa
    29821 gccctgtttc gctgtctgag ttacaccacg atgatcgaca gcattaaaga tctgcactgg
    29881 gatattcgcc ccagtcctca ttttggcacg gtggaggttc gggtgatgga taccccgtta
    29941 acccttagcc acgcggtaaa tatggcggga ttaattcagg ccaccgccca ctggttactg
    30001 acagaacgcc cgttcaaaca taaggagaaa gattacctgc tgtataaatt caaccgtttc
    30061 caggcctgcc gctatgggct ggaaggcgtc attaccgatc cgtacactgg cgatcgtcga
    30121 ccactaacgg aagacacctt gcgattgctg gaaaaaatcg ccccttctgc acataaaatt
    30181 ggtgcatcga gcgcgattga ggccctgcat cgccaggtcg tcagcggtct gaatgaagcg
    30241 cagctgatgc gcgatttcgt cgccgatggc ggctcgctga ttgggctggt gaaaaagcat
    30301 tgtgagatct gggccggtga ctaagccggg aattgcctta cagaagaata tccctgacaa
    30361 tggtgtttta acttacattt aacaatgtta tgaaacaccc tttagaaacc ttgaccaccg
    30421 cagcaggcat tttgctgatg gctttcctct cttgcctgct gctgcccgcc cccgcactgg
    30481 ggcttacgct ggcacaaaaa ctggtgacca cgttccatct gatggatctt agtcagcttt
    30541 acactttatt gttttgtctg tggtttttag tgctgggcgc tattgagtat tttgttctgc
    30601 gctttatctg gcgacgctgg ttctcgctgg cggattaaac gtcgattatc gccgtacttc
    30661 gcggcatact ttgcttattc tccttcgcct aaaggaacgt ttatggataa gcaatcactg
    30721 cacgaaacgg cgaaacgcct ggcccttgag ttaccctttg tcgagctttg ctggcctttt
    30781 ggcccggagt tcgatgtttt taaaattggc ggcaagattt ttatgctgtc gtcggagcta
    30841 cgcggcgtcc cctttatcaa tctgaagtcc gatccacaaa aatccctgtt aaatcagcaa
    30901 atatacccaa gcattaagcc agggtatcac atgaataaaa agcactggat ttcggtgtat
    30961 cccggcgagg aaatctccga agcgttactt cgcgatctga tcaacgattc gtggaatctg
    31021 gtggttgatg gtctggctaa acgcgatcaa aaaagagtgc gtccaggcta aagcggaaat
    31081 ctatagcgca tttttctcgc ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca
    31141 aaattacttt cacatggagt ctttatggat atcatttctg tcgccttaaa gcgtcattcc
    31201 actaaggcat ttgatgccag caaaaaactt accccggaac aggccgagca gatcaaaact
    31261 ctcctgcaat acagcccatc cagcaccaac tcccagccgt ggcattttat tgttgccagc
    31321 acggaagaag gtaaagcgcg tgttgccaaa tccgctgccg gtaattatgt gttcaacgaa
    31381 cgtaaaatac ttgatgcctc gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat
    31441 gcctggctga agctggttgt tgaccaggaa gatgctgatg gccgctttgc cacgccggaa
    31501 gcgaaagccg cgaacgataa aggtcgcaag ttcttcgccg atatgcaccg taaagatctg
    31561 catgatgatg cagagtggat ggcaaaacag gtttatctca acgtcggtaa tttcctgctc
    31621 ggcgtggcgg ctctgggtct ggacgcggta cccatcgaag gttttgacgc cgccatcctc
    31681 gatgcagaat ttggtctgaa agagaaaggc tacaccagtc tggtggtagt tccggtgggt
    31741 catcacagcg ttgaagattt taacgctacg ctgccgaaat ctcgtctgcc gcaaaacatt
    31801 accttaaccg aagtgtaatt ctcccttgcc gggcatctgc ccggctattt cccctcagat
    31861 tctcctgatt tgcataaccc tgtttcagcc gtcatcatag gctgctgttg tataaaggag
    31921 acgttatgca ggatttaata tcccatgttg aagatttagc gggtattgag atcgatcaca
    31981 ccacctcgat ggtgatgatt ttcggtatta tttttctgac cgccgtcgtg gtgcatatta
    32041 ttttgcattg ggtggtactg cggaccttcg aaaaacgtgc catcgccagt tcacggcttt
    32101 ggttgcaaat cattacccag aataaactct tccaccgttt agcttttacc ctgcagggga
    32161 ttatcgtcaa tattcaggcg gtattctggc tgcaaaaagg caccgaagcg gcagatattc
    32221 tgactacctg cgcgcagttg tggatcatga tgtatgcgct gctttcagtc ttctcgctgc
    32281 tggatgttat tttgaatctg gcgcagaaat tcccggcagc atctcagtta ccgctgaaag
    32341 ggatatttca ggggattaaa ctgatcggcg cgattctggt cggcattttg atgatctcgc
    32401 tgctgattgg tcagtcgcca gcgattctga tcagcggtct tggtgcaatg gctgccgtgc
    32461 tgatgttggt atttaaagat ccgattcttg gtctggtggc aggtattcag ctttccgcga
    32521 acgatatgct gaaactgggc gactggctgg agatgccgaa atacggcgcg gatggcgcgg
    32581 tgatcgatat tgggttaacc accgtcaagg tgcgtaactg ggacaatacc attaccacta
    32641 ttcccacctg gtctctggtt tctgactcct ttaaaaactg gagcgggatg tcagcatctg
    32701 gcgggcgacg tattaagcgc agtatcagta ttgatgtcac cagtattcgt tttcttgatg
    32761 aagacgaaat gcaacgtctg aataaagcgc atttgttaaa gccttattta accagccgcc
    32821 atcaggaaat taatgagtgg aatcgccagc aaggttctac ggagtcggta ttaaacctgc
    32881 gccgaatgac caatattgga acctttcgtg cctatctgaa cgaatatctg cgtaaccatc
    32941 cgcggattcg taaagatatg accttaatgg tacgccaact ggctccaggt gataacggtt
    33001 taccgctcga gatctatgcg tttaccaaca ccgtggtgtg gctggaatat gaaagcattc
    33061 aggctgatat attcgatcac atatttgcga ttgtcgaaga gtttggtctg cgacttcatc
    33121 agtcgccaac cggcaatgat attcgctctc tggcgggtgc atttaagcag taattaaaaa
    33181 aaccgctctc atcgaatgga tgagagcgga catcgttttg tcggatgtag cgtgaatgcc
    33241 ttatttccga cgcagcgttt taaatgctac aaacaggaat acaatccaca ccggcagcag
    33301 gatcgctgac aagcgcatat catccatcgt gcacatcagc agcaaaatca tggcgaggaa
    33361 ggcgatgcaa agatagttgc cgaacggata gagcagcgcc ttaaactgtg tttcacgccc
    33421 ctgacgtcgc atcgctgcac gaaaacgcag atgcgccaga cagatcataa tccagttcaa
    33481 cagcagcgtt gctaccacca gcgccatcag cagaccaaac gctttttgcg gcagcagata
    33541 gttgattaac accaccagcg aagtgatcgc tccggaaagc atcagcgagt taatcggcac
    33601 gccgcgacgg ctaacacggg taaggaactt cggcgcatta ccctgcacag aaaggccaaa
    33661 cagcatgcgg ctgttagagt aaaccccgct gttatacact gacagcgatg ctaccagaat
    33721 gacgaagttc agcgcagaag ctaccacgtt gctgtcgaga ttatggaaaa tcatcacaaa
    33781 cgggctactg ttggatttca cttccaccca cggatagagc gccagtaaaa ccaccagtga
    33841 accgatgtaa aacagcagga tgcgatacac cacctgattt accgcttttg gaatgctttt
    33901 ttccggatcg cgcgcttcag cggcagtaat cccaatcagc tccagaccgc cgaaggagaa
    33961 cataattacc gccagcgaca aaatcagccc attccagccg gtggcgaaga aaccaccgta
    34021 gcgccagagg ttgtcgatac tggctttctc gccgccgtga ccagaaaaca gcagccacag
    34081 gccaaagccg atcataccga tgattgccag cactttaatc agcgcaaacc agaactcggt
    34141 ttcgccatat aagcgcacgt tcaccaggtt aacggcgttg atgataataa agaaggcggc
    34201 agcccaaatc cacgttggaa catccgggaa ccagtactgc atatagatgc ccgcagcggt
    34261 cagctctgcc attcccacca gcacgaacat cacccagtag ttccagccgg agaggaaccc
    34321 ggcgaacggt ccccagtatt tataggcaaa gtgggcaaat gaaccggata ccggctcttc
    34381 gaccaccatc tcgccgagct ggcgcataat caggaaagcg atgatcccgg cgacgccgta
    34441 gcccagcaat acagccggac ccgccatctg aatcgccggg ccaatgccaa gaaacagacc
    34501 agtaccaatt gcgccaccca gcgcaatcag ttgaatatga cggttatgta atccgcgatg
    34561 aagcgtcggc tcttgattcg acgcagtatc ttccgatacg tttgacgcgt ttttcacgcc
    34621 tttcccctgt gtgtcttttt tgttgagggg caccttttaa catttagtgc ccatcgtcgc
    34681 aagacacaat ccacacggtt aaaccgggta tcctgctttt atttccgtac ccgatgtcgg
    34741 tgcagccaca tcagcttata cgctgccggg ataataaaca gcgacagcaa aggcgcggtg
    34801 atcatgccgc caatcatcgg cgcggcaatc cggctcatca cctctgatcc tgcccccgtt
    34861 ccccacagaa tcggcagcag accagcgata atcaccgcca ccgtcatcgc tttcgggcgc
    34921 acgcgcagga ccgcgccgtg atgtaacgcc tcatccagct tctgctcgct gaatgtttgc
    34981 ggattattca acgacggctc ggcctctatg gcgtgacgta aatacatcag catcaccacg
    35041 ccaaattcgg cggcgacccc ggcaagggca ataaaaccgg tgcccgtcgc cacagaaaga
    35101 tgaaagccca tccaccacag caaccagatg ccgcccacca gcgcaaacgg tacgctgctg
    35161 ataatcagca acgcttcgcc cacccgacgg aacgccagat acaacagcac gaagatgatc
    35221 atcaacgtca tcggcaccat gagtttcagc ttatggttgg cgcgctccag tagctcgaac
    35281 tgcccggaga atgccacgct ggtgcccggt tttaactgca ctttttcagc tatcgctttt
    35341 tgcaaatcgt gaaccaccga caccatgtca cgatcgcggg catcgatata aatccagctc
    35401 gtcgggcgcg cattctcggt tttcagcatc gacggtccgg tagagacttt aacgtcggcc
    35461 acgtctgcca gggtgatttg ctgcttcatc ggcgtcagga tcggcaactg gcgcagtgcc
    35521 tgcggactat cgcgccagct ttgcggataa cgcagattaa ttggataacg ggcaatccct
    35581 tccaccgttt cgccaaccat cgccccgccc accgcagaag tcacaaacaa ctgcacatcc
    35641 gccaccgtca taccgtaacg tgcggctttt tcacggttaa tctcaacgtt gatatagcgc
    35701 ccaccttcca gccgctcggc aagcgcagaa gccacgcctg gcaccgttcg tgccacctct
    35761 tcaatttgct ccgccatcgc gtcgatatcc gccagcacag tgccggaaac ttttatgccg
    35821 atagggcttt taatgccggt tgagagcata tcgatacggt tacgaattgg cggcacccac
    35881 agattcgcca gccccggcag acgcacggtg ttatccagtt cctcaatgat tttgtccatt
    35941 gtcatgcctg gccgccactg ctcctgcggc ttaagctgga tggtcgtttc taccatctcc
    36001 agcggagcag aatcggtggc ggtttccgct ttcccggttt tgccaaatac ccgcgccact
    36061 tcaggtacgc tcataattag cttgtcggtt ttttgcagca tactcgccgc ctctgctgcg
    36121 gaaatccccg gcagcgtcga tggcatatac aacaagtcgc cttcattgat ctgcggtaaa
    36181 aattccccgc caactttatt gagcggccag agaaccgtca gcaccgaaag cgctgccacc
    36241 aacagcgtgg ttttcggcca gtgcagtact ttcagcaaca gcggatgata aacacgaatc
    36301 aaaaagcgat tgagcgggtt actgctttcc ggcggaattt tgccacggat ccagtaaccc
    36361 atcaggatag ggatcactac gatcgccagc agcgccgcac ccgccatcgc atacgttttg
    36421 gtgaacgcca gcgggccaaa cagacgccct tcctgccctt ccagggtgaa gatcgggata
    36481 aacgacagcg tgataatcag cagactgata aacagcgccg gccccacttc aacagatgca
    36541 ttggtaatca cctgccagcg cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc
    36601 cactcttcca gccgtttatg tgcattctcg atcatgacga tagcggcatc gaccatcgcc
    36661 ccgacggcaa tcgcaatgcc acccaacgac ataatattgg cattcagccc ctggaagtgc
    36721 atgacaataa aagcaataca caaccccagc ggcaacgaaa taatcgccac cagcgccgag
    36781 cgcacatgcc agagaaacag cgcacagact accgccacca caataaactc ttccagcaac
    36841 ttgccgctga ggttgtcgat ggcccggtca atgagctggc tgcgatcgta tgttgtaact
    36901 atctccacgc cttccggcag actgcttttc agcgtttcca gtttgtcctt cacggcggcg
    36961 atcacttcac gggcgttttt gccggagcgt aggatcacca ccccgcccac aacttcgccg
    37021 ttcagttcgg caatgccccg gcgcatctcc gggccaacct ggatcttcgc aacatcacgc
    37081 agataaacag gcacgccatt ttcactggct tttaaaacga tgtgattaaa gtcgtcgagc
    37141 gtttgtagat agccgctggc gcgcaccata tattctgctt ccgccagttc gattgacgaa
    37201 ccgcccgctt cctggtttga agcatccagc gcgcttttta cttcggcgag actgatgcca
    37261 tactgcgcca ggcgctgagg atcgataacc acctgatact ctttcaccac gccgcccacc
    37321 gacgccactt ccgcaacgtc agggatggtt tttagctcat atttgagaaa ccagtcctgc
    37381 aatgagcgta aatcggccag atcgtgctta ccgctgcgat ccaccagtgc atattcatag
    37441 atccagccaa cacccgtggc atctggtccc agctcggcgc tgactcccgc aggcagctta
    37501 ccctgtacct ggttgaggta ctccagcacc cgcgaacgcg cccagtacgg atcggtacca
    37561 tcttcgaaaa tgacatacac ataagagtcg ccaaactgcg agaaaccacg cacagtcttc
    37621 gcgccaggca ccgacaacat ggtggtagtt agcggataag tcacctgatt ttcaacgatt
    37681 tgcggtgcct gaccgggata gctggtttta ataatcacct gcacatcgga gagatccggc
    37741 aacgcatcca ctggcgtgtt aatgatggtc caggtgcccc agatactcag aaataacgcg
    37801 cccatcagca ccaaaaaacg gttcgccacc gagcgacgaa taatccattc aatcattggt
    37861 tattccctca atgcgcatgg gtagcacttt cagagcgcat ccgctccagt gcgccagaaa
    37921 tattggcttc ggaatcaatc aggaacaggc cgctggaaac caccttttca ccttccgcca
    37981 gaccagagcg taatgcggtg acaccttgcg atgcctggaa aacggcaacg cgttttggta
    38041 caaagcgccc gtcggcatca acggtaatca cccgctgttc gctgccggta tcaatcagcg
    38101 cctgtgacgg aatgagcagc atcggttcgc tggcggtatt gagttgcaac caggcgttca
    38161 ttcccggttt tagcgcctcg tcggcgttgt cgacttccag acgcagctgc agcgtgcggg
    38221 tcgcggcatc cacgccaggt agtagcgtcc atttgcggat ggtgagtgtt ttatcaggtc
    38281 gcgccggaac ggtgagcgta aactgcgagg catctttcac cagccaggcg atggactccg
    38341 ggatcgcagc agtgacccac accgggtcca taccctgaat tttcgctacc acgttatctt
    38401 tggcgatatt cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggtgctt
    38461 tgagcgtaaa gcgagtctgg attttttgcg tggcgatcag gcggcgaata tccgcctccg
    38521 gcattcccgc cagtcgcagc cgctcaagaa tgccttcggt ctgggtcgcc gtaccgccgg
    38581 tttcgcgcag cagtaaatac tcactctgcg cttccaccca gtcgggaatg gtcaggtcga
    38641 gaagcggcgc gcccttttgt actttatcgc ccacggtaag cggatacacc ttgtcgataa
    38701 acccggcggc gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgccg
    38761 ggaaactctg ggcaaaagtc agcggcccgc gcgtgacggt agccgttttc acccccagat
    38821 tctgcgtctg agtcgggtca atgcgcacac cagacgcaga actctcttca tcggcatatt
    38881 tcggcaccag atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg
    38941 gatacatcgg gtcgtaccag aataagattt tacgttctgc ggtcgacgtt ttttctgcag
    39001 gcggttccgc ctttgcaaac caggtaaaac ctgccgcaga aataataccg cccgcgatca
    39061 tgctgccgat aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt
    39121 taatatcctg taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc
    39181 cggttttaat tccactcatt ttcgtctgcg gggtgatggt aaagcgcatg gtcatctccg
    39241 gccagttcac ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt tccagatcaa
    39301 aaccttttac cacgccggtg gcactaatga cctgtggttg tgcttcgctc atggtttcat
    39361 gatgatgttc gttagcctgg gcattaaagc caataacggt aaacagactg aacattgcga
    39421 cttgcagtgc ttttttcatt tattttctcc tggagttaaa aattattgct gccaaccgcc
    39481 accaagtgcg gtatataaag aaatttcgtt gacctgacgg gcataattca ggtcaagtac
    39541 ggtttgtcgg gttgcaaata aagaacgctc ggcatccagc acttccagat aacttactgc
    39601 gccgtgctga tataatgccc gcgcccgttg caaagtaatt tgcagcgatg ccagataacg
    39661 ctgctgggcg ctaatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc
    39721 tttaaaggcg ttctggattt tctgttcata attcactacc gactgctgct ggcgaatttc
    39781 ggcgatatcc agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg
    39841 aataaaattc cacatcccgc tgctggcgtt aaataatgac gacagatcgc tgctggcggt
    39901 tgatattccg ctggtcaggc tgatagacgg gaaaaatgcc gcgcgtgcag caccaatatt
    39961 ggcattagcc gccattaacg catgttcagc ttccataata tccgggcgct gcaataagat
    40021 ttgcgacgac aagcccgccg gtaatttaac gctttgcagg ctgtcgctgt ttactgtctg
    40081 cgcttgcggc agcttgccgt agcttcccag taacagttgc aatgcattat tcgcctgcgc
    40141 cagttccccc tgacgtttag cgatgtcgct gcgggtactt tcaatcaccc cgcgagcctg
    40201 ttccagcgcc agaacattac tgctacctgt caacagttgt ttttcgacaa acgcatatga
    40261 ctgctgataa ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagctgctg
    40321 attgaaatag ctttgcgcga cattagaaac cagcagaata tgcaccgcgc gctgagcttc
    40381 ctcagtggct aaataatttt gtcgctcggc ttcgctcatg ttctttaagc gcccgaaaaa
    40441 atcgaggtca aagctggcgt taaggccagt agaaaactcc cgcgtcgtgg ctgaatcgcc
    40501 tttaagattg ccgctccagc tgccgctgcc ctcaccattg agctgtgggt agcggtcggc
    40561 atcagtcaga cgatattgcg cccgtgcctc ctgcactttc agcgtcgcca tgcgcaaatc
    40621 ccggttattc tccagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt
    40681 gcgccagccc gcgttctgat agttatctgc tgcgttaacc aggccgttct ggctgagtga
    40741 gaactgctgc ggtacgggca ttgccgggcg ctgataatcc ggtgccagtg aacaaccggt
    40801 tagcgcaagg gccacacaaa atggcagaag tttacaagga gacataggct cataatttct
    40861 ggtgatttta taccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg
    40921 acaaaattgt cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc
    40981 cgggcaacat gcggaggaaa tatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga
    41041 gaatacttga ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg
    41101 ctgaatggct accatctggc gatgaccgga gattatgacc tgataatcct cgatattatg
    41161 ctgccggacg tgaacggctg ggatatcgtg cgcatgttgc gctccgccaa taaagggatg
    41221 ccgattctgt tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg
    41281 ggggccgatg actatctggt gaagccgttc gcgtttgctg aactgctggc gcgggtgcgc
    41341 actctcctgc ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatttg
    41401 atggtcgatc tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt
    41461 aaagagttta ctctgctgga gttttttctc cgccatcagg gcgaagtgct gccccgctcg
    41521 cttatcgcct cgcaggtatg ggacatgaat tttgacagcg acactaacgc cattgatgtg
    41581 gcggtgaagc ggctacgcgg caaaatcgac aacgactttg agccgaagct gattcaaacc
    41641 gtgcgcggcg tgggttacat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc
    41701 gttttcgctg gcaacccgcc tgaccttttt tatcagcctg gccaccatcg cggcgttttt
    41761 cgcctttgca tggatcatga tccactcagt aaaagtgcat tttgccgagc aggatattaa
    41821 tgatttaaaa gagattagtg ccacccttga acgcgtacta aatcaccctg acgaaacgca
    41881 agcccgacgc ttaatgacgc tggaagatat cgtcagtggt tattccaatg tgttgatttc
    41941 cctggcagat agccacggta aaacggtgta tcactccccc ggtgcgcctg atatccgcga
    42001 gtttgcgcgc gatgccatac ccgataaaga cgcgcggggc ggcgaggtgt ttctcctctc
    42061 cggcccgacg atgatgatgc caggccacgg tcacgggcat atggaacaca gcaactggcg
    42121 gatgatcagt ttgccagttg gcccactggt tgacggcaaa ccgatttata cgctctacat
    42181 cgcgctttcg atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat
    42241 gaccgcatcg gtcatcagca tcctgatcgt ctttatcgtg ctattggcgg ttcataaagg
    42301 tcacgcgccg atccgcagcg tcagccgtca aatccagaat atcacctcga aagatctcga
    42361 cgtccgcctt gacccgcaga ccgtgccgat tgagctggaa cagctggtac tgtcgttcaa
    42421 ccatatgatc gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggatat
    42481 cgcccacgaa attcgcacac caattactaa tctcataacg caaacggaaa tcgccctcag
    42541 ccagtcgcgc agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac
    42601 gcgaatggcg aaaatggtca gcgatatgct gtttctcgcc caggccgata acaaccagtt
    42661 aatccccgaa aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt
    42721 cgaggcttta gcggaagatc gcggcgtgga gctgcagttt gttggcgacg agtgccaggt
    42781 tgcgggcgat ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcga acgccctgcg
    42841 ttatacgcca cccggagagg ctattgtagt gcgctgccag acggtcgatc atctggtgca
    42901 agttatcgtc gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga
    42961 ccgtttctac cgcgttgacc cctcccgcca acgaaaaggc gaaggtagcg gcattgggct
    43021 ggcgatagtg aaatcaattg ttgtcgcgca taaaggcacg gttgcggtta cgtcaaatgc
    43081 gcgggggaca aggtttgtga ttgtgttgcc ggagaggggg tgattattaa gttcaaattc
    43141 agggcgaggg tttacggcgt ttttcgctaa atttccatca ttataaacaa gccacctatc
    43201 atataaagca atgaataatt aaatcgcgat taatcatcat taatttttat attattcacc
    43261 caatccattt ataaagtgaa tttgataaat tcctaattta aaatatccac tattgagcca
    43321 gtatttaaca acgttaagat gtgcaatgtg cagatcatgt tatcagggtg gagcggtagc
    43381 atatattttt taagcaatca ttcattttaa tgatgttaat tgggttattt agttgtgaat
    43441 aaaaacatat tatgaatatt ggtttaactt tgcgtttttg ttactcgctg tgatgtgcgc
    43501 acctgtcctg aataacgtca ggaaacaata agttgagctt gtgattattt ttttgactga
    43561 acagacaccc gtttctgtaa agtaatattt aaacaaaatc ctcacataat catagcattt
    43621 tttgttcctt ccctaataac gtcttaagtc gtcccgctta tctcattatc cgcagttgta
    43681 cggtgcggat tattattcat tttaccggag gttgctatgt caaccggatt acgtttcaca
    43741 ctggaagtgg acggcctgcc accggacgct tttgcggtgg tttcctttca tctcacccag
    43801 tcactctctt cgctcttttc cctcgatctc tccttggtca gccagcagtt tctctccctt
    43861 gaatttgcgc aggtgctgga caaaatggcc tacctgacaa tatggcaggg tgatgatgta
    43921 cagcgccggg tgaaaggtgt ggtgacctgg tttgaactgg gggagaacga caaaaaccag
    43981 atgctgtaca gcatgaaggt gcacccgccg ctgtggcgtg ccggtctgcg ccagaacttc
    44041 cgtatcttcc agaacgagga catcaaaagc atcctcggca cgatattgca ggaaaacggg
    44101 gtgaccgagt ggagtccgct gttcagtgaa ccgcatcctt cccgtgagtt ttgtgtccag
    44161 tacggtgaga ctgattacga tttcctgtgc cggatggcgg cggaggaagg catcttcttt
    44221 tatgaggagc atgcttacaa aagtaccgac cagagcctgg tgctgtgcga cacggtccgc
    44281 catctgcccg aatcttttga aatcccctgg aacccgaaca cccgtaccga ggtgagcacc
    44341 ctctgcatca gccagtttcg ctacagcgca caaatccgcc cttcttccgt ggtgaccaaa
    44401 gactacacct ttaaacgccc cggctgggca ggacgttttg atcaggaagg ccagcaccag
    44461 gattaccagc gcacgcagta tgaggtgtat gactaccccg gacgtttcaa gggcgcccac
    44521 gggcagaact ttgcccgctg gcagatggag ggctggcgaa acaatgcaga aaccgcgcgg
    44581 ggaatgagtc gctcgccgga gatatggccg ggacggcgaa ttgtgctgac ggggcatccg
    44641 caggcgaacc tgaaccggga atggcaggtg gtggcaagtg aactgcacgg cgagcagccg
    44701 caggcggtgc cgggaaggcg gggagcaggg acggcgctgg agaaccattt tgcggtgatc
    44761 ccggcagacc ggacatggcg accccagccg cggctaaaac cgctggtgga cggtccgcag
    44821 agcgccgtcg tgacggggcc ggagggtgag gaaatcttct gtgatgaaca tggccgagtg
    44881 cgggtgaaat tcaactggga ccgttataac ccggcagacc aggacagctc gtgctggatc
    44941 cgtgtggcac aggcgtgggc aggcaccggt tttggcaacc tggcgatacc gcgtgtgggt
    45001 caggaggtga tagtggactt cctcaacggc gatccggacc agccgatcat tatggggcgc
    45061 acctaccacc aggaaaaccg cacccccggc agcctgccgg ggacgaagac gcagatgacc
    45121 atccgttcca aaacgtatat gggcagcggg tttaatgagc tgaagtttga tgatgcgaca
    45181 gggagagaac aggtctacat ccacgcgcag aagaacatgg ataccgaagt gctcaacgac
    45241 cgtaccacca ccgtaaaaca cgatcatcgc gaaaccgtaa aaaatgacca gacggtcacg
    45301 atccaggaag gtaaccgcct tcttacggtg gaaaaaggcc acaagatcac cggagtactg
    45361 aaagggtctt tatctgagga tgtctttcag gacagaggca cgattgccgg ttcggtgcat
    45421 gttgacgctg taaacaatgg tggcgaaggc aacggtatac aggcttatac ggcaattaag
    45481 gaaattatgc tggccgtgga ggaaagcaaa attgcgctga cgccggatgg cattcagcta
    45541 caggtcgggg aatcaacggt aatcaggctg tcgaaggatg gcatcaccat cgtgggcggt
    45601 tctgttttca tcaactgatt cactcagatt aacagggagt attttatgca gttcactttt
    45661 aatgaaggtc atattcaact gccatcgcaa tggcaggatc agtcgatgca ggtcctggta
    45721 tccacggata acagtggcat caacctggtc attacccgcg accccgtttc acagggaacc
    45781 ctgacaccag agctgtacca ggaaacgctg gcgctgtacc agggcaaact tgatgggtac
    45841 accgaacatg cctgccggga aatcactctg gcagaggccc cggcctggct gctggattat
    45901 tcctggaacg gtccggaaga tgaagggaat caggggcgga ttagccagat agccgtattc
    45961 cagcggcgtg gagatacgct gctgaccttc actttcagca cctccttatc cctgaagaac
    46021 agtcaaaaga cgatgctgct ggaggtgata aagagcttca ccccgttgcc accggagaat
    46081 gacatccaaa aggaccagcc gcgctgacat aaacgaggga taaaacatga gtgaaggacc
    46141 aggcgggcca cagggagcga ccgcaggcgg tacgctggca atgcggatgc tgtcgcagcg
    46201 ggcgatggcc gccagccaga tgaaacgggc agccaacgac aaagccattg cacagatgtt
    46261 ggcatcaaaa aagtctggcc cccccgccgc caggctgggc gatgaaattc agcacaagag
    46321 ttttttgggg gcgctggcag gggccgtgct gggggcgata gtgaccatag cagaaggttg
    46381 cctgattatg gccgcctgtg ccaccggccc ttatgcgctg gttctggtgc ctgcgctgat
    46441 gtatgccagc tataaggcga gtgattatgt ggaggagaaa cagaaccagc ttgaatcatg
    46501 gataaacagc ttttgtgaca cggacggcgc catcaatacc ggttctaaaa atgtaaaaat
    46561 taacggaaag ccagcagccc gtgcagccgt cacccttccc cctcctcccc cacctggagc
    46621 aatacctgaa gtcccacagg gggaaccctc atggggtgat attgccactg acctgcttga
    46681 atcggcagcg gaaaaagcag taccactggc gaaggcctgg gggaacgctg ttatcaccct
    46741 gacggacagc aatgccggtt ttatggatcg cgtcagcgcc ggcgcatcgc ttctgtttcc
    46801 cgccggtccg gtattaatgg agtttgccac catggtgggc gggcgtggcg aaatcaaaaa
    46861 agatgtggat ttcccggaag ccggtgagga cacggcgctc tgcgacaagg agaacaaacc
    46921 accgaggata gcccagggca gcagcaacgt ctttatcaac aatcagcctg ccgcgcgcaa
    46981 gggcgacaaa ctggagtgca gcgcggcaat cgtggaaggt tcgccggacg tctttattgg
    47041 gggtgagcag gtcacctatc tggatatcca gccggagttc ccgccatggc agagaatgat
    47101 cctgggagga ataacgatag ccagctatct tctgccgcca gcaggactgc tgggaaaact
    47161 ggggaatctg gcgaaactgg gcaaactggg aaacctgctg gggaaaagcg gtaagctgct
    47221 gggcgcaaag ctcggcgcgt tgctggggaa aacaggtaat tcgttaaaaa gtattgccaa
    47281 taaagtcatc agatgggtaa cagatcctgt cgatccggta accagcgcat actgcgacga
    47341 acgtaccgac tttacgctgg gccagacact ccccctctcc ttcacccgtt tccacagttc
    47401 tgtactgccg ctgcatggcc tgacgggcgt gggctggagc gactcctgga gcgaatacgc
    47461 ctgggtgcgt gaacagggaa accgggtgga tgtcatcagc ctgggagcca cgctgaactt
    47521 cgccttcgac ggtgaaagtg atacggcggt taacccgtat cacgcccagt acattctgcg
    47581 ccgccgtgat gattatctgg agctgttcga cagggatgca ctgagcagcc gcttctttta
    47641 tgacgccttt ccgggaatgc gtctgcgcca cccggtgact gacgatacca gcgatgaccg
    47701 cctggcacac agccccgcag accggatgta catgctgggc gggatgagcg acaccgccag
    47761 caaccgcatc acgtttgagc gcgacaccca gtaccggatc acgggtgtca gtcacaccga
    47821 cgggatccgg cttaaactga cgtaccacgc cagcggctac ctgaaagcca ttcaccgcac
    47881 ggataacggc atacagacgc tggcgaccta cgaacaggat gcgcggctgg actaccacct
    47941 gttttatgag tacgacgctg cggaccggat catccgctgg tccgataacg accagacgtg
    48001 gagccgtttc acctacgatg cacagggccg gtgcgtgacc gtcaccgggg cggagggcta
    48061 ttacaacgcc acgctggact atggtgacgg ctgcaccacc gtgacggacg gcaagggcat
    48121 tcaccgttat tactatgatc ctgacggcaa tattctgcgg gaagaagcgc cggacggcag
    48181 caccaccacg tatgaatggg atgaattcca tcacctgctg gcccgccact cccctgccgg
    48241 gcgggtggag aagtttgaat acaacgccgc acacggtcag ttaagccgtt acacggcggc
    48301 agacggcgcg gattggcagt actgctatga tgagcgcggc ctgctcagca acatcaccgc
    48361 ccctgccggg cagacgtgga cgcagcagtg tgatgaacgc ggcctgccgg tgagtctggt
    48421 atcgccacag ggcgaagaga cccggctggc gtacacccct caggggctgc tgtcggggat
    48481 attccgccag gatgaacggc gtctgggcat agagtacgac caccacaact ggccggaaac
    48541 actcaccgac gtgatgggcc gcgaacacca caccgaatac agcggtcacg acctgccggt
    48601 gaagatgcgc ggccccggcg gtcagtcagt gcggttgcag tggcagcagc accataaact
    48661 gagtggcctt gagcgggcag gaaccggcgc ggaaggattc cgctacgacc gccacggcaa
    48721 cctgctggcg tacacggacg gtaacggcgt tgtctggaca atggagtacg gcccgtttga
    48781 tttgccggtg gcgcgaacgg acggtgaagg ccaccgctgg cagtaccgct acgataaaga
    48841 cacgctgcaa ctgacagaag tcattaaccc gcagggcgag tcttatcttt atattctgga
    48901 caactgtggc cgggtgacgg aagaacgtga ctggggcggc gtggtctgtc gttaccgtta
    48961 tgacgctgat ggcctgtgta ccgccagggt caacggcctg gaggaaacca tcctctacag
    49021 ccgggatgcc gcaggccgcc tggcagaagt catcactccg gaaggcaaaa cgcagtatgc
    49081 gtatgacaaa tccggcaggc tgacgggtat cttcagcccg gacggcacat cacagcgcac
    49141 cggctatgat gaacgcgggc gggtgaatgt caccactcag ggccgacggg ccattgaata
    49201 ccactacccc gacgaacaca ccgtcatccg ctgtatcctg ccaccggaag atgaacgcga
    49261 cagacacccc gacggatccc tgctgaaaac cacataccgc tacaacgccg ccggagaact
    49321 gacggaggtt atcctgccgg gggatgagac gctgacgttc agccgtgatg aggcgggacg
    49381 tgaagtgctc cggcacagta accggggttt tgcctgtgaa cagggctgga atgcagccgg
    49441 tcagcctgtc agccagcgcg ccggactttt cccggcggaa gccacatggg gcggactgct
    49501 cccttcactg ctacgggaat accgttacga cagcgcgggt aacgtatcag gcgtcaccag
    49561 ccgggaagat tacggacggg aaacacaccg ggagtaccgg cttgaccgga acggccaggt
    49621 cacggcggtg acagcctcag gcaccgggct gggctatggc gaaggcgacg agacttatgg
    49681 ctatgacagc tgcggctacc tgaaggcgca gtctgcgggc agacaccgga taagcggaga
    49741 gactgaccag tatgccgcag gccaccggct gaaacaggcc ggaaacacac agtatgacta
    49801 tgacgccgca ggccggatgg tcagccgcac aaaacaccgt gacggctacc gcccagaaac
    49861 agagcggttc cggtgggaca gccgggacca gctgaccggg tatcgcagcg cacaggggga
    49921 gcagtgggaa taccgccacg acgccagcgg cagacggacg gaaaaacgct gcgaccggaa
    49981 gaaaatccgt tttacgtacc tgtgggacgg cgacagtatt gcggaaatcc gggaataccg
    50041 cgatgataaa ctgtacagcg taaggcacct ggtgtttaac ggctttgagc tgataagcca
    50101 gcagttcagc cgggtacgac agccgcatcc gtccgtggcc ccgcagtggg tgacgcgaac
    50161 gaatcatgcg gtgagcgacc tgacgggccg cccgctgatg ctctttaaca gtgaaggtaa
    50221 aaccgtctgg cggccggggc agaccagcct gtgggggctg gcactcagcc tgcccgcaga
    50281 cacagactac ccggccccgc gcggggagcg ggacccggaa gcggaccccg gcctgctgta
    50341 tgcgggacag tggcaggatg cagaatcggg gctgtgctat aaccggttcc ggtactacga
    50401 gccggaaacc gggatgtacc tggtgagtga tccgctgggg ttgcagggag gggagcagac
    50461 ttatcggtat gtgccgaatc cttgtgggta tatcgatcct ttggggctgg ctatatgtca
    50521 gttagcccgc tggacgaaat gggggagtga gcaaagcaac atatctgatg ttttgaactc
    50581 attagggaat agagcactta aatatgctaa tggtgattgg ataaaatcag aggctgcatt
    50641 caataaatac ataaacatga taaataaaag actagaatta acaggtagta aatttagagt
    50701 tgagattcaa ccagccataa aaaatggaga gcgagttcct gcgacaacga atggaccatt
    50761 taaagtaaat ggtaagtgga catccggcac tcattataca ggtggttcca aacgtctaga
    50821 tgccggtatt attga
//
LOCUS       DABSYT020000023        49185 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.023, whole genome shotgun sequence.
ACCESSION   DABSYT020000023 DABSYT020000000
VERSION     DABSYT020000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 49185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.023"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..475
                     /locus_tag="ID725_RS08655"
                     /old_locus_tag="pgaptmp_001731"
     CDS             <1..475
                     /locus_tag="ID725_RS08655"
                     /old_locus_tag="pgaptmp_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGV7850353.1"
                     /translation="HFFNVDMIRVNFSKCLFHDCSMHGVKIKPWLPVKWTKELINDYL
                     YGCLLSLYSICARDIYNMNAGNNVKVAADAFLEILYSLKNKYCIKLLSAQDRAFIYEF
                     ARMIFAYINDKSIEILLLSCFAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
     gene            686..1009
                     /gene="ybjQ"
                     /locus_tag="ID725_RS08660"
                     /old_locus_tag="pgaptmp_001732"
     CDS             686..1009
                     /gene="ybjQ"
                     /locus_tag="ID725_RS08660"
                     /old_locus_tag="pgaptmp_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGV7850354.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            1006..1836
                     /gene="amiD"
                     /locus_tag="ID725_RS08665"
                     /old_locus_tag="pgaptmp_001733"
     CDS             1006..1836
                     /gene="amiD"
                     /locus_tag="ID725_RS08665"
                     /old_locus_tag="pgaptmp_001733"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGV7850355.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1833..2846)
                     /gene="ybjS"
                     /locus_tag="ID725_RS08670"
                     /old_locus_tag="pgaptmp_001734"
     CDS             complement(1833..2846)
                     /gene="ybjS"
                     /locus_tag="ID725_RS08670"
                     /old_locus_tag="pgaptmp_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGV7850356.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2945..4375)
                     /gene="ybjT"
                     /locus_tag="ID725_RS08675"
                     /old_locus_tag="pgaptmp_001735"
     CDS             complement(2945..4375)
                     /gene="ybjT"
                     /locus_tag="ID725_RS08675"
                     /old_locus_tag="pgaptmp_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGV7850357.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(4386..5387)
                     /gene="ltaE"
                     /locus_tag="ID725_RS08680"
                     /old_locus_tag="pgaptmp_001736"
     CDS             complement(4386..5387)
                     /gene="ltaE"
                     /locus_tag="ID725_RS08680"
                     /old_locus_tag="pgaptmp_001736"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGV7850358.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5424..7142)
                     /gene="poxB"
                     /locus_tag="ID725_RS08685"
                     /old_locus_tag="pgaptmp_001737"
     CDS             complement(5424..7142)
                     /gene="poxB"
                     /locus_tag="ID725_RS08685"
                     /old_locus_tag="pgaptmp_001737"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGV7850359.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7275..8243)
                     /gene="hcr"
                     /locus_tag="ID725_RS08690"
                     /old_locus_tag="pgaptmp_001738"
     CDS             complement(7275..8243)
                     /gene="hcr"
                     /locus_tag="ID725_RS08690"
                     /old_locus_tag="pgaptmp_001738"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGV7850360.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8255..9907)
                     /gene="hcp"
                     /locus_tag="ID725_RS08695"
                     /old_locus_tag="pgaptmp_001739"
                     /gene_synonym="priS"
     CDS             complement(8255..9907)
                     /gene="hcp"
                     /locus_tag="ID725_RS08695"
                     /old_locus_tag="pgaptmp_001739"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGV7850361.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(10051..10950)
                     /gene="lysO"
                     /locus_tag="ID725_RS08700"
                     /old_locus_tag="pgaptmp_001740"
     CDS             complement(10051..10950)
                     /gene="lysO"
                     /locus_tag="ID725_RS08700"
                     /old_locus_tag="pgaptmp_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGV7850362.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11408..12103)
                     /gene="aqpZ"
                     /locus_tag="ID725_RS08705"
                     /old_locus_tag="pgaptmp_001741"
     CDS             complement(11408..12103)
                     /gene="aqpZ"
                     /locus_tag="ID725_RS08705"
                     /old_locus_tag="pgaptmp_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGV7850363.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            12529..14187
                     /gene="ybjD"
                     /locus_tag="ID725_RS08710"
                     /old_locus_tag="pgaptmp_001742"
     CDS             12529..14187
                     /gene="ybjD"
                     /locus_tag="ID725_RS08710"
                     /old_locus_tag="pgaptmp_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGV7850364.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGHAD"
     gene            complement(14184..15140)
                     /gene="ybjX"
                     /locus_tag="ID725_RS08715"
                     /old_locus_tag="pgaptmp_001743"
     CDS             complement(14184..15140)
                     /gene="ybjX"
                     /locus_tag="ID725_RS08715"
                     /old_locus_tag="pgaptmp_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGV7850365.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            15291..16406
                     /gene="macA"
                     /locus_tag="ID725_RS08720"
                     /old_locus_tag="pgaptmp_001744"
     CDS             15291..16406
                     /gene="macA"
                     /locus_tag="ID725_RS08720"
                     /old_locus_tag="pgaptmp_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGV7850366.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            16403..18349
                     /gene="macB"
                     /locus_tag="ID725_RS08725"
                     /old_locus_tag="pgaptmp_001745"
     CDS             16403..18349
                     /gene="macB"
                     /locus_tag="ID725_RS08725"
                     /old_locus_tag="pgaptmp_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGV7850367.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(18422..18646)
                     /gene="cspD"
                     /locus_tag="ID725_RS08730"
                     /old_locus_tag="pgaptmp_001746"
     CDS             complement(18422..18646)
                     /gene="cspD"
                     /locus_tag="ID725_RS08730"
                     /old_locus_tag="pgaptmp_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGV7850368.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            18796..18870
                     /gene="yljB"
                     /locus_tag="ID725_RS08735"
                     /old_locus_tag="pgaptmp_001747"
     CDS             18796..18870
                     /gene="yljB"
                     /locus_tag="ID725_RS08735"
                     /old_locus_tag="pgaptmp_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGV7850369.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            18969..19289
                     /gene="clpS"
                     /locus_tag="ID725_RS08740"
                     /old_locus_tag="pgaptmp_001748"
     CDS             18969..19289
                     /gene="clpS"
                     /locus_tag="ID725_RS08740"
                     /old_locus_tag="pgaptmp_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGV7850370.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            19320..21596
                     /gene="clpA"
                     /locus_tag="ID725_RS08745"
                     /old_locus_tag="pgaptmp_001749"
     CDS             19320..21596
                     /gene="clpA"
                     /locus_tag="ID725_RS08745"
                     /old_locus_tag="pgaptmp_001749"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGV7850371.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(21942..22029)
                     /locus_tag="ID725_RS08750"
                     /old_locus_tag="pgaptmp_001750"
     tRNA            complement(21942..22029)
                     /locus_tag="ID725_RS08750"
                     /old_locus_tag="pgaptmp_001750"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21993..21995),aa:Ser,seq:gga)
     gene            complement(22283..22501)
                     /gene="infA"
                     /locus_tag="ID725_RS08755"
                     /old_locus_tag="pgaptmp_001751"
     CDS             complement(22283..22501)
                     /gene="infA"
                     /locus_tag="ID725_RS08755"
                     /old_locus_tag="pgaptmp_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGV7850372.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(22786..23490)
                     /gene="aat"
                     /locus_tag="ID725_RS08760"
                     /old_locus_tag="pgaptmp_001752"
     CDS             complement(22786..23490)
                     /gene="aat"
                     /locus_tag="ID725_RS08760"
                     /old_locus_tag="pgaptmp_001752"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGV7850373.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(23532..25253)
                     /gene="cydC"
                     /locus_tag="ID725_RS08765"
                     /old_locus_tag="pgaptmp_001753"
     CDS             complement(23532..25253)
                     /gene="cydC"
                     /locus_tag="ID725_RS08765"
                     /old_locus_tag="pgaptmp_001753"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGV7850374.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(25254..27020)
                     /gene="cydD"
                     /locus_tag="ID725_RS08770"
                     /old_locus_tag="pgaptmp_001754"
     CDS             complement(25254..27020)
                     /gene="cydD"
                     /locus_tag="ID725_RS08770"
                     /old_locus_tag="pgaptmp_001754"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGV7850375.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(27143..28108)
                     /gene="trxB"
                     /locus_tag="ID725_RS08775"
                     /old_locus_tag="pgaptmp_001755"
     CDS             complement(27143..28108)
                     /gene="trxB"
                     /locus_tag="ID725_RS08775"
                     /old_locus_tag="pgaptmp_001755"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGV7850376.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            28653..29147
                     /gene="lrp"
                     /locus_tag="ID725_RS08780"
                     /old_locus_tag="pgaptmp_001756"
     CDS             28653..29147
                     /gene="lrp"
                     /locus_tag="ID725_RS08780"
                     /old_locus_tag="pgaptmp_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGV7850377.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            29282..33310
                     /gene="ftsK"
                     /locus_tag="ID725_RS08785"
                     /old_locus_tag="pgaptmp_001757"
     CDS             29282..33310
                     /gene="ftsK"
                     /locus_tag="ID725_RS08785"
                     /old_locus_tag="pgaptmp_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGV7850378.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            33465..34076
                     /gene="lolA"
                     /locus_tag="ID725_RS08790"
                     /old_locus_tag="pgaptmp_001758"
     CDS             33465..34076
                     /gene="lolA"
                     /locus_tag="ID725_RS08790"
                     /old_locus_tag="pgaptmp_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGV7850379.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            34087..35430
                     /gene="rarA"
                     /locus_tag="ID725_RS08795"
                     /old_locus_tag="pgaptmp_001759"
                     /gene_synonym="mgsA"
     CDS             34087..35430
                     /gene="rarA"
                     /locus_tag="ID725_RS08795"
                     /old_locus_tag="pgaptmp_001759"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGV7850380.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            35521..36813
                     /gene="serS"
                     /locus_tag="ID725_RS08800"
                     /old_locus_tag="pgaptmp_001760"
     CDS             35521..36813
                     /gene="serS"
                     /locus_tag="ID725_RS08800"
                     /old_locus_tag="pgaptmp_001760"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGV7850381.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            37052..39496
                     /gene="dmsA"
                     /locus_tag="ID725_RS08805"
                     /old_locus_tag="pgaptmp_001761"
     CDS             37052..39496
                     /gene="dmsA"
                     /locus_tag="ID725_RS08805"
                     /old_locus_tag="pgaptmp_001761"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGV7850382.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            39507..40124
                     /gene="dmsB"
                     /locus_tag="ID725_RS08810"
                     /old_locus_tag="pgaptmp_001762"
     CDS             39507..40124
                     /gene="dmsB"
                     /locus_tag="ID725_RS08810"
                     /old_locus_tag="pgaptmp_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGV7850383.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            40126..40989
                     /gene="dmsC"
                     /locus_tag="ID725_RS08815"
                     /old_locus_tag="pgaptmp_001763"
     CDS             40126..40989
                     /gene="dmsC"
                     /locus_tag="ID725_RS08815"
                     /old_locus_tag="pgaptmp_001763"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGV7850384.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(41025..41651)
                     /gene="ycaC"
                     /locus_tag="ID725_RS08820"
                     /old_locus_tag="pgaptmp_001764"
     CDS             complement(41025..41651)
                     /gene="ycaC"
                     /locus_tag="ID725_RS08820"
                     /old_locus_tag="pgaptmp_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGV7850385.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            41966..43114
                     /gene="ycaD"
                     /locus_tag="ID725_RS08825"
                     /old_locus_tag="pgaptmp_001765"
     CDS             41966..43114
                     /gene="ycaD"
                     /locus_tag="ID725_RS08825"
                     /old_locus_tag="pgaptmp_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850386.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            43324..44754
                     /gene="ycaM"
                     /locus_tag="ID725_RS08830"
                     /old_locus_tag="pgaptmp_001766"
     CDS             43324..44754
                     /gene="ycaM"
                     /locus_tag="ID725_RS08830"
                     /old_locus_tag="pgaptmp_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGV7850387.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(44755..44865)
                     /locus_tag="ID725_RS08835"
                     /old_locus_tag="pgaptmp_001767"
                     /pseudo
     CDS             complement(44755..44865)
                     /locus_tag="ID725_RS08835"
                     /old_locus_tag="pgaptmp_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(44964..45704)
                     /gene="pflA"
                     /locus_tag="ID725_RS08840"
                     /old_locus_tag="pgaptmp_001768"
     CDS             complement(44964..45704)
                     /gene="pflA"
                     /locus_tag="ID725_RS08840"
                     /old_locus_tag="pgaptmp_001768"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGV7850388.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(45896..48178)
                     /gene="pflB"
                     /locus_tag="ID725_RS08845"
                     /old_locus_tag="pgaptmp_001769"
     CDS             complement(45896..48178)
                     /gene="pflB"
                     /locus_tag="ID725_RS08845"
                     /old_locus_tag="pgaptmp_001769"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGV7850389.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(48233..49090)
                     /gene="focA"
                     /locus_tag="ID725_RS08850"
                     /old_locus_tag="pgaptmp_001770"
     CDS             complement(48233..49090)
                     /gene="focA"
                     /locus_tag="ID725_RS08850"
                     /old_locus_tag="pgaptmp_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGV7850390.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        12094 a        12270 c        13057 g        11764 t
ORIGIN      
        1 tcattttttt aatgttgata tgataagagt taatttcagc aaatgtcttt ttcatgactg
       61 tagcatgcat ggtgtcaaga taaaaccatg gttgccagta aaatggacaa aagagttaat
      121 aaatgattat ttgtatggct gtttactctc tctgtactct atctgtgcaa gagatatata
      181 taatatgaac gccgggaata acgtgaaggt tgctgcggat gcttttcttg aaatacttta
      241 ttctcttaaa aataaatatt gtattaaact attaagcgct caggatcggg cgtttatata
      301 cgaatttgca agaatgatct tcgcttatat taatgacaaa agcattgaga tcttattact
      361 ttcttgtttt gcggcggcgg accaaaaagc aatccagcgg tatatacctc aggcgcaaga
      421 tggggaagac tttaggaatc atttacaata taagttgact cttcccactc attaatgttg
      481 ccggcacaac ataatagggc aggtaagcgg tgctccataa tgaaaccaat atccggttac
      541 gaaacagata attgtacagg cttatgtagc tgtttttgac acaatgagat gatttcctaa
      601 actgatcaaa accttcttcc caacatggca tatcagtccg ataatgcttt agagatcgaa
      661 ggcttaacaa acaaggagat cgatcatgca attttccaca actccaactc tggaaggcca
      721 gaccatcgtt gaatattgcg gtgtggtgac tggcgaagcg attttaggcg cgaatatttt
      781 ccgtgatttc tttgctggta ttcgcgatat cgttggcgga cgctccggtg cctacgaaaa
      841 agagctacgt aaagcgcggg agatcgcctt tgaggagctt ggctcccagg cgcgggcgct
      901 gggggccgat gccgtagtcg gtattgatat cgactacgaa acggtcgggc aaaacggcag
      961 tatgctgatg gttagcgtca gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc
     1021 tggctgatcg ctgtcgctct gttactggca gggtgcgcag gcgaaaaagg cattgtcgag
     1081 aaagagggat atcagcttga tacccgacgc caggcgcagg cggcgtatcc gcgcattaaa
     1141 gtgctggtga tccactacac cgcagatgat tttgatagct cgctggcgac actgaccgat
     1201 aagcaggtca gctcgcatta tctggtccct gcggtaccac cgcgatacaa cggtaaaccg
     1261 cgcatctggc aattggtgcc ggaacaagaa ctggcctggc atgcggggat tagcgcctgg
     1321 cgcggggcaa cgcgccttaa cgacacctct attggcattg agctggaaaa ccgtggctgg
     1381 caaaaatcgg cgggggtgaa atattttgcc ccgtttgaac cggcacagat tcaggcgctt
     1441 attccgctgg cgaaagatat tattgcccgt tatcacatca agccggaaaa cgtagtggca
     1501 catgcggata tcgccccgca gcgcaaagac gatccggggc cattatttcc ctggcagcaa
     1561 ctggcgcagc aggggattgg tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc
     1621 gggcgcgcgc cgcacacacc ggtagatacc gcgtcattgc tggagctttt ggcgcgctac
     1681 ggttatgacg ttaaacctga tatgacaccg cgcgagcagc ggcgcgtgat tatggcgttc
     1741 cagatgcatt tccgcccgac gttatataac ggcgaagcgg atgcagaaac tcaggcgatt
     1801 gccgaagcgt tgctggagaa atacgggcag gattagcgcg gcagttttcc gtggtcgcgc
     1861 agccaggcgg cagttttctc gataccttca tccagggtga tgaccggctg ataacctaac
     1921 tcttcctgtg cccgcgtaat atccagcgta aagtcaaaat taagcttcga gacgccgtag
     1981 tgggtcagcg gcggctcttt tgctgacttg cggcctaaac gctccatgct gcgggcgatc
     2041 atatccagca tcgggtaagg gacggaacga atacgacagt caatattcaa ctcgtcgatc
     2101 agcttctgca cgatgctgcg cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc
     2161 ccggaaggta gcttatcgca ggcttcctgg cttgccagcc acatggcgtg cacggcattt
     2221 tcatagtagg tcatatccac cagcgcactg ccgccatgcg gtaacagaat gctgccgtag
     2281 tggtgcatca tatgcgcaag acgggggata aagactttat cgtgcggtcc gaacagactt
     2341 tgtgggcgca gaatagtaaa gcgcgtttgt ggattcgcct gcgaaagcat attgatcact
     2401 tcttcgctgg ccgctttgct gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct
     2461 tctttaatat cgcgatggtg gtgataatcg aagtacaggg agggagaaga gatatgaata
     2521 aagttacgta caccccaggc gacagcccat tcacctaggc ggcgagtggc gcgaacgtta
     2581 gccagatcga aagcctgttg tgtcccccag ggagaggtaa agctggagca gtgccacagc
     2641 gtatcaatgc ccgcgagcat cactttagct tgtgatgaga ccagctcggt cagatccgcc
     2701 ggaacaaact ctgcgcccat tttttccagc aatttaccca ttgcctcgtt gcgaccggtc
     2761 gctcgcacgc tgatgccttt ctggcataaa aactctaccg cgtttcgacc taagccgctg
     2821 gtggcgccgg taaccagtac cttcatatca atccactgtt gttgagaaaa tgacgtgcgc
     2881 attcttccgt gatttccccc ttgatgcaat gggaaacatg aaagaataac gcaggttttg
     2941 tcgattaatc tgtgctttgt tctgccagtc tggcgattcg ttttgccatt ccgcgaaaaa
     3001 taaacagatg cgcggggatc atcaataacc agtaaaacag ccccggcata ccgtgcggat
     3061 gccagaaagc gcggacatcg atagtacgat agtcgccttt atcttccagg gtaaaacaca
     3121 gtcgtcccag ccccggtgct ttcatgccaa ataacaacgt aagttgtttt tccggttcaa
     3181 cgacaatcac tttccagcta tccaccgcat cgccagtttg caaatattcg cgctccggac
     3241 ggcctttcgc taatttatga ccgatcgcac ggtccatcaa cgcccgtgtc tgccacaaaa
     3301 tattgccaaa gaaataacgc tctttaccgc cgatttggtt caccacctgc cataaagccg
     3361 caaggctggc ggacgtttta acggtaaatc ccgcctgttt ggcaaaataa ccatactctg
     3421 gtcgccagcg ggcaaaggcc tgagtgtcgt atccccagtc gctggagttg accagctttt
     3481 cttcttcttt cagggtgcga cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga
     3541 gcgcacgtag cgcggtgtca tccgccagca gatcgtgttt cagcccctga atcaacgccc
     3601 tggcggtggt gggcggtacg gaagtaatca cattgagaaa ccacaccgaa atccagcggg
     3661 tggggagggg gatggggatc aaccagcggc gcttaccgct caccgccata aaatgttcaa
     3721 actgttgctg ataactgagc acctctggtc cggcggcttc gaagatgcgg tgttcgttgg
     3781 ctggatggtc taacagcgcc accagatagt gcagcaagtt ttccagcgcg atgggcgtgg
     3841 tgcgtgaacg tacccagcgt ggcggcgtta acactggcag gttgtagacc atatcgcgca
     3901 tgacttcgaa cgctgctgaa cctgcaccaa cgattattcc ggcacgcaat tcggtcactg
     3961 gtacattcgc ttcacgaaga atgtccgccg tagcctgacg cgcgcgcaga tggtccgact
     4021 gctcatgtgg cggggcctgc aacgaactga gaaagattaa ttgcttaact ggtacttcac
     4081 gtagcgcatc gcggaagttg agagccacct ggcgctcctg agcgataaaa tcgccgcctt
     4141 cgcccatgct atgcaccaga aaatagaccg tatcgatatc ctgtaacagg gccggaaggt
     4201 tatccggcca gctgagatcg actttatggc aactgacgtt tgccagttgc agctttgcaa
     4261 gcctgtcgac atgacgtgcc gccgccagga tctgatgccc ttgctggctg agtgtgcgca
     4321 ccagatgctg accaatgtag ccactggcac caagaactaa aatgcgttgc ggcacgtctc
     4381 cctccttaac gcgccaggaa tgcgcgccag tgggcggcga cttctgccag ttgttcgcgc
     4441 gagacgtcaa gatgggtcac caggcggaca atcggcgagg cgttaatcag tacgtttctc
     4501 gctttcatgt attcgcctaa cgcggcagca ttttcttcgc cgacgcgaac aaacagcata
     4561 ttagtgtcct gacgcatcac atccgcgcct gcttcacgca gttgctccgc catccaggcg
     4621 gcgttgtcgt gatcttcctg caaccgtgcg acgttatttt tcagcgcata catcccggcg
     4681 gcagccagaa tgccggactg gcgcatcccg ccacctgtca ttttccgcca gcgaatggca
     4741 cgtttaatgt aatcacgatt accgacgagt aatgaaccga ctggcgtccc aagacctttc
     4801 gacaggcaaa tggtgaacga atcacaatat tgcgtgagct ctttcagttc gctgccgtaa
     4861 gccaccacgg cattaaagat gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg
     4921 gtaaattccc atgcatcttt aaggtattca cgcggcaaca ctttgccgtt gtgggtgttt
     4981 tccagactga gtaatttggt gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc
     5041 actttatcca gcggtagcgt gccgtcggca gccgcgtcga tgggttgcgg ttgaatactg
     5101 cccagcaccg ccgcgccacc ggcttcaaac agatagttat gcgcggcctg accgacaata
     5161 tactcttcgc cgcgttcgca gtgactgagc agagcgacca ggttggcctg agtgccggta
     5221 ggcagaaaaa tggcggcttc tttaccggaa agctctgctg catagtcctg cagagcatta
     5281 acggtagggt cgtctccgta aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc
     5341 atggcgcggc tcggtcgggt tacggtatca ctgcgtaaat caatcatggc atgtccttat
     5401 tatgacggga aatgccaccc tttttacctt agccagttcg ttttcgccag ttcgatcact
     5461 tcatcaccgc gtccgctgat gattgcgcgc agcatataca ggctgaaacc tttggcctgt
     5521 tcgagtttga tctgcggtgg aatggctaac tcttctttgg cgaccaccac atccaccaat
     5581 accggaccgt cgatggagaa ggcgcgttgc agggcttcat cgatttcaga ggctttttct
     5641 acacgaatac ccgtaattcc gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc
     5701 tcagtaccgt ctgtcaggta gcctccggct ttcatctcca tcgccacaaa gcccagcacg
     5761 ctgttattaa agacgataat tttcaccggc agtttcatct gcactaccga gaggaaatcg
     5821 cccatcaaca tgctaaaacc gccatcgccg cacatggcga ccacctgacg ttccggctcg
     5881 gttgcctgcg cgcccagcgc ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa
     5941 cctaacaggc gacgcttgcc gttcattttc agataacgcg ccgcccacac cgttggcgtg
     6001 ccgacgtcac aggtaaagat ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga
     6061 tattgcgggt gaatggcttt ctcgctcggt ttagctaaat cgtccagccc tttgcgggca
     6121 tcgcggtaat cttccagcgc tttatccaga aacttgcgat cggctttttc ttccaccaat
     6181 ggcagcaatg cacgcagggt cgacttgata tcgccgacca gcgccatatc caccttgctg
     6241 tgtgcgccga tgctggctgg gttgatatca atctgaataa ttttggcatc ggtcgggtag
     6301 aacgcacggt aggggaactg agtaccgagt agcactaacg tgtcggcgtt catcatggta
     6361 tggaaacctg acgagaatcc gattaacccg gtcattccaa catcatacgg attatcgtat
     6421 tcgacatgct ctttaccgcg tagggcatga acaataggtg ctttaatttt cccggcaaac
     6481 tcaactaact ctttatgcgc ccccgcgcag ccgctgccac acatcagagc gatattgctg
     6541 gaataacgca gcagttgcgc cagtttgcgt aactcttctt cttccggcgt cacgactggc
     6601 tgtggcgcat gataccagtg ggtagttgcc ccttctggcg caggttttaa cgccacgtcg
     6661 cccggtaaca cgacaacgga aacgccacgg ttaagcaccg ctttgcgcat ggcaatcgcc
     6721 agcacttgtg ggatctgctc cgggctggaa accagctcgc aatagtgact acattcgcgg
     6781 aatagctctt gtgggtgggt ttcctggaaa tagccgctgc caatttcgct ggaaggaata
     6841 tgagcggcaa tcgccagtac cggaacgtga ttgcggtggc aatcgaacag gccgttgatt
     6901 aagtgcaggt tgccggggcc gcacgatcca gcacagaccg ccagttctcc tgaaagttgt
     6961 gcttcagcgc cagcggcaaa cgccgccact tcttcatggc gggtggacat ccactcgatg
     7021 gtgcccatgc gattaagact gtcgctaaga ccgttcagag agtcgcccgt gactccccag
     7081 atgcgtttca cgcctgccga ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc
     7141 atggttctcc atctcctgaa tgtgataacg gtaacaagtt tagttcatct gacggagggg
     7201 gaagggatgg gagagaaagg aggcactaac ggttaaatag cccgatgaaa ggaatatcat
     7261 cgggcataag gcgattatgc gagaaccaaa tccccctgcg gatggcagga gcaggccagt
     7321 acgtaacctt cagcgatttc ggcgtcggtc agcgtcattg tactgctcac cgtatattca
     7381 ccggaaacca ctttcgtctt acagcagccg caaacacccg cgcggcaggc ggcgacaacc
     7441 ggaacgttat tgctttccag cgcctccagc agcgtggtgc caacaggagc gtaaaattct
     7501 tgcgccggtt gcagtttggt gaatttcaga ccgctggtcg cagcttccgc tactggggtg
     7561 aagaatttct ctttaaagaa acgcgtcacg ccgagtgctt tcacttcctg ctctacccaa
     7621 tccatatacg gagccgggcc gcaggtcatc acggtacgtg aagctaagtc aggaacgcga
     7681 gtcaggagtt cgcgagtgag acgaccagcg ataaagcctt cggtaacgtt attttccgcc
     7741 accagcgtta ccggatagtt acgccactca tcggcgaaaa taacatcctg cggcgtacgc
     7801 acgttgtaga tcacccgcac atcggcctgt ggacggttct tcgccagcca gcgacgcatc
     7861 gacataatcg gcgtgacgcc gcagcctgca gccagcaaca ggaatttatc ttctgcttta
     7921 tcgtcgcagg taaattcccc catcgcgtct gaaagccaga gataatcacc gcgttttaca
     7981 tcgcgggtca gccactggga gccgacaccg tcatcaatcc gccgcacggt cagggtgata
     8041 tattcactca cgcctggcgt ggaggaaatg gtgtaagcac gcagcgtttc cgctgagtta
     8101 cgcacgctga ccagtgcata ttgcccggcg cggtatgggt agtaatcgtg gcaaatcagg
     8161 gaaatcgtcc acacatccgg cgtttcttgc ttaatgtgat gaacctgcat ccgccacggg
     8221 cattgattcg ttggcatcgt catcgacaaa ctccttacgc gctcaacagt tgcttcatgt
     8281 cttcttcaac agtggtgata gaacgcaggc cgaatttctc gttcagcacc gccagcagat
     8341 ctggtgtcag gaaaccaggt gcagtcgggc cggtgacgat atttttcacg cccagagaaa
     8401 gcagcgtcag cagaatgaca atcgctttct gttcaaacca ggagagcacc agcgacagcg
     8461 gcagatcgtt gacaccgcag cccagttttt ctgccagcgt aaccgccaga ataattgctg
     8521 agtaagcatc gttacactga ccggcatcca ccagacgagg cagaccttcg atatcgccaa
     8581 actcaagttt gttaaagcga tatttaccac aggcgagagt caggatcagg cagtcatctg
     8641 gcacgctggt ggcgaaatcg gtgaagtagt gacgttcgcc gcgtgcgccg tcacagccac
     8701 caagcaggaa gatatgacgc agtttttcac ggctcaccag atcaattagc gtatccgctg
     8761 cgccaagcag cgtctggcgg ccaaagccca cggtgatcag gtgcggaatt tcgctgtatg
     8821 ggaagcctgc catttgttgc gcctgggcaa tgaccgcaga gaaatcttcg ccatccagat
     8881 gacgcacgcc aggccagcca acgatgctgc gggtccagat acgatcgtca taagcgccta
     8941 cggttgggtc gatgatgcag ttcgaggtca tcacgatggg gccagggaaa cgagcgaact
     9001 ccacttgctg attctgccag ccgctgccgt agttaccgac cagatgcttg aatttacgca
     9061 gctccgggta gccatgcgca ggcagcattt cgccgtgggt gtagacatta acgcccgtgc
     9121 cttcggtctg ttccagcagg ttgtagagat ctttgagatc gtgaccggaa atcaggatgc
     9181 atttacccgc cgtcgctttg acgttgacct gggttggcgt cgggtgaccg tatttaccgg
     9241 tttcgcctgc atccagaatg ctcatcactt tgaagttcat ctggccgatt tccattgaac
     9301 actcaagaag cgcgttcata tcggcaggcc aggtccccag ccacgccatg attttatgat
     9361 actgggcata aatatcgttg tcgtattgac cgagaacgtg tgcgtgttcc atataggccg
     9421 ccgcaccttt caggccatac aggcacagca gacgcaggcc gagaatgttt tcgccaatcg
     9481 ccgctttatc tttgttaggg gtaaattctg ctgcctgacg ttgcagttcg ccgagatcgt
     9541 cgctcaccag ttgcaggtca gccatcgggt tatcgacgcg ggcgttggca tctacagcca
     9601 ggcattgtgc tttcagcgcc tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac
     9661 gcggagaatc gaagttaacg ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt
     9721 cgacatcgtg gttgatgatg ccgtattcac gcgcttttac cgcccaggca gaaagccctt
     9781 gcagcgccgc gatgagtaaa tcctgaaggt cagaagtttc cgccgtttta ccacacatcc
     9841 cctgcgcgta tgagcagccg tttcctgccg gagtacggat agtttgttca cattgcacac
     9901 aaaacatgat cacacctttt aaagttatat ttaatataca tgtttaaggt taagacgctt
     9961 aacgcgggga taaaagggat ttttcatgca actttaaggg agattgattt agcgcaattt
    10021 tggcggcagg gatctaccgc cagagaggta ttacgcggag aaaaaggcga tgaggatcgg
    10081 cactaacagg ctaagaataa aaccgtgaac aattgccgcc gggaccatat ccagcccgcc
    10141 agtacgttga agaacgggca gggtgaaatc catcgacgtc gcaccgcata agcccagtgc
    10201 ggtagagcgg ctgcggcgaa tcagcccagg gatcaacata attgcaatca gctcacgggc
    10261 cagatcatta aaaaacgccg cgctcccgat taccggacca aaagattcgg tcaataaaat
    10321 accggaaaga gaataccagc cgaaaccaga ggccattgcc agcgcggtat tgatgggtag
    10381 atcaagaata aaggcgttaa ttaaaccacc aattaatgaa ctggcaacca ccaccacggc
    10441 gacaatcatt ccccggcgat taaggacaat ctgctttaag gtcatgccat tattgcgcaa
    10501 ctgaataccg acgaggaaaa gtagcagaat taacgtgtat tcactggctt cggtcgcgtg
    10561 ttgtaagaaa gccagtccac tcaggccaat ggcaaaacca atcactacta cgccgcacag
    10621 ttttagcgac tcaagcgcca tcgcaatacg cgacgggagt ttttcttgct gatggtggtt
    10681 gcgccacggc agaccgcgct ccagccacat aagggcggca atattacaca gtaaaataac
    10741 ggtaatactg acggcagaat aatgcagaat cgccaacagg ttactggcga ggttatcgag
    10801 aaacgccaga ctgataccca taaaaaagag aataaggtaa accatccagc ttaatagctg
    10861 attaataact tttaacgcag cttgttggcg aagcggaatg aggtaaccca caatcagggg
    10921 aaccagaatg attaacaacc cagaaaacat gaaaaaccag cccttgcaaa gaaagaagtc
    10981 gaaatgcgcg atgacacact actgaaagcg gaaggacgag taaagttgca attaaaagga
    11041 aatgttatgc atcagagggg gatttttaat tctatatgag tgtttagcat tgttgcgctc
    11101 ctttcatgtg caatgccata taaagccact ggcgcaggga gcgcgcaggg ggcggccagt
    11161 cgccgccgcc ccctgcaccc ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt
    11221 cagcttatcc ccttcggcaa ccgggtcggg cgcgaggtaa cgtcctgtaa gacgcgccct
    11281 cagcccacat ccatgtgggc tgccccggcc ttcggggaac gcttcagcga ttttgacgcc
    11341 accaataccc gtgcggtttg taatgtcgaa gataaaacaa ccagggcaga taaaatgcca
    11401 atgctaatta attacgcttt tccagcagag tccggtaaat cagaccaccg ataatgccac
    11461 cgacaattgg caccacccag aagaaccaca gttgttctaa tgcccagccg ccctggaaga
    11521 tggcaaccgc ggtgctgcgc gccgggttaa cagaagtgtt agtcaccgga atactgatta
    11581 agtgaatcag ggttaaggcc agaccaatag cgatcggcgc aaaacctgcc ggagcgaatt
    11641 tgtcggttgc accgtggatc actaacagga agcccgcact caataccagt tcaactacca
    11701 gcgcggaaag catggaataa ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac
    11761 cgctggctgc cgcgtcaaaa cccgttttac cactggcaat taaatacagc agcgctgccg
    11821 caacaatacc gccgacaacc tgggcaatta cgtagccaac gacttctttt gccggaaaac
    11881 gtccgccagc ccataaacca atagtgaccg ccgggttaaa atgaccacca gaaatatgac
    11941 caacagcaaa ggccatcgtc agaacggtca gaccgaacgc caacgccacg ccggcaaaac
    12001 caatgcctaa ttccgggaag cctgcggcca gtacagcact accacagcca ccaaaaacaa
    12061 gccagaaagt accaaaacat tcagctgcta attttctgaa catatccacc tcaattaaaa
    12121 attgaccctg tgaaaaatat ggtcgtttta tagggtcgtc gtaaaaaagt gacgacggaa
    12181 ataatgcgcg gctattttaa aaacgaaggc gagtcattca ccagataaat aaatccagta
    12241 aatttgattt agggcaacag cgggttgccc catatagtca tttgtctgat tgacagtgta
    12301 gtgcaagcaa aagatttaat cctttaggcg taataaaaaa taatttatca tgctaattat
    12361 ttgattttgt tgtttttgca gacttatcag caagagggag tataacgcga ttattcgctc
    12421 atttttcaga catttgccat gcttaaatgt gatgtcatca cgtattagca aggcctttcc
    12481 cgttatactg ccagcgtaaa ggataagtca catatttctg gaggggatat gattcttgag
    12541 cgcgttgaaa ttgtgggttt tcgcggtatc aaccgtttgt cgttgatgct ggaacaaaac
    12601 aacgtcctga ttggggagaa cgcgtggggt aaatccagct tgctggacgc cttaaccctg
    12661 ctgctatcgc cagaatcaga tctctaccat tttgagcgcg acgatttctg gttcccgccg
    12721 ggagatatca acgggcgaga acatcatctg catattattt tgaccttccg cgaatcgctg
    12781 ccaggtcgac atcgggttcg ccgttatcgg ccgctggaag cgtgctggac gccatgcacc
    12841 gatggctatc accgtatttt ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg
    12901 atgacactgc gcagttttct cgataaagac ggacatccga ttgatgttga ggatattaac
    12961 gatcaggcac accatctggt gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt
    13021 atgcgccgta ttcgtaacgg cacggtgcca aatgtcccca atgtggaagt caccgcgcgc
    13081 cagctcgatt tcctcgcccg tgagttgtcc tcacatccgc aaaatctctc tgatggacag
    13141 attcgtcagg gactttccgc aatggtgcag ttgcttgagc attatttctc tgagcagggg
    13201 gctggacagg cgcgatatcg tttaatgcgg cggcgggcca gcaatgagca acgaagctgg
    13261 cgctatctgg acatcatcaa ccggatgatt gaccgacctg gagggcgctc gtatcgggtt
    13321 attttgctcg gcctgtttgc tactttattg caggcaaaag gcacattgcg actggataaa
    13381 gacgcccgtc cattgttgct gatcgaagat ccagaaacgc gtttacaccc cattatgctt
    13441 tcagttgcct ggcatctgtt gaatcttttg cctttacaac gtattgctac caccaactct
    13501 ggcgagctgc tttcgttaac tccagtggaa catgtttgcc gcctggtgcg tgaatcttct
    13561 cgtgttgccg cctggcgtct ggggccaagt ggcttgagca ccgaagacag ccgccgcatc
    13621 tcttttcaca ttcgttttaa tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg
    13681 gaaggggaaa cggaaacctg ggttatcaat gaactggcgc gtcagtgcgg acatcatttt
    13741 gatgccgaag ggatcaaggt cattgagttt gcccagtccg ggctaaagcc actggttaaa
    13801 tttgcccgcc gaatggggat tgaatggcat gtactggtcg atggcgatga agcagggaag
    13861 aaatatgccg ctacggtacg cagcctgttg aataatgatc gggaagccga acgagaacat
    13921 ttaacggcgt taccggcgct ggatatggaa cattttatgt atcgccaggg attttccgat
    13981 gtgttccacc gcgtggcgca aatcccggaa aatgtcccga tgaatctgcg caaaattatc
    14041 tcgaaagcga tccatcgctc ttccaaaccc gatcttgcca ttgaagtggc aatggaggcc
    14101 ggacgtcgtg gtgtggactc cgtaccgacg ctgctgaaaa aaatgttctc acgcgtgctg
    14161 tggctggcgc gcggtcacgc ggattaaccg cgaaacatcg tggccatttg tggctgaata
    14221 gcgtcgagca tctcatagcg ccgacggtat tcagcccgtt ttttactggc gatttcggca
    14281 atctcttttc gtgctatctg tgctggaagg cggtaatggc gttcagcatc acatacgccg
    14341 ccaaccgatt cccagaaagc gttgtaatcg gcatggatct tgccttcttt atcgcgataa
    14401 cgcaggctgc ggtaaatatg cgtttcattg ctgacggcaa taatctgctc tacctgcaaa
    14461 cgttgggcaa acagacaggc cgcttccatc acgaggcgtt tgggaaatag cccgtggcag
    14521 gctttcgtcg cattctggat ttcctgatgt ggaatttccc attttgcgcc ttgcagtcca
    14581 ccaataaaca tcgttctttt cccctgatat tcacacaggg taaacgtgat ttctgccaga
    14641 ggaatacctt cgctgttgcg gaacaggatt gtgctgtcac cttctttatc cattgagatc
    14701 atcatggtca gctcaagcgt gaactgctcg ccgtttttgc cttccagctt cgccagttgc
    14761 agcccggggg tattcaaata taagctgaat tcttccgccg acatacatcc acggagtaac
    14821 gcataatggt aacgtaacgc ctccagcaat tgcttacggc taagattcgc cgcaaggtaa
    14881 gggcgatgca gacgcacagg cagtcgcggc tggcgcgtta acaatacatt gagattaggc
    14941 cagtgggaaa gttcgttcat ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc
    15001 aaaaatttct ggcgaaaact acggcgatgc cagaatttac ccggccgaca ctgtccacgt
    15061 gccagactaa gaaaaagtga caggctgctg agagattcag atgacgtaaa ggtccgttcg
    15121 gttagctgcg acatattcat gaaatcaatg gttgtacatg acgtcgattt caccattgcg
    15181 tatcttaacc aaacatcaat agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat
    15241 ttcgttgaag ttaatgaccc ggattggcat atggagtatt cagaatattt atgaaaaagc
    15301 ggaaaaccgt gaagaagcgt tacgttattg cgctggtgat agtcatcgcc ggactgatta
    15361 cgttatggag aattcttaac gcacccgtgc cgacttatca gacactgatt gtgcgccccg
    15421 gtgatttaca gcaaagcgtg ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg
    15481 tgggcgcgca ggtcagcggt cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa
    15541 aaaaagacca gcttttaggg gttattgatc ctgaacaggc tgaaaaccag atcaaggagg
    15601 tcgaagcaac gctgatggag ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac
    15661 tggcgcgggt gacgtattcc cgtcagcaac gtctggcaca aacgcaggcg gtttcactgc
    15721 aggatctcga caccgccgcg acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg
    15781 acgcgcaaat caagcgcaat caggcttctc tcgatacggc taaaaccaat ctcgattaca
    15841 ctcgcatcgt tgccccgatg gccggggaag tcacgcaaat caccactctg caaggccaga
    15901 cggtgattgc cgcacaacaa gcaccgaaca ttctgacgct ggcagatatg agcaccatgc
    15961 tggtaaaagc gcaggtttct gaagcggatg taatccacct gaagccgggg caaaaagcct
    16021 ggtttacggt acttggcgat ccactgacgc gctacgaggg gcaaatcaag gatgtactac
    16081 cgacgccgga aaaggttaac gacgctattt tctattacgc ccgttttgaa gtccccaacc
    16141 ccaatggttt gctgcggctg gatatgactg cgcaagtgca tattcagctc accgatgtga
    16201 aaaatgtgct gacgatccct ctgtcggcgt taggcgatcc ggttggcgat aatcgttata
    16261 aagtcaaatt gttgcgtaat ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta
    16321 acgataccga tgttgagatt gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg
    16381 aggccaaacc aggagctgca caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag
    16441 ctatcctgcc ggtgatgagc aggttgaggt gctgaagggc atcaccctcg atatttatgc
    16501 cggtgagatg gtagcgattg ttggcgcttc gggttccggt aaatcgaccc tgatgaatat
    16561 tctcggctgt ctggataagg ccaccagcgg cacctatcgc gtcgccggtc aggatgttgc
    16621 cacgctggac gccgatgcgc tggcgcaact gcgccgcgag catttcggct ttattttcca
    16681 gcgttaccat ttgctttcgc atttaaccgc cgagcagaac gttgaagtac cagccgtcta
    16741 tgctggtctt gagcggaaac agcggctgct tcgcgcccag gagttgctgc aacgactggg
    16801 gctggaagac cgtacagagt attatccggc acagctttcg ggtggtcagc aacagcgcgt
    16861 tagcatcgcg cgggcattga tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg
    16921 cgcactggac agccattcag gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg
    16981 cgggcatacg gtgattatcg tcactcacga tccgcaggtc gctgctcagg ccgagcgggt
    17041 gatcgaaatt cgcgacggcg aaattgtgcg caatcctccc gccattgaaa aagtgaatgt
    17101 tgctggcggg acggaacctg ttgtcaacac ggcgtctggc tggcggcagt ttgtcagcgg
    17161 ttttaatgag gcgctgacga tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt
    17221 actgaccatg ctggggatta taatcggtat tgcgtcggtg gtttccattg tcgtggtggg
    17281 cgacgccgcc aaacaaatgg tgctggcgga tattcgttct attggtacga atactattga
    17341 tgtctatccc gggaaagatt ttggcgatga cgatccgcaa tatcaacagg cgctgaagta
    17401 cgatgactta atcgccatcc aaaaacaacc gtgggtcgct tcagccacac ctgccgtctc
    17461 gcaaaacctg cgcctgcgtt ataacaatgt tgatgttgct gccagtgcca atggcgtgag
    17521 cggcgattat tttaatgtct atggcatgac cttcagtgaa ggaaacacct ttaaccagga
    17581 acagctgaac ggtcgtgcgc aggtcgtggt tctcgacagt aatactcgcc gccagctttt
    17641 cccccataaa gcagatgtgg ttggcgaggt gattctggtc ggcaatatgc ccgccagagt
    17701 cattggtgtg gcggaagaaa aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg
    17761 gctaccttac agcacgatgt ccgggcgagt tatgggccag tcgtggctga actccattac
    17821 tgtcagggtg aaagaaggat ttgacagcgc cgaggcggaa cagcaactca cgcgtttact
    17881 ttcactgcgc cacggtaaga aggatttctt tacctggaac atggacggcg tcttgaaaac
    17941 cgttgaaaag accacacgta ctttacaact gtttatgacg ctggtggcgg tgatttcgct
    18001 ggtagtgggc ggtattggtg taatgaatat tatgctggtg tcagtgaccg agcggacgcg
    18061 ggaaattggc attcgcatgg ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct
    18121 gatcgaagcc gtactggttt gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt
    18181 aattgctttc accttgcagc ttttcttacc cggctgggag attggttttt caccgttggc
    18241 actgctgctg gcgtttctct gctcgacggt caccgggatt ttatttggct ggttacccgc
    18301 acgaaatgcg gcacgactgg atccagtaga tgctctggca cgagagtaat ttttgagata
    18361 aaaatgccag ccgatcgggc tggcattttg cctttaggat gtacacaatg agacagaaga
    18421 gctatgcgac tgccgcttct acttcgacgg gcacaataac actggcgtga ttgccttttg
    18481 gcccctggtg gacatcaaac tgaacggatt gtccagcttt tagcgttctg taaccatcca
    18541 tctgaatggt ggaataatga gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac
    18601 caaacccttt ggcattgttg aaccacttaa cagtaccctt ttccatgctt cgacatcctt
    18661 cgcaaatctt atacaagtaa gatggaataa accggggtca gagagggggc tgttcaaaac
    18721 ctcgccaact ctagaaatac aatttagaga attagggcga gccgtcaagc atttgacagg
    18781 ggacaggggg caggtatgaa tcaaaaattt gaagcagtta acgctattga caggaatgtg
    18841 acagatgtcg ctgatgccaa cgatagatga tagttatcta tcatgtggag tagattggtc
    18901 aggcaaataa gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat
    18961 aactgacaat gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag
    19021 ttcgcgacgc gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattaca
    19081 ctccgatgga gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg
    19141 caacgcaatt gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg
    19201 ccgaggttgc agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc
    19261 cattgctgtg tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta
    19321 tgctcaatca agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc
    19381 gtcatgagtt tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc
    19441 gggaggcgct ggaagcgtgt tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct
    19501 ttattgaaca aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga
    19561 cgctgagttt tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca
    19621 atgaggttac cggtgcaaac gttctggtcg ctatctttag cgaacaggag tcgcaggcgg
    19681 catatctgtt gcgtaaacat gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg
    19741 gcacgcgtaa agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag
    19801 aacaagctgg tggggaggaa cgtatggaga atttcacgac gaacctgaat cagcttgcgc
    19861 gcgtgggcgg aatcgaccca ctgattggtc gtgagaagga gctggagcgt gctattcagg
    19921 ttctctgccg tcgccgtaaa aacaacccgc tgctagtggg ggaatctggt gtcggtaaaa
    19981 ccgcgattgc agaaggtctt gcctggcgaa ttgttcaggg cgatgtgccg gaagtgatgg
    20041 ctgactgtac gatttactct ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg
    20101 gcgactttga aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca
    20161 tcctgtttat tgatgagatc cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg
    20221 ttgatgcggc taacctaatc aaaccgttgc tctccagcgg taaaattcgc gtaattggtt
    20281 cgacaaccta tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct
    20341 tccagaaaat tgatattact gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc
    20401 tgaaaccgaa gtatgaagca caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg
    20461 cggtagagct ggcggtgaaa tacattaacg atcgtcatct gccggataaa gccattgatg
    20521 ttatcgacga agcgggcgct cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg
    20581 ttaatgtggc ggatattgag tccgtggtgg cccgtattgc acgcattcca gagaagagtg
    20641 tttctcagag tgaccgcgat accctgaaaa acctcggcga tcgcctgaaa atgctggtct
    20701 tcggtcagga taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt
    20761 taggtcacga acataaaccg gtcggttcgt tcctgtttgc cggccctacc ggggtcggga
    20821 aaacagaggt gacggtacag ctttcgaaag ctttgggcat tgagcttctg cgctttgata
    20881 tgtccgagta tatggaacgc cataccgtca gccgtcttat tggtgcgcct ccgggatacg
    20941 ttggttttga tcagggcggt ttgctgactg atgcggtcat caagcatcca catgcggtgc
    21001 tgctgctgga cgaaatcgag aaagcgcacc cggacgtgtt caatattctg ttgcaggtga
    21061 tggacaacgg tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtac
    21121 tggtgatgac cactaacgcc ggggtacggg aaactgagcg caaatccatt ggtcttatcc
    21181 accaggataa cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc
    21241 gtaaccgtct cgacaacatt atctggttcg atcatctgtc aactgacgtg atccatcagg
    21301 tggtggataa attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctctgg
    21361 aagtgagcca ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg
    21421 cacgtccgat ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc
    21481 tgtttggttc gctggtggac ggcggtcagg tcaccgtcgc gctggataaa gagaaaaatg
    21541 agctgactta cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatctg
    21601 attgtcaggt aggttggtga agtccgtaat ctcgaaagag gttacggact ttttatttat
    21661 ggggtggaac ggtgaaaacc ctatttttgg aggtgaaggt aatttgttga taattagtgc
    21721 tgctggaaaa ggtaaggata aaaaagggtg acagcaggaa aatgagatgg ttttgcttta
    21781 ttaacaacgg gctaaacgtg tagtatttga gttcactgcc gtacaggcag cttagaaatt
    21841 cactggtaac atactccacc cgcccaccat gttcactgcc gtacaggcag ataaaatgcg
    21901 aaaaaaaagc ccgtactttc gtacgagctc ttctttaaat atggcggtga gggggggatt
    21961 cgaacccccg atacgttgcc gtatacacac tttccaggcg tgctccttca gccactcgga
    22021 cacctcacca aattgttttg ctgccaaacc tcatgggtgg caacggggcg ctactatagg
    22081 gagttggagt aaaacggtca agaagaattt taatgataat tattgtttgc tcatactgta
    22141 aacaacttgt gcagtatatc tacatcgaga caagttatgg acttatactt ccaaagtact
    22201 tcatgcatat cacaaaataa aaaggccggt taaaccgacc ttttactcgt tctttctctt
    22261 cgcccatcag gcggtaaaac aatcagcgac tacggaagac aatgcggcct ttgctcaggt
    22321 cgtacggggt cagttcaaca gtcactttgt cgcccgtcag gatgcggatg tagtttttgc
    22381 gcattttacc ggagatgtgt gcagtaacca cgtgaccgtt ttctaactct acgcggaaca
    22441 tggtattagg caacgtttca agaacggtac cttgcatttc aatattgtct tctttggcca
    22501 tctaatcctc tggggtatca ctaccgtaat ttgaaccggc aagataatgc cgaagttctg
    22561 taaataagta aagatttgcg cgctaaatcg caacaaacag gttcggcaca ttactccgaa
    22621 aacacacggc taagccgcac caaaagcgca acgtataagg gagcggtgag ataaacgatg
    22681 ggcgttacct gacgcgaaaa attccttatc ggcagcgggg taatgagcgt aaccaactct
    22741 gcgaccgcaa ttataacact ctggggagaa atgtgccgaa aacattcatt cttgtggtga
    22801 aaacaagcat cgtggtaccc agaaattatt cggcaatcgt ccgaggcgca tttgattgag
    22861 ataattaagg taattccggc ggggaatttc gcaggcacca agcgatgctg tgtgatcgtt
    22921 aaggacctgg cagtcgataa gcttaccgcc atgaccgata aattcctcac agaataccag
    22981 aagcgccgtt ttagacgcat tttccatccg gctgaacatg gactcgccac aaaatagcgt
    23041 tccctgggcc acgccgtaca taccgccgac aagctcatct tcacgccaga cttcaatgga
    23101 gtgggcatga ccgagttcgt gaaggcgatg gtaggcttcg accacgccac gcgtgatcca
    23161 ggttccttct tcgcgatcgc tggcacagcc ttcaatgacc tgaccaaaag cgtaattcat
    23221 cgtgacacga tagggcgagc gtttatgaaa tcgcttcata ctacggctga tatgcagtga
    23281 ttctggccat agcaccgcgc ggggatcggg cgaccaccag aggatggggt cgcctggaga
    23341 aaaccacgga aaaataccac gctggtaagc cattaacagg cgcgcagggc taagatcgcc
    23401 cccaagtgcc agcaggccgt taggctcacg taatgcgcct tccggggaag ggaaggctat
    23461 tgaatggcga gaaagctgaa ccaggcgcat gaccgcaaaa ctccacgcaa gtcggatcgt
    23521 tcaataatag cttacaaacc ctgcttgaac tggtaataac gcccctgtct ggcaagcagt
    23581 tctgcgtgag taccttgctc aataatttgc ccgttgtcca tcactattat ttgttggaaa
    23641 cgagagagtc cgcgaagtcg atgggtgacc attaacaccg ttttctcacg catcatttct
    23701 gcaagcaatt caaggatctg gctttcggtt gtggcatcta agccttcggt aggttcatcc
    23761 agcaacacca gtggcgcatc atgtaacagc gcacgggcga tagccagacg gcgcagttca
    23821 ccaccggaga gctggcgtcc gccttcacct aaccaactgt tgagacctgc atcctcgagc
    23881 tgcttctcca gaccaacgcg acgtaagatc accgccagag cctcatcact actgccaggc
    23941 gaggcgagta acagattatc acgcagcgtg gcgctaaaca gatgcactcg ctgaggaaca
    24001 acgctgatgg tctgtcgtag agccgcttca ttcaggctgg ctatggggct atcgttaagc
    24061 aaaatctcgc cctgttgcgg gtcccatgcg cgggtcagca gttgtaacag tgttgatttg
    24121 ccgcatccgg ttcgcccgag aatcgctata tgttccccgg cgtttacctg aagagaaatc
    24181 cctttaagtg cctgttggga ttgctccgga taagtgaact gaacatcccg taatgtcagc
    24241 gaaacgcgat cggcaacacg agtttgggta tcagggaagg tgacctccgg tttttgatcc
    24301 gttaagtcag agatacgtac ggcagaggca atgacttgcc ccagatgctg aaatgcaccc
    24361 gttactggtg ccagtgcttc aaacgcggct aacgcgcaaa agacaaacag ggcaattaac
    24421 gcgccgggtt gagcattgcc gccaacgccg ccagacgcca tccacagcat caggatcacc
    24481 gctaacgcgc caatgagcag cattatcgct tgcgacaatg cggtcagttc agattgacgg
    24541 cgttgcgctt ccagccattg aatttctgta ttctctagtt gcgtgcgata acgatcgctg
    24601 gcaccaaaaa tggtcagctc agcttgccct tgcagccagg ccgtcagttg ttggcgatac
    24661 tgtccgcgaa gatgagtcag attttgcccg gtgctttttc ccgcacgata aaacagcggt
    24721 ggcatcagga aaagcgtcag taacataatg ccgcccagcg taaaggcgag ggtggaatca
    24781 aggaaactta acccgattgt caccaccata atcaccacaa aagcgcccac cagcggcgag
    24841 ataacgcgca ggtaaagatg atcgagcgta tcaacatccg ccaccacgcg gttgagcaat
    24901 tcgccctgac gatagcgcgc cagtccggca ggggagaggg gcaacaattt gctgaaggtg
    24961 taaatgcgca gatgctgcaa cacgcggaaa gtcgcgtcgt gacttaccag acgttcaaaa
    25021 taacgcccgg cagtacgggt gattgctgca ccacgcacgc ccgcagcggg tagcatatag
    25081 ttgaagctgt acagtccggc aacccccgca accgctgagg ccgagaggaa ccagccggaa
    25141 agtgtcaaca gaccgatact ggcgagcagc gtcacaattg ccagcacaat accaagactt
    25201 aacatccatt tatgacgttt atacagtgcc agatagggta gcaaagcgcg catttaaatc
    25261 tcctcctgac gatgggccag taatgtggcg aatgggccac cagccacact taattccgcg
    25321 taacgtcctt gctcaataat ccggccatcc tgcataaccc aaatgacatc ccagtcagca
    25381 agatcttcta actggtgggt gaccattaac gttgtctggc gcagagaggc ggcattcagc
    25441 gcctccatta cgcgctgttc actgtgagca tcaaggctgg cagcgggttc atccaacagt
    25501 aatagcgaac agggatttag taacgcacgg gccaccgcca cgcgctgcgc ctgccccacg
    25561 gaaaggcggg cagcctggtc gccaacaggc gtatcaacgc cttgtggcag gagcggtaga
    25621 aactcgctga cccaggcgtt atccagcgct gcttgtaatt cttgttcgct ggcatcaggt
    25681 cgcgccagta gtacgttatc ccgtaatgtt gctgccggta attgtgggtt ttgaccaacc
    25741 caggagagat gtttacgcca tgattctggt gataaatcgc gtaattctat cccgttgatt
    25801 cgtaatgatc cctgatatga gagaaaacca gaaagcgcgt tcagcagtga gcttttacct
    25861 gaaccgctgc gaccaaccaa cactgcacgt tggcctgctg gcaaagtaaa gttcagcggt
    25921 ccggccagcg ttttaccttc cggcgacgtg ataaacagat cctcggcctc aatggtcagc
    25981 ggatcggttg atgctaattc cgtctcaccg cgctgcggat gggcgagcgg ggtttccata
    26041 aacgttttca gactgtcagc tgcaccaaca gcctgggctt tagcatgata aaacgtaccg
    26101 agatcgcgta atggctggaa aaactctggc gcaaggatca gggccagaaa acccgcagcc
    26161 agcgtcacgc cagtatcgta gtgaccaaaa tccagctcgc cgagatagga aaaaccaaag
    26221 tagaccgcca ccagagcaat tgacagcgag gtaaaaaatt cgagaatgcc ggaggataaa
    26281 aacgccagcc gtagcacttc cattgtccgt tggcggaaat cttccgaagc agaacgaata
    26341 ctttcaattt cagcttcacc acgaccaaaa atacgcaatg tttccatgcc gcgcaggcga
    26401 tcgaggaaat gcccacttaa gcgagcaaga gcgagaaagt tacgtcggtt agcatcggca
    26461 gcccccatcc caaccagcgc cataaacaac ggaattaacg gtgcagtgcc cagcagaatg
    26521 agcgccgcag cccagttaga ggggaagatc gccaccacaa tcagcaacgg caccgacact
    26581 gccagcgcca tttgcggcag gtagcgtgca tagtaatcat gcatatcgtc aatttgctcg
    26641 agtaccagcg tcgcccagct ccccgcaggt ttaccctgaa tccacgctgg ccctgcttgt
    26701 tgcagacggt cgagaacctg acggcggatg gcaaagcgga tatgctgccc ggcgtgataa
    26761 cccacccgtt cgcgtaacca gaccacccat gcgcgcagta caaaggtcag aaccagtaac
    26821 gtaaagggaa gcagcagggc ttcacgggga atattctcca taatcatatg ttgcagaatt
    26881 cgcgccatga accatgcctg ggcaatgatc aatatgccgc tcacaaagcc cagcagacga
    26941 gaaatattca gccaacgttg ggagatgacg ctttgctgtt ttaaccagcg ggttaactct
    27001 ttttgacgag atttattcat tgcacgctta gcaggtgagt tatcagaatt atttgcagag
    27061 caatgttaca acggggaaaa aataaaggcg acccatagtc gcatggtgtc gccttcttta
    27121 cttttgttac tgatttgtaa aattattttg cgtcagctaa accatcgagg tagcgttccg
    27181 catcaagtgc tgccatgcag cctgtaccag cagatgtaat ggcctggcga taaatgtgat
    27241 ccatcacgtc gcctgcggca aagacgccag gaatgctggt ctgggtggca ttaccatgaa
    27301 tacccgactg cactttgatg tagccgtttt ccagttccag ctgcccttcg aaaatggcag
    27361 tattcgggct gtgaccgata gcaacaaaca gaccggcaac gtcgagtgac tcgatgttat
    27421 cgctgttttg cgtatcgcgc agacgaacgc cagtgacacc catttgatcg ccggtcactt
    27481 cttccagcgt acggttggtg tgcagaatga tgttgccgtt ctccacttta tccatcagac
    27541 gcttaatgag gattttttcc gcgcggaaac cgtcacggcg gtgaatcaga tgcacttccg
    27601 aagcgatgtt agacagatac agcgcctctt caaccgcggt attgccgccg ccgatgaccg
    27661 caactttctg gttgcgatag aagaaaccgt cgcaggttgc acaagcagaa actccacggc
    27721 ctttaaaggc ttcttcagag ggcaggccga gatagcgtgc agaagctccg gtggcaataa
    27781 tcagcgcgtc gcaagtgtat tcgccgttat cgccattcag acggaacgga cggttttgca
    27841 gatccacctt gttgatatga tcaaaaatga tctcagtttc aaacttggtg gcatgttcgt
    27901 gcatgcgctc cattaataac ggaccggtca gatcgtttgg atcgccaggc cagttttcca
    27961 cttccgtggt ggtggtcagt tggccgcctt tttccatgcc ggtaatcagc acaggttgca
    28021 ggttggcgcg cgccgcatag acagcagcgg tgtatcccgc cgggcctgaa cccaggataa
    28081 gcagtttact gtgtttggtc gtgcccatga gatccccata gttgttggca gacaatgggc
    28141 aggattgtag ggaatttaca gacgtaaaaa aagagtatga cgattttgtt aacaatttgt
    28201 gcaatcggca gcatcgataa gcaggtcaaa ttctcccgtc attatcacct ctgctactta
    28261 aatttcccgc tttataagcc gattaaatga tgaataaacg cccctgttaa tgaatatctg
    28321 gcatgttgta ctaaaaatcg atgttttgct ttgacaatcc cctggtgttt tgcgaaaaca
    28381 ttcgaggaag aaaaaaaaca gtattcttat atgcgcataa ccatgcatgt aaataccatg
    28441 tttaccgtgc tagtgaaatc tacgtatggc gtggacagac gccattcgtg atgtcgatag
    28501 ctgccacaag gcaacggtct tctcaccgta gacccaggca ttgcgcgccg tgaatcttca
    28561 tgatttcggt ctatcgtgac gggtagcgac tctgaacagt gatgtttcag ggtcagacag
    28621 gagtagggaa ggaatacaga gagacaataa taatggtaga tagcaagaag cgccctggca
    28681 aagatctcga ccgtatcgat cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt
    28741 ctaacgtcga gctttctaaa cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc
    28801 gtcggctgga aagacaaggg tttattcagg gctatacggc gctgcttaac ccccattatc
    28861 tggatgcatc acttctggta ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt
    28921 ttgaacaatt caataccgct gtacaaaaac ttgaagaaat tcaggagtgt catttagtat
    28981 ctggtgattt cgactacctg ttgaaaacac gcgtgccgga tatgtcagcc taccgtaagt
    29041 tgctggggga aaccctgctg cgtctgcctg gcgtcaatga cacacggaca tacgttgtta
    29101 tggaagaagt caagcagagt aatcgtctgg ttattaagac gcgctaacac ggaacaggtg
    29161 caaaatcggc gtattttgat tacactcctg ttaatccata cagcaacagt actggggtaa
    29221 cctggtactg ttgtccgttt tagcatcggg caggaaaagc ctgtaacctg gagagccttt
    29281 cttgagccag gaatacactg aagacaaaga agtcacattg acaaagttaa gtagcggccg
    29341 ccgccttctg gaggcgttgc tgatccttat tgtcctgttt gccgtctggt tgatggctgc
    29401 cttactaagc tttaaccctt cggaccccag ctggtcgcaa acggcctggc atgaacctat
    29461 ccataattta ggtgggatgc ccggtgcgtg gttggcagat acgctgttct ttatttttgg
    29521 cgtgatggct tacaccattc ccgtcattat tgtcggcggt tgttggtttg cctggcgtca
    29581 tcagtccagc gacgaaaaca ttgattattt tgccgtttcg ctacgcatca ttggcgtttt
    29641 ggcgctcatc cttacctcct gtggtctggc ggcaatcaac gctgacgata tctggtattt
    29701 tgcctccggt ggcgtcattg gcagcttact aagcactacg ctacaaccac tgctacacag
    29761 tagcggggga actattgcgc tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg
    29821 ttggtcatgg gtgaccattg ctgaaaaact cggcggctgg attttaaaca ttctcacctt
    29881 cgccagtaat cgtacccgtc gcgatgatac ctgggtcgat gaagatgagt atgaagacga
    29941 cgaagagtat gaagatgaaa accacggcaa acagcatgaa tcacgccgtg cccgtattct
    30001 tcgcggcgcg ctagcgcgtc gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg
    30061 gcaaacagac gctgcgttgt tctccggtaa gcggatggat gatgacgaag agattaccta
    30121 cactgcacgc ggtgtggctg ccgacccgga cgacgtccta ttttcgggca atcgtgcaac
    30181 gcagccagaa tatgacgaat acgatccatt attaaacggt gcgccaatta ccgaacctgt
    30241 cgctgtagca gctgctgcta ccacggcgac acaaagctgg gctgcgccgg ttgaacctgt
    30301 gactcagacg ccgcctgttg cctctgttga tgttccacct tcgcaaccta cagtagcctg
    30361 gcagcctgta ccgggtccac aaacgggaga gccggttatt gctcctgcac cggaaggtta
    30421 cccacagcag tcacaatatg cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc
    30481 agtacagccg cagcagccgt attatgcacc tgcagctgaa caacctgcgc aacagccgta
    30541 ttatgcccct gcgccagaac aaccggtggc aggtaacgcc tggcaagccg aagagcagca
    30601 atccactttt gctccacagt ctacatacca gactgagcaa acttatcagc agccagccgc
    30661 tcaggagccg ttgtaccaac agccgcaacc cgttgaacag cagcctgttg tggagcctga
    30721 acccgttgta gaagagacaa aacccgcgcg tccgccgctt tactactttg aagaagttga
    30781 agagaagcga gcccgtgaac gtgaacaact tgcggcctgg tatcaaccga ttccagaacc
    30841 ggttaaagaa ccagaaccga tcaaatcttc gctgaaagca ccttctgttg cagcagtacc
    30901 tccagtagaa gccgctgccg ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact
    30961 ggcgacgggg gctgccgcaa ccgttgccgc gccagtcttc agtctggcaa atagcggtgg
    31021 accgcgtccg caggtcaaag aggggattgg cccgcagttg ccacgaccga aacgtatccg
    31081 cgtgccaact cgtcgtgaat tggcgtctta cggtattaag ctgccctcac agcgtgcggc
    31141 ggaagaaaaa gcccgtgaag cccagcgcaa tcagtacgat tctggcgatc agtacaacga
    31201 tgatgaaatc gatgcgatgc agcaggatga actggcacgt cagttcgccc agacacagca
    31261 gcaacgctat ggcgaacagt atcaacatga tgtgcccgta aatgcagaag atgcagatgc
    31321 tgcggcagag gctgaactgg ctcgtcagtt tgcgcaaact caacaacaac gttattccgg
    31381 cgaacaaccg gctggggcga atccgttctc gctggatgat tttgaatttt cgccaatgaa
    31441 agcgttgctg gatgatggtc cacacgaacc gctgtttacg ccaattgttg aacctgtaca
    31501 gcagccgcaa caaccggttg caccgcagca gcagtatcag cagccacaac agccggttgc
    31561 gccacagccg cagtatcagc agccacaaca gcaggttgcg ccgcagccac aatatcagca
    31621 gccgcaacaa ccggttgcgc cacagcagca atatcagcag ccgcaacaac cagttgcgcc
    31681 gcagccgcag tatcaacagc cgcaacaacc ggttgcaccg cagccgcagt atcagcagcc
    31741 acaacagcca gttgcgccac agccgcagga tactctgctt catccgctgt tgatgcgtaa
    31801 tggcgacagc cgtccgttgc ataaaccgac gacgccgctg ccttctctgg atttgctgac
    31861 accgccgccg agcgaagtgg agccggtaga tacctttgcg cttgaacaaa tggctcgcct
    31921 ggtggaagcg cgtctggctg atttccgtat taaagccgat gtcgtcaatt actctccggg
    31981 gccggttatc actcgctttg aacttaacct ggcaccgggc gtaaaagcgg cgcgcatttc
    32041 taacttgtca cgggaccttg cccgttcact ttcgacggtg gcggtgcgtg tcgttgaagt
    32101 tattcctggc aaaccctatg taggtctgga gttaccgaat aaaaaacgac aaaccgttta
    32161 tctgcgcgaa gtattggata acgccaaatt ccgcgataat ccatcgccat taaccgtggt
    32221 gctgggtaaa gatatcgccg gtgagccggt ggttgccgat ctggcgaaaa tgccgcactt
    32281 gttagttgcg gggactaccg gttccggtaa atctgtcggt gtgaacgcga tgatcctgag
    32341 catgctttat aaagcacagc cagaagatgt gcgtttcatc atgatcgacc cgaaaatgct
    32401 ggagctttcg gtgtatgaag gcattccgca cctgttaacg gaagtcgtta ctgatatgaa
    32461 agatgccgcc aacgcgctgc gctggtgtgt taacgagatg gagcgtcgtt ataaactgat
    32521 gtctgcgctg ggtgtgcgta atctggcggg ttataacgaa aaaattgctg aagccgatcg
    32581 catgatgcgt ccgattccag acccgtactg gaagccgggt gacagtatgg atgcccagca
    32641 tccggtgctg aaaaaagaac catacattgt ggtgttggtg gacgaatttg ccgacctgat
    32701 gatgacagta ggtaaaaaag tggaagagct gatagcacgt ctggcgcaaa aagcccgtgc
    32761 cgcgggtatc cacctcgtac tggcaactca gcgtccatcg gttgatgtta ttactggtct
    32821 gattaaagcg aatattccga cccgtatcgc ctttaccgta tccagtaaga ttgactcacg
    32881 taccattctt gatcaggctg gcgcggaatc actgctgggt atgggggata tgctctactc
    32941 tgggccgaac tccacgttgc cggtacgtgt ccatggtgct tttgttcgcg atcaggaagt
    33001 tcatgccgtg gtgcaggact ggaaagcgcg tggtcgccca cagtatgttg atggcatcac
    33061 ctccgacagc gaaagcgaag gtggtgcggg tggtttcgat ggcgctgaag aactggatcc
    33121 gttgttcgat caggcggtgc agtttgtcac tgaaaaacgc aaagcgtcaa tttctggcgt
    33181 acaacgtcag ttccgcattg gttataaccg tgcagcgcgt attatcgaac agatggaagc
    33241 gcaggggatt gtcagcgaac aggggcacaa cggtaatcgt gaagtgctgt ccccaccgcc
    33301 gtttgactaa ttaatgcatt gccggataag gcgcggtagc gtcgcatccg gcactctatc
    33361 aactgaaaat tcagtatttt cttctttcct caagctgatt attagcctgg aatagagagt
    33421 agagggaact cccgatcggg agtgacgtaa tttgaggaat aatgatgaaa aaaattgcca
    33481 tcacctgtgc attactctca agcttagtag caagcagcgt ttgggctgat gccgcaagcg
    33541 atctgaaaag ccgcctggat aaagtcagca gcttccacgc cagcttcaca caaaaagtga
    33601 ctgacggtag cggcgcggcg gtgcaggaag gtcagggcga tctgtgggtg aaacgtccaa
    33661 acttattcaa ctggcatatg acacaacctg atgaaagcat tctggtttct gacggtaaaa
    33721 cactgtggtt ctataacccg ttcgttgagc aagctacggc aacctggctg aaagatgcca
    33781 ccggtaatac gccgtttatg ctgattgccc gcaaccagtc cagcgactgg cagcagtaca
    33841 atatcaaaca gaatggcgat gactttgtcc tgacgccgaa agccagcaat ggcaatctga
    33901 agcagttcac cattaacgtg ggacgtgatg gcacaatcca tcagtttagc gcggtggagc
    33961 aggacgatca gcgcagcagt tatcaactga aatcccagca aaatggggct gtggatgcag
    34021 cgaaatttac cttcaccccg ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc
    34081 acctgagtga gcaatctgtc gctcgatttt tcggataata cttttcaacc tctggccgcg
    34141 cgtatgcggc cagaaaattt agcacagtat atcggtcagc aacatttgct ggctgcgggg
    34201 aagccgttgc cgcgcgctat cgaagccggg catttacatt ctatgatcct ctgggggcca
    34261 ccgggtaccg gcaaaacaac cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg
    34321 gaacgtattt ctgccgtcac ctctggtgtg aaagagattc gcgaggcgat cgagcgcgcc
    34381 cggcaaaacc gcaatgcagg tcgccgcact attctttttg ttgacgaagt tcaccgtttc
    34441 aacaaaagcc agcaggatgc atttctgcca catattgaag acggcaccat cacttttatt
    34501 ggcgcaacca ctgaaaaccc gtcgtttgag cttaattcgg cactgctttc ccgtgcccgt
    34561 gtttatctgt tgaaatccct gagtacagag gatattgagc aagtactaac tcaggcgatg
    34621 gaagacaaaa cccgtggcta tggtggtcag gatattgttc tgccagatga aacacgacgt
    34681 gccattgcag aactggtgaa tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg
    34741 gcggatatgg ccgaagtcga tgatagcggt aagcgggtcc tgaagcctga attactgacc
    34801 gaaatcgccg gtgaacgtag cgcccgcttt gataacaaag gcgatcgctt ttacgatctg
    34861 atttccgcac tgcataagtc ggtacgtggt agcgcacccg atgcggcgct gtactggtat
    34921 gcgcgaatta ttaccgctgg tggcgatccg ttatatgtcg cgcgtcgctg tctggcgatt
    34981 gcgtctgaag acgtcggtaa tgccgatcca cgggcgatgc aggtggcaat tgcggcctgg
    35041 gattgcttta ctcgcgttgg cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt
    35101 tacctggcct gcgcgccaaa aagcaacgct gtctacactg cgtttaaagc cgcgctggcc
    35161 gatgctcgcg aacgcccgga ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa
    35221 ttaatgaagg aaatgggcta cgggcaggaa tatcgttacg ctcatgatga agcaaacgct
    35281 tatgctgccg gtgaggttta cttcccgccg gaaatagcac aaacacgcta ttatttcccg
    35341 acaaacaggg gccttgaagg caagattggc gaaaagctcg cctggctggc tgaacaggat
    35401 caaaatagcc ccataaaacg ctaccgttaa tgttatcgtt gcggtaatgt tgttactgta
    35461 tccctgtggt cgcaggctgt ggccacatct cccatttaat tcgataagca caggataagc
    35521 atgctcgatc ccaatctgct gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc
    35581 cggggcttta agctggatgt agataagctg ggcgctcttg aagagcgtcg taaagtattg
    35641 caggtcaaaa cggaaaacct gcaagcggag cgtaactccc gatcgaaatc cattggccag
    35701 gcgaaagcgc gcggggaaga tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa
    35761 gagctggatg cagcaaaagc cgagctggat gctttacagg ctgaaattcg cgatatcgcg
    35821 ctgaccatcc ctaacctgcc tgcagatgaa gtgccggtag gtaaagacga aaatgacaac
    35881 gttgaagtca gccgctgggg taccccgcgt gagtttgact ttgaagttcg tgaccacgtg
    35941 acgctgggtg aaatgcactc tggcctcgac tttgcagctg cagttaagct gactggttcc
    36001 cgctttgtgg taatgaaagg gcagattgct cgcatgcacc gcgcactgtc gcagtttatg
    36061 ctggatctgc ataccgaaca gcatggctac agtgagaact atgttccgta cctggttaac
    36121 caggacacgc tgtacggtac gggtcagctg ccgaaatttg ctggcgatct gttccatact
    36181 cgtccgctgg aagaagaagc agacaccagt aactatgcgc tgatcccaac tgcagaagtt
    36241 ccgctgacta acctggtgcg cggtgaaatc atcgatgaag atgatctgcc aattaagatg
    36301 accgcccaca ccccatgctt ccgttctgaa gccggttcat atggtcgtga cacccgtggt
    36361 ctgatccgta tgcaccagtt cgacaaagtt gaaatggtgc agatcgtgcg cccggaagac
    36421 tcaatggcgg cgctggaaga gatgactggt catgcagaaa aagtcctgca gttgctgggc
    36481 ctgccgtacc gtaaaatcat cctttgcact ggcgacatgg gctttggcgc ttgcaaaact
    36541 tacgacctgg aagtatggat cccggcacag aacacctacc gtgagatctc ttcctgctcc
    36601 aacgtttggg atttccaggc acgtcgtatg caggcacgtt gccgcagcaa gtcggacaag
    36661 aaaacccgtc tggttcatac cctgaacggt tctggtctgg ctgttggtcg tacgctggtt
    36721 gcagtaatgg aaaactatca gcaagctgat ggtcgtattg aagtaccaga agttctgcgt
    36781 ccgtatatga acggactgga atatattggc taatacccaa tttttctgaa tctaaaaagc
    36841 gcctacgggc gctttttttg tctccctttg ataccgaaca ataattactc ctcacttaca
    36901 cgtaatacta ctttcgagtg aaaatctacc tatctctttg attttcaaat tattcgatgt
    36961 atacaagcct atatagcgat ctgctataga aataattaca caatacggtt tgttactgga
    37021 atcaatcgtg agcaagcttg agtgagccat tatgaaaacg aaaatccctg atgcggtatt
    37081 ggctgctgag gtgagtcgcc gtggtttggt aaaaacgaca gcgatcggcg gcctggcaat
    37141 ggccagcagc gcattaacat taccttttag tcggattgcg cacgctgtcg atagcgccat
    37201 tccaacaaaa tcaggcgaaa aggttatctg gagcgcctgt acagttaact gtggtagtcg
    37261 ctgcccgcta cgtatgcacg tcgtggacgg tgaaatcaaa tatgtcgaaa cggacaatac
    37321 cggcgatgac aattacgacg gcctgcacca ggttcgcgcc tgcctgcgtg ggcgttccat
    37381 gcgtcgccgt gtctacaatc cggaccgcct gaaatatccg atgaaacgag tcggggcgcg
    37441 cggtgaaggc aaattcgagc gcattagctg ggaagaagcc tacgacatca tcgcgaccaa
    37501 tatgcagcgc ctgatcaaag agtacggcaa cgaatccatc tatctgaact atggcaccgg
    37561 tacgctgggt ggcaccatga cccgctcctg gccgccggga aataccctgg tcgcgcggct
    37621 gatgaactgc tgcggcggct atctgaacca ttacggcgac tactcctccg cgcaaattgc
    37681 ggaaggtctg aactatacct acggcggctg ggcagatggc aacagcccgt cggatatcga
    37741 aaacagtaag ctggtagtgc tgtttggtaa taaccctggc gaaacgcgaa tgagtggcgg
    37801 tggggtgact tactatcttg aacaggcacg ccagaaatct aatgcccgca tgatcatcat
    37861 cgatccgcgc tataccgaca ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc
    37921 gggaacagat gccgcactgg ttaacggtct ggcgtacgtc atgatcactg aaaacctggt
    37981 ggatcaggca ttcctcgata aatattgcgt tggctacgat gagaaaaccc tgccagccag
    38041 tgcgccgaaa aatggccact ataaagctta tattctgggt gaagggccag atggcgtggc
    38101 taaaacaccg gaatgggcct cgcaaatcac cggtgttccg gcagacaaaa tcatcaaact
    38161 ggctcgtgaa atcggcagta ccaaaccggc gtttatcagc cagggatggg gcccgcagcg
    38221 tcacgctaac ggtgaaatcg caacccgtgc tatctcgatg ctggcgattc tgaccggtaa
    38281 cgttggtatt aatggaggga acagcggcgc gcgtgaaggt tcatacagtt taccgtttgt
    38341 ccgtatgccg accttggaaa acccgatcca gaccagcatt tcgatgttta tgtggaccga
    38401 tgccattgaa cgtggcccgg aaatgacggc gctacgtgat ggtgtgcgcg ggaaagataa
    38461 gctggatgtg ccgatcaaaa tgatctggaa ctatgccggt aactgcctga ttaaccagca
    38521 ttctgaaatc aaccgtaccc atgaaatcct tcaggatgat aagaagtgcg agctgattgt
    38581 ggttatcgac tgccacatga cctcatcggc gaaatatgct gacatcctgc tgcctgactg
    38641 caccgcttcc gaacagatgg actttgcact ggatgcatcc tgcgggaata tgtcttacgt
    38701 gattttcaac gatcaggtga ttaaaccgcg ctttgaatgt aagaccatct atgaaatgac
    38761 cagcgaactg gcaaaacgtc ttggcgttga gcaacagttt actgaaggcc gtacccagga
    38821 agagtggatg cggcatctgt atgcccagtc gcgggaagcg attcctgaac tgccaacgtt
    38881 tgaagagttc cgcaagcagg ggatctttaa aaagcgcgac ccacaagggc atcacgttgc
    38941 ttataaagcc ttccgtgaag atccgcaggc aaatccactg accacgccat cgggtaaaat
    39001 tgagatttat tcgcaggcgc tggctgacat tgccgctacc tgggaattgc cagaaggcga
    39061 tgtgatcgat ccactgccga tctacacgcc gggctttgaa agttatcagg atccgctgaa
    39121 caaacagtat ccgctgcagc ttacaggttt ccactataaa tctcgcgttc actcaactta
    39181 cggcaacgtt gatgtgctga aagcggcttg ccgtcaggaa atgtggatca acccgcttga
    39241 tgcccaaaaa cgcggtatcc acaacggcga taaagtcagg atctttaacg atcgtggtga
    39301 ggttcatatt gaggcgaaag tgacgccacg aatgatgccg ggtgtggtcg cactgggtga
    39361 aggtgcctgg tatgacccgg atgcaaaacg tgtcgataag ggtggttgta ttaacgtact
    39421 gaccactcaa cgtccgtctc ctctcgctaa ggggaatccg tcacatacaa accttgttca
    39481 ggttgaaaag gtgtaaggag taaccgatga caacccagta tggatttttt attgattcca
    39541 gccgttgcac cggttgcaaa acctgcgagc tggcttgtaa agactacaaa gatttgatgc
    39601 cagaagtcag cttccgccgc atttatgaat atgctggtgg cgactggcag gaagataacg
    39661 gtgtctggca ccagaacgtg tttgcctact atctgtcgat ttcgtgcaac cactgcgaag
    39721 atccggcttg tactaaagtc tgcccgagcg gtgcgatgca taaacgtgaa gatggttttg
    39781 ttgtggtcga tgaagatgtg tgcattggct gccgctactg ccatatggct tgcccgtatg
    39841 gcgcaccgca atataacgaa acgaaaggcc atatgaccaa atgcgatggt tgttatgacc
    39901 gtgttgcgga gggtaaaaag ccgatctgtg ttgaatcttg tccgctgcgg gcgctggatt
    39961 tcggccctat cgacgagctg cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc
    40021 cgcgagctca ctttaccaaa ccgaatattg tgatcaaacc caatgccaat agccgcccga
    40081 ccggggatac cactggctat ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg
    40141 catgaatggc cgctgatgat cttcacggtc ttcgggcaat gtgtggcggg tggttttatc
    40201 gttctggctt tggcgctgct taaaggcgac ctgcgagcag aagcccagca gcgtgttatc
    40261 gcctgcatgt ttggcttatg ggtgctgatg ggcattggct ttatcgcctc tatgctccat
    40321 cttggttcac cgatgcgcgc ctttaactcg ctcaaccggg taggggcttc agcactcagt
    40381 aacgaaatcg ccagcggttc gatctttttt gctgtaggcg gcatcggctg gttgctggca
    40441 atgctgaaaa agctgtcacc tgcattgcgt acgctatggc tggtggtgac gatggttctt
    40501 ggcgtcgtct ttgtctggat gatggtgcgt gtgtataaca gcattgatac cgttccgacc
    40561 tggtacagca tctggacgcc gatgggcttc ttcctgacga tgttcatggg cggcccgtta
    40621 ctgggttacc tgttgctgag tctggctggc gtcgatggct gggcgatgcg tctgctgcca
    40681 gccatttctg tactggcact ggtggtgagt ggcgtgatgt cagtgatgca gggcacagag
    40741 ctggcaacta ttcatagttc tgtgcagcag gccgcagcgc tggtgccgga ctatggtgcg
    40801 ctgatgtcct ggcgaatcgt gcttttggcc gttgccctgt gcttgtggat tgcaccacag
    40861 ctaaaaggtt atcagcctgc ggttccgcta ctatccgtct cattcattct gcttctggca
    40921 ggggaattaa tcggtcgcgg cgtattctat ggtttgcata tgaccgtggg gatggccgtc
    40981 gcaagctaat cagtaacaac cggggtttcg gccccggttc tcttttattt ctgcttcgtt
    41041 aacgtgtcat aactggtcat caaattacga taatcaggaa tgtggttaga gaacaacgtc
    41101 gccagtcctt caatatcatt acgccagtca cggtgcagct cgcaagccac gccgaaccat
    41161 gtcatcaatt gcgcacctgc ttgcgacatg cgatcccatg ccgaatgccg ggtaatttca
    41221 ttaaaggtcc ctgacgcgtc agtcacaaca aagacatcaa acccttcttc aatggctgaa
    41281 agcgccggga atgccacgca aacttcggtt accacaccgg caataattaa ctgtttttta
    41341 cctgtcgctt tgactgcttt tacaaaatct tcgttatccc aggcgttaat attgccaggg
    41401 cgagcaatgt aaggtgcatc ggggaattgt gctttcagct ctggaactaa tgggccgtta
    41461 ggaccggttt caaaactggt ggtaagaata gttggtaagt tgaaatactt agccaggtca
    41521 cccagcgcca gcacgttatt tttaaactta tcgggttcga tatcccgtac aagggaaagt
    41581 aaaccagcct ggtgatcaac aagcaaaacg gcagcatcat ttttatcaag acgaacatac
    41641 ggtttggtca tcctcttctc ctttcgagag tcagtggtac gtctgaaatc ggtcagacgc
    41701 gatgcattgc tctgaaagca tagacgggaa atatgagttt gctgtgacca tgaaattttt
    41761 cgactgaacg aagcgtatga aatttgtgtt agatcaataa aaacaatcag atgagattaa
    41821 tcatctcttc agcattagcg ttatgaatga gtaatataaa tcgataatac cgccgctgcg
    41881 atatgcgcgt attttcaccc attgacaatg ttttttgcgg tggcatgatg cgcatgaaat
    41941 ttgaacttcc tcacggtttt aattcatgtc cacgtatacc cggcctgtca tgcttttgct
    42001 gtctggcctg cttttgttga ctctggcgat cgcggtgtta aatacactcg tgccgctttg
    42061 gctcgcccag gaacacatgt ccacatggca ggtaggcgtt gtcagctcat cctattttac
    42121 cggcaacctt gtcggtacat tgctgacagg gtatgtcatt aaacgcattg gctttaaccg
    42181 cagctattat ctggcctcct tcatttttgc cgctggctgt gccggccttg gcctgatgat
    42241 tggtttctgg agctggttgg cgtggcgttt tgtcgcgggc gtcggctgtg ccatgatttg
    42301 ggtagtcgtt gagagcgcgc tgatgtgcag tgggacgtca cgtaaccgtg ggcgtttgct
    42361 tgctgcgtat atgatggttt attacgtggg aacattttta ggccagttac tggtcagcaa
    42421 agtctcaacc gagctgatga gcgtattgcc gtgggttaca ggtttgacgt tggcagggat
    42481 cttaccgctg ttgtttacgc gtgtcctgaa tcagcaggct gaaaaccatg attcgacgtc
    42541 aattacgtca atgctaaaac tccgtcaggc gcggcttggc gtgaatggct gcattatctc
    42601 aggaatcgtg ctgggatctc tatatggcct gatgccgctg tacctcaatc acaaaggggt
    42661 gagcaatgcc agcattggtt tctggatggc ggtactggtc agtgcgggta tcctcggaca
    42721 atggccgatt ggacgtctgg cggataagtt tggtcgactg ctggtgttgc gtgttcaggt
    42781 ctttgtcgtc attctcggca gtatcgcgat gcttagccag gcggcgatgg ccccagcgtt
    42841 attcatcctc ggtgccgctg gctttacgct atatccggtg gcgatggcct gggcttgtga
    42901 gaaagttgaa catcatcaac tggtggcgat gaaccaggcc ttactgttga gctataccgt
    42961 gggaagtctg cttggcccgt catttaccgc tatgctaatg cagaatttct ccgataattt
    43021 attgtttatc atgatcgcca gcgtatcgtt tatctatttg ctgatgctgc ttcgcaacgc
    43081 cggtcatacg ccgaaacccg ttgctcacgt gtaaatgaat tcaagcaaag tgtgaactta
    43141 ctgtttcaca ctctgctttt ttgtttcttc tatctgactt gcttttttcc aaattttatt
    43201 cgtttaaaaa taaaatgtgc agcaggttat aattttgcat ttcgctattt ccgcacttct
    43261 tatttgccgc gcataatccc tcgttttaca gatgcccctt taattttggc gaaggatttg
    43321 tctatggctg ggaatgttca ggaaaaacag ctgcgatggt acaacattgc gctgatgtct
    43381 tttatcactg tctggggttt tggcaacgtt gttaataact atgccaacca ggggctggtg
    43441 gttgtttttt catgggtgtt tatctttgcg ctctatttca caccttatgc actaattgtt
    43501 ggtcagttag gctcgacctt caaagatggg aagggcgggg tcagtacctg gattaaacac
    43561 acgatgggac ccggattggc ttacctcgcc gcgtggacct actgggtggt gcatattccc
    43621 tatctggcac aaaaacccca ggcaatactg attgcgctcg gttgggcgat gaaaggcgac
    43681 ggttcgctaa tcaaagaata ttcagtcgta gcgttacagg ggttaacgct ggtgctgttt
    43741 atcttcttta tgtgggttgc ttcacgcggt atgaaatcgc tgaaaatcgt cggttctgtg
    43801 gcagggattg cgatgtttgt tatgtcgctc ctgtatgtgg cgatggcggt aaccgcgcct
    43861 gcgattactg aagtgcatat tgcgaccaca aacattacct gggaaacgtt cattcctcat
    43921 atcgacttta cctacattac cactatttca atgctggttt tcgcggttgg cggagctgag
    43981 aagatttctc cttacgttaa tcaaacgcgc aacccaggaa aagaatttcc aaaagggatg
    44041 ttatgcctgg cggtgatggt tgcggtttgt gccattctgg gctcactggc gatggggatg
    44101 atgtttgact cgcgtaatat cccggatgac ttaatgacta acggtcagta ttacgccttt
    44161 cagaagctgg gcgagtatta caacatgggt aatactttaa tggtgattta cgccattgcg
    44221 aataccctgg gacaagtagc tgcgctggta ttctcgattg atgccccgct taaagtgcta
    44281 ttaggcgatg ctgacagcaa atatattcca gccagtttat gtcgtaccaa cgcttctggt
    44341 acgcccgtta atggctattt tctgaccctg gtgctggtgg cgatcctgat tatgctgccg
    44401 actctcggca tcggtgatat gaacaatctc tataaatggc tgttgaatct taactcggta
    44461 gtgatgccgc tgcgttattt atgggtattt gttgcattta ttgcagtcgt tcgcctggcg
    44521 cagaaatata caccagagta tgtctttatt cgtaataagc ctctggcaat gaccgtcggg
    44581 atttggtgtt ttgcctttac tgcctttgcc tgtttgacgg ggatcttccc gaaaatggaa
    44641 gccttcactg cagagtggac cttccagttg gcgctgaatg ttgcaacgcc gtttgtgctg
    44701 gtaggattag gactgatatt cccgctgctg gcgcgtaaag cgaatagtaa ataattattg
    44761 tggtcggtga cgctgactct ggcagatctg acccgttttt acataatcga gaaatgcgcg
    44821 caagccgcag gacatatact ggcggtttgg gtaatacatt tggaagctgt gctgtaaggg
    44881 cgttagaaat agagctctga attatttatg cagcgcggaa taaaaaagcg gcaactcaat
    44941 aaagttgccg ctttacgggg aaattagaac attaccttat gaccgtactg ctcaagaata
    45001 cctttcacgc gttccatggt ttctttcttc ggtggtttaa caccatcgag tttgtattct
    45061 tcacccatcg ccacccattt gtgtttgccc agttcgtggt aggggagaag ttcgattttc
    45121 tcaacgttac ccatatcacg ggtaaattca ccgaggcgat gcgctgaatc gtcatcgtca
    45181 gaccagcctg ggacaacaac gtagcggatc cacaccttca catttttatt cgccagatat
    45241 ttggcgaact ccagcgtgcg gtggttggaa actccaacca gattttggtg gatctcgtcg
    45301 ttcatctgtt tgagatcgag cattaccagg tcggttactt ccagcagttc atcaatcacc
    45361 ggatcgtaac gacgaacaaa accgttggtg tccagacagg tatgaatgcc ttcttttttg
    45421 caggcgcgga accagtcacg aacaaactca gcttgcagga ttgcttcacc gccggatgcg
    45481 gtaacgccgc cgccggaagc gttcataaag tggcgatagg tcaccacttc cttcatcaaa
    45541 tcttcaacgg taacttcttt accgccatgc gtgtcccagg tgtcgcggtt atgacaatac
    45601 aggcagcgca tcaggcagcc ctggaaaaag gtgataaagc gaatacctgg gccgtctacg
    45661 gttccacagg attcaaagga gtgaatgcga ccaataactg acattgcggt gtttctccag
    45721 atgtggccca tctgaggccg tgttggtgcg cagctcgaag gctacgtcga gtctgttttg
    45781 gcagtcacct taaagtatag atagctgaca aaaaaggctc acgtgctaaa aaaggcccca
    45841 ctttcgtgga gcctttattg tacgcttttt actgtacgat ttcagtcaaa tctaattaca
    45901 tagattgagt gaaggtacga gtaataacgt cctgctgctg ttctttagtc agcgagttga
    45961 aacgtactgc gtagccagat acacggatgg tcagctgcgg atatttttcc ggattttcca
    46021 tcgcgtcgag cagcatttca cggttcatca cgttaacgtt caggtgctga ccaccttcga
    46081 tggatgcttc gtggtggaag taaccatcca tcagaccagc caggttggtc ttacgaactt
    46141 cgtcgtcttt acccagtgcg ttcggaacga tagagaaggt gtaggagata ccatctttag
    46201 cgtaagcaaa cggcagttta gcaacggaag tcagagacgc tacagcacct ttctggtcac
    46261 gaccgtgcat cgggttagca cccggtccga acggcgcgcc agcacgacga ccgtctgggg
    46321 tgttacccgt tttcttacca tacacaacgt tagaagtgat ggtcagaaca gactgagtcg
    46381 ggatagcgtc acggtaggtg tgcagtttct gaattttctt catgaaacgt tctaccaggt
    46441 caacagccag gtcatctaca cgcggatcgt tgttaccaaa ctgcgggtat tcgccttcga
    46501 tttcgaagtc gatagccaga ccgtcttcgt cacgaatcgg tttaactttc gcatatttga
    46561 ttgcagacag ggagtcagca gcaacggaca gaccagcgat accacacgcc atggtgcgga
    46621 taacgtcacg gtcgtgcagc gccatcagag aggcttcgta gctgtacttg tcgtgcatgt
    46681 agtgaatgat gttcagtgca gtgatgtact gtttagccag ccagtccatg aagtgatcca
    46741 tgcgctccat cacttcatcg tagttcagga gatcgccttt gatcggttca gacttcggac
    46801 caacctgcat tttcagtttt tcgtcaacgc cgccgttgat tgcgtacagc atggttttcg
    46861 ccaggtttgc acgcgcaccg aagaactgca tttgtttacc aacgatcatc gggcttacgc
    46921 agcaagcgat agcgtagtca tcgttgttga agtccggacg catcaggtca tcgttctcat
    46981 actgcagaga agaggtgtcg atggacactt tagcggcgaa tttcttgaag ttcagcggca
    47041 gtttttcaga ccacagaatg gtcatgttcg gttccggaga cggacccatg gtgtacaggg
    47101 tgttcaggaa acggaagctg tttttggtaa ccagggtacg accgtcgagg cccataccac
    47161 cgatagattc ggttgcccag atcgggtcgc cagagaacag ttcatcgtac tccggagtac
    47221 gcaggaagcg aaccatacgc agtttcatga ccaggtggtc aaccatttcc tgcgcttctt
    47281 gttcggtgat cttgccagct ttcaggtcac gttcgatgta cacatccagg aaggtggagg
    47341 tacgaccgaa ggacattgca gcaccgttct gagacttaac agcagccagg tagccgaagt
    47401 aagtccactg gatagcttcc tgagcgttgg tagccggacc agagatgtcg tagccgtatt
    47461 tcgcagccat ttctttcatc tgacccagag cgcggtgctg ttcagcgatt tcttcgcgca
    47521 gacggatagt ctgttccagg tttacgccgt tttccagatc agcctgcaga gaggtgaact
    47581 gagcgtattt gtctttcatc aggtagtcga taccgtacag cgcaacgcga cggtagtcac
    47641 cgatgatacg gccacggcca taagcatctg gcagaccggt cagaacaccg gatttacggc
    47701 aacgcaggat gtccggagtg taaacgtcga acacgccctg gttgtgagtt ttacggtatt
    47761 cagtgaagat ttttttgatc atcgggtcca gttcgcggtt gtacgctttg caggaacctt
    47821 cgatcatttt gataccaccg aacgggataa gagcacgttt cagcggagct tcagtctgca
    47881 gaccaacaac tttttccaac gctttgttga tgtagccagc gtcgtgagag gtgatggtgg
    47941 aagcaacagc ggtgtcgaag tcaactggcg cgtgagtgcg gttttccagt ttaacgcctt
    48001 ccattacttt gtcccacagg gtggtggtcg cttcagtagc gccagccagg aaggactcgt
    48061 caccctcgta cggagtgtag tttttctgaa tgaagtcacg gacgtttact tcattctgcc
    48121 agtcaccttt ggtaaaacct tcccaggctg tggctaactt ttcattaagc tcggacatgt
    48181 aacacctacc ttcttaagtg gattttttat ttactgcgta cttcgacaac cattaatggt
    48241 gatcgttttc acgcaggtaa atgacccagt atgtcaaccc aaccaacaaa ccaccgccga
    48301 taatattacc gatcgtaacc ggaatcaggt tatcagtgat gaagttcatc acggtcaggt
    48361 gagaaaaatt ttccggtgca gaaccgacag cggtccagaa ttccggagat gcgaagtcgc
    48421 ggattacaat acccatcggg atcataaaca tgtttgcgat actgtgctca aaaccgctgg
    48481 caacaaacat cgcgaccggc agcaccatga taaacgcttt gtccatcagg ctgcggccag
    48541 aataactcat ccatactgcc agacatacca tcaggtttgc caggatacca aggcagacgg
    48601 cctcaataaa agtatggtgc actttgtggt cggcggtttg taggacgttt agtccccatt
    48661 gaccatttgc ggtcatatac tcgccggaaa gccacattaa aagtacaaac agcagtgcgc
    48721 cgaccaggtt gccaaaatag acatttagcc agtttttagc caactgaccc caggtgatgc
    48781 gcccactcgc cttagcaaca acaatcaaca ctgtggaagt aaagagatcg gctccgcaga
    48841 caacacaaag aatcagtccc agagagaagc aaatgccgcc aaccagtttt gccatgccga
    48901 agggcattgt gcctgtgcca gtggttgctg tgatatagaa gacgaatgcg attgagatga
    48961 aaacaccggc ggtaatcgcc agatagaaag tcttaagcgg atgtttcgtt gctttataga
    49021 cacccgcctc ttcggccact ttggccattg cagcaggaag taaaagatca aaagggttgt
    49081 cagctttcac actaactctc tctttattaa gtcggcgacg agatactaac aaagcattat
    49141 agatgagaaa ttgatataga tcatatctcg cctggcttat aggcc
//
LOCUS       DABSYT020000024        47678 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.024, whole genome shotgun sequence.
ACCESSION   DABSYT020000024 DABSYT020000000
VERSION     DABSYT020000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.024"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            213..1547
                     /locus_tag="ID725_RS08855"
                     /old_locus_tag="pgaptmp_001771"
     CDS             213..1547
                     /locus_tag="ID725_RS08855"
                     /old_locus_tag="pgaptmp_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HGV7850391.1"
                     /translation="MTASGTGLGYGEGDESYGYDSCGYLKAQSAGGHRISEETDQYAG
                     GHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEY
                     RHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDELYSVRHLVFNGFELISQQC
                     SRVRQPHPSVAPQWVTRTNHAVNDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPAD
                     TDYPDPRGERDAEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGE
                     QTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTGFLDGVRLSRSQINNIA
                     KEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLYEVYHEVIHAKQFAKIG
                     REAYEALGRLSREEHVLNEILKSKNLFNEAEIAHAIKYVEGLREKFMMGLIN"
     gene            1586..1795
                     /locus_tag="ID725_RS08860"
                     /old_locus_tag="pgaptmp_001772"
     CDS             1586..1795
                     /locus_tag="ID725_RS08860"
                     /old_locus_tag="pgaptmp_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850392.1"
                     /translation="MARRELELREIPYIKNSLHANYSYKSISIGSKQGWLISAKLKVP
                     ETFEPDMIFIEISDPEGFINIPDVL"
     gene            2010..3146
                     /locus_tag="ID725_RS08865"
                     /old_locus_tag="pgaptmp_001773"
     CDS             2010..3146
                     /locus_tag="ID725_RS08865"
                     /old_locus_tag="pgaptmp_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGV7850393.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDLGETHLNFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRRAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     SEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS"
     gene            3412..5649
                     /gene="nrfB"
                     /locus_tag="ID725_RS08870"
                     /old_locus_tag="pgaptmp_001774"
     CDS             3412..5649
                     /gene="nrfB"
                     /locus_tag="ID725_RS08870"
                     /old_locus_tag="pgaptmp_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGV7850394.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            5636..8608
                     /gene="nfrA"
                     /locus_tag="ID725_RS08875"
                     /old_locus_tag="pgaptmp_001775"
     CDS             5636..8608
                     /gene="nfrA"
                     /locus_tag="ID725_RS08875"
                     /old_locus_tag="pgaptmp_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGV7850395.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            8609..9499
                     /gene="ybcH"
                     /locus_tag="ID725_RS08880"
                     /old_locus_tag="pgaptmp_001776"
     CDS             8609..9499
                     /gene="ybcH"
                     /locus_tag="ID725_RS08880"
                     /old_locus_tag="pgaptmp_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGV7850396.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            9682..10443
                     /gene="envY"
                     /locus_tag="ID725_RS08885"
                     /old_locus_tag="pgaptmp_001777"
     CDS             9682..10443
                     /gene="envY"
                     /locus_tag="ID725_RS08885"
                     /old_locus_tag="pgaptmp_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGV7850397.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(10489..10565)
                     /locus_tag="ID725_RS08890"
                     /old_locus_tag="pgaptmp_001778"
     tRNA            complement(10489..10565)
                     /locus_tag="ID725_RS08890"
                     /old_locus_tag="pgaptmp_001778"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10529..10531),aa:Arg,seq:tct)
     gene            10808..11440
                     /gene="fimZ"
                     /locus_tag="ID725_RS08895"
                     /old_locus_tag="pgaptmp_001779"
     CDS             10808..11440
                     /gene="fimZ"
                     /locus_tag="ID725_RS08895"
                     /old_locus_tag="pgaptmp_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGV7850398.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(11443..11958)
                     /gene="sfmF"
                     /locus_tag="ID725_RS08900"
                     /old_locus_tag="pgaptmp_001780"
     CDS             complement(11443..11958)
                     /gene="sfmF"
                     /locus_tag="ID725_RS08900"
                     /old_locus_tag="pgaptmp_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGV7850399.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(11969..12976)
                     /gene="fimH"
                     /locus_tag="ID725_RS08905"
                     /old_locus_tag="pgaptmp_001781"
     CDS             complement(11969..12976)
                     /gene="fimH"
                     /locus_tag="ID725_RS08905"
                     /old_locus_tag="pgaptmp_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGV7850400.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(12989..15598)
                     /gene="sfmD"
                     /locus_tag="ID725_RS08910"
                     /old_locus_tag="pgaptmp_001782"
     CDS             complement(12989..15598)
                     /gene="sfmD"
                     /locus_tag="ID725_RS08910"
                     /old_locus_tag="pgaptmp_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGV7850401.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(15629..16321)
                     /gene="fimC"
                     /locus_tag="ID725_RS08915"
                     /old_locus_tag="pgaptmp_001783"
     CDS             complement(15629..16321)
                     /gene="fimC"
                     /locus_tag="ID725_RS08915"
                     /old_locus_tag="pgaptmp_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGV7850402.1"
                     /translation="MMTKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(16541..17083)
                     /gene="fimA"
                     /locus_tag="ID725_RS08920"
                     /old_locus_tag="pgaptmp_001784"
     CDS             complement(16541..17083)
                     /gene="fimA"
                     /locus_tag="ID725_RS08920"
                     /old_locus_tag="pgaptmp_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGV7850403.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            17563..18429
                     /gene="folD"
                     /locus_tag="ID725_RS08925"
                     /old_locus_tag="pgaptmp_001785"
     CDS             17563..18429
                     /gene="folD"
                     /locus_tag="ID725_RS08925"
                     /old_locus_tag="pgaptmp_001785"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGV7850404.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            18431..18643
                     /gene="ybcJ"
                     /locus_tag="ID725_RS08930"
                     /old_locus_tag="pgaptmp_001786"
     CDS             18431..18643
                     /gene="ybcJ"
                     /locus_tag="ID725_RS08930"
                     /old_locus_tag="pgaptmp_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGV7850405.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            18751..19272
                     /gene="ybcI"
                     /locus_tag="ID725_RS08935"
                     /old_locus_tag="pgaptmp_001787"
     CDS             18751..19272
                     /gene="ybcI"
                     /locus_tag="ID725_RS08935"
                     /old_locus_tag="pgaptmp_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGV7850406.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(19308..20693)
                     /gene="cysS"
                     /locus_tag="ID725_RS08940"
                     /old_locus_tag="pgaptmp_001788"
     CDS             complement(19308..20693)
                     /gene="cysS"
                     /locus_tag="ID725_RS08940"
                     /old_locus_tag="pgaptmp_001788"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGV7850407.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            20867..21361
                     /gene="ppiB"
                     /locus_tag="ID725_RS08945"
                     /old_locus_tag="pgaptmp_001789"
     CDS             20867..21361
                     /gene="ppiB"
                     /locus_tag="ID725_RS08945"
                     /old_locus_tag="pgaptmp_001789"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGV7850408.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            21364..22086
                     /gene="lpxH"
                     /locus_tag="ID725_RS08950"
                     /old_locus_tag="pgaptmp_001790"
     CDS             21364..22086
                     /gene="lpxH"
                     /locus_tag="ID725_RS08950"
                     /old_locus_tag="pgaptmp_001790"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGV7850409.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            22204..22713
                     /gene="purE"
                     /locus_tag="ID725_RS08955"
                     /old_locus_tag="pgaptmp_001791"
     CDS             22204..22713
                     /gene="purE"
                     /locus_tag="ID725_RS08955"
                     /old_locus_tag="pgaptmp_001791"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGV7850410.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            22710..23777
                     /gene="purK"
                     /locus_tag="ID725_RS08960"
                     /old_locus_tag="pgaptmp_001792"
     CDS             22710..23777
                     /gene="purK"
                     /locus_tag="ID725_RS08960"
                     /old_locus_tag="pgaptmp_001792"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGV7850411.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(23916..24809)
                     /gene="ybcF"
                     /locus_tag="ID725_RS08965"
                     /old_locus_tag="pgaptmp_001793"
     CDS             complement(23916..24809)
                     /gene="ybcF"
                     /locus_tag="ID725_RS08965"
                     /old_locus_tag="pgaptmp_001793"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGV7850412.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(24806..25621)
                     /gene="ylbF"
                     /locus_tag="ID725_RS08970"
                     /old_locus_tag="pgaptmp_001794"
     CDS             complement(24806..25621)
                     /gene="ylbF"
                     /locus_tag="ID725_RS08970"
                     /old_locus_tag="pgaptmp_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGV7850413.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(25632..26891)
                     /gene="ylbE"
                     /locus_tag="ID725_RS08975"
                     /old_locus_tag="pgaptmp_001795"
     CDS             complement(25632..26891)
                     /gene="ylbE"
                     /locus_tag="ID725_RS08975"
                     /old_locus_tag="pgaptmp_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGV7850414.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(26901..28568)
                     /gene="fdrA"
                     /locus_tag="ID725_RS08980"
                     /old_locus_tag="pgaptmp_001796"
     CDS             complement(26901..28568)
                     /gene="fdrA"
                     /locus_tag="ID725_RS08980"
                     /old_locus_tag="pgaptmp_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGV7850415.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            28885..29934
                     /gene="allD"
                     /locus_tag="ID725_RS08985"
                     /old_locus_tag="pgaptmp_001797"
     CDS             28885..29934
                     /gene="allD"
                     /locus_tag="ID725_RS08985"
                     /old_locus_tag="pgaptmp_001797"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGV7850416.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            29956..31191
                     /gene="allC"
                     /locus_tag="ID725_RS08990"
                     /old_locus_tag="pgaptmp_001798"
     CDS             29956..31191
                     /gene="allC"
                     /locus_tag="ID725_RS08990"
                     /old_locus_tag="pgaptmp_001798"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGV7850417.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            31202..31987
                     /gene="allE"
                     /locus_tag="ID725_RS08995"
                     /old_locus_tag="pgaptmp_001799"
     CDS             31202..31987
                     /gene="allE"
                     /locus_tag="ID725_RS08995"
                     /old_locus_tag="pgaptmp_001799"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGV7850418.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(32215..33360)
                     /gene="glxK"
                     /locus_tag="ID725_RS09000"
                     /old_locus_tag="pgaptmp_001800"
     CDS             complement(32215..33360)
                     /gene="glxK"
                     /locus_tag="ID725_RS09000"
                     /old_locus_tag="pgaptmp_001800"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HGV7850419.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(33382..34683)
                     /gene="ybbY"
                     /locus_tag="ID725_RS09005"
                     /old_locus_tag="pgaptmp_001801"
     CDS             complement(33382..34683)
                     /gene="ybbY"
                     /locus_tag="ID725_RS09005"
                     /old_locus_tag="pgaptmp_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HGV7850420.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTEVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(34740..36101)
                     /gene="allB"
                     /locus_tag="ID725_RS09010"
                     /old_locus_tag="pgaptmp_001802"
                     /pseudo
     CDS             complement(34740..36101)
                     /gene="allB"
                     /locus_tag="ID725_RS09010"
                     /old_locus_tag="pgaptmp_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(36138..37529)
                     /gene="ybbW"
                     /locus_tag="ID725_RS09015"
                     /old_locus_tag="pgaptmp_001803"
     CDS             complement(36138..37529)
                     /gene="ybbW"
                     /locus_tag="ID725_RS09015"
                     /old_locus_tag="pgaptmp_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HGV7850421.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(37698..38576)
                     /gene="glxR"
                     /locus_tag="ID725_RS09020"
                     /old_locus_tag="pgaptmp_001804"
     CDS             complement(37698..38576)
                     /gene="glxR"
                     /locus_tag="ID725_RS09020"
                     /old_locus_tag="pgaptmp_001804"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGV7850422.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(38677..39453)
                     /gene="hyi"
                     /locus_tag="ID725_RS09025"
                     /old_locus_tag="pgaptmp_001805"
     CDS             complement(38677..39453)
                     /gene="hyi"
                     /locus_tag="ID725_RS09025"
                     /old_locus_tag="pgaptmp_001805"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HGV7850423.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(39466..41247)
                     /gene="gcl"
                     /locus_tag="ID725_RS09030"
                     /old_locus_tag="pgaptmp_001806"
     CDS             complement(39466..41247)
                     /gene="gcl"
                     /locus_tag="ID725_RS09030"
                     /old_locus_tag="pgaptmp_001806"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGV7850424.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(41337..42152)
                     /gene="allR"
                     /locus_tag="ID725_RS09035"
                     /old_locus_tag="pgaptmp_001807"
     CDS             complement(41337..42152)
                     /gene="allR"
                     /locus_tag="ID725_RS09035"
                     /old_locus_tag="pgaptmp_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGV7850425.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(42230..42712)
                     /gene="allA"
                     /locus_tag="ID725_RS09040"
                     /old_locus_tag="pgaptmp_001808"
     CDS             complement(42230..42712)
                     /gene="allA"
                     /locus_tag="ID725_RS09040"
                     /old_locus_tag="pgaptmp_001808"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGV7850426.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            42942..43868
                     /gene="allS"
                     /locus_tag="ID725_RS09045"
                     /old_locus_tag="pgaptmp_001809"
     CDS             42942..43868
                     /gene="allS"
                     /locus_tag="ID725_RS09045"
                     /old_locus_tag="pgaptmp_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGV7850427.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            43937..45031
                     /gene="mnmH"
                     /locus_tag="ID725_RS09050"
                     /old_locus_tag="pgaptmp_001810"
     CDS             43937..45031
                     /gene="mnmH"
                     /locus_tag="ID725_RS09050"
                     /old_locus_tag="pgaptmp_001810"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGV7850428.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            45147..45512
                     /gene="ylbG"
                     /locus_tag="ID725_RS09055"
                     /old_locus_tag="pgaptmp_001811"
                     /pseudo
     CDS             45147..45512
                     /gene="ylbG"
                     /locus_tag="ID725_RS09055"
                     /old_locus_tag="pgaptmp_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(46262..46522)
                     /locus_tag="ID725_RS09060"
                     /old_locus_tag="pgaptmp_001812"
     CDS             complement(46262..46522)
                     /locus_tag="ID725_RS09060"
                     /old_locus_tag="pgaptmp_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850429.1"
                     /translation="MLAISSNLSKMIIFIIAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFPHVAVDISIYTNVEDDYFFLIFP"
     gene            complement(46503..>47678)
                     /locus_tag="ID725_RS09065"
                     /old_locus_tag="pgaptmp_001813"
     CDS             complement(46503..>47678)
                     /locus_tag="ID725_RS09065"
                     /old_locus_tag="pgaptmp_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGV7850430.1"
                     /translation="RRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALI
                     SEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGR
                     YITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTY
                     EDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRK
                     VKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKETIKALQDAGYLK"
BASE COUNT        12159 a        12416 c        11638 g        11465 t
ORIGIN      
        1 cttggagatg gaaaaataaa aaaataatag atatttcttg gtaaggaaaa tattccccga
       61 aagaggccac atggggaata ctgctcccct cactgatacg ggagtaccgt tacgacagcg
      121 cgggtaacgt atcaggcgtc accagccggg aagattacgg acgggaaaca cagcgggagt
      181 accggcttga ccggaacggc caggtcacgg cggtgacagc ctcaggcacc gggctgggct
      241 atggcgaagg cgatgagtcc tatggctatg acagctgtgg ctacctgaag gcgcagtctg
      301 cgggcgggca ccggataagt gaagagactg accagtatgc cggaggccac cggctgaaac
      361 aggccggaaa cacgcagtat gactatgacg ccgcaggccg gatggtcagc cgcaccagac
      421 accgtgacgg ctatcgcccg gaaacagagc ggttccggtg ggacagccgg gaccagctga
      481 ccgggtattg cagcgcacag ggtgagcagt gggaataccg ccacgacgcc agcggcagac
      541 gaacagagaa acgctgcgac cggaagaaaa tccgcttcac gtacctgtgg gacggcgaca
      601 gtattgcgga aatccgggaa taccgcgatg atgaactgta cagcgtacgg cacctggtgt
      661 ttaacggctt tgagctgata agccagcagt gcagccgggt acggcagccg cacccgtccg
      721 tggccccgca gtgggtgaca cgaacgaatc atgcggtgaa cgacctgacg ggccgtccgc
      781 tgatgctctt taacagtgaa ggtaaaaccg tctggcggcc gggacagacc agcctgtggg
      841 ggctggcact cagcctgccc gcagacacag actacccgga cccgcgcggg gagcgggacg
      901 cggaagccga ccccggcctg ctgtatgcag gacagtggca ggatgcagaa tcggggttgt
      961 gctataaccg gttccgatat tacgagccgg aaaccgggat gtacctggtg agtgatccac
     1021 tggggttgca gggaggggag cagacttacc ggtatgtgcc gaatccttgt gggtgggttg
     1081 atccgctggg attggctgca agttctaaaa tcagcagttt gatggactat attggcgatg
     1141 gtcgtcgtgt tagtgggcat acgggtttcc tggatggggt tcgtttatca cgtagtcaaa
     1201 taaacaatat tgctaaagaa atggagaagc taggaattaa agtaataagg aaagcagata
     1261 aatatttgcc accaaatgct agggcagctt ttgattatgg tcttcgcaat atttatctta
     1321 ggaaaaatgc taccttatat gaggtgtatc atgaagtgat tcatgctaag caatttgcga
     1381 aaattggacg agaagcatac gaagcactag gacgtttatc tagggaggaa cacgttctaa
     1441 atgaaatatt aaaaagtaaa aatttattca atgaagcgga aatagctcat gccataaaat
     1501 atgttgaggg attgagagaa aaattcatga tgggactaat aaattgataa aacataacaa
     1561 aataactatc gaaatggctc ttgacttggc tcggcgtgag ttagagttaa gggaaattcc
     1621 ttatattaaa aatagtctac atgccaatta cagctataaa tctattagta ttggttctaa
     1681 gcaaggttgg ttaatttcag cgaaattaaa agtccctgaa acttttgagc ctgatatgat
     1741 atttattgag atatctgacc cagaaggttt tattaatatt cctgatgtat tataaactac
     1801 gttagaggtt ttgttataag gtgttcggtt tctttacaga ccgctggcgt acatcgcaca
     1861 gaggccaggg aaagatcacc taacgccgct tttttgtact aaataattcg cactaaatgt
     1921 tcaataattg agatattcct tattatctaa aactgttttt cacagcttac atatgatcaa
     1981 atactcctta cataattaag gagaacaaaa tggaacttaa aaaattgatg gaacatattt
     2041 ctattatccc tgattacaga caagcctgga aagtggaaca taaattatcg gatattctac
     2101 tgttgactat ttgtgccgtc atttctggtg cagaaggctg ggaagatata gaggatttag
     2161 gggaaacaca tctcaatttt ttgaagcaat atggtgattt tgaaaatggt attcctgttc
     2221 acgataccat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac gagtgcttta
     2281 ttaactggat gcgtgactgc cattcttcag atgataaaga cgtcattgca attgatggaa
     2341 aaacgctccg gcactcttat gacaagagtc gccgcaggag agcgattcat gtcattagtg
     2401 cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag aaatctaatg
     2461 agattacagc tatcccagaa cttcttaaca tgctggatat taaaggaaaa atcatcacaa
     2521 ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag ggaggtgatt
     2581 atttattcgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag gaaaaatttc
     2641 cgctgaaaga attaaataat ccagagcatg acagttacgc aatgagtgaa aagagtcacg
     2701 gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt attgatttca
     2761 cgtttgaatg gaaagggctg aagaaattat gcgtggcagt ctcctttcgg tccataatag
     2821 cagaacaaaa gaaagagtca gaaatgacgg tcagatatta tatcagttct gctgatttaa
     2881 ccgcagagaa gttcgccaca gcaatccgaa accactggca cgtggagaat aagctgcact
     2941 ggcgtctgga cgtggtagtg aatgaagacg actgcaaaat aagaagagga aatgcagcag
     3001 aattattttc agggatacgg cacattgcta ttaatatttt gacgaatgat aaggtattca
     3061 aggcagggtt aagacgtaag atgcgaaaag cagccatgga cagaaactat ctggcgtcag
     3121 tctttgcggg gagcgggctt tcgtaatctt gccctgcctg ctcacctcca ggcacttcca
     3181 ctacatcatg acctgatcgg tgccaccact cctcttcact atgacgttaa cctcccctcc
     3241 ttgacaccac gcttttgtct acattctacg caaacggcaa cttttataac cagtgtaaaa
     3301 ataacgtgcc gcaatagtca tacctattaa tggtaaaaag ctgtcacaat tcataaaaaa
     3361 ccttaatata cgccacccta aacataacca gcgttaatgt aaggtttttg tgtggactgg
     3421 cttcttgatg tttttgctac ctggctctat ggcttaaaag taatcgcgat aacgttagcg
     3481 gtcatcatgt tcatcagcgg gctggacgat ttttttattg atgtcgtcta ctgggtacgc
     3541 cgcattaaac gcaagttgag tgtttatcgc cgctacccgc gaatgagtta ccgcgaactg
     3601 tataaaccag atgaaaaacc gttagcgatt atggttccgg cgtggaatga aacgggcgtc
     3661 atcggcaata tggccgagct ggcggcgacc acgctcgact acgaaaacta tcatatcttt
     3721 gttggcacct accccaacga cccggacact cagcgtgatg ttgacgaagt gtgcgctcgc
     3781 ttcccgaatg tgcataaggt agtctgcgcg cgtcctggcc ccaccagcaa agccgactgt
     3841 ctgaacaacg tgctggacgc catcacccag tttgagcgta gcgccaattt cgcttttgct
     3901 ggttttattc tgcatgacgc cgaagatgtg atttcaccga tggaattgcg tctgttcaac
     3961 tatctggtcg agcgtaaaga tctgattcag atcccggtgt atccgtttga acgcgaatgg
     4021 acgcacttca ccagcatgac ttacattgat gagttttcag agctgcatgg caaagatgtt
     4081 ccggtgcgtg aagccctcgc cggacaagtg cccagcgcag gcgtcggcac ctgtttcagc
     4141 cgccgcgccg tgaccgctct gttagctgac ggtgacggta ttgctttcga cgtgcagagt
     4201 ctgactgaag attacgacat tggcttccgc ctgaaagaaa aaggtatgac ggaaattttt
     4261 gtccgttttc cggtggtgga cgaagccaaa gaacgcgagc agcgtaaatt tttacagcac
     4321 gcacggacgt caaacatgat ctgcgtgcgc gaatatttcc ccgatacctt ttcgactgcg
     4381 gttcgacaaa aatctcgctg gatcatcggc attgttttcc agggctttaa aacccataaa
     4441 tggacctcca gcctgacgct gaactacttt ctctggcgcg accgcaaagg ggcaatcagt
     4501 aactttgtca gcttcctcgc aatgctggtg atgatccagc ttttgctgtt gctggcgtat
     4561 gaaagtttgt ggcccgatgc ctggcatttc ctttctattt ttagcggcag cgcatggtta
     4621 atgaccctgc tgtggctaaa ctttggcttg atggttaacc gcatcgtgca gcgggtgatt
     4681 ttcgtcactg gctactacgg cctgacgcag gggctactat ctgtcctgcg tcttttctgg
     4741 ggcaacctga ttaactttat ggccaactgg cgcgcgttaa aacaggtact tcaacacggc
     4801 gatccacgtc gcgtcgcgtg ggataaaaca acgcatgact tccccagcgt cactggcgat
     4861 acccgctcgt tgcgcccgtt aggtcaaatt ctgctggaaa atcaggtcat cactgaagaa
     4921 cagctcgata cagcactgcg taatcgcgtc gaaggtctac gcctgggcgg ttcaatgctg
     4981 atgcaggggc tgattagcgc cgagcagctg gcacaggcgc tggcagagca aaacggcgtg
     5041 gcgtgggaat ccatcgatgc ctggcagatc ccgtcctcgc tgattgccga aatgccggcc
     5101 tccgtggcgc tgcattatgc ggtactgccg ctgcgtctgg aaaatgatga gttaattgtc
     5161 ggcagtgaag atggtattga cccggtttcg ctggcggccc tgacgcgtaa agtcggacgc
     5221 aaagtgcgtt atgtcattgt tctgcgggga caaattgtca cggggttacg tcactggtat
     5281 gcacgccgac gcggtcacga tccgcgggca atgttgtaca atgcggttca gcatcagtgg
     5341 ctcacggaac agcaggccgg tgaaatctgg cggcaatatg tgccgcatca gttcctgttc
     5401 gccgaaatac tgaccacgct cggtcatatt aatcgttcag caattaacgt gttgttattg
     5461 cgccatgaac gcagttctct gccgctcggc aagtttttgg tcaccgaagg cgttatcagc
     5521 caggaaacgt tggatcgcgt cctgacaatt caacgcgaat tacaagtttc gatgcaatca
     5581 ctattactca aagcaggttt aaacacagaa caggttgcgc aactggagtc cgaaaatgaa
     5641 ggagaataac cttaatcgcg tcatcggatg gtctggttta ctgctgacgt ctttattgag
     5701 taccagcgca ctcgcagaca atatcggcac cagcgcagaa gagctggggc tgagcgatta
     5761 tcgccatttt gttatttatc cccgtctcga taaggcgctg aaggcacaga aaaataacga
     5821 cgaagcaacc gccatccgcg aatttgaata tatacaccag caggtgccgg ataatattcc
     5881 gctgacttta taccttgcgg aagcctatcg ccattttggt catgatgacc gggcacggct
     5941 gttgcttgag gatcaactga aacgtcaccc aggagatgcc cgacttgagc gcagtctggc
     6001 ggctattccg gttgaagtga aaagcattac aactgttgaa gaactgcttg cccaacaaaa
     6061 agcgtgcgat gctgcgccga ccctgcgttg tcgcagtgaa gtcgggcaga atgccctgcg
     6121 gctggcacag ttacctgtcg ccagagcgca actgaacgac gcgacgtttg ctgcatcgcc
     6181 ggaaggaaaa acgctgcgaa ccgatctgct gcaacgggca atctacctga aacaatggtc
     6241 ccaggcagat acgctataca atgaagcacg ccagcagaac acattaagcg cggcagaacg
     6301 ccgtcagtgg tttgacgtgc ttcttgccgg gcagctggac gatcggatcc tggcactgca
     6361 atcacagggg atcttcaccg atcctcagtc atatattact tacgcgaccg cgctggctta
     6421 tcgtggcgaa aaagcacgcc tccagcatta tctcattgaa aataagccgc tgtttaccac
     6481 ggacgcacaa gagaaaagtt ggctctatct gttatctaaa tacagcgcca accccgttca
     6541 ggcattggcg aattatacgg tgcagtttgc cgataaccgc cagtatgttg ttggcgcgac
     6601 gctaccggtg ctgttaaaag aaggtcagta cgacgcagcg caaaaactgc tcgccaccct
     6661 ccccgccaat gaaatgcttg aggagcgtta tgctgtcagc gtggcgaccc gtaacaaggc
     6721 tgaagctctg cgtctggcac gcttgctgta tcagcaagaa tcggcaaatc ttacccgcct
     6781 ggatcaacta acctggcaac tgatgcagaa cgagcagtca cgggaagctg ccgatttatt
     6841 gctgcaacgc tatcctttcc agggcgatgc gcgtatcagc cagactttaa tggcgcgact
     6901 ggcgtctctg ctggaaagtc atccttacct ggcaacgccg gcgaaggtgg cgattttatc
     6961 gaaaccctta ccgctggcgg agcaacgtca gtggcaaagt cagttgccgg gtattgcaga
     7021 taattgcccg gcaatagttc gcttgctggg cgatatgtcg ccttcctacg atgccgccgc
     7081 ctggaaccgt ctggcaaagt gttatcggga cacgctaccc ggtgtggcgt tgtatgcatg
     7141 gcttcaggcc gaacaacgac aaccgaacgc ctggcaacat cgtgcggtag cctatcaggc
     7201 gtatcaggtt gaggactacg ccaccgcact ggcggccagg cagaaaatca gtcttcacga
     7261 catgagcaat gaggatctgc ttgctgctgc caataccgcc caggcggcag gaaatggtgc
     7321 ggctcgcgat cgctggctac aacaggcaga acaacgtgga ctgggaagca atgccctcta
     7381 ctggtggctg catgcgcaac gttacattcc tggtcagccg gaactcgcac tgaacgatct
     7441 cacgcgctca atcaatattg cgccttctgc caacgcttac gttgcgcggg cgacaattta
     7501 tcgccaacgt cataatgtcc cggccgcggt gagtgatttg cgcgccgcgc tggaactgga
     7561 accgaataat agcaacaccc aggcagcgct tggttacgcc ttgtgggata gcggtgatat
     7621 cgcacagtcg cgggaaatgc tcgaacaggc gcataaaggg ctaccggacg atccggcact
     7681 gatccgacaa ctggcctatg tgaaccagcg tctggatgac atgcctgcga cgcagcacta
     7741 cgcccggctg gtgattgatg acattgataa tcaggcgctg ataaccccac tgaccccaga
     7801 gcaaaatcag caacgcttca atttccgccg tctgcatgag gaggtcggtc gccgctggac
     7861 gttcagtttc gattcttcca tcggcttgcg ttccggggca atgagtaccg ctaacaataa
     7921 tgttggcggc gcagcgccag ggaaaagcta tcgtagctac gggcaactgg aagccgaata
     7981 ccgcatcgga cgcaatatgc tgctggaagg cgacctgctc tcagtttata gccgcttctt
     8041 tgccgatacc ggagaaaacg gggtgatgat gccggtgaaa aatccgatgt ccggcaccgg
     8101 tctgcgctgg aagccgctgc gcgatcagat ctttttcctc gccgtcgaac agcagttgcc
     8161 gctgaacggg caaaatggcg catccgatac catgctgcgc gccagcgcct cattctttaa
     8221 tggcggcaaa tacagcgacg aatggcaccc gaacggttca ggctggtttg cccaaaacct
     8281 gtacctcgat gcggcgcaat atatccgcca ggatattcag gcgtggacgg cagattatcg
     8341 cgtcagctgg catcagaagg tagctaacgg acagactatt gagccttacg ctcacgttca
     8401 ggacaacggc tatcgtgata aaggcactca gggcgcgcag cttggcggtg tcggggtccg
     8461 ctggaatatc tggaccggcg agacgcacta cgacgcctgg ccgcacaaag tcagtctcgg
     8521 cgtcgagtat caacatacct ttaaggcgat taatcaacgt aacggagagc gcaacaacgc
     8581 gtttctcacc attggagtgc actggtaaat gcgtaagttc attttcgtat tgctgacact
     8641 gcttttggtc agcccttttt cctttgcgat gaaaggtatt atctggcaac cacaaaaccg
     8701 tgatagtcag gttaccgata cccagtggca ggggctgatg agtcagttac gtttgcaagg
     8761 cttcgatacc ctggttttgc aatggacccg ttacggcgat gcattcaccc agccagaaca
     8821 gcgcgcgtta ttgtttaagc gggccgcagc tgcgcaacag gctggcctga agctgattgt
     8881 cgggctgaac gccgatccgg aattttttat gcaccagaaa cagtcatccg cggcgctgga
     8941 aagctatctt aatcgcctgc tggctgccga tctccagcaa gccagattat ggagtgccgt
     9001 acctggcgta acgccggatg gctggtactt cagcgctgaa attgacgacc tgaactggcg
     9061 cagcgaagcc gcccgtcagc ctttgctaac atggttaaac aacgcgcagc ggctgattag
     9121 cgatgtttca gcaaaaccgg tttatatcag tagttttttc gccggaaaca tgtcgccaga
     9181 tggctatcgc caactgctgg aacacgttaa agcaaccggc gttaatgtct gggtacagga
     9241 tggcagcggc gtggataaac ttaccgctga acagcgtgaa cgttatctac aggccagcgc
     9301 cgattgccaa agctccgccc cagcaagcgg cattgtttat gaactgtttg tcgccggcaa
     9361 aggcaaaacc tttacagcga aaccgaaacc ggacgcagaa attgcctcgc tgttagcgaa
     9421 acgttcctct tgcggtaaag acactctcta tttctctctg cgctatttgc ccgtcgcgca
     9481 cggcattctc gagtattaaa tctcctccag gtaagtcggg tacgacctgg cttacctctt
     9541 tcgctctcgt cagaattcca tcaagattaa ttgctaaaaa gcgtgctaaa gaaagaagat
     9601 cgttatcggc gaacgggccg atcactcatg caggaagcat agatcctttg agcggagttt
     9661 ctgatatctg aggagtgcga aatgcaattg agcagcagtg aaccttgcgt ggtgatcctg
     9721 accgaaaaag aggtagaggt aagcgtcaat aaccatgcta cgtttaccct tccgaaaaac
     9781 tacctggccg ccttcgcgtg caacaataac gtcattgaac tctcaacgtt aaatcacgta
     9841 ttaatcaccc acatcaaccg taacatcatc aacgattatc tgttgttttt aaataagaac
     9901 ttaacctgtg taaagccctg gtcgcggctg gctaccccgg ttatcgcttg tcatagccgt
     9961 acaccggaag tgttccggct agccgccaac cacagcaagc agcaacccag caaaccctgc
    10021 gaagcggagt tgacgcgcgc attgcttttt accgtattgt ctaactttct tgagcaatcg
    10081 cggtttattg ccctactgat gtatatctta cgcagcagcg tccgcgacag cgtctgccgc
    10141 attattcaaa gcgatattca gcattactgg aatctgcgaa ttgtcgccag ttcgctatgt
    10201 ttaagcccca gcctgctcaa aaagaaatta aaaaacgaaa ataccagcta tagccagatt
    10261 gtcacagagt gtcgtatgcg ttacgccgta cagatgttat tgatggataa caaaaatatc
    10321 actcaggtgg cgcaattatg tggctatagc agcacgtcgt actttatctc tgtttttaag
    10381 gcgttttacg gcctgacacc gttgaattat ctcgccaaac agcgacaaaa agtgatgtgg
    10441 tgaagggcaa agcggaaacg gataagacgg gcataaataa agaagaaatg gcgcgccctg
    10501 caggattcga acctgcggcc cacgacttag aaggtcgttg ctctatccaa ctgagctaag
    10561 ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac ccttgctgag
    10621 tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt ttctgattta
    10681 tttcgctata gcggcaaaca aacgcacacc gctgcgcgtc tgaatcaaga aaacccgtat
    10741 tttcatgtat caaagtacaa tttaccgccc taacggaaaa ttgtccgctc ctatgagact
    10801 ggtaactatg aaaccaacgt cggtgatcat tatggacact catcctatca tcagaatgtc
    10861 tattgaagtt ctgttgcaaa aaaacagtga attgcagatt gtcctgaaaa cggatgatta
    10921 ccgcataacc atcgattatc tccgaacccg tcctgttgat ttaatcatta tggatataga
    10981 cttgcccgga acagacggtt ttaccttcct gaaaaggatc aaacaaatcc agagcacagt
    11041 gaaagtgtta tttttatcat cgaaatcaga atgcttttat gctggcagag cgatacaagc
    11101 tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat gatatttttc atgccgttca
    11161 gatgatcctc tccggataca cgttttttcc cagcgaaacg cttaactata taaaaagcaa
    11221 taaatgtagt acgaatagtt caacggtcac tgtgctatct aatcgtgaag tgaccatatt
    11281 acgttatcta gttagcggat tatctaataa agaaattgcc gataagttat tacttagcaa
    11341 taaaacagtt agtgcgcata aatctaatat ttatggcaag ctaggtttgc attcaattgt
    11401 agagcttatc gactacgcca aattatacga attaatataa tattaattat aattgatcat
    11461 aaatatcgca tccgctttcg ccacacctgg ccgaacaccg ctggctaacg ctcgataatt
    11521 ggcaaaaaag tttagtgtga cattgccatt tgcatctact tcctcagtcg ggctcgcctc
    11581 ccccagtgcg agccgggagc gatcgctatt acgtaattcg atggcgacgg tttgtgccat
    11641 tgcgggatca tccagagcca gcaggttggc atcagatgcc ggcgttcccg taaataaaat
    11701 cgcaactgaa cccggaggac atccctccag ccgcaggcta aaagggagga gtgccgtggt
    11761 atcgccagcg ttcagtaatt gtgtcgtagg ccatctgcct aaatctaccg tcttatcaat
    11821 atccgctgtg tttacggtac aggagaaatc aacaacgtta ccgtgcaaat tgatattaat
    11881 cgttcctaaa gggtcagctg cccatccact ggaactccac agtaacccgc agaaacagct
    11941 aaagaatact cttctcattc tccgtacctc attgataatc gacgcgtaaa taccccagcg
    12001 cgctaaacgg cccttcggtc ggtttttgac cggtaatact gataggccag gcgcgaagtg
    12061 tgacattggc tgccgcagct gcatccagac ggaaaggaat aacgctattg agatcgttag
    12121 gcgtgatcgg cgtatcgttc tgatcggcga caataaaacc taaatcctga ttgtccgaca
    12181 ccatcgcctg accagaaacg gcactggctt ccagacgcat tgttaaataa gcctgcgcag
    12241 caacattcgt acatttgacc gcaatgctct tggtttgcgg catgacacca gcaggccgat
    12301 tacccggccc tgccgcacta aataacgatg cgccgatatc accaaaatca aattcaacaa
    12361 tctgcccggc atttaattcg cagttttgcg gtacttcaac ccggccacca aaactaatgg
    12421 tataaacagg tgtaaccaac ggatcgtaag gcgttgatgt cacatacacg gtaaacatca
    12481 cctgtctggg gatctccacc atgttaataa agggccgaat aacctttaaa cgaaatatca
    12541 aacgagaatc catcaccccg aacggaaggt tttgcgaaac gttaggatct gttcccatac
    12601 gaatataatt ttgcggggga aattgaatat ccatcacact atcaaccaga ctcatcgcac
    12661 ctaatagata atcatttaat tgcatatatt tataattatc gatagtttcc tgaacaataa
    12721 agttagtgac ataactacgg tatgtataat tcaccagcgt gccgggtgga caaattgctg
    12781 agacacctac ccagccggat ttttccggta aaacaacaat ctgccctggc tggttattgg
    12841 tgctattaaa gacatttgag agatcatagt gttcatcttc tgctacgcca gtactatttg
    12901 cacaaacagt agcccaggat atgtttgcca gacacaaaca tgccatcgcc aataataatc
    12961 tgcagattat cttcattgat aatttccttt atgagccagg atgtgtgcaa actgccgaaa
    13021 taacagtgac ggcttgctgt aagctttgct tcggcaatac atagtgcgca atgcagcgtg
    13081 agttcgcgcc gtctccccac tgaaccagta attcaccaga caatggcgca ccacttaaat
    13141 aaacttgccc gtcatcaccc accatactgg ttattccggt actgttttcc cgtaccagtg
    13201 atccaaacgg caccggtttt ccgccctggg taacggtaat gagcgcccgc acgcctatgc
    13261 gggtatcaaa attggcacga accaacgcgc cttgcgtcgg cacaacgctg ctaatatttt
    13321 tttcaacatc gatggaattc cccatcgtat tggtatcaag cgcgatacgg ttataccgat
    13381 aaaccgtggc atacggcatc accgcatagc cgcgccagtc ggttaaaatg ccagtttgat
    13441 tttcaatgcg tacaccgcct gcgccaggcg ctttaatcaa aacattggta tcccctaaag
    13501 gctggctcag cgttatgcca ttttcatgtc cgaccacacc gccagacagc tgccagttaa
    13561 cgtcatgttg atcgcggtcg tagttatacc cgacccccag cgtaccgtaa gcggcctgcc
    13621 agtttgccgt ggcgctgccc gtatacccat tattcgaggt atcaccctgg cttacgtgat
    13681 aactcaggtt gtggccttcc agtaaggtcc cacctacacc tgccagccag ctattttgcc
    13741 cgttgctgtt acggttggcg ttaaaggagg cataagcgcg gtcgagcgca ttttcccggg
    13801 tgtagcgacg tttggtcaga acattgaaag gcactgaaac attaagtccg acaatacgtt
    13861 cgttatcggg gatccctaca gattcattcc acgaaaatga gagtgaataa ctgatgccaa
    13921 cccagctgct ggtatacccc acctgatacc aggtatctga atccgaagta ttccagtatt
    13981 tttgatgggt accagagata taaagcgagc caaagtcatt aagtgattgt gaaatattta
    14041 actgcaaacg gtctttacgg ctaaagcgta aattgtggta attcacgatt atcggttcat
    14101 cccgatgctc accgtcataa tcgtaatcat attcgtaccc ctccattcga cgatacgcaa
    14161 catcatcaag ggtataaaaa ccttgtgtcg aatagcggta acccattaac tgaaaattgg
    14221 tgccgaaggt gttcatcgat ttcgcataga ggaagcgaat ggaatccccc tcatgacgac
    14281 tgtcgtcggc taactgactg cgcgcatggg tcacatccag cgacactgcg ccccagttcc
    14341 cgaggttgcg ccccagccct aataaaaagg cggtgtaatt ggcagataat tgcgtcccgc
    14401 cgtaggcagt aaattcctgt ggtaaaccgc cgagtgccgt gccctgaaaa aagaaaggcg
    14461 atgattgctg actattaccg ctacgaaaat cgcccgccgt caggtcaaat ttgaaacgcc
    14521 cttcgcgttg taaaattggc actgttgaat acggaatagt gtaattctgc tgattgccat
    14581 cgcgttcgtc gatggtgacg tccagatcgc catttgaaga tgtcgggtgc aaatctgtaa
    14641 tttcaaaagc gccgggggaa acatagcttt gatagataat aaaaccattt tgccgaatcg
    14701 ttagctgggc cgccgtacgg gcaatcccac gtaccgttgg ggcaaaccct tgctggctat
    14761 caggatacat attatcggaa gaataaagcc gtacaccacg aaatccaaca ccatcgaaaa
    14821 tatcacttcc tgtattgccg tctcccatta ccagttcact tttcagcgga ataatggcgc
    14881 gctgtaccca ggtgccaata ttattccact gttctgaatg atatccattt ccgcgaaaat
    14941 agttccagga accattgttg cgtaaacgcc acgggccaat attaatcccg ctgagctcac
    15001 taaaaaaata gctatcgtta ccattacctc tgttaccggt gaaattataa ttcaggagta
    15061 aagcaggaat accttcatcc cactcttcag gcggaatgta accgtgagcg ctactcaaca
    15121 acgctatctg aggaatagtg atgttcaggc gcatcgctgc aaaatcaaag ttaattgtgg
    15181 catcaggtac agctttcagt aaattgatgc atttattgtt ttgctgctgg gctaactcgg
    15241 gaaatgcact gctattaagg ccaattcgtt caaggagtac ctggttaaaa cagggcatta
    15301 acccacctga tttatcacct gtattttctg ttgtcgattc aaatacgata tcctgtgaac
    15361 caatgaactc atcattacgc cagagatcca cccgatacac gcccgcaggt tgatgattac
    15421 ctctctcaaa gcgcgataaa tcagcaacga atgtgccatt ctctaataaa aatgccgggt
    15481 tgaaataact ttccgcatag cttaaaaagg agggtaaaat ggcaagagat gaataacaaa
    15541 ttccggccag acaccaggta tacctctgcg gaatatccgt agtagtgggt attttcataa
    15601 ttagctctga tattgtcaga tgattcggtt agtttaagtt cacttctctg accggggtta
    15661 ctgaaccata atcattaacg gtctgaaatt tcagctttcc ctgcactcct gagggtaagg
    15721 gaatttgtga attaactctg ggtgcagcca tagcattccc caacttttgg ctgcccactt
    15781 gtaagttaac cagggtgaca taaaaaggtg atggattatg aacattgata tagttaccgg
    15841 atcgcgaaaa cttgagtgta tccggcgctt ctgcgggtaa ttcttgtagt tgaattggac
    15901 ggagaaataa cttcatgcgc gataaaatcg ccagttgcaa aacattcctg ccgttcaatg
    15961 catttttatc taccgaagga atcgtcttaa cattcatcca gaacagagac tcacgatctg
    16021 ctgccagcgg tggaccggtg taaataatac gcaaagtatt ttcgcttttg ggttcactaa
    16081 caaatagtgg cggtgtaatg atgaatgact tttcttttac accgctgctg ttttcaatcc
    16141 acgaattgac cagaaaacgc tcattggtat ggctatttct aatccatacc gcagtctgtt
    16201 tagcatcagc gggataaata atacgcgtgg cacctaatgc gatccctccg gcagcatgga
    16261 cgctggccga aatgattaaa taaaatataa tgagcatcaa taactttatt ttagtcatca
    16321 tagttaatac gccttgtgta ttcctgacgg caaaaataag aaatataaag aaatgattgc
    16381 tttggtttct tacaacacat cattcccttt tcatttaata aagcggcatg atacaaagtg
    16441 aacatgattt cgatttacat tgccaccata ccgcttcaga caaccggtac attactttgt
    16501 tataagacgt gatatataat tcaaaacaac gtggttttga ttattcgtat ctcataataa
    16561 aagtcgcgtc agcatttgct tgccctgctg atgcacttgt accggtgcct ttataacgtg
    16621 cagaaaaatg aagaacgttg gtcccgggga tcaagttctg gttggttgag aagctattcc
    16681 catcaggttt gagaattgcg gatgtattat caagtatttc aataccgaca cccgttgctg
    16741 ttgttgtatt ggtactggat gcaatggcca ataaattatc attgattgca tcagcctgac
    16801 cagaaaatgc aacggcagca ttagcggcaa caacaggatc gcagtcgttc aactgaatgg
    16861 tgaatggaat taatgcagag gtattaccaa cagcattgaa aatatcggta cgatattgac
    16921 cgagtgtgac aacctggtct gccgagtcag tattcactga acaggcagca ttcaccagtt
    16981 caccttcaaa gtgaattgta ccgccatcta caacggcagc ataactaccc gtagcggcga
    17041 atagtgcggc agccagcgca gacgaaataa atcttaattt catatatatt ccttcaatct
    17101 catttatcga ctccacatcc gtatataacc gattacttta tttaagacac tgatagtagt
    17161 aaattccttt ttatcctcta agaatgtctt aattgaaaat atgcactcta ttctaaaaaa
    17221 tagagagccc cgttagatga atacttccgc gcaaaatata ttcaacacaa atatagacct
    17281 gaagcggtaa attaccaggc tgaaaattct ttttatattg tcaggtattt cttaaattat
    17341 cttaatcctt aaacaaggaa ataaatcagt tccagattta caacgccatc atggacgaaa
    17401 aatgaagctt tcaatctcag cgacctcagc gacagtgcgc ctaaccttcg caagaggtcg
    17461 cttcacgcga taaatctgaa acgaaacctg acagcgcgcc ccgcttctga caaaataggc
    17521 gcatcccctt cgacctacgt aacagatgga atcctctctc tgatggcagc aaagattatt
    17581 gacggtaaaa cgattgcgca gcaggtgcgc tctgaagttg ctcaaaaagt tcaggcgcgt
    17641 attgcagccg gactgcgggc accaggactg gccgttgtgc tggtgggtag taaccctgca
    17701 tcgcaaattt atgtcgcaag caaacgtaag gcttgtgaag aagtcgggtt cgtctcccgc
    17761 tcttatgacc tcccggaaac caccagcgaa gcggagctgc tggagcttat cgatgcgctg
    17821 aatgccgaca acaccatcga tggcattctg gttcaactgc cgttaccggc gggtattgat
    17881 aacgtcaaag tgctggaacg tatacacccg gacaaagacg tagacggttt ccatccttat
    17941 aacgtcggtc gtctgtgcca gcgcgcgccg cgtctgcgtc cctgcacccc gcgcggtatt
    18001 gtcacgctgc ttgagcgtta caacattgat accttcggcc tcaacgccgt ggtgattggc
    18061 gcatcgaata tcgttggccg cccgatgagc atggaactgt tgctcgcagg ttgcaccacc
    18121 accgtgactc accgtttcac taaaaatctg cgtcaccacg tagaaaatgc cgatctgttg
    18181 atcgttgccg tcggcaagcc gggctttatt cctggtgact ggatcaaaga aggcgcaatt
    18241 gtgattgatg tcggcatcaa ccgtctggaa aatggcaaag ttgtgggcga cgtcgtgttt
    18301 gaagacgcgg ctaaacgcgc ctcatacatt acgcctgttc cgggcggcgt tggcccgatg
    18361 acggttgcca cgctgattga aaacacgcta caggcgtgcg ttgaatatca tgatccacag
    18421 ggtgagtaac atggcgacat tttctttagg taaacatccg cacgttgagc tgtgcgactt
    18481 gctgaaactg gaaggctgga gcgaaagcgg cgcgcaggcg aaaatcgcga ttgccgaagg
    18541 ccaggtgaaa gtcgacggcg cggttgaaac gcgcaaacgc tgcaaaatcg tcgccgggca
    18601 gacagtgagt tttgcaggtc acagcgtaca ggttgttgcc tgatcccttt cccggcctgt
    18661 atcaacaggc cggttctctt ttcagacttt tgccctctac gccccgttca tccatgctga
    18721 tagcatcgac attttgccga agcgaccatc atgccaaccg ttattacgca cgccgccgtt
    18781 cccctatgca ttggtttagg gttgggttcg aaagtcatcc ctccgcgcct gttatttgcc
    18841 ggaatcatcc tggcgatgct gcccgatgcc gacgtcttgt cgtttaattt tggcgttgct
    18901 tacggcaatg tttttggtca tcgcgggttt acccattcgc tggtgtttgc gtttgttgtc
    18961 ccgctgttat gtgtgctgat tggacgtcga tggttcaggg ctgggctgat tcgctgctgg
    19021 ctgtttttaa ccgtctcgtt gctatcgcac agcttgctgg attcggtaac cactggcggt
    19081 aaaggggttg gctggctgtg gccgtggtca gatgaacgct ttttcgctcc ctggcaggtg
    19141 attaaagtcg cgccgtttgc attgtctcgt tacaccacag cgtacgggca tcaggtgatt
    19201 atttctgaat tgatgtgggt gtggttgccg gggatgctac tgatgggaat gttgtggtgg
    19261 cgcagacgat aaccggatgc gaaaactcgc atccggcaat agcgcaatta cttacgacgc
    19321 caggtggtcc cttgcgggcc atcttccagc acgatcccca tctcattaag acggtcacgc
    19381 gccgcatctg ccgccgccca gtctttcgct ttacgggcat ccagacgctg ttgaattaac
    19441 gcttcaatct cagccacttc gctgtcgtct gcctgcgcgc cgctttgcag gaacgcttcc
    19501 ggttcttgct ccagcaggcc caatacggcg gaaagtttac gcaggtgaga tgccattgca
    19561 ttcgccgctg ccatatcttc tactttcaga cggtttactt cacgcgccat atcaaacagc
    19621 acggaatagg cttccggggt gttgaaatcg tcgtccatcg cttcaataaa gcgcgcttca
    19681 aacgcttcgc caccggcagg cgcaacggtt ttatctgtgc cgcgcagcgc agtgtagaga
    19741 cgctccagcg ccgcacgcgc ctgcttcagg ttctcttcgc tatagttcag ctggctgcga
    19801 tagtggcccg acatcaggaa gtaacgcacg gtttccgcgt cgtagtattt cagcacatcg
    19861 cgcacggtaa agaagttacc cagcgatttg gacatcttct cgcggtcaac catcaccatc
    19921 cccgagtgca tccagtagtt cacatactga ccatcatggg cacaggtgga ctgcgcgatt
    19981 tcgttttcgt ggtgcgggaa catcaggtct gaaccgccgc cgtggatatc aaagtggtta
    20041 cccagctgct tgcagttcat tgccgaacat tcaatgtgcc agccaggacg acccgcgccc
    20101 cacggagacg gccagctcgg ttcgccctct ttcgacatct tccacagaac gaagtccatt
    20161 gggttgcgtt tgtcgtcgac cacgtcaacg cgcgcgcctg cctgcagctg gtcgagatcc
    20221 tgacgcgaca gcacgccata agttggatcg gtcgggacgt cgaacatcac gtcgccgttg
    20281 tccgccacat aagcgtgacc tttggcgatc agttgttcag tgagttcaat aatttctgcg
    20341 atatggtgcg tcgcgcgcgg ctccatatcc gggcgcagaa tgttcaacgc atcaaaatct
    20401 ttgtgcattt cggcgatcat gcgatccacc agcgccacaa agctttcgcc attttcattg
    20461 gcgcgtttga tgattttgtc gtcgatatcg gtaatgttgc gcacatactt cagcttatag
    20521 ccgaggaaac gcagatagcg cgcaaccacg tcaaaagcaa caaaggtacg cccgtgaccg
    20581 atatgacaga gatcgtaaac ggtgattcca cacacgtaca tgccgacttc cccggcgtga
    20641 ataggcttaa attcctcttt ttggcgtgtc agagtattga agatttttag catcgaagat
    20701 tccgtttaga catgtgtggg taattgagtt gcgtataata cccatatttc ccgcgcgaat
    20761 cagcatacat tgcgtgatga tccgatcgtg cggttatgct ataacaccac cctatatatg
    20821 acccgaactg ggttgaagca ccaatcaaac ggaacaggat gcaaaaatgg ttactttcca
    20881 caccaatcac ggcgatattg tcatcaaaac ttttgacgat aaagcacctg aaacagttaa
    20941 aaacttcctg gactactgcc gcgaaggttt ttacaacaac accattttcc accgtgttat
    21001 caacggcttt atgattcagg gcggcggttt tgaaccgggc atgaaacaaa aagccaccaa
    21061 agaaccgatc aaaaacgaag ccaacaacgg cctgaaaaat acccgtggta cgctggcaat
    21121 ggcccgtact caggctccgc actctgcaac tgcacagttc ttcatcaacg tggttgataa
    21181 cgacttcctg aacttctctg gcgaaagcct gcaaggttgg ggctactgcg tgtttgctga
    21241 agtggttgaa ggcatggacg tggtagacaa aatcaaaggt gttgcaaccg gtcgtagcgg
    21301 tatgcaccag gacgtgccaa aagaagacgt tatcattgaa agcgtgaccg ttagcgagta
    21361 atcgtggcga cactctttat tgcagatctt catctctgcg tggaagaacc ggcgatcacc
    21421 gccggttttc tgcgtttttt agcgggagaa gcccgcaagg ccgacgcgct gtatattctt
    21481 ggcgatctgt ttgaagcatg gattggcgac gacgatccca acccactcca tcgccagatg
    21541 gcggcagcga tcaaagcggt gtccgattcc ggcgttccct gttatttcat tcatggcaac
    21601 cgtgattttc tgctcggcaa acgctttgcc cgtgaaagtg gcatgacgtt attgccggaa
    21661 gaaaaagtgc tcgaacttta tggtcgtcgg gtgctgatta tgcatggcga cacgctgtgc
    21721 accgatgacg cgggttatca ggcttttcgc accaaagtcc acaaaccctg gctgcaaacg
    21781 ctattcctcg ctctgccgtt gtttgtgcgc aaacgcattg ccgtgcgaat gcgcgcgaac
    21841 agtaaagaag ccaacagcag taaatcgctg gcgatcatgg acgttaacca aaacgcggta
    21901 gtcagtgcga tggaaaaaca tcaggtgcaa tggctgatcc acgggcatac ccatcgcccg
    21961 gcggtgcatg aacttatcgc caatcagcaa cctgcttttc gcgtggtact gggtgcctgg
    22021 catacggaag gttcgatggt gaaagtcacg gcggatgacg ttgagctgat tcattttccg
    22081 ttttaaaaaa cccgcaactt tgctgatttc acagccacgc aaccgttttc cttgctctct
    22141 ttccgtgcta ttctctgtgc cctctaaagc cgagagttgt gcaccacagg agttttaaga
    22201 cgcatgtctt cccgcaataa tccggcgcgt gtcgccatcg tgatggggtc caaaagcgac
    22261 tgggctacca tgcagttcgc cgccgaaatc ttcgaaatcc tgaatgtccc gcaccacgtt
    22321 gaagtggttt ctgctcaccg cacccccgat aaactattca gcttcgccga aagtgccgaa
    22381 gagaacggtt atcaggtgat tattgcgggc gcaggcggcg cagcgcacct gccaggcatg
    22441 attgccgcca aaacgctggt gccagtgctg ggcgtgccag tacagagcgc cgcactgagc
    22501 ggtgtcgata gcctctactc catcgtacaa atgccacgcg gcattccggt gggtacgctg
    22561 gcgattggta aagctggcgc ggcaaacgcg gcattactgg cagcacaaat tcttgcgact
    22621 catgataaag aactgcacca gcgtctgaat gactggcgca aagcacaaac tgatgaagtg
    22681 ctggaaaacc cggacccgcg aggtgcggca tgaaacaggt ttgcgtcctc ggtaacgggc
    22741 agttaggccg tatgctgcgt caggcaggcg aaccgttagg cattgctgtc tggccggtcg
    22801 ggctggacgc tgaaccggcg gcggtgcctt ttcaacaaag cgtgattacc gctgagatcg
    22861 aacgctggcc ggaaacagca ttaacccgcg agctggcgcg ccatccggcc tttgtgaacc
    22921 gcgatgtgtt cccgattatt gctgaccgtc tgactcagaa gcagcttttc gataagctcc
    22981 acctgccgac tgcaccgtgg cagttacttg ccgaacgcag cgagtggcct gcggtgtttg
    23041 agcgtttagg tgagctggcg attgttaagc gtcgcactgg tggctatgac ggacgcggtc
    23101 aatggcgttt acgcgccgat gaaaccgagc agttaccggc tgaatgctac ggcgaatgta
    23161 ttgtcgagca gggcataaac ttctctggtg aagtgtcgct ggttggcgcg cgctgctttg
    23221 atggcagcac cgtgttttat ccgctgacgc ataacctgca tcaggacggt attttgcgca
    23281 ccagcgtcgc ttttccgcag gccaatgcgc agcagcaagc gcaagccgaa gagatgctgt
    23341 cggcgattat gcaggagctg ggctatgtgg gcgtgatggc gatggagtgt tttgtcaccc
    23401 cgcaaggtct gctgatcaac gaactggcac cgcgtgtgca taacagcggt cactggacac
    23461 aaaacggtgc cagcatcagc cagtttgagc tacatctgcg ggcgattacc gatctgccgt
    23521 taccgcaacc agtggtgaat aatccgtcgg tgatgatcaa tctgattggt agcgatgtga
    23581 attatgactg gctgaaactg ccgctggtgc atctgcactg gtacgacaaa gaagtccgtc
    23641 cgggccgtaa agtggggcat ctgaatttga ccgacagcga cacatcgcgt ctgtccgcga
    23701 cgctggaagc cttgatcccg ctgctgccgc cggaatatgc cagcggcgtg atgtgggcgc
    23761 agagtaagtt ctgttaacct ggtgctcatt tgattgccgg atgcggcgtg aacgccttat
    23821 ccggcctaca ccgatccggt acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc
    23881 aggcatggtg cgccgattgc cggatgctct catcactaca gcgaaataca ggtccccgct
    23941 tcgcccgcca gcgtctcttc aattcgcgat aacgccccaa tccacgcggg tttaccacgg
    24001 cttctgacat aaccactcac cgccgttacc ttcggcccca tcgaaccatc ggctttggca
    24061 aatggcgcta actcatccgg tgtggcatgg cgaatggcac gttgctgcgg cattccccag
    24121 ttttcatata ccgcatcagc atcggtgagg atcaccagtc catcggcgtt aatttgctcg
    24181 gcgagcaacg cagcggcgag atctttatca atcactgctt cactccctgc tccgtcatcc
    24241 gtcacaggca caccgccgcc gccactgcaa atcaccacat gcccctcttt gagcaacaac
    24301 tcgatggctt cgctgtcgag aattttacgc ggttgcggag acgccaccac ccggcgcaaa
    24361 tatttaccat cacgtttcat ctgccagccg taagccgctt ccagtgcctc ttgttcttct
    24421 ggctgataaa ccggaccaat aaatttctct ggctgcaaaa acgccggatc atcaggcgaa
    24481 acttcaatgc gcgtccgcac cgtcgtgaca ggcggcatct gcggctgtgc gctcaaactc
    24541 tgcgccagca tatagccaat catcccctga ctttctgcca ccagcacatc cagcggatac
    24601 ggttctacct ctttccacgc cagattctgc aatgccagca gccccacctg cggcccgttg
    24661 ccgtgaacaa tcgccagccg ataagaacgg gccaggcgcg ccagcgcggg tacagcactg
    24721 gcgatattgc gatattgatt ttctgccgtc agcgcctcac cgcgctggag taaggcgttg
    24781 cccccaagag ctacaaccag tgttttcatg gttttccttg taataattgt tggccaagcc
    24841 agaaccccag cagtgtatct gcccctgacg tatgccccag cgccagcagc gaatcaatcg
    24901 caacagcggt acgtttcggg caactcagag catgaacaaa gtgcaggagt ggcgaggcga
    24961 aatatccttg cgcggcataa cgtaaataac tgacgctcac cgctgtggta acgagttgca
    25021 gattgtcgga acaggcaaaa aacggacgac cggagcgcgc atctaaagca ccataatacc
    25081 aggccgccag cagcattccg ctcagcgtgt catcatgact tggcgttaat ccggggcctt
    25141 tacccagcca gtgccgccag tcggtcttaa cgccattgag cgcggcctga aaacagtgac
    25201 gaaactggcg taactcagca ggcagcggat cgcttgccgc caacgccagt ggcccgaaaa
    25261 gcccggtttc ctccgcgcgt tgcatccatg cagctgcaag tggttgagga tgcgcaggcg
    25321 gcgtaatacg cagcaaacaa taacgctgtg gctgtttaac cgtgaaacgc ccgaggcgaa
    25381 tcccttgagc cacaacctgt ggtcgttcat tgccgcataa tccaccgcat aacgcatcga
    25441 attgcgcacg gcgaagcacc catcctccgg ggccgaaacc actaccctga cgatgcagcg
    25501 ttaacagttc gccgctttcc gtcatcaggt taatcgcccg ccgccacact cccgctaacc
    25561 gccaggactg gcgataattc ggtgcgctac tactggcaag cagaggatgg atgatcgtca
    25621 ttacgcgttc ctcaaccaat ccccatgctt tcagccagtg cttccagcgc ctgttcaaag
    25681 cacgccagcg gtgcccgcac ggtgcctgcg ccaatctgcc cgatccccgc ctctttatgg
    25741 gcgataccgg tattgatgag tggcgtaatg ccggtttcta ccacgcgacg aatgtccagt
    25801 cccaggcacg cgccctgaaa atcccagctt gggatctgca actgcatatt gcgttcaagg
    25861 taaatttccg ccatctcttc agataccgcc ctggccgctt ccatgccact cgcaccgaca
    25921 aagcgcgtta cgccaggcgc tgcgatcatt gccgcacctc cgataccaaa ggtttcggta
    25981 atcgcgctat cgcccatatc cgggttcgcc tgctcctgcg agaagccggt gaaaaacaga
    26041 ccttgcggag tgtttacagg cgcagtaaac cagcgttcgc ccagcccgct tacccgaatc
    26101 ccgaacatat tgccgttgcg ggtcattgcc gtgacgatgc tgcctgcgcg gatcatcgcg
    26161 ccagcatcca tcgccgcctt gcagtaagcc atcgcgaggt tgaggaagaa ctgatcggtc
    26221 acgctgagga aatccatcac ttccgcgatg tgctgtttat catgatcgag gcgagcaatt
    26281 tgtggggcca gcgcacgcat taacagtgct gaggaagcaa tattgcgttg atggaactca
    26341 tcgcccatcg taatgccctg cgccatcatc gcagtaagat cgataccgcg ctccatgcgc
    26401 cccagcgccg cgcttaatac tggcattaac acatcgcgca tccagcgatg gcgagtcagg
    26461 acatcttcgc cgtaagcgcc aaaacgcatc actttgccga taccttcgtt gaggttgcag
    26521 tacgcccggt tgccgtcggt cacgttctcg accaccagca tcggcatact ggcagaagta
    26581 ataccgccca ttggcccgac ggcattcaca tggtgacaag gaatgaagtt cacttccccc
    26641 tgctccagta ttgccagcgc ctgcgcttca tctttcgccc aaccttcgaa cagacatgcg
    26701 cccacgcacg cccctttcat gggtccggtc atctcctgcc agcgcattgg cggcccggcg
    26761 tgaagcagtg ttttgccctc gtttagttcg ctgataagtg aagaagccgg ttgcacatcc
    26821 agccagtgtg gacgagcgcg acgaatttgt tcgataaccg cagcattggc ttgcgccact
    26881 gatgtaaaca tgagaacccc ttattgcaaa cgttctaata aacgagccag ttttttattg
    26941 ccgccggcga ctggcgacca ttggtaatgc acaaccggtt tgctggcgct ttgtagctcc
    27001 agcgcaaaac tgcgtaatcc gatgttaatc acggcgacgt tttccagtaa tgacggtgtg
    27061 tgttgctgtg cggcaggcga gagcggatga attaacgctg ccgccagcaa ggtggcttcc
    27121 ggtagcgaac tcacgaccgc aatccccgca tcttccagcg tggcgatttg ctgcgagcgg
    27181 cattgcgggt cacgttcagt gcctgtcacc gtggcaatgg catacagtgg ttgattgtct
    27241 gaacgcgcag cacaggcttt ttgccaggcg cttaccagcg aggcggcagg atcggcggtc
    27301 gcaccgaagc caatcacgac atcaagcagc agcacacgca cttgcggttt agcgccgaga
    27361 tcggtaatta actggttgcg taaggttggg tcgatcatcg gatggggacg cccgacggtg
    27421 tagaaatcat cgccgaggtc aataatctgg tggccatcgg cgtccagcat catgccgtga
    27481 tgatgggcat catcggcttc cacgccaagg tgtccggcaa gtaatcccgc cgcttcggca
    27541 gccagcgtac cgccggtata caaaccgcaa ataaatccgc tgctggcagg cattatcgcg
    27601 ttacgtcgcg ccgtaacgcg tgaaagcagg caagccaggc gtgcggcctc atccagcgag
    27661 gaggcaaacc agacattctc gtcgcgggcc accgccgggg tataacctaa aaacagcgcc
    27721 accgtcggtt tgccggttgc tttcatggca ttaacaattt tcagacgcac cgcttcggca
    27781 ggtggttttg aaacaaatgc cagcacttcg cttttctcgt ctgcactgag catttccagc
    27841 gccgtcagcg cactgatgcc gcccacttca cggctgaggt cgcggccgcc aaggccaatc
    27901 gcgtgagtaa ttccctcccc tgccagcgca atctgcgagc acagctcctg aatcccggta
    27961 ccggaagcgc caatgacgcc aatgttgcct tccggcatca cgttagcaaa agccagcggc
    28021 gtgccggcaa tcatcgacgt accgcagtcc ggccccatca ccagcaagcc tttttcccgc
    28081 gcgcgggttt taagttggat ttcatcttcc agcgtgacgt tatcggagaa catcatcacg
    28141 ttgaggttgc gatccagcgc ctgattcgcc agctccgccg catactcgcc agccactgaa
    28201 atcagcgcca gattggcatc gggtaatttc tgacaggcac tgtcccagcg acgcacctgc
    28261 gtcaacgcct ggctgctgcc tgacccctgc gccagttgtt ttagcgcctc ttcaagctgc
    28321 tgcataatcg cctgcgcgat ccccgcatcc gccgcttcgc tacgaattgc cacgcaaata
    28381 tcgttcggcg tggcgttatt aaaatcgtca tgccagaaac ctgtggtatc taataacgct
    28441 ttattggcgg gcgtacccat cattacggaa acatcatcaa cattttctga ttcgctgagt
    28501 tttcgtgaaa taatcattaa actgaccgaa tcctgaaaac accctttttt aataaaggcg
    28561 tggatcataa caactcctta aaaattgcac ccatggcaat tgcacgagca gtgttaatgt
    28621 ggcgattacg gtatcgatga caacgagtaa ataaggtgat gaatatcaca cagggattat
    28681 taacaaaaga attcaaataa ccgccatgct attacgtgtt attttttaat aagcgtggtt
    28741 gttttaaaaa ccaggttaaa agcgagcgga ttatcaaata aattctaact atattttttt
    28801 gcctgtctgg atcacataat ccagatattt tccctgtatg ttaattgcag tcatgcttca
    28861 caccgtcgtt aaaaaggaag acagatgaaa atcagtcggg aaacactcca ccagctaatt
    28921 gagaataaac tctgccaggc tgggttaaaa cgtgagcacg ctgcaaccgt ggctgaagta
    28981 ttggtttatg ccgatgccag agggatccac tctcatggcg cggtgcgcgt ggaatactac
    29041 gcggaacgca tttcaaaagg cggcaccaac cgcgaaccgg agtttcgtct tgaggaaacc
    29101 gggccgtgct cggcaatttt acatgccgac aatgccgccg gacaggtcgc ggcgaaaatg
    29161 ggtatggaac atgccatcaa aaccgcccag caaaatggcg ttgcggtggt cggtatcagc
    29221 cggatgggtc acagcggcgc aatctcttat tttgtgcagc aggcagcccg cgccggatta
    29281 attggcattt cgatgtgcca gtccgatccg atggtggtgc cgtttggcgg cgcggaaatt
    29341 tactacggta ctaacccact ggcctttgcc gcgccgggag aaggcgacga gatccttacc
    29401 tttgatatgg cgactaccgt acaggcatgg ggaaaagtcc tcgacgcccg ctcgcgtaat
    29461 atgtctatcc cggatacctg ggcggtcgat aaaaacggtg caccaacaac cgatccgttc
    29521 gcggtacatg ctctgctccc cgcagccggg ccgaaagggt atggcctgat gatgatgatt
    29581 gacgtcctct caggcgtctt actcggctta ccgttcgggc gacaggttag ttcgatgtat
    29641 gacgatttac acgccgggcg taatttgggg caattacatg tagttattaa cccgaacttt
    29701 ttctcctcca gcgaattatt ccgtcaacat cttagccaga ccatgcgcga attaaatgcc
    29761 attacccccg cgcccggttt taatcaggtt tattatcccg gacaggatca ggatattaaa
    29821 caacgccaag ccgccgtcga aggcatcgaa attgttgatg atatttacca gtatttaatt
    29881 tccgacgcgc tttataacac gtcatacgaa acgaaaaatc cctttgcgca ataattattg
    29941 agacaggact ttcttatgat tacacatttt cgtcaaacca tagaagaagc gctgccctgg
    30001 ctttcctctt ttggcgctga cccagcgggt gggatgaccc gtttacttta ttcgccggaa
    30061 tggctggaaa cccagcagca attaaaaaaa agaatggccg caagtgggct ggaaacacgt
    30121 ttcgatgaag tggggaattt atacggtcgc ctgagtggca ccgaatatcc acaggaagtg
    30181 attctgagcg gttcgcatat cgataccgtg gttaacggcg gtaacctcga cgggcaattc
    30241 ggcgcgctgg cggcgcggct ggcaattgac tggctgaaaa ctcaatacgg cgcaccgctg
    30301 cgtacggttg aagtggtggc gatggcggaa gaagaaggta gccgcttccc gtatgttttc
    30361 tggggcagta aaaatatctt tgggctggcg aatcctgacg acgtgcggaa tatctgtgat
    30421 gccaaaggaa atagttttgt cgatgcgatg aaggcttgcg gatttactct gccggacgcc
    30481 ccgctaactc cgcgtcagga tattaaagcc tttgtcgaac tgcatatcga acagggctgt
    30541 gtgctggaaa gtaatgggca atcaattggc gtggtgaatg caattgtcgg gcaacgtcgc
    30601 tatacggtga cgctgaacgg cgaatcaaac catgcaggca ccacgccgat gggttatcgt
    30661 cgtgatacgg tttacgcttt cagtcgcatt tgccatcagt cgatcgaaaa agcgaaaaag
    30721 atgggcgatc cgctggttct gacctttggg aaagtagagc cgcgcccgaa tacggtgaat
    30781 gtggtgccgg gtaaaaccac gttcaccatt gattgtcgtc ataccgacgc cgccgtgctg
    30841 cgtgatttca cccaacagtt agaaaacgac atgcgggcga tttgcgatga aatggacatt
    30901 cgtattgata tcgatttatg gatggacgaa gaacccgtgc cgatgaataa ggacctggtc
    30961 gccaccctga cagaattgtg tgaaagtgaa aaactgaatt accgggtgat gcacagtggt
    31021 gccgggcacg acgcgcaaat tttcgcgcct cgcgtaccaa cctgcatgat ttttatcccc
    31081 agcatcaacg ggatcagcca taacccggcg gaacgcacca atattaccga ccttgccgaa
    31141 ggggtcaaaa cgttggcact catgctttat caacttgcct ggcagaaata aggagtcata
    31201 aatgggatat ttaaataacg tcaccggtta ccgcgatgat ttactggcta accgtgcgat
    31261 tgtgaaacac ggtaatttcg cactgttaac accagacggt ctggtaaaaa atattattcc
    31321 gggctttgaa aattgtgacg cgacaatcct ctccacgcca aagctgggtg cctcttttgt
    31381 tgattatctg gtcacactgc atcaaaacgg cggcaaccaa cagggcttcg gtggcgaagg
    31441 cattgaaacg ttcctgtatg tgatctctgg aaatatcact gccaaagccg aaggcaaaac
    31501 attttcctta agcgaaggtg gctatcttta ttgcccgcct ggctccttaa tgacgtttgt
    31561 taacgcccag gccgaagaca gccaaatctt tttatataag cgccgctatg ttccggtaga
    31621 aggctatgca ccgtggctgg tttctggcaa tgccagcgaa ctggaacgca ttcactatga
    31681 aggtatggat gatgtgatcc tgctggattt cctgcccaaa gagttaggtt ttgatatgaa
    31741 catgcatatc ctttcttttg caccaggtgc cagccacggt tatatcgaaa cacatgttca
    31801 ggaacacggt gcctatattc tttccggtca gggggtttat aacctcgaca ataactggat
    31861 cccggtgaaa aaaggcgatt acatctttat gggcgcttat tctttacagg ctggttatgg
    31921 cgtagggcgt ggtgaagcgt tcagctatat ttactcgaaa gattgtaacc gcgacgtaga
    31981 gatttaagtc ataccactcg cctgattatt tttagctatg ttggattttg ccacggttta
    32041 taccgtggct ttttttgcaa catagcctga tgcgcttcgc ttatcaggcc tacatgatct
    32101 ctgcaatata tagaatttgc gcgattttca ggaaggatat ggtactcatg ccgcatccgg
    32161 catgagtact gcactcttat gcgatataaa tcgcatccgc tttaaaggta agggtcagtt
    32221 tttaattccc tgacctattt taatggcgca ggcaatattt cgcgcgctgt tgaagagatt
    32281 ggtttcaccg ctggcgagca cttcggctaa aggtgccaga cgtggcaaaa tgctgaatac
    32341 cgcatcaatg ccgtactggt gcaccacttc cacgccatca cccaatacgc cagcaatccc
    32401 aatcaccggt acattaaact gcttcgccac cgacgcaacg cccagcggcg ctttaccgcc
    32461 tgccgtttgc gagtcaatgc gcccttcccc ggtgatcacc agtgctgccc cctgcaccgc
    32521 ctgctccagg ttgaccgccc gcagtacaat ctcaataccc ggtttaatat ccgcattgag
    32581 gaataccgcc gccgcaatcc ccataccgcc cgcagcgcca ccgcccgcca tctggcagac
    32641 attaatttca gtttgctgtt gtaaaacacg ggcgtaattt tgcagcccct gttcaagttc
    32701 ttcgaccatc tccggcgttg cccctttttg cgggccaaac accgccgccg cgccgcgtgc
    32761 ccctaccagc gggttatcaa cgtcacatgc tacttcaata tggcagttcg ccagacgcgg
    32821 atcgcattcc tccatctcaa tgcttgccac gcgcgctaaa ttgccgccgt tagccaccag
    32881 cacctgaccg tcggcatcaa ggaaacgcac accgagcgcc tgcgccatgc ccataccgcc
    32941 gtcgaccgtc gcactgccgc caatgccgag aataatatgg cgaatgccgt tatccagcgc
    33001 atgacgaatt aactcccccg taccaaaact ggaggccagc aacggattac gcttttcagg
    33061 cgcgaccagc atcaggccac ttgctgccgc catctcaatc accgccgttt tcccgtcgcc
    33121 ggtaatgcca taaaaagcat tcactttttc gcccatcggc ccgcagactt caagcgtcac
    33181 gatgttgccg cccgtcgcgg cgaccatcgc atccaccgtc ccttcgccgc catccgctat
    33241 cggcaaacag atatagttcg catcgggaaa gagggtcgaa aacccggctt taattgcctg
    33301 acaacatttt tctgcactca agctctcttt aaaagagtct ggcgcaatga caatcttcat
    33361 tatcaatcct ttttcaacaa attattcgat gcgttcccac ggaataaggt tatccataag
    33421 aacagccagt aaaatcccga ccaataagcc gttactgagc agaggacgaa gcgttaatgg
    33481 aaggtcttgc agatacacag gcggtaatgc cattaaaaat atgccgacaa ataacggcaa
    33541 tgcgagtcga taaatattgc gagcggtaaa cgttatttgc tggctaaaca ccagcgcgga
    33601 aaagagtaaa ggtagataag aaaccagcat gaccgcacta ctcacgggta aagggatact
    33661 gcaaaacagt cgcgtgagtg ccggaaccag cgccaccagc aggcaaataa cgctgccata
    33721 aataaacgaa cgccgcgtgt aatcgccagt ctgggttaat aaaccaatgg atgaaacaaa
    33781 cggtgaaaat ggaattactg ccagcggcac ggttatcagt gtcataaatc cggtcgccac
    33841 aaagctacga cgataacgcg tattccccgc gccctgctgc ggataaaaaa catccgtgcc
    33901 ccgaatcgca ccgtaggtat tgctgatatt caccagacct gtaatcacct ccgtcagaat
    33961 aatccccggc gacaaagcac cgccactgcc gagcgggaac cactgccagt gcaactcacc
    34021 ggagagcgaa tgcgaagaag gaaagcaaaa gtaccacaac aaccagccgg ttatggtgcc
    34081 gaccagcagg ccataacggg caaaacgttg cggcagaaag ataatcatcg ccagtaccag
    34141 gcacatcacc gccaccgaga gcgcgaacgg cggtaactga attttaaaat tcgggtcggc
    34201 tatgccaaac ggcagcccga gcataccttt gaaaaagata gtggtcagct gcgcgcccag
    34261 catcaacata aacaagacca tcaccgacgg cgtaaataac cgcgccaggc gatgacctaa
    34321 tccgctaaaa ccaatcaaca tcgtcagcac gccggagagt gcaatcccca ccgccaggct
    34381 ggtagcgata tcgttgatcg gtgtcccgcg cgatgcttcg ccaagggtga tagttaggat
    34441 ggttccccac cacaggcccc ccggcccttc cataatagcg cgacgatgac cgcaaaacgc
    34501 ctgagcgaag caggccagtg cggtagcaag aaaagcatat tgcgtgagcg taagcaggct
    34561 actttgcggc aactgaaaag cagaaagtag cgtaggagga accacaaccg tgttgcaaaa
    34621 aataaagaaa aaccactgaa atcctgataa caggctttcg cggctaactg caaaattgaa
    34681 cataaacaca ccttaaccgg agaatgcccg ccgcaaggac gggcattgca ggggccagat
    34741 tactgctgat gtttaaggat aaattgacct ttcggcgcaa cagggaagcc ctgttcgatg
    34801 tcgtaaatca catcaccacg taagatggtt ttcgtgatac gcgcgccaat ggtacggcca
    34861 acatacgggc tgactttgtg gcgatattcc agatcgtcat tggtaagaac atagctgcta
    34921 ttcggctgaa tgaagacgaa gtcggcatct tttcctgggg cgatacggcc tttttgctgc
    34981 agaccaaaaa tatctgctgc gttagtcgcc attaatttgc cgaacattgg cagagacatt
    35041 ccgcgtttct gtaccgcttc atcgaacatc acgtccatac agttttgcag accggcaata
    35101 ccgccccatg cttccatgat gttgccggct ttcatttccg gagggcatgg tgagtggtcg
    35161 gaaaccaggc ggtcgatttc accgttaaac agtttttccc acatgccttt ctagttttcc
    35221 agatcgcgga tcggcggtga acacttcgcc agagtaccga tttcttcgaa ctgatcggta
    35281 tccagtacga agtaatgcgg gcaggattca caagtaacgt cctgaccttc ctgacgtgca
    35341 cgagtcactt cttcaacgcc ttccgggctg ctgatatggc aaacgtgcag acggcaaccg
    35401 gcaactttcg ccagatacag cacgcggcga atcgcttcca cttcggtaaa taccggacgc
    35461 gaagccacat agtcatgggc agttacgcga ccttcacgtt tcgcttcttc gcccagtgcg
    35521 tcacagatca gcgcgttttc gcagtgcacc agcaccggct gccccagttc gcccagcttc
    35581 tgcgcacctt tgaagaactg ccagtcgttt acatcacgga agtcgttgtc gataccgcga
    35641 tcgccacagg tcgcaacgaa gcatttgaag ccgacaacgc ccacttcatc cagctcatgc
    35701 agacggtcga tgttgtaaga caccaggcca ccgagttgtg ccgcatcgat agtcagcttg
    35761 cctttagcgg catcgaactt cagttcaatt gaagcgcggt caaccgttgc aggcagctgg
    35821 ttgagcggca tttcgatcat ggtggtgata ccaccttttg ccgctgcgcg agtaccggtt
    35881 tcataacctt cccagtggct acgacccggt tcagaaatat gggtgtgcgc atcaaccatg
    35941 cccggcgaaa ccaccagacc agacgcatcc ataacttctt ttgcatcgcc cagatcctga
    36001 ccgatagcag caattttgcc gcctttaacg gcgatatcta caacgcgagc ttcgttttct
    36061 aaaataacgg tgccgttttt aatgattaaa tcaaaagaca taacaaactc cttgtaaaat
    36121 acagattggt ttttaactca agcaaaggcg acgatgacgc cgacaaacca tgaaacacgc
    36181 gataacggtt ccattaaggg aataaactta ccgccaagag ataaaataac ggccaccaga
    36241 gttactgaaa acgcggtgag gttaaaaccg ttatcgtaat atttataatc gccaggtgcg
    36301 gtgtacagtt catcaagatt aatttgccca cgcatcacca caaaataatg cgccatcatg
    36361 acaccaatta ccggaccaag cattccgccg ataatatcga ggaacagata aatactgtcc
    36421 tgattttcca ttaatttcca cgggcagatc agcaggctga taatactggc aatcagtacg
    36481 ccgtttttat aggtcagttt tgtcggtgca atggcagcaa tctgataacc agccggaata
    36541 atattaccag tggcgttagt ggagatagtc gtcatcagaa taaccagtac cgcaaagaac
    36601 gaggcaaaca ggctgtccca acgctgaaca atatccagca cgttccaggt atcagcgccg
    36661 taatgaatac tggctccggc aataatacat acccccgcga ccgcaaacag aatataggcc
    36721 acaactaaac ccagcgtttg ccccagcgct tgctcacgaa acgagtgggc gttttgcgta
    36781 aagtcggatg cgctcaccgc cggtgccgcc cagaccgcaa ctaccgcgtt aatcaccacc
    36841 aggaacaaga atccactgtt ttctgctttc tgaataccgc tcggaatgta gtcaaagatt
    36901 ggaccgagcc cgaccagcga aatcgcccaa atcgccatac cgccgaaaac gatatagatg
    36961 cacgggttaa gaatggcagt gaatttattt aaaactttgc caccgccaaa acctatacca
    37021 acgttgacca gccagaagag taagaaagta attaagcccg gtagagaaag gcctaacaga
    37081 gtgaaatcac caccgagagt caaaaatccc ggccagattt tgccaatcag aatcaagcag
    37141 gccagtgacc ccgcgtaaca ttgcaggcca aaccacataa tggcagcaat accgcctctt
    37201 aataatccgg gaaacagtgc gccacgtacg ccgtaagaag cacgcaggat catggcaaac
    37261 ggtacgccgt atttactgcc cgcagcgccg tttaatacca ttaccgcggc aatgaaaaag
    37321 gcgctgagga taattgccag cataatacta aaggtagaca agccgagaat aaaaaagccg
    37381 ccgaccatca cataattggg aacgttatga accgaaccca tccataaggt aaaatagtta
    37441 aatgttttcc aggtccgctg gctttgcgtt ttcggcaata gatcttcgct atagccgcgt
    37501 tgctggaata gttttctctg atgttccata aatccctctt aatttcacgt atcgaataac
    37561 gttgttttaa tctagctatg aggaaaaaat aaaataaaaa tattattttt aatactgaag
    37621 caacatcttt ggctgctttc ttttttaata ttgctggccg attcattttt tgatatcggc
    37681 catttttatt gcgggtatca ggccagttta tggttagcca ttaattccag cgcctgcact
    37741 aacgcagagt gatccaactg gctgccaccg tttgccgcgc aggtattaaa taactcctgg
    37801 caggtcgcag tgtttggcag gttcagagcc agtgccttcg cactttgcag tgccaggttg
    37861 agatctttct ggtgcagcgc aattttgaaa cctggattaa aggtgcgttt aatcatgcgc
    37921 tcaccgtgaa cttccagaat acgtgaggaa gcaaagccgc ccatcagcgc ctggcgcaca
    37981 cgtaccgggt ctgcaccggc ttttgaagca aacagcagag cttcagagac cgcttcaata
    38041 ttgagcgcta cgataatctg atttgccact ttgcaggttt gaccgtcgcc gttaccgccc
    38101 acaagggtga tatttttacc gagcagttca aacagcggtt taacacgttc aaataccgct
    38161 tcgtcaccgc caaccataat tgacaacgtc ccttcacgcg cgccgatttc gccgccggag
    38221 actggcgcat cgagataatc accacccagt tcattcacct gacgagcgaa acgtttagtt
    38281 tcaatcggtg aaatggagct catatcaaca atggttttgc ccttcagcga ggctttggta
    38341 caaccatttt caccaaacag aacttcttca acctgaggtg tgtccggcac cataataaaa
    38401 atgatgtccg ctgcttccgt tacctggcgc gcggtgtcga cattaacagc accgagtgac
    38461 agtaattcat cagcaaccgg tccaatggtc gtgacatgta attgatgacc ggcacgcgcc
    38521 agattaatgg ccatcggagt acccataatg cctaagccaa taaatcccag tttcataatt
    38581 aacctctttt taaatgtcgc tattcctgaa ttcagacaac acgatcccct gccaacttag
    38641 cagcctaaaa aagcattgtc tgaatagcgt tacgttttaa cggtacggat ccatccagcg
    38701 taaaccggct tccgtggtgg tttggggttt atattcacac ccaacccaac cgttgtagtc
    38761 agaattttcg attaccttaa agagataatc ataattaatt tctccggtcc ccggttcgcc
    38821 gcgatgcgga ttatcggcaa tttgcaggtg accaatttta tcagcccatt gcgtcatagt
    38881 gttggttaat tcaccttcca tccgctgcat gtgataaatg tcatactgaa ttttcaaatt
    38941 gcagcaacca acatcatcaa tcaatttcag cgcctgccga gttccggtga gatggaaacc
    39001 aggaatatca aaatggttaa taggttcaat cagtaataaa atatcttctt tcatcagcat
    39061 attcgcggca taacgcaggt tttctacaag cgttgcgtga atctgttcac tgctgaaacc
    39121 agccggcgtt ttaccgacca gacagttaat atttttatta cccagcgcac gggcataacg
    39181 aatcgctgct gctacgccat cccgaaactc ttcttcacgg ccaggaatac aggcaatacc
    39241 gcgctccccc gccgcccagt cacccgccgg taaattgtgc agcgtatgtt cgagtttatt
    39301 actcgccagc acctgtttta attcttcaat gtcgtagtca taaggaaaca taaattcaac
    39361 gccgcgaaaa ccacactgcg cagctttctc aaaacgggcg agaaaatcat attcaccaaa
    39421 taacatcgat aaattagcag agaaacgtaa cattatttat ctcccttatt catagtgcat
    39481 gaagcaggtt tcagtcggtg catccgctgc gttatcggcg atatcttcaa attccatgac
    39541 gttatccagt tcgctgccca tcgaaatatt tgtcacacgc tcgagaataa cttccacgac
    39601 taccggtacc cgatattgcg ccattaaggc tttcgcctgt tcaaaggccg gtgcaatatc
    39661 ttccggtttg aagacgcgaa tagctttaca acctaaacct tccgctactt ttacgtggtc
    39721 gacgccgtaa ccgttcactt cgctggagtt gatattctcg aaagcgagtt gcacgcagta
    39781 gtccatgtca aaggcgcgtt gtgactgacg aatcaggccg agataggcgt tattcaccag
    39841 cacatggatg tacggaatgt tgaactgcgc gccaacggct aactcttcaa tcaggaactg
    39901 gaagtcgaag tcaccggaaa tcgccaccac attgcgtttc ggatcagcgg cacaaacccc
    39961 cagcgccgcc ggaatcgtcc agcctaacgg accagcctga ccacagttga tccagtggcg
    40021 gtctttaaag acatgcagca tttgtgccgc agcgatttgt gacaggccaa tggtggtgac
    40081 ataacaaaca tcgcgaccaa aggctttgtt catctcttca tacacgcgct gcggtttcac
    40141 cggcacgttg tcgaaatggg ttttgcgcag caaagtgcgt ttgcgctgct ggcaatcggc
    40201 gacccattct ttacgacacg gcagacgacc cgctttttgc atctcctgcg ccacttcaac
    40261 cagcagtgtc agcgccgctt tagcatcaga gacaatgccg agatccggac acagcacgcg
    40321 gccaatttgc gtcggctcaa tatcgatatg aacgattttg cgcccttcgg tgtatttctc
    40381 taccgaaccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca tgtcggacgc
    40441 cagcaacgtt gcgttaccgt aacgatgcgc ggtttgcaga ccgaccatcc cggccatcag
    40501 ttcatgatcg tccgggatac agccccagcc catcagcgtt gggatcaccg gaacgctggt
    40561 cagttcagca aactgttgta acagtgcagc tgcgtcagca ttgattaccc cgcccccggc
    40621 aacaatcacc ggacgttcgg cctggattaa catttctaca gctttttcga tctgcatacg
    40681 gctggcagca ggtttgtaga ccggcagcgg ttcgtacatg tcaggatcaa actcgatttc
    40741 cgcaacctga acgtcgaacg gtaaatccac cagtaccgga cccggacgac cagaacgcat
    40801 caggtgaaat gcctgttgca gcacgcgagg caccagcgcc gcttcacgaa ctgtaaccgc
    40861 cattttgctg accggtttag caattgcttc aatatctacg gcctgaaaat cttctttatg
    40921 cagacgggcg cgcggtgcct ggccggtaat gcacagaata ggaatggaat cagcagaagc
    40981 ggaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca gacatacgcc
    41041 gatattccct gccgttgcgc gggtataacc ttccgccatg tgcgaagcac cttccacatg
    41101 acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga acggattgat
    41161 tgcagctccc ggaacgccga aggcggtggt gataccttct ttctccagca catacattgc
    41221 cgcgtcaacg gctctcattt ttgccatttt attcctacct ctattaattg gaaaaatttt
    41281 ccaactttca aattttgtca aaaaaacccg ctccgcagag cgggatgcga cagacactat
    41341 ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct gaccagctca
    41401 ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat ggagatagcg
    41461 gcaacaacgc tacctacatc atcgtaaatt gctgaagcta tgcaattcag acctacaaca
    41521 tgctcttctt tatctacggt atagcccagt tcacgcgctt gttccaggtc cttcagcagg
    41581 gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg cagaatgatg
    41641 ctcatcaact cctcttccgc cagcggataa agcagcgctt tgcccgcacc ggaagcatgc
    41701 agtggcagac gactgcccag tggcgcgcac atcctgacca tcgatttaca ctctaactga
    41761 ccaattaata ccgcttcatt gccgttacgg atcgcgacat tgaccgtttc gccggaaagt
    41821 aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt atggatgtac
    41881 gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaactg actgtcctga
    41941 tagacaaaat ccgctgcctg taaaaccttc agcaagcgaa aggtcgtgga gagcggcaaa
    42001 tccagattga gagaaatatc gctaaccgac gaacttcccc cacttttttc cagatattgc
    42061 agaatcgcaa ttccccgctc taatgcctgt gcgcccttct gtgccacagg ctccgcctgt
    42121 cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac ctacaagtga
    42181 gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt cacgcaaaac
    42241 agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg cgatcgatgg
    42301 tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg ttacgatggt
    42361 aattcactcc ctgttcgccg ttggtaataa acgcccgcag ggttgacagg tctggtttgt
    42421 cgtcacctaa cgctacgacc accacaaaca cctcaccttt catcgggata aaggcctgag
    42481 tacccagcgg atgacgttcg agttcgtgaa tggtcagcgg cagattcgcc ggttgcgctc
    42541 ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc aaatcgtggt
    42601 aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgcgtt tcgattacgt
    42661 cgccataagc actaaaggct tcctgactta acggtaatac ctgaagtttc atctttttgt
    42721 ccttgtaaca taattccgtt caaccagcgc cgaccggtca gcttggtttc aataacaggc
    42781 aatctaaaac cttaagagaa caacatcaac acaaaacttg gaatattttt ccaaaactag
    42841 atttagtcca caaattgaac agggttatca tgtaaaaata ctgataaatc agaggataag
    42901 aaaatcacgc aacttgcgga tttaaaaggg gatacctgaa gatgttcgat ccagaaacct
    42961 tgcggacttt cattgcggtt gctgaaacag gaagtttttc aaaagcggca gaacgattat
    43021 gtaaaaccac ggcgacgatc agttatcgca ttaaacttct ggaagagaat accggagtgg
    43081 cgctgttttt tcgcacgact cgtagcgtaa cgttgacagc ggctggcgag catctacttt
    43141 cccaggccag agactggctg agctggctgg aaagtatgcc cagcgagctg caacaggtga
    43201 atgatggcgt ggaacgccag gtgaatattg tcatcaacaa cctgctctac aacccccagg
    43261 ccgtcgccca gttgctggcg tggctgaatg aacgttaccc ctttacccag tttcacatct
    43321 cccgacaaat ctatatgggc gtctgggact cgctattgta cgaaggtttt tcactggcta
    43381 tcggcgtcac gggaaccgaa gcgctggcaa atacctttag tcttgatccc ttaggatcgg
    43441 tgcaatggcg atttgtcatg gcggcggatc atccgctggc gaacgttgaa gagccgctaa
    43501 cagaagcgca gttgcggcgc tttccggcgg tcaatattga agacagcgcc cgcaccttaa
    43561 ccaaacgcgt cgcctggcga ttgccagggc aaaaagagat tattgttcct gatatggaaa
    43621 cgaaaatcgc cgcccatctg gcgggcgttg gcattggttt tttgccaaaa tcgctttgcc
    43681 agtcaatgat cgataatcaa caactggtca gccgggtaat cccaacgatg cgccctcctt
    43741 cgccattgag tctggcatgg cgcaaatttg gcagcggcaa agcggtagaa gatattgtga
    43801 ccttgtttac ccagcgcagg ccggaaatca gcggattttt agaaattttc ggcaacccac
    43861 gcagttaaaa atcaagtttc cttgcgcgtt ggcgcgtaaa atagcgcgca agctgatttg
    43921 aggttcactt ttaactatgc aagagagaca cacggaacag gactatcgtg ccctgctgat
    43981 tgctgatacg cccattattg atgttcgcgc ccctatcgag tttgagcaag gcgcaatgcc
    44041 cgccgctatc aatctgccgt taatgaataa cgatgaacgc gccgccgttg gcacctgcta
    44101 taaacagcaa ggctcagacg cagcgctggc gctgggacat aaactggttg cgggtgaaat
    44161 tcgtcagcag cgcatggacg cctggcgggc agcctgcctg caaaatccgc aaggtattct
    44221 ctgctgcgcc cgtggcggtc agcgctcaca tattgtgcaa agctggttgc atgcagcggg
    44281 gattgattat ccgctggtgg aaggcggtta taaggcactg cgccagaccg cgattcaggc
    44341 gactattgaa ctggcacaaa aaccgatagt gctgattggc ggttgtaccg gcagcggtaa
    44401 aacgctgtta gtgcagcaac agccgaacgg tgttgatctg gaagggttgg cgcgtcatcg
    44461 cggttcggcg tttggtcgca cgttacaacc acaacttagc caggcgagtt ttgaaaacct
    44521 gctggctgcc gaaatgctaa aaaccgacgc ccgtcagaat ttgcgcctgt gggtgctgga
    44581 agacgaaagc cggatgatcg gttcgaatca cctgccggaa tgcctgcgcg agcgaatgac
    44641 tcaggcggcg attgcggtgg tagaagatcc gtttgagatc cgtcttgagc gcctgaacga
    44701 agagtatttc ttgcgtatgc atcatgattt tacccacgcg tacggcgacg aacagggctg
    44761 gcaggaatat tgcgaatacc tgcatcacgg actttcggct attaagcgtc ggctggggct
    44821 acagcgatat aacgaactgg ctgcaaggct ggatgcagca ctgacaacgc aactcaccac
    44881 cggcagcacc gacggtcatc tggcctggct ggtgccgtta cttgaagaat attacgaccc
    44941 gatgtatcgc tatcagcttg agaaaaaagc ggaaaaagtt gtctttcgcg gtgagtgggc
    45001 ggaagtggcg gaatgggtta aggcgcagta atgagggacg tatttgtatt agtcgcgact
    45061 tgatctgaca catggccttg aaaggttgag agttaccggt tttgatatga gtgtcgaatc
    45121 cttatccaaa acaagaggta actctcatgc ttcatactgc caatcccgtc atcaaacaca
    45181 aagccggttt gctcaatctg gctgaagaac tcagcaacgt atcaaaagcc tgtaaaatca
    45241 tgggcgtttc gcgtgatacg ttttatcgtt accgcgaact ggtcgctgaa ggcggtgtgg
    45301 atgcgcagat taatcgtagt cgccgcgctc ctaaccttaa gaaccgtacc gatgaggcaa
    45361 ctgaacaggc tgttgttgat tacgccgtcg ctttcccgac acatggtcag caccgggcca
    45421 gcaacgaact gcgtaaacag ggggttttta tctccgacag tggtgtccgt tctgtctggt
    45481 tactccataa ccttgagaat ctcaaaagac gatactgaac aaattttatc aggtgacgtt
    45541 ccgtaaaaag ttgtatggag ttctcgatgc agtcggcgcg actatcagta cccccgctga
    45601 accctggact atcgggccgc cgtactgggg catgtcaccc tggcgtgccg ctggttttcc
    45661 gctaatggtt tatttcctct ttctttcccg gcaagaattt attttaatcc ggaaggatta
    45721 tttattttca gatagggact cacattaaac acgtaacatt ttaattaatg catggctgaa
    45781 tatgtattac acaatgtcag atatcgtaaa agaccagtgc aattctatgt aaactctctg
    45841 actgttcatt ttatttgttg tttcagggtc ggaactcacc ctcaataaac aactgaatac
    45901 gagtagatat tcatcgtctg agctatatgg ctttacacaa tagccgacat gtcttttctc
    45961 agtcaggaat tttacttttc ctcactacga agttgacgac ttcgatatgg gatagactct
    46021 taattcaagc aattactctg cacaagcatc aggcaatcca gctccaatat ttgttataaa
    46081 ataatgcgta acattaattt caccaacaaa aaaaggttta caccatcccc ttgccctggt
    46141 atcttaccat atttacacca cacttttttt gtatctggat cattctttga tacgattttc
    46201 gccacttgtt ccctggccct cactgtcaat gttttcttaa acctcccccc atcatcattt
    46261 gttagggaaa aataagaaaa aaataatcat cttctacatt tgtgtatatt gatatatcca
    46321 ccgctacgtg cggaaaaaaa tctgggagcc atgtaaaaac tccatcattt tctggtattg
    46381 ccatatagtt tatgtaatgt ttacttacaa gagattcatc tttgtataaa taaagataag
    46441 taattacgca taaaacaaca atgattataa tagcaataat aaatattatc atctttgata
    46501 gattacttga gatagccagc atcttgtaaa gcctttatcg tttctttatg ctctggatta
    46561 atataatcac tacatctatc tgagcaatct gttgttgatg gacatgtcaa cccatggtca
    46621 tttacagcca agtctttttt attatcttca atcatttcag aatgggatag ctttactttt
    46681 ctgccgtaca taccgaacag atttaacccg taatctctaa tctctttttc ataacccagc
    46741 cctttcgccg atcggctaac accagcgtca tttaatttag acactcgaca aaatgccata
    46801 caatggaaaa attgatcagt cccacctaaa tttaatctct tcatatcttc ataagtatca
    46861 gataatatat cccatgctct ttgatggttt agttgatctt ttcttcttat tagcgctaca
    46921 tctgcgggac ttaaccctaa tggatcaata ccattcaccg gattcagcgg atacgcatac
    46981 agactccatc ccccctccag ccctatcggg tcctgagtga tataccgccc ctgcaacgga
    47041 tcgtagtacc ggttccggtt gtagtacagc cccgactcct tatcatactg ctgccccggc
    47101 agccggtacg gctggtgcag gtgatgcggg ttctcctcat tcagctggtt gccccattca
    47161 tcatactccc cgctccacgc cgtattgccg tcttcgctga tgagcgccag cggcaggccc
    47221 cggtggtcgc agtggtaaag atgaactttt ctcgccggtg tgtattccgg ctccacctgt
    47281 ctggccagtt gctccaccgt cagcccgcac tgcgcaagcc acgcccggct ttcactgctc
    47341 acgcggtctg cccggatttc ttcctccagt ctgtccagca gccgcaccag ttcagccgga
    47401 aataccacac cgtgcccgtc ctcgctccct tcctgctgca gcttctccgc caggctgcgg
    47461 cgctgcgctt tctcccgctc accgttgtct gtttcgatgc ggatgagcgg cgcgaagctt
    47521 cccggctcgt ataccgtctg gatacgcgtg gtgtcggtct gtaccgtcgt cagccggtcg
    47581 ccgtcccacc cgtaccacgt cacctccggt ttccgcgaca gcgacatcca tcccgtcagg
    47641 tcacgcccgc gacgccacac ccgtttcccc gtccggcg
//
LOCUS       DABSYT020000025        44836 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.025, whole genome shotgun sequence.
ACCESSION   DABSYT020000025 DABSYT020000000
VERSION     DABSYT020000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 44836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.025"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(273..542)
                     /locus_tag="ID725_RS09070"
                     /old_locus_tag="pgaptmp_001814"
                     /pseudo
     CDS             complement(273..542)
                     /locus_tag="ID725_RS09070"
                     /old_locus_tag="pgaptmp_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(813..1877)
                     /gene="hipA"
                     /locus_tag="ID725_RS09075"
                     /old_locus_tag="pgaptmp_001815"
                     /pseudo
     CDS             complement(813..1877)
                     /gene="hipA"
                     /locus_tag="ID725_RS09075"
                     /old_locus_tag="pgaptmp_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            complement(1993..7421)
                     /locus_tag="ID725_RS09080"
                     /old_locus_tag="pgaptmp_001816"
                     /pseudo
     CDS             complement(1993..7421)
                     /locus_tag="ID725_RS09080"
                     /old_locus_tag="pgaptmp_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            complement(7952..9544)
                     /gene="lsrK"
                     /locus_tag="ID725_RS09085"
                     /old_locus_tag="pgaptmp_001817"
     CDS             complement(7952..9544)
                     /gene="lsrK"
                     /locus_tag="ID725_RS09085"
                     /old_locus_tag="pgaptmp_001817"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HGV7850431.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(9623..10576)
                     /gene="lsrR"
                     /locus_tag="ID725_RS09090"
                     /old_locus_tag="pgaptmp_001818"
     CDS             complement(9623..10576)
                     /gene="lsrR"
                     /locus_tag="ID725_RS09090"
                     /old_locus_tag="pgaptmp_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGV7850432.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            10825..12360
                     /gene="lsrA"
                     /locus_tag="ID725_RS09095"
                     /old_locus_tag="pgaptmp_001819"
     CDS             10825..12360
                     /gene="lsrA"
                     /locus_tag="ID725_RS09095"
                     /old_locus_tag="pgaptmp_001819"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGV7850433.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            12354..13382
                     /gene="lsrC"
                     /locus_tag="ID725_RS09100"
                     /old_locus_tag="pgaptmp_001820"
     CDS             12354..13382
                     /gene="lsrC"
                     /locus_tag="ID725_RS09100"
                     /old_locus_tag="pgaptmp_001820"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGV7850434.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            13382..14374
                     /gene="lsrD"
                     /locus_tag="ID725_RS09105"
                     /old_locus_tag="pgaptmp_001821"
     CDS             13382..14374
                     /gene="lsrD"
                     /locus_tag="ID725_RS09105"
                     /old_locus_tag="pgaptmp_001821"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGV7850435.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            14386..15408
                     /gene="lsrB"
                     /locus_tag="ID725_RS09110"
                     /old_locus_tag="pgaptmp_001822"
     CDS             14386..15408
                     /gene="lsrB"
                     /locus_tag="ID725_RS09110"
                     /old_locus_tag="pgaptmp_001822"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGV7850436.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            15435..16310
                     /gene="lsrF"
                     /locus_tag="ID725_RS09115"
                     /old_locus_tag="pgaptmp_001823"
     CDS             15435..16310
                     /gene="lsrF"
                     /locus_tag="ID725_RS09115"
                     /old_locus_tag="pgaptmp_001823"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGV7850437.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            16334..16624
                     /gene="lsrG"
                     /locus_tag="ID725_RS09120"
                     /old_locus_tag="pgaptmp_001824"
     CDS             16334..16624
                     /gene="lsrG"
                     /locus_tag="ID725_RS09120"
                     /old_locus_tag="pgaptmp_001824"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGV7850438.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            16681..17439
                     /gene="tam"
                     /locus_tag="ID725_RS09125"
                     /old_locus_tag="pgaptmp_001825"
     CDS             16681..17439
                     /gene="tam"
                     /locus_tag="ID725_RS09125"
                     /old_locus_tag="pgaptmp_001825"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGV7850439.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(17443..18357)
                     /gene="yneE"
                     /locus_tag="ID725_RS09130"
                     /old_locus_tag="pgaptmp_001826"
     CDS             complement(17443..18357)
                     /gene="yneE"
                     /locus_tag="ID725_RS09130"
                     /old_locus_tag="pgaptmp_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGV7850440.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(18554..20005)
                     /gene="uxaB"
                     /locus_tag="ID725_RS09135"
                     /old_locus_tag="pgaptmp_001827"
     CDS             complement(18554..20005)
                     /gene="uxaB"
                     /locus_tag="ID725_RS09135"
                     /old_locus_tag="pgaptmp_001827"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGV7850441.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(20232..21650)
                     /locus_tag="ID725_RS09140"
                     /old_locus_tag="pgaptmp_001828"
     CDS             complement(20232..21650)
                     /locus_tag="ID725_RS09140"
                     /old_locus_tag="pgaptmp_001828"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGV7850442.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(21789..22148)
                     /gene="yneG"
                     /locus_tag="ID725_RS09145"
                     /old_locus_tag="pgaptmp_001829"
     CDS             complement(21789..22148)
                     /gene="yneG"
                     /locus_tag="ID725_RS09145"
                     /old_locus_tag="pgaptmp_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGV7850443.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(22148..23074)
                     /gene="glsB"
                     /locus_tag="ID725_RS09150"
                     /old_locus_tag="pgaptmp_001830"
     CDS             complement(22148..23074)
                     /gene="glsB"
                     /locus_tag="ID725_RS09150"
                     /old_locus_tag="pgaptmp_001830"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGV7850444.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(23138..24526)
                     /gene="sad"
                     /locus_tag="ID725_RS09155"
                     /old_locus_tag="pgaptmp_001831"
     CDS             complement(23138..24526)
                     /gene="sad"
                     /locus_tag="ID725_RS09155"
                     /old_locus_tag="pgaptmp_001831"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGV7850445.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            24627..25508
                     /gene="yneJ"
                     /locus_tag="ID725_RS09160"
                     /old_locus_tag="pgaptmp_001832"
     CDS             24627..25508
                     /gene="yneJ"
                     /locus_tag="ID725_RS09160"
                     /old_locus_tag="pgaptmp_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGV7850446.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            25586..26701
                     /gene="yneK"
                     /locus_tag="ID725_RS09165"
                     /old_locus_tag="pgaptmp_001833"
                     /pseudo
     CDS             25586..26701
                     /gene="yneK"
                     /locus_tag="ID725_RS09165"
                     /old_locus_tag="pgaptmp_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            26852..28042
                     /gene="ydeA"
                     /locus_tag="ID725_RS09170"
                     /old_locus_tag="pgaptmp_001834"
     CDS             26852..28042
                     /gene="ydeA"
                     /locus_tag="ID725_RS09170"
                     /old_locus_tag="pgaptmp_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGV7850447.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHLSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(28067..28732)
                     /gene="marC"
                     /locus_tag="ID725_RS09175"
                     /old_locus_tag="pgaptmp_001835"
     CDS             complement(28067..28732)
                     /gene="marC"
                     /locus_tag="ID725_RS09175"
                     /old_locus_tag="pgaptmp_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGV7850448.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            28944..29378
                     /gene="marR"
                     /locus_tag="ID725_RS09180"
                     /old_locus_tag="pgaptmp_001836"
     CDS             28944..29378
                     /gene="marR"
                     /locus_tag="ID725_RS09180"
                     /old_locus_tag="pgaptmp_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGV7850449.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            29398..29781
                     /gene="marA"
                     /locus_tag="ID725_RS09185"
                     /old_locus_tag="pgaptmp_001837"
     CDS             29398..29781
                     /gene="marA"
                     /locus_tag="ID725_RS09185"
                     /old_locus_tag="pgaptmp_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGV7850450.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            29813..30031
                     /gene="marB"
                     /locus_tag="ID725_RS09190"
                     /old_locus_tag="pgaptmp_001838"
     CDS             29813..30031
                     /gene="marB"
                     /locus_tag="ID725_RS09190"
                     /old_locus_tag="pgaptmp_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGV7850451.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(30062..30961)
                     /gene="eamA"
                     /locus_tag="ID725_RS09195"
                     /old_locus_tag="pgaptmp_001839"
     CDS             complement(30062..30961)
                     /gene="eamA"
                     /locus_tag="ID725_RS09195"
                     /old_locus_tag="pgaptmp_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGV7850452.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            31156..32343
                     /gene="ydeE"
                     /locus_tag="ID725_RS09200"
                     /old_locus_tag="pgaptmp_001840"
     CDS             31156..32343
                     /gene="ydeE"
                     /locus_tag="ID725_RS09200"
                     /old_locus_tag="pgaptmp_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGV7850453.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(32384..32779)
                     /locus_tag="ID725_RS09205"
                     /old_locus_tag="pgaptmp_001841"
     CDS             complement(32384..32779)
                     /locus_tag="ID725_RS09205"
                     /old_locus_tag="pgaptmp_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGV7850454.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            32874..33845
                     /gene="ftrA"
                     /locus_tag="ID725_RS09210"
                     /old_locus_tag="pgaptmp_001842"
     CDS             32874..33845
                     /gene="ftrA"
                     /locus_tag="ID725_RS09210"
                     /old_locus_tag="pgaptmp_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HGV7850455.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            33953..34048
                     /gene="mgtS"
                     /locus_tag="ID725_RS09215"
                     /old_locus_tag="pgaptmp_001843"
     CDS             33953..34048
                     /gene="mgtS"
                     /locus_tag="ID725_RS09215"
                     /old_locus_tag="pgaptmp_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGV7850456.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            34048..34152
                     /gene="mgtT"
                     /locus_tag="ID725_RS09220"
                     /old_locus_tag="pgaptmp_001844"
     CDS             34048..34152
                     /gene="mgtT"
                     /locus_tag="ID725_RS09220"
                     /old_locus_tag="pgaptmp_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGV7850457.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(34267..35157)
                     /gene="dgcZ"
                     /locus_tag="ID725_RS09225"
                     /old_locus_tag="pgaptmp_001845"
     CDS             complement(34267..35157)
                     /gene="dgcZ"
                     /locus_tag="ID725_RS09225"
                     /old_locus_tag="pgaptmp_001845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGV7850458.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(35412..35804)
                     /gene="ydeI"
                     /locus_tag="ID725_RS09230"
                     /old_locus_tag="pgaptmp_001846"
     CDS             complement(35412..35804)
                     /gene="ydeI"
                     /locus_tag="ID725_RS09230"
                     /old_locus_tag="pgaptmp_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGV7850459.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            36080..36598
                     /gene="ydeJ"
                     /locus_tag="ID725_RS09235"
                     /old_locus_tag="pgaptmp_001847"
     CDS             36080..36598
                     /gene="ydeJ"
                     /locus_tag="ID725_RS09235"
                     /old_locus_tag="pgaptmp_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGV7850460.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(36643..38688)
                     /gene="dcp"
                     /locus_tag="ID725_RS09240"
                     /old_locus_tag="pgaptmp_001848"
     CDS             complement(36643..38688)
                     /gene="dcp"
                     /locus_tag="ID725_RS09240"
                     /old_locus_tag="pgaptmp_001848"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGV7850461.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            38825..39571
                     /gene="ydfG"
                     /locus_tag="ID725_RS09245"
                     /old_locus_tag="pgaptmp_001849"
     CDS             38825..39571
                     /gene="ydfG"
                     /locus_tag="ID725_RS09245"
                     /old_locus_tag="pgaptmp_001849"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGV7850462.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            39660..40346
                     /gene="rspR"
                     /locus_tag="ID725_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /gene_synonym="ydfH"
     CDS             39660..40346
                     /gene="rspR"
                     /locus_tag="ID725_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGV7850463.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            40523..40726
                     /gene="ydfZ"
                     /locus_tag="ID725_RS09255"
                     /old_locus_tag="pgaptmp_001851"
     CDS             40523..40726
                     /gene="ydfZ"
                     /locus_tag="ID725_RS09255"
                     /old_locus_tag="pgaptmp_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGV7850464.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(40762..42222)
                     /gene="ydfI"
                     /locus_tag="ID725_RS09260"
                     /old_locus_tag="pgaptmp_001852"
     CDS             complement(40762..42222)
                     /gene="ydfI"
                     /locus_tag="ID725_RS09260"
                     /old_locus_tag="pgaptmp_001852"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGV7850465.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(42311..43594)
                     /gene="ydfJ"
                     /locus_tag="ID725_RS09265"
                     /old_locus_tag="pgaptmp_001853"
                     /pseudo
     CDS             complement(42311..43594)
                     /gene="ydfJ"
                     /locus_tag="ID725_RS09265"
                     /old_locus_tag="pgaptmp_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            43726..43968
                     /locus_tag="ID725_RS09270"
                     /old_locus_tag="pgaptmp_001854"
     CDS             43726..43968
                     /locus_tag="ID725_RS09270"
                     /old_locus_tag="pgaptmp_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGV7850466.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(44018..>44836)
                     /locus_tag="ID725_RS09275"
                     /old_locus_tag="pgaptmp_001855"
     CDS             complement(44018..>44836)
                     /locus_tag="ID725_RS09275"
                     /old_locus_tag="pgaptmp_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HGV7850467.1"
                     /translation="DVSPALVSKVTDAVMEQVVEWQNRPLDAVYPIVYLDCIVLKVRQ
                     DSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTELKNRGLNDILIACVD
                     GLKGFPDAINTVYPEARIQLCIVHMVRNSLRFVSWKDYKAVTRDLKAIYQAPTEEAGQ
                     QALEAFASAWDSRYPQISRSWQANWTNLAMFFAYPADIRKVIYTTNAIESLNSVIRHA
                     IKKRKVFPTDDSVKKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT        11395 a        11458 c        10473 g        11510 t
ORIGIN      
        1 tcacaaggac acctaatcac aacaagttta ttgctgcact tgttgaagag agtatttgca
       61 aaaacatttt atgtcgctaa ataacatgac gatttcatta tgcatgttat ttataaaccg
      121 tgactaaaca gaatattatt gcgaacaagg gaagtgtcag gaaaggtcaa gggctactaa
      181 tggtttatat ggctataaat cattagctat agttaatgtt tgcctggtgt aactaacacc
      241 gaattgctct aaacagataa gtgcagggtt tattaaagct gaccgacaga ctttcttaac
      301 ctggtaatat ctctcttagg tgactctcca aacatccgca aatattcccg tcggaaatgg
      361 gataagcttt cgtaaccgac agcataggct gctgtggtga catcgtaatg ttcattcagc
      421 atcgttcgcc tgacttcatt gagacgtagc catttctgat accggagtgg actcattccc
      481 gcaagctgcc tgaagtgttg atggaaagag ggcgtgctca tttgcacgcg gtttgccagc
      541 ttgagtcttc aacgctggga agatgcggca aacggcaaat cggcatacca gccagcctat
      601 atccgtcatg cacaggccaa ttatgaaaga ggagaagctt aagttctgga atcctgaacg
      661 aataacgact accggaaaat cacttcgtat cactcagata cgatttaaca taatatacat
      721 tatgcgcacc aatataaacc aagggagaat ctagtcgttg gcggtcatga ttgtcctgct
      781 cattaacaac gaccaaaccc catatcttac tcttacttaa taccgtattc tcggcttaac
      841 cgaccgtgca acctcaacac attggtttca actgccgtca ccacgttctc tggaaaatct
      901 gtcggtaatg aagtcttcac gttatccagt gctgccggaa tcattctggc aaagtcactc
      961 aggatttcat gcatctgcac ttccgggaat ctcagcacct ttgctgtcgc caaaaaatgt
     1021 cgcggataaa ttttatcgat tgccgttttt ttgcctttgg atgcgttaag ccccattgcc
     1081 agtttgagat cgctgatgtg tattcccgta ccgccaagga ccggaaatgc tgaaatgatg
     1141 tcgtaaaatg gcgtgagtcg ataactgccg ccagcctgaa taaatacgga gaagtttttt
     1201 gcatggccat ccgttgcgcc aatcaaccac tggaagacct ggaatttcat aaaatcataa
     1261 cgatcgcgaa gcgcctcgct gaaccccatc aaaaaagcca tgatccgcgc gatgcctggg
     1321 cctccatctg attcatattt caccgatgaa ggtaaaccga atgtctgaca catatcctcc
     1381 tgtggcaagc gaagtaaaac cgttcgctca gcattccaac gcctgtcaaa acgttcgacc
     1441 gctaacgcgc gcacatttcc cgctttaatg atttctgcgt ccgggacatt caacccaagt
     1501 tctttcgcca gcagcagaca gtaatactca ttatcaacgc tttggctgag atcgagcgtc
     1561 gcattgggct gcctgatttc cccaatcggt aatttaatga tgtgcgtcgt cggcgttatt
     1621 cctttcggaa tgcaccagtc attgcctatt ctgagcagtg ctgtcttctc ctgtgcgcca
     1681 gcaaccgaga tgcgaaagtc attttcttct ctaatcatgc ctagcgggat atctgcttta
     1741 taagccgtta atacttcttc aagtctgact tcagtaagct tttcccatgc cattatcgga
     1801 cgcgttacgg tttcgtcttc gggtattaac gtcacggcac caacgctgtc tcgccctatt
     1861 tctgacaata aatcaaactg tacgggataa acgctgcgat atccgcgatc gcggataaaa
     1921 gggaggcttt aaaagccttt tgaagttggc aatattaagc cgcatgccat ctcgacatgc
     1981 ggcttatact gtttaccagc tatatttcac tcccagcatg ccctgagtat cgctatagcc
     2041 tttatcacct agttgcacac cgacattccc ccacaggcta aggttgttat ttactttcgc
     2101 ctcaaccccg gtacgtactt caccgagatt tcgcgcacca tcacggctta cggtttgacc
     2161 attcatcttc acggcgtaga ctttgctatt gttgatccag ttcgcttcaa tgtaaggctg
     2221 gaactcacgc tgtttaccat cgtcacgctg gtgatggcta ttcaggtagg ttttcacccc
     2281 aagtcgcgtt tgcacgtttc cgtcgccttc cgtttcaatg cgcgttccgt ctttccgggc
     2341 atggtcagaa tctttcacac ccatccaggt gatttgcgcc tgtggctgga cgtaccaggt
     2401 attcagcgtc ccttcgctgc cgctacatgt tcccgcttca aaggtatacc caccctcaac
     2461 agaggccgtc acaccgcgag aatcatagtc gtcagcagaa cggttatcgg aactgacgct
     2521 gttatcaaac cagttataca gcgcccagct gtcaacataa gcgccggtct tattcgcatc
     2581 gttctgatac caggtcgcgt acagcccagc gctgtaaccg ctgatgcgcc catccgattt
     2641 ataacccaca cgattactct gagtattact gtgctgattg gcgtagcctg ccatcacgcc
     2701 aagatgccag cgatcctgcg cgttgctact ccactgcgcc aaatcgccgc ctagctgcaa
     2761 tacatagcgg ttagcctgag tatttagctg gccgtctccg gcactggaac gttcgtgccc
     2821 cccgacatga cgcatccaca tactgcttgc tgaatcctga gaatgcagtg aatctgtata
     2881 ttgcggctca cccagacggt cgtgtaagcg atggctaaac agcgagttgg ctgcggcaat
     2941 gttgctgata tagcttccgg cctccgggcg ataaactgat gggccttccg gatccacaac
     3001 agggggatta ttgatgggat cgggtgtatc cgctggcgtt acgccgtccc atttactggt
     3061 cagataccag tttttctcgt cattcccctt ccctttagcc agcgtgtaga cgtaagcccc
     3121 agcttcgaca gttccggtgg tcagcgcgaa attacctgca gaattaccgc caacctcaat
     3181 gagttcaata ccgttgaccg tttgcgcacc gacgccgcca atgttatcaa cccgaacgcg
     3241 agtgttccct tgggtatcgc ctttcacgtt catcttatcg gtgggtgaat tatcgccgcc
     3301 cagcgtcgcg ttgaacgtca tcaggccatt gtttccggta tagttgccgt taacggtcag
     3361 tacattcccc ggttttcctc cttcactgtt catttgaacc gtgccgccat tctccaagtt
     3421 gccgccgatt cgccgattgt ggttttagcg acacgcagtg tccccccaga cataacgttc
     3481 acatcacctg cagtggtgac atttcccgac atcacaccgc tgttatggat attaatgtgt
     3541 tgacttgccg tattaatggc agagtcggaa ccgaaagcaa tttccccccc cacgatatcc
     3601 gtcgtaccct gatacgccga ggaatcgttg ttaagagtca ggatcccggt acccagtttt
     3661 gtcaaatcac cgctaccgct gatactgtgc gtcatcgccc agctacgctc agtggagatg
     3721 gtgagcaggc cattattgat aacggaagca tcacccaggt ttttctgctc gttgacgctg
     3781 agcgcgctgc ctgtatcaat gttgaattgc cccacaaagc ggctattatc gccatccagc
     3841 tctacatctg tcctggcggt tgcgctcacg atacccttcc cgcttatgct attacgcagt
     3901 tcgccagtca cgttttttag cgttaacaga ccgtcattgg caatatttcc tctgcctaac
     3961 ccctgcgtat tatcgaggct gacttccgca tttgggctaa cgctggttaa cgcattgtag
     4021 cgtgcattaa gcccctcgac agccagcgtt ccccctgtaa cattcaggtt tccgccacct
     4081 gtcagttcac cctgactgat cccaccttta tttaccgtca gcgacccttc tttgaacaaa
     4141 acagtcgaac ccatctgccc ggtgaatgtc tctaccgtct gcgtcgatcc attaagatcg
     4201 acgatggctg cgttgctgat gttcagttcc cgggtgttgc ccagcgcgcc atccgcatcg
     4261 gtacgtaatg tccccatctg aacgtaggtt gccccctgat agtcgttctg accgttggaa
     4321 agcgaaacct gtcgcaccgt attgattgcc agatcccctt caccaccgat ttttgccgac
     4381 atatcggccg ttgcgccata ggctccgcca tgttcggcta acgtcagctt ttgccctcca
     4441 tggatgttca gcgctttcag cccatagtta acgtacaaac catcacctgg tgcggtcgtg
     4501 aggcggaatc cataatcacc ctcggcgacc accgttccgt tttgcgcaat gtgtaacgtt
     4561 ttgtccgctt ccacctcgtc gccctgtaaa tcacgtaacg ttaatgagcc acccgaccca
     4621 ataaccgttt gcgccttcac cagttgaacg ccgacatggt tatcatcgtg ttccagcaaa
     4681 ttgagcgtcg tcagagggtt attcgccata ggatcattcc acggtttagg cacgccgata
     4741 agcacgtcgc ccgtcccgtt tacctgatag ttacggcctt tccaggtgta gtcactcgcg
     4801 ccgacaacca gcgtatcgac gctgatatat ccctctgcaa gcgtcgcagc aggaatatcc
     4861 gtatcgaaaa tgagcgtacc gccattcatg gccagtccac caatggattg ctctcccacg
     4921 tttaccgatg tggtattttc aatgtcagac tgcaacatcg cgtgagtaag cgcagcggtg
     4981 ttgtcgcgtt ccagagtgaa ggtactgtct ttcagttggg caacaccggc gaattcagtc
     5041 cctgttgcat gtgtaaagcc aaacatcttg tcggaggatg acagctgaac tcgcatcaag
     5101 ccatcgcctt tgagcgcatt ggtcagcgtg taatctcctg cactgttcgt tgatgcgaga
     5161 atgtcgagcg taccggggct tgcaatggtt acttcactcg accccatgcc cgttacccca
     5221 tctgccgtat gagacgcaat ggtcaggagg ccgtcattga ccagcgtttt accgctataa
     5281 gtattccctg cattgaacac cgtatggcct gcatcatgga tcacttttcc gtctttatcg
     5341 tcacctgtaa tcagcatatc gacctggtag ccggcatcac tgttattagt atgattaaag
     5401 acaaaaacac cttcgccaga gccaaactct actttcgtcg cgttggtgat aaatccggca
     5461 tctgccgccg cctcaccgtg agctgcacca atgtttagcg ttcctacagc aggttcaaaa
     5521 acacctaagt aaacttctcc accttcgaca ttcagcgtgc cgctattcgt cagggttaac
     5581 gtacccgtac cagatgtccc tacgtacata ttgaatgttt caagaagaga gttctgccca
     5641 tccaccctca cgtcgccctt acttttgtca ttgagagcta tctgtgtatc acgcgcttta
     5701 actatacctc cggtggtaat attcagttca cccgtgccta atacaccgac ctgtagcaat
     5761 tgggcgttag tagaagacgt ctttaaattc cagagactat ccgttgaaat attaactatt
     5821 cccttaccgg aggcatttcc tccgataaat ccatagcccg ttgaattaag ggtggcacca
     5881 ttactgacat ttacgatacc gctaccaaaa ttaccgacat tgatacccac agtatcgaac
     5941 gacgatgtcg catccatcag gttcagtgtg ccgataccgc ttgctttatc gcccaaaaaa
     6001 gtaatatttt gcgatacgaa cttaccgcca tcgctaatat tgaggtttcc gacgcccgca
     6061 acaccaatgg tggtatagac attcttattg acctcccata tcccccccgt ggtgatgcta
     6121 accttcccga cgccggtttc tccataaccg agagagcttc ctccgttgct gacaacaagc
     6181 ccctcattac tgatattcat ttcgccgtgg ccgtcataac caagattagt tccgagatta
     6241 gtgataacgg agttcttatc cttaacagta aggttgcctg tgcctgtttc tttcatcccc
     6301 gcagtgataa ttcccgaatc tactttacct tcacgcgaga cattaagttc accgtcgcca
     6361 gcatcaccga tatagatata gcggaaagct tcgcccgttc cgaggaaatt ccaatgccct
     6421 ttatcggtca cgttgacgac accgtgggaa ccgtcctgaa cgcccaccaa tgaatattct
     6481 ttgttattaa ttagtccatt attggaaata ttgactgcgc cattaccgaa atatccatta
     6541 tagagtctgc gtaccgtgat gacagagtct tgatcctgca cattcagggt tccgacaccg
     6601 gtggcattgc caccgataat cgtattttca gccgtaatca gcccaccctc gcgaatagtc
     6661 gcctccccag ttccttgatt accaatttga aattcaattg aggaatcatt attttttatt
     6721 agccactcgc cacccttttc aacgacaacc ttaccattac tgttcgcttg atatcctaaa
     6781 atggcgacta ttgaactcgt gacgtaaccc ttatccgtaa tatttaatga gcccgtaccg
     6841 taactcccta tttcgaataa ttcggtcgtc aaaaccgagt cctctccctc aacattaacc
     6901 gtcccgacgc ctgcagcttg agtacctaat cttaaatagc ctccatcgac gtgacccttc
     6961 tgtttaatat tcagcgtccc cgttccggat tgacccacat ttaaaggcct tgcattattt
     7021 ccgctatcat acaatcgcca ggtgccaccc aaaacattaa cggtaccctc cgaatcctca
     7081 ttaccgccaa tgacgctggt agtaatagtg gttaaggagg cattaccgcc agccaaaata
     7141 ttaagcactc cagtgccata ccagccaacc aggtaggcat cataagcaac atcagtatca
     7201 atattggtaa tctgaccatt atcaacttcc agactcgcac cagacactga accgcttagt
     7261 gccagcaaaa cacccagcgt caatctactg aatttcgttg tcagtccaga ggactttcgc
     7321 aaattaaccg ttgatgtttt acctaccctg cgagttaatt ccgagcaggc ctgaaatacc
     7381 tgtagagtgc aattccatat cacgcgatag attctattca tataaacgct ccaaatacaa
     7441 acaatacagc cacgaaagag atgagtcgtc agacattaaa aatgaaattt attaaattgt
     7501 cagaggtctg tattgagtgt tagttggagg tgggaaagtg tcaatttggc taacttgcta
     7561 atactttcga attgatagat taaaaattgc atagttctta ttttatttaa aaatgaacta
     7621 tgagttattg ttgcttaata agaaaactgt ttctttcagt aggaaaatta tcatcatcat
     7681 atttaacaaa gaatagcacc aattgctaaa aatcgaagtt tattaaaccc cttttgcatc
     7741 cattaatgtc tttaggaata ttcgttataa aataagggtt atgcttaatg ctttaagaaa
     7801 aatagcaatt tccccttgaa tatagtactg gtgatgaaac gatgaatctg caagctggca
     7861 tttaacaagc cagctctaaa agaagggaaa taagaataac tatactcaaa aactaacagc
     7921 cacggtcatc atgatgtggc tgtcaatgaa actataaccc aggcgctttc cataacgacg
     7981 tcgtcagtcc atgatcaacc agccccagct gatcctgata aactgcctgc cacttatcgc
     8041 gtgaatcctg ataaagttca tgcttttccg ggtctggtgt gtgcgtccgt tcccagcgaa
     8101 ccaggcgttc tccggtttct gccattgatg aaaaaattcc ggcaccgacg ccagctgcaa
     8161 tggcacatcc taatgcagtg gcttctttga ccaccggaat attgacgggt aatcccgaga
     8221 catcagcgag aatttgactc cataatttcc cttttgaacc tccgcctgca aagactaacg
     8281 atgaaggatg aatagtcgaa aaatcagcaa tttgctgcaa gttacacgct gatacaatcg
     8341 ccgcattttc ttccagcgca cggaacaatg tcgctttgtt acatttatcc ggatcaatgg
     8401 acaaattaat aaaggaaggc gcagcgtgat accaggtttt aaagcgcatt ctgtcggaga
     8461 agatcggcat tacgccccac gacccaggcg gcacccgact ggccatctct tccagcagcg
     8521 tataggtgtc gatgcctaaa cgttcggcaa tcagtttttc ttcggcacag aaagcatcgc
     8581 ggaaccagcg catggtgagt ccggtaaaaa agcttataga ttcagcttgt accatgccag
     8641 gaataacatg aggattaacg cgcacgttca tttctgggtc tgtcaccggc gcggctaaat
     8701 ttacaacttg ctgccagaat gtgccgccaa gaaccgcggt ttgtgccgga cgcacaacgc
     8761 ctaaccccag gcaaccaagc tgcacgtcgc ctcctccaac gaccaccgga gtgcccacct
     8821 tcagaccgca gagttccgcc gcttgtgaac ttaccacgcc cagcaatgtg ccggtttctt
     8881 tgacaggaga aagaatatcg gcacgcaggc cagccatatc cagcaatgca ggtttccagt
     8941 cacggatggt tagatcaaga agtcccgtgg tgccagcgtt agagggatcc accgcaagtt
     9001 cgccgctgag catataggcc agccagtcgc tgatcatggt gatggttgat gcctgacggt
     9061 aaatatcgga acgatggtgc gccagccaaa gtaatctggg gatggcactt aaagccagtg
     9121 tttgtccggt cgcgcgataa acttcgtttt caaaggtatt gttgtgcagt tctttaagtt
     9181 cgctaacttc gcgcgccgct ctggcatcca cattggcgca ggcccagatc ggtgttcctt
     9241 cattgttata taaaacaatg ccttcacgca tcgaacatgc cgaaacggca gcgatatact
     9301 ccggggctat gccggcgttg tgcagcgcct ggcgcataca ctcacacgcc agttgccagt
     9361 ttttgttgag atcaaattcc atagaaccag gaacgtccgg tactgccaga tgccgccact
     9421 ccgcctgtcc cactgctatt tgattgcctt ccaggtcgaa tatcacagcc cgaatacttc
     9481 cggtgcctgc atccagcgcc atcaggtagt actttgattc tgaaggggta aagagtcgag
     9541 ccattagatt atcctcggct tatgcgttct gtctgttcct ctatacgttc tccatcattc
     9601 ccggtaataa ggtcatgcaa atttaactac gtaaaatcgc cgctgctgtg tcctgatcgg
     9661 taaccagtgc gttgatataa ccgcctttca ttgcagcggc aattgcttcg gctttatttt
     9721 ctccccctgc cacgccaacc cggacgggta tggtcttcag cgcgcttaaa ggtaagccaa
     9781 tcagctcgtt atgtatttta atgtccgtga caacgtcacc ttttgcatca aaaaagtagc
     9841 ctaaaatgtc gccaaccgcc ccttttcggc caatcattaa ctgttcgccc tggctgatat
     9901 aaccggagcg aatgatcgtc gcgtcgtctt gttgactaac ggccccaatg ccgacaatcg
     9961 ccacatccgc tgcttgcgcg gctaacagaa catctttgac acaattttca ttttttagcg
    10021 tacgggcaat gtcagcggag gatgcccgca acggagccgg aataatattc acactgcacg
    10081 ccgcattaag ctgtccgata cccgtcatat aagaaccgac gccaccggag agcgtgacca
    10141 ggcgaatttg ctgtgacgaa ataaaaccac ttaagcgttg cagcgtattc atggttgcct
    10201 cgccaaaacc aatcgccagc atctgttgtg gttgaagtaa actcatcaac atatgcgccg
    10261 cgcctatccc cagtcgcccg ccgacatcag catccgcaag cccagggatc acccggacat
    10321 gttgcagcga aaactgacga cgtaattgtg tttcatattc cagacagcct tcaaagcgag
    10381 aattaatctg cacgcgaatg atgccggact gatgcccttt ctccagtaat cgcgacactt
    10441 tcaaacgggt caggccgaga cgatcgctga tctcactctg ggtcagcccg tcgtggtagt
    10501 aaaaccacgc gatccgcgcg acctgttctt cttcacacat tccctgttct gaaattaccg
    10561 aatcgttgat tgtcataatt cattcttcac tttgaacata tttaaatctt taatgcaatt
    10621 gttcagttct tgcgcattta tatctgtgat ggcaaccaca gtttgactct acgagcatga
    10681 acaaacgcaa ccgtgaaaat caaaatagta taaattgtga tctattcgtc ggaaatatgt
    10741 gcaatgtcca cctaaggtta tgaacaaatt aaaagcagaa atacatttgt tcaaaactca
    10801 cctgcaaaac tgaacggggg aaatatgcaa acgagtgata cccgcgcgtt accgctactt
    10861 tgcgcccgct cggtttataa acagtattca ggggtcaatg tcctgaaagg catcgatttt
    10921 acgttgcatc agggggaggt ccacgccctg ctcggcggca atggtgccgg taaatcgacg
    10981 ttaatgaaga ttattgccgg tattacctct gctgatagcg gtacgctgga gattgggggc
    11041 aacaactacg ccagattaac gccagttcat gctcatcagc tgggtattta tctcgttccc
    11101 caggaaccgc tgcttttccc aagcctgtcg ataaaagaaa acatcctgtt tgggctggca
    11161 aaaaaacagc tctccatgca gaaaatgaag aacttgctgg cggcgctggg ctgccagttt
    11221 gatctgcata gtctggcagg atcgctggat gtcgccgatc gccaaatggt ggaaatcctc
    11281 cgcgggctga tgcgcgactc gcggattctg atcctcgatg aacctaccgc ctcgcttacc
    11341 cctgcggaaa ccgaacgctt gtttactcgc ttgcaagagc tgcttgctac tggcgtgggt
    11401 attgttttta tctcgcataa gctgccggaa attcgccaga ttgccgatcg gattagcgtg
    11461 atgcgcgacg gaaccatcgc cttaagcggc aaaaccagcg aactgtctac cgacgacatt
    11521 attcaggcca tcaccccagc ggtacgggaa aaatcgctct ctgccagcca aaaattatgg
    11581 ctggagttac ctggcaaccg cccacaacat gccgcaggaa cgtcggtgct gacactggaa
    11641 aatctgactg gcgaaggttt caggaatgtc agcctgacgc taaatgccgg agaaattctg
    11701 ggtctggctg ggctggtggg tgccggacgc acagaactgg ccgagacgct ctatggtcta
    11761 cgtactttgc gtggcggacg cattatgctg aatgataaag agatcaatag attatccact
    11821 ggagaacgtt tactgcgcgg tctggtttat ctgccggaag atcgccagtc atccggactg
    11881 aatctcgatg cttcgctggc ctggaacgtc tgcgccctta ctcataacct tcgtggattc
    11941 tgggcgaaaa ccgcgaaaga taatgccacc ctggaacttt atcgtcgggc gctgaatatt
    12001 aaatataacc aaccggaaca agctgcacgg acattatccg gtggtaacca gcaaaaaatc
    12061 ctgattgcca aatgcctgga agcctcgccg caagtattga ttgtcgatga gccgacgcgc
    12121 ggcgtggatg tctcggcgcg taatgatatc taccaactgt tgcgcagcat cgccgcacag
    12181 aatgtggctg tgctgcttat ctcctctgat ctggaagaga tcgaactgat ggcagatcgt
    12241 gtgtatgtga tgcatcaggg cgaaattacc cactctgcac tgaccgggcg cgatattaat
    12301 gtcgagacta ttatgcgcgt tgccttcggc gatagtcagc gtcaggaggc ggcatgctga
    12361 agtttattca gaacaaccgt gaaatcacgg cactgctggc ggtgctgctg ctgtttgtat
    12421 tacccggttt tctcgaccgc cagtatttaa gtgtgcaaac gctgaccatg gtttatagca
    12481 gcgcgcaaat cctgatcctg ctggcaatgg gcgcgacgct ggtaatgctt acgcgcaata
    12541 ttgatgtttc agtgggttcg attaccggaa tgtgcgcggt gctgttgggg atgttactga
    12601 acgcaggata ttcactacct gttgcctgtg tcgcgacttt actgcttggt ttgctcgcgg
    12661 gatttttcaa cggtgtcctg gtcgcgtggc taaagatccc tgccattgtt gccacccttg
    12721 gcacgttagg gttgtacaga ggcatcatgt tgctgtggac tggcggtaaa tggattgaag
    12781 ggttacccgc cgaactgaaa cagctctccg ccccgctgct gcttggcgtt tcagcaattg
    12841 gttggttaac gataattctg gtggcattta tggcctggct gctggcgaag acagcgtttg
    12901 gacgcagttt ttatgccacg ggcgataatt tacagggcgc tcgtcaactg ggcgttcgta
    12961 cggaagccat tcgcattgtg gcattttcgt tgaacggctg catggcggca ctggcgggaa
    13021 ttgtgtttgc ttcgcagatt ggttttatcc ccaaccagac cggtaccggg ctggagatga
    13081 aagcaattgc agcctgcgtg ctgggcggca ttagtttgct cggcggttcc ggtgcgatca
    13141 tcggtgcggt actcggcgca tggttcctga cgcagatcga tagcgtactg gtgctgttgc
    13201 gcattccggc atggtggaat gattttatcg cgggtctggt tctgctggcg gtgctggtgt
    13261 ttgatggacg cctgcgttgt gcgctggaac ttaatctgcg gcggcaaaaa tatgcccgct
    13321 ttatgacgcc accgccatcc gttaaacccg cttcgtcagg taaaaaacgg gaggctgcat
    13381 aatgcgtatt cgctacggtt gggaactggc tcttgccgca ctgcttgtta ttgagattgt
    13441 ctcatttggt gcaattaacc cgcggatgtt agatctcaat atgttgctgt tcagcaccag
    13501 tgactttatc tgcattggca ttgtcgccct accgctgacg atggtgattg tcagtggcgg
    13561 gatcgatatt tcgtttggtt cgaccatcgg cctctgcgcc attgcattgg gcgtactgtt
    13621 tcaaagtggt gtgccgatgc cgctggcgat tctcctgacc ttactgctcg gcgcattgtg
    13681 cgggctgatc aacgccggat taattatcta taccaaagtt aacccgctgg tgattacgct
    13741 tggcacgctg tatctgtttg ccggaagcgc cctgctgctt tccgggatgg ccggggcgac
    13801 ggggtacgaa ggtattggtg gattcccgat ggcgtttaca gatttcgcta acctggatgt
    13861 gttggggctc cccgttccgc tgattatctt cctgatatgt ctcctcgttt tctggctctg
    13921 gctgcataaa acccatgccg gacgtaacgt gtttttgatt gggcaaagcc cgcgcgtggc
    13981 gctttatagc gcgattccag ttaaccgtac cttatgtgcg ctctatgcca tgacggggct
    14041 ggcgtctgcg gtcgccgctg tgctgctggt gtcgtatttt ggttcagcac gttccgatct
    14101 cggtgcgtcg tttctgatgc ccgccatcac cgccgtggtg cttggcggtg ccaatattta
    14161 tggtggttcc ggtgccatta tcggcaccgc cattgcggtt ttattagtgg gatatttgca
    14221 acaaggtttg caaatggcag gagtgccaaa tcaggtgtcc agcgcccttt ccggtgcgct
    14281 acttatcgtc gttgtcgtag gtcgttccgt tagcctgcat cgccagcaaa ttaaagagtg
    14341 gctggcgcgt cgggccaata acccattgcc ataaaggata tcttcatgac acttcatcgc
    14401 tttaagaaaa tcgccttact tagcgctctt ggcattgccg caatctctat gaatgtgcag
    14461 gccgcagagc gtattgcatt tattcccaaa ctggttggcg tgggattttt taccagcggt
    14521 ggcaacggcg cacaacaagc gggtaaagag ctgggcgttg atgtgaccta cgacgggccg
    14581 acagaaccca gtgtttctgg tcaggtacag ttgattaata acttcgtcaa tcaaggttat
    14641 aacgccatta tcgtttctgc ggtttcgcct gatggcttgt gtccggcact gaaacgcgcc
    14701 atgcaacgtg gtgtgagagt gctgacctgg gactctgata ctaaaccgga gtgccgctct
    14761 tactacatta atcagggaac tcccgcccag ttgggaggta tgttggtgga tatggcggcg
    14821 cgtcaggtga ataaagacaa agccaaagtc gcgtttttct actcaagccc caccgttacg
    14881 gaccaaaacc agtgggtgaa agaagcgaaa gcgaaaatcg ccaaagagca tcctggctgg
    14941 gaaattgtca ctacgcagtt tggctataac gatgccacta aatcattaca aaccgcagaa
    15001 ggaatattaa aagcgtatag cgatctcgac gccattatcg cccccgatgc caatgccctg
    15061 cccgctgccg cacaagccgc agaaaacctg aaaaatgaca aagttgcgat tgtcggattc
    15121 agtacgccaa acgtgatgcg tccatatgtg gaacgcggca cggtgaaaga atttggcctg
    15181 tgggatgtgg ttcagcaagg caaaatttca gtttatgtcg cggatgcatt attgaaaaaa
    15241 ggatcaatga aaacgggcga caagctggat attcagggcg taggtcaggt ggaagtctcg
    15301 ccaaacagcg tccagggcta tgactacgaa gcggatggta atggcatcgt actgttaccg
    15361 gagcgcgtga tattcaacaa agagaatatc ggcaaatacg atttctgatg tgcattactt
    15421 aaccggagta agttatggca gatttagacg atattaaaga tggtaaagat tttcgtaccg
    15481 atcaaccgca aaaaaatatc ccttttaccc tgaaaggttg cggtgcgctg gattggggaa
    15541 tgcagtcacg cttatcgcgg atatttaatc cgaaaacggg taaaaccgtg atgctggctt
    15601 ttgaccatgg ttattttcag ggaccaacta ccggacttga acgcattgat ataaatatcg
    15661 ccccgctgtt tgaacatgcc gatgtattaa tgtgtacgcg cgggattttg cgcagcgtag
    15721 ttcccccggc gaccaataag ccggtggtac tgcgggcgtc aggtgcgaac tctatcctgg
    15781 cggaattaag caatgaagcc gtggcgttat cgatggatga cgccgtgcgc ctgaacagtt
    15841 gcgcggtggc ggcgcaggtt tatatcggca gcgaatatga acatcagtcg atcaaaaata
    15901 ttattcagct ggtcgatgcc ggaatgaaag tgggaatgcc gaccatggcc gtgactggcg
    15961 tgggcaaaga catggtgcgc gatcagcgtt atttctcgct cgcgactcga atcgccgctg
    16021 aaatggggac gcaaattatc aaaacctatt atgtcgaaaa aggttttgaa cggattgttg
    16081 ccggatgtcc ggtacccatt gttattgctg gcggtaaaaa attaccggag cgcgaggcgc
    16141 tggaaatgtg ctggcaggct atcgatcagg gcgcttctgg tgtggatatg gggcgtaata
    16201 ttttccagtc tgaccatccg gtggcgatga tgaaagccgt acaggcggtg gttcaccata
    16261 acgaaacggc tgatcgggca tatgaactct atctgagtga aaaacagtaa ctgcggattt
    16321 aaggagaaga attatgcacg tcacactggt tgaaattaac gttcatgaag acaaggttga
    16381 cgagtttatc gaagtttttc gccagaacca cctgggctct gtacaggaag aaggcaattt
    16441 gcgcttcgat gtcttacagg acccggaagt gaattcgcgc ttttatatct acgaagccta
    16501 taaagatgaa gacgcagtgg cgttccataa aaccacgccc cactacaaaa cctgtgtcgc
    16561 gaaactggaa tctttaatga ccgggccgcg taaaaaacgt ctgttcaatg ggttgatgcc
    16621 gtgaggcgaa tttatcagtt ttatctacaa ttggggtaac gcgctgacgg gagtaaataa
    16681 atgtctgact ggaacccctc tttatatctg cactttgccg ctgaacgatc gcgtccggcg
    16741 gtggagctgc ttgccagagt gccgctggag aatgtcgatt atgttgccga tcttggctgt
    16801 ggcccaggta acagcaccgc cctgctgcat caacgttggc ctgctgccag gataacgggc
    16861 atcgactcgt ctccggcgat gattgctgaa gcgcgcagtg ctttgccaga ctgccagttc
    16921 gtggaagcag acatccgcaa ctggcaaccg gaacaggcac tcgatctgat ttttgctaat
    16981 gcctcactgc aatggctgcc cgaccactac gaattgtttc ctcacttggt ttctttactt
    17041 aatccgcagg gcgtgctggc agtacagatg ccagataact ggctggagcc gacccatgtg
    17101 ctcatgcgcg aagttgcctg ggaacaaaac tacccggatc gcgggcgtga gtcgttggct
    17161 ggtgttcatg cttactacga tattttgagc gaagccggat gtgaggtcga tatctggcga
    17221 accacctact atcaccagat gccgtcgcac caggcgatta tcgattgggt gaccgccact
    17281 ggattacgtc cgtggttaca ggatctgacc gagagcgaac agcagctttt tcttacgcgc
    17341 taccatcaga tgctgaaaga gcagtatcca ctgcaagaga acggacagat actgctggca
    17401 tttccgcgtc tgtttattgt tgcccgccgt acggagtaaa tatcacgtca gttggtaatg
    17461 gcgatcggga agaacttttg ctggaatttc ggcttcatcg ttcatctgta agaggtcaat
    17521 ttcaatagcg ttacagatgg catccagcgg taaatcattg ttttcagtac cgaacggatc
    17581 ttccagttct tccgccagac aatccagcga aataaaagta taggaaatca gcacagagat
    17641 aaaaggcgtc atgtaatgca ggtccacgac cagcgcgaac ggcagcatga tacaaaacag
    17701 ataaacggta cgatgcaaaa tcagggtgta ggcaaaggga attggcgtgt aggcaatgcg
    17761 ctcgcagcct gccagaaccg ctgaaatatc gttaagccga tcgttgaggc taataaacag
    17821 gatatctgaa agttgtccat tgcgtcgctg aaccgccaac cactccccca ttattaacaa
    17881 gatgcggtta gctggagagt tcgaagccag tacacgctga agatcttcag tcttgagata
    17941 atgagccagc acttccacct gtggctgttt gcgtaatgtc atgcgtaaac agtgggcgaa
    18001 ggcgatttgc agccgggcaa actcccttac acttgccgaa tccggcaatg tcgtttttac
    18061 ctcgcgcagt aacgaccgtg aggcaatcat caactgcccc caaagtttgc gcgcttcaac
    18121 gtaacgggcg taaccggcat tattacgaaa accaagaaaa atggcgatgg cgacaccgag
    18181 aatgctgaac ggtgcgaggg tgaatttgat gcccagatgc gtgtaccagg gcagcatgaa
    18241 aataacagcg atagaaaaga gaaaattgag tagtaagcgc gaggatatct tggataatac
    18301 tgagccatgc cagacaaaaa tacggcgcag ccagtgttgt tgtggacgaa caatcatggt
    18361 tatcttcagg cgtggaaaag tcgccctatt aaacgtgatt acgatcacat tctcaagaca
    18421 ctacttacaa attacctaca aaattacaac attaacaggt gctgcatgtg gcacctgtta
    18481 atgataaagg cttataggat gttgtaacta atataatgaa ataagaaacc cggtcgaaac
    18541 cgggttcaga gtattagcac aacggacgta cagcttcgcg catccctttt tcgagaatcg
    18601 catccaggtc attagccacc tgctcgacca gaccaggcac ttgcgtcagg tcctgctccc
    18661 agtggtcttt ttctgccaac acaatcgcta ccagttcctg ggtaccgata acacggtcac
    18721 gatgctgact ccagagttgc tgataacgtt ccagccagtg tgcatcgtcc tgtaccggat
    18781 atgtttctcc attacgctca ccgcgataga acgcaatcaa tgccgctaat gcgaaagtaa
    18841 ggcgcgccgg aagtgtgccg tttgccttct gccctgccag cagctgcggc aggatgcggg
    18901 tacggaactt ggtcataccg ttgagcgcga tagacagcag ctgatgctta atgtacgggt
    18961 tacggaaacg cccggtgact gcactggcga aagattccag ttcatcgcgt ggcaaatcca
    19021 gtaccggaat aatttcttcg tagatggctt tttcaacgaa tgcgcaaatt tcagcatcgt
    19081 tcatcgcttc acctacggta tccagccctg cctggaacgc caccggcacc aacgcggtgt
    19141 gtgcaccgtt gagtattgcc actttgcgtt ctttatacgg tttaatatcg tcaacaatca
    19201 gcacgttgag cggatatttg tccagacgca gctcggtcgc taaggatttc ggtccctgaa
    19261 tcacaaacaa gtaaaagtgt tcagcggtgt cgaggaaacc atcgtgataa cccagttctt
    19321 cttccagttt agccacttca tcacgcggat aaccggtaac gatacggtcc accagcgtag
    19381 aacagaagct gttagcctga tccagccatt gaataaatgc ttctggcagt gcccactctt
    19441 gcgcatagcg cagcaccagt tcacgcaacg cgtcgccatt gtagtcgatc aactcacacg
    19501 ggatgatgat ccaaccttta tccagcgcac cgttgaaatg gctaaaacgt tcgaacagca
    19561 gacgggtcag ttttgccgga taacttaccg ctggcgcgtc atcgaattta tcgcccgcat
    19621 ggtagctgat acccgcttcg gtggtgttag agaaaacaaa gcgcatttcc gggttgtgcg
    19681 ccagtttcag gaattcatcg tattcactgt agacgctgat ttcacgatta acagagcgaa
    19741 tcagacgcgc gtcgctgacc gcttccccct tctcattcag gccacggata atggtggtgt
    19801 acagaccatc ctgcgtgctc agtgacggcg ggaatgaagt ttcaatcgga cgaacaacga
    19861 ccacaccaga attcagatcg gtgtgctcat tcaggagatc tatttgccag tcaacaaagg
    19921 cgcgcaggaa gttaccttca ccaaactgaa tgatacgttc tggatactgt gcaccgggaa
    19981 aatcgcgacg atttagtgtt ttcacaatgg gttcccttct gattagtcat acaacctgtt
    20041 tgaattggta cgacaggtta gcaaacttta atacgacgaa cccctgtttt gatcaactcc
    20101 tgatgattaa tgagcagttt tatgagaaaa gtgtggcgcg gatcatggtt taatcgagga
    20161 aatacgcctt ttcctggatc ataaagtggt agaacacatt gcattcaaat cgcgcggaat
    20221 gaataaagat gtcagacaac ttcctcaccg taacgcatag tgctggtacg gttgcgccca
    20281 tctttcttcg agcgatacag acaggtatcg gcttcgacca tcaatttatt aaagtcatcg
    20341 gtcagcgttc ggtacgatgc gcgaccacta ccgacgccaa tacttaccgt gagataaagc
    20401 gttttttgtt gccaggtgaa tggttgcagt tcaacgcctt tacggatttt ttccgccatt
    20461 agcagaccat cgacaggatt cactgacggc actgcaacag caaattcttc gccgcccatg
    20521 cgcgccacca gccccttatc accgacaatc ttctgaatat gccgggcaaa cacgcttaac
    20581 actttatcgc cacattcatg tccatagtta tcgttaatac ttttgaaata gtcgatatca
    20641 agtagcatga cagtcagatg ttgtgtctgt ttcagcgatg gacttttcag cgcctcataa
    20701 agaccggacc gtgagtaaac ctgagtcaga aaatcaaagt cggctcgcag cgcaacttgc
    20761 ttcattagcg aattgatcgc tgccacgcta aaagaaacca taattgggca tatcgccatc
    20821 gtggcaatac cgagacgtgc ggagaacatt tgcggaatgg agaacggcga accgaccgag
    20881 atatcaatca ccgaattagc caccagtacg atttccaccg caccggtgac aaaggtcaac
    20941 agacatgtta cctgcggcgt atagcgcact gcacaccaga ttaatgcagg cagcgggaac
    21001 gccagactcc ccgctccgcc aatgactacc gaggcaataa ccgacacaat aagcgcgatg
    21061 gcgggcatca tctgttctgc tttaaagcgc ggcagcactc caggaatagc caacgtcagc
    21121 atacaaggca cgatcaacac gcccgttgag aattgctcac tgaaccagtc cgcaagcaaa
    21181 ggccagaaat ccagactatc aatactgacc gaaccaatcg ccccgacaat agcgcataat
    21241 aatgcggcaa tcagacagta attaaatagc cgtaaggcac ttactggctc atatttattt
    21301 ttccccaaac gcttatcccg cacgaccagt aaggcgacgg taacaataaa catcatattg
    21361 gagaaattga tagccagtga aaccagtccc cattcggtgg ttatggcatc ataaaccagc
    21421 atcgccacat aactgatcgc atagtaatgc agtcgattaa gccagacata gcgggcaaac
    21481 acccccgcca ttacgccatt cagcggccag aaaagagaaa gttcgtgaac cagccgtaac
    21541 tctgcaccaa taaaatagaa cagcgtggtt agcgcaaaaa tagcgatgct attacgtagc
    21601 agatgacctt cctggaacac acggaaagtt gaaatggggt gtacatgcat taattatctg
    21661 tccatacaat tgcgggtggc aattatctca acgtaataag taaattctca taaaggatag
    21721 catgttcgct gacagcggcg gcaaataaag gtaccctggg aatgctattt tgtcgttttc
    21781 cgcaattctc atggctgatt catctgtaca accagccagt gatagattac cgccacgata
    21841 taacgttgtt gttcttcgct tagcgaaaca ccatgcggga tgttatgcca cttagccaga
    21901 caaccgcgac agcaggtcgc ggtggcgtgc tgagcgataa acaccggatg cccgcgcatg
    21961 ggggtttgct taccgtcatt agccggtaat gccggagcaa gacgtttagc gacaaaatcc
    22021 gccgcatgtt gttcgatgac tggcgcgcct ttctccagac aatactgacg ctctttcatg
    22081 cccaaacgaa agcgagatcg aaattttgaa cgggataaac gcgcgaagag tggatcgaga
    22141 gactgcatta ataaaccgaa cgccctaact gtttcgtcaa ttgttcaagg acggcgatac
    22201 ccgcaagcga gttacctgca tcatccagtt ccggactcca gactgcgatg gccatttcat
    22261 gcggaacaat cgccacaata ccgccaccaa cgccagattt cgccggtagt cccacccgcc
    22321 aggcaaactc ccccgcgttc tggtacatac cactggtcgc catcagcgcg ttaatttgcc
    22381 gtgcctgcat tggcgtcacc acaggttcat caatatgaat agctttcccc tgattagcca
    22441 gaaagacaaa cgtccgggcc agctctacac agctcatttt cagagcgcag taatgaaaat
    22501 agttttgcag aacggttgtc acgtcatgat ggaaattgcc aaacgacttc atcagccagg
    22561 cgatagccgc atttcgcgcg gaatgttcaa attcggaacg cgctaccacc gtatcgtagg
    22621 aaatatcaga cacaccgctt aagccgcgca cgacttccag catacgttgc cgtggtgcgc
    22681 ttaatcgccc ttgcaacata tcgcagacca ccagcgcacc agcattaata aacggattac
    22741 gcggaatacc ctgctccatt tccagttgca ctaaagaatt gaacggtgat ccagacggat
    22801 ctttgccgac gcgttgccag atttcctctt cggagtaatg acgcatggcg acaacgagac
    22861 tcagcacttt ggaaatagac tgaatggaaa aacgttcttg cgcgtctccg gcctgaaaaa
    22921 gctgtccgtc aacggtacag atagcaatgc ccaatcggga accgtctact gtagccagcg
    22981 ccggaatata atccgcgact ttaccctgac caatgagcgg ccgcacttgc cgcaagatgt
    23041 tttctaaaat tgcattatcc atggcgactg ccactttcta ctcctggacc gcaggtctga
    23101 aaagacctgc gagtatatca gagctgaata tgtcgcgtca gatccggtct ttccacaccg
    23161 tctggatatt acagaattcg tgtaagccga aatgggaaag ctcacgacca aagccactct
    23221 ttttcacgcc accaaaggcc actcgcgcgt cgctggcaca ataaccattg ataaacaccc
    23281 caccgcattc cagacgtgcc gccatctgtc tggcctgtgt ttcgtcggtg gtaaaaatgg
    23341 tcgctgaaag gccgaactca ctatcattag ccagttccag cgcatgttct gcatctttcg
    23401 caatggtgat tgccgcaacg gggccaaaca tttcttcccg aaaagcggtc atttctgggg
    23461 taacattcgc cagaaccgtt ggcggatagt agttacctgc cccagccatc ttttccccgc
    23521 ccagtaacaa acgcgcaccc tgcgccaggg ttttctccac ctgatgatgc agctcatcac
    23581 gtaaatcgaa acgagccatt ggtccgagag cgttctcttc gtcacgggga tcgtccattt
    23641 tcaaggctgc cgcagctgcc acaaaacgtt cggtaaatgc cgaagcaatt ccctcttcga
    23701 taataaagcg ttttgctgct gcacaaacct gtccggtatt ctgataacgt ccggctaccg
    23761 ccgctttaac tgccagttcc agatcggcat cgttaagcac aataaatgga tccgaaccgc
    23821 ccagttccag tacgcatttt ttcagtgccg ctccagcctg tgcaccaata gccgctccag
    23881 cacgaacact tccggtcacc gtgatagcag caatgcgcga gtctttaatc atctgagtga
    23941 caccgtcatt gtcggcattc agccagccat atacgccttg tgggattccc gcatctttaa
    24001 acacctgggc aatgagctga gcacagccca tcacattcgg cgcatgttta agtaagtatc
    24061 cgttaccagc aagaatgatg ggaacagcgc cacgcatcac ctgccataac ggaaaattcc
    24121 acggcataat cgccagaatc gtccccaacg gtcgatactc aataactgcc tgctgatttt
    24181 ccaccagcgt aggttccgcc ttcagcattg ccggaccatg ttctgcatac cagtcacaca
    24241 aattcgccga tttcgccact tcagcgcgcg cctggttgat tggtttgccc atttcgcggg
    24301 tgatcatttg cgccatttct tcgctacggg cgcgcagagc cttaccgata tcacgcagtt
    24361 tttcagcacg ataatctata tttgtctcgc gccagtcacg aaagcctgct gccgccagct
    24421 gaagtgcgtt ttcgatatca tcagcgccag cccacggcaa cacagaaagt tgttcacccg
    24481 tggcaggatt tatcgaaatt gcatgagttg ccggagtaat ggtcatcggg gtatctcctt
    24541 tatgagtcat ggtatgaaga tacgcagatt tactcttgct ttaaaatgaa taatattaag
    24601 ccacttattc acgaatcgag aatattatgg atctgaccca actggagatg ttcaacgcgg
    24661 ttgccgaagc tggcagcatt acgcaggctg cagcaatagt gcatcgcgtg ccgtcgaatc
    24721 tcactacccg tctacgccag ctggaaacag aactgggggt agatctgttt attcgcgaga
    24781 atcagcgttt acgtctatct cctgccgggc ataacttttt acgctatagc caacaaattc
    24841 tcgcgttagt ggatgaagcg cggagcgttg tcgctggcga tgagccgcaa ggtttatttt
    24901 ctctgggttc gctggaaagc accgcagcag ttcgcattcc ggccacgctg gcggaattca
    24961 accgtcgtta tcccaaaatt cagttttcac tttccaccgg cccttccggc actatgctgg
    25021 aaggtgtgct ggagggaaaa cttaatgcgg cgtttattga tggacccatt aaccatactg
    25081 ccattgacgg gataccggta taccgcgagg agctgatgat cgtcacgcca caaggtcatg
    25141 cgccagtaat ccgtgccagt caggttaatg gcagtaacat ttatgccttc cgcgccaatt
    25201 gttcgtatcg tcgccacttc gagagctggt ttcatgctga cggtgccgct ccgggaacta
    25261 tccatgagat ggagtcttat cacggaatgt tggcctgtgt ggtcgcagga gcaggtattg
    25321 cgcttattcc gcgctctatg ctggaaagta tgccggggca tcaccaggtt gaagcgtggc
    25381 cgttagctga gcaatggtgt tggctaacaa cctggctggt ctggcgtcgt ggtgcgaaaa
    25441 cgcgtccgct cgaggcattt attcaactac tggatgtgcc tgactcgaca aaacagggtt
    25501 accaatgagc tatttttgat agttcttgcg gctaatatgc tctatatagt gatgttccga
    25561 tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc
    25621 tcttcctgat gattttcccg ttcgtaatat tgcaccgcag gtaaaagaag ttttaaagga
    25681 ttttattgat gcacttagta caataatatg taatgaagag tggcgcacgt ctttaaacat
    25741 caactcagcc acaaaaaaga tatttaataa tcttgacaac ttatcatata ttcagaggac
    25801 ttcttttcgg ggtaacgaca cgctatacaa tgaaaaggtt cagtttaaac tcacttatcc
    25861 cgccaggaat ggaagacaca aagaaaatat tgaatttcag gtagtaataa atttaagccc
    25921 tatttattta gataatttcc gccatgatgg agaaattaat attttttgcg ctcccaaccc
    25981 aaagcctgtt actatggggc gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt
    26041 tctgaatgat tttattgaac aattcccaat gatcaaccct ggcgttccca tcaaaagagc
    26101 gcatacacca catattgaac ccctgcctcc ggatcaccat accgctgcag attacttacg
    26161 ccagtttgat ttgcttgtcc tgaattttat ctctcgcggt aattttgtca tactcccccg
    26221 attatggaat aactctgagg ttcacagatg gttcgtcaat aaagatccta acttgatcac
    26281 cgccattctc gacataacgg acagcgaatt aaaagaggat ttattgcaaa gcctgatgga
    26341 ttcattaggt tctaacaaac atgtactacc ctaagtctgc atctgttttt tatccctttt
    26401 agcagaacaa gaatctcctc attttcagga cttgttttta tttttcgcca atatgttgtt
    26461 acactatcac caatttatga atcccaatga aagtgatttg aatgacgtgt tgatgccagc
    26521 atcattaagt gatgataaaa ttatcaaaca tatggcgcgt aagaccctca aactgtttgt
    26581 aaaaaatgaa acaccgccaa aagttactca tgaagatctg gtgaaaaaca ggcctcgctc
    26641 ccctgtcaga ccgcctatac ccgcaacctc caaaacgcca gacattcctg atcgtcatta
    26701 aaccgtgatg tttaccatct ctctgacgcg tgaaagaatc agcgtcagag aaacggaaaa
    26761 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
    26821 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
    26881 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
    26941 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
    27001 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
    27061 aatgaccagc caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
    27121 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
    27181 tgtggctttt gcacatgcga ttttctggtc gattacggcg tccctggcga tccgtatggc
    27241 tccggccggg aagcgagcac aggcattgag tttaattgcc acaggtacag cactggcaat
    27301 ggtattagga ttacctctcg ggcgcattgt ggggcagtat ttcggttggc gaatgacctt
    27361 cttcgcgatt ggtatcgggg cgcttattac ccttttgtgc ctgattaagt tacttccctt
    27421 actgcccagt gagcattccg gttcattgaa aagcctcccg ctattattcc gccgcccggc
    27481 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
    27541 ctatattgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
    27601 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
    27661 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gcctggcact
    27721 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
    27781 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
    27841 caccgacgtc gcgatggcgc tattctccgg gatatttaat attggaatcg gggcgggtgc
    27901 gttggtaggt aatcaggtga gtctgcactt gtcaatgtcg atgattggtt atgtgggcac
    27961 ggtgcctgct tttgccgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
    28021 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tgattcgttt
    28081 taatgatttc caggatgccg ttaataataa actgtacacc catacatacc agcaggaacc
    28141 ccatcaagcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccag
    28201 agctgcgtag acttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
    28261 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
    28321 tcatcgcaat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaagcaatat
    28381 tggcgctggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
    28441 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
    28501 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgcccg gcgtaatacg
    28561 ccaccatcat gatggcaaag acgtacaccg aggccatcaa cgactgacga ttacgttcgg
    28621 caccgttcat gttgcctgcc aggccaagaa ataacgcgac agttgttaat gggttagcta
    28681 acggcagtaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
    28741 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
    28801 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
    28861 gcatcggtca attcattcat ttgacttata cttgcctggg caatattatc ccctgcaact
    28921 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
    28981 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
    29041 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
    29101 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
    29161 gtatgctgga tcgcctggtc tgtaaaggct gggtagaaag gttgccgaac ccgaatgata
    29221 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgtgaa caatgccatc
    29281 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagtgg
    29341 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
    29401 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
    29461 ctggaatcac cactgtcact ggagaaagtg tcagagcgtt cgggttactc taaatggcac
    29521 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
    29581 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
    29641 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
    29701 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
    29761 ttaaaccatt acaacagcta gttgaaaacg tgacaacgtc actgaggcaa ttatgaaacc
    29821 gcttttatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
    29881 aacccagcaa ccgctcgtta cttcctgtgg cgatgtggtg gttgttcccc catcgcagga
    29941 acaaccaccg ttcgatttaa atcacatggg tacaggcagt gacaaatcgg atgcgctggg
    30001 cgtgccctat tataaccaac aagccatgta gtatattcag gccccggcat atcggggctt
    30061 gttaacctct taccttcacc gctttacgcc actgcaaacc aaatacattg atatacagtc
    30121 cggccataat gagcacagcc cctaagaatt gcagacctgt taagcgttca tccagcagta
    30181 acgctgcact tgccagaccg actaccggca ccagtaatga taatggtgcg acacgccatg
    30241 tttcatagcg tccaagcagc gtaccccaaa tcccgtagcc aactatcgtc gctacaaatg
    30301 ccagatacat caacgccaga atggtggtca tgtcgatagt gaccagactg tgaatcattt
    30361 ttccggagcc atcgagaatg agcgaggcga caaaaaacgg aattatcggg attaatgcgc
    30421 tccagacgac cagagacatt accgccggac gggatgaatg agacatgatc tttttgttga
    30481 agatattgcc actcgcccaa ctaaatgccg ctgccagagt caacataaag ccgagcatcg
    30541 ccacatgctg accgttaaag ctatcttcga ttaacaccag tacgccagaa atcgctaacg
    30601 caatccccgc cagttgctta ccgtgcagcc gttcgccaaa agtaaacgcg ccaagcacga
    30661 tagtaaaaaa cgcctgagcc tgtaagacca gcgaggccag tccagccggc ataccgaagt
    30721 taatggcgca gaagagaaag gcaaactgtg caaaactgat ggttaaacca taccccagca
    30781 gtaaattcag cggtactttt ggtcgtgcca cgaaaaagat tgccgggaag gcgaccagca
    30841 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    30901 caaaattaag cccccataca acgacgacta gtagcgccaa caccccatct tttcgcgaca
    30961 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    31021 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    31081 acttcggatt tttgccatgc tatttcttta catctctaaa acaaaacata acgaaacgca
    31141 ctgccggaca gacaaatgaa cttatcccta cgacgctcta ccagcgccct tcttgcctcg
    31201 tcgttgttat taaccatcgg acgcggcgcc acgctgccat ttatgaccat ttacttgagt
    31261 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    31321 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    31381 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    31441 gtgacgctgg tggtgctctt ttttgccctc attaactgcg cctattccgt ttttgctacc
    31501 gtgctgaaag cctggtttgc cgacaatctt tcatccacca gcaaaacgaa gatcttctcg
    31561 atcaactaca ccatgctcaa tattggctgg accgtcggcc cgccactcgg cacgctgttg
    31621 gtcatgcaga gcatcaatct gcccttttgg ctggccgcta tctgctctgc gtttcccatg
    31681 tttttcattc aaatttgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    31741 gtctggtcgc cgaaagtttt attacaagat aaggcactgt tgtggtttac ctgctctggc
    31801 tttctggctt cttttgttag tggcgcattt gcttcatgca tttcacaata tgtgatggtg
    31861 attgctgatg gtgattttgc cgaaaaggtg gtcgcggttg ttcttccagt gaatgctgcc
    31921 atggtggtta cgttgcaata ttccgtgggc cgccgcctta acccggctaa catccgcgcg
    31981 ctgatgacag caggcaccct ctgtttcgtc atcggtctga tcggttttat tttttccggc
    32041 aacagcctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aataatttat
    32101 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    32161 ttttccgccc agtcattagg ctggttgggc gcagcggtaa atccattagt gagtggtgtt
    32221 gtacttacca ctctcccgtc gtcttcgctg tttatcattc tggcgttagt catcattgcc
    32281 gcgtgggcgc tgatgttaaa aggtattaac gccagacccc agaggcaacc agcactctgt
    32341 tgacgttcgt gccgttccac tactggcacg gcttcgctaa tcattattct gaggcaaagg
    32401 catagccttc atcttcccag ccggttatcc cgccgagcat gattttaacc ggctttccga
    32461 gtcgagccag tttgagtgcc gcacggtctg caccgttaca atgtggcccc gcgcaataca
    32521 ccacaaatag cgtatcgtcg ggccattcgg ccattctttc tgcggtcatt tgcttatggg
    32581 gtaaatggag agcgcctggg atatgccgac gcgcgaagtc ctcttcacgt ccaaccacat
    32641 gaagtaaaac gaaatcatcg ccaatataag ccagagagtc atgaacatcg gcgcaatctg
    32701 tttcgacact caagcgcgcg agaaaatgcg cctcagcaac ttctggcttc gctgtggtaa
    32761 attcagtgac ataacgcata ttgctttgtc cttttggttt gttgtgttaa caataatgta
    32821 actaatctac ctttcactga ccgcctctcg atatgccaga aatgagcaaa atgatgacaa
    32881 cgttacaacg tccgctggtc attgcactgg catatgacgg gctatgtact tttgaatttg
    32941 gcgttgccgt tgaaatattc ggcctgccac gaccggaact gggcgataac tggtatcgtt
    33001 ttgctgttgc tgcggtagaa ccaggtgcgc tacaagccac cggggggatc cacgtggttg
    33061 ctgatggtgg actggagtta ttgcccaacg ccgatatgat tattattcct ggctggcgcg
    33121 gaatagatgc caaagtgccg gagatgctat gccagtcatt gcgcgaggca caccagcgcg
    33181 gtactcgcct ggtatcaata tgctctggcg tgtttgtcct tgctgctgcg ggccttcttc
    33241 atcagcgaaa agcaaccacc cactggcgct acactgaaca actccagcac cgttaccctt
    33301 caatcagcgt aagtgacaat gtgttatatc aggatgaagg acaagtgatg acctccgccg
    33361 gaagcgccgc ggggatcgat ctgtgtttac atatcgtgcg caaagatttt ggtcatgaga
    33421 ttgctaataa tgtcgcccgg cgactggtca ttcagccaca tcgccagggg gatcaaccac
    33481 aaaatctgac gcgcccaatg gcctcaccgc gtgaaagcca gacgttaggt gccttattcg
    33541 actttttgca gcagaatctg gcacaaacac atactgtgtc ttccctcgcc gaacgggtaa
    33601 atatgagtcc gcgcactttt ttaagacgtt ttgaggcgtt aactggcatg acgcccgccc
    33661 gctggctgct caatgagcgc ttaatacgag tacaggatta tctgcaaaat tcggttttta
    33721 acatcgagaa aattgccgag ctgacgggtt ttggcaattc agcgttattg cgtcatcact
    33781 ttcaccaacg ttttttatta tctccttcgc aatatcgcaa aaacagccgt tccccatcac
    33841 aataatcgcc tgattcagcc tttgtttaga taacctctgc taatatcttt ctcattgaga
    33901 taaaaattaa ggtaagcgag gaaacacacc acaccataaa cggaggcaaa taatgctggg
    33961 taatatgaat gtttttatgg ccatactggg aataatttta ttttctggtt ttctggccgc
    34021 gtatttcagc cacaaatggg atgactaatg aacggagata atccctcacc taaccggccc
    34081 cttgttacag ttgtgtacaa ggggcctgat ttttatgacg gcgaaaaaaa accgccagta
    34141 aaccggcggt gaatgcttgc atggatagat ttgtgttttg cttttacgct aacaggcatt
    34201 ttcctgcact gataacggat cgttgacaca gtagcatcag ttttctcaat gaatgttaaa
    34261 cggagcttaa actcggttaa tcacattttg ttcgtcaata aacatgcagc gatttcttcc
    34321 ggtttgctta ccctcataca ttgcccggtc tgctcttcca atgaccacat ccagagtctc
    34381 ttcaggaaat gcgcgactca cacctgctgt cacggtaatg ttgatatgcc cttcagaatg
    34441 tgtgatggca tggttatcga ctaactggca aattctgaca cctgcacgac atgcttcttc
    34501 atcattagcc gctttgacaa taatgataaa ttcttcgccc ccgtagcgat aaaccgtttc
    34561 gtaatcacgc gtccaactgg ctaagtaagt tgccagggtg cgtaatacta catcgccgat
    34621 taaatgcccg taggtatcat taaccaattt aaatcggtca atatccaaca acattaaata
    34681 aagattcaga ggctcagtgt tgcgtaactg atgatcaaag gattcatcaa gaacccgacg
    34741 ccccggcaat cccgtcaaaa catccatatt gctacggatc gtcagcaaat aaattttgta
    34801 atcggttaat gccgcagtaa aagaaagcac cccctcctga aaggcgtcga aatgcgcgtc
    34861 ctgccagtga ttttcaacaa tagccagcat taattcccga ccacaattat gcatatgttg
    34921 atgggcagaa tccattagcc gaacgtaagg taattcatcg ttatcgagtg gcctcagatg
    34981 atcaatccac cgaccaaact ggcacagtcc ataagaatgg ttatccgtta tttctggctt
    35041 actggcatct ctcgcgacca cgctgtgaaa catactcacc agccactgat agtgggcatc
    35101 gatagcctta ttgagattta acaagatggc atcaatttcc gttgtcttct tgatcattgc
    35161 cactcctttt tcacagttcc ttgtgcgcgc tattctaacg agagaaaagc aaaattacgt
    35221 caatattttc atagaaatcc gaagttatga ttcatctctg agataacatt gtgatttaaa
    35281 acaaaatcag cggataaaaa agtgtttaat tctgtaaatt acctcttcat tatcgcaaat
    35341 gaaaggatga caaatagcat aacccagtac ccaaatggcc cggtagttca ggccatcagg
    35401 ctaatttatt tttatttctg caaatgagtg acccgaacga cggccggcgc gcttttctta
    35461 tccagactgc cgctaatgtt gatcatctgg tccggctgaa cttctcgtcc atcaaaaacg
    35521 gtcgcaggaa taacaacatt aatttcacca ctcttatcgc gaaaaacgta acggtcctct
    35581 cctttgtgag aaatcaaatt accgcgtagt gaaaccgaag cgccatcgtg catggttttt
    35641 gcgaaatcaa cagtcatttt ttttgcatca tcggttccgc gatagccatc ttctattgca
    35701 tgaggcggcg gtggcgctgc atcctgtttt aaaccgccct ggtcatctgc caacgcataa
    35761 ggtatgacaa gaaaacttgc taatacaatg gcctgaaatt tcatactaac tccttaattg
    35821 cgtttggttt gacttattaa gtctggttgc tatttttata attgccaaat aagaatactg
    35881 ccaattgtta taaggcattt aaaatcagcc aactagctgt caaatattca gagaatttaa
    35941 ctcactaaag ttaagaagat tgaagagtct taaacatatt ttcagaataa tcggatttat
    36001 atgtttgaaa attattatat tggacgagca tacagaaaaa gcaaatcacc tttacatata
    36061 aaagcgtgga caaaaaacag tgaacattaa tagaaataaa attgtacaac ttgcagatac
    36121 cgacactatt gaaaacctga catccgcgtt gagtcaaaga cttatcgcgg atcaattacg
    36181 cttaactacc gccgaatcat gcaccggcgg taagttggct agcgccctgt gtgcagctga
    36241 agatacaccc aaattttacg gtgcaggctt tgttactttc accgatcagg caaagatgaa
    36301 aatcctcagc gtaagccagc aatctcttga acgatattct gcggtgagtg agaaagtggc
    36361 agcagaaatg gcaaccggtg ccatagagcg tgcggatgct gatgtcagta ttgccattac
    36421 cggctacggc ggaccggagg gcggtgaaga tggtacgcca gcgggtaccg tctggttcgc
    36481 gtggcatatt aaaggccaga actacactgc ggttatgcat tttgctggcg actgcgaaac
    36541 ggtattagct ttagcggtga ggtttgccct cgcccagctg ctgcagttac tgctataacc
    36601 aggctggcct ggcgatatct ccaggacagc cattggtggt gtttatatgt tcaagccacg
    36661 atgttgcagc atcggcataa tctgaggagc cttaccgcgc cattgtcgat acaggcgttc
    36721 cagatcttcg ctgttacctc tggaaaggat cgcctcgcga aaacgcagcc cattttcacg
    36781 cgttaatccg ccctgctcaa caaaccactg gtaaccatca tcggccaaca tttgcgtcca
    36841 cagataagcg taataacctg cggcatatcc gccaccaaaa atatgggcaa aataactgct
    36901 gcgatagcgt ggcggtatag caggaagatc catattttct gccaccagcg cccgcaattc
    36961 aaaatcatcg acatcctgca ttgcttcgtt ttcttccagg caatgccagc gcatatcgag
    37021 aagtgcggcg ctaagcagtt cgctcatctc ataccctttg ttgaacaggc tggcattacg
    37081 cattttctgt tgcagttcgt caggcattgc tgccccgctc tgataatgcc gggcgtagcg
    37141 agcgaatacc tgcggatgcg ttgcccagtg ttcgttgatt tgcgacggaa attcgacaaa
    37201 atcacgcggc gtgttggtgc cggagagcgt ggcataacgc tggcgggcaa aaaggccgtg
    37261 cagcgtatga ccaaattcat ggaataaggt tatgacatca tcccagagta acaacgcagg
    37321 ctcaccggca gcgggtttct gataattgca gacgttataa attaccggat gcgtttcatt
    37381 aagcgttgat tgctcaacaa aattgcccat ccatgcaccg ccgctttttg aatcacgggc
    37441 gaagaaatca ccgtaaaata acgccagccc cacgccatta tgatcaaaaa tttcccacac
    37501 ccgaacgtca ggatggtaga caggaatatc aaaacgttcg acaaacttaa taccgaagag
    37561 ctgattcgcg gtccagaata caccttcatt caacaccgtg tttaattcaa aatatggctt
    37621 gagctgcgcc tcatcaagat caaatttctc tcgccggacc tgttcggcat aaaatgccca
    37681 gtcccacggc tgcgcgctaa acccgccctg ctgcttatca ataaccgcct gtatggaggc
    37741 taattcatcg ctcgcacgtt gacgcgccgc tggaacaatt tcccgcataa agttgagtgc
    37801 tgcttcaggt gtttttgcca tctgatcggc gattttccat gcggcataat gaggaaaacc
    37861 gagtaatgta gcctgttgtg cacggatctc caccagacgt tgaatgatag cgcgggtatc
    37921 attggcatca tttttttccg ctcgcgtcca gcccgcaata aacagttttt cacgcgtcgc
    37981 acgatcgcgc atttcggcaa gcgccggttg ttgggtggta ttcagcagcg gaatcagcca
    38041 tttgttatcc agacctttct cgcgagccgc ctctgccgcc agcgcaatct cttgctcact
    38101 cattcctgcc agctgcgcga tatcgttcac aaccagaccg ccggatttat ttgctgccag
    38161 taaccgctgg ttaaactgac tggtcagtgt cgcagcttct gtattcagta cttttaattt
    38221 tgctttatca gcttgtgcaa gtttggctcc ggctaggaca aaacgttgat gaatcacctc
    38281 caccaggcgg atggattcac tatcaagccc cagagattca cggcgttgcc agacagcatc
    38341 tacccgcgcg aataattcac cgttcagata gatatcatta gccagttccg ccagttcagc
    38401 ggaaaactgc tcgtcaagac gctgtaattc atcattggta tgcgccgcag tcatcgcaaa
    38461 aaagacgctg gtaacgcggg taagtaattc tccgctttgt tccagtgcca gaatagtatt
    38521 gttgaaatca ggtgtttgcg ggttaagcgc gatggcagca atttctgccc gcttttgctg
    38581 tattccctca tcgaatgccg ggcgatagtg atgattggca atttgatcaa aatgaggagc
    38641 cagatacggc agtgtgcttt gcacaaggaa aggattaatt gttgtcattt tcttctcctg
    38701 aacgcgaggt gttccatagc gtaggcttac tgataacgga gtgcaatctt gcaaaccagt
    38761 attacccgct cttaagcatc acgtgctatg ttattgacac acaaaagcgt tgaggaacag
    38821 tgagatgatc gttttagtaa ctggagcaac ggcaggtttt ggtgaatgca ttactcgtcg
    38881 ttttattcaa caagggcata aagttatcgc cactggccgt cgccaggaac ggttgcagga
    38941 gttaaaagac gaactgggag ataatctgta catcgcccaa ctggacgttc gcaaccgcgc
    39001 cgctattgaa gagatgctgg catcgcttcc tgccgagtgg tgcaatattg atatcctggt
    39061 aaataatgcc ggtctggcgt tgggcatgga gcctgcgcat aaagccagca ttgaagactg
    39121 ggaaacgatg attgatacca acaacaaagg cctggtatat atgacgcgcg ccgtcttacc
    39181 gggtatggtt gaacgtaatc atggtcatat tattaacatt ggctcaacgg caggtagctg
    39241 gccgtatgcc ggtggtaacg tttacggtgc gacgaaagcg tttgttcgtc agtttagcct
    39301 gaatctgcgt acggacctgc atggtacggc ggtgcgcgtc accgacatcg aaccgggtct
    39361 ggtgggtggc accgagtttt ccaatgtccg ctttaaaggc gatgacggta aagcggaaaa
    39421 aacctatcaa aataccgttg cattaacgcc agaagatgtc agcgaagccg tctggtgggt
    39481 gtcaacgctg cctgctcacg tcaatatcaa taccctggaa atgatgccgg ttacccaaag
    39541 ctatgccggg ctgaatgtcc accgtcagta atttttatac ccggtgtaac tgccgggtta
    39601 ttgcttgtca caaaaaagtg gtagactcat gcagttaact cactcacaag caagaacgaa
    39661 tgaccgtcga aacgcaactt aatcccacac agcctgtcaa tcagcagatt tatcgtattc
    39721 ttcgtcgcga cattgtccat tgcctgattg caccaggcac accgttgtcg gaaaaagaag
    39781 tttctgttcg tttcaatgtg tcacgccagc cggttcgtga agcctttatt aaactggcgg
    39841 aaaacggcct gattcaaatt cgtccgcagc gtggcagcta cgtcaacaaa atttccatgg
    39901 cccaggtgcg caacggcagt tttatccgtc aggccattga gtgcgcggtg gcgcgtcggg
    39961 cggcgagtat gattaccgaa agccagtgct accaactgga acaaaatctt caccagcaac
    40021 gcattgccat tgagcgcaag caactggatg atttttttga acttgatgac aacttccatc
    40081 aactcctgac gcagattgcc gactgtcaac tggcgtggga taccattgag aacctgaaag
    40141 cgaccgttga tcgcgtgcgc tatatgagtt tcgaccatgt ttctccacca gaaatgctgt
    40201 tacgccagca tcttgatata ttctctgccc tgcaaaaacg tgatggcgat gcggtagaac
    40261 gtgcaatgac gcaacatttg caggaaatca gcgaatccgt gcgccagatc cgccaggaaa
    40321 atagcgactg gtttagcgaa gagtaattca tttcctctca tcccatccgg ggtgagagtc
    40381 ttttcccccg ccttatggct catgcatgca tcaaaaaaga tgtgagcttg atcaaaaaca
    40441 aaaaatattt cactcgacag gagtatttat attgcgcccg ttacgtgggc ttcgactgta
    40501 aatcagaaag gagaaaacac ctatgacgac ctacgatcgt aaccgtaacg caatcaccac
    40561 tggcagccgt gttatggtta gcggcaccgg tcacactggc aagatcctgt cgattgatac
    40621 tgaaggtctg accgctgagc aaatccgccg cggaaaaacc gtagttgttg aaggttgtga
    40681 agagaaactg gcaccactgg acctgattcg tctcggcatg aactaagcgt gtgaatgccg
    40741 ccgatggcgg cattgctttt tttacttcac ggaatatttc gccacggtcg ctttcgcacc
    40801 atgcgctaat aaagacaagt acgcttccgt cacttttgca gtaaacaagc tattgtctgg
    40861 caaatcacca ccaaaaatcg ccttaatcgc cagcaatgac tggacgcgcg ctttcccttc
    40921 ggcactactt tgtacagcct tctgaataac aggtaacagt gggtcactga tttctatcgg
    40981 atttccctgt tcatcaacac caccgacata acgcatccaa cccgcgacgc ccagcgccag
    41041 cagatcgaac ttgctgtcat gcgccagatg ccagcgaaca gaatccaaca tccgctgtgg
    41101 caatttctgg ctgccatcca tcgcaatctg ccaggttcga tgacgtaacg ccgggttgct
    41161 atagcgtgca attaatcggt tagcgtaatc ttgcaaatca acgccctgca ctttcagcgt
    41221 cggcgcttgt tcctgcaaca tcaaggcata cgccgcatga cgataatgtt catcttccat
    41281 acagtcatta atgtgctgat atccggcaag ataacccaga tacgccagga atgaatgact
    41341 gccgttgagc atgcgcaact tcatctcttc ataaggcagc acatcactaa ccagttcggc
    41401 tcccgctttt tcccattccg gacgtccggc aacaaagtta tcttcgatta cccactggcg
    41461 gaaaggttca caggcaacgc cagcaggatc gcgcacaccg gtaagctgtt cgattttcgc
    41521 cagcgtatcc tctgtcactg cgggcacaat acggtccacc attgttgatg ggaaagtcac
    41581 gttatcttcg atccattgtg ctagttttac atcaacggct tgtgcgtagg aagtgacaac
    41641 gtcacgcata acatgaccgt tttctggcat gttgtcacat gacatgacgg taaatgcggg
    41701 aagtcctgcc gctttacggc gagccagcgc ctcaacaatc acccctgttg ctgttttcgg
    41761 ctggtgggga ttttgcacgt cggcagcgac catcgggtga tcgagcatta actgtccggt
    41821 cgccggagag tggaaatacc ctttttcggt gattgtcaga gagacaatcg cgatttgcgg
    41881 ttcacacatc gcagccaaca cggtttctaa gccatctatc tgtacgtgca aggctttttt
    41941 aacgacgcca acgacgcgag ccgtccacgc atcggccgac atttccgcaa cggtataaag
    42001 attatcttgc tgatgtaaat cggcaatttg ctgttcgccg ccgattaagt tgacctcata
    42061 atatccccag tcactgaaat gttctgtagc aagaatatcg gcatacacgc cctgatgcgc
    42121 acggtgaaat gcaccaaagc ctaaatgaac aattcttggg gccaggttat tacgatcata
    42181 aacagggagt gtcgcttttg ctgataacaa attatttccc ataacaattc cttaaattta
    42241 aatatggcaa gctacatgtt ttgttatatg aataaaaatc ccctctccgg taagagaagg
    42301 gattgaggat ttacagactt ctggaaggtt gcgcagctct tacaacacgc ggttgatctt
    42361 ccgcagcgtc ttccagcgca cttaaatcac ggtctttcac ttctggcatt ttcagcgcag
    42421 agattaaacc aatcactgaa tatgccatga tcataatggc gatcggatac caggattccg
    42481 tcatggtgca gaaaataccc gccaggatag gaccaaaacc ggaagcgata agaccaccaa
    42541 tttctttaga aatagccatc cgggtaaagc ggtttttaca gccgaacatt tctgccatgg
    42601 taatgttttc cagagcaaat aatcccagca ccgcacagtt atgaatcaca atcagtgcaa
    42661 ccataatggt gctcggggca tagcttttat ctacgatgat agaaagcatt ggccatgcca
    42721 gcacaatcgc ggaggtattc ataataatat acgggatccg gcgaccaatt ttatcggata
    42781 accaaccaag gaacggaatg gtcataaagc cgggaatcga actgatcatc aatgcatctg
    42841 ttggaattgc tttgttaaac aataacgtct gtactaaata gcctgcaagg aaagtctgaa
    42901 ttaacccgga gttacctgcc tgaccaaaac gcagccctgt tgccagccag aaggatttgc
    42961 tctggaatat gctaccagca ggtgcaggtt ttgctgtcgg ttggttgctg tcgttaacct
    43021 tctcaaagac cgggctttct ttcagattca tacgcaacca gatagcaaag accatcacga
    43081 caacgctcgc caggaacggt atacgccatc cccacgccag cagttcctct ttactgagaa
    43141 tgaagaacat aaaggcccag attgccgttg cgctcaaggt tccgcagtta gttcccatag
    43201 ccacaaatga ggagataatt ccgcgcttac cttttggcgc atattccgcc agcatcgtac
    43261 cggcaccgga aatttccgca cctgcaccca atccctgaat aatacgcagc gtcaccagca
    43321 agatgggggc aaaaacacca atctgtgcat aggtcggtaa cactccaatt aaggtggtac
    43381 agatccccat catggtgatg gtaataaaga gcactttttt acgccctatt ctgtcgccca
    43441 ttttgccgaa aataaatgct ccgacaatac gcgccacata acctgcaccg taggttccca
    43501 ttgccagaat taacgccatt gccgttgatg attcaggaaa aaatatttca tgaaacacta
    43561 acgctgcgcc gagcgaatat aactggaaat ccataattca tagatgtttt ttactattct
    43621 gtgggttttt gggtgttttc taagtttttt cagatggttg tattttttct aaaaatccct
    43681 aatctcgatt ttgctgttta tttgaggcct ttttatgtcc catatatgcc ccacagatac
    43741 cccgcagcca aaatcaacaa aatgccaaaa ggttctgttc ctgccctgca acaagaaatg
    43801 ctgcgacgtg tcagtaaacg ttatgacgat gtagaagtga tcatcaaatc caccagcaac
    43861 gatggccttt cagttactcg caccgccgat aaagattctg caaaaacttt tgttcaggag
    43921 acgctgaaag atacctggga gtctgctgac gagtggtttg ttcgctaatt aacgagtaaa
    43981 atcgaccctg tacacgattc tgtgtaaatg ccttttctca gaagtgaccg tccaggcggt
    44041 caccgaactc gataataaag cggctcattg ccatacgcca gtcccgcagt ggcatcgtcc
    44101 atttctgtga agccgcctgg attgccagcc acacgacctt tttcactgag tcatccgtcg
    44161 ggaacacctt gcgttttttg atggcatgcc ggattacgct gttcagcgac tcgatggcgt
    44221 tggttgtgta gatgactttg cggatatctg ccgggtaagc gaagaacatc gccaggttgg
    44281 tccagtttgc ctgccagctc cggcttatct gcgggtagcg actgtcccag gcgctggcga
    44341 acgcttccag cgcctgctgg cctgcttctt ctgtgggggc ctggtagatg gctttcaggt
    44401 cgcgggtgac ggctttgtag tccttccagg aaacgaaccg caggctgttg cgcaccatat
    44461 gcacgatgca cagctggatg cgggcttccg gatacaccgt gttgatagcg tccgggaagc
    44521 ctttcagccc gtcgacacag gctatgagaa tatcgttcag gccgcggttt tttagttcag
    44581 tcagcacatt gagccagaac ttcgctccct cgttttcggc cagccacata cccagtaact
    44641 ctttctggcc ttcgatattg atacccagtg cgaggaatac ggatttattg attacgcgac
    44701 tgtcctgccg aaccttcagg acaatacagt caagataaac aatgggatag accgcatcca
    44761 gtggccggtt ttgccattcg actacctgct ccataacggc atcggtgacc ttcgatacca
    44821 gcgccggtga gacatc
//
LOCUS       DABSYT020000026        43669 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.026, whole genome shotgun sequence.
ACCESSION   DABSYT020000026 DABSYT020000000
VERSION     DABSYT020000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 43669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.026"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            381..1598
                     /gene="alaA"
                     /locus_tag="ID725_RS09280"
                     /old_locus_tag="pgaptmp_001856"
     CDS             381..1598
                     /gene="alaA"
                     /locus_tag="ID725_RS09280"
                     /old_locus_tag="pgaptmp_001856"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGV7850468.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1682..2281
                     /gene="yfbR"
                     /locus_tag="ID725_RS09285"
                     /old_locus_tag="pgaptmp_001857"
     CDS             1682..2281
                     /gene="yfbR"
                     /locus_tag="ID725_RS09285"
                     /old_locus_tag="pgaptmp_001857"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGV7850469.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2340..4172)
                     /locus_tag="ID725_RS09290"
                     /old_locus_tag="pgaptmp_001858"
     CDS             complement(2340..4172)
                     /locus_tag="ID725_RS09290"
                     /old_locus_tag="pgaptmp_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGV7850470.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4259..4909)
                     /gene="hxpA"
                     /locus_tag="ID725_RS09295"
                     /old_locus_tag="pgaptmp_001859"
     CDS             complement(4259..4909)
                     /gene="hxpA"
                     /locus_tag="ID725_RS09295"
                     /old_locus_tag="pgaptmp_001859"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGV7850471.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4920..5414)
                     /locus_tag="ID725_RS09300"
                     /old_locus_tag="pgaptmp_001860"
     CDS             complement(4920..5414)
                     /locus_tag="ID725_RS09300"
                     /old_locus_tag="pgaptmp_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGV7850472.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5497..5952)
                     /gene="yfbV"
                     /locus_tag="ID725_RS09305"
                     /old_locus_tag="pgaptmp_001861"
     CDS             complement(5497..5952)
                     /gene="yfbV"
                     /locus_tag="ID725_RS09305"
                     /old_locus_tag="pgaptmp_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGV7850473.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6290..7492
                     /gene="ackA"
                     /locus_tag="ID725_RS09310"
                     /old_locus_tag="pgaptmp_001862"
     CDS             6290..7492
                     /gene="ackA"
                     /locus_tag="ID725_RS09310"
                     /old_locus_tag="pgaptmp_001862"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGV7850474.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7567..9711
                     /gene="pta"
                     /locus_tag="ID725_RS09315"
                     /old_locus_tag="pgaptmp_001863"
     CDS             7567..9711
                     /gene="pta"
                     /locus_tag="ID725_RS09315"
                     /old_locus_tag="pgaptmp_001863"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGV7850475.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9901..11421
                     /gene="yfcC"
                     /locus_tag="ID725_RS09320"
                     /old_locus_tag="pgaptmp_001864"
     CDS             9901..11421
                     /gene="yfcC"
                     /locus_tag="ID725_RS09320"
                     /old_locus_tag="pgaptmp_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGV7850476.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11454..11996)
                     /gene="yfcD"
                     /locus_tag="ID725_RS09325"
                     /old_locus_tag="pgaptmp_001865"
     CDS             complement(11454..11996)
                     /gene="yfcD"
                     /locus_tag="ID725_RS09325"
                     /old_locus_tag="pgaptmp_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGV7850477.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12054..12605)
                     /gene="yfcE"
                     /locus_tag="ID725_RS09330"
                     /old_locus_tag="pgaptmp_001866"
     CDS             complement(12054..12605)
                     /gene="yfcE"
                     /locus_tag="ID725_RS09330"
                     /old_locus_tag="pgaptmp_001866"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGV7850478.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12661..13305)
                     /gene="yfcF"
                     /locus_tag="ID725_RS09335"
                     /old_locus_tag="pgaptmp_001867"
     CDS             complement(12661..13305)
                     /gene="yfcF"
                     /locus_tag="ID725_RS09335"
                     /old_locus_tag="pgaptmp_001867"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGV7850479.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13441..14088
                     /gene="yfcG"
                     /locus_tag="ID725_RS09340"
                     /old_locus_tag="pgaptmp_001868"
     CDS             13441..14088
                     /gene="yfcG"
                     /locus_tag="ID725_RS09340"
                     /old_locus_tag="pgaptmp_001868"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGV7850480.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14145..14507
                     /gene="folX"
                     /locus_tag="ID725_RS09345"
                     /old_locus_tag="pgaptmp_001869"
     CDS             14145..14507
                     /gene="folX"
                     /locus_tag="ID725_RS09345"
                     /old_locus_tag="pgaptmp_001869"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGV7850481.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14528..15421
                     /locus_tag="ID725_RS09350"
                     /old_locus_tag="pgaptmp_001870"
     CDS             14528..15421
                     /locus_tag="ID725_RS09350"
                     /old_locus_tag="pgaptmp_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGV7850482.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15469..16359)
                     /gene="rpnB"
                     /locus_tag="ID725_RS09355"
                     /old_locus_tag="pgaptmp_001871"
     CDS             complement(15469..16359)
                     /gene="rpnB"
                     /locus_tag="ID725_RS09355"
                     /old_locus_tag="pgaptmp_001871"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGV7850483.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16556..17329)
                     /gene="hisP"
                     /locus_tag="ID725_RS09360"
                     /old_locus_tag="pgaptmp_001872"
     CDS             complement(16556..17329)
                     /gene="hisP"
                     /locus_tag="ID725_RS09360"
                     /old_locus_tag="pgaptmp_001872"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGV7850484.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17337..18053)
                     /locus_tag="ID725_RS09365"
                     /old_locus_tag="pgaptmp_001873"
     CDS             complement(17337..18053)
                     /locus_tag="ID725_RS09365"
                     /old_locus_tag="pgaptmp_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7850485.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18050..18736)
                     /gene="hisQ"
                     /locus_tag="ID725_RS09370"
                     /old_locus_tag="pgaptmp_001874"
     CDS             complement(18050..18736)
                     /gene="hisQ"
                     /locus_tag="ID725_RS09370"
                     /old_locus_tag="pgaptmp_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGV7850486.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18826..19608)
                     /gene="hisJ"
                     /locus_tag="ID725_RS09375"
                     /old_locus_tag="pgaptmp_001875"
     CDS             complement(18826..19608)
                     /gene="hisJ"
                     /locus_tag="ID725_RS09375"
                     /old_locus_tag="pgaptmp_001875"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGV7850487.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19829..20611)
                     /gene="argT"
                     /locus_tag="ID725_RS09380"
                     /old_locus_tag="pgaptmp_001876"
     CDS             complement(19829..20611)
                     /gene="argT"
                     /locus_tag="ID725_RS09380"
                     /old_locus_tag="pgaptmp_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGV7850488.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20877..21446)
                     /gene="ubiX"
                     /locus_tag="ID725_RS09385"
                     /old_locus_tag="pgaptmp_001877"
     CDS             complement(20877..21446)
                     /gene="ubiX"
                     /locus_tag="ID725_RS09385"
                     /old_locus_tag="pgaptmp_001877"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGV7850489.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21541..23058)
                     /gene="purF"
                     /locus_tag="ID725_RS09390"
                     /old_locus_tag="pgaptmp_001878"
     CDS             complement(21541..23058)
                     /gene="purF"
                     /locus_tag="ID725_RS09390"
                     /old_locus_tag="pgaptmp_001878"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGV7850490.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23095..23583)
                     /gene="cvpA"
                     /locus_tag="ID725_RS09395"
                     /old_locus_tag="pgaptmp_001879"
     CDS             complement(23095..23583)
                     /gene="cvpA"
                     /locus_tag="ID725_RS09395"
                     /old_locus_tag="pgaptmp_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGV7850491.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(24115..24777)
                     /gene="dedD"
                     /locus_tag="ID725_RS09400"
                     /old_locus_tag="pgaptmp_001880"
     CDS             complement(24115..24777)
                     /gene="dedD"
                     /locus_tag="ID725_RS09400"
                     /old_locus_tag="pgaptmp_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGV7850492.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24767..26035)
                     /gene="folC"
                     /locus_tag="ID725_RS09405"
                     /old_locus_tag="pgaptmp_001881"
     CDS             complement(24767..26035)
                     /gene="folC"
                     /locus_tag="ID725_RS09405"
                     /old_locus_tag="pgaptmp_001881"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGV7850493.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(26105..27019)
                     /gene="accD"
                     /locus_tag="ID725_RS09410"
                     /old_locus_tag="pgaptmp_001882"
     CDS             complement(26105..27019)
                     /gene="accD"
                     /locus_tag="ID725_RS09410"
                     /old_locus_tag="pgaptmp_001882"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGV7850494.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(27175..27834)
                     /locus_tag="ID725_RS09415"
                     /old_locus_tag="pgaptmp_001883"
     CDS             complement(27175..27834)
                     /locus_tag="ID725_RS09415"
                     /old_locus_tag="pgaptmp_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGV7850495.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27917..28729)
                     /gene="truA"
                     /locus_tag="ID725_RS09420"
                     /old_locus_tag="pgaptmp_001884"
     CDS             complement(27917..28729)
                     /gene="truA"
                     /locus_tag="ID725_RS09420"
                     /old_locus_tag="pgaptmp_001884"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGV7850496.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28729..29742)
                     /locus_tag="ID725_RS09425"
                     /old_locus_tag="pgaptmp_001885"
     CDS             complement(28729..29742)
                     /locus_tag="ID725_RS09425"
                     /old_locus_tag="pgaptmp_001885"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGV7850497.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29808..30944)
                     /gene="pdxB"
                     /locus_tag="ID725_RS09430"
                     /old_locus_tag="pgaptmp_001886"
     CDS             complement(29808..30944)
                     /gene="pdxB"
                     /locus_tag="ID725_RS09430"
                     /old_locus_tag="pgaptmp_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGV7850498.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            31043..32038
                     /gene="flk"
                     /locus_tag="ID725_RS09435"
                     /old_locus_tag="pgaptmp_001887"
     CDS             31043..32038
                     /gene="flk"
                     /locus_tag="ID725_RS09435"
                     /old_locus_tag="pgaptmp_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGV7850499.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQSEAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(32035..33213)
                     /locus_tag="ID725_RS09440"
                     /old_locus_tag="pgaptmp_001888"
     CDS             complement(32035..33213)
                     /locus_tag="ID725_RS09440"
                     /old_locus_tag="pgaptmp_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850500.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33488..34708)
                     /gene="fabB"
                     /locus_tag="ID725_RS09445"
                     /old_locus_tag="pgaptmp_001889"
     CDS             complement(33488..34708)
                     /gene="fabB"
                     /locus_tag="ID725_RS09445"
                     /old_locus_tag="pgaptmp_001889"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGV7850501.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34867..36873
                     /gene="mnmC"
                     /locus_tag="ID725_RS09450"
                     /old_locus_tag="pgaptmp_001890"
     CDS             34867..36873
                     /gene="mnmC"
                     /locus_tag="ID725_RS09450"
                     /old_locus_tag="pgaptmp_001890"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGV7850502.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36994..37272)
                     /locus_tag="ID725_RS09455"
                     /old_locus_tag="pgaptmp_001891"
     CDS             complement(36994..37272)
                     /locus_tag="ID725_RS09455"
                     /old_locus_tag="pgaptmp_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGV7850503.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37306..37854)
                     /gene="epmC"
                     /locus_tag="ID725_RS09460"
                     /old_locus_tag="pgaptmp_001892"
     CDS             complement(37306..37854)
                     /gene="epmC"
                     /locus_tag="ID725_RS09460"
                     /old_locus_tag="pgaptmp_001892"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGV7850504.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37854..38663)
                     /locus_tag="ID725_RS09465"
                     /old_locus_tag="pgaptmp_001893"
     CDS             complement(37854..38663)
                     /locus_tag="ID725_RS09465"
                     /old_locus_tag="pgaptmp_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGV7850505.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38663..39487)
                     /gene="mepA"
                     /locus_tag="ID725_RS09470"
                     /old_locus_tag="pgaptmp_001894"
     CDS             complement(38663..39487)
                     /gene="mepA"
                     /locus_tag="ID725_RS09470"
                     /old_locus_tag="pgaptmp_001894"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGV7850506.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39491..40576)
                     /gene="aroC"
                     /locus_tag="ID725_RS09475"
                     /old_locus_tag="pgaptmp_001895"
     CDS             complement(39491..40576)
                     /gene="aroC"
                     /locus_tag="ID725_RS09475"
                     /old_locus_tag="pgaptmp_001895"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGV7850507.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40611..41543)
                     /gene="prmB"
                     /locus_tag="ID725_RS09480"
                     /old_locus_tag="pgaptmp_001896"
     CDS             complement(40611..41543)
                     /gene="prmB"
                     /locus_tag="ID725_RS09480"
                     /old_locus_tag="pgaptmp_001896"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGV7850508.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            41709..42260
                     /gene="smrB"
                     /locus_tag="ID725_RS09485"
                     /old_locus_tag="pgaptmp_001897"
     CDS             41709..42260
                     /gene="smrB"
                     /locus_tag="ID725_RS09485"
                     /old_locus_tag="pgaptmp_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGV7850509.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42431..43273)
                     /locus_tag="ID725_RS09490"
                     /old_locus_tag="pgaptmp_001898"
     CDS             complement(42431..43273)
                     /locus_tag="ID725_RS09490"
                     /old_locus_tag="pgaptmp_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGV7850510.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(43275..>43669)
                     /locus_tag="ID725_RS09495"
                     /old_locus_tag="pgaptmp_001899"
     CDS             complement(43275..>43669)
                     /locus_tag="ID725_RS09495"
                     /old_locus_tag="pgaptmp_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7850511.1"
                     /translation="NLLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKV
                     TLMGTEDTDLPGFLSIDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNF
                     NAWLQTVNGRNVTSGDFTATMTVTFEYF"
BASE COUNT        10818 a        11786 c        10878 g        10187 t
ORIGIN      
        1 gcttaacaat agtttcacac cttgccccca ttgcgacacc tcggaaatca tcacgctgtt
       61 aaactctccg taatgcggaa atatcatcat cgcgtttcat tagtgaattc ttaaatgagg
      121 cattttcaca caattatctt acagataaaa aaaccagact tacaattaag aatcagaaca
      181 attcactata taacattgca tgtaaagcat atgcacctca ttattttgtc attattaagt
      241 tattaacagc acaatcgagc cttcccctct ggcaaaatct tattctgcag accttcaaaa
      301 caccgtcctg ggggagtaca ttgttctaag ctgacttcca cggcagggag tggcgataac
      361 agcaaaaaag gtcaagattc atgtccccca ttgaaaaatc cagcaaatta gagaatgtct
      421 gttatgacat ccgtggtccg gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca
      481 aggtactgaa actgaacatc ggcaacccag ccccgttcgg ttttgacgcg ccagatgaaa
      541 tcctcgttga cgtgatacgc aacctgccta ccgctcaagg gtattgcgat tccaaaggtc
      601 tttactccgc gcgtaaagcc atcatgcagc actatcaggc tcgtggcatg cgtgatgtta
      661 ccgtggaaga tatttacatc ggcaatggtg tatcggagct tatcgttcag gcaatgcagg
      721 cattgctgaa cagcggggac gaaatgttgg ttcctgcacc agattaccca ctatggaccg
      781 cggcggtttc gctttccagc ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact
      841 ggttcccgga cctcgatgat attcgcgcta aaattacgcc tcgtacgcgt gggatcgtta
      901 ttatcaaccc aaataaccca accggcgcgg tatattccaa agagctttta atggagattg
      961 tggagattgc acgtcagcat aatctcatta tcttcgccga tgaaatttat gacaaaattc
     1021 tctacgacga cgctgagcat cactcaattg cgccgctggc acctgacctg ctgaccatta
     1081 cctttaatgg actgtcgaaa acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt
     1141 tgaacgggcc gaaaaaacac gccaaaggct acatcgaagg tctggaaatg ctggcttcaa
     1201 tgcgcctgtg tgctaacgtt cctgcgcaac acgccattca gaccgcgctg ggtggttatc
     1261 agagcatcag tgaatttatt acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt
     1321 gggaattgat caacgatatt ccgggcgttt cctgcgtgaa accttgtggt gcgctgtaca
     1381 tgttcccgaa aatcgacgcc aaacgcttta acattcacga cgatcagaaa atggtgctgg
     1441 atttcctgtt gcaggaaaaa gttctgttgg tgcaagggac ggcattcaac tggccgtggc
     1501 cggatcactt ccgcattgtc acgctaccgc gtgtcgatga tatcgagctg tcgttgagca
     1561 agttcgcgcg tttcctttct ggttatcatc agctgtaatc ttaatttcac tgccggagat
     1621 tgcatccggc agtgttatcc cgccacaatg acctgatgat gtcatcatac gtaaggtcac
     1681 tatgaaacag agccattttt ttgcccatct ctcccgcctg aaactcatta accgctggcc
     1741 gctcatgcgc aacgtgcgga cggaaaatgt gtccgaacac agtttgcagg tagcgatggt
     1801 cgcccatgcg ctggcagcta tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg
     1861 tatcgcttta ctggcgatgt accacgatgc ctcagaagtg ctcaccggcg atctccctac
     1921 tccggtgaaa tacttcaatt cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc
     1981 tcagcaaaaa ctggtcgata tggttccgga agagctgcgg gatatctttg cgccgttaat
     2041 tgacgagcat gcatatagcg atgaagaaaa atcgctggtg aaacaggcag atgcactgtg
     2101 tgcatatctg aaatgtctgg aagaactcgc ggcgggaaat aatgaattct tgctggcaaa
     2161 aacgcgactg gaagcgacgc ttgaagcgcg ccgcagccag gagatggact acttcatgga
     2221 agtatttgtt cccagcttcc atctttcgct cgatgagatt agccaggatt caccgctgta
     2281 agcagctgga gtctgcgtcg catcaggcaa taagcgccgg atgcgacatc aggctcttgt
     2341 caaaacggaa acagcatcgg gatcatcacc acacaaaccg ccatcacgat aatggtgaac
     2401 ggtaccccca gcttcacaaa gtcactgaag ctgtaatttc ccggacctaa aaccaatgtg
     2461 ttaacaggtg aagaaaccgg tgtcataaag gcggcggatg ctgccatcgc cacgaccatc
     2521 gcgaatggat aaggcgacac ccccatcgtt ttggcagcag ccagcgcaat cggagccatc
     2581 aacaccgccg tcgcggtatt agagataaat agcccaataa ccgccgacaa gacaaacaaa
     2641 caacccagca tcatatgtgg cccgtaacca ccgccaatgt ccatcagccc tttcaccgcc
     2701 agcgcgacac ctcccgtttt ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa
     2761 ataatgctcg gccagtgaat ggatttatag gcgctttcag catctataca gcggaatttc
     2821 cccatcagca ggcaggcgat gatagcggca acaggattag gaatttcatc tgtcagcatt
     2881 aacgccacca ttagcaccag acagaaaatg gcatggggtg cctggctgtg cgcgggcgat
     2941 gcttcactca cctcttccgg taagttcagc gctacgaagt cgcggccctg tttggccagc
     3001 ataccgatca gtttccagtt acccacaacc aggatgatat cgcccagcag cagaggctca
     3061 tccgccagcg aaccttccag cgccacgcca ttgcgcttta gccccaccac attcagtccg
     3121 tagcgggtac gaaaaccaat ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt
     3181 aatgaaatct ctgccatgcc cacatcaagg gcctggtcag aaaaatactc gccgcgcagt
     3241 accatcggct ccagcaattg ctcactacaa aattgccgga gatcgacatc agccgcagac
     3301 atatcaataa gcaaaacgtc acgcgcgcga aattcagaaa ccccattaac gttcacgata
     3361 acgcgacgaa aacgccgcca gcgttcaaca ccgatgacgt tagcgccata acgctcacgt
     3421 aatttgagat catccagccg ttgaccaatc attggcgatc cggggcgaat agccagacgt
     3481 cgcgcacgcc cggtcagtcg atattcacgg ataagatcgc gaaaggttcg acgcgtccag
     3541 ccttcgcgct gcggggtctg ggtatcccct ttcagcatga aacgcatcac taacatatac
     3601 aaaataccca gcaccagcac gaccaggcca ataggtgtta cgctaaagaa actgaagccg
     3661 tgatagcctt cacgcagcaa ttcactgttg actaccaggt tcggcggcgt cgccaccagc
     3721 gtcatcatgc cgctaatcag cccggcaaaa cttaacggca tcatcagacg tgacggcgaa
     3781 gtttgcatac gcatggcaac gcttaacacc acgggaataa agatagcgac aacgccggtt
     3841 gaactcataa acgcgccaag ccccgcgacg gtcagcatca acaaaaccaa cattttgatt
     3901 tcactgttgc ccgcaacttt gaccagccat gttcccatta cggtggcaac accggtacgg
     3961 accaaaccat cgccaataat aaacaaggcg gcaatcagga caacgttagg atcagaaaag
     4021 ccagaaaata cttctgggac tgtcagcgtt ccgcttaatg caaacgcgac aataacaaac
     4081 aaagcgaccg catccatacg cactctgccc gtcgcaaaca agacgatggc aacggccagt
     4141 aatgaaagaa cccaaatcaa ttcaccgttc acaacttatc cttgttaatt gagggggatg
     4201 acttgattct gccataaaaa agccccgacg agacggggct aaatcatgat caggtgtttc
     4261 actgaataat aacatcgcca tttggctgtt tggtcacagt aatttgctcc agactgtgga
     4321 ggaccaaatc gacctcattc aggcgcgggg tatctgccgg agcgttaacc gcaatgacat
     4381 ggcaacccgc cgccaggcca gaaagcacgc cagcgggagc atcttccacc accacacact
     4441 cctgcggcgc aagccccagc agctgcgcgc ctaacagata cgcatcaggt tctggttttc
     4501 cgcgcttcac tcgctcagcg gttacaaaca cctctggtgc gggaagcccc gctattttat
     4561 ggcgcgctcg cgctaccggc atggagccag aagtcacaat ggcccacgga atacctgctt
     4621 tattcaaatg attgagtaag gcgattgccc ccggaagcgc ggtaatacct tcggtttccg
     4681 tggcctcgat ttgctccaaa cgcgtaaact cggcggcaat atcagcctcg gatttgcccg
     4741 ccataaaatg gcgcagagag gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt
     4801 ccggcgctaa cccatgacgt ctggcccagt tgctccacgc ccgttctacc gcaggcaggg
     4861 aatccaccag cgttccatca agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct
     4921 caggcgttga taatttgatt aatttcgttg gcgctcaaat ggtactgacg cgggcaggca
     4981 tgccacacat taagcatgcg ctgatatttt tcccacattg gcgtctgggc gttaaaaccg
     5041 tgagttccgg cgtcaaaatg ggtatagcgc ccttccacat taaccataaa gcggacataa
     5101 ccgaggtaac gtgcttcagt ggcggcgtca aagccgagga aggtgacacg acgttcatcg
     5161 atggattgct ggtcctgcaa attagaccag gaaacatgca acgcatgata catctccata
     5221 atgtcgatga tagtgcggca ggtttcttct ttcagctcgc caaactcgcg atccagttca
     5281 cgcatctgta atccataacc ccgctcaatg attgtttgca ggcgacggta acgttcagca
     5341 tttgccggat cgagcatagt catcatcttg tactggttag acaaaataag acgttgcgcg
     5401 ttggtcattt ccattgttga ctcctgtatc actctactac ggtgaaaaaa aagaaggctg
     5461 agtatgcctt cttttatatg cgtaatcagg ggtcaattac aaatcatcaa ggaaagtttt
     5521 atccagttgt ttgaaggcac gcttaagcgt gtcagctaat gcctggtaat caggcttgcc
     5581 ttcaacgggt gccaacacct gtccagactc ctgcaattta ccgcgaactt cataaaacca
     5641 gttgaggatt gcagggggta atggcgtgac agaacgcttg cccagccacc acaatccctg
     5701 catgggtaaa cttaaggcga acagggcagt ggcaactgcc ggcccaagct gaccgcccag
     5761 ggcaatctgc cagcagagag taaatacggc gatcggcggc ataaaacgga tcgcataacg
     5821 cgtcatcttg ataacgcgat tttcgacaaa gaccggggca aggcgttttt ccagcggcca
     5881 cgtctttgag taatgctgtc cccggcgaaa caagctaaaa aaattaacag aacgattatc
     5941 cggcgttgac atgcttcacc tcaacttcac atataaagat tcaaaaattt gtgcaaattc
     6001 acaactcagc gggacaacgt tcaaaacatt ttgtcttcca tacccactat caggtatcct
     6061 ttagcagcct gaaggcctaa gtagtacata ttcattgagt cgtcaaattc atatacatta
     6121 tgccattggc tgaaaattac gcaaaatggc atagactcaa gatatttctt ccatcatgca
     6181 aaaaaaattt gcagtgcatg atgttaatca taaatgtcgg tgtcatcatg cgctacgctc
     6241 tatggctccc tgacgttttt ttagccacgt atcaattata ggtacttcca tgtcgagtaa
     6301 gttagtactg gttctgaact gcggtagttc ttcactgaaa tttgccatca tcgatgcagt
     6361 aaatggtgaa gagtaccttt ctggtttagc cgaatgtttc cacctgcccg aagcacgtat
     6421 caaatggaaa atggacggca ataaacagga agcggcttta ggtgcaggcg ccgctcacag
     6481 cgaagcgctc aactttatcg ttaatactat tctggcacaa aaaccagaac tgtctgcgca
     6541 gctgactgct atcggtcacc gtatcgtaca cggcggcgaa aagtatacca gctccgtagt
     6601 gatcgatgag tctgttattc agggtatcaa agatgcagct tcttttgcac cgctgcacaa
     6661 cccggctcac ctgatcggta tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa
     6721 aaacgttgct gtatttgaca ccgcgttcca ccagactatg ccggaagagt cttacctcta
     6781 cgccctgccg tacaacctgt acaaagagca cggcatccgt cgttacggcg cgcacggcac
     6841 cagccacttc tatgtaaccc aggaagcggc aaaaatgctg aacaaaccgg tagaagaact
     6901 gaacatcatc acctgccacc tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa
     6961 atgcgttgac acctctatgg gcctgacccc gctggaaggt ctggtcatgg gtacccgttc
     7021 tggtgatatc gatccggcga tcatcttcca cctgcacgac accctgggca tgagcgttga
     7081 cgcaatcaac aaactgctga ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag
     7141 cgactgccgc tatgttgaag acaactacgc gacgaaagaa gacgcgaagc gcgcaatgga
     7201 cgtttactgc caccgcctgg cgaaatacat cggtgcctac actgcgctga tggatggtcg
     7261 tctggacgct gttgtattca ccggtggtat cggtgaaaat gccgcgatgg tccgtgaact
     7321 gtctctgggc aaactgggcg tgctgggctt tgaagttgat catgaacgca atctggctgc
     7381 acgtttcggc aaatctggtt tcatcaacaa agaaggtacc cgtcctgcgg tggttatccc
     7441 aaccaacgaa gaactggtta tcgcgcaaga cgcgagccgc ctgactgcct gatttcacac
     7501 cgccagctca gctggcggtg ctgttttgta acccgccaaa tcggcggtaa cgaaagagga
     7561 taaaccgtgt cccgtattat tatgctgatc cctaccggaa ccagcgtcgg tctgaccagc
     7621 gtcagccttg gcgtgatccg tgcaatggaa cgcaaaggcg ttcgtctgag cgttttcaaa
     7681 cctatcgctc agccgcgtac cggtggcgat gcgcccgatc agactacgac tatcgtgcgt
     7741 gcgaactctt ccaccacgac ggccgctgaa ccgctgaaaa tgagctacgt tgaaggtctg
     7801 ctttccagca atcagaaaga tgtgctgatg gaagagatcg tcgcaaacta ccacgctaac
     7861 accaaagacg ctgaagtcgt tctggttgaa ggtctggtcc cgacacgtaa gcaccaattt
     7921 gcccagtctc tgaactacga aatcgctaaa acgctgaatg cggaaatcgt cttcgttatg
     7981 tctcagggca ctgacacccc ggaacagctg aaagagcgta tcgaactgac ccgcaacagc
     8041 ttcggcggtg ccaaaaacac caacatcacc ggcgttatcg ttaacaaact gaacgcaccg
     8101 gttgatgaac agggtcgtac tcgcccggat ctgtccgaga ttttcgacga ctcttccaaa
     8161 gctaaagtaa acaatgttga tccggcgaag ctgcaagaat ccagcccgct gccggttctc
     8221 ggcgctgtgc cgtggagctt tgacctgatc gcgactcgtg cgatcgatat ggctcgccac
     8281 ctgaatgcga ccatcatcaa cgaaggcgac atcaatactc gccgcgttaa atccgtcact
     8341 ttctgcgcac gcagcattcc gcacatgctg gagcacttcc gtgccggttc tctgctggtg
     8401 acttccgcag accgtcctga cgtgctggtt gccgcttgcc tggctgccat gaacggcgta
     8461 gaaatcggtg ccctgctgct gactggcggc tacgaaatgg acgcgcgcat ttctaaactg
     8521 tgcgaacgtg ctttcgctac cggcctgccg gtatttatgg tgaacaccaa cacctggcag
     8581 acttctctga gcctgcagag cttcaacctg gaagttccag ttgacgatca cgagcgtatc
     8641 gagaaagttc aggaatacgt tgctaactac atcaacgctg actggatcga ttctctgact
     8701 gccacttctg agcgcagccg tcgtctgtct ccgccagcgt tccgttatca gctgactgaa
     8761 cttgcgcgca aagcgggcaa acgtatcgta ctgccggaag gtgacgaacc gcgtaccgtt
     8821 aaagcagccg ctatctgtgc tgaacgtggt atcgcaactt gcgtactgct gggtaatccg
     8881 gcagagatca accgtgttgc agcgtctcag ggtgtagaac tgggtgcagg cattgaaatc
     8941 gttgatccag aagtggttcg cgaaagctat gttggtcgtc tggtcgagct gcgtaagaac
     9001 aaaggcatga ccgaaaccgt tgcccgcgaa cagctggaag acaacgtggt gctcggtacg
     9061 ctgatgctgg aacaggatga agttgatggt ctggtttccg gtgctgttca cactaccgca
     9121 aacaccatcc gtccgccgct gcagctgatc aaaactgcac cgggcagctc cctggtatct
     9181 tccgtgttct tcatgctgct gccggaacag gtttacgttt acggtgactg tgcgatcaac
     9241 ccggatccga ccgcagaaca gctggcagaa atcgcgattc agtccgctga ttccgctgca
     9301 gccttcggta tcgaaccgcg cgttgctatg ctctcctact ctaccggtac ttctggtgca
     9361 ggtagcgacg tagaaaaagt tcgcgaagca actcgtctgg cgcaggaaaa acgtcctgac
     9421 ctgatgatcg acggtccgct gcagtacgac gctgcggtaa tggctgacgt tgcgaaatcc
     9481 aaagcgccga actctccggt tgcaggtcgc gctaccgtgt tcatcttccc ggatctgaac
     9541 accggtaaca ccacctacaa agcggtacag cgttctgccg acctgatctc catcgggccg
     9601 atgctgcagg gtatgcgcaa gccggttaac gacctgtccc gtggcgcact ggttgacgat
     9661 atcgtctaca ccatcgcgct gactgcgatt cagtctgcac agcagcagta acctcgtcat
     9721 catccgcagc tttgcgctgc ggatatctga accggaaata gtcactattt ccggtttttt
     9781 attctcttaa tttgcattaa tcctttctga ttatcttgct taactgcgct gcatcaatga
     9841 attgcgccat ctcactttgc atacttacca ctttgttttg tgcaagggaa tatttgcgct
     9901 atgtccgcaa tcactgaatc caaaccaaca agaagatggg caatgcccga tacgttggtg
     9961 attatctttt ttgttgctat tttaaccagc cttgccacct gggtagttcc ggtggggatg
    10021 tttgacagtc aggaagtgca gtatcaggtt gatggtcaaa caaaaacacg caaagtcgta
    10081 gatccacact catttcgcct tctgactaac gaagcaggcg aacctgagta tcaccgcgta
    10141 cagctgttca cgacgggcga tgaacgcccg ggcctgatga acttcccgtt tgaagggtta
    10201 acctcaggat cgaaatacgg gacagccgtt ggcatcatca tgtttatgct ggtgattggc
    10261 ggcgcgtttg gcattgtgat gcgtacagga accattgata acggtatcct ggcgcttatt
    10321 cgccataccc gtgggaatga aattctcttt attcctgcgc tgtttattct gttttcactt
    10381 ggcggcgcgg tatttggtat gggggaagag gccgtcgcct ttgccattat catcgcaccg
    10441 ctaatggtcc ggctgggcta tgacagtatt accaccgtcc tggtgaccta tattgccacg
    10501 caaatcggtt ttgccagttc gtggatgaac ccgttttgtg tggtcgttgc tcaggggatt
    10561 gccggcgttc cggtgctttc tggctccggg ttgcgcatcg tggtgtgggt tatcgccact
    10621 ctgattggcc tgatctttac catggtgtac gcctcacgag tgaaaaagaa tcctcttctg
    10681 tcacgcgtgc atgagtccga ccgcttcttt cgtgaaaagc aggcggatgt tgaacaacgt
    10741 ccgtttacct ttggtgactg gctggtattg attgtcctga ccgccgtaat ggtctgggtg
    10801 atttggggcg tgatcgttaa tgcctggttt attccagaaa ttgccagcca gttcttcacc
    10861 atgggtctgg tgattggcat catcggcgtt gttttccgcc ttaacggtat gacggttaat
    10921 accatggctt catccttcac cgaaggggcg cgaatgatga tcgcccctgc cctgctggtg
    10981 ggtttcgcca aagggatttt gctgctggtc ggtaatggtg aagcgggtga tgccagcgtg
    11041 ttaaatacta tcctcaacag cattgccaat gccattagcg gtctggacaa cgcggtcgcg
    11101 gcctggttta tgttgctctt ccaggcggta tttaatttct tcgtgacgtc cggttctggt
    11161 caggcggcgt taaccatgcc gttactggca ccgcttggcg atctggtcgg tgttaaccgt
    11221 caggttaccg tgctggcttt ccagtttggt gatggcttca gtcacatcat ttacccgacc
    11281 tcagcttcgt taatggcgac gctcggtgtt tgccgggtgg acttccgtaa ctggctgaag
    11341 gtgggcgcga ccctgcttgg actgctgttt attatgtcca gcgtcgtggt gatcggcgct
    11401 cagttgatgg gctaccacta aaaatgttaa gagccgcaat tgcggctctt ttttcattct
    11461 gccgtttcag tctctacggc ttcatttttg gcattgcgct tcatccacaa cgctagcgct
    11521 ttcagcgagt ctggagtgaa ctcatcgcag cgtgcggtga tttcttccgg cgtcagccag
    11581 caaacttcac tgacttcatc ttcctgtagg gcgaagggac cgtgagagac gcagctgaac
    11641 aatgcgcccc agacacggca atttttatct tcgaaataga actgcccgtg ctcggcaaag
    11701 gggacaccgg caatgcccaa ctcttcttcc gcttcgcgac gcgcggattc cagcagttgc
    11761 tcatcggcct ggactacacc gccagcggtc gcatctaaca tgccgggtaa aaagtctttt
    11821 gtctcggtac gacgctggac cagaattttg cccatgccat catgcacgac gatgtaagtt
    11881 gcacgatgac gcagacactg tgcccgcatt tgctcccggc tggcttgtgc aatgacttcg
    11941 ttctcttcat tgacaatatc cacccattca gtacttgcca aacgacgctg ttccattatc
    12001 aggaaacctt ctttttctgg cgctcttacg gcgcattttg agttgtgggt aaattacgga
    12061 ttaatcgcga cctgcgcaat gatactttga tcattgagtg cgattacact aagtacgtca
    12121 ttatccagca tgccataact cgccggatta ccgcctttcg gaatactcac cgagccgggg
    12181 ttgaagtgga aaatctcccc ccgctgttct gctactggca gatgggtatg accgtaaacc
    12241 agcacatcgt tctggtttaa agcaggcaga ttttccgggc caaaaagatg accatgcgtc
    12301 aaaaacagac gttgtttttc cagtaatacc tgttgccacg gcgcggttat cgggaaatgc
    12361 agcagcattt gatccacttc gctgtcgcag ttgccgcgca cagcgataac cttatgtgcc
    12421 acttcattaa gccgttcagc gactttggct ggcgcgtaac cctccggtaa agcattacgc
    12481 gggccgtgat tcaacacgtc gccaaggatc accagccact gggcaccgct ttgggcaaac
    12541 aactccagaa cacgttccgt cgccggtaac gacccatgaa tgtccgatgc aaacatcagt
    12601 ttcatcactc actcctcgtc gaaaaaacgt aagctatgat actggattct gtcgttgcta
    12661 tcagccagat tgcttcgctg aaagtgcaat aaaacgctgg acagacgctc gctgccactg
    12721 gaatgttgca taatccacca ggcattctgg cacctcatcg ccatgtagta ccaggcggtt
    12781 aatcattagc gccagatcag tatcagcaat gcaccattca ccaaataaat tcggctgacc
    12841 cagtactaac aaatgttctg ccatcgcgaa cagtttctct gcactggctt ttccctcggc
    12901 cgttagtggc gcttttttcg cccccgcaaa gacaacatct gtcggacgct cttcgcggat
    12961 gggcatcaga tcgctgcgca accaggcctg aatctgtcgt gcacgcgcac gattttctaa
    13021 atcaagcgga taaatacgtt cccaggtcgg tggcgcaaat cgatcttcca gatactccgc
    13081 aatggcagaa gattcactca actcaaaatc atcgatttgt aataacggca cacggcgtgt
    13141 ctgaccgtaa ccttgccacg tcggttgcaa atgttcaccg ctgtcgaggt cgatggtctt
    13201 gatatgaaac aacaggcctt tttcctgcaa cgccacccaa gcggataaca cataagggga
    13261 gaaaaaatgg gcatctgacc aaagcgtgat agcgggttta ctcattatgt cctcggcaat
    13321 aaagggggct ggctatcaaa ctatagagcc tttttgtcac tgtcacgtga cgaatactca
    13381 cgtaagtcgc cgcgctttct ataattgtgg tgttcagatg attagacaac cggagctcca
    13441 atgatcgatc tctatttcgc cccgacaccc aatggccaca aaattacgct gtttctcgaa
    13501 gaagcagggc tggattatcg cttgataaag gtagacctgg ggaaaggcgg tcagtttcgc
    13561 ccggaatttt tgctcatttc gcctaacaac aaaattccgg caattgttga tcattctcca
    13621 gccgatggcg gcgaaccgct aagcctcttt gaatctggtg ccattttgtt gtatctggct
    13681 gagaaaacgg ggctcttttt gagtcatgaa acgcgtgaac gcgccgccac attacagtgg
    13741 ttattctggc aggtaggcgg actggggccg atgcttgggc aaaatcatca ttttaatcac
    13801 gcagcccccc aaaccattcc ttacgctatt gaacgttatc aggttgaaac tcagcgtctg
    13861 taccatgtac tgaacaagcg gctggaaaac tcgccctggc tgggaggcga gaactacagc
    13921 attgcggata ttgcctgctg gccgtgggtt aatgcctgga ctcgccagcg aatagacctc
    13981 gctatgtatc cggcagtcaa gaactggcat gagcggatcc gttcgcgccc tgccaccggg
    14041 caggcactgc taaaagcaca actcggtgat gagcgttcgg atagttaaca gaaacaggtt
    14101 ctcgtgtatt atttcatcct aagtaaaaca acggagaacc tgcaatggca caacctgccg
    14161 ctattattcg tataaagaac cttcgtttac gtacatttat cggaattaag gaagaagaaa
    14221 ttaacaaccg tcaggatatt gttatcaatg tgacgatcca ctaccccgcc gataaagcgc
    14281 gcactagcga agatatcaac gatgcgctga attatcgcac cgtaacgaaa aacattattc
    14341 agcatgtaga gaataaccgt ttctctttgc tggaaaaatt aactcaggat gtgctcgata
    14401 tcgcacgtga acatcactgg gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc
    14461 tgcgctacgc cgattcggta tccatgacct taagctggca gcgttaaccg ccatatcggg
    14521 aggctgcatg aacatagtga tcaccggagg gacgggatta attggtcgcc atttgattcc
    14581 acgtttgctg gagctgggcc atcaaatcac ggtaatgacg cgtaacccgc agaaagccag
    14641 ttccgttctc ggccctcggg tgacactatg gcaagggctt gccgatcaaa gcaacctcaa
    14701 cggcattgat gcggtaatca acctggccgg agaaccgatt gctgataaac gttggactca
    14761 cgagcaaaaa gagcgtctct gccaaagccg ctggaatatc acgcaaaaac tggtcgattt
    14821 gattaatgcc agcgacacgc caccgtcggt actcatttcc ggctcggcaa cgggctatta
    14881 tggcgactta ggtgaagtgg tggttaccga agaggaaccg ccgcataaca aatttaccca
    14941 taaactctgc gcccgctggg aagaaattgc ctgtcgggcg caaagtgaca aaacgcgagt
    15001 gtgcctgctg cgtaccggcg tagtgctggc accggatggc ggtattctcg gtaaaatgct
    15061 gccgccgttt cgtcttggcc tgggcgggcc gattggttcc ggtcggcagt atctggcctg
    15121 gattcatatc gatgatatgg tcaacggcat tctctggctg ctggataacg agctgcgcgg
    15181 gccatttaat atggtttcgc cctacccgat acgcaatgaa caatttgccc atgcgctcgg
    15241 tcatgcgctg catcgcccgg ctattttgcg cgtccccgca accgccattc ggctgttaat
    15301 gggcgaatct tcagtactgg tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga
    15361 agcgggtttt gcgtttcgct ggtacgattt agaagaggcg ctggcggatg tcgttcgctg
    15421 atgtggttta ctgcaaacat ccgccagtta actcccggtg ttacagggtt agtggctttg
    15481 cgcgataaga tcgtctggtg aaagtcgggt cagcgtcagg atgacttcgt ggtcaaatcc
    15541 tttttccagc atctcctgag caatacgcag agcttcttcg tgtttgccct gcattgcgcc
    15601 ttcttcacgt aatctgtcag caatggtcat cagtttctcc ttttcttgtg gtgcgcgttc
    15661 tgttatctca ccaataaatg cacggaaacg ctgggcatct cccgtttgta atacataatt
    15721 aaacagggct tttagctgtc tgtcattagt gttccctgta actagcagcg aaacaatttg
    15781 gtcgactaat cccaacagat cgcgctgacg aatatgtttc tgaattaact ccaacagcgc
    15841 cattttacga tgttgcataa tctcgtcatc cggcaccacg gtaatatcca ccaacggaaa
    15901 agccgatgaa tatattttac gggcaatcgc aggctcggca aattcatcaa gccagcagag
    15961 tgaataagga taaggacttc tgcaaccatg ataaaacagc atcgggatca ccaatggaag
    16021 ctctttatag cccacatcaa gatggttttg cattgccgca atggaataac gcatcatgcg
    16081 aaaagccatt aattcttccg gcttactttg gtgctctatc actacataaa gataacccac
    16141 tccctcctgc gttttcacag accacaagag gtcggaataa tattgccgca ggtcttcatc
    16201 aataaaactg tttggttcca gtttaagcgt cgttaaatca cacagtttgc gcagcggcgc
    16261 gggaagatga atatcaataa aatcccgcgc ggtgtctgga tggcgtaaaa aagatttaaa
    16321 taccgcatca tgcggcgtgg aagttgtcga tattgtcatg gcgttccgtc acccctcaaa
    16381 tttaagatga cgcgacaata accacgatta acacccaatg cagcaggcag tttttatctt
    16441 tggacagcga attcagaagt atttttagcg gactgcaact tttacatttc agcggaagct
    16501 gactcgcaaa ttccccttga aaatactccg gcagcgttag ccaccggaga ggcaattatt
    16561 tcagcgaacc tttaaggaac tgttgcagac gagggctttg cgggttgcca aataactgct
    16621 ccggcgcgcc ttcttcctct attttcccct gatggaggaa aatgacgtga gtagatacat
    16681 ggcgagcaaa gcccatttcg tgggtcacca ccaccatcgt ttttccctct tccgccagct
    16741 gctgcataat gcgcagcact tcacccacca gctctgggtc aagggccgat gtcggttcat
    16801 caaacaacag aacttccggt tccattgcca gcgcccgcgc tatggaaaca cgctgttgct
    16861 gaccaccgga caggtgcacc gggtatttcc cctgcgcacg ttcgtctatc ccgacttttg
    16921 ccagatactt caccgcccgc tcgcgcgctt cctgcttgct caggcccaac acctgaatcg
    16981 gcgcttccat gacgttttcc agcaccgtca tatggctcca gagattgaag tgctggaata
    17041 ccatcgtcag gcgtgtgcgc agtaagcgca gttgattttt atcggcgact ttgagttgac
    17101 cgtctttgtc gcgcaccaga ttgatcgtct ggccattgac cacgatcgac ccttcactcg
    17161 gtttttcgag gaagttaatg cagcgcagaa aggtactttt ccccgatccc gacgatccga
    17221 tgatgcttat tacatctccg gcattcgctt gcagtgacac ccctttcagc acttcgtgtt
    17281 cgccatagcg tttgtgcaaa tcgataacgt ttaatttatt ctcggacatc gtattctcag
    17341 tgcgttgaag aaggtttcac atgctgcaac cagcgttttt ccgctctgcg aaagaggctg
    17401 atcaggacat aagagatgat taaatagagc accgcggcaa tgccgaaggc ggtaaaaggt
    17461 tgatacgtgg cggcgttaat atcgcgggct attttcagca gatccggcac cgtggcagta
    17521 aatgccaacg cagtagagtg cagcatcagg atcacttcgt tgctgtatgc cggtaacgca
    17581 atacgcagcg cagaaggcaa aataatgcag cgatacattt taaaagtcga gaagccatag
    17641 gctctggcgg cttcaatttc cccatgcggc accgaacgga ttgccccagc aaaaatctcg
    17701 gtagtgtaag cgcaggtgtt aagcgtcagc gccagcacgg tacagttcag gccactgcgg
    17761 aaaaaggcgt taagaaactc ggtcccctta acaatctcca gcgtatacat gccggaatag
    17821 aacaccagca actgaacata cagcggcgta ccgcgaaaaa tataggtaaa taaccagatt
    17881 ggaaactgga tgtatttatt actggagaca cgaccaatcg ccagaaacag cgccaggact
    17941 ccgcctatca ctaccgacaa aataagcagc cacagagtga tcgccacacc agtaaagcga
    18001 taaccgtcgg tccacagcag cggtttccag tattcatgta agatttcgat cacaggtcag
    18061 ccctcttcac acccacagag tagcggcgct caaggaacag cagcacacca ttggaaacgg
    18121 tggtgaaaac caggtaaatc acgccacaga cgatagcgaa atagaacggt tcccaggtac
    18181 ttttgcctgc cagctgcgtg gctttgacca catcttccag gccgagtaac gaaaccagtg
    18241 cggtagattt gaggatcacc tgccagttgt tgccaatgcc tggcagggcg taacgcatca
    18301 tcgccggaaa catgatccgc cgaaacactt gcccacgagt aaaaccgaac gccctcgccg
    18361 cctctatatg tcctttcggc actgccataa aagcaccacg gaacgtttcg gtaaaataag
    18421 caccgtagat aaaaccgaga gtgataatac cagcgaccat cggatcgata tcaatctgcc
    18481 cgacgcccat cgcctccgtc accgtgttta gcgcaatctg caaaccgtag aaaatcagca
    18541 gcatcaacac taagtccggc acgccacgaa tcagcgtggt gtacccttcg aaaatcagcc
    18601 ccgaaagccg attttgcgag agcttaccgc cagcgccaat taaaccgatg attacagcga
    18661 gcactacaga gctgatagcc agctccagcg tgacgagcgc accctgtaaa ataacacctg
    18721 aaaacccata caacatgctg cctgtcctgt cgtgtgtggt gaattaccgc ctcttgtctc
    18781 cctccggtta cccgaaggga gcgggctttc tttcaccgat ggcgattaac caccataaac
    18841 atcaaaatcg aagtactttt tcgctaattt ctcgtaagta ccgtcagcgc gcatttcggc
    18901 aaaggctttg ttcagtgcct cgcgcagttc gttatcttct ttacgcaggc ccatgccggt
    18961 ccctacgcca aacagttttt catctttaac agacgggcca ccgaatttgt aatctttacc
    19021 gacaggttgt ttgaggaaac cttcgctggc agcgacctca tcctggaacg cggcatcaat
    19081 acgtccggca gtcaggtcag aataaatgtt gtcctgcccc tgatacgaga cgatttcaat
    19141 gccttttggt gcccaatgtt cattaccgaa cgtctcctgg gtagtgccct gcaatacgcc
    19201 tacccgtttg cctttcagcg actcgactgt cggctgaatg tcagaatttt tcgccaccac
    19261 cagacgagaa tcggcagcgt acagtttgtc ggtgaaggct atttcttgct gacgtttttc
    19321 cgtaatggaa agcgatgaca tgatggcgtc aatcttcttc gcttttaagg acgggattaa
    19381 cgcatccagc ggattttcga caaacgtaca ttgcgtattg atgcgtttgc ataattcctt
    19441 tgccagatcg atatcgaagc caaccagttc gccttgtgaa ttttttgatt caaatggcgc
    19501 ataggtcggg tcggtaccga tgcggatgtt ttgcggaatc gcagcaaatg ccgcagttgc
    19561 gctggagaag gccagaacca gagagagcga tagcaccagt tttttcataa tgtcctcaac
    19621 tgacagtctt tttataggga atatttacag gtttgttaca cttatcgtgc cataaaattg
    19681 accatcaagg caaaatatcc cgccccgtta gacgtattat ttttcattaa aaatgcttaa
    19741 gtatgcattt caatgcacca cgatagtgca acaaacctgg cgtgcaccat gatgatcatt
    19801 ccatcaggta cagcttccct gcgacgtatc agtcaccgta gacattaaag tcaaaatact
    19861 ttttcgccat cttgtcgtag gtgccgtcct gacgcagctc gccaagcgcc ttattgaagg
    19921 cagccgtcag ttcagcatca tctttacgta gccctacacc ggtgccatca ccgaagtatt
    19981 ttttgtcttt tactgatgag ccagcaaagg cgaaatcttt accagcaggt tgcttgagga
    20041 atccttcgct ggcagcaact tcatcctgca gtgcagcatc cagacgtcct gcagccagat
    20101 cggaatagac caaatcctgg ttggcatagg ccaccacatc cacacctttg ctacgccagg
    20161 tctcgttagc gtaagcttcc tgggttgatc cctgcagcac accaacatgt ttacctttca
    20221 gtgagtccag cgttggctga atcggcgaac ctttggcggc aatcagacgg gaatctgcgg
    20281 cgtacagctt gtcggagaag gcaatctcct gctgacgttt atcggtaatg gaaagcgacg
    20341 aaataatggc gtcgattttt ttcgctttca gtgaggggat cagcgcgtca aagtcactgg
    20401 caacccaggt acatttcacc tgcatccgtt tgcacatctc gttaccgaga tcgatatcaa
    20461 agccgacaaa gtcacctttg gcatctttcg acgagaacgg tgcataggtg gtatcggttc
    20521 cgatacgtac cgtctccggc aatgccgcat agctggaagc cgctgcggaa ataccgacta
    20581 acaaagacag agcgagaatc gacttcttca tacataaccc tcaagtgaaa atggcttttt
    20641 tatgttgtgt attgtgttgt gtgtttgcag gcttgttcat gcaggtctta tgccatcttg
    20701 acgacaacag taacattcaa cgttaaatat gttaataaga cgttgcatta ttgtcctaaa
    20761 gttgaagata gcaggtatag cggttgaatc gcagcgtttc gattgaatgg cagaaacaaa
    20821 atgtcgagga tttgatcgcg gttgcaaaat tgccctgaaa cagggcaaca gcggagttat
    20881 gcgccctgcc agcgggcaaa gagatcttca ggaagggtta tcgcaaactg gtcaagaaca
    20941 cgattaaccg tctgatttat cacatcatca agggattgcg ggcgatgata aaacgccgga
    21001 acgggaggca taatcaccgc accgatttct gctgcctgcg tcattaaacg cagatggcct
    21061 aagtgcaatg gtgtttcacg cacgcagagc accagtgggc gacgctcttt cagcaccaca
    21121 tctgccgcac gggtcagtaa gccatcagta tagctatgga caatgccgga cagggttttg
    21181 attgaacagg gtaaaatcac catccccagt gtctggaaag aaccggaaga gatgctggcg
    21241 gcaatatcgc gcgcatcatg cgtgacatca gctaatgcct gcacttcgcg cagggaaaaa
    21301 tccgtttcga gggataaggt ctggcgcgcc gcctggctca ttaccagatg cgtttcgata
    21361 tctgtgacat cgcgcagaac ctgtaataag cgcacaccat aaatcgcgcc gctggcaccg
    21421 ctgatgccca caataagtcg tttcataaat agttgccctg tcagacttgc aggcagactt
    21481 tgcaggattt cggcgggagt tgcaagtcag ggtgccagac cggcaccctg agcgaaggca
    21541 tcatccttcg ttatgcattt cgagattttc cacttcgttc tgacgttgca ctgctttggc
    21601 gtcgtcatta cgtaaagtat cgaggaaatc gaggtagccc tgatcaacat ctttggtgac
    21661 gtagacgccg ttgaacaccg agcattcaaa ctgctggata tccgggtttt cagcgcgaac
    21721 ggcttcgatc agatcgttca gatcctggaa aatcaacccg tcagcaccga tgatctggcg
    21781 aatttcatct acttcgcgac cgtgagcgat cagttccgtg gcgctcggca tatcaatacc
    21841 ataaacgttc gggaagcgaa tttccggtgc cgcagaagcg aggtacactt tcttcgctcc
    21901 ggcttcgcgt gccatctcga taatctgctc agaagtggtg ccacggacga tagagtcgtc
    21961 gaccagcagg acgtttttat cgcggaactc ggcgcggttg gcgttcagtt tacggcgcac
    22021 ggacttacga cgcagctgct gacccggcat gatgaaggtg cggccaacat agcggttttt
    22081 aacgaagccc tggcggtacg gtttgcccag aatacgagca atttccagcg cgatatcaca
    22141 cgaggtttcc gggatcggga tcaccacgtc gatatccaga tcttcccatt cgcgggcaat
    22201 tttctcgccc agcttcgtgc ccatattcac acgcgcgctg taaacggaaa ttttgtcgat
    22261 gaacgagtcc gggcgagcaa agtatacata ctcaaacagg cacggattgc tgaccggatt
    22321 gtcagcacat tgacgggtaa acaactgccc ttcttcagtg atgtaaatcg cttcgccagg
    22381 cgcgacgtca cgcaggaaat caaagcccag tgtatcgagc gcaacgcttt cggaagcgac
    22441 catatattct gtgcggttct cgtcaatatc acgttttccc agaaccagcg gacgaatacc
    22501 gtttggatcg cggaaagcaa ccataccgtg gccgataatc atcgccacac aggcatacgc
    22561 gccgcggatt aagcggtttg tagcagcgat ggcggcgaaa atgttgtcgg cttcaagcgg
    22621 gtagtggcgg aagttgtcca gctcgctggc gaagatatta agcagaattt ccgagtcgga
    22681 agtggtgttg atgtggcggc gtttttcttc aaacagtttt ttacgcaact cgtgagcgtt
    22741 ggtcagattg ccgttgtggg caagcgtaat gccatacgga gagttaacgt aaaacggctg
    22801 cgcttcagag gcgctggagc tgccagccgt agggtaacgc acatgaccaa tgcccatatt
    22861 gccctgcaaa cgctgcatat ggcgagcttc aaatacatcg ctcaccagcc cgttcgcttt
    22921 acgcaaacgg aagcagttat tggcatctat ggtgatgatg ccggcggcat cctgaccgcg
    22981 atgctgaagc accgttaagg catcataaat cgactggtta accggcataa caccggcgat
    23041 accgacaata ccgcacatac gtctttttcc tcgttaagcc acatctcaga gcacttacgc
    23101 tctgggcaag aaacttgacg agctttgcag ataatcaaaa aaccatctga tgataaaact
    23161 gaactgcgga atcagttgtg atttactcca gtcttcgctt ttcgacaccc cggtaaagga
    23221 gtcgagaaag aagagaatag cagcaacaat caacacaccg cgcaatgcac cgaaacagac
    23281 gcccagcacc cgatcggtgc ctgacaaccc cgttttctcc accagcaggc ctatcacgaa
    23341 gttcacgata gcgccaacga tcagggtggc gataaacagg accgcgatgg caatcccatt
    23401 tcgaaccagt tcgtcttcaa agcccgtaaa ccagactgac aggtaagtgt agtaatgact
    23461 ggcaacaaag aaagcacaac cccatgtcac cagcgataac gcttcacgaa caaagccgcg
    23521 gatcaggcta accagagagg aaaaagcaat caccgcgatt atggcgtaat caatccagac
    23581 catatgtgtc ccacgatttt acgccctgtc atcctgttcg gggcgcattc taacagaaaa
    23641 agaaaacgtt tgcgtaggga tttccttccc gcgcatcaat aaaaatggcg ctgaaaaaat
    23701 attcaacgcc atcgactttt tatgcctttg cggcatcgga caatgcgtgt cggatgcggc
    23761 gtaaacgtct tatccgacct acggttctac ccctgcgtag gcctgataag acgcaccagc
    23821 gtcgcatcag gcaacggctg tcggatgcgg cgtgaacgcc ttatccgacc tacggttcta
    23881 cccctgcgta ggcctgataa gacgcaccag cgtcgcatca ggcaacgcct gtcggatgcg
    23941 gcgtgaatgc cttatccgac ctacggttct acccctgcgt aggcctgata agacgcgcca
    24001 gcgtcgcatc aggcaacggc tgtcggatgc ggcgtaaacg ccttatccga cctacggttc
    24061 tacccctgcg taggcctgat aagacgcgcc agcgtcgcat caggcaagac cgtattaatt
    24121 cggcgtatag cccattacca cgccacttaa gccagaaagt tgcttcaact cacccagcga
    24181 acctttcagc ttatctttcg aggcatccgg cccaaccaga atacgggtaa ttttaccctg
    24241 cactggcgtg gatggcgacg tataaacccg ataaccggca ccgcgcagct taccgacaat
    24301 ctcattcact ttatcggcat ttttcagcgc gcccagttgc acaacatagg ctttacccgt
    24361 tggcgctgct ttttcttcca cgaccggctt cggttctggc ttcggcgcag gtggtgcttc
    24421 aaccttcggc ttaggcggct ccaccggttt cggcttcggt gggacgaccg gtgcaggttc
    24481 cggttcaaac tcggtgttat tggctgcaat agtggccgga tcgagcgacg gtgctgcggc
    24541 atcacctgcc cgcacctctt ccgctgcgcc ttccggcggt tgcgtcggca acgcctgggt
    24601 ggcggctggc atcatatcag gctcatcgcg atcgcccgct tttggcacca gcgggatagc
    24661 cgcaaactca tcctgataat gttttttctg cccgtccagc agccctggaa gtacaatcac
    24721 ccccagcgcc accagcacga tcgtgcccac taaccgattc tgaaacttac ttgccaccgc
    24781 ttctcctcgc gtcaatcact tccatgacat gtgcgaccgt gtggaacgaa ccacacacca
    24841 gcacggtgtc ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc gcaacgctat
    24901 caaatgattt gccgttaccc aaatgctcaa gcagttgttc tgccgtggca ccgcgcggcc
    24961 cttccagtgg cgcacaatac cagtcatcaa ccacgctttt caaccaggcc agagttccgg
    25021 caatatcttt atcatgtagc ataccgataa ccgccagcac gcgcccgttt ttcggtagcg
    25081 ttttcatacg cccggtgaga tattccgccg catgtggatt atgcgcgaca tcaaaaataa
    25141 cgcgtggcga ctcgctcaca atctggaaac gtcccggcaa aattgcgctg gcaatcccgt
    25201 cgcgaatggc attttcactg acctccagcc cgctggcacg cagtgccgcc agcgccgttg
    25261 cggcattcgg ttgcgggaca agcggcaacg gcaggttttc cagcgtgccg tgagcatcgc
    25321 taaacgccca gtcatgatcg gtgacggaat agttccactc aacgccccga cgttgtaaca
    25381 gtgcaccttt ttcctgcgcc acatcagcaa tggtagaagg catttccggc tcaccgacaa
    25441 ttgccggttt ttcgctgcga aagatgcctg ctttctcgcg accaatactt tcgcgatctg
    25501 gccccagcca gtcggtatga tccagcgcaa tactggttac taccgcgaca tcggcgtcga
    25561 caatattggt tgcgtccaga cgaccgccca gccctacttc cagaatcacc acgtcaagtt
    25621 gtgcctgttt gaacagccac aacgccgaca gcgtaccgta ctcgaaatag gtcagggaaa
    25681 tatcaccgcg tgccgattca atctccgcaa aagaggcggt gtgggccgat tccggcaatt
    25741 cctgaccctg cacacgtacg cgctcggtat aacacaccag atgaggcgaa ctgtagacgc
    25801 ccactttgta ccctgccgcc atcagaatcg actccagcgt acggcaggtg gtgcctttgc
    25861 cattcgtacc cgcaacggta aacacaaatg gcgctggttt cagaacgcca agacgcgccg
    25921 cgacctggct cacgcgctca aggccgagat cgatagtttt actgtgcagg ttttccagat
    25981 aagaaagcca cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata atcatggtat
    26041 ccgctgattc gttacggtga gaatagcaaa agggcagagc cagtggccct gcccttatca
    26101 gttatcaggc ctcaggttcc tgatccggta ccgggggtac cactacgcct tcacgcggcg
    26161 cttcaggatt cggcgctggc agattcatca acttcgccag aatgctcgcc agtttcaggc
    26221 gcatttccgg acgacggacg atcatgtcga tcgcgccttt ctcgatcagg aattcactgc
    26281 gctggaatcc tggcggcagt ttttcgcgaa cggtctgttc gataacacgc ggaccggcaa
    26341 agccgattaa cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg aaacttgcag
    26401 aaacaccgcc catcgtcggg tcggtcagca cggagatgta cggcaagccg cgctcctgca
    26461 tttttgccag tgccgcagag gttttcgcca tctgcatcag cgacatcagt gcttcctgca
    26521 tacgtgcgcc accagaggcg gagaagcaga tcagcgggca gttatcttcc agcgcctgct
    26581 caacggcacg cacgaaacgt gcacccacaa cagaccccat tgaaccgccc ataaaggcga
    26641 actcgaatgc cgcagcgaca accggcattc catacagagt gcctttcatc accaccagcg
    26701 catctttttc gccggtttct ttctgcgcag atgccagacg gtctttatac ttcttggagt
    26761 cacgaaactt cagcacatct ttcggctcaa gctcgctacc cagctccaca aggcttcctt
    26821 catctaacag gctatgcagg cgattacgcg ctgtcatacg catgtgatgg tcacacttcg
    26881 gacagacctc aagattacgt tccagctcag cgcggtataa aacctgaccg cagctatcac
    26941 acttagtcca caccccttca ggaatgctcg ccttgcgggt gggagtaatg ttgcttttaa
    27001 ttcgttcaat ccagctcatt agggaccttt ctgtctgaac ctggttcgat gccagtttta
    27061 tctttgggga cgcataatgc catttttgcc cccaacagac catgaatgtt gcacattaaa
    27121 acataacagc ccgaaacttt ggataaaaaa gtggtcgaac cgcggagtta ctttttattt
    27181 tgcggcgcgt gccgcagcgc gtttgtgacg gattatttcg atgacgcccg gcaaaataga
    27241 aaccacaata atcccgacga tcagcagctt aaggttatcc tgaaccatcg ggattgtacc
    27301 gaagaaatag cctgcgtagg taaaaagcag tacccacaac agtgcgccga tcacgttata
    27361 agcagcgaaa tgacggtacg acatgtggcc cattcccgca acaaacgggg cgaacgttct
    27421 gacgatcggc acaaaacggg cgagaataat cgttttgccg ccatgtttct cataaaactg
    27481 atgggtttta tcgagataac tgcgacggaa aattttcgaa ttgggattac tgaataactt
    27541 ttcaccgaac aagcgcccga tagtgtaatt gaccgcgtca cccacaatcg cggcgatcag
    27601 catcagcacc accatcatat ggacgttgag atcgttggtt tccagtgatg ccaacgctcc
    27661 ggcaacaaac agcaatgaat cacccggtaa aaacggcgtt accaccagac cggtttcaca
    27721 gaacaaaatt aagaacaaaa tggcataaac ccagacgccg tactccgcga ccagctctgc
    27781 cagatgcacg tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt ccatatttgc
    27841 ctatgccttg tactcgtcat ctttcaggct gtaactacgt tggctacagc ctgaaatgtt
    27901 ccgagcatta ttctcgttag tccgccagaa atagcgggcc cattggcggt tttggaagat
    27961 cataccggtc agggtaatcc accgcgacca gatacagccc ttccgctttt gccgttgctg
    28021 ccgccagcgt tctgtccttt gccgccagca actctgctat ccagctctcc ggctggttgt
    28081 gggcaccgac ttccatcagg ctgccgacaa tattcctgac catatgatgt acaaaggcat
    28141 tcgctttgat atcaaccacc acataaggac cgtgacgcgt gacattaata tgcataacgt
    28201 tgcgccacgg ggttcgggac tggcactgca ccgcacggaa cgaggtgaaa tcattctcgc
    28261 ccagcaagca ctgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg taaaaatggg
    28321 ttaccccttt actcagtacc gccgggcgca gccgatgatt gtagatgatg tagcgataac
    28381 ggcgagccgt ggcgctaaat cgggcatgaa aatcatcagg tacagcttta acccaacgca
    28441 cggcgatgtc accaggtaaa ttcgcattta cgcccaacgt ccacgcagcg tctttgcgct
    28501 gcgcggtagt ttcgaaatgc acaacctgcc cggtaccgtg tacccctgcg tcagtacgcc
    28561 cggcgcagaa gacggtgatg ggttcgttcg ccacctggga gagcgccttt tccagcttct
    28621 cctgcacact gcggacttca ttctgccgtt gccagccgta atacttactg ccgtcgtact
    28681 caatgcccag cgcaatttta taaactggcg gttgttgctg gtcggacatt agtacagata
    28741 ctcctgcacc agtttctcgg cgatttttac tgccatcagc gcgccgccaa agcgaacgtt
    28801 atcggccacc gaccagaact ggacttgctc cggcataccg tagtcattac gcacgcagcc
    28861 aacagaaaga tgcggcgtac ccgaagcatc acctacctga gttgggaatt cgttctcttc
    28921 agagagcaca atatcttccc cttgaacaaa cgcatcacgc gcttcttctg ctgccagtgg
    28981 acgcagagct tcaaagttga ccatctgggc atgaccgtag aataccggtg cctggacgac
    29041 gcttgccgaa atcatcagtc cttcgtcctg caggattttg cgtacttcgt caacgatacg
    29101 acgttcttca cgcacgctac cttcgctatc cggcagtaac ggcagcatgt tgaacgcgag
    29161 ctgacgcccg aagaaatctt cttcgtcaat cggaatgccg ttgagcaatt tcgcactctg
    29221 ccccgctaac gcatcgaccg cttttttgcc ctgggcggag gctgaaatca ggctggtaac
    29281 actgatacgc gacaaaccgc cctgatcgat taacggtttc agtgccgcca gcagctggct
    29341 ggtcagactg tctggtacgg cgatgacatt ccggttgcgg taatcggtca gtacaaacgg
    29401 gtttacttcc ggcaccacca gcggtacgtc gggttcaaga gcaaacaatc cgctgctgtc
    29461 gatcaccagg caacctgagt tggtcgcttc ttcaacccag gctgcggtag cttctttgcc
    29521 tgcgacaaaa aatgccagct gcgcctgcgt ccagtcgaat tcagcagcat cctgcacggt
    29581 gattgtctta ccaccaaagc gcaattgttc gcctgcgctt tcgttacgtg ccagtgcata
    29641 aatttcccca accgggaact gacgttcagc cagcgtttca agcagggctt cgcccacagc
    29701 gccagttgtg cccaggacgg caatgttcca gccttcagac atggtggttt actccagaaa
    29761 tagcaaagct ccctgccaat gttatagcag agagcatgaa gaagagatta acgtgccgga
    29821 tgatgaacgg cgttaaaacc cagtttacac agcaatgatg ccgcactggc gtcatcacaa
    29881 attacataca gagatgacca ttcacggcgc tcaagatagt ttttgcgcag tttatcgaac
    29941 tcacccggta tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac atcatacacc
    30001 aaatgcacca gccttttcag cgtcggttga tcgagcggtc catgcagagt aatgcgacca
    30061 aactctggcg caggcagtaa tgtatccagc gcaacgtgct gttcatgccc aataaacttg
    30121 ctataggctt caaacacttg cgtagtaccg cgtgctttac cttccagggt atagcctgca
    30181 atatgcggcg tgccgatatc cacttttgtc agcaattcca catttagttc cggttcgcct
    30241 tcccagacat ccagcactac gcttaacttc tggccttcat tcaggcaggt cagcagcgcg
    30301 gtattatcga cgactgcgcc acggcaggcg ttaatcagaa tcgctccggg cttcaggcta
    30361 cggatcagtt tttcatccgc cagatgcagc gttttgtacg gcccgtcttt gaagagtggc
    30421 gtatggaaag tcagaatatc cgcgcgctgg actaactcat ccagcgagcg gaaatcaccc
    30481 tcatccccac agtcggcgcg aggcggatcg caaagtaagg ttttaatccc taacgcttcc
    30541 agtcgtgcct gtaaacgccg accaacgtta ccaacgccta cgatccccac ggtacgctcg
    30601 tgcagtgaaa atccatcgcg ttcggcaagc atcagcaggg aggagaaaac atattccacc
    30661 accgcaatcg cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg cttcaaccat
    30721 gcttcatcga catggtcggt ccccgcagtg gcagtgccaa caaatttaat gggttttcct
    30781 gccagcaaag attcattcac tttcgtgacc gaacgcacca tcagcgcatc cgcgtctgcc
    30841 agttgagcga cggggattgg acgcccggga accgcgatca cctcacccaa acggctaaat
    30901 aagtcgcggg cataaggcat attttcatca acaaggattt tcacgtttgt gttacctgta
    30961 tgagacgaga gttaaccgga caagtgtgcc ataatctcgc ggccaggcat acttgcgaag
    31021 atttcaggta taaggatacg taatgataca acctatttcc ggccctcctc ctgggcaacc
    31081 accaggtcag ggagataacc tgccgtctgg cgcgggcaat cagcctttat ccagtcagca
    31141 acgtacttcg ctggaaagct taatgacgaa agtgacctca ctgacgcaac agcaaagagc
    31201 agaactgtgg gcgggtatca ggcacgatat tggtctgtcg ggagattcac cgctgctttc
    31261 gcgtcactgc cctgccgctg agcataatct ggcgcaacgt ctgctggccg cgcaaaaaag
    31321 ccattctgcc cgccagcttt tagcgcaatt aggggagtat ttacgtctgg ggaataatcg
    31381 tcaggcggtc acggattata tccgtcataa ctttggtcag acgccgctga atcagctctc
    31441 accggagcaa ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg ttattccgca
    31501 accgcagcag agcgaggcga ccgatcgtcc tctattaccg gcggagcaca atgcgctcaa
    31561 acagctggtg accaaacttg cggcggcaac gggggagccc agcaaacaga tctggcaatc
    31621 gatgctggaa ctttccgggg tgaaagatgg cgagttaatt ccagcgaaac tgtttaacca
    31681 tctggtgacc tggctgcagg cgcgtcagac gctaagccag caaaatacgc cgacgctgga
    31741 atcactacaa atgacgctaa aacaaccttt agatgccagt gaactggcgg cgttatcggc
    31801 atatatccag caaaaatatg gcctttctgc gcaatcatcg ctttcttctg cccaggccga
    31861 ggatattctt aatcagcttt atcaacggcg ggttaaaggg attgatccgc gtgatatgca
    31921 accgctgctt aatccttttc caccgatgat ggacacgttg caaaatatgg caacgcgtcc
    31981 cgcgctgtgg atactgttag tcgcgattat cctgatgctg gtctggctgg ttcgttaacc
    32041 ccgacgaaac gacagtatcg tgacaataat tcccagcacc gcagagatcg ccccggcaag
    32101 aaataccgaa gagtaaccaa acgtggtcgc cagcattccc gcaagcggcc cggagacgcc
    32161 gagggcgata tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa cttgtgaggg
    32221 gacgcgttta accacctcca cacccagcgc aggaaagata agcgaacatc cggctccggt
    32281 taacgccgcg cccgctaatg cgacccacgc ccctggggct tgccagagca gcaacaagcc
    32341 caccgtttct acaagcagag agacaatcgc cactttcacg ccgccaaaac ggtccggcat
    32401 ccagccaaac atgacgcgca tcacgacaaa tgcgccgcca aacgcggtaa gagtaaagcc
    32461 cgccatcgcc catcctttgc tggcaaagta gagcgaaacg aaagtcccga taaccgcaaa
    32521 accaacgcct tgtagtgcca gacctaaccc tggtttccag ataagtccga caacgctcca
    32581 cagagatgga cgttctcccg ccagggctgg tactttgcgc actgtgccgt tacaggccca
    32641 cgccagtaag ggtaatgcca ttgtggtgag cgccagtgcg gcaaaaccgt aatggctatg
    32701 aatcaacagg ccaagcggag caccaacagc gagggcaccg taaatcgcca ttccattcca
    32761 tgacatcact ttgccagagt gttttggccc tacgatgcct aacccccagg tcagagcgcc
    32821 tgtcagtaac tggctttcac caaacccaag aatcaaacgc ccgatgacca acagggcaaa
    32881 tttgaacggt gcggagacag gcaaaatcgc cgccagcagc aacgcgccgc cagccagacc
    32941 acaagctaac atcccctgaa gcgccgaacg ttttgcgcca tattgatcgg ccagtcgccc
    33001 ggcatagcca cgcgtcagca ccgtagccag aaactgaatg ccgacggcaa tgccgaccat
    33061 ggtattgcca tagcccagat catgatgaac aaacaacggg ataaccggca acggcaaccc
    33121 tacggtcatg taggtgagaa aaaccgcaaa agcgatgcgg aagagcgaaa aattggcaga
    33181 agatcgtgtt tcggtttggc ttacagcagt catgcattac tccagaatgc agcgcaaggc
    33241 gaggagtatc cccgtctcat ctctctggtt tcagggttac agtgcgttgg caggatttaa
    33301 cgcgtacgtc ttttcagaag gaaatcgaca aagcggggag tttgcctgga actggcggcg
    33361 attgtcaatg atgtgaaaaa gggaaccatc aggttccctt ttgcgttagt gccggaggcc
    33421 gcattggcgc gtaacgtcgg atgcgacgct ggcgcgtctt atccgaccta ctccggccta
    33481 ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg ccgaagccga
    33541 agctgttaga cataacagtg gtcagttcgc gatcggtcgt ttcggtcacg atgttcagac
    33601 ccgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag ccgtgttcca
    33661 gcatcagcag agagtagata gcttcctgta cgccagcagc gcccagagag tgaccggtca
    33721 tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg atagctgcca
    33781 gctctttcac gtcgccaacc ggagtcgaag tgccgtggga gttcaggtaa tcgattgggg
    33841 tatcaacgcc atgcatcgcc atctgcatac aacgtactgc gccttcgcca gagggagcaa
    33901 ccatgtctgc gccatcagac gttgcgccgt agccaacgat ttcagcatag atgtgagcac
    33961 cacgcgccag cgcgtgttcc agctcttcaa ccaccaccat accgccgccg ccagcgataa
    34021 cgaaaccgtc acggtgagca tcgtaagtac gggaggcttt ttccggggtg tcgttgtatt
    34081 tagtagacag cgcacccatt gcgtcgaatt cgcaagccat ttcccagcac agctcttcgc
    34141 cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact gcgttaccga
    34201 tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca tgaattttaa
    34261 acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc acatacgggc
    34321 caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaacacc tggaaacgcg
    34381 gggaaccgcc gccagaacct gcaatcaggc caacacgcgg attattctgg taagcttccg
    34441 gagagaggcc cgcatctgcg attgcctgct ccatagaaag gaatgcataa atggatgcgt
    34501 cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt tttacgttgc
    34561 cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg atccctgaac
    34621 gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg gaaacaatgc
    34681 ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac atagagtaag
    34741 tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc caattaatag
    34801 agaaatttgc gcagccttac acacatcgct aagatcgagc taccgcctgt aagacgagta
    34861 acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc tgagggtaca
    34921 cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct ggaagagacg
    34981 cgttatgttt ttctgggagg taaccaatta gaggcacgct ttcctgaaca tccacatcct
    35041 ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac gctatggcag
    35101 gcatttgatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt acatttcatt
    35161 agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca acactggccg
    35221 gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt gcccggttgc
    35281 catcgtttat tacttgatga aggccgcgtg acgctggatt tatggtttgg cgatattaat
    35341 gaactgatca gccaactgga cgattctcta aatcaaaaag tagatgcctg gtttctggac
    35401 ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa atctgtttaa cgccatggca
    35461 aggttggcgc gtccaggcgg cacgctggcg acatttacgt ctgccggttt tgtccgccgc
    35521 ggtttgcagg aagccggatt cacgatgcaa aaacgtaagg gctttggacg caaacgggaa
    35581 atgctttgtg gggtgatgga acagacatta ccactcccct gctccacgcc gtggtttaat
    35641 cgtacgggca gcagcaaacg ggaagtagcg attatcggcg gtggtattgc cagcgcattg
    35701 ttgtcgctgg cgctattgcg tcgcggctgg caagtaacgc tttattgcgc ggatgaagcc
    35761 cccgcgctgg gtgcttccgg caatcgccag ggggcgttgt atccgttatt aagcaaacac
    35821 gatgaggcgc taaaccgctt tttctctaat gggtttactt ttgctcgtcg actttatgat
    35881 tcattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg ctgggatgag
    35941 aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgaaga actggctgta
    36001 gccgttgagg caaatgcggt tgaacaaatt acgggcgtta cgacaaattg cagcggtatt
    36061 acttatccgc aaggtggctg gctgtgtccg gcagaactga cccgtaatgt gctggaactg
    36121 gcgcaacagc agggtttgca gatttattat caatatcagt tacaggattt atcccgtaag
    36181 gatgactgtt ggttgttgac gtttgcagga gatcagcaag cgacacacag tgtagtggta
    36241 ctggcgaacg ggcatcaaat cagccgtttc agccaaacat cgtctctgcc agtgtattcg
    36301 gttgccggtc aggtcagcca tattccgacg acacccgaac tggcaaaact gaaacaggtg
    36361 ctgtgctatg acggttatct cacgccgcaa aatccggcga atcagcacca ttgtattggt
    36421 gccagttatc atcgcggcag cgaagagacg gcgtacagtg aagacaatca gcagcagaat
    36481 cgccagcgat tgattgattg tttcccacac gcacaatggg cgaagacggt tgatgtcagt
    36541 aaaaaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc aatggtaggt
    36601 aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgctggcgga gcagaaagat
    36661 aaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgctgcttt aggttcgcgc
    36721 ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag cgacgaaccg
    36781 attccgatgg atgccagtac gctggcggcg ttaaatccga atcggttatg ggtgcggaaa
    36841 ttgttgaaag gtaaagcggt taaggccggg taatctgctc tcgcagtgtt tgccggatgc
    36901 ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac gcgataagcg
    36961 tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct gctgaaacag
    37021 gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac cagcaccaat
    37081 ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt ggtcatcacc
    37141 actttccagt tctgcgatgg ctaatgtaag gtggccacgc aaatacccac tggcaaacaa
    37201 ctcatcatca ctggcatggt caaccatacc gtcgattaat gccagaatgc gtgattcaaa
    37261 ctccgcgatc atcttctttc ctcattgtaa aacgtggcgc tggcttcagt tgagcgcttc
    37321 cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt gtagtgcttt
    37381 aatgaaacgt gccgggcgtt ccggaatgcc atttgttaga taatccatca cctgcgcatg
    37441 tactcgccgt tgaaacacca cgcggtccgg ttcgaagtcg ccttccagat tgtcgcaact
    37501 gacgttaaac gggtatcccg ccgccacgca gaacaaccag tcgagcgcct gcggcttcac
    37561 ttcaacatct tcaaactggc tttgcgtttg ggcatcgcga ccgtccgggc aataccagta
    37621 gccgaaatca acctgctcgc ggcgcgcttt cccggcaata caccagtgcg aaatctcatg
    37681 aattgcgctg gcataaaagc cgtgggcaaa gacgattcgg ttatacggta cttccgcatc
    37741 agcgggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa aatcatcggc
    37801 aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt tcattagttc
    37861 atccccaacc agtggaggat ctcctgtccg tggctatcat aaagtaattt ggcactcatc
    37921 accgccgaga cgataacgat catcgggcgg atcagttttt gtcctttgct caacaccagt
    37981 cttgaaccca tgcgcgcgcc aaggaactgc cccaccagca tcacaaagcc cgtcgcccaa
    38041 atcactttgc cgccgagaat aaacagcagt aaaccgccga tgtttgacgt tgcgttgagt
    38101 aatttggcgt gagccgtggc tttggcgagg ttgaatccgc acagcgtaac aaaggccagc
    38161 gcgtaaaacg atccggctgc cggaccaaag aatccgtcat aaaaaccgac gcagccaccg
    38221 gcaattaacg cgaagggcaa accgtacatc cggcgctggc gatcttcttc acccagcttt
    38281 ggcatcagca agaaatagag gccgatacaa atcaccagaa tgggcaaaat ctgccgtaag
    38341 acatcagcct gaacgtactg taccaacagt gcgccgctca ttgagccgac aaaggtcatg
    38401 gcaatattaa gtttctgatc gcttaaacta accactttgc ggcgaataaa gtagatagtg
    38461 gcggaaatag agccgccgca ggcttgcagt ttattggttg ccagagcatt agcgggagac
    38521 atccccgctg ccatcaatgc cggaatggtg agtaacccac cgccaccggc aatcgagtcg
    38581 ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg ggaaaccata
    38641 aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc tggcaggaag
    38701 gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt ggaggttcaa
    38761 accagctttg cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt tgatcttcac
    38821 actccagact atcggcagga caacgtaatc gtacatgcat atgcgcacga tgctggaacc
    38881 agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atcaaggcaa agttgttgtt
    38941 taatcgccgg attaacaaaa atgcgcgtga cgtctttgtc ctgggcggcg agtttgatca
    39001 agctgaaaat ttctggcttc cacagcgtgg agacaacgtg tttaccgtcg cgggaaacta
    39061 agtccagtgc ttgcgggcgc aagagctgcg cggaggtccg gcgtgttttc ggcagttgca
    39121 gaaagatatc gacatccagt ccggtctggt ggctggcgtg accgccgttg aaacgcccac
    39181 cagcaggcat tcccatatcg ccaatcaaca ccgtacccat gcccagattg ctcacctgac
    39241 tactcaggcg ctggataaac atcaccaggt ccgggtgacc gaaatagcga cgctgatcgg
    39301 tacgcatgac ctgataatgt tcggactgta tcggcaacgt gtcagcgccg acgatacaac
    39361 cattggaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt attttttgcc
    39421 acggcgtcgc tgccaggctg acgctactgg caagcagggc cagcagcgca atcgcggttt
    39481 tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc gcccgctgcc
    39541 gtaacaggtg atccattaaa acgatcgcca gcatcgcttc tgctatcggc accgcgcgga
    39601 tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg ccaaagcggt
    39661 taatggtgcg ccccggcacg gtaatactgg aggtcggttt cagtgccata tgagcaatga
    39721 tttgctgccc gctgctaatt ccgccgagaa tgccgcccgc atggttactc tggaaaccgt
    39781 ctttggtgat ttcgtcgcgg ttctggctgc cacgcagcgc caccacgtca aaaccatcac
    39841 caatttccac gcctttcacc gcgttaatgc tcattagcgc atgggcgatg tcggcatcca
    39901 ggcgatcaaa gaccggctca ccaagcccgg caggaacgcc actggcaaca acggtgactt
    39961 tcgcgccaat agagtcgccc tcttttttca gcgcgcgcat cagttcatct aacgcgtcga
    40021 ttttgtccgg gtccgggcag aagaatggat tttgctcgac ctgcgaccag tctttgattt
    40081 ccagcggaat gtcgcccatc tgggtcaggc agccgcgaat ttcgatgcca aatttctcag
    40141 cgagatattt tttggcaatc gcccctgctg caacgcgcat ggcggtttca cgggcggaag
    40201 aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg taatcggcat
    40261 ggccaggacg gaaaacgtcc ttaatcgcac tataatcctg agaacgctga tcggtatttt
    40321 cgatcaacaa accaatgctg gtgccggtag taacaccttc aaaaacaccg gagagaattt
    40381 tgacctgatc tggctcacga cgctgggtgg tatagcgcga tgtcccaggg cgacgacggt
    40441 cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca ccatcgacga
    40501 tgcagccgag cgccagcccg tgcgattcgc cgaaggtggt tacgcgaaag agttgtccaa
    40561 ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac ttaatcttta
    40621 taaatcgcga aatgttcacg ggccgccagc agctgcgctt tggtgagcat aaacacacca
    40681 tcgccgccgt tatcaaactc cagccaggtg aacggaacat cgggatattg ttccataaga
    40741 tgtaccatgc tgttgccgac ttcacaaatc agtacgccat catcagcaag ataatctgcc
    40801 gcgtttccga gaatgcgacg cgtcagtttc aggccgtcgg tgccagatgc caggcccagt
    40861 tccggctcgt ggcggtattc atttggcagg tcggacatat cttccgcatc gacatacggc
    40921 gggttagtga caatcaggtc gtactgcact ttcggcaaat cacggaacag atcggaacga
    40981 atcggtatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc aaccgccagc
    41041 gcgtcgggag agatatctac cgcatcgact tccgcttccg ggaaggcata agcacaggca
    41101 atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg cttgctgata
    41161 agtccggcaa atttattgtt gatcaattca ccaatcggcg agcgcggcac cagcacgcgt
    41221 tcatcgacgt aaaattcatg gccgcagaac cacgctttgt tggtcaggta agctaccgga
    41281 atgcgttcgt tgacgcggcg gatcacgcgc tcaacaatac gatgtttttc gctggaggtc
    41341 agacgcgcgg tgcgcatatc ttccggaata tccagcggca ggtagagcga cggcaatacc
    41401 agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat atttgccgcg
    41461 ctgaagcggc tcaccgacca gcgcaacatg tcctgaatgg tttgcagctc atttactgct
    41521 tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata atttcggcgg
    41581 ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg aggaaaaata
    41641 cgtttaaaac gatcgattgc gctacgagtc gggtaaactg taggaaaatt agaaatagag
    41701 acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg tttcgccagt
    41761 tgatggcggg gactcgcaag attaagcagg acaccattgt ccatcggcca cagcgtaaaa
    41821 aagtcagcga agtgcccgtc aaacggttga ttcaggagca ggttgacgcc agtcactatt
    41881 tctctgacga atttcaaccg ttattaaatg ctgatggccc ggtgaagtat gtacgccctg
    41941 gtgtagacca ttttgaggcg aaaaagctgc gccgcggcga ttattcgccg gaattgtttt
    42001 tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg ttgatcgccg
    42061 cctgtcgccg cgaacatgtg ttttgcgcct gcgtgatgca tggtcacggg aaacatatat
    42121 tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtcatggcg tttcaccagg
    42181 caccaaaaga gtatggtggt gatgccgcat tgctggtgtt gattgaagtg gaagagtggc
    42241 taccgccgga gttgccatga atattagggg gcggatgcgt tttgccattt cgccctgaat
    42301 gctggtatcg aatgccggat gcggcgtaaa cgcctgatcc ggcttatcaa aatgtgcaaa
    42361 ttcaatacgt tgtgattcag ttgtaggcat gataagacgc gtcagcgtcg catcaggcat
    42421 caacgcacaa ctacagttca ttcagtacat actggaaacg gcatgtagaa tcaattggct
    42481 cacggctact ggtattcacc tgtaccacaa tgtcgtgtag ctttctatcc gtcaatggtt
    42541 gacgtgaggg ggtgactgtc acctttgcat tatcacaacc atacgaggta aaggttaata
    42601 agtcagaaac agcacgacaa tatgcccctc tggaatcaaa aatactgcca tcgcagagtt
    42661 tgccgctttc catcaggtag gtcgaaagcg tagtcgtgat tgtcgaacca tcggattgca
    42721 atcgcagcgc tttattatgg ctaacataat tatcactcca ggatatatca aacgaagcga
    42781 cgggaattgt cgggcaaata gaatcatgaa ctgttgggct tgtatacgta ccaaatccaa
    42841 atgcctcaga tactgaataa aatgaggcaa tccacggaca accataatta tcagattcta
    42901 cagtgacgtt aaaagtcata tatgcacaga caaagacacg agtattgata ccagggcaat
    42961 ttgaggtgtc cgtacgtcta taatcagtca aaaccagtgt cggtgccggg ccagatccgc
    43021 tccaggatat taacttgagg gtgtcgcctt ttcttgcatt ggagttgtat tgtccgtagc
    43081 ttacttctcc cggcgtcact accgtgacat agaaatataa actgccggaa gcagtaacgt
    43141 ttttcatcgc ataataaatt ttagtactgg aaaagcttgc actcatgagt ttatcaccgg
    43201 catagcactt tggtgtttct aaactgagag caagtaatat cagacttgtc aaaaatatcg
    43261 tccatttttt cattttaaaa atactcaaaa gttaccgtca ttgtggcggt gaaatcaccc
    43321 gatgtaacat ttcgtccatt tacggtctgt aaccaggcat taaaattgac actgttattt
    43381 ccctgattta atggaaatga ggcacctgtg gtactgttaa taggtacagc cgcccctccg
    43441 gcagtttcaa tgccaatccc aacacccgtt gcagaagatg acgaatcaat cgacagaaat
    43501 cctggtaagt cggtatcttc tgttcccatc aaggtcacct tgacattaaa cgccgtggtg
    43561 cttttgcaat ctttcaactg aaaaactaac ggtacgcgat ctgactgacc acgttgcatt
    43621 aaatcgctgg cagcaattgt ggggaaatga atttctgcaa gtaagttgc
//
LOCUS       DABSYT020000027        43220 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.027, whole genome shotgun sequence.
ACCESSION   DABSYT020000027 DABSYT020000000
VERSION     DABSYT020000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 43220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.027"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(157..369)
                     /gene="rpmE"
                     /locus_tag="ID725_RS09500"
                     /old_locus_tag="pgaptmp_001900"
     CDS             complement(157..369)
                     /gene="rpmE"
                     /locus_tag="ID725_RS09500"
                     /old_locus_tag="pgaptmp_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGV7850512.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            572..2770
                     /gene="priA"
                     /locus_tag="ID725_RS09505"
                     /old_locus_tag="pgaptmp_001901"
     CDS             572..2770
                     /gene="priA"
                     /locus_tag="ID725_RS09505"
                     /old_locus_tag="pgaptmp_001901"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGV7850513.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2926..3951
                     /gene="cytR"
                     /locus_tag="ID725_RS09510"
                     /old_locus_tag="pgaptmp_001902"
     CDS             2926..3951
                     /gene="cytR"
                     /locus_tag="ID725_RS09510"
                     /old_locus_tag="pgaptmp_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGV7850514.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4043..5002
                     /gene="ftsN"
                     /locus_tag="ID725_RS09515"
                     /old_locus_tag="pgaptmp_001903"
     CDS             4043..5002
                     /gene="ftsN"
                     /locus_tag="ID725_RS09515"
                     /old_locus_tag="pgaptmp_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGV7850515.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5095..5625
                     /gene="hslV"
                     /locus_tag="ID725_RS09520"
                     /old_locus_tag="pgaptmp_001904"
     CDS             5095..5625
                     /gene="hslV"
                     /locus_tag="ID725_RS09520"
                     /old_locus_tag="pgaptmp_001904"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGV7850516.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5635..6966
                     /gene="hslU"
                     /locus_tag="ID725_RS09525"
                     /old_locus_tag="pgaptmp_001905"
     CDS             5635..6966
                     /gene="hslU"
                     /locus_tag="ID725_RS09525"
                     /old_locus_tag="pgaptmp_001905"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGV7850517.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7033..7959
                     /gene="menA"
                     /locus_tag="ID725_RS09530"
                     /old_locus_tag="pgaptmp_001906"
     CDS             7033..7959
                     /gene="menA"
                     /locus_tag="ID725_RS09530"
                     /old_locus_tag="pgaptmp_001906"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGV7850518.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8052..8537
                     /gene="rraA"
                     /locus_tag="ID725_RS09535"
                     /old_locus_tag="pgaptmp_001907"
     CDS             8052..8537
                     /gene="rraA"
                     /locus_tag="ID725_RS09535"
                     /old_locus_tag="pgaptmp_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGV7850519.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            8597..9271
                     /locus_tag="ID725_RS09540"
                     /old_locus_tag="pgaptmp_001908"
     CDS             8597..9271
                     /locus_tag="ID725_RS09540"
                     /old_locus_tag="pgaptmp_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850520.1"
                     /translation="MQNIILLFIYLLLIAVNHFRYKFLLSGNAGEMILYFANVFFNPL
                     ALSFIIATIICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGIT
                     VTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHV
                     CDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKM
                     MLSARQ"
     gene            9394..10020
                     /locus_tag="ID725_RS09545"
                     /old_locus_tag="pgaptmp_001909"
     CDS             9394..10020
                     /locus_tag="ID725_RS09545"
                     /old_locus_tag="pgaptmp_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850521.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(10059..10304)
                     /gene="zapB"
                     /locus_tag="ID725_RS09550"
                     /old_locus_tag="pgaptmp_001910"
     CDS             complement(10059..10304)
                     /gene="zapB"
                     /locus_tag="ID725_RS09550"
                     /old_locus_tag="pgaptmp_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGV7850522.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            10730..11575
                     /gene="glpF"
                     /locus_tag="ID725_RS09555"
                     /old_locus_tag="pgaptmp_001911"
     CDS             10730..11575
                     /gene="glpF"
                     /locus_tag="ID725_RS09555"
                     /old_locus_tag="pgaptmp_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGV7850523.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            11598..13106
                     /gene="glpK"
                     /locus_tag="ID725_RS09560"
                     /old_locus_tag="pgaptmp_001912"
     CDS             11598..13106
                     /gene="glpK"
                     /locus_tag="ID725_RS09560"
                     /old_locus_tag="pgaptmp_001912"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGV7850524.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            13336..14346
                     /gene="glpX"
                     /locus_tag="ID725_RS09565"
                     /old_locus_tag="pgaptmp_001913"
     CDS             13336..14346
                     /gene="glpX"
                     /locus_tag="ID725_RS09565"
                     /old_locus_tag="pgaptmp_001913"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGV7850525.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            14443..15189
                     /gene="fpr"
                     /locus_tag="ID725_RS09570"
                     /old_locus_tag="pgaptmp_001914"
     CDS             14443..15189
                     /gene="fpr"
                     /locus_tag="ID725_RS09570"
                     /old_locus_tag="pgaptmp_001914"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGV7850526.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(15194..15622)
                     /gene="uspD"
                     /locus_tag="ID725_RS09575"
                     /old_locus_tag="pgaptmp_001915"
     CDS             complement(15194..15622)
                     /gene="uspD"
                     /locus_tag="ID725_RS09575"
                     /old_locus_tag="pgaptmp_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGV7850527.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(15649..15948)
                     /gene="yiiS"
                     /locus_tag="ID725_RS09580"
                     /old_locus_tag="pgaptmp_001916"
     CDS             complement(15649..15948)
                     /gene="yiiS"
                     /locus_tag="ID725_RS09580"
                     /old_locus_tag="pgaptmp_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGV7850528.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(16160..16600)
                     /gene="yiiR"
                     /locus_tag="ID725_RS09585"
                     /old_locus_tag="pgaptmp_001917"
     CDS             complement(16160..16600)
                     /gene="yiiR"
                     /locus_tag="ID725_RS09585"
                     /old_locus_tag="pgaptmp_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGV7850529.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            16701..17300
                     /gene="yiiQ"
                     /locus_tag="ID725_RS09590"
                     /old_locus_tag="pgaptmp_001918"
     CDS             16701..17300
                     /gene="yiiQ"
                     /locus_tag="ID725_RS09590"
                     /old_locus_tag="pgaptmp_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGV7850530.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            17408..18175
                     /gene="tpiA"
                     /locus_tag="ID725_RS09595"
                     /old_locus_tag="pgaptmp_001919"
     CDS             17408..18175
                     /gene="tpiA"
                     /locus_tag="ID725_RS09595"
                     /old_locus_tag="pgaptmp_001919"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGV7850531.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(18230..18985)
                     /gene="cdh"
                     /locus_tag="ID725_RS09600"
                     /old_locus_tag="pgaptmp_001920"
     CDS             complement(18230..18985)
                     /gene="cdh"
                     /locus_tag="ID725_RS09600"
                     /old_locus_tag="pgaptmp_001920"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGV7850532.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(19092..20081)
                     /gene="sbp"
                     /locus_tag="ID725_RS09605"
                     /old_locus_tag="pgaptmp_001921"
     CDS             complement(19092..20081)
                     /gene="sbp"
                     /locus_tag="ID725_RS09605"
                     /old_locus_tag="pgaptmp_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGV7850533.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(20400..21362)
                     /gene="pfkA"
                     /locus_tag="ID725_RS09610"
                     /old_locus_tag="pgaptmp_001922"
     CDS             complement(20400..21362)
                     /gene="pfkA"
                     /locus_tag="ID725_RS09610"
                     /old_locus_tag="pgaptmp_001922"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGV7850534.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(21543..22445)
                     /gene="fieF"
                     /locus_tag="ID725_RS09615"
                     /old_locus_tag="pgaptmp_001923"
     CDS             complement(21543..22445)
                     /gene="fieF"
                     /locus_tag="ID725_RS09615"
                     /old_locus_tag="pgaptmp_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGV7850535.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(22594..23094)
                     /gene="cpxP"
                     /locus_tag="ID725_RS09620"
                     /old_locus_tag="pgaptmp_001924"
     CDS             complement(22594..23094)
                     /gene="cpxP"
                     /locus_tag="ID725_RS09620"
                     /old_locus_tag="pgaptmp_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGV7850536.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            23244..23942
                     /gene="cpxR"
                     /locus_tag="ID725_RS09625"
                     /old_locus_tag="pgaptmp_001925"
     CDS             23244..23942
                     /gene="cpxR"
                     /locus_tag="ID725_RS09625"
                     /old_locus_tag="pgaptmp_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGV7850537.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23939..25312
                     /gene="cpxA"
                     /locus_tag="ID725_RS09630"
                     /old_locus_tag="pgaptmp_001926"
     CDS             23939..25312
                     /gene="cpxA"
                     /locus_tag="ID725_RS09630"
                     /old_locus_tag="pgaptmp_001926"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGV7850538.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(25418..26092)
                     /gene="yiiM"
                     /locus_tag="ID725_RS09635"
                     /old_locus_tag="pgaptmp_001927"
     CDS             complement(25418..26092)
                     /gene="yiiM"
                     /locus_tag="ID725_RS09635"
                     /old_locus_tag="pgaptmp_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGV7850539.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(26241..27224)
                     /gene="kdgT"
                     /locus_tag="ID725_RS09640"
                     /old_locus_tag="pgaptmp_001928"
     CDS             complement(26241..27224)
                     /gene="kdgT"
                     /locus_tag="ID725_RS09640"
                     /old_locus_tag="pgaptmp_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGV7850540.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(27484..28104)
                     /gene="sodA"
                     /locus_tag="ID725_RS09645"
                     /old_locus_tag="pgaptmp_001929"
     CDS             complement(27484..28104)
                     /gene="sodA"
                     /locus_tag="ID725_RS09645"
                     /old_locus_tag="pgaptmp_001929"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGV7850541.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            28389..29423
                     /gene="rhaT"
                     /locus_tag="ID725_RS09650"
                     /old_locus_tag="pgaptmp_001930"
                     /pseudo
     CDS             28389..29423
                     /gene="rhaT"
                     /locus_tag="ID725_RS09650"
                     /old_locus_tag="pgaptmp_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(29420..30268)
                     /gene="rhaR"
                     /locus_tag="ID725_RS09655"
                     /old_locus_tag="pgaptmp_001931"
     CDS             complement(29420..30268)
                     /gene="rhaR"
                     /locus_tag="ID725_RS09655"
                     /old_locus_tag="pgaptmp_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGV7850542.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(30342..31178)
                     /gene="rhaS"
                     /locus_tag="ID725_RS09660"
                     /old_locus_tag="pgaptmp_001932"
     CDS             complement(30342..31178)
                     /gene="rhaS"
                     /locus_tag="ID725_RS09660"
                     /old_locus_tag="pgaptmp_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGV7850543.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            31466..32935
                     /gene="rhaB"
                     /locus_tag="ID725_RS09665"
                     /old_locus_tag="pgaptmp_001933"
     CDS             31466..32935
                     /gene="rhaB"
                     /locus_tag="ID725_RS09665"
                     /old_locus_tag="pgaptmp_001933"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGV7850544.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            32932..34191
                     /gene="rhaA"
                     /locus_tag="ID725_RS09670"
                     /old_locus_tag="pgaptmp_001934"
     CDS             32932..34191
                     /gene="rhaA"
                     /locus_tag="ID725_RS09670"
                     /old_locus_tag="pgaptmp_001934"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGV7850545.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            34366..35190
                     /gene="rhaD"
                     /locus_tag="ID725_RS09675"
                     /old_locus_tag="pgaptmp_001935"
     CDS             34366..35190
                     /gene="rhaD"
                     /locus_tag="ID725_RS09675"
                     /old_locus_tag="pgaptmp_001935"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGV7850546.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            35200..35514
                     /gene="rhaM"
                     /locus_tag="ID725_RS09680"
                     /old_locus_tag="pgaptmp_001936"
     CDS             35200..35514
                     /gene="rhaM"
                     /locus_tag="ID725_RS09680"
                     /old_locus_tag="pgaptmp_001936"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGV7850547.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(35555..36949)
                     /locus_tag="ID725_RS09685"
                     /old_locus_tag="pgaptmp_001937"
     CDS             complement(35555..36949)
                     /locus_tag="ID725_RS09685"
                     /old_locus_tag="pgaptmp_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGV7850548.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            37427..37873
                     /gene="frvA"
                     /locus_tag="ID725_RS09690"
                     /old_locus_tag="pgaptmp_001938"
     CDS             37427..37873
                     /gene="frvA"
                     /locus_tag="ID725_RS09690"
                     /old_locus_tag="pgaptmp_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGV7850549.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            37884..39323
                     /gene="frvB"
                     /locus_tag="ID725_RS09695"
                     /old_locus_tag="pgaptmp_001939"
     CDS             37884..39323
                     /gene="frvB"
                     /locus_tag="ID725_RS09695"
                     /old_locus_tag="pgaptmp_001939"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGV7850550.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            39313..40383
                     /gene="frvX"
                     /locus_tag="ID725_RS09700"
                     /old_locus_tag="pgaptmp_001940"
     CDS             39313..40383
                     /gene="frvX"
                     /locus_tag="ID725_RS09700"
                     /old_locus_tag="pgaptmp_001940"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGV7850551.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            40383..42131
                     /gene="frvR"
                     /locus_tag="ID725_RS09705"
                     /old_locus_tag="pgaptmp_001941"
     CDS             40383..42131
                     /gene="frvR"
                     /locus_tag="ID725_RS09705"
                     /old_locus_tag="pgaptmp_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGV7850552.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(42181..>43220)
                     /gene="yiiG"
                     /locus_tag="ID725_RS09710"
                     /old_locus_tag="pgaptmp_001942"
     CDS             complement(42181..>43220)
                     /gene="yiiG"
                     /locus_tag="ID725_RS09710"
                     /old_locus_tag="pgaptmp_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGV7850553.1"
                     /translation="SSAIIVAIMALGLTGCDDKKAETETPLPANSQPAAPAPEAKPTE
                     APVAKADAKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLKDFKQ
                     GPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTI
                     NEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELK
                     NIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKG
                     GMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREY
                     NEMVDDYNSLR"
BASE COUNT        10649 a        11058 c        10973 g        10540 t
ORIGIN      
        1 aaaatccttt tgaaataaat aagaaccaga agcctagcgc cagtcaacaa caggaaatga
       61 taatgagtta acaccccgga atttttcaga atttataaaa ggcaaattac aggcacaaaa
      121 aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat ttgctgcccg ggatgttgaa
      181 acgcttgttg aagcggtcaa cacggccacc ggtagcaaca tcacgctgtt tgccagtgaa
      241 gaacgggtgg cacttgctgc acacgtcgag gttcaggtca tgaccaacgg tggagcggat
      301 tttcattacg ttaccgcaag agcagctagc agtaatttct tcgtatttcg ggtgaatatc
      361 ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc ttccagccct aacgccagac
      421 accacgcgat gttaaaagta tagcttcaat acgatcattt cgtacgaagg cgcgaaatca
      481 tacagaaatt aaccagcata tgcaaactga tccgcactct tctacggcaa tgtgtatact
      541 aacccaccga atttcaagtc aggatgatgc tatgcccgtt gcccacgttg ccttgcccgt
      601 tccgcttcct cgtacctttg actatctcct gccagaaggc atggcggtta aagccgggtg
      661 tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc ggggttgtgg tgtcagttag
      721 cgacgtcagt gagctgccgc ttaacgagct aaaagcggta gtcgaagtgc tggatagtga
      781 gccggtgttt actcactccg tctggcgatt gctgctctgg gcggcggatt actatcatca
      841 tccgattggc gatgtgctgt ttcatgcctt gccgatttta ctgcgccagg ggcggcctgc
      901 ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa ggccatgcgg tggatctgaa
      961 cagcctgaaa cgttcgccaa agcaacaaca ggcactggcg gcattgcggc aaggcaaaat
     1021 ctggcgcgac caggttgcca cgcttgaatt taatgatgcc gcgttacagg cgctgcgcaa
     1081 aaaaggtctg tgtgatttag caagtgaaac accagagttt agcgactggc gaacgaacta
     1141 tgccgtttct ggtgaacggt tgcgattgaa taccgaacag gccaccgccg ttggcgcaat
     1201 tcatagcgcg gcggatacct tctctgcttg gctgctggcg ggcgttacag gttccggtaa
     1261 aacagaggtt tatttgagcg tactggaaaa cgtgctcgct cagggcaaac aggcgctggt
     1321 gatggtgccg gaaatcggcc tgacaccgca aactatcgcc cgttttcgtg aacgttttaa
     1381 tgcccccgta gaagttctgc attccggcct gaacgacagc gagcgtcttt cggcgtggtt
     1441 gaaagcgaaa aatggtgagg cggcgattgt catcggcacc cgctccgcgc tgtttacgcc
     1501 gtttaaaaat ctcggtgtga ttgtcattga tgaagagcac gacagttcct acaagcagca
     1561 ggaaggctgg cgctatcatg cccgcgacct ggcggtttat cgtgcgcaca gcgagcaaat
     1621 cccgattatt cttggctccg caacacccgc actggaaacg ttatgcaacg tccagcagaa
     1681 aaaataccgc ctgctgcgcc tgacgcgtcg ggcggggaat gcgcgtccgg caattcaaca
     1741 tgtgctggat ttaaaaggtc agaaggtgca ggcggggctg gctccggcgt taatcacccg
     1801 tatgcgccag catttacagg ccgacaacca ggttatcctc tttcttaacc gccgtggctt
     1861 tgcgcctgcc ttgctgtgcc acgactgcgg ctggattgcc gaatgtccac gttgcgatca
     1921 ctactacacg ctgcatcagg cgcaacagca tttgcgctgt caccactgtg acagccaacg
     1981 cccggtgccg cgccagtgcc cttcctgcgg ttccacgcac ctggtacccg tggggctggg
     2041 caccgaacag cttgaacaga cgctcgcgcc gttattccct ggtgtgccca tttctcgtat
     2101 cgaccgcgat accaccagcc gcaaaggagc gctggaacaa caactggcgg aagtgcatcg
     2161 cggcggcgcg aggatattga ttggcacgca aatgctggcg aaaggtcacc actttccgga
     2221 tgtgacgctg gtagcgttac tggatgtgga cggcgcgctg ttttctgccg attttcgctc
     2281 ggcagagcgt tttgcccagc tatacaccca ggtagcaggc cgtgccgggc gtgcgggtaa
     2341 acagggcgaa gtggtgctgc aaacgcatca tccggaacac ccgctattgc aaacgttgct
     2401 ctataaaggc tacgacgctt ttgccgaaca ggcactggct gagcggcgaa tgatgcaact
     2461 gccgccgtgg accagccatg tgattgtgcg tgcggaagat cataacaatc agcacgcgcc
     2521 attgttcctg caacaactgc gtaatctgat cctctccagc ccgctggcag acgacaaatt
     2581 gtgggttctc ggtccggttc cggcactggc acctaaacgt ggcggtcgct ggcgctggca
     2641 gatattgttg cagcaccctt cccgcgtgcg cttgcaacac atcattaacg gtacgctggc
     2701 actcatcaat acaataccgg attcccgtaa ggtgaaatgg gtgctggatg ttgatccgat
     2761 tgagggttaa accgctcacg atgcgaggcg gatcgaaaaa ttcaatattc atcacacttt
     2821 tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta aaaatgttgc cgatgtgaag
     2881 taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag tgagtgtgaa agcgaagaag
     2941 caggaaactg ccgcgaccat gaaagacgtt gccctcaagg caaaagtctc tacagcgacc
     3001 gtctcccgag cattaatgaa tcccgataaa gtctcccaag ccacccgtaa tcgggttgaa
     3061 aaagcggccc gtgaagtggg ttatttaccg cagcctatgg ggcgcaacgt caagcgtaat
     3121 gaatcccgca ccattctggt gattgtcccg gatatctgcg atcccttctt tagcgaaatt
     3181 attcgcggta tcgaagttac ggcggcaaat cacggatatc tggtgctgat tggcgactgt
     3241 gcgcatcaaa atcagcagga aaaaaccttt atcgatttga tcatcaccaa gcaaattgat
     3301 ggcatgttgc tgctgggttc aaggctgccg tttgatgcca gcattgagga acagcgtaat
     3361 ctgccgccga tggtgatggc gaacgaattt gcaccggaac tggagctgcc taccgttcat
     3421 atcgacaatc tgaccgccgc atttgatgca gtaaattatt tatatgagca agggcataaa
     3481 cggattggct gtatagccgg tcccgaagag atgccgctgt gtcactaccg cctgcaaggt
     3541 tacgttcagg cgctgcgtcg ctgcggcatt atggttgatc cgcaatacat cgcccgtggc
     3601 gacttcacct tcgaagccgg aagcaaagcg atgcagcagc tgcttgatct tccacaaccg
     3661 cctactgctg tcttctgcca tagcgatgtg atggcgctcg gcgcactttc tcaggcaaaa
     3721 cgccaggggc tgaaagtccc ggaagacctt tccataatcg gttttgataa catcgacctg
     3781 acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc cgcgttacga aatcggtcgg
     3841 gaagctatgc tgttattgct tgatcaaatg caggggcaac acgttggcag tggctctcgt
     3901 ttaatggact gcgaacttat catccgggga tcaacacgcg cgttacctta aagtaaacct
     3961 ttaagactgc cgtgactggt caaaggcccg ccgcttaagt aacatggcgg gctgacgaac
     4021 gaataaatac agcgaaacga tagtggcaca acgagattat gtacgccgca gccaaccggc
     4081 accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa cgaaatctgc ctgcggtttc
     4141 tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc tttatcggtg gtctgtactt
     4201 cattacgcat cacaagaaag aagagtccga gacgctgcaa agccagaaag tgaccggaaa
     4261 cggactacca ccaaaaccag aagaacgctg gcgctacatc aaagagctgg aaagtcgcca
     4321 gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt gaagtgaaaa cgccggagca
     4381 actgacacca gaacaacgtc agcttcttga acaaatgcag gctgatatgc gccagcagcc
     4441 aacgcagctg gttgaagttc cgtggaatga acagacgccg gaacagcgtc agcaaacgct
     4501 acagcgccaa cgtcaggcgc agcaattagc ggaacagcaa cgcctggctc agcagtcgcg
     4561 tacgactgaa caaagctggc agcagcagac gcgtacgtcg caagccgcgc cggtacaggc
     4621 gcaaccgcgc cagtctaaac cggcttctac tcagcagccg taccaggatc tgctgcaaac
     4681 ccctgcgcac acgaccgcgc agtcgaagcc gcagcaagct gcgccggtta ctcgtgccgc
     4741 tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc cgctggatgg tgcagtgcgg
     4801 ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct cagctggcgt tcgaaggctt
     4861 tgactcaaaa attaccacca acaatggctg gaatcgtgtg gtcattggcc cggtgaaagg
     4921 caaagagaac gcagacagca ccctcaatcg gttgaagatg gcgggtcata caaactgcat
     4981 tcggctcgcc gccgggggtt gaaaccctca aaatcccccc catctataat tgcattatgc
     5041 cccgtacttt tgtacggggt ttgtactctg tattcgtaac caaggggtca gctcgtgaca
     5101 actatagtaa gcgtacgccg taacggccat gtggtcatcg ctggtgatgg tcaggccacg
     5161 ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc gccgtctgta caacgacaaa
     5221 gtcatcgcgg gctttgcggg cggtactgcg gatgctttta cgctgttcga actgtttgaa
     5281 cgtaaactgg aaatgcatca gggccatctg gtcaaagccg ccgttgagct ggcaaaagac
     5341 tggcgtaccg atcgcatgtt gcgcaaactt gaagcactgc tggcagtcgc ggatgaaact
     5401 gcatcgctta tcatcaccgg taacggtgac gtggtgcagc cagaaaacga tcttattgct
     5461 atcggctccg gcggccctta cgcccaggct gcggcgcgtg cactgttaga aaatactgaa
     5521 cttagtgctc gtgaaattgc tgaaaaggcg ttggatattg caggcgacat ttgcatctat
     5581 accaaccatt tccacaccat cgaagaatta agctacaaag cgtaaggatc tcccatgtct
     5641 gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc acatcatcgg ccaggacaac
     5701 gccaagcgtt ccgtggcgat tgctctgcgt aaccgctggc gtcgcatgca gctcaacgaa
     5761 gagctgcgcc atgaagtgac cccgaaaaat atcctgatga tcggcccgac cggtgtcggt
     5821 aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg cgccgttcat caaagttgaa
     5881 gcgaccaaat tcaccgaagt gggctacgtc ggtaaggaag tggattctat tattcgcgat
     5941 ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta tcgagaaaaa ccgttatcgc
     6001 gctgaagaac tggcagaaga acgtattctc gacgtgctga tcccacctgc taaaaacaac
     6061 tggggacaga ccgaacagca gcaggaaccg tccgctgctc gtcaggcatt ccgcaaaaaa
     6121 ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg atcttgccgc agcaccgatg
     6181 ggcgttgaaa ttatggctcc tccaggcatg gaagagatga ccagccagct gcagtccatg
     6241 ttccagaacc tgggcggtca gaagcaaaaa gcgcgtaagc tgaaaatcaa agacgccatg
     6301 aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc cggaagagct gaagcaagac
     6361 gctatcgacg ctgttgagca gcacgggatc gtgtttatcg acgaaatcga caaaatctgt
     6421 aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag gcgttcagcg agacctgctg
     6481 ccgctggtag aaggttgcac cgtttccacc aaacacggga tggtcaaaac tgaccacatt
     6541 ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt ctgacctgat cccggaactg
     6601 caaggtcgac tgccaatccg cgttgaactg caggcactga ccaccagcga cttcgagcgt
     6661 attctgaccg agccgaatgc ctctatcacc gtgcagtaca aagcactgat ggcgactgaa
     6721 ggcgtaaata tcgagtttac cgactccggt attaaacgca tcgcggaagc ggcatggcag
     6781 gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac acactgttct ggagcgttta
     6841 atggaagaga tttcctacga cgccagcgat ttaagcggtc aaaatatcac tattgacgca
     6901 gattatgtga gcaaacatct ggatgcgttg gtggcagatg aagatctgag ccgttttatc
     6961 ctataatcgc gttcaatcat tttcatcatt gtttgatggg gctgaaaggc cccattttta
     7021 ttggcgcgta ttatgactga acaacaaatt agccgaactc aggcgtggct ggaaagttta
     7081 cgacctaaaa ccctccccct cgcctttgct gcaattatcg tcgggacggc gctggcatgg
     7141 tggcaaggtc attttgatcc gctggtcgcc ctgctggcac tgattaccgc cgggctatta
     7201 cagatccttt ctaacctcgc caatgattac ggcgatgcgg taaaaggcag cgataaacct
     7261 gaccgtatcg gaccgctacg cggcatgcaa aaaggggtca ttacccagca agagatgaaa
     7321 cgggcgctca ttattaccgt cgtgctcatc tgtctctccg ggctggcact ggttgcagtg
     7381 gcgtgccata cgctggccga ttttgtcggt ttcctgattc ttggcgggtt gtcgatcatt
     7441 gccgctatca cctacaccgt gggcaatcgt ccttatggtt atatcggtct gggtgatatt
     7501 tccgtactgg ttttctttgg ctggttaagc gtcatgggga gctggtattt acaggctcat
     7561 acattgattc cggcactgat ccttccggcg accgcatgcg gcctgctggc aacggcagta
     7621 ctgaacatta ataacctgcg tgatatcaat agcgaccgcg aaaacggcaa aaacacgctg
     7681 gtggtgcgct taggtgaagt gaacgcgcgt cgttatcatg cctgcctgct gatgggctct
     7741 ctggtatgtc tggcgctgtt taatctcttt tcgctgcata gcctgtgggg ctggctgttc
     7801 ctgctggcgg caccgttgct ggtgaagcaa gcccgttatg tgatgcggga aatggacccg
     7861 gtggcgatgc gaccgatgct ggaacgcact gtcaagggag cgttactgac taacctgctg
     7921 tttgttgtag ggatattcct aagccagtgg gcagcataac tgacaaatat caattaacaa
     7981 ttgatgattt tgccaacaga ccacatagcg cgatatactg aaaattctcg cagcaactga
     8041 atgttaagcc tatgaaatac gatacttccg agctttgtga catctatcaa gaagatgtta
     8101 acgtcgtgga accgctgttc tccaactttg gcggacgggc gtcgtttggc ggacaaataa
     8161 tcacggtaaa atgtttcgag gacaacgggt tgctgtacga tctgctcgaa cagaatggcc
     8221 gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg tcgcgcactg gtcgatgctg
     8281 aactggcgcg tctggcagta caaaatgaat gggaaggtct ggtcatttac ggcgcggtgc
     8341 gtcaggtaga tgacctggaa gagttggata tcggcatcca ggcgatggcg gcaattccgg
     8401 ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg cgtcaatttt ggcggtgtta
     8461 ccttcttctc cggcgaccat ctttatgccg acaataccgg gattattctt tcagaagatc
     8521 cgctggatat tgaataagta tctgccatgc gttaaaaggg gaattaaatt cccctttttt
     8581 atacatggac gtattcatgc agaatattat tcttttattt atatatttat tacttatagc
     8641 cgttaaccat ttccgctaca aatttctttt atctggcaac gcgggtgaaa tgattttata
     8701 ttttgctaac gtatttttta acccacttgc cttatcattt attatcgcca caatcatctg
     8761 tataacgata aaaaaaagaa gcagccgcag ctttattcgc ggcacttgtt ggttaatggg
     8821 attatttctt atctatagca gctatacggt gtgggaacga cacagcatat gggattatac
     8881 tttcccggag ccaggtatca ccgtcacggt tccgagcaaa cagtgggttg ccaatatagt
     8941 aaaacaagga ccaaccctgg tgacccggga tgcccatgct attttagcta ttgttgcctt
     9001 ctcacaaaat gaactcggtg tgcattcaat cgaggaactt accgcgactc aaaacggaac
     9061 tgcagaaatg catgtttgtg atgttcaggg atttgcctgc gcttatcagg aaggagtgca
     9121 aacaatgagt gatggcaaag taagacatgc tattttcatg actctactgg ataacactaa
     9181 tttgatacaa ctggtcgcag ctatcgaccc agattatttg gacgaatatc aagaagaagt
     9241 aatgaaaatg atgctttctg ctcgtcaatg atctgatgtg catgttaata ataatggatt
     9301 gatatgagat acgttatctt attaatacta tggataataa ctgtttttct tagtcatctt
     9361 cagcacagta acttgcttac tggagatcca gggatggttg gcgagtatat cggttcagta
     9421 cttcttggac cgttattact tcccgtgctt gtgtcaggta ttctgtgcac attcactaaa
     9481 aaaacgcgta acttcgccag ttttactcgc gggtgctgtt gggtattggg agtcctgctt
     9541 ttatccaacg tcggcaatac gtttcgtctg ttcaccccgt ggcactatac gtttgagaag
     9601 gcagctattt ccgtgactgt cccaaaccga cactggaata cggtttctat tagtactgat
     9661 aaaaccattg atataagaag tgaagataat agcgtcttta tttctgcttt ccgcctgccc
     9721 gcaggacgta gcgccgatga ctcgctggaa gagttaaaga agatgcagcg agacaatctc
     9781 aaggatcaat acaatgaaga aaccttccag tttcatgatt gtaatgccaa acatttcacc
     9841 tgtaagtatc aggatgtgtt gataaatttt gatggtcagc agaaaagaac catcagtgtt
     9901 tatcttgaag acacccccag ggctgtgggg atcattgcat taatggagcc agatacggca
     9961 gataaatatc gccaacaggc gatggagatt atgttgtctg ctaaaaatac agtgaagtaa
    10021 aaaataaggc gccttgcggc gcctctgcgt ggaagagatt aaacctcttc catgcgaccc
    10081 agcagggcct gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca
    10141 cgctccagct cttcgcgctg atgctgggca ttttgaactt cctgcgacaa tgagttgttt
    10201 ttttctttca gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt
    10261 acttttgctt ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc
    10321 aaggcgttga tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga
    10381 gcgcacttta gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct
    10441 tgcagattac ggtttgccac acttttcatc cttctcctgg tgacataatc cacaccaatc
    10501 gaaaatgtta ataaatttgt tgcgcgaatg ttctaacaaa catgcatcat gtacaatcag
    10561 atggaataaa tggcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga
    10621 tatttctcgt ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg
    10681 tccgtgactt tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac
    10741 atcaaccttg aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt
    10801 cggtgtgggt tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat
    10861 cagtgtcatt tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg
    10921 cgcgcatctt aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg
    10981 caaagttatt ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt
    11041 ttacgggctt tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg
    11101 cagcgttgaa agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa
    11161 ttttgtgcag gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct
    11221 ggcgttaacg gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg
    11281 tctactgatt gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc
    11341 agcgcgtgac ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt
    11401 taccggcggc agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc
    11461 gattgtaggt gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg
    11521 tgttgtggaa gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga
    11581 ctacgggaca attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca
    11641 ccagctcccg cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg
    11701 aatttgagca aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg
    11761 ccacccaaag ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa
    11821 ttgcagctat cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg
    11881 gcaagcctat ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc
    11941 atttaaaacg tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc
    12001 cgtacttttc tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc
    12061 gtgcgcgtcg tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga
    12121 ctcagggccg tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca
    12181 tccataccct ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc
    12241 tgccagaagt gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg
    12301 gcacgcgtat tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt
    12361 tgtgcgtgaa agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga
    12421 acactggcga gaaagcggtg aaatcagaaa acggcctact gaccaccatc gcctgcggcc
    12481 cgactggcga agtgaactat gcgttggaag gtgcggtgtt tatggcaggc gcatccattc
    12541 agtggctgcg cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca
    12601 ccaaagtgca aaacaccaat ggtgtgtatg tggttccggc atttaccggg ctgggtgcgc
    12661 cgtactggga cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta
    12721 accacattat acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag
    12781 cgatgcaggc cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag
    12841 caaacaattt cctgatgcag ttccagtctg atattctcgg cacccgcgtt gagcgcccgg
    12901 aagttcgcga agtcaccgca ttgggtgcgg cctatctcgc aggcctggcg gttggcttct
    12961 ggcagaacct cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca
    13021 tcgaaaccac tgagcgtaat taccgttacg caggctggaa aaaagcggtc aaacgcgcga
    13081 tggcgtggga agaacacgac gagtaatgtc aggcacctga atcgattgcc tgatgctgat
    13141 cgcctgatgc gacgcttacg cgtcttatca ggcctacgag ccaatgccat tttgtgtagg
    13201 cgggataagg cgttcacgcc gcatccggca tcacgcagac tctgaaaccg tgcgggggca
    13261 accccgcaca catcaataat cccacccttc ccctgtgcta cacttcgcgc cattccttac
    13321 tgcttagagt ttgctatgag acgagaactt gccatcgaat tttcccgcgt caccgaatct
    13381 gcggcgctgg ctggctacaa atggttagga cgcggcgata aaaacaccgc ggacggcgcg
    13441 gcggtaaacg ccatgcgtat tatgctcaac caggtcaaca ttgacggcac catcgtcatt
    13501 ggtgaaggtg aaatcgacga agcaccgatg ctctacattg gtgaaaaagt cggtactggt
    13561 cgcggcgacg cggtagatat tgctgttgat ccgattgaag gcacgcgcat gacggcgatg
    13621 ggtcaggcta actcgctggc ggtgctggca gtaggcgata aaggctgttt cctcaacgcg
    13681 ccagatatgt atatggagaa gctgatcgtc ggaccgggag ccaaaggcac cattgatctg
    13741 aacctgccgc tggcggataa cctgcgcaat gtcgcggcgg cgctcggcaa accgttgagc
    13801 gaactgacgg taacgattct ggctaaacca cgccacgatg ccgttatcgc tgaaatgcag
    13861 caactcggcg tacgcgtatt tgctattccg gatggcgacg ttgcggcctc aattctcacc
    13921 tgtatgccag acagcgaagt tgacgtgctg tacggaattg gtggcgcgcc ggaaggcgtg
    13981 gtttctgcgg cggtgatccg cgcattagat ggcgacatga acggtcgtct gctggcgcgt
    14041 catgacgtca aaggcgacaa cgaagagaat cgtcgcattg gcgagcagga gctggcacgc
    14101 tgcaaagcga tgggcatcga atccggtaaa gtattgcgtc tgggcgatat ggcgcgcagc
    14161 gataacgtca tcttctctgc caccggtatt accaaaggcg atctgctgga aggcattagc
    14221 cgcaaaggca atatcgcgac taccgaaacg ctgctgatcc gcggcaaatc acgcaccatt
    14281 cgccgcattc agtctatcca ctatctggat cgcaaagacc cggaaatgca ggtgcacatc
    14341 ctctgattga tttgatcgat tgagccttcc agtccttcgg gactggaatt tttttgttcg
    14401 gagaacgaag ataaggcaat tcaatcaaaa caggagaaaa acatggctga ttgggtaaca
    14461 ggcaaagtca ctaaagtgca gaactggacc gacgccctgt ttagtctcac cgttcacgcc
    14521 cctgtgcatc cgtttaccgc cgggcaattt accaagcttg gccttgaaat cgacggcgaa
    14581 cgcgtccaac gcgcctactc ctatgttaac tcgcccgata atcccgatct ggagttttac
    14641 ctggtcaccg tccccgatgg caaattaagc ccacgattgg cggcactgaa accgggcgat
    14701 gaagtgcagg tggttagcga agcagctggc ttctttgttc tggatgaagt accagattgc
    14761 gaaacgctat ggatgctggc aaccggtaca gcgattggcc cttatttatc gattctgcaa
    14821 ctaggtaaag atttagatcg cttcaaaaat ctggtcctgg ttcacgccgc acgttatgcc
    14881 gccgacttaa gctatttgcc actgatgcaa gaactggaaa aacgctacga aggcaaactg
    14941 cgcattcaga cggtggtcag tcgggaaacg gcagcggggt cgctcaccgg acggataccg
    15001 gcattaattg aaagtggtga actggaaagc gcgattggcc tgccgatgaa taaagaaacc
    15061 agccatgtga tgctgtgcgg caatccacag atggtgcgcg atacgcaaca gttgctgaaa
    15121 gagacccggc agatgacgaa acatttacgt cgccgaccgg gtcacatgac agcggagcat
    15181 tactggtaag cggttactta tcgataaacg gcacgatgag caaatccgca ctcaacttat
    15241 tgatcatccc gcgatatgcc ggcatcaaac ggttgataaa tgagtggtga tgaccacaga
    15301 caaggaggtc gcactgctct ttttgcataa tttccagcag tgtttccggc atttctccgc
    15361 gttcaatacg cagttttgtc ttcggccatt gaatattttt cgtcagttta tacagcttgt
    15421 tatccgactt attcttcaac aattgaagaa tatcttctgt tgcagggaag tagatacccg
    15481 gatacaactc gcttaagcca tcatcaatat gaattaacgt caggtgagcg tcattatgtc
    15541 tggcgagctc caaggcttta ttcaccagta aggcatcttc ttcattcccg gaaattgcca
    15601 cgccaatgtg tttataagcc atgtttaact ccttctaaag caactccatc aagctaacga
    15661 acgcagtgat aactcaaaaa taatcatctc ggcctggcat gagaaggtga aggccgcatc
    15721 aagctcaact tgcccctcca gatccttaat cttataggcc acgctggccc cttcatctgc
    15781 agaggatgca gcggcggcaa gctctttcag tttggtcata aaggactctg cttcttcgcg
    15841 ggtggcaaaa aagcgcgaaa acttactggt acagttgtcg ttatcaatca ccgtaccgat
    15901 atcaatcgca catcctttag tactgcattt atctacgaca tctttcatag ggcacctctg
    15961 tatattcgcc tctctgtcga ttacgactta agaataatgc gccgttaagg gaagaacaaa
    16021 caagtaatga tcttatggat taccattttt tttgatattg cgtaaggtga agagttcaga
    16081 aggattttcc gggtattgtc atttgcatcg tggaaataac aatcccggaa aaaaatagca
    16141 gtaagagata tattaaaggt taattacttg atttattgtc ggctttatac ttcacatcct
    16201 gagtatcttt accatattta ttttcgcctt gtgtgccaac aaacgcacca agatcgataa
    16261 gcatcatcac cagaatcaac gtcgggacaa aacgccctac cgcccattgc cagacacccg
    16321 gtaaaatcgc ccagttaccc gccagtagca tccacgccac aatcatcaga aatgcccatg
    16381 cgccggaacg cccgcgatca tgcaagcgct taacggttac tgccgctgtt ggccagagca
    16441 agcacacaag gcaaaacgcc gcggtctgaa tatcgagtaa atttttaccc gccagtgaga
    16501 aaagcaccag catgcctgcg aaccacaggc ctatccaaat ccagaaatca cggcgtccaa
    16561 tacgcccttt aaatgagaat aaccattgct gtatggtcat gtaagttcct tgatggttgt
    16621 cttttccagg attctaccct tttgacaagg gcgacagata tcgttttaat cggagccagt
    16681 cataacaaaa ggtactgtca atgaagccag ggtgtacgct gttttttctc ttatgttctg
    16741 cattaaccgt tacaacaacg gcgcatgcac aaacaccaga tacggcaacg accgcgcctt
    16801 atctgctggc tggagcccct actttcgatc tctccatcag ccagtttcga gaagacttta
    16861 acagccagaa tcccagcctg ccactgaacg aatttcgtgc catcgacagc agtcccgaca
    16921 aagccaatct cactcgtgct gccagtaaaa ttaacgagaa cttgtatgct tctacagcgc
    16981 tggagcgcgg taccttaaaa atcaaaagca ttcaaatgac atggctaccc atccaggggc
    17041 cagagcaaaa agccgcgaaa gcgaaagctc aggaatacat ggcagcggtg atccgcacac
    17101 tcaccccatt aatgaccaaa acacaaagcc agaaaaaact gcagtcgcta ctaacggcgg
    17161 ggaaaaacaa acgttattac accgagacag aaggtgcact gcgctatgtt gtcgcggaca
    17221 acggcgaaaa ggggctgacc ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc
    17281 ttgaaggttt gaataaatga caaaaagcaa agcctttgtg ccgatgaatc tctatactgt
    17341 ttcacagacc tgctgccctg cggggcggcc atcttccttt attcgcttat aagcgtggag
    17401 aattaaaatg cgacatcctt tagtgatggg taactggaaa ctgaacggca gccgccacat
    17461 ggttcacgag ctggtttcta acctgcgtaa agagctggca ggtgttgctg gctgtgcggt
    17521 tgcaatcgca ccaccggaaa tgtacatcga tatggcgaag cgtgaagctg aaggcagcca
    17581 catcatgctg ggtgcgcaaa acgtggacct gaacctgtcc ggcgcattca ccggtgaaac
    17641 ctctgctgct atgctgaaag acatcggcgc acagtacatc atcatcggtc actctgaacg
    17701 tcgtacttac cacaaagaat ctgacgaact gatcgcgaaa aaattcgcgg tgctgaaaga
    17761 gcagggcctg actccggttc tgtgcatcgg tgaaaccgaa gctgaaaacg aagcgggcaa
    17821 aactgaagaa gtttgcgcac gtcagatcga cgcggtactg aaaactcagg gtgctgcggc
    17881 attcgaaggt gcggttatcg cttacgaacc tgtatgggca atcggtactg gcaaatctgc
    17941 aactccggct caggcacagg ctgttcacaa attcattcgt gaccacatcg ctaaagttga
    18001 cgctaacatc gctgaacaag tgatcattca gtacggcggc tctgtaaacg cgtctaacgc
    18061 tgcagaactg tttgctcagc cagacatcga cggcgcgctg gttggcggtg cgtctctgaa
    18121 agctgacgct ttcgcagtaa tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg
    18181 acaggtgccg gatttcatat ccggcactta ctttccttaa ctcttcgcct taacgcaaaa
    18241 tctcacactg atgatcctga atttcctcgg ctgaagcacg gttaagcgtc agtagatttc
    18301 gttgtgtcgc cagcaataca aatgagttat cactctgccg taccatcgcc agcccgtagc
    18361 ttcccatatg ttcccgcgcc tcaggtactt cttctgccag catcataaat gggctgcgtt
    18421 gtaccagttc gctttccgtt acccgacgcg ccaggtattc atgcccgcgc aaaccacctg
    18481 gcagtggcaa ccagcggctg ctgatgttcg ccagattgtt atccagctgt tcgcgcacat
    18541 caggacgaat acaagagata tgaatatgaa aatggttttg cgtacgcccg gtgcgggagt
    18601 tgatcgccaa agaaaccgcg cgatcgggaa ccggctggcc gtattttttg ctcataaaat
    18661 cacgcgcctg ccaggccaac caaaagaagt tcggcgttga aggatcggtc aacaaaggac
    18721 tttcagtacc gttaatacga tacgttggca tcaacagata ttgcagtggg ccattaagat
    18781 cttttaaaac cacgtatccg gcattgggtt tgacttccgc acatggcgaa ggattttgat
    18841 tttgctgctg attgggcaaa cattcctcaa ggacaatctt acgtaatgta tccgactctt
    18901 caccggttaa tttccagtaa ccaataccgg cagccacaac ggcgataact atcatcacca
    18961 aaaaaagaag acccgctttt ttcatctttt tttccctgta cctcaaagag atgcaagggt
    19021 aacgcaaaat cgtgacaaat aaaaaacccg gtcgcgagaa accaccgggt ttgataatta
    19081 tcctggggaa atcagcgttt gctgatctga tcgaacgtac cgccgttagc aaaatgctct
    19141 ttttgcgctt tcgtccagcc gccgaactct tcatcaatgg tgaataactt cagctttgga
    19201 aacgcatttt cgtacttttt cgccacctca gcgtcgcgcg gacggtagta gtttttcgcg
    19261 gcaatttcct gaccttctgg cgagtagaga tatttcaggt aggcttccgc cacctcttta
    19321 gtaccttttt tctcgaccac tttatcgacc accgacacgg ttggctctgc gaggatagac
    19381 tcactcggcg tgacgatttc gaatttatct ttccccagtt cattcgctgc cagcagagct
    19441 tcgttttccc aggaaatcag tacatcgcca attccgcgct cgacaaaagt gttagtggag
    19501 ccgcgcgcgc cagaatccag aacttcgacg tttttataca gtgcccgaac aaaatcctgt
    19561 gcttttgcct gatcgttgtt gttgtgatgc agcgcgtagc cccaggctgc caggtagttc
    19621 cagcgcgcgc caccagagct tttcggatta ggcgtgatca ccgaaacacc cagtttaatc
    19681 agatcgttcc agtcatggat ctgcttcgga tttcccttac gtaccaggaa aacaatggtg
    19741 gaagtgtacg gtgcggagtt atccggcaga cgtttgatcc actctttatc aatccgcccg
    19801 cgttccgcaa tcgcgtccac gtcataggcc agagccagcg tgacaacatc agcttcaata
    19861 ccgttgatta ccgacgttgc ttgtttgcct gaaccaccgt gtgactgacg aatcaccacg
    19921 ttatcgccag tttgctgttt ccagtgggcg ctgaatgcct tgttgtactg ttcgtacaat
    19981 tcgcgcgttg gatcatatga aacgttaaga agctgaatat cctttgccat aacgctggtt
    20041 gccgccagca aaaatgttaa ccctacgccc cacttgttca tcgcccgact ctcttatgtt
    20101 gtgttgtgat gagcaaagcg tgccagaagg ttaaccaaac attaaagaat aaaaaaagat
    20161 tggctataac ttgcgggtat atgttggggg ttaaaaaggc ggaagaaatc cgcctcatat
    20221 tgctgacaaa gtgcgcttta tcgatgccgg atgcggcgcg aacgccttat ccggcctaca
    20281 aaagtttgta aattcaataa attgtagaat tcacgtaggc ctgataagcg aagcgcatca
    20341 ggcatttttg cttctgtcat cggtttcagg ctaaaggaat ctgccttttt ccgaaatcat
    20401 tagtacagtt ttttcgcgca gtccagccag tcgcctttga acggacgctt catgttttcg
    20461 atagcgtcga tgatgtcgtg gtgaaccagc tgttcgttct gtatacctac gcaacgaccg
    20521 ccgtaacctg ccagcagcag atcgatagcg taagcgccca tacgggaagc cagaatacgg
    20581 tcgtaaggca ccggagaacc accgcgctgg atgtggccca gcacagttgc gcgggtttca
    20641 cgaccggttt ctttctcgat gaaatgcgcc agttcgtcaa catcacacat atgttcggta
    20701 atcgccacga tcgcgtgttt tttacctttc gcgatacccg ctttgatttc gtttaccagg
    20761 tcttcacggc tgaattcaac ttccggaacc acaacgaatt cacagccacc ggcaatggcc
    20821 gcagccagcg tcagatcgcc acaataacgg cccatcactt ccaccacgga aatacgctgg
    20881 tgagaagaag aggtgtcacg cagacggtcg atcgcttcta caacggtgct cagcgcagtg
    20941 aagaaaccga tagtgtagtc agtgcctttg atgtcgttgt cgatagtgcc cggcaggccg
    21001 atgcacggga agcccatttc ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg
    21061 ataaccacca gcgcatcgat cccacgtttt ttcaggtttt cgatagccac ggcgcggatg
    21121 ttctcgtcgc ggaattccgg gaaacgcgca gaaccgagga acgtaccgcc acggttgatc
    21181 atgtcagaaa cgctgtaacg gtctagctgt accatacggt cttcatacag acccagatag
    21241 ccgtcataaa tgcccattac ttccagacct tctgtcagcg cagaacgaac aaccccgcga
    21301 attgcggcgt tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat tttcttaatc
    21361 atgactacct ctgaactttg gaatgcaaaa taaaatctgt tgccggaagt cttcttgcac
    21421 atcgaagtga tccaacgaat gtgcaaatag tataacaatc acttcctgct gaattgattc
    21481 aggtcaggcc aaatggcggt attttataca caaaatgcgg gtctggctct cttttatact
    21541 gattatgaaa gcatagaccg tttaccctcc ctaggtacga cggaacaggg gtcctgatgg
    21601 ataatcacat ccgatcccgg aaaacgccgt aaaatagcct gctctacctg atccgccacc
    21661 atatgtgcct gaaccaaagg cagagagtct tccatttcca aatgaatctg aataaagcgg
    21721 gtcggccctg actgccgcgt gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc
    21781 acgatatcaa taatttcttg ccgttcctca tcaggtaatg cgcgatccag taatgactgt
    21841 accgcctcat atcccatgcg taacgcgcta tataaaatat agatgccgat tcccaatgca
    21901 aacagagcat cggcgcgatg ccagccgtac caggacaacc ccagcgccag cagaattgcg
    21961 ccgttcatca taacatcaga ctggtaatgt agcatatcag cccgcaccgc ctggctttgc
    22021 gtccggcgca ccacccaacg ctgaaacgag acaaggataa tcgtacaaat tagcgccaca
    22081 attgtcacga taaccccgac gcctggatct gtcatcggtg ttggagatat cagatgttga
    22141 atgcctgtca aaaacaggaa tagtgccgaa ccggagataa acatactttg cgccagcgcc
    22201 gcgagggact cagctttacc gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa
    22261 tatcgcacca ccaataaatt cgtcaacgac gcgccgatat ccaccagcga atccaccagc
    22321 gcggcgagaa tactcaccga cccggtatac caccatgcaa aaattttaat cagcaatagc
    22381 agtgaagcca tcgccgtcgc agcaatcgcc gcccgactga ccagccgtcc ataagattga
    22441 ttcataaata ctcccgctat caactgacgc tagtataacg gaagcaaatc atctgcaatg
    22501 cattaagcag caggcaaatt gaggataaaa aaaaccccca catcatgtgg gggaagacag
    22561 ggatggtgtc tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact
    22621 caatagcttc aacgatgaac ttttttgcca ttgcgtcacg tcacgcaact gctccattcg
    22681 ttgttgatgt ttctcgttta aaaccgcttg ctgctccggc gttaacaggc gatacatttg
    22741 gttgcggact tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc
    22801 ctgtgcgcgc acagcgtttt catcaaaatt ttctgcggtg acaagacgat gcattgtctc
    22861 cagttcgcta acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat
    22921 ctgctgacgc tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg
    22981 ctgcgtaagt tcttcacccg gatgccagtt atcgcctgaa ccgacttcag cagcgtggct
    23041 taatgaactg actgccagcg ttgaggccat gacggcagcg gtaactatgc gcatcatttg
    23101 ctcccaaaat ctttctgtcg cgattcaacg atagagagtt tacgattcag gctgcaaaca
    23161 tgcgtcaggg ggtgtaaaac aacgtaaagt catggattag cgacgtctga tgacgtaatt
    23221 tctgcctcgg aggtatttaa acaatgaata aaatcctgtt agttgatgat gaccgagagc
    23281 tgacttccct attaaaggag ctgctcgaga tggaaggctt caacgtgatt gttgcccacg
    23341 atggggaaca ggcgcttgat cttctggacg acagcattga tttacttttg cttgacgtaa
    23401 tgatgccgaa gaaaaatggt atcgacacat taaaagcact tcgccagaca caccagacgc
    23461 ctgtcattat gttgacggcg cgcggcagtg aacttgatcg cgttctcggc cttgagctgg
    23521 gcgcagatga ctatctcccg aaaccgttta atgatcgtga gctggtggca cgtattcgcg
    23581 cgatcctgcg ccgttcgcac tggagcgagc aacagcaaaa caacgacaac ggttcaccga
    23641 cactggaagt tgatgcctta gtactgaatc caggccgtca ggaagccagc ttcgacgggc
    23701 aaacgctgga gttaaccggt actgagttta ccctgctcta tttgctggca cagcatctgg
    23761 gtcaggtggt ttcccgtgaa catttaagcc aggaagtgct gggcaaacgc ctgacgcctt
    23821 tcgaccgcgc tatcgatatg cacatttcca acctgcgtcg taaactgccg gatcgtaaag
    23881 atggtcaccc gtggtttaaa accttgcgtg gtcgcggcta cctgatggtt tctgcttcat
    23941 gataggcagc ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg cgctggtgtt
    24001 gatgttggtt ttgatgttac ccaagctcga ttcacgccag atgaccgagc ttctggatag
    24061 cgaacagcgt cagggtctga tgattgagca gcatgttgaa gcggagctgg cgaacgatcc
    24121 gcccaacgat ttaatgtggt ggcggcgtct gttccgggcg attgataagt gggcaccgcc
    24181 aggacagcgt ttgttattgg tgaccaccga aggccgcgtg atcggcgctg aacgcagcga
    24241 aatgcagatc attcgtaact ttattggtca ggccgataac gccgatcatc cgcagaagaa
    24301 aaagtatggc cgcgtggaac tggtcggtcc gttctccgtg cgtgatggcg aagataatta
    24361 ccaactttat ctgattcgtc cggccagcag ttctcaatcc gatttcatta acttactgtt
    24421 tgaccgcccg ctattactgc tgattgtcac catgttggtc agtacgccgc tgctgttgtg
    24481 gttggcctgg agtctggcaa aaccggcgcg taagctgaaa aacgctgccg atgaagttgc
    24541 ccagggaaac ttacgccagc acccggaact ggaagcgggg ccacaggaat tccttgccgc
    24601 aggtgccagt tttaaccaga tggtcaccgc gctggagcgc atgatgacct ctcagcagcg
    24661 tctgctttct gatatctctc acgagctgcg caccccgctg acgcgtctgc aactgggtac
    24721 ggcgttactg cgccgtcgta gtggtgaaag caaggaactg gagcgtattg aaaccgaagc
    24781 gcaacgtctg gacagcatga ttaacgacct gttggtgatg tcacgtaatc agcaaaaaaa
    24841 cgcgctggtt agcgagacca tcaaagccaa tcagttgtgg agtgaagtgc tggataacgc
    24901 ggcgttcgaa gccgagcaaa tgggcaagtc gttgacagtt aacttcccgc ctgggccgtg
    24961 gccgctgtac ggcaacccga acgccctgga gagtgcgctg gaaaacattg ttcgtaatgc
    25021 cctgcgttat tcccatacga agattgaagt gggctttgcg gtagataaag acggtatcac
    25081 cattacggtg gacgacgatg gtcctggcgt tagcccggaa gatcgcgaac agattttccg
    25141 tccgttctat cggaccgatg aagcgcgcga tcgtgaatct ggcggtacag gtttgggact
    25201 ggcgattgtt gaaaccgcca ttcagcagca tcgtggctgg gtgaaagcag aagacagccc
    25261 gctgggcggt ttacggctgg tgatttggtt gccgctgtat aagcggagtt aaactccgca
    25321 tttgtaggca ggataaggcg tttacgccgc atccggcatt tgagcaggat gcctgatgcg
    25381 acgctgatag cgtcttatca ggcctacact ccgggggtta ttttccccac aaccggcggg
    25441 aaaaatcttc gatcttgccg ctcagtcggc gcttttgcat cgttcgcgtc cagcttttcg
    25501 ataacccggc agcggagagt aaacggtgat actgatcgtc atcaaacggc atatgccatg
    25561 cgatggcagc agcctcctgc acggtcacat cgctgacacg ggaaaccaac tccagcggcg
    25621 cgtccgcaga cactttccct ggcgctatca cgctatacaa ccatcccact ttacccgtgt
    25681 tttgcatcaa ctgcgctata tcgctgatat caaaatggta attgagttta tagcaaggcg
    25741 agcgcggctg gctgacctga attaatgcct ctccccagcg gaaaatatcg ccaatataaa
    25801 cattactttc cgtgagaccg tcggttgaga gattttcgcc aaacgcaggc gcaacaaaca
    25861 actccgcctg ttccggaaat tcccgcgccc agtagagata atgctcacga ggataatgac
    25921 acagcgctct gtctggtccg ccgtgaacct ttttctccgc ctgctcgtca ccttccagcc
    25981 ccagctccgt tagcatcaac tcaccatcaa cctggatttt ggcaattgcg ctgggtttgc
    26041 cttcgggata agcctgaatc ttgcctgtgt atacatcaac cggatatcgc atctgttccc
    26101 tctccacgag aaatttcccc attagaccat atttatttta tgtcaggcgt gctgttttcg
    26161 cggtaagtat ccaggcagga actcaatagc aaaccggacg aacgttagtt catccggctg
    26221 tggggattaa ggaagcaggt ttatttcacc gtacctaaaa tttcgatttt cgccgctctg
    26281 gctttgactt tacgtgacca gatagaggta agaattggca ccagaatcga agtcacaatg
    26341 actgccgtcg ctaccagcga agttgctgcc ggagccatcg gtttaaacgc aggcaccatt
    26401 tctgcaatca gcacaggtgt cgctaccgcg gcccctgcgg aactggaagc ggcaattccg
    26461 gcagtgccat cgccaccgcc aatcaattta tcggcgataa tcaacggaat accggtcacg
    26521 ataattactg ccacacccaa caggatcccc agcaaacctg tctgggcaat tacagtcaaa
    26581 tcaatggtat tgcctagtgc gaaggcaaag aatggaatca gcgtctgcac cgctttgctg
    26641 aaaaattctc gtaattcagg gtcaaggttc ccaagggcaa agcccaccag gaacggtaat
    26701 actgcgccga cgaaaacatg cggttcaaac gaggcaatcc cggcagtgcc cagaataatc
    26761 atcgtcatga gcggcccgga ctccaacgac atcaacacaa atgccccagc ttcttctttt
    26821 gtgccgtact gctgcatgat ggaagcgtaa agtccgccgt tggtcatatc catcgccgcc
    26881 accagcgcca gcgttgaaag tccggcaaag aatccaactt caacaccatg ttccggaata
    26941 atgcgcgagg caattgccgc aaccacccac gcgacggcaa ttttagttac caccagcgta
    27001 ccggatttac gcagtaccgt tcccgtcgcg cttaatttta ttgacgcccc catgcaaaaa
    27061 aaccacaccg ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa tgatccaaaa
    27121 tatttccccg cccccggcga gaaggtgtgg cacagtgcgc caaggaatag cgggacgagc
    27181 atcatccccc ccgggatttt ctcaatcaag cgttttatct gcatttccat cacctgtcgg
    27241 catattttta agggctgatg cgtaattcct caatggagca tcagtgttga tggaaacgaa
    27301 gatagacatt catttaatca ataaaagtga tcaaaaccac atcaactgaa acgctgtttt
    27361 atttttatcg gatcattgtt tttattttga aattgatcac aaaaaaacca ccgccattgg
    27421 cgatggttca ttttttaagc tgatatgcgg cgtaaacgcc tcactgcagc agacggcaaa
    27481 tgattatttt ttcgccgcga aacgtgccgc tgcttcgtcc cagttcacca cgttccagaa
    27541 ctctttgatg tagtccgggc ggcggttctg gaatttcagg tagtaagcat gctcccacac
    27601 atccaggccc agaatcggga agccggatgc gccagaaata gcttcaccca tcagcgggga
    27661 gtcctggtta gcagtagaaa ccaccgccag tttatcgcct ttcagcacca gccatgccca
    27721 gccggaacca aagcgggaag ctgccgcttt ctcaaattct gctttgaagt tatctacgga
    27781 accgaaatca cgttcgatag ccgctttcag gtcaccctgc agggtggtgc cttttttcag
    27841 acctttccag aacaggctgt ggttagcgtg accgccagcg ttgttgcgca gtacggtttt
    27901 cttgtctgct ggcagctggt ccagtttggt gatcaactct tcaaccggca ggttggcaaa
    27961 ttctggcagg ctttccaacg ccgcgttggc gttgttgacg taggtctgat ggtgtttggt
    28021 gtggtggatt tccatggtct gcttatcgaa gtgcggttcc agggcatcgt aagcatacgg
    28081 cagggatggc agggtatagc tcatattcat ctccagtatt gtcgggcggc cgattgttaa
    28141 tgccgcgtaa gcagttggtt cattatagtt aattaaatga tattgaaaat gattatcaat
    28201 gccgtacttt tcgtaagggt atggttttgc aggaaaatgc ccgagatgtg aagcaaatca
    28261 cccacttaat gccgtgattg ccagtaaatc gacaacggcg gcaacaggcg aaaggttaat
    28321 cgacagcacg atttttacac tcatctcgtc ggagatgtga cgcgacgaaa aatgatgagg
    28381 ataagaagat gaataacgcg attacgatgg ggatattttg gcatttgatc ggcgcggcca
    28441 gtgcagcctg tttttacgct ccgttcaaaa aagtaaaaaa atggtcatgg gaaaccatgt
    28501 ggtcagtcgg tgggattgtt tcgtggatta ttctgccgta ggccatcagc gccctgttac
    28561 taccgaattt ctgggcgtat tacagctcgt ttagtctctc tacgctgctg cctgtttttc
    28621 tgttcggcgc tatgtggggg atcggtaata tcaactacgg cctgaccatg cgttatctcg
    28681 gcatgtcgat gggaattggc atcgccattg gcattacgtt gattgtcggt acgctgatga
    28741 cgccaattat caacggcaat ttcgatgtgt tgattaacac cgaaggcgga cgcatgacgt
    28801 tgctcggcgt tctggtggcg ctgattggcg tagggattgt aactcgagcc gggcagttga
    28861 aagagcgcaa gatgggcatt aaagccgaag agttcaatct gaaaaaagga ctggtgctgg
    28921 cggtgatgtg cggcattttc tctgccggga tgtcctttgc gatgaacgcc gcaaaaccga
    28981 tgcatgaagc cgctgccgca ctaggcgtcg atccactgta tgtcgctctg ccaagctatg
    29041 ttgtcatcat gggcggcggc gcgatcatca acctcggttt ctgttttatt cgtctggcaa
    29101 aagtgaagga tttatcgcta aaagccgact tctcgctggc aaaaccgctg atcattcaca
    29161 atgtgttact ctcagcactg ggcgggttga tgtggtatct gcaattcttt ttctatgcct
    29221 ggggccacgc ccgcattccg gcgcagtatg actacatcag ttggatgctg catatgagtt
    29281 tctatgtatt gtgcggcggt atcgtcgggc tggtgctgaa agagtggaac aatgcaggac
    29341 gccgtccggt aacggtgttg agcctcggtt gtgtggtgat tattgttgcc gccaacatcg
    29401 ttggcatggg catggcgaat taatctttct gcgaattgag atgacgccac tggctgggcg
    29461 tcatcccggt ttcccgggta aacaccaccg aaaaatagtt actatcttca aagccacatt
    29521 cggtcgaaat atcactgatt aacaggcggc tatgctggag aagatattgc gcatgacaca
    29581 ctctgacctg ccgcagatat tgattgatgg tcattccagt ctgctggcga aattgctgac
    29641 gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt atccagcgca aagggacttt
    29701 tcaggctagc cgccaaccgg gtaatcagct tatccagcaa cgtttcgctg gatgttggcg
    29761 gcaacgaatc actggtgtaa cgatggcgat tcagcaacat caccaactgc ccgaacagca
    29821 actctgccat ttcgttagca aacggcacat gctgactact ttcatgctca agctgaccga
    29881 taacctgccg cgcctgtgcc atccccatgc tacctaagcg ccagtgtggt tgccctgcgc
    29941 tggcgctaaa tcccggaatc gccccctgcc agtcaagatt cagcttcagc cgctccgggc
    30001 aataaataat attctgcaaa accagatcgt taacggaagc gtaggagtgt ttatcgtcag
    30061 catgaatgta aaagagatcg ccacgggtaa tgcgataagg gcgatcgttg agtacatgca
    30121 gaccattacc gcgccagaca atcaccagct cacaaaaatc atgtgtatgt tcagcaaaga
    30181 catcttgcgg ataacggtca gccacagcga ctgcctgctg gtcgctggca aaaaaatcat
    30241 ctttgagaag ttttaactga tgcgccaccg tggctacctc ggccagagaa cgaaattgat
    30301 tattcgcaat atggcgtaca aatacgttga gaagattcgc gttattgcag aaagccatcc
    30361 cgtccctgac gaatatcacg cggtgaccag ttaaactctc ggcgaaaaag cgtcgaaaag
    30421 tggttactgt cgctgaatcc gcaatgatag gcgatgtcag taacgctggc ctcgctgtga
    30481 cgtagcagat gtcgggcttt catcagtcgc aggcgattca ggtatcgctg aggcgtcagt
    30541 cccgtttgct gcttaagctg ccgatgtagc gtacgcagtg aaagagaaaa ttgatccgcc
    30601 acggcatccc aattcacctc atcggcaaaa tggtcctcca gccaggccag aagcaagttg
    30661 agacgtgatg cgctgttttc caggttctcc tgcaaactgc ttttacgcag caagagcagt
    30721 aattgcataa acaagatctc gcgactggcg gtcgagggta aatcattttc cccttcctgc
    30781 tgttccatct gtgcaaccag ctgtcgcacc tgctgcaata cgctgtggtt aacgcgccag
    30841 tgagacggat actgcccatc cagctcttgt ggcagcaact gattcagtcc ggcgagaaac
    30901 tgaaatcgat ccggcgagcg atacagcaca ttagtcagac acagattatc ggtatgttca
    30961 tacagatgcc gatcatggtc gcgcacaaaa cagaccgtgc caccggtgat ggtatagggc
    31021 tgcccattaa acacatgaat acccgtgcca tgttcgacaa tcacaatttc atgaaaatca
    31081 tgatgatgtt caggaaaatc cgcctgcggg agccggggtt ctatcgccac ggacgcgtta
    31141 ccagacggaa aaaaatccac actatgtaat acggtcatac tggcctcctg atgtcgtcaa
    31201 cacggcgaaa tagtaatcac gaggtcaggt tcttacctta aattttcgac ggaaaaccac
    31261 gtaaaaaacg tcgatttttc aagatacagc gtgaattttc aggaaatgcg gtgagcatca
    31321 catcaccaca attcagcaaa ttgtgaacat catcacgttc atctttccct ggttgccaat
    31381 ggcccatttt cctgtcagta acgagaaggt cgcgaattca ggcgcttttt agactggtcg
    31441 taatgaaatt cagcaggatc acattatgac ctttcgcaat tgtgtcgccg tcgatctcgg
    31501 cgcatccagt gggcgcgtga tgctggcgcg ttacgagcgt gaatgccgca gcctgacgct
    31561 gcgcgaaatc catcgtttta acaatgggct gcatagtcag aacggctatg tcacctggga
    31621 tgtggatagc cttgaaagtg ccattcgcct tggattaaac aaggtgtgcg aggaagggat
    31681 tcgtatcgat agcattggga ttgatacctg gggcgtggac tttgtgctgc tcgaccaaca
    31741 gggtcagcgt gtgggcctgc ccgttgctta tcgcgatagc cgcaccaatg gcctaatggc
    31801 gcaggcacaa caacaactcg gcaaacgcga tatttatcaa cgtagcggca tccagtttct
    31861 gcccttcaat acgctttatc agttgcgtgc gctgacggag caacaacctg aacttattcc
    31921 acacattgct cacgctctgc tgatgccgga ttacttcagt tatcgcctga ccggcaagat
    31981 gaactgggaa tataccaacg ccacgaccac gcaactggtc aatatcaata gcgacgactg
    32041 ggacgagtcg ctactggcgt ggagcggggc caacaaagcc tggtttggtc gcccgacgca
    32101 tccgggtaat gtcataggtc actggatttg cccgcagggt aatgagattc cagtggtcgc
    32161 cgttgccagc catgataccg ccagcgcggt tatcgcctcg ccgttaaacg gttcacgtgc
    32221 tgcttatctc tcttctggca cctggtcatt gatgggcttc gaaagccaga cgccatttac
    32281 caatgacacg gcactggcag ccaacatcac caatgaaggc ggggcggaag gtcgctatcg
    32341 ggtgctgaaa aatattatgg gcttatggct gcttcagcga gtgcttcagg agcggcaaat
    32401 caacgatctt ccggcgctta tctccgcgac acaggcactt ccggcttgcc gcttcattat
    32461 caatcccaat gacgatcgct ttattaatcc tgagacgatg tgcagcgaaa ttcaggctgc
    32521 gtgtcgggaa acggcgcaac cgatcccgga aagtgatgct gaactggcgc gctgcatttt
    32581 cgacagtctg gcgctgctgt atgccgatgt gttgcatgag ctggcgcagc tgcgcggtga
    32641 agatttctcg caactgcata ttgtcggcgg aggctgccag aacacgctgc tcaaccagct
    32701 atgcgccgat gcctgcggta ttcgggtgat cgccgggcct gttgaagcct cgacgctcgg
    32761 caatatcggc atccagttaa tgacgctgga tgaactcaac aatgtggatg atttccgtca
    32821 ggtcgtcagc accaccgcga atctgaccac ctttacccct aatcctgaca gtgaaattgc
    32881 ccactatgtg gcgcggattc actctacacg acagacaaag gagctttgcg catgaccact
    32941 caactggaac aggcctggga gctggcgaaa cagcgtttcg cggcagtagg gattgatgtc
    33001 gaggaggcgc tgcgccaact tgatcgttta cccgtttcaa tgcactgctg gcagggtgat
    33061 gatgtttccg gttttgaaaa cccggaaggt tcgctgaccg gagggattca ggctactggc
    33121 aattatccgg gcaaagcgcg taatgccagt gagctacgtg ccgatctgga acaggctatg
    33181 cggctgattc cggggccaaa acggcttaat ttacatgcca tctatctgga atcagacacg
    33241 ccagtctcgc gcgaccagat caaacctgag cacttcaaaa actgggttga atgggcgaaa
    33301 gccaatcagc tcggtctgga ttttaacccc tcctgtttct cgcatccgct aagcgccgat
    33361 ggctttacgc tttcccatgc cgacgacagc attcgccagt tctggattga tcactgcaag
    33421 gccagccgcc gcgtttcggc ctattttggt gagcaactcg gcacaccgtc ggtgatgaac
    33481 atctggatcc cggatggcat gaaagatatc accgttgacc gtctcgctcc gcgccagcgt
    33541 ctgctggcag ctctggatga ggtgatcagc gagaagctgg atccggcgca ccatatcgac
    33601 gccgttgaga gcaaattgtt tggcattggc gcagagagct acacggttgg ctccaatgag
    33661 ttttacatgg ggtatgccac cagccgccag actgcgctgt gcctggacgc cgggcatttc
    33721 cacccgactg aagtgatttc cgacaagatt tccgccgcca tgctgtatgt gccgcagttg
    33781 ctgctgcacg tcagccgtcc ggttcgctgg gacagcgatc acgtagtgct gctggatgat
    33841 gaaacccagg cgattgccag tgagattgtt cgtcacgatc tgtttgaccg ggtgcatatc
    33901 ggcctcgact tctttgatgc ctctatcaac cgtattgctg cgtggatcat tggtacacgc
    33961 aatatgaaaa aagccctgct gcgtgcgttg ctggaaccta ccgctgagct gcgcaagctg
    34021 gaagcggcgg gcgattacac tgcgcgtctg gcactgctgg aagagcagaa atcgttgccg
    34081 tggcaggcgg tctgggaaat gtattgccaa cgtcacgata cgccagcagg tagcgaatgg
    34141 ctggagagcg tgcgggcata tgagaaagaa actttgagtc gccgcgggta aacactgccg
    34201 gatgcggcac aagtgcctta tccggcctac aggccggcaa tagttgtagg cctgataaga
    34261 cgcgacagcg tcgcatcagg cattgattgc cggatgcggc acgagcgcct tatccggcct
    34321 acgggtcgtg catctgacaa caccgaattt acaggaacac agaacatgca aaacattacc
    34381 cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg atgcctggct gaaaggctgg
    34441 gacgagcgca acggcggcaa cctgacgctg cgcctggatg acgccgatat cgcgctttat
    34501 cacgacaact tccacccaca accgcgctat attccgctca gccaacccat gcctttactg
    34561 gcaaatacgc cgtttattgt caccggctcc ggcaaattct tccgtaacgt ccagctcgat
    34621 cctgcagcta acttaggcgt cgtaaaagtc gacagcgatg gcgcgggcta ccatattctc
    34681 tgggggttaa ccaacgaagc cgttccaact tctgaacttc cggctcactt tctctcccac
    34741 tgcgagcgca ttaaagccac caacggtaaa gatcgggtga tcatgcactg ccacgccacc
    34801 aacctgatcg ccctcaccta tgtacttgaa aacgacaccg cggtcttcac tcgccaactg
    34861 tgggaaggca gcaccgagtg tctggtggta ttcccggatg gcgttggcat tctgccgtgg
    34921 atggtacccg gcacggacga aatcggccag gcaactgccc aagagatgca aaaacattcg
    34981 ctggtgttgt ggcccttcca cggtgtgttc ggttccggac cgacgctgga tgaaaccttc
    35041 ggtttaatcg acaccgcaga aaaatcagcg gaagtgttag tgaaggtgta ttcgatgggc
    35101 ggcatgaaac agactatcag ccgtgaagag ttgatagcgc tcggccagcg tttcggcgtt
    35161 acaccactcg ccagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc
    35221 atgcaggtaa accccgacgc ccacgaagag tatcagcgtc ggcataatcc catctggccg
    35281 gaactggaag cagtgttgaa atctcacggt gcgcataact acgccattta cctcgacaaa
    35341 gcgcgtaatc tgctgtttgc cacggtagag attgaatctg aagaacggtg gaatgcggta
    35401 gccagcactg aaatctgcca acgttggtgg aaatatatga ccgatgttat gcccgctaac
    35461 ccggataaca gcccggtgag tagcgagctg caagaagtgt tttacctgcc ttaatgagtt
    35521 gcggtgccgg aaaatatcca gcaccgcgac ttcatcagaa gaagtattta aagcgaacgc
    35581 gtgcgccgta acggtcatca gtatcatcac cgtgtttttt atcgccgtcg ctatccagaa
    35641 tggagtaata cgtcccgagg taaatattaa agttctccat atccatcacg ttagcgaact
    35701 gataagacgc atgaatggtg tgaatggtgt acgtcccttc atcatcaatc cagcagtcgt
    35761 tatcacaaac tacaccgctg aattcatcaa tcttattgtg cgcgtagata taaccgagct
    35821 cgaaacgttt ccacagcgcg ttaatcccgg cagtgaaatc tttctcatta ttagcatcta
    35881 aataggcggt atttagatta accacaatgc cattgtccgg atccgttttc aggccgttcc
    35941 aggtcatgct cataccatag ccggtacgat cggactgatc gacaaaatta cccttgctat
    36001 cggtataacc ataagcatta tttaccacgt tcgcttccat cgctgcggaa acggtgaatt
    36061 cttccgtcgg cgaccaggca attactgggc gcagataagc aacatttttc tgctgctcca
    36121 tatcgcgtcc atgataatta ccgtcattat ataaagatgt tccgtcttca agtaacgtgt
    36181 ttaattcaaa ataccagtta tcgagttgtt tgctgacgag gaaattaccg ccagcgttag
    36241 agcgcccgcg gccctctttc atcatataga tataaccgct gccatcgtca taaagatcgt
    36301 tcgcagtatt accggaatgt tcaacaaagg tatcctgatt caatgggaac atatcgtagg
    36361 cttcaaaacg gcctactttg accttccagt cgttttcttt gccaaagaag aataccgcat
    36421 catcgatatt tactgaaccg tgcatatcac ccagcggttg tgcggagaac ccggcgaaat
    36481 aaccgttgtc catctttcgc ataccgtcaa aacctaacag aatacgacca ttgagattcc
    36541 actgttcatt agtgggatcg ctattcacat cagcgttggt cattgccagc aggccatgat
    36601 tactttccgc atccatatta aattctacgt cgccgtagat ttttaaatcg ccataacctg
    36661 aaagcaaaag attaggtgag gtggcatttt tctttgcaga ttccgccgtt gcctgtccat
    36721 ttaccggcgt attgccctga gcggccttaa tttcgcggat ctcagcggct tgttgtgtct
    36781 gcagctgttt aacctggctc tctgcttttg ccgcccgcat ttctgccgct tccaggcgtt
    36841 cttccagcaa tgccatgcgt tgttccagca ttaaatgctg tgtttttgcc ataccggtac
    36901 tacatgccaa taataaaccg atggctaaag ataatgtcga ttttttcatt atttacgttc
    36961 cctgaaataa aggagaccct acgctttcca aaaggaatac gtatatagag aaatataagg
    37021 tgtgcacttt cgaattttta atccaacttc agcaggccga tggatagtat cccgccgcat
    37081 aatatgagta aatccgctga tttcctggaa cgtgaggaat ataacaatag tgccgttttt
    37141 aactaaatat cgtaaaaaat attagatatg attcaagctg gctcataagc cacaaatata
    37201 aataacgttt agacaaatta tctgagacat ttaaacattc acaaaatcat taaataataa
    37261 ctctttattc acaagattta ttttacctac taatatgtgc cttacgccgc aaaatttatc
    37321 cttcactctg ctttatattg tcacgacttc acgacatatc cgcgctcatc acaaacccgc
    37381 cccgctatta caattaactg aaatattttt tcatggagtc cgattaatgg cagctcttac
    37441 tgcaagctgt attgacctga atattcaggg caatggcgct tattccgttc tgaagcagtt
    37501 ggcgacaatg gcgttacaaa acggttttat caccgactca caccagttcc tgcaaaccct
    37561 gctgctgcgc gaaaaaatgc actccaccgg ttttggttct ggtgtcgccg tgccgcacgg
    37621 taaaagcacc tgcgttaaac aaccgttcgt gttattcgcc cgcaaagcgc aggctattga
    37681 ctggaaagcc agcgacggcg aagacgtcaa ttgctggatc tgcctcggcg taccgcaaag
    37741 tggcgaagag gatcaggtca aaatcatcgg cacactgtgt cgcaaaatta ttcaccagga
    37801 atttattcat caactgcaac agggcgatac cgaccaggtg cttgccttgt taaatcaaac
    37861 cctcagctca taaggaagtg gcgatggagt catccttacg tattgtcgcg atcaccaact
    37921 gccccgccgg gatcgctcac acctacatgg tggcggaagc cctggaacag aaagcccgtt
    37981 ctctcggcca taccattaaa gtggaaactc aagggtccag cggcgttgaa aaccgcttat
    38041 ccagcgaaga gattgccgct gccgattacg tgattctcgc caccgggcgt ggcctgagcg
    38101 gtgacgatcg cgcacggttt gccgggaaaa aagtttatga gattgccatc tcccaggcgc
    38161 tgaaaaatat cgatcagatt ttcagtgaat taccgacaaa ttcgcagctt tttgccgcag
    38221 atagcggcgt gaagctaggc aaacaggaag tacaaagcgg cagcgtgatg agccacctga
    38281 tggcgggcgt ctctgcggcg ctgccgtttg tcatcggcgg tggtatcctg gtggcgcttg
    38341 ccaacatgct ggtgcagttc ggtttgccgt ataccgatat gtcgaaaggc gcgccgtcgt
    38401 ttacctgggt agttgaatcc atcggttacc ttggcttcac ctttatgatc cccatcatgg
    38461 gcgcttatat tgcctcgtcg attgccgata aaccggcttt cgctccggcg tttctggttt
    38521 gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga ttcctcggcg
    38581 cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgtaaa gtccgtcttg
    38641 gcaaagcatt acaaccgctg ctcggctcca tgcttatccc attcgttact ctgctggttt
    38701 ttggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg ggcgggctac
    38761 tgcacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt ctggtcggtg
    38821 cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc ttctgcttct
    38881 cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc cccgtcgcgg
    38941 cgggtcttgc gacgttcatt gcgcccaaac tgtttacccg acaggaaaaa gaggctgcca
    39001 gtagtgccat tgtggtcggt gccacagtcg caaccgaacc ggctattcct tacgctctgg
    39061 cagctccgtt gccgatgatt accgccaata cgctggcggg cggcattacc ggcgtgctgg
    39121 tgatcgcttt cggaatcaaa cgtctggcac ctggtttggg tatctttgac ccgctgatcg
    39181 gcctgatgtc gccggtaggt tcgttttatc tggtgctggc gattggcctc gcgctgaaca
    39241 tttcattcat tatcgtcctg aaaggattgt ggttacgccg caaagcgaaa gccgcgcaac
    39301 aggagcttgt ccatgaacat tgagttactg caacagttgt gcgaagccag cgccgtcagc
    39361 ggcgatgaac aggaagttcg cgacattctg ataaacacgc tggaaccttg cgttaatgag
    39421 atcacctttg atggtctggg cagctttgtt gcccgtaagg gaaataaagg tccaaaagtt
    39481 gccgttgtcg ggcatatgga tgaagtcggc tttatggtca cccacatcga cgagagcggt
    39541 tttctgcgct ttaccaccat tggcggctgg tggaatcagt cgatgctcaa ccaccgggta
    39601 acaatacgca cacacaaggg agtgaaaatc cctggtgtga ttggttccgt agcgcctcat
    39661 gcgttaacgg aaaagcaaaa gcaacaaccg ctgtcatttg atgagatgtt cattgatatt
    39721 ggcgcgaaca gtcgcgaaga agcgaaaaaa cgcggtgcgg aaattggcga ttttattagc
    39781 ccggaagcca attttgcctg ctggggcgaa gataaaatag tcggcaaggc gctggataat
    39841 cgcatcggct gcgcgatgat ggctgagcta ctacagacag taaataaccc aggaattacg
    39901 ctgtacggcg tcggcagcgt ggaagaagaa gttgggctac gcggggcaca aacctcggct
    39961 gaacacacta aaccggatgt ggtgatcgtg ctggataccg ccgtcgcggg cgatgttccg
    40021 ggcattgata acattaaata cccgctgaaa ctgggcaacg ggccggggct gatgctgttt
    40081 gacaagcgct acttacccaa ccagaaactg gtggcggcgt taaaaaactg tgccgcacat
    40141 aacggtttac cgctgcaatt ttccaccatg aaaaccggag cgacggatgg cgggcgctac
    40201 aacgtaatgg gcggagggcg tccggttgtc gcgctgtgtc tgccaactcg ttatctgcac
    40261 gctaacagcg gtatgatttc aaaagccgat tatgatgctc tgctcacgct gatacgggat
    40321 tttctgacga ccttaactgc ggagaaagtc aacgcgttta gccagttccg tcaggtggat
    40381 taatgctaaa cgaacgccag ttaaagattg tcgatctgct ggagcaacag ccgcgcacgc
    40441 ctggcgagct ggcgcaacag actggcgttt caggcaggac catcctgcgt gatattgact
    40501 atctcaactt tacccttaac ggcaaagctc gtatctctgc cagtggcagt gcgggctatc
    40561 agttggaaat cttcgagcgc cgcagctttt ttcagttgct gcaaaagcac gataacgacg
    40621 atcggctgct ggcgctgtta ttactgaata cctttactcc ccgtgcgcaa ctcgcctcgg
    40681 cgcttaattt gccagaaact tgggtcgcag agcgtctgcc ccggttaaaa cagcgttatg
    40741 aacgcacttg ttgcctggcc agtcgtcccg gtttgggcca tttcattgat gaaacagaag
    40801 agaaacgcgt tatcttgctg gcgaacttgc tacgcaaaga tccgttttta atacctttgg
    40861 ctggcgtaac acgagacaac cttcagcatt tatccacggc ctgcgacaac caacaccgct
    40921 ggccgctcat gcagggggat tatctctcca gcctgattct ggcgatttac gccctgcgta
    40981 atcaactgac cgatgagtgg ccgcaatatc ccggtgacga gataaaacaa atcgttgaac
    41041 ataacggtct gtttcttggt gataacgctg taagaaccct gacaggtttg atagagaaac
    41101 agcatcagca agcgcaggta atttctgccg atcatgtgct ggggttgctg caaagggtgc
    41161 caggtctcgc gtcattgaat attattgata cgcagctggt tgagaacatt accggtcatt
    41221 tattacgttg ccttgccgca ccagtgtgga ttgctgagca tcgccaaagc agcatgaata
    41281 acctgaaagc cgcctggcct gcggcctttg atatgagtct gcactttatt acgctactgc
    41341 gtgaacagct cgatattccc cttttcgaca gcgatctgat cggtttgtat tttgcctgtg
    41401 cgctggaacg acatcaaaac gaacgccagc cgatcatttt gctctcggac cagaacgcga
    41461 ttgccactat taatcagctc gccattgagc gcgatgtttt acattgtcgg gtgattattg
    41521 cccgtagctt aagcgaactt gttgccattc gcgaagagat tgagccgtta ttgatcatta
    41581 acaacagcca ttatttactg gatgacgcgg taaataatta catcaccgta aaaaatatca
    41641 ttacggctgc cggtatcgaa caaataaaac attttctggc gacggcattt attcgccagc
    41701 aaccggagcg ttttttctct gcccccggaa gttttcatta ttcgaatgta cgcggtgaaa
    41761 gctggcaaca tattacccgg caaatttgtg cgcaattagt cgcacaacac catattaccg
    41821 ccgatgaagc gcaacggatc atcgcccgcg aaggcgaagg tgaaaacctg attgttaatc
    41881 gcctcgccat cccacattgc tggagcgaac aggagcgacg ttttcgtgga ttttttatta
    41941 ccctcgccca accagttgag gtgaataacg aagtcattaa ccatgtcttg atcgcctgcg
    42001 ccgccgccga tgcgcgtcat gagctaaaaa tatttagcta tctggcaagc gtattgtgtc
    42061 agcatccggc agaagttatt gccgggctaa caggatatga ggcatttatg gagttacttc
    42121 acaaggggtg aactgtgtcg gatgcgccgc atccgacact gcatttatca tcaaaaacgt
    42181 ttaacgcagg ctattatagt catcaaccat ttcgttgtac tcgcgtaacg ctcgtggaaa
    42241 tgagtcttcg accatccagc ttgtgtttgc atcctgaaga tgctctttat cccagtcaga
    42301 gtacgggact ttatctctga cgcggcgaac atatttttta gtctcgagtt gatactggtc
    42361 tgccgaatta atgaacgaga aattcatgcc gcctttatcc gcttctttgg cctgcgccac
    42421 cagcgtttcc agttcagaca ctttcttcat tgccgcatca acatcaaact tctcttgcga
    42481 tatcaggtta ttaatttgtt tcgctgaaat catgactgcc agagaatagt agtggaatgt
    42541 tttcccttct ctttcttcaa tatttttcag ttccgcaagc tggcgcttat cattaatttc
    42601 ctgaatcgcc gcatgataag attctgctac aggttcaaag gcttccagat tttttaagaa
    42661 tgtctggtgc agcgttttcc ctttcgcaaa cgcatcgtct ttataatttt cctgggtgta
    42721 atatttatcc atttcgttaa tggtattacc caacgccacg gcagcatcga tataactcac
    42781 cgccacgcca tcaattggtt gcagcgcagg cgttaacgcc acagcacttt ttacgccttt
    42841 ttcacactca gcgaggttgg attcactaat gccgtagatg ccataaacag tactctcttt
    42901 accggtaggc ccctgtttaa aatctttcag ccagtcagca taacgcgcca gactgcgctg
    42961 taccgggatc tgtaacttgt tgtagcactt gatatagaca tccatttttt cgtcgaaaac
    43021 cgcttgttca tcgaccagcg gttgcgcagg tgtttcaggt ttagcatctg cttttgcaac
    43081 gggagcttca gtaggtttcg cttcaggagc tggtgcggca ggttgactat tggcaggcag
    43141 gggcgtttct gtttcggctt ttttatcatc acaacccgtc agaccgaggg ccatgatggc
    43201 gactataata gcggaagata
//
LOCUS       DABSYT020000028        43005 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.028, whole genome shotgun sequence.
ACCESSION   DABSYT020000028 DABSYT020000000
VERSION     DABSYT020000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 43005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.028"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..339
                     /locus_tag="ID725_RS09715"
                     /old_locus_tag="pgaptmp_001943"
     CDS             <1..339
                     /locus_tag="ID725_RS09715"
                     /old_locus_tag="pgaptmp_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HGV7850554.1"
                     /translation="KKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYS
                     DDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKECSSYLGTPNNTYLKYNPKAKGN
                     GVTIIRQGSN"
     gene            341..663
                     /locus_tag="ID725_RS09720"
                     /old_locus_tag="pgaptmp_001944"
                     /pseudo
     CDS             341..663
                     /locus_tag="ID725_RS09720"
                     /old_locus_tag="pgaptmp_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
     gene            complement(750..1425)
                     /locus_tag="ID725_RS09725"
                     /old_locus_tag="pgaptmp_001945"
                     /pseudo
     CDS             complement(750..1425)
                     /locus_tag="ID725_RS09725"
                     /old_locus_tag="pgaptmp_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1570..2178)
                     /gene="yiiX"
                     /locus_tag="ID725_RS09730"
                     /old_locus_tag="pgaptmp_001946"
     CDS             complement(1570..2178)
                     /gene="yiiX"
                     /locus_tag="ID725_RS09730"
                     /old_locus_tag="pgaptmp_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGV7850555.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2238..2555)
                     /gene="metJ"
                     /locus_tag="ID725_RS09735"
                     /old_locus_tag="pgaptmp_001947"
     CDS             complement(2238..2555)
                     /gene="metJ"
                     /locus_tag="ID725_RS09735"
                     /old_locus_tag="pgaptmp_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGV7850556.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2832..3992
                     /gene="metB"
                     /locus_tag="ID725_RS09740"
                     /old_locus_tag="pgaptmp_001948"
     CDS             2832..3992
                     /gene="metB"
                     /locus_tag="ID725_RS09740"
                     /old_locus_tag="pgaptmp_001948"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGV7850557.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3995..6427
                     /gene="metL"
                     /locus_tag="ID725_RS09745"
                     /old_locus_tag="pgaptmp_001949"
     CDS             3995..6427
                     /gene="metL"
                     /locus_tag="ID725_RS09745"
                     /old_locus_tag="pgaptmp_001949"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGV7850558.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6566..6700)
                     /locus_tag="ID725_RS09750"
                     /old_locus_tag="pgaptmp_001950"
     CDS             complement(6566..6700)
                     /locus_tag="ID725_RS09750"
                     /old_locus_tag="pgaptmp_001950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850559.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6776..7666
                     /gene="metF"
                     /locus_tag="ID725_RS09755"
                     /old_locus_tag="pgaptmp_001951"
     CDS             6776..7666
                     /gene="metF"
                     /locus_tag="ID725_RS09755"
                     /old_locus_tag="pgaptmp_001951"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGV7850560.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7995..10175
                     /gene="katG"
                     /locus_tag="ID725_RS09760"
                     /old_locus_tag="pgaptmp_001952"
     CDS             7995..10175
                     /gene="katG"
                     /locus_tag="ID725_RS09760"
                     /old_locus_tag="pgaptmp_001952"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGV7850561.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10269..11174
                     /gene="yijE"
                     /locus_tag="ID725_RS09765"
                     /old_locus_tag="pgaptmp_001953"
     CDS             10269..11174
                     /gene="yijE"
                     /locus_tag="ID725_RS09765"
                     /old_locus_tag="pgaptmp_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGV7850562.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(11201..11818)
                     /gene="yijF"
                     /locus_tag="ID725_RS09770"
                     /old_locus_tag="pgaptmp_001954"
     CDS             complement(11201..11818)
                     /gene="yijF"
                     /locus_tag="ID725_RS09770"
                     /old_locus_tag="pgaptmp_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGV7850563.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12092..13195)
                     /gene="gldA"
                     /locus_tag="ID725_RS09775"
                     /old_locus_tag="pgaptmp_001955"
     CDS             complement(12092..13195)
                     /gene="gldA"
                     /locus_tag="ID725_RS09775"
                     /old_locus_tag="pgaptmp_001955"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGV7850564.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13206..13868)
                     /gene="fsa"
                     /locus_tag="ID725_RS09780"
                     /old_locus_tag="pgaptmp_001956"
     CDS             complement(13206..13868)
                     /gene="fsa"
                     /locus_tag="ID725_RS09780"
                     /old_locus_tag="pgaptmp_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGV7850565.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13880..16381)
                     /gene="ptsP"
                     /locus_tag="ID725_RS09785"
                     /old_locus_tag="pgaptmp_001957"
     CDS             complement(13880..16381)
                     /gene="ptsP"
                     /locus_tag="ID725_RS09785"
                     /old_locus_tag="pgaptmp_001957"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGV7850566.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16690..17769
                     /gene="frwC"
                     /locus_tag="ID725_RS09790"
                     /old_locus_tag="pgaptmp_001958"
     CDS             16690..17769
                     /gene="frwC"
                     /locus_tag="ID725_RS09790"
                     /old_locus_tag="pgaptmp_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGV7850567.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17784..18104
                     /gene="frwB"
                     /locus_tag="ID725_RS09795"
                     /old_locus_tag="pgaptmp_001959"
     CDS             17784..18104
                     /gene="frwB"
                     /locus_tag="ID725_RS09795"
                     /old_locus_tag="pgaptmp_001959"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGV7850568.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            18155..20452
                     /gene="pflD"
                     /locus_tag="ID725_RS09800"
                     /old_locus_tag="pgaptmp_001960"
     CDS             18155..20452
                     /gene="pflD"
                     /locus_tag="ID725_RS09800"
                     /old_locus_tag="pgaptmp_001960"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGV7850569.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            20418..21296
                     /gene="pflC"
                     /locus_tag="ID725_RS09805"
                     /old_locus_tag="pgaptmp_001961"
     CDS             20418..21296
                     /gene="pflC"
                     /locus_tag="ID725_RS09805"
                     /old_locus_tag="pgaptmp_001961"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGV7850570.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21298..21639
                     /gene="frwD"
                     /locus_tag="ID725_RS09810"
                     /old_locus_tag="pgaptmp_001962"
     CDS             21298..21639
                     /gene="frwD"
                     /locus_tag="ID725_RS09810"
                     /old_locus_tag="pgaptmp_001962"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGV7850571.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21626..22477)
                     /gene="yijO"
                     /locus_tag="ID725_RS09815"
                     /old_locus_tag="pgaptmp_001963"
     CDS             complement(21626..22477)
                     /gene="yijO"
                     /locus_tag="ID725_RS09815"
                     /old_locus_tag="pgaptmp_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7850572.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22692..24425)
                     /gene="eptC"
                     /locus_tag="ID725_RS09820"
                     /old_locus_tag="pgaptmp_001964"
     CDS             complement(22692..24425)
                     /gene="eptC"
                     /locus_tag="ID725_RS09820"
                     /old_locus_tag="pgaptmp_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGV7850573.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24608..27259)
                     /gene="ppc"
                     /locus_tag="ID725_RS09825"
                     /old_locus_tag="pgaptmp_001965"
     CDS             complement(24608..27259)
                     /gene="ppc"
                     /locus_tag="ID725_RS09825"
                     /old_locus_tag="pgaptmp_001965"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGV7850574.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27611..28762)
                     /gene="argE"
                     /locus_tag="ID725_RS09830"
                     /old_locus_tag="pgaptmp_001966"
     CDS             complement(27611..28762)
                     /gene="argE"
                     /locus_tag="ID725_RS09830"
                     /old_locus_tag="pgaptmp_001966"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGV7850575.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28916..29920
                     /gene="argC"
                     /locus_tag="ID725_RS09835"
                     /old_locus_tag="pgaptmp_001967"
     CDS             28916..29920
                     /gene="argC"
                     /locus_tag="ID725_RS09835"
                     /old_locus_tag="pgaptmp_001967"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGV7850576.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29928..30704
                     /gene="argB"
                     /locus_tag="ID725_RS09840"
                     /old_locus_tag="pgaptmp_001968"
     CDS             29928..30704
                     /gene="argB"
                     /locus_tag="ID725_RS09840"
                     /old_locus_tag="pgaptmp_001968"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGV7850577.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30765..32138
                     /gene="argH"
                     /locus_tag="ID725_RS09845"
                     /old_locus_tag="pgaptmp_001969"
     CDS             30765..32138
                     /gene="argH"
                     /locus_tag="ID725_RS09845"
                     /old_locus_tag="pgaptmp_001969"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGV7850578.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            32405..33322
                     /gene="oxyR"
                     /locus_tag="ID725_RS09850"
                     /old_locus_tag="pgaptmp_001970"
     CDS             32405..33322
                     /gene="oxyR"
                     /locus_tag="ID725_RS09850"
                     /old_locus_tag="pgaptmp_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGV7850579.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(33305..34705)
                     /gene="sthA"
                     /locus_tag="ID725_RS09855"
                     /old_locus_tag="pgaptmp_001971"
     CDS             complement(33305..34705)
                     /gene="sthA"
                     /locus_tag="ID725_RS09855"
                     /old_locus_tag="pgaptmp_001971"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGV7850580.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35035..36201
                     /locus_tag="ID725_RS09860"
                     /old_locus_tag="pgaptmp_001972"
     CDS             35035..36201
                     /locus_tag="ID725_RS09860"
                     /old_locus_tag="pgaptmp_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HGV7850581.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            36244..37560
                     /locus_tag="ID725_RS09865"
                     /old_locus_tag="pgaptmp_001973"
     CDS             36244..37560
                     /locus_tag="ID725_RS09865"
                     /old_locus_tag="pgaptmp_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HGV7850582.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            37664..38314
                     /gene="fabR"
                     /locus_tag="ID725_RS09870"
                     /old_locus_tag="pgaptmp_001974"
     CDS             37664..38314
                     /gene="fabR"
                     /locus_tag="ID725_RS09870"
                     /old_locus_tag="pgaptmp_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGV7850583.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            38314..38673
                     /gene="yijD"
                     /locus_tag="ID725_RS09875"
                     /old_locus_tag="pgaptmp_001975"
     CDS             38314..38673
                     /gene="yijD"
                     /locus_tag="ID725_RS09875"
                     /old_locus_tag="pgaptmp_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGV7850584.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38713..39813)
                     /gene="trmA"
                     /locus_tag="ID725_RS09880"
                     /old_locus_tag="pgaptmp_001976"
     CDS             complement(38713..39813)
                     /gene="trmA"
                     /locus_tag="ID725_RS09880"
                     /old_locus_tag="pgaptmp_001976"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGV7850585.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECSVLLTAK"
     regulatory      39927..40117
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            40182..42026
                     /gene="btuB"
                     /locus_tag="ID725_RS09885"
                     /old_locus_tag="pgaptmp_001977"
     CDS             40182..42026
                     /gene="btuB"
                     /locus_tag="ID725_RS09885"
                     /old_locus_tag="pgaptmp_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGV7850586.1"
                     /translation="MIKKASLLTACSVTAFSSWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            41971..42828
                     /gene="murI"
                     /locus_tag="ID725_RS09890"
                     /old_locus_tag="pgaptmp_001978"
     CDS             41971..42828
                     /gene="murI"
                     /locus_tag="ID725_RS09890"
                     /old_locus_tag="pgaptmp_001978"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGV7850587.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9861 a        10639 c        11872 g        10633 t
ORIGIN      
        1 aagaagcaag gtgaatattt ccccatgaat ccgataagtt caaatgataa tgatagtgta
       61 gacttgcgaa atataaaatg ccctgaaggt tcagagagag taggcgatta tcacactcac
      121 ggtttttact ctgacgataa aggaaataaa gtaacaaaag aaaatgatgt ttatgatagt
      181 ctaaattttt caagcaaaga tttaacgaat tcttatatga atggaatggg aaaaaaagaa
      241 tgcagtagtt acttgggaac accaaataac acctatctaa aatataatcc caaagctaaa
      301 gggaatggag ttacaattat caggcaaggg agtaattaaa atgaaggcaa tacaatgttg
      361 ttatttcctt gctttagtct ccagtcattt aatggctgga gttatgagtg atgacaatat
      421 atttttaagt aattcggatt tagctgcttt atattgtgtg gctaaaatat ttccagatgg
      481 agaaaatatt ttaatatata aagtaaatat aattaatgct agttcaaaaa agatgtattt
      541 ttttatgaaa gcccaacatc aacccacaat gacataatgg ggggcggggg aatgtcatat
      601 gcctatgaat atgatgtaaa atataatata tgtcatttga gaaaaaagag cagaatgagg
      661 taaaaatctt tggctgtgta ttcagtctgg taatcatcca aggaaagctc atcagaaggg
      721 tgatgactcc aattagtaga acatatattt tcgataaaag caccgatgac tttttcatga
      781 agttctacag aactcgagaa acagatggtt ctgcgggcca gacgtttgat tcgggttctc
      841 agggtcagat tgttacgctc aatgtgctga gtaaaaatct taccagtcag atgcttctcc
      901 tttggtaact ctctagcgta actaccccat tcgtcagtgg tcaacatgcc gatggtaaaa
      961 gggtaagcag cgccagcaat tcacgacagg tttcatcagt ccgggggcca aaagtgtagg
     1021 ccagaacacc accagttttc gtgttgtaca cgtaccagag ccagtgctgt cgcgctttac
     1081 tgccgacaaa gctccattgc tgactaagtt tgcagatgag tgccacatca gcatgagcga
     1141 ccggggatga cgttattctt cgtggcgaga gttttttaaa gttcggatga ctgtattaat
     1201 gccgattttc agcgtacggg cagtatagcg aaccccggca ccgttgaaag ccatttcagc
     1261 gatttggtct ttaactcccg gtttacgggg ctcgtaacag taactgagct gaaatacccg
     1321 gtgacagtca cggccgcgga atcgatcatg tcttttgggg ttctgcccgc gacggtaaac
     1381 ctgaacggac tggcaacggg gacaatgaag gttaactgtg accatgaaac atccttaaaa
     1441 gcgagtatta tatccttaat tcaactaatt ggaggcatca ccgtttaccg gagcgacagc
     1501 ttgcggaaag gtcgtgcaac tacaaagcaa caaacagact acggcgggaa ttattttttt
     1561 catcatccat taccacgaaa acagcggcca ttctttggca accgtggtga gttgtggcgc
     1621 atcgaaaacg gcttgtggtg agaccaccgt ttcctcaagc ggaatatttt tgccgtagcg
     1681 ttctttgagc ttcgcctgga ccagcgggtt gctgagatca aactctttca acttctgctg
     1741 ttcgcccaca cgcattccca gcgcattctg ataaaccttc cacaccactt ccgaacagta
     1801 ctggcgatcg tcgctccatg agaagctgaa atcgtacggt ttgccgagat aacgtttcgc
     1861 cgtttgcgcc agtttttgct gttgttcaac actcagtccg ccttcaacgc ggcgaacaac
     1921 gtatttgccc ttttcaccat gggcaatcca ctgcttgagc ggggtgtatt tcaccgggcc
     1981 gaccgcttca aaaacgtagg gctttttgtt acgcatcacc agcataccgg tgtggctgta
     2041 atcggaatgg gtcgccagtt ggatcgcttt actttgcgat gagcgagaga tttgaaagat
     2101 gatgtcgcca gtttgcggct gccaggcaaa ggcaggcaca gaaaccagca ggctgaggat
     2161 cagtagcctg attttcatcg ttaaacgtcc ttgtatgaaa aaagcggctg gttaactccg
     2221 cgccgctctt ttccgcttta gtattcccac gtctccgggt taatccccat ctcacgcatg
     2281 atctcttttg ccgcttccgg gatttcgtcg ctgcgctctt tacgcagatc ggcatcatcc
     2341 ggcaaaggtt gcccggtaaa ggcatgcaga aacgcttcgc acagcagctc gctgttggta
     2401 gcgtgacgca ggttgttcac ctgacgacgc gtgcgttcat cggtgaggat ttttaacacc
     2461 ttaagaggaa tggaaaccgt aatctttttg acttgttcac tcttcttgcc gtgctcagcg
     2521 tatgggctga tatattcgcc gctccattca gccatgagat acttaatcct cttcgtcaat
     2581 aaattgagac cagaccacac aatttatgtg ggtactgacc gtaaacccgc atagtttacc
     2641 gtacaggcgt taccgtgaca tcgtgtaatg cacctgtcgg cgtgataatg catataattt
     2701 taacggctat ttgggatttg ctcaatctat acgcaaagaa gtttagatgt ccagatgtat
     2761 tgacgtccat taacacagtg tttactctgg tgcctgacat ttcaccgacc aagcccaggg
     2821 aacttcatca catgacgcgt aaacaggcca ccatcgcagt gcgtagcggg ttaaatgacg
     2881 acgaacagta tggttgcgtt gtccctccca tccatctttc cagcacctat aactttaccg
     2941 gatttaatga accgcgcgcg catgattact cgcgtcgcgg caacccaacg cgcgatgtgg
     3001 ttcagcgtgc gctggcagaa ctggaaggcg gtgctggtgc agtacttact aataccggca
     3061 tgtccgcgat tcatctggta acgaccgtct ttttgaaacc tggcgatctg ctggttgcgc
     3121 cgcacgactg ctacggcggt agctatcgcc tgttcgacag cctggcgaaa cgcggttgct
     3181 atcgcgtgtt gtttgttgat caaggcgatg aacaggcatt acgggcagcg ctggcagaaa
     3241 accccaaact ggtactggta gaaagcccaa gtaatccatt gttacgcgtc gtggatattg
     3301 cgaaaatctg ccatctggca agggaagtcg gggcggtgag cgtggtggat aacaccttct
     3361 taagcccggc attacaaaat ccgctggcat taggtgccga tctggtgttg cattcatgta
     3421 cgaaatatct gaacggtcac tctgacgtgg tggctggcgt ggtgattgct aaagacccgg
     3481 acgttgtcac tgaacttgcc tggtgggcaa acaatattgg cgtgacgggc ggcgcgtttg
     3541 acagctatct gctgctacgc gggttgcgaa cgctggtgcc gcgtatggag ctggcgcagc
     3601 gcaacgcgaa ggcgattgtg aaatatctgc aaacccagcc gttggtgaaa aaactgtatc
     3661 acccgtcgct gccggaaaat caggggcatg aaattgccgc gcgtcagcaa aaaggctttg
     3721 gcgcaatgtt gagttttgaa ctggatggcg atgagcagac gctgcgccgt ttcctgggcg
     3781 ggctgtcgtt atttacgctg gcggaatcat tagggggagt ggaaagttta atctctcacg
     3841 ccgcaaccat gacacatgca ggcatggcac cagaagcgcg tgctgccgcc gggatctccg
     3901 agacgctgct gcgtatctcc accggtattg aagatggcga agatttaatt gccgacctgg
     3961 aaaatggctt ccgggctgca aacaaggggt aaaaatgagt gtgattgcgc aggcaggggc
     4021 gaaaggtcgt cagctgcata aatttggtgg cagtagtctg gctgatgtga agtgttattt
     4081 gcgtgtcgcg ggcattatgg cggagtactc tcagcctgac gatatgatgg tggtttccgc
     4141 cgctggtagc accactaacc agttgattaa ctggttgaaa ctaagccaga ccgatcgtct
     4201 ctctgcgcat caggttcaac aaacgctgcg tcgctatcag tgcgatctga ttagcggtct
     4261 gctacccgcc gaagaagccg atagcctcat tagcgctttt gtcagcgatc ttgagcgcct
     4321 ggcggcgctg ctcgacagcg gtattaacga cgcagtgtat gcggaagtgg tgggccacgg
     4381 cgaagtgtgg tcggcgcgcc tgatgtctgc ggtgcttaat caacaagggc tgccagcggc
     4441 ctggcttgat gcccgcgagt ttttacgcgc tgaacgcgct gcgcaaccgc aggttgatga
     4501 agggctttct tacccgttgc tgcaacagct actggtacaa catccgggta aacgtctggt
     4561 ggtgaccggg tttatcagcc gtaacaacgc cggtgaaacg gtgctgctgg ggcgtaacgg
     4621 ttccgactat tccgcgacac aaatcggtgc gctggcgggt gtttctcgtg taaccatctg
     4681 gagcgacgtc gccggggtat acagtgccga tccgcgtaaa gtgaaagatg cctgtctgct
     4741 gccattgctg cgtctggatg aagccagcga actggcgcgt ctggcggctc ccgttcttca
     4801 cgcccgtact ttacagccag tatccggtag cgaaatcgac ctgcagctgc gctgtagcta
     4861 cacgccggat caaggctcca cgcgcattga acgcgtgctg gcctccggta ctggtgcgcg
     4921 tattgtcacc agccacgatg atgtctgctt gattgagttt caggtgccca ccagccagga
     4981 tttcaaactg gcgcataaag agatcgacca gatcctgaaa cgtgctcagg tgcgcccact
     5041 ggcggttggc gtgcataacg atcgccagtt gctgcaattt tgctacacct cagaagtggc
     5101 cgacagtgcg ctgaaaatcc tcgacgaagc gggattacct ggcgaactgc gcctgcgtca
     5161 ggggctggcg ctggtggcga tggtcggggc gggtgtcacc cgtaacccgc tgcattgcca
     5221 ccgcttctgg cagcaactga aaggccagcc ggtcgagttt acctggcagt ccgatgacgg
     5281 catcagcctg gtggcagtac tgcgcaccgg cccgaccgaa agcctgattc agggcctgca
     5341 tcagtccgtc ttccgcgcag aaaaacgcat cggcctggta ttgttcggca aaggcaatat
     5401 cggttcccgc tggctggaac tgttcgcccg cgagcagagc acgctttcgg cgcgtaccgg
     5461 ctttgaattt gtgctggcag gcgtggtgga cagccgccgc agtctgttga gttatgacgg
     5521 gctggacgcc agccgcgcgt tagccttctt caacgatgaa gcggttgagc aggatgaaga
     5581 gtcgttgttc ctgtggatgc gcgcccatcc gtatgatgat ttagtggtgc tggacgttac
     5641 cgccagccag cagctggctg accagtatct tgatttcgcc agccacggtt tccacgtcat
     5701 cagtgccaac aaactggcgg gagcaagcga cagcaataaa tatcgccaga tccacgacgc
     5761 cttcgaaaaa accgggcgtc actggctata caacgccacc gtcggtgcgg gcttgccgat
     5821 caaccatacc gtgcgcgatc tgatcgacag cggcgatact attttgtcga tcagtgggat
     5881 cttctccggc acgctctcct ggctgttcct gcaattcgac ggtagcgtgc cgtttaccga
     5941 gctggtggat caggcgtggc agcagggctt aaccgagcct gatccgcgtg acgacctctc
     6001 cggcaaagac gtaatgcgca agctggtgat tctggcgcgt gaagcgggtt acaacattga
     6061 accggaccag gtacgtgtgg aatcgctggt gcctgctcat tgcgaaggcg gcagcatcga
     6121 ccatttcttt gaaaatggcg atgaacttaa cgagcagatg gtgcaacggc tggaagcggc
     6181 ccgcgaaatg gggctggtgc tgcgctacgt ggcgcgtttc gatgccaacg gcaaagcgcg
     6241 tgtgggcgtg gaggcggtgc gtgaagacca tccgctggca tcactgctac cgtgcgataa
     6301 cgtctttgcc atcgaaagcc gctggtatcg cgacaacccg ctggtgatcc gtgggcctgg
     6361 cgcggggcgc gacgtcaccg ccggggcgat tcagtcggat atcaaccggc tggcacagtt
     6421 gttgtaattt cagaaattta ataatgcccg gtactcatgt tttcgggttt atggtttcta
     6481 atgaaatata ttgaattatc ataggattag gccggatgaa gcgtttacga cgaatccggc
     6541 aagaagcaat aagtacatgg ttagtttata tttgcagtcc ggtttgcttt gcataccgga
     6601 ttttcttttt cttaccatcc tgaagttttt tcatcttccc tgatttttcc tcaccatcat
     6661 tggtcatttt tcggttgacg cccttcggct tttccttcat ctttacatct ggacgtctaa
     6721 acggatggat gtgcacaaca caacatataa ctacaagcga ttgatgaggt aaggtatgaa
     6781 cttttttcac gccagccagc gggatgccct gaatcagagc ctggcagaag tccaggggca
     6841 gattaacgtt tcgttcgagt ttttcccgcc gcgtaccagt gaaatggagc agaccctgtg
     6901 gaactccatc gatcgcctta gcagcctgaa accgaagttt gtatcggtga cctatggcgc
     6961 gaactccggc gagcgcgacc gtacgcacag cattattaaa ggcattaaag atcgcactgg
     7021 tctggaagcg gcaccgcatc ttacttgcat tgatgcgacg cccgacgagt tgcgcaccat
     7081 tgcacgcgac tactggaata acggtattcg tcatatcgtg gcgctgcgtg gcgatctgcc
     7141 gccgggaagt ggtaagccag aaatgtatgc ttctgacctg gtgacgctat taaaagaagt
     7201 ggcagatttc gatatctccg tggcggcgta tccggaagtt cacccggaag caaaaagcgc
     7261 ccaggcggat ttgcttaacc tgaaacgcaa agtcgatgca ggagccaacc gcgcgattac
     7321 tcagttcttc tttgatgtcg aaagctacct gcgttttcgt gaccgctgtg tgtcggcggg
     7381 cattgatgtg gaaatcattc cgggcatatt gccggtatct aactttaaac aggcgaagaa
     7441 atttgccgac atgaccaacg tgcgtattcc ggcgtggatg gcgcaaatgt ttgacgggct
     7501 ggatgacgat gccgaaaccc gcaagctggt gggcgcgaat attgccatgg atatggtgaa
     7561 gattttaagc cgtgaaggag tgaaagattt ccacttctat acgcttaacc gcgctgaaat
     7621 gagttacgcg atttgccata cgctgggggt tcgacctggt ttataaatag tgtggctttt
     7681 atgaaaatca cacagtgatc acaaatttta aacagagcac aaaatgctgc ctcgaaatga
     7741 gggcgggaaa ataaggttat cagccttgtt ttctccctca ttacttgaag gatatgaagc
     7801 taaaaccctt ttttataaag catttgtccg aattcggaca taatcaaaaa agcttaatta
     7861 cgatcaattt gatctacatc tctttaacca acaatatgta agatctcaac tatcgcatcc
     7921 gtggattaat tcaattataa cttctctcta acgctgtgta tcgtaacggt aacactgtag
     7981 aggggagcac attgatgagc acgtcagacg atatccataa cacgacagcc actggcaaat
     8041 gcccgttcca tcagggcggt cacgaccaga gcgcgggggc gggcacaacc actcgcgact
     8101 ggtggccaaa tcaactccgt gttgacctgt taaaccaaca ttctaatcgt tctaacccgc
     8161 tgggtgagga ctttgactac cgcaaagaat tcagcaaatt agattactac ggcctgaaaa
     8221 aagatctgaa agccctgctg acagaatctc agccgtggtg gccagccgac tggggcagct
     8281 acgccggtct gtttattcgt atggcctggc acggcgcggg gacttaccga tcaatcgatg
     8341 gacgcggtgg cgcgggtcgt ggtcagcaac gttttgcacc gctgaactcc tggccggata
     8401 acgtaagcct cgataaagcg cgtcgcttgt tgtggccaat caaacagaaa tatggtcaga
     8461 aaatctcctg ggccgacctg tttatcctcg cgggtaacgt ggcgctggaa aactccggct
     8521 tccgtacctt cggttttggt gccggtcgtg aagacgtctg ggaaccggat ctggatgtta
     8581 actggggtga tgaaaaagcc tggctgactc accgtcatcc ggaagcgctg gcgaaagcac
     8641 cgctgggtgc aaccgagatg ggtctgattt acgttaaccc ggaaggcccg gatcacagcg
     8701 gcgaaccgct ttctgcggca gcagctatcc gcgcgacctt cggcaacatg ggtatgaacg
     8761 acgaagaaac cgtggcgctg attgcgggtg gtcatacgct gggtaaaacc cacggtgctg
     8821 gtccgacatc aaatgtaggt cctgatccag aagctgcgcc gattgaagaa caaggtttag
     8881 gttgggcgag cacttacggc agcggcgttg gcgcagatgc cattacctct ggtctggaag
     8941 tggtctggac ccagacgccg acccagtgga gcaactattt cttcgagaac ctgttcaagt
     9001 atgagtgggt acagacccgc agtccggctg gcgcaatcca gttcgaagcg gtagacgcac
     9061 cggaaattat cccggatccg ttcgatccgt cgaagaaacg taaaccgacg atgctggtga
     9121 ccgacctgac gctgcgtttt gatcctgagt tcgagaagat ctctcgtcgt ttcctcaacg
     9181 atccgcaggc gttcaacgaa gcctttgccc gtgcctggtt caaactgacg cacagggata
     9241 tggggccgaa atctcgttac atcgggccgg aagtaccgaa agaagatctg atctggcaag
     9301 atccgctgcc gcagccgatc tacaacccga ccgagcagga cattatcgat ctgaaattcg
     9361 cgattgcgga ttctggtctg tctgttagtg agctggtatc ggtggcctgg gcttctgctt
     9421 ctaccttccg tggtggcgac aaacgcggtg gtgccaacgg tgcgcgtctg gcattaatgc
     9481 cgcagcgcga ctgggatgtg aacgccgcag ccgttcgtgc tctgcctgtt ctggagaaaa
     9541 ttcagaaaga gtctggtaaa gcctcgctgg cggatatcat cgtgctggca ggtgtggttg
     9601 gcgttgagaa agccgcaagc gccgctggtt tgagcattca tgtaccgttt gcgccgggtc
     9661 gcgttgatgc gcgtcaggat cagactgaca ttgagatgtt cgaactgctg gagccaattg
     9721 ctgacggttt ccgtaactat cgcgctcgtc tggatgtttc caccaccgag tcactgctga
     9781 ttgataaagc ccagcaactg accctgaccg cgccggaaat gaccgcgctg gtgggcggga
     9841 tgcgtgtact gggtgccaac ttcgatggca gcaaaaacgg cgtcttcact gaccgcgttg
     9901 gcgtattgag caatgacttc ttcgtgaact tgctggatat gcgttacgag tggaaagcga
     9961 ccgacgaatc gaaagagctg ttcgaaggcc gtgaccgcga aaccggcgaa gtgaaataca
    10021 ctgccagccg tgcggatctg gtatttggtt ctaactctgt cctgcgtgcg gtggctgaag
    10081 tttacgccag cagcgatgct cacgagaagt ttgttaaaga cttcgtggcg gcatgggtga
    10141 aagtgatgaa cctcgaccgt ttcgacctgc tgtaatctga ccttcttcag cgactgcctt
    10201 tcaggcagtc gctgaagttt ctttaccggc gtatagtgtc cacaggaaaa ctacacactg
    10261 gatctctcat gtctgccgca ggaaagagca acccactggc aatcagtggc ctggttgtgc
    10321 tcacacttat ctggagttat agctggattt tcatgaagca agtcaccagc tacatcggtg
    10381 ccttcgactt taccgcctta cgctgcattt tcggcgctct cgttttattc atcgtccttt
    10441 tattacgtgg tcgcggaatg cgcccgacac cgtttaaata caccttagcc attgccctgt
    10501 tacaaacctg cgggatggtt ggcctggcgc agtgggcgct ggtcagcggc ggagcgggga
    10561 aggtggcgat cctgagctat accatgccgt tctgggtggt gattttcgcc gcggtgtttc
    10621 tcggtgaacg cctgcgacgt gggcaatatt tcgcgattct gattgccgct ttcggcttat
    10681 ttttggtgtt gcagccgtgg caactcgatt tctcttcgat gaaaagtgcc atgctggcaa
    10741 tcctctccgg tgttagttgg ggggcgagcg cgattgttgc taaacgtctg tatgcccgtc
    10801 atccgcgcgt ggatttattg tcgttaacat cctggcagat gctctatgcg gcgctggtga
    10861 tgagtgtggt cgctttactg gtgccgcaac gtgaaattga ctggcagccc actgtgttct
    10921 gggctctggc ctacagtgcg attctggcga cggcgttggc gtggagctta tggctgtttg
    10981 tgctgaaaaa cttgcctgcc agtattgcca gcttaagcac actggccgtt cccgtttgcg
    11041 gcgtactctt ttcctggtgg ctgctcggtg agaatccggg ggccgttgaa ggtagcggta
    11101 ttgtgctgat tgtgctggca ctggcgctgg tgagccgtaa gaaaaaagaa gccgttagtg
    11161 taaaaaggat ctgaattttt tcttcatgtg gggcgatcgc ttatttaaca aaataacgat
    11221 aatgccccac catgcgccag ctaaacagca catcttcttc ctgcgcacct gcgccaatgt
    11281 tatgtatcac cagcggcgta ccgtcgcggg cgaagccatc tgaaaccacc ccaatatgtg
    11341 ccagcccgtt atctaatcgc caggagacaa tatcgcccgc ttgatagtca ctggggttct
    11401 tgctgatggg gcgtgtttta tcgtggcggg taaaccaggt ttccagatta ggcacccgac
    11461 ggtgatcgat gttgctgtcc gggcgcttta actgccattt ttgcgggtac tcggcaaaat
    11521 tcttcgccat atcttcgtgc accagtttct gcaaatcgac cttctggctg cgcaatgcac
    11581 ggatcaccac atcggaacat accccgcgtt cttgcgggac atcaccgcca ggataggtaa
    11641 gctgcacata cgccggatcg taaaatagcg tgctgccgat ttgctgtctg gcaccgtctg
    11701 cgatggcaag gttggtattg gcctggattt gtaccacggt tggtggaatg gcgggagatt
    11761 ttaaggagta gctggtaaat gccgttagca ggctgaccag cgccagtgaa gctttcatct
    11821 gtttcgtccg tgaaaataag agtcacagat tcgaccttcc gggctaacga ttgttctgta
    11881 gagaaaatgt ccagaagcgg ataaaaaatt cctgaacaac gtgggagttt cagattactg
    11941 gcacagagtg ccggatgcgg cgtgaacgtc ttatctggcc taaaaagcac gcaaattcaa
    12001 tatattgcag agattgcgta ggcctgataa gcgtagcgca tcaggcaatt ttgcgttcaa
    12061 actcccggac aagccgggag tttggagtag gttattccca ctcttgcagg aaacgctgac
    12121 cgtactggtc ggctaccagc agagcggcgt aaacctgatc tggcgtcgcg ccgccaggca
    12181 tgttgtggat ggtttcacct tctgcacatg ccgcttctgc cacaattcgc attttcgccg
    12241 ggacatcttc tttaatatcc agttgagcga gagttattgg caaacctacc gcatggctaa
    12301 gcgcagctac ggtttcgatt tcctcaaccg gcgagttttc cagtaccagc tgcgtcagtg
    12361 taccaaatgc cactttttca ccgtgataat agtgatgggc atcaggaatg gcagtcaggc
    12421 cattatgcac cgcgtgcgct gccgccagac caccgctttc aaagccaaca ccgcttaaat
    12481 aagtgttcgc ttcaatcacg cgttccagtg ccggagtcac tacatgctgt tcggcggcga
    12541 gcatcgcttt ttcgccttct tccagcaagg tgttgtagca cagttcagcc agcgccagcg
    12601 ccgcctgagt gcacttgccg cccgccatgg tggtcgcacc gctacgagag caggcacgcg
    12661 cttcaaacca ggtcgccagc gcatcgccga tacccgccgc taacagacgc gcaggtgcgc
    12721 ccgcgacgat tttagtatcg acaatcacca tattcgggtt atttggcagc agcagatagc
    12781 ggtcaaattc accttcgtca gtgtagataa cggaaagagc gctacaaggt gcgtcggttg
    12841 aagcaatggt cggggcaatg gcgaccggaa cacccatgaa atgtgccagt gctttggctg
    12901 tatcgagggt ttttccgcca ccgataccga gaattgcgcc acactgcgca gtctccgcga
    12961 tgccacgcag acggtcgatc tcattttgcg aacattcacc gccaaacggc gcaatctcta
    13021 ctaccagtcc agcatcttta aagcttttct cgacagtgga ttgagcaaaa cctaaaacaa
    13081 atttgtcacc caccactaac cagcgttctg ccagcggctt caggtattcg cccagacgat
    13141 taatcacatc agcgccctgg atgtatttac ccggtgattg aataatgcgg tccataattg
    13201 ctcctttaga gatgagtagt gccaaatgcg gcattccagt cgtgttcgaa cttctctata
    13261 gctgactcta ccgcaggggt gttgagcatt tgttgcgcta catctaaggg cagggtgatg
    13321 gattcacatc ctgccagtaa acagtccagc gcctgacgcg gcgttttaaa gctggccgcc
    13381 agcaccatgc tttctggtgc gtgcatttct aacagcgctt gcagctcctg aaccgtacga
    13441 atgccgtccc cgccctgggc atccacgcgg ttaacatacg gagcaacgta ttttgcccct
    13501 gccagtgcgg ctaataaccc ttgtgcggcg ctatatacag cagtgccaag tgtagtaata
    13561 ccctcttttt tcagtatttt aattgctgcc agaccttcgg aagtcaccgg gattttcacc
    13621 acaatgcccg gaatggcatc gcgcagacgc ttcgcttctt ccaccatccc ctgcgcgtcg
    13681 cggctcatgg tctgagcaaa cagaatgccc tcatcaccaa tcgctttttg cagacgcggc
    13741 agcacttccc atatggactc cttgctggca gcgataatgc tcgggttagt tgtcaccccg
    13801 gcaatcggga atatgtgtgc cagacgttcg acttctgcga cgttagcggt gtccagatac
    13861 agttccatga tgtttccttt tacagttcca gttcatgttg cagcaggctg gcgatagcgt
    13921 cggcagaggc ggcgttaacc agcgcgttac ggaattcttc gtgcatgatg cggcgagcga
    13981 ggcgcgagaa aatgcgcata tgctgatcgc ccgcagcgtg tttgttcagg gttaacataa
    14041 tgatgaattg cgcttcatca tcgccccagc gcaccggcgc ttgcagacgc gctacgctga
    14101 tggtggattg ctcaatgtgt tctgatttgc tgtggggaat ggcaaaacta aagcccagac
    14161 cggtagagaa aacggcctcg cgcgcccaca agtcggcttc cagtttacgc ggatagcggc
    14221 agcggcccgc cagcagcagg ttatcggtca tgcctttgag cacttcttct ttgctgcgcc
    14281 agtcgctttc cagtgtgatg cactcggcgg tgaccagcgg tgcgtcttgt tgggtcatgc
    14341 ggaattgcgc cagcaggtgt tccacttcca gcgaagtacg gcaggccatt gcctggttga
    14401 gcaacttgcg gcactcacgg ctatcaagtt gcgccatccg cgctttcgcc gccggaattg
    14461 atggtgcgct catgctgagt tcatccagcc ctaagccgac cagcaacggc agcacggaac
    14521 ctttcgctcc cagctcaccg cacagaccaa tccatttgcc ctggcgatgc accgcttgca
    14581 cggcgtaatc gagcgcccgc aagaatgccg gattcaggct gttgtagtga cgagtaacct
    14641 tagcgttatc gcgatccact gccagcagat attgcgtcag gtcattacta ccaatactaa
    14701 agaaatcaat ctcttcgcag cattgatcga tgatgaacat caccgacggc acttccagca
    14761 tgatgccgag ctggatcttc tcatcaaacg gaatgtgttc gttacgcagt tgctgtttgg
    14821 cttccgccag tttttctttc acccataaga tctcttccat tgaggagatc atcgggatca
    14881 tgattttcag gctgccgtga gcagaggcac ggaggatcga ccgtagttgt gtggtgaaca
    14941 gcgaggcgta ttcttcataa atacgcaccg cgcgataacc gaggaacggg ttagcttctg
    15001 ccggaatgtt cagataatca acgggtttgt caccgccaat atccatagtg cgcacaataa
    15061 tgctgcgtcc gttggcggac tccagcgctt gacaaaaaat gttgtacaac tcgctttcgc
    15121 ccggtgcgct ggtgcgatcc atatagagca tttcagtgcg gaacaaaccg acgccttccg
    15181 caccattgcc gaatgcggcc tgcgcttcca cggagtgagc gatgttagcg gcaatttcaa
    15241 tgcggatacc gtcagcggta cgggcttgtt gggtcagcca gacacgctgt tgctcacgca
    15301 gggcgtcctg tacgcgggct tcttgctgat aataacgagc tacggcttcc cctggctcaa
    15361 ccacaatcgc cccggcgttg ccgtcgatat aaatcgtttg atgctgccac ggagtaaggg
    15421 catcaatatc tacaccaacc agtgtcggaa tgttgaacga acgggcaaga attacggtat
    15481 gtgaggtggt gccgccactt ttcagtaaca gacctttgag gtgattttta tccagttcga
    15541 ggaactggct gggggttagt tcatcagcca tacaaatggc gggctgcgtc agtttgcccg
    15601 gtgccgggaa gcgttgctca ccgtagattt gctgaagcag ctggaagcag acatcgcgta
    15661 cgtccagggc acgttcttgc aggtagctgc tgctggaacg ggaaaactct tcacaaaagt
    15721 gattcgcact ggcgacaatc gcttcggcgc agcttaatcc ggcgctgacg cctgccagta
    15781 aatgttcgtg cagggaagta tcgccagcca gggatcggtg cgcttccaga atcgcgctgg
    15841 tggcaccgtc gctatccagc agacgaaact caatgttttt cagtaccagc gtcaggccgt
    15901 tttccagtgc ggattgctcg gcgtcaacgt ctttggctgc gggaagatta cccagcgcat
    15961 tgagatctaa agaagagatc ggcgtcagaa tgccgcctgc gctaccgctg cacacggtgc
    16021 gggcgcggat aatttgcgga ttcagattgg tcagtgaaac cggcagtggt tccagttcgt
    16081 cagatttaac ttccgccagc ggcgcatcgc agtgggggaa ttcatcgcgc agccattggc
    16141 ttaaacgctg gtgcgcttcc tgttcgtcgg ccccggaaat cagtaactgg cagttatcgc
    16201 ccgccagcgt gtcggtgcca atcagcgcaa gggcgctttt ggcgttgccc ttgcggtcag
    16261 tgcgcaggtt atgccactca atttgtgatg aaaaagtatt acacagcgtt tcaacgtggc
    16321 ttgccggacg tgcatgtacg ccgttaggta gctcacaaat aaattccaca atcagggcca
    16381 ttgcctctct cccggtacgg ttttctgttt acaggataag agcgcacggc aacggcctgc
    16441 catgtgacaa atctgccaaa agctggacaa atgtaatgta accgtcaatt tgcgacgcgt
    16501 ctcacaagac gccgttttgc ggcatgcttc cggattatcg caagttatga ggcggatcgc
    16561 atttttgtac tgatattaca aaagtccagt aaatggcctt tttatccact gtttgctccg
    16621 cctgcgattg cctattgttt cctccatcaa aaatatggac atgggctttc gcaagcccgg
    16681 gagcagctta tgaatgagtt ggtgcagatc ctgaaaaata cccgtcagca tttaatgacg
    16741 ggcgtttcac acatgattcc cttcgtggta tcgggcggta ttttgctggc ggtttccgtc
    16801 atgttgtatg gcaaaggcgc agtgccggat gccgtagccg atccgaatct gaaaaaactg
    16861 tttgatatcg gcgttgcagg tttgacgctg atggtgcctt tcctcgccgc ttacatcggt
    16921 tactccattg cagagcgttc tgcgctggct ccgtgcgcta tcggtgcctg ggttggtaac
    16981 agctttggtg cgggcttctt tggtgcactg atcgccggga ttatcggcgg catcgtggtg
    17041 cattacctga agaaaattcc ggtgcataaa gttctgcgct cggtgatgcc catcttcatc
    17101 attccgatcg tcggcacact gattaccgca ggcatcatga tgtggggtct gggcgagcct
    17161 gtaggggcgt tgaccaacag cctgactcag tggcttcagg ggatgcagca gggcagcatt
    17221 gttatgctgg cggtgatcat gggtctgatg ctggcgttcg atatgggcgg tccggttaac
    17281 aaagtggcct atgccttcat gctgatttgc gttgctcagg gtgtttatac cgtggtggcc
    17341 attgctgccg tgggtatttg tatcccgccg ctggggatgg ggctggcgac gctgattggt
    17401 cgtaaaaatt tctccgcaga agagcgcgaa accggtaaag cggcgctggt gatggggtgc
    17461 gttggggtta ctgaaggggc gattcctttc gccgcagccg atccgttacg tgtaattcct
    17521 tccatcatgg ttggttcagt ttgtggtgca gtaactgcgg cgctggtcgg tgcgcagtgc
    17581 tatgcaggct ggggtggtct gattgtgctg ccggtggttg aaggtaagtt gggttatatc
    17641 gcagcagtgg ctgtcggggc ggttgtgacg gcagtctgcg tgaacgtgct gaaaagtctg
    17701 gcgcgtaaaa atggatcttc tactgatgaa aaagaagacg acctggattt ggattttgaa
    17761 attaattaat tgaggaacga gccatgacga aaattattgc agtaaccgcg tgtccatctg
    17821 gtgttgccca tacttatatg gctgcagagg cgctggaaag tgccgcgaaa gcgaaaggct
    17881 gggaagtgaa agtagaaacc cagggctcga ttggtctgga aaacgaactg actgcggaag
    17941 acgtggcgag cgccgatatg gtgattctga ctaaagatat cggcatcaag tttgaagagc
    18001 gttttgctgg caagaccatc gtgcgcgtca acatcagcga tgcggtaaaa cgtgctgatg
    18061 ccattatgag caaaattgaa gctcacctgg cgcaaaccgc ttaagttctt ctcccgctcg
    18121 caagggcggg ttcgctttcc cacaggagtt cctcatgacg aatcgtatct ctcgcctcaa
    18181 aactgcactg tttgccaata cccgtgaaat ctcgctggag cgtgcgctgc tttataccgc
    18241 cagccatcgg caaaccgaag gcgaaccggt gatattgcgc cgggcgaaag caacagcgta
    18301 tatccttgaa catgttgaaa tttcgattcg tgatgaagaa ctgattgccg gtaaccgcac
    18361 cgtaaaaccg cgcgccggga ttatgtcgcc ggaaatggac ccttactggc tgctgaaaga
    18421 gctgggtcaa ttcccgacgc gtccgcagga ccgttttgct atcagcgaag aagataaacg
    18481 tatctaccgc gaagagttgt tcccgtactg ggaaaaacgt tcgatgaaag atttcatcaa
    18541 cgggcagatg acggatgaag taaaagccgc gactagcacg cagattttca gcatcaacca
    18601 gacggataaa ggccaggggc acattattat tgattacccg cgcctgctga atcacgggct
    18661 gggggagctg gtggcacaga tgcagcaaca ttgtcagcaa cagccggaga atcactttta
    18721 tcaggcagcg ctgttactgc tcgaagcctc gcagaaacac attttgcgtt acgccgaact
    18781 ggcggaaacg atggcggcaa gctgcactga tgcccagcgt cgcgaagaac tgttgactat
    18841 tgcggagatc tcccgccata acgccgaaca taaaccgcag acgttctggc aggcgtgcca
    18901 gttattctgg tacatgaaca tcattctgca atacgaatcc aacgccagtt cgctgtcttt
    18961 agggcgcttc gaccagtata tgttgccgtt ctatcaggca tctttaaccc agggcgaaga
    19021 tccggcgttc ctgaaagaac tgctcgaatc tttatgggtg aaatgcaacg acattgtgct
    19081 gttgcgctcc accagcagcg cgcgttattt cgcaggtttc ccgactggct ataccgcact
    19141 gctcggcggg ttaaccgaga acggacgtag cgcggtgaac gtgctttcgt tcctctgcct
    19201 cgatgcctat caaagcgtac aattaccgca accgaaccta ggggtgcgca ctaacgcctt
    19261 gatcgacacg ccgttcctga tgaaaaccgc agaaaccatt cgcctcggca ccggtattcc
    19321 gcaaatcttt aacgatgaag tggtggtgcc agcgttcctc aaccgtggcg tttcgctgga
    19381 agatgcgcgc gactattccg tagtgggctg tgtggaatta tctattcccg gcagaaccta
    19441 cggcttgcat gacatcgcga tgtttaatct gctgaaagtg atggaaatct gcctgcatga
    19501 aaatgaaggc aatgctgcgc tgacttatga aggtttactg gagcagatcc gcgccaagat
    19561 cagccactac atcaccctga tggttgaggg cagcaatatt tgtgatatcg gccatcgcga
    19621 ctgggcacct gtaccgctgc tctcgtcttt tatcagcgat tgtctggaaa aaggtcgcga
    19681 tattaccgat ggcggcgcgc gttataactt ctccggcgta cagggaattg gtatcgccaa
    19741 cctgagcgat tctctccatg cgttgaaagg gatggttttt gatcaacagc gtttaagttt
    19801 tgacgaattg ctgtcggtat taaaagccaa ctttgcaacg ccagaaggcg aaaaagtccg
    19861 cgctcgctta attaaccgct ttgagaaata cggtaacgat atcgacgagg tgggtaacat
    19921 tagcgccgaa ctgttgcgcc actactgcaa agaagtggaa aaataccaga acccgcgcgg
    19981 cggttacttc acgccgggat cgtataccgt ttctgctcac gtcccgttgg gatcggtggt
    20041 tggcgcgacg ccagacggtc gttttgccgg agaacagttg gcagacggcg gcttgtcacc
    20101 catgctgggc caggacgcac aagggccaac agcggtactg aagtcagtca gtaagctcga
    20161 taacacgctg ctgtctaacg gtacgttgct gaacgtgaaa ttcactccgg cgaccctgga
    20221 aggtgaagcg ggattacgca aactggccga cttcttacgg gcgtttaccc agcttaagtt
    20281 gcagcatatt cagtttaacg tggtgaacgc cgacacgttg cgggaagcgc aacagcgccc
    20341 acaagattat gccgggctgg tggtgcgcgt tgccggatac agcgccttct ttgtcgaact
    20401 gtcgaaggag atccaggatg acatcatccg ccggacagcg catcagctgt aacgttgtgg
    20461 aaacgcgccg caatgatgtg gcgcgcattt tcaacattca gcgttattca ctgaatgacg
    20521 gcgagggcat tcgtacggtg gtctttttta aaggctgtcc gcatctttgc ccgtggtgtg
    20581 ctaatccgga gtcgatctcc ggcaaaatcc agacggtacg cagagaggcg aaatgtctgc
    20641 actgtgcgaa atgtttgcgt gatgcggatg aatgcccctc cggggcgttt gaacggattg
    20701 gtcgcgatat cagccttgac gcgctggaac gggaagtgat gaaagatgac attttctttc
    20761 gcacgtccgg cggcggcgtc acgctttctg gcggcgaagt gttaatgcag gcggagtttg
    20821 ctacccgttt tttacagcgg ctgcggttgt ggggcgtgtc atgcgccatt gaaactgccg
    20881 gagacgcgcc agccagcaag ctgttaccgc tggcgaaatt gtgcgatgaa gtgttgttcg
    20941 atttaaaaat tatggacgcg actcaggcgc gggatgtggt gaagatgaac ctgccacgcg
    21001 tgctggagaa tctgcgtttg ctggtgagtg agggcgtcaa cgtgatcccg cgtttaccgc
    21061 tgatccctgg tttcacgctc agccgggaga atatgcagca ggcgctggat gtactgatcc
    21121 cgctgaaaat caggcagatc catctgttac cgtttcatca gtacggcgaa ccgaaatacc
    21181 gcctgctggg gaaaacatgg tcgatgaaag aggtgcctgc gccgtcgtca gccgatgtga
    21241 caacgatgcg cgaaatggca gaacgggccg gatttcaggt taccgtggga ggttaaaatg
    21301 gcatacctgg tggcagtaac cgcctgcgta agcggcgtgg cgcatacata tatggcggcg
    21361 gaacggctgg aaaagttgtg cctgttagag aagtggggag tcagcattga aactcagggc
    21421 gcgctgggaa cggagaatcg tttagcggac gaggatatcc gtcgggcgga tgttgctctg
    21481 ttgattacgg atatcgagct tgccggtgcc gagcgatttg aacattgccg ctatgtgcaa
    21541 tgcagcatct acgcattcct gcgtgagccg cagcaggtaa tgagcgcggt gcgcaaagtg
    21601 ctttctgcgc cgcagcaaac ccatcttatt ctggagtagt cggtttttct gtcagttggc
    21661 tgtggtactg ccggcgatat tccgacggcg agcgttcggt gtttttacga aacagacggc
    21721 agaagtagtt gctgtcgaca aagccgcagg cgtgcgccac ttcttttacc ttcaggtcat
    21781 agcctttcaa taacgtctta gcgtgctcca gtcgcgtgtg attcaggtat tcgttaaaac
    21841 caatggcccc cgttttttga aacaggtgcg agagatagtt tggtgaaata tagaatgcct
    21901 gtgcgacaga ttcgcgggta agcgcggagg catagcgttc gtcgatataa tcgcgaatag
    21961 cttcgaatag tgcctggctg cgtgaggcgg tctggatttg gctgccaagc aaatcgcggc
    22021 agtggctaag caggctggcg acgataagcc ttgccgtttg ctgctcctgc ggctgcattt
    22081 gcatttcatt gagcgtttgt agcagaaaag aacctatgcg tgggccgcgt cgggcgacgt
    22141 gttgcttcgc cagattttga tattgtttgc catcccattg cacgacgctg aagccgagtt
    22201 gctgtttgcc aaacagcacg ctaaaggtag tagcaggggc ttgccattgt ggaaaattcc
    22261 agccaccagc ggcaacgtac agcacatcgc cgggaactaa cgttgctcca gcgccggtat
    22321 caacaaactc accttccagc acaatttcca gccgtggaaa atcgacctga tacgcaagat
    22381 cgggcaccgg gccgttactg ctggcaaagt aaatctggcg cagggacagc gggccgttga
    22441 tcaggcggga gagcagatag ctgacgtcgt gatacatgtt attcccttag gtatggagac
    22501 gggaaaacag taactggcat tattaaaaaa ggccacctga aaagtgtggc ctgaggggag
    22561 ttcgatgtaa acattgcggc agttgtattg ccggatgtgg cgtaaacgcc ttatccagtc
    22621 taccgccgcg atctgtaggc cggataagac gcgtcaagcg tcgcatccgg caaatagcgc
    22681 ctggctgata attactgatt acctacctgg tcgccatacg gcagcgtgtc gtaatcgatc
    22741 agcgcgtttt tcttgtacgg gttaccaatc cagcgggtat tttctttgaa ctgcggattc
    22801 accactgaac gggttggatc gtaaccgtcg taagataagc ccgctaaatc tgaccaggtg
    22861 tggatcagtt ccgcaaggct gtatttacga tcaacatcct gcgagaaatc acgcggatga
    22921 gtcgcttgcc atttttccga cgtccacagc aggaacggaa tggtgtacat atgacgcgtc
    22981 gggttgtcct cattacgccc ctgggtttta tgaggcggcg tgtcgtaaac ctcttcaccg
    23041 tggtcagaga agtaaaccag gaaaccgttc gggtctgctg ctttaaagtc tttaatcagg
    23101 ctggcaacca catgatcgtt atacaggtta gcgttgtcat aatcgttata tgactccagc
    23161 tcttccgcgt ttaatcccgg cggaacatga tcggtattgc catcaaactt gccctgattt
    23221 tccgggtagc ggtatttgta tttgatatgc gtacccagca gatgaacgat gatcagtttc
    23281 ttcggcgcag ggtccttcag cacttcctgg aacggcttca gcacgttagt gtcgtattca
    23341 cgcgcactct gcgtacgttg ctggttcatg tagtactgct tgtcggtctg gcgcgaaaat
    23401 accgtcagca tggtattgcg ggcggtcatc gtctgctggt tggtgatcca gaaggtttta
    23461 taacccgcct gtttcatcat gttcatcagc gacggctgcg tcagatacag atccgggttc
    23521 ttttcattgg caaaggtcag cgcctgttgc aggatttcaa tggtgtacgg acgagacgta
    23581 actacgttat taaacacggt cagattcgga tcggttttat gcagcgcatt cagctccggc
    23641 gtggtttcac gcggataacc gtacagactc atgcgtccac gctgggttga ctcgccaatc
    23701 accagcacta aggtgcgcgg ttcgttaccc gattcatctt tgaaattagc cagcggcggc
    23761 aaggcattat tttcattcag tagctttgtt agcgagttta gttgctgacg atactgataa
    23821 tagccggtca ggaattgcca cggtgcggca ggctccatac gcgaggccag gttatccaac
    23881 gttttctcaa acggcttgtt tttgataaac atattcatgg cgatcggatg cagaatcaag
    23941 ccataaagca gggcaaaaga gacaacataa cgccacggct ttggaatata gaccgggcgc
    24001 aggcgtgtcc acagcagaac tgccaccgcc gtataggcca gcgcgataag cacaattttc
    24061 aggctgaaat actggcttaa atactcgctg gcttcgttgg tgttggtttc gaacatcaca
    24121 aacagaacgc tctgcgagaa ctcctgaccg tagatgacgt agtagcacag cgccgccaga
    24181 gaggccgccc atagcaccac gccgatcact gcggcaataa ttttgatccg cttcggaaag
    24241 aggaataccg gaattaacca cagcgagctg aataacagcg agtcgcgaat gccgttggtg
    24301 ccactataac cactgatgta aataatggcc tgtagcagag tagagaaaaa ccaaaagtag
    24361 agcaatgccc aacccagggc tttccagcta aaaagaggtt tagcctggac ttctgtggaa
    24421 tgcatagtaa gaacctgtct tgaaaaaaat atcgccgaat ttaccgacaa ttccttaagg
    24481 atatctgaag ggatatccag aatttgaata aaatgcagac agaaatatat tgaaaacgag
    24541 ggtgttagaa cagaagtatt tcagaaaacc ctcgcgcaaa agcacgaggg tttgcaggag
    24601 aggaagatta gccggtatta cgcatacctg ccgcaatccc ggcaatagtg accattaacg
    24661 cctgctcgac gcgaggatcc ggttcctggc cttctttttc tgcctggcgg gagcggtgca
    24721 gcaactcggc ttgcaatacg ttcagcgggt cggtgtaaat attccgtagc tgaatagact
    24781 ctgcaatcca cggcagatcg gccatcagat gggaatcgtt ggcaatcgcc agcaccactt
    24841 tgatgtcttc ttcttgcaga ttgcgtaact ctttacctaa cggccacagt gttttgtcta
    24901 ccaggcgttg gtcatagtat tccgccagcc acaggtctgc tttggcgaag accatctcca
    24961 gcatgccgag acgcgtcgag aagaatggcc aatcgcggca catagcctcc agctcgctct
    25021 gtttgccatc ttcgaccact ttttgcagcg ccgtacctgc acccagccag gcggggagca
    25081 tcagacggtt ttgcgtccag gcgaagatcc acggaatggc gcgtagtgac tcgacgccgc
    25141 cggttgggcg acgtttcgcc ggacgtgaac ccaacggcag tttgcccagt tcctgttccg
    25201 gcgtagcgga gcggaagtaa ggcacaaaat ctttgttttc acgtacgtag ccacggtaga
    25261 gatcgcagga gatgactgat agttcatcca taatgcgacg ccagctctct ttcggctcag
    25321 gtggtggcag caggttggct tccagtatcg ccccggtgta aagcgacagg ctgctgacgg
    25381 tgatttctgg cagaccatat ttaaagcgga tcatctcgcc ctgttcagtc acgcgcaggc
    25441 cacctttcag gcttcctggc ggttgtgaca gcagcgccgc atgagcaggt gcgccgccgc
    25501 gaccaatgga accgccgcga ccgtggaaca acgtcagctc aatacccgct ttttcgcagg
    25561 ttttgattaa tgcatcctgt gcctgatatt gcgcccagga cgctgccatc acgcccgcat
    25621 cttttgctga gtcggaatag ccaatcatca ccatctgttt gccctgaatc aggccgcgat
    25681 accagtcgat attcagcaac tgagtcatga catcgttggc gttgttcagg tcatcgagag
    25741 tttcaaacag cggagcaacc ggcatcgcaa acccgatacc cgcttctttc agcagcaggt
    25801 ggacagccag cacgtcggac ggcgttttcg ccatcgagat cacgtaggcg gcaatggagc
    25861 cttgcggtgc ttcggcaatc acctggcagg tatcgagcac ttcgcgcgtt tcggcgcttg
    25921 gttgccagtt gcgcggcaga agcggacgtt tggagttcag ttcgcggatc aggaacgcct
    25981 gtttgtcggc ctctgaccag ctttcgtagt cgccgatacc gaggtagcgg gtcagctcgc
    26041 ccagcgcttc ggtatgacgc gtgctctcct gacggatatc aatacggacc agcggtacgc
    26101 cgaaacattt cacgcggcgc agggtgtcga gcagatcgcc gttggcgata atacccatgc
    26161 cacacgcctg aagtgactgg tagcaagcgt agagcggttc ccacagttct tcgttttgtg
    26221 tcagcaggcc ttctggtttt ggcagttctt cgcctttcag gcgcgcttcc agccatgcct
    26281 gtgtcgccat caggcgagaa cgcaggtttt tcatcagata gcgatacggt tctgcggcac
    26341 cttcttcgcc aaccagcgcc agcagttcag gggtcgcttc aaccatcgac agttcagaaa
    26401 ccagaacctg aatatctttc aggaacagat cggtggcttt ccagcggctg agcagcagga
    26461 cgtggcgggt gatatcggca gtgacgttcg ggttgccgtc gcggtcaccg cccatccacg
    26521 aggtaaaacg taccggaaca aattcgacgg gcagtttgta gccgaggttc tcttccagtt
    26581 gttcgttcag ttcgcgcagg taattcggta cgccttgcca caggctgttt tctactacgg
    26641 caaagcccca tttggcttca tctaccgggc ttggacgcag cttacggatt tcatcggtat
    26701 gccatgactg ggcgatcaac tggcgcaggc gacgcatcag ctggttgtgt tcgtagtcag
    26761 cgatatcttt gttatcgagc tgttttaagc aggcgttcac ttccaccatt ttgtggatca
    26821 gtgtacgacg ggtaatttcg gttgggtgag ccgtgaggac cagttccagc gacagcgatt
    26881 ccactgcttt tttgatggtg tcttcgctca gttccggctg gtttttcagt ttacgcaggg
    26941 tgcgggcgat cacttccggg ttgctggcag cttcgccttt cggcgaaatg ctgtggtatt
    27001 gctcggcggt gttggccagg ttcaggaact gactaaacgc acgcgcaacg ggcagcagct
    27061 cgtcgttcga caaattttgt aaggtggtga gtaactcctg gcggttagca tcattgccag
    27121 cgcgtgaaga tttggacaac ttacggatag tttctacgcg ttcaagaatg tgttctccca
    27181 acgcatcctt gatggtttct cccagcactt tgccgagcat actgacatta ctacgcaatg
    27241 cggaatattg ttcgttcata ttaccccaga caccccatct tatcgtttga tagccctgta
    27301 tccttcacgt cgcattggcg cgaatatgct cgggctttgc ttttcgtcgt cttttataaa
    27361 gccacgtaaa agcggtgacg tcaaatgctg cgaaatcgct tcagcaaacg aataaatagc
    27421 aggaatttac gtcattaaat tcacgacgct ttaaataagc gtaacttatg ggaatgttaa
    27481 aaaatcgcct caagtaacac caaaggtgta ggtcggataa gatgtgcaag catcgcatcc
    27541 gacattattg cggcactgga gtttggcaac agtgccggat gcggcgcaag cgccttattc
    27601 ggcctacgtt ttaatgccag caaaaatggt gaatgacctg ggttatcagt tcgcgggtgg
    27661 gcttgataaa ccgtgtttcc agatattcat caggctgatg agcctgatta attgagccag
    27721 gccccagaac cagcgtcggg cataacgttt gaataaacgg cgcttcggta cagtaattca
    27781 ccacttcggt ttttgctccg agcaatttct caaccacttc aaccagttga tgattcggtg
    27841 ggcattcata gccagggatc ggcggatgca gctcgtcgac cgtcagacga cccggccagc
    27901 gttcgctcac cggagccaat gcatcgttga gcaaaccatt aagttcattg agtgtcatgc
    27961 caggcagcgg acgaatatcc atatgcaact cacaccaagc gcaaatacgg ttagaagcgt
    28021 cgccaccgtg aatatgcccg aggttgagcg tagggtatgg cacggtaaac gcttcgtagt
    28081 gataacgttc tttcaggtta tcgcgcaatt gcaaaatatg cccgatggcg tcgtgcatta
    28141 gttctatagc gttaactccg cgtgctggat cgctggagtg ccccgactgg ccctgaatac
    28201 ggatggcgtt agagatatga cctttatgtg cgcgtaccgg ttgtagtgac gtcggttcgc
    28261 caatgatggc gcaatccggg cgcagggcgg tagtttcggc aaaataacgc gctccggcca
    28321 tactggtttc ttcatcagca gtcgccagaa tgtagagcgg ttttttcagt ttcgtgacgt
    28381 cgacatcgcg tagcgcatca aggataaacg caaaaaagcc tttcatgtcg gcggtgccta
    28441 agccgtaaag cttgccgtca tgctccgtca gtgtaaacgg atcgcgcgtc cagcgaccgt
    28501 catcaaatgg caccgtatcg gtatgccccg ccagcaacaa gccgccagcc ccctgtccgg
    28561 tacttgccag catattaaat ttgttgcgag ttcctggtac aggctgcact tccacattga
    28621 agcctaaatc tttaaaccag tccgccagca gagtgattaa atctgcattg ctttgatcga
    28681 gtgcctcttc cgtggcgctt attgaaggtg tggcaatcag agcacggtaa atctcgataa
    28741 atggcggtaa tttgtttttc attgttgaca cacctctggt catgatagta tcaatattca
    28801 tgcagtattt atgaataaaa atacactaac gttgagcgta ataaaaccca ccagccgtaa
    28861 ggtgaatgtt ttacgtttaa cctggcaacc agacataaga aggtgaatag ccccgatgtt
    28921 gaatacgctg attgtgggtg ccagcggcta cgctggcgca gagctagtga cctgtgtaaa
    28981 tcgccatccg catatgaaca taaccgcttt gactgtttca gcgcaaagca atgatgcggg
    29041 aaagttaatc tccgatttgc atccgcagct aaaaggcatc gttgatctgc cgttgcagcc
    29101 gatgtcggat atcagcgagt ttagccctgg ggtggacgtg gtgtttctcg ccaccgccca
    29161 tgaagttagc cacgatttag cgccgcagtt tcttgacgcg ggctgcgtgg tgttcgacct
    29221 ttccggcgcg tttcgtgtta acgacgccac cttctatgaa aaatattacg gctttaccca
    29281 tcaatacccg gaactgctgg aacaggcagc ctacggtctg gcggagtggt gcggtaataa
    29341 attaaaagaa gcgaatttga ttgcggtgcc aggctgttat ccgacggcag cacagttggc
    29401 gctgaaaccg ttgattgatg ccgatcttct tgacctcaat cagtggccgg tgatcaacgc
    29461 caccagcggc gtgagcggtg caggacgtaa ggcagcgatt tcaaatagtt tttgtgaagt
    29521 tagcctgcaa ccgtatggcg tctttaccca tcgccatcaa ccagagatcg ccacacacct
    29581 cggtgctgac gttatcttca ccccgcatct gggcaatttc ccgcgcggca ttctcgaaac
    29641 cattacctgc cgcctgaaac cgggtgtgac ccaggcgcaa gtggcgcaag cgttacagca
    29701 ggcgtatgcc cataaaccgc tggtgcggct gtatgacaaa ggcgttccgg cgctgaaaaa
    29761 tgtcgttggg ctaccatttt gcgatatcgg gtttgccgtt cagggcgaac atctgattat
    29821 tgtggcgacc gaagacaact tactgaaagg cgcggcggca caagcggtac agtgcgccaa
    29881 tattcgtttc ggctatgcgg aaacgcagtc tcttatttaa gggtgcaatg atgaatccat
    29941 taattatcaa actgggtggc gtactgctgg atagtgaaga ggcgctggaa cgtctgttta
    30001 gcgcactggt gaattatcgt gagtcacatc agcgtccgct ggtgattgtg cacggcggcg
    30061 gttgcgtggt ggatgagctg atgaaagggc tgaatctgcc ggtgaaaaag aaaaatggcc
    30121 tgcgggtgac gcctgctgat cagattgaca ttatcaccgg agcactggcg ggaacggcaa
    30181 ataaaaccct gttggcatgg gcgaagaaac atcagattgc ggccgtaggt ttgtttctcg
    30241 gtgacggcga cagcgtcaaa gtgacccagc ttgatgaaga gttaggtcat gttggactgg
    30301 cgcagccagg ttcgcctaag cttatcaact ccttgctgga gaacggttat ctgccggtgg
    30361 tcagctccat tggcgtaaca gacgaagggc aactgatgaa cgtcaatgcc gaccaggcgg
    30421 caacggcgct ggcggcaacg ctgggcgcgg atctgatttt gctctccgac gtcagtggca
    30481 ttctcgacgg caaagggcaa cgcattgccg aaatgaccgc cgcgaaagca gaacaactga
    30541 ttgagcaggg cattattact gacggcatga tagtgaaagt gaacgcggcg ctggatgctg
    30601 cgcgcacgtt gggccgtccg gtagatatcg cctcctggcg tcatgcggag cagcttccgg
    30661 cactgtttaa cggtatgccg atgggtacgc ggattttagc ttaagttttg ttggccggag
    30721 gcgcagcttt ccggcattga atttcaaaat aaggaaacag agttatggca ctttggggcg
    30781 ggcgttttac ccaggcagca gatcaacggt tcaaacaatt caacgactca ctgcgctttg
    30841 attaccgtct ggcggagcag gatattgttg gctctgtggc ctggtccaaa gccctggtca
    30901 cggtcggcgt gttaaccgca gaagagcagg cgcaactgga agaggcgctg aacgtgctgc
    30961 tggaagatgt tcgcgccagg ccacaacaaa tccttgaaag cgacgccgaa gatatccata
    31021 gctgggtgga aggcaaactg atcgacaaag tgggccagtt aggcaaaaag ctgcataccg
    31081 ggcgtagtcg taatgatcag gtagcgactg acctgaaact gtggtgcaaa gataccgtta
    31141 gtgagttact gacggctaac cggcagctgc aatcggcgct ggtggaaacc gcacaaaaca
    31201 atcaggacgc ggtcatgcca ggttacactc acctgcaacg cgcccagccg gtgacgttcg
    31261 cgcactggtg cctggcctat gttgagatgc tggcgcgtga tgaaagccgt ttgcaggatg
    31321 cgcttaagcg tctggatgtc agcccgttag gttgtggcgc gctggcggga acggcttatg
    31381 aaatcgaccg tgaacagtta gcgggctggc tgggatttgc ttcagcaacc cgtaacagtc
    31441 tcgacagcgt ttctgaccgt gaccacgtgc tggaactgct ttctgctgcc gctatcggca
    31501 tggtgcatct gtcgcgtttt gctgaagatc tgattttctt taacaccggc gaagcggggt
    31561 ttgtggagct ttctgaccgc gtgacttccg gttcatcatt aatgccgcag aagaaaaacc
    31621 cggatgcgct ggagctgatt cgcggtaaat gcggtcgggt gcagggcgcg ttaaccggca
    31681 tgatgatgac gctgaaaggt ttgccgctgg cttacaacaa agatatgcag gaagacaaag
    31741 aaggtctgtt cgacgcgctc gatacctggt tggactgcct gcatatggcg gcgctggtgc
    31801 tggacggcat tcaggtgaaa cgcccgcgtt gccaggaagc ggcgcaacag ggttacgcga
    31861 acgccactga actggcggat tatctggtgg cgaaaggtgt gccgttccgc gaggcacacc
    31921 atattgtggg tgaagctgtg gtggaagcca ttcgtcaggg caaaccgctg gaggagctgc
    31981 cgctcaccga gttgcagaaa tttagtccgg tgattggtga agatgtctat ccgattctgt
    32041 cgctgcaatc gtgcctcgac aagcgtgcgg caaaaggcgg cgtctcacca cagcaggtgg
    32101 cgcaggcgat tgcttttgca agggcgcggt tggggtaaga acatttatat gtacaaattt
    32161 gagcctggct tatcgccggg cttttttatg gcaaaaaaaa gcggatcctg gagatccgca
    32221 aaagttcacg ttggctttag ttattcgagt tgagaaactc tcgaaacggg cagtgacttc
    32281 aagggttaaa agaggtgccg ctccgtttct gtgagcaatt atcagtcaga atgcttgata
    32341 gggataatcg ttcattgcta ttctacctat cgccatgaac tatcgtggcg atggaggatg
    32401 gataatgaat attcgtgatc ttgagtacct ggtggcattg gctgaacacc gccattttcg
    32461 gcgtgcggca gattcctgcc acgttagcca gccgacgctt agcgggcaaa ttcgtaagct
    32521 ggaagatgag ctgggcgtga tgttgctgga gcggaccagc cgtaaagtgt tgttcaccca
    32581 ggcgggaatg ctgctggtgg atcaggcgcg taccgtgctg cgtgaggtga aagtccttaa
    32641 agagatggca agccagcagg gcgagacgat gtccggaccg ctgcatattg gtttgattcc
    32701 cacggttgga ccgtacctgc taccgcatat tatcccgatg ctgcaccaga cctttccaaa
    32761 gctggaaatg tatctgcatg aagcacagac ccaccagtta ctggcgcaac tggacagcgg
    32821 caaactcgat tgcgtgatcc tcgcgctggt gaaagagagc gaagcattca ttgaagtgcc
    32881 gttgtttgat gagccaatgt tgctggctat ctatgaagac cacccgtggg cgaaccgcga
    32941 atgcgtaccg atggccgatc tggcagggga aaaactgctg atgctggaag atggtcactg
    33001 tttgcgcgat caggcaatgg gtttttgctt tgaagccggg gcggatgaag atacacactt
    33061 ccgcgcgacc agcctggaga cgctgcgcaa catggtggcg gcaggtagcg ggatcacttt
    33121 actgccagcg ctggctgtgc cgccggagcg caaacgcgat ggggttgttt atctgccgtg
    33181 cattaagccg gaaccacgcc gcactattgg cctggtttat cgtcctggct caccgctgcg
    33241 cagccgctat gagcagctgg cagaggccat ccgcgcaaga atggatggcc atttcgataa
    33301 agtgttaaaa caggcggttt aaaccgttta acgcagctac ccgataggct tccgccatcg
    33361 tcgggtagtt aaaggtggtg ttgacgaagt actcaatagt gttgccgcca cctttctgtt
    33421 ccataatcgc ctgaccgata tgaataattt cggcagcgcg ctcgccaaag cagtgaatgc
    33481 ccagaatctc ttttgtttcc cgatggaaca aaattttcag cgtgcccacg ttcatgccga
    33541 cgatttgtgc acgtgccaga tgtttgaact gggcgcggcc cacttcatat ggcactttca
    33601 tcgcggtcag ctgctgttcg gttttgccca cagagctgat ttccgggatg gtgtaaatgc
    33661 cggtagggat atcttcaatc agatgcgcgg tggcttcgcc ttttaccagc gcctgcgcgg
    33721 caatgcgccc ctggtcatag gccgctgatg ccaggctcgg ataaccaatc acgtcgccca
    33781 ccgcgtaaac gtgcggctgt gcggtctgat acatgctgtt gaccttcagc tgtccgcgac
    33841 tgtcagtttc cagcccaatg ttctgtaacg ccagcgaatc ggtattaccg gtacgaccgt
    33901 tggcatagag caggcagtca gctttcagtt ttttacccga cttcagatgc atgatcacac
    33961 cgtcgtcaca gccttcgatc ttctcgtact cttcgttgtg acgaatcact acgccactgt
    34021 tccagaagtg ataggagaga gaatctgaca tctcttgatc gagaaatgcc agcaggcgat
    34081 cgcgggtgtt gatcagatcc acttttacat ccataccgcg gaagatcgac gcatattcac
    34141 agccgatcac tccagcacca tagataagta catggcgcgg ttcgtggtgc atgctgagaa
    34201 ttgagtcgct gtcgtaaatg cgtggatggg tgaaatcaac atctgttgga tgatatggac
    34261 gagagccgca ggcaataaca aatttttcag cggttagtgt ttcaacgctg ccgtccgggc
    34321 aatccagcgc caacgtatgc tcgtcaacaa agcgagcgtt cccctgcaat atttcacagt
    34381 gattacgttc gtaaaatccc tgacgcatgc gcgtttgttg attaatcacg ttatcggcat
    34441 ggttaaggat atcggcaaaa gaagagcgga gcagtcggga atggtcgctg tatagtgggt
    34501 tttgattgaa ttctataatg cggctgacgg cgtgacggag agctttcgac gggatggtgc
    34561 cccagtgggt gcaaccgccg ccaacatttt gataacgctc gataactgcg acgcgcgcac
    34621 cttgcttaac caggcccatt gcagcgcctt cgccgccggg gccggaacct attactatgg
    34681 catcgtaatc gtaggaatgt ggcatggtag ggcttacctg ttcttataca taaaagcaac
    34741 agaatggtaa cattttatcg cgggtaagcc aattgatccc cgtcattaat ctggctatag
    34801 cgtgagcggc ctttgctttg tctgcctcct tcttaaccca tttattcact attaatcgct
    34861 ttttattatg gctgatagtt ttatcttgcc gaagcgtgct tttttgttat tgtctttgtg
    34921 aaatcttctt ataaaacaaa catataaata ttttatattg atgagttatt tgtatttaca
    34981 ccatcaataa aatccgttaa ttagcctgtt tataagtgaa taaaggaata gccgatgatc
    35041 gaacactata ttcgagggtt tgaggaagaa ctgcgtgaga tccgccatca gatccacgaa
    35101 aacccggagc tgggattaca agaatttaaa accagtgctt tggttgcgga aaagctgcgt
    35161 cagtggggct atgaagtaga gcaaggactg gcgacgacgg ggatagtggc cacgttaaaa
    35221 gtgggcgacg gagagaaaag tattggcctg cgggcggata tggatgcgct gccgatttat
    35281 gaaaatagtg gcaagccctg ggccagtaaa catcctggtt taatgcacgc ctgcgggcat
    35341 gacgggcata ccaccatact gctgggggcc gcgcgttatt ttgccgaaac ccgccgtttt
    35401 aacggcacgt tacgtcttat cttccagcct gcggaagaga tgattaatgg cggtgagatc
    35461 atggttaaag aggggctttt tgatcatttc ccctgcgatg tcattttcgg catgcataac
    35521 atgcccggtc tgccggtggg taagttttat ttccagccag gggcactgat ggcgtcaatg
    35581 gatcagttcc atattacagt tcgtggttgt ggcggacacg gtgcgatccc gcacaaagcc
    35641 atcgatccgg tgctagttgc cgcacatatc accaccgcat tacaaagcat tgtgtcacgc
    35701 aatgtcgatc cgctggaagc ggcggtaatt accgtcggca gcattgttgc gggtgaggcg
    35761 gctaacgtca ttcctgacag tgctgagatg aaaatcagcg tccgctctct tagccgcgat
    35821 acccgacaac ttcttctgac ccgtattcct gctctggcac aagcccaggc tgccagcttt
    35881 ggcgctacgg ctgaggttac gcatgttaac ggtacaccag tattagtaaa tgacgaggag
    35941 atggcgcgct tcgcctggca ggtggcgtgt aaaacgtttg gtgaagatcg ggccgagttt
    36001 ggcatcaagc ccctgatggg cagtgaagat ttctctttca tgctggaagc ccaacccaaa
    36061 ggcggtttcc tgttattcgg taatggcgat gtcggggaag gttcctgcat ggtgcataac
    36121 ccgggttacg acttcaacga tgcaagtctg gtcccggcaa gcagctactg gggcgcactg
    36181 gtagaagcct ggctgcaata aataaaacaa cacacaacaa cactttttgg caaggaacat
    36241 ggaatgacgg cgatcgtaaa tgaaaccgtg agtgtgaaga aaaagcccag cggcaggcgg
    36301 gtgatttttg cctcggcgtt tggcaatgcg ctcgaatttt ttgattttgg cgtttacaac
    36361 ttttttgtgg tctatatcag cacgctattt tttccgccca gtgcagataa aaacgtcgcg
    36421 ctactgctgg cgtttgccac tttcggcgtg agctttttta tgcgcccgct cggcggcatt
    36481 attgtggggg cctgggccga tcgtttcgga cgcaaacccg cgatggtgtt caccattgcg
    36541 ctgatgagtc ttggtacact gatgatcggc atcgcaccga cgtatgaaac ggcgggctat
    36601 tggggaaccg cgacgctggt gctagcgcgt ctaattcagg gcgttgcggc aggaggcgaa
    36661 gtcggtgcct caatgtcttt gttggttgaa tcagctccgg caaatcgtcg cggtttttac
    36721 agcagttggt cactggcgac tcaaggcctg gcaacaactt ttggaggtgt tgtcgcttta
    36781 ggtttaagtg catggctgcc ctttgcgacc ggttcagaaa ccgtcatggc tgaatggggc
    36841 tggcgtgtgc cgttttttat tggcgtgtta ttagcgcctg tcggctgttg gttgcgtttg
    36901 agtctggaaa atgatgttcc tgagccagca cataataaga aggccgcagc cagcgaaagc
    36961 gccttctcct tacttatgca acataaagcg actatcgcta acggtatctt gctcgctatt
    37021 ggtagtacag tcgcaaccta tatctccctc ttttattacg gtacatgggc ggcgaagtat
    37081 ttaggaatga accaaaacta ttctcatgca gcgatgttac tggcgggcgt tattacgttt
    37141 gtgggtgcgc tgctggtggg gatgttgtgc gattcggtcg ggcgtaaaaa gctgatttta
    37201 atctcccgtg tgatggtgct gatctgtagc tggccgtcat tctggctgtt ggtgaattac
    37261 ccaagcccag gcatgttgct gacggtggtt ttcgtgatgg tcagctttac cacgcttggc
    37321 ggtgtgccag tgatgttgtt gatctctgaa ctgttgccga aacggattcg ggcattgggt
    37381 tttgcgctgg tttatagcat tggtgtggca atctttggcg gttttgctca atatttcgct
    37441 acgcaatcca tcgtgttgct ggatagtttg acggctccgg cgtggtatct gggtggaggc
    37501 acgttgctgt cgatgttggc gttgttgtac gtaaaagaac cggccaaaga attgcagtaa
    37561 atatgtttta ttgcgttacc gttcattcac aatactggag caatccagta tgttcattct
    37621 ctggtatagt gccagcagta cttttggcaa ggattcagac atcgtgatgg gcgtaagagc
    37681 gcaacaaaaa gaaaaaaccc gccgttcgct ggtggaagcc gcatttagcc aattaagtgc
    37741 tgaacgcagc ttcgccagcc tgagtttgcg tgaagtggcg cgtgaagcgg gcattgctcc
    37801 cacctctttt tatcggcatt tccgagacgt agacgaactg ggtctgacca tggttgatga
    37861 gagcggttta atgctacgcc aactcatgcg ccaggcgcgt cagcgtatcg ccaaaggcgg
    37921 gagtgtgatc cgcacctcgg tctccacatt tatggagttc atcggtaata atcctaacgc
    37981 cttccggtta ttattgcggg aacgctccgg cacctccgct gcgtttcgtg ccgccgttgc
    38041 gcgtgaaatt cagcacttca ttgcggaact tgcggactat ctggaactcg aaaaccatat
    38101 gccgcgtgcg tttactgaag cgcaagccga agcaatggtg acaattgtct tcagtgcggg
    38161 tgccgaggcg ttggacgtcg gcgttgaaca acgtcggcaa ttagaagagc gactggtact
    38221 gcaactgcga atgatttcga aaggggctta ttactggtat cgccgtgaac aagagaaaac
    38281 cgcaattatt ccgggaaatg tgaaggacga gtaatgaaac aagcaaatca agatagaggt
    38341 acgctgctgc tggcgttggt tgctggctta tcgattaatg gtactttcgc agcgctgttt
    38401 agctccattg tgccattttc tgtattcccg attatttccc tggtgctgac ggtttactgc
    38461 ctgcatcaac gttatcttaa tcgcaccatg ccggtaggct tgccgggtct ggcagctgcc
    38521 tgttttattc tcggcgtact gctgtacagc acggtagttc gtgcggaata tccggatatc
    38581 ggctctaact tcttcccggc agtactctcc gtcattatgg tgttctggat tggcgcgaag
    38641 atgcgtaacc gtaagcagga agttgctgag taatcggtat catgccggat gctggcgcat
    38701 ccggcatggg ttttacttcg cggtcagtaa tacgctgcac tccatatggt gcgtgtaggg
    38761 gaactgatca aacagagcca gacgttcgac cttgtgcgtc tggcttaatg tttccagatt
    38821 tttgcataac gtttccggat tgcaggagat atacagaata cgtggatacg cctgcaccat
    38881 tttctcggtt tcactgtcca gaccgctgcg aggtgggtcg acaaaaatgg tttcgcactg
    38941 ataactcttt aagtcgatcc cttgcaggcg gttaaactcg cgcacgccat tcatcgcctg
    39001 agtaaattct tctgccgcca tacgaataat ttgtacgtta tcaatatggt tagcggcaat
    39061 gttgtattgc gcggcagcga ctgatggctt ggcgatttcg gtggctaata cccgatcaaa
    39121 attgcgcgcc agcgctaatg agaagttacc gttgccgcag tacagctcca gtaaatcgcc
    39181 ttttgagcct ttggttacgt ccagcgccca ttccagcatc tgaatattca tcgccgcgtt
    39241 cggctgggta aagctgtttt ctacctgacg atagatcatc tctttccctg cgaccggcag
    39301 acgctcgtcg atgtaatctt gatccagcgc gattttggtt ttcgttgccc gaccaatcag
    39361 atgcacattc agattctgcg cgcgcagtgc atcgcgcagg gcttccgctt cctgacgcca
    39421 ctcatcatcc agctttttat ggtacagcag ggaaaccacc gcctgattac tcagcgtggt
    39481 gaggtaatca atctggaaca acttgtggcg cagaacggga ttattacgca cacccgcaat
    39541 catcgccgtc atcaactggt tgatgagttc actggcggcg gggaagctat ccacccggat
    39601 acggcttttg gtttgttgat cgaaaatgat gtggtacagg tcatcgccat cgtgccagat
    39661 gcggaactcc gcacgcatcc ggtaatgact gaccggcgag cgaaacactt ccggaaccag
    39721 gtcagaaaac ggtgccatca tactttgcaa acgtaccact ttttcggcta actgcgcttc
    39781 atactgttct gttggaaggt gttcgggggt catgatgtat cctgaaaaat taaagtacgc
    39841 ggcgattgta gggattgctc atcagatgtc cagatcttga tgaattccta tttgtgagct
    39901 acgtctggac agtaacttgt tacaacctgt agcatccact tgccggtcct gtgagttaat
    39961 agggaatcca gtgcgaatct ggagctgacg cgcagcggta aggaaaggtg cgatgattgc
    40021 gttatgcgga cactgccatt cggtgggaag tcatcatctc ttagtatctt agatacccct
    40081 ccaagcccga agacctgccg gccaacgtcg catctggttc tcatcatcgc gtaatattga
    40141 tgaaacctgc ggcatccttc ttctattgtg gatgctttac aatgattaaa aaagcttcgc
    40201 tgctgacggc gtgttctgtc acagcctttt cctcttgggc acaggatacc agcccggata
    40261 ctctcgtcgt tactgctaac cgttttgaac agccgcgcag cactgtgctt gcaccaacca
    40321 ccgttgtgac gcgtcaggat atcgaccgct ggcagtcgac ctcggttaat gatgtgctgc
    40381 gccgtcttcc gggcgtcgat atcacccaaa acggcggttc aggtcagctc tcatctattt
    40441 ttattcgcgg tacaaatgcc agtcatgtgt tggtgttaat tgatggcgta cgcctgaatc
    40501 tggcgggggg gagtggttct gccgacctta gccagttccc tattgcgctt gtccagcgtg
    40561 ttgaatatat ccgtgggcca cgctccgccg tttatggttc cgatgcaata ggtggggtgg
    40621 tgaatatcat cacgacgcgc gatgaacccg gaacggaaat ttcagcaggg tggggaagca
    40681 atagttatca aaactatgat gtctctacac agcaacaact gggggataag acacgagtaa
    40741 cgttgttggg cgattatgcc catactcatg gttatgatgt tgttgcctat ggtaataccg
    40801 gaacgcaagc gcagccagat aacgatggtt ttttaagtaa aacgctttat ggcgcgctgg
    40861 agcataactt tactgatgcc tggagcggct ttgtgcgcgg ctatggctat gataaccgta
    40921 ccaattatga cgcgtattat tctccgggtt caccattggt cgatacccgt aaactctata
    40981 gtcaaagttg ggacgccggg ctgcgatata acggcgaact gattaaatca caactcatta
    41041 ccagctatag ccatagcaaa gattacaact acgatcccca ttatggtcgt tatgattcgt
    41101 cggcgacgct cgatgagatg aagcaataca ccgtccagtg ggcaaacaac atcatcattg
    41161 gccacggtaa tgttggtgcg ggtgttgact ggcagaagca gagcacggca ccgggcacag
    41221 cttatgttaa ggatggatat gatcaacgta ataccggcat ctatctgacc gggctgcaac
    41281 aagtcggcga ttttaccttt gaaggcgcag cacgcagcga cgataactca cagtttggtc
    41341 gtcatggaac ctggcaaacc agcgccggtt gggaattcat cgaaggttat cgcttcattg
    41401 cttcctacgg gacatcttat aaggcaccaa atctggggca actgtatggc ttctacggaa
    41461 atccgaatct ggacccggag aaaagcaaac agtgggaagg cgcgtttgaa ggcttaaccg
    41521 ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt cagtgacttg atcgattatg
    41581 atgatcacac cctgaaatat tacaacgaag ggaaagcgcg gattaagggc gtcgaggcga
    41641 ccgccaattt tgataccgga ccactgacgc atactgtgag ttatgattat gtcgatgcgc
    41701 gcaatgcaat taccgacacg ccgttgttac gccgtgctaa acagcaggtg aaataccagc
    41761 tcgactggca gttgtatgac ttcgactggg gtattactta tcagtattta ggcactcgct
    41821 atgataagga ttactcatct tatccttatc aaaccgttaa aatgggcggt gtgagcttgt
    41881 gggatcttgc ggttgcgtat ccggtcacct ctcacctgac agttcgtggt aaaatagcca
    41941 acctgttcga caaagattat gagacagtct atggctacca aactgcagga cgggaataca
    42001 ccttgtctgg cagctacacc ttctgaacca cgtcccaccg tgctggtgtt tgactccggc
    42061 gtcggtgggt tgtcggtcta tgacgagatc cggcatctct taccggatct ccattacatt
    42121 tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa gcgaagcgtt tattgttgag
    42181 cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc cccttgcgct ggctgtggtc
    42241 gcttgcaaca ctgccagtac cgtttcactt cctgcattac gcgaaaagtt cgacttcccg
    42301 gttgttggtg tcgtgccggc gattaaacct gctgcacgtc tgacggcaaa tggcattgtc
    42361 ggattactgg caacccgcgg aacagttaaa cgttcttata ctcatgagct gatcgcgcgt
    42421 ttcgctaatg aatgccagat agaaatgctg ggctcggcag agatggttga gttggctgaa
    42481 gcgaagctgc atggcgaaga tgtttctctg gatgcactaa aacgtatcct acgcccgtgg
    42541 ttaagaatga aagagccgcc agataccgtt gtattgggtt gcacccattt ccctctacta
    42601 caagaagaac tgttacaagt gctgccagag ggaacccggc tggtggattc tggcgcagcg
    42661 attgctcgcc gaacggcctg gttgttagaa catgaagccc cggatgcaaa atctgccgat
    42721 gcgaatattg ccttttgtat ggcaatgacg ccagaagctg aacaattatt gcccgtttta
    42781 cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt taggctgatt tggttgaatg
    42841 ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag gccggaataa ctccctataa
    42901 tgcgccacca ctgacacgga acaacggcaa acacgccgcc gggtcagcgg ggttctcctg
    42961 agaattccga cagagaaagc aaaaataaat gcttgactct gtagc
//
LOCUS       DABSYT020000029        42763 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.029, whole genome shotgun sequence.
ACCESSION   DABSYT020000029 DABSYT020000000
VERSION     DABSYT020000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 42763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.029"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(185..1945)
                     /gene="ycaO"
                     /locus_tag="ID725_RS09895"
                     /old_locus_tag="pgaptmp_001979"
     CDS             complement(185..1945)
                     /gene="ycaO"
                     /locus_tag="ID725_RS09895"
                     /old_locus_tag="pgaptmp_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGV7850588.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            2075..2767
                     /locus_tag="ID725_RS09900"
                     /old_locus_tag="pgaptmp_001980"
     CDS             2075..2767
                     /locus_tag="ID725_RS09900"
                     /old_locus_tag="pgaptmp_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGV7850589.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2966..4054
                     /gene="serC"
                     /locus_tag="ID725_RS09905"
                     /old_locus_tag="pgaptmp_001981"
     CDS             2966..4054
                     /gene="serC"
                     /locus_tag="ID725_RS09905"
                     /old_locus_tag="pgaptmp_001981"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGV7850590.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4125..5408
                     /gene="aroA"
                     /locus_tag="ID725_RS09910"
                     /old_locus_tag="pgaptmp_001982"
     CDS             4125..5408
                     /gene="aroA"
                     /locus_tag="ID725_RS09910"
                     /old_locus_tag="pgaptmp_001982"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGV7850591.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5577..6341
                     /gene="ycaL"
                     /locus_tag="ID725_RS09915"
                     /old_locus_tag="pgaptmp_001983"
     CDS             5577..6341
                     /gene="ycaL"
                     /locus_tag="ID725_RS09915"
                     /old_locus_tag="pgaptmp_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGV7850592.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6514..7197
                     /gene="cmk"
                     /locus_tag="ID725_RS09920"
                     /old_locus_tag="pgaptmp_001984"
     CDS             6514..7197
                     /gene="cmk"
                     /locus_tag="ID725_RS09920"
                     /old_locus_tag="pgaptmp_001984"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGV7850593.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7308..8981
                     /gene="rpsA"
                     /locus_tag="ID725_RS09925"
                     /old_locus_tag="pgaptmp_001985"
     CDS             7308..8981
                     /gene="rpsA"
                     /locus_tag="ID725_RS09925"
                     /old_locus_tag="pgaptmp_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGV7850594.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9141..9425
                     /gene="ihfB"
                     /locus_tag="ID725_RS09930"
                     /old_locus_tag="pgaptmp_001986"
     CDS             9141..9425
                     /gene="ihfB"
                     /locus_tag="ID725_RS09930"
                     /old_locus_tag="pgaptmp_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGV7850595.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9632..11896
                     /locus_tag="ID725_RS09935"
                     /old_locus_tag="pgaptmp_001987"
     CDS             9632..11896
                     /locus_tag="ID725_RS09935"
                     /old_locus_tag="pgaptmp_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGV7850596.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11933..13681
                     /gene="msbA"
                     /locus_tag="ID725_RS09940"
                     /old_locus_tag="pgaptmp_001988"
     CDS             11933..13681
                     /gene="msbA"
                     /locus_tag="ID725_RS09940"
                     /old_locus_tag="pgaptmp_001988"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGV7850597.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13678..14664
                     /gene="lpxK"
                     /locus_tag="ID725_RS09945"
                     /old_locus_tag="pgaptmp_001989"
     CDS             13678..14664
                     /gene="lpxK"
                     /locus_tag="ID725_RS09945"
                     /old_locus_tag="pgaptmp_001989"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGV7850598.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14701..15933
                     /gene="ycaQ"
                     /locus_tag="ID725_RS09950"
                     /old_locus_tag="pgaptmp_001990"
     CDS             14701..15933
                     /gene="ycaQ"
                     /locus_tag="ID725_RS09950"
                     /old_locus_tag="pgaptmp_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGV7850599.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15985..16167
                     /gene="ycaR"
                     /locus_tag="ID725_RS09955"
                     /old_locus_tag="pgaptmp_001991"
     CDS             15985..16167
                     /gene="ycaR"
                     /locus_tag="ID725_RS09955"
                     /old_locus_tag="pgaptmp_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGV7850600.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16164..16910
                     /gene="kdsB"
                     /locus_tag="ID725_RS09960"
                     /old_locus_tag="pgaptmp_001992"
     CDS             16164..16910
                     /gene="kdsB"
                     /locus_tag="ID725_RS09960"
                     /old_locus_tag="pgaptmp_001992"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGV7850601.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            17064..17957
                     /locus_tag="ID725_RS09965"
                     /old_locus_tag="pgaptmp_001993"
     CDS             17064..17957
                     /locus_tag="ID725_RS09965"
                     /old_locus_tag="pgaptmp_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGV7850602.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17934..18713)
                     /gene="elyC"
                     /locus_tag="ID725_RS09970"
                     /old_locus_tag="pgaptmp_001994"
     CDS             complement(17934..18713)
                     /gene="elyC"
                     /locus_tag="ID725_RS09970"
                     /old_locus_tag="pgaptmp_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGV7850603.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18849..19634
                     /gene="cmoM"
                     /locus_tag="ID725_RS09975"
                     /old_locus_tag="pgaptmp_001995"
     CDS             18849..19634
                     /gene="cmoM"
                     /locus_tag="ID725_RS09975"
                     /old_locus_tag="pgaptmp_001995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGV7850604.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19631..20953
                     /gene="mukF"
                     /locus_tag="ID725_RS09980"
                     /old_locus_tag="pgaptmp_001996"
     CDS             19631..20953
                     /gene="mukF"
                     /locus_tag="ID725_RS09980"
                     /old_locus_tag="pgaptmp_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGV7850605.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20934..21638
                     /gene="mukE"
                     /locus_tag="ID725_RS09985"
                     /old_locus_tag="pgaptmp_001997"
     CDS             20934..21638
                     /gene="mukE"
                     /locus_tag="ID725_RS09985"
                     /old_locus_tag="pgaptmp_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGV7850606.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21638..26098
                     /gene="mukB"
                     /locus_tag="ID725_RS09990"
                     /old_locus_tag="pgaptmp_001998"
     CDS             21638..26098
                     /gene="mukB"
                     /locus_tag="ID725_RS09990"
                     /old_locus_tag="pgaptmp_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGV7850607.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26359..28206
                     /gene="ldtD"
                     /locus_tag="ID725_RS09995"
                     /old_locus_tag="pgaptmp_001999"
     CDS             26359..28206
                     /gene="ldtD"
                     /locus_tag="ID725_RS09995"
                     /old_locus_tag="pgaptmp_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGV7850608.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28387..28935
                     /locus_tag="ID725_RS10000"
                     /old_locus_tag="pgaptmp_002000"
     CDS             28387..28935
                     /locus_tag="ID725_RS10000"
                     /old_locus_tag="pgaptmp_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGV7850609.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28962..29609
                     /gene="gloC"
                     /locus_tag="ID725_RS10005"
                     /old_locus_tag="pgaptmp_002001"
     CDS             28962..29609
                     /gene="gloC"
                     /locus_tag="ID725_RS10005"
                     /old_locus_tag="pgaptmp_002001"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGV7850610.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29660..30850)
                     /gene="aspC"
                     /locus_tag="ID725_RS10010"
                     /old_locus_tag="pgaptmp_002002"
     CDS             complement(29660..30850)
                     /gene="aspC"
                     /locus_tag="ID725_RS10010"
                     /old_locus_tag="pgaptmp_002002"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGV7850611.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31035..32123)
                     /gene="ompF"
                     /locus_tag="ID725_RS10015"
                     /old_locus_tag="pgaptmp_002003"
     CDS             complement(31035..32123)
                     /gene="ompF"
                     /locus_tag="ID725_RS10015"
                     /old_locus_tag="pgaptmp_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGV7850612.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(32726..34126)
                     /gene="asnS"
                     /locus_tag="ID725_RS10020"
                     /old_locus_tag="pgaptmp_002004"
     CDS             complement(32726..34126)
                     /gene="asnS"
                     /locus_tag="ID725_RS10020"
                     /old_locus_tag="pgaptmp_002004"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGV7850613.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(34295..35497)
                     /gene="pncB"
                     /locus_tag="ID725_RS10025"
                     /old_locus_tag="pgaptmp_002005"
     CDS             complement(34295..35497)
                     /gene="pncB"
                     /locus_tag="ID725_RS10025"
                     /old_locus_tag="pgaptmp_002005"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGV7850614.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            35763..38375
                     /gene="pepN"
                     /locus_tag="ID725_RS10030"
                     /old_locus_tag="pgaptmp_002006"
     CDS             35763..38375
                     /gene="pepN"
                     /locus_tag="ID725_RS10030"
                     /old_locus_tag="pgaptmp_002006"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGV7850615.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(38418..39185)
                     /gene="ssuB"
                     /locus_tag="ID725_RS10035"
                     /old_locus_tag="pgaptmp_002007"
     CDS             complement(38418..39185)
                     /gene="ssuB"
                     /locus_tag="ID725_RS10035"
                     /old_locus_tag="pgaptmp_002007"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGV7850616.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(39182..39973)
                     /gene="ssuC"
                     /locus_tag="ID725_RS10040"
                     /old_locus_tag="pgaptmp_002008"
     CDS             complement(39182..39973)
                     /gene="ssuC"
                     /locus_tag="ID725_RS10040"
                     /old_locus_tag="pgaptmp_002008"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGV7850617.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(39985..41130)
                     /gene="ssuD"
                     /locus_tag="ID725_RS10045"
                     /old_locus_tag="pgaptmp_002009"
     CDS             complement(39985..41130)
                     /gene="ssuD"
                     /locus_tag="ID725_RS10045"
                     /old_locus_tag="pgaptmp_002009"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGV7850618.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            complement(41127..42086)
                     /gene="ssuA"
                     /locus_tag="ID725_RS10050"
                     /old_locus_tag="pgaptmp_002010"
     CDS             complement(41127..42086)
                     /gene="ssuA"
                     /locus_tag="ID725_RS10050"
                     /old_locus_tag="pgaptmp_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGV7850619.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(42079..42654)
                     /gene="ssuE"
                     /locus_tag="ID725_RS10055"
                     /old_locus_tag="pgaptmp_002011"
     CDS             complement(42079..42654)
                     /gene="ssuE"
                     /locus_tag="ID725_RS10055"
                     /old_locus_tag="pgaptmp_002011"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGV7850620.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
BASE COUNT        10504 a        10664 c        11325 g        10270 t
ORIGIN      
        1 gggagcatca ggcgaataaa gtaacaatat cgatcgtatt tattaaatac aaattaccga
       61 tatttaacct tataattaca attattttat taatgcaaat atatgtaaag cggggcatta
      121 aaaaaacgcc ccgtaatata actcagacga atcattaagc ctacattacg taggctattt
      181 gattttattt tgcccagaat gctgctttgg cgcgctgcag cttttcgtag gccttcaaca
      241 gcgactgatg tgcagcaaac gcgtgcagat cgctatctac tggttgcaag ccataaaacg
      301 ccgcttcgcc gctcattgcc gcactggcgg cttccacggc atctgcgccg tacatgcgaa
      361 caaacgcatt cagatattgc agcggctggc gatcttcttc ctgcgccagt aataacaacg
      421 tttgcaggca gcgataatag ttggcgcgtt ccgggctgaa taccgatgag ttaaactcca
      481 tcgtccattc ggtccagacc agagcctgtt ccagatcgcc accagccagc gccagcatgg
      541 cttttaattc accaatacgc agggtgtacc aaccgttatc cgacccggtc gccagaccca
      601 acagctcacg cacgcgggta aagtcatcaa aaccttcttc atccagttgc tcgatgaggt
      661 tcaggtaatc ttctttttcc cactcgctgc ctggtagcga aagaatcgtt tcacgtaaat
      721 ggctgcccat actgttattc gcgagccaca gatcttcagc cggataaata tcggacatgc
      781 caggcacgat aatacggcaa gcataaacgc ccagatgctc gtaatcggca atataaactt
      841 ctttatcttc tttgttgaag atagccatca gcgtagcaaa ctcttcttcc gtggtgccgg
      901 agaaattcca gtccacaaac ggataatcgg catcctgctt gaacaggtcc caggagatta
      961 aaccgctgga atcgataaag tgcgtttcga ggttggtatg ttcagcgact tcttcatcat
     1021 cgaaggttgg cggagtaaac acatccaaat ctttcaggcc acgaccttgc agcagctcgg
     1081 tcacggtacg ttccagtgct acgccaaaat caggatgcgc accgaaagag gcaaagcagg
     1141 tgccgttagc aggattaaac agtaccacgc aaatcaccgg atactggccg ccaagggaac
     1201 catcatatgc gaagatcggg aaaccttctg cttccagtgt ttcgatcgct tcaaccaccg
     1261 ctgggtaacg cgccagcacg tctgccggga tttccggcaa gctgatgctt tcagcaataa
     1321 tgcggttttt cacgtagcgt tcgaaaactt cggacaaccc ctgaacgcgt gcttcgttac
     1381 gggtattgcc agcagacata ccgttagaaa cgtacaggtt accaatgata ttcatcggaa
     1441 tataaacggt ctgattatca gactgacgtg taaacggcag accgcaaata ccacgatctt
     1501 cgttaccgga ttgtaggtca atcagcatac tgccggtcaa ttcattctcc ggatcgtaaa
     1561 atgcgcgcag acgttcatcg agcagccctt ctggcacatc gtcattttcg gtcagtggga
     1621 accatttttc gttgggataa tgcacaaatg gaccgttggc gatggtttcg cccagccaga
     1681 aatcagcaaa aaagtagttg gttgagagac gctcgaaata ttcaccgagt gcagaagcca
     1741 gcgccgcttt cttggttgcg cctttaccgt tggtaaaaca cagtgcgcac tctttgtcgc
     1801 gaatatgtac agaccagacg ttaggcacgg gattcagcca ggaggcctct tcaatctgaa
     1861 agccgaggtc tgaaagtttt tgctggaagc gagcgatgga atcttccagt gcggcatctt
     1921 tgccggggat aaatgtttgc gtcatgaaaa tcactttagt cgtacggaaa gcgcgcaata
     1981 atacgggttt tatctcaaag gcgctatcac cgccgccatg ccgatgaact gttgactatg
     2041 cttttagcgg ataaaccacg ttaagagcat aaaaatgaag gcgttcgatc tccaccgtat
     2101 ggcatttgat aaagtgcctt ttgatttcct tggcgaagtt gcactacgta gtctttatac
     2161 ctttgtactg gtctttttgt tcctcaaaat gaccggaaga cgcggtgtgc ggcagatgtc
     2221 gctgtttgaa gttttaatca ttctgacgct gggatcagcg gcgggagatg tggcgtttta
     2281 tgatgatgtg ccgatggtcc cggtacttat cgtctttatt actctggcgt tgttataccg
     2341 cctggtaatg tggttgatgg cgcacagtga aaaactggaa gatcttctgg aaggcaagcc
     2401 agttgtcatt attgaagatg gcgagctggc ctggtcgaaa ctcaataact ccaacatgac
     2461 ggaatttgag ttctttatgg agctacgatt gcgtggcgtg gagcagctgg ggcaggtacg
     2521 tctggcgatt ctcgaaacca acgggcaaat cagtgtctat ttctttgaag atgacaaggt
     2581 gaaatcgggt ttacttattt tacccagtga ttgtacgcag cgttacaaag tggtgccgga
     2641 gtcggcggac tatgcctgta ttcgttgtag tgaaatcatt catatgaaag cgggggaaaa
     2701 acaattatgt ccgcgctgtg caaatccaga atggacgaag gcaagtcggg caaaacgggt
     2761 gacctgacag taaaaacatc ggctttttgc taataatccg agagattctt ttgtgtgatg
     2821 caagccacat ttttgccctc aacggtttta ctcattgcga tgtgtgtcac tgaatgataa
     2881 aaccgatagc cacaggaata atgtattacc tgtggtcgca atcgattgac cgcgggttaa
     2941 tagcaacgca acgtggtgag gggaaatggc tcaaatcttc aattttagtt ctggtccggc
     3001 aatgctaccg gtagaggtgc ttaaacaggc tcaacaggaa ctgcgcgact ggaacggtct
     3061 tggtacgtcg gtgatggaag tgagtcaccg tggcaaagag ttcattcagg ttgcagagga
     3121 agccgagaag gattttcgcg atcttcttaa tgtcccctcc aactacaagg tattattctg
     3181 ccatggcggt ggtcgcggtc agtttgctgc ggtaccgctg aatattctcg gtgataaaac
     3241 caccgcagat tatgttgatg ccggttactg ggcggcaagt gccattaaag aagcgaaaaa
     3301 atactgcacg cctaatgtct ttgacgccaa agtgactgtt gatggtctgc gcgcggttaa
     3361 gccaatgcgt gaatggcaac tctctgataa tgctgcttat atgcattact gcccgaatga
     3421 aaccatcgac ggtatcgcca tcgacgaaac gccagacttc ggcaaagatg tggtggtcgc
     3481 cgccgacttc tcttcaacca ttctttcccg tccgattgac gtcagccgtt atggtgtaat
     3541 ttacgctggc gcgcagaaaa atatcggccc ggcaggcctg acaatcgtca tcgtccgtga
     3601 agatttgctg ggcaaagcga atgtcgcgtg tccgtcgatt ctggattatt ccatcctcaa
     3661 cgataacggc tccatgttta acacgccgcc gacatttgcc tggtatctat ctggtctggt
     3721 ctttaaatgg ctgaaagcga acggcggtgt agctgaaatg gataaaatca atcagcaaaa
     3781 agcagaactg ctatatgggg tgattgataa cagcgatttc taccgcaatg acgtggcgaa
     3841 agctaaccgt tcgcggatga acgtgccgtt ccagttggcg gacagtgcgc ttgacaaatt
     3901 gttccttgaa gagtcttttg ctgcaggcct tcatgcactg aaaggtcacc gtgtggtcgg
     3961 cggaatgcgc gcgtctattt ataatgccat gccgctggaa ggcgttaaag cgctgacaga
     4021 cttcatggtt gagttcgaac gccgtcacgg ttaatgccga aattttgctt aatccccaca
     4081 gccagcctgt ggggttttta tttctgttgt agagagttga gttcatggaa tccctgacgt
     4141 tacaacccat cgctcgtgtc gatggcacta ttaatctgcc cggttccaag agcgtttcta
     4201 accgcgcttt attgctggcg gcattagcac acggcaaaac agtattaacc aatctgctgg
     4261 atagcgatga cgtgcgccat atgctgaatg cattaacagc gttaggggta agctatacgc
     4321 tttcagccga tcgtacacgt tgcgaaatta tcggtaacgg cggtccatta cacgcagaag
     4381 gtgccctgga gttgttcctc ggtaacgccg gaacggcaat gcgtccgctg gcggcagctc
     4441 tttgtctggg tagcaatgat attgtgctga ccggtgagcc gcgtatgaaa gagcgcccga
     4501 ttggtcatct ggtggatgcg ctgcgcctgg gcggggcgaa gatcacttac ctggaacaag
     4561 aaaattatcc gccgttgcgt ttacagggcg gctttactgg cggcaacgtt gacgttgatg
     4621 gctccgtttc cagccaattc ctcaccgcac tgttaatgac tgcgcctctt gcgccggaag
     4681 atacggtgat tcgtattaaa ggcgatctgg tttctaaacc ttatatcgac atcacactca
     4741 atctgatgaa gacgtttggt gttgaaattg aaaatcagca ctatcaacaa tttgtcgtaa
     4801 aaggcgggca gtcttatcag tctccgggta cttatttggt cgaaggcgat gcatcttcgg
     4861 cttcttactt tctggcagca gcagcaatca aaggcggcac tgtaaaagtg accggtattg
     4921 gacgtaacag tatgcagggt gatattcgct ttgctgatgt gctggaaaaa atgggcgcga
     4981 ccatttgctg gggcgatgat tatatttcct gcacgcgtgg tgaactgaac gctattgata
     5041 tggatatgaa ccatattcct gatgcggcga tgaccattgc tacggcggcg ttatttgcaa
     5101 aaggcaccac cacgctgcgc aatatctata actggcgtgt taaagaaacc gatcgcctgt
     5161 ttgcgatggc aacagaactg cgtaaagtcg gtgcggaagt agaagagggg cacgattaca
     5221 ttcgtatcac tccaccggaa aaactgaatt ttgccgagat cgcgacatac aatgatcacc
     5281 ggatggcgat gtgtttctcg ctggtggcgt tgtcagatac accagtgacg attcttgatc
     5341 ccaaatgtac ggccaaaaca tttccggatt atttcgagca gctggcgcgg attagccagg
     5401 cagcctgaat gaataacgtc caataaatag ccaaaacttt ctttatcaaa acgtcggcac
     5461 attgccggcg ttttttttcg gaccttgtga gtcattttga ttaatggtag cgtcgcttgt
     5521 caatgtaagt tgttgataca taatgtttat atatgattaa tcaacggatg attcacatga
     5581 agaatactaa attactgctg gcgattgtga cctctgcagc attactgacg gggtgtcaaa
     5641 atacccacgg tattgatacc aatatggcta tcagctccgg tttaaatgcc tataaagcag
     5701 caacgttaag cgatgccgat gcaaaagcga ttgccaatca gggctgtgcc gaaatggaca
     5761 gcggcaatca agtcgcaagt aaatccagca agtacggtaa acgtctggca aaaatcgcca
     5821 aagcattggg taacaatatt aatggcacgc cggtcaacta taaggtttat atgaccagcg
     5881 acgtcaacgc atgggcgatg gcgaacggct gtgttcgtgt ttacagtggc ctgatggaca
     5941 tgatgaacga taacgaaatt gaaggcgttc tgggccatga actgggccac gtcgcgttgg
     6001 gtcactcgct ggctgaaatg aaagcttctt atgcgatcgt tgccgcacgc gatgccattt
     6061 cagctaccag cggtgtggct tcccagcttt cccgctcaca attgggtgat atcgcagaag
     6121 gcgctatcaa tgcgaaatac tcccgcgata aagagtccga agcagatgat ttctcctttg
     6181 atctgttgaa gaaacgtggc atcagcaccc aggggctggt tggcagcttt gaaaaactgg
     6241 ctagcctgga tggcggtcgc acccagtcca tgtttgactc tcacccacca tcaacagagc
     6301 gtgcgcaaca catccgtgat cgtatcgcct ctggtaagta aatcattgtc atctttcggg
     6361 ctggtcttct gccagcccgc tataattgcg caataaatcc ccatctgaat acagacaaaa
     6421 ctggtttttg cacacaacgt taacgatttg tggcgtcggc gcgtataatg cgcgcggtta
     6481 tgttaacggt acgcctgttt taaggagata aagatgacgg caattgcccc ggttattacc
     6541 attgatggcc caagcggtgc agggaaaggc accttgtgta aggctatggc ggaagcgttg
     6601 caatggcatc tgctggattc gggtgcaatt tatcgcgtac tggcactagc ggcattacat
     6661 caccatgttg atgttgcatc ggaagatgcg ctggtgccgc tggcatccca tctggatgta
     6721 cgttttgtgt caaccaatgg caatctggaa gtgatcctcg aaggggaaga tgtcagcggc
     6781 gaaattcgta ctcaggaagt ggcgaatgca gcttcacaag tcgcggcatt cccacgcgtt
     6841 cgtgaagcat tattgcgtcg ccaacgcgcg tttcgcgaat taccaggcct gattgccgat
     6901 ggccgcgaca tgggaacggt ggtattccct gatgcgccag tgaaaatttt ccttgacgcc
     6961 tcctcggaag aacgtgcgca tcgccgcatg ctacagttgc aggagaaggg ctttagtgtt
     7021 aactttgagc gccttttggc cgagatcaaa gaacgcgacg accgcgatcg taaccgagcg
     7081 gtagcgccac tggttccggc agccgatgct ttagtgttgg attccaccac cttaagcatt
     7141 gagcaagtga ttgaaaaagc gctacaatac gcgcgccaga aattggctct cgcataagcg
     7201 accgaatttg cagtaccccc gttgcaatgg aatgacagcg ggtatgttaa acaaccccat
     7261 ccggcatgga gccaggtgga cgttaaatat aaacctgaag attaaacatg actgaatctt
     7321 ttgctcaact ctttgaagag tccttaaaag aaatcgaaac ccgcccgggt tctatcgttc
     7381 gtggcgttgt tgttgctatc gacaaagacg tagtactggt tgacgctggt ctgaaatctg
     7441 agtccgccat cccggctgag cagttcaaaa acgcccaggg cgagctggaa atccaggtag
     7501 gtgacgaagt tgacgttgct ctggacgcag tagaagacgg cttcggtgaa actctgctgt
     7561 cccgtgagaa agctaaacgt cacgaagcct ggatcacgct ggaaaaagct tacgaagatg
     7621 ctgaaactgt taccggtgtt atcaacggca aagttaaggg cggcttcact gttgagctga
     7681 acggtattcg tgcgttcctg ccaggttctc tggtagacgt tcgtccggtg cgtgacactc
     7741 tgcacctgga aggcaaagag cttgaattca aagtaatcaa gctggatcag aagcgcaaca
     7801 acgttgttgt ttctcgtcgt gccgttatcg aatccgaaaa cagcgcagag cgcgatcagc
     7861 tgctggaaaa cctgcaggaa ggcatggaag ttaaaggtat cgttaagaac ctcactgact
     7921 acggtgcatt cgttgatctg ggcggcgttg acggcctgct gcacatcact gacatggcct
     7981 ggaaacgcgt taagcatccg agcgaaatcg tcaacgtggg cgacgaaatc actgttaaag
     8041 tgctgaagtt cgaccgcgaa cgtacccgtg tatccctggg cctgaaacag ctgggcgaag
     8101 atccgtgggt agctatcgct aaacgttatc cggaaggtac caaactgact ggtcgcgtga
     8161 ccaacctgac cgactacggc tgcttcgttg aaatcgaaga aggcgttgaa ggcctggtac
     8221 acgtttccga aatggattgg accaacaaaa acatccaccc gtccaaagtt gttaacgttg
     8281 gcgatgtagt ggaagttatg gttctggata tcgacgaaga acgtcgtcgt atctccctgg
     8341 gtctgaaaca gtgcaaagct aacccgtggc agcagttcgc ggaaacccac aacaagggcg
     8401 accgtgttga aggtaaaatc aagtctatca ctgacttcgg tatcttcatc ggcctggacg
     8461 gcggcatcga cggcctggtt cacctgtctg acatctcctg gaacgttgca ggcgaagaag
     8521 cagttcgtga atacaaaaaa ggcgacgaaa tcgctgcagt tgttctgcag gttgacgcag
     8581 aacgtgaacg tatctccctg ggcgttaaac agctcgcaga agatccgttc aacaactggg
     8641 ttgctctgaa caagaaaggc gctatcgtaa ccggtaaagt aactgcagtt gacgctaaag
     8701 gcgcaaccgt agaactggct gacggcgttg aaggttacct gcgtgcttct gaagcatccc
     8761 gtgaccgcgt tgaagacgct accctggttc tgagcgttgg cgacgaagtt gaagctaaat
     8821 tcaccggcgt tgatcgtaaa aaccgtgcaa tcagcctgtc tgttcgtgcg aaagacgaag
     8881 ctgacgagaa agatgcaatc gcaactgtta acaaacagga agatgcaaac ttctccaaca
     8941 acgcaatggc tgaagctttc aaagcagcta aaggcgagta attctctgac tcttcgggat
     9001 ttttattctg aagtttgttg agtttacttg acagattgca ggtttcgtcc tgtaatcaag
     9061 cactaagggc ggctacggcc gcccttaatc aatgcagcaa cagcagccgc ttaatttgcc
     9121 tttaaggaac cggaggaatc atgaccaagt cagaattgat agaaagactt gccacccagc
     9181 aatcgcacat tcccgccaag acggttgaag atgcagtaaa agagatgctg gagcatatgg
     9241 cctcgactct tgcgcagggc gagcgtattg aaatccgcgg tttcggcagt ttctctttgc
     9301 actaccgcgc accacgtacc ggacgtaatc cgaagactgg cgataaagta gaactggaag
     9361 gaaaatacgt tcctcacttt aaacctggta aagaactgcg cgatcgcgcc aatatttacg
     9421 gttaagtttt tactcaaact tgaacgagag aaaagcacct gtcgggtgct tttttcattt
     9481 atctaatctg gaactggaag ctgcctcgca gagttttgag cagttttcac cctttcgtta
     9541 aattcttctg aatgtgcctc ggggaacgca aaattcccac cagacaaccg ctcaacaaag
     9601 ttgcacactt tccataaaca gggaggggtg catgaaaata acgacagtcg gcgtatgcat
     9661 aatttgcgga atttttccgt tgctgatttt gccccaattg cctgggacat taacccttgc
     9721 gtttctgact ctcttcgcct gcgtactggc atttatccct gttaaaaccg tccgttatat
     9781 cgcgctgacg ttgctgtttt tcgtttgggg catattatca gcaaagcaaa ttttgttggc
     9841 aggagaaacc ttaactggcg cgacgcagga tgcaattgtt gagatcactg ctactgacgg
     9901 catgaccact cattacggtc aaattactta tctacaaggt caacgtatat tccctgcgcc
     9961 aggccttgtg ctgtatggcg aatatcttcc gcaagcggtt tgtgccggac aactgtggtc
    10021 aatgaaactc aaagttcgtg ccgttcatgg tcaacttaat gatggcggct ttgatagcca
    10081 gcgttatgcc attgcccagc atcagccgct caccggccgc tttctgcagg caagtgttat
    10141 tgaaccgaat tgtagcctgc gtgcacagta tctggcgtca ctacaaacaa cgctgcaacc
    10201 ctatccgtgg aatgcggtta ttcttggttt aggtatgggg gaacggttat ccgtccctaa
    10261 agaaatcaaa aatatcatgc gtgatactgg aacggcgcat ttaatggcga tatcgggatt
    10321 gcacatcgct tttgcggcgt tgctggctgc cggactcatt cgcagtggac aaatttttct
    10381 gcctgggcgc tggatccact ggcaaatgcc attaattggc ggaatctgct gtgctgcttt
    10441 ttatgcctgg ctgacgggaa tgcaacctcc tgcattgcgt accgttgtgg cgcttgctac
    10501 gtggggtatg cttaagttaa gtgggcgaca gtggagtggc tgggatgtat ggatatgttg
    10561 tctggcggca attttgctga tggatcctgt tgtcattctc tcgcaaagtt tatggctctc
    10621 tgccgctgcg gtcgcggcat tgatattttg gtatcagtgg tttccctgtc ctgagtggca
    10681 actgccgccg gtattgcgtg cagttgtttc cctcatccat ctgcaactgg gaatcacact
    10741 tctgcttatg cccgtgcaaa tcgtcatttt tcatggcatt agtctgacct cgtttattgc
    10801 aaatctatta gcaattccct tggtgacatt tatcacggtt ccgttgatcc tcgccgcgat
    10861 ggttgtgcat ttaagcgggc cgttaatcct ggatcaaggg ttatggtttc ttgccgaccg
    10921 gtctttggct ttacttttct tggggttaaa gagtttgcca gaagggtgga tcaacattgc
    10981 tgaacgttgg caatggctat cattttcccc atggttctta ctggtggtat ggcgattaaa
    11041 cgcctggcga acgttgccag caatgtgtgt ggctgtaggc ttgctgatgt gctggccgct
    11101 gtggcaaaaa cctcgacctg acgagtggca agtgtacatg cttgatgtcg ggcaagggct
    11161 ggcaatggtg atagccagaa acggcaaagc gattctctat gacacgggac tggcctggcc
    11221 tgaaggggat agtgggcaac aactgattat cccctggctc cactggcata atcttgaacc
    11281 ggaaggcgtt atcctgagtc atgaacatct ggatcaccgg ggagggctgg actcaatatt
    11341 gcatacatgg ccgatgttat ggatcagaag tccgttaaac tgggaacatc atcagccctg
    11401 tgtgcgtggc gaagcgtggc aatggcaagg attgcgtttc agcgcgcact ggcctttaca
    11461 aggtcgcaac gataaaggaa ataaccattc ctgtgtggtt aaggttgatg acgggacgaa
    11521 tagcattctt ctaaccggtg atattgaagc cccagctgaa caaaagatgc taagccgtta
    11581 ctggcagcaa gtgcaggcaa cattgcttca ggtacctcac catggcagta atacctcatc
    11641 atcattgcca ttaattcagc gagtgaatgg aaaagtggca ctcgcatcgg catcgcgcta
    11701 taacgcatgg cgactgccct ctaacaaagt taagcatcgc tatcaacaac aaggttatac
    11761 gtggcttgat actcctcatc aggggcaagt aacggtcgat ttttcagcgc aaggctggcg
    11821 gattagcagc ctcagggagc aaattttacc tcgttggtat catcagtggt ttggcgtgcc
    11881 agtggataac gggtagaata tgcggctatt tcaacaaatg ctggtttttt gaatgcataa
    11941 cgacaaagat ctctctacgt ggcagacatt ccgccgactg tggccaacca ttgcgccttt
    12001 taaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca gcgatacctt
    12061 catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag atcgctccgt
    12121 gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttgcgtggta tcaccagcta
    12181 tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg
    12241 cctgtttggt cacatgatgg gaatgccggt ttcattcttt gacaaacagt caacgggtac
    12301 gctgttgtca cgtatcacct acgattccga acaggttgct tcttcctctt ccggcgcact
    12361 gattactgtt gtgcgtgaag gtgcgtcgat catcggcctg ttcatcatga tgttctatta
    12421 cagttggcaa ttgtcgatca ttttgattgt gctggcaccg attgtttcga ttgcgattcg
    12481 cgtagtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca tggggcaggt
    12541 gaccaccagc gctgaacaaa tgctgaaagg ccataaagaa gtattgattt tcggtggtca
    12601 ggaagtggaa acgaaacgct tcgataaagt cagcaaccga atgcgtcttc aggggatgaa
    12661 aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct ctttggcgct
    12721 ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga ctgccggtac
    12781 gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat cgctgactaa
    12841 cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acactgttta ccattctgga
    12901 cagtgagcag gagaaagacg aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga
    12961 attccgcaac gtcaccttta cttatccggg acgtgacgta cccgcattgc gtaacatcaa
    13021 cctgaaaatt ccggcaggga aaacggtcgc tctggttgga cgctctggtt caggtaaatc
    13081 aaccatcgcc agcctgatca cgcgttttta cgatattgat gaaggcgaaa tcctgatgga
    13141 tggtcacgat ctgcgcgagt ataccctggc gtcgttgcgt aaccaggttg ctctggtgtc
    13201 gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg cacgaactga
    13261 acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat
    13321 caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtac tgctctctgg
    13381 cggtcagcgt cagcgtattg ctatcgctcg agccttgttg cgtgatagcc cgattctgat
    13441 tctggatgaa gctacctcgg ctctggatac cgaatccgaa cgtgcgattc aggcggcact
    13501 ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt ctaccattga
    13561 aaaggcagac gaaatcgtgg tcgtcgagga tggtgtcatt gtggaacgcg gtacgcataa
    13621 cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg
    13681 atcgaaaaaa tctggtctgg tgaatcccct ttgtggcggc tattgctgcc actctcctgg
    13741 ttgtatggcc tggtgagtgg cgcgatccgt ctttgctata aactaaaact gaagcgcgcc
    13801 tggcgtgccc ccgtaccggt tgtcgtggtt ggtaatctca ccgcaggcgg caacggaaaa
    13861 accccggtcg ttgtctggct ggtggaacag ttgcaacagc gcggtattcg cgtgggggtc
    13921 gtatcgcggg gatatggtgg taaggctgaa tcttatccgc tgttattgtc ggcagatacc
    13981 accacagcac aggcgggtga tgaacctgtg ttgatttatc aacgcactga tgcgcctgtt
    14041 gcggtttctc ccgttcgttc tgatgcggta aaagccattc tggcgcaaca ccctgatgtg
    14101 cagatcatcg taaccgacga cggtttacag cattaccgtc tggcgcgtga tgtggaaatt
    14161 gtcgttattg atggtgtgcg tcgctttggc aatggctggt ggttgccggc ggggccaatg
    14221 cgtgagcgag cggggcgctt aaagtcagtt gatgcggtaa tcgtcaacgg cggtgtcccc
    14281 cgcagcggtg aaatccccat gcatctgctg ccgggtcagg cggtgaattt acgtaccggt
    14341 acgcgttgtg acgttgctca gcttgaacat gtggtggcga tggcagggat tgggcatccg
    14401 ccgcgctttt ttgccacgct gaagatgtgc ggcgtacaac cggaaaaatg tgtaccgctg
    14461 gccgatcatc agtctttgaa ccatgcggat gtcagcgcgt tggtaagcac cgggcaaacg
    14521 ctggtaatga ctgaaaaaga tgcggtgaaa tgccgggcct ttgcagaaga aaattggtgg
    14581 tatttgcccg ttgacgcaca gctttcaggt gatgaaccag cgaaactgct tgcgcaacta
    14641 acctcgctgg cttctggcaa ctagttacgc cgcggcagcg ttcgattgat ggagtcatga
    14701 atgtcgctgc cgcacctctc ccttgctgat gcgcgtaatc ttcaccttgc cgcgcaaggc
    14761 ctgttaaaca aaccccgccg tcgagcgtcg ttggaggata ttccggcaac gatctcccgc
    14821 atgtccttgc tgcaaatcga taccatcaat attgttgccc gtagcccata tctggtgctt
    14881 ttcagtcgtc tgggaaatta tcctgcccag tggctggatg agtctctggc gcgtggcgaa
    14941 ttaatggaat actgggcgca tgaagcctgc tttatgccac gcagtgactt tcgtcttatt
    15001 cgccaccgca tgctggcacc tgaaaaaatg ggctggaaat acaaagacgc ctggatgcag
    15061 gaacatgagg cggaaattgc acagttaatt cagcatattc atgataaggg gccggtacgt
    15121 tcagccgatt ttgagtatcc tcgtaaaggt gcaagcggct ggtgggaatg gaagccgcat
    15181 aaacggcatc tggaaggttt atttactgcc ggaaaggtga tggtgattga acggcgcaac
    15241 ttccagcgcg tttatgattt aacccaccgt gtcatgcctg actgggatga tgagcgcgat
    15301 ctcgtttcgc aaacagaagc agaaatcatc atgctggata acagtgcgcg tagccaggga
    15361 atattccgcg aacagtggct ggcagattac tatcggctga aacgtccggc actggcagcg
    15421 tggcgcgaag cgagggctga acagcggcaa atcattgctg tgcatgttga aaaattgggc
    15481 aatctttggc tgcatgctga tttgctgccg ctactcgagc gagcgctggc cggaaagctc
    15541 actgcaacgc acagcgcggt actttcgcct tttgatcctg ttgtctggga tcgcaaacgc
    15601 gcagagcagc tttttgattt tagctaccgg ctggagtgct ataccccagc gccgaaacgc
    15661 cagtatggct attttgttct gccgttatta catcgtgggc aattagttgg gcgaatggat
    15721 gccaaaatgc atcgccagac aggcatcctt gaagttatct ctctgtggtt acaggaaggt
    15781 attaaaccaa cgacaacgct gcaaaaaggg ttacgtcagg cgattactga tttcgctaac
    15841 tggcagcagg caacgcgggt gacattagga cactgcccgc aaggtctctt tacggattgc
    15901 cgcaccggct gggaaataga ccccgtcgca taaatgatta tgataagatt tatgcattca
    15961 taacccgatc catctggagg aactatggat catcgtctgc ttgaaatcat tgcctgcccg
    16021 gtttgcaacg gaaaactttg gtataaccag gaaaaacaag aacttatttg caaactggac
    16081 aacctggctt tcccgctgcg cgatggcatt ccagtgttgc tggaaacgga agcccgcgtg
    16141 ctgactgctg atgagagtaa atcatgagtt ttgtggtcat tattcccgcg cgctacgcgt
    16201 ccacgcgtct gcccggtaaa ccattggttg atattaacgg caaacccatg attgttcatg
    16261 ttcttgaacg cgcgcgcgaa tcaggtgccg agcgcatcat cgtggcaacc gatcatgagg
    16321 atgtcgcccg cgccgttgaa gccgctggcg gtgaagtatg tatgacgcgc gccgatcacc
    16381 agtcagggac agaacgcttg gcggaagttg tcgaaaaatg cgcgttcagc gacgacacgg
    16441 tgatcgttaa tgtgcagggt gatgaaccga tgatccctgc gacaatcatt cgtcaggttg
    16501 ctgataacct cgctcagcgt caggtgggta tggcgactct ggcggtgcca atccacaatg
    16561 cggaagaagc gtttaacccg aatgcggtga aagtggttct cgacgctgaa gggtatgcac
    16621 tgtacttctc acgcgctacc attccgtggg atcgtgatcg ttttgcaaaa ggccttgaaa
    16681 ccgttggcga taacttcctg cgtcatcttg gtatttatgg ctaccgtgca ggctttatcc
    16741 gtcgttacgt cacctggcag ccaagcccgt tagaacacat cgaaatgtta gagcagcttc
    16801 gtgttttgtg gtacggcgaa aaaatccatg ttgctgtcgc gcatgaagtt cctggcacag
    16861 gtgtggatac ccctgaagat ctcgagcgcg ttcgcgctga aatgcgctaa tttcacttca
    16921 cgacacttca gccaattttg ggaggagtgt cgtaccgtta cgattttcct caatttttct
    16981 tttcaacaat tgatctcatt caggtgacat cttttatatt ggcgctcatt atgaaagcag
    17041 tagcttttat gagggtaatc tgaatggaac agctgcgtgc cgaattaagc catttactgg
    17101 gcgaaaaact cagtcgtatt gagtgcgtca atgaaaaagc ggatacggcg ttgtgggctt
    17161 tgtatgacag ccagggaaac ccaatgccgc taatggcaag aagctttagt acgcccggaa
    17221 aagcccgaca actggcatgg aaaaccacca tgctggcaag aagtgggact gtccgtatgc
    17281 cgactattta tggtgtgatg acgcatgaag aacaccccgg ccctgatgtc ctgctactgg
    17341 agcggatgcg tggtgtttcg gtagaggcac cagcccgaac accagaacgt tgggaacagc
    17401 tcaaagacca aatcgttgaa gccttactgg cctggcaccg tcaggacagt cgcggttgcg
    17461 tcggcgcggt cgacaatact caggaaaatt tctggccctc atggtaccgg caacatgttg
    17521 aagtgctatg gaccacgctc aatcagttca ataacaccgg tctgacgatg caggataagc
    17581 ggatcctgtt tcgcactcgc gaatgtctcc cggcattatt tgaaggtttt aacgacaatt
    17641 gtgtgctgat tcacggtaac ttctgtttac gcagcatgtt gaaagattcg cgcagcgatc
    17701 agttactggc gatggtcggg ccgggactaa tgctttgggc accgcgagaa tacgaactgt
    17761 tccgactaat ggataattct ctggcggaag atttgctctg gagttacctg caacgcgcgc
    17821 cagtggcgga gtcgttcatc tggcggcgtt ggttgtatgt gttatgggat gaagttgcgc
    17881 aactggttaa taccggacga tttagtcggc gcaacttcga tctggcatca aaatcactct
    17941 tgccgtggct cgcctgacga accttttagc cactgccaga tgcggccaag cgtttcatag
    18001 ccaacacgat cgctatgcat caaccagacc ggagaaggga ttgcccgttc ccacgggtta
    18061 agcggcgagt cgatggccaa ctgattcgcc ggggctggta gtggatttaa cccttcctgc
    18121 tgaaaaaaga tcatcgcccg cggcagatgt gatgcggagg tcaccagcag gaaaggggcg
    18181 tcaccaatcg cctgtttcac tgccgcagct tcttcttcgg tatcttttgg caaatccagg
    18241 gtgataattt gctcgcgcgg tacgcccagc gattgcgcaa ctctggcacc tacttccgct
    18301 gtactcaccg tattggtttt tgctacgcct cccgtgaaga tcagttttga acccggattt
    18361 tcgcgccata aacggatacc ctcattcagg cgtggcagac tgttattgat taaattagag
    18421 ctcggtgccc actgcgggtt ccaggtataa ccacctccga gcaccacgat atagtccact
    18481 ttttgcgaat tattccatgt cggataggtg ctttcgatgg gacgcagcag gcggtcggct
    18541 accggttgca aactcaataa caagagtgcc agccacccaa tgctaataaa aatcttcccg
    18601 gttttttgaa aacggctaaa ccacagtagc gccaggccag cgccaataat taacaacatc
    18661 agcggaagtg gcagcaacat attgccaatc acttttttca gtgtaaaaag catcctttct
    18721 ggttcctttt ttaaccatat agcaagggat cgccagcgat attacaccag acgggttcat
    18781 tctcgccgtg gctgtgacaa aatagcgtct ttatcagaaa acgtcttagc caaccggtgg
    18841 agaaagcaat gcaggatcgc aattttgatg atattgcgga aaagttttcc cgtaacattt
    18901 acggcaccac caaagggcag cttcgacagg ctattctgtg gcaggatctc gatcgcgtgc
    18961 tggcggaaat gggtccgcaa aaactgcgtg tgctggatgc gggcggtgga gaagggcaga
    19021 ccgcaatcaa aatggccgag cgcgggcatc aggtcatttt atgcgatctt tctgcgcaga
    19081 tgatcgaccg cgcaaaacag gcggcagaag caaaaggtgt gagcgacaac atgcaattta
    19141 tacattgcgc cgctcaggat gttgcttcgc atttggaaac gcccgttgat ctgatattgt
    19201 tccatgcggt gctcgagtgg gtggctgatc cccgcagcgt attgcagacc ctgtggtcag
    19261 tgttgcgtcc aggcggcgtg ttgtcgttaa tgttctacaa tgcgcatggt ttgttgatgc
    19321 ataacatggt cgccgggaat tttgattacg tgcaggcggg aatgccgaaa aagaaaaaac
    19381 ggacgctttc gccagattat ccacgcgacc cgacgcaggt ttatctgtgg ctggaagaag
    19441 ctggttggca aattatgggt aagacaggcg ttcgcgtgtt tcatgattat ctgcgcgaga
    19501 agcaccagca gcgcgactgc tatgaagcat tacttgaatt agaaacgcgt tattgccgtc
    19561 aggaaccgta tattaccctg gggcgttata ttcatgtcac cgcgcgcaaa ccgcagagca
    19621 aggataaagt atgagtgaat tttcccagac agtccccgaa ctggttgcct gggccagaaa
    19681 aaatgacttc tccatttcgc tgccggtaga ccgactctct tttctgctgg cagttgccac
    19741 gctgaacggc gagcgtctgg atggtgagat gagtgaaggc gagctggtgg atgcattccg
    19801 ccatgtgagt gatgcgtttg agcaaaccag cgaaaccatc ggcgtgcgcg ccaacaacgc
    19861 gatcaacgac atggtgcgtc aacgtctgct gaaccgcttt accagcgagc aggcagaagg
    19921 gaacgcaatt taccgtctga cgccgctcgg catcggcatt actgactatt acatccgtca
    19981 gcgcgagttt tctacgctgc gcctttctat gcagttgtcg attgtggcgg gtgagctcaa
    20041 acgcgcagca gatgccgccg aagagggcgg tgatgaattt cactggcacc gtaatgtcta
    20101 tgcgccactg aaatattcgg tagcagaaat tttcgacagt atcgacctga cgcaacgtct
    20161 gatggacgaa cagcagcagc aggtgaagga cgatatcgcc cagttgctga acaaagactg
    20221 gcgggcggcg atttccagct gtgaattgtt gctttcggaa acttccggaa cgctgcgtga
    20281 attgcaggat acactggaag cggcaggcga caaattgcag gctaatctgt tgcgcattca
    20341 ggatgcgacg atgactcatg acgatctgca tttcgtcgat cgtctggtgt tcgatctgca
    20401 gagcaaactc gaccgtatta tcagttgggg ccagcaatcc atcgatttgt ggattggcta
    20461 cgaccgccac gtacataaat ttattcgtac cgcgatcgat atggataaaa accgcgtctt
    20521 tgctcagcgg ttacgtcagt cggtacaaac ctattttgat gagccgtggg cgctaagtta
    20581 tgccaatgcc gatcgtctgc tggatatgcg tgacgaagag atggcactgc gcgatgaaga
    20641 agtgactggg gaacttccgc cagatttgga atacgaagag tttaacgaga tccgcgaaca
    20701 gctggcggcg atcatcgaag aacaacttgc cgtgtacaaa accagacaag tgccgctgga
    20761 tcttggtctg gtggtacgcg aatatctgtc acagtatccg cgtgcacgtc actttgacgt
    20821 tgcgcgcatt gttattgatc aggcggtacg tcttggcgta gcgcaagcag atttcaccgg
    20881 actgccagcg aaatggcagc cgattaatga ttacggagcc aaggtacagg cgcatgtcat
    20941 cgacaaatat tgaacaagtg atgccggtta agctggcgca ggcgctggcg aatccgttat
    21001 ttccggcgct ggacagcgcc ttacgttcag gccgccatat tggcctcgac gaactggata
    21061 atcatgcatt cctgatggat tttcaggaat atctggaaga gttttacgcg cgttataacg
    21121 ttgagcttat tcgtgcaccg gaagggttct tctatttacg tccacgttcc accacgctga
    21181 tccctcgttc cgtcttgtcg gaactggata tgatggtcgg gaaaatcctc tgttatctct
    21241 atctcagccc ggaacggctg gcgaatgagg ggattttcac ccagcaggaa ctgtacgacg
    21301 aactgctcac cctggccgat gaagcgaaac tgctgaaact ggtgaacaac cgttcaaccg
    21361 gttcagacat tgaccgtcag aagttgcagg aaaaagtacg ttcttcgctc aaccgtctgc
    21421 gtcgtttagg tatggtgtgg tttatgggcc acgacagcag caagttccgc attaccgaat
    21481 cggtgttccg cttcggagcc gatgtgcgtg ctggcgacga tccccgtgaa gcacagcgtc
    21541 gcctgattcg tgatggcgaa gcaatgccga ttgaaaatca tctgcaactc aacgatgaaa
    21601 ccgaagagaa tcagccagat agcggagagg aagaataatg attgaacgcg gtaaatttcg
    21661 ctcactgacg ctgattaact ggaacggctt ctttgcccga acttttgacc ttgacgagct
    21721 ggtcacgacg ctttctggcg gtaacggggc gggtaaatcc accaccatgg cggcgttcgt
    21781 tacggcgctg atccccgacc tgaccctgct gcatttccgt aacactacgg aagccggggc
    21841 caccagcggt tcgcgcgata aaggtctgca cggtaagctg aaagcgggtg tctgttattc
    21901 gatgctcgac accattaact cgcgccacca gcgcgtggtg gtcggtgtgc gtctgcaaca
    21961 ggttgccgga cgcgatcgta aagtggatat caaaccattt gccattcagg gactgccgat
    22021 gtcggtgcag ccgacacagc tggtgaccga aactctgaac gaacgccagg cgcgcgtgct
    22081 gccgcttaac gagctgaaag acaagctcga ggcgatggaa ggcgtgcagt ttaaacagtt
    22141 caactccatt accgattacc actcgctgat gttcgatctg ggcatcattg cgcgtcgtct
    22201 gcgctccgca tctgaccgta gcaaattcta tcgtctgatc gaagcttcgc tgtatggcgg
    22261 gatctccagt gccattaccc gttctctgcg cgactacctg ttgccggaaa acagcggcgt
    22321 gcgtaaagcg ttccaggaca tggaagcggc gctgcgtgaa aaccgcatga cgctggaagc
    22381 gattcgtgtc acccagtcgg accgtgacct gtttaagcat ctgatcagcg aagctaccaa
    22441 ctacgtggcg gcggactaca tgcgtcacgc caacgagcgc cgtgtccatc tcgataaggc
    22501 cctggagttt cgtcgcgaac tacatacttc gcgtcagcaa ctggcggcgg agcagtacaa
    22561 acacgtcgat atggcgcgtg agctggcaga gcacaacggt gccgaaggcg atctggaagc
    22621 ggattatcag gcggccagcg atcacctgaa cctggtgcaa accgcactgc gtcagcagga
    22681 gaagatcgaa cgctacgaag cggatctcga tgagctgcag atccgtctgg aagagcaaaa
    22741 cgaagtggtg gcagaagcca tcgaacgcca ggaagagaac gaggctcgcg cggaagctgc
    22801 cgaactggaa gtggacgagc tgaaaagcca gcttgctgac taccagcagg cgctggacgt
    22861 ccagcaaacg cgcgcgatcc agtacaacca ggcgattgct gcgcttaatc gtgccaaaga
    22921 actgtgccat ctgccggact taaccgccga cagcgccgcc gaatggctgg aaaccttcca
    22981 ggcgaaagag ctggaagcga ctgaaaaaat gctctctctt gagcagaaaa tgagcatggc
    23041 gcaaaccgcg cacagccagt ttgagcaggc ttatcagctg gtggtggcaa tcaacggccc
    23101 gctggcgcgt aacgaggcgt gggatgtcgc tcgcgaacta ttgcgcgaag gggtcgatca
    23161 gcgtcacctg gcagagcagg ttcagccgtt gcggatgcgc ttaagcgaac tggaacagcg
    23221 tctgcgcgaa cagcaagaag ctgagcgtct gctggcagat ttctgcaaac gtcagggcaa
    23281 gaattttgac attgacgagc tggaagccct gcatcaggag ctggaagcac gcattgcctc
    23341 tctttccgat agcgtgtcta acgcccgtga agagcggatg gcactgcgcc aggagcagga
    23401 acagctgcag tctcgcattc agagtttgat gcagcgtgca ccggtttggc tggcagcgca
    23461 aaacagtctc aaccagttga gcgaacagtg cggcgaagag ttcacctcca gccaggacgt
    23521 cactgaatat ctgcaacagt tgctggagcg tgagcgcgag gccattgttg aacgcgatga
    23581 agtaggcgcg cgcaaaaacg ccgtcgatga agagatcgaa cgtttaagcc agcctggcgg
    23641 ctctgaagat cagcgtttga acgcgctagc ggagcgtttt ggtggtgtgc tgctgtcaga
    23701 aatttatgac gacgttagcc tggaagatgc gccgtacttc tcagcgctgt atggcccgtc
    23761 acgccacgcc atcgtggtgc cagatctgtc acaggtaacc gaacacctgg aaggtttgac
    23821 cgattgcccg gaagatctct atttgatcga aggggatccg cagtcattcg atgacagcgt
    23881 gttcagcgtt gatgagctgg aaaaagcggt agtggtgaaa atcgccgatc gtcagtggcg
    23941 ttattcacgt ttcccggaag tgccgctgtt tggtcgtgct gcgcgtgaaa gccgtattga
    24001 aagcctccat gccgagcgtg aagtgctttc cgaacgcttc gccacgctct cctttgatgt
    24061 acagaaaacc cagcgtctgc atcaggcgtt cagccgcttt atcggcagtc atctggcggt
    24121 ggcgtttgag tctgacccgg aagcagaaat ccgtcaactg aacagccgtc gcgtcgaact
    24181 ggagcgggcg ttgagtaatc atgaaaatga taaccagcag cagcgtattc agtttgagca
    24241 ggcgaaagaa ggcgttacgg cgctgaaccg cattctgccg cgtctcaacc tgttggctga
    24301 tgacagcctg gcggatcgcg tcgatgaaat ccgcgaacgt ctggatgaag cccaggaagc
    24361 cgcgcgtttt gttcagcagt ttggcaatca actggcgaaa ctggaaccga tcgtttcggt
    24421 attgcagagc gacccggaac agttcgaaca gttaaaagaa gattacgcgt actctcagca
    24481 gatgcagcgc gatgcccgtc agcaggcgtt tgccctgacg gaagtggtgc agcgtcgtgc
    24541 gcactttagc tactctgact cggcagaaat gcttagcggt aacagcgatc tcaacgaaaa
    24601 actgcgtgaa cgtctggaac aggcggaagc ggagcgtacc cgcgctcgcg aagcgttgcg
    24661 cggtcacgca gcgcagttga gtcagtacaa ccaggtgctg gcttcgctga aaagttctta
    24721 cgacaccaaa aaagagctac tcaacgatct gcaacgtgaa ttgcaggata tcggcgtgcg
    24781 tgctgatagc ggggcagaag agcgggcacg tattcgccgt gacgagctgc atgcacaact
    24841 gagcaataac cgttcgcgcc gcaatcaact ggaaaaagcg ctcaccttct gcgaagcgga
    24901 gatggataac ctgacccgca aactgcgcaa gctggagcgt gattactttg agatgcgcga
    24961 gcaggtagtg accgcgaaag cgggctggtg tgcagtcatg cgcatggtga aagataacgg
    25021 cgttgagcgc cgcttacacc gtcgtgagct ggcttatctc tccgctgatg atttgcgttc
    25081 catgtcggat aaggcgttag gtgcgctgcg tctggcggtg gcggataacg aacatctgcg
    25141 cgacgtgctg cgcatgtcgg aagatcccaa acgtccggag cgtaaaattc agttcttcgt
    25201 ggcggtttat cagcatctgc gtgaacgtat tcgtcaggat attattcgta ccgatgatcc
    25261 ggtggaagct atcgaacaga tggagatcga acttagccgt ctgaccgaag agttaacctc
    25321 ccgtgaacag aaactggcga tcagttcccg cagcgtggcg aacatcattc gcaaaaccat
    25381 tcagcgcgag cagaaccgta tccgtatgct caaccagggg ttgcagaacg tatcgtttgg
    25441 tcaggtgaac agcgtgcgcc tcaacgtgaa cgtgcgtgaa acgcacgcca tgctgctgga
    25501 tgtgctctct gaacagcatg agcagcatca ggatctgttt aacagcaacc gtctgacctt
    25561 ctcggaagcg ctggcgaaat tgtatcagcg tcttaatccg cagattgata tggggcagcg
    25621 cacgccgcag accattggcg aagaactgct ggattaccgc aactatctgg aaatggaagt
    25681 tgaggttaac cgtggttccg atggctggtt gcgcgcagag tctggtgcat tgtcgaccgg
    25741 tgaggcgatt ggtaccggta tgtcgattct ggtgatggtg gtacaaagct gggaagatga
    25801 atctcgccgc ctgcgcggta aagatatctc tccttgccgc ctgctgttcc tcgatgaagc
    25861 agcgcgactg gatgctcgtt ctatcgccac gctgtttgaa ttgtgtgagc gtttgcaaat
    25921 gcagctcatc atcgcagcgc cggaaaatat cagcccggag aaaggcacca cctataaact
    25981 ggtgcgtaaa gtcttccaga ataccgaaca tgttcatgtc gtcggcctgc gaggatttgc
    26041 gccgcaactc cctgaaacgc ttccaggaag tgacgaagcg ccttctcagg cgagttaaaa
    26101 ttaaggcggc agcaatgccg ccttttcttt ttccgaaaac tccgtttctg cactaaaaaa
    26161 gtggcacatt acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta
    26221 tcggcgttta tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg
    26281 tgagtattgg cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat
    26341 aaaaacaggg ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc
    26401 agtttgtgcc tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa
    26461 gtaatccctg gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg
    26521 caagccacgg caataatggc ggggatcctg ccattgcctg aaggtgcggc agaaaaagcc
    26581 cgcacgcaaa tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt
    26641 caactgttgt atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa
    26701 gccttccagc aacagctggc agaggtggcg attgccggtt tccagccgca gtttaataaa
    26761 tgggtagagt tactgaccga tcctggtgtt aacgggatgg cacgcgacgt ggtgctctct
    26821 gatgcgatga tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg
    26881 ctatatagca gtaaacctta tgcgcttgca acgccgccgc tttcggtgat taaccaatgg
    26941 cagcaggcgc tggataaagg tcaattgcct acgtttgttg caggactggc accgcagcat
    27001 ccgcaatatg cggtgatgca tgaatcgtta ctggccttac tcagtgacac caaaccgtgg
    27061 ccccaactga ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg
    27121 ttgcgtgaaa tattgcaacg cacaggcatg ttggacgggg ggccgaaaat tactctacct
    27181 ggcgatgaca cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca
    27241 gaaactaagc cgatggataa gcaaacgacg tctcgtagta aacctgcgcc tgccgttcgc
    27301 gccgcctacg ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg
    27361 ggggcagatg gtgctattgg cccggcaacg cgtgactggt taaacgtaac gcccgcccag
    27421 cgtgctggcg tgttggctct caacatccag cgattgcgct tgctgccaac agagctttct
    27481 accgggatca tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag
    27541 gtgctggatt cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt
    27601 gcccttaaca atgtagtggt aaacccgccg tggaacgtac ctccaactct ggcacgcaaa
    27661 gatattctgc cgaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg
    27721 atgcgcggct ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc
    27781 acggcctcga atttaccgtt tcgcttccaa caggctccag gcccacggaa ctcgctgggg
    27841 cgctataaat tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac
    27901 aatctgttca agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct
    27961 tccgatctgg cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat
    28021 gcgctgaagc agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc
    28081 tactacctga cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac
    28141 aattatgatc tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc
    28201 aggtaaatga agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggcaa
    28261 ttctctgcag cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg
    28321 ttaaggtgcc tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg
    28381 attatcatgg acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca
    28441 ctcggtgccg ccatcctgcc gacccctgcg tttgcaacac tctctacccc acgcccgcgc
    28501 attttgacac tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc
    28561 agaggctata ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg
    28621 aacaaaataa agtccatcga cccaggatta ttcgaccagt tgtatcgcct gcaagggtta
    28681 ttaggcaccc gcaaaccggt gcaactcatt tccggttatc gttccattga taccaacaat
    28741 gaactacgcg cccgcagccg tggagtagcg aagaaaagct atcacactaa aggccaggcg
    28801 atggatttcc atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg
    28861 cgcgcaggtg gtgtaggata ttacccacgt agtaactttg tgcatattga taccgggcca
    28921 gcacggcact ggtagtaatt gcttaacgaa acaggggcag tatgaactat cgtattattc
    28981 cggtcaccgc attctcccag aactgttcat tgatctggtg tgaacaaacc cgcctggccg
    29041 cactggtcga tcctggcggc gatgcggaaa aaatcaaaca ggaagttgac gccagcggcc
    29101 tgacactgat gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg
    29161 aactggcgca acattacggc gtgccggttt ttggcccgga aaaagaagat gagttctggc
    29221 tgcaaggctt gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag ccgctgacgc
    29281 cagatcgttg gctgaacgaa ggcgatacca tcagcatagg gaatgtgact ttacaggtgt
    29341 tacattgccc tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc
    29401 tgatttctgg cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg
    29461 atcataatca actgatttct tcaatcaaag ataaattgct gccactgggt gatgacgtga
    29521 catttattcc gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct
    29581 tcctgcaaga cgaaatgccc gtctggtaag gcacacataa aaaagcccgc ttttcagcgg
    29641 gcttcattgt ttttaacgct tacagcactg ccacaatcgc ttcgcacagc ggagccatgt
    29701 tatctggtgt catcccggcc acgtttacgc gaccagaagc aacagcatac acgccaaact
    29761 cttcgcgcag acgcagcact tgttcttttg tcaggccact gaaggagaac atgccgttct
    29821 gtttgatgat aaagctgaag tcgcggtttg cgcctttttc ctgcagcgta ttgacgaaca
    29881 actgacgcat acgctgaata cgctggcgca tatcagtcag ctcttgttcc caaatcgcac
    29941 gtaacgcatc gttgctcagg atggtggcaa caacagaagc gccgtgtgct ggtgggttag
    30001 agtagttagc gcgaatcgcc gctttcattt ggctgaatgc gcgatcaacg gtttcactgt
    30061 cggcagcaac cagagtacaa gcgccaacac gctcgttgta caggccaaag tttttagagt
    30121 aggaactggc aacaatcagc tctttatgca tagccgcgaa agcgcgcagt ccttcagcat
    30181 cttcttccag accacgggca aaaccctggt aagcgaagtc aaacagcggt aaccagcctt
    30241 tctcaacgga gagttgtgcc agtgtctgcc attgttccag cgtagggtcg ataccggttg
    30301 ggttgtggca gcagccatgg aacagcacta cgtcgccagc ctgagcttcg ttcaggctgt
    30361 taatcagtgc atcgaagtca agggtgtggt tttccgcatc ataataagcg tattcacgaa
    30421 cttccagatc tgcagagtta aagacgctct tatggttcgg ccagcttggg ttgctcaccc
    30481 acactcgctt aacgctggta ttttttgcca ggaaatcggc agctatgcgt agtgcgccag
    30541 tgccacccgg agtctgtgcc gtgcgagcac gtttgtcatt gatcagggcg ctacctttac
    30601 caaacagcag ttcctgagtg cagcgaccaa attcagggat gccgtcaatg ccgaggtaat
    30661 ttttggtggt ttcattttcg agcagatact gttcagcctt tttcacgctg gtcagtaccg
    30721 gggttttgcc cgtctcatct ttatagacac caatcccgag gttaattttg ccgggacgtt
    30781 catcggcacg aaacagatcg gccaggccca gaatcgggtc ggcaggagcg gcggtaatgt
    30841 tctcaaacat gacgaggttc cattatggtt acagaaggga agtccgctat cagggtaacg
    30901 ggagatttac aaaattccaa ctattactga tgaaaacgca ggctgttttt gcaagacgtg
    30961 agattgctct ggaaggcata aaaaaaacag gaccaaagtc ctgttttttc ggcatttaac
    31021 aaagaggtgt gctattagaa ctggtaaacg atacccacag caacggtgtc gtctgaacct
    31081 acgcccagtt tgttgtcaga atcgatctgg ttgatgatgt agtcaacata ggtggacatg
    31141 tttttgttga agtagtaggt tgcgcccact tcaaagtagt tcaccagatc aacatcaccg
    31201 ataccttcta cgtctttcgc tttagatttg gtgtaagcga tggacggacg cagaccgaaa
    31261 tcgaactggt attgcgcaac taacagaacg tcttgcgttt tgttggcgaa gccgctgatg
    31321 tttgtaaatt tattagtgat cggcgtagcg ttacgcgttt caccgtagtt cgctgccagg
    31381 tagatgttgt tcgcatcata cttcagacca gtagcccact gttcagcttt tttaccgttg
    31441 ccaagaagtt gagcttcttg caggttggta cggtcagctg caccataagc accaacgata
    31501 ccaaagcctt cgtattcgta gctgatagaa ccgccaacac cgtcgccgtt agagcggcgt
    31561 gcagtgtcac gctcgttttt acccaggtac tgaacagcga agttcaggcc atcaaccaga
    31621 ccaaagaagt tggagttacg ataggtagca acgccgccaa cacgaccaac gaagaagtca
    31681 tcgctgtatg cagtatcacc accaaattct ggcagcatat cggtgtaacc cagtgcatca
    31741 taaaccacac cgtagttacg gccgtaatcg aaagaaccaa cgtcagcgta tttaagaccc
    31801 gcgaatgcca gacgcgtttt gttaccagtt tgagcgtcag cgccttcaga gttgttaccc
    31861 tggaagttat attcccactg accataaccg gtcagatcgg aattgatttg agtttcccct
    31921 ttaaaaccaa gacgggcata ggtcatgtcg ccattgccac cgtaactgtt ttcaccgtta
    31981 cccttggaga aataatgcag accgacagct ttaccgtaca gatctacttt gttgccatct
    32041 ttgttataga tttctgcagc gtttgcagta cctgctacta acagagcagg gacgatcact
    32101 gccagaatat tgcgcttcat cattatttat taccctcatg gtttttttta tgacacctgc
    32161 cactgccgtc aataatttct gtcaataaaa ttttacggaa ctattgatga gagtttggtg
    32221 tctttatgtg tctgcaggca tctttccatt caaactaacg tttcgctacc gtgaaagtgc
    32281 tacaaagata aagatttagt ttcaaaaaga aaaaatatgt aactaaaagt aaaaattaag
    32341 gaactttgtg aacaccgtca tatttccata gaggcgtgat gatatttaca gcaattttta
    32401 tctatttata tgatttcctt atatttaaat taactaaacg gaaattttgt ttctgatgga
    32461 aactttatcg acctggccca aaatcctctt tcagacatcc agaatgccaa aaaaatagta
    32521 tgaaaattgt gctatcagct aagaaaaaac taatccgcaa taagaatgcg ggttattttt
    32581 tgtgcgtaaa tgttgcaatg taatattccg taacaggatg atcgttatgg cggggttgca
    32641 aatagattga ttgacttaac aaacagataa cttgacagaa aggataaaaa agagccagcg
    32701 ggtgctggct cttgtagacg ctaaattaga agctggcgtt acgcggagtt cgtgggaacg
    32761 gaatcacatc acgcacgttt tgcacaccag ttacgtaagc aatcaggcgt tcaaaaccaa
    32821 gaccgaaacc ggaatgcgga acagtaccgt agcgacgcag atcgcgatac caccagtaat
    32881 cttctttgtt caggcccatt tccagcatac gctcgtccag cacgtccaga cgctcttcac
    32941 gctgggagcc accaatgatc tcaccgatgc ccggagccag aacatccata gccgcaacgg
    33001 ttttaccgtc ttcgttgagg cgcatataga acgctttaat atctttcgga tagtttttaa
    33061 ccactaccgg tgctttaaag tgttcttccg ccagataacg ctcatgctca gaagagagat
    33121 cgacgcccca gtaaaccggg ttttcaaact tcctgccgca gttttcgaga atggttactg
    33181 cttcggtgta atccacctgc gcgaaatcgg cctcaataaa gcgttccaga cgtgaaacgg
    33241 catctttatc tacgcgttca gcgaagaatt tcatgtcgtc agcgcgttct tcgagaaccg
    33301 ctttgaagac atatttcagc atggcttcag ccagacccgc gatatcgttc aggttagcaa
    33361 acgccacttc cggctccagc atccagaatt ccgccaggtg acggctggtg ttggagtttt
    33421 cagcacggaa agtcgggccg aaggtataaa ttttggataa tgcgcaagcg taggtttcgc
    33481 cgttcaactg gccagatacg gtcaggaaag actctttacc aaagaagtct ttgtcgaaat
    33541 ccactttgcc ctgatcgtta cgcggcagat tttccagatc cagcgtagaa acgcggaaca
    33601 tttcgccagc cccttcggta tcagacgcag taatcagtgg cgttgaaacc cagaagaatc
    33661 cctgctcgtt aaagaagcga tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg
    33721 caccaatcag gtttgtgcgc ggacgcaggt gagcgacttc acgcagatac tcaatgctgt
    33781 ggcgttttgc cgccatcggg taagtgtctg gatcttcaac ccaaccagca acttcaacct
    33841 tgctggtctg aatttcaaat tgttgcccct ggcccggcga cgccacgact ttacccgtca
    33901 caatgaccga gcagccggtg gtcagacgca ggacgtcttc attgtaattg ggcagagaat
    33961 tattgatgac agcctgtaca ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga
    34021 tgccagcttt tgaatctcgg cgggtacgta cccatccgcg tacggtgact tcgctgtcaa
    34081 cggctacgcg gccctggagt acgtcggcta caggcacaac gctcataata ttctctctgt
    34141 taatagtcgg aaaaaataaa cacttgtcca cccgtaaatg gggggatacc tatgttacct
    34201 ggcatctgca atcagacaag cagaattcgc aaatgcagcg aaagatttcg gaattaaagg
    34261 taaaaaaagg gagccgatta gctccctgat gatattaact ggctttttta atatgcggaa
    34321 ggtcgaacgc tttgcgcagc gcccgaacaa acgctttatc atggcagata gttttgccag
    34381 ggctgtcaga aagtttcgcc accggtttac cgttacactc taccaactta atcacaatat
    34441 tcaggggttt tacctggggg atatcgcagg tcaggcgagt cccaatacca aaacttaatt
    34501 gcacgcggga agagaagtgg cggtatagct caaccgcttt gcgtaaatcc agattgtcag
    34561 agaaaaccag cgttttactc tgtggatcaa tttccagctt ttcataatgt gcaatggctt
    34621 tttcacccca ttcaaccggg tcgccagagt catgacgcag gccctgatac cgactagcga
    34681 actcgacacc gaaatcacgc aggaaagcat ccatagtgat gcagtcggtt aatgcaatgc
    34741 caagttggtc gggatactct tccagccagg cagcaagtgc agctcgctgg ctgttggcta
    34801 gatccgggct gatttgctga tgtgcctgga accattcgtg tgcctgtgtt cccatcggcg
    34861 tgagggaaag ccgacgcgcc agatcgtagt tgctggtgcc cacgaaccag gattcctgtt
    34921 gcagacgctt aacgatggtt tcttgtactt cgcgagaaaa acggcgacgg gtgccaaaat
    34981 ccatcagatg gaagcgcgac atatcaagac cagcggttaa cgccgagaag tcgactaatt
    35041 tgctttccag cgtgtcgagg gcttgcgcaa cgtcggcctg cggtgagcga tagcgatgta
    35101 ccatttcact gataaccgcc agcaaaggaa cctcccagag gatgacttca cgccacgggc
    35161 cgcttaaacg aatatccagc ttgccattat cgttggacac ggtgacttgt tccgggttaa
    35221 agcggaactc gcgtaaccag ttaagatagt cggccttaaa gaaaggcagg gcagaaagcc
    35281 actgatattc atcatcctgc aggcgcaggt gctgcatcgc ctgaacctgt tcacgaatag
    35341 catcggcata aatacccagc agatcgtcac ctcggcaacg aaactccgcc gcgacatgca
    35401 catcgtaata atgatgaaac acggcttgct gcatatgcaa cttataagca tctgtatcca
    35461 gcaacgagtg cagaacagga gaagcgaatt gtgtcatagg tgcgcagtag cgtcctcgta
    35521 caggagcgtt tagtacaata aacatcttca ggaactgctg gagtatacct tgtttcgcaa
    35581 tttattgaac cccgatcaca ccatatgcca cctttctggt cgatggcatt acgcggtgta
    35641 tgttataaaa atgtagcaat aaaggcgttt gtacctgaaa agatgaagat tctgcatagc
    35701 gcgatttacg caacaggaat agactgaaca ccagactcta taaaagatgc taaaggttat
    35761 ttatgactca acagccacaa gccaaatacc gtcacgatta tcgtgcgccg gattaccaga
    35821 ttactgatat tgacttgacc tttgacctcg acgcgcaaaa gacggtcgtt accgcggtca
    35881 gccaggctgt ccgtcatggt gcatcagatg ctccgctttg tctcaacggc gaagacctca
    35941 aactggtttc tgttcatatt aatgatgagc cgtggaccgc ctggaaagaa gaagagggcg
    36001 cactggtcat cagtaatttg ccggagcgtt ttacgcttaa gatcattaat gaaataagcc
    36061 cggcggcgaa tacggcgctg gaagggcttt atcagtcagg cgatgcgctt tgcacccagt
    36121 gtgaagccga aggtttccgc catattacgt attatctcga ccgcccggac gtgctggcgc
    36181 gttttaccac caaaattatt gccgataaaa ccaaatatcc cttcctgctt tccaacggta
    36241 accgcgttgc gcaaggcgaa ctggaaaacg gacgccattg ggtacagtgg caggacccgt
    36301 tcccgaaacc gtgctacctg tttgcgctgg tggcaggcga ctttgatgta ctgcgcgata
    36361 cctttaccac gcgttctggt cgcgaagtag cactggagct gtacgtcgat cgcggcaacc
    36421 ttgatcgcgc gccgtgggcg atgacctcgc tgaaaaactc catgaaatgg gatgaagaac
    36481 gctttggcct ggagtatgac ctcgacatct atatgatcgt cgcggtggat ttcttcaata
    36541 tgggcgcaat ggagaataag ggtctgaata tctttaactc caaatatgtg ctggcccgca
    36601 ccgataccgc caccgacaaa gattacctcg atattgaacg cgttatcggc catgaatatt
    36661 tccataactg gaccggtaac cgagtgacct gccgcgactg gttccagctc agcctgaaag
    36721 aaggtttaac cgtcttccgc gatcaggaat tcagctctga ccttggttcc cgcgcggtaa
    36781 accgtatcaa taatgtgcgc accatgcgcg gattgcagtt tgcggaagac gccagcccga
    36841 tggcgcaccc aatccgcccg gacatggtca ttgagatgaa caatttctac accctgaccg
    36901 tttacgagaa gggcgcggaa gtgattcgta tgatccacac tctgcttggc gaagaaaact
    36961 tccagaaagg gatgcagctc tatttcgagc gccatgatgg cagtgcagcg acctgcgacg
    37021 atttcgttca ggcgatggaa gatgcgtcga atgtcgatct ctcccatttc cgccgttggt
    37081 acagccagtc cggtacgccg gttgtgaccg tcaaagacga ctacaatccg gaaaccgagc
    37141 agtacaccct gaccatcagc cagcgcacgc cagccacgcc ggatcaagca gaaaaacagc
    37201 cgctgcatat tccgtttgcc atcgaattgt atgacaacga aggcaaagtg atcccgttgc
    37261 agaaaggcgg tcatccggtg aattccgtgc tgaacgtcac ccaggcggaa cagacctttg
    37321 tttttgataa tgtctacttc cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag
    37381 tgaaactgga atataagtgg agcgatcagc aactgacctt cctgatgcgt catgcgcgta
    37441 atgatttttc ccgttgggat gcggcgcaaa gtttgctggc aacctacatc aagctgaacg
    37501 tcgcccgtca tcagcaaggg cagccgctgt ctctgccggt acatgtggct gacgctttcc
    37561 gcgcggtgct gctcgatgag aagattgatc cggcgctggc ggcagaaatc ctgacgctgc
    37621 cttctgtcaa tgaaatggct gaactgttcg atatcatcga cccgattgct attgccgaag
    37681 tacgcgaagc actcactcgt actctggcga ctgaactggc ggatgagcta ctggctattt
    37741 acaacgcgaa ttaccagagc gagtaccgtg ttgagcatga agatattgca aaacgcactc
    37801 tgcgtaatgc ctgcctgcgc ttccttgctt ttggtgaaac gcatctggct gacgtgctgg
    37861 tgagcaagca ataccatgaa gcaaacaata tgaccgatgc gctggcagcg ctttctgcgg
    37921 cggttgctgc acagctgcct tgccgtgacg cgctgatgca ggagtacgac gacaagtggc
    37981 atcaggatgg tctggtgatg gataaatggt ttatcctgca agccaccagc ccggcggcga
    38041 atgtgctgga gacggtgcgc ggcctgttgc agcatcgctc atttaccatg agcaacccga
    38101 accgcattcg ttcgttgatt ggtgcgtttg cgggcagcaa cccggcagcg ttccatgccg
    38161 aagatgggag cggttaccag ttcctggtgg aaatgcttac cgacctgaac agccgtaacc
    38221 cgcaggtagc ttcacgcctg attgaaccgc tgattcgcct gaaacgttat gatgccaaac
    38281 gtcaggagaa aatgcgcgcg gcgctggaac agttgaaagg gctggaaaat ctatctggcg
    38341 atctgtacga gaagatcacc aaagcactgg cttaataaat ccccgaatgg cggcaatagc
    38401 gccgccattc ggggatttta cccctgtttt ctcaggcgag tttcagattc accacgctgc
    38461 atcacccgtt gcaacacttc cgcttccagc tccgctaatc gcaccgaccc taaccggcgc
    38521 ggacggggaa tatccaccgt caaatccaga ccaatttttc cctcttcaat taacaacacc
    38581 cgatcagcca ttgctaccgc ttcgctcaca tcatgcgtca ccaacagtac ggtaaaaccg
    38641 tgctcctgcc aaagtgacac aatcaaatcc tgcatctcga gtcgcgttaa ggcatccagc
    38701 gcaccgagcg gttcatcaag caacaataaa cccggtcgat gaatcaacgc tcttgccagc
    38761 gccactcgct gtttctgccc gccagaaagt gcggcaggcc attccccggc gcgattctcc
    38821 agtcctaccg cagccaacgc ccgacgcgcg gcatcgcgcc actggccttt aaggcctaac
    38881 ccaacgttat caatcaccga tttccacggt agcagacgcg catcctgaaa catcattcgc
    38941 gtgtcttcct gaatttcagc cagcggtgtg gtgcccgcta acacatcgcc agcggttggc
    39001 gtttccagac ctgccagcag gcgcagcagg gtacttttgc caccaccgct gcggcccacc
    39061 accgccacaa actgacctgc cggaatatgt aaatccagtt ggttcaggac gatattttcc
    39121 gcgtaatgtt tgcttactgc attgagcaac aatggtgtgc cctggttcag acgagcagta
    39181 ttcataccgt ggcctccttc aaatgataag ccgggttcca gcgcaaccag aggcgctcta
    39241 acagctgcgc gctgacgtca gcaagtttgc cgagcagggc gtaaagaata atagcgacta
    39301 ccaccacgtc cgtttgcaaa aactcccgcg cattcatcgc cagataacca atgcctgaat
    39361 tggcagaaat ggtttcggca acaatcagcg tcagccacat caggcccaac gcaaaacgca
    39421 cgccgaccat aattgagggc agggcaccag gcaggatcac atggataaac agcggtatgc
    39481 cggataatcc atagctacgc gccatctcca ccaacccgcg atcgatatta cggatgccat
    39541 gccaggtgtt gatataaatg gggaatagcg taccgagcgc caccagaaag atttttgcgg
    39601 attcatcaat gccaaaccac aaaatcacca gcgggatcag cgccagatgc ggcacgttgc
    39661 gcaacatctg aattgaggta tccagcagcc tttctcccca gcgcgacagt ccgctaatca
    39721 gtcccaaaat caaccccagc gagccgccaa ttgaaaaacc aatcagcgcc cgccaggagc
    39781 tgatcgccag atgctgccac aattcgccgc tggctgagag cgtccagaac gccatcacca
    39841 ccccttccgg tgagggcaaa atacgcgtcg acaaccagcc aaccgaggag gccagttgcc
    39901 acaccgtcac gatgcccacc ggtaaaaacc agggggcaac gcgcaataac cacttcttca
    39961 ctggcgttgc cattatcttc ttccttagct ttgcgcgact ctgcgaggga taaaatcatt
    40021 ctccaccgct tcgccttgcg gattcagcgg ctgcggctgg ggaatttccg gaatggcgac
    40081 atccagatgt gggaacagca actcgccgac ccgatacgct tcttccagat gcggatagcc
    40141 cgaaagcaca aagctgtcga tgccaagcgc ggcatattcg ttgattcgcg cagcgaccgt
    40201 aggaccatcg cccaccagcg ccgtcccggc accgccgcgc actaagccaa cgcccgccca
    40261 taaattgggg ctgatctcca gattgtcgcg cttgccgtta tgtagcgctg ccattcgctg
    40321 ttgccccacg gaatccgtgc gggcgaatgc ggcttgtgct ttggcgatag tctcatcatc
    40381 aagatgcgag attaaccgct cggcggcctg ccacgcttcg tcgttagttt cacgaacaat
    40441 cacatgcaga cgaatgccga aacgaatttt gcgtccatgc gcggcagctt tcgcccgcac
    40501 ttgttcgatt ttctctttaa ccagttccgg cggttcgccc caggtgaggt agagatcaac
    40561 ctgttctgcc gccagctcct gggcgacgtc tgacgatccg ccaaagtaaa gcggaggata
    40621 cggctgttga atcggcggga agagcagttt ggctccccgc acatgaatat gtttaccgtt
    40681 gaaatcgacg gtttccccca gcaataaacg ccgccagacc tgggtaaatt ccgccgaggc
    40741 ttcgtagcgc tcactatgat caaggaacac gccgtcgcct gccagttctt gtggatcgct
    40801 gcctgtgacc aggttaaaca acgcacgtcc atttgagaga cggtcaagcg tggcggcctg
    40861 gcgggcggca acggtaggtg aggttacgct gggacgcagg gcgacaagaa acttcagccg
    40921 ctgcgtcacc gggatcatcg atgcggcaac cagccacgca tcttcgcagg agcgccccgt
    40981 tgggatcagc acaccggtat agccaagacg atccgccgct tgcgcaattt gttgcagata
    41041 accgtgatca accgggcgtg aaccttcttc cgttcccaga taatgcccgt caccatgggt
    41101 cggtaaaaac cagaacatat tcagactcat aattgttttc cttccagttg agtgggctgc
    41161 cagatgcgtt ggcgaatatc gacttttttc ggcaccagac gattttcata aaacagatct
    41221 gccgtttgct gctgtaaggc ggcaacctcg gcgttaaccg gtttgatggt ggtaggaggg
    41281 cgatgatcta agtaagaggc aatcaccggt gccggtaagc ccatcgtttt tgccagtaaa
    41341 gcgatacttt gctcgcgctg gctgcgggtt aacgcatcgg cctcactaaa ggttgccagt
    41401 acaccctgaa taaaagcgcc gtttttttct gcataggggc gagctgccag ataaaacgat
    41461 ccggtttgat tgagatcggt gccgtctttc aacacccgca cgccgccctg taataatgca
    41521 gcggagtagt agggatccca gatagcccag gcgtcaacgt taccttgctg gaacgcggcg
    41581 cgggcatcag ctggcgtcag ataagtgggc tggatgtcag taaacttaag tccggcctgg
    41641 cgtagtgcgc gcagtaaaag attgtgtgaa ctggaacctt tctggaacgc aactttgtgg
    41701 cctttaagat cggctgcggt tttgatcggg ctgttttctg ccaccagaat cacctcggct
    41761 tttggcttcg gcggctcgac gcccacgtac agcaaatcag ccccggcagc ctgggcgaag
    41821 attggcggaa tatccccggt actgccgaga tcaatactgc caacgtttaa cgcctcaagc
    41881 atttgcgggc cagcggggaa ctccacccac gagatttttg attgcggata gcgtttttcc
    41941 agtaactggt ggctttttgc caataccata ccaatactgc ctttctgata gcctatacgt
    42001 aacgcttcag gcgaggattc tgcagcaacc acaatcgtag agacgctaag caatcccacc
    42061 agcgccagtt taatgatgtt acgcatgggc attacctcgc agagaatgaa gatcaggaac
    42121 ctgaacatcg cggcggtgca atgcctgcca gaaagtttct agcgcggtat caagacgggt
    42181 ttgtaaattc ggcgtgaact ggggtttgtg atggtaatca attacttgtg agtcatcggc
    42241 aaacacgccg tgcaggatct cctgggcttt cagcgcactt aaaactggct taagggcata
    42301 atcgaccgcc agcagatggg ccaccgtacc gcccgtcgcc agcggtagca ccactttgcc
    42361 ttgcaacgca cgttctggca gcaggtcgag cagggttttc agcgcaccgg aataagcggc
    42421 tttatacaca ggcgtggcga caatcagccc gtcagcctgt tgcaactgtt cggtgaaggt
    42481 atggagtgcc ggactatcga aacgagcata aagcagatct tccgcggcga agttttgcag
    42541 attccagtga taaacctcaa catccaggcc atttagtttt tcccgcgcat attccagcaa
    42601 ggagctggag cgagaaggaa agcgaggact acccgccagg gtgatgacac gcatactctc
    42661 tccttataac caattgttct tgttttgtta acattgataa caattcgctc agtctgtcgg
    42721 agagacaaga aaatgccaaa tataaatttt gtgtatcttt ttc
//
LOCUS       DABSYT020000030        41435 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.030, whole genome shotgun sequence.
ACCESSION   DABSYT020000030 DABSYT020000000
VERSION     DABSYT020000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 41435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.030"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(121..768)
                     /gene="ychE"
                     /locus_tag="ID725_RS10060"
                     /old_locus_tag="pgaptmp_002012"
     CDS             complement(121..768)
                     /gene="ychE"
                     /locus_tag="ID725_RS10060"
                     /old_locus_tag="pgaptmp_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGV7850621.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(799..852)
                     /gene="ychE"
                     /locus_tag="ID725_RS10065"
                     /old_locus_tag="pgaptmp_002013"
     CDS             complement(799..852)
                     /gene="ychE"
                     /locus_tag="ID725_RS10065"
                     /old_locus_tag="pgaptmp_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGV7850622.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1245..3920
                     /gene="adhE"
                     /locus_tag="ID725_RS10070"
                     /old_locus_tag="pgaptmp_002014"
                     /gene_synonym="adhC"
     CDS             1245..3920
                     /gene="adhE"
                     /locus_tag="ID725_RS10070"
                     /old_locus_tag="pgaptmp_002014"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGV7850623.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4221..4838)
                     /gene="tdk"
                     /locus_tag="ID725_RS10075"
                     /old_locus_tag="pgaptmp_002015"
     CDS             complement(4221..4838)
                     /gene="tdk"
                     /locus_tag="ID725_RS10075"
                     /old_locus_tag="pgaptmp_002015"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGV7850624.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5442..5855
                     /gene="hns"
                     /locus_tag="ID725_RS10080"
                     /old_locus_tag="pgaptmp_002016"
     CDS             5442..5855
                     /gene="hns"
                     /locus_tag="ID725_RS10080"
                     /old_locus_tag="pgaptmp_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGV7850625.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(5999..6907)
                     /gene="galU"
                     /locus_tag="ID725_RS10085"
                     /old_locus_tag="pgaptmp_002017"
     CDS             complement(5999..6907)
                     /gene="galU"
                     /locus_tag="ID725_RS10085"
                     /old_locus_tag="pgaptmp_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGV7850626.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7109..8122)
                     /gene="rssB"
                     /locus_tag="ID725_RS10090"
                     /old_locus_tag="pgaptmp_002018"
     CDS             complement(7109..8122)
                     /gene="rssB"
                     /locus_tag="ID725_RS10090"
                     /old_locus_tag="pgaptmp_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGV7850627.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8214..9119)
                     /gene="rssA"
                     /locus_tag="ID725_RS10095"
                     /old_locus_tag="pgaptmp_002019"
     CDS             complement(8214..9119)
                     /gene="rssA"
                     /locus_tag="ID725_RS10095"
                     /old_locus_tag="pgaptmp_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGV7850628.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9232..9690
                     /gene="ychJ"
                     /locus_tag="ID725_RS10100"
                     /old_locus_tag="pgaptmp_002020"
     CDS             9232..9690
                     /gene="ychJ"
                     /locus_tag="ID725_RS10100"
                     /old_locus_tag="pgaptmp_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGV7850629.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9740..10582
                     /gene="purU"
                     /locus_tag="ID725_RS10105"
                     /old_locus_tag="pgaptmp_002021"
     CDS             9740..10582
                     /gene="purU"
                     /locus_tag="ID725_RS10105"
                     /old_locus_tag="pgaptmp_002021"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGV7850630.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10742..10826
                     /locus_tag="ID725_RS10110"
                     /old_locus_tag="pgaptmp_002022"
     tRNA            10742..10826
                     /locus_tag="ID725_RS10110"
                     /old_locus_tag="pgaptmp_002022"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10776..10778,aa:Tyr,seq:gta)
     gene            10871..11001
                     /locus_tag="ID725_RS10115"
                     /old_locus_tag="pgaptmp_002023"
     ncRNA           10871..11001
                     /ncRNA_class="other"
                     /locus_tag="ID725_RS10115"
                     /old_locus_tag="pgaptmp_002023"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11036..11120
                     /locus_tag="ID725_RS10120"
                     /old_locus_tag="pgaptmp_002024"
     tRNA            11036..11120
                     /locus_tag="ID725_RS10120"
                     /old_locus_tag="pgaptmp_002024"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11070..11072,aa:Tyr,seq:gta)
     gene            11167..11298
                     /locus_tag="ID725_RS10125"
                     /old_locus_tag="pgaptmp_002025"
     ncRNA           11167..11298
                     /ncRNA_class="other"
                     /locus_tag="ID725_RS10125"
                     /old_locus_tag="pgaptmp_002025"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11345..11476
                     /locus_tag="ID725_RS10130"
                     /old_locus_tag="pgaptmp_002026"
     ncRNA           11345..11476
                     /ncRNA_class="other"
                     /locus_tag="ID725_RS10130"
                     /old_locus_tag="pgaptmp_002026"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11660..12337)
                     /gene="narI"
                     /locus_tag="ID725_RS10135"
                     /old_locus_tag="pgaptmp_002027"
     CDS             complement(11660..12337)
                     /gene="narI"
                     /locus_tag="ID725_RS10135"
                     /old_locus_tag="pgaptmp_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGV7850631.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12337..13047)
                     /gene="narJ"
                     /locus_tag="ID725_RS10140"
                     /old_locus_tag="pgaptmp_002028"
     CDS             complement(12337..13047)
                     /gene="narJ"
                     /locus_tag="ID725_RS10140"
                     /old_locus_tag="pgaptmp_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGV7850632.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13044..14582)
                     /gene="narH"
                     /locus_tag="ID725_RS10145"
                     /old_locus_tag="pgaptmp_002029"
     CDS             complement(13044..14582)
                     /gene="narH"
                     /locus_tag="ID725_RS10145"
                     /old_locus_tag="pgaptmp_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGV7850633.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14579..18322)
                     /gene="narG"
                     /locus_tag="ID725_RS10150"
                     /old_locus_tag="pgaptmp_002030"
     CDS             complement(14579..18322)
                     /gene="narG"
                     /locus_tag="ID725_RS10150"
                     /old_locus_tag="pgaptmp_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGV7850634.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(18715..20106)
                     /gene="narK"
                     /locus_tag="ID725_RS10155"
                     /old_locus_tag="pgaptmp_002031"
     CDS             complement(18715..20106)
                     /gene="narK"
                     /locus_tag="ID725_RS10155"
                     /old_locus_tag="pgaptmp_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGV7850635.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            20445..22241
                     /gene="narX"
                     /locus_tag="ID725_RS10160"
                     /old_locus_tag="pgaptmp_002032"
     CDS             20445..22241
                     /gene="narX"
                     /locus_tag="ID725_RS10160"
                     /old_locus_tag="pgaptmp_002032"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGV7850636.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            22234..22884
                     /gene="narL"
                     /locus_tag="ID725_RS10165"
                     /old_locus_tag="pgaptmp_002033"
     CDS             22234..22884
                     /gene="narL"
                     /locus_tag="ID725_RS10165"
                     /old_locus_tag="pgaptmp_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGV7850637.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(22885..24279)
                     /gene="ychO"
                     /locus_tag="ID725_RS10170"
                     /old_locus_tag="pgaptmp_002034"
                     /gene_synonym="iatA"
     CDS             complement(22885..24279)
                     /gene="ychO"
                     /locus_tag="ID725_RS10170"
                     /old_locus_tag="pgaptmp_002034"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGV7850638.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            24465..24818
                     /gene="ychN"
                     /locus_tag="ID725_RS10175"
                     /old_locus_tag="pgaptmp_002035"
     CDS             24465..24818
                     /gene="ychN"
                     /locus_tag="ID725_RS10175"
                     /old_locus_tag="pgaptmp_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGV7850639.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLALRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(24862..25557)
                     /gene="chaC"
                     /locus_tag="ID725_RS10180"
                     /old_locus_tag="pgaptmp_002036"
     CDS             complement(24862..25557)
                     /gene="chaC"
                     /locus_tag="ID725_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGV7850640.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(25718..25948)
                     /gene="chaB"
                     /locus_tag="ID725_RS10185"
                     /old_locus_tag="pgaptmp_002037"
     CDS             complement(25718..25948)
                     /gene="chaB"
                     /locus_tag="ID725_RS10185"
                     /old_locus_tag="pgaptmp_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGV7850641.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26218..27318
                     /gene="chaA"
                     /locus_tag="ID725_RS10190"
                     /old_locus_tag="pgaptmp_002038"
     CDS             26218..27318
                     /gene="chaA"
                     /locus_tag="ID725_RS10190"
                     /old_locus_tag="pgaptmp_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGV7850642.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            27717..27824
                     /locus_tag="ID725_RS10195"
                     /old_locus_tag="pgaptmp_002039"
     CDS             27717..27824
                     /locus_tag="ID725_RS10195"
                     /old_locus_tag="pgaptmp_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGV7850643.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            28253..28360
                     /locus_tag="ID725_RS10200"
                     /old_locus_tag="pgaptmp_002040"
     CDS             28253..28360
                     /locus_tag="ID725_RS10200"
                     /old_locus_tag="pgaptmp_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGV7850644.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(28508..29362)
                     /gene="kdsA"
                     /locus_tag="ID725_RS10205"
                     /old_locus_tag="pgaptmp_002041"
     CDS             complement(28508..29362)
                     /gene="kdsA"
                     /locus_tag="ID725_RS10205"
                     /old_locus_tag="pgaptmp_002041"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGV7850645.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(29398..30207)
                     /gene="sirB1"
                     /locus_tag="ID725_RS10210"
                     /old_locus_tag="pgaptmp_002042"
     CDS             complement(29398..30207)
                     /gene="sirB1"
                     /locus_tag="ID725_RS10210"
                     /old_locus_tag="pgaptmp_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGV7850646.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(30211..30603)
                     /gene="sirB2"
                     /locus_tag="ID725_RS10215"
                     /old_locus_tag="pgaptmp_002043"
     CDS             complement(30211..30603)
                     /gene="sirB2"
                     /locus_tag="ID725_RS10215"
                     /old_locus_tag="pgaptmp_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGV7850647.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            complement(30600..31433)
                     /gene="prmC"
                     /locus_tag="ID725_RS10220"
                     /old_locus_tag="pgaptmp_002044"
     CDS             complement(30600..31433)
                     /gene="prmC"
                     /locus_tag="ID725_RS10220"
                     /old_locus_tag="pgaptmp_002044"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGV7850648.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(31433..32515)
                     /gene="prfA"
                     /locus_tag="ID725_RS10225"
                     /old_locus_tag="pgaptmp_002045"
     CDS             complement(31433..32515)
                     /gene="prfA"
                     /locus_tag="ID725_RS10225"
                     /old_locus_tag="pgaptmp_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGV7850649.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(32557..33813)
                     /gene="hemA"
                     /locus_tag="ID725_RS10230"
                     /old_locus_tag="pgaptmp_002046"
     CDS             complement(32557..33813)
                     /gene="hemA"
                     /locus_tag="ID725_RS10230"
                     /old_locus_tag="pgaptmp_002046"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGV7850650.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34027..34650
                     /gene="lolB"
                     /locus_tag="ID725_RS10235"
                     /old_locus_tag="pgaptmp_002047"
     CDS             34027..34650
                     /gene="lolB"
                     /locus_tag="ID725_RS10235"
                     /old_locus_tag="pgaptmp_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGV7850651.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            34650..35501
                     /gene="ispE"
                     /locus_tag="ID725_RS10240"
                     /old_locus_tag="pgaptmp_002048"
     CDS             34650..35501
                     /gene="ispE"
                     /locus_tag="ID725_RS10240"
                     /old_locus_tag="pgaptmp_002048"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGV7850652.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            35652..36599
                     /gene="prs"
                     /locus_tag="ID725_RS10245"
                     /old_locus_tag="pgaptmp_002049"
     CDS             35652..36599
                     /gene="prs"
                     /locus_tag="ID725_RS10245"
                     /old_locus_tag="pgaptmp_002049"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGV7850653.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            36724..38403
                     /gene="dauA"
                     /locus_tag="ID725_RS10250"
                     /old_locus_tag="pgaptmp_002050"
     CDS             36724..38403
                     /gene="dauA"
                     /locus_tag="ID725_RS10250"
                     /old_locus_tag="pgaptmp_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGV7850654.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(38458..38736)
                     /gene="ychH"
                     /locus_tag="ID725_RS10255"
                     /old_locus_tag="pgaptmp_002051"
     CDS             complement(38458..38736)
                     /gene="ychH"
                     /locus_tag="ID725_RS10255"
                     /old_locus_tag="pgaptmp_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGV7850655.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            39014..39598
                     /gene="pth"
                     /locus_tag="ID725_RS10260"
                     /old_locus_tag="pgaptmp_002052"
     CDS             39014..39598
                     /gene="pth"
                     /locus_tag="ID725_RS10260"
                     /old_locus_tag="pgaptmp_002052"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGV7850656.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            39715..40806
                     /gene="ychF"
                     /locus_tag="ID725_RS10265"
                     /old_locus_tag="pgaptmp_002053"
     CDS             39715..40806
                     /gene="ychF"
                     /locus_tag="ID725_RS10265"
                     /old_locus_tag="pgaptmp_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGV7850657.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT        10354 a        10754 c        10125 g        10202 t
ORIGIN      
        1 actgtaattt gagaagaata tttttaccta ttccttttgc ttttgataac tgcgcgggaa
       61 caaagtaaaa ataatgacga agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa
      121 ttaattaagc aggccgggga aaatcccctt aataccagta acgataaatt caatccccaa
      181 tgccatcagc aatagcccca taatacgcgt aatcacgttg atgccggtct ggcgtaaaac
      241 ccgtaccagc cacggtgcca tgcggaacaa tccccaacaa cataaagcga acaatgcaat
      301 agccacaaag aaaccaaaca gatagctaat gctgtgataa cgcgtacccc agacgatggt
      361 agaactgatc gcccctggcc ccgccatcaa cggcaacgcc aatggcacca caccaatact
      421 ttcacgtacc gcggtttctg atttttcttg cttgttctgt ttatcctcgc caagcttgcc
      481 gctgatcatc gacatcgcta tcgtcaccac caggatgccc ccggcgatac ggaacgaatc
      541 aattgatata ccaaaaagtt gcagaatcgt gtcgccgaga aaaagcgaga tccacaagat
      601 aatggccaca gacaggttgg ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata
      661 actggtcatg ctgataaaga cgggaataat ccctaccggg ttgaccagcg caaataaccc
      721 gatgaaaaat ttgaagtaaa cgggaaaatc aaaaagggtc tgaatcacgg ttagctccga
      781 agcaaaagcc ggataatgtt agccataaat aaggttgaaa agacgcgctg acaatacgcc
      841 ttttgacagc atttttcacc tcctaactac ttaaaattgc tatcattcgt tattgttatc
      901 tagttgtgca aaacatgcta atgtagccac caaatcatac tacaatttat taactgttat
      961 ctataatggc gaaaagcgat gctgaaaggt gtcagctttg caaaaatttg atttggatca
     1021 cgtaatcagt acccagaagt gagtaatctt gcttacgcca cttggaagtg acgcattaga
     1081 gataataact ctaatgttta aactctttta gtaaatcaca gtgagtgtgt gcgcgagtaa
     1141 gcttttgatt ttcatcggtt aagcaaatca tcaccgcact gactatactc tcgtattcga
     1201 gcagatgatt tactaaaaaa gtttaacatt atcaggagag cattatggct gttactaatg
     1261 tcgctgaact taacgcactc gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt
     1321 tcactcaaga gcaagtagac aaaatcttcc gcgccgccgc tctggctgct gcagatgctc
     1381 gaatcccact cgcgaaaatg gccgttgccg aatccggcat gggtatcgtc gaagataaag
     1441 tgatcaaaaa ccactttgct tctgaatata tctacaacgc ctataaagat gaaaaaacct
     1501 gtggtgttct gtctgaagac gacacttttg gtaccatcac tatcgctgaa ccaatcggta
     1561 ttatttgcgg tatcgttccg accactaacc cgacttcaac agctatcttc aaatcgctga
     1621 tcagtctgaa gacccgtaac gccattatct tctccccgca cccgcgtgca aaagatgcga
     1681 ccaacaaagc ggctgatatc gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc
     1741 tgatcggctg gatcgatcaa ccttctgttg aactgtctaa cgcactgatg caccacccag
     1801 acatcaacct gatcctcgcg actggtggtc cgggcatggt taaagccgca tacagctccg
     1861 gtaaaccagc tatcggcgta ggcgcgggca acactccggt tgttatcgat gaaactgctg
     1921 atatcaaacg tgcagttgca tctgtactga tgtccaaaac cttcgacaac ggcgtaatct
     1981 gtgcttctga acagtctgtt gttgtcgttg actctgttta tgacgcagta cgtgaacgtt
     2041 tcgcaaccca cggcggctat ctgttgcagg gtaaagagct gaaagctgtt caggacgtta
     2101 tcctgaaaaa cggtgcgctg aacgcggcta tcgttggtca gccagcctat aaaattgctg
     2161 aactggcagg cttctctgta ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg
     2221 ttgatgaaag cgaaccgttc gcacatgaaa aactgtcccc gactctggca atgtaccgcg
     2281 ctaaagattt cgaagacgcg gtagaaaaag cagagaaact ggttgctatg ggtggtatcg
     2341 gtcatacctc ttgcctgtac actgaccagg ataaccaacc ggctcgcgtt tcttacttcg
     2401 gtcagaaaat gaaaacggct cgtatcctga ttaacacccc ggcttctcag ggtggtatcg
     2461 gtgacctgta taacttcaaa ctcgcacctt ccctgactct gggttgtggt tcctggggtg
     2521 gtaactccat ctctgaaaac gttggtccga aacacctgat caacaagaaa accgttgcta
     2581 agcgagctga aaacatgttg tggcacaaac ttccgaaatc tatctacttc cgccgtggct
     2641 ccctgccaat cgcgctggat gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga
     2701 ctgaccgctt cctgttcaac aatggttatg ctgatcagat cacttccgta ctgaaagcag
     2761 caggcgttga aactgaagtc ttcttcgaag tagaagcgga cccgaccctg agcatcgttc
     2821 gtaaaggtgc agaactggca aactccttca aaccagacgt gattatcgcg ctgggtggtg
     2881 gttccccgat ggacgccgcg aagatcatgt gggttatgta cgaacatccg gaaactcact
     2941 tcgaagagct ggcgctgcgc tttatggata tccgtaaacg tatctacaag ttcccgaaaa
     3001 tgggcgtgaa agcgaaaatg attgctgtca ccaccacttc tggtacaggt tctgaagtca
     3061 ctccgtttgc ggttgtaact gacgacgcta ctggtcagaa atacccgctg gcggactatg
     3121 cgctgacccc ggatatggcg attgtcgacg ccaacctggt tatggacatg ccgaagtccc
     3181 tgtgtgcttt cggtggtctg gacgcagtaa ctcacgccat ggaagcttat gtttctgtac
     3241 tggcatctga gttctctgat ggtcaggctc tgcaggcact gaaactgctg aaagaatatc
     3301 tgccagcgtc ctaccacgaa gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg
     3361 cagcgactat cgcgggtatc gcgtttgcga acgccttcct gggtgtatgt cactcaatgg
     3421 cgcacaaact gggttcccag ttccatattc cgcacggtct ggcaaacgcc ctgctgattt
     3481 gtaacgttat tcgctacaat gcgaacgaca acccgaccaa gcagactgca ttcagccagt
     3541 atgaccgtcc gcaggctcgc cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg
     3601 caccgggcga ccgtactgct gctaagatcg agaaactgct ggcatggctg gaaacgctga
     3661 aggctgaact gggtattccg aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc
     3721 tggcgaacgt ggataaactg tctgaagatg cattcgatga ccagtgcact ggcgctaacc
     3781 cgcgttaccc gctgatctcc gagctgaaac agattctgct ggatacctac tacggtcgtg
     3841 attatgtaga aggtgaaact gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa
     3901 aagcgaaaaa atccgcttaa tcagtagcgc tgtctggcaa cataaacggc cccttctggg
     3961 gccgtttttt tgtttaccca aagcaacttt tccataaacc gacagcatta gccttcatca
     4021 tatttgcgac gatgtataac gcctaaacac agggatattg tactttacag gtcacaagtc
     4081 aacgtcggtg cttaagagcc ctgtgaggcg tatagcggcg ttaaaaactg ccgagaaggg
     4141 tgtatagccc ggaagaagtg cgtaaaacga actgacagga taaaagtgcc ctgctcaccc
     4201 tgtcagtaaa gaaattctta ttaatcgtgg cgatgccttt cctgaatagc cgttaatgag
     4261 ccgacttgta acgcctcttt atagtgttta cggcatacag aaacgtatcg ttcattacca
     4321 ccaataacca cctgctcacc ttcgttataa ggtctgcctg cttgatcaag acgcagcacc
     4381 atgcttgctt tacggccaca aaaacagatg gtttttaatt caaccagttt gtcggaccat
     4441 gccagtaagt attggctgcc aataaataat tcacctcgaa aatcggtacg taaaccataa
     4501 caaagtacgg gtatatcgag ttgatcgaca acctccgata attcatatac ttgttgtctg
     4561 gttaaaaact ggcattcatc aaccagtacg caatgaattg cctgctgctc atgttccgca
     4621 cgaatctcat caaataatga tgaattttgg ttaaataatt ttgcaggcga tgacaaacct
     4681 atacgcgaac tgactttccc ggcaccaaag cgatcatcaa tttctgccgt atatacgaca
     4741 gtgcgcatgc cgcgttcctg gtaattgtat gaagattgca acaatgctgt agacttaccc
     4801 gcattcattg cggaatagta gaaatatagc tgtgccatca gccacaggcc ctcaatgata
     4861 tgaaataaag ttggctggag tttatcataa ttcgtcagtt tttcagctca acagtatgca
     4921 cagaagtggt atatgcgttc tcccttacga agccttgcat aatccttctg agctatcatt
     4981 acaactgcct cgcttgttat aagcgggtaa atgactgctg gtaactattc acaatcttta
     5041 acctgttgcg caagtaatag ccctctgttg acctccagga gatagtgcaa tactaagtcc
     5101 ctgctcttat tgcgactgtt ctacttttca tcattcgctt aatagggaat tctcgtaaac
     5161 acgactaata cagaagactg aaaggtcgtc agcctacgat aatctcccca taaaatgtga
     5221 catgaatcag gaagttttaa cctcacgtgc tgcgaaatca tcggtacaaa tagggctata
     5281 tgccgcgtct tttctggcta attttatgaa aagatattta ttggcggcac aaaataaaga
     5341 acaattttga attccttaca ttcctggcta ttgcacaact gaatttaagg ctctattatt
     5401 acctcaacaa accaccccaa tataagtttg agattactac aatgagcgaa gcacttaaaa
     5461 ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg
     5521 aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg
     5581 ctgctgctga agttgaagag cgcactcgta aactgcagca atatcgcgaa atgctgatcg
     5641 ctgacggtat tgacccgaac gagctgctga atagccttgc cgccgttaaa tctggcacca
     5701 aagctaaacg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac ggcgaaacta
     5761 aaacctggac tggccagggc cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag
     5821 gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgc acttgcttaa
     5881 aatcccgcca tcggcgggat tttttattgt ccggtttaag acaatttaat aagaataagc
     5941 tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg ataacaccga tacggatgtt
     6001 acttcttaat gcccatctct tcttcaagcc aggctttaaa ttccgtgcca agggtgttat
     6061 gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg cagtcatggc
     6121 tcttcccttt catatgatag gcttcaaccg tttctttttc gattagcata tcaattgcgt
     6181 cggtgagctg aatttcatca ccagctcccg gaggggtttt tgccagcaac ggccaaatat
     6241 ccgcactaag tacgtaacga cccacaatag cgagattaga cggcgcaaca tccgcttttg
     6301 gtttttcaac cacgccaacc atcggtacgc tttcacccgg cgctaattca acgcctttgc
     6361 aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc tggctatgac
     6421 ccgtttcatc gaagcggcgg atcatctctg ccaggttatc ctgtgacaaa tcggattcat
     6481 attcatccag aataacgtca ggcaaaataa cagccacagg ttcatcaccc actaccgggt
     6541 gagcacacaa taccgcgtgt cccaggcctt tcgccagccc ctgacgaact tgcataatag
     6601 ttacgtgcgg cggacaaata gactgcactt catcaagcag ttgacgtttt acacgttttt
     6661 ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag tttttagatg
     6721 agtgtgtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc acgacgtatt
     6781 gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc gtcgccggca
     6841 acatcctggt tcctaatccc gcaacgggga taacggcttt tttgactttc gtattaatgg
     6901 cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt tctgtatcgc
     6961 atcccagtat atcagcaccc cggaggggct aagttctgaa aaggatgcga gttagcataa
     7021 ttaagacaat tacttatacg aatggcgacg atagtaccac cagccgacat tagctggtaa
     7081 tgcaaattta gcccgcgtta tcgtttgctc attctgcaga caacatcaag cgcagtcgac
     7141 caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat tgattcaaat
     7201 aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctgg tgttcgccgg
     7261 tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt tcgcgatgat
     7321 aatagccaac taatagcgga aactgccccg gcagattggc ctgacggagt aaatggttta
     7381 cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca agctgttcct
     7441 gtaataatcc gttaaacaat gcgcgtaata acaaggcagc aagtacgcca ttatgtccag
     7501 ctcgggttac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa
     7561 gcaccaggcc gggtttgtcc gcggcaacca attgacggta attaacccgg caatgggaaa
     7621 tcacctgctg aaccggcggt tgtagttcct gtaataattt cgccgctgcg gcagggttat
     7681 caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgc gaattaaaca
     7741 tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct ttaactggtt
     7801 tcagcaaaac atcttcaacg cccagacgta acgctttggc aatatctgcc atattttcag
     7861 tggcagatat caccagaact ggggtctggt cgcctctgtt acgtatatgc tccagcagtt
     7921 taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga gtgaaacctc
     7981 ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct cccaatgagg
     8041 aaaaccacga atccagaagc gagcgaaata cctgctcatc ttcaacaatg agaatctgtt
     8101 ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa tagtggcatg
     8161 ctattgccac ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc tggtcaaatg
     8221 ttggtgcgta ccaacggcaa aagttcgtcc attttctttt ccactgccag ctgcccggcc
     8281 gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca aacaggttga
     8341 atcagaatat cgggcggatc acctgccatg cggttccttt taaggcggtt ctccagcacc
     8401 tggatagaag tggtcataat ctctgttgcc gttggtgctg tcaccgcccg acgtgtcgtt
     8461 atactgccta acctttcttt cagacgcgca tgccacggca gagaatcacc attctcgcta
     8521 ttttcttcac tgacattaaa ggagagcaaa tcttgttgca tcaaatgagc atcgtgctgc
     8581 aggtcgaccg ctatcacaat atcagcaccc aatgcacgcg tgagcgaaat aggaattggg
     8641 ttaacgactg ccccatcaac cagccagtag ccgttatgcg caacgggtgc cattagtcct
     8701 ggaatactgc atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa ccataattca
     8761 cgcccagtac ttaaattggt ggcaacagcc gcgaagcgac gggaacaatt ttcgatctct
     8821 gtttccggca ttatttcgcg gtattgattg aagacacgct cgccgcgcag taacccaccg
     8881 cgctgccagg agagatccat cagtcgtaaa acatcccaat aactgaaaga ggtcacccaa
     8941 tcttccagcg cagataatcg atcgcatgca taggccgcgc ccaccagcga accaattgaa
     9001 catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat aacgccaata
     9061 tgcgaccaac ctctcgccgc gccagatccc agcgccagcc ctatttttat ctttctcatt
     9121 attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct cggttaacat
     9181 agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt tgtgtctcag
     9241 ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta tgtgtctggt
     9301 gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc ttttgtgatg
     9361 caagacgcag attatttaat aaagacctgg catccatctt gtggagcagc ggcgttacgt
     9421 gccgaattga ttgccggatt tgcgcatact gaatggttgg gattgaccgt atttgaacat
     9481 tgttggcagg acgccgacaa tatcggattt gtcagttttg ttgctcgatt taccgaaggt
     9541 ggcaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg tcaatggtac
     9601 tatattgacg gtacacgtcc gcagtttggc cgcaacgatc cctgcccttg tggttcaggt
     9661 aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag caaacactct
     9721 caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc
     9781 ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg agttaaatat
     9841 cgtacagaac aatgaatttg ttgatcaccg taccgggcgc ttttttatgc gcacggaact
     9901 ggaagggatt tttaatgatt ccaccctgct ggcggatctc gatagcgcat tgccagaagg
     9961 ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtgattctgg tcactaaaga
    10021 agcgcattgc cttggcgatt tgttgatgaa agccaattat ggcggcctgg atgtcgaaat
    10081 cgcggcagtg attggtaacc acgatacttt acgttctctg gttgagcgtt ttgatatacc
    10141 gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa agatggcgga
    10201 tgccattgat gcttatcaac ctgactacgt ggtgctggcg aagtatatgc gggtattaac
    10261 accggaattt gtgtcacgct tcccgaataa gatcatcaat attcaccatt ccttcctgcc
    10321 agcgtttatc ggcgcacgtc cttatcacca ggcctatgaa cgtggcgtga agattattgg
    10381 cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca tgcaggacgt
    10441 tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc gtgacgtcga
    10501 gaaaaacgtc ttaagtcgcg cgctctacaa agtactggcg cagcgcgtct ttgtttacgg
    10561 taatcggacg attattcttt aatcgccagc aaaaataact ggttaccttt aatccgttac
    10621 ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag cggcgcgtca
    10681 tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag cattaccccg
    10741 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
    10801 aggttcgaat ccttccccca ccaccatgat tcaccacagc gatgtggtaa cttcggtgag
    10861 attgtgaaag tttgcactca tcggggaagg gtgagaacct tcgactaagg ttcgactcga
    10921 gcgaaagcga gagaacgttt tcacaggcaa cggcccgaag ggtgaggagc gaagcgacga
    10981 ataatccttt ccccaccgcc atctcaagca ttacctcaaa atttgaatta cccctggtgg
    11041 ggttcccgag cggccaaagg gagcagactg taaatctgcc gtcatcgact tcgaaggttc
    11101 gaatccttcc cccaccacca tcactttcaa aagtccctga actctcaagc gaatccgtaa
    11161 ataaatattc tgcccaagcg gagaaggatg agaagcttcg actaaggttc gactcgagcg
    11221 ccagcgagag agcgttgccg caggcaacga cctgaagggc gaagcgcgca gcgctgagta
    11281 atccttcccc caccaccatc actttcaaaa gtccctgaac tctcaaacga atccgtaatt
    11341 aaatattctg cccaagcggg gaaggatgag aagcttcgac caaggttcga cttgagcgcc
    11401 agcgagagag cgttgccgca ggcaacgacc cgaagggcga agcgcgcagc gctgagtaat
    11461 ccttccccca ccaccatcgc ttcctgaaat aatcaaaacc tgttactccg agctaatttt
    11521 gcccaatggc accacgttta ccggacctac aatatcctcc cttgtaggtc ggataagcga
    11581 agcgcatccg acaactcacc ccgcaccgtg cccataaaaa aaccccgccg aagcagggtc
    11641 tatgtgaact aaaattcgct tagtgacgag cgcgcaccag ctggtactta cgtgtcagat
    11701 actccaccgg tacgctccag atgtgtacca gacgcgagaa cgggaacagc aggaataacg
    11761 tcatccccag caccaggtgt agacggaaga taaacgccac accatcgagg tgttgagaag
    11821 cgccaccgtg gaaggtcact accgactgcg cccagccaac cagtttcatc atctcgctac
    11881 cgtccatatg ttgggcggag aacggaatgg tcaacaggcc cagcgcgcac tggataacga
    11941 gcagcgacag gatcagtata tccgctccgg tagtcgttgc acgcacgcgt gggctgaaca
    12001 gacgacgttt aagcaaaagc acgccgccaa tcagacacag cacgccgctg gcaccaccag
    12061 caaacatcgc cattttctgt ttcacttcaa tcggcagcca ggcttcatac atccagtgcg
    12121 gcgtcaacat accgaagaag tgaccgacaa aaatccccag aataccgata tggaacaggt
    12181 tcgacgccag gttcatccct ttgcgatcca gcatctggct ggacgccgcg cgccaggtgt
    12241 actgcccgta gtcataacgc agccagctac caatcaggaa gaccgccccg gctatgtacg
    12301 ggtagatatc aaagaagaac atattcagga attgcattag tgctgtcctc cggtggtgat
    12361 attcagatat tgcggcgcga cggcaccggc aaagcgacgc tgatgagcag tgattgctga
    12421 atcaccgcag cctttgtcag caaagaattt aacctgctct tcttcccaaa cagcatccag
    12481 cgcctgcggc gtatcatcgc gcgcttcgtc ggcaattttt tccgccactt tgtcgctgtc
    12541 gatagcggta ttggccaatt tcagcagcag atcaaacagc acggcataac ggctttcacg
    12601 ctgttgcaga cgcgcactca gcaatgccag aatcggcgcg atatctttca aaccttccac
    12661 ggcttcgctt tgcggcagct gcgccaggta ctccagatac agcggcagat gatccggcaa
    12721 ttcgcggctg tttaactgca agccgtgctg ctcgtactgc gccagcaggt ccaccatcgc
    12781 ctggccgcgg tcgcgggatt cgccgtgcac atgttcaaac agcaacagtg acgtggcgcg
    12841 gccacggtcg aacagttcgc tgtactgcgc ctgggcatcg agcggatcca tcgtcgttaa
    12901 atcgcgcagg aaaatgccca gcgcatgggc atcctctttt ggcagatttt tcgacgcggc
    12961 aatcgcctca aacatctctt gttgatgctg ccataaggca gcatccggat attcaaggag
    13021 acgcgataca atcacgagtt cgatcatgga tgcggctccg ttttgctggt cacatcgatg
    13081 gcatcgatac gacggctgtt gaacagattg aatttggtat ctgaaccgtg gcagccatca
    13141 ccaaaggtaa agccgcagcc atttttctcc gggaaggctt cccgtgccag ttcacgatga
    13201 ctactcggca ccacaaagcg atcttcgtag ttagcaatcg ccagataacg gtacatctcc
    13261 tgtgcctggg cttcggtcag accgacctct tccagcgcac gggtatcaac tttaccgtca
    13321 acggtttcag cacgtttgta atgacgcatc gccagcatac gtttcagcgc gcgcagtacc
    13381 ggtttggtat caccggcggt cagcagattc gccagatact gtaccggaat acgcaggctt
    13441 tcgacgtctg gcagaatgcc gttgctaccc agctcacccg cgtctgctgc agactgaatc
    13501 ggagagagag gcggcacgta ccagaccatc ggcagtgtgc gatattctgg atgcagcggc
    13561 agcgccagtt tccattccat tgccatttta taaaccggcg actgctgtgc agcttcaata
    13621 acgctcagcg gaataccgtc tttaatcgcc tgctcgatga ctttcggatc gttcggatcg
    13681 aggaacacct ccagctgacg ctggtagaga tctttctcgt tctcggtgct ggctgcacgt
    13741 tcaatcgcgt cggcatcgta cagcagcacg ccaagataac ggatacgacc gacacaggtt
    13801 tctgagcaca cggtcggctg acccgcttca atacgcggat agcagaagat gcacttctca
    13861 gacttaccgc tcttccagtt gaagtagatt tttttgtacg ggcatccggt gatgcacata
    13921 cgccagccac ggcatttatc ctggtcgatc aggacgatgc catcttcttc acgcttgtaa
    13981 atcgcaccgc tcgggcaggt cgccacacat gccgggttca ggcagtgttc gcacaggcgc
    14041 ggcaaataca tcatgaaggt gttttcgaac tggctataca tcgccttctg gatgttgtcg
    14101 aagttcttgt ctttcgccag tttgtcaaac tcaccgccca ggtcatcttc ccagttcggc
    14161 cctttttcga ttttcgccat ccgttccccg gtaatcagcg aacgcggacg ggcaatcggc
    14221 tgcgatttgc tgccttccgg cgcggtatgc aggttctgat agtcaaaatc gaacggctcg
    14281 taataatcgt cgatccccgg cagatgcggg ttagcgaaga ttttacccag cagcatggca
    14341 cggttaccca tgcgcggttg cagtttgccg ttgattttac ggatccagcc gcctttgtat
    14401 ttttcctggt tttcccagtc agtcgggaag ccctggcccg gcttggtttc cacgttgttg
    14461 aaccacgcgt attccacgcc ttcacggctg gtccagacgt ttttacaggt aactgaacag
    14521 gtgtggcagc cgatgcactt atcgagattc agcaccatgc cgacttgtga acgaattttc
    14581 attttacgct ctcctgtacc tgatcattgc cttcgccatc taaccagtca atgttcttca
    14641 tcttacgcac tacaacaaac tcatcgcggt tagaacctac ggtgccgtag tagttaaagc
    14701 cgtatgccag atgggcatag ccgccgatca tatgcgtcgg tttcggcgtg atacgggtga
    14761 ccgagttatg gataccacca cgctgttggg taatttccga accaggcagg ttaacgatac
    14821 gttcctgcgc gtggtacatc atggtcatcc ctgccggaac acgctggctg acaaccgcac
    14881 gggcagtcag agcaccgttg ctgttgaata cttcaatcca gtcgttatcg gcgataccca
    14941 gatctttggc atcggcttca ctcaaccaga ccaccggacc accgcgacct aaagtcagca
    15001 tcagcaggtt gtcgctgtag gtggagtgga taccccactt ctggtgcggc gtcaggaagt
    15061 tgagcgcttt ttccgggttg ccgttggatt tctggcctat cacttctttc accgaacggg
    15121 tgtcgatcgg cggacgataa accagcaggc tttcaccgaa atcacgcatc cactggtgat
    15181 cctgatacag ttgctgacga ccagagagcg tacgccatgg gatcagctcg tgaacgttgg
    15241 tgtaaccggc gttgtaagaa acgtgttcat cttccagacc agaccaggtc gggctggaga
    15301 taattttgcg cggctgtgcc tgaatatcgc ggaagcggat cttctcgtct tctttattca
    15361 gcgccagatg cgtatggtca cgaccggtaa attcgctcag ggcagcccag gctttcacgg
    15421 ctacctgacc gttggtttcc ggtgccagtg tcaggatcat ctctgccgca tcaattgcgg
    15481 tgttcagcat cggctggcct ttcgccggac cttccgcttt ggtgtagttg agcttacgca
    15541 gcagatccat ctcgctctgg gtgttccagg caatcccttt accgccgtta ccgattttct
    15601 ccatcagcgg gccgatagag gtaaagcgtt cgtaagttgc cggataatcg cgctctacga
    15661 ccataatgtg cggtgcagtt ttgcctggga tcaggtcgca ttcgcctttt ttccagtctt
    15721 tcacatccag cggctgcgcc agttcagcgg cagagtcgtg ctggataggc agcgtgacga
    15781 tgtcggtttc tttacccaga tggccgacgc acacttcgga gaatttcttc gcgatggctt
    15841 tgtagatttc ccagtcgctt ttcgcttccc aggccggatc gaccgccgca gacagcgggt
    15901 gaataaacgg atgcatatcc gaagtattca tgtcgtcttt ttcgtaccag gtcgccgtcg
    15961 gcaaaatgat gtcggaatag agacaggtgc tcgacagacg gaagtccagc gtaaccacca
    16021 gatccagctt gccttccaga ccattgtcct gccagtccac ttcttccggc ttcacgccgc
    16081 cctgctgccc cagatcttta ccctggatac cgtgctccgt ccccagcagg tacttgagca
    16141 taaactcatg acctttaccg gaagaaccga gcaggttaga acgccagata aacaggttac
    16201 gcgggtggtt tttgccgttt tccggctgtt ccgcagcgaa acggatagaa ccctctttca
    16261 gggatttcac cgtatagtcc accggattca tcccggcttt ttctgcttcg ccagcgatag
    16321 tcagcgggtt agtgcctaac tgcggcgcag acggcagcca gcccatgcgt tctgcacgga
    16381 cgttaaagtc gatcagatgt ccggtatagc gggatttatc cgccatcggc gacagcagtt
    16441 cttccgcagt cacggtttca taacgccact ggctggagtg gttatagaaa taagaggtgc
    16501 tgttcatgtg acgcgccgga cgctgccagt cgagggcaaa cgccagcggc tgccagccgg
    16561 tttgcggacg cagtttttcc tgacctacat agtgcgccca gccgcccccg ctctgaccga
    16621 cacagccgca gaaaatcagc atgttgatca gaccacgata gttcatatcg aggtgatacc
    16681 agtggttcag ccccgcaccg acgataatca tcgaacgacc gtgcgtttta tcagcgttat
    16741 cggcaaattc acgggcgatg cgaataatct ggctgcgaga aacgccggta atctgctcgg
    16801 cccaggccgg ggtataagct ttcacatcgt catagctggt tgcacagtta acgtcgttca
    16861 ggccacgttc cagaccgtag tttgccagcg tcagatcata aacggtggtc accagggcgg
    16921 tgctgccatc agccagttgc aggcgtttca ccggcagttt gtgcagcagc acgttttcca
    16981 gttccacttt gttgaagtgt tccgtgccgt cgccaccaaa gtacgggaag cctacctcag
    17041 cgatctcatc ctggctaccc agcaggctca gttgcagctc ggtttcttcg ccagttttgc
    17101 cgtcgcgctg ctcaagattc catttgccct tctcgcccca gcggaagcca atagaaccgt
    17161 tcggcgcaac catttcgcca ttggtattaa aggcgacagt tttccattcc ggattgtttt
    17221 cctggcccag cgcatcaacc agatcagcag cgcgcagcat acgacctgca gcgtagtaac
    17281 cgtcgcgttc ttccagcatc accagcatcg gcatgtcggt gtagcgacgc acatagtcgg
    17341 tgaaatactg gcttgggttg tcgagatgga attcacgcag cattacgtgg cccatcgcca
    17401 gcgccattgc cgcatcggtg ccctgtttcg gtgccagcca cagatcgcac agtttggcga
    17461 tttcagcgta gtctggtgtg acggcaacag ttttggtgcc tttgtaacgc acttcagtaa
    17521 agaagtgagc atctggagta cgcgtctgcg gcacgtttga accccaggcg atgatgtagc
    17581 tggagttgta ccagtcagca gattccggta cgtcagtttg ctcgccccag gtttgcggag
    17641 acgcaggagg caggtcgcag taccagtcgt agaagcttaa gcaagtaccg ccaatcagcg
    17701 agagatagcg tgcacccgat gcgtaagaaa ccatcgacat tgccggaatt ggcgagaaac
    17761 cagcaacacg gtccgggccg tagtttttga tggtgtaaac gttagatgcg gcgatcagtt
    17821 cgttcacctc ctgccaggaa gaacgaacaa aaccaccgcg tccacgcgcc tgcttaaagc
    17881 ttttcgcttt atcggcgtct tcaatgatag aagcccatgc ctcaaccgga tcgctatgca
    17941 gcgccttcgc ttcacgccac attttcatca ggcgtttgcg catcatcggg tatttcaggc
    18001 ggttggcact gtaaagatac caggagtagc tggcaccgcg cgggcagccg cgaggttcat
    18061 ggtttggcag atccggacgg gtacgcggat agtcagtctg ctgggtttcc caggtgacca
    18121 gaccgttttt gacgtagatt ttccagctgc aggagccggt gcagtttacc ccgtgggtag
    18181 agcggacgat tttgtcatgc tgccaacgct ggcgatatcc atcctcccag tcacggttgg
    18241 tattgagaag ctggccatgc ccatcggcaa aggtttcacc cttctgtttg aagtagcgaa
    18301 accggtccag gaatttactc atcggttttc tcctgtggga gcctgtcggc cctctgataa
    18361 atcgacattg ctaatgttta agggcaaggt aacgctctga aacagcgtgg gaattgataa
    18421 cgatcaagag tgatggggat gaaaaataaa gtaaattcct aagatacccc tttaggataa
    18481 ttaacagtta cagcataaca tgctgtaaat taagacgttt tatacctgta gttatttaaa
    18541 ctattaagat ttttatgcaa atgggtatta aggagtattc cccatgggta acatattaat
    18601 ggttcttaat gaaatccggc gaaataaacc atataacatt agtatgttgt aactaaatga
    18661 cgcataaaaa aagcgcgaca tcatgccgcg caaaggataa tcaaatatta agatttattt
    18721 tttagaatgc cgaccatata ccgcccaggt aatcactacg caggcgatat agaaaatcaa
    18781 aaatactttc attgcgccga ctggcgaacc cgttaatgcc agcgagctac cgaacgcttt
    18841 cgggataaag aagccaccaa tcgcgccaat cgcagagatg aaacccaacg ccgccgccgt
    18901 gtcggttgcc gcttcacgca tcgcacgttc gtcagaaccc ccttctgctt tcacgcgatc
    18961 cattgtcagt ttacggaaga tcacggaaat catctggaaa gtggaaccac tacccagccc
    19021 agctgtcagg aacagcgcca ggaagaccgc gaagaacgcc atgaagcttc cgccctgccc
    19081 gtcagtcggt aaggtcagga acagcaggcc gctgaaaatc gccatcagaa taaagttcac
    19141 cagcgtgaca cgagttccgc ccagacggtc agataatgca ccacctgcag aacgcgccag
    19201 cgcaccaata aacggcccga agaaagcgta ttgcagaatc tgaacatccg ggaactgcgt
    19261 ttttgacagc atcgcaaagc ccgcggagaa gccgatgaag gaaccgaagg ttgccagata
    19321 cagcaggctc ataatccaca gatgaccccg tttgagtacc ggcaactgct ccttgatgga
    19381 ggctttcgag gtagcaagat cgttcatgcc aaaccacgcc gcaatggtga agatggcaag
    19441 gaacggcacc catatccagg acgcattcgc cagatacagc tcagtcccat ccggctgttt
    19501 gacgccctgg ctaccaaata ctgcgaaaat cgacagtgat accaccagcg gagcaaccaa
    19561 ctgcataacg ctaacgccca tgttgcccag accaccattc aggcccagcg cgccaccctg
    19621 cttctgtttc ggaaagaaga agctgatgtt tgccatactg gatgcgaagt tcgcgccagc
    19681 aaagccgcac agcagagaga tgatgatgaa gacgctataa ggcgtggagg tatcctgcac
    19741 ggcaaaaccc agccagacac aaggaataat cagaataccg gtgctgaacg ccgtccagcg
    19801 acgaccaccg aagataggaa ccataaagga gtatggaaca cgtaataacg cgccagaaac
    19861 cgaaggcaac gcagtcaaca taaatagctg atcggtcgta aaattaaaac cgactttcgg
    19921 taagttcacc gcaacagcgc tgaacaacat ccatacgcaa aacgccagca gcagacaggg
    19981 aacggaaatc cacaggttgc ggctggcaat acgttgaccg cgttgttgcc agaacgcagg
    20041 atcttccggt cgccaatctg taatgacagc tccagtagcc ctttcggggg cggatgagtg
    20101 actcatagac acctctgata ctcgtttcga tgtctgccac cttagtgtct gtagctaaag
    20161 gcaatttgat gtaaatcaaa cgataagata actttatcta tcattgatat ttatcattac
    20221 ccatagtgag tacagtgatt tcataaaaat tatgagattt ttcacggtgc tgtaaaatcc
    20281 ctacccttac cgatgtaaag ctactaacca cacggcaaat aaggagtaac tctttccggg
    20341 tatgggtata cttcagccaa tagccgagaa tactgccatt ccagaatgta tcgtcacatt
    20401 cattaaggtt attgctcatt taaagcctga aggaagaggt ttacatgctt aaacgttgtc
    20461 tctctccgct caccctggtt aatcaggttg cgcttattgt gttgctttct actgctattg
    20521 gactggcagg gatggcggtt tctggctggc tggtgcaagg cgttcagggc agcgcccatg
    20581 cgatcaacaa agcgggatcg ctgcgcatgc aaagttaccg tctgttggca gcagtgccat
    20641 taagcgagaa agacaagccc ttaattaaag agatggaaca aacggcattt agcgccgagt
    20701 tgactcgagc agcagaacga gacggacaac tggcgcaatt acagggttta caagattact
    20761 ggcgtaatga actcatccct gcactgatgc gtgcacaaaa ccgcgaaacg gtgtcagcgg
    20821 atgtcagcca gtttgttgcc gggcttgatc agctggtatc tggttttgac cgcaccacgg
    20881 aaatgcgcat tgagacagtg gtactggtcc atcgggtaat ggcggtattt atggcacttt
    20941 tactggtgtt cactattatc tggttgcggg cgcgactgct acaaccgtgg cggcaactgc
    21001 tggcaatggc gagtgccgtc agccatcgcg attttaccca acgcgcaaac atcagcgggc
    21061 gcaacgaaat ggcgatgctt ggaactgcgt tgaacaatat gtctgcagaa ctggccgaaa
    21121 gttatgccgt acttgagcag cgggttcagg agaaaaccgc cgggctggag cataaaaatc
    21181 agatcctctc ttttttatgg caggctaacc gccgtttgca ttcccgcgcc ccgctgtgtg
    21241 aacgcctgtc acctgtactc aacggcttac agaatttaac cctgctacgt gatatcgaac
    21301 tgcgggtgta tgacactgat gatgaagaga atcatcagga gtttacctgc cagccagata
    21361 tgacttgtga tgataaaggc tgccagctct gcccgcgcgg cgtattaccc gttggtgatc
    21421 gcggcacgac cctgaagtgg cggctggctg actctcatac gcagtacggt attttgctgg
    21481 cgaccctacc gcaggggcgt catcttagcc atgatcaaca acaactggtg gataccctgg
    21541 ttgaacaact caccgccacg ctggcgctgg atcggcatca ggaacgtcag caacagttga
    21601 tcgtgatgga agagcgtgcc accattgcgc gcgaactgca tgattctatt gcccaatctc
    21661 tctcttgcat gaagatgcag gtgagttgtt tacagatgca gggcgatgcg ctgccagaaa
    21721 gcagccgcga actgttaagt cagatccgta acgaactgaa tgcatcctgg gcgcagttgc
    21781 gtgaattgct caccacattc cgcttgcagc tcaccgagcc tggattacgt ccggcgctgg
    21841 aggcgagttg cgaagagtac agcgccaaat ttggcttccc ggtgaagctg gattatcaat
    21901 tgccgcctcg tctggtgcct tcgcatcagg caatccactt gttgcaaatt gcccgtgagg
    21961 cattaagtaa cgccctcaaa cattcgcaag cgagtgaggt cgtggtgacg gtggcgcaaa
    22021 acgataatca ggtcaaactg accgtccagg ataacggctg cggcgtgcct gaaaatgcca
    22081 tccgcagcaa tcactacggc atgataataa tgcgcgaccg tgcgcaaagt ttacgaggcg
    22141 attgccgcgt ccgccgtcgt gaatcaggtg gcaccgaagt ggtggtcacc tttattcccg
    22201 aaaaaacttt cacagacgtc caaggagata cccatgagta atcaggaacc ggctactatc
    22261 ctgctgattg acgatcaccc gatgctgcga actggcgtaa aacagcttat cagtatggca
    22321 ccagatatca ccgtggttgg cgaagcgagt aatggcgaac agggtattga actggcggaa
    22381 tctcttgatc ccgatctgat cctgttagat ctcaatatgc ccggcatgaa cggtctggaa
    22441 acgctggata aactgcgcga aaagtccctc tcagggcgca ttgtggtatt cagcgtctct
    22501 aaccatgaag aagatgtggt caccgcactg aaacgcggcg cggatggcta tctgttaaaa
    22561 gatatggaac cggaagatct gctgaaagca ttgcatcagg cagctgctgg cgaaatggta
    22621 ttaagcgaag cattaacgcc tgttctggcc gccagcttgc gcgctaaccg tgccacgact
    22681 gagcgcgatg ttaaccagtt aaccccacgc gagcgcgata ttctcaagct gattgcccag
    22741 ggtttgccga acaagatgat tgcccgccgc ctggacatca ccgaaagcac agtaaaagtg
    22801 cacgtcaagc acatgctgaa gaaaatgaag ctcaagtctc gcgtggaagc agcggtatgg
    22861 gtgcatcagg agcgcatttt ctgattacgg ttcccagcgc agttcgtcgt ttgacaatgc
    22921 gtcgaacggt tcgacaagcg ttagcgtgat ctcattggag gagacacgct gcccctggtt
    22981 atcttccacc accaccgaca atcgccagtg attgctcgcc ccttccccgt tctgccagtc
    23041 aggcatgatc agcgtccagc cctccgcgct gttggcttgt gcgcccggcg tcaaacttaa
    23101 tatctgcgta tcgccctgcc aaatcagttg ccgaataccg taacgactgc ggatttgtaa
    23161 tttcagcggc actgtttcgc caggttttag atcccacggc ggtgtcgcaa gaaacaccgt
    23221 taacgttttt cgctgtcggt actcaagagt cggtagatta tttcgctgcg gattatcata
    23281 gcgactacca cgtaacgact gactttcggc aacctcgccc gcagaaagtt gttttttgag
    23341 cggtacacca aagcggtaat taagattcag cccgaggtta ttttgattct cgccactttc
    23401 accctgttta tgctgggccg tcacagtgac taatggcaca ggggtgtaat ttaatcccag
    23461 actcaacgcg acgggattgt gataacccgt accagagtta aacaaatcaa cacgatcacc
    23521 aaaatactgt tctacgctga cactggtatt gaggtgttga tagaacggca tgcgcatccg
    23581 ggctgtcagg tcgtacccgc gcgccatccg ttgttcctgc gtggctgtct gttcatgcca
    23641 tgcagcaaac ggctgataaa agtttgccga tagtcgcaaa tattcgcccc acgcttcggc
    23701 accaaagccc gctcgctgaa gattttcatc cagcaagttg tcataaaaag tgttataacc
    23761 caccagccag ttgccgcgcg cccagcgttg cccaacgccc acattgctca ccagcccatc
    23821 atcctgctga gtaagaccaa gctggctcca ggtgagataa cgatcattat cttgtaacgg
    23881 cacaaaccag cttccacgac tgccggtaaa atgaccttcg ttatccactt tgacgtcaac
    23941 actggcattt ccccacggtg atagccacga ctctacgtgc tgattaacct gttgactaag
    24001 cgcgtcgcgg acttttccca atgcgaaagc ttttgcctgc tcgccagtat ccagcccgtt
    24061 atcattcata ctggtttcgc cgaaatcttt cacaatttca gcaaagtgtt tttccccatc
    24121 atgattttca ggcgccatgc ctaaatccgg cagaccatca ttgttattat caaagggatt
    24181 tgccgctttt tgctcgaagg tagattgtgc gttagctgta ccgcctgccg ccagcaagag
    24241 taaataaaac ggaataatgc gaggaacgaa acggctcaaa acgtcggtaa cttccggtaa
    24301 aaaatgaaaa aacatagcct taaagaataa cgattactgg caacttttgt cgtattttct
    24361 cgcgctttca ggaaaatcct taaaaatgtg accgattggt cagacgctgc agaagcagta
    24421 agatagaaga tgagtgtcta attccctgaa tacaggagta aataatgcaa aaaatcgtga
    24481 tcgttgccaa tggcgcacct tacgggagcg aatccttgtt taacagcttg cggctggcca
    24541 ttgcgttacg agagcaggag agcaatctgg ctctgcgtct gttcctgatg tctgatgcgg
    24601 tcacagccgg gttgcgcggg caaaaaccag gggaaggcta caacatacag caaatgctgg
    24661 agatccttac cgctcagaat gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg
    24721 ggattagtac acttcctctg attgatgggg tggaaatcgg tactcttgtg gaactggcgc
    24781 aatggacgct gtcagccgat aaagtgctca cattttaatg tttgaggggg cgtctgacgc
    24841 cccacgatga gggaagttta ctcaggcgaa tcccggccgc aacacaccat ccggataatt
    24901 ctccgccagc agttttttta ccgataccag caaatcattc agcccatcgt cctgcattcc
    24961 cagtttgata agctcctgtt ccagtgaaaa caggtattgt gcgttggttc ccagcggacc
    25021 gctcgccgcc gcaatcaacg gcgcgatgac ctgagcgcga gtgtcagatt catattctgg
    25081 atgtcgcggg tccataataa acacaatggc gtttactgtg cgtccatcat caagatcaag
    25141 ctgacaccag gttggcagat aacagccggt aatcatctcg cgcttccaca acagggttag
    25201 ttcctgctcc agcgtctctt ctggcagtcg ataggcgacg cctgtggtac gtccgccctc
    25261 ttttagtgca agcatccgtc ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag
    25321 gcaaaacgcg cgatgccatc caaccagtgt accggtgcac gactcggtaa actccagtgc
    25381 cggattccac atcaacgatc cataaccgaa gatccacacc ggtctctcat caggtcgaca
    25441 agccagcgtc gccgccagcg aagccgcccg ttgttctgct gaccataaga gtgattcttc
    25501 aatggcacca aatgccgttt tacaatcggc attcatcaag aaatcacgcg ttatcacctt
    25561 cttacacctc cgccactgca tgcttccctg cgacaacttt ttggtgtctt ccggacattt
    25621 ttactgctca ttttgaaacc actaacggga caatatgcaa tccgcggacc acttgcaagg
    25681 caacaccgca cgaatagctt taactaagct accggtttta cgatttttta tgccatttat
    25741 catcatcccc tttggcatat tcatgcttca cagcagccca ggccacttta tgcgcggttt
    25801 cttcgcgact ggcgtcatca cgccgatctt ctttatcttt atattgatcc catgcgctgt
    25861 tgaacgcttc tttatagata tcctgggcat gagacggtag aacgtgcttt acgctttccg
    25921 gcaggtcgct tttcgtttta tacggcatcg ttgactcctt ttacaaagtt gaacattaag
    25981 tgtggtcaac aatatctact cttgcaaaaa cataagaatt ttcatgtcag actgttctta
    26041 agaaacgcta aaacaaacat tgcctaaacg taatacttat ttttatgccc caatgagtaa
    26101 aaaatcgaca acggggtaaa aattcgtaaa atgttaccga tatccagaca ctttctgtta
    26161 tttttcctac ctatttttta cattttcgat aaattcatct gcaaagggat aaccataatg
    26221 tcaaatgctc aagaggcggt aaaaacccgc cacaaggaga cttcgcttat tttcccggtt
    26281 ctggcgctgg tagtgctgtt cctgtgggga agcagccaga cactaccagt ggtcattgcc
    26341 atcaatcttc ttgcgcttat tggtatttta agtagcgcct ttagtgttgt ccgtcatgcg
    26401 gacgtattag cccatcgcct gggagaacct tacggttcgc ttattcttag cctttcagtg
    26461 gttattcttg aagttagttt gatttcagct ttaatggcaa ccggcgacgc cgcgccaacg
    26521 ctaatgcgtg atacgctcta ttcgatcatt atgattgtta ccggtgggct ggtcggcttt
    26581 tcattattgt tgggcggtcg taagtttgcc acccaatata tgaatctgtt tggtattaag
    26641 cagtatttaa ttgcgctgtt ccccctggcg ataatcgtac tggtatttcc aatggctctg
    26701 cctgcggcga atttttcaac cggtcaggcg ttactggtag cattaatttc tgcggcaatg
    26761 tatggtgtat ttttgctgat ccagaccaaa acgcatcaaa gtttgtttgt ctacgagcac
    26821 gaagatgaca gtgatgacga cgacccgcac cacggtaaac cgtctgccca tagcagcctg
    26881 tggcatgcta tctggttgat tatccacctg attgccgtta ttgcagtcac caaaatgaac
    26941 gccagcccgc tggagacttt gctcgacagc atgaatgccc ctgtcgcctt tactggcttc
    27001 ctggtggcac tgttgattct gtcgccggaa ggtttaggtg cattaaaagc agtgttgaac
    27061 aaccaggtcc agcgcgcgat gaatctgttc tttggttcag tgttagcaac aatttcgctt
    27121 accgtacctg tcgtcacgct aattgccttt atgacgggta acgaattgca gtttgcactt
    27181 ggtgcgccag aaatggtggt gatggtggcc tctttagtgc tgtgccatat ctccttctcc
    27241 accggacgta ctaacgtgct caatggcgca gcgcatctgg cactgtttgc cgcctatttg
    27301 atgacgatat ttgcctgaca gttatatctc ctccgatgac ggaaaccggg ggagataata
    27361 taaattcctg cttattgtat ttcttcatac tacctggaca ggtcgttaat cagaattgcg
    27421 agcctgtacc acaacctgca cgaaaccact gcaacagctg ttgaaaagcc ggaatgtact
    27481 ctttattgca aacatttcag ggatgaagtg aagatgaatt gagacgtagt atccacacca
    27541 agccggcgtt atccggtgag gcgcaatgtt gcgggggttt tatccctggt ggcattggtt
    27601 gctggaaaga gaaaaccccc gcacgttgag aggtaaaacc tgacaacagg gctaatcttg
    27661 aaaccagaca cttcaagaat agccgcaaaa tgttgtcatt acaacaaggc ggctatatga
    27721 cgctcgcgca gtttgccatg actttctggc acgacctggc ggcaccgatc ctggcgggaa
    27781 ttattaccgc agcgattgtc ggctggtggc gtaaccggaa gtaaggtgaa aacgggcgtc
    27841 aggctgccgt gatggcaata tgcgcccgaa accggccgca ggggtaacag cctgcggcca
    27901 tttttgtctt tatcacttcc acaagtaaat attggcaacc cgagctggaa gcatcatcca
    27961 gagcactcat gaaacgactg taacagctgt tgaaaaaccg gaatgcactc ttcattgcaa
    28021 acatttcagg gatgaagtga agatgaattg agtcgtagta tccacaccaa gccggcgtta
    28081 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    28141 aaaacccccg cacgttgaga ggtaaaacct gacaacagaa cgggggctaa tcttgaacca
    28201 tcaacaattc aagaatagcc gcaaaatgtt gtcattacaa caaggcggct atatgacgct
    28261 cgcgcagttt gccatgattt tctggcacga cctggcagca ccgatcctgg cgggaattat
    28321 taccgcagcg attgtcggct ggtggcgtaa ccggaagtaa ggcgaaaacg ggcgtcaggc
    28381 tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggt aacagcctgc ggcctttttt
    28441 gtatccggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    28501 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    28561 tcatctgcct gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    28621 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    28681 cggctcgtgc cagctcagcc acctgagcac gacgaccgcc ggaagctgcg ccaaaaggat
    28741 cgcggcattg caatgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    28801 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    28861 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    28921 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    28981 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    29041 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    29101 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    29161 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    29221 gtttctgggt cacggttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    29281 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    29341 tgctaaccac tttttgtttc ataggatcgc cttaaccttg agtaaaatgt cgattaatta
    29401 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    29461 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    29521 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt ccgggttgaa
    29581 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    29641 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    29701 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatacgt ccagcatatg
    29761 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    29821 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    29881 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    29941 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    30001 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    30061 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    30121 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    30181 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    30241 gagtttaatg atgatgtaca gcaccaccaa cgtcagcggg aaggcaataa tgcgcgcctg
    30301 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    30361 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    30421 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    30481 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    30541 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    30601 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    30661 acgtcatgat accccgcgag gataaatgcc tgtcgcaccg cttcgccctg ctgccagcca
    30721 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    30781 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    30841 tcgccttgtt gaagatgagg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    30901 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    30961 atcgccagat gctgggcatt acgttgtgcc agggcgacag catcgggcat acgatctaca
    31021 gcggtaattt cgcagtccgg gcgctcgcga gccagcgcaa gcgcaatcgc cccggtaccc
    31081 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    31141 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    31201 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    31261 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    31321 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    31381 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    31441 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    31501 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    31561 cggtaacgcg cccctgcggg aagttgtaag tacggttacg gtcgctgcga tcgccactcc
    31621 ccagcaggtt acgacgggta gacgcttcgg cctgttgccg ttttgccatt tcagcagcgt
    31681 ggatgcgagc accgagtaca gaaagtgctt tagctttgtt tttatgttgt gaacgttcgt
    31741 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    31801 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    31861 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    31921 aagtatgaat acgaccctgc gattccgtag caggaacacg ctgcacgcga tgaccgccag
    31981 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    32041 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    32101 cgtaacggct gtacatacgg aacagatcgc ctgcgaacag cgctgcttcg tcgccgccgg
    32161 ttccggctcg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggcagtaaca
    32221 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    32281 gcgccatctc acgcatttca ggatcatcga gcatcatctg tgcggtttcg atatcttcct
    32341 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    32401 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    32461 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    32521 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    32581 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    32641 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    32701 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cttgctctgc
    32761 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    32821 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    32881 caggttgtgc gaaatgatgc tttgcagatc atcaacgcta taaagataag cattcgccag
    32941 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    33001 gttgcgacgg ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    33061 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatat cactcagggc
    33121 aatcacttcc gcgccgactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    33181 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    33241 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    33301 cgtacaagcc gcaaaagcga cagacacagc gctggcaccg atatctgttt cagtgcgtac
    33361 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    33421 acctttttgc gaatcggcaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    33481 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    33541 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    33601 gcggattaac gcctcttgca agttgtcctg ctcttcgacg ctaagataaa gctcagtgcg
    33661 gttacacgtc gacagcacca ccccgccctg caccatcggc tgtgcgagca gactgtccag
    33721 cgcctgatca agtttgtccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    33781 tttatggtta ataccgagcg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    33841 taaggttagt ctcggcgcat cataccgtat gcgcgagatc aataaaaggg agcgccccct
    33901 tttggagtaa ttgcccgaac ccgttagatt tctacaatta agacaacttg aacatagacg
    33961 atgactaatg gtgacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    34021 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    34081 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    34141 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    34201 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    34261 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    34321 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    34381 gaagagatga ttggcaaatt gactggaatg ccaattccgc tcaacagctt gcgccagtgg
    34441 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    34501 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    34561 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    34621 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    34681 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    34741 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    34801 tccgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    34861 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    34921 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    34981 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    35041 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    35101 cgtttgccga aggcgttggt gaaatactaa ccccggtgga tccgccagag aagtggtatc
    35161 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    35221 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    35281 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    35341 aatacgcccc gtcgcgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    35401 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    35461 aaggcgctaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    35521 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    35581 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    35641 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gaactagcac
    35701 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    35761 atggcgaagt cagcgtacaa attaatgaaa atgtacgcgg tggtgatatt ttcatcatcc
    35821 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    35881 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    35941 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    36001 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    36061 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    36121 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    36181 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    36241 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    36301 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagctctga
    36361 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    36421 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    36481 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    36541 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    36601 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    36661 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    36721 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    36781 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    36841 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggcaccccag
    36901 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctgacggg tgggtcacgc
    36961 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    37021 tttgggctgg caggactgct ggttgcgacc ttgctgtcgg ggatcttttt gattctgatg
    37081 ggtctggcac gctttggtcg cctgattgag tatattccgg tttccgtcac cttaggtttc
    37141 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    37201 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    37261 accattaatg tgggtgatgc tgccattggc attgtgacgc taggtattct tgttttctgg
    37321 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    37381 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    37441 gtcctggccg atggttctca gggtaacggt attccgcaac tgctaccgca actggtgctg
    37501 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg cacactgctg
    37561 cctgcggcat tctcaatggc aatgctcggc gcaatcgaat ctctgctctg cgccgtggtg
    37621 ctggatggta tgaccgggac gaagcacaag gcgaatagcg aactggttgg acaggggctg
    37681 gggaatatca tcgctccgtt ctttggtggt attaccgcta ccgctgccat cgcgcgttct
    37741 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    37801 gttattcttg ccctgctggt actggcaccg ctgctctcct ggctgccgct ttccgctatg
    37861 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcataaagt ggtcgacttg
    37921 ctgcgtcatg caccgaaaga tgacatcatt gtcatgctgc tgtgcatgtc gctgaccgtg
    37981 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    38041 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    38101 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    38161 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    38221 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    38281 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgca ctatggctcg cgcaggcatt
    38341 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    38401 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    38461 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtggcgaa cccaccagta
    38521 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    38581 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    38641 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    38701 cacgttaccg agtaacgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    38761 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    38821 tgtgcgggcg tgatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    38881 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    38941 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    39001 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    39061 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    39121 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    39181 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    39241 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    39301 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    39361 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    39421 aatcggtcat ccgggcgata aaaacaaagt tgttggtttt gtgttaggca aaccgcctgt
    39481 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    39541 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    39601 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    39661 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    39721 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    39781 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    39841 gtcgtaccaa tgcctgatcc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    39901 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    39961 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    40021 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    40081 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    40141 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    40201 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    40261 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    40321 atgtacatcg ccaacgtcaa cgaagacggt tttgaaaaca acccatatct tgaccaggtg
    40381 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    40441 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    40501 gagccgggtc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    40561 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    40621 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    40681 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    40741 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    40801 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    40861 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    40921 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacatcagt
    40981 gttcagatga cggatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    41041 tcagccccct tcacagccga tcaatattga ccacgctcca atatgctggc gagaaaagga
    41101 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    41161 ctggcaagat gaaaatgata accagtagca caataacaac gatagaccag aaaacccatt
    41221 gaccaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    41281 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    41341 caaaaatatt caccaaatta tttaaccgct gtttggtgta atgtttttgt catattaata
    41401 gtaacgaatt ggaataagta acgacaactt aggga
//
LOCUS       DABSYT020000031        39567 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.031, whole genome shotgun sequence.
ACCESSION   DABSYT020000031 DABSYT020000000
VERSION     DABSYT020000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.031"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..89)
                     /locus_tag="ID725_RS10270"
                     /old_locus_tag="pgaptmp_002054"
     CDS             complement(<1..89)
                     /locus_tag="ID725_RS10270"
                     /old_locus_tag="pgaptmp_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850658.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKP"
     gene            complement(96..989)
                     /locus_tag="ID725_RS10275"
                     /old_locus_tag="pgaptmp_002055"
     CDS             complement(96..989)
                     /locus_tag="ID725_RS10275"
                     /old_locus_tag="pgaptmp_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850659.1"
                     /translation="MVPNINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVAPDILV
                     LPVNVYHADFKIKINNAVEKT"
     gene            complement(1400..2341)
                     /gene="fabY"
                     /locus_tag="ID725_RS10280"
                     /old_locus_tag="pgaptmp_002056"
                     /gene_synonym="yiiD"
     CDS             complement(1400..2341)
                     /gene="fabY"
                     /locus_tag="ID725_RS10280"
                     /old_locus_tag="pgaptmp_002056"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGV7850660.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(2386..2823)
                     /gene="dtd"
                     /locus_tag="ID725_RS10285"
                     /old_locus_tag="pgaptmp_002057"
     CDS             complement(2386..2823)
                     /gene="dtd"
                     /locus_tag="ID725_RS10285"
                     /old_locus_tag="pgaptmp_002057"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGV7850661.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2820..3692)
                     /gene="yihY"
                     /locus_tag="ID725_RS10290"
                     /old_locus_tag="pgaptmp_002058"
     CDS             complement(2820..3692)
                     /gene="yihY"
                     /locus_tag="ID725_RS10290"
                     /old_locus_tag="pgaptmp_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGV7850662.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(3686..4285)
                     /gene="yihX"
                     /locus_tag="ID725_RS10295"
                     /old_locus_tag="pgaptmp_002059"
     CDS             complement(3686..4285)
                     /gene="yihX"
                     /locus_tag="ID725_RS10295"
                     /old_locus_tag="pgaptmp_002059"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGV7850663.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(4384..5169)
                     /gene="csqR"
                     /locus_tag="ID725_RS10300"
                     /old_locus_tag="pgaptmp_002060"
     CDS             complement(4384..5169)
                     /gene="csqR"
                     /locus_tag="ID725_RS10300"
                     /old_locus_tag="pgaptmp_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGV7850664.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(5203..6099)
                     /gene="yihV"
                     /locus_tag="ID725_RS10305"
                     /old_locus_tag="pgaptmp_002061"
     CDS             complement(5203..6099)
                     /gene="yihV"
                     /locus_tag="ID725_RS10305"
                     /old_locus_tag="pgaptmp_002061"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HGV7850665.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            6267..7163
                     /gene="yihU"
                     /locus_tag="ID725_RS10310"
                     /old_locus_tag="pgaptmp_002062"
     CDS             6267..7163
                     /gene="yihU"
                     /locus_tag="ID725_RS10310"
                     /old_locus_tag="pgaptmp_002062"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGV7850666.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            7187..8065
                     /gene="yihT"
                     /locus_tag="ID725_RS10315"
                     /old_locus_tag="pgaptmp_002063"
     CDS             7187..8065
                     /gene="yihT"
                     /locus_tag="ID725_RS10315"
                     /old_locus_tag="pgaptmp_002063"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HGV7850667.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            8081..9322
                     /gene="yihS"
                     /locus_tag="ID725_RS10320"
                     /old_locus_tag="pgaptmp_002064"
     CDS             8081..9322
                     /gene="yihS"
                     /locus_tag="ID725_RS10320"
                     /old_locus_tag="pgaptmp_002064"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HGV7850668.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            9389..10291
                     /gene="yihR"
                     /locus_tag="ID725_RS10325"
                     /old_locus_tag="pgaptmp_002065"
     CDS             9389..10291
                     /gene="yihR"
                     /locus_tag="ID725_RS10325"
                     /old_locus_tag="pgaptmp_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HGV7850669.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            10352..11227
                     /locus_tag="ID725_RS10330"
                     /old_locus_tag="pgaptmp_002066"
     CDS             10352..11227
                     /locus_tag="ID725_RS10330"
                     /old_locus_tag="pgaptmp_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGV7850670.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            11335..13371
                     /gene="yihQ"
                     /locus_tag="ID725_RS10335"
                     /old_locus_tag="pgaptmp_002067"
     CDS             11335..13371
                     /gene="yihQ"
                     /locus_tag="ID725_RS10335"
                     /old_locus_tag="pgaptmp_002067"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HGV7850671.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            13417..14802
                     /gene="yihP"
                     /locus_tag="ID725_RS10340"
                     /old_locus_tag="pgaptmp_002068"
     CDS             13417..14802
                     /gene="yihP"
                     /locus_tag="ID725_RS10340"
                     /old_locus_tag="pgaptmp_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850672.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            14842..16245
                     /gene="yihO"
                     /locus_tag="ID725_RS10345"
                     /old_locus_tag="pgaptmp_002069"
     CDS             14842..16245
                     /gene="yihO"
                     /locus_tag="ID725_RS10345"
                     /old_locus_tag="pgaptmp_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850673.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            16312..17004
                     /gene="ompL"
                     /locus_tag="ID725_RS10350"
                     /old_locus_tag="pgaptmp_002070"
     CDS             16312..17004
                     /gene="ompL"
                     /locus_tag="ID725_RS10350"
                     /old_locus_tag="pgaptmp_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HGV7850674.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(17095..18360)
                     /gene="yihN"
                     /locus_tag="ID725_RS10355"
                     /old_locus_tag="pgaptmp_002071"
     CDS             complement(17095..18360)
                     /gene="yihN"
                     /locus_tag="ID725_RS10355"
                     /old_locus_tag="pgaptmp_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850675.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(18462..19442)
                     /gene="yihM"
                     /locus_tag="ID725_RS10360"
                     /old_locus_tag="pgaptmp_002072"
     CDS             complement(18462..19442)
                     /gene="yihM"
                     /locus_tag="ID725_RS10360"
                     /old_locus_tag="pgaptmp_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGV7850676.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(19450..20160)
                     /gene="yihL"
                     /locus_tag="ID725_RS10365"
                     /old_locus_tag="pgaptmp_002073"
     CDS             complement(19450..20160)
                     /gene="yihL"
                     /locus_tag="ID725_RS10365"
                     /old_locus_tag="pgaptmp_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGV7850677.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(20377..22200)
                     /gene="typA"
                     /locus_tag="ID725_RS10370"
                     /old_locus_tag="pgaptmp_002074"
     CDS             complement(20377..22200)
                     /gene="typA"
                     /locus_tag="ID725_RS10370"
                     /old_locus_tag="pgaptmp_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGV7850678.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            22573..23982
                     /gene="glnA"
                     /locus_tag="ID725_RS10375"
                     /old_locus_tag="pgaptmp_002075"
     CDS             22573..23982
                     /gene="glnA"
                     /locus_tag="ID725_RS10375"
                     /old_locus_tag="pgaptmp_002075"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGV7850679.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            24156..25205
                     /gene="glnL"
                     /locus_tag="ID725_RS10380"
                     /old_locus_tag="pgaptmp_002076"
     CDS             24156..25205
                     /gene="glnL"
                     /locus_tag="ID725_RS10380"
                     /old_locus_tag="pgaptmp_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGV7850680.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            25217..26626
                     /gene="glnG"
                     /locus_tag="ID725_RS10385"
                     /old_locus_tag="pgaptmp_002077"
     CDS             25217..26626
                     /gene="glnG"
                     /locus_tag="ID725_RS10385"
                     /old_locus_tag="pgaptmp_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGV7850681.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            26738..26848
                     /gene="yshB"
                     /locus_tag="ID725_RS10390"
                     /old_locus_tag="pgaptmp_002078"
     CDS             26738..26848
                     /gene="yshB"
                     /locus_tag="ID725_RS10390"
                     /old_locus_tag="pgaptmp_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGV7850682.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(27038..28411)
                     /gene="hemN"
                     /locus_tag="ID725_RS10395"
                     /old_locus_tag="pgaptmp_002079"
     CDS             complement(27038..28411)
                     /gene="hemN"
                     /locus_tag="ID725_RS10395"
                     /old_locus_tag="pgaptmp_002079"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGV7850683.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(28600..29106)
                     /gene="yihI"
                     /locus_tag="ID725_RS10400"
                     /old_locus_tag="pgaptmp_002080"
     CDS             complement(28600..29106)
                     /gene="yihI"
                     /locus_tag="ID725_RS10400"
                     /old_locus_tag="pgaptmp_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGV7850684.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(29225..29391)
                     /locus_tag="ID725_RS10405"
                     /old_locus_tag="pgaptmp_002081"
                     /pseudo
     CDS             complement(29225..29391)
                     /locus_tag="ID725_RS10405"
                     /old_locus_tag="pgaptmp_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29688..30320
                     /gene="yihA"
                     /locus_tag="ID725_RS10410"
                     /old_locus_tag="pgaptmp_002082"
     CDS             29688..30320
                     /gene="yihA"
                     /locus_tag="ID725_RS10410"
                     /old_locus_tag="pgaptmp_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGV7850685.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(30702..33488)
                     /gene="polA"
                     /locus_tag="ID725_RS10415"
                     /old_locus_tag="pgaptmp_002083"
     CDS             complement(30702..33488)
                     /gene="polA"
                     /locus_tag="ID725_RS10415"
                     /old_locus_tag="pgaptmp_002083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGV7850686.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            33852..34784
                     /gene="yihG"
                     /locus_tag="ID725_RS10420"
                     /old_locus_tag="pgaptmp_002084"
     CDS             33852..34784
                     /gene="yihG"
                     /locus_tag="ID725_RS10420"
                     /old_locus_tag="pgaptmp_002084"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGV7850687.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(34825..36255)
                     /gene="yihF"
                     /locus_tag="ID725_RS10425"
                     /old_locus_tag="pgaptmp_002085"
     CDS             complement(34825..36255)
                     /gene="yihF"
                     /locus_tag="ID725_RS10425"
                     /old_locus_tag="pgaptmp_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGV7850688.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(36410..37036)
                     /gene="dsbA"
                     /locus_tag="ID725_RS10430"
                     /old_locus_tag="pgaptmp_002086"
     CDS             complement(36410..37036)
                     /gene="dsbA"
                     /locus_tag="ID725_RS10430"
                     /old_locus_tag="pgaptmp_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGV7850689.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(37053..38039)
                     /gene="srkA"
                     /locus_tag="ID725_RS10435"
                     /old_locus_tag="pgaptmp_002087"
     CDS             complement(37053..38039)
                     /gene="srkA"
                     /locus_tag="ID725_RS10435"
                     /old_locus_tag="pgaptmp_002087"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGV7850690.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(38116..38385)
                     /gene="yihD"
                     /locus_tag="ID725_RS10440"
                     /old_locus_tag="pgaptmp_002088"
     CDS             complement(38116..38385)
                     /gene="yihD"
                     /locus_tag="ID725_RS10440"
                     /old_locus_tag="pgaptmp_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGV7850691.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            38455..39039
                     /gene="mobA"
                     /locus_tag="ID725_RS10445"
                     /old_locus_tag="pgaptmp_002089"
     CDS             38455..39039
                     /gene="mobA"
                     /locus_tag="ID725_RS10445"
                     /old_locus_tag="pgaptmp_002089"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGV7850692.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            39021..39548
                     /gene="mobB"
                     /locus_tag="ID725_RS10450"
                     /old_locus_tag="pgaptmp_002090"
     CDS             39021..39548
                     /gene="mobB"
                     /locus_tag="ID725_RS10450"
                     /old_locus_tag="pgaptmp_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGV7850693.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT         9758 a         9978 c         9549 g        10282 t
ORIGIN      
        1 tttggtttat tattttttgg tacattacct attatcccgc taagaacctt ccttgctgca
       61 tggagatttt tttgcacagc acataacaca attgactaag tcttctctac tgcattattg
      121 attttaattt taaaatccgc atgatatacg ttgacgggca atacaagtat gtctggcgct
      181 acatctttgg ctgagtattt attggtgatc gacggccgtg aatccacgag aaaacttagc
      241 tgcaaattat gctctatatt accagctgtt tttgtggtgt gattaaacat agagatgcca
      301 cctctcattc tttcacacat atcctcaact ttttcacttc gttcttgtgg caatttggct
      361 ttaacgttaa tttgatgatt tttacccgtg cattccttaa ctacaagagg aatgttatct
      421 aaagtgccat acaggctaat aacatcacca ggatttttat catgtaagta agtttgatta
      481 atactcgaag cattcacaaa gaggtcaata ttataactgt ttataacttc acctacatca
      541 aatgtgtagt gcttactctg gaggctgcca tccttattta attgtatatc aatcaatata
      601 tccccggact ttgtcaccat atctttgcca taaaccattt gcatagccat ttcgaggaga
      661 tttttatcta actgattgcg ggcgttgagt aagcgactac caaataattt atatactgtt
      721 cccgtcattg ccagcgctgc gctttctggt actttgcgtt tccacagtga ggtttctcta
      781 tttttaaaca ggtcatattc attattactc gacaaaccat acttcagttc gttaacacaa
      841 tggaacagtt gttcctcacc ttccatttta aggcgatgtg aaaaatcatt taatagcgat
      901 tgcttcggtg attcattact cattagttgt ttttctaaat taacaacaga ataactttct
      961 tgtttaatga gattaatatt gggaaccatt acagcctcct tcccacggtt taagagcaaa
     1021 catgctacat aattatttat aacatccaga gcagtaaatg aatgatactg acaatgatgc
     1081 cagcccatta tctttgcata atgtttgtta gctatagatt tttaaagtat ttatgagaaa
     1141 actatggcac cagagggggt tttgactatc aaaaatcgct caaacctgaa ctaagatgat
     1201 gaattaagca ttatctgcga taattgctat acagctctac aaataatatt taaatgctcc
     1261 tgaacagtga tgtagcaaga cacgtatgga gccaacgaag aataaaaatt tatagagttc
     1321 ttattcaata taaccgaaat agccaaggac taatataaat gacctgaatg agagaggcag
     1381 caccgcccct ctccatcagc tactcttctt cgttcccgcc ctcttcatac gggccaaatg
     1441 gcttcgcggg cagaacgata tacgtgcctt caaacactgc acccggcgtc tcgtcgccaa
     1501 agatttcgac ctgcatctgc acccgtgctt ttcgtccgcg cgccagacgg tcgagatcgc
     1561 cgcttaaggc accaaggtcg gctaccgcat ggggtttacc gctaatcggt ttgctgtagc
     1621 ggatatgcgc atccgccaga ataatcgttc cgccgaggtg gcgttcgcgc agcatcagcc
     1681 agataagccc ccaaccggtg agcgtcgcca gtgagaataa actcccggca aacagcgtat
     1741 ggtgcggatt ctgattgccg gtttctggca tggtagtgat aaatttttgc ccggtatatt
     1801 gctgaatgcg cacgcccatt ttttcactaa gcgggatatg ttcgtaccac gcctgttgca
     1861 gctgcgcgca ccagtcgccg cgatgcaaaa tatcatccag agtggcgacg ggcttaatca
     1921 tcaaaaaatg gcgaatcggc gtggtggttg gcgtggtgat ttctccctga ttaacaaacc
     1981 ccagcttggc gaaaaactcc accgcgtctt cacgggcgct acaggtcacg cgcttaacgc
     2041 cttcctgacg cgccaccgac tccagggtca tcgccatcag cgtgcctaac cctttgtcct
     2101 gcacgtcggg atgaacggcc ataaagcgaa tggacgcttc attgtcggca ttaatataca
     2161 gtcggcctac cgccaccaga ttaccctgct cgtcgacgac catctggtga tgcgccatcg
     2221 catcccacgc gtcgcgttcc gaaccttttg gttgatgcag gggcttacgc aacatttccc
     2281 agcgaaactg atagtaacgc tctaattctt cttctgtttg tggaacccga aggtgataca
     2341 tagcgatact ctctcttgtt tcccgtgaca accctggaag ctggctcata cctgcaacca
     2401 gaatgtcacg gggccatcat tgaccagcga tacctgcata tccgcagcga agcgtcctgt
     2461 ttgcgtgttc atctcttgct gacggcagcg ttcgacgaaa tagtcatata acgcctctgc
     2521 gcgatccggt gatgcacctt tggagaaact tgggcgcatc ccccgttcgg tatctgcggc
     2581 gagggtaaac tgggaaacca ccagcacact gccgcccgcc tgttgcacgt tgagattcat
     2641 cttgccttcg gcatcgctaa agatgcggta gccgagcaca cgctcgcaca gacggtttgc
     2701 tttctgttcg tcgtcatcct tttcgacacc caataacacc aaaagtcccg caccaatttc
     2761 gcccgtcact tctccctcca cggtgacgct ggcacgggtt acgcgttgaa ttaatgcaat
     2821 catggttcgt cgtcttcttc ttgttcagct gcttgtttta gtttgcggta ttccccgaga
     2881 gtgacagtaa tttccgcgcc aagcaagacg atacaccatg tccagtagac ccaaacaaag
     2941 agaatgggga tcaccgccag cacaccgtaa atgagctgat atgacgggaa catggtgata
     3001 taaagcgcga aacctttctt tcctgcttcg aacaggagtg cagcaacaaa cgcgccgaca
     3061 atcgcgtcgc ggttaggtac gcggatggta ggaacaatgc tgtacagcaa ccagaaggag
     3121 atccacgaca acagcaacgg aaaaatacgc agcacgttgt cgatgacagt attgagatcg
     3181 ctcgcccagc gcagggagag caaataggaa ctgatcgcca gactggcccc tgccagcagc
     3241 ggccccagcg ttaaaatcat ccagtacacg gcaaacgagt aaattttggg tcgcgctcgt
     3301 ttactgcgcc agatggtatt caacgcgcta tcgatggagt acatcaacaa taacgcggtg
     3361 acgatcagcc cgcacgcccc aacggcggtc atcttgttgg aattggcaac aaattgttcg
     3421 atataccgct gaataacatc gccagtagca ggcagaaagt tggcaaaaat aaagtgacgt
     3481 aactgaatgc tgacgtcgga aaacatggga aaagcggcga aaagcgcaaa aacaacggca
     3541 accagcggca ctaatgagag caacgacaca taggcaaggt tacctgccag ggttgtcata
     3601 ttgtcctcat caatgcgttg ccagagtagt tttagccagg cccatagtgg acgggtacgg
     3661 tgcctggctt tgtcctgaat ggtttttagc ataacacctt cgcgaaatag tccgggatgg
     3721 tggttttatc tttcaccaga atactggtaa tgcccagctg attagctcct tctatattat
     3781 cggcgttatc gtcgaaaaag accgtatcgc tgggtgaaaa accttccgcc tgcaaaacat
     3841 gctggtaaat tcgagcttca ggtttgcgca tccccagatc ttgcgacaga tagatatggt
     3901 cagcagcatc acgaatttcc gggtattctt ccggccagaa ggtggtatgc aggcggttag
     3961 tattggaaag caccaccacg cgatgcccct gctcacgcag tttatgcatg atggcgatca
     4021 cttccgggcg cagcgcaaca aacaccgcct gccagccgtg agagaactgc tcgtagctta
     4081 gcggtagagc catctcatga cacagcgcct ctgcgaacgc ttcgtcgcta atttccccac
     4141 gctcatgctg atgaaacgcc tcccccatat gaaaactctt cttaagcgta gccagcggaa
     4201 tacgcgttaa atcgctccag gttcccagca cacggttaaa gtcgatatcg acaatcacat
     4261 tacctaaatc aaagatatag agcatttttg cctcctttcg cgccacaaga aattaactgt
     4321 agcgggaaag gatgggtttg gctatgtggc aaggtatgaa gccaggtcaa cagtcacaaa
     4381 aatctacgcg tcttcctggg gaaggatgat ttcaatttgg ctgtcttgta agcgtgattt
     4441 tagcgcagcg ggcggcagct cgtcggtaaa gagcgcggtg acctgtgcca cgttaccaat
     4501 ttcaaccgcc gccgaagcat gatacttagt gtgatcggca accagcagga tatttctcgc
     4561 gtgcgccatc atcgttttca ccacgttagc ttcgtttaca tcaaactcca tcaacgcgcc
     4621 atcgctctca atcgccccaa cgcttgttac cagataatca gcacgaaaat cggccacaaa
     4681 ggacgccgct gaagggccaa tgatcccgct attatgagag cgcaacgtac cgccgggtac
     4741 catcacttca aagcgcgggt tgtggtaaag aatatgcgcc acacgcaggc tgttggtgat
     4801 tatccgcaaa tgattatggt taagtaacgc ccgggcaaca tgctcaacgg tcgtaccaat
     4861 ggtgataaat attgttgaac catcaggaat atagtctgcc accgcttcgg caatcgcttt
     4921 tttttcctcg gtttgcgaaa cctcacgctg ctcgaacgcc gtattaacga cgctggaagc
     4981 ccgacccgcg ccaccgtgat ggcgcgtaat caggccttgc tcgcttaatt tacgaatatc
     5041 ccggcgcacc gtctgcgtgg agacatccag cagatttgcc agctcatcaa tattcatata
     5101 cccacgctcg gcgatcagca tgaggagttg gtcgtgccgc gggttaccgg ttagttcggt
     5161 aaggctcatg aaaattcctc gaaaaaccat caccctggca ttttatacaa aaagtgacaa
     5221 aaaagatcgg gtttgatcac agtcagggat cccggctcgt ccaccgggac gcgtgcattt
     5281 cgacgccgct acaccgctgg cgaagcggac tgactccgct aaatccccac ctgttgccag
     5341 caccaccgcc aatgcgccgt gaaaaacatc acccgcacct gtggtatcta ccacatcaac
     5401 tttgaaggcc ggttgatgtt gacgcccgcc attttccagc cagtcacagc ctgcgctgcc
     5461 ctgggtcaca tagacatgac catttgtgag cgtttgtgcc tgttttagcg cactggccat
     5521 ctctttcacg cccgttaagc gcgccagacc cggttctgaa aaggccgcat gatcgcttaa
     5581 tgccaccagc tcgctgatat cctgcggcgt aatatcaccg tccagaacgg tcatcacgcc
     5641 cgcctgccgg gccagagtga aggctttttt agcgccgtcg tgccagcgta catctgccag
     5701 cacaacatcc cactgagaga aatcaatttc ctccaaccac tctgcgtcag gcagcaggtc
     5761 cgggctgggg tagttaatga ttatccgctc gcctttggca tccaccatga tggcggattg
     5821 cgacgatttc gcctgtttat accgtttggt gtagcgggtg ttaacgcccc aggattccag
     5881 ctctgccagc aggctgttgc cggtgtcgtc atcaccgacg cgcccaataa aatcaacctg
     5941 cgctcccagt cttgccgccg caaccgctgc cgtcgccgct ggcccgccac caacttccgt
     6001 ataatttctc gccacgtatt taccgctctc cgtcggtaac ccttccacgt aatagatgcg
     6061 atccatcacg gttataccta cacaagcaac acgaatcatg gtcattcctt aaacattttg
     6121 ggatgcgttc attttaattt caccaattgt ttaaaaagtg acgtctgtca attttttgac
     6181 tataaattac aaatacgatc aaaaacagac aatcaaagcg tgtcataaat gacaaaaagt
     6241 gacatcactg tattcaggag aaggttatgg cagcaatcgc gtttatcggt ttaggacaaa
     6301 tgggttcgcc aatggcgagc aatttattgc agcaagggca ccaacttcgc gtctttgatg
     6361 tgaatgccga ggctgtgcgg catctggtag acaaaggcgc gactcccgcc gccaacccgg
     6421 cgcaggccgc taaagatgcc gaatttatca ttaccatgtt gccgaatggc gatctggtgc
     6481 gcagcgtgtt gttcggtgaa aacggcgttt gcgaaagctt atctaccgat gcgctggtca
     6541 ttgatatgtc gaccatccat ccgctgcaaa ccgataaatt gattgccgat atgcaagcca
     6601 aaggcttcaa catgatggat gttccggtag gccgtacttc tgcaaatgcc attaccggta
     6661 ctctgttact gctggctggc ggcaccgctg aacaagttga acgtgccacg ccgatcctga
     6721 tggcgatggg cagtgagttg atcaacgcag gcggtccggg catggggatc cgcgttaagc
     6781 tcatcaacaa ctatatgagc atcgcgctca atgcgctttc ggcagaagct gccgttttgt
     6841 gcgaagccct gaatcttccc ttcgatgttg ccgtcaaagt gatgagcggt accgccgccg
     6901 gtaaaggcca cttcaccact tcctggccga acaaagtcct cagcggggat ctttctcccg
     6961 ccttcatgat cgatcttgcc cataaggatc ttggcatcgc ccttgatgtc gccaaccagc
     7021 tgcatgtgcc aatgccgctg ggggccgcct cacgggaggt ttatagccag gcgcgcgcag
     7081 cgggtcgcgg tcgccaggac tggtccgcca ttctggaaca ggtccgtgtc agtgccggga
     7141 tgactgccaa agtaaaaatg taacgactgg ataaaggaat aaatgaatga ataagtacac
     7201 catcaacgac attacgcgcg catcgggcgg ttttgccatg ctggcggtcg atcagcgcga
     7261 agccatgcgc atgatgtttg ccgcggctgg ggcacccgct ccggtagccg atagcgtttt
     7321 aactgatttc aaagttaacg cggcgaagac cctctcgcct tatgcctcgg cgattctggt
     7381 agatcaacaa ttctgttacc gccaggtggt tgagcaaaac gcgattgcca aaagttgcgc
     7441 catgattgtc gccgccgatg agttcattcc tggcaacggt attccggtag atagcgtggt
     7501 cattgaccgc aaaatcaatc cgctacagat caaacaagac ggtggcaaag ccttaaaact
     7561 gctggtgctg tggcgtagtg atgaagatgc gcagcaacgc ctggatatgg tgaaagagtt
     7621 taacgaactg tgccactcac acggcctggt aagcattatt gagccagtcg tccgcccacc
     7681 acgtcgtggc gataaattcg atcgcgaaca agcgatcatc gatgccgcca aagagctggg
     7741 cgacagtggc gctgacctct acaaagttga aatgccgctt tacggtaaag gtccgcaaca
     7801 agagcttctc agtgcttcac aacgtctgaa tgaccatatc aatatgccgt gggtgatcct
     7861 ctcttccggt gtcgacgaaa aactgttccc gcgtgccgta cgcgtggcaa tgacagcggg
     7921 tgcatcgggc ttcctggctg gtcgtgctgt ctgggcatcg gtcgtcggtt taccagacaa
     7981 cgagctgatg ctgcgtgacg tttgcgcacc gaaattacaa caacttggcg atatcgtcga
     8041 cgaaatgatg gctaaacgcc gttaaaatgg ggatacaaaa atgaaatggt ttaacacttt
     8101 aagtcacaac cgttggctgg aacaggaaac cgatcgcatc tttgattttg gtaaaaattc
     8161 cgtagtaccg accggatttg gctggttagg caataaaggg caaatcaaag aagagatggg
     8221 cacccatctg tggatcacag ctcgtatgtt gcacgtttat tccgttgctg cggcgatggg
     8281 tcgacctggc gcttactcgt tggttgatca cggtattaaa gccatgaacg gcgcactgcg
     8341 cgataaaaaa tatggcggct ggtatgcctg tgtgaatgac gagggcgtgg tggatgcctc
     8401 caaacagggc tatcaacatt tctttgctct gctgggtgct gccagcgccg tcacaacggg
     8461 tcacccggaa gcgcgcaagc tgctcgatta caccattgaa attatcgaga aatacttctg
     8521 gagcgaagaa gagcagatgt gcctggagtc ctgggacgaa gccttcagca aaaccgaaga
     8581 gtaccgcggc ggcaatgcca atatgcacgc agtggaagct ttcttaattg tttatgacgt
     8641 aacgcatgac aaaaaatggc tggatcgcgc gattcgcgtg gcttccgtga ttatccacga
     8701 cgtcgcaaga aataatcatt atcgcgttaa cgaacacttc gatacccagt ggaatccgct
     8761 gccggattac aacaaagata acccggcgca ccgcttccgc gcgttcggag gtacaccagg
     8821 ccactggatc gaatggggcc gtttaatgct gcacatccac gcggccctgg aagcccgttg
     8881 cgaacaaccg ccagcatggc tgctagaaga tgccaaaggt ctgtttaacg ccaccgtgcg
     8941 tgatgcctgg gcacccgatg gtgcggacgg gattgtttat accgttgact gggaaggaaa
     9001 accggtggtc cgcgaacgtg tgcgttggcc tatcgtcgaa gcgatgggta cggcctacgc
     9061 gctctacacc gtcaccggcg atcgccagta cgaaacctgg tatcagacat ggtgggacta
     9121 ctgcatcaaa tacctgatgg actacgaaaa tggttcatgg tggcaggagc tggatgcgga
     9181 caataaggtc accaccaaag tctgggacgg caaacaggat atttatcacc tgctgcattg
     9241 cctggtgatc ccgcgtatcc cgttagcgcc aggcctggct ccggcagttg cggcgggtct
     9301 gctggatatt aacgcgaaat aactgactaa aaaaacgaca tatcaggctg cacgccgggt
     9361 atgtcgttat tcaataaagg tacctcgtat gcaaatcacc aacatgcatt gcagtggaca
     9421 gaccgtttca cttgccgcgg gggattatca cgcgaccatt gtgaccgtcg gtgcaggtct
     9481 ggctgaatta acctttcagg gctgtcattt agttattcca cataaacccg aagagatgcc
     9541 gctggcacat ctgggcaaag tccttattcc gtggccgaac cggatcgcca acggctgtta
     9601 tcgctatcag gggcaggaat atcagttacc cattaatgaa catggttcaa aagccgctat
     9661 tcatgggctg ctcgcctggc gtgactggca aatcagcgaa ttaaccgcca ccagcgttac
     9721 cctgacagct tttctgccgc ccagttacgg ctatccgttt atgctggctt cgcaggttgt
     9781 ttattcgctc aatgcacgca cgggtctttc cgttgagatc gcaagtcaga atattggtac
     9841 tgtgaccgca ccctatggtg tgggtattca tccttattta acctgcaact taacatctgt
     9901 tgatgaatat ctgttccagc ttccggcaaa ccaggtttat gccgtcgatg agcacgctaa
     9961 tccgacaacg cttcaccacg ttgatgagct ggatttaaat ttcacgcagg cgaaaaaaat
    10021 agccgcgaca aaaatcgacc acacctttaa aaccgctaat gatctgtggg aaataaccat
    10081 tacccatcca caacaggcgc tttcggttag cctttgttcc gatcaaccct gggtacaaat
    10141 ttatagcggt gaaaaattac agcgtcaggg actggcagtt gaacctatga gttgcccgcc
    10201 taacgccttt aattcaggtc tcgacttatt attactggaa ccagggaaaa cgcaccgcct
    10261 gttttttaat atttacgggc aacacaatta attttattaa gaaaagaacg agatcattgt
    10321 ttagagttgt atttacacaa caggaaatat tatggacttt tttacgttat tttcaccgga
    10381 tggggtcatg ctggaaacaa acgtggtatg tatcgttatc gcgttaatgt ttctttatac
    10441 ctttgtgggg atatgcgcgg gatttggcgg tggtttaacc actatgccgc tcgtcacgtt
    10501 aatgctcccc gtaaaaatgg caacaccact ctcggtaatt gtcggcaccg cgaccgcctt
    10561 gtacgccacc tggctgtcac gtaaagaaac ggattggcga tcggcaatgg tactgattat
    10621 tttctcgttt ctgggaatac ccgtcggttt atatgcactg tcgtatttac ccgaccattt
    10681 tatgaaagtg ggcttaggcg gattccttat cctgtactcc ttttacagta tgtttattcc
    10741 acgcctgccg atttatgacc gtagctggat cgccgcacct atcggtgcag tagcaggcgc
    10801 attaggcgca gctttctcaa ccaacggacc accgatggtt atctacggta tgctgcgtaa
    10861 cttaggcccg gcggcatttc gtggcactct gaacgctttc ttcaccgcaa acaacatcgc
    10921 ggtgatcggc gggctggcga ccagcggaat tttgactgtt tcgatcttta aactggtgct
    10981 gttttgtatt cccaccatga tcctcggttc actggttgga caatatgtgc ataaacgtat
    11041 ttcagtcaaa gtgttccgtg tgatggtctt tttattgctg attgcctccg gttccatgtt
    11101 gataaaaggc gcgctgggaa ttcccgccgc tgccgcactt ctacctccgt ttaccttgtt
    11161 agttatactg caactggtat ttggtaaaaa agtggcggca atccgtcagg ctgaagcaag
    11221 taaataatca cttatgcggg tttaaattta tatccgcaca cattctttaa ttaattaaac
    11281 ctgtaataac aggatggcat gaaatattag ctttatactc tatggagatt atttatggat
    11341 acgccacgcc cacaattacc agattttgaa tttcatcaga ataacgacag ttttacccta
    11401 cattttcgac aacgtcttat tttaacccat agcaaagata atccttgttt atggattggc
    11461 tcaggtatag cggacatcga tatgttccgc ggcaatttca gcattaaaga taaactacag
    11521 gagaaaattg cgcttaccga cgccatcgtc agccagtcac cggatggttg gttaatccat
    11581 ttcagtcgtg gttctgacat tagcgccacg ctgaatatct ctgccgacga tcagggccgt
    11641 ttattgctgg aactacaaaa cgacaacctt aaccacaacc gcatctggct gcgccttgcc
    11701 gctcaaccag aggaccatat ctacggctgc ggcgaacagt tttcctactt cgatctgcgc
    11761 ggcaaaccgt tcccgctatg gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat
    11821 gtcacctggc aggccgactg caaagaaaat gcgggcggcg actattactg gactttcttc
    11881 ccacagccta cgtttgtcag cacgcagaag tattattgcc atgttgataa cagttgctat
    11941 atgaacttcg actttagtgc cccggaatac catgaactgg cgctgtggga agacaaagca
    12001 acgctgcgtt ttgaatgtgc tgacacatac atctccctgc tggaaaaatt aaccgccctg
    12061 ctgggacgcc agccagaact gcccgactgg atttatgacg gagtaacgct cggcattcag
    12121 ggcgggacgg aagtgtgcca gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtc
    12181 aacggcatct gggcgcagga ctggtccggt attcgtatga cctcttttgg caaacgcgtg
    12241 atgtggaact ggaagtggaa cagcgaaaac tacccgcaac tggattcgcg tatcaagcag
    12301 tggaacaaag agggcgtgca gttccttgcc tatatcaacc cgtatgttgc cagtgataaa
    12361 gatctctgcg aagaggcggc aaagcgcggt tatctggcaa aagatgtcgc cggtggcgac
    12421 tatctggtgg agtttggcga gttctacggc ggcgttgtcg atctcaccaa tccggaagcc
    12481 tacgcctggt tcaaggaagt tatcaaaaag aacatgattg aactcggctg cggcggctgg
    12541 atggctgact tcggcgagta tctgcccacc gacacgtacc tgcataacgg catcagcgcg
    12601 gaaattatgc ataacgcctg gcccgcgctg tgggcgaaat gtaactacga agcccttgaa
    12661 gaaacgggca agctcggcga gatcctcttc tttatgcgcg ccggttctac cggtagccag
    12721 aaatactcca ccatgatgtg ggcgggtgac cagaacgtgg actggagtct ggacgatggc
    12781 ctggcgtcgg ttgtcccggc ggcgctatct ctggcaatga ccggacatgg cctgcatcac
    12841 agcgacattg gcggttatac caccctgttt gagatgaagc gcagcaaaga gctgctgctg
    12901 cgctggtgcg atttcagcgc cttcacgccg atgatgcgca cccacgaagg taaccgtcct
    12961 ggcgacaact ggcagtttga cggcgacgcg gaaaccatcg cccatttcgc ccgtatgacc
    13021 accgtcttca ccaccctgaa accttacctg aaagaggccg tcgcgctgaa tgcgaagtcc
    13081 ggcctgccgg ttatgcgccc gctgttcctg cattacgaag acgacgcgca gacctattcc
    13141 ctgaaatatc agtacctgtt aggtcgcgac attctggttg ctccggtaca cgaagaaggt
    13201 cgcagcgact ggacgctcta tctgccagag gataactggg tccacgcctg gaccggagaa
    13261 accttccacg gtggagaaat aactgttgaa gcgcccatcg gcaagccgcc ggtcttttat
    13321 cgcgccgaca gcgaatgggc tgcactgttc gcctcgttaa aaaacatcta atcatgcccg
    13381 cccgtgggga aactcacggg caaatggaga ataacgatga gtcacatcac aacggaagat
    13441 ccggcaactt tgcgcctgcc ctttaaagag aaactctctt acggtatcgg cgatctggcc
    13501 tctaacatcc tgctggatat tggtacgctt tatcttttga agttttatac cgacgttctg
    13561 gggctacctg gcacctatgg cggcattatc tttttgatct cgaaattctt taccgccttt
    13621 accgatatgg gaaccggcat catgttggat tcccggcgta agattggccc gaaaggtaag
    13681 ttccgccctt tcattttgta cgcgtcattc ccggtcacct tattggcaat cgctaacttt
    13741 atcggcacac cgttcgatgt cactggtaaa acggtgatgg ccactattct gtttatgctc
    13801 tacgggctgt ttttcagcat gatgaactgc tcctatggcg cgatggtgcc tgctattacc
    13861 aaaaacccca acgagcgcgc atcactggcg gcatggcgtc agggaggcgc tacgctgggc
    13921 ctgctgctgt gcacggtggg attcgtgccg gttatgaatc ttatcgaagg taatcagcaa
    13981 cttggctata tcttcgccgc cacgctgttt tcactgttcg gcctgctgtt tatgtggatc
    14041 tgctactcgg gcgtgaaaga gcgttatgtc gaaacccaac cagccaatcc ggcgcaaaag
    14101 cctggcctgc tgcaatcttt ccgcgcaatt gccggtaacc gcccactgtt cattctgtgc
    14161 attgccaacc tctgcacttt aggggcgttt aacgtcaagc tcgccatcca ggtctattac
    14221 acccagtacg tacttaacga tcccatcctg ttgtcatata tgggattttt cagcatgggc
    14281 tgtattttca tcggtgtgtt cctgatgcct ggcgcagtca ggcgttttgg taagaagaag
    14341 gtctatatcg gcggcctact gatttgggtg ctgggcgatc tgctcaacta tttcttcagc
    14401 ggcggttcgg tcagcttcgt ggcgttctcc tgcctggcat tcttcggctc agcgtttgtt
    14461 aacagcctga actgggcgct ggtttccgac accgtcgagt acggcgagtg gcgtaccggc
    14521 gttcgttcgg aaggaacggt ctacaccggc ttcaccttct ttcgcaaagt gtctcaggcg
    14581 ctggctggtt tcttccccgg ctggatgctg acgcaaatcg gttatgtgcc gaacgtggcg
    14641 caggctgacc acaccattga agggttgcgc cagctgatct tcatctaccc aagcgcactg
    14701 gcggtagtca ccattgtggc gatgggctgc ttctacagcc tgaacgagaa gatgtatgtc
    14761 cgcattgttg aagaaataga agcccgtaaa cgcacggcgt aataataaac aatgccctgc
    14821 ggggcgttat aaggagtgat tatgtctgac cataatccac tgacattaaa actgaatctg
    14881 cgggaaaaaa tcgcctatgg tatgggcgac gttggttcga atttaatgct tagcatcggt
    14941 actctgtatc tcctcaaatt ttataccgat gaattaggga tgccagctta ctatggcggg
    15001 attatctttc tggtggcgaa gttttttacc gcgtttaccg atatgctcac cggattttta
    15061 ctcgattcgc ggaaaaatat tgggcctaaa ggcaaatttc gtccctttat tttatacgca
    15121 gcggttccgg cggcgttaat tgcaacgctc cagtttatcg ccaccacttt tagtttgccg
    15181 gttaaaacga cgattgccac cgcgctgttt atgatgtttg gactttcata cagtctgatg
    15241 aactgctcgt atggtgcgat gatcccggcc attactaaaa acccgaatga gcgcgcgcag
    15301 ctcgcggctt accgccaggg tggtgcaacc atagggctat tgatttgtac cgtggcgttt
    15361 attccgttgc agtcgctttt ttctgactca accgtcggtt atgcctgtgc ggcactgatg
    15421 ttctccattg gtggctttat ttttatgatg ctgtgctaca gaggcgtcaa agagcattat
    15481 gtggacacag caccaaccgg acataaagcc agtattctca aatctttttg cgcgatattt
    15541 cggaatccgc cattgctggt tttatgcatt gctaacttgt gtaccctggc ggcatttaat
    15601 atcaaactgg cgattcaggt ctattacacc cagtatgtgc tgaatgatat taatttattg
    15661 tcgtggatgg gattcttcag tatgggatgc atcctcgtcg gcgtattact ggtgccatta
    15721 actgtaaaat gttttggtaa aaaacaggtt tatttagctg gcatggtgct atgggcggtt
    15781 ggtgatatcc tgaattattt ctggggaagt aactctttca ctttcgtcat gttctcttgt
    15841 atcgcctttt ttggcacggc ctttgtaaac agcctgaact gggcactggt gccagatacc
    15901 gtagattacg gtgaatggaa aaccggtatt cgtgccgaag gttctgttta taccggttat
    15961 accttctttc gtaaaatttc tgccgcactt gctggcttct tgccaggcat tatgctgacg
    16021 caaatcggtt atgttcccaa catcgcgcaa agtgatgcga cattacaggg tttgcgtcag
    16081 ctcattttta tctggccttg tgcactggca attattgctg cattgacgat gggattcttt
    16141 tacacactca atgaaaaacg gtttgcgttg attattgagg aaatcaacca acgcaaaaat
    16201 aaagagatgg cgacagaaga aaaaacggct tccgtaacgt tataaatgtc aatagccgct
    16261 atttccatcc tggtgggtgg cggcctccct acgtttaaaa atggacttat tatgaaaaat
    16321 attaatgcaa taattttatt atcttctctg acctctgcct cagtattcgc tggcgcatac
    16381 gtcgaaaaca gggaggcata taatctcgcc tccgatcagg gcgaggttat gctacgtgtg
    16441 ggttataact tcgatatggg cgcgggtatt atgttaacca atacctacac ctttcagcga
    16501 gaagatgaac taaaacatgg atataacgaa atcgaaggtt ggtatccgtt atttaaacca
    16561 accgataaat taaccatcca gcccggtggc ttaattaatg ataagagtat cggttcgggt
    16621 ggtgctgttt atctggatgt taactacaaa tttactccgt ggtttaacct gacagtgcgc
    16681 aaccgtttta accataataa ctacagctca accgatttaa acggtgatct ggataataat
    16741 gacagctacg agattggcaa ctactggaac tttattatta ccgataagtt ctcatatacc
    16801 tttgagccgc attattttta taacattaat gatttcaata gcggcaatgg cacgaagcat
    16861 cactgggaaa tcactaacac cttccgttac cgcattaacg agcactggct gccctatatt
    16921 gaattgcgtt ggttagatcg caacgtggaa ccgtaccacc gcgagcaaaa ccagattcgt
    16981 atcgggacga agtatttctt ctgatgtgaa actgccggat gcggcgtaaa cgccttatcc
    17041 ggcctacggt tgatacggtt ttcgttggcc ggattaagta catccggcca ttaattaagc
    17101 ttcagccgcc agcatctctt tcttacgttg gctaatacgc ttaaccagca ttacggaaac
    17161 caccgcacca cagaatgcga agcaggccat aatgccaaac acgattttgt agccaataat
    17221 ccccggattt aaatccagaa tgtagccata cagactgaag cagaacatcg ccggggcgta
    17281 accaataaag ctacccaacg ccatcgccgc gcctgtttta ttttcagcaa tttttgcttc
    17341 gccgataggt gcaaaaaata cggctcgctg tgtaaagact atcgcgccaa agcccagcgt
    17401 acatgccatc cctaaataga ccggcatact ttcgtgcggc agcataatca acagtacgag
    17461 cgcagcggta ctgatgataa aggtgtagca tagatattta ctcggcgatt tcaggatttt
    17521 atctgaaatc atgccaccaa tcggtccacc aatcattttc agacagtatt ggttaatgat
    17581 gccgtaagcc cccaccagcg caacgggcaa tagatagatg tttttcagga atggaatgaa
    17641 gaatgtcagg ccacagtaaa ccgcgtaaac gaagaagacg ttgaaagcga taagccagat
    17701 agttttgtct ttcagcaccg aggtcatact ggtgttttgc gatgccccgt cttctttttt
    17761 cacctcaacg gacggtgcct cttctttgtc attcaggaca aagaaaatag taatgcctac
    17821 ggcaatcaca ataagggaat agaaccagat gcctgcttta aaacctaata agccactgcc
    17881 aaaccaggta aatactgcca acgcagaaaa tgccaccacg gtatcgacaa tgccacgccc
    17941 tgtttcgaag aagccaaaca accgaccttg ttgttcgcta ttgcccaatc gacttaccga
    18001 cttgagcaag accggccagt tcatcatgtc gcaagtaacg ccaaatagcg cccagacaaa
    18061 gaggatgccc caataccccg gcattgtcgt caaataaaca cccagtaatc ctgtcgcaat
    18121 gagtgagaat gacatggtgt atctgcgcgg tagtttatcg gcaaaataaa tagacagaaa
    18181 aaagcccact gtggtaacaa atgagtttac cgacatagca ttaccaattt gaccattggt
    18241 caaatggaaa tattcctgca tcgggatata aaacgcatct ttcagcgacg gtaatttata
    18301 aattgtaccg ccacacagtg ttaatagact aaataacgcc catttctttt tcgtgagcat
    18361 aacatcccct aagcgatttt aaaaatcact gttttaaagg caataagata ccgaccattc
    18421 ttatggaatg gttttattaa ctgcgtatga gttaaaactt gttagtggtt cagaagatgg
    18481 ctcgcctctt gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca
    18541 tcttcttttt ctttacgtaa ccgctcatcc agcaaatgct tttctggcaa tggtgtttca
    18601 ctcatctggc gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgctgga
    18661 gcataataat caacctcttc ttccaggcca tatgccgtca cctgcggttc ttcatcgccg
    18721 aggcagataa ggtgtgttaa taactctttg aacggcatat ctccctgacc tgaaatacag
    18781 gctttatgac ccaggccacc ttgttcttta acgatcaagg catctttgat atggacctgg
    18841 gtaatatgcg gtgccatcgt ttttaaggcg tctatgggat gctcatttgc attgatcata
    18901 ttcgcaaaat caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc
    18961 aactcatgac tttttaaatc ttcatgctgt tcgatcgtaa aagtcaggcc gctatcctga
    19021 tacgcttccc gcacataggc aatgtcgtta gcgatgatcg ataatacgtc gcgcaaatta
    19081 ccttcataac gtggataaaa acgtacggac gatgccccag ttttcaacgc gatggcgacg
    19141 gcttcgtcaa tagatgcctt atctgaggcg ctggtttcaa tatgaatatc aaggttcaga
    19201 cggcgggctt tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa
    19261 cgctcgccat ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca
    19321 agtaaatcag caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt
    19381 aaataaagag gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc
    19441 ataaaacacc tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc
    19501 tatctggaca atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat
    19561 acccgcagaa ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat
    19621 caattttttt actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg
    19681 gttttcaagt tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata
    19741 gaaaacttta tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg
    19801 tgtcactgca atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt
    19861 aaacccccag gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag
    19921 ctccggactg taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa
    19981 gattttggat tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc
    20041 cagcagttct cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc
    20101 tttcaaccac cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat
    20161 tgtttaatcc ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag
    20221 caattaacgg cgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaaccac
    20281 agacttataa gggagatact cgcattggtg tgcgctcacg acaggcaaaa aaaaacctgc
    20341 catcgctggc aggtttttta tgactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt
    20401 ggcgcgacga cgatcgtttt ccgtcaggtg acgtttacga atacggatag aggtcggagt
    20461 cacttctacc agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat
    20521 aggcggaacc agaacaacgg cttcgtcagt accggaagca cgcatgttgg tcagtttctt
    20581 accggtcagg cagtttacag tcaggtcgtt agagcggcta tgaataccga taatctgacc
    20641 ttcgtaaact tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca aaccgaacag
    20701 cgcgaacgcg accgctttac cctgaccgtt agagatcagt acgccgttct gacgctgacc
    20761 cacttcaccc ggacgcacgt cgtcgtagtg gctgaaggtg gagtacagca gacccgtacc
    20821 ggaagtcatg gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta
    20881 gtcgagacgt acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc
    20941 gcccagcgcc tgcattacag aaccctgatg ctgttcttca acgtccagag tcacgttttc
    21001 atacggctct tgtttacgac cgtcgatttc acggaagata actttcggac gggataccgc
    21061 cagttcgaaa ccttcacgac gcatgttttc gatcagaaca gacaggtgca gttcgccacg
    21121 accagaaacg cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg
    21181 taccagttct ttgttcagac gatccaggat ctgacgagac gttacgaact taccttcttt
    21241 accgcagaac ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga
    21301 gagtgccggc agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc
    21361 gcccaggccc gtgatcgcca cgatatcgcc agcttccgcc agatcggttt cgatacgttc
    21421 cagaccgagg tggcccagca ctttaccaac tttcgcgttg cgggttttgc cttcgctatc
    21481 gatgatagta acctgctggt tcggcttcac tttaccgcgc ttgatgcggc caatgccgat
    21541 aacgccaaca tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag
    21601 gtcaacgtcc ggcgcaggaa cgtggtcaac aatcgcctgg tacagcgggg tcatgtcttc
    21661 cgccatatct tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg
    21721 gaagtccagc tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac
    21781 aacccaatca ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag
    21841 gccgtaagca aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc
    21901 gtcaaccacc agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa
    21961 gtcggcgtgc cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt
    22021 gtttttcgcg aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg
    22081 ctcttgggtt tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccag
    22141 ggtggtttta ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac
    22201 aactttgcct caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa
    22261 acaggatcac aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca
    22321 cggagcataa aaagggttat ccaaaggtca ttgcaccaat atggtgctta atgtttccat
    22381 tgaagcacta tattggtgca acattcacat catggtgcag cccttttgca cgatgttaag
    22441 catgataacg ccttttaggg gcaatttaaa agttggcaca gttttcgctt tatctttttt
    22501 acggcgacac ggccaaaata attgcagatt tcgttaccac gacgacaatg accaatccag
    22561 gagagttaaa gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag
    22621 tttgttgatt tgcgcttcac cgataccaaa ggtaaagaac agcacgtcac tatccctgct
    22681 catcaggtga atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc
    22741 ggctggaaag gcattaacga atccgacatg gtgctgatgc cagacgcatc caccgcagtg
    22801 attgacccgt tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc
    22861 accctgcaag gctatgaccg tgacccgcgc tctattgcga agcgcgccga agattacctg
    22921 cgttccactg gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc
    22981 gatgacatcc gtttcggatc atctatctcc ggttcccacg ttgctatcga cgatatcgaa
    23041 ggcgcatgga actcctccac ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg
    23101 aaaggcggtt acttcccggt tccgccggta gactctgctc aggatattcg ttctgaaatg
    23161 tgtctggtga tggaacagat gggtctggtg gttgaagccc atcaccacga agtagcgact
    23221 gctggtcaga acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt
    23281 cagatctaca aatatgttgt gcacaacgta gcgcaccgct tcggtaaaac cgcgaccttt
    23341 atgccaaaac cgatgttcgg tgataacggc tccggtatgc actgccacat gtcgctgtct
    23401 aaaaacggcg ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg
    23461 tactacattg gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc
    23521 accaactctt ataagcgtct ggtcccgggc tacgaagctc cggtaatgct ggcttactct
    23581 gcacgcaacc gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt
    23641 atcgaagtac gtttcccgga tccggcggct aacccgtacc tgtgctttgc tgccctgctg
    23701 atggccggtc ttgatggcat caagaacaag atccatccgg gcgaagccat ggacaaaaac
    23761 ctgtatgacc tgccgccgga agaagcgaaa gagatcccac aggttgcagg ctctctggaa
    23821 gaagcactga acgagctgga tctggaccgc gagttcctga aagccggtgg cgtgttcacg
    23881 gatgaagcga tcgatgcgta catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg
    23941 actccgcatc cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac
    24001 ccggcgcaac aacgccggga tttagttgcc gtggaaactt tcagcccatc tctgcatggg
    24061 cttttttctc cgtcaattct ctgatgcttc gcgcttttta tccgtaaaaa gctataatgc
    24121 actaaaatgg tgcaacctgt tcaggagact gctttatggc aacaggcacg cagcccgatg
    24181 ctgggcagat cctcaactcg ctgattaaca gtattttgtt aatcgatgac aacctggcga
    24241 tccattacgc caaccccgcc gcgcaacaac tgctcgccca aagctcccgc aaattgtttg
    24301 gtacgccgtt accggaactg ttgagctact tctcattaaa tattgagctg atgcaagaaa
    24361 gtctggaggc ggggcaaggt tttaccgata acgaagtgac gctggtcatc gacggtcgct
    24421 cgcatatcct ttctgtgacg gcccagcgta tgccggacgg catgatcctg ctggagatgg
    24481 cgccgatgga taaccagcgc cgcttaagcc aggaacagct acaacatgct caacaagttg
    24541 ctgcccgtga tttagtgcgt ggcctggcac atgagattaa aaatccgctt ggcggtttac
    24601 gtggcgcggc gcagctactc agcaaagcat tacctgaccc gtcactgctg gaatatacca
    24661 aagtgattat cgaacaggcg gaccggctgc gaaatctggt cgaccgtctg ttggggccgc
    24721 agctgcccgg tacgcgcgtt accgaaagta ttcacaaagt agctgaacgc gtggtaacgc
    24781 tggtgtcgat ggaactgccg gacaacgtgc ggttgattcg tgattacgat cccagcctgc
    24841 cggaactggc gcacgacccg gatcaaattg aacaggtctt gctgaatatt gtgcgcaatg
    24901 cgctacaggc gctggggccg gaaggcggtg aaatcattct gcgtacccgc accgcgtttc
    24961 aactgacctt acacggcgaa cgctatcggc tggcggcgcg gattgatgtg gaagataacg
    25021 ggccaggcat tccgcctcat ttgcaggata cgctgtttta cccgatggtc agcggccgcg
    25081 aaggtggcac cgggcttggc ttatccatcg ctcgtaattt gattgatcag cattcaggca
    25141 aaattgaatt taccagttgg ccagggcata ccgagttctc ggtttacctg cctatcagga
    25201 aataaaggtg acgtttatgc aacgagggat agtctgggta gtcgatgacg atagttccat
    25261 ccgttgggtg cttgaacgtg cgctcgctgg agcgggttta acctgtacaa catttgagaa
    25321 cggcgcggag gtactggagg cgctggcgag caaaacgccg gatgtgctgc tttcagatat
    25381 ccgtatgccg ggaatggacg ggctggcact gctcaagcag attaaacagc gccatccgat
    25441 gcttccggtc atcattatga ccgcacattc cgatctggat gctgccgtca gcgcctatca
    25501 acaaggggcg tttgattatc tgcccaaacc gtttgatatc gacgaagccg tggcgctggt
    25561 tgagcgcgcc atcagtcatt accaggaaca gcagcagccg cgtaatgttc agcttaacgg
    25621 cccaacgacc gatatcatcg gcgaagcgcc agccatgcag gacgtgttcc gtattatcgg
    25681 tcggctttcg cgttcttcta ttagcgtgct gattaacggc gaatccggca ccggtaaaga
    25741 actggtcgct catgccctgc atcgccacag tccgcgagcc aaagcgccat ttatcgctct
    25801 gaatatggcg gctatcccga aggatttgat cgaatcagaa ctgtttggtc acgagaaagg
    25861 cgcgtttacc ggcgcgaata ccattcgtca ggggcgtttt gaacaggctg atggcggtac
    25921 attatttctc gatgaaattg gcgatatgcc gctggatgtg cagacgcgtt tgctgcgcgt
    25981 gctggcagac ggtcagtttt accgcgttgg cggctatgcg ccggtgaaag tggatgtgcg
    26041 gattatcgct gccactcacc agaatctcga acagcgggtg caggaaggta agttccgtga
    26101 ggatctgttc caccgcctga acgttatccg cgttcatctg ccgccgctgc gtgagcgtcg
    26161 ggaagatatt ccccgcctgg cgcgccattt tttacaggtt gccgcgcgag aactgggcgt
    26221 agaagcgaag ttgctgcatc cggaaaccga agccgcgttg acgcgtctgg cgtggccagg
    26281 caacgtgcgc cagctggaaa acacctgccg ctggctaacg gtaatggctg ccggacagga
    26341 agtgttgatt caggatttgc ctggcgaact gtttgaatca acggttgcgg agagtacttc
    26401 gcaaatgcaa ccggacagtt gggcgacgct tcttgcgcag tgggcagaca gagcgctacg
    26461 ttccggtcat caaaatctgc tttccgaagc gcagccagag ctggagcgga cgttactgac
    26521 gaccgcgttg cgacatacgc aggggcataa acaggaggcg gcgcggctac tcggctgggg
    26581 ccgcaacacc ctgacgcgta agttaaaaga gctggggatg gagtgattca cggcttgtgt
    26641 gtaaagattg attattgagc gcaaattgct ggtattttgc gctttactgt tccgataagt
    26701 tcagtatgat cttgcccgga aaacggggag agtcattatg ctggaatcaa taattaatct
    26761 ggtatcgagt ggcgcagttg acagccacac accgcaaact gctgttgctg ccgtgctgtg
    26821 tgccgcgatg attgggctgt ttagttgagt ttcagattgc tgatgaagtg ctctttgttc
    26881 atgccagatg ctgcgtgaac gccttatccg gcctacaaaa gcaatatttt caacaggttt
    26941 tgtaggcctg ataagcgtag cgcatcaggc aatcttgttc tcagcacatt gatggcgctt
    27001 tcgttttcta ctttgtaaac gaagcgccat tcactattta aatcacccga gagaactgct
    27061 gcatccgcgc tttctggcgc agataggtat caaagcacat gcaaatgttg cggatcagca
    27121 agcgaccttt cgccgtcacc tgtattccct tctcatccac atccaccagc ccatcttttg
    27181 ctaacggggc gagcagcttg agatcttccg caaagtaatc agcgaagtgc aaatcccact
    27241 gtttctcaat aggggcgtaa tccagacgga agttgcagat gagcgactta atcacatcgc
    27301 ggcgaataca gtcatcacgc gttagcgcaa taccacgcca cagcgcattg ccttgttcat
    27361 ccacttgctg atagtactgc ttcaactctt tctggttctg cgcgtagcag tcgccaatca
    27421 tgctgatggc ggaaacgccc atccccagca gatcggtatc gccctgagtg gtgtagccct
    27481 ggaaattacg atgcagcacg ccttcacgct gggccaccgc cagctcgtca tccggacggg
    27541 caaagtgatc cataccgata aactgatagc ccgattgcgt caggaaggcg atggtttcct
    27601 gcaggatatc gagtttttgc tgcggactcg gcaggtcagc atctttgatt ttgcgctgag
    27661 cagcaaaaat ggtcggcaga tgcgcatagt taaagacgct cagacggtca gggtttagct
    27721 ccgccacgcg tttcagggta aaggcgaaac tttccggcgt ctgtttcggc aggccgtaaa
    27781 tcaggtcgat gttggtggag gtaaagccaa tctcacgcgc atggttaagc agcgcaaaga
    27841 taaactcttc atcctgctcg cggttaacca gacgttgcac ttctttgttg aagtcctgca
    27901 cgcccatact caggcgatta aagccctcgg cgcgtaaatg atcgagtaca tccagttcga
    27961 tttcccgcgg atcgacttcg atcgaaatct ccgcatcggc attgaactgg aagttttcgc
    28021 gcagcagctt catcaggcgg ctgatttgcg ctttattaag atacgtcggc gttccgccgc
    28081 cccagtgcag ctggctgacg tgacgcccgg cgaacagcgg tgcacgatgg acgatttctt
    28141 gctccagcgc gtccagatac tgatcggcct tgtgctgctg gcgagtaaca atcttattgc
    28201 agccgcagaa gtaacaaagc ttatggcaga acgggatatg tacgtagaga gataatggac
    28261 gctcaggata gcgcgccacg gcttgtaaaa acgcctgttc gccgaagtct tctgaaaact
    28321 ccagcgcggt cgggtacgag gtgtatcgtg gcccggaata gttatatttc tggatcaggg
    28381 ccagatccca gtcgatttgc tgtacagaca tgctcactcc ttccgatggc gtctctggcg
    28441 gctacggcgt accggcggat gcccgtttcg ggtcatcagc cggttgcgca gccacttttg
    28501 tcgccgcgac aaccgctgta gtttaacgaa taaccacacc agataacata taaccggaag
    28561 ggttatcagc agaacgggaa ggcccacggc ggcaatccgt tagttgcccc gcagcagacg
    28621 catcatgtct tcttgcttct cgtcttcttc ctcttcttcg tcatcgtcat aagagaggcc
    28681 gagtttctgc atcaactcat caatacgatc cagtttagca tccacccagg attgctcttc
    28741 ggcactcagg gtttcgcctg cttccagacg ttccagcagc gcatccagac gctcatccgt
    28801 ttctagtaat tccaactccg cctgcggtga aagcataggt ttctcactct tcggtttgtg
    28861 ctgtttggtg actttttcag tcacgcccaa tggaataggg gttttactgc caatacgtgg
    28921 atctttcggt gcgttctggc ctttgctgcc tgacgtgttg tttccgcccg ctgcgcggct
    28981 gcccggcgca tgaccacgac gttttttctg acgcttgcgg tcacgagctt cctgattcag
    29041 ctcttcgcgc gttttgcgac gcgcttttgc atgacctttg ctgcgtgatg gtgaagatgg
    29101 tttcataatg gttcgtcttt tagccgtttt attcagtata gatttgcggc ggaatctaac
    29161 agaaagcaag caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata
    29221 acccttaacg ggtcttacaa tccttgcagg ccgccaacac accctgtttt tccattagcc
    29281 ggaacaatcc gtgttgattc catttccgtt taattacgtc tccggacgtt tgtcttcctg
    29341 acaatcctct gtcttcgcct cctggcgctc ctgaccctca tcctgagtca ttgttcctgt
    29401 tagcgtcctc gctctatggg tgctacttta cgcctttgct ttaaacaaac aatcaaccgc
    29461 catcagtatt tgcacatttt cagagtttta tataaaagca attaactgat ttatatagct
    29521 tatttgacat gcatcgcaga attttctctg ctatttcgca taaagactat cggcaatgtc
    29581 tcacaaagcg tatggctttt acttacaacg ctcacgccga aaaatccgcc ttttattatg
    29641 gttcaggtat aatcgccgca aagctttgac tgacggagac gaccgctttg actaatttga
    29701 attatcaaca gacgcatttt gtgatgagtg cgcctgatat tcgccaccta ccttccgata
    29761 ccggaattga agtggctttt gcaggccgtt ccaacgcagg taaatccagc gcgctgaaca
    29821 cgctgactaa ccagaaaagc ctggctcgta cctcaaaaac ccctggacgc acccagctta
    29881 tcaacctgtt tgaagtggct gacggcaagc gtctggttga cttgcctggg tacggttatg
    29941 cggaagtccc ggaagagatg aagcgcaaat ggcagcgcgc gctcggcgag tacctcgaaa
    30001 aacgtcagag cctacaaggt ctggtagtgc taatggatat tcgccatccg ctgaaagatt
    30061 tggatcagca gatgattgag tgggcggtag acagcaatat cgccgtgctg gtactgctga
    30121 ccaaagcgga caaactggca agcggcgcac gtaaagcgca attgaatatg gtgcgtgaag
    30181 cggtgctggc gtttaacggt gatgtgcagg ttgaaacgtt ttcttcgctg aagaaacaag
    30241 gcgtggacaa gctgcggcag aaactggata cctggtttaa cgagatgcag cctgtagaag
    30301 aaacgcagga cggcgaataa ttttcttacc ttaatgctta tgccggatgt ggcgtatccg
    30361 gcccataaat tcaatagatt acaattctac cgttggcctg ataaacatgg tgcatcaggc
    30421 attacggatc tttcctttcg cccaataaaa aacgccccag tcattactga ctggggcggc
    30481 taaaatattc agccaaatcc gattacgtga agtaaaaggt ctgaaagata gaacatctta
    30541 cctctgtacc ctacgcgact aactttactc ttttttgttc agttcagaaa gcactttttg
    30601 taattttttt tcagttgttg cataggaatt tctaatagcc atcacaaaac gtgacagctt
    30661 atgttgctta cttacgaaaa aagggcatgt tcaggcgaat cttagtgcgc ctgatcccag
    30721 ttttcgccac tccccacttc caccagcaac ggcacatcca gacgggtaca gttttccatc
    30781 agttgatgaa tctgcttcgc aacggcatcg acatcatctc tatgaacttc aaataccagt
    30841 tcatcgtgta cctgcatgat catacgtaca cgcggttgct cagcctgtaa ccacgcatca
    30901 acggcaatca tcgcccgttt gataatgtcg gcggcggttc cctgcattgg cgcgttaatg
    30961 gctgcacgtt cagccgctgc acgacgagca ccattgctgg atttgatatc cggcagatac
    31021 agacggcgtc cgtccagcgt ttcaacgtag ccctgctctt tcgcctgagc acgggtgcgt
    31081 tccatatact ccagcacgcc agggtagcgt tcgaagtaaa ggtccatgta cttctgcgct
    31141 tctttacgtg gaatgttcaa ttgccgcgcc agaccgaaag cactcatgcc ataaatcaga
    31201 ccaaagttga tcgctttcgc gctacggcgt tgctcgctgg tgacggtttc cagtggcaaa
    31261 ccaaacactt ctgccgccgt tgcccggtgg atatcttttc cttccgcgaa tgcggtcagc
    31321 aagcctttgt cacgcgagag atgcgccata atgcgcagtt caatctgcga gtagtccgct
    31381 gagacaatca cataatcctc tggcgcaata aacgcctggc ggatacgacg accttcttcg
    31441 ttacgcaccg gaatgttttg caggttagga tcggttgacg ataaacgtcc cgttgcagtt
    31501 acagcctggt gataagaggt atgcacacgc ccggttttcg ggttgatcat cagcggcagc
    31561 ttgtcggtgt aagtcgattt cagcttcgcc agaccacgat actccagaat cacttttggc
    31621 aacggatagt ccagcgccag ttcttccagt acctcttccg acgttgacgg cgcgccaccc
    31681 ggcgttttct tcagcggttt aatgccctgt ttttcaaaca gaatggtttg taactgcttg
    31741 gtggaagaaa ggttaaattc ctcacctgca atttcatgcg ctttcttttc cagctcagcc
    31801 agacgaaggg tgagctcttc agaatgattg tgcagcactt tcggatcgat cttcacaccg
    31861 ttacgttcaa tgcgtgaaag tactggcacc agcggcattt cgatattctc gaagacgttc
    31921 aacggccctt tgtgtttttg cagatccggc cacattttca gatgcaactg caaggtgaca
    31981 tcagcatctt cggcggcgta acgtccggcc tcttcgaggg caatctggtt aaaagtcagt
    32041 tgatttttgc ctttacccgc aatctcttca aaagtgatgg ttttgtgctt caaccaacgt
    32101 tccgcgaggc tgtccatatc gtgacgcccg gcaacgctat tgagaatgta ggactccagc
    32161 atggtatcaa acgcaatccc acgcagctca atgccgtaat tcgccagaat accgcgatcg
    32221 tatttcaggt tttgcccgac cttcagcgcc ttttcatctt ccagcagcgg ttttagcaac
    32281 tcgagtgcac gctcgcgaga gatttgatcg ggcgcatcaa gataatcatg agcaaccgga
    32341 atatatgccg ctacgcctgg ctcgatagca aaagaaagcc cgaccaggtt agctgagatg
    32401 ttatcaaggc tgtcggtttc ggtatcaaat gcaaataccg gcgctttttc cagcttcgca
    32461 atccacgctt tcagtgtttc ttcatcaagg atggtgacgt agttgtcata agaaatcacc
    32521 gttgccgtca cttctggtgc ttcgtctgca acactggttt cctgtggctt cgcggctggt
    32581 tttgcccctt tggcctgtaa ccatttgccc gcttcgacat cagcagtcca gcgtttgaac
    32641 tcatactttt tgaacagccc caacaactct tccgctgccg gttgctgcac ttccagttgt
    32701 tcacaggtca gctccagttc aacgtcggtt ttaatcgtcg ccagctggta tgagagataa
    32761 gcaacttctt tgttttgctc gagcttcgct gccattgttt tcgcgccacg gaagctcaac
    32821 ccagcaattt tttctggctc ggcatacagc gtatccagcc cgccaagacc ttgcagcaat
    32881 gcctgcgcgg ttttttcacc gacgcccggt acgccaggaa tgttatcaga ggagtcaccc
    32941 atcagcgcca ggaaatcgat gatcagttct ggcggcacgc cgtacttatt caccacctct
    33001 tccggtccga ggatggtatt cgtcatggtg ttgataagcg taatatttgg cgtcaccagc
    33061 tgcgccatat ctttatcgcc agtgctgatc agcaccggac gcccggcttt ttcggcttcg
    33121 cgcgccagag taccgataac gtcgtccgct tctacgccag aaaccgccag cagcggcagt
    33181 cccatcgctt taaccatcgc gtgcaagggt tcgatttgtg ctcgcagatc gtccggcatt
    33241 ggcgggcgat gtgatttgta atgttcaaac agttcatcac gaaaggtttt tcccttggcg
    33301 tcaaagacca ccgctgcatg cgtcggttta tattgcatga tcagactgcg cagcatgttg
    33361 aggacaccat acatcgcacc ggtcggctcg cctgcgctgt tagtcagcgg gggaaacgcg
    33421 tgatatgcgc gataaagata agatgaacca tctacaagga taagtggatt ttgggggatc
    33481 tgaaccataa tgtccgtgcc tgtttatcag attatgggta aaggatgcca cagacaggat
    33541 aaaaacatga gtttttcacc gcttttcgtg aaaatctttt gtagatcttc tggatcgctc
    33601 gcaaatcgtc atgtggataa ctttgtgcat aaattattta catcgctttt aatttattat
    33661 catgcttttt acagcatgat gtttgtcttt gtttttcatg cagttaaaaa taaatccatc
    33721 cctaataatt gagataatgg gattttttct aatgtggata gatatgaatt atttttctcc
    33781 ttaaggatca tccgttattt gggtcgtttt ctgctaaaat ctgcgccttg cgcttcttta
    33841 gcgcactgtc tatggctaac cttttgaata aattcattat gacgagaata ctcgctgcga
    33901 taaccctttt gctgagtatc gtattgacca ttttggtcac tattttttgt tccgtcccga
    33961 tcatcattgc cgggattgta aaactattgc tacctgtgcc agtcatttgg cgaaaggttt
    34021 cgcgtttttg tgattttatg atgtattgct ggtgtgaagg tctggcggta ttactgcatc
    34081 tgaacccaca cctgcaatgg gaagttcacg ggctggaagg gttaagtaag aagaactggt
    34141 atctgcttat ttgtaaccac cgtagttggg cagatattgt cgtactgtgc gtgctgtttc
    34201 gtaagcacat tccgatgaat aaatatttcc tcaagcaaca gctggcctgg gtgccatttc
    34261 ttggcctggc gtgctgggcg ctggatatgc cgtttatgaa gcgttattcc cgcgcttatt
    34321 tattacgtca tccggagcga cgtggtaaag atgttgaaac cactcggcgt tcttgtgaaa
    34381 agtttcgcct gcatcccacc accattgtca atttcgttga aggctcccgt ttcacgcaag
    34441 aaaaacatca gcaaacccac tccactttcc aaaacttgtt gccaccaaag gcggcaggca
    34501 ttgcgatggc gctaaatgta ctgggtaaac aattcgataa actgcttaat gttacgttgt
    34561 gttatccgga caataaccgt cagccgttct tcgatatgtt aagcggtaaa ctgacgcgga
    34621 ttgtcgtaca tgtggattta cagcctattg ccgatgagtt acatggcgac tacatcaatg
    34681 acaaaagctt taagcgccat tttcagcaat ggctgaactc attgtggcag gaaaaagacc
    34741 gacttctgac gtcgttgatg tcatcacagc ggcaggacaa gtaaattttg ctcgcctctg
    34801 aatgagcaga ggcgagtgag tattttaatg aacaaaacgt ccggcacgag acataaattc
    34861 ttcttcgctc atctcctgtc cgttaaaaac aactttaccc ggtgtataac gcagctgcag
    34921 cgaggcggta ttgttgtcaa tcgtgattaa ctgaaacatc tgacctaatg tcatcatccc
    34981 gctgatttgt ttatcagcct gcttttgcgc tttttccgca tccattcctt cagataaatt
    35041 aagctgtttt gcggtttctg tgacgacatt aagcggtaac ttcacatcaa aattgagcga
    35101 tttgatatct ttgtttgtgg atgatgaaga tttggctggg tcggcaatac tgatatccag
    35161 attggcattt agttcgccga gtgcgttctt ccatcttacc ggttgtttaa tggtcggctc
    35221 actttgttgt aataacggta aatactcttt gaacaatgca gcattcactt cttgcagagc
    35281 aacttcatcg tttgctaatt cagggtgtgc agcaagctgc ttctgcatcg caatgttata
    35341 ctggatgata aattggcgca ccgctgaggg atcaatagat tcagcaatca atgaccactc
    35401 accactaccg agttgctgat tcccttttgt taacttatca agttcgtagg tcagattgac
    35461 attgatataa tctttgtctt taacgcgatc cagcctggtt tttgcaacga aagcatcgat
    35521 ttgggcaacg tcttccccgt gattgataat gttaatctga ttaaatttcg actcgctttc
    35581 accaaccgga aatttctttt cttctaatcg cgccagagaa tcaatggtaa aactttttgc
    35641 cgttactttg ttcattggtg ataattgcag aacaatatct tcgactttgc cttcaatatc
    35701 caggttagac aatgaactac tatcaccatt aaaagtaaac tggccctcac caaaagaaaa
    35761 ctttccatac tctgcagggg caacatctaa tgtcgtagag aatgagccac caaacgcgaa
    35821 tcgcaatgtc gcttccacgg gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag
    35881 ttcattattc actaagttca cttttgccgc tgccagtgct gggatgacat taccatgcat
    35941 taacattgtg atgggcaacg gaccgtgctc aacatccaca tcaaatacaa ctttctggcc
    36001 tggcttgata ttgagatcgg gtgcaccgtt atcgaatgtc atttgcattt gaaaacggga
    36061 attaaaaaag cctttgtcaa aattttgata acttaacgtc atatcgtagg catgttcacc
    36121 tttcttttta gcatcgttaa aatctttaat aaatttttcg acaccaggct gaatttgcgt
    36181 acctgtgtac catgtgccac caccccagat tacggctaac gcaacaataa cacctgtagc
    36241 tgacttacgt atcatataaa tattccctga taaaacagca aaactttgaa tatccactta
    36301 tgctgaaata atgtccagcg gcaggatgca ttatcagaat gtatttatat ttattaaata
    36361 ataaaaaaag cccgtgaata ttcacgggct ttatgtaatt tacattgaat tattttttct
    36421 cggacagata tttcactgta tcagcatact gctgaacaaa aacatccata ttgctggtat
    36481 ccataccctg cggattcagc tgatatttac cgttaacaaa catcgccgga acaccacgca
    36541 attgcacgtc agctgcagct ttttcctgct gagcgaccag agatttcacc acgaagctgt
    36601 tccacgccgc gtcgtactct tcacctttaa tacctgcgtt gataaataca tcgcggatat
    36661 cagatgctga acgaatggtc tgggttttct gtacgccttc aaacagcgga acagtcactt
    36721 tgtcttccac gcccagcgcc atcgccacag cccatgcctg agtcagatct ttgcccaggt
    36781 caccacccat gaagttgacg tggtatttag tcatcttcac gccttccggc agttttttct
    36841 tcacattatc agaaatatgc agaacttctt caaactgata gcagtgcggg cagaagaaag
    36901 agaaaaactc cagcacttgc ggcgcgccag ctaccggttt ttccagggta gtgtactgtt
    36961 taccatcttc atactgcgcc gccgatgcgc taaacgctaa aactaaacca gccagcgcca
    37021 gccaaatctt tttcatgatc tactctctcc gattaataca taggtgttaa ttgcaaaggg
    37081 ggttcttgta gaacttttgc ctgttctata aaagtcgccg tctgtcgtag ccagtaatct
    37141 tccccggtta accacgggaa atttttcggg aacgcgggat cagcccaacg ccgcattagc
    37201 caggcaagat aataaaccaa acgcatggcg cgtaaaggtt caatcagtcc gatttcagcg
    37261 gtgtcgaact cgctaaattc ttcataagct tcaataatag tttccagttg catccgctgc
    37321 tcggctttat cgccattgag caacatccac aaatcctgaa tagctggacc attacgtgca
    37381 tcatccagat caacaaacat tggaccatcg cgccagagaa tattcccggc gtggcagtct
    37441 ccatgtagcc gcagaacggt gaaatcttcc cgccagtgtg ctgtaacggc ggcgatcagc
    37501 tcatctgtcg ctttcaggaa tgccgctttc aacccggaag gtatcagtgt agcgtcctca
    37561 aacagcttgc gtggctcaat gagatattca ttcagaccga tggtcgggcg atggataaaa
    37621 agctgtttgc gccccgtctg gtgcatacgc cctaaataac gcccaaccgc ttccatctga
    37681 tcgatattat cagcttcgaa ctggcgacca ccgacgcttg gaaaaacagc gaaataaaat
    37741 ccctgatgat tcaataaagt ctgaccgtta aaagccacag gtgctgcaac cggaacttca
    37801 tcatttacca gctgcaacgc aaattgatgt tcttcgagga tttgatcggc tgtccaacgt
    37861 tcaggacgat aaaatttgac gacaaaacgt cgacgatctt cgtcctgaaa ttgatagaca
    37921 cggttttcat agctgttaag cggggtaaga ccggaatcca cccggatccc ttgctcaaac
    37981 agagcgtcca tgatggtatc cgggtgtagt gtctggaaag taaaagcgct gttgttcatc
    38041 ccatcatccg gaaaacatta cgaatgatga aggatatcat ttcacgatga ttttggtggc
    38101 gccgcttaca agcttttact ctttaattac gccgcgagcg cgtaacagcg ccgttttgaa
    38161 gtcttcttca taatctttct gcaaacccgg gatcaccgca tctttcgcgg aatcacgcat
    38221 tttcaggtga tagatcagaa tgtcatccgt caaatccgcc agttcgccgt caaaacctga
    38281 ctctttcgcc agtttctgca aaaattgcaa caggttaagg tctggctctt tttgccaggc
    38341 tggctggagg agttcaataa cttcattcag acgtttacat ttcatagtaa tgctccttac
    38401 tcttgagaca gacacgttag cagggtcaat cccacaataa aagaggcgat atcggtgaat
    38461 ctgatgacga cgataacagg cgttgtgctg gcaggcggta aagccagacg aatgggcggc
    38521 gtagataaag gattgctgga cttaaatggc aaaccgctgt ggcaacatgt cgctgacgcg
    38581 cttatgacgc agctctctca cgtcgtggtt aatgctaatc gtcatcagga aatctatcag
    38641 gcaggcggtc tgaaagtgat tgaagattca ctggcggatt acccaggccc tctggcaggg
    38701 atgctttcag taatgcagca ggaagctggt gagtggtttt tgttttgtcc gtgcgatacg
    38761 ccttacattc cccatgattt agccgcccgg cttaatcatc agcgcaaaga tgcgcctgtc
    38821 gtgtgggtcc atgacggtga acgcgatcac ccgactattg ctatggtaaa ccgcgctatt
    38881 gagcctttat tactagaata tctgcaagca ggagaacgcc gggtaatggc atttatgcgt
    38941 ctggctggcg gtcatgcggt tgatttcagc gatcataaag atgcatttgt taacgtgaat
    39001 acgccagagg agcttgcccg atggcaggaa aaacgatgat accgttactc gcctttgccg
    39061 cgtggagtgg taccggaaaa actacgcttt tgaaaaaact gatcccggca ttatgcgcca
    39121 gagggatccg tccagggctg attaagcata cgcaccatga tatggatgtt gataagccag
    39181 gcaaagatag ctatgagctg cgcaaggctg gcgcggcaca aaccatcgtt gccagccagc
    39241 aacgatgggc cttgatgacg gaaacaccag acgaagaaga gctggatcta cagtttctcg
    39301 caagtcgaat ggatacctca aagctggatt tgattctggt cgaagggttt aagcatgaag
    39361 agatcgcaaa gattgtgctg tttcgcgatg gagccggaca tcgacctgaa gaattagtga
    39421 tagacaggca tgttattgct gtagccagtg atgtgccgct taatcttgat gtcgcattac
    39481 tggatattaa tgatgttgag gggctggctg attttgtggt ggagtggatg caaaatcagg
    39541 acggataagc aagagtaggc tggagca
//
LOCUS       DABSYT020000032        39432 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.032, whole genome shotgun sequence.
ACCESSION   DABSYT020000032 DABSYT020000000
VERSION     DABSYT020000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.032"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(159..974)
                     /gene="djlA"
                     /locus_tag="ID725_RS10455"
                     /old_locus_tag="pgaptmp_002091"
     CDS             complement(159..974)
                     /gene="djlA"
                     /locus_tag="ID725_RS10455"
                     /old_locus_tag="pgaptmp_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGV7850694.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1229..3583
                     /gene="lptD"
                     /locus_tag="ID725_RS10460"
                     /old_locus_tag="pgaptmp_002092"
     CDS             1229..3583
                     /gene="lptD"
                     /locus_tag="ID725_RS10460"
                     /old_locus_tag="pgaptmp_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGV7850695.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            3636..4922
                     /gene="surA"
                     /locus_tag="ID725_RS10465"
                     /old_locus_tag="pgaptmp_002093"
     CDS             3636..4922
                     /gene="surA"
                     /locus_tag="ID725_RS10465"
                     /old_locus_tag="pgaptmp_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGV7850696.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            4922..5911
                     /gene="pdxA"
                     /locus_tag="ID725_RS10470"
                     /old_locus_tag="pgaptmp_002094"
     CDS             4922..5911
                     /gene="pdxA"
                     /locus_tag="ID725_RS10470"
                     /old_locus_tag="pgaptmp_002094"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGV7850697.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            5908..6729
                     /gene="rsmA"
                     /locus_tag="ID725_RS10475"
                     /old_locus_tag="pgaptmp_002095"
     CDS             5908..6729
                     /gene="rsmA"
                     /locus_tag="ID725_RS10475"
                     /old_locus_tag="pgaptmp_002095"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGV7850698.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            6732..7109
                     /gene="apaG"
                     /locus_tag="ID725_RS10480"
                     /old_locus_tag="pgaptmp_002096"
     CDS             6732..7109
                     /gene="apaG"
                     /locus_tag="ID725_RS10480"
                     /old_locus_tag="pgaptmp_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGV7850699.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7116..7964
                     /gene="apaH"
                     /locus_tag="ID725_RS10485"
                     /old_locus_tag="pgaptmp_002097"
     CDS             7116..7964
                     /gene="apaH"
                     /locus_tag="ID725_RS10485"
                     /old_locus_tag="pgaptmp_002097"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGV7850700.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(7961..8275)
                     /locus_tag="ID725_RS10490"
                     /old_locus_tag="pgaptmp_002098"
     CDS             complement(7961..8275)
                     /locus_tag="ID725_RS10490"
                     /old_locus_tag="pgaptmp_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HGV7850701.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(8278..8511)
                     /locus_tag="ID725_RS10495"
                     /old_locus_tag="pgaptmp_002099"
     CDS             complement(8278..8511)
                     /locus_tag="ID725_RS10495"
                     /old_locus_tag="pgaptmp_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HGV7850702.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(8597..9076)
                     /gene="folA"
                     /locus_tag="ID725_RS10500"
                     /old_locus_tag="pgaptmp_002100"
     CDS             complement(8597..9076)
                     /gene="folA"
                     /locus_tag="ID725_RS10500"
                     /old_locus_tag="pgaptmp_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGV7850703.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(9268..11130)
                     /gene="kefC"
                     /locus_tag="ID725_RS10505"
                     /old_locus_tag="pgaptmp_002101"
     CDS             complement(9268..11130)
                     /gene="kefC"
                     /locus_tag="ID725_RS10505"
                     /old_locus_tag="pgaptmp_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGV7850704.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11123..11653)
                     /gene="kefF"
                     /locus_tag="ID725_RS10510"
                     /old_locus_tag="pgaptmp_002102"
     CDS             complement(11123..11653)
                     /gene="kefF"
                     /locus_tag="ID725_RS10510"
                     /old_locus_tag="pgaptmp_002102"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGV7850705.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(11761..13092)
                     /gene="yaaU"
                     /locus_tag="ID725_RS10515"
                     /old_locus_tag="pgaptmp_002103"
     CDS             complement(11761..13092)
                     /gene="yaaU"
                     /locus_tag="ID725_RS10515"
                     /old_locus_tag="pgaptmp_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850706.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13150..13437)
                     /gene="fixX"
                     /locus_tag="ID725_RS10520"
                     /old_locus_tag="pgaptmp_002104"
     CDS             complement(13150..13437)
                     /gene="fixX"
                     /locus_tag="ID725_RS10520"
                     /old_locus_tag="pgaptmp_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGV7850707.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(13434..14720)
                     /gene="fixC"
                     /locus_tag="ID725_RS10525"
                     /old_locus_tag="pgaptmp_002105"
     CDS             complement(13434..14720)
                     /gene="fixC"
                     /locus_tag="ID725_RS10525"
                     /old_locus_tag="pgaptmp_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGV7850708.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(14771..15712)
                     /gene="fixB"
                     /locus_tag="ID725_RS10530"
                     /old_locus_tag="pgaptmp_002106"
     CDS             complement(14771..15712)
                     /gene="fixB"
                     /locus_tag="ID725_RS10530"
                     /old_locus_tag="pgaptmp_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGV7850709.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(15727..16497)
                     /gene="fixA"
                     /locus_tag="ID725_RS10535"
                     /old_locus_tag="pgaptmp_002107"
     CDS             complement(15727..16497)
                     /gene="fixA"
                     /locus_tag="ID725_RS10535"
                     /old_locus_tag="pgaptmp_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGV7850710.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            16971..18485
                     /gene="caiT"
                     /locus_tag="ID725_RS10540"
                     /old_locus_tag="pgaptmp_002108"
     CDS             16971..18485
                     /gene="caiT"
                     /locus_tag="ID725_RS10540"
                     /old_locus_tag="pgaptmp_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGV7850711.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            18516..19658
                     /gene="caiA"
                     /locus_tag="ID725_RS10545"
                     /old_locus_tag="pgaptmp_002109"
     CDS             18516..19658
                     /gene="caiA"
                     /locus_tag="ID725_RS10545"
                     /old_locus_tag="pgaptmp_002109"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGV7850712.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            19787..21004
                     /gene="caiB"
                     /locus_tag="ID725_RS10550"
                     /old_locus_tag="pgaptmp_002110"
     CDS             19787..21004
                     /gene="caiB"
                     /locus_tag="ID725_RS10550"
                     /old_locus_tag="pgaptmp_002110"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGV7850713.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            21078..22631
                     /gene="caiC"
                     /locus_tag="ID725_RS10555"
                     /old_locus_tag="pgaptmp_002111"
     CDS             21078..22631
                     /gene="caiC"
                     /locus_tag="ID725_RS10555"
                     /old_locus_tag="pgaptmp_002111"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGV7850714.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            22740..23525
                     /gene="caiD"
                     /locus_tag="ID725_RS10560"
                     /old_locus_tag="pgaptmp_002112"
     CDS             22740..23525
                     /gene="caiD"
                     /locus_tag="ID725_RS10560"
                     /old_locus_tag="pgaptmp_002112"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGV7850715.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            23531..24121
                     /gene="caiE"
                     /locus_tag="ID725_RS10565"
                     /old_locus_tag="pgaptmp_002113"
     CDS             23531..24121
                     /gene="caiE"
                     /locus_tag="ID725_RS10565"
                     /old_locus_tag="pgaptmp_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGV7850716.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(24240..24635)
                     /gene="caiF"
                     /locus_tag="ID725_RS10570"
                     /old_locus_tag="pgaptmp_002114"
     CDS             complement(24240..24635)
                     /gene="caiF"
                     /locus_tag="ID725_RS10570"
                     /old_locus_tag="pgaptmp_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGV7850717.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(24896..28117)
                     /gene="carB"
                     /locus_tag="ID725_RS10575"
                     /old_locus_tag="pgaptmp_002115"
     CDS             complement(24896..28117)
                     /gene="carB"
                     /locus_tag="ID725_RS10575"
                     /old_locus_tag="pgaptmp_002115"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGV7850718.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(28135..29283)
                     /gene="carA"
                     /locus_tag="ID725_RS10580"
                     /old_locus_tag="pgaptmp_002116"
     CDS             complement(28135..29283)
                     /gene="carA"
                     /locus_tag="ID725_RS10580"
                     /old_locus_tag="pgaptmp_002116"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGV7850719.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(29739..30560)
                     /gene="dapB"
                     /locus_tag="ID725_RS10585"
                     /old_locus_tag="pgaptmp_002117"
     CDS             complement(29739..30560)
                     /gene="dapB"
                     /locus_tag="ID725_RS10585"
                     /old_locus_tag="pgaptmp_002117"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGV7850720.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
     gene            complement(30727..31641)
                     /gene="rihC"
                     /locus_tag="ID725_RS10590"
                     /old_locus_tag="pgaptmp_002118"
     CDS             complement(30727..31641)
                     /gene="rihC"
                     /locus_tag="ID725_RS10590"
                     /old_locus_tag="pgaptmp_002118"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGV7850721.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(31707..32657)
                     /gene="ispH"
                     /locus_tag="ID725_RS10595"
                     /old_locus_tag="pgaptmp_002119"
                     /gene_synonym="lytB"
     CDS             complement(31707..32657)
                     /gene="ispH"
                     /locus_tag="ID725_RS10595"
                     /old_locus_tag="pgaptmp_002119"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGV7850722.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(32659..33108)
                     /gene="fkpB"
                     /locus_tag="ID725_RS10600"
                     /old_locus_tag="pgaptmp_002120"
     CDS             complement(32659..33108)
                     /gene="fkpB"
                     /locus_tag="ID725_RS10600"
                     /old_locus_tag="pgaptmp_002120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGV7850723.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(33196..33690)
                     /gene="lspA"
                     /locus_tag="ID725_RS10605"
                     /old_locus_tag="pgaptmp_002121"
     CDS             complement(33196..33690)
                     /gene="lspA"
                     /locus_tag="ID725_RS10605"
                     /old_locus_tag="pgaptmp_002121"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGV7850724.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(33690..36506)
                     /gene="ileS"
                     /locus_tag="ID725_RS10610"
                     /old_locus_tag="pgaptmp_002122"
     CDS             complement(33690..36506)
                     /gene="ileS"
                     /locus_tag="ID725_RS10610"
                     /old_locus_tag="pgaptmp_002122"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGV7850725.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(36549..37490)
                     /gene="ribF"
                     /locus_tag="ID725_RS10615"
                     /old_locus_tag="pgaptmp_002123"
     CDS             complement(36549..37490)
                     /gene="ribF"
                     /locus_tag="ID725_RS10615"
                     /old_locus_tag="pgaptmp_002123"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGV7850726.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(37498..37722)
                     /gene="yaaY"
                     /locus_tag="ID725_RS10620"
                     /old_locus_tag="pgaptmp_002124"
     CDS             complement(37498..37722)
                     /gene="yaaY"
                     /locus_tag="ID725_RS10620"
                     /old_locus_tag="pgaptmp_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGV7850727.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            37825..38088
                     /gene="rpsT"
                     /locus_tag="ID725_RS10625"
                     /old_locus_tag="pgaptmp_002125"
     CDS             37825..38088
                     /gene="rpsT"
                     /locus_tag="ID725_RS10625"
                     /old_locus_tag="pgaptmp_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGV7850728.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            38558..>39432
                     /gene="espX1"
                     /locus_tag="ID725_RS10630"
                     /old_locus_tag="pgaptmp_002126"
     CDS             38558..>39432
                     /gene="espX1"
                     /locus_tag="ID725_RS10630"
                     /old_locus_tag="pgaptmp_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HGV7850729.1"
                     /translation="MTDGISTSPHCLYKSNIVDDVIINKTRQNELVKVFCEYKTEFLI
                     LFDDFFRSQDLPKPSPVLHHFFQYTHLRDAHFYRCKLIEHTVQFSFFKHKGITLLRLD
                     VFDDRTSECLSEEIKIYQECHEKFIKFLKANFNQEIYPELYTPEIFYEACRNLQSFYD
                     HQETSQNAKYSAIVKKKSYFNKEIRNLIKKNIYPELYNEQCNKIPASSTDDNQKITWQ
                     NFKTSNAAYSQLCEKLSLLKSSPSRLIEKSAYCSNENMITDKFDVVFSYCGDNVKEFI
                     LLLPYNKSLEMHELN"
BASE COUNT         9685 a        10628 c         9899 g         9220 t
ORIGIN      
        1 accttaagca gggtcaacgc gataaaacca acagaaaccc tcccgtaaac cgtatattga
       61 gcctgtccac cattctgagt aaacgccttt tctcaaagaa gtgaccgcca gggcggccac
      121 cttttatcgt gggcagttac tcagcatcat ttacagggtc atttaaaccc tttctgctgc
      181 ttaatcagct cataggcctg ctgaatttcc tgcgctttct gcttcgccat ctccatcatc
      241 tcaggcggca aacctttcgc caccagctta tcgggatggt gttcactcat cagcttacgg
      301 taggcacgtt tgatggtggt cgcatcatcc gtcggcttca cgcccagcac attacaggca
      361 tcttccagcg ttgggccacg ctgcgcttgc tgccagttac caccgccaga ttgctgctga
      421 taaccgccgc caaactgtgc accgccctgc atcatgcgca aaaactggtc aaactgggcg
      481 cgggagatcc ctaattcttc agcaatgaca tacagcaccg cccgttcatt cgggtgcagt
      541 gaaccatcag caaacgccgc ctgaatctgg atctccagaa acatacgaat taagtcaaaa
      601 cgaccaaagc agacactgcg aaactggcgc atcttttcgc gcagcgggta attgtctgat
      661 tttcccaccc ggaacgcatt ttgcgccgca gtacgggaag cgccatgaag attcattcgg
      721 tccatcaact ggctggcgat atgaatatca gcctccgtga cgcgaccttt ggatttggtt
      781 aaatgcccca tcacttcaaa agtggtggca aaaaacagcg cctgacgctc acgctggttg
      841 gcgaaccacg ccattttacg gctacgggct ttatcaaaca tatggccaat taacaggcct
      901 aacactacgc cccaaaagcc gccgcccatc agtaaggcca cggccacgcc aatgattttt
      961 ccccaatact gcatatattc cccaaatcga cacacggata tcagggctat ctcccacaat
     1021 ataaaggtgc ttttaccgtt ttccggctgc ggtcaattgt gggacatcgc ctataatttg
     1081 cattatcata cctgtcattc aatgccgtgc ctaacaccac tgacgctatt cggacaggat
     1141 taacactagc gtagagatga cgagtacgtt agtctctgac cgtttgtcac gcgcaacgtt
     1201 accgatgatg gaacaataaa atcaacgtat gaaaaaacgt atccccactc tcctggccac
     1261 catgattgcc accgcccttt atagtcaaca gggactggca gccgacctcg cctcacagtg
     1321 catgttgggc gtgccaagct atgaccgtcc tctggtacag ggcgatacca atgacttacc
     1381 cgtgactatc aatgctaacc acgcgaaagg ggactacccg gatgacgccg tgtttactgg
     1441 cagcgtggat atcatgcagg gtaacagccg tctgcaggcc gacgaagtgc agctccatca
     1501 aaaagaggca ccaggacaac cggagccggt acgtaccgtt gatgcgctcg gtaatgtcca
     1561 ttacgacgat aaccaggtga tcctcaaagg gccgaaaggc tgggcgaatc tgaacaccaa
     1621 agataccaac gtctgggaag gtgattacca gatggtgggt cgccagggtc gcggtaaagc
     1681 ggacctgatg aaacaacgtg gcgaaaaccg ctataccatt ctggataacg gtagctttac
     1741 ctcctgtctg ccgggttctg acacctggag cgtggtaggt agcgaaatta ttcatgaccg
     1801 cgaagaacaa gttgcggaga tctggaacgc ccgctttaag gtgggtccgg tatcgatctt
     1861 ttatagcccc tatttgcagt tgccggtggg tgacaaacgt cgctctggtt tcttgatccc
     1921 gaacgccaag tacaccacca ccaactactt tgagttctac ctgccatatt actggaacat
     1981 cgcgccaaat atggatgcca ccatcacgcc gcattatatg catcgtcgtg gcaacatcat
     2041 gtgggagaac gaattccgct acctctccca ggcgggcgct ggcttgatgg aactggacta
     2101 tctgccttca gataaagtct atgaagatga acacccgaac gatgacagtt cacgtcgttg
     2161 gttattctac tggaaccact ccggggtcat ggatcaggtg tggcgtttca acgtcgacta
     2221 caccaaggtc agcgatccta gctacttcaa tgatttcgat aacaagtacg gttccagtac
     2281 tgacggctac gcaacgcaaa aattcagcgt tggctatgcg gtgcaaaact tcaatgccac
     2341 cgtttcaacc aagcagttcc aggttttcag cgaacagaac accagtagct actcggcaga
     2401 gccgcagtta gacgttaatt actaccagaa tgatgttggt ccgtttgata cgcgtattta
     2461 cggccaggca gtgcactttg ttaacaccag agacgacatg cctgaagcaa cccgtgttca
     2521 cctggaaccg accatcaatt tgccgctctc taataactgg ggcagcatca ataccgaagc
     2581 gaagttgctg gcaacccatt atcagcaaac caatcttgac tggtataact ccagaaacac
     2641 gaccaagctg gacgaatccg ttaaccgcgt aatgccgcaa ttcaaagttg acggcaaaat
     2701 ggtctttgaa cgcgatatgg aaatgctggc tccgggttat acccaaacgc tggaaccgcg
     2761 cgcgcagtat ttgtacgtgc cgtatcgcga tcagagcgac atctataact acgactcgtc
     2821 tctgctgcaa tctgactact ctggcctgtt ccgggaccgg acttacggcg gtcttgaccg
     2881 tattgcctcc gctaaccagg tgacgaccgg tgtcacatct cgcatatatg atgatgctgc
     2941 cgttgaacgt tttaatattt ccgttggtca aatctactat ttcacggagt ctcgcactgg
     3001 cgatgacaac ataacatggg agaatgacga caaaacgggt tcactggtgt gggcaggcga
     3061 tacttactgg cgtatctccg agcgttgggg attgcgtggc gggattcagt acgatacacg
     3121 tctggataac gtagcgacca gtaactccag cattgaatac cgtcgggatg aagaccgtct
     3181 ggtacagctg aattaccgtt acgccagccc ggaatatatt caggctacgc tgcctaagta
     3241 ctattccact gctgagcaat ataagaatgg tatttcgcag gtaggtgctg tcgccagctg
     3301 gccaattgcc gatcgttggt ccattgttgg ggcctactac tacgacacca atgcgaacaa
     3361 gcaagccgac tctatgttag gtgtgcaata cagctcctgc tgctatgcaa ttcgcgtcgg
     3421 ttacgagcgg aagctgaacg gttgggataa cgataaacaa catgcggtat atgacaacgc
     3481 aatcggcttt aacatcgaac ttcgcggcct gagctccaac tacggtctgg gtacgcaaga
     3541 gatgctgcgt tcgaacattc tgccgtatca aaacactttg tgatttgttg atttaccacg
     3601 taatccgcag tgcggttaat tgaaatggaa aaagtatgaa gaactggaaa acgctgcttc
     3661 tcggtatcgc catgatcgcg aataccagtt tcgctgcccc ccaggtagtc gataaagtcg
     3721 cagccgtcgt caataacggc gtcgtgctgg aaagcgacgt tgatggatta atgcagtcgg
     3781 taaaactgaa cgctgctcag gcaaggcagc aacttcctga tgacgcgacg ctgcgccacc
     3841 aaatcatgga acgtttgatc atggatcaaa tcatcctgca gatggggcag aaaatgggag
     3901 tgaaaatctc cgatgagcag ctggatcagg cgattgctaa catcgcgaaa cagaacaaca
     3961 tgacgctgga tcagatgcgc agccgtctgg cttacgatgg tctgaactac aacacctatc
     4021 gtaaccagat ccgcaaagag atgattatct ctgaagtgcg taacaacgag gtgcgtcgtc
     4081 gcatcaccat cctgccgcag gaagtcgaat ccctggcgca gcaggtgggt aaccaaaacg
     4141 acgccagcac tgagctgaac ctgagccaca tcctgatccc gctgccggaa aacccgacct
     4201 ctgatcaggt gaacgaagcg gaaagccagg cgcgcgccat tgtcgatcag gcgcgtaacg
     4261 gcgctgattt cggtaagctg gcgattgctc attctgccga ccagcaggcg ctgaacggcg
     4321 gccagatggg ctggggccgt attcaggagt tgcccgggat cttcgcccag gcattaagca
     4381 ccgcgaagaa aggcgacatt gttggcccga ttcgttccgg cgttggcttc catatactga
     4441 aagttaacga cctgcgcggc gaaagcaaaa atatctcggt gaccgaagtt catgctcgcc
     4501 atattctgct gaaaccgtcg ccgatcatga ctgacgaaca ggctcgtgtg aaactggaac
     4561 agattgctgc tgatatcaag agtggtaaaa cgacttttgc tgccgcagcg aaagagttct
     4621 ctcaggatcc aggctctgct aaccagggcg gtgatctcgg ctgggctaca ccagatattt
     4681 tcgatccggc cttccgtgac gccctgaccc gcctgaacaa aggtcaaatg agtgcaccgg
     4741 ttcactcttc attcggctgg catttaatcg aactgctgga tacccgtaat gtcgataaaa
     4801 ccgacgctgc gcagaaagat cgtgcatacc gcatgctgat gaaccgtaag ttctcggaag
     4861 aagcagcaag ctggatgcag gaacaacgtg ccagcgccta cgttaaaatc ctgagcaact
     4921 aatggttaaa acccaacgtg ttgtgatcac tcccggcgag cccgccggga ttggcccgga
     4981 cttagttgtc cagcttgcac agcgtgagtg gccggtcgaa ctggttgttt gtgccgatgc
     5041 cactctcctt accgaccggg cagcgatgct cggtttgccg ctcaccctcc gcccttattc
     5101 ccccaactcc cctgcacaac cgcaaactac gggcacatta acgctacttc ctgtcgcgct
     5161 acgtgaatct gtcactgcgg ggcagttagc gattgaaaat gggcattacg tggtggagac
     5221 gctggcgcga gcgtgtgatg gctgtctgaa cggtgaattt gctgcgctga tcacaggccc
     5281 cgtgcataaa ggcgtcatta acgacgcagg cattccgttt accggtcata ccgagttttt
     5341 cgaagagcgt tcgcaggcga aaaaagtggt gatgatgctg gcgaccgaag aacttcgcgt
     5401 ggcgctggca acgacgcatt taccgctgcg cgatatcgca gatgctatca cccctgcact
     5461 tttgcacgaa gtgattgcta ttttgcatca cgatttgcgt acaaaatttg gtattgccga
     5521 accgcgcatt ctggtctgcg ggctgaatcc gcacgcgggc gaaggcggtc atatgggtac
     5581 ggaagagata gacaccatta ttcctgtgct cgacgagctg cgggcgcagg ggatgaaact
     5641 caacgggccg ctgcctgccg ataccctgtt tcagccgaaa tatctcgata acgccgacgc
     5701 cgtgctggcg atgtaccacg atcagggtct tcccgtgcta aaataccagg gcttcgggcg
     5761 cggtgtgaac attacgctgg gcctgccctt tattcgcaca tcagtggacc acggcaccgc
     5821 acttgaactg gcgggacgtg gcgaagccga tgtcggcagt tttattacgg cgcttaatct
     5881 cgccatcaaa atgattgtta acacccaatg aataatcgag tccaccaggg ccacttagcc
     5941 cgtaaacgct tcgggcaaaa ctttctcaac gatcagttcg tgatcgacag cattgtttct
     6001 gccattaacc cgcaaaaggg ccaggcaatg gtcgaaatcg gccccggtct ggcggcattg
     6061 accgagccgg tcggcgaacg tctggaccag ctgacggtca tcgaacttga ccgcgatctg
     6121 gcggcacgtc tgcaaacgca tccattctta ggcccgaaac tgacgattta tcagcaggat
     6181 gcgatgacct ttaactttgg cgaactggcc gagaaaatgg gtcagccgct gcgtgttttc
     6241 ggcaacctgc cttataacat ctccacgccg ttgatgttcc atctgtttag ctatactgat
     6301 gccattgccg acatgcactt tatgttgcaa aaagaggtgg tgaatcgtct ggttgcagga
     6361 ccgaacagca aagcgtatgg tcgattaagc gtcatggcgc aatactattg caacgtgatc
     6421 ccggtactgg aagttccgcc gtcagccttt acaccaccac ccaaagtgga ttccgccgtc
     6481 gtgcgcctgg ttcctcatgc aacgatgcct cacccggtta aagatgttcg tgtgttgagc
     6541 cgcatcacca ccgaagcctt taaccagcgt cgtaaaacca ttcgtaacag cctcggcaac
     6601 ctgtttagcg tcgaggtgtt aacgggaatg gggatcgacc cggcgatgcg agcggaaaat
     6661 atctctgtcg cgcaatattg ccagatggcg aactatctgg cggagaacgc gccttttcag
     6721 gagagttaac gatgatcaat tcgccccgag tgtgtattca ggttcaaagc gtctacattg
     6781 aggcacaatc ttcacctgat aatgaacgtt acgtttttgc ttataccgta accatacgca
     6841 atctggggcg agcgccagtg cagttgttgg ggcgttactg gctgatcacc aatggcaatg
     6901 gccgtgaaac cgaagtccag ggcgaaggag tggttggcgt ccagccactt atcgcgcctg
     6961 gcgaagagta ccagtacacc agcggtgcaa tcattgaaac cccgctgggc accatgcagg
     7021 gtcactacga aatgatcgat gaaaatggcg tccctttcag catcgacatt cccgtattcc
     7081 gactcgccgt tcccacactc attcattaaa agaatatggc gacatacctt attggcgacg
     7141 ttcatggttg ttacgatgaa ctgatcgcat tgctgcataa agtagaattt acccctggga
     7201 aagataccct ctggctgacg ggcgatctgg tcgcgcgcgg cccgggttcg ctggatgttc
     7261 tgcgctatgt gaaatcctta ggcgacagcg tacgtctggt gctgggcaat cacgatctgc
     7321 atctgctggc agtatttgcc gggatcagcc gcaataaacc gaaagatcgc ctgacaccgc
     7381 tgctggaagc gccggatgcc gacgagctgc ttaactggct gcgtcgccag cctctgctgc
     7441 aaatcgacga agagaaaaag ctggtgatgg cccacgcagg gatcacgccg cagtgggatc
     7501 tgcagaccgc caaagagtgc gcgcgcgatg tagaagcggt gctgtcgagt gactcctatc
     7561 ccttctttct cgacgctatg tacggcgata tgccaaataa ctggtcaccg gaattgcggg
     7621 ggctgggaag actgcggttt atcaccaacg cctttacccg tatgcgtttt tgcttcccga
     7681 acggtcaact ggatatgtac agcaaagaat cgccggaaga ggcccctgcc ccactgaaac
     7741 cgtggtttgc gattcctgga cctgtcgctg aagaatacag catcgccttt ggtcactggg
     7801 catcgctgga aggcaaaggt acgccggaag gtatttacgc gctggatacc ggctgctgct
     7861 ggggtgggtc attaacctgc ctgcgctggg aagataaaca gtattttgtc cagccgtcga
     7921 accggcataa ggatttgggt gagggagagg cggtcgcgtc ttaaatcccg tcgagcataa
     7981 aatctaatgc tcctttaatc aacgttcggt gcgcgctggc atcgcaaaat ttcgttccca
     8041 gcgcgttaac tggaacagtt gcagtctcat gcgtcatgag cacatattct ttgccgtcta
     8101 ccagtaaaag gattgggtta agccgttctg gtgggcgaat acggctaaat cgttcaatag
     8161 gtgttaatgg aataacaata cggcgattaa tcacaccaat aatgtcgctg gtaacatcga
     8221 taacaaaggg aaacgcggcg ttcctgctgc gactgcggta taccgtaaat tgcatcgcta
     8281 gaagctccgg tactcatcag agaaacatcc ggtttcatca gccaattgat ttaacgcagc
     8341 gatagcttca gcgttctctt cacgccaacg tgttgctgca tgttttttaa gttcagcatt
     8401 gagcgcaacc gtaagggtgg cgctcatatt taagcctgca tcacgagcgc gattgactaa
     8461 ctcacggtcg acggtgacgg tcacactttg tgtggtacgt tttgcagtca tgatatcgtg
     8521 ctcctgtaca atttatacgt ctagtataca cggatatctg cacgtatcag tgatgccgga
     8581 attctaatat acaaaattac cgccgctcca gaatctcaaa acaatagctg tgagagttct
     8641 gcgcatcagc gtcgtggaat tcgctgaata ccgattccca gtcatccggc tcgtaatccg
     8701 gaaaatgggt gtcgccttcc acttctgcgt cgatatgcgt caggtacagt ttttgcgctt
     8761 tcggcaggaa ctgctcataa acgcgaccgc cgccaatcac catgatttct ggtacgtcac
     8821 cacacgccgc gatggcttca tccaccgact tcacccacgt tacgcgatcg tccgtacccg
     8881 gttgactgct gaggataata tttttgcgtc ctggcaacgg acgaccgatg gattccctgg
     8941 tatggcggcc cataattacg ggtttattta aggtgttgcg tttaaaccag gcgagatcgg
     9001 caggcaggtt ccacggcatg gcgttttcca tgccgataac gcgatctacc gctaacgccg
     9061 caatcagact gatcattgag atttcccgat aaaaaaaatt gtcgccacta tacgtaaagc
     9121 gtaaaccgtc gtcgactggt gcgaggatga tgttgaggaa aattttatat tctgctggcg
     9181 agtccacgct ctctccctgg actcgccgca ttacaatgaa acaaaaacaa acagttagct
     9241 gtaaagtgtg atttacgtca ctctttatta ggatgagggt ttcgtttccg gttcatccgc
     9301 catgttgccg gtatgtttac cttcttctgt tccctgccag ccatgtcgtt gaattaatga
     9361 cagatgttcg cggtcctcgg tgatgatctc acttaacatc gcgctggtgc gtttatagac
     9421 cgccgcgcgg gctttggtgt cattctcaac cattgccatc tcttccacca tctgaatatt
     9481 aaagcggcgg aacacatcgg cacgttctcg cgcttcatac ggccccagac ctaaactttc
     9541 cagcgccaga cgcccggttt tcagcgcacc ttcaaaggtt tcacgctctg gcttttcaac
     9601 gcctgcctga cgcaaacaaa tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa
     9661 atgcgggaaa tgttctttca ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat
     9721 ggcgttaatc agcacttccg ctttcgccgc tcccgcagat tccagtaaat ccatccgcgt
     9781 ggcatcgcca taaaacactt tcataccaaa tttacgcagc gtttcgatat ggtccggatc
     9841 gtgatcgagc accaccattt tcaccccgct ggagagcagt aaacgtccgg tgatctgccc
     9901 gaaacgaccg aatccggcaa tgattacccg cggctgttct tcgtcgatct catcggcttc
     9961 acgcgcctct tcagtagaag attgctcaag gcgattgagg atcaccagca gaatcggcgt
    10021 tgccgccatc gacagcgcca ccgccagggt cagcgatttc gcccactccg gctccagcac
    10081 attcgccatc tgcgccgcgc caaataccac aaaggcaaac tcactgccct gccctaacaa
    10141 caccgcaaac cagcgacgct gtttatttgg cacctgcaac ggtcgggcaa tcagccacag
    10201 catggcgatt ttgatgatga ggaaaccgag cagcaaaatg acaatgcgca atgggttttc
    10261 aatcagcgtg ccaaagtcta tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa
    10321 acctttaaag ggttcgatat cgctttccag cgcatgacgg tattccgagc ttgccagcag
    10381 tacgcccgcc agaaacgcgc ccatcgccat cgacaagccg acctcttcca gcagcaaacc
    10441 aaagccaaac acaaggaata acgccacggc actaaacact tcccgcaagc cagagcgggc
    10501 aacaaaacgc agcgccggac gcgtgacata gcgccccagc agtaccacca gcaccagcgc
    10561 gcccgccact tttaacgccg agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc
    10621 cgccagtagc gggatcatcg ccaccagcgg gattgccgcg atatcctgga acagcagcac
    10681 cgcaaaggca ctgcggccca tttgcgtcac catcagattg cgttcattca tcgcctgcat
    10741 ggcaatcgcc gtagaggaga gcgccagcgt catgccgatc aattccgcga cctgccagcg
    10801 cagcccaagt aacatacaga acagccccag caggccgccg caaatcacca tctgcaatgc
    10861 gccaccgcca aacactgccg cacgcagctt ccacagcctt tgtggatcga gttcgaggcc
    10921 gataataaac agcatcagca ccacgccaat ctcggcaaag tgcagaatag attcggcatc
    10981 ggtcaccagt cgcagccccc acgggccaat aatgcagccg gcgatcaggt agccaagtac
    11041 cgatcccaga ccaagacgta ctgcaatggg tacaatcagc gccgccgaac cgagataaat
    11101 cagcgcctga accagcgtat ggctatccat gatgggcctc ctgccattcc agcagacgtt
    11161 gcttatagtg acgcgcctgc ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca
    11221 tggcaaacgg tggcagccag ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg
    11281 acagcacatc aaagcccgga tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg
    11341 tcaccgccca cagcaaatgt ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc
    11401 cgtgcgagaa gactttatcg atccaaagtt taaggagcgg aggaatgctg taccactgca
    11461 tcgggtgctg ccagacgatc agatcggcgc gagacagcgc ctcctgctcg gcggcaatat
    11521 cgatattgaa gtcaggatag agttgataaa gagagcgaat ttcgacgcct tccagcgtcc
    11581 ttgcctgttc aaacatccgt ttattcgcat gggaatgatg cggatacgga tgcgcataaa
    11641 ttataagaat cataggtagc ccgcgtgttt acgcctttgt tttcccacaa agtgtagtca
    11701 tatattcacg cggcttatag tcaataatat tgattcagaa aagagataaa tctgaacaaa
    11761 ttaccccatt ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg acatccctcg
    11821 agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat
    11881 tagcatcgtg ttactgatac cgtaattatt gataaagatc ggtagtgccc aggtcgaaac
    11941 aatggtgcca atacgactta aggacataat cacgcccacg gcagaggcgc ggatatccgt
    12001 cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc
    12061 atacaccgca aaggccatca ctaccagcca gatccccata tccgggatca gccccaaaac
    12121 cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt
    12181 gtttagccac agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacgtt
    12241 ccccagtgcc gcgtttttgc caacacccaa tcccaacaaa ccaacgattt gcgggccaaa
    12301 ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac
    12361 aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc
    12421 atcgaaagcc accggttcgc caaacagttt gatcatcatc tcttcgcact cttttactcg
    12481 ccctttgcgt aataaccagc ggggagattc aggcagttcg aatcgaccaa tcaaaatcaa
    12541 caaacagggg atcgccgcgc tacccagcat ccagcgccag ccgccttcca catcataaag
    12601 ccagtagccg accagatcgg cacaggtcgc gccgacatac cacatggcgg cgataaagct
    12661 gatggaaaac gcccgctgac gggtactgga gaactcagtg atcatcgaag tggcgatggg
    12721 atagtctgca ccgatgacaa tgccgataag tacccgcatc accaacagtt cgacggggga
    12781 tgaaacaaac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat
    12841 tttgcgccgt ccgactttat cggaaatata accaaacagc gatgtgccaa cgaacagccc
    12901 ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg tccagtttta gcgccggcgt
    12961 cagttgctcc agcgccacgc caatcattac cagtatataa ccatccagaa acggaccacc
    13021 gcttccccac agcaaaatgc ggcggtgaat agaggaaaat ttaagatcgt caaagtttct
    13081 ggacggttgc atgggttatt cctgtttttt gcaatgagag tcagtgcggc gttacgtatc
    13141 aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt attcccactg
    13201 ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac atccggcgta
    13261 atcgaagcgc acactgccgt catcctgctt cttgtacaga cctgcggggc acgctttcac
    13321 cagcagctcc agcgcctgtt tatcggcatc ggctttcaca acaatgtgcg gatgctcttc
    13381 atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag aagtcataaa
    13441 acggtcactc ctttcatgcc atccttgatc agattgatga agcccacttt cttgccgtgg
    13501 cggaggattt tcttgcgcat cagttccggc gcactgccgt caatggtgaa caggtcacgc
    13561 gccacgccta ccgccagttc cgggtagccg ctaaacatgc gtgggttatc aaggaaggcc
    13621 ggtagtttct ggtacatacg catatcgcgc agcgggccac tctcaagatg ctgacgatat
    13681 tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac ggtttttgct
    13741 gcagcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc gaggttcata
    13801 cacattccgg cggcatcacc ggcaatcaat acgccgtcac cgaccagctc cggcagcatg
    13861 ttgatgcctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc acccgcgatc
    13921 agcggtgcaa cggccggatg ttgtttgaaa tcttccagca tttgcggcac cgattttttc
    13981 gcgtcatgca gatgatgcag accacaaacc agccccagcg acagggtgtt ttcattggta
    14041 taaagaaaac cgccgcccat caggccatcg gtgggtgatc ccgcaaatag gcaagccgcc
    14101 ccctgattac cctgcaactg aaaacggtct tcaataaccg acttcggtaa ctcgatcagt
    14161 tccttcacgc caaccgccac attcgtcggt ttaacgcgtt ttgccatccc cagcttttcg
    14221 gcaaggatgg agttcactcc atcagcaagg atcaccgttt tcgcttcaat cacatcgcca
    14281 tcggcttcta caccgacgac tttgccatcg cgctgtacga ggttatcgac gcggatcccg
    14341 gtaattaact gcgcgcccgc ttcttcggcc tgctccatca gccaggcatc aaatttactg
    14401 cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt caccgttgca gtagtccata
    14461 gtcatcgctg acttttccgt cataaacgcg agtttttcat gggtgatcag gcgttctacc
    14521 ggggcggatt cggcgaaacc aggaataatg tgttccaggc tgtgggcata gagacgcccg
    14581 ccggtgacgt tcttggcacc tgcggaattg ccacgctcga taactaacac ttgcgcccct
    14641 tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc ctgcaccgac gatgatggcg
    14701 tcaaagatat cttcggacat aagagctccc tgtcagcggc aggaccaaaa tgcagccctg
    14761 ccagagtgga tcaacgcgct aaagctgcgg tcagcgccgg aaggatcttc acggcgtcgc
    14821 caacaatgcc gtaatccgcg tactggaaga tcggcgcatt tttatcttta ttgatggcga
    14881 aaatggtttg cgacgcgtta gcgccaacca tgtgctggat ctgcccggag atccccacag
    14941 ctaggtacag ttcaggtttc agcatcaggt tggagatacc gacatagcgt tcgtgctcca
    15001 tccatttttc gttttctgcc accggacgag aacaggccaa ctccgcacct atcgccttgc
    15061 aaagctgttc tgccagcgca atgttctctt tgctgccaat accgcgaccg acgctgacca
    15121 ccaggcgggc tttgtcgaga tcgacgctgt tgctctggcg cgcctgggtt gccgtgcggg
    15181 taatcgccac agccggagcc tgccactcca cggtgtgcgt ttcgccagtg cgtgacgcgt
    15241 ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag taccgcatac ggcgtggcaa
    15301 tgcgttcttc gccaatcgcc agaccaccgt aaaccatatg tttcactgtt gctttaccgt
    15361 cctgtacgct gacggtgctg gcatcgttag acaccgccgc tttaaggcga taacccagtt
    15421 ttgccgccag taatttgccg cgacgggtgt ttggcaacag caccaggccg tctgcaccgt
    15481 gctggcgaat agtgtcagcc atgacactgg cgtaatcttc gatcatccga tcgtccggtt
    15541 tgccgtttaa tttccagaca tgattagcgc cgagctggat tgcctgtgcg ccgtcggcat
    15601 cattgaggac aaaggcattg atttgattag ctaaagcctg cgcaccgttc atcagttccg
    15661 gcagacgaga aggggtatcg ctgaataccc agacttgaga aaacgtgttc atagcatccc
    15721 ctgtaattaa atgactttgc gaagattttc agcaaatgcg gcgatctgtt cttcgccgtc
    15781 gccttcaatc acgatgcgct gacgctcgcg ctgtttcggc gcggcaacct gttgttctga
    15841 ccatgctgcc tctgcgttaa aaccgatatc cgccgccgac catacctgga ctggcttttt
    15901 cgccgcgccg agaatggctt tcatcgaagg aatttgtggg gagttgatat cagtggaaac
    15961 agcaacaacc gcaggcagtg gaatgcttaa ggtttcggtt tcatcttcca gttcgcgctc
    16021 aacggtgagg gtatctgccg tcagggagat aattttgctg acgccgttaa ctgccggaat
    16081 attgaggatt tcgcctacca gcaaaccaac ctgctgggca taaaggtcgg aagaaccatc
    16141 gccacagagg atcaaatcaa agcctgcttt ctgggcggct gcagccagtg cgctcgccgt
    16201 ttgttgcggc agtgcctgct cgaactggtc atcaatcacc acaatcagtt catccggacc
    16261 gcgcgatagc acatctttac gccctttggc gttggtcagg gctttaccgc ccacacttag
    16321 ggccgtcacc tgcgcctctg ctgcctgttg ctttagctgg caagccgctt caatagcgtt
    16381 gagatcgtat tggcttattt tggcatcggc tttactgaag tctaacgaac catcagcatt
    16441 attgaccgca atatcctgtt catcaggcac gcacttatag caagtaataa tcttcattgc
    16501 atctccagaa atcatgaagg aaacgtcgtt catatcggtg gtgttaatta cagatatcat
    16561 gccctttaat ttggaaataa aactttaatc accaatattg aaaatgtcac acgcagataa
    16621 atccaacttt caatattgtt aagttcctca ccaatattga aaacacggcg tagcaaaaag
    16681 aaattttcaa tattgtttta tggatcacca atattgaaag tcagatgcta tttttttact
    16741 cgcaacataa cataacaaac tgattaataa tcaaaattaa cgagaaaagc gccctctatt
    16801 cacccgaaac aaaaatgtga taacaatcac agaatgcagc ttattgaata cccattatga
    16861 gttagccatt aacgcgtcca tgaggttaat aataattata ttaaatgtta acaaaaataa
    16921 aacaaacggg aacgcaaaat aaatattcgt tttcacagtg gaattaactc atgaagaatg
    16981 aaaagagaaa aacgggaata gaaccgaagg ttttctttcc gccattaata atcgtcggca
    17041 tactttgttg gcttacagtc agagatctgg atgcggcgaa tgtcgttatt aatgctgtat
    17101 tcagttacgt caccaatgta tggggatggg catttgaatg gtatatggtg gtgatgcttt
    17161 tcggttggtt ctggctggtg tttggcccgt atgccaaaaa gcgtttaggt aacgaaccac
    17221 ctgaatttag taccgccagt tggatcttta tgatgttcgc ctcctgtacg tctgctgccg
    17281 tactgttctg gggatcgatt gagatctact actacatctc caccccgccg tttggcttag
    17341 aaccgaactc gacaggagcg aaagagttgg ggctggctta cagcttgttc ctctggggac
    17401 ctctgccgtg ggccacttac agcttccttt cagtcgcctt cgcttacttc ttctttgtcc
    17461 gcaaaatgga agtgattcgc cccagctcca ccctggtgcc gctggtaggt gaaaaacacg
    17521 ccaaagggtt gttcggcact atcgtcgaca acttctatct cgtcgccttg atcttcgcga
    17581 tgggtaccag tctgggcctt gccacgccgc tggtgaccga gtgtatgcaa tggttgtttg
    17641 gcattccgca taccctgcaa ctggacgcta tcatcattac ctgctggatt atcctcaacg
    17701 ccatttgcgt cgcctgcggt ctgcaaaaag gggtacgtat cgccagtgac gtgcgtagtt
    17761 acctgagctt cctgatgctg ggttgggtgt tcatcgtcag cggtgccagc ttcatcatga
    17821 actacttcac cgattcggtg gggatgttgc tgatgtatct gccgcgcatg ttgttctata
    17881 ccgatcccat cgctaaaggc ggcttcccgc agggctggac cgtgttctac tgggcatggt
    17941 gggtgattta cgccatccag atgagtatct tcctcgcccg catctcccgt ggtcgtactg
    18001 tgcgtgaact gtgcttcggc atggtgatgg gactgacagc atccacctgg atcctgtgga
    18061 ctgtactcgg tagtaacact ctgctgttga tagataaaaa catcatcaac attccaaatc
    18121 tgatcgaaca gtacggtgtg gcgcgcgcca tcatcgaaac ctgggccgct ctgccgctca
    18181 gcaccgccac catgtggggc ttcttcatcc tctgctttat tgccaccgtt acgctggtta
    18241 acgcctgctc ttataccctg gcgatgtcca cttgccgcga agtacgcgat ggtgaagaac
    18301 caccgctgct ggtgcgtatc ggttggtcaa ttctggttgg cattatcggt attgttttgc
    18361 tggcgctcgg cggcctgaaa ccgattcaaa ctgccattat cgccggagga tgcccgctgt
    18421 tcttcgtcaa cattatggtg acgctctcct ttattaaaga cgcgaaacag aactggaaag
    18481 attaattaac ccccaaaata tcaagaggtt gaaagatgga ttttaattta aatgatgagc
    18541 aggaactgtt tgtcgccggt atccgcgaac tgatggccag cgaaaactgg gaggcctatt
    18601 ttgccgagtg cgaccgtgac agcgtctacc cggaacgttt tgtcaaagca ctggcggata
    18661 tgggtatcga cagtctgctg atccctgaag agcacggtgg tctggacgcg gggtttgtta
    18721 ctctcgccgc cgtgtggatg gagctgggac gtctgggggc accaacctat gtactgtacc
    18781 agttgccggg cgggttcaat accttcctgc gcgaaggcac acaagagcag atcgacaaga
    18841 ttatggcttt ccgcggcacc ggtaagcaga tgtggaactc agcgattacc gaaccgggcg
    18901 cgggctccga cgtgggtagc ctgaaaacga cttatacccg tagaaatggt aagatttatc
    18961 ttaatggtag taagtgtttt attaccagta gcgcctacac cccgtacatc gtggtgatgg
    19021 cgcgcgacgg ggcttctccg gacaaacctg tctacaccga atggtttgtt gatatgagca
    19081 aaccgggcat caaagtgacc aaacttgaga agctcggtct gcgtatggat agctgctgtg
    19141 aaatcacctt tgacgatgtg gaactggacg agaaagacat gttcggtcgg gaaggtaacg
    19201 gctttaaccg cgtcaaagaa gagttcgacc atgaacgttt cctggtagcc ctgaccaact
    19261 acggtacggc gatgtgcgcc tttgaagatg cggcgcgcta cgccaaccag cgcgtgcagt
    19321 ttggcgaggc tattggtcgt ttccagttga ttcaggaaaa attcgcccac atggcgatca
    19381 aattaaactc catgaaaaac atgctgtatg aagcagcgtg gaaagcagac aacggcacca
    19441 tcacctctgg cgatgcagcg atgtgcaaat acttctgcgc caatgcggca tttgaagtgg
    19501 tggatagcgc aatgcaggtg ctgggcggtg tcgggattgc gggcaaccac cgcatcagcc
    19561 gcttctggcg tgacctgcgt gtagaccgcg tttccggagg atctgacgaa atgcagatcc
    19621 tgacgctggg tcgtgcggtg ctgaagcaat accgctaagt ccgtgcgctg cctgatgcga
    19681 cgctgacgcg tcttatcagg cctacgggag cacgaatgta ggccggataa ggcgtttacg
    19741 ccgcatccgg cagtcatgcg ctacataaat ttttcaggag ctaataatgg atcatctacc
    19801 catgccgaaa ttcgggccgt tggccggatt gcgcgttgtc ttctccggta tcgaaattgc
    19861 cgggccgttc gccgggcaaa tgttcgcaga atggggtgcg gaagttatct ggatcgagaa
    19921 cgtcgcctgg gccgacacca ttcgcgttca accgaactac ccgcaactct cccgccgcaa
    19981 tttgcacgcg ctgtcgttaa atattttcaa agatgaaggc cgcgaagcgt ttctgaaatt
    20041 aatggaaacc accgatatct tcatcgaagc cagtaaaggt ccggcctttg cccgtcgtgg
    20101 cattaccgat gaagtactgt ggcaacacaa cccgaaactg gttatcgctc acctgtccgg
    20161 ttttggtcag tacggcaccg aggagtacac caatcttccg gcctataaca ccatcgccca
    20221 ggccttcagt ggttacctga ttcagaacgg tgatgttgac cagccaatgc ctgccttccc
    20281 gtataccgcc gattactttt ctggcctgac cgccaccacg gcggcgctgg cagcactgca
    20341 taaagcgcgt gaaaccggta aaggcgaaag tatcgacatc gccatgtatg aagtgatgct
    20401 gcgtatgggc cagtacttca tgatggatta cttcaacggc ggcgaaatgt gcccgcgcat
    20461 gagcaaaggt aaagatccct actatgccgg ttgcggtctg tataaatgtg ccgacggcta
    20521 catcgtgatg gaactggtgg gcattaccca aattgaagag tgctttaaag atattggcct
    20581 cgcacatcta ctcggtacgc cggaaatccc ggaaggcact cagcttatcc accgtatcga
    20641 atgcccttac ggcccactgg tagaagaaaa actcgatgcc tggctggcgg cacataccat
    20701 cgcagaagtt aaagaacgct ttgccgaact gaatatcgcc tgtgccaaag tactgaccgt
    20761 accggaactg gaaagcaatc cacagtatgt tgcccgtgaa tcaatcactc agtggcaaac
    20821 gatggatggt cgcacctgca aagggccgaa catcatgccg aaattcaaaa ataaccccgg
    20881 acaaatctgg cgcggaatgc cctcacatgg catggacacg gctgccattt tgaaaaatat
    20941 cggctacagc gaaaacgaca ttcaggagtt ggtcagcaaa ggtctggcca aagttgagga
    21001 ctaaacgcgt ggcgtcagtc ggcttgcctg actagcgccc gcccaataac aaacgaacaa
    21061 aaatggatag aggtgcaatg gatatcattg gcggacaaca tctacgtcaa atgtgggacg
    21121 atctggcgga cgtttacggt cataaaacgg cgctgatttg tgaatccagc ggcggagtcg
    21181 ttaaccggta tagttatctt gagttaaatc aggagattaa ccgcacggca aacctgtttt
    21241 atacgctggg gattcgcaaa ggcgacaagg ttgcactaca tctcgacaac tgcccggaat
    21301 ttatcttttg ctggttcggg ctggcaaaaa ttggcgcgat tatggtgccg attaacgccc
    21361 gcctgttacg cgaggaaagt gcgtggatcc tgcaaaatag ccaggcgtgc ctgctggtga
    21421 ccagtgcgca attctatcct atgtatcaac agattcagca agaagatgcc actcaattgc
    21481 ggcacatttg cctgacagat gtggcacttc ccgctgatga tggcgtgagt tcgtttactc
    21541 aactgaaaaa tcaacaacct gccaccttgt gctatgcacc gccgctatcg actgacgata
    21601 cggcggaaat tctcttcacc tccggcacca cctcccgacc gaaaggtgtg gtgattaccc
    21661 attacaacct gcgcttcgct ggatattact ccgcctggca gtgtgcactg cgtgacgatg
    21721 acgtctacct gacggtaatg cctgcgtttc atatcgattg ccagtgtact gcggcgatgg
    21781 cggcgttttc tgccggggcc acctttgtgc tggtcgagaa atacagcgcc cgcgccttct
    21841 ggggacaggt acagaagtac cgcgccacca ttaccgaatg tattccgatg atgattcgta
    21901 cgttgatggt gcagccgcct tcagcgaacg atcggcaaca ccgcctgcgg gaagtgatgt
    21961 tttatctcaa cttgtcggag caggaaaaag acacattttg tgaacgcttc ggtgttcgct
    22021 tgctgacgtc ttatgggatg acggaaacca ttgtgggcat tatcggcgat cgccctggcg
    22081 ataaacgacg ctggccgtcg attggtcggg cggggttttg ctacgacgcg gagatccgcg
    22141 acgatcacaa tcgcccgctc ccggcaggtg agatcggtga aatctgtatt aaaggcgtac
    22201 cagggaaaac catcttcaaa gagtattttc tcaacccgaa agccactgca aaagtgctgg
    22261 aagctgatgg ctggctgcat accggcgata ccggataccg cgacgaagaa ggcttttttt
    22321 atttcatcga tcgccgctgc aatatgatca aacgcggtgg cgagaatgtc tcctgcgtgg
    22381 agctggaaaa tatcatcgcc acccatccaa aaattcagga tatcgtggtt gtgggtatta
    22441 aagattcgat tcgcgatgaa gccatcaaag catttgtggt gctgaatgaa ggtgaaacat
    22501 tgagcgaaga ggaatttttc cgcttctgcg aacaaaatat ggcgaaattt aaagtgccct
    22561 cttatctgga gatcagaaaa gatctgccac gtaattgctc ggggaaaata attagaaaga
    22621 atctgaaata aatgaaacgg cagggaacta ccctgccagc taataatcac gctctaaaac
    22681 aatatgcatt ttttgcaggg atgttgtcgt ccctgaaaaa gcaaaaatgg agaaaaggaa
    22741 tgagtgaatc attacatctg acccgcaatg gatcaattct ggaaattact cttgatcgcc
    22801 caaaagcgaa tgctattgat gcaaaaacca gctttgaaat gggcgaagta tttctaaatt
    22861 tccgtgacga tccgcaatta cgtgtcgcca ttattaccgg tgccggagag aaattctttt
    22921 ccgcgggctg ggatttaaaa gcggcagcag aaggcgaagc accggatgct gactttggcc
    22981 cgggtggttt tgcgggatta accgaaattt tcaatctcga caaaccggtt atcgcagctg
    23041 tgaacggcta cgcctttggc ggcggtttcg aactggcgct ggcggcagat tttattgttt
    23101 gtgccgataa cgccagcttc gccctgccgg aagccaaact gggcatcgtt cctgacagcg
    23161 gcggtgtgct gcgtctgccg aagatcctgc cgcctgccat cgtcaatgaa atggtgatga
    23221 ccggcagacg aatgggcgca gaagaggcgc tgcgttgggg gatagtcaac cgcgtggtta
    23281 gccaggcgga actgatggat aacgcccgcg aactggctca gcagctggtt aacagcgccc
    23341 cgctggcgat tgcggcgctg aaagagatct accgcaccac cagcgaaatg ccggtagaag
    23401 aatcgtatag ctatattcgc agcggcgtgt tgaaacacta tccatcggtt ctgcattcgg
    23461 aagatgccat tgaagggccg ctggcgtttg ccgagaagcg cgatccggtg tggaaaggac
    23521 gttaacaacc gtgagctatt acgcctttga ggggttaatt ccggtggttc acccgacggc
    23581 gtttgtccat cccagtgccg tcttgattgg cgatgtgatt gtgggagccg gtgtctacat
    23641 cggcccactc gcctcactgc gtggtgacta cgggcggttg atcgtgcaag cgggagccaa
    23701 tattcaggat ggctgcatta tgcatggcta caccgacact gacaccatcg ttggggaaaa
    23761 cggccatatc gggcacggag cgatcctgca tggttgtgtg attggtcgcg atgcattggt
    23821 cgggatgaac agcgtgatta tggatggcgc ggtcattggc gaagagagca ttgttgccgc
    23881 catgagcttt gtcaaagcag gctttcacgg cgagaaacgc cagttgttga tgggtacgcc
    23941 agcccgcgct gtacgcagtg ttagtgacga cgagttacac tggaaacggt tgaataccaa
    24001 agagtatcag gatcttgtcg ggcgctgtca tgcatcgtta catgaaacac agccactgag
    24061 gcaaatggag gaaaatcgcc cccgtttgca ggggacgacg gatgtgacgc ctaaacggtg
    24121 atcttgccgg atgcggcgta aacgccttat ccaactctgc tggagagcaa ccgtaggccg
    24181 gataagatgc gccagcatcg catccggcga ccaggtcgca cggtatcatc gaaatgacat
    24241 taacgacgca tactctttga caacatcatc tgccacttct gctcgcggtt aagttccgtg
    24301 agcggaacgg caggaatacg cggtgtcttt cttacgccga ctaatttttc ccgactgtta
    24361 ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca ctttaaccag tcgctgacac
    24421 tggcatcccc gcccttccag cttattaggg atcatcttaa cttcgcagct tatttctgtg
    24481 acttccgaca gaatataagt cagggtatta accgccttgc tgtgttcaat atcgaaatga
    24541 atagagatct ctcttgctat cacccaccga ttttcagcca tcatccattc ggcgattaac
    24601 aagtagagtg gtttttcaac atatccttca cacatacgaa ctccagtttc acaaattaaa
    24661 agtctggcta tgactcgttt tgaagcaatt aaacaaggcc atattaaaat tgtggatata
    24721 tcgatagtat gcaacttcac cgtcgataag tgtgagctaa atcctgctta tgtcatcaaa
    24781 aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa aacggtcctc atcagagaac
    24841 cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccatgaca cgctattatt
    24901 tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg gcattcagcg
    24961 ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta tattgcagcg
    25021 cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg gtgttgatga
    25081 tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct tcatgcacct
    25141 tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg ccgtgggtcg
    25201 catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg cgttctttat
    25261 cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag ttgctgccca
    25321 gctgcgcttt ggcaaacgct tcagcgaagg tgcggcccac gcccatgact tccccggtag
    25381 aacgcatttc tggccctaac agcgggtcaa cgcccgggaa tttattgaac ggcagcacca
    25441 cttctttcac cgagtagtac ggcgggataa cttctttggt tacgccctgc tcagccagcg
    25501 atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg gctttggaga
    25561 cgaacggaac ggtacgcgcc gcacgcgggt taacttcaat caggtagact tcgttgtttt
    25621 tcaccgcaaa ctgcacgttc atcaggccgc gcacctgcaa ttcgaaggcc agtttctgca
    25681 cctgctggcg cattacatcc tgaatttcct gacttaaggt gtacgctggc agcgaacatg
    25741 cggagtcacc ggagtgcacg cccgcctgct cgatgtgctc catgatgccg ccaatcagca
    25801 ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca aggaaatgat
    25861 ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag cgacgcaggt
    25921 cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac ggacgcacca
    25981 ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca gtaacggtgg
    26041 cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt tcacggtctt
    26101 ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg gcttccagtg
    26161 cacgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct ttcggcttct
    26221 caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag aggcgatcgg
    26281 aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg gtttcgtaac
    26341 cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg ataccctgac
    26401 cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta gacggattcg
    26461 cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca aactctgccg
    26521 cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc agcttacgga
    26581 tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag cctttgcgtt
    26641 tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc actttctctt
    26701 ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta gtcaggttga
    26761 agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag atacgctctg
    26821 cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca tccaggctca
    26881 ctttcgggtc gaatccagtc gcaccgactt ccaggccgcg cagcgctttt tgcagggatt
    26941 cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga gtggtcagac
    27001 ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttggta accacgtaat
    27061 cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc atcagttcat
    27121 cgagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa ccggtcgctt
    27181 tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca atcaggcgac
    27241 cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg ccgatttcac
    27301 gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc agcgtttggg
    27361 ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag ttttcgatag
    27421 agcagacgat gatgcagttg tcgtttttat cgcgcaccac ttccatctcg tactctttcc
    27481 agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc agaccgcggg
    27541 cgcaaatttc ttcaaactct tcgcggttat aagcgatacc gccgccgcta ccgcccatgg
    27601 taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaaccgcc agcgcttctt
    27661 ccatcgtatg tgcgataccg gaacgcgcgg tttccagacc aattttcttc atcgctacgt
    27721 cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca atcatggtga
    27781 cgccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc agcgccgtct
    27841 gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc ttacgtacca
    27901 cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg gtcatgatgg
    27961 tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg gctttacatg
    28021 cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg cccgcaccca
    28081 gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc ctgattactt
    28141 agcggtttta cggtactgct caattaactc gataaagtgg tcgaacaacg gcgcggcgtc
    28201 gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggtt tatcggtgcg
    28261 atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca ggtttgcagg
    28321 taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca ccacgttttt
    28381 ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt tgacagtctt
    28441 cgcaccgctc gccagcgcca gcagctgatg accgaggcag atgccgaaga ccggaatatc
    28501 ggtttcgagg aatttctgga tggcggtaat ggcgtaatcg cacggggccg ggtcgccagg
    28561 accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg cagaagtttg
    28621 cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga tgttgcgttt
    28681 ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt tcgcttctgg
    28741 caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg cggtggtcac
    28801 ttcttttgcc agatccatgc cattcagacc tgggaacgcg cgggcttttt ctaacgccag
    28861 cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt tctcgcgcag
    28921 taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat ggcgcttcag
    28981 gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca ggtcgcgaat
    29041 caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag tgccgacatt
    29101 gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat cagtgaggat
    29161 ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg aacctgttgc
    29221 ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg ctgacttaat
    29281 caaaacaccc tccagagaat attcactcac tttatttgca tattaattca caatgatgac
    29341 gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca ttctggagat
    29401 atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg gctcatgcat
    29461 gttttacagg cattaagcaa caaaaagatc atctaacctg ttctggaaaa cgcttgcgca
    29521 gcaagaagat tgcatgaaat aaaaacgagc aggcaaaagt ggaccaaatg gtcaaaattt
    29581 acattaagac aacaaaaata gaagacaaaa catgtaattt ttggcagtta tagaaaaatt
    29641 aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa ttaataaatt
    29701 aaatatgtta cttttgcatc atcataaaca ttttgtggtt acaaattatt gagatcaagc
    29761 acatctcgca tatcaaaaag accattttcc ttaccactca accacaaagc cgatcttacc
    29821 gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag acgctcgcca
    29881 atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac ggtggcaaaa
    29941 ccaatggtac caggcacacg ttcaccggta tggccttcac gactgtagac cgcgcaatct
    30001 ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc ggtgcctgac
    30061 ggcgcatcaa ctttatgtct atgatgtgct tcaataattt caatatcggt gtagtcaccc
    30121 atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac gctaaaattg
    30181 gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt tccggcttcg
    30241 tcaaaccccg tagtgccgat caccatccct ttgccatgct ggcgacaaaa agcgagatgg
    30301 ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc ttttatcgca
    30361 tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc accggcgtcg
    30421 ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac gccttctaat
    30481 gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc gatggcaacg
    30541 cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac cgttttcaga
    30601 gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa ttaatgataa
    30661 acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc gataacatgt
    30721 gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc cttttacatt
    30781 cagatccaat gccacctgta cattggctgg tttgcccagg caaccgtcga tatcaaccac
    30841 cgtcgtgcct gaggtaaatt cgccttgagt ttccactgcc acaaaacagg gtttgagagt
    30901 gaacaggtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc gcaagccgct
    30961 ttgcatactg ccgctacggt agtggctaaa cagggcgtga agcattttcc cggtacggtt
    31021 taactgcggc agtgtagaga gatagtcagg agttaatatt gcctgattgg tgacatccaa
    31081 accgcacatg acgatttcaa taccactgcg gaagacacag gcagcagctt ctggatcggc
    31141 agcaatatta aactcggcgt ttggcgtaca gttgccgcgt ccggcagaac cacccatgat
    31201 caccagacgg cgaatatacg gtttacattc cgggcattgt gaaagtaaca gcgcaatatt
    31261 ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca gggcatcccg
    31321 aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa agtcgtagcc
    31381 agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta ccagcggcac
    31441 agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt gcaaggcgtt
    31501 acgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt gcaggtcgag
    31561 ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg ggtcagtatc
    31621 gaggaagata ggtaagcgca tgttttctcc ataaaaaatg ccgataacaa gaccggcatt
    31681 ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac acgcagctct
    31741 ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc ttcaccacca
    31801 cccagctgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc cgatgcgccc
    31861 gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc tttcgcatcg
    31921 tcaatcaaaa acgcgcgttt gcccatacgc tgggccagct ccgccagacg gttggagttg
    31981 gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag ggcgcgtacc
    32041 gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc gacaattttc
    32101 gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catctaccga cagcgtggtt
    32161 tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac atcgtcaggc
    32221 gattcgacca gatacattcc cccttcaggg ttgctgtact gccccatcgt cccttccact
    32281 tccgggtgcc cggcgtgacc gatgagaata gactcttcgc cacgacggct ggcgcgggcg
    32341 acttccatat gcactttggt caccagcgga caggtggcgt cgaataccgt caaatcacgg
    32401 cttttcgctt cgttacgtac cgcctgagaa acaccatgtg cggagaagat caggatcgcg
    32461 ccgtccggca cttcgctgat ctgctcaata aagatagctc cacgctcgcg caggctatcg
    32521 accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc gtaaatggca
    32581 agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc acgtgggttg
    32641 gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca cttcaatatc
    32701 aaaatgaacg gtctgcccgg ccagcggatg gttgaaatcg acggtaatgg agtcgccgtt
    32761 aatttcgcgg atcacgccag gcatctcact gccatccatt gcggtaaaaa gcattattgc
    32821 gccaatttct ggctcgcctg catccataaa ctcacggcgg gagaagtact gaatcaggtc
    32881 cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaagtggttt tatcgcccac
    32941 tttcagcccc agcaggtgtt gctccagccc ttcggaaaga gaagcatcgc ccaggcggaa
    33001 cagcgccggt ttaccgttgt tgcgggtaga ctcggcggtg gtgccatcgt cgagttttag
    33061 cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc aggtttgctc
    33121 ttatattttg aggatttttg ccggatgcgg cctacgattt cgcagcaatc tgtaggcctt
    33181 atccggcagg gtttattatt gttttttcgc tttagaaggc aaaaaacctt ccagcacaat
    33241 cagtgccgca ccaacacaga tggcagtatc ggcgaggttg aaggtggcga agtgccagtc
    33301 gccgacgtag aagtcgatca tatcgacaac gaagccgtgc cacaggcggt cgaacaggtt
    33361 gcccagcgcg ccgccaataa tcagcgcgta ggcgatattg tttagcttct gcgtggcctt
    33421 cgagcgatac atcatcacaa ccagggtaac gctaatacca atcgcaatac cggcaaagaa
    33481 ccaacgctgc cagccgccgc tatcggcaag gaaactaaac gccgcgccat agttacgcgc
    33541 ataatgcaga ttaagcgacg ggaacagcgg gaccgtatcc cccagagcaa agttctggag
    33601 gatcaggtat ttgctgccca gatcgataat cagcacgact accaccagcc acagccagcg
    33661 tagccctgtt gaacagatcg attgactcat caggcaaact tacgtttttc accgtcaccg
    33721 gcgacgttgc tgacacagcg gccgcagatt tctgcgtgtt ccgccacctt gccgacatcc
    33781 tgggtgtagt gccagcagcg tgggcacttc tcaccttcgg ctttactcaa cgcgactttc
    33841 agccctttga gcacttcgct ctgctgagca tcagcaggtg cgtcgttata gtctgcaacg
    33901 gtagcgccgg aggtcaacag gacaaatcgt aattcatcgc ccaatgcggt cagtttcgcc
    33961 gccagttccg gttctgtata caaggttact gccgcttcca gcgagccacc cactttcttg
    34021 tcggcacgcg cctgctcaat gactttgttc acttcgccac gcactttcaa cagctcgtcc
    34081 cagaacgcat cgttcattgc ttcactgtct gccagaccaa acagaccttc gtaccactcg
    34141 ccggtgaaga cgtatttttc acgttcgccc ggcaggtagc cccacacttc atcagcggtg
    34201 aaggagagga ttggtgccat ccagcgaacc agcgcttctg cgatgtgata cagcgcagtc
    34261 tggcagctac gacgcgccac gctgtccgct ttggcggtgt actgacggtc tttgatgatg
    34321 tcgaggtaga aggaacccat ctcaacggag cagaagcgca tcagacgctg taccacttcg
    34381 tggaaatcgt atgcttcgta tgccttgagg atgtcttcct gtgccgcttt cgcacaacct
    34441 acggcccagc gatccagtac taccatctct tccggtttca ccatatcttt tgccggatca
    34501 aaaccgttca ggtttgccag caggaagcgc gcggtgttac ggatacgacg atagctgtcg
    34561 gcagcacgtt tcaggatctc gtcagaaacg gccatttcac cggtgtagtc ggttgatgcc
    34621 acccacagac gcagaatatc cgcgcccagt ttgttcatca catcctgcgg cgacacggtg
    34681 ttgccgatgg atttagacat cttgcggccc tgaccatcta cggtaaagcc gtgggtcagc
    34741 acctgacgat acggcgcttt gcccttcatc gcggtggaga tcatcagaga agacatgaac
    34801 cagccacggt gttggtcaga accttccaga tacatgtccg cagcgtgacc ggcaaattcc
    34861 gggcgcacgt caacaacaga agagtgagta gagccggagt caaaccatac atccagcgta
    34921 tccggcactt tcacatactg atcagcttca tcgccgagga tctctttcgc atcaagatcc
    34981 caccacgcct ggatgccatc aacttcaacg cgtttagcca cttcttccat cagttcgagg
    35041 gtacgcggat gcagctcttc cgtgtctttg tgcacgaaca gagacatcgg tacgccccag
    35101 gtacgctgac gggagataca ccagtcagga cggttagcga ccatcgactc gatacgcgcc
    35161 tggccccagt ccgggatcca ctgcacacct ttgatctctt tcagagactg cgcgcgcaga
    35221 cctttctgat ccatactgac gaaccactgc ggcgttgcgc ggaagatgat cggcgttttg
    35281 tgacgccagc agcacggata gctgtgctgc attttctcaa cgtgcagcag cgcgcctttt
    35341 tcctgcagca gcgcaacgac gatgtcgttc gctttgaaga cgttcacgcc atccagcgtc
    35401 ggataagtgc ccggcagata agtgccgtcc gggccaaccg ggttagcggt ttccaggccg
    35461 tatttctgac cgatcacata gtcgtccggg ccgtggccag gcgcggtgtg aacggcaccg
    35521 gtaccggcat ccagggtaac gtgatcgccg aggattgccg ggacgtcgaa gcccataaac
    35581 ggatgggcaa agcgcagcag ctcaagctcc gcacctttta ccgtgccgag aatggtgtaa
    35641 tcggtcacgc cgatacgctg cattacgctt tcaaccagat ctttcgccag aatcacggcc
    35701 tgaccgtcga tctgcaccag cgcatagtcg aaatcaggtg caatagagat tgcgcggttc
    35761 gcaggcagag tccacggcgt agtggtccag atcaccagcg agattgggcc gttaacgttg
    35821 cttacgccaa atttcgcttt cagcgcatcc tgatcgaccg cctggaaagc gacgtcaatg
    35881 gacggagaag ttttgtcgta atactcaact tccgcttctg ccagtgcaga acggcagtct
    35941 acgcaccagt gcaccggctt cgcgcctttg tgcaggtgac cattgccgat gattttgccc
    36001 agcgcgcgga tgatattggc ttcagttttg aagtccatgg tcaggtacgg gtgcgaccag
    36061 tcgcccagca cgcccagacg gataaagtct ttgcgttgac cgtcaacctg ggtcgccgcg
    36121 tattcgcggc acttggcgcg gaactcggcg gcggtgaatt tctcatccgg cttaccgtat
    36181 tcttgctcta ctttcagctc gatcggcaga ccgtggcagt cccagccagg cacatacggc
    36241 gagtcataac cggaaagccc tttggacttc acgataatgt ctttcagaat cttgttaacc
    36301 gagtgaccaa tatgaatgct gccattcgca taaggagggc catcatgcag aatgaaggtt
    36361 tttttgcctt ttttagccgc acgaatgatg ccgtacagat catcatcagt ccaacgcgcc
    36421 agcattcccg gttcgcgctt ggcgagatcg ccacgcatcg ggaaccctgt ttccggcaaa
    36481 ttcagggttg atttatagtc actcatcaga ttctcggttc cgtgtttcgg tttgattaca
    36541 taacaggctt aagccggttt tgttagccca aaaaattcgc gggcggttaa ttcatcacgc
    36601 gcaatctgcg ctttcagttc gtccagcgaa gcaaatcgct gctcattgcg tattttttta
    36661 cgcagcacta cttgtatatg gcgaccgtaa aggtccattg caacatctaa caaatgcact
    36721 tccagctgct ggcgaatacc ggcaaccgtt gggcgtgttc cgatgtttgc cacgccgggt
    36781 aacggctttt caccgaggcc cagcacttct accgcataaa cccctttcac cggggaaacc
    36841 tgacggcgta gcggtacatt cgccgtcggg aaacctatag tgcgccctaa ttcatcaccg
    36901 tggactacac gcccggagat ggcaaacggg tgccccagta aactttctgc cagagccaaa
    36961 ttgtcatccg caagggcctg acgcacggcg gtgctgctga tacgcacgcc accttcgcaa
    37021 aaagtttgcg tactggtgat atcgaagccg tattccatgc cagctttctg taataacaag
    37081 aaatcgcctt cccgaccagc gccaaagcgg aaatcatccc ccacggcgag gaattttacc
    37141 cgcaaatgct tcaccagaag atcgctgatg aaattttgcg cggttaacgc cgcgaaacgc
    37201 ctgtcgaaac gtacgcacag cacgtaatca acgccacact ctgcaaggta acgtagtttt
    37261 tcccgcagtc gggtcagccg tgccggggct ttatcggtag caaacagttc cagtggttga
    37321 ggttcaaaaa gcatcaccat caccggtaag ttgcgcttgc gcccttcttc ctgcaagccc
    37381 tgtaacagcg cgcgatgacc gcgatgcacg ccgtcgaaat taccaatagt cagcacacac
    37441 ccttcttgcg gggcctggct gagattatgt atgccgcgta tcagcttcat gtctggctca
    37501 aaacagtgaa aatcgtccga gtataccttg tacagcggtc aaggttaacc ggcgattgag
    37561 taccgaaatc agaaagaagt ggcttttcat tgtcatggtc atggcgcaaa tcacgggaag
    37621 aaactgactg cctgctgcaa tttttatcgc ggaaaagctg tattcacacc ccgcaagctg
    37681 gtagaatcct gcgccatcac tacgtaacga gtgccggcac attaacggcg cttatttgca
    37741 caaatccatt gacaaaagaa ggctaaaagg gcatattcct cggcctttga attgtccata
    37801 tagaacacat ttgggagttg gaccttggct aatatcaaat cagctaagaa gcgcgccatt
    37861 cagtctgaaa aggctcgtaa gcacaacgca agccgtcgct ctatgatgcg tactttcatc
    37921 aagaaagtat acgcagctat cgaagctggc gacaaagctg ctgcacagaa agcatttaac
    37981 gaaatgcaac cgatcgtgga ccgtcaggct gctaaaggtc tgatccacaa aaacaaagct
    38041 gcacgtcata aggctaacct gactgcacag atcaacaaac tggcttaatt gccaatttgc
    38101 tgaagctttg tgaaaaagcc cgcgcaagcg ggttttttta tgcctgctgc ttttgtgatg
    38161 cgtgagagaa cacactgccg gttgccagat agtcaaaaga gacatgccgt tgcactatct
    38221 gaactcttcg tggcttaaga atcgtagaac catcggctga tacattctga acaagaatat
    38281 ctactcaatc tccagactcc cctacaaaga atgtggatat tgtgatacac attgaggtat
    38341 taaacttaag accactctat ttttgaatta aattcctata tactttaacc attcataaat
    38401 atctcctcat tagaccttgt aaattattaa cagcaatcaa cacaggttaa atacctattt
    38461 atattgtact tcttaaaatt aatgagcgat ttttgatagt agtcacattc actcataaat
    38521 taaattaata aaaattcatc aacttcaagg actgataatg acagatggta tctcaacttc
    38581 gccacattgt ctttataaat caaatatagt agatgatgta ataataaata aaacaagaca
    38641 aaatgagtta gtaaaagtgt tttgcgagta taaaacagag ttcttaattt tgtttgatga
    38701 ctttttcaga agccaggatt tgcctaaacc atctccagtc ctccatcatt tttttcaata
    38761 tactcatctg agagatgcac atttttatag atgtaaactc atagaacata cagttcaatt
    38821 ttcttttttt aaacacaaag gcatcacatt acttagattg gatgtttttg atgacagaac
    38881 aagcgaatgt ctttctgaag agattaagat atatcaagaa tgccatgaaa aatttattaa
    38941 atttttaaag gcaaacttca atcaggaaat atacccagag ctatacactc ctgaaatatt
    39001 ttatgaggcc tgcagaaatc tccagtcatt ttatgatcat caggagacgt cacaaaacgc
    39061 taagtacagt gcaatagtca agaaaaaaag ttattttaat aaggaaatca gaaatctaat
    39121 aaaaaagaat atttatcctg agctatacaa tgagcaatgc aataaaatcc ccgcctcatc
    39181 gacagatgat aaccagaaaa taacatggca gaattttaaa actagcaatg cggcatattc
    39241 acaactatgt gaaaaattat ctttattaaa aagctcgccc agtcggttaa tagagaaatc
    39301 agcttactgc tcaaatgaaa acatgatcac cgataaattt gatgttgtct tttcttactg
    39361 cggagataat gtaaaggaat ttatactttt attaccctat aataaatcgc tggagatgca
    39421 tgaacttaac ga
//
LOCUS       DABSYT020000033        39144 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.033, whole genome shotgun sequence.
ACCESSION   DABSYT020000033 DABSYT020000000
VERSION     DABSYT020000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.033"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..115
                     /gene="ghoS"
                     /locus_tag="ID725_RS10635"
                     /old_locus_tag="pgaptmp_002127"
     CDS             <1..115
                     /gene="ghoS"
                     /locus_tag="ID725_RS10635"
                     /old_locus_tag="pgaptmp_002127"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGV7850730.1"
                     /translation="AASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            143..316
                     /gene="ghoT"
                     /locus_tag="ID725_RS10640"
                     /old_locus_tag="pgaptmp_002128"
     CDS             143..316
                     /gene="ghoT"
                     /locus_tag="ID725_RS10640"
                     /old_locus_tag="pgaptmp_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGV7850731.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(435..1952)
                     /gene="lysS"
                     /locus_tag="ID725_RS10645"
                     /old_locus_tag="pgaptmp_002129"
     CDS             complement(435..1952)
                     /gene="lysS"
                     /locus_tag="ID725_RS10645"
                     /old_locus_tag="pgaptmp_002129"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGV7850732.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(2189..3646)
                     /gene="dtpC"
                     /locus_tag="ID725_RS10650"
                     /old_locus_tag="pgaptmp_002130"
     CDS             complement(2189..3646)
                     /gene="dtpC"
                     /locus_tag="ID725_RS10650"
                     /old_locus_tag="pgaptmp_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGV7850733.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(3705..5852)
                     /gene="cadA"
                     /locus_tag="ID725_RS10655"
                     /old_locus_tag="pgaptmp_002131"
     CDS             complement(3705..5852)
                     /gene="cadA"
                     /locus_tag="ID725_RS10655"
                     /old_locus_tag="pgaptmp_002131"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGV7850734.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(5932..7266)
                     /gene="cadB"
                     /locus_tag="ID725_RS10660"
                     /old_locus_tag="pgaptmp_002132"
     CDS             complement(5932..7266)
                     /gene="cadB"
                     /locus_tag="ID725_RS10660"
                     /old_locus_tag="pgaptmp_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGV7850735.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7632..9170)
                     /gene="cadC"
                     /locus_tag="ID725_RS10665"
                     /old_locus_tag="pgaptmp_002133"
     CDS             complement(7632..9170)
                     /gene="cadC"
                     /locus_tag="ID725_RS10665"
                     /old_locus_tag="pgaptmp_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGV7850736.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(9430..9589)
                     /locus_tag="ID725_RS10670"
                     /old_locus_tag="pgaptmp_002134"
                     /pseudo
     CDS             complement(9430..9589)
                     /locus_tag="ID725_RS10670"
                     /old_locus_tag="pgaptmp_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(9787..9862)
                     /locus_tag="ID725_RS10675"
                     /old_locus_tag="pgaptmp_002135"
     tRNA            complement(9787..9862)
                     /locus_tag="ID725_RS10675"
                     /old_locus_tag="pgaptmp_002135"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9827..9829),aa:Phe,seq:gaa)
     gene            complement(9969..10544)
                     /gene="yjdC"
                     /locus_tag="ID725_RS10680"
                     /old_locus_tag="pgaptmp_002136"
     CDS             complement(9969..10544)
                     /gene="yjdC"
                     /locus_tag="ID725_RS10680"
                     /old_locus_tag="pgaptmp_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGV7850737.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(10581..12278)
                     /gene="dsbD"
                     /locus_tag="ID725_RS10685"
                     /old_locus_tag="pgaptmp_002137"
     CDS             complement(10581..12278)
                     /gene="dsbD"
                     /locus_tag="ID725_RS10685"
                     /old_locus_tag="pgaptmp_002137"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGV7850738.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAQLRDRQP"
     gene            complement(12254..12592)
                     /gene="cutA"
                     /locus_tag="ID725_RS10690"
                     /old_locus_tag="pgaptmp_002138"
     CDS             complement(12254..12592)
                     /gene="cutA"
                     /locus_tag="ID725_RS10690"
                     /old_locus_tag="pgaptmp_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGV7850739.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(12708..14009)
                     /gene="dcuA"
                     /locus_tag="ID725_RS10695"
                     /old_locus_tag="pgaptmp_002139"
     CDS             complement(12708..14009)
                     /gene="dcuA"
                     /locus_tag="ID725_RS10695"
                     /old_locus_tag="pgaptmp_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGV7850740.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(14127..15563)
                     /gene="aspA"
                     /locus_tag="ID725_RS10700"
                     /old_locus_tag="pgaptmp_002140"
     CDS             complement(14127..15563)
                     /gene="aspA"
                     /locus_tag="ID725_RS10700"
                     /old_locus_tag="pgaptmp_002140"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGV7850741.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            15900..16376
                     /gene="fxsA"
                     /locus_tag="ID725_RS10705"
                     /old_locus_tag="pgaptmp_002141"
     CDS             15900..16376
                     /gene="fxsA"
                     /locus_tag="ID725_RS10705"
                     /old_locus_tag="pgaptmp_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGV7850742.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(16395..17648)
                     /gene="yjeH"
                     /locus_tag="ID725_RS10710"
                     /old_locus_tag="pgaptmp_002142"
                     /pseudo
     CDS             complement(16395..17648)
                     /gene="yjeH"
                     /locus_tag="ID725_RS10710"
                     /old_locus_tag="pgaptmp_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
     gene            17924..18217
                     /gene="groS"
                     /locus_tag="ID725_RS10715"
                     /old_locus_tag="pgaptmp_002143"
     CDS             17924..18217
                     /gene="groS"
                     /locus_tag="ID725_RS10715"
                     /old_locus_tag="pgaptmp_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGV7850743.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            18261..19907
                     /gene="groL"
                     /locus_tag="ID725_RS10720"
                     /old_locus_tag="pgaptmp_002144"
     CDS             18261..19907
                     /gene="groL"
                     /locus_tag="ID725_RS10720"
                     /old_locus_tag="pgaptmp_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGV7850744.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            20045..20398
                     /gene="yjeI"
                     /locus_tag="ID725_RS10725"
                     /old_locus_tag="pgaptmp_002145"
     CDS             20045..20398
                     /gene="yjeI"
                     /locus_tag="ID725_RS10725"
                     /old_locus_tag="pgaptmp_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGV7850745.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(20591..21460)
                     /gene="yjeJ"
                     /locus_tag="ID725_RS10730"
                     /old_locus_tag="pgaptmp_002146"
     CDS             complement(20591..21460)
                     /gene="yjeJ"
                     /locus_tag="ID725_RS10730"
                     /old_locus_tag="pgaptmp_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGV7850746.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(21695..22723)
                     /gene="epmB"
                     /locus_tag="ID725_RS10735"
                     /old_locus_tag="pgaptmp_002147"
     CDS             complement(21695..22723)
                     /gene="epmB"
                     /locus_tag="ID725_RS10735"
                     /old_locus_tag="pgaptmp_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGV7850747.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            22765..23331
                     /gene="efp"
                     /locus_tag="ID725_RS10740"
                     /old_locus_tag="pgaptmp_002148"
     CDS             22765..23331
                     /gene="efp"
                     /locus_tag="ID725_RS10740"
                     /old_locus_tag="pgaptmp_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGV7850748.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            23383..23508
                     /gene="ecnA"
                     /locus_tag="ID725_RS10745"
                     /old_locus_tag="pgaptmp_002149"
     CDS             23383..23508
                     /gene="ecnA"
                     /locus_tag="ID725_RS10745"
                     /old_locus_tag="pgaptmp_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGV7850749.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            23619..23765
                     /gene="ecnB"
                     /locus_tag="ID725_RS10750"
                     /old_locus_tag="pgaptmp_002150"
     CDS             23619..23765
                     /gene="ecnB"
                     /locus_tag="ID725_RS10750"
                     /old_locus_tag="pgaptmp_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGV7850750.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            23941..24258
                     /gene="sugE"
                     /locus_tag="ID725_RS10755"
                     /old_locus_tag="pgaptmp_002151"
     CDS             23941..24258
                     /gene="sugE"
                     /locus_tag="ID725_RS10755"
                     /old_locus_tag="pgaptmp_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGV7850751.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(24255..24788)
                     /gene="blc"
                     /locus_tag="ID725_RS10760"
                     /old_locus_tag="pgaptmp_002152"
     CDS             complement(24255..24788)
                     /gene="blc"
                     /locus_tag="ID725_RS10760"
                     /old_locus_tag="pgaptmp_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGV7850752.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(24876..26009)
                     /gene="ampC"
                     /locus_tag="ID725_RS10765"
                     /old_locus_tag="pgaptmp_002153"
     CDS             complement(24876..26009)
                     /gene="ampC"
                     /locus_tag="ID725_RS10765"
                     /old_locus_tag="pgaptmp_002153"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGV7850753.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(26072..26431)
                     /gene="frdD"
                     /locus_tag="ID725_RS10770"
                     /old_locus_tag="pgaptmp_002154"
     CDS             complement(26072..26431)
                     /gene="frdD"
                     /locus_tag="ID725_RS10770"
                     /old_locus_tag="pgaptmp_002154"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGV7850754.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(26442..26837)
                     /gene="frdC"
                     /locus_tag="ID725_RS10775"
                     /old_locus_tag="pgaptmp_002155"
     CDS             complement(26442..26837)
                     /gene="frdC"
                     /locus_tag="ID725_RS10775"
                     /old_locus_tag="pgaptmp_002155"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGV7850755.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(26848..27582)
                     /gene="frdB"
                     /locus_tag="ID725_RS10780"
                     /old_locus_tag="pgaptmp_002156"
     CDS             complement(26848..27582)
                     /gene="frdB"
                     /locus_tag="ID725_RS10780"
                     /old_locus_tag="pgaptmp_002156"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGV7850756.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(27575..29383)
                     /gene="frdA"
                     /locus_tag="ID725_RS10785"
                     /old_locus_tag="pgaptmp_002157"
     CDS             complement(27575..29383)
                     /gene="frdA"
                     /locus_tag="ID725_RS10785"
                     /old_locus_tag="pgaptmp_002157"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGV7850757.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29708..30685
                     /gene="epmA"
                     /locus_tag="ID725_RS10790"
                     /old_locus_tag="pgaptmp_002158"
     CDS             29708..30685
                     /gene="epmA"
                     /locus_tag="ID725_RS10790"
                     /old_locus_tag="pgaptmp_002158"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGV7850758.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            30904..32406
                     /gene="yjeM"
                     /locus_tag="ID725_RS10795"
                     /old_locus_tag="pgaptmp_002159"
     CDS             30904..32406
                     /gene="yjeM"
                     /locus_tag="ID725_RS10795"
                     /old_locus_tag="pgaptmp_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGV7850759.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(32557..35880)
                     /gene="mscM"
                     /locus_tag="ID725_RS10800"
                     /old_locus_tag="pgaptmp_002160"
     CDS             complement(32557..35880)
                     /gene="mscM"
                     /locus_tag="ID725_RS10800"
                     /old_locus_tag="pgaptmp_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGV7850760.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(35902..36870)
                     /gene="asd"
                     /locus_tag="ID725_RS10805"
                     /old_locus_tag="pgaptmp_002161"
     CDS             complement(35902..36870)
                     /gene="asd"
                     /locus_tag="ID725_RS10805"
                     /old_locus_tag="pgaptmp_002161"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGV7850761.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(36967..38019)
                     /gene="rsgA"
                     /locus_tag="ID725_RS10810"
                     /old_locus_tag="pgaptmp_002162"
     CDS             complement(36967..38019)
                     /gene="rsgA"
                     /locus_tag="ID725_RS10810"
                     /old_locus_tag="pgaptmp_002162"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGV7850762.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            38114..38659
                     /gene="orn"
                     /locus_tag="ID725_RS10815"
                     /old_locus_tag="pgaptmp_002163"
     CDS             38114..38659
                     /gene="orn"
                     /locus_tag="ID725_RS10815"
                     /old_locus_tag="pgaptmp_002163"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGV7850763.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            38870..38945
                     /locus_tag="ID725_RS10820"
                     /old_locus_tag="pgaptmp_002164"
     tRNA            38870..38945
                     /locus_tag="ID725_RS10820"
                     /old_locus_tag="pgaptmp_002164"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38903..38905,aa:Gly,seq:gcc)
     gene            38982..39057
                     /locus_tag="ID725_RS10825"
                     /old_locus_tag="pgaptmp_002165"
     tRNA            38982..39057
                     /locus_tag="ID725_RS10825"
                     /old_locus_tag="pgaptmp_002165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39015..39017,aa:Gly,seq:gcc)
BASE COUNT         9821 a         9717 c         9901 g         9705 t
ORIGIN      
        1 agccgcaagt gaaatcgaag atttaatcag gcttaaatgc ttagacttac cggatattga
       61 ctttgacctc aacattatga cagttgatga ctatttccgt cagttttaca agtagccagt
      121 aaggataaga agaacataaa aaatggcact attctctaaa atattaattt tttatgtgat
      181 tggtgtgaac atatcctttg tcattatctg gtttatctca catgagaaaa cacatattcg
      241 tttacttagt gcattcctgg tcggaataac ctggccaatg agtctgcctg tggcattact
      301 tttttctctc ttttaggagc tgtagttgag tggacccgct ggttatcgtg aaacgaagcg
      361 tctctcctca aaactggatt tgcggaacga tgaacgaagc agtcgcctga ctgcttcgtt
      421 cattaaagtg aaatttattt ctgtgggcgc atcgccggga agagaataac gtcgcgaata
      481 gtatggctgt tagtaaacag cataatcatt cggtcgatac caatacccag accagcggtt
      541 ggcggcagac catattccag cgcagtcacg taatcttcgt catagaacat ggcttcgtcg
      601 tcacctgcgg ctttagcgtt aacctgttcc tggaaacgtt cagcctgatc ttctgcgtcg
      661 tttaattcgc taaaaccatt accgatttca cgaccaccga tgaagaattc aaaacggtcg
      721 gtgatttccg ggttaacatc attacggcgt gccagcggtg acacttctgc cggatattcc
      781 gtaataaagg ttggctgaat cagatgtgct tctgccactt catcaaagat ctccgtgaca
      841 atacgtccca gcccccagct tttctctacc gtaataccga tagattcagc taatgcttta
      901 gcagcatcaa aattatccag gtcggccata tcggtttctg gacgatattt tttgattgct
      961 tcgcgcatgg tgagtttttc aaacggtttg ccgaaatcaa acacatgctc gccataagtg
     1021 actttagtgg tacccagaac ctcttgtgcc agggtgcgga acagggactc tgtcagttca
     1081 atcaaatcgt ggtaatccgc atacgccatg tagagttcca tcattgtgaa ctcaggatta
     1141 tggcgaacag aaataccttc attacggaag ttacggttga tttcgaatac ccgttcaaaa
     1201 ccgcctacaa ccagacgttt cagatacagc tccggcgcga tacgcagata catatctaaa
     1261 tccagagcat tatgatgggt aataaacggg cgagcagatg ccccacctgg aattacctgc
     1321 atcatcgggg tttctacttc cataaagccg cgcgcgacca tgaactggcg gatagcggcc
     1381 agaatttttg aacggacaac aaacgtttga cgggatttat cgttagcgat gaggtccaga
     1441 taacgttgac gataacggac ttcctgatcc tgcagaccat ggaatttatc tggtaaagga
     1501 cgtagtgctt tagtcagcag gcgcaactca gtacagtgaa tggaaagctc acccgtttgc
     1561 gtcttaaaca gcgtaccgcg ggcaccgata atgtcaccca gatcccattt tttaaactga
     1621 tcgttataaa caccttctgg caggctatct cgtgcgacgt acagttgaat acggccaccg
     1681 acatcctgca acgttacaaa ggaggctttc cccatgatac gacgggtcat cattcggcca
     1741 gcaaccgaga cttcaatgtt taaggattcc agttcctggt tatccttcgc atcaaactct
     1801 tcgtgcaact ggtcagaggt atggtcgcgg cgaaaatcat tgggaaacgc cacaccttgc
     1861 tgacgtagtg ccgccagttt ttcgcggcga tttctcagtt catcgttaaa atcaatagcc
     1921 tcattggctc cccgtgtttc ttgttcagac attttggttc ctctaaatcc agctttcaat
     1981 ttagcgtaga taaagagaca gatcggtaca ctgtattatc tgcctcgact attaataact
     2041 cagtttatct tatcgaaact aacgcttacc tcaagttgat atcactaata aataaccatc
     2101 aaaatggttt aaatgactta ttaatctcga tttactagtg tgataaactg aaagttaatt
     2161 tattcataac caattgtttt tacgacagtt aatcgttgct ctcctgtacc atattcgtcg
     2221 gcgtgctaaa caggaaacgg gtagcaaagg ccaataccac gatgatcgcg acacaagcca
     2281 acgtccactc tcccatctga gaaaagaatc gctggtaagc tgcaattgcc ataccgctaa
     2341 tttgcgactc tgtcgtctgc tgtgccacta cgcctgccag ccagttggcg accgcgcctg
     2401 ttgccagcat ataaatcccg gttaatacgc cagacatttt cagacgcgtg atttgcgcaa
     2461 tcgccaccgg gtcaataaag agttcggcaa agcccattag cgccagcccg gataccatca
     2521 cgcccattga cgcttgaccg tcagctgctg catgtcgggc atcaaatgcc agcaacataa
     2581 agccacaagc catcagcagt aagccaaagg caaacttcag ccagacgcgc aatgttgagt
     2641 tgccgtggct ttctggcgac gccagccagg ccagaacaac cccagcgagc atcaccgcaa
     2701 ttgcattcac cgactggaat agtgcagtag gtacttcaat attgaatgcc tgacgattca
     2761 caaagcgatc gataaacaag ctgatggtac tgccgccctg ttgtgccagt acccagaaca
     2821 atgtcccgac aaacatcaac aatacaattt gccaaagagc acggcggtgt tcggggaatt
     2881 tgatcatcat gcgggcaatg atttgtgcgg caatgaggca aacgatcgcc agcaaatatc
     2941 cggaccagtc gttctccagc agcagggtaa aaaatactgg ggctaaacag agcatcacca
     3001 ctaaccagct ccataccggt aaggcaaatt tgacgctagt gagcgctttt ttatccatac
     3061 tacgcgtgga ctggaaatga cgatgcccgc ttaagaaaat caacaaaccg ataaacatgc
     3121 cgccacccgc aagggcaaag ccaacatgcc atccatacca ctgagcagcc aggccgcagg
     3181 cgattggggc ggcgatagaa ccgatattgc ccgcagcata cagcagcgaa aaaccgccat
     3241 cacgtcgatg atcgttctcg tcgtagagct cgccaagcaa acagctgatg tttgatttga
     3301 ataaaccgta gccacaaata atgattgcca gcgccagata caggctaaag gttgaatttg
     3361 tatcaatacc cagcaccaca tggccaaggg tcattaacag cgcgccggca atcactgcag
     3421 tgcggttgcc gagcaggcgg tcggcaagcc agccgccgag aataggggta acgtaaacca
     3481 gagaagcata tgcgctgaac aggctgatgg catggttatc atcaaaacca agctgatggg
     3541 tgagatagag aatgagtaag gcacgcatgc cgtaaaaact gaagtactcc cagatttgga
     3601 tcgccacgat atagtatatc gcgcgcggct gtgagggtgt tttcatgtgt tctccttatg
     3661 agcaaaaaag ggaagtggca agccacttcc cttgtacgag ctaattattt tttgctttct
     3721 tctttcaata ccttaacggt atagcggcca tcagcctgac ggtatgcacc gtgaatatcg
     3781 gtttcaaagc ccggatagtg agcgccgatt tcacacagca tctgcaggaa ctccagaacc
     3841 ggacggcttt cttcggtgat catttcaccc ggcattacca gaggaactcc cggcggatac
     3901 ggaaggatca tattggcgtt aatacgaccg accatttcgt cgaggtaaac ttcttcggtc
     3961 ataccgtgca gctctttctg gaatgcagca tacggagtca ttaccatcgt cggcagcact
     4021 tcaaatgcgc gatacatcag atccggcaga ttgtggtgaa caatcagttt gtggatattc
     4081 tgagccagtt cctgaatacg catgttttca tagaattcag gatcttcacg atacagagac
     4141 ggcagcatgt ttttcacacg caggttcagg tcgaacgcac gtttgaagtc agtcagagca
     4201 cgcagcaggc tcagtgcttt ggtcttatcg ataccgatgc tgaacaggaa cagcaggtta
     4261 tacggaccgg ttttctcaac aacgatgcca tgttcgtcga ggtatttcgc cacgatgctg
     4321 gccggaatac caaagtcgct catggtgccg tctttttcca tccccggagt cagcagggtg
     4381 actttgatcg ggtcaagata catgtgctcg ttatcgatgt ttttgaagcc gtgccaggtg
     4441 ctgtcagaac gcagcggcca gcattcagtc gtatcgatat gatccggctg ccatacatca
     4501 aagaaccagc catcagattc cgttctcaga cgtttgatct ctttacggaa tttgatcgca
     4561 cgttcaatgg aaccgttgat cagacgctta cctgcattgc ctttcatcat cgccgcagcg
     4621 gtttcagtgg acgccacgat accgtagtgc ggagaagtgg tggtgtgcat catgtaggct
     4681 tcgttaaagg tttcttcgtt tacgtcacct ttaacgtgga tcatggaagc ctgagagaac
     4741 gccgccagca gtttgtgagt ggactgggtt tcgtaaatca ctttcccttc tacacggcca
     4801 ccgctcatac cgcatttacc ttcgtaaatc ggtgagaagt tggtgtaagg cacccacgcg
     4861 gagtcaaagt ggatggattt cacatccagt gttttcttga tgaagtcggt gttgtacagc
     4921 agaccatcat aggtagagtt ggtaattaca gcatgtaccg gccaggttgc gtttggtgtt
     4981 tctttcacgc gcttagcaat ggtagcgtgc tggaattcac tctgtgggat accaccaaga
     5041 ataccgtaag cgttacgggt cgggcggaaa tagattggcg taacatcgct catcatcatc
     5101 aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa tggtgctgcc tgccggagca
     5161 gagtacatac caacaatttt gttcgcagtg gaagtaccgt tggtcaccat gtagctgcgg
     5221 tctgcgttaa agacgcgagc gatatactgt tctgcttctt tgtgtggacc actgtgatcc
     5281 agcagagaac ccagttcaga tactgaaatg gaaatatcag atttcatggt attcggacca
     5341 aagaaatcat agaacaggct acctaccggg cttttctgga atgcagtacc acccatgtga
     5401 ccaggagtac agaaagtata tttaccttca cgaacatatt taaacagtgc tttagtcagc
     5461 ggaggcagaa tagtgttgat atattcgtca gtggtctgct tgatcttgtt agcaatatct
     5521 tcagcagcac ccagcgcata ttcaaagaag ctaatctgta aacgcaggtc attcaggctt
     5581 acatcgagag tggaatacgt attagcgaac gcgtacaacg gcaggttctc gttcattttg
     5641 ctaatttctt cgcacagctc gagattatat ttatcccagt caaaaataac gccgcacaga
     5701 cgcgcattgt tttcgatcag ttttaataag tcgtcacggt cgttcgggta aacaatctgg
     5761 aagttcagac gttcaagcgc gcgatgaagt tcacggatgg gttcttcttt aaaataaacc
     5821 cccatgtgat tcaatattgc aataacgttc atagtcatat ctccaggtaa aaaaggcccc
     5881 tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa aatactttta attaatgtgc
     5941 gttagacgcg gtgtggttat ccattgagtg gctctggcgc tcgtgcattt tgcgagcgta
     6001 gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc agctcgaagg agcttgcgcc
     6061 catcagcgcg atgaagcaga acacgcaacc cagtacagag cagatcaggc tgacaaagtt
     6121 gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa gagtagaagt acggcagcat
     6181 agtcagcagt actgcgatac cggtcagttc accgaacagg tcagatgctt taccaccggc
     6241 agagttcatc agagtgatga ggatcatcag ggcagtcatt ttcactgcag ccagcagcag
     6301 accttttttc ggaataccgt tgctgtcgac ttcaccataa actttcggga agttaccgtc
     6361 gttagcggca cgtacacctg cctggcctac caacatcatc caggagccca gagaagtcag
     6421 gcacgcaaag gcggtgaatg cagaaaccag cggcgcagcc cagttaccga ggatagttga
     6481 agcactgatt gcaaacggag caccggaagc cgccattaca gaagacggat acataccgga
     6541 aagcacctga gtcgcagcga tgtaaacaat acctgctaaa ccagtaccca gcatggttgc
     6601 cagcggaacg gtacgtttcg ggtttttaac cataccagta cttacagctg cggattcaac
     6661 acccacgaag gcccacaggc agagcagaat acttttaatg atcgcatgac catcagtggt
     6721 atccgcagta ttccagttag ctgcataagt tgccgcatca aaccaatgcc agccaacaat
     6781 agcagtcatc accacaggaa taagaaccag caccagacca atagtggtta aacggcttac
     6841 ccaggtaccg ccgagcatat ttacaaaggt aaatacccag acgatagcaa tacaggcgat
     6901 acccgccgga acaggatcat ttaatactgg gaagaaggtg gaaagataag atacagcggt
     6961 aataccaatc gccaggttac caatccagtt agcatggtaa taaagaacac ctgtctgaaa
     7021 accaaatgca ggagaaattt ctccggcata agcaattggg ccaccttgtt gcgggttttt
     7081 tgttgccagt cgggcatata catacgccag cgacattgca ccaataatag agataatcca
     7141 accccagata gcaataccac cgatacttgc taggttcgca ggtaataatg caataccgct
     7201 ccccatcata ttaccggcaa caacaccggt acaggcaaat agcccgatct tcttggcaga
     7261 actcatgctc ttctcctaat ttcatttttg aatttggagt ccgggtcatg atgtataact
     7321 atttcctgac cagaccaaac tggcgctaag attactcacg aaaaaaggat taatcctaaa
     7381 gattaggtga aataacacaa aagtttctgt aagtgagaac ttgaggtttt ttattaacac
     7441 atcaggatcg caagttgata tcatgaaaag ataaacattc aatgtttaca atagattgcg
     7501 tgacgtcttc tggttaaatt tatgtaataa aaattatgcg gcaaataaat tgccgcaaca
     7561 tattatacca acaggaacat acaaaaactc aacaacaaat attcccgagc atgaatcaac
     7621 ccgggggtta cttattctga agcgagaaat ttgtcgagat aaggtacaac ataaggaaca
     7681 gaagtctgga atataccatt ttcaatccag taaagggtgt ttgcccctgg gcgtaaatta
     7741 aaggcggtga gatatgcatc agccgcttcc cggttcatcc ccttcatttc ataaaccttg
     7801 ccaagcaata cataatttag ccaggacatt tcaagatcaa tgccagtatt tatcgcctgg
     7861 taagactcat ctgttttacc ttttaccaga gcactgaccg cttttatttg atatataatg
     7921 gacaggttat tcaattccgg tagtgtaaca atgttatcta tttctgtgtt cagtgctgct
     7981 aattgttttt catctaaagg atgttgagaa tggcgcacga tatcaactaa tgctttttct
     8041 gctctcgcgt aggtaaattc tggggatgat tgaacaatct cacctaataa ttcactggca
     8101 cggttcaatg atttatcatc gccatgcagt aaataatcat gtgcctgata aaaattagtt
     8161 aataacgcac cacgatgagg caaaatttgc tggagcgtct cctgcattcg ttgtggccac
     8221 ggttggttta acgcttttga taaactctcc agtaaatcat tttgaatcgc cagctgatta
     8281 ccgttagtga tgacataacg tttatccagc atggttgaac catctgcatt gtctaccaat
     8341 tttatcgaca taaagcattg ttgagcacgg tattggcgct gattaacaaa cgcaatagat
     8401 aatgttttac cggaactgct cggttcatca atgttgtagt tgattttgtc atgcaccata
     8461 aaggtggaga aggtgttaag tgatgtcgcc accaaatcac ccacgcctat cgcgtaagag
     8521 agctgatacg gggaactcca gctgttacaa cttttattta ccatattaat gtcaatatcg
     8581 cgtggattga gcaaaatacg cgatttgctc atcggaagac gtgtatcaag acttgaaaac
     8641 gctaccagtg ctacacagat acctaaggac aacaggaaaa aaaaccatac ccaaaaggta
     8701 gtgaatcgtt tgcttttaac tggggattgt tcaggtggcg ttgcggtgtt ttgaatgtta
     8761 agactgtggg agggagaatc tgtggcagga accgcctctg gtataggggg aggcgaagat
     8821 agcattattt cctctccctc ttcttcgctg taccagataa ccggcaccat taatttatag
     8881 ccgcgctttg gtacagtagc gatatagaca ggactatctt catcattatc ttttaatgac
     8941 ttacgtagtt ctgagatact ctgcgtcaca acgtgattgg tgacaatact tctcttccag
     9001 acattatcga taagttcatc cctgctaagt acttcgccac tgtgttgagc aaagaaaacc
     9061 agaaggtcga ttaatctcgg ctcaagggta agttgacgcc cattgcggct aatttggttt
     9121 atggacggag taacaagcca ttcgccaacg cgaactacag gttgttgcat aatagaaact
     9181 cattcgaaaa gggaatgatg caatgataat tgccacaacc tatttttacc atctatagat
     9241 gggtttattt acatattatt ggtgaatgca agacgttatt tttaccagcc ataaacttct
     9301 tgattacatg gtattacgaa aggattttac tgagaaccag aagtaatatt ccttaccatc
     9361 aaaattcatc atctttgcca aagaaaaatg ttcagaaaat aatccatgga aaattgtccg
     9421 gagcgcttac tattttaatg gattgttagt ctttgcatga gcaagcggac tgatacattt
     9481 ctctttgttc tcattcagaa aatctcatca gtcggcgttc agaccgatgt tgcgctttat
     9541 ggatgcgtta cgctcctgat gacgtcattt gacgttcaac agcatcacgg ggccgcacga
     9601 catttcacgt tagttagtgc tatagctcag gaacaaattt tcccgaattg ggatatgccc
     9661 gcaaattgct ggtgatgtgg ggagaatcgg gttgagttcg gtagaattga tttggaggca
     9721 gaacacttaa atcgtggcgt cctgaaacga aaaacggacc tccgtggagg tccgtttata
     9781 tgaatttggt gcccggactc ggaattgaac caaggacacg gggattttca atcccctgct
     9841 ctaccgactg agctatccgg gcaacggggc gcattaaacc gtaatctgca catctcgtca
     9901 acctaatttc agtaaaagcg attcaactgc ttaagattgc ggcaaatcgc ttcttttctg
     9961 tgtttctgtc aggtcagtgc accaccctgg cggcagcggg cgaagcgcag gatatcttcc
    10021 gccagccgat gtgcggtgtc gacatccgcc tggctacgat tcaccagcat tcggcttaaa
    10081 cagccttcca gcaccagttc catctgcttt gctaccatcg ccggatcgtc aacttccagc
    10141 gtggttaaca gttcgtgggt gaaatcgtag gccgcgcttt tttgctgatc ggccagttga
    10201 tgaatagggt ggccaggatc gggataaaac gtacaggcag cgataaacag acagcccgga
    10261 tagcggttgt ttttaacgca ctccgataac gcctgataac gtgccagcag cttttgttcg
    10321 gcggtttgcg tttcgtccag catcagctga cgacgccaga catctatctg ttggctaaga
    10381 taacgcagcg catcgtagag gattgcctct ttgtctggcc agaagcggcg tagctcgtcc
    10441 agtggataat ccacacgttc agcaaccatc tccagcgtgg tgttggcaat cccttgtaat
    10501 tctaataatt tcagggcttc tcccagtaca tcttcacgtt gcacgctatt ttcctccgtc
    10561 tttcccactg caagtgtcgt tcacggttgg cgatcgcgca attgtgcgct gaaggtttca
    10621 gcatccataa agcccgtgac gcgtgcttgt ggatgctcct ggccttgtcc gtcaaaaaag
    10681 agaattgtcg gtaggccaag gacattaaga tgctttaaca gcgccacatc ttgtgcgtcg
    10741 ttggcagtga cgttggcctg aagtaatacc gtgtctgcta acgctttttg cacctgcggg
    10801 tcgctgaagg tgtatttctc aaactcttta caggcgacgc accagtcggc ataaagatct
    10861 aacatcaccg gtttgccttt ggcttcaacg agcgcctgat ttaactcatc taccgttttg
    10921 atttgtgtaa agttgagatg cgtctgagtt tgcgcggtat gcgtcgcacc aaatgcccaa
    10981 tcctgaagtg ggcgcacgct aaccaatgcc gctgcaagca ggattatttg caccacgcgc
    11041 atccagccgc gtttggcctg taggctggtg ataaaggccc agccaaagaa tgcgacaccc
    11101 agcgccgacc acaagcgtaa tccccataca tcaccaatca ctcgctccag caggaagacc
    11161 ggcagtgcga ggatcacaaa accaaacgcg gttttgactt gttccatcca cgggccgctt
    11221 ttcggcagca agcggttacc aaagacggta attagcatca gcggcaggcc catgcccaac
    11281 gcatagagat aaagcgtgcc gccgcccagc cacatgttcc cgctttgggc gatatacagc
    11341 agaatcgcgc taagcggtgc ggtggtgcat ggtgaacaga tcagtccggc aatcgtcccc
    11401 ataataaaca ctccgccggg tgagccgccc tgttggcgat tgctcatcag cgtgaggcgc
    11461 gtttgcagcg aggaggggag ttgtagagta agcaagccaa acattgacat cgccagcaag
    11521 gtaaagacga tggtgaggcc aatgagcacg tatgggtgct gtagcgccgc ctggaactgt
    11581 aaccctgcgg cggcaactac cagacccagc gccgtgtaag tcagtgccat cccctgcaca
    11641 taaataaagg tcagcaacaa tgctctggca gtggagagcc gctgtttacc acccagcacg
    11701 atgccagaaa tcagtgggta cattggcagc acgcatggcg taaaggcgat accaataccg
    11761 atcaacaacg cccagagcgc ggaaaagggc aattgcgcgg tgggctgctc ttgctgcgga
    11821 acagacacag gctgtgacgc tgcgttgttg gcgaccactt cgcttaacgg aacggttttg
    11881 gtttctggcg gataacagaa accggcatca gcacagccct ggtaggtgac agttaacgtc
    11941 gctcccgcac tcgcctggtt gatggttacg ggaagcgtca gccgatcgcg gtaaatctcg
    12001 cttttgccgt aaaactcatc ttcatgccag acgccttgcg gcagctgcac gtcggcaatt
    12061 ttcgcgtgtt ccggcgtaat gcggatctgt ttacggtaga ggtagtaacc gtctttgatc
    12121 tgccaggtca gattaaggtc atgttggttt tgctgaaaat caaaagcaaa ggcttgatcc
    12181 acggggacaa attgtgaacg tcccggcgcg tcgaataatc cggcaaaaac ggaagtgctg
    12241 caaagtagca ggatcagcgt aaagatgcgt tgagccatga gaggtaatct gtgtctccgt
    12301 gtgtaacagg taaaaccaga agttccgggg tttgatatgg atgatgagac ttcaggcatt
    12361 ccagcagtgc ctgctggtga gatacggtag tttttaaaat catctgcact tcgtattctt
    12421 gctccagctt accttcccag taatagagag aggtagcgcc ggggatcaag gtcgcgcagg
    12481 ccgccagttt ttccgccagc actttggcgg ctaaatcctg ggctgtcgct tcatccggtg
    12541 cggtacatag caccacgaca gacgcggtat tcgaactttt ttcatcaagc ataaacacct
    12601 cgcaagaaca gatggaaccg caagagagag gtcactatac aacggacggg gaaaggatgt
    12661 tagtcggcga gaaagaaagc ggggcgtgaa cgccccgcga ttggtcatta cagcatgaag
    12721 ctacccagca cgaagccgaa gcaaacggcc agggcaacac ccagagtacc cgggatgaag
    12781 aacggatggt tgaagacgaa tttaccgata cgggtagtac ccgtgtcatc catctgtacc
    12841 gcagcaacca gcgtcgggta ggtcggcaga atgaacagac cagacaccgc agcgaaagaa
    12901 gcaacagcgg tcagcggaga aacgttcagt gccagagcca tcggcatcag tgcttttgcg
    12961 gttgcagcct gagagtacag cagagcagaa gcaaagaaga agatgacggc cagcagccac
    13021 ggatgaccct gaatcacttc accagcggta tctttgatcc agtcgatgtt gttggaaacg
    13081 aaagtatcgc ccagccacgc aacacccagg atacaaatac aggcgctcat acctgctttg
    13141 aaggtgctgg agttgaggat gttgtcggta tcgactttac agataacggt ggtcagagtt
    13201 gcaacactga gcatgatgat caggattgcg ttggtggtgt tcatcagcgg tttttcaacc
    13261 agacccatgc ttgggctgtt gatgattgca tagataacca cgccaactac gcccagcagg
    13321 aacagccaga cggacgtttt tgcaccggat ttgatttcaa tctgcttttc accgcgcagt
    13381 tcaaccaggc cctcttccag acgcttgcga taaatcggat cgtcagagag tttggagttg
    13441 aacagcatag tgaccaggaa ggacatcacc agaaccgcca gcagggtgga cgggatgacc
    13501 acggagagca gatggaggta gctgatgcca tgaccttcca tcacggaaga catgtaaacc
    13561 actgccgctg agattggcga tgcggtgatc gcaatctgcg cggataccac tgcagtagac
    13621 agcggacggc aaggtttaac gccttgttcc ttcgcaactt cagcgataac tggcagtgtc
    13681 gccagagaga tgttgccagt accagcaaag atagtcagga aataggtcac gatcggtgcg
    13741 aggatcgtga tgtatttcgg gttacggcgc agcagctttt ctgtctgatg aaccagatag
    13801 tccagaccgc cagcaacctg catggcagaa atagcggcga taaccgccat gataatggaa
    13861 atgacatcga acgggatgtt accgggttta acgccaatag cggcaagaac cagcaccccc
    13921 aatccgcctg caaaaccaat acctattccc cccaatctgg cgcccaagaa gatcgccagc
    13981 aaaactatga tgagttctac aactagcata ttagccttcc ttgtttttta acaagttgat
    14041 attagattgt tatttttaag ttactgctca caagaaaaaa ggcacgtcat ctgacgtgcc
    14101 ttttttattt gtactaccct gtacgattac tgttcgcttt catcagtata gcgttttgct
    14161 ttgtaagccg ggtgcatcag attctgtacg gagaaaatat cgtcaagttc cgcttcagtc
    14221 aacagaccgc gttccagaac gacttcacgt acactcttac cggtttcggc acagatttta
    14281 cccacgatgt caccgttgtg gtgaccgatg aacgggttca ggtaagtaac gataccgata
    14341 gagttgtaaa cgtaaccttc gcacacttct ttgttagcag tgatgccgtt aatgcatttt
    14401 tccagcaggt tgtagcaagc gttggtcaga atgtgaacgg attcgaacat agcctggcca
    14461 atgaccggct ccataacgtt caactgcagc tgacctgctt ctgctgccat ggtaacagtg
    14521 gtgtcgttac cgatgacttt gaagcatacc tggttaacca cttccggaac aaccgggttt
    14581 actttagctg gcatgatgga agagcccgcc tgcagttccg gcaggttgat ctcgttcagg
    14641 ccggcacgtg gaccagaaga gagcaagcgc aggtcgttac agattttgga catcttcaca
    14701 gccaggcgtt tcagcgctcc gtgaaccata acataagcgc cgcagtcaga ggtcgcttcg
    14761 atcaggtctt cagccggtac gcatgggaag ccagtaactt cagccagttt tttcactgcc
    14821 agcggagagt actctttcgg cgtgttcaga ccagtaccga ttgctgttgc gccaaggtta
    14881 acttccagca gcagttcagc ggtacgttgg atgtttttca cttcttcttt cagcaggatg
    14941 ctgaaagcgc ggaattcctg accgagggtc atcggtactg cgtcctgcag ctgggtacga
    15001 cccattttca ggatgtcctg gaattcgacc gctttacgtt caaagccttc acgcaattgg
    15061 ttaatcgcat ctaccagctt aatcagggaa gagtaaactg cgatacggaa accggtcggg
    15121 taggcgtcgt tagtggactg acatttgtta acatggtcgt tcgggttcag gtactgatat
    15181 tcacctttct ggtgacccat cagttccaga ccgatattgg ccagcacttc gttggtgttc
    15241 atgtttacgg aagtacctgc gccgccctgg tagacgtcta ccgggaactg atccatgcat
    15301 tttccgttgt tcaggacttc atcacatgcg gcaatgatgg cattcgctac acttttagga
    15361 atggtttgca gctctttgtt tgccatagct gcggcttttt taaccattac cataccgcga
    15421 acaaattcag gaatatcact gattttgttg ttgctgatat agaagttttc aatcgctctc
    15481 agagtgtgaa caccatagta ggcatcagct ggaacttccc tggtacccaa cagatcttct
    15541 tcgatacgaa tgttgtttga catgtgaacc ttctttttca agctgccaat gatttgcttt
    15601 aaacacacag aatatatgtg gtttcgaatg tttttcgacc gacgattatc ccctgcatcg
    15661 accgaatatc cgagatcata tgctgcttga ggatttctac cgtaatctgg atcactttaa
    15721 gtgtcggttt ttacccctta attattaatt tgtgaaatag atcaccgctt tgggattact
    15781 accaaaaata gttgcgcaaa tatcttgaaa ttttgctaat gaccacaata taagctgaac
    15841 gcgattcgca acccattcag gtagccgggt ttaaccggct gctattacag gagaaacctt
    15901 tgcgctggtt accttttatt gccattttcc tttatgtcta tattgagatt tcaatcttta
    15961 ttcaggttgc ccatgtattg ggggtattgc tgactctcgt gctggttata tttacgtcag
    16021 ttatcggtat gtcactggta cgtaaccagg gctttaagaa tttcgtgctg atgcagcaaa
    16081 aaatggcggc gggtgaaaac ccagcggcgg agatgattaa aagtgtttcg ctgatcattg
    16141 ccggtttgct gcttttatta ccgggctttt tcaccgactt cctcggtctt ctgcttttat
    16201 tgccgccggt gcaaaagcat ctgacagtga agttgatgcc gcatttgcgc ttttctcgca
    16261 tgccaggcgg cggttttagc gccgggaccg gtggcggtaa tacttttgat ggtgagtacc
    16321 agcgaaagga tgatgaccgc gaccgccttg atcataaaga cgatcgccag gattaatgtc
    16381 gaaacgccgg atgatgtggt tatgccattt tccggcgttt ttcgttttgg cagaaacagc
    16441 cataaccccg ccagcatgat cagcgcatag agacttttcc agccgatcat tgccagtaac
    16501 agaacgcata acagcccgcc aaccaccgcc agtagtcgat aacgaccttg caataattta
    16561 cagcctgcca gcatgcataa cagataaatc ataataaaga tgccattggc ataaataata
    16621 agagcgtcca gattgatctc taaagcatga atcaccaacg tgctcaccac gcagcagccg
    16681 agcaccgcat tgagggcatt attcgggata tggcgagaag agaggcgtgc caggtagtgg
    16741 tcaggattat gttgcgcctg cgaccagacc aggcgggcga agctctgtat ataaatgttg
    16801 agactggcaa agcaggccag atagccaatt acgcaggcaa tccataacgc tcctacaccg
    16861 aacagttgta ctacaatttt tggaagcgat gctgccgcgg ccatttgttc accataggcg
    16921 tcgaagtgta agacgactac cgtacagccc cagtagacta atcctgccag cagcagacca
    16981 atcatcaaag cacgaggaaa atcacgttct ggatttttaa attccgaggc aagatgggca
    17041 aatgcttcca gaccgacaaa acaccagaac atcactgata acgcagcaaa taacccggta
    17101 agttcgatat ttcctggcgc ggggaagggg atactcgcag gtttgatatc gcccgcccac
    17161 cagatagcga caatcagtgc gacgataagt ccggcaataa ctgtttgtag atttgcactg
    17221 gaactggcac cgcgagtacc gatataccac accaacgcca gcgtaccgag ttctgccaac
    17281 aacagttgcc agctatgcca gccaaacatt gcctggccga atccggcagc aatttgtagc
    17341 gcggcaggca aacccacggg aatgaccgat aaaaacagcc agccggtgac tcgctcaagc
    17401 cgcgaaccaa acgccatacc gacgaagtgc gcgacgccgc ctgcgctggg atagtggcga
    17461 cccagaatcg caaacacaat cgcaatcggg aacactaaga taatcaaaat gggccacgcc
    17521 cacaggctgt tattgcccgc taccagtgca gctaacgcag gaacggcaaa cacgccagtg
    17581 cctaataatg acgtcgatag caggccaatg ccctgggcca gccccagttc ttgtttgagt
    17641 ccactcatgg gttgatgtcc gattgcgccc aaattttggg caactgcgta gattttcgat
    17701 ggtagcacaa tcagattcgc ttatgacggc gatgaagaaa ttgcgatgaa atgtgaggtg
    17761 aatcagggtt ttcacccgat tttgtgctga tcagaatttt ttttcttttt cccccttgaa
    17821 ggggcgaagc ctcatcccca tttctctggt caccagccgg gaaaccacgt aagctccggc
    17881 gtcacccata acagatacgg actttctcaa aggagagtta tcaatgaata ttcgtccatt
    17941 gcatgatcgc gtgatcgtca agcgtaaaga agttgaaact aaatctgctg gcggcatcgt
    18001 tctgaccggc tctgcagcgg ctaaatccac ccgcggcgaa gtgctggctg tcggcaatgg
    18061 ccgtatcctt gaaaatggcg aagtgaagcc gctggatgtg aaagttggcg acatcgttat
    18121 tttcaacgat ggctacggtg tgaaatccga gaagatcgac aatgaagaag tgttgatcat
    18181 gtccgaaagc gacattctgg caattgttga agcgtaatcc tcgcacgaca ctgaacatac
    18241 gaatttaagg aataaagata atggcagcta aagacgtaaa attcggtaac gacgctcgtg
    18301 tgaaaatgct gcgcggcgta aacgtactgg cagatgcagt gaaagttacc ctcggtccga
    18361 aaggccgtaa cgtagttctg gataaatctt tcggtgcacc gaccatcacc aaagatggtg
    18421 tttccgttgc tcgtgaaatc gaactggaag acaagttcga aaacatgggt gcgcagatgg
    18481 tgaaagaagt tgcctctaaa gcgaacgacg ctgcaggcga cggtaccacc actgcaaccg
    18541 tactggctca ggctatcatc actgaaggtc tgaaagctgt tgctgcgggc atgaacccga
    18601 tggacctgaa acgtggtatc gacaaagctg ttaccgctgc agttgaagaa ctgaaagcgc
    18661 tgtccgtacc gtgctctgac tctaaagcga ttgctcaggt tggtactatc tccgctaact
    18721 ccgacgaaac cgtaggtaaa ctgatcgctg aagcgatgga caaagtcggt aaagaaggcg
    18781 ttatcaccgt tgaagacggt accggtctgc aggacgaact ggacgtggtt gaaggtatgc
    18841 agttcgaccg tggctacctg tctccttact tcatcaacaa gccggaaact ggcgcagtag
    18901 aactggaaag cccgttcatc ctgctggctg acaagaaaat ctccaacatc cgcgaaatgc
    18961 tgccggttct ggaagccgtt gccaaagcag gcaaaccgct gctgatcatc gctgaagatg
    19021 tagaaggcga agcgctggca actctggttg ttaacaccat gcgtggcatc gtgaaagttg
    19081 ctgcagttaa agctccgggc ttcggcgatc gtcgtaaagc tatgctgcag gatatcgcaa
    19141 ccctgactgg cggtaccgta atctctgaag agatcggtat ggagctggaa aaagcaaccc
    19201 tggaagacct gggtcaggct aaacgcgttg tgatcaacaa agacaccacc accatcatcg
    19261 atggcgtggg cgaagaagct gcaatccagg gccgtgttgc tcagatccgt cagcagattg
    19321 aagaagcaac ttctgactac gaccgtgaaa aactgcagga gcgcgtagcg aaactggcag
    19381 gcggcgttgc agttatcaaa gtaggtgctg ctaccgaagt tgaaatgaaa gagaaaaaag
    19441 cacgcgttga agacgccctg cacgcgaccc gtgctgcggt agaagaaggc gtggttgctg
    19501 gtggtggtgt tgcgctgatc cgcgtagcgt ctaaactggc tgacctgcgt ggtcagaacg
    19561 aagaccagaa cgtgggtatc aaagttgcac tgcgtgcaat ggaagctccg ctgcgtcaga
    19621 tcgtcctgaa ctgcggcgaa gaaccgtctg ttgttgctaa caccgttaaa ggcggcgacg
    19681 gcaactacgg ttacaacgca gcaaccgaag aatacggcaa catgatcgac atgggtatcc
    19741 tggacccaac caaagtaacc cgttctgctc tgcagtacgc ggcttctgtg gctggcctga
    19801 tgatcaccac cgaatgcatg gttaccgacc tgccgaaaaa cgatgcagct gacttaggcg
    19861 ctgctggcgg catgggtggc atgggtggca tgggcggcat gatgtaattg ccctgcacct
    19921 cgcagaaata aacaaacccc cgggcagaaa tgtctggggg tttttctttt ggtcatcttt
    19981 ctagtataag attcagacac ggacgacgcg agtggcgtcc agctcattga ttatggggaa
    20041 taacatgcac gtaaaatact tagcagggat tgtcggtgcc gcgctactga tggcgggttg
    20101 tagctccagc aacgaattga gtgctgccgg tcagagtgta cgcattgtgg acgagcagcc
    20161 aggcgcagag tgccagctga ttggtactgc gacaggtaag caaagcaact ggctttccgg
    20221 gcaacacgga gaagagggcg gttctatgcg cggcgcagca aacgatctgc gcaaccaggc
    20281 ggctgcaatg ggcggtaacg tgatttatgg catcagtagc ccgtcgcagg gaatgttgtc
    20341 cagttttgtc ccgacggata gccagattat cggccaggta tataagtgcc cgaactgatg
    20401 tgatggtgac aaacgtataa ttgccagatg cgcttcgctt atcaggccta cataaggcat
    20461 tgcaactgat tgaatgcttg agaatttgta ggccggataa ggcgtttacg ccgcatccgg
    20521 catgaccaac gatcacgtta tcagtcattt caggctggat ggctccagcc tcgcattagc
    20581 aaagatgaga ttatttcgcc tgtggtgcag ttttgctggt ggattgaaca cgtaagtgat
    20641 gcagcgcgcg taagcattga tcctgggtta gcggttgggc gttattcgcc gctacggtgc
    20701 gaacatagac ctgacatggc acacccacga gttttttcag tcgtgggctg aaatcaagca
    20761 ttcggcgagt gatcgcctct atttctttac ccggcgttgc ttcacgcgtt ttcaccactg
    20821 cgccgctaaa ttgttctttg ccactttcat ctttaaagcg gtaaagcacc gccagataat
    20881 ggtcgaacaa gttatgtttc cattccggtt gggaataggt gtcatattcc agattgccat
    20941 tttgctggca gtcaacgtca tataacggca ggaaatctaa cagtgaggta atacgtaatg
    21001 ccagttcacg catttccgca ttattaattg catgaatgat tgctcgcgcc agcatttcaa
    21061 tctgcaactc attgacgact aactcacatt tgctaccgtc atgtagcgtt aatacgaagg
    21121 taagattttc gccttgatta tcggtaagtt ccagcgtgtt aacccgacga ttaatatcgg
    21181 catttttaag ctcatcaacc agaatttctg ggatgttttc tgccattttc ttaatgactt
    21241 tatcgcgagc ttgctcatac tgaaggcgtg cagcagcatc cagtttatgc ttttgatgca
    21301 gacgactttc cagcgcaatc agcaggtcgc gcagttccat gacagacata aagaacaacg
    21361 attctttatt gcgaggttct ttaattttca gcgccagtgc aatgaaatta ttactcttgc
    21421 gaataacacc ggtattaaca cccttgatgc ttatggccat gtaggttctc cctaaccatt
    21481 tctcaattaa ataattaatt ttaatttata agccagataa atgggcttgg tagtaatagt
    21541 tgttaaaata acataaatag ccgtactcac tctatataaa cagaaaagaa aggttaatta
    21601 ttggtgttgg ttatataaag agtaacaaca gcaatgtata tgaataatta aaggttatgt
    21661 aacgggcaaa gattaaactt ccctgtttaa tcagttactg ctggcgtagc tggagatcca
    21721 gcggcgtttt gctgggttcg ccgccaattt ctcgcgccag tttcggcacc agatatcccg
    21781 acaccagtgt cagcaactca cgcataatct gccgtgcttc gtcatcactc accataaaat
    21841 gcgccgcgcc ctgtacttta tcgagcacat gcaggtaata cggcatcacg cctgcatcga
    21901 acaacgcatt actcaggttt gccagcgttt gtgcgttatc gttcacacca cgtaacagaa
    21961 cgctctggtt cagcaaagta acgcccgccc ggcgcaactt agccatcgcc tgacggaatg
    22021 tttcatctat ctcattggca tggttgatgt gattcaccag caagatttgc agcgtagaat
    22081 gtgaaaagcg ttcaaccagc gcatctgtga tgcgtgccgg gatcacaatc ggcagacgac
    22141 tgtgaatccg cagacgtttg atatgcggga tggcttccag ttgtgtgagc aaccagtcca
    22201 gctcgtgatc tttcgccatc agcggatcgc cgccggagaa aatcatctcg tccagttccg
    22261 gatgcgcagc aacatactca agtgcagttt gccagttacg cttgttgccc tgattttcgg
    22321 cataggggaa gtggcgacgg aagcaatagc ggcaatttac cgcgcagccg cctttgacca
    22381 gcaaaagcgc ccggttgtgg tatttatgca acaaaccagg cactacgctg tgctgttctt
    22441 ccagcgggtc ggtggagaat ccgggcgcga cgacaaactc atcttgcgag gtaagtacct
    22501 gacgcaaaag cggatcgtcc ggattgcctt tctccatgcg atcgataaat gagcggggca
    22561 cacgcagggc aaacagcttt ttggcgttgc gtccggccaa cagtttttcg tccgcgtcta
    22621 tattcaaaag acgcagaagt tcatcaggat cggtcacaac atcggcaagt tgcgttaacc
    22681 aatcttctct ggatggggta tttagggtta caatatgcgc cattttgtgg cttagctacc
    22741 aattaacaaa tttcagaggg ccttatggca acgtactata gcaacgattt tcgtgctggt
    22801 cttaaaatca tgttagacgg cgaaccttac gcggttgaag cgagtgaatt cgtaaaaccg
    22861 ggtaaaggcc aggcatttgc tcgcgttaaa ctgcgtcgtc tgctgaccgg tactcgcgta
    22921 gaaaaaacct tcaaatctac tgattccgct gaaggcgctg atgttgtcga tatgaacctg
    22981 acttacctgt acaacgacgg tgagttctgg cacttcatga acaacgaaac tttcgagcag
    23041 ctgtctgctg acgcaaaagc aattggcgac aacgctaaat ggctgctgga tcaggcagag
    23101 tgtatcgtaa ctctgtggaa tggtcagccg atctccgtta ctccgccgaa cttcgttgaa
    23161 ctggaaatcg ttgataccga tccgggcctg aaaggtgata ccgcaggtac tggcggcaaa
    23221 ccggctaccc tgtctactgg cgctgtggtt aaagttccgc tgttcgtaca aatcggcgaa
    23281 gtcatcaaag tggatacccg ctctggtgaa tacgtctctc gcgtgaagta atgcggttgt
    23341 ggtgcggcct gcaggctgca ccatcactta ttcaggtcag agatgatgaa acgccttatc
    23401 gttcttgttt tgcttgccag cacgctgctc acgggctgta acaccgctcg cggtttcggc
    23461 gaagacatca aacatctcgg caactccatc tcccgcgctg ccagctaatt tttcttctct
    23521 tccgaaaaat catcagattt ccatcatttt tggcgatgtt gtctattatt aatttgctat
    23581 aggcaaacat aaataacatt acctaaaagg aagacgttat ggtgaagaag acaattgcag
    23641 cgatcttttc tgttctggtg ctttcaacag tattaactgc ctgcaacacc acgcgtggcg
    23701 ttggtgaaga catttctgat ggcggtaacg cgatttctgg tgcagcaacg aaagcgcagc
    23761 aataagcaat aacggtacga cagctgtgtc gtgccgtttg ttttttctgc gatagtcaca
    23821 aaggtaatag ttgaaattcc cccgccacct ggcaaaatat ccgttcaacc atcagctttg
    23881 caggacgacc tgcaaacgcg tcttttcacc ggggacggcc ccaattctcc ggagcctgat
    23941 atgtcctgga ttatcttagt tattgctggt ctgctggaag tggtatgggc cgtaggcctg
    24001 aaatataccc acggctttag tcgtttgacg ccgagtgtta ttaccgtgac ggcgatgatt
    24061 gtcagtatgg cgctacttgc ctgggcgatg aaatcgttac cagtagggac ggcttatgcc
    24121 gtgtggacgg gtattggcgc agtcggcgcg gccatcaccg gcattgtgct gctcggtgag
    24181 tccgctaacc cgatgcgtct ggcgagtctg gcgttaatcg tattggggat tattggtctg
    24241 aaactcagca ctcactaact accgggctgc tgtacccaaa taaacttact gacatcaaac
    24301 ccttcccggg tcgcgactgc cagcatctcc tgtttcactt cgtcagaaat ggttggcgtg
    24361 cgggagagta tccacaggta gtcgcggtcc ggtccgcaaa ccagcgcatg gcggtattcc
    24421 cgatcgagtg caataacgtt ataaccgcca tagaaaggac caaagaatga cactttcagc
    24481 gcagcgcgag ttggtgcgcc ggtaaagtac gctttccctt cactctgctg ccacattcct
    24541 ctgtcagggt tatagccttt attaatgaca ttcaggccgc cgtcatcacg caggctgtat
    24601 gttgcggtga ctttttccag tccacgttca aagcggtgat caaaacgggc aatctcatac
    24661 caggtaccga gatagcgttt agcgtcgaaa ttattcacta cggtcacgcc acgcggcggc
    24721 gtaggagaac tgcaggcaac gaccagaaat gcagctgtcg ctgcggcaac gagagggagc
    24781 aggcgcataa atgtttcctt actggttttt tctaagtgta gatgacagca aggaaaagcg
    24841 cagaaaaggt ccgaaaattc ggacccgatg aaattttact gtagagcgtt gagaatctgc
    24901 caggcggcgg cgactctcgc tggattggga tagtttttgt tagccagcat cacgataccc
    24961 agctcttttt ctggaataaa cgcgacatag ctaccaaatc cgccggtcgc ccccgtttta
    25021 tgcacccatg atgctcgtac tgcaggagtt ggaggcgtaa tcgcttttac ggggcgtgct
    25081 gccagtgcaa ttttattgtc actgccgtta acgatgatgt caggatttac cggccagtcc
    25141 agcatttccc agcccagacc ctgatacata tcgccggttt gccagtagcg agattgtgcc
    25201 agttgtatcc cttgctgaag tgttttgtcg ttgatatcac ggggattcat attgcttcgt
    25261 acccagcagg ccatatcttc aatggtcgac ttcacaccat aagtttcagc atctaacgcc
    25321 cctggcgaaa catgaactgc cttgccttcg cgatatcccc aggcgtaatt cttttcttct
    25381 gcgggcggta cattaatcca cgtatggttg agtttgagtg gctggaagac acgagtttgc
    25441 atcgcctgct caaaactcaa accagacggc ttcacagcca gtgcgccgaa caaaccgata
    25501 ctggagttgg catacagacg ttgtgttcct ggagcccatg caggctgcca gttttgatag
    25561 aagcgcagca agtcgcttga ggatttcact tcatccggca cctgcaatgg caggccgcca
    25621 gcagtgtagg ttgcgagatg taatagtgtg atcccattcc actgtttagc ggtaagttca
    25681 ggccagtatt ttgttgcggg atcgcttaac ttgatttccc ctcgagcaat agcgtcgcca
    25741 ccaagcacgc ccgtaaatgt tttgctgacc gaacctaact caaacaacgt ttgctgtgtg
    25801 acgggctgct ttttggcgat gtccgcatac ccccaggtaa agtaataagg tttaccctga
    25861 taaattaccg ccaccgccat accggggatc ttttgttgct ctataagcgg ggtaattgtg
    25921 cgatgcacaa tatcgttgat ttgttgaggg gcagcaaatg tggagcaaga ggcggtaatt
    25981 aataaggcgc agagcgtcgt tttgaacata gggtctggtt tccatacaaa acggcccgcc
    26041 ataggcgggc cggatttaca ttggcgatgc gttagattgt aacgacacca atcagcgtga
    26101 caactgtcag gatagcagcc agaccgtaga aaacccattt gcccgcaggt acgtggattt
    26161 tcagatcgtg catcgcgtgg tgcatacggt gtaaaccaca ccacagcggc agaacgatca
    26221 tcaggaacag gaatacgcga ccaatgaagc tctgcgcgaa cgccagaacg cgctcgtagc
    26281 tcagtgcatc acccggaaac agccccagcg gcagcagaat acccaccagc aggatcatca
    26341 ccggcgcaat aattgcgctc cacataccac cggccccgaa gaggccccag aataccggtt
    26401 cgtcagaacg ctttggattt ggattaatca tctcaggctc cttaccagta cagggcaaca
    26461 aacaggatta cgatggtggc aaccacagtt accgcccaga gacttttgat aattggctct
    26521 ggtcccattt tttcgtcttt tacaatgata ttggccgctt tcggtgccag ttcaaaccag
    26581 gttttggtgt gcagcagagc tgccgccaga gtgatcaggt taatgatcac aataaccggg
    26641 ttttgtaaaa agtcgacgaa tcccgcccag gcttccgggc catttttcag ggcaaacagc
    26701 ccgaaaatca gttcaatgct gaaccacaca gccggaaccg ccgtgccttc gcgcagcatg
    26761 taaaagcgat aaaacggcaa ttttttccac caggtggacg tcattggccg tacatacggt
    26821 ttacgtttag tcgtcatgtt gcactcctta gcgtggtttc agggtcgcga taagaaagtc
    26881 tttcgaactt tctactttgc cctgttgaat ggccgcagcc ggatcgacgt gtttcgggca
    26941 gacttcggag cagtagccca cgaaagtaca gctccatacg ccgttctggc tgttcaactg
    27001 cgccatacgc tccttcttac cgtggtcgcg gctatcttcg ttataacgat gcgccagcgt
    27061 aatggcagcc ggaccgatga actctgggtt caggccaaac tgcgggcacg cggcgtagca
    27121 cagaccacag ttgatgcaac cggagaactg gtgatacttc gccatctgcg ccggggtctg
    27181 gatgttagta ccctgatccg cggtgcggga gttgccgatg atgtacggtt tgatcgcttc
    27241 cagactttcg atgaagtggg tcatatcgac caccagatcg cgttcaatcg ggaagttagc
    27301 taacgcttca accttcatac cgtcggtgta atcacgcagg aaggttttac atgccagttt
    27361 tggcacgttg ttaaccatca tgccgcagga accacaaatc gccatacggc aggaccagcg
    27421 gtagctcagg tccggtgcca ggttgtcttt gatgtagccc agcgcatcca gtaatgaggt
    27481 agttgcgtca taaggcactt catagaatgc gctatgcggt gcggtatcga cttccgggtt
    27541 atagcgcacc acctcaattt tcaggttttt catctcagcc attcgccttc tccttcttat
    27601 tggctgcttc cgccttatcg gctgcatccg cttcgccacc gtaaacgcgt ttagctggcg
    27661 gtagcgtagt aatcttcacg tcgctgtact ccaggcgagt cgtgccatca gcatcgcgga
    27721 aggcgagggt gtgtttgagg aagttgacgt cgtcacgctt ggtgcaacct tcgtccagac
    27781 gctggtgtgc gccgcgggac tctttacgtg ccattgcgga gtgcgccata cattcagcaa
    27841 cgttcagacc gtggcccagt tcaatggtgt agagcaggtc ggtgttgaac acgctggaag
    27901 tgtcggtgat gcgcacgcgc ttgaagcgtt cctgcagctc tgccagcttg tcgatggttt
    27961 tctgcatcag ttccggcgta cggtagatac cgcaaccttc ttccatagcc aggcccattt
    28021 cgtcgcggat cttcgcccag ttttcgccgc catcctggtt aaccagatct ttcagacgtt
    28081 gttcaacgcc agctgcctgc gcttcaattg ccgcttcgtt gccattaccg gcagttgctg
    28141 cacgctctgt cgcttgttca ccggccagac ggccgaagac caccagttcc gccagggagt
    28201 tggagcccag acggtttgca ccgtgcagac caacagagga acattcaccc acggcgaaca
    28261 gacctttaat gcgggtttca cagttctgat cggtttcgat accgcccatg gtgtagtgtg
    28321 cggtcggacg taccggaatc ggttctttaa ccggatcgac gccaacgtac gctttcgcca
    28381 gttcgcagat gaacggcaga cgttcatgca gttttttctc gccgaggtga cgcaggtcga
    28441 gataaaccac atcaccacgc ggcgtggaga tggtgttgcc tttacgccat tcgtgccaga
    28501 aggcctgaga aactttgtcg cgtggaccca gttccatata tttgtttttc ggctcgccca
    28561 gcggagtttc cgggcccatg ccgtaatctt gcagataacg gtagccattt ttgttgacca
    28621 gaataccgcc ttcaccgcgg caaccttcgg tcatcaggat accggaacct ggcagaccgg
    28681 ttggatgata ctgaacgaat tccatgtcac gcagcggaac gccgtggctt agcgccatac
    28741 ccataccgtc accggtaacg atgccgccgt tggtgttgta acgataaacg cgacccgcac
    28801 cgccagtagc cataacgacc gcgttagcac ggatctgcac cagcgtgcct tccatcatgt
    28861 tcattgctac caggccgcga acatgaccat catcaaccag aatatccagc acgaaatgtt
    28921 cgtcaaaacg ctggatctgc gggaattgca gagaggtctg gaacagcgtg tgcagcatat
    28981 ggaagccggt cttatcggcg gcgaaccagg tacgctcgat tttcatgccg ccgaagcgac
    29041 gtacgttgac gctaccatcc gggcgacggc tccatgggca cccccacagt tccagttggg
    29101 tcatttcggt tgggcagtgg tggacgaaat aatccacgac atcctgctca cacaaccagt
    29161 cgccacccgc tactgtatcg tgaaagtgat attcgaagct gtcatgatcc tgcgcgacag
    29221 cggcggagcc cccttctgca gcaacggtat ggctacgcat cgggtatact tttgagatta
    29281 gtgcgatttt tgcatttgga tttgcctgcg cggcagcaat tgcagcacgt aatcccgcgc
    29341 caccggcgcc tacaatggca agatcggctt gaaaggtttg cacgacattc ctccagattg
    29401 tttttatccc acagccccgt acttcagggt aagtgcctga aagttacggt ctgcgaacgc
    29461 tattccactg ctcctttata ggtacaacag tatagtctga tggataagtc tgaaatttga
    29521 cgagatcgat ttttttagtg cgcaagaggg ctaaattatc actgaagatg attaatttaa
    29581 ttactaaacc atcagatcgt gcttttttta gtcactgccc accgttgttt tattcctgcg
    29641 taaaatttgt ctcgccgtcg cgttgcgagt agacttcgtg cccttgtcaa aaactggaga
    29701 tttaactatg agcgaaacgg catcctggca gccgagcgca tccattccta acttattaaa
    29761 acgcgcggcg attatggcgg agatccgtcg tttctttgcc gatcgtggag tgctggaggt
    29821 ggagacgcct tgtatgagcc aggcgacggt aaccgatatt catttggtcc cgtttgagac
    29881 acgtttcgtt ggccccgggc attcgcaggg gatgaatctc tggttaatga ccagcccgga
    29941 ataccatatg aaacgcctgc tggttgccgg ttgcgggccg gtattccagc tgtgccgcag
    30001 tttccgtaat gaagagatgg ggcgttatca caaccctgag ttcactatgc ttgagtggta
    30061 tcgaccgcac tatgatatgt accggttgat gaacgaggtg gacgatctct tacaacaggt
    30121 gctggactgc ccggcagcag aaagcctttc ttatcaacaa gctttcttgc gttatctgga
    30181 aattgacccg ctctctgccg acaaaacgca actgcgggaa gtggcagcga aactggattt
    30241 gagcaatgtt gctgataccg aagaagaccg cgacacgttg ctacaattgc tgtttacctt
    30301 tggcgtagaa ccaaatattg gtaaagaaaa accgaccttt gtgtaccact ttccagccag
    30361 ccaggcatca ctggcgcaaa tcagtaccga agatcatcgg gtcgctgaac gctttgaggt
    30421 ttattataaa ggtattgagc tggcgaatgg tttccatgaa ttgacggatg cccgtgagca
    30481 gcaacaacgc tttgaacaag ataaccgtaa gcgcgcggcg cgcggtttgc cgcagcaccc
    30541 cattgatcag aatctgattg aagccttgaa agtcggtatg cctgactgtt ccggcgtggc
    30601 attaggtgtt gatcgtctgg tgatgttggc gctgggcgcg gagacactgg ctgaagtcat
    30661 cgcctttagc gttgaccggg cataattctg aaaaattact gaattaacag cgaagaatgg
    30721 cgtgataagc gccattctgt agcatatgtt ttcatttttc cgctaaaggc cacaatttaa
    30781 gactggatat ttgatatcat ccaggtatca attctgtatt ggtttttacg ctgcccgctc
    30841 aatgttgggc agtgcagttc agtgggcgcg ttttatacac gcgctgaaat gaaggatggt
    30901 ttcatgcctc acacgataaa aaagatgagt ctgataggac tcatattgat gatctttact
    30961 tccgtatttg gatttgccaa tagcccatcg gcttattact taatgggtta tagtgcgatt
    31021 cccttttata tattttctgc attgttattc tttattccat tcgccttaat gatggctgaa
    31081 atgggagctg cttatcgcaa agaagaaggc ggtatctatt cctggatgaa taatagtgtc
    31141 ggaccacgtt ttgccttcat tggtacgttt atgtggtttt cctcttatat catctggatg
    31201 gtgagcacct ccgcgaaagt ttgggtaccg ttctcaacat tcctctatgg tagcgacatg
    31261 acccagcact ggcgtattgc cggactggag cctacgcagg tggttggtct gctggcagtg
    31321 gcatggatga ttctggtcac cgtcgttgct tcaaagggga ttaataaaat tgcccgcatt
    31381 actgcggtgg gcggtattgc agtaatgtgt ctgaatttag tattgctgtt agtaagcatt
    31441 actattttgt tattaaatgg tgggcatttc gcgcaggata ttaatttcct tgcatcaccg
    31501 aacccaggtt atcagtccgg tctggcaatg ctatcgtttg tggtatttgc tatttttgcc
    31561 tatggcggaa ttgaagcggt tggtggtctg gtcgataaaa cggaaaatcc agaaaagaac
    31621 tttgccaaag gtattgtttt tgccgctatt gttatttcaa tcggttattc gctggcaata
    31681 tttttatggg gcgtcagcac aaactggcag caggtattaa gtaatggttc cgttaacctc
    31741 ggcaatatta cctatgtgct gatgaagagc ctcggggtga cgctgggtaa cgcactgcat
    31801 ttgtcacctg aagcgtcatt gtcgctgggc gtatggtttg cgcgtattac cggactttcg
    31861 atgttcctcg cttataccgg tgcgttcttt acgctttgct attcaccgct gaaagccatc
    31921 atccagggga cgccgaaagc gttgtggccg gaaccgatga cgcgcctgaa tgcgatgggg
    31981 atgccgtcta tcgctatgtg gatgcagtgc gggttggtta ctgtcttcat cctgctggtt
    32041 tcgtttggtg gcggtaccgc atcggcgttc tttaacaagc tgacgctgat ggcgaacgtg
    32101 tctatgacgc ttccttacct gttcctcgcg ctggctttcc cattctttaa agcacgtcag
    32161 gatctcgaca gaccatttgt gattttcaaa acgcgtatgt cggcaatgat tgcgacggtg
    32221 gttgtcgttc tggtggtgac atttgcgaac gtcttcacca ttattcagcc tgtggttgaa
    32281 gccggagact gggacagcac attgtggatg attggcggcc ctgtcttctt ctcgctgtta
    32341 gcgatggcga tttaccagaa ctattgcagt cgcatggcga ataaacctga gttagctctc
    32401 gactgatcat atgaaatcct gcaactcatc gtctctccgg cgatgagttg tcaggaagta
    32461 atgcgtatgg atgatactct ccagggccgg tgataactga agatgatgtt aagctgctaa
    32521 atgagccacg ttgacatcat ttggcagctt catgcattac aaacttcccg cctgacgacc
    32581 tttgcccgca gacgtcagcg ttctccccgt ttgtttaccg ttgaggcttt ccagacgcat
    32641 ctggaagggc gggaatggca tatcgatacc gtgggcatgg aagccagcca gaatcagctg
    32701 gtggatctca tggcgtagcg gcatacggtg acccatctca gcggcgtaaa tacgcagctc
    32761 gaaaatctga atcccctgtt gcagatccac caggaagact tccggtgccg ggttgtcgat
    32821 caccagcgag cagcgacgcg ctgcggtgag caggatttcc gtcacttctt cactgttggc
    32881 atcggcaggg gccggaatcg tcaacaccac gcgcgtgacc gagtcagaga gcgaccagtt
    32941 gataaactgc tcggtaataa acgccttgtt cggcacgatt atctctttac ggtcccagtc
    33001 gctgatggtg gttgcgcggg tattaatttt cgtcacgcta ccagtaagat cgcgaattgt
    33061 caccgtatcg ccaatgcgaa tcggtttttc gaacaggatg atcaggccag agataaagtt
    33121 ggcgaaaatt tcctgcaaac caaaaccgag accaacaccg agcgcggcga ccagccactg
    33181 caatttcgac cactcaatac caatcatcga gaagccgacc agcccgccaa tcagcatcag
    33241 cagatatttg gtgatggtgg tgatggcgta gccggtaccc ggcgttaaat ccaggtgctg
    33301 caaaatcgcc agttccagca gcgcgggcag attgcgcacc agctgcgtgg tgatgataaa
    33361 caccagaatg gcaatcagca ccgcaccgag ggtaatcggc tccagacttt ctacgccctg
    33421 taccgtggag gtgacatccc acagcgaaat attttcgagg aagccgaaag cggaatggat
    33481 ttctgaccac agcacaatga ccgaaagcag ggcgatcaac atcaaaattg agcgcactag
    33541 ccgcaaggac tgcgcactga tggcatcgag atcgacttcg ctttcatcga cttcaattgc
    33601 cccttccggg ctactgtgat gatgtgcttc ctcttcgcca cgcgcacgtt gcgctaacat
    33661 ctctgcccgg cgatgcttcg cccggtcaaa cgccagcctg cgtcgttgga tcagcatcca
    33721 gcggcggata acgtgataaa ccaccagtag caggaaccag atggcaaccg aggtttcaag
    33781 cctcgctaac agcgcctgtg ccgttgccag ataacccacc gccgacgcca gaatggcaac
    33841 cagtggcgcg ccaatcatca tgttccacaa catatggttg gtaatattgt cgccgctgcc
    33901 ttctttgttg agatacagcg ggatcccggc ctttttcagg ctgagggtga ccaccgccag
    33961 cgcaccgcaa atgaggataa agcaaagccg tcccagcgaa ccggaaaatt cgcggtcgtc
    34021 gaggttatcg aacatcatca gcgccataat cagtggcaca ataagcccga tgctcatcag
    34081 gtagtagcgc atcccacgag aaacacgttc gcgcggccag ccaaaatgag cgataaacaa
    34141 gccgttcggg cgggcaaagg tagcgcaaat catcactacc cacagcagcg gcacggtggc
    34201 tgtgacgcca tcaccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta
    34261 acccagcgtc atccacagca ccggcagcgg tgacgcgacg agaatcgacc agaaaagagt
    34321 gcgcaacgtc agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc
    34381 aagaaaacgg gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc
    34441 aaacagcggc aaaatcgtct ctttgctggt cagcatcatc acactggctt tgcccaactg
    34501 actgaaggtg tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc
    34561 gatggtcatc gggcgcacgt cagaggtcca gaacagatag cggtgggtcg cttcgttcac
    34621 ttctttcagc gcatcttcca gttgcccgtt ggagactttc agcttggtca gttccagcag
    34681 tagcgtatcg ccaccctgca acaatgagtt cagcaactca cgctgagtgc gtagctgtgc
    34741 ttccagaata cggttttgct cagcggtcag cggctgaccg tcggcctgat gaatttgccg
    34801 tagcagcggc tgtttattaa gcagatcctc ataacgtaac cgttgcacac gcaactgcgc
    34861 catttcggta tcaagctgtt gtggtttcgg catttccggc agccgtgcca cctgcgcccg
    34921 caacgcttca ccgagcagat tggacgatcc cagccattgc gactgttcac gcagcgtatt
    34981 caacgcctgc cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag
    35041 atccatccgc tgcgcctgtt gattcagcgc cgccgatagt tcgcggttaa ttttgaattg
    35101 cgcgacgata tctttcggca aatcggcact gttttctgct agtaattcgg tactttccag
    35161 cgcccgctct gcctcaagct ggcgttggct gttcaattga ttacgcaagg cctgcaaata
    35221 cgcatccagt tgctggctct ctttttcagc cagctcagag cgcaagcgcg ctaattcctg
    35281 gcggttattg gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc
    35341 agagtcagac tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt
    35401 ccccaggcgg cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag
    35461 ttgattcagc gaatcggcaa tctcgcgggc gcgctcctgc tcttgctggg cctgacggct
    35521 tttatccagc aactgactgc tgacctggag aatttcctga ttcagcgcgt cggtagacat
    35581 ccccggcgac acgctgcgcg gctcgtcacg catgttgttt aattgtgcgc gcagagtagc
    35641 ggagagtttc ggataattat caatgacttc ttgatattgc ttgatgcgct caagggaacc
    35701 ttttcgttcc tcaagcgcat ttaaggcaga ctggagtgcc tctacgactt ctggctgcgc
    35761 gggtttcgcc gcttttgcct gctccagttc ctgggtgatt tgtttgctat cgggggccgt
    35821 cgcggcgtac gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac
    35881 gtcagcgttt cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc
    35941 gtcgtgttct gcttcgatct cttcagcagg aagcggggcg ggttcagcgt ctggcgtaac
    36001 aaaggtttcg gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag
    36061 ttgctcaacc agattcactt tacccggtgc aaacaggttg ataacggtag agccaagttt
    36121 aaagcgaccc atttcctggc ctttcagcag cgccacagaa ccgtcgtttt ccccggcagg
    36181 ccaggtccag cgcttgatga tgccttcgcg cggcggcgta atggtgcccg cccagaccgt
    36241 ctcaatgctg cctacaatcg tcgccccgac cagaatctgc gccattgggc caaattcggt
    36301 atcgaacagg cagatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagccgt
    36361 gagatggtta acggagaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt
    36421 gcacggcatg tgtacgcggt ggtagtcacg cggggagagg taagtggtca caaacgtacc
    36481 gttgcggaac aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt
    36541 gtggcctttg gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc
    36601 atcggcaggc atgaccagga cattcggatc ggtatcgatt gggcgtactt cgtcacgcag
    36661 cggacggaca aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc
    36721 tttcatgtcg accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc
    36781 tgcccgcttg cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta
    36841 ctgtagcgaa agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc
    36901 ctgatccggc ctaaatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct
    36961 taattgtcag tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc
    37021 cagaatacgg tgatagtttt cgaaacgggt ttccgcgatt tttccttcct caaccgcttc
    37081 ccggatagcg cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa
    37141 atagtcatgg aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc
    37201 gaactcacgc acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacaggcg
    37261 tgcggcggtc gtggtgtgct gaccaagccc cgagttgtca gagacatcgt tggtcaggat
    37321 ctctttttgc agccccagca acgcgttcag caggctggat ttgccgacgc cagactgccc
    37381 ggcaaaaatg ctgatgcgcc cggtcaacgc ctcttccagc ggttttagcc catcctgagt
    37441 atggctggaa accatcaata cgcgataacc gatattgcgg tagatatcca tctgttcgtt
    37501 gacgaacgcc atgccttcgt cgtccagcag gtctatcttg ttgagcacaa taatcggctc
    37561 aatctgcaag gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg
    37621 caaaatggcg gagacaatga caatctggtc gatgttggcg gcaataggtt tcacgccgtc
    37681 gtagaaatcc ggacgcgtca acactgaggt gcgctcatgc accgcttcca cgatcccttt
    37741 gacatttacg ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggtcac
    37801 cagcgaacgg atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac
    37861 atcagcgtgc ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt
    37921 gtcgtcgtag tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac
    37981 gcggcgctgc tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc
    38041 cctgatgggc aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg
    38101 ggtggaaaat agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac
    38161 cggtctggat cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa
    38221 tctgaatatt ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc
    38281 gctgatggat ggctggaacg tgcgcaccca taccgccagc gggctggtgg agcgcgtgaa
    38341 agcgagcacg atgggcgatc gtgaagctga actggcaacg ctcgaatttt taaaacagtg
    38401 ggtgcctgcg ggtaaatcgc cgatttgcgg taacagcatc ggtcaggatc gtcgcttcct
    38461 gtttaaatac atgccggagc tggaagccta cttccactac cgttatctcg atgtcagcac
    38521 cctgaaagag ctggcgcgcc gctggaagcc ggaaattctt gatggtttta ccaaacaggg
    38581 gacgcatcag gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga
    38641 gcattttatc aagctgtaaa atttttgctg gtcacgtaag cgccggaatc cacttgcggc
    38701 ggcgtgattt tgacgctaaa ttgaacgtct tgctgataat ttcagcactt gagataaaaa
    38761 ctcaaaaaaa aatttttagg ggggttgcag agggaaagat ttctcgtata atgcgcctcc
    38821 cgtaacgacg cagaaatgcg aaaattacga aagcaaaatt aagtagtacg cgggaatagc
    38881 tcagttggta gagcacgacc ttgccaaggt cggggtcgcg agttcgagtc tcgtttcccg
    38941 ctccaaaatt tgaaaagtgc tgcaaagcac agaccaccca agcgggaata gctcagttgg
    39001 tagagcacga ccttgccaag gtcggggtcg cgagttcgag tctcgtttcc cgctccaaaa
    39061 tttgaaagtg ctgtaaggca cagaccaccc aagcgggaat agctcagttg gtagagcacg
    39121 accttgccaa ggtcggggtc gcga
//
LOCUS       DABSYT020000034        39078 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.034, whole genome shotgun sequence.
ACCESSION   DABSYT020000034 DABSYT020000000
VERSION     DABSYT020000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.034"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            52..1707
                     /gene="aslA"
                     /locus_tag="ID725_RS10830"
                     /old_locus_tag="pgaptmp_002166"
     CDS             52..1707
                     /gene="aslA"
                     /locus_tag="ID725_RS10830"
                     /old_locus_tag="pgaptmp_002166"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HGV7850764.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1875..3110)
                     /gene="aslB"
                     /locus_tag="ID725_RS10835"
                     /old_locus_tag="pgaptmp_002167"
     CDS             complement(1875..3110)
                     /gene="aslB"
                     /locus_tag="ID725_RS10835"
                     /old_locus_tag="pgaptmp_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGV7850765.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(3257..3333)
                     /locus_tag="ID725_RS10840"
                     /old_locus_tag="pgaptmp_002168"
     tRNA            complement(3257..3333)
                     /locus_tag="ID725_RS10840"
                     /old_locus_tag="pgaptmp_002168"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3297..3299),aa:Pro,seq:tgg)
     gene            complement(3376..3462)
                     /locus_tag="ID725_RS10845"
                     /old_locus_tag="pgaptmp_002169"
     tRNA            complement(3376..3462)
                     /locus_tag="ID725_RS10845"
                     /old_locus_tag="pgaptmp_002169"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3426..3428),aa:Leu,seq:cag)
     gene            complement(3483..3558)
                     /locus_tag="ID725_RS10850"
                     /old_locus_tag="pgaptmp_002170"
     tRNA            complement(3483..3558)
                     /locus_tag="ID725_RS10850"
                     /old_locus_tag="pgaptmp_002170"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3523..3525),aa:His,seq:gtg)
     gene            complement(3617..3693)
                     /locus_tag="ID725_RS10855"
                     /old_locus_tag="pgaptmp_002171"
     tRNA            complement(3617..3693)
                     /locus_tag="ID725_RS10855"
                     /old_locus_tag="pgaptmp_002171"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3657..3659),aa:Arg,seq:ccg)
     gene            complement(3796..5181)
                     /gene="yifK"
                     /locus_tag="ID725_RS10860"
                     /old_locus_tag="pgaptmp_002172"
     CDS             complement(3796..5181)
                     /gene="yifK"
                     /locus_tag="ID725_RS10860"
                     /old_locus_tag="pgaptmp_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGV7850766.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5372..6112)
                     /gene="wecG"
                     /locus_tag="ID725_RS10865"
                     /old_locus_tag="pgaptmp_002173"
     CDS             complement(5372..6112)
                     /gene="wecG"
                     /locus_tag="ID725_RS10865"
                     /old_locus_tag="pgaptmp_002173"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGV7850767.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6115..7467)
                     /gene="wzyE"
                     /locus_tag="ID725_RS10870"
                     /old_locus_tag="pgaptmp_002174"
     CDS             complement(6115..7467)
                     /gene="wzyE"
                     /locus_tag="ID725_RS10870"
                     /old_locus_tag="pgaptmp_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGV7850768.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7464..8543)
                     /gene="rffT"
                     /locus_tag="ID725_RS10875"
                     /old_locus_tag="pgaptmp_002175"
                     /gene_synonym="wecF"
     CDS             complement(7464..8543)
                     /gene="rffT"
                     /locus_tag="ID725_RS10875"
                     /old_locus_tag="pgaptmp_002175"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGV7850769.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8540..9790)
                     /gene="wzxE"
                     /locus_tag="ID725_RS10880"
                     /old_locus_tag="pgaptmp_002176"
     CDS             complement(8540..9790)
                     /gene="wzxE"
                     /locus_tag="ID725_RS10880"
                     /old_locus_tag="pgaptmp_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGV7850770.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9792..10922)
                     /gene="rffA"
                     /locus_tag="ID725_RS10885"
                     /old_locus_tag="pgaptmp_002177"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9792..10922)
                     /gene="rffA"
                     /locus_tag="ID725_RS10885"
                     /old_locus_tag="pgaptmp_002177"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGV7850771.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10927..11601)
                     /gene="rffC"
                     /locus_tag="ID725_RS10890"
                     /old_locus_tag="pgaptmp_002178"
                     /gene_synonym="wecD"
     CDS             complement(10927..11601)
                     /gene="rffC"
                     /locus_tag="ID725_RS10890"
                     /old_locus_tag="pgaptmp_002178"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGV7850772.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11579..12460)
                     /gene="rfbA"
                     /locus_tag="ID725_RS10895"
                     /old_locus_tag="pgaptmp_002179"
     CDS             complement(11579..12460)
                     /gene="rfbA"
                     /locus_tag="ID725_RS10895"
                     /old_locus_tag="pgaptmp_002179"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGV7850773.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12479..13546)
                     /gene="rffG"
                     /locus_tag="ID725_RS10900"
                     /old_locus_tag="pgaptmp_002180"
     CDS             complement(12479..13546)
                     /gene="rffG"
                     /locus_tag="ID725_RS10900"
                     /old_locus_tag="pgaptmp_002180"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGV7850774.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(13546..14808)
                     /gene="wecC"
                     /locus_tag="ID725_RS10905"
                     /old_locus_tag="pgaptmp_002181"
     CDS             complement(13546..14808)
                     /gene="wecC"
                     /locus_tag="ID725_RS10905"
                     /old_locus_tag="pgaptmp_002181"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGV7850775.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14805..15935)
                     /gene="wecB"
                     /locus_tag="ID725_RS10910"
                     /old_locus_tag="pgaptmp_002182"
     CDS             complement(14805..15935)
                     /gene="wecB"
                     /locus_tag="ID725_RS10910"
                     /old_locus_tag="pgaptmp_002182"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGV7850776.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15991..17037)
                     /gene="wzzE"
                     /locus_tag="ID725_RS10915"
                     /old_locus_tag="pgaptmp_002183"
     CDS             complement(15991..17037)
                     /gene="wzzE"
                     /locus_tag="ID725_RS10915"
                     /old_locus_tag="pgaptmp_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGV7850777.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17049..18152)
                     /gene="wecA"
                     /locus_tag="ID725_RS10920"
                     /old_locus_tag="pgaptmp_002184"
     CDS             complement(17049..18152)
                     /gene="wecA"
                     /locus_tag="ID725_RS10920"
                     /old_locus_tag="pgaptmp_002184"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGV7850778.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18392..19651)
                     /gene="rho"
                     /locus_tag="ID725_RS10925"
                     /old_locus_tag="pgaptmp_002185"
     CDS             complement(18392..19651)
                     /gene="rho"
                     /locus_tag="ID725_RS10925"
                     /old_locus_tag="pgaptmp_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGV7850779.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19736..19837)
                     /gene="rhoL"
                     /locus_tag="ID725_RS10930"
                     /old_locus_tag="pgaptmp_002186"
     CDS             complement(19736..19837)
                     /gene="rhoL"
                     /locus_tag="ID725_RS10930"
                     /old_locus_tag="pgaptmp_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGV7850780.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19978..20307)
                     /gene="trxA"
                     /locus_tag="ID725_RS10935"
                     /old_locus_tag="pgaptmp_002187"
     CDS             complement(19978..20307)
                     /gene="trxA"
                     /locus_tag="ID725_RS10935"
                     /old_locus_tag="pgaptmp_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGV7850781.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20438..21703
                     /gene="rhlB"
                     /locus_tag="ID725_RS10940"
                     /old_locus_tag="pgaptmp_002188"
     CDS             20438..21703
                     /gene="rhlB"
                     /locus_tag="ID725_RS10940"
                     /old_locus_tag="pgaptmp_002188"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGV7850782.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21839..23323
                     /gene="gppA"
                     /locus_tag="ID725_RS10945"
                     /old_locus_tag="pgaptmp_002189"
     CDS             21839..23323
                     /gene="gppA"
                     /locus_tag="ID725_RS10945"
                     /old_locus_tag="pgaptmp_002189"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGV7850783.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(23370..25391)
                     /gene="rep"
                     /locus_tag="ID725_RS10950"
                     /old_locus_tag="pgaptmp_002190"
     CDS             complement(23370..25391)
                     /gene="rep"
                     /locus_tag="ID725_RS10950"
                     /old_locus_tag="pgaptmp_002190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGV7850784.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25608..26057
                     /locus_tag="ID725_RS10955"
                     /old_locus_tag="pgaptmp_002191"
     CDS             25608..26057
                     /locus_tag="ID725_RS10955"
                     /old_locus_tag="pgaptmp_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGV7850785.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26256..26537
                     /gene="ppiC"
                     /locus_tag="ID725_RS10960"
                     /old_locus_tag="pgaptmp_002192"
     CDS             26256..26537
                     /gene="ppiC"
                     /locus_tag="ID725_RS10960"
                     /old_locus_tag="pgaptmp_002192"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGV7850786.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26583..28058)
                     /gene="ilvC"
                     /locus_tag="ID725_RS10965"
                     /old_locus_tag="pgaptmp_002193"
     CDS             complement(26583..28058)
                     /gene="ilvC"
                     /locus_tag="ID725_RS10965"
                     /old_locus_tag="pgaptmp_002193"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGV7850787.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28208..29101
                     /gene="ilvY"
                     /locus_tag="ID725_RS10970"
                     /old_locus_tag="pgaptmp_002194"
     CDS             28208..29101
                     /gene="ilvY"
                     /locus_tag="ID725_RS10970"
                     /old_locus_tag="pgaptmp_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGV7850788.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(29153..30697)
                     /gene="ilvA"
                     /locus_tag="ID725_RS10975"
                     /old_locus_tag="pgaptmp_002195"
     CDS             complement(29153..30697)
                     /gene="ilvA"
                     /locus_tag="ID725_RS10975"
                     /old_locus_tag="pgaptmp_002195"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGV7850789.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30700..32550)
                     /gene="ilvD"
                     /locus_tag="ID725_RS10980"
                     /old_locus_tag="pgaptmp_002196"
     CDS             complement(30700..32550)
                     /gene="ilvD"
                     /locus_tag="ID725_RS10980"
                     /old_locus_tag="pgaptmp_002196"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGV7850790.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32615..33544)
                     /gene="ilvE"
                     /locus_tag="ID725_RS10985"
                     /old_locus_tag="pgaptmp_002197"
     CDS             complement(32615..33544)
                     /gene="ilvE"
                     /locus_tag="ID725_RS10985"
                     /old_locus_tag="pgaptmp_002197"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGV7850791.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33564..33827)
                     /gene="ilvM"
                     /locus_tag="ID725_RS10990"
                     /old_locus_tag="pgaptmp_002198"
     CDS             complement(33564..33827)
                     /gene="ilvM"
                     /locus_tag="ID725_RS10990"
                     /old_locus_tag="pgaptmp_002198"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGV7850792.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33824..35470)
                     /gene="ilvG"
                     /locus_tag="ID725_RS10995"
                     /old_locus_tag="pgaptmp_002199"
     CDS             complement(33824..35470)
                     /gene="ilvG"
                     /locus_tag="ID725_RS10995"
                     /old_locus_tag="pgaptmp_002199"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGV7850793.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35473..35523)
                     /gene="ilvX"
                     /locus_tag="ID725_RS11000"
                     /old_locus_tag="pgaptmp_002200"
     CDS             complement(35473..35523)
                     /gene="ilvX"
                     /locus_tag="ID725_RS11000"
                     /old_locus_tag="pgaptmp_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGV7850794.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(35610..35708)
                     /gene="ilvL"
                     /locus_tag="ID725_RS11005"
                     /old_locus_tag="pgaptmp_002201"
     CDS             complement(35610..35708)
                     /gene="ilvL"
                     /locus_tag="ID725_RS11005"
                     /old_locus_tag="pgaptmp_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGV7850795.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            36061..37581
                     /gene="yifB"
                     /locus_tag="ID725_RS11010"
                     /old_locus_tag="pgaptmp_002202"
     CDS             36061..37581
                     /gene="yifB"
                     /locus_tag="ID725_RS11010"
                     /old_locus_tag="pgaptmp_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGV7850796.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(37605..37943)
                     /gene="maoP"
                     /locus_tag="ID725_RS11015"
                     /old_locus_tag="pgaptmp_002203"
     CDS             complement(37605..37943)
                     /gene="maoP"
                     /locus_tag="ID725_RS11015"
                     /old_locus_tag="pgaptmp_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGV7850797.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            38062..38901
                     /gene="hdfR"
                     /locus_tag="ID725_RS11020"
                     /old_locus_tag="pgaptmp_002204"
     CDS             38062..38901
                     /gene="hdfR"
                     /locus_tag="ID725_RS11020"
                     /old_locus_tag="pgaptmp_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGV7850798.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(38997..39072)
                     /locus_tag="ID725_RS11025"
                     /old_locus_tag="pgaptmp_002205"
     tRNA            complement(38997..39072)
                     /locus_tag="ID725_RS11025"
                     /old_locus_tag="pgaptmp_002205"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39037..39039),aa:Trp,seq:cca)
BASE COUNT         9555 a        10645 c         9861 g         9017 t
ORIGIN      
        1 caatgcttaa ttacgtcatc attttgttga catgaaaccg gagcattttg catggaattt
       61 tcgttttcac ccaaacttct tgttgttgct gtcgccgccg ctcttcctct catggccagc
      121 gcagcagata ccccgtcaac tgccaccgcg cgcaaaggct ttgccggata cgatcaccca
      181 aaccagtatc tggttaaacc ggcgaccact attgccgaca acatgatgcc agtaatgcag
      241 catccggcgc aggataaaga accccagcag aagctggcag aacttgagaa aaaaaccggt
      301 aagaaaccga atgtggttgt tttcttgctg gacgatgtgg gctggatgga cgtcggtttt
      361 aacggtggcg gcgtggcggt gggtaaccct acaccagata tcgacgccgt tgccagccag
      421 gggctgattt taacttcggc gtattctcaa ccaagctctt ccccaacccg cgccaccatt
      481 ctcaccggac aatactccat ccaccacggc attctgatgc cgccaatgta cgggcaaccg
      541 ggcgggctgc aagggttaac cacgctgccg cagttgctgc acgatcaggg ctacgtcact
      601 caggccatcg ggaaatggca tatgggggaa aacaaagagt cgcagccgca gaacgttggc
      661 tttgatgatt tccgtggctt taactcggtg tctgatatgt acaccgaatg gcgcgacgtt
      721 cacgtcaatc cggaagtggc cctgagtccg gaccgttctg aatacatcaa gcaattacca
      781 tttagcaaag atgatgttca tgcggtgcgc ggcggcgaac aacaggccat tgccgacatt
      841 acgccgaaat atatggaaga tctggatcaa cgctggatgg aatatggcgt taagttcctc
      901 gacaagatgg cgaagagtga taagcctttc ttcctctatt acggcactcg cggctgtcac
      961 ttcgataact acccgaacgc caaatatgcg ggtagctctc cggcacgcac ctcgtatggc
     1021 gactgcatgg tggaaatgaa cgatgtgttc gctaatctgt ataaagcact ggagaaaaac
     1081 ggtcagcttg ataacacgct gattgtcttt acctccgata acggaccgga agccgaagta
     1141 ccgccgcacg gacgcacacc gttccgtggt gcgaaaggat ctacctggga aggcggcgtt
     1201 cgcgtaccga ctttcgttta ctggaaaggc atgatccaac cgcgtaaatc tgacggtatt
     1261 gttgatctgg cagatctctt cccaacagca ctggatctgg cggggcatcc tggtgcgaaa
     1321 gtggcgaatt tagtgccgaa aaccaccttt atcgatggtg tggaccagac atctttcttc
     1381 ctgggaacaa atggtcagtc taaccgtaag gccgagcact acttcctcaa cggtaaactc
     1441 gctgctgtgc gtatggatga attcaagtat cacgtcctga ttcagcaacc ttacgcttat
     1501 acccagagcg gatatcaggg tggattcacc ggcacagtaa tgcaaacagc cggatcgtcg
     1561 gtgtttaacc tctacaccga tccgcaggag agcgactcta tcggcgtgcg ccatattccg
     1621 atgggtgtac cgctacagac cgaaatgcac gcgtatatgg agatcctgaa aaagtatcca
     1681 ccacgcgcgc agattaaatc tgattaagcc ggcgctatcg ctgagatctg cctttgccgg
     1741 atgcgatgct gacgcatctt atccagccta cagaacgctg caatttattg aatttgcacg
     1801 atcatgtagg ccggataagg cgtttacgcc gcatccggca atcaaccgca ggcgaccaca
     1861 aaatggccgc ttttttactt actcaccacc agcaaatgcg cctgcataat gtcgctggcc
     1921 gggcgaccgt gcgccagcaa atcagccatt gctttaagat atggcggtag atggcggaaa
     1981 taacgctgat acccggcaca caaataattc agtcccggtt tgccgctggc atcgagcatg
     2041 aagcggtgtt tcgggcagcc tccccaacat gcttttaaca cgttacaact gcgacactgc
     2101 gccggtaatt gcttaaattt atcttcacca aacacctgct gttgcgggga atcgatcatt
     2161 tctgcaattg tttgctggtg catattcccc agtcgatatt gcggataaac atagtgatcg
     2221 caggcgtaaa cgtcgccatt gtgctcaaca atcaccgagc gcccacaggt tggctgatga
     2281 tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg cccattcgat attcatcacg
     2341 aaaatcttgc cgacgtcgcg tttaatccag tggtcgaata tcgccaccag aaattcacca
     2401 aattcatcgg ggcgcacaga ccattccgtc agttccccct gaatatcacc aggcgcatgt
     2461 agtttcagtc cttcgcgggc agtcgtttca tcagccaggc gctcgaccac cggaataaac
     2521 tggatgaatt cgactcccgc atcgcacaaa aaatcataaa cctgtaacgg ttgctgcgcg
     2581 ctggtgcggt tgacgcagac cagcacgtta tagtcgacat gatgtttttg caggagcgtc
     2641 agggcacgca tcaccagttt atgcgtgggt ctgccgcctt ttgtcacgcg atattgattg
     2701 tggatctcag ccggaccatc cagcgataac ccaacaagaa aatgattttc tgccagaaat
     2761 gcacaccatt catcatcgag cagtacgccg ttagtctgga agctgttact tatcttcctg
     2821 ccagcaccat atttcgcctg tagcgccacg gcacggcggt aaaactccag cccgagtagc
     2881 gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt gcggttcgct ggcagcgata
     2941 tagtgacgga catacgcttc cagcgtgtca tcgtccatat gcgtgactgg cttttcgcgg
     3001 taaagggatt gtttttcgag ataaaaacag tagtcacagt tcagattaca atcggaaccg
     3061 ctcggtttcg ccatcacatg aaaagcacgc gttggaacct gttgcagcat ggtcgctcct
     3121 tgcctgaagt catccttcaa tacgttaagg gcgaaatcat accgtggggc aatgagttat
     3181 tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca gaaaacaaaa aaccccgccg
     3241 aagcggggtt caaaattggt cggcgagaga ggattcgaac ctccgaccca ctggtcccaa
     3301 accagttgcg ctaccaagct gcgctactcg ccgaaatact gctttttgaa tttttagttc
     3361 aattctttaa agtcgtggtg cgaggggggg gacttgaacc cccacgtccg taaggacact
     3421 aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcattgtcac aacttctaat
     3481 aatggggtgg ctaatgggat tcgaacccac gacaactgga atcacaatcc agggctctac
     3541 caactgagct atagccacca ctacaaatct tgttacgcgg tattactacc accgcagctc
     3601 aagcgccggg actaaatggc gcgcccgaca ggattcgaac ctgagacctc tgcctccgga
     3661 gggcagcgct ctatccagct gagctacggg cgcttagcgc cgttgcgggg gtggataata
     3721 cggacttccc ccccctctgt ctagtccctt tttaaataaa ttgcgcgttt ggttacggtt
     3781 tgtgcgtttt gctgcttatt cctccagttt atgcgctttc ccgtggcgat taaggccaaa
     3841 aactttataa atcgccgtca ccgccagcat aaagatgatg ccaacaaaca gcgacatacg
     3901 cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc aggaatgcca ttgttacgta
     3961 gttggcccac gggaacagga ttgagcggaa cggatggctg gcaatcgccg ctttatgtgc
     4021 acgccgaaaa cgcagctggc ttatcaatat cacaaaccat ggcaccatcc ccggaagcac
     4081 gctggcactg tagacgtaga caaacacacg ctgcggattg ggaatgatgt agttcaggca
     4141 tgagccaatt agcagaatag caatagatac tgccacaccc gcaaccggta cgccgtgacg
     4201 ggaaactttc gccatcgccg ccggtaactg acggtttttc gccagtgcgt agagcatacg
     4261 tccgcaactg tacatgccgc tgttacagcc agagagcgca gccgtcagca ccacaaagtt
     4321 gataatgccc gccgctgcgg taataccgat tttggcaaaa gtcagtacga acgggctgcc
     4381 gttgctgcct atttcattcc acgggaagat ggtgacgata acgaaaatcg cgccaacgta
     4441 gaaaatcagg atccgccaca gcaccttgcc tacggcactg cgcagcgtca cctgcggatt
     4501 cttcgcttca ccggcagtaa tgccaatcag ctccacgccc tggtaggacg ccaccacaat
     4561 acacagcgcg gtcaggaacc ctttccagcc acccgcaaag aaaccgccat gctctgtgag
     4621 attgctaaaa ccaatcgact gcccgccatt gccaaagcca aagaaaatca cgcccaggcc
     4681 aatgacaatc atcacgataa tcgtggtgac tttgatcatc gcgaaccaga actcgatttc
     4741 gccgtacaac cgcaccgctg ccagattcgc caacgccacc agcgccactg cgatcaatgc
     4801 gggtatccac tgcgccatct ccgggaacca gaactggaca taaacgccaa tggcggtgat
     4861 ttcagagatc cccaccgcca tccacataaa ccagtaagac caggcggtga gatagccaaa
     4921 gaacgggctc atataacgat gcgcataaac ggcgaacgaa ccggtaaccg gttcgaggaa
     4981 caacatttcg cccattgaac gcatgatgaa aaagacgaac agcccggcga tgatataggc
     5041 caacaatacg gatggcccgg cccatttcag ggtactggcg gcccccataa acaggccgac
     5101 gccaatggtg ccccccaggg cgatgagttc gatatgtcga gcttccagcc cacgctgtag
     5161 ctctggtttg ttatctgcca taaatcctcg tgttgtgttt gcatgctttc cggtgttacc
     5221 ggttatcgtt atgggtacat cgagtgttgc gaatgttttc gtaattcagg agaaatggca
     5281 aataaagcat taaaaatttg aatgctttgt gtaataaaaa agcagacagg cgacggagtg
     5341 accactccgt cgctttacaa agagaggaaa atcagaggtt gccggtgtaa tgccagcgta
     5401 aataacgcag caaacgaagc tgacgcttaa tgcggctcgg ctgcgaaagc aggcggtaga
     5461 gccactccag ccccagcgtt tgccagattt tcggtgcgcg ttttacgtga ccggtgaaaa
     5521 catcgtaagt cccgccaacg cccatataca gcgcatctgg atgcaccaga cggcagtcgc
     5581 gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt gacgatttgc gctccgctgg
     5641 catgaatgcg ttcaaacagc gcctgacgct gctcgggttt aaaataacca tcctgactgc
     5701 caacgatatt cacattccac tggttgcgca gtttagcttc agtttgcgcc agcacttcag
     5761 gtttaccgcc cacaagaaat accgtcgtcc cttctttgcc tgcgcgcgcc atcagctctt
     5821 cccagagatc ggcaccggca acgcgggaaa cctgcgcctg cgggtacttt ttacgtactg
     5881 aacgtacaac gctgatgcca tccgcatatt taaattcggc agcgttaatt aactccctga
     5941 cctcggcgtt ctcttcaatg gtcagcattt tttcagcatt aatggcaacc agcgttccct
     6001 gcttaagctg cccgtcagca aacagataat cgagggcatg ctgcatatca cgccaaccaa
     6061 tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt gttgttattc attgttatcc
     6121 ttcaacctgc gtccggagcg atgattttgt acgtttatga atgagtccgg cgctttcaaa
     6181 aagccagtac aacagttttg cgatcatcag acatgcgccg aagaccacga taaaaaagac
     6241 cacgcgtgag acaaacgaat ccagcccttc acgcgccagc acgatcatat tgaagatcgc
     6301 cccaaagcag aaactgtgca atatcgcagc cttatagcga ttagtttcgc ggttgcccag
     6361 ctcatacagc cagtcgaacc atttgatgat cagtccaacc acaatcgccc cgagcgggat
     6421 gaacaacgcg ccgcccatca ccactagtga gcctataagc gtaggcgaga tcgccagtcc
     6481 ggagtggtta ttcagcactt cccaggtaaa gtagttggct gagttcagca ccatactcgg
     6541 gcgacccggc cacagccagg aagggataaa gacatagaaa tcgcggacaa ttggcgccag
     6601 gccctggaag tcgatgttgt cgtagttctg caacagcaac gccagattct cccacggcga
     6661 gaaggtgtcg cgagtgagat agagaaacgt atagaacgct tcatcgccgc tcacattcat
     6721 tccatagcgt tttagcgcca gccagaacat gccaacaatc cccagcacgc ccgccgccgc
     6781 cagcatccac aacgaaatcc agccgcgaat aatgccaata aacaggaaga tagcgaatgc
     6841 gatgatgata ttggcgcgag tgccgccgac aatcatataa gtcagcaagc caaaggcgac
     6901 cgtgctgacg aggaaaaaca gccacgcttt gctgtcctgg cgcagaaagt agaccaccag
     6961 catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc acgccggaga cttcactgga
     7021 aaagatctga ctgtaggagt taagccggaa cagcaaaaag ccgttgtgca tgaagaagat
     7081 gccgacgctt accagcgcga tacccatcag gatcacccac gtaagattgg tttccacgcg
     7141 gttcatggta aacagcggac ggcgcggtac atcagcaacg cgtttgcgta ggcgggtttt
     7201 gtaggtgacg tagtaaaccg cgtagaagca gcccgcagaa agcaacgcct gcaacaggat
     7261 ttctggcggc gcgacaccaa catcaaagcg aaacaccagc acgctggtca gcgggaagcc
     7321 aaagaaaaag gtgagcagaa acagcaatga aaagaataca ttgaagttaa agcgcacacg
     7381 acgaaactca aaccaggtca gcgtggcaat aaacagcgtg cagagcagcc agacaacaaa
     7441 caggccactg aattgcagca gactcatgcg acctccccgg cggcaatcgc caacgcccgc
     7501 tgccagcctt gtagatagtt agggctaaag aaggcaatgg tgtttttatc caccgacgcc
     7561 aactggcgct gcgcttcacg cacaatatcc tcgttgagat cgtcggtagt aaacagcacc
     7621 ggtaaatgct gttccgtcat atcctgccag aacggatttt cccggttaag cacacaagga
     7681 atgcccgcct gaatcagtaa gcacagcgta ccaatgccct gctggcgggc aaaaataaag
     7741 taaccaagat cgcactgacg aagtagcgtc agataggcgt caaattccag tttttcactc
     7801 agaacttgca gattttcttc gctgaataac tccagccccg cctgacgaac ttcctcgatg
     7861 tacgcttcgt tatgaggcgg atatcccatc ggcaccacca cttttaccgt atcgccaaat
     7921 tgctgatgaa cggcgcgcaa ggcagcaata tgctcattgc tgcggtcgcc ggagttcccc
     7981 accagaatgg tcattttccc ttcacgttgc cgatcgttcg ccatcgtatt gagagaagca
     8041 tccatccgcg tcgggaagta cagcagttcg ccccgcacct ttgggtgcgt tttggcaaaa
     8101 aagctcaaat caccacgggt ggcaaataca cagccgactc gcttttgcgc caggcgacgt
     8161 agtgggtaaa aaagcttata tctcaagcca ctggaaagct cgtacaggtc tgccccccag
     8221 atatgccagt aaaactggct gggcttaatg ccaccactca gcagagccag ccacagtgtg
     8281 ggattgaact gaccgtggaa gaaaaaacgc tgctgacggt tagcttttgc tttcgcgatg
     8341 accgcttccg ccagcgattt tttcccaggg aaaaattgca cagaaagcgc cggacagcta
     8401 tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc gcgcgtgctc gctcgtcgcg
     8461 gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat ggtgagggat atccgatccc
     8521 agtacgtgaa tcagtacagt catgcccgcc tacgccagag taaaaacacg ccacaacaaa
     8581 gagaaaaata gacgatatag gttgccatat atgcctgtgc cgcgcccagc gcgccgtgcg
     8641 tagggattag ccagtgggca aataccatca ataaagtgaa ctggctgact tccgccagaa
     8701 tataaaaccg cagtgaagct ttggcgatca ccagataacc aaagacataa gcgcccactt
     8761 ttaacacatc acccaccaac tgccaggcaa agagatcgcg catagcggta aatttattcg
     8821 acaacagcag ccagatagca aaatcacgca gcaaccagac ggtaaaactc gccgccgcca
     8881 ctgccggtaa gacgaatttc agcgatttaa ccacttcccg ggtgatatcg cgcttttccg
     8941 ttagccgcga caacgtgggc agcaaatata cgctgaacga tgccgtaata aattgcaggt
     9001 aggcatcgga aatactgctc accccttgcc agatccccac ctcatcccag ctatactgcg
     9061 ccgccagcag tttacgcatc atgatgtaag caacaggcaa ggtcaccgac gtaatcaacg
     9121 tcatgagcgt aaatttgctc aactgccctg ccagaccgtt atcccagctg ggtttcagat
     9181 agcttaacgg gatagcacca cgcttaatta acatgacggc ggcaggaatc accaccagcg
     9241 cgggaatcaa cgccagaccc agcaacgccc cttcatatcc gcccaaacgg taactgacgt
     9301 agtacgcaag aacgccaatc aagctgccaa caatcagaga taacgcatta cctgcggcat
     9361 cgcgaaagcc tttcatcaac gccagtaaca ggttgcccca ggcgatcccc atttgcacca
     9421 gcgccactaa acgcaccagc ccctgatagt cgtgattacc aaacaatccc aagctgattg
     9481 gcgcagctgc cagcacaaaa accagcgcca tcagcgtaga gaagccaagt accatcgctg
     9541 atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc atggtactgg gcaacgtatt
     9601 tggttacacc gttaaagatg ccagccccgg caagcacgcc gagcacggta atcaactggc
     9661 ggaaatttgc cgccagccca agccccgccg gaccaaatga caccgccagc aacttaccga
     9721 ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc cgtccacaag gacgcttttg
     9781 ccaacgacat atcaggagaa gtagttcaac aaagtcgcaa ttaccgtacg ctgattgacg
     9841 ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct cgctctcttt ggtggtgtag
     9901 cgatcttcac cgtggaactc accaaagcgt tcccccgcag ggcaaccgtg cagcggaatg
     9961 taatggaaca ccgccatgat ttccgcttct ttcagaaagt taatcaacgc gctccggtca
    10021 ccaatatccc gcagtttaat gtaaaacata tgcgcgttct gcaagcagcc atcgggaatc
    10081 gacggcagct cgatacgccc ggctttcgcc agaggtgcta acgcatcgta gtagttttgc
    10141 cacagcgcca gacgttgctg gttgatacga tccgctgctt ccagttgcgc ccacaggtac
    10201 gcagcttgca gatcggacat caaatagctg gagccgatat cgcgccaggt atatttatcg
    10261 acctgaccac ggaagaactg gctgcggttt gtgccttttt cacggatgat ctcggctcgt
    10321 tcgatcaacg ctttatcgtt aatcagcgtc gcgccgcctt caccgcccgc cgtgtagttt
    10381 ttggtttcat ggaagctaaa gcagccaata tgaccaatgg ttcccagtgc acgccctttg
    10441 taagtggaca tcacgccctg agcagcatct tccaccacaa acagattatg ctttttcgcc
    10501 aacgccataa tggtgtccat ttcgcaggcc acgcccgcgt aatgcaccgg cacgataacg
    10561 cgcgttttgt cggtgatcgc cgcttcaatc aacgtttcgt cgatgttcat ggtgtccggg
    10621 cgaacatcca caaaaacgat ttttgcgcca cgcagcacaa aggcattggc ggtggagaca
    10681 aaggtgtagc tcggcatgat cacttcatcg ccaggctgga tatcgagcag cagcgccgcc
    10741 atctccagcg aagcggtgca ggacggcgtc agtaacactt tggcgctgcc aaaacgttgc
    10801 tccagccact gctggcagcg acgggtaaaa ccgccatcgc cacacagttt gccgctaccc
    10861 attgccgact gcatatagtc gagttcggtt cccaccaccg gcggtgcgtt aaatggaatc
    10921 atgtgatcac ctgtataacc agtacgcggt gctttctaca ttcgcaccac tttgtatgta
    10981 tcgtttaagc gcggcggtgt tgcccatttg ggtcgccacc cgcaaagttg ttttaccgcg
    11041 agcatacgcc cagtttagcg ccgtttgcat cagctcagca cctgcaccgc gtccagccag
    11101 caggccaatt cgcgcatctg tagcattgag ttcccgtaaa gagacataac cgcgaatatc
    11161 gccggacgcc gcacgtaaaa tcagacattg atgatcaaag gtgccgcgca cggcattttc
    11221 aatccactgt gcataaaagc gactgctggc gtcaggcgca taccacggcg cacgaaaacg
    11281 gctttgcgca aatgcggcgc tggctaactg acgtaatgcg ggaatatcgg tctcttgtgc
    11341 cactacagca ccgctatcac tggcattgtt cacgggtagc gccaaatcaa cttcaccttc
    11401 taccagggag aatcccagct gttgcagggc atccagttcc cccgtatttg atgccgcaat
    11461 tttggcctgc acccgtgacc acggcgctaa cgcgtctggc gtcaggaacg gtgcttcaga
    11521 cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg ttttcccagg ttagtggctc
    11581 aatactggcg cggacgggca cgaagtaact ccagcagata ttggccgtag ccagttttcg
    11641 ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag ccagccgtta cgccaggcga
    11701 tctcttccag gcaggcaatc ttaaagccct ggcgtttttc caccgtctgt acaaaggtgc
    11761 tggcttcaat caggctgtcg tgagtgccag tgtccagcca ggcaaatccg cgcccgagca
    11821 gttcaacggt caggttgccc gcctcgaggt acatctggtt gatggaggta atctccagtt
    11881 caccacgctc cgacggcttc acctgctttg cgtactccac gactttactg tcgtagaaat
    11941 aaagcccggt caccgcccag tttgatttcg gctgttttgg cttttcttcc agcgagatag
    12001 cgcggaaatt atcgtcaaac tccaccacgc caaagcgttc cgggtccatc acctgatagc
    12061 caaaaaccgt cgccacttcc gtgcgcgccg caacatgacg cagcttcgga ctgaagccct
    12121 gaccgaagaa gatgttatcg cccagcacca gacaagaagg ttcaccatta aggaaggttt
    12181 caccgatgat aaaggcctgc gccagaccgt ccgggctggg ctgttcggca tattccagct
    12241 ggataccgaa ctcactacca tcgcccagca ggcgctggaa ataaccttta tcttccggcg
    12301 tagtgatgat gagaatttcg cggataccgg ccagcatcag caccgacagc ggatagtaaa
    12361 tcattggctt atcgtaaatc ggcaacagtt gcttcgatac gccgcgcgta atcggatgca
    12421 atcgggtacc ggaaccgccc gccaggataa tacctttcat gtttgcctcc gctgaaaatt
    12481 agcctttcag acctaaacgc tcgccctgat agctgccgtc ctgcacctgc ttcctccagc
    12541 tttcattagc cagataccac tgcaccgttt tacgcattcc actttcaaag gtttcctgcg
    12601 gcagccagcc aagttcacgg gcaattttcg aagcatcaat ggcatagcgc agatcatgcc
    12661 ccggacggtc agcgacaaag gtgatcaagt cacgataatg caccacgcct tgcggcttgt
    12721 tcggagccag ttcttccagc agctcgcaaa tggtttccac aacatcgaga ttcttacgct
    12781 cgttgtgacc accaatatta taggtttcac cgactttccc ggtggtcgcc acgcaataca
    12841 gcgcgcgggc gtgatcttca acatacagcc agtcacggat ttgctgcccg ttgccatata
    12901 ccggtagcgg tttacccgcc agcgcgttga ggatcatcag cgggatcagt ttttccggaa
    12961 agtggtaagg accgtagtta ttcgagcagt tggtgataag cgttggcaga ccataggttc
    13021 gcagccaggc gcgcaccaga tggtcgctgc tggcttttga cgcggaataa gggctgctcg
    13081 gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga gtgcaggtca ccatatactt
    13141 cgtcagtgga gatatgatga aaacggaacg ctgatttttt atcttccgtc agcgcattcc
    13201 agtaagcccg cgccgcttca agcaatgtat aagtcccgac aatgttggtt tcaataaacg
    13261 ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc tgccagatgc atgacacagt
    13321 ctggctgatg ctcagtgaat acgcgcgcca gttctgcccg atcgcagata tcaactttct
    13381 caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga catcaggttt ccggcgtagg
    13441 tcagcttatc gaccactacc accgcgtcgc tcgtttcgtt gatgatataa cgcaccagcg
    13501 ccgagccaat aaacccggca ccgcctgtta tcagaatctt tctcatcagc gccagactcc
    13561 tttagcatcg acgacatact gctgatggac attgtcgcca ttgataactt tgaactgact
    13621 atgatcgacc agcatcacca gcacgtctgc cgttgccagc gcctcgtcaa gctgcgccag
    13681 agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg ttaggctcaa caaccagagt
    13741 ttcgccgcta tgccactggg cgatcagttc agcgatttcc atcgccgggc tttcgcgcag
    13801 gtcatcaata ttcggtttaa acgccagacc aaagcaggcg attttcagtt cactggcgcg
    13861 tttatcggta gccgccaggc aatcagccac cgccgctttc acctgatcga taacccagaa
    13921 cggtttgtga tcgttcactt cgcgcgcggt acggataagc cgcgcctgct gggggttctg
    13981 tgccacgata aaccacggat caacagcaat gcagtgaccg cccacgccag ggccaggctg
    14041 aagaatatta acgcgagggt gacgattcgc caggcgaatc agttcccaga cgttaatccc
    14101 ctgatcggca caaatcagcg acaattcatt agcaaaagcg atattcacat cgcggaagct
    14161 gttttcggtg agcttacaca tttccgccgt ccgcgagtta gtgacgacac actcaccttc
    14221 gaggaaaatt ttgtacagtt cgctggcgcg ggccgaacaa accggcgtca taccaccaat
    14281 cacgcgatcg tttttaatca gctcgaccat aacctggccc ggtaacacgc gttccgggca
    14341 gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgt gggaaagtga gatccggacg
    14401 catctctgct aaccattctg ccatcttctc ggttgacccc accggcgagg tggattcaag
    14461 gatcaccagc gcgccttttt tcagcactgg cgcaatggag cgagcagccg attcaacgta
    14521 ggtcatatct ggctcatgat cgcctttaaa cggcgtgggt acagcaatca gccaggcatc
    14581 cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct tctacggcag tttttactac
    14641 gctcgccaaa tcaggctcga cgatatggat ttcgccacga ttgatggtat caaccgcgtg
    14701 ttggttgata tcgacaccaa tcacctgttt ttgccgtgag gcaaacgctg ctgctgtagg
    14761 cagcccgatg taacccagtc cgataacaga aatggtcgca aaactcatag tgatatccga
    14821 ttatttttta acgcttccag aatgcgagag catgcctgac catcaccata cgggttatgg
    14881 gcgcggctca tagtttgata ttcgttttcg tcttttaaaa gacgcgtcac ttcctcgaca
    14941 attcgctgct tatccgtgcc taccagacgc accgtacccg ccgtcaccgc ttccggacgc
    15001 tcagtggtat cgcgcatcac cagcaccggt ttccccagcg aaggcgcttc ttcctgaatg
    15061 ccgcctgagt cggtcaaaat cagccaggcg tggttcatca gccagacaaa cggtaaatac
    15121 tcctgaggat cgatcagaat gacatttttc acatgcccca gaatgcgatt gaccggctct
    15181 ctgacgttcg ggttgagatg caccggatag acaatctgga tgtcctggtg cgtggtggcg
    15241 atgtctgcca gcgcgtggca gatttcttca aagccacgac cgaagctttc acgcctgtga
    15301 ccggtcacca gaatcatctt tttatcgggg tcgataaacg ggtaatttgc cgcaagttct
    15361 gaacgcagcg tgtcgctgct catcacctgg tcacgcaccc ataacagtgc atcaatgact
    15421 gtattaccgg taatgaagat tcggctatct gcaacgtttt cacgcagcaa gttttgccgg
    15481 gaagtttcgg ttggagagaa atgatacatc gccagatgcc cggtcaatgt acggttagcc
    15541 tcttccggcc acggcgaata gagatcgccc gtgcgtaaac cagcctcaac gtgaccaaca
    15601 ggaatacgct gataaaacgc cgccaggctg gttgccagcg tcgtcgttgt gtcgccgtga
    15661 accagcacga cgtctggttt gaactcggca agaataggtt ttagcccttc cagaatccga
    15721 caggttatct ctgtcaggcc ctgtcctggc tgcattatgt tgagatcgta gtcaggtaca
    15781 atggaaaaga gtttcagcac ctgatcgagc atctcccgat gctgcgcagt gacgcaaact
    15841 ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca acggagccat cttgatggct
    15901 tccgggcgcg taccaaatac agtcagtact ttcacatcga ttctcttcga ataagcggcg
    15961 agcgcctttg cgctcaccgc agcagtgttg ctatttcgag caacggcggg ttaatgcgac
    16021 accagccccg atcagccccc cgacaatgcc ccacataatc atcaggaagg cacgacgtgg
    16081 gctatcgcgt tttaccggtt cttccggcgt acgcaaatag cgataggtct gaaaacgcgg
    16141 atccagggtt ggaccaacat tcagggtgtt taacatggcc cgattctgat catagtcgag
    16201 atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga gcctggagca ttggacgccc
    16261 aagcaggaac atttctgaat caggtaattc ctcggcaggc acatctgtcg cactgcgcga
    16321 aatattatgc tgctcagcaa ttttcagcgc ctgctcaatg ctgttcatcc ggcggtcgta
    16381 gatggctttc gccacctctt cctgacgctt cacctgtgct ttcatctgga tggtacgcgc
    16441 cgcccatgcg cctttcagct catcattcag atggctggct gcacgctggc tggcaaaagc
    16501 aacatactga cgtaacaggt tattagcgtc aggcgcggtt tcggcaataa gcttcacgct
    16561 gtcattgacc gcgcgggtaa agtctccggg gataaactgg atgttgttaa tcatttcatc
    16621 cagcaacgcc gcatcggctt tgctgttgcc caccatccgc tgcttgtaat agtcggtttg
    16681 cagccagaac tctctgcggg tatcccacga ggccagttgc ataacaaact ctttataggc
    16741 ttcgtccatg accgatggtt ggtcggcaga agccatgttt gaacggacat ccaggttacg
    16801 caaaaattgc tgctgcgagt aatatccccc cagcatattc accgttggac gatcggtaat
    16861 cgccgtcgag ctccactcct gacgagcaaa aaacgtatac gccagcgcga ttaacgcaaa
    16921 cgccagcccc atgccaataa tccatagctt cccagcccac aaggtacgaa acaacccacg
    16981 aatatccagt tcattttcag cgtcttcggc cggtttccca ggcattggtt gtgtcatcac
    17041 atcctcattt atttggttaa attggggctg ccaccacgat ttctacgcag tctgcgtttt
    17101 acgcgcttaa taaagcgagc aactttccag gcacgcttaa tgcaatatcc atagaggagg
    17161 aatgctagca aaaagagcac caacatgacc cactccggga caaaatgaga atattctgcc
    17221 agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa tcagcacaaa cgcctggcgg
    17281 gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct gacggtcagg agagaatggg
    17341 ctcatgcctt tacgcaggcg acggtacata atcgccacca tatccattag cggaatggcg
    17401 attatccaca aagcggtaac cgggctgatg ggatgggttt tgccttgggt cgtttcgagc
    17461 aggatccaga tcacggtaaa accaatcagc gtactgcccg catcacccat aaagactttg
    17521 tagcggcgac ccaggatacc aaggttaagc atgatatatg gcaggatggc ggcgatcatc
    17581 gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc acaaaatcat accgattgct
    17641 gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa tgccatcaac catgttgaac
    17701 gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt aaccaaacgg tccgagcacc
    17761 atctcccagg agccaaagat ataacccagg ctactgagat aaaggttgcc aaacaccatc
    17821 ataacaatgc caacagcggc ctgtatggtg gcacggattt ttacgctgat atcaaaacgg
    17881 tcatccagcg cgccaatgaa aacaagcaca ccggcacaag cgagatagag agatgcatgc
    17941 ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc ctgcgtaaac cgaaataccc
    18001 ccaacgagtg gtatcaaccc ctggtgacgt ttgcggaagt ttggtttatc cactaaaccg
    18061 actttttttg ccaccttacg ggcaaaaaac agaaacagtg tcgtgaataa aaaaatactg
    18121 atgagatcag tactcactgt cagtaaattc acaatgcatg ctctcagaga aaattattag
    18181 cagaagtata accacgaaga cctttattca gaagggaaag tccgttccga acccaccagt
    18241 cctgtgcaaa aattaaccat ttgatatgca aattgcataa catgccagca aattccagga
    18301 attttcctaa catcgccagc gcggcattaa gattacagat ataaaagcaa aacgccacgt
    18361 aaacacgtgg cgtttttggc ataagacaaa tttatgagcg tttcatcatt tcgaagaaat
    18421 cgtcattggt cttggtcatt gccagtttat taatgaggaa ttccattgca tcgatttcgc
    18481 ccatcgggtg aatgattttg cgcaggatcc acattttctg cagttcttcc tgagtcgtga
    18541 gcagctcttc tttacgggta ccggaacggt tgtagtcgat agccgggaag acgcgttttt
    18601 cagcgatctt acgagagagg tgcagttcca tgttgcctgt acctttaaac tcttcgtaaa
    18661 taacttcgtc cattttagaa ccggtatcga taagcgccgt cgcgataatg gtcaggctgc
    18721 cgccctcttc cacgttacgc gccgcaccga agaagcgttt cggacgatgc agggcgttgg
    18781 catccacacc accggtcaac actttacctg acgccggaac aacggtgttg taagcgcgcg
    18841 ccagacgagt gatggagtcg agcagaatga taacgtcttt cttgtgctca accaggcgtt
    18901 tcgccttctc gatcaccatt tccgcaacct gaacgtggcg agatgcgggt tcgtcaaagg
    18961 tagaagcaac aacttcacct tttaccagtc gctgcatctc ggttacttct tccggacgtt
    19021 cgtcgatcag cagaaccatc agcacacaat ccgggtggtt gtaagcaatg ctctgagcaa
    19081 tgttctgcag cagcatggtt ttaccggctt tcggcggtgc cacaatcaga ccacgctgac
    19141 cacgaccgat aggtgatgcc agatccagta cgcgagcggt taaatcttca gtagaaccgt
    19201 taccacgttc catacgcaga cgagagtttg cgtgcagcgg ggttaagttc tcaaagagga
    19261 ttttgttgcg ggcgttttca ggtttgtcga agttaacttc gttaactttc agcagcgcaa
    19321 aatagcgttc accttctttc ggcgggcgaa tcttaccaga gatggtatca ccagtgcgga
    19381 ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc atcaggaccg gcgaggtagg
    19441 agctgtctgc ggaacggagg aaaccaaatc catcctgcaa tatctccagt acgccatcac
    19501 caaagatatc ttcgccactc tttgcgtgct gcttcaggat agcaaaaata atgtcctgct
    19561 tacgcatacg agccaggttt tccagcccca tattttcgcc gagagtgatc agctcagaaa
    19621 ccggcgtatt ctttaattcg gtaagattca tagtggtgtg agttcttaaa cttggggtaa
    19681 atctcgaact taatgttgtg aatggtatgg caggatcatc catgcctgtt tacggtcatc
    19741 gactcatgtc ttttcgctgc ctggtcacag gagagtacgc agaactgaaa cgacaagacg
    19801 gattgagtga cgagccagaa atctgttcac ttcgcattta agataaaacg ggaagcgttg
    19861 gaaattacaa gattcaaact taataaggta tgtttaatac gaagtcaaca ctaagttagc
    19921 atgactcacg ccgggcgtcc agtttttagc gacggggcac ccgaacatga aattccctta
    19981 cgccaggtta gcgtcgagga actctttcaa ctgaccttta gacagtgcgc ccactttggt
    20041 tgccgccact tcaccgtttt tgaacagcag cagagtcggg ataccacgga tgccatattt
    20101 cggcgcagtg ccagggtttt gatcgatgtt cagttttgca acggtcagtt tgccctgata
    20161 ttcgtcagcg atttcatcca gaatcggggc gatcattttg cacggaccgc accactctgc
    20221 ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca tccgtgtcaa aactgtcgtc
    20281 agtcaggtga ataattttat cgctcatata taactccaca ggaataagcc tggcgtgttg
    20341 gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac tttatttcac cggatacgct
    20401 ttcgtaaagc aatagtaagc tgatattcta ccacactatg agcaaaacac atttaacaga
    20461 acagaagttt tccgacttcg ccctgcatcc gaaggttgta gaagcccttg aaaaaaaagg
    20521 gtttcataac tgtacgccca ttcaggcact ggcccttccg ctgacgctgg cgggtcgtga
    20581 cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg gcgtttctta cgtcaacgtt
    20641 tcattgtctt ctctctcatc ccgcgattgc cgatcgcaag gtgaatcagc cgcgtgcctt
    20701 aattatggca ccgacgcgtg aacttgccgt gcagatccat gccgatgcgg aaccgctggc
    20761 ggaagctacc ggcctgaagc tgggtctggc ttacggtggt gatggttacg acaaacagct
    20821 gaaagtgctg gaaagcggcg ttgacattct gattggcacc acggggcgtt taattgacta
    20881 cgccaagcag aaccacatta acctcggtgc cattcaggta gtggtactgg acgaagccga
    20941 tcgcatgtac gatctgggct ttattaaaga tatccgctgg ctgttccgcc gtatgccgcc
    21001 tgcaaaccag cgcctcaaca tgctgttctc cgccacgctt tcgtaccggg tacgtgaact
    21061 ggcgttcgag cagatgaaca atgccgaata tattgaagtg gaaccggaac agaaaacggg
    21121 ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa gaaaaaatgc gtttgctgca
    21181 aacgctgatc gaagaagagt ggccagacag agcgattatt ttcgccaata ccaaacaccg
    21241 ttgtgaagag atctggggcc acctggcggc agatggtcat cgtgtcggtt tgttgacagg
    21301 cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa tttacccgtg gcgatctgga
    21361 tattctggtt gccaccgacg ttgccgcgcg tggtttgcat attccggcag tgacgcacgt
    21421 ctttaactac gatttacccg atgactgtga agattacgtt caccgtattg gtcgtacagg
    21481 tcgcgcaggc gcaagcggtc actctattag cctggcgtgt gaagagtatg cattgaattt
    21541 gcctgctatt gagacctata ttggtcactc aattccggta agcaaataca atccggacgc
    21601 attgatgacc gatctgccaa aaccgctgcg cctcacgcgc ccgcgcacag gcaatggtcc
    21661 gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt taaataaaaa atgtcatact
    21721 caaaatcatt caaactgtat caagatggca agagaatgaa tcccgatgag cttactgtag
    21781 taagtgattc aggtgaaaga atgcagccaa cacagagaca gattgaagga tgaagagtat
    21841 gggttccacc tcatcgctgt atgcagccat tgatctcggt tcgaatagtt ttcatatgct
    21901 ggttgtgcgc gaggtggctg gaagcatcca gacgctgacg cgaataaaac gcaaagtgcg
    21961 tctggctgct ggcctgaata gcgaaaatgc cctctccaat gaagcaatgg agcgcggttg
    22021 gcaatgtttg cgcctgtttg ctgaacgtct gcaagatatt cctccctcgc aaattcgcgt
    22081 tgtcgctacg gcgacgttac gccttgccgt caatgcgggt gattttattg ccaaagcgca
    22141 ggaaatcctc ggttgtccgg tacaggtgat cagcggtgaa gaggaagcac gtctgattta
    22201 tcagggtgtt gctcacacca ctggcggtgc cgatcagcgc ctggtagtgg atatcggcgg
    22261 tgccagtact gaactggtaa ccggcacggg tgcacaaacc acctcgttgt tcagcctgtc
    22321 gatgggctgc gtcacctggc tggaacgcta ttttgccgat cgtaatctgg ggcaggaaaa
    22381 ttttgatgct gcagaaaaag cggcacgcga agtgttacgt ccggttgccg atgaattacg
    22441 gtatcacggc tggaaagtgt gcgttggtgc ttccggcacc gtgcaggcgt tacaggaaat
    22501 catgatggca caggggatgg atgaacgcat taccctggaa aagttgcagc aattgaaaca
    22561 gcgagccatt cattgcggtc ggctggagga actggagatt gacgggctga cgctggaacg
    22621 tgcgttagtg ttcccgagtg gtctggcgat cctgatcgcc atttttaccg aactgaatat
    22681 tcagtgtatg accctggcgg gcggtgcgct gcgtgaaggc ctggtctacg gcatgttgca
    22741 tctggccgtc gagcaggata ttcgcagccg tacgctgcgt aatattcaac gccgctttat
    22801 gatcgatatt gatcaggcac agcgcgtagc caaagttgcg gctaacttct tcgatcaggt
    22861 ggaaaacgaa tggcatcttg aagcaataag ccgcgatttg ctcatcagcg cctgtcagct
    22921 tcatgaaatc ggcctgagcg ttgacttcaa acaagcgccg caacacgctg cttatctggt
    22981 gcgtaatctg gatcttcccg gttttacccc cgcacagaaa aaattgctgg cgacgctact
    23041 gctcaaccag actaatccgg tcgatctctc atcgctgcat cagcaaaatg ccgtaccgcc
    23101 gcgcgtcgca gaacaactct gccgtttact acgcctggcc atcatttttg ccagccgtcg
    23161 ccgtgacgat ctcgtgccag agatgacatt acaggctaac catgaactgt tgaccttgac
    23221 gcttccgcaa ggttggctaa cccaacatcc gctgggtaaa gagattattg ctcaggaaag
    23281 ccagtggcag agctatgtcc actggccgct ggaagtgcat taatgagtaa gtgccggatg
    23341 cgatgctgac gcatcttttc cggccttgat tatttccctc gttttgccgc catcatcgct
    23401 ttcagattcg ccagatggct ttgccctttc tgcatccgtt cttcggcgct gaccactttg
    23461 cgctcctgtt cccaaatcag atcatcctgc ggcagctcca gcaaaaagcg gctcggctcc
    23521 gggcgcacca gttcgccgta ctgacggcgt tctttacaca gcgtaaaggt caattccttc
    23581 tgggcgcggg taatgccgac ataggccagc cgccgctcct catcgatatt atcttcatcg
    23641 atactgctct ggtgcggcaa aaacccttct tccataccga ccatgtagac ataaggaaac
    23701 tccagtcctt ttgacgcgtg gagagtcatc agttgcacct gatccagctc ttcttcactc
    23761 tcaccacgct ccatcatgtc gcgcaaagta aagcgcgtca ccacctgggt gagcgtcatc
    23821 ggctcatcca gttcgctgcc ttccagcatc tccgtcatcc agctaaacag ttggttgacg
    23881 ttcttcatgc gcatttcggc ggctttcggg ctgggcgatg tttcgtacag ccaggattca
    23941 taatccatgc catggatcag atcacgcacc gcagcaatcg gctcccgctc cgccaggcgc
    24001 tggatttctg ccaaccagtg agtgaagcgg gtcaatgctt cataaccacg tccgctaagc
    24061 gtctgactca ggcccatatc aaagctggcg gtaaacatgc ttttattgcg cgtcatcgcc
    24121 cactcaccca gctttttcag cgtagccggg ccaatctctc gcttcggcgt gttaacgata
    24181 cgcagaaatg cgctgtcatc gtccgggtta gtcagcacgc gcagataagc cagcaagtcc
    24241 ttgatttcag gacgagagaa aaacgacgta ccaccggata ttttgtacgg gatgcggttt
    24301 tgcatcagga atttttcaaa tacccgcgac tgatggttgc cgcgataaag aatggcgtaa
    24361 tctttgtact gcgttttatt gacgaagtga tgggcgatca gctcgccagt aacgcgctcg
    24421 gcttcatgtt cctcgttatt cgcgcttaaa acttttagct ctgtgccgta acccagttcg
    24481 gagaacagac gcttttcaaa gacgtgcggg ttattggcga tcaggatgtt cgccgctttc
    24541 agaatacgcc cggaagagcg atagttctgc tcaagcttaa tcaccttcag cgccggaaaa
    24601 tcctgactca gcagcaccag gttttgcgga cgtgcaccgc gccaggagta gatcgactgg
    24661 tcatcgtcac ccaccacggt aaagcgcgcg cggctgccca ccagcagttt caccagttca
    24721 tactggctgg tgttggtgtc ctgatactca tccaccagca ggtagcgaat tttgttctgc
    24781 cagcgttcgc gtacttcttc attgcgttgc agcagcaacg tcggcaataa aatcaggtca
    24841 tcgaagtcga gaacgttaca ggctttcagg tgtgcatcat acagcccata acaatgggca
    24901 aaaatacgat cccgctcgcc ctttgcctct gccgccgcct gcgccggagt tttgagatcg
    24961 ttcttccagt tagagatggt cgaaatcagt tgttgcagga gaactttgtc atcttcaatc
    25021 agcccctcgg tcaactcttt aagcaaagca agctgatcgg tatcgtcaaa taacgagaag
    25081 ttcgctttca tcccaagcgc cgcatactcg cgtttgatga tatccagccc caacgtatgg
    25141 aaagtggaga tcatcagccc acgcgcctct ttacgcccca gcgtctgccc tacacgctct
    25201 ttcatctcgc gcgctgcttt attagtaaag gtcaccgccg caatgtgccg cgcctgataa
    25261 ccgcaaccgc ggatcagatg ggcgatttta ttggtgataa cacgagtttt accggaaccc
    25321 gcgcccgcca gcaccaggca ggggccggta acgaattcga cagcttgttg ttggccgggg
    25381 tttagacgca taggtgtatt gctcaatctt cgaacggggg gaggattgta gcagaatcgc
    25441 atttgaatgt gaccgcgtgt aagccgctaa ccggttggct gcggatgttt aaatcagaga
    25501 gagaagaccg aaatcggagt tcatcgtgaa tccattagac ttagaaaata tcgggtcgta
    25561 acgttgtgtt attacttttt cagagtttac taaggagagg tttactcgtg gcgattaatt
    25621 tcagccctaa ggttggtgaa atactggaat gcaactttgg taactatcct gttagccaaa
    25681 atggaccgtt cagcacgacg tattacgatg gccgcattcc tcctgaaatg ataaaaaaca
    25741 ggttagttgt cgtgctcaac ggaaaaatta atggcaacgc attcatcgtc gttccattat
    25801 caaccactcg cgaccatgac aagttaaaac gtggaatgca tgttgaaata gccagtaatg
    25861 ttatcaatga cctgcaattc tttgatcagc agattcgttg ggccaaaacc gacctggtac
    25921 aacaagtcag tcgaaacaga ttaaacagag caagaactta cagagggtat cttaatcaat
    25981 gtttgccaca tgagttggtt gcagacatac agagggcggt catcaagtca atcaatgcca
    26041 tatcgctcat aaattgacaa taaaatcacc atccccgata atgactttgt agcccataga
    26101 ggccctaagt tttctcccct tgagggacct gcgaagcccg gattttccgg gcttttgcca
    26161 tatatgtacc agcccctccc tgactgacaa ttacgttagc atcggcggat catgctaaaa
    26221 tcgccgcctg acaattattc actcaaggca cgatcatggc aaaaacagca gcagcactgc
    26281 atatccttgt aaaagaagag aaactggctc tggatcttct cgagcagatt aagaacgggg
    26341 ccgattttgg caagctggcg aagaaacact ccatttgccc atcaggcaaa cgcggcggtg
    26401 atttaggtga attccgccag ggtcagatgg ttccggcgtt cgataaagtt gtgttctctt
    26461 gtccggtact ggagccaacc ggcccgctgc acacccagtt cggttaccac atcattaagg
    26521 tgctgtaccg taactaatag caaggccttc tccgggagaa ggccttgaac tctgaatcgc
    26581 tattaacccg caacagcaat acgtttcata tcagtcatgt agccgcgcag tttcttacct
    26641 acctgctcaa tcgcatggct gcgaatcgct tcgttcaaat cacgcagttg cgcgttatct
    26701 accgcacctt ccggaatagc tttacccaaa tcgcccggtt gcagctctgc cataaacggt
    26761 ttcagcaacg gcacacaagc gtaagagaac agatagttac cgtactcagc ggtatcagag
    26821 ataaccacgt tcatttcgta cagacgctta cgggcgatgg tgttggcaat cagcggcagt
    26881 tcgtgcagtg attcatagta agcagattct tcgatgatgc cggaatcaac catggtttca
    26941 aacgccagct caacgcccgc tttcaccatc gcgatcatca gtacgccttt atcgaagtac
    27001 tcctgctcgc cgattttgcc ttcatactgc ggcgcggttt caaacgcggt tttgccggtc
    27061 tcttcacgcc aggtcagcag tttcttatca tcgttggccc agtccgccat catgccggag
    27121 gagaattcgc cggagatgat gtcgtccata tgtttctgga acagcggtgc catgatctct
    27181 ttcagctgtt ctgacaacgc gtaagcgcgc agtttcgccg ggttagagag acggtccatc
    27241 atcagggtga tgccgccctg tttcagcgct tcggtgatgg tttcccagcc gaactgaatc
    27301 agtttttctg cgtatgccgg atcggtgcct tcttccacca gcttgtcgaa gcacagcaga
    27361 gagccggcct gcaacatacc gcacagaatg gtttgctcgc ccatcaggtc agatttcact
    27421 tccgcaacga aagaggattc cagcacgccc gcacgatgac caccggttgc cgccgcccat
    27481 gctttagcga tcgccatgcc ttcgcctttc ggatcgtttt ccgggtgaac ggcaatcagc
    27541 gtcggtacgc cgaatccacg tttgtactct tcacgtactt cggtgcctgg gcatttcggc
    27601 gcaaccatta ctacggtgat gtctttacgg atctgctcgc ccacttcgac gatgttgaaa
    27661 ccatgcgagt aacccagcgc cgcgccgtct ttcatcagtg gctgtacggt gtgcactaca
    27721 tcagagtgct gcttgtccgg cgtcaggtta accaccagat ccgcctgtgg gatcagttct
    27781 tcgtaagtac ccactttaaa accattttcg gtcgctttac gccaggacgc gcgcttctcg
    27841 gcaatcgctt ctttacgcag agcgtaggag atatcgagac cagaatcacg catgttcagg
    27901 ccctggttca gaccctgtgc gccacagccg acgatgacca cttttttacc ctgaaggtag
    27961 ctcgcgccat cggcgaattc gtcgcggccc ataaagcgac atttgcccaa ctgtgccagt
    28021 tgctggcgca ggttcagtgt attgaagtag ttagccatgg tgattcctcg tgatgttgtg
    28081 cttcttattg ttcggttcgc gtttgcaaga ttgaattcac tatatgacag gaaatttatt
    28141 gcggaaattg atatattcac aacatcatat tgcaattttt gcaacgtcaa catcgagggc
    28201 tgtccctgtg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa gccgccattt
    28261 tggccgcagc gcgcgggcga tgcacgttag cccatccacg ctctcacggc agattcagcg
    28321 cctggaagaa gatctcggtc agccgctgtt tgtgcgcgat aaccgcacgg tgacgctgac
    28381 tgaagcgggc gaagaactgc gcgttttcgc ccagcaaacg ctgttgcagt atcagcagct
    28441 gcgccacacc atcgatcagc aagggccgtc gctctctggc gaattacata tcttctgctc
    28501 ggtgaccgct gcctacagcc atctgccgcc gattctggat cgcttccgcg cggaacaccc
    28561 gtcggtggag attaaactca ctactggtga tgcggcagat gcaatggaaa aagtggtcac
    28621 gggagaagcg gatctggcga ttgccgggaa accggagacg ctacccggcg ctgtagcgtt
    28681 ttcgatgctg gagaatctgg cggtagtgct gattgcgccc gcgctgccct gcccggtgcg
    28741 taatcaggtc tcggtagaga agccggactg gtcaacggta ccgtttatca tggccgatca
    28801 ggggccagta cgccgccgca ttgaactgtg gtttcgacgc aataaaatca gcaacccaat
    28861 gatttacgcc acggttggcg ggcatgaagc gatggtatcg atggtggcgc tcggctgtgg
    28921 tgtggcgctg ttgccggaag tggtgctgga aaacagcccg gagcctgtac gcaaccgggt
    28981 aatgatttta gatcgcagcg atgagaaaac gccgtttgaa ctgggcgtgt gtgcacaaaa
    29041 aaagcggctg catgagccgc tgattgaggc gttctggagg attttgccga accacaaatg
    29101 acgttgtcgc gcgagtaggc ctgataagcg aagcgctatc aggcattttt ccctaacccg
    29161 ccaaaaagaa cctgaacgcc gggttattgg tttcgtcgtg gcaatcgtag cccagctcat
    29221 tcagccgggt ttcgaaatcc ggttcatggt cgccaagttc gaacgccgcc agtacgcgcc
    29281 cgtagtcggt gccatggctg cgatagtgga acaaagaaat gttccagtgc gtacccagcg
    29341 tgttgaggaa gcgcagcagc gcgcccggtg attccgggaa ttcgaagctg tagaggcgtt
    29401 cctgcaacgg atgtgacgga cgcccgccga ccatatagcg cacatgcaac ttcgccattt
    29461 cgtcgtcgga gagatccacc acgctgtagc cgccgtcgtt gagcatctgc aaaatttctt
    29521 tgcgctcttc gaggccgcgg ctcaggcgca cgccgacaaa gatgcaggcg tttttggcat
    29581 cggcaaaacg gtagttgaac tcggtgaccg atcgcccgcc aagcagttgg cagaatttga
    29641 ggaagctgcc tttttcttcc ggaatggtca ccgccaacaa cgcttcacgc tgttcgccca
    29701 gttcgcagcg ttccgagacg tagcgcaggc cgtggaagtt cacgttggca ccggaaagaa
    29761 tatgcgccag ccgttcgccg cgaatgttgt gctgggcgat atattttttc attcccgcca
    29821 gcgccagcgc gccagagggt tccgccaccg cgcgcacatc ttcgaataaa tccttcatcg
    29881 ccgcacagat cgcatcacta tcgacggtga tgatatcgtc gagatactcc tggcacaaac
    29941 ggaaggtttc gtcgccgatg cgttttaccg caacgccttc agcaaacagc cccacgcgcg
    30001 gcagatcaac cggatgaccc gcatccagcg ctgctttcag gcaggcggag tcttccgctt
    30061 ctacggcgat tactttgatt tgcggcatca gttgtttgat cagcaccgcc acgccagcag
    30121 ccagaccgcc gccgccgact ggcacaaata cgcggtcgag atgggcgtcc tgctggagca
    30181 gttccagcgc cagcgtgcct tgcccggcaa tcaccatcgg atggtcgaac ggcggcaccc
    30241 aggtgaaccc ctgctgctgt gacagttcga tcgctttggc tttcgcttca tcaaagttcg
    30301 cgccgtggag cagcacttcg ccgccgaagc cgcgcaccgc gtcgactttg atgtcggcgg
    30361 tggcggttgg cataacgatc agggccttca cgcctaaccg cgcagaagaa aacgcgacgc
    30421 cctgcgcgtg gttacccgca gaagcagtga tcacgccgtg cgctttttgt tcttccgtca
    30481 ggcccgccat catggcgtat gcgccgcgca gcttaaagct gtgcactggc tggcgatctt
    30541 cgcgcttcac cagaatcacg ttatcaagac gagacgagag cttttccatt ttttgtagcg
    30601 gcgtgacctg cgccgcttcg tagaccggcg cgcgcagcac cgctcttaaa tattcagcac
    30661 cttccggggc accggacagg ggttgcgagt cagccattat taacccccca gtttcgattt
    30721 atcgcgcacc gcgcctttgt cggcgctggt tgccaggctg gcataagcac gcagggcaaa
    30781 ggagatctga cgttcacgat ttttcggcgt ccaggctttg tcaccccgag cttcctgcgc
    30841 ttcacgacgc gccgccagtt cggcatcgct tacctgtaac tgaatgccac ggttcggaat
    30901 gtcgatggcg atcaggtcgc cgtcttcaat caggccaatg ctgccgccgc ttgccgcttc
    30961 cggtgagacg tggccgatag aaaggccaga ggtgccgcca gagaaacgac cgtcggtgat
    31021 cagcgcacag gctttaccga gccccattga tttcaggaag ctggttgggt agagcatttc
    31081 ctgcatcccc ggaccacctt ttgggccttc atagcgaatt actaccacat ctccggcgac
    31141 aactttaccg ccgagaatcg cttctaccgc gtcgtcctgg ctttcgtaca ctttcgccgg
    31201 gccggtgaat ttgaggatgc tgtcatcgac gcccgcagtt ttaacgatgc agccgttttc
    31261 cgcgaaatta ccgtagagca ccgccaggcc gccgtctttg ctataggcgt gttccagcga
    31321 gcggatacag ccattggcgc gatcgtcgtc cagcgtatcc caacggcaat cttgcgagaa
    31381 tgcctgggtg gtacggatac ccgcagggcc ggcgcggaac atatttttta ccgcgtcatc
    31441 ctgggtcagc ataacgtcgt attgttccag cgtttgcggc aacgtcaggc caagtacgtt
    31501 tttcacatca cggttcagta accccgcacg atccagctcg ccgagaatac cgataacacc
    31561 accagcacgg tgaacatctt ccatatggta tttttgggtg ctcggcgcga ctttgcataa
    31621 ctgcggaacc ttgcgggaaa gcttatcgat atcactcatg gtgaagtcga tttccgcttc
    31681 ctgcgccgcc gccagcaggt gaagtacggt gttagtcgat ccacccatcg cgatatccag
    31741 cgtcatggca ttttcaaacg ccgctttgct agcaatattg cggggcagtg cactttcgtc
    31801 gttttgctcg tagtaacgtt tggtcaattc aacaatgcgt ttaccagcat tgaggaacag
    31861 ctgcttacgg tcagagtggg ttgccagcag cgagccgttg cccggctgcg acagacccag
    31921 cgcttcggtc aggcagttca ttgagttagc ggtaaacatc ccggagcagg aaccgcaggt
    31981 tgggcatgcg gaacgttcaa cctgatcgct ctgggagtca gatactttcg ggtctgcgcc
    32041 ctggatcatc gcatcaacca gatcgagctt gatgatctga tcggacagtt tggttttccc
    32101 ggcctccatc gggccgccgg aaacaaagat caccggaata ttcagacgca gggaagccat
    32161 cagcatcccc ggggtgattt tgtcacagtt agagatgcag accatggcgt cggcgcagtg
    32221 ggcgttgacc atatactcaa cggaatcagc gatcagttcg cgagatggca gtgaataaag
    32281 catccccccg tggcccatgg caatcccatc atccaccgca atggtgttga actctttggc
    32341 aacgccgcca gccgcttcaa tttgttcggc gaccagttta ccgagatcgc gcagatggac
    32401 gtgacccggt acaaattggg tgaacgagtt cacaaccgcg ataatcggct taccgaaatc
    32461 ggcgtcggtc attccggtgg cgcgccacag cgcacgagca cccgccatat tacgaccatg
    32521 agtggtggtg gcggaacggt acttaggcat actttattta ctcccagtgt ctgtctcgta
    32581 aatgggacgg tgcgtgccgt cccatttttt gtatttattg attaacttga tctaaccagc
    32641 cccatttatc ttcggtttcg ccggtaaaga ggccgaagaa ggcttgctga atgcgtttgg
    32701 tgaccgggcc acaacggcct tcgccaacct gaataccgtc tacactgcgc actggcgtga
    32761 tttctgccgc cgtaccggac ataaagactt catccgccag gtacaaggat tcgcgcgaca
    32821 gcacctgctc acgcacttca attcccagct ctttcgccag tttgatgatg gcatcacggg
    32881 taatacccgg cagcgcggag gaggtgaacg gtggggtgaa cagcacacca tctttcactt
    32941 caaacaggtt ttcgcctgcg ccttcagaga tataaccgtt cacatccagc gcgatacctt
    33001 cctgataacc gtggcggcgc gcttcgctac ccaccagcag ggaagagaga tagttaccac
    33061 cggcttttgc cgcagttggg atggtgtttg gtgctgcgcg gttccaggag gaaaccatcg
    33121 catcgatccc ctgctccagc gcttctgcgc ccagatacgc tccccacggg aaagcggcga
    33181 taatcacatc ggttgagtat cccgctggcg ggtttacgcc catgccaaca tcaccaacga
    33241 agatcagcgg acggatatag gcgctggtga gattgttttt gcggatcacg tcacggcaag
    33301 cttccatcag ctcatcaatg ctctgcgaaa ccgggaagcg atagattttg gcggagtcat
    33361 gcagacgctg catatgctca cgatggcgga atacaaccgg tcctttgtgc gagtcgtagc
    33421 aacggatgcc ttcaaaaacc gaggtgccat agtgcagcgc gtgcgacatc acatgcacct
    33481 tcgcgtcttc ccagcgaacc atctccccat tgaaccaaat gtaatcagct ttcttcgtgg
    33541 tcatttttat attccttttg cgctcaggcg cggatttgtt gtgatgtggt tgtgctctgg
    33601 cagatggcaa cgtgtgcgac gtccaccagt ttatttaact gactaaacag taagtcgacc
    33661 gaccgtgggc tggcaacggt caattcgata tttatatttt gtgcatcgct ggcggcggcc
    33721 atattcattg agcagacgtg gaaaccacga tgacgcacca cgcgtaaaac acgttctaag
    33781 gtttccggat tgaagcgagc cgatacattg acctgatgtt gcatcatgat aatttctcca
    33841 acatttctga attactggca ccaggcggca ccagcggcca gacgttctca agttcgtcga
    33901 ttgagacatg aagcaggtat ggcccatcac tgttcagcat ggtgtcgagt gccgcttcaa
    33961 cctggtcttt acgggtgatg tgttggccag ggatgccgaa ggcgctggct aacttgagga
    34021 aatcggggtt atcggtaagg gtggtttcgc tgtatcgttc ctggaaaaac agttgctgcc
    34081 attgtcgaac catccctaac cgttggttat cgagtaagac gattttcaac ggtaactgct
    34141 tgcgttttac ggtgcccagc tcttgtacat tcatcatgaa agagccgtca ccggagatac
    34201 agacgacggt atcgtttggt cgcgcgactt gcgcgccaac cgccgccggt aaaccaaaac
    34261 ccatggtgcc taagccgctg gaggtaatga aattttccgg gcgagtgtgt gcgatgtgct
    34321 gcgcggccca catctgatgc tgccccacat ctgtggtcac aacgcaatcc gcaggtttac
    34381 gatccgacag ttgttttaac aacaacggcg cgtagatagc gtcaccggga tggtcgtaac
    34441 gccaggcatg ttcatcacgc agctgcgcgc agtattgctg ccagtcattg atatttaatg
    34501 gctgctgtaa tgctggtaac agcgcattta aatcaccctg taatgccaca tgtgcctgac
    34561 gcagcttgtt catttctgcc gggtcgatat ccatatggat aacactggcg tgtggtgcga
    34621 aggtgttcaa tttgccggtc acccggtcat caaaacgtgc acccacggcg atcagcaagt
    34681 cgcactcctg caccgcgaag ttcgccgctt tggtgccatg cattcccagc atgcccagat
    34741 agtacggata atctgcttca actgcgccca gccctttcag cgtgcaggtg gcaggcattt
    34801 ttgtggtagc gagaaattct cgtaaagcag gaactgcctg cgccataccc acgccaccac
    34861 caacgtacag catcggtttt tgcgcttttg ccagcatctg gcgcgcttgc tcaacttcgg
    34921 catgtgggaa agtcacttcg ttttcaacgg tggtgaacca cggttccagg tcgccgctgg
    34981 ctaactggat atcttttggg atatcgacca gaaccggacc aggacgacct gagctggcaa
    35041 cgtcgaatgc ttcagccata atgcgcggca actcttccag cgactgcacc agaaagctgt
    35101 gcttggtaca ggctaacgac aatcccagga catccacttc ctgaaatgcg tcagtaccga
    35161 taaacggtgc ggacacttga ccggtgatgg caacaacagg gatagaatct aacagtgcgt
    35221 ccgcaagccc ggttatcagg ttggttgcgc ccggaccaga cgtggcgata catacgccag
    35281 ttttgccggt agcacgggca taaccgatag ccgccattgc cgcaccctgc tcatgtcggc
    35341 acagcaagtg ctccacgccg ccgtcataca atgcatcgta aaccggcata attgcgccac
    35401 ccggataacc gaaaacggtg ttcacaccct gtgcccgcaa cgcatgtacc acccactgtg
    35461 cgccattcat agttagttcc ccgtcctgaa tcttgggaaa cagaattttg tgctgttatt
    35521 cattgtctgc tcctcggtta tgtttttaag gtcaaaaaaa acccccggac ctttcggtgc
    35581 gggggtctta gttcgttaag gcttgatctc taagcctttc ctcgtccaag tgcagccccg
    35641 cacggtggga taataatcac caccacgcta atcacgacca ggctaatcac tcgtagaagg
    35701 gctgtcattt tgtcttttct tgcatcttgt tcgaaggaat gcctaaagag ttaccataga
    35761 ttttgcgaat agcacaagat attttttggc cgttttttat gatggcttta ataatttcaa
    35821 tagaaattgt tgttttttaa aaagaaaata gtgagtgaaa aaaattcaat ttcagtacga
    35881 atgttgttcc ctccctcgta gttcatcctt tgtccgctgc atttttgcga gcatcattcc
    35941 accgccagaa caactcaact tcacttgcac aaataaacag gaatcagcca ctaaaattca
    36001 cgaattagcg ggctacagca gcgatatctt atgcacataa tggtgactca aggagggctt
    36061 atgtcactgt caattgttca tacccgcgca gccctgggag taaatgcgcc cccaatcact
    36121 gttgaggtac atatcagtaa aggtttgccc ggcttaacga tggtgggctt accagaaaca
    36181 acggtaaaag aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg atatgaatat
    36241 ccggcgaaaa aaatcaccat taaccttgct ccagccgatc taccaaaaga agggggacga
    36301 tatgatttac ctatcgccat tgcgttgctg gcggcctcag aacagcttac agccaataag
    36361 ttagatgaat atgaattagt cggagaactg gcgcttacag gcgctctgcg tggcgttccc
    36421 ggtgcaatct ccagtgcaac tgaagctatt aagtcgggca gaaaaattat cgtcgcgaaa
    36481 gataacgaag atgaagtggg gctaattaac ggtgaaggat gcctggtagc cgatcatctg
    36541 caagctgtct gtgcgtttct ggaaggtaag cacgctctcg aacgcccgaa accaactgat
    36601 gcagtatccc gggcgctaca acatgatctc agtgatgttg tcggtcagga gcaaggaaag
    36661 cgaggactgg aaattaccgc cgctggcggg cacaaccttt tactgattgg gccgccggga
    36721 acaggtaaaa caatgctcgc cagccgtatt aatggtcttt tgccagattt aagcaatgaa
    36781 gaggcactgg agagcgctgc gatattaagt ctggtaaatg ctgaatcagt acaaaaacaa
    36841 tggcggcagc gcccgttccg ctcacctcat cacagtgcgt cgttaacggc gatggtgggc
    36901 ggtggtgcaa ttccagggcc aggtgaaatt tcgttggcgc ataacggcgt gctttttctt
    36961 gatgagctac ctgaatttga acggcgtaca ctggatgctt tgcgagagcc gattgaatcc
    37021 gggcagatcc atctttcacg cacacgagca aaaataacct atccagcccg tttccagctt
    37081 gttgcggcga tgaatcccag ccctaccgga cattatcagg gaaaccataa ccgctgcacg
    37141 ccagaacaga cattacgtta tctcaaccgg ctctcggggc cctttctcga ccgcttcgat
    37201 ctctcactgg agatcccatt accacccccc ggcattttga gtaaaacggt agtgccggga
    37261 gaaagcagca ccaccgttaa acaacgtgta atggctgcca gagagcgcca atttaagcgg
    37321 cagaataagt tgaacgcctg gctggatagt ccggaaatac gcaaattctg caagcttgag
    37381 agcgaagatg cgcagtggct ggaagaaacg ctgatccatc tggggttatc gattcgtgcc
    37441 tggcagcggt tattgaaagt tgcacgaacc attgctgata ttgatcagtc tgacattatc
    37501 acacgtcagc atttgcagga ggcagttagc tatcgtgcga ttgaccgttt gctcatccat
    37561 ctgcaaaaac tactgacata aaaaagggca tttcgccctt tttattaatc gtcagaatcg
    37621 gtgtagtctt cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt
    37681 ggacgcttga tacgcgtcat atacttggac cacacgcgtt ctgcttctgt cactggctca
    37741 cgttcgccac ggcatactgc tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg
    37801 ccaagatcca actcattgaa ggcataacca tgacgctcaa gcagttgtgc ctctttgatg
    37861 gtgaaatcac catggcgaga gaatccacgt ggataatgtt tattgtcgaa atatcgatta
    37921 gtcgtcgtaa agctttccgc catcctgcac gctcctaatt ctttgaccga gctagttatg
    37981 gcgcggagta ttagttacgc ttgacagagt gtaaaacaaa acatttaaat cataacgaca
    38041 aataattttg cggagagcac tgtggatacg gaattgttaa aaactttcct ggaagttagc
    38101 cgaacgcgtc actttggtcg agcggctgaa tcgctctatc tgacccagtc agcagtgagc
    38161 tttcgaatca gacaactgga aaatcaactg ggtgtgaacc ttttcacccg ccacagaaac
    38221 aatatccgtt taaccgctgc cggtgaaaaa ctactgcctt atgcagaaac gctcatgagc
    38281 acctggcagg ccgcccgtaa ggaggtggcg catacctcac gacataacga gttttctatc
    38341 ggtgccagcg cctcattgtg ggaatgtatg cttaatcagt ggctgggacg tttgtatcaa
    38401 aatcaggatg tccatacagg cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg
    38461 gtaaaacagc tgcatgaacg ccagcttgat cttcttatta ccactgaagc gcccaaaatg
    38521 gacgaattta gtagtcagtt gctggggtat ttcactttag cgctttatac cagtgcccct
    38581 tctaaactaa agggagatct taactatctg cgactggagt gggggccaga ttttcaacag
    38641 catgaggcag gtttgatcgg tgctgacgaa gtgcccattc tgacaaccag ttctgctgaa
    38701 ctggcacagc aacagattgc gatgcttaat ggttgcacct ggctacccgt cagctgggcg
    38761 cgtaaaaaag gcggcctgca taccgttgtc gatagcacaa cactttcacg gccgctttat
    38821 gccatatggc tgcaaaatag cgataaaaat acgttgattc gcgatctatt gaaaattaac
    38881 gtgctggatg aagtgtatta atctgaatgg ctggcaagga tgccggtaga aggatttact
    38941 tcagagaggg ttatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc
    39001 aggggcggag agactcgaac tcccaacacc cggttttgga gaccggtgct ctaccaattg
    39061 aactacgccc ctaattag
//
LOCUS       DABSYT020000035        38495 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.035, whole genome shotgun sequence.
ACCESSION   DABSYT020000035 DABSYT020000000
VERSION     DABSYT020000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.035"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..198
                     /locus_tag="ID725_RS11030"
                     /old_locus_tag="pgaptmp_002206"
     CDS             <1..198
                     /locus_tag="ID725_RS11030"
                     /old_locus_tag="pgaptmp_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850799.1"
                     /translation="NFLVNAGCQQVELAEQVDAIVSANLAADSDITKEKICEKYKSTI
                     EAFKKEQLALPVLVRRKNSET"
     gene            complement(261..815)
                     /gene="ytfJ"
                     /locus_tag="ID725_RS11035"
                     /old_locus_tag="pgaptmp_002207"
     CDS             complement(261..815)
                     /gene="ytfJ"
                     /locus_tag="ID725_RS11035"
                     /old_locus_tag="pgaptmp_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGV7850800.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1140..1346
                     /gene="ytfK"
                     /locus_tag="ID725_RS11040"
                     /old_locus_tag="pgaptmp_002208"
     CDS             1140..1346
                     /gene="ytfK"
                     /locus_tag="ID725_RS11040"
                     /old_locus_tag="pgaptmp_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGV7850801.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1425..2768)
                     /gene="paeA"
                     /locus_tag="ID725_RS11045"
                     /old_locus_tag="pgaptmp_002209"
     CDS             complement(1425..2768)
                     /gene="paeA"
                     /locus_tag="ID725_RS11045"
                     /old_locus_tag="pgaptmp_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGV7850802.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3091..3729)
                     /gene="msrA"
                     /locus_tag="ID725_RS11050"
                     /old_locus_tag="pgaptmp_002210"
     CDS             complement(3091..3729)
                     /gene="msrA"
                     /locus_tag="ID725_RS11050"
                     /old_locus_tag="pgaptmp_002210"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGV7850803.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            3935..5668
                     /gene="tamA"
                     /locus_tag="ID725_RS11055"
                     /old_locus_tag="pgaptmp_002211"
     CDS             3935..5668
                     /gene="tamA"
                     /locus_tag="ID725_RS11055"
                     /old_locus_tag="pgaptmp_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGV7850804.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            5665..9444
                     /gene="tamB"
                     /locus_tag="ID725_RS11060"
                     /old_locus_tag="pgaptmp_002212"
     CDS             5665..9444
                     /gene="tamB"
                     /locus_tag="ID725_RS11060"
                     /old_locus_tag="pgaptmp_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGV7850805.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            9447..9788
                     /gene="ytfP"
                     /locus_tag="ID725_RS11065"
                     /old_locus_tag="pgaptmp_002213"
     CDS             9447..9788
                     /gene="ytfP"
                     /locus_tag="ID725_RS11065"
                     /old_locus_tag="pgaptmp_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGV7850806.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10000..10251
                     /gene="chpS"
                     /locus_tag="ID725_RS11070"
                     /old_locus_tag="pgaptmp_002214"
     CDS             10000..10251
                     /gene="chpS"
                     /locus_tag="ID725_RS11070"
                     /old_locus_tag="pgaptmp_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HGV7850807.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10245..10595
                     /gene="chpB"
                     /locus_tag="ID725_RS11075"
                     /old_locus_tag="pgaptmp_002215"
     CDS             10245..10595
                     /gene="chpB"
                     /locus_tag="ID725_RS11075"
                     /old_locus_tag="pgaptmp_002215"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HGV7850808.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(10675..11205)
                     /gene="ppa"
                     /locus_tag="ID725_RS11080"
                     /old_locus_tag="pgaptmp_002216"
     CDS             complement(10675..11205)
                     /gene="ppa"
                     /locus_tag="ID725_RS11080"
                     /old_locus_tag="pgaptmp_002216"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGV7850809.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            11515..12471
                     /gene="ytfQ"
                     /locus_tag="ID725_RS11085"
                     /old_locus_tag="pgaptmp_002217"
     CDS             11515..12471
                     /gene="ytfQ"
                     /locus_tag="ID725_RS11085"
                     /old_locus_tag="pgaptmp_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGV7850810.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            12611..14113
                     /gene="ytfR"
                     /locus_tag="ID725_RS11090"
                     /old_locus_tag="pgaptmp_002218"
                     /pseudo
     CDS             12611..14113
                     /gene="ytfR"
                     /locus_tag="ID725_RS11090"
                     /old_locus_tag="pgaptmp_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
     gene            14127..15149
                     /gene="ytfT"
                     /locus_tag="ID725_RS11095"
                     /old_locus_tag="pgaptmp_002219"
     CDS             14127..15149
                     /gene="ytfT"
                     /locus_tag="ID725_RS11095"
                     /old_locus_tag="pgaptmp_002219"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGV7850811.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15136..16131
                     /gene="yjfF"
                     /locus_tag="ID725_RS11100"
                     /old_locus_tag="pgaptmp_002220"
     CDS             15136..16131
                     /gene="yjfF"
                     /locus_tag="ID725_RS11100"
                     /old_locus_tag="pgaptmp_002220"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGV7850812.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16164..17162)
                     /gene="fbp"
                     /locus_tag="ID725_RS11105"
                     /old_locus_tag="pgaptmp_002221"
     CDS             complement(16164..17162)
                     /gene="fbp"
                     /locus_tag="ID725_RS11105"
                     /old_locus_tag="pgaptmp_002221"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGV7850813.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            17338..18711
                     /gene="mpl"
                     /locus_tag="ID725_RS11110"
                     /old_locus_tag="pgaptmp_002222"
     CDS             17338..18711
                     /gene="mpl"
                     /locus_tag="ID725_RS11110"
                     /old_locus_tag="pgaptmp_002222"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGV7850814.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(18867..19418)
                     /gene="yjgA"
                     /locus_tag="ID725_RS11115"
                     /old_locus_tag="pgaptmp_002223"
     CDS             complement(18867..19418)
                     /gene="yjgA"
                     /locus_tag="ID725_RS11115"
                     /old_locus_tag="pgaptmp_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGV7850815.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            19512..20864
                     /gene="pmbA"
                     /locus_tag="ID725_RS11120"
                     /old_locus_tag="pgaptmp_002224"
     CDS             19512..20864
                     /gene="pmbA"
                     /locus_tag="ID725_RS11120"
                     /old_locus_tag="pgaptmp_002224"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGV7850816.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21048..21434
                     /gene="cybC"
                     /locus_tag="ID725_RS11125"
                     /old_locus_tag="pgaptmp_002225"
     CDS             21048..21434
                     /gene="cybC"
                     /locus_tag="ID725_RS11125"
                     /old_locus_tag="pgaptmp_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGV7850817.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(21479..21943)
                     /gene="nrdG"
                     /locus_tag="ID725_RS11130"
                     /old_locus_tag="pgaptmp_002226"
     CDS             complement(21479..21943)
                     /gene="nrdG"
                     /locus_tag="ID725_RS11130"
                     /old_locus_tag="pgaptmp_002226"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGV7850818.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22133..24271)
                     /gene="nrdD"
                     /locus_tag="ID725_RS11135"
                     /old_locus_tag="pgaptmp_002227"
     CDS             complement(22133..24271)
                     /gene="nrdD"
                     /locus_tag="ID725_RS11135"
                     /old_locus_tag="pgaptmp_002227"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGV7850819.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(24665..26320)
                     /gene="treC"
                     /locus_tag="ID725_RS11140"
                     /old_locus_tag="pgaptmp_002228"
     CDS             complement(24665..26320)
                     /gene="treC"
                     /locus_tag="ID725_RS11140"
                     /old_locus_tag="pgaptmp_002228"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGV7850820.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQLGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(26370..27791)
                     /gene="treB"
                     /locus_tag="ID725_RS11145"
                     /old_locus_tag="pgaptmp_002229"
     CDS             complement(26370..27791)
                     /gene="treB"
                     /locus_tag="ID725_RS11145"
                     /old_locus_tag="pgaptmp_002229"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGV7850821.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(27910..28857)
                     /gene="treR"
                     /locus_tag="ID725_RS11150"
                     /old_locus_tag="pgaptmp_002230"
     CDS             complement(27910..28857)
                     /gene="treR"
                     /locus_tag="ID725_RS11150"
                     /old_locus_tag="pgaptmp_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HGV7850822.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            29042..29095
                     /gene="mgtL"
                     /locus_tag="ID725_RS11155"
                     /old_locus_tag="pgaptmp_002231"
     CDS             29042..29095
                     /gene="mgtL"
                     /locus_tag="ID725_RS11155"
                     /old_locus_tag="pgaptmp_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGV7850823.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29236..31932
                     /gene="mgtA"
                     /locus_tag="ID725_RS11160"
                     /old_locus_tag="pgaptmp_002232"
     CDS             29236..31932
                     /gene="mgtA"
                     /locus_tag="ID725_RS11160"
                     /old_locus_tag="pgaptmp_002232"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGV7850824.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32138..32524)
                     /gene="ridA"
                     /locus_tag="ID725_RS11165"
                     /old_locus_tag="pgaptmp_002233"
     CDS             complement(32138..32524)
                     /gene="ridA"
                     /locus_tag="ID725_RS11165"
                     /old_locus_tag="pgaptmp_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGV7850825.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(32597..33058)
                     /gene="pyrI"
                     /locus_tag="ID725_RS11170"
                     /old_locus_tag="pgaptmp_002234"
     CDS             complement(32597..33058)
                     /gene="pyrI"
                     /locus_tag="ID725_RS11170"
                     /old_locus_tag="pgaptmp_002234"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGV7850826.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33071..34006)
                     /gene="pyrB"
                     /locus_tag="ID725_RS11175"
                     /old_locus_tag="pgaptmp_002235"
     CDS             complement(33071..34006)
                     /gene="pyrB"
                     /locus_tag="ID725_RS11175"
                     /old_locus_tag="pgaptmp_002235"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGV7850827.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34010..34144)
                     /gene="pyrL"
                     /locus_tag="ID725_RS11180"
                     /old_locus_tag="pgaptmp_002236"
     CDS             complement(34010..34144)
                     /gene="pyrL"
                     /locus_tag="ID725_RS11180"
                     /old_locus_tag="pgaptmp_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGV7850828.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(34425..34820)
                     /gene="yjgH"
                     /locus_tag="ID725_RS11185"
                     /old_locus_tag="pgaptmp_002237"
     CDS             complement(34425..34820)
                     /gene="yjgH"
                     /locus_tag="ID725_RS11185"
                     /old_locus_tag="pgaptmp_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGV7850829.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(34951..35664)
                     /gene="bdcA"
                     /locus_tag="ID725_RS11190"
                     /old_locus_tag="pgaptmp_002238"
     CDS             complement(34951..35664)
                     /gene="bdcA"
                     /locus_tag="ID725_RS11190"
                     /old_locus_tag="pgaptmp_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGV7850830.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            35735..36328
                     /gene="bdcR"
                     /locus_tag="ID725_RS11195"
                     /old_locus_tag="pgaptmp_002239"
     CDS             35735..36328
                     /gene="bdcR"
                     /locus_tag="ID725_RS11195"
                     /old_locus_tag="pgaptmp_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGV7850831.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            36473..36925
                     /gene="tabA"
                     /locus_tag="ID725_RS11200"
                     /old_locus_tag="pgaptmp_002240"
     CDS             36473..36925
                     /gene="tabA"
                     /locus_tag="ID725_RS11200"
                     /old_locus_tag="pgaptmp_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGV7850832.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37048..>38495
                     /gene="yjgL"
                     /locus_tag="ID725_RS11205"
                     /old_locus_tag="pgaptmp_002241"
     CDS             37048..>38495
                     /gene="yjgL"
                     /locus_tag="ID725_RS11205"
                     /old_locus_tag="pgaptmp_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HGV7850833.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAGGQD
                     NTRNILNPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSPKVIIEISLSTITTMGTRQGHTAIIFPQPDGSTNRYERKSFERKDE
                     SSLHLITNKVLACYQREANKEIARLLNNHQKLNNLQKLNNLQKLNNIQKLNNIQELNN
                     SQELNNSQELNNSQDLKNSQVSCKGSVDFTITDLLEKSLNNALLAIRNEHLLLMPHVC
                     SESISYLLGENGILEEIDKLYELNDHGIDNDKEGNNEINDIMINLSHILIESLDDAKV
                     NLTPVIHSMLMTFLELPYNNDVKILEWCFNKSMQYFDDSAKIEHACSVINHINFRRDQ
                     SKVAETLFFNLDKEPYKNSPELQELIWKKLVVYVNDFNLSNREKTYLIQRIFNNVESL
                     FNKVPVSILVNDIFMNDFFMKNTEMINWYFPR"
BASE COUNT         9411 a         9465 c        10242 g         9377 t
ORIGIN      
        1 aattttctgg ttaatgcggg ttgccaacaa gtcgaattag ccgaacaagt ggatgcgata
       61 gtaagtgcga atctggccgc agatagtgat attaccaaag aaaagatttg cgagaaatat
      121 aaatcgacca ttgaggcgtt taaaaaagag caactagcgt taccagtatt agttcggcgt
      181 aagaactcag agacttaatt cccctatccc atagataacg ataggggaaa attatttttg
      241 cagaatttat gttattgcca ttatttgagc aacttttgca gcaggtccat cacttgctgc
      301 acctcttcct gagtgagcgc cccgtctttg gcccattgca cgcgaccgtc tttatccagc
      361 accactacag cggaactttc ttcatccagc tgccaggcac cgcgcgcgac gccattgcta
      421 tcgacaataa actgcgacca gggataaagc tttttattac tctccagact gctgcgcaca
      481 aacatgccgg aacccggaat cgcgtcgtcg gtgttaacaa tggtggtggt ctggtaacga
      541 tcgtgcggta acttcgctga tttaatcgct tcaatcagcg tcgcgttttt ctcttttgca
      601 gaggtgcgac cagcaatatg ttgcagtact cgcacttttc ccactaactg cgcgctgttc
      661 caggttttgt agctaaactg atctttatca agcaccaact cgccccgatc ggtaatgcca
      721 atcggcggca ctcgctgacc ggtttcgaac tgatgtgcgg aagccatcat cggcaacagc
      781 aggcaggtga gtgccagaat cttgcgtagg gtcatggtgt ttccttctta tgatatgcag
      841 gtgatccgac cacttgggtc tgagttttaa tcatatgtgc tatttatcga atttcccgca
      901 agtgtgatgc cagtttgcgg tcaagcgcac aaatcatatg aaaaatgaat gcttatactg
      961 aagaccgcgc ttcggtaaaa agataattct gaataattgt aacctttagg taaaaaaagt
     1021 tatacgcggt ggaaacattg cccggatagt ctatagtcac taagcattaa aatttgcgcc
     1081 tcataatagt tgggccgatt gtggcaccgc acaggcgtaa tactcagcag gagataacaa
     1141 tgaaaatttt ccaacggtac aacccacttc aggtggcgaa gtacgtaaag atcctgttcc
     1201 gtggacgatt atacatcaag gacgttggcg cttttgaatt cgataagggt aagattctta
     1261 tcccaaaagt gaaggataag ctgcatttgt cagtgatgtc cgaagttaac cgtcaggtta
     1321 tgcgtctgca aacagagatg gcttaaccaa agtgctatgc agtaaaaagt gctatgcagt
     1381 aataagacgg ctcctgattc aggagccgtt gatgtttatg ggggttacgc gacgctttct
     1441 tctttatctt tagcgtcagg cagttttggc gaaagggctg tggccttgct gtcgatccgg
     1501 gtcaccagca gctggtcgat gcggtagtta tcgatatcca ccacttcaaa tttgtagccg
     1561 gcgaatttca ccgaatcggt gcgtttcggg atcttacgca gcataaacat cataaagccg
     1621 ccgatggttt cgtagttgcc cgactgcggg aactcgtcaa tatccagcac gcgcatgacg
     1681 tcgtcaatcg gagtgccgcc gtcaatcagc catgagttct catcacgggc gacaatctgc
     1741 tcttccagtc cctgaccgac cagatcgccc atcagcgtgg tcatcacgtc gttgagggtg
     1801 atgatcccca ctaccagcgc gtactcgttc atgatcaccg cgaagtcttc tcctgcggtt
     1861 ttaaaacttt ccaacgcctc tgaaagggtt aacgtatccg gcacaatcag cgtgttgcga
     1921 atttgtacgc cgctgttcag tgccaggctt tggttagcca gcacgcggtt cagcaggtct
     1981 ttagaatcga catagccgat gatgtggtca atatcttcat tacagacgag gaacttagag
     2041 tgcggatgtt ccgccacctt atttttcaga ctttgctcat cttcgtggag atcaaaccaa
     2101 atcacgtttt cacgcggtgt cattgaagac ggaacggtac gggattccag ctcaaagacg
     2161 ttttcaatca gctcgtgttc ctgtttacgt aacacgcccg ccagcgcacc ggcttccact
     2221 accgcgtaga tgtcatcaga agtgatgtca tctttacgta ccattggcag tttgaaaata
     2281 cggaagatta tgttcgccag gccgttgaag aaccacacca gcggggtgca aacgtacagg
     2341 cagaagcgca tcgggttgat gatacgcaaa gccacagctt ctggcgcaat cataccgatg
     2401 cgtttcgggg ttaaatccgc aaacaggata aacatgccag tcactaacga gaaagagaga
     2461 ataaagctca gttgctcaga gagctctgcc gacatatagc gggagaacag gctgtgaaaa
     2521 gctggagaaa atgccgcatc accgacgata ccgccgagaa tcgccactgc gttcagaccg
     2581 atttggacca cggtaaagaa catgccggga ttttcctgca tattcagaac gcgttgggca
     2641 tttatattgc cttcatcagc cagcagttta agtttgattt tgcgtgaggc ggcaagtgag
     2701 atctcagaca tcgagaagaa cgcacttaca gcgatcaagc agagtatgac taaaatactg
     2761 tttaacatat cttatccgac ctttcaggcc agatcctcgg aaggggaagt tgattaattt
     2821 gtgtgaaata cacattgaaa gccgattcga agaaagtgaa ccggcatttt cagtggctag
     2881 tatagcgtaa ggtactgtaa agccgccaga gggttaaaat tcagacagct gaaaaatgca
     2941 aaactgcctg atgcgctatg ccgatcaggc ctacgcagtc cttgcaatat attgaatttg
     3001 gacgattttg taggcctggc aaggcgttta cgccgcatcc ggcataaaca aaacgcactt
     3061 tatcaacaat ctacatttgt acccgtaacg ctatgcttcc ggtggcagac agacgccaat
     3121 tccgccaatt ccacagtaac cgtacgggtt tttatgcaga tattgctggt ggtcatcttc
     3181 ggcataataa aacggtgtgg cgttagcgat ttccgtggtg atgtgacgat cgtcatcggc
     3241 ggcaagcatc gccgcctgaa aacgttccag actggcgcga gctgcggcat cctgttctgg
     3301 ggtcagcgga taaatcgctg aacgatactg cgtgccgtgg tcattgccct gacgcattcc
     3361 ctgggcggga tcgtgattct cccaaaatac ctgtagcaac tgctcatagc tgatgacgga
     3421 aggatcgtaa acaatgcgta ccgcttcggc atgaccagta tcaccggagc acacttcccg
     3481 ataagtcgga tttggcgtat agccgccggt ataacctgcg gcggtgctgt aaacgccgtg
     3541 taactgccag aacagacgct ccacacccca gaaacaaccc atcgcaaaaa tggcaatctc
     3601 cattccgtca ggtacattgg tcattgagtg accgttaacc gcatgcagcg tggctacggg
     3661 catcggggtg ttacgtccag gcagggcatc ggcgggggaa accagatgct ttttatcaaa
     3721 taaactcatg gtgtcgctct cccgaaaatc ggtcattggg gttaaggttg taacaagagg
     3781 cgtatttgca cacaataacc accgtgaata ggtctaaagt aaaacataag aaatatttgg
     3841 ggtttagtct gctttttaat ccatattact ggatttttgt taagccgttt aacggcgttc
     3901 caggggcagg aaaaaaggat attcaggaga aaatgtgcgc tatatccgac agttatgctg
     3961 tgtaagctta ctctgcttaa gcggatctgc cgtcgccgcg aacgtccgtc tacaggtcga
     4021 ggggttatcg ggacagctgg aaaagaacgt tcgtgcgcag ctttctacga ttgaaagtga
     4081 tgaagtgacg ccagaccgtc gctttcgcgc acgcgtcgat gatgctatcc gcgaaggtct
     4141 gaaagcgctg ggttattacc agccgaccat tgaatttgat ctccgtccac cgccaaagaa
     4201 agggcggcag gtattgatcg ccaaagtcac gccaggcgtg ccggtgttaa ttggcggcac
     4261 cgatgtggta ttgcgcgggg gcgcgcggac cgataaagac tatttgaaat tgctcgatac
     4321 tcgcccggct attggcacgg tactgaacca gggcgattat gaaaatttca aaaagtcctt
     4381 aaccagcatt gcgttgcgta aaggttattt cgatagcgaa tttaccaaag cgcagctggg
     4441 cattgcgctc ggcctgcata aagccttctg ggatattgat tataacagtg gcgaacgtta
     4501 ccgctttggg catgtgacct ttgaaggatc acaaatccgc gatgaatacc tgcaaaatct
     4561 ggtgccgttt aaagagggcg atgagtacga atcgaaagat ctggcagaac tgaaccgccg
     4621 actttctgct accggctggt ttaactcggt ggtggtggct ccacaatttg ataaagcgcg
     4681 cgaaacgaaa gtattaccat tgacgggcgt ggtttcgccg cgaacagaaa acactatcga
     4741 aaccggggtc ggttactcta cggacgtggg accgcgcgtg aaagcgacgt ggaaaaaacc
     4801 gtggatgaac tcatacggtc acagtctgac caccagtacc agtatttccg cgccggaaca
     4861 gaccctcgac ttcagctata aaatgccgct gctgaagaat ccactggaac aatattattt
     4921 ggtgcagggc ggttttaagc gcactgacct gaacgatacc gagtctgact ccactacgct
     4981 ggtggcttct cgctactggg atctctccag cggctggcag cgtgccatta acctgcgctg
     5041 gagtctcgac cactttactc agggtgaaat taccaacacc acgatgctgt tttatcctgg
     5101 ggtgatgatt agccgcacgc gttctcgtgg tggcctgatg ccaacctggg gcgactcgca
     5161 acgctactct atcgactact ccaacacggc ctggggttca gatgtcgatt tctccgtttt
     5221 ccaggcgcag aacgtctgga tccgcacact gtacgatcgc catcgttttg tgacacgcgg
     5281 cacgctgggc tggattgaaa ccggtgattt cgacaaagta ccgccggatc tgcgtttctt
     5341 cgccgggggc gatcgcagta ttcgcggcta caaatacaaa tctatcgccc cgaaatacgc
     5401 taacggtgac ctgaaagggg cctcgaagtt gataaccgga tcgctggaat accagtacaa
     5461 cgtgaccgga aaatggtggg gcgcggtgtt tgtcgatagt ggcgaagcgg taagcgatat
     5521 tcgccgcagc gactttaaaa ccggtaccgg ggtcggcgta cgctgggaat cgccggtcgg
     5581 gccaatcaaa ctcgattttg ccgtaccggt cgcggataaa gacgaacacg ggttacagtt
     5641 ttacatcggt ctggggccag aattatgagt ttatggaaaa aaatcagcct cggcgtggtt
     5701 atcgttatct tactgttgct gggatcggtg gcgtttctgg tgggcaccac cagcggcctg
     5761 catctggtat ttaaagcggc ggatcgctgg gtgccaggac tggatattgg caaggtcacc
     5821 ggcggctggc gcgatctcac cttgtctgac gttcgttatg agcagccagg cgtggcggta
     5881 aaagcgggta atctgcatct ggcggtcggt cttgagtgcc tgtggaacag cagtgtttgt
     5941 attaatgact tggcgctgaa agacattcag gtcaacatcg acagtaaaaa aatgcctcct
     6001 tctgaacagg ttgaagaaga ggaagatagc ggtccgctgg atctctccac gccgtatccc
     6061 atcaccctga cacgggtggc actggacaac gtcaacatca agattgatga caccacggta
     6121 tcggtgatgg acttcacctc cggcctgaac tggcaggaga aaaccctgac cctgaaaccg
     6181 acgtcgctga aaggcctgct gattgctctg ccgaaagtgg cggaagtggc gcaggaagaa
     6241 gtggtcgaac cgaaaattga aaatccgcag ccggaggaaa agcctctcgg cgaaacactc
     6301 aaagatctct tttctcgccc ggtattgccg gaaatgaccg acgtgcatct gccactcaac
     6361 ctgaacattg aagagtttaa gggagagcaa ctgcgcgtga cgggcgatac ggacattacc
     6421 gtgcgcacca tgctgctgaa agtgagcagc attgacggca atactaaact ggacgccctg
     6481 gatatcgatt ccaatcaagg gatcctcaac gccagcggca cggcgcagct gtcagacaac
     6541 tggccggtgg atatcaccct caacagcaca ctgaacgtgg agccgttgaa aggtgaaaag
     6601 gtgaagctga aagtgggcgg cgcgctgcgc gaacagctgg agattggcgt taatctttcc
     6661 ggtccggtgg atatggattt acgcgcccag acgcgactgg cggaagccgg attgccgctc
     6721 aacgtggaag tgaacagcaa acagatttac tggccgttca ctggtgagaa gcagtatcag
     6781 gcggatgatc tgaaactgaa acttaccggc aaaatgaccg attacacgct ctctatgcgt
     6841 acggcagtga agggactgga gatcccgcca gccaccatta cccttgatgc caaaggtaat
     6901 gaacagcagg tcaatctcga caaactcacc gtcgcggcgc tggaagggaa aactgaactc
     6961 aaggcgttgc ttgactggca gcaggccatt agttggcgcg gtgagctaac gcttaacggc
     7021 attaacaccg ccaaagagat cccagagtgg ccgtcgaaac tcaatggctt gattaaaacc
     7081 cgcggtagcc tgtacggcgg cacctggcag atggaggtgc cagaactgaa gcttaccggt
     7141 aacgtcaaac agaacaaagt gaacgttgac ggcacgctga aaggcaacag ttatatgcag
     7201 tggatgatcc cagggcttca tctggaactg gggccaaaca gtgccgaagt gaaaggcgag
     7261 ctgggggtaa aagatctcaa tcttgatgcc accatcaacg cgccggggct ggataacgcg
     7321 ctgccggggc ttggcggtac agcgaaaggg ctggtgaaag ttcgcggcac ggtggaagcg
     7381 ccacaactac tggcggacat caccgcgcgc ggcctgcgct ggcaggagct aaccgtggcg
     7441 caggttcgcg tggaaggcga tatcaaatcc accgatcaga ttgcgggtaa actcgacgtg
     7501 cgcgttgagc aaatttcaca gccggatgta aatatcaacc tcgtcaccct gaatgccaaa
     7561 ggcagcgaaa aacagcacga gttacagttg cggattcagg gcgagccggt ttccgggcag
     7621 cttaatctgg caggaagttt tgatcgcaaa gaagaacgct ggaagggaac tcttagcaat
     7681 acccgcttcc agacgctggt cggcccgtgg tcgctgaccc gcgatattgc gctggattac
     7741 cgcaataagg agcaaaaaat cagcatcggg ccacactgct ggcttaaccc gaatgcggaa
     7801 ctgtgcgtgc cgcaaactat cgatgcgggg gccgaagggc gtgcggtggt gaatctcaac
     7861 cgcttcgacc ttgccatgct gaaaccgttt atgccagaaa ccactcaggc cagcggtatc
     7921 ttcacgggta aagcggacgt tgcctgggac accaccgaag aggggctgcc gcagggcagt
     7981 atcacccttt cggggcgtaa cgtgcaggta acgcaaaccg tcaacgatgc ggcgctgccc
     8041 gtggcgtttc agacactgaa tctgacggcg gaattgcgta acaaccgtgc cgaattgggc
     8101 tggaccatcc gcctgaccaa taacggccag tttgatggac aggtgcaggt gaccgatccg
     8161 caaggccgcc gtaatcttgg tggcaacgtc aatatccgta acttcaacct tgcgatgata
     8221 aaccccatct ttacccgtgg ggaaaaagca gcggggatgg tgagtgccaa cttgcgtctg
     8281 ggtggtgatg tgcaaagccc gcagttgttt ggtcagcttc aggttacggg tgtggatatc
     8341 gatggcaact ttatgccgtt tgatatgcaa ccgagccagc ttgcggtcaa ctttaacggt
     8401 atgcgctcga cgcttgccgg tacagtacga acccagcagg gtgaaatcta cctgaacggt
     8461 gatgccgact ggagccaaat tgaaaactgg cgggcgcggg taacggcgaa aggcagtaaa
     8521 gtgcggatca ccgtgccgcc gatggtacga atggatgtat cgccagatgt tgtattcgag
     8581 gctacaccaa acctgtttac cctcgatggt cgcgtggatg tcccttgggc gcgcatcgtg
     8641 gtgcacgatc tgccggaaag cgcagtaggc gtctccagcg atgtggtgat gcttaacgat
     8701 aacctgcaac cggaagagcc gaaaacggcg tcgattccga ttaacagtaa cctgattgtc
     8761 cacgttggca acaatgtgcg cattgacgcc tttggcctga aagcgcggct gacgggcgat
     8821 cttaacgtcg ttcaggacaa acaagggctg ggcctgaacg ggcagatcaa catccctgaa
     8881 gggcgcttcc atgcctatgg tcaggatctg attgtgcgta aaggtgagtt actgttctct
     8941 ggcccgccgg atcaaccgta tcttaatatt gaagctattc gtaacccgga tgctacagaa
     9001 gacgacgtaa tcgccggagt tcgcgtcact ggtctggcgg acgaaccgaa agcggagatc
     9061 ttctctgacc cggcgatgtc gcaacaagct gccttgtctt atttgctacg tggacaaggg
     9121 ctggagagcg atcagagcga cagtgcggca atgacctcga tgctgattgg tctgggggtt
     9181 gcgcaaagtg gccagattgt aggtaaaatc ggcgagacgt ttggcgtaag caatttagcg
     9241 ctcgacaccc agggagtagg cgactcctcc caggtagtgg tcagcggcta tgtattgcca
     9301 ggtctgcaag tgaaatacgg cgtgggtata tttgactcta tagcaacact cacgttacgt
     9361 tatcgcctga tgcctaagct atatctggaa gccgtgtctg gtgtagacca ggcactggat
     9421 ttgctctatc agttcgagtt ttagcaatgc gaatatttgt ctacggcagt ttacgccaca
     9481 aacaaggcaa cagtcactgg atgaccaatg cccagttact gggcgatttc agtatcgata
     9541 actaccagtt gtatagcctg ggccactatc caggcgcagt tccggggaac ggaacggtac
     9601 acggtgaagt ttatcgtatt gacaacgcca cgctggccga acttgatgcc ttgcgcacca
     9661 ggggcggtga atacgcgcgc cagttgattc agacgccgta cgggagtgca tggatgtacg
     9721 tttatcaacg acccgtcgat ggattaaagc taattgaaag cggcgactgg ttagacaggg
     9781 ataagtaacc ctatgcatac gccaccttcg ggtggcgttg ttttttgcga gacgactcgc
     9841 attctgtttt gtaattccct caccttttgc ttttctctcc gagccgcttt ccatatctat
     9901 taacgcataa aaaactctgc tggcattcac aaatgcgcag gggtaaaacg tttcctgtag
     9961 caccgtgagt tatactttgt ataacttaag gaggtgcaga tgcgtattac cataaaaaga
    10021 tgggggaaca gtgcaggtat ggtcattccc aatatcgtaa tgaaagaact taacttacag
    10081 ccggggcaga gcgtggaagt gcaggtgagc aacaaccaac tgattctgac acccatctcc
    10141 aggcgctact cgcttgatga actgctggca cagtgtgaca tgaacgccgc ggaacttagc
    10201 gagcaggatg tctggggtaa atccacccct gcgggtgacg aaatatggta aagaaaagtg
    10261 aatttgaacg gggagacatt gtgctggttg gctttgatcc agcaagcggc catgaacagc
    10321 aaggtgctgg tcgacctgcg cttgtgctct ccgttcaagc ctttaatcaa ctgggaatga
    10381 cgctggtggc ccccattacg cagggtggaa attttgcccg ttatgccgga tttagcgttc
    10441 ctttacattg cgaagaaggc gatgtgcacg gcgtggtgct ggttaatcag gtgcggatga
    10501 tggatctacg cgcccggctg gcaaagcgta ttggtctggc tgcggatgag gtggtggaag
    10561 aggcgttatt acgcttgcag gcggtggtgg aataaggtgt gtttatttat cgcgggcata
    10621 aaaaaaccct tactaaccga agcccggcgt tcagggttat tacgccagaa gaacttattt
    10681 attctttgcg cgctcgaagg aggcaacgat ttcagcttta gcggcttctg cgttttccca
    10741 accttcaact ttcacccact tgcctttttc gaggtctttg tagtgctcga agaagtgagc
    10801 gatttgcgct ttcagcagtt caggcagatc gttaacgtct ttaatgtgat cgtattcttt
    10861 gctcagcttg ctgtgcggaa ccgcaaccag tttcgcatct tcaccggctt cgtcggtcat
    10921 tttcagaacg ccaaccggac ggcaacggat cacagaaccc ggctgcagcg ggtacggagt
    10981 tgggaccagt acgtcaactg ggtcaccgtc cagagacagg gtgtggttga tgtaaccgta
    11041 gttgcacgga tagaacatcg cggtggacat gaagcggtca acgaacagtg cgccgctctc
    11101 tttgtcgatt tcgtatttga tcggatctgc gttagccggg atctcaataa caacgtagat
    11161 gtcttccggc agatctttac ccgcagggac gttgagtaag ctcatgtctg tttcctttaa
    11221 aaatatgtgg caaacaagtg ccgagtatta tagccaactc gcgccgaatg tcttcgcttg
    11281 ttttcgtctt cattttcctt tttcgccagt tttaagacgg tatgcagagc aggaaaatcc
    11341 ataaccgtag ccgcattttt catagtgaga tgaaagcgat tacaaacttg tgattaacgt
    11401 tttatttact tttttgaagt gtgatgtaac gcaatccgtt acataacgaa ttgtctatag
    11461 ttttttcgcg aacatctttt caccaataat aattactccg acgaggacaa ccctatgtgg
    11521 aaacgcttac ttgtagtctc tgcagtctcg gcagccatgt cgtctatggc gttggccgct
    11581 ccattaaccg taggattttc gcaggtcgga tcggaatccg gctggcgcgc cgcagaaacc
    11641 aatgtggcga aaagtgaggc cgaaaaacgc ggaattacgc tgaaaattgc cgatggtcag
    11701 caaaagcagg aaaaccagat taaagcggta cgttccttcg ttgcacaagg ggtggatgcg
    11761 atctttatcg ctccggtggt cgcgacaggt tgggaaccgg tattaaaaga ggcgaaagat
    11821 gccgaaatcc cggtcttctt gctcgaccgt tccatcgatg tgaaagacaa atctctctat
    11881 atgaccaccg ttactgccga caacatcctc gaaggtaagt tgatcggtga ctggctggta
    11941 aaagaagtga atggcaaacc atgcaacgtg gtggagctgc agggcaccgt tggggccagc
    12001 gtcgccattg accgtaagaa aggctttgcc gaagccatta agaatgcgcc aaatatcaaa
    12061 atcatccgct cgcagtcagg tgacttcacc cgcagtaaag gcaaagaagt catggagagc
    12121 tttatcaaag cggaaaacaa cggcaaaaac atctgcatgg tttacgccca taacgacgac
    12181 atggtgattg gtgcaattca ggcaattaaa gaagcgggcc tgaaaccggg caaagatatc
    12241 ctcacgggtt ccattgacgg tgtaccggac atctacaaag cgatgatgga tggcgaagcg
    12301 aacgccagtg ttgaactgac gccgaacatg gcgggacccg ctttcgatgc gctggagaaa
    12361 tacaaaaaag acggcaccat gcctgaaaag ctgacgttaa ccaaatccac cctttacctg
    12421 cctgataccg caaaagaaga gttagagaag aagaaaaata tggggtattg agggttgcta
    12481 tgcctgatgc cgattcgtag gccggataag gcgctcgcgc cgcatccggc gatggtgcac
    12541 tgaagcctga tgcgacgctt accgcgtctt atcatgccta ctgggagcac gctttacacc
    12601 gggggaaacc atgacgaccg accaacacca ggagatcctc cgcaccgaag gattaagtaa
    12661 atttttcccc ggcgtcaaag cgttagacaa cgttgatttc agcctgcgcc gtggcgaaat
    12721 catggcgctg ctcggtgaaa acggggcggg aaaatcaacg ctaatcaaag cattaactgg
    12781 tgtataccac gctgatcgcg gcaccatctg gctggaaggc caggctatct caccgaaaaa
    12841 taccgcccac gcgcaacaac tcggcattgg caccgtctat caggaagtca acctgctacc
    12901 caatatgtcg gtcgctgata atctatttat aggccgcgaa cccaaacgct tcggccttct
    12961 acgccgcaaa gagatggaaa agcgcgccac cgaactgatg gcatcttacg gtttctccct
    13021 cgacgtgcgc gaaccgctca accgcttttc agtcgcgatg cagcaaatcg tcgctatttg
    13081 ccgggctatc gatctctctg ccaaagtgct gatcctcgat gaacccaccg ccagtctcga
    13141 cacccaggaa gtggagttac tgtttgacct gatgcgtcag ttgcgcgatc gcggcgtcag
    13201 cctgatcttt gtcactcact ttctcgatca ggtctatcag gtcagcgatc ggatcaccgt
    13261 cttacgcaac ggcagtttcg taggctgtcg ggaaacgtgc tagctaccgc agatcgaact
    13321 ggtaaaaatg atgctggggc gcgagctgga tacccacgcg ctacagcgtg ccgggcgaac
    13381 attgttgagc gacaaacccg ttgccgcgtt caaaaattac ggcaaaaaag gaacgatcgc
    13441 accgtttgat ctcgaagtac gccccggcga gatcgtcggt ctggctggat tgctgggatc
    13501 aggacgtacc gaaaccgccg aagtgatctt cggtatcaaa cctgctgaca gcggcacggc
    13561 gttgatcaaa ggcaaaccgc aaaacctgcg atcgccacat caggcttcgg tacttggcat
    13621 tggcttctgc ccggaagaca ggaaaaccga tggcatcatc gctgccgcct cggtgcggga
    13681 aaatatcatc ctcgctctct aggcccagcg cggctggcta cgtcccattt cccgcaaaga
    13741 acagcaagag attgccgaac gctttatccg ccagcttggc attcgcacac cttcaactga
    13801 acaaccgatt gaatttctct ccggcggcaa tcagcaaaaa gtgttgcttt cacgttggct
    13861 actgacccga ccgcaatttc tgatcctcga tgagccaacc cgcggcattg atgttggtgc
    13921 ccacgccgag atcatccgcc tgattgaaac gctatgcgcc gatggtctgg cgctgctggt
    13981 gatctcctcc gaactggaag aactggtggg ctatgccgac cgggtgatca tcatgcgcga
    14041 tcgcaaacag gtggcggaga tcccgctggc agagctttcc gttccggcga tcatgaatgc
    14101 cattgcggcg taaggagaac agtgtgatgc ctcaatctct cccggacact acgccgccaa
    14161 aaaggcgctt tcgctggcca acgggaatgc cgcagctggc ggcactgttg ctggtgctgc
    14221 tggtcgatag cctggtggcc ccgcatttct ggcaggtggt gcttcaggat gggcgtttgt
    14281 tcggtagccc tatcgacatt cttaatcgcg ccgcccccgt tgcgctactg gcgattggta
    14341 tgacgctggt gatcgcaaca ggtgggattg atctctccgt aggggcggtg atggctatcg
    14401 ccggagccac aacggctgcg atgacggtcg cgggattcag cctgccgatt gttttgttaa
    14461 gcgccctggg caccggcatc ctggcgggat tgtggaacgg catactggta gcgatcctca
    14521 aaattcagcc gtttgtcgcc actctgatcc tgatggtcgc cgggcgcggc gtggcgcaac
    14581 tgatcacctc cggacagatc atcacgttta actcgccgga tctctcatgg ttcggcagtg
    14641 gatcgctgtt gttcctgcca acgccggtca ttatcgcggt gctgacgctt atcctgttct
    14701 ggctgttgac ccgcaaaacg gcgctgggga tgtttatcga agccgttggt atcaacattc
    14761 gggcggcaaa aaatgccggg gtaaacacgc ggatcatcgt catgcttacc tacgtgttga
    14821 gcgggctgtg tgcggcgatt gcgggcatta tcgtggcggc ggatattcgc ggtgccgatg
    14881 ccaacaacgc cggattatgg ctggagctgg acgccattct cgcggtggtg attggcggtg
    14941 gttcgctgat gggcggacgt tttaacctac tgctttcggt ggtgggggcg ctgattattc
    15001 aggggatgaa caccggaatt ttgctttcgg gctttccgcc agagatgaac caggtggtga
    15061 aagcggtggt ggtgctttgc gtgctgattg tccagtcgca acgctttatc agtctgatta
    15121 aaggagtgcg tagccgtgat aaaacgtaat ttgccactga tgatcaccat cggcgtcttt
    15181 gtgctgggtt atctctactg cctgacccag tttcccggtt ttgcttccac aagagtgatc
    15241 tgcaatatcc tgaccgataa tgcctttctt gggataatcg ccgttggcat gacctttgtg
    15301 atcctctccg gtgggatcga tctctccgtt ggttcggtga tcgcctttac cggcgtgttt
    15361 ctggcaaaag tgattggcga tttcggcctc tcgccgttgc tggcatttcc gctggtgctg
    15421 gtaatgggct gtgcctttgg cgcatttatg gggcttttga tcgacgccct gaagatcccg
    15481 gcatttatca ttactttggc aggaatgttc tttttgcgcg gcgtcagcta cctcgtttcg
    15541 gaagagtcga tcccgataaa ccatcctgtt tatgacacgc tctcaagcct tgcatggaaa
    15601 attcctggcg gcggtcgctt aagtgcgatg gggctgctga tgctggcggt ggtggttatc
    15661 ggcatattcc tcgcgcatcg tacccgcttt ggtaatcagg tgtacgccat tggcggcaat
    15721 gcgacgtcgg caaacctgat ggggatttcc actcgcagca caactattcg catttatatg
    15781 ctctctaccg gactggcgac actggcgggg attgtcttct cgatttatac ccaggccgga
    15841 tacgcgctgg cgggcgtagg tgtggaactg gacgctatcg cctcagtggt gattggcggt
    15901 acgcttttga gcggtggcgt tggaacggta ttagggacgc tttttggcgt ggcgattcag
    15961 ggactgattc agacttacat aaactttgat ggtacgctga gctcatggtg gacgaaaatc
    16021 gccatcggta ttttgttgtt tatttttata gcattacagc gtggattaac ggtgctgtgg
    16081 gagaatcgtc agagttcgcc tgtgacaaga gtcaacattg cgcagcaata aaatcgcctc
    16141 tccgtgtgga gaggcgcagg agattacgcg tccgggaact cacagataaa gcgttcgaca
    16201 tcttcaacca tatggtcgtt gccgacaaag aatgaacggc gctggtgcag ggtttccggg
    16261 ataatatcca gaatacgctc tttgccatcg ctcgctttac cgcccgcttg ttccgccagg
    16321 aatgccatcg ggttgcactc atacagcaaa cgcagtttgc cgtccgggtg gctggcggtg
    16381 cttgggtaga gataaatacc gcctttcagt aggttacggt ggaaatccgc aaccagtgag
    16441 ccgatataac gtgaggtata cgggcggttg gtggatttat cttcttcctg gcagaattta
    16501 atgtacttct tcaccccgtt cggaaactta atgtagtttc cttcgttgat ggagtaggtt
    16561 ttgcctttct ccgggaagcg catccgttcc tggcacaggc agaaaacgcc gagcgaagga
    16621 tcgtaagtaa aagcgtgaac accgcatccg gtactgtaaa ccagcatggt agaggagccg
    16681 tataccacgt aacctgccgc aacctgtttg ttaccaggct ggaggaaatc ttcttccgtt
    16741 accggcgtgc cgacgggcgt aactcggcgg tagatggaga aaatggtacc gacagagacg
    16801 ttaacatcga tgttggacga gccatccagt gggtccatca gcaccacgta ttttgcatgt
    16861 tcacagcctt caaagacaac aatctcatct tcttcttcag aggcaatgcc tgcaacgata
    16921 tcgcgtgctt tcagtgcggc tttcagtttt tcattagcga acaagtcgag tttttgctga
    16981 acctcgccct gcacgttctc agcaccgctg gcacccagga tatcaaccag tcctgctttg
    17041 ttgatatcgc gatggataat cttggcgccc agttttattg ccgacagcaa agcagtgagc
    17101 tcaccggtag catgagaaaa ctcgtgctgc ttttcgacaa taaattcacc taacgttttc
    17161 ataaaacttt ccctgcaatg tttatggagt aaagcgaccg caacaatctt aacaaataat
    17221 ctcaatgttg cgctcaggtg aatcgcgcca gcaaattacg gattatcctg aaatgcgttt
    17281 ctcacttgcc cgacatatgc gtaaaatgag cggcagatta aaaaaggata gtgacgcatg
    17341 cgcattcata ttttaggaat ttgtggcacg tttatgggcg gtctggcgat gctggcgcgc
    17401 cagttaggcc atgaagtaac gggttcggac gccaatgtgt atccgccgat gagcacctta
    17461 cttgagaagc aaggcattga gctgattcag ggttacgatg ccagccagct cgacccgcag
    17521 ccggatctgg tgattattgg caacgccatg acccgtggaa atccgtgtgt ggaagcggta
    17581 ctggaaaaaa acatccctta tatgtcaggt ccacagtggc tgcacgattt tgtgctgcgc
    17641 gaccgctggg tgctggccgt tgccggtaca cacggcaaaa ccaccaccgc gggaatggcg
    17701 acttggattc tggaacagtg tggttacaaa ccgggctttg taatcggcgg tgtgccgggg
    17761 aactttgagg tttcggcgcg tctgggcgaa agcgacttct ttgttatcga agcggatgag
    17821 tacgactgcg ccttcttcga caaacgctct aaatttgtcc attactgccc gcgtacgctg
    17881 atcctcaaca accttgagtt cgatcacgcc gatatctttg acgacctgaa agcgatccag
    17941 aaacagttcc accatctggt gcgtatcgtt ccggggcagg gtcgtattat ctggccggaa
    18001 aatgacatca acctgaaaca gaccatggcg atgggctgct ggagcgagca ggagctggtg
    18061 ggtgagcagg gtcactggca ggcgaaaaag ctgaccaccg atgcttccga atgggaagtt
    18121 ttgctggatg gcgaaaaagt gggcgaagtg aaatggtcgc tggtaggcga acataatatg
    18181 cacaatggcc tgatggcgat tgcagcggct cgccatgttg gtgtagcgcc ggcagatgcc
    18241 gctaacgcgc tgggttcgtt tattaacgct cgccgccgtc tggagttgcg tggtgaagcg
    18301 aatggcgtca cggtatatga cgattttgcc catcatccga cggcgattct ggcaacgctg
    18361 gcggcgctgc gtggcaaagt tggtggtacg gcgcgcatta ttgctgtgct ggagccgcgc
    18421 tcgaatacca tgaaaatggg gatctgcaaa gacgatctgg caccttcatt aggtcgtgcc
    18481 gatgaagtct tcctgctgca accggcgcat attccgtggc aggtggcaga agtggcagaa
    18541 gcctgcgttc agcctgcaca ctggagtggc gatgtggata cgctggcaga tatggtggtg
    18601 aaaaccgctc agcctggcga ccatattctg gtgatgagca acggcggttt tggtgggatc
    18661 catcagaaac tactggatgg tctggcgaag aaggcggaag ctgcgcagta attcggcctc
    18721 agcctgagat agtgtgcgta ggccggataa ggcgttcacg ccgcatccgg catttgagca
    18781 tagtgcctga tgcgacgctt gatgcgtctt atcaggccta caggtgcacc gcatccggca
    18841 aaccatactc acatcaacaa cgaaaattac ccttcattct ccgccagttc gcgcagatac
    18901 tggaaaatct ggcgtgcgga tttcggcggc ttattccctt ctttctcttt cttcgcgttg
    18961 cggatcagag tacgcagttg ctgacgatcg gcatcgggcc acagatttaa tacctcagcg
    19021 atggcgtcat caccttgatc gatcagacga tcgcgcaggt tttccagttt atggaacagc
    19081 accacctgct ggttgtgacg gtttttcagc ttatccagcg cctgacggat cggttctaca
    19141 tcgcgctggc gcagcatctt accgatgagc tgcaactggc ggcggcgacc ttccatctta
    19201 atacgctggg cgagctcaat agccgcgcgc agatccgcat ctaacgggat cttatccagc
    19261 gcgtttttcc ccagatcaac aatttccgcg ccaaggcgtt ttagctcctc ggcatcacgt
    19321 ttaatttcac ttttactgac ccagataatt tcatcgtctt cgtcttcgat gtcatcaccg
    19381 ggaacgtcgt cgagccagtc ttcgggctgc ttagtcatct caggctcctt aaaaaaagag
    19441 gctaatgtta ccagttaaga tgcgcactga aaaacggttc tctgttagac ttcagagaaa
    19501 ctctctacat tatggcactt gcaatgaaag taatctctca agttgaagcg cagcgcaaga
    19561 ttctggaaga agcagtttcc actgcgctgg agttggcctc aggcaaatcg gacggtgcgg
    19621 aagttgccgt cagcaagacc accggcatta gcgtaagcac gcgttatggt gaagtggaga
    19681 atgttgaatt caatagcgat ggcgcgctgg ggatcactgt ttatcaccag aaccgcaaag
    19741 gtagcgcatc atccaccgat ttaagcccgc aggccattgc ccgtactgta caggcggcgc
    19801 tggatattgc ccgttatacc tcgccagatc cctgtgccgg cgtggcagac aaagagctgc
    19861 tggcctttga cgcaccggat ctcgacttgt tccaccctgc ggaagtttcc ccggatgaag
    19921 ccattgaact ggcggcccgc gcagaacagg cggcattgca ggcggacaaa cgcatcacca
    19981 ataccgaagg tggcagcttt aacagccact acggtgtcaa agtttttggc aacagccacg
    20041 gcatgttgca gggttactgc tcaacgcgtc attcgctctc cagctgtgta attgccgaag
    20101 aaaatggcga tatggagcgt gattacgcct acaccattgg ccgcgcgatg agcgatttgc
    20161 agacgccaga gtgggtcggg gccgattgcg ctcgccgtac tttatcgcgt ctgtcaccgc
    20221 gtaaactctc caccatgaaa gcgccggtca tttttgccaa tgaagtggca accgggcttt
    20281 ttgggcatct ggtgggggcg atagcgggtg gatcggttta ccgtaaatct accttcctgc
    20341 tggattcgct gggtaaacaa attctgccgg actggctgac cattgaagag catccgcatc
    20401 tgctgaaagg gctggcatcg acgccatttg acagcgaagg tgtgcgcacc gagcgtcgcg
    20461 atattattaa agatggcatc ctgactcagt ggctgctgac cagctactcg gcgcggaaac
    20521 tggggctgaa aagcaccgga catgcgggcg gtattcacaa ctggcggatt gccggacaag
    20581 gtctaagctt cgagcagatg ctcaaagaga tgggcaccgg gctggtggtg acggaattga
    20641 tgggccaggg cgtgagtgca attaccggtg attattcccg tggtgcggcg ggcttctggg
    20701 tagaaaacgg cgaaattcag tatccggtta gcgaaatcac catcgcaggt aatttaaaag
    20761 atatgtggcg caatattgtc accgtcggta acgatattga aacacgcagt aatatacagt
    20821 gtggttctgt gctgttgccg gagatgaaaa tcgccggaca gtaaaaaaac tggcgcgacc
    20881 tgccgcgcta taaactaaaa aattacacaa aatcattcgc actgcatcga gacggaaagt
    20941 gaatgagggc cggggagcgt actcgcagta cgtgacccgg tcgaatgagc gtagtcgtcg
    21001 atgaggcagt gtgaaggatg aagtgtaaat aaaaaaggaa gtgagcaatg cgaaaaagcc
    21061 tgttagctat tcttgcagtc tcctcgttgg tattcagttc ggcgtcgttt gctgctgatc
    21121 ttgaagacaa tatggaaacc ctcaacgaca atttaaaagt ggtcgaaaaa gccgataacg
    21181 cggcgcaagt caaagacgcg ttaacgaaga tgcgcgccgc agcgctggat gcgcaaaaag
    21241 caacgccgcc gaagctcgaa gataaatcac cggacagccc ggaaatgaaa gatttccgcc
    21301 acggtttcga cattctggtc ggtcagattg acgacgcgct gaagctggca aatgaaggta
    21361 aagtaaaaga agcgcaggct gctgcagagc aactgaaaac gacccgcaat tcatatcaca
    21421 agaagtatcg ttaattcctc atttccctgt tgcctgcact caggtaacag ggaacttatc
    21481 atcgcaaatg atgcaccacc tgattgctgc tgccgcgcca gatcagggaa gggtctttta
    21541 aatcctgcac aaatttgccg tcgaccagca cgttaatcaa atcaacaacc tgcatttgcg
    21601 cagcgttgag ttcgtcgagt ttatagcctg tccacaccca gatgtcttta cccggacact
    21661 cggcgcggat gcgttgtacc agtttcagaa tatccggcac gttttgcgga tgcagcggat
    21721 cgccgccgga gagggaaata ccctggcgtt tgatacgagt gtcattcaga tcgttaatga
    21781 tctggtcttc cattgcttta gtaaatggct gaccggaatt tacccgccat gtgcttttgt
    21841 tatagcaacc ggggcattca tgaacacacc cggagacaaa cagggtgcag cgagtgccgg
    21901 gaccgttgac gatgtcgaca ggatagtact gatgataatt cattatttcg cctggcttat
    21961 cagtccgcgt gtaggctgta tcaggcaatc aacgcctgat gcgacgctgt cgcgtcttat
    22021 caggcctaca gccgttgcca aatgtaggcc ggataaggcg tttacgccgc atccggcaat
    22081 caacgcggga tgcgacgctt tcacatcaca tccggcattt gcttagtgca gatcaaccta
    22141 tctgcccatt tcccaaatgt ttaacgcggc gcttaacttc ttcctgcttg ccggcgttaa
    22201 acggacgtgc atccgggcta cctaaatatc cgcacacgcg gcgagttacc gacacacggg
    22261 aggcgtcatg gttaccacat ttcgggcagg tgaagccttt gctggtgcac tcgaactcac
    22321 cggtaaagcc acactcgtag cactcatcaa tcggtgtatt ggtgccgtaa tacggtacat
    22381 gctgatagct gtaatcccag acatcttcca gcgccttcag gttgtgctga atgtttggat
    22441 actcgccgta gcaaatgaaa ccaccgttcg ccagcggcgg gtaaggcgct tcaaagtcga
    22501 tcttgtcgta cgggttcacc ttcttctcca catcgaggtg gaaactgttg gtgtagtaac
    22561 ctttatcggt cacgcccggc accacgccaa actcagcagt atcgagacgg cagaagcggt
    22621 cgcacaggtt ttcactcggc gtgctgtaga gactgaaacc ataacccgtt tcttctttcc
    22681 actgatccac tgcctgacgc agacgttcaa caatggcgat acctttcgcg cggagctgct
    22741 cgttgtcgta aacatgttcg ccgccgaaca gcgcgttaat ggtttcgtgg atgccgatgt
    22801 aacccagcga aatagacgca cgaccgtttt tgaagatttc agaaatatca tcgtcagcat
    22861 tgagacgcac gccacaagca ccttccatat agaggatcgg ggccacgcgc gctttcacgc
    22921 cttcgagacg agcgatacgg gtcatcagag ccttacgtgc cagcaccaga cgttcatcca
    22981 gcagcttcca gaaggtggct tcatcgcctt ttgcttccag agcaatacgc ggcaggttca
    23041 ggctgatcac gccgaggttg ttacgaccat cgtggatctg ctcgccgttt tcattttccc
    23101 acacgccgag gaagctgcgg cagcccatcg gggttttaaa cgaaccggtg actttcacta
    23161 cctgatcgta gttcaggata tccggataca tgcgcttgct tgcgcactcc agcgccagct
    23221 gtttgatgtc gtagttcgga tcgccttttt tatggttcag accatcgcga atcgcaaaca
    23281 ccagtttcgg gaacaccgca gttttacggt ttttaccgag gcctgcgata cggttacgca
    23341 ggatagattc ctgaatcagg cgcgactccc agctggtgcc cagaccaaaa ccaaaggtta
    23401 caaacggcgt ctgaccgttg gcggtgtgca gggtgtttac ttcgtactcc agcgactgga
    23461 aggcatcgta gcactcttta atggtacgag agttagcgta gccttcggcg tccgggatgt
    23521 tccactcttc tgcggtcttg cgatgtttgt tgtagctggc ggtgacaaac ggtgccagca
    23581 cttcatcgat acggttaatg gtggtgccgc cataaatatg gctggcaacc tgagcaataa
    23641 tctgcgcagt taccgcggtt gccgtagaga tcgacttcgg cggttcaatc tcggcgttcc
    23701 ccatcttaaa gccctgggtc agcatgcctt tcaggtcgat caacatgcag ttgaacatcg
    23761 ggaaaaacgg tgagtaatcg agatcgtgat agtgaatatc gccacgctca tgtgcctgca
    23821 ccacgtcacg cggcagcagg tgctgacgtg catagtgttt agccacgatc ccggccagca
    23881 ggtcgcgctg ggttggaatc accttgctgt ctttgttggc gttttcgttg agtaacgagg
    23941 cgttggtctg ctcgaccaga ccacggatct cctgattcag gcgaccgcgt ttttcacgtt
    24001 caatgtcgcg atcgtgacgg tactcgatgt aagcacgagc cagttgtttg tatggacctg
    24061 acatcagctg gttttcaaca gcggtctgga tctcattgat atccacctgg ttgcggccct
    24121 gcatctgctc gctgacaact gcagcaacag tggcgcaata atcggcatca tcgacttccg
    24181 ctgctttagc tgcacgcaga atcgcttctt tgatgcgctc tgatttaaac ggcactttgc
    24241 agccgtctcg tttcatcaca tgcggtgtca tgatcgctcc atatttttaa gaacaggtta
    24301 tccacagaaa ttgggaaagg cgttttccgg ttttttcgtt tcgttttccg gtgctttccg
    24361 caatcgccat ccgcttatcc acaacgccgc acctttttcg tggggagctg tagtagcaat
    24421 tatagtcgat tgatacaaca tattgggttg ggacgcattt taaagtctat atatagtgct
    24481 ttgcatcaag gatgtttgaa ctttttttga tgtagctcaa agtaaaaagc agagcgtacg
    24541 gatgacgggt gctacagcgt tatgtaaatt ttttaatgaa tttgctggtt gaaaaatcaa
    24601 caaaaacaac atgctgacag acaaaacccc ggaatgaccg gcattaccgg ggcttaggga
    24661 agatttactt ctgtaaccac cagacagcct caaaaggccg taaagtcatg gcacagggtt
    24721 gtggtgaggc ttcttcgtag ttatgcatca caagctgcca gttgccgcgc atttgcccta
    24781 gctgccaggg ttggatctca cggctaaggt tggcaatgac cagcaaggtt tgccccttcc
    24841 attcacggcg atagcaccac aatacagggc tgtttggcag cagatcctgg taattgcccc
    24901 atgtcaggat ggcttcctgc ttacgcagtg cgattaactt ttggtaggtg taaaacaccg
    24961 aggactcatc ggccagcgcg gcttctacgt tgatttgttg atagttatcg cccaggccaa
    25021 tccacggttc gccagccgta aacccggcat tatcgccgtt gctccattgc atgggcgtgc
    25081 ggctgttgtc acgggattta ctggcgagga ttgccaataa ctcgtcggca tcacgcccat
    25141 cgttgcgcag ctcggcaaac atattgaggc tctctacgtc gcgatagtca gtaatgcgcg
    25201 tgaaatgcgg gttggtcatg ccaatctctt cgccctggta gatatacggc gttccctgca
    25261 tgccatgcag caccatcgcc agcatttttg ccgcaggcac gcggtattca ccttcatcac
    25321 caaagcgaga aacaatgcgc ggctgatcgt ggttacacca gaacaaggca ttcaatgcta
    25381 cgttgtgcat tccttgttgc cagtggcgga acaatgtttt caacgccaca aagtcaggtt
    25441 tagccagcgt ccatttttca ccaccgggat aatcgacctt caggtgatga aaattaaagg
    25501 tcatcgacaa ttcactgcct gtcagtgccg cgtatcgctg gcaatgctca aggctggtgg
    25561 aggacatttc acctacggtc attaacccgc gtggcgtaaa cacatcgcgg ttcatctcgt
    25621 gcaaaaactc gtgtgctcgt ggcccgtcgg tgtagaagcg acgcccgtcg ccatccaggt
    25681 catcagggaa acgcgggtct ttggagatca gattcaccac atccaggcgc aacccgtcga
    25741 ccccacgatc ggcccagaac tcacagactt ttttcagctc tgcgcgtacc gctggattct
    25801 cccagttgag atccgcctgt tctggtgcaa agagatgcaa atagtactgt tcgctttccg
    25861 catgccagcg ccacgcacta ccgccaaatt ttgaacgcca gttgttcggt ggcgtttctg
    25921 gttcgccatc gcgccagata taaaactggc ggtaagggct ttctttgttc agcgcctcgc
    25981 gaaaccaggc atgttgggta gaggtatggt taaacaccat atcgagaatg atacgaatcc
    26041 cgcgcgattt tgcctgcgtc accagttcgt caaaatcgtc cagcgtgccg taggtgggat
    26101 caatcgccgt atagttcgct acatcgtaac cgttatcgac ctgcggagag acataaaagg
    26161 gggttagcca gatggcatca acgcccagtt tatgcagata gtccaggcgt tggataacgc
    26221 cacgtaaatc gccggtgccg ctacccgtgg tgtcctgaaa actctttgga taaatctggt
    26281 agataacgcc gttttgccac cagtggggaa gattagtcat tacgttattc ctgcaaatgc
    26341 gagggggcgc aattgcgccc cgaagaaaat taaacaatgt ccagcgtgcc caggcggtat
    26401 ttccgctgat agataaacga ggtgagtaca atcgggatga tgatggcgat agccattgcc
    26461 agcgcaaaca cctgccagta gctcggttga atcgagagaa ttcccggcag gccgcctacg
    26521 ccgatgccat tcgccataac gccgttcagg ccgcatagca atcctgccag accagaacca
    26581 atcatcgcgc acagcatcgg gaagcgatat ttcaggttga tgccgtacat tgcaggctca
    26641 gtgaccccaa gccaggcgga gatcgccgca ggcacggaga tctcgcgttc attgtgcttg
    26701 cggctggaaa tgataatgcc tatcacggcg gagccctgag cgatattcga cagcgcaatc
    26761 agcggccaca ctggcgtacc acccatgctt tgaatcatct gcaaatcaat agcaagcgtg
    26821 gtctggtgta caccggtgat caccagcggg gcgtacagga agccaaacaa tgccgcgcca
    26881 atcggagcaa agctgccggt catcaggtga cgtaccgcaa aggcaacgcc atcgccaatc
    26941 atgcgaccaa acggaccaat cagcgcatgg gcgaggaaca ccgcgaggat cagcgaacag
    27001 acgggcacca ccaccagata gaggtaatcc ggcacgatgc gtttaaggcg agtttcaata
    27061 acgcccagcg ccagcccggc taacagtgcc gggatcacct gcgcctgata gcccactttg
    27121 gcgatgctga acatgccaaa gtcccacact tccggcagct gctgcccgag cagataagcg
    27181 ttcatcagtt ggggggaaac cagtgtcacg ccaagcacga taccaaggat cggcgtgccg
    27241 cccatttttt tcaccgctga ccagcaaatc ccgaccggca ggtagaagaa gatcgcttca
    27301 ccgatcaacc acagaaaatc gtagatcgtt tgcagggaag ggtacatttg cgccagcgtt
    27361 tgaccgttgc tcatgggcaa atcgccgatc acattgcgaa aaccgaggat caaaccgccg
    27421 ctaatcaacg cgggcagcaa cgggaagaag atctctgcga aatgagagat caactgctca
    27481 tgccatttca tattctgccg ggcggctttt tttacctgct ctttatcaac ctgcgcctgt
    27541 ccggttgacg ctatcagtgc ttgatagtaa tcacccacgt tggtgccaat caccacctga
    27601 aattgcccgg cattggtgaa acagcctttc accatgggga gttgctcaat ttctttcggt
    27661 ctggcattgg ccggttggtt gaggacaaag cgcaggcgag taatacagtg gctcaccgtc
    27721 gcaatattgc cgcgcccgcc gaccagttca atcaaccgat cgatatccgt ttggtttatt
    27781 ttgctcatca taaagcccca tggcagatga catttttggt tggctgcaga atatattgcg
    27841 cggaaaaatt taaaaacggg aacgttcccg aaacgcagcg aagatcacaa tttatcgttc
    27901 aggaaacgat caggacaggt tggcggggat gatgatttgt tgcggttcgc tgcgtccggt
    27961 tacctgcgcg atcaactggc aagccgcctg gcgtccagct tcggcgtaac caggatctac
    28021 ggtgacgatc tccggatgaa ggaatttcat taacggcgta ttaccgacgc tcgccagttg
    28081 caaggtgtcg atgcgttgct cttgcaggta tttacttgcg ccaagtgcca gcgtgtcggt
    28141 tgcgcacagt aaggcggtag tttcaggcgt aatcactttt gctacgttct catagccttg
    28201 cttcatagca agccctggca gggcggcaac gggatgcagt ttatgcgctt tgcagaacgc
    28261 caggtaggct tcgtgacgtc gcttaccggt tgtcacgtca ctgtgcggca cgccgagata
    28321 actgatatta cgatgcccct ggtcatacag ccgttgcatc aggatcttga ttgccccttc
    28381 gtcgtcataa cagaccgaag caaagccttt tgcgtcacgc gccagcagaa ccagcgatga
    28441 ctgccagtgg gctaacattt cttctgttat gccagtaaaa ccgaacagca ctacgccgtc
    28501 gatattacgc cgtttcagca cccccaaatg ttcggcaact aattgcgggg aaaactgact
    28561 ttccatcatg attgggtcgt aaccttgttc atagaacgct ggcagcatgg tttgaacggc
    28621 gagattttct gacaacgaat ccagacgggt aacaatgatg gcgaccactt tatcgctttg
    28681 cccacgcata gcgcgcgcag aacgggaagg ggaaaatcca tgctgattca tcactgcttc
    28741 aacacgctcg cgggtgcgct ggctcacgcc gctttcgtta ttcagcaccc gggaaactgt
    28801 agatttcccc acgccgctta agcgcgcgat gtctttgatg gtcagccgat tttgcatcct
    28861 gttgtcctgt aacgtgttgt ttaattattt gagcctaact ttacccgtgc attcagcaat
    28921 gggtaaagtc tggtttatcg ttggtttagt tgccagcagg tattatatcg ccatagatgc
    28981 tacgaatatt attggattct ccttattatt tgcggcgctt ttttcactta ccggaggtta
    29041 tatggaacct gatcccacgc ctctccctcg acggagatta aaacttttcc ggtaagcccg
    29101 tcttttcacg gcgttaccgg atgcgtaagg ccgtgacgtt ttaacgtccc tgctcagctt
    29161 tattaccttc aggtaaggct tcgccacgcc tgaagacatt tctgtactgt ttcagacagt
    29221 gcggagggac tccttatgtt taaagaaatt tttacccggc tcattcgcca tttaccttcc
    29281 cgtctggttc atcgtgatcc attgcctggc gcgcagcaga cagtgaatac ggtggtcccg
    29341 ccgtccttaa gtgcgcattg cctgaaaatg gcggtgatgc ccgaagaaga attgtggaaa
    29401 acgttcgaca cccatccgga agggttaaat caggcggaag tggaatctgc ccgcgaacaa
    29461 catggtgaaa ataaattacc cgcacaacaa ccgtcgccgt ggtgggtaca tttatgggtc
    29521 tgctatcgca acccctttaa tattttactc accattctcg gcgctatttc ttacgccacg
    29581 gaagatttat ttgccgcggg cgttatcgcg ctaatggtcg ctatttcaac gttgctgaac
    29641 tttattcagg aagcacgttc cactaaagcg gcagatgccc tgaaagcgat ggtcagcaat
    29701 accgcgacgg tgctgcgcgt aattaacgac aaaggcgaaa atggctggct ggagatcccg
    29761 atcgaccagc tggtgcccgg cgatattata aaactggcgg cgggagatat gatcccggca
    29821 gatttacgta tcttgcaggc gcgggatctg ttcgtcgctc aggcgtcgtt aaccggtgag
    29881 tctctgcccg tggaaaaagc cgctaccact cgccagccgg agcacagcaa tccgctggag
    29941 tgcgacacgc tgtgttttat gggcaccacc gtggtgagcg gcacggcaca agcaatggtg
    30001 attgctacag gtgccaatac ctggtttggt caactggcgg ggcgtgttag tgagcaggaa
    30061 agcgagccga atgcctttca gcaagggatc agccgcgtca gtatgctgct gattcgcttt
    30121 atgctggtga tggcaccggt ggtgctgtta atcaatggct acaccaaagg cgactggtgg
    30181 gaagcggcgc tgtttgcgct ttcggtcgcg gtaggcctaa cgccggaaat gttgccgatg
    30241 attgttacct cgacgctggc gcgcggcgca gtaaagctgt cgaaacagaa agtgatcgtc
    30301 aaacatctgg atgctattca gaactttggc gcaatggata ttctgtgcac tgataaaacc
    30361 ggcaccctga cacaggataa aattgtgctg gagaatcata ccgatatctc cggtaaaacc
    30421 agcgaacgcg tgctgcatag cgcgtggttg aatagtcatt atcagaccgg acttaaaaac
    30481 ctgctcgata cagcggtgct cgaaggtacg gatgaagagt cagcgcgctc gctggccagt
    30541 cgttggcaaa aaatcgatga gattccgttt gatttcgagc gtcgccggat gtcggtggta
    30601 gtggcagaaa ataccgagca ccatcagctg gtttgcaaag gtgcattgca ggaaatcctc
    30661 aatgtgtgtt cgcaggtgcg tcacaatggc gagattgtgc cgctcgatga catcatgctg
    30721 cgtaagatta agcgggttac tgatacgctg aatcgtcagg ggctgcgcgt ggttgcggtg
    30781 gcgacgaaat acctgccagc gcgtgaagga gattaccagc gggcggatga atccgacctg
    30841 atcctcgaag gatatattgc tttccttgat ccgccaaaag agacaaccgc tccggcactg
    30901 aaggcattaa aagcgagtgg aattaccgta aaaatcctta ctggcgacag tgagttagtt
    30961 gcagcgaaag tgtgccatga agtggggctg gacgcgggag aggtggtcat tggtagtgat
    31021 attgaaacgc tatctgacga cgaactggca aatcttgcac agcgcactac gctgtttgcc
    31081 cgcctgacgc cgatgcataa agaacgcatt gtgaccttac tgaagcgcga agggcatgtg
    31141 gttggcttta tgggcgatgg tattaatgat gcgcccgcct tacgcgctgc ggatatcggc
    31201 atttctgtgg acggcgcggt agatattgcc cgtgaagcgg ctgatatcat cctgctggaa
    31261 aaaagcctga tggtgctgga agagggggtt attgagggac gtcgcacttt cgccaacatg
    31321 ctgaaataca tcaaaatgac ggcgagttct aacttcggta atgtgttcag cgtgctggtg
    31381 gcgagtgctt tcttgccctt cctgccgatg ttgccgttac acttgcttat tcagaacctg
    31441 ctgtacgatg tgtcacaggt ggcgatcccg tttgataacg tcgacgacga gcaaattcaa
    31501 aagccgcagc gttggaatcc ggcggatctg gggcgcttta tgatcttctt cggaccgatc
    31561 agctcgatct tcgatattct gacgttttgc ctgatgtggt gggtattcca tgccaacacg
    31621 ccggaaacgc aaacgctgtt ccagtcggga tggtttgtgg tgggcttact gtcgcaaacg
    31681 ctgattgtgc atatgatccg cacccgccgt gtgccgttta ttcagagctg tgcatcgtgg
    31741 ccgttaatga tcatgaccgt gatcgtgatg attgtcggga tcgcattgcc gttttcaccg
    31801 ctggccagtt atctgcaatt acaggcgctg ccgttaagct atttcccgtg gctggttgcg
    31861 attctggcag ggtatatgac attaacccag ttggtgaaag ggttctatag ccgtcgttac
    31921 ggctggcaat aaagaataaa ctgggcacga tagccccgat tcagattgca gacaaagtaa
    31981 gctttgttca tgccggatgc ggcgtaaatg ccttatccgg ccaacaaatt caataaattg
    32041 caggaatcac gtaggcctga taagcgtagc gcatcaggcg attttgcgtt tgtcatcaga
    32101 cttaatccgg gcatgatagc ccggatttcc atcaagatta gcgacgaaca gcgatcgctt
    32161 cgatctcaat cttcacgtct ttcggcagac gggcaacttc aacgcaagaa cgtgccggga
    32221 aggtggcgtt gtgttcggtg aagaaggctt cgtaagtggc gtttacggtt gcgaagtcgt
    32281 tcagatcttt tacaaacacg gtagttttaa cgatgtcgcc cactttcaga ccagcggctt
    32341 cgacgatcgc ttttacgtta tccagcgact gacgtgcctg tgcagcgacg tctgccggta
    32401 cttcgcccgt tttcggattt accgggatct gaccggaagt gatgatcata ttgcccagat
    32461 caacgccctg tacgtaagga ccgatagctg ccggtgcatt ttccgtcgcg atagttttgc
    32521 tcatgatttc tcctttatta cagcggtaaa aagtctggct cattataggg agccagactt
    32581 ttattaccaa ccgcaattaa ttggccagca ccacattatg ggaaaactct ttttcacagt
    32641 atttgcattt gagcgcgata tcattggcgc gttttcgcac ggcaaagctg gaagaaaccg
    32701 gttcggcatg gctgatacag ttgctgttcg ggcagaccag cacattgtcg atgcgctccg
    32761 gcagacttgg gcgcgattta cccaccactt catagttgtc gatacggtta accgtggctt
    32821 gcggcgcata caatgccagt tgatctacct ggtcttcact caaaaaggta ttttcgattt
    32881 tgatcagatc tttgcggccc atctcgccag aaggcaggtt cagaccgatg gtgatgcgct
    32941 gatccgtttc ggtcagcttg aacagactca acagcttaaa accgatctgg gcggggatat
    33001 ggtcaattac cgtgccgcgt ttaatagctt caacctgcaa tttattatcg tgtgtcatct
    33061 ctatttcccc ttacagtacc agatcgcgat tcagaaccag tgccagtaac gcctggcgag
    33121 cgaaaatccc gttgcctgcc tgctggaagt accaggcgtg tggcgtttta tcaacatccg
    33181 tcgcaatctc atcaacacgc ggcagcggat gaagtacttt catattggct ttggcgttgt
    33241 ggagatcgct ggcgcgaaga acaaactgcg ctttcacgtt ggcgtactcg gacgggtcca
    33301 gacgctcttt ttgcacgcgg gtcatgtaca ggatgtcaac ttccgccatc acttcttcaa
    33361 tagagctgtg cagactccat gcgatccctt tttcatcaag catatccaga atgtattgcg
    33421 gcattgccag cgcgtccggc gcgatgaagt aaaaacggtt gccgtcgaac ttcgctaacg
    33481 cctgagtcag ggagtgaacg gtgcggccat atttcaggtc accaaccatt gcgacgtgga
    33541 gattgtccag acgcccctgg gtttcctgaa tggtgaataa gtccagcaag gtttgcgtcg
    33601 gatgttggtt ggagccatca ccggcattca gtaccggtac attgccggaa aactcggtgg
    33661 ccaggcgcgc cgcaccttcc tgcggatgac gcatcactat cgcatcgacg taagtgctga
    33721 taaccgaaat ggtatcggcc agcgtttcgc cctttttacc cagtgatgta ttggcgctgt
    33781 cggagaagcc caccacgctg gcacccaggc ggtgcatgga agtttcgaaa gagaggcggg
    33841 tacgggtaga ggcttcgaag aaacagctgg caatgacttt gtgcttcaac agctctggtt
    33901 gcgggtttgc tttcagtttc gccgctgtcg ccagcaccag attaaggtca tcgcgactaa
    33961 ggtcgtttat ggaaatgata tgtttctgat atagcggatt agccatcttt tatctcctga
    34021 cgcctgggca aaaaaaagcc cctcgattga ggggctggga atgggtgatc aacgggaaga
    34081 aaaacggcag gccagcgtct tttttcagac gcggtaagac aaaatgtcga acacactgaa
    34141 ccatacatcc tcccggcaaa ttgtccggca ttatactcat cgtcagaagc ggtgcaagca
    34201 tttgatgcaa tattttgtca gcgcaaacgg tttatttgaa ttataagtca aaatatatgc
    34261 atttttattt actgtgattc tggaggggaa ctgtcgttcg gtatcaataa ttgcagacat
    34321 ttcctgcttt tttctttttc actttcacgc aatcagatat gcattttatt ccattcatcc
    34381 gacttatagg gcggagtgtg aaaacgaacg gctaacacta ttgcttacag ctcagggatg
    34441 cgcgctatca ctttaatttc aaaatcaaag cctgccagcc atgtaacgcc caccgccgtc
    34501 caggttggat aaggtggcgc gctaaatatt tcatttttca ccgtcatgat gtcttcaaat
    34561 tgtttttctg gatcggtatg aaagctcgta acatcaatga tatcgtcaaa agtgcatccc
    34621 gcagctgcca gggtcgcatg caaattatca aatgccagtc tgacttgttg ctgaaaatcg
    34681 ggttctggtg ttccgtcctc tcgacttcct acttgcccgg aaacaaacag caaatcgccg
    34741 gaacgaatag ccgcagaata acgatgttct gcatacagtg aatgtcggcc agcagggaaa
    34801 acagcggttc tttctaccat ttggttatcc tcaagattta cgacatgaac agaagattta
    34861 tatttaccag gagccgcttt tagcggacga cgtgagtaaa caaaacccag acatcatggg
    34921 aaatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat caatggtatg
    34981 catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca taccagcgac
    35041 ctcttccggt tgcccatgtc ttttgatagc cataaaacta tgcaacatat cgcgcattgg
    35101 cccgttggcg ggattagcgt cggtatcaat tggccctggc tggacgacgt tgatggtgat
    35161 cccacgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgtaggg cagatttact
    35221 ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg agccgatgat
    35281 taagatgcgc ccaccttcgg gcatctgccg ggcggcttca acagaggcat ggtaaggagc
    35341 atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca gggcatcgcc
    35401 aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct tacgaacgac
    35461 atcaatgaca gcgtctctgt cagcactatc tgtaaatact gctgtcgctc ccgtctcttg
    35521 tgccaggcgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat tggccccatc
    35581 ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga ggatgagaac
    35641 tgtcttacct gtaaaagcgc ccataaggac tccttgattt attatgtaac atgcattaca
    35701 aaactgtttt aactttctgt caacaagttt tgtaatggtt actaaaaaac aatctcgcgt
    35761 tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa aagtgctttt
    35821 tcaccaaaaa ggtttcgatg ctgtcagtgt tgctgaagtt actgattatc ttggtattaa
    35881 ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc gtgtactcaa
    35941 tgaatatgtc ggtacggaag ctattccgct tgtcgatatt cttcgtgatg atcgtccggt
    36001 aggggagtgc ctggctgagg tattaaaaga agcggcgcgc agatatagcc aaaacggcgg
    36061 ctgcgctggc tgtatggttc ttgaaggcat tcatagtcat gatccacaag cgcgtgatat
    36121 tgccgttcaa tattatcacg ccgcagaaac ggccatttat gactatatcg ccaggctgca
    36181 tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag ggctttctgc
    36241 gaaggcacga gaggggcact ccctcgaaca actctgtgca actgctgcac tggcggggga
    36301 agcgataaaa actattctca aggagtgatg ctggccttga tccgaaaggc gggaacgcgc
    36361 ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggctga attgatcatc
    36421 ttttcgtata acggtggaaa tgaaacattg ttttaattaa ggagtggaaa agatgatcat
    36481 cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg cgattgagca
    36541 tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg aaggtaatcg
    36601 tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc gtgcggagta
    36661 ccatgcccgc tatctcgaca ttcagattgt gttaaaaggt caggaaggca tgaccttcag
    36721 cacacaaccg gcaggtacgc cggataccga ctggttagct gataaagaca tcgcattttt
    36781 gccggaaggc gttgatgaga aaacagttat ccttaatgaa ggtgattttg ttgtgtttta
    36841 tccgggggaa gtgcataaac cgctgtgcgc agtgggtgca ccagcccagg ttcgcaaagc
    36901 agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca ggtttacggc
    36961 gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt aatgcttaga
    37021 cgttcttatt aactcaagga gttcgtcatg agcaaaatat cagatttgaa ttattctcaa
    37081 cacattacat tagccgacaa ttttaaacaa aaaagtgaag ttttaaatac ctggcgtgtt
    37141 ggaatgaata attttgcccg taatgccggg gggcaggata acacaagaaa tatccttaat
    37201 cctaagacat ttttggagtt tttggtaaaa atatttaccc tgggttatgt ggattttagc
    37261 aaacgctcca acgaagcggg aagaaatatg atggctcata ttgagtcctc atcttatatc
    37321 aaaaataatg atggcagtga gataatgaag tttgttatga ataatcctga aggggaacga
    37381 gcggattcac ccaaggtgat tatagaaatt tcactttcca ctattactac tatggggact
    37441 cgtcaaggac atacagccat tatatttcca caacctgatg gttcgactaa ccgttatgaa
    37501 agaaagtcct ttgaaagaaa agatgagagt tcattacacc tgattactaa caaggttctg
    37561 gcgtgttacc aacgcgaagc taacaaggaa atagctcgtc tattaaataa tcatcagaag
    37621 ttaaataatc tacagaagtt aaataatcta cagaagttaa ataatataca gaagttaaat
    37681 aatatacagg agttaaataa ttcgcaggag ttaaataatt cgcaggagtt aaataactcg
    37741 caggacttaa aaaattcgca ggtgagttgt aaaggttcag ttgattttac gattacggat
    37801 ttattagaaa aatcattgaa taatgcatta ttagcaataa ggaacgaaca tctgctatta
    37861 atgcctcatg tatgtagtga atcgatttca tacttactgg gcgaaaatgg tatacttgaa
    37921 gaaatagata agctctacga attaaatgat cacggaattg ataatgacaa agaaggtaac
    37981 aatgaaatta atgacatcat gattaacctg tctcatattc ttattgaatc cttagatgat
    38041 gcaaaggtta atcttacacc ggtcatccat tcgatgttga tgactttttt agaattgcca
    38101 tataataatg atgtaaaaat actggagtgg tgttttaata aaagcatgca atattttgat
    38161 gattctgcaa agatagagca tgcatgctcc gtaataaatc atattaattt tcgtcgcgat
    38221 cagtctaaag tagctgagac attatttttc aatctcgata aagaacccta taaaaatagc
    38281 cctgaattac aggagttgat ttggaaaaag ttggttgtat atgtcaatga ttttaactta
    38341 agcaatcgag aaaaaacata tttaatacaa agaatattta ataatgttga gtcactattt
    38401 aataaagtac ctgtcagtat tttagttaat gatattttta tgaatgattt ttttatgaaa
    38461 aacactgaga tgattaattg gtacttccct cggtt
//
LOCUS       DABSYT020000036        38448 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.036, whole genome shotgun sequence.
ACCESSION   DABSYT020000036 DABSYT020000000
VERSION     DABSYT020000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.036"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(136..327)
                     /gene="yfgG"
                     /locus_tag="ID725_RS11210"
                     /old_locus_tag="pgaptmp_002242"
     CDS             complement(136..327)
                     /gene="yfgG"
                     /locus_tag="ID725_RS11210"
                     /old_locus_tag="pgaptmp_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HGV7850834.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            345..497
                     /locus_tag="ID725_RS11215"
                     /old_locus_tag="pgaptmp_002243"
     CDS             345..497
                     /locus_tag="ID725_RS11215"
                     /old_locus_tag="pgaptmp_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850835.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKSSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            679..2922
                     /gene="pdeF"
                     /locus_tag="ID725_RS11220"
                     /old_locus_tag="pgaptmp_002244"
     CDS             679..2922
                     /gene="pdeF"
                     /locus_tag="ID725_RS11220"
                     /old_locus_tag="pgaptmp_002244"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGV7850836.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2961..4502)
                     /gene="ppx"
                     /locus_tag="ID725_RS11225"
                     /old_locus_tag="pgaptmp_002245"
     CDS             complement(2961..4502)
                     /gene="ppx"
                     /locus_tag="ID725_RS11225"
                     /old_locus_tag="pgaptmp_002245"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGV7850837.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4507..6573)
                     /gene="ppk1"
                     /locus_tag="ID725_RS11230"
                     /old_locus_tag="pgaptmp_002246"
     CDS             complement(4507..6573)
                     /gene="ppk1"
                     /locus_tag="ID725_RS11230"
                     /old_locus_tag="pgaptmp_002246"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGV7850838.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6744..7382)
                     /gene="purN"
                     /locus_tag="ID725_RS11235"
                     /old_locus_tag="pgaptmp_002247"
     CDS             complement(6744..7382)
                     /gene="purN"
                     /locus_tag="ID725_RS11235"
                     /old_locus_tag="pgaptmp_002247"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGV7850839.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7382..8419)
                     /gene="purM"
                     /locus_tag="ID725_RS11240"
                     /old_locus_tag="pgaptmp_002248"
     CDS             complement(7382..8419)
                     /gene="purM"
                     /locus_tag="ID725_RS11240"
                     /old_locus_tag="pgaptmp_002248"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGV7850840.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8744..9370
                     /gene="upp"
                     /locus_tag="ID725_RS11245"
                     /old_locus_tag="pgaptmp_002249"
     CDS             8744..9370
                     /gene="upp"
                     /locus_tag="ID725_RS11245"
                     /old_locus_tag="pgaptmp_002249"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGV7850841.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9456..10745
                     /gene="uraA"
                     /locus_tag="ID725_RS11250"
                     /old_locus_tag="pgaptmp_002250"
     CDS             9456..10745
                     /gene="uraA"
                     /locus_tag="ID725_RS11250"
                     /old_locus_tag="pgaptmp_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGV7850842.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10840..11541
                     /gene="hda"
                     /locus_tag="ID725_RS11255"
                     /old_locus_tag="pgaptmp_002251"
     CDS             10840..11541
                     /gene="hda"
                     /locus_tag="ID725_RS11255"
                     /old_locus_tag="pgaptmp_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGV7850843.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11679..12038)
                     /gene="arsC"
                     /locus_tag="ID725_RS11260"
                     /old_locus_tag="pgaptmp_002252"
     CDS             complement(11679..12038)
                     /gene="arsC"
                     /locus_tag="ID725_RS11260"
                     /old_locus_tag="pgaptmp_002252"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGV7850844.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12059..13522)
                     /gene="bepA"
                     /locus_tag="ID725_RS11265"
                     /old_locus_tag="pgaptmp_002253"
     CDS             complement(12059..13522)
                     /gene="bepA"
                     /locus_tag="ID725_RS11265"
                     /old_locus_tag="pgaptmp_002253"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGV7850845.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13735..14796
                     /gene="yfgO"
                     /locus_tag="ID725_RS11270"
                     /old_locus_tag="pgaptmp_002254"
     CDS             13735..14796
                     /gene="yfgO"
                     /locus_tag="ID725_RS11270"
                     /old_locus_tag="pgaptmp_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGV7850846.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14834..15682)
                     /gene="focB"
                     /locus_tag="ID725_RS11275"
                     /old_locus_tag="pgaptmp_002255"
     CDS             complement(14834..15682)
                     /gene="focB"
                     /locus_tag="ID725_RS11275"
                     /old_locus_tag="pgaptmp_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGV7850847.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15704..17716)
                     /gene="hyfR"
                     /locus_tag="ID725_RS11280"
                     /old_locus_tag="pgaptmp_002256"
     CDS             complement(15704..17716)
                     /gene="hyfR"
                     /locus_tag="ID725_RS11280"
                     /old_locus_tag="pgaptmp_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGV7850848.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17746..18159)
                     /gene="hyfJ"
                     /locus_tag="ID725_RS11285"
                     /old_locus_tag="pgaptmp_002257"
     CDS             complement(17746..18159)
                     /gene="hyfJ"
                     /locus_tag="ID725_RS11285"
                     /old_locus_tag="pgaptmp_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGV7850849.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(18152..18910)
                     /gene="hyfI"
                     /locus_tag="ID725_RS11290"
                     /old_locus_tag="pgaptmp_002258"
     CDS             complement(18152..18910)
                     /gene="hyfI"
                     /locus_tag="ID725_RS11290"
                     /old_locus_tag="pgaptmp_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HGV7850850.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18907..19452)
                     /gene="hyfH"
                     /locus_tag="ID725_RS11295"
                     /old_locus_tag="pgaptmp_002259"
     CDS             complement(18907..19452)
                     /gene="hyfH"
                     /locus_tag="ID725_RS11295"
                     /old_locus_tag="pgaptmp_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGV7850851.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(19462..21177)
                     /gene="hyfG"
                     /locus_tag="ID725_RS11300"
                     /old_locus_tag="pgaptmp_002260"
     CDS             complement(19462..21177)
                     /gene="hyfG"
                     /locus_tag="ID725_RS11300"
                     /old_locus_tag="pgaptmp_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGV7850852.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(21167..22747)
                     /gene="hyfF"
                     /locus_tag="ID725_RS11305"
                     /old_locus_tag="pgaptmp_002261"
     CDS             complement(21167..22747)
                     /gene="hyfF"
                     /locus_tag="ID725_RS11305"
                     /old_locus_tag="pgaptmp_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGV7850853.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22752..23402)
                     /gene="hyfE"
                     /locus_tag="ID725_RS11310"
                     /old_locus_tag="pgaptmp_002262"
     CDS             complement(22752..23402)
                     /gene="hyfE"
                     /locus_tag="ID725_RS11310"
                     /old_locus_tag="pgaptmp_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGV7850854.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLSMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23414..24853)
                     /gene="hyfD"
                     /locus_tag="ID725_RS11315"
                     /old_locus_tag="pgaptmp_002263"
     CDS             complement(23414..24853)
                     /gene="hyfD"
                     /locus_tag="ID725_RS11315"
                     /old_locus_tag="pgaptmp_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HGV7850855.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24870..25817)
                     /gene="hyfC"
                     /locus_tag="ID725_RS11320"
                     /old_locus_tag="pgaptmp_002264"
     CDS             complement(24870..25817)
                     /gene="hyfC"
                     /locus_tag="ID725_RS11320"
                     /old_locus_tag="pgaptmp_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGV7850856.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25828..27846)
                     /gene="hyfB"
                     /locus_tag="ID725_RS11325"
                     /old_locus_tag="pgaptmp_002265"
     CDS             complement(25828..27846)
                     /gene="hyfB"
                     /locus_tag="ID725_RS11325"
                     /old_locus_tag="pgaptmp_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HGV7850857.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27846..28463)
                     /gene="hyfA"
                     /locus_tag="ID725_RS11330"
                     /old_locus_tag="pgaptmp_002266"
     CDS             complement(27846..28463)
                     /gene="hyfA"
                     /locus_tag="ID725_RS11330"
                     /old_locus_tag="pgaptmp_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGV7850858.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(28716..29186)
                     /gene="bcp"
                     /locus_tag="ID725_RS11335"
                     /old_locus_tag="pgaptmp_002267"
     CDS             complement(28716..29186)
                     /gene="bcp"
                     /locus_tag="ID725_RS11335"
                     /old_locus_tag="pgaptmp_002267"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGV7850859.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29186..29758)
                     /gene="gcvR"
                     /locus_tag="ID725_RS11340"
                     /old_locus_tag="pgaptmp_002268"
     CDS             complement(29186..29758)
                     /gene="gcvR"
                     /locus_tag="ID725_RS11340"
                     /old_locus_tag="pgaptmp_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGV7850860.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29904..30782
                     /gene="dapA"
                     /locus_tag="ID725_RS11345"
                     /old_locus_tag="pgaptmp_002269"
     CDS             29904..30782
                     /gene="dapA"
                     /locus_tag="ID725_RS11345"
                     /old_locus_tag="pgaptmp_002269"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGV7850861.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30799..31833
                     /gene="bamC"
                     /locus_tag="ID725_RS11350"
                     /old_locus_tag="pgaptmp_002270"
     CDS             30799..31833
                     /gene="bamC"
                     /locus_tag="ID725_RS11350"
                     /old_locus_tag="pgaptmp_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGV7850862.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            32046..32759
                     /gene="purC"
                     /locus_tag="ID725_RS11355"
                     /old_locus_tag="pgaptmp_002271"
     CDS             32046..32759
                     /gene="purC"
                     /locus_tag="ID725_RS11355"
                     /old_locus_tag="pgaptmp_002271"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGV7850863.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32927..33790
                     /gene="ypfJ"
                     /locus_tag="ID725_RS11360"
                     /old_locus_tag="pgaptmp_002272"
     CDS             32927..33790
                     /gene="ypfJ"
                     /locus_tag="ID725_RS11360"
                     /old_locus_tag="pgaptmp_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGV7850864.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33805..35820
                     /gene="tmcA"
                     /locus_tag="ID725_RS11365"
                     /old_locus_tag="pgaptmp_002273"
     CDS             33805..35820
                     /gene="tmcA"
                     /locus_tag="ID725_RS11365"
                     /old_locus_tag="pgaptmp_002273"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGV7850865.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35894..36592
                     /gene="ypfH"
                     /locus_tag="ID725_RS11370"
                     /old_locus_tag="pgaptmp_002274"
     CDS             35894..36592
                     /gene="ypfH"
                     /locus_tag="ID725_RS11370"
                     /old_locus_tag="pgaptmp_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGV7850866.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36673..36873)
                     /gene="ypfN"
                     /locus_tag="ID725_RS11375"
                     /old_locus_tag="pgaptmp_002275"
     CDS             complement(36673..36873)
                     /gene="ypfN"
                     /locus_tag="ID725_RS11375"
                     /old_locus_tag="pgaptmp_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGV7850867.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36901..38028)
                     /gene="dapE"
                     /locus_tag="ID725_RS11380"
                     /old_locus_tag="pgaptmp_002276"
     CDS             complement(36901..38028)
                     /gene="dapE"
                     /locus_tag="ID725_RS11380"
                     /old_locus_tag="pgaptmp_002276"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGV7850868.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38032..38388)
                     /gene="yffB"
                     /locus_tag="ID725_RS11385"
                     /old_locus_tag="pgaptmp_002277"
     CDS             complement(38032..38388)
                     /gene="yffB"
                     /locus_tag="ID725_RS11385"
                     /old_locus_tag="pgaptmp_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGV7850869.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
BASE COUNT         9386 a         9920 c        10112 g         9030 t
ORIGIN      
        1 acaggataaa catcagtcgg gacaaagacg tataatttaa gatgaaagaa tatctgccgc
       61 tttgataatt ccaaagagca aaagcatccg acagcgaagc gccgttatgt tctgtgaaag
      121 tggagaaggt aaccgctaac gttgtaccgg tgattcgacg gagagtgtgg attgctgagc
      181 ttcttttttg ctctgatggt gctgccaggc accgacggac gagtagataa aacggccaaa
      241 gaagaagata aagctgatga gcagtacgat acgggtcatg cgactgttaa atcggtgtcg
      301 ttttcgcata ctggttgcct gactcacaaa aggttccttg aagtatgtcc cacgccgtgg
      361 acggtactta cattaaggca caacaggaca aaatggtcaa ttcttcgtta tgtaaaaaat
      421 cgtcagtgga tacatatttt aatgttatgg aagttaattt aattatttac aatgatgatg
      481 taataatgat gaattaacat gataaacgta ataattcatt aatcagggtt attttgtttg
      541 atatatatca attggaactt tcatatgaca cttagaatca atgctctcta tagtgatgaa
      601 taatcatcat tcgaagtcag gtgggatgcc tgtctgaata cacctccttc aggatgtggg
      661 ggattctgct gagcatctat gaaactgaat gcaacttata taaaaatacg tgataaatgg
      721 tgggggcttc cgctgttcct gccttcttta atcttgccca ttttcgccca cattaatact
      781 ttcgcgcata tttcttccgg tgaggttttt ctcttttatc tgcctctggc actgatgatc
      841 agcatgatga tgtttttcag ctgggcggca ttgccaggga tcgccttagg gatttttgtc
      901 cgcaaatatg cagagctggg tttttacgaa acgctctcat taacggctaa ttttattatc
      961 attatcattc tctgttgggg cggttacagg gtctttactc cccggcgtaa caacgtttca
     1021 catggtgata cccgtttaat ttcccagcgt atattctggc agattgtgtt tcctgcaacg
     1081 ctgtttctga tacttttcca gtttgctgca tttgtaggat tactggcgag cagagaaaat
     1141 ctggttggcg tcatgccttt taatctcggg accttaatca attatcaggc cttactggtg
     1201 ggcaatctga ttggtgtccc gctgtgctac ttcatcattc gggtagtgcg aaatccgttt
     1261 tatttacgta gttattattc gcaattaaaa cagcaggttg atgtcaaagt caccaaaaaa
     1321 gagttcgcac tctggctgct ggcattaggt gctttactgt tgctgttatg catgccgtta
     1381 aatgaaaaaa gcacaatttt tagcaccaat tataccttgt cattattgct gcccctgatg
     1441 atgtggggag cgatgcgcta tggttataag ctgatttcgc tgctctgggc ggtcgtgttg
     1501 atgatcagca tccacagcta tcaaaattac attcccattt atcctggcta taccacgcag
     1561 ctgaccataa cctcctccag ttatctggta ttctctttta ttgtcaatta tatggctgta
     1621 ctggcaaccc gtcagagaac ggtagtcaga cgcattcagc gccttgcgta tgtggacccg
     1681 gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt tgcgtgatgc cccctggtct
     1741 gcgctttgtt atttacgcat ccctggcatg gaaatgctgg ttaaaaacta tggcatcatg
     1801 ctgcggattc aatacaagca aaaactttct cactggctgt cacccttgct ggaaccaggt
     1861 gaagatgttt atcagctttc gggtaacgat ctcgcgctgc gactgaatac agaatcgcac
     1921 caggagcgca ttaccgcact ggatagccat ctcaagcaat ttcgtttctt ttgggatggc
     1981 atgccgatgc aaccgcagat tggcgtcagt tactgctatg tgcgctcgcc agtgaatcat
     2041 atctacctgc tgctgggaga gctaaatacg gtcgccgaac tttccatcgt gaccaacgcc
     2101 ccggaaaata tgcagcgtcg cggggcaatg tatttgcaac gcgaattgaa agataaagtc
     2161 gcgatgatga atcgactaca gcaggcgctg gaacacaacc attttttcct gatggcccag
     2221 ccgattaccg gtatgcgtgg tgatgtttac catgaaattc ttctgcgcat gaaaggtgag
     2281 aatgatgaac taatcggccc cgatagcttc ttgccggtcg cgcacgaatt tggtttatcg
     2341 tcgagtatcg acatgtgggt cattgagcat acgctgcaat ttatggctga aaacagagcg
     2401 aagatgcccg ctcaccgttt tgctattaat ctgtctccaa cctcggtatg tcaggctcgt
     2461 tttcctgttg aagtcagtca gttgctggat aaatatcaga ttgaagcgtg gcaacttatt
     2521 tttgaagtca ccgaaagtaa tgctctgacc aatgttaagc aggcgcaaat caccttgcag
     2581 catcttcagg aattaggctg ccagattgcg attgatgatt tcggcaccgg ctacgccagc
     2641 tatgcgcggc ttaaaaatgt gaatgccgat ctgcttaaaa ttgacggcag ttttatccgc
     2701 aatattgtgt caaatagtct ggattatcag atagtggcat cgatttgcca cctggcgcga
     2761 atgaagaaaa tgcgggtagt ggcagagtac gttgaaaacg aagagatccg cgaggcggtg
     2821 ctctctttgg ggatcgatta tatgcagggt tatcttattg gtaagccgca accgttaatt
     2881 gatacgctga atgaaatcga acccattcgc gaaagtgcct gaataatgcg ggccgacatt
     2941 tctcgtcggc ccgcaaagca ttaagctgcg atttcaggtg tactttcttc ttcaattttc
     3001 aaccgccagc cagccacgcc ttcccagtat tcttgctcct tttccagatc aagcagtacc
     3061 agcgcattct gactaaacca gtcatgcggg aaacgcagtg tccagtgact gtcatcagta
     3121 atcagcgtca atgttggcgg tgtggtggtt gcctgacgct gattgttgag taatacgcca
     3181 aggcgcaata gctgaatcag tggcaggaac tgtttcttct taaacaaggt aaagcgcggc
     3241 agatcgtcga gtttaatcgc tttacggtga tagcgcacca gtgtcgccat catcagctgc
     3301 tgttcctgat taaaacccgg caagtcgctg ttttgtagaa tataagcgga gtgacgatgc
     3361 aaaccgctgt ggttgatatt caacccgacc tcatgcagca tggcggccca tcgcagtagc
     3421 gcctccagtt gcggatgcgc cagcttcggt tgctgttccc gccactgttc gtacatttgc
     3481 atagtggtat ccagcacccg tcgggcctgt tcgctgtcga tgtgatactg gttggcgagg
     3541 ctgctggcgg tgcgactacg cacatcctga tgacggaaac gtccttccat ttcatacagt
     3601 acgccttcgc gtaacgcccc gtcagaaagg cgcagttcac ggatggctaa agcatcaaac
     3661 acaccgcata aaatcgctag tccaggaacg aagactgttt tccgctcttc ggaaagacct
     3721 ggtaaactca gcgatgcgaa attacggtgc cgtaaaactt cttttaccag tttttccaga
     3781 cgttccgggg taattatccc gtctttctcg cccatttcca ttaacacttc atgggcggct
     3841 tttatggtac cggaagcgcc cattgccacg ttccagccct gaatacgaaa ttgccaggtt
     3901 aaagtttcca gtttttgtgc tgccgccatg cgagcgcgct gaaaattctc tttattgatg
     3961 accccgccag ggaaataaag ctgggcaaag ctgacacacc ccatccggcg gctttcaacg
     4021 agaatcggtt cgaaattttc accaatcacc agttctgtag aaccgccgcc aatatcaata
     4081 accagtttgc gacctttttc cggttgggta tgttccacgc ccataaaaat cagacgggct
     4141 tcttcattac cggaaataat ttcaatcggg tagggaatga ccttttccgc acgtttcaga
     4201 aagtcagtgg cgttcagcgc ctgacgcagc gtatgggtac caactataca gacgctggca
     4261 ggagaaaacc cttgtagccg ttcggcaaac agcgacagac agttcaaacc gcgcgtcatt
     4321 gcctcttcac tcaacatatt atctggcccc aggccgtccg ccagatgcac ccgctgtttc
     4381 aggcggccaa taatctgcat ggcaccatct accacacggg ctatgaccat gtgaaaactg
     4441 tttgaaccaa gatcgaccgc agcaaactcc tgcggacgag gggatttatc gtgtattggc
     4501 atagggttat tcaggttgtt cgagtgattt gatgtagtca taaatcgcca actgcgcccg
     4561 tactttgcgg cgattgccgc ggggaacgta gcgattactg agttctttat cgatataacg
     4621 tgctttgacc gtatcgctga acaatatgtc gatgatgtcc agcacccgct gcttcaggcg
     4681 cggatcgagc agcggcgtcg ccacttcaat acgataatca atattgcgcg tcatccagtc
     4741 ggcggaagaa aggtagacct ttttatcgcc gccattttca aaaatataaa cccggtcatg
     4801 ttcaaggtaa cggtcaacaa tactgatggc acgaatgttg tcgctaatgc cttccagatt
     4861 ggggatcagc gaacacattc cgcgaaccag cagattaacc ggtacgccgg agctggaggc
     4921 cgcatacaga cgatcaacca ggcctttatc gacaaggtta tttagcttca gggtgatacc
     4981 actgggcagc ccttgctgcg cgttggcgat ctcgcggtcc accatttcat acaacaggcg
     5041 gcgggagttt tgcggcgaca ccattaaata atcaaatgtc accggacggt atgggttttc
     5101 aataaagtta aatacccgcc gtacttcgtt ggtgatgcgc gcatcggcgg tcagcaacga
     5161 atagtcagta taaagacgcg cggttttttc gttaaagttc ccggtcccga tgtgtgcata
     5221 acgcaccact tcaccgtttt ctttacgtga aatcagaaac agtttggcgt gaattttcag
     5281 ccccggcgca gagaagataa cgtgcacgcc tgcttcggtc aggcgcttcg cccagtgaat
     5341 gttggcttct tcgtcaaaac gtgcctgtag ctcaaccacc acggtgactt tcttaccgtt
     5401 gtgtgcggcg tggatcatcg agtcgatgat gcgtgaatct tttgccacac ggtaaatgtt
     5461 gattttaatc gccagcacgc tcggatcgaa cgaggcctga cgcagcagtt ccagcacatg
     5521 ctcaaaggtg tgataaggat aatagagcaa cacatcgcgt tcgcgaatgg catcaaaacc
     5581 attgcggaac tgggctttat caaaccaaat atggcgtaaa cgcggcagtg gtttgttcac
     5641 cagattggct ttgccgacat tggggaaatt aataaagtct ttaaaattat gataacgacc
     5701 gccggggacg atggagtcgt agcgggaaat agtcagtttt tcgcgtaaca cttcaaccag
     5761 cgcattgggc atatcgcgct gataaacaaa acgcaccggc tcagcagtta aacgctgctt
     5821 gagactggaa gacatcaact ccatcaggct ggcttccatc tcatgcacta aatcgtattc
     5881 ggcatcgcgg gtcatcttca ttgaataggc attcagcgcg tcataatcaa agaagccttt
     5941 gaaaatatca tcaaggcagt aacgcagaat gttatccaga agaatcattg gcttgcgtcg
     6001 acgcggcgct tctggcggta aattcacaaa gcgcggcact ttatctgatg ggatctccag
     6061 aagcgcgtaa cggatggtat cgccacggat aatttccacc gccagatagg tgtaatcatc
     6121 tttcaggaac tgcactaagt cagtgtcagg attgattaaa atcggcgtaa tgtgctgacg
     6181 cagatactgc ttaaaataat gacgcagcca gttttgttga ttgacggaga gctggcgttc
     6241 attaatcagg aagatctggt tgcgcgccat ctccagcaat agctcgttgt agaggccgtc
     6301 gaattcctga tcggctttca gcacccggga ctgaattttg cccagtaaat ggcgggaatg
     6361 agagttggag ccttgttctt cgctaataat gatgcgtcgc ttcagttcag cgaagcggac
     6421 tttatagaac tcatcaaggt tattggaata gatccccagg aaacgcatcc tttcaatcag
     6481 cgggttagat ttgtccgccg cttcctgaag cacgcgttca ttgaacgata accaactgag
     6541 ctctttttcg atgtatagct tttcctgacc cattaccact tttactccgt tttattcacg
     6601 ggacactgcc tggatattat ggcgagcaat attggcagat gtccaactat gccagaaaag
     6661 ggggaaaaaa actgctggag cttgcgattt acaggtgaat tgaaaaacgc cagcggcaga
     6721 gctggcgctt taactacggg gcattactcg tcggcagcgt agccctgcgg cggcagacgt
     6781 tgaccatcca gccacgcggc gttttcgtgc attttcagac gaccatcggc aaaccagcta
     6841 atcaccagtg gataaatggc gtgttcctgg gtttgcacgc gggcggtgac gtcatcctcc
     6901 gtatcaccag caaataccgg gactttcgcc tgtaaaataa ccgggccacc gtccagttca
     6961 tcggtgacga aatgcaccga tgtaccgtgc tcttcatcgc cattttccag cgcctgacga
     7021 tggggatgta atccgggata tttcggcagc agagaagggt gaatgttcag caaacgcccg
     7081 gcatagtggg agacaaatgc cgggctgaga atgcgcataa aaccagccag cacgaccaca
     7141 tcgggtgcgt acatgtcgat ttcatgaatc aactcccggt cataggcttc acgactgtca
     7201 aacgcgctgg cgatgagcgt atgcgttgca atacccgcct ggcgggcgcg ttcaaggccg
     7261 aacgcgtcgg ccttattgct gaaaactgcc cgtacggtgc ctttaatttt gttggtttta
     7321 caggcgtcaa taattgcctg caaattactt ccgttgccgg aaataagcac cacaatattc
     7381 attattcgat aaccacgcgt tgttcggaat cagaggcttt gatgataccg attttccacg
     7441 cgttttcacc gttggcattg agcagggcga gggctttgtc cacttccgga gcaggcaggg
     7501 caataatcat cccgacgccg cagttgaagg tgcgatacat ttcatggcgc tcaacgttac
     7561 cagccgtttg cagccagttg aacacttccg gccactgcca ggaggattca tcaatcactg
     7621 cctgggtatt atctggcaat acgcgcggaa tgttttccca aaagccgccg ccggtcaggt
     7681 gcgcaatggc attaacatcg accttttcaa tcaactccag cactgacttc acgtaaatgc
     7741 gggtcggtgc cagcagatga tcggctaatg gcttaccatc aagttcggtg gtttgcggat
     7801 cacaaccgct gacttcaaga attttgcgca ccagcgaata gccgttcgag tgcggaccgc
     7861 tggaaccgag tgcaatcagc acatcgccgt cgctgacttt agagccgtcg atgatttctg
     7921 atttttctac cacgcccacg cagaaacccg cgacatcgta atcctcaccg tgatacatcc
     7981 ccggcatttc tgccgtttcg ccacccacca gtgaacagcc cgattgcaga caaccttccg
     8041 caatgccgct gatcaccgct gaagcggtat caacatccag ttttccggtt gcgtaatagt
     8101 cgaggaaaaa cagcggttct gcaccttgca ccaccaggtc attaacgcac atggcgacca
     8161 gatcaatacc aatggtgtcg tgacgtttta agtccattgc cagacgcagc ttggtaccta
     8221 cgccgtcagt gccggaaacc agcacgggtt cacgatattt ttgcggcaat gcacacagcg
     8281 caccgaagcc gcccagaccg cccatcactt ccggacgacg cgttttcttc actacgcctt
     8341 tgattcttcc aaccagagca ttacccgcgt caatatcaac accggcatct ttgtagctaa
     8401 gagaggtttt atcggtcact gcttgggtcc ccacgcgtta cttgcggtag aaaaataaaa
     8461 ttcggcgcaa ttctaacagg gaaagcaaac gtttgcgaga ctgctttaca caaccttttt
     8521 gcacgtcttt tccccaggcg cgcggcgaaa gaagacttgt gccagggcaa aggttagttt
     8581 tcggatggaa taatcttctt tcataaccat ttgaatataa aataacttta tctcaaaccg
     8641 ttatcatttt gactaaagtc aacgaaaata atattgccgc cttgaagaaa ggaggtataa
     8701 tccgtcgatt ttttttgtgg ctgcccctca aaggagaaag agtatgaaga tcgtggaagt
     8761 caaacaccca ctcgtcaaac acaagctggg actgatgcgt gagcaagata tcagcaccaa
     8821 gcgctttcgc gaactcgctt ccgaagtggg tagcctgctg acttacgaag cgaccgccga
     8881 cctcgaaacg gaaaaagtaa ctatcgaagg ctggaacggc ccggtagaaa tcgaccagat
     8941 caaaggtaag aaaattaccg ttgtgccaat tctgcgtgcg ggtctgggga tgatggacgg
     9001 tgtgctggaa aacgttccga gcgcgcgcat cagcgttgtc ggtatgtacc gtaatgaaga
     9061 aacgctggag ccggtaccgt acttccagaa actggtttct aacatcgatg agcgtatggc
     9121 gctgatcgtt gacccaatgc tggcaaccgg tggttccgtt attgcgacca tcgacctgct
     9181 gaaaaaagcg ggctgcagca gcatcaaagt tctggtgctg gtagctgcgc cagaaggtat
     9241 cgctgcgctg gaaaaagcgc acccggacgt cgaactgtat accgcatcga ttgatcaggg
     9301 actgaacgag cacggataca ttattccggg cctcggcgat gccggtgaca aaatctttgg
     9361 tacgaaataa agaataaaaa taattaaagc cgactttaag agtcggcttt tttttgagta
     9421 aagcgcctat aacacataat acagaggata atactatgac gcgccgtgct atcggggtga
     9481 gtgaaagacc gccactttta cagacaatcc cgcttagttt gcaacatttg ttcgccatgt
     9541 ttggtgcaac cgtcctggtg cccgtcttat ttcatattaa cccggcgact gtactgttat
     9601 ttaacggtat tggaacgctg ctgtatctct tcatctgtaa agggaaaatt ccggcttatc
     9661 ttggttccag ctttgccttt atttcaccgg tattgttact gttgccgtta gggtatgaag
     9721 tcgcgctggg cggctttatt atgtgcggcg tgctgttctg cctggtttct tttatcgtga
     9781 agaaagcggg gaccggctgg ctggacgtgc tgtttccacc tgcggcaatg ggcgcaatcg
     9841 ttgccgtcat cggtctggag ctggcgggcg tagctgccgg tatggcgggt ttactcccgg
     9901 ctgaagggca aacgccagac tccaaaacca tcatcatctc tattaccacc ctggcggtca
     9961 cggttttagg ttccgtgctg ttccgtggtt tcctggcaat tatcccgatt ttaattggcg
    10021 tgctggtggg gtacgcgctc tctttcgcaa tgggaattgt cgataccacg ccgattatta
    10081 atgctcactg gtttgcgctg ccaaccctct atacgccgcg cttcgagtgg tttgccattc
    10141 tgactattct gccagcggcg ttagtggtta ttgccgaaca cgtagggcac ctggtagtaa
    10201 cggctaatat cgtcaaaaaa gatctgctgc gcgatccagg tctgcaccgt tcgatgtttg
    10261 ctaacggctt gtcgaccgtg atctccggct tctttggctc cacgccaaac accacctacg
    10321 gggaaaacat tggcgtgatg gcgatcaccc gtgtttacag tacctgggtt atcggcgggg
    10381 cggcgatttt cgctatcctg ctttcctgcg tcggtaaact ggctgccgct atccagatga
    10441 tcccattgcc ggtgatgggc ggcgtttcgc tgctgcttta tggtgtcatc ggtgcttccg
    10501 gtattcgtgt tttgatcgaa tcgaaagtgg attacaacaa agcacagaac ctgatcctga
    10561 cttccgtgat tttgatcatc ggcgtcagtg gcgcgaaggt aaacattggc gcggcggagc
    10621 tgaaaggtat ggcgctggcg accatcgtcg gtatcggctt aagtttgatt ttcaaactga
    10681 ttagtgtgct gcgccctgaa gaagtggtgc tggacgcaga agacgcggat ataacagaca
    10741 aataatgtac cgaccgggca gtgttgctgc ccggttcaaa catcatggga ttctgtggta
    10801 aacttctcgc gattttgtga aatcctggtt gaggtatctc tgaacacacc ggcacagctc
    10861 tctttgccac tttatcttcc tgacgacgaa acctttgcaa gtttctggcc gggggataac
    10921 tcctctttac tggccgcgct gcaaaacgtg ctgcgtcagg aacatagcgg ttacatctat
    10981 ctctgggcac gcgaaggcgc ggggcgcagc catctgctgc acgcggcttg cgcggaattg
    11041 tcgcagcgtg gcgatgcggt gggctatgtc ccgctggata aacgcacctg gtttgttccg
    11101 gaagtgctcg acggtatgga gcatttgtcg ctggtctgta tcgacaacat tgagtgtatt
    11161 gcaggcgatg agttgtggga gatggcgatt ttcgatctct acaatcgaat tctggaatcg
    11221 ggcaaaacac ggttgttgat caccggcgat cgtccaccgc ggcagttgaa tctgggatta
    11281 ccggatctcg cgtcgcgact cgactggggg cagatctaca aattgcagcc actttctgat
    11341 gaagataagt tgcaggcgct acagttacgc gcgcgtttgc gtggttttga actgccggaa
    11401 gatgtggggc gtttcttgct gaagcggctc gacagagaaa tgcgcacgct atttatgacg
    11461 ttggatcagt tggatcgtgc gtcgattacc gcgcaacgta agctgaccat tccgtttgtg
    11521 aaagaaattc tgaagttgta gataaggtgt ttattgtcgg atgcggcgcg aacgccttat
    11581 ccgaactacg cgaagttaat ccatagaatt cgtaggccgg ataagacgtg gcaagcgtcg
    11641 catccggcaa tgtgcttcaa ctagcgatgg agcacatctt aacccacgat ttccagtacc
    11701 tgctccggtg gacgaccaat ccgcgctttg ccattcgcga ccacaatcgg gcgctccatc
    11761 agcttcggat tatccaccat tgcctgaatc agcgcctctt cgctaagcga actgtcagcc
    11821 aggttcagtt ctttataaag atcctctttc tggcgcatca attcacgggc gctattcatc
    11881 cccagcattt tcagcaaatc gcgcagcgtt gccgcatcgg cgggtgtctc aaggtagagc
    11941 accacttctg gctccacgcc gttttctttc agcaggttca gcgtttcccg gctctttgaa
    12001 cagcgtgggt tatggtagat ttttacctgt ttggtcattg ttcttccttt aatgcgaatt
    12061 acatcttggt ataaggctta aagcgttcct gcagctggcg caactggtcg atgcgcgcat
    12121 cgtaacgcgc ttgttgcagg ctgcctaatt tcacctgcga actggcgcta ctcaacagcg
    12181 aaatggcctg atcgagtcgt ccggcgagcg cataaccttc tgctcgcgca gccagctcct
    12241 gatcgcggtt atttagcgcg gcttccgcct gtgccagcaa atcccagccg ttgctgtcat
    12301 ctttattatt aaaggtgtag cgattaagaa tattggccgc ttcttgtggt tgaccgcctt
    12361 gtagataagc gttcgccagg ttgagctgca acacaggatt ggtgcgcaaa tcgcgggcat
    12421 ttttcagacg attgatcgcc tcattggctt tgttttgccc aagatcgata tcagtagcca
    12481 gatcgagata ccatgcgttg ccaggttctg ccgccagtaa cggttgcagc gtttttcgcg
    12541 cctcgtcgta tttattggct tccatcgcct gtaaagcacg accatattgc gccgctcgtt
    12601 gctgacgaac gtttcctttc gcccattcat ccagcaaatc actggtgagc tggttacgtc
    12661 cggaattata catccccagt gtgcgcgctt tcgccagata gaaatcttcc gacgactgca
    12721 ccaccatcgg gcgcatctga ttagcacggt tgcgggcatc tgccagacga ctttccggca
    12781 acgggtgagt cagtaaaatt tccggcgggc gcgaggagta acgcgcctga tcgagtaatt
    12841 tttcgaggaa ggttggcatc gcctgcggat cgaatcccga gcgttgcagc acctgaatac
    12901 caatgcggtc cgcttcctgt tcattttgct gggtgaaact gatcatcccc tgacgcgttc
    12961 ccgccagtgt accggtcagc gccgccatcc ccgcctgcgg actggccatc gccagtaaaa
    13021 tagaacctaa cgcgccgacc caggtcagcg gcgcgttgcg ctgctgatct tccatcgctc
    13081 gcgccaggtg acgttgggtg acgtgggaga tttcgtgcgc cataactgaa gccagttgac
    13141 tttcgttatc ggaataacgg aacagggcag agtgcagcac cacgttgccg ccaaagaaag
    13201 caaaggcgtt aatttcgtcg ttgttgatca gaaagaaatg aaacggtgtc ttaaccgaat
    13261 tggcatgcga aaccagacgc atccccagcg aattaatata ttgcgttaac agcgggtcat
    13321 taattaacgg cgcgctgccg cgtagctggc ggacataata gtcgcccatc tgcatttcct
    13381 gaccaatgga aagcgtgctt cctgcggagg ttcccatatc cggcaaggtg tctgcgctgt
    13441 cggcaaatgc cggggctacc tgaccaatgg tcatagcagc aatgagggtt gcaaccaggt
    13501 tttttttcaa ctgcctgaac ataacctcta tcctgtattt ctgaagatag tcatttgacc
    13561 gatactgtga aatatcgttc ccaccgcgcc gcctttgagt gtaacggcga agattagccc
    13621 actcaaatga cttttcagtt tcctgaatcc gtcataaaaa gcgaagtctt ttttgtgaca
    13681 tttcgataca attgcggatc gcaatcccgc tcttgaggtt cagggaaggt ctttatgctc
    13741 gaaatgttga tgcaatggta tcgccgccgt tttagcgacc cggaagcgat tgccttgctg
    13801 gttattttag ttgccggatt tggcattatc tttttcttta gtggcctgct tgctccgttg
    13861 ctggtggcta ttgtgctggc ctatttgctg gaatggccaa ccgtgcgcct gcaatctatt
    13921 ggctgctccc gccgctgggc gacgtcgatt gtattggtgg ttttcgtcgg tatattgcta
    13981 ctgatggcgt tcgtggtact gcctatcgcc tggcagcagg gcatctactt aatccgcgat
    14041 atgccgggga tgctcaataa gctttctgac tttgccgcca cgttgccgcg ccgctatccg
    14101 gcgttaatgg atgcgggcat tattgatgca atggccgaaa atatgcgcag tcggatgctg
    14161 accatgggcg attcggtggt gaaaatttcc ctcgcctcgc tggtcggttt gctgaccatt
    14221 gccgtctatc tggtgctggt accattgatg gtcttcttcc tgctgaaaga caaagagcag
    14281 atgctgaatg ccgttcgccg ggtgctgccg cgtaaccgtg gactggcagg acaggtgtgg
    14341 aaggagatga atcaacaaat caccaactat atccgcggca aagtgctgga gatgatcgta
    14401 gtagggatcg ccacctggct gggattctta ctcttcgggc tgaactattc gctgctgctg
    14461 gcggtgctgg tcggcttctc ggtacttatt ccgtacattg gcgcatttgt ggtgaccatt
    14521 ccggtggttg gcgtggcgct attccagttt ggtgcaggca ctgaattctg gagctgtttc
    14581 gcggtgtatc tgatcattca ggcgcttgac ggcaacctgt tagtaccggt gttgttctcc
    14641 gaagcggtta acctgcatcc gctggtgatt attttatcgg tggtgatctt cggtggtttg
    14701 tggggattct ggggcgtatt cttcgccatt ccattggcga cgctgatcaa agccgtgatc
    14761 cacgcctggc ccgatgggca aatagcgcaa gaataatctt cagcccgacg aactgtttcg
    14821 ccgggctgat ttttcaggat tcctgacgta aataaatagc ccgataacaa atactcacca
    14881 gcactgcacc gccgataata ttacccagca tcaccgggag cagattggcg gtaataaaat
    14941 ggctgaccgt cagtgcagga aaattgtctg cgctactgtg cgccagttgc cagaaggagg
    15001 taggggcgaa atggcgaatg gcaatggcga acggaatcac aaacaaattg gcgatacagt
    15061 gctcaaagcc actggcgaca aacagggtga tgggcaaaat catggcgacg attttgtcgc
    15121 ataacgaacg cccgcaataa ctcatccaca gcgccaggca aaccatcaga ttgcacataa
    15181 tgccgaggct gacagattca gtaaatgtat gatgcatttt gccctcggcg cagtgtaaaa
    15241 ccgcgactcc ccacatcgcg ttttcactca tcaccagccc ggaaaaccag attaacaaac
    15301 tgaaacaggc aatacctgcc agattaccgc aggccaccag aagtgcgtta atcagccaag
    15361 ttcgccaact aataacgccc cgactttttg ccatcaccgt cattaccgac gaggtgaaca
    15421 ggctggtgcc gcaaaccgcc agcaaaataa agccgagtgt aaagcaaagg ccgcccacca
    15481 gatgggttaa tgcctgtgaa gacggggcat cggcgataac ggaaaggtaa aaagtaaatc
    15541 cgatcgccat aaacacgcca gcggacatcg ccattaaaaa gaccgacact ttactgcggg
    15601 caattttatg ggcggcaatc cgttcagcga ttgccgcttt tctggcgctc aactgcaagt
    15661 cgaaagagag tttgtttctc atgcatactc ctgcaaaagc agattacaac acctcgcgaa
    15721 ccgagatccc cagacgctgc attcgtgaca gcagcgtggt gcgcttcatc cctaatcgtg
    15781 tcgccgcgcc acggggcccg gcaacaatgc cattggtttc tcgcaatacc tgaacaatgc
    15841 gctgacgttc ttcttcgtca ttctctggcg tcgtcgggtg cagcaactgc gccattgaac
    15901 tgcgaagcgc tgaatcttca tttagcgtcg gcagtaaacg gctttgtcgg acattaagat
    15961 gtaaattcag actgttaccg cgagtcagca gtaccgctcg ctcaatcacg ttttccagct
    16021 cgcgcacgtt gcccggccaa tcccacgaca tcaactggcg tagtgcctcg gtcgggatgg
    16081 cgtcaattgc gcgattcata tggcgcgcca ttttttgcgt gaaatgtttt gctaaaagag
    16141 ggatatcttc cggacggtct cgcagcggcg gcaattccag tgggaagaca ttcaggcgat
    16201 aaaagagatc gctgcgaaac tggcgatctt caaccatttg ccacaaatca cggttggtgg
    16261 cggcaatgac tctgacattc accgggatcg ttctactccc gccgagacgc tcaatttccc
    16321 gctcctgcaa tacgcgcagc aatttaggct gaagttctaa cggcagatcg ccaatttcat
    16381 cgagaaacaa cgtaccgcca tcggcaattt caaaacgacc acgatgggta ttaatcgcac
    16441 cagtaaaggc ccctttgtca tgaccgaata actcactttc cagaagactg gcggggatgg
    16501 cagcgcagtt gattttgacc agcggcttgt cgcgtcgcgg gctaagttga tggatcgctc
    16561 tggcgatcac ctctttgccg gttccggttt caccacaaat caacaccgta ctgtcgctct
    16621 tcgccacaat atctacctgc tggagcaggt cttccattgc ctggctttga tagataatgt
    16681 caccgacgcc cagattcgaa aggagctgct cgctaagctg gtggttttct tgctgcaaac
    16741 tttcctgcaa atcggtcatg ctacgccagg catcggcatt gccaacggca atagcgatgc
    16801 gatcggctat gtgttgtagt agctggcagt tttcctcact aaagagagtg gtagaggtat
    16861 gcgccagcaa caatgcaccc ggtgtatggt tgccaaaggt aagcggtatc aggagcgaag
    16921 attcgcagcc gttagatgca agtaagtgta ataacgggtc gcgttgccag agaaacagat
    16981 cgtctgcacg gtgcgtcaag gtcggttgtc gggtttgtag cgtttgtgtc agcaatacac
    17041 tgtcgccagg catattgcgt ggcagacacg cacaatgtga ggcagaaaga tcgctgcacc
    17101 acaggctgaa cttctcgttc tttcgatgat cgcccagtac catactgact gaagccagac
    17161 cgaaaaaatg atggatctca cgagcgacgt cagcgatcag atcgtcgaga tcaagatgtg
    17221 acagcactgc attagtgata tccaccagta cccgatgatg atcgcgatct ttctccagca
    17281 aacgcagcgc cacctgttgt tcttcatcat tgagccatga ttgcagaacc ggggtgataa
    17341 tgcgtgccag ccagttaaac agtgtgcaat cctggggagt aaaacgactg ccgtgggtgc
    17401 gaataaagcg tagctccgcc attccactac tgccgagaag gacggcgtga cgcgaataat
    17461 cgaccatctc gccctgatac tctgttgttg ccgtcacatc gctgccatca tcatggtagc
    17521 ggtaatagaa gttgcttttg agaacatgca gttcaataag ttgtaccggg ctgaacggcg
    17581 gcggcagcgg gatgaaggcg cgtaataaag acgccttgcc gtgtttctgc gctaaccgct
    17641 ctgcgagcat tccgtttacc gcttcaattg ttattccttg cggcggggca aacatcgcct
    17701 cgtctgacat agccatcaac agtccttatt aatgagaaat ccttctcacg caatttttct
    17761 ggccatcaga tagatagccg gttccagcac aatttcatcc aacagatgaa tgaattgaat
    17821 gctacgcgct tgccacacag acgtcgcgtc atctgccagt ggcgcgaatg cccgggtcaa
    17881 cagggcggtg tggctggcgt caataacaat ctcaccaaaa gtcatcaccc ccagcatctt
    17941 acgtcgggct tgctcctctt cgaccagtgc aagccaatct tcgtattccg tcagtgggca
    18001 gcggaaggtg acattcagac aatcaatcac gccaacgtga tgaccgatag ccagcgagta
    18061 atacattacc tgggagctgt gttccggcat ctcgtcgcta ctggcgacaa acttttttcg
    18121 cagcgtccag aaaacaattt ccccgcactc ttcagtcatg taacccccgg agtacgcgaa
    18181 agagttgctg aacgatacta ttgagacgtg gatcgtcggc atcgcgcaac caggtgttaa
    18241 cccgatttcc tgtaggatcg ttgcttaaat ggcgcaggag ccgatcgcaa atttcttgcc
    18301 cctgacgata gcccgccagc cgccgcgctt ctcgctcaat ggcgatacgc tgtgatggcg
    18361 ggatctgcgg ccacaacggt tgcatagtca ccccggtggg atcgcgataa tccaccgcgt
    18421 gaatcttctg ttgcagcaaa ccgagcgcca cggcgaaacc gtgaatggtg gcggccggtg
    18481 ttggcgggca gccggggatc caaacatcaa tggggacaat ggtgtcgctg ccgccccaga
    18541 cgctgtagag atcgtggaaa ataccgccgc cgacaccgca cgcgccgtag gaaacacaga
    18601 ttttatgatc gggggcagac tcatacgccc gaagtgcagg catacgcatc gcccgggtga
    18661 ctgcgccagt aaataacaaa atatcggcgt gacgcggtga tgaaacaacc ttaatgccaa
    18721 aacgttctgc gtcgaatacg ggtgtgatgg cggcaaaaat ttcgatttca caggcgttgc
    18781 agccgccgca atcgacgcga taaacgtagg ctgagcgacg gatatcctgt agcaaatgcc
    18841 gcttcatctt ttgcgtttgc tcgtccagcg tgatgggctg gctgacatgt tgtgtaagca
    18901 ctggactcat agctgctcct tagccaccag cggtacatct gtatcgtcgt tgatcagcgt
    18961 cgcgcgttgt ttgcactccg ggcagacgct cgcttgcgcc cgcaacattt cgcggttttg
    19021 tggcgcattt tgttgctgtg ccaacaattc agcagccagt gcgacggttt tttgcggggc
    19081 aaacgggcgt tcgcaacggc tgcaacgttg tagatggaac gtcgcgcggg tatagagatc
    19141 ggctttattg gtgacggtca gttcaaagtt attggtaagc tggatggctc tggtcgggca
    19201 cacttcttca caacgtccgc agtaaataca acgccccaga tagagctgcc aggtgcgcga
    19261 attttgctgg tcgtcggtct ggatagtcag cgcatttgtc ggacaagcac aggcgcaggc
    19321 accacaggca atacattggc tgggcatcag gtccggtttc ccgcgaaagc ctgggctgac
    19381 ctccagtggc gcgaagggat atttcaccgt cgcggttccg gcgcgcataa tagttttcag
    19441 taacttcagc atcttctgtc cttacttcag cggcgaacgg ttacgatcaa tgccgtagcg
    19501 ttcgatctct ttatacggca cggtttttga ctggcgcttg cgcacatcaa ccagcgtcac
    19561 acggtcggta caggagtagc agggatcaag gctaccgata atcagcggtg cgtcagaaac
    19621 ggtattgccg cgcagcatgt aacgcaacac cggccagttg gcgtaggtgg cggcacggca
    19681 gcgccagcgg aacaattttt ggttatcacc gagcatgctc cagtgcacgt cttcaccgcg
    19741 tggcgcttca acaaagccga gcgcgaatgc gtgaggttta tagctaaagc cttcggtcag
    19801 cagtggggta tccggcatgt tgtcgagagc aaattccagc attgccagcg aatcaaacgt
    19861 ctctttgaca cggaccatca cgcgggagaa aacatcgccg ccggtgaagg taaacagtgt
    19921 ttttggaata ttgccgtagt cggcgtaggg gtgatcaaag cgcaaatcac gggcaaaacc
    19981 actgccgcgg atcagcggcc ctacagggct ataatcacgg gcgatttgtc ggtcgagaat
    20041 gccaatgccc tgagtgcgtt gttccatatt cggcgtggca agcagcattt ctaccagctc
    20101 cgacacgtcg gcgcgcatct cgcgcaccag tttcagcgtt tgcagacgtt gctctttgag
    20161 aatatcgcgg cgaacaccac caatcagatt cagaccgtag gttttacgcg acccggtcag
    20221 caattccgcc attgtcatcg acttttcccg cacgcggaaa aattgcataa agccggtatc
    20281 aaatccaaca aaatggcagg agaggccaag gttgagcaaa tgactgtgta gccgttcgac
    20341 ttccagcaga atcgagcgaa tggtatgcgc tcgttgcggc acctcaatcc ccagtgcatt
    20401 ttcaaccgag ttggtatagg ccacactgtg ggcaaaaccg caaatcccac acacgcggtc
    20461 cgataagaag gtcacttcgt tgtagcccat ccgcgtttct gccagtttct ccatgccgcg
    20521 atggacataa aacagacggt aatcagcatc gacaatttgc tcgccatcca caaacaagcg
    20581 gaagtgaccc ggttcatcgg aggtgatatg cagcgggccg acagggatca cccgcgcacc
    20641 gctgttgccc tcattgataa acggatacgt ttcggaatca gtcgtcggtt cagggcgcag
    20701 tcgataatcc atcgcatctt tgcgcagcgg atgcatatct tccggccagt catcgggcaa
    20761 caccaggcga cgctgatccg gcaggccaac cggaatcagc ccgtacatat cacgaatttc
    20821 tcgctcgccc cagaccgcgg cagggacgcg cggtgtgact gacggaaact cccgactgtc
    20881 ggcatcgacc agcgccttca ccacaatcca gcatttttcg gccccttcca ttgaaagggc
    20941 ataataaacc gcgtaatgac cgttaagtgt ccgctcgtcg ttgccaaaca ggaccggaag
    21001 ccagccatca tgttgataat aaagatactg tacaacgtca ggcagcagat tgattttcac
    21061 cgtaatggtg acctgttcag gcgtttgtcg ctcttcatcc agcaccgcgc cggggaactg
    21121 cgttttcagg gcggcgagaa tcgcttcgcc acgatttgac gatgaattaa cgttcactgt
    21181 gtttctccga aacagatgcg gtgcctgaag gcaaaaaatc atgccaggtg ctacgttgtg
    21241 caggcaggtc gtgcgtccct gagaggacta tagtggaagc gcccgccagg atcctgatga
    21301 caggttgtgg aatatgcgtt cccatcgcca gcatcatgac cagcagaatc accattggcg
    21361 aggtggtcaa ccagccgaga tcaccccggt taacggcctg cggcggtttc gccattaaca
    21421 cccgcgcagc catccgtacc aggcccgcca gcaccagcgt taacagcaat aacagcagga
    21481 cgataatcag caggtgatta cgtgccagtc cggcggtaac ggtcataaat tcgctaagaa
    21541 aaatgttgaa gggcggcatc cctgccagcg ccagcgcacc gccgccaaac agcacggcgg
    21601 taaatggcat gattttgagc atcccacaga cgacgttgag atcgcgcgtg ccgtacttga
    21661 gcagtacatt gccggaaccg cagaacagca gcgtttttgc cagactgtgg tttaaggtgt
    21721 gcagcagcgc ggcaaaaatt cccagcggcc cgccaatgcc tagcgccacc gcgaccagtc
    21781 ccatgttctc cacgctggag tacgccagca gacgcttaat gtcccgctgt accagaatga
    21841 aaaatgccgc cacggcaacc gacaacatgc cgaagatgag caacaaccgg ttggggaaat
    21901 cgctgccgat ggcctggcaa ataatgatgt aatagcgaat cagcaccaac agcgcgcagt
    21961 tcagcaatac ggcggagagt agggcgctga ccggactcgg cgcttcactg tgagcatccg
    22021 gcagccaggc gtgcatggga aatagcccgg ttttggtacc aaagccaatt agcacaaaca
    22081 caaaggccaa cagcattaat gttggatcaa gcaaggacga ttgcttaaga acctcgctcc
    22141 agaatatcgc catttctgcc tgcggcataa cgctggcggc gttggcgtat actagcacgg
    22201 taccgaacag accaaaagcg acaccaacag tacaaataat gatgtacttc catgctgctt
    22261 ccagcgatga acgctgaccg taaatgccta ccagaaacgc cgagcttaag gtggtggctt
    22321 cgatcgccgc ccacatcaca atcaggttat tgctggtaac aaccagcagc atggtgaaca
    22381 aaaacagatg gaagaaaccg tagtaatcgc acagcgtaac gggtgaaagc tcgccgtgct
    22441 caacttcatg acgcatgtag ccaatcgagt aaacaccggt gagaaagcca atcacaccaa
    22501 gaatggcgag gaacaaaccg cccagaccat caatatgaag ccacagtccc gcagcgaata
    22561 tttctcctgc atcagcggca gtttggacca cccagagtgc cagaatcagc agcagtgtga
    22621 tccctaagct atgtaatact gtcaccgtgc gagtcgcaga gtgccctcgt ttccggcagg
    22681 caaaacagag cagcgaaaaa agcagcggag tgagcaggag taaagcgaac atcacagaat
    22741 aactcatctc attatccctt cagcgcggtc aagttgttca cgtccagcgt gccgtgggta
    22801 cgccatattt ttcttgccag taacaccatc acaatgacgg cgaagatggc gtcggtagcg
    22861 ataccaattt ccaccagttc cggtgcacgc caggctagaa gcgccagcac cagatgggag
    22921 ccgttttcca tcaggcagta accaaaaatt tgccgcagga tattgcgctg gctaacaatg
    22981 cacagcaggc caagcagaaa atgacctaac gccaccgcca gcgctggttt cagcccggta
    23041 gccatcgata gcttcacggg ctgaacgaca aatgcgcaaa gcaggacaat taacgccgcg
    23101 agcagtgcca tcattgccgg accgaataac gctttttccg ggatgttctg gggaatattt
    23161 cgtgcagcgt aagtcatgat taacggtacc agcagcactt tggtgataaa ggcgctggcg
    23221 gaccagatca gcagttgctc tgcggcgaac aggcacgaga gggtggcgaa aatagacacc
    23281 agcaccagcg actggcaggc gtaaaatccg catgacaggc gatagctttt gacgctaatc
    23341 acaaacagcg atgtcagcat catcagtccc gccagattat ttacgatcat agaaccggtc
    23401 attcatcatc tccttactgc aaccaggtcg cggcgattac gctggaaatc agcgacatca
    23461 caatcagtac aatcagcacc aggcgcattg accctggcag cggtgcggca tcggcgacgg
    23521 cctcgctcgg tttgccgggg acgacgcgac caaaccagcg aataaaccag gcgaaactgg
    23581 cgaccgattc aatcatcagc agaatcatgg cgggcagcag gatccagtac tccactgaca
    23641 acgcaaaacc ggcagcaaac agcgggaatt tactgaagaa gccgttgaac ggcggtacgc
    23701 cggtaatcgc cagcgctgcc acgcagaaac ccacgcctgg caacggcagg gtgtgcaata
    23761 cgccacgcag acgcggcaac aagcgcgtgc cgcagctgta actcagcgca cctgctacaa
    23821 ggaaaaacag gcttttagcg aacgcgtggt tgacgatgta ggcgatgctg ccctccagcg
    23881 ccagccgcga gccgaagatg gagagcgaca agccgaagaa catccagcca agttgagtga
    23941 tggtcgacca ggccagcaac cgcttcatat cctgctgtgg caaatacatc agaaatccat
    24001 aaagaatggt gaccagggcc atgaccatgc caacgccgcc aatcacatgc gggatattgc
    24061 cgccgtcgat aatggcgcgg gcaaaaatgt aaacgcccac tttcaccatc gatgcggcgt
    24121 ggagataggc gctgatcggt gttggcgctt ccattgcgtc cggtagccac gcttgcatcg
    24181 gtagctgggc cgatttcccc cacgcggcaa acaggatgcc gccataaacc agataacgtg
    24241 cgtcgccgtg taactcgctc atcgcgctaa gcgcaaacgt tccggtctgc aaaaacagcg
    24301 tggcggcggc aagatacaac cccaacgaac cgatatgggt gataagcaac gcttttagtg
    24361 ctgaacgctg cgctttatcg ctctgataat aactgatcaa cacccaggag cagccgcccg
    24421 taatttcaaa aaacaacaac tgaccgagca gcgtcgagga gagtaccagt cccgccatcg
    24481 cgccgataaa caccagcaaa aatgcgtaat aacgattcgt gccgttatgc gggtgttcgc
    24541 gatttttatc cgtcagataa cccgtggagt agatcgtgac cagcaaaccg agaaacacca
    24601 ccacaaacag aatcagcgta cacacgcgat caatgaccag gccaaacaat gccacatccc
    24661 ccacgttgac caacgtcaac gtgacggcaa ctttatcggc ctgataaaac gcggagatca
    24721 gcgacaacat gcacagcgtg gtcagcgcgg cgaacaaaac cccccattcg gcggcccgac
    24781 gttgtggcga aaacgaaacg accagtgcgc cgataaaagg cagcaataac gtcgtcagag
    24841 caagattttc catattccgt cagtgctcct tacagaccgg ttaaccagaa gacccatgca
    24901 agcgcagcaa ggctgaagcc aagccaggtc acatggtgaa tgagtaaaaa acgcccgcgt
    24961 gccagcgtgt tttctgcgat tgaggccagt acaaaaatca gcaaaacctt gagcagcgta
    25021 acgacaagtg aagtcagcag gcaggcgaga gaaagttctt gcgcgcaccc aaagggcaga
    25081 aacagggcca caaacagtgc tgccatcacg acctgtttca gccccagccc ccatttcgcc
    25141 agcgccagcc cggcaccgga atattctgtc agcgggcctt cctgtaattc ctgttctgct
    25201 tcagcaacat caaagggaat ttttcccatc tcaatgaagc aggcaaaacc acaggccagt
    25261 aacgccagta cggtggttag cggcgagttc cagcccgttg ccagcgtatt gctgatcatc
    25321 tcgatatgcg tggaacctgc tatcagcgcc aataccagca gtgagagaat gagcattggt
    25381 tcgaccagaa tgccgagcgt caactcgcga ctggcaccga ctcccgcaaa cgggcttccg
    25441 gtatccagcc cggaaagagc aaagaaaaaa cgaaacaggg caagaagata gataagggtg
    25501 atcagatcgc cgccgcccgc aaaaggggaa acggtaataa atagtggtag ggccatcgcc
    25561 agcaccagca tgctgctgat taatacccac ggcatcaggc ggaacatcag acctgaagat
    25621 ataggcgcaa cttcctggcg tttaaacagt ttgtggatat cgcgataatc ctgccagatc
    25681 cccggcccgc ggcgggagtg catacgcgcg cgtatctgcc gggaaatacc cgtaaaaagc
    25741 ggggttagca tcagcagaat cacggcctgt gctaacgcaa aaatgaccag atatccgtcg
    25801 caaagagttt gtctcatggt gatttcctta gacggcaata gcgattagca gcacaaccag
    25861 tgcggcgacc acatacaggc aatagagacg aaagtcgccg ctttgcagat gctgtatttc
    25921 tttggccagc cgttgggtgg cgctcacgat ggggcggatc acccgctcat cccagaaggg
    25981 ttctgtgctc tgagccctgg cggtgacgtg cgcaagacct ttatttagcc tcagcgtagg
    26041 gtcgagctgt tgtcgtagac gaaatagcgc agagaagacc acacgcagcg gctgcatcac
    26101 gccattgcct gacggggcca tcgcattttc ccagccgtag ccgcatgccc aggcatctcc
    26161 tgtgcgacga aacgctgcgc ggcgcgaacg acaccacagc cagtaaaggc caggcagcaa
    26221 aggcatcgcc agtagtaaca gcaacagtaa tgagggggtg acctgtgtat gaaacgtgcc
    26281 gggtacaagt gctattccgc tggcgacagt gacgggaggg gtattggtaa acgcatgggc
    26341 aatatgcatt atcttcggtg ccagccaact ggcactaagc gcaattaata cgcagagtgc
    26401 cgcgagcagt agcatggcga cgatcatcgt atttggcact tcctgtgcct cttcagcgtg
    26461 tgtactgcgc ggcgcaccac agaaagtaat accgtacatt ttgacgaagc acattactgc
    26521 cagcccacca gtgactgcca gcatcaccat agcaatagga cccgcaagtt gtagcgctac
    26581 ggcttccaca cgacttagtg agaacagcga ctgccaggtg taccattcgc tgataaaacc
    26641 attcagcgga ggaagggctg atatcgcgag gcaaccaatc aggcatgctg cggctgtcca
    26701 cggcatccgt ttcgccagtg cccccatttt ttccatgtcg tgggtatgca aacgcgaaat
    26761 aatcgcgccc gcgccgagaa atagcagccc tttgaacagc gcatggttga gcagatgaaa
    26821 cagtgcgccg agcagtccaa ccacggtgag cagcgggtcg tgtagtgaca gaccgaccat
    26881 cgccacaccg actgccagca aaataatgcc gacgttttcg acggtactcc aggccagcag
    26941 ccgtttgata tcctgttccg ccagcgcata cagcacgccc aggagcgcgg agattgcgcc
    27001 gatcgccatc accagaatgc cccaccacag cggcaaaccc gtttgcgcca gcagatccat
    27061 cgccactttc aggatgccga aaataccgat tttgaccatt acgccagaca tcaacgccga
    27121 agcgtgcgat ggtgcggcag ggtgagcgcg cggcaaccag ctgtgcaacg gcatcatccc
    27181 ggctttcgcg ccaaaaccga aaaaggccag caggaacacc gccgacgcca gccccggaga
    27241 aagtgaaagc gtgcggaaac tggcaaaatc taggctgccg ctttcgcgcc ccatcagcaa
    27301 gaaggcgatc ataatcagca ccgatccggc gtgggcgata aaaaagtaga gcatgccagc
    27361 gttgatcgac gttttatcct gcctggcaat gaccagaaac caggaagaca gcgacatcat
    27421 ttcaaacagc acgatgaacc aaaaagcgtt gtccatcacc agcagggcaa ccatcgatgc
    27481 gatgaaaata ttcataaaga agcccatcgc cgccgcgcct ttgccctcgt attcgcgcat
    27541 ataagtcaat gaatagagcg aacaaaccac caccagcaag gagataacca gcaccataaa
    27601 tgccgagagg ctatccattc gcagggttaa atcggcaaag tcaaaagggg tggcaaaacg
    27661 ggcaactaaa gttacgcctt catgtaattg cgtaattccg ctgatgatgc caatcacgcc
    27721 gcccaccagc gatgtgatgc cggaaagctt aatagccagt ctgtccagac cgagtaaaaa
    27781 cagcgaagcc agactggcaa acagatagag gatcagcgac caggttaata attgcagggc
    27841 atccattagc gttgctcctg agtgagggaa aggggatcct ccctgccatt ggcaaaccag
    27901 cttgcggcaa ggcgctgttt ttctttcatc tgacgttgca ggctatcgtc ggtgatcagc
    27961 cgtaacgcct gattcgggca agcgcgaacg caggcaggac cttctggcaa gaaatcacac
    28021 aggtcgcatt tcaccgcgac ggtctggaca cccggttccc agcgcagcaa agcatgttgt
    28081 gttggcgcgt tttcttcgcc gtctttgagt gagccttcag cctgaaaaac atattgcgca
    28141 ggggcattca ccggacggct tcctgaagcg ctgattgcgc caaatgggca gaccacggcg
    28201 caaagcttgc agccaataca gaggctttcg ttgagttgga tcgcatcatc cctctgggag
    28261 atggcattga ccgggcagac ctgcacgcaa ggggcttcct cacagtgatg acacacgaca
    28321 ggggcagtga gtgttgacgt cttcgccagg gccaggcgcg ggtgttgctg taaaccttgc
    28381 gttttatgca cgtccgaaca ggcagcgaga caggtattac atcctgtaca ccacagtggt
    28441 tcggccacca caaagcggtt catgtcggct ccttgaaaat gatatgaaac attgcatttc
    28501 ataatgcata aaggatgcca gtatgattaa tttaatatat tcaataagtt atgaattaac
    28561 tgaattctga acggtacaaa aatgatggtc attaacgttc agcaaaaaat ggattcgaca
    28621 cccgtgtcgc aggggaaagt gagaatgtga tcttgatcac gataagtgag gtgtgctgac
    28681 cgcaagcgca gccagcacgg aatggagcaa agtaatcagg cgtgttcttt cagccagttc
    28741 agcacaacgt cgtggtgatt gctggttttg aaatcgtcaa agacatgttc gattttgcca
    28801 tcagcgtcaa tcaggaagct gatgcgatga ataccatcgt aggttttgcc catgaaggac
    28861 ttttcccccc agacgccgaa ttgctcgcac acctggtggt cctcatcaga caggagcgta
    28921 aagttaagca gctctttttc cgcaaaacgg gagagttttt cgggtttatc ggtgctgata
    28981 cccagcacat caacgcccgc ttttttcaac tcatccatgt tatcgcgtaa gccgcaggcc
    29041 tgtacggtac agccgggggt catggctttc gggtagaaat aaaccagaac acgctgtccc
    29101 tggaagtcgg tcaaattaac ttgttctccg tcttgatccg gcaagctaaa tttcggtgcg
    29161 atatcaccgg ctttcagtgg attcattact taactccatc ctgttcatca tgttgggaat
    29221 aattgacgac gttaatactg ccttgtgcat tgagttctgt acatagggct ttgaacgctt
    29281 gctcaatatt tgctgcgtcc gcagatgcgg ggctgtgggc agtaatctga atatgcaact
    29341 gcgcagcccg ttcattttca gcaggttgtg tgcgcgacac cagctccgca atgttcatat
    29401 gatgcgcgtc gaaaagtgct gtgaagcgtt caattaaatg cggggagtct gccacatcga
    29461 cctgaaccca gacagatgct ggcattggcg gacgcggacg cgccgtcgtg cgcttcatca
    29521 cgattaaaag atccagttcg gcacctttca acggtaacgt tgattcaatc agagtaatgg
    29581 cattccatga accggaaagc agcatgataa acgtgaactc ttctcccagc atcgccaggc
    29641 gactgtcttc aatattacag ccgcaactac tgacatgacg ggtgatggtg ttcacaattc
    29701 cagggcgatc ggcacccaac gcagtgatca ccagataatg ttgcgatgac agtgtcaaac
    29761 tggttattcc tttaaggggt gagttgttct taaggaaagc ataaaaaaaa catgcataca
    29821 acaatcagaa cggttctgtc tgcttgcttt taatgccata ccaaacgtac cattgagaca
    29881 cttgtttgca cagaggatgg cccatgttca cgggaagtat tgtcgcgatt gttactccga
    29941 tggatgaaaa aggtaatgtc tgtcgggcta gcttgaaaaa actgattgat tatcatgtcg
    30001 ccagcggtac ttcggcgatc gtttctgttg gcaccactgg cgagtccgct accttaaatc
    30061 atgacgaaca tgctgatgtg gtgatgatga cgctggagct ggctgacggg cgcattccgg
    30121 tgattgccgg gaccggtgct aacgctactg cggaagccat tagcctgacg cagcgcttca
    30181 atgacagtgg tatcgtcggc tgcctgacgg taacccctta ctacaatcgt ccgtcgcaag
    30241 aaggtttgta tcagcatttc aaagccatcg ctgagcatac tgacctgccg caaattctgt
    30301 ataatgtgcc gtcccgtact ggctgcgatc tgctcccgga aacggtgggc cgtctggcga
    30361 aagtaaaaaa tattatcgga atcaaagagg caacagggaa cttaacgcgt gtaaaccaga
    30421 tcaaagagct ggtttcagat gattttgttc tgctgagcgg cgatgatgcg agcgcgctgg
    30481 acttcatgca attaggcggt catggggtta tttccgttac ggctaacgtc gcagcgcgtg
    30541 atatggccca gatgtgcaaa ctggcagcag aagggcattt tgccgaggca cgcgttatta
    30601 atcagcgtct gatgccatta cacaacaaac tatttgtcga acccaatcca atcccggtga
    30661 aatgggcatg taaggaactg ggtcttgtgg cgaccgatac gctgcgcctg ccaatgacac
    30721 caatcaccga cagtggtcgt gagacggtca gagcggcgct taagcatgcc ggtttgctgt
    30781 aaagtttagg gagatttgat ggcttactct gttcataagt cgcgcctggc aacggttgcg
    30841 ggtgtttcgc ttgttttatt actcgctgcc tgtagttctg actcacgcta taagcgtcag
    30901 gtcagtggtg atgaagccta cctggaagcg gcaccgcttg cggagcttca tgccccggct
    30961 ggaatgattt tgccggtgac ctccggtgat tatgcaatcc cggtgaccaa cggtagtggt
    31021 gctgtcggta aggcgctgga cattcgtcca ccagcccagc cgctggcact ggtttctggc
    31081 gcgcgtaccc agttcacggg cgataccgct tcattgctgg tggaaaatgg tcgtggcaat
    31141 actctgtggc cgcaggtggt tagcgtgctg caggcgaaaa actacaccat cacccaacgt
    31201 gatgatgctg gtcagacact gaccaccgat tgggtacaat ggaaccgtct ggacgaagac
    31261 gagcagtatc gtggtcgtta tcaaatctct gttaagccgc agggttatca gcaggcggtt
    31321 acggttaaac tgctgaacct ggaacaggcg ggcaaaccgg ttgcagacgc ggcttccatg
    31381 cagcgttaca gcacggagat gatgaacgtt atttccgctg gtctggataa atctgccact
    31441 gacgccgcga acgctgcgca aaatcgtgcc tccaccacta tggacgtaca aagtgcagct
    31501 gatgacaccg gtttaccaat gctggtcgta cgcgggccgt tcaatgtggt ttggcaacgt
    31561 ctgccagcgg cgctggaaaa agtgggcatg aaagtgaccg acagcacccg ttcgcagggc
    31621 aacatggccg taacttataa gccgctgtct gacagcgact ggcaggaact gggcgcgagc
    31681 gatccaggcc tggcatctgg tgactataaa ctgcaggttg gcgatttaga taaccgcagc
    31741 agcctacagt tcatcgatcc gaaaggtcat actctgactc agagtcagaa cgacgcgctg
    31801 gtagctgtct tccaggctgc gtttagcaag taaaaataca gggctggaat catccggccc
    31861 ttttttctga tatgatacgc aaacgtgtgc gtcggcagga aaacgcgatt ttagcggtaa
    31921 ttcgcacgaa atttgtttgt cggacgtagt tcggataagg cgttcacgcc gcatccgaca
    31981 aaacatccgg cacaccagac agcaaaagat tttaaaacgt taattcacac ccaggagtga
    32041 taaagatgca aaagcaagct gagttgtatc gtggtaaagc gaaaaccgta tacagcacgg
    32101 aaaacccgga cctgttggtg ctcgaattcc gcaatgatac gtcagcaggg gatggcgcgc
    32161 gcattgagca gtttgatcgc aaaggtatgg tgaacaacaa gttcaactac ttcattatga
    32221 gcaaactggc tgaagcgggt atcccgactc aaatggagcg tctgctctcc gataccgaat
    32281 gtctggtgaa aaagctggat atggtgccgg ttgagtgtgt cgtgcgtaac cgtgctgctg
    32341 gctctctggt gaaacgtctt ggaatcgaag aaggtattga gctgaacccg ccgctgttcg
    32401 atctgttcct gaaaaacgac gccatgcacg atccgatggt caacgaatct tactgcgaaa
    32461 cctttggctg ggtaagcaaa gagaacctgg cacgcatgaa agagctgacc tacaaagcga
    32521 acgacgtgct gaaaaaactg ttcgatgatg ctggtctgat tctggtcgac ttcaagctgg
    32581 agtttggtct gtacaaaggt gaagtggtac tgggcgatga gttctccccg gacggtagcc
    32641 gcctgtggga caaagaaacg ctggagaaaa tggacaaaga ccgtttccgc cagagcctcg
    32701 gtggcctgat cgaggcctat gaagccgtcg cccgccgcct gggtgtacag ctggactgat
    32761 ttttctgttc atcatcttgc cgtgctgctg gcacggcaag acaaccattc tcgtaagatg
    32821 tgcattgaac gctcattcct ccgccatttc atcccgtttt cctgtggtaa tcttcctctg
    32881 aaccacctac gatcatttct ataatgaata tattgttgag gtatctatgc gttggcaagg
    32941 gcgacgtgaa agtgacaatg ttgaagacag gcgcaatagc tccggcggtc cgtcaatggg
    33001 cggtcccggt ttccgtctgc cgagcggtaa aggcgggctg attttactga tagtcgtgct
    33061 ggttgcaggc tactatggcg ttgatttaac cgggttgatg accgggcagc cggtttctca
    33121 acagcaatca acgcggtcaa ttagcccaaa tgaagacgaa gccgcaaaat tcacctcggt
    33181 gattctggca accacggaag acacctgggg acaacagttc gagaagatgg gtaagaccta
    33241 tcagcaaccg aagctggtca tgtaccgtgg aatgacgcgt accggatgcg gggcgggcca
    33301 gtccataatg gggccgttct attgcccggc ggatggtacg gtttatatcg atctctcctt
    33361 ctatgatgac atgaaagaca aacttggcgc ggatggcgat tttgcccagg ggtacgttat
    33421 cgcccatgaa gtcggtcatc atgtgcagaa actgttaggc atcgagccga aagttcgtca
    33481 actgcaacaa aacgcgacgc aggcggaagt gaaccgctta tctgtgcgta tggaactcca
    33541 ggccgactgt tttgccggtg tctgggggca tagcatgcag cagcaaggcg ttctggaaac
    33601 cggcgatctg gaagaggcgc tgaacgcggc gcaggccatc ggcgatgacc gtttacaaca
    33661 gcaaagtcag gggcgagtag taccagacag tttcactcat ggcacttctc agcaacgcta
    33721 cagctggttt aaacgtggtt tcgacagcgg cgatccggca caatgcaata cttttggtaa
    33781 aagcatttaa cttccggggc agggatggct gaactgactg cgcttcacac attaacagcg
    33841 caaatgaaac gtgaagggat ccgccgcttg ctggtgttga gcggggaaga gggttggtgt
    33901 tttgatcatg cgcttaagtt acgtgatgcc ttacctggcg actggctgtg gatttcgccg
    33961 cagccagatg ctgaaaacca ctgttctccc tcggcactac aaactttact tgggcgcgag
    34021 ttccggcatg cggtattcga cgcccgccac ggctttgatg ccgctgcctt tgccgcactt
    34081 agcggaacgt tgaaagcggg aagctggctg gttttgttac tccctgtatg ggaagagtgg
    34141 gaaaaccaac ctgatgccga ctcgctgcgc tggagtgatt gccctgaccc tattgcgacg
    34201 ccgcattttg tccagcattt caaacgcgta cttacggcgg ataacgacgc tatcttctgg
    34261 cggcaaaacc agccgttctc gttggcgcat tttactcccc gtactgactg gcaccccgcg
    34321 accggcgcac cacaaccaga acaacagcaa atcttacagc agctactgac catgccgtcg
    34381 ggcgtggcag cggtaactgc tgcgcgtggg cgcggtaaat cggcgctggc agggcaactc
    34441 atttctcgta ttgcgggtag tgcgattatc actgcgcccg caaaagcggc aacggatgta
    34501 ctggcacaat ttgcgggcga gaagtttcgc tttattgcgc ctgatgcctt gttagccagc
    34561 gatgagcaag ccgactggct ggtggtcgat gaagccgcag ccatacctgc gccgttgttg
    34621 catcaactgg tatcgcgttt tcctcgaacg ttgttaacca ctacggtgca gggctacgaa
    34681 ggtaccggac gtggtttttt gctgaaattt tgcgctcgct ttccgcattt acaccgtttt
    34741 gaactgcaac agccgatccg ctgggcacag ggatgcccgc tggaaaaaat ggttagtgag
    34801 gcactggttt ttaacgatga aaacttcacc catacaccac aaggcaatat cgtcatttcc
    34861 gcatttgaac aaacgttatg gcgaagcgag ccagaaacgc cgttaaaggt ttatcagtta
    34921 ttgtctggtg cgcactaccg gacttcgccg ctggatttac gccgcatgat ggatgcacca
    34981 gggcaacatt ttttacaggc ggctggcgaa aacgagattg ccggagcgct gtggctggtg
    35041 gatgaggggg gattatctca agaactcagt caggcggtat gggcaggttt tcgtcgcccg
    35101 cggggtaatc tggtggccca gtcgctggcg gcgcacggca gcaatccact ggcggcgaca
    35161 ttgcgtggac ggcgggtcag ccggatagca gttcatccgg cgcgtcagcg cgaaggcgtt
    35221 gggcaacagc tcattgccag cgctttgcaa tataggcctg gcctcgacta tctttcggtg
    35281 agttttggtt acaccgggga gttatggcgt ttctggcaac gctgcggttt tgtgctggtg
    35341 cgaatgggta atcatcgtga agccagcagc ggttgctata cggcgatggc gctgttaccg
    35401 atgagtgatg cgggtaaaca gctggctgaa cgtgagcatt accgtttacg tcgcgatgcg
    35461 caagctctcg cgcagtggaa tggcgaaaca ctccctgttg atccactaaa caatgccgtc
    35521 ctttctgacg acgactggct tgaactggcc ggttttgctt tcgctcatcg tccgctatta
    35581 acatcgttag gttgcttatt gcgtctgcta caaaccagtg aactggcatt accggcgctg
    35641 cgtgggcgtt tacagaaaaa cgtcagcgac gcgcagttat gtaccacact taaactttca
    35701 ggccgcaaga tgttactggt ccgtcagcgg gaagaggccg cacaggcgct gttcgcactt
    35761 aatgatgttc gcactgagcg tctgcgcgat cgcataacgc aatggcaatt ttttcactga
    35821 ctccttcaat tttctcccat ggtcattgtg agtcagcggc gtaaaattaa tgacatcagt
    35881 taaggagttc gccatgaaac atgaccattt tgttgttcaa agcccggata aacctgcgca
    35941 acagttgtta ctgcttttcc acggtgtcgg ggataacccg gtggcaatgg gggaaatagg
    36001 tagctggttt gcaccgttgt tcccggatgc gctggtggtg agtgtcggtg gcgcggagcc
    36061 gagcggtaat ccggcggggc gtcagtggtt ttcggtgcag ggtattacgg aagataatcg
    36121 ccaggcgcgt gtggatgcca tcatgccgac gtttattgaa acggtgcgct actggcagaa
    36181 acagagcgga gtaggggcta acgccacggc gctaatcggt ttctctcaag gcgcgattat
    36241 ggcgctggag agcattaaag ccgaaccggg ccttgcttca cgcgtcattg cttttaacgg
    36301 acgctatgcg agcctgccgg aaaccgcgtc tacagccacg acaattcact tgattcatgg
    36361 cggtgaagat ccggttatcg atctggcgca tgcagttgcg gcgcaggaag cgttaatcag
    36421 tgccggtggt gacgtgacgc tggatatcgt ggaggattta ggccatgcga tcgacaatcg
    36481 cagtatgcaa tttgccctgg atcacttgcg ttataccatt ccgaaacatt atttcgacga
    36541 agcgctaagc ggcggtaagc ctggcgatga tgatgtgatt gaaatgatgt aataatagcc
    36601 gggtaggcct gataagaccg tatcaggcat catggccgga tgcggcgtta acgccttatc
    36661 cggccactta gcttacttct tcggttgatc ctttttcggc cagtcgtcgt catcgtccca
    36721 cttatcgttg aaatcacgat gcgggggaag ctccggcttg ttggcgagaa atttcttatg
    36781 gtcgacgcgc ttgagatctt taatcacgtt cagcaggacg cctaccaaaa agacaatcac
    36841 cagaatccac caatatttag ccagccagtc catgcttatt tcctcttgca gaaccactca
    36901 tcacgcgacg agctgttcca tgatacgttg atacatacgg gcaagtagct gcaggtcggc
    36961 agcgttcaca cattcattaa ttttatgaat agtggcattg accggcccga gttccaccac
    37021 ctgcgccccc atgcgggcaa taaagcgccc gtcggacgtt ccgcctgtgg tcagtagctg
    37081 cggtttaatt tcattatagt gctcaaccgc gttaacgacc gcatccacca gtttaccgcg
    37141 cgcggtcaaa aatggctgcc cggaaagcca ccaatccacc gtatagcgca gttgatgttt
    37201 ttcaagcagg gcaagcacct gcgctttgat catctcatca gtcagttcgg tgctgaagcg
    37261 gaagttaaac tgcacaaaca gttcacccgg aataacgttg ttactgcccg ttcccgcctg
    37321 aatattggca atctgcatac tggtcgccgg gaagaattca ttgccctgat cccactcaat
    37381 agccactaat tcattaagga aaggtgctgc gcgatgtacc ggattgtcag ccagatgtgg
    37441 gtaggcaaca tgcccctgaa cgccatgaat ggttaggttg caggttaacg atccacgacg
    37501 accatttttc accacatcac ctaccacttc gatactcgac ggttcgccga ccaggcagta
    37561 atcgagacgc tcattacgtg ccattaacgc ttcgacggct tttaccgtac cattgtgggc
    37621 actggcttct tcatcagagg tgatcagaaa tgccagtcgc cctgtatggt tgggatgttg
    37681 tgcgacaaaa cgttctgcag ctaccaccat cgccgccagc gagcctttca tatctgccgc
    37741 accgcgcccg aataacatgc cgtcacgaat ggttggttca aacggcggat tgatccaacg
    37801 atcggcgtcg ccaggcggca ccacgtcggt atgcccggca aaggccaatg tttcaccttg
    37861 cccacgccat gcccaaaaat tctgcgtatc ggcaaagtcc atgcgttcaa cggtaaaacc
    37921 gatcgcctgc aaacgttcaa tcaacaaagc ctggcatcct gcgtcatcag gactcaggga
    37981 agggcggcga ataagctgtt gtgtcagctc aataaccggg cacgacatag actacacctc
    38041 atggaaaaat ttctgataac tggaatcact gaaacccagc agcataggct taccaggcgc
    38101 gcagagcaat ggacgtttga taattgcagg catttcagtc attaatgccg ccgcagaggc
    38161 cgcatcggtg attatattgc gggtggtttc gtccagttta cgccaggttg taccacgggt
    38221 gttgagtaac gcttcccagc ctaattcgtt gataaaaccg ttcaataatt cgctgtccag
    38281 cccatcgacg cggtaatcat gaaaacgata gtcgatgtta ttggcttcca gccaacggcg
    38341 agccttttta atggtgtcac aatttttgat gccgtaaagt gtaaccataa tgaatccttt
    38401 ttcgatttat gagaataaat actcatttaa gagtgaaaac attttgcc
//
LOCUS       DABSYT020000037        38043 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.037, whole genome shotgun sequence.
ACCESSION   DABSYT020000037 DABSYT020000000
VERSION     DABSYT020000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.037"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            108..719
                     /gene="ruvA"
                     /locus_tag="ID725_RS11390"
                     /old_locus_tag="pgaptmp_002278"
     CDS             108..719
                     /gene="ruvA"
                     /locus_tag="ID725_RS11390"
                     /old_locus_tag="pgaptmp_002278"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGV7850870.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            728..1738
                     /gene="ruvB"
                     /locus_tag="ID725_RS11395"
                     /old_locus_tag="pgaptmp_002279"
     CDS             728..1738
                     /gene="ruvB"
                     /locus_tag="ID725_RS11395"
                     /old_locus_tag="pgaptmp_002279"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGV7850871.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1984..2769)
                     /gene="znuB"
                     /locus_tag="ID725_RS11400"
                     /old_locus_tag="pgaptmp_002280"
     CDS             complement(1984..2769)
                     /gene="znuB"
                     /locus_tag="ID725_RS11400"
                     /old_locus_tag="pgaptmp_002280"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGV7850872.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2766..3521)
                     /gene="znuC"
                     /locus_tag="ID725_RS11405"
                     /old_locus_tag="pgaptmp_002281"
     CDS             complement(2766..3521)
                     /gene="znuC"
                     /locus_tag="ID725_RS11405"
                     /old_locus_tag="pgaptmp_002281"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGV7850873.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3600..4532
                     /gene="znuA"
                     /locus_tag="ID725_RS11410"
                     /old_locus_tag="pgaptmp_002282"
     CDS             3600..4532
                     /gene="znuA"
                     /locus_tag="ID725_RS11410"
                     /old_locus_tag="pgaptmp_002282"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGV7850874.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4548..5870
                     /gene="mepM"
                     /locus_tag="ID725_RS11415"
                     /old_locus_tag="pgaptmp_002283"
     CDS             4548..5870
                     /gene="mepM"
                     /locus_tag="ID725_RS11415"
                     /old_locus_tag="pgaptmp_002283"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGV7850875.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5990..6961
                     /gene="lpxM"
                     /locus_tag="ID725_RS11420"
                     /old_locus_tag="pgaptmp_002284"
                     /gene_synonym="msbB"
     CDS             5990..6961
                     /gene="lpxM"
                     /locus_tag="ID725_RS11420"
                     /old_locus_tag="pgaptmp_002284"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGV7850876.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7092..8534)
                     /gene="pyk"
                     /locus_tag="ID725_RS11425"
                     /old_locus_tag="pgaptmp_002285"
     CDS             complement(7092..8534)
                     /gene="pyk"
                     /locus_tag="ID725_RS11425"
                     /old_locus_tag="pgaptmp_002285"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGV7850877.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8662..9531)
                     /gene="hexR"
                     /locus_tag="ID725_RS11430"
                     /old_locus_tag="pgaptmp_002286"
                     /gene_synonym="yebK"
     CDS             complement(8662..9531)
                     /gene="hexR"
                     /locus_tag="ID725_RS11430"
                     /old_locus_tag="pgaptmp_002286"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGV7850878.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9869..11344
                     /gene="zwf"
                     /locus_tag="ID725_RS11435"
                     /old_locus_tag="pgaptmp_002287"
     CDS             9869..11344
                     /gene="zwf"
                     /locus_tag="ID725_RS11435"
                     /old_locus_tag="pgaptmp_002287"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGV7850879.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11579..13390
                     /gene="edd"
                     /locus_tag="ID725_RS11440"
                     /old_locus_tag="pgaptmp_002288"
     CDS             11579..13390
                     /gene="edd"
                     /locus_tag="ID725_RS11440"
                     /old_locus_tag="pgaptmp_002288"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGV7850880.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13427..14068
                     /gene="kdgA"
                     /locus_tag="ID725_RS11445"
                     /old_locus_tag="pgaptmp_002289"
                     /gene_synonym="eda"
     CDS             13427..14068
                     /gene="kdgA"
                     /locus_tag="ID725_RS11445"
                     /old_locus_tag="pgaptmp_002289"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGV7850881.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14124..15302)
                     /gene="purT"
                     /locus_tag="ID725_RS11450"
                     /old_locus_tag="pgaptmp_002290"
     CDS             complement(14124..15302)
                     /gene="purT"
                     /locus_tag="ID725_RS11450"
                     /old_locus_tag="pgaptmp_002290"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGV7850882.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            15436..15726
                     /gene="yebG"
                     /locus_tag="ID725_RS11455"
                     /old_locus_tag="pgaptmp_002291"
     CDS             15436..15726
                     /gene="yebG"
                     /locus_tag="ID725_RS11455"
                     /old_locus_tag="pgaptmp_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGV7850883.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            15793..16149
                     /gene="yebF"
                     /locus_tag="ID725_RS11460"
                     /old_locus_tag="pgaptmp_002292"
     CDS             15793..16149
                     /gene="yebF"
                     /locus_tag="ID725_RS11460"
                     /old_locus_tag="pgaptmp_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGV7850884.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16476..17135
                     /gene="yebE"
                     /locus_tag="ID725_RS11465"
                     /old_locus_tag="pgaptmp_002293"
     CDS             16476..17135
                     /gene="yebE"
                     /locus_tag="ID725_RS11465"
                     /old_locus_tag="pgaptmp_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HGV7850885.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17344..19404
                     /gene="ptrB"
                     /locus_tag="ID725_RS11470"
                     /old_locus_tag="pgaptmp_002294"
     CDS             17344..19404
                     /gene="ptrB"
                     /locus_tag="ID725_RS11470"
                     /old_locus_tag="pgaptmp_002294"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGV7850886.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(19401..20063)
                     /gene="exoX"
                     /locus_tag="ID725_RS11475"
                     /old_locus_tag="pgaptmp_002295"
     CDS             complement(19401..20063)
                     /gene="exoX"
                     /locus_tag="ID725_RS11475"
                     /old_locus_tag="pgaptmp_002295"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGV7850887.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADMTGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20087..20743)
                     /gene="yobB"
                     /locus_tag="ID725_RS11480"
                     /old_locus_tag="pgaptmp_002296"
     CDS             complement(20087..20743)
                     /gene="yobB"
                     /locus_tag="ID725_RS11480"
                     /old_locus_tag="pgaptmp_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGV7850888.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(20845..21075)
                     /gene="holE"
                     /locus_tag="ID725_RS11485"
                     /old_locus_tag="pgaptmp_002297"
     CDS             complement(20845..21075)
                     /gene="holE"
                     /locus_tag="ID725_RS11485"
                     /old_locus_tag="pgaptmp_002297"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGV7850889.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21214..21588
                     /gene="yobA"
                     /locus_tag="ID725_RS11490"
                     /old_locus_tag="pgaptmp_002298"
     CDS             21214..21588
                     /gene="yobA"
                     /locus_tag="ID725_RS11490"
                     /old_locus_tag="pgaptmp_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGV7850890.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21592..22464
                     /gene="copD"
                     /locus_tag="ID725_RS11495"
                     /old_locus_tag="pgaptmp_002299"
     CDS             21592..22464
                     /gene="copD"
                     /locus_tag="ID725_RS11495"
                     /old_locus_tag="pgaptmp_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGV7850891.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            22477..22818
                     /gene="yebY"
                     /locus_tag="ID725_RS11500"
                     /old_locus_tag="pgaptmp_002300"
     CDS             22477..22818
                     /gene="yebY"
                     /locus_tag="ID725_RS11500"
                     /old_locus_tag="pgaptmp_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGV7850892.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            23214..23870
                     /gene="pphA"
                     /locus_tag="ID725_RS11505"
                     /old_locus_tag="pgaptmp_002301"
     CDS             23214..23870
                     /gene="pphA"
                     /locus_tag="ID725_RS11505"
                     /old_locus_tag="pgaptmp_002301"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGV7850893.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(23871..24062)
                     /gene="yebW"
                     /locus_tag="ID725_RS11510"
                     /old_locus_tag="pgaptmp_002302"
     CDS             complement(23871..24062)
                     /gene="yebW"
                     /locus_tag="ID725_RS11510"
                     /old_locus_tag="pgaptmp_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGV7850894.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(24167..24403)
                     /gene="yebV"
                     /locus_tag="ID725_RS11515"
                     /old_locus_tag="pgaptmp_002303"
     CDS             complement(24167..24403)
                     /gene="yebV"
                     /locus_tag="ID725_RS11515"
                     /old_locus_tag="pgaptmp_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGV7850895.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(24521..25960)
                     /gene="rsmF"
                     /locus_tag="ID725_RS11520"
                     /old_locus_tag="pgaptmp_002304"
     CDS             complement(24521..25960)
                     /gene="rsmF"
                     /locus_tag="ID725_RS11520"
                     /old_locus_tag="pgaptmp_002304"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGV7850896.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(26041..28674)
                     /gene="yebT"
                     /locus_tag="ID725_RS11525"
                     /old_locus_tag="pgaptmp_002305"
     CDS             complement(26041..28674)
                     /gene="yebT"
                     /locus_tag="ID725_RS11525"
                     /old_locus_tag="pgaptmp_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGV7850897.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(28643..29926)
                     /gene="yebS"
                     /locus_tag="ID725_RS11530"
                     /old_locus_tag="pgaptmp_002306"
     CDS             complement(28643..29926)
                     /gene="yebS"
                     /locus_tag="ID725_RS11530"
                     /old_locus_tag="pgaptmp_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGV7850898.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            30056..30553
                     /gene="msrC"
                     /locus_tag="ID725_RS11535"
                     /old_locus_tag="pgaptmp_002307"
     CDS             30056..30553
                     /gene="msrC"
                     /locus_tag="ID725_RS11535"
                     /old_locus_tag="pgaptmp_002307"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGV7850899.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            30650..31348
                     /gene="proQ"
                     /locus_tag="ID725_RS11540"
                     /old_locus_tag="pgaptmp_002308"
     CDS             30650..31348
                     /gene="proQ"
                     /locus_tag="ID725_RS11540"
                     /old_locus_tag="pgaptmp_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGV7850900.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            31368..33416
                     /gene="prc"
                     /locus_tag="ID725_RS11545"
                     /old_locus_tag="pgaptmp_002309"
     CDS             31368..33416
                     /gene="prc"
                     /locus_tag="ID725_RS11545"
                     /old_locus_tag="pgaptmp_002309"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGV7850901.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            33608..34489
                     /gene="htpX"
                     /locus_tag="ID725_RS11550"
                     /old_locus_tag="pgaptmp_002310"
     CDS             33608..34489
                     /gene="htpX"
                     /locus_tag="ID725_RS11550"
                     /old_locus_tag="pgaptmp_002310"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGV7850902.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(34535..35908)
                     /gene="yebQ"
                     /locus_tag="ID725_RS11555"
                     /old_locus_tag="pgaptmp_002311"
     CDS             complement(34535..35908)
                     /gene="yebQ"
                     /locus_tag="ID725_RS11555"
                     /old_locus_tag="pgaptmp_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7850903.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            36085..36876
                     /gene="kdgR"
                     /locus_tag="ID725_RS11560"
                     /old_locus_tag="pgaptmp_002312"
     CDS             36085..36876
                     /gene="kdgR"
                     /locus_tag="ID725_RS11560"
                     /old_locus_tag="pgaptmp_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGV7850904.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(37020..37259)
                     /gene="yobH"
                     /locus_tag="ID725_RS11565"
                     /old_locus_tag="pgaptmp_002313"
     CDS             complement(37020..37259)
                     /gene="yobH"
                     /locus_tag="ID725_RS11565"
                     /old_locus_tag="pgaptmp_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGV7850905.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            37419..37562
                     /gene="mgrB"
                     /locus_tag="ID725_RS11570"
                     /old_locus_tag="pgaptmp_002314"
     CDS             37419..37562
                     /gene="mgrB"
                     /locus_tag="ID725_RS11570"
                     /old_locus_tag="pgaptmp_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGV7850906.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            37637..37924
                     /gene="yebO"
                     /locus_tag="ID725_RS11575"
                     /old_locus_tag="pgaptmp_002315"
     CDS             37637..37924
                     /gene="yebO"
                     /locus_tag="ID725_RS11575"
                     /old_locus_tag="pgaptmp_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGV7850907.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         9741 a         9372 c         9814 g         9116 t
ORIGIN      
        1 cagttcatcg agacacctcg caagtttttt tcatccttcg ctggatatct atccagcatt
       61 tttttatcat acagcattat ctttgattca ttacgcagga gcgtcatgtg ataggcagac
      121 tcagaggcat catcattgaa aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag
      181 gctatgaagt gcatatgccg atgacctgtt tttatgaact ccctgaagcg ggtcaggaag
      241 cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca actgctgtac ggttttaaca
      301 ataaacaaga gcgcacgttg ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt
      361 tggcgctggc gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg
      421 aagaagtggg ggcactggtg aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga
      481 ttgttgaaat gaaagaccga tttaaaggtt tgcatggcga tctctttacg ccagccgccg
      541 acctggtact cacgtctcct gccagcccgg cgaccgacga tgctgaacaa gaagcggttg
      601 ccgcgctggt ggcgctgggc tataaaccac aagaagcaag ccgcatggtg agcaaaatcg
      661 ctcgccctga cgccagcagt gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag
      721 gtaaaggatg attgaagcag accgtctgat ttctgccggt accactttgc cggaagatgt
      781 agcagatcgc gccattcgcc ctaaattact ggaagagtat gttggtcagc cgcaggttcg
      841 ttcacagatg gagattttca tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt
      901 gttgattttt ggtcctccgg ggttgggtaa aactacgctt gccaacattg tcgccaatga
      961 aatgggcgtt aatttacgca cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc
     1021 tgcgatgctc actaaccttg aaccgcatga cgtgctgttt attgatgaga tccaccgttt
     1081 atcgccagtt gttgaagaag tgctgtaccc ggcaatggaa gactaccaac tggatatcat
     1141 gattggtgaa ggtccggcgg cacgctccat taaaattgat ttgccgccgt ttaccctgat
     1201 tggtgcaacc acgcgcgcag gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt
     1261 gcaacgtctg gagttttatc aggtgccgga tctgcaatat atcgtcagtc gcagcgcacg
     1321 ctttatgggg cttgagatga gtgatgacgg cgcgctggaa gttgctcgtc gcgctcgcgg
     1381 tacgccgcgc attgccaacc gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca
     1441 cgatggcacc atctcggcag atatcgctgc tcaggcgctg gatatgttga atgtcgatgc
     1501 tgaaggtttc gattatatgg accgcaaatt gttgctggcg gtaatcgata agttctttgg
     1561 tgggccggta ggtctggata accttgcggc agccattggc gaagaacgtg aaaccattga
     1621 ggatgtgctg gaaccttatt tgattcagca aggctttttg cagcgtacac cgcgtgggcg
     1681 tatggcgacg acgcgggcgt ggaatcactt tggcataacg ccgccagaaa tgccgtaagt
     1741 cggattggtc gctcgcaccg catccgacat tggcagtgtg cctgatgcga cgctattcgc
     1801 gtcttatcag gcatatacca gagatagcga accgtaggtc ggaaaaggcg ctcgcgccgc
     1861 atccgacatt ggcagtgtgc ctgatgcgac gctgttcgcg tcttaccagg cctaccagag
     1921 atagcgaacc gtaggtcgga taaggcgctc gcgccgcatc cgacaaatgt gttcagcgat
     1981 agattagctg gcctgctttt tcatcatact gataataaat aacagtgccg cacatagcac
     2041 caccgacgga cccgccggcg tatcgtaaaa cgcggaaaag gttaaaccgt cagttactgc
     2101 caccatcccc accaaaacag cgacaccagc catctgttcc ggcgtacggg caaagcgacg
     2161 cgcagtagct gcaggaataa tcagcaacga agtaataatc aacgcaccga caaatttcat
     2221 cgctacacca atcgtcaatg ccgtcaccag catcaacaac aatttcactc gctgtaattt
     2281 cacaccatca acaaacgcca gatccgggct aatcgtcatc gacagcaaat tgcgccattg
     2341 ccagaacaaa atagccacca cgatgaccac gccaattgca atagagatga gatcttctgg
     2401 cgtcactgcc agtaaatcac cgaacaggta agccatcaaa tcaacacgaa tgttagacat
     2461 cagactaacg accaccaggc ccagcgacag ggcactgtgc gccataatcc ctaataacgt
     2521 gtcgatcgcc agctgtggac gcttctccag ccataccaga ccgcccgcca gcagcagcgt
     2581 aacggcaatc accgcataga atggattcac gtccagcaac aaaccaaacg cgacaccaag
     2641 taatgaggca tgagccagcg tatcaccgaa ataagacata cgacgccaga ctacaaacga
     2701 acccagcgga cccgcggcac aggcgagcat gatcccggct aaccaaccgg gaaataataa
     2761 ttcaatcatg agcgatcatt tccccgacgc aaaacaattc gtccctgtaa atcgtgacga
     2821 tgattatgat gatggcgata gatacccagt tgttcagcac cacgaggacc aaacattgaa
     2881 ataaactccg gatgcaggga aacaacttcc ggtgtgccgg aacaacaaat gtgatgattc
     2941 aggcacagca cttcatcggt ttttgccatc accagatgaa gatcgtgaga aaccattaaa
     3001 acgccacaat ccagttcacg acgcagttgg tcaataaggt catataacgc cacctggcca
     3061 tttacatcca cgccttgagt gggttcatcc agcactaata attgcgggcg atttaacaat
     3121 gctcgcgcta acagtacacg ctgcgtttcg ccaccagaga gcttttgcat cggtgcgtta
     3181 atcaggtgcc cggcctggac acgtttcagt gcaggcaaaa tatcttcttt atgtgtacca
     3241 gggcgtaagc gtaaaaaacg gtttacggtc agtggcaacg tggtgtcgag atacagcttc
     3301 tgtggtacat agccgatgcg cagttttccg ttgcgcttga taaccccttc atcgggtgtt
     3361 accagcccga gcactacccg taccagtgtc gacttacctg cgccatttgg cccaagtaaa
     3421 gtcaaaattt ttccaggttt aagttccagc gacacatcag agaggacgcg gcgttggcca
     3481 aaagaaaccg agacattttc cagggaaacc agacttgtca tgttaatttt agtcttgcag
     3541 tagtcatgaa atgttataat atcacacttc tcatattcat tacgatgatt ggtcgcatta
     3601 tgttacataa aaaaacgctt cttttcgcag cattattcgc cgctctctgg ggcggtgcaa
     3661 cacaggccgc agatgccgcc gttgtcgctt cgcttaaacc cgttgggttc atcgcttctg
     3721 ccattgctga tggggtaaca gaaacggagg ttttacttcc tgacggcgct tcagaacatg
     3781 attattcact gcgtccatcg gatgtaaaac gcttacagaa cgcggactta gtcgtttggg
     3841 ttggcccgga gatggaagcg tttatgcaaa aaccggtaag caaattacca gaagcgaagc
     3901 aggtaacgat tgcgcagctt gaagatgtga aaccgctact gatgaaaagt attaacggcg
     3961 atgatgatga tcacgaccac gcggaaaaaa gtgacgaaga tcaccatcac ggcgatttca
     4021 acatgcatct ttggctttcc ccagagatag cgcgggctac agcggttgca atccatggaa
     4081 aattagtgga acttatgccg caaagtcgag ccaaacttga cgccaacctg aaggattttg
     4141 aggcacaatt agcctcaacc gaaacgcagg ttggtaacga gctcgcgccg ctcaagggga
     4201 aaggttattt cgtttttcac gatgcttacg gctatttcga aaaacagttc ggactgacac
     4261 cgcttggtca ttttaccgtt aaccctgaga ttcaacctgg cgcgcagcgt ttacatgaaa
     4321 taagaacaca gttggttgag caaaaagcaa cctgcgtttt tgctgagcca cagttcaggc
     4381 cagcggtcgt tgagagcgtc gcacgaggga catccgttcg tatgggaacg ttggatccct
     4441 tggggacgaa tatcaaactg ggtaaaacaa gctattcaga attcctgagt caattagcca
     4501 accagtatgc gagctgcctg aaaggagatt aatgaggaag tgattacgtg caacagatag
     4561 cccgctctgt cgccctggcg tttaataatt taccgcgacc acaccgcgtt atgttggggt
     4621 cgctcaccgt tcttactctg gccgtcgctg tctggcggcc ctatgtttat caccgcgatg
     4681 ccacgccaat tgtcaaaacc attgagctgg aacagaacga aattcgttcg ctcttacctg
     4741 aagccagtga gccgattgat caagctgcac aagaagatga agccattccc caggacgaac
     4801 tggatgacaa aatcgccggt gaagcgggcg tgcatgaata tgttgtttcc actggcgata
     4861 cgctaagcag cattctcaat cagtatggta ttgatatggg tgatatcacc caactggctg
     4921 cggccgacaa agaattgcgt aacctgaaaa tcggtcaaca actctcctgg acattaaccg
     4981 cggacggcga actgcagcgc ctcacctggg aagtgtctcg tcgtgaaacc cgaacctatg
     5041 accgtactgc cgctaacggt tttaaaatga ccagcgaaat gcagcaagga gagtgggtta
     5101 acaatctgct gaaaggtacc gtcgggggga gctttgttgc cagcgccaga aacgccggtt
     5161 tgaccagcgc cgaagtgagc gcagtgatta aagccatgca gtggcaaatg gatttccgca
     5221 aactgaaaaa aggcgatgaa tttgcggtat taatgtctcg agaaatgctt gatggtaaac
     5281 gtgagcaaag ccagctgctg ggcgtacgtt tgcgttcaga aggtaaagat tattacgcta
     5341 tccgcgctga agatggcaaa ttctacgatc gtaacggtac tggtctggcg aaaggattct
     5401 tgcgattccc gacggcgaaa cagttccgta tctcgtctaa ctttaacccg cgtcgtacta
     5461 atccggtgac cggtcgcgtt gcaccacaca gaggtgttga tttcgccatg ccgcagggta
     5521 cgccagtgct ttcagtgggt gacggtgaag tggtggttgc caaacgcagt ggcgcagcag
     5581 gttattatgt ggctattcgt catggtcgca gctacaccac gcgttatatg cacttgcgca
     5641 agattctggt gaaaccgggg cagaaggtga aacgtggcga ccgtatcgcg ctttccggta
     5701 ataccggacg ttcaaccggg ccgcatctgc actatgaagt atggataaac cagcaggccg
     5761 taaacccgct gacggcaaaa ctgccgcgta ccgaagggct gaccggctcc gatcgtcgcg
     5821 aattcctggc gcaggccaaa gagattgtgc cgcagctacg gtttgattaa ttaacatcca
     5881 ttcgcagccg gtacgcagtc agtaccggct ttttttattt ggtgcggggc aagttgcgcc
     5941 gctacactat caccagattg atttttgcct tatccgaaac tggaaaagca tggaaacgaa
     6001 aaaaaataat agcgaataca ttcctgagtt tgataaatcc tttcgccacc cgcgctactg
     6061 gggagcatgg ctgggcgtag cagcgatggc gggtatcgct ttaacgccgc caaagttccg
     6121 tgatcccatt ctggcacggc tgggacgtat tgccggacga ctgggaaaaa gctcacgccg
     6181 tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt agtgaagctg aacgcgaagc
     6241 gattgttgat gagatgtttg ccaccgcgcc gcaagcgatg gcaatgatgg ctgagttggc
     6301 aatacgcggg ccggagaaaa ttcagccgcg cgttgactgg caagggctgg agatcatcga
     6361 agagatgcgg cgtaataacg agaaagttat ctttctagtg ccgcacggtt gggccgtcga
     6421 tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg gcagcgatgt tccataatca
     6481 gggcaacccg gtttttgatt atgtctggaa cacggtgcgt cgtcgctttg gcggtcgtct
     6541 gcatgcgaga aatgacggta ttaaaccatt catccagtcg gtacgtcagg ggtactgggg
     6601 atattattta cccgatcagg atcatggccc agagcacagc gaatttgtgg atttctttgc
     6661 cacctataaa gcgacgttgc ccgcgattgg tcgtttgatg aaagtgtgcc gtgcgcgcgt
     6721 tgtaccgctg tttccgattt atgatggcaa gacgcatcgt ctgacgattc aggtgcgccc
     6781 accgatggat gatctgttag aggcggatga tcatacgatt gcgcggcgga tgaatgaaga
     6841 agtcgagatt tttgttggtc cgcgaccaga acaatacacc tggatactaa aattgctgaa
     6901 aactcgcaaa ccgggcgaaa tccagccgta taagcgcaaa gatctttatc ccatcaaata
     6961 aaaaagcctc tcgcgaggag aggccttcgc ctgatgataa gttcaagttt gcttcagaat
     7021 attcgaaatc tgttgaacta tcattgaact gtaggccgga tgtggcgttt tcgccgcatc
     7081 cggcaacgta cttactctac cgttaaaata cgcgtggtat tagtagaacc cacggtactc
     7141 atcacgtcgc cctgggtgac aatcaccagg tcaccagaca tcaagtaacc tttatcgcgc
     7201 agcagattaa ccgcttcgct ggcagctgct acgccgtcat tagcgctatc aaagtgcacc
     7261 ggcgtaacgc cacgatagag agcagtcagg ttcagcgtac gttcatggcg cgacatggcg
     7321 aaaattggta gaccagagct gatacgggag gtcatcagcg cggtacgacc cgattcggtc
     7381 atggtaatga tcgccgtaac gcctttcagg tggttagctg cgtacattgc tgacatggca
     7441 atagcttctt ccacattgtc gaactgaacg tccagacggt gtttagaaac gttgatgctc
     7501 gggatttttt ccgcacccag gcaaacgcgc gccatggctg caacagtttc tgacggatac
     7561 tgcccagcgg cagtttctgc agacagcatc acagcgtcag taccatccag aacggcgttt
     7621 gctacgtcca tgacttctgc acgcgtcggc atcgggttag taatcattga ctccatcatc
     7681 tgggtcgccg tgattaccgc tcggtttagc tgacgcgcac gacggatcaa cgctttctga
     7741 atgccgacca gttccgggtc gccaatttcc acaccgaggt cgccacgtgc aaccattacc
     7801 acgtcagagg cgaggatgat gtcatccatt gcatcctggc tgcaaacggc ttccgcacgt
     7861 tcaaccttgg caacaatttt cgcatcacat cctgcatcgc gtgccaggcg acgggcatag
     7921 ttcagatctt cgccacagcg tgggaaggag acagccaggt aatctacgcc aatcaacgcc
     7981 gcagtcttaa tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa accgccgcca
     8041 agtttgttga tacctttatt gttggagagg ggaccaccga cggtgacttc ggtgaacact
     8101 ttcatgccct gaacttccag tacttttaac tggacgcgac catcgtccag cagcaggatg
     8161 tcaccaggca cgacgtcagc aggcaggcct ttgtagttga taccgacttt ttctttgtcg
     8221 ccttcacctt tacccaggtt ggcgtcgagc aggaatttat ccccaatatt gaggaaaact
     8281 ttgccttctt taaaggtgga tacacggatt ttgggcccct ggaggtcacc cagaatagcc
     8341 acatgacgcc ccagttttgc ggcaatctca cgaactttat ccgcgcgcat tttgtgatct
     8401 tcaggcgagc cgtgagaaaa gttcatacgt acaacgttgg cacccgccgc gataactttt
     8461 tcaagattat tatcgcgatc tgttgctggg cctaacgtgg taacgatttt tgttctgcga
     8521 agccttctgg acatgtaata ctccgttgac tgaaacaacc aggtgttgct tgaacatgaa
     8581 atccgaatga aataacgccg cgatggcgat aaaataatct cattcggatg tcgtataaga
     8641 ttagaacagt gacagtcgct tttagcgatc gtcacttaaa ttaagtaact gcttatcaaa
     8701 acgcgattcc ttcagcgctt ccttgacccg cttcaagtta tctctgaatt ttgcaccgcg
     8761 tcgcaaagta aatcctgtcg ccagcacatc tatcacggtc agctgtgcaa gtcgagaaac
     8821 catgggcata taaatgtcag tatcttccgg tacgtcgagg gtaattgcca gcgttgcttc
     8881 ccgggcgagc ggggtacccg cagaggtgag ggcaatcacc atagcgtcgt tttcgcgtgc
     8941 cagctgcgcc agctcgacca gattttttgt tcttccggtg tgagaaatca gcaccaccac
     9001 gtctccgtcg ctacaattca tacaactcat gcgttgcagc acaatatcat cggagtacac
     9061 caccggaaca ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg cggctgaaga
     9121 gcctaatccg aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg cgcggttgat
     9181 ggcagattta tccagtgaat gacggacatg atcgagcgtt gccattgcgg actcaaatat
     9241 tttccctgtg tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa catagggagt
     9301 gccattcgcc agactctgtg ccagatgaag tttaaaatca ggaaaaccgc gcgtgtccat
     9361 gctgcgacag aaacgattca ccgtcggttc gctaacattg gcttccagtg ccagagcagc
     9421 aatactcgaa tggatcgcgt tatcgggcga agccagaatg acctcggcaa ctttgcgctc
     9481 tgatttgctc aaatgttcca gctgagactg gattttttcc agcatattca tgatgtaaag
     9541 agactcacgg gtaatgacga tttccgcact gaaagaaatc gaaatgcagt tttgtcagat
     9601 attacgcctg tgtgctgtgt taatgacaaa agcagataaa aaagttgtta ttttttttca
     9661 taacatgatc agtgtcagat ttttacccaa tggaaaacga tgattttttt atcagttttg
     9721 ccgcactttg cgcgctttta ccgtaatcgc acgggtggat aagcgtttac agttttcgca
     9781 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
     9841 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggtctgtga
     9901 cttggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
     9961 tcaactggaa aaagccggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    10021 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    10081 agaaaccatt gatgaaggtt tatgggacac cctgagcgca cgtctggatt tttgtaatct
    10141 cgatgtcaat gacactgctg cattcagccg tctcggcgcg atgctggatc aaaaaaatcg
    10201 tatcaccatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    10261 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    10321 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    10381 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    10441 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    10501 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    10561 gatgcgcgac atgatccaga accacctgct gcaaattctt tgcatgattg cgatgtctcc
    10621 gccgtctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtctct
    10681 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    10741 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaagagcag
    10801 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    10861 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    10921 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    10981 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    11041 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    11101 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    11161 tattcaggca ctgtttgtac gtcgcgatga agtggaagaa gcctggaaat gggtagactc
    11221 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    11281 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    11341 gtaatatctg cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    11401 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    11461 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    11521 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    11581 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    11641 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    11701 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaagacaaag cctctttgaa
    11761 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgctca
    11821 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    11881 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    11941 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggctg tcgggctgtc
    12001 ccataacatg tttgatggtg ctctgttcct cggggtgtgc gacaagattg tcccgggtct
    12061 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    12121 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    12181 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    12241 tactttctac ggtactgcca acaccaacca gatggtggtg gagtttatgg ggatgcagtt
    12301 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    12361 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    12421 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    12481 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    12541 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    12601 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    12661 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    12721 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    12781 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    12841 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    12901 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    12961 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    13021 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    13081 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    13141 agctatccac gtaacaccag aagcttacga tggcgggctg ctggcaaaag tgcgcgacgg
    13201 ggacatcatt cgtgtgaatg gacagacagg cgaactgacg ctgctggtag acgaagcgga
    13261 attggctgct cgcgaaccac acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    13321 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    13381 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    13441 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    13501 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    13561 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    13621 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    13681 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    13741 cgaagggact attccgctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    13801 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    13861 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    13921 tccggctaac taccgtgact acctggcgct gaaaagcgtg ctgtgcatcg gtggttcctg
    13981 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    14041 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatcgagg atcgggcatt
    14101 tttgtagcgt ttatttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    14161 gcgcgttcaa tggcgtcaac aacactctct gcagtagcca gtgccacccc cagacgacgg
    14221 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    14281 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    14341 gcaggaccat actgacggat cccgccaacc ggaagtccga ggaaggcacg tacatgcagg
    14401 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    14461 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    14521 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    14581 gggctcattt gctgtggttg ccaggattca cagtagtcgc catcttcctg gcgatgacct
    14641 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    14701 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    14761 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    14821 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    14881 aaaaggcttt cgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    14941 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    15001 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    15061 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    15121 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    15181 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    15241 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    15301 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    15361 tttattgctg gtgttgatct ctcaaccgaa aagaaatata ctgtatagaa tcacagttat
    15421 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    15481 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    15541 gatctgcttg acacctggct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    15601 gccctttcgc tatggctggc agaacaaaaa gatgtgctga gcaccattct gaaaaccggc
    15661 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    15721 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    15781 atggagaaaa acatgaaaaa aagaggggcg tttttagggc tgttgttggt ttctgcctgc
    15841 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    15901 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    15961 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc atgggtcagt
    16021 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt acgtggtaaa
    16081 agtgccgata ttcattacca ggtcagcgtg gactgcaaag cgggaatggc ggaatatcag
    16141 cggcgttaat gggttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    16201 tccgccccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    16261 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    16321 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    16381 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atcctctatg
    16441 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    16501 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    16561 aacttttagt gccgggcgca ttaggcggac tggctggact gctggtcgca aataaatcag
    16621 cacgtaaact tcttacgaaa tatggcacca acgcgttgct gattggcggc ggagcggtag
    16681 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    16741 cgcagtttgg ctcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    16801 tagtctttgc cgctaaaagt gatggtcata ttgatgccaa agaacgtgcg gcaatcgacc
    16861 agcaattgcg tgaagccggc gtggaagagc aggggcgggt actcattgag caggcaatcg
    16921 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    16981 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    17041 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    17101 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    17161 tgactatttc gggctatcca gaacagcaca gccatttgaa caggctgatt ttctctgtct
    17221 gcataacatt cgatattcgc gccattacct gtcatcatct aagcaatgac tgccctgttt
    17281 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttcaaccag aaagaacaat
    17341 aacatgctac caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc
    17401 atcgataatt actactggct gcgggacgat acgcgttctc agccggaagt cctggactac
    17461 ctgcaacaag aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat
    17521 cgcatcttaa aggaaatcat cgaccgcatt ccgcaacgag aagtttctgc gccctacatc
    17581 aaaaatggct accgctaccg gcatatttat gaaccaggct gtgaatatgc tatctaccag
    17641 cgtcaatcgg cgttcagtga agagtgggac gagtgggaaa tattgctcga tgccaacaag
    17701 cgcgcggctc atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc
    17761 attatggcgc tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat
    17821 ctggaaacag gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg
    17881 gcaaatgact cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat
    17941 caggtctggc gtcacgccat cggtacgcca gcatcgcaag ataaactgat ttacgaagaa
    18001 aaagacgata cctattacgt cagcctgcat aaaacgacct caaagcacta tgtagtcatt
    18061 catttggcca gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggcagatgcc
    18121 gagccgtttg tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag
    18181 catcgttttt atctgcgttc caaccgccac ggcaaaaact ttggcttata ccgtacccgt
    18241 atgcgtgatg agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa
    18301 gggtttacgc tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt
    18361 ttgcgccaaa ttaaccgcaa gacccgggaa gtcattggta ttgcctttga tgatccagcc
    18421 tatgtgacct ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat
    18481 tcttccatga ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt
    18541 gtattaaaac aaacagaagt tcctggtttt gatgcggcga attaccgcag tgaacacctg
    18601 tggatagtcg cccgtgatgg tgtcgaagtt ccggtttcgt tggtctacca tcgcaatcat
    18661 tttcgcaaag gacacaaccc gctgctggtg tatggctacg gttcttacgg cgcaagtatt
    18721 gatgccgatt tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt
    18781 gtccatgttc gcggcggcgg tgagctgggg caacaatggt acgaagacgg taaatttctg
    18841 aagaagaaaa atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc
    18901 tatggctctc cttcgctttg ttatgcgatg ggcgggagtg cggggggcat gttgatgggc
    18961 gttgcaatta atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt
    19021 gatgttgtaa caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag
    19081 tgggggaatc cgcaggatct gcaatattac gagtatatga aaagctacag cccgtatgac
    19141 aacgtcaccg cacaggctta tccacattta ctggtaacga ccggtttaca cgattctcag
    19201 gtgcaatatt gggaaccggc aaaatgggtc gctaagttgc gcgagctgaa aaccgataac
    19261 catcttttat tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggtcgcttt
    19321 aaatcgtacg aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca
    19381 ttacccgcta ggcctgcgga ttaggtattt tccagataat gtttcagtgt taaacgcagc
    19441 tccgggctca tgctgtccag gttattaaac aaccagcgca gatagcccgg atcgcgttcg
    19501 gcaacgtcgg aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt catcaacgac
    19561 ggacgtccgg tcatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg
    19621 ataagcaacg cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc
    19681 ggcgtctgta cattgagctt ccgtgtttta tataacgcca tattgctgta cttgatccct
    19741 ggccacaaac gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt
    19801 acgcggcggt caaagctggc gttatgcgcg acataccatt cactaccgta atagtgtggg
    19861 atcacatctt caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc
    19921 atcgcttgtg gactaatagg acgatcgggg tgcaccaggt ggctcatggg gttgacgatt
    19981 ttcccgtcaa tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt
    20041 tctgtatcga taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa
    20101 tgatatcgcc ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat
    20161 cggcgcgaac gatgagacga ccatgttcat cccatagtgc actgttacca cgggcatttg
    20221 ccatcagcac agcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgatgcca
    20281 gcaggtcggg ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat
    20341 ccataccttg cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg
    20401 caccatgact ctgatggtag atcccaggcg tttttcgcca cggtgcgaac accgcaattc
    20461 cacgaataaa gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgccagg
    20521 tcgttgcggc ataacaaagc gggtctaaca gtgagagatc gggtggggca ggaagggcac
    20581 gtcgcgaata atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt
    20641 gcctggcggc ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt
    20701 tacgaggctc atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa
    20761 aatgactgca aattatgggt gcggtttaga aaatactcat aatttagcag gcgttatgta
    20821 agaaagtgta actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag
    20881 attgaccgaa gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc
    20941 aggctgttca cgttcaaccg cttcagcgat cactggcata ttgtagcgtt ctttaaatgc
    21001 caccccggcc gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc
    21061 cagattcttc agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag
    21121 cctggatggc aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct
    21181 gtaaaaaagt tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc
    21241 tacgcgctgg caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat
    21301 ttaacgcatc agtatcctgc ggcaaacgcg caagtgacag ctgcaccgca ggcaatcact
    21361 ttaaacttct cggaaggtgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa
    21421 aacgaaaata ttaaaacatt accggcgaag cgaaatgagc aggaccaaaa gcgactgatt
    21481 gttccactgg ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg
    21541 gtggacgggc ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg
    21601 tttacctgga tcgcgctgcg atttatccat ttcacctcgc tgatgctggt ctttggcttc
    21661 gcaatgtacg gtgcctggct ggcaccctta acgattcgtc gcttgctgac gaagcgtttt
    21721 ctgcgtttac aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc
    21781 gttcagggcg ggttgatggg aacgggatgg aaagatgtat tttcgccaaa catttggcag
    21841 gctgttttgc aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta
    21901 acgttgatcg tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact
    21961 accgcgcaat ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaacg
    22021 gcgaaaatcc atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg
    22081 ggattactgc cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acaccaggct
    22141 attcaggcgc tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca
    22201 agcggcgtgc ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg
    22261 ggccaactct tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct
    22321 aatcgttatg ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt
    22381 gtgtggatga ctaaactcga atgggcgata ggtgcggtag tgctgctgat catcagcctg
    22441 cttgcgactc tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg
    22501 tttctgttac tcaccagttc tgcagcggcg ctggctgcac cgcaggtgat taccgtcagc
    22561 cgttttgaag tgggtaaaga caaatgggcg tttaatcgcg aagaggtgat gctgacttgc
    22621 cgaccgggta atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac
    22681 gatgtcgcac agaaggaagt tgccagtggg aagactaaag cccaacccat ttcggtgatt
    22741 cagattgatg atcccaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga
    22801 gctgaaaaac tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct
    22861 cagttctttc accagaacgg gcggttttta acatttaagc tgatgaccac cgcgcttttt
    22921 attgaccatt ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa
    22981 ggcaactaag cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagccattt
    23041 ccctggaccg aatacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca
    23101 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
    23161 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
    23221 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
    23281 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
    23341 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
    23401 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
    23461 tggcgatgga tgcgctggca tcccagcaga tgtctttgtg gttgatgaat ggcggcgact
    23521 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
    23581 agcatttgcc ctttattctt gaagtacaca gccgcaccgg caaacatgtt attgctcatg
    23641 ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat caggtcttgt
    23701 ggagccgctc gcgattaagt gaacgccaaa aagggcaggg aattacaggt gctgatcatt
    23761 tctggtttgg tcatacaccg ttgcgccatc gcgtggatat tggcaacctg cattatattg
    23821 ataccggcgc tgtctttggg ggcgaactga ctttcgtgca attgcaataa ttaaaaatca
    23881 ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg aaaacaaccg
    23941 ccatgacgga tccgttgatc gctcatagaa taaagacact gctgttccgt gttgtagaca
    24001 tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac cagcgcgaac
    24061 atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt acaacggggg
    24121 aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag gcaagacgca
    24181 taatccaggc atcagattgt tgatcgtaac gggtacggta aaggacagag tgttcgccat
    24241 ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata cataaatcgg
    24301 ggtctgattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg tgtaattcgc
    24361 cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg ctctccattt
    24421 agctgccggt tgtggtacga ccagtccgat cgcaccattt ttcagcctag ttgaggccgc
    24481 cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa
    24541 gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt
    24601 tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc actggcgcgg
    24661 cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcttgcggt gtcagttcaa
    24721 aagcgttctc gttgtcgggg gaggcaaggg caataaccgc ttcatgctgc cagcgataac
    24781 ctttgttgtg cgtttcggca agtttaatcc ccaggcgaga aaatcggact ttaccgatca
    24841 gggcttcaat gcctaccgga aacaaccaca cttctttgtc gcgctgccag agtcgcaggt
    24901 ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtgcg gcttcgcgat
    24961 ctttcaccgg gctgaacggg aaattgccca ctttgtattt tggggtgggt aaggcgggaa
    25021 tggcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa atttgtggga
    25081 aaacatgcaa aaagccttct tcggtcagcg ctttgtttgc accagggaag agatcgccaa
    25141 gtggtaaaaa ctctactgcg tcggggtaag tctctttaag ccacaggcaa acggattcgt
    25201 tttcttcccg gtttaaggta caggtcgagt aaaccagtgt accgccagag cgtaatgcat
    25261 gaaaggcact gtcgatcagt tcccgttggg tagctgcgat ttcctgattg ctttctggtg
    25321 accaattttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag cagggagcgt
    25381 ccagcaaaat ggcatcgaac atttctggca atgccgcacc aaacacgcgg ccatcaaaat
    25441 gtgtgagcgc aacattactg ataccacagc ggctgatatt ggcatgtaac acttttaccc
    25501 gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc gcggcaattt
    25561 gcgtcgtttt ggagcctggc gcagcagcga catccatcac ccgctgtggt gcattaccgt
    25621 cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa aacaggccac
    25681 ttaaatgctc ggcggtacta cccaatggca atgcatcttc atcgtcgcgt tcaatccaga
    25741 aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg gttaatcgca
    25801 ggaaatcagc aacggaggtt ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct
    25861 gacaggcggc aagaaaatca tcaaaagaga gcgtcgaagg cattgcttcg cgcatttgcg
    25921 tcagaaaggc gtccgggaaa taaacggtgt gttgggccac gagcatatac cactggcaaa
    25981 aaaaacaggc gcgcagttta gcataaacgc tccggcgcag gcacgccgga gcagtgggca
    26041 ttatttggga agcgcagttc cccattcacg ccactctttc ggttcacttt cctgcaacag
    26101 gaagtgcttg ccttcctggg ctttcggtgc cagtggcgta cccggaggcg tggcgaaggc
    26161 gatgccgcca cggataaact ggttaaaggt gccggttttc actacgccgc ccgtcagacc
    26221 aaagtccaga ctgtaacccg atgccaacca gaagacggaa ttgttacgca ccaggtgttg
    26281 atagcgttta ctgatgcgca tcgcaatcat cacgcgatct gacaatgtcc ccagcgtcat
    26341 tccggtaacc gtaccgactt ccagaccacg gaacagcaca ggcgtaccga tgcctaacga
    26401 acaggcttcc ggcgcttcaa caataatgct taaaccatcc aggtaacgcg aatcagtaat
    26461 ggtggcctct tgtaattcaa agtcgcgacg aggattgcca cggcctggtt cgacgttgat
    26521 atacggctgg aggatagtat caagatgctc aacgccagct gccgaaattt gcggtgtgac
    26581 cactgagaag cgcgtaccac cgcgagcaaa ggtctggaca tattccggat agagcaccgc
    26641 ctttgcctgt acttcattgc gcgcggtaat cagatccagc gtctggattt gcccgatatc
    26701 aataccgaga tagcgaattg gcatcccgac cgccagtttt ccggcatcga aagcatgaag
    26761 cgtaatctgc ccgccaaccg cacgggccgc tgtttcggaa gcatacagaa tacgcttatc
    26821 gcctttacgc tgactggcgc tggcaccgct gaggttatcg aagctaatgg ctccctttaa
    26881 tgctctggag agtggggatg cctgtacggt caggccacta ccattcagct gaactttcgc
    26941 cccgccttct gcccagaaca cgctattgtt ggtcagaagg ttgcgatact ccggcttaat
    27001 atgcagatcg atatcaaacg cgttagctcg cggacgcacg gtaataactt caccaacttc
    27061 aaatttacgg tacagcacta ccgatcctgc ctgcacatcc ggcagcgtct ctgcactcaa
    27121 actcacggtt gtggtgggta aatcgctaag gctgttctcc agcgcttttt ccagattggc
    27181 atacagtgga tagctggctt tcatctcacc tttatcgccc ggcagaatac gtatcccgcc
    27241 attgatccat tctgaggcgc tggcaccgag aaactcaacg ccatccagcc ccaccttcac
    27301 atcgacacgg ctgttgacga caaatttgct atcgcctttt accagttcgc gatgctgagg
    27361 ctcaatggcg acggtaaagg taacgccttt gctggtgagt ttacgatcaa taacctggcc
    27421 tacctgcacg ccgtgaagaa tgagcggctg accagcatca ataccgtaac tttccggtgc
    27481 tgtcagggtc agtgtcagaa catcaggttc atgcagcagt gctttttcgc ctggcacaac
    27541 aacgaactct ttgcgtggct cgccatcgcc aggcaccaac tcaaaggttt ttccggtcag
    27601 cagggcgctg agattggcat cgctaaggga taatttcggg ttgcgtaatt cgatgcgggt
    27661 attttcccgt aacagcgtaa cgacgctggg atcaacggtc atttctccgg tgactttacc
    27721 accaggattt aaatccagtt tagtcagctg tccgacttcc agcccctgat acattaacgg
    27781 cgtcgagtcg gcggttaatc cggccccact cggcagttcc agttttatta ttacgccacg
    27841 ctggctgtgg gccagatctt catacagacc aaaggtatct tccgcctcgg caggtttcga
    27901 ctcttctggt gaatcgaagg caatcgcacc gttaaccagt gccgccagac tttccagttt
    27961 caccttcgcg ccactgatac tgacgttggc atcaacgcct gaaacgttcc agaaacggct
    28021 accttttttc accaggtcgg taaaacgccg ctcgatcagg acatcaatca ccacgccttg
    28081 tttattggga ttgatggcat agtcgtagac ttttcccacc gggatcttgc ggaaatagac
    28141 caatgaaccg ctgttcagcg aaccgagatc gggagcttgc aggtggatca tcagatcgcc
    28201 gttatccagc cgatacttcg gttgggtatc gagcgcgaca aagtgatcct gctctttacc
    28261 tttacctggc atcatgccga tatagttacc accgacgagg gcgtccagcc cggagacacc
    28321 tgccaacgat gcttttggcg tcaccagcca gaactgagtc tcttcgcgca gcgcatcttt
    28381 catatcggac ttgatgctga ccttgacttc aatcttacga agatcgtcgc tgaggctgat
    28441 atcctgcact gttccgactt caacgccctg ataacgaaca ggcgtacggc ccgggacaat
    28501 accatccgcc gacataaagt cgatggtgac ggtattaccc cggtcctgat aactgtccca
    28561 aatcagccaa ccggcaatca ttagcgcgat gaacggcagc agccagaaag gtgagatacg
    28621 gcgtttattt ttaatctgcg cttcagtcgt cgaagcgggc gtttcctgac tcatgtgcat
    28681 cccaaagtaa gcggctgtcc agccattcca cagcaagaat agtcaaaatt accgctgcgc
    28741 cgaaataaaa cgcagccggt cccatagtaa aagcgaggat ctgatcgcga ttaatcagcg
    28801 acatggttaa agatatgaca aacaggtcga gcatcgacca gcgaccaatc caggtcacca
    28861 tccgcagtaa cagaatgcgt gtgcgtaaac cttgctggca tttaaaatga atgctcaaca
    28921 gtaaagtgaa catgacgatc actttagtaa acggtaccag aatactggcg ataaacacga
    28981 ttcctgcaac cgcaatgttg ctacttgcca gcgacataat tccggaaaga attgtatctt
    29041 cctgccgtcc tccgttcaga taaatgatag aaataggcaa caggttggca ggtaacaata
    29101 aaacgattga cgctaacagc gccgcccagc atttttgcag actatgacgg cgacgcaggc
    29161 gtagcggaat atgacaacgc gggcagcgac cacgctgatc aggatagccg gtaaaatggc
    29221 acccaagaca gacacgaagt ttctcgtccc tacgcgtagc ggggcgctgc ggataaaatc
    29281 gctcccacag ctcctcgaca ttaagatgtg acaacgtcac cgtcgttaaa atcaccaacg
    29341 ccacaaaaga gaacaagccg acacccgcct ggatatgggc ataatcctgt acctttatag
    29401 aagcaacgcc aatgccgacc aggtagatat cgagcattac ccactcttta agtcgctcaa
    29461 gcatcagcag taccgggcgt agattcattc ccagtcggtt accaaaccat aaataagcta
    29521 tggaggtcac cagaatgagc ggggcaccaa taacgcagaa aaagaccatc gaccccgtta
    29581 tcgcatcgcc ctgtttggtc atttgccaga tgccttgcat aacgttggcg tcaatacgaa
    29641 tgcctaacag ccagatatgc aacagcggtt cgccccaggc aaacggcatc agcaacagca
    29701 tggtgaaggc cattgccgcc aggcgcgtta gcgaccagtc gcgcccgtca cgaatttttg
    29761 cctgacagcg cggacaatag gcactttgat gagaatttat ctcgggcagg ctaaacagca
    29821 tgtcacattg cgggcaacgt tggtaatcac cacgcggcag ttcctcgccg attgccctca
    29881 ctgttatctt ttttgtcggc gtaatttgtg gtgtgttaag agccatgacg tgccaaatgt
    29941 aataagaatt aacattaatc ttaacccatg atgggaatga tcttgagcat taacgcattt
    30001 aatgcttatt ttaataggct ggcagttcca ggtaagacaa ccaagtgatt atataatgaa
    30061 caaaacagaa ttttacgcgg atttaaatcg cgactttaac gcgctgatgg cgggagaaac
    30121 cagttttctg gcaacgcttg cgaacaccag tgcgttgtta tatgagcgtc tcactgacgt
    30181 aaactgggca ggtttttatt tgcttgagga cgatacactg gtgctcggac catttcaggg
    30241 caaaattgcc tgtgtccgga tacccgtcgg gcgtggcgtg tgcggcactg cggttgcccg
    30301 caatcaagtg cagcgtatcg aggatgttca tgcgtttgac gggcatattg cctgtgatgc
    30361 ggcgagtaat tctgaaattg ttctgccgct ggtggtgaaa aatcagatta ttggtgttct
    30421 cgacatcgat agtaccgtct tcggtcgctt tacagacgag gacgagcaag gcttacgtca
    30481 gcttgtggca cagcttgaaa aagtgcttgc aacgacggat tacaaaaaat tctttgcgag
    30541 cgtcgcagga taatcaacgg ataacgtagc atttactgat agcgtcatta taatgacgcc
    30601 tgttcatgcc tgcgcttgtt ggctacgtcc gttgtaatca ggaaatttca tggaaaatca
    30661 acctaagttg aatagcagta aagaagtaat cgcgtttctg gccgaacgtt ttccccactg
    30721 tttcagtgcg gaaggtgaag cgcgtccgct gaaaatcggt atttttcagg atttggtcga
    30781 tcgtgttgcc ggggaaatga acctgagcaa aacgcaatta cgatccgctt tacgtctcta
    30841 cacttcgagc tggcgttacc tttacggtgt taaacccggc gcaacgcgtg tcgatcttga
    30901 cggcaaccca tgcggtgagc tggacgagca acatgtagag catgctcgca agcagcttga
    30961 agaagcgaaa gcgcgtgttc aggcacagcg tgctgaacag caagcgaaaa aacgcgaagc
    31021 tgccgcagct gctggtgaga aagaagacgc accgcgtcgc gaacgcaagc cacgtccgac
    31081 cacgccacgc cgcaaagaag gcgctgaacg taaacctcgt gcgcaaaagc cggtagagaa
    31141 agcgccaaaa acagcaaaag cacctcgcga agaacagcac accccagttt ctgacatttc
    31201 agctctgact gtcggacaag ccctgaaggt gaaagcgggt caaaacgcga tggatgccac
    31261 cgtattagaa atcaccaaag acggcgtccg cgtccagctg aattcgggta tgtctttgat
    31321 tgtgcgcgca gaacacctgg tgttctgaaa cggaggccgg gccaggcatg aacatgtttt
    31381 ttaggcttac cgcgttagct ggcctgcttg caatagcagg ccagaccttc gctgtagaag
    31441 atatcacgcg tgctgatcaa attccggtat taaaggaaga gacgcagcat gcgacggtga
    31501 gtgagcgcgt aacgtcgcgc ttcacccgtt ctcattatcg ccagttcgac ctcgatcagg
    31561 cattttcggc caaaatcttt gaccgctacc tgaatctgct cgattacagc cacaacgtgc
    31621 tgctggcaag cgatgttgaa cagttcgcga aaaagaaaac cgagttaggc gatgaactgc
    31681 gttcaggcaa actcgacgtt ttctacgatc tctacaatct ggcgcaaaag cgtcgttttg
    31741 aacgttacca atacgctttg tcggtactgg aaaagccgat ggatttcacc ggcaacgaca
    31801 cttataacct tgaccgcagc aaagcgccct ggccgaaaaa cgaggctgag ttgaacgcgc
    31861 tgtgggacag taaagtcaaa ttcgacgagt taagcctgaa gctggcagga aaaacggata
    31921 aagaaattcg tgaaaccctg actcgccgct acaaatttgc cattcgtcgt ctggcgcaaa
    31981 ccaacagcga agatgttttc tcgctggcaa tgacggcgtt tgcgcgtgaa atcgacccgc
    32041 ataccaacta tctttccccg cgtaataccg aacagttcaa cactgaaatg agtttgtcgc
    32101 tggaaggtat tggcgcagtg ctgcaaatgg atgatgacta caccgttatc aattcgatgg
    32161 tggcaggtgg tccggcagcg aagagtaaag ctatcagcgt tggtgacaaa attgtcggtg
    32221 ttggtcaaac aggcaagccg atggttgacg tgattggctg gcgtcttgat gatgtggttg
    32281 ccttaattaa agggccgaag ggcagtaaag ttcgtctgga aattttacct gctggtaaag
    32341 ggaccaagac ccgcactgta acattgaccc gtgaacgtat tcgtctcgaa gaccgcgcgg
    32401 ttaaaatgtc ggtgaagacc gtcggtaaag agaaagtcgg cgtgctggat attcctggct
    32461 tctatgtggg tttgacagac gatgtcaaag tgcaactgca gaaactggaa aaacagaatg
    32521 tcagcagtgt gatcatcgat ctgcgtagca atggtggtgg ggcgctgacc gaagcggtat
    32581 cgctctcagg tctgtttatt cctgcgggtc ctattgttca ggtccgtgat aacaacggta
    32641 aagttcgtga agacagcgat accgacggac aggttttcta taaaggcccg ctggtggtac
    32701 tggttgaccg cttcagtgct tcggcttcag aaatctttgc cgcggcaatg caggattacg
    32761 gtcgtgcact ggttgtgggt gaaccgacgt ttggtaaagg caccgttcag caataccgtt
    32821 cattgaaccg tatttacgat cagatgttac gtcctgaatg gccagcgctg ggttctgtgc
    32881 agtacacgat ccagaaattc tatcgcgtta acggcggcag tacgcaacgt aaaggcgtaa
    32941 cgccagacat catcatgccg acgggtaatg aagaaacgga aacgggtgag aaattcgaag
    33001 ataacgcgct gccgtgggat agcattgatg ccgcgactta tgtgaaatca ggcgatttaa
    33061 cggcctttga accggagcta ctgaaggaac ataatgcgcg tatcgcgaaa gatcctgagt
    33121 tccagaacat catgaaggat atcgcgcgct tcaacgctat gaaggacaag cgcaatatcg
    33181 tttctctgaa ttacgctgtg cgtgagaaag agaataatga agatgatgcg acgcgtttgg
    33241 cgcgtttgaa cgaacgcttt aaacgcgaag gtaaaccgga gttgaagaaa ctggatgatc
    33301 taccgaaaga ttaccaggag ccggatcctt atctggatga gacggtgaat atcgcactcg
    33361 atctggcgaa gcttgaaaaa gccagacccg cggaacaacc cgcacccgtc aagtaatatc
    33421 aatcaggcac aagaaattgt gcctgatttt ttaacagcgg caagatgccg taaatcagat
    33481 gctacaaaat gtaaagttgt gtctttctgg tgacttacgc actatccaga cttgaaaata
    33541 gtcgcgtaac ccatacgatg tgggtatcgc atattgcgtt ttgttaaact gaggtaaaaa
    33601 gaaaattatg atgcgaatcg cgctcttcct gctaacgaac ctggccgtaa tggtcgtttt
    33661 cgggctggta ctgagcctga cagggataca gtcgagcagc gttcaggggc tgatgatcat
    33721 ggccttgctg ttcggttttg gtggttcctt cgtttcgctt ctgatgtcca aatggatggc
    33781 attacgatct gttggcgggg aagtgatcga gcaaccgcgt aacgaaaggg aacgttggct
    33841 ggtcaatact gtagcaaccc aggctcgtca ggcggggatc gctatgccgc aagtggctat
    33901 ctaccatgcg ccggacatca acgcttttgc aaccggtgcg cgccgtgatg cctctctggt
    33961 tgctgtcagc accggtttgc tgcagaacat gagcccggat gaagccgagg cggtaattgc
    34021 tcacgaaatc agccacatcg ccaatggtga tatggtcacc atgacgctga ttcagggcgt
    34081 ggtgaacacc ttcgttatct ttatttcccg tatcctggcg cagcttgccg cgggttttat
    34141 gggcggaaat cgtgatgaag gtgaagagag caacggcaac ccgctgatct actttgcggt
    34201 tgcaacggtt ctggaactgg tgtttggtat tctggcgagc attatcacca tgtggttctc
    34261 gcgtcatcgt gaattccatg ctgatgccgg ttcggcaaaa ctggttggtc gcgagaaaat
    34321 gattgccgcg ctgcagcgcc tgaaaaccag ctatgaaccg caagaagcaa ccagcatgat
    34381 ggctttctgc attaacggta agtcgaaatc gctcagtgag ttgtttatga ctcacccgcc
    34441 gctggataaa cgtattgaag ctctgcgtac gggtgaatac ctgaagtaat atgaagtaaa
    34501 tactaaaaag cgcgtcctgc tcgacgcgct ttttttatgc cctggatctt ggctgagtga
    34561 tacgtaaccc actgatacag gcagcaatca ctgccagaat agccgcagcc atcagcgaga
    34621 cgtgtgtacc attatctcca aactgattta gcatcagcgc caccagcgcc gcgccgctac
    34681 tctgacccag tagacgagcc gttcctaaca tgccactggc tccaccgcta cgttcgcgag
    34741 gcgcggaggt aataatggtg tggttattgg gtgactggaa caagccaaat ccagcgccac
    34801 ataagatcat cggccagata atattgatat ccgcaggtga cgcgggcagc agaaccaggg
    34861 aaaaaagccc cgcagccatg atgaataatc ctaaagcacc cagcaatcct gcatgtacgc
    34921 gttcaatcaa atagcctgcc aacggagcca tcaccatcgt tgctaacggc cacggtgtca
    34981 gaagtaaacc tgtttcgact tcactacgcc cgagcacggt ttgcaggtaa aagggcaggg
    35041 aaaccattgc cagcatttgt gcgcagaaag agcaaacaga tgtgcaaata gaaagtgaaa
    35101 acagcgggat acgcagtaaa tccaccggta gcagcggtac tggaagagaa agctggcggc
    35161 gaataaagaa aataccaacg acaaccatta caaccagttc cgcagcaatt aacgtcagcg
    35221 attgcccctg agcgaaacca ctcaacgcag tgataagcag gccgaaggtt aacgcgttca
    35281 tcacggcgct gggcaggtcg aaacggggtt tactggcgcg agaaccatta ggtggcagaa
    35341 aacgcatcgc cagaagcagg gcgataatac ccaacggtac gttgattaaa aataaccatt
    35401 tccaggatgc gatggagagg attgctgcag caattgtcgg cccggcagca gaagagacgg
    35461 caacaataaa cgagtttatg cccatccctc tacccagaaa acgttgtgga tagatcaggc
    35521 ggataagggc ggtattaacg ctcatcaacg ctgcaccgcc gaaaccttgt atgacacgcg
    35581 caagggtgag tatttgcagc gaatcagaaa gggcgcagaa cagtgaagac aacagaaaaa
    35641 cgaccagacc gcatttataa atacgtcgat agccaaacat atcacccaga aacgaaaacg
    35701 agagcaggga gatgacaatg gcgatttgat aggcgttcac tacccagatg gaactggctg
    35761 gcgtggcatg aaggtccgtg gcgattgttg gcagggcgac gttggcgatt gcgccgtcaa
    35821 ggacagccat cgaaatacca atcacaatgg ttaatatcgc accgtatcgc tggggcaatg
    35881 gcaggccgtc ggcctgaact tttggcatag gaattttaaa tctttggtga ataatcaaca
    35941 ggggtatgat tctagcactg atattcacgg tggatgtcgc agaattgtaa ctaaccggca
    36001 aaagatggat tgcgaccacc ctggtgcgaa attataataa aaaacagttc tgatttttat
    36061 aaaacactcg caatgaggtg ataaatggct aacgcagatc tggataaaca gcctgattct
    36121 gtatcttccg tgctaaaagt ttttggcatt ttgcaggcgc tgggtgaaga gcgcgaaata
    36181 gggataaccg agctgtcgca gcgcgtcatg atgtcaaaaa gcaccgttta tcgcttttta
    36241 cagaccatga aaaccttagg ttatgtggcg caggaagggg agtcggagaa atattccctg
    36301 accctgaaat tgtttgaact gggcgctcgc gcgttacaaa acgtcgattt aattcgtagc
    36361 gcagatatcc agatgcgtga gatctcccgc ctgaccaaag aaactatcca cctcggcgca
    36421 ctggacgaag acagtattgt ttacattcac aaaattgact ctatgtacaa tttgcgcatg
    36481 tattcacgga ttgggcgtcg taatccactg tacagcaccg cgattggtaa ggtactgctg
    36541 gcatggcgcg atcgcgatga agtgaagcaa attcttgagg gcgtggagta taaacgcagt
    36601 accgagcgga ccatcaccag tacagaagcg ttattaccgg ttctggacca ggtgcgcgag
    36661 caggggtatg gcgaagataa cgaagagcag gaagaagggc tgcgatgcat tgcggttccg
    36721 gtatttgatc gctttggcgt ggtcattgcc ggtttgagca tctccttccc gacgttgcgt
    36781 ttctctgaag agcgtttaca ggaatatgtc gcaatgttgc ataccgcagc gcgcaaaatt
    36841 tctgcccaaa tgggttatca cgactatccg ttctgatgag agtaagaacc tgtcggaata
    36901 tcaaacagac aggttcttta tttagcatga gaaaaataaa gttgaaggtg gcgttagatt
    36961 aaacgcagtt gatataagaa tattttactc atgagtgaga atctggttat ttattacact
    37021 taaccattat cgaccacgat attgcttttg cgtaacagcg ggcaatctgt tatccccaaa
    37081 aaaccacttt tagtgtgcaa gtattgtacc gtgctggtgc ctctggcagt cagataggta
    37141 cattgcaaac ctaattctgc ggcattctct ttgcttccaa tcaaaacgcc atatccgctg
    37201 agtaataatc ctatccatac cagtgctatc agcataactg tgcgaatgat gaatcgcatt
    37261 acaacctctt ctctttttta tgttcgctta atcgtagcgg caatatgcgc tgaagcaagc
    37321 gactcattcc gaaaaagcac gaatatcgac atagttagac gctgtttaac taacgcatgg
    37381 tagtttaatg acataaggta ggtgaaacgg agattggagt gaaaaagttt cgatgggtcg
    37441 ttttggttgt cgtggtgttg gcttgcttgc tgctttgggc gcaggtattc aacatgatgt
    37501 gcgatcagga tgtacaattt ttcagcggaa tttgtgccat taaccagttt atcccgtggt
    37561 gatatcattt tttcagcact agattttctt cctctccagc agtggtagaa tagcgcctct
    37621 actcagaggt ggtgaaatga acgaagttgt aaattcaggc gtgatgaaca ttgcgtcttt
    37681 ggttgtatcg gtggtggttc ttcttatcgg gctcatcttg tggtttttta ttaatcgtgc
    37741 cagttctcgg actaacgaac agattgaact gcttgaggcg ttgctggatc agcaaaaacg
    37801 tcaaaatgca ctgttacgtc gtttgtgcga agcaaacgaa ccagagaaag cagataaaaa
    37861 gactattgag agtcaaaaat cggttgaaga cgaagatatt attcgcctgg tcgccgaacg
    37921 ataatccttc ctcaatcttg agtaaccccg tcgttgaagg gttactcaat tcatcctgat
    37981 tatacgcact gtcatggtgc aactgcttca aattaaaact ctggtctgac ttcaaataat
    38041 tat
//
LOCUS       DABSYT020000038        37237 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.038, whole genome shotgun sequence.
ACCESSION   DABSYT020000038 DABSYT020000000
VERSION     DABSYT020000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.038"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..723
                     /gene="ydeO"
                     /locus_tag="ID725_RS11580"
                     /old_locus_tag="pgaptmp_002316"
     CDS             <1..723
                     /gene="ydeO"
                     /locus_tag="ID725_RS11580"
                     /old_locus_tag="pgaptmp_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HGV7850908.1"
                     /translation="FNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENT
                     IKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCL
                     SIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQ
                     EQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVS
                     KEYRCQRHTGMNTGNTMNALAI"
     gene            1127..2809
                     /gene="ydeN"
                     /locus_tag="ID725_RS11585"
                     /old_locus_tag="pgaptmp_002317"
                     /pseudo
     CDS             1127..2809
                     /gene="ydeN"
                     /locus_tag="ID725_RS11585"
                     /old_locus_tag="pgaptmp_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            2861..4018
                     /gene="ydeM"
                     /locus_tag="ID725_RS11590"
                     /old_locus_tag="pgaptmp_002318"
     CDS             2861..4018
                     /gene="ydeM"
                     /locus_tag="ID725_RS11590"
                     /old_locus_tag="pgaptmp_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGV7850909.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3984..4106)
                     /locus_tag="ID725_RS11595"
                     /old_locus_tag="pgaptmp_002319"
     CDS             complement(3984..4106)
                     /locus_tag="ID725_RS11595"
                     /old_locus_tag="pgaptmp_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850910.1"
                     /translation="MTKHSTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            4309..5994
                     /gene="yddA"
                     /locus_tag="ID725_RS11600"
                     /old_locus_tag="pgaptmp_002320"
     CDS             4309..5994
                     /gene="yddA"
                     /locus_tag="ID725_RS11600"
                     /old_locus_tag="pgaptmp_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGV7850911.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            6032..8404
                     /gene="yddB"
                     /locus_tag="ID725_RS11605"
                     /old_locus_tag="pgaptmp_002321"
     CDS             6032..8404
                     /gene="yddB"
                     /locus_tag="ID725_RS11605"
                     /old_locus_tag="pgaptmp_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGV7850912.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            8461..11244
                     /gene="pqqL"
                     /locus_tag="ID725_RS11610"
                     /old_locus_tag="pgaptmp_002322"
     CDS             8461..11244
                     /gene="pqqL"
                     /locus_tag="ID725_RS11610"
                     /old_locus_tag="pgaptmp_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGV7850913.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            11605..13005
                     /gene="gadB"
                     /locus_tag="ID725_RS11615"
                     /old_locus_tag="pgaptmp_002323"
     CDS             11605..13005
                     /gene="gadB"
                     /locus_tag="ID725_RS11615"
                     /old_locus_tag="pgaptmp_002323"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGV7850914.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKHMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            13161..14696
                     /gene="gadC"
                     /locus_tag="ID725_RS11620"
                     /old_locus_tag="pgaptmp_002324"
     CDS             13161..14696
                     /gene="gadC"
                     /locus_tag="ID725_RS11620"
                     /old_locus_tag="pgaptmp_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGV7850915.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            14827..16146
                     /gene="digH"
                     /locus_tag="ID725_RS11625"
                     /old_locus_tag="pgaptmp_002325"
     CDS             14827..16146
                     /gene="digH"
                     /locus_tag="ID725_RS11625"
                     /old_locus_tag="pgaptmp_002325"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGV7850916.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            16523..17905
                     /gene="dosC"
                     /locus_tag="ID725_RS11630"
                     /old_locus_tag="pgaptmp_002326"
     CDS             16523..17905
                     /gene="dosC"
                     /locus_tag="ID725_RS11630"
                     /old_locus_tag="pgaptmp_002326"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGV7850917.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            17930..20319
                     /gene="dosP"
                     /locus_tag="ID725_RS11635"
                     /old_locus_tag="pgaptmp_002327"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             17930..20319
                     /gene="dosP"
                     /locus_tag="ID725_RS11635"
                     /old_locus_tag="pgaptmp_002327"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            20577..21158
                     /gene="ddpX"
                     /locus_tag="ID725_RS11640"
                     /old_locus_tag="pgaptmp_002328"
     CDS             20577..21158
                     /gene="ddpX"
                     /locus_tag="ID725_RS11640"
                     /old_locus_tag="pgaptmp_002328"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGV7850918.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            21172..22722
                     /gene="ddpA"
                     /locus_tag="ID725_RS11645"
                     /old_locus_tag="pgaptmp_002329"
     CDS             21172..22722
                     /gene="ddpA"
                     /locus_tag="ID725_RS11645"
                     /old_locus_tag="pgaptmp_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGV7850919.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            22724..23746
                     /gene="ddpB"
                     /locus_tag="ID725_RS11650"
                     /old_locus_tag="pgaptmp_002330"
     CDS             22724..23746
                     /gene="ddpB"
                     /locus_tag="ID725_RS11650"
                     /old_locus_tag="pgaptmp_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7850920.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            23743..24639
                     /gene="ddpC"
                     /locus_tag="ID725_RS11655"
                     /old_locus_tag="pgaptmp_002331"
     CDS             23743..24639
                     /gene="ddpC"
                     /locus_tag="ID725_RS11655"
                     /old_locus_tag="pgaptmp_002331"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGV7850921.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQV
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            24636..25622
                     /gene="ddpD"
                     /locus_tag="ID725_RS11660"
                     /old_locus_tag="pgaptmp_002332"
     CDS             24636..25622
                     /gene="ddpD"
                     /locus_tag="ID725_RS11660"
                     /old_locus_tag="pgaptmp_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGV7850922.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            25615..26541
                     /gene="ddpF"
                     /locus_tag="ID725_RS11665"
                     /old_locus_tag="pgaptmp_002333"
     CDS             25615..26541
                     /gene="ddpF"
                     /locus_tag="ID725_RS11665"
                     /old_locus_tag="pgaptmp_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGV7850923.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAITRALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(26597..27028)
                     /gene="osmC"
                     /locus_tag="ID725_RS11670"
                     /old_locus_tag="pgaptmp_002334"
     CDS             complement(26597..27028)
                     /gene="osmC"
                     /locus_tag="ID725_RS11670"
                     /old_locus_tag="pgaptmp_002334"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGV7850924.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            27373..27588
                     /gene="bdm"
                     /locus_tag="ID725_RS11675"
                     /old_locus_tag="pgaptmp_002335"
     CDS             27373..27588
                     /gene="bdm"
                     /locus_tag="ID725_RS11675"
                     /old_locus_tag="pgaptmp_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGV7850925.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            27690..27827
                     /gene="sra"
                     /locus_tag="ID725_RS11680"
                     /old_locus_tag="pgaptmp_002336"
     CDS             27690..27827
                     /gene="sra"
                     /locus_tag="ID725_RS11680"
                     /old_locus_tag="pgaptmp_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGV7850926.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            27984..29681
                     /gene="maeA"
                     /locus_tag="ID725_RS11685"
                     /old_locus_tag="pgaptmp_002337"
     CDS             27984..29681
                     /gene="maeA"
                     /locus_tag="ID725_RS11685"
                     /old_locus_tag="pgaptmp_002337"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGV7850927.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            29815..30825
                     /gene="adhP"
                     /locus_tag="ID725_RS11690"
                     /old_locus_tag="pgaptmp_002338"
     CDS             29815..30825
                     /gene="adhP"
                     /locus_tag="ID725_RS11690"
                     /old_locus_tag="pgaptmp_002338"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGV7850928.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            30971..31255
                     /gene="yddM"
                     /locus_tag="ID725_RS11695"
                     /old_locus_tag="pgaptmp_002339"
     CDS             30971..31255
                     /gene="yddM"
                     /locus_tag="ID725_RS11695"
                     /old_locus_tag="pgaptmp_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGV7850929.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(31483..32136)
                     /gene="fdnI"
                     /locus_tag="ID725_RS11700"
                     /old_locus_tag="pgaptmp_002340"
     CDS             complement(31483..32136)
                     /gene="fdnI"
                     /locus_tag="ID725_RS11700"
                     /old_locus_tag="pgaptmp_002340"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGV7850930.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(32129..33013)
                     /gene="fdnH"
                     /locus_tag="ID725_RS11705"
                     /old_locus_tag="pgaptmp_002341"
     CDS             complement(32129..33013)
                     /gene="fdnH"
                     /locus_tag="ID725_RS11705"
                     /old_locus_tag="pgaptmp_002341"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGV7850931.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(33026..36073)
                     /gene="fdnG"
                     /locus_tag="ID725_RS11710"
                     /old_locus_tag="pgaptmp_002342"
     CDS             complement(33026..36073)
                     /gene="fdnG"
                     /locus_tag="ID725_RS11710"
                     /old_locus_tag="pgaptmp_002342"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(35486..35488),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGV7850932.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            36305..37186
                     /gene="yddG"
                     /locus_tag="ID725_RS11715"
                     /old_locus_tag="pgaptmp_002343"
     CDS             36305..37186
                     /gene="yddG"
                     /locus_tag="ID725_RS11715"
                     /old_locus_tag="pgaptmp_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGV7850933.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         9693 a         9066 c         9003 g         9475 t
ORIGIN      
        1 ttcaacgcta acattttaga taaagattac gccttctctg acggcgagat cctgatggta
       61 gataacgctg ttcgtacgca ttttgaacct tatgagcggc attttaaaga aatcggattt
      121 aatgaaaata ccattaaaaa atatctacaa tgcactaaca tccagacagt gacgatgcct
      181 gttcctgcga agtttttacg tgcttcgaat gtaccgactg gattgcttaa tgaaatgatt
      241 gcttatctca actcggaaga acgcaatcat cataattttt cagaactttt gcttttttct
      301 tgcctgtcta tttttgccac atgcaaaggt ttcattacac tattaactaa cggtgtgcta
      361 tccgtttcgg ggaaagtgag aaatattgtc aacatgaagc tggcgcaccc atggaagctg
      421 aaagatattt gtgactgcct gtacatcagt gaaagcctgt tgaagaaaaa actaaagcaa
      481 gagcaaacga cattctcaca gattctttta gatgccagaa tgcagcacgc aaaaaatttg
      541 atacgcgtag aaggttcagt caataaaatt gccgaacaat gtggttatgc cagtacatct
      601 tattttattt atgcgttccg caaacatttc ggcaacagtc caaagagagt ttctaaggag
      661 taccgttgtc aaaggcacac gggtatgaat acgggcaaca cgatgaatgc tttagctatt
      721 tgattatttg ctaacgagta gtcaaccacg caccctacgt aaaaattaaa aatggggcag
      781 tcattccctg ccccgcgttg tttttaggcg atatatttat tgaaataaat aagtgacatt
      841 catcacatat ttatgcattt gcataacttg ttgcatgatt atttatgatc tcaattcggc
      901 attttgttag taaaatacaa taatttatta aatatcaata gattaattgt ttatcgtcga
      961 gaaattactt agtagaacaa aaagtaatgt caacgcttta tggactgtta tttccctttt
     1021 tttagctaaa tctgctatct ctttatgtga ctaacttcac ttacatccac ttatttctct
     1081 tcgtaaaatt actttggaat taagtacaat aagaagagga acatttatga agtctgcatt
     1141 aaagaaaagt gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt
     1201 tgctgctcat gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc
     1261 agactttacg ccgactgaat acagtaccaa aggaaagcca aatattatcg tactgaccat
     1321 ggatgatctt ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat
     1381 ggaaaatcgt gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc
     1441 gcaaaaatca acgccgacgc tcctttcatt aatggatgaa ggcgtacgtt ttactaacgg
     1501 ctatgtggcg cacggtgttt ccggcccctc ccgcgccgca ataatgaccg gtcgagcgcc
     1561 cgcccgcttt ggtgtctatt ccaatactga tgctcaggat ggtattccgc taacagaaac
     1621 tttcttgcct gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca
     1681 cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa caaacacgtg actatcatga
     1741 caacttcacc acattttctg cggaagaatg gcaaccgcaa aaccgtggct ttgattactt
     1801 tatgggattc cacgctgcag gaacggcata ttacaactcc ccttcactgt tcaagaatcg
     1861 tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt
     1921 tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc
     1981 gcacctgcca aatgataatc ctgcaccgga gcaatatcag aagcaattta ataccggtag
     2041 tcaaacagca gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat
     2101 tctcgaacaa ctgaagaaaa acggacagta tgacaataca attattctct ttacctccga
     2161 taatggtgcg gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag
     2221 tcagacctat cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca
     2281 acccggtaat tatgacaagc tgatttccgc aatggatttc tacccgacag ctcttgatgc
     2341 agccgatatc agcattccaa aagaccttaa gctggatggc gtttccttgc tgccctggtt
     2401 gcaagataag aaacaaggcg agccacataa aaatctgacc tggataacct cttattctca
     2461 ctggtttgat gaggaaaata ttccattctg ggataactac cacaaatttg tccgtcatca
     2521 gtcagacgat tacccgcata accccaacac tgaggactta agccaattct cttatacggt
     2581 gagaaataac gattattagc ttgtctatac agtagaaaac aatcagttag gtctctacaa
     2641 actgacggat ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga
     2701 gatgcaaggc gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaggtaaa
     2761 tcaggagaag tttaacaata tcaagaaagc actaagcgaa gcgaaataac taaaccttca
     2821 tgcggcggat ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc
     2881 ctccagtttt caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca
     2941 gtttactcat gaaaaatgga tggatgacag cactctgaaa gagttcatca aacaatatat
     3001 cgcagcgtct ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg
     3061 cctggatttt ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat
     3121 ttttaatgca ttacaaacga atggcatttt attgaataat gaatggtgtg cctttctcaa
     3181 agaacatgag tttctggttg gtatctcgat cgatggaccc caggagctac atgaccgtta
     3241 cagacgcagt aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct
     3301 gaaatcatat caagtagagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta
     3361 ccctcttgag gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga
     3421 attgctcgaa accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg
     3481 tatcattgat ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat
     3541 gcaatgggtt aaaaacgatg tgggtgaaat tttcatccgt cagtttgaaa gctttgtcag
     3601 ccgttttttg gggaatgggc ataccagttg tattttccag gagtcctgca aggataatct
     3661 ggttgttgaa agtaatggag atatttacga atgcgatcat tttgtctatc cacagtacaa
     3721 acttggaaac attaataaat ctgaacttaa aacgatgaac agtgtacaac tgacagcgca
     3781 aaaaaaacgg atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg
     3841 ttgccctaag catcgtatta ctaaagtaaa cgatgagact gtttcctatt tttgcgaagg
     3901 ttataaaatc cttttttcaa ccatggtacc ttatatgaac gccatggtgg agttagccaa
     3961 gaacagagta ccgctttacc acattatgga tgttgcaaaa caaatggaga ataattaata
     4021 acgctctctt ttagctccat tcttagcctc cttcttatga ccttaacaag gcacccgaca
     4081 taaattcctg tcgagtgttt cgtcatcatc atcttccctt gcgaaaatct tggcttacgt
     4141 ggtatgcgtc agggcattag caataccctt gattatcaat agcataacca taaccactga
     4201 aaattaaaaa tttctatata tttcatattg ttaaatgata cttaaataaa tttcatcagt
     4261 aaatcattgc catagataat cattggatgg cacaatcaat tttcgctaat gataaccatt
     4321 cccattacgt tttgtatgtt gatagcaaag tatctctgtc tgctgaagcc cttttggctg
     4381 cgtaagaata ataaaacttc agtgttgtta atcattatta tcctcgccat gatccttggc
     4441 gtggttaaaa ttcaggtctg gttaaacgac tggaataatg attttttcaa tgcgctaagc
     4501 cagaaagaaa ccgataagct ttggcagctt gtcctttggt tccctgccct gctggggatc
     4561 tttgtattaa tatccgtaaa caaaacatgg ttaattaaac tactgactat tcgctggcgc
     4621 gaatggttaa cagattacta ccttaatcgc tggtttgcag ataaaaacta ctacttcacg
     4681 cagatctacg gcgagcataa aaatacagat aaccctgacc aacgtatcgc tgaagacatt
     4741 ctcttactga tcagcaaaac gctcagtctt tcatttggct tcatccagtc acttagcatg
     4801 ctgatcacct ttaccgttat tctttggcaa agcgcgggta tgctctcatt cactgttggt
     4861 ggaacagaat ggaatatcca ggggtatatg gtctataccg ttgtgctcat tgtgatcggt
     4921 ggaactttat ttacccataa ggtgggtaaa cgtattcgtc cgcttaatgt ggaaaaacaa
     4981 cgtagcgaag cgacatttcg aactaatctt gtgcagcata ataagcaggc agaactgatt
     5041 gcgctaagca atgctgagtc tctacaacgc caggagttga gcgacaattt tcataccatt
     5101 aaagagaact ggcatcgttt aatgaatcgt cagcggtggc ttgattactg gcagaatatt
     5161 tattcgcgct cgcttagcgt tcttccctac tttctgttat tgccgcagtt tattagcggg
     5221 cagatcaatc tgggcggact gatgaaatcg cgccaggcat ttatgctggt atcgaacaat
     5281 ctaagctggt ttatttataa atatgatgaa cttgctgaac tggctgcggt tatcgatcgc
     5341 ttgtatgagt tccatcaact caccgaacag cgccctacga ataagcctaa aaattgccaa
     5401 catgcggtac aagtggctga cgcgagtatt cgtactcctg ataataagat catattagag
     5461 aacctgaact ttcatgtttc gccaggcaaa tggctattac tgaaaggtta ctctggtgcg
     5521 ggaaaaacca cactgcttaa aacattatcc cactgctggc cgtggtttaa aggtgatatt
     5581 tcttctcctg ccgacagttg gtatgtgtca caaacaccgt taatcaaaac cggcttactg
     5641 aaagagatta tttgtaaagc acttcccctg cccgtagacg ataaatcatt gagcgaagta
     5701 ctgcatcagg ttggtcttgg gaaattggct gcgcgtattc atgaccacga tcgctgggga
     5761 gatattcttt ccagcggcga aaaacaacgt atcgccctgg cacgattaat tttacgacgt
     5821 ccgaaatgga tatttcttga cgaaactacc tctcatcttg aggaacaaga ggctatccgc
     5881 ttactgcgtc tagtgcgtga aaaactaccc acaagcggcg tcattatggt tacacatcaa
     5941 cccggtgtct ggaacctggc cgatgatatt tgtgacatta gcgcggtttt ataaaaacaa
     6001 taaaagccct gctgtacgga gatataaata aatgaagcga gttcttattc ctggcgtcat
     6061 tttatgtggc gctgatgtgg cgcaggccgt cgatgacaaa aacatgtaca tgcatttttt
     6121 tgaagagatg acggtctatg ctcctgtccc tgtacccgta aacggcaaca cgcattacac
     6181 cagtgaaagc atcgagcgtt taccgaccgg gaatggcaat atcagcgatc tgctgagaac
     6241 caaccctgcg gtacgcatgg attcaacgca aagtacctcg ttgaaccagg gagatattcg
     6301 cccggagaaa atctctattc acggtgcgtc gccctatcag aatgcctatt tgattgacgg
     6361 tattagtgcc actaataacc tgaacccagc gaatgagtcc gatgccagta gtgcaaccaa
     6421 tattagcggg atgtcacagg ggtattatct tgatgtcagc ttactggaca atgtgacgct
     6481 ttatgacagt tttgtgccgg ttgaattcgg tcgcttcaat ggcggggtaa ttgatgcaaa
     6541 gttcaaacgc ttcaacgctg atgatagcag cgtgaaactg ggttatcgca ctacgcgttc
     6601 ggactggtta acatcgcata tcaatgagaa taacaagagc gcatttaatc aaggctcttc
     6661 aggaagtact tattactccc cagattttaa aaagaacttt tataccttgt cgtttaatca
     6721 ggaactcgct gataactttg gcgttaccgc cggtttatcg cgccgccagt ctgatatcac
     6781 ccgcgcggat tatgtttcga atgacggcat tgtcgccggt cgggcacagt ataaaaacgt
     6841 tatcgatact gcattgagca aatttacctg gtttgccagc gaccgcttta cccacgattt
     6901 aaccttaaaa tataccggct ccagccgtga ttataatacc agcaccttcc cgcagtctga
     6961 tcgcgaaatg ggtaataaat cctatggtat ggcatgggat atggatacgc agctcgcatg
     7021 ggccaaacta cgtaccaccg ttggttggga tcatattagt gattataccc gtcacgatca
     7081 tgacatctgg tacaccgaac tttcatgtac atatggtgat attacagggc gttgcacccg
     7141 tggcggatta ggacacattt cccaggctgt agataattac accttcaaaa cacgcctgga
     7201 ctggcaaaaa ttcgccgtgg gtaatgtttc gcatcaaccc tacttcggcg cggaatacat
     7261 ctattccgat gcgtggactg aacgccataa ccagtctgaa tcctatgtga ttaatgctgc
     7321 cggaaagaaa actaaccata ccatttacca taaaggtaaa ggcagcctgg gaattgacaa
     7381 ctacacactg tatatggcgg atcgcattag ctggcgtaat gtgtcattaa tgcccggcgt
     7441 gcggtatgac tatgacaact atctgtcaaa ccacaatatc tccccgcgct ttatgacgga
     7501 atgggatatt tttgctgatc aaacctcaat gattacagca ggttataacc gttactatgg
     7561 cgggaatatt cttgatatgg gattacgtga tatccgcaat agctggacgg aatcggtatc
     7621 aggtaataaa accctgacgc gttatcagga tttgaaaacg ccttataacg atgaactggc
     7681 aatgggattg cagcaaaaaa tcggtaagaa cgttattgcg cgcgcaaact atgtttaccg
     7741 tgaagcgcat gatcaaatca gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat
     7801 tactgaatat aacaacgacg gcaaaaccaa aacgcattcg ttcaacctca gttttgaact
     7861 ggccgaaccc ctgcatatca gccaggtaga tattaacccg caaattgtct ttagctatat
     7921 caagagcaag ggcaacttgt cgttaaacaa tggttatgag gagagcaata ccggtgataa
     7981 ccaggtggtt tataacggta atcttgtctc ttacgatagc gttccagtgg cagattttaa
     8041 taacccatta aagatctcct taaacatgga tttcacgcat caaccgagcg ggttggtgtg
     8101 ggcgaatacg ctggcctggc aagaagcgcg taaagctcgc attatcctgg gtaagacaaa
     8161 tgcgcaatac atcagcgaat attcagatta caagcagtat gttgacgaaa aactggatag
     8221 cagcctgacc tgggacaccc gcttgtcctg gacgccacaa tttctgaaac aacaaaacct
     8281 gacgatcagt gccgatattc tcaatgtact ggatagcaaa accgcggttg atacaacgaa
     8341 taccggtgtg gcgacctacg ccagtggccg tactttctgg cttgatgtca gcatgaaatt
     8401 ttaatagttc ggattttact taaccgggta aattcgcccg gttttcgcat ggagataatt
     8461 atgagaaacc tctgtttctt actgacgtta gtggcaactc tgttgctccc cgggcggctg
     8521 attgccgccg ccttaccgca ggatgaaaag ttaattaccg ggcaactgga caatggcttg
     8581 cgatatatga tttatccgca tgctcatcca aaggatcagg taaatttatg gctgcaaatt
     8641 cataccggtt cattgcagga agaagacaat gagcgcggcg tggctcattt tgtagaacat
     8701 atgatgttta acggcacaaa aacatggccg ggtaataaag tcatcgaaac atttgagtca
     8761 atgggcctgc gttttggtcg cgatgttaat gcctatacca gctatgacga aacggtgtat
     8821 caggtgagtt tgccaactac gcagaaacaa aatctgcaac aagtgatggc aatcttcagt
     8881 gaatggagta atgccgcaac ctttgaaaaa ctcgaagtag acgctgaacg cggcgtaatt
     8941 actgaggaat ggcgtgccca tcaggatgcg aaatggcgca cctctcaggc gcgccgccct
     9001 ttcctgctgg caaatacccg taatttagac cgtgaaccta tcggcctgat ggatactgtc
     9061 gccacggtca caccggcaca attgctccaa ttttatcaac gctggtatca accaaataat
     9121 atgaccttta tcgtggttgg cgatatcgac agtaaagaag cgctggcgct gataaaggat
     9181 aatttaagta agcttccggc taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc
     9241 gaaaaccacc tgcgctttaa tatcattaat gataaagaaa accgggtgaa cggcatcgca
     9301 ctctattatc gcctgccaat ggtacaagtg aacgatgagc aaagctttat cgaacaagct
     9361 gaatggagca tgttagttca gctgttcaat caacgtctgc aggaacgtat acagtcgggc
     9421 gagttgaaga ctatttctgg cggcactgcg cgcagcgtta aaattgcacc cgattatcag
     9481 tcgctgtttt tccgtgtaaa tgcacgagac gataatatgc aggatgctgc gaatgcatta
     9541 atggaagagt tggcaaccat tgatcagcat ggtttttctg ctgaagaact cgatgatgtc
     9601 aaatctaccc gcctcacctg gctgaaaaat gcggttgatc agcaagctga acgtgattta
     9661 cgtatgctga ccagtcgcct ggcatccagc tcattaaata atacgccgtt cttgtcgccg
     9721 gaagagacat atcaactttc gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg
     9781 gaaaaatggc agcagttaag aaagaaccag gacgcatttt gggagcaaat ggtaaacaat
     9841 gaggttgccg ccaaaaaagc attgtctcct gcggctatcc tggcgctgga aaaggagtac
     9901 gccaacaaaa agctggcggc ttacatcttc ccaggcagaa atttatcgtt aacagtagac
     9961 gctgacccac aggcggaaat tagcagcaaa gaaacgctgg ctgagaatct gacatcatta
    10021 acactttcca atggtgcaag ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta
    10081 caaattattg ccgtatcgaa taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt
    10141 atcgcgctgg caaataaagc agttagcgga agcggcgttg gcgaactctc ctcttccagc
    10201 ctgaaacgct ggagtgcgga aaactcggta accatgagca gtaaagtcag tggcatgaat
    10261 acgttgctct ccgttagcgc gcggactaat aaccctgaac ctggttttca gttgattaac
    10321 cagcgaatca cccacagcac gattaacgac aatatttggg catcgctaca aaatgctcaa
    10381 attcaggcat tgaaaacgct cgaccagcgt ccagcggaga aattcgccca gcagatgtat
    10441 gagacgcgct atgctgatga ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt
    10501 actgccgcag atgcgctggc tgccgatcgc cagttgtttt catctccagc ggatatcacg
    10561 tttgtcattg tcggtaatgt cgcagaagac aaactcgtgg cgttaattac gcgttactta
    10621 ggatcaatca aacactctga ttcgccatta gccgcaggta aaccattaac tcgcgcgacg
    10681 gacaacgcat cggttactgt aaaggaacaa aatgaacctg tggcacaggt ttcacagtgg
    10741 aagcgttatg attcccggac acctgttaat ctggcgacgc gtatggcgct cgatgctttt
    10801 aacgtcgcac tggcaaaaga tctacgcgtt aatattcgtg aacaggcatc tggggcatac
    10861 agcgtttctt ctcgcctctc ggttgatcct caggctaaag atatcagtca tttgctggct
    10921 tttacttgtc aaccagaacg acatgatgaa ctgttaacgt tagcgaatga agtgatggtt
    10981 aagcgtttga ctaaagggat cagtgagcaa gaactgaacg aataccagca aaacgttcag
    11041 cgcagcctcg atatccaaca gcgtagcgtt caacaattag cgaacactat tgtaaatagt
    11101 cttattcaat atgacgatcc tgcagcatgg actgagcagg agcaattgtt gaaacaaatg
    11161 acggtagaga atgttaacac tgccgttaaa caatatcttt ctcatccggt aaatacttat
    11221 accggagtat tattgccaaa ataataacag ccccgtcaac acatcgttgg cggggatttt
    11281 agcaatattc gctattttta tgtaataatt ttataaatgc gttcaaaata ataaccgcgt
    11341 actaatagtg atattttaag gtctgatttt tacgtgataa ttcaggaggc acagaatacg
    11401 cataaaaata acagcataaa acaccttact accacccaag agctccctac tatattgttt
    11461 ttcaataaaa aatattattc acgcaatatc tcaccataaa taacattagg attttgttat
    11521 ttaaacacga gtcctttgca cttgcttact ttatcgataa atcctacttt tttaatgcga
    11581 tccaatcatt ttaaggagtt taaaatggat aagaagcaag taacggattt aaggtcggaa
    11641 ctactcgatt cacgttttgg tgcgaagtct atttccacta tcgcagaatc aaaacgtttt
    11701 ccgctgcacg aaatgcgcga cgatgtcgca tttcagatta tcaatgacga attatatctt
    11761 gatggcaacg ctcgtcagaa cctggccact ttctgccaga cctgggacga cgaaaacgtc
    11821 cacaagttga tggatttatc cattaacaaa aactggatcg acaaagaaga atatccgcaa
    11881 tctgcagcca tcgacctgcg ttgcgtaaat atggttgccg atctgtggca tgcgcctgcg
    11941 ccgaaaaatg gtcaggccgt tggcaccaac accattggtt cttccgaggc ctgtatgctc
    12001 ggcgggatgg cgatgaaatg gcgttggcgc aagcatatgg aagctgcagg caaaccaacg
    12061 gataaaccaa acctggtgtg cggcccggtg caaatctgct ggcataaatt cgcccgctac
    12121 tgggatgtgg agctgcgtga gatccctatg cgccccggtc agttgtttat ggatccgaaa
    12181 cgcatgattg aagcctgcga cgaaaatacc atcggcgtgg tgccgacttt cggcgtgacc
    12241 tacaccggta actatgagtt cccgcaaccg ctgcacgatg cgctggataa attccaggcc
    12301 gacaccggta tcgacatcga catgcacatc gacgccgcca gtggtggctt cctggcaccg
    12361 ttcgtcgccc cggatatcgt ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct
    12421 tcaggccaca aattcggtct ggctccgctg ggctgcggct gggttatctg gcgtgatgaa
    12481 gaagcgctgc cgcaggaact ggtgttcaac gttgactacc tcggtggtca gattggtact
    12541 ttcgccatca acttctcccg cccggcgggt caggtaattg cacagtacta tgaattcctg
    12601 cgcctcggtc gtgaaggcta taccaaagta cagaacgcct cttaccaggt tgccgcttat
    12661 ctggcggatg aaatcgccaa actggggccg tatgagttca tctgtaccgg tcgcccggac
    12721 gaaggcattc cggcggtttg cttcaaactg aaagatggtg aagatccggg atacaccctg
    12781 tatgacctct ctgaacgtct gcgtctgcgc ggctggcagg ttccggcctt cactctcggc
    12841 ggtgaagcca ccgacatcgt ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac
    12901 tttgctgaac tgttgctgga agactacaaa gcctccctga aatatctcag tgatcacccg
    12961 aaactgcagg gtattgccca acagaacagc tttaaacata cctgataacg tttaacggta
    13021 acggtgtccc gaaacgaacc cgtttcggga caatttccaa agtctgttca ctggcattag
    13081 caacggaaaa tattgttctg aatacgcttc agaacaaaac aggtgcggtt ccgacaggaa
    13141 taccgtttta gggggataat atggctacat tagtacagac aggtaaagct aagcagctca
    13201 cattactcgg cttttttgcc ataacggcat cgatggtaat ggctgtttat gaatacccta
    13261 ccttcgcaac atcgggcttt tcattagtct tcttcctgct attaggcggg attttatggt
    13321 ttattcccgt gggactttgt gctgcggaaa tggccaccgt cgacggctgg gaagaaggtg
    13381 gtgtcttcgc ctgggtatca aatactctgg ggccgagatg gggatttgca gcgatctcat
    13441 ttggctatct gcaaatcgcc attggtttta ttccgatgct ctatttcgtg ttaggggcac
    13501 tctcctacat cctgaaatgg ccagcgctga atgaagaccc cattaccaaa actattgcag
    13561 cactcatcat tctttgggcg ctggcattaa cgcagtttgg tggcacgaaa tacacggcgc
    13621 gaattgctaa agttggcttc ttcgccggta tcctgttacc tgcatttatt ttgattgcat
    13681 tagcggctat ttatctgcac tccggtgccc ccgttgctat cgaaatggat tcgaagacct
    13741 tcttccctga cttctctaaa gtgggcaccc tggttgtatt tgttgccttc attttgagtt
    13801 atatgggcgt agaagcttcc gcaacccacg tcaatgaaat gagtaaccca gggcgcgact
    13861 atccactggc tatgttactg ctgatggtgg cggcaatctg cttaagctct gttggcggtt
    13921 tgtctattgc gatggtcatt ccgggtaatg aaatcaacct ctccgcaggg gtaatgcaaa
    13981 cctttaccgt tctgatgtcc catgtggcac cggaaattga gtggacggtt cgcgtgatct
    14041 ccgcactgct gttgctgggt gttctggcgg aaatcgcctc ctggattgtt ggtccttctc
    14101 gcgggatgta tgtcacagcg cagaaaaacc tgctgccagc ggcattcgct aaaatgaaca
    14161 aaaatggcgt accggtaacg ctggtcattt cgcagctggt gattacttct atcgcgttga
    14221 tcatcctcac caataccggt ggcggtaaca acatgtcctt cctgatcgca ctggcgctga
    14281 cggtggtgat ttatctgtgt gcttatttca tgctgtttat tggctacatt gtgttggttc
    14341 ttaaacatcc tgacttaaaa cgcacattta atatccctgg tggtaaaggg gtgaaactgg
    14401 tcgtggcaat tgtcggtctg ctgacttcaa ttatggcgtt tattgtttcc ttcctgccgc
    14461 cggataacat ccagggtgac tctaccgata tgtatgttga attactggtc gttagtttcc
    14521 tggtggtact tgccctgccc tttattctct atgctgttca tgatcgtaaa ggcaaagcga
    14581 ataccggcgt cactctggag ccaatcaaca gtcagaacgc accaaaaggt cacttcttcc
    14641 tgcacccgcg tgcacgttca ccacactata ttgtgatgaa tgacaagaaa cactaactat
    14701 gtctgaatgg ttataagata agggagcgat aattcatcgc tccctttttc gtgcttgctg
    14761 tcgtattgac ttaaccggga taagtacgag aatgagcgca catctgttta ccggaaacta
    14821 gcacatatgg atatctgctc ccgaaacaag aaattaacga ttagaagacc agcgatactg
    14881 gttgcactgg cacttttact gtgtagttgt aaaagcacgc ctccagagtc catggtgaca
    14941 ccaccagcgg gttcaaagcc accagccacg acgcaacaat cgtcacaacc gatgcgtggc
    15001 atctggctgg ccacggtgtc tcgcctcgac tggccaccgg tttcctcggt taacattagt
    15061 aaccccacca gccgggcccg tgtacaacaa caggcgatga tcgacaaact ggatcatttg
    15121 caacgtctcg gtataaacac ggtctttttc caggtcaagc cggacggtac cgccctgtgg
    15181 ccatcgaaaa ttttgccgtg gtccgatctt atgaccggta agattggtga aaatccgggt
    15241 tacgatccgc tgcaatttat gctcgacgaa gcccacaagc gtgggatgaa agtacacgcc
    15301 tggtttaacc cctatcgcgt atcggttaat acgaagcccg gtactatcag ggaactgaat
    15361 agcactctgt ctcaacaacc ggcgagcgtc tatgtgcaac accgtgactg gatcagaacg
    15421 tctggcgatc gctttgtcct cgacccgggc atccctgagg ttcaggactg gatcacatca
    15481 atagtcgcag aagtggtttc ccgctatccg gtagatggcg tgcagtttga cgactatttc
    15541 tatactgagt caccgggttc acggctaaat gataacgaaa cgtaccgtaa atacggaggc
    15601 gcatttgcgt caaaagcaga ctggcggcgc aacaatactc agcagttaat tgcaaaggta
    15661 tcgcacacca ttaaaagcat taagccggga gtcgaatttg gtgttagccc ggcaggcgtg
    15721 tggcgtaacc gatcacacga tccgctcggt tccgataccc gaggcgcggc agcctatgac
    15781 gaatcctacg ctgacactcg tcgatgggtg gaacaaggat tgctggatta cattgctccc
    15841 caaatttact ggccgttctc acggagtgcc gcgcgttatg acgtgttggc aaaatggtgg
    15901 gcggatgtcg ttaaaccgac caggacccgc ctgtatatcg gtatcgcctt ctataaagtg
    15961 ggtgaacctt caaagataga gccagactgg atgattaacg gcggcgtacc ggagctgaaa
    16021 aagcagctcg atcttaacga tgcggtacca gaaattagcg gcaccatctt gttccgtgag
    16081 gactatctga ataaaccgca gactcaacaa gcggtcagct atctgcaaag tcgttggggc
    16141 agttaaactc ccattcttac tttgtaacaa gccggtgctt tacccactgg cttgttaccc
    16201 tcttgaacta tcccaccagc aatcatgctt actaaagtca aattaaccac gcaattaacc
    16261 acgcttagtt aaacagccat aaatccccta ataaaaatgt atgaatatga aaaaccatac
    16321 gatattcgca gtcaccgaaa aataattacg atgggctata ctcttctgaa gaacagtagc
    16381 gtagattgaa actgtcctaa gtcgcttatt tcttttcagt atatcttcat atttcaggag
    16441 aatatggtgc agttagtcga tctcgcacgt cgcgtttcat tttgattccg tctgatttcc
    16501 cagccttata agggtgtgaa ttatggagat gtattttaaa agaatgaaag atgagtggac
    16561 cggacttgtc gagcaggcag atccgcccat tcgtgctaaa gccgcggaaa ttgccgttgc
    16621 gcatgctcat tatctgagta tcgagtttta tcgaattgtc cgcatcgacc cgcatgccga
    16681 agaattcttg agtaatgaac aagttgagcg gcagttgaaa agtgcgatgg aacgctggat
    16741 tattaacgtg ctttctgccc aggttgacga tgtcgaaagg ctaatacaaa tccagcatac
    16801 cgtcgcggaa gtgcatgccc gcataggaat tccggtagaa attgtcgaga tggggtttcg
    16861 ggtgctgaaa aagatcctct atccggtaat cttctcttcg gattattctg ccgcagaaaa
    16921 acttcaggtc taccatttct cgattaacag tattgatatc gctatggaag tgatgacccg
    16981 cgcgtttacc tttagtgaca gtagtgcctc aaaagaagat gaaaactatc gtatcttctc
    17041 gttactggaa aacgccgaag aagaaaaaga acggcaaata gcctcaatac tttcatggga
    17101 aatagatatt atctataaaa tcctgctgga ttctgattta ggcagtagtt tgcctttaag
    17161 ccaggctgat tttggcctgt ggtttaacca taaaggtcga cattatttca gcggcattgc
    17221 tgaagtgggc catatctctc gtctgattca ggatttcgac ggtattttca atcaaaccat
    17281 gcgtaacacc aggaatttga ataacagaag tctgcgggtg aaatttttat tacagataag
    17341 aaataccgta tcgcaaatta ttaccttgct gcgtgaattg tttgaagaag tatcgcgcca
    17401 cgaagtcggt atggatgtac tgacgaaatt acttaaccgc cgtttcctgc cgactatctt
    17461 caaacgcgaa attgcccatg ccaaccggac cggtacaccg ctgtcagtgc tgattattga
    17521 cgttgataaa ttcaaagaga tcaacgatac gtggggccat aacactggcg atgaaattct
    17581 gcgtaaagtc tctcaggcct tttatgacaa cgtccgcagt agtgattatg ttttccgcta
    17641 cgggggcgat gaatttatca ttgttttgac tgaagcttct gaaaacgaaa cgttacgtac
    17701 cgcagaacgt attcgcagtc gggtggagaa aaccaaactg aaagccgcaa acggcgaaga
    17761 tattgccctc tcactttcca tcggtgccgc catgtttaat ggtcatcctg actatgagcg
    17821 cctcattcaa atagccgatg aagctctgta tatcgccaaa agacgaggta gaaaccgtgt
    17881 tgaactctgg aaagccagtc tttagatgcg ccaggatgca gaggtaatca tgaagctaac
    17941 cgatgcggat aatgccgccg atggcatttt tttccccgcc cttgagcaaa atatgatggg
    18001 tgcggtgtta attaacgaaa atgatgaagt gatgtttttc aaccccgccg cagagaagct
    18061 ctgggggtac aaacgtgaag aagtcattgg caataacatt gatatgctga ttccgcggga
    18121 tttgcgtcct gcgcatcctg aatacattcg tcacaaccgt gaaggcggta aagcgcgcgt
    18181 cgaggggatg agtcgggagc tgcagctgga gaaaaaagac ggcagtaaaa tctggacccg
    18241 ttttgcgcta tcgaaagtga gcgccgaggg gaaagtttat tacctggcgc tggtacggga
    18301 tgccagcgta gaaatggcgc aaaaagaaca gaccaggcaa ttgattattg ccgttgacca
    18361 tctcgaccga ccggtgattg tcctcgatcc ggaacgccat attgtgcagt gcaatcgcgc
    18421 atttaccgaa atgtttggtt actgcattag cgaagccagc ggtatgcagc ctgatacact
    18481 cctgaacatt cctgaattcc ctgccgataa ccgcattcgt ttacaacagt tgctttggaa
    18541 aaccgcccgc gatcaggacg aatttctgct gttgacgcgc accggtgaaa aaatctggat
    18601 taaagcttct atcagcccgg tttatgacgt gctcgcgcat ctgcagaacc tggtaatgac
    18661 cttctcggat atcaccgaag aacggcaaat ccgccagctt gaaggcaata ttctcgccgc
    18721 catgtgcagc agcccgccat ttcatgaaat gggggaaatc atttgtcgta acatcgaatc
    18781 tgttctcaac gaatcgcatg tttcgctgtt cgcactgcgc aacgggatgc cgatacactg
    18841 ggcgtcatct tcgcacggtg cagaagttca aaatgcgcaa agctggtcag cgaccattcg
    18901 tcagcgtgat ggcgcgcctg cggggatcct gcaaattaaa acctcgtcag gagcagaaac
    18961 cagcgccttt atcgaacgcg tggcagatat cagccagcat atggccgcgc tggcgctgga
    19021 acaggaaaaa agccgtcagc atattgaaca actcatccaa tttgatccga tgaccggtct
    19081 gccaaatcgc aataacctgc acaattacct cgatgacctg gtcgacaaag ccgtctctcc
    19141 agtatcggtg ttgaccatat tcaggatgtg attgatagcc ttggctatgc gtgggccgat
    19201 caggcattgc tggaagtggt caatcgcttt cgtgaaaaac tcaaaccgga tcagtatctc
    19261 tgtcgtatcg aaggtacgca gtttgtcctc gtgagcctgg aaaacgacgt cagtaacatt
    19321 acccagatcg ccgatgagct acggaatgtg gtcagcaagc cgataatgat tgacgataac
    19381 cccttcccgc ttaccttgag tattggcatc agctacgacg tgggtaaaaa tcgcgattac
    19441 ctgctctcca ctgcccataa cgcgatggat tatattcgca agaatggcgg taacggctgg
    19501 cagttcttca gcccggcgat gaacgaaatg gtaaaagagc gtttggtttt aggcgcagcg
    19561 ctgaaagaag cgattagcaa taaccaactg aaactggttt accagccgca aatcttcgca
    19621 gaaacgggtg aactctacgg catcgaagcc cttgctcgct ggcacgatcc ccagcatggt
    19681 catgtgcccc cttcacggtt tattcctctc gcagaagaga ttggtgaaat cgaaaatatt
    19741 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
    19801 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
    19861 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtagaa
    19921 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
    19981 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
    20041 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagttttgt cgatcgttgt
    20101 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
    20161 aatttaaccg tcgtggcgga aggcgtcgaa accaaagagc aatttgagat gctacgaaag
    20221 atccactgtc gcgttattca gggatatttc ttttcccgcc ccctacccgc cgaagaaatt
    20281 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct ctcccgcact
    20341 cgcgggtttt catttaacgt gacactgtca cttaaatact gtgatttcag ctacgattcc
    20401 gggagattcc ttccttaact tcccctcttg caccaaaact gcactacgct gcacaaatgt
    20461 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
    20521 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
    20581 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
    20641 aatacgcctg cgctgataac atcacaggta aagctattta ccagcaagcg cgttgtctgt
    20701 tacacaagga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctatcaggct
    20761 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
    20821 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
    20881 cggcgatcga cctgacgctt cgtgatgagc acgggaacat cctggatatg ggcgcagggt
    20941 tcgatgaaat gcacgagcgc tcccatgcct atcacccttc cgtcccgccc gccgctcagc
    21001 gcaatcgcct gttgctgaat gcgataatga ctggtggcgg ttttgtcggt atctccagcg
    21061 aatggtggca cttcgaatta ccccaggcag cgagttaccc tctgctcgct gatcaattca
    21121 cctgttttat atcccctggc acacagcacg tcagttaatc aggaaactgc catgaagaga
    21181 tcgatatcgt ttcgtcccac attgctcgcg ctcgtccttg ccacaacttt cccggttgcg
    21241 cacgccgccg taccgaaaga tatgctggtg attggtaagg ccgccgatcc acaaaccctc
    21301 gacccggcgg taacaataga taataacgac tggacagtga cctacccgtc ttatcagcga
    21361 ctggttcagt acaaaacgga cggtgataaa ggctcaaccg acgttgaagg cgatctggca
    21421 agtagctgga aagcgtctaa cgatcaaaaa gagtggacgt tcaccctgaa agataatgct
    21481 aaatttgccg atggcacacc tgtcactgcc gaagcagtaa aactctcttt tgagcggtta
    21541 ctaaaaatcg gccaggggcc agcagaagca tttcccaaag atttaaagat tgatgctccc
    21601 gacgaacata cggtgaagtt tacccttagc cagccatttg caccgttcct ctacacgctg
    21661 gcgaatgacg gtgcatccat tatcaatccg gcggtattaa aggaacatgc ggcggatgat
    21721 gcccgcggct tcctcgcgca aaataccgcc ggttccggac catttatgct gaaaagctgg
    21781 caaaaaggtc agcaattagt tctggtgcca aatccgcatt accccggcaa taaaccgaac
    21841 tttaagcgag tatcggtaaa aattatcggt gaaagtgcct cccgtcgcct gcagctctcc
    21901 cgtggcgaca ttgacattgc cgatgcgctg ccggtggatc aactcaacgc cctgaagcag
    21961 gaaaacaaag tcaatgtggc agagtatccg tcactgcgcg tcacctatct gtatctgaat
    22021 aacagcaaag cgccacttaa tcaggcggat ctgcggcggg ccatttcctg gtctaccgat
    22081 tatcagggca tggttaacgg cattctgagt ggtaacggaa aacaaatgcg cggcccgatt
    22141 ccggaaggca tgtggggcta cgatgcgacg gcaatgcaat acaaccatga cgaaacgaaa
    22201 gccaaagccg aatgggataa agtgacgagc aaacccacca gcctgacgtt tctctactcc
    22261 gataacgatc cgaactggga accgatcgct ctggcaacac aatccagcct caacaagctg
    22321 ggcatcaatg tgaagctgga aaagctggcg aacgccacca tgcgcgacag agtgggtaaa
    22381 ggtgattacg acattgcgat tggcaactgg agtccggatt ttgccgaccc gtatatgttt
    22441 atgaattact ggtttgagtc agacaaaaaa ggtctgccgg gtaaccgctc gttctatgaa
    22501 aacagtgagg tcgataagtt actgcgcaat gcgcttgcga ccaccgacca gacgcagcgt
    22561 acccgagact accagcaggc acagaaaatc gtcattgatg acgctgctta tgtgtacctg
    22621 ttccagaaaa actaccaact ggcgatgaac aaagaggtga aaggctttgt gttcaatccc
    22681 atgctggaac aggtcttcaa tatcaatacc atgagtaaat aacgtgacct tctggagtat
    22741 tttacgccaa cgctgctggg ggctggtgct cgtggtggcg ggcgtctgcg tgattacgtt
    22801 tattatctcg catctgatcc ccggcgatcc ggcgcggtta ctggcgggtg accgcgccag
    22861 cgatgctatc gtggaaaata ttcgccagca actgggactg gaccagccac tgtatgtaca
    22921 gttttaccgc tacgtcagcg acctgtttca tggtgacctg ggaacatcca ttcgtactgg
    22981 gcgtccggtg ctggaagagt tgcgtatatt tttcccggcg acgctggagc tggctttttg
    23041 tgccctgctg ctggcactct tgattggcat cccgctgggc atactctctg cagtctggcg
    23101 aaatcgctgg ctggatcatc tggtgcgaat aatggcgatt accggaatct ccacacctgc
    23161 gttctggctt gggctgggcg tcatcgtgct gttttatggt catctgcaaa tacttcccgg
    23221 cggcggaagg cttgatgact ggctggatcc accaacgcac gttaccggct tttatctgct
    23281 cgatgcgttg cttgaaggca acggtgaagt cttcttcaat gcgttgcaac atctcatctt
    23341 accggcatta acgctggcgt ttgttcacct ggggattgtc gcccgccaga tccgctcagc
    23401 gatgctggaa caattgagtg aagactacat tcgtaccgcc cgggccagcg gcttgcccgg
    23461 ctggtatatc gttctatgtt atgcgctacc caatgcgttg atcccttcga ttaccgtatt
    23521 ggggctggcg ctgggcgatt tgttgtatgg cgcagtgctc accgaaaccg tttttgcctg
    23581 gcccggaatg ggggcatggg tagtgacatc aatacaggcg ctcgacttcc cggcagtgat
    23641 gggctttgcc gtcgtggttt catttgctta tgtgctggtc aacctggtgg tggatttgct
    23701 ctatttgtgg attgatccgc gtatcggacg tggaggtggt gaatgatgct aagcgaggaa
    23761 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
    23821 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gctactgatg
    23881 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
    23941 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttc
    24001 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtcgt ggttgccatt
    24061 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
    24121 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
    24181 gcactggctg ccgctctcgg gccgagtttg tttaacgcca tgctggctat tgctattgtg
    24241 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
    24301 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
    24361 ttacgtaact ccctaccgcc gctgatcgtg caggtatcgc tggatatcgg tagcgcgatt
    24421 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
    24481 ggcgcgatgg tagcaaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
    24541 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
    24601 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
    24661 aacaactgca tttgagtttc cccggtttta acggcgacgt tcacgcgctc aacaatgtgt
    24721 ccttgcagat taaccgcggt gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
    24781 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
    24841 ggcagatttc actgctggga gacgatgttc ttaacgcgcg ggaaaaacag cttcgtcagt
    24901 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctc aatccgacac
    24961 gtcgaatagg tcttcagatg atggacgtga tccgccatca tcaaccaata agtcgtcggg
    25021 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat accgtggaag
    25081 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
    25141 tggcattctc ctgcgaaccg caattgatta ttgccgacga accgactacg gcgctggacg
    25201 tcacggtaca gttgcaggta ctgcgtttgc ttaaacataa agctcgcgac agtggaactg
    25261 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
    25321 tgatgtatgc cggaagcgtg attgagagcg gcgtgacggc agacgttatc catcatcccc
    25381 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
    25441 cattacccgc cattccaggg acggtaccaa atctcaccca tttgcctgac ggctgcgctt
    25501 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
    25561 gtggtgacaa caaccagcgc tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
    25621 gacacgttat taacgttacg cgacgtccat atcaatttcc cggcgcgtaa aaactggctt
    25681 ggtaaaacta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
    25741 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
    25801 atgggtatgc tgcaaccgag ccacgggcag tacatccgtt ctggctcaca acgcattatg
    25861 cagatgatgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
    25921 atcacagaac cgctctggat agctaagcat agtagtgaac aacagcggcg agcgttggca
    25981 gaggagctgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
    26041 ttctccggcg ggcagcggca acgcatcgcc attaccagag cactctcttc gcagcctgac
    26101 gtgattgtgc ttgatgagcc aacctctgcg ctggatatct ccgtgcaggc gcagatcctc
    26161 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
    26221 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgtg
    26281 gaattagggg aagcgcaaca ggtgctaacg gctccggcac atccatacac ccgattattg
    26341 ctggattccc tccccgccat tgataaacca ctggaggaag aatgggcatt acgtaaaacg
    26401 gatctgccag gaaaccgcac gttgccacag ggctgttttt tccgcgaacg ttgccccctt
    26461 gcaaccagcg gatgcgaagt ccggcaatca ttaacgacgc gggctgacgg acgtgagatc
    26521 cgttgctggc gggcgctgta gagatgctaa caacgcatca ggcattcatg cgcctgacgc
    26581 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
    26641 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
    26701 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
    26761 ccactttatc cagcgacaca tcggcggtgg tatcaatcga tgttggcgtg aatcccgctt
    26821 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
    26881 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
    26941 tcagcacgcc actctcggtg gatactgttc ccttcccgcg tttgatatcg ctttcccagt
    27001 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
    27061 atagactacg tgagaaaccg cacgaggata agcaggatat tccgaataaa atacggcaaa
    27121 aatattactt ctgcacttaa attaaattcc cacaatagat aaccggaaat atatatctca
    27181 agagagattg catcaatatt aatcaactca tcacaataat aatacctatc aatatcatgg
    27241 ggttaaaata taaaaatcat caaccaggac taatcttaac aacgaagcgg caaatatttg
    27301 ccatgctgaa tgtgctttat aaatctgcga tccgtagcag acactataaa tacacagaca
    27361 cggagaatca ctatgtttac ttattatcag gcagaaaatt caacagcaga acccgctctg
    27421 gttaatgcca ttgagcaagg ccttcgggca gagcacggtg ttgtcactga agatgacatt
    27481 ctcatggagc tgaccaaatg ggtggaagct tctgataacg acatcctcag tgatatctac
    27541 cagcaaacga tcaattatgt ggtcagtggc cagcacccta cgctttaaga tgctatgctt
    27601 gatcggcaac ctaatttagg ggtttagcac gtgtttcttc gctacggcga tgttgtcctt
    27661 aaaactagca acaggattga ggagttaaaa tgaaatcgaa ccgtcaggca cgtcatattc
    27721 ttggactgga ccataaaata tctaaccagc gcaaaatagt taccgaaggt gacaaatcca
    27781 gcgtggtaaa taacccaacc ggcagaaaac gccccgctga aaagtaattc ataaccatca
    27841 gtcctcaatg acgattaaac accattgcct gcgcaatggt gtttttgttt ttatctgctt
    27901 tatacttggg gccgacgccc tggcggtaaa gcaaagacga taaaagcccc ccagggatgg
    27961 atattcaaaa aagagtgagt gacatggaac caaaaacaaa aaaacagcgt tcgctttata
    28021 tcccttacgc tggccctgta ctgctggaat ttccgttgtt gaataaaggc agtgccttca
    28081 gcatggaaga acgccgtaac ttcaacctgc tggggttact gccggaagtg gtcgaaacca
    28141 tcgaagaaca agcggaacga gcatggatcc agtatcaggg attcaaaacc gaaatcgaca
    28201 aacacatcta cctgcgtaac atccaggaca ccaacgaaac cctcttctac cgtctggtaa
    28261 acaatcatct tgatgagatg atgcctgtta tttatacccc aaccgtcggc gcagcctgtg
    28321 agcgtttttc tgagatctac cgccgttcac gcggcgtgtt tatctcttac cagaaccgcc
    28381 acaatatgga cgatattctg caaaacgtgc cgaaccataa tattaaagtg attgtggtga
    28441 ctgacggtga acgtattctg gggcttggtg accagggcat cggcgggatg ggcattccga
    28501 tcggtaaact gtcgctctat accgcctgtg gcggcatcag cccggcgtat acccttccgg
    28561 tggtgctgga tgtcggaacg aacaaccaac agctgcttaa cgatccgctg tatatgggct
    28621 ggcgtaatcc gcgtatcact gacgacgaat actatgaatt cgttgatgaa tttatccagg
    28681 ctgtgaaaca acgctggcca gacgtgctgt tgcagtttga agactttgct caaaaaaatg
    28741 cgatgccgtt acttaaccgc tatcgcaatg aaatttgttc ctttaacgat gacattcagg
    28801 gcaccgcggc ggtaacagtc ggcaccctga tcgcagcaag ccgcgcggca ggtggtcagt
    28861 taagcgagaa aaaaatcgtc ttccttggcg caggttcagc gggatgcggc attgccgaaa
    28921 tgatcatcgc ccagacccag cgcgaaggat taagcgagga agcggcgcgg cagaaagtct
    28981 ttatggtcga tcgctttggc ctgctgaccg acaagatgcc gaacctgctg cctttccaga
    29041 ccaaactggt gcagaagcgc gaaaacctca gtgacaggga taccgacagc gatgtgctgt
    29101 cactgctgga tgtggtgcgc aatgtaaaac cagatattct gatcggcgtc tcaggacaga
    29161 ccgggctgtt tacggaagag atcatccgtg agatgcataa acactgtccg cgtccgatcg
    29221 tgatgccgct gtccaacccg acgtcacgcg tggaagccac accgcaggac attatcgcct
    29281 ggactgaagg taacgcgctg gtcgccacgg gcagtccgtt taatccagtg gtatggaaag
    29341 ataaaatcta ccctatcgcc cagtgtaaca acgcctttat tttcccgggc atcgggctgg
    29401 gtgttattgc ttccggcgcg tcacgtatca ccgatgagat gctgatgtcg gcaagtgaaa
    29461 cgcttgccca gtattcgccg ctggtcctga acggcgaagg tctggtacta ccggaactaa
    29521 aagatattca gaaagtctcc cgcgcaattg cgtttgcggt tggcaaaatg gcgcagcagc
    29581 aaggcgtggc ggtgaaaacg tctgccgaag ctttgcaaca agccattgac gataatttct
    29641 ggcaagccga ataccgcgac taccgccgta cctccatcta agcctgcgcc cggtagtgaa
    29701 ggctaccggg ctatttccct ctcccttttt cagatctcat ccatactggg tagtggcgaa
    29761 taaatctcat ttgcctcacc tgctatgcag aacatcatcc gaaaaggagg aactatgaag
    29821 gctgcagttg ttacgaagga tcatcatgtt gacgttacgg ataaaacact gcgctcactg
    29881 aaacatggcg aagccctgct gaaaatggag tgttgtggtg tatgtcatac cgatcttcat
    29941 gttaagaatg gcgattttgg tgacaaaacc ggcgtaattc tgggccatga agggattggt
    30001 gtggtggcag aagtgggtcc aggtgtcacc tcattaaaac caggcgatcg tgccagcgtg
    30061 gcgtggttct acgaaggatg cggtcattgc gaatactgta acagtggtaa cgaaacgctc
    30121 tgccgttcag ttaaaaatgc cggatacagc gttgatggcg ggatggcgga agagtgcatc
    30181 gtggtcgccg attacgcggt aaaagtgcca gatggtctgg actcggcggc ggccagcagc
    30241 attacctgtg cgggggtcac cacctacaaa gccgttaagc tgtcaaaaat tcgtcccggg
    30301 cagtggattg ctatctacgg tcttggcggt ctgggtaacc tcgccctgca atacgcgaag
    30361 aatgtcttta acgcgaaagt gatcgccatt gatgtcaatg atgagcagtt aaaactggca
    30421 accgaaatgg gtgcagattt agcgattaac tcacgcaccg aagacgccgc caaaattgtg
    30481 caggagaaaa ccggtggcgc tcacgctgcg gtggtaacag cagtagctaa agctgcgttt
    30541 aactcggcag ttgatgctgt ccgtgcaggc ggtcgtgttg tggctgtcgg tctgccgccg
    30601 gagtctatga gcctggatat cccacgtctt gtgctggatg gcattgaggt ggtcggttcg
    30661 ctggtcggca cgcgccagga tctaactgaa gccttccagt ttgccgccga aggtaaagta
    30721 gtgccgaaag tcgccctgcg tccgttagcg gacatcaaca ccatctttac cgagatggaa
    30781 gaaggcaaaa tccgtggccg tatggtgatt gatttccgcc gctaagaggc ctttgctgcg
    30841 actcccatgt tcgggtcgca gcatcgcaca ctctccaaca tgaaatggct aaatggatta
    30901 ttgttgcgta ggaaggaaca ataatgatag tgttcccaga gaaggaacgc tactgagagt
    30961 aaagacacta atgaaaatgg ccaatcatcc ccgcccgggg gacattattc aggaatcact
    31021 ggacgaactt aatgtcagcc tgcgcgagtt tgccagagca atggaaattg ctccctcaac
    31081 ggcaagccga ttgctgaccg gaaaagcagc tttgacgcca gaaatggcaa ttaaactttc
    31141 cgtggtgatc ggcagttcgc cgcaaatgtg gctgaatctg caaaacgcct ggagtctggc
    31201 agaggctgca aaaacggtgg atgtgtcgcg attacgccgt ctggttgcgc aataaaaaat
    31261 ggcgcctttt acagcgccat ttgaaactga tgacaaacgc aacattgcct gatgcgctac
    31321 gcttatcagg cctacacgtt cactgcaatc tattggattt gaacggtttg taggccggat
    31381 aaggcgttca cgccgcatcc ggcatgaaca aagcctacgt tgtctacaat atgaaaatgg
    31441 cgccttttac agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact
    31501 ctcttttttc gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc
    31561 ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc gatcctttca cccagaatgc
    31621 catatacata tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag
    31681 caggctgtag cgaacaacct gcatcgggaa gtactgcgca aagtacggac gccagataat
    31741 caccccggtc accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg
    31801 cccggcgttg tacttaccga catccgccac tttatgctca ttgcctttca atacttcgac
    31861 aatgtttaac agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac
    31921 aaacataaac atcagtgcga cgaaaatcgc aatgccgaag aatgggtgca aaatgcgtcc
    31981 catctgcggc gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat
    32041 cccggacagc gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat
    32101 aaatttggtg cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca
    32161 cttccttatt cgggccaata ccgatgtagt ggaaaatcaa ccctgcaaaa gtggcaataa
    32221 agccagccgc tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct
    32281 tcggatcttt cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca
    32341 taacgtgcgt accaccgacc ccttccgggt tgtagacgcc agcatgttcg taaccacgcg
    32401 ctttcagttt cgccacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt
    32461 ggatagcccc ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat
    32521 cgacgcagag cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa
    32581 acggacaccc ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa
    32641 tcccgttagc gtactgaatg attgcaccgg cagacgggca cgccttcagg cagcccggat
    32701 cttcacagtg catacagcca tctttacgga tcagccactc cagcttgccg ctctgttcgg
    32761 tttcgctaaa gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa
    32821 ccccgacgca gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac
    32881 aggctttaca gccgatacag gtggaaacgt cgataagttt tgcgacttct gctttgtaat
    32941 cacgcacctg agaaggcggc gtgatggagt tagttgcgga ccttttgata atgtcctgcg
    33001 tttccatagc catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat
    33061 attccggcgt ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gctatataac
    33121 ctttacgcgc gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct
    33181 gaccatttac attcagcggt ttcagacgac gcgttaccac agccaccgcg cggataaagc
    33241 cacgcttgct tgagacagtg acacgatcgc cattattaat gcctttcgcc gccgccagcg
    33301 tttcgctgat ttccacaaac tgttccggct gagcaattgc gttgagcaac gcgtgcttgg
    33361 tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc cacatacggg aactgctctt
    33421 ttttacccat ccgcagcgcg tcttgttcat acagacgaac caccgggtta gaaaccacgt
    33481 tcggatgcag cgggttagtt cccagcggcg tttcaattgg ctcgtagtgt tccgggaacg
    33541 gaccttccgc cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcatgataa
    33601 acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc agggatatcg ttacccgtcc
    33661 acttgctgcc gttccactgg atcagcatcc gtttcggatc ccacggttta ccgttgatat
    33721 ctgccgaagc acggttgtag agtacgcgac ggttaagcgg ccacgcccag gcccatccca
    33781 gcgtattccc cagaccggac gggtcggagt tatcgcggtt agccatctgg ttgccctgct
    33841 ctgtccagct accggtgtag atccagcaag aagatgcggt tgtaccgtca tcacgcagat
    33901 gcgcaaagct actcagcaac tgacctttct tcgcaatcag cacgccatta gcatcataga
    33961 gatcttccag cgcatagccg ttgttctctt tagctacttc gtcagattgc ggttcgtgcg
    34021 gctgcttgta gttccagctc atcttcatca gcggttctac gcctttacca ccttcagcct
    34081 ggtacagctc gcgcagatga tggtagatac ccgccagaat ttcgccgtcg ttacgcgctt
    34141 cgcccggcgc gtcctgacct ttccagtgcc actgcaacca gcgaccggag ttagcgatag
    34201 aaccatcttc ttctgcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagacg
    34261 ccggatcgac atcgttcgac tcgccgtggt tctgccagaa ggtagaggtt tcagtcacca
    34321 gcggatcgat aaccaccatg tacttcagct tgctcaggca gctcaccact ttgtttttgt
    34381 ccgggaagga cgcaaccggg ttaaagccct ggcagaaata accggtgact ttgccttcat
    34441 ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcat
    34501 agccccagtt gttctctttc tgcgcggcat cgccatagaa agatttcatc aggctaacga
    34561 agaacttcgg atagttgctc cagtagttca cctgaccagc cagcgtcgct ttcggcgtgt
    34621 tcgcttccag atacgactgc aaatcaacct gtttttctga cggcagcgtc agataacctg
    34681 gcaggctggt agagagcaga cctaagtcag tcagaccctg aatgttggag tgaccacgca
    34741 atgcattcac gccgccaccg gccataccca tgttaccgag cagcaactgg atcatcgcca
    34801 tagtacggat gttctgcgca cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg
    34861 tggttgtgcg atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg
    34921 cttttggcgt accacagatg ttttcaacaa cgtccggcgt gtagcgggaa acgtgctctt
    34981 tcagcaggtt ccacacacag cgcggatgag tcagtgtttc atcgcgtttt gcatagccgt
    35041 tttcatcgaa ctgatagttc caggacgact tatcgtactg gcgtttttca gcgtcgtagc
    35101 cgctgaacag accgtcttcg aaagcaaaat catcacgcac cagcaggctg gcgttggtgt
    35161 aatgcttaac gtattcggcg ttgattttgt tgttttcgat caggtagcgc aaaacgccag
    35221 acaggaacgt aatgtccgta ccggaacgaa taggcgcgta gatatccgcc acagaagcgg
    35281 tacgcgtaaa acggggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca
    35341 tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt accgcccatt accattacga
    35401 cgttagcgtt tttgatatcc acccagtggt tggtcatcgc accgcgacca aatgttggag
    35461 caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc
    35521 cgagggagcg ggcaaatttc tgggtcagca tcccggtttc gttgctggca ccggaagcac
    35581 acagcatacc ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa
    35641 agttagcgtc acggtcagct ttcatcagct tcgcaatacg ggagaatgct tcttcccagc
    35701 taatgcgctg ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt
    35761 cgctgttgac gtaatccagc aaaccggccc ctttcgggca cagcgcccca cggcttaccg
    35821 gatgatccgg gtcaccttca atgtgataaa tcgcttctct ggcgtttttc gcgccatcac
    35881 ccaggctata catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct
    35941 ctttagcgcg taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc
    36001 ccaatgccgc tactgttgtt ccagccatac cgcccgcgca gattttaaaa aattgtctgc
    36061 gactgacgtc cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag
    36121 taaaattctg aatctttttg cgcgaaatca aaaagaacac gtgtttgcca caaaataatc
    36181 cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc
    36241 cagttaaaaa aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc
    36301 gagcatgaca cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac
    36361 gatggtagga ttgattcgcg gtgtcagtga ggggctcggc ccggtcggtg gcgcagctgc
    36421 tatctattca ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcggca
    36481 aatcccgaaa ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct
    36541 ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa
    36601 ctatctgtgg cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa
    36661 ctggctgatt gtaccaggat tattattagc cctcgtcggc gtctgctggg tgttaggcgg
    36721 tgacaatggg ttacattatg atgaaatcat caataatatc accaccagcc cattgagtta
    36781 tttcctggcg ttcattggtg cgtttatctg ggcagcctac tgcacagtaa cgaataaata
    36841 cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta acgggagcaa gtctgtgggt
    36901 ttactatttt ctcacgccac aaccagaaat ggtatttagc acgccagtca tgattaaact
    36961 catctctgcg gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatactgca
    37021 tggcaacgtc accataatgg cggtaggttc gtattttacg cccgtacttt cctcagcgct
    37081 tgcagccgtg ctgctcagcg ccccgctgtc gttctcgttc tggcaaggcg cgctgatggt
    37141 ctgcggcggt tccctgctct gctggctggc gacacgtcgt ggctaaaata attgccgggt
    37201 ctacggcccg gcaattaatc agataaaccc cgacaat
//
LOCUS       DABSYT020000039        35081 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.039, whole genome shotgun sequence.
ACCESSION   DABSYT020000039 DABSYT020000000
VERSION     DABSYT020000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.039"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            146..742
                     /locus_tag="ID725_RS11720"
                     /old_locus_tag="pgaptmp_002344"
     CDS             146..742
                     /locus_tag="ID725_RS11720"
                     /old_locus_tag="pgaptmp_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7850934.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            829..1554
                     /locus_tag="ID725_RS11725"
                     /old_locus_tag="pgaptmp_002345"
     CDS             829..1554
                     /locus_tag="ID725_RS11725"
                     /old_locus_tag="pgaptmp_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGV7850935.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1596..4196
                     /locus_tag="ID725_RS11730"
                     /old_locus_tag="pgaptmp_002346"
     CDS             1596..4196
                     /locus_tag="ID725_RS11730"
                     /old_locus_tag="pgaptmp_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HGV7850936.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            4210..4758
                     /locus_tag="ID725_RS11735"
                     /old_locus_tag="pgaptmp_002347"
     CDS             4210..4758
                     /locus_tag="ID725_RS11735"
                     /old_locus_tag="pgaptmp_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7850937.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4773..5378
                     /locus_tag="ID725_RS11740"
                     /old_locus_tag="pgaptmp_002348"
     CDS             4773..5378
                     /locus_tag="ID725_RS11740"
                     /old_locus_tag="pgaptmp_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7850938.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            5399..5989
                     /locus_tag="ID725_RS11745"
                     /old_locus_tag="pgaptmp_002349"
     CDS             5399..5989
                     /locus_tag="ID725_RS11745"
                     /old_locus_tag="pgaptmp_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGV7850939.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            6001..7110
                     /locus_tag="ID725_RS11750"
                     /old_locus_tag="pgaptmp_002350"
     CDS             6001..7110
                     /locus_tag="ID725_RS11750"
                     /old_locus_tag="pgaptmp_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7850940.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            7245..8039
                     /gene="panB"
                     /locus_tag="ID725_RS11755"
                     /old_locus_tag="pgaptmp_002351"
     CDS             7245..8039
                     /gene="panB"
                     /locus_tag="ID725_RS11755"
                     /old_locus_tag="pgaptmp_002351"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGV7850941.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8051..8902
                     /gene="panC"
                     /locus_tag="ID725_RS11760"
                     /old_locus_tag="pgaptmp_002352"
     CDS             8051..8902
                     /gene="panC"
                     /locus_tag="ID725_RS11760"
                     /old_locus_tag="pgaptmp_002352"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGV7850942.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9201..10097)
                     /gene="rpnC"
                     /locus_tag="ID725_RS11765"
                     /old_locus_tag="pgaptmp_002353"
     CDS             complement(9201..10097)
                     /gene="rpnC"
                     /locus_tag="ID725_RS11765"
                     /old_locus_tag="pgaptmp_002353"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGV7850943.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            10371..10751
                     /gene="panD"
                     /locus_tag="ID725_RS11770"
                     /old_locus_tag="pgaptmp_002354"
     CDS             10371..10751
                     /gene="panD"
                     /locus_tag="ID725_RS11770"
                     /old_locus_tag="pgaptmp_002354"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGV7850944.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10755..11984)
                     /gene="yadE"
                     /locus_tag="ID725_RS11775"
                     /old_locus_tag="pgaptmp_002355"
     CDS             complement(10755..11984)
                     /gene="yadE"
                     /locus_tag="ID725_RS11775"
                     /old_locus_tag="pgaptmp_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGV7850945.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12048..12488)
                     /gene="yadI"
                     /locus_tag="ID725_RS11780"
                     /old_locus_tag="pgaptmp_002356"
     CDS             complement(12048..12488)
                     /gene="yadI"
                     /locus_tag="ID725_RS11780"
                     /old_locus_tag="pgaptmp_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGV7850946.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(12593..13363)
                     /gene="yadH"
                     /locus_tag="ID725_RS11785"
                     /old_locus_tag="pgaptmp_002357"
     CDS             complement(12593..13363)
                     /gene="yadH"
                     /locus_tag="ID725_RS11785"
                     /old_locus_tag="pgaptmp_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7850947.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13360..14286)
                     /gene="yadG"
                     /locus_tag="ID725_RS11790"
                     /old_locus_tag="pgaptmp_002358"
     CDS             complement(13360..14286)
                     /gene="yadG"
                     /locus_tag="ID725_RS11790"
                     /old_locus_tag="pgaptmp_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGV7850948.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14395..15057
                     /gene="can"
                     /locus_tag="ID725_RS11795"
                     /old_locus_tag="pgaptmp_002359"
     CDS             14395..15057
                     /gene="can"
                     /locus_tag="ID725_RS11795"
                     /old_locus_tag="pgaptmp_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGV7850949.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15098..15634)
                     /gene="hpt"
                     /locus_tag="ID725_RS11800"
                     /old_locus_tag="pgaptmp_002360"
     CDS             complement(15098..15634)
                     /gene="hpt"
                     /locus_tag="ID725_RS11800"
                     /old_locus_tag="pgaptmp_002360"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGV7850950.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15840..18230
                     /gene="gcd"
                     /locus_tag="ID725_RS11805"
                     /old_locus_tag="pgaptmp_002361"
     CDS             15840..18230
                     /gene="gcd"
                     /locus_tag="ID725_RS11805"
                     /old_locus_tag="pgaptmp_002361"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGV7850951.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18432..19982)
                     /gene="cueO"
                     /locus_tag="ID725_RS11810"
                     /old_locus_tag="pgaptmp_002362"
     CDS             complement(18432..19982)
                     /gene="cueO"
                     /locus_tag="ID725_RS11810"
                     /old_locus_tag="pgaptmp_002362"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGV7850952.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20148..20495
                     /gene="yacC"
                     /locus_tag="ID725_RS11815"
                     /old_locus_tag="pgaptmp_002363"
     CDS             20148..20495
                     /gene="yacC"
                     /locus_tag="ID725_RS11815"
                     /old_locus_tag="pgaptmp_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGV7850953.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20601..21467
                     /gene="speE"
                     /locus_tag="ID725_RS11820"
                     /old_locus_tag="pgaptmp_002364"
     CDS             20601..21467
                     /gene="speE"
                     /locus_tag="ID725_RS11820"
                     /old_locus_tag="pgaptmp_002364"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGV7850954.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21483..22277
                     /gene="speD"
                     /locus_tag="ID725_RS11825"
                     /old_locus_tag="pgaptmp_002365"
     CDS             21483..22277
                     /gene="speD"
                     /locus_tag="ID725_RS11825"
                     /old_locus_tag="pgaptmp_002365"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGV7850955.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22315..22677)
                     /gene="yacL"
                     /locus_tag="ID725_RS11830"
                     /old_locus_tag="pgaptmp_002366"
     CDS             complement(22315..22677)
                     /gene="yacL"
                     /locus_tag="ID725_RS11830"
                     /old_locus_tag="pgaptmp_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGV7850956.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22853..25450)
                     /gene="acnB"
                     /locus_tag="ID725_RS11835"
                     /old_locus_tag="pgaptmp_002367"
     CDS             complement(22853..25450)
                     /gene="acnB"
                     /locus_tag="ID725_RS11835"
                     /old_locus_tag="pgaptmp_002367"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGV7850957.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25805..27658
                     /gene="yacH"
                     /locus_tag="ID725_RS11840"
                     /old_locus_tag="pgaptmp_002368"
     CDS             25805..27658
                     /gene="yacH"
                     /locus_tag="ID725_RS11840"
                     /old_locus_tag="pgaptmp_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGV7850958.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKAQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27729..29153)
                     /gene="lpdA"
                     /locus_tag="ID725_RS11845"
                     /old_locus_tag="pgaptmp_002369"
     CDS             complement(27729..29153)
                     /gene="lpdA"
                     /locus_tag="ID725_RS11845"
                     /old_locus_tag="pgaptmp_002369"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGV7850959.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29361..31253)
                     /gene="aceF"
                     /locus_tag="ID725_RS11850"
                     /old_locus_tag="pgaptmp_002370"
     CDS             complement(29361..31253)
                     /gene="aceF"
                     /locus_tag="ID725_RS11850"
                     /old_locus_tag="pgaptmp_002370"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGV7850960.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPEA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31268..33931)
                     /gene="aceE"
                     /locus_tag="ID725_RS11855"
                     /old_locus_tag="pgaptmp_002371"
     CDS             complement(31268..33931)
                     /gene="aceE"
                     /locus_tag="ID725_RS11855"
                     /old_locus_tag="pgaptmp_002371"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGV7850961.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34092..34856)
                     /gene="pdhR"
                     /locus_tag="ID725_RS11860"
                     /old_locus_tag="pgaptmp_002372"
     CDS             complement(34092..34856)
                     /gene="pdhR"
                     /locus_tag="ID725_RS11860"
                     /old_locus_tag="pgaptmp_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGV7850962.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8687 a         9258 c         8586 g         8550 t
ORIGIN      
        1 aaaaaaccta gaatagcaac aattcaatac accacgtaaa tatattcaca cccgtgataa
       61 ttcaccccta ttacatcctc caaaaacttt gagtaatatc cgctcgcaat attaatttca
      121 tataattttt gtaaggatgc atgtaatgaa aaaagcactt ctcgcagccg ctctggttat
      181 ggcttctggt tccgccctgg ctgtagatgg tggtcatatc gactttaacg gtatggtaca
      241 gtccggtacc tgtaaagtgg gtgtggtaga tactggtatg catagcgtta ccactgatgg
      301 cgtggttacc ctggatactg cgaatgttac tgatactttt gctgaagtta gcgcaactgc
      361 tgtcggttta ctgccgaaag agttcatgat ttctgttgag tgtgatccag gtgctccgaa
      421 gaatgctgag ttaactatgg gttctgcaag ttacgcgaac accagcggta ccctgaataa
      481 caatatgaac atcactgtta acggtattgc accggctcag aacgtaaaca ttgcagttca
      541 taacatgaaa aacaaagctg gcgctgctga aattaagcag gtccatatga acaactcttc
      601 tgaagttcag gaactgacat tagacgcaga aggtaaaggc cagtacgtat ttaacgcatc
      661 ttacgttaaa gcaccgaaca gcccggctgt aactgctggt catgtaacca ctaacgcgct
      721 gtacaccgtt gcttataagt aattgatggt gtgttgagca taaggtttgc cagcaggcaa
      781 accttatttt cgcgaattta atctttctac agacagcaag gagtgcttat gtttcccttg
      841 gttaaaaaaa cggttagtgc attatttgtg agtactctgt tagccagtgc accagcattc
      901 gctgacatta ttatttccgg aacacgtatc atttataacg ctgataaaaa agatgttaac
      961 gttcgtctgg aaaataaagg gaatcgcccc ttactgattc agaactggtt ggatacgggc
     1021 gatgacaatg ccgatcccag ccagatcaaa gtgccgttta ccgcgacccc accagtttca
     1081 cgcgtggagc caaaacgcgg tcaaaccgta aaagtgatgt ataccggtgc aacagccctg
     1141 ccgaaagacc gtgaaagcgt ctactggttt aacgtgctgg aagtgccgcc taagccgaaa
     1201 gatgctgagg ctgacaaaaa cttgttacag ctggctttcc gcacacgcat caaactcttt
     1261 tatcgcccga gtgggttaca gggagaaccg gctgaagcac cggcgaaaat cacctggaaa
     1321 ttaaacaacg ctcagttaca ggcaaataac ccaacgccat attacgtgtc ctttaatgag
     1381 gtcaagctgg aaagcggcgg caaaacctac aacgtcaatt ccagcatggt cacgccgttc
     1441 agccaggcaa gctttggcgt aaccagtctg ccaggaagcg tgtcatccgg aaaagtggtc
     1501 tttaaagcga tcaacgactt tggcggcaac attgatggtt ccgcaacatt ctaaaaaata
     1561 aacggattct ttttaacatc gccaggaatg caaccttgta ccagtttact catcaaaaaa
     1621 gccgtatccc gaaaaaaacg ctacttgcgg cctgttgtgc cctgttttat agcagcaacg
     1681 gtgctgcggc ggacaccgtg gaatatgaca gttccttttt aatgggaact ggcgcatcaa
     1741 cgattgatgt taaacgttat gctcaaggca acccgacacc gccgggtctc tataatgtcc
     1801 gcgtatttgt aaacggtcag gcgacttcca gcttagaaat tccgtttgtg gatattggcg
     1861 aaaacagtgc ggcggcctgt cttacccata aaaacctggc gcaacttcac attaagcaac
     1921 ctgaacagcc tgtcacttta ctcgccagag aaggtgaaga agaggattgt ctggatctgg
     1981 caaagtcata cgaaaaggcg gatgtgtgct ttgacggtag tgaccagttt ctcgatctga
     2041 cgatccctca ggcctatgtt ctgaaaagct atggcggcta cgttgaccct tctttatggg
     2101 aatcgggaat taacgctgcc acactggcat ataccctgaa cgcgtatcac acaagttcag
     2161 ataacgacaa tagtgacagc gtctatggcg cgttcaactc aggtatcaat ttaggagcct
     2221 ggcactttcg tgcgcgcggt aactataact ggacaacaga taacggcagc gatttcgatt
     2281 tccaggatcg ttacttacag cgtgacattc cggcaatccg ttcccagata attatgggtg
     2341 atgcctatac caccggtgaa acgtttgact ctgtcaacgt ccgtggtgtt cgcctgtaca
     2401 gcgacagccg tatgctgcct tcggcgctgg ccagttacgc tccgaccatc cgcggtgtag
     2461 caaactccaa cgccaaagtc accgtgacgc aaagcggata taaaatttat gaaaccaccg
     2521 ttccgcccgg tgaatttgtt atagacgaca ttagcccttc cggctttggt agcgaactgg
     2581 tcgtgaccat tgaagaagcg gatggttcca aacgcacctt tacgcaaccc ttctcgtcgg
     2641 ttgtacaaat gcaacgtcct ggtgtgggcc gttgggattt cagcgcgggt aaagtcattg
     2701 atgacagtct gcgatccgaa cccaatatgg ggcaagcctc ttattactat ggtctgaata
     2761 acctcttcac gggttatacc ggcattcagt tcaccgataa taactatctt gccgggctgt
     2821 taggtgtggg tatcaacacc agcatcggcg cctttgcggt agacgttacc cattcccgtg
     2881 ctgaaattcc ggatgataaa acctaccagg ggcaaagtta tcgcgtgacc tggaacaaac
     2941 ttttccagga taccgggaca tcatttaacc tcgcggcgta ccgctattcc acccaggatt
     3001 acctgggcct gcatgatgcg ttagtcctca ttgacgacgc caagcatttg tctgccgatg
     3061 aagacaaaaa caccatgcag acgtactcac gtatgaaaaa ccagtttacc gtcagcatta
     3121 accagccatt gaatatcgcc tatgaagatt acggttcgct gtttatttcc ggtagctgga
     3181 cgtattactg ggcggcgaac aatagccgca ctgaatataa tgttggttac agtaaaagcg
     3241 tttcgtgggg cagtttcagc gtcaacctac aacgtagctg gaatgaagac ggcgagaaag
     3301 atgacgcgat gtacgtcagc gttagcgtac ctattgagaa tattttaggt ggcaaacgta
     3361 agtcttctgg tttccgcaat ttaaatactc agctcaatac cgatttcgat ggttcacatc
     3421 agttgaatgt taacagttcc ggtaacactg aaaacaatct ggtgaactac agtgtcaacg
     3481 caggttatag cctcgataaa aacgccggcg atttagcctc tgttggtggt tatctcaact
     3541 atgaatctgg gttaggcggt atttccgctt cggcctcggc cacttctgat aacagccaac
     3601 agtactccat ctcaaccgat ggcggctttg tattacacag tggtggttta acgttcacta
     3661 acaacagttt cagcagtaac gacacgctgg tgttaatcaa cgccctaggt gctaaaggcg
     3721 cacgaatcaa taacagtaat aacgaaatcg atcgctgggg atatgccgtg acgtcctctg
     3781 tcagcccata tcgtgaaaac cgggtaggtc tgaacattga aacactggaa aacgatgttg
     3841 aactgaaaag taccagcgcc accaccgtac cacgtagcgg ctccgttgtt ttgacccgtt
     3901 tcgaaactga cgaggggcgt tctgccgtgc tgaatattac tgccgccaat ggcaaatcca
     3961 ttccgtttgc tgcggaggtt taccagggtg aggtgatgat cggcagcatg ggccagggtg
     4021 gtcaggcatt tgtacgcggt attaacgaca gcggggaatt aatcgtgcgc tggtatgaaa
     4081 acaaccaaac cattgactgt aagttgcact accagttccc ggcgcagcca caaacgcagg
     4141 gaagcaccaa caccttatta cttaacaatc ttacctgtca ggtagcaaat cactaatatg
     4201 gaaaattcta tgaaaaggat actactaaca tccgcgttaa taggcctggg tttaccggcg
     4261 gttggctctg caacagatct taatgtcgat tttacggcca ccgtattagc caccacctgc
     4321 acgataacta tagtggaaga tggtggtcca gcagtgaccg gtagtaacga tgaatacagc
     4381 ctgacaattc ctgatgttgg tctggataaa gtcgctaccg ctgcgcccga agctcaggcc
     4441 aactttaaat taaaggcctc cgattgcagc aatggttact caaagatttt taccacactt
     4501 acaggaacaa cggtttccgg taagttaatt gttaatgaag caacctctgg cgctgctggg
     4561 gtggggatgg ggattaagcg tagagatacc gcagactcga ctttcttcac acccaataat
     4621 actgacaaat ttgaatggag cgccgatgag aaagccagcg gtgtgcctct gaccgtagca
     4681 ctgcgcgaaa caacggcggg agcaggacgc accggtgcgt ttcaggcaaa agccaccttt
     4741 aacttcacct acgaataaca gggacagcca ccatgaaatt aaaagtcatc gctacactga
     4801 ttgctactgt tgccgtgggt gtaagcttta acagcaattt tgcttctgcg agtacaacgt
     4861 ccgcttcttt aaccgtaaac agtaacctga ctatgggtac ctgcagtgct cagataatgg
     4921 ataatagtaa taaagtgatc aatgaagtgg tctttggcaa tgtttatatt tctgaactcg
     4981 gtgcaaaaag caaagtgcaa cagtttaaaa ttcgctttag caattgctct ggccttcccc
     5041 aaaacagcgc ccaaatagtg ctggcaccta atggtatatc ctgtgctggt tctcaatcgt
     5101 catcggcggg tttttctaac aagtttactg acgctagcgc agcaaccaga acggctgtgg
     5161 aagtatggac tacagataca ccggaaagca atggcagtac gcaattccat tgtgctcaaa
     5221 agataccagt gcctgtgacg cttcccgccg acaccacaac tcagccttac gattacccgt
     5281 taagtgcacg gatgaccgtt gcggaaggta gattggtaac cgatgtaaga ccgggtaatt
     5341 tccgctctcc cacgactttc acgatcactt atcagtaata gcggataagg atattagaat
     5401 gctatgcagg catcataaaa tcgtacactt tttagggctg gcaacagccc ttatcacgcc
     5461 ttttgcctat tccggccagg atgtggatct caccgcaaag atcgtgccca gcacctgtca
     5521 ggttgaagtc agtaataatg gcgttgtcga tctcggcaca gtgacgctgg actattttgc
     5581 cgacaatgta accccgacaa cggattatgc gggtggcaaa acgtttaatg tgaatgtggt
     5641 ctcttgcgat aacattcaaa cgacacagtc gcaaatgaaa ttagattttc agccgcaggc
     5701 gggatcgctt gcacaggcga ataatcaaat ttttagtaat gaatatgaac agcaggctac
     5761 cggggcgaaa aacgtcggca ttgtgatttt ttcggcccaa ccgaatcagc agacatttaa
     5821 cgtgcgagga acggatggtt cctcaacagc aatttattcg gtagcacctg gcaatgctgt
     5881 tccctcaacc tggacctttt attcccgtat gcagcgggtg aataatgccc ttcctcctga
     5941 atcgggaatg gtgagaagcc aggttattgt taacgtatct tacgaataat aaggataatt
     6001 atgcaaagga aaggcaataa actgttgatt cagttatgca gtgtgatact gctatttttt
     6061 accacatcct ggtatgcatt ggcgaatgaa tgttatatag agagaaatgc tgaaggggat
     6121 tatcacatga agataagctc tactcagctt agtctggcgt cacaaatggt cgaggttccg
     6181 acagaaatag ccgaagctac atgggatgta aatattcaac taagaggcga tgccataggg
     6241 tgtaaatctc ttggggatag taaggcagtt cactttctta atacagctga cccaagttta
     6301 atatccacgt acaccacaac gaatggcgca gcgttattaa aaacaactgt tccaggcatt
     6361 gtgtattctg tcgagttatt atgccttagt tgtggtgccg cagatgaact tgatttatgg
     6421 ctacctgcac aaagtggcgc agataacttc ataccaagca cccagacgaa atgggcctat
     6481 gagtacagtg atcaaagttg gtatttacgt tttcgcttat tcataactcc tgaatttaaa
     6541 cccaagaatg gtgtttccag cggaacaacg atagcaggaa agattgcgtc atggtatata
     6601 ggtaccaatg accagccgtg gatcaacttt tacattgaca atgactcttt aaagtttttc
     6661 gtcgatgaac cgacctgtgc aacagttgcc ctggcacaag atcagggcaa cgtcagtggc
     6721 aatcaggtaa cgcttgggaa cagctatgtt tcggaagtga aaaatgggct tacgcgggaa
     6781 atcccttttt ctatccgtgc tgaatactgt tatgccagta aaattacggt taagttgaaa
     6841 gcggcaaata aacccagcga tgccacactg gtgggtaaaa cgactggctc ggcttcaggc
     6901 gtggctgtaa aagtaaattc aacttatgac aatagcaaag tattgttaaa agcagatggt
     6961 agcaacacgg ttgactacaa cttcgccgcc tggtcaaaca acctgctgtt tttacctttt
     7021 acggcgcagc tggtaccgga tggtagcggt aatgctgtcg gtgttggaac attttcaggt
     7081 aacgcgacct tctcctttac ctacgaataa gtaaaacgtc attccctcct catcgcacca
     7141 gagttctgct ctggtgcgaa catttcccca cactaataac cattgcctcc ctgttttcgg
     7201 ctgttagaat gcgatcaatc tgactttagc catcaggata cgttatgaaa ccgaccacca
     7261 ttgcttcatt gcagaaatgt aagcaggaaa aaaaacgctt cgccaccatc accgcctacg
     7321 actacagctt cgcaaaactg tttgccgagg aagggctgaa cgtgatgctg gtgggcgact
     7381 cgctggggat gaccgttcag gggcatgaat ctacccttcc cgttaccgtt gaagatatcg
     7441 cctaccacac taccgcagta cgtcgtggcg cgccaaactg cctgctgttg gctgacctgc
     7501 cgtttatggc gtatgccacg ccggaacaag cctttgaaaa cgccgcaacg gttatgcgtg
     7561 ccggtgctaa catggtcaaa attgaaggcg gtgagtggct ggtcgaaacc gtaaaaatgc
     7621 tgaccgaacg tgccgttcct gtatgtggtc acttaggttt aaccccacag tcagtgaata
     7681 ttttcggtgg ctacaaagtt caggggcgcg gcgatgaagc gagcgatcga ctgcttagcg
     7741 atgcattagc cttagaagcc gctggtgcac agttgctggt gctggaatgc gtgccggttg
     7801 aactggcaaa acgtattacc gaagcgctgg cgatcccggt tatcggtatt ggcgcaggca
     7861 acgttaccga tgggcagatc ctcgtgatgc acgatgcgtt cggcattacc ggcggtcata
     7921 tccctaaatt tgccaaaaat ttcctcgctg aaacgggcga catccgcgct gcggtgcggc
     7981 agtatatggc tgaagtggag tccggcgttt atccgggcga agaacacagt ttccattaag
     8041 gagtgacgtt gtgttaatta tcgaaaccct gccactgctg cgccagcaaa ttcgccgcct
     8101 gcgtatggaa ggcaagcgcg tggcgctggt acctaccatg ggcaacctgc atgatggcca
     8161 catgaagctg gtcgacgaag ccaaagcccg cgccgatgtg gtcgtcgtca gtattttcgt
     8221 taacccgatg cagttcgacc gcccggagga cctggctcgg tacccacgca ccttgcagga
     8281 agactgcgaa aagctgaaca agcgcaaagt ggatctggtt ttcgcgccct cggtaaaaga
     8341 gatctacccg aacggtactg aaacccatac ttacgttgac gttccagggc tttcgaccat
     8401 gctggaaggc gccagccggc cggggcattt tcgcggcgtg tcgactatcg tcagcaagct
     8461 gtttaacctg gtccagccgg acatcgcctg cttcggtgaa aaggactttc agcaactggc
     8521 gctgatccgc aaaatggttg ctgatatggg ctttgatatt gagattgtcg gtgtgccaat
     8581 tatgcgcgcc aaagacggtc tggcactgag ttcccgtaac ggttatctga cggcagaaca
     8641 acgcaaaatt gcgcccggtc tgtacaaagt tttaagttcg attgccgaca aattgcaggc
     8701 tggcgaacgg gatctcgatg aaattattgc tattgccggg caagaactga atgaaaaagg
     8761 cttccgcgcc gatgatattc agattcgcga tgccgacaca ttgctggaag tctctgaaac
     8821 cagcaaacgg gcagtaattc tggtagccgc ctggcttggc gatgctcgcc tgatcgacaa
     8881 caaaatggtc gagctggcgt aatacttata ctctaaataa ttcaagttgc aagttgcagg
     8941 aaggcggcaa gcgagtgaca aattcgtcag gaacgaattt gcccagccgc aggctggcct
     9001 tcggtgagag acatggatgt ctctcattaa tccccaggag cttacataag taagtgactg
     9061 gggttagcga acgcagacgc agcacatgca atttgaagta tgacgagtat aactggcgct
     9121 acggctgatg gcgccaatta ttaatttgcc ccacgtcatg gcgatggcgt ctgtaagcaa
     9181 ttattcataa cgcagacaac ttagttaatc agcctatcaa tctcagcaag agataaattt
     9241 gtcatctctg caacgtcttc ccgagacatc cctttactca gaaaacgctg ggcgaattct
     9301 tgcctctcct cctgtcttcc ctgctgaatc cccttctcaa tccctttctc ttcaaaccac
     9361 tgtgccagcg tcatcataga cttccctccc gtttccctgt ctctcagcac accataaaac
     9421 aaatccgctt gttcagtatg accgcgttgc agcatatagt tttgcatggc aactaactga
     9481 ctttcgctag tgtacccttc gtcaatcagc gtgaccagtt gctccagcaa taacattaag
     9541 tcgcgctggc gaatatgttt ttgcagtagt tcgagaatcg caatccgccg atgttgcatg
     9601 atttcgtcat ccggtgtgat ggtgatatcc accagcggga aaggactgtt atagacgcgt
     9661 cgcgccagct ccggcgagta aaacatatca aaccagcaca ttgagagcgg ataaggtgtg
     9721 gcctcgccct gataaaagag aatcggcact accagcggta gtttgtcgtg atccgcctcc
     9781 agatgccgat gcatggcggc tatagaataa cgcatcatgc gaaaggccat tttcttatcc
     9841 ggcttgcttt ggtgttcaat cacaacatgc agataaccgg gattgccctg catttgcacg
     9901 gaatagagca cgtccgtgct gtgtccttta aggctctctt caatgaaact ccccgactct
     9961 aaatgaagcg tgttgaggtc acaaagttcg cgtaattcca ctggcaaatg tatctccaga
    10021 aagtcgcgag ccgtctccgc atgcattaaa aattgtttaa ataccgcgtc atgcggtgtg
    10081 gtactcggtg catccatctt ccctgtcttc cgtgttgtct tgcgtgaagg cagtatccag
    10141 tcaaggcgtg ctatcgtcag ccacctcttt attgtttacc gagcagcgtt cagagaaata
    10201 tcaattcgtt acaggcgata catggcacgc ttcggcgcgg tacgcaaact gctgatgcaa
    10261 atgccgtcag atgagtagac actaaacaaa aatcgggcaa tactgcgtga gaatttcctc
    10321 aaagcaggca ccgcctgctt cgttaacgac agggtagaaa ggtagaagtt atgattcgca
    10381 cgatgctgca gggcaaactc caccgcgtga aagtgactca tgcggacctg cactatgaag
    10441 gttcttgcgc cattgaccag gattttcttg acgcagccgg tattctcgaa aacgaagcca
    10501 ttgatatctg gaatgtcacc aacggcaagc gtttctccac ttatgccatc gcggcagaac
    10561 gcggttcgag aattatttct gttaacggtg cggcggccca ctgcgccagt gtcggcgata
    10621 ttgtcatcat cgccagcttc gttaccatgc cagatgaaga agctcgcacc tggcgaccca
    10681 acgtcgccta ttttgaaggc gacaatgaaa tgaaacgtac cgcgaaagcg attccggtac
    10741 aggttgcttg attgttatcc ctgcggctgg ttactcacca gccgcgacat cgtctccagc
    10801 gaatccgttc ttaagatata aagtcgtttt agtaacaacg gattatcccc cggttttact
    10861 ttgcctttca tggttgtcac cgccaggtga aatccggcat cgtttgctgc cttcacggct
    10921 ttgtcattaa agccgccaaa cggatacgaa agataccaga catgcggatt aaattgcgcc
    10981 agagcgcgac gtgaacgtgc aaaatcaaac agaatattgt gctcactacg gctcagtaat
    11041 atgggtcggc gataaccatc tacccgatgc aaaaaatggg tatgtgactg gaaatcaaat
    11101 acatcgcgaa tttcgttaag ctcagaaacg ctcataaatt gcagcgattt tggttcccac
    11161 ttctgcgggt gacgtttgat gcgcgaggta acaataaacg ccgtcgcctt catgccatat
    11221 tgtttcaaca caggatacgc atagcggctc actgacttga ggccatcatc aaaggtaatc
    11281 accaccgctc gcgcaggaag attgatctta ttcttcacgt aaccttccag ctgcaccatg
    11341 ctcagtgtcg cgtatcccct gtcgcgcagc caggccatct ggttattgaa agcgcgtacc
    11401 gaggtggtcg tcgaagtatg gcgaaaacgg gtgttttctt cgtcgcgcag aatatggtga
    11461 taggttagca ccggaaggcc attatcgggt tgggcatcca gcgcgctgat ataggccagt
    11521 cgatcgccaa tacggatctg ataccaggtt tgatttaacc tgtctttcag tttatgcaaa
    11581 atcgggtagc gcaaattgtc cgccagtacc ccaaatggcg cactgcccac actcggcgcg
    11641 ttatagaccg gcgtatcttt ccaggtaact aagttctgat tactcagcgg cttgttgagg
    11701 tcacccaaac cgtcttcaac tttttgtcgc ccctgaaccg gctcaagatg acctttatcg
    11761 ataaaccctt tatcaaagcc aaaattaaat gcgtaataac ttgcggcagt gggctccacg
    11821 gcaatgattt gtccggcccg aatattcccc acggtcacca tcttgtcacc aatttgcgcc
    11881 cagaccgcag cattttcgat ggtttgcata taacgggcag gtaacgcggc actgacactt
    11941 gcggtaaaca gcatcagcag gagaataaca gcttgtttgt acatgaaaaa atcaccgagg
    12001 gtgaaacaac gcttttattg tagcaaaagc gccatcagaa tcgcgggtta atactcatac
    12061 aaattatgtt tgaggacgaa aggcggattt ttttgttgct ggtgccaaat actactcacc
    12121 tccggcgcac ccagttcgac aatacgctcg cgaaactctg aactggtcat ggtttcacgg
    12181 caagccatca tctgttcaat taacggtaac gtgacaccag aaatcacttc gcaacgggag
    12241 tgtttgtggc ttaataatga agccacgcgg tacggtggcg ctccggctat atcggttaag
    12301 aagatgacac cctcaccaga gtccgcttca tgcagagcat cgcacatcat gcggctgagc
    12361 atattagaac ttaatccgcg ccagaaattc acggcccggc actgaagaag cgccccatgt
    12421 tttttctcca gcacatccag tatctcttgc gcccggtcat cgtgacaggt aattacccaa
    12481 cctaacatcg ccttcctcct tagccgaaaa tcagtctacc ctgaccattg ttgataaagg
    12541 gtgatagccg tcaaaactcc tctcccccaa atccagagga gaggaaatag ccttagctac
    12601 gcaaaccacg tccacgttgg atcagcgacc aacagatcaa ataaaacgcc acaataaaga
    12661 ccaccagtac gccaaaggta gtgaccagcg gaacatcatt gataccgagg aagccgtagc
    12721 ggaaaccact gatcatataa acgattgggt tcaggtgcga cagcccttgc cagaacggcg
    12781 gcaacaaagt cagtgagtaa aagaccccac ccaaatacgt gagtggcgtt aacacaaagg
    12841 ttggcaccag gctgatgtca tcgaacgttt tggcaaacac accgttcagc aaacccgcaa
    12901 gggagaacaa caccgccgtg agcaccaggg ttaaggcaac gaatacccac gaatgcacct
    12961 gaaacggcac aaaaaacagt gaaattgccg tcaccagaat gccaacaaac agaccacgcg
    13021 ccacgccacc gccgacatat ccggcaataa tgacgtgagt cggaaccggc gctaccagca
    13081 gctcttcaat attacgctgg aacttggcac caaaaaatga tgacgcgacg ttggcgtagg
    13141 cgttggtgat caccgacatc atgatcagcc ccggtacgat gaactgcatg tagctgaagc
    13201 catgcatatc gccaatacgc gaaccaatca ggttaccgaa aataataaag taaagggtca
    13261 tggtgatgac tggcggcacc agcgtctgca cccagatacg cataaagcga tggatctctt
    13321 tcgcccagat gctttttagc gccacccagt aaagatgcat catgcgcgat ctccttgttt
    13381 ttcattaacc agtgaaacaa acagctcttc cagacggtta gctttgttac gcatacttaa
    13441 tacctgaatg ccctgctcac ttaactgcgt aaatacgctg ttgatcccct gctcacgcag
    13501 cacttcaact tccagcgtcg cggtatcgac cagtcgatac tgatagccat cgagcttcgg
    13561 taacggactt ttcggtgcga gatcgagaat aaaggtttcc gatttcagct tcgccagcag
    13621 cgccttcatc gaggtatttt ccaccagctc accgtgttga ataatgccga tattgcggca
    13681 cagcatttct gcttcttcca ggtagtgtgt ggtgagaatg atggtggtgc ctttgtcgtt
    13741 taaatccttc aaaaagcccc acattgagcg gcgaagttca atatccacgc ctgcagtcgg
    13801 ctcgtcgaga atcagtagtt taggttcatg cattaacgca cgggcaatca ttaaacggcg
    13861 cttcatcccg ccagataaca tgcgcgcacg ttcgttgcgt tttccccata aatcgagctg
    13921 cttaagatat ttttcgctgc ggatgtacgc ctctttgcgc tccacaccgt agtaccctgc
    13981 ctgattcacc acaatttgct gcacggtttc aaacgggttg aagttaaatt cctgcggcac
    14041 cagtcccaac tgacgtttag cgttcacgac atctttctcg agatcgtaac caaatacgct
    14101 gacccgcccg gaggttttat ttaccagaga gctgataata ccgatagtgg tcgatttccc
    14161 ggccccgttc ggcccgagaa gcgcataaaa atcacccgct tcgacctgca aatctattcc
    14221 acgaagcgcc tgaacgccgc ctggatagtt ttttttaagc tgttgaagtt ccagtgcaat
    14281 ggtcataaat ttttacttac cttacgttct tacactttat ctgtggttta aatcgtcccg
    14341 gagttgccct atattagcca aacgtaatta tttggttaca ggtcgttaac ctccatgaaa
    14401 gacatagata cactcatcag caacaatgca ctatggtcaa aaatgctggt ggaagaggat
    14461 cccgggtttt ttgagaaact ggcacaagcg caaaaaccgc gctttctatg gattggatgt
    14521 tccgacagtc gcgttcctgc agaacgttta accggtcttg agccgggcga actctttgtt
    14581 caccgtaatg ttgctaacct ggtcattcac accgacctga actgcctttc cgtggttcag
    14641 tatgcagtgg atgtactcga agttgaacac attattatct gtggccacta cggttgcggc
    14701 ggcgtacaag ccgcagttga aaacccggaa ctggggctta tcaacaactg gctgctgcat
    14761 atccgcgata tctggttcaa acatagctca ttgctcgacg aaatgccgca agagcgccgt
    14821 ctggatacct tgtgtgaact gaacgtcatg gaacaggtgt ataacctggg ccactccacc
    14881 attatgcaat cagcgtggaa acgcgggcag aaagttacca ttcacggctg ggcttatggc
    14941 attcacgacg gcttgctgcg tgatctggat gttactgcca ccaaccgcga aacccttgag
    15001 caacgttacc gtcacgggat ttccaacctc aagctgaaac acgccaacca caaataaaaa
    15061 tgccatgccg gatgcaacac atccggcaac ttcacactta ctcgtccagc agaatcactt
    15121 tgccgatata cggcagatga cggtaacgct gtgcgtaatc aatgccgtaa cccaccacaa
    15181 actcatccgg gatcgagaaa ccgataaatt ctaccgggac gttcacttca cgacgggacg
    15241 gtttatccag cagcgtacaa atcgccaacg acttcggttc gcgcaggctt aagatctcac
    15301 gcactttcga cagtgtattc cccgagtcga tgatatcttc aacaatcagc acatccttgc
    15361 cacggatatc ttcatccaga tctttgagga ttttcacatc acgggtggtg gacatgccgc
    15421 taccgtagct ggaggcggtc ataaagtcga cttcatgaga tacctgaact tcacggcaca
    15481 ggtccgccat aaacataaat gagccacgca gcagacccac cagcaccata tcgctgccgc
    15541 tgtctttgta acgctcagta atctgacgac ccagttcggc gatacgcgct ttaatctccg
    15601 cttcggggat cattacttct acagtatgtt tcatatctct aaccatatga tttaaaaaca
    15661 aatcacttcg cacgggcagg ttttggcacc gcaacaaagc gcaagacacg cagtatacca
    15721 gcaaaacgat tcattcgcgg aatctgttaa taaagctcaa attgtgatga cgatcacaca
    15781 tgttaaaacc tataattaat tgctactaaa atattaatga attgaaatgg tgtctcttta
    15841 tggcaattaa caatacaggc tcgcgacgat tactcgtcac gctaacagcc ctttttgcag
    15901 cgctttgcgg gctgtatcta ctcattggcg gaggctggct ggtcgcgatt ggcggctcct
    15961 ggtactaccc tatcgctggc cttgtgatgc tcggcgtcgc ctggatgctg tggcgcagta
    16021 aacgtgccgc gctttggcta tacgccgccc tgctgctcgg caccatgatt tggggcgtct
    16081 gggaagttgg tttcgacttc tgggcgctga ctccgcgcag cgatattctg gtcttcttcg
    16141 gcatctggct gatcctgccg tttgtctggc gtcgcctggt cattcctgcc agcggcgcag
    16201 ttgccgcact ggtggtggca ctgctgatta gcggtagtat cctgacctgg gccggattta
    16261 acgatccgca ggaaatcaac ggcaccttaa gcgccgatgc cacacctgcg gaagctatct
    16321 cacccgttgc cgatcaggac tggcctgcct atggtcgcaa tcaggaaggt caacgctttt
    16381 cacctctgaa acaaattaac gccgataacg tccataatct gaaagaagcc tgggtgttcc
    16441 gtactggcga tgtgaagcag ccgaacgatc cgggcgaaat caccaatgaa gtgacgccga
    16501 ttaaagtggg cgacaccctt tacctgtgta ccgctcacca gcgcctgttt gcgctcgatg
    16561 ccgccagcgg caaagagaaa tggcattacg atcctgagct gaaaactaac gagtctttcc
    16621 agcacgtaac ctgccgtggc gtctcttatc atgaagccaa agcagaaacg gcttcgccgg
    16681 aagtgatggc ggattgcccg cgtcgtatca ttcttccggt caatgatggt cgcctgattg
    16741 cgattaacgc tgaaaacggc aagctgtgcg aaaccttcgc caataaaggc gtgctcaatc
    16801 tgcaaagcaa tatgccagac accaaaccgg gtctgtatga accgacttcg ccaccgatta
    16861 tcaccgataa aaccatcgtg atggcaggtt cagttaccga taacttctca acccgcgaaa
    16921 cgtctggcgt gatccgtggt tttgatgtca acaccgggga gctgctgtgg gcttttgatc
    16981 cgggcgcgaa agatccgaac gcaatcccgt ctgacgaaca cacctttacc tttaactcgc
    17041 caaactcgtg ggcaccagcg gcctatgacg cgaagctgga tctggtgtat ctgccgatgg
    17101 gcgtgaccac gccggatatc tggggcggta accgcacacc ggaacaggaa cgttatgcca
    17161 gctcgattct ggcgctgaat gccacaaccg ggaaattggc gtggagctac cagaccgttc
    17221 accacgacct gtgggatatg gatcttccgg cacagccgac gctggcggac atcaccgtta
    17281 atggtcagaa agtgccagtt atttacgctc cggcgaaaac cggcaacatt tttgtgctcg
    17341 atcgtcgtaa tggcgaactg gtggttccgg caccggaaaa accggttccc caaggtgctg
    17401 ccaaaggcga ttacgttacc cctactcaac cattctcgga actgagcttc cgtccgacga
    17461 aagatttgag cggtgcggat atgtggggag ccaccatgtt tgaccaactg gtgtgccgcg
    17521 tgatgttcca ccagatgcgc tatgaaggca ttttcacgcc gccatctgaa cagggcacgc
    17581 tggtcttccc gggtaacctg ggaatgtttg aatggggcgg gatttccgtt gatccaaatc
    17641 gtgaagtggc aattgccaac ccaatggcac tgccgtttgt ttcgaaactg atcccacgcg
    17701 gtccgggtaa cccgatggag cagccgaaag atgccaaagg cacgggtacg gaatccggca
    17761 ttcagccaca gtacggtgta ccgtatggtg tcacgctcaa cccgttcctc tcaccatttg
    17821 gtctgccatg taaacagcca gcatggggtt atatctcggc gctggatctg aaaactaatg
    17881 aagtggtgtg gaagaaacgt attggtacgc cgcaggacag tatgccgttc ccgatgcctg
    17941 taccggtgcc gttcaatatg ggtatgccga tgctgggcgg gccaatctcc acggcgggta
    18001 acgtgctgtt tatcgccgct acggcagata actacctgcg cgcttacaac atgagcaacg
    18061 gtgaaaaact gtggcagggt cgtttaccag cgggtggtca ggcgacgcca atgacctatg
    18121 aagtgaatgg taagcagtat gtggtgatct ccgcaggcgg tcacggttca tttggtacga
    18181 agatgggtga ctatattgtg gcttatgcgc tgccggatga tgtgaagtaa gacttgctca
    18241 gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc gtgaacgcct tatccagcct
    18301 acaaaattgt gcaaaatcaa tggattgcac aggtaacgta ggcctgataa gcgtagcgca
    18361 tcaggcagat ttgcgtttgt catcagttta atgcccggag agatccgggc atattcccga
    18421 atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg ctccagcaga
    18481 tggcagtgcg ccatataagc acgttctttc ggtgcgtcgt gattaaactt caccagcact
    18541 tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg agccgctggc
    18601 ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa cggatgcagc
    18661 atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc cgccgcaaac
    18721 atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg gaaatcgaac
    18781 ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat catctggctg
    18841 tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg catccccatc
    18901 atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc ttccagcgaa
    18961 ggtaacgcag gcaggctact taatgtgtct ggcaaagcac cggaagcact aatagcaatc
    19021 ggctgaatcc gcattaccgg atgaggctta tcaaacggcg caatcgccat ccccatctgg
    19081 ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac cagcacttca
    19141 aaacgctcgc ccatcagcac cggcagctcg ttcaccttca ccggttcagg tagcagacca
    19201 ccgtcgctgg caatcacata aagcgggcga ttgtcgctgg tggcgaaatt gagcgagcgg
    19261 gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc gtgttgcggg
    19321 tagattgcac cgttggtcag caacgtatcg ccaaaccagc ccacggcggc ggtcatcaca
    19381 tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac gatcaccgga
    19441 acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc atcttcaatc
    19501 accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg ttgatgtgga
    19561 tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg cttgccacct
    19621 ggcggaataa ttccctgtgg gccgccgtcg acttcacccg gtacttccag cccgtgccag
    19681 tgcaacgtcg tctcttccgt cagttggtta tagatatcaa ccgttaccgc tttgccgcgc
    19741 tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt tgcagttttc
    19801 tcgccaaagg tagactgacc tgcgccaata gttaactgaa tgcgattacg ggcatcggtc
    19861 gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc acggctccac
    19921 agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc acgacgttgc
    19981 atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc atagtccttc
    20041 cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt cgtcgcctcg
    20101 ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg aagacgtttt
    20161 tcagaacagt gttattcggc agcctgatgg ccgtttgcgc aaacagttac gcgctcagtg
    20221 agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg aagaacgatt
    20281 gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt ttcgcccagc
    20341 aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc ggtgaagaca
    20401 gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaagtgcaag gccctggctc
    20461 gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt tgtagaaata
    20521 ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt tacgggtgtt
    20581 aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc
    20641 agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc gatcaccagg
    20701 atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat ggcgtagtac
    20761 aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt ccgctactgg
    20821 cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg
    20881 aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg
    20941 tgtcgttctg ccgtcagtat ctacccaacc ataacgccgg tagctacgac gatccgcgct
    21001 ttaagctggt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag acctttgatg
    21061 tcattatctc cgactgcacc gatcctatcg gtcccggcga aagccttttc acttcggcat
    21121 tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca caaaacggcg
    21181 tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc cattacttca
    21241 gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc atgacttttg
    21301 catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt caggcgcgtt
    21361 ttctcgcctc tggcctgaaa tgccgttatt acaatccggc agtccatacg gcagcttttg
    21421 ccttacccca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga gaagataaga
    21481 aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg agtttttgta
    21541 tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt gcttatatcg
    21601 atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt tccattatcg
    21661 gggctaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc agcgtcacta
    21721 ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa caccccggcc
    21781 cactgccaga aacggtcgtt gcccatctcg ataaaagtca tatttgcgta catacctacc
    21841 cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa gtctctacct
    21901 gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt gagtccgata
    21961 tcgtaaccat tgattatcgc gtgcgcggtt ttacccgcga cattaacggt atgaagcact
    22021 ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg aaggcgctgt
    22081 atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag atgttgctta
    22141 aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta accgacagcg
    22201 agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat tacgggcgca
    22261 atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat ttatttactt
    22321 ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa cgccgcatag
    22381 cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc cagcgaattc
    22441 cagttgattg gcgcgaacca tcacctcttc gccgtccatc catagcgtat actcatgccc
    22501 tgcccgctgc caggaacgtt cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc
    22561 aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca cttcatgccc
    22621 catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt aatccatagt
    22681 gtgttcctct gctcccggca atgcaccgaa atcgcattgc gccattgatg taattatcgc
    22741 agccttaccg gagaaaaaca gcggaacggc gatggtttag aaataaaaaa atgcccggga
    22801 atctcattca ccgggcattg tgtcgtttat gcgcagcgcg tgcgctgact ttttaaaccg
    22861 cagtctggaa aatcacccca tcggctttct cggtgtactg agaaagctgg ttgaagttca
    22921 gataacggta agtatcaacg gctgttttat ctacctgcgc cacgtaggtc tggtactctt
    22981 ccggcgtcgg cagtttgcca atcagcgccg caacagcagc cagttccgca gaagccagga
    23041 agacattcgc gccagtaccc agacggttcg ggaagttacg ggtagaggtg gaaaccaccg
    23101 ttgcaccgtc cgccacacgc gcctggttac ccatacacag ggaacagcca gggatctcga
    23161 tacgcgcacc actcttaccg aagacgctgt agtagccttc ttcggtcaac tgtgcggcgt
    23221 ccatacgggt tggcggtgcc acccacaggc gggtcggcaa ctgacctttg tgcgcatcca
    23281 gcagtttacc tgcggcacgg aagtgaccga tgttagtcat gcaagaaccg ataaacactt
    23341 cgtcgatctt ctcgccctgt accgcagaca gcggacgtgc gtcgtccggg tcgttcggag
    23401 cacagaggat tggctcttta atatccgcca gatcgatgtc gatcactgcc gcgtattccg
    23461 catctgcatc ggcttccagc agctcaggat tcgccagcca tttttccatg ccctgaatac
    23521 gacgttccag ggtacgacga tcgccgtaac cttccgcgat catccacttc agcaggacga
    23581 tgttggagtt caggtattcg atgattggtt ctttgttcag cttgatggta caaccggcag
    23641 cagaacgctc ggcggacgca tcggtcagct caaaggcctg ctcaactttc agatccggca
    23701 gaccttcaat ttctaggatg cggccagaga agatgttttt cttgcctttc ttctcaacgg
    23761 tcagcagacc ttgtttgatc gcatacagcg gaatggcgtg taccagatcg cgcagggtga
    23821 tgcccggctg cattttgcct ttgaagcgca ccagaacgga ttccggcata tccagcggca
    23881 ttacgcctgt tgcggctgca aatgccacca gaccagaacc cgccgggaaa gagataccga
    23941 tcgggaaacg ggtatgggag tcaccaccgg taccgacggt atccggcagc agcatacggt
    24001 tcagccagga gtggattacg ccgtcacccg gacgcagcga cacaccgcca cggttcataa
    24061 tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac gccgcggtgt
    24121 ggcagaaaga ctgcatcacc aggtcagccg agaagcccag gcacgccagg tctttcagtt
    24181 catcacgggt catcgggcct gtggtgtcct gagagcctac agaagtcatt ttcggttcac
    24241 agtacgcgcc cggacgaatg cctttcacgc cacaggcacg gcctaccatt ttctgcgcca
    24301 gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg acggaacaca tcactgtgcg
    24361 gcagaccaag tgcttcacgc gctttggtgg tcaggccacg accgataatc agcggaatac
    24421 ggccgccagc acgcacttca tcaatcagca cgtcggtttt cagttcgaag gtcgccagca
    24481 gttcgccggt ttcgtggtta cgtacttcac ctttgtacgg gtaaacgtca attacgtcgc
    24541 ccatgttcag gttagagaca tccacttcga ttggcagcgc acccgcatct tccatcgtgt
    24601 taaagaagat gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt ttgttcggca
    24661 catgtggaat atcatcgccc ataaaccaca gaacggagtt cgtggcggat ttacgcgatg
    24721 aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa acctttctgt tgcagagctt
    24781 caatctgttt gatcgggcca acaacacctg gctggtctgg ctcaatacct tcacgggcgt
    24841 ttttcagcat cgccagcgcg tgcaatggga tatccgggcg tgaccacgca tccggtgccg
    24901 gagaaaggtc atcggtgttg gtttcgccag tgactttgaa gacggtaacg gtcagttttt
    24961 cagccagcgc cgggcgattc aggaaccatt cggcatcagc ccaggactgc ataacctgct
    25021 tcgcatattc gttgcctgct ttcgctttct cttctacgtc atagaagtta tcgaacatca
    25081 gtagtgtgtg agaaagtgct ttggcagcga tcggtgccag tttggcatca tccagcgcat
    25141 cgatcagcgg atgaatgttg taaccaccct gcatagtacc cagcagttcg atggcttttt
    25201 ccggagtcag cagtggggat ttggcttcgc ctttggcgac agcagccagg aagcctgctt
    25261 taacataggc ggcttcatcg acgccaggag gaacacggtt ggttaacaga tctaacagga
    25321 attcttcttc gcccgcgggc gggtttttca gcagctctac aagcgcggcc atttggtttg
    25381 catccagggg tttgggcgca atcccctcag cggcacgctc agctacgtgc ttacggtatt
    25441 cttctagcac gacggttctc ctcgctctca ttgtcatagt gcggcaggcg attctcttca
    25501 cgctcctgtg agacagcagt ttgtagggta aatgcccagt accgcaacgg gcagaatagc
    25561 aggattttga cgacgtgtta atctgtttac aaaaaagcaa cataaaaaag tggctgaatc
    25621 gttaaggatg gtctagagat tgtttccctc cgtaaaaact tcgttaattc gcatggtgat
    25681 aatcaccttt ctcaacgaat cccaagatta gtcaaaattt aaactaccgc ctctttatac
    25741 tcggattcac agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc ttgagatccg
    25801 aattatgaaa atgactttgc cgtttaaacc ccatgtgctg gcgctaattt gcagtgccgg
    25861 gctttgtgcc gcctctgccg ggctatatat aaaaagccgc acagtggaag cgcctgtgga
    25921 aacgcaatcg acacaactgg ctgtgtctga cgctgccgca gttacgcttc ctgcaacggt
    25981 ttccgcacct cccgtaacac ccgccgtcgt caaatccgca ttcagcactg cacaaataga
    26041 tcaatgggtc gcgcccgtcg cgctgtatcc cgacgcccta ctttcgcagg tgctgatggc
    26101 atcaacctat ccgacaaacg ttgctcaagc agtgcaatgg tcgcacgata atccacttaa
    26161 acaaggcgat gctgctattc aggcggtatc tgaccagccg tgggacgcca gcgttaaatc
    26221 actggtggcc tttccacaat tgatggcatt gatgggcgaa aacccgcaat gggtgcaaaa
    26281 cctgggcgat gcttttctgg cccagccgca ggacgtgatg gactcggtac aacgattgcg
    26341 gcaactggca caacaaaccg gctcgctgaa gtcatcaacc gaacagaaag ttattaccac
    26401 aacgaagaaa actgtaccgg taacacagac agtcacggct cccgtcatac catccaatac
    26461 cgtttcaact gccaaccctg tcattacaga gcctgcaaca accgtcattt ccattgagcc
    26521 cggcaatcct gatgtggtct atattcccaa ctacaaccca accgtggttt acgggaactg
    26581 ggccaatact gcgtatccgc cggtttatct gccaccacca gccggagaac cgtttgttga
    26641 cagctttgta cgcggattcg gctatagcat gggcgttgct accacgtacg cactattcag
    26701 cagcatcgac tgggatgacg acgatcatga ccatcatcat catgacgatg ataattatca
    26761 tcaccacgat ggcggtcatc gtgacggtaa tggctggcaa cataacggcg acaacatcaa
    26821 tatcgacgtc aacaatttca accgtatcac cggtgagcat cttactgata agaatatggc
    26881 atggcggcac aatccaaact accgtaatgg tgtgccctat catgatcagg atatggcaaa
    26941 gcggtttcat caaactgatg tcaacggcgg aatgagtgcc acgcagctac ctgctccaac
    27001 acgcgacagc cagcgtcagg cggcagcaag tcagtttcag caacgaacac acgccgcccc
    27061 cgtcattaca cgagataccc aacgtcaggc agcggcacag cggtttaatg aagctgaaca
    27121 ctatgggagc tatgacgact tccgcgactt cagccgtcgc caaccactga cccagcaaca
    27181 aaaggacgcc gctcgtcagc gttatcagtc agcttctcct gagcagcgcc aggcagttca
    27241 cgagaaaatg cagactaacc cgcagaacca gcagcgaaga gaggcagcgc gtgagcgcat
    27301 tcagcccgcc tcgcctgagc agcgccaggc agtccgcgag aaaatgcaga ctaacccaca
    27361 gatccagcag cgaagagacg cagcgcgtga gcgtattcag tcagcctcgc ctgagcagcg
    27421 ccaggtgttt aaggaaaaag tacagcagcg cccactgaac caacagcaac gtgataacgc
    27481 ccgccagcgt gttcaatcag catcacctga acaacgtcag gtttttcggg agaaagctca
    27541 ggagagccgc ccacaacgtc taaacgacag taaccatact gccaggctga ataacgagca
    27601 acggtcagca gtacgcgaac gtctctctga gcgcggagca aggcgactgg aaaggtaaat
    27661 tacaggcgta aaaaaagcgg cgtggttagc cgctttttta attgccggat gttccggcaa
    27721 acgaaaaatt acttcttctt cgctttcggg ttcggcaggt cagtaatgct accttcgaac
    27781 acttctgccg ccaggcccac agactcgtgc agagtcgggt gcgcgtggat ggtcagtgcg
    27841 atgtcttcag catcacaacc catttcaatt gccaggccga tttcacccag cagctcgccg
    27901 ccgttagtac cgacaatcgc accaccgatc acacggtgag attctttgtc gaaaatcagc
    27961 ttggtcatac cgtctgcgca gtcggaagcg atagcacgac cagaagcagc ccacgggaag
    28021 gtggcggttt catagctgat gcctttctct ttcgcttctt tctcagtcag acctacccat
    28081 gcaacttctg gttcggtata ggcgatggac gggataactt tcggatcgaa gtagtgtttc
    28141 ttaccggcga taacttcagc ggcaacgtga ccttcgtgaa cacctttgtg tgccagcatc
    28201 ggctgaccga cgatatcgcc gatagcaaag atgtgcggta cgttggtacg cagctgtttg
    28261 tcaacgcgga tgaaaccacg gtcgtcaact tccacgccag ctttgcctgc gtcgaggttt
    28321 ttaccgttcg gcacacgacc aatcgctacc agcacggcgt cgtaacgctg cggttcagct
    28381 ggtgcttttt tgccttccat cgtcacataa ataccgtctt ctttcgcttc aacggcggta
    28441 actttggttt ccagcatcag gttgaatttc ttgctgatac gcttggtgaa gactttaacg
    28501 atgtctttgt cagctgccgg gataacctgg tcgaacattt caaccacgtc aatctgtgaa
    28561 cccagcgcgt ggtaaacggt gcccatttcc agaccgatga taccgccacc cattaccagc
    28621 aggcgttctg gtacttcttt cagttccagc gcgtcagtag agtcccagat acgcggatct
    28681 tcatgcggaa taaacggcag ttgaatcggg cgagaacccg ctgcaatgat cgcgttgtcg
    28741 aagttgatca cggttttacc gttctcacct tcaacttcca gggtgttagc cccggtaaat
    28801 ttacccagac cgttgaccac tttgactttg cggcctttcg ccataccagc cagaccaccg
    28861 gtcagctgat tgatcacttt ctctttccag gtacgaatct tgtcgatatc ggttttcggt
    28921 tcgccgaaga cgataccgtg ttcagccagc gctttggctt cttcgataac ttttgctacg
    28981 tgcagcagtg ctttagaagg gatacagccg acgttcaggc aaacaccgcc aagggtgttg
    29041 taacgttcta cgattacggt ttccagacct aaatcagcgc aacggaaggc agcggagtaa
    29101 cctgcggggc ctgccccaag taccacgacc tgagttttga tttcagtact catcatgacc
    29161 tctatatatt tatctccggc gggtctgacg ttttttttat gacgcccatt atccaccggg
    29221 tcgttctatc cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac aaacggcaac
    29281 cgatttgtct attcgctaat aacctcaata cattcatgag attaccagaa aaaagccggc
    29341 cgttgggccg gctcttttac ttacatcacc agacggcgaa tgtcagacag cgtgttgtta
    29401 atgatggtaa tgaaacgggc accatcagca ccgtcgatca cgcggtggtc gaaggagaga
    29461 gaaatcggca gcatcagacg cggcacgaac tctttaccat tccacaccgg ctccatcgcg
    29521 gacttggaaa cgccgaggat agccacttcc ggcgcgttca caatcggcgc gaagtgggta
    29581 gtacccaggc cgccgatgct ggagatggtg aagcaaccgc cctgcatttc gcccgcagtc
    29641 agcttaccgt cacgcgcttt cttagaaata gtcatcagct cgcgagacag ttcgatgatg
    29701 cctttcttgt tgacgtcttt gaataccgga acaaccagac cgttcggggt atccaccgcc
    29761 acaccgatgt tgatgtattt cttcagggtc agacgctgac cgtcttccga cagcgaactg
    29821 ttgaagcgag gcatctgctc aagcgctgca gcaacggctt tcatgatgaa gacaaccggg
    29881 gtgatcttca catccagctt acgtttcgcc gcttcttcgt tctgctgttt acggaacgct
    29941 tccagctcgg tgatatcggt tttgtcgaag tgagtaacat gcgggatcat cacccagtta
    30001 cggctcaggt tcgcaccaga aattttctgg atacggccca gttccacttc ttcgatttca
    30061 ccaaacttgc tgaagtccac cttcggccac ggcagcatgc cagggatacc accgccagtc
    30121 gctgccggag ctgcttctgc acgtttgata gcttctttca cgtaagcctg aacgtcttcg
    30181 cgcaggatac gacctttacg gccagtgccc ttcactttcg caaggttaac gccaaactcg
    30241 cgtgccagac ggcggatcag cggggtcgcg tgaacgtaag cgtcattttc agcaaattca
    30301 gatttgcctt ccgctttcgc agccggtgct gctgccgggg cttcagcttt tgctgccgga
    30361 gccggcgctg ctgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    30421 aagatcataa tcagcgagcc agttttcact ttatcgccaa cgttgacttt cagttccttc
    30481 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    30541 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    30601 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagccgctgg tgcaggggcc
    30661 gctgccggag ccgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgccact
    30721 tcgaagacca taatcagcga gccggtagac actttgtcgc cggtgttcac tttgatttct
    30781 ttcacggtgc ctgcaaacgg agccggaact tccatagaag ctttatcgcc ttctacggtg
    30841 atcagcgact gctcagcttc aactttgtcg ccaactttca ccaggatttc ggtcacttca
    30901 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgcggcagc ctcggggact
    30961 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    31021 tcggcggaat cgaaaatcat aatcagtgcg ccggtctgag ttttatcgcc aacagagact
    31081 ttgatctctt taacgatacc cgcctgcgga gacggaactt ccatagaagc tttgtcgcct
    31141 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    31201 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    31261 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    31321 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    31381 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    31441 gaccgaagcc atcagtaccc agtacgcggt agtcgtcagc cggtacgtaa gtacggacct
    31501 gctcagcgaa cagtttcata tagtcggtag atgccacagc cggagcgtcg ttcatcacct
    31561 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    31621 cctgaccatc acgcgccagt tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    31681 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggata gaaccggagc
    31741 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    31801 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtgg
    31861 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    31921 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc ggaatagtca
    31981 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    32041 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtcgccagcc gcccagcaca
    32101 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaacgga atcatcggca
    32161 gattgttggt gctgtaagag gtcgccgctg ccagccagga acaacctgcg cccagctcgt
    32221 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    32281 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    32341 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    32401 acttgttctt cagcatcacg ttcagggcac gaacgaaagc gatagtggta gagatctctt
    32461 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct tggcagctca agcttctcgg
    32521 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctgt gcgtgcagat
    32581 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    32641 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac accgtccatg ttcattttct
    32701 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    32761 tatgagcaag gattaccgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    32821 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    32881 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    32941 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    33001 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    33061 tcacgttcca gccagcacct tcgaagatac cttccagttc gttgatgatc ttgccgttac
    33121 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaagacc aggttatcca
    33181 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    33241 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    33301 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    33361 ggaactgcca gaattccggc atcaatttcg ggtgcggata ggaagagagg ccattgccgt
    33421 gaacttcctg acggaagtta tccagctgct cttgagtcag acgaccttcc aggaaagcac
    33481 gagcgtacac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    33541 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    33601 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcaga accgtcatga
    33661 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    33721 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gataccggtg cctgcggcta
    33781 cgttgacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    33841 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    33901 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    33961 gagttttctg gaacctgtcc cattgaactc tcgccggaaa gctcaataag acaggttcta
    34021 cgtttagttg ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    34081 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctt
    34141 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    34201 tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta
    34261 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    34321 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    34381 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    34441 tgctgcgcca gttctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    34501 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    34561 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    34621 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    34681 tcgagacgtt gaatcgcctc acgcaatgag ggacgggaga cgtcaaactg ttttgccagt
    34741 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    34801 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    34861 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    34921 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    34981 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    35041 ctttacgtag gaaagggatt ttttaacgat tattaaacta t
//
LOCUS       DABSYT020000040        34884 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.040, whole genome shotgun sequence.
ACCESSION   DABSYT020000040 DABSYT020000000
VERSION     DABSYT020000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.040"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(34..686)
                     /locus_tag="ID725_RS11865"
                     /old_locus_tag="pgaptmp_002373"
                     /pseudo
     CDS             complement(34..686)
                     /locus_tag="ID725_RS11865"
                     /old_locus_tag="pgaptmp_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(949..1239)
                     /gene="ilvN"
                     /locus_tag="ID725_RS11870"
                     /old_locus_tag="pgaptmp_002374"
     CDS             complement(949..1239)
                     /gene="ilvN"
                     /locus_tag="ID725_RS11870"
                     /old_locus_tag="pgaptmp_002374"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGV7850963.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(1243..2931)
                     /gene="ilvB"
                     /locus_tag="ID725_RS11875"
                     /old_locus_tag="pgaptmp_002375"
     CDS             complement(1243..2931)
                     /gene="ilvB"
                     /locus_tag="ID725_RS11875"
                     /old_locus_tag="pgaptmp_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGV7850964.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(3037..3135)
                     /gene="ivbL"
                     /locus_tag="ID725_RS11880"
                     /old_locus_tag="pgaptmp_002376"
     CDS             complement(3037..3135)
                     /gene="ivbL"
                     /locus_tag="ID725_RS11880"
                     /old_locus_tag="pgaptmp_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGV7850965.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            3700..3789
                     /gene="tisB"
                     /locus_tag="ID725_RS11885"
                     /old_locus_tag="pgaptmp_002377"
     CDS             3700..3789
                     /gene="tisB"
                     /locus_tag="ID725_RS11885"
                     /old_locus_tag="pgaptmp_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGV7850966.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(3913..3987)
                     /gene="ysdE"
                     /locus_tag="ID725_RS11890"
                     /old_locus_tag="pgaptmp_002378"
     CDS             complement(3913..3987)
                     /gene="ysdE"
                     /locus_tag="ID725_RS11890"
                     /old_locus_tag="pgaptmp_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGV7850967.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            4069..5253
                     /gene="emrD"
                     /locus_tag="ID725_RS11895"
                     /old_locus_tag="pgaptmp_002379"
     CDS             4069..5253
                     /gene="emrD"
                     /locus_tag="ID725_RS11895"
                     /old_locus_tag="pgaptmp_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGV7850968.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(5261..5758)
                     /gene="yidF"
                     /locus_tag="ID725_RS11900"
                     /old_locus_tag="pgaptmp_002380"
     CDS             complement(5261..5758)
                     /gene="yidF"
                     /locus_tag="ID725_RS11900"
                     /old_locus_tag="pgaptmp_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGV7850969.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(5755..6117)
                     /gene="yidG"
                     /locus_tag="ID725_RS11905"
                     /old_locus_tag="pgaptmp_002381"
     CDS             complement(5755..6117)
                     /gene="yidG"
                     /locus_tag="ID725_RS11905"
                     /old_locus_tag="pgaptmp_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGV7850970.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(6107..6454)
                     /gene="yidH"
                     /locus_tag="ID725_RS11910"
                     /old_locus_tag="pgaptmp_002382"
     CDS             complement(6107..6454)
                     /gene="yidH"
                     /locus_tag="ID725_RS11910"
                     /old_locus_tag="pgaptmp_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGV7850971.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            6563..7012
                     /gene="yidI"
                     /locus_tag="ID725_RS11915"
                     /old_locus_tag="pgaptmp_002383"
     CDS             6563..7012
                     /gene="yidI"
                     /locus_tag="ID725_RS11915"
                     /old_locus_tag="pgaptmp_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGV7850972.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(7059..8552)
                     /gene="yidJ"
                     /locus_tag="ID725_RS11920"
                     /old_locus_tag="pgaptmp_002384"
     CDS             complement(7059..8552)
                     /gene="yidJ"
                     /locus_tag="ID725_RS11920"
                     /old_locus_tag="pgaptmp_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGV7850973.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(8549..10264)
                     /gene="yidK"
                     /locus_tag="ID725_RS11925"
                     /old_locus_tag="pgaptmp_002385"
     CDS             complement(8549..10264)
                     /gene="yidK"
                     /locus_tag="ID725_RS11925"
                     /old_locus_tag="pgaptmp_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGV7850974.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            10431..11324
                     /gene="yidL"
                     /locus_tag="ID725_RS11930"
                     /old_locus_tag="pgaptmp_002386"
     CDS             10431..11324
                     /gene="yidL"
                     /locus_tag="ID725_RS11930"
                     /old_locus_tag="pgaptmp_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGV7850975.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(11321..12643)
                     /locus_tag="ID725_RS11935"
                     /old_locus_tag="pgaptmp_002387"
     CDS             complement(11321..12643)
                     /locus_tag="ID725_RS11935"
                     /old_locus_tag="pgaptmp_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGV7850976.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(12643..14259)
                     /locus_tag="ID725_RS11940"
                     /old_locus_tag="pgaptmp_002388"
     CDS             complement(12643..14259)
                     /locus_tag="ID725_RS11940"
                     /old_locus_tag="pgaptmp_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGV7850977.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            14555..15271
                     /gene="yidP"
                     /locus_tag="ID725_RS11945"
                     /old_locus_tag="pgaptmp_002389"
     CDS             14555..15271
                     /gene="yidP"
                     /locus_tag="ID725_RS11945"
                     /old_locus_tag="pgaptmp_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGV7850978.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            complement(15268..16929)
                     /gene="yidE"
                     /locus_tag="ID725_RS11950"
                     /old_locus_tag="pgaptmp_002390"
     CDS             complement(15268..16929)
                     /gene="yidE"
                     /locus_tag="ID725_RS11950"
                     /old_locus_tag="pgaptmp_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGV7850979.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(17125..17553)
                     /gene="ibpB"
                     /locus_tag="ID725_RS11955"
                     /old_locus_tag="pgaptmp_002391"
     CDS             complement(17125..17553)
                     /gene="ibpB"
                     /locus_tag="ID725_RS11955"
                     /old_locus_tag="pgaptmp_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGV7850980.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(17665..18078)
                     /gene="ibpA"
                     /locus_tag="ID725_RS11960"
                     /old_locus_tag="pgaptmp_002392"
     CDS             complement(17665..18078)
                     /gene="ibpA"
                     /locus_tag="ID725_RS11960"
                     /old_locus_tag="pgaptmp_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGV7850981.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(18072..18144)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            18384..18716
                     /gene="yidQ"
                     /locus_tag="ID725_RS11965"
                     /old_locus_tag="pgaptmp_002393"
     CDS             18384..18716
                     /gene="yidQ"
                     /locus_tag="ID725_RS11965"
                     /old_locus_tag="pgaptmp_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGV7850982.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(18718..19932)
                     /gene="yidR"
                     /locus_tag="ID725_RS11970"
                     /old_locus_tag="pgaptmp_002394"
     CDS             complement(18718..19932)
                     /gene="yidR"
                     /locus_tag="ID725_RS11970"
                     /old_locus_tag="pgaptmp_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGV7850983.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            20033..21097
                     /gene="cbrA"
                     /locus_tag="ID725_RS11975"
                     /old_locus_tag="pgaptmp_002395"
     CDS             20033..21097
                     /gene="cbrA"
                     /locus_tag="ID725_RS11975"
                     /old_locus_tag="pgaptmp_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGV7850984.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(21094..21207)
                     /locus_tag="ID725_RS11980"
                     /old_locus_tag="pgaptmp_002396"
                     /pseudo
     CDS             complement(21094..21207)
                     /locus_tag="ID725_RS11980"
                     /old_locus_tag="pgaptmp_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418146.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21343..21999
                     /gene="yidX"
                     /locus_tag="ID725_RS11985"
                     /old_locus_tag="pgaptmp_002397"
     CDS             21343..21999
                     /gene="yidX"
                     /locus_tag="ID725_RS11985"
                     /old_locus_tag="pgaptmp_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGV7850985.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(22045..22857)
                     /gene="yidA"
                     /locus_tag="ID725_RS11990"
                     /old_locus_tag="pgaptmp_002398"
     CDS             complement(22045..22857)
                     /gene="yidA"
                     /locus_tag="ID725_RS11990"
                     /old_locus_tag="pgaptmp_002398"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGV7850986.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(22972..23370)
                     /gene="yidB"
                     /locus_tag="ID725_RS11995"
                     /old_locus_tag="pgaptmp_002399"
     CDS             complement(22972..23370)
                     /gene="yidB"
                     /locus_tag="ID725_RS11995"
                     /old_locus_tag="pgaptmp_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGV7850987.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            23488..23586
                     /locus_tag="ID725_RS12000"
                     /old_locus_tag="pgaptmp_002400"
                     /pseudo
     CDS             23488..23586
                     /locus_tag="ID725_RS12000"
                     /old_locus_tag="pgaptmp_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23610..26024)
                     /gene="gyrB"
                     /locus_tag="ID725_RS12005"
                     /old_locus_tag="pgaptmp_002401"
     CDS             complement(23610..26024)
                     /gene="gyrB"
                     /locus_tag="ID725_RS12005"
                     /old_locus_tag="pgaptmp_002401"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGV7850988.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(26053..27126)
                     /gene="recF"
                     /locus_tag="ID725_RS12010"
                     /old_locus_tag="pgaptmp_002402"
     CDS             complement(26053..27126)
                     /gene="recF"
                     /locus_tag="ID725_RS12010"
                     /old_locus_tag="pgaptmp_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGV7850989.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(27126..28226)
                     /gene="dnaN"
                     /locus_tag="ID725_RS12015"
                     /old_locus_tag="pgaptmp_002403"
     CDS             complement(27126..28226)
                     /gene="dnaN"
                     /locus_tag="ID725_RS12015"
                     /old_locus_tag="pgaptmp_002403"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGV7850990.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(28231..29634)
                     /gene="dnaA"
                     /locus_tag="ID725_RS12020"
                     /old_locus_tag="pgaptmp_002404"
     CDS             complement(28231..29634)
                     /gene="dnaA"
                     /locus_tag="ID725_RS12020"
                     /old_locus_tag="pgaptmp_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGV7850991.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            29931..30011
                     /locus_tag="ID725_RS12025"
                     /old_locus_tag="pgaptmp_002405"
     CDS             29931..30011
                     /locus_tag="ID725_RS12025"
                     /old_locus_tag="pgaptmp_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7850992.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            30241..30381
                     /gene="rpmH"
                     /locus_tag="ID725_RS12030"
                     /old_locus_tag="pgaptmp_002406"
     CDS             30241..30381
                     /gene="rpmH"
                     /locus_tag="ID725_RS12030"
                     /old_locus_tag="pgaptmp_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGV7850993.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            30398..30757
                     /gene="rnpA"
                     /locus_tag="ID725_RS12035"
                     /old_locus_tag="pgaptmp_002407"
     CDS             30398..30757
                     /gene="rnpA"
                     /locus_tag="ID725_RS12035"
                     /old_locus_tag="pgaptmp_002407"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGV7850994.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            30721..30978
                     /gene="yidD"
                     /locus_tag="ID725_RS12040"
                     /old_locus_tag="pgaptmp_002408"
     CDS             30721..30978
                     /gene="yidD"
                     /locus_tag="ID725_RS12040"
                     /old_locus_tag="pgaptmp_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGV7850995.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            30981..32627
                     /gene="yidC"
                     /locus_tag="ID725_RS12045"
                     /old_locus_tag="pgaptmp_002409"
     CDS             30981..32627
                     /gene="yidC"
                     /locus_tag="ID725_RS12045"
                     /old_locus_tag="pgaptmp_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGV7850996.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            32733..34097
                     /gene="mnmE"
                     /locus_tag="ID725_RS12050"
                     /old_locus_tag="pgaptmp_002410"
     CDS             32733..34097
                     /gene="mnmE"
                     /locus_tag="ID725_RS12050"
                     /old_locus_tag="pgaptmp_002410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGV7850997.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(34247..34884)
                     /locus_tag="ID725_RS12055"
                     /old_locus_tag="pgaptmp_002411"
                     /pseudo
     CDS             complement(34247..34884)
                     /locus_tag="ID725_RS12055"
                     /old_locus_tag="pgaptmp_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
BASE COUNT         8445 a         9197 c         8770 g         8472 t
ORIGIN      
        1 acccttttgt tgactttgtt cttttatagt tcaatgagca tccataatca tggaatcaaa
       61 aaaaacatca gttcttttaa aactttcctg aacaggtaca tacccttcat cttttttaat
      121 tcgcagtttt aataaatcat atatatatta cttaaaattc ctgcaggaat ttcgtaatta
      181 aaatccgtat tcttcataac aatactatta actaatttct tcctcaaaac cgtgtcagcg
      241 gaatgtgttt ctaacgcaga agttttttca gacaacgatt taataatagt cgctacagcc
      301 actcctgaat atggaatttc ttttcgtgaa ggtttatctc tgccaaccag tgtaatatta
      361 aaagacttat cacctgtttg tttgatttcg atatattcat cctctccagt tgccgccacc
      421 caggtacgat taccattatt ttccgtgcct ggcatagggt ggaaatagtc aagaatatcg
      481 gtgttaatac tccgagaacc ggacatgatg tttagtatta agcgctttat ttcctgcaac
      541 atccattttg cgaaattact gccaggtgaa tattttttct cgttattttt aatatcaagc
      601 tcagcagcga catttctatt tttatccaaa aaggtggaag ggcagagatt tgccatcatt
      661 attccgctgt ttataggact tgccataata tacctcacga tgaaccatta attaatagaa
      721 tgctggtcat gaccactggt atagacagca atcagaattt aacaccatcg ctgaaaattt
      781 tagccctaac tgtccccccc aggttaccgg tgctgcgact taaattaacg tcctgggcta
      841 ataaattgtg cctgagatgg tctctggcac aatttcaccc tatatatctg cacggatcta
      901 tcaaaaatcg ctcatcagta actcccccga gcggtttaat gtcgctcctt actgaaaaaa
      961 cactgcaatc ttgttaaaca tcgtcggatc ggactgatta cgctgcactt tcaccacatc
     1021 ttccagctta tcgatttggc ttatcatctg ctccagacgc tggtcgtcat tgaccagtag
     1081 ccagatatgg cttttgtcgc tgtcctgaat cggcagacaa agaatgcctt caacgttaaa
     1141 agcgcggcgg gcaaaaaggc cacaaacgtg ggtcattacg cccggatggt tgcgaacggt
     1201 gagttccaga atcacgttgt catgagttgt gttttgcatg gcttattccc ccaccatttc
     1261 tgtattcgcc gcacctggcg gcaccatcgg gtacactttt tcttcggcat caatgcgcac
     1321 atggatcagc gccggaccag ggcgattgat gatttcctgc aatgcagcct gcggatcggc
     1381 ttcgttattc aaatcacagg tttcgaggcc gaatccggcg gcaatctgca taaagttgat
     1441 ttttcccgga taggtagcgg caaaaacgcc ctgcttgtag aacagactct gttgctgatg
     1501 caccagcccc agcgcttcgt tgttcatcag aatgatttta acatccagct ggttttcgct
     1561 ggcggtcgcc atctcctgaa tattcatcat caggctgcca tcgccggaga aacacaacac
     1621 tttgcgatcc gggttcgcca gcgccgcgcc aatcgccgca ggcaggccaa aacccatcgt
     1681 gcccagcccg ccggaggtca gccactggcg tgggcgattg agcggataag cctgcgccgt
     1741 ccacatctga tgctgaccca catcggtggt gataatcgca ttgtcatcga cacaggcggc
     1801 aacggcgttg atcaggccgt aatggcttaa cggatcgcac gctttcggga ttggacacgg
     1861 gaattcgcgc tgcaaatccg ctaccaactg gtgccactct gcacgcggtt gcgcttccac
     1921 ctgcgggatc agctgcgcca gcacgtcatc aacatccgcc tgaatcgcta cgtgcggctg
     1981 cttgatttta cccagctctg cgcggtcgat atcgacatga ataattttgg cattcgggca
     2041 gaactgctcg gttttgccaa tcgcccggtc atcaaaacgc gcaccgagca caatcaacaa
     2101 atccgcctcc tgcaaaatat agttggtgct gcgcacaccg tgcatcccca acatacccag
     2161 cgacaacgga tgcgcttttg gcaacatgcc cagcgccatt aaagtcattg tggtaggcag
     2221 ctgcgctttc tccgccagtt cacgcactct cgcaggcgca ttgatcacac caccgcccag
     2281 ataaagcacc gggcgtttgg cagcattaat cattgccgct gcgtcacgaa tgctttcttc
     2341 gctaaaggcg ggcgcgacgg ctttttctgc caccgcgggc tgtgcttcaa tctcaaaaac
     2401 ggccgtttgc acatccttag gaatgtctat ccacaccggg ccagggcggc ctgattgcgc
     2461 aatacggaag gcatcgctca tgacctgcgg gagttcttcg atatgtctga ccagatagtt
     2521 gtgtttggtg atggggatag agatgccgta ggtgtcgact tcctggaagg cgtcagtgcc
     2581 gatcattgag gcgggaacct gaccagtgat gcaaatcagc gggatggagt ccagccgcgc
     2641 atcagcaatg gcggtcacca ggttagtcgc ccccggtccg ctacaggcca tacagaccgc
     2701 cggtttaccg tcggtgcgcg ccattccctg agcgataaac cccgcgccct gttcatggcg
     2761 agccagaata tggcggattt gcgtgctttg gcttaaggca tcgtaaacag gcaggataga
     2821 accgcccgga atgcccgtca caatcttaat gccctgctgt tccaggaaat gaacgataaa
     2881 ttctgcgccg gtaaagcgct tacgcgtcga tgatgtgccc gaacttgcca tgctccagtc
     2941 cttttcttct gggccgactt tccgggaggt gcttaaacga aaaaccccgc ccggtttgcg
     3001 ccggcggggt tttggaatcg tgtgttgttc cagtccctac ggcgcattgc cgacgaccac
     3061 caccacacgc acgacgacca ctgcggcgga tggcgcagtt ggtagtagtt ttgcgttgag
     3121 catggaagta gccattgggg accttgttgg ttttgtgttt aacaatattt atacaagcac
     3181 agctttacag gggagacaat ggaaaatttt tcagcaaggg aaaattgagg ggttgatcac
     3241 gttttgtact gtactgcagg taacaaaaaa ccccgccgga gcgaggtttc gtcagccgcc
     3301 cgcggctggt aaccgcaaag cacactgtat tatgtcaaca cagaaagtat acgtgttccg
     3361 cgcaaaacgc gcaatttcgg cacgaatttt gacgtattta gtgcatagtt gagtatcgat
     3421 cacagtttgc gttttgtcca aatattactg tttatttata cagcaagctt ctataatatc
     3481 actgtacgca atgtgttatg cgggggccgc atcgttaccc ggcgcactaa gtcctggctg
     3541 aaacgggtgg tgccgtcagc gccttaaccc cgcgtgagca cactgtgtta tgtcaacgag
     3601 cacaacgttt ctccttgaga taccgcgtgc acaacagctg gcaacaggca gcggaaaggt
     3661 acgtcagctg gcagtgctcc tgaaccacag gagacgcgta tgagcctggt ggatatcgcc
     3721 attcttatcc tcaaactcat tgttgcagca ctgcaactgc ttgatgctgt tctgaaatac
     3781 ctgaagtaat tcagattcaa gtcgcactaa aggggagcgg gtaaccgctc ccattttata
     3841 tttagcgtgt gggttggtgt cggatgcgat gctgacgcat cttatccgac ctaccatccc
     3901 tcccggcaac atttattgcc gcttttgttt acatattctg ccgctaaaca attccccatt
     3961 cctggcgtat atctggctaa catttatcaa tatgatagat tcctctcccg catttatggg
     4021 aatgcgtagt gacttattct aattattttt ataaaagcat ccgtgataat gaaaaggcat
     4081 agaaacatca atttgttatt gatgttggta ttactcgtgg ccgtcggtca gatggcgcaa
     4141 accatttata ttccagctat tgccgatatg gcgcgcgatc tcaacgttcg tgaaggggcg
     4201 gtgcagagcg taatgggcgc ttatctgctg acttacggtg tctcacagct gttttatggc
     4261 ccgatttccg accgtgtggg tcgccgaccg gtgatcctcg tcggaatgtc catttttatg
     4321 ctggcaacgc tggtcgcggt cacgacctcc agtttgacag tattgattgc cgccagcgcg
     4381 atgcagggga tgggcaccgg cgttggcggc gtaatggcgc gtactttgcc gcgtgattta
     4441 tatgaacgga cacagttgcg ccacgctaac agcctgttaa acatgggaat tcttgtcagt
     4501 ccgttgctcg caccgctaat cggcggtctg ctggatacga tgtggaactg gcgcgcctgt
     4561 tatctctttt tgttggtact ttgtgccggt gtgaccttca gtatggcccg ctggatgccg
     4621 gaaacgcgtc cggtcgacgc accgcgcacg cgcctgctta ccagttataa aacgcttttc
     4681 ggtaacagcg gttttaactg ttatttgctg atgctgattg gcggtctggc cgggattgcc
     4741 gcctttgaag cctgctccgg cgtgctgatg ggcgcggtgt tagggctgag cagtatgacg
     4801 gtcagtattt tgtttattct gccgattccg gcggcatttt ttggggcatg gtttgccgga
     4861 cgtcctaata aacgcttctc aacgttgatg tggcagtcgg ttatctgctg cctgctggct
     4921 ggcttactga tgtggatccc cgactggttt ggcgtgatga atgtctggac gctgctcgtt
     4981 cccgccgcgc tgttcttttt cggtgccggg atgctgtttc cgctggcgac cagcggcgcg
     5041 atggagccgt tccctttcct ggcgggcacg gctggcgcgc tggtcggcgg tctacaaaac
     5101 attggttccg gcgtgctggc gtcgctctct gcgatgttgc cgcaaaccgg tcagggcagc
     5161 ctggggttgt tgatgacctt aatgggattg ttgatcgtgc tgtgctggct accgctggcg
     5221 acgcggatgt cgcatcaggg gcagcccgtt taagctcacg tcaccgcagc atcgtcatca
     5281 gctccatggg agaacgatgc tgctttatca gatcacgcat cacccgcata tgcggcgcag
     5341 agtaagaata aaacgcctga tagcccgcac aaagcacgct gtctggcgtc tcctcccgag
     5401 cgtgcggaca cagcagatgc cagtcgcacg cctggcagcc acggcgcggt tgcgggtttt
     5461 cgctgccaca ccatttttgt aatgtggcgt caaaaagctc aatcgagata cgactgatat
     5521 ctgcccgcac ccaacactca aacagggcaa ttaaaaattt cccccacagc tctgctgtca
     5581 ccgaagcggc cgtcatatcg ccccggtcat caaattcaag atggggaata agctgtacgg
     5641 agcggatgcc ccgttgtttg aaattgtgat aaaaatcggc aggttgcaga gcatccttgt
     5701 actctacaac cagtttatgc ctgtcctctt cagcatcaat gacctgactt cctgtcatgc
     5761 gactctctca ataaatacga taagttgatg tatatgcgtt acagcaaaca gtattgcgag
     5821 agataacacc gcgagggaga tcaaaaattt gtcacggact acgtgaaatt gggaaaaatc
     5881 gctattcgtg acatccatta aattgcgatt acgagtgtag tgccagagga tcagcgccac
     5941 gatggcgagg atgccaatcg aaatccagaa taacatgccc gcctggtgcc agttgtgttt
     6001 gatagccaac gccatcaacg cgccgtagcc cagcatggta cgaaaccacg ccagtgatgt
     6061 acgctccggc tgcagccctg ggtcggcaat gcgtctggct ttgcggctat ccggcataca
     6121 acaccagtcc cataacgatc actgctacga ccatcaatat taagctgatg attaataagc
     6181 tgttggtgta gggcaggtcc tctttcagac gcatcgcttt ttcattgcgt aaccagcgca
     6241 agtagccata catcgctaac ccgccggaaa acagacacaa caacaacgcc agcagttcgc
     6301 gaatgacggg cgtggcgaaa tctggcgcaa gttgatcaag cccaaccccc gccgccagaa
     6361 atcctagtgc agtacggatc cacgccagaa aagtacgctc attagccagc gagaagcggt
     6421 aatcaggcgc ttctccgagg cgggaaattt tcattgttgc tcctttgcaa tttcactaac
     6481 gctctacctt tagcggtttt ttttaagaat ttcgtccgcc cgtttcctga tatgcttttg
     6541 ccatccgcta caaggaggtc agatgggtat catagcgcaa aataaaatca gttcattggg
     6601 catgctattc ggtgccatcg ctttaatgat ggggattatc cacttttcat tcggcccttt
     6661 ttccgcacca ccgccaacgc ttgaaagcat cgtggcggac aaaacggctg aaattaaaag
     6721 aggtcttctc gccggaataa aaggcgaaaa aattaccacc gtggagagaa aagaggacgt
     6781 ggatgtcgat aaaatcctcg atcaaagcgg tatcgcactg gccattgctg cgctactttg
     6841 cgcatttatt ggcggtatgc gtaaggaaaa tcgctggggc attcgcggcg cgcgggtttt
     6901 tggcggcggg acgctggctt tccatacctt gctttttggt atcggcatcg tgtgcagcat
     6961 tttgctgata ttccttatct tttccttcct gactggcggt tcactggttt aagcagcctg
     7021 aataatgccc gccgggtgtt catcagcggg cattatcgtc agaacttctg ttttttctct
     7081 tccaccttca ccccttgtgt cggcaggccg gtgtcatagt cgcgtaccac tggcgaataa
     7141 ccatcttgtg ggcgtggacg aaatgccccc atccagcgcg gctgtgcatc tttacgccac
     7201 ggacgcaggc tccattggta actgcggaac ggatcgcgaa ttttgtccat ataatccaat
     7261 aaggcatcat gcattttgct gcgaacgtcg gcaaaatggg catcatcgat caggttatgc
     7321 atttcatttg gatcattacg gcgatcgtaa agttcatcac tggtgaagag gttgagcacc
     7381 agtttaaagt catccgtcac ccagcaacgc accggaataa aaccgccaaa gctgtcatgc
     7441 tcaatctcgt agcggttaaa ttccaccatc acgccgcgtg gctctttcac tgcaaggata
     7501 ttttcccccg gcagaatctc tggtttttca atatctgcca gcgccatcat tgtcggcagt
     7561 aaatcgatat gactgactgg cgtatcgacc tgtcgccgct ccccttgcgg cgaacggatg
     7621 atcagcggaa tgcgggtgat gtcgtcatac atcgccgccc ctttactgat cagcttatgt
     7681 gcgcccatca tttcgccgtg atcggaggta taaataaccc acgtattttc acgttgctct
     7741 ggctttaagg cattgatgac ccgtccgatt tggtcatcaa caaagtcatt acaggcaaaa
     7801 tagagcggat ggtgataaag cccgtcatca ccgactggcg atggcatcgc ctgcgcccat
     7861 aagcggtgat gttctggttt gttcgccagg tcatcctgtg ctttctcgcc cagctcgtag
     7921 taaaaatcag cgtatttctc taaatactcc accggacagg tgaacgggtg atgcggctca
     7981 tcatacgaaa ccaccatcag gaagggttcc tcggcgcgcg cgggctgttg cagaaaatcc
     8041 accgctcgat tgctgatgcg gtgcgcccag gtgaaggttt cgtcaatatg gttcgcctgt
     8101 aaatcttcga cgctgtttag gccattgcgc cacaggctaa tctctttttc cgtcagttcg
     8161 ctaagatagt tcgccccatc gaaccagtaa tcagcgtccc actccggcgg acactcgcca
     8221 gtgccgaaat agtcatgacc gtcgagatgc catttgccga tgtaacaggt gtgatagccg
     8281 gcatccttaa agtagcgccc catagtggag atgtttttac ccggcgcgac gttgttggtc
     8341 cacggcccgg actggttagc gtagataccg gtaaacagcc cggcgcgcgc cggtgtacaa
     8401 accggtgaac aggtgtaggc ggaattaaag cgaatacctt ccgccgccag actatcaata
     8461 ttttgcgtat tcagcggttt accgctatag caaccgacca tattgggggc ctgggtatcg
     8521 gtcatgatga acagaaaatt ggggcgtttc ataggctatc cttcgcgtcg ggagtaaagg
     8581 ttacggctgg gtcgtgacga tgccgccagc tgtcaaaaat caggtagaca atcactacgg
     8641 cggcaatgaa ataactgatc atcgctaacc agcgcgtacc gtagccgccg aattcagcca
     8701 gcccggcata gacgccaatc atcgcgaaca ggatgccaat tgacgcgatc ttgacgtttt
     8761 tccacggttt catgtccacc gcaaacgcat ctttgaaggt gaacggcgtg gcgcgcggtt
     8821 tgataaaacc gatcaccagc atcacgacca cgttgatgca gaacgtacag gccagcacat
     8881 agaggaaatg gaagtcgaac ttcaccagat agttgatggt gatgtagctg attatgccaa
     8941 tccccatcgc cacttttgcc gccagcgccg ggatgcgcag gaagaaaaag cccataatga
     9001 tgatggtaac cagcggcacg ttgtaaatgc cgttgagctg tttcatccag ctatacagcc
     9061 cctgcggcgc gttggcgatc cacggcgcta ccagcaccga aacgatagcg ataaagaaac
     9121 caaatttgcg cccgacggtg accagctgct gcggctcggc attctggtta atgatgcgac
     9181 ggtaaatacc catactgaat aacgtactgg cgctattcag aaagccgttg aaggtactga
     9241 tcactgcacc aaataacacc gcgccgaaga aacccaccag tggcactggc agaacgttat
     9301 tgaccagcgt cgggtaggcc atgtcggctt tcggtagatc ctgatacaga tgaaacgcaa
     9361 tcaaccctgg cagcaccagt accagcgggt cgagcatttt cagcaccgcc gttaacagcg
     9421 cccccttttg cccttccgcc aggctttttg acgccagcgt gcgttgcacg atgccctgat
     9481 ttgtacacca gtaaaaggtg ttcaccagaa ttaaaccggt aaatgccgcg ccaatcggca
     9541 agggatcggt cgggccaccg actgagttta atttctcggc gtgaacggtg gtaatttgct
     9601 caatgccctg cataaagctg cccttgccca tcgcgatcaa gccaaatacc ggcaccatca
     9661 acccgccgat aaccagccca ataccgttga tggagtccgc cactgccatt gcgcgcagtc
     9721 cgccgatcac cgcatacaaa attcccgcca gaccaagcaa aattaccagt agccagatag
     9781 ccgcaccgtg agaaatctgt agcgattccc cgacgtgaaa caggctgttg agcgccaacg
     9841 cgccggagta gagaacaatc ggcagaaagc agacgccggt ggcaatgagg aagcagaagt
     9901 cgatgatcat acgcgtcgtt ttatcataac gttcctccag aaaatcgggg atggtggcaa
     9961 tgccgcgctt cagatagcgc ggtaaaaaga ttagcgcgag gaagatcagc gtcaccgctg
    10021 aagtcacttc ccagcccatc accgacatgc cgcttttgta ggcctgcccg gaaagaccaa
    10081 ccagttgttc cgtggaaagg ttggttaaca ttaacgaagc ggcaataacc ggcgctttta
    10141 gtgaacgtcc ggcaagaaaa tagccttgtt gcgatccggt atctgttttg cgaaccttcc
    10201 accaggtgat gatcgccacc agcagcgtaa aaccgacaaa actcaagatt tgtaacgaat
    10261 tcatctcata gcccttattt atgtttatgt cccggacgca tgaatatagg gagaggtaaa
    10321 atgcgtcggg tcaggcagtc agattcttaa aatacaaacg tcgtatccct gaacggattg
    10381 ttttctgtta agttcaggtt gtgagcataa ttcgccggag ggatattaaa atgaatggaa
    10441 aattgcaaac ttcagatgta aaaaacgaaa ctccatacaa tattccttta ttgattaacg
    10501 aaaatgtgat ctccagcgga atttctctga tctcgctctg gcatacctac gccgacgagc
    10561 attaccgggt gatctggcca cgggacaaga agaaaccgct tatcgccaac tcatgggttg
    10621 cggtgtatac cgtacaagga tgtgggaaga ttcttttaaa gaatggcgaa caaataacac
    10681 tgcatggcaa ctgtattata tttttaaagc caatggatat tcactcttat cactgtgaag
    10741 gtttagtctg ggaacaatac tggatggaat ttacccccac cagtatgatg gatattccca
    10801 tcggtcagca aagcgttatt tacaatggcg aaatttataa tcaggaactc accgaagttg
    10861 ctgagttaat aacttcacca gaagcaataa aaaataatct ggcagtcgct tttctgacga
    10921 aaattattta tcagtggatt tgtcttatgt acgcagacgg taaaaaagat ccacaacggc
    10981 ggcaaattga aaaattaatt gccactttac atgccagtct gcaacaacgc tggagcgtag
    11041 ccgatatggc tgccacgatc ccctgtagcg aagcctggtt gcgtcgtctg tttttacgct
    11101 ataccggaaa gacgccgaaa gaatattacc tcgatgcgcg tctggatctg gcgctatcgc
    11161 tattaaagca acaaggaaac tcggttggcg aagtcgctga tacgctcaac ttcttcgact
    11221 cctttcattt cagcaaagcc tttaaacata aatttggtta tgcgccttca gccgtgctaa
    11281 agaatacgga ccagcaccca acggatgcca gtccacacaa ttaacgtaac tctggccagt
    11341 acgctttgtt ggcctccagc aactcatcca gaatagcttt ggcgaccgag gcgctcggta
    11401 ccgttttcga caacgtaatc gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt
    11461 ccacgaccag tttttccacc gccacttgct gactcattaa ccctttctga aactgcggga
    11521 tatcgccgac caccagtggt tctggtccgt tgtgcccgac cagacacggg atctcgacca
    11581 tagcttcatc atcaaagtta tggattgccc cgttgttagg cacaatcagc aacatccgct
    11641 cctgagtgtt gaaggcaatt gccgccgcca gatcgacgat gtatgacgca tgttcgtcaa
    11701 tttccagctt gcccgccgct gaatttcctg ccgccgtaat ggcgcggcaa gcatcgaaaa
    11761 cctgtttctc gcgatgttcc attacctcat tcgcgcgagt atgttcaggg ttggaatgct
    11821 gaacgacata atccgggaag agataatatt tcagataggt gttcggcaat gtatccgggt
    11881 ccagtgcctg gacatcccgc gcttttgcat aggtgtcgtt ccagctcgct tcagtatgtg
    11941 tatcttgctg cggtggaaca taaccgtgct tagagacata ttgccgcagc tggtgcatca
    12001 ggtcgttgcc ctgcaaatct tcaattgatg tccaccagcc aaagtggttc aggccgtagt
    12061 agcgcacgcg catctgtttg cgatcttgca gcccaacaat ttgcgccatc cggctttcaa
    12121 taccgattgg catgtcacag atgttgagga ttttcgcatt cgggcgcaga cgacgcgtgg
    12181 cttctgcaac aattgctgcc gggttggagt agttgagcat ccaggcgttt ggtgaatatt
    12241 tttccatata atccaccagt tccaggacgc cgccaatgga acgcatgccg tacgctattc
    12301 cgccgggtcc gcaagtttcc tgaccaacaa cgccgtggcg cagcgggatt ttttcatcca
    12361 gttcgcgcat cgggtatttg ccgacgcgga tgtgcgccat gacaaaatca acgtcgctga
    12421 acgccacttc aggatcggtg gtgtaactaa acgcaatatc cggcgctttt tctttaagga
    12481 tgactttaca ggcttcggca atcacttcct gccgcgaacc atcgttatca taaaatttca
    12541 gtgcacgaag cgggaaacgg tcctgattcg ccaggagcat caacacgatg cctggcgtaa
    12601 aggtgcttcc accgcctgcg acaaccactg agaatttggt cattatactg cctccgtaat
    12661 ggcaacattt tctgctgatt gatgagaatt aattaagcta tcgagctgtt cacgcagctg
    12721 ggatacatgc agaccaatga tcacctgaat ggcatcgcca ctacggaaga cgccgtgcgc
    12781 tcccagcttt ttaaagactt cgtcatccag cgtttgtgac atgtcatgca gtgcaatgcg
    12841 tagacgcgtc gcacaattgt taatgctgga gatattgccg accccgccca gggcttgcag
    12901 gataccggca gcctgatcca gctctttttt tggctctgcc gcggtggttt ggcctcgcga
    12961 ggctttgtat tcggcttttg agtagagttt cacttccgca tcttcacgtc ccggcgtgca
    13021 catgttgaat tgcagaatca gggtgcggaa aaccacgaag tacagcaggg taaagcacaa
    13081 cccaatggcg atttgggtca gcatcatatc cgcgtggttg ctgaacatcg ggatccagtt
    13141 ttgcggtaaa acctggtcaa tcagacctcc gcccatgttg cccaccacac caaagagata
    13201 catcacggtc gacattgagg ccgcaagcac agcgtgtacc gcaaacagca acggtgaaat
    13261 gaacaggaag gtaaattcca gcggttcggt aattcccacc agcatggcgg ttaaggttgc
    13321 gggaatcagc aaacccgcca cttttacccg attttccggt gcggcagtga agtacatcgc
    13381 taaagaaatg cccacggcac caaagatttt tgagttaccg tgcagggcaa aacctccttc
    13441 cgggaacaac gatttcagcg gctcggcgct caaactgaac tcttgcagat gctgcgccca
    13501 gtacatctga attccgcctt caacagctgc cggaccaaag ataaacggtc cgtagatgaa
    13561 gtggtgtaaa ccggttggga tcagaatacg ttcgaggaag gtgtaaaccc acaccccaag
    13621 cgcacccgcc gaacgcagga acgcttgcag agattcaatc cccatttgta cttttggcca
    13681 gccgagcaat gtcagccagg cacaggggat catcaccagg aaggcgataa tcaccacata
    13741 agacgtcccc tggaaaatgc cgagaaaaac cggcagtttt ttatcgaaca gacggttatg
    13801 cagcgccgtc acaatgccgg aaatgataat tgcgccgata atgctggtat cgagggtttt
    13861 aatcccggcc atcattgtca gaccgctacc tgccaccgcg tcctgagtga aatcgacgcc
    13921 gaagtagctt ccccaggtca ttcccatcgc attgatgaaa tagttccagg tcaggaaact
    13981 caccatcacc gccagacaag cacgcccctg cgcttgctta gcaaggccaa tgggtaaacc
    14041 gacagcaaaa atcagcggca tattacggaa taccgtccaa ccgccctctt caataatgtg
    14101 tacgatttgc gcgaataaac tgttcggatc ggtcagtgat tccccgacaa acatcgggtt
    14161 ttgcagcaag atggcaagac ccaccacaat cccggcgaag ggaaacagca gcactggcgt
    14221 gaacatcgcg ccgccaaagc gttgaatttg actgagcatt tccacatcct cttttctcaa
    14281 ttctgtaagt aggaagcctg attggttatg cggcaggcct ggagcgagga taggaagatg
    14341 cgatcggcag aacatgacga cccaatggga ttcgtgatcg ctttcatgct tttcgttggg
    14401 cggtagagat ctactttttc gcgaaaaagt agatctctgt gagcactggt acacagcatc
    14461 gaacacatct ttaaaaaaaa gatgtttttt caattgatta agcagaactt gtgggcgcat
    14521 tacccgggct tgcaggcaaa aaagagatct agagatgatc tacaaaagca ttgcggagcg
    14581 gttaagaatt cgacttaatt ccgcggattt cacgctaaac agccttcttc ccggtgaaaa
    14641 aaagctggcg gaagagtttg cggtatcgcg gatgaccatc cgtaaagcga ttgacctgct
    14701 ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga
    14761 tgtgctgcat caaaccgcca gcctgaccgg gctggtggag gtgttaaaac ggcagggaaa
    14821 aacagtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg caattgccag
    14881 ccagttgcgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga
    14941 agggaaaccg ctgatgctgg aagacagcta tatgccggta aaactgttcc gtaacctttc
    15001 gctgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat
    15061 tggcggtaat tatgaaagcc tgacgccggt gctcgccgat agactgctgg cgcggcaaat
    15121 gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg
    15181 ggagtttttg aattattcag tgatgttcag aaatgccagc gaataccagg tggagtacca
    15241 tttacggcga ctccacccgg acaagagtta accgatactc cagaagagca ccgccagtaa
    15301 ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc
    15361 cgccgcaccg ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat
    15421 ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag
    15481 aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag
    15541 cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac caacgaccga
    15601 gaggaacagc acgatcccca gttcccgcag cgcgaggttg gcacttggcg gcataaacca
    15661 gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc
    15721 cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa
    15781 caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg
    15841 ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt
    15901 gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg
    15961 gttcaggcgc gagataacaa cgtcatagcg ttctttaaaa tgcaggtcgc gaatacgctt
    16021 accaagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt
    16081 ggacagcgaa gtatcgacct cctgaccaat caccagttgc gcattatgta aatccgctgg
    16141 ctgacccacc agatgcagca aatcgcccag ttggataatg gtatctggcg aaggaacttt
    16201 tagggtttct tcgcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac
    16261 atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat
    16321 cagcgcgccg ccattggtgc gtgaagactc gtgctgctgg gcttctgtct cgacattgac
    16381 gcggaaaata acccgcaaca tccacatggt gaacaatatc ccgcaaatgc cgaatggata
    16441 cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg
    16501 caaaatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg agaaaatccc
    16561 cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc
    16621 gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc
    16681 aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaattc
    16741 ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc
    16801 gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac
    16861 gttgccgata aacaaaccga cgactgccac caaagccaaa atactgaccg ttaatgctat
    16921 atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat
    16981 cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc
    17041 ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa
    17101 tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat
    17161 acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt
    17221 tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaagctca ggctaaatgg
    17281 ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc
    17341 ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac ggaaacctgc
    17401 cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa
    17461 gctctgactt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg
    17521 acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt
    17581 aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag
    17641 gccgcgcaag gcggccttag gaaattagtt gatttcgata cggcgcggtt ttttcgcttc
    17701 cggaatcacg cgttcgagat cgatatacag caaaccattt actaggttag caccacgaac
    17761 atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta
    17821 cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc
    17881 ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta
    17941 atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg
    18001 gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga
    18061 taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca
    18121 caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcgg ctacgatgta
    18181 aaaatgggtc tggaaatgaa cttttcaagc cttatcagcg gacttttttt tgcagtttat
    18241 ggtctattgc atagactgag ggggcagcac gaaacgttaa agtgcgatag ccgccacaga
    18301 gcgacgtaat gatgatgtta ttttcagcaa gggatcgcta taactcatca ggccaacacc
    18361 cacgatcaac agatgaattt attatgataa gaaatgtgtt gttagcgttc atgatatgca
    18421 gcggaatggc attactcgga ggatgctcca gcgtgatgtc ccataccgga gggaaagaag
    18481 gaacgtatcc cggcacccgc gccagcgcca ccatgattgg cgatgatgaa accaactggg
    18541 gcactaagtc gctggcgatt ctcgatatgc cgtttaccgc tgtgatggac acgcttttat
    18601 taccgtggga tgtgttccgc aaagacagct cggtgcgctc acgcgttgaa aaaagcgaag
    18661 ccaacgcgca agccaccaat gcggttattc caccggccag aatgcctgat aactgattta
    18721 gcgatcagtt tcggtgatcc acagctggcc gccttccatc cacgccagcc actgaccatc
    18781 cggcgagaag accacggcgt ccgcagaagg tggattggcg tggttttcgg ttaaatactc
    18841 aacctcgccg ctttgcgcat gggcacaagc aattcgatta cccagcacaa agcccaacca
    18901 ttctcctgac ggatgccagt taaatgcaga ctgaatatcc gttttgttat gggttaactg
    18961 gcgcggctcg ccgccctgtg gcgagataag ccacagttgc acaatgccgt tatcatctcg
    19021 cattaaaaac gcgatttgcg taccctgcgg attacagcgc acccagtggc gcgggacgtt
    19081 gactaacccc ggataagccc gatggtgggt aaaggttaaa cgtcgctgca cgatgccacg
    19141 cggtggcgcg ggcagggttg tttccgttcc acttaacggc gcatcacctg ctgctttcca
    19201 gccagcttca tcttgcggta actcaacgat aaacagctcc ggcactttct cgccctttgg
    19261 cgaaagtgtg tcgccaataa acgccagcgc gtgatttcct acccatcctt cttcataagc
    19321 acgattgatt tcatcgctgc caggctgcgg cgtgggcgtg gttttactca ccagcacgca
    19381 ccagtggcta ccgctgtatt cacgcggatg ctgcttttgt acgttgaccg ggccaaacgg
    19441 tgcagcaacc ccgacgtttc gcaaatccag cgccgaatcg aactgatgca ttacatggtc
    19501 gttataggta aaactcacca tttcaccgtt cgggctaaag acatgcacat ggctgccgcc
    19561 acgcagcgcc cctgatgtgt acggaacggt aatatccatt gcatcgagat tgttcatctt
    19621 gccgccttca acaatcacgc cgcgacggtg atggaaatcg taatgccatg tttcatgagg
    19681 attttcaggt ccgtggataa agacatattt ctctgacttt ggatgaacgg tcaccacgcc
    19741 gacgtgcgcg ccctgtgaca cgcgatagat aacctcgacc tcgccggtat ggatattcac
    19801 acgctcaatg gtctcgccgg taaacgacgc gccagaagga cgcacgtcaa ataccagcca
    19861 ctggctgtcg ggcgtccagg tattggtatt ggtgagcagg tgattacggg gagcaaaggt
    19921 tatctgtttc atggcgcggt cctgatacaa aacggttccc gccatcatac ttcacaagga
    19981 cttcactttc agacaatagt cttttgccat cgtgatgaat gtgagtgaaa agatggaaca
    20041 tttcgacgtg gcgattattg gcctcggccc ggcagggtcg gcgttggcac gaaagttagc
    20101 cggcaaaatg caggtgatcg cgctggataa aaagcaccag catggtactg aaggtttcag
    20161 caagccttgt ggcggtctgc tggcaccgga cgcgcagcga tcttttattc gcgatggact
    20221 gacgcttcct gtcgatgtga tcgccaatcc acagattttc agcgtcaaaa ctgtcgacgt
    20281 cgccgcatcg ctcacgcgta actaccagcg aagctatatc aatattaatc gccatgcttt
    20341 cgacttgtgg atgaaatcgc tgatccccgc cagcgttgag gtttatcacg atagcctgtg
    20401 ccggaaaatc tggcgtgagg atgataaatg gcatgtcatt tttcgtgcag acggttggga
    20461 gcagcatatt actgcccgct atctggtcgg tgcagatggt gccaactcga tggtgcggcg
    20521 atatctctac ccggaccatc agattcgtaa atatgtcgct atccagcagt ggttcgcaga
    20581 gaaacatccg gtgccgttct actcatgcat ctttgataat gcgataactg actgttactc
    20641 atggagtatc agcaaagacg gttattttat ctttggcggt gcctatccaa tgaaagacgg
    20701 tcagacgcgt ttcacgacgc tgaaagagaa aatgagcgcc tttcaattcc agtttggtaa
    20761 ggcggtaaaa agcgaaaaat gcacggtgct atttccctca cgctggcagg attttgtctg
    20821 cggtaaggac aacgcctttc tgattggtga agcggcggga tttatcagcg ccagctcgct
    20881 ggaggggata agctatgcgc tggatagcgc agagattctg cgttcggtgt tactgaagca
    20941 gccagagaag atcaacgcag cctactggca cgccacccgc aaactgcgtt taaaactctt
    21001 cggcaagata gtaaaaagcc gatgcctgac cgcaccggct ttaagaaagt ggattatgcg
    21061 cagtggtatg gcgcatattc cacagttgaa agattagcca acgcgcgtca catcgcccac
    21121 cagcaggatg taagagagcg cgccaatcaa cgcgacggcg gagatataaa ccagtgcagg
    21181 tgcgaaccca tagccttgca acagatatta gtccacaaaa aaacacctca taagaaaagg
    21241 tcttatatcg ggcagaaatc tgaaaaggga gatcaggcgc taaacaggag ggaaagaaga
    21301 gtatgctttc aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaattttaag
    21361 ggatttttta aggctgccag tttattccca ctggcgctga tgctttcagg ctgtatctcg
    21421 tatgctctgg tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac
    21481 accatcactg ggctatcgca ggcaaaagat agtaatggaa caaaaggcta tgtttttgta
    21541 ggggaatcgc tggattacct tatcactgat ggtgccgatg acatcgttaa gatgctcaat
    21601 gatccagcac ttaaccggca caatattcag gttgccaatg acgcaagatt tgttttaaat
    21661 gcggggaaaa agaaatttac cggcacaata tcgctttact actactggaa taacgaagaa
    21721 gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg ttcaacactg taccaggtca
    21781 ctggaaaacc taaaaggcac aatccatgag aaaaataaaa acatggatta ctcaaaggtg
    21841 atggcgttct accatccatt taaagtgcga ttttatgaat actattcacc cagaggcatt
    21901 ccggatggtg tttccgcagc attactgcca gtgactgtta cgctggacat cattactgcg
    21961 ccgctgcaat ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt
    22021 ttatgctgtt tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac
    22081 gccatcttca aggttggatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc
    22141 attatccatt gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc
    22201 gccaatcgcc atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc
    22261 cgtacctttg ttaacgcgtt tatcaaggat ttcgaggaag tacggcgcac ttttcagcac
    22321 ggtatatttc tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg
    22381 ttcatcaatc atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca
    22441 gaacaccagc ggaatggtgg caacgaagga ttcatgcacc gtgtagtagc tgatatcacg
    22501 gttggcggtg tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga
    22561 gagtttttcc aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgttacc
    22621 atcagcggcc ttctgtacca gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc
    22681 catatgcagc tctttcaggt aattgtgaac acctgcataa gggcgacccg tcgttagcac
    22741 gacattcacg ccacgggcgc gagctgcggc aatcgcattt ttaacggcag gtgaaatggt
    22801 gtgatcgggc agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag
    22861 ttccccaggt agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca
    22921 acacccgagg gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa
    22981 gagtttccct ttcagcagtt ccatgcctgc ggaaagcaga tcgttattgg cttgtggtga
    23041 cacttcacct tgcggtgaga gcgcatcaat aatcttcggc aattgttctg ccagtaaaca
    23101 ggaagccgta ctggtatcca cgccaagttt ttgtccaaga tcggataccg cgtttgtgcc
    23161 gagtgctgat tccagttgct cgccgctaac tggttgattg ccctgttgat tactcagcca
    23221 ggttgagaga atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc
    23281 accctgctcc tcaacccaac ttaaaatcgc ctgatatttc cccgcatcgc ctttcagaaa
    23341 ggcgccgaca acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga
    23401 cccaaaaagt atagaactgc gggatgataa ttgcggattg cagaaataaa aagggcggag
    23461 atgatctccg cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc
    23521 ctacaaaaac atgcaaattc aatatattgc aggagtctcg taggcctgat aagcgtagcg
    23581 catcaggcac gctcgcattg ttaacgccat taaatatcaa tattcgccgc tttcagggcg
    23641 ttctcttcaa taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac
    23701 tggtcagcgg caatcgcatc cttaacggta acgcgcagca tacgacggct ttccgggtcc
    23761 atggtggttt cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg
    23821 gagaggccgc gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc
    23881 ggctgacgac gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc
    23941 tcacccagcg tgcagatacg acgatattcg ccaccagtga taaactcgtg atccagcgga
    24001 tagtcagtat ccacaccgtg ggtacgcacg cgaacaatcg gctcgaacag gttttgttcg
    24061 gcattggtgt ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg
    24121 ctgaccagcg cgttcaccca ccgggtaacg gtctgctcat cagagaggtc ggcttccgtc
    24181 agcgtcggct gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg
    24241 ttgatcattt tctgcgtcgc gttgtactca gacaccagtt tctctaacgc ttcgccagcc
    24301 aatgccggtg cgctggcgtt ggtgtgcagc gttgcgccgt ccagcgcgat agagatctgg
    24361 tactgatcca tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg
    24421 tacagcggcg gctgagcgat gtagacgtga ccgcgttcaa caatttccgg catctgacga
    24481 tagaagaagg tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg
    24541 atgatgatgc tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacaa
    24601 ccaagcgcgg tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct
    24661 ttctcgacgt tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc
    24721 ccctgcttcg cagagccgcc cgcggagtcc ccttccacaa ggtacagttc ggaaagcgcc
    24781 ggatcgcgtt cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct
    24841 ttacggcggg tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata
    24901 attttgccaa ccacgatttt cgcgtcagtt gggttttcca gcaggtattc cgccaacagt
    24961 tcgttcatct gctgttcaac cgccgatttc acctcagaag aaaccagttt gtctttggtc
    25021 tgggaggaga atttcgggtc cggcactttt acggaaacga ccgcaatcag gccttcacgc
    25081 gcatcgtcac cggtggcgct gactttggct tttttgctgt agccttcttt gtccatgtag
    25141 gcattcaggg tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc
    25201 tgcggaatgt tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcagc
    25261 gccacttcaa cgccgatacc gtctttttcg gtggagaagt agaagatatt tgggtggatc
    25321 ggcgttttgt tcttgttcag atattcaacg aacgctttga tgccgccttc atagtggaag
    25381 tggtcttctt tgccgtcacg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac
    25441 gacaactcac gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag
    25501 gtttcgaggc tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc
    25561 gccagcggtg cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc
    25621 tgaataacca gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc
    25681 agaccgccgg acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc
    25741 atgatcactt ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc
    25801 ccgtcatcct gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag
    25861 tgacccgcga gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga
    25921 ccggtgccgt catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc
    25981 cctttcagga ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca
    26041 tttatacttg ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc
    26101 gtccgacatg tctataacgt gttcagcact gatcgcgctg acaaagacct gcgattgcgt
    26161 agcttttaag cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc
    26221 atctatcagg tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag
    26281 acgtaaggcg cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc
    26341 accgtcggcg cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg
    26401 gcgatcgcgt tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc
    26461 gcgctggaaa gagaaagtca gagaaaactc agggagaaat tgcttacagg tatcagccat
    26521 atcggccgcg ataccggcgc tatactccgc gcgccaggta ctgatttgct ccgccagcgg
    26581 gatcagttct ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc
    26641 attgcgctgc ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt
    26701 gtgaaagcat ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt
    26761 aaacccttct ggcgttatca gctgcattgg catcaggtgc gccagttccg cgactttatg
    26821 cccgtcagta ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat
    26881 cgccgtctcg cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcgtg
    26941 gcgaatgacg cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat
    27001 ggcttccagc acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg
    27061 agataaagcg agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag
    27121 ggacattaca gtctcattgg catgacaaca taagccgcgc tctggctggc tgcatcttca
    27181 atctgcacgc tggaaaccga atcggtcagc atcatgcgga cgttttcgca tttcagcgcg
    27241 ttcagaacat ccagcacata gctgacgttg aagccgattt ccatctccgc accgctatag
    27301 gtaacgtcga ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc
    27361 tggttttcgc tgacatacag gcgcacaccg cggaatttct cgttagagag aattgccgcg
    27421 cgagcaaacg cctgcttgag gagatcgcag cctgcttcca gatgtttgtc cggattcttc
    27481 ggcagaacgc ggcggtaatc cgggaagcga ccatccacca gtttagaggt gaagataaag
    27541 tcgccaacgt gggcgcgaat attgttgctg ccaatctgca cacgcagcgg attatcgccg
    27601 ccgtcgagca tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt
    27661 ggcaaagatt gaccaattgg cattgaacag accgccagac ggtggccgtc ggtcgctacg
    27721 gtgcgcagtt cttcaccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc
    27781 tgatgcgcca tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg
    27841 gtaaattcga cttcactctg ccagtcatcg aggttcggga aatccgccgc tggcagggta
    27901 gacagcgaaa aacggctacg cccggagcgc accagcatcc gctcaccttc cagctgcacg
    27961 gcaatttccg cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg
    28021 gtcgtcgctc ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg
    28081 agatcagtac cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga
    28141 atcggtagcg taggacgacc acctaacgga ccgctcacct gttgtagcgg ttttaataaa
    28201 tgctcacgtt ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga
    28261 aaaatcttct ttgatatcgt ggctctcttc acgcagctgc tcgatcttcc ggcaggcatg
    28321 aagcaccgta gtgtggtcac gaccaccaaa cgcatcgcca atctctggca gactgtggtt
    28381 agtcagctct ttcgccagcg ccatcgccat ctgacgcgga cgagccaccg agcgggatcg
    28441 acgcttggaa aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat
    28501 attgtcgatg gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac
    28561 gaagtcgata gtgatcgccc gtccggtaaa gttggcgttg gcaatgacgc ggttcagcgc
    28621 gccttccagc tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc
    28681 tggcaaacga atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttctaa
    28741 ctctggcggt tcgatcgcca cagtcagccc ccagccgaag cgggatttca aacgatcctc
    28801 aacgccgttg atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc
    28861 cagcagggcg ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa
    28921 ctgaatatcg tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat
    28981 cgcgttgttt tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac
    29041 cactttggca ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt
    29101 tttacccaga cccgtgccgc cataaaggaa caacgggtta taggcaccgc cagggttatc
    29161 tgccacctgg cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc
    29221 aaacgtgtgt ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt
    29281 atcccaacct gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc
    29341 aggggccgcg acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg atttggtgcc
    29401 gacttcaaaa cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat
    29461 attattaagg tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc
    29521 cagcgtgtta tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc
    29581 tggtaactca tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga
    29641 ctccactcga acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac
    29701 gtcgaaaaga ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat
    29761 caagatcctg caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc
    29821 tgtttctcac agattttccc gatttatcca caggatgatc cagaactcgc taagtgtaaa
    29881 cgatcctgcc gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag
    29941 acaaaaattg gcttaatcga tctaataaag atccaggacg atccttgcgc tttacccatc
    30001 agcccgtata atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggccgtacg
    30061 ttttccctgc gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga
    30121 gtgtacaatt attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc
    30181 atttttcggc ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc
    30241 atgaaacgca cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct
    30301 cgtatggcta ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct
    30361 cgtctgaccg tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag
    30421 ggagttacgc ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc
    30481 tggcacgccg caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg
    30541 tcttacagtc gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct
    30601 gacgcgtgaa agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt
    30661 ggcgaaaaaa ggggttgcca acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt
    30721 atggcgccgc cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa
    30781 cgcctgatta gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac
    30841 ggaattgagg cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc
    30901 gtattaaaat gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt
    30961 aataccagag aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt
    31021 tcgtgtcttt catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc
    31081 aacagaccac gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg
    31141 ccagtggcca ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca
    31201 cccgtggtgg tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta
    31261 cccagccgtt ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc
    31321 tgaccggtcg tgatggccca gataacccgg ctaacggccc gcgtccgctg tataacgttg
    31381 aaaaagacgc ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata
    31441 ccgacgcggc aggcaacacg tttaccaaaa cgtttgtcct gaaacggggt gattacgctg
    31501 tcaacgtcaa ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg
    31561 gtcagttgaa gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg
    31621 cactgcacac cttccgtggc gcggcgtact ccacgcctga cgagaagtac gagaaataca
    31681 agttcgatac cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg
    31741 caatgctgca acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact
    31801 tctatactgc taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac
    31861 tggttcagcc tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc
    31921 aggacaaaat ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt
    31981 ggttcatctc tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact
    32041 ggggcttctc cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca
    32101 aagcgcagta cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc
    32161 gtgagcgtct gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag
    32221 ctgagaaggt taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc
    32281 tggcgttgta ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt
    32341 ggatccacga cctgtctgca caggacccgt actacatcct gccgatcctg atgggcgtaa
    32401 cgatgttctt cattcagaag atgtcgccga ccactgtgac cgacccgatg cagcagaaga
    32461 tcatgacctt tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg
    32521 tgctgtacta tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg
    32581 gtctggaaaa acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt
    32641 cgctaaaata agggcggtca gttgaccgcc ttttttcttt tcgtagggcg gataagcacc
    32701 gcgtatccgc cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc
    32761 aggccacgcc tccgggacgt ggcggcgttg gcatcctgcg catctccggc ttcaaagccc
    32821 gtgaagttgc cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc
    32881 cgtttaaaga cgccgatggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc
    32941 cgaactcgtt caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgattc
    33001 tcgacctgct gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg
    33061 agttttccga acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg
    33121 ccgatcttat cgatgccagt tcggaacagg cagcccgttc ggcacttaac tcgctgcaag
    33181 gcgcattctc cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg
    33241 tcgaagcggc aattgatttc cccgatgaag agatcgattt cctctccgac ggaaaaattg
    33301 aagcccagct taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg
    33361 gtagtttgtt gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaat gccggtaaat
    33421 cgagcctgtt aaacgcgctg gctggccgtg aagcagcaat cgtaaccgat atcgccggaa
    33481 ccacgcgtga cgtgttgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg
    33541 acaccgccgg gctacgtgaa gccagcgatg aagtggaacg tattggtatc gagcgcgcgt
    33601 ggcaagaaat tgaacaagcc gaccgcgtgc tgtttatggt cgatggcacc acgacagacg
    33661 ccgttgatcc ggcggagatc tggccggaat ttatcgcccg tctgcccgcg aaactgccga
    33721 tcaccgtggt acgcaataaa gccgatatca ctggcgaaac gctgggaatg agtgaagtga
    33781 acggtcatgc gttgattcgt ctctcggcaa agactggcga aggcgtggac gtgctgcgta
    33841 accatctcaa acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc
    33901 gtcgccacct tcaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac
    33961 tgttgggtgc ctgggcgggc gaactgctgg cggaagagtt gcgcctggca cagcagaact
    34021 taagcgaaat caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct
    34081 tctgtattgg taagtaagtc gtctaaattg atataaaggg cactgttccg ttctggggga
    34141 aactaactat cagaacggaa cagcgcttgt ttttttatga gcatcctgca ccaaaactgc
    34201 ctttactact ggctggagcg actttcaaca tcttgcagtc ctgtctttac agagaacgat
    34261 attgctttag ccgattaagc tcctcttgcg gaaagttaaa ttcgtctatt gctttccaga
    34321 atagttcttt aaatacagga ttgccatttg ccttgcttat tacatatttg ccttcattat
    34381 tcttcgcttc cagttgcatc atcacatctt taagataatt ttcattttca ccaaccacaa
    34441 ttttgaatat tttcagccag agacccatat atatcatccg ctttctctac cgcaagagag
    34501 aatgacttcc catcaaagtc acgggtaaac tgctccaaaa tctcatcgaa agtgaacccg
    34561 tgttgcaacg ccaggcgaat cgccgcattc agatatacat caatgacctg cttactcatt
    34621 gtcttatagc tggcaacata caaagagcaa actgcattat ttctgctcag cgacaggagt
    34681 tctctcacta catctttatc attttcataa tggctggcta tcacttcagc tgtctccatc
    34741 caggcagcaa caatcttcgt atgccctttc actatcgcag aatataagcc tgatgccttc
    34801 gtccatttgt ctgatgctcc cgccactttt tctcttgctg ccatcagttt caatatatct
    34861 tctttattta attcattttt aaac
//
LOCUS       DABSYT020000041        34722 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.041, whole genome shotgun sequence.
ACCESSION   DABSYT020000041 DABSYT020000000
VERSION     DABSYT020000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.041"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            29..730
                     /gene="elfD"
                     /locus_tag="ID725_RS12060"
                     /old_locus_tag="pgaptmp_002412"
     CDS             29..730
                     /gene="elfD"
                     /locus_tag="ID725_RS12060"
                     /old_locus_tag="pgaptmp_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGV7850998.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            755..3354
                     /gene="elfC"
                     /locus_tag="ID725_RS12065"
                     /old_locus_tag="pgaptmp_002413"
                     /pseudo
     CDS             755..3354
                     /gene="elfC"
                     /locus_tag="ID725_RS12065"
                     /old_locus_tag="pgaptmp_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3345..4415
                     /gene="elfG"
                     /locus_tag="ID725_RS12070"
                     /old_locus_tag="pgaptmp_002414"
                     /pseudo
     CDS             3345..4415
                     /gene="elfG"
                     /locus_tag="ID725_RS12070"
                     /old_locus_tag="pgaptmp_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4443..4970
                     /gene="ycbU"
                     /locus_tag="ID725_RS12075"
                     /old_locus_tag="pgaptmp_002415"
     CDS             4443..4970
                     /gene="ycbU"
                     /locus_tag="ID725_RS12075"
                     /old_locus_tag="pgaptmp_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7850999.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            4978..5493
                     /gene="ycbV"
                     /locus_tag="ID725_RS12080"
                     /old_locus_tag="pgaptmp_002416"
     CDS             4978..5493
                     /gene="ycbV"
                     /locus_tag="ID725_RS12080"
                     /old_locus_tag="pgaptmp_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7851000.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5459..6195
                     /gene="ycbF"
                     /locus_tag="ID725_RS12085"
                     /old_locus_tag="pgaptmp_002417"
                     /pseudo
     CDS             5459..6195
                     /gene="ycbF"
                     /locus_tag="ID725_RS12085"
                     /old_locus_tag="pgaptmp_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6306..7316
                     /gene="pyrD"
                     /locus_tag="ID725_RS12090"
                     /old_locus_tag="pgaptmp_002418"
     CDS             6306..7316
                     /gene="pyrD"
                     /locus_tag="ID725_RS12090"
                     /old_locus_tag="pgaptmp_002418"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGV7851001.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7490..8032
                     /gene="zapC"
                     /locus_tag="ID725_RS12095"
                     /old_locus_tag="pgaptmp_002419"
     CDS             7490..8032
                     /gene="zapC"
                     /locus_tag="ID725_RS12095"
                     /old_locus_tag="pgaptmp_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGV7851002.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8029..9138)
                     /gene="ycbX"
                     /locus_tag="ID725_RS12100"
                     /old_locus_tag="pgaptmp_002420"
     CDS             complement(8029..9138)
                     /gene="ycbX"
                     /locus_tag="ID725_RS12100"
                     /old_locus_tag="pgaptmp_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGV7851003.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9382..11490
                     /gene="rlmKL"
                     /locus_tag="ID725_RS12105"
                     /old_locus_tag="pgaptmp_002421"
     CDS             9382..11490
                     /gene="rlmKL"
                     /locus_tag="ID725_RS12105"
                     /old_locus_tag="pgaptmp_002421"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGV7851004.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11502..13409
                     /gene="uup"
                     /locus_tag="ID725_RS12110"
                     /old_locus_tag="pgaptmp_002422"
     CDS             11502..13409
                     /gene="uup"
                     /locus_tag="ID725_RS12110"
                     /old_locus_tag="pgaptmp_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGV7851005.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13539..14792
                     /gene="pqiA"
                     /locus_tag="ID725_RS12115"
                     /old_locus_tag="pgaptmp_002423"
     CDS             13539..14792
                     /gene="pqiA"
                     /locus_tag="ID725_RS12115"
                     /old_locus_tag="pgaptmp_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGV7851006.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14797..16437
                     /gene="pqiB"
                     /locus_tag="ID725_RS12120"
                     /old_locus_tag="pgaptmp_002424"
     CDS             14797..16437
                     /gene="pqiB"
                     /locus_tag="ID725_RS12120"
                     /old_locus_tag="pgaptmp_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGV7851007.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16434..16997
                     /gene="pqiC"
                     /locus_tag="ID725_RS12125"
                     /old_locus_tag="pgaptmp_002425"
     CDS             16434..16997
                     /gene="pqiC"
                     /locus_tag="ID725_RS12125"
                     /old_locus_tag="pgaptmp_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGV7851008.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17253..17420
                     /gene="rmf"
                     /locus_tag="ID725_RS12130"
                     /old_locus_tag="pgaptmp_002426"
     CDS             17253..17420
                     /gene="rmf"
                     /locus_tag="ID725_RS12130"
                     /old_locus_tag="pgaptmp_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGV7851009.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17490..18008)
                     /gene="fabA"
                     /locus_tag="ID725_RS12135"
                     /old_locus_tag="pgaptmp_002427"
     CDS             complement(17490..18008)
                     /gene="fabA"
                     /locus_tag="ID725_RS12135"
                     /old_locus_tag="pgaptmp_002427"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGV7851010.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18077..19837)
                     /gene="ycbZ"
                     /locus_tag="ID725_RS12140"
                     /old_locus_tag="pgaptmp_002428"
     CDS             complement(18077..19837)
                     /gene="ycbZ"
                     /locus_tag="ID725_RS12140"
                     /old_locus_tag="pgaptmp_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGV7851011.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20023..20475
                     /gene="matP"
                     /locus_tag="ID725_RS12145"
                     /old_locus_tag="pgaptmp_002429"
     CDS             20023..20475
                     /gene="matP"
                     /locus_tag="ID725_RS12145"
                     /old_locus_tag="pgaptmp_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGV7851012.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20550..21590)
                     /gene="ompA"
                     /locus_tag="ID725_RS12150"
                     /old_locus_tag="pgaptmp_002430"
     CDS             complement(20550..21590)
                     /gene="ompA"
                     /locus_tag="ID725_RS12150"
                     /old_locus_tag="pgaptmp_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGV7851013.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21947..22456)
                     /gene="sulA"
                     /locus_tag="ID725_RS12155"
                     /old_locus_tag="pgaptmp_002431"
     CDS             complement(21947..22456)
                     /gene="sulA"
                     /locus_tag="ID725_RS12155"
                     /old_locus_tag="pgaptmp_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGV7851014.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22675..23304
                     /gene="sxy"
                     /locus_tag="ID725_RS12160"
                     /old_locus_tag="pgaptmp_002432"
     CDS             22675..23304
                     /gene="sxy"
                     /locus_tag="ID725_RS12160"
                     /old_locus_tag="pgaptmp_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGV7851015.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23267..25429)
                     /gene="yccS"
                     /locus_tag="ID725_RS12165"
                     /old_locus_tag="pgaptmp_002433"
     CDS             complement(23267..25429)
                     /gene="yccS"
                     /locus_tag="ID725_RS12165"
                     /old_locus_tag="pgaptmp_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGV7851016.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25439..25885)
                     /gene="yccF"
                     /locus_tag="ID725_RS12170"
                     /old_locus_tag="pgaptmp_002434"
     CDS             complement(25439..25885)
                     /gene="yccF"
                     /locus_tag="ID725_RS12170"
                     /old_locus_tag="pgaptmp_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGV7851017.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26008..28062
                     /gene="helD"
                     /locus_tag="ID725_RS12175"
                     /old_locus_tag="pgaptmp_002435"
     CDS             26008..28062
                     /gene="helD"
                     /locus_tag="ID725_RS12175"
                     /old_locus_tag="pgaptmp_002435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGV7851018.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28094..28552)
                     /gene="mgsA"
                     /locus_tag="ID725_RS12180"
                     /old_locus_tag="pgaptmp_002436"
     CDS             complement(28094..28552)
                     /gene="mgsA"
                     /locus_tag="ID725_RS12180"
                     /old_locus_tag="pgaptmp_002436"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGV7851019.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28648..29310)
                     /gene="yccT"
                     /locus_tag="ID725_RS12185"
                     /old_locus_tag="pgaptmp_002437"
     CDS             complement(28648..29310)
                     /gene="yccT"
                     /locus_tag="ID725_RS12185"
                     /old_locus_tag="pgaptmp_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGV7851020.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29483..29896
                     /gene="yccU"
                     /locus_tag="ID725_RS12190"
                     /old_locus_tag="pgaptmp_002438"
     CDS             29483..29896
                     /gene="yccU"
                     /locus_tag="ID725_RS12190"
                     /old_locus_tag="pgaptmp_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGV7851021.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29941..30258)
                     /gene="hspQ"
                     /locus_tag="ID725_RS12195"
                     /old_locus_tag="pgaptmp_002439"
     CDS             complement(29941..30258)
                     /gene="hspQ"
                     /locus_tag="ID725_RS12195"
                     /old_locus_tag="pgaptmp_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGV7851022.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30316..31506)
                     /gene="rlmI"
                     /locus_tag="ID725_RS12200"
                     /old_locus_tag="pgaptmp_002440"
     CDS             complement(30316..31506)
                     /gene="rlmI"
                     /locus_tag="ID725_RS12200"
                     /old_locus_tag="pgaptmp_002440"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGV7851023.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31601..31879
                     /gene="yccX"
                     /locus_tag="ID725_RS12205"
                     /old_locus_tag="pgaptmp_002441"
     CDS             31601..31879
                     /gene="yccX"
                     /locus_tag="ID725_RS12205"
                     /old_locus_tag="pgaptmp_002441"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGV7851024.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31876..32205)
                     /gene="tusE"
                     /locus_tag="ID725_RS12210"
                     /old_locus_tag="pgaptmp_002442"
     CDS             complement(31876..32205)
                     /gene="tusE"
                     /locus_tag="ID725_RS12210"
                     /old_locus_tag="pgaptmp_002442"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGV7851025.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32296..32955)
                     /gene="yccA"
                     /locus_tag="ID725_RS12215"
                     /old_locus_tag="pgaptmp_002443"
     CDS             complement(32296..32955)
                     /gene="yccA"
                     /locus_tag="ID725_RS12215"
                     /old_locus_tag="pgaptmp_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGV7851026.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33363..34369)
                     /locus_tag="ID725_RS12220"
                     /old_locus_tag="pgaptmp_002444"
                     /pseudo
     CDS             complement(33363..34369)
                     /locus_tag="ID725_RS12220"
                     /old_locus_tag="pgaptmp_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(34347..34589)
                     /locus_tag="ID725_RS12225"
                     /old_locus_tag="pgaptmp_002445"
     CDS             complement(34347..34589)
                     /locus_tag="ID725_RS12225"
                     /old_locus_tag="pgaptmp_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGV7851027.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         8918 a         8293 c         9015 g         8496 t
ORIGIN      
        1 tattcatatt ttttaagagt gactatttat gaaaacttgc ataacaaagg gaattgtgac
       61 cgtaagttta acggcaatat tactcagctg ttcgtccaca tgggcggcag gtaaaggcgg
      121 ggttggactt gcagcaacac gtcttgttta ttcggaaggt gaagagcaaa tttcactggg
      181 tgtacgtaac accagtccgg atgttcctta tctgattcag tcatgggtga tgaccccaga
      241 taataaaaaa tcagcggact ttattattac accaccgcta tttgtgctga atccggcaaa
      301 cgagaatctg ttacgcatta tgtacattgg cgcgccgttg gcgaaagaca gagaaactct
      361 tttcttcact agcgtacggg cagtcccttc aacaacgaag cgggaagaag gcaataccct
      421 gaagattgcc acacaaagcg tcatcaaact tttctggcga ccaaaaggtt tagcgtatcc
      481 cttaggcgag gctccggcga aactgcgttg cacttcgtca gctgacatgg ttacggtcag
      541 taacccaaca ccttatttca ttaccctgac agacctgaaa ataggtggaa agttagttaa
      601 aaatcaaatg atttccccct ttgataaata ccaattctct ctgccaaagg gtgccaaaaa
      661 tagcagcgta acgtatcgaa ccatcaatga ctacggggcg gaaacgccgc aactcaactg
      721 taaatcgtaa ttcgtcttca gttaagagag cgagatgtat agaactcacc gacaacacag
      781 cctgttaagc tctggtggag tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc
      841 agcgttcaat gcacaagctg aaacctggtt cgatccagcc tttttcaaag atgatccctc
      901 aatggtggcc gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg
      961 tgtcgatatt gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat
     1021 aacgccagag aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggcgt
     1081 gaatactgat gcatttccgg cttttaaaca actggacaaa caagcgtgtg cgccattggc
     1141 ggagattatt ccggatgcca gcgtaacttt taatgtgaat aaactccgtc tggaaatttc
     1201 agtaccgcaa attgccatca aaagtaatgc tcgtggttat gtccccctga acgttgggat
     1261 gaagggatca acgcgctatt actgggatat tcatttagcg gggctaacag tattcatagc
     1321 agcgcagaca gtgattctgg cgacagttat tttctgaatt taaacagtgg cgttaattta
     1381 ggcccatgga gattgcgcaa caattcaaca tggagtcgta gtagtggcca aaccgcagaa
     1441 tggaagaatc tcagcagcta tttgcaacgg gcggttattc ctctgaaagg cgaactgacc
     1501 gtaggtgatg attatactgc aggcgatttt ttcgatagtg tcagctttcg tggtgtgcag
     1561 ctggcgtcag atgacaacat gctgccagac agcctgaaag ggtttgcgcc tgtggtgcgt
     1621 ggtatcgcca aaagcaatgc ccagataacg attaagcaaa atggttacac catttaccaa
     1681 acttatgtat cgcctggtgc ttttgaaatt agtgatatct attccacgtc gtcgagcggt
     1741 gatttgttag ttgaaatcaa agaagcggac ggcagcgtca atagctacag cgtacccttt
     1801 tccagcgtgc cattactcca gcgtcagggg cgaatcaaat acgcggtgac actggcgaaa
     1861 tacagaacca atagtaatga acagcaggag agcaaatttg cccaggccac gttgcagtgg
     1921 ggcggaccgt ggggaacgac ctggtatggt ggtggacaat atgctgaata ttaccgtgcc
     1981 gccatgtttg gtctgggatt taacctcggc gatttcggag caatttcgtt cgatgtgacc
     2041 caggcgaaga gtacgctggc agaccaaagc gaacataaag gtcagtcata tcgttttctg
     2101 tatgccaaaa cgctcaacca attgggtact aactttcaat tgatgggcta tcgctattcg
     2161 acgtcgggtt tctacaccct ttccgacacc atgtataaac atatggatgg ctacgaattt
     2221 aatgacggtg atgatgaaga tacgccgatg tggtcgcgtt attacaattt gttttacacc
     2281 aaacgtggca aactgcaggt caatatctcc cagcaattaa gcgagtacgg ttcgttttat
     2341 ttaagtggta gccagcaaac ttactggcat accgatcaac aggatcggct attacagttt
     2401 ggctataaca cgcaaattaa agatctctcg ctggggatct cctggaacta cagtaagtcc
     2461 cgtggccaac ctgatgctga tcaggtgttt gcacttaatt tttccctgcc actcaatctg
     2521 ttgctctcca gaagtaatga tagctatacc agcaaaaaaa attacgcctg gatgacctct
     2581 aacaccagta tcgataacga agggcacact acacaaaacc tgggtttaac ggagacacta
     2641 ctcgatgacg gtaacctgag ctacagcgtg caacagggat ataacagcga ggggaaaacg
     2701 gctaatggca gcgccagcat ggactacaaa ggggtgtttg cagatgcccg agtgggctac
     2761 aactacagcg ataacggcag tcaacaacaa ctgaactacg ctctttcagg cagtttagtt
     2821 gcccattcac agggcattac cctggggcaa tcactggggg aaactaacgt tctgattgca
     2881 gcaccaggcg cagagaatac tcgtgtggcg aacagcaccg ggctgaaaac tgactggcgc
     2941 ggatataccg ttgttcctta tgccacttct tatcgtgaaa atcgaatcgc acttgatgcg
     3001 gcgtcgttaa aacgtaacgt ggatcttgaa aatgcagtag tcaacgtggt tcccaccaaa
     3061 ggggcgttgg ttctggcgga gttcaatgcc catgcgggag caagggtatt aatgaaaaca
     3121 tcaaagcagg gtatatcgct gcgttttggc gcgatagcga cgctggacgg cgtacagact
     3181 aatagcggca taattgatga tgatggctcg ctctatatgg cgggtttacc ggcgaaggga
     3241 acaataaccg tgcgctgggg cgaagctccc gatcaaattt gtcatatcag ttacgagctt
     3301 accgaacaac aaattaactc tgcgattacg cgaatggatg ccatatgcag ataatctttg
     3361 gagaaaaatg cgtgtcatta ctacgactat tttttgccgc cgtcttaatg ctatggtgcg
     3421 ctcaaaccgc tgcttatagc gggcagtgtc ataccactca ggggaatccg tatattggcg
     3481 tcaattttgg cgttaaaacc ctggaggaag aagaaaatac gactggggta gtaaaagaca
     3541 aattttatca gtggaacgaa tcgaatgatt attatgtttc ctgtgattgc gataaagaca
     3601 atgtcagaag tggccgatgg gcattcgccg cggattcacc gttagtctat ttaggcgaca
     3661 actggtacaa aattaatgac tatcttgccg ccaaagtttt attgcaggtt aaaggcagtt
     3721 ctcctacagc ggttcctttc gaaaacgtgg ggactggggc agatacccgg tggcatattt
     3781 gtgaccccgg cggtcaacgt ttaggcggcc agggagctag cggtaatagc ggtagctttt
     3841 ccctgaaaat attgcagccg ttcgttggtt cggtcgtcat tcctcctatg gcgctggcgc
     3901 gattatttga atgctacaac atacccgcag gtgattcctg cacgactaca ggcacaccgg
     3961 ttttagtgta ttacctgtct ggtactatca attcacttgg ctcatgttcc gtcaatgccg
     4021 gagaaacaat cgaggtcgat ctgggcgacg tatttgcggc taactttcgt gttgtagggc
     4081 ataagcctct tggggccaga acggcagaac ttgcaattcc agtcaggtgt aacacgggaa
     4141 acgcggggtt agttaacgtc aacctgagtc tgacggcaac cacagacccc agctatcccc
     4201 aggcgattaa gacgtcacgt cctggcgtgg gcgtggtggt gaccgatagc cagaacaaca
     4261 ttatttcccc tgctggtgga acattaccgc tctctattcc tgatgatgca gacagtatcg
     4321 cgtgaatgaa tgtctatcca gtcagcacga caggtgtacc accagaaacc gggcgatttg
     4381 aagccacggc aacggtgaga ataaattttg attaacgcgt gaacgtatga agaaaaaaaa
     4441 cgatatttca gtgcgttatc ttgttcttta gcattcttaa catccatgtc gggatggctg
     4501 ggcctgaaca agttagtatg catatttatg ggaatgtggt cgatcagggc tgtgatgtcg
     4561 ccaccaaaag tgcattacaa aatattcata ttggtgattt taatatcagt gattttcagg
     4621 ccgcgaatac cgtaagcact gctgctgatt tgaatatcga tatcaccggt tgtgccgctg
     4681 gtattactgg cgcggacgtc ctttttagcg gcgaggctga cacccttgcg ccgacactgc
     4741 tcaaactaac tgacacaggc ggaagcggtg gtatggcaac ggggattgcc gtgcaaattc
     4801 ttgatgcgca aagtcagcaa gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa
     4861 aagccgggga taacacactc aaatatcaac ttcgttataa gtccacaaag gcgggagcaa
     4921 cgggcggtaa tgcgacggcg gttctctatt ttgatctggt ttaccagtga aggagtgatg
     4981 ttgaaacgga taatctggat tctgttctta ctgggattaa cgggggggtg tgagctattt
     5041 gcccatgatg gcacggtcaa cattagcgga tcgtttcgcc gtaatacatg cgtgctggca
     5101 caggatagca agcaaatcaa tgtgcagtta ggcgatgtct cactgacacg tttttctcat
     5161 gggaattatg gcccggagaa atctttcatc attaatttgc aggattgcgg aacagatgtc
     5221 agcacggttg acgtcacctt ttcaggaacc cccgacggag tgcagagcga aatgttgtcc
     5281 attgaaagtg gtacggatgc agccagcggg ctggcaattg cgattctgga tgatgcgaag
     5341 atattgattc cgcttaacca ggcgagtaaa gactacagcc tacatagcgg taaggtgcca
     5401 ttaacttttt atgcgcaatt gcgacctgtc aatagcgatg tgcagtcggg taaagtgaat
     5461 gccagcgcga catttgtact tcattatgac taatacgtgg aacagattgg cgctcttgat
     5521 tttcgccgtt ttatcgttgc tggtggcggg tgaattgcag gcaggagtgg tggtcggcgg
     5581 aacgcgattt atctttccgg cagacagaga atcgatatct attttactga ctaataccag
     5641 tcaggaatcc tggcttatta acagtaaaat caaccgccca acgcgttggg ccgggggtga
     5701 agcgtcgaca gtgccagcac cattactggc cgctccgcca cttattctcc tgaagcccgg
     5761 tacgacaggc acgttgcgct tgctgagaac ggaaagcgac atcttgcctg tggatcgcga
     5821 aacgctattt gagttaagca ttgccagcgt gccatccggc aaagttgaaa atcagagcgt
     5881 aaaagtggcg atgcgctcgg tatttaaact gttctggcga cccgaaggtt gccgggcgac
     5941 ccgctggaag cttatcaaca attacgctgg acacggaagt cgcagggtgt acaactcact
     6001 aacccaacgc cttattacat taacctgatt caggtgagcg taaatggtaa agcgttaagt
     6061 aatgtgggag ttgtgccgcc taaaagccag cgtcagacaa gctggtgtca ggcaatagca
     6121 ccgtgtcatg ttgcctggcg ggcgataaat gattacggcg ggttgagtgc aaagaaggag
     6181 caaaatctgc cctgaaacag gttcggaaaa cgtttgcgtt ttttttgccg caggtcaatt
     6241 cccttttggt ccgaactcgc acataatacg cccccggttt gcacaccggg aatccaggag
     6301 agttcatgta ctaccccttc gttcgtaaag cccttttcca gctcgatcca gagcgcgctc
     6361 atgagtttac ttttcagcaa ttacgccgca ttaccggaac accgtttgaa gcactggtgc
     6421 ggcagaaagt gcctgcaaaa cctgttaact gcatgggcct gacgtttaaa aatccgcttg
     6481 gtctggcagc cggtcttgac aaagacgggg agtgcattga tgcgttaggc gcgatgggat
     6541 ttggatcgat agagatcggt accgtcacgc cacgtccaca gccaggtaat gacaagccgc
     6601 gtctctttcg tctggtagat gccgaaggtt tgatcaaccg tatgggcttt aataatcttg
     6661 gcgttgataa cctcgtagag aacgtaaaaa aagcccatta tgacggcgtc ctgggtatta
     6721 acatcggcaa aaataaagat acgccagtag agcagggcaa agatgactat ctgatttgta
     6781 tggaaaaaat ctatgcctat gcgggatata tcgccatcaa tatttcatcg ccaaataccc
     6841 caggattacg cacactgcaa tatggtgaag cgctggatga tctcttaact gcgattaaaa
     6901 ataaacaaaa tgatttgcaa gcgatgcacc ataaatatgt gccgatcgca gtgaagatcg
     6961 cgccggatct ttctgaagaa gaattgatcc aggttgccga tagtttagtt cgccataata
     7021 ttgatggcgt tattgcaacc aataccacac tcgatcgttc tctggttcag ggaatgaaaa
     7081 attgcgatca aaccggtggc ttaagtggtc gtccgcttca gttaaaaagc accgaaatta
     7141 ttcgccgctt gtcactggaa ttaaacggtc gcttaccgat catcggtgtt ggcggcatcg
     7201 actcggttat cgctgcgcgt gaaaagattg ctgcgggtgc ctcactggtg caaatttatt
     7261 ctggttttat ttttaaaggt ccgccgctga ttaaagaaat cgttacccat atctaattat
     7321 ttcttcgaat tcctctttat aagataatca gggctttatt ttcagccctg gttgttttat
     7381 attcattgct gttgcttatt tagacatttt gtacttttat tgttgaggtt attaagcgaa
     7441 gcgacaatgg attgtgttgc tgcggtttta tagggtaggg gagaggcaga tgcgaattaa
     7501 accagacgat aactggcgtt ggtattacga tgaagagcac gatcgtatga tgctcgattt
     7561 agccaatggt atgctatttc gctcacgttt tgcgcgcaaa atgttgactc cagatgcctt
     7621 ttctcccgca ggcttttgcg ttgacgacgc cgcgctctat ttctcttttg aagaaaagtg
     7681 ccgcgatttt aatttatcta aagaacaaaa agccgaactg gtgctgaatg cactggtagc
     7741 aattcgctat ctcaaaccgc aaatgccgaa aagctggcat tttgtttccc atggtgaaat
     7801 gtgggtgccc atgccgggcg atgcggcctg cgtttggttg agcgacacta acgagcaagt
     7861 gaatttactg gtcgttgaat ctggcgagaa tgccgcgcta tgcctgctgg cacaaccctg
     7921 cgttgttatt gcgggtcgcg cgatgcagtt gggcgacgcc attaaaatca tgaacgacag
     7981 gctgaaaccg caggttaatg ttgacagctt cagcctcgaa caggcagtct aacgcgccaa
     8041 cttaagtgca gtcttcggta cacagctaca gcaaagaatg gtgccatcat cgcccattgc
     8101 tgattttttc agcggcgtga cttcgccttc taaaagctga acacggcaac ttccacaaat
     8161 gcccgcgcgg caagaataag ggatacgaat tccctgattt tctaattgct ccagcaacac
     8221 ctgttggtta tttccacgaa atgcctgtcc ctgccagtca atatctacat tggcgtccgg
     8281 ttgttgcgtg atgttggcag tatcatcagc ggcagctgcg ccgtaaattt ttgccggagc
     8341 cgttgtcaga atttccacct catcgccaac gcgaatcacg ccgctattac gggcaattaa
     8401 attctgacca aaatcgacat cgccgttatc ctgggcagtg cggaaagatt gcaatgtttt
     8461 taatggttcg cctgccggat gtttttgccc tttttctggg ctgacggtgg tgaaaataca
     8521 gcggctacaa ggtttaacca catcaaacac cacatcacca atgcgaatca ctttccaact
     8581 atcttcttcc caggctgacg cgccggaaac caccagattg gggcggaatt gctccatttt
     8641 tacactggcc ggacaacgtt gttggagatc acgtaacgag gcttcgttag caagaaggta
     8701 aggatagcca tcagcaaatg acaggggtac agtgttgtgg cgtttcacgc gccgggtcat
     8761 ttgtggcccc acccagcgta attgcacttc gcgggagaaa aatccactta accatttgtt
     8821 gatcgcttct ggcgcaattc gcgcggtaaa atgtgtgccc caaacttcgg ttggtgcgtc
     8881 ttgtgtggcg aaatcagcaa aacgaacata tgcgctactg ctatctggtg cggtgagatg
     8941 caagccatca tgcacgggtg aaggagtaaa tcgtaccatc tggggaaact ggcgagcggt
     9001 gataaacgta ccgtcaggtt ccgtgatcat aaagatgcga tcgaaggcca gaccactgac
     9061 atctgccaga gcatgtgtaa gaccaatgcc gcgcatcgat ttaacaggat gaataaaaag
     9121 ccgggttaat gtcgccacgg tgcggtcctc aaatgaaaat aagccctcaa ctttatgaca
     9181 tgacgcgctt attagctata atgcgcaaca attttcttag cgcctgtccc attaggctat
     9241 tttatttgtc agtttggccc tgggttgtgc tcgaaatcct cacgtacttg tgtacgctcc
     9301 ggtttctccg cgctgtccat gtccagactg actgcaacaa ttacgcctac tgcggtaggc
     9361 tcttagagta aaagtgacga tatgaattct ctgtttgcca gtacggcccg tgggctggaa
     9421 gagctgttaa aaactgaact ggaaaacctg ggggccgttg aatgccaggt ggttcagggt
     9481 ggggtccatt tcaagggcga cacacggctt gtttaccaga gcctgatgtg gagccgcctg
     9541 gcctcgcgta ttatgttgcc gctgggcgag tgtaaggttt acagcgattt agacctctat
     9601 ctcggtgttc aggcgatcaa ctggacagag atgtttaatc ctggcgcgac cttcgctgtc
     9661 cacttcagtg gtttgaatga cactatccgc aacagtcagt acggcgcgat gaaagtgaaa
     9721 gacgcgatcg tcgatgcttt cacgcggaaa aatctgccgc gtccaaatgt tgatcgcgat
     9781 gcgccggata tccgcgttaa cgtctggctg cataaagaaa ccgccagtat cgcccttgat
     9841 ctcagtggtg atggtttaca tctgcgtggc tatcgcgatc gtgctggtat tgcgccgatc
     9901 aaagaaaccc tggcagccgc tattgtgatg cgatccggct ggcagccagg aacaccgttg
     9961 ctcgatccga tgtgtggttc cggtacgttg ctgattgaag cggcgatgct ggcgaccgat
    10021 cgcgcaccag gcttgcaccg tgggcgttgg ggctttagcg gctgggcgca gcatgatgaa
    10081 gctatctggc aggaagtgaa agcggaagcg caaactcgcg cccgtaaagg cctggctgag
    10141 tatagctctc atttttacgg ttcggacagc gacgctcggg tgattcaacg tgcacgcact
    10201 aacgcccgtc ttgcggggat tggtgaactg atcacctttg aggtgaaaga tgtcgcgcaa
    10261 ctgaccaatc cgctgccgaa agagccgtac ggcacagtgt taagcaaccc gccatacggg
    10321 gaacgtctgg acagcgaacc ggcgctgatt gcgctgcata gcctgctggg tcggatcatg
    10381 aaaaaccagt ttggtggctg gaatctctct ttgtttagtg cctcgccgga tctgctaagc
    10441 tgcttgcagc tgcgtgcaga caaacagtac aaggcgaaaa acggcccgct ggactgcgta
    10501 cagaaaaatt accacgttgc cgaaagcact ccagacagca aaccggcgat ggtagcggaa
    10561 gactacgcca accgcctgcg taagaacctc aaaaaattcg agaagtgggc tcgtcaggaa
    10621 gggattgaat gttaccgcct gtatgacgcc gatctgccgg aatataacgt tgccgttgac
    10681 cgttatgccg actgggtggt ggtgcaggag tatgcgccgc caaaaactat tgatgcgcac
    10741 aaagcgcgtc agcgtctgtt cgatattatc gctgcaacca tttcggtact ggggattgcg
    10801 ccaaacaaac tggtgctgaa aacccgtgaa cgtcagaagg gcaaaaatca gtaccagaaa
    10861 ctgggcgaga agggcgagtt tctggaagtt accgaatata acgctcactt gtgggtgaac
    10921 ctgacggatt atctcgatac tggtctgttc ctcgatcacc gtatcgcccg tcgtatgctc
    10981 ggccagatga gcaaaggcaa agatttcctc aacctgttct cttataccgg cagcgccacc
    11041 gtgcatgcgg gattaggcgg tgcacgcagc accaccaccg tggatatgtc gcgtacttat
    11101 ctggagtggg cagaacgcaa cctgcgtctg aatggcctaa ccgggcgtgc gcatcgcctg
    11161 attcaggccg attgcctggc gtggctgcgt gaggcaaatg aacagttcga tctgatcttt
    11221 atcgatccgc caaccttctc taactcaaaa cgaatggaag atgcgtttga tgttcagcgc
    11281 gatcatctgg cgctgatgaa agatttgaaa cgtctgctgc gtgcaggtgg gacgatcatg
    11341 ttctcgaaca acaaacgtgg cttccgtatg gatctcgacg gcctggcaaa actgggactg
    11401 aaagcacaag aaattacgca aaaaacgctc tcccaggatt tcgcccgtaa ccgccagatc
    11461 cacaactgct ggctgattac cgcagcctga aaggaatagt aatgtcatta atcagtatgc
    11521 atggcgcatg gctgtcgttc agcgacgcgc cgcttctcga taacgcagaa ctgcatatcg
    11581 aagataacga acgtgtttgt ctggtgggcc gcaacggcgc aggcaaatcg acgttaatga
    11641 aaatcctcaa ccgtgaacaa gggctggatg acggtcgcat tatttacgag caggatttga
    11701 ttgtagcgcg tctgcaacag gatccgccgc gtaacgttga gggtagtgtt tatgatttcg
    11761 ttgccgaagg cattgaagaa caagcggaat acctgaaacg ctatcacgat atttcgcgcc
    11821 tggtgatgaa cgacccgagc gagaaaaatc tcaacgaact ggcgaaggtt caggaacagc
    11881 tggatcacca caacctgtgg cagctggaaa accgcatcaa cgaagtgctg gcgcaactgg
    11941 ggttagatcc taacgttgcg ctgtcgtcgc tttccggcgg ctggttgcgt aaagcggcat
    12001 taggacgcgc gctggtgagc aatccgcgcg tattgctgct ggacgaaccg actaaccacc
    12061 tggatatcga aaccatcgac tggctggaag ggttcctcaa aaccttcaat gggacgatta
    12121 ttttcatctc ccacgaccgt tcgtttatcc gcaatatggc gacgcgcatt gttgatctcg
    12181 atcgcggcaa gctggtgacc tatccgggca attacgatca gtacctgctg gaaaaagaag
    12241 aagccctgcg cgtggaagaa ctacaaaatg ccgagtttga tcgcaaactg gcgcaggaag
    12301 aagtgtggat tcgccagggg atcaaagcac gccgtacccg taatgaaggc cgcgtacgcg
    12361 ccctgaaagc gatgcgtcgc gagcgtggcg aacgtcgcga agtgatgggt accgcaaaga
    12421 tgcaggtgga agaggccagc cgctccggta agatcgtttt cgaaatggaa gacgtttgct
    12481 accaggttga cggaaagcaa ctggtgaaag atttttctgc ccaggttcta cgtggcgaca
    12541 aaattgccct gattgggccg aatggctgcg gcaaaaccac gctgctgaaa ctgatgctcg
    12601 gtcagcttca agcggacagc gggcgtattc acgttggcac caaactggaa gtggcttatt
    12661 tcgatcagca ccgcgcggaa ctggatcccg ataaaacggt gatggataac cttgccgaag
    12721 gtaagcaaga ggtgatggtt aacggcaagc cacgccacgt tttgggctat ttgcaggact
    12781 tcctgttcca tccgaaacgg gcgatgacgc cagtacgtgc gctttctggc ggtgagcgga
    12841 accgcttgct gctggcgcgt ttgttcctca aaccaagcaa cttattgatt cttgacgaac
    12901 cgaccaacga tcttgatgtc gaaacgctgg aactgctgga agaactgatc gacagctacc
    12961 agggcacggt attgctggta agccacgatc gtcagtttgt cgataacacc gttacagaat
    13021 gctggatctt cgaaggcggc ggtaaaattg gtcgttatgt cggcggttat catgatgccc
    13081 gtggtcagca agagcagtat gtggcgctca aacagcctgc ggtgaaaaaa aacgaagaac
    13141 ccgccgcgcc aaaagcagaa actgtaaaac gcagcagtag caaactaagc tataaattgc
    13201 agcgcgaact ggagcagcta ccgcaattgc tcgaagatct ggaggcgaag ctggaagccc
    13261 tacagacgca ggtggcggat gcttccttct tcagtcagcc gcatgagcag acacaaaaag
    13321 tgctcgccga tatggctgct gcagagcagg agctggagca agcctttgaa cgctgggagt
    13381 atcttgaagc gttaaaaaat ggtggctgat cgcaaaatag tcgatttaaa cagagacagc
    13441 tgacaacggt aaaattgcct gatgaactgg gtttatcagg catgaaaaca aacactttgt
    13501 tagtcatctg taaaatagcg catcattaag gagtaccaat gtgcgaacat catcatgccg
    13561 cgaagcacat cctgtgctcg cagtgtgaca tgctggtggc gttaccgcgc cttgagcatg
    13621 gtcagaaagc ggcatgtccc cggtgtggca caacgttaac cgtggcgtgg gatgcccccc
    13681 ggcagcgtcc gaccgcctat gcgttggctg cactgttcat gctgttgctg tccaacttgt
    13741 ttccttttgt gaatatgaac gttgcaggag ttaccagtga aattacatta ctggaaattc
    13801 ccggcgtgct tttttctgag gactacgcca gcctcggcac ctttttcctg ttgtttgtgc
    13861 aactggttcc cgcgttttgt ctgataacca ttctgttact ggtgaatcgc gcggaattac
    13921 cggtccgttt aaaagagcaa ctggcacggg tgctttttca actcaaaacc tggggaatgg
    13981 cggagatttt cctcgcgggt gtgctggtca gtttcgttaa actgatggct tacggcagca
    14041 ttggcgtagg cagcagcttt ctcccctggt gtttattttg tgtcctgcaa ctgcgcgctt
    14101 ttcagtgcgt tgatcgtcgc tggttatggg acgatatcgc cccgatgcca gaactgcgcc
    14161 agccgctaaa accaggcgtc acggggatac gtcaggggct gcgttcgtgc tcctgttgta
    14221 cggcaatcct tcctgctgat gaacccgtgt gcccgcgctg tggtactaaa gggtacgttc
    14281 gacgtagaaa cagcctgcaa tggacactcg cgctgcttgt tacgtccatc atgctgtatc
    14341 ttccggctaa tattttgccc atcatggtga cggatttatt aggctcgaag atgccatcga
    14401 cgattctcgc tggggtcatc ctgttatgga gcgaaggctc ttatcccgtc gctgcggtga
    14461 tctttctggc cagtattatg gtgccaacgt taaagatgat cgccatcgcg tggctgtgtt
    14521 gggatgccaa agggcatggc aagcgcgaca gtgaaagaat gcatttgatt tatgaagttg
    14581 ttgagtttgt aggccgctgg tcgatgattg acgttttcgt tatcgcggtg ctctcggcgc
    14641 tggtgcgtat gggaggttta atgagtattt atccggcaat gggtgcatta atgtttgctt
    14701 tagtcgtcat aatgacaatg ttttctgcta tgacgtttga cccgcgtttg tcgtgggatc
    14761 gtcaacctga atcagagcat gaggagtcct gacagtatgg aatctaataa tggggaagcc
    14821 aaaatccaga aagtgaagaa ctggtctccc gtgtggatat ttcctatcgt cacggcgctc
    14881 attggggcct gggttctttt ttatcattac agccatcagg gaccggaagt gaccctgatc
    14941 accgcgaatg cggaaggaat tgaaggtggc aaaaccacca ttaaaagccg tagcgttgac
    15001 gtcggcgtgg ttgaaagcgc cacactggct gatgatttga cacacgttga aatcaaagcg
    15061 cggctgaatt ccggtatgga aaaattgctg cataaagata ccgtcttttg ggtggtgaaa
    15121 ccgcagattg gtcgagaagg gattagcggt ctgggaaccc tgctgtctgg ggtttatatc
    15181 gaactgcagc caggcgcgaa aggcagcaaa atggataaat acgatttgct ggactcgcca
    15241 ccgttggccc cgcctgatgc gaaaggtatc cgtgtggttc tcgatagcaa aaaagccggg
    15301 cagctctcgc caggagatcc ggtgctgttc cgtggctatc gggtaggttc ggttgaaacc
    15361 agcaccttcg atacgcaaaa acgcaatatc agttatcaac tgttcatcaa tgcaccttat
    15421 gaccgactgg tgaccagcaa tgttcgcttc tggaaagata gtggcattgc ggttgatctg
    15481 acgtcagcgg gaatgcgtgt ggagatgggc tcattgacaa cgctgctgag tggcggtgtc
    15541 agctttgatg tgccggaagg tctggattta gggcagccag tggcaccgaa aacagctttc
    15601 gttttgtatg atgatcagaa gagcattcag gattcgttgt acaccgatca cattgattat
    15661 ctgatgttct ttaaagattc ggtacgcggt ctgcaaccgg gagctccggt agaattccgg
    15721 ggtattcgcc tcggtaccgt aagcaaagtg ccattctttg cgccgaatat gcgtcagaca
    15781 tttaacgatg attaccgtat tccggtgctg attcgtatcg agccagagcg gctgaaaatg
    15841 cagcttggcg aaaatgcgga tgttgttgag caccttggcg aattgttgaa acgtggttta
    15901 cgcggatcgc tgaaaaccgg aaacctggtc actggcgcac tgtatgttga tctcgatttc
    15961 tatccaaata cgcctgcaat aaccggtatt cgtgaattta atggttatca gattatcccg
    16021 accgttagcg gcggcctggc gcaaatccag caacgactga tggaagcgtt ggataagatc
    16081 aacaaactgc cattgaatcc gatgattgaa caggcaacca gtacgctttc tgaaagtcag
    16141 cgcacaatga aaaacctgca aacgacgctg gatagcatga acaagatcct cgccagccag
    16201 tcgatgcagc agttgccgac ggatatgcag tcaacgttgc gtgaattgaa tcgcagcatg
    16261 cagggcttcc agcccggctc cgcagcctac aacaagatgg tggcggatat gcagcgcctt
    16321 gatcaggtgt tgcgagaact gcaaccggtg ctgaaaacgc tcaatgagaa gagtaacgcg
    16381 ctggtatttg aagcgaagga caaaaaagat ccagagccga agagggcgaa acaatgaaaa
    16441 agtggctagt gacgattgca gcactgtggc tggccggatg cagctccggc gaaattaata
    16501 aaaactatta ccagttacct gtggtgcaga gcggtacaca aagtaccgcc agccagggca
    16561 atcgcctgtt atgggtagag caggtcgctg tacctgacta tctggcgggg aatggtgtgg
    16621 tttatcaaac cagtgatgtg aagtatgtga ttgccaacaa caacttgtgg gccagcccgt
    16681 tggatcaaca gttgcgcaac accctggtag ccaacctgag cacgcaactg cccggctggg
    16741 tggttgcctc ccagcctctg ggaagcgccc aggacacgct caatgttacc gtaacggagt
    16801 ttaacggtcg ctatgatggc aaggtcattg tcagtggtga gtggctgttg aaccaccagg
    16861 gacaactgat caaacgtccg ttccgtctgg aaggagtgca aactcaggat ggttacgatg
    16921 agatggttaa agtgctggcc ggtgtctgga gtcaggaagc cgcttctatt gcacaagaga
    16981 taaagcgtct accttaatta taaagatttg taaatataac cgtctccggt atgttgcctg
    17041 aggcggtttt tttgtttcta acgtgcggaa aaatttgttc ctcttcacat tttttgtaca
    17101 accgacatgc ccgtgtagct cacaaatatg acagtggcgt gaattttgcg cattgacggc
    17161 agttatgatt cgcggtattg cttaactgtg attgcacatt tagtaatcac tgttttcttt
    17221 tccaccagaa accagtatga gggaaacgag gcatgaagag acaaaaacga gatcgcctgg
    17281 aacgggcaca tcaacgtggt tatcaggccg gcatcgccgg acgctcaaaa gaaatgtgtc
    17341 cctatcagac gctgaatcaa aggtcacaat ggctgggagg ctggcgagaa gccatggcgg
    17401 acagggtagt aatggcctga ttctgtctct ttaaaaagaa acctccgcat tgcggaggtt
    17461 tcgccttttg atactctgtc tgattataat cagaaggcag acgtatcctg gaacagaccg
    17521 actttcaggt cgctggcggt atagatcaga cgaccatcaa ccagcacttc gccatccgcc
    17581 aggcccataa tcagacgacg gttaacaatg cgtttaaagt gaatacggta ggtcactttt
    17641 ttcgctgtcg gcagtacctg accagtgaat ttcacttcgc caacgcccag cgcgcggcct
    17701 ttaccttcgc cgcccagcca gccgaggtag aaccctacca gctgccacat tgcgtccagg
    17761 cccaggcatc ccggcataac cggatcgcca ataaagtggc atccgaagaa ccacagatcc
    17821 ggattgatat ccagttctgc ttcaacatac cctttgtcga agttaccacc cgtttcggtc
    17881 attttgacca cacggtccat catcagcatg ttcggtgctg gcaattgcgg gcctttagcg
    17941 ccaaacagtt caccgcgacc agaggcaaga aggtcttctt ttgtatagga ttcgcgttta
    18001 tctaccatgt tctctgtaag ccttatttta ttgaagcacg caggatagct aacacgtgta
    18061 cgctgaacaa gtccgatcag ttcggaataa accagttcag ccaacgtaat ggccatggaa
    18121 aacggtgacg tccttcctgt tgcgatgctt gcgcgatacg ttcctggatg gtttgcatca
    18181 gcgtcgtttg gccttcgcca tcccacacca gatttaataa taacggcagt gcgtcagtca
    18241 catcgtctac tgcccagatg gtgaatttgc cttcttctac cgctttcacc agttcactgt
    18301 gaagacttaa atggcgaacg ttagctgtgg ggataatgac accttgtttc ccggttaact
    18361 cacgttgctg gcaaatagca aagaagcctt cgattttctc atttaaacca ccgaccggct
    18421 gggcgcgacc gaactgatcg actgaacctg tgatagcgat actctgattc accggcacat
    18481 cggcgagggc gcttatcagg gcgcagagtt cagccatcga ggcactatct ccatcaactt
    18541 cactgtatga ctgctcaaat gtcagcgatg ctgagaaggg gatctgttgc tcaagctgta
    18601 gttccgacat caggaacgct tgcatgatca tcatcccttt cgcatggata ttgccgccaa
    18661 gctccgcttt gcgttcgatg tcggtgaatt caccatcgcc aatatgcaca acgcagctaa
    18721 tgcgagaagg ttcgccaaaa gcgcgtggat gacccggaaa ttcaatgacc gaaagggcgt
    18781 taatttgccc gatgcgttcg ccttcggttt caatcaggat ttgctcctga aggatctcat
    18841 cctgcatacg ttcagcgagg aaaccttcgc gccattcacg ctgctgcagc attaagttta
    18901 gctgctcgcc ggagaaggtg tcgccatcac acagggaggc gacctcttta cactggcgga
    18961 ggatccactg cgggctaagc ggaagtgttt cttgttcacc ggtgtagcgt gctgcttcgc
    19021 ggataagtac cggccaggca tccgctcccg gggcaggtaa gtgattatgt ctggcggtaa
    19081 atgtcaccca gcgacaccac tgggttactg actccgcatc gacaatctgc agagtatctt
    19141 caaattcgct ataaatagcc tgctctgaaa gctctggctc catctcctgg aaatcagcca
    19201 atgattcgcg ttcgcctacc agaatgactt tcagcttcaa tggcatcgaa ggcacagaga
    19261 cggggagagg gcgcgactca tcaaacgcaa cccagtcaaa acgctcgcgg ttaacgatat
    19321 ttttcagccg catccacagc agaggttgcg ccagcagtgt acgcaaagag atgatgagaa
    19381 taccgccatt tgcctgatgc accagaccag gctgcagggt aatgtcgcca ttaaactggc
    19441 gcaggcagcc aaagagttgc tccgcttcta cccagtcggc agcgacaact tgcgttaaag
    19501 tcgcaaaatt atcatctgca ctcactgcgt gacgtaagcg gatggagtgg ccggaaacct
    19561 catagtgacc gcccaccagt tggcctgcat cgctttgtag cgtacgcgcg gcattggcaa
    19621 taagattcag atactcagac tcttccgggg ccttcgccag cataaaagag gaggatgctc
    19681 gcgtatgcag caactgctcc agcgcaaatt gcagccgcgg ttgagtatca ctgaataaag
    19741 gatcgttttc gtcaatcaaa tgtggctgag caaatatttc ctgatagcta tcggtatcag
    19801 gaaccaggtc acgccatgca agtttcgtaa tggtcaaagt tgatgttttt tagtctgttg
    19861 tcaaagccgc gattataccg taaccggcac tacagcacac gtagaaagca ccgacaatac
    19921 tcctggcatg ggcgttaaag ctcacaggat ggagattctt ttcttcactg gcctaaaaag
    19981 ctgatattct gtaaagagtt acacggtaac attgagatcg ctatgaaata tcaacaactt
    20041 gaaaatcttg aaagcggttg gaaatggaag tatctggtga agaagcatcg cgaaggggag
    20101 ttaatcaccc gttacataga agccagtgcc gcccaggaag ccgtcgatgt gttgctctcg
    20161 ctggaaaatg aacccgtgct ggtaaatggc tggattgaca agcatatgaa tccggaactg
    20221 gtcaatcgca tgaagcaaac cattcgggca agacgaaagc gccattttaa tgcggaacac
    20281 cagcatacgc gcaaaaaatc gatcgatctg gaatttattg tctggcaacg tctggctggt
    20341 cttgcgcagc gtcgcggtaa aacgctttct gaaacgattg ttcagctgat tgaagatgcg
    20401 gaaaacaaag agaaatacgc gaataaaatg tcttctctga agcaggatct gcaggcattg
    20461 ctgggtaagg aataactgac gaaagtcagt tcaatttact aaaggcaaaa aaaccccgca
    20521 gcagcggggt ttttctacca gacgagaact taagcttgcg gctgagttac aacgtctttg
    20581 atacctttaa cttcgatctc tacgcgacga tccggagcca ggcagtcgat cagtgcagca
    20641 cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc cataccacgt
    20701 gcggagatct tgtctgccgg gatacctttg gagatcaggt aatcaacaac agactgagca
    20761 cggcgctcgg acagaccctg gttgtaagcg tcagaaccga tgcggtcggt gtaacccaga
    20821 acaactacgg aaccgtcttt cggatccagg ttgctcagct ggctgtacag ctgatccaga
    20881 gcagcctgac cttccggttt cagggttgct ttgttgaagt tgaacagaac gtcagacttc
    20941 agagtgaagt gcttggtctg tacttccggt gccggagctg gagccggagc aactactgga
    21001 gctgcttcgc cctgaccgaa acggtaggaa acacccaggc tcagcatgcc gttgtccgga
    21061 cgagtgccga tggtgtgtgc gtcaccgatg ttgttggtcc actggtattc cagacgggta
    21121 gcgatttcag gagtgatcgc gtactcaaca ccgccagcga agaccggaga aacgccggtg
    21181 tcgtggtttt taccataaac gttggattta gtgtctgcac gccataccat accacccaga
    21241 cgagtgtaga tgtccaggtc gtcagtgatt gggtaaccca gtttagcggt cagttgaacg
    21301 ccctgagctt tgtatgcacc gttttcaacg ctgcctttgt acggcatacg acctaaccag
    21361 tcgtaaccca tttcaaagcc aacatacggg ttaacctggt aaccaccaaa agcaccagcg
    21421 cccagttggt tttcatgggt cgggccattg ttgttgatga aaccagtgtc atggtactgg
    21481 gaccagccca gtttagcacc agtgtaccag gtgttatctt tcggagcggc ctgcgctacg
    21541 gtagcgaaac cagccagtgc cactgcaatc gcgatagctg tctttttcat tttttgcgcc
    21601 tcgttatcat ccaaaatacg ccatgaatat ctccaacgag ataacacggt taaatccttc
    21661 accgggggat ctgctcaata ttaactctac cgatatcttc ggcttatgcc gagcacccct
    21721 ggcgatgtaa agtctacaac gtagttgaaa acttacaagt gtgaactccg tcaggcatat
    21781 gaaaaaaaag tcttgtataa ggtatgttta atcttttttg tcagcgacaa tttacagaag
    21841 agaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa aattagtcac gactgaaagc
    21901 attggctggg cgacaaaaaa agttccagga ttaatcctaa atttacttaa tgatacaaat
    21961 tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg agaggatgcg cttaccggac
    22021 gcataataaa ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt
    22081 cttcagtcaa atcatctgcc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag
    22141 cgcgaaccat tgactccaca gtgtggcaag gggagagctg gctaatctgc attactttcg
    22201 ttaagggtag cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc
    22261 agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg
    22321 tcatcatggg ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt
    22381 ccgtagagac acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat
    22441 agcctgaagt gtacataatc aatccagccc ctgtgagtta ctgtatggat gtacagtaca
    22501 tccagtgaca acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg
    22561 tgacgggaaa acataaatta atcttgccgc ttaagaataa gttgcctatt ttcgtagtta
    22621 acggatccgt taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa
    22681 agcctctcct ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt
    22741 gaataccgat cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg
    22801 gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa
    22861 tatccgcctg tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat
    22921 cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct
    22981 ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg
    23041 cccaatatgt cttttcatct ggaagcgatt ctcggggagg tggggattaa ggatgtacgg
    23101 gcgttacgta tacttggggc aaaaatgtgt tggttgcgac tgcggcagca aaacagtctg
    23161 gtgacagaaa agattctgtt tatgcttgaa ggtgccatta tcggcattca tgaagctgcg
    23221 ctcccggtgg cacgccgcca ggagcttgca gaatgggctg actctcttac gccgaaacag
    23281 gagtttcctg cggaacttga gtaatctggc gttgcagacg accaatctca ggcagtaatg
    23341 caatcaatag tccaacttgt tgtacgacca gaggttcttt gctgtctgcc cgtggttcaa
    23401 gttgctgcat ccgctgtttc aggctagcta atgcctcatt gacgcgttct tcatcagcag
    23461 gttgatgatg taacgcgtca tcaacatagc aaactgcgtc atcaagaaac gccagaattt
    23521 caggattagt taactgctcc cggtgagcac cgagggctga gatatagctg gtaaacgtat
    23581 ggttaaggca cagcaaccga aacgcggctt cgcgaatttg cggggtaacg ttcggctcgc
    23641 tggacatatt tgataccacc gacgccagct cagcatcacg gttgtgtgca tcgcggcggg
    23701 caatacgata cgccagacgg ttatcacgcc cctgatggta ttgctccagt atggcatcga
    23761 gataccgaca gttggcctct gtggcgcgtt cgagcatgcg cggcagattg cgaaactgcc
    23821 agtcaggcca gatgtagctc actgccgccc acgcaatggc acaaccaatc agcgtatcga
    23881 ttacgcgagg taacgctact tcaaaacctt cacccagtaa gttaaaacac agtagcacca
    23941 aaagtgtgat gaacatcgtt gcatgagcgt attgcacgtt acggaaggca aaaaagagca
    24001 cgccggtaat aaccagcagc accagctgcc cttccagtga tggcacaaac cacagcacag
    24061 gaatgccaat ggcgatacct accagcgtac caataatcct taacttcagg cggtggcgcg
    24121 tggcgttata gtttggctgg cagacaaaca aacttgtcag caagatccaa tacccgtgat
    24181 gcattccggt tatctgaatg atggcgtagc cgaagcacaa caccagcgac attcttaccg
    24241 catgacggaa gagggcggat tccggcgtga agtgacggct aagacgcagc cagatatcac
    24301 tcaacccgtg cgggctgtca tcagcgagct cattttcgtc attattatgg ggtagtgcct
    24361 gggcctgttc tgattcaatt gttgccagtt gggcatcaat ggcgcgtaaa ttgttcagca
    24421 aaaatcccag tgttttgagt aaatcggcgg gtgcgccgtt atcgcgcatc cgctccagcg
    24481 cagcatcaat atgcgtaaaa gcgcgctcaa aatgcggatc atgttgataa ggctgacgca
    24541 acaaaataca gcgtgacagt tgctggcacg cctggccctg catcgacatc agccgctgaa
    24601 aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa tgtttgatac tgaatatgag
    24661 aagagctggc acgctcgtga atatcctgtg cgacaaagta ataatgcagc gtgcgacgcg
    24721 ttccccgttg accacgatcg ccacgtaagc gggtcagcag cgagagtttc gtctgattca
    24781 atgtcgccat cagcagaccg ttggcgagag ccaaatcgta cagcggtgcc tggctttcat
    24841 cttcaatatc aggatcaaac atgcgcgact tgagctcaag ataacgcgcc agttgttcat
    24901 agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa cagcagatga ccaataagtg
    24961 tcaggacgtt gtaccagacg gcaccggcca gcagatacat cggctgctga taccagtgct
    25021 catacagtga tgttcccaac atagtgtaaa tggcgatcag caatgcaccg aaggcaattg
    25081 ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc gctggtagag agcgttaagc
    25141 caatcgcaaa tagccagggc cagggaaaca gcaattctac tgaggccgag gcgataaaaa
    25201 agcagaacag cgtaatgatg aggttacgca aacgtcccgc cagtcggtca tcgagatcgg
    25261 tcagcgccgc tgccaccatc cccagcgtta gcggaatcgt cagttttaca tcacccagcc
    25321 accacggaaa cgctgtggtt ccacaaagcg caataaaaat acgcgcgtaa tacagccagg
    25381 cgctgttcca ggtatagcgt ttgagcaaag gacttagcat aaaggccata ttagtggatt
    25441 attcaaaacg acgacgtgca ttggcttcac gcgcagcttg cgctgtttct accgatacca
    25501 cgcgacgacc aaccggccat agtgcaatag cggcaatttt aaagttcgca atgccgacag
    25561 gaatgccaat gattgaaata cattgtgcga tgcccgttgc aatgtgcatc aggcataacc
    25621 accagccaaa gaaaatcagc cagaaaatat tcaataccgt accgccagta ttcagcagca
    25681 catttttgcc agccgggttc agttcatcga catgaatagc ttcattgcca taaggcacca
    25741 gagacagttt agtgatctcc cagcaggatc gtgtcagcgg taaggtaaaa atcagcgcaa
    25801 tactgaccag agtcgccaac agccagccca gagtggtggc aaatccgcca agcacaaagt
    25861 tcagaatgtt caaaacggta cgcataaaac ctcgctttac tgtggttttc agtaatgggc
    25921 ggcaattgta acgttttttt gggctggagc acgttttctc tgacggttac actgataaga
    25981 aataatttcg tgtggatcta cagagtcatg gaactgaaag cgacaacgct tggaaaacgt
    26041 ctggcacagc acccttacga tcgggcggtg atcctcaatg ccgggattaa agtctccggc
    26101 gatcgccacg aataccttat tcctttcaat caattactgg cgattcactg taagcgcggt
    26161 ctggtatggg gcgagctgga atttgtactg ccggacgaaa aagtggtgcg tctgcacggt
    26221 accgaatggg gcgagacgca gcgtttttac catcatcttg atgctcactg gcggcggtgg
    26281 agtggcgaga tgagcgaaat tgcgtctggt gttttacgcc agcaactgga tttgattgcc
    26341 acgcgcactg gagaaaataa atggctgacg cgtgagcaaa cctctggcgt tcagcaacaa
    26401 atccgccagg ctttgtcggc gttgccgttg ccggttaacc gactggaaga attcgataac
    26461 tgccgtgagg cgtggcgtaa atgtcaggcc tggttgaaag atattgaaag cgctcggttg
    26521 cagcataacc aggcgtatac cgaagccatg cttaccgagt atgcggattt tttccgccag
    26581 gtcgagtctt caccgctgaa tccggcgcag gcccgggcag tcgttaatgg cgagcattct
    26641 ctgttagtgc tggcaggtgc aggaagcgga aaaacgtcgg tgctggtggc ccgtgcaggc
    26701 tggttgctgg cgcgtggtga agcgtcccct gagcaaattt tattgctggc gtttggtcgc
    26761 aaagccgctg aagagatgga cgagcgtatt cgcgaacggc tacataccga agacattacc
    26821 gcacgcacgt ttcatgcgct ggcgctgcat attattcagc agggcagcaa aaaagttccg
    26881 atagtcagca aactggaaaa tgataccgct gcccgtcatg aactctttat tgctgagtgg
    26941 cgcaagcaat gcagcgaaaa gaaagcgcag gcgaagggct ggcggcaatg gctgacggaa
    27001 gaaatgcagt ggtcagtgcc agaaggtaac ttctgggatg atgaaaaatt acagcgtcgt
    27061 cttgcctcac gcctcgatcg ttgggtaagt ctgatgcgga tgcacggtgg tgcacaggca
    27121 gaaatgattg ccagtgcacc cgaagagatt cgcgatctgt tcagtaaacg tatcaagttg
    27181 atggccccgt tattaaaagc ctggaaaggt gcgctgaagg cagaaaacgc tgtcgatttt
    27241 tcgggcctta ttcatcaggc gattgtgatt ctggagaaag gtcgctttat cagcccgtgg
    27301 aagcatattc tggttgatga atttcaggat atctcgccgc agcgggcagc gttgttagcg
    27361 gcattacgca agcaaaacag tcagacgacg ttgttcgccg ttggtgatga ctggcaggcg
    27421 atttaccgat tcagcggtgc gcaaatgtcg ctcaccaccg ctttccatga aaactttggt
    27481 gaaggcgaac gctgtgattt agacacgact taccgtttta acagtcgtat cggtgaggtg
    27541 gcaaaccggt ttattcagca gaacccaggc cagctgaaaa agccgctaaa cagcttaacc
    27601 aatggagaca aaaaagccgt cacgttattg gatgagagtc aacttgacgc tttgctggat
    27661 aagctctctg gttatgccaa accggaagag cgcattctga tcctggcgcg ttaccatcac
    27721 atgaggcctg ccagcctgga aaaagcggca acacgctggc cgaagttgca aatcgacttt
    27781 atgaccattc atgccagcaa agggcaacag gcggattacg tcctcatcgt tggcttgcag
    27841 gagggaagtg atggttttcc ggctgcggcg cgggagtcga ttatggaaga ggcgctactg
    27901 ccaccggttg aggatttccc ggacgctgaa gaacggcggt taatgtacgt ggcgctgacc
    27961 cgggcacgtc atcgggtatg ggcactgttt aacaaagaga atccctctcc ctttgtggaa
    28021 atactgaaaa atctggatgt gccggtggcg agaaaaccgt aagaaacagg tggcgtttgc
    28081 cacctgtgca atattacttc agacggtccg cgagataacg ctgataatcg gggatcagaa
    28141 tatcgaccgc gtcgttgaaa tgcggcgact ggataatgaa gtctgccgtt gccacgttgg
    28201 tggcgaccgg aatgttccat accgtcgcca gacgcagcaa ggctttcacg tcaggatcgt
    28261 gcggcacggc atttagtgga tcccagaaga aaatcaatac atcaattttc ccttctgaga
    28321 tcaatgcgcc aacctgctgg tcacccccca ttgggccact caacatcgcg ttgacgttca
    28381 tgccggtcgc gcgggaaatt aagttaccgg tagtgcctgt tgcatacagt acgtgttgtt
    28441 ccagtaacgg ttgatgccgt tccacccagc tcatcagcat ttgtttgcag tgatcgtgtg
    28501 ccaccagcgc aatatgtttc cgcgcaggta aagtgcgagt cgtcagttcc ataatgtaca
    28561 tccgtagtta actttcctac agattactgt aagcacttat cgctgcaaga taaagaccga
    28621 aaaagcctgc gcacaggcac aaaaatctca ggaagatggt tgtttttccg cccattgcag
    28681 gaaagtattt cgcgtttgtg ggtcagccag tttaaaccaa tacttcagcc gttgttctgt
    28741 gagcacctga gactgcggtg gaatagcatc cagctcggaa acacccgaaa gcagcgtact
    28801 gtcatctgcc atcatcgtgg caaattgcgg tagtgaagcg cgtttcgcgg atttgttata
    28861 gcgttcaacc tcgacctcat aatcaatcgt ttttgccgtt gaggtaatgg cgagaatatc
    28921 cagctttacc ggaatcggcg tcgcatcgcc atccaacaat tcaaggcgcg gcgcggcatc
    28981 aaaatggtta gcttcccgct cattctccag gcgaggcagg cgaaaattga cctggttgat
    29041 gagctgggta ttaaaactga ccaccagcgg tggggagata tacagccgtt cttcactatt
    29101 ggaaagatga atcgtcttct caacgcgaaa cactaactga tgcggtccat tatccagttc
    29161 aatgctgtcc gcaccgcgca gcagagagct ggaaaccttc ttgccgtcga gtaccagaag
    29221 atcgacatcg gttgaaagcc gcagcgtggt ggcaaaaacg gataccggca aacataatgc
    29281 aatcaaggtg gtcacgatgc cggttttcat aggtagctcc tgtcaaaaga ccgcccgaat
    29341 aataatgtgt caaaattttt ctttaatcat acttactaac atatagacat atttcccgct
    29401 tttgctctca ttcattcgta ttagctgcat ggttggcatg tcggctttgt cgtacacttt
    29461 gtgaaacagt ctggaggaaa taatgaaaga aaccgatatt gcaggcattt taacgtccac
    29521 tcacaccatt gcgctggtgg gggcaagcga caaacccgat cgtcccagct atcgcgtgat
    29581 gaaatatcta ctcgaccagg ggtatcacgt cattccggtt tcgccaaaag ttgccggcaa
    29641 aacgctgctg ggccagcagg ggtacggcac gctggcagac gttccggaaa aagtcgatat
    29701 ggtcgatgtt ttccgtaatt cagaagcggc gtggggcgta gcgcaggaag ctatcgccat
    29761 tggcgcgaaa accctctgga tgcaactggg ggtgattaat gagcaagcgg cagtactggc
    29821 acgggatgcc gggttgaatg tggtgatgga tcgctgcccg gctattgaga tccctcgcct
    29881 gggcctggcc aaataaaaaa tccccggaag gcaaaaacct tccggggatc tgttcaggga
    29941 ttagttacgc agacgcgggg cctggagttg tttgcggatg gtctgcgcca gctcatccat
    30001 ggatggctgt tcaggatgtt catcctgcaa ttcgctactt agctgggctt cagccaggta
    30061 ggtatgaacc ggtaggccgt tatcgtcctc catcaccacg tgataccacg gagcggcgcg
    30121 aagctcgtca ttcaccgcca gctcatcagg cgacggttca gagagcgaat aaaccgggtc
    30181 gatatccacg accactccga gataacctaa cagggaatgg cggacctgct ggccgatacc
    30241 gaatttgctg gcaatcatag tcacctcccg ggaaatcttt acctttgata taagggtaaa
    30301 attccacatt tcaagttaca tgacgcgaca ggcaaaccct ttcagatata gcccttccgg
    30361 ataggtagcg atcaccggat gatcggccgc ctgacggaac tgctctataa attgtacatc
    30421 acgaccggca tcaattgcgg catccgcgat gattttctga aataaatcgc tggtcatcag
    30481 accggaacag gagaaagtca ggagaatacc gccttcattc agcagctgaa tcgccagcat
    30541 gttgatatct ttatagccac gacacgcgcc catcaactgg cttttattct caacaaactt
    30601 cggcgggtcc atcacgataa cgtcaaattt ttcaccgcga tcgcgatagg tacgcagcaa
    30661 tttaaagaca tcatcacgga caaactcagc cttgctcaga tccagtttgt tcagctcaac
    30721 gttctgccgt gcaatatcca gtgcttcctg ggaggtatca acgctgacaa cctggctgca
    30781 accgcccatc agtgccgata cggcgaaacc accggtatag gagaaacagt tcagtacgcg
    30841 tttattttca acgtagcggc gggtagccag gcggctgtct cgctggtcca ggtagtagcc
    30901 cgttttgtgt ccgtgctgta tgtccaccag cagcttcatg ccatgttctt caatcggcag
    30961 cagggcaggc ggcaactcac cggtgacgag gccctgggtc agctccatcc cttctttttt
    31021 acgtaccgca acatcgctgc gatcgtaaat cgcacattcc gggtacagcg tttgcagggc
    31081 actaattaat gccgcgcgct gatattctgc gccagcactc agcagttgca gcaccagaaa
    31141 attaccgaaa cgatcgatag taatacccgg caggccatca gattctccgg cgattaaacg
    31201 atagctgtcg aggccatctt tttgcgccag ccagtcacgc catttttgtg cttgttgcaa
    31261 acggcgggaa aaaaaagcaa tgtcgataga ctcagacggg tcaaacgtcc agacgcgcgc
    31321 ccggatttgc gaagctggcg aataagcgcc gcgtgctaac cattttccct gatgatcaac
    31381 aatatcgatg gtttcaccga ggctggcttt accttccata cgggcaacgg ccccggaaaa
    31441 gacccacgga tggcgacgaa gtaatgattt ttcgcgccct ttggctaaca ctaaacgtac
    31501 actcataatt tacttttctg tcgatgcaaa gaaatgggcg tcattgtccg gagttttcca
    31561 tggatttgca acgcgtccat taaggataag gagagcagca atgtcgaaag tctgcataat
    31621 tgcctgggtt tacgggcgag ttcagggcgt aggatttcgc tacaccacac agtacgaagc
    31681 gaaaagactg ggcttaaccg ggtacgccaa aaatcttgac gatggcagcg tggaagtggt
    31741 tgcctgcggt gaagaagggc aggtagaaaa attaatgcag tggttaaaga gtggcggccc
    31801 gcgttctgcg cgtgtagaac gggtgctcag cgagccgcat catccctcgg gggaattaac
    31861 cgattttcgt atccgttaaa tacattttac cggttttggc aggccagcaa ttttggtggc
    31921 ttgcttagcc ggacctttcg gaaacagtcg gtataaatag cggctgttac ctttttcttc
    31981 gccaaattta ttcgccatcg cttttaccag catacgaatc gccggagaag tattgaattc
    32041 cagatagaaa tcacgcacaa aacgcaccac ttcccagtgt tctggcgaca gcgaaatccc
    32101 ttcgttctct gcaatcacca ctgccagcgg ctcactccac tggctgcttt ctttgagata
    32161 gccttcggta tccgtttcta tctctttacc ttcgaagatc agcataatta ttactacctt
    32221 aatcagactg ccggcagtgt aacaaaaaac aaagccccgc ataaagcgag gctatgaaag
    32281 tgttagcggg tgagattaat cgcggctggc gaagcccaga atgctcagca ggctgacgaa
    32341 gatgttgtac agcgaaacat acaggctaac cgtggcacga atatagttcg tttcaccgcc
    32401 atgaatgatg ttgctggttt caaacaaaat agcgccagag gagatcaaaa tgaagaccgc
    32461 gctgatcgcc agatgcagag caggcagctg caggaagata ttagcaacca taccaatcag
    32521 caccaccaca atacccgcca tcagcatacc gccgaggaac gacatatctt tgcgggtggt
    32581 cagcacatat gcagagcagc agaagaacac taacgccgtt ccgcccagtg ccatagcgat
    32641 tacgtcaccc attccggcag acagataggt attcagaatt ggtccgagga tataacccag
    32701 aaaaccggta aaggcgaatg cggagataat cccggtcggc ttattcgcca ttttataggt
    32761 caggaacatc aaaccataca tacccaccag cgtcagaatc agacccggag atggcagcat
    32821 cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc agcgtcaggc tcagcagaaa
    32881 ataggtatta cgcagcactt tatgggtgct aagcagtgat gtacggtcat gtgaagaact
    32941 aacaatacga tccattgagt cactctctat gacagatgta attaattaag cagcataatg
    33001 ataatgcgta agggcaccca gtagttttac ccatctttac gcatttgatc tggaacaggt
    33061 ttaacagcgg attatcaggt cattaagcaa atataacgcc ctgagaattt cggcaggcaa
    33121 aagaaaaagg ggttagcatt tagctaaccc cttatcttat ctggcggaag cgcagagatt
    33181 cgaactctgg aaccctttcg ggtcgccggt tttcaagacc gaagaaaaac aaataaaaat
    33241 cagtgtaata cgtgaaattg ctgaaaagtt aatcaaattt aacaccaaga aaaatcaagg
    33301 tattaggaag ttgctcatag ctagtatacc aagtcttttt gccagaaatt tttctggatt
    33361 gtctacttgc taaccacgag cggcaaatcc agtgttggtg tgatttttgt tttacgatcg
    33421 taaatcaaca cctgattttc tgttttatgc ccgctgaaaa tttgtttgtc gcgactgctg
    33481 ccttcgtaat ctgagatccc cttggctttt atgtcgtgaa aattgcaccc gaacggaacg
    33541 ccaacttttt gctcggctgc gcgtttagcc tgattccacc agttattcag cgttttagct
    33601 atgactttcc cgcctttggt tgtattgatc acatattcgc atgtacagga agatacattt
    33661 cgggctaact ggatcgctgt acgtaatcgc ggtgaccatt ccttgatttg tttggtgccg
    33721 gttttgtttt gctcaatgta gatcccttta tccataatat cctgccattt caactcaagc
    33781 acatcaccga gtcttgccgc acagagatag gaaatctcca ttgcaatgcg taactgtgga
    33841 attgcttccg catatatcgc cgcatactgt tcatcggtga tgtaaacagt gcgggcttta
    33901 agagagaatt ttctgactcc tttgcatggg ttattcttca catacccacg ctcatatccc
    33961 cacccgtata ctcgactcag gcttgccagt tcatgatttg cccaagccct cgtttatcca
    34021 tgaaaattct tacctgctca gttttgacat tatcagcaag cacttttccg aataccatca
    34081 gcaacgcctt ctgatgttgt cgataatctt tttgggttcg gggggccagt tctgtaaatg
    34141 caggggagtc cataaacatg tgccacaatt tagctacggt cattatgttg tggagttttg
    34201 ctttttccag ttcataattt tgccagactt tagctacgct ggttttccgc actcttccta
    34261 gccctatact tcttgtactt ccttcgggtt tccacacgta actgtaacca ttcgatctaa
    34321 cccgcggtgg cagtacatta tcttttttat tttttcttgg tcttcccatt gttcagcgcc
    34381 tcaaaatcgg gttcagcaga aaccagttca gatgtttttg gcatcgttgt cagaccgtgt
    34441 ggaatatccc tgcggagaac tatgggttcg tttttagggc cgattacaaa tgggatgccg
    34501 tgcagtctta actggtgttg ctgttttgtg tatcgctcgt atttcgtgat ctctttaatc
    34561 tctgctggag atagagttaa ttcgtacatg tggtcacgtg cctttacagc atgaccgccg
    34621 ccactatagc tggtgggcgg cgatatgggt tgaacatcaa aaatcagcct gactcgggag
    34681 cagtttttgc cagatggctg aaacgtattt cgcctggtaa cg
//
LOCUS       DABSYT020000042        34143 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.042, whole genome shotgun sequence.
ACCESSION   DABSYT020000042 DABSYT020000000
VERSION     DABSYT020000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.042"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            36..1001
                     /gene="iaaA"
                     /locus_tag="ID725_RS12230"
                     /old_locus_tag="pgaptmp_002446"
                     /pseudo
     CDS             36..1001
                     /gene="iaaA"
                     /locus_tag="ID725_RS12230"
                     /old_locus_tag="pgaptmp_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            988..2859
                     /gene="gsiA"
                     /locus_tag="ID725_RS12235"
                     /old_locus_tag="pgaptmp_002447"
     CDS             988..2859
                     /gene="gsiA"
                     /locus_tag="ID725_RS12235"
                     /old_locus_tag="pgaptmp_002447"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGV7851028.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            2879..4417
                     /gene="gsiB"
                     /locus_tag="ID725_RS12240"
                     /old_locus_tag="pgaptmp_002448"
     CDS             2879..4417
                     /gene="gsiB"
                     /locus_tag="ID725_RS12240"
                     /old_locus_tag="pgaptmp_002448"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGV7851029.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            4435..5355
                     /gene="gsiC"
                     /locus_tag="ID725_RS12245"
                     /old_locus_tag="pgaptmp_002449"
     CDS             4435..5355
                     /gene="gsiC"
                     /locus_tag="ID725_RS12245"
                     /old_locus_tag="pgaptmp_002449"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGV7851030.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            5358..6269
                     /gene="gsiD"
                     /locus_tag="ID725_RS12250"
                     /old_locus_tag="pgaptmp_002450"
     CDS             5358..6269
                     /gene="gsiD"
                     /locus_tag="ID725_RS12250"
                     /old_locus_tag="pgaptmp_002450"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGV7851031.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            6447..8795
                     /gene="pdeI"
                     /locus_tag="ID725_RS12255"
                     /old_locus_tag="pgaptmp_002451"
     CDS             6447..8795
                     /gene="pdeI"
                     /locus_tag="ID725_RS12255"
                     /old_locus_tag="pgaptmp_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGV7851032.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGDYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELINTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGLRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            8803..10131
                     /gene="dgcI"
                     /locus_tag="ID725_RS12260"
                     /old_locus_tag="pgaptmp_002452"
     CDS             8803..10131
                     /gene="dgcI"
                     /locus_tag="ID725_RS12260"
                     /old_locus_tag="pgaptmp_002452"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGV7851033.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(10201..10530)
                     /locus_tag="ID725_RS12265"
                     /old_locus_tag="pgaptmp_002453"
     CDS             complement(10201..10530)
                     /locus_tag="ID725_RS12265"
                     /old_locus_tag="pgaptmp_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7851034.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(10520..10906)
                     /locus_tag="ID725_RS12270"
                     /old_locus_tag="pgaptmp_002454"
     CDS             complement(10520..10906)
                     /locus_tag="ID725_RS12270"
                     /old_locus_tag="pgaptmp_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGV7851035.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGSKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            complement(11132..12457)
                     /gene="rimO"
                     /locus_tag="ID725_RS12275"
                     /old_locus_tag="pgaptmp_002455"
     CDS             complement(11132..12457)
                     /gene="rimO"
                     /locus_tag="ID725_RS12275"
                     /old_locus_tag="pgaptmp_002455"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGV7851036.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            12670..13053
                     /gene="bssR"
                     /locus_tag="ID725_RS12280"
                     /old_locus_tag="pgaptmp_002456"
     CDS             12670..13053
                     /gene="bssR"
                     /locus_tag="ID725_RS12280"
                     /old_locus_tag="pgaptmp_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGV7851037.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            13164..14279
                     /gene="yliI"
                     /locus_tag="ID725_RS12285"
                     /old_locus_tag="pgaptmp_002457"
     CDS             13164..14279
                     /gene="yliI"
                     /locus_tag="ID725_RS12285"
                     /old_locus_tag="pgaptmp_002457"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGV7851038.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(14276..14902)
                     /gene="gstB"
                     /locus_tag="ID725_RS12290"
                     /old_locus_tag="pgaptmp_002458"
     CDS             complement(14276..14902)
                     /gene="gstB"
                     /locus_tag="ID725_RS12290"
                     /old_locus_tag="pgaptmp_002458"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGV7851039.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            15148..16350
                     /gene="dacC"
                     /locus_tag="ID725_RS12295"
                     /old_locus_tag="pgaptmp_002459"
     CDS             15148..16350
                     /gene="dacC"
                     /locus_tag="ID725_RS12295"
                     /old_locus_tag="pgaptmp_002459"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGV7851040.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(16397..17155)
                     /gene="deoR"
                     /locus_tag="ID725_RS12300"
                     /old_locus_tag="pgaptmp_002460"
     CDS             complement(16397..17155)
                     /gene="deoR"
                     /locus_tag="ID725_RS12300"
                     /old_locus_tag="pgaptmp_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGV7851041.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(17213..17809)
                     /gene="ybjG"
                     /locus_tag="ID725_RS12305"
                     /old_locus_tag="pgaptmp_002461"
     CDS             complement(17213..17809)
                     /gene="ybjG"
                     /locus_tag="ID725_RS12305"
                     /old_locus_tag="pgaptmp_002461"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGV7851042.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            18094..19326
                     /gene="mdfA"
                     /locus_tag="ID725_RS12310"
                     /old_locus_tag="pgaptmp_002462"
     CDS             18094..19326
                     /gene="mdfA"
                     /locus_tag="ID725_RS12310"
                     /old_locus_tag="pgaptmp_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGV7851043.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(19367..19651)
                     /gene="ybjH"
                     /locus_tag="ID725_RS12315"
                     /old_locus_tag="pgaptmp_002463"
     CDS             complement(19367..19651)
                     /gene="ybjH"
                     /locus_tag="ID725_RS12315"
                     /old_locus_tag="pgaptmp_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGV7851044.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(19737..20552)
                     /gene="ybjI"
                     /locus_tag="ID725_RS12320"
                     /old_locus_tag="pgaptmp_002464"
     CDS             complement(19737..20552)
                     /gene="ybjI"
                     /locus_tag="ID725_RS12320"
                     /old_locus_tag="pgaptmp_002464"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGV7851045.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(20552..21760)
                     /gene="ybjJ"
                     /locus_tag="ID725_RS12325"
                     /old_locus_tag="pgaptmp_002465"
     CDS             complement(20552..21760)
                     /gene="ybjJ"
                     /locus_tag="ID725_RS12325"
                     /old_locus_tag="pgaptmp_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7851046.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            21844..22380
                     /gene="rcdA"
                     /locus_tag="ID725_RS12330"
                     /old_locus_tag="pgaptmp_002466"
     CDS             21844..22380
                     /gene="rcdA"
                     /locus_tag="ID725_RS12330"
                     /old_locus_tag="pgaptmp_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGV7851047.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(22555..24240)
                     /gene="ybjL"
                     /locus_tag="ID725_RS12335"
                     /old_locus_tag="pgaptmp_002467"
     CDS             complement(22555..24240)
                     /gene="ybjL"
                     /locus_tag="ID725_RS12335"
                     /old_locus_tag="pgaptmp_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGV7851048.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            24510..24887
                     /gene="ybjM"
                     /locus_tag="ID725_RS12340"
                     /old_locus_tag="pgaptmp_002468"
     CDS             24510..24887
                     /gene="ybjM"
                     /locus_tag="ID725_RS12340"
                     /old_locus_tag="pgaptmp_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGV7851049.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(24917..25174)
                     /gene="grxA"
                     /locus_tag="ID725_RS12345"
                     /old_locus_tag="pgaptmp_002469"
     CDS             complement(24917..25174)
                     /gene="grxA"
                     /locus_tag="ID725_RS12345"
                     /old_locus_tag="pgaptmp_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGV7851050.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            25334..25621
                     /gene="ybjC"
                     /locus_tag="ID725_RS12350"
                     /old_locus_tag="pgaptmp_002470"
     CDS             25334..25621
                     /gene="ybjC"
                     /locus_tag="ID725_RS12350"
                     /old_locus_tag="pgaptmp_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGV7851051.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            25605..26327
                     /gene="nfsA"
                     /locus_tag="ID725_RS12355"
                     /old_locus_tag="pgaptmp_002471"
     CDS             25605..26327
                     /gene="nfsA"
                     /locus_tag="ID725_RS12355"
                     /old_locus_tag="pgaptmp_002471"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGV7851052.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            26388..27290
                     /gene="rimK"
                     /locus_tag="ID725_RS12360"
                     /old_locus_tag="pgaptmp_002472"
     CDS             26388..27290
                     /gene="rimK"
                     /locus_tag="ID725_RS12360"
                     /old_locus_tag="pgaptmp_002472"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGV7851053.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            27378..27854
                     /gene="ybjN"
                     /locus_tag="ID725_RS12365"
                     /old_locus_tag="pgaptmp_002473"
     CDS             27378..27854
                     /gene="ybjN"
                     /locus_tag="ID725_RS12365"
                     /old_locus_tag="pgaptmp_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGV7851054.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            28205..29317
                     /gene="potF"
                     /locus_tag="ID725_RS12370"
                     /old_locus_tag="pgaptmp_002474"
     CDS             28205..29317
                     /gene="potF"
                     /locus_tag="ID725_RS12370"
                     /old_locus_tag="pgaptmp_002474"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGV7851055.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            29412..30545
                     /gene="potG"
                     /locus_tag="ID725_RS12375"
                     /old_locus_tag="pgaptmp_002475"
     CDS             29412..30545
                     /gene="potG"
                     /locus_tag="ID725_RS12375"
                     /old_locus_tag="pgaptmp_002475"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGV7851056.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            30555..31508
                     /gene="potH"
                     /locus_tag="ID725_RS12380"
                     /old_locus_tag="pgaptmp_002476"
     CDS             30555..31508
                     /gene="potH"
                     /locus_tag="ID725_RS12380"
                     /old_locus_tag="pgaptmp_002476"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGV7851057.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            31505..32350
                     /gene="potI"
                     /locus_tag="ID725_RS12385"
                     /old_locus_tag="pgaptmp_002477"
     CDS             31505..32350
                     /gene="potI"
                     /locus_tag="ID725_RS12385"
                     /old_locus_tag="pgaptmp_002477"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGV7851058.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            32410..32898
                     /gene="ybjO"
                     /locus_tag="ID725_RS12390"
                     /old_locus_tag="pgaptmp_002478"
     CDS             32410..32898
                     /gene="ybjO"
                     /locus_tag="ID725_RS12390"
                     /old_locus_tag="pgaptmp_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGV7851059.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            32939..34066
                     /gene="rlmC"
                     /locus_tag="ID725_RS12395"
                     /old_locus_tag="pgaptmp_002479"
     CDS             32939..34066
                     /gene="rlmC"
                     /locus_tag="ID725_RS12395"
                     /old_locus_tag="pgaptmp_002479"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGV7851060.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
BASE COUNT         8295 a         8166 c         8979 g         8703 t
ORIGIN      
        1 caaaagtggc gagccaccct taatggacga atactatggg caaagcagtc attgcaattc
       61 atggtggcgc aggtgcaatt agccgcgcgc agatgagtct gcaacaggaa ttacgctaca
      121 tcgaggcgtt gtctgccatt gttgaaaccg ggcagaaaat gctggaagcg ggcgaaagtg
      181 cgctggatgt ggtgacggaa gcggtgcgtc tgctggaaga gtgtccactg tttaacgccg
      241 gaattggcgc cgtctttacg cgtgatgaaa cgcatgaact ggacgcctgt gtgatggatg
      301 gtaacaccct gaaagccggt gcggtggcgg gcgttagtca tctgcgtaat ccggttcttg
      361 ccgcccggct ggtgatggag taaagcccgc atgtgatgat gattggcgaa ggggcagaaa
      421 attttgcgtt tgctcatggc atggagcgcg tctcaccgga gattttctcc acgcctttgc
      481 gttatgaaca actaatggca gcgcgcgagg aaggggcaac agtcctcgac catagcggtg
      541 cgccactgga tgaaaaacag aaaatgggca ccgtgggggc cgtggcgttg gatttagacg
      601 gcaatctggc ggcagccacg tccacgggcg gaatgaccaa taaattaccc ggacgagttg
      661 gcgatagccc cttagtgggt gccggatgct acgccaataa cgccagtgtg gcggtttctt
      721 gtaccggcac gggcgaagtc ttcatccgcg cgctggcggc atatgacatc gccgcgttaa
      781 tggattacgg cggattaagt ctcgcggaag cctgcgagcg ggtagtaatg gaaaaactcc
      841 ctgcgcttgg cggtagcggt ggcttaatcg ctatcgacca tgaagggaat gtcgcgctac
      901 cgtttaacac cgaaggaatg tatcgcgcct ggggctacgc aggcgatacg ccaaccaccg
      961 gtatctaccg tgaaaaaggg gacaccgttg ccacacagtg atgaacttga tgccggtaat
     1021 gtgctggcgg ttgaaaatct taatattgcc tttatgcagg accagcagaa aatagctgcg
     1081 gtccgcaatc tctcttttag tctgcaacgc ggtgagacgc tggcaattgt tggcgaatcc
     1141 ggctccggta agtcagtgac tgcgctggca ttgatgcgtc tgttggaaca ggcgggcggt
     1201 ttagtgcagt gcgataaaat gctgttgcgg cggcgcagtc gtgatgtgat tgaacttagc
     1261 gagcagagcg ctgcacaaat gcgccatgtg cgcggtgcgg atatggcgat gatatttcag
     1321 gagccgatga catcgctgaa cccagtattt actgtgggtg aacagattgc cgaatcaatt
     1381 cgtctgcatc agaacgccag tcgtgaagaa gcgatggtcg aggcgaagcg gatgctggat
     1441 caggtacgca ttccggaggc acaaaccatt ctttcacgtt atccgcatca actctctggc
     1501 gggatgcgcc agcgagtgat gattgcgatg gcgctgtcat gccgcccggc agtgctgata
     1561 gccgatgagc caaccaccgc gctggatgtc actattcagg cgcagatcct gcaattaatc
     1621 aaagtattgc aaaaagagat gtcgatgggc gttatcttta tcactcacga tatgggcgtg
     1681 gtggcagaga ttgccgatcg ggtactggtg atgtatcagg gcgaggcggt ggaaacgggt
     1741 accgtcgaac agatttttca tgcaccgcaa catccctaca cccgtgcgct gttagctgct
     1801 gttccgcaac ttggtgcgat gaaagggtta gattatcccc gacgtttccc attgatatcg
     1861 cttgaacatc cagcgaaaca ggcccccccc atcgagcaga aaacggtggt ggatggcgaa
     1921 cctgttttac gggtgcgtaa tctggtcacc cgtttccctt tgcgcagcgg tttgttgaat
     1981 cgcgtaacgc gggaagtgca tgccgttgag aaagtcagtt ttgatctctg gcctggcgaa
     2041 acgctatcgc tggtgggcga gtctggcagc ggtaaatcca ctaccgggcg ggcgttgctg
     2101 cgcctggtcg aatcgcaggg cggcgaaatt atctttaacg gtcagcgaat cgataccttg
     2161 tcacccggca aacttcaggc attgcgccgc gatattcagt ttatttttca ggacccttac
     2221 gcttcgctgg acccacgtca gaccatcggt gattcgatta tcgaaccgct gcgcgtacac
     2281 ggtttattgc caggtaaaga agcggttgca cgcgttgcgt ggttgctgga gcgcgtgggc
     2341 ctgttacctg aacatgcctg gcgttacccg catgagtttt ccggcggtca gcgccagcgc
     2401 atctgcattg ctcgcgcgtt ggcattgaat ccaaaagtga tcattgccga cgaagccgtt
     2461 tcggcgctgg atgtttcaat tcgcgggcag attatcaact tgttgctcga tctccagcgt
     2521 gatttcggca ttgcgtatct gtttatctcc cacgatatgg ctgtggtaga gcggattagt
     2581 catcgtgtgg cggtgatgta tctcgggcaa attgttgaaa ttggtccacg gcgcgcggtc
     2641 ttcgaaaacc cgcagcatcc ttatacgcgt aaattactgg cggcagttcc ggtcgctgaa
     2701 ccgtcccgac aacgaccgca gcgtgtactg ctgtcggacg atcttcccag caatattcat
     2761 ctgcgtggcg aagaggtggc agccgtctcg ttgcaatgcg tcgggccggg gcattacgtc
     2821 gcacaaccac aatcagaata cgcattcatg cgtagataac attcaggcgg agaataaaat
     2881 ggcaagagct gtacaccgta gtgggttagt ggcgctgggc attgcgacag cgttgatggc
     2941 atcttgtgca ttcgctgcca aagatgtggt ggtggcggta ggatcgaatt tcaccacgct
     3001 cgatccgtat gacgcgaatg acacgttatc tcaggccgta gcgaaatcgt tttaccaggg
     3061 gctgttcggt ctggataaag agatgaaact gaaaaacgtg ctggcggaga gttataccgt
     3121 ttccgatgac ggcattactt acaccgtgaa attgcgggaa ggaattaaat tccaggatgg
     3181 caccgatttc aacgcagcgg cggtaaaagc gaatctggac cgggccagcg atccggcgaa
     3241 tcatcttaaa cgctataacc tgtataagaa tattgctaaa acggaagcga tcgatccgac
     3301 aacggtaaag attaccctca aacagccgtt ctcagcgttt attaatattc ttgcccatcc
     3361 ggcgaccgcg atgatttcac cggcagcgct ggaaaaatat ggcaaggaga ttggttttca
     3421 tccggtggga accggaccgt atgaactgga tacctggaat cagaccgatt ttgtgaaggt
     3481 gaaaaaattc gcgggttact ggcagccagg attgcccaaa ctggacagca taacctggcg
     3541 tccggtggcg gataacaaca cccgcgcggc aatgctgcaa accggtgaag cgcagtttgc
     3601 tttccccatt ccttacgagc aggccgcact gctggagaaa aacaaaaata tcgagttgat
     3661 ggccagtccg tcaattatgc agcgttatat cagtatgaac gtgacgcaaa agccattcga
     3721 taacccgaag gtccgtgagg cgctgaatta cgccattaac cgtccggcgc tggtgaaagt
     3781 ggccttcgcg ggctatgcaa cgccagctac tggtgtggta ccgccgagta tcgcctacgc
     3841 gcaaagttat aaaccgtggc cttacgatcc agtgaaagcg cgcgaattac tgaaagaggc
     3901 gggatatccc aacggtttca gtaccacgct gtggtcgtca cataaccaca gcaccgcgca
     3961 gaaagtgctg caatttaccc agcagcagtt agcgcaggtc gggattaaag cccaggtgac
     4021 tgcgatggat gccggacagc gggcggcaga agttgaaggt aaagggcaaa aagagagcgg
     4081 cgtgcggatg ttctacactg gctggtcggc ttcaaccggc gaagcggact gggcactatc
     4141 gccgttgttt gcctcgcaaa actggccacc gacgctgttt aataccgcgt tttacagcaa
     4201 taaacaggtg gatgacttcc tggctcaggc actgaaaact aatgatccgg cggaaaagac
     4261 ccgcttatat aaggcggcgc aggatatcat ctggcaagag tcgccgtgga tcccgctggt
     4321 ggtagaaaaa ctggtgtcgg cacacagtaa aaacctgacc ggtttttgga tcatgccaga
     4381 taccggcttc agctttgaag acgcggattt gcaataagca acgcagggag tggaatgctt
     4441 aattacgtta tcaaacgatt actggggttg attccgacgc tgtttatcgt ctcggtgctg
     4501 gtgtttttat ttgtgcatat gctgcccggc gatccggcgc gattgattgc cgggcccgaa
     4561 gctgatgcgc aggttataga actggtgcgt cagcagctgg ggttggatca gccgctgtat
     4621 caccagttct ggcactatat cagcaatgct gtgcaggggg attttggcct gtcgatggtg
     4681 tcacgtcgtc cggttgccga tgagattgcc aggcgcttta tgccaacgct gtggctgacc
     4741 ataaccagta tggtctgggc ggtgatattt ggtatggcgg cgggaattat cgccgccgtc
     4801 tggcgtaacc gttggccgga tcgattgagt atgaccattg cggtgtcggg gatctcgttt
     4861 ccggcatttg cgctggggat gcttttaatt caggtattct ccgttgaact gggctggctg
     4921 cctaccgtgg gagcagacag ttggcagcac tacattttac cctccctgac gctcggcgcg
     4981 gcagtggccg ccgtgatggc gcgctttacc cgcgcgtcgt ttgtcgacgt tttaagcgaa
     5041 gattatatgc gtaccgcgag ggcgaaaggg gtgagcgaaa cctgggttgt cctcaaacac
     5101 gggctacgca acgcgatgat cccggtagtg accatgatgg gcttacagtt tggctttttg
     5161 ctcggtggtt ccatcgtggt ggagaaagtt ttcaactggc ccggacttgg acgcttactc
     5221 gttgactccg tagaaatgcg cgattacccg gtgattcagg cggaaattct gcttttctcg
     5281 ctggaattta ttcttatcaa cttagtggtg gatgtgcttt acgccgccat taacccggct
     5341 atcaggtaca agtaaggatg cgactattta actggcgacg tcaggcggtg ttaaacgcca
     5401 tgccactggt caaacctgac caggtacgta caccgtggca tgaattctgg cgacgatttc
     5461 gccgtcagca tatggcgatg accgccgcat tattcgttat tttattgatt gtggtggcca
     5521 tttttgcacg ctggatcgct ccctatgacg ccgaaaatta ttttgattat gacaatctga
     5581 ataacggacc ttctttgcag cactggtttg gcgtcgattc actggggcgc gacattttca
     5641 gccgtgtcct ggttggtgcg caaatctcgc tggcggcggg cgtgtttgcc gtgtttatcg
     5701 gtgcggcgat agggacgttg ctgggcttgt tggctggata ttatgaaggc tggtgggatc
     5761 ggctgatcat gcgcatttgc gatgtgctgt ttgccttccc gggtatttta ctggcgatcg
     5821 ctgttgttgc ggtgttggga agcggcattg ctaacgtgat tattgcagtc gccatttttt
     5881 ccatccctgc gtttgcccgc ctggtgcgcg gcaacacgct ggtgttgaaa cagcaaacct
     5941 ttattgagtc agcacgcagt attggtgcca gcgatatgac cattttgttg cgtcatatcc
     6001 tgcctgggac cgtctcttct atcgtggtgt ttttcaccat gcgcattggt acctcgatta
     6061 tctctgccgc cagcctgtca tttctcggcc tcggtgcgca gccgccgaca ccagagtggg
     6121 gagcaatgct caatgaggct cgagcggata tggttatcgc gccgcatgtc gctgtttttc
     6181 cggccctggc tatttttctg actgtactgg cgttcaattt gttgggcgat ggtttacgcg
     6241 atgcgctgga tccgaaaatt aaaggatagt tacgtttgaa tattgcttga aagagtaatc
     6301 acctcacagg aaattattgc cctaagcaag tgttgtaact ttctgctgat tttgtagaat
     6361 cgggtaattt ggttaaatag ccgcagcaag ggactgtttt tgcagcggca cagcgttcag
     6421 acagttattt tgttaaatgt attaacatgc tgagtttata cgaaaagata aagataaggc
     6481 tgataatttt atttttattg gcagcactgt catttattgg cctttttttc atcattaact
     6541 atcaactggt atcggaacgc gcggtaaaac gtgccgatag ccgctttgaa cttattcaga
     6601 aaaacgttgg ctatttcttt aaagatattg aacgttcggc cctgacatta aaggactcac
     6661 tgtatttatt aaaaaataca gaggagattc aacgcgctgt gatcctgaaa atggaaatga
     6721 tgccattttt agactcggtg ggactggtac ttgatgataa taaatattat ctcttttcgc
     6781 ggagggcgaa cgataaaatc gttgtttatc atcaggaaca agtaaatgga ccgcttgtcg
     6841 acgagtcagg gcaggttatt tttgccgatt ttaacccatc gaaacgaccg tggtcggtgg
     6901 cttcagatga ctctaacaac agctggaatc cggcctacaa ttgctttgat cgtccgggta
     6961 aaaaatgtat ctcttttacg ctacgcatca acggcaaaga tcacgatttg ttagcggtgg
     7021 ataaaattca tgtcgattta aactggcgat atctgaacga gtatcttgat caaatcagcg
     7081 ctaatgatga agttctattt ttgaaacaag gccatgagat cattgccaag aatcaactcg
     7141 ctcgtgaaaa actgattatt tataatagcg aaggtgatta taatattatt gattctgtcg
     7201 atactgaata tatcgaaaaa acatcagcgg tgccaaataa cgcattattc gaaatctatt
     7261 tttattatcc gggcggtaat ttattgaacg catcagataa acttttttat ctgccgtttg
     7321 cgttcattat tatcgtattg ctggtggttt atttaatgac cactcgtgtg ttccgtcggc
     7381 aattttctga aatgacagag ctgattaata cgctggcgtt tttgcctgac tcaacggatc
     7441 agatcgaggc tctgaaaatt cgcgaaggcg atgcgaaaga gattatcagc atcaaaaatt
     7501 cgatcgcgga aatgaaagat gccgaaattg aacggtcaaa taaattgctc tcactgatct
     7561 cttacgatca ggaaagtggt tttattaaaa atatggcgat tattgagtcc aacaataatc
     7621 agtatctggc tgtggggatc atcaaactgt gtggtctgga agccgtggaa gctgtgtttg
     7681 gtgttgatga acgcaataaa atcgtcagga aattgtgtca gcgaattgct gagaaatatg
     7741 cacaatgctg cgatatcgtg acattcaatg ccgatctcta tttacttctg tgtcgggaaa
     7801 atgtacagac atttacccgt aaaatagcga tggtaaacga ttttgacagc agctttggct
     7861 accgcaatct gcgcatccat aagtctgcca tttgtgaacc tttgcagggg gaaaacgcct
     7921 ggagttacgc agaaaaactg aaactggcga tttccagtat ccgtgaccat atgttctccg
     7981 agtttatttt ctgtgatgac gcgaaactca acgaaataga agagaatatc tggattgcgc
     8041 gtaatattcg ccatgcaatg gaaattggcg aactattcct cgtctatcaa ccgatcgttg
     8101 atattaacac ccgcgccatt ctgggcgcgg aggcgttgtg ccgttgggtg tctgcggagc
     8161 gggggatcat ttcaccgctg aagttcatta ccattgctga agatatcggg tttatcaatg
     8221 agctgggtta tcagattatt aaaacggcaa tgggtgaatt cagacatttt agtcagcgtg
     8281 catcgctgaa ggatgatttc ttactgcata ttaatgtttc gccctggcag ttaaacgaac
     8341 cgcactttca tgagcgtttt accaccatca tgaaagaaaa tggcctgaag gcgaacagcc
     8401 tctgtgttga gatcactgaa accgtgatcg agcgaattaa tgaacatttt tatctcaata
     8461 ttgaacaact gcgtaaacaa gggttacgga tatcgattga tgactttggc accggtttgt
     8521 caaacctgaa acgtttttat gaaattaatc cagacagcat aaaggtggac tcgcaattta
     8581 ccggcgatat tttcggtact gcgggaaaaa ttgtgcgcat tattttcgac ctggcacgct
     8641 ataaccggat cccggtgatt gcggaaggcg tagagagcga agacgttgcg cgcgaattaa
     8701 tcaaattagg atgtgttcag gctcaggggt atctgtatca gaaacccatg ccattctccg
     8761 cctgggataa aagtggaaaa ttagtaaaag agtagtttac gtatgtccag aatcaataag
     8821 ttcgtactta cagtcagtct gctgattttt atcatgattt cagcagttgc ctgcgggatc
     8881 tacactcaaa tggtaaagga acgggtgtat agcctgaaac agtccgttat tgatactgct
     8941 tttgcggtgg caaatattgc tgaatatcgg cgtagcgtgg caattgatct tatcaacacg
     9001 ctaaatccca cggaggaaca gctgttggtt ggcttacgca cagcttacgc cgactcggtt
     9061 tcaccctctt atttgtacga tgtcggtcct tatctgattt ccagtgacga atgtattcag
     9121 gtaaaggagt tcgagaaaaa ttattgtgca gatattatgc aggttgtgaa gtatcgacat
     9181 gtcaaaaata cagggtttat ctcttttgac ggtaaaacct tcgtctatta cctctatccg
     9241 gtaactcaca atcgtagtct gatatttttg cttggtctgg agcgtttttc tttactgtca
     9301 aaatcgctgg cgatggacag cgagaacctg atgttctctc tgtttaagaa cggtaaaccg
     9361 gtgaccggtg atgaatataa tgctaaaaac gccatcttca ccgtttcgga agcgatggag
     9421 cacttcgcct atttgccgac cggattgtac gtatttgcgt ataaaaaaga tgtttatttg
     9481 cgggtctgta cattgattat tttctttgcc gcattggtgg cagtgatatc gggtgccagt
     9541 tgcctctatc tggtacgcag agtgattaat cgtggtattg tggagaaaga agccattatt
     9601 aataaccatt ttgaacgcgt actggatggc gggcttttct tttcggctgc cgatgtcaaa
     9661 aaactctaca gtatgtataa ctcggcgttc ctggacgacc tgaccaaagc aatgggtaga
     9721 aaatcctttg acgaagattt aaaagcgctg ccggaaaaag gcggttattt gtgcctgttt
     9781 gacgtcgata aattcaaaaa tattaacgac accttcggtc atttgctggg cgatgaagtg
     9841 ttgatgaaag tggtgaaaat ccttaaatca cagatcccgg tagataaagg taaagtctac
     9901 cgcttcggcg gtgacgaatt tgcggtgatt tatactggcg gcacgctgga agagttacta
     9961 tcgattctaa aagaaatcgt tcatttccag gtgggaagca ttaatttaag caccagtatc
    10021 ggtgtagcgc attcaaatga atgtactacc gtcgaacgct tgaaaatgct ggcggatgag
    10081 cggctgtata agagtaagaa aaacggcagg gctcagatta gctggcagta gaattagaat
    10141 agtgttagta cccctgaatc aaggggtact aatgaatgtg gtaaatcatt tgcaaacatt
    10201 ttactttgca aaaacttccg gcccttttat tgccaatgta ttcaaaatac gtagtgccgg
    10261 gccgctcggt tttatttcat tacgttccca tttcgagaca ctttcttttg acattcccat
    10321 tgtatgtgcc agcacggatt gtgacatgcc gtatcgggtg cggatggctt tgatctcttc
    10381 acccgtcatt gatttgactt tggctatacg ggcgctgact tcacgggctt tgaccatgac
    10441 ggcaatggca tcaacctcac tatcagacat caggcccagt gcattcagtt cctttgcatc
    10501 ttccagcatc atttgtaatt tatcgctcat ttttcacctc aatcaagaag ccagtttcga
    10561 tcaaatcctc tatttgctta ttgctaaagg ccagaaatgc atttgccatt tttttgtagg
    10621 ctgccagttc atcatcttcg atttctttgc ttcctttact gcttagacca ctcttggacc
    10681 aaccatcata aaaaaacaca tgatttgctg atttaaaaaa gataattgtt ctggctccac
    10741 cactctttcc ttgttggagg ggtaaccgtt ttttgatgac accgctacct aaatcagcct
    10801 cccatatgcc ttgcatcact tcttcagcgg cagccatgag aactttatcg tgaataccaa
    10861 gttttttcgt gctttggctg aatcgccttg ccttatacac acccatacat ccatccttgt
    10921 ggtaatgttt actatagtaa ccattacgct tatttcaagt aattgcgatc aaaactttaa
    10981 tttggtgtga aaaagtgaga gttgcctcga gtacgattca tttttctcga tttatgcgtc
    11041 aggggatttt gtgcgtaagg gtaggacatc gggaagggta gtagaggcag atcgaagaac
    11101 aatatccttg ttagatctgc ctttagatta attaaacccg gctaccccac aaatcgtact
    11161 catcggcgtg ctcgactttc acacgcagga tatcacccgg cttaacgttg gtttcaccat
    11221 tgagataaac cgcaccgtcg atttccggtg catctgccat gctgcgacca atcgcgcctt
    11281 cttcgtccac ttcgtcgata atcaccagaa tttcacggcc cactttctct tgcaggcgct
    11341 cggcggaaat ctgctgctgc aactgcatga aacggttcca gcgttcttct ttcacttctt
    11401 ccggaacctg gtcaggcagg gcattggcgt ctgcaccttc aaccgggctg tatttaaagc
    11461 agccaacgcg atccagacgc gcttctttca ggaagtcgag tagcatctgg aaatcttctt
    11521 ccgtctcgcc agggaagccg acaataaagg ttgagcgtag ggtcagttcc gggcagattt
    11581 cgcgccactg tttgatgcgc gccagttggc gatctacaga acccggacgc ttcatcagtt
    11641 tgagaatgcg cgggctggcg tgctgcaacg gaatgtccag atacggcagg attttgcctt
    11701 ctgccatcag tgggatgacg tcgtccacat gcggataagg gtaaacgtag tgcagacgtg
    11761 tccagatccc cagtttcgat aactgttcgc acaggctgac catgctggtt tttaccggct
    11821 cgccgttgtg gaagccagta cgatgtttaa catcaacgcc ataggcggaa gtatcctgcg
    11881 agatcaccag aatctcttta acgcccgcat ctaccagacg tttcgcttca cttaatactt
    11941 cgccaatcgg acggctcacc aggtcgccgc gcatagacgg aataatgcag aaggtgcagc
    12001 ggtgattaca gccttcagaa attttcagat aggcataatg acgcggcgtc agtttgacac
    12061 cttgttctgg caccaggctc aggaatgggt tgtgtttcgg ttttggcacg tagtgatgaa
    12121 cgtgctccag aacctgctca tagctatgag gcccggtgat ttccagcact ttcgggtgga
    12181 cttcgcggat ctgatcttct tttgccccca gacaaccggt cacaattacc ttgccgtttt
    12241 cattcaacgc ttcaccaatg gcttccagtg attcttgtac cgcgctgtca ataaagccgc
    12301 aggtgttgac gatcaccatg tccgcatcgt catagctcgg taccacgtca taaccttcag
    12361 tgcggagttc ggtgagaata cgctctgaat cgacaaggtt tttcggacag ccaagggaaa
    12421 caaagccgat tttcggctgg ggagttactt tgctcatagc ttaaaaaata ttcagttaca
    12481 ggaaaggtca gggcagggat tctacagagt tctggataaa atttgtatcg caatctcatt
    12541 cgctggcgga ggcgaaggaa atgtaaattt tgttaattcg gcgtgaagaa ttgatcctgg
    12601 acagcattat gctcaaaaaa tagccatact atttaattgc aacaaggctg gaaagaggag
    12661 gatcgaagta tgttcgttga cagacagcga atcgatctgc tgaaccggtt gatcgacgca
    12721 cgcgttgacc tcgccgcata cgtgcaactg aggaaggcaa aaggatacat gtccgtcagc
    12781 gaaagcaatc atctacgaga taactttttt aaactgaatc gcgaactgca cgataaatcg
    12841 ctgcggttga atcttcatct ggatcaggaa gagtggagtg ctcttcatca tgctgaagaa
    12901 gcattagcga cagccgcagt atgtttgatg agtgggcacc atgattgccc gactgttatt
    12961 accgtcaacg ccgataagct tgaaaattgt ctgatgagct taacgctgag tatccagagc
    13021 ctgcagaagc acgccatgct tgagaaggcc tgaaaactaa gggggagaaa gcgtctcccc
    13081 cttcatgttt aagtttttgt aaaaatgaat ttgttatctc ctccactgac tacgctttaa
    13141 gccagagtca atccggaggc gttatgcatc gacaattctt tttccttgtg ccccttattt
    13201 gtctttcttc cgctctctgg gcggctcctg caacggtaaa tgtcgaagta ctgcaagaca
    13261 aactcgacca tccctgggca ctggcctttt tacccgataa ccacggtatg ttaatcactc
    13321 tgcgcggcgg cgagttgcgt cactggcaag caggaaaagg attatctgcg ccgctttccg
    13381 gagttccgga cgtttgggcg cacgggcagg gcggcctgct ggacgtggtt ttagcgcctg
    13441 attttgctca gtctcgccgc atctggttaa gttattccga agttggcgat gatggcaaag
    13501 ccggaactgc tgtggggtat ggccgcttaa gtgatgatct ctcaaaagtg accgactttc
    13561 gcaccgtctt tcgccagatg ccaaaactgt ctaccggcaa ccattttggc gggcggctgg
    13621 tattcgacgg taaaggttat ctttttattg ctctgggcga aaacaatcag cgcccgacgg
    13681 cgcaggatct ggataaatta cagggcaaac tggtgcgtct gaccgaccag ggcgaaatcc
    13741 cggatgataa tccttttata aaggaatccg gtgcgcgcgc cgagatctgg tcttatggca
    13801 ttcgtaatcc gcaaggaatg gcgatgaatc cgtggagtaa tgcactgtgg ctgaatgaac
    13861 atggcccgcg cggtggtgat gaaattaata tcccgcaaaa aggcaaaaac tacggctggc
    13921 cgctggcaac ctggggaatc aactattcag gctttaagat accggaagcg aaaggggaga
    13981 tcgtcgccgg gaccgagcaa cctgtttttt actggaaaga ttcgcccgcc gtgagcggca
    14041 tggccttcta taacagcgat aaattccccc agtggcagca aaaattattt attggcgcgc
    14101 tgaaagataa agatgtcatt gtgatgagcg tcaacggcga caaagtaaca gaagatggcc
    14161 gtattttaac ggacagaggg cagcgaattc gtgatgttcg cactggaccc gacggttatt
    14221 tatacgttct caccgacgag tccagtgggg aattacttaa agttagccca cgcaattagc
    14281 taacgggaat catcaccact ttgcgcactg cggggcgttc agtgagttgc tgataccagc
    14341 gttgcagatt tggacgcggt gtccaggtca ggccaacatt aaacaaattg tagatgaacg
    14401 gtgcgatagc gatatcgccc acaccaaact cgtcgccaga gaaccatttt acttttgcca
    14461 gttccgcatc gagcagggca aacagggcgt cgcactcttt gcaactggca tcaatggcgg
    14521 cctgatcgcg ctcttccggt ggtgttctga ctaatcccat caggatcccg cgatgagcat
    14581 tactgagtgt ctggtttgcc cagtccatcc atttttctgc ttccgcacga cgtgccggtg
    14641 agtcgatcca caggcgtttt tgcccgtact gtgccgccag atagcggaca atggcgtttg
    14701 attcccaaag aatgagatca ctttcgtcgt cacgcaacaa cggcaccagc ccgttagggt
    14761 tcatcgccag aaaatcagca tcgtgattta tcccaaactc acggcccgcg agaatttgct
    14821 cataaggtag ttccagttct tcgagcgtca gcaatacttt ttttacgttg gttgaattat
    14881 tccgacccca cagcgtaatc atactcaccc cttttccgac aggcagccac aggctgaaca
    14941 ggactccgat ggtgaagtaa agttaacttt taagcaacag ctggcaaaaa aatgacacca
    15001 gaagcagcga agcggcagga tattgcataa actttaaaaa ctttaccaac ttacggtttc
    15061 tttaagtttg tgtgtgcgtt attaatcacc gaacttatca tacggcgata taacgtattt
    15121 ttttgaatgg atactcgggt ggcatttatg acgcaatact cctctctcct tcgtggtctt
    15181 gcagcgggtt ctgcattttt attccttttt gccccaacgg cattcgcggc cgaacaaacc
    15241 gttgaagcgc cgagcgtgga tgcgcgtgca tggattttaa tggattacgc cagcggtaaa
    15301 gtgctggcag aaggcaatgc ggatgagaaa ctggatcccg cgagcctgac taaaatcatg
    15361 accagctatg tggttgggca ggcgcttaag gccgataaga ttaaactcac cgatatggtg
    15421 acggtcggta aagatgcctg ggcgacggga aatccggcac tgcgtggttc atcggtaatg
    15481 ttcctcaaac cgggcgatca ggtttcggtg gcagacttga acaaaggtgt gattatccag
    15541 tccggtaatg acgcctgtat tgcgctggcc gattacgttg ccgggagcca ggagtcattt
    15601 attggtttga tgaatggtta tgccaaaaaa ctggggctga ccaacactac cttccagacg
    15661 gtgcacggtc tggatgcgcc ggggcagttc agtaccgcgc gcgatatggc attgctgggt
    15721 aaagcattga tccacgatgt gccggaagag tacgccattc ataaagagaa agagttcacc
    15781 ttcaacaaaa ttcgtcagcc taaccgtaac cgtctgctgt ggagcagcaa tctgaatgtt
    15841 gatggcatga agacaggaac cacggcaggc gcgggatata atctggttgc ttcggctacc
    15901 cagggcgata tgcgtttaat ctccgtagtg ctgggggcga aaaccgaccg tatccgtttt
    15961 aatgagtctg agaaattatt gacctggggt ttccgcttct ttgaaaccgt gacgccaatt
    16021 aaacctgatg ccacctttgt gactcagcgc gtctggtttg gtgataagag cgaagtgaat
    16081 ctcggggcag gcgaagcggg ctcagtgacc ataccgcgtg ggcagctgaa aaacctgaag
    16141 gcgagctaca cgttaacgga accgcagctt accgcaccgc tgaaaaaagg tcaggttgtc
    16201 gggaccattg atttccagct taacggtaaa tccattgagc agcgtccgct gatcgtgatg
    16261 gaaaatgtgg aagagggcgg attctttggt cggatgtggg atttcgtgat gatgaaattc
    16321 catcagtggt tcggcagctg gttctcttaa tcttctgata accggatggc gcgaaacgtc
    16381 atccggttat acgtcattaa tacatcaact taatacgctg cgtctgcgcg tacttcacat
    16441 actcatcttc cgggcaacaa tcgctcacca caatatcaaa gcgtttcagg tcacccatgc
    16501 gcgccggacg caccttgcca aatttactgt ggtcaacaac cagcacatgc ttttgcgcca
    16561 tcgacatggc ccagtgtttt accggcaact cttcaagatt aaaacaggta gcgcctttac
    16621 tgacatgcac gcccgccgca gaataaaaag cgatatccgg gcaaaaatta ttcagcgttt
    16681 gctgaaaatc gatgggtttg aaaatggcgt tgctggcgtg aaattcacca ccgcacagaa
    16741 atgcgcggca atggggtttt tctttcagcg ccagaaaggt atttagcgaa taacaaacgg
    16801 cggtaaaagg gatttcatta tcaatcgctt caataatcca cggcgtggtg gtgccacagt
    16861 caaaaaagag ggtctgatcg ggttctacca gtgtcgcagc cagttttgcc gcccggcgtt
    16921 tttcttccac caggcgggat ttttgatcgc ttaacaggta atggctggcg ctgcgcggtt
    16981 ccagaacaat atagccgccg agcaaaacga cgggcgcact gtggttgttc agatcgcgac
    17041 gaatcgtcat ctccgaaacc ccaagcaggg cggcggcgtc tttaagatgt aacttatcgc
    17101 tgcgttttaa ttcttgcagc agctgcccga tacgctcttc gcgacgtgtt tccataatcc
    17161 ctctgaatag ttattgaagc gagccgctca atactacact ttttagcaga gatcagtcac
    17221 gcacccagcc tttgcggatc ggtaatgcaa aacagaagcg ataccacgat tgcagacgtt
    17281 gatagagttt atgccccatc gcttgccaga taatctgggc actaaggcag ccaatcatac
    17341 ctgccagcaa tccaccgagc atatccagcg gccagtggac gccaagataa acgcgcgacc
    17401 aggcaatgac gacggccagc accattaaaa gtgagccgga ccacaggcga tgccagcata
    17461 aaaatgccag tgcaaaggtg aaaatcaccg taccgtgatc gctcgggaat gagtcatccg
    17521 ccgcatgatg caggaagtta tagccgatat tttcgacaaa cggtcggtcg tgcggaaaaa
    17581 gatgtcccat cgtccaggac accaacaggc tgaccgccag cgcgatagcg atttttatca
    17641 ccagttgccg ttgtgctgta agcccccaca accaaagtac cgcggccagc agcggcacca
    17701 cggtaatcaa atctttagca ataaaaatcg ccaacgagat catccacgga gccgagtctg
    17761 gcgtcgcgtt aataagagag aatagagaga gattcaaatt ttccagcata acttcccgac
    17821 gcaaagtgat taaaagggag ccaatacagg caagtcgttg agaataaagt gcaggttaaa
    17881 ctgggtaaag cggcatcgtc ttatttccct caagcggcct gtttacggtg ggtgattgta
    17941 acgggcatag gttaaataaa acttaaagaa agcgtagcta tactcgcaat aatgtaagaa
    18001 tgtgcttaac cgtggtttca gctacaaaat tcgctttctc gttagctgcg cttttattaa
    18061 actctgcgcg attattattg gcgaagaaat tgcatgcaaa ataaattagc ttccggtgcc
    18121 aggcttggac gtcaggcgtt acttttccct ctctgtctgg tgctttacga attttcaacc
    18181 tatatcggca acgatatgat tcaacccggt atgttggccg tggtggaaca atatcaggcg
    18241 ggcattgatt gggttcctac ttcgatgacc gcgtatctgg cgggcgggat gtttttacaa
    18301 tggctcctgg ggccgttgtc ggatcgtatt ggtcgccgtc cggtgatgct ggcgggtgtg
    18361 gtgtggttta ttgtcacctg tctggcaata ttactggcgc aaaacattga acaattcacc
    18421 ctgttgcgct tcttgcaggg cataagcctc tgtttcattg gcgctgtggg atacgccgca
    18481 attcaggaat ccttcgaaga ggcggtttgt atcaagatca ccgcgctgat ggcgaacgtg
    18541 gcgctgattg ctccgctact tggtccactg gtgggcgcgg cgtggatcca tgtgctgccc
    18601 tgggaaggga tgtttgtctt gtttgccgca ttggcagcga tctccttttt cggtctgcaa
    18661 cgagccatgc ctgaaaccgc cacgcgtata ggcgagaaac tgtcgctgaa agaactcggt
    18721 cgtgactata agctggtgct gaagaacggt cgctttgtgg cgggggcgct ggcgctggga
    18781 ttcgttagcc tgccgttgct ggcgtggatc gctcagtcgc cgattatcat cattaccggc
    18841 gagcagttga gcagctatga atatggtttg ctgcaagtgc ctattttcgg ggcgttaatt
    18901 gcgggtaact tgctgttagc gcgtctgacc tcgcgccgca ccgtacgttc gctgattatt
    18961 atgggcggct ggccgattat gattggtctg ttggtcgctg ctgcggcaac ggttatctca
    19021 tcgcacgcgt atttatggat gaccgccggg ttaagtattt atgctttcgg tattggtctg
    19081 gcgaatgcgg gactggtgcg attaaccctg tttgccagcg atatgagtaa aggtacggtt
    19141 tctgcggcga tgggaatgct gcaaatgctg atctttaccg tcggtattga aatcagcaaa
    19201 catgcctggc tgaacggggg caacggactg tttaatctct tcaaccttgt caacggaatt
    19261 ttgtggctgt cgctgatggt tatcttttta aaagataaac agatgggaaa ttctcacgaa
    19321 gggtaaaaaa atgcctgatt gctttgtgcg atcaggcatt ctcgaattaa tggtgatggt
    19381 cgtcaatctg gtgttcgata accatccctt cacctacgct ggcaagatgg cgaacataag
    19441 gatgcgggtg gtaagccgga gctggcgcag gagccacata gacggtttgc ggcacagtcg
    19501 cgacgctgac cgcttgtgga acgctgactg aatcaggaac cacaaccact ttgtatccac
    19561 tcggcacatc gacggtgaca ctttgcgcca tcgccacacc agtaaagccc attaaaagcg
    19621 cgcccaacag tagacaattt ttcataatca tttcgcctga aataatgcag cgggttaatt
    19681 tccgcgaatt atgcagcctt cattcaggta ttgtgttgcc gggcgtgctg ccgtttttat
    19741 tggtcaaatg gcgcttcgtg tttaagaact ttatcgatca catccagtac gccttcacgg
    19801 ttattggagc ctgcccggta ttttgccgct gcgacgaccg cgttgccggc attttccatt
    19861 gcaaaactaa agcctgcctg acgcagcatc tcaatatcgt taccgccatc gccaaagacc
    19921 accacttcgc tgtcgtctat tccccataat ttctgcagtt ggcgaaggcc attggcttta
    19981 tgtacgccgg ggataatcag atcgatgctg ccgttgccgg tgtggaccgg caccataata
    20041 tcgccgatgg cctcatgtaa tgttttttgt acttgtggaa tcagttcatc ggaaagattc
    20101 aggccaaact taaagaagat atcttctaag ttgtcaaagt tatcgacgta ttccagacgg
    20161 tgataataca tttccgccac cattttcatg gcatcgtcat attttttgag cgtataggcg
    20221 ctattttttc cgcaggcaat aatttccact tccgggcgcg tcagcaaatg ttccacgaca
    20281 gtagtaaacg catccttcga cagctcgcca ttaaaaacat ctttgccttc actcactacc
    20341 cagccgccgt tttcagccac aaaggcaatt tcattagcaa tttcagggaa gaaagagatc
    20401 aactgataat attgattccc gctggcgacc acaaagcgaa tgccttgtgc tttcatttgc
    20461 tgatactgag ccataaaccg ctcacggtta taggtttttt gatcgcttaa gaaagtacca
    20521 tccatgtcta ccgcaattaa tttaatgctc atcaactatt ctccatcgcc gtctgcgttt
    20581 tggtatcggg tttggcgacg gctttcgcga caatagccgc gagaataacc agcgccagta
    20641 caaccagcat tgcactacgt aatccataat gttcgccgag atagcccagc agcggcggcc
    20701 cgacgaggaa agccagataa ccggtcgttg ctaccacact gacgcgggtt ggtgcatcgg
    20761 ggccggtatc gctggcggca gaaatggtca gcgggaagcc cagcgaggca cccagtcccc
    20821 agagtacaac agacacccca gcgacccagg cgctatcgac aaaaataatc agcccaatac
    20881 ccaacgcccc cattagcgca ctggcccgaa ccacggcaac gcgactgtaa cggtcgatga
    20941 accaaccgcc agtgaagcgt ccaacggtca tccccagggt aaaaccggca taaatcagcg
    21001 agccggaagt ggggctaaaa ccgtgaccgt caaccattaa taagggtaac cagtcgttgg
    21061 cagaaccttc ggcaaaggcc atcgccagca ccacaacacc aatcagcagc aactggatat
    21121 cgcgataaaa aggtacgcct ttttcgccat gctgggtgcc atcggcagca tttttgcccg
    21181 taccgtcagg gattgcctga atggcgatat atataggcgc gatgcctacc agcgccgcca
    21241 ataaaatgtg caccgttgcc ggaacgccaa aggccgtcag tgccatcccg acaccagcgc
    21301 ctgccagcgt gcccaggcta taaaaaccgt gcatcatcgg caaaaccgtt ttattcattt
    21361 ctcgctcaac ggcggcacct tcaacgttta tcgccacttc cgcagaacca aaacttgccc
    21421 caaagacgcc gagaccaacg gcaaagagca ggggcgatgt cagccagagt gccagactta
    21481 atatcatcat cccgaccaat gcgcaggaca tggtgaccag gatgacatta cgtgtcccaa
    21541 agcgtttcac taaccacgcc gagcagagaa taccgctcat cgaaccgatc gacagaccaa
    21601 agagaacacc gcccatttca gcgatcgaga cagagagaat atcgcggata gcaggcgtac
    21661 gggttgccca ggacgccatt aacaggcccg gcaaaaagaa gaacataaac agcgcccagg
    21721 ttcggcgttt caatgcatta cgtgaagaat ttacggtcat agatcacgtc aaaataagaa
    21781 gagggaagac aacattagca aggttgtgta catttgtaca caattgcaga cagaggaaat
    21841 gacatgcgtc gcgctaacga tccgcaacgg cgagaaaaaa ttatccaggc cacgctggag
    21901 gcggtgaaac tttacggaat acatgctgtt acgcaccgca aaattgctac ccttgccggg
    21961 gtaccgttgg gatcgatgac ctactatttt tcaggaattg atgagttgtt gctggaggcg
    22021 ttcagcagtt ttactgagat catgtcccgg caatatcagg cattttttag cgatgttagt
    22081 gatgctcaag gcgcatgcca ggctatcacc gatatgatct acagctcaca ggttgcaacg
    22141 ccggataaca tggagctgat gtaccagctc tacgcgctgg ctagccgaaa accgctatta
    22201 aaaacggtaa tgcaaaactg gatgcagcgc agtcagcaaa cgctcgaaca atggtttgaa
    22261 cccggaaccg cccgcgcgct tgatgcgttt attgagggga tgacgctgca ttttgtcacc
    22321 gaccgtaagc cgctatcgcg cgaggagatt ttgaggatgg ttgagagggt tgcagggtag
    22381 tagataaatt tcaggcaaca aaaaacccat caaccttgaa ccgaaatggc ggggttgatg
    22441 ggctccacaa aatggggaca tcaaagaaaa gcagtggcac taattaagac tgatgccctg
    22501 cggaaaagtt ctgcggttgt gcaaaaaaat ttcattttca gggcaacttc agttttatcc
    22561 taatcctggc cataccatga cgatgattgt ccctgccagc gtcagcagga cgttggcgat
    22621 tgcgtaggtg cccgcatagc ccagcgccgg gatgttactg cgagctgtat cactgatgat
    22681 ctccattgcc ggcgcgcagg tgcgtgcgcc catcattgcg ccgaacaaca gtgcgcggtt
    22741 cattcgcaat acataagcac cgaacaagaa acagataacc acgggcacaa gactgacaat
    22801 caatccggca atcaacatct gaccgccaat cgcgcccaga ccgttattaa taccgctacc
    22861 ggcgctcaga ccaacgcctg ccataaacac catcaagccg aactctttca ccatgcttaa
    22921 tgccccttgc ggaatgtaac cgaaggtcgg gtggttagca cgcataaagc ccagcataat
    22981 tccggcgaat aacaacccgg cagcgttccc catgccgaaa ctgaatgtgc tgaactggaa
    23041 ggtgatcatc ccgatcatca gcccaataac aaagaaggcg cagaatgcca gcaggtcagt
    23101 gacctggctg tgaatcgaga taaagccgat gcgatcggcg atggttttta cgcggcgggc
    23161 atcgccgctg acttgtaaaa cgtcaccttt gttaagcacg acgttgtcat caatcggcat
    23221 ctcaatctgg ctacgaatga cgcggttaag gaagcaaccg tgatcggtca acttcagttg
    23281 tgcgagacgt ttacctacag cgttatggtt tttaacgacc acttcttcag tgacgatacg
    23341 catgtcgaga aggtcacgat cgaaaacttc tttaccgtta cggaagctgg gatcgagtcg
    23401 ggcatgggcg tcgggatagc ctaccaacgc tatttcatcg cccatttgta gcacggcatc
    23461 accgtctgga tttgccagaa tcccgttacg tcgaatacgt tcaatgtagc agccggtttg
    23521 tcgataaata cccagttcac gcagattttt gccgtcggtc caggccacca gctccgggcc
    23581 gacgcgatag gcgcggatca ccggtaaata aaccttacgg ttggcatcag tgtccaggcc
    23641 acgttcgcgg gcgatttgct gggcgctggt ctgtaagtcc tgatgctgca atttcggcaa
    23701 gtaacgcgca ccaacaatca aactcaccag accgattaaa taggttaagg catacccgag
    23761 gctcagatta tccagtgcca gtgagagctg cctgctttcc ataccggaat gacgcagtgt
    23821 atcgccagca ccgaccagaa ccggtgtcga cgtcatagag cctgctaaca taccggccgt
    23881 caggccaata tcccagccaa acagcttacc taaccctaag gcgatcacca gcgcactgcc
    23941 aaccatcacc agtgctaaca ttaggtaatt tttcccatcg cgaaaaaaaa tggaaaaaaa
    24001 gttcggtccg gcttcgaccc cgacgcagaa aataaacagc ataaagccaa gattaagcgc
    24061 atcggtgtta atgctgaaat gttgttggcc taataacagc gatacgacta aaacgccaat
    24121 ggaattaccc agttggatcg aaccaagtcg taactttccg agacatagcc caagcgcgag
    24181 gaccacaaat aataacagaa tgtaattccc atttaacaat tcggcgacgt ttatattcac
    24241 ggaggctaac ttcttgttta ctagtaagct gttgaaagaa atggtaattt atgataatgt
    24301 tttttaccag aattcagggc gcagattcat tcagcgcacc taaacgatag taaagtaaca
    24361 atatatttta ctagtgtaat cacattaggt atcaacggct atatgaattg cgttggccta
    24421 tattagcatg gaatgcgaag cggctttatc ttactgaacg ccacactggc gaaaaatgtg
    24481 ttcgatagac gcagtgtcag gaggaacgag tgaaacataa acaacgttgg gcgggggcaa
    24541 tctgctgttt tgtcctcttc attgtggtgt gcctttttct ggcgacgcac atgaaaggcg
    24601 cttttcgggc tgccgggcat cctgaaatcg gcttgctgtt tttcattctt cctggagcag
    24661 ttgccagttt cttttcacag cgtagagaag tcctgaaacc tctatttggc gcaatgctgg
    24721 cggcaccctg ttcaatgctt attatgcggc tgtttttttc accgacccgc tcattctggc
    24781 aagagctggc atggttacta agcgcggtgt tctggtgtgc gctgggggca ctgtgtttct
    24841 tatttatcag tagcttgttt aaaccacagc acagaaaaaa tcagtaaagc cctcaacgcg
    24901 agggcttgtc agacgatcag gcgtccagat tctctttcac ccatgcagca aaatcggtat
    24961 agccgccgat atgttgctga tcgacaaaaa tctgcggcac ggtttctacg ggtttacctg
    25021 ccttttgttg tagatcttct ttagtgatcc cttccgcacg aatatctaca tactgatact
    25081 gaaaatcatc gcgttcattg ctcaatttct cagccagatc ttttgcacgc acacagtaag
    25141 ggcaacccgg acgaccaaaa ataacggttt gcattatttc tctcctcata gatttatgcc
    25201 tgtaatgatc acgctaaaat gtattcgctg aaagtaggtt taacctgttg cattaattgc
    25261 taaaagctat aactgttaaa cacaatacag tgaaaagttt tagactgaag gttcactttg
    25321 cagagggaag cgtatgcgcg cgatcggtaa attgcctaaa agcgtgttga tactggaatt
    25381 tatcggaatg atgttgctgg cggtggcgct gctgtcggta agcgactccc tgtcgctgcc
    25441 tgagccattt tctcggccag aagtgcagat tctgatgatt tttctcggtg ttttgctcat
    25501 gcttcccgct gcggtggtgg ttattcttca ggtggcaaaa cgtcttgccc cacagctgat
    25561 gaaccgtcca ccgcagtatt cacgttcaga aagagaaaaa gataatgacg ccaaccattg
    25621 aacttacttg tggccatcgc tccattcgcc atttcactga tgaacccatt tccgaggcgc
    25681 agcgtgaggc gattattaac agcgcccgtg cgacgtccag ttccagtttt ttgcagtgca
    25741 gtagcattat tcgcattacc gacaaagcgt tacgtgaaga actggtgacg ctgaccggcg
    25801 ggcaaaaaca cgtagcgcaa gcggcggagt tctgggtgtt ctgtgccgac tttaaccgcc
    25861 atttacagat ctgtccggat gctcagcttg gcctggcgga acaattgctg ctcggtgtcg
    25921 ttgatacggc aatgatggcg cagaatgcat taaccgcagc ggaatcgctg ggattgggcg
    25981 gggtatatat cggcggcctg cgcaataata ttgaagcggt gacgaaactg ctgaaattac
    26041 cgcagcatgt tctgccgctg tttgggctgt gccttggctg gcctgcggat aatccggatc
    26101 ttaagccgcg tttaccgtcc tccattttgg tgcatgaaaa cagctatcaa ccgctggata
    26161 aagacgcact ggcgcagtat gacgaacaac tggctgaata ttacctcacc cgtggcagca
    26221 ataatcgccg ggatacctgg agcgatcata tccgccgaac aatcattaaa gaaagccgcc
    26281 catttattct ggattatttg cacaaacagg gttgggcgac gcgctaaaac cgccacgccg
    26341 atgtatgata cgcgggcttt tgaccaggtc tgacagagag gtgcagggtg aaaattgcca
    26401 tattgtcccg ggatggaacg ctctattcgt gtaagcggct gcgtgaagcc gctatacagc
    26461 gcggtcacct ggttgaaatt cttgatccgc tttcttgcta catgaacata aatcctgcgg
    26521 cgtcttctat tcactacaaa ggccgcaagt taccccattt tgacgcagtg atcccgcgta
    26581 ttggcaccgc cattaccttt tatgggacgg cggcactgcg ccagttcgag atgctgggga
    26641 gctatccgct caatgagtcg gtcgccattg cccgggcgcg tgacaaattg cgttccatgc
    26701 aactgctggc gcgtcagggc atcgacctgc ctgtcacggg cattgcgcat tcgccggatg
    26761 ataccagcga tttaatcgac atggtcggtg gtgcgccgct ggtggtcaag ttggttgaag
    26821 gcacgcaggg aattggcgtc gtgctggcgg agacgcgtca ggcggcggaa agcgtgattg
    26881 acgctttccg cggtctgaac gcgcatattc tggtgcagga atatatcaaa gaggcgcaag
    26941 ggtgcgatat ccgctgtctg gttgttggcg atgaagtggt cgctgcgatt gaacggcggg
    27001 cgaaagaggg cgattttcgt tccaatttgc atcgtggcgg cgcggcaagc gtcgccagta
    27061 tcacaccaca ggagcgtgaa atcgcgataa aagccgcgcg aacgatggcg ctggacgttg
    27121 ctggtgtgga tattctgcgt gctaatcgcg ggccgttggt gatggaagtg aatgcgtcgc
    27181 cggggctgga aggaatagaa aaaaccaccg gtatcgacat cgcgggtaaa atgatccgct
    27241 ggatcgaacg ccacgctacg acagaatatt gcctgaaaac gggtggttag tcgcaatcac
    27301 attactgatc atggttttgc ctgctctttt tgcgtaagct gtgccggtct ttttatcgaa
    27361 agaggttgta caaaattatg acatcgctgg tcgttcctgg tctggatacg ctgcgtcaat
    27421 ggctcgatga cctggggatg agtttttttg aatgtgataa ctgtcaggct ctgcatctgc
    27481 cccatatgca gaatttcgac ggtgtctttg atgccaaaat cgatctgatc gataacacga
    27541 tcctgttttc tgccatggcg gaagtccgac cttcagccgt attgccgctg gcggcggatt
    27601 tatctgccat caatgccagt tcgctgaccg tgaaagcatt tcttgatatg caggatgata
    27661 atctgccaaa gctggtggtt tgccagtctt tatccgttat gcagggcgta acctatgagc
    27721 agtttgcatg gttcgtgcgt cagagcgaag agcagatttc gatggtcatt cttgaagcta
    27781 atgcccatca actgctgtta ccgactgacg atgaagggca aaacaacgtt accgaaaact
    27841 atttcctcca ctgataactc ctttcgagca cgcagtcgct ggtgcagtgg ctgcgcgctg
    27901 caaaattatc tgctgttttt aaccttttct taaagattat ttcacttctc ttgtgtcgat
    27961 ttggctttat cacatagagc aaatacgcat aaaaatttgt taaataccgt tttttaatcc
    28021 gagctatagt ctcaaaccct ggctaaagtt attcttgcga tgcttttata tagcgagcag
    28081 tgctggccgg gagagagttc tcttttctta caccgcgccg ataaaaaata tgcacgttta
    28141 ttgcatatct ttcagtgtga caacttttgt tcgtttgtta acgaactttc agaaggaaag
    28201 agatatgacc gccttaaata aaaaatggct ttcgggtctg gttgcgggtg ctctgatggc
    28261 cgtctctgtc ggcacgctcg cggctgaaca aaaaacactc cacatttata actggtctga
    28321 ttatatcgcc cccgacacgg tggctaactt tgaaaaagaa accggtatta aagtcgtcta
    28381 cgatgttttc gactctaacg aagtactgga aggcaaatta atggccggga gcaccggctt
    28441 tgacctggtg gtgccgtcag caagctttct tgaacgccag ctgacagcgg gagttttcca
    28501 gccgctggat aaaagcaaat tgccggagtg gaagaatctc gacccggaac tgctgaagtt
    28561 ggtcgccaaa cacgatcccg acaataaatt tgctatgccc tatatgtggg cgacgaccgg
    28621 gattggctat aacgtcgata aagtcaaagc ggtgctgggc gaaaacgcgc ccgtcgatag
    28681 ctgggacttg atcctcaagc ctgaaaatct ggaaaaactg aaaagctgcg gtgtatcttt
    28741 cctcgatgcg ccagaagaag tttttgctac cgtgctgaat tatctcggca aagatcccaa
    28801 cagcactaaa gcggatgatt acaccggacc ggctaccgat ctgctgttaa agctgcgccc
    28861 gaacattcgt tatttccatt catctcaata tattaacgac ctggcaaacg gcgatatttg
    28921 cgtcgctatc ggctgggcag gtgatgtctg gcaggcgtca aaccgcgcga aggaagcgaa
    28981 gaatggcgtg aatgtctcgt tctcgattcc aaaagaaggg gcgatggcgt tctttgatgt
    29041 attcgccatg cctgcggatg ccaaaaataa agacgaagcc tatcagttcc tgaattacct
    29101 gctgcgtcct gatgtggtgg cacatatctc tgaccatgtg ttctatgcta acgccaataa
    29161 agcagccacg ccgctggtga gtgcggaagt ccgtgataac ccgggtattt atccgcctgc
    29221 ggatgtccgc gcgaagttgt tcactctgaa agtacaggct ccgaaaatcg accgtgtgcg
    29281 cacccgcgcg tggaccaaag tgaagagcgg aaaataatcc gcagccttag atgccgggcg
    29341 ggagcgccac tccctccggc aattcgcacc aacatggtgc gcttgcacac atttaatgcc
    29401 ggagagcagc cgtgaatgac gctatccctc gcccccaggc gaaaacccgt aaggcgctga
    29461 ccccgctatt agaaatccgc aatctgacca aatcctacga tggtcagcat gcggttgatg
    29521 atgtcagcct gaccatctac aaaggtgaaa tctttgcgct gctgggcgca tccggctgtg
    29581 gcaagtccac gctgctgcgt atgctggcag gtttcgaaca accttcagcc gggcagatta
    29641 tgcttgatgg cgtcgatttg tcacaggtgc cgccttacct gcgccccatc aatatgatgt
    29701 ttcagtctta cgcgctgttt ccgcatatga ccgtggaaca gaacatcgct tttggcctga
    29761 aacaggacaa actaccgaaa gcggaaattg ccagccgggt caatgagatg ctcggccttg
    29821 tgcatatgca ggagttcgcc aaacgcaaac cgcatcagct ttccggtggt cagcgacagc
    29881 gtgtggcact ggcccgaagc cttgccaagc gcccgaaact attactgctc gacgagccga
    29941 tgggcgcgct ggataaaaag ctgcgtgacc ggatgcagct tgaagtggtg gatattctgg
    30001 aacgcgtcgg tgtgacttgt gtgatggtca cccacgatca ggaagaggcg atgaccatgg
    30061 cggggcgtat cgccattatg aatcgtggga aatttgtcca gattggcgaa ccggaagaga
    30121 tctacgagca tccgactacc cgctatagcg ccgaatttat tggctcggtt aatgtctttg
    30181 aaggtgtact caaagagcgt caggaagatg gtctggtgct ttattcgccg gggctggtgc
    30241 atccactgaa agtcgatgcg gatgcttcag tggtcgataa cgtgccggta cacgtagcgc
    30301 tgcgtccgga aaaaatcatg ctttgcgaag agccgcccgc caatggttgt aacttcgcgg
    30361 tgggggaggt gatacacatt gcctatctcg gcgatctttc ggtgtatcac gttcgtctga
    30421 aaagtgggca gatgattagc gcccagctac aaaacgccca tcgtcatcgt aaagggttac
    30481 cgacctgggg cgacgaagtg cgtttgtgct gggaagtgga cagctgtgtg gtgctgacgg
    30541 tttaaggagc aaagatgagt acacttgaac ctgctgccca gtcgaaaccg ccgggcggat
    30601 ttaagctgtg gttgtcgcag ttgcaaatga agcatgggcg caaactggtc attgcgttgc
    30661 cgtatatctg gttgatcttg ctgtttctgc tgccatttct gattgtcttt aaaataagcc
    30721 tggcggagat ggcgcgcgct attccacctt ataccgaact gatggagtgg gctgacgggc
    30781 aactgtccat cacccttaat ttcggtaatt tcctgcaact gaccggcgat ccgctctatt
    30841 tcgatgctta tctccagtcg ttacaggtgg cggcgatttc gacattttgc tgtttactga
    30901 tcggctatcc gctggcgtgg gcggtggcgc acagtaagcc ttcgacccgt aatatcttat
    30961 tactactggt gatcctgccg tcgtggacct cgtttctgat tcgtgtctac gcctggatgg
    31021 gaatattaaa aaacaacggt gtgctgaata attttctgct gtggctgggg gttatcgatc
    31081 aaccgctgac cattcttcat accaatctgg cagtttatat tggcattgtt tacgcctacg
    31141 tgccgtttat ggtgctgccg atttataccg cgttgattcg tattgattat tcgctggtgg
    31201 aagcagcgct ggatctcggt gcacgaccgc tgaaaacgtt ctttactgtg atcgtgccgc
    31261 tgactaaagg tgggattatt gccggatcga tgctggtgtt tatcccggct gtgggcgagt
    31321 ttgtgatccc ggaactgctc ggtggcccgg acagcatcat gatcgggcgc gtgctatggc
    31381 aagagttttt caataaccgc gactggccgg tggcctcggc ggtagcgatc atcatgttgc
    31441 tgctgctaat tgtgccgata atgtggtttc acaaacacca gcaaaaaagc gtgggagaac
    31501 acggatgaat aatttaccgg tagttcgttc gccctggcgg attgtgattt tgctgttggg
    31561 cttcaccttt ctctacgcgc caatgctgat gctggttatc tattcgttta acagctcgaa
    31621 gctggtgacg gtgtgggccg gctggtcaac gcgctggtat ggcgaattat tgcgcgatga
    31681 cgcgatgatg agtgcggttg gtttaagtct gacaattgcg gcctgtgcgg cgacggcggc
    31741 ggcgatcctc ggaactattg cggcggtggt gctggtgcgc tttggcaggt ttcgcggatc
    31801 aaatggcttt gcctttatga tcaccgcgcc gctggtaatg ccagatgtca tcacgggctt
    31861 gtcgctgttg ttattattcg tcgcgcttgc tcatgccatt ggctggcctg cggaccgcgg
    31921 tatgcttacc atctggctgg cgcatgtcac tttctgtacg gcttatgtgg cagtcgttat
    31981 ttcgtcgcgt cttcgggaac tggatcgctc gatagaagaa gcagcgatgg atctcggtgc
    32041 gacgccgctg aaagtgtttt tcgtcattac gctaccgatg atcatgcccg cggtcatttc
    32101 tggctggtta ctggctttta ctttgtcgct tgatgatctg gtgatcgcca gctttgtatc
    32161 cgggccggga gccaccacgt taccgatgct ggtcttttcc agcgtgcgga tgggggtgaa
    32221 tccggaaatc aacgccctag caacattgat cctcggcgcg gtcggaattg tcggatttat
    32281 cgcctggtat ctgatggctc gcgcagaaaa acagcggata cgcgacatcc agtgtgcaag
    32341 acgtggctga agacactaaa atttgccaac ctggctacat aatgccgcgt atgtcgcggc
    32401 attgttttca tggaagacga aacgttggga ttttttaaga aaacatcttc atctcatgct
    32461 cgcctgaatg tgcctgcgct tgtgcaggtg gcggcgctcg ccattattat gatccgtggc
    32521 ctcgacgtgc tgatgatttt caatacgctg ggcgtgcgcg gtattggcga gttcattcat
    32581 cgcagcgtac aaacctggag tttaacgctg gtctttttaa gcagtctggt gctggttttc
    32641 attgagatct ggtgtgcgtt ttcactggtg aaagggcgtc gctgggcgcg ctggctgtat
    32701 ctgctgacac aaatcactgc cgcaagttac ttgtgggcgg cttcgctggg gtacggttat
    32761 ccggagctgt tcagcattcc cggtgaatca aaacgtgaaa tcttccatag cctgatgctg
    32821 cagaagctgc cggatatgct catcctgatg ctgctgttcg ttccctcgac cagtcggcgg
    32881 ttcttccagt tgcaataatg tgtataatcg tcgcccctga tgatgtgaag gtcaatgtat
    32941 gcagtgcgca ctttacgacg cgggtcgctg tcgttcctgt cagtggataa cgcagccgat
    33001 tccagagcaa ctctccgcta aaaccgccga tcttaaaaat ctgctcgccg actttccggt
    33061 tgaggaatgg tgcgcgccgg tgtcaggccc ggaacaaggg tttcgtaata aagccaaaat
    33121 ggtggtgagt ggtagcgttg aaaaaccact gctcggtatg ctgcatcgcg acggcacacc
    33181 ggaagacctt tgtgactgcc cgctttatcc tgcctcattt gcgcccgttt ttgcggcgct
    33241 aaaaccgttt atcgcccgag cggggttaac gccctacaac gtggcgcgta agcgtggcga
    33301 actgaaatac attctgctga ctgaaagcca gagcgatggc ggcatgatgc tgcgctttgt
    33361 actgcgttct gaaaccaaac tagcacaact gcgtaaggca ttgccgtggt tacaggcaca
    33421 attgccgcag ctaaaagtta tcaccgtcaa tattcagccg gtacatatgg cgattatgga
    33481 aggggagacg gagatctacc tgaccgaaca acaggcgctg gtagagcgtt ttaacgatgt
    33541 gccgctgtgg atccgtccgc aaagtttctt ccagactaat ccggcggtcg ccagccagct
    33601 gtacgctacc gcgcgcgact gggtacgaca gctgccggtt aaacatatgt gggatctgtt
    33661 ctgcggtgtg gggggctttg gtttacactg cgcgacgcct gacatgcagt taaccgggat
    33721 cgaaattgcg ctagaggcca ttgcctgtgc aaagcagtca gccgctgaac tgggcttaac
    33781 gcgtctgcaa tttcaggcgc tggactccac tcagtttgcc accgctcagg gggaggtgcc
    33841 ggagctggtg ctggttaacc cgccgcgccg cggcattggt aaaccgctgt gtgattatct
    33901 ctcaacgatg gcaccgcgtt ttattatcta ctccagctgt aacgcccaaa ccatggcgaa
    33961 agatatccgc gaactgccag gttaccgcat cgaacgggta cagctttttg atatgttccc
    34021 gcacaccgcg cactatgaag tgctgacgct gctggtaaaa atgtaaatac atcattcaaa
    34081 tcaatatcac ctcggcatac atccatccaa aataataatc gtgatcgatc gattaataat
    34141 aac
//
LOCUS       DABSYT020000043        33086 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.043, whole genome shotgun sequence.
ACCESSION   DABSYT020000043 DABSYT020000000
VERSION     DABSYT020000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.043"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            13..1344
                     /gene="argA"
                     /locus_tag="ID725_RS12400"
                     /old_locus_tag="pgaptmp_002480"
     CDS             13..1344
                     /gene="argA"
                     /locus_tag="ID725_RS12400"
                     /old_locus_tag="pgaptmp_002480"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGV7851061.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKSCRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(1406..3232)
                     /gene="recD"
                     /locus_tag="ID725_RS12405"
                     /old_locus_tag="pgaptmp_002481"
     CDS             complement(1406..3232)
                     /gene="recD"
                     /locus_tag="ID725_RS12405"
                     /old_locus_tag="pgaptmp_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGV7851062.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(3232..6774)
                     /gene="recB"
                     /locus_tag="ID725_RS12410"
                     /old_locus_tag="pgaptmp_002482"
     CDS             complement(3232..6774)
                     /gene="recB"
                     /locus_tag="ID725_RS12410"
                     /old_locus_tag="pgaptmp_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGV7851063.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(6767..9655)
                     /gene="ptrA"
                     /locus_tag="ID725_RS12415"
                     /old_locus_tag="pgaptmp_002483"
     CDS             complement(6767..9655)
                     /gene="ptrA"
                     /locus_tag="ID725_RS12415"
                     /old_locus_tag="pgaptmp_002483"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGV7851064.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(9831..13199)
                     /gene="recC"
                     /locus_tag="ID725_RS12420"
                     /old_locus_tag="pgaptmp_002484"
     CDS             complement(9831..13199)
                     /gene="recC"
                     /locus_tag="ID725_RS12420"
                     /old_locus_tag="pgaptmp_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGV7851065.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(13212..13535)
                     /gene="ppdC"
                     /locus_tag="ID725_RS12425"
                     /old_locus_tag="pgaptmp_002485"
     CDS             complement(13212..13535)
                     /gene="ppdC"
                     /locus_tag="ID725_RS12425"
                     /old_locus_tag="pgaptmp_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGV7851066.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(13520..13927)
                     /gene="ygdB"
                     /locus_tag="ID725_RS12430"
                     /old_locus_tag="pgaptmp_002486"
     CDS             complement(13520..13927)
                     /gene="ygdB"
                     /locus_tag="ID725_RS12430"
                     /old_locus_tag="pgaptmp_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGV7851067.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(13924..14487)
                     /gene="ppdB"
                     /locus_tag="ID725_RS12435"
                     /old_locus_tag="pgaptmp_002487"
     CDS             complement(13924..14487)
                     /gene="ppdB"
                     /locus_tag="ID725_RS12435"
                     /old_locus_tag="pgaptmp_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGV7851068.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSLVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(14478..14948)
                     /gene="ppdA"
                     /locus_tag="ID725_RS12440"
                     /old_locus_tag="pgaptmp_002488"
     CDS             complement(14478..14948)
                     /gene="ppdA"
                     /locus_tag="ID725_RS12440"
                     /old_locus_tag="pgaptmp_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGV7851069.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(15132..15926)
                     /gene="thyA"
                     /locus_tag="ID725_RS12445"
                     /old_locus_tag="pgaptmp_002489"
     CDS             complement(15132..15926)
                     /gene="thyA"
                     /locus_tag="ID725_RS12445"
                     /old_locus_tag="pgaptmp_002489"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGV7851070.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(15933..16808)
                     /gene="lgt"
                     /locus_tag="ID725_RS12450"
                     /old_locus_tag="pgaptmp_002490"
     CDS             complement(15933..16808)
                     /gene="lgt"
                     /locus_tag="ID725_RS12450"
                     /old_locus_tag="pgaptmp_002490"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGV7851071.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(16959..19205)
                     /gene="ptsP"
                     /locus_tag="ID725_RS12455"
                     /old_locus_tag="pgaptmp_002491"
     CDS             complement(16959..19205)
                     /gene="ptsP"
                     /locus_tag="ID725_RS12455"
                     /old_locus_tag="pgaptmp_002491"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGV7851072.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(19218..19748)
                     /gene="rppH"
                     /locus_tag="ID725_RS12460"
                     /old_locus_tag="pgaptmp_002492"
     CDS             complement(19218..19748)
                     /gene="rppH"
                     /locus_tag="ID725_RS12460"
                     /old_locus_tag="pgaptmp_002492"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGV7851073.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(20101..20247)
                     /gene="ygdT"
                     /locus_tag="ID725_RS12465"
                     /old_locus_tag="pgaptmp_002493"
     CDS             complement(20101..20247)
                     /gene="ygdT"
                     /locus_tag="ID725_RS12465"
                     /old_locus_tag="pgaptmp_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGV7851074.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            20433..21122
                     /gene="mutH"
                     /locus_tag="ID725_RS12470"
                     /old_locus_tag="pgaptmp_002494"
     CDS             20433..21122
                     /gene="mutH"
                     /locus_tag="ID725_RS12470"
                     /old_locus_tag="pgaptmp_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGV7851075.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            21191..21904
                     /gene="ygdQ"
                     /locus_tag="ID725_RS12475"
                     /old_locus_tag="pgaptmp_002495"
     CDS             21191..21904
                     /gene="ygdQ"
                     /locus_tag="ID725_RS12475"
                     /old_locus_tag="pgaptmp_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGV7851076.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            22042..22260
                     /gene="ygdR"
                     /locus_tag="ID725_RS12480"
                     /old_locus_tag="pgaptmp_002496"
     CDS             22042..22260
                     /gene="ygdR"
                     /locus_tag="ID725_RS12480"
                     /old_locus_tag="pgaptmp_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGV7851077.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            22368..23408
                     /gene="tas"
                     /locus_tag="ID725_RS12485"
                     /old_locus_tag="pgaptmp_002497"
     CDS             22368..23408
                     /gene="tas"
                     /locus_tag="ID725_RS12485"
                     /old_locus_tag="pgaptmp_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGV7851078.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(23440..24633)
                     /gene="lplT"
                     /locus_tag="ID725_RS12490"
                     /old_locus_tag="pgaptmp_002498"
     CDS             complement(23440..24633)
                     /gene="lplT"
                     /locus_tag="ID725_RS12490"
                     /old_locus_tag="pgaptmp_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGV7851079.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(24626..26785)
                     /gene="aas"
                     /locus_tag="ID725_RS12495"
                     /old_locus_tag="pgaptmp_002499"
     CDS             complement(24626..26785)
                     /gene="aas"
                     /locus_tag="ID725_RS12495"
                     /old_locus_tag="pgaptmp_002499"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGV7851080.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLRAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            27371..28402
                     /gene="galR"
                     /locus_tag="ID725_RS12500"
                     /old_locus_tag="pgaptmp_002500"
     CDS             27371..28402
                     /gene="galR"
                     /locus_tag="ID725_RS12500"
                     /old_locus_tag="pgaptmp_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGV7851081.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(28409..29671)
                     /gene="lysA"
                     /locus_tag="ID725_RS12505"
                     /old_locus_tag="pgaptmp_002501"
     CDS             complement(28409..29671)
                     /gene="lysA"
                     /locus_tag="ID725_RS12505"
                     /old_locus_tag="pgaptmp_002501"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGV7851082.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            29793..30728
                     /gene="lysR"
                     /locus_tag="ID725_RS12510"
                     /old_locus_tag="pgaptmp_002502"
     CDS             29793..30728
                     /gene="lysR"
                     /locus_tag="ID725_RS12510"
                     /old_locus_tag="pgaptmp_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGV7851083.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(30715..31407)
                     /gene="ygeA"
                     /locus_tag="ID725_RS12515"
                     /old_locus_tag="pgaptmp_002503"
     CDS             complement(30715..31407)
                     /gene="ygeA"
                     /locus_tag="ID725_RS12515"
                     /old_locus_tag="pgaptmp_002503"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGV7851084.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(31536..32954)
                     /gene="araE"
                     /locus_tag="ID725_RS12520"
                     /old_locus_tag="pgaptmp_002504"
     CDS             complement(31536..32954)
                     /gene="araE"
                     /locus_tag="ID725_RS12520"
                     /old_locus_tag="pgaptmp_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGV7851085.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLS
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8108 a         9095 c         8203 g         7680 t
ORIGIN      
        1 aagaggtgtg ccgtggtaaa ggaacgtaaa accgagctgg tcgagggatt ccgccattcg
       61 gttccctata tcaataccca ccggggaaaa acgtttgtca tcatgctcgg cggtgaagcc
      121 attgagcatg agaatttctc cagtatcgtt aatgatatcg ggttgttgca cagcctcggc
      181 atccgtctgg tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct ggctgcgcat
      241 caccacgaac cgctgtatca caagaatata cgtgtgaccg acgccaaaac actggaactg
      301 gtgaagcagg ctgcgggaac attgcaactg gatattactg ctcgcctgtc gatgagtctc
      361 aataacacgc cgctacaggg cgcgcatatc aacgtcgtca gtggcaattt tattattgcc
      421 cagccgctgg gcgtcgatga cggcgtggat tactgccata gcgggcgtat ccggcggatt
      481 gatgaagacg cgatccatcg tcaactggac agcggtgcaa tagtgctaat ggggccggtc
      541 gctgtttcag tcactggcga gagctttaat ctgacctcgg aagagattgc cactcaactg
      601 gccatcaaac tgaaagctga gaagatgatt ggtttttgct cttcccaggg cgtcactaat
      661 gacgacggtg atattgtctc cgaacttttc cctaacgaag cgcaagcgcg ggtagaagcc
      721 caggaagaga aaggcgatta caactccggt acggtgcgct ttttgcgtgg cgcagtgaaa
      781 tcctgccgca gcggcgtgcg tcgctgtcat ttaatcagtt atcaggaaga tggcgcgctg
      841 ttgcaagagt tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga aagcgccgag
      901 cagattcgtc gcgcaacaat caacgatatc ggcggtattc tggagttgat tcgcccgctg
      961 gagcagcaag gtattctggt acgccgttct cgcgagcagc tggagatgga aatcgacaaa
     1021 ttcaccatta ttcagcgcga taacacgact attgcctgcg ccgcgctcta tccgttcccg
     1081 gaagagaaga ttggggaaat ggcctgtgtg gcagttcacc cggattaccg cagctcatca
     1141 cggggcgagg ttctgctgga acgcattgcc gctcaggcga agcagagcgg cttaagcaaa
     1201 ttgtttgtgc tgaccacgcg cagtattcac tggttccagg aacgtggatt taccccggtg
     1261 gatattgatt tattgcccga aagcaaaaag cagttgtaca actaccagcg taaatccaaa
     1321 gtgttgatgg cggatttagg gtaagcgatg aaaatcgtcg gatgcgacat gcgtaacact
     1381 cgtacgtcgc atccggcaat tacgtttatt cccgtgaact aaacaacgcc gccagaccac
     1441 tgcgccgctc agtacgagtg gcgattgccg cacttaatat gcgctcatcg gcatacagcg
     1501 acagacggcg acgcgcgcgg gtcaccgcgg tataaaccag ctctcgcgtt actaccggcg
     1561 tgcgttggct cggtaaaatc aacgccgcat ggtcgaactc cgatccctgc gatttatgta
     1621 ccgtcatcgc ccacgtagtt tcgtgctctg gcaagcgact cggttgcaca gacttaatat
     1681 tgccgtccgg catcgcaaac cagacgcgcg tcccctgccc gcgatccagc gcaataccga
     1741 tatcgccatt aaacaaccca agcgcgctgt cattacgggc aatcatcacc ggtcggcctt
     1801 cgtaccaacg agagtgcgga ttacgatgaa ttttgcgctt ctgttgcata aactgctcaa
     1861 ttcgctcatt cagtccagcc acgccaaatg gcccttcccg cagggcgcac aaaagttggt
     1921 actcattgaa cgcctgaatg attaaatccg gctcggcacg cgcttgcagc agatccagat
     1981 aacgtccata acccgcaaga gcttcctcaa gcatcgcaat ataatcttcg ccgctctgta
     2041 aaagccgttt ttcaatatca gtaaaatcct gctgaaaaac ggttttcact gccgttttat
     2101 caccacggtt gatcgccgca gctaactgac caatgccaga atcgctgccg aaacgatagc
     2161 ttttttgcag caggcagaga ctgtcgcgca aagatgccgc ttctgtgcca gttcctgccg
     2221 gaacgtgagt tcccgtcagg cggcttagct gcccggcacg ctcggcggta aagccggcat
     2281 tggcataagc gcagatatcg cccagcacag ccccagcctc aaccgaggcc agttgatcac
     2341 gatcgccgag aaagatcact cgcgcatgat cgggcaaggc gtcgatcagt ctcgacatca
     2401 taggcagatc gatcattgac gcttcatcta ccaccagcac atcaagatgc agcgggttac
     2461 cggcatgatg acgtaaacgc tggctacccg gctgcgcacc cagcaatcgg tgcaaagtac
     2521 tggcatcttc cggaatacgt ttcttttgtt catcggtcag cggtaactgt cgcaaagcct
     2581 tgccgagaga ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc agacggatac
     2641 ggcagcgttc gccgtcggcc atttgaatta acgctgccag caacttcgct acggtggtcg
     2701 ttttaccggt gccagggccg ccggaaatca ccgagatccg ccgcgtcagc gccactgctg
     2761 ccgccacttt ttgccagtta atttcatcac cagtcggaaa gagtttatcc agggtttgcg
     2821 ccaacaaggc ttcatccacc tcgatggcat gattcacctc attgaagaag cgcgctaccg
     2881 tccgctcgtt acaccacatg cgattcaaat aaagacgatc gccacagagg atcatcggcg
     2941 tgggttcatc tccccggctg accgcttgtg aagccagcaa gcattcttcc caattttgta
     3001 gctcaccgat ttcactgaca caggtcgcca acagcggatg cgactcctca ttattttcca
     3061 gtcgtgaaag cggcaaacaa acgtgcccct ctccggcatc atgacttaac agtgccgccg
     3121 cgagggtgac ggcaggatgt tcatctcccg ccacggtcag ggcaaactgc acgtccagcg
     3181 ggcgtagctg tttgtgctcc acagcttcca gtaattgctt ttgcaatttc attacgcctc
     3241 ctccagagtc ataccggcaa acatctcatc catcaggtca atcaacccgg cgttggggcg
     3301 ggttgtgtaa atcccttgtt gcggatgttc tttatcaaca ccacgcagga acagataaat
     3361 aacgccgcca aagtggcgct catagtcgta atcagcaatg cgatggcgca gataacggtg
     3421 cagcgccagg gtataaagct gatattgcag atcatagcgg tgtgcctgca ttgccgctgc
     3481 catagcctgt tgggtgtaag ccgaactatc ttcacccaac cagttggatt tatagtcgag
     3541 caggtaataa cgcccttcgt ggcggaacac caggtcgata aagcctttta acatgccacg
     3601 tgcctgcatg aactccagcg gcgggcagcc agcggatagc gggtcaaact ggcgaattag
     3661 tgcatcaagc tggctggcga tgagcggttc gctaatcggc aggtaaaact ccatctccac
     3721 ctgtttatcg cggtcggaaa gctgactcag gctaacaccc gtttcattga gaggtgcctg
     3781 gaggacagcc gtgatccact cggtcaacac cggttcccac tgcgattcaa agccgccgag
     3841 ttccagtttt tcctgtaccc agttcgggtc aaccggctgg gtaaaattga ggtcttcaaa
     3901 caaactgtgc aagaatgtcc ccggtgacgc accgcgcggg aactgatgcg gtgttaacgt
     3961 cggttcttca acgacgctga ccacgcctgc ggcatcaaca tccagccgtg gcatcagatc
     4021 ctgagcgata ccgtgaccac gctgctgcaa gccggagtag ctggtgacac gccagttatc
     4081 gccgggcaat cgttgtagcg tcctcgcatt cagttctgca gttaaagcat cattaacctg
     4141 ccagggctgg ttatcaccag tttgtgccgt ttgccaggca atatcatcat cgcataacgc
     4201 ttcaatacag gtgcgaagcc ctgccgcatc ttgcggttcc cctttttgca gcaaacgccc
     4261 gagggcactt tggtggacgt cggtgtcacc ttttttatcg ccacgacggc gcaccagcgg
     4321 tgcaacgccg agactgcaat gccaaaccga acgcgtcagc gccacgtaaa gcaaacgcag
     4381 atcttccgcc agacgttcga cctccgcaag atcgacgctt tctggcgcag cgttaagatc
     4441 cagaacagcc tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga cgcggaaatt
     4501 ggtaataaat ggcaaccaga ccagcggata ttccagccct tttgatttat ggatcgtgac
     4561 aatctgtacc agatgtttat cactttcgag gcgcatttgt tggctggagg cattactgtc
     4621 tggctcgagg atatgttgcg ataaccagcg taccagcgca tgttcacttt ccagctgcgt
     4681 tccggcttct tgtagcagtt cgctgatatg caagatatcg gtaagacgcc gctcaccgcc
     4741 tgccgttgcc agcaagtttt ctgcaatgtt acgcgccgac atcagcgccc gcatcatcgg
     4801 cataacgcca cgtttgcgcc agatttgccg ataaccatcg aactcttcga ccaccgcgtc
     4861 ccacgcatgt tcgtcattat tcagcgtttc gatatccagc gcgttcagcc ccatcattga
     4921 cgttgccagc gcactacgca gggtgttctc acgttcgggc gtcatcaccg cctgcaacaa
     4981 ccaaagcatt tcctgcgctt ccagagtttc aaaaacactg tcgcggttcg aaaggtaaac
     5041 ggaagggatt tccagcaacg ttaaggcatc gcgcacctgg gcggcctcct ggcggctgcg
     5101 caccagcaca ctgatgtccg aagcacgcac cggacgcgcg tcgtcgccgt tcatcagcaa
     5161 cgcttcgccc cgctgtccgg cttgtagcca gtcgcgaatt tgcgcagcac ttacctgcgc
     5221 catggtactt tgatagtcgc caacgccgca actttcgcct tccatcagcc acattttcat
     5281 cgcaggctgc gtttcacctt taaagacaaa acgtaacgcc tgatttttcc cggctgattt
     5341 cactggaata aacggtattt cgcgaaacat gaacgcgtca tcagtctggc tgaaaagctt
     5401 gttcacactg ttcaccattc caggtgcgga gcgccagttg gtgtctaaag tgtagtgggc
     5461 gtgaacttca ctacgcgcct tcatataagt gaagatatcc gcaccccgga atgcatatat
     5521 ggcctgcttc gggtcgccaa tcagcaacaa tgcggtttcc ggctgatggt gccagatacg
     5581 gcgaaaaatt cggtactgct gggggtcggt atcctgaaat tcatcgatca ttgccaccgg
     5641 gaatcgcgta cggatcgccg ctgccaacac ctcaccgctt tcgctacgca gcgcggaatc
     5701 gagccgactt aacatgtcat caaaacccaa ttcgccacgg cggcgttttt cacgcgctac
     5761 tgtttcgcgg atctcagcca atgcgcgggt gatcaccaga tcgcggatcg acaatggttc
     5821 tgcaagcagt tgatcgatcg cctcaaacag tggatgtcgc ggggtttccc ccccggcctt
     5881 cgtgcgatct tctaagaaac gctgggagaa tttttccagc gactccggca actgataact
     5941 gtttctctct tcttctgccc aggcgctgat cttgtcgatc catttagcct gattgctacg
     6001 gttaaacttg cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt cacccactgc
     6061 gtcgcgccac tgctgtttta ccgtatcaat acgcgccaca atttgcgcgt gacgggaagc
     6121 cagcgtttca tcatcgggcg gcggtgcttt gataaccggc gcttcacctt gcagataacg
     6181 attaatatcg cgcagcaacg cctgcggccc tttccaggtt tcaaagacga cctgggctat
     6241 ttcacgcggc agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct ggtaacgtag
     6301 cagagactca tcttcaatca gctgctgctc aaacagcatg ccggattcaa aggcattcag
     6361 gttgagcatg cgctggcaaa aaccgtgaat agtaaagact gccgcttcat ccatctgccg
     6421 ttcggctaac aacaaccact tcgcggcttg cgctttatcg tcgatctctt ccagcagacg
     6481 ttcgtacagt ggattgtcgg tggtttcacg cagacaggcg atgcgcaact catggatatt
     6541 gctacggata cgaccacgca attctgccgt ggcagcctcg gtaaaggtga ccaccagcag
     6601 ttcttcaacg gtcagcgggc ggggaaaggc ggcggaaccg cctagtccaa gtaacaggcg
     6661 caaatagagc gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt caatcaggcg
     6721 ctcgccctga aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac tcattctttt
     6781 cactcatcag gggcattgtt tgctgcaacg cgctgacgtt ctcccacact ttccagcctt
     6841 caggatgcac atattcggct ttcccgttct ggctgccgga aatctgcgac agaatagcca
     6901 tgccttgcgg ctcgaccacc gcctgatgga agaaatcagc aagtttttgc ggcgtcagca
     6961 gttttatctg ggccacgatt ttatcacgcg aatcgaagcg catattgccg cgatcgaaat
     7021 ctttacttaa cttcaatgct tcttcgccga gcgtttgcgg tgcctgcagc atctgggtaa
     7081 ttaccgcctg ctggatttgc gcaaactcat ctggcttcat cgctcgcaat tttgcctctg
     7141 cggttgggaa aaacgccttg taacgctccc acaagaatga aggctgttta tcattgcttt
     7201 gcaaaaggaa gcccatgccc cactgacgcc ccacgctcat tggaaacgca aacacggcat
     7261 agcccaattg ttcttcggta cgcaactgat tgtagaacca cggctgtacg atctgcccca
     7321 acagagagct ataggctgag cttgtgtatt catcgtagcc agtcggtaca aataccgctg
     7381 ccagtgcgga gtcggtgctg ttaccggctt tttcaaagat gacggattgt tttttatcga
     7441 ccactacatc tttgtttcga caccactctg aaccatcagc gcccaactgt ttttgcacat
     7501 cgcgtgccag cgttgttgcc tgggcctcgg tcatgttgcc gataaccata aactctggtc
     7561 gagcccctga ttttaaggcg tcacgataag ccagcacctc tttcaacgta atggagggca
     7621 aaattttacg ccgttcatct cgcgagaagt acggcacttg cgagagcatc tgcgcgggca
     7681 taatcgcctg ctcaaacgct ttaccctttt ctgcggaatc catcatctgg ttataccagg
     7741 acttcgcctg ctcaagctga tcttccgtag cggtatagct aaagtatccc tcgagcaatg
     7801 cctggaacag ctgcggcaga cgctgggtgt aaccattagc attaaccata aggccgttgt
     7861 tagcgttggt ggaaaaactt atgccaccaa ccgacgcctg gttgcttaac tgatcaagcg
     7921 ccagccctgc gagataatca ttgagcgcaa acatcacctg attgcgggcg ctgtccatgg
     7981 ctttcggatt acgcaaaatc aggctgacat cagctttggg ttcgctggaa aaataacggc
     8041 ttggcgcata caccacgcgc agattcgact catcaacaat cagctctgga tggtcatatt
     8101 tcttctctga cttaatcagc gagaaatcgt caggaatata ggggttaagc tccggtaagg
     8161 agagcgcaat attggcagct ttttgctgcc agtcagcgaa agtttgttcg ctgattttat
     8221 cgacctgata cggcgcatcg acaaaataag ccgttttatt gtgcggctct ttcgggctga
     8281 tataccagat acgcgcattc tgcggcgtca tcatcgccag acgttctttt actgctttag
     8341 catcgtaccg atcggcaata ttgactgcat ccagcgtatg ctcaacagga acgcgaatca
     8401 tggtatctgc cagccattcg acgtaatcca tatcacgggt gattgacgga taacggaagt
     8461 cgatatccag cacattcgcc agttcatcga agtattgttt atcgatccct ttttcacgta
     8521 acaaattgag gtagctaaaa atagccgcca caacctgatc gcgattcgcc agacctttat
     8581 cggttaaaga cgcagagatc gctaatacgc cgctgttgcc gttgacgata ggatcggagt
     8641 tggcgctaat gccctcaact aacccctgct tttgcagcca gtcagacagt gtacctgggc
     8701 tgcgattacc aatcagatag gtaatcaatt catccgtttt actgcggaac ttcgctgagt
     8761 tgttatcgat gcgaaactca acgcgcagaa ctttacgcgg cagcgctggg acgtaatgaa
     8821 tgataatgcc cttttgcgcg tcggtgacta ccggcacggt gatttccggt tttttgctct
     8881 ctttgttcgg cacgcgacca aaagtgtccg ccgccatttt tgccaactcc ggcagcggtt
     8941 tattactgta aataaccgcc ttcatcaaat tggcggaata gtacttctcg tggaaatctt
     9001 tcagcgcctg ctgcaccgga ttaccaggtt tgtcgcttaa agtttcgagg ttaccaccag
     9061 aaaactttga accggggtgt gccgggttaa tggtttctgc gctgacctgt gccatgcgca
     9121 tcccgtcacg tgtacgcgcc atggttaatt cagcgttcac tgcattacgc tcacgttcgg
     9181 cgtatttctt gtcgagcaag ggttctgcaa tagcatcggc caggcggtct accgcaccgg
     9241 gcaaggcgtc gttctcaact tccagataga aagccgtgcg atacggcgca gtgctggcat
     9301 tgtgactacc gccgtgcatt ttgagatatt cggccagact gtcagcctgc gggtactttt
     9361 tcgaccccat cagactcata tgttcaaggt aatgtgccag cccctggtac gcctcgggat
     9421 cttccagcga cccaacgggc accaccagcg ccgatagcga tttaactgcc tgcggatcag
     9481 aaaccagcaa gaccaccata ccgttatcca gacgtatagc ctgatactgg cggttatctt
     9541 tatcactttt acggatggtt tcctgaatcg gctgccatcc cgtttctgcc tgacttaagg
     9601 gtgcccaaag ggcaactaac aacaataatg ctttgaacca ggtgctgcgg ggcattcacg
     9661 gacctcataa tcaactttat tttctggcca gattcaacag gttaacgcaa atcatctgcc
     9721 agaatttaat cttgtgctgc acgagtcagc ctatgtttat ataaccatca gtccgtgact
     9781 ggtgcgcatc ataaagtaag cggatagatt gcgcaatttt tatacagcgc tcatgactga
     9841 ttaaagcgaa acagcggtaa caggaaacgt tgcgactgtt caacgattgc ctccattgtc
     9901 tctggtgtta attgccgcca gagcctttga taccagatat catcaccttc gccactcacc
     9961 atcatgttgc cttcgtaagc ctgaaggaat ttcgtacggg ctttttgcaa cgtggaatcg
    10021 tcatccagca tggcatcgtt ttgcgcgtca taacaggttt ttagccacgc gccgccactt
    10081 tcaggtaaca ccagcaatgg cgcggacatc ccttcacgat acccctcaat cagttgtgag
    10141 aggtaatgca aagcctgttc ggctgcaagc ggcggaaaac gccactcgcc gtctttgcgt
    10201 agaaaaaggc gactttcacc attaccaccg ctggcacagt agacaaggtg ttccagccaa
    10261 agttgcattc cctgcgccac acttaataaa gagggacgcc agcgcaacaa gccatccggc
    10321 tgcacctgcg gcaaccagcc agttatctgc acaccgttgc aagcgagatc aatttccata
    10381 ctctgccccg gctggcgaca ggcaatgact ctgtcggcaa gctgctgcat ctcctggcac
    10441 tgtgtttccc agaaaatttc accaaaagcg ccatacggta aatcccctgc cgcccggaag
    10501 cggcggaaca agcgttcggc atcatcctgc tcaaccagtg cattcaataa ctgctgattg
    10561 atttgataac ggctaagtcc ttccagaata aatggctcgg tgtcggggat ttcgctgtct
    10621 tcagtacgga agttcacctg caaacgcatc tggaagaacg cccgcaccgg atgtgcccag
    10681 aatcgttgta gcgtttccag cggcacggtt tccggtaagg taaacggcag cggctgaaca
    10741 aattcagaat gtgctttacc agcctggctg gccgcaggta gccattcacg agcatagctt
    10801 tgtcgttcgc ctggctggta gttttgcgga tcaaacggca tccgggtatg gaggcaagta
    10861 agatgcgctt ttacccttgc ctcgctttca tcacagttga gcgcttcatc gcccggtaga
    10921 taatgacttt gcccgatgta gtcgatcagt tcctgcacca gtaccgacgg gaaacgttca
    10981 ctgttatcct gaatggaacg cccgatatag ctgatataga gtttttgctg cgcggaaatt
    11041 aacgcttcca ggaacaggta gcggtcgtca tcgcgacggc tacggtcgcc acgcttcggt
    11101 ttctggctca tcaggtcaaa gcccaatggc gcaagctgac gtggataaac gccgtcgttc
    11161 attcccagca ggcaaaccac tttgaacgga attgaacgca ttggcatcag agtacaaatg
    11221 ttaaccggcc cggcgagaaa acgctggctg atacgttctt gatccaggcg ctgtgccagt
    11281 tcatcacgca atagtgacag cggcaccgca tcgccatact gcgcacctaa accttcggcg
    11341 ataatcgcct gccattgttg ttcgatcagc gtcatcgccg cttcggtttc cgcatccggc
    11401 aggaagaggg cgttgagcat atcgcgacaa accggcaacc actcttccag cggacgctcc
    11461 tgtgccagcc cgcgacgcca gatgtttagc tgcattagca gtgaagccag atgccccacc
    11521 agttctgcaa ttaagccgct cgattcatca taaggtagaa ccgattgcca ctcgccctgc
    11581 gcgctctcca tcgcgtagcc caacaacata cgcgtcaggc caaatcgcca ggtgtgttgt
    11641 ccggtggcgg ggagttccag ctcgcgaacg ttgtcgtcat ctatccccca acgaatgccg
    11701 gattcgttga cccactgacg taaataacgc agcccttctt cggtgatgtc aaaccgcgcc
    11761 gccagcaccg gcacatccag taatgccagc acgtcttccg acacaaagcg gctgtctggc
    11821 aacgataaca ggctgataaa cgcttcaagt acaggatgcg actgccgcgc ccgacggtcg
    11881 gaaatggcgt aaggcaggta acgatccgca ggtgcgctac caaacacagc ctgaataaac
    11941 ggactgtagc tgtcgatatc agccaccatc acgatgatgt cgcgcggagt aagtgtcggg
    12001 tcttcttcca gcatcgccag caggcgatcg tgtaaaactt caacttcacg ctgcgggcta
    12061 tggcaaacgt ggaaggtgat actgctatcc agtggatcaa gcggacgttt gttatcgcta
    12121 cgggaaaact cttcgatgtt cacaccagca acggcgcggt tttccagttc cagaatgtca
    12181 gactgaatgt tatgcagcag gttatctggc gtcacatcga caaaggcgtc cagctcctgg
    12241 ctgctctcca ggtcagaaag gagataaatg tagtcgcgcc caagcttgcc ccatgaggcc
    12301 agcagcgggt tgccgacatc ctgttcacca tcgctgttaa agagctgccc ggcattttcg
    12361 ctgtcgcgaa atagcggtaa ttcgcgatct tcaaaactgt gtcggcgctg gcgagtcagt
    12421 agtttcgcca gataagcagg atctttaatg tcgccccagt aataacggca ggggttggta
    12481 aacaggagat ggatttcaat atgtttaccc agcgcctgga gcgcctggag ataaacaggc
    12541 ggtaacgcgg aaataccgca tataaagacg cgcgaaggta accccggcgg gcaggtcgtc
    12601 gcggactcca gcgtttcgat aaagcgctga tagagattgg cgcggtgcca gcgcggttgt
    12661 ccgagttcat gggtatattc caccagcgcc ttccacaacg gcgcttgcca ggcctgtgcc
    12721 tctcccagcc cttcaaccaa atgtcctgtt tcccactgtg ccagccagtc cggacgatag
    12781 accagatact ggtcaaacag gtccgccgct tttgaggaaa gctggaacag ctttcgcttg
    12841 tcgctatcgt cagtcagata atgccgcaac agggtaaagt cttcgcgctc cagcaattgc
    12901 ggcagcagag tcatcagttt ccagctcatg ctctgtttgt taaaggcgct ctctttgggg
    12961 atctccggta acacccggac gaacatatcc cagataaagc tcgcaggcag cggaaaatca
    13021 atgtttgccg caataccaaa cttttgcgac agggtcattt gcagccactg tgccataccg
    13081 gtactttgca ccagaatcat ctctggttcg aaaggatcgt ccagccgttc gcgttcgaca
    13141 ataaactcca tcaacgcttc cagcacgtcc agacgattgg aatggtagac ccttaacata
    13201 gcggctcctg actactgacg attcgggcaa tgcaggcggg tcatctcgcc tgctctgccc
    13261 ccgggtgaaa ctagcgtaac gctgatgctg acacatcccg cctgcgatgt ctgcattcgg
    13321 ttgacctgcc agttggcagg tggcgaaatc gcgcgcaatt gcgtttgctg ccaaccatgc
    13381 cgccagagct gttggtactg gtttctgctg gtaagactgt tcattaatgt tcgctggaaa
    13441 cccgataacg cagtgacaat catcaccatc aacaccatcg ccaacattac ctccggcagg
    13501 ctaaagcctt gttgattctt cagggaagct gacataacgc cccctctttc agcggacaaa
    13561 aatcgctcca gccgcgtggc gaaaaaacaa tttttccatc aatgacttcg cctgttcgcc
    13621 acaacgaaac gccttcataa ccggcaatta ataaggcttc attatcttcc agtaaacgca
    13681 aacaaacccg ggcaccagtg gcagcgtaca gtaagcactg aactgcgggc tgcgtctgcc
    13741 aggagtgcat tttcccccac gccaacgccg actgaacgat ggcctggcgg cgcagtgact
    13801 gactttccat gctcacgcga gaggcaaaac tgcgatcctg ctggctcatt ccttgtaata
    13861 gcaagctacc caaaaccagc agcatcagga ccagagccag tgacgaaaca cccttttcgc
    13921 ggttcacagg ttgaatcctg tcacgctata gctggcatcc accacggttt gcggttcaga
    13981 cttactggca gcacgcatat taaccgtcaa caccagcgag aagccgctga catcctgacg
    14041 tacgacctga aaagtgtcga taatgatggc atccggatta gtgactttat cccagccctt
    14101 accttcacag gatgtcgcac cgcgtagcgt ttccaacact tgctccttca gacgaaatcc
    14161 aatctggtcg gactctttta ccggttcgcg atcccagata ccgttactgt tcgcatccca
    14221 ctgcacaatg atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagatgcc
    14281 atgacaataa cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc
    14341 atcttccagt gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa
    14401 gcgtgcagcc cccaacaaca atacgctact gatcgccata gcaatcaaca cttccagcag
    14461 agaaaaacct tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt
    14521 cttccccacg gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gccaatatgc
    14581 ccggcccatg cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt
    14641 tcgacttcgg gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattcgcc
    14701 ccagcaacgg aactcacaag gcaccataac gtcccctccc tgataaaact gatactgtgg
    14761 tcgcggttat gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg
    14821 gcggtttgcc aaagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg
    14881 cttagcatga ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt
    14941 gttttcatgc cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct
    15001 tcggcgcatc ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggcg
    15061 tctcgaagaa tttaacggag ggtaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg
    15121 atgtttcgta attagatagc caccggcgct ttaatgcccg gatgtggatc gtagccttca
    15181 atctcaaagt cttcgaaacg gtagtcgaag atggattcgg gtttacgttt gataatcaac
    15241 ttcggcagcg gacgcggttc acggcttaat tgcagatgag tttgatccat atgattactg
    15301 tacaggtgcg tatcgccacc ggtccagaca aaatcaccca cttccagatc gcactgctgc
    15361 gccatcatat gcaccagtaa cgcgtagctg gcaatgttga acggcaggcc gaggaagacg
    15421 tcacaggagc gctgataaag ctggcaagag agtttgccgt ctgccacata gaactggaag
    15481 aatgcatggc acggtgccag cgccatttta tccagttcgc ctacgttcca cgctgaaaca
    15541 ataatgcggc gggaatccgg gtcgtttttc agctggttca gtaccgtagt gatctggtca
    15601 atatgacgac catctggcgt tggccaggcg cgccactgtt taccatacac tggtccgagg
    15661 tcgccgtttt catcggccca ttcgtcccag atggtgacat tgttttcgtg tagataagca
    15721 atgttagtgt cgccctgcag aaaccacagc agttcatgga tgatggaacg caggtggcaa
    15781 cgtttagttg tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa
    15841 atggaaagcg ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc gagcactttt
    15901 tgcatcagtt ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga
    15961 cgatatgccc agaccatcat gatcacaccc gcgacaatca tcgggatgga aagaatttgc
    16021 cccatgctga tgtactgcac ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac
    16081 tcaacaatga tgcgaaacgc gccgtagcca atcaggaaca agccagacac ggctcccatc
    16141 gggcgcggtt tacgaatata caggttgagg ataataaaca gcaccacacc ttccagcagc
    16201 agctcgtaaa gctgtgatgg gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc
    16261 cactgcgggt tggtttgcag cagcaaaata tcttctgtac gggagcctgg gaacagcatg
    16321 gcaaacggga agttcgggtc aacgcggccc cacaattcac cgttaataaa gttgcccaaa
    16381 cgcccggcac caagaccaaa cggaatgagt ggtgcgataa aatcagaaac ctggaagaag
    16441 gaacgtttag tacggcgggc aaagataatc atcaccacga taacgccaat caggccgccg
    16501 tggaaagaca tgccgccgtc ccagacacgg aacagataca gcggatcggc cataaactgc
    16561 gggaaattgt agaacagaac ataaccaata cgtcccccga ggaagacgcc gaggaagccc
    16621 gcatagagta agttttcaac ttcatttttg gtccagccgc tgcccggacg attcgcccgt
    16681 cgtgttgcca gccacattgc aaaaatgaaa cccaccagat acatcaggcc gtaccagtga
    16741 agcgccacgg gtcctattga gaaaatgacc ggatcaaact ccggaaaatg cagatagcta
    16801 ctggtcatct gtcaccacaa gttcttgtta tttcgctgaa agagaacagc gattgaaatg
    16861 cgcgccgcag gtttcaggcg ctccaaaggt gcgaataata gcacaagggg acctggctgg
    16921 ttgccggata ccgttaaaag atatgtatat gatccgcgct ataaccctcc gcgaatcagc
    16981 ccgcccatgc cgcgacgctc cataaaggct gcaacctgat ggcgaacttc ggtcgccagt
    17041 tgcgcttcca gactacgctg cgcaagattt tctgcttcgg cataatcaat gcgccgcagc
    17101 aggtatttta cccgcgctac agaacgtccg ttcatagaca gatggcgata cccaagccca
    17161 atgaggattg ccacgcacat gggatcgccc gccatttcac cgcacaaacg gagatcgatt
    17221 ccatgtattt ccgcttcccg ggcgatcatc gccagagctc gtaacattgc aggatgaaga
    17281 ctgtcataaa tgttcgccac ccgggtattg ttgcgatcaa cggccagaat gtattgagtc
    17341 agatcgttgg tgccaacaga gatgaaatcg acccgctttg ccagatgcgg cagcataaat
    17401 accattgacg gcacttccag catgatgccg atacgtggtt tgggaatttc gtaaccgatc
    17461 atctcctcga cttcacgtcc ggcacgttca atcaggcggc gtgcttcgtc aacttcatcg
    17521 aggcttgtga ccatcggcaa cagaatattc aggttgcccg tagcggcatt agcacgcagc
    17581 atcgcccgca cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc
    17641 caacccaggc atggattctc ttcgctaatc ggcatgtaag gcagctgctt atctgctccg
    17701 acatccagcg tacgcaaggt gacgggttta tcattgaaca tttgcagcat cccctgatac
    17761 tgcgccacct gttcttcttc cgacggaaaa ccactttgca gcatgaatgg gatttcagtg
    17821 cgataaagac cgatgccatc aatacggctg cccagttttt cttcatgttc cgggcttaaa
    17881 ccagcattga gcatgacttt tatacgctca ccgcttttta actgcgcggg taaattgacg
    17941 tcatcttccg ccagacggct aagctcaatc tcttcactaa ttagccgctg atattcttgc
    18001 agcagtaccg gctccggatc gaccagcaat tcaccgcgat agccatcgac gatcagcgtc
    18061 cgacgatgca gcaccgaagg ctgaatatcc gcgcccatca cggtagggat ccccagcgca
    18121 cgtaccatga tcgcagcatg ggagttggct gcgccatctc gcacgacaac accgactaag
    18181 cgatcctggg gcagctcagc aagagttgtc gctgacagtt catctgccac cagaatgaaa
    18241 cgttccggcc aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag caatcgctga
    18301 cccagcgcac gtaaatcgcc agcccgctct ttgagatagt tatcgcttag cgcggcaaac
    18361 tgttcggcaa atttttcaat gaccgttttt accgcccatt ctgccaccga gcctttatca
    18421 acctcggcaa acaattcgcg acgcagccgg gtgtccgaaa gcaggtgcga gtaaagatcg
    18481 aaaatagccg ccgtttcttt ttgtgcaccg gcggcaaagc gtttgctgta gcggcgaaac
    18541 tcgtttgccg cttcttccag cgccccggtc agtcgttcgc gttccagagc cggatccagc
    18601 gttgatgcct gatacacctg ttccattaaa ggtaacgtgg catcctgcca gccttcggca
    18661 atcgctacac caggtgctgc cggtaatgcg cggattcgcg tctggcgata ttgcccaaac
    18721 aaggcagtca actgcgactg agaaagaatc gctgccatct gggtggcaag cgtcaccagg
    18781 aaggattctt cactttcgtc atactggcgc aactctcgtt gctgtaccac cagtacacca
    18841 agcaactggc gacgttgaat aattggtacg cctaaaaacg cgcggaaacg ttcttctttt
    18901 acggagggga tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc
    18961 gccagcctgc caaccaggcc gacgatccct tcatcaaagg cgagcgttac agtgcgaccg
    19021 cgtggttttt tcagcccccg ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc
    19081 aggtagaccg aacagacctc ggtatccatc gcaagacaga tgtcggtaac cagaatattt
    19141 aacgcctcat tcaggcgtgg tgcgctggct accttttcga ctatttcgcg caggcgagtg
    19201 agcataattg gcgtgactta acctctttta cgtcgataag cagatgcgtt ttgtggtttt
    19261 ggcgtatttt cctgcagtga catcaccaca ctcgcgaact ctttcattac cctacggtag
    19321 acatcacgtt taaatgacac cacctgtctg accggatacc agtaacttac ccatcgccag
    19381 ccgtcaaact ctggtgtact gctggtttgc atattgattt ctgcatcgcc gctcaccagc
    19441 tgcaagagaa accatttttg tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc
    19501 aaacgtttcg gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct
    19561 ttgcggctta atcctacttc ttcaaacaat tcacggtaca tcgcctgctc tgcggattct
    19621 ccggggttga ttccgccttg cggaaattgc caggagtgct gaccaaatcg ccgggcccac
    19681 attacctgcc cctggcgatt acaaatcacg atacctacgt ttgggcggta gccatcgtca
    19741 tcaatcaccg gactacctca aaataaagct ttatatacga atgattgttt catactccag
    19801 gaagacggta aaccactctc tgcagggcat tacacactaa caacaattga ataactcaca
    19861 gttatgtgca gagttataaa cagaggaagg ggtggatagc cgtttttatt caccttttct
    19921 gtggatagag ttgtgaagaa ctacggaatt actacgggaa aacccggagc attctgaata
    19981 agcccgatat agacatttta aaatattcat tattagtcaa tgcattacat gtaatttaag
    20041 taatgaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga
    20101 tcaaccaatg ccgtatttat ccacagaatg tgccactaag ttaagcactg aaccactaaa
    20161 aactggagtt tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag gtttttcaca
    20221 gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga
    20281 tttggaaaac ccgttgcagt gttgcgcaac tcgattaccg gcaacctaaa aagcaatata
    20341 aatcagataa ttgaacaacg catgtggaaa aagttacact gcgaatattc ggcacataat
    20401 tgctgtttgt tttttaacca aggtatcatg acatgtccca acctcgccca ctgctctctc
    20461 ctcccgaaac tgaagaacag ttgttagcgc aagcacagca actttctggt tatacattgg
    20521 gagaactggc ggcacttgcc gggctggtta cgccagagaa tttaaaacgc gataagggct
    20581 ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa cctgaacaag
    20641 attttgctgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc
    20701 tggaaacaac attcgtttgt gttgccccgt taacgggcaa tagcggagtg acctgggaaa
    20761 ccagccacgt gcgccacaaa ctcaaacgcg tactgtggat accggttgaa ggcgagcgca
    20821 gcatcccgct ggcgcagcgt cgcgttggat caccgttgct gtggagcccg aatgaagagg
    20881 aagaccggca gctacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcagg
    20941 ttgagcggat taccgctcgt cacggggagt atttacagat acgaccgaaa gcagcgaatg
    21001 cgaaagcgct taccgaagcc attggtgcac ggggcgaacg gattctgacg ctgccgcgcg
    21061 gcttttattt gaagaagaat ttcaccagtg cactactggc ccgtcatttt ctgatccagt
    21121 agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag
    21181 ggctttttag atgttatttg catggataac cgatcctaac gcctggcttg cgctcggtac
    21241 gctgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt
    21301 ggcaaagctc cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat
    21361 ggttatgcgt ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga cgaatccgct
    21421 ttttacaata ttcagtcagg aaatttctgc ccgtgacttg attctgcttc tgggtggctt
    21481 gttccttatc tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg
    21541 gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat
    21601 tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc tgtttattat
    21661 gatggccgcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga
    21721 ttttgtcgag cgccatcctt cggtaaaaat gctggcgctc tctttcctga ttctggtggg
    21781 ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc
    21841 gatgttcttc tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa agaacccgct
    21901 ctgatactcc gtacgctctc ctgcgggagg gcgtactcac ttctgttacc ctcacagttt
    21961 aagattttcc tgctttcaag atatataacg tcagtttata aacagactat tatcataggt
    22021 gggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg
    22081 gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg
    22141 attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat
    22201 cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaataat tgaacgttaa
    22261 caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt cccttccctc
    22321 tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg caatatcacc
    22381 gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg acgtttggtg
    22441 aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct cagggcatta
    22501 accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaacg caagggttaa
    22561 ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag ttaattatcg
    22621 cctccaaagt gagcggaccg tcgcgcaata atgacaaggg catccgcccg gatcaggcgc
    22681 tggatcggaa gaatatccgc gaagcgctgc atgacagcct caagcgtctg cagactgatt
    22741 acctcgatct ttatcaggtg cactggccgc agcgcccaac caactgcttc ggcaaactcg
    22801 gttatagctg gacagattct gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg
    22861 cagagtacca acgagcggga aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg
    22921 gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt gtcaccattc
    22981 agaaccctta cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt
    23041 atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacgctg accgggaaat
    23101 atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg ttcacccgct
    23161 atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg
    23221 gcctggcccc tgctcagatg gcgctcgcgt ttgtacgccg tcaaccgttt gttgccagca
    23281 ctctgctggg cgcaaccacg atggatcagc tgaaaactaa catcgaaagt ttgcatctgg
    23341 agttaagcga agacgtatta gctgaaattg aagcggtgca tcaggtttat acttatccgg
    23401 caccataaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc gctgccagat
    23461 ccacagcgcc gttattgcca gcgcaaatag cgcaccgaag ccaatgccaa tgggcacgac
    23521 cgggatgcct accattaccg ccagcgagta aatgcccagc atcaacaaca tggcgctgtt
    23581 ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt taccccgctc
    23641 ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc aacacgccaa tcagcatcaa
    23701 caaggcatag gctggcagct gctcgtgttg cagggagaaa atcagtacca ccacgccaat
    23761 caaaattccg gctggcatac agcgtgacac ggtttccagc gtaaccagct tcgctgccgc
    23821 acctgcgcca accacgatac caatcgctac catcgcgttg agataggtcg gcgtagcgtt
    23881 atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca gcgtgacacc
    23941 cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat tgcgccatag
    24001 cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg actgccccgg
    24061 acgcgccgcc gccagtttgg gaatgtagat attggcaacg accgcaccac cgtaggccag
    24121 tgcgcatgcg gccagggcga ccaggacatg ccagtcagcc agcacaccac cggctacgga
    24181 accgagcaaa atcgccgcta tggtagaagc ttccattaaa ccgttagctt ttactaactt
    24241 actacccgtg gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg ctgcagcacc
    24301 aacacccacc agcgtatagc cgagaaacgg attgatacca aagcagatac tggctgcgcc
    24361 cagcagcttc aggccgttgg caaacatcat cacccggcct ttggcgaagc tatccgccac
    24421 ctgcccgaca aacggcgcaa aaagaatgta agcacctaca aacaccattt gcaggatggg
    24481 ctggctccac tccggataga actgcgcttt cagtaacgcc agagtggcaa acagtagggc
    24541 attatcgcca aacgcagaga gaaactgcgc cacgataacc gctttcatcc ccttcgacca
    24601 caacgaagtg ttagtgtgca ctgactcact catcgtgttg ttccacttcg tctacccagc
    24661 ttttcaacgt gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt ttcaaatagc
    24721 gaatatcacg cggtacagca agctccggca cgccgtgctc acgggcatac tgttgcaact
    24781 tatcgcgcgt cagttcgtta tctgtggtga aaagcaccag tgcctcgcct ttgctggcat
    24841 cgctcttaat cgcagtggca tggactttat ctggcgaaac accaagtgcc agttgttcca
    24901 ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg cggccctgaa
    24961 tctgcacaaa gccctgctcg tcaaaacgca caatatcgcc agtgtcatac cagccgcgct
    25021 ccatttcgcc gcgaacattc tcggcggtgg gcacttccag tacacctggc ttctccaccc
    25081 gcagatagcc gttcattatg ttcggccctt tcagttgcag gcgtccgccc tcttcgatac
    25141 cagggaccga caacaggcgc gcatccattc ctggtagaat acgccctacc gtaccgggtt
    25201 tcgccgccat cggtacgttg atagaaacga caggcgcgca ttcggtcaca ccgtagcctt
    25261 caaggatgcg caggccaaat ttatcctgcc aaagctgttt ggtactttct tgtagttttt
    25321 ctgcgcctgc caccacatag cgtagacgat agaagtcata cgggttggcg aaacgcgcgt
    25381 agtgaccgag gaaagtcgaa gtgccgaaca acacggtgca actgcggtca tacaccaact
    25441 ccggcacaat acggtaatgc agcgggcttg gataaagaaa cacttctgca cctgtgagca
    25501 gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa cagcggtaac gccgacataa
    25561 agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg ctcgacattc gccagaatgc
    25621 ttttatggct atggacgacg ccttttggat ggccttcaga accggaggta aaaaggatca
    25681 gcgcctcttc ttccggctgc tgtttaacct gtgccagacg cggcatcagc aaatgagcga
    25741 agatccatac tttgtcggca gtggtgacat ctgcttttaa atcttccaga tagacccagc
    25801 gtacctgagt aagttgctcc ggcagatgcc agagtttgcc tttatcgaga aactggcggg
    25861 aagtgaagat ggttttgatt tcagccgccg taatagcact ggtcagccct tttaccccag
    25921 cagtgtagtt catcattgcg ggaatgcggc gacgggcgat ggccccaaaa atcactgccg
    25981 cactgatgcc tgcattgggc agcattaagc cgatgcgttc gccttcaaca ctgtattttt
    26041 caaggatgcg tccaacaaac agcgttttcg taagcaattt gcgataggag tctggggtaa
    26101 aattgacgtc ttcgacacat ttcttccctg ctccgaagcg gtacattgct ctcagtaaag
    26161 attcgtacag cgtttcacgc gggcgcaccg ccattcgcgc ttccatcatt atttgatgca
    26221 gcatttcgcc agcgattttg cgacggtcac gggcacgcgg cgcatccggc atctccacct
    26281 gcgttggtgg caaaatatgc agagtaattt gcgggaacaa gcgacgttta accagacctt
    26341 tcaggcggct gaagtgcgta agttccgccc cttcaatacg cacaggaata accgttgcac
    26401 cagacttcgc cgcgacaaaa cccgcgccat cgtagatttt catcagcgag cctgtggtgg
    26461 tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg ttccaccaga cgtaccagat
    26521 gtttaatcgc cataggttgc gtcgggtcga gaggaacaaa gtcgataaat gatttcagcc
    26581 aacgcatata ccactgttgg cttattgagg tgtaaacggc aaacactgga cgcacaggta
    26641 aaaacagtcc aagcaaaatg ccatcaataa aagagacgtg attaggcgta attagaacgc
    26701 gctcgccctt cagtgcctgg gggtcacccg taacgcgaac gcgatacaaa acacggcaca
    26761 aatttcgaaa aaagctaaaa agcattccaa ctccctttgc tctgattcag taaaagcgaa
    26821 tggagggaga ttacacgaga taaagaacgc gagcgacagt aaattaggtg cgaaaaaaaa
    26881 cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac cgaataatct
    26941 caccaatcaa tacctctggg atcttgattg tggtctgcac gacgactctt cgccagcgag
    27001 aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta aattgtcggt tactgttaca
    27061 gattgatgac cggcaaaaaa aaacctgcgc atctgcgcag gctggtgtaa ttcatgtgct
    27121 caacccgaag ttgacttcac ctatcaatac ctctgggatc accactttag caacctgaag
    27181 ccaaacgcca ccagcggtca atcgcaacag cgtttcgcaa agtgtaacca aaggtttgca
    27241 atctcttttt ttgtcttact tatttgcgtt tggctcacat tcccacgatg aaaacacgcc
    27301 gccccttgaa ccaacgggcg ttttccgtaa cactgaaaca atgtaagcgt ttacccacta
    27361 aggtattttc atggcgacca taaaggatgt agcccgactg gcaggcgttt cagtcgccac
    27421 cgtttcccgc gtcattaata attcacccaa agccagcgaa gcttcccggc tggctgtgca
    27481 tagtgcaatg gagtctctta gctatcaccc gaacgccaac gcccgtgcgc tggcgcagca
    27541 gaccactgaa acggtcggtc tggtcgttgg tgatgtttcc gatccgtttt tcggtgcaat
    27601 ggtgaaagcg gtcgaacagg tggcttatca caccggtaat ttcttattga ttggcaatgg
    27661 ttaccacaac gaacaaaaag agcgtcaggc cattgagcaa ctgatccgcc atcgctgtgc
    27721 tgcgttggtc gtccatgcca aaatgatccc ggatgccgat ttagcctcat tattgaaaca
    27781 aatgcccggt atggtgctga tcaaccgtat cctgcctggc tttgaaaacc gttgtattgc
    27841 tctggacgat cgttacggtg cctggctggc tacgcgtcat ttaattcagc aaggtcatac
    27901 ccgcattggt tatctgtgct ctaaccactc tatttctgac gccgaagatc gtctgcaagg
    27961 gtattacgat gcccttgctg aaagtggtat tgcggccaat gaccggctgg tgacatttgg
    28021 cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag cttttgggac gaggaagaaa
    28081 tttcactgcg gtagcctgtt ataacgattc aatggcggcg ggtgcgatgg gcgttctcaa
    28141 tgataatggt attgatgtac cgggtgagat ttcgttaatt ggctttgatg atgtgctggt
    28201 gtcacgctat gtgcgtccgc gcctgaccac cgtgcgttac ccaatcgtga cgatggcgac
    28261 ccaggctgcc gaactggctt tggcgctggc ggataatcgc cctctcccgg aaatcactaa
    28321 tgtctttagt ccgacgctgg tacgtcgtca ttcagtgtca actccgtcgc tggaggcaag
    28381 tcatcatgca accagcgact aaccgcagtt aaagcaattc cagcgccagt aattcttcga
    28441 tggtctggcg acggcgaatc aaccgcgcct gaccattatc aaacagaact tctggtaaca
    28501 gcggacggct attgtagttg gatgacattg atgcgccata tgcccctgta tcatgcagta
    28561 ccagataatc acctgccttc acttccggca aggcgcgggg ttcaacattt cccccttcct
    28621 gctgggtaaa gacatcgccc gattcacata acggtccggc gacgacggtt tccaccgttg
    28681 gtgcgtgttc cagagaacga ccatcagctg ccagggcact gatatggtgg taactaccgt
    28741 acattgccgg gcgcatcaga tcgttaaacc cggcatcaac cagcacaaag tggcggctac
    28801 ccatttgttt gacgctccgc acctgagtaa ttaacacgcc agactgcgct accaggaagc
    28861 gacccggttc aatttccagt ttcacagggt ggcccaaatg gcgggcgatt tgctcacgcg
    28921 cggcattcca cagaccataa taatgttcgg tatcaaccgc ctcttcaccc tgttgataag
    28981 gaatagaaag cccaccgccc gcagaaatag cctgtaaatc ctgaccgaat tcgatgacct
    29041 gacgcaccat agcaccacac acctgttcca gatgggcata atcaacgcca gaaccaatgt
    29101 gcatgtgaat gccgaccagc tgcagatgat gacgttgtat cacgtccagt gcggcgggca
    29161 gatcggtgta ccagataccg tgcttgctgt tttcgccacc ggtattggtt ttttggctat
    29221 gtccgtgacc aaaccccgga ttaacgcgca gccatacccg atgccctggc gaaacctggc
    29281 ccagttggtc gaacatatca acagaacccg cattcaccgg aatttgcaat tcactgacgc
    29341 gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac aatatcatcg gggtgcgttt
    29401 gcggattgta acccgccgcc aacgcacgct ctatttcgcc taacgagacg gaatccactt
    29461 tcacgccctg ctcacgcatt aagcgcaaaa tatgaatatt ggaacaggct ttctgtgcaa
    29521 agcgcaccac atcaaactgt ttcagcgctg caatctgccg acgaataatt tgcgcatcgt
    29581 agacccacac cgggcagcca aattcagcgg gcaaacgcag cagattttcg gcggtgagat
    29641 cggtatcggt gctgaacagt gaatgtggca taacaaactc cagatgagtg cttttttatg
    29701 attacgccac atcataaaaa gaataaaaaa tatcgattta tgtcgagtct atgcaaaaat
    29761 gatatggatt accggattgc gagagagcgc taatggccgc cgttaactta cgtcatattg
    29821 aaatttttca tgcggtaatg accgccggaa gcctgactga ggcagcacac ctgctacaca
    29881 cctcacagcc aaccgtcagc cgcgaactgg cgcgctttga gaaggtgatc gggctgaaat
    29941 tgtttgagcg cgtacgtgga cgattacatc ctaccgtgca aggactgcgt ctgtttgaag
    30001 aagtgcaacg atcctggtac ggactggatc gcattgtcag cgccgcagaa agtctgcgcg
    30061 agtttcgcca gggagaactg tctattgcct gcctgccggt cttttcgcaa tcttttttac
    30121 cgcagctcct gcaacccttt ctggtacgtt atcccgatgt cagcttaaat atcgtgcccc
    30181 aggaatcacc gctacttgaa gagtggctct cggcccagcg tcatgattta ggactcactg
    30241 agacactcca tacgcctgcg ggaacagaac gtaccgaatt actctcttta gatgaattgt
    30301 gtgtgttacc tccgggtcat ccgctggcgg taaaaaaggt attaacgccg gatgattttc
    30361 agggtgagaa ctacatcagc ctttcccgta ctgacagcta tcgccagttg ctggatcagc
    30421 tatttactga acatcaggtt aaacgacgca tgatcgtaga aacccacagc gccgcgtcag
    30481 tctgcgcaat ggtacgggcg ggggtaggtg tttcggtggt taacccgctc accgcactgg
    30541 attatgcggc aagcggttta gtggtgcggc ggttcagtat tgcggttccg ttcaccgtca
    30601 gcctgatccg ccccctgcac cgcccgtcat cagcgctggt gcaggcgttt agtgagcatt
    30661 tacaagcggg gttaccgaaa ctggtcactt ctcttgacgc tattttgtcg tcagctacga
    30721 cagcataaaa gcgacagcat cctcggcatg gatcgccgca gtatcaaaca caggcaaaac
    30781 actgcgctct tctggcacca gtaaaccaat ttctgtgcag ccaaaaatga cgccctgtgc
    30841 gccctgttct gcaaggcgag caatcacttg cgcataataa gcgcgtgacg cttcggtaaa
    30901 ttgccccaga cacagttctt caaaaataat ctgattaatt ttcgcccgtt catccgcttc
    30961 aggaataaga caattgatgg aaaattgttc cgtcagccgc ccgcgataaa aatcctgttc
    31021 catggtgtaa cgcgtaccca gcagcgccac acgagtcatt ccggccccgg taattgcacg
    31081 tccggtggca tccgcaatgt gtaagaaagg cagagtgcaa cgtgactcaa tggcatccgc
    31141 cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc gctgtaagcc
    31201 aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac gctggcactc
    31261 ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa gcccaccaag
    31321 ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt cccagctcat
    31381 tcctcccagc aaaccaattg ttttcattcc tctctcctga tgtgtgttac cgcaacaagg
    31441 ctgaataagg aacgagacaa acgcctcaac ggccaggtgc ccgatctcta ttaacgaaaa
    31501 aagggccgga tgtacagcac atccggcccg tgaaatcaga cgccgatatt tctcaacttc
    31561 tcgcctgcca tcagtttgcg ttcgatatgt tccagcgtga catttttggt ttccggaatg
    31621 agccagaaag tgatgcccac aaacgcaatg ttcagcgcag tgtagagcca gaacgtaccg
    31681 gcagcgccaa tgctatcaag cagtgtcagg aaggtcgcgc cgataatcat attcgacacc
    31741 cagtttgtcg tcgtcgaaca ggtaataccg aaatcgcggc atttcagcgg ctgaatttca
    31801 gagcacagga tccacaccac tggcgcggcg ctcatcgcat aaccggcaat acacatcatc
    31861 gtcatgccaa cagagagcca ggacaagcca ctggaagccg taccgttatc aaactgcatc
    31921 aggcaatagc ccagcaccag agtgcctaac gccatcacgc tgaaaccaat tttcagagcc
    31981 ggtttacgac ctgctttatc taccgtaaag accgcaatga aggtcgcgaa cataaaggtc
    32041 agtcccacga ccagagtcgc aatcatctgt tgttctgtgg tcgtaaagcc cgccattttg
    32101 aagatacgcg gcgcatagta catgatgatg ttcataccgg taaactgctg catcgcctgc
    32161 aacaacatac cgagaaacac agcacgacgg acgttacggt tgatcttaaa cagtgcccaa
    32221 ccgccttgtt ttaacttcag gctttcacga atttcgttga gttcttctcg cgctttttcc
    32281 gacgtatcgc gcagcatacg caacacttct tccgcctcaa tatgacgccc cttttccgcc
    32341 agccagcgcg ggctatttgg caggaagacc accagaataa tcagcaggac tgctggtaaa
    32401 gcaagaaccc ccaacattgc gcgccagtta ccgctataac tgaacgctgt atcggataaa
    32461 aacgccagca cgatgccgag tgtgaccatc aactggtaca tactgatcat cttaccgcga
    32521 acgttttcac ttgccatttc agaaagatac agaggagcgg tgtaagacgc gatcccgaca
    32581 gcaatgccca gcaccacacg agcggcgatt aacatctcta cgctggtcgc aaaagcggac
    32641 cctatagaac tgagtacaaa caggatggcc cccgccatca ggctgtattt acgccccagg
    32701 cggaacgaca gccaaccatt aaacagcgca ccaattgctg cgccgagcat catgctacta
    32761 accacccatt cctgcaaacg actggtcagc acaaagtgat cggtaatgaa cggcaacgct
    32821 ccggcgatta cgccgatatc aagaccaaat aacaatcctg cgaccgcagc agctaccgaa
    32881 acaaacatat tcatacgccg cgtatcacgc aaagaacgtg gcgttaaagc agattccgta
    32941 ttgatagtaa ccattttttc ctgccagcag agagtaagac atagtgaaaa aatacgtgaa
    33001 caattcacac aggtgtcagg tcggaaacag cataaatatg gattaaattg ctgcgacatg
    33061 ccgttatgtg atggatattc caattt
//
LOCUS       DABSYT020000044        33011 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.044, whole genome shotgun sequence.
ACCESSION   DABSYT020000044 DABSYT020000000
VERSION     DABSYT020000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.044"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..315)
                     /locus_tag="ID725_RS12525"
                     /old_locus_tag="pgaptmp_002505"
     CDS             complement(<1..315)
                     /locus_tag="ID725_RS12525"
                     /old_locus_tag="pgaptmp_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851086.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLL"
     gene            838..2766
                     /gene="thrS"
                     /locus_tag="ID725_RS12530"
                     /old_locus_tag="pgaptmp_002506"
     CDS             838..2766
                     /gene="thrS"
                     /locus_tag="ID725_RS12530"
                     /old_locus_tag="pgaptmp_002506"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGV7851087.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2770..3312
                     /gene="infC"
                     /locus_tag="ID725_RS12535"
                     /old_locus_tag="pgaptmp_002507"
     CDS             2770..3312
                     /gene="infC"
                     /locus_tag="ID725_RS12535"
                     /old_locus_tag="pgaptmp_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGV7851088.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3409..3606
                     /gene="rpmI"
                     /locus_tag="ID725_RS12540"
                     /old_locus_tag="pgaptmp_002508"
     CDS             3409..3606
                     /gene="rpmI"
                     /locus_tag="ID725_RS12540"
                     /old_locus_tag="pgaptmp_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGV7851089.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3659..4015
                     /gene="rplT"
                     /locus_tag="ID725_RS12545"
                     /old_locus_tag="pgaptmp_002509"
     CDS             3659..4015
                     /gene="rplT"
                     /locus_tag="ID725_RS12545"
                     /old_locus_tag="pgaptmp_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGV7851090.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4132..4256
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4138..4182
                     /gene="pheM"
                     /locus_tag="ID725_RS12550"
                     /old_locus_tag="pgaptmp_002510"
     CDS             4138..4182
                     /gene="pheM"
                     /locus_tag="ID725_RS12550"
                     /old_locus_tag="pgaptmp_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGV7851091.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4465..5448
                     /gene="pheS"
                     /locus_tag="ID725_RS12555"
                     /old_locus_tag="pgaptmp_002511"
     CDS             4465..5448
                     /gene="pheS"
                     /locus_tag="ID725_RS12555"
                     /old_locus_tag="pgaptmp_002511"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGV7851092.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5463..7850
                     /gene="pheT"
                     /locus_tag="ID725_RS12560"
                     /old_locus_tag="pgaptmp_002512"
     CDS             5463..7850
                     /gene="pheT"
                     /locus_tag="ID725_RS12560"
                     /old_locus_tag="pgaptmp_002512"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGV7851093.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7855..8154
                     /gene="ihfA"
                     /locus_tag="ID725_RS12565"
                     /old_locus_tag="pgaptmp_002513"
     CDS             7855..8154
                     /gene="ihfA"
                     /locus_tag="ID725_RS12565"
                     /old_locus_tag="pgaptmp_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGV7851094.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8255..9235
                     /gene="btuC"
                     /locus_tag="ID725_RS12570"
                     /old_locus_tag="pgaptmp_002514"
     CDS             8255..9235
                     /gene="btuC"
                     /locus_tag="ID725_RS12570"
                     /old_locus_tag="pgaptmp_002514"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGV7851095.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9298..9849
                     /gene="btuE"
                     /locus_tag="ID725_RS12575"
                     /old_locus_tag="pgaptmp_002515"
     CDS             9298..9849
                     /gene="btuE"
                     /locus_tag="ID725_RS12575"
                     /old_locus_tag="pgaptmp_002515"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGV7851096.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9849..10598
                     /gene="btuD"
                     /locus_tag="ID725_RS12580"
                     /old_locus_tag="pgaptmp_002516"
     CDS             9849..10598
                     /gene="btuD"
                     /locus_tag="ID725_RS12580"
                     /old_locus_tag="pgaptmp_002516"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGV7851097.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10676..11140
                     /gene="nlpC"
                     /locus_tag="ID725_RS12585"
                     /old_locus_tag="pgaptmp_002517"
     CDS             10676..11140
                     /gene="nlpC"
                     /locus_tag="ID725_RS12585"
                     /old_locus_tag="pgaptmp_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGV7851098.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11387..12100
                     /gene="ydiV"
                     /locus_tag="ID725_RS12590"
                     /old_locus_tag="pgaptmp_002518"
     CDS             11387..12100
                     /gene="ydiV"
                     /locus_tag="ID725_RS12590"
                     /old_locus_tag="pgaptmp_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGV7851099.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12163..13599
                     /gene="selO"
                     /locus_tag="ID725_RS12595"
                     /old_locus_tag="pgaptmp_002519"
     CDS             12163..13599
                     /gene="selO"
                     /locus_tag="ID725_RS12595"
                     /old_locus_tag="pgaptmp_002519"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGV7851100.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13603..13794)
                     /gene="hemP"
                     /locus_tag="ID725_RS12600"
                     /old_locus_tag="pgaptmp_002520"
     CDS             complement(13603..13794)
                     /gene="hemP"
                     /locus_tag="ID725_RS12600"
                     /old_locus_tag="pgaptmp_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGV7851101.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13926..14972)
                     /gene="aroH"
                     /locus_tag="ID725_RS12605"
                     /old_locus_tag="pgaptmp_002521"
     CDS             complement(13926..14972)
                     /gene="aroH"
                     /locus_tag="ID725_RS12605"
                     /old_locus_tag="pgaptmp_002521"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGV7851102.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15129..15962)
                     /gene="ppsR"
                     /locus_tag="ID725_RS12610"
                     /old_locus_tag="pgaptmp_002522"
     CDS             complement(15129..15962)
                     /gene="ppsR"
                     /locus_tag="ID725_RS12610"
                     /old_locus_tag="pgaptmp_002522"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGV7851103.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16295..18673
                     /gene="ppsA"
                     /locus_tag="ID725_RS12615"
                     /old_locus_tag="pgaptmp_002523"
     CDS             16295..18673
                     /gene="ppsA"
                     /locus_tag="ID725_RS12615"
                     /old_locus_tag="pgaptmp_002523"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGV7851104.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18730..20375)
                     /gene="fadK"
                     /locus_tag="ID725_RS12620"
                     /old_locus_tag="pgaptmp_002524"
                     /pseudo
     CDS             complement(18730..20375)
                     /gene="fadK"
                     /locus_tag="ID725_RS12620"
                     /old_locus_tag="pgaptmp_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20432..20725)
                     /gene="ydiT"
                     /locus_tag="ID725_RS12625"
                     /old_locus_tag="pgaptmp_002525"
     CDS             complement(20432..20725)
                     /gene="ydiT"
                     /locus_tag="ID725_RS12625"
                     /old_locus_tag="pgaptmp_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGV7851105.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20722..22011)
                     /gene="ydiS"
                     /locus_tag="ID725_RS12630"
                     /old_locus_tag="pgaptmp_002526"
     CDS             complement(20722..22011)
                     /gene="ydiS"
                     /locus_tag="ID725_RS12630"
                     /old_locus_tag="pgaptmp_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGV7851106.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22067..23005)
                     /gene="ydiR"
                     /locus_tag="ID725_RS12635"
                     /old_locus_tag="pgaptmp_002527"
     CDS             complement(22067..23005)
                     /gene="ydiR"
                     /locus_tag="ID725_RS12635"
                     /old_locus_tag="pgaptmp_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGV7851107.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23025..23789)
                     /gene="ydiQ"
                     /locus_tag="ID725_RS12640"
                     /old_locus_tag="pgaptmp_002528"
     CDS             complement(23025..23789)
                     /gene="ydiQ"
                     /locus_tag="ID725_RS12640"
                     /old_locus_tag="pgaptmp_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGV7851108.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24105..25016
                     /gene="ydiP"
                     /locus_tag="ID725_RS12645"
                     /old_locus_tag="pgaptmp_002529"
     CDS             24105..25016
                     /gene="ydiP"
                     /locus_tag="ID725_RS12645"
                     /old_locus_tag="pgaptmp_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7851109.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(25059..26210)
                     /gene="ydiO"
                     /locus_tag="ID725_RS12650"
                     /old_locus_tag="pgaptmp_002530"
     CDS             complement(25059..26210)
                     /gene="ydiO"
                     /locus_tag="ID725_RS12650"
                     /old_locus_tag="pgaptmp_002530"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGV7851110.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(26224..27819)
                     /gene="ydiF"
                     /locus_tag="ID725_RS12655"
                     /old_locus_tag="pgaptmp_002531"
     CDS             complement(26224..27819)
                     /gene="ydiF"
                     /locus_tag="ID725_RS12655"
                     /old_locus_tag="pgaptmp_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGV7851111.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(27964..28722)
                     /gene="aroD"
                     /locus_tag="ID725_RS12660"
                     /old_locus_tag="pgaptmp_002532"
     CDS             complement(27964..28722)
                     /gene="aroD"
                     /locus_tag="ID725_RS12660"
                     /old_locus_tag="pgaptmp_002532"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGV7851112.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(28753..29619)
                     /gene="ydiB"
                     /locus_tag="ID725_RS12665"
                     /old_locus_tag="pgaptmp_002533"
     CDS             complement(28753..29619)
                     /gene="ydiB"
                     /locus_tag="ID725_RS12665"
                     /old_locus_tag="pgaptmp_002533"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGV7851113.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(29631..30896)
                     /gene="ydiN"
                     /locus_tag="ID725_RS12670"
                     /old_locus_tag="pgaptmp_002534"
     CDS             complement(29631..30896)
                     /gene="ydiN"
                     /locus_tag="ID725_RS12670"
                     /old_locus_tag="pgaptmp_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7851114.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(31123..32337)
                     /gene="ydiM"
                     /locus_tag="ID725_RS12675"
                     /old_locus_tag="pgaptmp_002535"
     CDS             complement(31123..32337)
                     /gene="ydiM"
                     /locus_tag="ID725_RS12675"
                     /old_locus_tag="pgaptmp_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7851115.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(32437..32793)
                     /gene="ydiL"
                     /locus_tag="ID725_RS12680"
                     /old_locus_tag="pgaptmp_002536"
     CDS             complement(32437..32793)
                     /gene="ydiL"
                     /locus_tag="ID725_RS12680"
                     /old_locus_tag="pgaptmp_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGV7851116.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(32929..>33011)
                     /gene="rprA"
                     /locus_tag="ID725_RS12685"
                     /old_locus_tag="pgaptmp_002537"
     ncRNA           complement(32929..>33011)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ID725_RS12685"
                     /old_locus_tag="pgaptmp_002537"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         8399 a         7919 c         8382 g         8311 t
ORIGIN      
        1 taataaggaa atgatttctt ttttatagtg taatgtaatg aatatatttt ctgcatggat
       61 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      121 tcgacagttt gcggtaaatt cctgagtaac acagtatgac agcatcgaac ttaagtcaga
      181 ggtatcaagt agggtgatga gttggggcgc aaattgatta ataggctctg atgaaggcgt
      241 tggtatgtcg ctatcgatgt gcattctatg aggagactta taatgagttc tgataataat
      301 gtcgttttga ctcataatct attcgcctta tatatgtctg gaggttttga gtatgatgat
      361 taattattat cgccagaacg aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt
      421 tatcattgtg atatgaccac ttcgtcatta atcttatgat cgtttaaata gtaccgccaa
      481 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaattcgct cgcagtaaac
      541 ctttcagacg ctccgtgatg ttatcagttg ttctttaagc gttttgctgg tgtactcact
      601 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      661 tgttaacatc gctcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      721 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      781 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      841 cctgttataa ctcttcctga tggcagtcaa cgccattacg accacgctgt aagccccatg
      901 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
      961 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1021 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcac
     1081 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1141 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1201 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1261 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtctc cattcttgac
     1321 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1381 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1441 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1501 gcgtgggcag acaaaaaagc gcttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1561 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1621 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1681 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1741 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacgtct
     1801 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1861 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1921 caccgtaacg agccgtcagg ttctctgcat ggcctgatgc gcgtgcgtgg attcacccag
     1981 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2041 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2101 actcgtcctg aaaagcgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2161 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2221 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2281 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2341 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggaacgtttc
     2401 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2461 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2521 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2581 atccgcgagc acactttgcg tcgcgtcccg tatatgctgg tctgtggtga taaagaggtg
     2641 gaatcaggca aaattgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2701 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2761 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2821 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2881 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     2941 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3001 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3061 ttccgtcctg gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3121 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3181 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagtg
     3241 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3301 aagaaacagt aaggccttca agtagcactg tctgtggagc cttcgggctt cacaggcttt
     3361 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3421 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3481 aagcacgcta acctgcgtca cattctgacc aaaaaagcga ccaaacgtaa acgtcacctg
     3541 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3601 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3661 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3721 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3781 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3841 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3901 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     3961 cgacaaagta gcgttcaccg ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4021 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4081 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4141 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4201 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4261 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggatac
     4321 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4381 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4441 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4501 gcggccatta gccaggcgtc agatgttgcc gcgttagaca atgtgcgcgt cgaatatttg
     4561 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4621 cgtccggcag ccggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4681 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4741 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4801 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4861 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4921 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     4981 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5041 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5101 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5161 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgaa
     5221 ccttctgcag aagtggatgt catgggtaaa aacggtaaat ggctggaagt actgggctgc
     5281 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5341 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5401 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5461 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgatg
     5521 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5581 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5641 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5701 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc gaccattggt gctgttctgc
     5761 cgggtgattt caaaattaaa gcggcgaaac tgcgcggcga accgtctgaa gggatgctgt
     5821 gctccttttc tgagctggga atttctgacg accataacgg cattatcgaa ctgcctgcag
     5881 atgcgccgat tggcactgac atccgcgaat acctgaaact cgatgacaac accatcgaaa
     5941 tcagcgtaac gccaaaccgt gccgactgtt taggtatcat tggtgttgcg cgtgacgttg
     6001 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6061 acgacacgct gccgattaca gtcgaagcgc cggaagcctg cccgcgttat cttggccgtg
     6121 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6181 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgtg ctgctcgaat
     6241 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6301 tggcgaaaga gggcgaaacg ctggtgctgc tcgacggtac tgaagcgaag ctgaatgctg
     6361 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc ttcggtggcg
     6421 aacactctgg cgtgaatgac gaaacacaaa acgtgctgct ggaatgcgct ttctttagcc
     6481 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6541 atgagcgtgg cgttgatccg gcactgcagt acaaagcgat ggaacgtgcg acccgtctgc
     6601 tgattgacat ctgcggtggt gaggctggtc cggtaattga tatcaccaac gaagcaacgc
     6661 tgccgaagcg tgcaaccatc actttacgtc gtagcaaact ggatcgcctg atcggccatc
     6721 atattgcgga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6781 gcaaagacga gtggcaggca gttgcgccga gctggcgttt cgacatggag attgaagaag
     6841 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6901 aggcaagcct gattatgggt actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     6961 cgctgctcaa cgataaaggc tatcaggaag tgatcaccta tagcttcgtt gatccgaaag
     7021 tgcagcagat gatccatcca ggcgttgaag ccttactgct gccaagcccg atctctgttg
     7081 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7141 agaaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7201 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7261 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7321 atcttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7381 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7441 gttttgttgg ggttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7501 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7561 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7621 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7681 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7741 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7801 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7861 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7921 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     7981 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8041 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8101 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8161 ctaactaaaa aggccgctct gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8221 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8281 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8341 aagcctttgc gccggtgaac aatggatctc gccaggtgac tggttttctc ctcgtggcga
     8401 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8461 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8521 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8581 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggggcgc ttatcatcac
     8641 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8701 cgttgcatta gggattatct gtagcgcact aatgacgtgg gctatctact tttccacctc
     8761 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8821 gcaaagctgg ctgatgctgg cattgatccc catgttgttg tggatctgtt gtcagtccag
     8881 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccctgtg
     8941 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtagcgct
     9001 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9061 aaccgatcat cgcgcattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9121 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9181 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9241 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacccagg agaaacgatg
     9301 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9361 aagtacgccg gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9421 caatatgagc agttggagaa tattcagaaa gcctgggccg atcgaggttt tgtggtgctg
     9481 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9541 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9601 gaaggacgcc atccgctgta tcaaaaattg atttccgcag cgccgaccgc agtcgcgccg
     9661 gaagagagcg gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct gtaccctgat
     9721 gatattttat ggaattttga gaaattttta gttggcaggg acggattggt catccagcgt
     9781 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9841 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9901 cgctttctgg cgaggttcgg gctggggaga tcctgcacct ggtggggccg aatggcgcgg
     9961 gtaagagtac cttactggcg cgaatggccg gaatgaccag cggtaaggga agcattcagt
    10021 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgtgcctatc
    10081 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10141 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10201 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10261 ctgcggtggt gctgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10321 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10381 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacattgc
    10441 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10501 aagatgtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10561 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cttgaaaatc
    10621 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10681 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10741 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10801 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg gtgtggactg
    10861 ttcgggattt gtggttgtga cgatgcgcga tcgtttcgat ttgcagctgc cccgagaaac
    10921 caaagaacaa gcctctatcg gcacgcaaat tgataaagac gagttgctgc ctggtgacct
    10981 ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt acgataccaa
    11041 caaccaattt atccacgcct ctaccagcaa gggagtgatg cgttcctcac ttgataatgt
    11101 ctattggcag aaaaatttct ggcaggcgag acgaatctag tatgtcgaaa attggacgtc
    11161 gatgatacat cgccgtccat ttactccaga atacagtagt attttgcaga caaaaattcc
    11221 gtaaatggat tattcgttta taatcaggat aatctaaaaa aaggacccct gaaaaagtgg
    11281 ttaacatgga acaaatgaaa gttactcctt ctgtaattgt tatgtcttat ggcctcatcc
    11341 cttttagccg gatactgaaa aacatccttc gagagggacg gttaccatga agattttttt
    11401 ggagaatctt tatcattctg attgctattt cctgccgata agagataacc agcaagtcct
    11461 tgtcggcgtt gagttaatta cccatttctc cagtgaagat ggcacggtgc gtatcccgac
    11521 gagtcgggtt atagcgcaac ttaccgagga gcaacattgg caattatttt cagagcagtt
    11581 ggaattacta aaatcatgcc aacatttttt tattcaacat aaactctttg cctggcttaa
    11641 tttaacgcca caagtggcta cattattatt agagagggat aattacgcag gcgaactatt
    11701 aaaatatccg tttattgaac tgttgattaa tgagaattac ccgcatctca atgaaggaaa
    11761 agataatcgc ggtcttttat ccttatcgca agtgtatccc cttgtgttag gtaatttggg
    11821 agcaggcaac agcacaatga aagcggtttt tgatggtctt ttcacccgcg taatgctgga
    11881 taaaagtttt attcagcaac aaatcacgca tcgctccttc gaaccgttta ttcgtgcgat
    11941 tcaggcgcaa atttcccctt gttgcaattg catcatcgcg gggggcattg atacgccaga
    12001 aatattagca cagattaccc ccttcgattt ccatgctctc cagggatgcc tgtggcctgc
    12061 cgttcccata aatcagataa cgacgttggt tcagcgataa ccctcctgtt tgctggtgtt
    12121 taagacgaga gtaaccgtct acactatcaa acaggaggct ctatgaccct gtcttttatt
    12181 acccgctggc gcgatgaatt gccagaaacc tatacagcac tttcccctac gcctttaaat
    12241 aatgcccggc tgatttggca taataccgaa ctcgctaaca cgctgagtat tccatcgtcg
    12301 ctgtttaaaa atggcgcagg tgtctggggc ggcgaaaccc tactgcctgg tatgtcacca
    12361 ctggcgcagg tttacagtgg tcatcagttc ggcgtctggg cgggccaact gggtgatggg
    12421 cgcggcattt tacttggcga acaactgctt gctgatggca ctacaatgga ctggcatctg
    12481 aaaggtgctg gcctgacgcc ttattcgcga atgggtgatg gacgggcggt attacgttcg
    12541 acgatacgag aaagtctcgc cagtgaggcg atgcattatc tgggcattcc gacgacccgc
    12601 gcgttaagta tcgtcaccag cgattcgcca gtgtatcggg aaacggtgga gccaggcgcg
    12661 atgctgatgc gtgtggcacc aagtcatctg cgctttggtc atttcgaaca tttttactat
    12721 cgccgcgagc cggataaggt tcgtcagttg gctgactttg ccattcgtca ttactggtca
    12781 catcttgaag atgatgagga caaataccgt ctctggttta acgatgttgt cgcacgtacc
    12841 gcatcgttaa ttgcccaatg gcagacggtc ggctttgcac atggggtgat gaataccgac
    12901 aacatgtcgc tgctgggact gacgcttgat tacgggccgt ttggttttct taatgattac
    12961 gaacccggtt ttatttgtaa tcactcggat catcaaggac gttacagctt tgacaatcaa
    13021 cctgctgtcg cgttgtggat tttacagcgt ctggcgcaga cattgtcacc atttgttgcc
    13081 gtagatgccc tgaatgaggc cctggacagc tatcagcagg ttttgttgac gcattatgga
    13141 caacggatgc ggcagaaact gggctttatg acggagcaaa aagaggataa cgcgctactg
    13201 aatgaattat tcagtctgat ggcgcgagag cgcagcgatt atacccgcac atttcgcatg
    13261 ctgagtctga ccgagcagca cagcgcggcg tcaccgctac gtgatgagtt tattgatcgt
    13321 gcggcatttg atgactggtt tgcccgttat cgagggcgtt tgcaacaaga cgaggttagc
    13381 gatagtgagc gtcagcaact gatgcaaagc gttaaccccg ctctggtatt gcgcaactgg
    13441 ttggcgcaac gggcgattga agcggcagaa aagggtgata tgacggaatt gcaccgcctg
    13501 catgaggcgt tgcgaaatcc tttcagcgac agagatgatg actatgtcag tcgtccacct
    13561 gactggggta aacggctgga agtcagctgc tcgagttaaa ggctacttgg tcaacagcag
    13621 cttgccagcc tgagttttac ggagcagata ttcttgcccg tcatgatcga taatcagttt
    13681 gccatccgga cctaacagtg tctggctgga aattcgccga ataggctgcg gggaagcggt
    13741 cttgtgattg gcatccgttt caggcgtgag ttttctgcta tccgtataac gcataaaaat
    13801 taataataat tgatataaca atgattctta ttatcaatat tatggctgac cagagcaagc
    13861 agtaatgtgt tgagaagaca acaagttgcg cagaaatgcc catcccatca ggaatgggca
    13921 cgcattcaga agcgggtatc taccgcagag gctagttttt cgaccaggcg ttcggtatct
    13981 tcccagccca gacacgggtc ggtaatggat tgaccgtaag tgagcggctg accgccgacg
    14041 attttttgcg ttccttcgcg caggaaactt tccgccataa ttccagcaat cgccgtagag
    14101 ccattgcgga tttgctgaca aatatcctca caaacttcta actggcgacg gtgctgcttc
    14161 tggcagttac cgtggctgaa atccaccacc agatgttcag gtaaatcaaa ctcgtgcagc
    14221 gtatcgcagg ctgcggcgat atcatcggca tgataattcg gttttttgcc gccacgcata
    14281 ataatgtggc catacgggtt gccgctggtc tgatagatgg tcatctgacc atttttgtct
    14341 ggcgagagga acatatggct ggcgcgggct gcgcggatag catccacagc aatccgcgta
    14401 ttgccatcgg taccattttt aaaacctacc ggacaggaga gtgccgaagc catttcacgg
    14461 tggatctgac tttcggtagt acgtgcgcca atcgcgcccc aactgattaa atcagcaata
    14521 aactgaccgg tcaccatatc gaggaactca gtcgcggttg ggacgcccag ctcatttacc
    14581 tgtaaaagta atttgcgcgc cagctccaga ccgtgattta tccgatagct gccgtttaaa
    14641 tctggatcgg agattagtcc tttccagccg acaacagttc gtggtttttc aaaataggtg
    14701 cgcattacga tttccagccg tgactggtac tggttgcgca gcgactgcag acgggtggcg
    14761 tactccattg cagcggtgag atcgtggatc gagcaggggc caataatgac caacagtcgc
    14821 ttatcttcac cattgagtat tttttcaatt ctgcggcggg agtcggtgac atgggtggcg
    14881 acgccaggcg ttacgggata ccgtagcgcg agttcggcgg gcgttaccag gctctcaata
    14941 cgcgcagtac ggagttcgtc agttctgttc attacaagtc tcagaaattt gttgcttcta
    15001 cggcaggact gccgggaagt gatgcgcatc acgataaacc aatcgctgct gatttcaagt
    15061 gcgaggtgtg tcacacctcg ccgggtggtt agcatgataa caaaaaaata agctaacgca
    15121 ctagttctct agtacattcg gcgactaagg cccatgatat cgaggatctt ggtggcaatc
    15181 tcttctaccg aataattggt actgttaatc cacgggatct gatttttacg gtacaaggct
    15241 tccacttccg cgacttccat cctgcactga cgaagcgagg catagcgact gttctcccga
    15301 cgttcctcgc gaatcgccgc cagacgttcc gggtcgatag tcaggccgaa caatttatgc
    15361 tgaagcggtt tgagcgacgc gggtagcacc agattatcca tatcgtcggc aataaagggg
    15421 tagtttgccg cgcggatacc aaattgcatt gccagataca gactggtggg ggttttacca
    15481 cagcgagaaa caccgagcag gatcacctga gcctgatcca gattgcgcaa cgaaatgccg
    15541 tcatcgtggg cgagggtgta atcaatcgcc gcaatgcgcg catcatattt attgagatta
    15601 ttagggttaa ggccatgggt acgatgagca atcggcgttg gatccagttt catctcttgt
    15661 tgtagcgggg caaccagcgc ctgaacgata tcctggcaaa aaccttcact ttgcaagatg
    15721 atggcgcgaa tttccggcaa cacgatggag tagaagacca gcggacgcac gcctgtctgg
    15781 tgataaattg cgtcaatctg atccttcact gcccgtgcac ggctctcatt ttcgacaaac
    15841 ggcagcgtga tgctgctgat agtgacggga aattgtgaca ttactgcgtg tcctaatacc
    15901 tccgcagtta ttgccgtacc atcagaaata taaaaaacgt ggcgatcaac agcattatcc
    15961 attttgttct tcccgtgatg cagacataat tctatgaaag cataaattaa aaacgcacag
    16021 aagcgtagaa cgttatgtct ggtttataaa atgaaccttc aattttattt tttatgaaaa
    16081 cagcatttca tttttatggt ttcgtttata ccgatggttt atgtggaaat tgtcgaaaag
    16141 agcagatttg cgcaacgctg ggatcagtct taacaagtaa aaaaatatat ttgcttgaac
    16201 gattcaccgt ttttttcatc cggttaaata tgcaaagata aatgcgcaga aatgtgtttc
    16261 tcaaaccgtt catttatcac aaaaggattg ttcgatgtcc aacaatggct cgtcaccgct
    16321 ggtgctttgg tataaccaac tcggcatgaa ggatgtagac agggttgggg gcaaaaatgc
    16381 ctccctgggt gaaatgatta ctaacctttc cggaatgggt gtttccgttc cgaatggttt
    16441 cgccacaacc gccgacgcgt ttaaccagtt tctggaccaa agcggcgtaa accagcgcat
    16501 ttatgaactg ctggataaaa cggatattga cgatgttact cagcttgcga aagcgggcgc
    16561 gcaaatccgc cagtggatta tcgacactcc cttccagcct gagctggaaa acgccatccg
    16621 cgaagcctat gcacagcttt ccgccgatga cgaaaacgcc tcttttgcgg tgcgctcctc
    16681 cgccaccgca gaagatatgc cggacgcttc ttttgccggt cagcaggaaa ccttcctcaa
    16741 cgttcagggt tttgacgccg ttctcgtggc agtgaaacat gtatttgctt ctctgtttaa
    16801 cgatcgcgcc atctcttatc gtgtgcacca gggttacgat caccgtggtg tggcgctctc
    16861 cgccggtgtt caacggatgg tgcgctctga cctcgcatct tctggcgtga tgttctccat
    16921 tgataccgaa tccggctttg accaggtggt gtttatcact tccgcatggg gccttggtga
    16981 gatggtcgtg cagggtgcgg ttaacccgga tgagttttac gtgcataaac cgacactggc
    17041 ggcgaatcgc ccggctatcg tgcgccgcac catggggtcg aaaaaaatcc gcatggttta
    17101 cgcgccgacc caggagcatg gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg
    17161 tgacatcttc tcgctgacca acgaagaagt gcaggaactg gcaaaacagg ccgtacaaat
    17221 tgagaaacac tacggtcgcc cgatggatat tgagtgggcg aaagatggcc acactggtaa
    17281 actgttcatt gtgcaggcgc gtccggaaac cgtgcgctca cgcggtcagg tcatggagcg
    17341 ttatacgctg cattcacagg gtaagattat cgccgaaggc cgtgctatcg gtcatcgcat
    17401 cggtgcgggt ccggtgaaag tcatccatga tatcagcgaa atgaaccgca tcgaacctgg
    17461 tgacgtgctg gtcactgaca tgaccgaccc ggactgggaa ccgatcatga agaaagcatc
    17521 tgccatcgtc accaaccgtg gcggtcgtac ctgtcacgcg gcgatcatcg ctcgtgaact
    17581 gggcattccg gcggtagtgg gctgtggtga tgcaacagaa cggatgaaag acggtgagaa
    17641 cgtcactgtt tcttgtgccg aaggtgatac cggttacgtc tatgcggagt tgctggaatt
    17701 tagcgtgaaa agctccagcg tagaaacgat gccggatctg ccgttgaaag tgatgatgaa
    17761 cgtcggtaac ccggaccgtg ctttcgactt cgcctgcctg ccgaacgaag gcgtgggcct
    17821 tgcacgtctg gaatttatca tcaaccgtat gattggcgtc cacccacgcg cactgcttga
    17881 gtttgacgat caggaaccgc agttgcaaaa cgaaatccgc gagatgatga aaggttttga
    17941 ttctccgcgt gaattttacg ttggtcgtct gactgaaggg atcgcgacgc tgggtgccgc
    18001 gttttatccg aagcgcgtca ttgtccgtct ctctgatttt aaatcgaacg aatatgccaa
    18061 cctggtcggt ggtgagcgtt atgagccaga tgaagagaac ccgatgctcg gcttccgtgg
    18121 cgcgggacgc tatgtttccg acagcttccg cgactgtttc gcgctggagt gtgaagcagt
    18181 gaaacgtgtg cgcaacgaca tggggctgac caacgttgag atcatgatcc cgttcgtgcg
    18241 taccgtagat caggcgaaag cggtggttga ggaactggcg cgtcaggggc tgaagcgtgg
    18301 tgagaacggg ctgaaaatca tcatgatgtg tgaaatcccg tccaacgcct tgctggccga
    18361 gcagttcctc gaatatttcg acggcttctc aattggctca aacgacatga cgcagctggc
    18421 gctcggtctg gatcgtgact ccggcgtggt gtctgaactg ttcgatgagc gcaacgatgc
    18481 ggtgaaagca ctgctgtcga tggcgattcg tgccgcgaag aaacagggca aatatgtcgg
    18541 gatttgcggt cagggtccgt ccgaccacga agactttgcc gcatggttga tggaagaggg
    18601 gatcgatagc ctgtctctga acccggacac cgtggtgcaa acctggttaa gcctggctga
    18661 actgaagaaa taaaataaat ccccggcggc gttttgtcgc cgggtttatg tgatccccga
    18721 agatgaaact tattcaatct cttcacagac atcctgcgtt aatcgccgca taatatcttt
    18781 ccgtaacaaa aacttttgta ttttacctga ggcagtgcgc ggtagttttt cgattaccac
    18841 gatatgttca ggatatttat attttgcgac ccgtttacgg ctaaaaaagc caccacctct
    18901 tccagcgata atgaatgatg cggcgctttc agcacgacat aagcgcatga tcgttcacct
    18961 aaacgttcat cgggcattgc aaccacacag gcatcgtgaa ttttaggatg ctgcaataaa
    19021 atatcttcca cttcacggct gctaatattt tcgccgccgc ggacaataat atcttttttg
    19081 cgtccggtaa tttttatata gccagcctca tccatacggc agagatcgcc gctgtaatac
    19141 cagccttctt catccagggc acgggcggtt aattcaggtt catcaaaata tcccataaac
    19201 acattggggc cacgcgaggc ttcttcacct tcgcaacctg gcggtaaggt cttgcgtgcg
    19261 tcatcgacca ctttaatctc tacacctgcg gcagcgtaac catcggtgtg cataaagcgc
    19321 gacaaaggat catcgagatt caccaccgca tgcggcgaac tttctgtgga accataaaca
    19381 cttaataatt taatgccgcg ctgctggcat tcacgcgcca cttttttggg gattgtggta
    19441 ccgccgcaaa gaaagaagcg cagcgctgaa aggtctgcgg gttgtttctc tagtaaattc
    19501 aaaagatcat agacaaacgg cgttgcgccg agcatacagg agcaacgctg ctgctcaagc
    19561 agcgcgagac acgcatcagg agtgaaaata tctaacaaca cgctgcgagc gccaattaag
    19621 aatggtgccg ttacgccatg cagaaagccc gttgcgtgac caagtggcgc aggcatcata
    19681 aagacatcct gccaggttag attcagccgc gcgcaataag cccgctcact ggcgagaata
    19741 ttgttatgcg taagcatcac gccctttggc agaccctcgg ttccggaggt aaacagcacc
    19801 gcagctaatt catcgccgtg ggtcgttatc gccgtggtca gtggggtatt gtcggcgata
    19861 atctgactta atgagaggga agaggtggcg ggagccagtt tgtccacgcc gacaatttgt
    19921 tgtagttgtg gaagctgatt ttgcagcggc aggattaaat ctaccggacg cgtttgttta
    19981 aacaacgtcg gtgtaaagaa catttttgcc tgacacttat taagcaccca taccagttct
    20041 gcttcccgcc aggaaggcaa cagcggtacg gaaaccgcac cgattttcag gcaggcaaga
    20101 tagataacgg taaattcaca ccagcctggc agttgaaatg ctatgcgatc gcctgattca
    20161 atacccttcg ctaacatcca gtttgccaga cagctcgcgg cgtgatcgag cgcgctatag
    20221 gtgtacgatg ctccatgatt atcgaccacg gcaattttgt ctggcatcgc acgagcggtc
    20281 tgctgccagt aatcggccag cgaagcatcg ccccatagcc cttgctgacg atacgccgca
    20341 cgacgttgtt cgttaaacgt taatgtcact ttcattttgg actctcgaaa cagaacatca
    20401 ggcccgagat gcgggcctgt gggatgcatg attagccgta gcgaaaatca ataccgaagg
    20461 tgcctgcggg atattgccac tgttcgagaa tagtgttacc gcacagcacc cgacaggtgc
    20521 cgcactccag acaaccggcg gaatcaaaat gaatgtttcc tgcgtcatcc tgcttataaa
    20581 gtccggcagg gcaggctttc attaatttac ggaattcatt gatatcggga tttgccgcca
    20641 aaatgatatg cgggtggccc tcatcaacat ggaatttatt gacgcctaat ttgatgtcga
    20701 cgttaaccgt agcgttctgg ctcatagcgc ggttgctccc ttaatgccat ctttcagcaa
    20761 gttgatcagc ccaattttct tcgcgtgtcc catgatcatt ttgcgtaccg gctggttagg
    20821 cttaccgtca atggtgaaca tctcgttcat gatgtcggct accattcgtg ggtattggct
    20881 aaacaggcgc gggttttcca tcagcgccgg gatcttgcga aaatgctgca tatcacgcat
    20941 gacgcagctt tgttccagct cacgtttgta ttgcgccaga ctgctggcgg agaaatccgc
    21001 gcgttctttg gcggcgatca ctgttgtggc ggcagcctga gccgatgcaa tggctaaatc
    21061 catgccgcgg accgtaaaac ccaaattcag gcagaagcct gcggcgtcac caacgatcat
    21121 cacgccatcg ttaaccagtt gcggtaccat cgccagaccg ccttctggca ccatatgcgc
    21181 ggaatattca agcagtttgc cgccgctaat cagcgggcga atggcggggt gttgtttaaa
    21241 atcttccagc atttgcggca cgcttttttg cgcatgggcg atatcagcca atccacaaac
    21301 cagccccaag gatatggaat ccttgttggt atagagaaat cccccgccca tcaggccgtc
    21361 agaaggggaa ccggcaaaca gccaggcggc accttcctcg cccgtaatat taaagcgatc
    21421 gttgatctgt tctggtgtga ggccaataac ctctttaaca ccaacagcgt aatgatgcgg
    21481 atcggaagcg ggaaccattc ccagcgagcg gccaagcatc gagttaacgc catcggctaa
    21541 aatcaccaca ttggcttcga gaatatcatc cccggcctgg actccagtga ccttgtttcc
    21601 ttcacgaacc aacgcatcga cgcgaactcc cgggataaac tgtgcgcccg cctgctcggc
    21661 ttgttccatc aaccacgggt ccagacgatt acgcaatacg gtataagatg cgtgttgcgg
    21721 aacatctggt tgctcgcggt gaaaatcgag ggtaacggcg ctctcttcgg ttaagaagga
    21781 gattttctcg cgtgtgacct tgcgttctac cggcgctgat gctgcaaagc ctggaatgat
    21841 tgcttcaagt gtgtgggcat aaagacgccc gccggtcatg tttttacatc cggcactgtc
    21901 gccgcgttct atcaccagga tatccagccc ggctcgcgcc atgaccagtg cggcaacgct
    21961 accagcaacg cccgcaccga ccacaatggc atcaaatttg tcatccgaca tgcgttatgc
    22021 tcccttcccg ctgcgtttac agcgggagat gtggttgaaa ggggaattag cggctcaact
    22081 ggctaatcag ggcagggacg actttgtaga tatcgcccac cagaccgtag tcggcatagt
    22141 tgaagattgg cgcattttta tctttattga tggcgacaat cacttttgcg ccgttgccgc
    22201 caaccatatg ctggatctgc ccggagatcc ctagcgtcag gtagagatcg gatttcagca
    22261 acacgccgga gacaccgata taacgttcac gctccatcca gttctcgcct tcggcaattg
    22321 gacgtgaaca gccgacttca gcattcagca ccgccgccag ttcgtggacc attttcaggt
    22381 catcctgcgc tgccagacca cgaccgacgc caaccacacg ttttgctttg ctcaggtcca
    22441 cgctgcttgc ggctttcgca cggcgttcgc gacagagaat ttcatgacgc ggagcaacgt
    22501 aaggtaccgt ttctgtcggg cactgatgag aggtatcact ggtgcacggt tcctgaacac
    22561 cgggtgcaag ggtaatgatc gccagcgagc tgttgatctt ttcctgagcg aacgctaacc
    22621 cgccatacat ccggtgttcg gcgcaaatgt gaccatcgac aatatccacc gccgtggcat
    22681 cgttcaccag cgccgcattc agttgcacac ttaaccgtgc tgccagcgct ttaccacgtt
    22741 tcgtcgcggc cagtagcaac atagcggggt gtttatcttt cagcagagca gcaatgcttt
    22801 cggcgtaatt ttcagtgcgt tgcagcgcgt cgttttgctc aagaacataa atacattttg
    22861 gaccataagg cataactgcc tgcgcctggt cggtattttg tacaatggca tacacctgtt
    22921 ggccccattg ctgagcgccg ccaaacagtt cagcataacg ttcaggatta tcgctaaaga
    22981 cccagacgct gtttaattga ctcatcgttt cttctccgta ggcttcagtt cagggctttc
    23041 ttcagatgtt ccgccagctc ggcaatggcc tccggcgaat cgttatcgag aatgatgtgc
    23101 ttacgttctg tttgcggcgg tacgcgaatg ccggtcagtt cagcaagtgg tgcgctctgg
    23161 ctccagccaa tatcacttgc ctgccactga tttaccggtt ttttacccgc gccgagaatg
    23221 gctttcatcg aaggaatgcg tggcacgtta atatcggagg tgacgcagag cacggctgga
    23281 acagagagtt caataacttc aacgtcatct tcaagcgtgc gttcaatcac cagtgtattg
    23341 ccctgacgct gaatagcact cactgcatta atcaccggaa gttgcagaat ttctccgacc
    23401 agcaagccaa cctgctgggc ataaaggtcg ccggaacctt taccaaagat cagtaaatcg
    23461 aagccgatct tttcaactgc tgccgccagc gcttttgcgg tatcgagagg cagtgcatgc
    23521 tcaagttgtg catcctgcac catatacagg ctgtgcggcc cgcgggatag cacgtctttg
    23581 cgcactttcg agttctgcaa caatgagccg ccaacggtca gcgcggctat ctcatcgtca
    23641 tctgttgcaa gctggcttgc agcttcaatg gcattgagat cgaactggct gattttggcg
    23701 tcggcattgt cgaaattcag ggtgtattct ggagtgacaa caatgtcctg ttcttcaggc
    23761 accagcttaa agcaggttat tattttcatg atttctcctg tgagtcgcat acggaaccat
    23821 ctccgatgga aggtaaaaat ggcctgaaaa tagtgtgcga tagcggtgcg gggttttcga
    23881 cggagcaagc tgttttcctt gccaacaatc ttgcaaaaaa aatgcgatcg ctaaaacaaa
    23941 agtgcaattt gcggcaatat agttattcca tgaccgtaca atcttgcatt cagtgtaaat
    24001 aatcttgttt agctctcgct aaacgcggtt gtataaccgc aatacacgcc gtattttatc
    24061 cggcatatat tgtttttgct tgctcatgga atagggaggt tgtcatgtat caacgctgtt
    24121 ttgataatgc cagtgaaacg ctgtttgtcg ctggtaaaac gccacggctt tcacgttttg
    24181 catttagcga tgatccaaaa tgggagtctg gacatcacgt tcatgacaat gaaaccgagc
    24241 tgatttacgt caagaaaggg gtggcaaggt ttaccatcga ttcttcgtta tatgtcgcgc
    24301 atgcagatga tatagtggtg atagaacgcg gcaggctgca tgcggtggcc tctgacgtta
    24361 acgatccggc aacgacgtgt acttgtgcgc tgtacggctt tcagtttcag ggggctgagg
    24421 aaaatcatct actgcaaccg cattcttgtc cggtaattgc cgcggggcag ggaaaagaag
    24481 tcattaaaac cttatttaat gagctaagtg tgattttgcc gcaaagtaaa aatagccaaa
    24541 catcttcgtt atgggatgca tttgcctata cattagcaat tctttactac gaaaacttta
    24601 aaaatgctta tcgttcggag caggggtata ttaaaaaaga tgttctgata aaagatattc
    24661 ttttctatct gaataataat tatcgcgaaa aaatcacttt agaacagtta tcgaaaaaat
    24721 ttcgtgccag cgtcagttat atttgccatg aatttaccaa agagtatcgt atttccccta
    24781 ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg gtcactcact aatactgaat
    24841 tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa tgtcgatcac tttgccaaac
    24901 tgtttttgcg ccatgtcggc tgttcgccca gcgattaccg caggcaattt aaaaactgtt
    24961 ttgcggaaca agaaatccta tctgaatttc ctcaaccggt aagtcttgcc ggataaatct
    25021 ggcggcgcag tttgttaaga agctgcgccg cctgcagatt atttgttctg atagtctttc
    25081 aggatctgcc gacctgctac gtaaatcata atttcgtctg taccgccgcc aatacgttca
    25141 caacggacat cacgccagaa gcgggagacg cgcgcctcat cggtatagcc cagaccgccc
    25201 atgatttgaa tcgcatcatc aatgacttcc attgcggtac gtgcacagta cagttttgcc
    25261 agcgccgcgc tggtgcgcag tgattgatgc tgatcggctt gccatgccac tttcagcacc
    25321 atgttgcgca tgttgtcaat cttaatcgcc atcagcgcca gtttttcctg gatcatctgg
    25381 ttatgaccaa tgggtttacc aaaagcgata cgctggttgg catagcgagc ggcatcttca
    25441 aaggcacact cggcaaagcc ggtgctgcgc gcggcgttga tcaggcgctc catctcaaag
    25501 ttgtacatca cattgaggaa acccattcct tcttcgccca ccatgtcgct ctcttcaact
    25561 tccacgttgt cgagatagac ttcgcaggtg ctgagcatat gccagccgat tttatgcagc
    25621 gggttaatct taatgccggg cttactggag tcgacccacc acagggtgaa ggcttttttg
    25681 ggatctttcg gttgcggatc gcgcgccagc accagcatat acgggtactc tttcgcaccg
    25741 gtaataaagg ttttctgtcc gttgatgtaa accttgccgt ttttacgcgt gtaagtggta
    25801 gtggcactgt tgttatctga gccagcacct ggttccgtca acgccagggc ataggcggga
    25861 tcacccgttt ccagggtgct ttctgccgtt ttacgtagct gctctgcaga accgaaacgg
    25921 cgcatactgt gaatacattg accgttggta attaaaaatg ccggagcacc gcattttgac
    25981 acttccatca gcgccagcat ttgggtgacg taatcagcag ggataccacc aaattcttcc
    26041 ggcacgccaa gcatggaaat accgttatcc gccagtgccc gcataaactc acgcggatat
    26101 gtcccgtttt gatcgcaggt gcggaaatac tcttccggaa agttagtcgt aatcagttcg
    26161 cgaatactgg ccagcagcag ttcttgttct tcagttaaag aaaaatccat cgtgatactc
    26221 ctgttaatga gccgcttcag gcaggacaaa acccatcgcc gcatcgataa ataatctttc
    26281 gtccatcagt ttgagttctg gcgaaatcac tggggtgaaa tccattttgt cgagaatatc
    26341 tttttgtaaa tcgacgccag gggcgatttc gattaaatgc agtccgtctt ctttcagcgt
    26401 gaatacggcg cgctcagtga tataacgaac atccagccct cgctcgagag cgatttttcc
    26461 gctaaaagta atttccggta gttcccgaat aaatttcttc acccgtcctt cctggacgat
    26521 attcaatttg ccatcggcaa tttctgtttt taaactgccc gcagttaacg tgccgcagaa
    26581 aataattttc ttcgaggtgg cactgatatc aataaatcca ccggtaccca tgattttgcc
    26641 attgaattta tgcacgccga cgttaccgtg ctggtcgact tcagcaaaac tcaaataaca
    26701 aacatccaga ccaccaccgt gataaaaatc aaactgggac gtcatatcca gaatggcacg
    26761 ggtattcacg ttcgcgccaa aggcgatacc ctgtgaagta attccaccaa tcggacctgt
    26821 ttctaccgtc agaataaagt catcagcaca accttcttcc cgggcgacca ggccaatgcc
    26881 gtcagcaata ccgacgccga cattccccac cgcgccttta cgcatttcga ataatgcacg
    26941 ccgtgcaact aatttacgtt ggtttagggg caggctaagt ttggtgctgt catcaagggt
    27001 gaagtcacca gaaataaagc ggttaaccgg cgcaccgcca tatagttggc tttgatccgg
    27061 atcgaccacc acaatatcca ccagataacc cggaatacgg acagatttag gatgcagtgt
    27121 ggctttctta accattttct gcacctgcat catcacaata ccgccgttat tgtgcaccgc
    27181 ctgggcaata accaatgcgt cgagatacat cacctcatct tcaaaagtgg cgtagccttc
    27241 actgtcgcag gtggtagcgc gaatgaaagc aatatctggc gcaatcgctt tgtaatagag
    27301 atattctttg ttatcaaact cgaccagttt aatcaggtct tctttagtga cttcattcag
    27361 tttgccgcct tgctggcgtg gatcgacaaa tgtcccgatg ccaatatcac taataatacc
    27421 aggctggtgg gctgcggcgg cgcgtaaggt ttgtgtaagt acaccttgtg gatagttata
    27481 agcaataatt ttattttgtt ctgcgagatc agaaatacgc ggcgattgtc cccagtgacc
    27541 gcataatgcc catttcacca gaccttcttg cgccagcgga ctaataccgc ggtcggcgcg
    27601 atcgccaagc cctgttggac taataatcga taaattacgt ggcgtttgag tctgtttata
    27661 tttatcagca agagcagtaa ttaacgtggt ggcttccaga ataccgccgc cagcgcctaa
    27721 cacacaaagt gttgcttcgt cgggaatata attcaccgct tcctgtgccg acaggaccgg
    27781 cactcgtcca ttaatacgag gtggttttac aggtttcatt gttaattcct gattcaggtg
    27841 tgattttccc tacgccagat ggataattag cgcacagaga ctcacgaaat atttttatta
    27901 aatacggttg aatatatttt tttagttcgg cgggggagga tattcccgcc gaaatattat
    27961 tgcttatgcc tgatgtaaaa tagttaatac tgtgcgcaaa tcatttaccg agatttgccc
    28021 tggcgcagag gcttttttta ccgcaccaaa agttgccgcc gagccaaata cttcaccagc
    28081 cagacgagaa attacgccag tttttgccat tgacatcgtg atgattggac gatcggcata
    28141 ctgctcctgc atctccaggg tcgcggcaag caacgccagc acatcgctgg tactttgcgg
    28201 catcagcgca atcttaggaa tatcggcgtc gaaggactgc attttgcgca gacgggcaat
    28261 gatttcttcg gcttccggcg ttttatggaa gtcatggttg gacatgacaa ctttcacatc
    28321 atgcgcgtgg gcgtaggcga cggtttcttt aacctgatca tcgccggtaa ataactccag
    28381 atcgatcata tcaaccaggc cgctgtcgat ggctgcacga ttgagagcaa tataagcctc
    28441 ggtggaaatc gcctgctcgc cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt
    28501 ttctggcatg gtttcacgga gaatttttgc cgccgccatg acagactcca cattggagag
    28561 gtcggcaaag tggtccacac gccattccag aatatcaaag tccgcttcac gataggcgag
    28621 agcttcggat ttcactcggg cgatatcttt cgccatcagc gagacgatga ttttaggtgc
    28681 gcccgtacca atgacgagat cttttacagt tacggttttc attttttacc ctttctgcac
    28741 gcggtcagcc tgtcaggcac cgaaccccat gacctgttta acatattcca gagggaaatc
    28801 tttgccagtc cataacgtga actgttcagc cccttgccac aacaacatgc cgtatccatc
    28861 aatcgttttg caaccagctt gttgcgcctg ctgcaataac ttcgtcatat gcgggttata
    28921 cacgcattca gtgaccagca gtcccggatg taacagactg atatcattaa ccaatgattt
    28981 attctcaagg ggtttcatac ccacttttgt gccattggtt aaaatgtcgg cggaagccag
    29041 ggcttcagca aaggcttgct gatcggcgag atcggtaacc gtgacgacac aatcggtgtt
    29101 ttcgttaacc cgctgcgcga aggcgagggc tttatcgaag aacttatccc gacggttaaa
    29161 gagtttaatt tcttttaaac cttcaattgc cccctgcgcg ccaatcgccg ttgaggcacc
    29221 tccggcccct aacagcacca ttgttttgcc tttgatatca aaaccgctct ctttaatggc
    29281 gcgaatatgg cccgtgccgt cggtgttata gccgcgcagg tagccatcat cattaacgat
    29341 ggtgttgatg gcacccacca gtttggctgc tggcgttaat tcatcaacat attcacacgc
    29401 cagttgtttg ttcggcattg atacaccagt tccgcgcatt tttagagctt ttaatccttc
    29461 aattgctccg ggaaagctat cgttatccac ttcgaaggcc atataggtaa atggcaatcc
    29521 cgctttttct aaggctttat tctgcatttc aggcgataaa ctgtggcgga taggataagc
    29581 catcaaccca atcaattcgt attttgcggt aacatccata attaactcct ttaacctcta
    29641 tgcttaattg tgtttaaccg ggtgctaaat ttgcgctcgc caaaccgcac atcattctca
    29701 ggaataataa aaacgcggta atagcggata aaaacaataa ttgcggtaat cagggccagc
    29761 agcgcgaaag taaaatcgag aacaatgata tattgcaggc cgatgttcga cagataaccg
    29821 gtaatcagcg gaataacaaa gttagccagt ccacccatca tcatataaat actggtgact
    29881 ttggctttgc tttttgggaa aaactctgac atgaccgaaa caccgagctg taaaatgccg
    29941 ccagctgctg agaaaccgat aacaaaggcg ccggcattac acaccagtgg agaagggtac
    30001 aggtagataa tggctgctgt tattgttgcc agtgcagagt taaatacatt agcccagatg
    30061 ggccggacca tttttttcag tagtgcggca aaaataaaga cacagaccag cgagcccata
    30121 ctgtaataag agatggtttt taatgcctca gcttctgaca taccagcaaa agccatcgca
    30181 tatttgggca tccacaccac aatcacataa aaggtcgaga atgcggctac accgaacagt
    30241 accgatgaaa caccttccag ccagactaac ggtttgctgt tcatttgcgg taattcatta
    30301 gctacgctgg cgtccaccaa ctggctgggg aatttgcttt tcaacagcat cagcgtgatc
    30361 agtacaaaca gaatacccgg aataatcagc ccatagccgt accagatatt attgagcaac
    30421 atatagctca ccagcattgg gtagaacatt tgcccaaatg acaccatcgc tttaaccagt
    30481 atgaccgccg aaccagaggc tttcggaaag cattccatga gcgcggggta gccacccgta
    30541 tccagcgctg agttagcgat acctacgcac actgccagac cgtaggcgag agttaaattc
    30601 gggcaagcgg gaataccaaa gaagaatagc agatacatta ttactgccat taatatcacc
    30661 gcccgacgac caaacttatc ggagatcaca ccgaagaata aaatactgat caatcgcccc
    30721 aaaccgatac cggaaattaa gtaggcaatg cccgcgttgt cagtggaaaa cttttccgcc
    30781 agagatgaca tattttgggc aagcgtaata acactaatgc cgtgcaggaa gtagctgaag
    30841 taaatacaaa gaacagccag gataaatggc gtgctgaaag ccttattttg agacattttc
    30901 acagctcctt gctgaaagat aatttcgtta ccgtacgtat tgttctccga cggtttacat
    30961 aattactata ctgaagatga ttttccctgg gtacatggca taaagtttat gtttccagga
    31021 atattgcatg tcatagtttg tttttgatcg cgattttcct tagttagcgc agaaagagtt
    31081 atttatccgt aagatcttgc atgacctgaa aagaatattg cattacccac ccggagcgac
    31141 attttccttt agcgagtgat gccgcgtttt tttgcggctg cgtaagccga taaacagtgc
    31201 cagtaaaaaa ccgatggcag cgatggcggt atcgaaccac ataatatcgg caatacttct
    31261 ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc ccgcactgta
    31321 atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta acgtcaggcc
    31381 aatttgcaca acacctccag cagaagtaaa accaatgaca aaagaaaata ttatcaccac
    31441 ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa aggtgtacag
    31501 catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa taaatacaca
    31561 aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt atgacatgcc
    31621 tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg ttgccattga
    31681 gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat gttccgtaga
    31741 gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga acgtacaacg
    31801 gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg accaaccgaa
    31861 ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt gtccgctgga
    31921 aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag cttccatcaa
    31981 actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa agccaaaaac
    32041 ataagcgata atgatgttat tggtctgcag gatgccaaaa aagaaggcca tatagcagca
    32101 catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag
    32161 caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac ccgcggcatt
    32221 agactgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga cgcccatacc
    32281 atgcaccagg taattaaaat acaacccaag tgcggtaggg tagtagggat ttttcatagg
    32341 ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat ccgttcatta
    32401 ttgttgtaac aacgacaatt cctttttgtg gagaaattaa caaagccgct ccagatcgtg
    32461 tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat tgccatcagg
    32521 gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg
    32581 gttatttatt ttctctatga tcgcatgaag atgttttttt ctttgctgac gcatagccaa
    32641 cagttgctcg actacacaat caggaatggc acacctgcca ttttcccatt gacgccagct
    32701 ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca tggcaaaaat
    32761 atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg aaataaacaa
    32821 taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca gtatcttgtg
    32881 caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc
    32941 ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact caggggattt
    33001 ccatgcttat a
//
LOCUS       DABSYT020000045        32598 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.045, whole genome shotgun sequence.
ACCESSION   DABSYT020000045 DABSYT020000000
VERSION     DABSYT020000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.045"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..86)
                     /locus_tag="ID725_RS12690"
                     /old_locus_tag="pgaptmp_002538"
     CDS             complement(<1..86)
                     /locus_tag="ID725_RS12690"
                     /old_locus_tag="pgaptmp_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851117.1"
                     /translation="MGNIHGISCTANNYLKQAWNDIKNEYEK"
     gene            346..1266
                     /gene="cbpA"
                     /locus_tag="ID725_RS12695"
                     /old_locus_tag="pgaptmp_002539"
     CDS             346..1266
                     /gene="cbpA"
                     /locus_tag="ID725_RS12695"
                     /old_locus_tag="pgaptmp_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGV7851118.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1266..1571
                     /gene="cbpM"
                     /locus_tag="ID725_RS12700"
                     /old_locus_tag="pgaptmp_002540"
     CDS             1266..1571
                     /gene="cbpM"
                     /locus_tag="ID725_RS12700"
                     /old_locus_tag="pgaptmp_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGV7851119.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1725..2324)
                     /gene="torD"
                     /locus_tag="ID725_RS12705"
                     /old_locus_tag="pgaptmp_002541"
     CDS             complement(1725..2324)
                     /gene="torD"
                     /locus_tag="ID725_RS12705"
                     /old_locus_tag="pgaptmp_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGV7851120.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2321..4867)
                     /gene="torA"
                     /locus_tag="ID725_RS12710"
                     /old_locus_tag="pgaptmp_002542"
     CDS             complement(2321..4867)
                     /gene="torA"
                     /locus_tag="ID725_RS12710"
                     /old_locus_tag="pgaptmp_002542"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGV7851121.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(4867..6039)
                     /gene="torC"
                     /locus_tag="ID725_RS12715"
                     /old_locus_tag="pgaptmp_002543"
     CDS             complement(4867..6039)
                     /gene="torC"
                     /locus_tag="ID725_RS12715"
                     /old_locus_tag="pgaptmp_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGV7851122.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6169..6861
                     /gene="torR"
                     /locus_tag="ID725_RS12720"
                     /old_locus_tag="pgaptmp_002544"
     CDS             6169..6861
                     /gene="torR"
                     /locus_tag="ID725_RS12720"
                     /old_locus_tag="pgaptmp_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGV7851123.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6834..7862)
                     /gene="torT"
                     /locus_tag="ID725_RS12725"
                     /old_locus_tag="pgaptmp_002545"
     CDS             complement(6834..7862)
                     /gene="torT"
                     /locus_tag="ID725_RS12725"
                     /old_locus_tag="pgaptmp_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGV7851124.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            7945..10689
                     /gene="torS"
                     /locus_tag="ID725_RS12730"
                     /old_locus_tag="pgaptmp_002546"
     CDS             7945..10689
                     /gene="torS"
                     /locus_tag="ID725_RS12730"
                     /old_locus_tag="pgaptmp_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGV7851125.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            10761..11834
                     /gene="yccM"
                     /locus_tag="ID725_RS12735"
                     /old_locus_tag="pgaptmp_002547"
     CDS             10761..11834
                     /gene="yccM"
                     /locus_tag="ID725_RS12735"
                     /old_locus_tag="pgaptmp_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGV7851126.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(11883..12017)
                     /locus_tag="ID725_RS12740"
                     /old_locus_tag="pgaptmp_002548"
     CDS             complement(11883..12017)
                     /locus_tag="ID725_RS12740"
                     /old_locus_tag="pgaptmp_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGV7851127.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(12045..12275)
                     /gene="ymcE"
                     /locus_tag="ID725_RS12745"
                     /old_locus_tag="pgaptmp_002549"
     CDS             complement(12045..12275)
                     /gene="ymcE"
                     /locus_tag="ID725_RS12745"
                     /old_locus_tag="pgaptmp_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGV7851128.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12250..12438)
                     /gene="ymcF"
                     /locus_tag="ID725_RS12750"
                     /old_locus_tag="pgaptmp_002550"
     CDS             complement(12250..12438)
                     /gene="ymcF"
                     /locus_tag="ID725_RS12750"
                     /old_locus_tag="pgaptmp_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGV7851129.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12449..12661)
                     /gene="cspG"
                     /locus_tag="ID725_RS12755"
                     /old_locus_tag="pgaptmp_002551"
     CDS             complement(12449..12661)
                     /gene="cspG"
                     /locus_tag="ID725_RS12755"
                     /old_locus_tag="pgaptmp_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGV7851130.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            12947..13159
                     /gene="cspH"
                     /locus_tag="ID725_RS12760"
                     /old_locus_tag="pgaptmp_002552"
     CDS             12947..13159
                     /gene="cspH"
                     /locus_tag="ID725_RS12760"
                     /old_locus_tag="pgaptmp_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGV7851131.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            13601..13906
                     /locus_tag="ID725_RS12765"
                     /old_locus_tag="pgaptmp_002553"
     CDS             13601..13906
                     /locus_tag="ID725_RS12765"
                     /old_locus_tag="pgaptmp_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851132.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            14013..14657
                     /gene="gfcB"
                     /locus_tag="ID725_RS12770"
                     /old_locus_tag="pgaptmp_002554"
     CDS             14013..14657
                     /gene="gfcB"
                     /locus_tag="ID725_RS12770"
                     /old_locus_tag="pgaptmp_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGV7851133.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            14654..15400
                     /gene="gfcC"
                     /locus_tag="ID725_RS12775"
                     /old_locus_tag="pgaptmp_002555"
     CDS             14654..15400
                     /gene="gfcC"
                     /locus_tag="ID725_RS12775"
                     /old_locus_tag="pgaptmp_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGV7851134.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            15400..17496
                     /gene="gfcD"
                     /locus_tag="ID725_RS12780"
                     /old_locus_tag="pgaptmp_002556"
     CDS             15400..17496
                     /gene="gfcD"
                     /locus_tag="ID725_RS12780"
                     /old_locus_tag="pgaptmp_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGV7851135.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            17542..18681
                     /gene="gfcE"
                     /locus_tag="ID725_RS12785"
                     /old_locus_tag="pgaptmp_002557"
     CDS             17542..18681
                     /gene="gfcE"
                     /locus_tag="ID725_RS12785"
                     /old_locus_tag="pgaptmp_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGV7851136.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            18669..19115
                     /gene="etp"
                     /locus_tag="ID725_RS12790"
                     /old_locus_tag="pgaptmp_002558"
                     /gene_synonym="yccY"
     CDS             18669..19115
                     /gene="etp"
                     /locus_tag="ID725_RS12790"
                     /old_locus_tag="pgaptmp_002558"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGV7851137.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            19135..21315
                     /gene="etk"
                     /locus_tag="ID725_RS12795"
                     /old_locus_tag="pgaptmp_002559"
     CDS             19135..21315
                     /gene="etk"
                     /locus_tag="ID725_RS12795"
                     /old_locus_tag="pgaptmp_002559"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGV7851138.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(21435..22739)
                     /gene="appA"
                     /locus_tag="ID725_RS12800"
                     /old_locus_tag="pgaptmp_002560"
     CDS             complement(21435..22739)
                     /gene="appA"
                     /locus_tag="ID725_RS12800"
                     /old_locus_tag="pgaptmp_002560"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGV7851139.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(22819..22911)
                     /gene="cbdX"
                     /locus_tag="ID725_RS12805"
                     /old_locus_tag="pgaptmp_002561"
     CDS             complement(22819..22911)
                     /gene="cbdX"
                     /locus_tag="ID725_RS12805"
                     /old_locus_tag="pgaptmp_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGV7851140.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(22924..24060)
                     /gene="appB"
                     /locus_tag="ID725_RS12810"
                     /old_locus_tag="pgaptmp_002562"
     CDS             complement(22924..24060)
                     /gene="appB"
                     /locus_tag="ID725_RS12810"
                     /old_locus_tag="pgaptmp_002562"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGV7851141.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(24072..25616)
                     /gene="appC"
                     /locus_tag="ID725_RS12815"
                     /old_locus_tag="pgaptmp_002563"
     CDS             complement(24072..25616)
                     /gene="appC"
                     /locus_tag="ID725_RS12815"
                     /old_locus_tag="pgaptmp_002563"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGV7851142.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(25750..26607)
                     /gene="hyaF"
                     /locus_tag="ID725_RS12820"
                     /old_locus_tag="pgaptmp_002564"
     CDS             complement(25750..26607)
                     /gene="hyaF"
                     /locus_tag="ID725_RS12820"
                     /old_locus_tag="pgaptmp_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGV7851143.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(26604..27002)
                     /gene="hyaE"
                     /locus_tag="ID725_RS12825"
                     /old_locus_tag="pgaptmp_002565"
     CDS             complement(26604..27002)
                     /gene="hyaE"
                     /locus_tag="ID725_RS12825"
                     /old_locus_tag="pgaptmp_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGV7851144.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(26999..27586)
                     /gene="hyaD"
                     /locus_tag="ID725_RS12830"
                     /old_locus_tag="pgaptmp_002566"
     CDS             complement(26999..27586)
                     /gene="hyaD"
                     /locus_tag="ID725_RS12830"
                     /old_locus_tag="pgaptmp_002566"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGV7851145.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(27583..28290)
                     /gene="hyaC"
                     /locus_tag="ID725_RS12835"
                     /old_locus_tag="pgaptmp_002567"
     CDS             complement(27583..28290)
                     /gene="hyaC"
                     /locus_tag="ID725_RS12835"
                     /old_locus_tag="pgaptmp_002567"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGV7851146.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(28309..30102)
                     /gene="hyaB"
                     /locus_tag="ID725_RS12840"
                     /old_locus_tag="pgaptmp_002568"
     CDS             complement(28309..30102)
                     /gene="hyaB"
                     /locus_tag="ID725_RS12840"
                     /old_locus_tag="pgaptmp_002568"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGV7851147.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(30099..31217)
                     /gene="hyaA"
                     /locus_tag="ID725_RS12845"
                     /old_locus_tag="pgaptmp_002569"
     CDS             complement(30099..31217)
                     /gene="hyaA"
                     /locus_tag="ID725_RS12845"
                     /old_locus_tag="pgaptmp_002569"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGV7851148.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            31643..31730
                     /locus_tag="ID725_RS12850"
                     /old_locus_tag="pgaptmp_002570"
     tRNA            31643..31730
                     /locus_tag="ID725_RS12850"
                     /old_locus_tag="pgaptmp_002570"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31677..31679,aa:Ser,seq:tga)
     gene            32331..32598
                     /gene="tccP2"
                     /locus_tag="ID725_RS12855"
                     /old_locus_tag="pgaptmp_002571"
                     /pseudo
     CDS             32331..32598
                     /gene="tccP2"
                     /locus_tag="ID725_RS12855"
                     /old_locus_tag="pgaptmp_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT         7989 a         8588 c         8276 g         7745 t
ORIGIN      
        1 tttttttcgt actcattttt tatatcgttc caggcttgct tcaaataatt atttgcagtg
       61 caactaattc catgtatatt acccatatat agcgcctata aaatttaata aataataacg
      121 cactagtaaa acttaaagtg cctgtttcaa ctatcaaaaa tcgctcaccc tttttcacct
      181 gtttaaaata tgttcagcgc cccaccttga ggcgacctcc tctccgcgat gatttcaata
      241 acatattctg tgttggcata tgaaattttg aggattaccc tacacttata ggagttacct
      301 tacaggggtt ccttcaattt gtgttgattt acgcgagata acgctatgga attaaaggat
      361 tattacgcca tcatgggcgt gaaaccgacg gacgatctca agacaatcaa gaccgcctat
      421 cgtcgacttg cccgcaaata ccatcctgat gtcagcaaag aaccggatgc cgaagcccgc
      481 ttcaaagagg tcgctgaagc ctgggaagtg ttaagtgatg aacaacgtcg cgctgagtat
      541 gatcagatgt ggcaacatcg caacgatccg caatttagcc gtcagttcca gcatggcgac
      601 ggtcagagtt ttaacgccga agattttgac gatatcttct cgtcaatttt cggtcagcat
      661 gcccgccaga gccgtcaacg ccccgccgca cgcggccacg atattgaaat cgaagtggcg
      721 gtattcctcg aagaaacgct tactgagcat aagcgtacca tcagctataa cctgccggtt
      781 tataacgcct ttggcatgat cgaacaggaa atcccgaaaa cgctgaatgt gaagatcccg
      841 gcaggcgtcg gcaatggtca acgcatccgc ctgaaaggcc aggggacgcc gggcgaaaac
      901 ggcggtccaa atggcgattt gtggctggtg attcatattg cgccacatcc gctgtttgat
      961 attgtcggcc aggatctgga aattgtggtg ccggttagcc cgtgggaagc ggcgctgggt
     1021 gctaaagtca ccgttccaac actgaaagaa agcattttgc tgactatccc gcctggcagc
     1081 caggccgggc aacgattgcg cgttaaaggc aaaggtctgg tgagcaaaaa acagaccggc
     1141 gatctgtatg cggtactgaa aatcgtgatg ccgccgaaac cggatgaaaa cactgccgcg
     1201 ctgtggcagc aactggcaga cgcccagtcg tcttttgatc cacgtaaaga ttgggggaaa
     1261 gcataatggc taatgttacg gtgactttta ctattaccga attttgcctg cataccggca
     1321 tctctgaaga ggaattgaat gaaattgtcg gtttgggggt ggttgaaccg cgtgagatcc
     1381 aggaaacaac ctgggtattt gacgaccatg ccgccatcgt ggtgcaacgc gcggtgcgcc
     1441 tgcgtcatga actggctctg gactggccgg ggatcgcggt ggcgctgacg ttaatggatg
     1501 atattgcgca cctgaagcag gaaaaccgcc tgctgcgcca gcggctttcc cggtttgtag
     1561 ctcatccgtg atgtgtgttt gccggatgtg atgacgcatc ttagcctgcc tgcaaagagt
     1621 acttttcctg tgggccggat aaggcgtgtt cacgccgcat ccggcggcgg tgccctaatg
     1681 cctgatgcga cgctaaacgc gtcttatcag gctaacaaac gacgttatct gttttggtgg
     1741 tcgccctcaa ctaacaccag caataactgg cttagtgccg cgtaaaaacc aaagctgtca
     1801 tactgatggc aacgcgcaac aaactctggt aaccattgcc gcaacgccgt gagtgttttt
     1861 tgccgtaaac tgtcgattct tcgcgcagga accgtcccct ctcccagcga aaaatgcaga
     1921 tgactcaaca actcgagata gatcgccaga tgatctgtcg gttcgttgaa attgccgctg
     1981 gtttccatcc ctgcctcaac taacaagcgt ttaatctctt gctcgtcctg tttgtaggcc
     2041 gatgcatacg gcagcgccgc ttgtttgtca gtcatcagaa acaggccgca aaagtcagcg
     2101 gccagttcca gacgggcatc gtcacgtact gtcagagtgg caacacggtt ttccagctca
     2161 tccaccgccg cagtgagcgg cggttcgctt ttcagcaacg aaaaccattc agccatctgc
     2221 gcactggcga tttgcgtcag ttgttcatcg tccagttcac gggagaacaa ctgcgccagc
     2281 caggcgtaaa cacaggctat ctgttgtgct gtcagcgtgg tcataatttc acctgcgacg
     2341 cgggaacata ttcgcactgc gccaccatct ccacggggcc gttaaacgcc gttacctgct
     2401 ccactgttcc gttgcacttc tcaatttcca ccagcgtagt gtgcgcactg gtcgcctgcg
     2461 ccagctgcga agtaccgatg tcaatggtca acacgttggg gttaccgtat ttgcacagcg
     2521 caccaggctc gccgccttta tctggatcgt gccatgcccc ttcgtgaatt cgcgccacgc
     2581 cgggtgcata gcggtcagaa actactgccc ctgccaacac ctgaccgcga gcgttaaaga
     2641 cgcgtaccac atcaccgtta cgaataccgc gcgcgctggc atcctgcggg ttaatgaaca
     2701 ctggctcttt acccgctacc gtatattgct gacgcagagt ttccgactca cataactgcg
     2761 agtgaagtcg gaaatccgga tgcacagatt gcagatgcaa cggatacgtc tgcgagccgg
     2821 gcccaccgtg ggagcgttcg attttctcaa accacatcgg atgcccctga caatcgtcgt
     2881 agttcatatc ggcgatggtt ttcgaataga tctcaatcag gccactcggc gtgcccagcg
     2941 gttcgagatc tggatcttcg cggaatgcct ggtggcgaac aaacatctgc ggatggtcaa
     3001 actcgacgta ctctttgtta ttccagaagt catcaaacgc tggcaaatga acgccgcgtc
     3061 ctttgccttg ctgaacacct tcctgccaga tgcgtttcag ccagcccatt tcgtccagcc
     3121 cttcggtaaa ggcttcttcg cgattaaagc ggcggcacag ctcgcggaaa atatcaaagt
     3181 cgttgcgcgc ctcgaactgc ggcggcacga cctgtttcat ggcgataatg ccacggttgg
     3241 agtggttgcc gtactggtcg agatcgttac gctcaaactg cgtggtcgca ggcagcacga
     3301 tatcggcaaa gcggcaggtt gaggtccact ggttatctat ggcgataacc gtttccagtt
     3361 tgcgccagcc ttcaataatg cggttgatct gctgatggcg atggaagggg ttagttccgg
     3421 caaaaataca cattttcagc ggcggcagtt ttaccgattt accgttccag ttgattactt
     3481 tccccggttc gaggatcgca tcgataaaac gggcaatcgg aatggtgctg ctgtagcctt
     3541 tgtaatcact gttgtcgtga acaggcggaa tcgacgtaga gccggagaaa ccactcagaa
     3601 taacgccttt acgccccggc gtgcctgcgc cgttatagtg ccagccaaaa ccaaagccac
     3661 cacctggcag gccaatttgc cccagcatcg ccgccagaac cacaatcatc cacgcccact
     3721 gttcaccgtg ctgcatacgc tgtacgcacc agccagcaat aatctgcgtt ctgttcgccg
     3781 ccatctgccg cgccagtcca cgaatggttt cggcatcaat gccggtcagt ttttcagccc
     3841 atgcggcatc tttcggctga ccgtctttct cacccagcag atacggcagg aactgctcaa
     3901 aacccacaca gtagttagcg aggaagtttt tgtcgtacag gttttcactg tacaacgtat
     3961 gcgccagcgc cagttgcagc ggcacgtcag tttgcgggtt aaccgcaatg tgcttcacat
     4021 gctcgcgccc cagatactca tgggtggatg taacaaccgg atcgatgcta atgacctcaa
     4081 tttcaccggc ggcgactttc gctttcaact gcgcgtaata ttcataaaca tcgtgatccg
     4141 ggcaccacca gttcgcttgc tggtttttca gcaaatcgga gccccacagc acaatggttt
     4201 tgctgttctg caataccagc ggccaggagg tttgctgttc atacacttcc atcgaaccga
     4261 ctacgcgcgg caggatcacc tgcgcagcac cagtagagta atctccgccc gtaccaacgc
     4321 tattaccatg caaggcaata gctttcgcca gcatccccga agcgttatgg aacatccccg
     4381 tcgattgcca accactggcg gtcagcaagg cactcggccc gtgagttttc tgtacgcgtt
     4441 ccagttcttc atagaacatg tcgagggctt catcccagct cacacgcaca aaacggttat
     4501 caccgcgctg ggaggtgtcg ctcagatggc gcttacgcag ccagtccacg cgtaccatcg
     4561 gataacgaat acgcgccgcg ttgtgtacat gatccggcaa tccggcaatc attttcgacg
     4621 gatatttatc cagttcgaac ggttttgccg ccacaaagcg accatccttc accgtcgcgc
     4681 ggatagcccc ccagtgcgac ccggtaagaa tgccctcttt cgagatgaca gcctcagtcg
     4741 ccgcttgcgc cgcagtcgca cggcgcgacg ttaacaatga cggccccagc atcccggcga
     4801 cggttaagcc gccgagttgt gccagaaaac gccgacgtga tgcctgaaag agatcgttat
     4861 tgttcattat ttttcttcct tcttatcgcc gtgagcctta cctgcggtgt cagacgcatt
     4921 catttgcaga tatttcaaca aggtgcgttc ttcacgttta tcgaggctgg taaagccgat
     4981 catgccgttg agcgtgccaa tccagccgtt agcgtcaaag tgagagattt ccggtgcgcc
     5041 gtggcactgg ttacaggtgc cgttgtacaa cgaatccgca taagcccaga tcggtttgat
     5101 atcgttcacc atgtcacctt tcttcatcca cgcggtggcc tgcaacttgc tccactcggt
     5161 attggtgtcg gcaacggtgg ttttctccag cgtttttacc tgctgctgca catcaccacg
     5221 aatcgaggca acaaagatgc gtttacctgg gaattgggtg agtacacgct gacgtccggc
     5281 gctttctgtc cagccggtaa tttcaatttg cagccagtcg ccgtcacgtt taaggacttt
     5341 cacttccgag gcaggcagca gagaaccaga ggcttcttta tcgcctttcg ccgcataaat
     5401 tggcttaata tcaatagagt acagcgtgtc accactgtca ttagcaccgg cgcgcagctc
     5461 atcgaactgc ttacggaagc cgctactcat atccggtaac tggtgggcaa tacctttatg
     5521 acagtcgatg caggattgat tatctttcgc tgccaccttc atctgacgtg ccgcttcagg
     5581 atgctgcttc gcatgatcca tcgcatcgta gttatggcag gagcgacagg ttgccgagtt
     5641 gttttctttc attcgcgccc attcacgctc ggcaagttcc gcgcgtttgg cttcaaattt
     5701 ttcaggtgta tcaatggagt gggcaataaa ggtctggtag atatcgttgc tcgcttccag
     5761 tttgcgcttc accatgcctg gaatatccgg cgggatatga cagtcatggc attcagctcg
     5821 cacgccggag gcgttctgga aatgcaccga ctgtttatat tcttcataca ctggttgcat
     5881 actgtggcaa ctgacacaaa attcggttgt gctggtgact ttgatcccaa cgtgtggcaa
     5941 tacaatcagc gcaatgccaa tcacaatccc aatagcgacc agcgccagta ccgaccaacg
     6001 agcactgggt cggcgtagcg cgttccagag tttccgcata atagcccctg taaaattatg
     6061 gtttagtgaa gcgatcttaa tgagtaaata tgaacagcgg cactggtcag gatgaacggc
     6121 ttacggcaga atatgaacag atatgaacag aatgagtaaa accctctgat gccacatcac
     6181 attgttattg ttgaagatga gccggttacc caggcgcgat tacaatccta cttcactcag
     6241 gaggggtata ccgtttccgt tacggcgagc ggtgccgggc tacgggaaat tatgcagaat
     6301 cagccggtgg atttaattct gctggatatc aacttgcccg atgaaaatgg cctgatgtta
     6361 acccgcgccc tgcgagaacg ctcaacggtg gggattattc tggttaccgg acgcagcgat
     6421 cggattgacc gtattgttgg gctggaaatg ggcgcagacg attacgtcac caaaccgctg
     6481 gaactgcgcg aactggtagt acgggtaaaa aatctgctct ggcgaatcga cctcgcgcga
     6541 caagctcaac cgtacactca ggacaactgc tatcgctttg cgggttattg cctgaatgtg
     6601 tcgcgccata cgctggagcg ggatggtgag ccgattaaac tgacccgcgc agagtatgaa
     6661 atgttggtgg catttgtgac caatccgggc gaaattctca gccgtgaacg tctgctgcgt
     6721 atgctctctg cgcgtcgggt ggaaaaccct gacctgcgca ccgtcgatgt gttaattcgt
     6781 cgtttacgcc ataaactcag cgcggattta ctggtgacgc aacatggcga aggttatttc
     6841 ttagccgctg atgtgtgctg ataaaaatag accggacgaa atcccccagg cgacagcgag
     6901 cggcggatat gttcgcggtc ggcatttttc ggcgtcagaa ctaaaatcgg tgggctgaca
     6961 ttatcagaca ccgattgccc ctgtaattgc ctgatggcct gctcaactgc cagttccccc
     7021 tgccagacca tttgatcgct ggcagccata atcactcttc cccgcttcag cccgcgatac
     7081 acctgatgtg aaagataaaa cgacaccacg gtaagcggcg ttttcaggtt acgcccttcc
     7141 cccattgccg cctctgccgc aatggccgtt ccggcaacga cgtcgatttc tggatggcgt
     7201 tccagcatct cctgcaacag gttacgctgg atttcaatat cgttatcacc aagcgcaata
     7261 tcaacaatac gcaccgggct tccggcaatg gctgcgcgaa aaccctcgac catctcctta
     7321 ctgcccccgg cgttatcggg tccgggcatc aacagcacat tcagtggttt accgtgcgcc
     7381 cattgcacca gatatcgccc aggttgatag cccatctgaa accagggcac accaacgcgg
     7441 cttttcacct ggggagcatc aatagcattt accagctcga tcaccggcag atttgctact
     7501 tgcttttgca gatcgggaaa tgacgtcgtg ctactaccga gcaaaatggc ctctgcgccc
     7561 cactgtttac actggtcgat ttgtgcttgc tgggtagcca actggctgta gccgcctgcc
     7621 tccagcactt ttaaatccac accgtagcgg cgagcagcct cctgcatacc atagttcaac
     7681 gataaccaat atgaatcttt caggctggga taaagcgcgc acagtttcca tacgcgtttg
     7741 gcttttagcg gcatagaggc ttgcaccgtg aaatgctgcg catcatgcca gcgcaacagg
     7801 ttatcagccg aaaatgccgg caacatgaaa agggaaagaa gtaaaaatag cagcacgcgc
     7861 atgatagcct catcaataat aaggctttat gctagatgca ttccgctttg cgactcaaca
     7921 tttttcacct taagtgcacc gaccgtgaat ttaaccctga cccgaagact ctggatgggc
     7981 tttgccctga tggcgctgtt aaccctgacc agtaccctgg tgggatggta caacctgcgc
     8041 tttatcagcc aggtggaaaa agacaatact caggcattga ttcctaccat gaatatggcg
     8101 cgccagttga gcgaagccag cgcctgggaa cttttcgccg cgcagaacct gaccagtgcc
     8161 gataacgaaa agatgtggca ggcgcagggg cgaatgctca ccgcacaaag cctgaagatt
     8221 aatgcgttgc tgcaagcgtt acgggaacaa ggttttgata ccaccgctat tgaacaacag
     8281 gagcaggaga tctcccgttc gttacgtcag caaggggaac tggtggggcg gcgtctgcaa
     8341 ctgcgccagc aacaacagcg actcagtcag cagatagtcg ctgccgccga tgagatcgca
     8401 cgcctggcgc aaggtcaggc gaataatgcg gcaacctccg ccggagcgac ccaggccggg
     8461 atttacgatt tgatcgaaca agatcagcgt caggctgctg aaagtgcact cgatcggctg
     8521 attgatatcg atcttgagta tgttaaccag atgaatgaac tgcgccttag cgctctgcgg
     8581 gtgcagcaaa tggtgatgaa tctggggctg gagcagatcc agaaaaatgc gccaacgctg
     8641 gaaaagcagc tcaataatgc ggtgaaaatt ctgcaacgtc ggcaaatacg cattgaagat
     8701 ccgggagttc gtgctcaggt cgcaacaacg ttaactaccg ttagccaata tagcgatttg
     8761 ctggcgctgt tccagcagga cagtgaaatc agcaatcacc tacaaactct cgcacaaaat
     8821 aacatcgccc agttcgcgca gtttagcagc gaagtcagtc agctggtcga caccattgag
     8881 ctgcgtaatc agcacggact ggcgcatctg gaaaaagcca gtgcacgcgg gcaatacagc
     8941 ctgttattgc tggggatggt ttcactttgc gcactgattc tgatcctctg gcgcgtggtt
     9001 tatcgctcag tcacgcgtcc acttgccgaa caaacgcagg cgctgcaacg gctgctggac
     9061 ggtgatattg actccccttt cccggaaacc gctggcgtac gggagctgga caccatcggg
     9121 cggctgatgg atgcgtttcg cagcagtgtt catgcactga atcgccaccg cgaacagctg
     9181 gcggcgcagg tcaaagcgcg tacagctgaa ttgcaggaac tggtgataga acaccgacaa
     9241 gcacgggcgg aagcagaaaa agccagccag gcaaaatcgg cgtttctggc ggcgatgagc
     9301 catgagatcc gcacaccgct gtacggtatt ctcggcaccg cacagctact ggcagataac
     9361 cccgcactta acgcccagcg tgatgatttg cgggcaatta ccgactccgg cgaatcgttg
     9421 ctgaccatcc tcaacgatat tctcgattat tccgctatcg aagcaggtgg caagaatgtt
     9481 tcggtcagcg atgaaccctt tgaaccgcga ccgctgctgg aaagtaccct gcaattaatg
     9541 agtggacggg tgaaaggtcg cccgattcgc ctggcaacgg caattgccga cgatgtaccg
     9601 actgcgttaa tgggcgatcc gcggcgtatt cgtcaggtca ttaccaacct gttgagcaac
     9661 gccctgcgtt ttactgacga agggcagatc gttttacgta gccgtactga tggtgagcaa
     9721 tggctggtcg aagtggaaga cagcggctgc ggtattgatc ccgcgaaact ggcagaaatc
     9781 ttccagccat ttattcaggt aagtggcaaa cgcggcggca ccgggctggg gctgactatc
     9841 agtagccgtc tggcccaggc gatgggcggc gaactgagcg ccaccagcac gccggaggtt
     9901 ggaagctgtt tttgtttacg cttgccgtta cgtgttgcca cggcacccgt gccaaaaaca
     9961 gtcaatcagg cggtgcgtct tgacggttta cgtttgctgt taattgaaga taacccgcta
    10021 acccagcgaa ttaccgttga gatgctgaac accagtggtg cgcaggttgt tgctattggc
    10081 aatgccgcgc aggctttaga gacactgcaa aatagcgaac cgtttgctgc cgcactggtg
    10141 gattttgatc tgccggatgt caacggcatt acgcttgccc gacaactggc acgacaatat
    10201 ccgtcgctgg ttttgattgg ctttagcgcc catgtcattg acgaaacgct gcgccagcgt
    10261 accagttcac tatttcgcgg gattatccct aaaccggtgc cgcgtgaagt gctcggtcag
    10321 ttactggcgc actatctcca actgcaagcc aataacgatc taccactgga tgtatcgcaa
    10381 ctcaatgaag acgctcagtt aatggggacg gagaagatcc acgaatggct ggcattattt
    10441 acacaacatg ccctgccgct tctcgatgaa atcgacattg cccgcgccac tcaggatagc
    10501 gagaaaataa agcgtgccgc gcatcagcta aaaagcagtt gctcgagtct gggaatgcgt
    10561 agcgccagcc agctctgcgc acaactggag cagcagccat tatctgcccc ccttccacac
    10621 gaagaaatta cacgcagtgt tgccgctctg gaagcatggt tgaataagaa agacctgaac
    10681 gcgatttgaa tcaatatcag ccagttaaga ataactagaa tagtggcatc tggtatgcaa
    10741 cttaacggac tggcaaaatc atggcagaga aaaaaagaac ccgctggcag cggcggccag
    10801 gcacgacggg cggcaaatta ccgtggaatg actggcacaa tgccacgacc tggcgtaaag
    10861 cgacgcaatt attgctgttg gcaatgaata tttatattgc catcacgttc tggtattggg
    10921 tgcgctatta cgaaacggca ggtagtacga tatttgtcgc cagaccggga ggtatcgaag
    10981 gctggctacc gattgccggt ctgatgaatc tgaaatatag ccttgcaaca ggtcagttac
    11041 cgtccgtcca cgccgccgcg atgctgttat tggtcgcttt tatcgtcatc agtctattac
    11101 tcaaaaaggc cttttgctca tggttatgcc cggttggtac gctttctgaa ttaatcggcg
    11161 ctctcggtaa caaactgttt ggtcggcaat gtgtccttcc ccgctggctg gatattcctc
    11221 tgcgcggtgt aaagtatttg ctgttgagtt tttttctcta tatcgcgtta ttgatgcctg
    11281 ctcaggcgat tcactatttt atgttgtcgc cctacagcgt ggtgatggac gttaaaatgc
    11341 tcgatttctt tcgtcatatg gggaccgcga cattaatcag cgtgaccgtt ttgctgattg
    11401 ccagcctgtt tattcgccat gcctggtgtc gttatctttg cccatatggc gcgctgatgg
    11461 gcgtggtttc gctattatcg ccgtttaaga ttcgtcgcaa tgccgaaagt tgtatcgatt
    11521 gtggcaaatg cgcaaaaaat tgcccatcac ggatcccggt cgataaatta attcaggtac
    11581 gaacagtgga atgtaccggc tgtatgactt gcgtagagtc atgtccggta gcctcaacat
    11641 tgaccttttc actgcaaaaa cctgcggcaa ataaaaaagc ctttgcgttg tctggctggt
    11701 taatgacgct actggttctg gggattatgt ttacggtgat tggttacgca atgtatgcag
    11761 gagtatggca aagcccggtg ccggaggaat tgtaccgacg cttaattccg caagcgccaa
    11821 tgattggtca ctaaattagc aacaagcgga acatcacctc gcacggggtg atgttcacaa
    11881 aatcacatta ttttgatttt gacatcataa ctttcaagcc cggtcatttt ttctcgcgcg
    11941 gtgaagcgaa tttcagagac ttctttccct gtattcttgt tcagctcggc gattttttgc
    12001 gcgataaagt cggcgatttc cgtttcggct tgttttttaa ctcttcaata ttcactgtta
    12061 acctcttctg gtcttaaaag acagcaacac gccagtaagg tgttggttcc cggtaccctg
    12121 gtcaggtaag aaatgttata agccatctcc ccgaaagatt caataatcaa caggttatcg
    12181 cttactatac atacagcgtt gaaaaaaaat aagacagata taaaaaaggc tccacaaggg
    12241 agcctgatat tattttgtga aatccagcgg tgcatggtca gtgcgtattt gcagcgcgac
    12301 gttatcaaag gtgatcatcg tcgatttaca aaaaatacac ttagcgccca aagggtttcg
    12361 ctccgtcaca tcgaatgccg atgtgcggta ctgtgaacca tgacaacatg gacagcgaaa
    12421 atggagatgt tgagtaataa cggcaacctt agagcgtaac tacgttcgct gccgcggggc
    12481 cacgttgacc ctgctcaata gaaaattcaa ctttctgatt ttcgttcagc gtgcggaatt
    12541 cattgctctg gatggcggtg aaatggacga aaacgtcttt gctgccatca tcaggagtga
    12601 taaagccaaa acctttatct gcgttaaacc attttactaa accagtcatt ttattagaca
    12661 tattttactt ccttcatttt gggcctttcg gcaaacaggg ctttaataca gaaactactt
    12721 agtgttcagg agagagactc aaaagaaggg gataatgcct gataatgaga actgctttag
    12781 taaactactt tgtatgctgt ctgtctttcc aaccgacgca gctattaacg catgataatt
    12841 gagattaagc aaattttatt ttagccgtcc ggcggtctta aatatcgctg gctttatttt
    12901 attaaccccg tatagtgcag cgcagtattt tcagtaagga atttgtttgt cccgtaaaat
    12961 gacaggaatt gtcaaaacct ttgatcgcaa aagcggcaaa ggattcatta tcccctccga
    13021 cggtcgtaaa gaagttcagg tccatatttc cgcattcact ccccgcgacg cagaagtgct
    13081 tataccagga ttacgcgtgg aattttgccg cgtaaatggc ctgcgaggac caacagcggc
    13141 aaatgtttat ctctcttaat atgacgctgc ttgtttaaag ctgactggtt ttattggcat
    13201 agttcaatag tcaacaccat gtattataac acaagttact ttttcaccgg aaaactaatc
    13261 catttaaccg aatatctcta caactaatag ttataaatgc cacttaacag cgagttatag
    13321 taccacttga aactatattg ccaacaatag ttaatagctt tcttaacaat tttcttatct
    13381 ttaatttgta ttttgctaag atttatctta tgctcacagg tcgtatttcc agaggggaat
    13441 agttaacaat cttatatttc cctctgcgcg cctttctcac ccgctgtata tagtgcaagt
    13501 ggtatgagga aagggagatt tcacaaggat gttatactca aagtagacaa tcctgatctc
    13561 aagcgtacgt attgtccatg cgatgaataa aggaaaagtt atgaaacaca agctttctgc
    13621 aatccttatg gccttcatgt tgacgacgcc agctgcgttt gctgcccctg aagcagcaaa
    13681 cgggaccgag gcaacgacgg gtactactgg caccacaacg accactaccg gtgcaaccac
    13741 gacggctgct accactggtg gcgtggctgc tggcgctgtc ggcacggcga cagttgtcgg
    13801 tgtggcaacg gctgttggtg tggcgacgtt ggcagtagtt gcagcaaatg acagtggcga
    13861 tggcggttct cataacacct caaccaccac gtccactacg cgctaattct gcttaaacgc
    13921 gtcgaaatta aagttaaata atacccggtt gcgatattcg taccgggctt tagagatttg
    13981 cattttttta attaaccttt caaggattaa aggtgcgccc tcttatttta tcgattttcg
    14041 cactatttct tgcgggatgt acgcacagcc agcaaagtat ggtcgataca tttcgcgcca
    14101 gccttttcga taatcaggat atcaccgtag cggatcagca gatccaggcg ttgccttatt
    14161 ccacgatgta tttacgcctt aatgaagggc aacgaatctt tgtggtactg ggatatatag
    14221 aacaagaaca aagcaaatgg ttatcccagg ataacgccat gctggttacc cacaatggac
    14281 gtcttttaaa gaccgtcaaa cttaataata atctgctgga agtgacaaat tccgggcagg
    14341 accctctgcg taacgcgctg gcaataaaag acggcagccg ctggacgcgc gatattctct
    14401 ggagtgaaga caaccatttt cgctctgcga ccctgagttc cactttttcc tttgctggat
    14461 tagagacgct gcatattgcg ggtcgcgatg tgctgtgtaa tgtctggcag gaagaggtga
    14521 cttccacgct accagaaaaa cagtggcaaa acacattctg ggtcgattcg gctactggcc
    14581 aggttcgtca aagtcggcaa atgttaggcg caggggttat tcccgtagaa atgacgtttc
    14641 ttaaacccgc accatgaata aattacagtc gtatttcatt gccagcgtac tttacgtaat
    14701 gacaccccat gcctttgcgc aaggaacggt gactatttat ctgcctggcg aacaacagac
    14761 actttccgtt ggccccgtgg agaatgttgc ccagttagtg acacaaccgc aactgcgcga
    14821 tcgcctctgg tggcctggtg ccctgctgac cgactccgcg gtgaaagcta aagcgcttaa
    14881 agactaccaa catgtcatgg cacagcttgc ttcctgggaa gcagaagctg acgacgatgt
    14941 ggcggcaacg attaaatccg tgcgccagca gttactcaat ctcaatatta ctggtcgctt
    15001 accggtcaaa ctggaccctg attttgtacg agtggatgag aacagcaatc ccccgctggt
    15061 cgggaactat acgctctata ccgtacagcg cccggtgact attactcttc tgggtgcggt
    15121 ttctggggct gggcaattgc cctggcaggc aggccgtagc gttaccgatt atctgcaaga
    15181 tcacccccgt cttgcaggcg cggacaagaa taacgttatc gtgattacgc ctgagggtga
    15241 gactgtcgtc gcccctgttg ccctgtggaa taaaagacat gtggaaccac cgcctggcag
    15301 ccagctttgg ttaggctttt ctgcgcatgt actgcccgaa aaatatgctg atttaaatga
    15361 ccagattgtg tctgttttaa cgcaacgtgt tcctgactga tgaagaagaa ttcttatctt
    15421 ttaagctgcc tggccattgc cgtctccagt gcctgtcatg ctgaagtatt aacctacccg
    15481 gatccgctgg gttcgtcgca atcggacttt ggcggcacag gattgttgca gatgccaaat
    15541 gcgcgcatcg caccggaagg tgaattcagc gtcaactacc gggataacga tcaataccgt
    15601 ttctactcca cctccgtggc gctgtttcca tggctggaag gcaccattcg ttatacggat
    15661 gtgcgcacac gtaaatatag ccagtgggaa gatttcagcg gcgatcagtc atacaaagac
    15721 aaatcattcg attttaaact tcgcctgtgg gaagaaggtt actggctacc gcaagtggcg
    15781 tttggtaaac gtgatattgc tggtacgggt ctgtttgacg gtgagtatct ggtggccagc
    15841 aagcaagcgg ggccatttga tttcaccctc gggatggcat ggggctacgc cggtaatgcg
    15901 ggcaatatta ccaacccgtt ttgccgggtg agcgataaat attgtcatcg cgcagagtct
    15961 cacgatgcgg gcgatatcag ctttagcgat atctttcgtg gcccggcttc catctttggc
    16021 ggcattgagt atcaaacgcc gtggaatccc ctgcgtctga aactcgaata cgacggcaac
    16081 aattaccaga atgatttcgc tggcaaactg cctcaggcaa gccatttcaa cgtcggtgca
    16141 gtttatcgcg ctgccagctg ggcagacctc aacctgagtt atgaacgcgg taacacgttg
    16201 atgtttggtt tcactttacg gaccaatttc aacgatctgc gccctgccct gcgcgatacg
    16261 ccaaaaccgg catatcaacc tgcgcctgaa tctgaaggat tgcagtacac cacggtagca
    16321 aaccaactta ccgccctgaa gtataacgcg ggctttgacg cgccagaaat tcagctacgc
    16381 gataagacgc tgtatatgtc tggtcagcaa tacaaatacc gtgactctcg tgaagcggtc
    16441 gatcgtgcca accggattct ggtgaataac ctgccgcaag gcgttgagaa gattagcgtg
    16501 acgcaaaagc gcgagcatat ggcgatggtg actaccgaaa ccgacgtagc cagcctgcgc
    16561 aaacagctgg caggtacagc gcctggtcaa tcagagccac tgcaacaaca acgtgttgaa
    16621 gctgaagatc tttctgcctt tggtcggggc taccgtattc gtgaagatcg ctttagctac
    16681 tctttcaacc caacactttc acagtcgctg ggcggcccgg aagatttcta tatgttccag
    16741 ctggggctga tgtccagcgc ccgctactgg tttaccgacc acctgctgct tgatggcggt
    16801 attttcacca atatttacaa caactacgac aagtttaagt cttcgctgtt gcccgcggac
    16861 tctaccctgc cccgcgtgcg cacgcatatc cgtgattacg ttcgcaatga cgtttatctc
    16921 aacaacttgc aagcgaatta ctttgccgac ttaggcaatg gtttctatgg ccaggtgtat
    16981 ggcggttatc tggaaacgat gtatgccggt gtcggttccg agctgcttta tcgcccgcta
    17041 gatgccagct gggcgctggg tgtggacgtt aactacgtga agcagcgtga ctgggacaac
    17101 atgatgcgct tcaccgatta ttccacgcca actggtttcg tgacggctta ctggaacccg
    17161 ccgacgctca atggcgtact gatgaaactt agcgttgggc aatatctggc aaaagataaa
    17221 ggggcaacga tcgacgtcgc caaacgcttt gacagcggcg tggcggtagg ggtatgggcg
    17281 gcaatcagta acgtatctaa agatgactac ggcgaaggcg gctttagtaa aggtttttat
    17341 atctcgattc cgttcgactt gatgaccatt ggacctaacc gcaaccgcgc ggtggtttcg
    17401 tggacaccat tgacgcgtga tggtggacaa atgctgtcac gcaaatacca gctctatcca
    17461 atgacggcag agcgagaagt accggttgga caataacctg acgtaattca taacattaca
    17521 ttgactgata aatataaacc aatgaaaaag aacattttta agttttcggt actgacattg
    17581 gctgttctgt ctttgacagc atgtaccctt gttcctggtc agaatttatc gaccagtaac
    17641 aaggatgtca ttgaactacc tgacaatcag tatgacctgg acaaaatggt caatatctat
    17701 cccgtcacgc caggattgat tgatcagttg cgcgccaaac cgattatgtc gcaggctaac
    17761 ccggagctgg agcagcaaat cgcgaactat gaatatcgta tcggcattgg tgatgtgttg
    17821 atggttacgg tctgggatca cccggaacta accacccccg cagggcaata ccgtagcgcc
    17881 agtgataccg gcaactgggt taacgccgat ggtgcgattt tctaccccta tattggtcgc
    17941 ctgaaagtgg ccgggaaaac acttacccag gtacgcaacg agattacagc gcgtctggat
    18001 tcagtcattg aaagcccgca agtcgatgtc agcgtcgcgg cgttccgctc gcaaaaagcg
    18061 tacgtcaccg gtgaagtcag caaatcgggt cagcagccga ttaccaatat tcctctgacc
    18121 atcatggatg cgattaacgc cgcgggcggt ctgaccgcag atgcagactg gcgcaatgtg
    18181 gtgctcaccc agaatggcgt aaagaccaaa gtgaacctct atgcgctgat gcagcgtggc
    18241 gacttgcgcc agaacaaact gctgcacccg ggagatattt tatttattcc gcgcaacgat
    18301 gatctgaaag tcttcgtgat gggtgaagtg ggtaaacaga gcacgctgaa aatggaccgc
    18361 agcggtatga ccctggcgga agcgctgggt aacgctgagg gtatgaatca ggatgtcgct
    18421 gatgcgacgg ggatttttgt tattcgtgca acgcaaaaca agcagaatgg caaaatcgcc
    18481 aatatttatc agctgaatgc gaaggacgca tcggcaatga tactcggaac ggaattccag
    18541 cttgaacctt acgacattgt ttatgtcact accgcgcctc tggctcgttg gaaccgtgtg
    18601 atttccctgc tggtaccgac gatttccggg gtgcatgacc tgacagagac ttcacggtgg
    18661 attcaaacat ggcccaacta aaatttaact caatcctggt ggtttgtacc ggcaacattt
    18721 gccgttcgcc cattggcgag cgcttgctgc gtaaacgtct gccgggcgta aaagtcaaat
    18781 cggcaggtgt tcatggtctg gtaaaacacc ctgcagatgc gacagcggca gatgttgccg
    18841 ctaatcatgg cgtatctctt gaaggacatg ccggacgtaa gctcactgct gagatggcac
    18901 gaaattacga tctgattctg gcaatggagt cggaacatat tgctcaggtt accgcaatcg
    18961 ctcccgaggt ccgcgggaag acaatgctgt ttgggcaatg gctggaacag aaagagatcc
    19021 cggatcccta tcgtaaaagt caggatgcat ttgaacatgt ctacggaatg ttggagcgcg
    19081 ccagtcagga atgggcgaag cgcctcagcc ggtaatttga gtttataaaa tacgatgaca
    19141 actaaaaata tgaatacgcc accaggcagc actcaggaaa atgagatcga tctgcttcgt
    19201 ctggtcggcg agttatggga tcaccgtaag tttattatca gcgtgaccgc gttattcacg
    19261 ctgatcgctg tcgcttactc gctgttaagc acaccaattt atcaggcaga tactctggtc
    19321 caggttgagc aaaaacaggg caacgccatt ctcagcggcc tgagtgatat gatccctaac
    19381 tcatcgcccg agtctgcacc ggagatccaa ctgctgcaat cgcgcatgat tctcggtaaa
    19441 accattgctg aactgaatct gcgcgacatg gttgagcaga agtattttcc gattgtgggt
    19501 cgcggctggg cgagattaac caaagagaaa ccaggtgagc tggcgatcag ctggatgcat
    19561 attccacaac tgaatggtca ggatcagcaa ctgacactca cggttgggga aaacggccac
    19621 tatacgctgg aaggtgaaga gttcaccgtc aatggtatgg tcggccagcg tctggaaaaa
    19681 gatggcgttg cgctgactat cgcggatatt aaggccaaac caggaacaca gtttgtcctg
    19741 agccaacgta ccgaactgga agcgattaac gcgttgcagg aaacctttac cgttagcgaa
    19801 cgcagtaaag aaagcgggat gctggaactt accatgactg gtgatgatcc ccagttgatt
    19861 actcgtattc tgaacagcat cgctaacaac tatttgcaac agaatatcgc tcgccaggcg
    19921 gcgcaggatt cacaaagcct tgaattctta cagcgccagt tgccagaagt gcgcagcgag
    19981 ctggaccagg cggaagaaaa actcaacgtt tatcgccagc agcgcgattc ggttgacctt
    20041 aacctggaag ccaaagccgt tcttgagcag attgtgaacg ttgataatca actcaatgag
    20101 ctgaccttcc gcgaggcaga gatctcccag ctgtataaga aagatcaccc aacttatcgt
    20161 gcgctgctgg aaaaacgcca gacgctggag caagaacgca aacgcctgaa taagcgggta
    20221 tcggctatgc cttccaccca acaggaagtg ttgcgtttaa gtcgtgacgt agaagcgggc
    20281 cgtgcggtat atctgcaatt acttaaccgc cagcaggagt tgagtatttc gaaatccagt
    20341 gccattggta acgtgcggat tatcgacccg gcagtcactc agccgcagcc agtgaaaccg
    20401 aaaaaagcgt tgaatgtggt gcttggtttt attcttggcc tgtttatttc ggtgggtgcc
    20461 gtgctggcgc gtgcgatgtt gcgtcgtggt gtagaagccc cggaacaact ggaagagcac
    20521 ggcatcagcg tttatgccac catcccgatg tccgagtggc tggataaacg tacccgtctg
    20581 cgtaagaaaa atttattttc taatcagcag cgccatcgta ctaaaaatat ccccttcctg
    20641 gcggtggata acccggcgga ttctgctgtg gaagccgtac gtgcgctacg aaccagtctg
    20701 catttcgcta tgatggagac tgagaataac attctgatga tcaccggtgc gacgccagac
    20761 agtggtaaaa cgtttgtcag ttcaactctg gcagcggtga tcgcccagtc cgatcaaaaa
    20821 gtgttattta ttgatgccga cttacgccgt ggttattcgc ataacctgtt taccgtgagt
    20881 aatgaacatg gcttgtcgga atatctggca ggtaaagatg agctcaacaa agtgatccag
    20941 cattttggca aaggaggctt tgatgtgatt actcgcggtc aggtgccacc taacccatct
    21001 gaactgctga tgcgcgatcg gatgcgtcaa ttactggaat gggcgaacga ccattacgat
    21061 ctggtgattg ttgatacgcc gccgatgctg gcggtgagcg atgctgcggt cgtggggcgc
    21121 tctgtcggca ccagcctgct ggttgcgcgt tttggcttga acaccgccaa agaggtgagc
    21181 ttgtcaatgc agcgtctgga acaggctggc gttaatatta aaggcgcaat cctcaatggt
    21241 gtgattaaac gcgccagcac cgcttacagt tacggctata actattacgg ttatagttac
    21301 tccgagaaag agtaaaactg aaggtaaagt gaccggatgc ggcgtgaacg ccttttccgg
    21361 tctacgcatt agacagttct tcgtttgtca tcagcctcag agcattcagg tagctgaatg
    21421 ctctttttta tacattacaa actgcacgcc ggtatgcgtg cttcattcac gatttgcgta
    21481 aaaccggcca acgaacacat gccctgcgca tttcgctctt cacatcctgc cagggtcagt
    21541 ttcacctctc cgggcggcgt atttaatgac agcggagttt tatcacgcat ctgctgtaaa
    21601 gtctggaaga ccagcgaaac ctgaatccac tggctgttat cgcttagccg acgccagcgt
    21661 tcaaacacca gttcaccacc tggcggcgtg ttatccggct gacctggaag cgtccagttg
    21721 agctccagtg cgccgccgag atttgccaga ttagtatcgt gtccggcgat aaacagcact
    21781 gaagtgggta atgtcacacc atacgcctgt ttttgcggtg gatggggagt caacgctatc
    21841 atgatcaaat ccaacaacgg ggtggcgcgg ctgcgggcaa cctctggcgt gcgttgtagt
    21901 aaataaaatt gcgcgttatg caaacttagc aacgtgttcc actggtgtga atcagtgatc
    21961 cttccccacc ccggctccgg cattccctgt gcctgttgca ggagaaatat ttccgtcagc
    22021 attgatgcga ggcttaccgc accggttaat gagacattgt cggcgctcac cttgagttcc
    22081 gatggtaatg cctgcgttaa tgaacagctt tcgtcctgtt tctcacggtt aaggcacaag
    22141 tttgattgcg gaaaattaag cacccgttcc agttcgcgaa acgccgtttg ccgatgcccg
    22201 gtaaagtcag caattgaccc tcctgccctg ctgaggatcg cgtcagtcac gttcgcgtta
    22261 tccagttggc aaacgccagt ttttagagga ttaaataacg gatcgggact ggacgtatct
    22321 gcctgggtat gtacggttat tgcacagtca ggtgccagcc cggcggcgaa ggcttcgcct
    22381 gttttacggg tacgctcgtc gacatcagca ataatcgcga cctgaccagg ctgcgggcag
    22441 ccctttttcg tcagcaatcc gtcggctacc agacgctggc gttggtaatg tccgagataa
    22501 gcgattagct caccaccgcg cggtgtcagc caacccagtt ttaccggcca gtttggccat
    22561 gcgtctgggg tgacatcctg catcagttgc gtggccttgg ttggggcacg cacaccatga
    22621 cgactgacaa tcaccacact ttccagcttc agctccggct ccggctcact ctgagcgaat
    22681 gcagattgcg gggttaacgg aatcagaaga gataaaaatg ggatcaagat cgctttcatc
    22741 gatatgtttc cgcttttcat atccgacatt attgattgcc tgatacgacg ctaatgcgcc
    22801 gcatcagact tacgttcgtt aacttttcaa tcgcgggtcc agccatacca acacaagggt
    22861 ggagagcgaa cacatcaaca aaatgccgac gaaccaaagt aaataccaca ttgttcctgc
    22921 tccttagtac agctcgtttt cgttacggcg gagagtttct gtcgtcatgc gcccccacat
    22981 tttgtagtag ctccagagag tgtagagcaa cacaatgggc aaaaatatca gcacgattac
    23041 caacataata ctcagcgtca gctgactgga agtgctgtcc cacaacgtca ggctggagat
    23101 tggactcacg cttgacggca tgacaaaggg gaacagcgtg atgcctgccg tgaaaatcac
    23161 gccaaattgc atcaatgacg ccatcaaaaa tccccaaccc gggcggccac gataaatcgc
    23221 catcaccgtc agcaatgggc agaagaaccc cagcagcggg aagatccaca aaacgggcga
    23281 ctcgacaaaa ttattcatcc aggcaccagg tagcactgcc accagtttca ttaacggatt
    23341 ggaaggaccg ttagcatcct gggcgagcag tacaaagcca tcaataccga cccacagcca
    23401 gtaacccgcc agcaaaaagc acagcatcac caacagtgca gcgcgtttag tcgacagctg
    23461 tgaacgcaga tgaatcacac caacagtttt cagttgtaac cagacgccac cttgcaaaat
    23521 caccatccca aggctgagca atccgcacaa taaagggaat ggcgtcagca gttgccagaa
    23581 gctgccgaga tactccacgc gtaattgcgg agtgaaggca aacggcacgc cgagcaacaa
    23641 gttgccgaag gcgataccga agactaccgg cggcaccaga ctgccgatga ccagaccggc
    23701 atcccacatt ttacgccagc gtgcatcggc gatttttccg cgataatcaa aggccagcgg
    23761 gcggaagaac agcgagcaca gcaccaggat catcgccaca taaaagccgg aaaacgccgc
    23821 tgcatacact ctgggccagg cggcaaataa tgccccacct gcgaggatca accagacctg
    23881 attgccttcc cagtgcgcac caacgctatt aatcactatc cggcgttcat catcatcacg
    23941 cgccaccagc ggcagcaaac agccaatccc catgtcaaat ccgtcggaga tcataaagac
    24001 caccaggatc acgccaatca gcagccacca gatgaagcgc aatgtttcat aatcaaacat
    24061 gattttctcc tttacccctg ttgctgcgtc ggttgttcac tctgcatcgc gctcggcccc
    24121 agacgggcat atttctgcat caggtagact tcggcgatta agaacagggt gtaaagccct
    24181 acgatcatga tcagtgagaa agccagttgt cctgtggtta aagcggagtg cgcggagtat
    24241 gtcggtaaga tgtcctgtat cgcccacggc tgacgaccaa actcggtcat aaaccacccg
    24301 gcttcaatcg caatccacgg caacggcaaa ctccagagcg ccattttcag cacccagcga
    24361 tgctggtcga ttttgccacg cagcgtctgg acaagcgcaa tcagcatcac cagtagcagc
    24421 agggaaccac agcccaccat gatgcggaaa ctccagaata ccggcgcaac ctgaggtatc
    24481 gcgccacgca tcgccgcctg gtactgtgcg gctgtgacat gattcatatc cggcgcatag
    24541 cgggagagca acatgccgta gcccaggtcg ccttccagct cccggaatgc ctgcaacaca
    24601 tgcggctcac gattgccttg cgatatttcc tgcattaaca gccaggccat acgtccgcgt
    24661 tgcaagcgtg ggtaggtttc agccatcaaa ttcttcagac ccggcacggg tttatctaat
    24721 gagtgagtgg cgaggatccc taacagcacg ggaattttga tggcaaaggc gttacgctct
    24781 tgatcctgtt ccggccaggc aaccacatgg aacggtgcag gagcaggttc cgtttgccac
    24841 tccccttcca tcgccgccag ttttaccggt tgtacttgcg cgacttcata cgcagaactg
    24901 tctccgagtt gcagggtacc gataatcgcc agagtaccga agacggaacc gatggcaaac
    24961 gagcgtaatg cgacatcgcg ctcccgtccg cgcagtaaat accaggcgct gatcgccata
    25021 ataaacatgg ccccggtcac gtagcccgcc attacggtgt gcacaaattt cacctggctg
    25081 accgggttaa agaccagctc gctgaagctg gtcatctcca tacgcagggt gtcgatatca
    25141 aaatgcgcac cggtcgggta ttgcatccaa ccgttggcat tcaatatcca caatgcagag
    25201 agatttgaac cgaacgccac cagccacgtc accagcaggt gctggtattt attcagccgc
    25261 tgccagccga agaagaaaag cccgacaaag gtggattcga ggaagaaggc cattaatgct
    25321 tccatcgcca gcggtgcgcc aaaaatatcg cccacatagt tggaatagaa tgaccagtta
    25381 gtaccaaact gaaactccat ggtcaggccg gtagccacgc caagagcaaa gttgataccg
    25441 aagagcttac cccagaagcg cgtcatatcg cggtagattg ttttgccggt gaccacgtaa
    25501 atggtttcca tgatagccag caaaaaaatc agccccaggg taaggggtac aaataaaaag
    25561 tgatacagcg cggtcagagc aaactgccag cgcgataaat caatgacatc ccacatgcgc
    25621 actcctgtag gcgaagagag aaaaataacc cgttaatggt agatctccct ctttatcctg
    25681 aaactctacg accagaggta ttcgccgatt gcgatattca taagcctgaa agcgatactt
    25741 accgtctttt tacgtcggtg cagcttccgc cagccactga catacctcac taagccgctg
    25801 cgcagagtcg accaaatctt ctggcgctgc cagcaccact tccggtattg ggcagatttc
    25861 ataactttcc agtaacgggc cttttaaggt gtccgtacag cgtaaatgcc agacatggcg
    25921 taaccccgtg gcgttgatat agctctcgcc atagccaatg gtacgaatct gaatatttcc
    25981 cggcccacag agacgtgaga gaaaaagccg atcagcctcg ctgatgggta attgcgtcag
    26041 attaatgctg tgcggctgcg cgtcggggtt acgtacatgc gccagtaact catgcgccaa
    26101 cggtaggcca ttcatcaggc catcaatggg cggcggtaac agcgaatctg tcggcaagag
    26161 attttgcgtt gccgcctgcc acaacgccag cggcgcgcag ccagcctcca gtttgtcctc
    26221 cagcaacttt tcgccgcgac gtctgcgcac ccgccataat ccgcagaaga tcgcctcctg
    26281 tatttcactt tcgctgtcgt cagcctgctg aatgcgcact gacacttcac cttccccgag
    26341 aagcgtattg ataaagtgac gatcgtcggc gttgagattt aacagatcga ctgccagcac
    26401 ctcgcccaac ggcggctggc gttgacttag ttgatgttgt aactcgttta acagcgcaat
    26461 cacctgcggg ctgtgagcac attgctccag ggccgccata ctcggttcat cattcacctg
    26521 acaggtgatc ggcagtggat tcatgctgaa actgtcatcg ttcggttgcg ttcttggccc
    26581 cagcagatgg aaaaaagttt cgctcatgag gcacgctcct gctgtggttc gacaagccca
    26641 cgcatcaggt ttatcagttc cgcccacggg tgaataccat tcagcacgcc gcgatagttt
    26701 ccgccggtaa acactaatgt ggcgggaaag cgaaagacgc caaagcgatc tccgatggct
    26761 tcgctctgtt caaggtcagc aatcgccacc tgccatgtgt agtcgggaaa ctcgcgcagt
    26821 aattcgccaa tcattaccgg attatcgctg acctctggcg tgcgtttcgg gtcactgctt
    26881 aataacacca cgccgtctgg cgcttgcgta agccagtcgt caagacggaa ttcactaacc
    26941 ggcgtccagc cgcgcgccag cattcgttgc cacaacgcat caaatggcgt gtcgttgctc
    27001 atccctgctc ctccagtgtc atcctgtact ggcgcaagcg aacgccttcg taattttcca
    27061 tcgacagaca gtcataattg agacagcgcg attcattagc cggttgcggc acaattcccc
    27121 atgcagccag ctgcgccagc gccgcctgtt ccgctgcggg cagttgctcc cgtgccagtt
    27181 cgctcaggct gccgccgtag tcgtcgagca ttgcgggttg cagaccgacg agggcaatat
    27241 gtgctggcag atgtccgcgg atatctgcca gcgccagcac ttcggagaaa ctgttctggt
    27301 gcaggctcat tttcttcgcg ctgagataag ccggaatacg ttctccggca taggttcgca
    27361 gcgttcctgg ttccagcccg tagtcaatgg catcgagaat caacagatgg ctggcgcttt
    27421 cgacataccc cagcaagttc agtccctgag taccgccatc gacaatctcc acagactcgg
    27481 gccagtggta atgggcatac aaccgttccg ccacccgcac gccgaaacct tcatcggccc
    27541 acagcaggtt gcccagcccc attaccacca cgcgttgctc gctcatgaac gctccttgtt
    27601 acttattttg ccaaatttgt ggctacggta gccgttgacc atggtggaga tcaccgtgtc
    27661 gtcggacatg atgtcttcac gcagcgccat atagacatga ccgatcacaa acgcgccaat
    27721 cagccacatc cccagccgat gccagctgtg aatgtccatt gagttgcctc ccgtccagta
    27781 gaaaaattcc accacataac ggaacggcgc aaaaatagcg tactggctgt gttcgctgta
    27841 cagcgcaaag ccggtgatga tcataaagac cgacatcagg aaatagccaa acatcgccgc
    27901 ctgggcgatg gggttatggc ctatatcggc actcggacgt tttgccagaa acagatacca
    27961 gcggatttca taccacacgc cctgccacca gcttttacgc catactggca cgataaacag
    28021 ctcgcgggag tatcgattgc caacaaaagc ccagtagatc cgcatcagca agaccacggt
    28081 aaaaaccatc ccggcgctga agtgaattaa cctgatgtag cccatataga acagatacgt
    28141 cgcctcgccg ctgacggaag gtagcggttt gccgataaag taaccggtga ccatcaacac
    28201 cgccatgcat aacaccgtca gccagtgcca gatacgcact ggcgcttcaa agacatagtg
    28261 gctgacaacg ttgtcgcttt tctgttgcat gatgattctc cttcgctgtt aacgcacctg
    28321 cacggagatc agctcgctac cgtcgtcgcc cagcacgtgt gttgaacagg cgaggcacgg
    28381 atcaaagctg tgcagagtac gcaggatctc cagcggttgc tcggggatcg ccattttggt
    28441 gttcatcagc gccgcttcat aagcgccaat ctgccctttg ggatcgcgcg ggctggcgtt
    28501 ccaggtggtc ggcaccacgc actggtagag atcaatcttg ccatcgcgaa tggcggccca
    28561 gtggcctaac gccccgcgcg gcgcttcggt aaaaccgaca ccacggcact ctgtcggcca
    28621 ggttgtgggt tcccattttt ccgtggaggc ggtggcaaga ttgccgtttt tcaggttagt
    28681 catcagcttg ttgaagaaat actgcaactt acctgcggcc cactgtgctt cgtgcgcgcg
    28741 gcacaaaatg cggcccaacg ttgactggat accggaaagc ggcaggttca gcgccgacat
    28801 catgcgatcg accgactcaa cggtcgcagc atcgcctttg tgataagcga ttaacgtgcg
    28861 tgccagcggc cccacttcca tcgcgttacc gcgccagcgc ggcgctttga tccacgagta
    28921 gcgttcctgt tcattcagct gctgaatgtt ggtatcgctg cctttgacat cgccggggtt
    28981 gtaccacggg tcggtgatgc catcgaacgg atgacgcccg acctgatcgt tgggataacg
    29041 ataccaggcg tgatcgacaa actcctgcac ctgctgcgga tcaaccaaat ccactggcag
    29101 cacattgttg aagtcgccgt taatcaccgc gccgccaggc atcagcagac ttttctcacc
    29161 aaagtcgttg gcaatatccg ggaatgcgcc gtagctgagg acacatttat cagaaagacc
    29221 cgtgccgatt tcgctccacg gtttgttgaa ctgaccgatg gctaaggcgt cggggatcat
    29281 cacgttgtta atgaagtctg ccgtgcgggt gataatcgac tgcaccaggt tcaggcgttc
    29341 catattgacc gccccgaccg cgccgctttc gtcaatgtta atggcgcaag gcatcccgcc
    29401 gacaatccag tttggatgcg ggtttttacc gccaaagacc gcgtggattt tgacaatttc
    29461 acgctggaaa tcgagagctt cgagatagtg ggcaaagccc atcaggttgg cttctggcgg
    29521 caacttgtac tgcgggtgcc cccagtagcc attgcggaag atccccaact gcccgccttc
    29581 aacgaatttc ttcaggcggt tttgtacgtc gaagaaatag ccaggggatg atttcggcca
    29641 agaggagaga ctttgcgcca gttcggaggt tttccgcggg tcggctttca gcgcatccaa
    29701 cacatcgatc cagtccatcc cggcaagctg atagaagtgc accagatgat cgtggcacca
    29761 gagcgttgcc agcataatgt tgcggatgat attggcgttg tccggcactt taataccgat
    29821 ggcatcttcg atggcgtaaa ccgaagccag agcgtgtacg ccagtacaga cgccgcagat
    29881 acgttcaacg aacgcccacg catcgcgcgg gtcgcgccct tgcaggatga tctccagccc
    29941 gcgaaacatg gtgccgcagg agacggcatt ggtgattaca ttctgatcgt taatattcac
    30001 ttcgcagcgc atgtggcctt caatgcgcgt aatcgggtcg accaccaggc ggcgtccggc
    30061 attattgatg gtgtatccct gagtttcgta ctgagtgctc atgcctgttt atcctcattg
    30121 cctggctgat gttcggtttc tgtaggttgc tgattatgac gtctgcgctg gtcaacggcg
    30181 ctggcgactg cgtgcacacc aacagccgct gccaccacac caagcgcggt taaaccgacg
    30241 gtatcggcgg tggaatgagt acccatttgt ggaatatcga ccacgcggct gtagaacgaa
    30301 ccgcgatccc agaaaccatt ttccgcacag cccaggcagc cgtgaccaga ctggattggg
    30361 aaagaaacgc catcattcca gcgtgtggag gaacaggcgt tataggtggt aggccctttg
    30421 cagcccattt tgtacaggca ataacctttg cgggcagcgt catcatccca actctggacg
    30481 aactctccgg cgtcgaagtg agcgcggcga tagcatttat cgtggatacg ctgaccataa
    30541 aacatcagtg gacgacccat tctgtcgaca tctggcaagc gatcaaaggt caccatgtaa
    30601 gtaatgatgg cgctcatcac atccgggatc ggcgggcagc caggtacttt gataatgggt
    30661 ttgtcggtga tgactttgtc gatagacgtt gcctgtgtcg gattaggtcg cgcggcctgc
    30721 acgcagcccc aggacgcaca ggttccccag gcgataatcg cgctggctcc ggcagcggca
    30781 cgtttgagtt tctcaataaa cggtcgaccg ctgctgatac agaacatccc ctgctcgccc
    30841 agcggcggat taccttctac tgcgaggata tatttgccat tgtattgcgt gatgatgtct
    30901 tcaaagactt cttccgcctg ggttccggcg gcagccatca aagtatcgtc gtaatcgagg
    30961 gaaatcaggg aaaggatgac gtcctttgcc agcgggtgag cggagcggat aaaagattcg
    31021 gtacagcagg tgcattccag accgtggatc cataccaccg gaatgcgcgg tttgttctcc
    31081 agcgcccagg caatctttgg tgccattccc gcgcctaatc ccagcgacgt ggcagccaga
    31141 ctacaatatt tgagaaagct gcgccgggta acgccctgac gccgcatggc ctggtaaaat
    31201 gtttcctcgt tattcatatc gcacgtctct tctccttgcg acaccggcag gacaccgggc
    31261 gcttatggtc ggcaagcgac gtccctgtag caaggcaaac catcgcttca cgtgagatgc
    31321 tgaaaacgaa agctcatcct tctgcactgg cgcacgtcgc cagaaagtat tgttaataaa
    31381 gcgtagtgaa acttttgcac aaaacaatac aaactgtgtg gatttatctt ttagcgataa
    31441 aaatggacct atttttcttt ttgccgggcg gtgggatgtt tagccagttg ctaaacgagt
    31501 aaaagagaag gaatttatgg tgatgaaatg atcaatctgc ttttatttca cgctatcaat
    31561 gcacttttgg ctgtttttca ggcaaacaaa cagattaggg gtttacacgc cgcatcggga
    31621 tgtttatagt gcgcgtcatt ccggaagtgt ggccgagcgg ttgaaggcac cggtcttgaa
    31681 aaccggcgac ccgaaagggt tccagagttc gaatctctgc gcttccgcca aataatcaag
    31741 gggttaccaa atggtagccc ctttgctttt ttggctcttg gtataaactt ggtataccaa
    31801 ttggtatatt tttattcgga tgagattatc ggtcatgagc ggaacctcag gttcctcgtc
    31861 tgatgccgct cttgcaacac gttacgctgc cgagtatttt tgtaaaacat ggactgctcc
    31921 aggcctgaac caggcagaag gatacaaagc catctcagat ctgtcgcatc actattttcg
    31981 tgcagaaggc tctagccctc cccaatcttt tttgtgacac cctgtttcac tgaacatgaa
    32041 gacactcacc ttgtccctga tattcattag catcatcctg attatttaac ttctctattt
    32101 agggaagccc gtctgataca aagcttctat tcctcgttaa atattctcca ttctggcgcc
    32161 aagccctatc tatagctgat tgaaaacaac tctaaaaacg taataatatc aaatagttta
    32221 ttcctacagc cgatcatttt gagtaaaata ttgacgagaa tttcacaaaa ggtagatttc
    32281 atgtaaacgg aaataagact atccaataaa gctcttataa ggttatctat atgataaata
    32341 gcattaattc tttttttccg gccttcctcg cagtatatca agtgctatac gcagcagtac
    32401 cttcactgtc tcgcagcaca aaagcacacc taacacggta aaaaccagct cacctttttc
    32461 tcccagcaac agtcctgcca gtgcaacaac tatttttaag gtaaaaaatt cttatacaga
    32521 atcaggactt caacgtccca catcttatac tcagtcaagt atagaaaaaa acgctttaca
    32581 tcggcctcta cctgatgt
//
LOCUS       DABSYT020000046        32477 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.046, whole genome shotgun sequence.
ACCESSION   DABSYT020000046 DABSYT020000000
VERSION     DABSYT020000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.046"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            52..654
                     /gene="yebB"
                     /locus_tag="ID725_RS12860"
                     /old_locus_tag="pgaptmp_002572"
     CDS             52..654
                     /gene="yebB"
                     /locus_tag="ID725_RS12860"
                     /old_locus_tag="pgaptmp_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGV7851149.1"
                     /translation="MNINYPAEYEIGDKVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(656..1177)
                     /gene="ruvC"
                     /locus_tag="ID725_RS12865"
                     /old_locus_tag="pgaptmp_002573"
     CDS             complement(656..1177)
                     /gene="ruvC"
                     /locus_tag="ID725_RS12865"
                     /old_locus_tag="pgaptmp_002573"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGV7851150.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1212..1952)
                     /gene="yebC"
                     /locus_tag="ID725_RS12870"
                     /old_locus_tag="pgaptmp_002574"
     CDS             complement(1212..1952)
                     /gene="yebC"
                     /locus_tag="ID725_RS12870"
                     /old_locus_tag="pgaptmp_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGV7851151.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1981..2433)
                     /gene="nudB"
                     /locus_tag="ID725_RS12875"
                     /old_locus_tag="pgaptmp_002575"
     CDS             complement(1981..2433)
                     /gene="nudB"
                     /locus_tag="ID725_RS12875"
                     /old_locus_tag="pgaptmp_002575"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGV7851152.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2551..4323)
                     /gene="aspS"
                     /locus_tag="ID725_RS12880"
                     /old_locus_tag="pgaptmp_002576"
     CDS             complement(2551..4323)
                     /gene="aspS"
                     /locus_tag="ID725_RS12880"
                     /old_locus_tag="pgaptmp_002576"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGV7851153.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4633..5199
                     /gene="yecD"
                     /locus_tag="ID725_RS12885"
                     /old_locus_tag="pgaptmp_002577"
     CDS             4633..5199
                     /gene="yecD"
                     /locus_tag="ID725_RS12885"
                     /old_locus_tag="pgaptmp_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGV7851154.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5196..6014
                     /gene="yecE"
                     /locus_tag="ID725_RS12890"
                     /old_locus_tag="pgaptmp_002578"
     CDS             5196..6014
                     /gene="yecE"
                     /locus_tag="ID725_RS12890"
                     /old_locus_tag="pgaptmp_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGV7851155.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6067..6462
                     /gene="yecN"
                     /locus_tag="ID725_RS12895"
                     /old_locus_tag="pgaptmp_002579"
     CDS             6067..6462
                     /gene="yecN"
                     /locus_tag="ID725_RS12895"
                     /old_locus_tag="pgaptmp_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGV7851156.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6503..7246
                     /gene="cmoA"
                     /locus_tag="ID725_RS12900"
                     /old_locus_tag="pgaptmp_002580"
     CDS             6503..7246
                     /gene="cmoA"
                     /locus_tag="ID725_RS12900"
                     /old_locus_tag="pgaptmp_002580"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGV7851157.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7243..8214
                     /gene="cmoB"
                     /locus_tag="ID725_RS12905"
                     /old_locus_tag="pgaptmp_002581"
     CDS             7243..8214
                     /gene="cmoB"
                     /locus_tag="ID725_RS12905"
                     /old_locus_tag="pgaptmp_002581"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGV7851158.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8379..10808)
                     /gene="torZ"
                     /locus_tag="ID725_RS12910"
                     /old_locus_tag="pgaptmp_002582"
     CDS             complement(8379..10808)
                     /gene="torZ"
                     /locus_tag="ID725_RS12910"
                     /old_locus_tag="pgaptmp_002582"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGV7851159.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10833..11933)
                     /gene="torY"
                     /locus_tag="ID725_RS12915"
                     /old_locus_tag="pgaptmp_002583"
     CDS             complement(10833..11933)
                     /gene="torY"
                     /locus_tag="ID725_RS12915"
                     /old_locus_tag="pgaptmp_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGV7851160.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLMAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(12321..13067)
                     /gene="cutC"
                     /locus_tag="ID725_RS12920"
                     /old_locus_tag="pgaptmp_002584"
     CDS             complement(12321..13067)
                     /gene="cutC"
                     /locus_tag="ID725_RS12920"
                     /old_locus_tag="pgaptmp_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGV7851161.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(13081..13647)
                     /gene="yecM"
                     /locus_tag="ID725_RS12925"
                     /old_locus_tag="pgaptmp_002585"
     CDS             complement(13081..13647)
                     /gene="yecM"
                     /locus_tag="ID725_RS12925"
                     /old_locus_tag="pgaptmp_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGV7851162.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13863..15596
                     /gene="argS"
                     /locus_tag="ID725_RS12930"
                     /old_locus_tag="pgaptmp_002586"
     CDS             13863..15596
                     /gene="argS"
                     /locus_tag="ID725_RS12930"
                     /old_locus_tag="pgaptmp_002586"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGV7851163.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(15638..15682)
                     /locus_tag="ID725_RS12935"
                     /old_locus_tag="pgaptmp_002587"
     CDS             complement(15638..15682)
                     /locus_tag="ID725_RS12935"
                     /old_locus_tag="pgaptmp_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851164.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            15773..16249
                     /gene="yecT"
                     /locus_tag="ID725_RS12940"
                     /old_locus_tag="pgaptmp_002588"
     CDS             15773..16249
                     /gene="yecT"
                     /locus_tag="ID725_RS12940"
                     /old_locus_tag="pgaptmp_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGV7851165.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWLDFRD
                     KECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLTRG"
     gene            complement(16369..16761)
                     /gene="flhe"
                     /locus_tag="ID725_RS12945"
                     /old_locus_tag="pgaptmp_002589"
     CDS             complement(16369..16761)
                     /gene="flhe"
                     /locus_tag="ID725_RS12945"
                     /old_locus_tag="pgaptmp_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGV7851166.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(16761..18839)
                     /gene="flhA"
                     /locus_tag="ID725_RS12950"
                     /old_locus_tag="pgaptmp_002590"
     CDS             complement(16761..18839)
                     /gene="flhA"
                     /locus_tag="ID725_RS12950"
                     /old_locus_tag="pgaptmp_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGV7851167.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(18832..19980)
                     /gene="flhB"
                     /locus_tag="ID725_RS12955"
                     /old_locus_tag="pgaptmp_002591"
     CDS             complement(18832..19980)
                     /gene="flhB"
                     /locus_tag="ID725_RS12955"
                     /old_locus_tag="pgaptmp_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGV7851168.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(20182..20826)
                     /gene="cheZ"
                     /locus_tag="ID725_RS12960"
                     /old_locus_tag="pgaptmp_002592"
     CDS             complement(20182..20826)
                     /gene="cheZ"
                     /locus_tag="ID725_RS12960"
                     /old_locus_tag="pgaptmp_002592"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGV7851169.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(20837..21226)
                     /gene="cheY"
                     /locus_tag="ID725_RS12965"
                     /old_locus_tag="pgaptmp_002593"
     CDS             complement(20837..21226)
                     /gene="cheY"
                     /locus_tag="ID725_RS12965"
                     /old_locus_tag="pgaptmp_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGV7851170.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(21241..22290)
                     /gene="cheB"
                     /locus_tag="ID725_RS12970"
                     /old_locus_tag="pgaptmp_002594"
     CDS             complement(21241..22290)
                     /gene="cheB"
                     /locus_tag="ID725_RS12970"
                     /old_locus_tag="pgaptmp_002594"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGV7851171.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(22293..23153)
                     /gene="cheR"
                     /locus_tag="ID725_RS12975"
                     /old_locus_tag="pgaptmp_002595"
     CDS             complement(22293..23153)
                     /gene="cheR"
                     /locus_tag="ID725_RS12975"
                     /old_locus_tag="pgaptmp_002595"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGV7851172.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(23172..24773)
                     /gene="tap"
                     /locus_tag="ID725_RS12980"
                     /old_locus_tag="pgaptmp_002596"
     CDS             complement(23172..24773)
                     /gene="tap"
                     /locus_tag="ID725_RS12980"
                     /old_locus_tag="pgaptmp_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGV7851173.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(24819..26480)
                     /gene="tar"
                     /locus_tag="ID725_RS12985"
                     /old_locus_tag="pgaptmp_002597"
     CDS             complement(24819..26480)
                     /gene="tar"
                     /locus_tag="ID725_RS12985"
                     /old_locus_tag="pgaptmp_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGV7851174.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(26623..27126)
                     /gene="cheW"
                     /locus_tag="ID725_RS12990"
                     /old_locus_tag="pgaptmp_002598"
     CDS             complement(26623..27126)
                     /gene="cheW"
                     /locus_tag="ID725_RS12990"
                     /old_locus_tag="pgaptmp_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGV7851175.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(27147..29111)
                     /gene="cheA"
                     /locus_tag="ID725_RS12995"
                     /old_locus_tag="pgaptmp_002599"
     CDS             complement(27147..29111)
                     /gene="cheA"
                     /locus_tag="ID725_RS12995"
                     /old_locus_tag="pgaptmp_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGV7851176.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(29116..30042)
                     /gene="motB"
                     /locus_tag="ID725_RS13000"
                     /old_locus_tag="pgaptmp_002600"
     CDS             complement(29116..30042)
                     /gene="motB"
                     /locus_tag="ID725_RS13000"
                     /old_locus_tag="pgaptmp_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGV7851177.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(30039..30926)
                     /gene="motA"
                     /locus_tag="ID725_RS13005"
                     /old_locus_tag="pgaptmp_002601"
     CDS             complement(30039..30926)
                     /gene="motA"
                     /locus_tag="ID725_RS13005"
                     /old_locus_tag="pgaptmp_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGV7851178.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(31053..31631)
                     /gene="flhC"
                     /locus_tag="ID725_RS13010"
                     /old_locus_tag="pgaptmp_002602"
     CDS             complement(31053..31631)
                     /gene="flhC"
                     /locus_tag="ID725_RS13010"
                     /old_locus_tag="pgaptmp_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGV7851179.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(31634..31984)
                     /gene="flhD"
                     /locus_tag="ID725_RS13015"
                     /old_locus_tag="pgaptmp_002603"
     CDS             complement(31634..31984)
                     /gene="flhD"
                     /locus_tag="ID725_RS13015"
                     /old_locus_tag="pgaptmp_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGV7851180.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         7688 a         8780 c         7882 g         8127 t
ORIGIN      
        1 aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
       61 aattaccctg ctgaatatga aattggagat aaagtcttta catgtatagg tgctgcctta
      121 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
      181 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
      241 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
      301 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
      361 ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt
      421 gatatttata aagaagcgct atgtattccg gtgggtgaaa tagagacgtt tggagaattg
      481 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttcct gggttctatt
      541 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccagg tttggtgttg
      601 attcatgcgg agggagtgga aacgcctcag ccggaactga ccgaggcggt ataacttaac
      661 gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcatcgca ttctgactaa
      721 cgtggcagtg ggtgatggca atcgccagcg catcggcggc atccgcctgt ggattagcgg
      781 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac
      841 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa tactggcaac tcctgattca
      901 ccgccgccac aatcgccacg ccgcgcgcct ggcccagttt cagggctgag tcagcgttct
      961 ttgccataaa gacttgttca atggcgaaat aatcgggctg gaactgggtg atgatttccg
     1021 tcacgcccgc atagatgagt ttcagacgag acggtaaatc atccactttg gtgcgtatgc
     1081 atccgctacc caggtaggac agttgcctac ctacctggcg gatgacgccg tagccggtca
     1141 cgcgcgaacc cggatcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
     1201 agcaggcctc atcagagagt cgctgcgacc tcatcagaga tttcaccgtt atggtaaact
     1261 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt
     1321 tctgcatcca tatcagcttt ggtagacggg atcatggaaa cttccgcgct gtctgctttc
     1381 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagaca
     1441 tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc
     1501 atgatggtgt cttcatcgcc tttctcgaag gagatgacgc cttttttgct gaacagatag
     1561 gctacggaac catcagtacc gaggttaccg ccacatttgc tgaatgcatg acgcacttca
     1621 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
     1681 ccgtaacctt cgtagatgat ggtttccatg ttcgcatcat catcaccgcc cacaccacgt
     1741 gcaattgcgc ggttcagagt gtcacgggtc atgttgttag acagtgcttt atcaattgct
     1801 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
     1861 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
     1921 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
     1981 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
     2041 cgccgccgca gcaggcgcat caagccactt gtaagccaga tgttcagtga aaacgatctg
     2101 ccgctcatgc ggaagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
     2161 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
     2221 ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc
     2281 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcggggtc
     2341 atcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcgtagatga ccactaagat
     2401 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
     2461 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg cctcgatgca
     2521 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
     2581 aatgctcagc tcagccagtg cagccgggtt agcaaagctc ggtgcttcag tcatcagaca
     2641 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccggtcag
     2701 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
     2761 cagagcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
     2821 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
     2881 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
     2941 ctctgcagcc gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
     3001 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
     3061 gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac
     3121 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
     3181 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
     3241 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
     3301 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
     3361 cagagcggca acgcgacctt tcggatcgtt cgccggacct gcaaatactg caaactcaac
     3421 agatctcagc agatcagcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
     3481 agaaccataa cggcgttcgg cttccgcaaa ggtcattact gggaaatcgc ccagatccac
     3541 gcccttcact tccagccaca gatggcgcac cagcgcttcc atcacttcac gcacttgcgg
     3601 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
     3661 caggtcttcg tcacggaagc atttaacgat ctgatagtag cggtcaaagc cggacatcat
     3721 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
     3781 acgagaaggc accaggtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
     3841 ttcgatgtcg aggaaaccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
     3901 gcgggttttc aggcgctgag ccatttccgg acgacgcagg tcgaggtagc ggtatttcag
     3961 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
     4021 gatagtcagc gaggacgcca gcacttcgat ttcgccggtc gccatatcgc ggttaatatt
     4081 tttttcgtca cgcgcacgta cggtgcccgt gacctgaatg cagaactcat tacgcagttc
     4141 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
     4201 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
     4261 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
     4321 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
     4381 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
     4441 tgccgcaccg taagagcctg gcccgcgctg gaacgcctcg ttaccacttt atatcgggcc
     4501 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat acaaattaac
     4561 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
     4621 tcaggagtca tcatgcttga acttaatgct aaaaccactg cgctggtggt gatcgattta
     4681 caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc
     4741 gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgcgtt
     4801 ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ttcccccgct
     4861 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
     4921 atcgaaataa tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaatta
     4981 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggagttgaa
     5041 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agatgcctgt
     5101 agtgccgcta gcgccgagca gcacaataac agtatcaacc atatctaccc gcgcatcgcc
     5161 cgtgtgcgta gcgtggaaga gatcctcaac gcgttatgat ttacatcggt ctaccgcaat
     5221 ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgtcact
     5281 ttaactgcgt ggagggcaac accacgcttt acgccctgcc gaaacccgag gttgtcctgc
     5341 gctggcgtga acagaccaca gatgacttcc gcttctgttt taagtttccg gcgaccattt
     5401 cgcatcaggc agcattacga cattgcgatg atttagtgac tgaatttttg acccgcatgt
     5461 caccgttggc tccgcgcatt gggcaatact ggctgcaact gcctgccaca ttcggcccac
     5521 gggagctgcc tgcgctttgg cattttctcg attctctccc tggcgaattt aattatggtg
     5581 tggaagtccg ccatccacag tttttcgcca aaggggaaga ggaacaaacg cttaatcgcg
     5641 gtttacatca gcgcggcgtt aatcaggtga ttttagacag ccgcccggtt catgcagcac
     5701 gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg
     5761 ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat atgacgcaaa
     5821 accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag accactacgc
     5881 cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta cataccctgt
     5941 gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt ccacagcaat
     6001 cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat tttaaaggga
     6061 gtttgtatgg taagcgcgct gtatgccgtt ttaagtgcgt tgttattaat gaagttctct
     6121 tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg tggttttagc
     6181 gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc catcgcgatt
     6241 gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca tatttgcggc
     6301 atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg tctgttccgc
     6361 tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat
     6421 ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat acgccacttt
     6481 ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg cccctatcgc
     6541 cagactgggc gactggacct ttgatgaacg ggtagctgaa gtcttcccgg atatgatcca
     6601 gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag ccgagcgctt
     6661 cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg cgacgctctc
     6721 ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca actccccggc
     6781 gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc cagtagacgt
     6841 cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg tgctgaattt
     6901 taccctgcaa ttcctggaac cttccgagcg ccaggcgtta ctggataaaa tttatcaagg
     6961 gctgaacccc ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag atgccaaagt
     7021 tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt acagcgaact
     7081 ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt ccgtggaaac
     7141 ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt tccagtgctt
     7201 taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga ctttggtaac
     7261 ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct gcccgcgcag
     7321 attgctaact ggcagcgcga gcagcagcac gggttgttta agcagtggtc aaatgcggtg
     7381 gagtttctac ctgaaattaa accgtatcgt cttgatttat tgcatagcgt tacggcagaa
     7441 agcgaagagc cactgagcgc agggcaaatt aaacgcattg aaacgctgat gcgcaacctg
     7501 atgccgtggc gcaaagggcc gttctcactg tatggcgtca acatcgatac cgaatggcgt
     7561 tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg acgcaccatt
     7621 ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc aggcgcgcac
     7681 ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc ggtgcgtaaa
     7741 ctactgggta atgatcagcg cgcgcatttg ttaccgttag gtattgaaca acttccggca
     7801 ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt atcatcgtcg ttcaccgctg
     7861 gagcatctct ggcagttgaa agatcaactg gtcaatgaag gtgaactggt gcttgaaacg
     7921 ctggttattg atggcgatga gaacacggtg ctggtgccgg gcgatcgtta cgcgcaaatg
     7981 cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa gaagtgtggt
     8041 tttgtagata tccgcattgc ggatgtgagc gttaccacta cagaagagca gcgacgcact
     8101 gaatggatgg ttaccgagtc tctgtccgat ttcctcgacc cacatgatcc gagtaagaca
     8161 gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc gtaaaggtct
     8221 ggtattactg ccggatgcgg cgtgaacgcc ttatccagcc tacaaaacct tgctaattca
     8281 atatattgca gggactatgt aggcctgata agcatagcgc atcaggcagc tttatgtttg
     8341 cataacctca gcgcccgttt ccgggcgctt ttcacgtctt atgcctgaac agctggctga
     8401 tcaaacgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac cagcgcagag
     8461 ttaccggcac aggcatttgc cagctgcgag ctggggatat ccgccgttaa cacgttcgca
     8521 ctgccgtttt tacacaagcc attttccaga tctggccatg caccttcatg cacgcatacc
     8581 acgccttttt tgatcccgtc agtcaccaca gcgcctgtca aaatctgacc acgtttgttc
     8641 cagacacgga ccagatcgcc attcgcaata ccaaagcgag cagcatcttc ggtgtgaata
     8701 gtgattggtt cacgatcggc aaccgcatat tttttacgca gttgcgcata gttaagctga
     8761 ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgta actgcttctc gtcggcggta
     8821 cctttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt atagccaaat
     8881 ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag cgcatttttc
     8941 accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc gctgcggcgc
     9001 atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt gacgcgttgc
     9061 gcacgggcac ctttctgagc ggcatcatag aaaaatttca gccacgccat ttcatcttta
     9121 ccttcggtat agatctcttt tccgcctggt ttcagtaatt ccgcaagttc ggcaaacacg
     9181 tcaaaatcgt ttcgcgcttc aaattgcgga gcgacagcct gcttcatcgg tacaatatgc
     9241 tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt ggtgatcggt
     9301 aatacgatat ctgcatgttt agcagctgcg gtccagtagc attcagaaac gacgatcatc
     9361 tccggtttct gccatgcttt gatcagacga ttagtatcct ggtgatgggt aaagttaccg
     9421 ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc gttatgctga
     9481 tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat cgccgtcatt
     9541 ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat ttccggcagc
     9601 acgccgccaa cacgcgtcgg gttgccaccg ttggagtaat gataagagaa accaaaaccg
     9661 ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat ccagtgtttc
     9721 tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt gcgattcgcc
     9781 gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat ttccgccgtc
     9841 caggccgcgc tcttcggagt attatcgctc ttacctgtca gatactcttc aaactgcgga
     9901 tacccggtgg tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt catcagggta
     9961 tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca
    10021 ttatagccaa agaactcgat ggtttcagag cggataggat caatggcaat cactggtttg
    10081 ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact gctccaggca
    10141 atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct gttttccaga
    10201 atcagcggcc aggaggtctg ctgttcatac acctctacag aaccgaccac atgcggcatg
    10261 atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc gcccgccagg
    10321 ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg
    10381 taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc atgttgctca
    10441 tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc gccacgacct
    10501 ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg ctgaatacgc
    10561 gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat ggttttcgcc
    10621 agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgttcat cgctccccaa
    10681 cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcgggtag cggtgctgcc
    10741 gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc acgtcttgtt
    10801 aatgtcataa cttcctccct gatcaacgag gatcactgtt tctcggtaat atctttggcg
    10861 ttgtactgga aataccgcgt taaaatgtcc agttcgtttt cgctcatgct ggttcgtgcc
    10921 cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc ggcaataggg
    10981 gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca tagcagttta
    11041 cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg ccattgattg
    11101 ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc ttcttcactt
    11161 aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc actgccctgc
    11221 atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac gacggtgaga
    11281 tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa aggataaata
    11341 tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc cgcgttgtca
    11401 tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat acaggtttcg
    11461 ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga ggcaatatcc
    11521 atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt taattctttc
    11581 catactgttt cggccatttc ctggcgatga gcttcgaact tatcgtcact gtctattttg
    11641 ccgctaacaa attcatgata aatatcttta gatgctttta atttagcgaa taaataatcc
    11701 atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt ctggttcgaa
    11761 aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca ggaaaggcaa
    11821 aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca tcaagaaccc gccaccacca
    11881 accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg catagattcc
    11941 tctaattaaa ctccagaaca ccgaaatcat tgtataaata aatacacaac gaattgacta
    12001 cttgcttaat tcggcacaac actgcctgaa ataatcgcta aagaatatga tttattacaa
    12061 tgtaatcatt aattgctaag gaataatcca gttgttattt agaatatata tttgtcagtt
    12121 ttagcaattt aaatcacata attaatgtga atatataaaa tatttttagc acaacgctat
    12181 gtgcactgtc acccgctcaa cagatcgcgg taatcgttca ttttttatac tattcaggga
    12241 taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc atattgggcg
    12301 acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc tttcatttca
    12361 gcaaccgccg ccccgtctac gacataacgc gaatactcgt ccgcgtgtgc atctgatgac
    12421 atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc cgcggagcta
    12481 tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg gactcctgct
    12541 ccggccataa tgattggagc atcacgatgg gcaataagtt ccataatttt tgataaacct
    12601 tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa ttccgcaaga
    12661 ttattgagtg tatttagagg gttagcgcac atatcaaagg cgcgatgaaa agtcactgcc
    12721 agcggaccgg cagcagccat tattttttcc attcgtgaca tatcgacatt accgtcaaca
    12781 tcgagcacgc ccgtcacgag tccaggaaaa cctaattcgc ggaccgtgcg cacatcctca
    12841 agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg gcgaattatc
    12901 ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacacccaa cgacggcgtt
    12961 aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt ttgctgcgcc
    13021 gttagcgcgc attccatgct gtaacagcaa atttccagta atgccatttt tgctccttaa
    13081 ttacgccgac tgctcgctgg caacaatctc ttcaatggac cacggatgaa atttaatggt
    13141 cgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgttcatgct caccttttgg
    13201 cgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa gcaatgccag
    13261 cgcacgggca ttcagcgttt ctggatcacc cggcaaaacg atttcaatat gttcccaacc
    13321 ttcgtgcggg taacgttttt ccctaggcca cggtagctcc acaatagaaa actgccagtg
    13381 tgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat tgatcatatt
    13441 ttctgacaaa agctcgccac actgttcaaa cccgcgacgc cagcgctcag cagtggcgtt
    13501 ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatattca accccagacg
    13561 gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa tatcctgcag
    13621 ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa aagcgttaat
    13681 ttaccctgtt gccctgcgcc aaccaaccgc tgatttcacg ccgcttctga tgcaatagtg
    13741 aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca gtatactccc
    13801 gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg taaggtattc
    13861 cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt gcggcaggcg
    13921 cgcctgcgga ttgcgaaccg caggttcgtc agtcagcaaa agttcagttc ggcgactatc
    13981 aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga caattagcag
    14041 agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag atcgccggtc
    14101 caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt cagcaggcgc
    14161 tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg gttgactact
    14221 ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc attattggtg
    14281 acgcagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca aaccacgtcg
    14341 gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag cagcaggaaa
    14401 acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt
    14461 acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg
    14521 gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg acgcagaacc
    14581 agatcactta cgatcgtctc aacgtgacgc tgacccatga tgacgtgatg ggcgaaagcc
    14641 tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt ctggcagtag
    14701 aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa ggcgaaccga
    14761 tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact gatatcgcct
    14821 gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac atcgactccc
    14881 gtcagcatca acacctgatg caggcatggg ctatcgtccg taaagcaggc tatgtaccgg
    14941 aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac ggcaaaccgt
    15001 tcaaaacccg cgcgggtggt acagtgaaac tggccgatct gctggatgaa gccctggaac
    15061 gtgcacgccg tctggtggca gaaaagaacc cggatatgcc agccgacgag ctggaaaaac
    15121 tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa aaccgcacca
    15181 cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc gcgccataca
    15241 tgcagtatgc atacacgcgt gtattgtccg tgttccgtaa agcagaaatt gacgaagagc
    15301 aactggctgc agctccggtt atcatccgtg aagatcgtga agcgcaactg gcagctcgcc
    15361 tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg catgtaatgt
    15421 gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac tgcccgatcc
    15481 tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa ctgacagcga
    15541 agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt atgtaatcga
    15601 tttttcgtga gagtgaagcc tgatcaggtt tagccgatca ggctttttta ttaccatcta
    15661 aatgtattct gaaaataggc atgccattgt tttctcgctg ttgagtaaga ggccagaagc
    15721 gtaatatcca gctccaggga aacggtaacg gttcaattta aggaataccg cagtgtttaa
    15781 atttcttgta ttaacattag gcattatctc ttgccaggtt tacgcagaag atacgcttat
    15841 agtaaatgat catgacattt cagccatcaa agattgttgg caaaaaaatt caggtgatga
    15901 tactgacatt aacgtgatca aatcatgtct gcgacaagaa tacaatctcg tcgatgcgca
    15961 attaaataaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacctga
    16021 tactgaacaa cttaacttgc ttaaaaaatc acaacgagcc tggctggatt ttagggacaa
    16081 agaatgtgaa ttaatccttt caaatgagga cgttcaggat ttaagtaacc cttattctga
    16141 atcagaatgg ctatcatgta tgatcataca gaccaatacg cgaactcgcc agttgcagct
    16201 ataccgtaac tctgaagatt tttatccaag ccctttgaca agaggataat tcacatattt
    16261 ttggcatgtt ttgttgcaag ctattcctga taaatgattg caacaaaaca tccagccttt
    16321 ttcactgagt tattaaacat actcgcgagc gcgtaatttt tttgtccttc agcgataatt
    16381 cacaatcact tcattacgtt gtactttcag cgacggaatt aaccgcccac cgccaggcac
    16441 ttcccagata aagcgcaacg gttctgctgc cgctataccg gaaaaggcca cggtggttcc
    16501 gctctgccca tctaattcga cacaacgaga ttgcgaacac aagcgaaccc gcagtcctgc
    16561 aggtgtcggg ccgataagct gataccgcca cgccaccagc gtcatcaatc ctgaagcagg
    16621 ttgtcgcgta gaaagaggtg cagacgacat cgactcaccg cgatgattta gcgtaattcc
    16681 gacactactt gcctgccaca tcccctcccc ggcggcttgc accagcagcg gaaacaataa
    16741 tattgctaat aaggttctca tcatttaccg ccaattgtcg ccgtcatgcg gatatgtcgg
    16801 ttatcagaca gttccagatt cgacagcacc actaactgcg gcaagctgcg gcgcagaaag
    16861 cgagacaata atggtcgcag cgcgtggttc accaataaaa ctggcggcgc acccaacatc
    16921 tcctgacggg atagcgcttc ctgagtttgc gccagtaaac gatccgccag ccctggctcc
    16981 agtccgcccc cgccctgcaa cgcctgtagc aacaaacgtt ccagcggtgt atcgaggcca
    17041 ataacatgga cttcatcttt gccaggaaac cactgctggg taatcgcccg tcccaacgcc
    17101 acgcgcacga cggcggttaa ttcatgtgga tcgctttgga tgggcgcatg ttccgccagc
    17161 gtttcgagaa tagtgcgcat atcgcgaatc ggtacttttt catcgaggag attttgcagc
    17221 actttatgca gtgtggtgag cgtgacgacg ccagggacga gatcttccgt cagctttggc
    17281 atctcctggg cgacgcgatc cagcagctgt tgcgcctcct gacgaccaaa cagctctgcg
    17341 gcatgctggc taatgaggtg gttaagatgc gttgctacca ccgtgctggc ctcaaccact
    17401 gtgtacccct gaatctgcgc ctgttctttt agcgcacttt caatccagat agcattcagg
    17461 ccaaatgccg gatcgacggt cgcctcacca ggtaacgtcc cggcagcggt tccagggtta
    17521 atagccagcc agcgccccgg ataagcatca ccactgccta tctccacgcc tttcatcaaa
    17581 atgcgatagc gggcaggttg cagatccata ttgtcgcgaa tgtgcaccac tggcggcaga
    17641 aatcccatct cctgggcaaa tttcttgcgg atactgcgta tacggcccaa caactcacca
    17701 tcctgctgga aatcgaccat cgggatcagt cgataaccca cttccattcc cagagaatct
    17761 tccagttgta catcgttcca cgtcgcttcg acaacggcat tattctctgc catttttacc
    17821 ggttttggtt cggcaggcgc tttttgttcg cgtccacgga tccaccaggc cagccccagc
    17881 aatccggcag tgaacagcaa aaataccagg ttcggcattc caggcaccag gccgagtaaa
    17941 ccgagcacgg cagcgcttaa caacataacg cttgggttac tgaaaagctg attcaccatc
    18001 tgctcgccaa catcctgatc ggtgctgaca cgcgtaacaa tgacccccgc ggcggtagaa
    18061 atcaccagag ccgggatttg tgccaccaga ccgtcaccaa tggtcaagag cgtataactt
    18121 tccgccgcgt gtcccatgct catgccatgt tgcagcacgc cgaccagcaa cccgccgaca
    18181 acgttaatga ccatgatgag gatcccggcg atggcatcgc cgcgaacaaa cttacttgcc
    18241 ccgtccattg agccgtaaaa atcggcttcc tgagtgactt cggagcggcg ttttttcgcc
    18301 tcatcttcac caatcaatcc ggcgttaagg tcggcgtcaa tcgccatctg cttacccggc
    18361 ataccatcga gaacaaagcg cgcacccact tctgcgatac gcccggcacc tttggtaatg
    18421 accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc gaaattgcca
    18481 ccaacgagga agtgaccgaa cgcttcaacc accttccctg ccgccgctgc gccggtatgc
    18541 ccttccatta aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa cagcgtggta
    18601 aacaacaaaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa catcgccacc
    18661 agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag tatgaatgcg
    18721 ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc ggcaaggatc
    18781 tgccattgtg tcgatttcag gtttgtgggc aggcgcagca tcgcggccag attactcatg
    18841 ggtcggtttc tcgttaataa aatccagggc ttccggcacc ggaagatgag taggttgtac
    18901 agggcgctgt ccaccagcca gacgccagcg tttcagttgc cagacccagg ccagcacttc
    18961 cgccaccgcg gcgtacagtt gacccgggat ttgttgacca atctccacat gtcgatacag
    19021 cgctcgcgcc agcggcggcg cttcaagcgt cgggacgtta ttttcagcgc caatttcacg
    19081 aatgcgcagc gcgaccagcc ctgcaccttt agcgaccact ttcggtgcgc tcattttgtt
    19141 ttcgtcatac tgcaacgcta ccgaatagtg ggtcgggtta ttgacaatga catccgcttt
    19201 cggcacatcg gccatcatcc gacgccgtgc ggcagctcgc tgcatctgac ggatccgccc
    19261 tttaacatgg gggtcgcctt cgctttgttt gaactcatca cgaatatcct gccgcgacat
    19321 tcgcagcttt ttcaggtggc tgaagatttg gaaaaagacg tcaaatccca ccattggaat
    19381 gacaccaagc accaccagca gtgcgcatag ccctaccaaa tccatcgcat tacccatggc
    19441 ggtaatcgga gactcggcca ttaagcgcat catatgcggc cagtgatgcc agagaaaaaa
    19501 ccccgtcacg ctgccaacca ggatggtttt caaaattgct ttaagcaact ccgcgccagt
    19561 ctgtgccgag aacatccgtt ttatgcccgg tagcgggttg agtttggaaa acttcggctg
    19621 caaggatttt ccgctaaata ccagcccccc cagcataacc ggagaaatga gcgccaccag
    19681 caccacgccg ctaatcagcg gtagcagcgc cagcatggct tctctgatca gcagaataat
    19741 ttgcccgagg atcagattcg ggtctttgat aatactgtga tcaaaatgca gcccagcgga
    19801 gagcatgccc gacaatcgac gggccagcga cacaccgcca aaccagataa cactaacgcc
    19861 caccagcaaa atcagcagtg aggtcagttc acgggaacgc gggatttgcc cctcttcccg
    19921 cgctttttct agtcggtgag gtgtgggggc ttctgttttg tcgtcgctct cgtcagacac
    19981 gtcgccaatc ctggaaagag tattaagcgt aaatgatgcc agagcgcaaa gcgttcaatg
    20041 gtttgagtaa ggggcaaaac aggcgggatt tagggctttt gctgccacac atcaagcata
    20101 gtgtgcgttt gtcggatgcg gcgtcatcgc cttatcagac cgcctgatat gacgtggtca
    20161 cgccacatca ggcaatacaa atcaaaatcc aagactatcc aacaaatcgt ccacctgatc
    20221 ctgactggct accacaccgg ctttgctggt atcgacctga ggtccattaa gcaaactctg
    20281 gttttcacgt tttggacgcg actcctgttc cgggatgttt tccaacagca ccatcagcaa
    20341 ctggcgttcg atctcctgaa tgacatccat catccgctta atgacctgcc cggtgagatc
    20401 ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg ttagtaaagc tggtatgcgc
    20461 gggtacatct gccagaaatt tccgcgtatc tgttaccagt tcacgggcgt cggcaaggtc
    20521 aatcggatcg gcaaaccagt catcccaacg ttgggttaac gcttttgctg atttctccat
    20581 ttgatcctga tgcggttgtg acgcctcaac actgttcagc gcccgctccg cagcctgggc
    20641 ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg gggatggctt ccgccgcttc
    20701 ggcaatggcc tgatccagtc ccagttcccg caaactgtcg cgcagcatac gcgtcaggct
    20761 gccgatgcgc gcaatgatat cgccagctga atgctcgtca gcaggtttga ttgatggttg
    20821 catcatagtc gcatcctcac atgcccagtt tctcaaagat tttgttgagt ttttcctcca
    20881 gcgtcgcggc ggtaaatggc ttcaccacat agccactggc ccccgcttgc gccgcagcaa
    20941 tgatgttctc tttcttcgct tctgcagtca ccattaacac tggcaatgcc gacatcgcgc
    21001 catccgcacg aatcgttttc agcaattcca ggccatccat gttgggcatg ttccagtcgg
    21061 agataacaaa tccataaccg cctgcctgca acttattgag agcgtcgacg ccatcttccg
    21121 cttcctcaac attattgaat cccagctctt tcagcaggtt acgcactatg cgtcgcatgg
    21181 tggaaaagtc atccacaacc aaaaatttaa gttctttatc cgccatttca cactcctgat
    21241 ttaaatacgt atcgcctgtc cggcactaat ttttgccaac atttgctggc ttacctggtt
    21301 aagatcgacc acttcgcaga caccacccat attgatggcc tcgcgcggca tgccgaacac
    21361 cacgcaactt gcttcgtttt gcgcaagggt ccatgcccca gcctgacgca tcgccaacat
    21421 tcccgccgcg ccgtcgttgc ccataccggt caggatcacc ccaaccgcat tacgccccgc
    21481 ctgtttggcg acagaatgga acaacacatc taccgaaggc cgatgacggt taaccgccgg
    21541 gccatcgtga attttgattt ggtaatttgc gccactacgc gccagctcca tatgccgatc
    21601 gcccggcgca atataggcat gccccggcaa aacacgttcc ccgtcttcgg cttctttaac
    21661 cccgatctgg caaagcttat taagtctgtc ggcaaaagag cgggtgaaac cgggcggcat
    21721 atgctgggta attaacagtg ccgggctgga aagcggcaac ggttgcagta cgtgacgaat
    21781 tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt ttttcagaac tcaacaacgg
    21841 ccctgccttc agcgttgtcg gtgccgacaa tggcttatgt gctgcaaggc tcgcctttgc
    21901 tgctgtacgc accttttcag caatcatttc gctgtacgcc agcattcctt cgcgaatacc
    21961 cagttgcggt ttggtgacaa aatctattgc ccccagctcc agcgcgcgca gcgtgacttc
    22021 tgatcctttg ccggtcaggg aagaaaccat cacaacgggc attggacgca aacgcattaa
    22081 tttttcgagg aaatccagtc cgtccatccg cggcatttca acatccagcg tcagcacatc
    22141 gggattgaat ttcttaatca agtcacgcgc gaccagcgga tcaggcgcgg tcgccaccat
    22201 ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc tggcgcatca gtgccgaatc
    22261 atcgacagat aacaccctga ttttgctcat cgttaatcct tacttagcgc atacaccgtt
    22321 tgaccacgca gcgtgaagcg gcgctcgagg tggctaaagt tttcagagtg acccgcaaac
    22381 agcaatccgt cgggtttaag gagcggaaca aagcggcgca aaatctcctg ctgggtagtt
    22441 tgatcgaagt agatcatgac gttacgacag aagatcgcat caaacggccc cggcacggtg
    22501 tactgtttcg ccagtagatt cagcgaggca aaatcaacat agttcgccag ctcctgacgc
    22561 acgcgtacca gcccttcatg cggtcccgtc cctcgcatga aataccgttg cagttgctgc
    22621 ggcgtcaggt ttttcaactc ttcatggcga tagataccgc ttctggcttt ttccagcact
    22681 tcggtgtcga tatcactggc aaacactttc cagcgtccgg gcgcggtgcc caatgtgtca
    22741 gccagcgtca tcgcaatgct gtacggctct tcgccggtcg aagccgccgc gctccatacg
    22801 cgatactcgc cagaacggcg acgtgcgtga tccgcgagca gagggaaatg atgtgcctca
    22861 cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa acgcctgcca ctcaccgctg
    22921 tgctgattag attccagcaa gttcagataa tgaccgaaat ccgtcagtcc cagcgaacgc
    22981 aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat ggtcagccag aacgatcccg
    23041 gctcgttgat agatcaattg acttatccgc cgaaaatgcg cgtcggaaag cgccaggcgc
    23101 tcggtcatct gtaacaataa agacgtttgc ccacagggca gagatgaagt catagcgcct
    23161 tctcaatcac ttcaggatac cactggcgca atttgtaact gcgccgactc atgtcgtgct
    23221 acttcatgtt cttcaagggt aaataccgcc acacgcgacg aaagatggtc ggcctgattc
    23281 gccagttgtt ccgttgccac cgccgcttct tctaccagcg aggcgttctg ctgtgtcacc
    23341 tgatccatct ggctgacagc ctgcgcaacc tgctcaatcc cccgccgttg ttcttccgag
    23401 gccgaagcaa tttctcccat aatgtcgttc acgcgggtca ccgaactgac aatatcggtc
    23461 atggtcgcgg cggcgttatt caccagtttc gaaccctgct ggacacgatt gactgactct
    23521 tcgatcagcc ctttgatctc ttttgccgcc tgggcgcttc ggctggcaag attgcgcact
    23581 tctcctgcca ctaccgcaaa accacgcccc tgctctccgg cgcgagccgc ttctactgcc
    23641 gcattcaggg ccagaatatt ggtctggaaa gcaattccgt cgataacgct gataatgtcg
    23701 ccaatttttt gcgagctggt ggcgatctcc tgcatggtgt gagtcatggt actgacctga
    23761 acaccgcccg cctgcgccgt tgtcgcggca ttttttgcaa gttccgacgc ctgtcgtgcg
    23821 ttatcggcgt tttgccctac cgtggcggtt aattgctcca tactggcggc cgtttgtgcc
    23881 agcgatgccg cctgctgttc ggtacgactt gagagatcgt tattgcctgc gacaatctcc
    23941 gcgataccaa tgtgcatctc ctggcttccc ttacgcacat cacttaccgt cccacgcaaa
    24001 gcctgctgca tggtcttcag gctggcaaaa atggcggtga tctcattacg accatatacc
    24061 gcaatcggac gcgccagatt acccgcagca atgctgtcaa aatggctacc gataatggcc
    24121 agtggttgaa caatcatctt gcgcgtccac cacagcgcac tgctgatgta gatcgccgca
    24181 acaataatca tgctgataaa caccagcgcc gaaatctgat agttacgctg gctttgcgca
    24241 ctggcggctt ccagcacatg gatgatctcc agctgccagg cctcaaagtt aacgtcaaac
    24301 gcattctgtg atccctgcac cggcgcagtg aggaaatccg aaagttgatt actttcaagc
    24361 caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa aacttttctc cgtctctttt
    24421 tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca taaaactttt aaacagcgtg
    24481 gtggattgcg tcagactggc acgcgccgtc gtcatcaacg ttttaatgtc atccgccgga
    24541 tagctaagcg ctgtcagagt gcctgctttg ttcagcgtgg tactggcctg taacattacc
    24601 gcccgagttt gcgctaatgc cgcacgttgc tgattgctct gctcgacctg attcagtcgt
    24661 tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac tgccaatctg caagatcccg
    24721 cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa tacgattaaa catcaacgct
    24781 ccccatcagg cggcaatgac cgcgttagta aatactcgtc aaaatgtttc ccagtttgga
    24841 tcttgttcag caattcgcag tcgtggctgt gccggtggtt gctcactggc aggacgggat
    24901 ggtgtttgcg gtttattggt gagtgggctg gctgccagac gaaacgcgga aaccgcttgc
    24961 gttaaacgac tagcctgttc ttccagcgca gctgcagcgg cagctgattc ctgcaccagc
    25021 gatgcgttct gttgcgtgac gtgatccatt tccgaaaccg ccaatgcgac ttgatcaatg
    25081 ccacggctct gttcatccga tgccgatgca atctcgccca taatgtcagt cacgcgagtg
    25141 acagcatcga cgatattgtt cattgtttcc ccggcgcttt cgaccagcac cgaaccggta
    25201 tcaacgcgcg agacggagtc ttcaatgaga gctttgatct cttttgccgc ctgggcgcta
    25261 cgactggcaa gattacgcac ttcgcccgcc accacggcaa aaccacggcc ctgttcaccc
    25321 gctcgcgcgg cttcaacagc ggcattcagc gcgaggatat tagtctggaa ggcaataccg
    25381 tcgattacgc tgataatgtc ggcgattttc ttcgaactat cggcgatctc atgcatcgtt
    25441 ttcactacgc catccaccac tttgccgccg tgctgggcgg tgtcggaggc actttgcgcc
    25501 agttgcgagg cctggcgggc gttatcggcg ttttgcttca ctgtcgcggt gagctgctcc
    25561 atgctggcgg cagtttcttc cagcgcggat gcctgctgtt ccgtacggga ggaaagatcg
    25621 gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg catctgaacc ttcgcggaca
    25681 tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa cgctctgcgc caggtcgccc
    25741 atttcactgc gcccgtcaat ggtcagggta ttcgccaggt taccaccggc gatttcgcga
    25801 atgtgagcaa taatttttgc cagcggtgta agcaacatac ggcgaatgcc ataccacgcc
    25861 accagcagaa tcaataccac caccagcgcg ataaccgcca gttgccactg ggcaaatcgg
    25921 taatcatctg cgttgtcagt gacgatatcg cgatacagtt tttcactgct gagggcgtac
    25981 tgagcaaacg cttcgcccat tgcattttgc attccctggg ttggctgagc gaaataagct
    26041 ccagtattgc cataatcaag ataatcaatc agttcagtta acgctgtgta atagttttta
    26101 tatttttcat caatattacg actggtagcg accatttcag gtaacggtgc catgcttttg
    26161 aattttttat aatgagtcgc ggcctgggcc aatgttttcc tggcgctatc gagcaattca
    26221 actttggcat tactttgttg attggaggaa tccatcatca ttcgcaccgc tgaacgactc
    26281 aggttaatgc gcgtttgcag cattaaatcc caggttgacg tcagctcgcc ctgctgttct
    26341 cgtaattgat tggaaaccac aaagctcttc tggctatggt gaagggaaga aaaaaacaga
    26401 ctgccggaaa taagctgtaa cagtgcgaat acccccagca ccattaccaa cagcgtgact
    26461 acgcggatac ggttaatcat aaggcacctt cctgaaaaca agttgatctc gttatcggca
    26521 aggaaggggg aaactttatt gctgatgcca cccgccgcga aattgaaata aaacccgatg
    26581 cgcagatcat cgggttcatt tcaattgagg aaatcaggag aattacgcca cttctgacgc
    26641 cgcgctatct aacagcgcca tctcttcgct gttcagcagt ttttcgatgt tcaccagaat
    26701 caacatacgg tcgcccagtg cgcccagtcc agtgagatat tctgttgaaa gcgtcacggc
    26761 aaactctggt gccggacgaa tttgctccgc cgtcaatgaa agcacgtctg aaacgccgtc
    26821 aaccacgatg ccgaccaccc gctgtccgag attcaggacg ataactaccg tgttgtcgtt
    26881 atagtccaca tccacctggc tgaacttaat tcgtaagtca acaatcggca caataacgcc
    26941 gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc
    27001 gtagccacgg atctcctgca ctttcaggat atcaataccg tactcttcat caccaagggt
    27061 aaataccaga aattcctggc ctgacggctc gctggccagc tttgttacat tcgtcatacc
    27121 ggtcatattg ttaccttttt actcattcag gcggcggtgt tcgccatacg ttgttcgcgg
    27181 tttatcgcct gcaaggcgga gacatcaaca atcagtgcca cgctgccgtc gccaagaatg
    27241 gtcgcagcag aaatgccggg gactttgcga tagttacttt caaggttttt aactacaacc
    27301 tgatgttgac caattaattg atccaccagc aaggcgtagc ggcgaccgcc actttgtaag
    27361 atcaccacaa ttccctgggt agcttcggtt ttcgcgcccg cgacgttgaa cactttccac
    27421 agttcgacga tgggcagata ttcaccccgc acttccagca cccgctcgcc gccggccagt
    27481 ggatgcagat cggcttcacg gggttgcagt gattccataa cagcattcag cggcagaatg
    27541 aatacttcat ccgcaacgcg tacggacatg ccgtcgagga tggccagcgt cagcggcagt
    27601 aaaatgcgga tcgtagtgcc agtaccctgc ttcgactgga tttcaacatg accgcccatc
    27661 tcctggatat tacgtttaac gacgtccatg ccgacgccgc gcccggagac gtcggtgacc
    27721 tgctctgccg tggagaagcc tggtgcaaat atcagcatcg cgacttcgtc gtcgctcatg
    27781 ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca gaattcgctc acggtttagc
    27841 cccgccccat cgtcggtcac ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga
    27901 attaaatttc cgacgctgtt tttacctgcg gcgagccgtt tttctggcag ttcaataccg
    27961 tgatcgaggc tattgcgtac caggtgggtc agcgggtcga taatgcgttc tatcaggctc
    28021 ttgtcgagct cggtggaact gcccaccagc gtcagttcta cctgcttgcc gagttttccc
    28081 gccagatcac gcaccagccg gggatagcga ctaaagacat attccatcgg catcatgcga
    28141 atcgacatca ctgattcctg caaatcacgg gcgttacgtt gtaactgccc catgctggta
    28201 atcaaatcac catgattaac cgggtccagt tcgctggaac gctgggcaag catggactgg
    28261 gtgataacca gctcgccgac gaggttaatt aattgatcaa ccttttctac cgctacacgg
    28321 atgctggtgg attcactgct acgcgtcgtt ttttcccgct ccacgcgacc ggttggcgct
    28381 tgttcggctg ccagtttaag cactggtggg gtggatattt ttggcgagac ttctactgtt
    28441 tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg
    28501 gcgatatcgc ccggtaatat tgccgagaga gaatccgccc ctttcaccac gtcagttaac
    28561 gttgtcagat gccccagctc ttcttccagc aggtcgactt cgctggcctt caggcgcgaa
    28621 aggataattc gtcgcggcaa ctgactgcga ctctgctcat cttgcggttc acttttggca
    28681 accacactta atcgggtcac tgcggatggc gtttcgcctt tcgcttctaa tgccagttga
    28741 cgcaaggcct ggcagatata atcgaagctg gcggcatccg gctcttgcga ctgtttataa
    28801 gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa acagattgat aatgtcggtg
    28861 ttgagttgca tctcacctcg tctggcttca tcgagcaggt tttccatcag atgcgtggtt
    28921 tcctgcaaaa cgctgaagcc aaaagttcct gcccctcctt tgatcgagtg ggcagcccga
    28981 aagatggcat tcaattgttc ggcatctggc gcttccggct gcaaaaccag cagatgctgc
    29041 tccatatcag ccaacagttc gtccgcttca tcaaaaaatg tctgataaaa atcgcttata
    29101 tccatgctca cgctgtcacc tcggttcggc tgatggcatt gtgggaacac tgacctgtgg
    29161 tgcaacctca ggtttttcca gggcgcttac tggctcattc tggctttcgg cgttttcatg
    29221 caaaatggcc tgttcggctt gtttgttcag taccagcagg ctgatgcgac ggttgacggc
    29281 atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc atgccgacca cacgtaacac
    29341 tttgccgcta tccaaccctc cgaccatcag ttcgcggcgg gatgcattgg cccgatcggc
    29401 agaaagctcc cagttgctgt aacctttctc accactggcg taggggaaat catcggtatg
    29461 acccgaaagg ctaatacggt tgggaatacc attcagaacg ggcgcgatgg cacgcagaat
    29521 gtcgcgcata tagggttcga cgtcggcact gccagttcta aacatcgggc gattctggct
    29581 atcgatgatc tgaatacgta gaccttcctg gaccagatcg attttgagat gtggacgtaa
    29641 cgcccgcagt ttcggatcgg actctatcaa ttggtcgaga tcgccacgca atttccgcaa
    29701 tcgactttgc tccatgcgtt ttttcaactc ttcgatgttc ggctgcttat tcacttcccc
    29761 ctggctttgg gtgtaatcat cgccaccgcc gggaattggg ctttcactat tagaaatgcg
    29821 atcgccgccc gtaaccgcag tcgccagtgg agtccggaag tactccgcaa tctgaatcaa
    29881 ctcttttggg ctggagatgg agatcagcca catcaccaga aaaaaggcca tcatcgcggt
    29941 cataaagtcg gcataagcaa tcttccacga tccatgtgct gccccgtggc ttttggcttt
    30001 gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc
    30061 tgttgttgcg gatttttcac cgcacgcaca tgctcttcca gttcaataaa cgacggacgt
    30121 tcgctggaat agagcgtttt gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc
    30181 agattagaaa gcagtgtgac tttgacgcac tgcatcattt tgctggtttc ggcgcttttc
    30241 tgacgtaaaa cactcgctaa tggggaaata aatccgtaag ccaataaaat gccgaggaaa
    30301 gtccccacca tcgcatgtgc gataagcgcc cccagctcgg cggcaggacg atcggctgaa
    30361 cctaacgcgt gaacgacccc cattacagcc gcgacaatac caaacgccgg aagtgagtcc
    30421 ccgaccagcg ccagactgtt cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct
    30481 tcatccatca gggcttcgat ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga
    30541 taatcgacga taaaatcaag catgacgcta tccgcgatga tgcgtgggta gctggcgaag
    30601 atctcgctct cacggggatt ttcaatatca cgttccagcg aaaacatccc catctgccgc
    30661 gatttcgcca tcaaccgata aagcagagcc agcagatcca tatacattgc tttggtgtat
    30721 ttggagcgac gaaacagcaa cggcagcgct ttcagcgtgc ctttaatcgc ttttccatta
    30781 ttgccgacaa taaacgaccc aatccctgca ccggcaataa tcaccagttc agcgggttga
    30841 tagagtgctc caaggcttcc accggtcatc aaataaccgc cgaaaactgt accgagaaca
    30901 accaggtaac ctaataagat aagcacgaca tcatccttcc actgttgacc atgacaggat
    30961 gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac
    31021 cgctgctgga atgttgcgcc acaccgtatc agttaaacag cctgtactct ctgttcatcc
    31081 agcagttgtg ggataatatc ggcaggattc tgggaaagtt tacgtctttt tactgcccgg
    31141 gatggcggtt gacataagct gcaggcaaag ctgccaacag gctggtgagc gtgggtaata
    31201 aaattgccgc cgcagcagtt gcagctggaa agttgcagta atccactttc aacaaaccgc
    31261 accaatgtcc aggcacgggt taatgccagc agcggtcctt cttctgcttg cgggcactgt
    31321 tcaaggtata aacggtaggc tttgatcacc gcatcgacgc cattacacaa accggttttc
    31381 agtaaaaact gccatgcatt acagaacatc gaagcatgaa cgttttgttc ccaagtcata
    31441 aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg ggcttccgcg cagttcttta
    31501 taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga
    31561 gcgcccaggg tgatcaattc cattgccagc tgaatatccc gcgcttcctg aacaatgctt
    31621 ttttcactca tgatcaggcc cttttcttgc gcagcgcttc ttcaggctga ttaacatcat
    31681 tcagcaagcg tgttgagagc atgatgccgg tatgaatttg ctggagatcg tcaacgcggg
    31741 aatcttgcgt caacagagta atcgtctggt ggctgtcaaa acggaagtga caaaccagct
    31801 gattggtttc tgccagctta accatttgcg gaagagtcag tgccgctaat gttgtcgcca
    31861 tttcttcatt tatgccgaga cgaaacatag cggacgcttt gtcctgaaca atcagacgct
    31921 gtgcaagtag taaatatgac aagttgatgt cataaatgtg tttcagcaac tcggaggtat
    31981 gcattattcc cacccagaat aaccaacttt atttttatgc ggtttcaccg caccccgtga
    32041 tgtcgccggg aagccccggt aaaaaataat tagcattaga atagttgcga taagctgcaa
    32101 taagcagagc cacctttttg gtttaataag tccttacaaa tagaaggggg tctttacact
    32161 tatctaagat ttttcctaaa tcgacgcaac tgtactcgtc actacacgca catacaacgg
    32221 aggggggctg cgattttcaa taatgtgtga tgcagatcac acaaaacact caattactta
    32281 acataaatgt ataagtcata cttttgtttt gggtgtattt caatctgtta aaaagttttt
    32341 cgctacgcta gcaagcaaaa atgaaacagg aataatcgaa atgggatgtt gcgcacagtt
    32401 aaaataactc accgtaaata atcatctgct ataaataatc actttcatgc aataccagat
    32461 aagctatttt taaacag
//
LOCUS       DABSYT020000047        32205 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.047, whole genome shotgun sequence.
ACCESSION   DABSYT020000047 DABSYT020000000
VERSION     DABSYT020000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.047"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            42..341
                     /locus_tag="ID725_RS13020"
                     /old_locus_tag="pgaptmp_002604"
     CDS             42..341
                     /locus_tag="ID725_RS13020"
                     /old_locus_tag="pgaptmp_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851181.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(388..1275)
                     /gene="rarD"
                     /locus_tag="ID725_RS13025"
                     /old_locus_tag="pgaptmp_002605"
     CDS             complement(388..1275)
                     /gene="rarD"
                     /locus_tag="ID725_RS13025"
                     /old_locus_tag="pgaptmp_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGV7851182.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPTDEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1327..1794)
                     /gene="yigI"
                     /locus_tag="ID725_RS13030"
                     /old_locus_tag="pgaptmp_002606"
     CDS             complement(1327..1794)
                     /gene="yigI"
                     /locus_tag="ID725_RS13030"
                     /old_locus_tag="pgaptmp_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGV7851183.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1959..2828
                     /gene="pldA"
                     /locus_tag="ID725_RS13035"
                     /old_locus_tag="pgaptmp_002607"
     CDS             1959..2828
                     /gene="pldA"
                     /locus_tag="ID725_RS13035"
                     /old_locus_tag="pgaptmp_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGV7851184.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2961..4790
                     /gene="recQ"
                     /locus_tag="ID725_RS13040"
                     /old_locus_tag="pgaptmp_002608"
     CDS             2961..4790
                     /gene="recQ"
                     /locus_tag="ID725_RS13040"
                     /old_locus_tag="pgaptmp_002608"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGV7851185.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4854..5474
                     /gene="rhtC"
                     /locus_tag="ID725_RS13045"
                     /old_locus_tag="pgaptmp_002609"
     CDS             4854..5474
                     /gene="rhtC"
                     /locus_tag="ID725_RS13045"
                     /old_locus_tag="pgaptmp_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGV7851186.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5536..6156)
                     /gene="rhtB"
                     /locus_tag="ID725_RS13050"
                     /old_locus_tag="pgaptmp_002610"
     CDS             complement(5536..6156)
                     /gene="rhtB"
                     /locus_tag="ID725_RS13050"
                     /old_locus_tag="pgaptmp_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGV7851187.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6267..7289
                     /gene="pldB"
                     /locus_tag="ID725_RS13055"
                     /old_locus_tag="pgaptmp_002611"
     CDS             6267..7289
                     /gene="pldB"
                     /locus_tag="ID725_RS13055"
                     /old_locus_tag="pgaptmp_002611"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGV7851188.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7297..8097
                     /gene="yigL"
                     /locus_tag="ID725_RS13060"
                     /old_locus_tag="pgaptmp_002612"
     CDS             7297..8097
                     /gene="yigL"
                     /locus_tag="ID725_RS13060"
                     /old_locus_tag="pgaptmp_002612"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGV7851189.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8173..9072
                     /gene="bioP"
                     /locus_tag="ID725_RS13065"
                     /old_locus_tag="pgaptmp_002613"
     CDS             8173..9072
                     /gene="bioP"
                     /locus_tag="ID725_RS13065"
                     /old_locus_tag="pgaptmp_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGV7851190.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8960..9913)
                     /gene="metR"
                     /locus_tag="ID725_RS13070"
                     /old_locus_tag="pgaptmp_002614"
     CDS             complement(8960..9913)
                     /gene="metR"
                     /locus_tag="ID725_RS13070"
                     /old_locus_tag="pgaptmp_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGV7851191.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10149..12410
                     /gene="metE"
                     /locus_tag="ID725_RS13075"
                     /old_locus_tag="pgaptmp_002615"
     CDS             10149..12410
                     /gene="metE"
                     /locus_tag="ID725_RS13075"
                     /old_locus_tag="pgaptmp_002615"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGV7851192.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12450..13265)
                     /gene="ysgA"
                     /locus_tag="ID725_RS13080"
                     /old_locus_tag="pgaptmp_002616"
     CDS             complement(12450..13265)
                     /gene="ysgA"
                     /locus_tag="ID725_RS13080"
                     /old_locus_tag="pgaptmp_002616"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGV7851193.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13527..14288
                     /gene="udp"
                     /locus_tag="ID725_RS13085"
                     /old_locus_tag="pgaptmp_002617"
     CDS             13527..14288
                     /gene="udp"
                     /locus_tag="ID725_RS13085"
                     /old_locus_tag="pgaptmp_002617"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGV7851194.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14429..15856
                     /gene="rmuC"
                     /locus_tag="ID725_RS13090"
                     /old_locus_tag="pgaptmp_002618"
     CDS             14429..15856
                     /gene="rmuC"
                     /locus_tag="ID725_RS13090"
                     /old_locus_tag="pgaptmp_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGV7851195.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15951..16706
                     /gene="ubiE"
                     /locus_tag="ID725_RS13095"
                     /old_locus_tag="pgaptmp_002619"
     CDS             15951..16706
                     /gene="ubiE"
                     /locus_tag="ID725_RS13095"
                     /old_locus_tag="pgaptmp_002619"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGV7851196.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16720..17325
                     /gene="ubiJ"
                     /locus_tag="ID725_RS13100"
                     /old_locus_tag="pgaptmp_002620"
     CDS             16720..17325
                     /gene="ubiJ"
                     /locus_tag="ID725_RS13100"
                     /old_locus_tag="pgaptmp_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGV7851197.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17322..18962
                     /gene="ubiB"
                     /locus_tag="ID725_RS13105"
                     /old_locus_tag="pgaptmp_002621"
     CDS             17322..18962
                     /gene="ubiB"
                     /locus_tag="ID725_RS13105"
                     /old_locus_tag="pgaptmp_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGV7851198.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19041..19310
                     /gene="tatA"
                     /locus_tag="ID725_RS13110"
                     /old_locus_tag="pgaptmp_002622"
     CDS             19041..19310
                     /gene="tatA"
                     /locus_tag="ID725_RS13110"
                     /old_locus_tag="pgaptmp_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGV7851199.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19314..19829
                     /gene="tatB"
                     /locus_tag="ID725_RS13115"
                     /old_locus_tag="pgaptmp_002623"
     CDS             19314..19829
                     /gene="tatB"
                     /locus_tag="ID725_RS13115"
                     /old_locus_tag="pgaptmp_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGV7851200.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19832..20608
                     /gene="tatC"
                     /locus_tag="ID725_RS13120"
                     /old_locus_tag="pgaptmp_002624"
     CDS             19832..20608
                     /gene="tatC"
                     /locus_tag="ID725_RS13120"
                     /old_locus_tag="pgaptmp_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGV7851201.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20650..21432
                     /gene="tatD"
                     /locus_tag="ID725_RS13125"
                     /old_locus_tag="pgaptmp_002625"
     CDS             20650..21432
                     /gene="tatD"
                     /locus_tag="ID725_RS13125"
                     /old_locus_tag="pgaptmp_002625"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGV7851202.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21429..21917)
                     /gene="rfaH"
                     /locus_tag="ID725_RS13130"
                     /old_locus_tag="pgaptmp_002626"
     CDS             complement(21429..21917)
                     /gene="rfaH"
                     /locus_tag="ID725_RS13130"
                     /old_locus_tag="pgaptmp_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGV7851203.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22084..23577
                     /gene="ubiD"
                     /locus_tag="ID725_RS13135"
                     /old_locus_tag="pgaptmp_002627"
     CDS             22084..23577
                     /gene="ubiD"
                     /locus_tag="ID725_RS13135"
                     /old_locus_tag="pgaptmp_002627"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGV7851204.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23623..24324
                     /gene="fre"
                     /locus_tag="ID725_RS13140"
                     /old_locus_tag="pgaptmp_002628"
     CDS             23623..24324
                     /gene="fre"
                     /locus_tag="ID725_RS13140"
                     /old_locus_tag="pgaptmp_002628"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGV7851205.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24516..25679)
                     /gene="fadA"
                     /locus_tag="ID725_RS13145"
                     /old_locus_tag="pgaptmp_002629"
     CDS             complement(24516..25679)
                     /gene="fadA"
                     /locus_tag="ID725_RS13145"
                     /old_locus_tag="pgaptmp_002629"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGV7851206.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25689..27878)
                     /gene="fadB"
                     /locus_tag="ID725_RS13150"
                     /old_locus_tag="pgaptmp_002630"
     CDS             complement(25689..27878)
                     /gene="fadB"
                     /locus_tag="ID725_RS13150"
                     /old_locus_tag="pgaptmp_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGV7851207.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28068..29399
                     /gene="pepQ"
                     /locus_tag="ID725_RS13155"
                     /old_locus_tag="pgaptmp_002631"
     CDS             28068..29399
                     /gene="pepQ"
                     /locus_tag="ID725_RS13155"
                     /old_locus_tag="pgaptmp_002631"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGV7851208.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29399..30013
                     /gene="yigZ"
                     /locus_tag="ID725_RS13160"
                     /old_locus_tag="pgaptmp_002632"
     CDS             29399..30013
                     /gene="yigZ"
                     /locus_tag="ID725_RS13160"
                     /old_locus_tag="pgaptmp_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGV7851209.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30052..31503
                     /gene="trkH"
                     /locus_tag="ID725_RS13165"
                     /old_locus_tag="pgaptmp_002633"
     CDS             30052..31503
                     /gene="trkH"
                     /locus_tag="ID725_RS13165"
                     /old_locus_tag="pgaptmp_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGV7851210.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31515..32060
                     /gene="hemG"
                     /locus_tag="ID725_RS13170"
                     /old_locus_tag="pgaptmp_002634"
     CDS             31515..32060
                     /gene="hemG"
                     /locus_tag="ID725_RS13170"
                     /old_locus_tag="pgaptmp_002634"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGV7851211.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7490 a         8148 c         8792 g         7775 t
ORIGIN      
        1 tttttattct ataaacaata tcttgggtga atggattcta tatgggaaat ggtaactttt
       61 ggcagttgtt cttgggtaaa gaaccttaca gcaaaaaatc tgattttgtt aaggcatggt
      121 tattgtggtg ggctattacc atccttgttc tatttatttt aagcctgact acgacattaa
      181 atttaagaag tataggtata gggttaagtt ttgggttttg tcaaatcctt ggtagatata
      241 cgatgtctct aggatggagt aagtactggg gagttttggg gggatttcct gtaacaaata
      301 ttgttctttt tcttatctta atttttttca aaaaaagata aataagtcag tgggtaggct
      361 ctttaaacgg cctacccatg ttaattctta actttttcta cgctgcgtat aaatcgcatc
      421 catcacaaaa attgccagcg ccacccaaat aaaggtgaaa gtcaccatct tatcggcacc
      481 cggtttttca ccataaaacg tcacagccag caggaacatc agcgtcgggc caatgtactg
      541 gaaaaagcct aacgttgaga gacgaaggcg cgtggcagcg gcggtaaaac acaacagcgg
      601 tacggtagtg acaataccgg cggcgatcag cagtaaattc agcgacatcg ggttttgccc
      661 catatggctg gttgagctgt cggcaatagc aaacaggtaa atcgccgcca cgggcagcag
      721 ccacatggtt tcaattaaca tgccggtttg tgcttctacc gcaatcttct tgcgcaccag
      781 gccgtagaag gcaaaactaa atgccagccc cagcgcgata ataggtagcg aaccaaaagt
      841 ccacagctgg actaacacgc cacatatcgc cagaatcacc gccagccatt gcatccggcg
      901 gaatcgctcg ccgaggaaaa tcatccccag cacaatgttc accagcgggt taataaagta
      961 accaaggctc gcttccagca tatggtgatt gttcaccgcc cagataaaca gtagccagtt
     1021 gccaccaatc agcacggcag agactgccag cataaaaatt ttctgtggcg tctgaatcag
     1081 cgtttttaaa taggaccact ggcggcaaat gctcatcagc accaccataa agaaaaacga
     1141 ccagatcacg cgatgcgtca ggatctcatc ggtgggcacg tagtaaatca acttgaagta
     1201 cgctggggct ataccccaaa taaaataagc ggcaagagcg agtaatacgc cctgccgcgt
     1261 ttgttttgca tccatcggga atactcattt ttaattggta acagcagttt acctgctttt
     1321 atgtcttcaa cctaccatat aggtggcggt ggcactggca atataaagct gttcttcatt
     1381 gtgtaattca acgcgggcga cggcgacttt attgcctgca cgcaacaggc tactggtagc
     1441 agtaaaacgc tcgcctctgc ctgggcgcag ataatcgacg cgaagatcaa tggtccccat
     1501 ccgcgatagc cgctggcgta gttcatcttc actgatggtt tcgtggcggg ttaaggtact
     1561 tcctacgcac accagaccgg cggcgacatc cagcgccgac gcaatgaccc cgccgtgcaa
     1621 aatgctttgc gcccagttgc ccaccatcat tggctgattt ttaaaggcca gctgtgcgaa
     1681 ctctttttcg taacgctcca gttccatccc caatgcgcgg ttaaatggca tgtgataaac
     1741 aaacatctca cccactaatt tcagggcttg ttcagcggtc agtacggcag acatatcatc
     1801 cttacacttc attggttaat gaaatgttga ttttatgatt ctttgttgtt ggtttctact
     1861 ttaggaaggg ataactaacg gcaatggagt taagtatgta aaataccccg cagaaaaaca
     1921 ttcaccttat caataattcg ttacggagaa cacgaccgat gcggactctg cagggctggt
     1981 tgttgccggt gtttatgttg cctatggcag tatatgcaca agaggcaacg gtgaaagagg
     2041 tgcatgacgc gccagcggtg cgtggcagta ttatcgccaa tatgctgcag gagcatgaca
     2101 atccgttcac gctctatcct tatgacacca actacctcat ctacacccaa accagcgatc
     2161 tgaacaaaga agcgattgcc agttacgact gggcggaaaa tgcgcgtaag gatgaagtaa
     2221 agtttcagtt gagcctggca tttccgctgt ggcgtgggat tttaggcccg aactcggtgt
     2281 tgggtgcgtc ttatacgcaa aaatcctggt ggcaactgtc caatagcgaa gagtcttcac
     2341 cgtttcgtga aaccaactac gaaccgcaat tgttcctcgg ttttgccacc gattaccgtt
     2401 ttgcaggttg gacgctgcgc gatgtggaga tggggtataa ccacgactct aacgggcgtt
     2461 cagacccgac ctcccgcagc tggaaccgcc tttatactcg cctgatggca gaaaacggta
     2521 actggctggt agaagtgaag ccgtggtatg tggtgggtaa tactgacgat aacccggata
     2581 tcaccaaata tatgggttac taccagctta aaatcggcta tcacctcggt gatgcggtgc
     2641 tcagtgcgaa aggacagtac aactggaaca ccggctacgg cggcgcggag ttaggcttaa
     2701 gttacccgat caccaaacat gtgcgccttt atactcaggt ttacagcggc tatggcgaat
     2761 cgctcatcga ctataacttc aaccagaccc gtgtcggtgt gggggttatg ctaaacgatt
     2821 tgttttgatg aacggttgag tggttggcaa atctggaatc cagcatccag gattaccctc
     2881 tcagagacta aaagcattgc agtttctcgc gcaggcgctg aaaatagcgc ctgtttttat
     2941 ttcaggcaat cggggtgaat gtggcgcagg cggaagtgtt gaatctggag tccggagcta
     3001 aacaggtttt acaagaaacc tttggctacc aacagtttcg ccccggccag gaagaaatta
     3061 tcgacactgt gctttccggt cgcgattgcc tggtcgtcat gcccaccggt ggcggaaaat
     3121 ccctttgcta tcaaattcct gccttattgc taaacggcct taccgtggtt gtttcaccgc
     3181 tgatttcgtt gatgaaagat caggtggatc aactgcaagc caacggcgtg gcggcggcgt
     3241 gccttaactc gacgcaaacc cgcgaacagc aacttgaagt gatgacaggc tgccgcaccg
     3301 ggcaaattcg tctgctttat atcgccccgg aacgcctgat gctggataac tttcttgagc
     3361 atctggcgca ctggaatccg gtgttattag ccgttgatga agcgcactgt atctcccaat
     3421 ggggccacga tttccgcccg gaatatgccg cgctcggtca gttgcgccag cggttcccga
     3481 cgctgccgtt tatggcgctg accgccacag ccgacgacac cacgcgccag gatatcgtgc
     3541 gcctgctggg gctgaacgat ccgctgattc aaatcagcag ttttgaccgt ccgaatattc
     3601 gctacatgct gatggagaaa ttcaaaccgc tcgatcagtt gatgcgctac gtgcaggaac
     3661 agcgcggtaa gtccggcatt atctactgca acagccgggc gaaagtagaa gacaccgctg
     3721 cgcgcctgca aagcaagggt attagcgcgg cggcctatca tgccgggctg gaaaataatg
     3781 tccgcgccga cgtgcaggag aaattccagc gcgatgacct gcaaattgtg gtggcgacgg
     3841 tggcgttcgg catgggcatc aataaaccaa acgttcgctt cgtggtccac tttgatattc
     3901 cgcgcaatat cgaatcctat tatcaggaaa ccggtcgcgc cgggcgtgat ggcctgcctg
     3961 cggaagcgat gttgttttac gatccagctg atatggcgtg gctgcgccgt tgtctggaag
     4021 agaagccgca ggggcagttg caggatatcg agcgccacaa actcaatgcg atgggcgcgt
     4081 ttgccgaagc gcaaacttgc cgtcgtctgg tattgctgaa ctattttggc gaagggcgtc
     4141 aggagccgtg cgggaactgc gatatctgcc tcgatccgcc gaaacagtac gacggttcaa
     4201 ccgatgctca gattgccctt tccaccattg gtcgtgtgaa tcagcggttt gggatgggtt
     4261 atgtggtgga agtgattcgt ggtgctaata accagcgtat ccgcgactat ggtcatgaca
     4321 aactgaaagt ctatggcatg ggccgtgata aaagccatga acattgggtg agcgtgatcc
     4381 gccagctgat tcacctcggc ctggtgacgc aaaatattgc ccagcattct gccctacaac
     4441 tgacagaggc cgcgcgcccg gtgctgcgcg gcgaatcctc tttacaactt gccgtgccgc
     4501 gtatcgtggc gctcaaaccg aaagcgatgc agaaatcgtt cggcggcaac tatgatcgca
     4561 aactgttcgc caaattacgc aaactgcgta aatcgattgc cgatgaaagt aatgtcccgc
     4621 cgtacgtggt gtttaacgac gcaaccttga ttgagatggc tgaacagatg ccgatcaccg
     4681 ccagcgaaat gctcagcgtt aacggcgttg ggatgcgcaa gctggaacgc tttggtaaac
     4741 cgtttatggc gctgattcgt gcgcatgtcg atggcgacga cgaagagtag tcagcagcat
     4801 aaaaaagtgc caggatgaag actccgtaaa cgtttccccc gcgagtcaaa tgtatgttga
     4861 tgttatttct caccgtcgcc atggtgcaca ttgtggcgct tatgagtccc ggtcccgatt
     4921 tcttttttgt ctctcagacc gctgtcagtc gttcccgtaa agaagcgatg atgggcgtgc
     4981 tgggtattac ctgcggcgtt atggtttggg cagggattgc gttgcttggc ctgcatttga
     5041 ttatcgaaaa aatggcctgg ctgcatacgc tgattatggt tggcggtggc ctgtatctct
     5101 gctggatggg ttaccagatg ctgcgcggcg cgctgaaaaa agaggcggtt tctgcacctg
     5161 cgccacaggt cgagctggcg aaaagtgggc gcagtttcct gaaaggttta ctgaccaatc
     5221 tcgctaatcc gaaagcgatt atctactttg gctcggtgtt ctcattgttt gtcggtgata
     5281 acgttggcac taccgcgcgc tggggcattt tcgcgttgat tattgtcgaa acgctggcgt
     5341 ggtttactgt ggtggccagc ctgtttgccc tgccgcaaat gcgccgtggt tatcaacgtc
     5401 tggcgaagtg gattgatggt tttgccgggg cgttatttgc cggatttggc attcatttga
     5461 ttatttcgcg gtaatgccag acgcgtcttc agagtaagtc ggataaggcg tttacgccgc
     5521 atccgacatt atttttcacg catgcctcgc cgatgctaac agcgctccca ccagcataaa
     5581 caacgagccg aaaatcttat tcagcgcctt catctgcttt ggtcctttaa tccatagagc
     5641 aatccgttga gcaagggtgg cgtaaccgat catcacaata atatcgacca caatagtggt
     5701 gacaccgagc acgatatact gcatcagttg cggctgttgc ggcatgatga attgcggaaa
     5761 tagcgccgcc agaaacacaa tacttttggg attggtgaga ttcacaaaaa ctgcgcgctg
     5821 gaacaaatgt cgacgcgatt gagtagaggc cagcgattta agatcaattg cgccagcggc
     5881 gcgccattgc tgtattccca gccaaatcaa gtaagccgcg cctgcccact tcaacacttc
     5941 aaacgcaatc actgagcggg aaaatagcgt ccccaacccc acgccaacca gcacaatatg
     6001 aatcgccagt ccggtctgaa gcccggcaat agacgccacc gcgccgcgat aaccgtggtt
     6061 gagcgaggtg gtcatagtgt tgattgcacc agagcctggc gacaggctta aaatgattga
     6121 tgtcagtagg taggcaaacc accattctaa ggtcatgatg aactcccggt gtgtctattt
     6181 ttgtgccaca atacgctact gtcgcagcgt tgtgtcaggc acgctaaaaa aaacgatttt
     6241 acgtggttta agaggcagat tacccgatgt ttcagcagca aaaagactgg gaaacaagag
     6301 aaaacgcgtt tgctgctttt accatgggac cgctgactga tttctggcgt cagcgtgatg
     6361 aagcagagtt tactggtgtg gatgacattc cggtgcgctt tgtccgtttt cgcgcacagc
     6421 accatgaccg ggtggtagtc atctgcccgg ggcgtattga gagttacgta aaatatgcgg
     6481 aactggccta cgatttgttc catttggggt ttgatgtctt aatcatcgac catcgcgggc
     6541 agggacgttc cggtcgcctg ttagccgatc cgcatctcgg gcatgttaat cgctttaatg
     6601 attatgttga tgatctggcg gcattctggc aacaggaggt ccagcccggt ccgtggcgta
     6661 aacgctatat actggcacat tcgatgggcg gtgcgatctc cacattattt ctgcaacgcc
     6721 atccaggtgt atgtgacgcc attgcgctaa ctgcgccaat gtttgggatc gtgattcgta
     6781 tgccgtcatt tatggcacgg cagatcctca actgggccga agcgcatcca cgtttccgtg
     6841 atggctatgc aataggcacc gggcgctggc gcgcgttgcc gtttgctatc aacgtactga
     6901 cccacagcag acagcgatat cgacgtaact tacgcttcta tgctgatgac ccaacgattc
     6961 gcgtcggtgg gccgacctac cattgggtac gcgaaagtat tctggctggc gaacaggtgt
     7021 tagccggtgc gggtgatgac gccacgccaa cgcttctctt gcaggctgaa gaggaacgcg
     7081 tggtggataa ccgcattcat gaccgttttt gtgaactccg caccgccgcg ggccatcctg
     7141 tcgaaggggg acgaccgctg gtaattaaag gtgcgtacca tgagatcctt tttgaaaagg
     7201 acgcaatgcg ctcagttgcg ctccacgcca tcgttgattt tttcaacagg cataactcac
     7261 ccagcggaaa ccgctctaca gaggtttaaa tttcttatgt accaggttgt tgcgtctgat
     7321 ttagatggca cgttactttc tcccgaccat acattatccc cttacgccaa agaaaccctg
     7381 aagctgctca ccgcgcgcgg catcaacttt gtgtttgcga ccggtcgtca ccacgttgat
     7441 gtggggcaaa ttcgcgataa tctggagatt aagtcttaca tgattacctc caatggtgcg
     7501 cgcgttcacg atctggatgg taatctgatt tttgctcata acctggatcg cgacattgcc
     7561 agcgatctgt ttggcgtagt caacgacaat ccggacatca ttactaacgt ttatcgcgac
     7621 gacgaatggt ttatgaatcg ccatcgcccg gaagagatgc gcttttttaa agaagcggtg
     7681 ttcaaatatg cgctgtatga gcctggatta ctggagccgg aaggcgtcag caaagtgttc
     7741 ttcacctgcg attcccatga acaactgctg ccgctggagc aggcgattaa cgctcgttgg
     7801 ggcgatcgcg tcaacgtcag tttctctacc ttaacctgtc tggaagtgat ggcgggcggc
     7861 gtttcaaaag gccatgcgct ggaagcggtg gcgaagaaac tgggctacag cctgaaggat
     7921 tgtattgcgt ttggtgacgg gatgaacgat gccgaaatgc tgtcgatggc ggggaaaggc
     7981 tgcattatgg gcagtgcgca ccagcgtctg aaagaccttc atcccgagct ggaagtgatt
     8041 ggtactaatg ccgacgacgc ggtgccgcat tatctgcgta aactctattt atcgtaatcg
     8101 ttctttattt ggtcagttgt caacctgata cttcgctaca atggatactc gttaatcaaa
     8161 gagttttcca ttgtggcgct acttatcatc accacgattc tgtgggcctt ctcctttagc
     8221 ttttatggcg agtaccttgc ggggcacgtc gatagctatt ttgcggtgct ggtgcgcgtt
     8281 ggcctggcgg cactcgtttt tctgccgttt ctgcgtaccc gtggcaatag cctgaaaaca
     8341 gtcggcctgt atatgctggt gggcgcgatg cagctcggcg tgatgtatat gctgagtttc
     8401 cgcgcttatc tctacctgac ggtttccgaa ctgctgctgt tcaccgtact gacgccgctc
     8461 tacatcacgc tgatttatga catcatgagt aagcgccgtc tgcgctgggg atatgctttt
     8521 agtgccttgt tggcggtgat tggtgccggg attattcgct atgatcaggt caccgaccat
     8581 ttctggactg gcttgttgct ggtgcaactc tccaatatca cttttgccat cggcatggtg
     8641 ggttacaaac gcctgatgga aactcgcccg atgccgcagc ataacgcctt tgcgtggttc
     8701 tatcttggcg cgttcctggt ggcggtaatt gcatggttct tgctgggaaa tgcgcagaaa
     8761 atgccgcaaa ccacgttgca atggggcatt ctggtgtttc ttggcgtggt ggcttccggg
     8821 attggctact ttatgtggaa ctacggcgca acccaagtag acgccggaac gctgggcatt
     8881 atgaataata tgcacgttcc ggcagggctg ctggttaacc tggctatctg gcaccaacag
     8941 ccgcactggc caacgtttat tacaggcgcg ctggtgatcc tggcctcact gtgggtgcat
     9001 cgtaagtggg tcgctccgcg ctcttcacaa acggcagatg atcgcaggcg tgattgcgcg
     9061 ctgagcgaat aaacgcttcc gtaactggct gacgctgctc gccatcgcgc acggcggcgt
     9121 acagtcggct ccacaagcct tcgcccaggg ttttggtcac caccagaccc tggcgctcaa
     9181 aactctctac tacccaatgt ggtagcgcgg caatacccat ccgcgcggca accatctgaa
     9241 tcaacaataa ggtgttatcg acgcttttca gcgacgggct aacgcctgcg ggctggagaa
     9301 aatgccgcca gacatccagt cggctacgct gcaccggata aatcaacagc gtttcgctgg
     9361 cgagatcttc cggcgtgatt cgcgttttcg ccgccagtgg atggtcaggt gctaacacca
     9421 gacgcacttc atagtcgaac atcggcgaat aatgcaggcc actacgcggc agaatatcgg
     9481 aggtcatcac cagatccagc tcgccctgct gcaacgcagg ctgcgggtca aacgttacgc
     9541 ccgatttaaa atccatctcc acctgcggcc agttcttatg gaaattttct aatgcgggcg
     9601 tcagccactg aatacagcta tggcactcaa tggcaatgcg caggcgcgtc tgttgcggtt
     9661 cattgcaggc ttgcagcgcc tggctaattt gcggcagcac ctggtttgcc agttgcaaca
     9721 ggatttcccc ctgtggagta aagcgtagcg gctggctctt acgcacaaat agccggaagc
     9781 caaggcgttg ttccagatcg ctaaactggt gagacagggc ggattgcgtt tgatgcaacg
     9841 tcgccgcagc ggctgcgaga gagccgcagt tccgcaacgc ttgtagcgtt ttcaggtgtt
     9901 ttacttcgat catgaaagtc cttcacttcg gcatgaataa tttgcgcttg aggaatatac
     9961 agtaaccgcc aattatggat gtgtaaacat ctggacggct aaaatccttc gtcttttaaa
    10021 tttatggtgc gttggctgcg tttctccacc ccggtcactt acttcagtaa gctcccgggg
    10081 atgaataaac ttgccgcctt ccctaaattc aaaatccata ggatttacat ataattagag
    10141 gaagaaaaat gacaatattg aatcacaccc tcggtttccc tcgcgttggc ctgcgtcgcg
    10201 agctgaaaaa agcgcaagag agttattggg cggggaactc cacgcgtgaa gaactgctgg
    10261 cggtagggcg tgaattgcgt gctcgtcact gggatcaaca aaagcaagcg ggtatcgacc
    10321 tgctgccggt gggcgatttt gcctggtacg atcatgtact gaccaccagt ctgctgctgg
    10381 gtaacgttcc ggcgcgtcat cagaacaaag atggttcggt agatatcgac accctgttcc
    10441 gtattggtcg tggacgtgcg ccgactggcg aacctgcggc ggcagcggaa atgaccaaat
    10501 ggtttaacac caactatcac tacatggtgc cggagttcgt taaaggccaa cagttcaaac
    10561 tgacctggac gcagctgctg gacgaagtgg acgaggcgct ggcgctgggc cacaaggtga
    10621 aacctgtgct gctggggccg attacctggc tgtggctggg gaaagtgaaa ggtgaacagt
    10681 ttgaccgcct gagcctgctg aacgacattc tgccggttta tcagcaagtg ctggcagaac
    10741 tggcgaaacg tggcatcgag tgggtacaga ttgatgaacc cgcgctggta ctggaactac
    10801 cacaggcgtg gctggacgca tacaaacccg cttacgacgc gctccaggga caggtgaaac
    10861 tgctgctgac cacctatttt gaaggcgtaa cgccaaatct cgacacgatt actgcgctgc
    10921 ctgttcaggg gctgcatgtg gaccttgtac atggtaaaga tgacgttgtt gaactgcaca
    10981 agcgcctgcc ttctgactgg ctgctgtctg cgggtctgat caatggtcgt aacgtctggc
    11041 gcgccgatct tactgagaaa tatgtgcaaa ttaaggacat tgtcggcaaa cgtgatttgt
    11101 gggtggcatc ttcctgctcg ttgctgcaca gccccatcga cctgagcgtg gaaacgcgtc
    11161 ttgatgcaga agtgaaaagc tggtttgcct tcgccctaca aaaatgccat gaactggcac
    11221 tgctgcgcga tgcgctgaac agtggtgaca cggcagctct ggcagagtgg agcgccccaa
    11281 ttcaggcgcg tcgtcactct actcgtgtac ataatccggc ggtagaaaag cgtctggcgg
    11341 cgatcaccgc ccaggacagc cagcgtgcga atgtctatga agtgcgtgct gaagcccagc
    11401 gtgcgcgttt taaactgcca gcgtggccga ccaccacgat tggttccttc ccgcaaacca
    11461 cggaaattcg taccctgcgt ctggatttca aaaagggcaa tctcgacgcc aataactacc
    11521 gcacgggcat tgcggaacat atcaggcagg ccattgttga acaggaacgt ttgggactgg
    11581 atgtgctggt acatggcgag gccgagcgta atgacatggt ggaatacttt ggcgagcacc
    11641 tcgacgggtt tgtctttacg caaaacggtt gggtacagag ctacggttcc cgctgcgtga
    11701 agccaccgat tgttattggt gacgttagtc gcccggcacc gattaccgtg gagtgggcga
    11761 agtatgcgca atctatgact gataaaccgg tgaaagggat gctgactggc ccggtgacca
    11821 ttctctgctg gtcgttcccg cgtgaagatg tcagccgtga aaccatcgcc aaacaaattg
    11881 cgctggcgct gcgtgatgaa gtggcggacc tggaagccgc aggaattggc atcatccaga
    11941 ttgatgaacc ggcgctgcgc gaaggtttac cgctgcgtcg tagcgactgg gatgcatatc
    12001 tccagtgggg cgtggaggcc ttccgtatca acgccgccgt ggcgaaagat gacacacaaa
    12061 tccacactca catgtgttat tgcgagttca acgacatcat ggattcgatt gcggcactgg
    12121 acgcagacgt catcaccatc gaaacctcgc gttctgacat ggagttgctg gagtcgttcg
    12181 aagagtttga ttatccgaat gaaatcggtc caggcgtcta tgacattcac tcgccaaacg
    12241 taccgagcgt ggaatggatt gaagccttgc tgaagaaagc ggcaaaacgc attccggcag
    12301 agcgcctgtg ggtcaacccg gactgtggcc tgaaaacgcg cggctggcca gaaacccgcg
    12361 cggcactggc gaacatggtg caggcggcgc agaacttgcg tcgggggtaa aatccaaacc
    12421 gggtggtaat accacccggt cttttctcat tacagcgact tcttcccacc atactgctta
    12481 aaccattcca gcatacgctg ccagccatct tctgcagatg cggcatgata gctcgggcga
    12541 taatcagcgt tgaatgcatg cccggcgtcc gggtacacga taatctctgc tttcgcatta
    12601 gcagcccgca gcgcctggcg catggtttca acgctctcct gcggaatact gttatccaga
    12661 ccgccatata agccgagaac cggcgcgtta agatcggttg cgatatcaac aggttgtttc
    12721 ggtgaattca gcgacttatc gcccgtcagt ttgccgtacc acgccactgc ggcttttagc
    12781 tgtggattat gcgcggcata cagccaagtg atacgtccac cccagcagaa tccggtgatc
    12841 attaaacgat gaacatcgcc gccgttgcgg gaagcccaac tggcgacatg atcgagatcg
    12901 gccagcacct gcgagtcagg cacttttgct accagaccgc taagcaacgt ggggatatcg
    12961 gcaaaatcat tcggatcgcc ttcgcggaag taaagttcag gtgcgatagc cagatacccc
    13021 tccagcgcca gacggcgaca aatgtcgcgg atatgttcat gcacgccaaa aatttcctgc
    13081 actacaatga ccactggcag tgggccatcg ctttgctttg gtctggcatg gtaagcaggc
    13141 atgttatccc cttgtgaagg gatagaggtg aagcccgcca caatcgcgtc gtccggggtc
    13201 tgaacgatgg tcgaagcgag aggcgatgca gcaggtgcaa atccagattg ttgtgttgtt
    13261 gccatggtat tctccgtacc cttataaaaa tgttgcgcaa tgttaactat agtcagcatg
    13321 caacaaatca cattgcctga atcggctcat cttttatgca gtcctgcaga atgaagagtg
    13381 atttatgtga tttgcatcac ctttggtggg taaatttatg caacgcattt gcgtcatggt
    13441 gatgagtatc acgaaaaaat gttaaaccct tcggtaaagt gtctttttgc ttcttctgac
    13501 taaaccgatt cacagaggag ttgtatatgt ccaagtctga tgtttttcat ctcggcctca
    13561 ctaaaaacga tttacaaggg gctacgcttg ccatcgtccc tggcgacccg gatcgtgtgg
    13621 aaaagatcgc cgcgctgatg gataagccgg ttaagctggc atctcaccgc gaattcacta
    13681 cctggcgtgc agagctggat ggtaaacctg ttatcgtctg ctctaccggt atcggcggcc
    13741 cgtctacctc tattgctgtt gaagagctgg cacagctggg cattcgcacc ttcctgcgta
    13801 tcggtacaac gggcgctatt cagccgcata ttaatgtggg tgatgtcctg gttaccacgg
    13861 cgtctgtccg tctggatggc gcgagcctgc acttcgcacc gctggaattc ccggctgtcg
    13921 ctgatttcga atgtacgact gcgctggttg aagctgcgaa atccattggc gcgacaactc
    13981 acgttggcgt gacagcttct tctgatacct tctacccagg tcaggaacgt tacgatactt
    14041 actctggtcg cgtagttcgt cactttaaag gttctatgga agagtggcag gcgatgggcg
    14101 taatgaacta tgaaatggaa tctgcaaccc tgctgaccat gtgtgcaagt cagggcctgc
    14161 gtgccggtat ggtagcgggt gttatcgtta accgcaccca gcaagagatc ccgaatgctg
    14221 agacgatgaa acaaactgaa agccaagcgg tgaaaatcgt ggtggaagcg gcgcgtcgtc
    14281 tgctgtaatt ctcttctcct gtctgaaggc cgacgcgttc ggccttttgt atttttgcgt
    14341 agcgcctcgc aggaaatgcc tttccaactg gacgtttgta cagcacaatt ctattttgtg
    14401 cgggtaagtt gttgcgtcag gaggcgttgt ggatttctca atcatggttt acgcagttat
    14461 tgcgttggtg ggtgtggcaa ttggctggct gtttgccagt tatcaacatg cgcagcaaaa
    14521 agccgagcaa ttagctgaac gtgaagagat ggtcgcggag ttaagcgcgg caaaacaaca
    14581 aattacccaa agcgagcact ggcgtgcaga gtgcgagtta ctcaataacg aagtgcgcag
    14641 cctgcaaagt attaacacct ctctggaggc cgatctgcgt gaagtaacca cgcggatgga
    14701 agccgcacag caacatgctg acgataaaat tcgccagatg attaacagcg agcagcgcct
    14761 cagtgagcag tttgaaaacc tcgccaaccg tatttttgag cacagcaatc gccgggttga
    14821 tgagcaaaac cgtcagagtc tgaacagcct gttgtcgccg ctacgtgaac aactggacgg
    14881 tttccgccgt caggttcagg acagcttcgg taaagaagca caagaacgcc ataccctgac
    14941 ccacgaaatt cgcaatctcc agcaactcaa cgcgcaaatg gcccaggaag cgatcaacct
    15001 gacgcgcgcg ctgaaaggcg acaataaaac ccagggcaac tggggcgagg tagtattgac
    15061 gcgggtgctg gaggcttccg gtctgcgtga agggtatgaa tatgaaaccc aggtcagcat
    15121 cgaaaatgac gcccgctcgc ggatgcagcc ggatgtcatc gtgcggctgc cgcagggaaa
    15181 agatgtggtg atcgacgcca aaatgacgct ggtcgcctat gaacgctatt ttaacgccga
    15241 agacgactac acccgcgaaa gcgcgctaca ggaacatatc gcgtcggtgc gtaaccatat
    15301 ccgtttgctg ggacgcaaag attatcaaca gctgccgggg ctgcgaactc tggattacgt
    15361 gctgatgttt attcccgttg aacccgcttt tttactggcg cttgaccgcc agccggagct
    15421 gatcaccgaa gcgttgaaaa acaatatcat gctggttagc ccgactacgc tgctggtggc
    15481 gctgcgcact atcgccaacc tgtggcgtta tgagcatcaa agccgcaacg cccagcaaat
    15541 cgccgatcgt gccagcaagc tgtacgacaa gatgcgtttg ttcatcgatg acatgtccgc
    15601 gattggtcaa agtctcgaca aagcgcagga taactatcgg caggcaatga aaaaactctc
    15661 ttcagggcgc ggaaatgtgc tggcgcaggc agaagcgttt cgcggtttag gagtagaaat
    15721 taaacgcgag attaatccgg atttggctga acaggcggtg agccaggatg aagagtatcg
    15781 acttcggtcg gttccggagc agccgaatga tgaagcttat caacgcgatg atgaatataa
    15841 tcagcagtcg cgctagccca ttgggagtag ttaagccggg tagaaatcta gggcatcgac
    15901 gcccaatctg ttacacttct ggaacaattt tttgatgagc aggcattgag atggtggata
    15961 agtcacaaga aacgacgcac tttggttttc agaccgtcgc gaaggaacaa aaagcggata
    16021 tggtcgccca cgttttccat tccgtggcat caaaatacga tgtcatgaat gatttgatgt
    16081 catttggtat tcatcgtttg tggaagcgat tcacgattga ttgcagcggc gtacgccgtg
    16141 ggcagaccgt gctggatctg gctggtggca ccggcgacct gacagcgaaa ttctcccgcc
    16201 tggtcggaga aactggcaaa gtggtccttg ctgatatcaa tgaatccatg ctcaaaatgg
    16261 gccgcgagaa gctgcgtaat atcggtgtga ttggcaacgt tgagtatgtt caggcgaacg
    16321 ctgaggcgct gccgttcccg gataacacct ttgattgcat caccatttcg tttggtctgc
    16381 gtaacgtcac cgacaaagat aaagcactgc gttcaatgta tcgcgtgctg aaacccggcg
    16441 gccgcctgct ggtgcttgag ttctcgaagc caattatcga gccgctgagc aaagcctatg
    16501 atgcatactc cttccatgtg ctgccgcgta ttggctcact ggtcgcgaac gacgccgaca
    16561 gctaccgtta tctggcagaa tccatccgta tgcatcccga tcaggatacc ctgaaagcca
    16621 tgatgcagga tgccggattc gaaagtgtcg actactacaa tctgacggca ggggttgtgg
    16681 cgctgcatcg tggttataag ttctgacagg agaccggaaa tgccttttaa acctttagtg
    16741 acggcaggaa ttgaaagtct gctcaacacc ttcctgtatc gctcacccgc gctgaaaacg
    16801 gcccgctcgc gtctgttggg caaagtgctg cgcgtggagg taaaaggctt ttcgacgtca
    16861 ttgattctgg tgttcagcga acgccaggtt gatgtactgg gcgaatgggc aggcgatgct
    16921 gactgcaccg ttatcgccta cgccagtgtg ttgccgaaac ttcgcgatcg ccagcagctt
    16981 gccgccctga ttcgcagtgg tgagctggaa gtgcagggcg atattcaggt ggtgcaaaac
    17041 ttcgttgcgc tggcaggtct ggcagagttc gaccctgcgg aactgctggc cccttatacc
    17101 ggtgatatcg ccgctgaagg aatcagcaaa gccatgcgcg gaggcgcaaa gttcctgcat
    17161 cacggcatta agcgccagca acgttatgtg gcggaagcca ttactgaaga gtggcgtatg
    17221 gcacccggtc cgcttgaagt ggcctggttt gcggaagaga cggctgccgt cgagcgtgct
    17281 gttgatgccc tgatcaaacg gctggaaaaa ctggaggcta aatgacgcca ggtgaagtac
    17341 ggcgcctata tttcatcatt cgcacttttt taagctacgg acttgatgaa ctgatcccca
    17401 aaatgcgtat caccctgccg ctacggctat ggcgatactc attattctgg atgccaaatc
    17461 ggcataaaga caaactttta ggtgagcgac tacgactggc cctgcaagaa ctggggccgg
    17521 tatggatcaa gttcgggcaa atgttatcaa cccgccgcga tctttttccg ccgcatattg
    17581 ccgatcagct ggcgttattg caggacaaag ttgctccgtt tgatggcaag ctggcgaagc
    17641 agcagattga agctgcaatg ggcggcttgc cggtagaagc gtggtttgac gattttgaaa
    17701 tcaagccact ggcttctgct tctatcgccc aggttcatac cgcgcgattg aaatcgaatg
    17761 gtaaagaggt ggtgattaaa gtcatccgcc cggatatttt gccggtcatt aaagcagacc
    17821 tgaaacttat ctaccgtctg gctcgctggg tgccgcgttt gctgccggat ggtcgccgtc
    17881 tacgcccaac cgaagtggtg cgcgagtacg aaaagacctt gattgatgaa ctgaatttgc
    17941 tgcgggaatc tgccaacgcc attcagcttc ggcgaaattt tgaagacagc ccgatgctct
    18001 acatcccgga ggtttaccct gactattgta gtgaagggat gatggtgatg gagcgtattt
    18061 acggcattcc ggtgtctgat gttgcggcgc tggagaaaaa cggcaccaac atgaaattgc
    18121 tggcggaacg cggcgtgcag gtgttcttca ctcaggtctt tcgcgacagc tttttccatg
    18181 ccgatatgca ccctggcaac atcttcgtaa gctatgaaca cccggaaaac ccgaaatata
    18241 tcggcattga ttgcgggatt gttggctcgc taaacaaaga agataaacgc tatctggcag
    18301 aaaactttat tgccttcttt aatcgcgact atcgcaaagt ggcagagcta cacgtcgatt
    18361 ctggctgggt gccaccagat accaacgttg aagagttcga atttgctatt cgtacggtct
    18421 gtgaacctat ctttgagaaa ccgctggccg aaatttcgtt tggacatgta ctgttaaatc
    18481 tgtttaatac ggcgcgtcgc ttcaatatgg aagtgcagcc gcaactggtg ttactccaga
    18541 aaaccctgct ctacgtcgaa ggggtaggac gccagcttta tccgcaactc gatttatgga
    18601 aaacggcgaa gcctttcctg gagtcgtgga ttaaagatca ggtcggtatt cctgcgctgg
    18661 tgagagcatt taaagaaaaa gcgccgttct gggtcgaaaa aatgccagaa ctgcctgaat
    18721 tggtttacga cagtttgcgc cagggcaagt atttacagca cagtgttgat aagattgccc
    18781 gcgagcttca gtcaaatcat gtacgtcagg gacaatcgcg ttattttctc ggaattggcg
    18841 ctacgttagt attaagtggc acattcttgt tggtcagccg acctgaatgg gggctgatgc
    18901 ccggctggtt aatggcaggt ggtctgatcg cctggtttgt cggttggcgc aaaacacgct
    18961 gattttttca tcgctcaagg cgggccgtgt aacgtataat gcggctttgt ttaatcatca
    19021 tctaccacag aggaacatgt atgggtggta tcagtatttg gcagttattg attattgccg
    19081 tcatcgttgt actgcttttt ggcaccaaaa agctcggctc catcggttcc gatcttggtg
    19141 cgtcgatcaa aggctttaaa aaagcaatga gcgatgatga accaaagcag gataaaacca
    19201 gtcaggatgc tgattttact gcgaaaacta tcgccgataa gcaggcggat acgaatcagg
    19261 aacaggctaa aacagaagac gcgaagcgcc acgataaaga gcaggtgtaa tccgtgtttg
    19321 atatcggttt tagcgaactg ctattggtgt tcatcatcgg cctcgtcgtt ctggggccgc
    19381 aacgactgcc tgtggcggta aaaacggtag cgggctggat tcgcgcgttg cgttcactgg
    19441 cgacaacggt gcagaacgaa ctgacccagg agttaaaact ccaggagttt caggacagtc
    19501 tgaaaaaggt tgaaaaggcg agcctcacta acctgacgcc cgaactgaaa gcgtcgatgg
    19561 atgaactacg ccaggccgcg gagtcgatga agcgttccta cgttgcaaac gatcctgaaa
    19621 aggcgagcga tgaagcgcac accatccata acccggtggt gaaagataat gaagctgcgc
    19681 atgagggtgt aacgcctgcc gctgcacaaa cgcaggccag ttcgccggaa cagaagccag
    19741 aaaccacgcc agagccggtg gtaaaacctg ctgcggacgc tgaaccgaaa accgctgcac
    19801 cttccccttc gtcgagtgat aaaccgtaaa catgtctgta gaagatactc aaccgcttat
    19861 cacgcatctg attgagctgc gtaagcgtct gctgaactgc attatcgcgg tgatcgtgat
    19921 attcctgtgt ctggtctatt tcgccaatga catctatcac ctggtatccg cgccattgat
    19981 caagcagttg ccgcaaggtt caacgatgat cgccaccgac gtggcctcgc cgttctttac
    20041 gccgatcaag ctgaccttta tggtgtcgct gattctgtca gcgccggtga ttctctatca
    20101 ggtgtgggca tttatcgccc cagcgctgta taagcatgaa cgtcgcctgg tggtgccgct
    20161 gctggtttcc agctctctgc tattttatat cggcatggcg ttcgcctact ttgtggtctt
    20221 tccgctggca tttggcttcc ttgccaatac cgcgccggaa ggggtgcagg tatccaccga
    20281 catcgccagc tatttaagct tcgttatggc gctgtttatg gcgtttggtg tctcctttga
    20341 agtgccggtg gcaattgtgc tgctgtgctg gatggggatt acctcgccag aagacttacg
    20401 caaaaaacgc ccgtatgtgc tggttggtgc attcgttgtc gggatgttgc tgacgccgcc
    20461 ggatgtcttc tcgcaaacgc tgttggcgat cccgatgtac tgtctgtttg aaatcggtgt
    20521 cttcttctca cgcttttacg ttggtaaagg gcgaaatcgg gaagaggaaa acgacgctga
    20581 agcagaaagc gaaaaaactg aagaataaat tcaaccgccc gtcagggcgg ttgtcatatg
    20641 gagtacagga tgtttgatat cggcgttaat ttgaccagtt cgcaatttgc gaaagaccgt
    20701 gatgatgttg tagcgcgcgc ttttgacgcg ggagttaatg ggctactcat caccggcact
    20761 aacctgcgtg aaagccagca ggcgcaaaag ctggcgcgtc agtattcgtc ctgttggtca
    20821 acggcgggcg tacatcctca cgacagcagc cagtggcaag ctgcgactga agaagcgatt
    20881 attgagctgg ccgcgcagcc agaagtggtg gcgattggtg aatgtggtct cgactttaac
    20941 cgcaactttt cgacgccgga agagcaggaa cgcgcttttg ttgcccagct acgcattgcc
    21001 gcagatttaa acatgccggt atttatgcac tgtcgcgatg cccacgagcg gtttatgaca
    21061 ttactggagc cgtggctgga taaactgcct ggtgcggttc ttcattgctt taccggcaca
    21121 cgcgaagaga tgcaggcgtg cgtggcgcat ggaatttata tcggcattac cggttgggtt
    21181 tgcgatgaac gacgcggact ggagctgcgg gaacttttgc cgttgattcc ggcggaaaaa
    21241 ttactgatcg aaactgatgc gccgtatctg ctccctcgcg atctcacgcc aaagccatca
    21301 tcccggcgca acgagccagc ccatctgccc catattttgc aacgtattgc gcactggcgt
    21361 ggagaagatg ccgcatggct ggctgccacc acggatgcta atgtcaaaac actgtttggg
    21421 attgcgtttt agagtttgcg gaactcggtg ttcttcacac tgtgcttaat ctctttatta
    21481 ataagattaa gcaatagcat ggagcgagcc tcaccatcgg gttcggtgaa aatggcctga
    21541 aagccttcga acgcgccttc ggtaataatc accttatcac ccggataagg ggttgccgga
    21601 tcgacaatgt ctttcggttt atataccgat agctgatgaa tcaccgccga tgggactatc
    21661 gctggcgacg cgccaaagcg cacgaagtgg ctgacaccgc gggtcgcgtt gatagtcgtg
    21721 gtatgaatca cttctggatc aaattccaca aacaggtagt tggggaacaa tggctcactg
    21781 actgcagtac gtttgccacg cacgattttt tccagggtga tcatcggtgc caggcaattc
    21841 acagcctgtc tttcgaggtg ttcctgggca cgttgaagtt gcccgcgctt gcagtacagt
    21901 aaataccagg attgcataat gactcttatc cgtttaatcg gggcgcaagg atagcaaaag
    21961 ctttacgcta agttaattat attccccggt ttgcgttata ccgtcagagt tcacgctaat
    22021 ttaacaaatt tacagcatcg caaagatgaa cgccgtataa tgggcgcaga ttaagaggct
    22081 acaatggacg ccatgaaata taacgattta cgcgacttct tgacgttgct tgaacagcag
    22141 ggtgagctaa aacgtatcac gctcccggtg gacccgcatc tggaaatcac tgaaattgct
    22201 gaccgcacgc tgcgtgctgg tgggcctgcg ctgttgtttg aaaaccctaa agggtactca
    22261 atgccggtgc tgtgcaactt gttcggtacg ccaaagcgcg tagcgatggg tatgggccag
    22321 gaagatgttt cagcactgcg tgaagtcggt aaattattag catttctgaa agaaccagag
    22381 ccgccaaaag gttttcgcga tctgtttgat aagctgccgc agtttaagca ggtgttaaac
    22441 atgccgacaa agcgactgcg cggtgcaccc tgccaacaaa aaatcgtctc tggcgatgac
    22501 gtcgatctca accgtattcc cattatgacc tgttggccgg aagatgccgc gccgctgatt
    22561 acatgggggc taaccgttac acgtggccct cataaagagc gacagaatct gggcatttat
    22621 cgccagcaac tgattggtaa aaacaagctg attatgcgtt ggctgtcgca tcgcggcggc
    22681 gcgctggatt atcaggagtg gtgtgcggcg catccaggtg aacgtttccc gatctctgtg
    22741 gcgttgggcg ctgatccggc aaccattctc ggtgcagtca caccagtacc agatactttg
    22801 tcggaatacg cctttgccgg attgctacgt ggcaccaaaa ccgaagtagt gaagtgtatt
    22861 tccaatgatc tcgaagtgcc cgccagtgcg gagattgtgc tggaagggta tatcgaacaa
    22921 ggcgaaatgg cgccagaagg accgtatggt gaccacactg gttactataa cgaagtcgat
    22981 agtttcccgg tatttaccgt gacgcatatt acccagcgtg aagatgcgat ttaccattcc
    23041 acctataccg ggcgtccgcc agatgaaccc gcggtactgg gagtggcgtt gaacgaagta
    23101 tttgttccca ttctgcaaaa gcagttcccg gaaattgtcg atttttacct gccgccggaa
    23161 ggctgctctt atcgcctggc ggtagtgaca atcaaaaaac agtacgccgg acacgcgaag
    23221 cgcgtcatga tgggcgtctg gtcgttctta cgccagttta tgtacactaa atttgtgatc
    23281 gtttgcgatg atgacgttaa cgcacgcgac tggaacgatg tgatttgggc gattaccacc
    23341 cgtatggacc cagcgcggga tactgttctg gtagaaaata cgcctattga ttatctggat
    23401 tttgcctcgc ctgtctccgg gctgggttca aaaatggggc tggatgccac gaataaatgg
    23461 ccgggggaaa cccagcgtga atggggacgt cccatcaaaa aagatccaga tgttgtcgca
    23521 catattgacg ccatctggga tgaactggct atttttaaca acggtaaaag cgcctgatgc
    23581 gcgtttgttt tgccctattt atcgatccga cagagaaagc gcatgacaac cttaagctgt
    23641 aaagtgacct cggtagaagc tatcacggat accgtatatc gtgtccgcat cgtgccagac
    23701 gcggcctttt cttttcgtgc tggtcagtat ttgatggtag tgatggatga gcgcgacaaa
    23761 cgtccgttct caatggcctc gacgccggat gaaaaagggt ttatcgagct gcatattggc
    23821 gcttctgaaa tcaaccttta cgcgaaagca gtcatggacc gcatcctcaa agatcatcaa
    23881 atcgtggtcg atattcccca cggagaagcg tggctgcgcg atgatgaaga gcgtccgatg
    23941 attttgattg cgggcggcac cgggttctct tatgcccgct cgattttgct gacagcgttg
    24001 gcgcgtaacc caaaccgtga tatcaccatt tactggggcg ggcgtgaaga gcagcatctg
    24061 tatgatctct gcgagcttga ggcgctttcg ttgaagcatc ctggtctgca agtggtgccg
    24121 gtggttgaac aaccggaagc gggctggcgt gggcgtactg gcaccgtgtt aacggcggta
    24181 ttgcaggatc acggtacgct ggcagagcat gatatctata ttgccggacg ttttgagatg
    24241 gcgaaaattg cccgtgacct gttttgcagt gagcgtaatg cgcgggaaga tcgcctgttt
    24301 ggcgatgcgt ttgcatttat ctgagatata aaaaaacccg cccctgacag gcgggaagaa
    24361 cggcaactaa aactttattc agtggcattt agatctatgt cgcatccggc attcggcaca
    24421 cgatgcctga tgcgacgctg gcgcgtctta tcaggcctac attggtgccg gatcggtaga
    24481 ccggataagg cgttcacgcc gcatccggca agtggttaaa cccgctcaaa caccgtcgca
    24541 ataccctgac ccagaccgat acacatcgtc gccagaccaa actgaacgtc tttgtgttcc
    24601 atcagattca gcagcgtggt gctgatacgc gcaccggaac aacccagcgg atgacccagc
    24661 gcgatcgcgc caccgttgag gttgatcttc tcgtcaatct gctcaattag tcccagatct
    24721 ttaatacatg gcaggatctg cgcggcaaag gcttcattca tttcaaacac gccgatatca
    24781 ctgacagaaa gccccgcttt tttcagcgcc agcttcgagg ccggaaccgg gccgtaaccc
    24841 atgatcgatg ggtcacaacc aacgaccgcc atcgaacgca cacgagcgcg cggcttaaga
    24901 cctaattcat gggcgcggct ttcactcatc accagcatgg cagctgcgcc atcggaaagt
    24961 gcagaagatg tgcccgccgt taccgtaccg tttactggat caaacgccgg acgcagcgtg
    25021 gcgagggctt ccacggtggt ttccgggcga atcacttcgt cgtaattaaa ctgcttcagg
    25081 acgccgtcgg catcgtgacc accagttggg atgatttcat ttttaaatgc gcccgactgc
    25141 gtggcggccc aggcgcgggc gtgtgaccgc gcggcaaagg catcctgcat ttcacggctg
    25201 ataccgtgca tacgcgccag catttctgcc gttaagccca tcatgcccgc cgctttggcg
    25261 acattgcggc tcaggccagg gtgaaaatcg acgccgtgac tcatcggcac atggcccata
    25321 tgctccacgc cgccaaccag acatgcctgc gcatcgccgg tcatgatcat tcgtgctgcg
    25381 tcatgcagtg cctgcatgga tgaaccacac aagcgattga cagtaaccgc cgggacagag
    25441 tgcggcactt ctgccagcag cgccgcgtta cgggcgatat taaaaccctg ctccagcgtc
    25501 tgctgcacac acccccagta aatatcgtcg agggccgccg cttccagcgc cgggttacgc
    25561 gccagcaggc tacgcattaa atgagcggag agatcttctg cacgcacgtt acgaaaagca
    25621 ccgcccttcg aacggcccat cggggtgcga attgcatcga caatgacaac ctgttccatt
    25681 gtgactcctt aagccgtttt caggtcgcca accggacggg ctggctcaac cgggggatag
    25741 tacggttcgt tatgacgcgc tttattacgc agaccttccg gcacttcgta cagcgggccg
    25801 aggtgctgat attgctgtgc catatcgagg tattttgcgc tgccgagggt gtccagccag
    25861 cggaacgcgc cgccgtggaa cggagggaag cccaggccgt agaccagcgc catatccgct
    25921 tctgccggag tggcgataat gccttcttcc agacagcgca ccacttcgtt gaccatcggg
    25981 atcatcatgc gggcgataat ctcttcttcg ctgaaatcgc gcttcggctg gctcacttct
    26041 gccagcaggt cgtcaacagc ggcgtcttct tctttcttcg gcttaccttt gctgtcttct
    26101 ttataatgcc agaaaccgag gccgttcttc tgaccaaagc ggttggcatc aaacagcgcg
    26161 tcgatggcat cgcgataatc tttctgcatc cgctgtggga agcccgctgc catgactgcc
    26221 tgggcgtgat gcgcggtgtc aatgcccaca acgtccagca gatatgccgg acccataggc
    26281 cagccaaact gtttttccat cactttgtcg atcttgcgga aatccgcgcc gtcgcgcagc
    26341 aactggctga aaccggcgaa atacgggaac agcacgcggt tgacgaagaa gccggggcaa
    26401 tcgttaacca caatcggcgt tttacccatc ttgctcgccc aggcgacgac tttcgcaata
    26461 gtttcgtcgg agcttttctc gccgcgaata atttccacca acggcattcg gtggaccggg
    26521 ttaaagaagt gcatcccgca gaagttttcc ggacgttcca gcgcgttggc cagttcgctg
    26581 ataggaatgg ttgaagtgtt agacgccagc acggtatccg ggcgtacttt ctgctcggtt
    26641 tctgccagca cggctttttt cactttcggg ttttcaacaa tcgcttctac cacaacatcc
    26701 acgcgctcaa atccggcgta gtcgagcgtt gggtggatcg tggagatcac gccagccagt
    26761 ttcagaccat cgatcttgcc gcgctcaagc tgcttgttca gtagtttcgc ggcttcagtc
    26821 atgccgaggg ctaacgactt gtcgttaata tctttcataa tcaccggcac gcctttccac
    26881 gcagactggt aagcgatgcc gccacccata atgcctgcgc ccagcacggc ggcctgtttc
    26941 ggggtttcaa cgtctttggt gagtttcttc gctttgcctt ttacatattg atcgttaagg
    27001 aaaatgccga ccagtgcgcg agcttcgttg gtatgcgcca gcgggacaaa gcttttgttt
    27061 tctaggttta aggcttcttc acgaccaaaa cgagccgcag cttcaatggt ttttactgcg
    27121 gtgatggggg ccgggtaatg ttttcctgcc gtttgtgcga ccatcccttt agcgatggtg
    27181 aagctcatgg tggcttcaat cttgctcagt tttagcggtt ccagcttcgg ctgacgtttc
    27241 gctttccagt cgaggtcgcc gttaatggcc tggcgtaaaa tcgccatcgc cccttcaatc
    27301 agtttttctg ctttgactac gccatccacc agaccgattt tcagcgcctg atccgcgccg
    27361 acatctttac cggcggcaat gatttccagc gcactgtcag cgcccagcat acgtggcata
    27421 cgtacagaac cgccaaagcc cggcatgatg cccagtttgg tttccggcag accgatgcgc
    27481 agatccggcg tcgccagacg gtaatcggtc gccagcacgc attcgcagcc accgcccagc
    27541 gcatagccat tgacggcagc aatggtcggc accggcagat cttccaggcg attaaacacg
    27601 ctattggcaa agtgcagcca ctgacttaac tgttcttcag gaacgaggaa cagggacaaa
    27661 aattcggtga tatcagcacc gacgataaag gctgctttgt tcgaacgcag cagcagccct
    27721 tttagatctg attgctgttc cagcacgccg atggcctcgc cgaggctggc gacggtcgca
    27781 gtgtcgagtt tattaactga acctggggca tcaaatacca gttcggcaat gccatcttcc
    27841 agccagtcaa ggtacagggt gtcgcctttg taaagcatgt cagtctcctg aatccgcaag
    27901 gtgatctggt cgtaccagat gcatcgaagt gtgtattttg tgttaaaaat atgcaaataa
    27961 aagattaaag aaatgccgat ctgatcacgc tcggcagaaa tcacgctctg gatgaacgat
    28021 gtgctaagat gcggagactt aaggtcaaaa aaacagaagg gtaaaaaatg gaatcactgg
    28081 cctcgctcta taaaaatcat atagctacat tgcaggaacg aactcgcgat gcgctgacgc
    28141 gcttcaagct ggatgcgtta cttattcact ccggcgaact gttcaacgtt tttctcgacg
    28201 atcatcccta tccgtttaaa gtgaacccgc aattcaaagc gtgggtgccg gtaactcagg
    28261 tgccaaactg ctggctgctg gtggatggcg tgaataagcc gaaactgtgg ttctatctgc
    28321 cggttgatta ctggcacaac gtcgaaccgc tgccgacctc cttctggact gaagatgtag
    28381 aagtgatcgc gctgccgaaa gccgatggca ttggtagtct gttgcctgct gcgcgcggca
    28441 atatcggtta tatcggtccg gtgccggaac gtgcgctgca actgggtatt gaggccagca
    28501 atatcaaccc gaaaggggtt atcgactacc tgcattacta ccgctccttc aaaaccgagt
    28561 acgagctggc ctgtatgcgt gaagcgcaga aaatggcggt caacggtcat cgcgcggcag
    28621 aagaagcgtt ccgttctggc atgagcgagt tcgatatcaa tattgcctat ctgactgcga
    28681 ccggtcatcg tgataccgac gtaccttaca gcaacattgt ggcactcaac gaacacgctg
    28741 cggtgctgca ttacaccaaa ctggatcatc aggcgtcgga agagatgcgc agcttcctgc
    28801 tggatgccgg ggccgaatat aacggctatg ccgctgacct gacccgtacc tggtcggcaa
    28861 aaagtgacaa cgattacgca cagctggtga aagacgtaaa tgatgaacaa ctggcgctga
    28921 tcgcgaccat gaaagctggc gttagctatg tggattacca catccagttc catcagcgca
    28981 tcgccaaatt gctgcgtaaa catcaaatca tcaccgatat gagtgaagag gcgatggtcg
    29041 aaaacgatct taccgggccg tttatgccgc atggtatcgg ccatccgctg ggcctgcagg
    29101 tgcatgacgt cgccggtttt atgcaggatg atagcggtac gcacctcgcg gcaccggcaa
    29161 aatatccgta cctgcgctgc acccgtattc tccagccggg catggtgtta accatcgaac
    29221 cgggtatcta cttcattgaa tcgctgctgg caccgtggcg tgaagggcag ttcagcaagc
    29281 acttcaactg gcagaaaatt gaagcactga aaccgttcgg cggcattcgt atcgaagaca
    29341 acgtggtgat ccacgaaaat aacgtggaaa acatgacccg ggatctgaaa ctggcgtgat
    29401 ggaaagctgg ttaattcctg cggcaccggt cacggtcgtt gaagagatca aaaaaagccg
    29461 cttcattacg ctgttggcgc ataccgatgg cgttgaggcg gcgaaagcgt ttgttgaatc
    29521 ggtgcgggca gaacaccccg atgcccgcca ccattgcgtg gcgtgggtgg cgggtgcgcc
    29581 ggatgattct caacagctgg gtttctctga cgacggggag ccggcgggaa cggcaggtaa
    29641 accgatgctc gcccagctaa tgggcagcgg cgttggggaa attaccgctg tggtggtgcg
    29701 ctactacggc ggcatattgc ttggcaccgg tgggttagtg aaagcgtatg gcggcggcgt
    29761 gaatcaggcg ctgcgccagc taacgaccca acgcaagacg ccattaaccg aatatacttt
    29821 gcaatgtgaa tatagtcagt taaccggcat tgaagcgttg ctggggcagt gtgacggcaa
    29881 aattatcaac agtgattatc aggcattcgt tctgctgcgg gtggcgcttc cggcggcgaa
    29941 agtggctgaa ttttccgcga agctggcgga ttttagccgt ggttcattgc aattgttagc
    30001 gattgaagaa taatccccac ttcgttttgc agaactaagg aagcggcaga gatgcatttt
    30061 cgcgccatta cccgaatcgt tggactactg gtcatcttat tttcagggac catgattatc
    30121 cctgggctgg tagcactcat ctaccgggat ggagcgggcc gcgcttttac ccagaccttt
    30181 tttgtcgccc tcgccattgg ctctatgctg tggtggccga accgcaaaga gaagggcgaa
    30241 ctgaaatccc gtgaggggtt tctgatagtg gtgctgttct ggaccgtgct gggtagtgtc
    30301 ggtgcgctcc cttttatctt ctcggaaagc ccgaacctca cgattaccga tgcgtttttt
    30361 gaatctttct ctggcctgac caccaccggg gccactacgc tggtggggct ggattcgctc
    30421 cctcatgcca tcctctttta tcgccagatg ctgcaatggt ttggcgggat ggggatcatc
    30481 gtgttagcgg ttgcgatact gcctatcctc ggcgtgggtg ggatgcagct ctatcgcgca
    30541 gaaatgcccg gcccgctgaa agataacaaa atgcgcccgc gaattgcgga aacggcgaaa
    30601 accctgtggt tgatttatgt cttgctgacc gtcgcctgtg cgctggcgtt gtggtttgcc
    30661 ggaatggatg cctttgatgc catcggccat agctttgcga ctatcgctat tggcggcttc
    30721 tcgacacatg atgccagtat cggttatttc gatagcccga ctattaacac tatcattgct
    30781 atcttcctgc tgatctccgg ctgtaactac ggtctgcact tttcactgtt aagtgggcgt
    30841 agtctgaagg tttattggcg cgatccggaa tttcgcatgt ttatcggcgt acagtttacg
    30901 ctggtggtta tttgtaccct cgtactgtgg tttcataatg tctacagttc ggcgctgatg
    30961 acaattaacc aggcgttttt ccaggtggtg tcgatggcga caaccgccgg gtttacgact
    31021 gacagcattg cccgctggcc gctctttttg ccggtactgc ttttatgttc agcttttatc
    31081 ggcggttgtg ccgggtcaac gggcggtggc ctgaaagtga tccgcatcct gctgctgttt
    31141 aagcagggga accgtgaact gaaacgactg gtgcatccga acgccgtcta tagcattaag
    31201 ctggggaatc gcgcactgcc ggaacgtatc ctcgaagccg tttggggatt tttctccgcc
    31261 tatgcattgg tgtttattgt cagtatgctg gcgattatcg ccacgggcgt ggatgacttt
    31321 tctgcctttg cctcggttgt tgcgacattg aataacctgg ggccagggct tggcgtggtt
    31381 gctgataact ttaccagtat gaacccggtg gctaaatgga tcctgattgc caacatgctg
    31441 tttggtcgtc tcgaggtctt tacattgctg gtgctcttta ccccgacttt ctggcgtgaa
    31501 tgatggagta atacgtgaaa acattaattc ttttctcaac aagggacgga caaacgcgcg
    31561 agattgcctc ctacttggct tcggaactga aagaactggg gatccaggcg gatgtcgcca
    31621 atgtgcaccg cattgaagaa ccacagtggg aaaactacga ccgtgtggtc attggtgctt
    31681 ctattcgcta tggtcactac cactctgcgt tccaggaatt tgtcaaaaag catgcgacgc
    31741 ggctgaattc gatgccgagc gcgttttact ccgtaaacct ggtggcgcgt aaaccggaga
    31801 agcgcactcc acagaccaac agctacgcgc ggaagtttct gatgaactcg caatggcgtc
    31861 ccgatcgctg cgcggtcatt gccggggcgc tgcgttaccc acgttatcgc tggtacgacc
    31921 gttttatgat caagctgatt atgaagatgt caggcggtga aacggatacg cgcaaagaag
    31981 ttgtctatac cgattgggag caggtggcga atttcgcccg agaaatcgcc catttaaccg
    32041 acaaaccgac gctgaaataa gcataaagaa taaaaaatgc gcggtcagaa aattatttta
    32101 aatttcccct tgtcaggccg gaataactcc ctataatgcg ccaccactga cacggaacaa
    32161 cggcaaacac gccgccgggt cagcgggatt ctcctgagaa ttccg
//
LOCUS       DABSYT020000048        32016 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.048, whole genome shotgun sequence.
ACCESSION   DABSYT020000048 DABSYT020000000
VERSION     DABSYT020000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.048"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(27..496)
                     /locus_tag="ID725_RS13175"
                     /old_locus_tag="pgaptmp_002635"
                     /pseudo
     CDS             complement(27..496)
                     /locus_tag="ID725_RS13175"
                     /old_locus_tag="pgaptmp_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(1155..1337)
                     /locus_tag="ID725_RS13180"
                     /old_locus_tag="pgaptmp_002636"
     CDS             complement(1155..1337)
                     /locus_tag="ID725_RS13180"
                     /old_locus_tag="pgaptmp_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851212.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            1276..3366
                     /gene="iha"
                     /locus_tag="ID725_RS13185"
                     /old_locus_tag="pgaptmp_002637"
     CDS             1276..3366
                     /gene="iha"
                     /locus_tag="ID725_RS13185"
                     /old_locus_tag="pgaptmp_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGV7851213.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(3584..3743)
                     /locus_tag="ID725_RS13190"
                     /old_locus_tag="pgaptmp_002638"
                     /pseudo
     CDS             complement(3584..3743)
                     /locus_tag="ID725_RS13190"
                     /old_locus_tag="pgaptmp_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3946..4266)
                     /locus_tag="ID725_RS13195"
                     /old_locus_tag="pgaptmp_002639"
                     /pseudo
     CDS             complement(3946..4266)
                     /locus_tag="ID725_RS13195"
                     /old_locus_tag="pgaptmp_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(4689..5264)
                     /locus_tag="ID725_RS13200"
                     /old_locus_tag="pgaptmp_002640"
     CDS             complement(4689..5264)
                     /locus_tag="ID725_RS13200"
                     /old_locus_tag="pgaptmp_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGV7851214.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(5333..5911)
                     /gene="terD"
                     /locus_tag="ID725_RS13205"
                     /old_locus_tag="pgaptmp_002641"
     CDS             complement(5333..5911)
                     /gene="terD"
                     /locus_tag="ID725_RS13205"
                     /old_locus_tag="pgaptmp_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HGV7851215.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(5960..7000)
                     /gene="terC"
                     /locus_tag="ID725_RS13210"
                     /old_locus_tag="pgaptmp_002642"
     CDS             complement(5960..7000)
                     /gene="terC"
                     /locus_tag="ID725_RS13210"
                     /old_locus_tag="pgaptmp_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HGV7851216.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(7023..7478)
                     /locus_tag="ID725_RS13215"
                     /old_locus_tag="pgaptmp_002643"
     CDS             complement(7023..7478)
                     /locus_tag="ID725_RS13215"
                     /old_locus_tag="pgaptmp_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGV7851217.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(7501..8658)
                     /locus_tag="ID725_RS13220"
                     /old_locus_tag="pgaptmp_002644"
     CDS             complement(7501..8658)
                     /locus_tag="ID725_RS13220"
                     /old_locus_tag="pgaptmp_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGV7851218.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(8658..9239)
                     /locus_tag="ID725_RS13225"
                     /old_locus_tag="pgaptmp_002645"
     CDS             complement(8658..9239)
                     /locus_tag="ID725_RS13225"
                     /old_locus_tag="pgaptmp_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGV7851219.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            9562..10620
                     /locus_tag="ID725_RS13230"
                     /old_locus_tag="pgaptmp_002646"
     CDS             9562..10620
                     /locus_tag="ID725_RS13230"
                     /old_locus_tag="pgaptmp_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGV7851220.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            10630..11772
                     /locus_tag="ID725_RS13235"
                     /old_locus_tag="pgaptmp_002647"
     CDS             10630..11772
                     /locus_tag="ID725_RS13235"
                     /old_locus_tag="pgaptmp_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HGV7851221.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            11765..12538
                     /locus_tag="ID725_RS13240"
                     /old_locus_tag="pgaptmp_002648"
     CDS             11765..12538
                     /locus_tag="ID725_RS13240"
                     /old_locus_tag="pgaptmp_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851222.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            12540..13619
                     /locus_tag="ID725_RS13245"
                     /old_locus_tag="pgaptmp_002649"
     CDS             12540..13619
                     /locus_tag="ID725_RS13245"
                     /old_locus_tag="pgaptmp_002649"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGV7851223.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            13619..14575
                     /locus_tag="ID725_RS13250"
                     /old_locus_tag="pgaptmp_002650"
     CDS             13619..14575
                     /locus_tag="ID725_RS13250"
                     /old_locus_tag="pgaptmp_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGV7851224.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            14586..15794
                     /locus_tag="ID725_RS13255"
                     /old_locus_tag="pgaptmp_002651"
     CDS             14586..15794
                     /locus_tag="ID725_RS13255"
                     /old_locus_tag="pgaptmp_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGV7851225.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            15812..16279
                     /gene="terW"
                     /locus_tag="ID725_RS13260"
                     /old_locus_tag="pgaptmp_002652"
     CDS             15812..16279
                     /gene="terW"
                     /locus_tag="ID725_RS13260"
                     /old_locus_tag="pgaptmp_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HGV7851226.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            16540..16869
                     /locus_tag="ID725_RS13265"
                     /old_locus_tag="pgaptmp_002653"
     CDS             16540..16869
                     /locus_tag="ID725_RS13265"
                     /old_locus_tag="pgaptmp_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGV7851227.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            16856..17197
                     /gene="tnpB"
                     /locus_tag="ID725_RS13270"
                     /old_locus_tag="pgaptmp_002654"
     CDS             16856..17197
                     /gene="tnpB"
                     /locus_tag="ID725_RS13270"
                     /old_locus_tag="pgaptmp_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGV7851228.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            17279..18109
                     /locus_tag="ID725_RS13275"
                     /old_locus_tag="pgaptmp_002655"
                     /pseudo
     CDS             17279..18109
                     /locus_tag="ID725_RS13275"
                     /old_locus_tag="pgaptmp_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(18140..19753)
                     /locus_tag="ID725_RS13280"
                     /old_locus_tag="pgaptmp_002656"
     CDS             complement(18140..19753)
                     /locus_tag="ID725_RS13280"
                     /old_locus_tag="pgaptmp_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HGV7851229.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(19784..20134)
                     /gene="tnpB"
                     /locus_tag="ID725_RS13285"
                     /old_locus_tag="pgaptmp_002657"
     CDS             complement(19784..20134)
                     /gene="tnpB"
                     /locus_tag="ID725_RS13285"
                     /old_locus_tag="pgaptmp_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGV7851230.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(20131..20556)
                     /locus_tag="ID725_RS13290"
                     /old_locus_tag="pgaptmp_002658"
     CDS             complement(20131..20556)
                     /locus_tag="ID725_RS13290"
                     /old_locus_tag="pgaptmp_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGV7851231.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            20610..21361
                     /locus_tag="ID725_RS13295"
                     /old_locus_tag="pgaptmp_002659"
                     /pseudo
     CDS             20610..21361
                     /locus_tag="ID725_RS13295"
                     /old_locus_tag="pgaptmp_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            21701..21874
                     /locus_tag="ID725_RS13300"
                     /old_locus_tag="pgaptmp_002660"
     CDS             21701..21874
                     /locus_tag="ID725_RS13300"
                     /old_locus_tag="pgaptmp_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851232.1"
                     /translation="MSSPEQYLSSSEPASDTSLAVAPAQQNKSTIVRLRSFVCIADDA
                     PEKRVNMSSPEYY"
     gene            22894..23565
                     /locus_tag="ID725_RS13305"
                     /old_locus_tag="pgaptmp_002661"
     CDS             22894..23565
                     /locus_tag="ID725_RS13305"
                     /old_locus_tag="pgaptmp_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851233.1"
                     /translation="MEFSVIFFWVITGLITILFLKSIITDLIKNPNKTDIALYILAFL
                     SAGYTFQYIYNDNIFSELWGVGLFVTLIVLNIVFNLFFRLLFILSKMILMSRHIRKMQ
                     KKLKQTSVPDFIYYSIEKHNKYPIYVLHMPSNNIIEIGYNIINTDLVIGEKIHFRTLS
                     NRSLYFNLTHPPLIATIKDDVFCTLHDYYNHNNETKSTIDNYISKIKDNHNTPWLLNN
                     ENVQE"
     gene            complement(24436..24576)
                     /locus_tag="ID725_RS13310"
                     /old_locus_tag="pgaptmp_002662"
     CDS             complement(24436..24576)
                     /locus_tag="ID725_RS13310"
                     /old_locus_tag="pgaptmp_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGV7851234.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(24878..25141)
                     /locus_tag="ID725_RS13315"
                     /old_locus_tag="pgaptmp_002663"
     CDS             complement(24878..25141)
                     /locus_tag="ID725_RS13315"
                     /old_locus_tag="pgaptmp_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGV7851235.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            25566..25691
                     /locus_tag="ID725_RS13320"
                     /old_locus_tag="pgaptmp_002664"
                     /pseudo
     CDS             25566..25691
                     /locus_tag="ID725_RS13320"
                     /old_locus_tag="pgaptmp_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            25756..26016
                     /locus_tag="ID725_RS13325"
                     /old_locus_tag="pgaptmp_002665"
                     /pseudo
     CDS             25756..26016
                     /locus_tag="ID725_RS13325"
                     /old_locus_tag="pgaptmp_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            26066..26305
                     /locus_tag="ID725_RS13330"
                     /old_locus_tag="pgaptmp_002666"
                     /pseudo
     CDS             26066..26305
                     /locus_tag="ID725_RS13330"
                     /old_locus_tag="pgaptmp_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(26353..26970)
                     /gene="ureG"
                     /locus_tag="ID725_RS13335"
                     /old_locus_tag="pgaptmp_002667"
     CDS             complement(26353..26970)
                     /gene="ureG"
                     /locus_tag="ID725_RS13335"
                     /old_locus_tag="pgaptmp_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HGV7851236.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(26982..27656)
                     /locus_tag="ID725_RS13340"
                     /old_locus_tag="pgaptmp_002668"
     CDS             complement(26982..27656)
                     /locus_tag="ID725_RS13340"
                     /old_locus_tag="pgaptmp_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HGV7851237.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(27657..28121)
                     /gene="ureE"
                     /locus_tag="ID725_RS13345"
                     /old_locus_tag="pgaptmp_002669"
     CDS             complement(27657..28121)
                     /gene="ureE"
                     /locus_tag="ID725_RS13345"
                     /old_locus_tag="pgaptmp_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HGV7851238.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(28131..29834)
                     /gene="ureC"
                     /locus_tag="ID725_RS13350"
                     /old_locus_tag="pgaptmp_002670"
     CDS             complement(28131..29834)
                     /gene="ureC"
                     /locus_tag="ID725_RS13350"
                     /old_locus_tag="pgaptmp_002670"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HGV7851239.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(29827..30147)
                     /locus_tag="ID725_RS13355"
                     /old_locus_tag="pgaptmp_002671"
     CDS             complement(29827..30147)
                     /locus_tag="ID725_RS13355"
                     /old_locus_tag="pgaptmp_002671"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HGV7851240.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(30156..30458)
                     /locus_tag="ID725_RS13360"
                     /old_locus_tag="pgaptmp_002672"
     CDS             complement(30156..30458)
                     /locus_tag="ID725_RS13360"
                     /old_locus_tag="pgaptmp_002672"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HGV7851241.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(30549..31247)
                     /locus_tag="ID725_RS13365"
                     /old_locus_tag="pgaptmp_002673"
     CDS             complement(30549..31247)
                     /locus_tag="ID725_RS13365"
                     /old_locus_tag="pgaptmp_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HGV7851242.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            31628..31903
                     /locus_tag="ID725_RS13370"
                     /old_locus_tag="pgaptmp_002674"
     CDS             31628..31903
                     /locus_tag="ID725_RS13370"
                     /old_locus_tag="pgaptmp_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851243.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         8064 a         8106 c         7962 g         7884 t
ORIGIN      
        1 gcatttcaac aatatatact tgttaaaaat ccatatcgaa atgccgttgc agcattaata
       61 tatgcctcat tcataaaatg taaaagagca agctcgtacc agtagggggg aaattcattg
      121 acatgtcctg tcaatacgta cagagccctg ccatgcttgc cagcacgtaa caattccgcc
      181 cccagtaacc agcgtgttcc ctgattattc tcagggttat atgttagtat tttatcaatg
      241 agtataacgg catcctgatg acgctgtaaa tgaacattcg ccagaattgc agcataaaga
      301 gcgcaaatgc atatgccaga tgagcgtgaa tatcaatgat gtcaggagca tgtcaggcaa
      361 gacgcctgag tttgttgacg taatttgttt cgtttatttc atcactatcg tatgcgtcaa
      421 gagttttttc aaactcatcc cgaaatgggc caagcctggt ttcaggaaat aagaaatacc
      481 cttggttatt gttcacttaa aagtataatt tgtaattcaa atcctgagcc tgtaaagcgg
      541 ggagtaacat attctattct gaagagaata aaagtcgtga tgcgatgtat caagcccgga
      601 ttgtaatccc agattaacat agatcacaaa acaacttatt cttcactaac gtcaagataa
      661 atatcgttgt atgccttatc acgactacga ataccagcaa gatacaactg acatacggaa
      721 agataccacg ttttttactc ccgaaaataa cgctaaaaag ctacttcccc atcgtttgtc
      781 cttagtattg ccagcgccaa caatgtgggc tgacatgata aagctgtcta ggaaattgtt
      841 cgcctcctca gcggacaatc caaatggtga ttgtctctgt taaacgttta ttttgaaggt
      901 cgactgaata aggtgatgac gctgtagaat ttttcacgtg ccacagaatt ttgaacgctt
      961 tctcttacaa tatttcaatg tttctatcag tattcgccgg aagaagtcat cgaccaaatc
     1021 atcccagtcg tctcgcatca ctgaccattc atggttgaca tgtggtggaa atccgcttct
     1081 acagtaacca ttttttattc gcaaaaccga acacgccata cggatagctg ttaactggca
     1141 tgccgaggca gtcgttattt atatttggtt ttgtcaataa tctttatttt ttgtaaaagg
     1201 caaatataaa ttattctcat tattgtttgt atttgtgtat tgtcttgccg gttaacatga
     1261 tcggagatta gtaatatgcg aataaccact ctggcttccg tagtcattcc ctgtctcgga
     1321 ttttcagcca gcagcatagc tgctgcagag gatgtgatga ttgtctcggc atccggctat
     1381 gagaaaaagc tgactaacgc agccgccagt gtttctgtga ttagccagga ggaattgcag
     1441 tccagccagt accacgatct ggcggaggct ctgagatcag tagagggtgt ggatgttgaa
     1501 agtggtacgg gtaaaaccgg agggctggaa atcagcatcc gaggaatgcc agccagttac
     1561 acgctgatac tgattgatgg tgttcgtcag ggcggaagca gtgacgtgac tcccaacggt
     1621 ttttctgcca tgaataccgg gttcatgccc cctctggccg ccattgagcg tattgaggtt
     1681 atcagggggc cgatgtccac actgtatggc tctgatgcga tgggcggtgt ggtgaatatc
     1741 attaccagaa agaatgcaga caaatggctc tcttccgtca atgcagggct gaatctgcag
     1801 gaaagcaaca aatggggtaa cagcagccag tttaatttct ggagcagtgg tccccttgtg
     1861 gatgattctg tcagcctgca ggtacgcggt agcacacaac agcgtcaggg ttcatcggtc
     1921 acatcactga gcgatacagc agccacgcgt attccttatc ccacggagtc acagaattat
     1981 aatcttggtg cacgtcttga ctggaaggcg tcggagcagg atgtgctctg gtttgatatg
     2041 gataccaccc ggcagcgtta tgataaccgg gatgggcaac tggggagtct gacgggggga
     2101 tatgaccgga ccctgcgcta tgagcgaaac aaaatttcag ctggctatga tcatactttc
     2161 accttcggaa catggaaatc gtatctgaac tggaacgaga cagaaaataa aggtcgtgag
     2221 cttgtacgca gtgtactgaa gcgcgacaaa tgggggcttg ccggtcagcc gcgggagctt
     2281 aaggaatcga accttatcct gaattcatta ctgcttaccc ctctgggaga atctcatctg
     2341 gttacggtgg ggggcgagtt tcagagctcg tccatgaaag acggagttgt ccttgccagc
     2401 acaggtgaaa ctttccggca gaaaagctgg tcggtatttg ctgaggatga gtggcatctc
     2461 acggatgcac ttgcgctgac tgcgggcagc cgctatgaac atcatgagca attcggggga
     2521 cacttcagtc cgcgtgcata tctggtctgg gatgtggcag atgcctggac gctgaaaggc
     2581 ggtgtgacca cgggatataa ggcacccaga atggggcagc tacataaagg gattagtggt
     2641 gtgtccgggc agggaaaaac aaatctactt ggtaaccccg acctgaagcc ggaagagagc
     2701 gtcagttatg aggctggggt gtattacgat aaccccgccg gtctgaatgc caatgtcaca
     2761 ggttttatga ctgacttctc caacaagatt gtctcttatt ccataaatga taacaccaat
     2821 agctatgtaa acagcggaaa ggcccggttg cacggtgtgg aatttgccgg cacattgccg
     2881 ctgtggtcag aggatgtcac gctgtcactg aattacacct ggacccgaag tgaacaacgt
     2941 gatggtgata acaaaggtgc gccgctgagt tatacccctg aacacatggt gaatgcgaaa
     3001 ctgaactggc agatcaccga agaggtggca tcatggctgg gtgcccgtta tcgcgggaaa
     3061 acaccacgtt tcacccagaa ttattcgtca ctgagcgctg tacagaagaa agtgtatgat
     3121 gagaaaggag aatacctgaa agcctggacg gtggtggatg caggtctgtc gtggaagatg
     3181 acggatgccc tgacgctgaa tgctgcggtg aataacctgc tcaacaagga ttacagtgac
     3241 gtgagcctgt acagtgccgg taagagtacg ctgtatgccg gtgattactt ccagacggga
     3301 tcatcaacaa caggatatgt gatacctgag cgaaattact ggatgtcgct gaactatcag
     3361 ttctgataat aacaaaacgc tatcactgac ggtagaatac gttgccactg caactcctgg
     3421 cggaacagtg gcaacgtatt aggttaagtg catttccgat ccgctaatga gatttcgtta
     3481 ccaacaacta atatcgtcac aggaaatgca cggattattt ttaacttatc atttacatac
     3541 ttgtccagag tgtaagcgca ccgtgaagga cgtggggtaa aaattagttt acagagagag
     3601 tgacgttcca gggcaacaac tctttcatgc ggttggcagg ccaggtgttg attacactga
     3661 tcacgtggcg tagccacgtt tccggctcga ttccgttaag ttttgcagct accgatcagg
     3721 ctgtacatca ctgccgcact atcgctcgtc atctcaaagt ccggtctcgt cagcaggaag
     3781 gtatcattct ctcccgccat ttttccaggg gcccggtcag ataagtccct ttgtctatcg
     3841 ctgactcctg actcataacc cggttagcag aatgcagggt caccacctcg ccacgaccaa
     3901 atccaaataa gtcaattgca ccttctcaat cgccattttg tcagtaagcg tacagcctga
     3961 actgatggta tcttcaccat caatgacaac ggtgatcgca attttactga cgttcgccgg
     4021 aacacgatcc agtgctatct caatgctggc ctgctgcgaa ccggtaacga gcctgactgc
     4081 cccctcagga gaagacaaat tattataaaa gataaagtca gaatcgccac tgacctttcc
     4141 ctgagcatta agcatgaaca ggcaggtatc gggttcgcct ttaaagccgg atttggcagg
     4201 gtactgaaga ttcagcctga tcgtagattg ctgaaggggt atgttttgtc cggattgtaa
     4261 attcatatta actctcctga tttttgatta ttattaatgc gcagcgttta tatatgttcc
     4321 ctaggcttag ttctggacgc tggatattcg gtgaggcgta aatatggtat gacaccattt
     4381 ttcataacgc tgaagtttct atacctgttg aatttgaatt ttcattgacc gggtatctta
     4441 ttttccaggg cccgttcctt cataagtcgc aaaagtaaca tatatccgaa gggcatgctg
     4501 ttgatatcag acacggaata ctggctttaa ccagcaacca cagataaacc cggggcctgc
     4561 ataaaaggtt catgccagaa ttaacatagc tctgctttct gccccccccc tttcccatat
     4621 tcacccgttg atagcggatc gatacccaaa aaaaaccccg gcattgccgg ggtcagactc
     4681 agcgtctttt agacattgac gccgtgctgg gaagcaagcg ctgacagacc accggcaaaa
     4741 ccctggccaa cagctttaaa cttccactca gtaccatggc gatacagttc accgaagacc
     4801 attgcggttt cggttgaggc gtcttcagac agatcgaaac gggcaatttc cgtcccgttg
     4861 tcgttgttgt aaacgcgcat gaagctgttg ctcaccatgc cgaagttttg tttacgcgct
     4921 tctgcatcat agatggtaac ggcaaatacc agttttttga tgtctgctga gactttggtc
     4981 agatcgattt tgacctgctc atcgtcgccg tcgccttcac cggtacggtt gtcgccctgg
     5041 tgctctactg cgccatcagg gctggtttta ttattgaaga aaatgaaatg ggcatctgac
     5101 agtactttac cgtcttcacc tactgcgaat acggaagcgt ccagatcaaa accctgacca
     5161 tcggttacac gggcatccca gcccaggcca accatagcga cattcatggt tggtgcttct
     5221 ttggtcagag atacgttgcc gccttttacg agagaaactg ccatttttag ctcctgcaaa
     5281 cagttgaatg aggctgaatt aacagcccca gatatgaaaa gttacttttc gatcaggacg
     5341 cgttaatgcc gtactgagca catacagatg ccagaccacc agcataaccc tggcctactg
     5401 cgcggaattt ccactcacca ttgtggcgat acagctcgcc gaacagcatg gcagtctcag
     5461 tggacgcatc ttcggtcaga tcgtagcgag cgacttcagt ctggttatcg tcattaacca
     5521 gacgaataaa cgcaccggat acctgaccaa agctctggcg acgagcctga gcatcgtgga
     5581 tggtcacaac gaagatgatc ttgtcaactt cagacgggac ggcgtccagt ttaattttca
     5641 gcgattcatc atcaccatcg ccctcaccgg tgcggttatc gccggtgtgc gttacggaac
     5701 cgtcggatga cgtcaggttg ttatagaaga tgaaatctga atcgccgcgc actttgccgt
     5761 ttgaggccag caggaatgct gaagcatcca ggtcaaagtc ctgaccgtct gttgaacgcg
     5821 catcccagcc aaggcccacc aggacatttt tcattgacgg agctgcttta ctcagggaga
     5881 cgttcccgcc tttggaaaga gaaacactca taaaataacc tcttcgatta gtaattgttc
     5941 aggttaacac ttaaggggat taactcccct tttcctcaga ttcaggtttg cccgggaaca
     6001 tgacgcttgc gagaatgccc agcgccagta cacccagtac aacatacagg ctggttgttg
     6061 ccgcgatgct gtaaccatga tgccagatgt gatcggtcgc attcaggccg agttttgccg
     6121 cgatgaagaa cagcagcacg ataacggcct tctccagatg aaccaggtac tgtttcagtg
     6181 cctcaaggac aaaatacaga gtacgcagac ccaggatagc aaacatcatg gcactataga
     6241 cgataagcgg ttcacgactg acggcaatga ttgccggtac cgagtcaaac gcgaacatta
     6301 cgtccgagag ttcaaccaca gccacacaca ggaacagcgg ggtcgcatac agcgccgctt
     6361 ttttaccacg gccaatggtg acatcgctgt tttctggttt cgccagttca gcatccactt
     6421 ccttctggtt aagtaggaag gcatgccctc tgagcttcgg ccagataggg aagaagcgtt
     6481 taaccatacg gtaagccaga tgctgggagt aatcctcaat ttcatcatcg tcatcaccgc
     6541 ttttaagcat catgaccgcc gtccaggcaa cgataatagc gaagacaact tcaacgtacg
     6601 gccccagact cagcaggctc gtaccgatgg cgacaaagat gcccctgaag acgatggcac
     6661 caatgatccc ccagtagaga acacggtggc gataacgatc cggaacggcg aaccaggaga
     6721 agattgccat catgacgaac aggttatcga ccgacagcac tttctccagc gcataacccg
     6781 tgacaaacag actggcaacc tcagcaccgt ggtggatata gaggaaaccg gcaaatgcca
     6841 tcgcaaccac aacccagaat acggaccaga gcgcagcact cttcagcgag attggcttgt
     6901 catcacggtg cataaacagg tcaataaaga tggcaccgac tgaaagcgca ataaaaacaa
     6961 tgaccgtttc agtcgggaag ccgatgtgtg tggaaaccat attttaccct tagggatgtg
     7021 gatcagaggc caaattcagc cgggtcgaag cccaacgcga tagcgatctc gcgggaggcc
     7081 agtttctcgt cctgatcgaa gttaccgtca cttttcgcaa cggcaatacc aacacgtaag
     7141 gccagctgag cggcctcagg ctgatctttc agcgccagga tgtatttcat ggtttcgccc
     7201 ttgccaattt caacatcgaa atcgaagctt gaaaccagtt tattgaagaa ctcaatcacc
     7261 tcattggtat cgaagacctt cagctcttct gaagagcgca gaaagcccat cattttctgc
     7321 ttttcttccg aacttacgcc gtcactcgat acggcaatac gggcacatac agcaacggtg
     7381 ccctgcatga attttttgtt tttgaaacgg ccaacctggc gggtcagttc gtcacggcct
     7441 gagttaatgg cacctttaac tttgtcgaaa aagctcatac ctgtttcctt tgttgctgtg
     7501 ttatttagaa ccggcgctcc agcgaaatcc ccagccaaat gcccggtcca tttcgtcctg
     7561 gcctttgaag tactggttaa ttcgctcgac tttaattgcg ccgttttcgt ttacgagtct
     7621 ggcaatggcg cacaatgtgc gacggttctc accttcggtc aggcgggtct caatgggcgg
     7681 ttgatccggc acatgaatgg tgactacacc atcagtcttg tcccagctgg gaacaccttc
     7741 ataaataaag gcgtaaatca gcacttcgcg gatatgcttc cattcacgtc cattgatatg
     7801 cagccactcg ccgtctgata catcacccgt ccggtcatcg cccttgagct gaacatacgg
     7861 ttcatcgcga taatcaccga atgcattacc gagcgcctga atgaccgatt tatacccgtc
     7921 ctgaagctca acaaaggccc ccagatccag gtcgattcct ttggagccga acatacctgc
     7981 aaagcctgat ttgctactgc cccggtgcca gttcaggttg atccggattt caccgaagtt
     8041 atcccgtttg gtcaggctga ttgcaggctt ctcttttgtc agcgatacct tgctcaggct
     8101 gatttttgtc tcaactggcg gaggggttac aaccggagga gttggggctg cgggagccgg
     8161 ttcgtcggca atatttacgc cgaaatgctc tgccagcggt ttaagtccac cgttgaaccc
     8221 ctgcgcaacg aagcggaatt tccagtcatt attacgacgg taaaattccc ccagaattaa
     8281 ggctgcttcc tgacggccgc tcagttcgac actgccgcta accaggccgg tagccccttg
     8341 ctccacgtcg atggaaagat tacgaagacc ggacaccgtc tgcccaccgt cacaggtcac
     8401 ggtgaacgca attttctgga catcaggttt cagccggttg agcgcgacag taaaggttga
     8461 gtactggcct tcactgacca ggctgacggt accatcatcg ttacgtggct gaccataaaa
     8521 caccatgtcc gcatcaccct gcaccttccc gtcggcatac aggcgaaacg ccgaagcatc
     8581 cacctggccg cccgaggtga tccggacccg caactcctgg gctggaacgg gggcattgcc
     8641 ccccggcgtc aggttcatta gcgaataacc gctgaaacga tatcggagtg catatcgtca
     8701 attgtgcggc cgcgacaggc atggccaagg gcggtaaagt cccagttgcc gttattacgg
     8761 cgcagcgaag aaataacgat cccggtatgt gagccctgct cagtcagctt gtagcgagcc
     8821 agctctttgc cggtttgatc gacaacgcgg cagaaagcgt tttcgacgtc attgaacgac
     8881 tgaccacgga agctgtttac cgtaaacgcc aggtattcaa cgtttgcagg caggcgggag
     8941 aggttgacct taatcacttc atcgtcaccg tcaccctcac cggtcaggtt atcgccactg
     9001 tgcacaacag cgccgcaggt ggatttcagt ttgcggaacc agatggtgtc aattgtttta
     9061 cctgtgctgt ccattaatac acagccagca tcgagatcga ttgagtcgtt gcctccaaac
     9121 aatcctccaa gaagaccttt tttcttaacc ggatcccaac cgagaccgaa atgaagctgg
     9181 cttaatgcag atgattcttt gctgagtgat accgtctggt tcttaactaa tgaaaccatg
     9241 ttttattcct taaaatggta atgaccgaga caatcaaagg tatatttagt aactgacgaa
     9301 gaaaatcata tcatgatgaa gttctctgac aagggaaatt tttgtggttt tctgtaaaaa
     9361 cacaaaaaga aatccttttc agggtgttag tttttctatt tttaatttaa atatcaatgt
     9421 gttataagga atccaaaaaa cactgacgca ctccataacc acaggagtta ttaatcatat
     9481 tatgattgac atgtagctta gacactcata gactcacttc tcaatcataa atttgcatgg
     9541 cgttccggat aattaaaaaa aatgaacaag aaaatttggt ttatggaagg tttatcctcc
     9601 cagcgagata ttattcaggg ggtaaaatca tttgcacaaa aaaataattt tgccattacc
     9661 gtttttgcct cccaccgtaa cgaaagaaat gaaatccttt ccgttgccga ttattctttg
     9721 actgaacctg aagatcctca aaaacgtctt cagtttatcc aggaaaccat tcagacctac
     9781 ggcatccacc atattcatac tggccgtaac agccagtggt ttgaagaaca ccgttcagcc
     9841 attgaaccga ccggtgccac ccttactacc ggtgcaacgg gcgtcgactg gttaactctg
     9901 gctgacgaaa aagttacttt tgctcagttt atggagcaaa agggtctccc ggtcgtacca
     9961 tcctggcggg tgaatacgct ggcagaatta aagacacacc tcgcggcccc gccgttcact
    10021 gacagcccgg tatgcgtgaa gccggtgacg ggtatctatg gcatgggatt ctggcgcttt
    10081 gatgacagtg cttcgcctat ggccgtcttt aatcatcccg aacatcgtct ggtcagtccg
    10141 caacagtata ttgcagcagc atcagctgct gagtcgttta aaccccttgt tttgatgccg
    10201 tacctgccag gcccggaatt ttccgtcgat atcctcgcgg ataagggcga aatactcgca
    10261 gccgtgggac gccgtaagga aggggctatc cagtatctgg taaacgaagg aagcgcctgg
    10321 gaactggcgt gtgactgcgc ccgtgttatg aaggccgacg ggctggtgaa tgttcagacg
    10381 cgaaacgatg tgaatggcaa cccggtgctg cttgaaacca acatgcgtcc gtcagggggg
    10441 gtgggttata cccttcacag cggtgtgaac cttcctgggt tatttgctgc ctttaagctc
    10501 ggtctgatgt ctgaagatat ggtacgccag agcgctaaaa acaccttttc tccggttgcg
    10561 gtgagatcca ttacggatgt aattgcatac ccggaatcac tctctaacct tctgaattaa
    10621 ggcgtattta tgtctgatat cctcgaagat atgatttatc gccgtaccct ctcatgcggt
    10681 acgattcagg taacccgtga ccagggtgaa gtttcgctcg atgacctgtt tgacatcgct
    10741 gaaagacgta acccgaaacg cgcctttctg tttgtcagca aagtactcgg caggcacatt
    10801 cccgtgccac cctcagtcat gcggcaggcc tacagacagc ttgccagcca gttcccctcg
    10861 acactgacag gacccgtact gtttatcggt atggctgaaa ccgccgttgg gcttggggcc
    10921 ggtgtatttg atgaagtgcg ccaccagcat cgtgaatctg tctatctgac ctctacccgt
    10981 cacccggttg atggcacgtt gctttgcgag ttcaaggaag aacacagcca cgccaccgat
    11041 catctgatct atctgccaga tgatgaagag aaaagacgtc gtgttaccaa cgcacgaacg
    11101 ctggttttga ttgatgacga ggcaaccacc ggtaatacct ttattaacct gctttctgcc
    11161 ctgcgtaata ccggcaagct tcaacatatt gaacaggtta tcgccgtaac gctgaccgac
    11221 tggagtggca atgccctgtc ggagcgcacc cccttgcccg tcacttcggt ttcgcttgta
    11281 agcggtcagt ggggatggac cccattacct gatgcacctg tcccggacat gccgaaagtc
    11341 aacgtaactt cacgaggaga atgggacatc caggggaaac agtcatgggg ccgactgggg
    11401 atgctcgcac ctgcggccga tctcggccat gaggtctccg tccacaaggg ggaacgtatt
    11461 ctggttctcg ggaccgggga attcgtctgg gagccgttcc tgcttgctga acggctcgaa
    11521 gctgccggag cacaggcatt ttatggatcg accacccgct cccctgtcgc cgttggttat
    11581 gccattgagt ccgccatttc ctttacggat aactacgggc tgggcatccc caattttgtc
    11641 tataacgtcg cccaccagca gtttgaccgc attcttgtgt gtactgagac acccgcagaa
    11701 agtattgaca cgcagcttct taaagcgctg gctgaggttg cgcccgtcgt ggagattgtt
    11761 acctatgaat aaacccgttg ttctgagcga ccttgacgac acgctctttc agacccgtcg
    11821 taaaatggtg gacgagctgg ccctggagcc attccgtacc ggtgccgttg atcgcaccct
    11881 gaatccacga agctttatga cggaagaaca gtccatgttg gtggactggc tcctggagca
    11941 ggctgaactc atacccgtta ccgcacgggg aactgaagaa atcagccgcg tacggatccc
    12001 tttccactcc tgggcaatca caacgcacgg ggccgtcatt ctcacgcctg aaggcaaacc
    12061 cgacgaggag tggaaggccc atatgctcgg ccagctggct ccctaccagg aaaagctgac
    12121 atcgatgcag cgtctgatca ctgaaatgat ggacgcaaaa ggaatcaatg cctgggcaag
    12181 gctgaacttc gaatacggtg aaacggcggt ttatatggtg atgaaacacc gcgacagcac
    12241 ccgccttgac gagctcaatg ccattgcaga tgagatagaa acggtgtttc cgaccgaggg
    12301 cttctacatc caccgcaaca gtaataacgt ggcctggctt cccaccccgg ttgagaaagg
    12361 gctggcagtc agctggctgc ttgaaaaact tcgggctgaa cgaggagttt tccccgtaat
    12421 tggtttgggc gacagcctga gcgatcatcg ttttatgaaa ctgtgcagct ggtttggcat
    12481 cccgcgtcag agtcagtttg cagatgccat ttcacagcga atttttggag aaaattaaaa
    12541 tgcagccaca tgaaacattt accggctcat accagcccgg tgacgtggaa tttctgctaa
    12601 agccggttgt cattgagatg acgccggttg agaaaaaaga agaactgatt cagtccggga
    12661 agaaacatta ttcggacatg ctcagccagg agccagcgcc tacacaatgg catcttgatt
    12721 tgtttcaccg ggcgctggat cggggcgcag agaggcttgc aaaagaagtc acacagctcg
    12781 ctattgctct ggccgaacgc ttcggtgatg agcccattgt actggccagt cttgtcaggg
    12841 ctggcgttcc gctcggcgtt atgctgcacc aggccctgcg tgacatgggg aaaacctcat
    12901 ggcattacgg tatcagcatt atccgggatc gtggaattga cggtgcagcc ctcgacgtca
    12961 ttgaagagcg gcatggtacc agcggtattg tctttgttga cggatggaca ggtaaaggcg
    13021 caattaccgg agagcttgta cgcgcactga aagatcgccc gggctatcct gagcagccgc
    13081 gactggttgt cctggccgat ccctgtggct gctcctggct tgcagccagt gatgatgact
    13141 ggctcattcc ttttggcatc atgggggcgc cggtatcagg cctgatctcc cggtcagtat
    13201 ggtcctctga aggattacac gggtgcatgg tttgcgagca tctcagtgaa ttcgaatgca
    13261 gccggatgct tgccgatacc gtcgctcatt tccgtaagaa gttaacaccg tcatccctcg
    13321 ctcccctgag ctggaatacg gaatcagccc gggtcttatg gcagacaagt cgcgacgtta
    13381 tcgcgttcct ggccgatgaa tttaaagtgg acagcgtcaa tcgtattaaa cccggtattg
    13441 ctgaagcaac ccgggctgta ttacgtcggg taccggacca tgtatttgtg cgttctattg
    13501 acgacccgga cgttgccttg cttgtagggc ttgctcgtga aaagggaata gttgttacag
    13561 aaatgggggg aaccctcggc cagtatcggg ctgtaaccat tatcaagaag gtactctgat
    13621 gaaaaatcgt ctttctccct ggaatctggg agccacgcta tacatgcctg caacacggga
    13681 agatattgct gatgccgtcc tgcacgggaa aatcccgggc ttgcgctctc tggtgatttg
    13741 cctggaggat gctgtcagtg aagcagacat ccccgtcgcc ctcaaaaatc tggagcacct
    13801 gctgcacgag ctcagtaaca gcatgagtag cctgggtaaa aatgactggc ctctggtgtt
    13861 cattcgcccc cggcatgccg aaatgggcag atggttaaaa gcgcattatg atctttccgc
    13921 tgtcgacggg tttgtgctgc ctaaatttac cctcagttcg ttagctgaat ggtgggatat
    13981 catggtcggg actcacctgt gcatgatgcc aacgctggaa acagaagatg tctttgacgt
    14041 ggttcagatg cgcgagctgg ctacccgtct ggaggaacat ccctgccacg accgcattat
    14101 tgcgcttcga atcggcggca acgatcttat gaacgttgtg tcgcttcgcc gtccccggga
    14161 cctgacgctg tatgacagcc cgatgggcta cgtcatcaaa atgctcgttt cggtcttcgg
    14221 cccgcgtgat tttgccctga ccgcgcctgt atgtgagcat attgacgatc atgccgttat
    14281 ggccagagag ctggctcttg atatggcaca cgggcttgtt gggaaaacgg ccattcaccc
    14341 gggtcagata gaggtcattc aaaacgcgct gatggtcact cagggtgagc attctgacgc
    14401 tctgcggatc ctgaactcta cccaggccgt gtttaagtcg cagggagcaa tgtgtgaacc
    14461 cgccacacat cgccgctggg cggctggcat tctggacagg gctcgttttt atgggttaca
    14521 gaacgagcaa agcgctgatg gaatcagatt acttaccgtg acccagcatc attaaggaaa
    14581 tcataatggg gttcaggttc agaaagtcga tcaacattat tcctggcgtt cgcctcaacc
    14641 tgagtaacgg tgcaccgagc ctgagtgtcg ggccgagagg tgcttccgtt tcttttggta
    14701 gccgggggac ctatgccaat ctgggcttgc ccggtaccgg gctgagttac cgtacccggc
    14761 ttgaccgggc cgcgcgttcc agaggtgaaa accggacggc aaccgacccg gggctcagac
    14821 aggcgcttga gcagaaagcc gctgaactca tgtcagcggt aaccgcaatc cgtaatattc
    14881 acgagctgac gccggatcca aaaacaggca tcagctgggc agagctggaa gcagtatacc
    14941 tgcataacag aacgtcgcct tttcaggttc cggcaccggt gcgtccagaa aagccagact
    15001 accttgtatt gccggaaaag cctgccgaga gcgaaggcat tagttttttg ggtaaatggt
    15061 ttgaatcgga atcagctaaa gctgagcgcc acgccgaaaa tcttcgccgg tggcagcagg
    15121 agctgattga tgtggagcgt gagaataccc ttcgacagca ccggtaccag caacaacgga
    15181 cggcctgggc cgaacagtat gcaaactgga agtttgaagc tgaagaacat gaaaaacggc
    15241 tcgccacggc tcaggcagat gcccggcagc agttccggac agacgccgcg tttttcgaat
    15301 catacctggc gggtgtgctg gcagaaactg aatggccgcg tgaaacgctt gttgcatttg
    15361 aagtaaagcc ggagctatca gcagtcctgc tggacgttga tttagctgag attgaagatt
    15421 tccctgataa gatttacggc gttaatgccc ggggaacgga gctgacggaa aaagccatga
    15481 cgcaaaaagc cgtacgcgaa aactatgccc accacgtcca tggctgcttg ttccgcctgg
    15541 tcggtatcgt tttacatacg ctacctttcg acaacgtgat tgtgtcaggc tttacgcaac
    15601 gggtcagtaa gcggaccggc tatctggagg atgagtatat cctgtcctgc aaatgcactc
    15661 gcagccagat gtcgtcagta aattttgcag gcataaaaca cattgatccg gttgaagcgt
    15721 taggcgatca accggttatt cgaaagatga gcagtacctt catttttcag cctattgaac
    15781 cactaaccct ttaagcaggc taaatcttac gatgcaatta aataccagac aggcccggat
    15841 ttttaagctt gccaacttac tgggcaccgg caagcccgtt tccgctgccg acattataac
    15901 cagtctggaa tgctctgagc cgacattaac ccgggccctc aaagagctac gcgagtcata
    15961 ttctgcggag atcaaataca gcaaggcagg tcattcctat catctcgtta atccgggcca
    16021 gttggataaa aagaccctcc gccggatgaa tgaagcgctc gcacagaatg ctgaactgaa
    16081 aaccggtgag tccacaggga aggtcgtact cgataaggat aaaaaaacag ccgtgtcatt
    16141 atcgctacgc atgcggattc taagaaaaat tgaccgtctg gctgcgctga gtgactcgac
    16201 ccgaagtgaa gcggtagaga agctggctct gcactccgtt gatgaactga taaaagaata
    16261 cagcgctaaa aagtcctgac gctaaatctc agaccggggt gaaaactccg gttttgtcat
    16321 atttcacctc tgtattggcg tgatgcaaaa tggcatctga atcgatcatg gtttggcatc
    16381 catttccgat cactgtttgg catcacaatc gatcacgatt tggcatgctt ccgctcattg
    16441 attggcatcc tggtgtcgtt acggggaagt catcttcaca gggatcttca gtcagttagc
    16501 attaccgaca tccattcacc aacttaccgg agataatcaa tgtcaaaacc ccgatggacg
    16561 cttgatcaga aaaaacatca cgttgccgca tggcgcgcca gcggccttac ccgtgaacag
    16621 tactgtgaac tttacgacat tccgtttaaa tccctgcgcc agtggccgca ggatgtcgca
    16681 aaagccgaaa aacgagccag agcaccagaa attattcctg tttcagtatc tggctcttcc
    16741 ggaatgactg atggcagacc attgtcggat gagcctgtca ctctcttcct gcccggtggt
    16801 atccggatgt gctgccagcc ttctcagtta accgatgttt tccgggctct tcgccatgct
    16861 gacgcctgat aacgtattcc tggtcgtaaa acccgtcgat atgcgccggg gtatcgacac
    16921 actgacacag tacgtccaga acgaacttaa cgctgcatgg catgacggtg ctgcttttgt
    16981 cttcaccaat aaagtccgct cccgcataaa agttctccgg tgggacaaac atggcgtatg
    17041 gctgtgtacc cgacgactgc accggggaag cttccgctgg ccccggaaag gtgatgccac
    17101 atggcatctc acacaggatg agtttcactg gctggtcttt ggggttgact ggcaacaggt
    17161 gaaagggcat gacctggcaa aatgggtata ccagtaagaa cccgaagatg ctcagagcaa
    17221 cgcaataact caatgaaata taatgaatat ccatgcgtga tgtcatataa tccaccgcat
    17281 ggatatctcg cttctcagta ccaccagtga ccctgaacag cttcgcgctc tggcaattgc
    17341 catggtgcaa aaagtcatgg ctgagaatgc agaacttcag aaccgtatcc gcatcctgga
    17401 agagcagatg aagctcgcac gccagcagcg tttcggtaaa aaatgcgaat cgctggccgg
    17461 gatgcagcgt tccctgttcg aagaggatgt tgacgcggat atcgcagaaa tctcggcgca
    17521 tctggacaaa ctgcttcctc agaccgggga cgaagaaaaa acaacaacac gtcctgttcg
    17581 taagcccttg ccttcacctc ttccccgtgc tgaaaaggtt atcccgccag ctgaggaacg
    17641 ttgtcctgac tgtgatgccc ccctgcactt cattcgcgat gaagtcagcg agaagctgga
    17701 atatattccg gcacaggtcg tggtcaaccg ctatatccgc ccgcagtaca gctgtccctg
    17761 ctgtgagaaa gtgttcagcg gaaaaatgcc ggcacatatc cttccgaaaa gtgctgttga
    17821 gccatcggtc atcgcacagg ttgtcatcag caaatatacc gaccatctgc ccctgtatcg
    17881 ccagcagcac attttctccc gaatgggggt ggagctcccc gtcagtacaa tggcggatat
    17941 ggtcggtgtt gctggcgctg cactggcccc gctggcaaaa ttgttacgcc acgagcttct
    18001 gacacgggat gttattcatg cagatgaaac gtccctgagg ctccttgata cccggaaagg
    18061 cggtaaatcc tgctccggct ggctgtgggc ttacgtcagc ggagaaagag taagcgtcaa
    18121 cggagcaccg tattgacgct tatttattgg tgagtactac gttccatggc aggagttcat
    18181 caactcggtt ggaaggccat tctggcagta cgctcaggat atggcgcaga tacgcttccg
    18241 gatcgatacc gttcagacgg caggtgccga tcagcccgta caacagtgct ccacgctcgc
    18301 cgccgtgatc gctgccaaag aacataaagt ttttctttcc gagacagact gcacgaagcg
    18361 ctctttccgc agcattatta tccgcctccg ccagaccgtc atcactgtaa taacagaggg
    18421 catcccactg attcagtaca tagctgcacg cttcgcccag tctggatttt ttcgacagcg
    18481 taccattctt ctccaccatc cattcatgca gtgacgtcag taacactttg cttcgctgct
    18541 gcctgacggc aagacgctct gactccggta atccccgtat ttcatcctcg atggcgtaca
    18601 gttcactgat tcgcttcagg gcttcttctg ccgtcgcact tttgctgctg atgtatacat
    18661 cgtggatttt tcgccgggca tgggcccagc acgcaacttc tgtcagtgca ccaccttcac
    18721 gttctgcact gaacagccga tcgtaaccgc tgaacgcatc cgcctgcagg ataccccgga
    18781 agggacgaag gtgttgtacc ggatgttttc cctgcctgtc tggtgagtag gcgaaccaga
    18841 ccgccggtgg ctctgatgag cccgcattcc ggtcatcccg gacatacgtc cagatgcgtc
    18901 ctgtttttgc cttttttctg cccggtgcca gcacttttac tggtgtgtcg tcagtgtgaa
    18961 ccttgcgggt gttcatcacg taacggtaca gggtatcatt cagcagagtc attaactgac
    19021 aacatgcgtc aacccagttg gagagtaatg cccggctcag ttcgatgccc tggcgggcaa
    19081 agatttcact ctgacgatac agtggcaggt gttcgcagta ttttcccgtt aacacgcggg
    19141 caagtaatcc ggagcccgcg atgccgcgct ctatcgggcg ggacggcgct ggcgcttcaa
    19201 ctatacagtc acattttgta caggcttttt ttacccgaac agtgcggatc actttcaggg
    19261 cgctactcac cagttccagc tgctcagcac tgacttcccc cagataatcc agctcactgc
    19321 cacactccgg gcaacaactt tcttcaggct ccagacggtg tatttcacgg ggaagatgtg
    19381 ccggtaacgg acgacgatga cgtgactgtc gcaactggcg gggaacctgc gggtcatcct
    19441 cacgcccact gtaacgatcg ctttcctgtt cgcgttgttt cagttgggcc tcagcctgtt
    19501 caacctcacg ctgcagtttt tcagaacggg taccgaacaa catccggcgc agtttttcta
    19561 tctgggccct cagatgttct atttcccgct cctcctcttc gatcttttcc tcggcacgtg
    19621 ccagtgcaga gcgcaggaag gcctccgtct cttcaaccag actcagttgc tgatctttct
    19681 gacggagggc ttcagcctgc tcagagagca gcctttccag ctcagtgata cgaatgaggt
    19741 atttccgact catgaccgtt tttataatcc ggccatgaca tttttacaac attgtcagtg
    19801 cattaaggcg ggatgttttg ggctgacgcc agtccagttt atcgaggagc attgccagct
    19861 gcgagcgggt aatggatacc ttaccgtcac gcaccgcagg ccagataaac tggccttcct
    19921 ccagacgttt ggtgaacagg cacagaccat cagcatcagc ccacagaatt ttaatcgtgt
    19981 caccccgtcg gccgcgaaag ataaacaggt gaccggagaa ggggttctca tccagcacat
    20041 gttgtacctg ttctcccaga ccgttgaagg atttacgcat atcagtaacg ccggcaacca
    20101 gccagattcg agtgcctgat gggagcgaga tcatcgtccc ctcccggtca gttcacggat
    20161 caacaccgtg agcagctctg gtgaaggatt ttccagcgtc atgttaccgt ggcggaactc
    20221 aactttacag gaactggcac tgactgtgct ttgtgaagga gtggataaaa gcggagtaag
    20281 agccgccata ggctctttct gctcatcagg cgttatctca acaggtaata attcaacgcc
    20341 agcgccagaa gaggttgtta ccggaagacg ccgcgatata cgcctttcgt tctgccagag
    20401 cctgagccat ttgaacagga ggttatcatt gatatcgtgt tcccgggcaa tacgggcaac
    20461 agaggctcct ggttgtgaag ccagtttaac catttgaagt ttaaactcat ttgaaaatga
    20521 tctgcggggt tctgaggata atattttctg ttccataaca ggtgtccact agttgaaaag
    20581 gtgggcacct acgttaccaa tactggctta atggctacat acggcggtca gtttacgctt
    20641 acgagaaaga agcggtccgc cggtcgtctg ttttgactcg cagaccggac gggccctcag
    20701 atatcctgag gcctggctgc agggctggtg tggcggtacg ctggtgagtg acggatacag
    20761 cgtgtacaaa tctctggcgg ataatgaccc cggtattaca tctgcctgtt gctggagcca
    20821 cgcaaggcgc ggtttcgcaa atctgtataa ggccagcagg gagccccgtg cagccatggc
    20881 gctgagaaaa atcgcgggtc tttatcgcat tgagaagttc atccgtgaac gtcctgtgga
    20941 aaaaatacgg caatggcgac aacggtattc ccggccgata gtgaatgacc tgtttgcctg
    21001 gcctgaagag caggagccgt gctgtccgcc cgacggacca ctaaacaaag cgattaatta
    21061 tatcctgaac cggcgcgatg aactgagctg cttcctgagt gacggagccg tgcccctgga
    21121 caacaatatc tgcgagcgtg ctatccgccc ggtggtcatg gggcggaaag catggttatt
    21181 cgccggttca ctgatggccg ggaaccgcgc ggcacagata atgagccttc tggaaacggc
    21241 aaaacgtaat ggtctggagc cgcatgcctg gctgacagac gttctgacgc gtctaccgga
    21301 gtggccggaa gaccgactgg aagagttgct gccccttgag ggatttacct tctccgggtg
    21361 agtgatacct gccatcaggt gttcgtgcag cgggccataa cccgcagtcg ggagttgaac
    21421 gcctgacgac aggaaatgag ccagagcagc gaccacgcgg gctgacagtg agtcagaacc
    21481 tgaatcagcc cggagccatt cccggggctg tacttcatca cctgccatca gtacccgtgt
    21541 cagtacttcg cgacctgacg caattacccg actcagttct tcagaacccg cctccgatac
    21601 cagcctggcg gtcgcccctg tgcaacaaaa taaatcaacc atcgtcagac ggtgatcaat
    21661 cgtatgcgct gccagtggct cgcctgaaaa atgcgtcaat atgagttcgc cggaacaata
    21721 cctcagttct tcagaacccg cctccgatac cagcctggcg gtcgccccgg cgcaacagaa
    21781 taaatcaacc atcgtcagac tgcgatcatt cgtatgcatc gctgatgatg cgcccgaaaa
    21841 acgcgtcaat atgagttcgc cggaatatta ctgacgacac ggtttgtagt tacgttcctt
    21901 cggtgagtga gtacctgtgc gtcagggtaa cgcaatcagt tcaggtttag cggggtgtca
    21961 agtcggtttg gctaaccgac cgcagaatta actgtacctt taaaggtcgc ccccgatata
    22021 tctgcctgtc agacatatcg ccgattcggt atctcactac ataaaactcc ttaaaaaaca
    22081 atgcgattct gttggtacga agctttcagt aagtcagtcc ggagaagcgc cagtacgata
    22141 cagaatgagg ttgaaaaaaa cgttcacgtc tggtcacttc ttcaaatgtt aaaaaacatc
    22201 tcgtaaagag ccagtttatg cgggttcgtt gagtagacgt gcgagcgtca ctttgtcagc
    22261 tttaacgctg acgaaagtga cgcccgcaca gcttcaaact ccacatgcac acgcaagcga
    22321 ccaaatttca aatggtctta tgccgtgcgc acagtcataa gaagcgcatg cggaaagtca
    22381 gaaaatatct taagacgcag attttaaagg cgtttttatg ccagtctgcc gggaatacac
    22441 agtagtccgg ttgtccggaa gcaccacggt aatccgaagg aaaaatcaac gtacctcaat
    22501 caccgtagtg atatccgttc ccagaggcta aacggatgta ccaccataaa ggcatcctct
    22561 ttcaggctac gaggatattg caatcacccg aaggcgtttc tctcagattg ctgaaacatt
    22621 ggctggcagt cacccgaagg cgtccctctc agactgctgg aacattggct ggtcgcaaaa
    22681 gcgacagtta atgcaaatac aggttaacaa aaagggaaat ctgccgtcaa cccaaccata
    22741 tgaacatgtt gatttcatta tgaatgtgat gttctaatat aaattaatca gtaacttcat
    22801 aaaaagaata taagaaacag ataatgcatg tatttacaca catatcagca caatgataga
    22861 ttaccacccc agaaacacga taaaggtata attatggaat tttccgttat ttttttctgg
    22921 gttatcactg gtcttattac catcttattc ttaaaatcaa taataaccga tctaataaaa
    22981 aacccaaaca aaacagacat agcgttatat atattggcat ttttatcagc cggatataca
    23041 ttccagtata tttacaatga caatatattt tcagaactat ggggagttgg cctgtttgtt
    23101 actctgatag tactgaatat agtatttaat ctctttttta gactcttatt cattctctcg
    23161 aaaatgatat taatgtcacg acatataaga aagatgcaaa aaaaactaaa acagacgtct
    23221 gtgcctgatt ttatttatta ttcaatagaa aaacacaaca aatatcccat atatgtatta
    23281 cacatgccat caaacaatat aattgaaata ggctacaata taataaacac cgatctggtc
    23341 attggcgaaa aaattcattt caggacacta agcaatcgtt ctttatattt taacctcacc
    23401 caccccccat taatcgccac aataaaagat gatgtgtttt gcactcttca tgattactat
    23461 aaccacaata atgaaacgaa atccactatt gacaattaca tatcaaaaat aaaggataat
    23521 cataataccc catggttatt aaacaacgaa aatgttcagg aataaaaaaa agagacaatt
    23581 cccgagataa aacaaaagag cataaacata tgaaccggaa attctatttc aaaaatatcc
    23641 tgtggggaat gtgccccatt ataatatcca tattgcatct gatatatgac acaccaacag
    23701 aagcatcgat aaaagttgac ttatttctga tactcagtgg cctggccctt ccttgttcaa
    23761 tatacctgac aaaaacaatc acactaaaat acacatccat atcattctgg aaaaaacctc
    23821 ttttctggtc ttatggtata tatcattttc atcttgttta tataatgata tgtatagttt
    23881 tagcaatacc ttctgcaatt atcgttttat tctttcactt ttatcaaaac atcaccagaa
    23941 actggaagtg acaaccgcaa taatgaacat aaaaaggcaa atcagaatgg cagtaaatca
    24001 ataaagtacc atttcatatg aatcatcaga aaaacggtgg acgcgacatc cagcacgagg
    24061 caaaacacag caacaaaata agccggatat cgcgccctgt acttttctaa ctcaactccc
    24121 ggcattgtgc aagttttaat cgcaggtgga ttcagagcca cgcccccatt gattcagcct
    24181 gaagacacgt atatccttgg agaaagcagg aatctcattg ctgtgaaatt ccttacagaa
    24241 tcttcagccc cattttatgg ggcttttttt ggtgccccca cgctgttacc acactttcgg
    24301 gatgcgaata aaaaagccca cagtttttac actgcggact tttgtttttt ctttgtgatt
    24361 tgtgtgcaag ggtgcgactt tcgaccgaac ctttggcaac cggatagcgg ggactgttgt
    24421 ccccgctgcg gtttcttacg gtttacactg caagaacgcc gcaatttccg accacatgaa
    24481 gcttaccctg aattcacacg gtgatgcgtg taacacccag acggtcagca acatggtcag
    24541 gcataacatg cccacaatga tcgttttttg cggcatagca cttgctcctt ttacagagag
    24601 gcgctaacct tccacttgct gaaggtagat ggtcagggcc tcgggttaac gtttaagttg
    24661 actcggggcc tttccacatc cggccttcag gtaatccttc cagccgtcag ccgaaaggca
    24721 cccgcgcgta atgtactggt tttcagagag gtccgcaatc ttccggtgac gagatcatct
    24781 gcacacgccc cggtgggacg ggctaacggt atattcaggt gcaggatctc tctgtctggt
    24841 gcgtttttaa attgtcaaac gcaccctggc ggcaaaatca gccacctttt ttcgcatcaa
    24901 caaaacgccc aaaacgctgg gtaaagcgtg caacattacc ttctgaagcg acagttctca
    24961 gtttacctgt ataaaacgga tgcgacttag aagaaacgtc aattgtaaca tatgggtaag
    25021 ttacaccatc cagttcaatt acccggtcag ttttgatcgt cgaaccgatc ctgaagtact
    25081 catcaatact ggtatcgtga aatactactg tacggtattc cggatggata ttcggtttca
    25141 caacagtttt ccccctttaa gtgaaaattc cgcataaagg tacatattca gacggtcaga
    25201 ttcaactgtc tgttcttata acctcactct ctttcagtat tacaatatgt tatataatta
    25261 taaactggac ttcccgaaaa aatccattaa ataagaagcg agccgttgcg gaggcttgct
    25321 ctctccttta acctcatgtg caatatccgt aacgcgctca cataaatcat catctgataa
    25381 tataacaaca cccattattt tattctgacc taaaaacttc ttatgaaata aacgtttacc
    25441 cgacgtatct gatcccgctg tttttacctg tatcctttaa catacttgag aagcccgctg
    25501 aaaaaatata ccgtaaaggc ggtttctctg tcttccataa acctgtcgga taagaagggc
    25561 attaaatgaa aaccaaaaaa gttatgatga ttgcactggt cagctctgcg ctggctcttt
    25621 caggatgtgg agccatgggc acagcgatca aaaaacgaaa tctggaagta aaaacccaga
    25681 tgagtgaaac ctgaggtagc ctgagtttaa cggacactcc ttcctgaaat agaatggcat
    25741 cagaaggagc taataatgag cagaaaaacc caacgttact ctaaagagtt caaagccgaa
    25801 gctgtcagaa cggttcttga aaatcaactt tcgatcagtg aaggcgcttc ccgattatct
    25861 cttcctgaag gcactttagg acaatgggtt accgccgcca gaaaagggct cggtactcct
    25921 ggttcccgca cggtggctga actggaatct gaaattctgc aactgcgtaa ggcgttaaat
    25981 gaagctcgcc ttgagcgaga tatattaaaa aaagcagctg tagattcaat ctgtcaatgc
    26041 aacacccctt tcaattatct ctttcactgc ccgcgacggt atcggttatc agtttgacct
    26101 gctggctaac ggtgaaaccg tactgcgtga tgcatccggc gaaaaacgca tcaagggtga
    26161 aaaagacggt cgtatgctca tgaatgttgg cctgaatgcg gaaattcgcg acaacatccg
    26221 cttcggtctg gagtttgaaa aatcggcatc tggtaagtac aacgtggata acgcggtgaa
    26281 cgccaacttc cgttattcct tctgataaaa acaaggggcc gcctgccggc cctgatacag
    26341 aggatgttgt gattacttac ccagcatgcc ctgctcttcg ataaaggcga tgatgttttg
    26401 cagaccttca ccacttttca ggttagtgaa cccccatggg cgttctccgc gcatacgtaa
    26461 agtatcacgc tccattacct cgagggaggc gcccacgtat ggtgcaaggt cagttttatt
    26521 gatcacgagg aagtcggatt tggtgatccc cggaccgcct ttacgcggga tcttttctcc
    26581 ttctgccaca tctatgacat atatggtaag gtccgccagt tcggggctga aggtcgcact
    26641 caggttgtcg ccgccgcttt ccacaaagat cagctcgaga ttaccaaatt tctcactcag
    26701 tgcttccacg gctgccagat tcattgatgc gtcttcacga atggctgtat gagggcagcc
    26761 ccccgtttcg acaccgacaa tgcgttccgc ttccagtgct ccggcctcag tgagaatgcg
    26821 ttgatcttct ctggtgtaga tgtcgttggt gactacggct aattgccagg tgtcacgcat
    26881 ggatttgcag agggcttcca gaagcgcggt tttacctgag ccgaccggac cgccgacgcc
    26941 gacacgcagc ggttgcttga ttatgttcat tttattttct cctaagaacg gaataatcgg
    27001 gaatactggg tttcatgtcg cgcggaagcg atagatgcaa gtggcgttgc tgaaccaatt
    27061 gcgtgatccg gggttgcaag ggcactgtcc atgttcgccg catagcgggc acacaggcgc
    27121 ataattaact gctgggcagc cagctggccg tagggtacca gcctgacgcc cgccatcact
    27181 gcgctttcaa tccagctgta gccaaggctc agggccaggt ctgaaacgga tatttgccag
    27241 cgtacgccga gccatgccat accggttaac tggctttgct ggcatagttt gcgccattcg
    27301 gccgggcagt cgggctgcca gtcgaccaga agtctggtga aggctgcacc gcggttacgt
    27361 tcttcatcgc gtagctcacg ggtttccctg caggccagca gataagcggt ccagcgcccg
    27421 gcactgtcag gatcgcccgc ttcacaggcc cggtatagcc gggcaagcag tggcaggtcg
    27481 acggcaaaaa aactctgttc catctgcaac tgctgccagt gttcaaaggc ggcgctatct
    27541 tctacccagc ctgcttccac tgcccactcc agtccctggg accagctgta gcctccaaca
    27601 gggaggctgt tactggcaag ctgcatcagc cgcagacgtt tttccggagt cggcattcag
    27661 tggctgtgct cctgatcgtg attatgggcg tgcgacttgc tggcgtaagc gccggcttcc
    27721 ggctcaaacg gaagatgtgc aaaatcaaca tccagcccaa actggcgcag catatcatcg
    27781 aggacgtgat cgtgatggta gcgcagctct ccgggcatta tttgcagcgg aacgtggcga
    27841 ttgccgagat gccagcaggc tttcgcgagc ataaaaggat ctgcgcagcg cactacagaa
    27901 acggcttcat cagcggcgat caccttaatg aattcatcac cgttttcgtt actgagaata
    27961 tcaccatcgc gcagcagcag accgcgtggc agcaacagtc cagcctgacg tccatcgtta
    28021 agggtgactt taatacggct ttttacccgc atgtcaacag ggagggtaac gctggcagtt
    28081 gtctgagccg gggtctccac ccgccgggtc aaataaagca tttgtgctcc ttaaaacagg
    28141 aaataacgtt gcgccattgg cagaatgtcc gccggttcac tggttatcag ttcgccgttg
    28201 atacgcactt cgtaggtttg tgaatccacg gttatatcag gcaacaggct gttgtggcgc
    28261 atatcggctt tttgtactgt gcggcagcct tttaccacag cagttgtgct gtgcaggtta
    28321 agctgttcag cgacgccatt tgctgctgct gcctgcgaca ggaaagtcac acgacagcgg
    28381 tgacgggcac tgcccaatgc agcgaacatt gggcgatagt gcaccggctg cggtgtgggg
    28441 atagagccgt tgatatcacc catcggcgcc atggctatca ttccgccttt gacgatagtc
    28501 gccggtttta cgccaaagaa cgccggggac cagagcacca gatccgccag ttttcccact
    28561 tcaatcgagc cgacttcatg agcaataccg tgggttaatg ccggattaat ggtgtattta
    28621 gcgatatagc gcttcacgcg gacgttgtcg ttatcaccac tttcttccgg taacgggccg
    28681 cgctgaactt tcatccggtg tgccacctgc caggttcgta acactacttc tccgacgcgt
    28741 cccatggcct gcgaatcgga cgaggtgagg gagaacgcgc caaggtcgtg caggacgtct
    28801 tccgcggcaa tggtttcctg gcgaatgcgc gattcggcaa aggctacgtc ctcggcgata
    28861 tccgggtcaa gatgatggca aaccatcagc atgtccagat gctcatcaat agtgttgacg
    28921 gtatagggta gcgtcggatt ggttgaggaa ggcagaatat tggggtgcgc gcaggcggtg
    28981 ataatatccg gagcatgacc accacctgca ccttctgtat ggaaggtatg gatagtgcgc
    29041 ccgccgatgg cagtcagagt atcctcaacg aatcctgatt cgttaagcgt atcactgtgt
    29101 agcgcaacct gaacgtccat ttcgtcggct acagtcagtg cgcagttgat taccgcaggt
    29161 gtagctcccc agtcttcgtg aattttgagg ccgataaccc cggccgcgac ctgctcacga
    29221 agcgcatccg gattggagca attgccttta cccagcaacc cgatattgac cggcaggctg
    29281 tctgcagcct gcagcatttg ataaatgtac catggtcctg gggtacaggt tgtggcgtta
    29341 gaacccgctg tcgggccagt accgccaccg atcatggtgg taatgccgga tgtcagcgct
    29401 tcttcagcct gctgtgggca gatccagtga atatgcgtat cgacgccacc tgcggtaacg
    29461 atcctgcctt ctgcggcaat aatttccgtg gatacgccga ttgggatagt gacgttgggt
    29521 tgtatatcag gattaccggc tttgccgata gcaaatatcc ttccatcttt gacgccgata
    29581 tcggctttaa cgatccccca gtaatcgatg atcagggcat tggtcagcac cagatcagcg
    29641 cagccggcgg agagcatttg cccctgtccc ataccgtcgc ggattacttt accgccgccg
    29701 aatttgacct cttcgccgta ggtagttaaa tcatcttcga cctcgatcca cagctcagtg
    29761 tctgccagac gaattttatc accggtggta gggccgaaca tgtcagcata ggcctggcgt
    29821 gaaatattac tcatcatttg cctccagctc acccattata tctccgcgga aaccgtgcac
    29881 gatacgggca ccgctcagcg ccaccagttc aacctcacgc ttttggcctg gttcgaagcg
    29941 cactgcagtt ccggcggcga tattgagacg atagcctctt gccttctggc gatcgaactt
    30001 cagtgccggg ttaacctccg cgaagtgata atgggaaccg acctgaattg gtcggtctcc
    30061 gtgattttca acgataatac tgcaggtagc ccggccgatg ttaagctcta tatagccagg
    30121 tttaacctta tattctccag ggatcatctg ttacctcaga ttatcggatt atggacggta
    30181 accagcttgg agccgtccgg aaaggtggct tcgacctgga tatcggggat catttctggt
    30241 atgccttcca tgacctgctc gcgactcagg acatgccgtc cttcttccat cagcgcagcg
    30301 acgcttttgc cgtcgcgagc gccctccatt ataaaagcgc taatcagggc cacggattcg
    30361 ggatagttaa gtttcaggcc gcgggccaga cgacgctctg ccagcagtgc agcggtaaac
    30421 agtaatagtt tgtctttttc tcttggagtc agttccatag cgttctctta agtcagccag
    30481 atccggggaa gtaccggtga tttgtcagtc agttgcggcc gcataaattg ccagatatca
    30541 cgcattacct actggcaacg cagattatca tcacttaaaa agcgaactgt cagtagacca
    30601 tcggtgagcg tggctccggc gtagttttcc agtggtgtga gtttttcgcg tatgtcttcc
    30661 agttgcgttt cattccccag gtagaacagc atcgttccca cccatggttt ccgggcaacg
    30721 caggtaagtt ctccattggc cacgtgcaga cgctcaatca acagcggaga accgtcgacc
    30781 catatctcca gacggttagc gagtgttccg tggctgaagg tttctccgat gaccggacgt
    30841 ccaaggcaca gaagatccca ggccagcagt gtactggagg acgacagatg aaacacggta
    30901 tgcaacgtgg cgtgagcgcc gggaaaaaaa atcgaatcct gtggcaacca ctccagcgtt
    30961 gcgttattgc acagcgtcag gttttgctga aggtgcgcct gcgcgccgct gctgcggtaa
    31021 aatttactgg caccaggcat ggtgatcagc gtatggcaat cagagtcaat tattgcgttg
    31081 atgatgagtt cgtcaccgcc gacgatacca ccgggtggat gaagcagaca gaggtggcag
    31141 gtttcttctt cgggataaaa aggacgctga acagtgagca gaccgacatg atgacgggtg
    31201 gtgagcgtgg ttttcccgtg cagacgctga aaacggagat ccagcatggc ctgccagcct
    31261 tttttatttt gtggtaacgc ggtactgagc aatcttacgc ccctgataaa taaagataaa
    31321 acgacagtaa agagccggat ttccttgtta tataaaagat gatgcctttc cagtgagttt
    31381 tcgtctgaat aatacgaatg tttgttttcg tcatgaagcg ttccggctcc cggggctata
    31441 catcgccaga aaagcaatgc atattgtcgg gattatccag agaatcaggc agaaagatcc
    31501 gacaatgggc tgtgattatg ccatctggct cagaatgata tcaaggtgtt atggggataa
    31561 cttgctgata caatagcagt atgacagatt cattaatgcg gaaactgctc actgaaacag
    31621 agtgttcatg actgagcact ggtcgggtca tgcagatgat aatttcccct tttcttgggg
    31681 aagcgacagt attttcatgc atgcctgtct tcagtcatac ggggagagca tctgtgatac
    31741 agtccataca tttgttgcgg acgtcgctcc tgagtcactg actggtctgc aaacagttta
    31801 tctgtacacg gcagggcttc tgtgtgatat cgtttttcat atcgaggggg ggcctccaga
    31861 gatgaatgga ataataatat ttcctgatga tataatacct tagagaattt ctgtgtatgc
    31921 tattgtgaac atatttttta ttaaaaagat gttagtggtg ttgtaaaata aaagaatcag
    31981 taactataaa cgcctttatt gacataaagt tttgat
//
LOCUS       DABSYT020000049        31127 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.049, whole genome shotgun sequence.
ACCESSION   DABSYT020000049 DABSYT020000000
VERSION     DABSYT020000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.049"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..546
                     /locus_tag="ID725_RS13375"
                     /old_locus_tag="pgaptmp_002675"
     CDS             <1..546
                     /locus_tag="ID725_RS13375"
                     /old_locus_tag="pgaptmp_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGV7851244.1"
                     /translation="PIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCT
                     GVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRTVGAGVVAKVLS"
     gene            776..1159
                     /gene="secE"
                     /locus_tag="ID725_RS13380"
                     /old_locus_tag="pgaptmp_002676"
     CDS             776..1159
                     /gene="secE"
                     /locus_tag="ID725_RS13380"
                     /old_locus_tag="pgaptmp_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGV7851245.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1161..1706
                     /gene="nusG"
                     /locus_tag="ID725_RS13385"
                     /old_locus_tag="pgaptmp_002677"
     CDS             1161..1706
                     /gene="nusG"
                     /locus_tag="ID725_RS13385"
                     /old_locus_tag="pgaptmp_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGV7851246.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1865..2293
                     /gene="rplK"
                     /locus_tag="ID725_RS13390"
                     /old_locus_tag="pgaptmp_002678"
     CDS             1865..2293
                     /gene="rplK"
                     /locus_tag="ID725_RS13390"
                     /old_locus_tag="pgaptmp_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGV7851247.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2297..3001
                     /gene="rplA"
                     /locus_tag="ID725_RS13395"
                     /old_locus_tag="pgaptmp_002679"
     CDS             2297..3001
                     /gene="rplA"
                     /locus_tag="ID725_RS13395"
                     /old_locus_tag="pgaptmp_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGV7851248.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3293..3790
                     /gene="rplJ"
                     /locus_tag="ID725_RS13400"
                     /old_locus_tag="pgaptmp_002680"
     CDS             3293..3790
                     /gene="rplJ"
                     /locus_tag="ID725_RS13400"
                     /old_locus_tag="pgaptmp_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGV7851249.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3857..4222
                     /gene="rplL"
                     /locus_tag="ID725_RS13405"
                     /old_locus_tag="pgaptmp_002681"
     CDS             3857..4222
                     /gene="rplL"
                     /locus_tag="ID725_RS13405"
                     /old_locus_tag="pgaptmp_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGV7851250.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4542..8570
                     /gene="rpoB"
                     /locus_tag="ID725_RS13410"
                     /old_locus_tag="pgaptmp_002682"
     CDS             4542..8570
                     /gene="rpoB"
                     /locus_tag="ID725_RS13410"
                     /old_locus_tag="pgaptmp_002682"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGV7851251.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8647..12870
                     /gene="rpoC"
                     /locus_tag="ID725_RS13415"
                     /old_locus_tag="pgaptmp_002683"
     CDS             8647..12870
                     /gene="rpoC"
                     /locus_tag="ID725_RS13415"
                     /old_locus_tag="pgaptmp_002683"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGV7851252.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13083..13526
                     /locus_tag="ID725_RS13420"
                     /old_locus_tag="pgaptmp_002684"
     CDS             13083..13526
                     /locus_tag="ID725_RS13420"
                     /old_locus_tag="pgaptmp_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HGV7851253.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(13961..15094)
                     /gene="thiH"
                     /locus_tag="ID725_RS13425"
                     /old_locus_tag="pgaptmp_002685"
     CDS             complement(13961..15094)
                     /gene="thiH"
                     /locus_tag="ID725_RS13425"
                     /old_locus_tag="pgaptmp_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGV7851254.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15091..15861)
                     /gene="thiG"
                     /locus_tag="ID725_RS13430"
                     /old_locus_tag="pgaptmp_002686"
     CDS             complement(15091..15861)
                     /gene="thiG"
                     /locus_tag="ID725_RS13430"
                     /old_locus_tag="pgaptmp_002686"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGV7851255.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15863..16063)
                     /gene="thiS"
                     /locus_tag="ID725_RS13435"
                     /old_locus_tag="pgaptmp_002687"
     CDS             complement(15863..16063)
                     /gene="thiS"
                     /locus_tag="ID725_RS13435"
                     /old_locus_tag="pgaptmp_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGV7851256.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16047..16802)
                     /gene="thiF"
                     /locus_tag="ID725_RS13440"
                     /old_locus_tag="pgaptmp_002688"
     CDS             complement(16047..16802)
                     /gene="thiF"
                     /locus_tag="ID725_RS13440"
                     /old_locus_tag="pgaptmp_002688"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGV7851257.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16795..17430)
                     /gene="thiE"
                     /locus_tag="ID725_RS13445"
                     /old_locus_tag="pgaptmp_002689"
     CDS             complement(16795..17430)
                     /gene="thiE"
                     /locus_tag="ID725_RS13445"
                     /old_locus_tag="pgaptmp_002689"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGV7851258.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17430..19325)
                     /gene="thiC"
                     /locus_tag="ID725_RS13450"
                     /old_locus_tag="pgaptmp_002690"
     CDS             complement(17430..19325)
                     /gene="thiC"
                     /locus_tag="ID725_RS13450"
                     /old_locus_tag="pgaptmp_002690"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGV7851259.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19406..19506)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19558..20034)
                     /gene="rsd"
                     /locus_tag="ID725_RS13455"
                     /old_locus_tag="pgaptmp_002691"
     CDS             complement(19558..20034)
                     /gene="rsd"
                     /locus_tag="ID725_RS13455"
                     /old_locus_tag="pgaptmp_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGV7851260.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20129..20902
                     /gene="nudC"
                     /locus_tag="ID725_RS13460"
                     /old_locus_tag="pgaptmp_002692"
     CDS             20129..20902
                     /gene="nudC"
                     /locus_tag="ID725_RS13460"
                     /old_locus_tag="pgaptmp_002692"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGV7851261.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20942..22006
                     /gene="hemE"
                     /locus_tag="ID725_RS13465"
                     /old_locus_tag="pgaptmp_002693"
     CDS             20942..22006
                     /gene="hemE"
                     /locus_tag="ID725_RS13465"
                     /old_locus_tag="pgaptmp_002693"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGV7851262.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22016..22687
                     /gene="nfi"
                     /locus_tag="ID725_RS13470"
                     /old_locus_tag="pgaptmp_002694"
     CDS             22016..22687
                     /gene="nfi"
                     /locus_tag="ID725_RS13470"
                     /old_locus_tag="pgaptmp_002694"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGV7851263.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22730..23320
                     /gene="yjaG"
                     /locus_tag="ID725_RS13475"
                     /old_locus_tag="pgaptmp_002695"
     CDS             22730..23320
                     /gene="yjaG"
                     /locus_tag="ID725_RS13475"
                     /old_locus_tag="pgaptmp_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGV7851264.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            23507..23779
                     /gene="hupA"
                     /locus_tag="ID725_RS13480"
                     /old_locus_tag="pgaptmp_002696"
     CDS             23507..23779
                     /gene="hupA"
                     /locus_tag="ID725_RS13480"
                     /old_locus_tag="pgaptmp_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGV7851265.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23792..24487
                     /gene="yjaH"
                     /locus_tag="ID725_RS13485"
                     /old_locus_tag="pgaptmp_002697"
     CDS             23792..24487
                     /gene="yjaH"
                     /locus_tag="ID725_RS13485"
                     /old_locus_tag="pgaptmp_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGV7851266.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHFLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24489..24914)
                     /gene="zraP"
                     /locus_tag="ID725_RS13490"
                     /old_locus_tag="pgaptmp_002698"
     CDS             complement(24489..24914)
                     /gene="zraP"
                     /locus_tag="ID725_RS13490"
                     /old_locus_tag="pgaptmp_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGV7851267.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25152..26528
                     /gene="zraS"
                     /locus_tag="ID725_RS13495"
                     /old_locus_tag="pgaptmp_002699"
     CDS             25152..26528
                     /gene="zraS"
                     /locus_tag="ID725_RS13495"
                     /old_locus_tag="pgaptmp_002699"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGV7851268.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            26525..27850
                     /gene="zraR"
                     /locus_tag="ID725_RS13500"
                     /old_locus_tag="pgaptmp_002700"
     CDS             26525..27850
                     /gene="zraR"
                     /locus_tag="ID725_RS13500"
                     /old_locus_tag="pgaptmp_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGV7851269.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27847..29136)
                     /gene="purD"
                     /locus_tag="ID725_RS13505"
                     /old_locus_tag="pgaptmp_002701"
     CDS             complement(27847..29136)
                     /gene="purD"
                     /locus_tag="ID725_RS13505"
                     /old_locus_tag="pgaptmp_002701"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGV7851270.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29148..30737)
                     /gene="purH"
                     /locus_tag="ID725_RS13510"
                     /old_locus_tag="pgaptmp_002702"
     CDS             complement(29148..30737)
                     /gene="purH"
                     /locus_tag="ID725_RS13510"
                     /old_locus_tag="pgaptmp_002702"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGV7851271.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7311 a         8055 c         8523 g         7238 t
ORIGIN      
        1 ccgatcgaag acgtattctc catctccggt cgtggtaccg ttgttaccgg tcgtgtagaa
       61 cgcggtatca tcaaagttgg tgaagaagtt gaaatcgttg gtatcaaaga gactcagaag
      121 tctacctgta ctggcgttga aatgttccgc aaactgctgg acgaaggccg tgctggtgag
      181 aacgtaggtg ttctgctgcg tggtatcaaa cgtgaagaaa tcgaacgtgg tcaggtactg
      241 gctaagccgg gcaccatcaa gccgcacacc aagttcgaat ctgaagtgta cattctgtcc
      301 aaagatgaag gcggccgtca tactccgttc ttcaaaggct accgtccgca gttctacttc
      361 cgtactactg acgtgactgg taccatcgaa ctgccggaag gcgtagagat ggtaatgccg
      421 ggcgacaaca tcaaaatggt tgttaccctg atccacccga tcgcgatgga cgacggtctg
      481 cgtttcgcaa tccgtgaagg cggccgtacc gttggcgcgg gcgttgtagc aaaagttctg
      541 agctaattgc cgataacatt tgacgcaatg cgcactaaaa gggcatcatt tgatgccctt
      601 tttgcacgct ttcgtaccag aacctggctc atcagtgatt ttctttgtca taatcattgc
      661 tgagacaggc tctgttgagg gcgtataatc cgaaaagcta atacgcgttt cgatttggtt
      721 tgcctcgcga tcgcggggtg aaaatgtttg tagaaaactt ctgacaggtt ggtttatgag
      781 tgcgaatacc gaagctcaag gaagcgggcg cggcctggaa gcgatgaagt gggtcgttgt
      841 ggtggcattg ctcttggtgg cgattgtcgg caactatctt tatcgcgaca ttatgctgcc
      901 gctgcgtgcg ctggccgtag taattctgat tgctgcagcg ggtggtgtcg cgctgttaac
      961 gacaaaaggt aaagctaccg ttgcttttgc ccgtgaagcg cgtaccgaag tccgtaaggt
     1021 catttggccg actcgccagg aaacattgca caccacgctg attgtggctg cggttaccgc
     1081 agtaatgtca ctgatcctgt ggggactgga tggtattctg gttcgcctgg tatcctttat
     1141 cactggcctg aggttctgag atgtctgaag ctcctaaaaa gcgctggtac gtcgttcagg
     1201 cgttttccgg ttttgaaggc cgcgtagcaa cgtcgctgcg tgagcatatc aaattacaca
     1261 acatggaaga tttgtttggt gaagtcatgg taccaaccga agaagtggtt gaaatccgtg
     1321 gcggtcagcg tcgcaaaagc gaacgtaaat tcttccctgg ctacgtcctc gttcagatgg
     1381 tgatgaacga cgcgagctgg cacctggtgc gcagcgtacc gcgtgtgatg ggcttcatcg
     1441 gcggtacttc cgatcgtcct gcgccaatca gcgataaaga agtcgatgcg attatgaacc
     1501 gcctgcagca ggttggtgat aagccgcgtc cgaaaacgct gtttgaaccg ggtgaaatgg
     1561 tccgtgttaa tgatggtccg ttcgctgact tcaacggtgt tgttgaagaa gtggattacg
     1621 agaaatctcg tctgaaagtg tctgtttcta tcttcggtcg tgcgaccccg gtagagctgg
     1681 acttcagcca ggttgaaaaa gcctaaccca gcgatcaaaa aagcggcgat ttaatcgttg
     1741 cacaaggcgc gagattggaa tacaatttcg cgccttttgt ttttatgggt cttgcccgta
     1801 aaacgatttt ttatatcacg gggagcctct aagaggcgtt attacccaac ttgaggaatt
     1861 tataatggct aagaaagtac aagcctatgt caagctgcag gttgcagctg gtatggctaa
     1921 cccgagtccg ccagtaggtc cggctctggg tcagcagggc gtaaacatca tggaattctg
     1981 caaagcgttc aacgcaaaaa ctgattccat cgaaaaaggt ctgccgattc cggtagtaat
     2041 caccgtttac gctgaccgtt ctttcacttt cgttaccaag actccgccgg cagcagttct
     2101 gctgaaaaaa gcggctggta tcaagtctgg ttccggtaag ccgaacaaag acaaagtggg
     2161 taaaatttcc cgcgctcagc tgcaggaaat cgcgcagacc aaagctgccg acatgactgg
     2221 tgccgacatt gaagcgatga ctcgctccat cgaaggtact gcacgttcca tgggcctggt
     2281 agtggaggac taagaaatgg ctaaactgac caagcgcatg cgtgttatcc gcgagaaagt
     2341 tgatgcaacc aaacagtacg acatcaacga agctatcgct ctgctgaaag agctggcgac
     2401 tgctaaattc gtagaaagcg tggacgtagc tgttaacctc ggcatcgacg ctcgtaaatc
     2461 tgaccagaac gtacgtggtg caactgtact gccgcacggt actggccggt ccgttcgcgt
     2521 agccgtattt acccagggtg caaacgctga agctgctaaa gctgcaggtg ctgaactggt
     2581 aggtatggaa gatctggctg accagatcaa gaaaggcgaa atgaactttg acgttgttat
     2641 tgcttctccg gatgcaatgc gcgttgttgg ccagctgggc caggttctgg gtccgcgcgg
     2701 cctgatgcca aacccgaaag tgggtactgt aacgccgaac gttgctgaag cggttaaaaa
     2761 cgctaaagct ggccaggttc gttaccgtaa cgacaaaaac ggcatcatcc acaccaccat
     2821 cggtaaagtg gactttgacg ctgacaaact gaaagaaaac ctggaagctc tgctggttgc
     2881 gctgaaaaaa gcaaaaccga ctcaggcgaa aggcgtgtac atcaagaaag ttagcatctc
     2941 caccaccatg ggtgcaggtg ttgcagttga ccaggctggc ctgagcgctt ctgtaaacta
     3001 atgcctttac gtgggcggtg attttgtcta caatcttacc cccacgtatg ctaacgaaag
     3061 ttagcagcta aaaagatttg ttcgttggag cctggcctat ccaggcctcc gtcgaagacc
     3121 gcaggagttt cgcaagaaac ttaatcccct gcgtagacgg tgacagaacg ctaagattat
     3181 tcttttatat tctggcttgt ttctgctcac cgtaattaag acgctctctc cgtttggagg
     3241 agtgaagtga gttccagaga ttttctctgg caaacatcca ggagcaaagc taatggcttt
     3301 aaatcttcaa gacaaacaag cgattgttgc tgaagtcagc gaagtagcca aaggcgcgct
     3361 gtctgcagta gttgcggatt cccgtggcgt aactgtagat aaaatgactg aactgcgtaa
     3421 agcaggtcgc gaagctggcg tatacatgcg tgttgttcgt aacaccctgc tgcgccgtgc
     3481 tgttgaaggt actccgttcg agtgcctgaa agacgcgttt gttggtccga ccctgattgc
     3541 atactctatg gaacacccgg gcgctgctgc tcgtctgttc aaagagttcg cgaaagcgaa
     3601 tgcaaaattt gaggtcaaag ccgctgcctt tgaaggtgag ctgatcccgg cgtctcagat
     3661 cgaccgcctg gcaactctgc cgacctacga agaagcaatt gcacgcctga tggcaaccat
     3721 gaaagaagct tcggctggca aactggttcg tactctggct gctgtacgcg atgcgaaaga
     3781 agctgcttaa tcgcagttat ctttttaacg cattcgctta cgtataaact tattctgata
     3841 ttcaggaaca atttaaatgt ctatcactaa agatcaaatc attgaagcag ttgcagctat
     3901 gtctgtaatg gacgttgtag aactgatctc tgcaatggaa gaaaaattcg gtgtttccgc
     3961 tgctgctgct gtagctgtag ctgctggccc ggttgaagct gctgaagaaa aaactgaatt
     4021 cgacgtaatt ctgaaagctg ctggcgctaa caaagttgct gttatcaaag cagtacgtgg
     4081 cgcaactggc ctgggtctga aagaagctaa agacctggta gaatctgcac cggctgctct
     4141 gaaagaaggc gtgagcaaag acgacgcaga agcactgaaa aaagctctgg aagaagctgg
     4201 cgctgaagtt gaagttaaat aagccaaccc ttccggttgc agcctgagaa atcaggctga
     4261 tggctggtga ctttttagtc accagccttt ttgcgctgta aggcaccagt agcgtttcac
     4321 actgtttgac tactgctgtg cctttcaatg cttgtttcta tcgacgactt aatatactgc
     4381 gacaggacgt ccgttctgtg taaatcgcaa tgaaatggtt taagcgtgat agcaacaggc
     4441 attgcggaaa gtgttccatt ttccggtcaa caaaatagtg ttgcacaaac tgtccgctca
     4501 atggacagat gggtcgactt gtcagcgagc tgaggaaccc tatggtttac tcctataccg
     4561 agaaaaaacg tattcgtaag gattttggta aacgtccaca agttctggat gtaccttatc
     4621 tcctttctat ccagcttgac tcgtttcaga aatttatcga gcaagatcct gaagggcagt
     4681 atggtctgga agctgctttc cgttccgtat tcccgattca gagctacagc ggtaattccg
     4741 agctgcaata cgtcagctac cgccttggcg aaccggtgtt tgacgtccag gaatgtcaaa
     4801 tccgtggcgt gacctattcc gcaccgctgc gcgttaaact gcgtctggtg atctatgagc
     4861 gcgaagcgcc ggaaggcacc gtaaaagaca ttaaagaaca agaagtctac atgggcgaaa
     4921 ttccgctcat gacagacaac ggtacctttg ttatcaacgg tactgagcgt gttatcgttt
     4981 cccagctgca ccgtagtccg ggcgtcttct ttgactccga caaaggtaaa acccactctt
     5041 cgggtaaagt gctgtataac gcgcgcatca tcccttaccg tggttcctgg ctggacttcg
     5101 aattcgatcc gaaggacaac ctgttcgtac gtatcgaccg tcgccgtaaa ctgcctgcga
     5161 ccatcattct gcgtgccctg aactacacca cagagcagat cctcgacctg ttctttgaaa
     5221 aagttatctt tgaaatccgt gataacaagc tgcagatgga actggtgccg gaacgcctgc
     5281 gtggtgaaac cgcatccttt gacatcgaag ctaacggtaa agtgtacgta gaaaaaggcc
     5341 gccgtatcac tgcgcgccac attcgccagc tggaaaaaga cgacgtcaaa ctgatcgaag
     5401 tcccggttga gtacatcgca ggtaaagtgg ttgctaaaga ctatattgat gagtctaccg
     5461 gcgagctgat ctgcgcagcg aacatggagc tgagcctgga tctgctggct aagctgagcc
     5521 agtctggtca caagcgtatc gaaacgctgt tcaccaatga tctggatcac ggcccgtata
     5581 tctctgaaac cttacgtgtc gacccaacta acgaccgtct gagcgcactg gtagaaatct
     5641 accgcatgat gcgccctggc gagccgccga ctcgtgaagc agcggaaagc ctgttcgaga
     5701 acctgttctt ctccgaagac cgttatgacc tgtctgcggt tggtcgtatg aagttcaacc
     5761 gttctctgct gcgcgaagaa atcgaaggtt ctggtatcct gagcaaagac gacatcattg
     5821 atgttatgaa aaagctcatc gatatccgta acggtaaagg cgaagtcgat gatatcgacc
     5881 acctcggcaa ccgtcgtatc cgttccgttg gcgaaatggc ggaaaaccag ttccgcgttg
     5941 gcctggtacg tgtagagcgt gcggtgaaag agcgtctgtc tctgggcgat ctggataccc
     6001 tgatgcctca ggatatgatc aacgccaagc cgatttccgc agcagtgaaa gagttcttcg
     6061 gttccagcca gctgtctcag tttatggacc agaacaaccc gctgtctgag attacgcaca
     6121 aacgtcgtat ctccgcactc ggcccaggcg gtctgacccg tgaacgtgca ggcttcgaag
     6181 ttcgagacgt acacccgact cactacggtc gcgtatgtcc aatcgaaacc cctgaaggtc
     6241 cgaacatcgg tctgatcaac tctctgtccg tgtacgcaca gactaacgaa tacggcttcc
     6301 ttgagactcc gtatcgtaaa gtgactgacg gtgttgtaac tgacgaaatt cactacctgt
     6361 ctgctatcga agaaggcaac tacgttatcg cccaggcgaa ctccaacctg gatgaagaag
     6421 gccacttcgt agaagacctg gtaacctgcc gtagcaaagg cgaatccagc ttgttcagcc
     6481 gtgaccaggt tgactacatg gacgtatcca cccagcaggt ggtatccgtc ggtgcgtccc
     6541 tgatcccgtt cctggaacac gatgacgcca accgtgcatt gatgggtgcg aacatgcaac
     6601 gtcaggccgt tccgactctg cgtgctgata agccgctggt tggtactggt atggaacgtg
     6661 ctgttgccgt tgactccggt gtaactgcgg ttgctaaacg tggtggtgtc gttcagtacg
     6721 tggatgcttc ccgtatcgtt atcaaagtta acgaagacga gatgtatccg ggtgaagcag
     6781 gtatcgacat ctacaacctg accaaataca cccgttctaa ccagaacacc tgtattaacc
     6841 agatgccgtg tgtgtctctg ggtgaaccgg ttgaacgtgg cgacgtgctg gcagacggtc
     6901 cgtccaccga ccttggtgaa ctggcgcttg gtcagaacat gcgcgtagcg ttcatgccgt
     6961 ggaatggtta caacttcgaa gactccatcc tcgtatccga gcgtgttgtt caggaagacc
     7021 gtttcaccac catccacatt caggaactgg cgtgtgtgtc ccgtgacacc aagctggggc
     7081 cagaagagat caccgctgac atcccgaacg tgggtgaagc tgcgctctcc aaactggatg
     7141 aatccggtat cgtttatatt ggtgcggaag tgaccggtgg cgacattctg gttggtaagg
     7201 ttacgccgaa aggtgaaact cagctgaccc cagaagaaaa actgctgcgt gcgatcttcg
     7261 gtgagaaagc gtctgacgtt aaagactctt ctctgcgcgt accaaacggt gtatccggta
     7321 cggttatcga cgttcaggtc tttactcgcg atggcgtaga aaaagacaaa cgtgcgctgg
     7381 aaatcgaaga aatgcagctc aaacaggcga agaaagacct gtctgaagaa ctgcagatcc
     7441 tcgaagcggg tctgttcagc cgtatccgtg ctgtgctggt agccggtggc gttgaagctg
     7501 agaagctcga caaattgccg cgcgatcgct ggctggagct gggcctgacc gacgaagaga
     7561 aacaaaatca gctggaacag ctggctgagc agtatgacga actgaaacac gagttcgaga
     7621 agaaactcga agcgaaacgc cgcaaaatca cccagggcga cgatctggca ccgggcgtgc
     7681 tgaagattgt taaggtatat ctggcggtta aacgccgtat ccagcctggt gacaagatgg
     7741 caggtcgtca cggtaacaag ggtgtaattt ctaagatcaa cccgatcgaa gatatgcctt
     7801 acgatgaaaa cggtacgccg gtagacatcg tactgaaccc gctgggcgta ccgtctcgta
     7861 tgaacatcgg tcagatcctc gaaacccact tgggtatggc tgcgaaaggt atcggcgaca
     7921 agatcaacgc catgctgaaa cagcagcagg aagtcgcgaa actgcgtgaa ttcatccagc
     7981 gtgcgtacga tctgggcgct gacgttcgtc agaaagttga cctgagtacc ttcagcgatg
     8041 aagaagttat gcgtctggct gaaaacctgc gcaaaggtat gccaatcgca acgccggtgt
     8101 tcgacggtgc gaaagaagca gaaattaaag agctgctgaa acttggcgac ctgccgactt
     8161 ctggtcagat ccgcctgtac gacggccgca ctggtgaaca gttcgaacgt ccggtaaccg
     8221 ttggttacat gtacatgctg aaactgaacc acctggtcga cgacaagatg cacgcgcgtt
     8281 ccaccggttc ttacagcctg gttactcagc agccgctggg tggtaaggca cagttcggtg
     8341 gtcagcgttt cggggagatg gaagtgtggg cgctggaagc atacggcgca gcatacaccc
     8401 tgcaggaaat gctcaccgtt aagtctgatg acgtgaacgg tcgtactaag atgtataaaa
     8461 acatcgtgga cggcaaccat cagatggagc cgggcatgcc agaatccttc aacgtattgt
     8521 tgaaagagat tcgttcgctg ggtatcaaca tcgaactgga agacgagtaa ttctcgctca
     8581 aacaggtcac tgctgtcggg gtaaaacccg gcagcggatt gtgctaactc cgacgggagc
     8641 aaatccgtga aagatttatt aaagtttctg aaagcgcaga ctaaaaccga agagtttgat
     8701 gcgatcaaaa ttgctctggc ttcgccagac atgatccgtt catggtcttt cggtgaagtt
     8761 aaaaagccgg aaaccatcaa ctaccgtacg ttcaaaccag aacgtgacgg ccttttctgc
     8821 gcccgtatct ttgggccggt aaaagattac gagtgcctgt gcggtaagta caagcgcctg
     8881 aaacaccgtg gcgtcatctg tgagaagtgt ggcgttgaag tgacccagac taaagtacgc
     8941 cgtgagcgta tgggccacat cgaactggct tccccgactg cgcacatctg gttcctgaaa
     9001 tcgctgccgt cccgtatcgg cctgctgctc gatatgccgc tgcgcgatat cgaacgcgta
     9061 ctgtactttg aatcctatgt ggttatcgaa ggcggtatga ccaacctgga gcgtcagcag
     9121 atcctgactg aagagcagta tctggacgcg ctggaagagt tcggtgacga attcgacgcg
     9181 aaaatgggtg cggaagcaat ccaggccctg ctgaagagca tggatctgga gcaagagtgc
     9241 gaacagctgc gtgaagagct gaacgaaacc aactccgaaa ccaagcgtaa aaagctgacc
     9301 aagcgtatca aactgctgga agcgttcgtt cagtctggta acaaaccaga gtggatgatc
     9361 ctgaccgttc tgccggtact gccgccagat ctgcgtccgc tggttccgct ggatggtggt
     9421 cgtttcgcga cttctgacct gaacgatctg tatcgtcgcg tcattaaccg taacaaccgt
     9481 ctgaaacgtc tgctggatct ggctgcgccg gacatcatcg tacgtaacga aaaacgtatg
     9541 ctgcaggaag cggtagacgc cctgctggat aacggtcgtc gcggtcgtgc gatcaccggt
     9601 tctaacaagc gtcctctgaa atctttggcc gacatgatca aaggtaaaca gggtcgtttc
     9661 cgtcagaacc tgctcggtaa gcgtgttgac tactccggtc gttctgtaat caccgtgggt
     9721 ccatacctgc gtctgcatca gtgcggtctg ccgaagaaaa tggcgctgga gctgttcaaa
     9781 ccgttcatct acggcaagct ggaactgcgt ggtcttgcta ccaccattaa agctgcgaag
     9841 aaaatggttg agcgcgaaga agctgtcgtt tgggatatcc tggacgaagt tatccgcgaa
     9901 cacccggtac tgctgaaccg tgcaccgact ctgcaccgtc tgggtatcca ggcatttgaa
     9961 ccggtactga tcgaaggtaa agctatccag ctgcacccgc tggtttgtgc ggcatataac
    10021 gccgacttcg atggtgacca gatggctgtt cacgtaccgc tgacgctgga agcccagctg
    10081 gaagcgcgtg cgctgatgat gtctaccaac aacatcctgt ccccggcgaa cggcgaacca
    10141 atcatcgttc cgtctcagga cgttgtactg ggtctgtact acatgacccg tgactgtgtt
    10201 aacgccaaag gcgaaggcat ggtgctgact ggcccgaaag aagcagaacg tctgtatcgc
    10261 tctggtctgg cttctctgca tgcgcgcgtt aaagtgcgta tcaccgagta tgaaaaagat
    10321 gctaacggtg aattagtagc gaaaaccagc ctgaaagaca cgactgttgg ccgtgccatt
    10381 ctgtggatga ttgtaccgaa aggtctgcct tactccatcg tcaaccaggc gctgggtaaa
    10441 aaagcaatct ccaaaatgct gaacacctgc taccgcattc tcggtctgaa accgaccgtt
    10501 atttttgcgg accagatcat gtacaccggc tttgcatatg cagcgcgttc tggtgcatct
    10561 gttggtatcg atgacatggt catcccggag aagaaacacg aaatcatctc cgaggcagaa
    10621 gcagaagttg ctgaaattca ggagcagttc cagtctggtc tggtaactgc gggcgaacgc
    10681 tacaacaaag ttatcgatat ctgggctgcg gcgaacgatc gtgtatccaa agcgatgatg
    10741 gataacctgc aaactgaaac cgttattaac cgtgacggtc aggaagagaa gcaggtttcc
    10801 ttcaacagca tctacatgat ggccgactcc ggtgcgcgtg gttctgcggc acagattcgt
    10861 cagcttgctg gtatgcgtgg tctgatggcg aagccggatg gctccatcat cgaaacgcca
    10921 atcaccgcga acttccgtga aggtctgaac gtactccagt acttcatctc cacccacggt
    10981 gctcgtaaag gtctggcgga taccgcactg aaaactgcga actccggtta cctgactcgt
    11041 cgtctggttg acgtggcgca ggacctggtg gttaccgaag acgattgtgg tacccatgaa
    11101 ggtatcatga tgactccggt tatcgagggt ggtgacgtta aagagccgct gcgcgatcgc
    11161 gtattgggtc gtgtaactgc tgaagacgtt ctgaagccgg gtactgctga tatcctcgtt
    11221 ccgcgcaaca cgctgctgca cgaacagtgg tgtgacctgc tggaagagaa ctctgtcgac
    11281 gcggttaaag tacgttctgt tgtatcttgt gacaccgact ttggtgtatg tgcgcactgc
    11341 tacggtcgtg acctggcgcg tggccacatc atcaacaagg gtgaagcaat cggtgttatc
    11401 gcggcacagt ccatcggtga accgggtaca cagctgacca tgcgtacgtt ccacatcggt
    11461 ggtgcggcat ctcgtgcggc tgctgaatcc agcatccagg tgaaaaacaa aggtagcatc
    11521 aagctcagca acgtgaagtc ggttgtgaac tccagcggta aactggttat cacttcccgt
    11581 aacaccgaac tgaaactgat cgacgaattc ggtcgtacca aagaaagcta caaagtacct
    11641 tacggtgcgg tactggcaaa aggcgatggc gaacaggttg ctggcggcga aaccgttgca
    11701 aactgggacc cgcacaccat gccggttatc accgaagtaa gcggttttgt acgctttact
    11761 gacatgatcg acggccagac cattactcgt cagaccgacg aactgaccgg tctgtcttcg
    11821 ctggtggttc tggattccgc agaacgtacc gcaggtggta aagatctgcg tccggcactg
    11881 aaaatcgttg atgctcaggg taacgacgtt ctgatcccag gtaccgatat gcctgcgcag
    11941 tacttcctgc cgggtaaagc gattgttcag ctggaagatg gcgtacagat cagctctggt
    12001 gacaccctgg cgcgtattcc gcaggaatcc ggcggtacca aggacatcac cggtggtctg
    12061 ccacgcgttg cggacctgtt cgaagcacgt cgtccgaaag agccggcaat cctggctgaa
    12121 atcagcggta tcgtttcctt cggtaaagaa accaaaggta aacgtcgtct ggttatcacc
    12181 ccggtagacg gtagcgatcc gtacgaagag atgattccga aatggcgtca gctcaacgtg
    12241 ttcgaaggtg aacgtgtaga acgtggtgac gtaatttccg acggtccgga agcgccgcac
    12301 gacattctgc gtctgcgtgg tgttcatgct gtgactcgtt acatcgttaa cgaagtacag
    12361 gacgtatacc gtctgcaggg cgttaagatt aacgataaac acatcgaagt tatcgttcgt
    12421 cagatgctgc gtaaagctac catcgttaac gcgggcagct ccgacttcct ggaaggcgaa
    12481 caggttgaat actctcgcgt caagatcgca aaccgcgaac tggaagcgaa cggcaaagtg
    12541 ggtgcaactt actcccgcga tctgctgggt atcaccaaag cgtctctggc aaccgagtcc
    12601 ttcatctccg cggcatcgtt ccaggagacc actcgcgtgc tgaccgaagc agccgttgcg
    12661 ggcaaacgcg acgaactgcg cggcctgaaa gagaacgtta tcgtgggtcg tctgatcccg
    12721 gcaggtaccg gttacgcgta ccaccaggat cgtatgcgtc gccgtgctgc gggtgaagct
    12781 ccggctgcac cgcaggtgac tgcagaagac gcatctgcca gcctggcaga actgctgaac
    12841 gcaggtctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg
    12901 cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag
    12961 gaatttctga atactcataa tcaatgtaga gatgactaat atcctgaaac tgactgaact
    13021 aattgagtca aactcggcaa ggattcgata ctattcctgt gtagctttct taaggaacga
    13081 gaatgaaaca ggaagtggag aagtggcgac cttttaaaca tccggatggc gacgttcgtg
    13141 atttatcgtt tcttgatgct catcaggtta tctacgttca gcatcatgag gacaaggagc
    13201 ctttagagta tcgcttttgg gttacctact ctcttcactg cttcacaaaa gattatgaac
    13261 atcagacgaa cgaagaaaaa ctatcgttaa tgtaccacgc gcctaaagaa tctcgtccct
    13321 tctgccagca ccgttataac ttagcgcgca cacacttaaa aagaactatt ttggcgctgc
    13381 cagaaagcaa cgttattcat gccgggtatg gtagctatgc cgtgattgag gtggacttag
    13441 acggaggaga taaggcattt tactttgttg tgttcagggc tttcagtgaa atttttcacc
    13501 attgcctaaa acttattgag aaataagcag cttcctcagc cctcaaaata acaaaaccca
    13561 ccttaaggtg ggtttcgcca gagaattatc tctggtattc agaacgccat taccggactt
    13621 tgccttgacc ttgcgataat cgcaggttgc gggatgtctg aatttcttca gtctgctgca
    13681 tcctggaaga tgagaacatg tgttcttatt ttcgtctcta tcatagttga gtatttactc
    13741 tcttacaatc agatctcttt cattgctcaa caggcgatgg cttcagactt tgcattacgg
    13801 aatttttaag aaaggcaggg cgaaacgagg aagaagcttt tcgccctgga caacattcct
    13861 gctgacggca ctaccataaa aaaacacaac gctttcaaca accttgcgtt tttcatcttc
    13921 cgtagcggct cggaataaat tttcgttctt acattccgtc tcatagtctt tgcgaggcgc
    13981 gtcccagata gctgtcccag tctttccata ccggctgcaa accctgagcg gttaacgcgg
    14041 cagcaaccgc ttccggtctg cgctcgtcgt gcggtgagaa ctgttccagc tcggggtgat
    14101 tgtcggcata gccacctggc tgcgttttcg aaaaagcgct gacgttatta attgccagcg
    14161 gaataacgcg atcgcgaaac cacggtgatt cccgcgtgga gagtgacagt tcaatctccg
    14221 gtgcaagcag ccggaaggcg cagatggttt gcactaactg gcgttcatcc ataatcgacg
    14281 caggctcaat gccgccagta catggacgca ggcgtgggaa ggagacagag tagcggcttt
    14341 gccagtaatg ctgttgcagc catagcaaat gttctgcaac catatagcag tcaacgcgcc
    14401 agctgtcgga aaggccaatt agcgcgccga ggcctatctt atcaatcccc gcacggccca
    14461 gccgatccgg cgtttccagc cgccagaaga agtcctgttt tttgcctttc agatgatggc
    14521 gggcataagt cgcctcgtga tatgtctcct gataaaccat cacgccatcc agaccaagtt
    14581 gctttaactc ggcgtattcc gtctccgcca gcggctgtac ttccatctgt agtgaagaga
    14641 actgttcacg cagggcaggg agatgacgac gaaagtaatc catccccact ttcgcctgat
    14701 gttcaccggt gactaaaagc agatgttcaa agcccatctc ccgtatagcg gcgctttccc
    14761 tggcaatatc cgcttcatcc agcgttttgc gcttgatgcg gttgctcata gagaatccgc
    14821 agtacgtgca gtcgttagcg caaagattgg aaagataaag cgggacgtag aaactaactg
    14881 tgttgccaaa tcgctgacgg gtcagacgct gcgcccgttg ggccagttgt tccagatagc
    14941 cactggcggc tggcgataac agcgccatca tatcgtcgcg ggtgagttgc gaggcattta
    15001 gcgcccgctc tacgtcagca gccgttttgc cgttgatacg caggcggatg tcgtcccagt
    15061 ccagttgtcg ccagcgatcg ctgaaggttt tcataccgat gcctccagaa atccggtcag
    15121 cgggctggtg gcatgagcaa aataactgcg gctgcccggt ccggactgag gtgccagcag
    15181 gcctgcttct actgccagac gaaatgcctt cgccatgttg acgggatcgt ccgcgacggc
    15241 aatcgccgta ttcactaaca ccgcgtcggc ccccatttcc agcgcctgcg cggcatggct
    15301 gggaacgccg atgccagcat cgacaaccac cggaactgtg gcctgctgga taataatctc
    15361 cagcatggcg cgggtttcca gcccctgatt cgagccaatc ggcgcgccga gcggcatcac
    15421 cgctgcacag ccgacttctt ccagacgttt acacaatacc ggatcggccc cgcagtaagg
    15481 cagcacgaca aatccctgtt gtaccagcgt ttcggcggct ttcagggttt cgatgggatc
    15541 gggcaacagc cagcgggcgt cagggtgaat ctccaatttt aaccagtttg tgcctaacgc
    15601 ttcacgagcc agatgggcgg cgaaaatggc ttcttccgct gttttcgccc cggatgtatt
    15661 tggcagcagg gtcacacccg ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg
    15721 caagtcgaca cgtttcatcg ccagcgtcac cagctggctg ccggaagcgc ggattgcctc
    15781 taccatcagt tgtgaagagg cgaatttccc ggtgccggta aacagatgtg aatcaaacgt
    15841 tttgtccgca atacgtaaca tctcaacccc ctgcaataac ctgaaaaagt aggatctggt
    15901 cgccatcctg cacgatatgt tgcgtccact gctcacgcgg gacgatttgc tgattaatcg
    15961 ccagagccgc gcccgcttgt cgttgaacga gttgctccag tagttcgtga acagtttgcc
    16021 cggcggcgca ctgcatcggt tgatcgttaa acaggatctg cattgcttcc tccgcatacc
    16081 gggcaaccac tggcgcggcg caacgccagg ctgcgccact ggctcgactt accgtcgaac
    16141 agtcggagtt ctcccgcagg cgtctctata ccgcttaata acttaatggc ttccagtgcc
    16201 tgcaaagtgc ccataacccc gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt
    16261 tctggctcct ggttttctgg ccacaggcag cggtaacaac cctgccccca gggcggcgtc
    16321 agtaccatca actgaccgcc aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt
    16381 gccacgcagg cggcattaat ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc
    16441 acatcggctt gtgcaaccgc atcttttaac gcctcacccg ttaaccgttg ttgtaatgct
    16501 gtcagttgaa tatcgggatt caactgtgtc agtcgctgat ggctgacctg cgatttcggg
    16561 cgatcgatat cttcagtggt aaagaggatt tgtcgttgca gattgcttaa atgcacatcg
    16621 tcgtcatctg ccagtaccag cgtcccgacg ccagcgcccg ccaggtatag cgcagcaggt
    16681 gtacccagcc cgccaagacc gataatcagc acctggctgt cgagcagttt ttgctgcccg
    16741 tcaagagcga tatcgtcgag caggatttgg cggctataac gcataaaatc acggtcattc
    16801 atcgccaact cctgcaattt ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg
    16861 agtaatggcg ctgacgacgg cgatactgcc gacacccgtt gctatcaccg caggcgcgcg
    16921 tgccagactg ataccgccaa tcgccacggt gggataatcc gacagtcgct caacatgccg
    16981 tgccagctgt tccagcccct gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac
    17041 atgtcccagc gcgatataag aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc
    17101 atgggtcgaa acgcccagcc gcaggcctgc cgcgcggatg gcattgagat cggtggcttg
    17161 caaatcttcc tgccccaaat ggacgccata cgcctgatgc ttgatcgcga gccgccagta
    17221 atcgttgata aacaatcgcg cgttatagcg gcggcccagc gcaattgccg ccacgacatc
    17281 ggcttccacc tcttcatcgc gccgatcttt gatgcgtagc tggagagtac gtacgcctgc
    17341 atccaacaga cgttcgatcc actgtacgct gtccaccacc gggtacagtc ctaaacgaaa
    17401 aggtacagga ggaaaatcag gctgatacat cacgcttcct ccttacgcag gtagatttct
    17461 ccgcctctgg cacggaagtt ctccgacata tccgctattc ccatttcaat agtttgcgtg
    17521 gcggcgtaat cacgcacttc ctggctgatt ttcatcgagc agaatttcgg cccacacatg
    17581 gagcaaaaat gggcgacttt acctgactct tgcggcaggg tttcatcgtg ataagcgcgg
    17641 gcggtaaacg ggtcgagggc cagattaaac tggtcttccc agcgaaattc gaagcgggct
    17701 ttcgacatgg cgttatcgcg aatttgtgcg cccggatgcc ctttcgccag gtcagcggcg
    17761 tgggcggcaa tcttataggt gataagcccc tgcttaacat cttctttatt aggcagaccc
    17821 agatgctctt ttggcgttac gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc
    17881 gcaccaatcc ccgacgtgaa gtggtcatag cccggcgcaa tatcggtagt tagcggcccc
    17941 agagtgtaaa acggcgcttc gtggcagtgc tctaactcct cggtcatatt gcggcggatc
    18001 atctgcatcg gcacgtggcc tgggccttca atcatcacct gcacatcata ttcccaggcg
    18061 atttttgtca gttcgcccag cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc
    18121 tgaatagaac cggggcgcag accgtcgccc agcgacagcg aaacgtcata agcggcacag
    18181 atttcacaaa tttcgcggaa gtgttgatag aggaaatttt cctgatgatg ggagaggcac
    18241 catttcgcca taatcgaacc gccgcgcgag acgataccgg tcaggcgttt cgcggtcatc
    18301 ggcacatagc gcagcagcac ccccgcatgg atagtgaagt aatccacacc ttgctcggcc
    18361 tgttccagca gcgtgtcgcg gaacgcttcc caggtaagat cttcggcgat cccattaacc
    18421 ttctccagcg cctggtagat cggcactgta ccaatcggca ccgggctgtt acgcaaaatc
    18481 cactcgcggg tttcgtgaat atagcgaccg gtggagagat ccatcaccgt atccgctccc
    18541 cagcgcgtgg accataccag cttttccact tcttcttcga tggaagaggt gaccgccgag
    18601 ttgccgatat tggcgttaac ttttaccagg aaattgcgac caataatcat cggctccgat
    18661 tccggatgat taatgttggc cgggataatc gcacgtccgg cagcaacttc atcacggaca
    18721 aattccgcag tgatattttc cggcagacgt gcgccaaagc tcattcccgg atgctggtgg
    18781 cgtaaaactt cgctacggat gcgctcgcgg cccatattct cgcggatggc gatgaattcc
    18841 atttccggtg tgatgatgcc ctggcgggcg tagtgcagtt gtgtgacgcg gcgtcctgct
    18901 ttggcgcgtt ttggcgttaa tacgccgcta aaacgcagct cgtcgaggcc atcatctgcc
    18961 agccgcgctt tagtgtaatc ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca
    19021 tcgatccacg gctggcgtag ttttgccagc ccttgctgca cgttaatggc gatctgcgga
    19081 tcgccatacg ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc
    19141 tgttctttgc taccgccaat tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc
    19201 acgccgggtt gtgtgccagt gatataaatg cgttttgagt tgggaaaggc ggtgccttcc
    19261 agggtgtcga taaaatgttg ggcctgggcg cgttgttcgc gacgggtcag ttttgttgca
    19321 gacatagctc attccaaaaa gttaaggacg tggcttgtca gacgacggat gaagcaagag
    19381 acgatcgccg caaaggcgat gcgatagcag attaactctt gttcccttcg caggtattag
    19441 cctgatcagg ttccgcggat cccgaataaa cggtctcagc cagttaaggc actccgacaa
    19501 gaaattagcc ccacaaacgg ggcgttgaat gtaaattacg cgttaacagc gcagaactca
    19561 agcaggatgt ttgacgcggg cggcgtcaag caccagcaga atcagcttat cttccagcac
    19621 aaagcgcgct tccagcgcct cgccgatgtc agataaaacc tgttgaaact caaggtaatt
    19681 atcatgatcg atagcggttt ccaggctgga atcgtagtaa tccataatct gttgggtatt
    19741 ggcttcgagt tgcggccaaa tcttcgcggc gcgcgcgagt tgcccgttgc cttccagctt
    19801 atgaagaatg cgctcataaa tactgaaatg tccggcagac aagtaatcga ccaggctctg
    19861 acaaaaatca tcaagggctt tttcgtttag cctcatgtac gattctttgc caggcttaat
    19921 gccaaccaga ttgtagtaag ccacgagcag atgcttacgt acatgtagcc agcgatcaac
    19981 cagtttgtta cttcctctga cgcgctccgt caggttatcg agctggttaa gcatgattga
    20041 ctccgcaagt ttgtattcaa aaactgctca gtgagaaatg taaaaaccat gttaaacatg
    20101 ccagtgatgc aaaggtagtg caagagctat ggatcgtata attgaaaaat tagatcacgg
    20161 ctggtgggtc gtcagccatg aacaaaaatt atggttgccg aagggagaat tgccatatgg
    20221 cgaagcggca aatttcgatc ttgtgggtca gcgcgcacta caaatcggcg aatggcaggg
    20281 ggaacctgtt tggttaatac aacagcagcg gcgttacgat atggggtcgg tacgtcaggt
    20341 cattgatctc gatgttgggc tgtttcaact ggccggacgt ggcgtacaac tggcggagtt
    20401 ttaccgatcg cataaatact gtggttactg cgggcatgaa atgtatccaa gcaaaaccga
    20461 atgggcgatg ctgtgcagcc attgccgtga gcgttactac ccgcaaatcg ccccctgcat
    20521 tattgttgcc atccgtcgcg atgattcgat cctcctcgcc cagcataccc gccatcgtaa
    20581 cggtgtccat acggtacttg ccggattcgt cgaagtgggc gaaaccctcg agcaggcggt
    20641 cgcgcgggaa gtgatggaag agagcggaat taaagttaaa cacttgcgtt atgtgacttc
    20701 ccagccgtgg ccgtttcctc agtctttaat gaccgcgttt atggcggaat atgacagcgg
    20761 cgacatcgtg atcgacccga aagaattgct cgaggcgaac tggtatcgct atgacgattt
    20821 gccgttactc ccgccgcccg gcaccgtagc gcgccgtctg atagaagata cggtggcgat
    20881 gtgtcgggca gagtatgagt gatgatacac tgaccgcctg acgcactaag gaacagccaa
    20941 aatgaccgaa cttaaaaacg atcgttatct gcgggcgctg ctgcgccagc ccgttgatgt
    21001 cactcctgta tggatgatgc gccaggcggg tcgctatcta ccggaatata aagccacgcg
    21061 cgcccaggcg ggcgatttta tgtcgctgtg caaaaacgcc gagctggcgt gtgaagtgac
    21121 cttgcaaccg ctgcgtcgct acccgctgga tgcggcgatc ctcttttccg atatcctcac
    21181 cgtcccggac gcgatggggt tagggctcta ttttgaagcc ggagaaggtc cgcgttttac
    21241 ctcgccagtc acctgcaaag ctgacgtcga taaactgcca attccggacc cggaagatga
    21301 actggggtac gtgatgaacg cggtgcgtac cattcgtcgc gaactgaaag gcgaagtgcc
    21361 gctgattggt ttttccggca gcccgtggac gctggcgacc tacatggtgg aaggcggcag
    21421 cagcaaagcc ttcaccgtga tcaaaaaaat gatgtatgcc gatccgcagg cgctgcacgc
    21481 tctactcgat aaactggcga aaagcgtcgc tttgtatctg aatgcgcaga ttaaagccgg
    21541 tgctcaggct gtgatgattt tcgacacctg gggcggcgtg cttaccgggc gcgattatca
    21601 acagttctcg ctttattaca tgcataaaat tgttgatggt ttactgcgtg aaaacgacgg
    21661 tcgccgcgta ccggtcacgc tgtttaccaa aggcggcgga cagtggctgg aagccatggc
    21721 agaaaccggt tgcgatgcgc tgggcctcga ctggacaacg gacatcgccg atgcgcgccg
    21781 ccgtgtgggc aataaagtcg cgttgcaggg taatatggat ccgtcgatgc tgtacgcgcc
    21841 gcctgcccgc attgaagaag aagtagcgac tatacttgca ggtttcggtc acggcgaagg
    21901 tcatgtcttt aaccttggtc acggcattca tcaggatgtg ccgccagaac atgctggcgt
    21961 gttcgtggag gcagtgcatc gactgtctga acagtatcac cgctaaggag cgattatgga
    22021 tctcgcgtca ttacgcgctc aacaaatcga actggcttct tctgtgatcc gcgaggatcg
    22081 actcgataag gatccgccgg atctgatcgc cggagccgat gtcgggtttg agcagggcgg
    22141 agaagtgacg cgagcggcga tggtgctgct gaaatacccc tcgcttgagc tggtggagta
    22201 taaagtggcc cgcatcgcta ccaccatgcc ttacattcca ggttttcttt ccttccgcga
    22261 atatcctgcg ctgctggcag cgtgggagat gctgtcacaa aagccggatt tagtgtttgt
    22321 cgatggtcat gggatctcgc atcctcgccg tcttggcgtc gccagccatt ttggtttgct
    22381 ggtggatgtg ccgaccattg gcgtagcgaa aaaacgacta tgcggtaaat tcgaaccgct
    22441 ctccagcgaa ccgggcgcgc tggccccact gatggataaa ggcgagcagc tggcctgggt
    22501 ctggcgcagc aaagcgcgct gtaacccgct atttatcgct actggtcatc gggtcagcgt
    22561 ggacagcgcg ctggcgtggg tacaacgctg catgaaaggc tatcgtctgc cggagccaac
    22621 gcgctgggcg gacgcggtgg cctcggaacg tccggcgttc gtgcgctata cagcaaatca
    22681 gccctaattc aggtaaactg cggataattt ccgtatttga gaactcaaca tgttacaaaa
    22741 cccaattcat ctgcgtctgg aacgcctgga aagctggcag catgtcactt tcatggcttg
    22801 cttatgcgaa cgcatgtacc ccaattacgc catgttctgc cagcaaaccg gttttggtga
    22861 tgggcaaatt taccgtcgta ttctcgatct catctgggaa acgctgaccg ttaaagatgc
    22921 aaaagtaaat ttcgacagcc aactggagaa atttgaagaa gcgattcctt cagccgacga
    22981 tttcgatctg tacggcgttt atccggcaat cgatgcctgc gtggcgttaa gtgaactggt
    23041 ccattcgcgt ttgagtggtg aaacgctcga acacgcggtg gaagtgagta agacctccat
    23101 cacgaccgtt gcgatgctgg aaatgactca ggctggtcgc gaaatgagcg atgaagagct
    23161 caaagaaaac ccagctgtag agcaagaatg ggacattcag tgggaaatat tccgactttt
    23221 agctgagtgc gaagaacgcg atatcgagct gataaaaggc cttagggcag acctgcgtga
    23281 ggcggatgag agcaatattg gtataatttt tcagcaataa gaccagaaaa cgtgatttaa
    23341 cgcctgattt gtcgtacctg gagtcttccc tttcgccccc cgtctggtct acatttgggg
    23401 ggcgaaaaaa agtggctatc ggtgcgtgta tgcaggagag tgctattctg gcatttccgt
    23461 cgcactcgat gcttagcaag cgataaacac attgtaagga taacttatga acaagactca
    23521 actgattgat gtaattgcag agaaagcaga actgtccaaa acccaggcta aagctgctct
    23581 ggagtccact ctggctgcaa ttactgagtc tctgaaagaa ggcgatgctg tacaactggt
    23641 tggtttcggt accttcaaag tgaaccaccg cgctgagcgt actggccgca acccgcagac
    23701 cggtaaagaa atcaaaattg ccgcagctaa cgtaccggca tttgtttctg gcaaggcact
    23761 gaaagacgca gttaagtaag attgcgtggc agtgaacagt tttaacgaag gggtggtttc
    23821 accccttttg tctttctggc gtcgatcatt gatgctggct ggcgctctgt ttctcactgc
    23881 ttgtagtcac aactcttcac ttcctccctt taccgccagt ggatttgctg aagaccaggg
    23941 cgcggtacgc atctggcgaa aagacagcgg cgataatgtg cattttcttg ccgtgtttag
    24001 cccgtggcgc agtggcgata ccacgacgcg agagtatcgc tggcagggcg ataacctcac
    24061 gctcatcaat atcaatgttt acagcaaacc cccggtgaat attcgcgcgc gttttgacga
    24121 tcgcggtgat ctgagcttta tgcaacgtga atccgatggg gaaaagcagc agctttctaa
    24181 cgaccaaatc gatttatacc gttatcgggc tgatcagatc cgccagatta gcgatgcctt
    24241 acgtcagggg agagtcgtgc tgcgccaggg gcgctggcat gcgatggaac agaccgtgac
    24301 cacctgcgaa gggcaaacca ttaaacctga tttagattcg caggcaatag cgcatatcga
    24361 gcgccgccag agccgctctt ctgttgatgt cagcgtggca tggctggaag cgcccgaagg
    24421 ttcgcaatta ctgttagtgg caaactctga tttctgtcgc tggcaaccca acgagaaaac
    24481 gttctgattt accagtggcc catacccata tgaccgccac caccgcagcc gccgtagccc
    24541 atgcccattc cggctccgcg cggaataccc gcttcagcca tcgcaatatc ccgtttcacc
    24601 cgtaactcat ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg
    24661 ctgctatccg gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt
    24721 tgctgttgca gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc
    24781 tgttgttcgc tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca
    24841 aatgcagatg tcgatcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc
    24901 gtgttccgtt tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg
    24961 tgccaacgat gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac
    25021 tcgcctgctg cgggtagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacag
    25081 cgcagggtaa gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg
    25141 caaagaagaa gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg
    25201 cgatcctccc cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg gtgattcgcg
    25261 attatggccg cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta
    25321 ttcgtgctct cgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc
    25381 agcaggcctt actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg
    25441 atgaacaagg aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt
    25501 ccccgcagga aatgcagcag ttacatccgg gagatgaaga agtgtggcgg cggatcgata
    25561 gcgcagacgg cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg
    25621 gaatgcaccg gatgcgccat atgcagcagt atgccgcgac accacaagca attttcatcg
    25681 ctttcgacgc cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta
    25741 tcctcttcgc cctggcgacg gtcttgctgg caagcgtatt gtcattcttc tggtatcgcc
    25801 gctatctgcg ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag aagctggtgg
    25861 cgctggggca tcttgcggca ggcgttgccc acgaaattcg taacccgctt tcctcgatta
    25921 aagggctggc aaaatacttt gccgagcgcg cgccagcagg gggagaagcg catcaactgg
    25981 cgcaggtgat ggcgaaagaa gctgaccgtt taaaccgcgt ggtaagcgag ctgctggaac
    26041 tggttaagcc aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact
    26101 cattacagct ggtaagccag gatgcaaact gccgggagat ccagttacgc tttaccgcca
    26161 acgacacgtt gccggaaatt caggccgatc cggacaggct gactcaggtc ctgttgaatc
    26221 tttatctcaa tgctattcag gcgattggtc agcatggcgt gattagcgtg acggccagcg
    26281 aaagcggcgc gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcggcagatc
    26341 agcttgaagc catcttcacc ccgtacttca ccaccaaagc cgaaggcacc ggactggggc
    26401 tggcggtcgt gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagcctgg
    26461 agggaaaagg cgcaaggttc accctctggc ttccggtcaa tattacgcgt aaggacccac
    26521 aaggatgacg cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac
    26581 tattttgcag gctttgctgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg
    26641 acaggcgctg gagcaggtgc gggagcgggt ttttgatctt gtgctttgcg atgtgcgaat
    26701 ggcggagatg gacggcattg ccacgctaaa agagatcaaa gcgttaaatc cggcgattcc
    26761 ggtactgatt atgaccgcat actctagcgt cgagacggcg gtagaggcgt tgaaaaccgg
    26821 ggcgctggat tatctcatca agccgctgga tttcgataac ctgcaggcga cgctggaaaa
    26881 ggcgctcgcg catacgcaca ttattgatgc tgaaacgcct gcggtgactg ccagccagtt
    26941 cggtatggtc ggtaaaagcc cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc
    27001 gccatcggaa gccacggtgc tgatccacgg cgattccggc accggtaaag agctggtcgc
    27061 cagggcgatt cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc
    27121 ggcactcaac gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac
    27181 tggagccgat aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgttgtttct
    27241 cgatgaaatt ggcgatatct cgccgatgat gctggtgcgt ctgctgcgtg cgattcagga
    27301 gcgcgaagtt cagcgtgtcg gtagcaacca gactatttcg gttgatgtcc ggctgattgc
    27361 cgcgacccat cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta
    27421 ctatcgcctg aacgtggtgg cgattgaagt accatcgctg cggcaacggc gggaagatat
    27481 tcctctgctg gctggccatt ttctgcagcg ctttgctgaa cgtaatcgca aggcggtaaa
    27541 aggctttacg ccccaggcga tggatctgtt gattcattac gactggccgg gaaatattcg
    27601 tgaactggaa aatgcggtgg aacgggcggt ggtgctgctg actggggaat atatttccga
    27661 acgcgagctg ccgctggcga ttgccagtac gccgatcccg ctggcacaaa gcctggatat
    27721 tcagccgctg gtggaagtcg aaaaagaagt gattctggcg gcactggaga aaacgggcgg
    27781 caacaaaacc gaagccgccc gtcagttagg gatcacgcgc aaaacgctgt tggcaaaatt
    27841 gtcgcgttag ttctgctcgc gttcgatagc gcgccagccg atatctttcc ggcagaagca
    27901 gtcgtcccag tggatatcgg tcattaaggc ataggcgcgt ttctgtgctt ctgctacggt
    27961 atgacccagc gcggtgacgc acagtacgcg cccgccgctg gttaccacct gctcgtcatc
    28021 cgccagtttt gtgcccgcgt ggaacacttt gccgtcttcc acttcttcca gcggcaggcc
    28081 gtggatcacg tcaccggtgc ggtaatcgcc cggatatcca cccgcagcca tcaccacgcc
    28141 gagagaagca cgttcatccc actctgacgt tttctcgtct agtttgcctt cacaggccgc
    28201 caggcagagt tcaacaagat cggacttcat gcgcagcata atcggctggg tttctggatc
    28261 gccaaagcgg cagttaaatt cgataacctt cggattgccc tgtttgtcga tcatcaggcc
    28321 cgcgtagaga aaaccggtgt aggtgttgcc ttccgacgcc atgcctttca cggttggcca
    28381 gatgatacgt tccatcgtgc gctgatgaac gtcatctgtc actaccggcg caggagagta
    28441 agcgcccatt ccgcccgtgt tcggcccggt atctttatcg cctacgcgtt tgtgatcctg
    28501 gctggtggcc atcggcagca catgctcgcc gtccaccatc acgataaagc tcgcttcttc
    28561 gccgtcgagg aactcttcga taacgatgcg atgacccgcg tcgccaaacg cgttgcccgc
    28621 cagcatatcg tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt
    28681 cccggcagcc agaccgtccg ctttaatgac gattggcgcg cctttctcac gcagatacgc
    28741 cagcgcaggt tctacctcgg tgaagttctg atattccgcc gtaggaatgt tatggcgggc
    28801 caggaaatct ttagtaaacg ctttcgagcc ttccagctgt gcggcgcctg cggttgggcc
    28861 gaagattttc atcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc
    28921 cgggccgacg atggttagat ctaccttttc gttttgcgcg aaatccagca gcgccgggat
    28981 atcggtcacg ccgatagcga cgttttgcag cgtgggttcc agcgctgtgc ctgcgttacc
    29041 cggagcaaca aaaacagtct caaccagcgg cgactgggcc gctttccagg ccagcgcgtg
    29101 ctcgcgcccg ccgttaccaa tcactaatac tttcatctgt cgctccatta atggcggaag
    29161 tggcgcatat cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg
    29221 tcatcacgga tggaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca
    29281 tcaataccgt cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc
    29341 aggccttcat cggcagcttt aataccggcg attttcgcgg agtacacgcg gctcatctgg
    29401 cccgcgccaa taccgatggt catattgttt ttggcataga cgatagcatt ggatttcacg
    29461 aacttcgcca ctttccagca gaacagcgca tcacgcagtt cctgttcggt cggctgacgt
    29521 ttggtgacga cgcgcagttc ttccgcaccg accatgccca gatcgcgatc ctgaaccagc
    29581 agaccgccgt tcacgcgttt gaaatcaaga cccggaacac gctcgcccca ctgaccgcag
    29641 gtcagaacgc gtacattctg tttggcggcg gtgattttca gggcttcttc gctggcggac
    29701 ggcgcaataa tcacttcaac aaactgacga gaaatgatgg cctgtgcggt ttccgcatcc
    29761 agctcgcggt taaaggcaat gatgccgccg aatgcggagg tcgggtcggt tttgtacgcg
    29821 cgatcgtaag catcaagaat tgaattgccg atagccacgc cgcaagggtt ggcgtgcttc
    29881 acaatcacac atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg
    29941 gcgatgttgt tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct
    30001 tctttcacat tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc
    30061 atatcctgct tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc ggcggcttct
    30121 ttgctttcac cgtgataagc cggaaccatg ctgccgaagt agttggcaat catgctgtcg
    30181 taggcggcag tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc
    30241 gatccttcgt tgtcatccat ctctttaata atggcgtcgt agtcgctgct cttcaccacg
    30301 attgcgacat ctttatggtt cttggcggcg gagcgcacca tcgttgggcc gccgatatcg
    30361 atgttctcaa ccgcatcttc cagcgagcaa ccttcacggg ccacggtctg ggcgaacgga
    30421 tacaggttaa caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg
    30481 tcctggccgc gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt
    30541 ccatccatca tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt
    30601 tctgctaaca gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt
    30661 gcctgggcga attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact
    30721 ggacgacgtt gttgcatggt aaatcccctg gatttgacta ttacagagag cgttagctga
    30781 atttttcgcg aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat
    30841 tgtaacgaaa acgtttgcgc aacgctcgcg aattttttta attcgatggc gatcacgatt
    30901 ttgcccccga ttagcgtggg agaaatagag aaataacgct gatgattgtg gataactctg
    30961 tgcgtaaaaa ggtataaagc ggggttttgc tggggaatgc agcagtcagt catttttctg
    31021 caatttttct attgcggcct gcggagaact ccctataatg cgcctccatc gacacggcgg
    31081 atgtgaatca cttcacacaa acagccggtt cggttgaaga gaaaaat
//
LOCUS       DABSYT020000050        30491 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.050, whole genome shotgun sequence.
ACCESSION   DABSYT020000050 DABSYT020000000
VERSION     DABSYT020000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.050"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(399..2228)
                     /gene="glmS"
                     /locus_tag="ID725_RS13515"
                     /old_locus_tag="pgaptmp_002703"
     CDS             complement(399..2228)
                     /gene="glmS"
                     /locus_tag="ID725_RS13515"
                     /old_locus_tag="pgaptmp_002703"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGV7851272.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2390..3760)
                     /gene="glmU"
                     /locus_tag="ID725_RS13520"
                     /old_locus_tag="pgaptmp_002704"
     CDS             complement(2390..3760)
                     /gene="glmU"
                     /locus_tag="ID725_RS13520"
                     /old_locus_tag="pgaptmp_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGV7851273.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGSGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(4112..4531)
                     /gene="atpC"
                     /locus_tag="ID725_RS13525"
                     /old_locus_tag="pgaptmp_002705"
     CDS             complement(4112..4531)
                     /gene="atpC"
                     /locus_tag="ID725_RS13525"
                     /old_locus_tag="pgaptmp_002705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGV7851274.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4552..5934)
                     /gene="atpD"
                     /locus_tag="ID725_RS13530"
                     /old_locus_tag="pgaptmp_002706"
     CDS             complement(4552..5934)
                     /gene="atpD"
                     /locus_tag="ID725_RS13530"
                     /old_locus_tag="pgaptmp_002706"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGV7851275.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5961..6824)
                     /gene="atpG"
                     /locus_tag="ID725_RS13535"
                     /old_locus_tag="pgaptmp_002707"
     CDS             complement(5961..6824)
                     /gene="atpG"
                     /locus_tag="ID725_RS13535"
                     /old_locus_tag="pgaptmp_002707"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGV7851276.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6875..8416)
                     /gene="atpA"
                     /locus_tag="ID725_RS13540"
                     /old_locus_tag="pgaptmp_002708"
     CDS             complement(6875..8416)
                     /gene="atpA"
                     /locus_tag="ID725_RS13540"
                     /old_locus_tag="pgaptmp_002708"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGV7851277.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8429..8962)
                     /gene="atpH"
                     /locus_tag="ID725_RS13545"
                     /old_locus_tag="pgaptmp_002709"
     CDS             complement(8429..8962)
                     /gene="atpH"
                     /locus_tag="ID725_RS13545"
                     /old_locus_tag="pgaptmp_002709"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGV7851278.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8977..9447)
                     /gene="atpF"
                     /locus_tag="ID725_RS13550"
                     /old_locus_tag="pgaptmp_002710"
     CDS             complement(8977..9447)
                     /gene="atpF"
                     /locus_tag="ID725_RS13550"
                     /old_locus_tag="pgaptmp_002710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGV7851279.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9509..9748)
                     /gene="atpE"
                     /locus_tag="ID725_RS13555"
                     /old_locus_tag="pgaptmp_002711"
     CDS             complement(9509..9748)
                     /gene="atpE"
                     /locus_tag="ID725_RS13555"
                     /old_locus_tag="pgaptmp_002711"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGV7851280.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9795..10610)
                     /gene="atpB"
                     /locus_tag="ID725_RS13560"
                     /old_locus_tag="pgaptmp_002712"
     CDS             complement(9795..10610)
                     /gene="atpB"
                     /locus_tag="ID725_RS13560"
                     /old_locus_tag="pgaptmp_002712"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGV7851281.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10619..10999)
                     /gene="atpI"
                     /locus_tag="ID725_RS13565"
                     /old_locus_tag="pgaptmp_002713"
     CDS             complement(10619..10999)
                     /gene="atpI"
                     /locus_tag="ID725_RS13565"
                     /old_locus_tag="pgaptmp_002713"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGV7851282.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11616..12239)
                     /gene="rsmG"
                     /locus_tag="ID725_RS13570"
                     /old_locus_tag="pgaptmp_002714"
     CDS             complement(11616..12239)
                     /gene="rsmG"
                     /locus_tag="ID725_RS13570"
                     /old_locus_tag="pgaptmp_002714"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGV7851283.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12303..14192)
                     /gene="mnmG"
                     /locus_tag="ID725_RS13575"
                     /old_locus_tag="pgaptmp_002715"
     CDS             complement(12303..14192)
                     /gene="mnmG"
                     /locus_tag="ID725_RS13575"
                     /old_locus_tag="pgaptmp_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGV7851284.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14571..15014)
                     /gene="mioC"
                     /locus_tag="ID725_RS13580"
                     /old_locus_tag="pgaptmp_002716"
     CDS             complement(14571..15014)
                     /gene="mioC"
                     /locus_tag="ID725_RS13580"
                     /old_locus_tag="pgaptmp_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGV7851285.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(15104..15562)
                     /gene="asnC"
                     /locus_tag="ID725_RS13585"
                     /old_locus_tag="pgaptmp_002717"
     CDS             complement(15104..15562)
                     /gene="asnC"
                     /locus_tag="ID725_RS13585"
                     /old_locus_tag="pgaptmp_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGV7851286.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15714..16706
                     /gene="asnA"
                     /locus_tag="ID725_RS13590"
                     /old_locus_tag="pgaptmp_002718"
     CDS             15714..16706
                     /gene="asnA"
                     /locus_tag="ID725_RS13590"
                     /old_locus_tag="pgaptmp_002718"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGV7851287.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16711..18162)
                     /gene="viaA"
                     /locus_tag="ID725_RS13595"
                     /old_locus_tag="pgaptmp_002719"
     CDS             complement(16711..18162)
                     /gene="viaA"
                     /locus_tag="ID725_RS13595"
                     /old_locus_tag="pgaptmp_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGV7851288.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(18156..19652)
                     /gene="ravA"
                     /locus_tag="ID725_RS13600"
                     /old_locus_tag="pgaptmp_002720"
     CDS             complement(18156..19652)
                     /gene="ravA"
                     /locus_tag="ID725_RS13600"
                     /old_locus_tag="pgaptmp_002720"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGV7851289.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19875..21743
                     /gene="kup"
                     /locus_tag="ID725_RS13605"
                     /old_locus_tag="pgaptmp_002721"
     CDS             19875..21743
                     /gene="kup"
                     /locus_tag="ID725_RS13605"
                     /old_locus_tag="pgaptmp_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGV7851290.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21910..22329
                     /gene="rbsD"
                     /locus_tag="ID725_RS13610"
                     /old_locus_tag="pgaptmp_002722"
     CDS             21910..22329
                     /gene="rbsD"
                     /locus_tag="ID725_RS13610"
                     /old_locus_tag="pgaptmp_002722"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGV7851291.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22337..23842
                     /gene="rbsA"
                     /locus_tag="ID725_RS13615"
                     /old_locus_tag="pgaptmp_002723"
     CDS             22337..23842
                     /gene="rbsA"
                     /locus_tag="ID725_RS13615"
                     /old_locus_tag="pgaptmp_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGV7851292.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23847..24812
                     /gene="rbsC"
                     /locus_tag="ID725_RS13620"
                     /old_locus_tag="pgaptmp_002724"
     CDS             23847..24812
                     /gene="rbsC"
                     /locus_tag="ID725_RS13620"
                     /old_locus_tag="pgaptmp_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGV7851293.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24837..25727
                     /gene="rbsB"
                     /locus_tag="ID725_RS13625"
                     /old_locus_tag="pgaptmp_002725"
     CDS             24837..25727
                     /gene="rbsB"
                     /locus_tag="ID725_RS13625"
                     /old_locus_tag="pgaptmp_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGV7851294.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25853..26782
                     /gene="rbsK"
                     /locus_tag="ID725_RS13630"
                     /old_locus_tag="pgaptmp_002726"
     CDS             25853..26782
                     /gene="rbsK"
                     /locus_tag="ID725_RS13630"
                     /old_locus_tag="pgaptmp_002726"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGV7851295.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26786..27778
                     /gene="rbsR"
                     /locus_tag="ID725_RS13635"
                     /old_locus_tag="pgaptmp_002727"
     CDS             26786..27778
                     /gene="rbsR"
                     /locus_tag="ID725_RS13635"
                     /old_locus_tag="pgaptmp_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGV7851296.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27744..29171)
                     /gene="mdtD"
                     /locus_tag="ID725_RS13640"
                     /old_locus_tag="pgaptmp_002728"
     CDS             complement(27744..29171)
                     /gene="mdtD"
                     /locus_tag="ID725_RS13640"
                     /old_locus_tag="pgaptmp_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGV7851297.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(29194..29886)
                     /gene="yieP"
                     /locus_tag="ID725_RS13645"
                     /old_locus_tag="pgaptmp_002729"
     CDS             complement(29194..29886)
                     /gene="yieP"
                     /locus_tag="ID725_RS13645"
                     /old_locus_tag="pgaptmp_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGV7851298.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            30367..>30491
                     /locus_tag="ID725_RS13650"
                     /old_locus_tag="pgaptmp_002730"
     rRNA            30367..>30491
                     /locus_tag="ID725_RS13650"
                     /old_locus_tag="pgaptmp_002730"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7403 a         7767 c         7755 g         7566 t
ORIGIN      
        1 taatccacaa ctatgaattc gcgtagaaaa cagtcttatt tttagtaaac cacactttca
       61 aattatattt tttgcctata tttgtggaaa gactatacat ttacagtgat aatttattat
      121 cacacattgt tacatagtta aataaaatta catattgatg attgttttac aagcgattct
      181 tgaatgaaca aaacgtgcta ttcattacaa agtacatcaa ttatggcaat tgtttgtcaa
      241 tgattcgagg tatttaatta ctactacggt taaaacaagt gttcaacggt gccccaaata
      301 atagctgaag ttaattatat ggaatgaggt tagattatta atgctggtaa gtaaaagggc
      361 cgccaaaagg cagcccttcg ttcagcaaat gaataatatt actcaaccgt taccgatttt
      421 gccaggttac gcggctggtc aacgtcggtg cctttgatca gcgcgacgtg ataagccagt
      481 aactgcagcg gaacggtgta gaagatcggt gcaatcacct cttccacatg cggcatctcg
      541 atgatgtgca tgttatcgct acttacaaaa cccgcatcct gctcggcgaa aacatacaat
      601 tggccgccac gcgcgcgaac ttcttcaatg ttggatttca gtttttccag caattcgttg
      661 ttcggcgcaa cgacgataac cggcatatcg gcgtcaatca gcgccagcgg accgtgtttc
      721 agttcgcctg cagcgtaggc ttcagcgtga atataagaga tctctttcag cttcaatgcg
      781 ccttccagcg cgattgggta ctgatcgcca cggcccagga acagcgcgtg atgtttgtca
      841 gagaaatctt ctgccagagc ttcaatgcgt ttgtcctgag acagcatctg ctcaatacgg
      901 ctcggcagcg cctgcagacc atgcacaatg tcatgttcaa tggaggcatc cagacctttc
      961 aggcgagaca gcttcgccac cagcatcaac agcacagtta actgagtggt gaatgcttta
     1021 gtggatgcca cgccgatttc cgtacccgcg ttggtcatca gcgccagatc ggattcgcgc
     1081 accagagaag aacccggaac gttacagatt gccagcgaac caaggtaacc cagctctttc
     1141 gacagacgca ggcccgccag ggtatccgcg gtttcgccag actgtgacaa ggtgatcatc
     1201 aggctgttac gacgcacggc agatttacga tagcggaatt cagaggcgat ttcgacgtcg
     1261 cacggaatac ctgccagcga ttcaaaccag taacgggaaa ccataccgga gttataagaa
     1321 gtaccacagg cgaggatctg aatatgctca accttcgaca gcagttcgtc ggcgttcggt
     1381 cccagctcgc ttaaatcaac ctgaccgtgg ctgatgcgcc cggtaagggt gtttttgatc
     1441 gcgttcggct gttcgtagat ctctttctgc atgtagtgac ggtaaatacc tttatcgccc
     1501 gcgtcatatt gcagattgga ttcgatatcc tgacgtttta cttccgcgcc agttttatcg
     1561 aagatgttta ccgaacggcg agtgatttcc gcaatatcgc cctcttcaag gaagataaag
     1621 cgacgggtca ccggcagcag cgccagctgg tcagaagcga taaagttttc acccataccc
     1681 agaccaatca ccagcggact accagaacgt gctgccagca gggtatccgg gtgacgggag
     1741 tccatgatca ctgtaccgta cgcaccacgc agttgcggga tagcacgcag aacagcctca
     1801 cgcagagtac cgccctgttt cagctcccag ttcaccagat gggcaatcac ttcggtgtcg
     1861 gtttcagaaa cgaaggtata gccacgcgct tttaactctt cgcgcagcgg ttcatggttt
     1921 tcgatgatgc cgttatgcac caccacaatg tgttcagaaa catgcggatg cgcattcact
     1981 tctgaaggtt caccgtgggt cgcccagcga gtgtgagcaa taccggtgcc gccatgcaga
     2041 ggatgttctt ccgctgcctg agccagcatc tggactttac cgaggcgacg caggcgggtc
     2101 atatgacctt ccgcatcaac aacggccaga ccggcagagt catatccgcg gtattccaga
     2161 cgacgtaaac cttcaagaag gatttctgct acatcacgtt gcgcgatcgc gccaacaatt
     2221 ccacacatag tttttgattc cgatttatat cgttgtcggt caacctgtat gcccgttttt
     2281 tcgggcgccc cgagccttgt agagagtggg gttattttta tagttactgc ttgtgggagg
     2341 gagattatgt tatctcctca tcccatgtga ccggattacc cggccagaat cactttttct
     2401 ttaccggacg acgccagcct tctttctgag tctgcggcac acggctgata gctaatgcat
     2461 tttcgccgac attacgcgtc acagttgtac ccgcagcaat ggtcgcgcct ttgcctactg
     2521 ttaccggggc caccagctga gtgtcggaac cgacaaacac atcgtcgccg ataatggtct
     2581 taaatttatt cgcaccatcg tagttgcagg taatggttcc cgcgccgatg ttaacgttat
     2641 cgccaatttc cgcatcgcca aggtaagtca gatgaccagc tttcgagcct ttacccagac
     2701 gtgctttttt catctcaaca aagttaccga cgtgtgcacc ttccaacaac tcagcaccag
     2761 gacgcaaacg ggcaaacggg ccaatggtac aggccgccgc cagattcgcg tcttccacga
     2821 cggtatatgg gctaatttcg caatcatcgc caatcacgct gtttttaatc acgcaaccgg
     2881 agccgatttt cacgcgatgg ccgagagtca cgttgccctc gatgataacg ttagtatcaa
     2941 tttcaacatc gcgcccgtga gtaagcgtac cgcgcagatc aaaacgcgcc ggatcgcgca
     3001 gcataacgcc tgctaacagc agtttttcag cctgttcgga ctggtaaacg cgctccagac
     3061 gggagagttg caggcggtta ttcacgcctt ctacttcgct taaacgttgc ggatgaacgg
     3121 cgacgatttc acgcccttcc tgatacgcca gcgcaataat gtcggtgatg tagtattcgc
     3181 cctgcgcatt attgttggtc agcttcgcca gccagcgttt catatctgcg ccgttagcga
     3241 tcagaatgcc ggtgttgatc tcctgaatct gacgctgctc gtcggtggca tctttgtgct
     3301 caacaatgcc ggtaactttg ccgttttcac gggtgatacg tccataaccg gtcggatcat
     3361 ccagtttcac cgtcagcaga ccaatgccac cctgcggttt agcatcacgc agacgctgga
     3421 gtgtttcgac agagatcagc ggcacgtcgc cgtagagcat taaaatgtct tcatcatcgg
     3481 caaagaaagg tgcggcctgc tgcatggcat gacccgtacc cagctgctct gcctgaagca
     3541 cccagttcag gttgtcgtct ttcagcgcct gttttagcag atcgccaccg tgaccgtaca
     3601 ccaggtgaac gtgcgctgcg cctaattcat tcgcagcatc aatgacatgc tgaaccatcg
     3661 ctttcccggc aagggtatgc agcactttcg gaagatcgga atacatgcgc gtgcctttac
     3721 ctgcggcaag gatcactacg ctcatagcat tattcaacat acgcgtcctg actgtaattt
     3781 gagaacgaat ttaaaccgct tcaccttgaa aaaactacat ttttttcatc gtgaaatgga
     3841 cagaggataa attgttcaat aacggattat ccccgctgac gaaagcgcca ttttcgacca
     3901 tcgtcacgcc ttttgtctgc agaaaattaa ggcagtacct tcatttaagc gtcaggtgga
     3961 tgtttttgct cttatttcga tcaatgaata aacgggaaaa cagggggttt tatatcagca
     4021 ggatctatgt gaacgctatt caggacgggt cacacgcgca aaaaaaagcc agcctgtttc
     4081 cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc aactcgataa
     4141 cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa tctacgtcgc
     4201 cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct cgcgcttcgt
     4261 cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt cacgttgcca ggctgcactt
     4321 caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc acgatgcgaa
     4381 tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc cccagttcac
     4441 cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt tgctctgcgc
     4501 tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc gttaaagttt
     4561 tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct gctccggcag
     4621 gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt tcagggagac
     4681 gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg acaggaagcg
     4741 ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt catccatacc
     4801 caggatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact gaacgccacg
     4861 cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc tggtggagtc
     4921 cagcgggtca acggccgggt agatacccag agacgcgatc tgacggctca gtaccacggt
     4981 tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat ccgcaggtac
     5041 gtatactgcc tgtacggagg tgatagaacc ggttttggtg gaggtgatac gttcctgcag
     5101 aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca tacggcccag
     5161 cagtgcggat acttccgtac cggccagggt gtaacgatag atgttgtcaa cgaacagcag
     5221 aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca gagctacgcg
     5281 cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt tatcgataac
     5341 gttggagtcg gtcatttcgt ggtagaagtc gttcccctca cgagtacgtt cacctacgcc
     5401 cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaatgagct ccatcatgtt
     5461 tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct tagcgaacgg
     5521 acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt ttgacagctc
     5581 ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga tctcgccttt
     5641 catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt tacctaccgg
     5701 gacttcgatc gggtgttcga ggtcttttac atccagaccg cgacgcagac cgtcggagga
     5761 acccattgcg atggtacgca cgataccgcc gccgagctgc tgctgaactt ccagcaccag
     5821 acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg catcctgagg
     5881 gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag ccatcttaaa
     5941 tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc ggtgagttcc
     6001 tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt aatcaggctg
     6061 ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc gctggccagg
     6121 ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag ggtatccagc
     6181 aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tatgtttcag atcatcatca
     6241 tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat ggtgttaata
     6301 aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaggc ctgcaacatc
     6361 acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc ggtgacctgg
     6421 gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc gatcattgcg
     6481 aggtcgcatt gaacgccttt gtcggtccag gtcttcattt ccgccagcag ttttttgaac
     6541 aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag gtagcccacg
     6601 cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt ccagattacc gtgtgcaagg
     6661 tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat gcgatcctgc
     6721 gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt ttgcgtgttc
     6781 tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt ctcctcaatg
     6841 ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt gctttgaagg
     6901 aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg gtctggttga
     6961 tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg gcttcgaagc
     7021 tgccgatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg aacagaacca
     7081 gagactgctg cgcaacggac atcggcgcat actgtttctg tttcagcagt tcggtcactt
     7141 tttgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca aactgagaga
     7201 acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac agttttttca
     7261 tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg ttaaccgcag
     7321 gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg gtaatggaga
     7381 ttacgttggt cggaacgaac gcagaaacgt cacccgcttg agtttcgata atcggcagcg
     7441 cggtcagtga accggttttc cctttcactt cacctttggt gaaagcttca acgtattcgg
     7501 cgttaacacg cgcagcacgc tccagcagac gagagtggag gtagaatacg tcgcccggga
     7561 atgcttcacg tcctggcgga cgacggagca gcagcgagat ctgacggtaa gcaacagcct
     7621 gtttagacag atcatcgtaa atgatcagcg catcttcacc gcggtcacgg aagtattcgc
     7681 ccattgcgca accggcatac ggtgccaggt attgcagtgc agcggattca gacgcggttg
     7741 ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt accacgttag
     7801 aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg gaatcgcgct
     7861 ggttgatgat ggcatcgata gccagtgcgg ttttaccggt ctgacggtca ccgatgatca
     7921 attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa ccggtctgta
     7981 ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct acagcagaga
     8041 agccgtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg ttaaccacac
     8101 ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac ttaactttca
     8161 tgccttcggc aaggtcagca tacggaccca taacaaccgc accaacagag tcgcgctcga
     8221 ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc tgcatacaat
     8281 cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta ccttcgttgt
     8341 gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg ctgatttcgg
     8401 tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca aggcgctcaa
     8461 gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata acgcctgcca
     8521 ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt ttttccatcg
     8581 cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc agcggaaatg acgtctacct
     8641 cagcggtagc ctcactcacg gcacgaaggt gaataaactg ctccaaaaca tccgggagcg
     8701 cgttaagacg accattttca gccattaccc gaataaggtt ctgaccgttt tcgtccagtt
     8761 gctcaccaca aactgcgata aacgactcgg caagcgtttc tggcgcaagc gcgccagaga
     8821 gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc atgtcctgcc
     8881 agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg taggggcgag
     8941 ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga caagtttatc
     9001 cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct cggcgccagc
     9061 aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac gctcggcttc
     9121 aatttccgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct cagctttcgc
     9181 ttcgtccaga atctgcgagc ggcgtttgtt cgcctgctcg atgattacct gggcttccgc
     9241 tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt ccttatgtgc
     9301 tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga tggctgccat
     9361 taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg cgatggcctg
     9421 gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta acgttctgat
     9481 attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg tacagaccca
     9541 gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag aactgagtac
     9601 gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta cccccgagga
     9661 tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg gcagccatgt
     9721 acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag ttaaaacgta
     9781 gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat cgtcagaacc
     9841 atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca cggcacattc
     9901 aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat cagctcaccg
     9961 gcatacatgt taccgaacag tcgcaaaccg agtgaaactg gtttggacag caggcttacc
    10021 ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg cagcgtcaac
    10081 tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat caggataaat
    10141 acgcccagtg ccatagacag cgttacgttc acgtccgcag acggaaccac acgcagtgca
    10201 ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag taaatccatc
    10261 aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat cagcttgctt
    10321 ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag ctcaatcgcg
    10381 gtctgaaact tacctggcac accgctggtc gcctttttgg ctacgctacg gaataaaacc
    10441 aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat tgtccagaag
    10501 gtggctgggg ggttttgtgg atccaccagc gagaatgtac gcaggtccag ctgaaggtta
    10561 ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat gatgcctttt
    10621 accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc aaaacccacg
    10681 taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc agtaacacca
    10741 acatcgccag aactttgaaa gcttcaccaa atgcgaatgt ccaggccacc cggcctttcg
    10801 ctggtgtatg cgcctggtga cgccaggcaa atatcataaa caaaacgtta ggcagaaaga
    10861 ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg ctgaacagca
    10921 atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca acgtttcgac
    10981 tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta tgccgcgtgt
    11041 cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa attatacggt
    11101 gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt aaatattttt
    11161 ccagtgcata caattgcgac ttttctacta accctgttcg atcatgaaaa actgtaaata
    11221 acgcgtaaac actggtgata aagcgtgctt cagatcacat attgcgcatg ttcgcgcaca
    11281 gcatatttat ttacttggca aatgatgcct ttgcaagttt atgatatttc agtctaaaaa
    11341 cagatactgt tttaataaat gacatttaca caacaaaaac cacccattga catttttaat
    11401 aatgttttaa cagccaatga tggttcttag cgccgatttt tagcagactg atattttcac
    11461 taatgactta ttttctgctt accaaaaaaa gccacgttat cttgttgatg caaaagagtg
    11521 aacgtggcgt taaatgtaac cagttatatc agtagaaaac ctggttgttg ttaacagtct
    11581 aaccggtcaa ttttttatga tttttttgat aaaaattaaa ttttatttgc tttaatcacc
    11641 accagatgac gttcgccatc cagggctgga acctgaagtt taaccactga ttcgacctga
    11701 tattcttcgg gcaacaaagc gatttcatct tccggcattt gccctttcag cgcgtagaaa
    11761 cggccttgct caccaggaag atggtggcac cagctcacca tatcgttcag agaggcaaaa
    11821 gcgcggctaa ttacgccatc aaatggcggc tctgaaggaa actcttctac cctgctctgt
    11881 actggctcaa tattctccag tttaagctca tgttgcacct gacgaaggaa acgcacgcgt
    11941 ttaccaaggc tatccaacag agtgaaatgg gcttcaggac gcacgataga gagtggaatg
    12001 ccaggcagtc caggaccggt gccgacatcg ataaaccgtt caccttgcag atacggtgcc
    12061 accacaatgc tatcgagaat atggcgtacc agcatctcat taggatcgcg gaccgaagtc
    12121 aggttgtacg ctttgttcca tttatgcagc atattcacgt aggcaataag ctggtttttc
    12181 tggtgatcgg taagcgaaat acctgcgtct ttcagcagta aggagagttt gttgagcacg
    12241 gtgattacct gttcttgatg cgttgcctgg taagcggggg cttaccaggc atttttaatg
    12301 cgttatgcgc tacgacgcag cataccctgt tttttcagcc acaccagcag aatggagatg
    12361 gccgcaggcg tgacgccaga aatacgtgaa gcctggccga tagaggccgg tttgtgatcg
    12421 ttaagtttgg cgatcacttc gttagaaaga ccggatacct ggcggtaatc cagtgtcgcg
    12481 ggtagcaagg tgttctcgtt acgcagctgc ttttcgatct catcttgctg gcgcgcgata
    12541 taaccttcgt atttaacctg aatctcaacc tgttccgccg cctgttcgtc tgtcaacgca
    12601 ggggcaaacg gcgtcagcgt ggttaatttt tcataagtca tttccggacg acgcagcaga
    12661 tcttcaccac tggcttcacg ggaaagcggc gcagtcaggt gagcattcac ttcggctgca
    12721 gcttccgccg acggggttac ccaggtcgat ttcagacgct gacgctcacg ctcgatattc
    12781 tcaagtttct cgttaaagcg cgcccaacgt tcgtcatcca ccaggcccag ttcacgaccg
    12841 atttcagtca aacgcagatc cgcattatct tcgcgcagca tcagacgata ttctgcgcgc
    12901 gaggtaaaca tacgatacgg ttctttggtt cctaaagtgc acaggtcatc aaccagtacg
    12961 ccgagatagg cctgagaacg cgccggagcc caaccttctt tgtcatcaga cagacgggca
    13021 gcgttaagac cggccagcaa accttgcgca gcggcttctt cgtaaccggt agtgccgtta
    13081 atctgaccag caaagaacag cccctggata aacttgctct ccagcgtcgg tttcaggtcg
    13141 cgaggatcga agaagtcata ctcaatggca taacccggac gcacgatctt cgcgttttcc
    13201 atcccctgca tagagcggac gatttgcatc tgcacatcga acggcaggct ggtggagata
    13261 ccgttcggat aaatttcgtt agaagtcagc ccttccggtt caaggaagat ctgatgctga
    13321 tttctgtcgg cgaagcgcat gactttgtct tcgatcgacg ggcagtagcg tgggccgaca
    13381 ccttcgatca cccctgcgta cattgggcta cgatcgaggt tactgcggat cacatcatgg
    13441 gttttctcgt tggtatgggt gatataacac ggcacctgct gtggatgctg ggacgcattg
    13501 cccataaaag agaataccgg cattgggtta tcgccatgct gttgcgcaag cacgctaaag
    13561 tcgatggttc gagcatcaat acgcggtggt gtcccggttt tcagacgacc aacgcgcagc
    13621 ggaagttcac gcaaacggcg agaaagcgga atggacggcg gatcaccagc acggccaccg
    13681 ctgtagttat ccagaccgat atgaatttta ccgtcgagga acgtcccaac ggtgagcacg
    13741 acggctttgg cacggaactt cagtcccatt tgggtaacag caccgaccac acgatcgttt
    13801 tcgacaataa gatcttcaac cgcctgctgg aagatcatca ggttcggttg gttctccagc
    13861 gccgtacgta ccgcctgacg gtagagcaca cgatccgcct gagctcgggt agcgcgaact
    13921 gccggtccct tgcttgcgtt tagtatccta aactggatac ccgcctgatc gatcgctttc
    13981 gccatcagac cgccgagtgc atccacttct tttaccagat gtcccttccc aataccgccg
    14041 atcgccgggt tgcagctcat ctgccccaga gtgtcgatat tgtgtgtcaa aagcagagtc
    14101 tgttgaccca tacgcgccgc agccatcgcg gcctcggtgc ctgcatgacc cccgccaatg
    14161 atgatgacgt caaaaggatc cggataaaac atggtgattg cctcgcataa cgcggtatga
    14221 aaatggattg aagcccgggc cgtggattct actcaacttt gtcggcttga gaaagacctg
    14281 ggatcctggg tattaaaaag aagatctatt tatttagaga tctgttctat tgttatctct
    14341 tattaggatc gcactgccct gtggataaca aggatccggc ttttaagatc aacaacctgg
    14401 aaaggatcat taactgtgaa tgatcggtga tcctggaccg tataagctgg gatcagaatg
    14461 aggggttata cacaactcaa aaactgaaca acggttgttc tttggataac taccggttga
    14521 tccaagcttc ctgacagagt tatccacagt agatcgcacg atctgtatac ttatttgagt
    14581 aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc gtgatcaaga
    14641 atgttgatct tcagtgtttc gcccgtctgt tttgcaccgg aatttttgag ttcagcctcg
    14701 agtttatcga tagccccaca aaatgtgtca tattcacgac taccaatacc gattgcgcca
    14761 aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa aggagaaagg
    14821 ttgtccggaa tatctccggc accgtgggtg gagctgataa ccagccagat ccccgaggca
    14881 gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc ctcttccagc
    14941 ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc gctgataaga
    15001 gttatatctg ccataaaccg ccacctttat taagagtggc gtattgtacg ctgtgaacgc
    15061 gttgggatct acctgtggaa aagtatggga ttaaaaaagc cgatcagggc ttgatggtac
    15121 gcatgatcgg gttctgcagg acgatcagtg tctcggtgga ctgaatttca tcaattgttt
    15181 ggatcttgtt gataagtaca tgctggagag catcgatcga acggcacatc acttttataa
    15241 agatgctgta gtggccggtg gtgtagtacg cctcggtcac ttcatcgagg ctttccagct
    15301 ttgccagcgc ggaagggtag tctttggcgc tctttaatat aatgccgata aagcagccta
    15361 cgtcataacc gagctgcttc gggctgacat caatacgcgc cccggtaatg atccccgcct
    15421 gcttcatttt ctctactcga acgtgaatcg tccccggact gacgccaaat tgtttcgcca
    15481 gttcggcgta agcggtgcgc gcattgccca ttaatgcttc caggatgcca cggtccagat
    15541 tgtcgatcag ataattttcc ataggatttt cttatgcgga ttgatgattc attctatttt
    15601 agccttcttt tttaatgaat caaaagtgag ttaggctttt tattgaatga ttattgcatg
    15661 tgtgtcggtt tttgttgctt aatcataagc aacaggacgc aggagtataa aaaatgaaaa
    15721 ccgcttacat tgccaaacaa cgccaaatta gcttcgtgaa atctcacttt tctcgtcaac
    15781 tggaagaacg tctggggctg atcgaagtcc aggcgccgat tcttagccgt gtgggggatg
    15841 gcacgcagga taacttgtcg ggctgtgaaa aagcggtaca ggtaaaagtg aaagctctgc
    15901 ctgatgccca gttcgaagtg gttcattcac tggcgaagtg gaaacgtcag accttagggc
    15961 aacacgactt cagcgcgggc gaagggctgt acacgcacat gaaagccctt cgccccgatg
    16021 aagaccgtct ttctccgttg cactcggtct atgttgacca gtgggactgg gaacgcgtaa
    16081 tgggcgacgg tgagcgtcaa ttctcgactc tgaaaagcac ggtagaggcg atttgggcgg
    16141 gaattaaagc aaccgaagct gcggttagcg aagagtttgg cctggcaccg ttcctgccgg
    16201 atcagatcca cttcgtacac agccaggagt tactgtctcg ttatccggat cttgatgcca
    16261 aagggcgtga gcgggcgata gcgaaagatc ttggtgcggt attccttgtc gggattggcg
    16321 gcaagctgag cgatggtcat cgccacgacg tgcgcgcacc ggattatgat gactggagca
    16381 ccccgtcaga gctgggccat gcgggtctga acggcgatat tctggtgtgg aaccctgtac
    16441 tggaagatgc gtttgagctt tcttccatgg ggatccgcgt ggatgccgac acgttgaagc
    16501 atcagttggc gctgaccggt gatgaagatc gtctgcagct ggaatggcat caggcgttgc
    16561 tgcgcggtga aatgccgcag accatcggcg gcggtatcgg ccagtctcgt ttgaccatgc
    16621 tgctgctgca actgccgcat atcggccagg tccagtgtgg agtatggcca gctgctgttc
    16681 gcgagagcgt cccttctctg ctgtaataat ttatcgccgc cagcgtctga gcaggcggct
    16741 tcgcatcccg gtatcaaagc gccagatatg atcgaaaatg cgcatgatgc cgggtttgcc
    16801 gtgtgccgac atcgccacgg catgaaagcg atgctgatgt acccgctgca gctctttcac
    16861 tttactcgtc acgtcgtcag gcaaccgctg ggcgataaaa tcagaaatca ccaccgcatc
    16921 ggcatcaaac cattccctgc tttgcaagcg ttccataatg gcgcgaaaac aactggcaag
    16981 atcggtgccg ccacgaaact gctggcttaa aaaacggatt gcttgttcga tgccttgtgg
    17041 gcctgaaagc tcataacgga cgatctcggt ggaaaatagc ataatataac agcgccggtt
    17101 ttcagcgaga gcaatgcgca tcaaagccag gcagaacgct ttcgcacact gttcattaaa
    17161 gccgcccatt gagccggaag tatccacgca gacaataaac ggtccgcgcg gctgttcgtc
    17221 gtaatcttta tgcaccaccg ggcgttcgat cactttttca cgccacgact caccgtgcag
    17281 gcgataggtg agcaactgtt tttccaccag ccgacggtaa aactcatact ccagttccgt
    17341 tatccctagt gtcgccagtt ctggcggcag aagacgtaaa atatcatcgc tttgttgcag
    17401 accatcaacc tgctcaggaa ccgtcgccgg ttcgcgcacc atggtgcgga aggtttccat
    17461 ctgcgcatcg ttgcgcggta ttgatttggc ttcccgggaa cgccccagtt gttctgccag
    17521 gcgtttcagt tccggctgtt cgttaagaaa ttcaccgtat ttcacaatca actgatagtc
    17581 gccacgttta agctgaccgg cgctcatatc ccacagacga ccagctgcgg tattgttatc
    17641 tgcgagaatc ggttcaagtt gtccgctcag cgtcatgcgt tcctgaactt cactcaacag
    17701 ttgttcgcgt tcttcttcta atagctgttg atttaacgtt gttgcttgca cgatcagact
    17761 taaacgccag cgctggagaa aaagcgtgtg taacgctgaa gtgatcgtgc tgttagcatc
    17821 gaccaactgt cgggcttgtt ctgcccatgg ggaattcaga cgatgcagta agtccaggat
    17881 ctgtggtagc tgcacaataa actgcggcgt ggagaggagc tggctttgct gatagcacat
    17941 cacctcttcg gtgagttccg gcgggactcg ggcatctttc agccgactgc gcagcgcctc
    18001 acgccagcgg ggaacatcat cagtgattgc cgccttcagt cgtgggaatt tttcaaagaa
    18061 gactgccagc tgcggtgagg ccagcagcgc gatgatcatc tcttcgatca atccctcttc
    18121 gctgacggcc agcatcacat taagcgtatc cagcgttagc attgttgtgc ctggcgaatc
    18181 tgtgcgccga catcctgtag gctggcttca atgcgaccta accagtcgcc agggataaac
    18241 agacatcttt gctgttcgct gaataaagcg tgttgcttgc gccagtcgct ttcaagctct
    18301 tccaattgtt gttttatttc acctggcaga ccttcagccg atgaaccggg aagtgccagc
    18361 gtactgcctt gtaaacttac atcgcgtaca acaagatgtt gggcgctatc aacttccaga
    18421 ttcagtttct gggcaaagcc gataccgttc agtttgccgc gaatttcacc acctttgctc
    18481 agccactgtt ccagcgcgct acgctcaaag gagatatgaa ccacttccat attatgcaat
    18541 tttaacggtt tttgcagcag cagagtcaga gtggaagcag taacgttaac aggaagttga
    18601 tactgctgac gacggctgaa aatgccgccc agacgaatta ccgttaaggc tgttttatcg
    18661 ctttgttgct gctggagttg caggtgacgt tgcacaatcg cgcccaggcg ggtcaacatc
    18721 ccttgctgtt gccaggcatg accggtcatc aatacatcaa tttgttgttg tatcaaattc
    18781 aggctttgcg cgtcatacca caggcaatct ttcagcaaaa tgagatctac cggggcaaca
    18841 gcactgcgac cgctaaaaaa ggcgctggcc tgcaataatc gaatcgcttt tttccagcga
    18901 cgatccgaga cataaggcgc atccggtaat ttatccagtt gctggcgcag cataaaaatc
    18961 agctcaaata catgatcggg cagcgtaatt tcaccaatct ctttttgcca gcgttcatat
    19021 tcttcatctg tgacctgcaa ggaggctgga accggattgt cattttcatc ctgttgactg
    19081 gtcagcatgg agcggaaatt cgctttatcc tgcactttat ctaaccacag acgaatcagc
    19141 atgcggtcat ataacgcttc cagactgctg tctgcttccg gcagctcgtt ggaggccgcc
    19201 accagcaggc gcatcgggat tttttctacg tgtgcaccgt tgcggaactg gcgctcgtta
    19261 atggcggtga gcagggtatt aagaattgcc ggacccgctt tccagatctc atccagaaag
    19321 acgatttctg cttccggcag gtaaccgctg gttaaacgtt catagcgccc ttcatctttt
    19381 agcgcctgaa tagaaagggg accaaaaact tcttccggcg tggagaagcg ggtcatcaga
    19441 tattcaaacg cgcgggcatt ctgaaaggcg aattttaagc gccgggcgat caaacttttg
    19501 gcaatacctg gcgggccaag gaggaacaca ctttcaccac ttaatgccgc taataaacac
    19561 aagcggatgg cgtggctacg ttcataaagc cccttttcca acgaactgct caggcgggaa
    19621 attctttccg ctaataaatg agggtgagcc ataatgaagt ggcgtccttt cgtcaaaagt
    19681 tctgcgtaaa ttgcgagtat agacgtttct tgctggtggc taaaatagtc tcaaaggggg
    19741 ggtatttttc tttgagccag gttaatgtgg ccgcatttag gagtacgatt ttgccgttaa
    19801 tcgtgcatac tgtgcgcttt tttgtgggcc aagggactaa gcacacattt catatttcaa
    19861 cgaaagacta gtctatgagc actgataata agcaatcatt gcccgcgatt accctcgcgg
    19921 cgattggagt tgtctacggc gatattggta ccagcccgtt atatacactt cgtgaatgtt
    19981 tgtccggcca gtttggtttt ggcgttgaac gcgatgccgt gtttggcttt ttatcgctga
    20041 tcttctggct gctaatcttt gtggtttcca ttaaatatct caccttcgtg atgcgggcag
    20101 ataacgccgg tgaagggggg atcctgacgt tgatgtcgct tgccgggcgt aatacgtcgg
    20161 cgcgaaccac atcaatgctg gtgattatgg ggctaatcgg cggcagcttt ttctatggtg
    20221 aagtcgtcat aacacccgct atttcggtga tgtcagccat tgaaggtctg gaaatcgtcg
    20281 ccccgcagct ggatacctgg atagttcccc tctcaattat cgttctcact ttattattta
    20341 tgattcaaaa gcatggcacg gctatggtcg gtaagctgtt tgcgccgatc atgctgacct
    20401 ggtttttgat tctggcagga ctggggttac gtagcattat tgctaaccag gaagtgctgt
    20461 atgcgctgaa tccgatgtgg gcggtgcatt tcttccttga atacaaaacg gtttctttta
    20521 ttgcattagg ggcagtggtg ctgtcgatta cggggggcga ggcgctgtat gctgatatgg
    20581 ggcactttgg taagttccct attcgcctgg cgtggttcac cgtcgtatta ccttccttga
    20641 cccttaatta cttcggtcag ggagcgctgt tgttaaagaa tccggaagcg attaagaacc
    20701 cgttcttcct tttggcaccg gactgggcgc tgatcccgct gctgatcatc gccgcactgg
    20761 cgacggtaat cgcctcgcag gcggttatct ctggcgtctt ttcattgacg cgtcaggcgg
    20821 tacgtctggg atatttgtcg ccgatgcgca ttattcacac ctccgaaatg gagtcagggc
    20881 aaatctatat tccgtttgtg aactggatgc tctatgtcgc ggtcgtgatt gtgattgtca
    20941 gctttgagca ctccagcaac ctggcggcgg cgtacgggat tgcggtgacc ggaaccatgg
    21001 tgctgacgtc tattctctcg actaccgtgg cacgtcagaa ctggcactgg aataagtatt
    21061 ttgttgcgct gatcttgatt gctttccttt gtgtcgatat tccattgttc accgctaacc
    21121 tcgataaact gctctccggc ggctggttgc cattgagcct cggtacggtg atgtttatcg
    21181 tgatgaccac ctggaagagt gagcgtttcc gcttgctgcg gcggatgcat gaacatggta
    21241 actctctgga agcgatgatt gcttcgctgg agaaatcacc gcctgttcgc gtgcccggga
    21301 ccgcggtata tatgtcgcgt gcaatcaacg tcattccctt tgcgctgatg cataacctta
    21361 aacataacaa ggtattgcat gagcgggtga ttctgttaac tctgcgcacc gaagacgctc
    21421 catatgtcca taacgtccgt cgggtacaga ttgaacaact gtcgcccact ttctggcgcg
    21481 tggtggcaag ttatggttgg cgagaaacgc caaacgtgga agaagttttc caccgctgcg
    21541 gtctggaagg attaagttgc cggatgatgg aaacctcctt ctttatgtcg catgagtcgt
    21601 tgatcctcgg caaacgcccg tggtatttgc gtctgcgcgg caagctgtac ttgctgctgc
    21661 aacgtaatgc gctgcgtgca ccagatcaat ttgaaatccc gccaaacagg gttatcgaac
    21721 tgggtactca ggtcgaaatc taacgccaga cgcctccttt cttcataagg gggcgttttt
    21781 gttttcatgg ttaatcacca tgtaaaacgt ttcgaggttg atcacatttc cgtaacgtca
    21841 cgatggtttt cccaactcag tcaggattaa actgcgggtc agcgaaacgt ttcgctgatg
    21901 gagaaaaaaa tgaaaaaagg caccgttctt aattctgata tttcatcggt gatctcccgt
    21961 ctgggacata ccgatacgct ggtggtgtgt gatgctggtt tacccatccc caaaagtaca
    22021 acgcgtatcg atatggcatt aacccagggt gtaccttctt ttatgcaggt gctgggcgtc
    22081 gtcacaaatg aaatgcaggt cgaggctgcc attatcgcgg aagagatcaa acaccataat
    22141 ccgcaactcc acgaaacgtt gctcactcac cttgagcagc tgcaaaaaca ccagggaaat
    22201 accattgaaa ttcgttacac cacgcatgaa caattcaaac aacaaaccgc agaaagtcag
    22261 gcggtaattc gcagcggaga atgttctccg tatgcgaata tcattctctg tgctggcgtg
    22321 acgttctgag gccgtcatgg aagcattact tcagcttaaa ggcatcgata aagccttccc
    22381 gggcgtaaaa gccctctcgg gcgcagcgtt aaatgtctat ccgggccgcg tgatggcgct
    22441 ggtgggcgaa aacggcgcgg gtaaatccac catgatgaaa gtgcttactg gcatctatac
    22501 tcgcgatgcc ggtacgcttt tatggctggg gaaagaaacg acatttaccg ggccgaaatc
    22561 ttcccaggaa gccgggattg ggattatcca tcaggaactg aacctgatcc cgcagttaac
    22621 cattgccgaa aacattttcc tcggtcgtga gtttgttaat cgctttggca aaattgactg
    22681 gaaaaccatg tatgccgaag cggataaatt gctggctaaa cttaacctgc gctttaaaag
    22741 cgacaagctg gtgggcgatc tttccatcgg tgaccagcaa atggttgaaa tagccaaagt
    22801 gctgagcttt gagtcgaaag tcatcattat ggatgaaccg accgatgcgc tgaccgatac
    22861 cgaaaccgaa tccctgttcc gcgtcatccg cgagctgaaa tcgcaaggcc gcggtattgt
    22921 ctatatctcc caccgcatga aagaaatctt cgagagttgc gatgacgtta ccgtttttcg
    22981 tgacggacaa tttattgctg agcgcgaagt ggcatcactg accgaagatt cgctgattga
    23041 gatgatggtg ggtcgcaagc tggaagatca atatccgcac ctggacaaag cgccgggaga
    23101 tatccgcctg aaagtcgata atctctgcgg acctggcgtt aacgatgtct cttttacttt
    23161 acgcaaaggc gaaattcttg gcgtctctgg tttgatgggc gcaggtcgta ccgaactgat
    23221 gaaagtgctc tacggcgcac taccgcgcac cagcggttac gtcaccctgg atgggcatga
    23281 agtcgttacc cgttcaccgc aggatggcct ggcaaacggc attgtgtata tctccgaaga
    23341 ccgtaaacgt gacggtttag tgttgggcat gtcagtaaaa gagaacatgt cgctgacagc
    23401 gctgcgctac ttcagccgcg ctggcggcag tttgaagcat gccgatgaac agcaggctgt
    23461 gagtgatttt attcgcctgt tcaatgtgaa aacgccgtcg atggagcagg caattggtct
    23521 gctttccggt ggcaatcagc aaaaagtggc gattgcccgc ggtctgatga cacgccccaa
    23581 agtgttgatc cttgatgaac ctacccgtgg cgtagatgtc ggcgcgaaaa aagagatcta
    23641 tcaactgatt aaccagttca aagccgatgg cttgagcatc attctggtgt catcggagat
    23701 gccagaagta ttaggcatga gcgatcgcat catcgtcatg catgaagggc atctcagcgg
    23761 ggaatttact cgtgagcagg ccacccagga agtgttaatg gctgccgctg tgggcaagct
    23821 taatcgcgtg aatcaggagt aaaaaaatga caacccagac tgtctctggt cgccgttatt
    23881 tcacgaaagc gtggctgatg gagcagaaat cgcttatcgc tctgctggtg ctgatcgcga
    23941 ttgtctcgac gttaagcccg aactttttca ccatcaataa cttattcaat attctccagc
    24001 aaacctcagt gaacgccatt atggcggtcg ggatgacgct ggtgatcctg acgtcgggca
    24061 tcgacttgtc ggtaggttct ctgttggcgc taaccggcgc agttgctgca tctatcgtcg
    24121 gcattgaagt caatgcgctg gtggctgtcg ctgccgctct cgcgttaggt gccgcaattg
    24181 gtgcggtaac cggagtgatt gtagcgaaag gtcgcgtcca ggcgtttatc gctacgctgg
    24241 ttatgatgct tttactgcgc ggcgtgacca tggtttatac caacggtagc ccagtgaata
    24301 ccggctttac tgagaacgcc gatctgtttg gctggtttgg tattggtcgt ccgctgggcg
    24361 taccgacgcc agtctggatc atggggattg tcttcctcgc ggcctggtac atgctgcatc
    24421 acacgcgtct ggggcgttac atctacgcgc tgggcggcaa cgaagcggca acgcgtcttt
    24481 ctggtatcaa cgtcaataaa atcaaaatca tcgtctattc tctttgtggt ctgctggcat
    24541 cactggccgg gatcattgaa gtggcgcgtc tctcctccgc acaacccacg gcggggactg
    24601 gctatgagct ggatgctatt gctgcggtgg ttctgggcgg tacgagtctg gcgggcggaa
    24661 aaggtcgcat tgttgggacg ttgatcggcg cattaattct tggcttcctt aataatggat
    24721 tgaatttgtt aggtgtttcc tcctattacc agatgatcgt caaagcggtg gtgattttgc
    24781 tggcggtgct ggtagacaac aaaaagcagt aataacgact acaggacatc ttgaatatga
    24841 acatgaaaaa actggctacc ctggtttccg ctgttgcgct aagcgccacc gtcagtgcga
    24901 atgcgatggc aaaagacacc atcgcgctgg tggtctccac gcttaacaac ccgttctttg
    24961 tatcgctgaa agatggcgcg cagaaagagg cggataaact tggctataac ctggtggtgc
    25021 tggactccca gaacaacccg gcgaaagagc tggcgaacgt gcaggactta accgttcgcg
    25081 gcaccaaaat tctgctgatt aacccgaccg actccgacgc agtaggtaat gctgtgaaga
    25141 tggctaacca ggcgaacatc ccggttatca ctcttgaccg ccaggcaacg aaaggtgaag
    25201 tggtgagcca cattgcttct gataacgtac tgggtggcaa aatcgccggt gattacatcg
    25261 cgaagaaagc gggtgaaggt gccaaagtta tcgagctgca aggcattgct ggtacatccg
    25321 cagcccgtga acgtggcgaa ggcttccagc aggccgttgc tgctcacaag tttaatgttc
    25381 ttgccagcca gccagcagat tttgatcgca ccaaaggttt gaacgtaatg cagaacctgt
    25441 tgaccgctca tccggatgtt caggctgtat tcgcgcagaa tgatgaaatg gcgctgggcg
    25501 cgctgcgcgc actgcaaact gccggtaaat cggatgtgat ggtcgtcgga tttgacggta
    25561 caccggatgg cgaaaaagcg gtgaatgatg gcaaactagc agcgactatc gctcagctac
    25621 ccgatcagat tggcgcgaaa ggcgttgaaa ccgcagataa agtgctgaaa ggcgagaaag
    25681 ttcaggctaa gtatccggtt gatctgaaac tggttgttaa gcagtagttt taatcaggtt
    25741 gtatgacctg atggtgacat aaatacgtca tcgacagatg aacgtgtaat ataaagaaaa
    25801 gcagggcacg cgccacccta acacggtggc gcattttatg gacatcccga atatgcaaaa
    25861 cgcaggcagc ctcgttgttc ttggcagcat taatgctgac cacattctta atcttcaatc
    25921 ttttcctact ccaggcgaaa ccgtaaccgg taaccactat caggttgcat ttggcggcaa
    25981 aggcgcgaat caggctgtgg ctgctgggcg tagcggtgcg aatatcgcgt ttattgcctg
    26041 tactggcgat gacagcattg gcgagagcgt tcgccagcag ctcgccactg ataacatcga
    26101 tattactccg gtcagcgtga tcaaaggcga atcaacaggt gtggcgctga tttttgttaa
    26161 tggcgaaggt gagaatgtca tcggtattca tgccggcgct aatgctgccc tttccccggc
    26221 gctggtggaa gcgcaacgtg agcgtattgc caacgcgtca gcattattaa tgcagctgga
    26281 atcaccactc gaaagtgtga tggcagcggc gaaaatcgcc catcaaaata agactatcgt
    26341 tgcgcttaac ccggctccgg ctcgcgaact tcctgacgaa ctgctggcgc tggtggacat
    26401 tattacgcca aacgaaacgg aagcagaaaa gctcaccggt attcgtgttg aaaatgatga
    26461 agatgcagcg aaggcggcgc aggtactgca tgaaaaaggt atccgtactg tactgattac
    26521 tttaggaagt cgtggtgtat gggctagcgt gaatggtgaa ggtcagcgcg ttcctggatt
    26581 ccgggtgcag gctgtcgata ccattgctgc cggagatacc tttaacggcg cgttaatcac
    26641 ggcgttgctg gaagaaaaac cattgccaga ggcgattcgg tttgcccatg ctgccgctgc
    26701 gattgccgta acacgtaaag gcgcacaacc ttccgtaccg tggcgtgaag agatcgacgc
    26761 atttttagac aggcagaggt gacgcttggc tacaatgaaa gatgttgccc gcctggcggg
    26821 cgtttctacc tcaacagttt ctcacgttat caataaagat cgcttcgtca gtgaagcgat
    26881 taccgccaaa gttgaagcgg cgattaaaga actcaattac gcgccatcag ctctggcgcg
    26941 tagcctcaaa ctcaatcaaa cacataccat tggcatgttg atcactgcca gtaccaatcc
    27001 tttctattca gaactggtgc gtggcgttga acgcagctgc ttcgaacgcg gttatagtct
    27061 cgtcctttgc aataccgaag gcgatgaaca gcggatgaat cgcaatctgg aaacgctgat
    27121 gcaaaaacgc gttgatggct tgctgttact gtgcaccgaa acgcatcaac cttcgcgtga
    27181 aatcatgcaa cgttatccga cagtgcctac tgtgatgatg gactgggctc cgttcgatgg
    27241 cgacagcgat cttattcagg ataactcgtt gctgggcgga gacttagcaa cgcaatatct
    27301 gatcgataaa ggtcataccc gtatcgcctg tattaccggc ccgctggata aaactccggc
    27361 gcgcctgcgg ttggaaggtt atcgggcggc gatgaaacgt gcgggtctca acattcctga
    27421 tggctatgaa gtcactggtg attttgaatt taacggcggg tttgacgcta tgcgccaact
    27481 gctatcacat ccgctgcgtc ctcaggccgt ctttaccgga aatgacgcta tggctgttgg
    27541 cgtttaccag gcgttatatc aggcagagtt acaggttccg caggatatcg cggtgattgg
    27601 ctatgacgat atcgaactgg caagctttat gacgccacca ttaaccacga tccatcaacc
    27661 aaaagatgaa ctgggggagc tggcgattga tgtactcatc catcggataa cccagccgac
    27721 ccttcagcaa caacgattac aacttactcc gattctgatg gaacgtggtt cggcttagat
    27781 ttacgctgtc ttttgatcaa attattacca tcggttgttt tcagaagcat gaacattgct
    27841 gctgaagcaa cagtaataat gcccattgtg ataaacgtat agtggaattg ttcgacagtc
    27901 gttgtgcctt ccattccttc ataaacgcga aggacggccg cacttacagc tacgcctaaa
    27961 ctgatagaca gttgctgcgt gaccgccaga acactgttac cgctgctggc gttgtcatcg
    28021 gtcagatcgg caagtgtgat ggtattcatc gcggtaaact gcgtcgacat agccatccct
    28081 aatataaaca acggcaagat cagcatccat attgccattg ccggtgattg caaagagaac
    28141 tgagcgatca ttagcccaat aatcaccgtg atccccacta atgtatggcg atagcccaga
    28201 cgacgtaaga cttgggtaac catcgatttt gcaataatgg aacctaacgc tgtcggtgcc
    28261 atcatacagc cagcaataaa cgcctgataa ccaaatccta cctgtaacat taatggcata
    28321 aggaacggta cacagccggt ccccagacgg gttgcaatat tgcctacgat accgatcgag
    28381 aaagtgcggg ttttaaataa atctaatgaa attaatgggt ttggcgtgcg tcgtgcatgg
    28441 agaatataga gaagcagtaa cccgatgctg gtgacaatta ccgtcaaggc aatccagctg
    28501 gcgacaatct tttccccgaa tagctctatt ccgcttgaga agagaacaag gctgaggcca
    28561 aacagcaaaa agccagtgat atcgaatctg cgtcgtgcgg tggtgaaatt gggcatatgt
    28621 ttgcgcgcgt aaagaaggcc cgcaatacct atggggatat tgattaaaaa tatccagtgc
    28681 caggttgccc aggtgaccag cacgccgcca agaatggggc ctaaaattgg ccccaccaga
    28741 cccggcatgg cgacaaaatt caatactgga agaagttcat tacgaggata agcgcgcagt
    28801 aaggccagcc gagcaacagg catcatcatt gcgccgccta tcccctgaat aacccggaag
    28861 acaaccagct gtggtagcga attagaaagt gcgcaggcca gagaacccaa tgtgaacaga
    28921 ctcacggcaa gggtaaaaat gcgacgcgta ccgaagcgat cggctagcca tccgcttacc
    28981 ggaataagca tcgccaccgt cagcgtataa ctgatgatgg ctgattgcat cgcgagagga
    29041 gaacgattaa ggctatgagc gattgcgggt aaggcggtat tcagaatagt ggcatcaagt
    29101 gcctgcatga agaaggccat cgccgcgatc cacggcaaac ccgccatact gcgcttcttt
    29161 ttatcgctca ttcaatgtcc tgttatcggg ttatcactta tcaggtgagc gtagcagcgc
    29221 ctgacaagct ttaaatgccg cgtcgccatc gctttggata atcgcatcga ctatcgcctg
    29281 atgcagatcc agctttatca ctgtgtcgct ggtaattgac gtgaagtaag tgtgataaac
    29341 cgaatggaat agcgaggcga atgaggtcaa aaacggattg gcgctcattt catagatatg
    29401 ctcatgccag gccatatcga cttcgatcca gcgttcacgg cgaaagttct cttttaatgc
    29461 cgccatttcg gccattaacg tattgagatg cgctttctgt tccgcggtgc caaccgttgc
    29521 tgccagtagg caagcttgtg gctccagaca gatacgcata accagaaagt gatcgatgac
    29581 ctgatgaaag ttctcttctg tcatccacca ggtaagcaat tcctgatcaa gaaaattcca
    29641 gttcgattgt ggcatgaccc gggtaccaat tcgcggtcgc ggtaaaacca tcccttttgc
    29701 cgttaacgtt ttgaccgctt cgcgtaccgc tgtacgactc actccaaatt gctcgcccag
    29761 ctcaatctca ccgggcaaaa tggtgccggg ttcatattca ccttttaaga tccgttgcgc
    29821 cagcttctca gccagaacat acgaaaggtt tttctgtgca gctaactgtt gtgcgcttaa
    29881 aggcattact aatcttcctt tttcttttta ttcctcctta gtatgccacc aggaagtgtg
    29941 attacggttg caaaaacggc aaattgcttg ttttatggca cattaacggg gcttttgctg
    30001 aaaaaatgcg cggtcagaaa attattttaa atttcctctt gtcagaccgg aataactccc
    30061 tataatgcgc caccactgac acggaacaac ggcaagcacg ccgccgggtc agcggggttc
    30121 tcctgagaat tccggcagag aaagcaaaaa taaatgcttg actctgtagc gggaaagcgt
    30181 attatgcaca ccccgcgccg ctgagaaaaa gcgaagcggc actgctcttt aacaatttat
    30241 cagacaatct gtgtgggcac tcgaagatac ggattcttaa cgtcgcaaga cgaaaaatga
    30301 ataccaagtc tcaagagtga acacgtaatt cattacgaag tttaattctt tgagcatcaa
    30361 acttttaaat tgaagagttt gatcatggct cagattgaac gctggcggca ggcctaacac
    30421 atgcaagtcg aacggtaaca ggaagaagct tgcttctttg ctgacgagtg gcggacgggt
    30481 gagtaatgtc t
//
LOCUS       DABSYT020000051        29819 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.051, whole genome shotgun sequence.
ACCESSION   DABSYT020000051 DABSYT020000000
VERSION     DABSYT020000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.051"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(130..1794)
                     /gene="glnS"
                     /locus_tag="ID725_RS13655"
                     /old_locus_tag="pgaptmp_002731"
     CDS             complement(130..1794)
                     /gene="glnS"
                     /locus_tag="ID725_RS13655"
                     /old_locus_tag="pgaptmp_002731"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGV7851299.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1997..3943)
                     /gene="nagE"
                     /locus_tag="ID725_RS13660"
                     /old_locus_tag="pgaptmp_002732"
     CDS             complement(1997..3943)
                     /gene="nagE"
                     /locus_tag="ID725_RS13660"
                     /old_locus_tag="pgaptmp_002732"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGV7851300.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4276..5076
                     /gene="nagB"
                     /locus_tag="ID725_RS13665"
                     /old_locus_tag="pgaptmp_002733"
     CDS             4276..5076
                     /gene="nagB"
                     /locus_tag="ID725_RS13665"
                     /old_locus_tag="pgaptmp_002733"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGV7851301.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5136..6284
                     /gene="nagA"
                     /locus_tag="ID725_RS13670"
                     /old_locus_tag="pgaptmp_002734"
     CDS             5136..6284
                     /gene="nagA"
                     /locus_tag="ID725_RS13670"
                     /old_locus_tag="pgaptmp_002734"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGV7851302.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6293..7513
                     /gene="nagC"
                     /locus_tag="ID725_RS13675"
                     /old_locus_tag="pgaptmp_002735"
                     /gene_synonym="nagR"
     CDS             6293..7513
                     /gene="nagC"
                     /locus_tag="ID725_RS13675"
                     /old_locus_tag="pgaptmp_002735"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGV7851303.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7561..8313
                     /gene="nagD"
                     /locus_tag="ID725_RS13680"
                     /old_locus_tag="pgaptmp_002736"
     CDS             7561..8313
                     /gene="nagD"
                     /locus_tag="ID725_RS13680"
                     /old_locus_tag="pgaptmp_002736"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGV7851304.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8570..10234
                     /gene="asnB"
                     /locus_tag="ID725_RS13685"
                     /old_locus_tag="pgaptmp_002737"
     CDS             8570..10234
                     /gene="asnB"
                     /locus_tag="ID725_RS13685"
                     /old_locus_tag="pgaptmp_002737"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGV7851305.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10614..10690
                     /locus_tag="ID725_RS13690"
                     /old_locus_tag="pgaptmp_002738"
     tRNA            10614..10690
                     /locus_tag="ID725_RS13690"
                     /old_locus_tag="pgaptmp_002738"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10648..10650,aa:Met,seq:cat)
     gene            10701..10785
                     /locus_tag="ID725_RS13695"
                     /old_locus_tag="pgaptmp_002739"
     tRNA            10701..10785
                     /locus_tag="ID725_RS13695"
                     /old_locus_tag="pgaptmp_002739"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10735..10737,aa:Leu,seq:tag)
     gene            10809..10883
                     /locus_tag="ID725_RS13700"
                     /old_locus_tag="pgaptmp_002740"
     tRNA            10809..10883
                     /locus_tag="ID725_RS13700"
                     /old_locus_tag="pgaptmp_002740"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10841..10843,aa:Gln,seq:ttg)
     gene            10918..10992
                     /locus_tag="ID725_RS13705"
                     /old_locus_tag="pgaptmp_002741"
     tRNA            10918..10992
                     /locus_tag="ID725_RS13705"
                     /old_locus_tag="pgaptmp_002741"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10950..10952,aa:Gln,seq:ttg)
     gene            11008..11084
                     /locus_tag="ID725_RS13710"
                     /old_locus_tag="pgaptmp_002742"
     tRNA            11008..11084
                     /locus_tag="ID725_RS13710"
                     /old_locus_tag="pgaptmp_002742"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11042..11044,aa:Met,seq:cat)
     gene            11133..11207
                     /locus_tag="ID725_RS13715"
                     /old_locus_tag="pgaptmp_002743"
     tRNA            11133..11207
                     /locus_tag="ID725_RS13715"
                     /old_locus_tag="pgaptmp_002743"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11165..11167,aa:Gln,seq:ctg)
     gene            11245..11319
                     /locus_tag="ID725_RS13720"
                     /old_locus_tag="pgaptmp_002744"
     tRNA            11245..11319
                     /locus_tag="ID725_RS13720"
                     /old_locus_tag="pgaptmp_002744"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11277..11279,aa:Gln,seq:ctg)
     gene            complement(11473..12648)
                     /gene="ubiF"
                     /locus_tag="ID725_RS13725"
                     /old_locus_tag="pgaptmp_002745"
     CDS             complement(11473..12648)
                     /gene="ubiF"
                     /locus_tag="ID725_RS13725"
                     /old_locus_tag="pgaptmp_002745"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGV7851306.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12794..14218
                     /gene="miaB"
                     /locus_tag="ID725_RS13730"
                     /old_locus_tag="pgaptmp_002746"
     CDS             12794..14218
                     /gene="miaB"
                     /locus_tag="ID725_RS13730"
                     /old_locus_tag="pgaptmp_002746"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGV7851307.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14371..15411
                     /locus_tag="ID725_RS13735"
                     /old_locus_tag="pgaptmp_002747"
     CDS             14371..15411
                     /locus_tag="ID725_RS13735"
                     /old_locus_tag="pgaptmp_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGV7851308.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15408..15875
                     /gene="ybeY"
                     /locus_tag="ID725_RS13740"
                     /old_locus_tag="pgaptmp_002748"
     CDS             15408..15875
                     /gene="ybeY"
                     /locus_tag="ID725_RS13740"
                     /old_locus_tag="pgaptmp_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGV7851309.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15965..16843
                     /gene="corC"
                     /locus_tag="ID725_RS13745"
                     /old_locus_tag="pgaptmp_002749"
     CDS             15965..16843
                     /gene="corC"
                     /locus_tag="ID725_RS13745"
                     /old_locus_tag="pgaptmp_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGV7851310.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16868..18406
                     /gene="lnt"
                     /locus_tag="ID725_RS13750"
                     /old_locus_tag="pgaptmp_002750"
     CDS             16868..18406
                     /gene="lnt"
                     /locus_tag="ID725_RS13750"
                     /old_locus_tag="pgaptmp_002750"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGV7851311.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18803..19711
                     /gene="gltI"
                     /locus_tag="ID725_RS13755"
                     /old_locus_tag="pgaptmp_002751"
     CDS             18803..19711
                     /gene="gltI"
                     /locus_tag="ID725_RS13755"
                     /old_locus_tag="pgaptmp_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGV7851312.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19881..20621
                     /gene="gltJ"
                     /locus_tag="ID725_RS13760"
                     /old_locus_tag="pgaptmp_002752"
     CDS             19881..20621
                     /gene="gltJ"
                     /locus_tag="ID725_RS13760"
                     /old_locus_tag="pgaptmp_002752"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGV7851313.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20621..21295
                     /gene="gltK"
                     /locus_tag="ID725_RS13765"
                     /old_locus_tag="pgaptmp_002753"
     CDS             20621..21295
                     /gene="gltK"
                     /locus_tag="ID725_RS13765"
                     /old_locus_tag="pgaptmp_002753"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGV7851314.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21295..22020
                     /gene="gltL"
                     /locus_tag="ID725_RS13770"
                     /old_locus_tag="pgaptmp_002754"
     CDS             21295..22020
                     /gene="gltL"
                     /locus_tag="ID725_RS13770"
                     /old_locus_tag="pgaptmp_002754"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGV7851315.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22138..23073
                     /gene="rihA"
                     /locus_tag="ID725_RS13775"
                     /old_locus_tag="pgaptmp_002755"
     CDS             22138..23073
                     /gene="rihA"
                     /locus_tag="ID725_RS13775"
                     /old_locus_tag="pgaptmp_002755"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGV7851316.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23157..24827
                     /gene="hscC"
                     /locus_tag="ID725_RS13780"
                     /old_locus_tag="pgaptmp_002756"
     CDS             23157..24827
                     /gene="hscC"
                     /locus_tag="ID725_RS13780"
                     /old_locus_tag="pgaptmp_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGV7851317.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(24887..26338)
                     /gene="djlC"
                     /locus_tag="ID725_RS13785"
                     /old_locus_tag="pgaptmp_002757"
                     /pseudo
     CDS             complement(24887..26338)
                     /gene="djlC"
                     /locus_tag="ID725_RS13785"
                     /old_locus_tag="pgaptmp_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(26335..27042)
                     /locus_tag="ID725_RS13790"
                     /old_locus_tag="pgaptmp_002758"
     CDS             complement(26335..27042)
                     /locus_tag="ID725_RS13790"
                     /old_locus_tag="pgaptmp_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGV7851318.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            27144..27698
                     /locus_tag="ID725_RS13795"
                     /old_locus_tag="pgaptmp_002759"
     CDS             27144..27698
                     /locus_tag="ID725_RS13795"
                     /old_locus_tag="pgaptmp_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGV7851319.1"
                     /translation="MNKKLMYIFTIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(27708..29135)
                     /locus_tag="ID725_RS13800"
                     /old_locus_tag="pgaptmp_002760"
     CDS             complement(27708..29135)
                     /locus_tag="ID725_RS13800"
                     /old_locus_tag="pgaptmp_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGV7851320.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(29132..29818)
                     /locus_tag="ID725_RS13805"
                     /old_locus_tag="pgaptmp_002761"
     CDS             complement(29132..29818)
                     /locus_tag="ID725_RS13805"
                     /old_locus_tag="pgaptmp_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGV7851321.1"
                     /translation="MLFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSSWGIDDRD
                     DLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAECC
                     GEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQYFAGYTF
                     GRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQPLADYPTC
                     PETLKDMSDL"
BASE COUNT         7434 a         7672 c         7481 g         7232 t
ORIGIN      
        1 tcaatatatt gcagagatca tgtaggcctg ataagcgtag cgcatcaggc aatttagcgt
       61 ttgaaactgt ttgtctgttc cttgccggat gcgacgtaaa cgccccatcc ggcatagcga
      121 aacttaaaat tactcgccta ctttcgccca ggtatcacgc agcccaacgg tgcggttaaa
      181 taccggtttt tccgccgtag aatggcggct atcgaggcag aagtaacctt cacgctcaaa
      241 ctggaatgct ttacccgcaa ccgcatcttt cagcgacggt tcagcaaagc cctgtttgat
      301 caccagcgat tccgggttaa tcaccgacag gaaatcatcc gcagcacctg ggttcggcac
      361 gctgaacaga cggtcataca aacggatttc aaccggcagc gcatgtgccg cgctcaccca
      421 gtgaataaca cctttgactt tacgaccatc tgccggatct ttgcttaagg tatcggcgtc
      481 ataagtacag aagatggtgg tgatattacc ttcggcatct ttctcgacgc gttcagcctt
      541 aatcacataa gcattacgca gacgcacttc tttacccagc accagacgtt tgtactgctt
      601 gttagcttct tcgcggaaat cggagcgatc aatccaaatc tcaccgctaa acggcacctg
      661 acggctcccc atttccggtt tgttcggatg gttcggcatg gtaaccattt cgccttcgcc
      721 ctgatagttt tcgataacca gtttcaccgg atcgataacc gccattgcgc gcggcgcatt
      781 ttcgttgaga tcttcacgga tgcaggattc cagcgacgcc atctcaatgg tgttgtcctg
      841 cttggtcacg ccgatgcgtt tgcagaactc acgaatagaa gccgcagtgt aaccacgacg
      901 acgcagaccg gaaatggtcg gcatacgcgg gtcatcccag ccttcaacgt gcttgtcggt
      961 caccagcaag ttcagcttac gcttggacat cacagtgtat tccagattca ggcgcgagaa
     1021 ttcgtactgg cgcgggtgaa caggaatggt gatgttgtcc agtacccagt cgtacagacg
     1081 acggttatcc tggaactcaa gcgtacacag agagtgcgta ataccttcca gggcatcgct
     1141 gatgcagtgg gtgaagtcgt acatcgggta gatgcaccac ttgttgccag tctggtggtg
     1201 ttcagcaaat ttaatgcggt acagcaccgg atcgcgcatc acgataaacg gtgaagccat
     1261 gtcgattttc gcacgcaggc aggctttacc ttcttcaaaa ccgccggtac gcattttctc
     1321 gaacagcgcc aggttctctt caacgctacg gtcgcggtac gggctgtttt taccaggctg
     1381 cgtcagggtg ccgcggtatt cgcggatctg ttccggcgtc agttcatcaa cgtacgccag
     1441 gcctttattg atcagttcga tcgcataggc gtggagctga tcaaaataat cggaggagta
     1501 acggacgtta ccagaccagt gaaaacctaa ccactcaacg tcgtttttga tcgaatcgac
     1561 atactcgata tcttctttta ccgggttagt gtcgtcgaaa cgcaggttgc actggccttt
     1621 atagtcctgg gcgatcccga agttcaggca gatagatttc gcatggccaa tatgcagata
     1681 gccattcggc tccggcggga aacgggtgtg tactgtggtg tgcttaccac tggccagatc
     1741 ttcatcgatg atctgacgga taaagttagt cgggcgggct tctgcctcac tcatcgtgga
     1801 ttcctcaaag cgtaaacaac gtataacggc gtatgatctt ataagcggga caggctgaca
     1861 actgttagtt agtgaaaatc gaaaatctca ctgagattat ggggatgatt gatgaaaaaa
     1921 aaagcggcag agaagatctc caccgcttgt cattgttgga tgcgacgctc aagcgtcgca
     1981 tcaggcataa agcagattac tttttgattt catacagcgg tgtttgaccc gccacaacat
     2041 ggccctgagc tttaatgatc aagccactga aatcgtcgat attgctgcaa accaccgggc
     2101 taatcatcga acgggcgtta gcgttcaggt aatccagatc catttccaga atcggttgcc
     2161 ctgcgcttac ctgcgcgccc tcttccacca gacgtttaaa gcctttacct tccagcgcta
     2221 cggtatcgat acccatgtga acgacaatct ccgcgccttt ttcggtttcc aggcagaacg
     2281 cgtggttggt gttgaagatt ttcacgattg ttcctgcggc tggtgatacg acgattttat
     2341 ctgtcggttt caccgccaca ccgtcaccca ccgctttgct ggcgaatgct tcgtcaggaa
     2401 cctgatccag tgccacgaca tcaccggtaa tcggcgatac cagctccgcg atagataccg
     2461 cgtttggtac agcctgcggt tttgctacag gagcggcagt tgccggagtt gcttcagcag
     2521 aggcagctgc taccgggcca cgggcaacga ctttcttcat cgcatcgccg atggattctg
     2581 ctttcgcgcc aacaatcacc tgaatagtct gtttgttcag ttttactacc ccagaagccc
     2641 ccagacgttt acacatcgta tcgttaacgc gggcagagtc agccacggta aggcgcagac
     2701 gggtgataca ggcgtcaatt gctttcaggt tgtcagtgcc gccaaccgca gcaatatagt
     2761 tggtcgccag ttgattgagg ccttcttcag tgttgctgtt ggcttcttca gtaacgatct
     2821 cgtcttcttt atcttcacga cccggcgttt tcaggttgaa catgcggata accaaactga
     2881 acaccacgaa gtagatagcg aagaagacaa cacccataac cagcagcatc cagacgttct
     2941 ggctggcggc cggcaggtta tacatcaacg cgtagtcgat agcccccgca gagaaagaga
     3001 agccggcatg gatacccagc agcgttgcca caaacaggct gataccggtc agcagtgcgt
     3061 gcaggaggta cagcagcgga gcaaggaaca tgaacaggaa ttccagcggc tcagtcacac
     3121 cggtcaggaa cgcagtaaca gcaacagaaa gcagcatccc gccaaccatc ggacgacgct
     3181 ctttcggtgc tgcgaagtac atcgccagcg ccgcacccgg caggccgaac atcatgatcg
     3241 ggaagaagcc ggacatgaac atccccgcgg tgccgtcacc agcgtagaaa cggttgatgt
     3301 cgccgtggaa aaccgtaccc gccgcgttgg tgaattcacc aatctggaac caggcgatgg
     3361 tgttcagcac ctgatgcaga ccggttggga tcaacagacg gttgatgaaa ccaaagatac
     3421 cggaacccag cgcgcccgca gaaacgatcc actcgccgcc tgcatggata gcgtgctgta
     3481 ccggcggcca gacgtaacca aaaatggccg ccagcaccag acagaagaag ccggtggcga
     3541 tcggcacaaa gcgtttgccg ccgaagaagc tcaggaagtc cggcagttta atatcggacc
     3601 aacggttata ggctgcgcca ccaaccagac cggtaatgat acccgccagt acacccatgt
     3661 taatttctgg gttgatggtc accatcgctt tggttaacac aaagtaacct accgcccccg
     3721 ccagcgccgc cgcacctgcg ctgtctttcg accagctgga tgccacaccg atggcgaaga
     3781 ttaatgcgag gttatcaaaa atcgcaccgc ccgcctgggc aataaacgca acgttaagta
     3841 aatctggctg accgaatcgc agcaacagtg ccgccaccgg cagcaccgcg atagggagct
     3901 gtaacgccct accgagtcgc tggaaaaaac ctaaaatatt catcttattc cccctacgag
     3961 aaccctattt ggctcgttta aagccgtatt tttattgtgc tgcaaattgt actgccgatg
     4021 ttctgtaatc agattgttag atcatctgcc acagagtgtg tgaaaattta attcgtatcg
     4081 caaattaaac gagtgtcttt tgtgagtttt gtcaccaaat atcgttatca tcactccctt
     4141 ttactggcta aaccagaaaa cttattttat cattcaaaaa atcaggtcgg attgacgcct
     4201 gtctgcgcaa atccaggtta cgcttaaaga tgcctaatcc gccaacggct tacattttac
     4261 ttattgaggt gaataatgag actgatcccc ctgactaccg ctgaacaggt cggcaaatgg
     4321 gctgctcgcc atatcgtcaa tcgtatcaat gcgttcaaac cgactgctga tcgtccgttt
     4381 gttctgggcc tgccaactgg cggcacgcca atgaccacct ataaagcgtt agtcgaaatg
     4441 cataaagcag gccaggtcag ctttaagcac gttgtcacct tcaacatgga cgaatatgtc
     4501 ggtctgccga aagagcatcc ggaaagctac tacagcttta tgcaccgtaa tttcttcgat
     4561 cacgttgata ttccagcaga aaacatcaac cttctcaacg gcaacgcccc ggatatcgac
     4621 gccgagtgcc gccagtatga agaaaaaatc cgttcttacg gaaaaattca tctgtttatg
     4681 ggcggtgtag gtaacgacgg tcatattgca tttaacgaac cggcgtcttc tctggcttct
     4741 cgtactcgta tcaaaaccct gactcatgac actcgcgtcg cgaactctcg tttctttgat
     4801 aacgatgtca atcaggtgcc aaaatatgcc ctgactgtcg gtgttggtac actgctggat
     4861 gccgaagaag tgatgattct ggtgctgggt agccagaaag cactggcgct gcaggccgca
     4921 gttgaaggtt gcgtgaacca tatgtggacc atcagctgtc tgcaactgca tccgaaagcc
     4981 atcatggtgt gcgatgagcc ttccaccatg gagctgaaag ttaagacttt aagatatttc
     5041 aatgaattag aagcagaaaa tatcaaaggt ctgtaattgt tatccctgcc ctggctcctt
     5101 gctcagggca atatttttta aaatcggggg tcagaatgta tgcattaacc cagggccgga
     5161 tctttaccgg ccacgaattt cttgatgacc acgcggttgt tatcgctgat ggcctgatta
     5221 aaagcgtctg tccggtagcg gaactgccgc cagagatcga acaacgttca ctgaacgggg
     5281 ccattctctc ccccggtttt atcgatgtgc agttaaacgg ctgcggcggc gtacagttta
     5341 acgacaccgc tgaagcggtc agcgtggaaa cgctggaaat catgcagaaa gccaatgaga
     5401 aatcaggctg tactaactat ctgccgacgc ttatcaccac cagcgatgag ctgatgaaac
     5461 agggcgtgcg cgttatgcgc gagtacctgg caaaacatcc gaatcaggcg ttaggtctgc
     5521 atctggaagg cccgtggctg aatctggtga aaaaaggtac ccataatccg aattttgtgc
     5581 gtaagcctga tgccgcgctg gtcgatttcc tgtgtgaaaa cgccgacgtc attaccaaag
     5641 tgaccctggc accggaaatg gttcctgcgg aagtcatcag caaactggca aatgccggga
     5701 ttgtggtttc tgccggtcac tccaacgcga cgttgaaaga agcgaaagcc ggtttccgcg
     5761 cggggattac ctttgccacc catctgtaca acgcgatgcc gtatattacc ggtcgtgaac
     5821 cgggcctggc gggcgcgatc ctcgatgaag ctgacattta ttgcggtatt atcgctgatg
     5881 gcctgcatgt tgattacgcc aacattcgta atgctaaacg ccttaaaggc gacaaactgt
     5941 gtctggttac cgacgccacc gcgccagcag gtgccaacat tgaacagttc atttttgcgg
     6001 gtaaaacaat atactaccgt aacggacttt gtgtggatga gaacggtacg ttaagcggtt
     6061 catccttaac catgattgaa ggcgtgcgta atctggtcga acattgcggt atcgcactgg
     6121 atgaagtgct gcgtatggcg acgctctatc cggcgcgtgc gattggcgtt gagaaacgtc
     6181 tcggcacact cgccgcaggt aaagtagcca acctgaccgc attcacacct gattttaaaa
     6241 tcaccaagac catcgttaac ggtaacgagg tcgtaactca ataagagaaa gtatgacacc
     6301 aggcggacaa gctcagatag gtaatgttga tctcgtaaaa cagcttaaca gcgcggcggt
     6361 ttatcgcctg attgaccagt acgggccaat ctcgcggatt cagattgccg agcaaagcca
     6421 gcttgccccc gccagcgtaa ccaaaattac gcgtcagctt atcgaacgcg ggctgatcaa
     6481 agaagttgat cagcaggcct ccaccggggg ccgccgcgct atctctatcg tcaccgaaac
     6541 ccgcaatttc cacgcaatcg gcgtacggct tggtcgtcac gacgccacca tcaccctgtt
     6601 tgatctcagc agcaaagtgc tggcagaaga acattacccg ctgccggaac gtacccagca
     6661 gacgctggaa catgccctgc tgaatgccat tgctcagttt attgatagct accagcgcaa
     6721 actgcgcgag ctgatcgcca tttccgttat cctgccagga cttgttgacc cggacagcgg
     6781 caaaattcat tacatgccgc atattcaggt agaaaactgg gggctggtag aagcactgga
     6841 agagcgtttt aaagtgacct gtttcgttgg tcacgatatc cgtagtctgg cgctggcgga
     6901 gcactacttc ggtgcaagtc aggattgcga agactccatt ctggtgcgtg tccatcgcgg
     6961 aaccggggcc gggattatct ctaacgggcg catttttatt ggccgcaacg gcaacgtcgg
     7021 tgaaattggc catattcagg tcgaaccgct gggtgaacgc tgccactgcg gcaactttgg
     7081 ctgcctggaa actatcgctg ccaacgccgc tattgaacaa cgggtgttga atctgttaaa
     7141 gcagggctac cagagccgcg tgccgctgga cgactgcacc atcaaaacta tctgcaaagc
     7201 cgcgaacaaa ggcgatagcc tggcctcgga agtgattgag tatgtcggtc gtcatctggg
     7261 caaaaccatc gccattgcta tcaacttatt taatccgcaa aaaattgtta ttgccggtga
     7321 aatcaccgaa gccgataaag tgctgctccc tgctattgaa agctgcatta atactcaggc
     7381 gctgaaggcg tttcgtacta atctgccggt ggtacgttct gagctagacc accgctcggc
     7441 aatcggtgct tttgcgctgg taaaacgcgc catgctcaac ggtattttgc tccagcattt
     7501 gctggaaaat taatgtgctt ttatagtggc gcttattgtt gtcaatattc tgggtagtcc
     7561 atgaccatta aaaatgtaat ttgcgatatc gacggcgtgc tgatgcacga taacgtcgcc
     7621 gtaccgggtg cagcggaatt tttgcacggg attatggata aaggcctgcc gctggtgttg
     7681 ctgaccaact atccttcgca gaccgggcaa gatctggcga accgctttgc cactgccggt
     7741 gtcgatgtac ctgacagcgt gttttatacc tctgcgatgg cgaccgccga tttcctgcgt
     7801 cgccaggaag gcaagaaagc gtatgttgtg ggcgaaggcg cactgattca tgaactgtac
     7861 aaagccggtt tcactattac cgatgtgaac cctgattttg tgattgttgg cgaaacgcgt
     7921 tcctacaact gggacatgat gcataaagcg gcctatttcg tcgctaacgg tgcacgcttt
     7981 atcgccacca acccggacac ccacgggcgc ggtttttatc cggcctgtgg cgcgttgtgt
     8041 gcagggattg agaaaatctc cgggcgcaaa ccgttctatg ttggtaagcc gagtccgtgg
     8101 attatccgcg cggcattaaa caaaatgcag gcacattcgg aagaaacggt gattgtcggc
     8161 gataacctgc gtaccgatat tctggccggc ttccaggcag gtctggagac gattctggtg
     8221 ctttctggtg tttcgtcgct cgacgatatc gacagtatgc ctttccgccc cagctggatt
     8281 tacccgtcag tcgctgaaat cgacgttatc tgaatagcaa ccacgtctca ggacgtggtt
     8341 tcttattttt acccatcatt aaaatcatcc cattcaataa aacccgccaa aaattatcga
     8401 tccatcaata aatacatcac cgcaccctcc cctttttcac aaatcagcaa tcaccattac
     8461 gttttttatt ttttcaccgc caaatcgctt gcgcacagtg ctgctttgcg gcattttttt
     8521 aaacaagcaa acacaacaag caacaaatac caggttaacg gagaaggtta tgtgttcaat
     8581 ttttggcgta ttcgatatca aaacagacgc agttgagctg cgtaagaaag cactcgagct
     8641 gtcacgcctg atgcgtcatc gtggcccgga ctggtccggt atttatgcca gcgataaagc
     8701 cattctcgct cacgaacgtc tgtcaattgt tgacgttaac gcgggggcgc aacctctcta
     8761 caaccaacaa aaaacccacg tactggcggt aaacggtgaa atctacaatc accaggcatt
     8821 gcgcgctgaa tatggcgatc gttatcagtt ccagacaggg tctgactgtg aagtgatcct
     8881 cgcgctgtat caggaaaaag ggccggaatt tctcgacgac ttgcagggca tgtttgcctt
     8941 tgccctgtac gacagcgaaa aagatgccta cctgattggt cgcgaccatc tggggatcat
     9001 cccactgtat atgggctatg acgaacacgg tcagctgtat gtggcctcag aaatgaaagc
     9061 cctggtgcca gtttgccgca cgattaaaga gttcccggcg gggagctatt tgtggagtca
     9121 ggacggcgaa attcgttctt attatcatcg cgactggttc gactttgatg cagtgaaaga
     9181 taacgtgacc gacaaaaacg agctgcgtca ggctctggaa gattccgtta aaagccatct
     9241 gatgtctgat gtgccttacg gtgtgctgct ttctggtggt ctggattcct caattatttc
     9301 cgctatcacc aagaaatacg cagcccgtcg cgtggaagat caggaacgct ctgaagcctg
     9361 gtggccgcag ttacactcct ttgctgtagg tctgccgggt tcaccggatc tgaaagcagc
     9421 ccaggaagtg gcaaaccatc tgggcactgt gcatcatgaa attcacttca ctgtacagga
     9481 aggtctggac gccatccgcg acgtgattta ccacatcgaa acctatgatg tgaccactat
     9541 tcgcgcttca acaccgatgt atttaatgtc gcgtaagatc aaagcgatgg gcattaaaat
     9601 ggtgctgtcc ggtgaaggtt ctgatgaagt gttcggcggt tatctttact tccacaaagc
     9661 accgaatgcc aaagaactgc atgaagagac ggtgcgtaaa ctgctggccc tgcatatgta
     9721 tgactgcgcg cgtgccaaca aagcgatgtc agcctggggc gtggaagcac gcgttccgtt
     9781 cctcgacaag aaattcctcg acgtggcgat gcgcattaac ccgcaggata aaatgtgcgg
     9841 taacggcaag atggaaaaac acatcctgcg tgaatgtttt gagtcatacc tgcccgcaag
     9901 cgtggcctgg cggcagaaag agcagttctc cgatggcgtc ggttacagtt ggatcgacac
     9961 cctgaaagaa gtggcggcgc agcaggtttc tgatcaacag ctggaaactg cccgcttccg
    10021 cttcccgtac aacacaccga cctcaaaaga agcgtatctg taccgggaga tctttgaaga
    10081 actattcccg cttccgagcg ccgctgagtg cgtgccgggc ggtccttccg tcgcgtgttc
    10141 ttccgctaaa gcgatcgaat gggatgaagc gttcaagaaa atggacgatc cgtctggtcg
    10201 cgcggttggt gttcaccagt cggcgtataa gtaagaaatg cgaaacagcc ccggtgaaat
    10261 ccggggctgt ttcagttatt gaggtgcttg ttgattctga tatgcagtaa ggaaatgttc
    10321 cgggtttgcc acatcactgt ttgacaactc atcactgtag gccagtattt catagattaa
    10381 tttcccacca ctattgttaa attccgaatt tcatcctgta tgacaaaaga atggctgtag
    10441 ggtgggaaaa aacacaaagt aaacaataat tgacgaatat agcgccacgc tgttcgcaac
    10501 ctaaccaaac agtcactttc gagcaatttt ccttgaaaaa gaggttgacg ctgcaaggct
    10561 ctatacgcat aatgcgcccc gcaacgccga taaggtatcg cgaaaaaaaa gatggctacg
    10621 tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc
    10681 gtagccacca tctttttttt gcgggagtgg cgaaattggt agacgcacca gatttaggtt
    10741 ctggcgccgc aaggtgtgcg agttcaagtc tcgcctcccg caccattcac cagaaagcgt
    10801 tgtacggatg gggtatcgcc aagcggtaag gcaccggttt ttgataccgg cattccctgg
    10861 ttcgaatcca ggtaccccag ccatcttctt tgagtaagcg gttcaccgcc cggttattgg
    10921 ggtatcgcca agcggtaagg caccggtttt tgataccggc attccctggt tcgaatccag
    10981 gtaccccagc catcgaagaa acaatctggc tacgtagctc agttggttag agcacatcac
    11041 tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc accaaattct gaatgtgtcg
    11101 aatacgttcg gcaaattcaa aaaccaattt gttggggtat cgccaagcgg taaggcaccg
    11161 gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccaatt tattcaagac
    11221 gcttaccttg taagtgcacc cagttggggt atcgccaagc ggtaaggcac cggattctga
    11281 ttccggcatt ccgaggttcg aatcctcgta ccccagccac attaaaaaag ctcgcttcgg
    11341 cgagcttttt gcttttctgc gttcactcaa tgtcgaatgc gatgttgaca cgtcttatcc
    11401 ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct acggttggca cgcatccggc
    11461 aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc tgacgtttca acacgccagc
    11521 acgctccgcc gccatcaaac caagattacg cacaaaacgc agcggcggca gattattact
    11581 gaatccggca taaaacagat ccataccgct ctgcataatg aagttatccg ccatgcgccg
    11641 catctggtaa cgcttgagga caggataact ggcccacgct tcgccgtagc tgcgggcatt
    11701 caccagaaca tcaatcaggg catcgacatc acgataacca agattcaccc cctgccccgc
    11761 cagcggatgg atggtgtgcg cggcatcacc caccagcgca agccccggct gcacatactg
    11821 caacgcatgg cggcgtgtca gcggaaacgc accagcggca agcggtgtaa cgtaacccag
    11881 acgcgacggg aaatgcttcg cgatttccac ctgtaactgc gccatattca tattctgcaa
    11941 ctggcgaatg cgcgccggag agtcgtacca caccagcgat gcccagttat caaacagcgg
    12001 cagaaacgcg cgcggtccat ccggggtaaa ttgctgccag gtgctatcgc cgggatcgtt
    12061 ctcgcactgg acgctaatca acatgcacga ctgcgcatac tgccatgcat gaacgccaat
    12121 tcccgccatc tgccgcacct gcgaatttgc gccgtcggca ccaatcacca gcttcgcgcg
    12181 aatcacttca ccgcctttca gctccagctc ctgaagatca ttatctcgat gtaatgcaat
    12241 cagcgaggtt ggcacacgta acgttacttt cggatgcgct tccagcgcct gccacaatgc
    12301 ctgttgcagg acagtgtttt ctaccatata gccaagcaac ggcaacttta attcggctgc
    12361 gtcaaatacc acatgcgccg tttcccactc ccacgtttcc agtctgcggt aaggatggca
    12421 acgcatagcc tgtactgcat cccagacccc taacccttta agcaatgata ccgaagccgc
    12481 gctgatcgcc gagatccgca cgtccggttg gctatcagcg acaaacggtg caggttctgc
    12541 gtgctcgatc accgttaccg taaatccgtg ctgtgccagc cccagcgcca gtgcgccgcc
    12601 gaccattcct ccgccgacaa tggcaatttc cgttggttga tttgtcatgg tcaatcatcc
    12661 tgttagcaat atgctttaag tttacaggat ttttaccccc aagggctgat aacgctcata
    12721 ctggtcacaa cggcagcaaa gcattacact atgcgccctg cattcctggc tactatttcg
    12781 caagagcaag tcgatgacca aaaaactcca tattaaaacc tggggctgtc agatgaacga
    12841 gtacgattca tcgaagatgg ccgatctgct ggatgccacc cacggctatc aactgaccga
    12901 cgtggcggaa gaagcggatg tgctgctgct gaacacctgc tcaatccgcg agaaggctca
    12961 ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta aaagagaaga atccagacct
    13021 gattatcggc gtcggtggct gcgtggcatc gcaagaaggc gagcacattc gccagcgcgc
    13081 ccactatgtc gatattattt ttgggccgca aacgctgcac cgtctgccgg agatgatcaa
    13141 ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc ttcccggaaa tcgagaagtt
    13201 tgaccgtcta ccggaaccgc gcgccgaagg gccgaccgcg tttgtctcca tcatggaagg
    13261 ctgcaataaa tattgcacct actgcgtggt gccttacacc cgtggtgaag aggtaagccg
    13321 tccgtctgac gatattctgt ttgagattgc ccagcttgcg gctcagggcg tgcgtgaagt
    13381 caacctgctc ggtcagaacg tgaacgcctg gcgtggtgaa aactacgacg gcaccaccgg
    13441 atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac gggatcgatc gtattcgctt
    13501 taccaccagc catccgatcg aattcaccga cgatatcatc gaagtgtatc gcgacacgcc
    13561 ggagctggtg agcttcctgc atctgccggt acagagcggt tccgatcgca ttctgaacct
    13621 gatgggacgt acccatacgg cgctggagta caaagcgatc atccgtaaac tgcgtgcggc
    13681 gcgtccagat attcagatca gttctgactt catcgtcggc ttccctggcg aaaccaccga
    13741 agacttcgag aaaacgatga agctgattgc cgacgtcaat ttcgacatga gctacagctt
    13801 tatcttctct gcacgtccgg gtacaccagc cgccgatatg gttgatgatg ttccggaaga
    13861 agagaagaag cagcgtctgt atattctgca agaacgtatc aatcagcaag cgatggcgtg
    13921 gagccgccgg atgctcggca ccacccagcg tattctggta gaaggtacat cgcgtaagag
    13981 catcatggag ctttccgggc gtacggaaaa taaccgcgtg gtcaacttcg aaggcacgcc
    14041 ggatatgatc ggtaaattcg tcgatgtaga aattaccgac gtctacccga actctctgcg
    14101 cggtaaagtg gtgcgtactg aagatgaaat gggtctgcgc gtggcagaaa caccagaatc
    14161 ggtgattgcc cgtactcgca aagaaaacga ccttggcgtg ggttattatc agccgtaatt
    14221 ctcaggcccg ccgttccggt gggccttgtt ttttctctct ttatccccat ctttatcgca
    14281 atgcttgcgc ctttttcctg tggcgtgaat aatttcctta taggccggtg aatgttcagc
    14341 ttccggcggc atacagataa gaggaacggt ttgaacatag acactcgcga aatcaccctg
    14401 gagccagcag acaacgcgcg tctgttgagc ctgtgcggcc cgtttgatga caacatcaag
    14461 cagctcgaac gccgtctcgg catcgagatc aatcgccgcg ataaccactt taaactgacc
    14521 ggccgtccga tttgcgtcac cgctgcggca gacattctgc gtagcctgta tgtcgatact
    14581 gccccgatgc gcggtcagat tcaggatatc gaaccggaac agatccacct tgcgattaaa
    14641 gaagcacggg tactggagca aagcgcggag agcgtgccgg agtacggcaa agcggtcaat
    14701 atcaaaacca aacgcggcgt aattaagccg cgcacgccaa accaggcgca gtacatcgcc
    14761 aatattctcg accatgacat taccttcggc gttggcccgg cgggtacggg taaaacctac
    14821 ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg aaattcgccg tattctgctg
    14881 actcgtccgg cagtagaagc cggtgagaaa ctgggcttcc tgcctggcga tttaagccag
    14941 aaagtagacc cgtatctgcg cccgctgtac gacgcgctgt ttgaaatgct gggttttgag
    15001 aaagtcgaga aactgattga gcgcaacgtt attgaagtcg caccgctggc ctatatgcgt
    15061 ggtcgtacgc tgaacgacgc atttatcatt ctcgatgaga gccagaacac taccatcgaa
    15121 cagatgaaga tgttcctgac ccgtatcggt tttaactcaa aagcggttat caccggcgac
    15181 gtcacacaga tcgacctgcc gcgtaatact aaatcaggct tacgtcatgc catcgaagtg
    15241 ctggccgatg tcgaagagat cagctttaac ttcttccaca gcgaagacgt ggttcgtcac
    15301 ccggtggtgg cgcgtatcgt taacgcctat gaagcctggg aagaagccga acaaaaacgt
    15361 aaagcggcgc tggcggcaga acgcaagcgc gaagaacagg aacaaaaatg agtcaggtga
    15421 tcctcgattt acaactggca tgtgaagata actccggatt gccggaagag agccagtttc
    15481 aaacatggct gaatgcggtg atcccgcagt ttcaggaaga gtcggaagtg acgattcgcg
    15541 tggtcgatac cgccgaaagc cacagtctca atctgaccta tcgcggtaag gataagccga
    15601 ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat ggagatgtcg ctactgggcg
    15661 atctggttat ctgccgtcag atggttgaga aggaagctca ggagcaaggc aaaccactgg
    15721 aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca tttgttaggt tacgatcaca
    15781 tcgaagatga cgaagcagaa gaaatggaag ccctcgaaac agagattatg cttgctctgg
    15841 gctatgagga tccgtacatt gccgagaaag aataatttgc cagccatttg actggcagac
    15901 gtccccgccg ttggtgatta acgacggcgt tttgattaac taacatgaga acccatacga
    15961 cgccatgagc gacgacaatt cacacagtag tgacacgata agcaacaaga agggattttt
    16021 ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa aaccgtgacg aactgctggc
    16081 gctgatccgt gattccgggc agaacgacct tatcgacgaa gatacgcgcg atatgctcga
    16141 aggggtgatg gacatcgcag accaacgcgt ccgcgacatc atgatccccc gctcccagat
    16201 gattaccctg aaacgcaacc agacgctgga cgaatgcctt gatgtcatca tcgagtccgc
    16261 ccactcacgt ttcccggtga ttagcgaaga caaagatcac attgaaggga ttctgatggc
    16321 gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc ttcagcatgg acaaagtgtt
    16381 acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac cggatgctga aagagtttcg
    16441 ctctcagcgt taccacatgg cgatcgttat tgacgaattc ggtggggttt ccggtctggt
    16501 aaccattgaa gacatcctgg aactgattgt tggtgagatt gaagacgaat atgacgaaga
    16561 agatgatatc gacttccgtc agctgagtcg tcatacctgg accgtgcgcg cactggcttc
    16621 cattgaagac ttcaacgaag cgttcggcac ccactttagc gatgaagaag tcgacactat
    16681 cggtggtctg gtgatgcagg catttgggca tcttccggca cgtggcgaaa ccatcgacat
    16741 cgacggttac cagttcaaag tggcgatggc cgacagtcgg cgtattattc aggttcatgt
    16801 caaaatcccg gatgactcac cccagccgaa gctggatgaa taaaaccgaa actggataga
    16861 taactacatg gcttttgcct cattaattga acgccagcgc attcgcctgc tgctggcgtt
    16921 attattcggt gcctgcggaa cgctggcctt ctctccttac gacgtctggc ctgcggcgat
    16981 tatttcgctg atggggcttc aggcgttgac ctttaaccgc cgtccactcc agtctgccgc
    17041 tattggcttt tgctggggat ttggcctctt tggcagcggt attaactggg tctatgtcag
    17101 catcgcgacc tttggcggaa tgcctggccc ggttaacatc ttcctggtgg tactgctggc
    17161 ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg ctgtcgcgcc tgtggccgaa
    17221 aaccacctgg ctgcgcgtgg cgattgccgc ccctgctctc tggcaagtga ccgagtttct
    17281 gcgcggttgg gtactgacag gcttcccgtg gttacagttc ggctatagcc agattgatgg
    17341 cccgttaaaa gggctggcac cgctaatggg cgtggaagcc attaacttcc tgcttatgat
    17401 ggttagcggc ctgctggcac tggcgttagt caaacgcaac tggcgtccgc tggtggtggc
    17461 cgtcgtgctg tttgcccttc ccttcccgct gcgttacatc cggtggttta ccccacaacc
    17521 ggagaaaacc attcaggttt cgatggttca gggcgatatt ccgcaatcgc tgaaatggga
    17581 cgaaggccag cttcttaata cgctgaagat ttactacaac gcaacggcac cgctgatggg
    17641 caaatcatcg ttgattatct ggccggagtc ggcgataacc gatctggaaa ttaatcagca
    17701 accgttcctc aaagcactgg acggtgagtt gcgtgataaa ggtagctcgc tggtgaccgg
    17761 gattgttgac gcgcgtctta ataagcagaa ccgttacgat acctacaaca ccatcatcac
    17821 actgggtaaa ggtgcaccgt acagctacga atcagccgat cgctataaca aaaaccatct
    17881 ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg cgtccgttag caccgttctt
    17941 tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc cagccgccgc tgtcggcaaa
    18001 tggtattgag cttactgcgg ctatttgcta cgagatcatt ctcggcgagc aagtgcgcga
    18061 taacttccgc ccggataccg actatctgct gactatctcc aacgatgcgt ggtttggtaa
    18121 gtctattggt ccatggcagc acttccagat ggcgcgaatg cgtgcgctgg agctggcgcg
    18181 cccactgttg cgcagcacca acaacggcat tacggcggtg attggcccgc agggtgagat
    18241 tcaggcgatg atcccgcagt tcacccgcga ggtgttaacc actaacgtga cgccgaccac
    18301 cggactcaca ccatacgcac gtaccggcaa ctggccgctg tgggtgctga cggcactgtt
    18361 tggttttgct gctgtgttga tgagtctgcg tcagcgacgt aaataatccc ttcttatcgt
    18421 gccggaatac atcccatatt tcggcacgct caccggatgc ggcatcagtg cattatccgg
    18481 ataacaatat tctcctatcc aactttggca catctattgc tttgttatac aaggcaaagc
    18541 tttaaccagc atcagtacaa cccggttgca ccacagcaat ccaccggtag caccaaaaga
    18601 gtgcatttta agaattttgc ttcggaatgg tgcggcgcac ctcccaaata taaacaataa
    18661 aacagcaata tctttacatt tagccaaact aaatgttacc taacaggcac aacactacac
    18721 aataaagttg cagatgataa caacacaaac actcacaacg ggtatccatg cgttcttaac
    18781 gcagaagata aaggagttgg atatgcaatt acgtaaacct gccacagcaa tcctggccct
    18841 ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc ccggcagcgg gcagtacgct
    18901 ggacaaaatc gccaaaaacg gcgtgattgt cgtcggtcac cgtgaatctt cagtaccttt
    18961 ctcttattac gacaatcagc aaaaagtggt gggttattcg caggattact ccaacgccat
    19021 tgttgaagca gtgaaaaaga aactcaacaa accggacttg caggtgaaac tgattccgat
    19081 tacctcacaa aaccgtattc cgctgctgca aaacggcact tttgattttg aatgtggttc
    19141 taccaccaac aacgtcgaac gccaaaaaca ggcggctttc tctgacacta ttttcgtggt
    19201 cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa gattttgccg acctgaaagg
    19261 caaagccgta gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca aactgaatga
    19321 agagcaaaaa atgaatatgc gcatcatcag cgccaaagat cacggtgact ctttccgtac
    19381 cctggaaagc ggtcgtgccg ttgcctttat gatggatgat gctctgctgg ccggtgaacg
    19441 tgcgaaagcg aagaaaccag acaactggga tatcgtcggc aagccgcagt ctcaggaagc
    19501 ctatggttgc atgttgcgta aagacgatcc gcagttcaaa aagctgatgg atgacactat
    19561 cgctcaggtg cagacctccg gtgaagcgga aaaatggttt gataaatggt tcaaaaatcc
    19621 cattccgccg aaaaacctga acatgaattt cgaactgtca gacgaaatga aagcactgtt
    19681 caaagaaccg aatgacaaag cactgaacta attacaagaa ccaggggcgg aaattctagc
    19741 cctctcgatt gttacgtagc acggacagac tatacgcctg atggtcgttc cccatcgggc
    19801 ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta gcagttaacg ctgctaccct
    19861 cttttttctg gagtagattt atgtctatag actggaactg gggtattttt ttacaacaag
    19921 ccccgttcgg caacaccacc tatcttggtt ggatctggag tggttttcag gtgacgatcg
    19981 ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg ttcatttttc ggtattttac
    20041 gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt gtatgtcgaa ctgttccgca
    20101 acgtgccgtt gattgtgcaa ttctttacct ggtatctggt gatcccggag ctgctgccgg
    20161 agaaaatcgg catgtggttt aaggccgagc tggatcccaa tattcagttt ttcctttcat
    20221 ccatgctctg cctggggctg tttaccgccg cccgtgtttg cgaacaggta cgcgccgcga
    20281 ttcagtcgct gccgcgtggg cagaagaatg ccgcgctggc tatgggtctg acgctgccgc
    20341 aggcttaccg ctacgtactg ctgcctaatg cttatcgcgt tatcgtcccg ccgatgacct
    20401 cagagatgat gaacctggtg aaaaactccg ccatcgcctc gaccatcggt ctggtagata
    20461 tggcagcgca ggcgagtaaa ttgctcgatt actcggctca cgcctgggag tcgtttaccg
    20521 ccattacgct agcgtatgtg tttattaacg cttttatcat gctggtgatg acgctggttg
    20581 aacgtaaagt tcgcctgcct ggcaacatgg ggggcaaata atgtacgagt ttgactggag
    20641 ttccattgtc ccttccctgc catatctgct cgacgggctg gtgatcacgc tgaaaatcac
    20701 cgtcacggca gtcgtgattg gtattttgtg gggcaccatg ctggcggtga tgcgcttgtc
    20761 cagctttgcg cccgtagcgt ggtttgccaa agcctacgtt aacgtattcc gctcgattcc
    20821 tttagttatg gttttgctgt ggttttacct gatcgtgccg ggttttctgc aaaacgtgct
    20881 gggattatca ccaaaaaatg atatccgttt aatttcggcg atggtggcgt tttcgatgtt
    20941 tgaagcggcc tactattcag agattatccg tgccggtatt caaagtattt ctcgtggtca
    21001 gtcgagcgcc gcgctggcgt tgggaatgac tcactggcag tcgatgaaac tgattattct
    21061 gccgcaggcg ttccgcgcga tggtgccgct gctgctcact cagggcatcg tactgttcca
    21121 ggatacctcg ctggtgtatg tgttatctct ggccgatttc ttccgtaccg cctcaaccat
    21181 tggtgagcgt gatggcacgc aggttgagat gatcctgttt gccggatttg tttatttcgt
    21241 tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa agaaggacag cataatgatt
    21301 accctgaaaa atgtttcaaa atggtatggt cactttcagg tgctgaccga ctgctcaacc
    21361 gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt ctggttccgg caaatcaacg
    21421 ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag gtgaaatcac cgtcgatggt
    21481 atcgtggtta acgacaagaa aaccgatctg gcaaagctgc gttctcgcgt cgggatggta
    21541 ttccaacatt tcgagctgtt ccctcatctg tcgattatcg aaaacctgac cctggcgcag
    21601 gtgaaagtgc ttaaacgcga taaagcaccg gctcgtgaaa aagcattgaa attgctggag
    21661 cgcgtgggac tttccgcaca tgccaataaa tttccggcgc agctttccgg cggccagcag
    21721 cagcgtgtgg caattgctcg cgcattgtgt atggatccta ttgcgatgct gttcgacgaa
    21781 cccacatcgg cactagaccc ggagatgatc aacgaagtac tggacgtgat ggtggaactg
    21841 gcgaacgagg ggatgaccat gatggtggtg acccacgaaa tgggctttgc ccgtaaagtg
    21901 gcgaatcggg tgatctttat ggatgagggt aaaattgttg aagactcgcc gaaagatgcg
    21961 ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc tcgcgaaaat cctgcattaa
    22021 tcactctggc gcgcataccc aagtgcgcgc catctcacgc tttatcgtct cgcccctcgt
    22081 atctgttaca tcctggcgtt acccttgtcg caaagaagca cacaacaagg agcaacaatg
    22141 gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg acgctatcgc aatagttctc
    22201 gccctcgcct caccagagct tgatgtcaaa gcaattacgt cttccgccgg aaaccagaca
    22261 ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct tgcttaatcg caccgatatt
    22321 ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt tgattatcgc ggacaatgtg
    22381 cacggcgaaa gcggtctcga cggcccggca ttaccggaac cgacgttcgc accgcaaaac
    22441 tgtacggcgg tagagctgat ggcgaaaacg ctgcgtgaaa gtgcggaacc tgtcacgatt
    22501 gtgtctaccg gaccgcaaac taacgttgcc ttgctgctca atagccatcc ggaactgcat
    22561 agcaaaattg cccgtatcgt gattatgggt ggcgctatgg gactgggtaa ctggacgcct
    22621 gcggctgaat ttaatattta cgttgacccg gaagcggcag aaattgtctt ccagtcaggg
    22681 atcccggtgg tgatggccgg tctggatgtt actcacaaag cacaaatcca cgttgaagac
    22741 actgagcgtt tccgcgcgat tggcaaccct gtttcaacca ttgttgccga actgctggat
    22801 ttcttcctcg aatatcataa agacgaaaaa tggggctttg tcggcgcacc gctacatgac
    22861 ccatgcacca tcgcctggct gttaaaaccg gagttgttta ccactgttga gcgctgggtt
    22921 ggcgtggaaa cacaggggaa atatacccag ggtatgacgg ttgttgatta ttattatctg
    22981 actggcaata aaccgaatgc cactgtaatg gtcgatgttg atcgtcaggg ctttgttgat
    23041 ttactggctg atcgtctgaa attttacgct taacacacgt cattgattaa gatcaccacg
    23101 cgttacctgg gtggtcttaa tctgtttatc tctttttctt tcaagatata aatgcaatgg
    23161 ataatgcaga actcgccatt ggtatcgatc tcggtactac caatagttta attgccgtct
    23221 ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg tgaatattta acaccatcca
    23281 taattagcat ggatgaaaat aatcatattt tagtcggaaa accggctgta tcacggcgta
    23341 cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc aatgggcagt aataccaact
    23401 ggcggttagg cagcgacaca tttaacgcgc cagaactgtc ctctttggta ttacgctcat
    23461 taaaagaaga tgccgaagaa tttctgcaac gtccgattaa agatgtggtg atctctgttc
    23521 cggcttattt cagcgatgaa caacgcaagc atacccgttt agcagcggag ttagccggtt
    23581 taaatgcggt acgcttaatt aatgaaccca cagcagctgc gatggcgtat ggcctgcata
    23641 cccaacaaaa tacccgttcg ctggtgtttg atctcggtgg cggcacgttt gacgtcacgg
    23701 tgcttgagta cgccacgccg gtgattgaag ttcacgcctc cgctggcgac aacttcctcg
    23761 gtggcgaaga ttttacccat atgctggtcg atgaggtttt aaaacgcgcg gatgtcgcca
    23821 ggaccacgct taacgagagt gaactggcag ccttgtatgc ctgtgtggaa gcggcaaaat
    23881 gtagcaatca atcgccaata cacattcgct ggcagtacca ggatgaaacg cgggaatgcg
    23941 aattttacga gaacgaactg gaagatttgt ggttgccgct gctcaatcgc ttgcgagtgc
    24001 cgattgaaca ggcgttgcgc gatgcgcgtc tgaagccgag tcaaatcgac agtctggtgc
    24061 tggttggcgg cgcgtcacaa atgccgctgg tgcagcgaat cgccgtgcgt ctgtttggca
    24121 aattaccgta tcaaagttac gatccgagca ccattgtcgc gctgggcgca gcaatccagg
    24181 cagcctgccg cttacgcagt gaagatattg aagaagtgat cctcactgat atttgccctt
    24241 actcgttggg cgttgaggtt aaccgccagg gcgtttccgg cattttctcg ccgattattg
    24301 aacgaaacac cactgtgccc gtgtcgcgtg tagaaactta ttcaaccatg cacccggaac
    24361 aggattctat tacggttaac gtctatcagg gagaaaacca caaagttaaa aacaacattc
    24421 tggtggaatc cttcgatgtg ccgttgaaga gaaccggggc ttatcagtcg attgatattc
    24481 gctttagtta tgatatcaac ggtttgcttg aagttgacgt gcttctggaa gacggcagcg
    24541 ttaagtccag agtgattaac cacagcccgg taacattgag cgcgcagcag attgaagaga
    24601 gtcggacgcg gttatccgca ttgaaaattt atccgcgcga tatgctcatc aaccgcacct
    24661 ttaaggccaa actggaagag ttgtgggcgc gggcgctggg tgacgagcga gaagagattg
    24721 gccgggtgat caccgatttt gatgcggcgc tgcagtcaaa cgatatggcc cgcgtcgatg
    24781 aggtcaggcg gcaggcgagc gtttatttag ccattgagat cccataatca cggctgccgg
    24841 atgcggcgta aatgctgcat ccggcatcgg cgcaaaaaac acatcgtcaa ctaaacaaca
    24901 tcgcgatttt gccgagagca taacctatca ccagcaccgt taagctgatt ttgataatgc
    24961 tggtaatgct ctgtggtttt tcatcctcat catttttttc ttcatggttt tcaggtgttg
    25021 gctcatcgac atcttcatcc tccggctcga gttttacttt cgtcaacagc atccgggtga
    25081 taatggcctc taatggatga gcatctccgc cttccagcct tgtattaaga cgggccagca
    25141 tctcttgttt cggaaacgct tcaagtacta cctgttccgc tacccgatac aaatccagta
    25201 acggtgaatc ttcttctgtc tcgctaccga ctctcgattt ccaccacgcc catgctttgc
    25261 gtttgaagaa tagcgactca ataaacgatt gacctaacgg cgatagtttt ccgctatgta
    25321 aaatttccgc ccataacaga cgcgtccggg cgctctcacc aggaacatat ttcaacggtt
    25381 taccttgcgc atctaccatg ctccgcgctt cgataaccat cactaataac gcgaaataat
    25441 ccgggcaatg ttgtgcgctc cactgcaaca acaaattatc ggcttgtgta gatgggtaac
    25501 ttccccagta cgcgcagaga tcggtaagca ggctttcccc ctcgccgcag tacaaacgtt
    25561 gtgcacactg gtaataccgg gcatcttcgc tttctggttc ttccatttgc cactgttcgg
    25621 ctacaggaat gagttccgcc atacctgttt gcaccgagct gtaccagcgc aacagtttgc
    25681 gatgtaactt tgcatcatca ggaatgaacc acggaccttc catcgccaaa aacgcagtga
    25741 catagtcagg atggtaacgg cagcaacgtt ccagcgcgaa gtaatactca acagtctggt
    25801 tctgcaacgc aacgggatat accagaaggc caaggaaatc gaaacaatca ccagcctgaa
    25861 tggcctcaag aaaagcctcc ctttcttact catctattcc ttcgctgtca ttaaacgaat
    25921 gccagttcaa acgctctgcc agcaaagaag cacaatttaa tgaaagatat cgcgcttcta
    25981 tagcgattgc acacagcggc cagcgtaatt gatcgacatc ctccatgttc caggtattta
    26041 attgctgaat aaatttctgc caggcggagg gctgaaaacg atcactttct gaatccagta
    26101 atgccctgaa tacacgtagt atttcatgct cagtggcatc atctttttct tcatcatctg
    26161 cttcctcgac aggatttacc gccagtcgca gggcctcttc ataggcctgg cgtaatgctt
    26221 taaacccttg cggatcggtt tcgggatgac ataacggtaa gcgagcaaga taagcctggc
    26281 ggataatgtc tatttgcgtc gtgctttcaa tttcgaggat ttgccaacat gttttcatag
    26341 gtcgctcaca tccttaagcg ttaccgggca ttcaggctgg acagttaatg gctgccaggg
    26401 aagcgtggca tagaaacgat cgcttcccgc agccagctct tcgaacatcc gactcccggc
    26461 gctatcgtat ttgtacaggg ttagcgcctc gcgtagcgaa gcgttgtctt cgcattgaga
    26521 agattgccag tagagacgcc ctagtgaata ggcggaaaaa tagtgcatcc agctgtgata
    26581 gtaatggtga gctcgttcat agacgcgcga ttgcagccat aaactctcgt cttccgtcag
    26641 ccacttgttg agcacaccga cccgtgacag aaaccccatt cgaacataat cccaagcttt
    26701 gattccccct tccccacaac acatagcggt ttgggcaacg aattgggtat aaatcaaccc
    26761 tctctccgag ccaccagcaa ttaatgcttt ccactcttca ggggaagagc gatgccatgt
    26821 cagatataag ccagctaaat cattggcatg accatcatca gtcatctgat aaatagtctg
    26881 aattaaatca tcacgattat ttattcccca ggatgatgag agatcgaatt cttttatccc
    26941 ggtatacatt tttggcgagt catgcgcggg tttgcaaccc tgattaagta tttccatggg
    27001 cgcagaaagc gcaaaaagaa gatattgttc ttccttattc atttgttaat aatcccttaa
    27061 gtgcattaac tgactacttt tttattattg atgagtataa acttgacgct ttcaaaataa
    27121 aaagaaaatc gaagcattca cacatgaata aaaaattaat gtatatattc acaattttta
    27181 tagttgcagc aattacctgt attagccaac ccaagaaaac gacgttgcgt gataaagcca
    27241 tggtgaatta tgcctttgat tatttaagct caccgggcag tcttccattc accacggcag
    27301 ccacggagct ttccgcgatt catggtcact caacgtcgca atatcgcctt ggagaatttt
    27361 atcttcatgg tagcgacggt aaaccactgg attatacaca ggcgagatac tggtatgagc
    27421 aatcagcgga acaggaaaat ccacgcgcgc aaagtaaact ggggtggatc tacctcaaag
    27481 gtctgggggt caaacccgac acccgtaaag caattctctg gtataaggaa gcagctgaac
    27541 aagggtatgc tcatgctcaa tatactttag gtttgatcta cagaaatggc tcaggtatta
    27601 atgttaacca ttatgaatct caaaaatggt taaaactggc cgccaaacaa cattacaaaa
    27661 atgcggaaag attacttgcc gggcttcccg cacattaacg tcgactgtca cccgaacaga
    27721 tggagtattt tccctatcaa accagcaaag ataaaaatat agaaaataat tttaatgatg
    27781 cccagacagc ctggcccacc atcgtcaacg ggcattggct gcggttgcgg ctccggttca
    27841 acagtttgct tctcggtaaa attcgccgcc tgaattaata atcgacgaat aattaatgct
    27901 tccagtggac aacgattttc tgcatcaaga cggcttacga tcggcattaa tgattcttct
    27961 gtaggttggc tggcctgcac taattgtttg gcaactagat ataatggcca taaggtatgt
    28021 tctgtatcac gcgtgagtga gatccgcggc ttacatcctt tgtgcaaaac catctcgacc
    28081 agtgaacgcc ccagagcaga gagtttttca tcgctgagta tttcgtataa atgaaaacgc
    28141 gtacgggcgc tgtcgccggg gacataaagt agcggttttc cctggtcgtt aaccagttca
    28201 cgagcttcaa tcatcatcac cactaatggg taataatcga ctcgatgctg acggcaccat
    28261 tgcaacatta aagcatccgc ctgagtggag ggatactcgt gccagtagtc acaaagttct
    28321 gccagcaggc tttcgccttc tccacaatag acgcgttgcg cgtattcata ataacgcgca
    28381 tcttcattat caggttcagc ttcccgccac gaccgggcgt aatccaggag ttcggggatt
    28441 cccaggcgac ctgcactata ccagcggagt aaatttctgt gcagttttgc atcatcgggg
    28501 ataatcactg cgcgatgttg cgctaaatac aatgtcagcc attctggatg cgatgaccaa
    28561 atccgggcga acatttggta aaaatcgatg gtgtcatttt gcacggcaac gggcagatgc
    28621 agaatgatct ggaagttaaa aacatttccc ttcgcaatgg catataaaaa ggattccagt
    28681 tcttcttcgt ctatttcccc gacgttttcc tcctgcaacc accgcaatct ttctgctaat
    28741 aacaccacgc actcgaagga taaatgggca gtgttcatgg ctattgtgca cagcgaccag
    28801 cgtaattcat caatctcatt catcgagcaa taatttaatt gctgaatgaa tcgctgccag
    28861 gcggagggca gaaaacgttc actatcagag gcaagtaacg cacgaaaggc gagcagaatt
    28921 tcatgttctg ctacctcata ttcttctggt tgccaaacag atttagcagg cgactgcgca
    28981 atccggagcg cttcctcata cgcctgacga agttgtttaa aaccctgcgg atcggtttct
    29041 ggatgaaagg acggtaacag cgccagataa gcgcggcgga taatatcgac atcagtcgtt
    29101 tcctctatgt tgaggatctt ccagcaattt ttcataggtc gctcatatcc ttaagcgttt
    29161 ccgggcatgt ggggtagtca gccagtggtt gccagggtaa ggtcgcgtaa tatgcatcgt
    29221 cactggcaaa taattgataa aacatatcat tgccggaatc gtcgtattct ttacgcgcta
    29281 agaattcacg taataaaggc agattatcat cttcgggaga ctgccaatat tgccgaccaa
    29341 aggtataacc ggcaaagtat tgccgccagt tactataata acgtagagcc cgtagatgaa
    29401 tacgcgactg gatccataga ctttcttctt cactcaacca gttattaagt acgcccattc
    29461 gacttaaaaa acccatccgg acataatccc atgctttaat tcctccctca ccacagcatt
    29521 cggctgtgct ggcgacaaat tgcgcataag cctgaccttg ctcatttaat tcagcaagat
    29581 agtcacgcca ttcacatgga gaataacgaa accagcggtg ataaaaccct gccaaccgcg
    29641 cggcatgacc gttatcgatc attcggtgaa ttgtgcgcag caggtcatca cggtcatcaa
    29701 ttccccagga ggaagaaagg tcaaaaaagc gtgtacctaa atacattttg ggtgatgaat
    29761 gcgaaggtaa acagcattca ttgcgtattt ccatcggagt ggaaagtgca aacaatatt
//
LOCUS       DABSYT020000052        29251 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.052, whole genome shotgun sequence.
ACCESSION   DABSYT020000052 DABSYT020000000
VERSION     DABSYT020000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.052"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(144..1511)
                     /gene="rhlE"
                     /locus_tag="ID725_RS13810"
                     /old_locus_tag="pgaptmp_002762"
     CDS             complement(144..1511)
                     /gene="rhlE"
                     /locus_tag="ID725_RS13810"
                     /old_locus_tag="pgaptmp_002762"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGV7851322.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            1740..2411
                     /gene="cecR"
                     /locus_tag="ID725_RS13815"
                     /old_locus_tag="pgaptmp_002763"
     CDS             1740..2411
                     /gene="cecR"
                     /locus_tag="ID725_RS13815"
                     /old_locus_tag="pgaptmp_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGV7851323.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2414..3409
                     /gene="hlyD"
                     /locus_tag="ID725_RS13820"
                     /old_locus_tag="pgaptmp_002764"
     CDS             2414..3409
                     /gene="hlyD"
                     /locus_tag="ID725_RS13820"
                     /old_locus_tag="pgaptmp_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGV7851324.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3402..5138
                     /gene="ybhF"
                     /locus_tag="ID725_RS13825"
                     /old_locus_tag="pgaptmp_002765"
     CDS             3402..5138
                     /gene="ybhF"
                     /locus_tag="ID725_RS13825"
                     /old_locus_tag="pgaptmp_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGV7851325.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5131..6264
                     /gene="ybhS"
                     /locus_tag="ID725_RS13830"
                     /old_locus_tag="pgaptmp_002766"
     CDS             5131..6264
                     /gene="ybhS"
                     /locus_tag="ID725_RS13830"
                     /old_locus_tag="pgaptmp_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7851326.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6275..7381
                     /gene="ybhR"
                     /locus_tag="ID725_RS13835"
                     /old_locus_tag="pgaptmp_002767"
     CDS             6275..7381
                     /gene="ybhR"
                     /locus_tag="ID725_RS13835"
                     /old_locus_tag="pgaptmp_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7851327.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7343..7753)
                     /gene="ybhQ"
                     /locus_tag="ID725_RS13840"
                     /old_locus_tag="pgaptmp_002768"
     CDS             complement(7343..7753)
                     /gene="ybhQ"
                     /locus_tag="ID725_RS13840"
                     /old_locus_tag="pgaptmp_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGV7851328.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7886..8647
                     /gene="ybhP"
                     /locus_tag="ID725_RS13845"
                     /old_locus_tag="pgaptmp_002769"
     CDS             7886..8647
                     /gene="ybhP"
                     /locus_tag="ID725_RS13845"
                     /old_locus_tag="pgaptmp_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGV7851329.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNTSASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8644..9885
                     /gene="clsB"
                     /locus_tag="ID725_RS13850"
                     /old_locus_tag="pgaptmp_002770"
     CDS             8644..9885
                     /gene="clsB"
                     /locus_tag="ID725_RS13850"
                     /old_locus_tag="pgaptmp_002770"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGV7851330.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9885..10841
                     /gene="ybhN"
                     /locus_tag="ID725_RS13855"
                     /old_locus_tag="pgaptmp_002771"
     CDS             9885..10841
                     /gene="ybhN"
                     /locus_tag="ID725_RS13855"
                     /old_locus_tag="pgaptmp_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGV7851331.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10877..11590)
                     /gene="ybhM"
                     /locus_tag="ID725_RS13860"
                     /old_locus_tag="pgaptmp_002772"
     CDS             complement(10877..11590)
                     /gene="ybhM"
                     /locus_tag="ID725_RS13860"
                     /old_locus_tag="pgaptmp_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGV7851332.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11798..12502)
                     /gene="ybhL"
                     /locus_tag="ID725_RS13865"
                     /old_locus_tag="pgaptmp_002773"
     CDS             complement(11798..12502)
                     /gene="ybhL"
                     /locus_tag="ID725_RS13865"
                     /old_locus_tag="pgaptmp_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGV7851333.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12638..13090)
                     /gene="moaE"
                     /locus_tag="ID725_RS13870"
                     /old_locus_tag="pgaptmp_002774"
     CDS             complement(12638..13090)
                     /gene="moaE"
                     /locus_tag="ID725_RS13870"
                     /old_locus_tag="pgaptmp_002774"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGV7851334.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13092..13337)
                     /gene="moaD"
                     /locus_tag="ID725_RS13875"
                     /old_locus_tag="pgaptmp_002775"
     CDS             complement(13092..13337)
                     /gene="moaD"
                     /locus_tag="ID725_RS13875"
                     /old_locus_tag="pgaptmp_002775"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGV7851335.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVSGG"
     gene            complement(13330..13815)
                     /gene="moaC"
                     /locus_tag="ID725_RS13880"
                     /old_locus_tag="pgaptmp_002776"
     CDS             complement(13330..13815)
                     /gene="moaC"
                     /locus_tag="ID725_RS13880"
                     /old_locus_tag="pgaptmp_002776"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGV7851336.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13818..14330)
                     /gene="moaB"
                     /locus_tag="ID725_RS13885"
                     /old_locus_tag="pgaptmp_002777"
     CDS             complement(13818..14330)
                     /gene="moaB"
                     /locus_tag="ID725_RS13885"
                     /old_locus_tag="pgaptmp_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGV7851337.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14352..15341)
                     /gene="moaA"
                     /locus_tag="ID725_RS13890"
                     /old_locus_tag="pgaptmp_002778"
     CDS             complement(14352..15341)
                     /gene="moaA"
                     /locus_tag="ID725_RS13890"
                     /old_locus_tag="pgaptmp_002778"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGV7851338.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15329..15473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15738..16646
                     /gene="yvcK"
                     /locus_tag="ID725_RS13895"
                     /old_locus_tag="pgaptmp_002779"
     CDS             15738..16646
                     /gene="yvcK"
                     /locus_tag="ID725_RS13895"
                     /old_locus_tag="pgaptmp_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGV7851339.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16837..18858)
                     /gene="uvrB"
                     /locus_tag="ID725_RS13900"
                     /old_locus_tag="pgaptmp_002780"
     CDS             complement(16837..18858)
                     /gene="uvrB"
                     /locus_tag="ID725_RS13900"
                     /old_locus_tag="pgaptmp_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGV7851340.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19437..20114)
                     /gene="bioD"
                     /locus_tag="ID725_RS13905"
                     /old_locus_tag="pgaptmp_002781"
     CDS             complement(19437..20114)
                     /gene="bioD"
                     /locus_tag="ID725_RS13905"
                     /old_locus_tag="pgaptmp_002781"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGV7851341.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(20107..20862)
                     /gene="bioC"
                     /locus_tag="ID725_RS13910"
                     /old_locus_tag="pgaptmp_002782"
     CDS             complement(20107..20862)
                     /gene="bioC"
                     /locus_tag="ID725_RS13910"
                     /old_locus_tag="pgaptmp_002782"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGV7851342.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20849..22003)
                     /gene="bioF"
                     /locus_tag="ID725_RS13915"
                     /old_locus_tag="pgaptmp_002783"
     CDS             complement(20849..22003)
                     /gene="bioF"
                     /locus_tag="ID725_RS13915"
                     /old_locus_tag="pgaptmp_002783"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGV7851343.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(22000..23040)
                     /gene="bioB"
                     /locus_tag="ID725_RS13920"
                     /old_locus_tag="pgaptmp_002784"
     CDS             complement(22000..23040)
                     /gene="bioB"
                     /locus_tag="ID725_RS13920"
                     /old_locus_tag="pgaptmp_002784"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGV7851344.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23127..24416
                     /gene="bioA"
                     /locus_tag="ID725_RS13925"
                     /old_locus_tag="pgaptmp_002785"
     CDS             23127..24416
                     /gene="bioA"
                     /locus_tag="ID725_RS13925"
                     /old_locus_tag="pgaptmp_002785"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGV7851345.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24475..24951
                     /gene="ybhB"
                     /locus_tag="ID725_RS13930"
                     /old_locus_tag="pgaptmp_002786"
     CDS             24475..24951
                     /gene="ybhB"
                     /locus_tag="ID725_RS13930"
                     /old_locus_tag="pgaptmp_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGV7851346.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25269..25361
                     /locus_tag="ID725_RS13935"
                     /old_locus_tag="pgaptmp_002787"
                     /pseudo
     CDS             25269..25361
                     /locus_tag="ID725_RS13935"
                     /old_locus_tag="pgaptmp_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25458..26156)
                     /gene="nleD"
                     /locus_tag="ID725_RS13940"
                     /old_locus_tag="pgaptmp_002788"
     CDS             complement(25458..26156)
                     /gene="nleD"
                     /locus_tag="ID725_RS13940"
                     /old_locus_tag="pgaptmp_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HGV7851347.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(26218..26339)
                     /locus_tag="ID725_RS13945"
                     /old_locus_tag="pgaptmp_002789"
                     /pseudo
     CDS             complement(26218..26339)
                     /locus_tag="ID725_RS13945"
                     /old_locus_tag="pgaptmp_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(26387..27268)
                     /gene="nleH1-1"
                     /locus_tag="ID725_RS13950"
                     /old_locus_tag="pgaptmp_002790"
     CDS             complement(26387..27268)
                     /gene="nleH1-1"
                     /locus_tag="ID725_RS13950"
                     /old_locus_tag="pgaptmp_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HGV7851348.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNILYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(27438..27593)
                     /locus_tag="ID725_RS13955"
                     /old_locus_tag="pgaptmp_002791"
                     /pseudo
     CDS             complement(27438..27593)
                     /locus_tag="ID725_RS13955"
                     /old_locus_tag="pgaptmp_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            27872..27976
                     /locus_tag="ID725_RS13960"
                     /old_locus_tag="pgaptmp_002792"
                     /pseudo
     CDS             27872..27976
                     /locus_tag="ID725_RS13960"
                     /old_locus_tag="pgaptmp_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(28096..29115)
                     /gene="nleC"
                     /locus_tag="ID725_RS13965"
                     /old_locus_tag="pgaptmp_002793"
     CDS             complement(28096..29115)
                     /gene="nleC"
                     /locus_tag="ID725_RS13965"
                     /old_locus_tag="pgaptmp_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HGV7851349.1"
                     /translation="MICRVLDINMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEKVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEA
                     GENDNEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVD
                     KHNQQ"
     gene            complement(29149..>29251)
                     /locus_tag="ID725_RS13970"
                     /old_locus_tag="pgaptmp_002794"
     CDS             complement(29149..>29251)
                     /locus_tag="ID725_RS13970"
                     /old_locus_tag="pgaptmp_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HGV7851350.1"
                     /translation="FTPLHNYNHFCDFIEFNHPNIIMNTSQYTCSSW"
BASE COUNT         7043 a         7681 c         7332 g         7195 t
ORIGIN      
        1 aatttcattg ccattttaga tacactagat agatcataaa gcaaatttat taaaacaata
       61 aaatcagaca tgtcatgcat gtctgaccgt gttacctgaa taatgggcaa aagcctgtga
      121 tactcttttg cccttacatg gtattactgc gcagcggcag gtttacgcgg acggcgacgg
      181 cgttgttgtt cgcctgccgg tttggcatcg ccaagacgac gagatggttt ttctgcaggc
      241 ttcgcgctag ctgatttcgc accaccttct ccacggcgtg gttgctgttg accgcgacca
      301 ccaccacctt gcccacgacc gccgccgcca cgttgctggc gaccgttctg gatcggttcg
      361 gctttgattg acgggtccgg ctcataaccc ggaatcgcaa tgcgcgggat ctcttttttc
      421 agcagttttt cgatatcacg cagcagtttg tgttcatcaa cacacaccag cgacaatgct
      481 tcgccagttg ccgccgcacg gccagtacgc ccgatacggt ggacataatc ttccggtacg
      541 tttggcagtt catagttgac gacgtgcggc aactcttcga tatccagacc gcgcgcagcg
      601 atgtcagttg ccaccagtac acgaatatcg cccgatttaa aatcggccag cgcacgggta
      661 cgcgcacctt gcgatttatt accgtggatc gccgcactac ggatgccatc tttattaagc
      721 tgttcagcga gatggttagc gccatgtttg gtacgggtaa acaccagcac ctgctgccag
      781 ttccctttcc caatcatgtg cgacagcaat tcgcgcttgc gtttcttatc aacaaagtga
      841 acatgctggg tcacctgatc agacgcggta ttgcggcgtg ccacttctat ttccagcggg
      901 ttgtgcaaca gtttttccgc cagggcttta atatcgtcag agaaggtcgc ggagaacaac
      961 aggttctggc gcttcgcagg tagttttgtt aacacgcgac ggatatcgtg gataaagccc
     1021 atgtcgagca tgcggtcagc ttcatcgagg acgaggattt caacctgatc cagcttcact
     1081 gcattctgat gttccaggtc cagcaaacgt cccggcgttg ccaccagcac atcaacgccg
     1141 ccgcgcaact tcatcatttg tgggttaatg ctgacaccgc caaacaccac cagcgaacga
     1201 atgttcaggt atttgctgta atcacggacg ttttcgccaa tctgcgccgc cagttcacgg
     1261 gtcggggtaa gaatgagcgc acgtaccgga cgacgccctt tggcgtgcgg ctggcgagtg
     1321 atcaggtgtt gcaacagcgg cagcgtaaag cccgctgttt tgccggtgcc ggtctgggcg
     1381 ctagccatca ggtcgcggcc ttccagcacc gcagggatcg cctgctgctg aataggggtg
     1441 ggttcacggt aaccctgctc ggcaacggcg cgcaggatat cagggcttaa acccaaagaa
     1501 tcgaaagaca taactactcc gaaccgcccc gaccgttacc ggtgtagttt tcagggagat
     1561 actgaaaagg aaaatgacaa aaaccacaat gtcatgaggg agcggagtgt agcagttttt
     1621 gtgacgcagc gcataaatta tccctgtgac tggcgtagct aaaattctta gccatactgg
     1681 acaactccca tatggagtca taatcttaat caatcatttg attaagaatg gactgtgcga
     1741 tgaataatcc tgccatgaca atcaagggtg aacaggcgaa aaaacagctg attgctgccg
     1801 cactggcgca gtttggtgaa tatggaatga acgccaccac ccgcgagata gccgcccagg
     1861 ccgggcagaa tatcgctgcc atcacctact acttcggttc gaaagaagat ttgtacctcg
     1921 cctgcgccca gtggattgcc gattttattg gcgagcagtt ccgtccgcat gccgaggaag
     1981 ccgaacgctt gttcgcccaa ccacagcctg atcgggctgc catccgtgaa ctgatccttc
     2041 gcgcctgcag gaacatgatt aagctgctca cccaggatga caccgtcaac ctcagcaagt
     2101 ttatctcccg tgagcagctc tctcccacgg cagcctacca cctggtgcac gaacaggtga
     2161 ttagtccgct acacagccac ctgacacgcc tgattgccgc ctggaccggc tgcgacgcca
     2221 atgacacccg catgatcctt catacccatg cgttgattgg tgagattctg gcgttccgtc
     2281 ttggcaaaga aacaattctg ttacgtaccg gctggaccgc gttcgatgaa gaaaagaccg
     2341 aactgatcaa ccagacggtg acctgtcata tcgacctgat tttgcaagga ttatcgcaaa
     2401 ggagtttgta gtgatgaaaa aacctgtcgt gatcggactg gcggtagtgg tacttgccgc
     2461 cgtggttgcc ggaggctact ggtggtatca aagccgccag gataacggcc tgacgctgta
     2521 tggcaacgtg gatattcgta cggtgaatct tagtttccgt gttggggggc gcgttgaatc
     2581 gctggcggtg gacgaaggtg atgctatcaa agcgggccag gtgctgggcg aactggatca
     2641 caagccgtat gagattgccc tgatgcaggc gaaagcgggc gtctcggtgg cacaggcgca
     2701 gtatgacctg atgcttgccg ggtatcgcga tgaagaaatc gctcaggccg ccgcagcggt
     2761 gaaacaggcg caagccgcct atgactatgc gcagaacttc tataaccgcc agcaaggact
     2821 gtggaaaagc cgcaccattt cggcaaatga cctggaaaat gcccgctcct cgcgcgacca
     2881 ggcacaggca acgctgaaat cagcacagga taaattgcgt cagtaccgtt ccggtaaccg
     2941 tgaacaggac atcgctcagg cgaaagccag cctcgaacag gcgcaggcgc aactggcgca
     3001 ggcggagctg aatttacagg actcaacgtt gatagcaccg tctgatggca cgctgttaac
     3061 gcgcgcggtg gagccaggca cggttctcaa tgaaggtggc acggtgttta ccgtttcact
     3121 aacgcgtccg gtgtgggtgc gtgcttatgt tgatgaacgg aatcttgacc aggcccagcc
     3181 ggggcgcaaa gtgctgcttt ataccgatgg tcgcccggac aagccctatc acgggcagat
     3241 tggtttcgtt tcgccgactg ctgaatttac cccgaaaacc gtcgaaacac cggatctgcg
     3301 taccgacctc gtctatcgcc tgcgtattgt ggtgaccgac gccgatgatg cgttacgcca
     3361 gggaatgcca gtgacggtac aattcggtga cgaggcagga catgaatgat gccgttatca
     3421 cgctgaacgg cctggaaaaa cgctttccgg gcatggacaa gcccgccgtc gcgccgctcg
     3481 attgcaccat tcatgccggt tatgtgacgg ggctggtagg gccagacggt gcgggtaaaa
     3541 ccacgctgat gcggatgctg gcgggattac tgaaacccga cagcggcaat gccacggtga
     3601 ttggctttga tccgatcaaa aacgacggcg cgctgcacgc cgtgctcggc tatatgccgc
     3661 agaaattcgg cctgtatgaa gatctgacgg tgatggagaa cctcaatctg tacgcggatt
     3721 tgcgcagcgt caccggcgag gcacggaagc agacttttgc tcgcctgctg gagtttacgt
     3781 ctcttggccc gtttaccgga cgcctggcgg gcaagctctc cggtgggatg aaacaaaaac
     3841 tcggcctggc ctgtaccctg gtgggcgaac cgaaagtgtt gctgctcgat gaacccggcg
     3901 tcggcgttga ccctatctca cggcgcgaac tgtggcagat ggtgcatgag ctggcgggcg
     3961 aagggatgtt aatcctctgg agtacctcgt atctcgacga agccgagcag tgccgtgacg
     4021 tgttactgat gaacgaaggc gagttgctgt atcagggaga accaaaagcc ctgacacaaa
     4081 ccatggccgg acgcagcttt ctgatgacca gtccacacga gggcaaccgc aaactgttgc
     4141 aacgcgcctt gaaactgccg caggtcagcg acggcatgat tcaggggaaa tcggtacgtc
     4201 tgatcctcaa aaaagaggcc acaccagacg atattcgcca tgccgacggg atgccggaaa
     4261 tcaacatcaa cgaaactacg ccgcgttttg aagatgcgtt tattgatttg ctgggcggtg
     4321 ccggaacctc agaatcgccg ctgggtgcaa tattgcatac ggtagaaggc acacccggcg
     4381 agacggtgat cgaagcgaaa gaactgacca agaaatttgg tgattttgcc gccaccgatc
     4441 acgtcaactt tgccgttaaa cgcggggaga tttttggttt gctggggcca aacggcgcgg
     4501 gcaaatcgac cacctttaag atgatgtgcg gtttgctggt gccgacttcc ggccaggcgc
     4561 tggtgctggg gatggatctg aaagagagtt ccggtaaagc gcgccagcat ctcggctata
     4621 tggcgcaaaa attttcgctc tacggcaacc tgacggtcga acagaattta cgctttttct
     4681 ctggtgtgta tggcttacgc ggtcgggcgc agaacgaaaa aatctcccgt atgagcgagg
     4741 catttggcct gaaaagtatt gcctcacacg ccaccgatga actgccatta ggttttaaac
     4801 agcggctggc gctggcctgt tcgctgatgc atgaaccgga tattctgttt ctcgacgaac
     4861 cgacttccgg cgttgacccc ctcacccgcc gtgaattctg gctgcacatc aacagcatgg
     4921 tagagaaagg cgtcacggtg atggtcacca cccactttat ggatgaagcg gaatattgcg
     4981 accgcatcgg cctggtgtac cgcgggaaat taatcgccag cggcacgccg gacgatttga
     5041 aagcacagtc ggctaacgat gagcaacccg atcccaccat ggagcaagcc tttattcagt
     5101 tgatccacga ctgggataag gagcatagca atgagtaacc cgatcctttc ctggcgtcgc
     5161 gtgcgggcgc tgtgcgtcaa agagacgcgg cagatcgttc gcgatccgag tagctggctg
     5221 attgcggtag tgatcccgct gttgctgctg tttatttttg gttatggcat taacctcgac
     5281 tccagcaagc tgcgggtcgg gattttactg gaacagcgta gcgaagcagc gctggatttc
     5341 acccacacca tgaccggttc gccctacatc gacgccacca tcagcgataa ccgtcaggaa
     5401 ctgatcgcca aaatgcaggc ggggaaaatt cgcggtctgg tggttattcc cgtggatttc
     5461 gccgaacaga tggagcgcgc caacgctacc gcaccgattc aggtgatcac cgacggcagt
     5521 gagccgaata ccgctaactt tgtgcaggga tatgtcgaag ggatctggca gatctggcaa
     5581 atgcagcggg cggaggataa cgggcagacc tttgagccgc ttattgatgt acaaacccgc
     5641 tactggttta acccggcggc gattagccag cactttatta tccccggtgc ggtgaccatc
     5701 atcatgacgg tcatcggcgc gattctcacc tcgctggtgg tggcgcgaga atgggaacgc
     5761 ggcactatgg aggctctgct ctctacggag attacccgca cggaactgct gctgtgtaag
     5821 ctgatccctt attactttct cgggatgctg gcgatgttgc tgtgtatgct ggtgtcagtg
     5881 tttattctcg gcgtgccgta tcgcggttcg ctgctgattc tgttttttat ctccagcctg
     5941 tttttactca gtaccctggg gatggggctg ctgatttcca cgattacccg caaccagttc
     6001 aatgccgctc aggtcgccct gaacgccgcg ttcctgccgt cgattatgct ttccggcttt
     6061 atttttcaga tcgacagtat gcccgcggtg atccgcgcgg tgacgtacat tattcccgca
     6121 cgttatttcg tcagcaccct gcaaagcctg ttcctcgccg ggaatattcc agtggtgctg
     6181 gtggtgaacg tactgttttt gatcgcttcg gcggtgatgt ttatcggcct gacgtggctg
     6241 aaaaccaaac gtcggctgga ttagggagaa gagcatgttt catcgcttat ggacgttaat
     6301 ccgcaaagag ttgcagtcgt tgctgcgcga accacaaacc cgcgcgattc tgattttacc
     6361 ggtgctgatt caggtgattc tgttcccgtt cgccgccacg ctggaagtga ccaacgccac
     6421 catcgccatc tacgatgaag ataacggcga gcattcggtg gagctgaccc aacgttttgc
     6481 ccgcgccagc gcctttacac atgtgctgct gctgaaaagc ccacaggaga tccgcccgac
     6541 catcgacaca caaaaggcgt tactactggt gcgtttcccg gctgacttct cgcgcaaact
     6601 ggataccttc cagaccgcgc cgttgcagtt gatcctcgac gggcgtaact ccaacagtgc
     6661 gcaaattgcc gccaactacc tgcaacagat cgtcaaaaat tatcagcagg agctgctgga
     6721 aggaaaaccg aaacctaaca acagcgagct ggtggtacgc aactggtata acccgaatct
     6781 cgactacaaa tggtttgtgg tgccgtcgct gatcgccatg atcaccacta ttggcgtaat
     6841 gatcgtcact tcactttccg tcgcccgcga acgtgaacaa ggtacgctcg atcagctact
     6901 ggtttcgccg ctcaccacct ggcagatctt catcggcaaa gccgtaccgg cgttaattgt
     6961 cgccaccttc caggccacca ttgtgctggc gattggtatc tgggcgtatc aaatcccctt
     7021 cgccggatcg ctggcgctgt tctactttac gatggtgatt tatggtttat cgctggtggg
     7081 attcggtctg ttgatttcat cactctgttc aacacaacag caggcgttta tcggcgtgtt
     7141 tgtctttatg atgcccgcca ttctcctttc cggttacgtt tcgccggtgg aaaacatgcc
     7201 agtatggctg caaaacctga cgtggattaa tcctattcgc cactttacgg acattaccaa
     7261 gcagatttat ttgaaggatg cgagtctgga tattgtgtgg aatagtttgt ggccgctact
     7321 ggtgataacg gccacgacag ggtcagcggc gtacgcgatg tttagacgta aggtgatgta
     7381 acttcttatc tttcgccagc aaagacacta ccgccgggcc ggcaaggatt gccagccctg
     7441 caattgccag caaaaagttg ttttccagta cgcgggacaa cagccacagc acaatcagcg
     7501 ctgcgccaaa ataccaggtc gtggtcagct cttccagcac gtctgccacc tcacgccagc
     7561 gttgctcggg gtggcgctga aacaccagca tcatcactac cgtgacacaa tacagtgcgc
     7621 ataatcccac gccgacgtga accagagtct tactcatcca gcccaccacc agcagcgcca
     7681 ctaccagtaa atgcaacata atctgccagc aacttagacc cgttgcgaca cgaacacgtt
     7741 gttgccactt catggcttct ctcctgaaga atttatctct acttactcag agtaccgaac
     7801 tttacggaaa attccgtaag accgcacctt tttgtgacgc cgactacact atttcaatga
     7861 gataagaggc aaagacagga aaattatgcc cgatcaaaca caacaatttt cgttcaaggt
     7921 gctcaccatc aatattcaca aaggctttac cgcgtttaac cgacgcttca ttttgccgga
     7981 acttcgcgat gccgtgcgta ccgtcagcgc cgatattgtt tgcctgcagg aagtgatggg
     8041 cgcgcacgaa gttcatccgc tgcatgtgga aaactggccc gatacctcgc actacgagtt
     8101 tctcgccgac actatgtgga gcgattttgc ctacggtcgc aatgccgtat acccggaagg
     8161 gcatcacggc aacgccgtac tgtcgcgtta tcccattgaa cattatgaga atcgcgatgt
     8221 ttcggtcgat ggtgcggaaa agcgcggcgt gctctactgc cgcattgtgc cgccgatgac
     8281 cggaaaagcg attcatgtga tgtgcgtaca tctgggcctg cgtgaggcgc accgtcaggc
     8341 gcagcttgcg atgctcgccg aatgggtgaa tgagctaccg gacggcgaac cggtattggt
     8401 ggcgggtgat ttcaatgact ggcggcaaaa agctaatcat ccgttaaaag tgcaggccgg
     8461 actggatgag atttttaccc gcgcccacgg acgcccggcg cgcacgtttc cggtgcaatt
     8521 tcctctacta cgactggaca ggatctacgt caaaaatacc agcgccagcg cgccaaccgc
     8581 gttgccgctg cggacatggc gacacctttc tgatcatgcc cctttaagtg cggagattca
     8641 tttatgaaat gtagctggcg cgaaggcaat aagatccagt tgctggaaaa cggcgagcaa
     8701 tattatcccg cggtgtttaa ggcgattggc gaggcacaag aacgcatcat tcttgaaacg
     8761 tttatctggt ttgaggatga cgtcggcaaa caactgcatg cggcactact ggcagcagcg
     8821 caacgcgggg ttaaagcgga agtcttgctg gatggctacg gttcgccgga tctcagcgat
     8881 gagtttgtca atgaactgac ggcagctggc gtagtgttcc gctactacga tccccgccct
     8941 cgcctttttg gtatgcgcac caatgtgttt cgccggatgc atcgcaaaat tgtggtaatc
     9001 gacgcgcgta tcgcctttat tggcgggctg aattactccg ccgagcatat gtccagctac
     9061 ggtccagagg ctaaacagga ttacgcggta cgccttgaag ggccgattgt cgaagatatt
     9121 ctccagtttg agctggaaaa cctgcctgga cagagcgcgg cacgacgctg gtggcgacgt
     9181 catcacaaag cggaagagaa ccgccagccg ggagaagcgc aggtattgct ggtctggcgc
     9241 gataacgaag aacatcgcga tgatattgaa cgtcactatc tgaaaatgct cactcaggcg
     9301 cggcgagaag tcattatcgc caacgcctac ttcttccccg gctatcgatt tttacacgcc
     9361 ttgcgtaaag cggcacggcg cggggtgcgg atcaaactga tcattcaggg cgaaccggat
     9421 atgccgattg tcagagtcgg tgcgcgcttg ctgtataact atctggttaa aggcggcgtt
     9481 caggtgtttg agtaccgccg ccgcccgctc cacggcaaag tggcattgat ggacgatcac
     9541 tgggcgacag tagggtccag taatctcgat ccgctcagtt tgtcactgaa tctcgaagca
     9601 aatgtcatca tccacgatcg tcattttaac cagacgctgc gcgataatct gaacggcatt
     9661 attgccgcag attgtcagca ggtcgatgag accatgctgc cgaaacgcac ctggtggaac
     9721 ctaaccaaaa gcgtgctggc gttccacttt ttacgccact tcccggcgct ggttggctgg
     9781 cttccggcac acacgccacg tctggcgcag gttgatcctc ccgcacaacc gacaatggaa
     9841 acgcaggatc gggtagaaac tgaaaacacg ggggtaaaac cctgatgagt aaatcacacc
     9901 cgcgctggcg cttagcaaag aagatcctca cctggctgtt ttttatcgcg gtgattgtgt
     9961 tgctggtggt ctacgccaaa aaagtggact gggaagaggt ctggaaggtc atccgcgact
    10021 acaatcgcgt tgcgctgctt agtgcggtcg ggctggtggt cgtcagctat ctgatttacg
    10081 gctgctatga cctgctcgcc cgcttttact gcggtcacaa actggcgaag cgccaggtga
    10141 tgctggtgtc gtttatctgc tacgccttca acctgacgct cagtacctgg gtcggcggca
    10201 ttggtatgcg ctatcgtttg tactcccgtc tgggattgcc gggcagcact attacgcgga
    10261 ttttctcgct cagtattacc accaactggc tgggctatat tttactggca gggattatct
    10321 ttaccgcagg cgtggtggag ttgccggacc actggtatgt cgatcaaact acgctgcgta
    10381 ttctcggcat tggcttactg atgattatcg cggtttattt gtggttttgc gctttcgcga
    10441 agcaccgcca tatgaccatc aaagggcaaa agctggtgct gccttcgtgg aaattcgccc
    10501 tcgcccagat gctgatttcc agcgttaact ggatggtcat gggggcgatt atctggctgt
    10561 tacttggtca aagcgtgaac tatttctttg tactgggcgt gttgctggtt agcagtattg
    10621 ccggcgtcat cgtgcatgtt ccggcgggga ttggtgtgct ggaagcggtg tttatcgcgc
    10681 tactggctgg ggagcataca tccaaaggca caattatcgc cgccctactc gcttaccgtg
    10741 tgctgtatta ctttatcccg ctgctgctgg cgctgatctg ttacctgttg ctggaaagcc
    10801 aggcgaagaa gctacgggcg aaaaatgaag cggcgatgtg aagtttagtt aagttcttta
    10861 gtatgtgcat ttacgactaa tgaaaaaaac gcgtatgcct ttgccagaca agcgttatag
    10921 ctatcgcggc aagtatccca aagaagccga taaaatagta atagagtgtt aatgcacctg
    10981 taaaagcgca acgaaccgga ctccacaact cttcactatg gcatttcccc gccaattcaa
    11041 ggagcttatt tcgtccatgc aagataatgc ctgaccataa cacaatcatt aagcaactta
    11101 ttacccaaat gggccgttca ctcattaatg cggcattcac gatgattacc agggccaacc
    11161 ctgtcaacgt catcataatc ataaaacggt gagaaccagg atcaatatta aataataaac
    11221 cggccagcat actgattaaa aacatcgcgc ccgcagtgct gaacacgacg ataatggcat
    11281 atacaggaac taaacttgca acgacaatcg ccaccaaaga gagtaaaaac agatatatta
    11341 cgcctgcgat ggcggcattt atcttgttga tatccttaat taagccatac atacaaagta
    11401 ataataaccc tatggcgaca tacattggtg gtgaatcata tttggttgcc gtaatttcga
    11461 gatacttagc cccccaggcc agccctgcgg ttgccaccaa acctaaagcg gtaatgagcc
    11521 atataccgtt taatatcctg gtctcgtgct gaaaatctaa tttattgctg ttttgcaagt
    11581 atgattccat tcttttagtt gtccttatag aaaaatatta atattcatca tgaataaatt
    11641 taaacccacg catacaaaat acttttgtat ttatggatac ggaaaactct tccgctcggc
    11701 cataaggcgt attttatacc tggtacttgt taagtgtcgt ttttagtaag aaaaaaaatc
    11761 ccgacacatg gccgggatat tgatggtgaa aaaagaatta acgacgattg ccaaaaattc
    11821 gcagcaacat caggaacagg ttgatgaagt ccagatacaa ggttaatgcg caaagaatgg
    11881 aatatttgcg caggttcgac gcgtcgcggg tatctatctg ctcacccata tttttcagtt
    11941 tctgggtgtc atacgccgtc aatccgacaa agacaatcac gccgatgtag gtgactgccc
    12001 acatcaacgc ttcgcttttc agccagaagt tgaccagcga tgccaacaca atgccgatta
    12061 acgccataaa cagcatattg ccaaagccac ttaaatcgcg cttcgtggtg taaccgtaca
    12121 ggctcattgc gccgaacatc ccggcagtaa cgacgaaggt actggcgata gaagcagcgg
    12181 tataaacaat gaaaatactg gaaagcgtaa gacccgtcag cgccgaataa agcataaaga
    12241 gcatcgtcgt tacacctgcg ctcagctttt gaatcatcgc tgataacaca ataaccaatg
    12301 ctaattgcgc gatgatcaga ccgattaaaa agacacggtt agtgaacaac agctccatca
    12361 cggccgcgga attagccgca taccaggcaa caaatgcggt cagcaataag ccaacggtca
    12421 tccagccata gacttgcgcc atataagtct gcaagccagc ccggggttgt acaattgaat
    12481 cagaacgtgg gaatctgtcc atgacgatct cctgaagata taaggaatat cttaaggata
    12541 ctgcaaaatg atgaggctgt gcatcgacgc agcgtaaacg catgtactga gcggtgaaat
    12601 tgccggacgc agcggtgcct tatccggcta acaaaaacta ccagcgtttt gccgcctgct
    12661 gatcgctctc ccgagcttcg acccagcggt cgccctccgg cgtggcttcg cgcttccaga
    12721 acggtgcgcg ggttttgaga taatccataa taaactgccc ggcttcaaac gcactgctgc
    12781 gatgcgcact ggtgacaccg ataaaaacga tttcatcgcc cggccataat tccccgatgc
    12841 ggtgaatcac cgtgacgcgc cccagcggcc agcggttacg cgcttcatcg acaatttctg
    12901 ccagtgcttt ttcagtcatc cccggatagt gttcgagggt taatgctttg acgctgtcgc
    12961 ccaggttatg gttgcgcacc ttaccggtaa aggtgactac cgcaccgtct tcgtcacgct
    13021 ccgccagcca cgggtactct tctcctacgc tgaacggctg cggaccaaca acaattttgg
    13081 tttctgccat cttaacctcc gcttaccggc gggaagaaag ctacttcgtc gccgtcagtc
    13141 agcggatggt caaaactcac cagcgtctgg ttgacggcag ccagtaattt gccatcttcc
    13201 agcgccagcg cccagcgatc gctctgcgca gccaggtgct ggcgtaacgc ttcaacggtt
    13261 gggaaatccg cagccacttc ggttgcatct gttcctacca actcgcgcac ctgggcgaaa
    13321 aaaagaactt taatcatccg cttccacctt aaagtcaccc gacttgccgc cgctcttcgc
    13381 cagcaaacgt accggaccaa tcaccatatc tttttgcacc gctttgcaca tgtcataaat
    13441 ggtcagcgcc gccacggagg ccgcggttaa cgcttccatt tcgacaccgg ttttcccggt
    13501 caggcggcat aaggtttcta tacgcacccg attgtgctcc ggctcggcct gtaaattgac
    13561 ttcaactttg ctgagcatca gcggatgaca cagcgggatc agatcccagg tgcgttttgc
    13621 cgcctgaata ccggcaatac gcgcagtggc aaatacgtcg cctttgtggt ggcgaccatc
    13681 aataatcatc gccagcgtct cgctgcgcat ggtgacaaag gcttccgccc gcgcttcacg
    13741 cacggtttcc gctttggcgg agacatccac catgtgcgct tcgccagcgg cgttgatatg
    13801 ggtcagttgc gacatactta tttcttcaaa tgtggatgga aattacacgg acgcgtacgg
    13861 gcatccagct gcggcgcgat gatattttcc catgcggtac ggcacgcttt ggtcgaaccc
    13921 ggcatggcga aaatcagcgt tttgttagcg acgcccgcta ccgcacgaga ttgcaacgtg
    13981 gaagtgccaa tctcttcaaa cgacaacata cggaacactt caccaaaacc ttcaacttca
    14041 cggtcgaaca acggcagcaa tgcttcggga gcctgatcac cttccgtcag gccagtcccc
    14101 cccgtaatca acaccacttg tacatcgtcg ctggcgatcc acgcagatac ctgagcgcga
    14161 atagcgtagc ggttttcttt cacaatggct ttatcgacaa cgtgatggcc cgcttcttgc
    14221 gccgaatcgc gcaaatagtg accggaggta tcgtcttctt caccgcgacg attagaaacc
    14281 gtaagaatag caatacgggt cgggataaat tcagtgctta cctgactcat ctgatctctc
    14341 cttttgacgt tttagccgcc aatgtacgat aagttttgcg taataccggt gttgttttga
    14401 tgcaggaaat gggtctgttt cttctcccgc agcgccgctg aaatacgcgc ttccagcgcc
    14461 tgttgctggg tatcgtcttc cagcagatcg cgcaggttaa cgccgccttc accaaacagg
    14521 cagagatgga gtttaccaat ggaggaaacg cgcagacggt tgcaagtggc gcagaagtct
    14581 ttttcatacg gcatgataag gccaatctct ccggcgtaat ccggatgaca aaagacttgc
    14641 gcgggaccgt cgctgcgttg acgtaattgg tggatccagc cgcgacgcag tagctcgtca
    14701 cgcagaacct gaccagagat gtgatgctta cggaaaagct cgctgccctc gcccgtttcc
    14761 atcagttcga tgaaacgcag ctggataggg cgatgctgga tccagttcag aaaggtgtcg
    14821 agctggtgat gattcacatc acgcatcagc acggtattga ctttgacctt ctcaaaaccg
    14881 gcctcaaatg cagcatcgat ccctgccatc acctgattga atttatcctg gccggtaatg
    14941 gcatgaaact ggcgggcgtc cagactgtcg acgctgacgt taatgccagt aagtcccgca
    15001 tcgcgccagt tcgccacatc gcgttccaga cggtaaccat tggtggtgac cgctatctgg
    15061 cgaatagcgt cgttttctcg cacagcggcg atgatatcgg taaagtcgcg gcgtaaagac
    15121 ggctcaccgc ctgtcaggcg cactttttcg gtgcccagac tggcgaaggc gcgcgtaacc
    15181 cggcgaattt catcgacggt cagaaagcct ttattggtga cgccgctcgg tttgtagcca
    15241 tccggcaggc agtaggtgca acgaaagtta cacacatcgg taatcgacag gcgcaagtag
    15301 taaaacttac gcgcaaatgc atcagtcagt tgtgaagcca tgtacacctt tccagatacg
    15361 ggaggcgaag tcatttcttc cttcgccctg gtggcaatct ttacgagcat tgtcacggcc
    15421 aaagcaccat atcagttgac ccaggtgcag aggctagagt gtttagtggt taagccgata
    15481 ctagcgtgta aatgagattt ttaccatcca cactttcgct atgcaatcat gtatatagcg
    15541 tcctgatcgt gctatttcgc tacagaataa gggaaaatgg cggttttact ttgatataca
    15601 tcatcaggga ggcatagcgt gcatcgtcct ttagaggtag aagaaaatag tttgtatcct
    15661 caatattggc aggttaattg ctgtttcccc gcaatttgcg ctactgtagc gcggataatc
    15721 tgactccagg aaactatatg cgcaatcgta cgctggctga tcttgatcgt gtcgttgctc
    15781 tcggcggagg gcatggactg ggacgcgttc tctcatcact ttcgtctttg ggttctcgtt
    15841 taacgggtat cgtcaccacc acggataatg gtggctcgac ggggcgtatt cgccgttcag
    15901 aaggcggcat tgcctggggc gatatgcgca actgccttaa ccagctgata actgaaccca
    15961 gcgtcgcttc agcaatgttt gaatatcgtt tcggcggtaa tggcgaactt tccgggcaca
    16021 accttggaaa cctgatgttg aaggcactgg atcaccttag cgtgcgacct cttgaagcga
    16081 tcaatctgat tcgaaatctg ctgaaagtgg acgcacattt gattccgatg tcggagcatc
    16141 ctgttgatct gatggcgatt gacgatcagg ggcatgaagt ttacggcgag gtcaatatcg
    16201 accagttaac tacgccgatt caagagttat tgttaacgcc taatgtaccc gcaacgcgtg
    16261 aggcggttca cgctatcaat gaagcggatc tcatcattat tgggcctggc agtttttaca
    16321 ccagcctgat gccaattctg ctgctgaagg aaatcgcgca ggcattacgc cgcacgccag
    16381 cgccgatggt ctatatcggc aatctggggc gtgagttgag tttacctgcg gccaatttga
    16441 agctggaaag caagctggca attatggagc agtatgttgg taaaaaggtc attgatgcgg
    16501 tcatcgtcgg gccaaaagtg gatgtctcgg cggtgaaaga gcggattgtg attcaggagg
    16561 tgctggaggc cagcgatatt ccgtatcgtc atgaccgcca gttgttacat agcgcactgg
    16621 aaaaggcgtt acaggcttta ggttaacgga aatccgaagg aaaattccgg cttcctaatg
    16681 aagacaaagt gcgcgttgtt tatgacggat gcggcgtgaa cgccttatcc ggcctacaaa
    16741 ccgcgctaat tcaatgtatt gcagaatcat gtaggcctga taagcgtagc acatcaggca
    16801 attttgcttt tgtcatctgt cttcttcgtt atcctgtcac gaagccgcga taaacaattc
    16861 acgcagctga tgcaactggt cacgaatttg tgccgcttct tcgaactcca gattttgcgc
    16921 gtgttgcatc attaaccctt ccagctcatg gattttctgt tgcaacgctt taggcgacat
    16981 atccatcggc acattatccg gctcaacaat cggtcgcgat tttcctctgc ccttcgcttt
    17041 ggttttagca atgttctgcc ccaacgccag gatatcgacc actttcttgt tcaagccttg
    17101 cggcgtaatg ccgtgttcct cgttgtactt ctgctgtttc tcgcggcgac gttcggtttc
    17161 gccaatcgct ttcgccattg acggggtgat cttatcgccg tagagaatcg ctttaccgtt
    17221 aacgttacgc gccgcacgac caatagtctg gatcaacgaa cgttcagaac gcaggaagcc
    17281 ttctttgtcg gcgtcgagga tcgccaccag cgaaacctcc ggcatatcca gaccttcgcg
    17341 cagtaagttg atccccacca gcacgtcgaa ctcgcccaga cgcaagtcgc ggataatctc
    17401 catacgttcg acggtgtcga tatctgagtg aagataacgc acgcgctcac cgtgttcttc
    17461 gagatattcg gtaagatctt ccgccattcg cttggtcagt gtggtgacca gtacgcgttc
    17521 gttaattgcc gctcgctgac gaatctccga aagaagatca tcaacctgtg tcgccactgg
    17581 ccgcacttcg ataatcggat caagcaatcc ggttggacgc accacctgat ccaccacatc
    17641 gccgccggat ttttccagct cataattacc tggcgtcgcc gaaacataga tggtttgcgg
    17701 cgctaatgct tcgaactctt caaatttcag cggacggtta tccagcgctg atggcaggcg
    17761 gaagccgtac tccaccagtg tctctttacg cgcccggtca ccgcgataca tgccgccaat
    17821 ttgtggaatg gtgacgtgag attcatcgac caccagcagc ccatcggcag gcaggtaatc
    17881 aaacagcgtc ggcggtggct cacccggtcc acgaccggag aggaagcgcg agtagttttc
    17941 aatccccgaa cagtagccca gctcgttcat catctccaga tcaaactggg tacgctgggt
    18001 cagccgctgc tcttccagca gtttgttgtt ttccaatagc actttgcgtc tggcggccag
    18061 ctcttctttg atctcctcca tcgcctgtac gatgcgctcg cgcggtgtga cgtagtgcgt
    18121 tttcgggtag atggtaaaac gtggaatggt ggaaacaatc tgcccggtta gcgggtcaaa
    18181 taacgacaat cgttccactt cctcgtcaaa cagttccacg cgaagtgcaa tgtcatccga
    18241 ttctgccggg aagatatcaa tcacctcgcc acgaacacgg aaagtaccac gctggaatgc
    18301 ttgatcatta cgagcgtatt gcagctccgc cagtcggcgc agaatcgcgc gctgatcgat
    18361 aatcataccg accgtgagat ggagcatcat cttgagatat aaatcaggat cgcccagacc
    18421 ataaatcgcg gaaacagacg cgaccacaac aacatcacgc cgctccagca tcgctttggt
    18481 ggcggacaaa cgcatctgtt cgatatgttc gtttaccgag gcatctttct caatgaaggt
    18541 gtcggaactc ggtacatagg cttctggctg atagtagtcg tagtaggaga cgaaatattc
    18601 caccgcgttt tccgggaaga actctttcat ttcgccatac agctgggccg ccagcgtttt
    18661 gttgggcgca agtaccatgg tagggcgctg aaggtcagca atgacattgg caatggtgaa
    18721 ggttttcccg gaaccagtca caccaagtaa cgtctggtgc gccaggccat cttccagccc
    18781 ctcttcgaga cgtcgaatcg cctctggctg atcgccagaa ggtttaaaag cggaattcag
    18841 tttgaacggt ttactcatga gtcgctacct gaaggagttg ggcgggcagg tatgtaattt
    18901 tactcgtcgt acttaattat gccaacaaat tatactggat aaaaaaacag ttcatcacca
    18961 taatatttct gttacagcgt aaactccgct gtcaatcatg agtaaaattt actctgtggc
    19021 gagataaaac tcggacctta ccgggttatc cccaaagcaa cggctttttt aacatttgtc
    19081 aagatgagtg atgtcagttt tatggcaaga gatgcctgtt cagtgactgc cattgcaatt
    19141 atctaaccag ttaaaaaata aaagatattt ctttgagccg tctttaacgc caattcgcgt
    19201 gcaagccgcg tattctctcg cttgcctcgt gttttctaac tctaatacac atggttatcc
    19261 acaggaatag tggataactg tctgcagccc ctatcccccg ccgcctgggc aattcccaca
    19321 ttcgccgcat gatgcggaag taaaattttt ttcgtgatta ttttagaatt taattggtta
    19381 aattgcagtc aatcgaagac gcgatctcgc tcgcaattta accaaataca ggatggctac
    19441 aacaaggcaa ggtttatgta ctttccggtt gccgcatttt ctggattttc tgcaagccag
    19501 gggatctctc ccagcagcgg cgcgggaatc atgcgggtga gcgtggtcat atattcagcg
    19561 tgacgttttc ccggaggcgt gacatcgttc gccacccaac ccgccagagt cagcccggcg
    19621 tgttgtatcg cctgtgccgt caacatcgcg tgattaatac agccaagttt cacgccaacc
    19681 accagtatta ccggcagttg ttcctgtgtt acccaatccg caaaagtgaa agtgtcagaa
    19741 agcggcgtaa accagccgcc agcgccttcc actaacaccc agtcagcctg ttgttcaagc
    19801 gcgcgtaatc cggagctcat taccgatgat tctatcgacc tgccctcttg cgcgctgatg
    19861 atgtgcggtg aggtgggttc tgcgaaggtg taaggattta ccgttgcgta atccagctgc
    19921 aggctgctgt tgcgctgtaa cgccagcgcg tcactattac gtaaaccctc cggggtcttt
    19981 tcgctgccag aggcgaccgg tttataacct gccgtccgat agcctgctgc ctttgcggct
    20041 tgtaaaagtg cacaactggc gacagttttc cccacttcgg tatccgttcc ggtgacaaaa
    20101 taacgtttac tcacgaacaa tcactcccaa aaaaagatga tacgtcagag gatatcgccc
    20161 ctgttgttgc ggccaggcca gttgcaatcg ctgcaactgt gaacgcgtta atattcgctg
    20221 gtcgcgccct tcatgaagat gcgtggcacc gatgcctttc agcgaacgca tggcactgag
    20281 cgcatcatca aaccatagtg tgatgggctg aatacgatgt tgataatgca cgccgttcag
    20341 cgactgttcg atttcatctg gcggtaaaaa gcgattagca tgcgggcgct cgtccaccgc
    20401 ctgccacgcc tgatgcagtt cgggtaacga tccctgcacc agcgtggtaa acgcgaccac
    20461 gcctccgggg cgcaccaccc gatacagctc gcggagtgcc gttgataaat taccgcacca
    20521 ctgcaccgcg agattgctcc atgcaagatc gaacgtcgca gtcgctaacg gcagggattc
    20581 gatatctccc gccagataat ggtctgcagc atctttctgg cgggcttgcg caagcattgg
    20641 cggcgagaga tctaacgccg tcacctgcgc gtgacgctct cgccagtggc ggctcatcca
    20701 gccaggccca caccccgcgt ccagtacgtg ggggtattta cgctgtggga gcattgccag
    20761 taagacatca gcactctggc gctgtaactc tgcgtgttgc tcatagtgcg cggctgcccg
    20821 accaaatgcc gctgcaatgg cttgtttatt aaccgttgcc atgcagcacc tccagcagac
    20881 ggtcgatatc ctgcatttca tgcgcggcgg ttagcgttaa gcgcagtcgc gcagtaccag
    20941 agggtacggt tggcgggcga atcgccgtga cccagcagcc ttgctggcgc agtttttctg
    21001 ccagttgtaa cgcacggctg ttatcaccga cgatcaatgg ctggatggcg ctgcatgaat
    21061 cagcaagcgt aaacggcaaa tcctgtactc cggcacgaaa acgcgtaatg agtgccgcca
    21121 gtttttcgcg ccgtgcatca ccctcatcac tgcgaatgac cgccagtgac gcacgtaatg
    21181 cctgcgcctg agcgggcggc atactggtgc tgtagataag atggcgggcg aattgcagca
    21241 gataatccgc caccgtactg gagcaaagca ccgcagcccc gctgacgcca aatcctttgc
    21301 caaaagtcac taccagcaat tctggtttta ccttttgcaa ccagcaggtg ccgcgcccct
    21361 gctccccgat aacgcccgtg ccgtgggcat catcgaccat caaccagcca ttgtgctgtt
    21421 gcgttacctg ctggatttcc gccagtggcg cactatcgcc gtccatgctg aacacgcctt
    21481 ctgtcaccac cagttgctgc cccggacagg gggaagcaag cagtcgcgcc agatgagtga
    21541 catcgttatg aacaaaacgg cgaagctgcg acgggcttaa actggcagcc tccagcaatg
    21601 aggcatggct aagccggtca gcgacaatgc ggtcctcttt tgccatcatc gcggcaatga
    21661 ccgcctgatt agcggcaaaa ccagagataa atagcagtgc ccgcgaatag cccagccact
    21721 cggccagttc ttcttccagc gcctgatgcg caacgctata accgctgacg tgaccggaac
    21781 cgccgctacc gacgccaaat tgctccgcac cctgcttcca ggcacggata atttgcggat
    21841 gatggcttaa acctaaataa tcgttactgg aaaagttcag atactggcga tcgtccgcca
    21901 ccagccagcg tccggctcct tgcgccactg gataacggcg acgcagggca tcggcagcac
    21961 gccgcgcatc gagcgccgcg ttgattttct cctgccagct catagtgctg ccgcgttgta
    22021 atattcgtca gtgtccgggg tcatcagtgc ctgctccaga cgctgctgtt gttcgttatc
    22081 ccctgccagc acggcagttt gctgcggatt tagccccagt ttgcgaaaca gttgcaggtc
    22141 tttatcttct tccggattgg gcgtggttag cagtttgcaa ccgtagaaaa tcgagtttgc
    22201 gcctgccata aagcacatcg cctgagtctg ttcgttcatc tgctcgcgtc cggcagaaag
    22261 gcgcacgtaa gaggtcggca tcatgatccg cgctacggca atggtgcgaa taaaatcaaa
    22321 ggcatcgaca tcatcgttat ctgccagtgg agtacctttc accttcacca gcatgttgat
    22381 tggcacgctt tccggcggcg tcggcaggtt tgccagttgc agtaataatc cggcgcgatc
    22441 ttttaccgtt tcgcctaaac ccacaatgcc acctgagcag actttgatcc cggcatcgcg
    22501 cactttttcc agcgtatcaa ggcgttcctg ataagtacgt gtggtgatga tattgccgta
    22561 aaactccggc gaggtgtcga ggttatggtt gtagtaatcc agcccggcat tcgcaaggcg
    22621 ctgcgcctga gattcactca acgtacccag cgtcatacac gcctccagcc ccatcgcttt
    22681 tactccctgc accatttgtt ccaggtatga catatcgcgt tcgtggggat tcttccacgc
    22741 cgcgcccata cagaagcgcg tcgatcctgc cgctttcgct ttgcgcgccg actccagcac
    22801 ctgttcaact tccatcaacc gctcggcttc cagcccggtt ttgtagcgcg agctttgcgg
    22861 gcagtatttg caatcttccg gacaagctcc ggtcttaatc gacagcaagg tgctgacctg
    22921 cacctgacga ggatcgaaat gctggcgatg cacctgctgc gcttcaaaca gcagatccag
    22981 caacggtttt tcaaataatt ctgtgacttg cgacaatgtc cagcgtgggc ggtgagccat
    23041 ggggcttctc caaaacgtgt tttttgttgt taattcggtg tagacttgta aacctaaatc
    23101 ttttcaattt ggtttacaag tcgattatga caacggacga tcttgccttt gaccaacgcc
    23161 atatctggca cccatacaca tccatgacct ccccgctacc ggtttatccg gtggcgagcg
    23221 ccgaaggttg cgagctaatt ttgtctgacg gcagacgcct ggttgacggt atgtcgtcct
    23281 ggtgggcggc gatccacggt tacaatcacc cgcagcttaa tgcggcgatg aagtcgcaaa
    23341 ttgatgccat gtcgcatgtg atgtttggcg gtatcaccca tgcgccagcc attgagctgt
    23401 gccgcaaact ggtggcaatg acgccgcaac cgctggaatg cgtttttctc gcggactccg
    23461 gttccgtagc ggtggaagtg gcgatgaaaa tggcgttgca gtactggcaa gccaaaggcg
    23521 aagcgcgcca gcattttctg accttccgca atggttatca tggcgatacc tttggcgcga
    23581 tgtcggtgtg cgatccggac aactcaatgc acagcctgtg gaaaggctat ctgccagaaa
    23641 acttgtttgc tcccgccccg caaagccgca tggatggtga atgggatgag cgcgatatgg
    23701 tgggctttgc ccgcctgatg gcggcgcatc gtcatgaaat cgcagcggtg atcattgaac
    23761 cgattgtcca gggcgcaggc gggatgcgca tgtaccatcc ggaatggtta aaacgaatcc
    23821 gcaaaatgtg cgatcgcgaa ggtatcttgc tgattgccga cgagatcgcc accggatttg
    23881 gtcgtaccgg caaactgttt gcctgtgaac atgcagaaat cgcgccggac attttgtgcc
    23941 tcggtaaagc cttaaccggc ggcacaatga ccctttccgc cacactcacc acgcgcgagg
    24001 ttgcagaaac catcagtaac ggcgaagccg gctgctttat gcacgggcca acttttatgg
    24061 gcaatccgct ggcctgtgcg gctgcaaacg ccagcctggc gattctcgaa tctggcgact
    24121 ggcagcaaca ggtggcggct attgaagtac agctgcgcga gcaacttgcc cccgcccgtg
    24181 atgccgaaat ggttgccgat gtgcgcgtgc tgggggccat tggcgtagtc gaaacaactc
    24241 gtccggtgaa tatggcggca ttgcaaaaat tctttgtcga acagggtgtc tggatccggc
    24301 cttttggcaa actgatttac ctgatgccgc cctatattat tctcccgcaa cagttgcagc
    24361 gtctgaccgc agcggttaac cgcgcggtac aggatgaaac atttttttgc caataacggg
    24421 aagtcagcgt gagagtttct ggctacactt tctgcaaact agaaaggagg gtttatgaaa
    24481 ctcatcagta acgatctgcg cgatggcgat aagttgccgc atcgtcatgt ctttaacggc
    24541 atgggttacg atggcgataa tatttcaccg catctggcgt gggatgatgt tcctgcggga
    24601 acgaaaagtt ttgttgtcac ctgctacgac ccggatgcgc caaccggctc cggctggtgg
    24661 cactgggtag ttgttaattt acccgctgat acccgcgtat taccgcaagg gtttggctct
    24721 ggtctggtag caatgccaga cggcgttttg cagacgcgta ccgattttgg taaaaccgga
    24781 tacgatggcg cagcgccgcc gaaaggcgaa acccatcgct acatttttac cgttcacgcg
    24841 ctggatgtag agcgtattga tgtcgatgaa ggagccagcg gcgcgatggt cgggtttaac
    24901 gttcatttcc actctctggc aagcgcatcg attaccgcga tgtttagtta atcactctgc
    24961 cagatggcgc aatgctatct ggtatcactt aaaggtatta aaaacaactt tttgtctttt
    25021 taccttcccg tttcgctcaa gttagtataa aaaagctgaa cgcgaaacag caaaacccaa
    25081 taatatcaat gtattacaat gcatttagtc taaaaaatag actgcataat gctacaaaac
    25141 acaacatatc cagtcactat gaatcaacta cttagatggt attagtgacc tgtaacagag
    25201 cattagcgca aggtgatttt tgtcttcttg cgctaatttt ttgtcaaaca cattacgtcg
    25261 tttgctgaat ttacaaccat gctcagtatc tctataagcg cagagaaatg atgcagtggt
    25321 gggtgggctg gcttgatgaa aaggtggggt actccacctg aaccactatc ggaatagcac
    25381 aaagccttgc aatccagtgc aaagttttgt gtgcctccgt ttgccccacc aatatatatt
    25441 cagcacaaga aacacagcta aagcaatgga tgcagtctta cctgttggaa tcccagactc
    25501 actgtattgt catcatgaat aagagctgag tcgtgcgggt aggaggttct acggggcatc
    25561 ccaatctctt cgtggaattt attttctgaa agcacctcct ctgaaaaagc ccccaaccca
    25621 acagtcctgg cttcttcgag taaaagtgga gagtattttt gtgattctgc tcgggaactc
    25681 tcaactttca aacgctcccc atttaaattg tggaaaacat ggagcaactc atgaaaaaca
    25741 atagtcgcat gaaagtccac cacgctaatc ccctccccac agggatattc aactgcattc
    25801 agattacagt gaaaatcgga accagtcccc cgatggttct cagcatctat atctctatgt
    25861 gccataactc ctagtctgga agagttgagg tgtattacca ctgtttctga tttaagtcgg
    25921 gatattgatt caatagcatt caggagtaca cgccctgtct ctgtagagcc aatcttatcg
    25981 agtgctgctt caatctgctg atatatatta tgatcagtcc cctggaaacg aagttgcgga
    26041 ttatttttta tccattgaac gctcgatgct tttacaagag actcgatatc tgtatctgac
    26101 attgagctcg accttgatga ctgatgtaat accaagttga gggacgtagg gcgcatcagg
    26161 actcctgaga atgctactta ttagcagcct gtttaccact ctgtgaaaat gattttttta
    26221 aaagtgaccg tcaaggcggt caccaaaccc gataataaag cggatcatag ccatttgcca
    26281 gtcctcagcg acattgccca tttccgtgat ccgccaagaa ttgccaacca cacaatttgt
    26341 taccaactcc atccatgcaa caaatccact acactggata aaattactaa attttactta
    26401 ataccacact aataagatct tgctttccgc catgataaga ttgcattatt tgactttcac
    26461 tccatgaacg gtcggaaaca ttataagatg atatatctat tggattaaac tcattcttcg
    26521 cgcggtcata taagatattt gtctctgttg tatcgacaaa aagaattcct ttttgctcca
    26581 gtctatcaaa catatcgtaa atagcatgct cagcctgtgc tggcaaggac gaaatattta
    26641 aaagcgattc tccatttatt ttatccattc taataccaat aatatcacca ttattgccat
    26701 atattttttc tgcactcccg gcaccataat attggttgaa gcaacgaact tcgcttgtca
    26761 cctcttcatt gctttgagat gtagtaaaca tcttcaggac ttttgttgca tcctcagcat
    26821 cttcatatac tacagcgttc ccccctttgc caataacatt accaggtact gacaacctgt
    26881 tatagtccac actcggtaac tctggcggtg cataatcaac aggaggtaaa tcaggtctgt
    26941 tcgaatgaac aatgcctctt tccatagcag cgctcactgg tgatgcattc agcatgacct
    27001 caattttcct gctaatctcc cccttaggcc atcccagcct gtgcaacaga ttagtaaaac
    27061 aaccactatg actttctctt gtaacgcaaa acttattatc ggtggcaaca acacgatatg
    27121 ttctgttgcc aacctttact tgcgccccat tatcagaatg aacggcagca tcccttacag
    27181 aggataaaac acgattatca ggcgaagtca ggtttctggt taaagaattc catgaacatc
    27241 ccaaatttac agaatatggc gataacatac atttcaacct tcaaaataaa cccatctaaa
    27301 tttccttaac aaacatcccc cctaacatgg caacaaaaat tagaaatgaa ctcttgcttt
    27361 ttgtatctgg caactaattc atccttccag catcccatca aacgaacgtt tacgaactat
    27421 aacgcaggta ttaccggtca ctcgatatct ggagatacag acgtatcaac atgattgagc
    27481 ggcatacgat actgcttcca tgccgccagt aacgatgtct gctcatccgt cgcaatatct
    27541 aaatctgcag catcctgaag tggtgcgata tactcactga ttacaagtac cagaccgata
    27601 gattaaaaaa aacacaataa cacgctgcat ttaaaacaaa aaattttaaa tatgatttaa
    27661 tacaagcttt aagcctttaa ttatcagttt tatttatttt ccagcaattg attaatcaat
    27721 tgtttatata caaattattc cttttctttc tctcacgcaa aattcacaaa atattgttgc
    27781 tgcaatggac ctgtacaata gtgaatcacg gtacttttct gtgcacaacg tactgcagtt
    27841 tctttacgat ttatagctgt actgttgaat tatgtacaat ttgaatccat gcatgacgtg
    27901 tgatgcctat tgtgcatttt tccgagcctc tttttactgg gccgaagccg atgatgctga
    27961 tagtctcgtt ccggccatgt tcacttacta cagtaaaatt aggggaattg cctttacata
    28021 caatgttgca cgattaccca tcatcaactc cgttaaaatg tatgaatagt aaccttatgt
    28081 cactgcaaag acgaatcatt gctgattgtg tttgtccaca tccccaaatg taaaaatata
    28141 aggggcgcca tgatacagtt ggatctgatt tgcatcctgt aaattatcgt tgatagccaa
    28201 tccaggaaaa tcatgttgct ggataaatcc gtatcctatg ttagacacag cggaatactc
    28261 tgtgaaatca ggactcgcct catatcggtc actgatcgta cccagccttt cgaagaaagc
    28321 cctctcattc tcttcatgcc tcatggcagc ctgtcgaagg gcattcaggt tacgtagcct
    28381 aagatgagct tcacgttctg gctctgcata tcctttgaag tcgggaacac tccatcccag
    28441 ttcttgagcg acacgacgtg cgagaatctc ggtgggtcct aactctatat tactatctcc
    28501 agatggatcg ctagatccag taacatgatg aataatctcg tgaatcagtc cttcctgcca
    28561 cgatggcatc tcataagagt cagtatctgg cgcaacacta aaactgacat aaggctcttc
    28621 gttatcgttt tctcctgctt cgcaaatggg gaaaattggc tcttgcccga catcatattc
    28681 atataattcg ttacaggtta gtgattgaat atcatcaatt ttgacagaag attcttcgtt
    28741 aagctcgtat tcgtttctgt atttaatgca accaatgtgt accttctcat tatgaatgcc
    28801 atagcttacg gcatcacgaa atgtctgtga tcggctcaaa gcatcaagta cagtgttgcc
    28861 gatcatatcg actgtatgct gatcgataag cctgctacgg ctgtcatgga cggcgcgtat
    28921 tacactttcg tatacgttgc ttaaatctgc tgcggaaagt ggtattcgct tacctatatc
    28981 caaaacgtaa tccgcatagg cattttcagc acgattagga gcaatgggag cagagtatcc
    29041 tgccggggcg gaaaagttga agttggactg taatgaggga attttcatgt ttatatctaa
    29101 taccctgcaa atcatttatt acaacaaaat aaactatatc aaattcattt accatgaact
    29161 gcatgtatac tgacttgtgt tcatgattat attagggtgg ttaaactcaa taaagtcaca
    29221 aaaatgatta taattatgta atggtgtaaa a
//
LOCUS       DABSYT020000053        29137 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.053, whole genome shotgun sequence.
ACCESSION   DABSYT020000053 DABSYT020000000
VERSION     DABSYT020000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.053"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..385
                     /gene="yjfI"
                     /locus_tag="ID725_RS13975"
                     /old_locus_tag="pgaptmp_002795"
     CDS             <1..385
                     /gene="yjfI"
                     /locus_tag="ID725_RS13975"
                     /old_locus_tag="pgaptmp_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGV7851351.1"
                     /translation="ALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINILFTSRQM
                     IIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLE
                     DILLEITSLVDNALDLAEITEEYSH"
     gene            404..1102
                     /gene="yjfJ"
                     /locus_tag="ID725_RS13980"
                     /old_locus_tag="pgaptmp_002796"
     CDS             404..1102
                     /gene="yjfJ"
                     /locus_tag="ID725_RS13980"
                     /old_locus_tag="pgaptmp_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGV7851352.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1153..1812
                     /gene="yjfK"
                     /locus_tag="ID725_RS13985"
                     /old_locus_tag="pgaptmp_002797"
     CDS             1153..1812
                     /gene="yjfK"
                     /locus_tag="ID725_RS13985"
                     /old_locus_tag="pgaptmp_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGV7851353.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1830..2228
                     /gene="yjfL"
                     /locus_tag="ID725_RS13990"
                     /old_locus_tag="pgaptmp_002798"
     CDS             1830..2228
                     /gene="yjfL"
                     /locus_tag="ID725_RS13990"
                     /old_locus_tag="pgaptmp_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGV7851354.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2238..2876
                     /gene="yjfM"
                     /locus_tag="ID725_RS13995"
                     /old_locus_tag="pgaptmp_002799"
     CDS             2238..2876
                     /gene="yjfM"
                     /locus_tag="ID725_RS13995"
                     /old_locus_tag="pgaptmp_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGV7851355.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2879..4042
                     /gene="yjfC"
                     /locus_tag="ID725_RS14000"
                     /old_locus_tag="pgaptmp_002800"
     CDS             2879..4042
                     /gene="yjfC"
                     /locus_tag="ID725_RS14000"
                     /old_locus_tag="pgaptmp_002800"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGV7851356.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4126..5751
                     /gene="aidB"
                     /locus_tag="ID725_RS14005"
                     /old_locus_tag="pgaptmp_002801"
     CDS             4126..5751
                     /gene="aidB"
                     /locus_tag="ID725_RS14005"
                     /old_locus_tag="pgaptmp_002801"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGV7851357.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5868..6143)
                     /gene="yjfN"
                     /locus_tag="ID725_RS14010"
                     /old_locus_tag="pgaptmp_002802"
     CDS             complement(5868..6143)
                     /gene="yjfN"
                     /locus_tag="ID725_RS14010"
                     /old_locus_tag="pgaptmp_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGV7851358.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6292..6621)
                     /gene="bsmA"
                     /locus_tag="ID725_RS14015"
                     /old_locus_tag="pgaptmp_002803"
     CDS             complement(6292..6621)
                     /gene="bsmA"
                     /locus_tag="ID725_RS14015"
                     /old_locus_tag="pgaptmp_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGV7851359.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTLQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6803..7552
                     /gene="yjfP"
                     /locus_tag="ID725_RS14020"
                     /old_locus_tag="pgaptmp_002804"
     CDS             6803..7552
                     /gene="yjfP"
                     /locus_tag="ID725_RS14020"
                     /old_locus_tag="pgaptmp_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGV7851360.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7549..8304)
                     /gene="ulaR"
                     /locus_tag="ID725_RS14025"
                     /old_locus_tag="pgaptmp_002805"
     CDS             complement(7549..8304)
                     /gene="ulaR"
                     /locus_tag="ID725_RS14025"
                     /old_locus_tag="pgaptmp_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGV7851361.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8412..9476)
                     /gene="ulaG"
                     /locus_tag="ID725_RS14030"
                     /old_locus_tag="pgaptmp_002806"
     CDS             complement(8412..9476)
                     /gene="ulaG"
                     /locus_tag="ID725_RS14030"
                     /old_locus_tag="pgaptmp_002806"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGV7851362.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9831..11228
                     /gene="ulaA"
                     /locus_tag="ID725_RS14035"
                     /old_locus_tag="pgaptmp_002807"
     CDS             9831..11228
                     /gene="ulaA"
                     /locus_tag="ID725_RS14035"
                     /old_locus_tag="pgaptmp_002807"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGV7851363.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11244..11549
                     /gene="ulaB"
                     /locus_tag="ID725_RS14040"
                     /old_locus_tag="pgaptmp_002808"
     CDS             11244..11549
                     /gene="ulaB"
                     /locus_tag="ID725_RS14040"
                     /old_locus_tag="pgaptmp_002808"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGV7851364.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11559..12023
                     /gene="ulaC"
                     /locus_tag="ID725_RS14045"
                     /old_locus_tag="pgaptmp_002809"
     CDS             11559..12023
                     /gene="ulaC"
                     /locus_tag="ID725_RS14045"
                     /old_locus_tag="pgaptmp_002809"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGV7851365.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            12037..12687
                     /gene="ulaD"
                     /locus_tag="ID725_RS14050"
                     /old_locus_tag="pgaptmp_002810"
     CDS             12037..12687
                     /gene="ulaD"
                     /locus_tag="ID725_RS14050"
                     /old_locus_tag="pgaptmp_002810"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGV7851366.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12697..13551
                     /gene="ulaE"
                     /locus_tag="ID725_RS14055"
                     /old_locus_tag="pgaptmp_002811"
     CDS             12697..13551
                     /gene="ulaE"
                     /locus_tag="ID725_RS14055"
                     /old_locus_tag="pgaptmp_002811"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGV7851367.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAVEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13551..14237
                     /gene="ulaF"
                     /locus_tag="ID725_RS14060"
                     /old_locus_tag="pgaptmp_002812"
     CDS             13551..14237
                     /gene="ulaF"
                     /locus_tag="ID725_RS14060"
                     /old_locus_tag="pgaptmp_002812"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGV7851368.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14366..14641)
                     /gene="yjfY"
                     /locus_tag="ID725_RS14065"
                     /old_locus_tag="pgaptmp_002813"
     CDS             complement(14366..14641)
                     /gene="yjfY"
                     /locus_tag="ID725_RS14065"
                     /old_locus_tag="pgaptmp_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGV7851369.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14968..15363
                     /gene="rpsF"
                     /locus_tag="ID725_RS14070"
                     /old_locus_tag="pgaptmp_002814"
     CDS             14968..15363
                     /gene="rpsF"
                     /locus_tag="ID725_RS14070"
                     /old_locus_tag="pgaptmp_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGV7851370.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15370..15684
                     /gene="priB"
                     /locus_tag="ID725_RS14075"
                     /old_locus_tag="pgaptmp_002815"
     CDS             15370..15684
                     /gene="priB"
                     /locus_tag="ID725_RS14075"
                     /old_locus_tag="pgaptmp_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGV7851371.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15689..15916
                     /gene="rpsR"
                     /locus_tag="ID725_RS14080"
                     /old_locus_tag="pgaptmp_002816"
     CDS             15689..15916
                     /gene="rpsR"
                     /locus_tag="ID725_RS14080"
                     /old_locus_tag="pgaptmp_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGV7851372.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15958..16407
                     /gene="rplI"
                     /locus_tag="ID725_RS14085"
                     /old_locus_tag="pgaptmp_002817"
     CDS             15958..16407
                     /gene="rplI"
                     /locus_tag="ID725_RS14085"
                     /old_locus_tag="pgaptmp_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGV7851373.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(16478..17272)
                     /gene="yjfZ"
                     /locus_tag="ID725_RS14090"
                     /old_locus_tag="pgaptmp_002818"
     CDS             complement(16478..17272)
                     /gene="yjfZ"
                     /locus_tag="ID725_RS14090"
                     /old_locus_tag="pgaptmp_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGV7851374.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            17544..17837
                     /locus_tag="ID725_RS14095"
                     /old_locus_tag="pgaptmp_002819"
                     /pseudo
     CDS             17544..17837
                     /locus_tag="ID725_RS14095"
                     /old_locus_tag="pgaptmp_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(17895..18326)
                     /gene="tnpA"
                     /locus_tag="ID725_RS14100"
                     /old_locus_tag="pgaptmp_002820"
     CDS             complement(17895..18326)
                     /gene="tnpA"
                     /locus_tag="ID725_RS14100"
                     /old_locus_tag="pgaptmp_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HGV7851375.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            18334..19542
                     /locus_tag="ID725_RS14105"
                     /old_locus_tag="pgaptmp_002821"
     CDS             18334..19542
                     /locus_tag="ID725_RS14105"
                     /old_locus_tag="pgaptmp_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGV7851376.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            19586..19693
                     /locus_tag="ID725_RS14110"
                     /old_locus_tag="pgaptmp_002822"
                     /pseudo
     CDS             19586..19693
                     /locus_tag="ID725_RS14110"
                     /old_locus_tag="pgaptmp_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(19677..20315)
                     /gene="ytfB"
                     /locus_tag="ID725_RS14115"
                     /old_locus_tag="pgaptmp_002823"
     CDS             complement(19677..20315)
                     /gene="ytfB"
                     /locus_tag="ID725_RS14115"
                     /old_locus_tag="pgaptmp_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGV7851377.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            20533..21153
                     /gene="fklB"
                     /locus_tag="ID725_RS14120"
                     /old_locus_tag="pgaptmp_002824"
     CDS             20533..21153
                     /gene="fklB"
                     /locus_tag="ID725_RS14120"
                     /old_locus_tag="pgaptmp_002824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGV7851378.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            21462..22874
                     /gene="cycA"
                     /locus_tag="ID725_RS14125"
                     /old_locus_tag="pgaptmp_002825"
                     /gene_synonym="dagA"
     CDS             21462..22874
                     /gene="cycA"
                     /locus_tag="ID725_RS14125"
                     /old_locus_tag="pgaptmp_002825"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGV7851379.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(22919..23581)
                     /gene="ytfE"
                     /locus_tag="ID725_RS14130"
                     /old_locus_tag="pgaptmp_002826"
     CDS             complement(22919..23581)
                     /gene="ytfE"
                     /locus_tag="ID725_RS14130"
                     /old_locus_tag="pgaptmp_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGV7851380.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(23689..24654)
                     /gene="ytfF"
                     /locus_tag="ID725_RS14135"
                     /old_locus_tag="pgaptmp_002827"
     CDS             complement(23689..24654)
                     /gene="ytfF"
                     /locus_tag="ID725_RS14135"
                     /old_locus_tag="pgaptmp_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGV7851381.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(24762..25622)
                     /gene="qorB"
                     /locus_tag="ID725_RS14140"
                     /old_locus_tag="pgaptmp_002828"
     CDS             complement(24762..25622)
                     /gene="qorB"
                     /locus_tag="ID725_RS14140"
                     /old_locus_tag="pgaptmp_002828"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGV7851382.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            25711..26091
                     /gene="ytfH"
                     /locus_tag="ID725_RS14145"
                     /old_locus_tag="pgaptmp_002829"
     CDS             25711..26091
                     /gene="ytfH"
                     /locus_tag="ID725_RS14145"
                     /old_locus_tag="pgaptmp_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGV7851383.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(26209..28152)
                     /gene="cpdB"
                     /locus_tag="ID725_RS14150"
                     /old_locus_tag="pgaptmp_002830"
     CDS             complement(26209..28152)
                     /gene="cpdB"
                     /locus_tag="ID725_RS14150"
                     /old_locus_tag="pgaptmp_002830"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGV7851384.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            28342..29082
                     /gene="cysQ"
                     /locus_tag="ID725_RS14155"
                     /old_locus_tag="pgaptmp_002831"
     CDS             28342..29082
                     /gene="cysQ"
                     /locus_tag="ID725_RS14155"
                     /old_locus_tag="pgaptmp_002831"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGV7851385.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         6954 a         7093 c         7687 g         7403 t
ORIGIN      
        1 ggcgctggcg acggcgctgc aaactgtacc tgaacaaaat attgatgtaa caaatagcga
       61 gaacgcatta attattaaaa tgaatgatta tggcgatttg caaatcaata ttctttttac
      121 ttcccgccaa atgattatcg aaacctttat ttgtccggtg agtagtatca gcaatccaga
      181 tgaatttaat accttcttat taagaaatca gaagatgatg ccgttgtcat cggtaggaat
      241 ctccagcgta caacaagaag agtattacat tgttttcggg gcgttatcgc ttaaatcttc
      301 tcttgaagat atcctgcttg agataacctc gctggtagat aacgcattgg atctggctga
      361 aattacagaa gaatattcac actaattaca aggacgggta tttatgggaa ttttaaaaag
      421 tttatttacg ctggggaaat cgtttatctc ccaggcggaa gaatccattg aagaaaccca
      481 gggtgtgcgc atgctggaac agcatattcg tgacgctaaa gctgaactcg ataaagccgg
      541 aaaatctcgc gttgatctgc tggcgcgggt gaaattaagt cacgataagc tgaaagattt
      601 acgtgagcgc aaagccagtc tggaagcccg tacgctggaa gcgttaagca agaacgttaa
      661 tccgtcgttg attaacgaag ttgctgaaga aatcgcgcgc cttgagaatc tcattaccgc
      721 tgaagagcaa gtgttgtcga atctggaagt ctcccgtgat ggcgtggaaa aagcagttgc
      781 agcgacagcg cagcgtatcg ctcagtttga gcagcaaatg gaagtcgtta aagccactga
      841 agccatgcag cgtgcacaac aggctgtaac gacttctacc gttggcgcat cttccagcgt
      901 ttcgacagca gcagaatcct taaaacgcct gcaaacgcgt caggccgaac gtcaggctcg
      961 cctggatgct gccgcacagt tagaaaaagt cgcagacggt cgcgaccttg acgaaaagct
     1021 ggcggaagcc ggaattggcg gtagcaataa aagtagcgcc caggatgtat tagcaagact
     1081 gcaacgccaa cagggcgagt aatttttttg ccagcctcac tgcaaggctg gcaactattt
     1141 taaggataaa atatgtctgg ttttttccag cgtctgtttg gcaaggataa taagcctgct
     1201 atcgctcgcg gtccgctggg acttcatctc aatagtggct ttacgctcga cacgttagcg
     1261 tttcgtttgc tggaagatga attgctgatt gcgctgccgg gtgaggaatt tacggtagtc
     1321 gctgtcagcc acatcgatct gggcggcggt agtcagattt tccgctacta cacttcaggc
     1381 gatgagtttc tgcaaatcaa taccaccggc ggcgaagata ttgatgacat tgatgatatc
     1441 aagctctttg tctatgaaga gagctacggt atcagtaaag agaatcactg gcgcgaggcg
     1501 atcaacgcca aaacgatggg ggcaatgacc ttaaactggc aggaaaaacg ctggcagcga
     1561 ttttttaaca gcgaagaacc gggaaatatc gaacccgtct acatgctgga aaaagtagaa
     1621 aatcaaaatc atgctaaatg ggaagtccat aattttacca tgggctacca gcggcaagtg
     1681 accgaagata cttacgaata tttgctgcta aatggtgagg aatcttttaa cgatctcggc
     1741 gagccagagt ggttattttc gcgcgcgttg ggtgtcgata tcccgctgac atcacttcat
     1801 attattggtt aattacaagg acgtttacaa tgcatatact ggattcactt cttgccttta
     1861 gtgcctattt ttttattggc gtggctatgg tgattatttt tctgtttatc tactccaaaa
     1921 ttacaccgca caatgaatgg caattaatca aaaacaataa taccgcagcg tcactggcgt
     1981 tcagcggtac attgttgggt tacgtgatcc ccttatccag tgcggcaatc aatgcggtga
     2041 gtattccaga ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg
     2101 ctggtgtcag actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag
     2161 caggaatgtt tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga
     2221 catggtaatg gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc
     2281 acggagcgat cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct
     2341 acgcacaaaa atacttaacg ttggcgctaa tgggcggtgc cgcttttttt gtattgaaag
     2401 gttgtggcga tagtggcgat gtcgataacg acggcgacgg aacgttttat tcgacagtgc
     2461 aggattgtat tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca
     2521 aagcggcatt ttatgccgat gttccgaaga atatgactca gcagaactgt cagtctaagt
     2581 acgaaaattg ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt
     2641 tgttaagccg ggttattcgt aaagatcgcg atgagccgtt tgtctataac agcggcggat
     2701 cctcatttgc ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgctccg
     2761 gttctggcaa aaaagagtct tactcttcag gcggctttac caccaaaaaa gcgtcaaccg
     2821 tttctcgcgg cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat
     2881 gctgagacac aacgttcctg tgcgacggga tctggaccag atcgccgctg acaacggttt
     2941 cgactttcat atcatcgaca atgaaatcta ttgggatgag agtcgggctt accgttttac
     3001 tctgcgccag attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct
     3061 tgaggtggtg gatcgcgcgg taaaagatga agagatcctg acgcaactgg cgatcccgcc
     3121 gttgtactgg gatgtgatcg ctgaaagctg gcgcgcccgc gatccttcgc tgtatggacg
     3181 gatggatttt gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac
     3241 gccaacttca ttgtacgagt cggcttattt ccagtggctg tggctggagg atgcccggcg
     3301 cagcggcatt attccgcgtg atgccgatca gtacaatgct attcaggaac gcctgatttc
     3361 acgctttagc gagctttaca gtcgggaacc gttttatttt tgctgctgtc aggacaccga
     3421 tgaagacagg agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc
     3481 gcggtttatc tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga
     3541 tgataatgtc atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga
     3601 taacggtccg ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat
     3661 cttgagtaat aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct
     3721 tcttgcatcc tggtttgagg gtgaaaaacc gcagattgcc gctggcgaaa gctatgtgcg
     3781 taaaccgatc tactcgcgcg aaggcggaaa tgtcaccatt ttcgacggcc agaataacgt
     3841 cgttgaccac gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc
     3901 tctgccgcgg tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc
     3961 gtgcggaatg gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt
     4021 tccgcattac attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg
     4081 cagtgaccat actaatggtg actgccattg atggagggag acacagtgca ctggcaaact
     4141 cacaccgttt ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc
     4201 gcgctttgcg aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt
     4261 attggtcagc agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtgaat
     4321 ccgcctgaac tgttgcgcta cgatgcgcaa ggacgccgtc tggacgatgt gcgttttcac
     4381 cccgcctggc acctgctgat gcaggcacta tgtaccaatc gggtgcacaa tcttgcctgg
     4441 gaagaagacg ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttgcacgca
     4501 caggttgagg cggggtcgtt atgtccgata accatgacct ttgccgccac gccattgttg
     4561 ttacagatgt tacccgcgcc gtttcaggac tggaccacac cgctgttgag cgatcgctac
     4621 gattctcact tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg
     4681 gaaaagcagg gcggttccga tgtcatgagc aacaccaccc gcgcagagcg tctggaggat
     4741 ggctcttatc ggctggtggg gcataaatgg tttttctcag ttccgcaaag cgatgcgcat
     4801 ctggtgctgg cgcagaccgc gggcggtttg tcctgctttt ttgtgccgcg ctttttgcct
     4861 gacgggcaac gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct
     4921 aacgccagtt gtgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg
     4981 gaaggaattc gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt
     5041 agccatgcca tgatgcgccg tgcgttctcg ctggcgattt atcatgcgca tcaacgccat
     5101 gtttttggta atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca
     5161 cttcagcttg aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgc
     5221 gccgatgcca aagaagctct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc
     5281 tgcaaacgag gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat
     5341 tgcgaggaga gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa
     5401 ggttccggca atattatgtg cctggatgtg ctgcgcgttc tcaataagca agcgggcgta
     5461 tacgacttat tgtcggaagc atttgtagaa gtgaaagggc aggatcgcta ttttgattgc
     5521 gcggttcgtc gtttacagca gcagctgcgt aagccagctg aagaactggg gcgagagatt
     5581 actcatcagc tattcctgct gggctgcggt gcgcaaatgt tgaaatatgc ttctccgcca
     5641 atggcgcagg cgtggtgtca ggtgatgtta gatacgcgcg gcggcgtacg gttgtcagag
     5701 cagatccaga atgatttatt gctgcgggcg acggggggag tgtgtgtgta agcgtatacg
     5761 actgatgcga cgctggtttc gattaactaa atgaaatatg tgaaaattgt aggccggaca
     5821 aggcgctcgc gccgcatccg gcattgttca tttgctgtag atgacactca tgcatacagt
     5881 atcgcctgta cgcgccagtt atcaggctgc tggtcttcgt acatggtaat aatgcggtac
     5941 catgacgcac cttgttcatc ggcctttaac gccacgatac gttccacgtc ctgcggatcc
     6001 cctgaaatat tgctaactga gatctgacct atttcattca ggcccgtcac gcagtcagca
     6061 ctggcgaatt ctgcagactg tgccgtggcg ctcgcaggat aagtcactaa ttgcagcgag
     6121 ggtgaggcaa gtaattgttt catcgtcagc tccatttttc gtttccccac atgttgcagg
     6181 gcattccatc gcggtaataa acgcccaggc ttccagccgg taatttattg tgcacaggta
     6241 aacgcatagg gacgcaaagc aatgtaaagc tgactatgta agttgtctga tttatttacg
     6301 gtacaaaatg gcttgtgaat accactgtcc tgtcacgatg gtttcgtcca ccatgacgac
     6361 aacgtaataa tcagcttttg cagcgacagc ttttgctctg atttctgcta atgcgtcatc
     6421 cggagaaccc cgaaccattg tgcttacgct acctattcgc tgtaaccctt gcgtctggtc
     6481 gcggcgaatc tcttgcggat ggtccgttac tggcggtgct ggctgtagcg taccttgcag
     6541 tgcgctacag gcacttaaca tcaacaccaa taataaactg gcaaaccggt aaataacgct
     6601 attacgtttc ctgctaacca tagtgtagtg ccttattaat ttaactttgg gggaatgttc
     6661 ccgaattgtt acctggtacg cgattttcga atgaaaacgt gacgaaagcg taacccacat
     6721 ctcaacatta tgaactaata ccgccagcag aaacttgtgc taaggatttc ttcatgtcat
     6781 accagacaaa aaggagagac agatgattga aatagaatca cgcgagctgg cagatattcc
     6841 cgttcttcat gcttatcctg tcggacaaaa agataccccg ttaccgtgcg tgatttttta
     6901 tcacggcttt acttcgtcca gtctggtgta tagctatttt gccgttgcgc tggcgcaggc
     6961 tggtttgcgg gtgatcatgc cggatgcgcc cgatcacggt agccgtttta gtggtgacgc
     7021 agcgcggcgg ttaaatcaat tctggcaaat cttgctacaa agtatgcagg aattcactac
     7081 tttacgtgcg gcaatagccg aagaaaagtg gctgcttgat gaccgtctgg cagtcggtgg
     7141 tgcgtcgatg ggcgcgatga cggcactggg gattaccgct cgccacccca cggtgagatg
     7201 taccgccagc atgatgggat cgggctattt tacatcactc gcccgttcac tgtttccacc
     7261 gctgatacct gaaacggtag cacagcagaa tgaattcaat aacattgtcg cgccactggc
     7321 agagtgggaa gcgacaaacc acctggaaca acttagtgac agacctctac tgctgtggta
     7381 tggcctcgac gatgacgttg tgcctgccga cgaatcacta cgtttgcagc aggccttaag
     7441 cgagacggga cgggataaac tgctaacctg ttcatggcag ccaggcgtgc gtcaccgcat
     7501 tacgcctgag gcgttagatg ctgccgtgac atttttccgc cagcatcttt aaacacgcag
     7561 aatgctgacc ccttgcgctt ccagttgttg caggatttcc gggttagcat ttttgccggt
     7621 gataagcata tcgatttgat cggcacggct aaaaagcatt cccgcgcgtt cgccaatctt
     7681 actgctatca accagtacca ccagtttccc taccacgctc agcatcttct gctctgccat
     7741 tgctgtcagc atatcggttt tatacaaccc ttctgcggtc agcccttttc cgctggtaaa
     7801 catccagtgc ccggcataga gactgttttc gctgccctgc gggcttaaag tgatggactg
     7861 acttttgttg tactgtccgc ccataatgat cacgctgtca tgttcttgat cgatcaggta
     7921 atttgccagc ggtagataat tagtgatgat ttgcactggc ttgccacaca tttcccgccc
     7981 aagcagaaac gcggtggagc cgcagttgat gactacgctt tcgcccggat taaccagctg
     8041 cgaggccgct ttagcgatac gtactttttc atcgtgattc tgcgcctgat gcagattcat
     8101 cggcgtccag cgcgggcgct gttgggtaat agcttctgca ccattgcgca cttttttcag
     8161 tttgccgctt tcgtcaagtt tattgatatc gcgtcgcgca gtggcaggcg aaattcccag
     8221 acgctcaacg actttctcaa cggtcacaaa gcccaattgt gcgagcattt ccaggaggat
     8281 ttgatgtctt tgtgcttcag tcatgagcta ttccgataaa atttgatttt tttagatgat
     8341 atttgaaata gccaggaaat actacgccgg atagcgcgaa cttctccacg ctatccggta
     8401 taaacgacag attacaggaa tgacttgaac ggcagatccg gttcaatagt gaagcaatca
     8461 tcgaaaccgc gcggatagtg gtactcgaag ttgtctttat ccagcggcca ggtaaatttg
     8521 ccgcccacct gccagataaa cggcttgaag ccatacttca gacgatcttt tttcatctcc
     8581 cacagcacgc ggatctcttg cggatcggcc tggaagtttg accagatatc gtggtggaac
     8641 gggataacca ctttcgcatt cagcgcttca cccatacgca gcatatcggc gctggtcatc
     8701 ttgtcggtga taccgcgcgg gttttcgccg tacgatccta acgccacgtc gatctgatgt
     8761 tcgttaccgt gcttcgcata atagttagag tagtgggagt cgccgctgtg atacagggag
     8821 ccgccaggcg ttttgaacag gtagttcacc gcgcgatcgt ccatgccatc tggcagtacg
     8881 ccagccgctt tttgatcggc aggcagggtg atcagtgcag tacggtcgaa agcatcaagc
     8941 gcatgaattt caatgtcttt cacttttact acatcgcccg gtttgaccac gatgcaacgc
     9001 tctttcggta cgccccagcc aatccacaaa tccacacagg ttttcggtcc gataaacggt
     9061 acgtcatcag cacagttctg catcacggca gcagcgacgt taacgtcgat atgatcgttg
     9121 tgatcgtgag tcgccagtac cgcgtcgatc tggcgaatcg caaacggatc aagaacaaac
     9181 ggggtggtac gcaggtttgg ctgcagtttt ttcacgccag ccatgcgctg catctggtga
     9241 ccctgtttca ttaacgggtt accgtgactt tgtttgccag tgccgcacca gaaatcaacg
     9301 caaacgttgg tgccaccttc cgatttcaac cagatcccgg tgcagccaag ccaccacatc
     9361 gcaaatgtgc caggagcgac ctgttcttgt tcaatttctt cattcaacca gctaccccac
     9421 tccgggaaag tgctcaggat ccaggattca cgggtgatac ttttcacttt actcatcgcc
     9481 atttaccttc atgatagttc aattcgaatc aatatgtgat tggttttgat tgatcctgac
     9541 actatttttt ctggaaggca atgaccattt tttgactttt tgccagggaa gttgttgttg
     9601 attttgagta tgggaagatt taatggaatg tataattcaa ttaactaaat gaatttaaat
     9661 gaataattgt ttcgttgcgt gaatcccgct ctatccatgt ggaattattt gcgggtcgcg
     9721 tcacatttaa tcataaataa tcttgttgtg attacttttg aaaattagag tgagtgcaca
     9781 acattccggg tgtgtggaat acccggttac ctcttcttca ggagatcgtt atggagatcc
     9841 tctacaacat ctttaccgta ttttttaacc aggtcatgac caatgccccg ttgttgctgg
     9901 gtattgtgac ctgtctgggc tacatcctac tgcgcaaaag tgtcagcgtt attattaaag
     9961 gcacgattaa aaccataatt ggtttcatgt tgttgcaggc agggtccggc atcctcacca
    10021 gcaccttcaa accggtggtg gcgaaaatgt ccgaagtcta cggcattaac ggcgcaattt
    10081 ccgataccta cgcttcaatg atggcaacca tcgaccgcat gggcgatgcc tatagctggg
    10141 tgggttacgc cgtattgtta gcgctggcac tgaacatctg ttacgtgctg ttgcgtcgca
    10201 ttaccggcat tcgcacaatc atgttgaccg gccacatcat gttccagcag gccgggttga
    10261 ttgccgttac gctgtttatc ttcggctact ccatgtggac caccattatc tgtaccgcga
    10321 ttctggtttc gctctactgg ggcatcactt ccaacatgat gtacaagccg actcaggaag
    10381 tgacggatgg ctgtggtttc tccatcggtc accagcagca gtttgcatca tggattgcct
    10441 ataaagtcgc gccgttcctc ggcaaaaaag aggagagcgt tgaagacctc aaactgccgg
    10501 gctggctgaa cattttccac gacaacatcg tctccacggc gattgtgatg accatcttct
    10561 ttggcgccat tctgctctcc ttcggtatcg acaccgtgca ggcgatggcg ggcaaagtgc
    10621 actggacggt ctacatcctg caaaccggtt tctccttcgc ggtggcgatc ttcatcatca
    10681 ctcagggcgt gcgcatgttt gtggcggaac tctctgaagc atttaacggt atctcccagc
    10741 gcctgatccc aggtgcggtt ctggcgattg actgtgcggc tatctatagc ttcgcgccga
    10801 acgccgtggt ctggggcttt atgtggggca ccatcggtca gctgattgca gttggcatcc
    10861 tggtcgcctg cggctcttcc attctgatta ttctaggctt tatcccgatg ttcttctcca
    10921 acgccaccat cggcgtgttc gctaaccact tcggcggctg gcgtgcagcg ctgaagattt
    10981 gtctggtgat gggaatgatc gaaatctttg gttgcgtctg ggcggtgaaa ctcaccggta
    11041 tgagtgcctg gatgggcatg gcggactggt cgattctggc accgccgatg atgcaaggct
    11101 tcttctccat cggtatcgcc tttatggccg tcatcattgt aattgcactg gcttatatgt
    11161 tcttcgctgg ccgcgcgctg cgcgcagaag aagatgcaga aaaacaactg gcagaacagt
    11221 ctgcttaata aggagttttg attatgaccg tacgtattct ggctgtgtgt ggcaacggac
    11281 aaggcagttc catgatcatg aagatgaaag tggaccagtt tttaacccaa tcaaacattg
    11341 accatacggt aaacagctgc gcggttggcg agtacaaaag cgagttgagt ggcgcggata
    11401 tcatcatcgc ttctacgcac attgcgggcg aaatcaccgt gaccggcaac aaatacgtgg
    11461 ttggcgtgcg caacatgctc tctcctgccg actttggccc gaaactgctg gaagtgatca
    11521 aagagcattt cccgcaggac gtgaagtaag gacgcgctat gaaattacgt gattcgctgg
    11581 ctgaaaataa atccatccgt ctgcaggctg aagcagagac atggcaggaa gcggtgaaaa
    11641 tcggcgttga cctgctggtg gcggcagatg tggtagagcc gcgttactac caggcgattc
    11701 tggatggcgt tgaacagttc ggtccttatt tcgttatcgc tccgggcctg gcaatgccgc
    11761 acgggcgtcc ggaagagggc gttaagaaaa ccggtttctc tctggtaacg ctgaaaaagc
    11821 cgctggagtt caaccacgat gacaacgatc cggtggatat cctcatcacc atggcggcgg
    11881 tcgatgccaa tactcaccag gaagtgggca tcatgcagat cgtcaacctg tttgaagatg
    11941 aagagaattt tgaccgttta cgcgcctgcc gtaccgagca ggaagtactg gatctcattg
    12001 accgcaccaa cgcggcagct taagaaggaa ttgaacatgt cattaccgat gttgcaagtc
    12061 gcgctggaca accagactat ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa
    12121 gtcgacatta tcgaagtggg caccattctg tgcgtgggcg aaggcgtgcg tgcggttcgt
    12181 gacctgaaag cgctctaccc gcacaaaatc gtactggcag acgccaaaat tgccgatgca
    12241 ggcaaaatcc tttcgcgtat gtgcttcgaa gccaacgctg actgggtgac ggtaatttgc
    12301 tgtgcggata tcaacaccgc caaaggcgcg ctggacgtgg caaaagagtt taacggcgac
    12361 gtgcagatcg aactgaccgg ttactggacc tgggaacagg cgcaacagtg gcgcgacgca
    12421 ggtattcagc aggtggttta tcaccgcagc cgtgacgcgc aggccgcagg cgtggcgtgg
    12481 ggcgaagcgg acatcaccgc gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc
    12541 actggtggcc tggcgctgga agatctgccg ctgttcaaag gcattccgat tcacgtcttt
    12601 atcgcgggcc gtagtatccg tgatgccgct tctccggtgg aagccgcacg tcagttcaaa
    12661 cgttccatcg ccgaactgtg gggctaagga gcggatatgt tgtccaaaca aatcccgctt
    12721 ggcatctatg aaaaagcgct ccccgccggg gagtgctggc tggaacgcct gcaactggca
    12781 aaaacgttgg gcttcgattt tgtcgagatg tcggtagatg aaacggacga gcgcctgtcg
    12841 cgcctcgact ggagccgcga gcagcgtctg gcgctggtga atgcgattgt tgaaaccggc
    12901 gtgcgcgtgc cgtccatgtg cctttctgct catcgtcgtt tcccgctggg cagtgaagat
    12961 gacgcggtgc gggcgcaggg gctggagatt atgcgtaaag ctatccagtt cgcccaggac
    13021 gttggtattc gcgtgatcca actggcgggc tatgacgttt actatcagga agccaataac
    13081 gaaacgcgtc gtcgtttccg tgacggcctg aaagagagcg ttgagatggc gagccgcgcg
    13141 caggtgacgc tggcggtgga gatcatggat tatccgttga tgaactccat cagcaaggcg
    13201 ctgggctacg cgcactatct caacaatccg tggttccagc tctacccgga tatcggcaac
    13261 ctgtcggcgt gggacaacga tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc
    13321 gcggtacatg tgaaagacac caaaccgggc gtcttcaaaa acgtgccatt tggcgaaggt
    13381 gtggtggatt tcgaacgttg cttcgaaacg ctcaaacaga gtggctattg cgggccgtac
    13441 ctgattgaga tgtggagcga aacggcggaa gacccggcgg cagaagtggc gaaagcgcgt
    13501 gattgggtga aagcgcgcat ggcgaaagcg ggcatggtgg aggcggcata atgcaaaagc
    13561 taaaacagca ggtgtttgaa gccaacatgg atctgccgcg ctatgggctg gtgaccttta
    13621 cctggggcaa cgtcagcgct atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg
    13681 gcgtcgccta cgaaaccatg aaagcagacg atatggtggt ggtcgatatg agcggtaagg
    13741 tggtggaagg ggaatatcgt ccgtcttccg acaccgcgac gcatctcgaa ctctaccgtc
    13801 gttatccgtc gcttggcggc attgtccata cccactccac tcatgctacc gcatgggcgc
    13861 aggcggggct ggcgatcccg gcgttaggca ccacgcacgc tgactacttc tttggtgata
    13921 tcccgtgtac gcgtggatta agcgaagaag aggtgcaggg tgagtatgaa ctgaacactg
    13981 gcaaagtgat tatcgaaacg ctgggcaacg ccgagccgct gcatacgccg ggaattgtgg
    14041 tgtatcagca cggaccgttc gcctggggga aagatgccca cgacgcggtg cataacgcgg
    14101 tagtgatgga agaagtggcg aaaatggcgt ggattgcgcg cagtattaat ccacaactca
    14161 atcacatcga cagcttcctg atgaataaac acttcatgcg taagcacggc cccaacgctt
    14221 attacgggca gaagtagaac acgcgctgcg gaaatttcct tcctcgggag ataactggtc
    14281 taattccgca gccgtttttc aaaaaaagcc ccctgcgaag ggggcaaagc aaactatggc
    14341 aatgtttcgt tggttatacc tggtgctagc gataaatatc cgcgctggcg tgcatattgc
    14401 cgttactccc cggttcccgc atcagaatta cgtggtagta cgttgcgcct tgcgcatctg
    14461 tttcttcatt taatgcctga cgtgcttcgc tttcagtggc gaaattatga ttgatataaa
    14521 tcacgcctaa gctttgcaca tcgtccatat ttctggcctg gtggttatta atttcaatgg
    14581 ctgcccatgt atttgcactt agcaaaagca cagccagaag ggctaaaact cgactgaaca
    14641 tagatacctc ctcgacggct gactttgtgt gctctcctct gtgatgatct tctgatttaa
    14701 ttttaatcaa tgataaagaa gttgatggtg accatttctg atgcagttgt tcgaaaaaac
    14761 accatgatga agtgtgatga acttcaaatc agcgtgttag tccactttgg tgaaagatga
    14821 gatttatttg tcggctggcc ttgagtttag cggggtatgt cagtactata acgcgtcatt
    14881 tttcagccga cctttaacac gttccttgcc tccccgggat tcggctgacc cagacaggag
    14941 gctgaataat ccgtaaggag caattcgatg cgtcattacg aaatcgtttt tatggtccat
    15001 cctgaccaga gcgaacaggt tccgggcatg atcgagcgct acactgctgc catcactggt
    15061 gcagaaggca agatccaccg tctggaagac tggggccgcc gtcagctggc ttacccgatc
    15121 aacaaactgc acaaagctca ctacgttctg atgaacgttg aagctccgca ggaagtgatc
    15181 gatgagctgg aaactacctt ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt
    15241 accaagcacg ctgttaccga agcatctccg atggttaaag cgaaagacga gcgccgtgag
    15301 cgtcgcgatg atttcgcaaa cgaaaccgct gatgatgctg aagctgggga ttctgaagag
    15361 taatttctga tgaccaaccg tctggtgttg tccggcaccg tgtgcaggac tccccttcga
    15421 aaggtcagtc catcaggaat tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag
    15481 gaggaagccg gctttcaccg gcaggcatgg tgtcaaatgc ccgttattgt tagcggacac
    15541 gaaaaccagg ccattactca cagtataacg gtcggcagtc gcataaccgt tcaggggttc
    15601 atttcatgcc acaaggcaaa gaacggactg agcaaaatgg ttttgcatgc cgagcagatt
    15661 gaattgatag attctggaga ctagccatat ggcacgttat ttccgtcgtc gcaagttctg
    15721 ccgtttcacc gcggaaggcg ttcaagagat cgactataaa gatatcgcta cgctgaaaaa
    15781 ctacatcacc gaaagcggta agattgtccc aagccgtatc accggtaccc gtgcaaaata
    15841 ccagcgtcag ctggctcgcg ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac
    15901 tgatcgccat cagtaatcgg tcacggtcca ttaatacgac tttgagagga taaggtaatg
    15961 caagttattc tgcttgataa agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt
    16021 aaagcgggct atgctcgtaa cttcctggta ccacagggta aagctgttcc agctaccaag
    16081 aaaaacattg aattcttcga agcacgtcgc gctgaactgg aagctaaact ggctgaagtt
    16141 ctggcagctg ctaatgctcg cgctgagaaa atcaatgcac tggaaactgt taccatcgcg
    16201 tctaaagctg gcgacgaagg taaactgttc ggttccatcg gtactcgcga catcgctgac
    16261 gctgtaactg cagctggcgt tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt
    16321 ctgcgtacca ctggcgaaca cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa
    16381 gtgatcgtaa acgtagtagc tgaataattc gttattcaac gagacgtaaa aacgccgacc
    16441 actggtcggc gttttgcttt ctatttttcg tcaggtatta gtttcgcaag tagatccatt
    16501 caccattctc ttgtgtccat aagtcaggat ggtttgctgg cgttagatac atacgagcgc
    16561 aactgatgtc tcgctcctgc ggcactcgct cgaaacccaa acccgagtag attgcggctg
    16621 aacttgataa ggaacgcaga ccaattttca aatcatgggg taaatcagaa tacgcgttta
    16681 gcaatgcagc aatgatgaat ccgagggcat tagaaacgcc tgtaacagtg cgaagttggt
    16741 cgatataaag atcgattgtt ggtagttcag atgaccagca attaggcatt tgggcaggaa
    16801 gggttacaac gtatcctatc gtttggccgg tatagtctgg agtaccataa ccaggtccca
    16861 aaaccacttc gtgtattgtg tatttactgt tgggttcgaa ggattcgaca tcttgcaata
    16921 aatatttcag ttctgcggtt aacctcgcac ccgcgtaagc tttattgtat ttctgggcgt
    16981 gaatattatc taatgtagct tcatggttcc atccaaaaga attatcacca ttcattgtta
    17041 ctccatcgta agcattacac ttcgttgcta acttattgaa tgcttgccag tctgagagat
    17101 ctagtttgtg gatctcaaca gtacgtctgc catcgcaatt atgatctctt ccacgatttg
    17161 aaagtaatgt agcgcgtgcg tattgatgaa attttaattt tactggtttg tcggtggacg
    17221 aaaaacgaga taaatatgca ggaaatgaat aaatagtggt tggtaatgtc attgtaatgt
    17281 gacctgttta atgtttttat aaatatcgca gctgaatgtt cacagaaaaa aatcgatatt
    17341 aataaaaata agtttgtttt ctgaagtggt gaaaatagcg gatggtgaaa gatattaata
    17401 tcaaaaatcg ctcattatgg atataacgat gcacccgggg agaaaatgac ttccacgcct
    17461 tacaccgtac attcttgttg tgcggtatgt aattattcta cgctgtcggt atcggtgtat
    17521 tcgttaatca gcaggttaac cagccacaaa aagccattga gaaaattatt gattttacat
    17581 gggattatta tattgctaac ccctggtttt taaaaattgt gcatagtgaa aatcaaagta
    17641 aaggtgtgca ttatgcaaaa tcacagcgtc tcttagaaat caaccatgcg cacttgcagt
    17701 taatggaatc cttattggat gaagggaaga aatacaatat ttttaagcca gatatcgacc
    17761 cattgcaggt gaatattaat attgctgcgc ttgggggata ttatttgatc aaccagcata
    17821 cgcttggcct ggtttatccg gtgcgccgta aaaccccgtc cttcagggcg gggatatacg
    17881 gcgcggtttt ccacctaacc aggtgtttgc tgttgttcaa tgtattgacg gatggcggat
    17941 attggcgcac ctccgcagct actggcaaaa tagccaggac tccacaaaac gcctttgtag
    18001 taatacctga ctgcaatatc tggtcgatct cgtcgcagta acctacccga tacgcctttg
    18061 aggctgttta ccagactgga tatcgccagt ttgggaggat agttaattaa caaatggacg
    18121 tgatctggtt cgccatccat ttcaaccagt tcagcttcaa aatcagcaca tacctttgaa
    18181 aagtaagtgc gtagtttttc tgtcgcgtca tggtcaaaaa tctggcgtcg gtatctggtg
    18241 acaaagacca ggtgaacatg catcaggaaa acacaatgcc ttccacgccg gatatcggtt
    18301 tcttttttca cagaccaaag tatcatttgg cctgtgaaac gactacaggc atttaaattc
    18361 cagttaagac ccggtggtca acaggagcgt gaaatgaggc gcttcgcagg cgcatgtcgt
    18421 ttcgttttca atcgtgctct ggcgcttcag aatgagaatc atgaggccgg taataaatac
    18481 atcccttacg ggaaaatggc ttcctggctg gttgagtgga aaaatgccac tgaaacgcaa
    18541 tggcttaaag atgccccgtc acagccattg caacagtcac tgaaagacct tgagcgggct
    18601 tacaaaaact tcttccggaa gcgggctgct tttccccgat tcaaaaagcg gggacagaat
    18661 gatgcattcc gctacccgca gggcgttaag ctcgatcagg aaaacagccg tatttttctg
    18721 ccgaaacttg gctggatgcg ctaccggaat agccgtcagg tcacgggtgt tgtgaaaaat
    18781 gtcactgcca gccagtcctg cggtaagtgg tacatcagta ttcagactga aaatgaagta
    18841 tcaactccgg ttcacccttc agcattaatg gtcggactgg atgctggcgt ggctaaactc
    18901 gccacgctgt cagatggcac agtctttgga cctgtaaaca gttttcagaa aaaccagaag
    18961 acgctggcga gacttcagcg acagttaagc cgcaaggtca aattcagcaa caactggcag
    19021 aagcagaaac gcaaaataca gcgactgcat tcctgtatcg caaatatccg cagggactac
    19081 cttcacaaag tcacaacgac cgtcagcaaa aaccacgcaa tgatagtcat tgaggatttg
    19141 aaggtcagca acatgtcaaa gtcagcagcg ggtacggtca gccagccggg gcgcaatgtc
    19201 cgggcaaaat caggtttaaa ccgttcgata ctggatcagg gctggtatga aatgcgccgc
    19261 cagcttgagt acaagcagct ctggcgtggc ggtcaggtgc ttgctgttcc gccagcgtac
    19321 acaagccagc gttgcgcgtg ctgtggtcat acagcgaaag aaaatcgcct gtcacaaagt
    19381 aaattcagat gccaggcatg tggatataca gcgaacgctg atgtaaatgg cgctcgtaac
    19441 attttagcgg cggggcacgc cgttcttgcc tgtggggaga tggtgcagtc aggccgcccg
    19501 ttgaagcagg aacccaccga aatgattcag gcgacagcct gaacgtagca gggatccacg
    19561 tccttcaggg cttggaggat gtcaacacat cagtatggtt tcaccccagg cactggaagc
    19621 cagacgtaag gtcatcaaag agacaatcct tagctggctt ttggttgacc cttcatctac
    19681 cgcgcacgaa taaaactgcc atccggctgg cgggtgaaca ggacctgttg attattcccc
    19741 gtatcaatgg ttaagcccgt caccacgccg ctggcgttct ggcggatttt caccatctgc
    19801 ccgttttgca aattactcag cggcttaccc gcgccttcta cctgcgccat tgcatagaca
    19861 tcggtagcgg gcagtccgtg gtcgcggaac agctgcgcca tcgttttacc gggttcgaca
    19921 cggtaagagc gccattgatt atcaattccg ctatctggct ggaatggttg cgtttgggta
    19981 gttttcggct gttcttctgg ctggccttcc tgtataggtt ctggagcgac cggagccacc
    20041 tgatcaggat aattttgtgg ggtgactaat tgcgcccgca gctgttcttc tgtcggcggt
    20101 tgtgattgcg actgaatatc taactgcgct tcacgcgtca ccaccggcgc gttgggcgta
    20161 tcatcagatg gaagcagaaa tccgaccacc aatacaatgg cggcaataat gatcccccga
    20221 cgatgcatcg gcggcagcgg gtccatgaag cgaaaattgt ccggtgcgtg ccagactttc
    20281 tccagggttg gttttagttc aaagcgcccg ggcatggttt cctcctgctc cgcgtcttgt
    20341 tcctcaatca tagcctatga ataagctaac gctatgatgt ccgtggtaaa cccgccttta
    20401 ttatattcat acgggatatt gctattgttt ctttttccct gggatttgtc atcattcccg
    20461 cgacaaagtt ttacccaaag aagtgtggct gatatgctgc ccgctacttt ataccctaag
    20521 aaaggaaata cgatgaccac cccaactttt gacaccatcg aagcgcaagc aagctacggc
    20581 attggtttgc aggtagggca acaactgagt gaatctggcc tggaagggct gctgccagaa
    20641 gcactggttg caggtattgc cgatgcgctg gaaggcaaac atccggctgt tccggttgat
    20701 gtggtgcatc gcgcgctgcg tgaaatccac gagcgcgccg atgccgttcg tcgtcagcgt
    20761 ttccaggcga tggctgctga aggtgtgaaa tacctggaag aaaacgccaa aaaagaaggt
    20821 gtgaatagca ccgaatctgg cctgcaattc cgcgtgatca accagggtga aggcgcaatt
    20881 ccggcacgta ccgaccgcgt tcgtgttcat tacaccggta aactgatcga cggcaccgtg
    20941 tttgacagct ccgttgctcg tggtgaaccc gctgaatttc cggttaatgg cgtgatccct
    21001 ggctggattg aagcactgac tctgatgccg gtaggttcta aatgggaact gactatcccg
    21061 caggaactgg catatggcga gcgcggcgca ggcgcatcca tccctccgtt cagcaccctg
    21121 gtgtttgaag tcgaactgct ggaaatcctc taagcagcgc attctgttcc cctcgaacca
    21181 gaggggagca ggcattcagc aataaaccct tcagtttgcc aaacggcgct attttgtgtt
    21241 gcaaagaccc cgtaagcgtg tatttttgtg agctgtttcg cgttatcacc gtgatatgac
    21301 actcacttta aacataaaat taacattaga tctaaatctt agtattcatc ccgcgtattg
    21361 ttacctaata tcgatgagtc ccgatacaga ttcgtcgtat catagactga ctaaaggccg
    21421 tagagcctga acaacacaga caggtacagg aagaaaaaaa catggtagat caggtaaaag
    21481 tcgttgccga tgatcaggct ccggctgaac agtcgctacg gcgcaatctc acaaaccgac
    21541 atattcagct tattgccatt ggtggtgcca ttggtacggg gttgtttatg gggtctggca
    21601 aaacgattag ccttgccggg ccgtcgatca ttttcgttta tatgatcatt ggttttatgc
    21661 tctttttcgt gatgcgggca atgggggaat tgctgctttc gaatctggaa tacaaatctt
    21721 ttagtgactt cgcttccgat ttactcgggc cgtgggcagg atatttcacc ggctggactt
    21781 actggttctg ctgggttgta accggtatgg cagacgtggt ggcgatcacg gcttatgctc
    21841 agttctggtt ccccgatctc tccgactggg tcgcctcgct ggcggtgata gtgctgctgc
    21901 tgacgcttaa cctcgccact gtgaaaatgt tcggtgagat ggagttctgg tttgcgatga
    21961 tcaaaatcgt cgccattgtg tcgctgattg tcgtcggcct ggtcatggtg gcgatgcact
    22021 ttcagtcacc gactggtgtg gaagcgtcat tcgcgcattt gtggaatgac ggcggctggt
    22081 tcccgaaagg tttaagtggc ttctttgccg gattccagat agcggttttc gctttcgtgg
    22141 ggattgagct ggtaggtaca acagctgcgg aaaccaaaga tccagagaaa tcactgccac
    22201 gcgcgattaa ctccattccg atccgtatca ttatgttcta cgtcttcgcg ctgattgtta
    22261 ttatgtccgt gacgccgtgg agttcggtag tcccggagaa aagcccgttt gttgaactgt
    22321 ttgtgttggt aggtttgcct gcggctgcca gcgtgatcaa ctttgtggtg ctgacctctg
    22381 cggcgtcttc cgctaacagt ggcgtcttct ctaccagccg tatgctgttt ggtctggcgc
    22441 aggaaggtgt ggcaccgaaa gcgttcgcta aactttctaa gcgcgcagta cccgcgaaag
    22501 ggttgacttt ctcgtgtatc tgtctgctcg gcggcgtggt gatgttgtat gtgaatccta
    22561 gcgtgattgg cgcgttcacg atgattacaa ccgtttccgc gattctgttt atgttcgtct
    22621 ggacgattat cctttgctcg taccttgtgt atcgcaaaca gcgtcctcat ctacatgaga
    22681 agtcgatcta caagatgccg ctcggcaagc tgatgtgctg ggtatgtatg gcgttctttg
    22741 tgttcgtggt cgtgttgctg acactggaag atgacactcg ccaggcgctg ctggttaccc
    22801 cgctgtggtt tatcgcgctg gggctgggct ggttgtttat tggtaagaag cgggctgctg
    22861 aactgcggaa ataaccgcat tatcatgctg gatggcgcaa tgccatccag cttttagatc
    22921 actcacccgc cagcgccctt gggaacagta cattgttttc caggctgatg tgatccatca
    22981 ggtcatcaat cagttcatta atgccgttat acatcgcttt ccaggtggtg caggcttccg
    23041 gcggcggtgt gacgttattg gtattgtgtt tgatcacttc cagcagttca cccgcttcat
    23101 cgtgctcgct ttccattacg ctgattggcc ccattgcctg gctgcccatg ccttgtttga
    23161 tcatcgggaa gaggatctgc tcttctttca tcatgtggct ggaaagctct tcatgcagca
    23221 tggtcaggta ttttgtcagc ccttttggca cgctcggttt gtcggcgtga acgcgctcga
    23281 ctttagtcgc ttgcagaatc agctccggca gttgctcgcg gtgacgagcg tggtagcgca
    23341 cgatgatatg gtcgatgatt tctgccagcg gggcgctacg ccagtctttc tcaatcggtt
    23401 gttcagcaag ctttgccagc tcagcttcaa tgacctcaac atccagttct ttacgtgccg
    23461 ccgcacgcgc cagcgtttgc ttaccgccgc agcagtaatc catatcatat ttacgaaaca
    23521 gagccgaagc gcgaggaata gagagcgcca gttcacctaa aggttggtcg cgataagcca
    23581 tagctgatac ctcattctta ataagataag atgtatttta aatgcatctt taaggcaaaa
    23641 gctataaccc ttacgtagca gaggcttatt taactcactg ccagcgtgtc aactttctga
    23701 gacagattca gttaaaggtt tttctggttt tgctctaacc gcgatgacca cgccaatcac
    23761 taacagcgcg ataccgctca gcgtcattag cggcggcatt tgctggcgga gtaaaaaggt
    23821 gtacagcaaa cctgccagcg tttcgaaaac aatcagcggc ccgagaatca ctgtcggtaa
    23881 tcgctggctg gcgacgttcc agcagagtgc gccaacccat gagcaaagca cggctatcgc
    23941 aatcatcaga ctaataaaca ccagcggacg ggggccaaag ggtagggaga agtccggcgt
    24001 ttgcgtattc agccagtaac aagcgacgag atagccgata agtgaaaccg gcagtgtgac
    24061 cagcgcctgc gctgttgccc acatcatcgg atgtttgtcg ggattttccc gcagccagcg
    24121 ggcgttgcgc agggcatacc acgcccagca gaccacggaa actaacgcca gcacgatgcc
    24181 agaggtataa cgtgcccagt caaaattggg gagtccgtgg tttaactcag caatattcac
    24241 acacgccagg ccgatgccaa tacaaatcag tgccggggcg agttttcccc acgcgagttt
    24301 gccgtcgcgc tggctataaa gcagattggc gaagacggga atcaccaccg gcagggtgcc
    24361 gataatcatc gtggaaacag gcgcaccagt acgttgaatg gcactggcaa ggcagaagta
    24421 atagatgagg ttgcccatca tagtgagcat caaggcggta agccagtcgc gacgcgccaa
    24481 ctgacgcaga cgcacgcgtc ccagccaggc aatgggcagc gcaattaacc ctaacgccag
    24541 ataacgcccc atcgactgca acatcgccgg gtattccggc acgatcaacg gcccgacaaa
    24601 aataagcccc cacatcaacc ctgctaacag ggcgtacagc acgccgctaa tcattactgg
    24661 catccattga tctgtcagaa gaaagttcag gaagcagaca tgattgctcc cggcatcccg
    24721 tcattatcag agaggatgcg ggaggatgcc actttaatta actagttatt aacattaaaa
    24781 agatggctta cgctttcggc taacgttgtc gttgggtggc caatcaattt gctaagtgtt
    24841 ttgctgtcat caaacagacc gcctttcgat gcgccaacgt cagaatccgc cagcatatcc
    24901 gccagtccgt cgggcagtcc gacgcttttc agtgccgcgg cgaaatcggc ttcgctcaga
    24961 ttttgatagg taacctgttt gccgctctgt ttggtcagct ctgccgctaa ctgtgtcaac
    25021 gtccaggcac tatcgcccgc cagttcgtaa accttgcctt cgtgaccggc ttcgctaatc
    25081 acgcgtgccg cagctgccgc ataatctgcc cgcgttgctg aggcgatttt gccatcgccc
    25141 gccgcaccga taaatacgcc gtgttccagt gctgccgggg cgctggcgag gtagttttcg
    25201 gtgtaccagc cgttgcgcag tagggtgtaa acgatgccag aatcagccag cattttctcc
    25261 gtctcgatgt gctcatcggc gaggccgagc ggagatttat ccgcgtgaag caggctggta
    25321 taagcgataa atttcacgcc agccgtcttt gcggcattaa taacattacg atgctgcggg
    25381 gcacgttgac ccacttcgct ggaagagatc agcagtagtt tttccactcc ctgaagtgca
    25441 gatgtcagtg cggcttcatc gccgtagtca gcctgacgca cggtaatgcc ttgtgctgtc
    25501 agtgcctggg cttttgccgg attacgaacg atagccacta tttggctggc aggaaccgtt
    25561 ttcatcaagg attcaataac atactgacca agttggccag tggcaccagt aatagcgatc
    25621 atgggaagtc tccatcgttt tgtcttgtgt ttaaatacgc taacacctaa acttactttt
    25681 agtaagtacg cacaaaaagg taagtatgaa atgagtcagg ttagcctgtc gcaacaactg
    25741 aaagagggta atctctttgc ggaacagtgc ccgtcgcgcg aggtgttgaa acacgtcacc
    25801 agccgttggg gggtgttgat tctggtggcg ctacgcgaag gtactcatcg ctttagcgac
    25861 ctgcggcgca aaatcggcgg ggtgagcgaa aagatgcttg cgcagtcgtt acaggcgtta
    25921 gaacaggatg gttttcttaa ccgtatcgcg tatccggtgg tgccgccgca tgttgaatac
    25981 agcctcacgc cgctgggcga gcaggtgagc gaaaaggttg ctgcactggc ggactggatt
    26041 gagttgaatt tgcccgaggt gttggcggtg cgggatgaac gtgcggcata actgccggat
    26101 gcgcggccta cggtgtatga cttgtagggc ggataagatg cgccagcatc gcatccgcca
    26161 ataaatgccg gatgcgattg atgatttata ggccgaaaaa gaagtgtttt acttactcaa
    26221 atccacctga taaatcgcaa acccgatatc atcggtcgcg actttattca tcggatactg
    26281 ccctttctct ttaataaacg ctgcggcttt atctgacggg gaagtttcga aacggatatc
    26341 cagtttctta tcggcagcta tcggtgctaa acgccagttg ttatctgccg ccgggtgaat
    26401 ttcccccgca cgtttcgact catcagcaat ccacgctgcc agcaccgagc ggttctcatc
    26461 cggtgaagca aaagcgatat ggctgtcgcc cgtcccggca aatttgccgc cgtaagcgcg
    26521 atagttattg gtggcaacga ggaacatagc gttcggatca atcggcttgc cattaaaggt
    26581 caggttctta atcctttccg cattggcatt aatcatctgg cactcgccgt cataacgggc
    26641 gggctgggta acatcaatct gataattcac accatcaata acatcaaagt tataagtgcg
    26701 gaaaccatcc cagttgatga gtgactgtgg tttcgtgctg ttgggatcaa tctggttaaa
    26761 ctgtccggcg gagcactcca gccactcttt cacctcttta ccgctggctt tcaccacaat
    26821 cagcgtattg ggatagagat aaagatcggc ggcattacgg aaggtcaact ggcctttttc
    26881 cacctccaca tagcttgccg ggtcattttt gcgaccaccg actttaaacg gtgcggcagc
    26941 tgaaagcacc ggcagttttg ccagatccgg atcgccctga atgtaatgct cgacatacgc
    27001 tttttgcgcg ttgttcacta cttgcacggt cggatcgtcc tgcaccagcg ccagatagct
    27061 atacatattg tcggcagatt taccgattgg cttgctgacg aactggcgtg tggcatcgtg
    27121 atcggctttg agtgtttcta ccagcttgct gtcttccgcc gcgagggatt ttttattggc
    27181 gatgtcgtaa atcggccgtg cttccgcttt cgcctgcgtc acctgccatt taccgctgtc
    27241 attactgagt tgtaagtcga ccaccccaag atgatcgccc cacatgcccg gcattaccgc
    27301 cggaacacca ttcagcgtgc ctttggtgat atcagcccct tcgatatcag caaaatcttt
    27361 acccgggaaa acggcgtgag catggccaaa cataatggcg ttaacgcccg gaatttcact
    27421 gaggtaataa actgagtttt ccgccattac tttatacgga tcggcagaca gcccggaatg
    27481 cgccagaacg acaacgacat cggcaccttt ctcgcgcatt tcaggctcgt atttgcgcac
    27541 ggtttcggta atatcattca ccgtcacttt tccggataaa ttagctttat cccagcccat
    27601 gatttgcggc ggcacgacgc caatatagcc aatcttcagc gtctgttttt ttccgtcttt
    27661 atcgaccact tcggtgtctt taattaaata cggtgtaaac attggctgtt tggttctggc
    27721 gtcaatgacg ttggcattta cataagggaa tttcgctcct gccagcgcat ttttcaggta
    27781 atccagaccg tagttaaact cgtggttgcc aagcgttccg acggtatagt ccagcgtatt
    27841 taatgcctta tagaccgggt gaatatcacc tgcttttaat cctttcgccg acatgtaatc
    27901 ggccagcgga ctcccctgaa tcaaatcgcc gttatcgacc agtacgctgt ttttcacttc
    27961 attgcgggca ttgttaatca ggcttgccgt acgtaccagt ccgaattttt ccgtggcggt
    28021 gtctttgtaa taatcgaaat ccatcatgtt gctatgcaga tcagtggttt ccatgatacg
    28081 cagatcgacc gtcgctgcat tcacactggc ggcaatcagc gtggccagga gcgttgcgct
    28141 aaacttaatc atcagggaca tccttttatc atcgggaata cgaaagaaaa gggagaataa
    28201 acgtcttact tatagaacag tgaagaatgc cacaatttta cgctttgaaa atgatgacac
    28261 tatcacagtt ggcgcattca ttaatgatag ggtataagta aaacaataag ttaacaccgc
    28321 tcacagagac gaggtggaga aatgttagat caagtatgcc agcttgcacg gaatgcaggc
    28381 gatgccatta tgcaggtcta cgacggggcg aaaccgatgg acgtcgtcag caaagcggac
    28441 aattctccgg taacggcagc ggatattgcc gctcacaccg ttatcatgga cggtttacgt
    28501 acgctgacac cggatattcc ggtcctttct gaagaagatc ctcccggttg ggaagtccgt
    28561 cagcactggc agcgttactg gctggtagac ccgctggatg gtacgaaaga gtttattaaa
    28621 cgtaatggcg aattcaccgt taacattgcg ctcattgacc atggcaaacc gattttaggt
    28681 gtggtgtatg cgccggtaat gaacgtaatg tacagcgcgg cagaaggcaa ggcctggaaa
    28741 gaagagtgcg gcgtgcgcaa gcagattcag gtccgcgatg cgcgcccgcc gctggtggtg
    28801 atcagccgct ctcatgcgga tgcggagctg aaagagtatc tgcaacagct tggcgaacat
    28861 cagaccacgt ccatcggctc ttcgctgaag ttctgcctgg tggcggaagg acaggcgcag
    28921 ctgtacccgc gtttcggacc aacgaatatt tgggacaccg ccgctggaca tgctgtggct
    28981 gcagctgccg gagcgcacgt tcacgactgg cagggtaaac cgctggatta cactccgcgc
    29041 gagtcgttcc tgaatccggg gttcagagtg tctatttact aaattcagat ggcagaaaca
    29101 gtgtatttcc tgattctgcc atcctgattt ctcccaa
//
LOCUS       DABSYT020000054        28801 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.054, whole genome shotgun sequence.
ACCESSION   DABSYT020000054 DABSYT020000000
VERSION     DABSYT020000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.054"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            87..1040
                     /gene="ybhD"
                     /locus_tag="ID725_RS14160"
                     /old_locus_tag="pgaptmp_002832"
     CDS             87..1040
                     /gene="ybhD"
                     /locus_tag="ID725_RS14160"
                     /old_locus_tag="pgaptmp_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7851386.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1081..2076)
                     /gene="pgl"
                     /locus_tag="ID725_RS14165"
                     /old_locus_tag="pgaptmp_002833"
     CDS             complement(1081..2076)
                     /gene="pgl"
                     /locus_tag="ID725_RS14165"
                     /old_locus_tag="pgaptmp_002833"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGV7851387.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2231..3049
                     /gene="ybhA"
                     /locus_tag="ID725_RS14170"
                     /old_locus_tag="pgaptmp_002834"
     CDS             2231..3049
                     /gene="ybhA"
                     /locus_tag="ID725_RS14170"
                     /old_locus_tag="pgaptmp_002834"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGV7851388.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3050..4108)
                     /gene="modC"
                     /locus_tag="ID725_RS14175"
                     /old_locus_tag="pgaptmp_002835"
     CDS             complement(3050..4108)
                     /gene="modC"
                     /locus_tag="ID725_RS14175"
                     /old_locus_tag="pgaptmp_002835"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGV7851389.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4111..4800)
                     /gene="modB"
                     /locus_tag="ID725_RS14180"
                     /old_locus_tag="pgaptmp_002836"
     CDS             complement(4111..4800)
                     /gene="modB"
                     /locus_tag="ID725_RS14180"
                     /old_locus_tag="pgaptmp_002836"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGV7851390.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4800..5573)
                     /gene="modA"
                     /locus_tag="ID725_RS14185"
                     /old_locus_tag="pgaptmp_002837"
     CDS             complement(4800..5573)
                     /gene="modA"
                     /locus_tag="ID725_RS14185"
                     /old_locus_tag="pgaptmp_002837"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGV7851391.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5740..5889)
                     /gene="acrZ"
                     /locus_tag="ID725_RS14190"
                     /old_locus_tag="pgaptmp_002838"
     CDS             complement(5740..5889)
                     /gene="acrZ"
                     /locus_tag="ID725_RS14190"
                     /old_locus_tag="pgaptmp_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGV7851392.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6018..6806
                     /gene="modE"
                     /locus_tag="ID725_RS14195"
                     /old_locus_tag="pgaptmp_002839"
     CDS             6018..6806
                     /gene="modE"
                     /locus_tag="ID725_RS14195"
                     /old_locus_tag="pgaptmp_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGV7851393.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6874..8346
                     /gene="modF"
                     /locus_tag="ID725_RS14200"
                     /old_locus_tag="pgaptmp_002840"
     CDS             6874..8346
                     /gene="modF"
                     /locus_tag="ID725_RS14200"
                     /old_locus_tag="pgaptmp_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGV7851394.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            8608..9624
                     /gene="galE"
                     /locus_tag="ID725_RS14205"
                     /old_locus_tag="pgaptmp_002841"
     CDS             8608..9624
                     /gene="galE"
                     /locus_tag="ID725_RS14205"
                     /old_locus_tag="pgaptmp_002841"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGV7851395.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SRACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9634..10680
                     /gene="galT"
                     /locus_tag="ID725_RS14210"
                     /old_locus_tag="pgaptmp_002842"
     CDS             9634..10680
                     /gene="galT"
                     /locus_tag="ID725_RS14210"
                     /old_locus_tag="pgaptmp_002842"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGV7851396.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10684..11832
                     /gene="galK"
                     /locus_tag="ID725_RS14215"
                     /old_locus_tag="pgaptmp_002843"
     CDS             10684..11832
                     /gene="galK"
                     /locus_tag="ID725_RS14215"
                     /old_locus_tag="pgaptmp_002843"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGV7851397.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11826..12866
                     /gene="galM"
                     /locus_tag="ID725_RS14220"
                     /old_locus_tag="pgaptmp_002844"
     CDS             11826..12866
                     /gene="galM"
                     /locus_tag="ID725_RS14220"
                     /old_locus_tag="pgaptmp_002844"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGV7851398.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            13068..13820
                     /gene="gpmA"
                     /locus_tag="ID725_RS14225"
                     /old_locus_tag="pgaptmp_002845"
     CDS             13068..13820
                     /gene="gpmA"
                     /locus_tag="ID725_RS14225"
                     /old_locus_tag="pgaptmp_002845"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGV7851399.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13986..15038)
                     /gene="aroG"
                     /locus_tag="ID725_RS14230"
                     /old_locus_tag="pgaptmp_002846"
     CDS             complement(13986..15038)
                     /gene="aroG"
                     /locus_tag="ID725_RS14230"
                     /old_locus_tag="pgaptmp_002846"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGV7851400.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15354..15734
                     /gene="ybgS"
                     /locus_tag="ID725_RS14235"
                     /old_locus_tag="pgaptmp_002847"
     CDS             15354..15734
                     /gene="ybgS"
                     /locus_tag="ID725_RS14235"
                     /old_locus_tag="pgaptmp_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGV7851401.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15848..16789
                     /gene="zitB"
                     /locus_tag="ID725_RS14240"
                     /old_locus_tag="pgaptmp_002848"
     CDS             15848..16789
                     /gene="zitB"
                     /locus_tag="ID725_RS14240"
                     /old_locus_tag="pgaptmp_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGV7851402.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16786..17505)
                     /gene="pnuC"
                     /locus_tag="ID725_RS14245"
                     /old_locus_tag="pgaptmp_002849"
     CDS             complement(16786..17505)
                     /gene="pnuC"
                     /locus_tag="ID725_RS14245"
                     /old_locus_tag="pgaptmp_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGV7851403.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17543..18586)
                     /gene="nadA"
                     /locus_tag="ID725_RS14250"
                     /old_locus_tag="pgaptmp_002850"
     CDS             complement(17543..18586)
                     /gene="nadA"
                     /locus_tag="ID725_RS14250"
                     /old_locus_tag="pgaptmp_002850"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGV7851404.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18864..18939)
                     /locus_tag="ID725_RS14255"
                     /old_locus_tag="pgaptmp_002851"
     tRNA            complement(18864..18939)
                     /locus_tag="ID725_RS14255"
                     /old_locus_tag="pgaptmp_002851"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18904..18906),aa:Lys,seq:ttt)
     gene            complement(19086..19161)
                     /locus_tag="ID725_RS14260"
                     /old_locus_tag="pgaptmp_002852"
     tRNA            complement(19086..19161)
                     /locus_tag="ID725_RS14260"
                     /old_locus_tag="pgaptmp_002852"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19126..19128),aa:Lys,seq:ttt)
     gene            complement(19165..19240)
                     /locus_tag="ID725_RS14265"
                     /old_locus_tag="pgaptmp_002853"
     tRNA            complement(19165..19240)
                     /locus_tag="ID725_RS14265"
                     /old_locus_tag="pgaptmp_002853"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19205..19207),aa:Val,seq:tac)
     gene            complement(19292..19367)
                     /locus_tag="ID725_RS14270"
                     /old_locus_tag="pgaptmp_002854"
     tRNA            complement(19292..19367)
                     /locus_tag="ID725_RS14270"
                     /old_locus_tag="pgaptmp_002854"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19332..19334),aa:Lys,seq:ttt)
     gene            complement(19370..19445)
                     /locus_tag="ID725_RS14275"
                     /old_locus_tag="pgaptmp_002855"
     tRNA            complement(19370..19445)
                     /locus_tag="ID725_RS14275"
                     /old_locus_tag="pgaptmp_002855"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19410..19412),aa:Val,seq:tac)
     gene            complement(19481..19556)
                     /locus_tag="ID725_RS14280"
                     /old_locus_tag="pgaptmp_002856"
     tRNA            complement(19481..19556)
                     /locus_tag="ID725_RS14280"
                     /old_locus_tag="pgaptmp_002856"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19521..19523),aa:Lys,seq:ttt)
     gene            complement(19721..20512)
                     /gene="cpoB"
                     /locus_tag="ID725_RS14285"
                     /old_locus_tag="pgaptmp_002857"
     CDS             complement(19721..20512)
                     /gene="cpoB"
                     /locus_tag="ID725_RS14285"
                     /old_locus_tag="pgaptmp_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGV7851405.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20522..21043)
                     /gene="pal"
                     /locus_tag="ID725_RS14290"
                     /old_locus_tag="pgaptmp_002858"
     CDS             complement(20522..21043)
                     /gene="pal"
                     /locus_tag="ID725_RS14290"
                     /old_locus_tag="pgaptmp_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGV7851406.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21078..22370)
                     /gene="tolB"
                     /locus_tag="ID725_RS14295"
                     /old_locus_tag="pgaptmp_002859"
     CDS             complement(21078..22370)
                     /gene="tolB"
                     /locus_tag="ID725_RS14295"
                     /old_locus_tag="pgaptmp_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGV7851407.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22503..23777)
                     /gene="tolA"
                     /locus_tag="ID725_RS14300"
                     /old_locus_tag="pgaptmp_002860"
     CDS             complement(22503..23777)
                     /gene="tolA"
                     /locus_tag="ID725_RS14300"
                     /old_locus_tag="pgaptmp_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGV7851408.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKKAAEKAAAEKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADK
                     KAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNG
                     ASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQ
                     AALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(23842..24270)
                     /gene="tolR"
                     /locus_tag="ID725_RS14305"
                     /old_locus_tag="pgaptmp_002861"
     CDS             complement(23842..24270)
                     /gene="tolR"
                     /locus_tag="ID725_RS14305"
                     /old_locus_tag="pgaptmp_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGV7851409.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24274..24966)
                     /gene="tolQ"
                     /locus_tag="ID725_RS14310"
                     /old_locus_tag="pgaptmp_002862"
     CDS             complement(24274..24966)
                     /gene="tolQ"
                     /locus_tag="ID725_RS14310"
                     /old_locus_tag="pgaptmp_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGV7851410.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(24963..25367)
                     /gene="ybgC"
                     /locus_tag="ID725_RS14315"
                     /old_locus_tag="pgaptmp_002863"
     CDS             complement(24963..25367)
                     /gene="ybgC"
                     /locus_tag="ID725_RS14315"
                     /old_locus_tag="pgaptmp_002863"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGV7851411.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25517..25810)
                     /gene="ybgE"
                     /locus_tag="ID725_RS14320"
                     /old_locus_tag="pgaptmp_002864"
     CDS             complement(25517..25810)
                     /gene="ybgE"
                     /locus_tag="ID725_RS14320"
                     /old_locus_tag="pgaptmp_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGV7851412.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(25810..25923)
                     /gene="cydX"
                     /locus_tag="ID725_RS14325"
                     /old_locus_tag="pgaptmp_002865"
     CDS             complement(25810..25923)
                     /gene="cydX"
                     /locus_tag="ID725_RS14325"
                     /old_locus_tag="pgaptmp_002865"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGV7851413.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(25938..27077)
                     /gene="cydB"
                     /locus_tag="ID725_RS14330"
                     /old_locus_tag="pgaptmp_002866"
     CDS             complement(25938..27077)
                     /gene="cydB"
                     /locus_tag="ID725_RS14330"
                     /old_locus_tag="pgaptmp_002866"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGV7851414.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27093..28661)
                     /gene="cydA"
                     /locus_tag="ID725_RS14335"
                     /old_locus_tag="pgaptmp_002867"
     CDS             complement(27093..28661)
                     /gene="cydA"
                     /locus_tag="ID725_RS14335"
                     /old_locus_tag="pgaptmp_002867"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGV7851415.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7173 a         7630 c         7096 g         6902 t
ORIGIN      
        1 tttcattaat ttaagttggc taaatgcagt taaaattttt aacggccagc cacccaaaat
       61 cgtcgcttcc atttcccgga gtaataatga agcatgaatt atcaagtatg aaggcatttg
      121 tcatactggc agagtccagt tcatttaaca atgccgctaa attactcaat attacgcaac
      181 ctgcattaac acgaagaata aaaaagatgg aggaagattt acatattcaa ctatttgagc
      241 gtacaactcg caaggttacg ttaacaaaag caggaaaaag gttgctccca gaggcgcggg
      301 aattaataaa gaaattcgat gaaacgcttt ttaatattcg tgatatgaat gcttatcatc
      361 gtggtatggt gacgttagcg tgtattccga ccgcagtatt ttatttttta ccgctggcaa
      421 tcggtaaatt taatgagctg tatcccaata ttaaagtgcg gattctggaa caaggcacga
      481 ataattgcat ggaatcggtg ctgtgtaacg aatcagactt tggcataaac atgaacaacg
      541 tcacaaattc atccattgat tttaccccac tggtcaacga gccgttcgtg ctggcctgtc
      601 ggcgtgacca tccattggct aaaaagcagc ttgtagaatg gcaggagtta gtgggttaca
      661 aaatgattgg cgtacgttcc tccagtggca accgattgtt gatagaacaa cagctagccg
      721 acaagccctg gaagctggac tggttttacg aagtgcgtca tctttcgacg tcattaggac
      781 tggtagaggc gggactggga atttcggcgc ttcctggtct ggcaatgccc catgcgccct
      841 attcttctat tattggtatc ccactggtgg aacccgttat acgtcggaca ttggggatta
      901 ttcgccgcaa agatgccgtg ctttctccgg cagcagaacg cttttttgcc ttgctgatta
      961 atttgtggac tgacgataaa gacaatttgt ggaccaatat cgtcgaacgc cagagacatg
     1021 cgttacagga gataggttaa tacaccggcg caggagagac tgcgccgggt aaatcagcgg
     1081 ttagtgtgcg ttaaccacca cccacattgg tccctgcccg accgcgtagc ggcctttttc
     1141 atgcagtagc ccctgctcac caacaatttc gtataccgag atgtggtgcg atttttgccc
     1201 ggcggcaatc agatacttac cacggtgatc gatattaaag ccgcgcggct gggtttcagt
     1261 tggctggaag ccctctttac tcaacacgct gccatcttcc gaaacgctga agacagtaat
     1321 caggctggcg gtacggtcac aggcatataa gtgacgacca tccggggtaa tatgaatatc
     1381 agccgcccaa cgggtgtcgg agaagttttc cggcatcata tccagcgtct ggacacattc
     1441 gatattgccg tgcggatctt tcagttccca gacatccact gagctgttta actcattgac
     1501 gcaatatgca tattgttcgt ttggatggaa taccatatga cgcgggccgg ccccttcaac
     1561 ggtagtcact tccgcagggt cctgcgccac cagatgacca tcatcgctga ccgtaaacag
     1621 gcaaatgcga tcctgcttta atgccggaac ccacagcgta cggttgtccg gtgagatatt
     1681 ggcggaatgg caaccgtcca gcccctcgac cacatcgacg acgccaactg gcaggccatc
     1741 ttccagacgc gttacgctca cgttacccgc attgtaagaa cctacaaaga caaactgccc
     1801 ctggtgatcg gtggaaatat gcgtcggact acccggcagc gcagactctg cggcaaaggt
     1861 cagtgcgcca tcgtccgggg cgatacgata cgccaggacg cgaaactcag ggcgaacacc
     1921 aacatagaga taacgtttgt ccgggctgac caccatcggc tgcacctgcc ccggcacatc
     1981 gacaacctgt gtcagcgtca gtgcgccttc atgattcagg ttccagacgt gaatttgctg
     2041 gctctcaggg ctggcgatat aaactgtttg cttcatgaat gctcctttgc atttagcaac
     2101 tgactgtaac agctaaaatt agtcgctttt ggcggtgaat gctgaattaa aacgcagaat
     2161 tttgtccgat agtggtatcg gtgtaccatt cgcgcagaag aaaattctta accttaatct
     2221 ggaatacgcc atgaccacac gcgtgattgc tctcgactta gacggcacct tattgacccc
     2281 gaaaaagacc ctgctacctt catcgataga agccctggcc cgcgctcgcg aagcaggtta
     2341 tcaattaatc atcgtcacag gtcgccatca cgtcgctatt catccttttt atcaggcgct
     2401 ggcgctggat acacctgcta tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa
     2461 aaccgtgctg gaagcggacc caatgcccgt taataaagcc ctacaactca ttgagatgct
     2521 gaatgaacac cacattcacg gtctgatgta tgtcgatgac gagatggttt atgagcaccc
     2581 gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc
     2641 gactttcaca caagtcgctt ctctggctga aacggcgcaa caagttaacg ccgtatggaa
     2701 gttcgccctc acgcacgatg acctgccgca attgcagcat tttggtaagc atgtcgaaca
     2761 tgaactggga ctggagtgtg aatggtcctg gcacgatcag gttgatattg cacgcggcgg
     2821 caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt cgatggaaaa
     2881 cgtcgtggcg ttcggcgata actttaatga tatcagtatg ctggaggccg ctggtacagg
     2941 cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg tgattggtga
     3001 taacaccacc gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta
     3061 tcgacacact tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc
     3121 tggcccacgg gctgatacgc gcccacagcg ttttaccgcc gacttccagt tccacttcca
     3181 cctggccgtt gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg
     3241 tttgctgcgg cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg
     3301 cagcttgcag cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg
     3361 cggtcatcgc gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt
     3421 gctctttcgg cagccacgga ttcatcacgc tactacccca gacttcttcc agcgcgccaa
     3481 aggctttcac ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat
     3541 ccagcgaatg gctgacatac aacatcggga tgttgatttc ccgcgtcaga cgttgcagat
     3601 aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca
     3661 gcaattctgg tgccgtcagc aaagcccgcc caatcgctac gcgctgtttt tcgcctccgg
     3721 acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat
     3781 cgaactgatc gaccatgctt ttcgccatgc cgtagcgcag attgccacgc actttgtaat
     3841 gcgggaacag ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc
     3901 agataccttt ttcggcatca tttagtaccc gaccattgag gacaatcctc cctttttgcg
     3961 ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa
     4021 agatagcagt gatgccattg gcgggcagcg tttcattaat agtcaggcaa tggttgccca
     4081 acgtctggga aaaattcagt tccagcatga ttagcgcccc gcccgttcac ggctgattct
     4141 ggccagccat tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag
     4201 tctcgctgcg ccactttccc cgccgggggt ctgaatcagg gtatacatgg cagaaggaat
     4261 ggttcgcgtt tcaccaggaa tgttcgacac aaaggtgatg gttgcgccaa attcaccgag
     4321 agaacgagca aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt
     4381 gatagtaaag aaaacgcgcc agcgcccggc ccccagtgtt cttgcggcct gttccagttt
     4441 gacatcaacc ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac
     4501 ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata
     4561 cagacgttca ccgataaatc cgcgccgccc catcgaaact aataataagt aaccgacgac
     4621 cacgggcggt aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa
     4681 cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc
     4741 cagggaagaa actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat
     4801 tacttggttg taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa
     4861 tcataaaatg ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc
     4921 acttttttat gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctagcaact
     4981 gcgtcagaac cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg
     5041 ccacgaacat cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt
     5101 tgcagtgctt cttttgcata aatgccagcg ggaacatgtt ccggatcgcc aaccgccagg
     5161 cgaccgccat tcagcagtga agtccagttg gttttgctgt cgatggtaaa atctttctgc
     5221 tcgctggctt tcggtgctac aacgaccagg ctattgccga gcagtgtctg acgcgtagct
     5281 gtatcgatcg cttttttatc aaccgcataa tccatccatt tctgatcggc agaaataaac
     5341 agatccgcag gggcacccgc ttcaatctga cgggcgagag tagatgacga agcgaaagaa
     5401 gaaaccacat ccacgccttt ctctttttta tactgcgtag caatgtcctg cattgcgtta
     5461 gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat ctgccagtgc attgccagca
     5521 acagcgaaag agagtgccgc cccagcaaac aagttcaacc atttacgagc cattggtaac
     5581 cccttaatgt aacgttatgt aggcgacaat ataacgacta actcggaatt ttcccagcag
     5641 ttattgctaa cctttgtgag gtagataaga aaaaatatcg gcaggaaaag caggaagttg
     5701 agaaaaagaa aatgcccgac taagcgggca ttcagggaat caatgatttt gtccgggctg
     5761 gtctttttta ccaacaccag aaaagatgtt gaatacttca ccaagaccgt aaatcagacc
     5821 caggatgatg gccatcacga caggtaccat gattacggcg aataccagac tttttaataa
     5881 ctctaacatg gtcaactcca gatatagtca tgagactatt ctaaccgcta agcacagaaa
     5941 agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg ttttccgtca caataagact
     6001 tttgccagga cattgttatg caggccgaaa tccttctcac ccttaagctc caacaaaaat
     6061 tattcgccga cccgcgccgc atttcgctac taaaacacat tgcgctttcc ggttccatta
     6121 gccagggagc gaaagatgcc ggtatcagct ataaaagcgc ctgggatgcc attaacgaga
     6181 tgaatcagtt aagtgagcat attctggtcg agcgcgcaac aggcggtaaa ggtggcggcg
     6241 gcgcagtact gacccgctat ggtcagcgac tgattcagct ctatgactta ctggcgcaaa
     6301 tccagcaaaa agcctttgat gtgttaagtg acgatgacgc cctgccgctg aacagcctgc
     6361 tggccgcgat ctcacgtttt tcactccaaa ccagcgcccg taaccagtgg ttcggtacca
     6421 tcaccgcccg cgatcatgat gacgttcaac agcatgttga tgtcttactg gctgatggaa
     6481 aaacacgcct gaaagtcgca attaccgcac aaagcggcgc gcgtctgggg ctggatgaag
     6541 gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg cattactcag gacgaagcgg
     6601 tcgcgcaaaa cgctgacaac caattaccgg gtattattag tcatattgag cgcggcgcag
     6661 agcagtgcga agtattaatg gcgctaccgg acgggcaaac actgtgcgcc acagtgccgg
     6721 taaatgaagc gacttctctt cagcaaggac agaatgtcac ggcctacttt aatgccgaca
     6781 gcgtgattat cgccacactg tgctaagcgt gttgacaatt tgttatgaaa cacgtatccc
     6841 tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat cgttgcaaat tttgcaaggc
     6901 acgtttcgtc ttagcgacac aaaaacgctg caattgcctc agctaacgtt aaacgcgggt
     6961 gatagttggg cgtttgtcgg ttcgaatgga agcgggaaat cggccctggc ccgcgcgctg
     7021 gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc agttttccca catcactcgt
     7081 ctctccttcg agcaattgca aaagctcgtc agcgacgaat ggcagcggaa taacaccgat
     7141 atgctcggcc ctggcgaaga tgacaccgga cgcactacgg ctgagattat tcaggatgaa
     7201 gtaaaggatg caccgcgctg catgcaactg gcacagcagt tcggtattac ccccctcctc
     7261 gaccgacgct ttaaatacct ttccactggc gagacgcgaa aaaccctgct gtgtcaggcg
     7321 ctgatgtcgg agcctgactt gttgattctt gatgagccgt tcgatggcct ggatgttgcc
     7381 tcacgtcagc agctggctga gcgactcgcc tcgttacatc agtccggtat tactctggta
     7441 ctggtgctca atcgcttcga tgagatcccg gagtttgtcc agtttgctgg cgtgctggcg
     7501 gattgcacgt tagcggaaac tggcgctaaa gaggaactgc tccaacaagc actcgtcgcg
     7561 caactggcgc atagtgaaca gcttgaaggt gtgcaactgc cggagccgga tgaaccttca
     7621 gcacgtcacg ccttacccgc caacgaaccg cgcattgtgc tgaataatgg cgtggtttct
     7681 tataacgatc gccccattct taataacctt agctggcagg tgaatccagg tgaacactgg
     7741 caaattgtcg ggccaaatgg cgcgggaaaa tcgacgttat taagcctgat tactggcgat
     7801 catccgcaag gttacagcaa cgatttgacg cttttcggac gacgtcgtgg cagcggcgaa
     7861 accatctggg atatcaaaaa gcatatcggt tacgtcagca gtagtttgca tctggattac
     7921 cgggtcagta ctactgtgcg taatgtgatt ctttctggct attttgattc gattggcatt
     7981 tatcaggccg tttcggatcg ccagcaaaaa ctggtgcagc agtggctgga tattctcggc
     8041 attgataaac gcacggctga cgctccgttc catagtcttt cctggggaca gcagcgtctg
     8101 gcgctgatcg tccgcgcact ggtgaaacat ccgacgttgc ttattctcga cgaaccattg
     8161 caggggcttg atccgctcaa tcgccagctt attcgccgtt ttgttgatgt gctgattagc
     8221 gaaggtgaaa cgcaattgtt gtttgtttcg caccatgctg aagatgcgcc ggcttgtatt
     8281 acccatcgtc tggagttcgt gccggacggt ggcctctatc attatgcgct gacaaaaata
     8341 aactaagtcg gtaatgctga ctgtgccgga tgcggcgtaa acgctttatc cggcacacgg
     8401 tcagacgcat ccaggcatga aactgcgtct ttttttcaga taaaaagcgc aatcattcac
     8461 aatccctctg ttttataatc acttaatcgc gcataaaaaa cggctaaatt cttgtgtaaa
     8521 cgattccact aatttattcc atgtcacact tttcgcatct ttgttatgct atggttattt
     8581 cataccataa gcctaatgga gcgaattatg agagttctgg tcactggtgg tagcggttac
     8641 attggaagtc atacctgtgt gcaattactg caaaacggtc atgatgtcat cattcttgat
     8701 aacctctgta acagtaagcg cagcgtactg cctgttatcg agcgtttagg cggcaaacat
     8761 ccgacgtttg ttgaaggcga tatccgtaac gaagcgttga tgaccgagat cctgcacgat
     8821 cacgctatcg acaccgtgat ccacttcgcc gggctgaaag ccgttggcga atcggtacaa
     8881 aaaccgctgg aatattacga caacaatgtc aacggtactc tgcgcctgat tagcgccatg
     8941 cgcgccgcta acgtcaaaaa ctttattttt agctcctccg ccaccgttta tggcgatcag
     9001 cccaaaattc catacgttga aagcttcccg accggcacac cgcaaagccc ttacggcaaa
     9061 agcaagctga tggtggaaca gatcctcacc gatctgcaaa aagcccagcc ggactggagc
     9121 attgccctgc tgcgctactt caacccggtt ggcgcacatc cgtcgggcga tatgggcgaa
     9181 gatccgcaag gcattccgaa taaccttatg ccatacatcg cccaggttgc tgtaggccgt
     9241 cgcgactcgc tggcgatttt tggtaacgat tatccgaccg aagacggtac tggcgtacgc
     9301 gattacatcc acgtaatgga tctggcggac ggtcacgtcg tggcgatgga aaaactggcg
     9361 aacaagccag gcgtacacat ctacaacctc ggtgctggcg taggcagcag cgtgctggac
     9421 gtggttaatg ccttcagcag agcctgcggc aaaccggtta actatcattt tgcaccgcgt
     9481 cgcgagggcg accttccggc ctactgggcg gacgccagca aagccgaccg tgaactgaac
     9541 tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca cctggcactg gcagtcacgc
     9601 catccacagg gatatcccga ttaaggaacg accatgacgc aatttaatcc cgttgatcat
     9661 ccacatcgcc gctacaacca gctcaccggg caatggattc tggtttcacc gcaccgcgct
     9721 aagcgcccct ggcagggggc gcaggaaacg ccagccaaac aggtgttacc tgcgcacgat
     9781 ccagattgct tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa ccccgattac
     9841 accgggactt acgttttcac taatgacttt gcggccttga tgtctgacac gccagatgcg
     9901 ccagaaagca acgatccttt gatgcgttgc cagagcgcgc gcggtaccag ccgggtgatc
     9961 tgcttttcac cggatcacag taaaacgctg ccagaactga gcgttgcggc attgacggaa
    10021 atcgtcaaaa cctggcagga gcaaaccgca gaactgggga aaacataccc gtgggtgcag
    10081 gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc cgcatccgca cggacagatt
    10141 tgggcaaata gcttcctgcc taacgaagct gagcgcgaag accgcctgca aaaagaatat
    10201 tttgccgagc agaaatcgcc aatgctggtg gattatgttc agcgcgagct ggcagacggt
    10261 agccgtaccg ttgtcgaaac cgaacactgg ttagcagttg tgccttactg ggctgcctgg
    10321 ccgttcgaaa cgctacttct gcccaaagcc cacgttttgc ggatcaccga tttgaccgac
    10381 gcccagcgca gcgatttggc actggcgttg aaaaagctga ccagtcgtta tgacaacctc
    10441 ttccagtgct ccttccccta ctctatgggc tggcacggcg caccgtttaa tggcgaagag
    10501 aatcaacact ggcagctgca cgcgcacttt tatccgcctc tgttgcgctc cgccaccgta
    10561 cgtaaattta tggttggtta tgaaatgctg gcagaaaccc agcgagacct gaccgcagaa
    10621 caggcagcag agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc cggagtgtaa
    10681 gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg ctaccctgcc
    10741 actcatacca ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac cgactacaac
    10801 gacggtttcg ttctgccctg cgcgattgat tatcaaaccg tgatcagctg tgcgccacgc
    10861 gatgaccgta aagttcgcgt aatggcagcc gattatgaaa atcagcttga tgagttttcc
    10921 cttgatgcgc ccattgtcgc gcatgaaaac tatcaatggg cgaactacgt tcgtggcgtg
    10981 gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg tggacatggt gatcagcggc
    11041 aatgtgccgc agggtgccgg gttaagttct tccgcttcac tggaagtcgc ggtcggaacc
    11101 gtattgcagc agctttatca tctgccgctg gacggcgcac aaatcgcgct taacggtcag
    11161 gaagcagaaa accagtttgt tggctgtaac tgcgggatca tggatcagct aatttccgca
    11221 ctcggcaaga aagatcatgc cttgctgatt gactgtcgct cactggggac caaagcagtt
    11281 tccatgccga aaggtgtggc tgtcgtcatc atcaacagta acttcaaacg taccctggtt
    11341 ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg gtgcgcgttt cttccagcag
    11401 ccagctctgc gcgatgtcac cattgaagag ttcaacgctg ttgcacatga gctggaccca
    11461 atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg cccgcaccgt tgaagctgcc
    11521 agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt tgatggcgga gtctcatgcc
    11581 tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg acactctggt agaaatcgtc
    11641 aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt tggcggctgt
    11701 atcgtcgcgc tgatcccgga agagctggtg cctgccgtac agcaagctgt cgcagaacaa
    11761 tatgaagcaa aaacaggtat taaagagact ttttacgttt gtaaaccatc acaaggagca
    11821 ggacagtgct gaacgaaact cccgcactgg cacccgatgg tcagccgtac cgactgttaa
    11881 ctttgcgtaa caacgcaggg atggtagtca cgctgatgga ctggggtgcg actttacttt
    11941 cagcccgtat tccgctttcc gatggcagcg tccgcgaggc gctgctcggc tgtgccagcc
    12001 cggaatgcta tcaggatcag gccgcgtttc tgggtgcctc tattggtcgt tatgccaacc
    12061 gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt gacgctttcg ccaagtcagg
    12121 gcgttaacca gctgcacggc ggcccggaag ggttcgacaa acgtcgctgg cagattgtga
    12181 accagaacga tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat cagggcttcc
    12241 cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga cgataaccgt atctccatta
    12301 cttatcgcgc cacagttgat aaaccttgcc cggtgaatat gactaatcac gtctatttca
    12361 atcttgacgg caagcagtct gacgtgcgca atcacaagtt gcagattctg gcggacgaat
    12421 atctgccggt tgatgaaggc ggtattccgc acgacggcct gaaatctgtc gccggaacgt
    12481 cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt tcttgccgac gacgatcagc
    12541 gcaaagtgaa aggttacgat cacgcattct tgttacaggc caaaggcgat ggcaagaaag
    12601 tggcggcgca tgtctggtca gcagatgaaa aattgcagct gaaggtctac accaccgctc
    12661 cggctctgca attctactcc ggcaactacc tcggcggcac accgtcgcgg ggaaccgaac
    12721 cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt tctgccggac agcccgaacc
    12781 accctgaatg gccgcaaccg gactgtttcc tgcatcctgg tgaagagtat tccagcctga
    12841 cggaatatca gtttattgct gagtaatgtt aagccctcgt catgagggct ttatctcata
    12901 ttgttcaaat caccagcaaa caccgacata tttgcaactc aatattcaca acaaccttac
    12961 actgcgccac tattttcgct atggttatgc gtaagcattg ctgttgcttc gtcacggcaa
    13021 tataatgaga attattatca ttaaaagatg atttgaggag taagtatatg gctgtaacta
    13081 agctggttct ggttcgtcat ggcgaaagtc agtggaacaa agaaaaccgt ttcaccggtt
    13141 ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc aaaagcagca ggtaagctgc
    13201 tgaaagagga aggttacagc tttgactttg cttacacctc tgtgctgaaa cgcgctatcc
    13261 ataccctgtg gaatgtgctg gacgaactgg atcaggcatg gctgcccgtt gagaaatcct
    13321 ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct gaacaaagcg gaaactgctg
    13381 aaaaatatgg cgacgagcag gtgaaacagt ggcgtcgtgg ttttgcagtg actccgccgg
    13441 aactgactaa agatgatgag cgttatccgg gtcacgatcc gcgttacgcg aaactgagcg
    13501 agaaagaact gccgctgacg gaaagcctgg cgctgaccat tgaccgcgtg atcccttact
    13561 ggaatgaaac cattctgccg cgtatgaaga gcggtgagcg cgtaatcatc gctgcacacg
    13621 gtaactcgtt acgtgcgctg gtgaaatatc tcgataacat gagcgaagaa gagattcttg
    13681 agctgaatat cccgaccggc gtgccgctgg tttatgagtt tgacgagaac ttcaaaccgc
    13741 tgaaacgcta ttatctgggt aatgctgacg agatcgcggc gaaagcagca gccgttgcga
    13801 accagggtaa agcgaagtaa acgtcattcg tttaaaaatg agaaagccga ctgcaagtga
    13861 gtcggctttt ttgtttgtcg tcgctaacaa tggagcacat tgcctgatgc gacgctgcgc
    13921 gtcttgtcag ggctacggtg attcatcgga tacgccactc tgacggcgca tccgacaatt
    13981 aaactttacc cgcgacgcgc tttcactgcg ttcgccagtt gacgtaacag agcatcggta
    14041 tcttcccagc caatgcaggc atcggtgatg ctcttaccat aggccagcgg ctccccgctc
    14101 tccaggctct gattgccttc caccagatga ctttccacca tcacaccgat aatggccttt
    14161 tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt tttgaattgt
    14221 ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc tgctttgttc
    14281 agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt accgccgcgc
    14341 agaatgatat ggcaatcgcc gttaccgctg gtattcacaa tcgccgaatg cccccattta
    14401 gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat agccacttta
    14461 atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga cgccagttcg
    14521 cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat caggtcagcg
    14581 agatactgag gagtgatcat atcgaggaat tcacccgccg ctggcagacc gctgtcgtta
    14641 atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa gctgttatcc
    14701 atatgcggat cgttaatcag ccctttccag cccaccgtag tacgcggctt ttcaaaatag
    14761 acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag caagcgagtg
    14821 gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac caccaacagg
    14881 cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc aaccgtattc
    14941 gcggcatttt cagtagcggg gaatttttcc agcaatgcga caggaggaag taactctttg
    15001 atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt gttgccatac
    15061 ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg taaacggggt
    15121 tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa
    15181 aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt atttcaagat
    15241 tctctgctgg atttctttgc ctggctgaaa tcttaaacca atgccttata ttcacctgca
    15301 aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat cctatgaaaa
    15361 tgacaaaact ggccacacta tttctgactg ccactctaag ccttgccagc ggtgccgcac
    15421 tggctgccga tagcggagcg caaaccaata acggccaggc aaacgccgca gctgatgcgg
    15481 gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg
    15541 taaataccgg ttctggcggc actatgctgc atccggatgg ttcttcaatg aacaatgacg
    15601 gcatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcgc tgcccggata
    15661 tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc aaaaccgacg
    15721 gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct ctccctttta
    15781 atttttccct tctaaaagca gtatgatgtc ataaagttaa gtgagaataa ttaagggatg
    15841 actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag ataataatgc
    15901 tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcattgg
    15961 tggttttctt tccggttctc tggctttgct ggccgatgcg ggtcatatgt tgaccgatac
    16021 tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg
    16081 ccacactttc ggttggctaa gattgaccac gctcgcggcg tttgtgaatg ccatcgccct
    16141 cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc
    16201 ggtcgagggc ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc
    16261 tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact
    16321 gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat
    16381 ctggaccggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct
    16441 gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgagg gtgcaccggt
    16501 atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa
    16561 tgtgcaccat gtgcatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca
    16621 ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga
    16681 tcactatcag attgagcacg ccaccattca gatggaatat caaccttgtc atgggccaga
    16741 ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat gggacagcgc
    16801 gcgtgagcct ctttcgcgtg cgctattgat ccacatccgg ctgccgttga gcgcaataaa
    16861 ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac
    16921 gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac gagtcatcag
    16981 aatcattgcc acgatagata acaccatcat gcaagaatcc cagaacggga aagcgtccgg
    17041 ttgtagttca ggcatcgcaa cctgtaatcc taatgcttgc atgatgatga ctgccacgcg
    17101 ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca
    17161 aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttta actccgcctc
    17221 gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa
    17281 cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt
    17341 gacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca
    17401 cagcaacccg gcgattgtgc ctaccgcttc gatccatgag agatcataac cgcctgctcc
    17461 tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg
    17521 taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc agcatacgat
    17581 tgagcggcac cagcgccctc tctcgcagcc tttcatcaac atgaacctcg taattgcttc
    17641 cttcctgttc taatgcctca gcgatggcct ggaggccatt catggccatc cacggacaat
    17701 gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat
    17761 ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca ataagcctct
    17821 gatgtggcaa tgttttcgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca
    17881 tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt
    17941 cttgcaagcg ggttaacgcc tgagtcttaa actcatcatg cacaatgcag gcaccctgcc
    18001 agcataaaat gtctgcgccc gtcttttttt gcacgtaacg ccccagatgt ttgtcgggtg
    18061 cccagatgat tttttcaccc aaactatcca gatgatcaat aagttcgacg gcaatgcttg
    18121 aagttaccac ccaatctgcc cgcgctttta ccgcagcaga agtgttggca tagacgacga
    18181 cagtacgatc gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat
    18241 ccagtgaaca ctcagcctga agtgtcggca tcagaattgt tttttccgga ctgagaattt
    18301 tggcggtttc tcccataaat ctcaccccag cgactaacaa cgtagaagcg ggatgctttg
    18361 caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct tctgccagtt
    18421 gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca
    18481 gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg
    18541 gggggaaagg ataaatcgcc gtgtctggat caaacatcac gctcatctta ccatctcgtt
    18601 ttacaggctt aacgttaaaa ccgacattag cgtgatattc gctgttatcg gtgtaatttg
    18661 ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgtatgag
    18721 atgccgaatg ccacactttg cgtcttattc ctccgacgta aagttgttta cgcagcaagc
    18781 gcggagtatg tcatcagggt caagaattga aattgagaga tgcctgaata gcaaaaaagc
    18841 gcctttaggg cgctttttta cattggtggg tcgtgcagga ttcgaacctg cgaccaattg
    18901 attaaaagtc aactgctcta ccaactgagc taacgaccca tacgggtgtt acctgaaaga
    18961 tttctactcg gaaccaatct ttaaaattgg tgggtcgtgc aggatgactc gcttcgcttg
    19021 ccctacgggc cgttgctgac gcaacgttat ccttcacgct taacatctga gtttgatgtt
    19081 aaaattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
    19141 taccaactga gctaacgacc cgagtggtgg gtgatgacgg gatcgaaccg ccgaccccct
    19201 ccttgtaagg gaggtgctct cccagctgag ctaatcaccc gatactgcgc tggatactac
    19261 aaactacttc caccgaaccc accgtaatta gtggtgggtc gtgcaggatt cgaacctgcg
    19321 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccggt ggtgggtgat
    19381 gacgggatcg aaccgccgac cccctccttg taagggaggt gctctcccag ctgagctaat
    19441 cacccgatat tacgctggat actacaacta actaggttag tggtgggtcg tgcaggattc
    19501 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt
    19561 tttcgttgct ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc
    19621 agacttgagc gcaacaaaaa tttcgatgta gatcactcaa ctgcttatga ttcgcacgac
    19681 acgaccagaa ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt
    19741 tttgtgcctg tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg
    19801 ctttcgcttt tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat
    19861 ctgcagcctt tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat
    19921 catctttttt acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt
    19981 aagttgaatc agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc
    20041 gggatttatc ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcaccgcttt
    20101 tcaccggcgc gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgccg
    20161 caccgctttg atcaccgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga
    20221 tctgcaacag gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct
    20281 gaatctgacc acgcagggaa tcaatatcgg attgattatc agagagttgt tgctggagtt
    20341 gggttaaaag ctggctgtga gcattagaaa tacgctcaag ttgaatgacg cggtcttcga
    20401 ccgagcctga gccgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta
    20461 taccaaccag taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct
    20521 cttagtaaac cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta
    20581 ctgcaggttt ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac
    20641 cctgcaggta catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg
    20701 gagtaccacg ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca
    20761 ggaagtttgc atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca
    20821 gatcgaagta aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt
    20881 cggaagacat gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc
    20941 cttcgctgcc gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag
    21001 gcagagcaat catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac
    21061 tattcaatta attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca
    21121 gttgccggaa gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc
    21181 atcccctgag aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca
    21241 tccaggaacg tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg
    21301 tgctgctgcc caccattgga gctgaccatt accataaatt taccgtcgct gctgacatcc
    21361 gcatcctggt tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc
    21421 actttataaa cctgtggacg accggcctgg tcagaagtaa atgccaggtt ctggctgtcc
    21481 gggaaccagg tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca
    21541 gaagccaaat ccattacgta caggttcagg ctaccggttt tcgacaaggc gaatgccagt
    21601 ttgctgccgt ctggcgagaa tgcaggcgca ccgttgtgac gcgggaatga agccacctga
    21661 cgtacagcgc cgtttgccag cgtttgtata accagcgcgg aacgaccgct ttcgaaggtc
    21721 acataagcca gtttagaacc gtctggtgac cacgccgggg acatcagcgg ctgtggtgaa
    21781 cggtgaacga caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac
    21841 tgaccgccgt tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaataccg
    21901 gtcagttttt caaacacttc atcactggcg gtatgaccag cataacgcag ccactgcttg
    21961 ttcactttgt acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt
    22021 tgataagcaa cattgtaaga accatccgga ttcggagtga cctgaccgac aactacagcg
    22081 tcaataccca gcgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt
    22141 ggcagacgag cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc ggcagcaacg
    22201 atgccgccaa tatcttcagg tgccgcacca gggcccgccc actggaaagg aacaactcca
    22261 ataggacgac cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact
    22321 gatgcccaca gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc
    22381 ttatctggac cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa
    22441 actaccagaa ccccgtggca accggtgcct gatgttgacc gtcggaacag tcaacatcgc
    22501 gattacggtt tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt
    22561 ggtttcggga tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg
    22621 ccaccttcag gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta
    22681 caggttttgc ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc
    22741 tgcccggcat agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc
    22801 ccggcaggcg aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca
    22861 gagcttagct caccgaaaat atcatctgcc tccgcggcag cttttgctgc agcggctttt
    22921 tcagctgctg cttttgcagc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct
    22981 ttcttatcag ctgctgcctt ttcagcagcc gctttttctg ctgctttttt gtcggctgca
    23041 gctttctctg ctgctgcctt ttcagcagcc gctttttctg ctgctttttt gtcggctgca
    23101 gctttctctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt ctttcttgct
    23161 tcagctgcag ctgcttctgc cgcttccgct ttcttcttca gcgccgcagc tgctgcctcg
    23221 gcttttttct gcgcttcggc tgcggctttg gcggcttctg cttctgcttt tttcttcgcg
    23281 tctgcagccg ctttcttcgc tgcttcttcc gcagctttat catccgcttc ggccttcgct
    23341 ttagcatctg ccgccgcttt cgctgccgcc tcttccgctt gcttctgctt taactcggcc
    23401 tgttttgcgg cttcttcagc ctgctttttc tgttcctgag cagctaaccg ctctttctca
    23461 agttgcttca ggcgttcctg ttcagccgct tgtttctcac gcagttcttc agcagcctgc
    23521 tgttccttca tcttgcgctg ctcatcagaa cgcttcgcgc ttgattcctg gctttgcatg
    23581 cgtttgtact gctcaactac cgcacctgaa tcaaccatga cagcgtcgat ggacgaacca
    23641 ccgccgcctc cagctgaagc ttctatattc tcatcgaacg aactccagat cagcgccgca
    23701 aataagatga catgcagcac tgctgaaata attatcgccc gcttgagctt gtcgttttgt
    23761 tcggttgcct ttgacactct cggtttccaa aaactgttcg cctgttaccc gctctctttc
    23821 aagcaaggga aacgcagatg tttagatagg ctgcgtcatt aaaccaaccg atttcacacc
    23881 cgcactatgt aacaagttca gtgctttaat tatttcatcg taaggcacat cttttgcgcc
    23941 accgatcaga aagaccgttt tcgggttggc cttgaaacgg ctggacactt ccgccaccac
    24001 ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc accacggtgt actgaccaat
    24061 accagacact tcaacaatca ctggcggatt atcgttactg ctcaccgcct gtgattcagt
    24121 agcgtctggc aaatcgacct ccacgctctg ggtgatgatg ggcgctgtcg ccataaagat
    24181 cagcaacagc accagcagta cgtccagcaa cggtacaatg ttgatttcgg acttgagatc
    24241 gcgacgacct cgtccacgcg ctctggccat ggcttacccc ttgttgctct cgctaacggt
    24301 aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata aagttgtcgt aattcagttc
    24361 cagtttgttt acacgttggt tgagacggtt ataagccatt accgccggaa tagcggcgaa
    24421 cagaccaatt gccgtcgcaa tcaacgcttc tgcgataccg ggcgcaacca tttgcagtgt
    24481 tgcttgtttt accgccccga gggcgataaa ggcgtgcatg atcccccaga ccgtaccaaa
    24541 cagaccaata tacgggctga tggagccaac cgtaccgagg aacggaatgt gcgtttccag
    24601 attttcaagt tcgcggttca tggaaatacg catcgcacgc gacgcccctt ccactacggc
    24661 ttccggcgca tggctattgg cacgatgcag gcgcacaaat tctttgaacc cgctgtaaaa
    24721 gatttgttcc gaaccagtca gattatcccg tttcccctgg ctctcttgat agaggcgaga
    24781 gagttcgatt ccagaccaga atttatcttc aaacgcttcg gcttcgcgcg ccgctgcgtt
    24841 aagaatacgg gtccgctgga taataatggc ccaggatgcg attgaaaaac caatcaaaat
    24901 caacatgata agtttaacca gaaggctagc cttcaggaac aaatcaagga tattcatgtc
    24961 agtcactgct taaactccgc gacaatagac ttgggaagcg cacgaggctt cattttgagt
    25021 gggtcaacgc aaacaaccag aacctctgct tcattcagca aagtattctc ggcgttgaca
    25081 atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg ttatttcagt ctgtatttcg
    25141 agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg tcattttacg taccacaaag
    25201 gcaacgcgtt cagccatcag cgcctgttga ctgaagtgat gatgacgcag catctctgtg
    25261 cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca ccacaccacc ggcatcggta
    25321 tcttcatagt agacgcgaac cggccatcga aacagcgttg tattcacttt acatcccggt
    25381 aatgcaacaa aagttagagc ttttaaacgc tgctactata cgcgcgcgat tgaggtttgg
    25441 gaatgagggc aagttaaggg agcgtaaaaa tttatgggcc tggcacggcc cataattttt
    25501 taaagagaat tctgatttaa aagaagaaga aaatcagccc gacaatgaga acaatatcgg
    25561 caagcaatgg gcaaaaaatc ccttgccaaa gaaccttctg cggacgaaag cccacgccgt
    25621 gaatcacacc agcacagacg gcccacatca gcaataaacc atgccagatt tccagttcac
    25681 tggtcttcgc ggcgaaacga gatgggtccc aaaacataca tcctgctaac agaagcgcca
    25741 tcacgaagga aagcgcccgc agggggcgct tgtccattac cgcatacaag gttgcgataa
    25801 tcttactcat cagatgtctt cttgaccggc tttgcctgat tcgacgtgct caagcgccag
    25861 cgcggtgatt accccaaacg aacaggcaag aagcgttccc agaatccatg cgaaatacca
    25921 catttttagc tccttactta gtacagagag tgggtgttac gttcaatatc ttctttggtg
    25981 atacgaccga acattttcca gtaacaccag gcggtgtaga gcagaatgat cggtaccaga
    26041 accaccgcaa cccaggtcat gacgttaagt gtcagctggc tggaagttgc atcccacatt
    26101 gtcagacttg cgttcatcat ggtgctggac ggcatcacaa acgggaacat tgcgataccg
    26161 gcagtcagga tgatgcaggc cagcgtcagg gaggagaaca caaacgccca cgcggcttta
    26221 tccatacgtg cagtcaggat ggtcagcagc ggcagaacca cacccagtgc cggaatagcc
    26281 cacagaattg gcgtgttgtt gaagttaacc agccatgcgc cagcttcacg aaccacttct
    26341 ttattcagcg ggttagaggc tgcgtaatgg tccattgtcg atttcacgac ataaccatcg
    26401 ataccgtaca tcacccatac gccagccagt gcgaaacaga ccagtgtcac cagtgccgcc
    26461 acctgagccg ttgcacgggt acgcaggtgc agttcgccca cggtacgcat ttgcagatag
    26521 gttgcgccct gagtaatgat catccctacg ctcaccacgc ctgccagcag gccgaacggg
    26581 ttaagcagct ggaagaagtt accggtgtag tacagacgca gatattcatc aacgttgaac
    26641 ggtacgccct gcaacaggtt accgaacgct acaccaatca ccaacggcgg aacgaagcta
    26701 ccaatgaaga tgccccagtc ccacatgtta cgccagcggg tttcttcaat cttggagcgg
    26761 tagtcaaaac cgaccggacg gaagaacaaa gacgccagca cgaggatcat cgccacatag
    26821 aagccggaga acgcagcggc atagaccatc ggccaggcag caaacagtgc gccgcctgcg
    26881 gtgatcagcc aaacctggtt accgtcccag tgtggtgcga tggagttaat cataattcga
    26941 cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc ccacccccat gtcgaaacca
    27001 tcagtgactg caaaaccaat cagcagaacg ccaaccagca gccaccagat aaaacgcaat
    27061 acttcataat cgatcatttg acgactcctg tcttagcgtg ccggctgagt agtcgtggaa
    27121 gactgctcaa agtgatagcg accggttttc aggctgcttg ggccgaggcg tgcaaacttg
    27181 aacattaaga acaattctgc caccaggaac agggtataca ggccgcaaat cagcaccatt
    27241 gagaagatga gatcgcctgc ggtcagtgac gagttcgcca cagctgtcgg cagcacttca
    27301 ccgatagccc acggttggcg gccatattca gccacgaacc agcccgcttc tacagcaatc
    27361 cacggcagcg gaataccgta cagcgcggcg cgcaggagcc attttttctc gccaatgcgg
    27421 ttgcggatga cactccagaa agagagcgcg atgattgcca gcagcaggaa gccacacgcc
    27481 accatgatac ggaacgcgaa gtacagcggc gctacacgcg gaatggagtc tttggttgcc
    27541 tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg tatagcgttt cagcagcaga
    27601 ccgtaaccga ggtccttctt catgctattg aactggtcac gaaccgcctg gtcggtagaa
    27661 ccagaacgca gttgttcgag cagagagtac gccttcatcc cgttacgaat gcgttcttca
    27721 tgctgcacca tcagttcttt caggccgata actggagtat ccacggaacg cgttgcaatt
    27781 atgcccagcg cgtaagggat ctggatcgca aatttgttcg tctcctcttc ctgatcagga
    27841 atgccgaaca gagtaaaggc agcaggcgca ggttgcgttt cccactcagc ttcaatagca
    27901 gccagtttgg ttttctgcac gtcgcccatt tcgtagccgg attcatcacc cagaacaata
    27961 acagacagaa cggcagccat accgaagctg gcagcgatag caaaggagcg tttagcgaag
    28021 gcgaagtcac gacctttcag catataccat gcgctgatac cgaggatgaa catcgcgcca
    28081 gtcacataac cagacgctac agtgtgaacg aatttcacct gagcaaccgg gttaagcacc
    28141 agctcggaga agctcaccat ctccatacgc atagtttcaa agttgaaatc ggacgcgatt
    28201 gggttttgca tccagccgtt cgcaaccaga atccacagtg cggacaggtt tgaaccgagc
    28261 gccaccagcc aggtgacaca catatgctga actttaccca gacgatccca accgaagaag
    28321 aacagaccta caaaggtgga ttcgaggaag aaggccatca gaccttcgat tgccagcggc
    28381 gcaccgaaga tatcccctac atagtgggaa tagtaagacc agttagtccc gaactggaac
    28441 tccatggtca gaccggtagc cacacccaga gcgaagttga taccaaacaa cttgccccag
    28501 aacttggtca tatctttata aatctgtttg ccggaaagga cgtagaccgt ttccataatg
    28561 gccagcagga acgccatacc gagcgtcagt ggcacaaaaa ggaagtggta catcgcggtc
    28621 aaggcaaact gtaagcgcga cagttcgact atatctaaca tcatgactcc ttgctcatcg
    28681 catgaagact ccgagagtga accccgttag aaagggtcac acgcatgccc caatacaaaa
    28741 ttatttgctc gcctctatcg ttgccatttc atcaaaagag gaaaagacaa tgataaaaaa
    28801 g
//
LOCUS       DABSYT020000055        28283 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.055, whole genome shotgun sequence.
ACCESSION   DABSYT020000055 DABSYT020000000
VERSION     DABSYT020000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.055"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(296..2869)
                     /gene="clpB"
                     /locus_tag="ID725_RS14340"
                     /old_locus_tag="pgaptmp_002868"
     CDS             complement(296..2869)
                     /gene="clpB"
                     /locus_tag="ID725_RS14340"
                     /old_locus_tag="pgaptmp_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGV7851416.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2999..3730)
                     /gene="yfiH"
                     /locus_tag="ID725_RS14345"
                     /old_locus_tag="pgaptmp_002869"
     CDS             complement(2999..3730)
                     /gene="yfiH"
                     /locus_tag="ID725_RS14345"
                     /old_locus_tag="pgaptmp_002869"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGV7851417.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3727..4707)
                     /gene="rluD"
                     /locus_tag="ID725_RS14350"
                     /old_locus_tag="pgaptmp_002870"
     CDS             complement(3727..4707)
                     /gene="rluD"
                     /locus_tag="ID725_RS14350"
                     /old_locus_tag="pgaptmp_002870"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGV7851418.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4842..5579
                     /gene="bamD"
                     /locus_tag="ID725_RS14355"
                     /old_locus_tag="pgaptmp_002871"
     CDS             4842..5579
                     /gene="bamD"
                     /locus_tag="ID725_RS14355"
                     /old_locus_tag="pgaptmp_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGV7851419.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5850..6191
                     /gene="raiA"
                     /locus_tag="ID725_RS14360"
                     /old_locus_tag="pgaptmp_002872"
     CDS             5850..6191
                     /gene="raiA"
                     /locus_tag="ID725_RS14360"
                     /old_locus_tag="pgaptmp_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGV7851420.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6294..6419
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6295..6342
                     /gene="pheL"
                     /locus_tag="ID725_RS14365"
                     /old_locus_tag="pgaptmp_002873"
     CDS             6295..6342
                     /gene="pheL"
                     /locus_tag="ID725_RS14365"
                     /old_locus_tag="pgaptmp_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGV7851421.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6441..7601
                     /gene="pheA"
                     /locus_tag="ID725_RS14370"
                     /old_locus_tag="pgaptmp_002874"
     CDS             6441..7601
                     /gene="pheA"
                     /locus_tag="ID725_RS14370"
                     /old_locus_tag="pgaptmp_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGV7851422.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7644..8765)
                     /gene="tyrA"
                     /locus_tag="ID725_RS14375"
                     /old_locus_tag="pgaptmp_002875"
     CDS             complement(7644..8765)
                     /gene="tyrA"
                     /locus_tag="ID725_RS14375"
                     /old_locus_tag="pgaptmp_002875"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGV7851423.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8776..9846)
                     /gene="aroF"
                     /locus_tag="ID725_RS14380"
                     /old_locus_tag="pgaptmp_002876"
     CDS             complement(8776..9846)
                     /gene="aroF"
                     /locus_tag="ID725_RS14380"
                     /old_locus_tag="pgaptmp_002876"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGV7851424.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10056..10421
                     /gene="yfiL"
                     /locus_tag="ID725_RS14385"
                     /old_locus_tag="pgaptmp_002877"
     CDS             10056..10421
                     /gene="yfiL"
                     /locus_tag="ID725_RS14385"
                     /old_locus_tag="pgaptmp_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGV7851425.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10571..11089
                     /gene="yfiR"
                     /locus_tag="ID725_RS14390"
                     /old_locus_tag="pgaptmp_002878"
     CDS             10571..11089
                     /gene="yfiR"
                     /locus_tag="ID725_RS14390"
                     /old_locus_tag="pgaptmp_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGV7851426.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11079..12305
                     /gene="dgcN"
                     /locus_tag="ID725_RS14395"
                     /old_locus_tag="pgaptmp_002879"
     CDS             11079..12305
                     /gene="dgcN"
                     /locus_tag="ID725_RS14395"
                     /old_locus_tag="pgaptmp_002879"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGV7851427.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12321..12803
                     /gene="yfiB"
                     /locus_tag="ID725_RS14400"
                     /old_locus_tag="pgaptmp_002880"
     CDS             12321..12803
                     /gene="yfiB"
                     /locus_tag="ID725_RS14400"
                     /old_locus_tag="pgaptmp_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGV7851428.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITPP
                     "
     gene            complement(12880..13227)
                     /gene="rplS"
                     /locus_tag="ID725_RS14405"
                     /old_locus_tag="pgaptmp_002881"
     CDS             complement(12880..13227)
                     /gene="rplS"
                     /locus_tag="ID725_RS14405"
                     /old_locus_tag="pgaptmp_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGV7851429.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13269..14036)
                     /gene="trmD"
                     /locus_tag="ID725_RS14410"
                     /old_locus_tag="pgaptmp_002882"
     CDS             complement(13269..14036)
                     /gene="trmD"
                     /locus_tag="ID725_RS14410"
                     /old_locus_tag="pgaptmp_002882"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGV7851430.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14067..14615)
                     /gene="rimM"
                     /locus_tag="ID725_RS14415"
                     /old_locus_tag="pgaptmp_002883"
     CDS             complement(14067..14615)
                     /gene="rimM"
                     /locus_tag="ID725_RS14415"
                     /old_locus_tag="pgaptmp_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGV7851431.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14634..14882)
                     /gene="rpsP"
                     /locus_tag="ID725_RS14420"
                     /old_locus_tag="pgaptmp_002884"
     CDS             complement(14634..14882)
                     /gene="rpsP"
                     /locus_tag="ID725_RS14420"
                     /old_locus_tag="pgaptmp_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGV7851432.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15019..16380)
                     /gene="ffh"
                     /locus_tag="ID725_RS14425"
                     /old_locus_tag="pgaptmp_002885"
     CDS             complement(15019..16380)
                     /gene="ffh"
                     /locus_tag="ID725_RS14425"
                     /old_locus_tag="pgaptmp_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGV7851433.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16547..17338
                     /gene="ypjD"
                     /locus_tag="ID725_RS14430"
                     /old_locus_tag="pgaptmp_002886"
     CDS             16547..17338
                     /gene="ypjD"
                     /locus_tag="ID725_RS14430"
                     /old_locus_tag="pgaptmp_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGV7851434.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17360..18645
                     /gene="yfjD"
                     /locus_tag="ID725_RS14435"
                     /old_locus_tag="pgaptmp_002887"
                     /pseudo
     CDS             17360..18645
                     /gene="yfjD"
                     /locus_tag="ID725_RS14435"
                     /old_locus_tag="pgaptmp_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
     gene            complement(18700..19293)
                     /gene="grpE"
                     /locus_tag="ID725_RS14440"
                     /old_locus_tag="pgaptmp_002888"
     CDS             complement(18700..19293)
                     /gene="grpE"
                     /locus_tag="ID725_RS14440"
                     /old_locus_tag="pgaptmp_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGV7851435.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKVKA"
     gene            19416..20294
                     /gene="nadK"
                     /locus_tag="ID725_RS14445"
                     /old_locus_tag="pgaptmp_002889"
     CDS             19416..20294
                     /gene="nadK"
                     /locus_tag="ID725_RS14445"
                     /old_locus_tag="pgaptmp_002889"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGV7851436.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20380..22041
                     /gene="recN"
                     /locus_tag="ID725_RS14450"
                     /old_locus_tag="pgaptmp_002890"
     CDS             20380..22041
                     /gene="recN"
                     /locus_tag="ID725_RS14450"
                     /old_locus_tag="pgaptmp_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGV7851437.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22190..22531
                     /gene="bamE"
                     /locus_tag="ID725_RS14455"
                     /old_locus_tag="pgaptmp_002891"
     CDS             22190..22531
                     /gene="bamE"
                     /locus_tag="ID725_RS14455"
                     /old_locus_tag="pgaptmp_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGV7851438.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22593..22883)
                     /gene="yfjF"
                     /locus_tag="ID725_RS14460"
                     /old_locus_tag="pgaptmp_002892"
     CDS             complement(22593..22883)
                     /gene="yfjF"
                     /locus_tag="ID725_RS14460"
                     /old_locus_tag="pgaptmp_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGV7851439.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22873..23310)
                     /gene="ratA"
                     /locus_tag="ID725_RS14465"
                     /old_locus_tag="pgaptmp_002893"
     CDS             complement(22873..23310)
                     /gene="ratA"
                     /locus_tag="ID725_RS14465"
                     /old_locus_tag="pgaptmp_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGV7851440.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23481..23963
                     /gene="smpB"
                     /locus_tag="ID725_RS14470"
                     /old_locus_tag="pgaptmp_002894"
     CDS             23481..23963
                     /gene="smpB"
                     /locus_tag="ID725_RS14470"
                     /old_locus_tag="pgaptmp_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGV7851441.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24178..24540
                     /gene="ssrA"
                     /locus_tag="ID725_RS14475"
                     /old_locus_tag="pgaptmp_002895"
     tmRNA           24178..24540
                     /gene="ssrA"
                     /locus_tag="ID725_RS14475"
                     /old_locus_tag="pgaptmp_002895"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24809..25057
                     /locus_tag="ID725_RS14480"
                     /old_locus_tag="pgaptmp_002896"
     CDS             24809..25057
                     /locus_tag="ID725_RS14480"
                     /old_locus_tag="pgaptmp_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGV7851442.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
     gene            complement(25173..25325)
                     /locus_tag="ID725_RS14485"
                     /old_locus_tag="pgaptmp_002897"
                     /pseudo
     CDS             complement(25173..25325)
                     /locus_tag="ID725_RS14485"
                     /old_locus_tag="pgaptmp_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(25559..26149)
                     /gene="espM2"
                     /locus_tag="ID725_RS14490"
                     /old_locus_tag="pgaptmp_002898"
     CDS             complement(25559..26149)
                     /gene="espM2"
                     /locus_tag="ID725_RS14490"
                     /old_locus_tag="pgaptmp_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HGV7851443.1"
                     /translation="MPMNTTGMSFSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            26332..26982
                     /locus_tag="ID725_RS14495"
                     /old_locus_tag="pgaptmp_002899"
     CDS             26332..26982
                     /locus_tag="ID725_RS14495"
                     /old_locus_tag="pgaptmp_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGV7851444.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            27061..28119
                     /gene="espW"
                     /locus_tag="ID725_RS14500"
                     /old_locus_tag="pgaptmp_002900"
     CDS             27061..28119
                     /gene="espW"
                     /locus_tag="ID725_RS14500"
                     /old_locus_tag="pgaptmp_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HGV7851445.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNPVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNVRNQNKFPIDVKETVIKLQGQKFTCIEDFYKRYNETRLKANTNIQQEQI
                     ADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRMFENISSDDTDKITGRKFS
                     ELGEYLYSGNVIKLSQLSIRYLPNISSISLIETKQSLLLHRLYSDEVLQRNGTLIPTP
                     LHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSP
                     RNYGLHHNIKTWDPLVRN"
BASE COUNT         7461 a         7247 c         6437 g         7138 t
ORIGIN      
        1 ccgcttcgct ttttctcagc ggcgcggggt gtgcatgata cgctttcccg ctacagagtc
       61 aagcatttat ttttgctttc tctgccggaa ttctcaggag aaccccgctg acccggcggc
      121 gtgtttgccg ttgttccgtg tcagtggtgg cgcattatag ggagttattc cggcctgaca
      181 agcgaaaaat ataaaaactt tatcgttcgc tcatttttca ggcaaaacat cttaaatata
      241 gtcttttccg tctaacttat agacaaaaac gagccccgaa gggctcgttt tatcattact
      301 ggacggcgac aatccggtct tcattaactt ccaggcgaat aactttaccc ggaaccaatt
      361 caccagacag tatttgctgt gccaacgggt tttcgatctg ctgctggata gcacgtttca
      421 aaggacgtgc accatagact ggatcgtaac cgttctcgct cagcagtttc agcgcctcgt
      481 cagaaatgtg gatttcataa ccacgttctt ccagacgttt gtacagacgt ttcaactgaa
      541 tctgcgcaat cgaggcaatg tgctgttcgc ccagcggatg gaagaccacc acttcatcga
      601 tacggttaat gaactccgga cggaagttat ggcttaccac accgagcacc agctctttca
      661 tgtgcgcata atccagttca ccgaagcgtt cctgaatcag atcggaaccg aggttagagg
      721 tcataatgac gaccgtatta cggaagtcga ccgttctccc ttgcccgtca gtcagacgcc
      781 catcatccag tacctgcaac agaatgttga agacatccgg atgcgctttt tccacttcat
      841 ccagcaggat gacggaatac ggacgacgac gcaccgcttc ggtcaggtag ccaccttctt
      901 cataaccgac atatcccgga ggcgcaccaa ccagacgaga caccgagtgt ttctccataa
      961 actcggacat atcgatacgg accatcgcct cgtcgctatc aaacataaag ttcgccagcg
     1021 ccttacaaag ctctgttttc cccacaccag ttgggccgag gaacaggaat gaaccaatcg
     1081 ggcgatttgg atccgccagc cccgcacggc tacgacgaat agcgttagat accgcatcaa
     1141 ccgcttcgtt ctgaccaatt acgcgatggt gcagttcttg ctccatacgc agcagttttt
     1201 cgcgctcgct ttccatcatg cgagaaaccg gaatccccgt ccaacgcgcc agcacttcag
     1261 caatttcggc gtcggtcact ttattacgca acagacgcat agttttgcct tcgagctgcg
     1321 ttgcggcttc cagttgcttt tccagttccg ggattttgcc gtattgcagt tcagacatcc
     1381 gcgccaagtc acccacacgg cgagcctgtt caatagcgat tttcgcctgt tccagttccg
     1441 ctttaatggt ctgcgtacca gaaagcgatg ccttctctgc tttccactct tcttctaact
     1501 cggagtactg acgttctttg tcgctcagtt cttcgttgag catatccaga cgttttttac
     1561 tggcttcatc agactctttc attaacgcct gttgttccag tttgagctgg atgatacggc
     1621 gatcgagtcg gtcgagttct tctggttttg agtcaatctg catacgaatg ctggatgctg
     1681 cttcatcgat caggtcgatg gctttatccg gcagctgacg gtcagcaatg tagcgatgag
     1741 acaacgtcgc cgctgcaaca attgccgggt cagtaatttg cacatggtgg tgcaattcgt
     1801 aacgttcttt caggccacgc agaatcgcaa tggtatcttc aacagaaggc tcggcaacaa
     1861 acactttctg gaaacgacgt tccagcgcag catctttttc gatgtactgg cgatattcgt
     1921 caagcgtcgt ggcacctacg cagtgcaatt caccacgcgc cagcgccggt ttcagcatgt
     1981 ttccggcgtc cattgcgcca tcggctttac ccgcgccgac catggtatgt aattcgtcga
     2041 taaacaggat gacgttgcct tcctgtttgg caagatcgtt aagcacgcct tttaaacgtt
     2101 cttcaaactc accgcgatat ttcgccccag ccaccagcgc gcccatatcc agcgccagta
     2161 cccggcggcc tttcaaccct tccggcactt cgccgttgat aatacgctgc gccagacctt
     2221 caacgatggc agttttaccg acgccgggtt caccaatcag taccgggtta tttttagtac
     2281 gacgttgcag cacctgaatg gtacggcgaa tttcttcatc acgaccaatc accggatcga
     2341 gtttgccctg ttcggctcgt tcggtaaggt cgatggtata ttttttcaaa gcctgacgtt
     2401 ggtcttcagc accttgatcg ttcacgcttt cacctccacg catttgttca atcgcttgag
     2461 taatgttggc ggtggtcgcc cctgctgctt tcaggatgtc ggccagcgtg ccgcgagact
     2521 caagtgccgc cagaacgaac agttctgacg agataaagtt atcaccacgt ttttgcgcca
     2581 gcttgtcgca aagattaaga acgcgcacca gatcctgtga tggctggaca tcaccaccag
     2641 taccttcaac ctgcggtaaa cgatttaatg cctgattgat atctgtgcgc aactggccag
     2701 catttatgcc agcggatgtt aataaaggac taaccgaacc cccttcctga ttcagcaggg
     2761 cgctcattaa atgaagtggt tcgataaatt ggttgtcgtg cccaagtgca agtgattggg
     2821 catcggcaag agcaagctgg aatttattag taagacgatc cagacgcata actcctccca
     2881 taacggatca aagttgctac tggagattaa atgaggtcat ccctcaatta ttcaaggtta
     2941 ttgaccagat taatgtgaaa agaaaatcac gcgtaccgga tcgtcttgat tctttaggtt
     3001 atatcagcca aatgaaactt gccatacgac cggtggtctt gtcgcgacga taagagaaga
     3061 aagtctcatt ttccgtatat gtacaacggt cgccgccgaa aatttgctca acacccacgt
     3121 tcgccagacg ctgccgggca agctgataaa tgtccgccag atacttatca ccatgctgaa
     3181 tgaaagctgt actcgcttcg gcgtctactg ccataaacgc ctcgcgaacc tccgccccca
     3241 cttcaaacgc gcgtgggcca attgccggcc ctaaccaggc gagaatattt tctggattat
     3301 cagcaaaaca ggaaactgtc tcttccagca cgcctgcgca cagtccacgc cagccagcat
     3361 gagcggcggc gacttccgtt cccgctcgat tgcaaaacag cacagggagg cagtcggcag
     3421 tcatcactgc gcaaaccctg ccgggcgtat tgctataaga ggcatctgct cgttttgagg
     3481 cataaggttc gccagtaaga ttaagcacat ctttgccgtg tacctgctca agccagaccg
     3541 gtttagaagg caaattgccc gcagcaaaaa gtcgcttgcg attctcctca acgtgatccg
     3601 ggttatcgcc acaatgggca ccgaggttga gcgagtcata cgggggcaag ctcacgccgc
     3661 cgatacgagt agagctacag gccgcaacac cttttggcag cggccactgc gggacaatca
     3721 gcttactcat aaccagtcca cttcatcttt atgctcttcg aaatcggcgc gcatcacctc
     3781 aatcagctcc accatatctt gtggaatagg cgcatgccat tccatttcga taccggagat
     3841 cgggtgataa agacgcagca tggttgcatg aagcgcctgg cggtcaaatt tacgcagcgt
     3901 ggagataaac gcttccgaag caccttttgg cgggcgcgga cggccaccat aaaccggatc
     3961 gcccaccagc ggatgagtga tatgggccat atgcacgcgg atctggtgcg tacgtccagt
     4021 ttccagacgc aaccgcagac gcgtgtgcac acggaagtgt tccatgatgc gatagtgagt
     4081 caccgccggt ttgcccatcg gatgcaccgc catgtgagta cgtttggtcg ggtggcggct
     4141 gattggttca tctactgtac cgccagcggt catatgacca attgccaccg cttcatactc
     4201 acgagtaatt tcacgccttt gcaaagattc gactaaacgc gtctgagccg gaacggtttt
     4261 tgccacaacc atcaggccag tggtgtcttt atccagacga tggacgatgc ctgcacgcgg
     4321 tacatcagca atgggcggat agtaatgaag taacgcattc agtaccgtgc catccgggtt
     4381 acccgcgcca ggatgtacca ccagatcgcg cggtttatta atgacgatga tatcttcatc
     4441 ttcatagacg atatccagcg ggatatcctg cggttcaaaa cgcgcttctt cttcaatctc
     4501 agcgttgatg gcaacctgct cgccacccaa tactttttct ttcggcttat cacaaagttt
     4561 gccgttaacc agcacgcgct ggtcgaggat ccattctttt attcgcgaac gtgaataatc
     4621 cgggaacatt tcggccaaag cctgatctaa gcgttgaccg agttggtttt cggacaccgt
     4681 tgcagtgagc tgtactcgtt gtgccatata tactgcttct tcgtttatcg ttggggtttt
     4741 acggctttgc cgtttaatat agtgtgctat tgtagctggt cttaaccggg agcaggaaca
     4801 gagaatctcc cgtattacat tttgaggaaa gtcaaaacgt catgacgcgc atgaaatatc
     4861 tggtggcagc cgccacacta agcctgtttt tggcgggttg ctcggggtca aaggaagaag
     4921 tacctgataa tccgccaaat gaaatttacg cgactgcaca acaaaagctg caggacggta
     4981 actggagaca ggcaataacg caactggaag cgttagataa tcgctatccg tttggtccgt
     5041 attcgcagca ggtgcagctg gatctcatct acgcctacta taaaaacgcc gatttgccgt
     5101 tagcgcaggc tgccatcgat cgttttattc gccttaaccc gacccatccg aatatcgatt
     5161 atgtcatgta catgcgtggc ctgaccaata tggcgctgga tgacagtgcg ctgcaagggt
     5221 tctttggcgt tgaccgtagc gatcgcgatc ctcaacatgc acgagctgcg tttagtgact
     5281 tttccaaact ggtgcgcggc tatccaaaca gtcagtacac caccgatgcc accaaacgtc
     5341 tggtattcct gaaagatcgt ctggcgaaat atgaatactc cgtggccgag tactatacag
     5401 aacgtggcgc atgggttgcc gtcgttaacc gcgtagaagg catgttgcgc gactacccgg
     5461 atacccaggc tacgcgtgat gcgctgccgc tgatggaaaa tgcataccgt cagatgcaga
     5521 tgaatgcgca agctgaaaaa gtagcgaaaa tcatcgccgc aaacagcagc aatacataac
     5581 agaaacctga aacacaaaac ggcagccctt gagctgccgt ttttttattc tgtcagttgt
     5641 gaaactgaag cgatttagtc actatcgatc tcatcaaata tggctcgctt tgagatattc
     5701 ctcaagtaaa aaaacacctc ttcctgcgat ttctcacaaa aaagattcgt tgacaaaaag
     5761 tgacaaaatt atgagatttc catcacacat tttgacatca ggaacggtat gctgaattca
     5821 ccaagacggg aagacaagag gtaaaattta tgacaatgaa cattaccagc aaacaaatgg
     5881 aaattactcc ggccatccgc caacatgtcg cagaccgtct cgccaaactg gaaaaatggc
     5941 aaacacatct gattaatcca catatcattc tgtccaaaga gccacaaggg tttgttgctg
     6001 acgccacaat caatacacct aacggcgttc tggttgccag tggtaaacat gaagatatgt
     6061 acaccgcaat taacgaattg atcaacaagc tggaacggca gctcaataaa ctgcagcaca
     6121 aaggcgaagc acgtcgtgcc gcaacatcgg tgaaagacgc caacttcgtc gaagaagttg
     6181 aagaagagta gtcctttata ttgagtgtat cgccaacgcg ccttcgggcg cgttttttgt
     6241 tgacagcgtg aaaacagtac gggtactgta ctaaagtcac ttaaggaaac aaacatgaaa
     6301 cacacaccgt ttttcttcgc attctttttt accttcccct gaatgggagg cgtttcgtcg
     6361 tgtgaaacag aatgcgaaga agaacaataa ggcctcccaa atcggggggc cttttttatt
     6421 gataacaaaa aggcaacact atgacatcgg aaaacccgtt actggcgctg cgagagaaaa
     6481 tcagcgcgct ggatgaaaaa ttattagcat tactggcaga gcggcgcgaa ctggccgtcg
     6541 aggtgggaaa agccaaactg ctctcgcatc gcccggtacg tgatattgat cgtgaacgcg
     6601 atttactgga aagattaatt acgctcggta aagcgcacca tctggacgcc cattacatta
     6661 ctcgcctgtt ccagctcatc attgaagatt ccgtattaac tcagcaggct ttgctccaac
     6721 aacatctcaa taaaattaat ccgcactcag cacgcatcgc ttttctcggc cccaaaggtt
     6781 cttattccca tcttgcggcg cgccagtatg ctgcccgtca ctttgagcaa ttcattgaaa
     6841 gtggctgcgc caaatttgcc gatattttta atcaggtgga aaccggccag gccgactatg
     6901 ccgtcgtacc gattgaaaat accagctccg gtgccataaa tgacgtttac gatctgctgc
     6961 aacataccag cttgtcgatt gttggcgaga tgacgttaac tatcgaccat tgtttgttag
     7021 tctccggcac gactgattta tccaccatca atacggtcta cagccatccg cagccattcc
     7081 agcaatgcag caaattcctt aatcgttatc cgcactggaa gattgaatat accgaaagta
     7141 cgtctgcggc aatggaaaag gttgcacagg caaaatcacc gcatgttgct gcgttaggaa
     7201 gcgaagctgg cggcactttg tacggtttgc aggtactgga gcggattgaa gcgaatcagc
     7261 gacaaaactt cacccgattt gtggtgttgg cgcgtaaagc cattaacgtg tctgatcagg
     7321 ttccggcgaa aacgacgttg ttaatggcga ccgggcaaca agccggtgcg ctggttgaag
     7381 cgttgctggt actgcgcaac cacaatctga ttatgacccg tctggaatca cgcccgattc
     7441 acggtaatcc atgggaagag atgttttatc tggatattca ggccaatctt gaatcagcgg
     7501 aaatgcaaaa agcattgaaa gagttagggg aaatcacccg ttcaatgaag gtattgggct
     7561 gttaccctag tgagaacgta gtgcctgttg atccaacctg atgaaaaggt gccggatgat
     7621 gtgaatcatc cggcactgga ttattactgg cgattgtcat tcgcctgacg caataacacg
     7681 cggctttcac tctgaaaacg ctgtgcgtaa tcgccgaacc agtgctccac cttgcggaaa
     7741 ctgtcaataa acgcctgctt atcgccctgc tccagcaact caatcgcctc gccgaaacgc
     7801 ttatagtaac gtttgattaa cgccagatta cgctctgacg acataataat gtcggcataa
     7861 agctgcggat cctgagcgaa cagtcgcccg accatcgcca gctcaaggcg gtaaatcggc
     7921 gaagagagcg ccagaagttg ctcaagctga acattttctt ctgccaggtg cagcccgtaa
     7981 gcaaaagtag caaagtggcg cagtgcctga ataaacgcca tattctgatc gtgctcgacg
     8041 gcgctaatac gatgcaaccg agcgccccag acctgaattt gctccagaaa ccattggtat
     8101 gcttccggtt tacgtccatc acaccagacc acaacttgct ttgccaggct accgctgtcc
     8161 ggaccgaaca ttgggtgtaa ccccagtacc gggccatcgt gcgccgccag catggcctgt
     8221 aatggtccat ttttcactga tgccagatca accagaatac aatctttcgg taaaggcggt
     8281 aatttgccaa taacttgctc agtaacgtgg attggcacac taacaatcac cattccggca
     8341 tcggcaacaa tatcagccgc tcgatcccag tcatgttgct ccagaatccg cacctgataa
     8401 cccgagagtg tcagcatctt ctcgaacaga cgtcccatct gaccgccgcc gccaacgata
     8461 actaccgggc gtaacgcagg acaaagcgtt ttaaatcctt tgtcgttttc actggagtaa
     8521 gattcacgca tcacccgacg caaaacatcc tcaatcagat ctggcggtac acccagagct
     8581 tccgcctctg cacgacgcga ggccaacata gatgcctctc gctccggaac ataaataggc
     8641 agtccaaagc ggcttttcac ctcgcccact tcagcaacca gttccagacg cttcgctaat
     8701 aaattcagca gcgctttatc gacttcatca atttgatcgc gtaatgcggt caattcagca
     8761 accataataa acctcttaag ccacgcgagc cgtcagctgc ccgttcagat cctgatgaat
     8821 ttcacgcagc aaggcatcgg tcatttccca gctaatgcag gcatcggtta cggatacacc
     8881 gtatttcatt tcactgcgcg gttgctcgga agactgattg ccctcgtgga tattactttc
     8941 gatcatcaga ccaataattg agcgattgcc atctttgatt tgagcaacca cggattctgc
     9001 caccgcaggc tgacggcgat aatctttatt ggaattaccg tggctgcaat ctaccatcag
     9061 agacgggcgc agtcccgcct gttccatctc tttttcacat tgcgcaacat ccgcagggct
     9121 atagttcggc gctttaccac cgcgcaggat gacatgcccg tccggattcc cctgagtttg
     9181 tagcaacgca acctgccctg cctggttaat gccaacaaaa cggtgcggct gggcggcggc
     9241 gcgcatagcg ttaattgctg ttgccagact gccgtcggtg ccgtttttaa agccaaccgg
     9301 catggaaagc ccggaggcca tttcacggtg agtttgcgat tccgttgtac gagcaccaat
     9361 tgctgaccag ctaaacagat cgcccaggta ttgcgggcta ttcggatcta acgcttccgt
     9421 cgccagtggc agtcccatat tcaccagctc aagcagcaat ttacgcgcga tctgcagccc
     9481 ggcttctaca tcaaaagagc catccatatg gggatcgtta attaaccctt tccagccgac
     9541 agtggtacgg ggtttttcaa aatagacgcg cattaccaga tagaggctat cgctgacctc
     9601 tgcggcaagg gctttaaatc gacgagcata ttccagagca gtttccggat catgaatgga
     9661 acaagggcca catactacca gcagacgagg atcgcgcccg gcgataatat ctgaaatggt
     9721 tttacgcgag tcagcaatct gggcttcttg ttgcaggctt aatggaaaag cggccttcag
     9781 ttgtgccgga gtcattaaaa cctgttcgtc ggtaatatgt acgttattca gcgcgtcttt
     9841 ttgcatgatg gcgatcctgt ttatgctcgt ttgcgatagt tgatcctcag cgaggatgac
     9901 gtaacgataa cacataaagt aaagttttca atccatattt cgtacatttt tctttacata
     9961 agcaatttat tcgcgctttc aacccttgat tctgtataga taaatttaca ccacctttga
    10021 aaacaatccg ctatgctttg aaaaaggaga aagaaatgat gaaaaagttt atcgccccct
    10081 tgttggcttt actggttagc ggatgccaga ttgatcctta tactcacgcg ccaaccttga
    10141 ccagcaccga ctggtatgat gttggtatgg aagatgcgat atcgggcagc gccataaaag
    10201 atgacgatgc atttagcgat tcacaggcgg atcgcggtct ataccttaaa ggatatgccg
    10261 aaggacaaaa gaaaacttgc cagaccgatt ttacttatgc ccgaggactt tccggtaaaa
    10321 gctttcctgc cagctgcaac aatgttgaaa atgccagcca actgcatgaa gtatggcaaa
    10381 aaggggctga tgaaaatgcc agcgcaatac gattaaatta attaaaaccc cacaaataaa
    10441 ttaaacataa gattttactc attatgtttt atttctcaca atgatgacga ccgcatgaaa
    10501 taatgatatt ttaatgagca ttctgaaaaa atatcgatgc atttcgagcg aagatggtgg
    10561 ggatccctga atgcgttttt ctcaccgact gttcttactg ctgattctcc tgctgacggg
    10621 agcaccaata ctcgctcagg agccttctga cgttgcgaaa aacgtgcgta tgatggtttc
    10681 cggtattgtc agttacaccc gctggcctgc gctatcaggg ccaccaaagt tatgtatctt
    10741 ttcatcttca cgttttagta ccgccctaca agagaatgcc gcaacatctt tgccttatct
    10801 gcctgtcatc attcacactc aacaagaggc gatgatttca ggctgcaatg gtttttattt
    10861 tggcaacgag tcaccgacat tccagatgga attaaccgaa caatatccgt caaaggcgtt
    10921 attattaatt gccgaacaaa ataccgagtg cattattggt agcgcctttt gtctgatcat
    10981 ccacaataat gacgtcagat ttgccgtaaa cctggatgcc ttatcgcgta gcggtgtaaa
    11041 ggtcaacccg gatgtcttaa tgctcgcacg gaagaaaaat gatggataac gataattctc
    11101 ttaataagcg ccccacgttt aaaagagcat tacgcaacat cagtatgacc agcatattta
    11161 tcactatgat gctgatctgg ttgctgcttt ccgtgacctc ggtgctgccc ctgaaacagt
    11221 acgcgcaaaa aaacctggca ctgacagcag caacaatgac ttacagtctg gaagcagctg
    11281 tcgtttttgc cgatggccct gcagcaactg aaacactggc agcgctgggc cagcaagggc
    11341 aattttcaac tgcagaagta cgtgataagc agcaaaatat tctggcatcc tggcattaca
    11401 cccgtaagga tccaggcgat actttcagca atttcataag ccactggctc ttccctgccc
    11461 ccatcattca gccgattcgt cacaatggtg aaaccattgg cgaagtacgc ttaaccgctc
    11521 gcgacagttc aatcagccat tttatctggt tttcgctcgc cgtactgacc ggttgtattc
    11581 tgctggcatc aggaatcgca attaccctca cccgccattt gcacaatggc ctggtggaag
    11641 cactgaaaaa tatcaccgat gtcgtacatg atgtgcgttc caaccgcaat ttttcccgac
    11701 gagtttcgga agaacgtatc gctgagtttc accgcttcgc tctcgacttc aacagtctgc
    11761 tggatgaaat ggaagagtgg cagcttcgtt tacaggccaa aaatgcgcag cttctacgta
    11821 ccgcgctaca tgacccatta accgggctgg ctaaccgcgc agcgtttcgt agcggcatca
    11881 atacgttgat gaacaattcc gctgcccgaa aaacgtcggc gttactattt cttgatggcg
    11941 ataatttcaa atatatcaat gatacctggg gtcatgcgac gggcgataga gtcttgattg
    12001 aaatcgcaaa acggttagct gaatttggcg ggctgcgaca taaagcatac cgcctgggcg
    12061 gcgatgaatt cgctatggtg ctctatggtg tacagtcgga atctgaagtg cagcagatat
    12121 gctcagcact gacacaaatc tttaatctcc cgtttgatct tcataatggc catcagacca
    12181 ccatgacatt aagcattggt tacgcaatga ccattgcgca cgcttctgcg gaaaaattac
    12241 aagagctggc cgatcacaat atgtatcagg ccaaacacca gcgtgccgaa aagctggtga
    12301 gataacaagg atatatcgat atgataaagc acctggtagc acccctgatt ttcacctcac
    12361 taatactgac tggctgccag tcccctcagg gaaagtttac tcctgagcaa gtcgccgcta
    12421 tgcaatctta tggatttact gaatccgccg gcgactggtc gctgggctta tcagatgcca
    12481 ttctgttcgc aaaaaatgac tacaaattgc tcccggaaag ccagcaacag atccaaacca
    12541 tggcagccaa attggcctcg acagggctaa cacatgcccg tatggatgga cacaccgata
    12601 actatggtga agacagttac aacgaaggct tatcattgaa acgggcgaat gtcgtggccg
    12661 atgcatgggc tatgggtgga caaattccac gcagcaatct caccacacag ggtttaggaa
    12721 aaaaatatcc catagccagt aacaagaccg cccagggccg cgccgagaac cgccgcgtcg
    12781 cagtggtgat tactccccct taaaacacga gcaaacagat aaaaaaaggg ccagccaatt
    12841 ggccagccct tcttaacagg atgtcgctta agcgaaatct tagttaagac gctctttgat
    12901 acgagcagcc ttaccagtac gctcacgcag gtagtacagt ttagctttac gaacagcacc
    12961 acgacgtttg acagaaatgc tgtcaactac cggagagtga gtctggaaga cacgctcaac
    13021 gccttcgccg ttggaaattt tacgaacagt gaatgcagag tgcagaccgc ggttacgaat
    13081 agcgataacc acgccctcga atgcctgcag acgttttttg gaaccttcaa caacccatac
    13141 tttcacttcc acggtatcac ccggacggaa ggaaggtacg tcctgcttca tctgctcttg
    13201 ttcaagttgc ttaataatgt tgctcataat ttaatctctt atcctgggta aactgatatc
    13261 tcgggggctt acgccatccc atcatgttta tgttgctgtt gtgcgtgttc cgttttgaac
    13321 tccgccagca accttgcttg ctcttcagtc agagccaggt tttccagaag ttcaggtctt
    13381 ctaagccagg tacggcccag cgactgtttc aaacgccagc gacgtatctc ggcatggttg
    13441 cccgacagta acactggcgg aacttccatc ccttctaaca cctcaggccg cgtatagtgc
    13501 gggcaatcca gcaatccttc agcaaaggaa tcttccgttg ccgaggcttc atgtcccagt
    13561 actcccggaa taaaccggga aacggagtca atcagcgtca ttgctggtaa ctcaccacca
    13621 ctgagaacgt aatcgccgat tgaccattct tcgtcaattt cggtttggat cacgcgctca
    13681 tctatacctt cgtagcgacc gcacaccaga atcaattttt gattcgttgc cagttcgctg
    13741 acgcccgctt gatcaagctt gcgtccctgt ggtgaaagat aaatcacctt tgcgccttca
    13801 cccgccgcgg cttttgctgc atgaatggcg tcccgcaagg gttgcaccat cattaacatc
    13861 cccggtccgc cgccgtaagg acgatcgtcc acggtacggt gccggtcatg cgtgaaatcg
    13921 cgaggactcc agctctggat gctcagcagg ccatttttaa ctgcccggcc agttaccccg
    13981 taatcggtaa ttgcgcggaa catttcagga aacaggctaa ttatgccaat ccacatagcg
    14041 ccgtctttta ccgtttatcc ggtggtttaa aaaccaggat cccaatctac ttcgattgaa
    14101 cgagtagtga gatcgacttt cttgataacc tgcccatcga ggaacggtac gagacgttcc
    14161 ttgataccaa acgcatcttt caggtttgcc ttaatgacga gaacgtcatt ggatccggtt
    14221 tccatcatat cgacgacttt accgagatcg tagccttcag tggttactac ctggcagccc
    14281 atcaggtctt tccagtagta gtcaccctct tcaagctgag gcagctgcga tgaatccacg
    14341 acaatttcac aattcgtcag caggttcgcc gcatcacgat cgtcaacgcc tttcagcttg
    14401 atgatcatgt cctgattgtg gtgcttccag ctttccagct ggacttgctg ccactgaccc
    14461 gccttctgga taaaccaggg ctgatagtca aaaatgcttt cggcgtcttc ggtggaagaa
    14521 aacactctga gccacccacg aataccgtaa gacgaaccca tttttcccaa aacgatggga
    14581 tcaacaggtg cttgcgcggt gagttgtttg ctcatcatga ccaccgtgac agattaagct
    14641 gctttgttta cttctttgat cagcgcagca acgcgatcag aaatagttgc gccctggcca
    14701 acccagtgag cgatgcgatc cagatccagg cgagtgcctt cttctttttc gctagcgatt
    14761 gggttgaaga aaccaacgcg ctcgatgaag cgaccgttgc gtgcattacg gctgtcagcg
    14821 acaacaacct ggtagaacgg acgcttttta gcgccgtgac gtgctaaacg aatagttacc
    14881 ataacatcct cttgtgtgaa taaaacaacc ggaccccatc gaggaacgga gtccggtgtc
    14941 atattaaaag cccgaaaatt ttactgattt ctggagaaaa tgcaatcagc agttaataac
    15001 tctgtcgcat aaggtcattt agcgaccagg aaatcccggc ggcatcatcc ccttcatgct
    15061 tctcatcatc ttcgccattc cgcccttctt cattttcttc atcatgcgct gcatgtcgtc
    15121 gaactgtttc agaagacggt taacgtcctg cacctgcata ccgcaaccgg cagcaatacg
    15181 gcgtttacgc gaacctttga tgatttctgg cttagcgcgc tctttcatcg tcatcgagtt
    15241 gatgatggct tccatacgca ccagcacttt atcgtccatc tgtgacttga cgttatccgg
    15301 gatctgcccc atgcccggca gtttgcccat caggctggcc atgccgccca tatttttcat
    15361 ctggcgcagt tgctcaagga agtcgttgag atcgaagccg tcaccttttt tcagcttgct
    15421 ggctaatttc tctgcctgcg cgcggtcaac tttgctttcg atatcttcga tcagcgacag
    15481 tacgtcgccc atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc
    15541 ctcagttttc tcgccaacac cgaggaactt gatcggtttg ccagtgatgt gacgaataga
    15601 gagcgccgca ccgccgcggg catcgccgtc cactttggtc aacactacgc cggtaagcgg
    15661 taacgcttca ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac
    15721 cacaaacagg gtttcaaccg ggttaatcga cgcatggact tgtttgatct cgtccatcat
    15781 cgcttcgtca acgtgcagac gaccagcggt atccaccagc agcacgtcgt agaatttcag
    15841 tttggcttct ttcagcgccg cgttaacaat atcaaccggc ttctgaccaa catcagaagg
    15901 gaagaaatca acgcccacct gctctgccag cgtctcaagc tgtttgattg ccgccgggcg
    15961 atagacgtca gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca ggaacttacc
    16021 gagtttacca acgctggttg ttttaccggc accttgcagg cctgccatca gcacgaccgc
    16081 aggcggttgc gcagccagat tcagggtctg gttctcttcg cccatcgccg caaccagttc
    16141 gttacggacg attttgacga actcctgccc cggcgtcagg ctcttattaa cttcatgacc
    16201 aaccgctttc tcttttacgc gattgataaa ctcacgcact accggcagag cgacgtccgc
    16261 ctccagcagc gccatgcgca cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg
    16321 tccacggcca ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat
    16381 tgtctctcgc ctggggtgga aacggttggc cgcaatcgcg acacatcatc agtattttgc
    16441 cgcagtataa catgaaggcg tctttgttgt tatgcaacgg ttggagcagc gttcacctga
    16501 cgctatactg cttctctttc ttattgctca aactgtcgac atcactatgc ccgtttttgc
    16561 tctgctcgcg cttgtcgcct actccgtcag tcttgcgctg attgttcccg gtctgctgca
    16621 aaaaaacggc ggctggcggc gcatggctat tatttctgcg gtcattgcgc tggtctgcca
    16681 cgcaatcgct ctggaagccc gcatcctgcc cgacggtgat agcggacaaa acctcagcct
    16741 gctgaacgtt ggttcattgg tcagtttgat gatctgtacg gtaatgacca ttgtggcttc
    16801 tcgcaatcgt ggctggttgc tgctacccat tgtctatgcc tttgcgctta tcaacctggc
    16861 gctggcaacc ttcatgccca atgaatacat cacccatctg gaagctacgc ctgggatgct
    16921 ggtgcacatt ggcttatcgc tcttttccta tgccacgcta attatcgccg ccctgtacgc
    16981 gctgcaactg gcgtggattg attaccaact gaagaacaag aagctggcgt ttaaccagga
    17041 aatgccgcca ttgatgagta tcgagcgtaa aatgttccac atcacgcaga ttggcgtggt
    17101 gctgctaacg ctcacgcttt gcactggcct gttctacatg cacaacttat ttagcatgga
    17161 aaatatcgac aaggctgtgc tctctatcgt ggcgtggttt gtctatattg tgctgctatg
    17221 gggacattat catgaaggat ggcgtggacg tcgcgtcgtc tggtttaacg ttgcgggtgc
    17281 agtcattctg acactggcct acttcggcag ccgaattgtc cagcagttaa tcagctaaac
    17341 ccagaaaagg agtttccccc tggaacacat ttctactact acgttgatca ttattctgat
    17401 catcatggtg gtcatttcag cctatttttc cgggtccgaa accggaatga tgaccctcaa
    17461 ccgctatcgt ctgcgacata tggcgaaaca gggtaatcgc tcggccaaac gcgtcgaaaa
    17521 attgctgcgt aagccagacc gcctgataag cctggtgtta atcggcaata acctggtcaa
    17581 tattcttgcc tccgcgctcg gcactattgt tgggatgcgt ttgtacggcg atgcgggcgt
    17641 ggcaattgcg actggtgtgc tgacttttgt cgtactggtt tttgctgagg tattgccgaa
    17701 aaccattgcc gcgctgtacc cggaaaaagt cgcttatccg agtagttttc tgctggctcc
    17761 cctgcaaatt ttgatgatgc cgctggtctg gttgctgaat gctatcaccc gtatgctgat
    17821 gcgcatgatg ggtatcaaaa ccgatatcgt ggttagcggc tctttgagca aagaagagtt
    17881 gcgcactatc gtgcacgaat cgcgctcaca aatttcccgt cgcaatcagg atatgctgct
    17941 gtcggtgctc gatctggaaa aaatgaccgt tgatgacatc atggtgccgc cagtgaaatt
    18001 atcggtattg atatcaacga tgactggaaa tctattctgc gccaactctc ccactcacct
    18061 cacgggcgca tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat gctgcgagta
    18121 cgtgaagctt ggcggttgat gtcggagaaa aaagagttca ccaaagaaac catgctgcgc
    18181 gccgcggacg agatctattt tgtgccggaa ggtacgccgc tcagcacgca gttggtgaag
    18241 tttcagcgca acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga cattcagggg
    18301 ctggtgacgg ttgaagatat tctggaagag attgtcggcg atttcactac gtcgatgtcg
    18361 ccaacacttg ccgaagaagt cacgccgcaa aacgacggtt cggtgattat tgatggcact
    18421 gccaacgtgc gggaaatcaa caaagccttt aactggcatc taccggaaga tgatgcccgc
    18481 acggttaatg gcgtcattct tgaggcactg gaagagatcc ctgtcgtagg cacccgcgtg
    18541 cgtattggcg agtacgatat cgatattctc gacgtacagg acaatatgat taagcaggta
    18601 aaagtttttc ctgtgaaacc gctgcgcgag agtgtggcgg agtaacgaaa acggcccggc
    18661 attcgcatgc agggccgtga attattacga aaacagaaat tacgctttta ctttcgctac
    18721 agttaccatc gccgcacgaa tcgtacgacc attcagcgta taacccttct gcataatgcc
    18781 cagtacgtta cctggcgcaa cgtcatcaga ttccaccatt gcgatagcct gatgcacatt
    18841 cgggtccagc ggcacgttag tttcggcgat cacttcaaca ccaaacttac gcacaacatc
    18901 cagcatcgac ttcagcgtca gctcaatgcc ttcaaccatc gcagacatat ccgggttagc
    18961 tttatcagcc acttccagcg cacgatccag gctatcaatc accggcagca attcgttgat
    19021 gaatttctcc agcgcgaatt tgtgggcttt ttcaatatcc agttcagtac gacgacgcag
    19081 gttttccatt tcggctttta cacgcaaaat gccgtcacgt tcacgggtct gggcttcagc
    19141 cagctgagct tcgagattcg caattttttc atcgcgcgga tccacctgct cagcagaagc
    19201 ttctggctca actgcctcaa tctcttcgtg ctgatccatg ataatttctt ccggggcttg
    19261 cccctcaggc gttttctgtt ctttactact catgaatttc tccgcgtttt tttcgcattc
    19321 atctcgctaa cttcgcttat tatggggatc agtttcaggg tttcaaggga agcactcaca
    19381 ttgtcatcaa tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc aagtgtattg
    19441 gcattgtggg acacccacgg caccctactg cactgacaac acatgaaatg ctctaccgct
    19501 ggctgtgcac aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac gaactgcaac
    19561 tgaagaatgt gaaaactggc acgctcgcgg agattgggca actggctgat ctcgcggtag
    19621 tcgttggtgg cgacggtaat atgctgggcg cgacgcgcac gctcgcccgt tacgatatta
    19681 aagttattgg aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac cccgataacg
    19741 cccagcaaca attagccgat gtgctggaag gtcactacat cagcgagaaa cgttttttgc
    19801 tggaagcgca agtctgtcag caagattgcc aaaagcgcat cagcaccgcg ataaatgaag
    19861 tggtacttca tccaggcaaa gtggcgcata tgatagagtt cgaagtgtat atcgacgaga
    19921 tctttgcgtt ttctcagcga tctgatgggc taattatttc gacgccaaca ggctccaccg
    19981 cctattccct ctctgcgggc ggtcctattc tgaccccctc tctggatgcg attaccctgg
    20041 tgcccatgtt cccgcatacg ttgtcagcac gaccactggt cataaacagc agcagcacga
    20101 tccgtctgcg tttttcgcat cgccgtaacg acctggaaat cagttgcgac agccagatag
    20161 cactaccgat tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac catctgaatc
    20221 tgattcatcc gaaagattac agttatttca acacattaag caccaagctc ggctggtcaa
    20281 aaaaattatt ctaattttac gccagcctct ttactgtata taaaaccagt ttatactgta
    20341 cacaataaca gtaatggttt ttcatacagg aaaacgacta tgttggcaca actgaccatc
    20401 agcaactttg ctatcgttcg tgagcttgag attgattttc atagcggcat gaccgtaata
    20461 actggcgaaa ccggtgcggg taaatctatt gcaatagatg ccctcggtct ttgtctcggt
    20521 ggtcgcgctg aagccgacat ggtgcgtacc ggcgctgctc gcgctgacct gtgcgcccgt
    20581 ttttctctga aagatacgcc agcggccctg cgctggctgg aagaaaacca gcttgaagac
    20641 gggcatgaat gtttgcttcg tcgcgtgatc agcagcgatg gtcgctcccg tggtttcatc
    20701 aacggtacag ctgttcctct gtcacaactg cgcgaactgg gtcagttgct gattcagatc
    20761 catggtcagc acgctcatca gttactcacc aaacccgagc accaaaaatt cctgcttgat
    20821 ggctatgcca atgaaacctc tctactccag gaaatgaccg cacgttatca gttgtggcat
    20881 caaagctgcc gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc tgcccgtgcg
    20941 gaactgctgc aataccaatt aaaagaactt aacgaattta atccgcagcc cggagagttt
    21001 gagcaaatcg acgaagagta caaacgtctg gcgaacagcg gtcaattgct gaccaccagc
    21061 cagaatgcat tggcattaat ggccgacggt gaagacgcaa acctgcaaag tcagctttac
    21121 acggctaaac aactggtgag cgaattgatt ggcatggaca gcaaactgtc cggcgtactt
    21181 gatatgctgg aagaagctac catccagatt gttgaagcca gcgacgaact gcgccactac
    21241 tgcgatcgtc tggatctcga tcccaaccga ctgtttgaac ttgaacagcg catctcaaaa
    21301 cagatttcgc tggcacgtaa acatcacgtc agccctgaga cattgccaca gtattaccag
    21361 tcgctactgg aagaacagca gcaactggac gatcaggccg actcacaaga aacgctcgca
    21421 ctggcggtaa cgaaacatca tcagcaggca ctggaaacgg cgcgcgcatt acaccagcaa
    21481 cgccagcatt atgcagaaga acttgcacag ctgatcaccg acagtatgca tgcgctctca
    21541 atgccgcatg ggcagtttac gattgatgtc aaatttgacg agcatcacct gggcgctgac
    21601 ggcgccgatc gtattgagtt tcgggtaacc accaacccag gtcagccaat gcagcctatt
    21661 gccaaagtcg catccggtgg tgaattgtcc cgcatcgcat tggcaattca ggtcatcacg
    21721 gcgcgtaaaa tggaaacccc ggcactgatt tttgatgaag tggatgtagg gattagcggt
    21781 ccaacagcgg cagttgtcgg caaactgctg cgtcaactcg gcgaatcaac tcaggtgatg
    21841 tgtgttaccc acctgccaca agtcgcggga tgtggtcatc aacactattt tgtcagcaaa
    21901 gaaaccgatg gtgcgatgac agaaacgcat atgcaatccc tgaataaaaa aacgcggtta
    21961 caagagctgg cgcgcctgct tggtggcagt gaagtcacac gtaacacact ggcgaatgcg
    22021 aaagaactgc ttgcggcgta aacttttttc ctgcttcacg gtcagagtaa acagcaaaac
    22081 gccgtaagac cggaaagcaa aaggttttaa agtgatgaaa ggtctattat catcggcata
    22141 ttacagatga gccacgtact gctcgggccc gaaaaggaat caaatcacta tgcgctgtaa
    22201 aacgctgact gctgcagcag cagtactatt gatgttgacc gcaggctgtt ccactctgga
    22261 gcgagtggtt taccgtcctg acatcaacca ggggaactat ctgaccgcta acgacgtatc
    22321 caaaatacgt gttggcatga cgcaacaaca agttgcgtac gcattgggta caccgctgat
    22381 gtccgatcca tttggtacga atacctggtt ctatgtcttc cgccagcaac caggtcatga
    22441 aggtgtaacg cagcaaacgc tgacgctaac ctttaacagt agcggtgtgt tgaccaatat
    22501 tgataacaaa cctgcgctga gtggtaacta ataataaagt tgctctcaaa gacgttaaaa
    22561 aaggtgctca atgagcacct tttttctgtc cgttatttat tcgctgattt ttctgctcgt
    22621 tgcctgcgaa gctctttcgg atcggcaatg agagggcgat aaatctccac ccgatcgcca
    22681 tcatgcacac tatcgcttaa ttttgccgga cggctgtaaa tgccgacttt gttttcagtt
    22741 aaatcgatat cggtacgcaa ttccagcaag ccactggcgc gaatagcttc ttcaaccgtc
    22801 gcgccctcct gcagcgtcac gcgctgcagg tactgcttct taggtagcgc ataagccacc
    22861 tcaacggcaa ttttacctgg cactgtaaac ctctttcgca cgaaccgtaa aagcctggac
    22921 catattagac gccagctctt taaacacgcg accaaaagcg agttcaatca acttattggt
    22981 aaactcaaag tcgagatgaa actcgatacg acacgcctcc tggctcagcg gcgtaaactt
    23041 ccatccacca atcaatttct tgaacggccc atccaccaga ttcataagaa tactttggtt
    23101 actggtcaac tggttgcggg tagtaaacgt tttgctgatc ccagccttag agacatctac
    23161 cgccgcagtc atctgcccag gagtggactc cagaatccga cttccggtac aacccggcaa
    23221 aaactgagga taagactgaa cgtcattcac taactgatac atttgctccg cgctgtaggg
    23281 taccagtgcg gtccggctaa tctgaggcat aacaatttcc atcaacaaaa atccaacaaa
    23341 taatatcatt tatcctgcag taaaaaaaac gctatcccgg cgctgggtaa catccggttc
    23401 atgctaagat agagccttgt cccccgcagg attgatatgg ggtattttcg atttcagatt
    23461 accgatgatt cacgacgctt atgacgaaga aaaaagcaca taaacctggt tcagcgacca
    23521 tcgcgcttaa caagcgcgcc cgtcacgaat actttattga agaagagttc gaagcgggac
    23581 ttgccctgca aggctgggaa gttaaatccc tgcgcgcagg aaaagccaat attagcgaca
    23641 gctatgtcct tctgcgcgac ggagaagcgt tcctgtttgg cgcaaacatc acgccaatgg
    23701 ctgtggcttc tacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta cttctcaacc
    23761 agcgcgaact tgattcgttg tacggtcgcg tcaatcgaga aggctatacc gtagtggcgc
    23821 tctccctgta ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc aaaggtaaga
    23881 aacagcacga taaacgttca gatatcaaag aacgcgaatg gcaggtggat aaagcgcgta
    23941 tcatgaaaaa cgcccaccgt taaacctgca ctccaattct tgacctgttc ctcaccgcgc
    24001 ctccctctcc ggcggcgcga atgaacatct tattggctat cacatccgac aaaaatgttg
    24061 ccatcccatt gcttaatcga ataaaaatca ggctacatgg gtgctaaatc tttaacgata
    24121 acgccattga ggctggtcat agcgctcata aatctggtat acttgccgtt acacattggg
    24181 gctgattctg gattcgacgg gatttgcgaa acccaaggtg catgccgagg ggcggttggc
    24241 ctcgtaaaaa gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta gcagcttaat
    24301 aacctgctta gagccctctc tccctagcct ccgctcttag gacggggatc aagagaggtc
    24361 aaacccaaaa gagatcgcgt ggaagccctg cctggggttg aagcgttaaa acttaatcag
    24421 gctagtttgt tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag actgactaag
    24481 catgtagtac cgaggacgta ggaatttcgg acgcgggttc aactcccgcc agctccacca
    24541 ctttaagaag gactacaacc ggacagtagc aataaataca gccacttacg gacacaagcc
    24601 agacagtagt cggactgaaa aaagacaaaa atatacacgt gaaatgcacg tgcacttcta
    24661 aaaaacccca gttatcaccg actggggttt ttcaatttgt aactaagggt aacaaccccc
    24721 ccgttttttt catctcactc accttgatac tgtttatttg tacagttaaa aataatactg
    24781 tatacaaaca cagtatagag ggacttttat gcgtattgag atctgcatag ccaaagaaaa
    24841 aatgactaaa atgccaaacg gtgctgtgga tgcgttaaag gaagaattaa cccgacgcat
    24901 cagtaaacgt tatgacgatg tagaggtgat cgtaaaagcc accagcaacg atggcctttc
    24961 tgttacgcgc accgccgata aagattcagc taaaactttt gttcaggaga ctctgaaaga
    25021 tacctggaag tctgctgacg agtggtttgt tcactaatta acacgtaaaa tcggtaacgg
    25081 ctggaaatca ttcaatactc gcactatcga aagttcacca gccaaccgca gcacgtcctg
    25141 catacgtcgt gtctgcggtt tttctttttc gcttacattg tgtctggttc ttccggccac
    25201 tcaatatcag gtgcagttga tgtatcaaca cggttcagca acacccgata ctttttctag
    25261 gcttccagca atgaggtttc ttcctccgtt gcaatttcca gatctgcagc atcctgaagt
    25321 ggggcgcacc acgcaaacat cacaaaatat tgtcgctgcg atgaacctgt acaataaata
    25381 atgaatcacg gtacgtttct gtgtataacg tactgtagtt ttctttacga tttatagctg
    25441 tactggtgag ccatgagtaa tctgaatcca tgcatggcgt gtggtgccgt tgtgcatttt
    25501 ctgcgtctct tttactggtc cgaagccgat gatgctgaag cagtcctctc ctctagcctc
    25561 atccctgtat agcacgcatc aaataatctg ggatatcatt tcgtagtttt acaatatcaa
    25621 tatccgaatc gatatgtttc tggtaaaact tgtccgcaag caagtttgct atggtatctg
    25681 atgtacgatt ttttacatcc cggctcatac catcacaaag agcatccatt ttcttgccaa
    25741 aagaagcact ccccattatt acatgattaa tagaactttg tgctgcctta acatcaaggg
    25801 ttagttgatt ctcctggctc acaagaaaaa agactctttg cctctcttca gtggaaattt
    25861 ttgcattttt ttgaaaacag taattatcaa tctcctggtt gatcaccctt gagggatata
    25921 ctatccgttc atcttttatc cactcatcaa tatctttagt actctgcctt ctcaccgttt
    25981 ccagtatatt gccgctaaat ttgcgaacag aaaagctaat agtccgttct ccaattgaac
    26041 attttacgac ctcatcattc ttccctcgga aagagttcct gaaggagttc tctctatggc
    26101 aacttatacc aaaagagctg aaagacatac ctgtagtatt catcggaatt ataattcctt
    26161 tttataaaaa agtgtttaca atattgcata cttgcgttta ttgtagccag aaaaaacatg
    26221 catgcaataa tattctatta aaaattgtat tgttttattc acatatcatg agcggcatgt
    26281 tgattcttgt taccaatata gttcacaata tggattaaaa aggataataa tatgccagtc
    26341 atattaaatt tttctaatgg aagtgtattg ccggaaaatg agctggaggc tttacgacat
    26401 atagcaagaa gcaaccagaa tgacactatt actataggag gccgtaatat gaggcttcat
    26461 tatatccagt ttatggatgg ttttagtgtt gagcctattc ttggtggact ttgggaccat
    26521 cttggggcaa gagaggctca tcatctcgca gaccgcctga caagacaact taacggaggt
    26581 aacacttttc tgcaggcata cagcttatat ctggagcaga ggcaagctgc cccgcttgtg
    26641 caggagagcg tcataaaaac actactagat cgaataaatt cgaatgcatt tcccgttagt
    26701 ttacaagact tttcctgtac tgaggaacat cttaattgtc cgataacgct acatattcct
    26761 gagacaggtg tttttgtcag aaatgctcga aattcagaaa tatgtgcatt atatgatcag
    26821 gaagcattga ctgaacttat cctgcgtaac gctctccacc ccctcagccg tgaccccttt
    26881 gctccagaaa tgattataag caaagacaag tgtcatttta atataacaaa acaatgtttt
    26941 tacgcattac ccatataccc acttcaacaa aacagtattt aaaaataaaa gcacaatata
    27001 agaagcatat tttcaataag ttaatcacca tataacaaac atatagcgag gagactattt
    27061 atgcccaaaa tatcatcagt tgtatcatca tgttaccatc tgttcagtga acatcaacaa
    27121 ctttcaaatg aaacaacaat gacgaacccc gtctccagaa gaattgttca taaagaatat
    27181 ggtatatctt taaaatccgt tcctgtatgg ttggctacag ctaaaactcc ccttgctcta
    27241 ctcaatggca gacatacaag aagtcactca tttattattg cagggacccc aggaatggga
    27301 agcaggagcg gagcccaata ctatgccata aacagtgatg ataaacgctc ccgaatagac
    27361 attgactctt tatttttaaa aaagttaaat aatgtgcgaa atcaaaataa atttccaatc
    27421 gatgtaaaag aaacggttat aaaactacaa gggcaaaaat tcacatgcat tgaagatttt
    27481 tataaaaggt ataatgaaac caggttaaag gccaatacca atatccaaca agaacaaatt
    27541 gcagatgaag taaaatcact tacatatttg attccttcag aaaaaaaaga gatgtggata
    27601 tataaaaaca atggaaaaga taatgcaaaa ccaaacttag gagagcgaga cgtaagaatg
    27661 ttcgaaaata ttagttctga tgatacagat aagataacag gaaggaaatt ttcagagtta
    27721 ggtgagtatc tttattcagg gaatgtaata aaactcagtc agttatcaat tcgttactta
    27781 cccaatatca gctcaatctc attaatagag acaaaacaga gtttgttgct acatcgattg
    27841 tattcagatg aagtacttca gagaaatgga acgcttatcc cgacaccact acatgaagaa
    27901 aaatcaattc cagctgacaa tataaaaaca atgctcaaca acataccgac ttacaaaatg
    27961 ttaccgccat tcacagaaac acaaggtaat tgttcttctg gcgcagccac gtttttacgc
    28021 aaatcaggcg ccgaagaaaa agatattctt gcatgtagcc cccgaaatta tgggctgcat
    28081 cataacataa aaacatggga ccccttggtt agaaattaag gatccaaata ttatatattt
    28141 catataaagc aagaaaaaaa tattgtctga ataattgttc agacaatata tccttacatg
    28201 gcactattat aataaactat taatataaaa acacaccaac aaaaaaaatt aagcatcact
    28261 tgcaacaaag gattcttttt ttg
//
LOCUS       DABSYT020000056        28198 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.056, whole genome shotgun sequence.
ACCESSION   DABSYT020000056 DABSYT020000000
VERSION     DABSYT020000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.056"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(76..1341)
                     /locus_tag="ID725_RS14505"
                     /old_locus_tag="pgaptmp_002901"
     CDS             complement(76..1341)
                     /locus_tag="ID725_RS14505"
                     /old_locus_tag="pgaptmp_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7851446.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="ID725_RS14510"
                     /old_locus_tag="pgaptmp_002902"
     tRNA            complement(1539..1614)
                     /locus_tag="ID725_RS14510"
                     /old_locus_tag="pgaptmp_002902"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ID725_RS14515"
                     /old_locus_tag="pgaptmp_002903"
     CDS             complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ID725_RS14515"
                     /old_locus_tag="pgaptmp_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGV7851447.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2580..2663
                     /locus_tag="ID725_RS14520"
                     /old_locus_tag="pgaptmp_002904"
     CDS             2580..2663
                     /locus_tag="ID725_RS14520"
                     /old_locus_tag="pgaptmp_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851448.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2825..4960
                     /gene="speC"
                     /locus_tag="ID725_RS14525"
                     /old_locus_tag="pgaptmp_002905"
     CDS             2825..4960
                     /gene="speC"
                     /locus_tag="ID725_RS14525"
                     /old_locus_tag="pgaptmp_002905"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGV7851449.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ID725_RS14530"
                     /old_locus_tag="pgaptmp_002906"
     CDS             complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ID725_RS14530"
                     /old_locus_tag="pgaptmp_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGV7851450.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ID725_RS14535"
                     /old_locus_tag="pgaptmp_002907"
     CDS             complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ID725_RS14535"
                     /old_locus_tag="pgaptmp_002907"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGV7851451.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ID725_RS14540"
                     /old_locus_tag="pgaptmp_002908"
     CDS             complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ID725_RS14540"
                     /old_locus_tag="pgaptmp_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGV7851452.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ID725_RS14545"
                     /old_locus_tag="pgaptmp_002909"
     CDS             complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ID725_RS14545"
                     /old_locus_tag="pgaptmp_002909"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGV7851453.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9128..9847
                     /gene="trmB"
                     /locus_tag="ID725_RS14550"
                     /old_locus_tag="pgaptmp_002910"
     CDS             9128..9847
                     /gene="trmB"
                     /locus_tag="ID725_RS14550"
                     /old_locus_tag="pgaptmp_002910"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGV7851454.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9847..10173
                     /gene="yggL"
                     /locus_tag="ID725_RS14555"
                     /old_locus_tag="pgaptmp_002911"
     CDS             9847..10173
                     /gene="yggL"
                     /locus_tag="ID725_RS14555"
                     /old_locus_tag="pgaptmp_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGV7851455.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10357..11076
                     /gene="yggN"
                     /locus_tag="ID725_RS14560"
                     /old_locus_tag="pgaptmp_002912"
     CDS             10357..11076
                     /gene="yggN"
                     /locus_tag="ID725_RS14560"
                     /old_locus_tag="pgaptmp_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGV7851456.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11252..12298
                     /gene="ansB"
                     /locus_tag="ID725_RS14565"
                     /old_locus_tag="pgaptmp_002913"
     CDS             11252..12298
                     /gene="ansB"
                     /locus_tag="ID725_RS14565"
                     /old_locus_tag="pgaptmp_002913"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGV7851457.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12415..13422
                     /gene="yggM"
                     /locus_tag="ID725_RS14570"
                     /old_locus_tag="pgaptmp_002914"
     CDS             12415..13422
                     /gene="yggM"
                     /locus_tag="ID725_RS14570"
                     /old_locus_tag="pgaptmp_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGV7851458.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     IQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="ID725_RS14575"
                     /old_locus_tag="pgaptmp_002915"
     CDS             complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="ID725_RS14575"
                     /old_locus_tag="pgaptmp_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGV7851459.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="ID725_RS14580"
                     /old_locus_tag="pgaptmp_002916"
     CDS             complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="ID725_RS14580"
                     /old_locus_tag="pgaptmp_002916"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HGV7851460.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSACYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="ID725_RS14585"
                     /old_locus_tag="pgaptmp_002917"
     CDS             complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="ID725_RS14585"
                     /old_locus_tag="pgaptmp_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGV7851461.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="ID725_RS14590"
                     /old_locus_tag="pgaptmp_002918"
     CDS             complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="ID725_RS14590"
                     /old_locus_tag="pgaptmp_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGV7851462.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="ID725_RS14595"
                     /old_locus_tag="pgaptmp_002919"
     CDS             complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="ID725_RS14595"
                     /old_locus_tag="pgaptmp_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGV7851463.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16988..17968
                     /gene="yggR"
                     /locus_tag="ID725_RS14600"
                     /old_locus_tag="pgaptmp_002920"
     CDS             16988..17968
                     /gene="yggR"
                     /locus_tag="ID725_RS14600"
                     /old_locus_tag="pgaptmp_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGV7851464.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="ID725_RS14605"
                     /old_locus_tag="pgaptmp_002921"
     CDS             complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="ID725_RS14605"
                     /old_locus_tag="pgaptmp_002921"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGV7851465.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="ID725_RS14610"
                     /old_locus_tag="pgaptmp_002922"
     CDS             complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="ID725_RS14610"
                     /old_locus_tag="pgaptmp_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGV7851466.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="ID725_RS14615"
                     /old_locus_tag="pgaptmp_002923"
     CDS             complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="ID725_RS14615"
                     /old_locus_tag="pgaptmp_002923"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGV7851467.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="ID725_RS14620"
                     /old_locus_tag="pgaptmp_002924"
     CDS             complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="ID725_RS14620"
                     /old_locus_tag="pgaptmp_002924"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGV7851468.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21021..21728)
                     /gene="endA"
                     /locus_tag="ID725_RS14625"
                     /old_locus_tag="pgaptmp_002925"
     CDS             complement(21021..21728)
                     /gene="endA"
                     /locus_tag="ID725_RS14625"
                     /old_locus_tag="pgaptmp_002925"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGV7851469.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="ID725_RS14630"
                     /old_locus_tag="pgaptmp_002926"
     CDS             complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="ID725_RS14630"
                     /old_locus_tag="pgaptmp_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGV7851470.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22397..23791)
                     /gene="galP"
                     /locus_tag="ID725_RS14635"
                     /old_locus_tag="pgaptmp_002927"
     CDS             complement(22397..23791)
                     /gene="galP"
                     /locus_tag="ID725_RS14635"
                     /old_locus_tag="pgaptmp_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGV7851471.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(24228..25382)
                     /gene="metK"
                     /locus_tag="ID725_RS14640"
                     /old_locus_tag="pgaptmp_002928"
     CDS             complement(24228..25382)
                     /gene="metK"
                     /locus_tag="ID725_RS14640"
                     /old_locus_tag="pgaptmp_002928"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGV7851472.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            25438..25689
                     /gene="yqgD"
                     /locus_tag="ID725_RS14645"
                     /old_locus_tag="pgaptmp_002929"
     CDS             25438..25689
                     /gene="yqgD"
                     /locus_tag="ID725_RS14645"
                     /old_locus_tag="pgaptmp_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGV7851473.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            25800..25943
                     /gene="yqgG"
                     /locus_tag="ID725_RS14650"
                     /old_locus_tag="pgaptmp_002930"
     CDS             25800..25943
                     /gene="yqgG"
                     /locus_tag="ID725_RS14650"
                     /old_locus_tag="pgaptmp_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGV7851474.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            26037..26168
                     /gene="yqgB"
                     /locus_tag="ID725_RS14655"
                     /old_locus_tag="pgaptmp_002931"
     CDS             26037..26168
                     /gene="yqgB"
                     /locus_tag="ID725_RS14655"
                     /old_locus_tag="pgaptmp_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGV7851475.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            26177..28153
                     /gene="speA"
                     /locus_tag="ID725_RS14660"
                     /old_locus_tag="pgaptmp_002932"
     CDS             26177..28153
                     /gene="speA"
                     /locus_tag="ID725_RS14660"
                     /old_locus_tag="pgaptmp_002932"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGV7851476.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
BASE COUNT         6897 a         7035 c         7418 g         6848 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggtcca
     1741 ggcggcggaa agcggcattg ccagaaggag tgctggaagc atgttgacca ccgggaacat
     1801 tttaatgcca cagatgcgta atccggttgc cagcagcaat aaaccgccta cggcgctgaa
     1861 gtctgccatc atcgacggtg tggtcagcgg taatatcagc gcggcagccc acgccagcgt
     1921 taactggatg atcagtaatg ggatactaat caccgatacc gcaataccca gcgagcaggc
     1981 gaagatcatc gccgtaaaga aatcaagaaa tgacttggcg attaaaatac tcggatcgcc
     2041 ggtcatccct tcgttcatcg ccccgaagat cccggtgccg ctggcgcaaa acaggacaat
     2101 aatcgcgaca taattctgaa taaaagattc atgcgctggc ttcttacgtg agtgacgaaa
     2161 cagattttgt gctttggcga ccgctgtatt gacgcctttc tccagcagac aaatttcgcc
     2221 gattaatgct ccgagtaagg ttgctaaaac catcgccgga aggttggcgc atttcaccac
     2281 cagtaaaata ccaatcccca gcgatgccaa accaaaaatt gatgtcatgg agacgcggat
     2341 acgttccggt aagcgttggc taagcagtgc accgagcatg ccacccagta atattgcact
     2401 tgcgttgata aaagggccga tgaccacaag agttcctgtt aactgtattg tttgattttc
     2461 ttattatgaa ctttttgcct tgttgatggc ttgtctggtc gcaaagtcct ggcttgcacg
     2521 ccttagcgaa aggtgccatg attgcgcgaa ttttctcctc tctgtacgga gtttgcccga
     2581 tgcacgccat ctccttacat tctctcgctt atcgccgttt cgcgcgaaac ccttctcttt
     2641 ttatgctact gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc
     2701 agagcctttc tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata
     2761 tctcatgggg cagggtttta caccttgtcc agtattctta cttccccgaa acgggtttgc
     2821 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     2881 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     2941 cgctgcggat agtcgcagtg gcattcttac gttgcttaag cgcaccggtt ttcatctacc
     3001 ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg cggtaatcaa
     3061 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     3121 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc
     3181 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     3241 cgatttcttt ggtgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     3301 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaattcgcag ccaaagtctt
     3361 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     3421 gaatgcgctg ttaacgcgtg gcgatctggt gctctttgac cgtaacaacc ataagtcgaa
     3481 tcatcacggc gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgtaa
     3541 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     3601 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     3661 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     3721 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttatg aacaatttat
     3781 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     3841 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     3901 taaaaaagat aaccatatcc gcggacaggc gcgtttttgc ccgcataagc ggttgaataa
     3961 cgcctttatg ctccatgctt ctaccagccc tttctatccg ctgtttgctg cactggatgt
     4021 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     4081 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     4141 gcccgttgtt gatggcaaat tgtggcagga ttatccgaca tcagtgttag ccagcgaccg
     4201 tcgttttttc agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga
     4261 tcagtatttt gttgatccgt gcaagctgtt actcactaca ccaggtatcg atgccgaaac
     4321 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactatc tgcgtgagaa
     4381 cggcattgtg ccggagaagt gcgatctcaa ttccattctg ttcttattaa ctccggcgga
     4441 aagccacgag aagctggcac aactggtggc gatgctggct caatttgaac agcatattga
     4501 ggatgactcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     4561 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga
     4621 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     4681 cccccaggat gcgcatagcg cttatattcg cggtgacgtg gagttggtgc ggattcgtga
     4741 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     4801 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcac tggaagaagg
     4861 ggtgaatttg ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc
     4921 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa aacgggtcgc
     4981 cttctggcga cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg
     5041 acacgaacgt gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa
     5101 ccagcgaaaa tcaaccatac ggtctgccag tcggtaatac cgttttgggt gtacatctca
     5161 acaactttac cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg
     5221 aacatccctt gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa
     5281 ccagagatgt tgaagaagtc gaacgcacag ccgtaaacaa tcatcgatag aaccagcagt
     5341 acggtgccga acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc
     5401 acaatactga tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc
     5461 agaatgaaca gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcaca
     5521 ataaagctgc tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac
     5581 atgttggtaa tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata
     5641 cgcttgtttt taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc
     5701 gcttgctgtt tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg
     5761 gaaagtgctg cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc
     5821 acccacattg ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta
     5881 acgatatcca tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc
     5941 gttggcatat aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtcgtg
     6001 acctgtgccg ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat
     6061 ttcgcactta accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg
     6121 atacccagtg agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac
     6181 atataggaac cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg
     6241 attttcagct gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt
     6301 ttgaaaaagc acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt
     6361 ttttgttacc tgtggcaaat aattgcaaac attcctaatt tgcaaaacga aattgtgata
     6421 tggataacat ttttgcccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg
     6481 taggattttt gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg
     6541 cgggtcgtca gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attagccgcc
     6601 tgaatcttat cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg
     6661 atgacggcat aacgccggga tgtagggtta tcaattccgc cgagataaac attgttcagc
     6721 atcgccagat acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg
     6781 ctcggcgtgc cggatttccc ctgcgagcgg aacacatctt tcccggcagt atgttgtacc
     6841 acctgcatta accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca
     6901 gtctgcataa ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg
     6961 accatgccga gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg
     7021 acgctgtaga tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa
     7081 tccgcgaagt tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc
     7141 tgaccgtaaa ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg
     7201 accgaactcg gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg
     7261 gcaggttctg tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg
     7321 ctgcgggtct gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc
     7381 tctttgaatc cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa
     7441 ccgttggtgt ctttgaccca ggcttcgtta taggtaccgc cttttttggt cgtcgaacag
     7501 gagatgagca acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg
     7561 tgttgtgttt gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt
     7621 atcttccggc gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat
     7681 ctcctgctcc agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat
     7741 cagcatggtt tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat
     7801 gcgttttccc agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag
     7861 gaaagtacaa aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac
     7921 cggcgcgcca gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga
     7981 cggcggttgc gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga
     8041 cgacacggga agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa
     8101 cgcggtcagc tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa
     8161 actttcttcg tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg
     8221 acgctgcgcc agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc
     8281 cggcagcgtc tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc
     8341 aatacatccg ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat
     8401 cgcgcccaaa tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg
     8461 ctcgcttaaa ctccataatt tattctcgac ctcttttttc ccaggccagc cgcttacagc
     8521 atagcagcgc gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag
     8581 agaaagcgat agaatcgcgc ctgcggtgga acgcccgacg cccggcagtg ctgcaacttc
     8641 ctcaaaggtt tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgctttatg
     8701 cagattgcgc gcgcgggcgt aatagccaag ccccgtccac aagtggagaa cttcgtcgag
     8761 cggcgcattg gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg
     8821 gataacggtc gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg
     8881 cgtcttgtca atttgccagg gcagagtttt tcgcccgtat ttatcgtacc agtccagaac
     8941 ctgggctgaa aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat
     9001 tgcagcacag cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc
     9061 ttgcatccgg caactaactt tggataatgc ccgttttcag aacactttca caagcgacta
     9121 aatctttatg aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg
     9181 ccgtatccgt agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct
     9241 ggaaaactac tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc
     9301 gctttttggc cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct
     9361 ggtggcaatg gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc
     9421 gggcgttggt gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat
     9481 gtgtcacgat gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt
     9541 gcagctcttt ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca
     9601 ggtgccgttt gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc
     9661 gaccgactgg gaaccttatg cggaacatat gattgaagtg atgtcttcta ttgacggtta
     9721 taaaaacctg tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa
     9781 atttgaacaa cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt
     9841 gaaataatgg caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc
     9901 caggaattag gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt
     9961 gataaaaccg ttgatgattt tattaacgag gttatcgaac cgaacaaact ggcctttgac
    10021 ggcagcggtt atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc
    10081 gaagaacatc aggcgattgt gcgtaagtgg ctggaagagc gcaacctgga tgaggtacgc
    10141 accagcgaac ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca
    10201 aaactgcctg atgcgctacg cttatcatgc ctacaaagat gcacgatcga gtaggcggga
    10261 taaggcgttt acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca
    10321 gcaaatgctg gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg
    10381 gcagcacttt cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg
    10441 cgtgacgatg tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg
    10501 gtgatcacgc cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc
    10561 cagcgcgagc aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat
    10621 gaaggcgcga aaagccgcgt cgagaaagcc cgtattgctc tggataaaat tatcgttcag
    10681 gagatgggcg aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa
    10741 gagcagatga accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc
    10801 attgatcagg ttcgcgctga aggccagcaa ttagtgaatc aggcaatggg cggaatttta
    10861 caggacagca ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta
    10921 cagaatgtgc tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag
    10981 caggaaaaag atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa
    11041 gatagccgca aagccctggt cgggaattta aaataatcct ctattttaag acggcataat
    11101 acttttttat gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca
    11161 aaatatattc acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa
    11221 tgcgtagcgt tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc
    11281 cgcactggtt atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc
    11341 aaccggcggg accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg
    11401 taaagttggc gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt
    11461 taaaggcgag caggtagtga atatcggctc ccaggacatg aacgatgatg tctggctgac
    11521 actggcgaaa aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttactcacgg
    11581 taccgacacg atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc
    11641 ggtggtgatg gtcggcgcaa tgcgtccgtc cacgtctatg agcgcagacg gtccattcaa
    11701 cctgtataac gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt
    11761 ggtgatgaac gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt
    11821 agcgaccttc aagtctgtta actacggtcc gctgggatac attcacaacg gtaagattga
    11881 ctaccaacgt accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct
    11941 gaatgaactg ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc
    12001 taaagccctg gtagatgcgg gctatgatgg catcgtgagc gctggtgtgg gtaacggcaa
    12061 cctgtataaa tccgtgtttg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt
    12121 gcgttcttcc cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa
    12181 atacggtttt attgcctctg gtacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcaact
    12241 ggctctgacg caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc
    12301 atttcgcccc ggtatcgcgc cggggctttt tcacttcaga cttacgtcca ttgccaattt
    12361 taattgccct aatgataatc accagaataa attattccgc gcgagggttt tcgggtgaaa
    12421 aagcaatgga ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat
    12481 ggcgaaccgc caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt
    12541 ctcaagcttg atgccatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg
    12601 tcagcggaag gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca
    12661 gattacgaac tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg
    12721 ttaaccagta aaggaacgcc cgcgtctggc tggacggtga acttttactc ttttcaggcg
    12781 gcagctagcg atcgtggtcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc
    12841 gtgaatagcg aagatttcaa ttatcgcttt agtcagcttg agactgcgtt gaatatccag
    12901 aagaactcga ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg
    12961 gcgcagaaag cagcggatgc atattgggga aaagatgcaa atggtaagca aatgacccgc
    13021 gaagatgcct ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc
    13081 gaggcgttcg cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat
    13141 aaacagagtg aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac
    13201 gaacaacggc gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac
    13261 tggataacgc tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt
    13321 aaaaaagtcg cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa
    13381 aaagataccg aacaactgcg gcgcaatggg gtgattaagt aagttttgat atagcgcacg
    13441 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgcta
    13501 gcgcgtctta tcagacctac aacagctgcc acatgtcggc tgaggtgctc acgccgcatc
    13561 cggcaatcaa cgtctgatgc gacgcttgcg cgtcttatca gacctacaac cgttgccaca
    13621 tgtaggctgg ataaggcgtt cacgccgcat ccggcaatac aagtttactc agccagaaaa
    13681 agctccagca gcgaatttaa aaacagcttc ccatgttccg taatctgcca gtaatccgca
    13741 cattcggtga gataaccctg ggcaatcgcc tcgtctaact gtgggcgaat cacatcttcg
    13801 caaagcccgg tatacgcact aaactccacg cgcggcgcag gttccagcaa ccggaagcga
    13861 ttcataaaga actcaaacgg cttatctgcg gcttcgacat cacgctggct ttccagatac
    13921 cttccttgca taaaaccacg cggatgacgc gttttggtgg tacgcagaat gcgcccatcc
    13981 gggaaggtca ctttgccgtg cgcgccgcag ccaataccga tgtagtcacc aaagcgccag
    14041 tagttgagat tgtgctggca ctgataaccg ggtttggcgt aagcggaagt ttcatattgc
    14101 tgataacccg ctgcggttaa taactgatgc ccctgttcga aaatatccca cagcgcgtcg
    14161 tcgtccggca gtaccggcgg gcgtgaacca aacagcgtat taggttcgat ggtcagttga
    14221 taccaggaaa gatgcggcgg attcagttca atagcctggc gcagatcgcc aagcgcctct
    14281 tccagcgatt gatccggtag cccatgcatc aaatcaaggt taaagctacg taaccctaaa
    14341 ccgctcgcca gctttgccgc tcgtttcgct tcttgcgggc catgaatacg tcccagccgt
    14401 ttcagctttt cttcgctaaa actctgtacc ccaatagaga tacgatttac accggcacgc
    14461 tgataatcaa caaagcgatc ggcttctacc gtaccggggt tcgcttccat ggtaatttct
    14521 gcatccgctg ccagcggcaa acgcgcacgc acgccgtcca gcagcgtttg catcgccgag
    14581 ccggaaagca ggctcggcgt accaccgcca ataaagattg tctttacttc acggccctga
    14641 gcgtaagcca catcgttgtc cagatcgtta agcagatgct gaacgtaatc gtcgtgcggc
    14701 acttctcctt tcaacgcgtg agagttgaaa tcgcagtacg ggcatttctg cacgcaccac
    14761 gggatgtgaa tgtagagact cagcggcggt aatttaacca ttacgtaaag cgtccagcag
    14821 cagtttcaat gcctgaccac ggtgggaaat ggcgctcttt tcttcgcggg tcagttcggc
    14881 agcggttttc ccttcggaag gtacgaagaa gattggatca taaccaaagc caccagtgcc
    14941 cgccggttcg cgagtaatca cacccggcca gctgccatgg cacaccagcg gggtcggatc
    15001 ttctgcgtga cgcagataca ccagcacgca gtggaaacgc gcctgacgtt ggtcgtccgg
    15061 tacgtctttc agtgtttcca gcagtttttg cagattcttt tgatcggtcg cgtcttcacc
    15121 ggaataacat gcggagtaaa tacccggcgc gccgccaagc acatctaccg ccaggccaga
    15181 gtcgtcggca attgccggta aaccggtcac ttttgccgcg tggcgcgctt tcagaatcgc
    15241 gttttcgata aaggtcaggc cggtttcttc agcggaatca acgccgagtt ctgtttgggc
    15301 cacgatatca agaccgaagt cgctaagcag cgacgccagc tcacgcactt taccggcatt
    15361 gccggttgcg aggacaactt tttgcatagg atacctaatt aattaacgcc gcgacttctg
    15421 gcgggatttg ttgcggatta atgattttaa tttgtttgtg gcggccaagt tcgccttttt
    15481 caatcaccac ctggcttttg gcaacccgga attgcttacc gagaaacttc accagatggc
    15541 tgttggcctg accgtcaacc ggcggcgcgg taatggcgac ttttacctcg tcgccatgta
    15601 aaccgacaat agaatcacgg ctggctttcg gctgaatata gagccgtaaa accagaccgt
    15661 catcgttaac tgttacggca ctcataacgc catccacagc cccggcagca gcatatttcc
    15721 ggtcgcctgt aatacttctg cgacacccat attgatgaca tacagcagca gaacgaggat
    15781 catcggcgag aaatcaatcc cccccattgc cggtagcaag cggcgaatcg ggcgcagcag
    15841 cggatcggcc agctgaatca gcacgtattc aatcgggcta cggccctggc ttacccagct
    15901 cataatcgcc atcaccagca ggacccagaa aatcagcagg ccgatggttt tcagcagaat
    15961 cagtaaaccg gcaatccaga tgattggcag gaaggtcacc actttaaaca gcacgatggc
    16021 tttgataaaa ctgagaatat aggcaaccag cagcgaggcg ctatcaatgg gtcccattgc
    16081 cggaataacg cggcgcagtg gcccgataat tggctgcgtt accttcacta caaactgtga
    16141 gaaggggttg taaaaatcac aatgagccca ctgcatccag atgcgtaata acagcaccat
    16201 ggtatacagc tcaatgaccg ttgaaagcag gaaagtcaac gtattcatgg cgttccttta
    16261 attccttatt ttttagagta atcacgcgca ccaaaaattg cagtgccgat acgaaccatc
    16321 gtgctacctg ccgcaatggc ggcttccata tcgtccgaca ttcccagaga gagcgtgtcg
    16381 atatgcgggt agcgcgtttt cagtccggca aatgctacag ccatttggcg tgcaacttca
    16441 aactgcctta catattctga ctcaggcgca gggattgcca tcagcccgcg cagacgtaaa
    16501 cgcggtagtt cagcgaccgc agctgccagc tcgtccagtt ctgccagttg aatcccggac
    16561 ttactgtttt catcactaat attaatttga atcagtacgt taagaggggg aagttctgcc
    16621 gggcgctgat cgttgaggcg ggtagcaatg cgcaaacggt cgatggtatg acaccagtcg
    16681 aaatgctctg ccaccaggcg gcttttatta gactgcaacg ggccaataaa atgccattct
    16741 aatcctgtta cgcccagttc ctgaaagtgg cgaattttat ctaccccttc ctgaacgtag
    16801 ttttcaccaa attgacgctg cccggcatca atggcttctg cgatggcgct cgcaggtttt
    16861 gttttactga ctgcaagcag cgtaatttct tctggagaac ggccgcaacg cgttgcagcc
    16921 gctgagattt tgtcccggac ctgtgccagg ttatgcgcaa tatcgttcat tttccgagga
    16981 tcttagtatg aatatggaag aaattgtggc ccttagtgta aagcataacg tctcggatct
    17041 acacctgtgc agcgcctggc ccgcacgatg gcgcattcgc gggctaatgg aagctgcgcc
    17101 gtttgatgcg ccggacgtcg aagagctact gcgggagtgg ctggatgacg atcagcgggc
    17161 aatattgctg gagaatggtc agctggattt tgccgtgtcg ctgacggaaa accagcgatt
    17221 gcgtggcagc gcattcgcgc aacggcatgg catttcgctg gcgttacggc tgttaccttc
    17281 gcactgcccg cagctcgaac agcttggcac accaccgata ttgccggagt tactcaagag
    17341 cgagaatggc ttgattctgg tgacgggggc gacggggagt ggcaaatcta ccacgctggc
    17401 ggcgatggtt ggttatctca atcaacatgc tgatgcgcat attctgacgc tggaagatcc
    17461 tgtggaatat ctctatgcca gccagcgatg tttgatccag cagcgggaaa tcggtttgca
    17521 ttgtatgacg ttcgcatcgg gattgcgggc cgcattgcgg gaagatcctg atgtgatttt
    17581 gctcggagag ctgcgtgaca gcgagacaat ccgtctggcg ctgacggcgg cagaaaccgg
    17641 gcatctggtg ctggcaacct tacatacacg tggtgccgcg caggcagttg agcgacttgt
    17701 ggattcattt ccggcgcagg aaaaagaccc cgtgcgtaat caactggcag gtagtttacg
    17761 ggcagtgctg tcacaaaagc tggaagtgga taaacaggaa ggacgcgtgg cgctatttga
    17821 attgctgatt aacacacctg cggtggggaa tttgattcgc gaagggaaaa cccaccagtt
    17881 accgcatgtt attcaaactg ggcaacaggt ggggatgata acgtttcagc agagttatca
    17941 gcagcgggtg ggggaagggc gtttgtgacg ttgccatgcc cgatgcgcaa gcttatcggg
    18001 cctacaaagg caataaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgc
    18061 tgccacatgt aggccggata aggcgttcac gccgcatccg gcaataaggc gtaaggcgcg
    18121 atgatattat ttacactcca gcaggcgatt aaatctcctt aatatccctg ctcgaaatag
    18181 ctttcgagaa taataaccgc agaggcagag tcgattttgc ctttgttgag cgcccgatag
    18241 ccgccctgtt caaacagacc ggaacgtgcc tccacggtgc taagacgctc gtcatggagc
    18301 tttacttcaa caccgaaacg accatgaata cggttagcaa atttacgcgc tctggcggtt
    18361 aacggctgtt cggtgccgtc catattcagc ggtaaaccga cgatgatttc gtctggctgc
    18421 cactctttca gtaaacgttc gatgaggttc cagtccggcg taccgtcctg tgctttaatt
    18481 gcaggcaaag ggcgagcggt gccggtaatg cgttggccga ccgctacgcc aatgcttttg
    18541 gtgccgaagt cgaaggcgag taaggttcca ctcatcaggc gtgtcctgcc acaccaggca
    18601 tggtgagaat atccacacca atcagttttg ccgcctcgcg ccagcggtcg gcaattggcg
    18661 ttttgaacag aatattcaga tctgccgggg cggttagcca cgcgttatcg agaatttctt
    18721 gttccagttg acctttttcc caggaggcat agcccagagc caccaatacg tcagacggtt
    18781 gtttatcggt gccgagcgtt tccagcacat cgcgggaagt ggtcatcact gtgttgtctg
    18841 aaatgcgaat gctggaagca aaattggagg gcggagtatg caaaataaac ccgcgatctt
    18901 cagccagtgg gccgccgagc ataaccggtt tatccagacg gattgattca tcacgcggct
    18961 ccggcgtgat cttcagcttt tccagaatcc cttcaatttt gagattttcc agcggcttgt
    19021 tgacgatgat ccccattgca ccattggtat tatgttcgca aatgtagacc acggaacgac
    19081 ggaaaatcgg atcctggaga gcaggcatgg caataagaaa gtgatgctgt aaattcattg
    19141 tcagaggttc tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt ggaaagaagg
    19201 gcgctgtcac tcagagtctc aacgagatcc ttctcgctaa ggtgggttac tactgctgta
    19261 aacgtgcttc gatggcatcc attaacattc cggtgatcga caccggaaac tctgcttcaa
    19321 tctcacgaat acaggttggg ctggtgacgt taatttcagt cagacggtcg ccgatgatat
    19381 ccagaccaac aaaaatcagc cctttttctt tcagcgtcgg cccgatctga cgggcgattt
    19441 tccagtcact ttccgtcagc ggacgaggtt caccgcgacc accggcagcc agattgccac
    19501 gggtttcgcc cccctgcgga atacgcgcca ggcagtacgg tactggttcg ccgtccacca
    19561 ccagcacgcg tttgtcgcca tctttaatgg ctggcagata attttgtgcc atgcagtagc
    19621 gagtgccgtg ttcggtcaag gtttcggcaa tgacgccgag gtttggatcg ccttctttca
    19681 cgcggaaaat cgacgcgccg cccataccgt ccagcggctt aagaatgatg tcgctgtgtt
    19741 tctcccagaa cgcttttagc tgcgctttat tgcgcgtaac cagcgtttct ggcgttaagt
    19801 cagagaacca ggcggtaaac agtttctcgt tacagtcgcg caggctctgc ggcttgttaa
    19861 cgatcagcgt ccctttctct tcggcacgtt ccagaatata ggtcgcgtag ataaactcgg
    19921 tatcaaacgg cgggtcttta cgcatcagga tcacatcgag atcggccagc ggcagatcct
    19981 gttcaccgac gaatgaaaac cactcttcgt agttctgttt cacgttcagc gtgcgggtat
    20041 gggcgcgggc ttcgccattg atcagataca gatcgcccat ctccatatag tgaagttcgt
    20101 aaccacgacg ctgtgcttcc agcaacatag cgaaactgga atctttcttg atgttgatgt
    20161 ttgcgatggg gtccatcacg atgccgagct tgatcattat tcttctccgt tagcccaaat
    20221 cgccaaatcg tacttgtagc gcggtaatgg cggtgagcgc agttgtctct gtacgcaaaa
    20281 cgcgaggtcc caacaggata tcagtaaatt gatagcgggc agtcatggca atttcatctg
    20341 ccgataaacc gccttccggg ccaatcagca ggcggacgcg ttcaaccggt aaaggcaacg
    20401 tattgatgct gttactggcg cgcgggtgaa gattcagttt cagtccttca tcctgctctg
    20461 cacaccaggc ttccagatcc atcgctggac ggatttccgg cacccggtta cgaccacact
    20521 gctcacaggc agcaattgca atcttctgcc actgctggag cttcttgttc agacgttcac
    20581 tatccagttt aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt acaccgagtt
    20641 cgatcgattt ctggatcgta aattccattt tttcaccacg cgacatcacc tgaccgaggt
    20701 gaatatgcag cggagattcg cgatcgtcga tctggccttc cagcaccttc acttccacgc
    20761 tttttttgct ggcgctggta atttcggcgt caaagacctg gttgctgccg tcaaacaatt
    20821 gcaacgcctg tcccggcccc atacgcagta cgcgcccaat atggttggcg gcatcttcgc
    20881 aaagcgcgat gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg gggatacgca
    20941 tagttaaaaa tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg ggttaacaaa
    21001 aagaatcccg ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg cacatacggg
    21061 ttatgattgc cctgtacctt cgcgatgcgt tcatcgcgct cgcactccca gtcggtaacc
    21121 ggatacatct tgtcccatgc gttgaacagc tgcgtttgct ggcgagagag tgtcaggttg
    21181 taatggtcac gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg cgctggtggt
    21241 tcggcagctt tttctttgaa atcgaccttc atggcgcatt gaccgtactg gccttcaccg
    21301 ccattccact ggctgtacat aaagttgccg cgatcgccat tcacctcgcc gactgacggt
    21361 tgcaggttat gcatatcgct ttccatcttg cgatagaccg gatctttggc gcaattttta
    21421 cgtccaccgt cctgccagca ctggcgctgg tgaccgaatt gccaggcggg aacgacgtgt
    21481 tcccactcta cgcggctggc gcggttttca tttttgcgca cctgatagcc gcacgattgc
    21541 agatcgacaa cgcctttttt gccctgccag ttaattttac atccgcaata aaacgtaccg
    21601 ggcgcgtcag cgtggacttt taccgccgcg gctttcgcct gagaaaaact attgatacct
    21661 tcggccaacg ccgggccgga aaatgctgcg ctcagtacca ccgcagcaat agacaaataa
    21721 cggtacatca cttactccgt gttaaaacga gccagcaacg tagcgaaagt tgttttggta
    21781 tgcaatcagc cagatcagga aagttcctga tagttcagat ggttattttg cgaccagttg
    21841 ttcgccgcag tgaacacagc gatagacggc ttcgccacga actacgcgat tatgacggcg
    21901 tacggtaagc tgatgttcct gacacttaca gctgtagggg aaggtgttgc ggcgcacgga
    21961 ttgcagttcg aactgatgcg tacggcgggc tggaacgccc agcacacttt ccatcatcca
    22021 cttccactct ttgccatgtg gtgctacgcg gccaaaatgt ttccatacca gcaaatgtgc
    22081 cagttcgtgc ggtaccactt cttcaataaa ggcttcactg ttttccaaca gcaaaacggg
    22141 attaaggcga atttcatagc tttccagcca ggctgttccg gcagaggttc cgcgctgggt
    22201 gtaagagagt tttggctccg ggtagttacg ccccaatttc aggttggcct gggcgagttt
    22261 ttcccgcagg cgacgcataa cggcctgctg gatggcgata gggagacggg atgttttcat
    22321 agcggcagag gatagagcga agaaaagatg actgcaagag gtggcttcct ccacgacggg
    22381 aggaagcctg gggagattaa tcgtgagcgc cgatttcgcg cagtttacga cctttcatca
    22441 gattacgttc aatatgttcc agcgaaacgt gtttggtttc tggcaccagc cacagtgtca
    22501 gcaggataaa caggacgttc agacccgcgt aaacccagaa ggtattagcg ttgcccagcg
    22561 tgttgagcat ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag ttggtggcgg
    22621 tggagcaggt gatgccaaaa tcgcggcctt tcagcggctg aatttcggag cacagtaccc
    22681 aaatcagcgg accggcactc atggcaaaac cgacaataaa catcagcagc atggcgatgg
    22741 cgaaatactg cgccgacgga gagtggatac cgatatgcat cattgtaccg agtacgccca
    22801 taccagcagc catcaccagg aagcccagcg ttagcgttgg tttacgtccc cagcggtcaa
    22861 caaggccgat tgcgataaag gtggcaagta cgttggtcag gccgacaatc actgtccccc
    22921 acatttgctc ggtggtgttg gtataacccg ccagttcgaa gattttcggc gcgtaataca
    22981 tgatgacgtt catcccggtg aattgctgca ttacctgtaa cagtacgcca aggaacaccg
    23041 cgcggcggaa gttgctattc tctttaaaca gcgcccagcc actctgttta acctgcaaac
    23101 tttcacggat ttcatccagt tcgcgtttcg cttccgcgct ggtgtcacgc aggcgtagca
    23161 gcacgcgttc ggcatcaaca aaacggcgtt tggcggcaaa ccaacgtggg ctgtctggca
    23221 ggaagaagac accaatcagc agcaaaattg ccgggatgat aatcacaccc agcatccagc
    23281 gccatgcacc ggtgtagctg aaggcggtat cagaaagata agcaccgagg atcccgattg
    23341 tgatcattaa ctgatacatc gagatcatac tgccgcgaat tttttccggc gcaatttcag
    23401 agaggtacag tggtgcggta taagaggcca cacctaccgc cagccccagc agaacgcggg
    23461 aaagaatcag tacttcaacg tttggcgcag ccgcagagaa cagcgaaccg gcaacaaaca
    23521 aaattgcgcc gatcatcagg ctctttttgc gcccgagttt aaaggagagc cagccgctgc
    23581 ccaccgcacc gactgccgca ccgaacatca tggagcttac gacccattct tgcgtgtgcg
    23641 aagtaatctg gaattcatct gcaataaacg gcagtgcgcc agcaattaca ccgatatcca
    23701 ggccaaagag taatcccgcc agagcggcaa ggaagcagac gaaaaacgtc attgccttgt
    23761 ttgactgccc ctgtttttta gcgtcaggca tgatgccctc caatatggtt attttttatt
    23821 gtgaattaag ataggtgagt acgacgtaaa aagatgtgaa gcaaatcaca tcagtgtaat
    23881 cgcttacact aatatttatc ttagttatga tttaagtgca tgaaaataat tgagttatat
    23941 catcatagat taaacgctgt tatctgcaat taagacttta ctgaaaagaa atgtaacaac
    24001 tgtgaaaacc gaataataat atggaaatat tacaaatctt tgtctgttgt ggttggtgta
    24061 atcggtttca gttgtgatga taatttgcgg tgaaacgtga tcaggaagaa tgatgttatt
    24121 gtgcaggcgg tttttttgcc ggatgcggcg tgaacgccta tccaccgcgg tgaggtaaaa
    24181 gaaaaaggcc agcgcgaggc tggcctttga aagcaggtga agaaagatta cttcagaccg
    24241 gcagcatcgc gcagcagctg cgctttgtcg gttttttccc acgggaaatg ttcacgacca
    24301 aagtgaccgt atgctgcggt ttctttgtag atcgggtgca gcagatccag catctgaatc
    24361 agaccgtatg ggcgcaggtc gaagaactca cgtaccagca gagtcagttg ttcagaaggc
    24421 actttctcag taccgaaagt ttctaccatg atggaagtcg gttcagccac gccgattgcg
    24481 taggaaacct gaatttcaca acgatcggcc aggccagcag caacgatgtt tttcgcgaca
    24541 taacgtgctg cgtaggctgc ggaacggtcc acttttgatg gatctttacc agagaatgca
    24601 ccgccaccgt gacgcgccat gccgccgtag gtatcaacga taattttacg accagtcaga
    24661 ccgcagtcac ccatcgggcc accgataaca aaacgaccgg tcgggttgat gaagaatttg
    24721 gtggcagaag tcagccattc agcgggcaga atcggcttga tgatctcttc cattaccgct
    24781 tcttgcagcg atttctggtc gatctcttca gagtgctgag tggaaagcac gacagcatcg
    24841 ataccaacga ttttgccgtc gtcatactgg aaagtcacct ggcttttcgc gtccgggcgc
    24901 agccacggca gagtgccgtt tttacgcact tcagcctgac gctgtaccag acggtgtgca
    24961 taggtgatag gtgctggcat cagcacgtcg gtttcattag ttgcgtagcc aaacatcaga
    25021 ccctggtcac ccgcgccctg ttccagcgga tcggcacggt caacgccctg gttgatgtca
    25081 ggagactgtt tgccgatagc gctcagaact gcacaggagt tagcgtcaaa gcccatgtcg
    25141 gaatgcacat agccaatttc gcgaacggtg ttacgggtga tctcttcgat gtctacccag
    25201 gcgctggtgg tgatttcgcc gccaactaaa accatgccgg tttttacgta ggtttcgcaa
    25261 gcaacgcgtg ctttcggatc ctgttcgagg atcgcgtcta aaacggcatc agagatttgg
    25321 tcagcaattt tgtcaggatg cccttcagag acggactcgg acgtaaaaag gtgttttgcc
    25381 atatttaata tcacctaaag agaatttggt tagctcaaac tgttgtgtgg attttctgtg
    25441 gtagcggatc ctaccacgac tctgcaggtt aaaaacactg gcagtctgag tgttaatcgg
    25501 tatggatgga ttaacatctg gatggctatt ttaggtcaat tcttcacccg atttccactt
    25561 ttttttgaat cgtgtctcat tctgttaaaa acgtggctgg aaatttttcc tgacaatgcc
    25621 ggcattctgc gtatttatct tttgcaattt tctgccattg tggggtataa aacgcggcgc
    25681 gcggcttaaa taaaaagcac acgacgtttc tttcgtgttg ccacttccag ccgggttgaa
    25741 atcagagttt tggcttgtgg gttcgtctta acaggcggcc gtggaggtga tacgaaataa
    25801 tgaaccgttg tctgctgctt aacctgtctc accgttctgg tgaagattcg ttccccgcac
    25861 tctgcatctc tgctttgcat acctgccgat gttataccca tctcggcgct tctcaggatt
    25921 caagagctgg ttacagttac tgaggactga acaagggcgc tcttgtaaaa acaagagttt
    25981 tctcgtggtt tcgccgaact gtcacactta cgttcggtta tgtgcttaat aatgttatga
    26041 aaaagaaacc ggttgcgcag ttggagcgtc agcattcact gctggaaaat ccatgtgctt
    26101 atgggttgtt atcgcagttc caggctgcga tagtcgttaa ctgttttaca cttaataaaa
    26161 taatttgagg ttcgctatgt ctgacgacat gtctatgggt ttgccttcgt cagcgggcga
    26221 acacggtgta ctacgctcca tgcaggaggt tgcaatgagc tcccaggaag ccagcaagat
    26281 gctgcgtact tacaatattg cctggtgggg caataactac tatgacgtta acgagctggg
    26341 ccacatcagc gtgtgcccgg acccggacgt cccggaagct cgcgtcgatc tcgcgcagtt
    26401 agtgaaaact cgtgaagcac agggtcagcg tctgcctgca ctgttctgtt tcccacagat
    26461 cctgcagcac cgtttgcgtt ccattaacgc cgcgttcaaa cgtgcgcggg aatcctacgg
    26521 ctataacggc gattacttcc ttgtttatcc gatcaaagtt aaccagcacc gtcgcgtgat
    26581 tgagtccctg attcattcgg gcgaaccgct gggtctggaa gccggttcca aagccgagtt
    26641 gatggcagtg ctggcacatg ctggcatgac ccgtagcgtc atcgtctgca atggttataa
    26701 agaccgcgaa tatatccgcc tggcattaat tggcgagaag atggggcaca aggtctatct
    26761 ggtcattgag aagatgtcag aaatcgccat tgtgctggat gaagcagaac gtctgaatgt
    26821 cgttcctcgt ctgggcgtgc gtgcacgtct ggcttcgcag ggctccggta aatggcagtc
    26881 ctccggcggg gaaaaatcga agttcggcct ggcggcgact caggtactgc aactggttga
    26941 aaccctacgt gaagccgggc gtctcgacag cctgcaacta ctgcacttcc acctcggttc
    27001 gcagatggcg aatattcgcg atatcgcgac aggcgttcgt gaatccgcgc gtttctatgt
    27061 tgagctgcac aagctgggcg tcaatattca gtgcttcgac gtcggcggcg gtctgggcgt
    27121 ggattatgaa ggtactcgtt cgcagtccga ctgttcggtg aactacggcc tcaatgaata
    27181 tgccaacaac atcatctggg cgattggtga tgcgtgtgaa gaaaacggtc tgccgcatcc
    27241 gacggtaatc accgaatcgg gtcgtgcagt gactgcgcat cacaccgtgc tggtgtctaa
    27301 tatcatcggc gtggaacgta acgaatacac ggtgccgacc gcgcctgcag aagatgcgcc
    27361 gcgcgcgctg caaagcatgt gggaaacctg gcaggagatg cacgaaccgg gaactcgccg
    27421 ttctctgcgt gaatggttac acgacagtca gatggatctg cacgacattc atatcggcta
    27481 ctcttccggc acctttagcc tgcaagaacg tgcatgggct gagcagcttt atttgagcat
    27541 gtgccatgaa gtgcaaaagc agctggatcc gcaaaaccgt gctcatcgtc cgattatcga
    27601 cgagctgcag gaacgtatgg cggacaaaat gtacgtcaac ttctcgctgt tccagtcgat
    27661 gccggatgcg tgggggatcg accagttgtt cccggttctg ccgctggaag ggctggatca
    27721 agtgccggaa cgccgcgctg tgctgctgga tattacctgt gactctgacg gtgctatcga
    27781 ccactatatc gatggtgatg gtattgccac gacaatgcct atgccggagt acgatccaga
    27841 gaatccgccg atgctcggtt tctttatggt cggcgcatat caggagatcc tcggcaacat
    27901 gcacaacctg ttcggtgata ccgaagcggt tgacgtgttc gtcttccctg acggtagtgt
    27961 agaagtggaa ctgtctgacg aaggcgatac cgtggcggac atgctgcaat atgtacagct
    28021 cgatccgaaa acgctgttaa ctcagttccg tgatcaagtg aagaaaaccg atcttgatgc
    28081 tgaactgcaa caacagttcc ttgaagagtt cgaggcaggt ttgtacggtt atacctatct
    28141 tgaagatgaa taattaagat tattctgctg gcgggtcgat ttatttacgt aaatcggt
//
LOCUS       DABSYT020000057        28083 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.057, whole genome shotgun sequence.
ACCESSION   DABSYT020000057 DABSYT020000000
VERSION     DABSYT020000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.057"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..209)
                     /locus_tag="ID725_RS14665"
                     /old_locus_tag="pgaptmp_002933"
     CDS             complement(<1..209)
                     /locus_tag="ID725_RS14665"
                     /old_locus_tag="pgaptmp_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGV7851477.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ID725_RS14670"
                     /old_locus_tag="pgaptmp_002934"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ID725_RS14670"
                     /old_locus_tag="pgaptmp_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGV7851478.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ID725_RS14675"
                     /old_locus_tag="pgaptmp_002935"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ID725_RS14675"
                     /old_locus_tag="pgaptmp_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGV7851479.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ID725_RS14680"
                     /old_locus_tag="pgaptmp_002936"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ID725_RS14680"
                     /old_locus_tag="pgaptmp_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGV7851480.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ID725_RS14685"
                     /old_locus_tag="pgaptmp_002937"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ID725_RS14685"
                     /old_locus_tag="pgaptmp_002937"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGV7851481.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ID725_RS14690"
                     /old_locus_tag="pgaptmp_002938"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ID725_RS14690"
                     /old_locus_tag="pgaptmp_002938"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGV7851482.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ID725_RS14695"
                     /old_locus_tag="pgaptmp_002939"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ID725_RS14695"
                     /old_locus_tag="pgaptmp_002939"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGV7851483.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ID725_RS14700"
                     /old_locus_tag="pgaptmp_002940"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ID725_RS14700"
                     /old_locus_tag="pgaptmp_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGV7851484.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ID725_RS14705"
                     /old_locus_tag="pgaptmp_002941"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ID725_RS14705"
                     /old_locus_tag="pgaptmp_002941"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGV7851485.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ID725_RS14710"
                     /old_locus_tag="pgaptmp_002942"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ID725_RS14710"
                     /old_locus_tag="pgaptmp_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGV7851486.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ID725_RS14715"
                     /old_locus_tag="pgaptmp_002943"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ID725_RS14715"
                     /old_locus_tag="pgaptmp_002943"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGV7851487.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ID725_RS14720"
                     /old_locus_tag="pgaptmp_002944"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ID725_RS14720"
                     /old_locus_tag="pgaptmp_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGV7851488.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ID725_RS14725"
                     /old_locus_tag="pgaptmp_002945"
     CDS             complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ID725_RS14725"
                     /old_locus_tag="pgaptmp_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGV7851489.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ID725_RS14730"
                     /old_locus_tag="pgaptmp_002946"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ID725_RS14730"
                     /old_locus_tag="pgaptmp_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGV7851490.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ID725_RS14735"
                     /old_locus_tag="pgaptmp_002947"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ID725_RS14735"
                     /old_locus_tag="pgaptmp_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGV7851491.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ID725_RS14740"
                     /old_locus_tag="pgaptmp_002948"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ID725_RS14740"
                     /old_locus_tag="pgaptmp_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGV7851492.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ID725_RS14745"
                     /old_locus_tag="pgaptmp_002949"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ID725_RS14745"
                     /old_locus_tag="pgaptmp_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGV7851493.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ID725_RS14750"
                     /old_locus_tag="pgaptmp_002950"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ID725_RS14750"
                     /old_locus_tag="pgaptmp_002950"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGV7851494.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ID725_RS14755"
                     /old_locus_tag="pgaptmp_002951"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ID725_RS14755"
                     /old_locus_tag="pgaptmp_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGV7851495.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ID725_RS14760"
                     /old_locus_tag="pgaptmp_002952"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ID725_RS14760"
                     /old_locus_tag="pgaptmp_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGV7851496.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ID725_RS14765"
                     /old_locus_tag="pgaptmp_002953"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ID725_RS14765"
                     /old_locus_tag="pgaptmp_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGV7851497.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ID725_RS14770"
                     /old_locus_tag="pgaptmp_002954"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ID725_RS14770"
                     /old_locus_tag="pgaptmp_002954"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGV7851498.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ID725_RS14775"
                     /old_locus_tag="pgaptmp_002955"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ID725_RS14775"
                     /old_locus_tag="pgaptmp_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGV7851499.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ID725_RS14780"
                     /old_locus_tag="pgaptmp_002956"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ID725_RS14780"
                     /old_locus_tag="pgaptmp_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGV7851500.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ID725_RS14785"
                     /old_locus_tag="pgaptmp_002957"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ID725_RS14785"
                     /old_locus_tag="pgaptmp_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGV7851501.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ID725_RS14790"
                     /old_locus_tag="pgaptmp_002958"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ID725_RS14790"
                     /old_locus_tag="pgaptmp_002958"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGV7851502.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ID725_RS14795"
                     /old_locus_tag="pgaptmp_002959"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ID725_RS14795"
                     /old_locus_tag="pgaptmp_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGV7851503.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ID725_RS14800"
                     /old_locus_tag="pgaptmp_002960"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ID725_RS14800"
                     /old_locus_tag="pgaptmp_002960"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGV7851504.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ID725_RS14805"
                     /old_locus_tag="pgaptmp_002961"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ID725_RS14805"
                     /old_locus_tag="pgaptmp_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGV7851505.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ID725_RS14810"
                     /old_locus_tag="pgaptmp_002962"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ID725_RS14810"
                     /old_locus_tag="pgaptmp_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGV7851506.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ID725_RS14815"
                     /old_locus_tag="pgaptmp_002963"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ID725_RS14815"
                     /old_locus_tag="pgaptmp_002963"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGV7851507.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6778 a         7552 c         7112 g         6641 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcctgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggccggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagatcca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccattt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctact ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccgc aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct agcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat aagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc ataccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcatc accgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacagta atgcgcattt
     8221 tatttgccat caacaaacga ccaatcacct gcccaaaacg tccaaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagccttta aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgcct gcaataaacg
     8761 taccgagcgc catcgacagc cccaacgcat ccataaacaa tgcggaaccc aataccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcaagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcaggacat cgctcatcgg
     9661 gtaacgattc aacgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacact
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 tggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gctgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgtcgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaat ctggtccccg gttcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagtggtg agataggcct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcgcct attcaacatc tggcacgttt agcgccgcag gaactggagc aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgaccttgat atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcgact ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggc catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccggttgat
    12601 gcggcggtta ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagcc gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt acctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg attcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtgcccgg
    14161 cggcaaaatg ggtctggcaa ttgaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggctgtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctcaatc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atattaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccttttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccga cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctattggc acttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct
    16741 gaaaagttat ttgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcttga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cgcaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga ccttgatgct gattaccacc cttgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggatccg caaccaacgc cactggcctg gcagcgaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaacca gcgttcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag tcagtttatt gttgaacata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat gcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cctgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgatggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacgc aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttatg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacttcaaag cacgctggta acgagtcagg ttccactacc
    19321 agcgaatgat agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ctttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca acgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgact cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctgaaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgaaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagcgaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaat gatgctggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccggcac atatggcgtg gtcaattctc gccctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctctaaccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aattaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgagagcatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagcaaag agatctacaa acgccttatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgtc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactatt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggttacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc
    25141 gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaataacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cctgattttt acgctcggta atttgctcga
    26041 tccatccgta cgccctctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgagctgt tcaccggaca caccatgttc ctcacctttg gggtgaaagc
    26161 gggcaccatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggtggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgtctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgcaat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc tgccagcttc attatgccgt cgattattga
    27061 ccgctcaccg ctgatggtgg cggtttcctc tggcggtact tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gctgcatctg ggccaggtag cgaaatacgc
    27181 cgggcagtta cgcggtcggg taaaacaaca gttcgccacg atgggtgaac gccgccgctt
    27241 ctgggaaaaa ctgttcgtta acgaccgtct ggcgcagtcg ctggcgaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gtctgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggatc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 gcaggaggag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggc ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctcagc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tagcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacac aactgggcga actggcgcag caaatgaata gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 att
//
LOCUS       DABSYT020000058        27823 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.058, whole genome shotgun sequence.
ACCESSION   DABSYT020000058 DABSYT020000000
VERSION     DABSYT020000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.058"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            99..761
                     /gene="acrS"
                     /locus_tag="ID725_RS14820"
                     /old_locus_tag="pgaptmp_002964"
     CDS             99..761
                     /gene="acrS"
                     /locus_tag="ID725_RS14820"
                     /old_locus_tag="pgaptmp_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HGV7851508.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(764..943)
                     /locus_tag="ID725_RS14825"
                     /old_locus_tag="pgaptmp_002965"
     CDS             complement(764..943)
                     /locus_tag="ID725_RS14825"
                     /old_locus_tag="pgaptmp_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGV7851509.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1027..1911)
                     /gene="yhdJ"
                     /locus_tag="ID725_RS14830"
                     /old_locus_tag="pgaptmp_002966"
     CDS             complement(1027..1911)
                     /gene="yhdJ"
                     /locus_tag="ID725_RS14830"
                     /old_locus_tag="pgaptmp_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGV7851510.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            complement(1996..2292)
                     /gene="fis"
                     /locus_tag="ID725_RS14835"
                     /old_locus_tag="pgaptmp_002967"
     CDS             complement(1996..2292)
                     /gene="fis"
                     /locus_tag="ID725_RS14835"
                     /old_locus_tag="pgaptmp_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGV7851511.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2318..3283)
                     /gene="dusB"
                     /locus_tag="ID725_RS14840"
                     /old_locus_tag="pgaptmp_002968"
     CDS             complement(2318..3283)
                     /gene="dusB"
                     /locus_tag="ID725_RS14840"
                     /old_locus_tag="pgaptmp_002968"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGV7851512.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3612..4493)
                     /gene="prmA"
                     /locus_tag="ID725_RS14845"
                     /old_locus_tag="pgaptmp_002969"
     CDS             complement(3612..4493)
                     /gene="prmA"
                     /locus_tag="ID725_RS14845"
                     /old_locus_tag="pgaptmp_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGV7851513.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4505..5956)
                     /gene="panF"
                     /locus_tag="ID725_RS14850"
                     /old_locus_tag="pgaptmp_002970"
     CDS             complement(4505..5956)
                     /gene="panF"
                     /locus_tag="ID725_RS14850"
                     /old_locus_tag="pgaptmp_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGV7851514.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5946..6188)
                     /locus_tag="ID725_RS14855"
                     /old_locus_tag="pgaptmp_002971"
     CDS             complement(5946..6188)
                     /locus_tag="ID725_RS14855"
                     /old_locus_tag="pgaptmp_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGV7851515.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6297..7646)
                     /gene="accC"
                     /locus_tag="ID725_RS14860"
                     /old_locus_tag="pgaptmp_002972"
     CDS             complement(6297..7646)
                     /gene="accC"
                     /locus_tag="ID725_RS14860"
                     /old_locus_tag="pgaptmp_002972"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGV7851516.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7657..8127)
                     /gene="accB"
                     /locus_tag="ID725_RS14865"
                     /old_locus_tag="pgaptmp_002973"
     CDS             complement(7657..8127)
                     /gene="accB"
                     /locus_tag="ID725_RS14865"
                     /old_locus_tag="pgaptmp_002973"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGV7851517.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9105..10079)
                     /locus_tag="ID725_RS14870"
                     /old_locus_tag="pgaptmp_002974"
     CDS             complement(9105..10079)
                     /locus_tag="ID725_RS14870"
                     /old_locus_tag="pgaptmp_002974"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGV7851518.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10231..12171
                     /gene="csrD"
                     /locus_tag="ID725_RS14875"
                     /old_locus_tag="pgaptmp_002975"
     CDS             10231..12171
                     /gene="csrD"
                     /locus_tag="ID725_RS14875"
                     /old_locus_tag="pgaptmp_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGV7851519.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12476..13519
                     /gene="mreB"
                     /locus_tag="ID725_RS14880"
                     /old_locus_tag="pgaptmp_002976"
     CDS             12476..13519
                     /gene="mreB"
                     /locus_tag="ID725_RS14880"
                     /old_locus_tag="pgaptmp_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGV7851520.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13585..14688
                     /gene="mreC"
                     /locus_tag="ID725_RS14885"
                     /old_locus_tag="pgaptmp_002977"
     CDS             13585..14688
                     /gene="mreC"
                     /locus_tag="ID725_RS14885"
                     /old_locus_tag="pgaptmp_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGV7851521.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            14688..15176
                     /gene="mreD"
                     /locus_tag="ID725_RS14890"
                     /old_locus_tag="pgaptmp_002978"
     CDS             14688..15176
                     /gene="mreD"
                     /locus_tag="ID725_RS14890"
                     /old_locus_tag="pgaptmp_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGV7851522.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15185..15778
                     /gene="yhdE"
                     /locus_tag="ID725_RS14895"
                     /old_locus_tag="pgaptmp_002979"
     CDS             15185..15778
                     /gene="yhdE"
                     /locus_tag="ID725_RS14895"
                     /old_locus_tag="pgaptmp_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGV7851523.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVVQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15768..17237
                     /gene="rng"
                     /locus_tag="ID725_RS14900"
                     /old_locus_tag="pgaptmp_002980"
     CDS             15768..17237
                     /gene="rng"
                     /locus_tag="ID725_RS14900"
                     /old_locus_tag="pgaptmp_002980"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGV7851524.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17305..21105
                     /gene="yhdP"
                     /locus_tag="ID725_RS14905"
                     /old_locus_tag="pgaptmp_002981"
     CDS             17305..21105
                     /gene="yhdP"
                     /locus_tag="ID725_RS14905"
                     /old_locus_tag="pgaptmp_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGV7851525.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21261..22706
                     /gene="tldD"
                     /locus_tag="ID725_RS14910"
                     /old_locus_tag="pgaptmp_002982"
     CDS             21261..22706
                     /gene="tldD"
                     /locus_tag="ID725_RS14910"
                     /old_locus_tag="pgaptmp_002982"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGV7851526.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22840..23769)
                     /gene="aaeR"
                     /locus_tag="ID725_RS14915"
                     /old_locus_tag="pgaptmp_002983"
     CDS             complement(22840..23769)
                     /gene="aaeR"
                     /locus_tag="ID725_RS14915"
                     /old_locus_tag="pgaptmp_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGV7851527.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23952..24155
                     /gene="aaeX"
                     /locus_tag="ID725_RS14920"
                     /old_locus_tag="pgaptmp_002984"
     CDS             23952..24155
                     /gene="aaeX"
                     /locus_tag="ID725_RS14920"
                     /old_locus_tag="pgaptmp_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGV7851528.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24163..25092
                     /gene="aaeA"
                     /locus_tag="ID725_RS14925"
                     /old_locus_tag="pgaptmp_002985"
     CDS             24163..25092
                     /gene="aaeA"
                     /locus_tag="ID725_RS14925"
                     /old_locus_tag="pgaptmp_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGV7851529.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25098..27065
                     /gene="aaeB"
                     /locus_tag="ID725_RS14930"
                     /old_locus_tag="pgaptmp_002986"
     CDS             25098..27065
                     /gene="aaeB"
                     /locus_tag="ID725_RS14930"
                     /old_locus_tag="pgaptmp_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGV7851530.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27157..27429
                     /locus_tag="ID725_RS14935"
                     /old_locus_tag="pgaptmp_002987"
     CDS             27157..27429
                     /locus_tag="ID725_RS14935"
                     /old_locus_tag="pgaptmp_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGV7851531.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27485..27748)
                     /gene="yhcN"
                     /locus_tag="ID725_RS14940"
                     /old_locus_tag="pgaptmp_002988"
     CDS             complement(27485..27748)
                     /gene="yhcN"
                     /locus_tag="ID725_RS14940"
                     /old_locus_tag="pgaptmp_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGV7851532.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6675 a         6958 c         7413 g         6777 t
ORIGIN      
        1 aatgttagcc tctcgtataa atacacatta ggtgatagat taaccttcgc tattttctca
       61 ctctgtgtcg aagatattta tttcctgaat aattaatcat ggcaaaaaga accaaagccg
      121 aagctctgaa gacccggcaa gaactgattg aaactgccat cgcccagttt gcgcaacatg
      181 gcgtaagcaa gacgacgctc aacgacattg ccgatgccgc taacgttacg cgtggcgcta
      241 tctactggca cttcgagaac aagacccaac tgtttaatga gatgtggttg caacagcctt
      301 cattgcggga gttaatccag gaacacttga cggctggatt agagcatgac ccgtttcaac
      361 aattgcgtga aaaattgatt gtcggcttgc aatatattgc caaaattccc cgccagcagg
      421 cgttgctgaa aatcttatat cacaaatgtg aatttaatga tgagatgctg gccgagggag
      481 tgatacgcga aaagatgggc tttaatccgc agactctccg cgaagtattg caggcgtgtc
      541 agcaacaagg ttgtgtagca aataacctcg atttagatgt tgtgatgatt attattgatg
      601 gtgccttcag cggaattgtt caaaactggt taatgaatat ggcgggttat gatctttata
      661 aacaagcccc cgctctggtc gataacgtat taagaatgtt catgccagat gaaaacataa
      721 cgaaattaat tcatcaaacg aatgaattaa gtgtcatgta aattcacatt ctgtcctgaa
      781 aattcagttc gtcacagtta accagtttta gtggattaac tgagttcata ttgcggcatt
      841 tgtctgtttc tgttgccagc agttcaatcc actgcatcag taaagtatca aacagaaaaa
      901 cgaagacagc gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat
      961 gtttaaccaa gtctggcagg aaatatacac gaaatgaata atctgcaaac caggcttaca
     1021 catcctttac tttgtaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag
     1081 gttgccggtc tttctttttt tcactttcgc cagttcttcc gcagagtaat gcgacgcgac
     1141 atccagccgt cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt
     1201 tcgtccgctg gcgatggcta cggcaccggt agtaaagcta ccagcaaacg ggtcgagaac
     1261 gatatcgcct gggttggaag aggcgagaat aatgcgtttc agtaaggctt ccggtttttg
     1321 cgtcgggtgg ttttcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt
     1381 acccggtact ttttgatgat tgtaaggctg tggaggattt ttgcgataat cgatcaacgc
     1441 gcgctgcgat ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt
     1501 tgcgtctttc accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg
     1561 cactccagaa ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca
     1621 ctggagatcg ataaagggca tgttttccgt actgttcatg atgtacatgc tgccctgctt
     1681 tttcagaacg cggtggcact ctgcaatcac ttctaacagc cagtcgataa acagatcttc
     1741 tttccaggct tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa
     1801 gatcagatcg acgctttcag taggtagctt tttaagttcg gcaagggcat caccgtgaat
     1861 aatggtctta gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc
     1921 ctttgtgaca cctataaaaa aggcgcttcc ccatgccgag tagcgccttt taatcaagca
     1981 attagctaac ctgaattagt tcatgccgta ttttttcaat tttttacgca gcgtaccacg
     2041 gttgatgccc atcatcagcg cagcacgggt ctggttacca cgggtgtatt gcatcaccat
     2101 gtccaacagg ggctgttcta cttcagccag taccagctca tagaggtcat tcacatcctg
     2161 accattcagt tgagcaaaat agttcttcag tgcctgttta accgagtcac gcaggggttt
     2221 ttgggttacc tgatcctgag agttaacggt agaaacggtc agtacgtcag aatttacgcg
     2281 ttgttcgaac atagttctgt cagctcttta tttctgttta cgcaaaattt tcgaagtatg
     2341 cctccaacgc ctccagctgt tcgctggcat cctcaatggc gttgaatgtg cgccgaaact
     2401 ggtcatttgg agcgtgttcc tggagatacc aggaaacgtg tttacgtgca attcggtacc
     2461 cttttgccgg accataaaag tcatgcagtt cccgaacgtg cgcgcaaagc aagcgcttaa
     2521 cctctgccaa aggcagcggg ggcagcaact ccccagtgtc cagataatgc tggatttccc
     2581 gaaagatcca gggtcttccc tgagctgcgc ggcctatcat cagggcatcc gcccctgtat
     2641 agtcgagcac agctctggct ttaagcgggt cagtaatgtc gccattcgcg ataaccggaa
     2701 tggaaacttt ctgcttaact gcccgaatac tgtcgtactc agcttctcca ttgaacaaac
     2761 aggcgcgtgt acggccatga atggtcagag cctgaatgcc acagtcttca gccagttggg
     2821 caatctcttc gcagttacgg tgttccggtg cccagccggt gcgaatcttc agggtaacag
     2881 gaacgtccac tgcattgacg acctcggtaa ggatcgattt aacgacatcc gggtactgca
     2941 agagggctga acctgcgagc ttgcgattca cttttttagc cgggcaaccc atattgatat
     3001 caataatctg ggcaccgctt tccacgttaa tacgtgctgc atctgccatt tctttcggat
     3061 cgctaccagc aatttgcacg gtgcgaatac cgggttcatc aatgtgcacc atccgtaaac
     3121 gagatttgtc gctttcccaa acctgtgggt tagaagacat catctcggat actgtcaatc
     3181 cggctcccat ctcgtagcac aacgtccgaa aaggtctgtc tgtaatgcca gccatgggcg
     3241 ctgcgatcag gcgatttctg agctgatatt gtccgatgcg catgagttaa gaaatgacca
     3301 tactgtgact gcaaggcggc gtatattacg cattttttgc acgagatgaa aggccaaact
     3361 ttgaccaatc ctctgagatg gatcaaagaa ttgcatttaa aatgagcgtg gtgcgataat
     3421 tactcataaa aatcatcata ttagaaaata gtgactaaaa tttacactca aagaaatttg
     3481 agtaagttct caatttttct ttatgaatga aaattttggc acgcaaattt gcgtaaataa
     3541 tcggcaaatt tacgtgcctt ttgtgagctt gctcgcactt cgccccgcgt caccctatgg
     3601 cgatgcgaag gttaattctt acgaccggta atacggcacc actcttcttt ttccacgacc
     3661 gggtccagag cgaagctgtc ggcataggct tcgcaaacgc tctccgcctg gcttgccaga
     3721 ataccagaaa ggcccagtaa gccgcctgaa actggcagga cgctaattaa cggtgccagt
     3781 tcacgtaatg ggcctgcaag gatgttagcg accaccacgt cggctttcat ttcttctggc
     3841 tgatctttcg gtaagtagag ttccagacgg tcagaaacgc cattacgttc ggcgttatcg
     3901 cggctggcct gaatcgcctg cggatcgata tcaataccaa tggcttttgc tgcacccagt
     3961 ttcagcgccg cgatcgccag aatgccggaa ccacagccaa agtcgatgac tgttttaccg
     4021 gttaaatcga ggctgtcgag ccattgcagg cacagagagg tggttggatg ggtacccgta
     4081 ccaaacgcca gccctggatc taacatcacg ttgacggcgt tttcgtccgg cacatcacgc
     4141 cagctagggc agatccacag tcgttcgcca aagcgcatcg ggtggaaatt atccatccat
     4201 tcgcgctccc agtctttatc ttctagttgt tcgattttat gcgcgaagcc tgcgccgagc
     4261 agcggatggt tttccagaat cgccaccacg tcgttcatat cggtttcagc gtcgaacaga
     4321 ccaatcacat cggtgtcgcc ccacaggcgc gtttcgcccg gcagcggctc aaatactggc
     4381 gtatcgtggg tatcctgaaa agtgatagaa acggcacccg cttccatcag cgcatcgcta
     4441 agatcttccg cgttcgcgcc ggtggtgttc agtttcagtt ggatccaagg catggcaaaa
     4501 ctctttattt atcagtagtc aaaacggtag cttgcgggac ggatgtaccg aaacggtttc
     4561 cgaccaggaa agccagcaaa cttagtagta acgagggcac gatagggtgg aagcccaggt
     4621 actgaatatt cagcgtcgcg agtacggcat acagcacgcc gccaacgatc atcgcactta
     4681 gcgcgccttt ggcgttggcg cgttcccagt aaagacccag caccagcggc cacaggaaaa
     4741 cggcttccag cccaccgaag gccagcaaat tcagccagat gatcatttct ggcggcttcc
     4801 aggcggcaag cagcagcaac gcgccgagaa ctaacgtaat taccgccgac atccgcttca
     4861 gacgcgtctc gttttgcatt tgatccggac ggatattcag atagagatct ttaatgatcg
     4921 tagcggaact ttgtagcaat tgggcgttaa ttgtcgacat gatcgcagcc ataggtgcag
     4981 ccagaaagat cccggcagca aacggtggca gcacttttac cattaacgtc gggatcacca
     5041 gatccggtac ggtgagatcg gggatcactg cacgacctaa cgctccggct aggtgcatac
     5101 cgaacatcag gattgcgact acaatcgtac cgatgatgat cccccggtgt actgctttgc
     5161 tgtctttata agagatacag cgcaccgcag tatgcggcag gcctataacg ccaaagcaca
     5221 ccagtaccca gaacgacgtc ataaaggcag gcgacagaat atcgtcagcg ccttgtggcg
     5281 taaccagttg cggatcgatg gtttgcaagg tctgtactgc gttgcttaag ccgccagcgg
     5341 catgtaccac gccaataagc agcacaacgg tgccaatcag catcacaagc ccttgcatgg
     5401 tgtcgttcag cacgctggcg cgaaagccac caaaggcggt atataacgca atgctgatac
     5461 caaaaatcag cagcccggtt tcataaggaa tacccgccgc ggtttccagc aggcgcgcac
     5521 cgccgataaa ctgcacggtc atcgcaccaa cgaacgcaac cagcaaactc aaactcgcca
     5581 gccacaccag aagacgactc tggtagcggg caaacagcat atcgttcagc gtcactgcat
     5641 tgtagcggcg cgcaagaatc gcaaacttct tgccgagaat accgagtgaa agccagactg
     5701 ccggaagctg aatcatcgcc agcaataccc agccaaggcc gtatttgtaa gcagctccag
     5761 gcccgccgat aaacgaactg gcactgatat aagtcgcggt gagcgtcatc gccagcacaa
     5821 taccgcccat agagcggctg ccgaggaaat actcattaag gaaggtgccg gtgctccgtt
     5881 tacgcatcgc ataaaccgag ataccgaaca ccaccaccag ataggcgacc agcggtagaa
     5941 ttacttcaag ctgcatcgtc atcctccagc gggatatcgc gatagataaa tttcaccatc
     6001 gcccagcaca gtccaataaa cagcagcggc gtcaggatgc aggccatctc aaaccagcgc
     6061 ggaaagccgg taaaaccggg ggcaacgcca ggtaagtaag cggctactaa ccaaactgcc
     6121 agatacaaaa gggtcagccc cagcgcccag cgcgcctctt tatgggcctg aacaaaacga
     6181 gtgtccattt tttgtccctg atgggtgaag aaagcgagga ttgtacctta tgggtgttgt
     6241 cgatccccag taataaaaaa ggccggaaaa tccggccttt tgacgctttt gcagtcttat
     6301 ttttcctgaa gaccgagttt tttctccaga tagtggatgt tagtgccacc atgctggaag
     6361 ttctcgtcat tcatgatgcg gatctgcaga tcaacgttgg ttttgatacc gtcgatgatc
     6421 agctcctgca gcgcattctt catgcgggca atcgccacgt cacggttttc accgtagcaa
     6481 atcagcttac cgatcattga gtcatagtac ggcggtacgg tgtagcccgc gtagatatga
     6541 gactcccaac gtacgccaaa accgccaggt gcgtggaaac gggtgatttt gcccggactt
     6601 ggcaggaagg tgttcggatc ttcggcgttg atacgacatt ccaccgcatg gccgcgaacg
     6661 tgaacttctt cttgcttgat cgacagcggt tgaccggcag cgatacgcag ctgttctttg
     6721 atcaggtcaa cgccggtgat catttctgta accgggtgtt ctacctgaat acgggtgttc
     6781 atttcgatga aatagaactc gccgttttcg aacaggaact cgaaagtacc tgcaccgcga
     6841 tagccgatat caacacacgc tttagcgcaa cgttcgccga tgtagcgacg cagttccggg
     6901 gtaatgcccg gtgctggtgc ttcttcgacc actttctggt ggcggcgctg catggagcag
     6961 tcacgttccg ccagatagat agagttgccc tgaccgtcag ccagtacctg aatctcgacg
     7021 tggcgaggat tttccaggta tttctccatg taaaccatat cgttgctgaa agcagctttc
     7081 gcttccgcac gggtcatgga gatggattgt gccagttcag cgtcgccgcg cactacgcgc
     7141 ataccgcgac caccgccgcc gccggaggct ttgataatca ccggataacc aatgcgttta
     7201 gcaatggcac ggtttttatc catatcgtcg cccagcgggc cgtcagaacc cggtacgcaa
     7261 gggacgcccg cttttttcat cgccgcgatt gcggatactt tgtcgcccat caggcgaatg
     7321 gtttctgctt tcgggccaat gaagataaag ccggagcgtt caacctgctc ggcaaagttg
     7381 gcgttctcgg agaggaagcc gtaacccgga tggattgcta ctgcgccggt gatttcagcg
     7441 gcgctgatga ttgccgggat gttcagataa ctttttactg acggagcagg gccaatacag
     7501 accgtttcat ctgccagtaa tacgtgtttt agatcgcgat ccgcgctgga gtgcacagcg
     7561 acagtcttga tgcccagttc tttacaggca cgaagaatac gcaatgcaat ctcgccgcgg
     7621 ttggcaataa caattttatc cagcatgttc gcctcgttac tcgatgacga ccagcggctc
     7681 gtcaaattct accggttgtc cactttcgac cagaattgct ttcacggtac cggatttgtc
     7741 cgcttcgatc tggttcatca ttttcatggc ttcaacgatg cacagggtat cgcccacgtt
     7801 gactttctga cccacttcga tgaacgcttt tgcatccggg cttggggtgc ggtagaaagt
     7861 accaaccatc ggggaacgta cgatgtgacc actgatttcc gctgctgctg gcgcttccat
     7921 ggaaggaacg gtcgccggag cggctgcgtt agattgagct ggctgctgca tcattggtgc
     7981 agcgtaagct tgttgcatca cagggaaact tgcggcagga gctgcacggc taatgcgtac
     8041 tgactcttcg ccttcagaaa tttccagttc ggagatgcct gattcttcaa ccagctcgat
     8101 cagtttttta atcttacgaa tatccatgag tgggttccgt actctttgtt tagtgtgatt
     8161 gtgacaggcg tttcaccgcc gtctgtaaag cgtatgcccc gacagctgta tgcatagcga
     8221 taaattccag caggtcggga cgccggtcta ttttgcccca gagtgtcaat gttagacttg
     8281 acggacattg tgcagcgatc tgctgctatc ctccggcaaa aaacaaaata taccttcgtt
     8341 gcgccaatgt caccttttcc gcagcgcaaa aactgcgtca gggaggacga ggtcgcacat
     8401 tataacgatt tcgtagcaat tggcagctaa atactggtct tatcagggaa gataatcaac
     8461 aactaacatg taaataacct tcaacaccgt gtaatttgca acaagccgca caattcacga
     8521 aattagcgcc atcatcgacg gaacttctta taacgtaagg tgaaaagctg taaagccagc
     8581 cctgcgaaga tgaggggctg caatgagata atcgtcacag accacaaata atgtagggat
     8641 gccaggatcg cgacaagata gacgacatta cgcagcggtt gctggggatt acccaatttt
     8701 cactgcatcg cctggataga tgtgaatgct aaagcacgca aaattaaccg attgacaatg
     8761 ctcagcatta aataggttcg agtgattaat ttgtagtcaa acaaaatcag attattcacc
     8821 tcatggtacc agaaataagt aaggggttaa gcgtagcgtc gcccaggcaa agcaccataa
     8881 acctaacagg cgacggaggc gtatcaataa tagctgttta gtggagtatc aatgcgcatc
     8941 aatttatggt cagtcgaaat catgactttc cagtttggat gatgttgtac aacttcatga
     9001 ccaaggtgtt cgacgaaagc tgtccggcta accgcttttc ccgttaaccg attgaataat
     9061 gatttcaaaa tgagttccct ctcttattat tccttgctaa atggttagtt aaccttcacc
     9121 agcgtgcgac cctggatctg gttattaatg atggcctcgg caaagttcgg tgcctctgac
     9181 agagatatct cttttgccgc ctgggtatag aatgattccg gtaaatcggc gaccagtcgc
     9241 tgccaggctt gtgcgcggcg ttctggtggc gtcattactg aatccacccc ttgcaaacgg
     9301 acattacgca gaataaatgg catgaccgtg gttggcagag taaaaccacc cgccagacca
     9361 caggccgcca cgcagccgcc gtaattcatt tgcgccagca cttttgccag cactttgtcg
     9421 ccaacagtgt caattgcccc agcccagact tgtttttcca gaggacggga ttcggcaaac
     9481 tcatcacgag ggagaatacg gctggcacct aaacttttca gatattcatg ggtactttcg
     9541 cgaccggaaa cggcaacgac ctgataaccc aacttatgca gcagcgccac ggcggtactg
     9601 ccgacgccac cactggcacc cgtcacgaca atctccccgt cctgcgggcg aacaccggca
     9661 tcttccagcg ccatcacaca cagcatggcg gtaaaaccgg cagtaccgat aatcattgct
     9721 ttacgcgcgt ccagcccttg cggcatggca accagccagt cacctttcac tcgcgcctgc
     9781 tccgccagcc caccccagtg gttttcacca acgccccagc cagtgagtaa cacctcctga
     9841 ccggcatgaa aacgcggatc ttcgctggtg cgtacagttc cggcaaaatc gatcccagga
     9901 atcatcggaa aattacggat gatttttccc ttaccggtaa tcgccagcgc atctttatag
     9961 ttcaggctcg accagtgaac atcgaccgtg acatcgccct ccggcaggcg actttcgtcc
    10021 agagtctgta ctgatgcgag agttttgccg tcctgctgtt ctaaaagtaa cgcctgcata
    10081 agtggtcctc atgtgcatga tggattggaa aataatttct gaagactata ctcgctaatg
    10141 gaaaagcaaa gccgatgaag cgcaataaat tgcgagataa atctgatttg ctagtatgcc
    10201 cgcttcctca ctatcggagt taacacaagg atgagattaa cgacgaaatt ttcggccttt
    10261 gttacgctgc tcaccgggtt aacaattttt gtgactttgc tgggctgttc gctaagtttc
    10321 tacaacgcca ttcagtataa gtttagtcat cgcgttcagg cggtggcgac ggcgattgat
    10381 acccaccttg tgtcgaatga cttcagcgca ttaaggccac aaattaccga attaatgatg
    10441 tcggcagata tcgttcgtgt agacctgctc catggtgata agcaggttta taccctggcc
    10501 agaaatggta gttatcgtcc ggttggcacc agcgatctgt ttcgtgaact gagcgttccg
    10561 ttgataaagc atccggggat gtcgttgcgt ctggtttatc aggatccgat gggcaactat
    10621 ttccattcgt tgatgaccac cgcgccgctc acaggggcga ttggctttat cattcttatg
    10681 ctctttctgg cggtacgctg gttacaacgg caacttgccg ggcaagaatt gctggaaacc
    10741 cgggctactc gtatcttaaa cggtgagcgt ggctctaatg tgttgggaac catctatgaa
    10801 tggccgccca gaaccagcag tgcgctggat acgctgcttc gtgaaattca gaacgcacgc
    10861 gaacaacaca gccgtcttga tacgctgatc cgctcttatg ccgcacagga cgtgaaaacc
    10921 ggcctcaata accgactctt tttcgataat cagttagcaa cgttactgga agatcaggag
    10981 aaagtaggta cccacgggat cgtgatgatg attcgtctgc cggatttcaa tatgttgagc
    11041 gatacctggg ggcacagcca ggttgaagaa cagttcttca ctctgacgaa tctgctgtcg
    11101 acatttatga tgcgctaccc tggcgcactg ctggcgcgtt accaccgcag tgattttgct
    11161 gcgctgttac cgcaccggac gttaaaagag gcagagagca tcgccggtca gttaatcaaa
    11221 gccgtcgata ccttgccgaa caataaaatg ctcgatcgcg acgatatgat ccacattggt
    11281 atctgcgcct ggcgtagtgg tcaggatacc gagcaggtaa tggaacatgc agagtctgcc
    11341 acgcgtaatg cgggattgca gggcggcaat agctgggcta tttacgatga ctcgttgcct
    11401 gaaaaaggac gcggtaatgt tcgctggcgt acgcttatcg agcaaatgct cagtcgcggc
    11461 ggcccgcgcc tttatcaaaa accggcggtt actcgcgaag gtcaggttca tcatcgcgaa
    11521 ctcatgtgcc gcatctttga tggtaatgaa gaggttagct cggcggagta tatgccgatg
    11581 gtcttgcagt ttggcttatc ggaagagtat gaccgtctgc aaatcagccg tcttattcca
    11641 ctattgcgtt actggccaga ggaaaatctg gcgattcagg ttaccgttga gtcgctgatt
    11701 cgcccgcgtt ttcagcgttg gctgcgcgat acgttaatgc aatgtgaaaa atcacaacga
    11761 aaacgcataa ttattgaact tgcagaggcc gatgtaggtc aacatatcag tcgtttacaa
    11821 cctgttattc gtttagtgaa tgctttaggg gtacgggtag ccgtcaacca ggctggtttg
    11881 acgctggtaa gcaccagttg gatcaaagaa cttaatgttg agttactcaa gctccatccg
    11941 gggctggtca gaaacattga gaagcgaacg gagaaccagc tgctggttca aagcctggtg
    12001 gaagcctgct cagggaccag cacccaggtt tacgccaccg gtgtgcgttc gcgaagcgag
    12061 tggcagaccc tgattcagcg cggtgttaca ggcgggcaag gggatttttt cgcgtcctca
    12121 cagccacttg atactaacgt gaaaaaatat tcacaaagat actcggttta acctgccgtt
    12181 taatccgttt tcacgtagaa taacgcgcgc tgcgtctcat gggagtgtgc ttgtctgctc
    12241 gccagattgt tgcagcacat atgcagatga atgaccttac gcggttgcaa acaggcgagg
    12301 aatgctgctg atgcattaag cctttctgga ctcaggcaga gatttgtaac aaaggaaacg
    12361 aactgcacta attttcaccg tagcagatga tttttgcgcc ttgtcgctgc tgcgtgtggt
    12421 tggtaaagta agcggatttt cttttccgcc ccagctttca ggattatccc ttagtatgtt
    12481 gaaaaaattt cgtggcatgt tttccaatga cttgtccatt gacctgggta ctgcgaatac
    12541 cctcatttat gtaaaaggac aaggcatcgt attgaatgag ccttccgtgg tggccattcg
    12601 tcaggatcgt gccggttcac cgaaaagcgt agctgcagta ggtcatgacg cgaagcagat
    12661 gctgggccgt acgccgggca atattgctgc cattcgccca atgaaagacg gcgttatcgc
    12721 cgacttcttc gtgactgaaa aaatgctcca gcacttcatc aaacaagtgc acagcaacag
    12781 ctttatgcgt ccaagcccgc gcgttctggt ttgtgtaccg gttggcgcga cccaggttga
    12841 acgccgcgca attcgtgaat ccgcgcaggg cgctggtgcc cgtgaagtct tcctgattga
    12901 agaaccgatg gctgccgcaa ttggtgctgg cctgccggtt tctgaagcga ccggttctat
    12961 ggtggttgat atcggtggtg gtaccactga agttgctgtt atctccttga acggcgtggt
    13021 ttactcctct tccgtacgca ttggtggtga ccgtttcgac gaagctatca tcaactatgt
    13081 gcgtcgtaat tacggttctc tgatcggtga agccaccgca gaacgtatca agcacgaaat
    13141 cggttcggct tatccgggcg atgaagtccg tgaaatcgaa gttcgtggcc gtaacctggc
    13201 agaaggtgtt ccacgcggtt ttaccctgaa ctccaacgaa atcctcgaag cactgcagga
    13261 accgctgacc ggtattgtga gcgcggtaat ggttgcactg gaacagtgcc cgccggaact
    13321 ggcttccgac atctccgagc gcggcatggt actcaccggt ggtggcgcac tgctgcgtaa
    13381 ccttgaccgt ttgttaatgg aagaaaccgg cattccagtc gttgttgctg aagacccgct
    13441 gacctgtgtg gcgcgcggtg gcggcaaagc gctggaaatg atcgacatgc acggcggcga
    13501 cctgttcagc gaagagtaat cggatgcagg caggggaagc gtctgtttac cctgcctggt
    13561 ctgatacgag aatacgcata acttatgaag ccaattttta gccgtggccc gtcgctacag
    13621 attcgcctta ttctggcggt gctggtggcg ctcggcatta ttattgccga cagccgcctg
    13681 gggacgttca gtcaaatccg tacttatatg gataccgccg tcagtccttt ctactttgtt
    13741 tccaatgctc ctcgtgaatt gctggatggc gtatcgcaga cgctggcctc gcgtgaccaa
    13801 ctagaacttg aaaatcgggc gttacgtcag gaactgttgc tgaaaaacag tgaactgctg
    13861 atgcttggac aatacaaaca ggagaacgcg cgtctgcgcg agctgctggg ttccccgctg
    13921 cgtcaggatg agcagaaaat ggtgactcag gttatctcca cggttaacga tccttatagc
    13981 gatcaagttg ttatcgataa aggtagcgtt aatggcgttt atgaaggcca gccggtcatc
    14041 agcgacaaag gtgttgttgg tcaggtggtg gccgtcgcta aactgaccag tcgcgtgctg
    14101 ctgatttgtg atgcgaccca cgcgctgcca atccaggtgc tgcgcaacga tatccgcgta
    14161 attgcagccg gtaacggttg tacggatgat ttgcagcttg agcatctgcc agcgaatacg
    14221 gatattcgtg ttggtgatgt gctggtgacc tccggtctgg gcggtcgttt cccggaaggc
    14281 tatccggtcg cggttgtctc ttccgtaaaa ctcgataccc agcgcgctta tactgtgatt
    14341 caggcgcgtc cgactgcagg gctgcaacgt ttgcgttatc tgctgctgct gtggggggca
    14401 gatcgtaacg gcgctaaccc gatgacgccg gaagaggtgc atcgtgttgc taatgaacgt
    14461 ctgatgcaga tgatgccgca ggtattgcct tcgccagacg cgatggggcc aaagttacct
    14521 gaaccggcaa cggggatcgc tcagccgact ccgcagcaac ccacgacagg aaatgcagct
    14581 actgcgcctg ctgcgcccac acagcccgct gctaatcgct ctctacaaag ggctacgccg
    14641 ccgcaaagtg gtgctcaacc gcctgcgcgt gcgccgggag ggcaatagtg gcgagctatc
    14701 gtagccaggg acgctgggta atctggctct ctttcctcat tgcgcttttg ctgcaaatca
    14761 tgccctggcc ggataacctg attgttttcc ggccaaactg ggtgttactc atcttgttgt
    14821 attggatcct ggccttgccc catcgcgtaa atgtgggcac aggttttgtg atgggtgcca
    14881 tactggatct gatcagcggc tcgacgcttg gcgtacgcgt attggcgatg agcatcattg
    14941 cttacctggt ggcgctgaaa taccagcttt tccgcaacct cgcattatgg cagcaggcgc
    15001 tggtcgtcat gttgctttcg ctggtggtgg atattattgt tttctgggca gagtttttag
    15061 tgattaacgt ctctttcaga ccggaagtgt tctggagtag tgtagtcaat ggggtgctct
    15121 ggccgtggat tttcttgctg atgcgtaaag tccgtcagca gtttgcagtg caataaaggt
    15181 ttctatgact tctctgtatt tagcttccgg ttctccgcgt cgtcaggagt tacttgcgca
    15241 acttggcgtg acctttgaac gtattgttac gggcattgag gagcagcgtc agccgcagga
    15301 gagcgcgcag cagtatgttg tgcgtctggc gcgcgagaaa gcacgggcag gtgtcgtgca
    15361 aacggcgaag gatctcccgg tgctgggtgc ggatactatc gttatcctga acggagaagt
    15421 gctggagaaa ccgcgcgacg cagagcatgc ggcgcagatg ttgcgcaaat tatcgggtca
    15481 gacccatcag gtgatgacgg cagtggcgtt ggccgacagc cggcacattc tcgattgcct
    15541 ggtggtcacc gatgtgactt tcagaacgtt aacagacgaa gacatcgcgg gctatgtcgc
    15601 cagcggtgaa ccgttagata aagcaggtgc atacggtatt caggggctgg gtggctgttt
    15661 tgtcaggaag ataaatggca gctatcaagc cgtagtcggc ttaccgctgg ttgaaacgta
    15721 tgaattatta agtaatttta acgcactgcg tgagaaaagg gataaacatg acggctgaat
    15781 tgttagtaaa cgtaacgcct tcggaaacgc gagtggcgta tattgatggc ggtattctgc
    15841 aggaaattca tattgaacgt gaggcgcgac gcggaatagt aggcaatatc tacaagggtc
    15901 gtgtaagtcg tgtacttccg ggtatgcagg cggcttttgt agatattggg ctggataaag
    15961 ccgcgtttct tcatgcatct gacatcatgc cgcacaccga atgtgtggcg ggtgaagaac
    16021 aaaagcaatt cacggtgcgc gacatctcgg aactggttcg tcaggggcaa gatctgatgg
    16081 tgcaggtggt gaaagatccg cttggcacta agggggcgcg cctgaccacc gatatcacgc
    16141 tgccttctcg ctatctggtc tttatgccag gggcttctca cgttggggtt tcccaacgaa
    16201 ttgaaagcga atcagaacgt gaacgcctga aaaaagtggt cgcagagtat tgcgacgagc
    16261 agggcgggtt tatcatccgt accgcagcgg aaggggttgg cgaggctgaa ctggcctccg
    16321 atgccgctta tctgaaacgc gtctggacca aagttatgga gcgtaaaaag cgcccgcaga
    16381 cccgttatca gctgtacggc gaactggcgt tggcgcagcg tgttctgcgt gatttcgccg
    16441 atgccgaact ggaccgcatt cgcgttgact cacgcctgac ttacgaagcg ttacttgagt
    16501 tcacctcgga gtacattccc gagatgacca gcaagctgga gcattacacc ggacgccagc
    16561 cgattttcga tctctttgat gtcgaaaacg aaatccagcg agcgctggaa cgcaaagttg
    16621 aactgaaatc cggtggttat ctgattatcg accagaccga agcgatgacc accgtggaca
    16681 tcaataccgg agcgtttgtc ggtcatcgca atctggacga caccattttc aataccaata
    16741 ttgaagcgac gcaggctatc gctcgccagt tacggttgcg taatctgggc gggattatca
    16801 ttattgattt catcgatatg aataatgaag atcaccgccg ccgagtgctg cactcgctgg
    16861 agcaggcgtt gagcaaagac cgggtgaaaa ccagcgttaa tggtttttcg gcgctggggc
    16921 tggtggagat gacgcgtaaa cgcacccgcg aaagcattga gcacgtactg tgtaacgaat
    16981 gcccaacctg ccacggtcgc ggaacggtga aaaccgtgga aacggtatgc tatgaaatca
    17041 tgcgcgagat tgttcgtgta caccatgctt acgactccga ccgtttcctg gtctatgctt
    17101 ctccggcagt agccgaagcc ttgaaaggcg aagagtcaca ctcgctggcg gaagtggaaa
    17161 ttttcgttgg caaacaggtt aaagtacaaa ttgaaccgct ctataaccag gagcagtttg
    17221 acgtcgtaat gatgtaaaca gatgctgggc cgccatccgg caaagggttt ttgagtcaca
    17281 tttttagcag acaaggagtg acgggtgagg cgattgccgg ggattttact gcttactgga
    17341 gccgcgctcg ttgtgatcgc tgccctgctg gttagcggcc tgcgtattgc tttaccgcat
    17401 cttgacgcct ggcgtccgga aatcctcaac aaaatagaat ccgcgactgg catgccggta
    17461 gaagccagtc agctctcagc cagctggcag aattttggcc cgacgcttga agcgcacgac
    17521 atccgtgcag aactaaagga tggcggcgaa ttttcggtta aacgcgttac tctggcgctg
    17581 gatgtctggc agagcctgtt acatatgcgc tggcagtttc gcgacctcac tttctggcag
    17641 ctgcgctttc gcaccaacac tcctatcacc agcggtggta gcgatgacag tctggaagcc
    17701 agtcacatca gcgatctgtt tcttcgtcaa tttgaccatt tcgatctccg cgacagtgaa
    17761 gtcagtttcc tgacgccatc cggtcagcgc gccgagctgg cgatcccaca actcacctgg
    17821 ctgaacgatc cacgtcgaca ccgtgcggaa ggcctggtaa gcctctccag ccttaccgga
    17881 cagcacggcg tgatgcaggt gcgcatggat ttgcgcgatg atgaggggtt gttaagcaat
    17941 ggtcgcgtct ggctccaggc ggatgacatc gacctgaagc cgtggctcgg taaatggatg
    18001 caggacaata ttgcgctgga aacggcgcag ttctcccttg aaggctggat gacgatcgac
    18061 aaaggcgatg taaccggcgg tgacgtctgg ctgaaacagg gtggtgccag ctggttgggc
    18121 gagaaggaaa cgcatacgct gtcggtggat aatctgaccg cgcatattac gcgtgaaaat
    18181 ccgggatggc agttctctat tcccgataca cggatcacga tggacggcaa accctggccg
    18241 agcggagcat tgacgctggc ctggataccg gaacaggacg ttggcggcaa agacaataaa
    18301 cgcagtgacg aactccggat tcgcgccagt aatctggagc tggcaggcct ggagggcata
    18361 cgcccgctgg ccgcgaaact ttcacctgca ctgggtgatg tttggcgctc tacacaaccg
    18421 agcggcaaga ttaacactct ggcgctggat atcccgcttc aggcggcaga caagacccgt
    18481 tttcaggcat cgtggagcga tctggcctgg aagcaatgga aattattacc gggtgcggaa
    18541 cacttctccg ggacgctttc cggcagcgtt gaaaatggtt tgcttaccgc gtcgatgaag
    18601 caggcaaaga tgccttacga aacggtattc cgtgcgccac tggaaatcgc cgacggccag
    18661 gcaactataa gctggctgaa caatgacaaa ggtttccagc tggatgggcg taatattgac
    18721 gttaaagcca aagccgtcca tgcgcgcggc ggttttcgtt acctgcaacc tgctaacgat
    18781 gaaccctggc tgggtattct ggctggcatc agtaccgatg atggttcaca agcctggcgc
    18841 tatttcccgg aaaacttgat gggtaaagac ctggttgatt acttaagtgg cgcgattcag
    18901 ggcggtgaag cggataacgc gacgctggtt tatggtggca atccgcaact cttcccctat
    18961 aaacacaacg aaggtcagtt tgaagtgctg gtgccgctgc gcaacgcgaa gtttgccttc
    19021 cagccggact ggcctgcatt aactaacctt ggtattgaac tggactttat taacgacggt
    19081 ttatggatga aaaccgatgg cgttaatctg ggcggcgtgc gcgcgagtaa tctcaccgca
    19141 gtgatccctg actactcaaa agaaaaactg ctgattgacg ctgacattaa aggtccgggt
    19201 aaagccgttg gcccttactt tgatgagaca ccgctgaaag attctctggg tgcgaccctg
    19261 caagaactcc agctcgacgg cgatgtgaat gctcgcttac atcttgatat cccgctgaac
    19321 ggcgagctgg taaccgcgaa aggtgaagtg acgctgcgta ataacagtct gtttatcaaa
    19381 ccactcgaca gcaccctgaa aaatttgagc ggtaaattca gctttatcaa tggcgatctg
    19441 caaagtgaac cactgacagc aagctggttt aatcagccgt tgaacgtgga tttttccacc
    19501 aaagaagggg caaaagccta ccaggtcgcg gtgaatctca acggtaactg gcaaccggcg
    19561 aaaaccggcg ttctgcctgc agcggtgaac gaagcattga gtggcagcgt ggcgtgggat
    19621 ggtaaagtgg gcattgttct gccttatcat gctggcgcga cgtataacgt agagctaaac
    19681 ggcgatttga agaatgtgag cagtcactta ccttcaccgt tagccaaacc tgcgggtgaa
    19741 ccactgccgg taaacgttaa ggttgatggc aatctcaaca gctttgaatt aaccggacag
    19801 gctggtgcgg ataatcattt caatagccgc tggttgctcg gtcaaaagct gacgctcgac
    19861 cgtgctattt gggcggcaga cagtaaaacg ctcccgccgt tgccggaaca aagtggtgtt
    19921 gaactcaata tgccgccgat gaatggtgcc gagtggctgg ccctgtttca gaaaggtgcg
    19981 gcggagagtg tcggtggtgc agcgagtttc ccacaacaca taacgttacg tacgcctatg
    20041 ttgtcgctgg gaaatcagca atggaataac ctgagtattg tttcgcaacc gacggcaaat
    20101 ggcacccagg ttgaggcgca ggggcgtgaa atcaacgcca cgctggcgat gcgtaataac
    20161 gcgccgtggc tggcgaatat caaatatctt tattacaacc cgagcgtggc gaaaactcgt
    20221 ggtgattcaa cgccgtcatc acctttcccg acaacggaac gcattaactt ccgtggctgg
    20281 tcggacgccc aaatacgatg cgcagagtgc tggttctggg ggcaaaaatt cgggcgcatt
    20341 gacagtgata tcaccatttc tggcaataca ttaacgctga ccaatggact gattgatact
    20401 ggtttttcgc ggctcactgc cgatggtgaa tggattaaca atccggggaa tgaacgtacc
    20461 tcgctgaaag gaaaactgcg cgggcagaaa attgatgccg ccgcagaatt ttttggtgtc
    20521 acgacgccca tacggcagtc gtcatttaat gtggattacg atttacactg gcgtaaagca
    20581 ccgtggcagc cagatgaggc gacgttgaat ggcatcattc atactcaact gggtaaaggc
    20641 gaaattaccg aaatcaatac cggacatgcc gggcaattgc tgcgcttatt gagcgtagat
    20701 gccctgatgc gtaagctgcg ttttgatttc agagacactt ttggcgaagg gttctatttt
    20761 gactccattc gcagcaccgc gtggattaaa gacggcgtta tgcacaccga cgacacgctg
    20821 gtggatggcc tggaggcgga tatcgccatg aaagggtcgg taaatctggt acgtcgcgac
    20881 ctgaatatgg aagcggttgt cgcaccagag atttctgcga cagtgggcgt ggctgcggct
    20941 tttgcggtta accccattgt tggcgcggca gtgtttgccg ccagtaaagt gctggggccg
    21001 ctgtggagca aagtctccat tttgcgctat cacatttcgg gtccgctgga cgatccgcaa
    21061 atcaacgaag tgttgcgcca accgcgtaaa gaaaaagcgc aatgatttga cgagggcgcg
    21121 taattgcccc aatctcatag gataatcgtt gccaaaggcc aacgagccag aacataaccg
    21181 taggctggat agggcgttca cgccgcatcc ggcagccgta aaaaatcctc tactgcagta
    21241 actaacgagt agcaaaaacg atgagtctta acctggtaag tgaacaattg ctagcggcga
    21301 acggcctgaa acatcaggac ttgttcgtga tcctcggtca actggccgaa cgtcgccttg
    21361 attatggcga tctctatttt cagtcgagct atcacgaatc ctgggtttta gaagaccgca
    21421 ttattaaaga tggttcttac aacatcgatc agggcgttgg tgtgcgtgca atcagcggtg
    21481 aaaaaaccgg atttgcttac gctgaccaaa tcagcctgct ggcgctggaa cagagtgcgc
    21541 aagcggcgcg caccatcgcc cgtgatagcg gtgatggcaa agtacagacg ctgggcgcgg
    21601 tagagcatag cccgttgtat acctcggtag atccgctgca aagcatgagc cgtgaagaga
    21661 agctggatat cctgcgtcgc gtcgataagg ttgcccgcga agcggacaag cgcgtacagg
    21721 aagtgactgc cagcctcagt ggtgtctatg aattaattct ggttgcggcc accgacggca
    21781 cgctagcggc ggatgtccgt ccgctggtgc gtctttccgt gagcgttctg atcgaagaag
    21841 atggcaaacg cgaacgcggt gccagtggcg gcggcggtcg ttttggttat gagttcttcc
    21901 ttgccgatct cgacggcgaa gtccgtgcgg atgcatgggc aaaagaagca gtacgtatgg
    21961 cgctggtcaa tctttctgcc gttgctgcac cagcgggcac catgccggta gtacttggcg
    22021 caggttggcc gggcgtgctg ttgcatgaag cggtaggtca cggtctggaa ggcgacttca
    22081 accgccgtgg cacttcagta tttagtggac aggtcgggga gctggtggct tcagaactgt
    22141 gtaccgtggt tgatgacggt acgatggtcg atcgccgtgg ttcggtggcg attgatgacg
    22201 aaggtacgcc aggccagtac aacgtgctga ttgagaacgg cattctgaaa ggctacatgc
    22261 aggataaact caacgcgcgt ttgatgggga tgacgccgac tggcaacggt cgccgtgaat
    22321 cctacgccca tctgcccatg ccgcgtatga ccaacaccta tatgctgccg ggtaaatcga
    22381 ccccgcagga aattattgaa tccgttgagt acggtatcta tgcaccgaac tttggtggcg
    22441 gtcaggtgga tatcacctcc ggcaaattcg ttttctccac ttcagaagca tatctgattg
    22501 aaaacggtaa agtaacgaag ccggtgaaag gcgcaacgtt gattggttcc ggtatcgaaa
    22561 ccatgcagca gatttcgatg gttggcaacg acctgaaact ggataacggc gtgggtgtct
    22621 gcggtaaaga agggcaaagt ttgccggttg gcgtgggcca gccaacgctg aaagttgata
    22681 acctgactgt tggcggtact gcgtaataat ttcagtattt tcagaaggat aattaatcct
    22741 tcctcgtgca cgaacggtcc cctcgcccct ttggggagag ggttagggtg aggggaaccc
    22801 gttggcacag gtttgtacaa cgtaacagta caatatgaat tacttctctt tcccgcgccc
    22861 gtgcatctcc tgaaacaatt taccgacctc aacaaaataa tccgtcagcg agttgatcac
    22921 gacctgtacc ttcagcggca gcttatcttt ttcggtatat aacgcataaa ccgggcgtgg
    22981 atctgactga taacgcggca gcaggatctc cagctcccca cgattgatct cgttgatcac
    23041 ccacatcagc ggcacgtagg cgatcccggc acccgccgtt agccagcgca ccagcgtcat
    23101 cggatcatta gtcacaaatc tcccttgcgg gatcaggcga gtcgagatcc cttccggtgc
    23161 aatcagttca aattcattgt cgggccgcac gctgtattca agccatgaat ggctacttaa
    23221 atcggcgggt ttttccggta tgccgtattg tgtgagatag cttttcgcgg cgcacaccac
    23281 cattggcatc gcgccaagac ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg
    23341 gatcaccaca tccagaccgt cggcaatcag gtcgggggct ggaattccgg taaccagatt
    23401 gacgctcaaa cctgggtatt ctttcagcat tttggctgtc agcccggcga gaacattttg
    23461 tgccatagtt gaagaacagc caatgcgtag cgtcccgatg ggggtgttat tgaaggcata
    23521 cagttgctca tgaacatcct gcacttcatg aagcatacga cggcagccct ggtagtaaat
    23581 tctaccggct tcggtcaggc caatgctgcg tgtgctacgg tttaacagct ttacctgcaa
    23641 ctcatcttcc agttttgaca ccgtctgact gatggacgaa acgctcatct gtagctgtct
    23701 ggcggcggcg gtaaaagagc caaattcaac tactttggca aacaccgaca tgcgttttag
    23761 tcgttccatt attcactctg acttaaaagt gatttagatc acataatata gataacagca
    23821 taacagttac gctaatatat taaatatcaa tctacagcaa tgttgctctc gcccggcttt
    23881 ccatgccctt ctctgcggcg acagatgctg aaaataataa cgcctgctct ctctatacaa
    23941 ccaaggtcaa catgagtctg tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga
    24001 tattttttga attgctttta tcactggcga ttttctggct ggtgtgccgg gtacttgtgc
    24061 caacaggtat ctacgacttt gtctggcatc cggcgttgtt caacaccgcg ctctattgct
    24121 gcttgtttta tttgatatcg cgactgttcg tttgaggttg aagtgaaaac actaataaga
    24181 aaattctccc gtacggccat cacggtcgta ttagtcattc tggccttcat cgcaattttt
    24241 aatgcctggg tctattacac cgaatccccc tggacgcgtg acgcgcgctt tagcgctgac
    24301 gtcgttgcga tcgcgccgga cgtttctgga ctcattaccc aggtgaatgt tcataaccag
    24361 ctggtgaaaa aaggacaggt tctgttcacc atcgaccagc cgcgctatca aaaggcgctt
    24421 gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa acgccaggag
    24481 gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat cgaccaggcc
    24541 aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac ccgcgatctg
    24601 gcaaaactgg atcttgaacg cacggtgatc cgcgcgccag cagatggctg ggtgaccaac
    24661 ctcaacgtct ataccggtga gtttattact cgtggatcaa cggcggttgc gctggtgaaa
    24721 cagaactctt tctatgtact ggcctatatg gaagaaacta agctggaagg ggtgcgccct
    24781 gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg gactgttgat
    24841 agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg gatggcgacc
    24901 atcgactcta accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg tattcgtctc
    24961 gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt ggtcactggc
    25021 aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca tcgcctgcgt
    25081 gagtttggtt aatcacgatg gatattttct ccattgctaa ccaacatatt cgctttgcgg
    25141 taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc cagctggaaa
    25201 cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc tttgctgcgg
    25261 gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc atcggcacat
    25321 ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca ccattattga
    25381 tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg ctggtacgaa
    25441 tagaaaactc gtatgcgtgg gggctggccg gttataccgc gctgatcatt gtgatcacca
    25501 ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc gagatcgtta
    25561 tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg atcaaacaag
    25621 aagtggatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg caactctgta
    25681 tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg cgacgcacca
    25741 cggcgctaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg gcgcgggcca
    25801 atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa tcctgcgaaa
    25861 cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc gaattttttg
    25921 acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg cgaagagtta
    25981 tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc gcggcggcaa
    26041 cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac gccaccgaag
    26101 aagagatcct gcaaggcgaa ccggaagtca aagtagagtc agccgaacgt catcatgcga
    26161 tggttaactt ctggcgaacc acactttcct gcattctggg aacgcttttc tggctgtgga
    26221 cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg tcactggcaa
    26281 tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctacgggacg ctggccgcgc
    26341 tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag agcatgttgc
    26401 tgctgtgtat tagcctggca gtgctgggat tcttcctcgg aatagaagta cagaaacggc
    26461 gactgggctc gatgggggcg ctggccagca ccataaatat tatcgtgctg gataacccga
    26521 tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc ggctgtgtgc
    26581 tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact ggacgtgtac
    26641 tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg gcacgtcgta
    26701 aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat aagttcccag
    26761 gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcccaccag cgcctgcgtg
    26821 atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg cgccgcacag
    26881 cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt ggtcagttgc
    26941 tggaagaact ggaaatctac caggaaaagc tatgcatctg gcaagcgcca ccgcaggtga
    27001 cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg ttgaccgata
    27061 gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt agcgatgcac
    27121 ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatac ctttgatttt
    27181 gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt tggtctggcg
    27241 aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga tgtcctgccg
    27301 ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg ttttggcgcg
    27361 ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg ttttaatgtt
    27421 cgtcattagc gcaaaaaaaa gcccccgaac cgggggcaat atcgtcggac aagacgatga
    27481 gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga gcttcagtaa
    27541 tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca cgcatatcca
    27601 ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga ttttgtgctt
    27661 gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa agtacgctta
    27721 atgcagcaac agtggttttg attttcatga tttttacctc gacataatct tttagctggg
    27781 cctttgtttc gtgacccata tcacaaaatc aagtatacac taa
//
LOCUS       DABSYT020000059        27127 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.059, whole genome shotgun sequence.
ACCESSION   DABSYT020000059 DABSYT020000000
VERSION     DABSYT020000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.059"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            15..1031
                     /locus_tag="ID725_RS14945"
                     /old_locus_tag="pgaptmp_002989"
     CDS             15..1031
                     /locus_tag="ID725_RS14945"
                     /old_locus_tag="pgaptmp_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGV7851533.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            1116..1538
                     /locus_tag="ID725_RS14950"
                     /old_locus_tag="pgaptmp_002990"
     CDS             1116..1538
                     /locus_tag="ID725_RS14950"
                     /old_locus_tag="pgaptmp_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGV7851534.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            1535..1738
                     /locus_tag="ID725_RS14955"
                     /old_locus_tag="pgaptmp_002991"
     CDS             1535..1738
                     /locus_tag="ID725_RS14955"
                     /old_locus_tag="pgaptmp_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851535.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(1742..2149)
                     /gene="cueR"
                     /locus_tag="ID725_RS14960"
                     /old_locus_tag="pgaptmp_002992"
     CDS             complement(1742..2149)
                     /gene="cueR"
                     /locus_tag="ID725_RS14960"
                     /old_locus_tag="pgaptmp_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGV7851536.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(2146..3321)
                     /locus_tag="ID725_RS14965"
                     /old_locus_tag="pgaptmp_002993"
     CDS             complement(2146..3321)
                     /locus_tag="ID725_RS14965"
                     /old_locus_tag="pgaptmp_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HGV7851537.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(3318..5480)
                     /locus_tag="ID725_RS14970"
                     /old_locus_tag="pgaptmp_002994"
     CDS             complement(3318..5480)
                     /locus_tag="ID725_RS14970"
                     /old_locus_tag="pgaptmp_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HGV7851538.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALLLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(5484..21211)
                     /locus_tag="ID725_RS14975"
                     /old_locus_tag="pgaptmp_002995"
                     /pseudo
     CDS             complement(5484..21211)
                     /locus_tag="ID725_RS14975"
                     /old_locus_tag="pgaptmp_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(21248..25633)
                     /locus_tag="ID725_RS14980"
                     /old_locus_tag="pgaptmp_002996"
     CDS             complement(21248..25633)
                     /locus_tag="ID725_RS14980"
                     /old_locus_tag="pgaptmp_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGV7851539.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMRDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            complement(25736..27091)
                     /locus_tag="ID725_RS14985"
                     /old_locus_tag="pgaptmp_002997"
     CDS             complement(25736..27091)
                     /locus_tag="ID725_RS14985"
                     /old_locus_tag="pgaptmp_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HGV7851540.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPGVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
BASE COUNT         5040 a         8008 c         7876 g         6203 t
ORIGIN      
        1 acaagagaaa atgaatgaaa actgtaaacg tagctttact ggcactcata atttcagcaa
       61 catccagccc tgttgtttta gctggtgata ccattgaagc ggcggcaaca gagctttcag
      121 ccattaactc tggcatgtcg caatcggaga ttgagcagaa gattacccgc tttttagaac
      181 gcacagacaa cagccccgct gcgtatacct atttgactga acatcactac atcccttctg
      241 aaacacctga taccactcag actcccactg tccagacaga tcctgacgca ggacaaaaaa
      301 ccgttgccgc tacaggtgat gtacagacaa ctgcccgtta tcagagcatg atcaacgccc
      361 gacagtctgc ggtaactgac gcccagcaaa cgcaaattac agagcaacag gcgcagatcg
      421 tagccacaca aaaaacgctc gccgcgactg gagatacgca aaataccgcg cattatcagg
      481 aaatgattaa tgccagactg gcggctcaaa atgaggctaa tcagcgcacc gccactgaac
      541 aagggcagaa aatgaatgcg ctgacaaccg atgtggcagt acaacagcaa aatgaaagga
      601 ctcaatacga taaacaaatg caaagtctgg cgcaggagtc tgcccaggca catgaacaaa
      661 ttgacagcct gtcacaagac gtaacccaaa cgcaccaaca gttaaccaac acccaaaaac
      721 gggttgcaga taacagccag caaattaaca cgctcaataa ccatttcagt tcgctaaaaa
      781 acgaagttga tgacaatcgt aaagaagcca atgcgggaac tgcatctgcc atcgctatcg
      841 cctcacaacc acaggttaaa accggtgacg tgatgatggt gtcagcggga gcgggaacct
      901 tcaacggtga atctgcggtg tctgtcggaa catcatttaa tgccggaacg catacggtac
      961 ttaaagccgg tatttctgcg gatacacaat ctgatttcgg cgcaggtgtc ggcgtgggat
     1021 attcgttcta atatttcaat cctcaatata aataagagca aggaagcttg ccgggttcac
     1081 ctcttcatta atttgtacat tatttaaggt taacaatgat gaatagctcc attaaatcgt
     1141 tttccctgct ggcggttata ttactggctg gctgtagttc acccacttcc cgcatcgcag
     1201 attgccaggc gcagggcgtc agtcatgaca cctgttacct cgcagaacag cagcgtcagg
     1261 cggctatttt aagtgcatcc gaggcacagg catttaaaaa tgcagaagcc gcacaacacg
     1321 cccaggcggc aaagaaagcc atttataaag gatttggcat gacctttaga atgagcagta
     1381 aaaactttgc ttatctcaat gattcattat gtgcaattga tgaagacaat aaagatgcca
     1441 ctgtttatca gtcaggtcta tataacgtca ttgtttatca tcacacagga aaagtcgcct
     1501 taatgaaaga aggccagttt gtgggttatt taaaatgaag gagcaaagga aaatacccct
     1561 gacgcatatt atgattatcg gtgcgtttat ttttgccttc ttgcaagtag tattattagc
     1621 ctccctggtt cacgctgtga atgttaacaa cgaaatccag gaaggcttat ttcagtcggg
     1681 gcgcattatg gtagaaagtt tgcagcatat tctttcggtg caaacgggga ttcactgatt
     1741 ttcaccccgc ccgatgatga cagcagccgg agagattttc gataatcggg cagtcggcgc
     1801 tgtcatcacc ggggcaggca ttcgccagcg ccagtagctg gttgcgcata gattgcagtt
     1861 cttcgatatg ccgttcaatc tccgccacct tctccagcgt gcgacgtttg acgtcggcac
     1921 tgtgacgctg cgggtcgtta aacagattca ccagctcgcc gctctcttcc aggttaaagc
     1981 ccacctggcg cgcctggcgc agtaaggtca attcgttgag atgctgctgc gtgtaggttc
     2041 gataaccatt ttcgctgcgc atcggcggcg tcaccagccc cttctcttca tagaagcgaa
     2101 tggctttgct ggtcaggccg gtaatttttg ctacatcgct aatgttcatc gttcgcgcaa
     2161 cgcctcctgt gctttgttaa agggtttcaa cagataatcc agcacgcttt ttttgccggt
     2221 gcggatatcg accgtcgcca ccataccggg gaatatcgga aactgctgtt tgcttttgtt
     2281 ttccagatgg ttgctgaaag tgcgaatgta cacccgatag taataaacat cgcgccgcac
     2341 ttcatcctgc acggtgtctg gtgatatcac cgccacttct cccggcaggc cgccgtagat
     2401 ggagtaatcg taggcagtta ttttcaccag cgatttttgc cccgggtgga taaacgccac
     2461 atcgcgcggg gagatttttg cctcaatcag cagttgctca tccagcggca cgatggtcat
     2521 cagtttacca ccgggggcga tgacgccgcc aaccgtggtg acatcaatat cctgaacaat
     2581 gcctcgcacc ggcgcggtaa agttgagcct ggtgagagag tcttcccgtc cacggatcac
     2641 cgaacgctgg gtttccactt ccgcattggc ttttgctaac tcttcgcgcg cctgaacgta
     2701 gtattgcgtg cgcacgtcgc tgagtttgtt ttccagttca ttggcctggc gttgcaggcg
     2761 cagtacttcg acgctgctcg ctgccccctg ttttaccagc ggctgggtca tcgccagttc
     2821 gcgattgacc aggctaatcc cctgcgtcag cccggtggtg gctttgttca acccgtcgcg
     2881 gcgagtgttg tacaacgccg tttccgactc gataagttcg gggaatttat tcagttcgtc
     2941 agggaagacc agcggtttat tgctcacttc cgcccgcagc cgcgccgagg ttgccagcgc
     3001 cgcgtacagc cgcgacatcg cttcctggac gtcggattcc gttttggtgc ggttaagctg
     3061 cgccagcggc tggccctgtt cgacaatatc gccaactttc acgtcgagat gatacagaat
     3121 tccgccttcc agcgactgca ccacctgttc gcgcccggaa ggttctaccg tgccgctgcc
     3181 ggtggtgact tcatcaagct ggaagtacca cgcccagccg agaaaacaga gcagcatcac
     3241 agcggtaacg cgtaccgcca gcgccgaacg cggcaggcgg ggttcagtca gaccacggtt
     3301 atattgcagt tgtccgttca tgccgcagac cccttattct tctcctgcaa caccacccgt
     3361 cgcgctgtgg gctcgccctg ttcacgcaaa atggcctctt tcgcgccgtc catcacaatc
     3421 cgcccctgat ccatcacgat tatcctatcc accagttgca gcatcgccgt gcggtgagtg
     3481 gcgataatca gcgtgcgatg cccaatccac tgtttcagtt gttcgattaa atacgcttct
     3541 gccatttcgt ccagtgaggc gctgggttcg tcgagcagga cgatattcgg ctgagagatg
     3601 agtagccgcg ccagcagcag cgtttgccgc tgaccaccag agaggccaaa tcccccttcc
     3661 tgaatcatgt agttgaggcc gtttttctgc ttctgcacaa agggcagcgc tccgctgagg
     3721 gttaacgcgc gttcgatatc ttcatcgctg gcttccggca tccccatggt gaggttttcc
     3781 cgcagcgaac caaagaacag tcgcccggtc tggctgagta gccccatgtc tcgccgcaga
     3841 tctgccggat caagctgccc gatactgatg ttatccagca acacctgccc ctgctgggcg
     3901 atacgcattc cggcgagcat ttgcagtagc gtcgatttac ccgcgccgtt gcgcccgaga
     3961 atggcgattt tctcaccagc ctgaatgttg agtttgccaa tcgctacatc ggcaatcttc
     4021 tcttcttcgt cgtaataaaa caccgcgttg ctgaactgat aattaccgtg cagcaccgct
     4081 ttatgcacca gtttgccgtg ttccggctga tccaccgggc gcttcatcag ttcatcaagg
     4141 ccgttacgtg ctactttcgc ctgctgccag cgcgagagca cgccggatat ctgcgacagc
     4201 ggcgcaatgg tgcgcgaagc caggatcgtc gtcccgacca gcgcgccggt ggtcatgtcg
     4261 ccgttcatca ccataaagca gcccaccagc agcaccacca cgtagacgat ggactgcact
     4321 tcctgggtcc aggtgagcag cagcccggtg agaaaacgtt gcttcatgct gatggacgag
     4381 gccacgtcgt tggtgtggtt ccactgattc tgaaaacgct gttcggcgcg cagcagttta
     4441 atatcttcaa tcgactgcac ggcttccacc agcgtagcgt tgcgtaccgc tgactcgcgc
     4501 attccttcgt tcgccagccg cgccagcggg cgttgcacca gcaggccggg gatcaccagc
     4561 aacagcagcg ccagcaaaac caccagcacc agccagccgc cgatcatcca caaaataaag
     4621 acaaacagca ggaagaatgg caggtccgcc accgcgccga tggtggtgga cgtaataagc
     4681 tcccgcactg attccagttc gcggatctgc gagataaacg atccggtgga tttcgatcgc
     4741 acgttgtttt tcagccgtag cgcatgacca aaaacgcgat ccgaaatacg caggtctgcc
     4801 cgctttccga tcacatcaga tagatgcgta cgcaccatgc gcatacagaa ctcaaacagg
     4861 atcgccatca tcaccccggc gaaaagcacc catagcgtcg ggtacgattg cgccggaacc
     4921 acccgatcgt aaacctgcat cgagaaaatc atcgccgcca gcgccagcac gttcgccacc
     4981 agtgacgcca gcataatatc gccgtagcgc cgccagtctt ttagcgaaag actccagaac
     5041 cagttcgcct gatacgggcg aatatagtca tccacccgcg cgtcagggat ggcgctgaga
     5101 gggcgcagca gcgccagttc gacgatttta tcttcaatat ccgcgacgtt aagtgtagtt
     5161 tccagccctt cgtcaccgct gaactgaacg ctgatattgt catgattgtc gcgacgggtg
     5221 atcacgccaa tctgctgatt atcaaacacc gcgatcagcg gcagacgcca ggggttaagc
     5281 gaatcggcgg aaaactccac caaccgcaac cccatcccta actgccgcgc catgtcggtg
     5341 agtaactcct cccgcttact gtcgcgctcc cagttgacgg tcgcgcgcac attctcctga
     5401 gaaaaatcga gccgataata gcgcgccacg gcgatcatcg cctccagcca ggcttccacg
     5461 gactgggcat tttttttcat tatctacacc accatcaact gatgattcgc cagcaaggtg
     5521 gcgaggtcgg tgtgcacgcc cgtcagcgtg accacgttag ttgtcgcaaa agtgccgccc
     5581 gtgccgtcgc ggtcgatctg cacgatggtg tcgctgccgc tctgggtgac tttgacaaag
     5641 tcaccgatgt ttccggcctg cgcatccagc gttgccacgc cgttgacgta gctggctttg
     5701 ccgttgccgg tgtagccgct gccctgtaac agttcacgaa tatcaatgcg atcggtgtcg
     5761 gcggtgcctt cccatgtccc taccgtaaag ccattcacca cgtctgagcc gttgccgcca
     5821 gtggcgtcgc tggcgttgag cagtttatag agcaaggtgt cgtggccgcc gttgccgatg
     5881 ttgagcgtgt cgttgccgcc gcgcccttcc agttggttat cgccgctgct gccggtcaga
     5941 atgtcgttaa aattcgagcc ggaaatgcct tcgatattgg taaatgtcga ggtgttaaaa
     6001 ccggtggcct gcgcggcagt gctgcttaag ttagccgtca cgcccaccgt cgaaagccgg
     6061 aaatcaacca cgtccatccc gccgttcgcc agccacgttc cggtgccgga gctgtacacc
     6121 cagccgccgg aaccgtcgta agtgtcggtg cctgcggtgg cgaagaaggt gtcgttatag
     6181 ccggtgccga cgaacttgcc gttgttgctc gcggtacccg cttcagcgct gagatcgtaa
     6241 gcgtgagtgg catcggtggc ctgtaaaccg ccccagctgg tgttgaccgt ctggtcgata
     6301 ttgctggcgg tggtgccggt ggatttgatc agcaccgcgt tgtaggtttc tccggcattc
     6361 agcccgtaga agttgaccag cgccgaggtg tcgttaaccc ccatacccga ctgcgggttg
     6421 atgatttgcg tggcgaccag cactcccgcc gagttgtaca gcttcacggt attgccgtaa
     6481 tagacgttaa tgccgttggg atcggtgatg cgcaggtgta gcgaagtgcc gtagctcacc
     6541 gtgttggtgt tgcggctcag gaacacgctg cccgactgtt tggacaccag cacatccacc
     6601 gcgccgtccc agtcgtagtc cattgccgcc acgccgctgg tggtaccgct ctggctgccg
     6661 ccgagggccg attgcgtcca gttgctggcg ttgctgacgt tagtataaag atacgactgc
     6721 ccggtctggg cgatacgggc gatatccatc aggccgtcgt ggttccagtc caccgccagg
     6781 ctaaactggc tggcgtaggt ggttgccgtt gcgcccaccg ccaccgggct acccagtacg
     6841 ccgttggtgt tgaataacag cgagccgtag ttagctcttc cctggctggc gggcaggaag
     6901 agatccatat cgccgtcacc atcgaaatcg gcccaggtca tggaaacgct gctgctcata
     6961 gcgccactgc ccgccccgct caggaaggta ttggtggtgt tctgccccca gacaaacgtc
     7021 ccattcccct ggttgatgag cgaagacagc gtgtaatagt tgttcaggtt ataggtgtgc
     7081 tgaaccaggt cgatcctgcc gtcattgttc agatcgacgc cagaaccttc gttgagcgaa
     7141 agataccccg tcaccgcccc gcccaccgtg gcgctaccgc cgctgttcga cgtggtggag
     7201 ttgcctacca gcgtgcctgc gttgttccac aggaaggtgt tggagtccgg cccgccagcg
     7261 tcaccaatcc agaagtcgag atagccgtcg ccctctttat caaatgccac aatcgagccg
     7321 taccacgtca gggtgccgac ggtgacctga atagcgctgg aaaaggtgcc atcgccgtta
     7381 ttggtaaagc cgttaatata gcccgtaccg tagtcatcac gggtgataaa cagatcgccc
     7441 gtgccgttac ggtcgtaatc ggcaatggag ctggtggcgt agttgttgcc gctctgatac
     7501 agcgtcagcg cggtcttcga ccaggtgagt gggtcagtcg attgcattac ctgctggttt
     7561 gccagcaccg tccacatccc gtgcgagtcg aggccgtagg tcagccccca ggcggtggtt
     7621 ttgctggcgg tagtccaggt cggtgtgtaa tcaatcgccg cgttaaccgt caccgtgccg
     7681 ttgctaatat tggcgttatt gccgttaccc gccgaacttt ttacctgcgc agtaacggta
     7741 taggcggtcg cggaaactgt cagcgcatcg gtatccggca actgtacata ccaggtgttg
     7801 tgcgccggat cgaccactac cgcgccgccc ggttcagagg tgtaggtttt gccgttgatc
     7861 accacttcaa catactgccc actggcaagc gcggcggtga tcaccccgct atagtatcgc
     7921 gcgtggtctg gctggtgatt tgcgccagcg tggtcggaat agaggtatcg accgtcagga
     7981 caaaatcact ggaggaggtg atattccccg ccaaatccac cactcgcgcg ctgtaggtat
     8041 aggcaccgct caccagcccg ctgtcgctgt aggtccagtt cagagcgccg accatcgtca
     8101 ccgcccctaa cagcagcccg ttacggtaga ggtaaaccac ttcaccgctg gcaagcggcg
     8161 cggaaagtac accgttcagc agcggcgtag tgtcgtcggt ggcctgcgaa ctgcttaatg
     8221 tcccctgccg ctgcccgaca tcatcggtat acgacacaat cgttggtgtg gcggtggggt
     8281 taatcgtatc gaccaccacc gttttcgaca ccaccgggcc ggtgttcccc gccagctcca
     8341 gcacccgcac ctgataaacg tagctgccgt cggtcagcgt gcggctgtcg gtgtaagtcc
     8401 actgtgtacc gttggtggtg agcgttgtcc aggtcgcgcc gctatcaagg ctaatctgcg
     8461 ccacttcgcc gctggcaagc cccgccccca gcgaaccggt cagcgtgagc atggtgtcgc
     8521 tggtgataaa gtcagttgcc gaactccccg tgtcggtgct gatgctggcg atggtgagca
     8581 cctgcgccgg gttaacggta tcgatcagcg ccgactggct gctcgttgcg ccaacgttgc
     8641 ccgccagatc gaccacccgc acctgccagg tggtggtgcc gttggtgagg gttcgtccgt
     8701 caacgtaact ccagttcagg ctgtctgcgg cgacggtcac gttcacccag gttgcgccgt
     8761 tgtcggtact gatttgcgcg aactcattag cgccgagcgc cgcgcccaat acgccgcgca
     8821 cggtaagcgt ggtgtcgctg gtaataaaat cgctgctgct ggttccggtg tcatcgctga
     8881 taccggtaat ggtgatactt ttcgccgctt ccgggctggt ggtgtcgacc accacgtttt
     8941 gcgttgcggt ctgcccaacg ttccccgcca gatccaccac ccgcaccgtg tagttccagg
     9001 tgccgtcagt aagcgtgtga ccatcggcat agctccagct ggtgccaacg acggttaacg
     9061 tagtccaggt gacgccgccg tcgaggctga tttgcgcgaa ctcaccggca gaaagcgtcg
     9121 cccccagcgt gccgctcacc gcaagcgtcg tgtcgctggt gacaaaatcg ttagtgatca
     9181 agcccatatc ggtggtgatc gcgctgatgg cgatggtttt caccgcggga tctggcgcgg
     9241 tagtgtcgat caccacattc tggctgtcgg tcgcgccaac gttgcccgct gcgtcaatca
     9301 cccgcacctg atagagatag ttgccatcgg tcagcgtgcg gctgtcgtta tagcgccagg
     9361 tcgtgccggt aacggtaagc gtggtccagg ttgtgccgcc gtcgatactt atttgcgccg
     9421 tctcgttact ggcaagcgcg gcggtgagcg agccgttgac cactaccgga ctgacgctgg
     9481 tgataaagtc gctggcgcta aggccagtgt cggcttgcag ggaatcgata ttaatgccca
     9541 tcgccggagc gctgagatcg acggtaatgg tgtgattact gctaccggtg ttgccaatgg
     9601 cattgctcac cgaggcgtta atcacgtagg cgttgccgtc cgccagcgcg gtgacatcgg
     9661 cgctgccgag ggtgtagctc cagctaccgc ccgtctgcac cgtggtggtg taggttttgc
     9721 cgtttaaggt cagggtcagc gtctgcccga cgggcgcggt ggtggtgccg ctgatcacca
     9781 gcgatgagcc gtgttccgct gcattcacca catcgtcgcc cgcaaaggtg ttgatggtga
     9841 cagtaggtac atcgccgttc agcgtcacgc cacggctggc gctgccgggg tttccggcct
     9901 gatcgctaac cgtcgcgcta atggtgtagc tgccgtcgct taatccggca aactgctgcg
     9961 ccggaataaa caccgaccag cttccgccgc tgccaaccgt tgcgctccag gtttgcccgt
    10021 taaaggtaac ggtcaccgtc tggcccactt cggcggtggt ggtgccgcta atggtctgcc
    10081 cggcaagctg ctcggcgttg ttgataatat cgtcacccgc caccgtggcg atggtgaccg
    10141 ttggcgcaac ggtatcgacg gtcagggcgt gcgtggcctg tccggggtta ccggcgcgat
    10201 cgttcacgct ggcggtaatg gtctgcgcgc cctgcccgag cgcggccaga tccaccgcag
    10261 gcacgttcag cgcccaggta ccattggcgg caaccgtggc ggtataggtt ttgccgccaa
    10321 aggtgacggt gaccgtctgc cccacttccg cgctggtcga gccgttaagg gttaacggct
    10381 gctgctgttc ggcggcgttg aggcggtcgt ccgtcgaaac ggtgttaatg ctgataaccg
    10441 gcgcggtgag atccaccgcc acgctgcgcg aggccgtggc gctgtttccg gcactgtcct
    10501 gagcggaggc gctaaccgtg tagctggtgc catcactgag tgccgcaaca tccgccgcag
    10561 gcacgttcac gctccagctc ccgttgctct gaatggtggc gctgtagcct ttgccgttaa
    10621 gcaacacggt caccactgta cctgtcgcga aattagtgcc agtgccgctc agggtcagcg
    10681 cactgcccgc ttcggcggcg ttaataaggt tatcgccgct gatagtgctg accgagagcg
    10741 acactgccgc cgtgttcacc tgtacgttgt gcgtctgcgt gctgctgttg cctgcgctat
    10801 cggtaatggt ggcgctgatg gtcaccgtgc catccgcaag gccggaaatc acgctggcag
    10861 gcacgccgac gctccagttg ccgctggcat cggtgctggt gacatactgc tgcccggcga
    10921 tggtcaccac caggcggtcg cccgccaccg cgcccgtggc ggtgccgctg ataatctgcg
    10981 cctgaatgtg ttcgacgttg ttaatcacat catcgcccgc cacggtgtta aaactgatca
    11041 ccggcgcagt ggtatcgacg gtcacgcctt tgctggcgct gccaaggtta cccgccagat
    11101 cgctgaccgt ggcggtcagg gtgtagccgt tattagcaag tgcggttaag tcgctggcgg
    11161 gcagcgttaa actccagctg ccgtctgtct gcaccgtggt ctggtagtta gtgccgttaa
    11221 gcgtgacggt gagcgtctgc ccggtttgcg cggtgctggt gccggacacc gttacgcccg
    11281 ccgccgcttc gctggcgttg atgatgttat cgctggcaat ggtgttgatg gtcaccgttg
    11341 gcgcggcgct atcgacgctg tattcgtgcg ttgctgtggc gctgttgccg ttgacgttag
    11401 tcacccgcac ctgggcgctg gcatcgccat cacgcagggc cgccaaatcc gccgccggaa
    11461 cggtggtgct ccagctaccg tttgccgcca ccgtggtggt gtaggttttg ccgccaaagg
    11521 tgacggtgac ggtttgcccc gcttcgacgc cggacgtgct gccggagagc gttaacgccg
    11581 cgcctttttc ggcggcgtta atcgcatcat cgctggtgat gctgttcatg ctgatggcga
    11641 cggcggtgag atcaacagtc agcggatggg taatgctggt ttgcgttccg gcactgttcg
    11701 ccccgctaac ggtaatattc agcgttcccg caggccagtt actgacatcc gtcgcaggaa
    11761 cgccgacgct ccagctaccg ttcgccaaca ccgtggcggc gtaagtttgg ttgttaatca
    11821 ccaccgtcaa cgccgtgcct gcggcaaagc cggagctgct gccggtgatc acctgcgcct
    11881 gagcgtgttc gatgatattg accacatcat cacccgcgac ggtatccacc cgcaggccag
    11941 gcagactggc atcgatggtg atatcgcgcg tgccgctacc ggtgttgcca acggcgttgg
    12001 tcaccgaggc attaaccgtt aactcaccgt tacccagagc ctgccagtcg gcggcaggta
    12061 cgctcacatt ccagcttaaa tcagcctgga cggtggcggt gtaagtttta ccgcccagtt
    12121 caacggtgac ggtgctgccc gccgccgcac cggttacctg gccgctgata gtttgcgcgc
    12181 tgcctgcctc tgaagcgttg ataatatcgt ccgtcgccac cggattaatg gtgatgcccg
    12241 gcagcgcggt attaacctgc acgttatggc tggcggtgct gctgttaccc tgagcattgg
    12301 tgacgctcgc cgtcacgctg taggtcgctt cgcccagcgc actgaccgcc gaggcaggca
    12361 cggtaacact ccagttgccg gaggcatccg tggtggcgct gtagttttgt ccgttcaggg
    12421 tgacggtgat cgccgtaccc gcaggctgat cgctggtgcc agaaagcgcc agatcagatc
    12481 ctttttccgt cgcgttgatc acatcatcgc ccgcgatggt gttgatgcta atcactggcg
    12541 cgctgaggct gacggtcacg gtgcggctgg cgtcgtcgct gttgcctgcc cgatcgctga
    12601 cgctggcggt gatggtctgc gcaccgctac ccagcgccgt aacatctgcc gccggaacgc
    12661 caacgctcca gttgccggag gcatccaggg tggtggtgta ggttttgccg ttgagcacaa
    12721 cgctcaccac atcgcccgct tccccgccgc tactggtgcc ggagatcacc agcgcctgcg
    12781 catgttcggc atcgttgatg atgtcatcgc ccgccacggt gttaatggtg agaaccggcg
    12841 cggtggtgtc taccgccaga ttatggctgg cgctgccggg gttgcccgct ttgtcattga
    12901 ccgtggcgct gacggtgtaa ttgcttgcgt tgagcgcgcc cagggctgaa gtgggaacgc
    12961 tgacgctcca gctaccgtcc gcctgcacag tgccggtgta ggttgcgccg ttaagggtga
    13021 cggtcaccgt ctgcccggtt tcggcggtgc tggtgccgct gatggtgagc gggcttccgg
    13081 cttctgcggc gttaaggata tcgtcgctgg cgatggtgtt aatggtgagc gtcggcgcgc
    13141 tggcgtcaac gctatacgca tgggtcgccg aggcactgtt accgctcgcg ctgctaacgc
    13201 tggcctgcac attcgccgcg ccctctggca gagttgccag atctgccgcc ggaacgttta
    13261 cactccagct cccgttggcg gcgacgcttg cagtgtaggt tttaccgcca aaagtgacgg
    13321 tgacggtttg cccgctctca atcccgctgg tactgccgga aagagtcaga ttggtgcctt
    13381 tttctgcggc gttaatcacg tcgtcgctgg caacggtgtt gatgctgatt gccaccgccg
    13441 ttaaatcgac ggtgaacgga tggctgacgc tgactgggtt attggcgctg ctttgcccgt
    13501 ctacctccac ggttaacggc cccgcaggcc aggcgctgac gtttgccgcc ggaatgccaa
    13561 cgctccagct tccgtccgcc tgcaccgtgg cggaatacgc cacactgttg atggtaaccg
    13621 tcagcacagc acctgcattc aggccgctac tgccgccggt gatcaccagc gcctgcccgt
    13681 gctcgatgct gttgacgata tcatcgcccg ccacggtatc tacgcgcagc cccggcaggt
    13741 tggcatcaat ggtgatatcc cgcgtgccgc tcccggtgtt gccattagcg ttggtgaccg
    13801 aggcggtaat ggtcaaatca ccattgccca acgcctggag atcggctgtc ggaacgctga
    13861 cgctccagct taagttgctc tgtaccgtag cggtgtaagt gttcccgccc agcgtcacgg
    13921 tgaccgtatc gccagcggcg gcgcgagtca ccaccccgct gatggtttga tcggcccccg
    13981 cttcggcggc gttgataata tcgtcgcctg ccacggtgtt gatggtgacg tccggcaggc
    14041 cgctgtcgac cagtaaattc gcctggctgc tggcggtgtt gcctgctgca ctggtggcgc
    14101 tggcgctgac cgtatagttg gcctgaccta atgccgccag atcgctcacc ggcacggtta
    14161 aggtccagtt gcccgatgcg tcggtggtgg cgctgtagtt gtgaccgttg agcgtgacgg
    14221 tgacctgcgc gcccgtcgcc agccccgtac tgccgccgct gatggtcaac ggctggcctt
    14281 tttcatcggc gttcaggatg ttatcgccgc tgatggcgtt aaaggtaatg gtcggcagcc
    14341 cggtattgac cgtcacctga tgggtagcgc tgccgctgtt tcctccggca tcggtgacgc
    14401 tggcattgat ggtcaccgtg ccattcgcca gtgccgagac gacgcttgcc ggaacgccga
    14461 cgctccagtt gccgctggca tccagcaccg tggtaaaggt atttgtgccg atagtcaccg
    14521 tcacggtgct accggttgcc gctccagtgg cggagccgct gatgatctgc gcctgggcgt
    14581 gttccgtcgc gttgatcaca tcatcgcctg ccaccgtgtt gatggtgacg accggaacgg
    14641 acgtatccac cgtcaggttg tgattaaccg aggccgggtt tccggctttg tcgctcaccg
    14701 ccgcgttcac ggtgtagttg ctggcggtca gggtgcttaa gtcggctgac ggtacgctga
    14761 cgctccagct gccgtccgtc tgtacggtgc cggtgtagtt tgtgccattg agcgtgacgg
    14821 tcaccgtctg ccccgcttcc gccgtgctgc tgccggtgat ggtcagagcc gctccggctt
    14881 cggcggcgtt aagaatatca tcgcccgcaa tggtattaat ggtcaccgtt ggcgcgctgg
    14941 catcaacgct gtaagcgtgg gtcgtggtgg cgctgttgcc gttaacattg ctgacgctgg
    15001 cctgtgcgct ggcatcgcca tcacgcagag ccgccatatc tgccgccgga accgaggtgc
    15061 tccaggaacc attcgcagcc accgtggcgc tgtaagtttt gccgccaaag gtgacggtaa
    15121 ccgtttgtcc ggcttcaacg ccagaggtgc tgccggagag cgttaacgcc gcgccttttt
    15181 cggcagcgtt gatcacatca tcggcggtaa tggcgttgat gctcaccgcc accgccgaga
    15241 gatcgaccgt caccggatgc gtaacgctta ccggatttcc ggcagaggtg ctaccgctcg
    15301 ccgcaatcgt caccgatccc gcaggccagg cgctgacatc caccgccgga acgccgacgc
    15361 tccaggtgcc atccgccagc accgccgcaa cataggtttg cccgttaatg gtcagcgtga
    15421 cgttgctgcc cgccgccaga ccggagctgc tgccggtaat caccagcgcc tgcccgtgct
    15481 cgataatatt aaccacatca tcgcccgcca cggtgtcgac ccgcagaccg gggagattcg
    15541 cgtctatggt gatttcgcgc gtgccgttac cagtattgcc tacgctgttc gtcaccgaag
    15601 ccgaaatggt cagttcgccg ttgcccaact cctgtagcgc ggaggccgga acatcgacgc
    15661 tccagcttaa attcgcctgc accgtggcag tgtaagtcgc cccgccgagc gtgacggtca
    15721 ccgtgtcgcc tgccgccgca cccgtcacct gcccgctgat ggtctgttcc acgcccgctt
    15781 cggcggcgtt aataatatcg tccgttgcca ccacgttaat ggtgacgccc ggcagcgcgg
    15841 tattaacctg tacgttatgg ctggcggaac cgctgttgcc atcggcgtca gtggcggctg
    15901 cggtcaccgt gtaggtggct tcaccgaggg cgctcacccg tgacgccgga acggtcacgc
    15961 tccagttacc ggaagcatcg gcagtggtgg tgtaattttg cccgttgagc gtaacggtaa
    16021 tctgcgtgcc cgcaggctgg ttgctggtgc cggtaatcgc cagatccgcg cctttctccg
    16081 cggcgttgat gatgtcatcg acggcaatgg tgttaatggc gagcaccgga gcgccgagcg
    16141 ccacggtgac agtgtgcgag gccgtgccgc tgttgcctgc ggagtcggtg accgtagcgg
    16201 taatggtcac atcgccctgc gccagcgcgg aaatcacgct ggcaggcacg ccgatgctcc
    16261 agttgccgtt ggcgtccagc acggtggtat aggtggtcgt gccaatcgtc acggaaaccg
    16321 tattaccggt agtcgcgccc gttgccgagc cgctgatgat ctgcgcctgt ccgtgttccg
    16381 tggcgttaat gatgtcatcg cccgccaccg tgttgatggt gacgaccgga gcggcaaggt
    16441 ccaccgtcag attatgcgtt gccgaagccg aatttctggc tttgtcgctg accgaggcgt
    16501 taacggtata cgagctggcg gtaaggctag ctaaatcgcc ggttggtacg ctgacgctcc
    16561 agctcccgtc tgcctgcaca ttgccgctgt aattaacgcc gtttagcgtt acggtcaccg
    16621 tctgcccggc ttccgccgtg ctggtgccgc tgatggtcag cgccgatccc gcttcggcgg
    16681 cgttaagaat atcgtccgtg gcgatggtgt tgatggtaac ggagggcgca gtggcatcaa
    16741 cgctgtaagc atgcgttgcc tgggtgctgt tccctgcgac attgctgacg ctggcctgga
    16801 cgtttgctgc gccgtctggc aaggttgcaa catcgacggc agggatcgtc agcccccagg
    16861 tattatccgc ggcaaccgtg gtggtgtagc ttttgccgcc gaagataacg gtgatggttt
    16921 gccccgcttc cacgccggag gtggtgccgg agagctgcaa atcagagcct ttctcggcgg
    16981 cgttaatcac atcgtcagtg gaaagggtgt tgatggtgat cgccacggca gcgagatcga
    17041 ccgtgaccgg atggctaatg ctggttgtgg ttccggcggt gttggtgcca gagacggcaa
    17101 tattgaccgt ccccgcaggc cagtttgtga catctgccac cggaacacca acgctccagc
    17161 taccatccgc taaaaccgtt gcgccgtagg tgacgctatt gataaccacc gtcaacgccg
    17221 cgcccgccgc cagtccgcta ctgctaccgg tgatcaccaa cgcctgagtg tgctcgatgc
    17281 tgttaatcac atcatcgcct gccacggtat cgacgcgcag acctggcagg ttggcatcta
    17341 tcacgatatc gtgcgtcgcg gtgccggtat tatttgcgga attggtcaac gaggcgctga
    17401 tggtcagttc accgttaccc agcgcctgca aaacgttcgc cggtacgctg acgctccagc
    17461 ttaaatctga ctgcacggtg gcgttatatt gattgccgcc aatggtgaca atcaccgtat
    17521 tcccggcaac cgccgttccc gtgacctgac cgctgatggt ctgcgccaca acgatttcag
    17581 cggcgttgat aatgtcatcg ccggagacgg tgttaatggt cagcgtcggg ccgctaagat
    17641 caacggtcac atcgtgagtc gtgctgtcgc tgttgcccgc gcgatcgctc aggctggcgg
    17701 tgatggtctg gctaccggtc gccagcgccg tgacatccgc cgccggaatg cccacgctcc
    17761 agttaccgga ggcatccagc gtggtggtgt agtttttacc gttcagcgtg acggttacca
    17821 catcacccgc cgccgcgccc gtactggtgc cgctgaccac caacgcctga tcatgttcga
    17881 tggcgttgat aatgtcatcg cccgcgacgg tgttaatggt gagatccggc gcagtgatat
    17941 ctaccaccag cgcgtggtcg gcgcttgccg ggttacccgc tttgtcgctc accgtggcgg
    18001 tcacggtgta actgctggcg gttaatccgc ttaaatccgc tgccggaaca ttcacgctcc
    18061 aggtgccatc cgccagaacg gtggtctggt acgtgttgtt attgagcgtg acggtgagcg
    18121 tctgcccggc ctgcgccgtg gtagtgccgc tgatggtgat ccccgcgcca gcttcgctca
    18181 cgttaaggat atcgtcgctg gcgatggtgt tgatggtcac aagcggcgcg gtggcatcaa
    18241 cgctatacgc gcgatcggcc tgggcgctgt tgccgttaat attactgaca ctcgcctgca
    18301 cgttgcccgc gccgtccggt agcgcagcga gatcggcagg cggcacattc accgtccagc
    18361 taccgttagc ttccactgtg gtggtgtaat ttttgccgcc aaaggtgacg gtcaccgtct
    18421 gcccggcttc gacaccgctg gtgctgccgc ttacgaccag cgtttcgcct ttttcgacgg
    18481 cgttgatcac atcatcacca gagagagtgt taatggtgat ggcgacccca gccagatcga
    18541 cggtaaccgg atgggtaatg gtggaggttg ttccggcact gttggtgccg cttaccgtga
    18601 tattcaccgt acccgctggc cagttgctga cgtctaccgc cggaacaccg aggctccacg
    18661 tgccgtcggc taataccgtc gcgccataag taacgttgtt aatctcgacc gttaacgccg
    18721 ttcccgccgt taatccgctg ctgctgccgg tgatcaccag cgcctgattg tgctcaatgc
    18781 tgttaataac atcatcgccc gccacggtat cgacccgcag gccaggcaga ttggcgtcga
    18841 tcgtgatatc gcgcgagccg ctgccggtgt tgccgacgcc attggtgacc gaggcattaa
    18901 ccgtcaggtc gccgttgccc agcgcctgaa tatccgccgc cggaacgtcc acactccagc
    18961 ttaaattaga ctgcaccgtg gcggtgtagg tgttgccgcc cagcgtaacg gtaaccgtat
    19021 ccccctgcgc tgcgcccgtc acctgcccgc tgatggtttg cgcatttccc gattcggcag
    19081 cgttaataat atcgtcagtc gccaccgcgt taatggtaac ggcaggcaag gcgctgttga
    19141 ccagcacatt atgactggcg gaattactgt tgcctgccgt atcagtgacg tttgccgtta
    19201 ccgtgtagtt ggcttcaccc aatgcgctaa ccgctgacgc tggcaccgtg gcgctccagt
    19261 tgccgttgct atcggtagta gcggtgtaat tttgcccgtt cagcgtcacc gtaatggtgg
    19321 tgcccgcagg ctgattactg gtgccggtga tttgcaggtc cgcgcctttt tccgtggctt
    19381 taatcacgtc atcggtggcg atggtgttga taccaatggt tggcgcggcg agattcacgg
    19441 tgaccgtgcg gctggcgtca tcgctgttgc ctgccgcgtc ggtaattgcc gcagtgatag
    19501 tttgcgggcc gctgccaagc gcagtgacgt cagccgccgg aacgccgacg ctccagttgc
    19561 cggaagcgtc cagcatggtg gtgtaggttt tactgtttag tgtgacggtg atgacatcac
    19621 ccgcttcgcc gccagtgctg gagccggaga tcaccagcgc ctgtccgtgt tcggcggcgt
    19681 taataatgtc atcgccggag acggtgttga tggtcagcac cggcacggtg agatccaccg
    19741 ccagcccgtg gttagcggac gccgggttac ccgctttatc gcttaccgag gcactaacgg
    19801 tgtactggct ggcggtcaga ttgctgagat ccgccgtcgg taagctgacg ctccagctgc
    19861 cgtccgcctg gacggagccg ctgtaagtca caccattaag cgtgacggtt accgtctgcc
    19921 ccgcttcggc ggtgctgcta ccgctgatgg ttaacggatt gcccgcctcg gcagcgttca
    19981 gaatatcgtc ggtggcgatg gtgttaatgg caagcgtcgg ggccgtggca tcgacgctgt
    20041 aggcgtgggt tgccgaagcc gtgttgccgt taatagtgct gacgctggcc tgcacggtgg
    20101 cgtcgccgtc gcgtaacacg ctgagatcgg cggcgggtac ggtggttgtc cagctaccat
    20161 cgcccgctac ggtagcggtg taggttttcc cgccaaaggt aacggtgacc gtttgcccca
    20221 cttccacgcc ggaggtgctg ccggaaaggg ttaaatctgc ccctttttcg gcggcgttaa
    20281 tcacatcgtc gccggaaacg gtgttaatgg aaatcgccac cgccgccaga tcgacggtga
    20341 ccggatgggt gatggtggat gttgttccgg ctgtgttagt gccacttacc gtgatattca
    20401 ccgtacccgc aggccagtta ctcacgtctg ccgccggaac accaacgctc catgtgccat
    20461 cggctaatac tgttgcagcg taagtgaccg tgttaatcac caccgtcagc gccgcgcccg
    20521 ccgccagccc gctgctgcta ccagttatca ccagcgcctg agcgtgctcg atgctattga
    20581 ccacatcatc gcccgccacg gtgtcaacgc gcagacctgg caggttggcg tcgatagtaa
    20641 tatctcgcga accgctgcca gtattgccaa cgccgttggt caccgaagcg ttaaccgtca
    20701 gattgccatt gccgatggcc tgaatatccg ccgccggaac gtccacgctc cagcttaaat
    20761 tcccctgcac ggtagcggtg taagttttcc cgccaagcgt tacggtaacc gtatcgcccg
    20821 ccgccgcccc cgtcacctgg ccgctgatgg tttgcgcatt gcccgattcg gcggcgttaa
    20881 taatatcgtc ggtcgcaact gggttaatgg tgacgccagg cagcgcggta ttgacctgca
    20941 cgttatggct ggcggaattg ctgttgcctg cgctgtcggt gacgttcgcc gtcaccgtgt
    21001 agctggcttc gcccagcgca cctactgcgg aagctggcac cgtagtgctc cagttgcctg
    21061 cagcgtccgt cgtcgcggtg taattttgcc cgttaagcgc cagcgtaatc gtggtgcctg
    21121 ctggctgatt actggtgccg gaaatttgca gatccgcgcc tttttcgctg gcgttaatta
    21181 tgtcatcggt ggcgatggtg ttgatgtcaa tggttggtgc ggtgaggtta acggtgaccg
    21241 tgtgtgtcta attgtcgctg ttgcctgccg catcggtaac cgtggcggtg acagtttgcg
    21301 ggccgctgcc aagcgccgtg acatccgccg ccggaacgcc gacgctccag ttgccggagg
    21361 cgtccagggt ggtggtgtag gttttactgt ttagcgtgac ggtgacgaca tcccccgctt
    21421 ctccgccggt gctggaaccg gaaatcacca gcgcctgccc gtgttcggtg gcgttaataa
    21481 tgtcatcgcc cgcaatggtg ttgatggtca gcaccggaac ggtgagatcc accgccagat
    21541 tatgcgtggt gctcgccgga ttgcccgctt tgtcattgac cgtggcggtc agggtgtggc
    21601 tgccatcggt caatgcctca agatctgccg ccggaatatt gatgctccag ctgccgtccg
    21661 cctgcaccgt tgcctgatag gtctgcacgg tcgggctgtt aagcgttatc gtaacaatct
    21721 gccccgcttc ggcggtggta ctgccgctca ccgtcacgcc cgcatcggct tcgctggcgt
    21781 tgacgatatt gtcgctggcg acggtattga taatgatggt tggcgcgctg ctgtcgacgc
    21841 tgtagttgtg caccgccgag gcgctattgc cgttgatatt gctgacgctg gccagtgcgg
    21901 aggcgctgcc ctcgggtaat gacgccagat cggcggcggg tacggtggca gtccagctac
    21961 catcgctcgc tacgctggca gtgtaatttt tcccgccaaa ggtgacggtg acggtttgac
    22021 cgggttctac gttagtggtg gtgccggaaa gggttaaatc agcgcctttt tctgcggcgt
    22081 taatcacatc gtccgtggcg atggtgttga tggtgatcgc tgccggagtg agatccaccg
    22141 tcaccggatg cgtaatgctc accgagtttc cggcgctact ttcccctgaa acggcaatat
    22201 taaccgtccc cgcaggccag gcgctaacct gcgccgccgt gacgcccacg ctccagctac
    22261 catcggcctg caccgcagtg gtgtattcca cattattaat cgtaacggta agcggcgtgc
    22321 tttcagccag ccccgagctg ctgccggtga ccaccagcgc ctgcccgtgc tcgatgatat
    22381 tgaccacatc atcgcccgcc accgtatcga cccgcaggcc gggcagattg gcgtcgatgg
    22441 tgatatcccg cgtgccgctg ccggtgttgc cgttttgatt ggtgactgag gcattaaccg
    22501 ttaaatcgcc atttcccagc gcctgaatat ctgccgctgg aacgtccacg ctccaggtga
    22561 gattgctgcc caccgtcgcc gtataagtat taccacccaa cgtaatagtt accgtgtcgc
    22621 catcttccgc gccagtcacc tgcccgctga tggtttgcgc cacgcccgct tcggcggcgt
    22681 taataatatc gtcggttgcc accggattaa tggtcacacc gggcagcgcg ctgtcgacca
    22741 gcacgttatg gctggcagtg gcgctgttgc cgatatcgct ggtcaccgcc gccgttaccg
    22801 tatagttggc ctggcctaat gctgtaaccg ccgatgccgg aacggtgacg ctccagttgc
    22861 cggaggcgtc ggtcgtggtg gtgtaatttt gcccgttcag cgtcacggtg atggcggtgt
    22921 taacaggctg atcgctggtg ccggtaatct ggagatcggc ccctttttcg ctggcgttaa
    22981 tcacatcatc gcccgcaatg ctgttaatgc cgataagggg cgcggcggta ttgaccgtga
    23041 cggtcaatga ctggctgccc gtgtttccgg ctttgtcggt taccgagacg ctgacaggat
    23101 aactgccgtc cgccagccca ctgaccaccg aggccggaac gcccagactc cagttgccgg
    23161 aaccatccac caccgtggtg taatccacat tattcagcgt cacggtgacg atatcgcccg
    23221 cctgagcgcc agtaaccgtg ccgctgatga tttgcgcctg agcatgttcc ggcgtgttga
    23281 tgacgttatc ggtcgccacc ggattaatgg ttaccaccgg cggcgtggca tccaccagcg
    23341 ccacgcgtga aacgctgctc ggattaccgg cgacatcgtt cacgctggcg gtgaccgtag
    23401 cgttgccgtc agccagtgcg cccacgttgg cggcgggcac agtcacgctc caggtgccgt
    23461 ctggctgcac gacgccctga tacgtctgcc catttaacgt aacggtcacc gtctgccccg
    23521 gctgagcgtc ggtggtgccg gtgatgggca gatcctgccc gctttcggcg gcgttaatga
    23581 tgttatcagc ggtcagtgga tcaatgctta aggccggggc ctggctatcg acggtaatgg
    23641 tgcgggaagt gttgccggta ttgccgctga gatccgtcac agaagcggta atcgccaccg
    23701 cgccgtcagg cagatttccc atcgcggcgg caggtaccgt cagactccag gagccatcgg
    23761 cctgcacggt agtggtaaaa ctctgtccag ccagggttac tgtgacggtt tgtcctgtct
    23821 cagcctgttg agtgctaccg gtaagggtca gtggcgcacc tttttctgcg ccactgataa
    23881 tgtcatcgcc ggagacggtg ttaatggtga tcaacggaga aacaccgacc agcgtgattg
    23941 ctcgagacgc cgcgccagta tttcctgcag catctgtcac ggtgacgaaa atcgtattgg
    24001 ctccgcgatc cagcgtgcgg gttaccgctg ggtccagcgc cacattccag ctgccatctg
    24061 ccagcacgat gcccgtcagg acatgagtgc ccaatttcac cgttaccgta tcgcctggag
    24121 aagcaccgct gacttgtccg gagatgatct gcgcgacggc ctgctcggca ttattaagaa
    24181 tatcgtcgcc cgccacggta ttaacactga ccacgggtgc tgaggtgtct accgtgaaat
    24241 tcgcgctgtg gctggtggtg ttccctgccc gatcgctgac actggcattg acagcgtaat
    24301 taccctccgc cagtgcctgg acttccgtgg caggcagcgt cacttgccag gttccgtcgc
    24361 taccgacggt tgctgtatgg cttttcccgt tgacggtcag tgttacggtt tgccccgctt
    24421 ctgcattcga cgtgccgctc agtaccagcg cgacgttatg ttccgcagca ctgataatgt
    24481 tgtcctgagc gatggtgttg atggcaagcg ttggcggttg ggtatcgacc gttagtagct
    24541 gcgcgccggt gaccgtattg cctgtggcgt ctttcccact gaccagcacc gcctgattcc
    24601 cttcgcctaa cgcctgtgca tcggcggtcg gcacctgcac gctccaggca ccgttagcgc
    24661 ctacgctggt cgtgtaggtt ttgccgttta gcgtaacggt gagttctgtt ccctgcgcca
    24721 gatggctact ggtgccagaa agcgtaaacc cggcggcctg ttcgctggcg ttaataatgt
    24781 tatcgcctgc ggtggtggca acctgcacgc tggcctgact attgtcgatg gttaacgtta
    24841 tcgaaccggc gtcagtgatg gtactgccga caatttgcac cactgaccag gtatgttcac
    24901 ctgcactttg cgtgggtaac gagacactcc agtgaccgtt gttgtctacc tgagtactgg
    24961 caatagtatt gccgttactg tctttaatct gaatagtcgc ccccgcctgc ccgctgccgc
    25021 taaaggttgg cgtggtgtca tcggtgatgt cattggtggc tagaatgccg cgctgatcgc
    25081 cctgattatc ggtaactaaa aatgatggtg tggcgttgcc aggctcagca ggtggcgtag
    25141 ggttattaat cacttctgtt ttcgagtcgc catcgccacc gcttgccagc agtgcaccaa
    25201 gcgcgccacc acctaccgcc gcccccgcca gccaacccca cgggaaggcg ctggtctgag
    25261 caacggtatc aagaaatggc gcaatgcttt caatcgcagt ggtctgggca gtcagttcta
    25321 cgggggctaa tccacccgca gcagtatcgg caaaggtgat atgggttagc tgttgaccat
    25381 cggcaaacac cagttccgat tgttcagctg tattcgccgc ttgcaggaaa taaccgttgc
    25441 agcggatcac cgtgccgtcc cgcatataga tcagcaggtc atttccttca cggacgtaac
    25501 gcgcaacggc agaggcctga gcatgaacct gcaccacgga aggctcataa ataacaaccg
    25561 tgacgtcgcc aggattaatc agcgtttgtt tgacggatgt tttacgcgaa ataacatcaa
    25621 taatcaggct cataatggca ttcctgataa atggcgcaca tccttagcga gtccatttat
    25681 ctaaataaga caaaggtaat cacttaacaa cggcccacta ttaataatgg caggattatt
    25741 tcaagtcggg taagtcgttg tataagccct gtttaattcc cgctaatgga ataaggttat
    25801 tcaccgcagc ggcataattt acggcggcga cccagccatc ataattggca tttatttcag
    25861 ccgattgcgc ctgaaagaca tcttgttcga cggttaatag atcattcaga ctgcgtttac
    25921 ccaatttata ttcattttgg tacacatctc gcgttttgtg cgcactttcg gattgcgcta
    25981 aaccggcttc ttcacgaccc cgtgcgccgg tccagttcgc atatgccaca gacgctcgtt
    26041 gcagcacatc cagttttgcc tgttcgacct gcgaggcaga gattttttgt tgcccttcgg
    26101 cctgttgcac ctgggcagaa accgcgccgc cctgatacag cggcgcgtta acgttcagtt
    26161 gtagctgatc atcccaatac gagcggtcgc tggtctggta gcgcgtttta cccccctgaa
    26221 tactgagcgt tggccagtat tgcgctttcg ttttttccac gccgtactgt gctgactggc
    26281 gtaagttttc tgccgccagc accagcggga tggactggta atcaatattc ttcagcgata
    26341 ccggctgctc ggctaattca gcaggtggcg cggctatcgc ctccggctgt acgccagtga
    26401 gcaccgccag ttgcgctttg gcgctcgcca tctgcgcctg atattgctcc agcgttgagc
    26461 gcattccggc aatacgcgtc tgggcctgta attcatccga cgatgagttc aggcctgcgt
    26521 tagcgcgcaa tgccgccatg ttgtagacgt tttccagcga gtgaatattg cgttgagccg
    26581 catcgcacaa agcctgataa cgactaactt ccatgtaggc agtcgccgtt ttctctgcaa
    26641 catcggttaa ggttgccatc aatttaaagc ggtagctgtc acgggcggca gtttgcagat
    26701 tgatatcgtt gttagtttta ccaaagtcat acaccagttg tgtcagggtt atgccatacg
    26761 aaacgttgtt atcaagcctg ccgctggagt ccgtttgtcg ggacggcccc gcgttgccgg
    26821 ttaatccaac gtgcggatac caggcacttt tggcttcatc aatttgcgct tcgccaatac
    26881 ctatctgcgc cgcctgttga gtgacaccgg gatttcgcgc aaaggcgcgt aatatacttt
    26941 cctgtaaaga taattgggca actggcgtta ccgctggggc attcgcccat tgtggcagcg
    27001 gtgcagcaat tgcagcttct cccatcacca taaaacttat aaaaaaaata agtccattat
    27061 ttttacggtt tttaaactgg aatcgcgcca tagataagtc acttccttcc aggtcattat
    27121 tttaact
//
LOCUS       DABSYT020000060        26399 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.060, whole genome shotgun sequence.
ACCESSION   DABSYT020000060 DABSYT020000000
VERSION     DABSYT020000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.060"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            155..664
                     /locus_tag="ID725_RS14990"
                     /old_locus_tag="pgaptmp_002998"
     CDS             155..664
                     /locus_tag="ID725_RS14990"
                     /old_locus_tag="pgaptmp_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGV7851541.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            661..2079
                     /gene="phrB"
                     /locus_tag="ID725_RS14995"
                     /old_locus_tag="pgaptmp_002999"
     CDS             661..2079
                     /gene="phrB"
                     /locus_tag="ID725_RS14995"
                     /old_locus_tag="pgaptmp_002999"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGV7851542.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2229..3710)
                     /gene="dtpD"
                     /locus_tag="ID725_RS15000"
                     /old_locus_tag="pgaptmp_003000"
     CDS             complement(2229..3710)
                     /gene="dtpD"
                     /locus_tag="ID725_RS15000"
                     /old_locus_tag="pgaptmp_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGV7851543.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3981..4724
                     /gene="ybgI"
                     /locus_tag="ID725_RS15005"
                     /old_locus_tag="pgaptmp_003001"
     CDS             3981..4724
                     /gene="ybgI"
                     /locus_tag="ID725_RS15005"
                     /old_locus_tag="pgaptmp_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGV7851544.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4747..5403
                     /gene="pxpB"
                     /locus_tag="ID725_RS15010"
                     /old_locus_tag="pgaptmp_003002"
     CDS             4747..5403
                     /gene="pxpB"
                     /locus_tag="ID725_RS15010"
                     /old_locus_tag="pgaptmp_003002"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGV7851545.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5397..6329
                     /gene="pxpC"
                     /locus_tag="ID725_RS15015"
                     /old_locus_tag="pgaptmp_003003"
     CDS             5397..6329
                     /gene="pxpC"
                     /locus_tag="ID725_RS15015"
                     /old_locus_tag="pgaptmp_003003"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGV7851546.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKSRQDQQRYFEQLAWRLHNEN"
     gene            6319..7053
                     /gene="pxpA"
                     /locus_tag="ID725_RS15020"
                     /old_locus_tag="pgaptmp_003004"
     CDS             6319..7053
                     /gene="pxpA"
                     /locus_tag="ID725_RS15020"
                     /old_locus_tag="pgaptmp_003004"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGV7851547.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7089..7879
                     /gene="nei"
                     /locus_tag="ID725_RS15025"
                     /old_locus_tag="pgaptmp_003005"
                     /pseudo
     CDS             7089..7879
                     /gene="nei"
                     /locus_tag="ID725_RS15025"
                     /old_locus_tag="pgaptmp_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
     gene            complement(7876..8922)
                     /locus_tag="ID725_RS15030"
                     /old_locus_tag="pgaptmp_003006"
     CDS             complement(7876..8922)
                     /locus_tag="ID725_RS15030"
                     /old_locus_tag="pgaptmp_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGV7851548.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9071..10132)
                     /locus_tag="ID725_RS15035"
                     /old_locus_tag="pgaptmp_003007"
     CDS             complement(9071..10132)
                     /locus_tag="ID725_RS15035"
                     /old_locus_tag="pgaptmp_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7851549.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AAPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10129..10860)
                     /locus_tag="ID725_RS15040"
                     /old_locus_tag="pgaptmp_003008"
     CDS             complement(10129..10860)
                     /locus_tag="ID725_RS15040"
                     /old_locus_tag="pgaptmp_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGV7851550.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10875..13334)
                     /locus_tag="ID725_RS15045"
                     /old_locus_tag="pgaptmp_003009"
     CDS             complement(10875..13334)
                     /locus_tag="ID725_RS15045"
                     /old_locus_tag="pgaptmp_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGV7851551.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWMGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13384..13950)
                     /locus_tag="ID725_RS15050"
                     /old_locus_tag="pgaptmp_003010"
     CDS             complement(13384..13950)
                     /locus_tag="ID725_RS15050"
                     /old_locus_tag="pgaptmp_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7851552.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            complement(14337..15620)
                     /gene="gltA"
                     /locus_tag="ID725_RS15055"
                     /old_locus_tag="pgaptmp_003011"
     CDS             complement(14337..15620)
                     /gene="gltA"
                     /locus_tag="ID725_RS15055"
                     /old_locus_tag="pgaptmp_003011"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGV7851553.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(15826..15933)
                     /locus_tag="ID725_RS15060"
                     /old_locus_tag="pgaptmp_003012"
     CDS             complement(15826..15933)
                     /locus_tag="ID725_RS15060"
                     /old_locus_tag="pgaptmp_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851554.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16314..16718
                     /gene="sdhC"
                     /locus_tag="ID725_RS15065"
                     /old_locus_tag="pgaptmp_003013"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             16314..16718
                     /gene="sdhC"
                     /locus_tag="ID725_RS15065"
                     /old_locus_tag="pgaptmp_003013"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGV7851555.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16712..17059
                     /gene="sdhD"
                     /locus_tag="ID725_RS15070"
                     /old_locus_tag="pgaptmp_003014"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             16712..17059
                     /gene="sdhD"
                     /locus_tag="ID725_RS15070"
                     /old_locus_tag="pgaptmp_003014"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGV7851556.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            17059..18825
                     /gene="sdhA"
                     /locus_tag="ID725_RS15075"
                     /old_locus_tag="pgaptmp_003015"
     CDS             17059..18825
                     /gene="sdhA"
                     /locus_tag="ID725_RS15075"
                     /old_locus_tag="pgaptmp_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGV7851557.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            18841..19557
                     /gene="sdhB"
                     /locus_tag="ID725_RS15080"
                     /old_locus_tag="pgaptmp_003016"
     CDS             18841..19557
                     /gene="sdhB"
                     /locus_tag="ID725_RS15080"
                     /old_locus_tag="pgaptmp_003016"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGV7851558.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            19858..22659
                     /gene="sucA"
                     /locus_tag="ID725_RS15085"
                     /old_locus_tag="pgaptmp_003017"
     CDS             19858..22659
                     /gene="sucA"
                     /locus_tag="ID725_RS15085"
                     /old_locus_tag="pgaptmp_003017"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGV7851559.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            22674..23891
                     /gene="odhB"
                     /locus_tag="ID725_RS15090"
                     /old_locus_tag="pgaptmp_003018"
     CDS             22674..23891
                     /gene="odhB"
                     /locus_tag="ID725_RS15090"
                     /old_locus_tag="pgaptmp_003018"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGV7851560.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            23985..25151
                     /gene="sucC"
                     /locus_tag="ID725_RS15095"
                     /old_locus_tag="pgaptmp_003019"
     CDS             23985..25151
                     /gene="sucC"
                     /locus_tag="ID725_RS15095"
                     /old_locus_tag="pgaptmp_003019"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGV7851561.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            25151..26020
                     /gene="sucD"
                     /locus_tag="ID725_RS15100"
                     /old_locus_tag="pgaptmp_003020"
     CDS             25151..26020
                     /gene="sucD"
                     /locus_tag="ID725_RS15100"
                     /old_locus_tag="pgaptmp_003020"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGV7851562.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         6387 a         6596 c         7196 g         6220 t
ORIGIN      
        1 tcgtaatctt gccctggtat tcgctgcttt gatgatgatg taaaatgatt gtttttgaac
       61 tggatataac agcttggtag ggaagaaagc ataaccccac aaaattcatt taccatcgtc
      121 tacacttact cctgtaaacc gttcaggagc agtaatgaat cagcaacgat ttgatgacag
      181 cactctaatc cgtatcttcg cgcttcatga gttacatcga ctgaaagaac atggcttaac
      241 gcgcggggcg cttctcgatt atcacagccg ctataaactc gtatttctgg ctcattccca
      301 gccggaatac cgcaaacttg gcccgttcgt ggctgatatt caccagtggc aaaatctgga
      361 tgatttttac aaccagtacc gccaacgcgt aattgttttg ctttctcatc ccgccaaccc
      421 gcgcgatcac accaacgttt tgatgcacgt tcagggttat tttcgcccgc atattgagtc
      481 cacagaacgc cagcagctgg ctgcgcttat cgacagttat cgccgtggcg agcaaccgct
      541 tcttgcgccg ctgatgcgta tcaaacacta tatggcgctt tatccttacg cctggctttc
      601 agggcagcgt tatttcgaac tttggccgcg tgtgattaac ttgcgccatt caggagtttt
      661 atgactaccc atctggtctg gtttcgccag gatttacgtc tgcatgataa tctcgcgctg
      721 gctgccgcct gccgcaattc atctgcacgc ctgctggcgt tgtatatcgc tacaccacgc
      781 cagtgggcgg cgcataatat gtcgccgcgt caggctgaac ttatcaatgc tcaactgaac
      841 gggctacaaa tcgcgctggc ggaaaaaggc attcctttat tgttccgtga agtggatgac
      901 tttgccgcca gcgtcgaaat agttaaacag gtgtgcgcgg aaaacagcgt tactcatctt
      961 ttttataact atcagtatga ggtgaatgag cgggcgcggg atgtgcaggt tgagagaact
     1021 ctgcgtaacg tggtgtgtga aggatttgat gacagcgtga tccttccgcc tggcgcggtg
     1081 atgaccggca atcatgagat gtacaaagtc tttacgcctt ttaagaatgc ctggctgaaa
     1141 cggctgcggg aagggatgcc ggagtgcgtc gctgcaccaa aagttcgtag tagcggatcg
     1201 ataaagcccg cgccatccat tacgctgaat tatcctcgtc agtctttcga tactgcgcat
     1261 ttcccggtgg aagaaaaagc ggcgattgcg caattacgcc agttttgcca gaacggtgcc
     1321 ggagaatatg agcaacaacg agattttccg gcagtggaag gcaccagtcg tttgtccgcc
     1381 agcctggcaa cgggcgggtt atcgcctcgc cagtgtttgc atcgcttgtt ggcggaacag
     1441 ccgcaggcgc tggacggtgg ggccggtagt gtctggctta atgagctgat ctggcgcgag
     1501 ttctaccgtc atctgatgac gtattacccc tcgttgtgta aacattgtcc gtttattgcc
     1561 tggacggatc gtgtgcagtg gcagagcaat cccgcacatt tacaggcctg gcaggaaggc
     1621 aaaacgggat acccgattgt cgatgctgcc atgcgtcagc ttaacagcac tggctggatg
     1681 cataacaggc tacggatgat tacagccagt tttctggtga aagatttgtt gatcgactgg
     1741 cgcgaaggcg agcgatattt catgtcgcag ctgattgatg gtgatttggc agccaataac
     1801 ggtggctggc agtgggcggc ttctacgggt actgatgcag cgccgtattt tcgtattttc
     1861 aacccgacaa cccagggcga gaaatttgac cgcgaaggcg agtttatccg ccagtggcta
     1921 ccggaactgc gcaatgtacc ggggaaatcg gtgcatgagc cgtggaagtg ggctgagaaa
     1981 gcaggtgtga agctggatta tccgcaaccg atagtcgagc acaaagaggc gagagtacaa
     2041 acgttggcag cgtatgaggc ggcgcggaag gggaaataac agacgcgtca ggcaatcgag
     2101 cccagatgcc ggatgcggcg tgaacgcctt atccagccta caaatttgtg caaattcaat
     2161 aaattgcagg aaatacgtag gcctgataag acgcgtcaag cgtcgcatca ggcatcggtg
     2221 ctggcctatt aagactccag cgccagcgcg cggtttctga atttcagcgc ctgatacagc
     2281 caaatcatca gtaccactcc tacacacgcc agtgcgcccc aggtaatttg atcaaatact
     2341 tcaatatatg cattgatgga gtagttgatc gccccggaag catcaaacga agcctgcgat
     2401 gtctgatcgg caatcacgcc cgccagatag ttcgcaatcg cgccggaaag cagcatataa
     2461 atgccggtta atacgccggt cacaccgggg atttcaatgc gcgtaatctg cgacatagca
     2521 accgggtcga taaacagttc cgcaaagccc atcaccgcca ggcctaatac catcagtggc
     2581 agagaagagt gaccatacat tgcggaccag cgggcgctta aggtcagaat gcagaatccg
     2641 gcgctcatca ggccaaggcc aagagcaaat tttccccaga tgcgcacggt acgattaccc
     2701 gcgacactct cttttaccac ccacgccagg aacacaccgc acagcataac tgcgaaggca
     2761 ttaatcgact ggaacatcgc ggtcggaacg gtataaccaa acatatcgcg attaacgaag
     2821 cggtcgatat aaaggctaat cgagctaccg ccctgttgtg cgaaggccca gaacaacata
     2881 ctgaagaagg tgagcgtcac aatcagcctc agctccttcc gctgtttctg gttttctgct
     2941 ttgcgataaa tttttgccag tacacccagg ccaatgatag tcgcgacaat caaggcgtat
     3001 accgaccatt ctttccagaa cagtacggta atcagcgcag gcgttgccac cagcagaacc
     3061 agcagccatc cccagttcgg cagcagaaag tttgtcgcac gcagtacttt tttgttaacg
     3121 ccgcgggtat gtgtgaaatg acgattgcca cataagaaaa taaccagacc cgcgatcata
     3181 cccaccgccg ccaggccaaa gcccatcgcc caactgtact cttcctgggc gtagccacag
     3241 gcaataggtg cgataataga ccccacgtta cccgctgcat acatcagcga gaaaccgcca
     3301 tcacgacgcg gatcggttgg ctcatacagc tcgccgagca ggcaactgac attggattta
     3361 aacagaccat agccacaaac gattatcgcc agggacagat agaggaatga cggatggatc
     3421 tcacttgcac ccaacaccac atgaccgatc gccatcaata acgcccccag catcaccgcc
     3481 atgcgattgc cgagaacttt atccgccaaa aagccaccga ggattggcgt gacatacacc
     3541 agcgaacagt aggcgctaaa taactcgtag gcgtgagtat cgttgtattt tagttgattg
     3601 gtgagataga gaatcagcag ggcacgcatg ccgtaaaagc taaaatattc ccagatttgc
     3661 agcgcaacga catagtaaat agcgcgcggt tgtgatgcgt gtttattcat aataattcca
     3721 caattgacgg caacgtcacg taatagttgc gcggcgtgct tgtgtttcct taaagttaaa
     3781 actttgatac agatctgatt atttatgcaa tatgctgtct gattgcataa atatacatta
     3841 gctgaagcgt gatgatataa acaatttgcg tcttttggcg tcgggtttgt ttcttaatat
     3901 gtcaaaagat tggctatcga cgaacaggcg aggtaacgct atgttaacgg tgtagctatc
     3961 agaatacgga aggcgcgaag atgaaaaaca ccgaactgga acaactgatt aacgaaaaac
     4021 tgaacagcgc ggcgattagt gattacgcgc cgaatggttt gcaggtggaa ggcaaagaga
     4081 cggtgcaaaa aattgttacc ggtgtcactg ccagccaggc tttgctcgat gaggcagtgc
     4141 gtctgggcgc ggatgcggtc attgtgcatc atggctactt ctggaaaggc gaatctccgg
     4201 tcattcgcgg catgaagcgt aaccgtttaa aaacgttgct ggcgaatgat atcaacctgt
     4261 atggctggca tttgccgctt gacgcacatc ctgagctggg caataacgca cagctggcgg
     4321 cgttactggg gatcacggtc atgggcgaaa ttgagccgtt ggtgccgtgg ggcgaactga
     4381 ccatgcctgt gccgggactg gagctggctt cctggattga agcgcgtctg ggacgtaagc
     4441 cgttatggtg tggcgatacc ggacctgagg tggtccagcg cgtcgcctgg tgcacgggcg
     4501 gcgggcaaag ttttatcgat agcgccgcgc gttttggcgt ggatgctttt attactggcg
     4561 aagtttctga acagaccatt cattcagccc gcgagcaggg attgcatttt tatgctgcag
     4621 gtcaccatgc cactgaacgt ggtggtattc gcgcattgag cgagtggctg aatgaaaata
     4681 ccgatcttga tgtgaccttt attgatattc ctaatcctgc ataacgaata atcagaggga
     4741 tcgaaagtgc aacgagcgcg ttgttatctg ataggtgaaa cggcggtagt gctggaactg
     4801 gaaccgccgg tgacgctggc tagccagaaa cggatctggc gactggcgca gcgtctggtg
     4861 gatatgccga atgtggttga agccattccc ggcatgaaca atatcacggt gattttgcgt
     4921 aatcctgagt cgctggcgct ggatgccata gagcgtttgc aacgctggtg ggaggagagc
     4981 gaggccctgg aacctgagtc tcgctttatt gaaattccgg tggtttacgg tggtgcaggc
     5041 ggaccggatt tggcggtggt cgcggcgcat tgcgggttga gcgaaaaaca ggttgttgaa
     5101 ttgcactcct ccgtggaata cgtggtctgg tttttaggtt ttcaaccggg cttcccgtat
     5161 ctcgggagtt tgccggaaca actacacacg ccacggcgcg ctgaaccgcg cttgctcgtt
     5221 ccggcaggtt ctgtcgggat cggcgggccg cagactggtg tttatccgct ggcaacgccg
     5281 ggtggctggc agttgattgg tcatacctca ctcagcctgt ttgatccggc gcgtgacgaa
     5341 cccatcttat tacgtccggg agacagcgtg cgctttgtac cgcaaaagga gggagtatgc
     5401 tgaagattat tcgtgcgggc atgtatacca ctgtgcaaga tggcggtcgt cacggttttc
     5461 gccagtcggg tatcagccac tgcggcgcac tggatatgcc tgcgttacgc attgctaacc
     5521 tgctggtggg taatgatgcc aatgcccccg cgctggagat cacgctcggt cagttaacgg
     5581 ttgagttcga aactgatggg tggtttgctc tgacgggtgc cggttgcgaa gcgcggctgg
     5641 atgataatgc cgtctggacc ggctggcgat tgccgatgaa agcaggccag cgtttaacgc
     5701 ttaaacgccc gcagcacggg atgcgcagtt atctggcggt cgcgggtggt attgatgttc
     5761 cgccggtaat ggggtcatgc agcaccgatc tcaaagtggg gattggcggg ctggaaggcc
     5821 gtttactgaa ggatggtgac cgactcccga ttggcaaagc gaagcgtgat tttatggaag
     5881 cgcagggcgt taaacagctg ctgtggggca accgcattcg cgccttgccg gggccggaat
     5941 atcatgagtt cgatcgcgcc tcgcaggatg cattctggcg ttcgccctgg cagcttagct
     6001 cgcaaagtaa ccgcatgggc tatcgcttac aggggcaaat tttaaaacgc accaccgatc
     6061 gcgaactgtt atctcacggt ttgttaccgg gcgtggtgca ggtgccgcat aacgggcagc
     6121 ccattgtgtt gatgaacgac gcacagacca ccggtggtta cccgcgtatt gcctgtatca
     6181 ttgaggctga tatgtaccat ctggcgcaaa ttccgctcgg tcagccgatt cattttgtcc
     6241 agtgttcact ggaagaggca ctgaaatcgc ggcaagatca gcaacgttat ttcgaacaat
     6301 tagcgtggcg gctgcacaat gaaaattgac ctgaacgccg atctgggcga aggctgcacc
     6361 agcgacgcag agctattaac gctggtttcc tctgccaata ttgcctgtgg atttcatgca
     6421 ggcgatgcgc aaaccatgca ggcttgcgtg cgtgaagcaa taaaaaatgg tgtcgcgatt
     6481 ggcgctcatc cgagttttcc cgacaggaaa aattttggtc gcagcgctat gcagctgccg
     6541 ccagaaaccg tttacgccca gacgctgtat caaattggcg cgctggcaac gattacccgt
     6601 gcgcaaggtg gtgtaatgcg tcatgtcaaa ccgcacggca tgttatacaa ccaggcggcg
     6661 aaagaggcac aactggcaga cgccatcgcc agagcggtat acgcttgcga tccagcattg
     6721 gttctcgttg ggctggcagg aagcgagctg attcgtgcag gcaagcaata tggcctgaca
     6781 acgcgcgagg aagtgtttgc cgatcgcggt tatcaggctg acggctcgct ggtgccgcga
     6841 agccagtcag gcgcgctgat tgaagacgaa gaacaggcgc ttgcgcaaac gttggagatg
     6901 gtgcaacacg gcagagtcaa aagtatcacc ggcgaatggg caacggtcac ggcgcaaacg
     6961 gtctgcctgc atggcgacgg cgagcacgca ctggcgttcg cccgccgact acgctctgca
     7021 tttgccgaaa aggggattgt tgtcgcagca taaccccgat taataaagaa tgaaaaaagg
     7081 atatcaccat gcctgaaggc ccggagatcc gccgtgcagc ggataacctg gaggcggcga
     7141 tcaaaggcaa accactaact gatgtctggt ttgccttccc gcagttaaaa acttatcaat
     7201 cacaacttat cggtcaacac gttacccatg tggaaacgcg tggtaaggcg ttgttaactc
     7261 atttttccaa cgacttaacg ctctacagcc ataatcagct ttatggcgtc tggcgcgtgg
     7321 ttgataccgg cgaagagtcg cagaccacgc gagtattgcg ggtaaaactg caaacggctg
     7381 acaaaaccat tctgctttat agcgcctcgg atattgagat gttgaccccg gaacaactga
     7441 ccacgcatcc gtttttacaa cgcgttggtc ccgatgtgct ggatccgaat ctgacgccgg
     7501 aggtggtgaa agaacggtta ttgtcgccgc gctttcgtaa ccgtcagttt gctggattac
     7561 tgctcgatca ggcgtttctg gctgggcttg gcaattattt gcgtgtggag atcctctggc
     7621 aggttgggtt gactggaaat cataaagcga agacctcaat gcggcgcaac tggatgcact
     7681 cgcacacgcg ttactggata ttcctcggtt ttcttacgct acgcgggggc aggtggatga
     7741 gaataagcat catggggcgc tgtttcgctt taaggttttt catcgtgatg gcgaactgtg
     7801 cgaacgctgt ggcggcatca ttgagaaaac cacgctgtca tctcgcccgt tttactggtg
     7861 tccgggctgc cagcactagg ccgtacgttt cggcgcatag gttgaaataa accgcgcaat
     7921 ggcaggccct gtcagcaaaa tactgaacag gcgcagggtt tgcatcgcca taatgagcgc
     7981 catatcggca ttgctccctg cggcgatgac cgccacggta tcaagcccgc cggggctggt
     8041 ggcgaggtag gcagtcataa aatcaatgtg cataaaccgg gtcagtcccc acgccatacc
     8101 cgcacaaata gccagcagag caaaaatcga cagcaggatt tgtggtagcg ggcgtagtgc
     8161 ccgcagtaag atttgtttat cgaaaccaag gccaatccgc cagccaattg ccatatacgc
     8221 catcgccagt agccattccg gtagttcgat ggtgatgagc tgaccagact ggagcactgc
     8281 gcccgccagc atcgggatga gcatcgtgcc ggaaggaaga cgcagcaggc atcccactgt
     8341 accggcaacg accgcgagca aaatagtaag caggagatta atgctcactg gcgggaacca
     8401 gacgatatgc tggttaaccg cttcagcgtt atcgcccagc atcattcgcg tgaccagaac
     8461 tgcggccccc gcgacaaaca gcactcgcag atactgcata aacgccacca ggcggatatc
     8521 tgcgccgtaa tcctgcgcca tcgcgaccat tgccgccgcc ccgccaggcg aggagcccca
     8581 ggcaccagta tttccgggca gtgagctata gcgcaccagt agccatccca cgatagcgct
     8641 ggaaagcaac gtcaccagta aaatagctaa cacaatcggc cagttaacct ccagcgtggt
     8701 caggatcgaa ccggtcaagt tttgcgcaat catgcagcca agaatggcct gcgcggcgag
     8761 aaaagcggag cgggggagtt gcagggtaac cccgcgcata ctgaagatga tcccggcgat
     8821 catcggtcca agtaagagtg ccgccgggag atgtaatccg aggaaaccaa tagaaaggag
     8881 aagtgatagc acacataaca ttccccactg caaaactggc atcctttgct ccctcattat
     8941 tttctctttg gtgtatcgat acagcataaa gaatgccggg catggaaaaa ataacggtaa
     9001 tcaataaaag ccggaaaatt ggcaattaac cgaagaagcc ctctccggct ggaaagggca
     9061 ataggctaaa tcagttgtag ttaattttga aaagtaccac tgtatcaaag ggaccaacgt
     9121 cgatgggcgt acccggcgtg gatgacaatt ctgccgtgta ggttttttca tacactaaca
     9181 ggtcgctggt aaaatcggca tactcactgt atttgttgaa agtatagggt gaggcatttt
     9241 catccaacaa tcgaagcttc aagccattgc caatgagcag tgatttatct tcgtcgataa
     9301 gggtttcatc ggtataaaac gaagagctga ctttaaaacc gtcggtacac tggtcgtttt
     9361 gtgatttggt tgtagttatt ttaaattgct gcgacatcgt atggcgacga atatccagca
     9421 cgttaaatcg gccaaaatca acgacctggg tttcaggtga gatagcaaag ttgacgctgc
     9481 aatccagcac gcggatattt tccagaccgg taatgtgata tttcagattc ttcgcggtag
     9541 ggtcttcgtt cacgctgcct gcgccatcaa actgtacgac gatgtaatcg ctgagtttgc
     9601 tctgataatc atggggcggc atggcgcgaa ctttaacgta aagccgcata cgcgccacaa
     9661 acgtccgcga tttgtgcata acggttggat cgccggagca aagtttattt tgccagccca
     9721 tcgcaatgat ctgctccggg gtgtaaatat caatattctt actgtcgatg cactgattcg
     9781 tgtcgatgcg gcttttccca aggcttgcgt catagtccac gccttcatag gttacgccca
     9841 attgataata gggatcctga ataccggggt aagggtttac ccaggcgtag acgtgctcgc
     9901 tttcaaaatt accgtttgtg ttgttgtcac agtagaccgg aatttttata tcgtcagatt
     9961 cccagatttt atcgccgggt ttggaattgg ccggagcggc gaagggcata atagcttccg
    10021 atttttctac agcgccgttt gcggtaccaa aatagcagtt cagcgccagc gccgctttta
    10081 gcggcaaaaa aatcagacag atggcgagca acacgccttt ggcgaatctc attttttatt
    10141 ctccaccggc tgcaggcggc attgccgctg gcaggagagc gtgttcatac gcaatgcgcc
    10201 gtagtcgtcc atgtagccaa gataaaactg cttgccgcta tagctgcccg tgctggtggt
    10261 gaccgaactg aacggtgcga ccatcgtgct tttaaagcca ggcagcacgc ctttctcatc
    10321 ccgcccaaga taggcaatag tcagatagta tccggtcggg gttttcaggg taagctggtt
    10381 attctgctgg ctaacctgta gctgttgctc aacatgatcg cccatttttt tgcgtaatgc
    10441 ggcgggtcgc cagaatagct taatgcgact ttggacggcg acttgcagga tcgcatggcc
    10501 ctcgctgcct tccgggacag gcggaatttc acgcaggtta tagtaaaaga gagattcgcg
    10561 atcggtgggt aatttcgcgg tggcggcttg tttcatgatc cgaacctgcg aagtcgcttt
    10621 tggctccagt cgctggatag gcggcagggc gacgagaaat tcatcgcttt tctctccctt
    10681 ttcattttct atccatgaat aggcgaggta ggggagttta tcgctgcggt tttccacgcg
    10741 caggctggtt gctttatctt tgctattaaa gatgatgcgc gtgcggtcag gctgcactgc
    10801 gccgtggctg cacatactgg caaccagaag aatcaaaggg aatcctttaa caaatgtcat
    10861 cgtcttttcc ttaattaatg acagggtaaa atcaggcgct tagtcacatc ttccagatgt
    10921 tccggcagat gaatggcaca cgttttctgg tcgccccagt gcacggtaaa ttgttgattt
    10981 tcatcgaccc cactcagcca tgcgtgaccg ttttcgccaa ccatcccgac gctgacaccg
    11041 ccttcagcct ggcgaacatc ggcaccgagc ggcgggaaat gcccgttagc atcgctgata
    11101 atgacgttga gatctttccc ggcgcgggac gccagcgatt taaagccgat ggcgccttcg
    11161 gtccaggttt ctttcaccac gttttcggaa acggtaacgc cgtcaggcaa gtcgttcata
    11221 ttgaccgcca ccgtcgtagg ctggtagctg gaaacaaacg gtaccacggc aataccaaag
    11281 cggttggtgt agtcaatatt gccctgaatc ggaatatcgc ccacgccgtc ggtgctgacc
    11341 atcaggcgtg gttcattggt ggaactgcgg cgatggaatg ccgcgccatg ttgagtcgcg
    11401 gtaaacgagc cgctccaact ggcactggcg gaagtgtaat cattggcggc ataggttccg
    11461 gtcagatccc attcccccgc cgaactgagg tgttggtaat tgccgctgac ctgcgcaccg
    11521 ttatccgggc gatcggattg tattccagcc gacattcccc aactgtttcg ttcatccagg
    11581 gtatcgttcc acgacatgcg gtgggtggta gtattactgt tgttctgcat gtcataaccc
    11641 agtcgaccac tttccccaat cggtaaggag atcgaaaagt aaatctggtt gtcgcgatct
    11701 ttatcttcat aatgggtggt gttgaacgag gtcgagacag aaatatcttt ccagtcgcca
    11761 atgtcgacgt taaaaccgac ggtaatgttg gccgtggtgg aggtatccgc attccaccag
    11821 ctctgatgca ggatgttggc gtagagatta agattcagca gagtaatagg ctgaccgaag
    11881 gagaggctga ttgtttgctt ttcatcctgc gcgtcggcgt cattgtattt gtgatcaatg
    11941 tagttagcgt agctgtgaaa atggcgatcg gagaagcgat aggctgccaa tgagatggtg
    12001 ctgttggtcg catcaacctg tttgctgtag ttaaagcggt agctgtaacc ctgctcatcc
    12061 tgttgggtat caaaatggct gtcggcccag gtcacgtcaa acgacagcgc ccccatccag
    12121 agcatatttt gcccaatacc cagcgcgccg gaacgatagt catcgcctgc gagaagcatg
    12181 ccgccatata gtgaagtgtt ggagagcatt ccccaggaga cttcatggct aatgaaggtt
    12241 tcatcctcgg tgtggtgcga catagaggaa cgcgggcgac cggcagccag tttatagcgc
    12301 acctggccct gacgcgtcag gaacggggta gaggccgctg aaacctggaa gttattcacc
    12361 cgtccatctt cttcgctcac tttgacatcc agtgtacctt gcactgactg attcaggtca
    12421 tcaataataa atgggcctgg tggaactttt ttctggtaaa tcacgcgacc agaatggctt
    12481 atcgtgaccg tggcgttagt ctgtgcgatg ccgctgattt gcggcgcgta gccgcgtaac
    12541 tcccagggga gcattcgatc gtcgctggct aacgccgcac cggtatagga gaaaccatca
    12601 aaaatgttag agctaaaatc ggtttcgccc agggttaatc gggaacctat ttgcggtaac
    12661 gggcggaaca ggtaggtccg tgaaatcgcc ccagattgtt cacggttgtc gccgctatcg
    12721 gactggctaa gctgataatc gctgcgcaga cgccatgccc cggcgttgag gcccgtggtg
    12781 ccataggcgt tcaggttgtt gctgctgctg ccgctttgtg ggcgataagt gctggcaaac
    12841 aggttgtaat ccatcaggaa gccaggaata ccgttattcc acgttgaggg tggcgtccag
    12901 ttttctgaat gccacgccag ccatgcctgc gggattgtaa tattgagctg ttgatttgcc
    12961 tgatcgaaag taaagatgat ttcagggaag acactaaaat cgacacattc ttttattgcc
    13021 ggtaattttt tacgaaagtc agatttaagt ccgaagagtt cgatcagcga ttcattgata
    13081 caaggaataa ttttatcgcc agatttttgc cagcgaattt gttgcccatt actgatttta
    13141 ttattattaa ccgtaacact aacaaagtaa tcgccgggag ctacgacgcc tttttgattg
    13201 agcaacgaaa tatcaacgct atcccgaatc gacttatcga gaacgttaag gttaaattca
    13261 gctgtcagcg caggaggggt taccattgcc agacaggaaa gaacagagag tcgataaata
    13321 ttcacggcat ccatacctga acaatattcg caaaaggcgg ctaatcagcc gccttaaaaa
    13381 ggattattgg taatccagaa cgtaagttgc gttagcagta acggcccccg cggtaaccga
    13441 aactgcgtta tccatctgtt ccatccaggc aaagaagttt aacgtttgtt ctgttgaact
    13501 tgccaataag tcaattgttg gcgcttctgt tcccaatgta acaaatgatt catctttgtt
    13561 catgagacgt acgccaacgc cggtagcctc gccagcactg atgttactaa ggagcggggt
    13621 tgcacctgtc gttttagccg tgctgtcgaa ggtcacgcct acctttgaga ccacgccacc
    13681 cttgccatca tcccagcctg caatatcaca gttgattaag cgaatattga caggtttttt
    13741 ctcgctatga tgttctcgat ttatgtggga tgtggttact tcacccaggt caatgttcat
    13801 atcaaggtca tcagggtgaa tttcacatgg tgcttcaata acttcacctt taaagtggat
    13861 ttcaccagat ccttcatctg cagcaaaaac aggtgaagcg aatagtagag atatggccag
    13921 ggcggccagt gttttttgtc ctttaaacat acatgaatcc ttcatgggct atgataaagt
    13981 atactaatta ttccgacaga atagtggttt tttgttcatg taataatatt aatgttgcta
    14041 cattatgttt gagagtttct caaagctctc gaatcataaa taaagtgaat taatcctatt
    14101 acgagtttat ttaattgtgg gtgctaccca atgtattcta catcgattgg cgggataacg
    14161 gtggtggtga atgaaaaatt aggattttat ttagttatcg gatatgtatt cttgtaacgt
    14221 gtaattatgt atttgtgtac gattacgttt ttttgtcgat agacataact aacgaacaaa
    14281 aaaaagcccg ccagatattc aggcgggcta aacgatatat ggttcaaatc agataattaa
    14341 tgtttaacat ctgatttaaa gtctcgtttt tcatagccgg tgtacaactg gcgcggacgg
    14401 gcgatcttca tgccgtcact gtgcatttcg ctccagtgag cgatccagcc aacggtacgg
    14461 gccatagcga agatgacggt gaacatggaa gacggaatac ccatcgcttt caggatgata
    14521 ccagagtaga aatcgacgtt cgggtacagt ttcttctcga taaagtacgg gtcgttcagc
    14581 gcgatgtttt ccagctccat agccacttcc agcaggtcat ctttggtgcc cagctctttc
    14641 agaacttcat ggcaggtttc acgcattacg gtggcgcgcg ggtcgtaatt tttgtacacg
    14701 cggtgaccga agcccatcag gcggaaagaa tcatttttat ctttcgcacg acgaacaaat
    14761 tccggaatgt gtttaacgga gctaatttct tccagcattt tcagcgccgc ttcgttagca
    14821 ccaccgtgtg caggccccca cagtgaagca atacctgctg cgatacaggc aaacgggttc
    14881 gcacccgaag agccagcggt acgcacggtg gaggtagagg cgttctgttc atggtcagcg
    14941 tgcaggatca gaatacggtc catagcacgt tccagaatcg gattaacttc atacggttcg
    15001 cacggcgtgg agaacatcat attcaggaag ttaccggcat aggagagatc gttgcgcgga
    15061 taaacaaatg gctgaccaat ggaatacttg taacacatcg ctgccatggt cggcattttc
    15121 gacagcaggc ggaacgcggc aatttcacga tgacgaggat tgttaacatc cagcgagtcg
    15181 tgatagaacg ccgccagcgc gccggtaata ccacacatga ctgccattgg atgtgagtcg
    15241 cgacggaaag cgtggaacag acgggtaatc tgctcgtgga tcatggtatg acgggtcacc
    15301 gtagttttaa attcgtcata ctgttcctga gtcggttttt caccattcag caggatgtaa
    15361 caaacttcca ggtagttaga atcggtcgcc agctgatcga tcgggaaacc gcggtgcagc
    15421 aaaatacctt catcaccatc aataaaagta attttagatt cgcaggatgc ggttgaagtg
    15481 aagccagggt caaaggtgaa cacacctttt gaaccgagag tacggatatc aataacatct
    15541 tgacccagcg tgcctttcag cacatccagt tcaacagctg tgtccccgtt gagggtgagt
    15601 tttgcttttg tatcagccat ttaaggtctc cttagcgcct tattgcgtaa gactgccgga
    15661 acttaaattt gccttcgcac atcaacctgg ctttacccgt ttgttatttg gctcgccgct
    15721 ctgtgaaaga ggggaaaacc tgggtacaga gctctgggcg cttgcaggta aaggatccat
    15781 tgatgacgaa taaatggcga atcaagtact tagcaatccg aattattaaa cttgtctacc
    15841 actaataact gtcccgaatg aattggtcaa tacttccaca ctgttacata agttaatctt
    15901 aggtgaaata ccgacttcat aacttttacg cattatatgc ttttcctggt aatgtttgta
    15961 acaactttgt tgaatgattg tcaaattaga tgattaaaaa ttaaataaat gttgttatcg
    16021 tgacctggat cactgttcag gataaaaccc gacaaactat atgtaggtta attgtaatga
    16081 ttttgtgaac ggcctatact gccgccaggt ctccggaaca ccctgcaatc ccgagccacc
    16141 cagcgttgta acgtgtcgtt ttcgcatctg gaagcagtgt tttgcatgac gcgcagttat
    16201 agaaaggacg ctgtctgacc cgcaagcaga ccggaggaag gaaatcccga cgtctccagg
    16261 taacagaaag ttaacctctg tgcccgtagt ccccagggaa taataagaac agcatgtggg
    16321 cgttattcat gataagaaat gtgaaaaaac aaagacctgt taatctggac ctacagacca
    16381 tccggttccc catcacggcg atagcgtcca ttctccatcg cgtttccggt gtgatcacct
    16441 ttgttgcagt gggtatcctg ctgtggcttc tgggtaccag cctctcttcc cctgaaggtt
    16501 tcgagcaagc ttccgcgatt atgggcagct tcttcgtcaa atttatcatg tggggcatcc
    16561 ttaccgctct ggcgtatcac gtcgtcgtag gtattcgcca catgatgatg gattttggct
    16621 atctggaaga aacattcgaa gcgggtaaac gctccgccaa aatctccttt gttattactg
    16681 tcgtgctttc acttctcgca ggagtcctcg tatggtaagc aacgcctccg cattaggacg
    16741 caatggcgta catgatttca tcctcgttcg cgctaccgct atcgtcctga cgctctacat
    16801 catttatatg gtcggttttt tcgccaccag tggcgagctg acatatgaag tctggatcgg
    16861 tttctgcgcc tctgcgttca ccaaagtgtt caccctgctg gcgctgtttt ctatcttgat
    16921 ccatgcctgg atcggcatgt ggcaggtgtt gaccgactac gttaaaccgc tggctttgcg
    16981 cctgatgctg caactggtga ttgtcgttgc actggtggtt tacgtgattt atggattcgt
    17041 tgtggtgtgg ggtgtgtgat gaaattgcca gtcagagaat ttgatgcagt tgtgattggt
    17101 gccggcggcg caggtatgcg cgcggcgctg caaatttccc agagcggcca gacctgtgcg
    17161 ctgctctcta aagtcttccc gacccgttcc cataccgttt ctgcgcaagg cggcattacc
    17221 gttgcgctgg gtaataccca tgaagataac tgggaatggc atatgtacga caccgtgaaa
    17281 gggtcggact atatcggtga ccaggacgcg attgaatata tgtgtaaaac cgggccggaa
    17341 gcgattctgg aactcgaaca catgggcctg ccgttctcgc gtctcgatga tggtcgtatc
    17401 tatcaacgtc cgtttggcgg tcagtcgaaa aacttcggcg gcgagcaggc ggcacgcact
    17461 gcggcagcgg ctgaccgtac cggtcacgca ctgttgcaca cgctttatca gcagaacctg
    17521 aaaaaccaca ccaccatttt ctccgagtgg tatgcgctgg atctggtgaa aaaccaggat
    17581 ggcgcggtgg tgggttgtac cgcactgtgc atcgaaaccg gtgaagtggt ttattttaaa
    17641 gcccgcgcta ccgtgctggc gactggcgga gcagggcgta tttatcagtc taccactaac
    17701 gcccacatta acaccggcga cggcgtcggc atggctatcc gcgcaggcgt accggtgcag
    17761 gatatggaaa tgtggcaatt ccacccgacc ggcattgccg gtgcgggcgt actggtcacc
    17821 gaaggttgcc gtggtgaagg cggttatctg ctgaacaaac atggcgaacg ctttatggag
    17881 cgttatgcgc cgaacgccaa agacctggcg ggccgtgatg tggtggcgcg ttcaatcatg
    17941 attgaaatcc gtgaaggtcg cggctgtgat ggtccgtggg ggccacacgc gaaactgaaa
    18001 ctcgatcacc tgggtaaaga agttctcgaa tcccgtctgc cgggtatcct ggagctttcc
    18061 cgtaccttcg ctcacgttga tccggtgaaa gagccgattc cggttatccc aacctgtcac
    18121 tacatgatgg gcggtattcc gaccaaagtt accggtcagg cgctgactgt gaatgagaaa
    18181 ggcgaagatg tggttgttcc ggggctgttc gcggtaggtg aaattgcctg cgtatcggta
    18241 cacggtgcta accgtctggg cggcaactcg ctgctggacc tggtggtctt tggtcgcgca
    18301 gcgggtctgc atctgcaaga gtctatcgcc gagcagggcg cattgcgcga tgccagcgag
    18361 tctgatgttg aagcatcttt ggatcgtctg aaccgctgga acaataatcg taacggtgaa
    18421 gatccggtgg cgattcgtaa agcgctgcaa gaatgtatgc agcataactt ctcggtcttc
    18481 cgtgaaggtg atgcgatggc taaagggctt gagcagttga aagtgatccg cgagcgtctg
    18541 aaaaatgccc gtctggatga cacttccagc gagttcaaca cccagcgcgt tgagtgcctg
    18601 gaactggata acctgatgga aacggcgtat gcaacggctg tttctgccaa cttccgtacc
    18661 gaaagccgtg gcgcacatag ccgcttcgac ttcccggatc gtgatgatga aaactggctg
    18721 tgccactccc tgtatctgcc agagtcggaa tccatgacgc gccgaagcgt caacatggaa
    18781 ccgaaactgc gcccggcatt cccgccgaag attcgtactt actaatgcgg agacaggaaa
    18841 atgagactcg agttttcaat ttatcgctat aacccggatg ttgatgatgc tccgcgtatg
    18901 caggattaca ccctggaagc ggaagaaggt cgcgacatga tgctgctgga tgcgcttatc
    18961 cagctgaaag agaaagatcc cagcctgtcg ttccgccgct cctgccgtga aggtgtgtgc
    19021 ggttccgacg gtctgaacat gaacggcaag aatggtctgg cctgtattac cccgatttcg
    19081 gcactcaacc agccgggcaa gaagattgtg attcgcccgc tgccaggttt accggtgatc
    19141 cgcgatttgg tggtagacat gggacaattc tatgcgcaat atgagaaaat taagccttac
    19201 ctgttgaata atggacaaaa tccgccagct cgcgagcatt tacagatgcc agagcagcgc
    19261 gaaaaactcg acgggctgta tgaatgtatt ctctgcgcat gttgttcaac ctcttgtccg
    19321 tctttctggt ggaatcccga taagtttatc ggcccggcag gcttgttagc ggcatatcgt
    19381 ttcctgatcg atagccgtga taccgagact gacagccgcc tcgacggttt gagcgatgca
    19441 ttcagtgtat tccgctgtca tagcatcatg aactgcgtca gtgtatgtcc gaaggggctg
    19501 aacccgacgc gcgccatcgg ccatatcaag tcgatgttgt tgcaacgtaa tgcgtaaacc
    19561 gtaggcctga taagacgcgc aagcgtcgca tcaggcaacc agtgccggat gcggcgtgaa
    19621 cgccttatcc ggcctacaag tcattacccg taggcctgat aagcgcagcg catcaggcgt
    19681 aacaaagaaa tgcaggaaat ctttaaaaac tgcccctgac actaagacag tttttaaagg
    19741 ttccttcgcg agccactacg tagacaagag ctcgcaagtg aaccccggca cgcacatcac
    19801 tgtgcgtggt agtatccacg gcgaagtaag cataaaaaag atgcttaagg gatcacgatg
    19861 cagaacagcg ctttgaaagc ctggttggac tcttcttacc tctctggcgc aaaccagagc
    19921 tggatagaac agctctatga agacttctta accgatcctg actcggttga cgctaactgg
    19981 cgttcgacgt tccagcagtt acctggtacg ggagtcaaac cggatcaatt ccactctcaa
    20041 acgcgtgaat atttccgccg cctggcgaaa gacgcttcac gttactcttc aacgatctcc
    20101 gaccctgaca ccaatgtgaa gcaggtaaaa gtcctgcagc tcattaacgc ataccgcttc
    20161 cgtggtcacc agcatgcgaa tctcgatccg ctgggactgt ggcagcaaga taaagtggcc
    20221 gatctggatc cgtctttcca cgatctgacc gaagcagact tccaggagac cttcaacgtc
    20281 ggttcatttg ccagcggcaa agaaaccatg aaactcggcg agctgctgga agccctcaag
    20341 caaacctact gcggcccgat tggtgccgag tatatgcaca ttaccagcac cgaagaaaaa
    20401 cgctggatcc aacagcgtat cgagtctggt cgcgcgactt tcaatagcga agagaaaaaa
    20461 cgcttcctaa gcgaactgac cgccgctgaa ggccttgaac gttacctcgg tgcaaaattc
    20521 cctggcgcaa aacgcttctc gctggaaggc ggtgacgcgt taatcccgat gcttaaagag
    20581 atgattcgcc acgctggcaa cagcggcacc cgcgaagtgg ttctcgggat ggcgcaccgt
    20641 ggtcgtctga acgtgctggt gaacgtgctg ggtaaaaaac cgcaagactt gttcgacgag
    20701 tttgccggta aacataaaga acacctcggc acgggtgacg tgaaatacca catgggcttc
    20761 tcgtctgact tccagaccga tggcggcctg gtacatctgg cgctggcgtt taacccgtct
    20821 caccttgaga ttgtcagccc ggtcgttatc ggttctgttc gtgcccgtct ggacagactt
    20881 gatgagccga gcagcaacaa agtgctgcca atcaccattc acggtgacgc cgcagtgacc
    20941 gggcagggcg tggttcagga aactctgaac atgtcgaaag cgcgtggtta tgaagttggc
    21001 ggtacggtac gtatcgttat caacaaccag gttggcttca ccacctctaa cccgctggat
    21061 gcccgttcaa cgccgtactg tactgatatc ggtaagatgg ttcaggcacc gatttttcac
    21121 gttaacgcgg atgatccgga agccgttgcc tttgtgaccc gtctggcgct cgatttccgt
    21181 aacaccttta aacgtgatgt cttcatcgac ctggtgtgct accgccgtca cggccacaac
    21241 gaagccgacg agccgagcgc aacccagccg ctgatgtatc agaaaatcaa aaaacatccg
    21301 acgccgcgca aaatctatgc tgacaagctg gagcaggaaa aagtggcgac gctggaagat
    21361 gccaccgaga tggttaacct gtaccgcgat gcgctggatg ctggcgattg cgtagtggca
    21421 gagtggcgtc cgatgaacat gcactctttc acctggtcgc cgtacctcaa ccacgaatgg
    21481 gacgaagagt acccgaataa agttgagatg aagcgcctgc aggagctggc gaaacgcatc
    21541 agcacggtgc cggaagcggt tgaaatgcag tctcgcgttg ccaaaattta tggcgatcgc
    21601 caggcgatgg ctgccggtga gaaactgttc gactggggcg gcgcggaaaa cctcgcttac
    21661 gccacgctgg ttgacgaagg cattccggtt cgcctgtcgg gtgaagactc cggtcgcggt
    21721 accttcttcc accgccacgc ggtgatccac aaccagtcta acggttccac ttacacgccg
    21781 ctgcaacaca tccataacgg ccagggcgcg ttccgtgtct gggactccgt actttctgaa
    21841 gaagcagtgc tggcgtttga atacggttat gccaccgcag aaccacgcac tctgactatc
    21901 tgggaagctc agttcggtga cttcgccaac ggtgcgcagg tggttatcga ccagttcatc
    21961 tcctctggcg aacagaaatg gggccggatg tgtggtctgg tgatgttgct gccgcacggt
    22021 tacgaagggc aggggccgga gcactcctcc gcgcgtctgg aacgttatct gcaactttgt
    22081 gctgagcaaa acatgcaggt gtgcgtaccg tctaccccgg cacaggttta ccacatgctg
    22141 cgtcgtcagg cgctgcgcgg gatgcgtcgt ccgctggtcg tgatgtcgcc gaaatccctg
    22201 ctgcgtcatc cgctggcggt ttccagcctc gaagaactgg cgaacggcac cttcctgcca
    22261 gccatcggtg aaatcgacga gcttgatccg aagggcgtga agcgcgtagt gatgtgttct
    22321 ggtaaggttt attacgacct gctggaacag cgtcgtaaga acaatcaaca cgatgtcgcc
    22381 attgtgcgta tcgagcaact ctacccgttc ccgcataaag cgatgcagga agtgttgcag
    22441 cagtttgctc acgtcaagga ttttgtctgg tgccaggaag agccgctcaa tcagggcgca
    22501 tggtactgca gccagcatca tttccgtgaa gtgattccgt ttggggcttc tctgcgttat
    22561 gcaggccgcc cggcctccgc ctctccggcg gtagggtata tgtccgttca ccagaaacag
    22621 caacaagatc tggttaatga cgcgctgaac gtcgaataaa taaaggatac acaatgagta
    22681 gcgtagatat tctggtccct gacctgcctg aatccgtagc cgatgccacc gtcgcaacct
    22741 ggcataaaaa acccggtgac gcagtcgtac gtgatgaagt gctggtagaa atcgaaactg
    22801 acaaagtggt actggaagta ccggcatcag cagacggcat tctggatgcg gttctggaag
    22861 atgaaggtac aacggtaacg tctcgtcaga tccttggtcg cctgcgtgaa ggcaacagcg
    22921 ccggtaaaga aaccagcgcc aaatctgaag agaaagcgtc cactccggcg caacgccagc
    22981 aggcgtctct ggaagagcaa aacaacgatg cgttaagccc ggcgatccgt cgcctgctgg
    23041 ctgaacataa cctcgacgcc agcgccatta aaggcaccgg tgtgggtggt cgtctgaccc
    23101 gtgaagatgt ggaaaaacat ctggcgaaag ctccggcgaa agagtctgca ccggcagcgg
    23161 ctgctccggc ggcgcaaccg gctctggctg cacgtagcga aaaacgtgtc ccgatgactc
    23221 gcctgcgtaa gcgtgtggca gagcgtctgc tggaagcgaa aaactccacc gccatgctga
    23281 ccacgttcaa cgaagtcaac atgaagccga ttatggatct gcgtaagcag tacggtgaag
    23341 cgtttgaaaa acgccacggc atccgtctgg gctttatgtc cttctacgtg aaagcggtgg
    23401 ttgaagccct gaaacgttac ccggaagtga atgcttctat cgacggcgat gacgtggttt
    23461 accacaacta tttcgacgtc agcatggcgg tttctacgcc gcgcggcctg gtgacgccgg
    23521 tactgcgtga tgtcgatacc ctcggcatgg cagacatcga gaagaaaatc aaagagctgg
    23581 cagttaaagg ccgtgacggc aagctgacgg ttgaagatct gaccggtggt aacttcacca
    23641 tcaccaacgg tggtgtgttc ggttccctga tgtctacgcc gatcatcaac ccgccgcaga
    23701 gcgcaattct gggtatgcac gctatcaaag atcgtccgat ggctgtgaat ggtcaggttg
    23761 agatcctgcc gatgatgtac ctggcgctgt cctacgatca ccgtctgatc gatggtcgcg
    23821 aatccgtggg cttcctggta acgatcaaag agttgctgga agatccgacg cgtctgctgc
    23881 tggacgtgta gtagtttaag tttcacctgc actgtagacc gacgtggata gggtgtcggt
    23941 ctacggttta aaagataacg attactgaag gatggacaga acacatgaac ttacatgaat
    24001 atcaggcaaa acaactgttt gcccgctatg gcttaccagc accggtgggt tatgcctgta
    24061 ctactccgcg cgaagcagaa gaagccgctt caaaaatcgg tgccggtccg tgggtagtga
    24121 aatgtcaggt tcacgctggt ggccgcggta aagcgggcgg tgtgaaagtt gtaaacagca
    24181 aagaagacat ccgtgctttt gcagaaaact ggctgggcaa gcgtctggta acgtatcaaa
    24241 cagatgccaa tggccaaccg gttaaccaga ttctggttga agcagcgacc gatatcgcta
    24301 aagagctgta tctcggtgcc gttgtagacc gtagttcacg tcgcgtggtc tttatggcct
    24361 ccaccgaagg cggcgtggaa atcgaaaaag tggcggaaga aactccgcac ctgatccaca
    24421 aagttgcgct tgatccgctg actggcccga tgccgtatca gggacgcgag ctggcgttca
    24481 aactgggtct ggaaggtaaa ctggttcagc agttcaccaa aatcttcatg ggcctggcga
    24541 ccattttcct ggagcgcgac ctggcgctga tcgaaatcaa cccgctggtc atcaccaaac
    24601 agggcgatct gatttgcctc gacggcaaac tgggcgctga tggcaacgcc ctgttccgcc
    24661 agcctgatct gcgcgaaatg cgtgaccagt cgcaggaaga tccgcgtgaa gcacaggctg
    24721 cacagtggga actgaactac gttgcgctgg acggtaacat cggttgtatg gttaacggcg
    24781 caggcctggc gatgggtacg atggacatcg ttaaactgca cggcggcgaa ccggctaact
    24841 tcctggacgt tggcggcggc gcaaccaaag aacgtgtaac cgaagcgttc aaaatcatcc
    24901 tctctgacga caaagtgaaa gccgttctgg ttaacatctt cggcggtatc gttcgttgcg
    24961 acctgatcgc tgacggtatc atcggtgcgg tagcggaagt gggtgttaac gtaccggtcg
    25021 tggtacgtct ggaaggtaac aacgccgaac tcggcgcgaa gaaactggct gacagcggcc
    25081 tgaatattat tgcagcaaaa ggtctgacgg atgcagctca gcaggttgtt gccgcagtgg
    25141 aggggaaata atgtccattt taattgataa aaacaccaag gttatctgcc agggctttac
    25201 cggtagccag gggactttcc actcagaaca ggccattgca tacggcacta aaatggttgg
    25261 cggcgtaacc ccaggtaaag gcggtactac ccacctcggc ctgccggtgt tcaacaccgt
    25321 gcgtgaagct gttgctgcta ctggcgctac cgcttctgtt atctacgtac cagcaccgtt
    25381 ctgcaaagac tccattctgg aagccatcga cgcaggcatc aaactgatta tcaccatcac
    25441 tgaaggcatc ccgacgctgg atatgctgac cgtgaaagtg aagctggatg aagcaggcgt
    25501 tcgtatgatc ggcccgaact gcccaggcgt tatcactccg ggtgaatgca aaatcggtat
    25561 tcagcctggt cacattcaca aaccgggtaa agtgggtatc gtttcccgtt ccggtacact
    25621 gacctatgaa gcggttaaac agaccacgga ttacggtttc ggtcagtcga cctgtgtcgg
    25681 tatcggcggt gacccgatcc ctggctctaa ctttatcgac attctcgaaa tgttcgaaaa
    25741 agatccgcag accgaagcga tcgtgatgat cggtgagatc ggcggtagcg ctgaagaaga
    25801 agcagctgcg tacatcaaag agcacgttac caagccagtt gtgggttaca tcgctggtgt
    25861 gactgcgccg aaaggcaaac gtatgggcca cgcgggtgcc atcattgccg gtgggaaagg
    25921 gactgcggat gagaaattcg ctgctctgga agccgcaggc gtgaaaaccg ttcgcagcct
    25981 ggcggatatc ggtgaagcac tgaaaactgt tctgaaataa atatctgtaa taagaaatag
    26041 ccctcgccgc ttccctctac aggaatggcg aagggctgtc ggtttcgaca tggttggcca
    26101 tcgtatgatg gcctttttta tgccttaaac ggttaaccac ggtcgaaatc tgactgcagg
    26161 cttgactgca tgaccacagc cactgtggat tgccgcaacc gaccgcaagc cacgaataag
    26221 aacgacggcg aatttcgaat cgttacctga tgcactggct tcccagcgtt tgaccgtagc
    26281 gttaagtaaa tcaacaaaag cgatgttcac cgcttttcgt caagtcgtgc catttgtcag
    26341 ccaggatcgg gtctttaccc cggatattgc agcatcgcaa aaattcttag agggatatg
//
LOCUS       DABSYT020000061        26068 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.061, whole genome shotgun sequence.
ACCESSION   DABSYT020000061 DABSYT020000000
VERSION     DABSYT020000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.061"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ID725_RS15105"
                     /old_locus_tag="pgaptmp_003021"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ID725_RS15105"
                     /old_locus_tag="pgaptmp_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGV7851563.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ID725_RS15110"
                     /old_locus_tag="pgaptmp_003022"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ID725_RS15110"
                     /old_locus_tag="pgaptmp_003022"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGV7851564.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(938..1405)
                     /locus_tag="ID725_RS15115"
                     /old_locus_tag="pgaptmp_003023"
     CDS             complement(938..1405)
                     /locus_tag="ID725_RS15115"
                     /old_locus_tag="pgaptmp_003023"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGV7851565.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ID725_RS15120"
                     /old_locus_tag="pgaptmp_003024"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ID725_RS15120"
                     /old_locus_tag="pgaptmp_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGV7851566.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ID725_RS15125"
                     /old_locus_tag="pgaptmp_003025"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ID725_RS15125"
                     /old_locus_tag="pgaptmp_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGV7851567.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ID725_RS15130"
                     /old_locus_tag="pgaptmp_003026"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ID725_RS15130"
                     /old_locus_tag="pgaptmp_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGV7851568.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ID725_RS15135"
                     /old_locus_tag="pgaptmp_003027"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ID725_RS15135"
                     /old_locus_tag="pgaptmp_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGV7851569.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ID725_RS15140"
                     /old_locus_tag="pgaptmp_003028"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ID725_RS15140"
                     /old_locus_tag="pgaptmp_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGV7851570.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ID725_RS15145"
                     /old_locus_tag="pgaptmp_003029"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ID725_RS15145"
                     /old_locus_tag="pgaptmp_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGV7851571.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ID725_RS15150"
                     /old_locus_tag="pgaptmp_003030"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ID725_RS15150"
                     /old_locus_tag="pgaptmp_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGV7851572.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ID725_RS15155"
                     /old_locus_tag="pgaptmp_003031"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ID725_RS15155"
                     /old_locus_tag="pgaptmp_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGV7851573.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ID725_RS15160"
                     /old_locus_tag="pgaptmp_003032"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ID725_RS15160"
                     /old_locus_tag="pgaptmp_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGV7851574.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ID725_RS15165"
                     /old_locus_tag="pgaptmp_003033"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ID725_RS15165"
                     /old_locus_tag="pgaptmp_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGV7851575.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ID725_RS15170"
                     /old_locus_tag="pgaptmp_003034"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ID725_RS15170"
                     /old_locus_tag="pgaptmp_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGV7851576.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ID725_RS15175"
                     /old_locus_tag="pgaptmp_003035"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ID725_RS15175"
                     /old_locus_tag="pgaptmp_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGV7851577.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ID725_RS15180"
                     /old_locus_tag="pgaptmp_003036"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ID725_RS15180"
                     /old_locus_tag="pgaptmp_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGV7851578.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ID725_RS15185"
                     /old_locus_tag="pgaptmp_003037"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ID725_RS15185"
                     /old_locus_tag="pgaptmp_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGV7851579.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ID725_RS15190"
                     /old_locus_tag="pgaptmp_003038"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ID725_RS15190"
                     /old_locus_tag="pgaptmp_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGV7851580.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ID725_RS15195"
                     /old_locus_tag="pgaptmp_003039"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ID725_RS15195"
                     /old_locus_tag="pgaptmp_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGV7851581.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ID725_RS15200"
                     /old_locus_tag="pgaptmp_003040"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ID725_RS15200"
                     /old_locus_tag="pgaptmp_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGV7851582.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ID725_RS15205"
                     /old_locus_tag="pgaptmp_003041"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ID725_RS15205"
                     /old_locus_tag="pgaptmp_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGV7851583.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ID725_RS15210"
                     /old_locus_tag="pgaptmp_003042"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ID725_RS15210"
                     /old_locus_tag="pgaptmp_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGV7851584.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ID725_RS15215"
                     /old_locus_tag="pgaptmp_003043"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ID725_RS15215"
                     /old_locus_tag="pgaptmp_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGV7851585.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ID725_RS15220"
                     /old_locus_tag="pgaptmp_003044"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ID725_RS15220"
                     /old_locus_tag="pgaptmp_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGV7851586.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ID725_RS15225"
                     /old_locus_tag="pgaptmp_003045"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ID725_RS15225"
                     /old_locus_tag="pgaptmp_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGV7851587.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ID725_RS15230"
                     /old_locus_tag="pgaptmp_003046"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ID725_RS15230"
                     /old_locus_tag="pgaptmp_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGV7851588.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ID725_RS15235"
                     /old_locus_tag="pgaptmp_003047"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ID725_RS15235"
                     /old_locus_tag="pgaptmp_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGV7851589.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ID725_RS15240"
                     /old_locus_tag="pgaptmp_003048"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ID725_RS15240"
                     /old_locus_tag="pgaptmp_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGV7851590.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ID725_RS15245"
                     /old_locus_tag="pgaptmp_003049"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ID725_RS15245"
                     /old_locus_tag="pgaptmp_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGV7851591.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ID725_RS15250"
                     /old_locus_tag="pgaptmp_003050"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ID725_RS15250"
                     /old_locus_tag="pgaptmp_003050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGV7851592.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ID725_RS15255"
                     /old_locus_tag="pgaptmp_003051"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ID725_RS15255"
                     /old_locus_tag="pgaptmp_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGV7851593.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ID725_RS15260"
                     /old_locus_tag="pgaptmp_003052"
     CDS             15545..15913
                     /locus_tag="ID725_RS15260"
                     /old_locus_tag="pgaptmp_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGV7851594.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ID725_RS15265"
                     /old_locus_tag="pgaptmp_003053"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ID725_RS15265"
                     /old_locus_tag="pgaptmp_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGV7851595.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ID725_RS15270"
                     /old_locus_tag="pgaptmp_003054"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ID725_RS15270"
                     /old_locus_tag="pgaptmp_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGV7851596.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ID725_RS15275"
                     /old_locus_tag="pgaptmp_003055"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ID725_RS15275"
                     /old_locus_tag="pgaptmp_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGV7851597.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ID725_RS15280"
                     /old_locus_tag="pgaptmp_003056"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ID725_RS15280"
                     /old_locus_tag="pgaptmp_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGV7851598.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ID725_RS15285"
                     /old_locus_tag="pgaptmp_003057"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ID725_RS15285"
                     /old_locus_tag="pgaptmp_003057"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGV7851599.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ID725_RS15290"
                     /old_locus_tag="pgaptmp_003058"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ID725_RS15290"
                     /old_locus_tag="pgaptmp_003058"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGV7851600.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ID725_RS15295"
                     /old_locus_tag="pgaptmp_003059"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ID725_RS15295"
                     /old_locus_tag="pgaptmp_003059"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGV7851601.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ID725_RS15300"
                     /old_locus_tag="pgaptmp_003060"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ID725_RS15300"
                     /old_locus_tag="pgaptmp_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGV7851602.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGRP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ID725_RS15305"
                     /old_locus_tag="pgaptmp_003061"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ID725_RS15305"
                     /old_locus_tag="pgaptmp_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGV7851603.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ID725_RS15310"
                     /old_locus_tag="pgaptmp_003062"
     CDS             23092..23634
                     /locus_tag="ID725_RS15310"
                     /old_locus_tag="pgaptmp_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGV7851604.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ID725_RS15315"
                     /old_locus_tag="pgaptmp_003063"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ID725_RS15315"
                     /old_locus_tag="pgaptmp_003063"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGV7851605.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ID725_RS15320"
                     /old_locus_tag="pgaptmp_003064"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ID725_RS15320"
                     /old_locus_tag="pgaptmp_003064"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGV7851606.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ID725_RS15325"
                     /old_locus_tag="pgaptmp_003065"
     CDS             25031..25288
                     /locus_tag="ID725_RS15325"
                     /old_locus_tag="pgaptmp_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGV7851607.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ID725_RS15330"
                     /old_locus_tag="pgaptmp_003066"
     CDS             complement(25264..25818)
                     /locus_tag="ID725_RS15330"
                     /old_locus_tag="pgaptmp_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGV7851608.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6688 a         6177 c         6763 g         6440 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaatgatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttctg cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaagac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggc aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgc gacggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt cgaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cataaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttctgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgccgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacactggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcaa cggagggaag caatccagct tctggatatc ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact ggcaggatta cactcacgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgcgt agagaaaaat
    16501 aagaagggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggtc ttctgctttc aggggcttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagtg gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag cgaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggttcgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttctggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccact tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcat ccagcaatgc aagccaggtg tcgcgggaat gatgcgtacg attaatacgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gttaatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attgacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    19921 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat gccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgcct
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagta tcagcagctt
    21541 gtacggcgat gatatggtcc gtatagctca ctggctggca agacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 gcggaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggtgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagca
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtagcgc ataaagcagc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 ccgccgtcat gcccgactgg ctgccagtct gcttgaacag gggggcgctc tcgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgtagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgaga agtttttgcc ttgccaggtc caatagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc gacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    22861 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcattaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgtc cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacgcg cagcgttggc tggtgctgtt aacaccctca
    24481 agcggttaga agatggacgc ttgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgcaggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcggacggt atcccgagcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgccacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcctactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgatagcctg gcgtatcgct ttacaggaga tacgccagag cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacttgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26041 gtcgtaacaa cgaagagaaa aaatcctg
//
LOCUS       DABSYT020000062        25679 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.062, whole genome shotgun sequence.
ACCESSION   DABSYT020000062 DABSYT020000000
VERSION     DABSYT020000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.062"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            80..2308
                     /gene="crfC"
                     /locus_tag="ID725_RS15335"
                     /old_locus_tag="pgaptmp_003067"
     CDS             80..2308
                     /gene="crfC"
                     /locus_tag="ID725_RS15335"
                     /old_locus_tag="pgaptmp_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGV7851609.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2305..3183
                     /gene="rdcB"
                     /locus_tag="ID725_RS15340"
                     /old_locus_tag="pgaptmp_003068"
     CDS             2305..3183
                     /gene="rdcB"
                     /locus_tag="ID725_RS15340"
                     /old_locus_tag="pgaptmp_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGV7851610.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            3447..4949
                     /gene="proP"
                     /locus_tag="ID725_RS15345"
                     /old_locus_tag="pgaptmp_003069"
     CDS             3447..4949
                     /gene="proP"
                     /locus_tag="ID725_RS15345"
                     /old_locus_tag="pgaptmp_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGV7851611.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5061..5150
                     /gene="pmrR"
                     /locus_tag="ID725_RS15350"
                     /old_locus_tag="pgaptmp_003070"
     CDS             5061..5150
                     /gene="pmrR"
                     /locus_tag="ID725_RS15350"
                     /old_locus_tag="pgaptmp_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGV7851612.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5126..6226)
                     /gene="pmrB"
                     /locus_tag="ID725_RS15355"
                     /old_locus_tag="pgaptmp_003071"
     CDS             complement(5126..6226)
                     /gene="pmrB"
                     /locus_tag="ID725_RS15355"
                     /old_locus_tag="pgaptmp_003071"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGV7851613.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6227..6895)
                     /gene="basR"
                     /locus_tag="ID725_RS15360"
                     /old_locus_tag="pgaptmp_003072"
     CDS             complement(6227..6895)
                     /gene="basR"
                     /locus_tag="ID725_RS15360"
                     /old_locus_tag="pgaptmp_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGV7851614.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6892..8535)
                     /gene="eptA"
                     /locus_tag="ID725_RS15365"
                     /old_locus_tag="pgaptmp_003073"
     CDS             complement(6892..8535)
                     /gene="eptA"
                     /locus_tag="ID725_RS15365"
                     /old_locus_tag="pgaptmp_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGV7851615.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8639..9976)
                     /gene="adiC"
                     /locus_tag="ID725_RS15370"
                     /old_locus_tag="pgaptmp_003074"
     CDS             complement(8639..9976)
                     /gene="adiC"
                     /locus_tag="ID725_RS15370"
                     /old_locus_tag="pgaptmp_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGV7851616.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10113..10874)
                     /gene="adiY"
                     /locus_tag="ID725_RS15375"
                     /old_locus_tag="pgaptmp_003075"
     CDS             complement(10113..10874)
                     /gene="adiY"
                     /locus_tag="ID725_RS15375"
                     /old_locus_tag="pgaptmp_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGV7851617.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11199..13466)
                     /gene="adiA"
                     /locus_tag="ID725_RS15380"
                     /old_locus_tag="pgaptmp_003076"
     CDS             complement(11199..13466)
                     /gene="adiA"
                     /locus_tag="ID725_RS15380"
                     /old_locus_tag="pgaptmp_003076"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGV7851618.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13665..14573)
                     /gene="melR"
                     /locus_tag="ID725_RS15385"
                     /old_locus_tag="pgaptmp_003077"
     CDS             complement(13665..14573)
                     /gene="melR"
                     /locus_tag="ID725_RS15385"
                     /old_locus_tag="pgaptmp_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGV7851619.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14856..16211
                     /gene="melA"
                     /locus_tag="ID725_RS15390"
                     /old_locus_tag="pgaptmp_003078"
     CDS             14856..16211
                     /gene="melA"
                     /locus_tag="ID725_RS15390"
                     /old_locus_tag="pgaptmp_003078"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGV7851620.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16314..17735
                     /gene="melB"
                     /locus_tag="ID725_RS15395"
                     /old_locus_tag="pgaptmp_003079"
     CDS             16314..17735
                     /gene="melB"
                     /locus_tag="ID725_RS15395"
                     /old_locus_tag="pgaptmp_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGV7851621.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(17874..18503)
                     /gene="yjdF"
                     /locus_tag="ID725_RS15400"
                     /old_locus_tag="pgaptmp_003080"
     CDS             complement(17874..18503)
                     /gene="yjdF"
                     /locus_tag="ID725_RS15400"
                     /old_locus_tag="pgaptmp_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGV7851622.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(18626..20272)
                     /gene="fumB"
                     /locus_tag="ID725_RS15405"
                     /old_locus_tag="pgaptmp_003081"
     CDS             complement(18626..20272)
                     /gene="fumB"
                     /locus_tag="ID725_RS15405"
                     /old_locus_tag="pgaptmp_003081"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGV7851623.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            complement(20350..21690)
                     /gene="dcuB"
                     /locus_tag="ID725_RS15410"
                     /old_locus_tag="pgaptmp_003082"
     CDS             complement(20350..21690)
                     /gene="dcuB"
                     /locus_tag="ID725_RS15410"
                     /old_locus_tag="pgaptmp_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGV7851624.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(22261..22980)
                     /gene="dcuR"
                     /locus_tag="ID725_RS15415"
                     /old_locus_tag="pgaptmp_003083"
     CDS             complement(22261..22980)
                     /gene="dcuR"
                     /locus_tag="ID725_RS15415"
                     /old_locus_tag="pgaptmp_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGV7851625.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(22977..24608)
                     /gene="dcuS"
                     /locus_tag="ID725_RS15420"
                     /old_locus_tag="pgaptmp_003084"
     CDS             complement(22977..24608)
                     /gene="dcuS"
                     /locus_tag="ID725_RS15420"
                     /old_locus_tag="pgaptmp_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGV7851626.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            24789..25019
                     /gene="yjdI"
                     /locus_tag="ID725_RS15425"
                     /old_locus_tag="pgaptmp_003085"
     CDS             24789..25019
                     /gene="yjdI"
                     /locus_tag="ID725_RS15425"
                     /old_locus_tag="pgaptmp_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGV7851627.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            25031..25303
                     /gene="yjdJ"
                     /locus_tag="ID725_RS15430"
                     /old_locus_tag="pgaptmp_003086"
     CDS             25031..25303
                     /gene="yjdJ"
                     /locus_tag="ID725_RS15430"
                     /old_locus_tag="pgaptmp_003086"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGV7851628.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            25530..>25679
                     /gene="ghoS"
                     /locus_tag="ID725_RS15435"
                     /old_locus_tag="pgaptmp_003087"
     CDS             25530..>25679
                     /gene="ghoS"
                     /locus_tag="ID725_RS15435"
                     /old_locus_tag="pgaptmp_003087"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGV7851629.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQL"
BASE COUNT         6217 a         6200 c         6682 g         6580 t
ORIGIN      
        1 ttataaaagc cgaaaacaga actcaaaaac aatctggcta aataaaagta ataaaatttg
       61 ctttaaggaa gaattttcta tgtacacaca gaccctgtat gagttaagtc aggaggctga
      121 acggctgtta cagctttctc gccaacagtt gcagttactg gaaaaaatgc ctctctctgt
      181 acccggagac gatgcgccac aactggcttt accctggagt cagcctaata tcgccgagcg
      241 tcacgcgatg ctgaataatg agttgcgtaa aatttcccga ctggaaatgg tgctggcgat
      301 tgtcggtacc atgaaagcag ggaaatcaac caccattaat gccattgttg gtacggaggt
      361 actgcctaat cgcaatcgcc caatgactgc gctgccgacg cttattcgcc atacgcccgg
      421 ccaaaaggaa ccggtactgc atttttcaca tgtcgcgcca atcgattgtt taattcaaaa
      481 attacaacag cgcctgcgtg attgcgatat taagcatctg accgatgtgc tggaaataga
      541 taaagatatg cgtgcgctta tgcagcggat cgaaaatggc gtcgctttcg aaaaatatta
      601 tctgggtgcc cagcctattt ttcattgtct gaaaagtttg aatgatttgg tgcgactggc
      661 gaaggcgctg gacgtcgatt ttcctttttc tgcttacgcc gccattgagc atattcccgt
      721 gattgaagtg gagtttgtcc atctggcggg gctggaaagt tatcccggtc aattgacgtt
      781 actggatacc cccgggccaa atgaagccgg gcaaccgcat ctgcaaaaaa tgcttaacca
      841 gcagctggca cgcgcctcgg cggtactggc ggtgctggat tatacgcaac tgaaatcgat
      901 ctccgatgaa gaggtccgtg aggcgatttt ggcggtgggg caatcggtgc cgctgtatgt
      961 gctggtcaat aagttcgatc aacaggatcg taacagtgac gacgccgacc aggtgcgggc
     1021 actgatttcc gggacgctga tgaaaggctg tattacgcca cagcagatat ttccggtgtc
     1081 gtcgatgtgg ggctacctgg caaatcgggc gcgccatgag ttagccaaca acggtaagtt
     1141 accaccgcca gagcaacaac gctgggtgga agattttgcc catgccgcgc tcggcaggcg
     1201 ctggcgtcat gccgatctgg cggacctcga acatattcgt catgctgccg atcagttgtg
     1261 ggaagattcg ctgttcgccc agccaattca gatgttgctt catgccgctt acgctaacgc
     1321 ctcgttgtat gctctgcgat ctgccgcgca taaactgttg aattacgcgc agcaggcgcg
     1381 ggaatacctg gattttcgtg cgcacgggtt aaacgtcgct tgtgaacaat tgcggcaaaa
     1441 tatccaccag atcgaagaaa gtttgcagct attgcaactc aatcaggcgc aggtgagcgg
     1501 cgagattaaa catgaaatcg agctggccct gacctccgcc aaccactttc tgcgtcaaca
     1561 gcaagatgcg ctgaaggtgc agttagccgc cttgtttcag gatgattcgg agccgttaag
     1621 cgagattcgt acccgctgtg agacactgtt acagacggcg cagaacacca tcagtcgcga
     1681 ctttacgctg cgttttgccg agcttgaatc caccctttgc cgggtgttaa ccgatgttat
     1741 tcgccccatt gagcaacaag tcaaaatgga attgagcgag tcagggtttc gtcctgggtt
     1801 tcattttcct gtttttcacg gcgtagttcc ccacttcaac actcgccagc tgttcagtga
     1861 agtcatttcg cgccaggaag caacggacga gcagagcacg cgattaggcg ttgtgcgtga
     1921 gactttttcg cgctggttga atcagcctga ctggggacgg ggaaatgaga aatccccgac
     1981 agaaacggtt gattacagtg tgttgcaacg agcattaagc gcagaagtcg atctttattg
     2041 ccaacaaatg gctaaagttc tggcagagca ggtcgatgaa tctgttacgg caggcatgaa
     2101 tacttttttc gctgagttcg cttcatgttt gacggaatta cagacgcgtt tacgcgaaag
     2161 tctggctctg cgtcaacaaa atgaatcggt ggtcaggctg atgcaacagc aattgcagca
     2221 gactgtgatg actcacggct ggatttacac cgacgctcag ctgttacgcg atgatattca
     2281 aacacttttc acggcagaac gatattgacc aagacgttac tggacggccc cggtcgcgtg
     2341 ctggagtcgg tttatccccg ctttttagtg gatctggcgc agggcgatga tgcccgcctt
     2401 ccacaagccc atcagcagca gtttcgtgaa cgactgatgc aggaacttct tgcgcgcgtg
     2461 cagcttcaga catggacgaa cggcgggatg ttaaatgcgc cgcttagtct gcgtctgaca
     2521 ttggtggaaa aactggcgtc gatgctggat cccggtcatc tggcactgac gcagatcgcg
     2581 cagcatctgg cgctgctgca aaaaatggat caccgccagc actctgcttt cccggagctc
     2641 ccccagcaaa ttgccgcctt gtatgagtgg ttttcagccc gttgtcgctg gaaggaaaag
     2701 gcgttaacgc aacgaggcct actggtgcag gcgggtgaac agagcgagca aatttttacc
     2761 cgctggcgtg ctggggcgta taacgcctgg tcgttgcctg ggcgctgttt tatcgttctc
     2821 gaagagttgc gttggggggc atttggcgat gcctgtcgtc tgggaagccc gcaagcggtg
     2881 gcgttgttgc tgggtgattt gcgcgtgaaa gcgacacaac atctggcaga gagtatcaat
     2941 gcggccccga ccacgcgtca ctattaccat cagtggtttg cctcttcgac cgttccgacg
     3001 ggcggggatc atgctgattt tttaagttgg ctgggaaagt ggaccacggc agataaacaa
     3061 cccgtttgct ggtcagtgac ccaacgctgg caaactgtcg cgctggggat gccacgactc
     3121 tgttcagcgc agcgtctggc gggggcaatg ctcgaggaaa tcttctctgt aaatttggtg
     3181 taaataatca gttacatcaa tgagtcctaa acgaaatcca tgtgtgaagt tgatcacaaa
     3241 tttaaacgct ggtagggtaa aaaggtcatt aactgcccaa ttcaggcgtc aactggtttg
     3301 attgtacatt ccttaaccgg agggtgtaag caaacccgct acgcttgtta cagagattgc
     3361 atcctgcaat tcccgctccc cttttgcggc cgtcgcgctg atttttctgg cgtttgcgga
     3421 aatgggccaa ctctgcgagg aaagctatgc tgaaaaggaa aaaagtaaaa ccgattaccc
     3481 ttcgtgatgt caccattatt gatgacggta aactgcgtaa agccattacc gcagcatcac
     3541 tgggtaatgc aatggaatgg tttgattttg gtgtttatgg ttttgttgct tacgcattag
     3601 gtaaagtttt tttcccgggg gctgacccca gcgtgcagat ggttgctgca cttgccactt
     3661 tctccgttcc ctttctgatt cgaccgcttg gcggactctt ctttggtatg ttgggcgata
     3721 aatatggtcg ccagaagatc ctcgctatca ctattgtgat tatgtcgatc agtacgttct
     3781 gtattggctt aataccgtcc tacgacacga ttggtatttg ggcaccgatt ctgctgttga
     3841 tctgtaagat ggcacaaggt ttctcggtcg gcggtgaata taccggggcg tcgatatttg
     3901 ttgcggaata ctcccctgac cgtaaacgtg gctttatggg cagctggctg gacttcggtt
     3961 ctattgccgg gtttgtgctg ggtgcgggcg tggtggtgtt aatttcgacc attgtcggcg
     4021 aagcgaactt cctcgactgg ggctggcgta ttccgttctt tattgctctg ccgttaggga
     4081 ttatcgggct ttacctgcgc catgcgctgg aagaaactcc ggcgttccag cagcatgttg
     4141 ataaactgga acagggcgac cgcgaaggtt tgcaggatgg cccgaaagtc tcgtttaaag
     4201 agattgccac taaatactgg cgcagcctgt tgacatgtat tggtctggta attgccacca
     4261 acgtgactta ctacatgttg ctgacctata tgccgagtta tttgtcgcat aacctgcatt
     4321 actccgaaga ccacggggtg ctgattatta tcgccattat gatcggtatg ctgtttgtcc
     4381 agccggtgat gggcttgctg agtgaccgtt ttggccgtcg tccgtttgtg ctacttggta
     4441 gtgttgcact gtttgtgttg gcgatcccgg cgtttattct gattaacagt aacgtcatcg
     4501 gcctgatttt tgccgggtta ctgatgctgg cggtgatcct taactgcttt acgggcgtta
     4561 tggcttctac cttgccagcg atgttcccga cgcatatccg ttacagcgcg ctggcggcgg
     4621 catttaatat ttcggtgctg gttgccggtc tgacgccaac actggcggcc tggctggtcg
     4681 aaagctcgca gaatctgatg atgccagcct attacctgat ggtagtggcg gtggttggtt
     4741 taatcaccgg cgtaaccatg aaagagacgg caaatcgtcc gttgaaaggt gcaacaccgg
     4801 cggcgtcaga tatacaggaa gcgaaggaaa ttctcgtcga gcattacgat aatatcgagc
     4861 agaaaatcga tgatattgac cacgagattg ccgatttgca ggcgaaacgt acccgcctgg
     4921 tgcagcaaca tccgcgaatt gatgaataag ctgaaacgga tggcctgatg taacgctgtc
     4981 ttatcaggcc aattgaactc ttaaggttca cttaatctct gacgcgcata ctctcctcca
     5041 ggttaacgga ggagagtgca atgaaaaacc gtgtttatga aagtttaact accgtgttca
     5101 gcgtgctggt ggtcagcagc tttctttata tctggtttgc cacgttctga tctttcttca
     5161 gccgtaccca ggcccgcgtg ccggaagtct cttgccggtt ttgcaggaaa aactgcccgt
     5221 gatgcaactg tgtaatgcgg ctgacaatac ttaaccccag accaatcccg ccataacggc
     5281 tgtccatacg tacaaacgct ttactcaact ccccgcattt actctcatca atacccggtc
     5341 cttcatcttc aacggccatg accgctccgc catcttcttg cagcttaatc ataatgttgc
     5401 tgccttgcgg gctgtaacga tgggcgtttt ctaccaggtt tcgcaataac atccgcagca
     5461 gggttgcatc accctgaacg gtgatgtcgg cggcgctctc tggcaatagc agggtttgct
     5521 gtcgctggtc gagcatggta ctgagttcgt catacgaggg gagaatgaca tcttccagca
     5581 gttttacatg ttgataatta ccggaagaaa atgactgtcc ggcacgcgcc agttgcagca
     5641 gctgggagac gctctccatc atctgatcaa gccgtgccac taacggtgct acatcaatgt
     5701 gatgcgtttt cgccagcagt tccagatgca aacgcacccc cgccagtggc gttcgcagtt
     5761 cgtgcgcgac gtcagcggta aataaccttt cgttatccag cgtgctggtc aggcgactga
     5821 ccagatcgtt caacgccgaa accaccgctt cgatttcgag ggtggcgctg tgaatggcaa
     5881 tgggcgttaa gttgtcggcg gtgcgcgctt ccagctcttt ttgcagctcc gccagcgggc
     5941 gggtgatgcg gcgtaccgcc tgatagcaga taaatagcgt caggctgacc ataaagacgc
     6001 cggggacaat caggctggcg accgcctcgc ggatctcacg catgatgtgg cgatcgttgt
     6061 tgcgattgtc gcgtagcgcc tgctcaaaca gctgaatctg ctcggtactt tcatgccata
     6121 gccagaagac gctgatcagc tcaaacacca acaaaatggc cccgatggtc aatatcagcc
     6181 gttggcgcag cgatattggt cggcgcagaa aacgcatcag attcaattag ttttcctcat
     6241 tcgcgacaag catatagcca aagccgcgca cggtgcggat acgggctttg cccactttgt
     6301 cgcgcagatt gtggatatgc acttccaggg tgttggtcga gggttcattg tcccagttat
     6361 agatgtcgtt gtagagaatt tcccgatgca ccggactgcc tgctttgagc attaaccgtg
     6421 acagcagagc atattctttg ggcgtcagaa tcaactcttc accgcccatc catacctgac
     6481 ggcgacccat gttcagcgtc agattgccaa caatcagctc actttcgccc tgattattat
     6541 ggcgtcgtag cagggcgcgg atacgggcat gtaactcttc cagcgcaaaa ggcttcacca
     6601 gatagtcgtc ggcaccgaca tccagcccgg cgattttgtc ggtcagcgta tcgcgagcgg
     6661 tgaggatcag taccggcagg gtatattttt tctgccggat acgggcgaga aaatgcagtc
     6721 catcttcgtc gggtaaccct aaatccagta ccaccaggct gtaatgacct gcctcaaggc
     6781 tttgttccgc catccgcgcg gttgtcacgc catcgcacgc gtagccttcg gtttgcgccg
     6841 ccagaatcag tccctgcaat aacagcgtat cgtcttcaac aatcagaatt ttcattcact
     6901 cactctcctg caagtttgca gaatatcatc cgcagcctgg taatacttcg tctcaacgcc
     6961 agttaatccc aatagcgtgg agaataaatt gtcttgtgaa tagtgttgcg tttgcgcctg
     7021 tttttgcagg cagttctggt caacctgata ccgtttttga taatcctccg acagccacag
     7081 cagcatcggc acctgttttt ggctatccgg ggcgatggca taaggcagac cgtgcagata
     7141 gatgccattt tcacctaacg attcaccgtg gtcagaaaga taaaccaggc tggtggtaaa
     7201 tttatcctga tgttctttca gcagattaat cgctttatca acaatatagt cgacgtaaac
     7261 cagcgtgttg tcgtaagtgt tcaccagttg ctctttggta caggtctgga tctcattggt
     7321 gtcgcaggtt ggggtaaatt tcctgaactg cggcggatag cggttgtaat aggtcggacc
     7381 gtggctgccg atggtgtgta agacaatcac gccatcaccc tgcaggttat tgatgtactc
     7441 ttccagcccg tggaacagca cttcgtcata gcattcgccg ttgatgcact gatcaggcag
     7501 attcagcgcg gtgacgttct ggtgaggcac gcggtcgcag gcacctttac agccgccatc
     7561 gttgtcattc cacagcacgt tgatgcccgc tcgctgaatg atatccagca cgccttcctg
     7621 gtgctgtgcc agctcttctt tgtagtgctc tcgcggcata tccgagaaca tgcacggtac
     7681 tgaaactgcc gttgccgtgc cgcaagatgc ggtattaggg aaatagacca cgttatcttt
     7741 cgccaaccgc gggttagttt cacgcgggta gccgttgagg gagaagttct ccgcccgcga
     7801 ggtttcgccg acaatcagga tggtcaaatt tttacgtttt tcgttctgca ttaatgggtt
     7861 gcggtgcgcg tcttcaccaa ttcgcaccag cggcagattt gccagtcgct gatgggagta
     7921 ccatgaccag ctggcaacaa tgctgttaga ggggcttaag gacttcacca gctctttgtt
     7981 gttgcggaac aacgaggcgt agtctttata aaatagtgcg gcgaccagca aaatcagcag
     8041 tacagaaacc agaatattgg ctccacggaa aagaacactg cgcagacgcg aggtggcagg
     8101 tttgatttta atccagcagg caatcagcgc agcaagcacg ccgctgaatc ccagcgttaa
     8161 taacatttgc ggtgtcatca gcgcatagct ttctgccgga gtggtatcaa taatattggc
     8221 aatcatcgag cggtcgatga cgatgccgta agtcattatg aaatattgtg cagccgcgcc
     8281 aaccagaata aacaggcagg ccagcggtcg attaagccat aagaaagagc ttagtgtcag
     8341 gacaatatta atcacgctga aggctacgac cggcatcgac aagaaaacca gtacgttatg
     8401 cagcgagtcc agcggcagcg cctgcaacac ctgtttaaaa aaggcaatat tcaggcagat
     8461 agagatataa aaagcggcca acaatagcca ggcgagtaaa ttcaaagagg gtctttttag
     8521 taggcgcttc aacatcacgg tgtttccatc gaacaaagtg cgcatatgct cgcaaagcaa
     8581 aattaagcca accttaagtt cttaaggttg gcttttatct ttgctggatt aaggggaatt
     8641 aatctttgct tattggtgca tctaagggat acgggttttt atgcagccgg ttgtaattca
     8701 gcgcatacat ggcggtgatg accatcaggg tgacaaatga ccacataacc tctttcgccc
     8761 cggaccccac cacggcccag atgcagtaga ggaaggcaat ggtagtaacc gccagatatg
     8821 ccgggcgtgc tttaccaaag tgaccgtgtc cgagcagcag taacgccgca caggtgtaaa
     8881 gatatggcac cagtgtaaag atgaccgaca cggaagaaac cagaccgaat tctttggtcg
     8941 cgtttggtga aatgctgctg agctggaaga tggtcatcaa aataccgacg ataatcagcc
     9001 ccgccactgg cgtacccgct ttatttacac gggcaaaaat cggtgggaac agtccgtcat
     9061 cggcagccgc tttcgccgtt tgacccgcca gcaatgtcca gccgcccagt gaacctaagc
     9121 aacccgcagc tgcgcagaag gaaacgatgg ccccggcggt gtcacccaac gccatccgtg
     9181 cggcatcacc gaatggcgag gcagaaacgc gcagtgcggc attagggatc atccccataa
     9241 tcgcggtggt ggagagcaca tagcaaacgg cggcaatcaa taccccacca atggtggcta
     9301 tagggacgtt gcgtttcggg tttttcacca cacctgcggc aacggaggca ctttccacac
     9361 cgatgaacga ccacagcgta acgttaaggg tactttgaat tgcaccgaag gtgcccaggc
     9421 cgctgacgtt ccatgccgcc atataggttt caccacgaaa ccagaaccag ccaaatacgg
     9481 caatcccgac gatggggatc agcgccagca cggtggcaac ggcctgcaca cgggtgatca
     9541 ttttcggacc gacaatgttc agcaggacga agatccacag caccacgacg caggtgatgg
     9601 ttaataccaa tggatctttc agaatcggga agaagtaact taaatatcct acgccaatga
     9661 ccaccatggc gatattgccg atccagcagg ccagccagta gaggacgttg gtttgataac
     9721 cgagaaacgg gccaaagcag cggcgggcgt aagcgtaaga accaccagga cttgggtcga
     9781 ggaacgacat tttggcgtat accatcgaga gccccagcgc accgataatc gtcaccaacc
     9841 atccataaat ggcaatcccg ccggtggagg ccaggtttgc aggtaacaga aaaacacctg
     9901 accccataat attccccgac accatcaggg tgacggggat taagcccact ttgtgagcat
     9961 cagcatccga agacataatt aaactcctgc gaaggcgagc ttcgtgacaa taaattacgt
    10021 catatcatac gcctgcattc gatagttgaa ttattatcga ccgggttatc gctgatgccg
    10081 ttattaatat agctcttcgt taaggttaaa aatcaggcga cagtgcgttc tctgtgctga
    10141 ctaacataat gcagcggtgt cataccgtag aagtctttaa agacagaaat aaagtacgac
    10201 gtactgttgt agccgcagga ctgtgatacc tgagagatat ttttaccgtc catcattaat
    10261 tcatttacgg cataacgcat gcggcaggtg gtgattattt ggctataact ggtgttttcg
    10321 cttttcagct ttttctttaa cagacttggg ctaagacata aacaactggc taccatacta
    10381 agattccagt ctttgtgaat atcgctttca ataatttgat aaacgctgtc acttacacaa
    10441 ttacgtaaca tatacatcat taatgaaagg aattttttac tgtcgagaaa acgggatagc
    10501 accgtgaata ataatgcgcg tgttcgttcc ttttctgact cagtttctgc gggcatcatg
    10561 ctgtgttgcg cggcaacgcg aaagacgtct ggtgtcaggc atggcagggt gagaatgggc
    10621 gtagcgctac gttgccatac tggtatttgc gagagatctt tattcagaaa ccggaggtaa
    10681 tctttgatga tgtcgtgact aatatgagca accaaagtgt tgttaagaga ggagacgtcg
    10741 ataatattat tttcacaatt taataacgcc atatggttag ctttaaggct aataggttct
    10801 ttcccattaa cccttatcca gacatctttt tcagtcaata aaacaataca aggttggtcg
    10861 ctgcaaatcc tcatacatgt actcctgagt gcgaataaaa gaccagttct cgctaaagca
    10921 aagcgatacc gggaaatggt tatataattg tatgatgaat ataaacatgc ataaaaataa
    10981 cgagttttac taatattaca aatttttata aaaatttctc atgtacatat ttttttacag
    11041 ggtaaataac aggcaaaaaa aagcgcgggg tggggccgcg caaaaagtat gacatttttg
    11101 tttgcagcaa tttttatgaa aacgttgcca tactcaaaag gcctaaggcc aggcggcgta
    11161 atgttattta aacaattacg ccttcagcgg aatagtggtt acgctttcac acacataacg
    11221 tggtaaatac cgtcaataat ttcagtccct tcagtttcgt gttcaaatcc agggaaatga
    11281 tggtcccagg attgcagcga gcgtaaataa cttacttgcg gactgttttt atcgccgaag
    11341 ttttcaccag acagcagcat cgggattcct ggcggatacg ggataactga gtttgccgcg
    11401 atacgtcctg gcagattttc aatggatacc agttcgacat tgttgtcgac aatcgcgttg
    11461 tacgcttcac gcggggtgat ttccgccacc ggcaggccgg aataggcttc gttcaaccgt
    11521 gcgccagggt tgttttcttt cagccaggca aacatggtgt cacccagatc gtgaatcccc
    11581 atgttcgcgt aagtgtcagg atattgttca acaagttccg gcatcacctg cgccagcggt
    11641 gtgttggcgt catagtggcg tttgaaggag caaagggtgt taaccagagt cccccatttc
    11701 ccacgggtta cgcccataga gaacaggaac ataatttgga agtcagtggt gcgggtaggc
    11761 acaatgccgt ggcgaccaag ccaggcagtg accagcgccg ccggaacacc ggtttcttcc
    11821 agttcaccat cttcacccat tcccggagca aggatgctga ctttaatcgg gtcgagcata
    11881 ctccagttat ccggaatatc tttgaagccg tgccagcttt cgcccggatg cattacccag
    11941 cagtcctgaa cggtggtcag cagtttggtt ggtgcgtcag caaagtcata ggttttgccg
    12001 gtttgtgggt cggtgacgac ttctttgttc cacggtttga agaaccagct accgtcagcg
    12061 gcgaactctt tatacagccg cgccatcgcc tgacggaaat caaccgcttc gtcgatcact
    12121 tcctgcgtca gtgacaggcc gctgttgccg tccatcatcg acaccgccac gtcgttggat
    12181 gcgcagatgg catacagcgg ggaggtggtg gcatgcatca tgtaggcctg gttgaagcgg
    12241 gagaagttaa tcgccccacg accttcacgt acatgaatat aagaggcctg tgaaagcgca
    12301 ttcagcagtt tgtgggtgga gtgggtggcg aaaacggtag gaccgttgtg atcgccaggt
    12361 tcgccgcgca tggcatagtg atcggcatag atcgggttga aacgtgcata gccgtaccag
    12421 gcttcgtcaa agtgcagacg atcggaggtt ttttccagca gatcctgcgc ttctttagcg
    12481 ttataacaca cgccgtcata ggtgcagttg gtcaccacgc agtaagacgg tttttgcccg
    12541 gctttgtctt tagtcagcgg gctttcactg attttcttct gcaaggtttc aggttgcatt
    12601 tcctgcggat agattggccc gataatgccg tagcggttgc ggcttggcac catgtagacc
    12661 ggtttcgcgc ctgtcagcat caaaccttgt tcgatggatt tatggcagtt acggtcaacg
    12721 accacgacat cgttatcggt catgcaagcc tgcatgatgg tgcggttaga gccggaagta
    12781 ccgacgacta ccgaccagga gcgatcggca ccaaatacgc gtgcggcata tttttcgctt
    12841 tcgccaaatg cgccagtatg gtcaagcaaa gaaccgaggg aagttcgttc gatgcccatg
    12901 tcggtgcgga acagattttc accgtagtag tcatggtaga aacgtccggc gggtgttttg
    12961 gtaaaaccaa cgccgccctg gtggcctggc gctgcccagg aatattcatg gatgtcgcta
    13021 tatttcatca gcgcgctgaa cagtggcggc aacagctgct ggcggtagcg ggtcatcgcg
    13081 gcaacggcgc gtccggcgat aaagtcggcg gtatcttcca gaatccaggc gaattcatcg
    13141 acaagctcca gcagatcccg gtccattgcg gcgagggctt tttcccgatc gcccaacagg
    13201 aagaccggca cgttttgttg gcgctcatga agcttaccga tcaattgtct gacgttttga
    13261 tgttcgtccg gatgttccat ttgatagctg aacatcaggc agtcaatggc ttcgtttgaa
    13321 gagagaatgg caaaaccatc atcaaaggag gtggatttaa tcacggtaac attttgctgg
    13381 cttaaagcat ctgccagacg ctcaacagcg ttaccgaccc aggtgtcttg atggagaaac
    13441 tcgctttcaa caattaatac tttcatcatt gcttacccgg ttatgaagga atcttcgttg
    13501 cgggcaagta tcttccggcc ttttcgggct gtaaagcgcg tgaaaaacaa acatttttga
    13561 aatatttgtt tttataaaca atatgttatg tgattatttt aaattatttt cactgcttat
    13621 tatcgacggc taaactattt ttttggctga tactgatatc gtctttagcc gggaaacgtc
    13681 tggcggcgct gttggctaag tttgcggtat tgttgcggcg acatgccgac atatttgccg
    13741 aacgtgctgt aaaaacgact acttgaacga aagcctgccg tcagggcaat atcgagaata
    13801 cttttatcgg tatcgctcag taacgcgcga acgtggttga tgcgcatcgc ggtaatgtac
    13861 tgtttcatcg tcaattgcat gacccgctgg aatatcccca ttgcatagtt ggcgttaagt
    13921 ttgacgtgct cagccacatc gttgatggtc agcgcctggt catagttttc ggcaataaag
    13981 cccagcatct ggctaacata aaattgcgca tggcgcgaga cgctgttttt gtgtgtgcgc
    14041 gaggttttat tgaccagaat cggttcccag ccagagaggc taaatcgctt gagcatcagg
    14101 ccaatttcat caatggcgag ctggcgaatt tgctcattcg gactgtttaa ttcctgctgc
    14161 cagcggcgca cttcaaacgg gctaagttgc tgtgtagcaa gtgatttgat caccatgccg
    14221 tgagtgacgt ggttaatcag gtctttatcc agcggccagg agagaaacag atgcatcggc
    14281 agattaaaaa tcgccatgct ctgacaggtt ccggtatctg ttagttggtg cggtatacag
    14341 gcccagaaca gcgtgatatg tccctgattg atattcactt tttcattgtt gatcaggtat
    14401 tccacatcgc catcgaaagg cacattcact tcgacctgac catgccagtg gctggtgggc
    14461 atgatatgcg gtgcgcgaaa ctcaatctcc atccgctggt attccgaata cagcgacagc
    14521 gggctgcggg tctgtttttc gtcgctgctg cacataaacg tatctgtatt catggatggc
    14581 tctctttcct ggaatatcag aattatggca ggagtgaggg aggatgactg cgagtgggag
    14641 cacggttttc accctcttcc cagaggggcg aggggagtgt ccgagcatca tgaggccgaa
    14701 aactctgctt ttcaggtaaa ttattcccat aaactcagat ttactgctgc ttcacgcagg
    14761 atctgagttt atgggaatgc tcaacctgga agccggaggt tttctgcaga ttcgcctgcc
    14821 atgatgaagt tattcaagca agccaggaga tctgcatgat gtctgcaccc aaaattacat
    14881 ttatcggcgc tggttcgacg attttcgtta aaaatattct tggtgatgtg ttccatcgcg
    14941 aggcgctgaa aacggcgcat attgccctga tggacattga ccccacccgc ctggaagagt
    15001 cgcatattgt ggtgcgtaag ctgatggatt cagcaggggc cagcggcaaa atcacctgcc
    15061 acacccaaca gaaagaagcc ttacaggatg ccgattttgt cgtggtggca tttcagattg
    15121 gcggttatgc accttgcacg gtgactgatt tcgaggtctg taagcggcat ggtctggaac
    15181 aaaccattgc cgatacgttg gggccgggcg gtattatgcg cgcgctacgt accattccgc
    15241 atctgtggca aatttgcgag gacatgacgg aagtctgccc cgatgccacc atgctcaact
    15301 atgttaaccc aatggcgatg aatacctggg cgatgtatgc ccgctatccg catatcaaac
    15361 aggtcgggct gtgccattcg gtgcagggaa cggcggaaga gctggcgcgt gacctcaata
    15421 tcgacccggc tacgctgcgt taccgttgcg caggtatcaa ccatatggcg ttttacctgg
    15481 agctggagcg caaaaccgcc gacggcagtt acgtgaatct ctacccggaa ctgctggcgg
    15541 cttatgacgc agggcaggca ccgaagccga atattcacgg caatactcgc tgccagaata
    15601 ttgtgcgcta tgaaatgttc aaaaagctgg gctatttcgt cacggaatcg tcagaacatt
    15661 ttgctgagta cacaccgtgg tttattaagc caggtcgtga ggatttgatt gagcgttata
    15721 aagtaccgct ggatgagtac ccgaaacgct gcgtcgagca gctggcgaac tggcataaag
    15781 agctggagga gtataaaaac gcctcccgga ttgatattaa accgtcacgg gaatatgcca
    15841 gcacaatcat gaacgctatc tggacgggcg agccgagtgt gatttacggc aacgtccgta
    15901 acgatggttt gattgataac ctgccacaag gatgttgcgt ggaagtggcc tgtctggttg
    15961 atgctaatgg cattcagccg accaaagtcg gtacgctacc ttcgcatctg gccgccctga
    16021 tgcaaaccaa catcaacgta cagacgctgc tgaccgaagc tattcttacg gaaaatcgcg
    16081 accgtgttta ccacgctgcg atgatggacc cgcatactgc cgccgtgctg ggcattgacg
    16141 aaatatatgc tcttgttgac gacctgattg ccgcccacgg cgactggctg ccaggctggt
    16201 tgcaccgtta aaacgcgact aaacgctact gcgccggggg atttattccg gcgcacacct
    16261 ctgacgatac caataacaga aggcgggcgt tggtaacagc gacccgatac cctatgagca
    16321 tttcaatgac tacaaaactc agttatggat ttggagcgtt cgggaaggat tttgcgatcg
    16381 gcattgtgta tatgtacctc atgtattact acaccgatgt cgtcgggctg tctgtgggtt
    16441 tggtcggtac tttgtttctg gtggcgagga tctgggatgc tattaacgat ccgattatgg
    16501 gatggattgt aaatgctacg cgatcgcgat ggggtaagtt caaaccctgg atcctgatcg
    16561 gtacgttggc aaactctgta atcttatttc tcctctttag tgcgcatctg tttgaaggta
    16621 ctactcagat tgcctttgtt tgcgtgacct acatcctctg gggcatgact tacaccatta
    16681 tggatattcc cttctggtcg ctggttccaa ccatcacgct cgataaacgt gagcgtgaac
    16741 aactggttcc ttatccgcgt ttttttgcca gtctggcggg ctttgttacg gcaggtgtga
    16801 cgctaccatt tgttaattat gtcggcggtg gcgatcgggg atttggcttt cagatgttca
    16861 cactggtact gatcgccttt tttattgttt caaccatcat cactctgcgc aatgtgcatg
    16921 aagtcttttc gtcagacaat caaccgtctg ctgaaggaag ccatctgaca cttaaagcca
    16981 tcgttgcgct tatttataaa aacgatcagc tttcatgcct cttgggtatg gctcttgctt
    17041 ataatgtagc cagcaacatt attaccggct ttgctatcta ttatttctca tatgttatcg
    17101 gtgatgcgga tttgttcccc tattatctgt cgtatgcggg agctgctaac ctggtgacgt
    17161 tagtattctt cccacgctta gttaaatcat tatcccgacg cattttatgg gccggagcat
    17221 ctattcttcc ggtgttaagc tgtggtgttc tcctgttaat ggcattaatg agctatcaca
    17281 acgtcgtcct cattgtgatt gcgggtattt tgctgaatgt gggaacggcg cttttctggg
    17341 tattacaggt catcatggtg gcagataccg ttgattacgg tgaatataaa ctgcacgtac
    17401 gctgtgaaag catcgcttac tccgtgcaga ctatggtggt gaagggcggt tcagcctttg
    17461 cggctttttt cattgcggtt gtgttaggga tgattggcta tgtaccgaat gttgaacagt
    17521 ctacgcaagc cctattaggt atgcagttta ttatgattgc tctaccgact ctgtttttca
    17581 tggtaacgct gattctctac ttccgtttct atcgcctcaa tggcgacacg ctgcgcagga
    17641 tccagattca tctgctggat aaatatcgca aaataccgcc cgagcctgtt catgctgata
    17701 ttccggtcgg tgcagtgagt gatgtgaaag cctgacgtgg acaatagcta acgaaaatgg
    17761 cctgatgcga tatgtttatc aggccacgtc ttaatggaat gctttggatt tgcaaatttt
    17821 gtagaccgga taaggaattc acgccgcatc cggcatcaac aaagtgcacg ttgttatccg
    17881 gttatcaagc caaagcgccg tagctgacgg caatgaaagc gagcgaggaa tatcaccgtg
    17941 gttaatgcgc caagcagcgc gcagaacata tcggattgcg tgtcccactg gtcgccctgg
    18001 gtgccgagaa aatcatccgc tccctggccc attgccagcg ccgcccacca ctcgattaat
    18061 tcatacatcg cgcttatcgc cagcgccacg caacagacca gaaacgccac cattttacgt
    18121 ccgcgaacgt acatcccgcg cacaaggatc tctcgtgcca ccagtgcagg caccagcccc
    18181 tggaaaaaat gccccagctt gtcatacgga ttacggctca accccagcca ttcctgcacc
    18241 tcaaaaccga tggggacttt cgcgtaggtg tattgcccgc cgaccatcag gatgatggcg
    18301 tgaaagaaaa tgagcgtata gagcagcggc gttaacggat aacgtttagc ggtggcaagc
    18361 agtagcggca cgacaataat caccggtgtc acttccatca gccaggtgag tttgtcatgg
    18421 gccgaaatgc cggtataaat caggattaac gttagcgcca gtgcgccggt gttaagaatt
    18481 aacggcttga gtgtgcgggt catggtgagt tcgcaagtca gggaaaatac tgactattca
    18541 ccagcaagcg tgaaattaca atcgcaaaag aggtggccca gggggatcac ctggcagcat
    18601 gctgccaggc gctgggccga agaggttact tagtgcagtt cgcgcactgt ttgttgatga
    18661 tttgctggaa gaagtcgtta cctttgtcat cgaccaggat aaacgccggg aaatcttcta
    18721 cttcgatttt ccagatagct tccataccca gctccggata agcgacgcat tccagatgct
    18781 tgatgctctg ctgcgccagt accgccgccg gaccgccgat gctaccgagg tagaagccgc
    18841 cgtgtttatg acacgcgtcg gtaacctgct gactgcggtt acctttcgct agcatgatca
    18901 tgctgccgcc gtgggattgc agcagatcca cataggagtc catacgacct gcggtggttg
    18961 ggccaagtga gcctgatgga taaccggcag gggttttcgc cggacccgcg tagtagatcg
    19021 ggtgatcttt gatgtactgc ggaagttctt taccggcgtc aatcagctct ttcagcttgg
    19081 cgtgtgcaat atctcggccc acgataatgg tgccggtgag cgacaaacga gtggaaaccg
    19141 gatattgcga aagctgggcg aggatctctt tcatcgggcg gttaaggtcg accttcaccg
    19201 cttcgccttc accggcctgg cgcagttctt gtggaatgta ctgacctgga tggtgttcca
    19261 gtttttcgat ccagatacct tcgcggttga ttttcgcttt aatgttacgg tcagcggagc
    19321 aggagacgcc catgcctacc gggcaggatg cgccgtgacg tggcagacgg ataacgcgaa
    19381 tgtcgtgcgc gaagtattta ccgccaaact gcgcgccaag accgagtttc tgggcctctt
    19441 ccagcagttc ctgttccagc tggacatcgc ggaacgcctg accatgttcg ttcccttccg
    19501 tcggcagttc atcgtaatag tgagcgcttg ctaacttaac ggttttcagg ttggtttcca
    19561 cagacgtacc gccaatcaca aacgcgatat ggtacggcgg gcaggctgca gtaccgaggg
    19621 tacgcatttt ctcgacgagg aagtttttca gtttgccggg agtcagcagg gctttggttt
    19681 cctggtagag ataggttttg ttggcagagc cgccgccttt cgcaacgcaa aggaatttgt
    19741 actcatcgcc atctaccgcg tacaggtcga tttgcgcagg caggttagtg ccggtgttga
    19801 cctctttgta catgtccagc gccgcgtttt gcgagtagcg caggttatct tcgatatagg
    19861 tgttatagac gcctttcgac agcgcttctt catcaccgcc accggtccac acgcgctggc
    19921 cttttttacc gacgatgatc gcggtgccgg tatcctggca ggtcggcagc acgcctttgg
    19981 cggcgatttc ggagtttctt aagaattgca gtgccacgta cttgtcgttt tcgctggctt
    20041 ctggatcgtg aagaatagcc gcaacctgtt tctggtgtgc cggacggagc atgaaagaag
    20101 cgtcgtggaa ggcttgctgc gccagcaggg tcagggcttc tggttccact ttcaggatgg
    20161 tttcgccgtc gaagtcggca acgctaacgt aatcggaagt gagtagatag tattcggtat
    20221 tgtctttccc catcgggaaa ggtgcctggt agataaaggg tttgtttgac atagcttcca
    20281 gcctgtaact ctgtatttgt tattcgaaaa tggcgtgccg cgtagtaacg ggcggcacgc
    20341 aaagtgcatt tataagaacc cgtacatcgc ggcgaagatc cagccgaaga cgcacgatac
    20401 gctcacacca atcaaccccg gcagaataaa gctgtggttg atgacgaagc gaccgatgtg
    20461 ggtggtgccg gaacggtcaa actgaatcgc tgccagatcg ctcggataag tcggcaggat
    20521 gtaataaccg tagcaagccg gtgctgaagc cacgatgtat gccggatcaa cgccgatcgc
    20581 tagcgcgacc ggaacaatcg ccgccagcgc cgcagcctga gagtttacaa acttggaaac
    20641 cagcagcaga acaatggcat aggcccacgg atactctttc accatttcac ccagtacgcc
    20701 ctgaatttca gacatatgcg caccgaacat ggtttctgcc atccatgcga taccgtacac
    20761 cgccacgatg gcgatcatac cggaacggaa gacttcgttt tttgagatag acgcgggatt
    20821 ggttttggtc aggataataa tcagcgcccc ggtcagcagc ataaacatct gaataaccag
    20881 taccatcgac agcggtttgc cgccgaagga tggacgcagg tccgaatcag caccaagaag
    20941 tgcgactaca gcgattgccc cgaggaaaat ccacattgcc agccagttgc ttttcggcag
    21001 ttttttatcc agcagcgtcg cggtatcacc gtaaacatac tcacggtttt ccggtacgga
    21061 gatgaatttc tggaactctt cgtctttatc cagatcttta ccgcggaacc agctgaagat
    21121 accgatcgcc aggataccga ttaacgtcga tggaatggtg attgccagca ggtcgaggaa
    21181 ctcaagatgg cgaccatcaa aggtgacatt acccagcatt gcaaccagag acacgaccgc
    21241 aaccgacacc ggactggcga taatccccat ctgtgcaccg atagaacttg ccgccatcgg
    21301 acgttccgga cggatgttgt tcttaatggc gacgtcgtag atgatcggca gaatggtgta
    21361 aaccacatga cccgtaccgc aaagaatggt cagcgtacag gtcacaaacg gcgcgacaat
    21421 tgagacatat ttcgggttgc ggcgcagcag cttctcggca atttgcagca tgacatcaag
    21481 accgcccgaa gcttgcaagg tcgccgatgc cgccaccacc gcaatgataa ccagcatgac
    21541 atcaactggt ggtttacctg gctgaaggtg gaagacgaag accagaatga ccagaccgat
    21601 accgcctaat aaacccagcg cgataccacc ctttctggca ccataaaaca gacatatcag
    21661 tattatgata agttggatag taaataacat gtgtgaaccc tcgcgataat cctatttaaa
    21721 tttttgctga atagatcaca gtcacgttct gttttgtatg aactgttttc agagcctgac
    21781 taaatatccg tttggtcgtt accggcttta gcaaatacct cacagtgaat attggctgat
    21841 taaagcagcg gcaaatttat gcactgtgat aagcggcttt ttcagagaga aaaactctct
    21901 ggcgatgggt ttaaagtttg tactgcgcag atgttaatgt tttgtttgct gttatcttat
    21961 tgatatttat cggtctattt tttgttctca gggcagatat cattaggttt atagattaat
    22021 ctgatctacc catttgtggg taaaaataca cataatgcgg gtgacataat agttaattaa
    22081 cttttgttag cgttttgaaa ttaaaaacac cgttcacctg aagagatatt aatttttagc
    22141 gatgatggag ggataattat atttgatctg gcacaagttt tactgatgaa ggatgtaact
    22201 tgtaccaggg gtattttgca ttacggtaat aattattact tacagataac cagcgatccg
    22261 ttattggcaa tattgtttca gtagtgagta gtgttctgcc tgaatacggt aacggtaaac
    22321 tggacgcccc gtgacgccat aatggatact ggtgaacaag atgtggcagt tgaccagcca
    22381 gataaggtat ttacggcagg aaacacgcga aatgttaacc tcgttggcta gctcgtcggt
    22441 tgaaaactca tagtcctgat gcgcgtcaat ccactggcac agtgtgcgta acgtctgcgg
    22501 cgttaagcct tttggcaagc gacgaggatc ctgctcgttg gagctgctgc cgtggattag
    22561 ctgatcaagc tcggcctggt cataatactg atgtttttcc agcgccattt tcttttgccg
    22621 ccagccggtg agcgcctctt caaagcggga agcctggaag ggtttgatca ggtaatccac
    22681 gacaccgtaa tgcagcgaat ctttaatggt tgccgcatcg gctgcggagg agatgacaat
    22741 cacatcactt ttgcaacgcg cgttatgcag gacaggcagt aaatcgagcc cgttctcttt
    22801 ttgcatatag atatcgagca atatcaggtc gataggcgta tcgctattga agataatctc
    22861 tttggctttc tccagcgtcg aggctgttcc acagcattga aagcctggga tttgtgctac
    22921 gtatcggcga ttcagctccg cgaccattgc gtcgtcatcg ataattaata cattgatcat
    22981 ctgttcgacc tctccccgtc ccagggtatc tggacaaaaa attgtgtgaa aatcccgggt
    23041 tccgattcca cggcgatgct gccgccgaga ttttctacct gttgtttgac aagtgctaaa
    23101 ccgacgcctc gctcgcttcc ttttgtcgag acacctttgt caaaaatgtg atcgattttg
    23161 tcgggtgcga tccccggtcc atcatcgtta acttcgcagt gcagccagcc gtgacggtag
    23221 tgcaatgtta cgctaatttc gccaccgggt tccggcccta atgcttccag cgcgttttct
    23281 atcagatttc ccaatgtggt aatcagcgtc gcgacctggt cctcactgcc gctgtctggc
    23341 agctggcttt cactgtttaa aatcagcgta tggcctaaat cggtcgcgcg gttaatcttg
    23401 ctgattaaaa aaccagcgat aaccggagat ttgatcttac ccagcagaga gccaatctct
    23461 tcctgatagt tattggctgt tttgagaatg taatcttcca actgcttata actcttcaga
    23521 tgcaataatc cgagaatcac atgcaattta ttcataaatt cgtgggatcg ttcacgaagt
    23581 gcatcagcat agttgaccag accgtcgagt cgctgcatca gtttacgaac ttcagttttg
    23641 tccctgaagg ttgaaatggc accgatgata acgccattac tgcgcaccgg aacggtgttg
    23701 atcagtaata gccggtcttt aatcgtaatc tcttcgtcgc ggcgcggcgt accgtcgcgt
    23761 aacacttccg agacatctac cacctgtgac catgagtggc tgagcgtcga cagtttctca
    23821 tcgtcctgcg acttacggta attcagcaat tcttgtgcgg catcgttgat cagcgtgacc
    23881 tcgccgcgat cgtccacggc aacgacgcct tctttgatag actgcaacat ggcctggcgt
    23941 tgctcaaaca gcgtggagat ttcgtagggt tccaggccga aaaggatttt tttcagtacc
    24001 ttaaccagaa tgcaggtgcc aatcagtccg accagcatgc caaataatac cgaccagata
    24061 atgctccagc gactgtcatt gatctgttgg gtcacgcggc ttaactcaag gccgatcgcc
    24121 accacgccaa tttgtttatg attttcatcg tagatggggg taaatacgcg taaagcctgc
    24181 gccagaaaac cgcgattgat agcgacattt tcttcgccat tcagcgcttt aaggatgtca
    24241 tcacctttaa atggctgacc aatacgctgg gcttcaggat gcgagtagcg aagactttgc
    24301 atatcggtaa cgacaataaa cagcagatcg ttgcgtttgc gcacggcttc cgcgatggcc
    24361 tggatgccgc tctcctgcgg ttttttctgc aagccctgac ggatttccgg cgagtcggcg
    24421 agagtacgcg ccactgccag tgccttgttg gctagcccgt ctcgcgtcat atcactgatt
    24481 tgcgagaagt aaatcagatg caccaccaat agcaccgaga acagtaccgc gctgaccatt
    24541 aagatcactg tggtactcaa tttcatcgga cgtttgcgta acatgcggta gggcaatgaa
    24601 tgtctcatca gcttccttgt gtgacaaatt tcttaagcat tatctctgat gaggcgggta
    24661 attcaaaggg agtaagaatg attggctata taggggaaga gactctggca acggaaactg
    24721 ccagtgctgt atgaagattc cagggctatg cttatagtga aaaccatact gatgagagag
    24781 ggaaggtcat ggatcaggcg ctactggacg ggggttatcg ctgttatacc ggcgaaaaga
    24841 tcgatgtcta tttcaacact gcgatatgtc agcattctgg caattgcgta cgtggcaacg
    24901 gcaagttatt taatctcaaa cgaaagccgt ggatcatgcc ggatgaagtc gacgttgcca
    24961 ctgtggttaa agtgattgat acgtgcccga gtggtgcgct gaaataccgt cataaataag
    25021 cgagggtaaa atggaaatac gcgaaggcca caataaattt tacattaatg acgaacaagg
    25081 caagcaaatc gctgaaattg tctttgtgcc gaccggagag aatttagcga ttatcgaaca
    25141 taccgatgtc gatgaaagcc tgaaagggca agggattggt aaacagctgg ttgcgaaagt
    25201 cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca ttatgtccat ttgcgaaaca
    25261 cgaatttgat aaaacgcggg agtatgatga tattcgcagt tgatggaaga gtacagattc
    25321 acgatatttt tcatgctctc cgcggtgtga tgcaggagag catctgaagg gtagggggat
    25381 gcacaaagaa tgggcagaga gcgcgttttt ttgtcccaag tcatcccctt tactgagcaa
    25441 aaaaaagaat atctcctata tgagaatcat caatcggggt taataaattt tgcgtcccca
    25501 gagtgttcaa tatttatagg agtcatatta tggaaggtaa aaacaagttc aatacttatg
    25561 ttgtttcttt tgattatcca tcatcttatt cctcagtgtt cttaaggtta agatcattga
    25621 tgtatgatat gaatttctct tctatcgtgg ctgatgaata tgggatacca cgacaattg
//
LOCUS       DABSYT020000063        25311 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.063, whole genome shotgun sequence.
ACCESSION   DABSYT020000063 DABSYT020000000
VERSION     DABSYT020000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.063"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..866)
                     /locus_tag="ID725_RS15440"
                     /old_locus_tag="pgaptmp_003088"
     CDS             complement(<1..866)
                     /locus_tag="ID725_RS15440"
                     /old_locus_tag="pgaptmp_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HGV7851630.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKGKVNSIYGKSIDYSTM
                     RHRDIIIAKIDLFIQRITHNLWTAREKNVTLIKQINDLKMCVNEYIGDCTDEELNDRE
                     FIASVVDRAIFHFAINSICNPEDNKDATLIERCTFDVETKNGLPSTVQLFYEESKDNE
                     PLANIHLQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSVYSDFAGRVNINEYN
                     DNYLKAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDILSKM
                     RDAAPKLIDFAKS"
     gene            1178..1693
                     /gene="zur"
                     /locus_tag="ID725_RS15445"
                     /old_locus_tag="pgaptmp_003089"
     CDS             1178..1693
                     /gene="zur"
                     /locus_tag="ID725_RS15445"
                     /old_locus_tag="pgaptmp_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGV7851631.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(1735..1944)
                     /gene="yjbJ"
                     /locus_tag="ID725_RS15450"
                     /old_locus_tag="pgaptmp_003090"
     CDS             complement(1735..1944)
                     /gene="yjbJ"
                     /locus_tag="ID725_RS15450"
                     /old_locus_tag="pgaptmp_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGV7851632.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(2060..3385)
                     /gene="dinF"
                     /locus_tag="ID725_RS15455"
                     /old_locus_tag="pgaptmp_003091"
     CDS             complement(2060..3385)
                     /gene="dinF"
                     /locus_tag="ID725_RS15455"
                     /old_locus_tag="pgaptmp_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGV7851633.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(3458..4066)
                     /gene="lexA"
                     /locus_tag="ID725_RS15460"
                     /old_locus_tag="pgaptmp_003092"
     CDS             complement(3458..4066)
                     /gene="lexA"
                     /locus_tag="ID725_RS15460"
                     /old_locus_tag="pgaptmp_003092"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGV7851634.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(4176..4544)
                     /gene="dgkA"
                     /locus_tag="ID725_RS15465"
                     /old_locus_tag="pgaptmp_003093"
     CDS             complement(4176..4544)
                     /gene="dgkA"
                     /locus_tag="ID725_RS15465"
                     /old_locus_tag="pgaptmp_003093"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGV7851635.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            4715..7138
                     /gene="plsB"
                     /locus_tag="ID725_RS15470"
                     /old_locus_tag="pgaptmp_003094"
     CDS             4715..7138
                     /gene="plsB"
                     /locus_tag="ID725_RS15470"
                     /old_locus_tag="pgaptmp_003094"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGV7851636.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(7293..8165)
                     /gene="ubiA"
                     /locus_tag="ID725_RS15475"
                     /old_locus_tag="pgaptmp_003095"
     CDS             complement(7293..8165)
                     /gene="ubiA"
                     /locus_tag="ID725_RS15475"
                     /old_locus_tag="pgaptmp_003095"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGV7851637.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(8178..8675)
                     /gene="ubiC"
                     /locus_tag="ID725_RS15480"
                     /old_locus_tag="pgaptmp_003096"
     CDS             complement(8178..8675)
                     /gene="ubiC"
                     /locus_tag="ID725_RS15480"
                     /old_locus_tag="pgaptmp_003096"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGV7851638.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(8898..10478)
                     /locus_tag="ID725_RS15485"
                     /old_locus_tag="pgaptmp_003097"
     CDS             complement(8898..10478)
                     /locus_tag="ID725_RS15485"
                     /old_locus_tag="pgaptmp_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HGV7851639.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLLRNFNGGPGLNREN
                     FIYISNIFLNIKQRNEKNHSINMFREVSISGDIVSVKFYRNEKIECACDFMMAKDAQG
                     YIDLSELDLTSCHFKGDVISKVSFISSNLQHVTFECKEIGDCNFTTAIVDNVIFKCRR
                     LHNVIFIKASGDYVDFSKNILDTVDFSQSQLTHSNFCECQIRNSNFDHCYLYASHFTR
                     AEFLTDKEISFIKSNLTAVMFDHVRISTGNFKDSVTQLMVLSIDYSDIFGNEYLDGYI
                     NNIIKMIDSLPDDPAILKSVLAVKLVMQLKILNIVNKNFIENMKKIFSHGPYIKDPII
                     RSYIHPDEDNKFDNFMRQNRFSKVNFDTQQMIDFINRFNMNKWLIDRNNNFFIQLIDQ
                     ALRSTNDTIKENAWHLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRNDNIFILFSSV
                     DDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTPETLFGSKSYDKHAL
                     FPIFTASWRANRIKNKGI"
     gene            complement(10707..11627)
                     /gene="malM"
                     /locus_tag="ID725_RS15490"
                     /old_locus_tag="pgaptmp_003098"
     CDS             complement(10707..11627)
                     /gene="malM"
                     /locus_tag="ID725_RS15490"
                     /old_locus_tag="pgaptmp_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGV7851640.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(11778..13118)
                     /gene="lamB"
                     /locus_tag="ID725_RS15495"
                     /old_locus_tag="pgaptmp_003099"
     CDS             complement(11778..13118)
                     /gene="lamB"
                     /locus_tag="ID725_RS15495"
                     /old_locus_tag="pgaptmp_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGV7851641.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(13190..14305)
                     /gene="malK"
                     /locus_tag="ID725_RS15500"
                     /old_locus_tag="pgaptmp_003100"
     CDS             complement(13190..14305)
                     /gene="malK"
                     /locus_tag="ID725_RS15500"
                     /old_locus_tag="pgaptmp_003100"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGV7851642.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            14670..15860
                     /gene="malE"
                     /locus_tag="ID725_RS15505"
                     /old_locus_tag="pgaptmp_003101"
     CDS             14670..15860
                     /gene="malE"
                     /locus_tag="ID725_RS15505"
                     /old_locus_tag="pgaptmp_003101"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGV7851643.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            16014..17558
                     /gene="malF"
                     /locus_tag="ID725_RS15510"
                     /old_locus_tag="pgaptmp_003102"
     CDS             16014..17558
                     /gene="malF"
                     /locus_tag="ID725_RS15510"
                     /old_locus_tag="pgaptmp_003102"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGV7851644.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            17573..18463
                     /gene="malG"
                     /locus_tag="ID725_RS15515"
                     /old_locus_tag="pgaptmp_003103"
     CDS             17573..18463
                     /gene="malG"
                     /locus_tag="ID725_RS15515"
                     /old_locus_tag="pgaptmp_003103"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGV7851645.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            18835..20310
                     /gene="xylE"
                     /locus_tag="ID725_RS15520"
                     /old_locus_tag="pgaptmp_003104"
     CDS             18835..20310
                     /gene="xylE"
                     /locus_tag="ID725_RS15520"
                     /old_locus_tag="pgaptmp_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGV7851646.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(20354..20764)
                     /gene="psiE"
                     /locus_tag="ID725_RS15525"
                     /old_locus_tag="pgaptmp_003105"
     CDS             complement(20354..20764)
                     /gene="psiE"
                     /locus_tag="ID725_RS15525"
                     /old_locus_tag="pgaptmp_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGV7851647.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(20890..21033)
                     /locus_tag="ID725_RS15530"
                     /old_locus_tag="pgaptmp_003106"
     CDS             complement(20890..21033)
                     /locus_tag="ID725_RS15530"
                     /old_locus_tag="pgaptmp_003106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851648.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            21034..21312
                     /locus_tag="ID725_RS15535"
                     /old_locus_tag="pgaptmp_003107"
     CDS             21034..21312
                     /locus_tag="ID725_RS15535"
                     /old_locus_tag="pgaptmp_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851649.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(21359..23455)
                     /gene="yjbH"
                     /locus_tag="ID725_RS15540"
                     /old_locus_tag="pgaptmp_003108"
     CDS             complement(21359..23455)
                     /gene="yjbH"
                     /locus_tag="ID725_RS15540"
                     /old_locus_tag="pgaptmp_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGV7851650.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(23455..24192)
                     /gene="yjbG"
                     /locus_tag="ID725_RS15545"
                     /old_locus_tag="pgaptmp_003109"
     CDS             complement(23455..24192)
                     /gene="yjbG"
                     /locus_tag="ID725_RS15545"
                     /old_locus_tag="pgaptmp_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGV7851651.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            complement(24189..24827)
                     /gene="yjbF"
                     /locus_tag="ID725_RS15550"
                     /old_locus_tag="pgaptmp_003110"
     CDS             complement(24189..24827)
                     /gene="yjbF"
                     /locus_tag="ID725_RS15550"
                     /old_locus_tag="pgaptmp_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGV7851652.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(24941..25183)
                     /gene="yjbE"
                     /locus_tag="ID725_RS15555"
                     /old_locus_tag="pgaptmp_003111"
     CDS             complement(24941..25183)
                     /gene="yjbE"
                     /locus_tag="ID725_RS15555"
                     /old_locus_tag="pgaptmp_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGV7851653.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
BASE COUNT         6276 a         6578 c         6154 g         6303 t
ORIGIN      
        1 gacttagcaa agtcaattaa tttcggtgca gcatctcgca ttttgcttaa aatatcaggg
       61 gcgagcgttc cttcattcac cagatgtgac atcttatcca gatagccatc aaaattcatt
      121 tctcccccat ctgccagacg cttcatctct cccaggtact tcttcatatc acgttgagat
      181 aattcttcaa actgagcttt caggtagtta tcattatatt cattaatatt cactcttcct
      241 gcaaaatctg agtagacact agaatatgaa agggaaatta acagcgaagc aaataatttt
      301 aagctgttgt catcatgttc ctggcaggca ttaacaaacg ttaagaaacc agaacctatt
      361 gcttgcaaat gtatattcgc taaaggttca ttatctttag attcctcata aaatagctga
      421 accgtggagg gaagaccatt cttggtttct acatcaaaag tacatcgttc tatgagtgtc
      481 gcatctttat tatcctcggg attacatata ctattaatcg cgaaatgaaa aatagccctg
      541 tcaacaactg atgcaataaa ttcacgatcg tttaattcct catcagtaca atctccaata
      601 tactcattga cacacatttt gagatcgttt atttgcttaa ttaacgtcac atttttttca
      661 cgtgcagtcc ataaattatg agtgattctt tgaataaata aatctatttt tgcaataatt
      721 atatcccgat gacgcatcgt tgaataatca atagatttac catatatact atttacttta
      781 cctttgattg catcttcatt atcgaattga tttgaattga gtgttttatt aaagtatcct
      841 gttgcaccgg gtattatctt taacatgatt catctacctt ttttgtcgta ccgtgtcctc
      901 cttataagtg tttttggaac cggtaactat caaaaatcgc tcataatttt ctgaaattcg
      961 ggtatacgat tagccacggt taaataaata gcgagttaac ttgaagcaat ttatttaaaa
     1021 atgaattatt atcgtgttga taatggtact gccaggcaac acaacatctt acctacaaaa
     1081 cagaaaagca acattacggc aacaataagg gttctcggtg tttttcctgg aacatggtaa
     1141 agtaaggaca ttcttaaccc ccactttgag gtgcccgatg gaaaagacca caacgcagga
     1201 gttattagcg caggctgaca aaatctgcgc gcagcgtaat gtgcgcctga ccccacagcg
     1261 cctggaagtg ttgcgcctga tgagtctgca ggatggcgct atcagcgctt atgatctgct
     1321 tgatttgctg cgcgaagctg aaccgcaagc caagccgcca acggtttatc gcgcgctgga
     1381 ttttctgctt gagcaaggtt ttgtgcataa ggtggaatcc accaacagtt atgtgctctg
     1441 ccatctgttc gaccagccca cccatacgtc agctatgttt atttgcgatc gctgcggcgc
     1501 ggtgaaagaa gaatgtgctg aaggcgtgga agacatcatg catacgctgg cggcaaaaat
     1561 ggggtttgcc ctgcggcata atgtgattga agcacatggg ctctgtgcgg catgcgtgga
     1621 agtggaagcg tgtcgtcatc ctgaacagtg ccagcatgat cactctgtgc aggtgaaaaa
     1681 gaaaccgcgt taagagggcg tacatccttg tacacgtcgg gcaggaggga ttaattacca
     1741 gcgatattca ttgcgggttt cccaatccac gacctctttt tctgcctgat ctttctgata
     1801 accataacgt tcctggattt taccgaccag ttgatcacgt ttaccttcaa tgatcgtcat
     1861 atcatcatcg gtcagtttgc cccattgctc tttcacttta cctttaaact gtttccagtt
     1921 accgccggct tcatctttat tcataatcaa gacctcatcg ttaggttgtg aatgagagta
     1981 cgttcacttt tcttctgaac gtgagattaa gtgtagtcag caatttggct taggattatt
     2041 tattcagaat ttttaaccgt cacgttgcgg caaaccaggt accgttgcgc cagtgacgcc
     2101 gccagatagc tgccagagaa agcccgcgca acgccagaaa gacggttaac gccaaccaca
     2161 aaccatgatt acccagccac ggcagcgtaa ggagcgtcag cgcaaaacct gcggcggcca
     2221 ccgccatact gttacgcatt tcagcagcac gcgttgcgcc tataaacatg ccgtccagca
     2281 gataacacca gacgccgacc aacggcaaaa tcacctgcca gataagatag cggtcggcca
     2341 gctgctgaat ctgggttaac gacgccagta acgcaatgat gtgttcccct gccagcaaat
     2401 aaaccaccga aaacagtaac gctacgatcc ccgactggcg gcatgctgcc cgccagacat
     2461 ccagcaactg gctaccgtcg cgcgcaccgt atgcctgacc ggaatgtgct tcaaccgcgt
     2521 aggcaaaacc gtccagcgca taggcggtaa aggtgagtag cgtcatcaga accgcgttaa
     2581 cagcgataat gtcactcccc agtcgcgcgc caagtacggt gatcgcgccg aaacagagtt
     2641 gcaacaacag cgagcgcagc atgatatcgc ggttaagtgc cagcaagcga cggaagtttc
     2701 ctcgccagga agttttcagc atttcgccgg agattccgcg tagtttgagg attttacgca
     2761 ccattagcag accaatcagc aatgttgcat attccgcaat aaccgtcgcc agcgccgcgc
     2821 cccgcacgtt catatgcagt cccatcacca gccagacatc cagcacaatg ttgaggatat
     2881 tgccgaccac taacaaaatt actggcgcac gggcatattg cacgccgagt aaccaaccaa
     2941 gtaataccag attcgccagc gacgccggtg cgcttaacca gcggatttca agaaagcgcc
     3001 gcgcctgttc caggactgct tcactaccgc caacaatatg cagcgccaga tcgataatcg
     3061 gcgtacgcag cagcgcaatt aacgccccag cccccaacgc caacagcaaa ggttgcacca
     3121 gcgcacgggc taatgcctga ggatttttgg caccataagc ctgcgcagtc agcccggtgg
     3181 tgctcatgcg taaaaacagc aacagcataa agagaaagct ggtcgccgtt gcaccaaccg
     3241 ccacgccgcc caaataaacc ggactatcaa gatgaccaat aaccgccgta tcgaccagtc
     3301 ccagcaacgg aacagtgata ttggagaaaa tcatgggtaa ggcgagatgc caaagtgctt
     3361 tatcagatga agtgaggaat gccatgcaga caagcctgat gaagagagat gaaaaacaaa
     3421 ccgcgacgcc aagcggcatc gcggtctcag agatatgtta cagccagtcg ccgttgcgaa
     3481 taacgccaac cgccagccct tcaatggtga agctctgctg acgaaggtca acgacaattg
     3541 gtttaaactc gctattttct ggcaacagtt cgactttatt gccctgtttt ttcaggcgct
     3601 taacggtaac ttcgtcatca atacgtgcga caacgacctg accgttacgt acatcctgag
     3661 ttttatgcac tgccagcaag tcgccatcca taatgccgat atctttcatc gacatcccgc
     3721 tgacgcgcag caggaaatca gcattcggct tgaacaagga aggatcgacc tgataatgac
     3781 cttcaatatg ctgttgcgcc agaagcggtt caccggcagc cacacgacct accagtggca
     3841 acccttcttc ctcttcctgc aacagacgaa tcccgcgtga tgcgccggaa acaatttcaa
     3901 ttacgccttt gcgtgccagc gccttcagat gttcttcagc cgcgtttggg gaacggaacc
     3961 ccaaacgctg cgcgatttcc gcacgcgtcg gcggcatacc tgtctggctg atgtgatcac
     4021 ggatgagatc aaacacctct tgttgcctgg ccgttaacgc tttcattccg ccccctgggt
     4081 gtatatacag ttatgctgtg agtatataca gcaaaaggcg attttggaac cataaactgc
     4141 acaataaacc agagatttat cgaattctgg aagggttatc caaaatgcga ccataacagg
     4201 atgcaccagg taatcacggc gacgataatc gcaatcagca ccgcggcgga tcccatatct
     4261 ttagcgcgtc cggaaagctc atggtattca gagccaattc ggtcaaccac tgcttcgatg
     4321 gcgctattga ggatttccac aatcatcacc agcatcacgg agctgataag cagcacgcgg
     4381 gtaatcgcgt ccacatccag ccagcaggcg atgaccaccg ccaacaatac cgctacgcct
     4441 tcctgacgga atgccgcttc gttgatccat gcagcgcgta aacctttcca ggaatagcca
     4501 gcagctttga ttattcgggt gaatccagtg gtattattgg ccattgaaag aaccttttta
     4561 cattatgagc gtcaatatca gtgtaccgtt aaatccgatg attcgggttt gcgtaacgcc
     4621 gcgcgaaaca tgagcggata ccacagaatt tcccatgact ttctgctatc cttgccgcgc
     4681 atttgcatta ttaaccagag gctttacatc gtttatgtcc ggctggccac gaatttacta
     4741 caaattactg aatttaccat taagcatcct ggtaaaaagc aagtctattc cggcagatcc
     4801 tgccccggaa ctggggctgg atacctctcg tccaattatg tacgttttac cgtacaactc
     4861 gaaagcagat ttgctgacgt tgcgcgccca gtgtctggca catgacttgc ctgacccgtt
     4921 agagccgctg gaaatcgacg gcacgctact gccgcgctat gtgttcattc acggcgggcc
     4981 gcgtgtattc acctattaca cgccgaaaga agagtctatt aagctgttcc acgactatct
     5041 cgatttgcac cgtagcaacc caaatctgga tgtgcagatg gtgccagtgt cggtgatgtt
     5101 tggtcgcgcg ccggggcgtg aaaaaggcga agtgaacccg ccgctgcgta tgcttaacgg
     5161 cgtacagaaa tttttcgctg tactgtggct cggtcgcgac agttttgtgc gtttctcgcc
     5221 gtcagtttcg ctgcgccgta tggcggatga acacggcacg gataaaacta tcgcgcagaa
     5281 actggcgcgc gtggcgcgta tgcactttgc ccgtcaacgt ctggctgccg taggaccacg
     5341 tctgcctgct cgtcaggatc tgtttaataa gctgctcgcc tcccgcgcca ttgccaaagc
     5401 ggtagaagat gaagcgcgca gcaaaaaaat ctcccatgaa aaagcgcagc agaacgcgat
     5461 tgcgctgatg gaagagattg cggcgaattt ctcttacgag atgatccgcc tgactgaccg
     5521 tattctgggc tttacctgga accgccttta ccagggcatc aacgtccata acgccgagcg
     5581 cgttcgccag ctggcccacg acggtcatga gctggtatat gtgccttgcc accgcagtca
     5641 catggactac ctgctgcttt cttacgtgct gtaccaccag gggctagtgc cgccgcatat
     5701 cgccgccggg atcaacctga atttctggcc tgccgggccg attttccgcc gtctgggtgc
     5761 gttctttatt cgccgtactt ttaaaggcaa taagctctat tccaccgttt tccgtgagta
     5821 tctcggcgaa ctgttcagcc gtggttattc cgtcgagtac ttcgtagaag gcggtcgttc
     5881 ccgtacgggg cgtttgctgg atccgaaaac cggtacgctg tcgatgacca ttcaggcgat
     5941 gctgcgtggc ggcacgcgtc cgattacgct gattccgatc tatatcggtt atgagcacgt
     6001 catggaagtg ggtacttacg ccaaagaact gcgcggcgcg acgaaagaga aagagagcct
     6061 gccgcagatg ctgcgcggtt taagcaagct gcgtaatctc ggccagggtt acgtcaattt
     6121 cggtgaacca atgccgttga tgacctacct taaccagcac gtaccagact ggcgtgaatc
     6181 tatcgatccc atcgaagcag tgcgtccggc atggttaacg ccgacggtca ataatattgc
     6241 tgccgatctg atggtacgca ttaacaacgc aggcgcggca aacgccatga acctgtgctg
     6301 tactgcgcta ctggcatcac gtcagcgctc actcacccgc gagcagttaa ccgagcaact
     6361 caactgctac ctggatctga tgcgcaacgt gccttactcc acggactcta ccgttccttc
     6421 agccagcgcc agcgagctta tcgatcacgc gctgcaaatg aacaagtttg aagttgagaa
     6481 agacactatc ggcgacatca tcattctgcc gcgcgagcaa gcggtgctga tgacctacta
     6541 tcgcaacaac attgcgcata tgttggtgct accttcgctg atggcggcaa ttgtcaccca
     6601 gcatcgccac atctcccgcg acgtattgat ggagcacgtc aatgtgcttt acccaatgct
     6661 gaaagcggag ctgttcctgc gctgggatcg cgacgagttg ccggacgtta ttgatgcgct
     6721 ggcaaatgag atgcaacgtc aggggctgat taccctgcaa gatgatgagt tgcatatcaa
     6781 cccggtgcat tctcgcacgc tacagctgct cgccgcaggc gcgcgcgaaa cgctgcaacg
     6841 ttatgccatc accttctggc tgttgagcgc caacccgtcg atcaaccgcg gtacgctgga
     6901 gaaagagagc cgcaccgtcg cgcaacgtct ctccgtgctg cacggcatca acgcgccgga
     6961 gttcttcgac aaggcggtgt tcagttctct ggtgcttacg ctgcgtgatg aagggtatat
     7021 cagcgatagc ggcgatgccg aaccggcaga aacgatgaag gtttatcagt tgctggcgga
     7081 gttgattaca tcagacgtgc gtttgacgat tgagagtgcg acgcagggcg aagggtaatc
     7141 agagagaatt gccggatgcg gcgcaaacgc cttatccggc cttccataat ctgcaaattc
     7201 aataaattgc gatctgttgt gtaggcctga gaagcgcagc gcatcaggca acccagaaga
     7261 aagccggatg atcatccggc ttttttacat catcagaaat gccagtaact cattgccagc
     7321 cctaaaaaca gtaccagacc aacatagtta ttattcataa atgctttaaa gcaggcttcg
     7381 cgctcgcggt tggcaatcag tttttgttga taaacaaaca gcgcgccagc caccagaatt
     7441 gaccagtaat atccccagcc taagccattt aactcaccga taatcgccat cagcgccagt
     7501 acgccaatct gcaaaatacc aataatcaat ttatcgtatt ggccgaacag gattgcggtg
     7561 gatttaatgc caatcttcac atcatcatca cggtcaacca tcgcatactg cgtgtcgtaa
     7621 gccaccgccc agagaatatt ggcgaggaac attaaccagc aactcaatgg caccgactca
     7681 ctcacagcag caaaagccat tggaatcgac cagccaaacg ccgcgcccag caccacttgc
     7741 ggtagatggg tataccgctt cataaacggg tacacccacg ccagcgctag cgcggcaatc
     7801 gacaacagaa tggtcatcgt attcagcgtc agcaccagta aaaacgaaat cagtaccagc
     7861 actacaaaca gcgcgcgcgc ctctttctct gttaccgcgc cgctgggaag tggtcggttc
     7921 gccgtgcgct taacatgacc atcaaacttg cggtcagcat aatcattcac cacacatccg
     7981 gcagcgcgca tcagccagac acccgcgaca aacaccgcca ggatccagag ctggggaacg
     8041 cccggtgtcg ccacccacaa cgcccataat gttggccaga gcagcagtaa cgcgccaatt
     8101 ggcttatccg tacgcattaa gcgatgaaac gccagcagct tattctgcgt cagactccac
     8161 tccatatttt tttcctctta gtacaacggt gacgccggta aaaacagttc tgttagcaac
     8221 agcggtttac cgcttaatcg caagcgggaa cgtcgccccc acagtccggc atcacggcct
     8281 atctcaataa agtcccgggt taatgtcgat gatgtgaaca gatagcgtcc taacggcgtt
     8341 ttacccaatt tttgtaacgc cagctccggc ccgcttaacg ttgacacagg aacgacggta
     8401 cgaccggcaa gccacggttc accatcggca cataacaaaa tttcacgtaa ccagtaacga
     8461 gactctttcg gcagcagcgg cagttcttcg gggatttcat tctgctcgac aaacccttcg
     8521 cggatcatcg tcacgctcac cgtttttccc tgctgttcaa aacgttttgt catggaatct
     8581 tccagcaaca gccagtcgag cagttgcggc tccagggcag ggatctcttt acaatagcgc
     8641 agcgcacgca gttgcgttaa cgcggggtgt gacatgccga actctccgtt acataacgta
     8701 aaggcattgt atcgcagaaa agtgtttacg ggagatgtaa agcaaattaa gtgatctaat
     8761 gcgcaacaaa aacgcaacaa tcgcatgaag tggtaaaacg ctgttgaacc ttacgctttg
     8821 ccgatgcccg tgctggcgag ggcaaccata ggggcatcac cgcccatgat gccaagagcg
     8881 gtgatgtttt cctttactta aataccttta ttctttatac gattagctcg ccaactcgca
     8941 gtaaaaatgg ggaaaagcgc atgtttatca taagatttgg agccgaaaag tgtttccgga
     9001 gtaagattaa caggcaacat ctcatgtctg gatttataga tatacgtgga attccaattg
     9061 gtatcttttg taggattcaa catatcatgt aagcgctggg agcttaccac cataactggc
     9121 ccatcatcga cagaggagaa caagataaag atattatcgt ttcgtgttaa ttcattcgtg
     9181 ttaaaggttc ttaaattatc ttcaatttct ataaataaag gtgaaacatc atcactacga
     9241 atccactctt tataaagatg ccaggcattt tctttgatcg tatcattcgt tgatcgtaga
     9301 gcctgatcga taagttggat aaaaaaattg ttatttcgat caatcagcca tttattcata
     9361 ttaaatctgt taataaaatc gatcatctgt tgggtatcga aattcacctt actgaatcga
     9421 ttttgacgca taaaattatc gaacttgtta tcttcatcag gatggatata actacgtata
     9481 atgggatctt ttatataagg accatggcta aatattttct tcatattctc aataaagttt
     9541 ttattaacaa tattaagaat ttttaattgc atcaccagtt ttactgccag aacggatttc
     9601 aatatcgctg gatcatctgg caacgaatca atcattttta taatgttatt gatataacca
     9661 tcgagatatt catttccaaa tatatctgag taatcaatag ataataccat tagttgtgta
     9721 acgctgtctt taaaattccc tgtcgatatt cgcacatgat caaacataac agctgttaaa
     9781 ttcgatttaa taaatgatat ttctttgtca gtaagaaatt ctgctctggt gaagtgcgaa
     9841 gcataaagat aacaatgatc gaagtttgaa tttctaatct gacattcaca aaaattacta
     9901 tgagtaagtt gactctgcga gaagtcaact gtatcaagaa tatttttgct aaaatcgaca
     9961 taatcaccac tcgctttgat aaaaattaca ttgtgtaaac gtcgacattt aaatatgaca
    10021 ttatcaacta ttgcagtagt aaaattgcaa tccccaattt ctttacattc gaatgttaca
    10081 tgttgtagat ttgatgatat gaaagacacc ttcgaaataa cgtcaccttt aaaatgacaa
    10141 cttgttaaat ccaattcaga caggtcgata tacccctgcg catctttagc catcataaaa
    10201 tcacaagcgc attctatttt ttcatttcta taaaatttta cgcttacaat atcaccactg
    10261 attgagactt cacgaaacat atttattgaa tgattttttt cgttccgttg ttttatattt
    10321 aaaaaaatat tgctgatata aataaagttt tctcgattta accctggccc cccattaaaa
    10381 ttacgtagta aatcaaaatc tactatatta tttccttgca ccacaccatg ttttatcaaa
    10441 gagttattgc cgtcaaactt tgagatatga cctaacataa aacctctgca ttacagaaat
    10501 gaaataactg gaaaaagtag cctgagcgtc cagacactac cggcgaaagg aaaagaatct
    10561 ctgataatgc attgagataa tggatattct tagtgaagaa gaagtgagtt actataaaaa
    10621 atcgctcata tccctggaat gaagcataaa aaaaggtgcg ccaggagacg caccagttgt
    10681 tgcaaaatca gcactgtggg gcgtaattac cccttgcctt ttacactgct gataaaggtg
    10741 gaacgggcag atgtcgatcc caggcgttca gcttcatcaa gcagttttaa cgccttatca
    10801 acatcacctt tcgcgacagc gtttttgatc gcggtattaa aataactttc cgtgtcgttg
    10861 agcatcggct cgcttttctt caccggcacc ggagcgggtg cagccacagc tggtgccgcc
    10921 gtgttgccta ccgtaaccgg agctggagcg gaagaaccaa ataaaggccc gaccaacacg
    10981 ctggagctgg agttcgtttt cactttcagt ttcagtaagc catcggtggt atgacgagca
    11041 accggatcgg gaatatccgg gatcgagtta ccaacgcctt tggcataggc tttagccggg
    11101 tcgagcagtt gggtcgtctg ctggagatct ttttccgtgg taaagaccag aacataaagt
    11161 ttttgctgcc ccaacgccgg tgtcaggcgc ataacgcctt ccagccgatc tgcactcatc
    11221 acgcctggtt cctggtaggt gaaataactg ctggggaaga aggctgatgg ggtcatgttc
    11281 tgatcaagaa tcagcacgtt cggcgcaaaa acgctggttt gtttgttcac ttcgctggtc
    11341 agcgtcaggg tcagttcgcc aatgtttgcc gggacgctgt acgcagcaac cggaccactg
    11401 atgccgggaa cgttcagttg ttggccgccg gtcgccagtt gggtggtctg ggttttagat
    11461 tgatcgaccg gtgtccaggt gagttgttgc agcgcggcag atggaatggc tggcgcgtcg
    11521 ctggtgtttt gcggtacgta gttaacatcg gcaaggctga ttccaggcgc gcttgccagt
    11581 aaccctgctg ataaacagag ggcgatgaga cttttattca ttttcattgt tttcacctca
    11641 aaatctggag ctcagcggta gccaggcaat agcgcgctaa acccgataat cagaggggct
    11701 ttcgcccctt tcagataatg acaacctgtt ttatgccgga tgcggcgtaa acgccttatc
    11761 cggcccaggt tttgctatta ccaccagatt tccatctggg caccgaaggt ccactcgtcg
    11821 ctgtcgccac gaccgaagct gccgccgttg aaatcagcag gaacggcttt gccgtagttc
    11881 gggttaacct tgctatcgcc gttgtagtcg taaccccatt tctcatccca cttggcgtag
    11941 gttgcgaaga cacgaatagc cgggcgtgac cagatgctgt cgccagcctg ccattgttgt
    12001 gcgagggtaa ttttgtactg attgttcttg tcgccggtgc gctgggattc gacgttgtcg
    12061 tagccgattt ccatcacggt gctcatgatt ggcgtccact tgtacatcgg gcgaataccg
    12121 acggtccacc acttggtgcc gttgtcgtta tcccagttga tatcctggta catacccacg
    12181 tacatcatgt cccagttgtc gcccatggag atcgcaccgt ggtcgaggat acgcagcatg
    12241 tgaccgttgt tgttgatatt gtaggcaaat ttttcgttat caaatgcaac gccagaaccc
    12301 tgcgacagac ctttaccctg cgaggtcatc gagtcagtag cgtactgaac aacaaacttg
    12361 ttaaagccct tcaggacact ctgagtatgt tcagcagtga ataaccagcc gtctttcgat
    12421 gcgccatcaa ccagacgata gttatcacgc aggttggcac gaccgtagtc gacacccagt
    12481 tctaatgtgc cgcccgggtt gacttccatc tgcgctaaac gcacatcgaa aacgtcgttc
    12541 gcggtttcgt tggtatagtc ataaatattg ttgctggcga aagaggaaga accacctgct
    12601 tcagaggagc gggttgctgc cagagagagt ttaccgaagc caacatcgat gttttccaga
    12661 ccggcaccag gaccagaaat atcccagtag tagaagtcga tcatatgaac gtcatgacgt
    12721 tggtagaagc gcttacctgc ccagatggtg gagcctggca gccattcgat caggttttta
    12781 ccctgcacgt ttgcttcacg gaaggccggg tcggtagctt cccagtcatt ctgttgcgcg
    12841 acggaatagg ccacgttagt gtcgaaatag aagctcttat cgccctcttt ccacacttcc
    12901 tgacccaatt ttaattcagc ataagtttca cattcgttgc caagacggta tttactttga
    12961 gcaccggtag tctggaaaca ctgttgttca ccgccgctac ctgtccagcc aataccggaa
    13021 cgtgcatagc cgtggaaatc aacagccatt gcctgagcag acattacgcc cgctgcgacg
    13081 gcaaccgcca gaggaagttt gcgcagagta atcatcattc tatctcctga gtcattgctt
    13141 ttcttttttc acatcacctg tgacaggctt tgtgtgtttt gtggggtgct taaacgcccg
    13201 gctccttatg cagtcgacga catgcagtgc catcctcacg gaacagatgg caacgctctg
    13261 gcggcaggcc gatagcgaat gtggcacctt cttctaccaa caccacgtcg ttctggcggt
    13321 acaccaggtt ttgacgaatg gaagggatct ggatatggat ttgggtttcg ttgccgagtt
    13381 gctcgacgac ctgaacttca ccctcaagga tgacgtcagc gatatcactc ggcagtagat
    13441 gttccgggcg aatacccagc gacatattgg ctccaacctg gacatcacgg ctttcaactg
    13501 gcagccagac ttgctgacga tttggcatcg gcagctccac ctgcacttga tcgattgcgg
    13561 tggcggtcac ttttaccggc aggaagttca tctttggcga accgataaat ccggcgacaa
    13621 aacggtctgc cggatagtgg tacagctcca gcggtttccc aacctgcgcc acgcgaccgg
    13681 cgtccagcac cacgattttg tcggccagcg tcatcgcttc gacctgatcg tgggtgacgt
    13741 aaatcattgt gcggcccagg cgtttatgca gacgggagat ttcgatacgc atttgcacac
    13801 gcagtgcagc atcgaggttg gagagcggtt catcgagcaa aaatacgctt ggctcggcca
    13861 ccagcgtacg gccaatcgcc acacgctgac gctgaccacc ggagagcgct ttcggtttgc
    13921 gatccagcaa atgcgccagt tgtagcactt ccgccacctg gttgacgcgt tggttaatca
    13981 cctctttttt tgcgcctgcc agtttcaggc caaatgacat gttttctgct actgacaggt
    14041 ggggatagag cgcgtaggac tgaaacacca taccaacgcc acgttctgct ggcggagtgt
    14101 cattcatccg tttctcaccg atgaacaggt cgccgctggt gatcgtctca agcccggcaa
    14161 tcatgcgcag taaagtcgat ttaccgcagc cagacggtcc gacaaacacc acgaattcac
    14221 cttcatggat atcgagattg atatctttcg ataccacgac ctcgccccag gctttcgtta
    14281 cattttgcag ctgtacgctc gccatgccct tctccctttg taacaacctg tcatcgacag
    14341 caacattcat gatgggctga ctatgcgtca tcaggagatg gcttaaatcc tccaccccct
    14401 agctttttta tgggggagga ggcgggagga tgagaacgcg gcttctgtga actaaaccga
    14461 ggtcatgtaa ggaatttcgt gatgttgctt gcaaaaatcg tggcgatttt atgtgcgcat
    14521 ctccacatta ctgccaattc tgtaacagag atcacacaaa gcaacggtgg ggcgtagggg
    14581 caaggaggat ggaaagaggt tgccgtataa agaaactaga gtccgtttag gtgttttcac
    14641 gagcacttca ccaacaagga ccatagatta tgaaaataaa aacaggtgca cgcatcctcg
    14701 cattatccgc attaacgacg atgatgtttt ccgcctcggc tctcgccaaa atcgaagaag
    14761 gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta
    14821 agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag
    14881 agaaattccc tcaggttgcg gcaactggcg atggccctga cattatcttc tgggcgcacg
    14941 accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt
    15001 tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg
    15061 cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctaccgaacc
    15121 cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga
    15181 gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg
    15241 ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata
    15301 acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga
    15361 atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga
    15421 ctatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa
    15481 cggtactgcc gaccttcaag ggtcaaccat ctaaaccgtt cgttggcgtg ctgagcgccg
    15541 gtattaacgc cgccagtccg aacaaagagc tggcgaaaga gttcctcgaa aactatctgc
    15601 tgactgacga aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga
    15661 agtcttacga ggaagagttg gcgaaagatc cacgtattgc agccaccatg gaaaacgccc
    15721 agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta
    15781 ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc
    15841 agactcgtat caccaagtaa tgctgtgaaa tgccggatgc ggcgtgaacg ccttgtccgg
    15901 cctacaaaac cgaaacgtat gtaggcctga taagacgcgt cagcgtcgca tcaggcagtt
    15961 gttgtcggat aaggcatgaa agccttatcc gtcctggaat gaggaagaac cccatggatg
    16021 tcattaaaaa gaaacattgg tggcaaagcg acgcgctgaa atggtcagtg ttaggtctgc
    16081 tcggcctgct ggtgggttac cttgttgttt taatgtacgc acaaggggaa tacctgttcg
    16141 ccattaccac gctgatattg agttcagcgg ggctgtatat tttcgccaat cgtaaagcct
    16201 acgcctggcg ctatgtttac ccgggaatgg ctggaatggg attattcgtc ctcttccctc
    16261 tggtctgcac catcgccatt gccttcacca actacagcag cactaaccag ctgacttttg
    16321 aacgtgcaca ggaagtgttg ttagatcgct cctggcaagc aggcaaaacc tataactttg
    16381 gtctttaccc ggcgggcgat gagtggcaac tggcgctcag cgacggcgaa accggcaaaa
    16441 attacctctc cgacgctttt aaatttggca gagagcaaaa actgcaactg aaagaaacga
    16501 ccgcccagcc cgaaggcgaa cgcgcaaatc tacgcgtgat tacccagaat cgtcaggcgc
    16561 tgagtgacat taccgccatt ctgccggatg gcaacaaagt gatgatgagc tccctgcgcc
    16621 agttttctgg cacgcagccg ctctacacac tcgacggtga cggcacgttg acgaataatc
    16681 agagcggcgt gaaatatcgt ccgaataacc aaattggctt ttaccagtcc attaccgccg
    16741 acggcaactg gggtgatgaa aagctaagcc ccggttacac cgtgaccacc ggctggaaaa
    16801 actttactcg cgtcttcacc gacgaaggca ttcagaaacc gttcctcgcc attttcgtct
    16861 ggaccgtggt gttctcgctg atcactgtct ttttaacggt ggcagtcggc atggttctgg
    16921 cgtgtctggt gcagtgggaa gcgttgcgcg gcaaagcggt ctatcgcgtc ctgctgattc
    16981 tgccctacgc ggtgccatcg ttcatttcaa tcttgatttt caaagggttg tttaaccaga
    17041 gcttcggtga gatcaacatg atgttgagcg cgctgtttgg cgtgaagccc gcctggttca
    17101 gcgatccgac caccgcccga acgatgctga ttatcgtcaa tacctggctg ggctatccgt
    17161 acatgatgat cctctgcatg ggcttgctga aagcgattcc ggacgatttg tatgaagcct
    17221 cagcaatgga tggcgcaggt ccgttccaga acttctttaa gattacgctg ccgctgctga
    17281 ttaaaccgct gacgccgctg atgatcgcca gcttcgcctt taactttaac aacttcgtgc
    17341 tgattcaact gttaaccaac ggcggcccgg atcgtcttgg cacgaccacg ccagccggtt
    17401 ataccgacct gcttgttaac tacacctacc gcatcgcttt tgaaggcggc gggggtcagg
    17461 acttcggtct ggcagcagca attgccacgc tgatcttcct gctggtgggt gcgctggcga
    17521 tagtgaacct gaaagccacg cgaatgaagt ttgattaagg gagataacaa aaatggcaat
    17581 ggtccaaccg aaatcgcaaa aagcacgttt gtttattact cacctgctac tgctactttt
    17641 tatcgcggcg attatgttcc cgctgctgat ggtcgtcgct atctcactgc gccaggggaa
    17701 ctttgcgacc ggcagcctga tcccggagca aatctcctgg gatcactgga aactggcatt
    17761 aggttttagc gtggaacagg ctgatggtcg cattacgcca ccgccattcc cggtgctgct
    17821 gtggctgtgg aactcggtaa aggtcgccgg gatttccgcg attggcattg tggcgctctc
    17881 caccacctgc gcctacgctt tcgcccgtat gcgctttcca ggcaaagcga cgctgctgaa
    17941 aggaatgctg attttccaga tgttcccggc agtactttca ctggtcgcgt tgtatgcgtt
    18001 gtttgatcgt ctgggtgagt acattccatt cattggcctg aatactcacg gcggcgtaat
    18061 tttcgcgtat ctgggtggga ttgcgctgca cgtctggacc atcaaaggct atttcgaaac
    18121 catcgacagt tcgctggaag aagcggctgc gctggatggt gcgacaccgt ggcaggcctt
    18181 ccgccttgtc ctgttgccgc tgtcagtacc gattctggcg gtggtattca tcctgtcgtt
    18241 tatcgctgcc attactgaag ttccggtcgc gtcgctgtta ctgcgtgacg taaacagcta
    18301 caccctggcc gtggggatgc agcaatacct caatccgcaa aactacctgt ggggtgactt
    18361 tgccgccgct gccgtgatgt ctgcattacc gatcaccatc gtcttcttgc tggctcaacg
    18421 ctggctggtc aacggcctga cggcaggtgg tgtgaaaggt taaagatgtt gttctgccaa
    18481 tgttatgccg ctgcaccctc aacttacgtt atcccaactt gtgactgtta ttcggcgctc
    18541 cacggagcgc ctttttttct ttcgtctgca atctgaatcg ttcgccggtt aatatttcca
    18601 tcatagagct tattattttt acgttatttg ttttcccact tacgataatt ctctttcgtg
    18661 ctctgagtca cggcaatagt attgttttta tcaattttgg ataattatca caattaagat
    18721 cacagaaaag acattacgta aacgcattgt aaaaaatgat aattgcctta actgcctgac
    18781 aattccaaca tcaatgcact gataaaagat cagaatggtc taaggcaggt ctgaatgaat
    18841 acccagtata attccagtta tatattttcg attaccttag tcgctacatt aggtggttta
    18901 ttatttggct acgacaccgc cgttatttcc ggtactgttg agtcactcaa taccgtcttt
    18961 gttgctccac aaaacttaag tgaatccgct gccaactccc tgttagggtt ttgcgtggcc
    19021 agcgctctga ttggttgcat catcggcggt gccctcggtg gttattgcag taaccgcttc
    19081 ggtcgtcgtg attcacttaa gattgctgct gtcctgtttt ttatttctgg tgtaggttct
    19141 gcctggccag aacttggttt tacctctata aacccggaca acacagtgcc tgtttatctg
    19201 gcaggttatg tcccggaatt tgttatttat cgtattattg gcggtattgg cgttggttta
    19261 gcctcaatgc tttcaccaat gtatattgcg gaacttgctc ctgctcatat tcgcgggaaa
    19321 ctggtttcat ttaaccagtt tgcgattatt ttcgggcagc ttttagttta ctgcgtaaac
    19381 tattttattg cgcgttccgg tgatgccagc tggctgaata ctgacggctg gcgttatatg
    19441 tttgcctcgg aatgtatccc tgcactgctg ttcttaatgc tgctgtatac cgtgccagaa
    19501 agtcctcgct ggctgatgtc acgcggcaag caagaacagg cggaaggtat cctgcgcaaa
    19561 attatgggca acacgcttgc aactcaggca gtacaggaaa ttaaacactc cctggatcat
    19621 ggccgcaaaa ccggtggtcg tctgctgatg tttggcgtgg gcgtgattgt catcggcgtt
    19681 atgctctcca tcttccagca atttgtcggc atcaatgtgg tgctgtacta cgcgccggaa
    19741 gtgttcaaaa cgctgggggc cagcacggat atcgcgctgt tgcagaccat tattgtcgga
    19801 gttatcaacc tcaccttcac cgttctggca attatgacgg tggataaatt tggtcgtaag
    19861 ccactgcaaa ttatcggcgc actcggaatg gcaatcggta tgtttagcct cggtaccgcg
    19921 ttttacactc aggcaccggg tattgtggcg ctactgtcga tgctgttcta tgttgccgcc
    19981 tttgccatgt cctggggtcc ggtatgctgg gtactgctgt cggaaatctt cccgaatgct
    20041 attcgaggta aagcgctggc aatcgcggtg gcggcccagt ggctggcgaa ctacttcgtc
    20101 tcctggacct tcccgatgat ggacaaaaac tcctggctgg tggcccattt ccacaacggt
    20161 ttctcctact ggatttacgg ttgtatgggc gttctggcag cactgtttat gtggaaattt
    20221 gtcccggaaa ccaaaggtaa aacccttgag gagctggaag ccctctggga accggaaacg
    20281 aagaaaacac aacaaactgc tacgctgtaa tcttcctgtc cagcacgccg cgccagttcg
    20341 gcgtgctgtc tttttactcc cgcttcagcc gtttcgaatt acacagccac agggtgacca
    20401 ccagtaacag gatcgcagcc gagtagatca acacatccag tggcgattta tgatcgacga
    20461 tgatcaagcg cacaatggcg gtgatcccaa tatagacaaa gtaacgtagc gggaagtgaa
    20521 aaccggactg aaagtacttc acaatcagcg cgataaattc gaaatagaga aagtaaacca
    20581 ccagtccttc taccagctca tatttgctgg cttgttctgg cgcgaacagc acatcagcca
    20641 gatgcaccgt ttctttgccg aggaagacaa ccaaaataag gccgaggcac agcaggccaa
    20701 gattgagtac ggtctgcaaa atggtggaga taaactccac gcgcggacga gagagtgacg
    20761 tcatagcagt acctccttca cagtggcgag cttcctgtat aacgcaaaaa tgtgacggag
    20821 atctatattt tttgtgtatg ttttgttcaa cctggatttg gagcgcgcct ccaccaaagc
    20881 cggatatacc tacccgtagg ccggataagc cgcgcctggc ggcgcatccg gcaagacaaa
    20941 ccgcaccaaa gccggtaatt caaaaatcac ccattcaggt gattcccagc caccagcaat
    21001 gcgcgaaaaa tcgacaatgt tcctctggaa caaatgaaac gtaacctgat aaaggtggta
    21061 aaaatgaagc cttattttgc tgctttgatg ttatcagtct ctgctcttcc tgcttatgct
    21121 ggtccgctcg gtactgctga taaagccgac ctaccgcaaa gcaatgtctc cagcccaatg
    21181 atggcccagt cgctcaggca accagacctg caaccgatat cgacggacag gaagacagaa
    21241 tgttttcgcc tttacacacc agatcgcaaa ccgggggtga actgcgttcc tgatggctca
    21301 acaggccatt aagaataaag gtgccagtga ttgcgaaaac tggcaccttt cccatgactt
    21361 agcggaaatt gacgctacgg tcgctggtca tgtcatacaa ctggaactta cgcccaagtt
    21421 gctgaccacc gtcacgcgtc agcggcgtcc agccaatcgc cgcacggctg cgtgtcgggc
    21481 cagacgagaa gagatccaac ggtacagaga catacacgcc tttggtgaag tccccttcgc
    21541 cgtactcctc tttcgaaacg ttggtgatcg tggcatagcc gcccaccacc acgccgctgt
    21601 caaagcgttt agcgatctcc agcgtgccgc ctttatcccc tgccagatac tgcccgacgc
    21661 tggctttaac taacacatcc tgagcgaaag atggcgtcca gtaggcggtc agatgtccgg
    21721 ttttcacgct gtagtcggtg aatttcatca tatcctttgc actacgccag tcgcgctgtt
    21781 taacgtagtt ggcatccaga ccaaacgccc agttgctgtc cagcgggcga tacaacactt
    21841 ctgcccccgc accgccaaac atggtttcga gataacctcc gtagacctga ccgtagaagc
    21901 cgttgcccag atgctggaag tagttggctt gcaggttatt cacatagaca tcgttctgca
    21961 catactcgcg cacatgggta cgcacgcgcg gtaagtgcga gtcctgcggc ggattggtgt
    22021 agttaaactt gtcgtagttg ttggcaagat ttgcaaacag gctgccggtg gtcagcagat
    22081 ggtccgtcag ccacaaatct gccgttccca tcacgcccag ctgatacatg taaaagtttt
    22141 ccgggccacc gaccgactgg ttcagcaccg gatcgatatg gaaatcaaag cgcgatttgt
    22201 cgatatacca gccctgctcg gtggacttcg gaaccactgg ctcgacgcgt ttttgcgcca
    22261 gcttcgtttc gtggcccaac ggctctccgg cgagatgatt tttcaggctg gcgacatccg
    22321 tttccgtcgt cacctgcggc atgttaaggc gattttccgt aacgcggatc gtcttgatcc
    22381 catccggcag atcattcatc acgatccgat tagcacgaac gatcccttcg cgcgaatcac
    22441 gatatttcac ctgctcgccg gtaacataca gcgtatcgcc tttcgcctgg atctgcggat
    22501 cggcaagtcc ggcattgtat ttcaacagcg ttaactgatt cgccaccacc gaatgctgca
    22561 aaatggcatc ctgcggctgc ggttgatatt gcgggcgggc gttatcgttg taagacgggc
    22621 gcagatcgtt aaagttggtg cgcagcgtga cgccaaacat aaaagtgttg gcacgttcat
    22681 agctaaggtt aacgtcggcc caatcggtaa cgcgataaat cgccccgacg ttaaacttac
    22741 ttttttgctc cagcttaccg gcaaaatcct gctgataatt attgccttca tactccagtt
    22801 tcaggcgcag cggttgccag ggcgtctggt attccacgcc gccaaacagt gaggcaggac
    22861 cgtggaacat ctggctaccg tcgatagatc ccgcctgttt gtagctgtta tcgcgatagc
    22921 aatatttatc actggctgaa cagagcggat ttttcacatt accgcttgtg cccaaatatc
    22981 cccagcccag gccaagcgta aaatcgaacg gcccccaggc tttgctggca acaagatatt
    23041 ccgcatcaaa cagccccgta ccgccgatat cccgcgcacc aaccgccact tgcggcagcc
    23101 agtaactctc ttcccacaaa cgcagtttga gatcgaaggc tttatcttta tacgtttgat
    23161 cgccagagaa cgcttcgacg ctgctgtact gccgggtgcg cacgtcggtg tagcgcagcg
    23221 ttgtttccag ccacgggaag agttgcactg aagctgagta ataacggtac tgatcgttat
    23281 cgcgatagtt cagactcaac tccccttccc gcgccatgcg agccgtgggc gtttgtaata
    23341 atcctacgcc accgaaatcc gactgcgacg gaccaatggg cgcaggatat gtttcgccgt
    23401 agcaagctgt gctaatgccc agcgccagta agctaagcag atgtcttttt ttcattattg
    23461 aggtatccgc tgcgtcagag tctgaagaat gtcggcgtta agggcatcag gcatctcact
    23521 ccagacggag tccgcgaggc caacataaat aatgctgccg ggcatcggct ctacgtgacg
    23581 cttgttccag taagccaccg gcgctttttg tgtgcgtccg tccgggtaaa ccacccacgc
    23641 gtagctacga tccgcaccgc taagcagact ttgcccggag agatagctcg ccacgtcgcg
    23701 accgggagtg aatggctgat tgccaggacg gctgataagc ccgaacaacg tgaccgtgga
    23761 cggtggtggt ccgacccaca gcgtgtagtt gccctgcaac ggcgggttag cgcgttcagc
    23821 aacgcggacg atatcgggat caagattgat tttttgcctg cccgtcacct tcaacgcctg
    23881 aatttgctgg cgtaaggcgt taatcgcagc ggcatcgtcg gcgctggaat ctgcgccctg
    23941 ttctgccagc cgtgtcagca acgcctgctg ctgacgcaat gctgccgccg ttgccagctc
    24001 ttcgctaatc accgcaccag gccaccagct gtttgccagc cgcggttggc ctaccagatc
    24061 gattaaatgc tctgcgcccg ttagcgtttt ggcctcactg ctgccattgc tgaacacctt
    24121 gacggttcct gccgcgaaga ccgatgacgc tcccacactc aaaagcaacg cgacaatagt
    24181 ttgtttaatc attgttttgc cgccttgatg agagtggttt tcaccgggaa ataatccgcg
    24241 ccgagatact gttccgactg gcgaatttgc cctccgctgt cgatccaata gcggttatgc
    24301 cagcgcgcct ggtcggtgga gacttcttcg tccagcacgc gaaccgcggt ttcatcgctg
    24361 ccgaccttaa cggtaccggt gccatcccat ttgaagactg agcgtgcggt ggcgtagcgt
    24421 acctgctggt actcggtcca gcccatcgtg cgcatccagg ttgcgccatc aatgatttgt
    24481 gcgggtttaa tcagcgggtc ggcggcaagg ttattcactt caatcaggtt gtcgccgcca
    24541 agcaaagtct tcaccagacg accatgttgt gtgacgagag tggcctgatc ctgagtgacc
    24601 catttttgtt gtccgtcttc agcgaaggcc agcaccacaa acaactgcgg cccaccatta
    24661 agctgcatgt actggctggc gtagggcata ttttgaatat catcgtccgt cagctgtacg
    24721 cctggcgtgc cgaacagact gtcccacagt gaattgccca gctctttagt cgtggctgaa
    24781 caggcctgta atagcaggca gataagaatg aatgcaggtc gcttcacgac tcttctccag
    24841 aggggcagaa taaccacacc ggagtgtggt tatggtaaat cccttcatac ttctagccac
    24901 agatgcaatg actcgaatta ttttggggat acatacttta ttactgggta ctggtggtcg
    24961 tggttgtggt ggtcccggta ttagaaccat caccgccacc ggttgccgcg agcgcgacac
    25021 ccaccgccga gcttacggtg ctgacgctgg tcgcggagga actccccgcc gaaaccgacg
    25081 ttgctgccga ccctgccgct tcgcccacct gtacaggtgc agcccacgca gaagtcgccg
    25141 caagcgcaga tatggcaaaa atgccataca gaactttttt cataacaatt tcccttcatt
    25201 gaatgaatgg agatttaccc aaaaacattt cgggcgggat cgagtataca caactaaagc
    25261 atgcgaatta cggcgcgggg aattagagat ggggttttag gataattgga a
//
LOCUS       DABSYT020000064        24814 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.064, whole genome shotgun sequence.
ACCESSION   DABSYT020000064 DABSYT020000000
VERSION     DABSYT020000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.064"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            81..821
                     /gene="yiaT"
                     /locus_tag="ID725_RS15560"
                     /old_locus_tag="pgaptmp_003112"
     CDS             81..821
                     /gene="yiaT"
                     /locus_tag="ID725_RS15560"
                     /old_locus_tag="pgaptmp_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGV7851654.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(951..2921)
                     /locus_tag="ID725_RS15565"
                     /old_locus_tag="pgaptmp_003113"
     CDS             complement(951..2921)
                     /locus_tag="ID725_RS15565"
                     /old_locus_tag="pgaptmp_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HGV7851655.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2932..4332)
                     /locus_tag="ID725_RS15570"
                     /old_locus_tag="pgaptmp_003114"
     CDS             complement(2932..4332)
                     /locus_tag="ID725_RS15570"
                     /old_locus_tag="pgaptmp_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7851656.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4558..5373
                     /locus_tag="ID725_RS15575"
                     /old_locus_tag="pgaptmp_003115"
     CDS             4558..5373
                     /locus_tag="ID725_RS15575"
                     /old_locus_tag="pgaptmp_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7851657.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            5375..5515
                     /locus_tag="ID725_RS15580"
                     /old_locus_tag="pgaptmp_003116"
                     /pseudo
     CDS             5375..5515
                     /locus_tag="ID725_RS15580"
                     /old_locus_tag="pgaptmp_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            5617..6090
                     /gene="ysaA"
                     /locus_tag="ID725_RS15585"
                     /old_locus_tag="pgaptmp_003117"
     CDS             5617..6090
                     /gene="ysaA"
                     /locus_tag="ID725_RS15585"
                     /old_locus_tag="pgaptmp_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGV7851658.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(6242..7495)
                     /gene="avtA"
                     /locus_tag="ID725_RS15590"
                     /old_locus_tag="pgaptmp_003118"
     CDS             complement(6242..7495)
                     /gene="avtA"
                     /locus_tag="ID725_RS15590"
                     /old_locus_tag="pgaptmp_003118"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGV7851659.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7673..9703)
                     /gene="malS"
                     /locus_tag="ID725_RS15595"
                     /old_locus_tag="pgaptmp_003119"
     CDS             complement(7673..9703)
                     /gene="malS"
                     /locus_tag="ID725_RS15595"
                     /old_locus_tag="pgaptmp_003119"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGV7851660.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            10023..10847
                     /gene="bax"
                     /locus_tag="ID725_RS15600"
                     /old_locus_tag="pgaptmp_003120"
     CDS             10023..10847
                     /gene="bax"
                     /locus_tag="ID725_RS15600"
                     /old_locus_tag="pgaptmp_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGV7851661.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(10955..12133)
                     /gene="xylR"
                     /locus_tag="ID725_RS15605"
                     /old_locus_tag="pgaptmp_003121"
     CDS             complement(10955..12133)
                     /gene="xylR"
                     /locus_tag="ID725_RS15605"
                     /old_locus_tag="pgaptmp_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGV7851662.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(12211..13392)
                     /gene="xylH"
                     /locus_tag="ID725_RS15610"
                     /old_locus_tag="pgaptmp_003122"
     CDS             complement(12211..13392)
                     /gene="xylH"
                     /locus_tag="ID725_RS15610"
                     /old_locus_tag="pgaptmp_003122"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGV7851663.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(13370..14911)
                     /gene="xylG"
                     /locus_tag="ID725_RS15615"
                     /old_locus_tag="pgaptmp_003123"
     CDS             complement(13370..14911)
                     /gene="xylG"
                     /locus_tag="ID725_RS15615"
                     /old_locus_tag="pgaptmp_003123"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGV7851664.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(14989..15981)
                     /gene="xylF"
                     /locus_tag="ID725_RS15620"
                     /old_locus_tag="pgaptmp_003124"
     CDS             complement(14989..15981)
                     /gene="xylF"
                     /locus_tag="ID725_RS15620"
                     /old_locus_tag="pgaptmp_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGV7851665.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            16347..17669
                     /gene="xylA"
                     /locus_tag="ID725_RS15625"
                     /old_locus_tag="pgaptmp_003125"
     CDS             16347..17669
                     /gene="xylA"
                     /locus_tag="ID725_RS15625"
                     /old_locus_tag="pgaptmp_003125"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGV7851666.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            17741..19195
                     /gene="xylB"
                     /locus_tag="ID725_RS15630"
                     /old_locus_tag="pgaptmp_003126"
     CDS             17741..19195
                     /gene="xylB"
                     /locus_tag="ID725_RS15630"
                     /old_locus_tag="pgaptmp_003126"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGV7851667.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            19364..19705
                     /gene="yiaB"
                     /locus_tag="ID725_RS15635"
                     /old_locus_tag="pgaptmp_003127"
     CDS             19364..19705
                     /gene="yiaB"
                     /locus_tag="ID725_RS15635"
                     /old_locus_tag="pgaptmp_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGV7851668.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            19751..20188
                     /gene="yiaA"
                     /locus_tag="ID725_RS15640"
                     /old_locus_tag="pgaptmp_003128"
     CDS             19751..20188
                     /gene="yiaA"
                     /locus_tag="ID725_RS15640"
                     /old_locus_tag="pgaptmp_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGV7851669.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(20230..21225)
                     /gene="wecH"
                     /locus_tag="ID725_RS15645"
                     /old_locus_tag="pgaptmp_003129"
     CDS             complement(20230..21225)
                     /gene="wecH"
                     /locus_tag="ID725_RS15645"
                     /old_locus_tag="pgaptmp_003129"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGV7851670.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            21400..21699
                     /gene="ysaB"
                     /locus_tag="ID725_RS15650"
                     /old_locus_tag="pgaptmp_003130"
     CDS             21400..21699
                     /gene="ysaB"
                     /locus_tag="ID725_RS15650"
                     /old_locus_tag="pgaptmp_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGV7851671.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            21794..22705
                     /gene="glyQ"
                     /locus_tag="ID725_RS15655"
                     /old_locus_tag="pgaptmp_003131"
     CDS             21794..22705
                     /gene="glyQ"
                     /locus_tag="ID725_RS15655"
                     /old_locus_tag="pgaptmp_003131"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGV7851672.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            22715..24784
                     /gene="glyS"
                     /locus_tag="ID725_RS15660"
                     /old_locus_tag="pgaptmp_003132"
     CDS             22715..24784
                     /gene="glyS"
                     /locus_tag="ID725_RS15660"
                     /old_locus_tag="pgaptmp_003132"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGV7851673.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         6128 a         6112 c         6416 g         6158 t
ORIGIN      
        1 atatgaatct tgcaccggag aaattgtaag cattactatg ccgactcttt tttatcttat
       61 tttaaaatgg aataccggac atgttaatta accgcaatat tgtggcgtta tttgcgttgc
      121 cttttatggc aagcgcaact gcttctgaat tatccattgg tgctggtgcg gcttataatg
      181 aatcgcctta tcgcggttat aatgaaaata caaaggcaat tccgctgatt agttatgaag
      241 gtgattcttt ttatgttcgt cagaccacgt taggttttat tctgtcgcaa agtgaaaaaa
      301 atgaacttag cctgaccgca tcctggatgc cgctggaatt tgaccctgcc gataatgacg
      361 attatgccat gcaacagctt gataagcgtg atagtacggc tatggcgggg gttgcctggt
      421 atcaccacga gcgttgggga accgtgaaag cctctgcagc tgcggacgtt ctggataaca
      481 gcaacggctg ggtgggggag ctatcggtat tccacaaaat gcagataggt cgtctgtcgc
      541 tgacacctgc gctgggcgtt ctctattatg acgagaattt cagtgactat tactatggca
      601 tttcagagag tgagtcccgt cgtagcggtc tggcaagtta ttccgcgcag gatgcctggg
      661 tgccctatgt cagcctgacg gcaaaatacc cgataggaga gcacgtcgta ttgatggcga
      721 gcgcaggata cagcgagctg ccggaagaga ttaccgacag cccgatgatt gatcgtaatg
      781 agagttttac ctttgtcacc ggcgtgagct ggcgttttta attcaccggt ggatgtcggt
      841 gcggctggcc tacgctctcg caccgacttg tatgcctgat aagacgtgtt aagcgtcgca
      901 tcaggcaatg tgctccttac gttggatgcg gcgcgagtgc cttatccaac ctacggatgg
      961 caatgctttt cctcattcac ccagatccgc atttcgcctt caccccggtt ggcccagcta
     1021 aaccacggga taaaggtcag agtttgcgtc tggcgtttgg ctggcgcgct gtcgtaatgc
     1081 cacagcggtt gctgctctgg attgctctgt tcataccggt aacccggtgc ctggattaag
     1141 atcttatggc taaacaatcc cttgccttca aatgtcgtaa atggcgcatc ggtgggcagc
     1201 cacagattat gcagtgactc gccgttgtcg gccttttcca ggcaatacac cagcgggccg
     1261 cgctgaatcg ccacttttcc ggcgacgtga cgcaccagcg ggttaccgta aacgcggcgt
     1321 accggcatcg gcaaagtcag attcagcgta tcgccctcct gccattcgcg ggtaatgtgc
     1381 aaataccctt tacgaatatc ctgctcgacc tcttccccat tcaatatgat ctgcggctgt
     1441 gtgcaccagt ccggcagacg taaagccagc gtatgacgta ccggctgggg cgattcaacc
     1501 gcaatcgtca cctgctcctg ccacggatag ttcccgctaa cccgcaggcg cagcgtgcca
     1561 ttttctaccg gcacttccat gctgtttcct gcgtagatgt tgatatacaa cgcatcttca
     1621 cgcggcgtgt agagataatg accaatcgag gtcagcacgc gggcgatatt tggcggacaa
     1681 caagcgcagc caaaccaacg ctggcggatc ggtttaacgt gatcgtagat atggttgaat
     1741 ttcagcgatt ttggatgtac ttccagtgga ttcacataga agaaatgttt gccatccaat
     1801 gccatgccgc cgagcacagt gttgtacagt gcgcgctcca tcacatcggc atattgactg
     1861 tcgccttcca tttccagcat tcgtcgggcg aacatcatca ggccgatgga agcacaactt
     1921 tcggcgtaaa ccgtgtcatt cggcagatcg taatcgctgc tgaacgcttc gccgctgctt
     1981 tgtgagccga tgccgccggt aatatataac tgacgctggg ccatattgtt ccacagccgc
     2041 aggcagtcct gacgcttgct gtcatcgtga cttaaacgcg cgagatgcgc gacgccggtc
     2101 atcagataga caaaacgtac cgcgtgaccg atggcggttt gctgttgcgc aaggggcaaa
     2161 tgtgcctggc tgtaggcttt gtctttcacc atccacgccg ggccgtaggt gtgccagtgc
     2221 gatgtctgcc cgcgcttttc atattcctgg tcgtaatagt gcggttgcgc accacgctgt
     2281 tcgacaaaat agttcgtcag cgccaggtag cgcggctctt cagtcacttc atacaggcgc
     2341 attagtgcca gttcaatttc cgggtgacca ggataaccgt gtaacttact ttcatctgga
     2401 ccaaatacgc tgtcgatatg atcggccagg cggcaaacga cccccagcaa gcgccgtttt
     2461 cccgtggcct ggaagaaggc gactccggct tcaatcagat gacctgcgca gtaaagttca
     2521 tgacactccg ccagattgct ccagcgttct tcgggtgctt ttaccgtaaa gtaagtattg
     2581 agatagccgt cttcgcattg ggcggaggcg atcagttcga ttacctcgtc ggcggttttt
     2641 tccagttcgg cgtccggctt ctggcacagc gaccaggcta ccgcttccag ccatttggcg
     2701 acgtcgctgt cctgaaacac catcccgtaa aattcaccct cctgaagtcc ggcggcaatg
     2761 cgaaagtttt caatcgcatg gctgggttcc gcttctggga tacgatcgtt caaggcatcc
     2821 cactgataag gaatcaccac gtcgcggacc agttgttggt actgaccgag gaacggatcg
     2881 ctgaccgtca gtttatgcag atcgacttcc gaaatgttca tcttgtgctc cttaaggctg
     2941 tacatgacgc tgacgcaggt cggtagctat gcgtgacatc ataggattat tgagtttgca
     3001 aaagcggatc gcccctgcca gcagcaggtg gaatagcgca ggcagtaatg tttccatcgc
     3061 cgcgatcccg ccaagcgaac tggcggtctg gttttccacg ccaggctggt aggcgacaaa
     3121 aataaacagc aggctgatga tcccggcgct ggaggcccag gccagcttga tgaaaaacag
     3181 attgaaggcg aagttcatgc cggaagaacg tacgtgggtt ttccactcgc cgtagtcatc
     3241 ggcaaaggcc atcaatgaga agtgcagtgg caatgtgaag ccaagaatca cgccattacc
     3301 gaggatcacc gccagccaca gcgtttgata agccgggccg gagggcagga accacatcaa
     3361 caccgccagt gcagcgagga gcaggttggt gtagtaataa attttgacgg tatcgaaacg
     3421 ccgcgttaac gggctgacaa tcaccgagcc gataatggag gcgaaggtga ccatggtgaa
     3481 gaacagcgac gtgtagcccg tgctgccttg taagacgtag gtaatgaagt acatataccc
     3541 accgccgcga atgttaaaga cgttaatcag caggaaagac atgaccagca tcagcagcag
     3601 ttggtcgttg ttccgcagcc cggcaagatg ttcgcgcagg gtaaatttcc ccattgtgga
     3661 gagcggcacc cgttcacgaa cccagaagaa acagcacagg aacatcacca cggcaatggc
     3721 gcacaatacc ccgacgccca gttgatagcc gcgtgcagcg ttgccctgac cgaagagagc
     3781 taccatccac ggtaagccta ccgaaaccag aaatcccgct acgccgcaga gaacgaatcg
     3841 ccaggactgg caggagatca cttcattgtg gcgggtggtc atggtgttga tccgcgcgca
     3901 gtacggtacg ttgatggcgg tataaccgac tgaaaggagc aggtaagtac cgaacgccca
     3961 ggcgattttt actcccatgc tggcatctgg cacggtgaac gtcaggatgc cgataatgcc
     4021 gatgggaatg gcaatccata gctgccacgg acgaaagcgc ccccatcggc tttgcgtccg
     4081 gtcggcaatg acgcccatca ccgggtcgga gatggcatca aacacgcgca gtgcaataaa
     4141 cagcgtgcca accagcgccg gagttaatcc aaaaacgtcg gtgtagaaaa aggtgagaaa
     4201 attcatgatc aggcaagtta ttacggtgcc gcctgcgtca cccaggccgt agcctatttt
     4261 ttcccgtaca gataacgcat cgtcaatttt acctttagcg atatcagcgg tagtaatcgg
     4321 tgtagaggtc atagatattc ctcgtgaata gggagcaatt tttagtttta ttgaaatgac
     4381 ctgattaatt ctgccgtgaa cgggaatatc aacaaggcga gaaaagagta aaaaaaggtg
     4441 acgattccga cctgaattta aaagtgtgaa atggatcagg tgaaattaat atttttgatt
     4501 aataatcaaa aggtaatgtg tttaatcatt aatgaataag cgggaaatta catatcaatg
     4561 ctcgaattat ccatgacgtt accgattaaa gtgcaaaatg gtggattatt tatttcccgt
     4621 ggcgtaggga ggcattctgc aaggcggctg gagtcatggg aaattatctt tgtcgaaaaa
     4681 ggtcgattaa cgattcagga agaagaacgg atatttctgg tcgatgcggg agagagttta
     4741 ttactctggc cgaaccgaca acatgttggg gtggaggatt ttcccgccga tctcaaattt
     4801 tactggctgc attttgaggt taaagcgccg gacaacgacc ctcgctggct tactcatctt
     4861 agcgtgccgc aacaaacgaa ggttgtcgat ccgcaggcgc tggtggccct gtttcgccag
     4921 ttcttaagcg aacaggaaaa gcatcaacgc actccggcgc tggagtttat tgtgctgctc
     4981 attttgcagc agctcacccg tgacgcgcgt cttgatgaac aggccgatgg cgcaggcacc
     5041 acccttgcct ggaaagcgca gaaactgatc cgcacgcaat atcatcgccc gctttctacg
     5101 gcgcttctgg cccgcgagct gtactgcaat gccgattatc ttgggcgcgt ctaccggcgg
     5161 gtgtttcatc tgacgttaac agaagcaatc catcgccagc gggtgcgcga agccgaaaag
     5221 ctgttaatta gcgaagcgcg atcgctcacg gaagtggcgg cgttgtgcgg gtttaatgac
     5281 gttggctatt tccggcagat ttttcgcaaa cacaccggtt taacgccagc agcgtggaag
     5341 cggcgttaca gcaaggaaca tatcaattcg tagtgccggg gcgatttcgc tttcgacatc
     5401 acgtctgaaa caggtggggg agaaaaatct cctgaaacca ctgcgtgaaa ccgcgcaggc
     5461 tatttctaat gaactgggat ttactgtcag ggacgaccag ggcgcgatca cataacgctt
     5521 tggacaagtg ccaaaacttt aacatttcct tcgttggatc aaagcagtag ggacgcgctc
     5581 tctggcactc tgctgtttta gtgcaaagga gtgatcatga accggtttat tattgcggat
     5641 gcgacgaaat gtatcggttg ccgtacctgt gaagtggctt gcgcagtgtc gcatcaggag
     5701 aatcaggatt gcgctgcgtt gtcaccagac gagtttattt cccgtattcg tgtcattaaa
     5761 gaccacagct ggaccacggc agtagcctgt catcagtgtg aagatgcacc gtgcgcgaat
     5821 gtctgccctg ttgacgcgat aagccgcgaa catgggcata ttttcgttga acaaacacgt
     5881 tgcattggct gtaaaagctg tatgctggct tgcccgtttg gtgcgatgga ggtcgtttct
     5941 tcgcgcaaaa aggcgagggc gattaagtgc gatctgtgct ggcatcggga gacgggaccg
     6001 gcctgtgttg aagcctgccc gacaaaggcg ttgcagtgca tggatgtaga gaaagtgcag
     6061 cggcatcggc tacggcagca gcctgtttga aacgtttttc cggcgtttct caacgccgga
     6121 atagtgcgct ttgtttatgc cagatgcggc gtaaacgcct tatccggccc acaaattctt
     6181 gcaaattaaa tatattgcag aaatcatgta ggcctgataa gcgtagcgca tcaggcaatt
     6241 tttagtgact ttcagcccag gctctttcta tctcttccgc cagaatcttc acccccgcct
     6301 caattttctc cggctctggt acgtagttca tgcgcataca ttgatgcgta tgcggccacg
     6361 gtttgtccag ccctgggaag aagttgtgcc ccggcaccat cagcacgccg cgtgctttca
     6421 ggcgctgata gagctgctcg gtcgtaatgg gcaaatcctt aaaccaaagc cagaggaaaa
     6481 tggctccttc tggtttatga attaggcagc gatcttccgg taaatagcgg cgaatgatgg
     6541 cgatagtttc ctgaacacgc tggtagtaaa acggtttgat gactgtttca gacaggcgca
     6601 gcagatcgtt acgcttaatc atttcacaca tcatcgccgg accaataccg ccaggtgcca
     6661 ggctgataat gccgttcata ttggtgatgg cggtgatgat tttttcattg gcgatgataa
     6721 tgccgcagcg ggagccaggc agccccagct tggaaagact catgcacagc acgatattcg
     6781 gattccatag cgggcgcgct tcactgaaga tgatacccgg gaacgggacg ccataagcgt
     6841 tatcaatcac cagcggaatg ccgtgttgat tcgccagcgc gtcaagcttc agcaactctt
     6901 cgtcagtaat cacattgcct gttggattcg tcggccggga gacgcaaatc atcccggttt
     6961 cttcgccaat atgcagatgc tcaaaatcga cgtggtattt aaactggcct tccggcagca
     7021 gttcaatatt cggacgcgca gagacaaaca gatcttcttc cagtccggcg tcagcgtagc
     7081 caatgtattc cggtgccagc gggaacagca cttttttgac ccgaccatcg gcacggcgtc
     7141 cggcaaacag attaaataag tagaaaaacg cgctctggct gccgtttgtt agtgcaatat
     7201 tctgtgcttc gatatcccaa cccaacttct cgcgcagcat tccggcaagc agtgtgagta
     7261 gctccgtttt cccctgtgga ccgtcgtaat tgcacagtgc atcagtcgct ttgccacttt
     7321 ccagcatgtc ggtcagtagc gtctggaagt agtcctgcat ttccgggatc tgcgccggat
     7381 taccgccgcc gagcataatc gcgccaggcg tgcgtaaacc gtcgttcaga tcttccatca
     7441 gcagcgtaat gccggagtgg cgggtaaatt tgtcaccaaa aagggagaat gtcatagcgg
     7501 gatatctgtc gaactctgta gcaaggaagg taacaataac gctacatttg ctggggtgca
     7561 aatcggtctg ttgaagagtg agcggtgctt tatcctgcaa cgctgattag ggctgacatt
     7621 ttatcctggt tggcggcttc ccgaggtact gacgaacggg aagccggaaa agtcactgtt
     7681 gcccagccca gatgaccagc actttatcgt cgccatgctc acgaacaaag ccgtagccct
     7741 gcttcagcga aagtgtcgtt tgtttgcccg cgccaattgc gggatggcgg gcgcggaact
     7801 ggctgatttt ctgccagtgc gcgacgttgg cggcagattt accgctaaca tcctgccagt
     7861 tcatatccga acgtgtacct tgcagcggat cagaacctgt aggaccgaac ggacgcgagg
     7921 attcatcgcc ataaaagatt tgtaccgcgc ctggcgctaa tagtaataac tctgctgctt
     7981 tgtcgccccc ttcacggaac agacgggtat catgcgacga gaggtagctc aacacgttga
     8041 aaccctgcaa tttctccgcc atttgctgcc aggtcgtatc catctgcgca atacaatcga
     8101 cagctttcgc cgcctgctcc tgataatcga aattgatcat cgcatcgaag ccgtggcgat
     8161 agtagtcact ttgcatcacg ccgtggcccc aggcttcacc ggtcatccag aaaggtttgt
     8221 catctaatgc tttgtcgggg ttagcttttt tccattcgcg aagcgcggcg ctggcttcgg
     8281 ttttcagttg ctgccaagcg ggcaactcaa catgtttggc ggtatcgacc cgaaaaccat
     8341 caatcccata gtcgcggacc cactgactta accagtgggt taagtaatcg cgaggggtaa
     8401 agccgtcgat ggctttagcg tgggtatccg ttttgttttt atagaacacc ggcagaccag
     8461 aagcggtagt tgattcggtt ttgatatccg gcaaaaaggc tagcgacatg gtgagatcgt
     8521 cgaatccagg attgtcgtaa tcgccgatat cggtacggat ccagtttttt ccccaccatt
     8581 tatcccagcc tgttttgtcg ctgaaattaa tgtaatcgtt aaagctatgc caggtttgcc
     8641 cggcggcagg tttccagtcg ctccagcgtt cacccagcgt ttttttcact tcgtcaccag
     8701 aaagatataa cgcgccaaac tgatactcct gcatatccgc cagcgtggca tagccggtgt
     8761 ggttcatcac gacatcaaag agaatacgaa taccgcgctg atgtgcgcta tcaaccagcg
     8821 tccgtagatc ggcttcgttg cccatattgg catcaagatt cgtccagtcc tgtgtgtaat
     8881 aaccgtggta ggcataatgc gggaaatcgc cttttgtacc gccgccgacc cagccgtgaa
     8941 tttgctcaaa tggggcgctt atccataaag cattaacgcc caactgctgg aggtaatcca
     9001 gtttgttggt caggccgcgt aaatcgccgc cgtgaaaagt gccaatttcc gccataccgt
     9061 ctttatgacg tccgtaactc tggtcattac tgggatcgcc gttttcgaaa cgatctgtca
     9121 gcacaaagta aaccgtggcg ttatgccagt cgaaaggggc agaggcgtca gtttctgccc
     9181 gttccagcag gagtaaaccg ttgctggtag cagcgggttg taacgttatt tgaccgttct
     9241 tcactatcgc aatttgctgg ctgtaataat cccgtacggc ggctccttcc gggaaagtgg
     9301 cgctgacatc cagcgtgagc ggtaatccat cccatttcgg gcattcacgg accaggtttg
     9361 ctaccggttc ggcggcgttc tggatggaaa tcatcaatgt tggcgtaccg gagcgggtgt
     9421 ctatttgcag cgtatatttg ccgtccctga acaatcgcca ttgaggcggc gtgttgctac
     9481 aaggttgcag ggaaagcatc tgattgagtt ttatcgcatc tgcaggctgc cagcactgct
     9541 ggtcaaaatt tagcgtgagt ggacgcgtac ctttgggcaa ctgcgcgtgg ctgacaaatg
     9601 ttcccgttcc ctgttcgcta aaggcaggga accccggaga agtccagctg gcggcaacgg
     9661 cgaagccagg aaggagtgtc agaaaacagg cggcgagttt catggtggat gagtccttat
     9721 tgctgcgatt tcagacagtt tgccagcgat aagccagaca aaactcatcc ctgagcggta
     9781 agtccacagg atgagaagca agggtgagcg atcgcgcgca aaaaacggct gaattttgcg
     9841 ataaacccca cattttctgc gatttagcgc caatctgaat cgttaactcg tgacatagtt
     9901 tcagatttgg actattcctt ggggtgctct tgacagctat ttttgatatg atttgagatt
     9961 caactctcaa atttgtgaaa aatataaggt gttggaatga ttaaatccga ccaggagacc
    10021 tgatgatttt gactcccata cgacgatatg gggcgatgat tcttatgtta ctcactctgg
    10081 tgttttcgag tgaggtgtta gcgaagacgc acacaacaac agcgagtcaa aagtcccact
    10141 taactaaagc tagtaataaa caggtaagca gtaaacaaga gtattctcgc aatagtgcaa
    10201 agagtagttc acttcctgat ttgcgaaaat acccttccgg aacatcaagg aaaaaggcgt
    10261 ttctccggac cgtaatgcct tacattacca gccaaaatgc agccattact gcagaacgta
    10321 actggctgat ttcaaaacag tatcagggcc aatggtcacc tgctgagcgt gcgcgtctga
    10381 aagacatcgc caaacgctac aaggtgaagt ggtctggtaa tacgcgaaaa atcccgtgga
    10441 ataccttgct tgaacgcgta gacattatcc ctaccagtat ggtggcgacg atggctgcag
    10501 cagaaagcgg ttggggaacg tcgaagctgg cgcgcaacaa caacaatctt ttcggcatga
    10561 aatgcatgaa aggacgttgt accaatgcgc caggtaaagt gaaagggtac tcacagttta
    10621 gttctgtcaa agaatcggtg agcgcctatg tcactaacct caatacgcat ccggcttact
    10681 cttcgttccg taaatcgcgt gcgcagctgc gtaaagctga tcaggaagtg actgccacag
    10741 cgatgattca caagctgaag ggctactcga ccaaagggaa gagttataac aactacctgt
    10801 tcgcaatgta ccaggataac caacggttaa tcgcggcgca tatgtaattg tatttcctcg
    10861 agccttatcc gacttgtcgg tcggataagg cttttgatcg catcaggtgg ttgtgctgag
    10921 ttacctgatg cgacgccgac aattctcatc atcgctacaa catgacctcg ctatttacat
    10981 cgcgatactc ttttggcgtc gtgtcatatg cttttttaaa aacagagtag aaatattgca
    11041 gcgatggata accgcacatt tgcgatatct cattgatcga caaggtggtt gaaatcagca
    11101 gactgcgcgc tttctccagc ttctcagcat gaatcatggc atggatggtt tcacccacct
    11161 cttctttaaa acgcttctca agattggagc gcgagatccc gaccgcatcg agtacctgat
    11221 ccactttaat ccctttacag gcgtgattac gaatgtaatg catggcctga ataacggcgg
    11281 gatcggtcag cgaacggtaa tctgttgagc gccgttcaat gacgcgaact ggagggacca
    11341 aaatccgctg tagcggcatt tcttctttat ctaataatcg atgcaacagt tttgccgcct
    11401 gatagcccat ttgccgcgcg ccctgagcga ccgaagaaag ggcgacacgc gacagatagc
    11461 gggtcagttc ttcgttatcg atgccaatca cgcataattt ttccggtacg ggaatgtgta
    11521 gatgttcaca tacttgcaga atatgccgcg cccgggcgtc agtaacggca ataatcccgg
    11581 tttgcggtgg cagtgtttgt agccagtctg ccagccgatt ttgcgcgtgt tgccagttct
    11641 ctggtgcggt ttctaacccc tgataaacca ctccgcgata cttttcttcg gcgacaagct
    11701 gacgaaatgc atattcgcgt tcagtggccc aacgtttgcc gcttgattcc ggaagaccat
    11761 aaaaagcaaa gcggttaacg cctttctctt ttaaatgcaa aaatgcgctt tcaaccagcg
    11821 catagttatc ggtggcaatg taatgaacgg gtgggtaact ttctgcaagg tgatacgagc
    11881 cgccaacccc aacaatgggg acgtcgacat cagccagcgc ttgctcgatc tgtttgtcgt
    11941 cgaagtcggc aatgacgcca tctcctaacc agtccttgat tttatcaatg cgggcgcgga
    12001 aatcttcttc aatgaaaata tcccattccg attgtgacgc ctgtaaatat tcccctacgc
    12061 cttctactac ctgccggtca taggctttat tggcattgaa cagtaatgtg atgcggtgac
    12121 gtttagtaaa catggttctt ttcctgctga atcatgcaaa aactcaaaac cggtaatacg
    12181 ttaccggctt tgagaaaatt tttatcaaaa tcaagaacgg cgtttggttg cggagtccat
    12241 ccatactgcc agcaacagaa tcgcaccttt aacgatatac tgccagaagg tcggtacatc
    12301 catcatactc ataccgttat ccagtgaagc catgatgaat gcccccatta ccgctccagc
    12361 cacgctcccg acgccgcctg ccagactggt gccgccaatc acgcatgctg caattgcgtc
    12421 cagctcggcg atattccccg cagaaggtga accagcgcca agtcgagaac tgaggattaa
    12481 tccggcgatg gctaccatta atccgttaat agcgaacacg gcaagtttgg tgcgttcaac
    12541 gttaattccg gagagacgtg ctgcttccag attgccgccg atggcataaa tgcgtcgtcc
    12601 aaatgctgtc cgcgttgcca taaacattcc gccgagtaac agcaacgtca gcagcagaac
    12661 aggagtagga acgccacggt aatcattcaa cagccagatt gcgcctaata cgatgatagc
    12721 ggttaaagcc tggcgaccga ctactgcggt agaggctgga gactgcaaac ccaaagcctg
    12781 acggcgcatt cttccgcgcc attgccagcc aacaaaagcc attaagccaa gcgcgccaat
    12841 gatgaagccg gtgctggcgg ggagatagct ttgcccaatt tgtgacatcg cggcgctggt
    12901 gggggataca gtcgtgccgt tggtgatgcc aatgagtatg ccgcgaaatg ccaacatgcc
    12961 cgcgagagtg acaataaatg aagggacttt gcggtacgcg acccaccatc cgttccaggc
    13021 accgagaagc agtcccagaa ccaacgtcac aatgatggta agtggcaaag gccagcctag
    13081 ccagacgtca caaatcgccg cgacgccacc taatagcccc atcattgagc cgacggaaag
    13141 gtcgatttca gcagaaatta tgacgaacac cattcctacc gcgaggatgc cggtaatcgc
    13201 ggtctggcgt aacaggttgg agacgttacg ggcgcttaag taggcaccat cggtggtcca
    13261 ggtaaagaac agcatgattg cgatgatagc tgcaatcatc acgaagacct gcaaattcag
    13321 tgatttcagc ccggagaagc caccggatgt cggtacggcc aatttcactt cagacggatt
    13381 gcttttcgac atgatgttcg ctcctcaatg cggcttccat cacctgctcc tgagtcaggt
    13441 tatgatttat caggttggct tttagtttcc cttcatgcat tactagtaca cgatcgctaa
    13501 ggccgagcac ttcaggtaat tcagaagaga tgacaataac ggcaataccc tgctggacga
    13561 gttggttaat taatttgtag atctcgtatt tcgcgccaat atcgataccc ctggtgggtt
    13621 catcaagaat gagaatgcgc gggttaagta acagacagcg agcgaggatc gctttttgct
    13681 gattgccgcc gctcaaacgt ccaatagcaa ggtcggggga cgacgtttta actttgagtt
    13741 gctggattga ttccagaata catttttgct ctgccgcgtc atcaagctgg ctaatgccac
    13801 cggtaaattt attgagtgcg gcgagggtaa tatttttacc aaccgccatt accggaacga
    13861 tgccgtcgcg ctttctgtct tcggggacca tcgcaatccc ctgggcgatg gcttgctgac
    13921 agttacgaat atctacctgt ttgccatcaa tataaatttt tccttcccat tgtccgggcc
    13981 acacaccaaa caggcactga atggtctcgg tacgtccggc accaacgagt ccggcaatac
    14041 ccagtatttc gccacgtttc agggaaaacg agacatcatt aactcgttta atatgacgat
    14101 taaccggatg ccatgccgtc agatgttcaa tacgtaatat ttcatctccg gtggtatgtg
    14161 gttcattagg gtaaagcgcg gttaactctc gcccgaccat catggtgata atatcgtctt
    14221 cactcattcc ggcagcatca cgcgtaccaa tgtgctgtcc gtcgcgaata acgcaaatcg
    14281 tatcggaaat cgctttgact tcgttgagtt tgtgcgaaat ataaatacag gcgataccgt
    14341 gctgttgtag atcgcgaata atatccagta aaaccgacgt ttcctgctca gttaatgagg
    14401 ctgtcggttc atcgagaatt aacaagcgca cctgtttatt aagtgccttg gcaatttcaa
    14461 ccagttgttg ttgcccaagc cctaaatcgc caacgcgggt atcaggtgaa atggataaac
    14521 tgacctgtgc gagcagcttc tgacagcgta gcgtcatcag gtcataatcc ataatgccat
    14581 tgtgggttat ttcgttaccc aggaagatat tttccagcac ggtcaattct ttcaccaggg
    14641 ccaattcctg gtgaatgatg gcgatacctt tgcgttcggt atcgcggatg tgactcgcct
    14701 gaatctcttc tcccgcaaaa ataatttcgc cttcgtagga gccatgggga taaataccac
    14761 acagcacttt catcagcgtt gatttaccag acccattttc cccacagagt gagacgattt
    14821 cgccagcatt caaccgcaag ctgacgttat caatcgcctt cacactgccg aaggttttgg
    14881 taatgttctt catttcaagt agataaggca tagcgactcc acctaagcca attcattcac
    14941 gcggcatgga gagaaatcac gcccccgctc cgcgctgggg cgtaacgctt acagctcgct
    15001 ctctttgtgg aatccgtctt taattaccgt atctttgatg ttgtttttat tcacatcgat
    15061 cggtgtcagg aggcgggagg ggacatcttt caggccatta tttagtgaag tatctgcttt
    15121 tggctcctga ccattgccca attcaacggc aatttctgcg gcagtatttg ccagcaatgt
    15181 aataggttta tataccgtca tagtttgcgt accggcagca atacgtttaa tacctgcgag
    15241 atccgcatcc tggccagaaa ttgctacttt ccctgataaa ccttgcgcgc ttaatgcctg
    15301 aattgcccca cctgcggtgg catcgtttga ggcaactaca gcatcaattt tgttattatt
    15361 ggcggttagc gcgttttcca taattttcaa tgcgttttcc ggtaaccagc catcaaccca
    15421 ttggtcacca acgactttaa tttttccgga atcaacgtaa ggttgtaaca ctttcatttg
    15481 tccggcgcgg aacagcttag cgttgttatc taccggcgag ccgcccatca ggaagtaatt
    15541 accttgcgga acaatatcga ccagggcttt tgcctgcagt tcaccgactt tttcgttatc
    15601 gaaagaaata taaaaatcga tatccgcatc gttaatcata cggtcgtaag ctaatacttt
    15661 aatgccttct tgtttggctt cttttacaac gttacttaat acctgaccgt tatacggaat
    15721 aatgacaaga acatcgacac cccggtttat catgttttca atctgcgaca tttgtgtttc
    15781 ttcattgcca tttgcagact gtacaaatac tttcgcgccg agagattctg cctttttcac
    15841 aaagatatct cgatcttttt gccagcgttc aagacggaga tcatcaatcg ccatacctat
    15901 tttgacttct ttggcgtgtg cagcaacgtt ggtaagcagg agtgaggtgc aaagggtgag
    15961 tagaatgttc tttattttca tggtgtaggg ccttctgtag ttagaggaca gctttaataa
    16021 gtaacaatca ccgcgataaa cgtaaccaat ttttagcaac taaacagggg aaaacaatta
    16081 cagattttta tctttcgatt acgatttttg gtttatttct tgatttatga ccgagatctt
    16141 acttttgttg cgcaattgta cttattgcat ttttctcttc gaggaattac ccagtttcat
    16201 cattccattt tattttgcga gcgagcgcac acttgtgaat tatctcaata gcagtgtgaa
    16261 ataacataat tgagcaactg aaagggagtg cccaatatta cgacatcatc catcacccgc
    16321 ggcattacct gattatggag ttcaatatgc aagcctattt tgaccagctc gatcgcgttc
    16381 gttatgaagg ctcaaaatcc tcaaacccgt tagcattccg tcactacaat cccgacgaac
    16441 tggtgttggg caagcgtatg gaagagcact tgcgatttgc cgcctgctac tggcacacct
    16501 tctgctggaa cggggcggat atgtttggtg tgggggcgtt taatcgtccg tggcagcagc
    16561 ctggtgaggc actggcgttg gcgaagcgta aagcagatgt cgcatttgag tttttccaca
    16621 agttacatgt gccattttat tgcttccacg atgtggatgt ttctcctgag ggcgcgtcgt
    16681 taaaagagta catcaataat tttgcgcaaa tggttgatgt tctggcaggc aagcaagaag
    16741 agagcggcgt gaagctgctg tggggaaccg ctaactgctt tacaaaccct cgctatggcg
    16801 cgggtgcggc gacgaaccca gatccagaag tcttcagctg ggcggcaacg caagttgtta
    16861 cagcgatgga agcaacccat aaattgggtg gtgaaaacta tgtcctgtgg ggcggtcgtg
    16921 aaggttacga aacgctgtta aataccgatt tgcgtcagga gcgtgaacaa ctgggccgct
    16981 ttatgcaaat ggtggttgag cataaacata aaatcggctt ccagggcacg ttgcttatcg
    17041 aaccgaaacc gcaagaaccg actaaacatc aatatgatta cgatgctgcg acagtctatg
    17101 gcttcctgaa acagtttggt ctggaaaaag agattaaact gaacattgaa gctaaccacg
    17161 cgacgctggc aggtcactct ttccatcatg aaatagccac cgccattgcg cttggcctgt
    17221 tcggttctgt cgacgccaac cgtggcgatg cgcaactggg ctgggacacc gaccagttcc
    17281 cgaacagtgt ggaagagaat gcgctggtga tgtatgaaat tctcaaagca ggcggtttca
    17341 ccaccggtgg tctgaacttc gatgccaaag tacgtcgtca aagtactgat aaatatgatc
    17401 tgttttacgg tcatatcggc gcgatggata cgatggcact ggcgctgaaa attgcagcgt
    17461 gcatgattga agatggcgag ctggataaac gcatcgcgca gcgttattcc ggctggaata
    17521 gcgaattggg ccagcaaatc ctgaaaggcc aaatgtcact ggcagattta gccaaatatg
    17581 ctcaggaaca taatttgtct ccggtgcatc agagtggtcg ccaggagcaa ctggaaaatc
    17641 tggtaaatca ttatctgttc gacaaataac ggctaactgt gcagtccgtt ggcccggtta
    17701 tcggtagcga taccgggcat ttttttaagg aacgatcgat atgtatatcg ggatagatct
    17761 tggcacctcg ggcgtaaaag ttattttgct caacgagcag ggtgaggtgg ttgcttcgca
    17821 aacggaaaag ctgaccgttt cgcgcccgca tccactctgg tcggaacaag acccggaaca
    17881 gtggtggcag gcaactgatc gcgcaatgaa agctctgggc gatcagcatt ctctgcagga
    17941 cgttaaagca ttgggtattg ccggccagat gcacggagca accttactgg atgctcaaca
    18001 acgggtattg cgccctgcca ttttgtggaa cgacgggcgc tgtgcgcaag agtgcgcttt
    18061 gctggaagcg cgagttccgc tatcgcgggt gattaccggc aacctgatga tgcccggatt
    18121 tactgcgcct aaattgctat gggttcagcg gcatgagccg gagatattcc gtcaaatcga
    18181 caaagtatta ttaccgaaag attacttgcg tctgcgtatg acgggggagt ttgccagcga
    18241 tatgtctgac gcagctggca ccatgtggct ggatgtcgca aagcgtgact ggagtgacgt
    18301 catgctgcag gcttgcgact tatctcgtga ccagatgccc gcattatacg aaggcagcga
    18361 aattactggt gctttgttac ctgaagttgc gaaagcgtgg ggtatggcga cggtgccagt
    18421 tgtcgcaggc ggtggcgaca atgcagctgg tgcagttggt gtgggaatgg ttgatgctaa
    18481 tcaggcaatg ttatcgctgg ggacgtcggg ggtctatttt gctgtcagcg aagggttctt
    18541 aagcaagcca gaaagcgccg tacacagctt ttgccatgcg ctaccgcagc gttggcattt
    18601 aatgtctgta atgctgagtg cagcgtcgtg tctggattgg gccgcgaaat taaccggcct
    18661 gagcaatgtc ccagctttaa tcgctgcagc tcaacaggct gatgaaagtg ccgagccagt
    18721 ttggtttctg ccttatcttt ccggcgagcg tacaccacac aataatcccc aggcgaaagg
    18781 ggttttcttt ggtttgactc atcaacatgg ccccaatgaa ctggcgcgag cagtgctgga
    18841 aggcgtgggt tatgcgctgg cagatggcat ggatgtcgtg catgcctgcg gcattaaacc
    18901 gcaaagtgtt acgttgattg gtggcggggc gcgtagtgag tactggcgtc agatgctggc
    18961 ggatatcagc ggtcagcagc tcgattaccg tacggggggg gatgtggggc cagcactggg
    19021 cgcagcaagg ctggcgcaga ttgcggcgaa tccagagaaa tcgctcattg agttgttgcc
    19081 gcaactaccg ttagaacagt cgcatctacc agatgcgcag cgttatgccg cttatcagcc
    19141 acgacgtgaa acattccgtc gcctctatca gcaacttctg ccattaatgg cgtaaacgtt
    19201 atcccctgcc tgaccgggtg ggggataatt cacatctata tatctcagca attaattaat
    19261 atttagtatg aatttattct gaaaatcatt tgttaatggc atttttcagt tttgtctttc
    19321 gttggttact cgtaatgtat cgctggtaga tatggagatc gttatgaaaa cctcaaaaac
    19381 tgtggcaaaa ctattatttg ttgtcggggc gctggtttat ctggttgggc tatggatctc
    19441 atgcccattg ttgagtggaa aaggctattt tcttggcgtg ttaatgacag caacttttgg
    19501 caactatgca tatcttcgcg cagaaaaact cgggcaactg gataattttt ttacccatat
    19561 ttgccagtta gttgcgttaa tcactatcgg tctcttgttt atcggtgttt taaatgcacc
    19621 tatcaatgct tatgaaatgg tgatctaccc catcgccttt tttgtctgct tgtttggtca
    19681 aatgcgtttg tttcgctcgg tatgagcaac ataaagctct tacacattca ggaatgaaag
    19741 gaatactgtg atggacaaca aaatatcaac ctattcaccg gcctttagta ttgtgtcatg
    19801 gatagctctc gttggtggta tcgttaccta tctgttaggg ctatggaatg cagagatgca
    19861 gttaaatgaa aaaggatatt attttgccgt actggtatta ggactgtttt ctgcggcgtc
    19921 ttatcaaaag accgttcggg acaagtatga aggcataccg accacttcca tttattatat
    19981 gacctgcctg actgtcttta ttatctctgt tgcattactg atggtaggtc tgtggaatgc
    20041 gacattatta ctcagcgaaa aaggttttta tggactggct ttcttcttaa gcttgtttgg
    20101 tgcagtagcg gtgcagaaaa atattcgtga tgccggaata aacccaccaa aagaaacaca
    20161 ggttacccag gaagaataca gcgaataact cacgtaagcc cggtcagtcc aatgtgaccg
    20221 ggcttttact taactcacta atctgtttct gtcgattcgt tgtaccagca tagaaagtaa
    20281 caaactcgct gccaacgtcg cgcaaaaaat ccaaataata tccagtattg gccaattttt
    20341 aagctcaatt ccccgggtgc gcagcgcatg gataatcaag gcgtggaatc cgtatatacc
    20401 caacgaatgg cgggagatta agccaagtcc gcgaatggta cgcgtatcca gcgtgttttt
    20461 aaccagagtc aatagcgcga ttgcgcagat aaaaaccatc ggcccacagt aaggatacca
    20521 ggtatcggca aaatttccgc gccactgcaa ttcatataat gtcccgcgag agataataaa
    20581 aactcccgtc gcaaacagtg cggcgctcac ccacgacagt gctttatgct gtgtgtccat
    20641 catccctaaa gcgcggccca acatgccata cagaatgtag taaaaagtat cgccattgat
    20701 atataagtta attggcagcc attcaaaacc gtcgattttc tgcggcacgg tgtttgggtt
    20761 agcgatgata ccaatcacca ccattagcac cagcaacatt tttccgccga cgttcttcac
    20821 ctgaatcagc ggtgaaacca gataaatcac cgcaatcgcg aagaaaaacc ataagtggta
    20881 aaacactggc ttttgcagca ggtttttcag cgctaactcc acattaatgg aggtaaacag
    20941 tgcaatgtag agcagcgcga ttgcgctata aaaaaacaga cataagccga tacgcaagaa
    21001 atggcgcggc tgggcgctgc gttcgccaaa aaagagatag ccggaaatca tgaaaaatag
    21061 cggcacgctg acacgagagg cagagttcag aacattggcg atatcccagg tgacggggct
    21121 aacactatga gcattggtca cataccaggt agtggtgtga atcatcacca ccattaaaca
    21181 cgctatccct cgcaggttat caatccagta aattttgggc tgcatctgtg tctctgtatc
    21241 tggttaaaaa aagtctgacc gataaatcat tcagttggcg cactggaata atctgagttt
    21301 taacactaca gcttatagag gcttaaggaa attcgtaaga tatcagccac tataccgata
    21361 taaataataa gactcacctg caaaccagac ggtaatttaa tgatgatgaa ctctttcttt
    21421 ccggcaatgg cgcttatggt gctagtgggt tgttctacac cgtcacccgt gcagaaagca
    21481 caacgggtaa aggttgatcc tctgcgttcg ttgaatatgg aagcgttatg caaggatcag
    21541 gcggcaaaac gttataacac cggcgagcaa aaaatcgacg tcaccgcctt cgaacagttc
    21601 cagggaagct atgaaatgcg cggttatacc ttccgtaaag agcagtttgt ctgttctttt
    21661 gacgcagatg gccatttttt gcatctttcc atgcgttaag ccctgctttt tcccgtttca
    21721 tactgtatat cttccatcca gcgggtatac tgatcccttc ctttaaatcc acacgtatcc
    21781 agcacgaaat aatatgcaaa agtttgatac caggaccttc cagggcttga tcctgacctt
    21841 acaggattac tgggctcgcc agggctgcac cattgttcaa ccattggaca tggaagtcgg
    21901 cgcgggaacc tctcacccaa tgacctgtct gcgcgcgctg gggccagaac cgatggcggc
    21961 tgcttatgtt cagccttctc gtcgcccgac cgatggtcgc tacggcgaaa accccaaccg
    22021 tttacagcac tactatcagt tccaggtggt cattaagcca tcgccggaca atattcagga
    22081 gctgtacctt ggttctctga aagagctggg catggacccg actatccacg atatccgttt
    22141 cgtggaagat aactgggaaa acccgacgct gggtgcctgg ggactgggct gggaagtgtg
    22201 gctaaacggc atggaagtga cgcagttcac ttacttccag caggttggtg gtctggagtg
    22261 taaaccggtt accggcgaga tcacctacgg tctggaacgt ctggccatgt acattcaggg
    22321 cgtagacagc gtttacgacc tggtctggag cgacggcccg ctgggtaaaa ccacctacgg
    22381 cgacgtgttc catcagaacg aagtggagca gtccacttac aacttcgaat acgcggatgt
    22441 ggacttcctg ttcacctgct ttgagcagta cgagaaagaa gcgcagcagc tgctggcgct
    22501 ggaaaatccg ctgccgctgc cagcttacga gcgtattctg aaagccgccc acagcttcaa
    22561 cctgctggat gcgcgtaaag ccatctccgt caccgagcgt cagcgctaca ttctgcgcat
    22621 tcgcaccctg accaaagcag tggcagaagc atattacgct tcccgtgaag ccctaggctt
    22681 cccgatgtgc aacaaagata agtaagaggc ggctatgtct gagaaaactt ttctggtgga
    22741 aatcggcact gaagagctgc caccaaaagc actgcgcagc ctggcagagt cctttgctgc
    22801 gaactttact gcggagctgg ataacgcagg cctcgcacac ggcaccgttc aatggtttgc
    22861 tgctccgcgt cgtctggcgc tgaaagtagc taacctggcg gaagcgcaac cggatcgtga
    22921 aatcgaaaaa cgcggcccgg cgattgccca ggcgttcgac gctgaaggca aaccgagcaa
    22981 agcggcagaa ggttgggcgc gtggttgcgg tattaccgtt gaccaggctg agcgtctgac
    23041 taccgataaa ggcgaatggc tgctgtatcg cgcccatgtg aagggcgaaa gcactgaagc
    23101 actgctgccg aatatggttg cgacttcgct ggcgaaactg ccgattccga aactgatgcg
    23161 ctggggtgca agcgacgtgc acttcgtgcg tccggtgcac accgtgaccc tgctgctggg
    23221 cgacaaagtc attcctgcaa ccattctggg cattcagtcc gatcgcgtga ttcgcggcca
    23281 ccgctttatg ggcgagccgg aattcaccat cgataacgcc gatcagtatc cggaaattct
    23341 gcgtgagcgt gggaaagtca tcgccgatta cgaagaacgt aaggcgaaga ttaaagccga
    23401 tgccgaagaa gcagcgcgta agattggcgg taacgctgac ttaagcgaaa gcctgctgga
    23461 agaagtggct tcgctggtgg agtggccggt cgttctaacc gcgaaatttg aagagaaatt
    23521 cctcgcggtg ccgtctgaag cactggttta caccatgaaa ggtgaccaga agtacttccc
    23581 ggtgtatgcg aacgacggca aactgctgcc gaactttatc ttcgttgcca acatcgaatc
    23641 gaaagatccg cagcaaatta tctctggtaa cgagaaagtc gttcgtccac gtctggctga
    23701 tgccgagttc ttcttcaaca ccgaccgtaa aaaacgtctg gaagataacc tgccgcgcct
    23761 gcaaaccgtg ctgttccagc aacagctggg tacgctgcgt gacaagactg accgcatcca
    23821 ggcgctggct ggctggatcg ccgaacagat tggcgctgac gttaaccacg caacccgtgc
    23881 gggcctgctg tctaagtgtg acctgatgac caacatggtc ttcgagttca ccgacaccca
    23941 aggcgttatg gggatgcact acgcgcgtca cgatggcgaa gcggaagacg tagccgtcgc
    24001 gctgaatgag cagtatcagc cgcgctttgc cggtgatgac ctgccgtcca acccggtagc
    24061 ttgtgcgctg gcgattgctg ataagatgga tactctggcg ggtatcttcg gtatcggtca
    24121 gcatccgaaa ggcgacaaag acccgtttgc gctgcgtcgt gccgcacttg gcgtgctgcg
    24181 aattatcgtt gagaagaacc tcaaccttga tctgcaaacg ctgaccgaag aagcggtgcg
    24241 tctgtatggc gataagctga ctaatgccaa cgtagttgat gatgttatcg actttatgct
    24301 cggtcgcttc cgcgcctggt atcaggacga aggttatacc gttgacacca tccaggcggt
    24361 actggcgcgt cgtccgactc gtccggctga tttcgatgcc cgcatgaaag cggtatcgca
    24421 cttccgtacc ctggaagcag ctgcagcact ggcggcggcg aacaaacgtg tatctaacat
    24481 cctggcgaaa tctgacgaag tgctgagcga tcgcgtgaat gcctctactc tgaaagagcc
    24541 ggaagaaatt aaactggcga tgcaggttgt ggtgctacgt gacaagctgg agccgtactt
    24601 tgcggaaggt cgttaccagg atgcgctggt cgaactggca gagctgcgtg aaccagtcga
    24661 tgccttcttc gataaagtga tggtcatggt tgatgacaaa gaattgcgta tcaaccgtct
    24721 gaccatgctg gagaaactgc gcgaactgtt cctgcgcgtt gcggatattt cgctgttgca
    24781 gtaataacgc cgttattaaa aagcctgcca tctg
//
LOCUS       DABSYT020000065        24508 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.065, whole genome shotgun sequence.
ACCESSION   DABSYT020000065 DABSYT020000000
VERSION     DABSYT020000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.065"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            140..1117
                     /gene="ybeQ"
                     /locus_tag="ID725_RS15665"
                     /old_locus_tag="pgaptmp_003133"
                     /pseudo
     CDS             140..1117
                     /gene="ybeQ"
                     /locus_tag="ID725_RS15665"
                     /old_locus_tag="pgaptmp_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(1187..1669)
                     /gene="ybeL"
                     /locus_tag="ID725_RS15670"
                     /old_locus_tag="pgaptmp_003134"
     CDS             complement(1187..1669)
                     /gene="ybeL"
                     /locus_tag="ID725_RS15670"
                     /old_locus_tag="pgaptmp_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGV7851674.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1905..4487
                     /gene="leuS"
                     /locus_tag="ID725_RS15675"
                     /old_locus_tag="pgaptmp_003135"
     CDS             1905..4487
                     /gene="leuS"
                     /locus_tag="ID725_RS15675"
                     /old_locus_tag="pgaptmp_003135"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGV7851675.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4502..5083
                     /gene="lptE"
                     /locus_tag="ID725_RS15680"
                     /old_locus_tag="pgaptmp_003136"
     CDS             4502..5083
                     /gene="lptE"
                     /locus_tag="ID725_RS15680"
                     /old_locus_tag="pgaptmp_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGV7851676.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5083..6114
                     /gene="holA"
                     /locus_tag="ID725_RS15685"
                     /old_locus_tag="pgaptmp_003137"
     CDS             5083..6114
                     /gene="holA"
                     /locus_tag="ID725_RS15685"
                     /old_locus_tag="pgaptmp_003137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGV7851677.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6116..6757
                     /gene="nadD"
                     /locus_tag="ID725_RS15690"
                     /old_locus_tag="pgaptmp_003138"
     CDS             6116..6757
                     /gene="nadD"
                     /locus_tag="ID725_RS15690"
                     /old_locus_tag="pgaptmp_003138"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGV7851678.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            6781..7392
                     /gene="cobC"
                     /locus_tag="ID725_RS15695"
                     /old_locus_tag="pgaptmp_003139"
     CDS             6781..7392
                     /gene="cobC"
                     /locus_tag="ID725_RS15695"
                     /old_locus_tag="pgaptmp_003139"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGV7851679.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7652..7969
                     /gene="rsfS"
                     /locus_tag="ID725_RS15700"
                     /old_locus_tag="pgaptmp_003140"
     CDS             7652..7969
                     /gene="rsfS"
                     /locus_tag="ID725_RS15700"
                     /old_locus_tag="pgaptmp_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGV7851680.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7973..8440
                     /gene="rlmH"
                     /locus_tag="ID725_RS15705"
                     /old_locus_tag="pgaptmp_003141"
     CDS             7973..8440
                     /gene="rlmH"
                     /locus_tag="ID725_RS15705"
                     /old_locus_tag="pgaptmp_003141"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGV7851681.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8471..10372
                     /gene="mrdA"
                     /locus_tag="ID725_RS15710"
                     /old_locus_tag="pgaptmp_003142"
     CDS             8471..10372
                     /gene="mrdA"
                     /locus_tag="ID725_RS15710"
                     /old_locus_tag="pgaptmp_003142"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGV7851682.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10375..11487
                     /gene="mrdB"
                     /locus_tag="ID725_RS15715"
                     /old_locus_tag="pgaptmp_003143"
                     /gene_synonym="rodA"
     CDS             10375..11487
                     /gene="mrdB"
                     /locus_tag="ID725_RS15715"
                     /old_locus_tag="pgaptmp_003143"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGV7851683.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11498..12586
                     /gene="rlpA"
                     /locus_tag="ID725_RS15720"
                     /old_locus_tag="pgaptmp_003144"
     CDS             11498..12586
                     /gene="rlpA"
                     /locus_tag="ID725_RS15720"
                     /old_locus_tag="pgaptmp_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGV7851684.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12726..13937
                     /gene="dacA"
                     /locus_tag="ID725_RS15725"
                     /old_locus_tag="pgaptmp_003145"
     CDS             12726..13937
                     /gene="dacA"
                     /locus_tag="ID725_RS15725"
                     /old_locus_tag="pgaptmp_003145"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGV7851685.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            14047..14310
                     /gene="ybeD"
                     /locus_tag="ID725_RS15730"
                     /old_locus_tag="pgaptmp_003146"
     CDS             14047..14310
                     /gene="ybeD"
                     /locus_tag="ID725_RS15730"
                     /old_locus_tag="pgaptmp_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGV7851686.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14411..15052
                     /gene="lipB"
                     /locus_tag="ID725_RS15735"
                     /old_locus_tag="pgaptmp_003147"
     CDS             14411..15052
                     /gene="lipB"
                     /locus_tag="ID725_RS15735"
                     /old_locus_tag="pgaptmp_003147"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGV7851687.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15312..16265
                     /gene="ybeF"
                     /locus_tag="ID725_RS15740"
                     /old_locus_tag="pgaptmp_003148"
     CDS             15312..16265
                     /gene="ybeF"
                     /locus_tag="ID725_RS15740"
                     /old_locus_tag="pgaptmp_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGV7851688.1"
                     /translation="MDSNNQIESCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16474..17439
                     /gene="lipA"
                     /locus_tag="ID725_RS15745"
                     /old_locus_tag="pgaptmp_003149"
     CDS             16474..17439
                     /gene="lipA"
                     /locus_tag="ID725_RS15745"
                     /old_locus_tag="pgaptmp_003149"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGV7851689.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17540..17743)
                     /gene="tatE"
                     /locus_tag="ID725_RS15750"
                     /old_locus_tag="pgaptmp_003150"
     CDS             complement(17540..17743)
                     /gene="tatE"
                     /locus_tag="ID725_RS15750"
                     /old_locus_tag="pgaptmp_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGV7851690.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17872..18660)
                     /locus_tag="ID725_RS15755"
                     /old_locus_tag="pgaptmp_003151"
     CDS             complement(17872..18660)
                     /locus_tag="ID725_RS15755"
                     /old_locus_tag="pgaptmp_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGV7851691.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18753..19136
                     /gene="crcB"
                     /locus_tag="ID725_RS15760"
                     /old_locus_tag="pgaptmp_003152"
     CDS             18753..19136
                     /gene="crcB"
                     /locus_tag="ID725_RS15760"
                     /old_locus_tag="pgaptmp_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGV7851692.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19190..19399)
                     /gene="cspE"
                     /locus_tag="ID725_RS15765"
                     /old_locus_tag="pgaptmp_003153"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19190..19399)
                     /gene="cspE"
                     /locus_tag="ID725_RS15765"
                     /old_locus_tag="pgaptmp_003153"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGV7851693.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19574..20134)
                     /gene="pagP"
                     /locus_tag="ID725_RS15770"
                     /old_locus_tag="pgaptmp_003154"
     CDS             complement(19574..20134)
                     /gene="pagP"
                     /locus_tag="ID725_RS15770"
                     /old_locus_tag="pgaptmp_003154"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGV7851694.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            20723..22108
                     /gene="dcuC"
                     /locus_tag="ID725_RS15775"
                     /old_locus_tag="pgaptmp_003155"
     CDS             20723..22108
                     /gene="dcuC"
                     /locus_tag="ID725_RS15775"
                     /old_locus_tag="pgaptmp_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGV7851695.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIIEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(22149..22829)
                     /gene="dpiA"
                     /locus_tag="ID725_RS15780"
                     /old_locus_tag="pgaptmp_003156"
                     /gene_synonym="citB"
     CDS             complement(22149..22829)
                     /gene="dpiA"
                     /locus_tag="ID725_RS15780"
                     /old_locus_tag="pgaptmp_003156"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGV7851696.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(22798..24456)
                     /gene="dpiB"
                     /locus_tag="ID725_RS15785"
                     /old_locus_tag="pgaptmp_003157"
     CDS             complement(22798..24456)
                     /gene="dpiB"
                     /locus_tag="ID725_RS15785"
                     /old_locus_tag="pgaptmp_003157"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGV7851697.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         6147 a         6187 c         6046 g         6128 t
ORIGIN      
        1 aatgaatatt agccgcattg cgacgctcgt ttatttatac ttttctgacg cgacataata
       61 cgcataaaat aaggcttgcg caaaaaatga aacacataaa aattcaatat attttatcta
      121 caatcataag gaaatgataa tgattttcac gtcaagttgc tgcgataatt tatcaataga
      181 tgagattatc gaacgtgctg aaaaaggcga ttgcgaggct caatatattg ttgggtttta
      241 ttataatcgc gatagcgcaa ttgactctcc ggacgacgaa aaggcctttt actagctgaa
      301 gctggccgct gagcaaggtc attgtgaagc acagtattcc ttagggcaaa agtataccga
      361 ggataaaagc cgtcataaag ataatgagca agccatcttc tggctgaaaa aagctgccct
      421 acaaggccat actttcgctt ccaacgccct tggctggata ctggatcgtg gagaagaccc
      481 caactataaa gaagcggttg cctggtatca gatagccgcg gagagcggaa tgtcttatgc
      541 gcaaaataat cttgggtgga tgtacagaaa tggcaacgga gccgcacaag actatacgct
      601 ggcatttttc tggtacaaac aagctgcatt acaaggccat agtgacgcgc aaaacaatct
      661 ggccgatctt tatgaagacg gaaaaggcgt tgctcaaaac gagacactcg ccgcattctg
      721 gtatttgaaa agcgcacagc agggtaatcg gcatgctcag tttcaaattg catgggatta
      781 taacgctggc gaaggggtgg accaggacta taagcaagcg atgtactggt atctgaaggc
      841 tgccgctcag gggagcgtcg gcgcttacgt caacatcggt tatatgtata aacacggaca
      901 aggcgttgag aaagattatc aggctgcctt tgaatggttt acgaaagccg ctgaatgcaa
      961 tgacgccact gcctggtata acctggccat tatgtatcac tacggagaag gaagacctgt
     1021 cgatctccga caggctctcg acctgtatcg taaagttcag tcatccggaa ccagagatgt
     1081 cagtcaggaa attcgtgaga ctgaagattt actgtagaga taaaaacaag ggccggggaa
     1141 tttcattcgg cccatattct ctttattgct gtgcgaaatc agactattac ggctcaaacg
     1201 ggcgtctctg gaactggtca tgaccacatt tcgggcatag cgtcagcact tccggtgtgt
     1261 agatcgggag atggaagtga catttctcgc agaccagatt tcccagcccg accacttctc
     1321 cgctgtgata aaccccatga tgattgaggt cctggaaaac ttcgcgccat tcaagctgcg
     1381 ttttatcggt gatgtctgcc agctcctgcc acaagctttc tttaatcacc cgcataaaga
     1441 cgctgtcaga ttcttctttc aggctctctt catagctcat ggcgaactct tccaggtcac
     1501 gtctgacagc tcgcgtcagc tcatcgacct cggttcgcgt taactcccct gtttttatta
     1561 cgcgctcgcg cgcctgttcc accagtgcgt cgatatcacg ttcgccattg cgcaggcgtt
     1621 cgctcagtga cgcaaccagt tcacggtaat attgagcaac cttgttcatc gtttcatctc
     1681 ccgggtcgtt aactgactat aataatagac gttctttgtt ccttgctttg ctaatacgcc
     1741 cacaaactct gtaaattaat gtatgaataa tccgggcatc atttcaacgt cgagtgcgcg
     1801 aaaggctgtt ttgacgcgga cgtttgggct atgctatgcg gatctgaaaa accacctcta
     1861 acgctacttt tgtagccgta atgaaaacag gaccactggc tgccatgcaa gagcaatacc
     1921 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
     1981 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
     2041 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
     2101 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
     2161 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
     2221 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
     2281 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
     2341 aaggcctggt atataagaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
     2401 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
     2461 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatt
     2521 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
     2581 gttccgaagg cgtggaaatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
     2641 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
     2701 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
     2761 gtaacaccaa agttgctgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcaatactg
     2821 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
     2881 tattgatgga gtacggcacg ggcgcagtaa tggcggttcc ggggcacgac caacgcgact
     2941 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
     3001 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
     3061 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
     3121 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgtga ctggggtgtt tctcgtcagc
     3181 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccgaccc
     3241 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
     3301 cgattaaagc agatccacag tgggcgaaaa ctaccgttaa cggtatgcca gcgctgcgtg
     3361 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
     3421 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
     3481 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
     3541 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
     3601 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
     3661 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
     3721 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
     3781 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
     3841 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggc gtggaagggg
     3901 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcatactgcg aaaggtgatg
     3961 ttgcggcact gaacgttgat gcactgactg aagatcagaa agcgctgcgt cgcgatgtgc
     4021 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
     4081 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
     4141 atcgcgctct gatgcaggaa gcactgctgg ccgttgtccg tatgcttaac ccgttcaccc
     4201 cgcacatctg cttcacgctg tggcaggaac tgaaaggcga aggcgatatc gacaacgcgc
     4261 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
     4321 ttaacggtaa agtccgtgct aaaatcaccg ttccggtgga cgcaacggaa gaacaggttc
     4381 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
     4441 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
     4501 cgtgcgatat ctggcaacat tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
     4561 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
     4621 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
     4681 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtgctgt
     4741 atcaatctca caggataccg cgtcggtttt ccgtaatggt caaactgcgg aatatcagat
     4801 ggtgatgact gttagcgcgt cggtattgat tcctggacga gatatctacc cgattagcac
     4861 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgagca
     4921 ggaaatgatc attaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
     4981 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacta caacggatac
     5041 tccggcaacg cctgcacgcg tctccaccac gctgggtaac tgatgattcg gttgtacccg
     5101 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
     5161 gatcctctgt tattgcagga aagccaggac gctgttcgtc aggtagctgc ggcacaagga
     5221 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
     5281 ttatgccagg ccatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
     5341 aacggaccaa acgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
     5401 gacctgctgt tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
     5461 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
     5521 cttccccgct gggttgctgc gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
     5581 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
     5641 cgtttatcgc tgctctggcc agacggcaaa ctgacattac cgcgcgttga acaggcggtg
     5701 aatgatgccg cgcatttcac tccttttcat tgggttgatg ctttgttgat gggaaaaagt
     5761 aagcgcgcat tgcatattct tcagcaactg cgtctggaag gcagcgaacc ggttattttg
     5821 ttgcgcacat tacaacgtga actcttgtta ctggttaacc tgaaacgcca gtctgcccat
     5881 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
     5941 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
     6001 cgaacggaac tcaccctcaa acaagattac agtcagtcag tgtgggcaga gttggaaggg
     6061 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
     6121 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
     6181 cgtggaaacg ctggcgaatt tgattggtct gacgcgagtc acaatcatcc ctaataatgt
     6241 tcctccgcat cgtccccagc cggaagcgaa cagcgtacag cgtaaacaca tgcttgaact
     6301 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
     6361 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caagggccgg acgtgccgct
     6421 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
     6481 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
     6541 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
     6601 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
     6661 catcatccgc gaacgtttgc aaaacggtga atcatgtgat gatttattgc cggaaccggt
     6721 actgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
     6781 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
     6841 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
     6901 ctgctagatg atgtttcctt tgacctggtt ttatgcagtg aactggaacg ggcacagcat
     6961 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
     7021 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
     7081 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
     7141 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
     7201 tatcagaata ttttaatcgt cagccatcag ggcgtactta gtctgttaat tgctcgttta
     7261 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
     7321 attgatataa accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
     7381 gagaatgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
     7441 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
     7501 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
     7561 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
     7621 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
     7681 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcagggcaa
     7741 atccagcatc actgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
     7801 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggagtaga
     7861 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
     7921 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
     7981 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
     8041 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggaaaacg
     8101 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
     8161 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
     8221 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtcagtc tactgattgg
     8281 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggcg gagcagagct ggtcgctgtc
     8341 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
     8401 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
     8461 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
     8521 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
     8581 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
     8641 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
     8701 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
     8761 cgtgcagcaa acgctggacg ctttgcgcag cgtggttgat ctgaccgatg acgatattgc
     8821 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tccattccgg tgaaaactaa
     8881 cctgaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgttga
     8941 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
     9001 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
     9061 caactatgcg gcaacgcatg atatcggtaa gctgggcatt gagcgttact atgaagacgt
     9121 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
     9181 ccagttaaaa gaagtcccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
     9241 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg tagtcaccga
     9301 tccgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacttgtt
     9361 tgttgacggt atctccagca aagattattc cgccttgttg aacgacccga atacaccgct
     9421 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
     9481 ggtttcggca ttgagcgccg gggtgatcac gcgcaatacg acgctgtttg accctggctg
     9541 gtggcaactg ccaggttcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
     9601 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
     9661 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattatac
     9721 cggtatcgac ctggcggaag aacgttccgg caatatgcct acccgcgaat ggaaacagaa
     9781 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gtcagggtta
     9841 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
     9901 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
     9961 acaaccgcat gaaccgcccg ttggcgatat tcattccggt tactgggagc tggcgaaaga
    10021 cggtatgtac ggtgttgcta accgccctaa cggtacggcg cataaatact ttgccagcgc
    10081 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    10141 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    10201 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gtggcgcggg
    10261 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    10321 caccgatctg cctgcggaaa atcctgcggt tgccgcagcg gaggaccatt aatcatgacg
    10381 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    10441 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    10501 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    10561 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    10621 attattttgc tggtggcggt agatgctttc ggtgccatct ctaaaggcgc tcaacgctgg
    10681 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    10741 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa cactggcatc
    10801 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    10861 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    10921 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    10981 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    11041 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    11101 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    11161 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattttgat tctgctcgct
    11221 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    11281 ggtcgcgtca tggctggcgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    11341 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    11401 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    11461 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    11521 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacagt
    11581 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    11641 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    11701 caaaatcgtg caggatccgt ctcgatttat ccaggcggga ctggcggcaa tctatgatgc
    11761 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacac agctgacggc
    11821 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    11881 aatgatcgtg gtgcgcatta atgatcgcgg tccttacggc aacgaccgcg ttatttcgct
    11941 ttctcgcgca gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    12001 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    12061 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    12121 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    12181 tccgaccggc gcgccggtaa ccagcagcgg tttcctcggc gcaccaacga ccttagcgcc
    12241 aggtgtactg gaaggcagcg aaccgacgcc tgctccacag cccgttgtta cagctccgtc
    12301 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    12361 cggcaacttt atggtgcagg tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    12421 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    12481 gcggatccag cttggcccat tcgccaacaa agccgaagcc agtaccttgc agcaacgttt
    12541 gcaaaccgaa gcccaattac agtcatttat taccaccgcg cagtagcgta aagcaggcat
    12601 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    12661 cggtagtgca tttgctatag tagggcactt tttttaattc catcacggat gtcgtagttc
    12721 agaccatgaa taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc
    12781 tttgcacagc ctttatctct gctgcacatg ccgatgacct gaatatcaaa actatgatcc
    12841 cgggtgtacc gcagatcgat gcggagtcct acatcctgat tgactataac tccggcaaag
    12901 tgctcgccga acagaacgca gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga
    12961 ccagttacgt tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca
    13021 ctattggcaa cgacgcatgg gccaccggta acccggtgtt taaaggttct tcgctgatgt
    13081 tcctcaaacc gggcatgcag gttccggttt ctcagctgat ccgcggtata aacctgcaat
    13141 cgggtaacga tgcttgtgtc gccatggccg attttgccgc tggtagccag gacgcttttg
    13201 ttggcttgat gaacagctac gttaacgccc tgggcctgaa aaatacccac ttccagacgg
    13261 tacatggtct ggatgctgat ggtcagtaca gctccgcgcg cgatatggcg ctgatcggcc
    13321 aggcgttgat ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt
    13381 ttaacggtat tcgccagctg aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg
    13441 acggcatcaa aaccggacac actgacaaag caggttacaa ccttgttgct tctgcgactg
    13501 aaggccagat gcgcttgatt tctgcggtga tgggcggacg tacttttaaa ggccgtgaag
    13561 ccgaaagtaa aaaactgcta acctggggct tccgtttctt cgaaaccgtt aacccactga
    13621 aagtaggtaa agagttcgcc tctgaaccgg tttggtttgg tgattctgat cgcgcttcgt
    13681 taggggttga taaagacgtg tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag
    13741 ccagctatgt gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg
    13801 gaactatcaa cttccagctt gatggcaaaa cgatcgaaca acgcccgctg gttgtgttgc
    13861 aagaaatccc ggaaggtaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc
    13921 atcactggtt tggttaaaaa ttgaacactt gaaagtgtaa tttccgtccc catatactaa
    13981 gcatcagtaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    14041 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    14101 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    14161 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    14221 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    14281 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg cccctggtcg
    14341 ggtaactccc ctctcctcgc tgtgatatac ttttctccca cttttactca ttctccacgg
    14401 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    14461 gccaatctcc caggctatgc atgaattcac cgatacccgc gatgatagta cccttgatga
    14521 aatctggctg gtcgagcact atccggtatt cacccaaggg caggcaggaa aggcggagca
    14581 cattttaatg cccggtgata ttccggtgat tcagagcgat cgcggtgggc aggtgactta
    14641 tcacgggccg gggcaacagg tgatgtatgt attgcttaac ctgaaacgcc gtaaactcgg
    14701 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    14761 tatagaagcg catcctcggg ctgacgcgcc aggtgtctat gttggggaaa agaaaatttg
    14821 ctcactgggt ttacgtattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    14881 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    14941 taaaatatca caatggaaac ccgaagcgac gactaataat attgcaccac gtttactgga
    15001 aaatatttta gcgctactaa acaatcctga cttcgaatat attaccgctt aatttcatac
    15061 ctcaatggcc caatttacac tgggtcatta ctcataacac ctgctaatgc tttacatctc
    15121 cttcgcgttt gtatattttc tttatcactt gcagaaatgc gatgctacgc cttgctccgt
    15181 aactcacccc attgattaac ttattgaaat agttgattat gatgagcaaa agcgttccgt
    15241 tccaattaga cataagatta ttgtaatttt tttaaatatt atttttccat gaataaaatt
    15301 ggagtaagtg cgtggatagt aataatcaaa ttgaatcctg tttaagcaga aaatcgtcag
    15361 aaggtaaacc acaaatattt acaaccttac gaaatattga tcttaatctt ctgactattt
    15421 ttgaagctgt atatgtacat aaagggatcg ttaatgcagc gaaagtgctt aatctgaccc
    15481 cctcggcaat cagtcagtct attcagaaac tgcgcgttat attccctgac ccattgttta
    15541 ttcgcaaagg ccagggtgtc actcctaccg catttgcgat gcatctacat gagtatatca
    15601 gtcagggcct tgagtccatt cttggcgcgc tggatatcga aggaagctat gataagcaac
    15661 gaacgataac tattgcgacc accccctcgg tcggagctct ggtccttcct gtcatttatc
    15721 gggcgattaa aactcactat ccgcagcttt tactgcgcaa cccgcccatc agcgacgcgg
    15781 aaaaccaact cagtcagttt caaaccgatc tcatcataga taacatgttt tgcaccaatc
    15841 gtacggtgca acatcatgtt ctgttcaccg acaatatggt gttaatttgc cgcgagggaa
    15901 atccactact ctctttagaa gatgacagag agactatcga caacgctgca catgtactcc
    15961 tgttaccgga aggacaaaat ttcagcggtc tgcggcagag aattcaagag atgtttccgg
    16021 accggcagat cagcttcacc agctacaaca ttttgacaat cgctgcactg gttgccaaca
    16081 gtgacatgtt agcgattatt cccagccgtt tttataacct gtttagccgt tgctggccgc
    16141 tggaaaaact gccttttccg tccttaaatg aggagcaaat agatttctct atccactaca
    16201 acaaattcag cctgcgtgat cccatcctgc acggagtaat agatgttatc cgtaatgcat
    16261 tttgaaaaag tattgttaca gccagatcac agcgcagaac gataaaatgg cacaatccaa
    16321 caacaatttt acattttcgc gaccgctttg gctgctttca ggtccgtttc aatgatatac
    16381 tgccagtcgt taattcaaaa atagttgata attacaacaa tctattgaat tgaaacgctt
    16441 tccttcgtaa ttcgcaactg gaacacgcac gctatgagta aacccattgt gatggaacgc
    16501 ggtgttaaat accgcgatgc cgataagatg gcccttatcc cggttaaaaa cgtggcaaca
    16561 gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa tcaagcttcc agcggactct
    16621 acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag
    16681 gaagcctcct gccctaacct ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc
    16741 ctgggcgcta tttgtacccg ccgttgtccg ttctgtgacg ttgcccacgg tcgcccggta
    16801 gctcctgatg ccaatgagcc agtgaaactg gcgcagacca ttgccgatat ggcgctgcgt
    16861 tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg atggcggtgc ccaacacttt
    16921 gcggattgta ttactgccat tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg
    16981 ccggatttcc gcggtcgtat ggatcgtgct ctggatattc tgactgcaac gcccccagat
    17041 gtgttcaacc ataacctgga aaacgtaccg cgtatttacc ggcaggtacg gcctggcgca
    17101 gattacaact ggtcgctgaa gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg
    17161 accaagtctg gtctgatggt gggtctgggt gaaaccaatg aagaaattat tgaggtaatg
    17221 cgcgacctgc gccgtcatgg tgtgacgatg ttaacgctgg ggcaatattt gcagccaagc
    17281 cgccatcacc tgccggttca acgttacgtt agcccggatg agttcgacga aatgaaagcc
    17341 gaagcgctgg cgatgggctt tacccatgct gcatgcggtc cgtttgtccg ctcttcttac
    17401 cacgccgatt tgcaggcgaa agggatggaa gttaagtaat cctgtaataa ttacttacat
    17461 ctgtctgata aaaaacccgc tttttgaagc gggttttttg tatcgaacaa gatattgagg
    17521 gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga agatcaacgt
    17581 ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac cctttaatgg
    17641 ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc agaacgacca
    17701 gcgccgcaac taccagcagt ttggtaatac taatctcacc catagatacc ttcttgacat
    17761 ataaccggca acagaacgcg ccttaaaaac caaagaatat taacggtcat tcgacgcgcg
    17821 cacacaatag caatctgtaa caaggtgaat caagcgccga gtaaaaaaac atcataataa
    17881 ttgcggcggc gcaaagcgac ggttgtttaa gacgggcagt tgcgcgcgca cctgacgcac
    17941 acgttcgggc gtcacttccg ccataatgag tgcaggcatt tctgacgctg ccgcaatggt
    18001 gacgccaaag ggatctataa tccggctttg cccgatattt ttgttcccgc actcccccgc
    18061 cgccaccata taacaggtgg tatccagcgc acgagcggca agcaacgttg accagtggtg
    18121 ctctttgagc ggcccgcgaa cccaggcggc aggaagtacc aggatttcag ctccctgtaa
    18181 tgcctgtgcc agcgccagct ctggaaagcg taagtcataa caggtcatca gaccgacctt
    18241 catcccctcc acctcgagta acggagcaat ttcattacca gcatcaacac ggcgtgattc
    18301 ctgaatggca aatgcatcat agagatgcaa tttggcataa cgggcgacga tgtttcctgc
    18361 ctgtagcgcc accagcatat tccatgcgcg ccccggcgtt gaaggaacat gaatcgtcag
    18421 aattgtcgtc atcatgttac gtttactttc tcgccgtaaa cgtccgagga attcgccttc
    18481 cagcagctgt gctgatttaa ccgatagatc tgcatcatga tcatcgcgcg ccagcaatgc
    18541 ttccggcagg acaaacagcg atacgtcgtt ttccgccgcc tgcgccatca acgaggcaca
    18601 aatctcagcg ttcttttccc acacagatgt aacagcaaac tgtcctgcag caaccagcat
    18661 tgccacctcc tgtcagagtc gtaaaattcg ccaacgacga ttattcggcg ttacacttat
    18721 cactcataca aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta
    18781 ttggcggtgg tacgggaagc gtggcgagat ggctgttaag tatgcgattt aacccgctgc
    18841 atcaggcgat tccgttgggg acgctggcag caaacctgat tggggcattc atcataggaa
    18901 tgggattcgc ctggttcagc aggatgacga acattgatcc agtgtggaaa gtattaatca
    18961 ccaccggatt ttgtggcggt ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt
    19021 tgttacaaga gggccgcttt ggctgggcat tactgaacgt tttcgtcaac cttctggggt
    19081 cttttgccat gaccgcactg gcattctggc tgttttcggc ctcaaccgca cactaaagac
    19141 caaaaaaaac ccgctgatta agcgggtttt gaattcttgc tgacgtatct tacagagcga
    19201 ttacgtttgc agcagaaggg cctttggcac cgttagtgat ttcgaactct acgcgctgac
    19261 cttcagcaag agttttaaaa ccattagtct ggattgcaga gaagtgtacg aacacgtctt
    19321 tgctgccgtc ttccggagta atgaaaccga atcctttgga ctcattaaac cacttaacgt
    19381 tacctttaat cttagacatc aaaattacct ttacatgaaa aatagacaca aatgctgtgt
    19441 cggttaccag tacaccaatt gtggtacgct ttgtccagtg caactttact aaaaagtgat
    19501 aaaaatcgcg ttatttttga tgttacttat tttagttatt gattttaaag aagttactaa
    19561 aacttcattt gtctcaaaac tgaaagcgca tccaggcaaa gtacacattg ccattgttgt
    19621 aggtacccgg aatgtaggtc atctgaaaag taactgggcc ataacccacg gaggccaatg
    19681 gcagtagaac cgggagaggg atgtaattcc agttatcgcg tgccgttacg ccagcggtga
    19741 atcccagacc taaatgaaaa ttttcatccg ccagcggtcg ccaggtactt tcccatccgt
    19801 atccggcaat cggttcccat ttgttccacg agtccttaaa tgccatggca tacaggccat
    19861 gccagtttcc tttttcatcc caacgcgaca ggccaaaacc gccaccccac ggtcgctcgt
    19921 tatagcgatc ggttttttct ttgtcgtaag cgaaacgtgc atgccaggtg atggcaggaa
    19981 tatataaatc ataatgttca ggctgttgcc aggtttgtac aatattttct ctaaacgttg
    20041 tcatccactc atctgcgtta gcaaaaactt taccaacgct gattaactga ataaaaacaa
    20101 aggaaaagat agcgacatat ttactcacgt tcatttgtga ccataaaaca tttatcaaaa
    20161 atctactact agcatagcaa agctacaatt aacataacct taacagcccc aacataaaga
    20221 ataatctgaa tagctgctct atttatttcc gcttgttcaa gttaaatatt tccgcgcatc
    20281 gatttaaaat gacacatcgt tttctgcgat gggaatagtc ataaaagaaa aagctagttg
    20341 tttctgacaa caaaatgcaa ccaagaaaaa atgacttatt ttttaataag atactaaaaa
    20401 ccatcttaac agggaatata ttatatatcg tcaacccacg cccattgcag attatttttt
    20461 gagcacacaa ccgcagctaa caccattgca attaacaaat ttgcatcaat cctccatcaa
    20521 tttacacaca ttattatgtg ataattgcca accgctaaat atgcgttttg ttatctacgt
    20581 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcat
    20641 cgccaattta ttgggcatat tttttcctta agctttagga attttttatt atttactttg
    20701 gggcctggag acaggaaaaa ttatgctgac attcattgaa ctccttattg gggttgtggt
    20761 tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg tgttatttgt
    20821 cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt taccgtccag
    20881 ccaggcttca acaggctaca gcgccacgga tatcattgaa tacgttaaaa tattactaat
    20941 gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg ccgcttacat
    21001 gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat tgcagtatat
    21061 taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctaa tgtctctggc
    21121 cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc cggtgatggt
    21181 aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg cggcgattat
    21241 tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaaa tgtcgctgat
    21301 tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg gcatggcgat
    21361 cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc atgaaatgtt
    21421 agatgtcagt gaaatcacca ccactgcccc tgcgttttat gccattttgc cgttcacgcc
    21481 gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca tcatcactat
    21541 tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct ttaataccca
    21601 gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt ttgctaacgt
    21661 ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca tcggctttat
    21721 tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc tgatgctggt
    21781 attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg cgccgtttta
    21841 tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc cggcgtattt
    21901 gactatcccg atgctgcagg cgtcaaacct gggccgtacc ctttcgcccg tttctggcgt
    21961 agtcgttgcg gttgccggga tggcgaagat ctcgccgttt gaagtcgtaa aacgcacctc
    22021 ggtaccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga tggtgccagg
    22081 aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt tcctccggct
    22141 ttatcatgtc acccactgtg gtatatgcgt tgcggtctgc caactttgcc gtgaacaatt
    22201 tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc agtggtgcga
    22261 ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg ctctttaaac
    22321 agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggcagttc gtctttgggt
    22381 tcaccgcgag cataggcatt aaacatctca tcaatttgct tctggctggc gctatcaata
    22441 ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag ccgttcatag
    22501 gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga caccgtttcc
    22561 atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg caccagttca
    22621 tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat tagccccggc
    22681 ttaaaacgct cgatcatcat tcgggcctgc gccaggtttc ccgccagtaa tatctgactg
    22741 aatccgggaa tgtgacgaat atattcagca tgcatctctg ccagcggcgt ttcgtcctca
    22801 acgatcaata gggttaatgg agctgtcatt tggtttcact ttcggaatat agattgaaaa
    22861 taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc gcgttacgta
    22921 gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac gcgtactgac
    22981 cccctgctca aatattttgt ctcgtagaga ctctggaacg ccgcagccct gatcggcgac
    23041 ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct tgtttccttc
    23101 atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgcccacaa tggctgcaaa
    23161 ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga caatgatcat
    23221 ttttagcccc agttcccggg cgcgctgcac tttaccaaaa agcagccccg ccacctggcg
    23281 atcggcaaac gcctcgcgca ggctgtcaat aagctgttgc tgggcctgag actccccctg
    23341 caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga gcgtcgacat
    23401 ccaattgaga tgctcgtgtc gcaatgtacg aaggctctca acgtattgtt ttatttgcgt
    23461 cagttgcgca ttgagggtgg aaatttcgtc tttactgcga aagctgataa tggcccccag
    23521 caaatcatca cctgaacgaa tagcttcccg attggcaata acgctcagac cgttaaagtt
    23581 agccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg cgggcctgac
    23641 cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc ccagcatctt
    23701 tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa tcagcccttc
    23761 ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt gctttggctc
    23821 catgcccatc atttgccgac ggatatgcgc tgccaggaac cacgacagca acatcagaat
    23881 ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc gccagctatc
    23941 gattttactc accaggtagc caatcgacac cacgccgatg acttttccat cgtcatcaaa
    24001 gattggcgtt ttggcgcgca tcgccatccc cattgaccct ttcccggtaa tgaagtagct
    24061 ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa ttttctccgg
    24121 attaggatgg taaaggcgga tcgagtgccg gtccccaatc accacataat caaaatcggt
    24181 atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg ttttcacctc
    24241 agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca tgtcgcgtac
    24301 atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg ctgcaatgac
    24361 aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca gtctttggaa
    24421 aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg gttgtcccga
    24481 gcctctttga cggatgtaat taaaatgt
//
LOCUS       DABSYT020000066        24051 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.066, whole genome shotgun sequence.
ACCESSION   DABSYT020000066 DABSYT020000000
VERSION     DABSYT020000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.066"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            139..3891
                     /gene="yfaL"
                     /locus_tag="ID725_RS15790"
                     /old_locus_tag="pgaptmp_003158"
     CDS             139..3891
                     /gene="yfaL"
                     /locus_tag="ID725_RS15790"
                     /old_locus_tag="pgaptmp_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGV7851698.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFPANGVAAAIDLCQGYNIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NIVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4032..4754)
                     /gene="ubiG"
                     /locus_tag="ID725_RS15795"
                     /old_locus_tag="pgaptmp_003159"
     CDS             complement(4032..4754)
                     /gene="ubiG"
                     /locus_tag="ID725_RS15795"
                     /old_locus_tag="pgaptmp_003159"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGV7851699.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4901..7528
                     /gene="gyrA"
                     /locus_tag="ID725_RS15800"
                     /old_locus_tag="pgaptmp_003160"
     CDS             4901..7528
                     /gene="gyrA"
                     /locus_tag="ID725_RS15800"
                     /old_locus_tag="pgaptmp_003160"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGV7851700.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7736..9364
                     /gene="yfaA"
                     /locus_tag="ID725_RS15805"
                     /old_locus_tag="pgaptmp_003161"
     CDS             7736..9364
                     /gene="yfaA"
                     /locus_tag="ID725_RS15805"
                     /old_locus_tag="pgaptmp_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HGV7851701.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            9361..9984
                     /gene="yfaT"
                     /locus_tag="ID725_RS15810"
                     /old_locus_tag="pgaptmp_003162"
     CDS             9361..9984
                     /gene="yfaT"
                     /locus_tag="ID725_RS15810"
                     /old_locus_tag="pgaptmp_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HGV7851702.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10008..14521
                     /locus_tag="ID725_RS15815"
                     /old_locus_tag="pgaptmp_003163"
                     /pseudo
     CDS             10008..14521
                     /locus_tag="ID725_RS15815"
                     /old_locus_tag="pgaptmp_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            14522..16171
                     /gene="yfaQ"
                     /locus_tag="ID725_RS15820"
                     /old_locus_tag="pgaptmp_003164"
                     /pseudo
     CDS             14522..16171
                     /gene="yfaQ"
                     /locus_tag="ID725_RS15820"
                     /old_locus_tag="pgaptmp_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            16176..16952
                     /gene="yfaP"
                     /locus_tag="ID725_RS15825"
                     /old_locus_tag="pgaptmp_003165"
     CDS             16176..16952
                     /gene="yfaP"
                     /locus_tag="ID725_RS15825"
                     /old_locus_tag="pgaptmp_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGV7851703.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDSSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17227..20076
                     /gene="rcsC"
                     /locus_tag="ID725_RS15830"
                     /old_locus_tag="pgaptmp_003166"
     CDS             17227..20076
                     /gene="rcsC"
                     /locus_tag="ID725_RS15830"
                     /old_locus_tag="pgaptmp_003166"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGV7851704.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20276..20926)
                     /gene="rcsB"
                     /locus_tag="ID725_RS15835"
                     /old_locus_tag="pgaptmp_003167"
     CDS             complement(20276..20926)
                     /gene="rcsB"
                     /locus_tag="ID725_RS15835"
                     /old_locus_tag="pgaptmp_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGV7851705.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20943..23615)
                     /gene="rcsD"
                     /locus_tag="ID725_RS15840"
                     /old_locus_tag="pgaptmp_003168"
     CDS             complement(20943..23615)
                     /gene="rcsD"
                     /locus_tag="ID725_RS15840"
                     /old_locus_tag="pgaptmp_003168"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGV7851706.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5887 a         5852 c         6544 g         5768 t
ORIGIN      
        1 atgacgcact gatactattc atcaaataat aacaaaatag ctattgcatc gggttggaca
       61 atttacaaaa gaaagataac ccatattgta taatgcgttt aaatatgccg tcttatataa
      121 aatgatggtc agattaatat gcgggttatc tttctacgca aggagtattt atctctactc
      181 ccgtcaatga ttgcatctct tttccctgct aacggtgtcg cggcggccat tgatttatgc
      241 cagggatata atatcaaagc gagttgtcac gccagcaggc aaagcctttc aggcattacg
      301 caggtctgga gtattgccga tgggcaatgg ctggtttttt cggatatgac caataatgcc
      361 agcggtgggg ccgtattttt gcaacaagga gcggaattta cattatcacc agaaaatgaa
      421 actggaatga ctctgtttgc caataacatc gtttcaggag aatataataa cggcggggca
      481 atatttgcta aagaaaactc aacgctgaat cttacggatg ttattttttc tggtaacgtc
      541 gcaggcggct atggtggcgc aatctattct tctggtacta acgataccgg tgccatcgat
      601 ttacgtgtca ctaacgccgt gtttcgcaat aacatcgcta atgacggcaa aggtggtgca
      661 atttatacca tcaataatga tatctattta agtgatgatg tttttaacaa taaccaggca
      721 tatacatcaa caagttacag tgatggcgat ggcggcgcaa tcgatgtcac agataataat
      781 agcgacagca agcatccttc aggttatacg ataataaata acactgcctt tacaaataac
      841 actgccgaag gttatggcgg ggcgatatat accaatagcg cgacggctcc ctatcttatt
      901 gatatttctg ttgatgacag ctacagccag aacggaggcg tgttagtcga tgagaacaat
      961 agcgcagcag gctatggaga tggtccttcc tctgcggcgg gtggctttat gtatctcggc
     1021 ttaagtgaag ttacctttga tattgccgac ggaaaaacgc tggttattgg caatacagag
     1081 aatgacggag ctgttgactc tattgctggt accgggttaa tcaccaaaac aggttccggc
     1141 gatctggtac ttaatgcaga taacaatgac tttactggtg agatgcagat tgaaaacggt
     1201 gaagttaccc tgggccgcag caactccctg atgaatgtcg gcgatacgca ttgccaggac
     1261 gatccgcaag actgctacgg tctgacgata gggagtattg ataagtacca gaatcaggca
     1321 gagctaaatg ttggctccac ccaacaaacc tttgcgcact cattgacggg ctttcagaat
     1381 ggcactttaa atatcgatgc tggtggcaat gttactgtta atcaaggcag ttttgctggc
     1441 accatcgaag gtgctggtca gctcaccatt gcgcaaaacg gcagctatgt gctggcgggg
     1501 gcgcagtcga tggcgctaac cggcgatata gtggtggatg ctggtgcggt gctttcgctg
     1561 gaaggcgacg cggcagatct tgccgctctc caggacgatc cgcagtcgat cgtgttaaac
     1621 ggcggtatgc tcgatctctc tgatttctcc acctggcaga gcggtacatc atacaaagat
     1681 ggccttgaag tcagtggcag cagcggaacg gttatcggca gtcaggatgt ggtagatctt
     1741 gcaggcggaa acgatatgca tatcggcggc gacgggaaag atggcgtcta cgtggtgatc
     1801 gatgcgggtg acgggcaggt cagcctggca aatgacaatc aatacctcgg cacaacgcaa
     1861 atcgcttccg gtacgctgat ggtgagcgac aactcgcagc ttggatatac ccattataac
     1921 cgccaggtta tctttaccga taagccacaa gaaagcgtga tggagattac tgccaatgtc
     1981 gatactcgct ctacaacgac tgagcatggg cgtgatattg aaatgcgcgc cgacggtgaa
     2041 gtggcagttg atgcgggggt agacacgcag tggggcgcac tgatggctga cagcagcggg
     2101 cagcatcagg atgagggtag cacattgact aaaacggggg cgggtacact ggagctgacc
     2161 gccagcggta caacgcagtc agcggtgaga gtagaagagg gcacgctgca aggtgatgtt
     2221 gcggatatct tcccttatgc ttcgtcgcta tgggtcggtg acggggcaac gttcgttact
     2281 ggcgcggatc aggatattca gtcaattgat gctacttcca gcggcactat cgacatcagc
     2341 gatggtacgg ttttgcgcct gaccgggcag gatacttccg tcgcccttaa tgcctcactg
     2401 tttaactgcg atgggacgct ggtgaatgcc accgatggtg tgacgttgac aggtgagctt
     2461 aataccaacc ttgaaactga cagcctgact tatctttcca acgtgacggt taatggcaat
     2521 ctgaccaata cgtccggtgc ggttagcctg caaaatggcg tcgctggcga tacgctgacg
     2581 gtaaacggtg attataccgg cggcggtacg ctactgctcg atagcgaatt aaacggcgat
     2641 gactcggtaa gcgatcaatt ggtgatgaac ggtaatactg ctggcaacac aactgtggtg
     2701 gttaactcca ttacagggat tggtgagccg acatcgacag gcattaaagt ggttgatttc
     2761 gcagctgatc ccacgcagtt tcaaaacaat gcgcagttca gtctggcagg cagcggctac
     2821 gtcaatatgg gagcgtatga ctacacgctg gtggaagata acaacgactg gtatctgcga
     2881 tcgcaagaag taacgccgcc atcgccacct gatccagacc cgactcccga tcctgatccc
     2941 acgcaggatc ctgatccaac acccgacccg gaacctacgc ctgcttacca gccggtgttg
     3001 aatgccaaag ttggcggtta tctcaataac ctgcgggcgg caaatcaggc gtttatgatg
     3061 gagcgacgcg atcacgcagg tggcgatggt cagacgctga atttacgtgt tatcggcgga
     3121 gattatcatt acacagcagc ggggcaactg gctcaacatg aagacacttc tacggtgcaa
     3181 cttagcggcg atctgtttag cgggcgctgg ggcacggatg gcgagtggat gcttgggatt
     3241 gttggtggct acagcgataa ccagggcgac agccgctcga gtatgaccgg aactcgcgcc
     3301 gataaccaga accacggtta tgcggttggg ctgacctcaa gctggtttca gcacggtaag
     3361 cagaagcagg gggcctggct ggataactgg ttgcagtacg cgtggtttag caatgatgtt
     3421 tctgaacatg aagatggcgt ggatcattac cattcgtcgg ggattatcgc ctcgctggaa
     3481 gcggggtatc agtggttacc ggggcgtggt gtggtgattg aaccgcaggc gcaggtgatt
     3541 tatcagggcg tgcagcagga tgattttacc gccgctaacc gtgcgcgcgt gtcacaatcg
     3601 cagggtgatg atattcagac gcggctgggt ttacacagcg aatggcgtac cgctgttcat
     3661 gtcataccaa cattagatct gaattattat cacgatcccc attcgacgga aattgaagaa
     3721 gatgccagca ctatcagtga cgatgcggtg aagcaacggg gtgaaataaa agtgggagtc
     3781 acgggcaata tcagtcagcg agtttcgctg cgtggtagcg tggcgtggca gaaagggagt
     3841 gatgattttg cccagacggc agggtttttg tcgatgacgg tgaaatggta atcgctgtct
     3901 gaccatcact attgctggat gcggcgtaaa cgcctaatca ggcctacgga gagcatttgt
     3961 aggcctgata agacgcggca agcgtcgcat caggcattgg cgcggttgcc ggatgaatcc
     4021 aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt tcacatccac gccggggccg
     4081 agtttaaaag tattagtgat cgggttgtaa tgtagcccgg ttatatgtcg ctctttaagg
     4141 cttgtctgat ctacccagcc cagcagttct gccggtttaa taaacttctt cacatcatgt
     4201 gtacctttag gcaccatgcg caaaatatat tccgcgccga ctaccgccat cagccatgac
     4261 ttgccgttgc ggttaagcgt cgagaaaaag acatcgccgc ccggtttcac cagttgcgca
     4321 caggctctga ccactgactg cggatcaggg acgtgctcca gcatctccat gcaggtcacc
     4381 acatcatact gcccggcatg ttttgccgcg tgctcttcca cggtttcctg cacgtaatcc
     4441 acctgaatgc cgctttccag cgcgtgcagc tttgccacct gcaatggctc aaagcccata
     4501 tccagaccgg tcaccgtcgc gccttcgcgc gccatactct cggccagaat gccgccgcca
     4561 caaccgacat cgagcacctt tttgccaaat aagccgccag cacgctcggc aatatagctc
     4621 agacgcagcg ggttaatgcg gtgcagcggt ttgaactcac cttccagatc ccaccagcgg
     4681 gaggcgacgg cttcaaattt agcgatctct tcgtggtcta cgttatggtt taccggcgat
     4741 ttttcggcat tcattggcac ttctactccg taattggcaa gacaaacgag tatatcaggc
     4801 attggatgtg aataaagcgt ataggtttac ctcaatctgc gcggctgtgt tataatttgc
     4861 gacctttgaa tccgggatac agtagaggga tagcggttag atgagcgacc ttgcgagaga
     4921 aattacaccg gtcaacattg aggaagagct gaagagctcc tatctggatt atgcgatgtc
     4981 ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc ctgaagccgg tacaccgtcg
     5041 cgtactttac gccatgaacg tactaggcaa tgactggaac aaagcctata aaaaatctgc
     5101 ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat ggtgactcgg cagtttatga
     5161 cacgatcgtc cgtatggcgc agccattctc gctgcgttac atgctggtag acggtcaggg
     5221 taacttcggt tccatcgacg gcgactctgc ggcggcaatg cgttatacgg aaatccgtct
     5281 ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa gagacggtcg atttcgttga
     5341 taactatgac ggcacggaaa aaattcccga cgtcatgcca accaaaattc ctaacctgct
     5401 ggtgaacggt tcttccggta tcgccgtagg tatggcaacc aacatcccgc cgcacaacct
     5461 gacggaagtc atcaacggtt gtctggcgta tatcgatgat gaagacatca gcattgaagg
     5521 gctgatggaa cacatcccgg ggccggactt cccgacggcg gcaatcatta acggtcgtcg
     5581 cggtattgaa gaagcttacc gtaccggtcg cggcaaggta tatatccgcg cccgtgctga
     5641 agtggaagtt gacgccaaaa ccggacgtga aaccattatc gtccacgaaa ttccgtatca
     5701 ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg gtaaaagaaa aacgcgtgga
     5761 aggcatcagc gcgctgcgtg acgagtctga caaagacggt atgcgcatcg tgattgaagt
     5821 gaaacgcgat gcggtcggtg aagttgtgct caacaacctc tactcccaga cccagttgca
     5881 ggtttctttc ggtatcaaca tggtggcatt gcaccatggt cagccaaaga tcatgaacct
     5941 gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa gtggtgaccc gtcgtactat
     6001 tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt gaagcattag ccgtggcgct
     6061 ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg ccgacgcctg cagaagcgaa
     6121 aactgcgctg gtggctaatc cgtggcagct gggcaacgtt gccgcgatgc tcgaacgtgc
     6181 tggcgacgat gctgcgcgtc cggaatggct ggagccagag ttcggcgtgc gtgatggtct
     6241 gtactacctg accgaacagc aagctcaggc aattctggat ctgcgtttgc agaaactgac
     6301 cggccttgag cacgaaaaac tgctcgacga atacaaagag ctgctggatc agatcgcgga
     6361 actgctgcgt attcttggta gcgccgatcg tctgatggaa gtgatccgtg aagagctgga
     6421 gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa atcaccgcca acagcgcaga
     6481 catcaacctg gaagatctga tcacccagga agatgtggtc gtgacgctct ctcaccaggg
     6541 ctacgttaaa tatcagccgc tttctgaata cgaagcgcag cgtcgtggcg ggaaaggtaa
     6601 atctgccgca cgtattaaag aagaagactt tatcgaccga ctgctggtgg cgaacacgca
     6661 cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat tcgatgaaag tttaccagtt
     6721 gccggaggcc actcgtggcg cgcgcggtcg tccgatcgtc aacctgctgc cgctggagca
     6781 ggacgaacgt atcaccgcga tcctgccggt gaccgagttt gaagaaggcg tgaaagtctt
     6841 catggcgacc gctaacggta ctgtgaagaa aaccgtcctc accgagttca accgtctgcg
     6901 taccgccggt aaagtggcga tcaaactggt tgaaggcgat gagctgatcg gcgttgacct
     6961 gaccagcggc gaagacgaag taatgctgtt ctccgccgaa ggtaaagtgg tgcgctttaa
     7021 agagtcttct gtccgtgcga tgggctgcaa caccaccggt gttcgcggta ttcgtttagg
     7081 cgaaggtgat aaagtcgtct ctctgatcgt gcctcgtggc gatggcgcaa tcctcaccgc
     7141 aacgcagaac ggttatggta aacgtaccgc agtggcggaa tacccgacta agtcgcgtgc
     7201 gacgaaaggg gttatctcca tcaaggttac cgaacgtaac ggtttagttg ttggcgcggt
     7261 acaggtagat gactgcgacc agatcatgat gatcaccgat gccggtacgc tggtacgtac
     7321 tcgcgtttca gaaatcagca tcgtgggccg taacacccag ggcgtgatcc tcatccgtac
     7381 tgcggaagat gaaaacgtag tgggtctgca acgtgttgct gaaccggttg acgaggaaga
     7441 tctggatacc atcgacggca gtgccgcgga aggggacgat gaaatcgctc cggaagtgga
     7501 cgttgacgac gagccagaag aagaataatt ttacttcttc atgccaaaag ggagccatct
     7561 cccttttttg aattgaaaag tccaggctgc aaagtctggg cttttgtcgt attagggcgc
     7621 ggtaaagttt ggctgtgtcc gtaaaaaatg gctggctata cacaaggaat gtggcaatga
     7681 gtggtgaaaa aaagcgaaag gctggcggtt ctatggtctt gtaggttttg gcgcaatagc
     7741 actgctttcc gctggcgtct gggcgttgca atatgctggc agtgggccag aaaaaacgtt
     7801 gtcgccgctg gtggtgcaca ataatttgca aattgatctc aatgagccgg acctctttct
     7861 cgacagcgat tctctaagcc agcttcccaa agatctcctc accattccgt ttctccacga
     7921 tgttctgagt gaagatttcg ttttctatta tcagaatcat gccgatcgtc tgggcattga
     7981 aggcagcatt cgtcgcattg tctatgaaca cgatctcacg ctgaaagata agctcttttc
     8041 gtcactctta gatcagcccg cgcaggcggc gctatggcac gataaacaag gccatctttc
     8101 acattacatg gtgctgatcc aacgcagtgg tttaagcaaa ctgctggagc cattgttatt
     8161 tgccgctacc agcgacagcc agttaagcaa aacggaaatc agtagcatca agataaatag
     8221 tgaaactatt cctgtttatc agttgcgcta taacggcaat aacgccctga tgttcgcgac
     8281 atatcaggac aagatgctgg tgttttccag cacggatatg ctgtttaaag atgatcagca
     8341 ggataccgaa gccacggcga tcgcaagtga tttgttgagc ggcaaaaaac gctggcaagc
     8401 cagctttggc ctggaagagc gtgctgccga aaaaacgcca gtacgccagc gcatcgtggc
     8461 cagcgccagg ttgctgggtt ttggctacca gcggttaatg ccttcttttg ctggcgtacg
     8521 cttcgaaatg ggtaacgacg gctggcacag ttttgtggcg ttaaatgatg aatccgccag
     8581 cgtagatgcc agtttcgatt ttacgccggt ctggaacagt atgcctgccg gggccagctt
     8641 ctgtgtggcg gtgccgtatt cacacggtat tgccgaagag atgctttcgc acatcagcca
     8701 ggaaaacgac aagttgaatg gggcgttgga cggtgccgcg gggctgtgct ggtatgaaga
     8761 ctcaaaattg caaaccccgc tgtttgtcgg tcagtttgat ggcactgccg aacaggcgca
     8821 attgccaggg aaactgttta cgcaaaatat tggtgcgcac gaaagcaaag cgccagaagg
     8881 tgttttgccg gtaagccaga ctcagcaggg cgaagcgcaa atctggcgtc gcgaagtgag
     8941 ttcccgatac ggtcagtatc cgaaagcgca ggcggcgcaa cccgatcaat taatgtcgga
     9001 ttattttttc cgcgtgtcgc tggcgatgca aaacaaaacg ctgcttttct ccctcgatga
     9061 cacgctggtt aataacgccc tgcaaacact gaataaaacc cgcccggcaa tggtggatgt
     9121 aatacccact gatggcatcg ttccgctcta tatcaatcca caaggcgtgg cgaaactgct
     9181 gcgtaacgaa acgttgacca gtctgccgaa gaatctcgaa ccggtttttt ataacgccgc
     9241 acaaacttta ttaatgccga agctggacgc tttatctcaa caaccgcgtt atgtcatgaa
     9301 gctggcccag atggaacccg gtgccgcctg gcagtggcta cccataatct ggcagccgct
     9361 atgaggcacg ggctgctggc gctgatttgc tggctgtgtt gtgttgttgc ccatagcgaa
     9421 atgctgaatg tcgaacaatc cgggctgttt cgcgcctggt ttgtacgcat tgcacaagaa
     9481 cagctccgcc aggggccaag tccacgctgg tatcagcagg attgtgcggg cctggtgcga
     9541 tttgcggcga acgagacgct gaaagttcac gacagtaaat ggctaaaaag taacggttta
     9601 tctagccagt atttgccgcc agagatgacg ctaacacctg aacagcgtca actggcgcaa
     9661 aactggaatc aggggaacgg gaaaaccggc ccctacgtga ccgcgattaa tttgattcag
     9721 tacaacagcc agtttattgg ccaggacata aaccaggcgc tgcctggcga tatgattttt
     9781 ttcgatcagg gcgatgccca gcacttaatg gtctggatgg ggcgttacgt catctaccac
     9841 accggaagcg ccacgaaaac tgacaacgga atgcgcgcag tcagtctgca acaacttatg
     9901 acatggaagg acacccgatg gatacccaac gattccaatc ccaatttcat tggcatttat
     9961 cgtttaaatt ttctggcgcg atagcagcgt gcttgtccct gtctcttgtg ggaacagggc
    10021 ttgctaatgc tgatgattcg cttccttcca gtaactatgc gccgcccgcc gggggaacat
    10081 tctttttgct tgctgacagc agttttagca gcagtgaaga gtcgaaagtg cgactggaag
    10141 cgccggggcg tgattatcgg cgctatcaga tggaagagta cggcggcgtg gacgttcgcc
    10201 tgtatcgtat tcctgacccg atggcatttt tgcgccagca gaaaaacctg catcgcattg
    10261 tggtgcaacc gcaatatctg ggcgacgggc tgaacaatac gctgacctgg ctgtgggata
    10321 actggtacgg caaatctcgc cgcgtgatgc agcgtacttt ctcttctcag tcacggcaga
    10381 atgtgactca ggcattaccc gaattacagc tcggcaatgc cattattaaa ccttcccgtt
    10441 atgtacagaa caaccagttt tccccgctga aaaaatatcc cctggtggaa cagttccgtt
    10501 atccactatg gcaggctaaa ccggtcgagc cgcagcaagg ggtaaaactg gaaggcgcat
    10561 ccagcaattt catctcgccg cagccgggta acatttatat tcctctcggc caacaagagc
    10621 cgggactgta cctcgtcgag gcgatggttg gtgggtatcg ggcgacgacg gtggtgtttg
    10681 tttccgatac cgtggcgctt agcaaagtgt caggcaaaga gcttctggtg tggaccgcgg
    10741 gtaaaaaaca gggtgaagcg aagcccggct cagagatctt gtggactgac ggtcttggcg
    10801 tgatgacccg cggtgtgacc gatgacagcg gtaccttgca gttacaacat atatcgccag
    10861 aacgttcata cattctgggt aaggatgctg aaggcggcgt ttttgtctcc gagaacttct
    10921 tctacgaaag cgaaatctac aacacccgct tgtatatttt taccgatcgc ccgctatatc
    10981 gcgcaggcga tcgtgtcgat gttaaagtga tgggccgcga gttccacgat ccgttgcatt
    11041 catcccccat cgtcagcgcc ccggcgaagc tttcggtgct ggacgctaac ggcagtctgt
    11101 tgcaaaccgt cgatgtcacg ctggatgcgc gcaatggcgg gcagggaagt ttccgcctgc
    11161 cagaaaatgc cgttgccgga ggttatgagt tacgtcttgc ttaccgcaat caggtctata
    11221 gcagcagttt tcgcgtggca aactacatca agccacattt cgagattggt ttagctctcg
    11281 acaaaaaaga gttcaaaact ggcgaagcgg tcagcggcaa actgcaactt ctctacccgg
    11341 atggcgagcc ggtaaaaaat gcccgcgtgc agttaagttt gcgcgctcag caattatcaa
    11401 tggtcggtaa cgatttgcgt tatgccggac gtttccccgt gtcgctggaa ggcagcgaaa
    11461 cggtgtccga cgccagcggt catgtggcgt taaatctccc cgccgccgat aaaccgagcc
    11521 gctatttgtt aaccgtctcc gccagtgacg gcgcggcgta tcgcgtcacc accaccaaag
    11581 agatcctcat tgaacgcggc ctggcgcatt actctttaag taccgccgca caatacagta
    11641 atagcggcga gtcggttgtg ttccgttatg ccgcgctgga atcttcaaaa caggttcctg
    11701 ttacgtatga atggttgcgt ctcgaagacc gcacgagcca tagcggagat ctaccgtcag
    11761 gcggcaaatc ctttaccgtc aatttcgata aacctggcaa ctacaatctg acgttacgcg
    11821 ataaagacgg cttaattctc gccgggttaa gccatgccgt cagcggtaag ggcagtatgt
    11881 cgcatactgg tacggtagat atcgtggcag ataaaacgct gtaccagcct ggcgaaaccg
    11941 cgaagatgct gattaccttc ccggagccaa ttgatgaagc attattgacg ctggaacgcg
    12001 atcgcgttga acagcagtcg ctgctatcgc atccggcaaa ctggctgacg ttacaacgtt
    12061 taaacgatac tcaatatgaa gcccgtgttc cagtgagcaa ttcctttgcg cctaacatca
    12121 ctttttcggt gctgtatacc cgtaacggtc agtacagttt tcagaacgcc gggatcaaag
    12181 ttgccgttcc ccagctggat atccgggtga aaacggacaa aacccattac cagcctggtg
    12241 aactggtcaa tgtcgaatta acctcgtcgc tgaaaggtaa acctgtttct gcgcagctaa
    12301 cggtaggcgt ggtcgatgaa atgatctacg cgctgcaacc agaaatcgcg ccgaatatcg
    12361 gcaaattttt ctatccgctg gggcgtaaca atgtgcgtac cagctccagt ctgtcgttta
    12421 tcagctacga ccaggcactc tccagcgagc cggttgcgcc tggcgcaact aaccgcagcg
    12481 agcggcgagt aaaaatgctt gaacgtccac ggcgtgaaga ggtggatacc gcggcatgga
    12541 tgccgtcact cacaaccgat aaacaaggca aagcgtattt cacgttcctg atgcctgatt
    12601 cgttaacccg ctggcgtatc accgcgcgtg ggatgaacgg cgacgggctg gtcgggcagg
    12661 ggcgtgctta tctgcgttcg gaaaaaaatc tctacatgaa gtggagtatg ccaacggtgt
    12721 atcgcgtggg cgacaaaccg tcggcaggac tgtttatctt cagtcagcag gataacgaac
    12781 cggtggcgct ggtgactaaa tttgcaggcg ctgagatgcg ccagacgctg acgctgcaca
    12841 aaggggcgaa ttatatttcg ctggcgcaga acattcagca atctggcttg ttaagcgcag
    12901 aactgcaaca aaatgggcaa gtgcaggaca gcattagcac aaaactgtct tttgtggata
    12961 acagctggcc cgttgaacag cagaaaaatg tcatgctcgg cggtggcgat aacgcgctga
    13021 tgttgcccga gcaggcgagc aatatccggc tacaaagtag tgaaacgccg caggagattt
    13081 tccgcaacaa tcttgatgcg ttagtcgatg aaccgtgggg ggggtgatca acaccggtag
    13141 ccgtctgatc ccgctcagtc tcgcctggcg ttcgcttgcc gatcatcaaa gtgccgccgc
    13201 taacgacatt cgtcagatga ttcaggataa ccgtctgcgg ctgatgcaac tggcggggcc
    13261 cggagcgcgc tttacctggt ggggtgaaga tggcaatggt gacgccttcc ttacggcatg
    13321 ggcatggtac gccgactggc aggccagcca ggcgctcggc gtaacgcaac aaccggaata
    13381 ctggcagcat atgctcgaca gttatgccga gcaggcagat aacatgccgt tattgcatcg
    13441 ggcgctggtg ctggcgtggg cacaggagat gaatctgccg tgcaaaacgt tgttgaaagg
    13501 gttggatgaa gctatcgccc ggcgcggaac taaaactgaa gatttctctg aggaagacac
    13561 ccgcgatatc aatgacagcc tgatcctcga tacaccggaa tctccactgg cagatgcggt
    13621 ggcaaacgtc ttaaccatga cgttgctgaa aaaagcgcag ttgaagtcca cggtgatgcc
    13681 acaggttcag caatatgcgt gggataaagc ggtaaacagc aatcagccgc tggcacacac
    13741 ggttgtgctg ctcaatagcg ggggcgacgc tacccaggcg gctgctattt taagtggttt
    13801 gaccgctgag caatctacta ttgagcgcgc gctggccatg aactggctgg cgaaatatat
    13861 ggcgacaatg ccttcggttg tgttgcctgc gcctgcgggc gcatgggcca aacataagtt
    13921 aactggaggg ggcgaatact ggcgttgggt tggtcagggc gtgccggaca ttctctcttt
    13981 tggtgacgaa ttatcgccgc aaaatgtgca ggtccgctgg cgtgaagcgg caaaaacggc
    14041 tcaacaaagt aacattccgg tgaccgttga acgccaattg tatcggctta tccctggtga
    14101 agaagagatg agctttactc tgcaaccggt gaccagcaat gagattgaca gcgatgcgct
    14161 gtatctcgat gaaattacgc ttaccagcga gcaggatgca gttctgcgct atggtcaggt
    14221 ggaagtaccg ctcccgccgg gagccgacgt tgagcgcaca acatggggca tttcagtcaa
    14281 taaacccaac gctggaaaac agcaggggca attgctggaa aaagcgcgaa atgaaatggg
    14341 cgaactggcc tatatggtgc cggtgaaaga actgacggga acggtcactt tccgccattt
    14401 gctgcgcttc tcgcaaaaag ggcaattcgt tctgcctcct gctcgttatg tgcgttccta
    14461 tgcacctgca cagcaaagtg ttgcggcagg gagcgaatgg accgggatgc aggtgaaata
    14521 agtgaactgg cgcagaatcg tctggctatt ggcgttggtt actctgccaa cgcttgcgga
    14581 ggagacgccg ttacaactgg cgctgcgcgg tgcgcagcac gatcaactgt atcagctgtc
    14641 gtcatcaggc gtgaccaaag tctcagcatt acctgacacg ctcaccacgc cgttagggag
    14701 tttatggaaa ctgtatgttt acgcctggct ggaggatacc catcaaccgg agcagcccta
    14761 ccagtgtcgc ggaaactccc cggaagaggt ttattgttgt caggctgggg agagcataac
    14821 gcgcgatacc gcgctggtac gttcctgtgg gctctatttt gcgccgcagc ggttgcatat
    14881 tggggcagat gtgtgggggc aatactggca acagcgtcag gcacctgcgt ggttagcgtc
    14941 tctttctacg ctgaagccag aaacctcggt aacggttaaa tcgctgcttg attcattagc
    15001 gacgttacct gcgcaaaaca aagctcagga agtcttactg gatgtggtgc tggacgaggc
    15061 gaaaatcggt gtcgcatcta tgctcggtag tcgggtacgg gtgaaaacct ggagctggtt
    15121 tgccgatgat aaacaggaaa tccgtcaggg tggttttgct ggctggttaa ccgatggtac
    15181 accgctgtgg gtgactggca gtggcaccag caaaacggta ctgacccgtt atgcgacggt
    15241 gcttaatcgg gttttgccag tgccgacgca agttgccagt gggcaatgtg tagaggtgga
    15301 gctatttgcg cgctagccgc tgaagaaaat cacagcggag aagagtacga cggcagtaaa
    15361 acccggcgta cttaatgggc gctatcgtgt gacgtttgcc aatggtaacc acattacgtt
    15421 tgtcagccac ggtgagacga ccttactgac cgaaaagggc aaactgaaat tgcagtcgca
    15481 tctcgatcgt gaagagtacg ttgccagagt tctggatcgc gaggcgaaaa gtacgccacc
    15541 tgaggcagcg aaagccatga ctgtggcgat acgcactttt ttacagcaaa acgccaatcg
    15601 ggaaggcgat tgcctgacca tccccgacag cagcgccacg cagcgtgttt ctgcttcgcc
    15661 agctaccacg ggggcgcgaa cgatgaccgt ctggacgcag gatctcatct atgcgggcga
    15721 tccggttcat tatcacggca gtcgcgctac cgaagggacg ctttcctggc ggcaagccat
    15781 ggcccaggcg gggaagggag agcgttacga ccagatactc gcgtttgctt atcccgacaa
    15841 cagccttagc cgctggggag cgccacgcac aacctgtcag ttattaccca aagcgaaagc
    15901 ctggctggcg aaaaaaatgc cgcagtggcg gcgtatatta caaggcgaga cggggtacaa
    15961 cgaaccagac gtgtttgcgg tctgtcgttt agtctcaggt ttcccctata ccgatcggca
    16021 gcagaaacgg ctgtttattc gtaacttctt tacgcttcag gatcggctgg atttaaccca
    16081 tgagtatctg cacctggcct ttgatggtta tcccaccggg cttgatgaga actatatcga
    16141 aacgctgacc cgacaattat taatggacta atgctatgcg aaagattttt cttccgttgt
    16201 tactggtggc gctttcgcct gtcgctcaca gtgaaggtgt gcaggaagtc gagattgacg
    16261 caccgctctc tggctggcat ccggcagagg gcgaagatgc cagtttttca caatcgatca
    16321 attacccggc gtcgtccgtc aacatggcag atgatcaaaa tatctcagcg cagatccgcg
    16381 gcaaaataaa gaattatgct gcggcgggta aagttcagca gggccggctg gtggtcaacg
    16441 gtgccagtat gccgcagaga attgaatccg atagttcatt tgcacgccct tatattttca
    16501 ctgaaggcag caacagcgtg caggtcatca gcccggatgg gcaaagccga caaaaaatgc
    16561 agttttactc aacgccgggt acaggaacga ttcgtgcacg tttacggctg gttctctcgt
    16621 gggatacgga caataccgac ctcgatcttc acgttgtcac gcccgatggc gaacacgcct
    16681 ggtacggtaa caccgtgctg aaaaacagtg gtgcattgga tatggatgtc acgacggggt
    16741 acggacccga gattttcgcc atgccagcac cagttcacgg gcgctatcag gtgtatatca
    16801 actactatgg tgggcgcagc gaaacggaat taaccaccgc ccaactgacg ctgatcaccg
    16861 atgaaggatc ggtcaatgag aaacaggaaa catttattgt accgatgcgt aatgctggcg
    16921 agctgacgct ggtgaaaagt tttgactggt gatccatccc ttcacacaac ctggctcttt
    16981 cttttttcag ataagactgc tgggcgatct gccgacgcca ggatataacc attaacaagc
    17041 gtcggcagat tggtcatttc ttatttttcg tactgagaac tgtccattta ctccggcttt
    17101 cctggttaag gcaatggttt ctaagcgata cgcattattg caatggtgac agaacattgc
    17161 ggtaacacgc ttttaccgct accttaacca cactccatcg gtcacctgag gcggagcttc
    17221 gcccctttga aataccttgc ttcttttcgt acaaccctga aagcctcgcg ctacatgttc
    17281 agagcattgg cgttagtgct ctggctgttg attgcttttt catccgtttt ttacatcgtt
    17341 aatgcgttac atcagcgaga atcggaaatt cgtcaggaat ttaatctgag ttccgatcag
    17401 gctcagcgct ttattcaacg cacctctgat gtgatgaaag agctgaagta catcgccgaa
    17461 aatcgcttat cggcagaaaa cggtgtgctt tccccgcgtg gacgagaaac gcaggcggat
    17521 gtgcctgcgt ttgaaccgct gtttgccgac tccgattgtt cagcaatgag taacacctgg
    17581 cgaggttctc tggagtcatt ggcgtggttt atgcgctact ggcgcgataa tttttctgcg
    17641 gcttacgatc tcaaccgggt atttttaatc ggcagcgata acctctgcat ggccaatttc
    17701 ggtctgcgtg atatgccagt ggaacgcgat accgcgttga aagctttgca tgaacgcatc
    17761 aataaatatc gaaatgcacc acaagatgat agcggcagta acctctactg gatcagcgaa
    17821 ggtccgcgcc ctggcgtcgg gtatttttac gcgttgacgc cagtttatct ggcgaaccgg
    17881 ttgcaggcgc ttttgggtgt cgagcagacc atccggatgg agaacttttt cttaccgggt
    17941 acgttgccga tgggggttac cattcttgat gaaaatggtc ataccctgat ttcgcttacc
    18001 gggccagaaa gcaaaattaa ggccgatcct cgctggatgc aggaacgctc ctggtttggc
    18061 tatacggaag ggttccggga gctggtgctg aagaaaaatc tgccaccctc atcgctcagc
    18121 atcgtgtatt cggtgccggt tgataaggtg ctggaacgca ttcgcatgtt gatccttaac
    18181 gcaattttgc tgaatgtgct tgccggagct gcattgttta ctctcgcgcg gatgtacgag
    18241 cgacgtattt tcattccggc ggaaagcgac gccctgcgac tggaagaaca tgagcagttc
    18301 aatcgcaaga ttgtcgcctc cgcgccagtg ggtatctgca ttttgcgtac cgctgatggc
    18361 gtcaatattt taagtaacga actggcgcat acctatctca atatgcttac gcatgaggac
    18421 cgccaacgac tgacacaaat tatctgtggg cagcaggtca attttgttga tgtcctgacc
    18481 agcaacaata ccaatctgca aatcagcttc gtccattcgc gctatcgtaa tgaaaacgtg
    18541 gccatttgtg tgctggtgga tgtttcttcg cgcgtgaaga tggaagagtc gttgcaggag
    18601 atggcacaag cagcggaaca ggcgagccag tcaaaatcga tgttccttgc caccgtcagt
    18661 catgagctgc gaacgccgct gtatggcatt atcggtaacc tggatctgtt gcaaaccaaa
    18721 gagttaccga aaggcgtcga tcgtctggtg acggcaatga acaactcttc cagcctgttg
    18781 ttgaaaatta tcagcgatat tctcgatttc tcgaagattg aatcggaaca gttgatgatc
    18841 gaaccgcgtg agttttcacc gcgtgaagtg atgaaccaca tcaccgccaa ctatttaccg
    18901 ctggtggtac gcaagcagtt aggcttgtac tgctttattg aaccggatgt gccagtggcc
    18961 ttaaatggcg acccgatgcg tttacagcag gtcatctcca acctgttgag taacgccata
    19021 aaattcaccg ataccggctg tatagttttg catgtccgcg ctgatggcga ttatctctct
    19081 atccgtgttc gcgataccgg cgtggggatt ccggcgaaag aagtggtgcg cttgtttgat
    19141 cccttcttcc aggtcggaac gggcgtacag cgaaacttcc aggggaccgg tctgggtctg
    19201 gcgatttgtg aaaaactgat cagcatgatg gacggcgata tctcggtaga ttcagaaccg
    19261 ggaatgggca gccagtttac cgtgcgtatt ccgttgtacg gcgctcagta cccgcagaaa
    19321 aaaggcgtgg aagggttgag tggtaaacgc tgctggctgg cggtccgcaa tgcgtcgctc
    19381 tgtcagttcc tggaaaccag tttgcagcgc agcggcatcg tcgtaacaac atacgaaggg
    19441 caggaaccga ctcccgaaga tgtgttaatc actgacgagg tagtgagtaa aaaatggcag
    19501 ggcagagcgg tagtgacctt ctgtcgtcga catattggta ttccgctgga gaaagcgcca
    19561 ggggagtggg tacacagtgt ggctgctccg catgagctac cggcattgtt ggcgcgtatt
    19621 tatttgatcg agatggagag cgacgatcct gctaacgctc tgccgtcgac ggacaaagcg
    19681 gtcagcgata atgacgatat gatgattctg gtcgtggatg atcatccgat taaccggcgt
    19741 ttgctggcag atcagttggg atcgttgggc tatcaatgta aaaccgcgaa tgatggcgtc
    19801 gatgcgctta atgtacttag caagaatcat attgatatcg tgcttagcga cgtcaacatg
    19861 ccaaatatgg atggttaccg cttgacacaa cgcattcgtc agttgggact gacgttgccg
    19921 gtaatcggag taacggctaa tgcgttggct gaagagaagc agcggtgtct ggagtccggt
    19981 atggacagct gcctgtcgaa gccagtaacg ctggatgtta taaaacagac gctgacggta
    20041 tatgccgaga gggtcaggaa atcgcgggaa tcgtaggacg gataaggcgt ttacgccgca
    20101 tctggcattc agtgcaaatg ccagatgcga cgctgacgcg tcttatctgg cctactttaa
    20161 tgtaatctgt tgaaataatg ggaatcgtag gacggataag gcgtttacgc cgcatccggc
    20221 aatcggtgca aatgccagat aagacactaa cgcgtcttat ctggcctaca gatgattagt
    20281 ctttatctgc cggacttaac gttactgaag agagataatt cagcagggcg atatcgttct
    20341 cgacacccag cttcatcatc gcagatttct tctggctact gatggtttta atactgcggt
    20401 tcagcttttt agcgatctcg gtcaccagga agccttccgc aaacaggcgc agaacttcac
    20461 tctcttttgg cgagagacgt ttgtcaccgt aacctccagc actgattttt tccaacaggc
    20521 gagaaacgct ttccggggta aatttctttc ctttttgcag cgctgcgagg gctttcggca
    20581 gatcggtcgg tgcaccttgt ttcagcacga tcccttcgat atccagatcc aataccgcac
    20641 taagaatcgc cgggttgttg ttcatggtca gaacaataat cgacaggctt gggaaatggc
    20701 gcttgatgta cttaattaag gtaatgccat cgccgtactt atcgccaggc atggagagat
    20761 cggtaatcaa cacatgcgca tccagtttcg gcaggttgtt gatcagtgct gtagagtctt
    20821 caaattcgcc gacaacattc acccactcaa tttgctcaag tgatttgcga ataccgaaca
    20881 agactatcgg atggtcatcg gcaataatta cgttcatatt gttcatgtaa taggctacct
    20941 tgctacagca agctcttgac ataactgtca atgtcgctga tgtatttttc tattcctgga
    21001 acatccttct cacgaatcag atgttccagc gtttcacata actgcttgcc gggtaccaga
    21061 tttagcatgg caaatacgcc tttaagacga tgagccgttt gggctaacgc agcaaagtca
    21121 ctggttgctg cttcagtata cagcctctta acatcatccg gtactgtgtc tacaaagagc
    21181 gcataatagc cgctggcatg gagttgcgca ttttcatctc cgcccagagg cgattctgtc
    21241 acctcttcct gcgccagttg cacttcaatt aattgtaaga ccgcttcctg catagcgttg
    21301 ctcatattga agttgacgca caattgacca ggcccaattt cccgtacgcc agactcatca
    21361 tcgcttaaaa gcaagccaga ggcagtaaga ttagacggat tatccgttaa aaagatatca
    21421 taatcttgac taattaatct ttcatcgggt gtgatacagg ttgcacccca attttctaac
    21481 tggcgagtga caatattccg aatttctgcc gaagtaacat ccaccattac gcagacatca
    21541 tccagtaaac gctcttcttc ctcttcaact tccgggtcag ctgcgagcat tttgatatgc
    21601 acagagtagc gtgtaccaag cccatcccgc gttttgatgt ttaaatgacc gcccagttta
    21661 cgtgccagtt gatcgctcag ccagaatgcc agcgggtccg ccttgccata gcgatcgttt
    21721 tgggtctggt tgataaacgg gaagtgcaaa ttatccattt catgaatgct tacgccttct
    21781 ccggtgtcca gaatgcggaa cgtcaggcgg tcttcggagg actcatcctg atcaacctca
    21841 agggtgattt ttcccaattg cgttgaggtc acggcatatt gcatcagtag cagcaaaata
    21901 cgtcgtaatg catcgcgatc gccgcggcgc atatcgtgtg ctttcagatg attgttaatc
    21961 agcagttgca gacctttacg cttgatggca ggcaacactg aaggcacaac ttcatcaatt
    22021 aaatcctgca cggagaacag caccgtctca cttttccagc tatcgtccgc aagcatgttc
    22081 gctaactgta tttcatcgac caatcgcacc agcacatctg cctgattcgc cagttgtttg
    22141 ctttccgggg cgttgagttt agccgcgctc tccgccaggg actgtgcggg ttctttcagc
    22201 gcatcgccaa tgtttttcat aaaggtcatc cgcccctgct ggtttttctc atacagacgc
    22261 tgcgcctgct tgagtttctt gtttaccagc acttcgcgat cctgatcgcg aataatgaaa
    22321 atttgtgtgc gcggcgcaac ctggctgcgg aacatgcgga tctcatacag ctcgttattg
    22381 atcgtcgcct gaataatccc ctgatgctgt tccgccatgg tggtgatgtt ttgcagattc
    22441 aaatgcggca gcaaatgatc ggcaattttg ttacttatga cagtgcggtt cgattcctga
    22501 tcgtgaacca gcaggccgag cggcagcagt gagactatct cttcattgat tgcccgtaaa
    22561 atgcgcaatt cgttattgac cgccgtgttg ggtagacttt ctgtactgcg actggagaaa
    22621 tggcggaatg tggtatagcc aaataacgcc agcgccagca aaccgatgtt cagcagcagt
    22681 ggcagcagaa tgttttgcaa cgtatccagc aataaggtgc cataaggaac ctgccagacc
    22741 aggcgcatat cggtagagtt gagtgccgag gagatttcaa tcttcgtact gttaaagtgg
    22801 atactgacgc tatccgttcc ttctttctca ttatcattgt ttcccgttgc cgtcgcgtct
    22861 ggctcaaggc ggaaactgtc cagcggcata cccggtggga tcaaatcatt aatcggcaga
    22921 tcaaaagcca cgaccgttgc cagatgtccc ggctggttga acgtggtacg caaggtaaag
    22981 taatgaccgt tctgccaggc caggcggcgc atgttagaaa aactttcgcg ttcatcgagg
    23041 gcgttggcct gttgcaacat ctctgcacga cgtgaatcaa caatgtcact gacggtcgat
    23101 tctttaaatc cggaggtgag atctttgagg ggcagggttg agatcagcac cagactgtta
    23161 tcctgaccat tcaggtaata cattgaccac ggtacatttt ctgcgcccca caatgtgtcc
    23221 agataggtgg acatccgttg agtcatctca agcgttgagt tgtcgtgaga gccaaagatt
    23281 aacgcttccg ttttgcggcg cggtttttcc agatagtaga catcctgttt caggcgcgtc
    23341 tcttgtaaac cttcgccgga ggagggggag gtcgtcgcgg caatgttgtc gtagatctgc
    23401 caggtcacgt aacgccaggt atcgacgcgc ttttggatag cgtgggtaat gtcgacaatt
    23461 tggtaacttt tatctttcag ccaggcgtta acggcgcttt gtaccattac acccatcgtc
    23521 accagtaaca caatgatcaa cagtaaaaag aagcgggtaa tgctccccgg taggagtgaa
    23581 aagcgggtcg tggccgttgt ctctttctga cgcattcgtg tttatgacct gttaaaactt
    23641 cgcgaagtga tagggcagta taaagggtac agtgtgaaat tccagactct tacctgattg
    23701 atgattctct attctcccgt gatatttatc atcctgcatt gggggaaata gatgtacaag
    23761 cgccattttg ctgtacaaat aacctacaaa aagcccaacg aaaataattg caaataaaca
    23821 aagattgctg gaaattatgc tgatgttaat tatttgtgaa atagttaaca agcgttatag
    23881 tttttctgtg gtagcacaga ataatgaaaa gtgtgtaaag aagggtaaaa aaaaccggat
    23941 gcgaggcatc cggttgaaat aggggtaaac agacattcag aaatgaatga cggtaataaa
    24001 taaagttaat gatgatagcg gtagttattc tagttgcgag tgaaggtttt g
//
LOCUS       DABSYT020000067        23530 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.067, whole genome shotgun sequence.
ACCESSION   DABSYT020000067 DABSYT020000000
VERSION     DABSYT020000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.067"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1001)
                     /locus_tag="ID725_RS15845"
                     /old_locus_tag="pgaptmp_003169"
     CDS             complement(<1..1001)
                     /locus_tag="ID725_RS15845"
                     /old_locus_tag="pgaptmp_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HGV7851707.1"
                     /translation="MSMVEWISHEKKDSLILFVHGLNGGLETWNFNKETSFPKLLAED
                     EDIGNVFDIACFNYFTKFTQTYAKTSGLWARIFSKKNKLERNLPTDEIAELLYTEIRV
                     TLSDYSRIIIIAHSMGGVISKNLILKKVEHEEQSNIIGFISLAVPHFGAKLANITSMV
                     SSNAQLVDLGLLSEATDTLNRRWINCSKKLPITRYVYGSHDTIVDKKSALPMDSERNN
                     SIAVNEGHSSICKPENSSSTVFVAVKQFIQQINLEAPKKICVERFSDEKQYDNEYFVL
                     KMIVADIHQDIAMHAKEYYYNAELARNIFTSDYDRKLLGHLYSKIREIYQEEYEQYIA
                     "
     gene            complement(1091..3373)
                     /locus_tag="ID725_RS15850"
                     /old_locus_tag="pgaptmp_003170"
     CDS             complement(1091..3373)
                     /locus_tag="ID725_RS15850"
                     /old_locus_tag="pgaptmp_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGV7851708.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDHIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYGELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKIYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVRKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGKPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPTEHAAAVLNLIDEGILSWTEPGIMKVLRDLLSNELKCSNRS
                     QQSFTPFNGRSYFPAPSARLTRQERKTIPKIKVLLAQSDIQASYWELEALARGAVLDF
                     GHKRFKFDNDIDFFDRQREW"
     gene            complement(3363..3638)
                     /locus_tag="ID725_RS15855"
                     /old_locus_tag="pgaptmp_003171"
     CDS             complement(3363..3638)
                     /locus_tag="ID725_RS15855"
                     /old_locus_tag="pgaptmp_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HGV7851709.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(3635..3859)
                     /locus_tag="ID725_RS15860"
                     /old_locus_tag="pgaptmp_003172"
     CDS             complement(3635..3859)
                     /locus_tag="ID725_RS15860"
                     /old_locus_tag="pgaptmp_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HGV7851710.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKRYRRTA"
     gene            complement(3862..4161)
                     /locus_tag="ID725_RS15865"
                     /old_locus_tag="pgaptmp_003173"
     CDS             complement(3862..4161)
                     /locus_tag="ID725_RS15865"
                     /old_locus_tag="pgaptmp_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HGV7851711.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(4161..4385)
                     /locus_tag="ID725_RS15870"
                     /old_locus_tag="pgaptmp_003174"
     CDS             complement(4161..4385)
                     /locus_tag="ID725_RS15870"
                     /old_locus_tag="pgaptmp_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HGV7851712.1"
                     /translation="MHTVSENKCGKYALLLQQARAEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(4449..4949)
                     /locus_tag="ID725_RS15875"
                     /old_locus_tag="pgaptmp_003175"
     CDS             complement(4449..4949)
                     /locus_tag="ID725_RS15875"
                     /old_locus_tag="pgaptmp_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HGV7851713.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4946..5116)
                     /locus_tag="ID725_RS15880"
                     /old_locus_tag="pgaptmp_003176"
     CDS             complement(4946..5116)
                     /locus_tag="ID725_RS15880"
                     /old_locus_tag="pgaptmp_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851714.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(5127..5402)
                     /locus_tag="ID725_RS15885"
                     /old_locus_tag="pgaptmp_003177"
     CDS             complement(5127..5402)
                     /locus_tag="ID725_RS15885"
                     /old_locus_tag="pgaptmp_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HGV7851715.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            5524..5823
                     /locus_tag="ID725_RS15890"
                     /old_locus_tag="pgaptmp_003178"
     CDS             5524..5823
                     /locus_tag="ID725_RS15890"
                     /old_locus_tag="pgaptmp_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7851716.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5939..6952
                     /locus_tag="ID725_RS15895"
                     /old_locus_tag="pgaptmp_003179"
     CDS             5939..6952
                     /locus_tag="ID725_RS15895"
                     /old_locus_tag="pgaptmp_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGV7851717.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(7217..7534)
                     /gene="yegR"
                     /locus_tag="ID725_RS15900"
                     /old_locus_tag="pgaptmp_003180"
     CDS             complement(7217..7534)
                     /gene="yegR"
                     /locus_tag="ID725_RS15900"
                     /old_locus_tag="pgaptmp_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGV7851718.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            7940..8839
                     /gene="yegS"
                     /locus_tag="ID725_RS15905"
                     /old_locus_tag="pgaptmp_003181"
     CDS             7940..8839
                     /gene="yegS"
                     /locus_tag="ID725_RS15905"
                     /old_locus_tag="pgaptmp_003181"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGV7851719.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(8921..9694)
                     /locus_tag="ID725_RS15910"
                     /old_locus_tag="pgaptmp_003182"
     CDS             complement(8921..9694)
                     /locus_tag="ID725_RS15910"
                     /old_locus_tag="pgaptmp_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGV7851720.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(9800..10840)
                     /gene="gatD"
                     /locus_tag="ID725_RS15915"
                     /old_locus_tag="pgaptmp_003183"
     CDS             complement(9800..10840)
                     /gene="gatD"
                     /locus_tag="ID725_RS15915"
                     /old_locus_tag="pgaptmp_003183"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGV7851721.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(10888..12243)
                     /locus_tag="ID725_RS15920"
                     /old_locus_tag="pgaptmp_003184"
     CDS             complement(10888..12243)
                     /locus_tag="ID725_RS15920"
                     /old_locus_tag="pgaptmp_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGV7851722.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(12247..12531)
                     /gene="gatB"
                     /locus_tag="ID725_RS15925"
                     /old_locus_tag="pgaptmp_003185"
     CDS             complement(12247..12531)
                     /gene="gatB"
                     /locus_tag="ID725_RS15925"
                     /old_locus_tag="pgaptmp_003185"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGV7851723.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(12562..13014)
                     /gene="gatA"
                     /locus_tag="ID725_RS15930"
                     /old_locus_tag="pgaptmp_003186"
     CDS             complement(12562..13014)
                     /gene="gatA"
                     /locus_tag="ID725_RS15930"
                     /old_locus_tag="pgaptmp_003186"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGV7851724.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(13024..14286)
                     /gene="gatZ"
                     /locus_tag="ID725_RS15935"
                     /old_locus_tag="pgaptmp_003187"
     CDS             complement(13024..14286)
                     /gene="gatZ"
                     /locus_tag="ID725_RS15935"
                     /old_locus_tag="pgaptmp_003187"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HGV7851725.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(14315..15169)
                     /gene="gatY"
                     /locus_tag="ID725_RS15940"
                     /old_locus_tag="pgaptmp_003188"
     CDS             complement(14315..15169)
                     /gene="gatY"
                     /locus_tag="ID725_RS15940"
                     /old_locus_tag="pgaptmp_003188"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGV7851726.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(15468..16520)
                     /gene="fbaB"
                     /locus_tag="ID725_RS15945"
                     /old_locus_tag="pgaptmp_003189"
     CDS             complement(15468..16520)
                     /gene="fbaB"
                     /locus_tag="ID725_RS15945"
                     /old_locus_tag="pgaptmp_003189"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGV7851727.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            16777..18054
                     /locus_tag="ID725_RS15950"
                     /old_locus_tag="pgaptmp_003190"
     CDS             16777..18054
                     /locus_tag="ID725_RS15950"
                     /old_locus_tag="pgaptmp_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGV7851728.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18051..19055
                     /locus_tag="ID725_RS15955"
                     /old_locus_tag="pgaptmp_003191"
     CDS             18051..19055
                     /locus_tag="ID725_RS15955"
                     /old_locus_tag="pgaptmp_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGV7851729.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            19052..20017
                     /locus_tag="ID725_RS15960"
                     /old_locus_tag="pgaptmp_003192"
     CDS             19052..20017
                     /locus_tag="ID725_RS15960"
                     /old_locus_tag="pgaptmp_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGV7851730.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(19991..20737)
                     /locus_tag="ID725_RS15965"
                     /old_locus_tag="pgaptmp_003193"
     CDS             complement(19991..20737)
                     /locus_tag="ID725_RS15965"
                     /old_locus_tag="pgaptmp_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGV7851731.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(20789..21607)
                     /locus_tag="ID725_RS15970"
                     /old_locus_tag="pgaptmp_003194"
     CDS             complement(20789..21607)
                     /locus_tag="ID725_RS15970"
                     /old_locus_tag="pgaptmp_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGV7851732.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(21672..22472)
                     /gene="thiD"
                     /locus_tag="ID725_RS15975"
                     /old_locus_tag="pgaptmp_003195"
     CDS             complement(21672..22472)
                     /gene="thiD"
                     /locus_tag="ID725_RS15975"
                     /old_locus_tag="pgaptmp_003195"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGV7851733.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(22469..23257)
                     /gene="thiM"
                     /locus_tag="ID725_RS15980"
                     /old_locus_tag="pgaptmp_003196"
     CDS             complement(22469..23257)
                     /gene="thiM"
                     /locus_tag="ID725_RS15980"
                     /old_locus_tag="pgaptmp_003196"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGV7851734.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(23292..23388)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         6025 a         6016 c         5436 g         6053 t
ORIGIN      
        1 gcgatatatt gctcatactc ttcctggtaa atctccctta ttttagaata caaatgacca
       61 agtagtttcc tatcgtaatc actcgtaaaa atatttctcg caagctcagc attataataa
      121 tattcctttg catgcatagc gatatcctga tgtatatctg cgacaatcat ttttaagacg
      181 aaatactcat tatcatattg tttctcatca gaaaaacgct caacacatat cttcttgggt
      241 gcttctaaat ttatctgctg aataaattgc tttaccgcaa caaacactgt agacgagctg
      301 ttttcgggct tgcaaattga gctatgccct tcattaactg caattgagtt atttcgttca
      361 ctatccatcg gtaatgcgct ttttttatca actatagtgt catgagaacc ataaacatat
      421 ctagtgatag gaagtttttt gctacaattt atccagcgcc tattcaacgt atcggtcgct
      481 tcacttaaca atccaagatc tactaactga gcatttgaag ataccattga agttatattc
      541 gccaatttag caccaaagtg agggactgct aacgaaatga atccaattat attagattgt
      601 tcctcatgct caactttttt tagaattaga ttttttgata ttaccccccc catactatgg
      661 gcgattatta taattcgaga gtaatcactt agcgttaccc taatctcagt atacaataac
      721 tcagctattt catcagttgg taaatttctc tctaacttat ttttcttaga gaaaatacga
      781 gcccataatc ctgacgtttt agcatacgtt tgtgtaaatt tggtgaaata attgaaacat
      841 gcaatatcaa atacattgcc aatgtcttca tcttctgcta atagttttgg aaatgaagtt
      901 tctttattaa agttccatgt ttccaaacca ccatttaatc catgtacaaa tagtatcagg
      961 ctatcttttt tctcgtggct tatccattca accatgctca tatgcttttc tgccgtgtat
     1021 aaaaattaaa ctataacaaa caattcctca acaataacat attgttaccc cgtttgaccg
     1081 tttttttcaa ctaccactca cgctgtctgt caaaaaagtc gatatcgtta tcaaatttaa
     1141 aacgcttatg cccaaaatcc aaaacagctc cgcgagcgag agcttcaagc tcccaataac
     1201 tagcctgaat gtcactttgt gcaagaagaa ccttaatttt cgggatagtc tttctctcct
     1261 gccttgtcaa ccgggcggat ggggcaggaa agtagcttcg accattgaaa ggggtaaagc
     1321 tctgctgtga acgattactg catttcagtt cattactcaa taagtctcta agtaccttca
     1381 taatgcctgg ctcggtccaa cttagaatcc cttcatctat taaatttaac accgctgcag
     1441 catgctcagt cggtgtggga gccggtaacg aagtatcacc accggtgagt tttccacagt
     1501 tattgacagg actccgaggc gcggcgatgc cgctttttaa agtcaaaggc tcaacgaccg
     1561 gaactttcgg cacaatgcgc cagtccgtcg ttctggtgat atgaatatga cgcgcgccga
     1621 gatgcggcgc gtaaatgccg accactctct cgacttcttc ctcgtactcg ttaacgtcat
     1681 ccgacgggct acgggcgacc ctgacagtct gacaatcgcg cgggacattt gccccaccct
     1741 gcgcgctgat atacaacgca aaatcaccac tgtctgcggc agcgcgagca gcctcgacgc
     1801 gttcgtcaaa ctcatcagca atactgacgc cgcgaggcaa tttgcgtagt tcacggtaag
     1861 cccccattgt cggcagtcca accgttttaa attgcggaat gcgccacgtt gacgcccatg
     1921 cggtaacagc cgcggcagta tctttaagcg gcttaccggt atcgttatcg agctgaccat
     1981 ccagtgcata gccgtcgatg ttttttgaaa tgtatttcgc gatatatccc gcagcaccgc
     2041 cccggttaag gtgttttgcc tgaaaacggt ttcgcgcagc tcctctttcg tctccatcct
     2101 ctttgagcgc gtagcgacgc atgatttcga taatctggtt acgctggcgt ggattacaaa
     2161 aaagcatcat atgccagtgc ggcgttccgt cgtggtgtgg ctcgacgaca cgcaaaccgt
     2221 agacctgtaa atcattatcc ttgaatgccg tgcgcatcag gctccagatg cggcagagat
     2281 aacgctgcgc atcctttgga ttaaatgcct catcgttcca gccgtgatta agctgaacgg
     2341 ttttactttc gccttttctg acctgacgtg tcgggtgata ctttgacggc gcggtcagcg
     2401 tgataaacat ccccacatca ccctctgcgg cggcgtaacg ctcaataccg gcaatggtgt
     2461 tcatcagctc catccggcga atttcaggat tagaaatact gcccatcacc ttactgataa
     2521 ggtcgatgcg ctcgccggtt tccctgtttt caaggtcaca cgatttaaga aattccagat
     2581 ttgcctggcg gcgcgcacgc acatcacgaa tggcgtgttt actggcataa ggagaacggt
     2641 ctttattgac ctccccgaca gctatcagta acgcttcatg ccagcgcata cgctggcctt
     2701 taagctgatg agtccaccac tcatcgttaa acagacgggc aatggcagaa tatgcctgcc
     2761 tcgtggtcat ctgcccttta cggtatattt tccagtagag aggggaaata ttgaaagcac
     2821 gtgcagcgcc agcaacatga ccatagaggt gagcctgcgc ctcatccgta aacagcgatt
     2881 ctttttcgcc atgcgcatcc acccaggcat cactgagttc cccatacatc atgaaaagct
     2941 gcgatgagat acgggcagca aactttttca gctccttgtc attcattccc ggcaggcgcg
     3001 catagtggtc acgctctgcc agaaacagta acgacgcgtc ggtgttcatt tcatggcgct
     3061 gattcacacg ctcaatgcgc ggccataaac gacgctgaaa agtggatgtg aggaaataaa
     3121 acccgtgcac cgggctttta ttgcgccgga tgtggtcata gcgtgaagta aacagcgagc
     3181 gcaaaaagta aggcaggcgg ttaatcgtgg ataaaacacc ttgcacctga cgcatctcgt
     3241 cacgtgtaag gggtctttcg cgcccgacgg cctcgcgtgg cgcgttccat gcataagcac
     3301 cggtaaacgc cttaccggtg cctgcagcaa atgctgacgg agggacaaaa cgcccggagg
     3361 ctttaacggc catatgagcc aaaagcctct gagcaacgct tgctgagttg ctcaacctgc
     3421 gcgtttaaat cagcaaaaga ctttgcgctt ccggtcagaa tatcgtgatg catcaggccg
     3481 gaaacgagct ggcttaattt cgggtaataa ccaaccaccg ccagccattc ctgaccggcg
     3541 tttttaccgc tttccgctct ctttttctcg tggagaataa actgaaagct gtcactggta
     3601 acgacataac gttcgccaat ttcaatacga atactcatgc cgttctccgg taacgtttgt
     3661 tttttgcttc aaagactgac tgacaggaaa cacaacgcgt ggctgacggg taagccgcac
     3721 gacgggcagc aggtattggc gcgtcacact cttcgcaaac cagcgcagaa gcaccgcaat
     3781 gttttaccct tgccgcgtta atctggcgct ccagtaattc agcctgttgt tcctgaataa
     3841 aatctacgtt gtccggcatt atcagctcct tttatcgtta agtttcctgg atacatcagc
     3901 gcaataactg gcaagttctg tcgttaattt tgtcagttca tccactgagg aaatttgctt
     3961 gtggaataca gcgcgtttaa caagtaaatt gaccacatca gacaggaggt ttaattcatt
     4021 ctgataaatc gcgataacag attcagttat gtcgcgtttt tctttatcaa ggcaaagttg
     4081 aataagagac aaatcgccat ttttcataac ggcgattttt aaggcgttat tcagtaatac
     4141 aactgaatga gaacaggaca tcaaagcacc tccccgcgag acaatccgat attgtgaaat
     4201 ttttccgact cctgactgag cagctcgact atctccacgc gggataactc cgcctttgtg
     4261 atatggcgaa tcatggcgtc aagatgagaa gaaaagcgtg tcgctgcgtc ggcctgtgct
     4321 tcggctctgg cctgttgcag cagtaatgcg tatttaccgc acttgttttc agaaactgta
     4381 tgcatgactt tctccaggca aaaagaagcc ccgcacgatt aagtgcgtta aaaactctgg
     4441 ttaattattt aatgcagata ttgctctggt tttaccgacg tcagaattgt cggtgcatac
     4501 tcaaacaggc tgaataattc acgtaatgca cggaataaag catcacgcca gtaacatgat
     4561 tcttcattaa ttcgccagta tggctggttg aattcttttt cagtcaatcc ggcatgcata
     4621 aataaagtac gacgctgact gactgttaaa aaactaatat atgcatactc acttgcgcca
     4681 acctgacggc gttttgagaa tgccccacgc aattcatcaa ttgcacaaac cagccgttca
     4741 cgttcgacgt cgttcatttc ttcaaaacgc atcgttgcgt gacgctgttt taactgcgca
     4801 tggaagcaaa ccgttagccg ttcgcgttcc atcatctgat tataataatc acatgtatcc
     4861 tgccagcgag ggacggcaag atgcttgcca attatccggc gcatagctgc tggctgtttt
     4921 tcaacgagat tgagcgtcat cactgtcatt tccagaccct ccggcttttc agaaaggtca
     4981 gagccttttt taacggactc tgttttttgg tgcggataat gattccctta cgccccttac
     5041 cgtgggtgat ggtgaagtca atcgccctgg ggctttcgtt acgcaataac tgagcaatac
     5101 aacgaggctc attcatacgg ttctccttaa cgtggttcac cgagacctaa ccacatcaac
     5161 cagccgtcac gaatctcttt aggacggctt tcataagcca gttttagtcc gttattccat
     5221 gccggaaggt atacccaata ttcaccagca cgccccgata ctgactgagg gtcagtaatc
     5281 tcaataactg gtaatttccc tttctcaatc atgcccctta cagctcttgg agttttacca
     5341 atgagttttg caaactcctg ataaggcacg gcatcagtca cgcttacaag ctgtctattc
     5401 atctgctacg attctccctt agtgcttcta atggctccta atggctaatt attgcctaaa
     5461 aggataactc cagaagcaca acatttcaca ctatcagcaa gaaattacgc aatcggagta
     5521 attatgtcaa tagacgtttc ggagaagttg aagctaatcc gtgaatctga aaggttaaac
     5581 cgtaaagaat tcagtgaatt aactggtgta gcctacagct cactttcgag ctatgagagc
     5641 cggtcaaaaa acgctggagt tgaagccata atgaaggtct tacaacatcc tagatttact
     5701 aaatatactt tgtggttcat gactgatcag gtagctccag aagccgggca aattgcgccc
     5761 gctctcgcac actttgggca aaacgaaaca acgtcgcccc actccggtca aaagactggt
     5821 taacaattta tcgtgaatat attcatcaca agtgcctact attggtggct aaatttcagc
     5881 caccacgaaa aaagcgatta gtagtagcaa aaaaaagtac cactcggagg gttttctgat
     5941 ggcaatcaaa aaactcgatg atggtcgata tgaagtggac atccgcccta ctggacgtaa
     6001 cggaaaacgc atccgtagga agtttgataa gaaaagcgaa gctgtcgctt tcgaaaaata
     6061 cacgttgtac aaccaccaca ataaagaatg gctatcaaaa ccaacagaca aacgacgtct
     6121 gtcggaactg acacagatct ggtgggattt aaagggtaaa cacgaagagc atgggaaatc
     6181 taatcttgga aaaattgaaa tcttcacaaa aataacgaat gacccatgcg catttcaaat
     6241 cacgaaatcc cttatcagcc agtactgcgc cacccgaaga agtcagggta ttaaaccttc
     6301 gagtatcaat cgtgatttaa catgtattag cggcatgttt acagccctga ttgaagcgga
     6361 gttattcttt ggtgagcatc ctatcagagg gacaaagagg cttaaggagg aaaaaccaga
     6421 aacaggctat ctcacacagg aagaaatagc cttactgctt gcagcacttg acggcgacaa
     6481 taaaaagatt gcgattcttt gcctaagtac aggagcacgt tggggagaag cagctcgttt
     6541 gaaagcagaa aatatcatcc ataaccgcgt cacgtttgtt aaaacgaaaa caaacaaacc
     6601 acgcaccgtc ccgatctcag aggctgttgc caaaatgatc gcggataaca aacgaggttt
     6661 tttattccct gatgctgatt accctcgctt cagacgaaca atgaaagcaa taaaaccgga
     6721 tttgccaatg gggcaagcca cacatgcact aaggcacagc tttgccactc atttcatgat
     6781 taatggagga agtattatca cgctacaacg gatactaggt cacacgcgga ttgagcaaac
     6841 tatggtttac gctcattttg cgccagagta ccttcaggac gccatttctc ttaatccgct
     6901 aagaggtggt actgaggccg agagtgtcca cacagtgtcc acagtagagt aacgtttaag
     6961 ggctttcagt ggtaatttat gccgctcaaa cccgcattgt accgttgaaa gcccctactg
     7021 gtgacaccct aaatctccct tacacgggct tattttttat gcataagccc tatccctgat
     7081 aaccgtcttc cattgaccac atcgatagaa tctcccttca tagcacgatg cctttcacgt
     7141 aacggcatcg tgctcgcaca ggttccggct aagcacaacc agaacgcgca tgtttgacgc
     7201 ttaccaaaaa atattctcac tctccacatt tgaatgtcag acgagcgacg ccatgtaatc
     7261 ctgcaccttc tgtcttcagg tcaactatct gcattttttt gccctgagta acacagaaat
     7321 gggctgcatc attttttact atattttctg caccagatat tctgcccctg gctaaagaag
     7381 cttcggcttc ggtgtagtat tggttatcga gtttacgctg aatattactt ttatatgcaa
     7441 gaccaaattt accgatactt gtctcatcat tatgcacagc acaaccagac ataagaaaaa
     7501 cactaattaa tgatatagca gctatctttt tcatctcacc ttcccccatt aaataccaac
     7561 gacactctct agtgtttaaa tataataatg gcatgattat tataattgaa taggattata
     7621 ataaatgttc tgtaaaacat ttcctacata agtaggaatt acggacatgg aggcccttcc
     7681 gggtaactcc atggaggctt taatatatta tattgaagaa gccactgttt agttgaatat
     7741 taggtatatg ctcttttttg aaatttatcg gtggctgccg ttagtattcg ctgtccccat
     7801 tgcaagctcc tggtggtaac cactgaatcc tccatacttg aactgactat ttatcctccg
     7861 actttcatcc tgttctgact ccaccttttc ttttctgctc tacactatct atagaccaat
     7921 cataaaggca catacgatca tggcagaatt tcccgccagc ttactgattc ttaatggcaa
     7981 aagtactgac aatctaccct tgcgcgaagc aattatgctg ttgcgtgagg aaggaatgac
     8041 gatccatgtg cgggtcacct gggagaaagg cgatgccgca cgatatgtag aggaggcccg
     8101 gaagttgggc gtcgcaacgg tgattgccgg tggtggcgat ggcaccatta atgaagtttc
     8161 tacggcgttg attcagtgtg agggggatga catacccgcg ctgggaattt tgccattagg
     8221 aaccgccaat gattttgcca ccagtgtagg gattcctgag gcactggata aggcgctgaa
     8281 actggcaatt gccggtaacg ccattgcgat agatatggcg caggtcaaca aacaaacctg
     8341 ttttattaat atggcgacag gcggatttgg gacgcgtatt accacagaaa cgccggaaaa
     8401 attaaaagcc gcgctgggtg gcgtctctta catcattcat ggcttaatgc gcatggatac
     8461 tctgcaaccg gaccgttgtg aaatccgcgg tgaaaacttt cactggcaag gtgacgccct
     8521 ggtcattggt attggtaacg ggcgtcaggc cggtggcggt caacaattgt gcccgaacgc
     8581 gttaattaac gatggcttgc tgcaactgcg catttttacc ggcgatgaaa ttcttccggc
     8641 tctcgtatca accttaaaat ctgacgaaga taacccgaat attatcgaag gcgcttcgtc
     8701 gtggtttgat atacaagccc cacacgaaat cacttttaat cttgatggcg aaccgttgag
     8761 tgggcaaaac ttccatattg aaatacttcc ggcggcgttg cgttgtcgat taccaccaga
     8821 ttgtccatta ttgcgttaat caaactattc tctcttgaac cagggagaat ccgtaatgcc
     8881 ggatgcggtg taaacacctt atccggcata cagaacaata ctacaccgta attaatttta
     8941 cccgtgtctt ctgcaatgcc agtttatcca catctgacac ggcatcatcg gtaataataa
     9001 tgtcaatttt ttcgattggc agtacctgat taaaaccgcg acgattaaat ttcgatgaat
     9061 cgagaacggc aatcacttta tttgcggcgg tgaccatcgc cccgctaatc gaataacctt
     9121 cattaaaggt cgtaatacca ttaaccgcat caataccatc agcaccgaca aacattaaat
     9181 cggcattaat atcctgcaat gaacgctcgg caatggaacc atgcatcgag cgcgttttat
     9241 ggcggaccgt gccaccacag acgaccagag taatgtcttt attttcggaa agggcaaacg
     9301 ctgccgggag actgttggta atcaccgtga tatttttggc ggtcattaat ccttcggcga
     9361 taagcattgt cgtacttccg ctgtcgagaa taacggtcat cccttcgtgg atcattttga
     9421 ctgccgcctg cgctattctg ttttttggcg cgctggcaag ctgaaatcgc tctttaaacc
     9481 cgacttcctg ggtctcggtt tcactattac cagacattat tttcgccgca ccgccatgaa
     9541 agcgcgtaac cacgcctttt tgttcgagaa agcgcaaatc ggcacggatt gtcgcttccg
     9601 aggcagcaaa tactcccgcc agatcctgaa caagcacggt tccctgttca ttcactaatt
     9661 ggatgatctt attccttcgc tcgaatgaat tcatcgtcat ttacccatat tcaattgtgg
     9721 ctagtgtaaa cgaaggggga ccattggtga atgatctgtt ttgcacaata ccgggccagc
     9781 atcacgctgg cccgcggttt cagggaatga gcaacacttt gcccggcata gcattacgag
     9841 cgatgtcacg caccgcctgg gcgaagcttt caaagcttcc acggtgagcg attaatggct
     9901 ccaggcttaa cttacgttct gtcagcaacc ggctcgccgt ttcccactcc tgccccggcc
     9961 aagggctgga gtagttcatc caactgccga taaccgtcag ctctttacgc aatattttgc
    10021 caaacgttgc cgatgttaaa tgcagatcct gatgcaacgt gcccaccagt gccagttggg
    10081 catgaggacc ggcaatctct accgccagtt cgacagtttg cggtacgcca gccgtctcga
    10141 ggataagctg attaaagcgc agttcgcgta aaacgctctg catttgcggc gcgctcattt
    10201 cgctactgtt aaatgtttgc atcgcaccga aagattttgc cagtgccagt ttttctgagc
    10261 taatgtcgat agccgtcaca ctctttgctc ccagcgcgac agcgcactga atagccagca
    10321 ggccaatggt tccggcacca ataataataa cgtttttatt ctcacaacct tgcgctaaat
    10381 gaaaagcatg caggccaacg gtaatcggct caataaaagc cccatcctca ataggcatat
    10441 ccgtgggtag agcaaagaca ttttttcgct taacgacaat atattcggca aatccaccat
    10501 cacgccgtga gccaataaaa tcatattttg cgcactggga ataaaaccct ttcaaacact
    10561 ctggacaagt aaaacagggt aataacggca cacaggcaac cgcatcgcca gggtgtaaat
    10621 catcaacacc ggatcctacc gcatcaatat agccgctaaa ttcatggcct aacgttattg
    10681 gataataatg tgcaccattt ttaaatatcc tgggtaaatc ggaaccacat aagcccgagc
    10741 tggcaatttt tacccgcacc tcatcctgat gtttaatttc aggaatgacg ctttctgcaa
    10801 cgcgcacgat accatcagta tcattcacca ctgatttcat aaaaactcct aattgttaag
    10861 ggggataacc tccccctcag gtaaaaatta ttctgcgaga acgactttct cttgtttaat
    10921 aaagccacgc gctctacgcc aggtcatgaa aataccggta agataaattg cgccgataat
    10981 aatgaaaccg ggaatatttt gcggggagaa aacctgaatc agtaaccagg taatgggaga
    11041 accgccctga tccattgaag ccaccatacc cccggctttt aacgccccag cattagccgc
    11101 cagttgggtg tgcaaaccaa tagtttgcgt cgcgatccac agggtgatgc tcataatgat
    11161 gacacccgag attaaggtgc ggaacagatt tccacgatgc accgcgaccg ccatcgccac
    11221 gaagaagccg atagtggcaa gatcgccaaa cggcagtacc tgattaccag gaacacaaac
    11281 agcaattaaa atggtgagcg gaataaaaat caggcttgcc gataccaccg ccgtatgccc
    11341 cagcagtaat gcgggatcca ggccaatcag gaactcctga ccgccgaact tcgcctgtaa
    11401 acgactacgg gcctgcttag cgatgggcgt taaaccatcc atgatgggtt taatcacccg
    11461 tggcattagc agcatcaccg ccgccgtttt taccgccagc tgcaatacac ctttgacatc
    11521 gtaacccgcg aggatgccga taatcagccc catcacaaaa cccacggtga caggctcgcc
    11581 aaacgggcca aatttgcgct gaatatcgtc ggcgctaaat ttaatacggt taacgcctgg
    11641 gattttctcg atgatagcat cgaccagcac cgcaatcggc cccatatacg ccgacgtacc
    11701 gtgcggaata gcaatgcctt ccagctcaaa gaaatttcgg gtatcgcggg caaaccagtc
    11761 gccgagctta taaacaaacg ccgcgtgaat taccacacct gccatcccta tcatccatga
    11821 accggttgcc agatgcagca acgcgccggt gaaggtcata tgccagatat tccagatatc
    11881 aacatttacc acccgcgtca tacgggtcag taacatcgcc acgttaacca gaatcgcaat
    11941 cggaatcgcc accagcgcaa tttgcgacgc ccaggtcatt ggtgaagagc ccggccagcc
    12001 gacatcgacc acatgcagat tcaggtcgaa attttccgcc atcgctttcg ccgccgggcc
    12061 aatggaatcc agcattaagc caatcacaag gccaatgcca acaaagccaa tcccgatatg
    12121 cagacccgct ttaaagcaat cgcctgcctt catgcctaaa attttagaaa aaataatgat
    12181 gacaatcggc aacatcaccg tagggccgag atcgagaata taacgcatga cttctgaaaa
    12241 cataggtcac ccctgtaaga tagtcagaat tttattttgt aatgcttcga taccgacacc
    12301 agaaacaaaa ggcatgccgt gaactaacgg aatatcgcca aaactacgat ccaccctggc
    12361 agtggtgcat atcaaatgca cgccatccat ataggtttct atttcattaa cccgacactg
    12421 gattaattca acaggaatat tatgactctg acacaactct ttaatttctt ccgccgccat
    12481 cgtagaggtc gcaaccgcgc ctccgcaagc gacaataatc ttgcgtttca taaatagtcc
    12541 tttttattgt tacagaggga tttattcgtc tgaatctaaa acatactttg tgaagtattc
    12601 ctttaactga gtttcaggaa gagtgattag tgtctcaacg atatcgggct gttgtaactt
    12661 gccaaataaa cagcgtaaaa gtttcaattg ctgctgcgga ttttccacaa tcaacgcaat
    12721 aaccaacgat accgccacgt cattatcatc atccgcttgc tgaaaatgaa ctttatttgt
    12781 tggccttaac agataaatgg ctgacgactt agcatgaatc gcctcacaat gcggtattgc
    12841 aatggcgtgc tgctcaagca ttatcccggt agggaattct gcttctctgg caattaatgc
    12901 ctgtggccag gtatcatgaa ccacaccttt ggcgagcatc tcattaccga tatgggttaa
    12961 aacttcgcta cgatcgacaa aagaaattcc gctacgaaca aacaggttag tcatataccg
    13021 tccttattcc gcacagccgt agcgataggc gcgcaaaaca tcataaattt tatccataat
    13081 cagctgatgc ggtattgctg ataattcccc ggactgaatg cgttcaaatt gtttgggaag
    13141 atactgacta atcatgccca gtgggatgtc cacgccttgc agattcacca tcatcgtttc
    13201 aacgctattt ttaatccgac tatgcggcca gtaataacga atacgatccg acaggctgta
    13261 acgaatatcc agtaatgaat cgttaaaacc cgtgcgataa tattttttcc agtattgcgg
    13321 ttcgtcgagc atcacttctt caattaccgc caggcaaccg ctgcgatttt cgggggcgat
    13381 aagttcctgc tcaatttgcg ccagcgcaaa tatcgcctca cgtaaagcaa aggttaatgc
    13441 gggaccgact ttcaatattg caaagtgatc gcggactaat tcccaataag ccgtccgggt
    13501 ctggtaatcg gtagaatgtg cttcataaac cattcgggta ttttctatcc attgcgccag
    13561 cgcctgcgct tcctgcggct gataatggat aatattgctg tgatcaaatt ccacacccgg
    13621 ctgcaccacg atggcaatca cgcgtgttaa tgcctctgtc agcccacggg caataaaggc
    13681 cttttgatgc gtacgtaaag tattggcggc atcttcaaca tgggtgatgt gtactgactg
    13741 aatggcgctg gcctcaccgc ccggaaccgg aacttcggtg ccaatgacat agctcagttg
    13801 ctcacgctgg caatctgtcg ccacactttc cgcagcaaag caaagcacag cagctcgttc
    13861 cgcaaccgtt tctggtgcta acggtatggg atcccccgcg caggacattg acgcatcaag
    13921 atgaatttta ctgaagccag cacgaacata tgcctttacc agctcgacgg atttttccat
    13981 cgccgcatcc acattttctt gctgccagca gtttggcccc agatggtcac cgccgagaat
    14041 aatacgctcg cgtgcaaatc cgactttatc ggcaatcgca aaaacaaatt cgcgaaagtc
    14101 tgccggtgtc attccggtat aaccgccaaa ttgattgacc tggtttgacg ttgcttcaat
    14161 cagcactttg cgcgtgctgt tgcgatcaaa tgccagcgcc gcttcgataa ccaacggatg
    14221 ggcagaacag actgaacata tgccgatatg ttcaccagct ttatgccggg caattaacgt
    14281 tttcatgttt tttccttgtt aaatggcagg tgcgttatgc cctgccctcg cagccacaat
    14341 cggcaatcac tttgctcacc acatcgcgca ttgcggattt agccgactgc aaataatccc
    14401 ggggatcggt cgcttcgggg tgctcggtca ggtaattttt taacgcctgc gagaaggcat
    14461 ttttcagctc cgttgcaacg ttgattttgc atatccccag tttgatggtt tgctgaatat
    14521 ccttagtcga taaccctgac gcgccatgca gcaccagcgg taagttcacc cactggcgaa
    14581 tgttctccag tcgagaaaaa tcaagcgccg gtgcgctggc atacatacca tgagccgtgc
    14641 cgatggcgac cgccagggaa tcaattccgg ttgcctcggc aaattcacgc gcctgagcgg
    14701 ggttggtata aaaagcatcg gcttcattga cttgcacatc atcttcctgg ccgccaagtt
    14761 gccccagctc cgcttcgacg ctgacatcaa agcgatggca aaaatccacc acctctttga
    14821 cccgtgaaat attttgcgca aaaggcaaat gcgaggcgtc aatcatgact gagcgcacgc
    14881 cagaacgaac cttctgagcg atatcgtcaa atttcgtgtg atggtcgaga tgaatcgcca
    14941 gtggatggtg ataatgcttc gccatcgcgc tgaccagcgc caacagattt tctgtaccag
    15001 catgagtaaa tgtgccaggc gttccggcga tgatgaccgg cgcatgcagg ttggcagcgg
    15061 tttctaccac cacttgcatc gtttcgagat tgtgaatatt gaatgccgga accgcgtaac
    15121 cgccgcgctg cgcgttgttc agcatctgct ttgtcgatac cacgtacatt ttcacatcct
    15181 gtcgattgct ttcgatttca aaacataatg aaatcatttg tttttaaatg tcgagataac
    15241 gatcacaaaa acgacaatat gaaaattaat agaggaatga aaggcaaaaa aaacggcctc
    15301 ccggagggaa gccgcaacaa agtgcgcgtg gttttatgca ggatgcggcg taaacatatg
    15361 tggcagcgct gcgtaggcat gataagacgc ttcagcgtcg catcaggcat aggttgccgg
    15421 atgcggcgta aacgccttat ccgtcctacg gggtagtgcc gttcgtatca ggcgatagta
    15481 attttgctat cgagataaac gtcctgcacg gcgttaatca gtttcacgcc gtcagccatc
    15541 gatttcttga acgccttacg tccaagaatc agccccattc cgccagcgcg tttgttgata
    15601 accgcagtac gcactgcatc actgaggtca gtttcaccgc ctgcagcacc accggagttt
    15661 atcaacccgg cccggcccat atagcagtta gctaactgat aacgcaccag atcaatcggg
    15721 ttttcgctgg ttaacttgct gtacacgcga tcgtcggtat aaccgtaatt aattgcttta
    15781 tagccgccgt tattttccgc cattttttgt ttgacgatat ctgcacctat ggttgccgcc
    15841 agatggttcg cctgaccagt caggtcggcg gagacatggt aatcaacgcc atctttctta
    15901 aaggcggagt tacgcaaata ggcccacagc actgtcacca tgcccagctc gtgcgcacgt
    15961 tcaaaagccg cagaaatttc ttcaatctgg cgacgtgact cttccgagcc aaaatagata
    16021 gtcgcaccaa ccgccaccgc gcccatgttg aaggcctgct ccacgctggc atacagcgtt
    16081 tgatcgtagg tattcgggta acttagcgtc tcgttgtgat taagttttac gagaaatgga
    16141 atgcgatgcg catagcgccg cgataccgac gccaacacgc cgtaagttga tgccacacag
    16201 ttacagcccg cttcgatcgc cagttcaaca atgtttttcg ggtcaaagta gagcgggtta
    16261 gcagcaaatg aagctccggc agagtgctca acgccctggt caaccggcag aatagaaaga
    16321 tatcctgtgc cagccagacg cccggtgttg tacaacgtct gcatattacg taacaccgct
    16381 ggcgggcgat tattgtcaat catcacgcgg tctacgtagt catgtccggg gagataaagc
    16441 tggtcagaag gaatagtcat acaacggtgc tgtaaaaggt tgtcggcgtc tttgccaagc
    16501 aactgcgcaa tatctgtcat tactatgctc ccgtaaattc cgattggata tcggctatgg
    16561 attgtcctgg cccgcctttt gcgggcaatc ataatcctgg tcgttacggg cgagtttttc
    16621 catcttttgg atgttttttc agcgttttct gttggctcga ttcatcagaa aaaatgttag
    16681 cgcggtcaag ttttcaccgc aaaggtattt aaaaggtatt attaagtggt attgtcatcg
    16741 cgtaccttac attacctgtc atgaaggaat taaaagatga aaacaacaac aaagctgtcg
    16801 ttcatgatgt ttgttgaatg gtttatctgg ggcgcgtggt ttgtgccatt gtggctgtgg
    16861 ttaagtaaaa gcggttttag tgccggagaa attggctggt cgtatgcctg cacagccatt
    16921 gcggcgatcc tgtcgccaat tctggttggc tccatcactg accgcttttt ctcggcgcag
    16981 aaagtgctgg cggtattgat gttcactggt gcggcgctga tgtatttcgc tgcgcaacag
    17041 accacttttg ccgggttctt cccgttactg ctggcctact cgctaaccta tatgccgacc
    17101 attgcgctga ctaacagcat cgcttttgcc aacgtgccgg atgtggagcg tgatttcccg
    17161 cgcattcgtg tgatgggcac tatcggctgg attgcctctg gtctggcatg tggtttcttg
    17221 ccgcaaatgc tggggtatgc cgatatctca ccgactaaca tcccgctgct gattaccgcc
    17281 ggaagttctg ctctgctcgg tgtgtttgcg tttttcctgc ccgacacgcc gccaaaaagc
    17341 accggcaaaa tggacattaa agtcatgctc ggcctggatg cgctgatcct gctgcgcgat
    17401 aaaaacttcc tcgtcttttt cttctgttca ttcctgtttg cgatgccact ggcgttctat
    17461 tacatctttg ccaacggtta tctgaccgaa gttggcatga aaaacgccac cggctggatg
    17521 acgctcggcc agttctctga aatattcttt atgctggcat tgccgttttt caccaaacgc
    17581 tttggtatca aaaaggtatt attgcttggt ctggtcaccg ctgcgatccg ctatggcttc
    17641 tttatttacg gtagtgcgga tgaatatttc acctacgcgt tactgttcct cggcattttg
    17701 ctgcacggcg taagttacga tttttactac gttaccgctt acatctatgt cgataaaaaa
    17761 gcccccgtgc atatgcgtac cgctgcgcag ggactgatca cgctctgctg ccagggcttc
    17821 ggcagtttgc tcggctatcg tcttggcggt gtgatgatgg aaaagatgtt cgcttatcag
    17881 gaaccggtaa acggactgac tttcaactgg tccgggatgt ggacttttgg cgcggtgatg
    17941 attgccatta tcgccgtgct gttcatgatc tttttccgcg aatccgacaa cgaaattacg
    18001 gctatcaagg tcgatgatcg cgatattgcg ttgacacaag gggaagttaa atgaaaacag
    18061 aacgtattct cggtgctctt tatgggcagg cgttagggga tgcgatgggg atgccctccg
    18121 agctttggcc acgcagccgc gttaaagcac actttggctg gattgaccgt tttctgcctg
    18181 gaccaaagga gaacaacgcg gcctgttatt ttaaccgcgc cgaattcacc gacgatacct
    18241 cgatggcgct gtgtctggcg gatgcgttac tggaacgtga aggcaagatc gacccgaatc
    18301 tgattgggcg taatattctc gactgggcgc tgcgtttcga cgcctttaac aaaaacgtac
    18361 taggtccgac ctcgaagatt gcgcttaacg ccattcgcga cggtaaaccc gttgctgaac
    18421 tggaaaacaa cggcgtgacc aacggcgcag cgatgcgcgt ctcgccatta ggttgtttgc
    18481 ttccggcgcg tgatgttgat tcctttatta atgatgtggc gctggcgtcg agcccgaccc
    18541 ataaatccga tctggcggtt gcgggcgcgg tagtcatcgc atgggcgatt tctcgtgcta
    18601 ttgacggaga aagctggtca gcgattgttg attcattgcc ttcaattgcg cgacatgcac
    18661 agcaaaaacg caccaccacg ttcagcgcct cactggcagc gcggctggag attgcgctga
    18721 aaattgtgcg caatgccgac ggcactgaat ccgccagcga acagctttac caggtcgttg
    18781 gcgcaggtac cagcactatt gagtccgttc cgtgcgccat tgcgctggtt gaactggcac
    18841 aaaccgaccc gaatcgctgc gccgtcctgt gcgctaacct tggcggcgat acagacacca
    18901 tcggtgctat ggcgacggca atctgcggcg cgttgcatgg cattaacgct atcgatcctg
    18961 cgttaaaggc ggagttggat gcggtaaatc agcttgattt caaccgctat gccacagcgc
    19021 tggcgaaata tcgtcaacaa cgggaggcgg catgagcggc gctcgattac acacgctgct
    19081 gcctgaatta accacgcgtc agccggttat ggtgattggc gcggcagtca ttgatgtgat
    19141 cgccgacgct tatgccctcc cctggcgtgg gtgcgatatc gaactgaaac agcagagcgt
    19201 taacgttggc ggctgcgcac tgaatattgc cgtggcgtta aagcgcctcg gcatcgaagc
    19261 gggtaacgcc ttgccgctcg gtcagggcgt gtgggcggag attattcgca accggatggc
    19321 aaaagagggc ttaatcagcc tgatcgataa cgccgaaggt gataacggct ggtgtctggc
    19381 gctggttgag ccggatggcg aacgcacttt tatgtcattc agcggtgtgg aaaatcagtg
    19441 gaatcgccag tggctggcgc gattaaccgt tgcgcctggc agcctgctct atttttccgg
    19501 ttatcaactg gcctcgccct gcggcgaatt attagtggaa tggctggaaa agctgcaaga
    19561 cgtgacgccg tttatcgatt ttggcccacg tattggcgat atcccggagg cattactggc
    19621 gcggatcatg gcctgtcgac ctttagtgtc gctcaatcgt caggaggctg agattgccgc
    19681 cgaacgtttt gctttatccg cagagataac aacacttggc aagcaatggc aggagaaatt
    19741 tgccacgccg ttgatcattc gtctcgataa agagggcgca tggtatttca gcaacgacgc
    19801 ttctggctgc attccggcat ttcccacgca agttgtagac accattgggg cgggcgacag
    19861 tcatgcaggt ggcgtacttg ccgggttggc ctctggtctg ccactggcgg atgccgtatt
    19921 actgggcaat gcagtggcgt cgtgggttgt cgggcatcgg ggcggtgatt gtgcgccaac
    19981 gcgcgaggaa ctactcctcg cacacaaaaa cgtatagatc gctgcgacag tggctaatgc
    20041 tgtactcaat aggccgttgc tgttggtcaa gcgccacttg cttgatcacc agcaccggta
    20101 ttttgctatc taactgaata tgtgactgaa actcggcatc tggcatccgg gcgctaacgc
    20161 gggaacgtgt acgttgtggg taaatgtgct ggctgcggaa atagtcataa agagaaattc
    20221 cgatggcatc aacatcatga attaaatgcg ccggaaccca cgattcctca atcgataccg
    20281 cttcttcatc gacatagcga atacgcttaa gcaaaaagac atcacttccc gcttcgaccg
    20341 ccagctgctg cgcgacttct tcgggacatt tcacgacacg cttgttaacc catagcgtat
    20401 cgggcttttt cccgcgcaat accacctgct gagaaaaacc tcgcgcttct ttcaacgagt
    20461 attcgaagat gttgttgatc tgcgtgccgt aaccgcgcga acgcgtcacc acaccctctt
    20521 cttccagcgc ctgcatcgcc ttgcgcaccg taatgcgtga aacgccggtt aactgactta
    20581 aatcacgctc accgggcaaa atattgccat gctccagcac cccgctgcgc acggcatttt
    20641 ttaccgtttc ggcaaactta atataaagcg gcgtgttatc cgccgctaaa atacgctcgt
    20701 tcagttgggc aatcagctgg gtatgcgctt gttccatttg tctttttcct gccagggagt
    20761 ctggtgggag tatatcactg cttcttattt aaggcgtttc tttaatccca tcaacgaatg
    20821 cctgcaactc ctcctctgtg ccattaaaca cattaaaatc caccgaacca ttaatgccat
    20881 ctacctgccc acggtcggaa tgctgccaga aacgccaggc catgccgtca ttgtccgggc
    20941 gacgttgata gtagtgagcc acccaccacg gatattcatt gaaatagccc gccagattgg
    21001 tgtgataaaa aacggctcct gagtaaataa ccggcttttt tcccgtactt ttttcgacca
    21061 tttttagcca ctgacttacc cgcttgcgta attcttttgc cgataatttt ccccgttctt
    21121 ctacgtccag cacggcaggg aaatcgcctt gtgagaaatc caccgtttgc agaaataatc
    21181 gcgcctgaac aggcgctgct accgacgggg agaaataatg atacgccccg cgcagcaggc
    21241 cattttcgcg gcttagttgc cagttacgcg aaaaataggg gtccaccagc ttttcgcctt
    21301 ccgtcgcctt gataaaggca aattgtaagc ggataccgtt atcgcgcatt tttgccaccc
    21361 gctgccagtc gatccgctcc tgccagcgcg aaacatctat tccgtgaatg gtgtaactgg
    21421 caggaatatg aatagcgaaa gatttaaccg ggcgataacc gtagaagtgg atgtagtctg
    21481 taatctgaac tgctgtcgaa tagaaaaaat tcaccgtctg acgcggataa attgcaacga
    21541 tggaaattag cccaagagcg cataatagag cggtgagttt ttttcgactg gtaattctta
    21601 gctgcattgg tttcatccct gaatgtcagt gccaaaggct gacaataacc aaagcagcta
    21661 tagtacggtg ctcaccacca ggcgtggaag tggtgaaccg gaccaatacc gtgaccaact
    21721 tccagcgtgt cggcctgggc taacgccgat gaaagccagc tttttgcctc ctgtacggtg
    21781 tcagcccagt ttgtatggcg cgggcgtagt gcagccaacg ccgcagagag tgtacaacca
    21841 gtgccgtggg tgtttttggt cataatgcgc ggtgcggtaa accgttgttc accctcgcgg
    21901 gtaaacagcc agtccgggct ttgctcatca tccagatggc cacctttcat tagcactgcg
    21961 ccacagccca tcgccaacag cgctcgccct tgttccagca tttcctgttc ggtgcgcgcg
    22021 tgtggcgcgt cgagcaaggc agcagcttcg ggcaagtttg gcgttattaa tgaaacctgt
    22081 ggcaataatc gactgcgtag cgtggcaact gccgaaggtg aaagcagtgg gtcgccactt
    22141 tttgccagca taacggtgtc gagtaccacg ttttggatct gataacgttg caaccgttct
    22201 gccaccgctt caacaatatc ggtttccgcc aacataccga ttttagtggt atcgattcgc
    22261 acatcgctga acaccgaatc gagctgggcg gcgacaaaat caggctcaat gcgatacacc
    22321 gactgtacgc cacgggtatt ttgcgccacc agtgcagtaa taactgagca accataagcg
    22381 ccaagtgccg agaaggtttt aagatcggcc tgaatccccg caccaccact cggatcagta
    22441 ccggcaatcg tcagagcgtt aattcgtttc atgcctgcac ctcctgcgtc aattgccaga
    22501 gcgcatcaag gaaatgtgga acaaaactgc ctggcccctc gcttctggcg actgcgcgtt
    22561 ctccggcttg tttcatccag tgacaggcag atgcgacatt ttccagcata tcgcctggta
    22621 acgcacaaca ggcagcgaca accgccgata atgcacagcc agttcctacc actttggtca
    22681 ttaacggatc gccaccgtga atgccaacgg cgcgatggcc atcggtaaca taatccacct
    22741 cgccagtgac cacgacgatt gcgccagttt cccgtgccag tgtttgtgca gcgggtatcg
    22801 cgttaactgc ggcgtcagtg gtatccactc cccgtccacc attagcaacg ccagctaatg
    22861 ccatgatttc cgaagcatta ccacgtatcg ctgccggttt aaaagataaa agttcatgac
    22921 aaaaatggcg gcgataatcg agcgcaccaa ccgctactgg atcaagcgtc cagggtgttt
    22981 gagagctttt tgcttgctca acggcagcac gcatcgcctg tgcgcgtggc tgcgtcagtg
    23041 tgccaacgtt aatcaacaag gcactggcga tagccgcaaa ctgactggcc tcttcggttt
    23101 cgataaccat cgctggcgat gcaccgagcg ccagcaaggt attggcagta aaggtttgca
    23161 ccacatcgtt ggtcatgcag tgcacaagag gggaatgttg gtgaaaaagg tgtaacgtgt
    23221 gcgcagattg cgcggaactc agcaggtcga cttgcatagt ttgctcctgc cataacgtga
    23281 agaagcaatg acctggtggt ccgtgacttc cctacgctgg cattatccag atcaggtgat
    23341 acgggtattt ctcagccttc acgcagaagg gcaccccgag tcgtttggtt gaatctctat
    23401 attttgtgcc agcttagggg aagaggcttg cagatgcaag ctcgcctcca ttcattacgt
    23461 cttcactgtg taccccatat cagtaatgaa aaagaaacaa ttcacaccca tcattcaggt
    23521 ggtaaaaact
//
LOCUS       DABSYT020000068        23249 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.068, whole genome shotgun sequence.
ACCESSION   DABSYT020000068 DABSYT020000000
VERSION     DABSYT020000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.068"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            201..2186
                     /gene="yjcS"
                     /locus_tag="ID725_RS15985"
                     /old_locus_tag="pgaptmp_003197"
     CDS             201..2186
                     /gene="yjcS"
                     /locus_tag="ID725_RS15985"
                     /old_locus_tag="pgaptmp_003197"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGV7851735.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            2395..2670
                     /gene="ytcA"
                     /locus_tag="ID725_RS15990"
                     /old_locus_tag="pgaptmp_003198"
     CDS             2395..2670
                     /gene="ytcA"
                     /locus_tag="ID725_RS15990"
                     /old_locus_tag="pgaptmp_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGV7851736.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
     gene            2689..3720
                     /gene="mdtN"
                     /locus_tag="ID725_RS15995"
                     /old_locus_tag="pgaptmp_003199"
     CDS             2689..3720
                     /gene="mdtN"
                     /locus_tag="ID725_RS15995"
                     /old_locus_tag="pgaptmp_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGV7851737.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            3720..5771
                     /gene="mdtO"
                     /locus_tag="ID725_RS16000"
                     /old_locus_tag="pgaptmp_003200"
     CDS             3720..5771
                     /gene="mdtO"
                     /locus_tag="ID725_RS16000"
                     /old_locus_tag="pgaptmp_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGV7851738.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            5768..7234
                     /gene="mdtP"
                     /locus_tag="ID725_RS16005"
                     /old_locus_tag="pgaptmp_003201"
     CDS             5768..7234
                     /gene="mdtP"
                     /locus_tag="ID725_RS16005"
                     /old_locus_tag="pgaptmp_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGV7851739.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            7432..9579
                     /gene="fdhF"
                     /locus_tag="ID725_RS16010"
                     /old_locus_tag="pgaptmp_003202"
     CDS             7432..9579
                     /gene="fdhF"
                     /locus_tag="ID725_RS16010"
                     /old_locus_tag="pgaptmp_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7849..7851,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HGV7851740.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            9673..10362
                     /gene="yjcO"
                     /locus_tag="ID725_RS16015"
                     /old_locus_tag="pgaptmp_003203"
     CDS             9673..10362
                     /gene="yjcO"
                     /locus_tag="ID725_RS16015"
                     /old_locus_tag="pgaptmp_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGV7851741.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(10540..11853)
                     /gene="gltP"
                     /locus_tag="ID725_RS16020"
                     /old_locus_tag="pgaptmp_003204"
     CDS             complement(10540..11853)
                     /gene="gltP"
                     /locus_tag="ID725_RS16020"
                     /old_locus_tag="pgaptmp_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGV7851742.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12195..12791)
                     /gene="nrfG"
                     /locus_tag="ID725_RS16025"
                     /old_locus_tag="pgaptmp_003205"
     CDS             complement(12195..12791)
                     /gene="nrfG"
                     /locus_tag="ID725_RS16025"
                     /old_locus_tag="pgaptmp_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGV7851743.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(12788..13171)
                     /gene="nrfF"
                     /locus_tag="ID725_RS16030"
                     /old_locus_tag="pgaptmp_003206"
     CDS             complement(12788..13171)
                     /gene="nrfF"
                     /locus_tag="ID725_RS16030"
                     /old_locus_tag="pgaptmp_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGV7851744.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(13164..14882)
                     /gene="nrfE"
                     /locus_tag="ID725_RS16035"
                     /old_locus_tag="pgaptmp_003207"
     CDS             complement(13164..14882)
                     /gene="nrfE"
                     /locus_tag="ID725_RS16035"
                     /old_locus_tag="pgaptmp_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGV7851745.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            complement(14902..15858)
                     /gene="nrfD"
                     /locus_tag="ID725_RS16040"
                     /old_locus_tag="pgaptmp_003208"
     CDS             complement(14902..15858)
                     /gene="nrfD"
                     /locus_tag="ID725_RS16040"
                     /old_locus_tag="pgaptmp_003208"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGV7851746.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(15855..16526)
                     /gene="nrfC"
                     /locus_tag="ID725_RS16045"
                     /old_locus_tag="pgaptmp_003209"
     CDS             complement(15855..16526)
                     /gene="nrfC"
                     /locus_tag="ID725_RS16045"
                     /old_locus_tag="pgaptmp_003209"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGV7851747.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(16523..17089)
                     /gene="nrfB"
                     /locus_tag="ID725_RS16050"
                     /old_locus_tag="pgaptmp_003210"
     CDS             complement(16523..17089)
                     /gene="nrfB"
                     /locus_tag="ID725_RS16050"
                     /old_locus_tag="pgaptmp_003210"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGV7851748.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(17134..18570)
                     /gene="nrfA"
                     /locus_tag="ID725_RS16055"
                     /old_locus_tag="pgaptmp_003211"
     CDS             complement(17134..18570)
                     /gene="nrfA"
                     /locus_tag="ID725_RS16055"
                     /old_locus_tag="pgaptmp_003211"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGV7851749.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSHDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            18963..20921
                     /gene="acs"
                     /locus_tag="ID725_RS16060"
                     /old_locus_tag="pgaptmp_003212"
     CDS             18963..20921
                     /gene="acs"
                     /locus_tag="ID725_RS16060"
                     /old_locus_tag="pgaptmp_003212"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGV7851750.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            21122..21436
                     /gene="yjcH"
                     /locus_tag="ID725_RS16065"
                     /old_locus_tag="pgaptmp_003213"
     CDS             21122..21436
                     /gene="yjcH"
                     /locus_tag="ID725_RS16065"
                     /old_locus_tag="pgaptmp_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGV7851751.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            21433..23082
                     /gene="actP"
                     /locus_tag="ID725_RS16070"
                     /old_locus_tag="pgaptmp_003214"
     CDS             21433..23082
                     /gene="actP"
                     /locus_tag="ID725_RS16070"
                     /old_locus_tag="pgaptmp_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGV7851752.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         5598 a         6301 c         6089 g         5261 t
ORIGIN      
        1 agtcaggtca cattattgat gatagtaata aatatgtcgt acttttccct cttcagcaca
       61 aatgcctaat aaaatggatc gttccgctca cacttccact tattaaagtc gcaatttccc
      121 aattatgata acgccagctt taaatgacgt attttgcatg gattacatat ttacgacata
      181 aatgataaat ggagatttat atgaataact ctcggttatt ccgtttgagc aggattgtta
      241 ttgcgttaac tgccgccagc ggcatgatgg taaataccgc taacgcgaaa gaggaagcga
      301 aagccgccac tcaatatacc caacaggtta atcagaatta cgccaaatca ttaccgttta
      361 gcgatcgtca ggattttgac gatgcccagc gtggatttat cgccccgctg ctggatgaag
      421 gtattctgcg tgatgcgaac ggtaaagttt actaccgcgc agacgattac aaatttgata
      481 ttaatgccgc tgcgccggaa accgtaaacc ccagcctgtg gcgtcagtcg cagatcaacg
      541 gcatttctgg cctgttcaaa gtcaccgata aaatgtatca ggtgcgcggc caggatatct
      601 ctaacattac gttcgttgag ggggagaaag gcattattgt catcgacccg ctggtgacgc
      661 cgcctgccgc aaaagccgca cttgaccttt acttccagca tcgtccgcaa aaaccgattg
      721 ttgccgttat ctacactcac agccacaccg accactatgg tggcgtgaaa ggcattatct
      781 ctgaagccga tgttaaatcc ggcaaagttc aggtgattgc ccctgctggc tttatggacg
      841 aagccatcag cgaaaacgtg ctggcgggta acatcatgag ccgccgtgcg ctctactctt
      901 acggtctgtt actgccgcac aacgcgcaag gcaacgtggg taatggcctt ggcgtgacgc
      961 tggcaacggg cgacccgagc attattgcac cgactaaaac tatcgtcaga actggcgaga
     1021 agatgattat cgacggcctg gagtttgact tcctgatgac cccaggtagc gaagcgccag
     1081 ccgaaatgca cttctatatt ccggccctga aagccctgtg taccgccgag aacgccacgc
     1141 ataccctgca caacttctac actctgcgcg gcgcgaaaac ccgcgatacc agcaagtgga
     1201 ccgagtacct gaacgaaacg ctggatatgt ggggtaacga cgcggaagta ctgtttatgc
     1261 cgcacacatg gccggtctgg ggcaataagc atatcaatga ttatattggt aaatatcgcg
     1321 atactatcac gttcattcac gaccagaccc tgcacctggc gaaccagggc tacaccatga
     1381 atgaaatcgg cgacatgatt aagctgccgc ctgcacttgc caataactgg gccagccgtg
     1441 gctattacgg ttctgtcagc cacaacgccc gcgcggtgta taacttctat cttggctatt
     1501 acgacggtaa cccggctaac ctgcatccgt atggtcaggt agagatgggc aaacgttacg
     1561 tgaaagcgct gggcggctct gcccgtgtca tcaacctggc gcaagaagcg aataagcaag
     1621 gtgattaccg ctggtcggca gaactactga aacaggtgat tgccgccaac ccgggtgacc
     1681 aggtggcgaa gaatctgcaa gcgaataact ttgaacagct gggctatcag gccgagtccg
     1741 ccacatggcg cggtttctac ctgaccggcg cgaaagagct gcgcgaaggg gtgcataagt
     1801 tcagccacgg caccaccggt tccccggaca ccattcgcgg aatgtcggtc gaaatgctgt
     1861 tcgactttat ggccgttcgc ctcgatagcg cgaaagctgc gggtaaaaat atcagcctga
     1921 acttcaatat gagcaacggc gataacctca acctgacgct gaacgatagc gtgcttaact
     1981 accgtaaaac actgcaaccg caagccgacg cctctttcta catcagccgt gaagatctgc
     2041 acgccgtgct gaccggacag gcaaaaatgg cggatctggt gaaagcgaag aaagccaaaa
     2101 ttattggcaa tggcgcgaaa ctggaagaaa ttatcgcctg tctggataat ttcgatttgt
     2161 gggtgaatat cgtaacccca aattaatgtc actcgctttt gggggcgctg aagcccccaa
     2221 aagcatttat tttaaaaata tatacagcca attaataaat aaaatatata agttatgaat
     2281 gcggtggctg aataaagcta tttttattca tcaagaaaat agccatactg acgcattcaa
     2341 tggatattga tattcactac agatattgtt cgcagcagac aggatgtatt taatatgcca
     2401 acagttctct ctcgcatggc gatgcaactc aaaaaaacag cctggataat tcccgtcttc
     2461 atggtttcgg gatgctcatt atctccggca atcccggtga tcggcgctta ttatcccagc
     2521 tggtttttct gcgccattgc cagccttata ttgacgctca tcacgaggcg agttattcag
     2581 cgggcaaata tcaagctggc atttgtcgga attatttata ccgccctttt tgctctctac
     2641 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
     2701 ccgaaaaaag ctcctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
     2761 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgtg
     2821 tcagcagata ccattgatgt ggtgccggaa gtcagcggcc gcattgtaga actggcggtc
     2881 accgacaacc aggcagtcaa acagggcgat ttgctgttcc gcattgaccc gcgcccgtac
     2941 gaagccaatc tggcgaaatc tgaagcctcc ctcgcggcac tggataagca aattatgctc
     3001 acccagcgta gcgttgacgc gcaacagttt ggtgccgact cggttaacgc cacggtagaa
     3061 aaagcccgtg ccgccgctaa acaggccaca gatacattac gccgcaccga gccattactg
     3121 aaagaaggtt ttgtctcagc ggaagacgtt gaccgtgcga ggaccgcgca gcgcgctgca
     3181 gaagccgatc ttaatgctgt attgttgcag gcgcagtcag ccgccagcgc cgtcagcggc
     3241 gtggatgcat tagttgccca gcgtgcggcg gtcgaagcgg atattgccct gaccaaactg
     3301 catctggaaa tggcgaccgt tcgcgcaccg tttgatggcc gggtcatttc cctcaaaacc
     3361 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctaatcga cactcgtcac
     3421 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacacct
     3481 gcaacgattc gcctgatgag tgacagcggc aaaactttcg agggtaaagt ggattcaatt
     3541 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc gaaagtttct
     3601 cgttctatta actgggtccg tgttgcccag cgttttccgg tcaaaatcat ggttgataaa
     3661 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     3721 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     3781 agttaagcga gcggcgacca ggtcgcgtgc cgcagaccgt gcaactctgg gtaggctgcc
     3841 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     3901 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtcgcg atcttgtttg
     3961 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     4021 aaccgttgat ccgcttgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     4081 gcacccatcg gttgggactg gtctttttcg ctgtcgccat tgtcgccatt tatgggcaaa
     4141 ccttccccgc catgctcgac tatccggaag tggtcgtgcg cttaacgctg tggtgtatcg
     4201 ttgttggcct ctacccgacg ttattaatga cgttaatcgg cgtactgtgg tttcccagtc
     4261 gtgccatttc gcaaatgcat caagcgctta atgatcggct tgatgatgcc attagccacc
     4321 tgacggacag cctcgcaccg ctacccgaaa cgcggattga aagagaggcg ctggcgctgc
     4381 aaaaactcaa tgtcttttgc ctcgcggacg atgccaactg gcgaactcaa agcgcatggt
     4441 ggcaaagctg cgtggcaacg gtaacctaca tttactcgac gctgaatcgc tacgatccca
     4501 cctcttttgc tgattctcag gcaattattg aattccgaca aaaattagct tcagaaatca
     4561 acaagctgca gcatgccatt accgaaggtc agtgctggca aagcgactgg cggatcagtg
     4621 aaagtgaagc gatggcggca cgggaatgta acctggagaa tatctgccag acgttgttac
     4681 aactgggtca gatggacccg aatacgccgc caacgcccgc cgccaaaccg ccatcaatgg
     4741 tcgctgatgc ttttaccaat ccagactata tgcgctacgc ggtaaaaacg ctgctcgcct
     4801 gtttgatctg ttacaccttc tacagcggcg tggactggga aggcattcac acctgtatgc
     4861 tgacctgcgt gatcgtcgct aatccgaatg tcggttcatc gtaccagaag atggtgctgc
     4921 gttttggcgg ggccttttgc ggcgcgattc tggcgctgtt attcacgcta ctggtcatgc
     4981 cctggctgga caatattgtc gaattgctgt ttgtgctggc accgattttc ctgttgggcg
     5041 catggattgc caccagctct gaacgctctt cttatatcgg cacacagatg gtggtcacct
     5101 tcgcgctcgc cacgctcgaa aacgtttttg gtccggtgta cgacctggtg gaaattcgcg
     5161 atcgcgccct gggtatcatc attggtaccg tggtgtctgc ggtgatttac acctttgtct
     5221 ggcctgaaag tgaagcgcgc acgctgccgc aaaaactggc tggcgcgctg ggtatgctaa
     5281 gtaaagtaat gcggatccca cgccagcagg aagtcacggc tctgcgcact tatctgcaaa
     5341 ttcgtatagg tctgcatgcg gcgtttaatg cctgtgaaga gatgtgccaa cgcgtggcgc
     5401 tggagcgtca actggacagc gaagaacgcg ccttactgat tgaacgttcg caaacggtta
     5461 ttcatcaggg ccgcgatctt cttcacgcct gggatgccac ctggaactcg gcgcaggcgc
     5521 tggataacgc actacagccg gacaaagcag gtcagtttgc cgacgccctg gaaaaatacg
     5581 ctgccggtct ggcaaccgca ctcagccgtt ctcctcaaat aacgcttgaa gagacacccg
     5641 cctcgcaggc catcctgccc accttattaa aacaggagca acacgtctgc cagcttttcg
     5701 cccgcttgcc agactggaca gccccggcat taacgcccgc cacggaacag gcacaaggag
     5761 ccacgcaatg atcaatcgtc aactttcacg tctgctgttg tgcagcattc tcggcagcac
     5821 gacgctgatt tccggctgtg ccctggtacg caaggattct gcgcctcatc aacagctcaa
     5881 accggaacaa atcaaactgg ccgatgatat tcatcttgcc agctccggct ggccgcaggc
     5941 gcagtggtgg aaacaactca atgacccgca gctggatgcg ctgatccaac ggacgctaag
     6001 tggttcacac accctcgccg aagcgaaact gcgggaagaa aaagcgcagt cccaggccga
     6061 tttgttagat gccggttcac aattacaggt cgcagcgtta gggatgctca accgccaacg
     6121 cgtctcggcg aacggctttt taagccctta tgcgatggat gcgccagcac tgggtatgga
     6181 cgggccgtac tatacggaag ccacagtcgg tttgtttgcc ggactggatc ttgatttgtg
     6241 gggtgtgcat cgctcagcgg ttaccgccgc cattggcgcg cataatgccg cgctggcaga
     6301 aaccgcagca gtagagctat cgctgaccac gggcgtagcg cagctttatt acagtatgca
     6361 ggccagctat cagatgctcg atctgttaga acaaactcgc gatgtgattg attacgcggt
     6421 gaaagcgcac caaagtaaag tggcgcacgg tctggaagcg caagtgcctt tccacggcgc
     6481 gcgggcacag attctggcgg tcgataaaca aattgctgcc gttaaagggc aaatcaccga
     6541 aacgcgagaa tctctgcgtg cattgattgg cgcgggagcc agcgatatgc cggagatcaa
     6601 accagtggca ttaccacgag tccagaccgg tattccggcg acactctctt atgagttgct
     6661 cgccagacgc ccggatctgc aagccatgcg ctggtatgtt caggcgtcat tagatcaggt
     6721 ggattccgcg cgggcgctgt tctacccaag ctttgatatc aaagcgtttt tcggtctgga
     6781 ctccatccac ctggatacct tattcaaaaa aaccagtcgc cagttcaact tcatcccggg
     6841 tctgaaattg ccgctgtttg acggtggacg gttgaatgcc aatctcgaag gcactcgcgc
     6901 cgccagcaac atgatgattg aacgttacaa ccagtcagta ctgaacgcgg tgcgtgacgt
     6961 tgccgtcaac ggcacgcgtc tgcaaacgct caacgacgag cgagaaatgc aggctgaacg
     7021 cgtggaagca acgcgcttca cccagcgcgc tgccgaggcc gcctatcagc gcggcttaac
     7081 cagccgctta caggctaccg aagcccggtt gccggtgctt gccgaagaga tgtcattact
     7141 gatgctggac agccgccggg tgatccaaag cattcagttg atgaaatcgc tgggcagcgg
     7201 gtatcaggcg gctcccgtcg tcgagaaaaa ataaaatgtc tgccgtgtga tggctgtcac
     7261 gcggtatttc gtttcgtcac gtcaaaactg acgacagcct gtttttcgtc agagttttga
     7321 ataaatagtg cccgtaatat cagggaatga ccccaaataa aatgtggcat aaaagatgca
     7381 tactgtagtc gagagcgcgt atgcgtgatt tgattaactg gagcgagacc gatgaaaaaa
     7441 gtcgtcacgg tttgccccta ttgcgcatca ggttgcaaaa tcaacctggt cgtcgataac
     7501 ggcaaaatcg tccgggcgga ggcagcgcag gggaaaacca accagggtac cctgtgtctg
     7561 aagggttatt atggctggga cttcattaac gatacccaga tcctgacccc gcgcctgaaa
     7621 acccccatga tccgtcgcca gcgtggcggc aaactcgaac ctgtttcctg ggatgaggca
     7681 ctgaattacg ttgccgagcg cctgagcgcc atcaaagaga agtacggtcc ggatgccatc
     7741 cagacgaccg gctcctcgcg tggtacgggt aacgaaacca actatgtaat gcaaaaattt
     7801 gcgcgcgccg ttattggtac caataacgtt gactgctgcg ctcgtgtctg acacggccca
     7861 tcggttgcag gtctgcacca atcggtcggt aatggcgcaa tgagcaatgc tattaacgaa
     7921 attgataata ccgatttagt gttcgttttc gggtacaacc cggcggattc ccacccaatc
     7981 gtggcgaatc acgtaattaa cgctaaacgt aacggggcga aaattatcgt ctgcgatccg
     8041 cgcaaaattg aaaccgcgcg cattgctgac atgcacattg cactgaaaaa cggctcgaac
     8101 atcgcgctgt tgaatgcgat gggccatgtc attattgaag aaaatctgta cgacaaagca
     8161 ttcgtcgctt cccgtacaga aggctttgaa gagtatcgta aaatcgttga aggctacacg
     8221 ccggagtcgg ttgaagatat caccggcgtc agcgccagtg agattcgtca ggcggcacgg
     8281 atgtatgccc aggcgaaaag cgccgccatc ctgtggggca tgggtgtaac ccagttctac
     8341 cagggcgtgg aaaccgtgcg ttctctgacc agcctcgcga tgctgaccgg taacctcggt
     8401 aagccacatg cgggtgttaa cccggttcgt ggtcagaaca acgtgcaggg tgcctgcgat
     8461 atgggcgcgc tgccggatac gtatccggga taccagtacg taaaagatcc ggctaaccgc
     8521 gagaaattcg ccaaagcctg gggcgtggaa agcctgcctg ctcataccgg ttatcgcatc
     8581 agcgagctgc cgcaccgcgc agcgcatggc gaagtgcgtg ccgcgtacat tatgggcgaa
     8641 gatccgctac aaactgacgc ggagctgtcg gcagtacgta aagcctttga agatctggaa
     8701 ctggttatcg ttcaggacat ctttatgacc aaaaccgcgt cggcggcgga tgttatttta
     8761 ccgtcaacgt cgtggggcga gcatgaaggc gtgtttactg cggctgaccg tggcttccag
     8821 cgtttcttca aagcagttga accgaaatgg gatctgaaaa cggactggca aatcatcagt
     8881 gaaatcgcca cccgtatggg ttatccgatg cactacaaca acacccagga gatctgggat
     8941 gagttgcgtc atctgtgccc ggatttctac ggtgcgactt acgagaaaat gggcgaactg
     9001 ggcttcattc agtggccttg ccgcgatact tcagatgccg atcaggggac ttcttatctg
     9061 tttaaagaga agtttgacac cccgaacggt ctggcgcagt tcttcacctg cgactgggta
     9121 gcgccaatcg acaaactcac cgacgagtac ccgatggtac tgtcaacggt gcgtgaagtt
     9181 ggtcactact cttgccgttc gatgaccggt aactgtgcgg cactggcggc gctggctgat
     9241 gaacctggct acgcacaaat caataccgaa gacgccaaac gtctgagtat tgaagatgag
     9301 gcattggttt gggtgcactc gcgtaaaggc aaaattatca cccgtgcgca ggtcagcgat
     9361 cgtccgaaca aaggggcgat ttacatgacc taccagtggt ggattggtgc ctgtaacgag
     9421 ctggttaccg aaaacttaag cccgattacg aaaacgccgg aatacaaata ctgcgccgtg
     9481 cgcgtcgagc cgatcgccga tcagcgcgcc gccgagcagt acgtgattga cgagtacaac
     9541 aagttgaaaa ctcgcctgcg cgaagcggca ctggcgtaat acagtccttt ctacagcctc
     9601 ctttcggagg ctgttttttt atccattcga actctttata ctggttactc cctacccaat
     9661 cgtattatca aaatgaaaaa aattatcgca ttgatgttgt ttttgacatt ctttgcccac
     9721 gccaacgact ccgagcctgg cagccagtat ttaaaggcag cagaggccgg ggaccgacgc
     9781 gcacaatatt ttcttgccga cagctggttt agctccggcg atttgagcaa agccgaatat
     9841 tgggcacaga aagccgccga cagcggtgat gctgatgcct gcgcgctgtt ggcgcagatc
     9901 aaaatcacca atccggtcag tctggactat ccacaagcaa aagttcttgc agagaaagcg
     9961 gcgcaagcgg gcagtaaaga aggtgaagta acgctggcgc atattctggt aaatactcaa
    10021 gcgggtaaac cggattatcc aaaggcgatt tcgctgttag aaaacgcctc ggaagatctg
    10081 gagaacgact ctgccgtcga tgcccaaatg ctgcttggtt tgatttacgc caacggcgtg
    10141 ggcattaagg ccgacgatga caaggcaacc tggtatttca aacgcagctc tgcaatttcc
    10201 cgaaccggtt attccgagta ctgggcggga atgatgttct taaacggtga agaaggtttt
    10261 atcgagaaga acaagcaaaa ggcgctgcac tggttgaacc tgagctgtat ggaagggttt
    10321 gataccgggt gtgaagagtt tgaaaaatta actaacggtt aaggtaggcc tgataagacg
    10381 cgttaagcgt cgcatcaggc gttgatgtcg gatgcagcgt aaacgcctta tcagacctac
    10441 aaaatcacgc tagcgctgtc tgacgtaggc ctgataagac gcaccagcgt cgcatcaggc
    10501 atccacacat tgccggggga tatccccggc aatcttcaat tattgatccg cagttttatc
    10561 aaatttgccc agcacttcac gctcataagc cagcgccttt ttgcggtcaa atttgtgttc
    10621 ccacttggca atcaccagca ccgccagcgc attacccacc acgttcagtg cagtacgcgc
    10681 catgtcgagg atacggtcaa caccagcaat aaacgccaga ccttccagcg gaatacctac
    10741 gctacccagc gttgccagca acaccacaaa cgacacgccc ggcacgccag caatcccttt
    10801 cgaggtcacc atcagcgtca gcaccagaat gatttcctgc cagatggaca ggtcaatgcc
    10861 atacagctga gcgatgaaaa tagcggcaat actttgatac agcgtcgaac catcaaggtt
    10921 aaaagagtaa ccggtcggca ccacgaagct ggtgatcgac gccggtgctc cgtaggcttc
    10981 catcttctca ataattcgcg gcagtacgct ttcagagctg gcagtggagt acgccagaat
    11041 cagctcatct ttcagaatac gaatcaggat ccagacgctt aacccgcata ggcgcgccac
    11101 aattcccagc actaccagcg cgaagaacag aatggcgaaa tgcaccagca gcaccagttt
    11161 cgccagtggc cacagagaag agaaaccaaa gttagccacc gtcactgcaa tcagcgcaaa
    11221 cacacccacc ggcgcataac gcatcaccat gtgagtcact ttaaacatgg tttcagagat
    11281 ggagcggaac acggtcacca gcggttcacg atgcgtcgcg ggcagggagg aaagccccag
    11341 accaaacagc accgagaaga agatgatcgg caacatttcg cctttcgcca tcgacgccac
    11401 aatattcgtc ggcaccagcg acaaaatcgt gcccataatg ccgtgggaac tgctttgtac
    11461 cgcttccgta gtgctctgat atttcgagat atcgacggtc gccagctgcg acatatccac
    11521 cccggcaccg ggctggaaga cgttcgccag agtgatcccc aaaatgatgg caacggtggt
    11581 gatcacctcg aagtagataa tggttttcgc gccaatacgc ccgagctgtt tggcatcacc
    11641 aacacccgcg atacccacca ccagcgtgga gatcacaatc ggcacaacaa tcattttgat
    11701 cagatggatg aagatatcac ccgccggaga gagcaaattg acgaccagcc agtcgcggct
    11761 gtcgctatgg tagtgcaggt agcttcccag gagaatgccc agcaccatag caaacagaat
    11821 ctgccaggcc aggctgaatt ttatattttt catatgaatg acttcctcaa tggagcgccg
    11881 ttccccgttc agaaacgcga gaacgtgaca tactgccagg gcatgaattg tgttgcgttg
    11941 gtttgtagat ttttttaacg cttatgatca gtatcatttg cgcggtatgt tgcgcaaccc
    12001 tttaacaaca gagcttttcg ctgcattaat gcataataac gcgagtttat ggcaggatta
    12061 atgaataacc atttgttatg gaaagatttt tatttgttta atctgcaata atactgatta
    12121 gtgattattt ttggcatatg ttcattcgat tattttttat gcagctcaat aacataatct
    12181 tcatcaccag atgattaatc cgatctccgc tgcaacaatt tcgccatatt aatcgactcg
    12241 accagctgtg tccggttgat ccggggtgag ttgagatcca tcactttttg ccatagttcg
    12301 atggcttgcg cgtaattcgc ctgcataaac gcatcggaag ccagcagcat cagagcggtg
    12361 atttcgttag agtccagcgc gagggctttg tcgatcattg cgcgagtctg ggcggtcata
    12421 tgctggctgg cctggtaata aagcaccgtc gccagcgccg catacagttc agcgttctca
    12481 ccacgtagtt gcaacgcctg acggtacgcc agcagcgaat tgctgtaatc gttttgccac
    12541 agatagtact cgcccagtaa cgcccactgt tcgctgtttt gtggattagc gcggatttta
    12601 tcctgcaatg cctgaagctg cgcttccggg ttttgctggc tggcaaactg atgtagcgga
    12661 tcgcgctgac gctgatactc cgcacgtacc gcctgccatt ttgggcttaa cagataacta
    12721 ccgatacaga ggaataccat caatatcgtg agtgttgtca gcattttcac cggtatttga
    12781 ggctgtttca tcgcttcgcc ctcactcgcc agaggatcag tgccatcagc agtaacaaca
    12841 ccactggcag cgcccacaac accagcgtct gacccgttaa cggtgggtta tagcgaacaa
    12901 aatctccgta gcgttcggtc atccagccaa tgatttcgac ttcacttttc ccctccgcca
    12961 ccatgctgta aacctgatgg cgcatactga ctgccaccgg cgcgttggat tccagtaagt
    13021 tttgattctg gcactgcgga caacgtaact ggctggcaat atttaacgcc tgttgctgtt
    13081 gttgcggatt ggcgaattgc caggtgtcta caacctgagc gcgcgcaaaa caggtaaata
    13141 acagcagtag cgtgagaagc cctttataca tcgcgcttct tcccccgcca tccgcttaac
    13201 aatgcgcccg caatcatcaa caatcctccc ccccagatcc agcgcacacc gctttgtaca
    13261 tacaagcgaa aagcgtaacg atccgctcca gttttctccc ccatgaccgc ataccagtca
    13321 tggatgccgt tccagcgaat tgacggttcc atcatttgct gacgacgagc ttcgtaaaaa
    13381 cgccgctccg gcgttaattc accaatgcgt tgctgatggt caaacagcgc cactatcgct
    13441 ttttcgctgg tgtaattgcc tttggcttgc agatcgaggc gctcaaaacg gaaggtgtat
    13501 cctgccagcg tcacctgctg acccggctgt aaattcaggc tgatctcctg acggctgacg
    13561 ctggagacaa cgatccctgc ggcaaataac agtatgcccg catgcgccag cagcgccgga
    13621 agctgcgcgc gtttgccaga gacaaacgtc gccagcacaa tcaccaccaa catcaacaga
    13681 ccaaacggta acgtcgcgcg gttaaaatac ggtgcgccaa cggagagacg tccccagccc
    13741 agcaggccgt aaatcatcgg ataaagcgtt cccaccagca cgatcagtag cactgcgcaa
    13801 aacagcaaca gcgtagcgag gattaacatt tcccgcgata accccgaaaa acgcaccgcc
    13861 gggccaccgt cccgcgctcg ccagccatac agagccagag acgcaaggct aatcagtgca
    13921 aacaggctga acaacggcac agcgcgaacg ttatccagag cgaacgcatg aaccgaaacc
    13981 agaatgccag aacggacaat taaggtgccc agcagcgaca gcatcagagt aactatcgcc
    14041 aacaacagcg accagtggcg aaaaatcccc cgctggcgtg tcagggataa actgtgcagc
    14101 agcgcagtgg cagaaagcca gggtaataaa gaggcgtttt ccaccggatc ccagaaccac
    14161 cagccgcccc agccgagttc gcagtaggcc caccaggaac cgaggatgat ccccgcagtt
    14221 aatgcactcc agccaggtaa cgcccagcgc cagcaaattc gggcgcaggc accatcaaac
    14281 tcgccgcgca gtagactcgc cagcgccacg ctcgccgcta ccatcaaacc gccatagcca
    14341 aggtaaagca gcggtggatg aaagataaga ccgggatgtt gcagcatcgg attgagatcg
    14401 cggccttcga ttgctggtgg aaatatccgc acgaagggat cggaccacag cactacaaac
    14461 agtagcagtg cggcgagcat gagagataaa acggctagcg tcagcggaaa tagcggatcg
    14521 gtttgctgcc gatattgcca ggcaaacagc gcgctccagg cggaaagcag cagcacccaa
    14581 agcagcagcg aaccttcatg accgccccac accgccgcca gtttgagttt ccacgacagc
    14641 aagctgtagc tatgttgggc gacataaatg accgagaaat cgctgatgag aaaacaatac
    14701 gtcagtacgc caaaggcgag cagcaggagc gcgaactgtg ccagaatgcc gatgcaagtg
    14761 agtcgcatca tggcaggcca gcgcaaccgc actcccgcaa aggccgtcaa cggggtcaac
    14821 acgttgaccc cgagacttaa caacagcgcc agaaaaccga cttcaggaag gaatacgtcc
    14881 agaaaccacc tctgttctgg cttacgccac cgttaactgc ccggcgtaga gaatgaaaaa
    14941 tcgcaacatc aacacgccga ccagactggc cccacacgcc gccagcacgg caggaatgcc
    15001 ggaactgcga ttgacccacg gtttgaacaa cattggcaca atcagcccca gcccggcgac
    15061 accaagccag aaccaccacg tccagaaacc gccgcccaat gccgccacca gcgcacgcac
    15121 tttaccgtca tcgcccagcg ccagaccgac aaaaaacgcc gccagcagga agatttcacc
    15181 ccataccacg gggatttcca tacggtgtac aaactgcgct tccgtggaat gcgggttact
    15241 gcgttggcgt atcgccatgg cgatcagtgc caccgccgca ccggacgaga tgccggagaa
    15301 gaggaacagc accggcagga tcgggttatt aaggaacgga tacgatttca gcgccgacag
    15361 cagaaaaccg gtataagccc ccaacaacac cgccagcacc agcatcaatg tttccagtcc
    15421 gcgatgaacg ggcgtcagca ggctcagaac cttttgcacg atccccagct tcggcaacca
    15481 gcgttgttgc agggcaagca aatcatgttc aaagattttc gccagccaca gcaccagcac
    15541 caccatgtaa agctgaaaca gcatcacccc catcgacatc accgaagtaa aactgtagtg
    15601 gaacatcagc ttccagaagg tccacggtct tgtcaggtgg aagacgaggc tcaacagacc
    15661 gaggatcacc gcgccaggtc cgacaatcag cgtggtgcgc agcaacgtgc tgtctgcacc
    15721 gcccgcctgc gggtagaagc gacgtaacag cacggccagc gtcaccagac cggcagaaat
    15781 accaatcaaa aacaggtaga tggcaatcgg ccagtcccac accagcgatt caaaatgaaa
    15841 tgcggaagtc tgcgtcattg gctcacctcc ccgtatttaa acggtacgcg gtacagcttc
    15901 ggtttggttc ccagcgccag cttgtagcgg taagtgggct tctggcgcag cagttgcgaa
    15961 atctcactgt tgggatcgtc cagattgcca aacgtcagcg ccttcgtcgg gcaagcctca
    16021 acgcacgcgg gaagcttacc ggcctgcaaa ttggtcttac ggcagaaatc gcatttgtcc
    16081 gctgtcttcg tgaccggatg aataaagcgc acgcggtacg gacaggcggc gatgcagtac
    16141 tgacaaccga cgcagagatc cggattaaca tcgacaatcc cactggcagc atcgcgaaaa
    16201 gatgcaccgg tcgggcagac gtcaacgcac ggcgcatgat cgcagtgctg gcaagactta
    16261 cggaagaagc gatatttcac atcaggaaat tcgccctgcg gctcgctacg aataatcgtc
    16321 aggcgcgaga cgccttccgg caccttgttc acttcccgac aagcatccat acaggcggta
    16381 cagccgatgc ataacgactc gtcatgcacc atgccgtaac gcaccccatt gatattcaac
    16441 gttttcgcca ccacgcgccc tgcggtgccg ctgacggctg ccagcacgcc gacgccggtg
    16501 agaaactggc gacgagacca ggtcatggct gctccttaag caacggaaca gacgccgggt
    16561 taaagttcgg attggtgcgc tgatcgctgt ggcaatcgac gcaaatctta atccgtcctt
    16621 tgtcacttaa cgtctgcatc gtatcttgct gtggatgcag ggaatggcag ctggcgcacg
    16681 ccactttggt tacgtggaca tcgtgcggcc agaacgcttt ttgcaactgt tccggcagat
    16741 ggcaggacat acagacgctg ttctgctctc caaccttgta catcggctcg ttaaagcgca
    16801 tcacatcttt caccccttcg cggtgttgcg gtgatggctg gccgtgacag ttggtgcagg
    16861 tgaccggcag tttgttattc gggttgataa cggaagcatg tttgccatgc ataccttcgg
    16921 tatctggctt atgacagtcc agacaggcgg catccgggtt acgctgctgg gtaacttcgt
    16981 agcgatcatc agatgcctgc gccgcagggg tagcggtaat tccgttcagg ctccacaaca
    17041 ggcccgacgc cagcaccccg gcagttaata acgaacgtaa tacgctcata ttcactccat
    17101 tgtgttttcc gccccttgcg aggcggaacg gggttattgg cttaacagac cgtttttacg
    17161 tgcctgctct tcccactgcg ggatcaccgt tttgatgaag tcctgcttct cggccttgat
    17221 ttgttccatg ttcaggccaa tggcctgctg ggctttctct ttggtcgaga tatccgggat
    17281 ctggatttca tgggtgatgc ctttagtcgc cagcaggcgc gccagtttgg tgcgtgcatc
    17341 cgccgcttta tccatcgccg taccgagcat cctcagacct tcttccggtg cgtgcatatg
    17401 aatgccgtgg gaagcgatcg ccagatccca acgccactgg gcatgacgga tatcgtcctg
    17461 aatcggcttc atttcggctt ccgtcgcacc tgcgtccagc gccgctttcg cttcgaagtg
    17521 agcgtgaacc agttgatctt caaccttgat tttcaggtcg ttaatcgact gcttacgttc
    17581 cgcgaccact ttttgcaagg cagctttgtc ctgggtatgg cagttcgcac aagtctgggc
    17641 gaagttatca aacggattac caattttatg gtcggtgtag agtttgcctt cggcgttctg
    17701 cacttttggc atatggcagt cgatacaggt cacgttgttt ttaccgtgaa tgcccgctgt
    17761 ccaggtttca tattccgggt gctgcgcttt cagcattggc gttttcgaca gggagttagt
    17821 ccagtcagag aaggcaattt tgtcgtaata ctgctccata ttttcgactt tcatgccgtc
    17881 atcccacggg aatttaaccg ctttgttttt gccgtcgaag taatactcca catggcactg
    17941 accgcaaacc atcgattgct ggtcgaaacg tccggctttc tcaaaaggtt taccaatggc
    18001 ttccatcgcg cgagccgcat acggacggga aagggttaac tccggtttgc ctttggcgaa
    18061 ctctggagag gcggtgttat ggcaatcggc acaacctaag ttgttgacga tttccgggcc
    18121 gccgcgcgcc catttaccgt ggaagtagcc atcttcgccg tctttctgga tcagacgcgc
    18181 cacatccggg cttttacaac tccagcacgc catcggtagc ggaccatctt cagcgttttt
    18241 cggcgcaccg gtacgcaggg tttcacgcac atcggtcaca gcaaaggcat gtccacgcgg
    18301 cttgttgtaa tcgtgcgaga agggataccc cgcccacagg atcaccagcc gtggatcttc
    18361 cgccagggcg tcaacacgct ctgactgttc cgaggtggct ttccaggaga gatattgatc
    18421 gggatgctgc ggggcaaagg tttcattctt cgcttccaca gttacaggtt ttgcgggagc
    18481 agccgtttgt tcagcgtgaa cagaagtgaa aaagaaaaaa ggaatcaata agctgaagat
    18541 acggcgtgcg tttattttta tccttgtcat aggggcttca tccgaattgc gccattgttg
    18601 caatggcggt ttttattgtt tttcacgaca gtaaccgcac ctacactgtc atgacattgc
    18661 tcgcccctaa gtgtaacaaa taaccacact gtgaatgttg tctttaatca attgtaagtg
    18721 catgtaaaat accactttag agttagtcag tatcttcctc tttttcaaca gcatgcataa
    18781 ctgcatgttc ctcaaagaat taatcaactt ttgttgctga ccttcaaaaa ttaccctgcc
    18841 gtttatttgc acaattctac ttttgcgtga tctgtcgccc aaatactaaa caaaactgcc
    18901 aataccccta catttaacgc ttatgccaca tattattaac atcctacaag gagaacaaaa
    18961 gcatgagtca aattcacaaa cacaccattc ctgccaacat cgcagaccgt tgcctgataa
    19021 accctcagca gtacgaggcg atgtatcaac aatctattaa cgcacctgat accttctggg
    19081 gcgaacaggg aaaaattctc gactggatca aaccgtacca gaaggtgaaa aacacctcct
    19141 ttgcccccgg taatgtgtcc attaaatggt acgaggacgg cacgctgaat ctggcggcaa
    19201 actgccttga ccgccatctg caagaaaacg gcgatcgtac cgccatcatc tgggaaggcg
    19261 acgacgccag ccagagcaaa catatcagct ataaagagct gcaccgcgac gtctgccgct
    19321 tcgccaatac cctgctcaag ctgggcatta aaaaaggtga tgtggtggcg atttatatgc
    19381 cgatggtgcc ggaagccgcg gttgcgatgc tggcctgcgc ccgtattggc gcggtgcatt
    19441 cggtaatttt cggtggcttc tcgccggaag cggttgccgg gcgcattatc gattccaact
    19501 cacgactggt gatcacttcc gacgaaggcg tgcgcgccgg gcgtagtatt ccgctgaaga
    19561 aaaacgttga tgacgcacta aaaaacccga acgtcaccag cgtagagcat gtggtggtac
    19621 tgaagcgtac tggcgggaaa attgactggc aggaagggcg cgacctgtgg tggcacgacc
    19681 aggttgagca agccagcgat cagcaccagg cggaagagat gaacgccgaa gatccgctgt
    19741 ttattctcta tacctccggt tctaccggaa aaccaaaagg cgtactgcac actaccggcg
    19801 gttatctggt gtacgcggcg ctgaccttta aatatgtctt tgattatcat ccgggcgata
    19861 tctactggtg caccgccgat gtgggctggg tgaccggaca cagttatttg ctgtacggcc
    19921 cgctggcctg cggcgcgacc acgctgatgt ttgaaggcgt accgaactgg ccgacgcctg
    19981 cccgtatggc acaggtggtg gacaagcatc aggtcaatat tctctatacc gcgcccacgg
    20041 cgattcgcgc gctgatggcg gaaggcgata aagcgatcga aggcaccgac cgttcgtcgc
    20101 tgcgcattct cggttccgtg ggcgagccaa ttaacccgga agcgtgggag tggtactgga
    20161 aaaaaatcgg caacgagaaa tgtccggtgg tcgatacctg gtggcagacc gaaaccggcg
    20221 gtttcatgat caccccgctg cctggcgcta ccgagctgaa agccggttcg gcaacacgtc
    20281 cgttcttcgg cgtgcaaccg gcgctggtcg ataacgaagg taacccgctg gaaggggcta
    20341 ccgaaggtag cctggtgatc accgactcct ggccgggtca ggcgcgtacg ctgtttggcg
    20401 atcacgaacg ttttgagcag acctattttt ccaccttcaa aaatatgtat ttcagcggcg
    20461 acggcgcgcg tcgtgatgaa gatagctatt actggatcac cgggcgtgtg gacgatgtgc
    20521 tgaacgtctc cggtcaccgt ctgggaacgg cggagattga gtcggcgctg gtggcgcatc
    20581 cgaaaatcgc cgaagccgct gtcgtcggta ttccgcacaa tattaaaggt caggcgatct
    20641 acgcctacgt cacgcttaat cacggggagg aaccgtcacc agaactgtac gcagaagtcc
    20701 gcaactgggt gcgtaaagag attggcccgc tggcgacgcc agacgtgctg cactggaccg
    20761 actccctgcc taaaacccgc tccggcaaaa ttatgcgccg tattctgcgc aaaattgcgg
    20821 cgggcgatac cagcaacctg ggcgatacct cgacgcttgc cgatcctggc gtagtcgaga
    20881 agctgcttga agagaagcag gctatcgcga tgccatcgta acccacaatt gccggatgcg
    20941 agtcggtaac ggtttgtagg cctgataaga cgcgacagcg tcgcatcagg cattgattgc
    21001 cggatgcggc gtataacgcc ttatccggcc tacattcggc aagggttacc cgagcgttaa
    21061 ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg gagaatctgt
    21121 gatgaatggc actatttatc agcggataga agacaatgcg catttcaggg agttagtcga
    21181 aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt atatcggctt
    21241 tattttactg atcgccttcg cgcccggctg gctgggcacc ccgctgaatc cgaacaccag
    21301 cgtcacacgc ggtattccag tcggtgttgg ggtaatagtg atctcctttg ttctcactgg
    21361 tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag tcctgcatga
    21421 ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc tttcgcagct
    21481 aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg gcaggcgatt
    21541 attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc gtcaaaacgc
    21601 gtacgttcgc gtaatgacta ttacaccgca ggcggcaata tcaccggttt tcagaacggg
    21661 ctggcgattg ccggtgacta tatgtccgcc gcctcatttt tggggatctc cgcactggtg
    21721 tttacctccg gctatgacgg cttaatttac tcgctgggct tcctggtggg ctggccgatc
    21781 attttgttcc tgattgccga acgtctgcgt aacctggggc gttacacctt tgccgatgta
    21841 gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg ttctctggtg
    21901 gtggtggcgc tttaccttat cgcccagatg gtgggggcag gtaaactgat cgagctgctg
    21961 tttggcctta actatcacat tgcagtggtg ctggtcggcg tgctgatgat gatgtacgtc
    22021 ctgttcggcg gcatgctggc gaccacctgg gtacagatta tcaaagctgt gctgttgctg
    22081 ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag cttcaacaat
    22141 ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa accgggcgga
    22201 ctggtgaaag atccgatctc cgcactctct ctggggctag gactgatgtt tggtacggct
    22261 ggcttgccgc acattctgat gcgcttcttt acggtcagcg atgcccgcga agcgcgtaaa
    22321 agcgtgttct acgctaccgg atttatgggc tacttctata tcctgacctt tattatcggc
    22381 ttcggcgcga tcatgctggt cggtgcgaat ccggaatata aagacgcggc gggccatctg
    22441 attggtggta acaacatggc agcagttcac ctggccaatg cggtgggcgg caacctgttc
    22501 ctcggcttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc gggtctgacg
    22561 ctggcgggcg catcagcggt ttcgcatgac ctgtacgcta acgtcttcaa aaaaggcgcg
    22621 accgaacgtg aagagctgcg ggtatcgaaa atcaccgtcc tgattttggg tgtgattgcg
    22681 attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtggg gctggcgttt
    22741 gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ctatgtactg gtcgaaactg
    22801 accacgcgtg gcgcgatgat gggtggctgg ctggggctga ttaccgcagt ggtactgatg
    22861 atcctcggcc cgacgatttg ggtacagatc ctcggtcacg aaaaagccat cttcccgtat
    22921 gaatacccgg cgctgttctc tatcaccgtg gcattcctcg gcatctggtt cttctcggca
    22981 accgataact cagcggaagg cgcgcgtgag cgtgaactgt tccgcgcgca gtttatccgc
    23041 tcccagaccg gctttggcgt tgagcaaggc cgcgcgcatt aatctctccc cttccccggt
    23101 cgtctgaccg gggaatactc ttcctctccc aaatgcatca ccttttccca taatattaaa
    23161 caaataaact cattaaaaaa aaatgagcga tttttgacag tcgtagaaaa tgataatgca
    23221 gagaatatgc cttttctttc ttgttaatt
//
LOCUS       DABSYT020000069        23047 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.069, whole genome shotgun sequence.
ACCESSION   DABSYT020000069 DABSYT020000000
VERSION     DABSYT020000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.069"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            52..414
                     /gene="yibL"
                     /locus_tag="ID725_RS16075"
                     /old_locus_tag="pgaptmp_003215"
     CDS             52..414
                     /gene="yibL"
                     /locus_tag="ID725_RS16075"
                     /old_locus_tag="pgaptmp_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGV7851753.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            972..1640
                     /locus_tag="ID725_RS16080"
                     /old_locus_tag="pgaptmp_003216"
     CDS             972..1640
                     /locus_tag="ID725_RS16080"
                     /old_locus_tag="pgaptmp_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGV7851754.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVYAKEFARWQQ
                     LLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
     gene            1684..6450
                     /gene="ehaG"
                     /locus_tag="ID725_RS16085"
                     /old_locus_tag="pgaptmp_003217"
     CDS             1684..6450
                     /gene="ehaG"
                     /locus_tag="ID725_RS16085"
                     /old_locus_tag="pgaptmp_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HGV7851755.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            6818..8473
                     /gene="lldP"
                     /locus_tag="ID725_RS16090"
                     /old_locus_tag="pgaptmp_003218"
     CDS             6818..8473
                     /gene="lldP"
                     /locus_tag="ID725_RS16090"
                     /old_locus_tag="pgaptmp_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGV7851756.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            8473..9249
                     /gene="lldR"
                     /locus_tag="ID725_RS16095"
                     /old_locus_tag="pgaptmp_003219"
     CDS             8473..9249
                     /gene="lldR"
                     /locus_tag="ID725_RS16095"
                     /old_locus_tag="pgaptmp_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGV7851757.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            9246..10436
                     /gene="lldD"
                     /locus_tag="ID725_RS16100"
                     /old_locus_tag="pgaptmp_003220"
     CDS             9246..10436
                     /gene="lldD"
                     /locus_tag="ID725_RS16100"
                     /old_locus_tag="pgaptmp_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGV7851758.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10622..11095
                     /gene="trmL"
                     /locus_tag="ID725_RS16105"
                     /old_locus_tag="pgaptmp_003221"
     CDS             10622..11095
                     /gene="trmL"
                     /locus_tag="ID725_RS16105"
                     /old_locus_tag="pgaptmp_003221"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGV7851759.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(11148..11969)
                     /gene="cysE"
                     /locus_tag="ID725_RS16110"
                     /old_locus_tag="pgaptmp_003222"
     CDS             complement(11148..11969)
                     /gene="cysE"
                     /locus_tag="ID725_RS16110"
                     /old_locus_tag="pgaptmp_003222"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGV7851760.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(12049..13068)
                     /gene="gpsA"
                     /locus_tag="ID725_RS16115"
                     /old_locus_tag="pgaptmp_003223"
     CDS             complement(12049..13068)
                     /gene="gpsA"
                     /locus_tag="ID725_RS16115"
                     /old_locus_tag="pgaptmp_003223"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGV7851761.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(13068..13535)
                     /gene="secB"
                     /locus_tag="ID725_RS16120"
                     /old_locus_tag="pgaptmp_003224"
     CDS             complement(13068..13535)
                     /gene="secB"
                     /locus_tag="ID725_RS16120"
                     /old_locus_tag="pgaptmp_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGV7851762.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(13598..13849)
                     /gene="grxC"
                     /locus_tag="ID725_RS16125"
                     /old_locus_tag="pgaptmp_003225"
     CDS             complement(13598..13849)
                     /gene="grxC"
                     /locus_tag="ID725_RS16125"
                     /old_locus_tag="pgaptmp_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGV7851763.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13991..14422)
                     /gene="yibN"
                     /locus_tag="ID725_RS16130"
                     /old_locus_tag="pgaptmp_003226"
     CDS             complement(13991..14422)
                     /gene="yibN"
                     /locus_tag="ID725_RS16130"
                     /old_locus_tag="pgaptmp_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGV7851764.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14667..16211
                     /gene="gpmM"
                     /locus_tag="ID725_RS16135"
                     /old_locus_tag="pgaptmp_003227"
                     /gene_synonym="pgmI"
     CDS             14667..16211
                     /gene="gpmM"
                     /locus_tag="ID725_RS16135"
                     /old_locus_tag="pgaptmp_003227"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGV7851765.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            16245..17504
                     /gene="envC"
                     /locus_tag="ID725_RS16140"
                     /old_locus_tag="pgaptmp_003228"
     CDS             16245..17504
                     /gene="envC"
                     /locus_tag="ID725_RS16140"
                     /old_locus_tag="pgaptmp_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGV7851766.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            17508..18467
                     /gene="yibQ"
                     /locus_tag="ID725_RS16145"
                     /old_locus_tag="pgaptmp_003229"
     CDS             17508..18467
                     /gene="yibQ"
                     /locus_tag="ID725_RS16145"
                     /old_locus_tag="pgaptmp_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGV7851767.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(18454..19489)
                     /gene="waaH"
                     /locus_tag="ID725_RS16150"
                     /old_locus_tag="pgaptmp_003230"
                     /pseudo
     CDS             complement(18454..19489)
                     /gene="waaH"
                     /locus_tag="ID725_RS16150"
                     /old_locus_tag="pgaptmp_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(19728..20753)
                     /gene="tdh"
                     /locus_tag="ID725_RS16155"
                     /old_locus_tag="pgaptmp_003231"
     CDS             complement(19728..20753)
                     /gene="tdh"
                     /locus_tag="ID725_RS16155"
                     /old_locus_tag="pgaptmp_003231"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGV7851768.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            complement(20763..21959)
                     /gene="kbl"
                     /locus_tag="ID725_RS16160"
                     /old_locus_tag="pgaptmp_003232"
     CDS             complement(20763..21959)
                     /gene="kbl"
                     /locus_tag="ID725_RS16160"
                     /old_locus_tag="pgaptmp_003232"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGV7851769.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(22234..>23047)
                     /gene="yibB"
                     /locus_tag="ID725_RS16165"
                     /old_locus_tag="pgaptmp_003233"
     CDS             complement(22234..>23047)
                     /gene="yibB"
                     /locus_tag="ID725_RS16165"
                     /old_locus_tag="pgaptmp_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HGV7851770.1"
                     /translation="RGDWTANKGFREKLARSVDVYFSYFERLAALENEMIIFTSPDLK
                     PRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKNPEYWSPEY
                     VLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMHLFT
                     IKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVDD
                     DQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
BASE COUNT         5927 a         5912 c         5773 g         5435 t
ORIGIN      
        1 ctggtcgcgc atactagggg ctataaattt atctttatca gaagccatca catgaaagaa
       61 gtcgaaaaaa acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag
      121 gccgatctgt cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc
      181 acgctggaag cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa
      241 gcacagaagc tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat
      301 atgggcaagc tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg
      361 ggccgtgaaa tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct
      421 cagacgactg gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg
      481 gactctcctg tctactcgcc taattaccaa acaattcact ccctttacgt cgatttaccg
      541 ctggttgata ttctgaaaat gcatttttac cgaaatagcc acctataaac aataaaaata
      601 tatagaagat atcatttatt aattaacaag cagttacgtc tctttttttc ggatttaatt
      661 gtgaaatttt aatcatcata ttcatacaat aaattatctg taaatttttt cccagacatg
      721 gtttctttca tatttgacat ttattaataa tcatcattag aatccacgaa aataatatat
      781 tcatacaaac acagacatct tttgaaacga atgatacatt tttataatga atgcttaaaa
      841 caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagat caccaataat
      901 acaatggaca actctaccgg cattgatcgc tgggcggagg aaataaggat ttttacatga
      961 actagaagaa gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat
     1021 gtgactacat tgaagaaggc aagccagaaa gtagtttatt gaaacaacaa gaagaacata
     1081 acaataaaat tgatctactt gaaaaacaac aagcacagct aaagagtcaa ctggaaacta
     1141 ttcaaaaaca acaaactggc ataataagca gtacaaagac tttaactcac gtaattaaat
     1201 ccgttaaaga tcaacaaaac acttttattt ttaccgagtt taacccagca aaaacgaagt
     1261 atttcatcct gaacaatggt tcggtggctt tagcgggtcg agttttatcc attgacgcca
     1321 cagagaatgg cagtgttatt catatttcac tggtcaactt attaagtaca cctatctcaa
     1381 atattggttt taatgcgaca tggggtggtg aaaaacctgt ctatgccaaa gagtttgccc
     1441 ggtggcaaca attacttttc aacacatcaa tgaaatccac attgaaatta ttaccaggtc
     1501 aatggcaaga cattaatttg accctgaagg gtgtatcgcc caataacctg ggatatctga
     1561 agttagccat caacatggaa aatattcagt tcgacaatct tccctctgct gaaaatcggc
     1621 agaaaagaag caaaaaataa atgcagttag tacgcttata acaaggaata gcgagcatca
     1681 ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta tactgttacc
     1741 agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg
     1801 ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac
     1861 ggtcagggtg ttgattacgg tagtggatca gctggcgacg gctgggttgc tataggcaaa
     1921 ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt gggttatgac
     1981 gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc gattggtggt
     2041 gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat agcactgggt
     2101 gcctcttcgt attcattggg ccaatactca atggccctcg gccgttattc aaaagcattg
     2161 ggtaaattgt ctattgctat gggggactct tccaaagcgg aaggagcaaa cgccattgcc
     2221 ctgggaaatg ccactaaagc tactgagatt atgagtattg ctcttggcga caccgccaat
     2281 gcgtcaaaag cgtattcaat ggcgctggga gcaagtagcg tcgcatctga agaaaacgct
     2341 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
     2401 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
     2461 actatcgcat taggaaatgg cagccaggct ctggcagata atgcaatcgc cataggccag
     2521 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
     2581 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
     2641 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
     2701 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
     2761 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
     2821 attagcgact cggtagcaaa aaggcttgga ggaggggctg cagtagatgt tgatgacggt
     2881 actgttacag caccaaccta caatttaaaa aatggtagca aaaataacgt aggggctgcg
     2941 ctcgctgtac ttgatgaaaa caccctgcaa tgggaccaaa ccaaaggcaa atacagcgct
     3001 gctcatggta ctagtagccc aactgccagc gtaatcaccg atgttgcgga tggcacgatt
     3061 tcagcctcca gtaaggatgc ggttaacggt tcccaactga aagctaccaa tgacgatgtc
     3121 gaagccaaca ccgccaatat cgctactaat accagcaaca ttgccacgaa tacggcaaat
     3181 attgccacca ataccaccaa tatcaccaac ctgacggatt ccgttggtga ccttcaggct
     3241 gatgccctgc tctggaacga aactaaaaag gcattcagtg cagctcacgg ccaggatacc
     3301 accagcaaaa tcaccaacgt taaagatgcc gacctgacgg ctgacagcac tgatgctgtt
     3361 aacggctctc agctgaaaac caccaacgat gctgtggcga cgaataccac caatatcgcc
     3421 aataacactt ccaatattgc cactaacacc accaacatct ctaacctgac tgagacggtg
     3481 actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtatt cacggcagct
     3541 catggcaccg agaccaccag caaaatcacc aacgttaaag atggcgacct gacgactggc
     3601 agcaccgatg ccgttaacgg ctctcagctg aaaaccacca acgatgccgt ggcgacgaat
     3661 accaccaata tcgccactaa caccaccaac atctctaatc tgactgagac ggtgactaat
     3721 cttggtgagg atgcgctgaa atgggataag gacaatggtg tcttcactgc agctcatggc
     3781 aacaataccg ccagcaaaat caccaatatc ctggacggca cagtcactgc aaccagttcc
     3841 gatgccatta acggtagcca gctttatgac ttaagcagca atatcgccac ctacttcggc
     3901 ggcaatgctt ctgtgaatac tgacggtgtg tttaccggtc caacctacaa aatcggtgaa
     3961 acaaattatt ataacgtcgg cgatgcactg gctgcgatta actcctcatt tagcacgtct
     4021 ctcggcgatg ctctgctttg ggatgccacc gcaggtaaat tcagtgccaa acacggtact
     4081 aatggtgacg caagcgtgat cactgatgtc gcagatggtg aaatttcaga ctccagttct
     4141 gacgcagtaa acggctcaca actccacggc gtgagcagtt atgttgttga tgcgctgggg
     4201 ggtggtgccg aagtcaatgc agacggcacc atcactgcgc cgacgtacac cattgctaat
     4261 gctgattacg ataatgtcgg tgatgccctg aatgctatcg ataccactct tgacgacgct
     4321 ctgctctggg atgcggacgc cggtgaaaat ggtgcattta gcgccgctca cggaaaagat
     4381 aaaactgcca gtgtaatcac taacgtcgct aacggtgcaa tctctgctgc cagcagcgac
     4441 gcgattaacg gctcacaact ctataccacc aataagtaca tcgctgatgc gctgggtggt
     4501 gacgcagaag tcaacgctga cggcaccatc accgcaccga cttacaccat tgcgaacgcc
     4561 gagtacaaca acgtcggtga cgccctggat gcgcttgatg ataacgccct gctgtgggat
     4621 gagactgcca atggcggtgc tggagcctac aatgccagcc atgacggtaa agccagcatc
     4681 atcactaatg tcgctaatgg cagtattagt gaggacagta ccgatgcagt gaacggttct
     4741 cagttgaatg cgacgaatat gatgattgag cagaacaccc aaattatcaa tcagctcgct
     4801 ggtaacaccg acgcaaccta tatccaagaa aacggtgcgg gtattaacta tgtgcgtact
     4861 aacgacgacg gcttagcgtt caacgacgcc agcgcacagg gtgttggcgc tacagctata
     4921 ggttataact ctgtcgccaa aggcgatagc agcgtagcta ttggtcaggg cagctacagc
     4981 gacgttgata cgggtatcgc cctgggtagc agctctgttt ccagccgagt gattgccaaa
     5041 ggctcccgtg acaccagcat aacggaaaat ggcgttgtta ttggttacga caccacggat
     5101 ggcgaactgc tcggtgcatt gtctatcggt gatgacggta aatatcgtca aatcatcaac
     5161 gtagccgatg gttccgaagc ccatgacgcc gttacggttc gtcaattgca gaatgcgatt
     5221 ggtgcggtcg caaccacgcc gactaaatac ttccacgcta attcaacgga agaagattca
     5281 ctggcagtgg gaactgactc gctggcaatg ggtgcgaaaa ccatcgtgaa tggcgataaa
     5341 ggtattggta tcggttatgg tgcctacgtg gacgcgaatg cacttaacgg cattgccatt
     5401 ggtagcaatg cgcaagtcat tcatgtcaac agtattgcga taggtaatgg ttctacgacc
     5461 actcgtggcg ctcaaaccaa ttataccgcc tacaacatgg acgcaccgca gaactctgtc
     5521 ggtgaattct cagtcggtag tgcggatggt caacgtcaga tcactaacgt cgcagcaggt
     5581 tcggctgata ccgatgcggt caacgtgggt cagttgaaag taacggatgc gcaggtttcc
     5641 cagaataccc agagcattac taacctggat aatcgggtaa cgaatcttga ttcacgcgtc
     5701 accaatatcg aaaacggtat tggcgatatc gtcaccaccg gtagcaccaa gtacttcaag
     5761 accaataccg atggtgtaga tgccagcgcg cagggtaaag atagcgtcgc gattggttcc
     5821 ggctccattg ctgccgctga caacagcgtc gctctgggta cagggtctgt ggcaaccgaa
     5881 gaaaatacga tctctgtagg ttcctctact aaccaacgtc gtatcaccaa cgtagctgca
     5941 ggtaaaaatg ctaccgatgc tgttaacgtg gcacagttga agtcttccga agctggcggt
     6001 gtacgttacg acaccaaagc tgatggttct atcgactata gcaatatcac cctcggtggc
     6061 ggcaacggcg gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa caacgacgtg
     6121 gtgaattacg cgcagttgaa gcaaagcgtg caggaaacga agcaatacac cgatcagcga
     6181 atggttgaga tggataacaa actgtctaaa actgaaagca agttgagcgg tggtatcgct
     6241 tctgcaatgg caatgaccgg tctgccgcag gcttacactc caggtgccag catggcctct
     6301 attggtggcg gtacttacaa cggtgaatcg gcagttgctt taggtgtatc gatggtgagc
     6361 gccaatggtc gttgggtcta caaattacaa ggtagtacca atagccaggg tgaatactcc
     6421 gccgcactcg gtgccggtat tcagtggtaa tgatccatta agttagtgtg actaagggca
     6481 aacgccaaat tgcctgatgc gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca
     6541 atctaagggc aattctctga tgaggattgc ccttttcttt accagacatc tccccccaca
     6601 agaattggcc ctaccaattc ttcgcttatc tgacctctgg ttcacaattc cccaattaaa
     6661 actcacatca atattgccaa tacataacat ttagttaacc attcattgtc attatcccta
     6721 cacaacacaa ttggcagtgc cacttttaca caacgtgtga caaggagatg agcaacagac
     6781 tcattacacg atgtgcgtgg actccaggag acctgcaatg aatctctggc aacaaaacta
     6841 cgatcccgcc gggaatatct ggctttccag tttgatagca tcgcttccca tcctgttttt
     6901 cttctttgcg ctgattaagc tcaaactgaa aggatacgtc gccgcctcat ggacggtagc
     6961 aatcgccctt gccgtggctt tgctgttcta taaaatgccg gtcgctaacg cgctggcctc
     7021 ggtggtttat ggcttcttct atggtttgtg gcccatcgcg tggatcatta ttgcagcagt
     7081 gttcgtctat aagatctcgg tgaaaaccgg gcagtttgac atcattcgct cgtctattct
     7141 ttcgataacc cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt
     7201 ccttgaagga gccgcaggct ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg
     7261 cctgggtttt aaaccgctgt acgccgccgg actgtgcctg attgttaaca ccgcgccagt
     7321 ggcatttggc gcgatgggca ttccaatcct ggttgccgga caagtaacag gcatcgacag
     7381 ctttgagatt ggccagatgg tggggcggca gctaccgttt atgaccatta tcgtgctgtt
     7441 ctggatcatg gcgattatgg acggctggcg cggtatcaag gagacgtggc ctgcggtcgt
     7501 ggttgcgggc ggctcgtttg ccatcgctca gtaccttagc tctaacttca ttgggccgga
     7561 gctgccggac attatctctt cgctggtatc actgctctgc ctgacgctgt tcctcaaacg
     7621 ctggcagcca gtgcgcgtat tccgttttgg tgatttgggg gcgtcacagg ttgatatgac
     7681 gctagcccac accggttaca ctgcgggtca ggtgttacgt gcctggacac cgttcctgtt
     7741 cctgacagct accgtaacac tgtggagtat cccgccgttt aaagccctgt tcgcatcggg
     7801 tggcgcgctg tatgagtggg tgatcaacat tccggtgccg tacctcgata aactggttgc
     7861 ccgtatgccg ccagtggtca gcgaggctac agcctatgcc gccgtgttta agtttgactg
     7921 gttctctgcc accggcaccg ccattctgtt tgctgcactg ctctcgattg tctggctgaa
     7981 gatgaaaccg tctgacgcta tcagcacctt cggcagcacg ctgaaagaac tggctctacc
     8041 catctactcc atcggcatgg tgctggcgtt cgcctttatc tcgaactatt ccggactgtc
     8101 atcaacactg gcactggcac tggcgcacac cggtcatgca ttcaccttct tctcgccgtt
     8161 cctcggctgg ctgggggtat tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc
     8221 cgcgctgcaa gccaccgcag cacaacaaat tggcgtctct gatctgttgc tggttgccgc
     8281 caataccacc ggtggcgtca ccggtaagat gatctccccg caatctatcg ctatcgcctg
     8341 tgcggcggta ggcctggtgg gcaaagagtc tgatttgttc cgctttactg tcaaacacag
     8401 cctgatcttc acctgtatgg tgggcgtgat caccacgctt caggcttatg tcttaacgtg
     8461 gatgattcct taatgattgt tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg
     8521 cgggcgctga ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc tgagcgccaa
     8581 ctggcgatgc aactcggcgt atcacgtaat tcactacgcg aggcgctggc aaaactggtg
     8641 agcgaaggcg tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac
     8701 acatggtcgg agcaaaacat cgtccagccg ctaaaaacgc tgatggccga tgatccggat
     8761 tacagtttcg atattctgga agcccgctac gccattgaag ccagcaccgc atggcatgcg
     8821 gcaatgcgcg ccacacctgg cgacaaagaa aagattcagc tttgctttga agcaacgcta
     8881 agtgaagacc cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa
     8941 gcctcacata acatcgtgct gctgcaaacc atacgcggtt tcttcgatgt cctgcaatcc
     9001 tcagtgaagc atagccgtca gcggatgtat ctggtgccac cggttttttc acaactgacc
     9061 gaacaacatc aggctgtcat tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa
     9121 gcaatgatgg cgcaccttag ttttgttcac accaccatga aacgattcga tgaagatcag
     9181 gctcgccacg cacggattac ccgcctgccc ggtgagcata atgagcattc gagggagaaa
     9241 aacgcatgat tatttccgca gccagcgatt atcgcgccgc agcgcaacgc attctgccgc
     9301 cgttcctgtt ccactatatg gatggtggtg catattctga atacacgctg cgccgcaacg
     9361 tggaagattt gtcagaagtg gcactgcgcc agcgtattct gaaaaacatg tctgacttaa
     9421 gcctggaaac gacgctgttt aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg
     9481 gtttgtgtgg catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc
     9541 atggcattcc gtttactctc tcaacggttt ccgtttgccc gattgaagaa gtcgcgccag
     9601 ccatcaagcg cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc tttatgcgta
     9661 acgcgctgga gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc gtggatatgc
     9721 cgacaccggg agcgcgttat cgtgatgcgc attctgggat gagcggccca aatgcggcaa
     9781 tgcgccgcta cttgcaagcg gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg
     9841 gtcgtccgca tgatttaggt aatatttcgg cttacctcgg caaaccgacc ggactggaag
     9901 attacatcgg ctggctgggg aataacttcg atccgtccat ctcatggaaa gaccttgagt
     9961 ggatccgcga tttctgggat ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg
    10021 cgcgcgatgc agtacgtttt ggtgctgatg ggattgtagt ttctaaccac ggtggccgcc
    10081 agctggacgg tgtactctct tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag
    10141 gtgatatcgc cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga
    10201 ttgcgctcgg tgccgacacc gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag
    10261 cgggccaggc gggtgtagct aacctgctaa atctgatcga gaaagagatg aaagtggcaa
    10321 tgacgctgac tggcgcgaaa tcgatcagtg aaattacgca agattcgctg gtgcaggggc
    10381 tgggtaaaga gttgcctacg gcactggctc cgatggcgaa agggaatgcg gcatagtcgt
    10441 ttgagccccc tcaccctaac cctctccctc agggagtggg gaccgttcgg cgctgtatgt
    10501 actccctcac tctgaaacga caccgcactc tttttttctc cctcgcccct ccggggtgag
    10561 gggaaaaggc cgcactgctc accaattttc tgctatcctg cccccgcact aagggggcag
    10621 tatgctaaac atcgtacttt acgaaccaga aattccgcca aatactggca atatcatccg
    10681 tctttgcgct aataccggct ttcgtctgca tatcatcgaa ccgatgggat ttgcctggga
    10741 cgataagcgc ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca
    10801 tcatgacttt cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac
    10861 gaaaggtacg cctgctcaca gcgccgtaaa ctatcaggat ggcgattatc tgatgttcgg
    10921 cccggaaaca cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat
    10981 tcgcattccg atggtgccgg acagccgcag catgaacctg tccaatgcgg tgtcagttgt
    11041 ggtgtatgaa gcctggcggc agctgggata tccgggagcg ttgttgagag attagttact
    11101 gtatgccgga tagatgatta catcgcatcc ggcacgatca caggacatta gatcccatcc
    11161 ccatactcaa atgtatggtt aataccgttg aaatgctggt ccatatccat tgatggctta
    11221 tcgctgtctg gtttaccgac aatacgagcc ggaacgccag cggcggttgt atgcggcggc
    11281 accggttgca gcaccacgga acctgcgcca atcttcgcgc cgcgaccaac ttcaatattg
    11341 ccgaggattt tcgcgcccgc gccaatcatc acaccttcac gaattttcgg gtgacggtca
    11401 ccaccagatt tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca
    11461 attaccgccg tttcacccac aacgatgccc gtggcgtggt cgagcataat gccacggcca
    11521 atttttgctg ccgggtgaat atcgacctgg aacgtcacag aaacctggtt ttgcagaaag
    11581 attgccaatg cgcgacgccc ctgattccac aaccagtgac cgatgcgata ggcctgcaag
    11641 gcatgaaaac ccttcaggta taacaacggg gttgagtatt tatcgactgc cgggtcgcgg
    11701 gtacgcaccg cctgaatatc acaggccgca gaggcgatca tttccgggtc agcggcgtag
    11761 gcttcttcca ccacttcacg gatagcaata gcaggcataa ttggcgatga cagcttgttc
    11821 gccagcatgt agctcagtgc actaccaagg ttttcgtgct tgagtagcgt cgcgtggtaa
    11881 aaactggcca gcattggctc acagtccgcc agcgttctgg cttcggcttt aatattgttc
    11941 cagacaattt ccagttcttc acacgacatt gcttactcca cacgatgaga taatgaccgg
    12001 cccgttctgc gcgggccggg tcatagcggt aacaaaggtt ccctggggtt agtggctgct
    12061 gcgctcgtcc ttgcgtgcac gacctaataa cgtcaatgct gcctcgcgcg cgttttttcc
    12121 gcaatataat acttgataaa tttcctcggt tattggcatt tctacgccga agcgatgcgc
    12181 cagttcgcgg acttctttcg tattgcggta gccttccacc acctgaccaa tcttctcctg
    12241 cgcgctttgt acatccatgc cctgaccgag catcatgcca aaacggcggt tacgcgactg
    12301 gttgtcggta caagtaagca caagatcgcc cagccccgcc atgcccataa aggtggcagg
    12361 atcggcaccc agcgccgcgc caagacgcga catttcagcc agcccacggg tgatcagtgc
    12421 cgtacgcgca ttcgcaccaa aaccgatacc gtcggacatc cccgcgccaa tggcaatgac
    12481 gtttttcacc gcaccgccaa gctgcacgcc aatgaaatcc ggattgctgt aaacgcggaa
    12541 acttttgccg cagtgcagca gctgctggag atcatcggca aaggtctgat cggtcgaggc
    12601 cagcgaaata gctgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat
    12661 aaccgccagc ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc
    12721 ggtttccgct tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg
    12781 tttaatctgg cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg
    12841 gctggctgcc agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac
    12901 atcggggaga aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc
    12961 atggccccag aggacaacct cgtggccatt tcttgccagg gtgatggcaa gagcggtgcc
    13021 gtacgagccg gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga
    13081 tgttcttcag taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag
    13141 ttaaccggcg caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac
    13201 tcacgagcat acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg
    13261 gtgccttcga tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg
    13321 gtttcttcgc ccagggaggc cgttacggta acacgcagta ccacttcgta tacgtcatct
    13381 gccagttggg aagatgccgt atccagatca agtttaactt ctggttgcca atctttctgg
    13441 aaaacgtgcg gcgcgttcgg cgcttcgaaa gagatatcct tggtataaat acgttggatc
    13501 tggaaagtca tttcagtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc
    13561 cttaaatact gcgcaatgta atgccaacgt tcacacgtta tttcagcagg ggatccagtc
    13621 caccacgtgc atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa
    13681 aaatctgggg aaccgtggtg cgaccgctgc gtttgatcat ctcttcacgc ttggcggcat
    13741 tgccatcgat cggcaactcc tgaaaactca cgcccttgct gctcagcagt gcttttgcac
    13801 gatggcaata cgggcaggtt tctttggtat agatttcaac attggccata atttagctcc
    13861 tgttttttta ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa
    13921 gcttataaca ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac
    13981 gacaggtaaa ttatttgccg cgcaccaaag gcaagttttc gccagcccag ccagcgacgc
    14041 cttctttcag cacgaatact tgcgcaaaac cagctttcgt cagtgcgttt gcaggctcct
    14101 ggcactgcat gccagaaccg tctaccacga taaccggttt gtctttgtgc ttctcaagct
    14161 caccaacatt gttggctttg atttcgctcg gcaacagatt gatagaacct gcgatatggc
    14221 ctttacggaa gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct ttgttgatca
    14281 gacgcgtagc ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag
    14341 tcacaagaac cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa
    14401 caaattgcat aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc
    14461 aggaaaggag tatacctgcg cggtgcggca aatacagcca gcgcgttaac tggaatgcaa
    14521 ttttgcgggg cacgacgaaa aaaaagcgca aatctggcat cggcttgccg cgagcggtcg
    14581 tatattttga tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt
    14641 tgactcttga gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga
    14701 ttctggatgg ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa
    14761 ccccggtaat ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc
    14821 tggaagtcgg tctgcctgac cgtcagatgg gtaactccga agtaggccac gttaacctgg
    14881 gtgccggccg catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg
    14941 ctttctttgc taatccagtg ctgactggcg cggtagataa agcgaaaaac gcaggtaaag
    15001 cagtacacat tatgggtctg ctctccgcag gcggcgtaca cagccacgaa gatcacatca
    15061 tggcgatggt agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc
    15121 ttgacggtcg cgacaccccg ccgcgcagtg ctgaatcctc gctgaaaaaa tttgaagaga
    15181 aatttgctgc gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg
    15241 accgcgataa ccgttgggat cgcgttgaaa aagcctatga cctgctgact ctggcgcagg
    15301 gtgagttcca ggccgatact gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa
    15361 acgacgagtt cgtgaaagcg accgttattc gtgcagaagg tcagccagat gcggcaatgg
    15421 aagacggcga tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgc gaaatcactc
    15481 gtgcattcgt aaacgcagat ttcgacggct tcgcgcgtaa gaaagtggtt aacgtcgatt
    15541 tcgtgatgct gaccgaatac gccgctgaca tcaaaactgc ggttgcttac ccacccgctt
    15601 ccctggttaa caccttcggc gagtggatgg cgaaaaacga caaaactcag ttgcgtattt
    15661 ccgaaaccga aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt
    15721 tcaaaggcga agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac
    15781 cggaaatgag ctccgcagag ctgaccgaaa aactggttgc ggccatcaag agcggcaaat
    15841 acgacaccat tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggggtgatgg
    15901 aagcggcggt taaagcggtt gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg
    15961 ttgagtccgt gggtggacaa ctgctgatca ccgctgacca cggtaacgct gaacagatgc
    16021 gcgatccggc aacgggtcag gcacacacgg cacacaccaa cctgccagtt ccgctgattt
    16081 acgttggtga taagaacgtg aaagcggttg caggcggcaa actttctgac atcgcgccga
    16141 ccatgttgtc gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca
    16201 tcgtggaata atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga
    16261 aaccgcgcag gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat
    16321 tgttgtgcgc cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg
    16381 atatcgccgc aaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg
    16441 cacaattgaa aaagcaggaa gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc
    16501 aaaacacgct caatcaactc aataaacaga ttgatgagat gaacgcgtcg attgccaaac
    16561 tggagcagca aaaagccgcc caggagcgca gcctcgccgc acaactggat gccgcattcc
    16621 gtcagggcga gcataccggt attcagctga ttctcagcgg tgaagaaagc cagcgtggac
    16681 agcgtttaca ggcttatttc ggctatctca accaggcgcg acaagaaacc attgcccagt
    16741 tgaagcaaac gcgtgaagaa gtcgccatgc agcgtgctga actggaagag aaacagagcg
    16801 agcaacaaac gctgttatat gagcagcgcg cccaacaggc gaaactgact caggcgctga
    16861 acgagcgtaa aaagacgctg gcagggctgg agtcttccat ccagcaaggt cagcaacagt
    16921 tgagcgagct gcgcgccaac gaatcccgtc tgcgtaacag cattgcccgt gcggaagccg
    16981 cggcgaaagc gcgtgcagaa cgagaagcac gtgaggccca ggcggttcgc gaccgccaga
    17041 aagaagcgac gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt
    17101 cccgaactgg tggcctgggg gcgccgcgtg gtcaggcatt ctggccggtt cgcgggccga
    17161 cgctgcatcg ctatggtgaa cagctacagg gcgaactacg ctggaaagga atggttatcg
    17221 gtgcctctga aggtactgaa gttaaagcga ttgccgatgg tcgggtgatt ctggctgact
    17281 ggctgcaagg ttacggtctg gtggtggtgg ttgagcacgg taaaggcgac atgagtcttt
    17341 acggctataa tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccaa
    17401 ttgcactggt gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc
    17461 gccaaggtca ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc
    17521 gtcgtaacgt tcttgcattt gccgctctgt tggcgctctc ctcccccgta cttgctggca
    17581 aacttgccat cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg
    17641 cgatgccctc cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg
    17701 cgaccaaagc gcataacagc gggcacgaag tgttgattca tctcccgatg gcaccactga
    17761 gtaaacagcc gctggagaaa aatacgctgc gcccggagat gagcagcgac gaaattgagc
    17821 gcattattcg tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg
    17881 gtagcaagat gacctctaac ctgtttggta tgcagaaagt gatgcaggca ctggagcgtt
    17941 acaatcttta cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgccgcgc
    18001 aaggtactgg cgtgaaggtg atcaaacgga aggtgttcct cgacgattcg cagaacgaag
    18061 cggacatccg tgtgcaattt aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca
    18121 ttgccattgg gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc
    18181 tgccgccaga cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata
    18241 cttctacacc gccgaaaaac gctgtgcctg atgcaccgcg taatccgttc cgtggcgtga
    18301 agctgtgcaa accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat
    18361 taagcgaaag catcagccag agcacgctga tcgtttactt ccagcatcag tggcaaggct
    18421 ggggcaaaca gcccgaagcg gcgaagttta acgctagcgc aaattaaagg cgctacgcgt
    18481 aatgcgatgg tgcgaccgcg tttttttcgc gccattgcca taagcggaaa gaccacagta
    18541 acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta tacataccgg
    18601 aagtaaatat ctcggcaatc atccgttggc gggtaagaat atccggctct ttgcgcaccg
    18661 catggcaaac acgcaatgct tcgtaagtta tttgctgatg aaattccgga taaatcataa
    18721 ttttgtcggc ataatttcga tttaatttct ccagcaggcg ggtaatctta atatagtgac
    18781 gttgataatt aaggttttta ttcccttgtc tatgcaaccg actcactgac gtattatgca
    18841 gataatattt atataatgat tgctcggtat atcgcgcacg cagcgcgtta aacatgaatt
    18901 ctgttgtcca gacaatatcc tgatgatgta atccggcaat aaatttaatg ttatttttaa
    18961 caataacatc acggcgataa acccccatcc agacaacgtg agtccaacgg cgcgaagaaa
    19021 gccccatccg cagccagtcc gggccggtta atacaccggt tgagcgaagg cgatcgctgg
    19081 ggatggattg ccaggtttct cccgtttcac gaaaagacca gtcagcgttg cactgcgcca
    19141 cgtcgaggtc gtcctctaac gccatggtca tcagcgtttc gtacatggtg ggatagacct
    19201 catcgtcagc atcgacaaaa gcgacatatt tgcccgtcgc cacctcgatc ccacgattac
    19261 gcgctaccga tgcgccagca ttcgcctgat gcaacaaacg aacgtgcgga tagttttctg
    19321 cgtaatgctt tgctatttca acagaattat ccgttgaacc atcgttaata ataatgattt
    19381 ccagagcagt ccaggtttgc gtaattaaag attccataca agtgcggaaa tcatcgcccg
    19441 cattatataa cggaataata acactaagtt tattggtgct gttcatcata aattaccgac
    19501 gacctctgaa gttatcctga cgttttacag gctttgtatt aagaaactat taagcctgtg
    19561 agatattatt tttacaggtg agagagtcac ttcagggtag agagataatg agaacagcta
    19621 tataaaagat cagcgcaatt acgaactctt tattacactg acaggatttt acatatttaa
    19681 cagattatcc tcaggcaaaa gggctggaat accagccctt tttcgtgtta atcccagctc
    19741 agaataactt tcccggactg gcccgaacac atagcgtcaa agcccttctg gaaatcatcg
    19801 atagagaaac gatgggtaat gatcggcgag agatcgaggc cagactgaat cagcgccgcc
    19861 atcttgtacc aggtttcaaa catctcacga ccgtaaatgc ctttaatgaa caagccttta
    19921 aagatcactt tggtccagtc gatagacatg tcagacggtg gaatacccag catcgcaata
    19981 cgaccgccgt ggttcatggt atcgagcatg gtacgaaacg ctggcggcgc gccggacatt
    20041 tccagaccga catcaaagcc ttcggtcatg cccagttcag ccatcacatc attaaggttt
    20101 tctttcgcga cgttaaccgc acgggtgata cccattttgc gcgccagctc aaggcggtat
    20161 tcgttaacat cggtgatcac cacattgcgt gcaccaacgt gtttcgccac cgccgcagcc
    20221 ataataccta tcggacctgc accagaaacc agcacatctt cgcccaccag atcgaacgac
    20281 agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc gtcggagata
    20341 ttgtcgggga ttttgaaagc gttgaacgcc ggaatcacca gatattcggc gaagcagccc
    20401 ggacggttaa caccgacacc aatggtgttg cggcacagat gggtacgacc gccacgacag
    20461 ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc gattttgaag
    20521 cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc tacgaccatc
    20581 ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac gtcagtcccg
    20641 cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc cggtacagga
    20701 acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt catctcacat
    20761 cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac tgcacgcgta
    20821 atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg acctttcgga
    20881 acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc acgggcaaat
    20941 ttctgcgcca ctactgcatc gccaagcatg accggaataa tggcatgatc ggctcccgcc
    21001 agggtaaagc ccgccgccga catttgctca cggaactggc gcgcgttcgc ccacagacgg
    21061 tcacgcagtt cgctgcccgc ttcgaccatc tccagtactt tgatggacgc ggcaacaatg
    21121 gccggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag ccactcaacc
    21181 acctctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt accaagcgta
    21241 ccggtgataa tatcgacccg gcccatcaca tcgcagtatt catgggaacc acgaccattt
    21301 tcaccgacaa aaccgaccgc gtgggagtca tccaccatca ccagggcatc gtatttatct
    21361 gccagatcgc aaacgccttt caggttggca atcacgccgt ccattgagaa cacaccatcg
    21421 gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg tgcttccagc
    21481 tcctgcatat cgttgttggc atagcgatag cgtttagctt tgcacagacg cacaccatca
    21541 ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcacc cagcagcgtt
    21601 tcaaacagtc caccgttagc atcaaagcag gaagagtaga gaatcgcatc ttccatcccc
    21661 aggaatgccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc gcaaataaaa
    21721 cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgctgc cgcaatcaga
    21781 tccggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac gtggcttcca
    21841 tcagccacag tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc tttaaacaac
    21901 ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc tccacgcatt
    21961 gcgattctcc agacagggca aattccagca catattaccc aaacttatag gtcgcgacga
    22021 gatgacgcgt taactcttct gcaaaattca ggataaatga cgtgtaactt gatgtctctc
    22081 aacacctaca gatagtagaa aaactgtaca gttttggctg ggcataatga tgattgattt
    22141 tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct atctcagttg
    22201 gtatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga aaattctcag
    22261 cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat cgaaccattt
    22321 tcctctcccc agatagttta ggttgaagag atcggggcgt ttgtaatagc acatgacaaa
    22381 aatgccctgg tcatcatcca caatgttatt atttagtgtt attttttgac tttccagaac
    22441 taatttatag aattctttcc atttatgctg tgaaccaaca attgcgcccc caataatata
    22501 tacatgattg ccaatcatga aatcaaaaac ctgttgttga gatgtgacag taagcccttt
    22561 ttttatagtg aaaagatgca tcttactttc atcaaatggg aaatcccaaa ttttcaatcc
    22621 tcgagttaca tttggcttac ggcaatagcc aaaatctatc cacgcaacaa gaggcgtttt
    22681 cactaagccc atgttgatag ctttatttac aaaataggcc ttaaggttac ataccaaaac
    22741 atactctggt gaccagtact ctgggttttt taattgtcga ggctcaagtc tatttgtaaa
    22801 cgactcatct ttttgaattt tttcgatacg acttctgata tatctaaatt tcttttttat
    22861 atcgataacg ataaccgttg ttggttttcc gttgcgaatc gcttcaaccc ttggtttcaa
    22921 atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct caaagtaact
    22981 aaaataaaca tcaactgaac gagctagttt ttcacggaac cctttattag cagtccaatc
    23041 ccctcta
//
LOCUS       DABSYT020000070        22953 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.070, whole genome shotgun sequence.
ACCESSION   DABSYT020000070 DABSYT020000000
VERSION     DABSYT020000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.070"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            77..1213
                     /locus_tag="ID725_RS16170"
                     /old_locus_tag="pgaptmp_003234"
                     /pseudo
     CDS             77..1213
                     /locus_tag="ID725_RS16170"
                     /old_locus_tag="pgaptmp_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            1207..1344
                     /locus_tag="ID725_RS16175"
                     /old_locus_tag="pgaptmp_003235"
                     /pseudo
     CDS             1207..1344
                     /locus_tag="ID725_RS16175"
                     /old_locus_tag="pgaptmp_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
     gene            complement(1346..1957)
                     /gene="emtA"
                     /locus_tag="ID725_RS16180"
                     /old_locus_tag="pgaptmp_003236"
     CDS             complement(1346..1957)
                     /gene="emtA"
                     /locus_tag="ID725_RS16180"
                     /old_locus_tag="pgaptmp_003236"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGV7851771.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            2057..2971
                     /gene="ldcA"
                     /locus_tag="ID725_RS16185"
                     /old_locus_tag="pgaptmp_003237"
     CDS             2057..2971
                     /gene="ldcA"
                     /locus_tag="ID725_RS16185"
                     /old_locus_tag="pgaptmp_003237"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGV7851772.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            3067..4803
                     /gene="cvrA"
                     /locus_tag="ID725_RS16190"
                     /old_locus_tag="pgaptmp_003238"
     CDS             3067..4803
                     /gene="cvrA"
                     /locus_tag="ID725_RS16190"
                     /old_locus_tag="pgaptmp_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGV7851773.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(5192..6262)
                     /gene="dadX"
                     /locus_tag="ID725_RS16195"
                     /old_locus_tag="pgaptmp_003239"
     CDS             complement(5192..6262)
                     /gene="dadX"
                     /locus_tag="ID725_RS16195"
                     /old_locus_tag="pgaptmp_003239"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGV7851774.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(6272..7570)
                     /gene="dadA"
                     /locus_tag="ID725_RS16200"
                     /old_locus_tag="pgaptmp_003240"
     CDS             complement(6272..7570)
                     /gene="dadA"
                     /locus_tag="ID725_RS16200"
                     /old_locus_tag="pgaptmp_003240"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGV7851775.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7933..9465
                     /gene="ycgB"
                     /locus_tag="ID725_RS16205"
                     /old_locus_tag="pgaptmp_003241"
     CDS             7933..9465
                     /gene="ycgB"
                     /locus_tag="ID725_RS16205"
                     /old_locus_tag="pgaptmp_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGV7851776.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(9517..10236)
                     /gene="fadR"
                     /locus_tag="ID725_RS16210"
                     /old_locus_tag="pgaptmp_003242"
     CDS             complement(9517..10236)
                     /gene="fadR"
                     /locus_tag="ID725_RS16210"
                     /old_locus_tag="pgaptmp_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGV7851777.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            10458..11999
                     /gene="nhaB"
                     /locus_tag="ID725_RS16215"
                     /old_locus_tag="pgaptmp_003243"
     CDS             10458..11999
                     /gene="nhaB"
                     /locus_tag="ID725_RS16215"
                     /old_locus_tag="pgaptmp_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGV7851778.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            12145..12675
                     /gene="dsbB"
                     /locus_tag="ID725_RS16220"
                     /old_locus_tag="pgaptmp_003244"
     CDS             12145..12675
                     /gene="dsbB"
                     /locus_tag="ID725_RS16220"
                     /old_locus_tag="pgaptmp_003244"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGV7851779.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(12721..13989)
                     /gene="umuC"
                     /locus_tag="ID725_RS16225"
                     /old_locus_tag="pgaptmp_003245"
     CDS             complement(12721..13989)
                     /gene="umuC"
                     /locus_tag="ID725_RS16225"
                     /old_locus_tag="pgaptmp_003245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGV7851780.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(13989..14408)
                     /gene="umuD"
                     /locus_tag="ID725_RS16230"
                     /old_locus_tag="pgaptmp_003246"
     CDS             complement(13989..14408)
                     /gene="umuD"
                     /locus_tag="ID725_RS16230"
                     /old_locus_tag="pgaptmp_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGV7851781.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            14781..15692
                     /gene="hlyE"
                     /locus_tag="ID725_RS16235"
                     /old_locus_tag="pgaptmp_003247"
     CDS             14781..15692
                     /gene="hlyE"
                     /locus_tag="ID725_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HGV7851782.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGNIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AANKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(15899..16345)
                     /gene="ycgN"
                     /locus_tag="ID725_RS16240"
                     /old_locus_tag="pgaptmp_003248"
     CDS             complement(15899..16345)
                     /gene="ycgN"
                     /locus_tag="ID725_RS16240"
                     /old_locus_tag="pgaptmp_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGV7851783.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(16437..17096)
                     /gene="ycgM"
                     /locus_tag="ID725_RS16245"
                     /old_locus_tag="pgaptmp_003249"
     CDS             complement(16437..17096)
                     /gene="ycgM"
                     /locus_tag="ID725_RS16245"
                     /old_locus_tag="pgaptmp_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGV7851784.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(17168..17461)
                     /locus_tag="ID725_RS16250"
                     /old_locus_tag="pgaptmp_003250"
     CDS             complement(17168..17461)
                     /locus_tag="ID725_RS16250"
                     /old_locus_tag="pgaptmp_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGV7851785.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(17473..17631)
                     /locus_tag="ID725_RS16255"
                     /old_locus_tag="pgaptmp_003251"
     CDS             complement(17473..17631)
                     /locus_tag="ID725_RS16255"
                     /old_locus_tag="pgaptmp_003251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851786.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            17702..18103
                     /gene="pliG"
                     /locus_tag="ID725_RS16260"
                     /old_locus_tag="pgaptmp_003252"
     CDS             17702..18103
                     /gene="pliG"
                     /locus_tag="ID725_RS16260"
                     /old_locus_tag="pgaptmp_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGV7851787.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(18206..18574)
                     /gene="ycgJ"
                     /locus_tag="ID725_RS16265"
                     /old_locus_tag="pgaptmp_003253"
     CDS             complement(18206..18574)
                     /gene="ycgJ"
                     /locus_tag="ID725_RS16265"
                     /old_locus_tag="pgaptmp_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGV7851788.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            19094..19789
                     /gene="minC"
                     /locus_tag="ID725_RS16270"
                     /old_locus_tag="pgaptmp_003254"
     CDS             19094..19789
                     /gene="minC"
                     /locus_tag="ID725_RS16270"
                     /old_locus_tag="pgaptmp_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGV7851789.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            19813..20625
                     /gene="minD"
                     /locus_tag="ID725_RS16275"
                     /old_locus_tag="pgaptmp_003255"
     CDS             19813..20625
                     /gene="minD"
                     /locus_tag="ID725_RS16275"
                     /old_locus_tag="pgaptmp_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGV7851790.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            20629..20895
                     /gene="minE"
                     /locus_tag="ID725_RS16280"
                     /old_locus_tag="pgaptmp_003256"
     CDS             20629..20895
                     /gene="minE"
                     /locus_tag="ID725_RS16280"
                     /old_locus_tag="pgaptmp_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGV7851791.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(21267..22013)
                     /locus_tag="ID725_RS16285"
                     /old_locus_tag="pgaptmp_003257"
                     /pseudo
     CDS             complement(21267..22013)
                     /locus_tag="ID725_RS16285"
                     /old_locus_tag="pgaptmp_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            22017..22109
                     /locus_tag="ID725_RS16290"
                     /old_locus_tag="pgaptmp_003258"
                     /pseudo
     CDS             22017..22109
                     /locus_tag="ID725_RS16290"
                     /old_locus_tag="pgaptmp_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(22134..22718)
                     /locus_tag="ID725_RS16295"
                     /old_locus_tag="pgaptmp_003259"
     CDS             complement(22134..22718)
                     /locus_tag="ID725_RS16295"
                     /old_locus_tag="pgaptmp_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGV7851792.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
BASE COUNT         5790 a         5789 c         5477 g         5897 t
ORIGIN      
        1 agaggatatt attcattgac ttaaacaaaa tgacaaaatc catagaccca tagcctgggt
       61 actatatagg agtggtatgg gcatcaaaca acacaatggg aataccaaag ccgatcgtct
      121 cgctgaatta aaaatccgtt cgccctcaat tcaactgata aaatttggcg ctattggttt
      181 gaatgcaatt ctcttttccc ccctgctgat agctgctgat acaggaagtc aatatggcac
      241 caatattact attaatgatg gtgacagaat tactggagat accgccgatc catcaggaaa
      301 cctctatggt gtaatgaccc cagcaggaaa cacgcctggc aatatcaacc tgggtaatga
      361 tgtcaccgtc aatgtcaacg acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa
      421 aaacagctcc ctgacagcta accgactcac agtagatgtt gttggtcaaa cctctgccat
      481 cggcattaat ttaattggtg actataccca tgctgactta ggcacaggca gcaccattaa
      541 gagtaacgat gacggcatca ttattgggca tagctcaaca ctaacagcca ctcaattcac
      601 cattgaaaac tcgaacggta taggcctaac catcaatgac tatggcacca gtgtcgatct
      661 tggaagcgga agtaaaatca agaccgatgg aagtacaggt gtttatatcg gtggtctcaa
      721 cggcaataac gccaatggtg ctgcgcgttt tacggcgaca gacctgacaa tcgatgttca
      781 gggctacagc gccatgggga taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa
      841 cagttccatt aaaaccagtg gcgataatgc acacggcctc tggagctttg gccaggttag
      901 cgcgaatgca ctcactgttg atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg
      961 tggtacaacc actatcggtg cagatagcca tatttcttcc gcgcagggcg gtggtctcgt
     1021 caccagtggt tcagacgcga caatcaattt ttctggcacg gcagcgcaac gaaacagcat
     1081 cttttccggc ggttcttatg gtgcctcggc ccagacggca acggctgtta tcaacatgca
     1141 aaataccgat attacggttg atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag
     1201 cggcgcaaga atgaaaccat caccactccc cgtctgagct tccgttttct taacgtcagc
     1261 ccgacggtgg agcggcaatt acagcggatt attttctctc tcgagcggga agcccgggag
     1321 aaagcggaca aagtacgcga ctgatttaca tcgcgtccag tgcctgctca agtttgtaga
     1381 tatagcgcgg agcctgcggc gcaggatgat ttcgcgctac gtgatcgagg aactcgtcag
     1441 catccagatc gttgattttg ctgattgcct ttttccgatc tgacgagaaa gtccgtagca
     1501 gcgcacctgc cccgttagca tatgacacca ccagcgcata ttgcagtacc ttcggatctt
     1561 caatgcctgc cagcgggccg gtttccagaa tattcaggta agccgccccc atagaaatat
     1621 tacgctccgg atttttcagc tcgctggtcg tcggctcacc actccagccc atacgccgat
     1681 aaacatcacg tccggaggtt gaagctttta actgcatcaa accaatggca ttcgatttac
     1741 tcaccgcgtt cggattacca cccgattcga tagcgataat cgccgtgatc aattgtggat
     1801 cgacgcccca ggctgcaccg gctttctggc ttattggcat ccactgcatc gcacgttgca
     1861 ccggaacttt cgcgttccac ggcgggttcg tatagtcatg ctttgatgaa caacccgcta
     1921 ataacacaat caaaaaggca aaccatctca atttcacttc ttctatcctt atagcctgga
     1981 atcgccgttg aggtgacaac ggcacaccta agcggcatga tatacatttc gtatctgttg
     2041 tcagcaagga attgccatgt ctctgtttca cttaattgcc ccatcgggtt actgcattaa
     2101 acagcacgcc gcgctgcgtg gtatccagcg ccttaccgac gcggggcatc aagtcaataa
     2161 cgtcgaggtt attgcccgtc gttgtgagcg ttttgcgggt acggaaacgg agcgtctgga
     2221 agatctcaat tcccttgcca gactgactac ccccaacacc atcgtgctgg ctgtacgcgg
     2281 cggttacggt gccagtcgtt tactggcaga tattgactgg caggcactgg tggctcgcca
     2341 acaatatgac ccgttgctca tatgcgggca tagcgatttt accgccattc agtgcggtct
     2401 tctggcgcaa ggcaatgtca tcacctttag cggccccatg ctggtagcaa actttggcgc
     2461 ggatgaactg aacaccttta ccgagcacca tttctggctg gcgttacgca atgaaacctt
     2521 taccattgaa tggcaagggg aaggcccgac atgtcgggcc gaaggcacgt tgtggggagg
     2581 caatcttgcg atgctgattt cactgatcgg tacaccgtgg atgccaaaaa ttgagaacgg
     2641 tattctggtg ctggaagata ttaacgagca ccctttccgc gtcgaacgta tgctgttgca
     2701 gctctatcat gcgggaattt tgccacgtca gaaggcgatt attctcggta gctttagcgg
     2761 cagtacgcct aatgattatg acgcaggtta caacctggag tcagtgtacg catttttgcg
     2821 ttcccgcctg tcgattccac tcattaccgg cctcgatttt ggtcatgaac aacgcacggt
     2881 tacgctgccg ttgggcgcac atgccattct tactaacacc cgggaaggca ctcagttgac
     2941 gattagcggt catcctgttc ttaaaatgta aaaaaaatca cgcttcaggg ctaagtaaaa
     3001 cgctgtctct gccgctaata tggtagggtt tatataagag acagcgtaat caggagtaac
     3061 cgaccgttgg atgccacaac aataattagc cttttcatct taggatccat ccttgttacc
     3121 agcagtatat tacttagttc attttcttct cgtcttggca ttcctattct ggttattttt
     3181 ctggcgatcg gcatgctggc gggagtcgat ggcgtcggcg gtatcccatt tgataattac
     3241 cccttcgcct acatggtaag taacctggcg ctggcgatta ttttgctcga tggcgggatg
     3301 cgcactcagg ccagttcctt tcgtgtggcg ttaggaccgg cgctgtcgct ggcgacgctg
     3361 ggcgtactta tcacctctgg tttaaccggc atgatggcgg cgtggctgtt taatcttgat
     3421 ttgattgaag gcttattaat cggcgctatc gtcggctcca ccgatgctgc agcggtcttt
     3481 tctctgctgg gtggtaaggg gcttaacgaa cgtgttggct cgacgctgga aattgaatcc
     3541 ggcagtaatg atccaatggc ggtctttctg acgattaccc tgattgcgat gatccagcag
     3601 catgaaagca gtgtcagttg gatgttcgtg gtcgatattc tgcagcaatt tggcctcggg
     3661 attgtcatcg gtcttggcgg tggttattta ctgctgcaaa tgattaatcg catcgccctg
     3721 cccgccggtt tatatccatt gctggcatta agcggcggta ttttaatttt tgctttaacc
     3781 acggcgctgg aaggtagcgg tattctggct gtttatctgt gcggttttct gctgggtaat
     3841 cggccgattc gcaaccgcta cggcatcctg caaaatttcg acggcctcgc ctggctggcg
     3901 caaatcgcca tgttcctggt gctggggctg ttggttaacc caagcgatct gctgcctatt
     3961 gccattccgg cgctcatttt gtccgcatgg atgattttct tcgcccgtcc tctttcggta
     4021 tttgccggat tgctaccatt ccgtggcttc aatctgcgtg agcgcgtgtt tatcagctgg
     4081 gtgggattac gcggcgcggt gccgattatt ctggccgtgt tcccgatgat ggcggggctg
     4141 gagaatgcac gtctgttctt taatgtcgcc ttctttgtgg ttctggtttc actgctcttg
     4201 cagggaacat cactctcgtg ggcggcgaaa aaagccaaag tggtggttcc gcccgtcgga
     4261 cgtccggtgt cacgcgttgg cctggatatt catccggaaa atccgtggga gcagtttgtt
     4321 tatcaattga gtgccgataa atggtgcgtg ggcgcggcac tgcgcgattt gcatatgcca
     4381 aaagagacgc gtattgcggc actgtttcgt gataaccagt tgcttcatcc taccggcagc
     4441 acccgactgc gcgaaggcga tgtgttgtgt gtaattggtc gggaacgcga tctcccggcg
     4501 ctcggtaaac tgttcagcca gtcgccgccg gtcgcgctgg atcaacgctt ctttggtgac
     4561 ttcattctcg aagccagcgc caaatatgca gatgtggcgc tgatatatgg cctggaagac
     4621 gggcgagaat atcgtgataa gcaacaaacg ctgggtgaaa ttgtccagca gttgttaggc
     4681 gcggcaccgg ttgtcggtga ccaggtagag tttgccggga tgatctggac ggtggccgag
     4741 aaagaagata atgaagtgtt gaagattggc gttcgagtgg ctgaagagga agctgaatct
     4801 taaatttaat gaagcatcta tcgccggtta cgatgctttg cttaacgcag cctgagcctg
     4861 tatataaact tgtcatttcc tgattttgat atgcacattc caacatcaga agaagcctgt
     4921 taatcaacat tctgaacatg aacccgaatc tgcatcaagg gtgaagcgtg atttatctga
     4981 agtcgagttc gagtcaacaa tgaagcgtaa gcgttttatg tagaacgcct gtgcggtgag
     5041 ttcaggttcc ggggaaatag tgtccatcct tttaatgggc actatcatgg gcaatggctc
     5101 tgtcacatta acaactacag ttgctgacca gccggatgga tgattttttt tgcaaccaga
     5161 agacgttgcc tccgatccga cttacaacaa gttacaccgt cacaaccggg acgcgtaacg
     5221 ccagcgcgca catcaactca tagcccaccg ttccggcagc ggcggcgaca tcatcaattt
     5281 tgatctcctt gccccacagc tcaactggcg taccaatacc cgcctgcggg caaggcgtta
     5341 aatcgaccgc cagcatatcc atcgagactg tccccaccgt catggtgcgc acgccgtcca
     5401 ctaaaacagg ggtaccggta ggcgcgtggc gcggataacc gtcggcgtat cctgcggcaa
     5461 caatgccgat tcgctgctca tcgcgcgcag tataacgacc gccgtagccc acacgctcgc
     5521 ccgcttttag cgtctggaca ccaataatct cactgcttag cgtcatcacc ggacgtaatc
     5581 cagtattggc gatatcacgc cactgaccgg acggcgaagc gccatacaaa ataatgccag
     5641 gccgaaccca gtcaaaatgc gcttctgggt gccacagagt cgccgccgaa ttggacaacg
     5701 aacgccgaca ctccagccct tccgccgcct gctcaatacg cgccatcgcg ccggaaattc
     5761 catcaggatg ttccgcctcg gcaaaatgcg acatcagggt catttcgcca acattcgcca
     5821 tcgcccgcaa ctgctgccag acggtaagca cgcgatcggg ctggaagccc aaccgattca
     5881 tcccactgtt cactttaaga taaatatcca acggtgcttt tagccgcgca ttttgcagtg
     5941 ctttgagctg ccagttgctg tgtacgcagg tggtcaggcg gtgctggtca tacatttcca
     6001 gatcctgagc atggaaaaat ccttccagca tcaggatcgg tcctttccag ccgcgctcac
     6061 gtaacgttat tgcttcttcc aggttaagta atgcaaagcc atcggtagcc ccgagcgcgc
     6121 tccagatacg ctcaataccg tgcccgtaag cgttcgcttt taccaccgac cagacgcgcg
     6181 cgtgcggcgc ggcctggcgg acaatggaca gattctgttt taatgcctgc agatcgaggc
     6241 tggcctgtat cggacgggtc atctcgtttc cttagctgtg tgcgccatgt aaatggcccg
     6301 gacgtgatgg cgtaaatcca cggctgtagc gcgctacgct tagatcctca tatgggatcg
     6361 ctggcgtgcg accagagagc agatcgctta acaactgacc ggaaccgcaa gccatcgtcc
     6421 agccgagcgt gccgtgaccg gtatttagcc acagattttt aaagcgtgta cgcccgacaa
     6481 ccggcgtgcc gtctggcgtc atcgggcgca gacccgtcca gaaagtcgcc tgctcgacat
     6541 gaccgccgcg tggatagaga tcgcgaacca ccatctccag cgtttcacga cgcggttgca
     6601 acagctcggt attaaaacca acaatttccg ccattccgcc aacacgaatg cggttatcga
     6661 aacgggtaat ggcgattttg taggtttcat caagaatggt ggataccggc gcaccatctt
     6721 cctgcgcaat tggaatggtc agcgagtagc ctttcagcgg atacaccgga atatcaacaa
     6781 tgcctttgag catcgccgtc gagtaagaac caaacgccat cacatacgca tcggccttaa
     6841 tcacttcatc gccacacttc acgccgtaga tttgctcgcc gtcgcaaagc agttggtcaa
     6901 cgggcgtatt aaagcggaat ttaacccccg cctgctccgc catccgcgcc agattctggg
     6961 taaacagctg gcagtctccg gtttcatcat tgggcaattg cagaccaccc gtcagtttgt
     7021 gcgctacttc tgccagcgcg ggctccactt ccgccaggcg gctggattcc agcagctgat
     7081 acggtacgcc ggcatcttcc agcacggcga tatcgcgggt cgcattttca tactgttgtt
     7141 cggtacggaa cagttgcagc gtcccaccct gacgcccttc atactgaata ttggtttcgg
     7201 cgcgcaatgc tttcaggcaa tcacggctgt attccgccag acgcaccatc cgccctttgt
     7261 tttccatgta gtggctggtg tcgcagttac gtaacatttg ccacatccat ttcaactgga
     7321 actgcgtacc gtcgagacga accgccagcg gcgcatggcg ctggaacatc catttaatcg
     7381 cttttaaagg cacacctggt gccgcccacg gtgcagcata tccgggggag atttgcccgg
     7441 cattcgcggc actggtttcc agggcggccc ccggctcccg atcaatgacg gtgacctcat
     7501 gtcctgcctg atttaagtac caggcgctgg caacgcctac cacaccactt cccagtatga
     7561 caactcgcat agccactccg ataacggtag atacagtaaa agaataataa tctaattaca
     7621 tcttgataac tcagttgaaa atattattca acatacggct tttttatggt gagctggctc
     7681 acatctccct gactctgcgg caatatggcg tctttagtat ctcctgctga acgccctttt
     7741 tatccagcat aaaattccgt tcagaaacgg attagtggca ctctgataat aagaaatcgc
     7801 aaagaaaaaa attctccggc gacgcggttt ttaacaggtg ttctatgctt gaaatgaggt
     7861 gctccacaca gagagcgccg acaacaatga ggtgctccac acagagagcg ccgacaacaa
     7921 tgaggtgcgc gtatggcgac gatcgattct atgaataagg acaccacacg tttgagcgat
     7981 ggacccgact ggacgttcga cctgctggat gtttatctgg cagagataga ccgggtggcg
     8041 aaactctacc ggctggatac ctacccgcac cagattgaag tgataacctc agaacagatg
     8101 atggatgcct actccagcgt cggcatgcca attaactatc cgcactggtc attcggtaaa
     8161 aagtttatcg agactgaacg gctgtataag cacggtcagc aaggactggc ctatgaaatc
     8221 gtcattaact ctaacccgtg tatcgcttac ctgatggaag agaacaccat taccatgcaa
     8281 gcgctggtga tggctcatgc ctgctatggg cataactctt tcttcaaaaa caattactta
     8341 ttccgtagct ggaccgacgc cagttcgatt gtcgattatc tgatttttgc ccgtaaatat
     8401 attaccgagt gcgaagaacg ttatggcgtt gatgaagtag aacggcttct ggactcgtgc
     8461 cacgcgctga tgaactacgg cgtggaccgg tacaaacgcc cgcaaaaaat ctcgctgcaa
     8521 gaagagaaag cccggcagaa aagtcgcgaa gagtatttgc aaagtcaggt caatatgctc
     8581 tggcgtaccc tgccgaagcg cgaggaagag aaaacggttg ctgaagcgcg ccgttatccg
     8641 tccgaaccac aagaaaacct gctctatttt atggagaaaa atgcgccact gctggaatca
     8701 tggcagcgtg aaatcctgcg tattgtgcgt aaggtgagcc agtattttta tccgcaaaaa
     8761 cagactcagg tgatgaacga aggctgggcc accttctggc actacaccat ccttaaccat
     8821 ctgtatgatg aagggaaagt aacggaacgt tttatgctgg agtttttgca cagccacacc
     8881 aatgtggtct tccagccccc ctataacagc ccgtggtaca gcggcatcaa cccgtatgcc
     8941 ctcgggttcg ccatgttcca ggatattaaa cggatttgtc agtcgccaac ggaagaagac
     9001 aaatactggt tcccggatat cgccggttcc gactggctgg aaacgctgca tttcgcgatg
     9061 cgtgatttca aagatgagag ttttatcagc cagttcctgt cgccgaaagt gatgcgtgat
     9121 ttccgcttct tcaccgtgct ggatgatgat cggcataatt atctggagat ttccgctatt
     9181 cataatgaag aaggttatcg ggagatccgt aaccggctat cgtcgcaata taacttaagt
     9241 aatctggagc cgaatattca gatctggaac gtggatttgc gcggcgaccg ttcgctgacg
     9301 ctgcgttaca ttccacataa tcgcgcaccg ctggatcggg ggcgcaaaga agtactgaag
     9361 catgtgcatc gcctgtgggg atttgatgtg atgctggaac agcaaaacga agacggcagc
     9421 gtcgagttgc tggaacgttg cccgccgaga atgggaaatc tgtaaaaaca acaaaaaacc
     9481 cctcgtttga ggggtttgtt ttttaaacgg aagggattat cgcccctgaa tggctaaatc
     9541 acccggcaga tttttctgca tccggtgcca aatctcgcca ctctcatgcc catagcgacg
     9601 cactgtttcg tacacctgat cgtgcgcgcc ttcactgcac aacgccgaca gtttgtggta
     9661 gaagcccagc gccagactgc gcgcttccgg attggcgaaa tagtgacgac caatgcgcgt
     9721 atacagtccc ttcatcccgt taaggatcag accgtaaatc gggttgccgg aagcaaacgc
     9781 caggccgcgg aatatgttgt aatccagctc ggcaaaggca tcggcgtgat cggccacttc
     9841 attagcggta gccagcactt cctgcgcttt atcgggatgc tgacgaaacg cggtgcgaat
     9901 aaaaatagtt gaaatattgg tacgcaccga cagcaaatta tcaataagct gcggcacact
     9961 ttcgtgatcc agtcgcgcca gtgtttcaag gatatttaaa ccagaggttt cccagaaatt
    10021 attcaccttc gtcggcttgc catgttgaat ggtcaaccag ccatctcgtg ccagacgctg
    10081 taacacttca cgtaacgtag tacgcgttac gccaattaat tctgaaagtt cacgttctgc
    10141 gggcaaaata gtcccgggag ggaagcggtt attccagata ctttcaataa tgtactcttc
    10201 cgcgaaaccc gccgggcttt gcgccttaat gaccatagtg agatttccat aacacagcaa
    10261 aacaaagttg gactcatcat accagagggc taactacgct gatagcagac aaaaagaaga
    10321 aaaaagggat caaggcttca ttttccgcat ctctttcccc ttgactatca agacttgcct
    10381 cactcctgcc tgaccgttaa gctttgcgtt taaacataaa aacatcattg attatttgta
    10441 aggtaaggga aaccatcatg gagatctcct ggggccgcgc gctatggcgc aattttttgg
    10501 gccagtcccc cgactggtac aaactcgccc tcatcatttt cttaatcgta aatccgttaa
    10561 ttttcctcat cagccctttc gtcgctggct ggttgctggt agcggaattt attttcactc
    10621 tggcgatggc cctgaaatgc tacccgctgc tccccggtgg cctgttggct atcgaagcgg
    10681 tattcatcgg catgaccagc gcggaacacg tccgtgaaga ggtggcggca aatcttgaag
    10741 tcttgctgtt actgatgttt atggtggcgg gtatctattt tatgaaacag ctattgctgt
    10801 tcatatttac ccgtttgctg ttaagcattc gctccaaaat gctgctgtcg ctctcttttt
    10861 gcgtagcggc agcgttcctc tccgcgttcc tcgatgcctt aaccgtcgtg gcggtggtga
    10921 tcagcgttgc cgtcggtttt tatggtattt atcatcgcgt agcctcttcc cgtaccgaag
    10981 acaccgacct gcaagacgat agtcatatcg acaagcatta caaagtggtt ctggaacagt
    11041 tccgtggctt tctgcgtagc ctgatgatgc atgccggtgt cggcaccgca ttaggcggcg
    11101 taatgaccat ggtgggcgaa ccacagaacc tgatcatcgc taaagcggct ggctggcatt
    11161 ttggcgattt cttcctgcgc atgtcgccgg tgaccgttcc ggttctgatt tgtggcctgt
    11221 taacctgcct gctggtagag aagctgcgtt ggtttggcta cggtgaaacg ctgccggaga
    11281 aagtccgcga agtgttgcaa cagtttgacg atcaaagccg ccaccagcgt acccgtcagg
    11341 ataaaatccg tctgattgtc caggcgatta ttggcgtctg gctggtgact gcgctggcgt
    11401 tgcatctggc ggaagttggc ttgattggtt tgtcagtcat tattctggca acatcattga
    11461 ccggtgtcac cgatgaacat gctatcggta aagccttcac cgaatctctg ccattcaccg
    11521 cactgttgac ggtgtttttc tcggtagtcg cggtgattat cgaccaacaa ctgttttcgc
    11581 cgattattca gtttgtgttg caggcatcgg aacatgctca gctgtcgctg ttctatattt
    11641 tcaacggtct gctgtcatcc atttcggata acgtcttcgt ggggacgatt tatatcaacg
    11701 aagcgaaagc ggcaatggaa agtggcgcta tcacgttgaa gcaatacgag ctgctggcgg
    11761 tcgccattaa taccggtacc aatctgccct ctgtcgctac gccgaacggt caggctgcgt
    11821 tcctgttcct gctgacctct gcactcgcgc cattgattcg cctctcttat ggccgcatgg
    11881 tgtggatggc cctgccttac accctcgtcc tgacactcgt cggactgctc tgcgtcgagt
    11941 ttacgcttgc ccctgtaacc gaatggttta tgcaaatggg ctggatagca acgctttgat
    12001 aacaacttac cgggcatata aatgcccggt ttgccttttc gcccgataat tgtccaattg
    12061 cgtcctattt ttgctgcctc ctggtggcgc agcgaatgaa ttggtttaaa ctgcgcactc
    12121 tatgcatatt gcagggaaat gattatgtta cgatttttga accaatgttc acaaggccgg
    12181 ggcgcgtggc tgttgatggc gtttactgct ctggcactgg aactgacggc gctgtggttc
    12241 cagcatgtga tgttactgaa accttgcgtg ctctgtattt atgaacgctg cgcattattc
    12301 ggcgttctgg gtgctgcgct gattggcgcg atcgccccaa aaacgccact gcgttatgta
    12361 gcgatggtta tctggttgta tagtgcgttc cgcggtgtgc agttaactta cgagcacacc
    12421 atgcttcagc tctatccttc gccgtttgcc acctgtgatt ttatggttcg tttcccggaa
    12481 tggctgccgc tggataaatg ggtgccgcaa gtgtttgtcg cctctggcga ttgcgccgag
    12541 cgtcagtggg attttttagg tctggaaatg ccgcagtggc tgctcggtat ttttatcgct
    12601 tacctgattg tcgcggtgct ggtggtgatt tcccagccgt ttaaagcgaa aaaacgtgac
    12661 ctgttcggtc gctaatccat tcggcgctcc tgcgggagcg cttttttcct gccgctgtgt
    12721 ttatttgacc cgcagtaaat cagaacttcg cgttgtataa cgtggtgaaa gcatggctcg
    12781 cttcatctgc cattgttgct ggatcccctg cccggcaaaa tagagtgttc ctctgccctc
    12841 tttagcattc agtgtatcca ttaccgccat caattgctca ctcccggggc gcggagcgtt
    12901 gtcatcgaat aaattgagct gcgcgactcc ctgactgaag aaatccccca gcatcacgcc
    12961 cgctttttgg tagcgatggc ccgcttgcca gatggcatcc agagatcgcg tagcggcgtt
    13021 aatgatatcc ctgctgtcct gagtgggcgt cagcagtttt accgacgcgc tattgccgta
    13081 ataaggttca ttgagcgcaa atggtgacgt cttaataaat gtggagataa accgacaata
    13141 ttgatgctca ctgcgaagtt tttccgccgc ccgggcagcg taactacaaa tggcctgccg
    13201 catcgacgta taatccgtga tgcgttcacc aaacgagcgg gaacagataa tttcctgctt
    13261 tgtcggtgca aattcttcca gttgcaaaca gggttcaccg cgcagttcac gcaccgttct
    13321 ttcgagcacg acattaaaat gtttacggat aaaccggata tctgtatccg ccaaatcgag
    13381 aacggttttg atccccatcg cgtccagttt tttgctgatc cgccgtccaa tcccccagac
    13441 gtcatccacg gggagagcag acattaattt acgctggcgt tccagatttg ataaatccac
    13501 caccccaccc gtctgccgct gccatttttt tgccgcatga ttggcaagct tagccagcgt
    13561 tttggtctgg gcgatcccca caccaacagt aagatgggta cgttgtagca ccgttgcgcg
    13621 aatttctctg ccaaagtcag tcagatcgcg acaattacgc acacctgtca gatcgcagaa
    13681 tgcctcatca atactgtaaa tctcgacgcg gggcgatagt tcttccagcg tcgacatcac
    13741 ccgattgctc atgtctgcgt aaagctcata attgctgcta aagcaaacca cgccacagcg
    13801 acgaaacaga tctttttgtt tgaaccaggg atcgcccatt ttaacgccaa gcgcctttgc
    13861 ctcagcgttt cgggcgataa cgcaaccgtc attattcgat agcacaacca ccggtttacc
    13921 ccataactca gggcgaaaca ccgtctcaca gctggcataa aacgcgttta catcacagag
    13981 ggcaaacatc agcgcatcgc cttaacgacg tggatcacca caccaaagac atccagcgta
    14041 tcttcactac tgatggtaat gggcgagtac gcgctgttca tgggaataag ctgtaccgtc
    14101 gggcgtagtt gcaatttttt caccgtaaac tcgccgtcaa cagcagcgat gacaatatca
    14161 ccatggctgg cggtaatagc gctatcgaca atcagtaaat caccgtcact aattccacca
    14221 tcaatcatag aatcaccact tgctttgacg aagtaagtcg cgctgggatg ctggatcaac
    14281 agttgattca gatcgatgcg ctgttcaacg taatctgcgg ccggtgaagg aaagccacac
    14341 tgaacaagat cgctaaatag cggaaaagtc acaatttcgc ggagatccgc aggcttgata
    14401 aacaacataa taatctgcct gaagttatac tgtttttata tacagtagtc tgttcttgcc
    14461 agcagatcaa tactgattca ggctatcaat atttgtcgct gcatagactg ctgatttttc
    14521 gttctcttat cctgtgctca cgcggccttc tggcgacgac gctcatccag cagaaatgaa
    14581 aaatatcacc cggctaaaaa atagaataga agcattcgcc ataatgacat taaacattgt
    14641 ttgatattta tcatattaat agaaataaag acattgacgc atctctcccg gctaactatg
    14701 aattagatga agtaaaattc attaatagtt gtaaaacagg agtttcatta caatttatat
    14761 atttaaagag gcgaatgatt atgactgaaa tcgttgcaga taaaacggta gaggtagtta
    14821 aaaacgcaat cgaaaccgca gatggagcat tggatcttta taataaatat ctcgatcagg
    14881 tcatcccctg gcagaccttt gatgaaacca taaaagagtt aagtcgcttt aaacaggagt
    14941 attcacaggc agcctccgtt ttagttggca atattaaaac cttactaatg gatagccagg
    15001 ataagtattt tgaagcaacc caaacagtgt atgaatggtg tggtgttgcg acgcaattgc
    15061 tcgcagcgta tattttgcta tttgatgagt ataatgagaa gaaagcatcc gcccagaaag
    15121 acattctcat taaggtactg gatgacggca tcacgaagct gaatgaagcg caaaaatccc
    15181 tgctggtaag ctcacaaagt ttcaacaacg cttccgggaa actgctggcg ttagatagcc
    15241 agttaaccaa tgatttttca gaaaaaagca gctatttcca gtcacaggta gataaaatca
    15301 ggaaggaagc gtatgccggt gccgcagccg gtgtcgtcgc cggtccattt ggtttaatca
    15361 tttcctattc tattgctgcg ggcgtagttg aagggaaact gattccagaa ttgaagaaca
    15421 agttaaaatc tgtgcagagt ttctttacca ccctgtctaa cacggttaaa caagcgaata
    15481 aagatatcga tgccgccaaa ttgaaattaa ccaccgaaat agccgccatc ggggagataa
    15541 aaacggaaac tgaaacaacc agattctatg ttgattatga tgatttaatg ctttctttgc
    15601 taaaagaagc ggccaataaa atgattaaca cctgtaatga gtatcagaaa agacacggta
    15661 agaagacact ctttgaggta cctgaagtct gatcagcaat tattctctcc atgtactcaa
    15721 ggtataaagt ttatcacatt taaaaataca gacgtttttt aaagattgag agttacctgt
    15781 tgataataag gtatcgggta actctcatta tgacagagcg ctttcaaaaa cagggctacg
    15841 aaatggcgag catccagacg tatattaaga aagcccccat caacatctat taaatttatc
    15901 actgcgccca gtcaggttta tttaagatat gatcctgcca gtcaatcact tctgattctt
    15961 tcactgcgat atgacgcaca gagatacgtt caccatgcat tgccgctttc gaaccagtaa
    16021 gcagcggatg ccacgcaggt aaatctttac cttccgccag caaacgataa gcgcaggtca
    16081 ttggtagcca ttcgaatgtt ggcagatttt cacgggttaa tttaatgcag tcgggttcaa
    16141 actcgaaacg acgttcgtag ttccgacatt gacaggtttt aatattgagc tggcgacagg
    16201 cgacgttagt gaagtagatt tcgtcggtgt cttcatccat cagtttatgc aggcaacact
    16261 gaccgcaacc atcacacaac gactcccatt ccgcatcgct catttcgtcc agggttttac
    16321 tttgccagaa aggtacatcg ctcatcaggt gttccgccat tacgttaaaa cgcaccttat
    16381 aaccagtctg gcacagcgat gcaagttttg ccgccgcttt caggcggcaa aaagtattac
    16441 aaaacgcgag ttgtcaaaga atgcccatcg aaagtgactg tcagctcatc accgctttgc
    16501 aacgggccga cgccatcagg cgtgcctgtc agcacaacgt caccggcctt gagggtaaaa
    16561 aacttgctca tataagcgat cagcggaacg attttatgga tcatgtccgc agtcgtacct
    16621 tgctggcgtt gttcgccgtt tacgctcagg cccagcgttg tattttgcgg atcgccggtg
    16681 aattccgccg cgggaataaa cccggaaagc ggacaagagt tatcaaacgc tttagccttt
    16741 tcccacggct gcccggcttt cttcattttt ccctgaacat cacgcaacgt cagatcgagc
    16801 gccacgccgt aaccggcaat ggctttgcgg acatgctctt ccgtagcctg acgcagcgtc
    16861 gcgccaatca acaccgctag ttcgacttca tgatgaactg aaccgaaatc ggatgggatc
    16921 gccaatggct gccgcagatc gcacagtgcc gtttctggtt taataaacag cactggctct
    16981 tcgggcactg cgctgcccat ctctttaata tgtttggcat agttactgcc aacacaaact
    17041 actttactca ccggataatc cagcagcgca ccttgccagt tgtgatgttg atacataacg
    17101 ttcccctcac cttatcgtgg atatgaaaac ccgcgccgac aaagataatg ccaccgccgg
    17161 atatcggtta tttgttagtg tcgtctgttt tctgccccat gacggaaaga tgttgcttca
    17221 gcaaatcttc gggtggtggc ggtaactgca aatagtaacc ttgctcggtt aatgcctgtt
    17281 taaccttttc aatatcggca ttgaccagtt tcttccgccc atccagcggc agaatcatcg
    17341 ctaactgagg ctgaccaaaa cctttcatca gttcctcagg aacacgcgaa aaatcgtctt
    17401 ttttttcgac atataaatag gtctggtcac gcttgctgct tcgataaatc acacaaaaca
    17461 tacttttact ctttaagatg ccaggttttg gcataagcta acatcaatga aacccgcgcc
    17521 acaagccagc cagctgtaaa tttctacggt ttgttgagtc catgcccctt tataagtaat
    17581 ccccccggca aaaatgccgc gacgggattt taccgaacgc aggaaagcca ctggaattct
    17641 cccgtaaggg attgttatat tataacagtt catcgtactc attctgaaca gaagactacc
    17701 aatgaaaatc aagagcatca gtaaggctgt attgctgctt gcactgctaa cctccacgag
    17761 ctttgctgcg ggtaagaatg tcaatgttga gttcagaaaa ggccatagca gcgctcagta
    17821 ctccggcgaa ataaagggat acgattacga tacatatacc ttctatgcca aaaaaggtca
    17881 gaaagtgcat gtgagtatct ctaatgaagg cgctgatact tacctcttcg ggccaggaat
    17941 cgatgattct gttgatttat ccagatattc ccctgaactc gatagtcacg gacaatactc
    18001 actccctgct tctggcaaat atgagctgcg agtgctgcaa acccgaaacg acgcccgtaa
    18061 aaacaaaact aaaaaatata acgtcgatat tcaaattaag taaatgtcaa ccgacgggga
    18121 gtctccccgt cataaacgtt gacgccccac tcctctctgt cgctcaagag gttaatgacg
    18181 aatagaacat aatgtaaaca agacatcact cacgacacat aaataacatc tttgtggtgg
    18241 tttgattgat ttttccactc cgcttaccat ctacaccaaa ataacgatca tcacggcata
    18301 atttttcttt aacatcgcaa aatacaccgt tagcgaatgt aaattcgctg ggatcgtagc
    18361 cttgtaatga ttgtaagttt tcagcggctt tttcgctgag atatttcctt gttaactctg
    18421 gggaaatgcc atctgagttc acacaaatat acgggtcgca aacaacgccg cacgccggag
    18481 attgaagtcc gccagcgtca tgtccagacg ccattgatga gaacatcatc cccacacctg
    18541 acaatccgat ataaaaaata cgcatcatat tcatagcaca tcctgataat ccaaccgtac
    18601 aacacttatg ctcatttcat catcaatccg taatcaaaac cgctgacctg gacgagttac
    18661 gatgacctga cagaagtatc agacgtcttc tgcggtttct atgatagagc aattctgtgg
    18721 cggatattaa gataacgggt atctgttctt taatgatgct gcaaaaaatg gaaagttaag
    18781 aaagcctggc gtggtgaaaa aatacacttg cctcaatact ggatatttaa acaggtctgg
    18841 agatgctgtc acgattaggg tgcggaaaaa ggaagcgtaa ataagaagaa tcaaaagtac
    18901 gacggcaatg ggttgattga caagggtatt ttttaagcta tgaatcagcg ccatttatca
    18961 cagaatagac ttttactctg aataaatggg agggtgactt gcctcaatat aatccagact
    19021 ataacatgcc ttatagtctt cggaacatca tcgcgcgctg gcgatgatta atagctaatt
    19081 gagtaaggcc aggatgtcaa acacgccaat cgagcttaaa ggcagtagct tcactttatc
    19141 tgtggttcat ctgcatgagg cagaacctaa ggttatccat caggcgctgg aagacaaaat
    19201 cgctcaggcc cccgcatttt taaaacatgc ccccgttgta ctcaacgtca gtgcactgga
    19261 agacccggta aactggtcag cgatgcataa ggcggtttcg gcaaccggtt tgcgggttat
    19321 tggcgtaagt ggctgcaaag atgcgcaact taaagccgaa attgaaaaaa tggggctgcc
    19381 tatcctgacg gaaggaaagg aaaaagcgcc acgtccagct cccgcaccgc aggctccagc
    19441 gcaaaataca acgccggtca caaaaacgcg tttaatagat accccggtgc gttccggtca
    19501 gcgtatttat gctccacaat gtgatctgat tgttacaagc cacgttagcg ctggggccga
    19561 attgattgcc gatgggaaca ttcatgtcta tggcatgatg cgcggtcgtg cgctggcagg
    19621 ggcaagtggt gaccgggaaa cgcaaatatt ttgtacgaac ctgatggcgg aattggtgtc
    19681 catcgcaggt gaatactggc tgagtgatca aatcccagca gaattttatg gcaaagcggc
    19741 gcgactgcag ttagtcgaaa acgctttgac cgttcaaccg ttaaattgat ccctttttaa
    19801 caaggaattt ctatggcacg cattattgtt gttacttcgg gcaaaggggg tgttggtaag
    19861 acaacctcca gcgcggccat cgccactggt ttggcccaga agggaaagaa aactgtcgtg
    19921 atagattttg atatcggcct gcgtaatctc gacctgatta tgggttgtga acgccgggtc
    19981 gtttacgatt tcgtcaacgt cattcagggc gatgcaacgc taaatcaggc gttaattaaa
    20041 gataagcgta ctgaaaatct ctatattctg ccggcatcgc aaacacgcga taaagatgcc
    20101 ctcacccgtg aaggggtcgc taaagttctt gatgatctga aagcgatgga ttttgaattt
    20161 atcgtttgtg actccccggc agggattgaa accggtgcgt taatggcact ctattttgca
    20221 gacgaagcca ttattaccac caacccggaa gtctcctcag tacgcgactc tgaccgtatt
    20281 ttaggcattc tggcgtcaaa atcacgccgc gcagaaaatg gcgaagagcc tattaaagag
    20341 cacctgctgt taacgcgcta taacccaggc cgcgtaagca gaggtgacat gctgagcatg
    20401 gaagatgtgc tggagatcct gcgcatcaaa ctcgtcggcg tgatcccaga ggatcaatca
    20461 gtattgcgcg cctctaacca gggtgaaccg gtcattctcg acattaacgc cgatgcgggt
    20521 aaagcctacg cagataccgt agaacgtctg ttgggagaag aacgtccttt ccgcttcatt
    20581 gaagaagaga agaaaggctt cctcaaacgc ttgttcggag gataagttat ggcattactc
    20641 gatttctttc tctcgcggaa gaaaaataca gccaacattg caaaagaacg gctgcagatt
    20701 attgttgctg aacgccgtcg cagcgatgca gaaccgcatt atctgccgca gttgcgtaaa
    20761 gatattcttg aggtcatttg taaatacgta caaattgatc ctgagatggt aaccgtacag
    20821 cttgagcaaa aagatggcga tatttctatt cttgagctga acgtgacctt accggaagca
    20881 gaagagctga aataatcccg ctgtaaaagc gcatttatct tcaaggcaga gttatctctg
    20941 ccttgagttt ttcatccctc tcatccacgt tgtggtaaag cggcgagtat tcttgctgat
    21001 actcctcatt gctatttcca cctccccctt tacaccttaa ggctgttccc acgcacaaaa
    21061 caaaatattt atgcctggtt gagtaaataa ccttattgtt agtgttgata tactgggaga
    21121 tgataacccc gcaatttcat tattcattgt tctcccatag gatgaggttt cccgccgtaa
    21181 ctggcgggct ttttttaccc cagaattttc tcttttaaca tcctgtaaac gaaaactgcg
    21241 ccgaagcgca gtttaatcgt tgatggtcag aacgaccagt tcacaccagc caccgcgttc
    21301 cacggggatt ccataccagc accgttgcta taccccaccc caagatgccc gcttaacgta
    21361 ctgctgaatg aggctttaat acctgcctgg tatattccac gtctgcccga caaatcattg
    21421 acgaaattac cgtcactatt cactttcacc cggttatcat cgacaaattc tttgcgcaca
    21481 gccgccttca gccacggctc aacttccata ccgttcccca gacgcatgtt gtaactcagc
    21541 gttgcaccca gttcacgata tatactgcgg gtatcgactg atttcgattc catgccattg
    21601 gataaatgat attcggggtt atcagcggtg aaccccgtta acgaggcata cggcgtcagg
    21661 ttccagttac catcggtaaa tcgcatcccg gtttcaatgt gaccgcccag cccgttgcta
    21721 tggtaactgc cattggcggc tccaccgctg ctcattttac cggctacgtt actttcaaaa
    21781 cggttcagct tcacgacacc gtccagatag aaaccacttt catgttccca actggcatag
    21841 ccgcccagag aataactgtc cacactgcca tgtcctccac gatcaaaacc gatatgtgaa
    21901 tgggaataac ccataaaagc gcccagcgtc gcaatcccct caggaatatc attacggctg
    21961 tcgatcccca ctgtcattcc ggtcagcgtc tgctcaaacc cggcacccgc atcatttatc
    22021 aacatatggg ctttaaactt tcaggacgtt ccgagcggga caatcaaggc agaccctacc
    22081 ctctattaca tctttataac gcaatgtgac atgataattt taggagaatt acatcatgga
    22141 tttttttggc tgaaagcgac aatgtaagaa caactctggc cgctatctgg gtttctcatt
    22201 tggtactcgc tggcaaaggt aagagcaaga caatcaccgg cttgtaaatg atgagtctga
    22261 tgattgactc gaaaatcaag gctgccgctt aacatccata gagtttgtcc agaaagatgc
    22321 tcatttgcag aggcagggat agtcaattgc cccatagggg ggatttcaac ttttattagt
    22381 tccggacatg cacccgctgg ggacaaagac cagcgggtaa tgcctgtttc ttcatcaatc
    22441 caatgttgtt gctggtcagc caaaagtaca agtgaattct gttgcatctc aagctctgca
    22501 aaaagctttg atagcgtaat attcagtgca ttagctaacc gactaagtat tgttgcactg
    22561 gggcttgaag tgcctcgttc aactttgctg atcatcgcct gactgacacc agatcgttgg
    22621 gcaagctctg taacagtcaa gtttcgagac tttctgagct tgagtaacaa acgtgcaata
    22681 tcgctatcga ctatctgatt tttagctctt ttatccattc ttctcccctc tttcaccgac
    22741 ctgattatgc cattacataa attgcgatta aataaatgat tttacacgtc tcaaatgcat
    22801 tttttctata cgttatagct ttagagtaag agctggtaac ttcaacttcc ttatttggca
    22861 cataacagtc ctggatacag tgacgcacag tttctttaca aaaacctagc tacatagagg
    22921 ttaatcatcg tgtcaaatgt actgtagaaa tac
//
LOCUS       DABSYT020000071        22504 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.071, whole genome shotgun sequence.
ACCESSION   DABSYT020000071 DABSYT020000000
VERSION     DABSYT020000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.071"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            407..687
                     /gene="drpB"
                     /locus_tag="ID725_RS16300"
                     /old_locus_tag="pgaptmp_003260"
                     /pseudo
     CDS             407..687
                     /gene="drpB"
                     /locus_tag="ID725_RS16300"
                     /old_locus_tag="pgaptmp_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
     gene            727..1422
                     /gene="yedJ"
                     /locus_tag="ID725_RS16305"
                     /old_locus_tag="pgaptmp_003261"
     CDS             727..1422
                     /gene="yedJ"
                     /locus_tag="ID725_RS16305"
                     /old_locus_tag="pgaptmp_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HGV7851793.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1489..2907
                     /gene="dcm"
                     /locus_tag="ID725_RS16310"
                     /old_locus_tag="pgaptmp_003262"
     CDS             1489..2907
                     /gene="dcm"
                     /locus_tag="ID725_RS16310"
                     /old_locus_tag="pgaptmp_003262"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGV7851794.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2888..3358
                     /gene="vsr"
                     /locus_tag="ID725_RS16315"
                     /old_locus_tag="pgaptmp_003263"
     CDS             2888..3358
                     /gene="vsr"
                     /locus_tag="ID725_RS16315"
                     /old_locus_tag="pgaptmp_003263"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGV7851795.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3347..4267)
                     /gene="yedA"
                     /locus_tag="ID725_RS16320"
                     /old_locus_tag="pgaptmp_003264"
     CDS             complement(3347..4267)
                     /gene="yedA"
                     /locus_tag="ID725_RS16320"
                     /old_locus_tag="pgaptmp_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGV7851796.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4440..5357
                     /gene="yedI"
                     /locus_tag="ID725_RS16325"
                     /old_locus_tag="pgaptmp_003265"
     CDS             4440..5357
                     /gene="yedI"
                     /locus_tag="ID725_RS16325"
                     /old_locus_tag="pgaptmp_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGV7851797.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            5436..5618
                     /gene="yodC"
                     /locus_tag="ID725_RS16330"
                     /old_locus_tag="pgaptmp_003266"
     CDS             5436..5618
                     /gene="yodC"
                     /locus_tag="ID725_RS16330"
                     /old_locus_tag="pgaptmp_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGV7851798.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5789..7483
                     /gene="dgcQ"
                     /locus_tag="ID725_RS16335"
                     /old_locus_tag="pgaptmp_003267"
     CDS             5789..7483
                     /gene="dgcQ"
                     /locus_tag="ID725_RS16335"
                     /old_locus_tag="pgaptmp_003267"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGV7851799.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7480..8295)
                     /gene="yedP"
                     /locus_tag="ID725_RS16340"
                     /old_locus_tag="pgaptmp_003268"
     CDS             complement(7480..8295)
                     /gene="yedP"
                     /locus_tag="ID725_RS16340"
                     /old_locus_tag="pgaptmp_003268"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGV7851800.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8593..8820)
                     /gene="yodD"
                     /locus_tag="ID725_RS16345"
                     /old_locus_tag="pgaptmp_003269"
     CDS             complement(8593..8820)
                     /gene="yodD"
                     /locus_tag="ID725_RS16345"
                     /old_locus_tag="pgaptmp_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGV7851801.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8983..9171
                     /gene="dsrB"
                     /locus_tag="ID725_RS16350"
                     /old_locus_tag="pgaptmp_003270"
     CDS             8983..9171
                     /gene="dsrB"
                     /locus_tag="ID725_RS16350"
                     /old_locus_tag="pgaptmp_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGV7851802.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9215..9838)
                     /gene="rcsA"
                     /locus_tag="ID725_RS16355"
                     /old_locus_tag="pgaptmp_003271"
     CDS             complement(9215..9838)
                     /gene="rcsA"
                     /locus_tag="ID725_RS16355"
                     /old_locus_tag="pgaptmp_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGV7851803.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10128..10913)
                     /gene="fliR"
                     /locus_tag="ID725_RS16360"
                     /old_locus_tag="pgaptmp_003272"
     CDS             complement(10128..10913)
                     /gene="fliR"
                     /locus_tag="ID725_RS16360"
                     /old_locus_tag="pgaptmp_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGV7851804.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            complement(10921..11190)
                     /gene="fliQ"
                     /locus_tag="ID725_RS16365"
                     /old_locus_tag="pgaptmp_003273"
     CDS             complement(10921..11190)
                     /gene="fliQ"
                     /locus_tag="ID725_RS16365"
                     /old_locus_tag="pgaptmp_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGV7851805.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11200..11937)
                     /gene="fliP"
                     /locus_tag="ID725_RS16370"
                     /old_locus_tag="pgaptmp_003274"
     CDS             complement(11200..11937)
                     /gene="fliP"
                     /locus_tag="ID725_RS16370"
                     /old_locus_tag="pgaptmp_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGV7851806.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(11937..12302)
                     /gene="fliO"
                     /locus_tag="ID725_RS16375"
                     /old_locus_tag="pgaptmp_003275"
     CDS             complement(11937..12302)
                     /gene="fliO"
                     /locus_tag="ID725_RS16375"
                     /old_locus_tag="pgaptmp_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGV7851807.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12305..12718)
                     /gene="fliN"
                     /locus_tag="ID725_RS16380"
                     /old_locus_tag="pgaptmp_003276"
     CDS             complement(12305..12718)
                     /gene="fliN"
                     /locus_tag="ID725_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGV7851808.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12715..13719)
                     /gene="fliM"
                     /locus_tag="ID725_RS16385"
                     /old_locus_tag="pgaptmp_003277"
     CDS             complement(12715..13719)
                     /gene="fliM"
                     /locus_tag="ID725_RS16385"
                     /old_locus_tag="pgaptmp_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGV7851809.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13724..14188)
                     /gene="fliL"
                     /locus_tag="ID725_RS16390"
                     /old_locus_tag="pgaptmp_003278"
     CDS             complement(13724..14188)
                     /gene="fliL"
                     /locus_tag="ID725_RS16390"
                     /old_locus_tag="pgaptmp_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGV7851810.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14293..15420)
                     /gene="fliK"
                     /locus_tag="ID725_RS16395"
                     /old_locus_tag="pgaptmp_003279"
     CDS             complement(14293..15420)
                     /gene="fliK"
                     /locus_tag="ID725_RS16395"
                     /old_locus_tag="pgaptmp_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGV7851811.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15417..15860)
                     /gene="fliJ"
                     /locus_tag="ID725_RS16400"
                     /old_locus_tag="pgaptmp_003280"
     CDS             complement(15417..15860)
                     /gene="fliJ"
                     /locus_tag="ID725_RS16400"
                     /old_locus_tag="pgaptmp_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGV7851812.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(15879..17252)
                     /gene="fliI"
                     /locus_tag="ID725_RS16405"
                     /old_locus_tag="pgaptmp_003281"
     CDS             complement(15879..17252)
                     /gene="fliI"
                     /locus_tag="ID725_RS16405"
                     /old_locus_tag="pgaptmp_003281"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGV7851813.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(17314..18000)
                     /gene="fliH"
                     /locus_tag="ID725_RS16410"
                     /old_locus_tag="pgaptmp_003282"
     CDS             complement(17314..18000)
                     /gene="fliH"
                     /locus_tag="ID725_RS16410"
                     /old_locus_tag="pgaptmp_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGV7851814.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(17993..18988)
                     /gene="fliG"
                     /locus_tag="ID725_RS16415"
                     /old_locus_tag="pgaptmp_003283"
     CDS             complement(17993..18988)
                     /gene="fliG"
                     /locus_tag="ID725_RS16415"
                     /old_locus_tag="pgaptmp_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGV7851815.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(18981..20639)
                     /gene="fliF"
                     /locus_tag="ID725_RS16420"
                     /old_locus_tag="pgaptmp_003284"
     CDS             complement(18981..20639)
                     /gene="fliF"
                     /locus_tag="ID725_RS16420"
                     /old_locus_tag="pgaptmp_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGV7851816.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSWRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            20854..21168
                     /gene="fliE"
                     /locus_tag="ID725_RS16425"
                     /old_locus_tag="pgaptmp_003285"
     CDS             20854..21168
                     /gene="fliE"
                     /locus_tag="ID725_RS16425"
                     /old_locus_tag="pgaptmp_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGV7851817.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            complement(21193..21333)
                     /locus_tag="ID725_RS16430"
                     /old_locus_tag="pgaptmp_003286"
                     /pseudo
     CDS             complement(21193..21333)
                     /locus_tag="ID725_RS16430"
                     /old_locus_tag="pgaptmp_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            21517..>22504
                     /gene="espR3"
                     /locus_tag="ID725_RS16435"
                     /old_locus_tag="pgaptmp_003287"
     CDS             21517..>22504
                     /gene="espR3"
                     /locus_tag="ID725_RS16435"
                     /old_locus_tag="pgaptmp_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HGV7851818.1"
                     /translation="MFPLNDLSLKTQSVQLNKITSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNDKFTPVSEDSNLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKTGSITI
                     EKSQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGASNDLKE
                     NVDRALVRMFKCVKLHSNELNLSSLSLGSVPPLPEWIEMLSLVYNELDSIQVPESCKE
                     LELDFNNLTEFPQVPDGITLISVNNNLISYIDSFPPKAKKIFICHNKLSEIPALPDTA
                     KVFDCSENNIKEIRWFPKNLKEAYIEYNKIEVIPAIPGNLKLLCMKCNPIKEA"
BASE COUNT         5441 a         5672 c         5896 g         5495 t
ORIGIN      
        1 gatggggggt atcaatgatt gttgcgctaa acggctggtg tgatcacgcg cacattaatg
       61 aaggcataaa aaaaagccgg catcatgccg gcccaaaaaa tataaacaag acgataaaag
      121 gctaataacg gaagcatcat gacacagaat aataaaaagg tataattgta atcagtgcta
      181 atatattgat tgaatgaatt aataaagccg atctcaaaga taaataaaat cgctcaaaac
      241 gatttttcct gtattcactg ccgttgcgca aaaattatct atttgttcaa aaaatgagtg
      301 agtcttgact ggctcatcca atgtggaaaa atgtgacttt tatcacgtaa tagtactaag
      361 tctgaatttt ccgggttatc tcaaaatgga atacggttcg acaaagatgg aagagagact
      421 ctctcgcagc cctggcggca aactggcttt gtgggctttt tatacatggt gtggctattt
      481 cgtctgggca atggcgcgtt acatctgggt gatgagccgg atccccgatg cccccgtgtc
      541 gggatttgaa agcgatttag gctctaccgc gggaaagtgg ttagggcact ggtcggattt
      601 ttatttatgg ctctggtcgg ggcattgtta ggcagcattg cctggtacac acgacctcgt
      661 cccgcacgta gcagacgcta tgaataaaaa ctgctaaggt ggcgactctt tggcaacgga
      721 gcgcgtatgg acttacaaca ctggcaggca cagttcgaaa actggttgaa aaaccatcat
      781 cagcaccagg acgcggccca tgacgtgtgt cattttcgcc gcgtctgggc tacggcacag
      841 aagctggcgg cagatgatga cgtcgatatg ctggtgattt taaccgcctg ttatttccac
      901 gatattgtga gcctggcgaa aaatcatccg caacggcaga gttcttcaat cctggcggca
      961 gaagagacgc gtcgcctgct ccgtgaagag ttcgtgcaat ttccggcgga gaaaatcgag
     1021 gccgtttgtc atgccattgc cgctcacagt ttcagcgcgc aaatcgcccc cttaacaacg
     1081 gaagctaaaa tcgtccagga tgcggatcgg ctggaagccc tgggggcgat tggcctggcg
     1141 cgcgtgtttg ccgtttcagg ggcattgggc gtggcgctgt ttgatgccga agatccgctc
     1201 gcacagcatc gcccgcttga tgataaacgc tacgcgctgg atcatttcca gactaagttg
     1261 ttgaagctac cgcaaatcat gcaaaccgta agaggcaagc agctggcgca gcacaacgcg
     1321 cattttttag tcgagtttat ggcgaagctc agtgccgaac tggcggggga gaatgaaggt
     1381 gtcgatcaca aggtgataga tgcgttttca cccgcgggct gagcgcgtgg ccctaaatgg
     1441 ctgtaattat gttaacctgt cgaccatctc agatggccgg ttaaatctat gcaggaaaat
     1501 atatcagtaa ccgattcata cagcaccggg aatgccgcac aggcaatgct ggagaaactg
     1561 ctgcaaattt atgatgttaa aacgctggtg gctcagctta atggtgtggg tgagaatcac
     1621 tggagcgcgg caattttaaa acgtgcgctg gcgaatgact cggcatggca ccgtttaagt
     1681 gagaaagagt tcgcccatct gcaaacgttg ttacccaaac caccggcaca tcatccgcat
     1741 tatgcgtttc gctttatcga tctatttgcc ggaattggcg gcatccgtcg cggttttgaa
     1801 tcgattggcg gacagtgcgt gtttaccagc gaatggaaca aacatgcggt acgcacttat
     1861 aaagccaatc attattgcga cccggcgacg catcatttta atgaagatat ccgcgacatc
     1921 accctcagcc ataaagaagg cgtgagtgat gaggctgcgg cggaacatat tcgtcaacac
     1981 attcctgaac acgatgtttt actggccggt ttcccttgtc agccattttc gctggctggc
     2041 gtatcgaaaa agaactcgct cgggcgggcg cacggttttg cctgcgatac tcagggcacg
     2101 ctgttctttg atgtggtgcg cattatcgac gcgcgtcgtc cggcgatgtt tgtgctcgaa
     2161 aacgtcaaaa acctgaaaag ccacgaccag ggtaaaacgt tccgcatcat catgcagacg
     2221 ctggacgaac tgggctatga cgtggctgat gcagaagata acgggccgga cgatccgaaa
     2281 atcatcgatg gcaaacattt tctgccgcag caccgtgaac gcatcgtgct ggtgggtttt
     2341 cgtcgcgatc ttaatctgaa agccgatttt actctgcgtg atatcagcga atgtttccct
     2401 gcacagcgag tgacgctggc gcagctgttg gacccgatgg tcgaggcgaa atatatcctg
     2461 acgccggtgc tgtggaagta cctctatcga tatgcgaaaa aacatcaggc gcgcggaaac
     2521 ggcttcggtt atggaatggt ttatccgaac aatccgcaaa gcgtcacccg tacgctgtct
     2581 gcgcgttatt acaaagatgg cgcggaaatt ttaatcgatc gcggctggga tatggccacg
     2641 ggtgagaaag actttgacga tccgctgaat cagcaacatc gtccacgtcg gttaacgcct
     2701 cgggaatgcg cgcgcttaat gggttttgaa gcgccgggag aagcgaaatt ccgcattccg
     2761 gtttcggaca ctcaggccta tcgccagttc ggtaactcgg tggtcgtgcc ggtctttgcc
     2821 gcggtggcaa aactgcttga gccaaaaatc aaacaggcgg tggcgttgcg tcagcaagag
     2881 gcacaacatg gccgacgttc acgataaggc cactcgcagc aaaaatatgc gcgcgattgc
     2941 cacgcgtgat acggcgattg agaagcgcct cgccagtctg ttaaccgggc agggcctggc
     3001 gtttcgcgtt caggacgcca gtctgcccgg acgcccggat tttgtcgttg atgaatatcg
     3061 ctgcgtgata tttacccatg gctgcttctg gcatcatcat cactgctatc tgtttaaagt
     3121 gcctgcgact cgaaccgagt tctggctgga gaagataggt aaaaatgttg agcgcgatcg
     3181 ccgcgatatc agtcgcttgc aggaactcgg ctggcgcgta ttgattgtct gggagtgcgc
     3241 gttacgtggg cgcgagaagc tgaaggatga agcgcttacc gagcgtctgg aagagtggat
     3301 ctgcggcgaa ggagccagcg cgcagatcga cacgcagggg atacatttac tcgcttgatg
     3361 cgtcctgaat aactggagca actacgggtt ttgccggaaa gagatatttt cccaacgtga
     3421 ccagtaccac cgcgaagaca attacgccga gcgccagcca ttcaatcttc gacagtgttt
     3481 ctccacccag tcccgtaccc agcaagaccg cgaccaccgg gttaacgtaa gcgtagctgg
     3541 tggcgagagc cggactgaca ttacggatta aatacatata agcgttgatg gcgataatcg
     3601 aaccaaacag cgccagatag ccgaccgcaa ggaagcctga aagggaaggg agcgccgtca
     3661 gtttttcacc cgcaatcatc gacgcgatca ttaacaccac gcctgccgcc agcatctcaa
     3721 tcgcacccgc catcatccct acaggtaagg taatgcgcga gccataaact gagccaaacg
     3781 cccagctaat cgagccgatt aaaatcagaa tcgcgcccca cggattgccg cttaaatttc
     3841 caccgctatt gagcatgatg attccggcaa gcccaatggc aatacccacc cattccagtt
     3901 tgcgcgtttt aatgccaaac aggcggctga agcacagggt aaagaggggc acggttgcaa
     3961 ccactacggc ggcgatgccg gaaggaacat tttgatgttc ggcaaccgtc accatgccat
     4021 taccgacagc cagcaataac aggccaatca gcgcggcatt gagcagcgga cgtagcgggg
     4081 ggagtttgtg tccgcgcagt agtaaaaatg ccagcaataa aataccggct gccaggaatc
     4141 gaacacccgc catcattaac ggaggccagc tttccacgcc aatccgaatg acaaaatagg
     4201 ttgagcccca aatgatatac aacgcaaaca gcgcgccaaa aagcggtaac aactggcgga
     4261 aacgcataat ccctcacggt ggaaataaaa aggtggttca tagtaaacgt gaaaatcatt
     4321 ctgctggcga gagatataat tgcacttgat tgttaaaaaa atgttgacct gtgaagcaag
     4381 tatcagagct gccgtttttg cttcatactt acacccttca acaataaaaa tgagagggaa
     4441 tgcttttggc cgggagtagt ttactgacgt tgctcgatga tatcgccaca ctgctggacg
     4501 atatctccgt gatgggcaaa ctggcggcga agaaaaccgc cggtgtatta ggggatgact
     4561 tatcgctcaa tgcgcaacaa gtttcaggcg tgcgggccaa ccgggaactt cccgtggtct
     4621 ggggcgtggc gaaaggatcg ctgattaata aagtgattct ggtgccactg gcgctgatca
     4681 tcagtgcgtt tatcccgtgg gcgattacgc cattgttgat gatcggtggc gcgtttctct
     4741 gctttgaagg ggtagagaaa gtgctgcata tgctggaggc gcgtaaacat aaagaagatc
     4801 cggcgcagag ccagcagcgt ctggagaagc tggcggcgca ggatccgctg aagtttgaaa
     4861 aggacaaaat aaaaggggcg attcgtaccg attttatatt gtctgcggaa atcgtcgcca
     4921 tcacgctggg gattgtggcc gaagcgccgt tgcttaatca ggtgctggtg ctttcaggca
     4981 tcgcgctggt agtgaccgtg ggcgtctacg gtctggtagg ggttatcgtt aagattgatg
     5041 acctggggta ttggctggcg gaaaaatcca gcgcgctgat gcaggcatta ggtaaaggat
     5101 tattgattat cgcgccctgg ctgatgaaag cgttatcgat tgtcggcacg ctggcgatgt
     5161 tcctcgtcgg cggcgggatt gtggtacatg gtattgcgcc gctgcatcac gccattgaac
     5221 atttcgccgg gcagcaaagt gcagtggtgg cgatgatatt accgactgtt ttaaatctga
     5281 ttcttggatt tatcatcggc ggcatcgtgg tgctgggagt gaaagccgta gcgaaaatgc
     5341 gcggtcaggt acattaatac tcgcacaggc tattatgcaa aattcgccat cttcaactaa
     5401 ggtgaacaag tttcactcga aaaaaggagg cgagtatgag ctttatggtt agtgaggaag
     5461 ttacggttaa agagggcggc ccgcggatga ttgtcactgg atactccagc ggtatggttg
     5521 agtgtcgttg gtatgacggt tacggggtca agcgggaagc ttttcatgaa accgagcttg
     5581 ttccagggga ggggagtcgt tctgcggaag aagtttgaaa gggtatcatt aagaccaggc
     5641 cggatcacat ccggcctggt gtgccaatgc ctgcaatgaa tgatagtcgc gtcttatcat
     5701 gccaccatta taaaattatc gctgttttgc ggtacgctat cgccaggtta tgccagaatc
     5761 ataaaaaagc aggtggggag tcgtcagggt gcagcacgag acaaaaatgg aaaaccagag
     5821 ctggttgaaa aaactcgcac gccgcctagg gcctggtcat atcgttaatc tctgctttat
     5881 cgtggtattg cttttttcca ccttgctgac ctggcgtgaa gtagtggtgc tggaagatgc
     5941 ctatatctcc agccagcgta atcatctgga aaacgtagcc aacgcgctcg ataagcattt
     6001 gcagtataac gtcgacaaac tgatcttttt gcgtaatggc atgcgcgaag ctctcgtagc
     6061 gccactggat ttcacctctc tgcgtgatgc tgtaaccgag ttcgaacagc atcgcgacga
     6121 gcacgcctgg caaatcgaac tcaaccgacg acgcaccctg tcagtcaatg gcgtatcgga
     6181 tgcattagtc agcgagggga atctcctgtc tcgcgaaaat gaaagcctcg acaatgaaat
     6241 taccgctgca ctggaagttg gttacttgct gcgactggca cacaactcct cgtcgatggt
     6301 agaacaggcg atgtatgtct cgcgtgccgg attttacgtt tcgacgcagc cgaccttgtt
     6361 tacgcgcaat gtcccgacgc gttattacgg ctatgtcacc caaccctggt ttatcggcca
     6421 ttcacaacga gaaaatcgtc accgcgcggt acgctggttc acttcgcaac cggaacacgc
     6481 cagcaatact gaaccgcagg ttaccgtcag tgttccggta gacagtaata actactggta
     6541 tggcgtgctg gggatgagta ttcccgtgcg tactatgcag caatttttaa gaaacgccat
     6601 cgataaaaac ctcgatggtg agtatcagct ctatgacagt aagctgagat ttttgacctc
     6661 ttccaatcct gaccatccaa cagggaatat ttttgatcct cgtgaactgg ccttgctggc
     6721 gcaggcaatg gaacatgaca cgcggggcgg cattcgtatg gacagtcgct atgttagctg
     6781 ggaacgtctg gaccatttcg acggtgtgct ggtgcgcgtc catacgttaa gcgaaggcgt
     6841 gcgcggcgat ttcggcagta tcagcattgc attaaccctg ctgtgggcgc tctttaccac
     6901 catgttactc atctcctggt atgtgattcg ccggatggtc agcaacatgt atgttctgca
     6961 aagctcgttg cagtggcagg cgtggcacga caccttaacg cgtttatata accgtggcgc
     7021 actgttcgaa aaagcccgtc cgctcgcgaa attgtgtcag acgcaccaac atcctttttc
     7081 tgtcattcag gtcgatcttg accattttaa agcgattaat gaccgctttg gtcatcaggc
     7141 gggcgaccgt gttctttctc atgctgccgg attaattagc agttccttgc gtgcgcagga
     7201 cgttgccggg cgggtcggtg gtgaggagtt ttgtgtgatt ctgccaggcg cgagtctgac
     7261 ggaggctgcg gaagtcgcag aacgtattcg cctgaagtta aatgaaaaag agatgttgat
     7321 tgctaagagt acgacgatac gcatcagtgc ctcgttgggg gtaagtagca gcgaggaaac
     7381 cggtgattat gattttgaac aactccagtc actggccgac cgtcggcttt atctcgctaa
     7441 acaggctggg cgcaatcggg tattcgcgag cgataacgct taacgggcgg agaaaaaatg
     7501 gtccagccct tcacgccatc cttccggtcc ttcacgctgc gttcgccaga cgcgggccgg
     7561 atcctcatca tgcagatgca ccccttcacg gtttagccct ttcacaatca ccgcgtaatc
     7621 cattacctcc agtaagggcg catcgtttgg cccatcgccc aggccaagtg tggttgggcg
     7681 tttgcctgac aattgttgat aggtcgcgat aatccagttg gcagcctgat cttttccggc
     7741 agaggcatcc aggacgtgcc agaagcgcgc accttgcata aactgtaagc ccagttcgtt
     7801 cagacgagcg gtaaattgtg ccatacgctc gtcactgtcg cgccagatta gcgttaccga
     7861 cgcctcatga agctgcgtca gcgccgcctg gctacggctt aatcccgtcc attcggcgat
     7921 ggttgcatcg tcgacatcat caaaagtcgt gaatttaaaa tgttctttct cgcgtagcgt
     7981 atttaaaacc tggctgattt cgccatggct aatacctgag atgatgcgtg gaaaaccgtc
     8041 tatctcctgc cattgttcag caagctggat cactgcgcca ttctctgcaa tcagcggtaa
     8101 accttgtagc cccaacattt tttgcaagta cagcatttcc gctgatgttt tactgctaca
     8161 gagaatgacg ggaacatttg cttcgcgtaa acggctgagc cagggggctg ccggttgcca
     8221 gtcataacta tgactgtcca gcagggtgcc atcaagatcg ctaaaaacca gtagtggttg
     8281 ttgaattgaa aacatagtcg cgcagtactc cccttaccag gatgattctc aaaacagcac
     8341 aaattatcaa agatgatttt ttcgggagcg atcccgctga cgccagagcc accaaggggt
     8401 gacgtgcgtc acatttctat tcataagtag cgttaatcat tcatatagcg aatattttct
     8461 tgtcagcgaa aaaaattgcg gataaggtga tgaacacatc agatttcctg gtgtaacgaa
     8521 ttttttaagt gcttcttgct taagcaagtt tcatcccgac cccctcaggg tcgggatttt
     8581 tttattgtgc attcaacgat tcacttcaga gacgctgaag tcatgaatat ccagttcgaa
     8641 ggcatccgcc aattcacgcc atgtatgata ttcacgtcca tcgacgctaa cgtgttcaga
     8701 atcgttctgg ctgcgatgaa cttcgctttc gctaatttca ttgatcgccg ccagcagggc
     8761 atcgacatcg acgctgacct cacgttttgc ggtatcgctg tactcttttg cggttttcat
     8821 catctttcct cgcaaccgtt actctgttgt tgccgccagt cgttaagtcc gcctggcatc
     8881 tcttaagtat agaccgtcga gaaaatcagc actcgcgcgc gcctggcgct gccagaaaaa
     8941 cgaaattgtt ctacactggc acaaagccac aggaggaaaa cgatgaaggt gaatgatcgg
     9001 gtaacagtca aaacggatgg cggtccgcgt cgtcctggcg tggtactggc agttgaggag
     9061 tttagtgaag gcacaatgta cctggtttcg ctggaagact acccgctcgg catctggttc
     9121 tttaatgaag cagggcatca ggacggtatc tttgtggaga aagcagagta atctaattca
     9181 gcctgactgg tgggaaacca ccagtcagaa tgtgttagcg catgttgaca aaaataccat
     9241 tagtcacatt atccgtcagt cggacgacat ggtagataac ctgtttatta tgcgttttga
     9301 tcttacgttt aatattacct ttatgcgatg aaacggtctt ggctttgata ttcatttggt
     9361 cggagatttg aatggttccc tgacctgcca tccacattcg caacatactc gattcggttc
     9421 ggctcaatga taacgtcggc atatttaaaa acgaggttat cgttgtctct tttttcagaa
     9481 tatcgccaag gatatcgtcg agagattccg gtttaatcga tttagaactg atcaataaat
     9541 tttttctgac caatagatat tcatcaaaat gaacattggc aattgccata aaaacgataa
     9601 ataacgtatt gggatgttga ttaatgatga gcttgatacg ctgactgtta gaagcatcgt
     9661 ggatgaaaca gtcctcatta ataaacacca ctgaagggcg ctgtgaatca caagctatgg
     9721 caaggtcatc aacggtttca atgtcgttga tttctctttt tttaacccct ctactcaaca
     9781 gatacccggt taaacctagt cgggtgtaac tacataaatc cataataatc gttgacatgg
     9841 cataccctca ctcaatgcgt aacgataatt ccccttacct gaatatttca tcatgactaa
     9901 acggaacaac atgggtcacc taatgcgcca ctctcgcgat ttttcaggcg gacttactat
     9961 cccgtaaagt gttgtataat ttgcctggaa ttgtcttaaa gtaaagtaaa tgttgcgata
    10021 tgtgagtgag cttaaaacaa atatttcgct gcaggagtat cctggaagat gttcgtaggt
    10081 attatttttc ggataatcct taggataaca tgataaacgt tacggaatta tattaatggc
    10141 aattcactaa taatatcagc cagcaaatta aaaatttcac tgaataaatg ttcgcaaaaa
    10201 ggcgcaatta acggcattaa tgccgccatt aaagagatgc cgacagtcag agttaatgga
    10261 aatccaataa caaaaatgga taattgcggg gccatacgat taagtaaacc taatgccaga
    10321 ttcagtgtca gcagcagagt aatgagcggt aacgccagca tcagcccgtt aaggaaaatc
    10381 aaactccctg ctttggtgag tgccagaaac gcattgctgt tcaacggttc gccaccaatc
    10441 ggcagggtgt gaaaggtatc gaccagcagt gaaatcaacc ataaatgacc gttaaatgtc
    10501 aggaacagca gtaacgccag catatccatg atacgcgcta aaacgggcat attaagatgg
    10561 ctggccggat cgacaaacgt cgcaaatgac agccccattt gcagaccgat aatttcgcca
    10621 gcggttcgca cagcggcaaa ggcaaattgc atggtaaaac caagcgcaat gccgatcagg
    10681 atctgctgca cggccagcca cagagcaaag aacgaaaaaa caggaacatc gttggcaggt
    10741 aatgatgggg caatggcgaa cgtgatcatc attgccagac ccagttttac ccgtttcggt
    10801 acgctgcgtt cgctcagaat cggcgcggtg gagatcagcg ccagcacgcg cagtaacggc
    10861 cagaagtaca ggcttaacca ggatagccat tgttcgcttg tcacctgcaa catagtacgg
    10921 ctacccgatg atatacggca ggttagtgaa caaggtgcgg acgtaatcca gcaacagatt
    10981 gagcatccac ggtccggcaa taataatggc gataaatacg gcgatgattt tcggaataaa
    11041 cgacagcgtc atttcgttaa tctgcgtggc ggcctgcaaa atactgatga taaggcccgt
    11101 gaccaacgct accaacaata gcggggcagc cagtgccagc gcgactttca tcgcttcagt
    11161 ccccatcatc atgaccgatt caggtgtcat tttgcctctc tagctgtaaa agctctgcgc
    11221 cagcgaaccg accagcaatt gccagccatc caccagtaca aacagcatca gtttaaaggg
    11281 cagagcaatg gtggcggggg gaaccatcat catccccaat gccatcaaca cgctggctat
    11341 caccaggtcg ataatcaaaa aagggatgaa aatcgtgaag cctatctgaa atgcggtttt
    11401 caactcgctg gtcacgtagg ccgggagcaa aatgcgcatc ggcacggctt caggtccctg
    11461 caacgggccg gtattcgcca gtctggcaaa caaccctaaa tctgcctcac gggtctgacg
    11521 cagcataaac tcacgcagcg gctgcgcccc tttttccagc gcctcctgca ttgatatttt
    11581 ctcttcgctg aatggctggt acgcatcgac ataaattttg tcgatcaccg gtgacataat
    11641 aaaaaaggtc aaaaacagtg ccagccccag caatacctgg ttaggtggcg cggagggtgt
    11701 tcccagcgcg ttacgcaata aaccaaaaac aatgatgatg cgggtgaaac tggtcatcat
    11761 cagtaaaatt gccggaataa acgtcaacga ggtgatgaac accagcgtct gcaccgggag
    11821 cgaccagctt tgtccaccgc caggcagcgg ctggctggtg atacccggca gttgcgcgaa
    11881 ggcgaggggc gtaatcagcc agagaaggac aggtgcgaca gacaataaac gacgcatcag
    11941 gatctcccgc tacgcttaag caaatttttc atgaccgact gaaaatcggt ctgcggtatc
    12001 tcttctgttg gtgctgaagg gggaagttta tgcagcagat tgatttgccc tgcggtaacg
    12061 ccgagcacca gccgtgcatc ttccacatcg accaccacaa cccgttcacg cgcgcccagc
    12121 gaagcactgg cgctaatttt cagaccgtta acgccagtgc gtttcggggc aaatcccaac
    12181 cgttttacca gccaggcagc agcgaggatc agggcaataa tggcgatgag tgcgccgctc
    12241 acctgcagca gtggcgcagc agaaaccggc gcggaagatt gcacagtagc gtggttattc
    12301 atcactaacg gctcaggcgg cgcattcgct cagacggagt aatgatatcg gtgatccgca
    12361 cgccatattt atcggcaacg accaccactt cgccctgggc gattaaataa ccgttgatca
    12421 gaatatccag tggttcgccc gccagaccgt ccagcgccac gacggaccct tgcgtcagac
    12481 gcaacagctc tttgatggtc attcgtgtgc ggcccagctc gacggtcagc ttgaccggaa
    12541 tatccataat caggtcgata tcctgcaacg ttccgctgac atcaccaccg ccaaattgct
    12601 ggaacaccgc gtcggcagcg cttttgctgc tggttgattt ttgttcgctc aacgcttcag
    12661 cccacagatc gtccattgcg ccgttgttgt catcggccgg attattcatg tcactcattt
    12721 gggctgttcc tcgttcagag aatttaaaat cgggttaatc aaatgttcta tccgtaacgc
    12781 atactgaccg ttgagagtgc cgtactgact ggtcagcacc gggacgccgt caacatgggc
    12841 gatgatgcgg tcgggttttt ctatcggcag gacgtcaccg ggtttcagtt ttaaaatctg
    12901 cgacaggcgt agcgagatat cggcaaagtt ggcgaccagc tccagctgtg aatgctgcac
    12961 ctggcgcacc aggttatcgc gccagttctg atcttcatta cgcgagtttt ccagcggcgg
    13021 gttaaccaac aattcccgta gcggctcgat catgctgaat ggcaggcaga tattaaattc
    13081 gccggtcagg ttgccaatct ccacatggaa cggcgtgtta accacaatgt cgttcggcga
    13141 ggtggtgata ttggtaaatt tcacctgcat ttccgaacgc acgtactcaa cttccagcgg
    13201 attaatcgcc ttccatgcgt cgctatagcc ttcaagcgcc agtttcaaca tgcggttgat
    13261 gacgcgctgt tcggtatggg taaactcgcg accttccact ttggtcggga agcgtccatc
    13321 gccgccaaac aggttatcca cggcgataaa caccagactc ggtgagaaca ccaccagccc
    13381 agtgccgcgc agcggtttta gatggataag gttcaggttg gtcggcaccg gcaggttgcg
    13441 ggcaaattca tggtacggct gaatgcggat ggccccgacg gttatatccg ggctacgacg
    13501 cagcaggttg aacagcccca tacgaaaatg gcgggcaaag cgctcattaa tgatttccag
    13561 cgcctgcaaa cgttcgcgca caacccgtcg ttgggtattc ggatcgtacg gacgaatgtc
    13621 actttcgccg ctaacactgg ctgtcggttc gtctttgact tcgctgtcac cattcaacag
    13681 cgcatcaatt tcagcttgag aaagaatact atcgcccatg tcgttatcgc agaataaaag
    13741 cggtatacag cacgtcggtg acatcctgtt tcggttgccc ggcaacaagc ggggtggaaa
    13801 gtgtggtttt aatctcggca atcaggtttt tcttgccttc ttctgtcgcc agtacggcag
    13861 catcctgacg cgaaaacagt aacagcaaac ggctacggac ttccggcaaa tactcactca
    13921 gccgcgagcg ggtagcttca tctttcaggc gcagggttat gccgatataa agcacgcgat
    13981 ccgcatcgcc cagattgacc gtaaaggtat ccagcgcata gaagaccggg gagggcacga
    14041 cgcgttgctg cgctttgtcg tcagcagcaa cctgatgcga atgccagtag ctgtaacctg
    14101 cgctggcaca ggccgcgagg gtaatgaata ccagaatcgg gatccaaagc gatcgcttgc
    14161 ttttcttgct tatcgcgtaa tcagtcatgt gttgcggtct tcctgtgtcg ctactgctta
    14221 tcggctaatt atcctgcgtc ttgagcgcaa caaagcgggg aaaagacgcg gattacggtg
    14281 ctacctctga cgttaggcga aaatatcaac gccgctgttg cctgttacgc gcccttgtaa
    14341 agagacggga accggaagcg tatcgtcgtc ttcccccgcc agaggttcat ggtttactgt
    14401 gcgttggctt tgctgttgtt gggaagcggc ctgctgctga ccactaaagc tttcgccact
    14461 gatgttgctt tgccctaact gaatgccact ttcggccagc tgcgtacgca gtaccggcag
    14521 cgctgcttcc agggcggcgc gtacatgctg atgcgatgaa accatctgga tttgcgcctg
    14581 gttatcatcc actttgaggg agatttgcac ttcacctaaa tcctgcgggt gcagacgcaa
    14641 ctctgcactt tgttgcccct ggcgggtgaa cagcgaaata tgctggctta atgattgttg
    14701 ccattcgtga gaacccagcg gtgcactcaa aacaggcgcg gcgacggtgg gcagtggctg
    14761 tgtctggtgt ggagtgatta gcgggctggc ggcagcagtc accggcgaag gcgtgctgat
    14821 gacttccgct ttactctggg cttctgctac ctgcggtgtt aatggctgag ctggtgtgcc
    14881 gggggcatca tcaggctgtg ctgttgtgag ttgcgcagaa gtcagttttg tgaagagcgt
    14941 tggtttctct gccggtaaca cggttgacgg cacatcagtc actttgggcg tattgtcaaa
    15001 acccggcaac atcgcaaaaa gggcgctcag gcttgcggtg acgtcttcat tcagatcatc
    15061 cgctttttcg tcttttgtcg tatttttgtc agccaccgca gccaacgcca tcgtctgagc
    15121 ggtggttaac ggtgttgatg tggattgttc gtcgttgatg acaggcggtg tttcatccac
    15181 agggatcagt aaattagctt gttgcgcgtc ggaaacaata tcgctgatca gcggctcgcc
    15241 ttttgtcgtg ggcttatctg ttgccaccag caactggggg gccgctttgt cggtagttgt
    15301 ctcgcctgct aatgcttcgc tcaacaacgc gagaaaatct tgtgcagcat cgctggcttt
    15361 gccgccaggc aatgtggtgg tgtcaacgtc ggcggtaatc aagggcgcta agcgaatcat
    15421 tcaggtttcc tcatggcggc gcgctgggcg aactcatcca tctttttctg atcgaggcgg
    15481 ttttctgcaa gcagtgccgc cgtggattgc cgttcctgca gtgtctgcca ggcctgcaaa
    15541 cgttgtttct tttctcgcca gctgttcagg gcaatgtcaa ctttctgcgt ccactgatta
    15601 agttgctggc gatgctgagt aatggctttt tccagcgtct ggataaactg ctgatagttg
    15661 atccagcggt tgctggtcat cccggcactc atatcgctgt tgaggttatt gcgatattca
    15721 ttctgataat cgatcagcat tttgagctgt tcttccgcct gctgacaccc gcgacgcatt
    15781 tcacccagca ggcgcgcggc atcctctacc tctttttctg ccagatcttt cagggtcgcc
    15841 agcgcgccat gttctgccat ctgccgttat ctcctgggtt atgacactgt cgggaaaata
    15901 cgctccagcc cctggagaga cgcttcccag tccgcgcgtt caaaaatgcc ttgttgcaaa
    15961 tagccctcca gctgcggcca cagggcgatg gctttgtcga gcatcggatc gctgcctttg
    16021 gcatacgcgc cgacgctaac cagatcgcgg ttacgctgaa aactcgacaa cagctgtttg
    16081 aaggtgcgca ctcgcgcgta atgttgctca ctgatcaacg ccgtcattgc gcggctgatc
    16141 gacgcttcaa tatcgatagc cggatagtgc ccggcttccg ccagtcggcg agacagcaca
    16201 atgtgaccgt cgaggatcgc ccgcgcggag tctgcaatgg ggtcctgctg gtcatcgcct
    16261 tcagtaagca cggtataaaa cgcggtaatc gagccgccgc cgctaatgcc atttccggca
    16321 cgctcgacca gtgccggtaa tttggcaaac accgacggtg gataaccttt ggtggcgggt
    16381 ggttcgccaa tcgccagcgc aatttcacgc tgggccatcg cgtagcgggt gagggagtcc
    16441 ataatcagca acacatgctg accacgatcg cgaaaatctt cggcaatgcg cgtggcatag
    16501 gcggcacctt gcattcgcag gagcggagac acatccgccg gagcggcaat caccactgat
    16561 cgtgcacgcc cttcggcacc gaggatattc tcaataaaat cttttacttc gcgcccacgt
    16621 tcaccaatca aacccacgac aatgacatcg gcgcgggtgt aacgtgccat catccccagc
    16681 agcacacttt taccaacgcc ggacccggca aacagcccca tacgctgccc acgcccaacg
    16741 gtaagcaggg cattgattgg gcgcacgccg gtgtccagca catgttcaat cggtgtacgt
    16801 tgcaacgggt taaatggcgg ggtaatcagc gcaccggttt ccgtcgtatc gggggagggc
    16861 aggccatcga gcggtttacc gctgccgtcc agaacgcgac ctaataacgc cggaccgagc
    16921 ggcaactgct tgccgctttg cagtccttca gccgaaatgt ttttggcata aacccgcgcg
    16981 ccgggcagga caccttccac ttcctccagc ggcattaaaa acagccgttg accgttaaag
    17041 ccaacgactt cgctttctac ttcgtgcgtt tcgctgccgt tctggcgttc aatgacacag
    17101 gttgcgccga gcggcaattg taatccggtg gcttccagca ccagcccggt agcgcgggtt
    17161 aatcgcccgt agcgacgtac cgcaggcaac tgcgccattt tggcttcaaa gttatccagc
    17221 gtggttagcc agcgagtcag gcgcgtggtc attacaccac tcctggtgct gccagacggc
    17281 agagttcttg ccagcgagtc aggcgcgtgg tcattacacc actcctggtg ctgccagacg
    17341 gcagagttct tgccagcgag tggcgacact ggcgtcgaga tcgccttcat cggcggagac
    17401 tttacagccg ccaggatgga gggtgggatc gccccgcaag cgccagccat gcaaacttaa
    17461 ggtagcgccg agcatatcat ccacacgttg cagatcatcc gggtgcacgc gcagctgtgg
    17521 tttaccgctg aataacggtt cttgctgcaa caactgttgg atctgtttga tcagtgccga
    17581 gttatccacc gttggcgtct gaccgatgac ctgacgtgcc gcctccagcg ccatctgcat
    17641 caggcgcgac gctatcacac tatcaagtgc atcaagggta gtttgaaatt cgctgaccag
    17701 ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc gcctctgcca gaccttgctc
    17761 cagcccctgg gccagtcctt cctgatagcc ctgcttatga ccttgctggc gaccttcggc
    17821 aatacccgcc tgataacctt gctcatgggc ctgcatttgc agttgcgcca gttgctgctc
    17881 aaggctgggt tcagcctctt caatgatggt ttcttccgac tcgaccatgg gcacaaactc
    17941 tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc cagggcagat tatcagacat
    18001 aggtatcctc gccgctgcca attaccatct cgccagtttc ggcaaggcgg cgcacaatca
    18061 gcagaatcgc tttctgttcg ttttccacct gcgacagacg caccggacca cggttggcga
    18121 gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt gcgcaggaat ttctcgcgca
    18181 gtggctgctc ggcccctttc agcgcgatca acagcgattc ggaatccact tcctgcaaca
    18241 gacgctgaat gctgcgatcg tcgacatcca ccagattttc gaacaggaac atctcgtcga
    18301 tgattttctg cgccagctcg ccgtcgaatt cacgcacggc ggtaataacg gcttcttcct
    18361 gctgagtttt catcaggttg ataatttcgg ctgccgttct cacgccgccc attttgctgc
    18421 gcttgagatt ctgaccgtcg agcaagccat tcagtacttc ggtcagttcc gccagcgcgg
    18481 ctggctgcac gccgccaaag gtggcgatac gcaacatcac gtcgtggcgc agacgttcat
    18541 cgaacaacgc cagaatatcg gcggcctggg cgcgcttcag atgcaccaga atggtggcga
    18601 taatttgcgg atgctcatca cgaatcagat cggcggcgct ctgcggctcc ataaagttga
    18661 gcgtttcaat accgctggcg gtatcgcgag tttcgagaat atcttccagc aggctggcgg
    18721 cacgttcctc acccagagct ttgaccaata ccgaacgcag ataatcgttg gcgttgatat
    18781 tcagtgcggc aaactgttca gcttcttgct caaactccgc cagcacatcg gttagctgct
    18841 tgttggagat ctgcgtgacg ttcgccattg cagcgctcag ggtttgcacc tcgcgctggg
    18901 agaggtgctt gaacacttct gccgcccggt cttcgccaat ggtcatcagc aggatgacgc
    18961 ttttatcggt gcctgtcagg ttactcatga tcgttattta tccactggcg aatgaccagc
    19021 gccaccacgc gcggatcgtt atcagacatt tcacggatac gctggctcat gacttctgcc
    19081 cccagacgtt ggttagcgcg ccgttgttgg agttgttcgt ctttgctcag gcggacttcc
    19141 accgcatctt ccacttcctc gcgggcctgc gcctgttgct gtacagcttt catcgcctca
    19201 gcgcgacgtg ttagctgcgg gcgtaccgct ttacgccaca gcagccacgc caccagcagt
    19261 accagcaacc agcgaccggc agccagtaac tgatcgataa acgcttgctg ttgccagaac
    19321 ggcagttctc cgccgctttc gtcactacta ttgaacggcg agttaacgac attgagcgag
    19381 tcaccgcgtt tttcagaaaa gcccatcgcc tcgcgggtca gatcttcaat ttgtttcatc
    19441 tgttcgttgc tgagaggcaa tggtttaccg tctggcaagg ttttgtaatt caccactacc
    19501 gcgactgaca gacgttgcac atcgcccacg ttcattttgg tatgacgaat agtgcgatcg
    19561 acttcgtagt tactggtttc attccgctgt gtgctacgcg gcccactatt gctggtggtg
    19621 ctcgcctgct gctggcggtt attttgattt gccggaggcg tgctgattgg cgcgttattc
    19681 gcaggagccg gttgattcga caacgccccc ggtacgccgc ccggataacc ggaaccgctt
    19741 tgctcgctct cattaagctg gcgtgaacga agcgccgcct gagattcatc accattaggg
    19801 cgatactgtt cttccgtttg ttctttactg gcgaagtcca gttgcgccgt gacctgggcg
    19861 tgaatattac cgttaccaac aataggcgac aggattgctt caatacgccg ctgaatacgg
    19921 ccttcgacat cgctggcata tttcaattga gcgtcattaa gatcgcgcca gctggtattg
    19981 gactgggtta acagatgtcc gccctgatcc accagcgtga cgtttcccgg cggcagacca
    20041 gcaacggcgc tggaaaccag atgcacaatg gcgctaattt gcccttcatc gagtgcgcgg
    20101 ccgggtaaca gatttaccgt caccgatgca gaaggggatt tttgttcacg gacgaataaa
    20161 gacggtttcg gcattgccag atgtacgcgc gcccctttta ccgggccgat agtttcgatg
    20221 gtacgagaaa gctcgccttc cagcgcccgc tgataattca cctgttcgct gaactggctg
    20281 ataccaaact tttcctgatc gagtagttcg aaaccgaccg cgccgccttt gggcaaacct
    20341 tgttgcgcca ggcgcaggcg cagctcgtga actttatctg ccggaacttc aatagcgccg
    20401 ctggcttcgc tgaagcggta aggaatattc atttgcgtca gttggctgac aattgcgcca
    20461 ccatcctgat cggaaagatt gctgaataat gtgcggtagt cgggggcttt cgcccacagg
    20521 atcagtgcga ccatgaccgc cactgccgcg gaaccggcaa caatcaatgg aattttcgga
    20581 ttcgcacgca ggcgattaag ccactcaaga gattttgtct gggctgcagt cgcattcatc
    20641 gcgcacctcg tggctgatta tggacggcgt tattggcaaa gtggaacaag actcactccc
    20701 gggtcagcaa actcgaaaaa ttgacgctcc cattatttgt gctttcgttt ttttctatcc
    20761 gccaataaac ccgttttttt gttgctattt agcgcctttg tcttattgac ttactggtag
    20821 gctttgctac cagaaattat ccaggagatg aaaatgtcag cgatacaggg gattgaaggg
    20881 gttatcagcc agttacaggc tacggcgatg agtgcgcgtg cgcaggaatc actgccgcaa
    20941 ccgaccatta gttttgccgg gcagctgcac gccgcgctcg atcgcataag cgatacacaa
    21001 acagccgccc gcacgcaggc agaaaaattc actctcggtg aacccggcgt ggcgttaaac
    21061 gatgtgatga ccgatatgca aaaagcctca gtttctatgc aaatggggat tcaggtgcgt
    21121 aataagctgg tggcggcata tcaggaagta atgagcatgc aggtataggt tgtgtaacct
    21181 gttgttaatg acatccgatc tcaccgcgtg gggcatggat ggggcaaact tactcattat
    21241 ctggtttgaa atggatacct gatcctgaaa gttttctgcc atccacgatc cgtatacacg
    21301 ataaaccccc tgaacatcag agtgtctcat ttgacacatc accacgtcgg atgttgttac
    21361 gaccacagac gcaacagtaa cctctggata attttatctt tgttgatgtt tttattcatg
    21421 ttccgggcta tggatatgag cgatttttga tagtggtaat tttattgtgt tttagaatgc
    21481 gccctgggca atataattta ttaaacagga gtgtaaatgt ttccattaaa tgatctgtcg
    21541 ttaaaaactc agtcggttca attaaataaa atcacatcga atactgagtc tacgataaaa
    21601 caacacgagt tagtatctga tgatgcaatc ataaatgaat tatcaagtga gttagtcagt
    21661 tgtcttggaa atgataagtt tacaccagtt agtgaagaca gcaacttact gaatatgctg
    21721 tctgaattta agttattgag agagcaatgt ttcaggtggg gtaattatac tctattgttt
    21781 gaaaattatg gggcttatga taagacggga tctatcacga tagaaaaaag tcagggggag
    21841 gggactttac ccattcggca taaattagag tttatatcga ccaatattgc agagttgctg
    21901 gacaagttaa ccaaaattac agatgccagg ctttgcaaag gtttcagtga ctgggctagt
    21961 tcagtcaaag aaggcgcatc gaatgacttg aaagaaaatg tggatagagc attggtgaga
    22021 atgtttaaat gtgttaagct tcacagtaat gaacttaact tatcaagcct ttctttgggt
    22081 tctgtgccgc ctcttcctga gtggattgaa atgcttagcc ttgtttataa tgaacttgat
    22141 tcaatacagg tgcccgaatc gtgcaaagaa ttagaactcg atttcaataa ccttacagaa
    22201 tttccacaag tacctgatgg aattaccctg atctccgtaa ataataacct gatatcgtat
    22261 attgactcat ttccgccaaa ggctaagaaa atttttattt gtcacaataa gctatcggaa
    22321 ataccagcac taccagacac cgctaaggtt tttgattgta gtgagaataa tattaaagaa
    22381 attagatggt tccccaaaaa tttgaaagaa gcgtatattg aatataataa gattgaggtt
    22441 attcctgcga tacctggcaa tttaaaatta ctttgtatga aatgtaatcc tattaaagag
    22501 gcat
//
LOCUS       DABSYT020000072        22492 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.072, whole genome shotgun sequence.
ACCESSION   DABSYT020000072 DABSYT020000000
VERSION     DABSYT020000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.072"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            251..1057
                     /gene="xthA"
                     /locus_tag="ID725_RS16440"
                     /old_locus_tag="pgaptmp_003288"
     CDS             251..1057
                     /gene="xthA"
                     /locus_tag="ID725_RS16440"
                     /old_locus_tag="pgaptmp_003288"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGV7851819.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            1224..1934
                     /gene="ydjX"
                     /locus_tag="ID725_RS16445"
                     /old_locus_tag="pgaptmp_003289"
     CDS             1224..1934
                     /gene="ydjX"
                     /locus_tag="ID725_RS16445"
                     /old_locus_tag="pgaptmp_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGV7851820.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            1939..2616
                     /gene="ydjY"
                     /locus_tag="ID725_RS16450"
                     /old_locus_tag="pgaptmp_003290"
     CDS             1939..2616
                     /gene="ydjY"
                     /locus_tag="ID725_RS16450"
                     /old_locus_tag="pgaptmp_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGV7851821.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            2631..3338
                     /gene="ydjZ"
                     /locus_tag="ID725_RS16455"
                     /old_locus_tag="pgaptmp_003291"
     CDS             2631..3338
                     /gene="ydjZ"
                     /locus_tag="ID725_RS16455"
                     /old_locus_tag="pgaptmp_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HGV7851822.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            3338..3886
                     /gene="ynjA"
                     /locus_tag="ID725_RS16460"
                     /old_locus_tag="pgaptmp_003292"
     CDS             3338..3886
                     /gene="ynjA"
                     /locus_tag="ID725_RS16460"
                     /old_locus_tag="pgaptmp_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGV7851823.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            3896..5062
                     /gene="ynjB"
                     /locus_tag="ID725_RS16465"
                     /old_locus_tag="pgaptmp_003293"
     CDS             3896..5062
                     /gene="ynjB"
                     /locus_tag="ID725_RS16465"
                     /old_locus_tag="pgaptmp_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGV7851824.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            5035..6570
                     /gene="ynjC"
                     /locus_tag="ID725_RS16470"
                     /old_locus_tag="pgaptmp_003294"
     CDS             5035..6570
                     /gene="ynjC"
                     /locus_tag="ID725_RS16470"
                     /old_locus_tag="pgaptmp_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGV7851825.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            6570..7223
                     /gene="ynjD"
                     /locus_tag="ID725_RS16475"
                     /old_locus_tag="pgaptmp_003295"
     CDS             6570..7223
                     /gene="ynjD"
                     /locus_tag="ID725_RS16475"
                     /old_locus_tag="pgaptmp_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGV7851826.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNTRRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            7290..8597
                     /gene="ynjE"
                     /locus_tag="ID725_RS16480"
                     /old_locus_tag="pgaptmp_003296"
     CDS             7290..8597
                     /gene="ynjE"
                     /locus_tag="ID725_RS16480"
                     /old_locus_tag="pgaptmp_003296"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HGV7851827.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(8606..9226)
                     /gene="ynjF"
                     /locus_tag="ID725_RS16485"
                     /old_locus_tag="pgaptmp_003297"
     CDS             complement(8606..9226)
                     /gene="ynjF"
                     /locus_tag="ID725_RS16485"
                     /old_locus_tag="pgaptmp_003297"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGV7851828.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            9313..9720
                     /gene="nudG"
                     /locus_tag="ID725_RS16490"
                     /old_locus_tag="pgaptmp_003298"
     CDS             9313..9720
                     /gene="nudG"
                     /locus_tag="ID725_RS16490"
                     /old_locus_tag="pgaptmp_003298"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGV7851829.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(9686..9958)
                     /gene="ynjH"
                     /locus_tag="ID725_RS16495"
                     /old_locus_tag="pgaptmp_003299"
     CDS             complement(9686..9958)
                     /gene="ynjH"
                     /locus_tag="ID725_RS16495"
                     /old_locus_tag="pgaptmp_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGV7851830.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            10194..11537
                     /gene="gdhA"
                     /locus_tag="ID725_RS16500"
                     /old_locus_tag="pgaptmp_003300"
     CDS             10194..11537
                     /gene="gdhA"
                     /locus_tag="ID725_RS16500"
                     /old_locus_tag="pgaptmp_003300"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGV7851831.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(11654..12694)
                     /gene="ynjI"
                     /locus_tag="ID725_RS16505"
                     /old_locus_tag="pgaptmp_003301"
     CDS             complement(11654..12694)
                     /gene="ynjI"
                     /locus_tag="ID725_RS16505"
                     /old_locus_tag="pgaptmp_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGV7851832.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKTMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(12822..14783)
                     /gene="topB"
                     /locus_tag="ID725_RS16510"
                     /old_locus_tag="pgaptmp_003302"
     CDS             complement(12822..14783)
                     /gene="topB"
                     /locus_tag="ID725_RS16510"
                     /old_locus_tag="pgaptmp_003302"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGV7851833.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(14788..15831)
                     /gene="selD"
                     /locus_tag="ID725_RS16515"
                     /old_locus_tag="pgaptmp_003303"
     CDS             complement(14788..15831)
                     /gene="selD"
                     /locus_tag="ID725_RS16515"
                     /old_locus_tag="pgaptmp_003303"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGV7851834.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(15948..16499)
                     /gene="ydjA"
                     /locus_tag="ID725_RS16520"
                     /old_locus_tag="pgaptmp_003304"
     CDS             complement(15948..16499)
                     /gene="ydjA"
                     /locus_tag="ID725_RS16520"
                     /old_locus_tag="pgaptmp_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGV7851835.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            16660..18516
                     /gene="sppA"
                     /locus_tag="ID725_RS16525"
                     /old_locus_tag="pgaptmp_003305"
     CDS             16660..18516
                     /gene="sppA"
                     /locus_tag="ID725_RS16525"
                     /old_locus_tag="pgaptmp_003305"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGV7851836.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            18632..19342
                     /locus_tag="ID725_RS16530"
                     /old_locus_tag="pgaptmp_003306"
     CDS             18632..19342
                     /locus_tag="ID725_RS16530"
                     /old_locus_tag="pgaptmp_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HGV7851837.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            19474..20490
                     /gene="ansA"
                     /locus_tag="ID725_RS16535"
                     /old_locus_tag="pgaptmp_003307"
     CDS             19474..20490
                     /gene="ansA"
                     /locus_tag="ID725_RS16535"
                     /old_locus_tag="pgaptmp_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGV7851838.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            20501..21142
                     /gene="pncA"
                     /locus_tag="ID725_RS16540"
                     /old_locus_tag="pgaptmp_003308"
     CDS             20501..21142
                     /gene="pncA"
                     /locus_tag="ID725_RS16540"
                     /old_locus_tag="pgaptmp_003308"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGV7851839.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSALFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(21235..>22492)
                     /gene="ydjE"
                     /locus_tag="ID725_RS16545"
                     /old_locus_tag="pgaptmp_003309"
     CDS             complement(21235..>22492)
                     /gene="ydjE"
                     /locus_tag="ID725_RS16545"
                     /old_locus_tag="pgaptmp_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7851840.1"
                     /translation="GFLSYSGNVVLAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGG
                     FIGDYFGRRRAFRINLLIVGIAATGAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFT
                     EFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSG
                     KYFIESPRWLAGKGQIAGAECQLREVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFW
                     LLFKGEMLRRTLVAITVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAP
                     VGIFIAALIIDHFPRRLFGSTLLIIIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVC
                     FASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVML
                     LLCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT         5452 a         5844 c         5722 g         5474 t
ORIGIN      
        1 ggtgaataaa aagaataaaa aacgcaatgt tatgcagaag taaaatataa ttctggaatt
       61 gtgatcattg acgaaattta ctggaaatta ctgcgccatt ctgacgcagc gcgcaccaaa
      121 agcgggcatt ttttgcgcca tcgttgacat cattaacaac catcgatcaa atcacttaac
      181 aacaggcggt aagcaaagcg aaattctgct accatccacg cactctttat ctgaataaat
      241 ggcagcgact atgaaatttg tctcttttaa tatcaatggc ctgcgcgcca gaccccacca
      301 gcttgaagcc atcgtcgaaa agcaccaacc ggatgtgatt ggcctgcagg agacaaaagt
      361 tcatgacgat atgtttcccc tcgaagaggt ggcgaagctc ggctacaacg tgttttatca
      421 cgggcagaaa ggccattatg gcgtggcgct gctgaccaaa gagacgccga ttgccgtgcg
      481 tcgcggcttt cccggtgacg aagaagaggc gcagcggcgg attattatgg cggaaatccc
      541 ctcgccgctg ggcaatgtca ccgtgatcaa cggttacttc ccgcagggtg aaagccgcga
      601 ccatccgata aaattcccgg caaaggcgca gttttatcag aatctgcaaa actacctgga
      661 aaccgaactc aagcgtgaaa atccggtgct gattatgggc gatatgaata tcagtcctgg
      721 cgacctggat attggcattg gcgaagagaa ccgtaagcgc tggctgcgta ccggaaaatg
      781 ttctttcctt ccggaggaac gcgaatggat ggagagactg atgagctggg gattggttga
      841 taccttccgc catgcgaatc cgcaaacagc agatcgtttc tcatggtttg attaccgctc
      901 aaaaggtttt gacgataacc gtggtctgcg catcgacctg ctgctcgcca gccaaccgct
      961 ggcagaatgt tgcgtagaaa ccggcatcga ctatgaaatc cgcagcatgg aaaaaccgtc
     1021 cgatcacgcc cccgtctggg cgaccttccg ccgctaattt agcagctctc ctggctcaaa
     1081 ctgggtcagg agaattaacc ttgagaaaaa tcaacaaact gtcagtaatg atttgttgcc
     1141 tgccgtcctt tgttataccg tcactgcgtt tttagttgtc tgaccacttc tctattatca
     1201 agtttgatat aggaaactcc acgatgaacg ctgagcgtaa atttcttttt gcctgtctta
     1261 tttttgcgct ggccatttac gctatccacg ctttcggttt attcgatctg ctcaccgatt
     1321 taccccactt acagacactc attcgccaga gcggattgtt cggttatagc ctctatattc
     1381 tgttattcat cattgccacc ctctttctgt taccaggaag catattggtg atcgcgggag
     1441 gaatagtttt tggcccgttc ttagggacac tactctcatt aattgccgcc acgctggcct
     1501 cctcgtgctc attcctgctg gcgcgctgga tgggacgtga tttactgctg aaatacgttg
     1561 gccatagcca taccttccag gccattgaaa aaggcattgc gcgtaacggt attgattttc
     1621 ttattctgac ccgcttaatc ccgttgtttc cttacaatat tcaaaattac gcttacggat
     1681 taaccacaat cgccttctgg ccttataccc ttatttcggc actcacgacc ctgcccggta
     1741 ttgttattta taccgtgatg gcaagcgatc tcgccaatga aggcattacg ctgcgcttta
     1801 ttttacaact ctgtctggcg ggcctggcgc tgtttattct cgtccagctc gcaaaactct
     1861 acgcccgaca caaacatgtg gatctgtctg cttcgcgccg caacccactt actcacccaa
     1921 aaaatgaagg atagaacgat gttgcaacat tattcagtgt catggaaaaa aggactgact
     1981 gcactctgct tactggctgt tgctgggctt agcggctgcg atcaaaaaga gaatgcggcg
     2041 gcaaaagtgg aatacgacgg actttcgaac agccaaccgc tgcgcgtcga tgccaataac
     2101 catacggtaa ccatgctggt gcaaattaat ggtcgtttcc tcaccgacga cactcgtcac
     2161 ggtattgtgt ttaaagatgg ctccaacgga cataaatcgc tgtttatggg ttatgcgacc
     2221 ccgaaagcat tttatgaagc cctgaaagag gcaggtggta caccgggcga aaacatgacg
     2281 atggataata aagaaacgac tcatgtcaca ggcagcaaac tggatatttc ggtcaactgg
     2341 caaggggcgg caaaaacgta ttccttcgat gaagtgattg ttgacagtaa tggcaagaaa
     2401 ctggacatgc gctttggcgg taatttaacg gcagcagaag agaagaaaac gggttgcctg
     2461 gtgtgtctgg atagctgccc ggtcggcatc gtcagcaatg caacatacac ttatggtgcg
     2521 gttgaaaaac gaggtgaagt taaattcaaa ggcaatgcct cggttctccc ggcgaataac
     2581 acgctggcaa cggttacctt taaaatcacc gaataaagcc aggataaagg atgatgaaga
     2641 tgcaatcgcg taaaatctgg tactaccgta taaccctcat catcctgttg ttcgctatgc
     2701 tgctggcatg ggcgctgctt cccggcgtcc atgagtttat caatcgcagc gttgcggcgt
     2761 ttgccgccgt ggaccaacag ggtatagaac gctttattca gtcttacggc gcactggcgg
     2821 cagttgtctc gttcttgttg atgattttac aggccattgc tgcaccgcta cctgcgtttt
     2881 tgatcacctt tgccaatgcg tcgctgtttg gcgcgttctg gggcggctcg ctgtcgtgga
     2941 ccagttcgat ggccggcgcg gcactgtgct tttttatcgc cagagtgatg ggccgcgaag
     3001 tggtggaaaa attaaccggc aaaaccgtac ttgacagtat ggacggcttt ttcactcgct
     3061 acggcaaaca caccatcctg gtctgtcggt tattgccttt tgtccctttc gatccaatca
     3121 gctatgctgc cggtttgact tcaatacgtt ttcgctcgtt ttttatcgcc accgggcttg
     3181 gtcagttacc ggcgactatt gtttattcct gggcgggcag catgttaaca ggcggtacct
     3241 tctggtttgt caccggactg tttattctgt ttgccctgac cgtggtgatt tttatggcga
     3301 agaaaatatg gcttgagcgc cagaagagga atgcctgatg ggtttaccgc cgcttagcaa
     3361 aattcctttc attttacgtc cgcaggcgtg gctgcatcgt cgccattacg gcgaggtgct
     3421 aagccccatt cgctggtggg ggcggatccc gtttatcttt tatctggtgt cgatgtttgt
     3481 tggctggctg gagcgcaaac gctcaccgct cgatccggta gtacgatcgc ttgtcagcgc
     3541 gcgcattgcg caaatgtgcc tgtgtgagtt ttgtgtggat atcaccagta tgaaagtcgc
     3601 cgagcgcacc ggcagcagcg ataaactgct ggcagtggct gactggcggc aaagcccgct
     3661 ctttagcgat gaagaacggc tggcgctgga gtacgccgaa gccgcaagcg taacgccgcc
     3721 aacggtcgat gatgccctgc gtacccgact ggctgcgcat tttgacgctc aggagctcac
     3781 cgaactgacg gcattgatcg gcctgcaaaa tctgtcagcc cgttttaatt ctgccatgga
     3841 cattcccgct caggggctgt gccgtattcc ggaaaaacgt tcttaaggag agatgatgcg
     3901 ccattgtggg tggttgctgg gattgttatc gctgttttct ctggcaacac atgccagtga
     3961 ctggcaagaa attaaaaatg aggccaaagg gcaaactgtc tggtttaacg cctggggcgg
     4021 cgataccgca attaaccgct atctcgactg ggttagcggc gagatgaaaa cccattacgc
     4081 tataaacctg aagattgttc gtctggcgga tgccgcagac gcggtgaagc gcattcagac
     4141 cgaagctgct gccggacgta aaacgggcgg ctcggtggat ctgctctggg tgaacggcga
     4201 aaacttccgc accttaaaag aggccaattt actgcaaacc gactgggcag agactctgcc
     4261 caactggcgc tatgtcgaca cacagctgcc ggtgcgggaa gatttttcag tgcctacaga
     4321 aggggctgaa tcgccctggg ggggcgcaca actgacattt atcgcccgcc gcgatgttac
     4381 gccacagcca ccacaaacgc cgcaagcctt actggagttt gctaaagcca atcccggcac
     4441 ggttacctat ccgcgcccac cggactttac cggcacggcg tttcttgaac agttgctgat
     4501 tatgctgacg cccgatcccg ccgcattaaa tgaagcgccg aacgatgcga ctttcgcccg
     4561 tgtcactgct cccttgtggc aatatcttga tgcgctgcat ccgtatttgt ggcgcgaagg
     4621 aaaggatttc ccgccttcgc ccgcgcggat ggatgctctg ctgaaagccg gaacatttcg
     4681 cctgtcgctg acctttaacc ccgcgcatgc gcagcaaaaa atcgccagcg gcgatttgcc
     4741 tgcaagcagt tacagttttg gctttcgcga ggggatgatt ggcaacgtgc atttcgtcac
     4801 cattcctgcc aacgcgaatg ccagtgctgc ggcgaaggta gttgccaatt tcttgctctc
     4861 acccgatgcg caattgcgta aagcagatcc cgctgtctgg ggcgatcctt ctgttctcga
     4921 tccgcaaaaa ctgcctgatg ggcagcgcga aacattgcaa tcaagaatgc cgcaagatct
     4981 gccgccggta ctggctgaac cgcacgcagg ttgggtaaat gcgctggaac aagaatggct
     5041 acaccgttac ggtacgcatt aatctttttg ctgtgggcga tggtggcggt gatttatgca
     5101 ccgctgatcc cggcagctct cacgctgata tcgcctgctt tgtcgttgac acactggcag
     5161 gcgttatttg ccgatccaca gttaccgcag gcattactgg caacgctggt gtcgacaacc
     5221 atcgcggcgg tcggggcgtt gttgattgcc ctactggtga ttgtggcgct gtggcctggg
     5281 ccgaaatggc agcgtatgtg cacccgtctg ccgtggctgc tcgccattcc ccatgtggct
     5341 tttgccacca gcgcccttct gctctttgct gacggagggc tgctttatga ctatttcccg
     5401 tatttcactc cgccaatgga caaattgggt attgggctgg gcttcacgct ggcggtgaaa
     5461 gaaagcgcat ttctgctgtg gatcttagca gcggtattga gtgaaaaacg gcttttacag
     5521 caagtcatcg tgctggattc gctgggctac agccgctggc aatgcctgaa ctggctgctg
     5581 ttgccctccg tcgcgcctgc gctggcaatg gcgatgctgg cgattgttgc ctggtcgctg
     5641 tcggtcgtag atgtggcgat tattcttggg ccaggtaatc caccgacgct ggcagtaatt
     5701 agctggcagt ggttaactca gggcgacgcc gatcaacaaa cgaaaggcgc actcgccagc
     5761 ctgctgctga tgttgttact cgccgcctac gttttgctgg gctatctgct atggtgtacc
     5821 tggcggcgta ctattccccg cgtagatggc gttcgtaagc ctgccacgcc tttattgcca
     5881 ggcattacgc tggcgagttt tttacccttg accagtgtgc tgtgtgtggt tctgctggcg
     5941 atcctcgcgg atcagtcgac gatcaatagt gaagcgctca tcaacagcct gacaatgggg
     6001 ctggtggcga cattcatcgc tttgctcctg ttactgctgt ggctggaatg ggggccacag
     6061 cgtcgccagt tgtggctatg gttgccgatt ttattaccgg cactgccgct ggtggcgggc
     6121 cagtacacgc tggcgctatg gctgaacatg gatggaagct ggacggcggt ggtctggggg
     6181 catctgctgt gggtaatgcc gtggatgctg tttatcctgc aacccgcctg gcagcgcatt
     6241 gattcacggt taattttgat tgcacaaaca ctgggctggt cgcgggccaa aatcttcttt
     6301 tacgtgaaat gtccgcttat gctacgccct acgctgattg ccttcgcggt gggattttca
     6361 gtcagtattg cgcagtatat gccgacgctg tggctgggcg cggggcgttt tccgacgctc
     6421 accactgagg cggtggcatt aagcagcggc ggcagcaacg gtattctcgc cgccctggct
     6481 ttatggcaac tgctattacc gcttattatt tttgccctga ccgcgttagt cgcaaaatgg
     6541 gtaggttatg tcagacaagg actccgctaa tgctctgcgt gaaaaatgtt tcgctacgtt
     6601 taccagaaag ttgcttgctg acaaacgtta actttacggt ggataaaggt gacattgtca
     6661 cgttaatggg gccgtctggc tgtggaaaat ccactctgtt ttcatggatg attggtgcac
     6721 tggccggaca gttttcttgt acaggtgagc tatggctcaa tgagcaacgg attgacatgc
     6781 tacccaccgc acagcgtcag attggcattc tttttcagga tgcactgtta tttgaccagt
     6841 tcagtgtcgg gcaaaattta ctgctggcgc taccggcgac acttaagggg aatacccgac
     6901 gtaatgccgt gaatgatgca cttgagcgtt caggccttga cggagctttc catcaggatc
     6961 ctgctacttt gtctggcggt cagcgagcgc gcgttgctct gcttcgcgcc cttctcgctc
     7021 agccaaaagc gttgctactg gatgagccat tcagccgtct tgatgtggct ctgcgcgata
     7081 attttcgcca gtgggtgttc agcgaagttc gcgaactggc gatccccgtc gttcaggtaa
     7141 cgcacgatct ccaggatgtt cctgctgata gttctgttct ggatatggcg cagtggtcag
     7201 aaaattacaa caaactgcga taacgcaaag tttttctcaa tgcgtcagtt cagaatggcg
     7261 cactcaaaac tacaatgtcg ggattttcga tgaaacgtgt ttctcaaatg accgcgctgg
     7321 caatggcttt agggctggct tgcgcttctt cgtgggccgc tgaactggcg aagcctctta
     7381 cacttgacca gcttcaacaa caaaatggca aagcgataga tactcgctcc agcgcgtttt
     7441 ataacggctg gccacaaacc ttaaatggcc cttctggtca tgaacctgcc gccttaaacc
     7501 tctctgctag ctggcttgac aaaatgagca ccgaacagct caacgcgtgg atcaagcaac
     7561 ataacctgaa agccgatgcc ccggtggcgc tgtacggtaa tggcaaagat gtcgacgccg
     7621 tcaaaacgcg actgcaaaaa gcaggtttta cgcatatctc cattctgagt gacgcgctaa
     7681 gcgaaccttc ccgtctacaa aaactgccgc actttgagca gctggtttat ccgcaatggc
     7741 tgcacgacct gcaacaaggt aaagaggtta cggcgaaacc tgccggtgac tggaaagtca
     7801 ttgaagcggc caggggcgct cctaagcttt accttatcag ccatattccc ggtgctgact
     7861 acatcgatac caacgaagtg gaaagcgaac cgctgtggaa caaagtttct gatgaacaac
     7921 taaaagcgat gctggcaaaa cacggcattc gccatgacac cacggtcatt ctgtacgggc
     7981 gtgacgtata cgctgcagcg cgtgtggcgc agattatgct ttatgccggc gtgaaagatg
     8041 tgcgcctgct ggatggcggc tggcaaacct ggtccgacgc gggtctgccc gttgagcgcg
     8101 gtacgccacc gaaagtgaaa gcggaaccgg atttcggcgt gaagatcccg gcacaaccgc
     8161 agttgatgct cgatatggaa caggcccgtg gactgctgca tcgccaggat gcatcgctgg
     8221 tgagcattcg ttcgtggcca gaatttatcg gtacgaccag cggttacagc tatattaaac
     8281 caaaaggtga aatagccgga gcacgttggg gacacgctgg tagcgactcg acgcatatgg
     8341 aagatttcca taatccggat ggcaccatgc gtagcgccga tgatattacc gctatgtgga
     8401 aagcatggaa tatcaaacca gatcagcaag tttcattcta ctgcggcacc ggctggcgcg
     8461 cgtccgaaac ctttatgtac gcacgagcaa tgggctggaa taacgtctcc gtttacgacg
     8521 gcggctggta cgaatggagc agcgatccaa aaaatccggt agcaaccggt gaacgcggcc
     8581 cggacagcag caaataacat tgcaattact gacgctggag cgacttcagc gtcagatacc
     8641 cactccacac ccgcgtaaag gtcgtcatcc agcacaacgc accgaagatc cacgcaaacc
     8701 acgcaaacca tgcagggaat aaacatccca gcacaaacag taagatcgtt tctgtgcctt
     8761 cggttaatcc ccccagataa taaaacgatt tatgcgcata accgggatta tcaatctgat
     8821 atttcgccgc cagcgcagca aaagcgagaa aactgctacc cgtgccgata aacgcaaaca
     8881 gcagccagcc gcctgccagg gcgttttgct gcggtgcggc gaggataaac ccaaaaggta
     8941 ccagcgcgta aaaaagaaaa tcgagagaaa tatcaagaaa gccgcccgcg tcggtaagcc
     9001 ctctgcgccg cgccagcgca ccgtccagac catcaagcag cctgttcaac aaaatgacga
     9061 caagcgccgc cagataccag cccagcgcca gaaaaggcag tgccagcaca ccgatggcaa
     9121 atcccactaa tgttaaacca tctggcgtaa tgcccggttt atcaagaacc cgcacacact
     9181 gatgcaacaa cggtttaatc cggggatgaa gatggcggtc tagcacaggc actccttaaa
     9241 tataaagcct ttctgattga gcaacagtgc ggatattatg gcatttttcg cttatctgcc
     9301 cgtgtgtaat ttatgaaaat gattgaagtt gttgccgcca tcattgaacg tgatggcaaa
     9361 attttactcg cgcaacgccc cgcccagagc gatcaggcgg gattatggga gtttgccggt
     9421 ggtaaagtcg agccggatga aagccagcag caggcgctgg tgcgtgagtt aaacgaagaa
     9481 ttgggcatcg aagcaactgt gggtgagtat gttgccagcc atcagcgaga agtttcgggc
     9541 cggattatcc atcttcatgc ctggcacgta cccgacttcc acgggacgtt acaggcacat
     9601 gaacatcagg cgctggtctg gtgctcacct gaagaggcgc tgcaatatcc gctggctcct
     9661 gctgacattc cattattaga ggcgtttatg gctttacgcg ccgccagacc agcggattag
     9721 tgctaagggt tttgtcatcc cgctggcatt gcagcagtat tccttcggct ttaattaccg
     9781 ccccttcaga ataattttta tcctgataaa cgcagcactg agtacagggc tgcgctgact
     9841 gcccgcctga actgaccact tctggcggta cgtttacctc cacgtccgga ccataatgcg
     9901 ggttagccag tgcaattaat ggaaatgcta atactacggc gaacaatgct cgactcacag
     9961 ggaactcctt aacgttattg tctctgctac tgataacggt agccgggtgg caaaacttta
    10021 gcgtctgagt tatcgcattt ggttatgaga ttactctcgt tattaatttg ctttcctggg
    10081 tcattttttt cttgcttacc gtcacattct tgatggtata gtcgaaaact gcaaaagcac
    10141 atgacataaa caacataagc acaatcgtat taatatataa gggttttata tctatggatc
    10201 agacatattc tctggagtca ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg
    10261 agttcgcgca agccgttcgt gaagtaatga ccacactctg gccttttctt gaacaaaatc
    10321 caaaatatcg ccagatgtca ttactggagc gtctggttga accggagcgc gtgatccagt
    10381 ttcgcgtggt atgggttgat gatcgcaatc aggtacaggt caaccgtgca tggcgtgtgc
    10441 agttcagctc tgccatcggc ccgtacaaag gcggtatgcg cttccatccg tcagttaacc
    10501 tttccattct taaattcctc ggcttcgaac aaaccttcaa aaatgccctg actactctgc
    10561 cgatgggcgg tggtaaaggc ggcagcgatt tcgacccgaa agggaaaagc gaaggcgaag
    10621 tgatgcgttt ctgccaggcg ctgatgactg aactgtatcg ccacctgggc gcggataccg
    10681 acgttccggc aggtgatatc ggggttggtg gtcgtgaagt cggctttatg gcggggatga
    10741 tgaaaaagct ctccaacaat accgcctgcg tcttcaccgg taagggcctt tcatttggcg
    10801 gcagtcttat tcgcccggaa gctaccggct acggtctggt ttatttcaca gaagcaatgc
    10861 taaaacgcca cggtatgggt tttgaaggca tgcgcgtttc cgtttctggc tccggcaacg
    10921 tcgcccagta cgctatcgaa aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag
    10981 actccagcgg cactgtagtt gatgaaagcg gattcacgaa agagaaactg gcacgtctta
    11041 tcgaaatcaa atccagccgc gatggtcgag tggcagatta cgccaaagaa tttggtctgg
    11101 tctatctcga aggccaacag ccgtggtctg taccggttga tatcgccctg ccttgcgcta
    11161 cccagaacga actggatgtt gacgccgcgc atcagcttat cgctaatggc gttaaagccg
    11221 tcgccgaagg ggcaaatatg ccgaccacca tcgaagcgac tgaactgttc cagcaggcag
    11281 gcgtactatt tgcaccgggt aaagcggcta atgctggtgg cgtcgctaca tcgggcctgg
    11341 aaatggcaca aaacgctgcg cgcctgggct ggaaagccga gaaagttgac gcacgtttgc
    11401 atcacatcat gctggatatc caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa
    11461 ccaactacgt gcagggcgcg aacattgccg gttttgtgaa ggttgccgat gcgatgctgg
    11521 cgcagggtgt gatttaagtt gtaaatgcct gatggcgcta cgcttatcag gcctacaaat
    11581 gggcacaatt cattgcagtt acgctctaat gtaggccggg caagcgcagc gcccccggca
    11641 aaatttcagg cgtttatgag tatttaacgg atgatgctcc ccagggaaca tttcttatgg
    11701 gccaacggca tttcttactg tagtgctccc aaaactgctt gtcgtaacga taacaagctt
    11761 caagttcagc atccgttaac tttctgcgat agcagcagat atgccagtaa agaaatccca
    11821 ttagactatt tttttgataa tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag
    11881 caagcattat ataatgccag gccagttctt cttcaattgt cccgttttga aaagctgtgc
    11941 ttgatatcga gatcatccat gataattccg ccgcccatat tagcttcgcc gaggatttac
    12001 cggagctatg attagcgcaa tcagagatat agtctgaggg aaaaacagca aatttattca
    12061 acaaggcgat aacctgctct ggggcttcct ccatgtttgc tttaaaggta ttggctccat
    12121 ggtcgccaga aagaaaatgc tccattaagg cacaataact ttcgctatct tcgatacccc
    12181 attgttcctc taaagactcg cgtcttttac ttatgatatc gatcgagtca aaaggaagca
    12241 catgatattg gaaggtatct ttgccaggtt caggctttcg cggccagaac tccagcgttt
    12301 cagaccattg cttatgatag aatcgataag gtgcgatcaa ttgtagcgcc tgtaatttct
    12361 cgatactgag cggctcaata cctttagcct gataataatg cagctgttct ttttttgctt
    12421 taaaaccggc ccgaacaata agccccatca taattaatag ataaagaaaa gagcatcccg
    12481 cggtaatcag gcctctttca ttcaaaccgt tggatgttat cgctgcgaat acaaacatta
    12541 cagcgacaac acatgttaaa taaaaccccc acttacaaag cagcatggtc ttattttctt
    12601 taatcatccg ttcaaaatta ctattaaata tttcccagcc attaaaagaa tacttctcgc
    12661 tcccaggatg gttttgtaat aaaacttttt tcatcacatt ccctgttatt acatacttta
    12721 gaggcgataa ttttatccat gcaaaaaaaa tatccaacga attttcttga tccgctggat
    12781 atttcagaat atgactcgaa tagcacgaaa gattcgctcg cttacgctat cgccccgctt
    12841 ccggcttcat ctgctggcgg actttttttc gcactacgtt tacgcggtgc agcctttttc
    12901 ttatcagcac tgccaccact gcccggagcc acaatgccgc gaaactgccg caccggagta
    12961 cgtttggctt gatcaataag ctgatatagc gtccccacca gcggctgcat aaagtcctga
    13021 tagcgacact gcttttcgct gatttgcgtc agcaccgatt cccagtgcgc ggtcatgtcc
    13081 ggtcgcgtag ccatttctgg cagcgaatgg aataacgctt ttccggcgtc ggtggagtgg
    13141 atatagcgcc cttttttgat gaggaaacca cgcttgaaca acagttcaat aatcccggca
    13201 cgcgttgctt ctgtccccag accatcggtc gcacgaagga tctttttcag atctttgtcc
    13261 tgcacaaagc gcgcgatccc ggtcatcgcc gaaagcagtg ttgcatcggt aaaatggcgc
    13321 ggcggctggg tttgccgctc taccacttca cctttttcac acagcaactc atcgcctttc
    13381 gccaccacag gcaatggcgt accgtcgttt tcttcatcgc gctctttgct gcctaacagc
    13441 gtgcgccagc ctgcttcagc aagaaaacgc gctttagcga caaatttgcc tttggcaatg
    13501 tccagttcga taacacactt gcggaacacc gcatccgggc agaactgcat cagatactga
    13561 cgggaaatca ggttatagac cttcgcttcg ttctccgtca ggttgatcgc agaactccgt
    13621 gcggtcggaa tgatggcgtg gtgcgcatcg acctttttgt catcccaaca gcggttgcgt
    13681 atatctggat ctaccactgg ctgcggcaac agatctggtg catgaacgct gatggcattc
    13741 atcaccgcgt ggcgtccggc aaaatgttct tctggcaaat agcgacaatc ggaacgcgga
    13801 taagtgatta gcttgtgcgt ttcgtacagc ttctggcaga tatcaagcac gttctgcgcg
    13861 ctcagaccaa agcgttttgc cgcttcaatc tgcaacgccg aaagcgaaaa cggcagcggc
    13921 gcggattctg attcccgttt atcgttatag ctggtgacaa tagccggttg accactaatg
    13981 cggttaacca catgctccgc cagtggacga tgtaacaagc gcccttcttc atcctggtac
    14041 ggttcacacg cttcgctcgg ttgccagata gcggtaaacc gctcatcggc aggtgtcacg
    14101 atatgtgctt tgacttcaaa gaagtctttc gccacgaagt tttcaatctc ttcatcgcgg
    14161 cgcaccacca gcccaagcac gggcgtctgc acgcgtccca cggaaagcac gccctgataa
    14221 ccggcattgc gaccgagaat ggtatacgca cgggtcatat tgatgccgta cagccagtcg
    14281 gcacgcgctc gcgccagcgc agaaacgcac agcggtacaa actcactgtt ggaacgaaga
    14341 cggtcgatcg cccgctcaac cgcctgcggg ttcaggtcgt ttatcaagca acgctgcacc
    14401 tgctggcgct tttccggtgc cagttgtaga tagtccagca cttcatccac cagcagttgc
    14461 ccttcgcgat ccgggtcccc ggcgtgaacg atttcgctgg cttcatgcag gtaccgtttg
    14521 atgacgttaa gttgtttggt cacggagggt cggggctgta attgccactt ttccgggaca
    14581 atcggcaaat ccgcaagatt ccagcgcgca tagcggctgt cgtaggcgtc tggctgcgcc
    14641 tgctcaagca ggtgaccgat acaccaggtc accacctgac cattaccgca ctcgataaag
    14701 ccatcgcctt tccggtgcgg tttgggcagg acatcagcaa tggcgcgcgc cagactcggt
    14761 ttttcggcaa taaacaaccg cattgaatta acggatctca accatggcac gaccgccgcg
    14821 tgctggcacc agttcgccaa tcgccgtcag ttcaatgcca aactcggcgg ctgtagcttt
    14881 gacctcattt tcggcttccg gcatgaccgc cagcagcaaa ccgccagaag tttgcggatc
    14941 gcacagcaaa tcgcgcactt cacgcggcat ttcacccatc agatgaccgt agctggcaaa
    15001 gttacgttcg gtgccgccag gcactgcacc caacttaatg tactcttcaa cccccggcaa
    15061 tttcgggatc gcgtcatagt caacgcgcgc ctgcacacct gcgccctgac acatttcgct
    15121 caaatggccc agcaggccaa agcccgttac gtcggtcatc gcttttacgc cttcaatgtt
    15181 ggcaaaggac gcgcctgcga tgttcatccg gcacatcact tccgtcgcca gtccctgatg
    15241 ttctggtttc agtagtgatt ttttctcagc cgtggtaaga acgccgatcc ccagcggttt
    15301 cgtcaggaac agtttacatc cggcttgtgc ggtactgttt ttcttcaccc gctcggtcgg
    15361 tacgatcccc gttaccgcca gaccaaaaat cggctccggc gcatcgatgg agtgaccgcc
    15421 agccagcgca atacccgcct gacggcatgc atagcgtcca ccttcggtta cttcgcgggc
    15481 aatttccggg gaaagtttgt taatcggcca gccgaggatc gcaattgcca taatcggttt
    15541 gccccccatc gcgaagatat cgctgatggc gttagtcgcc gcaatgcggc caaaatcgaa
    15601 aggattatca acgatcggca taaagaagtc ggtggtactg ataacgctgg tgccattgcc
    15661 cagatcgtac accgccgcat cgtcgcgggt ttcattaccc acaagcaaat tcggatcaac
    15721 aaacttcgcc tgctcactgt gcaggatggt ttccaacact tttggggaaa ttttacagcc
    15781 gcaaccagct ccgtggctgt attgggtcaa acgaatcgag ttctcgctca tggacatctc
    15841 ctgtcaatgc aatccgggta tggtaaccct cattccgtga agtgataagt gagagtgtct
    15901 gaattcctgc gcctttgctc acaatccaga cagtttcgcg acaattatca gaaataagtg
    15961 acaaacggcg tcgggtccgg gacgtttatc gacgtagatg ctttcagctg cggcgtgccg
    16021 aggtagagaa aaccgacaat tttatcctgc tcacggcaac cgaatgcttc acgcactacc
    16081 ggactttcag ttaatgcgcc actgcgccag atgccgccaa acccctgggc aacggctgcc
    16141 atttgcatcg ccatgaccgc gcatccggca gacatttcct gttcccatcg cgggacttta
    16201 tgattttctt cgcatttcgc caccaccgtg atgatgagcg gtgcgcggaa cggcgcatta
    16261 cgggctttgt cgatagcttt gtcatcacta ccggcagcaa tcgccccctg ttccagtacg
    16321 gcgctgaaac gctcgcgccc ttccccttca atcacaaaaa aatgccacgg ttgcatggac
    16381 ttatggtccg gcgcgcgcat acccgcacgc aggatgtttt gcagttgttc acccgttggt
    16441 gcgggttcag ccaggcggga ggcgctacgg cgattgatca atagttcgag tgcatccatt
    16501 tgattaactc ctgtcgtgat atttattcac aaaattaaca cgagagtgga ttttgttaca
    16561 gcacagtccg caattcctgc tgacaagtgc cggttgggtc attacgataa ccacatctat
    16621 tgcgcctgtg acaggtgtga ccttaagttg ggagaataca tgcgaaccct ttggcgattt
    16681 attgccggat tttttaaatg gacgtggcgt ctgctgaatt tcgtccgtga aatggtactt
    16741 aacctgttct ttatttttct cgtgctggtt ggtgtgggga tctggatgca ggtcagtggt
    16801 ggtgattcga aagaaacggc cagtcgtggt gcactgctgc tggacatttc tggtgtgatc
    16861 gtcgataaac ccgacagttc tcagcggttt agtaaattaa gccgccaact acttggtgcc
    16921 agttccgatc gtctgcagga aaactcactg tttgatatcg tcaataccat tcgccaggcg
    16981 aaggatgacc gcaatatcac cggtatcgtg atggatctga aaaacttcgc aggcggcgac
    17041 caaccgtcta tgcagtacat cggcaaagcc ctgaaagagt ttcgtgacag cgggaaaccg
    17101 gtttatgccg ttggcgagaa ctacagccag gggcaatatt atctcgccag tttcgccaat
    17161 aaaatttggc tgtcaccgca aggcgtggtg gatttgcacg gttttgctac caacggtctg
    17221 tactacaaat cgttgctgga taagctgaaa gtttccaccc atgtgttccg cgtgggtacg
    17281 tataaatctg ccgttgaacc gtttattcgt gatgacatgt caccggcagc ccgcgaagct
    17341 gacagccgct ggattggtga gctgtggcaa aactatctga atactgttgc cgctaaccgg
    17401 cagatccctg ctcagcaggt attccctggc gcgcaagggt tgcttgaggg tttaaccaaa
    17461 accggtggcg ataccgcgaa atatgcactg gaaaacaagc tggtcgatgc actggcatcg
    17521 agtgcggaaa tcgaaaaagc actgaccaaa gagttcggct ggagtaaggc tgataaaaat
    17581 tatcgcgcca tcagttatta cgattacgca ttgaaaacgc cggcagatac cggtgacagc
    17641 atcggtgtcg tcttcgctaa tggcgcaatt atggatggcg aggaaactca ggggaatgtt
    17701 ggcggtgata ccactgcggc acaaatccgc gacgctcgcc ttgacccgaa agtgaaagcg
    17761 attgtcctgc gtgttaatag cccaggcggc agcgttaccg cgtctgaagt gattcgcgct
    17821 gaactggcag cagcccgggc agcgggtaag cctgtggttg tatcgatggg cggcatggcg
    17881 gcatctggtg gttactggat ttccacgcca gctaattaca ttgtggctaa ccccagcacc
    17941 ctgaccggtt ctattggtat cttcggcgtg atcaccaccg tagaaaatag tctggattcg
    18001 attggtgttc atactgatgg tgtctcaact tcaccgctgg cggatgtttc tatcaccagg
    18061 gcactgccgc cggaagcgca gcagatgatg cagttaagca ttgagaatgg ctataaacgc
    18121 tttatcacgc tggttgctga tgcgcgtcat tcgacgccgg agcagattga taaaatcgcc
    18181 cagggccacg tctggaccgg tcaggacgca aaagctaacg ggctggtcga tagtctcggg
    18241 gatttcgatg atgcggtcgc caaagcagca gagctggcaa aagcgaaaca gtggcatctg
    18301 gaatactacg ttgatgaacc gaccttcttc gacaaagtga tggacaacat gtctggttct
    18361 gtccgggcaa tgttgccaga tgcgttccag gccatgttac ctgcaccgct ggcctcggta
    18421 gcctctaccg ttaaaagtga aagcgacaag ctggcggcgt ttaacgaccc acaaaaccgt
    18481 tatgcgtttt gcctgacctg cgccaacgtg cgttaaatcc tgcaaaaatg tggccgatat
    18541 gctgtcggcc acactcctga atcggactgt ctttcactcc ctctctccac tacattccct
    18601 tgctcattta gcatctgata tggagtcatc agtgaacaag gccccctctt taattgccgc
    18661 tatcgtcctc ggactgggga ttagcgcctg tggttatttt gttggcgatg gtgttaaaca
    18721 tttaaaaacc aacaaccgtt atgtcaatgt gcgcgggctt tctgaaaaag aagttcgcgc
    18781 agatacggcg gaattaacaa ttgcgattaa ctttaagggt aatgttcccg gcgaactgtt
    18841 cccgaaactg gaagaagcgc agaaaaaaat tgttgccgaa ctcaatgctc aggggattaa
    18901 cgagaaagag ataattctgg gccaatggac aagcaagcga acagattcct tttatctgaa
    18961 agatgatccc acaatgccac gctataacgc agacgggtca gtgacgataa aaacccataa
    19021 tgttgctgct gtcgaaaaag tcgtcgcgaa attaaacgaa ctgcaagtcg ctacggatgg
    19081 cgctatcgct gagagcaaag tcgcctatcg ctttaatggt attggcgcac tgcgcgctga
    19141 gatgattgct gccgccacca aagatgcacg caacgccgca ctacaatttg caacggactc
    19201 aggtagccag gtggggtcaa ttagcgatgc ctcacaaggc gtattccaga tctttgccag
    19261 tggcagtgat gaagatgatc ctacggcgat taataaaacg gtacgcgtag tcacgaccgt
    19321 aacctatgcg ttgcaggatt aaccactttt ctccggcggg acactaatcc ctccgtcctg
    19381 ccggactttt ctcatatcca cagagtatcc aggtgcgctc gctgcccctc acgtatatac
    19441 ttttgctctt tcgatatcat tcatatcaat atcatgcaaa agaaatcaat ttacgttgcc
    19501 tacacgggcg ggaccatcgg gatgcagcgt tccgagcagg gttatatacc ggtgtcaggt
    19561 catctacaac gccaactggc gctgatgccg gaattccatc gcccggagat gccagatttc
    19621 accattcatg aatatacgcc gctgatggat tcttcagata tgacgccgga agactggcag
    19681 catattgctg aagatattaa agcgcactat gacgactatg atggttttgt cattctgcac
    19741 ggcaccgaca cgatggcgta taccgcctct gcgctgtcgt tcatgctcga aaatctcggt
    19801 aaaccggtca ttgtgacagg gtcacaaatc ccgctggctg agttacgctc tgacggacaa
    19861 attaatctgc tgaatgcgtt gtacgttgcg gcgaattatc cgatcaacga agtaacactc
    19921 ttttttaata accgactgta tcgcggcaac cgcactacca aagcccatgc cgatggtttt
    19981 gatgcgtttg cctctccaaa ccttcctccg ttactggaag caggtatcca tattcgtcgt
    20041 ttgaatacgc cacccgcccc gcacggtgaa ggggaattga tcgtacatcc aatcacccca
    20101 caaccaattg gcgtagtgac gatttatcca gggatttctg ctgacgtcgt gcgcaatttt
    20161 ctgcgccaac cggtgaaagc attgattctg cgctcctatg gcgtgggtaa tgcgccacaa
    20221 aacaaagcct tcctgcagga attacaagaa gccagcgatc gcggtattgt ggtggtcaac
    20281 ctgacacaat gtatgtccgg taaagtgaat atgggtggtt atgccaccgg taacgccctc
    20341 gcccatgctg gcgttattgg cggtgcagat atgactgtag aagccacgct aaccaaactg
    20401 cattacctgc ttagccagga actggatact gaaaccattc gcaaggccat gagccaaaac
    20461 ctgcgcggcg aactgacgcc ggatgattaa ggagactgca atgccccctc gcgccctgtt
    20521 actggtcgat ttacaaaatg atttctgtgc tggtggcgcg ctcgccgtgc cggaaggtga
    20581 cagtacggtg gatgtcgcta accgcctgat tgactggtgc cagtcgcgcg gtgaagcggt
    20641 tatcgccagt caggactggc acccggcgaa tcacggcagt tttgccagcc agcacggtgt
    20701 agagccttat acgccaggcc aactcgacgg tttgccacaa accttctggc cagatcactg
    20761 tgtgcagaac agtgaaggcg cacaattaca tccgttactg aaccaaaaag cgatcgcagc
    20821 tgtgttccat aaaggcgaaa atcctttagt tgacagttac agtgcccttt ttgataacgg
    20881 ccgtcggcag aaaacctctc tcgatgactg gttacgcgat catgaaatcg atgaattgat
    20941 cgttatgggc ctggctactg actattgcgt gaagtttacc gtgctggacg cgttacagtt
    21001 aggttataag gtaaacgtga ttaccgatgg ttgtcgtggc gtgaatatcc agccccagga
    21061 cagtgcgcac gcgtttatgg agatgtcagc agctggggca acgctatata cgctggcaga
    21121 ctgggaagag acgcaggggt aattttacgc tggcctacaa ttctgtactg gcattgtagg
    21181 ccaaataaaa cacgtcagtg gcacatctgg caattgatgc catcaacgaa agatttaatt
    21241 tacctcagaa atctcttcca acgacacctt ccgcgtttcg atgccaaaaa tggagagaac
    21301 cagcgcacag agcaataaca tgacaccaag taccataaac accgtgatcg acccataatg
    21361 tgttaataat gccgcaacgc catagggcgt gaagactgcg acgatccgtc cgacggcatt
    21421 aacgaaaccc gaaccgcgca ggcgtaaatg cgttggccaa agctccggga tataaaccgc
    21481 cgacgcgaag caaacataca tgtataaaaa gaagatcatc accaggccat agattaaaat
    21541 cgcccactct gtagtctgaa ttgaatagat atagcctaac actgcaataa taataagtaa
    21601 ggtggagcca aataaccgac gaggaaaatg atcaataatt aatgccgcaa taaatattcc
    21661 taccggagcg ccaatcataa taacagcggt cattaatatt gatttatcga catcaatgcc
    21721 ggagttaaca aatatggtcg gtatccatac ggtgatggta taaagcgaaa tgttcattgc
    21781 aattaaaaca gtaatcgcga ctaatgtacg tcgtaacatt tcacctttaa acaggagcca
    21841 gaaagtaccc ttgattactt taaccttgct gttgctctga tacgaagtaa gcgggggtaa
    21901 acgaatactc ttctctcttt caatttgctg ctctacttca cgaagttggc attctgcacc
    21961 tgcgatttgc cctttccctg ccagccatcg tggcgactca ataaagtatt tacctgagag
    22021 aaaccaggct aacagtatgc caataccacc caacagaaac attatccgcc aactaaaaaa
    22081 agcgataacc accacaccta tcgccgcaga cagcatgggc gaccagttac caacaaatga
    22141 gagccgcgcg gaccattttc cacgcaccgt cgcggggata aactccgtaa atgaggcata
    22201 gccaaccata atcagcgccc ccattcctgt tcccatcagg aagcgaaaga agataagcca
    22261 gtacatatca gggacaaaag cggcccctgt tgcagcaata ccgacgatga gaagatttat
    22321 gcgaaacgcc ctgcgccgcc caaagtaatc accgatgaac ccaccggtaa gagagccgat
    22381 gaaataacca aacattaatg ccgaggtaaa ggcggcattg aggaaattat ttgaccatcc
    22441 attgcttacc agctttgcta agacgacatt accggagtaa ctcaaaaacc ct
//
LOCUS       DABSYT020000073        22448 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.073, whole genome shotgun sequence.
ACCESSION   DABSYT020000073 DABSYT020000000
VERSION     DABSYT020000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.073"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            334..740
                     /gene="ipbA"
                     /locus_tag="ID725_RS16550"
                     /old_locus_tag="pgaptmp_003310"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             334..740
                     /gene="ipbA"
                     /locus_tag="ID725_RS16550"
                     /old_locus_tag="pgaptmp_003310"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
     gene            1166..1960
                     /gene="hyxR"
                     /locus_tag="ID725_RS16555"
                     /old_locus_tag="pgaptmp_003311"
                     /pseudo
     CDS             1166..1960
                     /gene="hyxR"
                     /locus_tag="ID725_RS16555"
                     /old_locus_tag="pgaptmp_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            2114..2875
                     /gene="hyxA"
                     /locus_tag="ID725_RS16560"
                     /old_locus_tag="pgaptmp_003312"
     CDS             2114..2875
                     /gene="hyxA"
                     /locus_tag="ID725_RS16560"
                     /old_locus_tag="pgaptmp_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HGV7851841.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            2917..3999
                     /locus_tag="ID725_RS16565"
                     /old_locus_tag="pgaptmp_003313"
                     /pseudo
     CDS             2917..3999
                     /locus_tag="ID725_RS16565"
                     /old_locus_tag="pgaptmp_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            3989..4177
                     /locus_tag="ID725_RS16570"
                     /old_locus_tag="pgaptmp_003314"
     CDS             3989..4177
                     /locus_tag="ID725_RS16570"
                     /old_locus_tag="pgaptmp_003314"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGV7851842.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
     gene            4359..5027
                     /gene="ykgH"
                     /locus_tag="ID725_RS16575"
                     /old_locus_tag="pgaptmp_003315"
                     /pseudo
     CDS             4359..5027
                     /gene="ykgH"
                     /locus_tag="ID725_RS16575"
                     /old_locus_tag="pgaptmp_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(5270..5965)
                     /gene="ykgG"
                     /locus_tag="ID725_RS16580"
                     /old_locus_tag="pgaptmp_003316"
     CDS             complement(5270..5965)
                     /gene="ykgG"
                     /locus_tag="ID725_RS16580"
                     /old_locus_tag="pgaptmp_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGV7851843.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(5958..7385)
                     /gene="ykgF"
                     /locus_tag="ID725_RS16585"
                     /old_locus_tag="pgaptmp_003317"
     CDS             complement(5958..7385)
                     /gene="ykgF"
                     /locus_tag="ID725_RS16585"
                     /old_locus_tag="pgaptmp_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGV7851844.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7396..8115)
                     /gene="ykgE"
                     /locus_tag="ID725_RS16590"
                     /old_locus_tag="pgaptmp_003318"
     CDS             complement(7396..8115)
                     /gene="ykgE"
                     /locus_tag="ID725_RS16590"
                     /old_locus_tag="pgaptmp_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGV7851845.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(8642..9496)
                     /gene="rclR"
                     /locus_tag="ID725_RS16595"
                     /old_locus_tag="pgaptmp_003319"
     CDS             complement(8642..9496)
                     /gene="rclR"
                     /locus_tag="ID725_RS16595"
                     /old_locus_tag="pgaptmp_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGV7851846.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            9722..11047
                     /gene="rclA"
                     /locus_tag="ID725_RS16600"
                     /old_locus_tag="pgaptmp_003320"
     CDS             9722..11047
                     /gene="rclA"
                     /locus_tag="ID725_RS16600"
                     /old_locus_tag="pgaptmp_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HGV7851847.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            11156..11392
                     /gene="rclB"
                     /locus_tag="ID725_RS16605"
                     /old_locus_tag="pgaptmp_003321"
     CDS             11156..11392
                     /gene="rclB"
                     /locus_tag="ID725_RS16605"
                     /old_locus_tag="pgaptmp_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGV7851848.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            11404..11997
                     /gene="rclC"
                     /locus_tag="ID725_RS16610"
                     /old_locus_tag="pgaptmp_003322"
     CDS             11404..11997
                     /gene="rclC"
                     /locus_tag="ID725_RS16610"
                     /old_locus_tag="pgaptmp_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGV7851849.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(12157..13026)
                     /locus_tag="ID725_RS16615"
                     /old_locus_tag="pgaptmp_003323"
     CDS             complement(12157..13026)
                     /locus_tag="ID725_RS16615"
                     /old_locus_tag="pgaptmp_003323"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGV7851850.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            13275..14132
                     /locus_tag="ID725_RS16620"
                     /old_locus_tag="pgaptmp_003324"
     CDS             13275..14132
                     /locus_tag="ID725_RS16620"
                     /old_locus_tag="pgaptmp_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7851851.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(14253..18506)
                     /gene="fdeC"
                     /locus_tag="ID725_RS16625"
                     /old_locus_tag="pgaptmp_003325"
     CDS             complement(14253..18506)
                     /gene="fdeC"
                     /locus_tag="ID725_RS16625"
                     /old_locus_tag="pgaptmp_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HGV7851852.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDKNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            19072..19632
                     /locus_tag="ID725_RS16630"
                     /old_locus_tag="pgaptmp_003326"
                     /pseudo
     CDS             19072..19632
                     /locus_tag="ID725_RS16630"
                     /old_locus_tag="pgaptmp_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            19840..20196
                     /locus_tag="ID725_RS16635"
                     /old_locus_tag="pgaptmp_003327"
     CDS             19840..20196
                     /locus_tag="ID725_RS16635"
                     /old_locus_tag="pgaptmp_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851853.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
     gene            complement(20484..21410)
                     /locus_tag="ID725_RS16640"
                     /old_locus_tag="pgaptmp_003328"
     CDS             complement(20484..21410)
                     /locus_tag="ID725_RS16640"
                     /old_locus_tag="pgaptmp_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7851854.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            21567..>22448
                     /locus_tag="ID725_RS16645"
                     /old_locus_tag="pgaptmp_003329"
     CDS             21567..>22448
                     /locus_tag="ID725_RS16645"
                     /old_locus_tag="pgaptmp_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGV7851855.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQA"
BASE COUNT         6098 a         5339 c         4921 g         6090 t
ORIGIN      
        1 catcactaaa acattagacg taacacacat cccccacgcc ctgtaattca tagcttttat
       61 atttattttt tcacatcaat gtagataaaa acacgcatta aaaatggctt gtagaaaagc
      121 agcacaataa atagttcata tttagtctat tgcgtagaac tactattcaa aaaatcttat
      181 taatgagata atgagattac atcgataacg ttctgattgc aggcatactt atctgggtgt
      241 gcctctcttt tttacaactg tacacggtgg cgtatgacga gaaaatatct cacacaggat
      301 gaagtctaca ggctgatgga tgcagcccag agcatgtcct ttcctgaaag aaatcgctgt
      361 ctaattatga tggctttcat tcacggtttt agggccagtg aacttcttga tttacgttta
      421 tccgatattg atgcatcagg aaaacaactt aatattcggc ggattaaaaa tggtttctcg
      481 acaacacatc ctcttcttcc tgatgaatat aatttgatca agttgtggct taagcagagg
      541 aagttaattg aaaacggagt tgaaggagac tggctttttc tctcccggaa acgccgccct
      601 atcagcagac aacatttttt tctatcattc gtgaggctgg aagacgtgca ggattagctg
      661 taaaagcaca tcctcatatg ttacgtcatg cctgtggttt tgctctggct gacaatggtg
      721 tcgatacccg actacttcag gattatctgg gtcatcgaaa tatccagcat actgtcagat
      781 acacagccag taatgctgct cgatttaaag gggtgtggaa aaaaaagcct cgttaaaaca
      841 gtaccttctg attcaaaatg tcagattcct acgttttcct caattcttcc ataaaaaaac
      901 agcccattca attcaataaa acacataaca tgttgatatt tctcgctaaa gtaaaataac
      961 cccatgattt gaaatgatta accgccaagg tcataccagt gattaacttt cactcatgtt
     1021 gttaaatgca accaaattaa aaacaataaa aatcaaacaa attagattta tattccaaga
     1081 aaattaaata ttcaggcaat taaacaaaac ctgacatttt gaatcaaaat gtcaggtttt
     1141 gtagctatgg cgtggacgag tctgaatgaa gaagcctctg gttattatca gtgcctgtca
     1201 gtttaccagg ctggcactgg agtctctcat cccagcggac aggtatatcg tgcgggtgta
     1261 cagcaacgtc actacagagg ttgaattcgt tttaaagacc tcctgtggct atctgttggc
     1321 tgatatccct tagcttcctt cgggggagtt agttcttctg gcttgtcttg tccgcttgcg
     1381 gagtgatgcc gggcaatggc aggtctgcct gaccggtgac tccgactggt tcgataatgc
     1441 tcctgctgca agtctgtata acggcttccc acgcatcggc accatgctga cagcgagtcg
     1501 ttatcagaat caaattatcc gctggttact acgtcctttg cccggcgata tgagcgattt
     1561 ctggctcctg actgcacggg agttgagcgt gcttaagatt ctgatgcagg gcatgagttt
     1621 cagtgagatt gcccgttatg aacaacgcag ttcaaagacc ctgcatgcga ttgccacacg
     1681 ggcattaatg aaacttggac tgggaactct gagcgatttc aggctgctct atacgggatg
     1741 cggtgacagc agagtgaaac ggaatatacg gctacatgca agatatgtcg gacaacattc
     1801 cgctcatcga ctcactcagt atgtgcagaa catggtatcc ggaattcttt atccagagaa
     1861 agttgccgtt ggtgaatcaa taaattctga cgccatttcc ggaaaagtac ttcccggaca
     1921 aagaaaaaat atttctaaaa aaagaccagg attgttatga cacaacagcg ttgttactac
     1981 cccccactgc ggcgcgtacg gactcgctac agtgtagggg ccaaaatatt tctactgctc
     2041 tgggctgcag gattgggagg cacgctgtac agcctgtaca gcgtgctggc gtactgacat
     2101 ctgtgaggga aggatgcgtt tcgttctttt ctgtccctca ggaattgtac cagcgcagtt
     2161 cgcagctcta tcaaccggtt ctgtcggcaa tgttacctgc atcggaacag aagaggagtt
     2221 acgcaataaa ttgcggcaca ggccacagag tgtcgtgata tcggcagggc gacctgccga
     2281 atgcgctgaa atgtggttcc gattttatcg ggatcactca ttcgtggtgg tgttgtgcgt
     2341 cgcgccattt tttctgccac cggatgtgag tatttctggc gttctcaaaa acctgaggtt
     2401 actgaaacca ggaatgtcag tagagcatgt catcagtatt gcaaattcct ctggtgggtt
     2461 atcagggctg aaacatgcag aaaatttgcc agttatggac agttactctg tctttatgaa
     2521 agaggtgaat aaccgcacaa agacaatcgt catgtcagaa cgcttcccgg aaaagcaaaa
     2581 gaaggtgttg tcgttgcttc ttgccggaca cagctgggaa tacagtgcgc agtttctcaa
     2641 aacggggata agacagatat ggcttgccga gcagtcgcta aaaaagcgtt ggggaatacc
     2701 ggacagcatg tcattgcggg aagccctgct gctgagcagc aataaatttc acggtgatgg
     2761 aaatgcattg gaaaaaacga atgccatgac attgcgtgaa aacggaaata ccaacaggta
     2821 ttccgtggtg gtaaatgcag ggacgcaggt tttatctctg cataaatata aataagtggg
     2881 aaattgaaat tagttaccag aatggagaat ttcttcatga aaaacagtaa ggcattttac
     2941 cgcagcgcat tagcgacagc tattgttatg gctctttctg caccagcatt cgctgctgat
     3001 aacgcggtat caactgattc ggttacgctg aataaagata agacgactct ggatcaagat
     3061 gttgttatta gcaatacagc agacaaacag attacagccg taacaattaa tgcggcagat
     3121 gaagacctta atgttgcttt tgctggtcat gatattactg ctgaatcaac ggcagacaaa
     3181 aaattcgttg aaggtgtaaa agttagtggt aacaaaaatg ttgtgattaa tgctacagac
     3241 tccaccatca cagctcaagg tgaaggcacc tatgtccgga ctgcaatggt cattgattca
     3301 actggcgatg ttgttgttaa tggcggtaat ttcgttgcaa aaaatgaaaa aggtagtgcg
     3361 acagggatat ctctggaagg ggccaaggga aataatgtca cgcttaatgg cacaaccata
     3421 aatgctcaag gtaataagag ttccagcaac gcctccacgg caatttttgc tcaaaagggt
     3481 agcttattga atagttttga aggtgatgca accgacaaca ttacccttgc tggctcaaat
     3541 attattaatg gtcagattga aacaatagtt actgccggga ataagacggg aattcataca
     3601 gtcaacctga atattaagga tggctcagta attggggcgg ctaataataa acaaacaatt
     3661 tatgcctctg cttcggcaca aggcacaggt tcagcaacgc aaaatttaaa tttgtctgtt
     3721 gctgattcaa ccatctactc tgatgtcctg gccctttctg aaagcgagaa ttcagccgct
     3781 accacaacaa atgtaaacat gaacgttgcc cgctcttact gggaaggtaa tgcttatact
     3841 ttcaatagcg gcgataaagc gggtagtaat ctggatataa atctttccga tagttcagtc
     3901 tggaaaggca aagtttcagg ggcaggaaat gccagtgtat ctctgcaaaa cgagtctgtc
     3961 tggaatgtta cgggatcctc aactgttgat gctctggctc cgtctgggtg atgagtctgt
     4021 caatgccagc attgacggtg cagcattccg cgtgggtgca ggtgtacagg ctgatatcac
     4081 caaaaacatg ggagcatatg caagccttga ctacaccaaa ggtgacgaca ttgagaatcc
     4141 gctacagggt gtagttggta tcaatgtgac ctggtaagaa taaagcagtc ggttcccctg
     4201 tcgactgtta taccccctgc aggcagcagt atgctgcctg caggctataa atatccagta
     4261 ttcatgatgc tggctttttg ctgatattta acccgaacat tcctgatata gactattaat
     4321 tcatttatct gcggcagatt ttattttctg gatatattat gagtgaacaa atcaaacagg
     4381 atatcgatct gattgagatt ttattttatc tgaagaaaaa gattcttgtt atccttttta
     4441 ttatagctat ctgtatggca atggtgcttt tatttctgta tatcaataaa gataatataa
     4501 aagtgagtta cagcctaaaa ataaatcaga caacacccgg catacttgtt aactgtgata
     4561 gcaataataa ctttgcctgt cagacaacaa tgactgaaga tgttattcag cgaattacta
     4621 cattttttca caccagccca gatgtcaaga acagagaaat aaagctggaa tggtcaggag
     4681 ataagagaga tttaccaact gctgaagcgg aaatatctcg cgtgcaggcg tctattatca
     4741 aatggtatgc gtcagaatac cataatggca ggtaagttct cgatgagata caaacgcctt
     4801 cagcaattaa cagtgagctt tatacaaaaa tgatatacct gaccaggaac tggtcattgt
     4861 atccgaacgg tgatggctgt gtaactataa gctcaccaga aataaaaaat aaataccctg
     4921 ctgccatttg cctggctctg ggattttttc taagcattgt aatttctgta atgttttgtc
     4981 ttgtcaaaaa aatggtagat gaataccaac aaaactctgg gcagtaataa acagcattct
     5041 gatagagagt gcctgtataa agctgaagtg acaatgaccg ggaagggaac ccgttttcag
     5101 gaatggatgc ctgtcttttt cactgtaaat atcctggcat tagtaatgcc aggatattta
     5161 cagaagagga gcataaccat gacggaacgg ggttaagtca ggttgtcctt aacgctattt
     5221 tcaacctcgt atgccttctt caggtttatg tccagacttc atacctctct cagcaatcct
     5281 caataatcag atacaccgct ttcaccggac catgaacccc gacgactttg ataagctcaa
     5341 tgtccgccgt tgagctggga ccactgatga tgttaataca tgacggcatg cgttcaccgg
     5401 cctgcgcttt ctgatgcaat ctttctgcga gttgcgctac acgcggcagg atagtgcttt
     5461 tacgcaggat aaaaagagat gattccggga gcaggctcaa tgaacgccca cgctcggcgg
     5521 cggaaaaaag aaccacgcct cccgattcgg ttaaaccata ttcagcatac acgacaccca
     5581 ctttagcctg ctctgcctgc gagatattct tggcaccttt cgccggatcc caaacaacgg
     5641 cattgcattc ctgctgcaaa cgttcgctaa tccctaattc ctccagcctc gtgtcaccgc
     5701 taatcacgac cgactgatct cccagctctt tacacagacg tattgcagct tctgccgcct
     5761 tcgcctcgct ggtcagctca cagcgcgtca acataacatc gctggcaaac tgaataaacg
     5821 cgtcacagcg ctgctgttgg ttaagttggg taagccgctc gttagcatag ttattaagcg
     5881 gcgcatcttc tgcttgcggt tcaagtcgca gcgggcgacc cagtgcctga gcaacgttat
     5941 tcaaaaattc gcctcgatta tccattcttt ttctcctgcg cctgatgttt cttaaaccaa
     6001 ctacggaaac tctctccgtc agcttcagga agatcgcgtg cttccatcca gtcgctaatc
     6061 gcgccaaatt tgagtggtgt tttgccgcca ttgataaacc agcttgccgc atgagcaccg
     6121 gccatcatcc cgactttcca caatcctgga tgactattgg cataagcgaa catttttatc
     6181 gcccgttgct ctgcttttgc ggtgatccct ttttcagcca tcacccgacg atgacgcaaa
     6241 atcagttttg acagcggaat acgcaccgga cacacgttgt cacaagctgt gcataaagag
     6301 caggcgtagg gtaaatcttt aaaatcttta tagccgccaa gtagcggaga aatcaccgca
     6361 ccaattggcc ctggataaat agagccatat ccatgaccgc caatatggcg atatgccgga
     6421 caagtattca tacaagcccc gcagcgaata cagcgcagca catcccgaaa ttcagaggcc
     6481 agcacctcag aacgcccgtt atcgacaata accagatgaa actcttcagg accatcaacg
     6541 tgccccgctt cgcgaggtcc tgtcagccag gtgttgtatc ccgtcaaacg tgcaccaacg
     6601 gcactgcgcg ccagcatggt gatcaataca tctacctcgg caaacgtggg ggcaatacgc
     6661 tccattccca tcactgcaat atgcgtttta ggcagcgtgg tacacattcg cgcattacct
     6721 tcattggtca ccaggcatac cgaacctgtc tctgccaccg cgaaattaca gcccgtaata
     6781 cctatttcag cactgaggaa atcttcgcgg attttttgcc ggatgaataa ggtcatcgct
     6841 tcaggcgttt ccggcccctc atagcccaga cgttcgtgta gcactcgacg gatctgatgg
     6901 cgatctttat gaattgccgg gaccacaaca tgagagggcg gatcttgatc cagctggaga
     6961 atatattcac ccaaatcggt ttcaatcacc tgaatgccag catcctgcaa cacatgattg
     7021 acaccaatct cttcggtcac catcgattta gatttcacca ccttccgggc atttttgcgt
     7081 tgggcaacct gtaaaatgta gcgggtagcg tcttctttgg tttttgcaaa atagacgtga
     7141 ccgccgtttt gcgtcacttt ttctgagagc tggtacagat aagcgtcgag attactcaga
     7201 acatgatcac gtatctgggc ggcccgatcg cgccactcct cccagagccc caattcatcg
     7261 accatttttt gccgatttgc cccaatacgc tgctgcgcgt ttgccaccgc tttgcgcatg
     7321 atcggatctt caatttgctg acggatgcgt gtcttaaaat ctgtattact ggttttgatc
     7381 gacatcttta tatcctcagc ggctcatcaa cacttcagca atatgcatca ctttgacttt
     7441 ctgcccttcc cgttgtaatc gcccactgat gttcagcagg caactcacgt cagcaccaat
     7501 taaatactca gggcggactt ccatcaggtg cgcaaccttt tctttcacca tctcgccgga
     7561 tatttcggcc attttgaccg agaacgtgcc gccaaatccg cagcaggtat cctgttcagc
     7621 aaaggtcaac agctccagtc cacgcacatt tttcagcagc gtaagtggct cgtccttcac
     7681 ccccagctta cgggccaggc tacaagatgg gtgatacacc gctctccctt gcaaactggc
     7741 acctacatcg actaccccta atttattaac aataaaagag gtgagatcct gcatacgcgc
     7801 ggcaaccttt tcggctcgtg atgcccattc aggttcatcc gccagatacg tcgggtaact
     7861 ttttacggca taggtgcaag agccagccgg tgaaataatg ggatcgtcgt tatcctccag
     7921 tgcggcgatc agatttttca tccctggaat cgcttctttg atataaccac tattgatcgc
     7981 aggctgaccg cagcatccct gtttctccgg gaaatttacg cgacagccga gtttttccag
     8041 tagcagcacg gagtctcgtg ccattcttga tttcagggcg tcaccaatac aggtgacaaa
     8101 gaaattgaca ttcacaacta atactccatt acttcatgcc catttattcc cgatgtttcc
     8161 ccccacccgg ggatcaatca tcaataaata tggcgttctt ttttaattgg ctagcaacat
     8221 accacccctt atttgtatga caaatgatag tctttcatcc aaacttcaaa tgcaataact
     8281 aattttattt ctttcggcat cgctcacatt ttatggggaa atcgacgatc atttttggca
     8341 taagaaatcc atttaaaaat ggctacatcc cttataccta ttcagagcta cttattcagg
     8401 ggttattgag atatacactg atggtataca aaaaaattac cgacaataat cacgtatcaa
     8461 gtaatagtcg ggattttatt taattgaagc aatttagttt gcatttttat ttaaaaactg
     8521 agctagagtc catcagaaaa aaatatataa agcccaaacc accgttggaa atagaaatag
     8581 ttgaaaggct cacaactccc ggaatgcgga ttatctttgc cctgatttct ggcggtttta
     8641 ttcagggtgc aagctgtctg accctttccc gatattctcc cggagtacaa ccaaactcgc
     8701 ggacaaacgc cttgtgaaaa gatgattcac tggcatagcc tactgactca gcgatcacca
     8761 caacagggag catttcccgg gaaaacatct gggccgctat ttgtagacgc aactttgtta
     8821 atacagccag cggcgtggtt ccggaaacat cacggaaaag ctgggcaaaa cttgcccggg
     8881 acatgtgggc gatgctggcc agcgattcga cgctccaggc gtgtcctggc atttccagca
     8941 tttgctgtat taccgcacca agacgtggat gcagaagcaa actgagaatg tttttctcag
     9001 tattaacctg tgcaatccac tcacgcaccg cgagggtaaa gaatgtagcg cagatctggc
     9061 tacacagtgc atccactccc ggcattgccg atctggattc ctgttgtaaa aacgggatcg
     9121 cctccctcag ccagttatat tccacgctgt ggttaaccgg tgctaaaaac agcgtttccg
     9181 gcaaagacgt taaaaaataa cgtgccgaat gttgcagccg aagagtacca cagacaatac
     9241 aggtcgattc attatcgaca tgactcaggc gatgagctga attttgtggt agcaggacca
     9301 catttcccgg gcgtaatgta aaaatctctc ccgtcggcat ttccagcttc gccgctcctt
     9361 gcgttaacgc atgccaacga ataactgata attccccggc accatgtgga agctgccagt
     9421 cacttcctaa cacgcaattc ttatcgatcg ttccttgtgg agcgttaagc atcaacaaac
     9481 ggctaagggc atccatatca gactcctgag tggtttttga ttacaaacac caacaatctt
     9541 agtaaacaac taatctaata ccatcaaata gttaatcatg atttttgttt tgtgattctg
     9601 attaagaaaa agaaaatacc agacgataag acaaaatatc aagcgttgtg aagaaatgtt
     9661 atttgctctt ttgcgtctat agtcatgatg tcaaatgaac gcgtttcgac aggaaatcat
     9721 catgaataaa tatcaggcag tgattattgg ttttggcaag gctggaaaaa cattagccgt
     9781 cacgctggca aaagcaggtt ggcgagtggc tctcatcgaa caatcaaatg caatgtatgg
     9841 cgggacctgt attaatatcg gctgcatccc aaccaaaaca ttggttcatg acgcacagca
     9901 gcacacagat tttgtccgag ccatacagcg taaaaatgaa gtggttaatt ttttacgtaa
     9961 taagaatttt cataatcttg cagatatgcc caatatcgac gtgatcaacg gccaggcgga
    10021 gtttatcaat aatcatagcc tgcgtgttca tcggcctgag ggaaatctgg aaattcatgg
    10081 cgagaaaatt tttattaata ccggtgcaca aaccgtggtt ccgccaattc ctggaattac
    10141 caccacgcca ggagtatatg acagcaccgg attacttaat ctaaaagaat tgcctgggca
    10201 tttaggtatt ttgggcggcg gatatattgg cgttgagttc gcctctatgt tcgctaattt
    10261 tggcagcaag gtaaccattt tagaagcagc ttcgctgttt ttgcctcggg aagaacggga
    10321 tattgctgat aatatcgcga cgattttacg cgatcagggc gtcgatatta tcctcaatgc
    10381 ccatgtggag cgaatcagtc accatgaaaa tcaagtgcaa gtgcatagcg agcacgccca
    10441 actggcggtg gatgcactgt taatagcttc cggtcgtcaa ccggctaccg cttcgttaca
    10501 tccagaaaat gccggtatcg cagtaaacga gcgcggggca attgtcgttg acaagcgatt
    10561 acataccacc gcagacaata tttgggcgat gggagatgtt accggcgggc tgcaatttac
    10621 ttacatatca ctggatgatt accgcattgt acgtgatgag ttactgggtg aaggcaaacg
    10681 tagtactgat gatcggaaaa atgtgcctta ttccgtattt atgacaccgc ccctgtccag
    10741 ggttggtatg acagaagaac aagccagaga gagtggtgct gatattcagg tggtgacatt
    10801 gcctgtagct gcaattccgc gcgccagagt gatgaacgat acccgtgggg tattaaaagc
    10861 gattgttgat aataaaaccc aacgtatatt aggggcatca ctgctgtgtg ttgactccca
    10921 cgagatgatt aatatagtga aaatggtgat ggatgccggg ctgccttata gcatattacg
    10981 cgatcagata tttactcatc cgtcgatgag cgaatcactc aatgatctat tttcattagt
    11041 caaataaact caaaatcaga cgccagaaca aatattctgg cgtctcagag aaaagaatct
    11101 tattaattcc ctgttactct ataacttctt aatcacttca ttgatggtat tttatatgtt
    11161 taaaaaatcc gttttatttg caacactatt atctggcgtt atggcatttt ccaccaatgc
    11221 agatgataaa ataattctga aacatatcag cgtctcgtca gtatcagcat caccgacagt
    11281 tctggaggat gccattgctg atatagccag aaaatataat gcttcatcct ggaaagtcac
    11341 atcgatgcga attgataata attcaaccgc aacagcagta ttgtataaat aaggatattc
    11401 acaatggaaa aatacctgca cctgttaagt cggggagata aaattggcct ggcattgatt
    11461 cgtctgagta ttgcgattgt ttttatgtgg attgggttat taaagtttgt cccttacgag
    11521 gcagacagca ttacaccatt cgtcgcaaac agtccactaa tgtcgttctt ttatgaacac
    11581 ccggaagact ataaacagta tctgactcac gaaggcgaat acaaaccaga agcaagggca
    11641 tggcaatcgg ccaataatac ctatggtttt tccaacggtc ttggcgtcgt ggaggtgatt
    11701 attgctctgc tggttttggc taatcctgtc aatcgctggt taggtttatt gggagggctg
    11761 atggcattta ccacaccgct ggtaacactc tcatttttaa tcaccacccc ggaggcatgg
    11821 gtacccgcat tgggtgacgc tcatcatggt ttcccttatt tatccggtgc tggtcgtctg
    11881 gtattgaaag atactctgat gctggcaggt gctgtcatga taatggcaga ttcggcgcgg
    11941 gaaattctta aacaaggcag taatgaatcc agttcaacgt taaaaactga atattgataa
    12001 tccgaacact gtctgttgtt cactttttca ggcgtcaggc cccctttctg atgatgaagc
    12061 ctgacgccat attaccggag atatgacaga gtgaagatca atatcactcc cctgttaaaa
    12121 gattcttgtc gtctcaggta aaatacatga ttaatatcaa tcatgtattt ttacagcaag
    12181 acaaccacga acaaattcag gattaaaatg ttttaccgac tccccaacat aacccaaatc
    12241 aagcgaacta atttgtgcca tttctttctc tgtcagtgag aaatcccaga tagcaaaatt
    12301 ttcttcgata cgctgttgat gtgtcgattt cggaataacg accactcccc gttgaatatt
    12361 ccaacgcaga atgacttgag aaatcgattt ttgatgggca tctgcaatac tctgaagcag
    12421 attattttca aagggtttat gtcgtccacc acctaacgga gcccatgctt caggctgcac
    12481 gttataatac ttcatggttg ccagcgcctc tggttgggca aaatagggat gcaactcgac
    12541 ctggttgacc atcggtttaa ctcttaccgt ttcacaaaag ttcgccagaa catgaggata
    12601 gaaattggaa accccaatcg cttttaattt gccctcttca taggcatctt cgagtgcacg
    12661 ccacgcactg aaataatcgc gcatagcctg atgcaataaa tagaggtcga agtattctag
    12721 tcccgatttt tttaatgatg cttcaatacc tgctgctgcc gtatcctgat tcaacatatc
    12781 ctgcacccac aatttagacg ttataaataa ctcttctcgg gtacataaac cttcggatat
    12841 agcttcacga acggcttcac cgacggcatc ttcattaccg tataccgctg cagtgtcgat
    12901 gagtcgatac cccgtgcgga tagcgttcaa cactgactgt ttgcacacgt ttttatcggt
    12961 aacctgaaaa acaccaaatc ccatcatcgg cattttcaga ttgttactta atacggaaaa
    13021 ttccactgtc agtccctcca ttaggcatga acaatgagtc tacgttaaaa cgtaacctca
    13081 aattagtatg tggattttga tatcacttat gcaaaaaatt cattaataat gtaggactga
    13141 aacctctcta ttttcgggga caacgaagca gacgctacca gtgcttttgc cttcgccctt
    13201 gctgtttttg atacacttag ggcccagggc ataacgaaaa tgtgcgatat gacaaagaat
    13261 taacggagaa tgtgatgatc aggcagaaga ttctacagca gctcctggag tggattgagt
    13321 gcaatcttga gcaccctatt tcaatcgaag atatcgcaca gaaatctggc tacagcagac
    13381 gcaacatcca gcttctgttc cgaaatttca tgcatgtgcc tttgggagaa tacattcgca
    13441 aacgaaggct ttgtcgtgcc gccattcttg tccggctcac cgcgaaatct atgcttgata
    13501 ttgcaatctc tttgcatttt gattcacagc agtcattcag ccgcgaattt aaaaagttat
    13561 tcggctgctc tccccgtgta taccgccacc gtgattattg ggatctcgca aatatcttcc
    13621 cttctttttt aatacgtcaa cagcaaaaga cggagtgtag attagtcaac tttcctgaga
    13681 cacctatttt tggcaattca tttaaatatg atattgaagt gtcgaataaa tcaccggatg
    13741 aagaagtcaa actgcgacgt catcatttag ccagatgtat gaagaatttt aagacggata
    13801 tctatttcgt ctccacgttt gaaccgtcaa caaaatcggt cgatttactc acagttgaaa
    13861 cttttgccgg tacggtatgc aaacaaaccg attcagagat gccgaaagag tggacaatta
    13921 accgaggact gtatgcctct ttccgctatg aaggtgagtg ggagcactac cctgaatggg
    13981 cgcgaaacct ctatctgatg gaattacccg ccagggggtt agccagagtg aacggcagtg
    14041 atattgagcg gttttattac aatgaagatt tcgtagaaaa ggatagcaat gatgttgttt
    14101 gcgaaatatt tatccctgtt cgtccggttt agctgtcgct atatctaaat gaacttataa
    14161 cgagactact gtcaggcaat tcaataaata atggcgttga agatgtcaac gccattattg
    14221 tttattagaa tgttacttcc atattcttaa tattagttca tcgcctcctc gccctttgcc
    14281 gtatcggcat ccaccgcggt aatggtcacc gtcactggct tcatttcgta cccgccatac
    14341 gtcagggcgt taaatgtgaa cgtatagaca ccaggcttgc tggtactaaa tgtacgcgtg
    14401 atttttccgc tcacgaaatg gtcatttttc gacggcagga actgataatc cttctccgtc
    14461 accccttctg gcgtcttaat gtcgacccac atattccccc caacggggtt atttgccgac
    14521 atcagtgtca cctccagctt cgcagtcgat atcccatccg ccaccagaga agtcacatca
    14581 gatgatgcgg tgagtactgc atttttatca tccgcgacga atttagattc ggcagttttc
    14641 gtcgattcct gaccgtcggc ctgactcact ttcgccgtga gtgtatattt cgctgcgaca
    14701 gtggtcgtgg cggtgaaaat agcctgtcct tgctcattag ttttcgccgt cccattggga
    14761 gtcagaacta aatttgccgg gctggcagtc agcgtcactt cgctatcttt caacaggttg
    14821 ttattggcat cagtcaccgt aactttatac gtctgtttgg cattgccgtc cgccagtgca
    14881 tagttgccaa tcacctcaat accggagacg gttgcggtag ccttatcggc tacaaacgtt
    14941 atagtctgcg tattcgaagg ctggctattc cctgccgatg ccgtgatctc atacgttccg
    15001 gcagtcactg aaaccacctg caactctgct ttcccctcat cgttggcttt cacccagaca
    15061 ttatcgcctg taagcggctt gacaccccgg ggcagattaa aggtcaccag gctccctgga
    15121 acagggttac catttttatc ctcaacgttc gccgtcagcg taaaggcatc tttgccgttt
    15181 gccacttttt gagcagcggc gtctacctgc aggtttgcct gaccggtgct ggcatccgcg
    15241 ttgaatttca ccgtgaccgt cttctgagca ccattcaccg aagcggaaat attgtgttct
    15301 cccgcaaccg ttgacataag ctcaatgtcc gctttacctg ccgcattagt tgttactgta
    15361 ttccccgtct tgctggtcac accctgcggt aaagtcagcg taacttcctg cccctgcaac
    15421 gggttaccat aggtgtccac aacggtcagg gttatctggt tagcagactg tccattagcc
    15481 agttggttat taaccttcac tgtcatatca cgaatctcag ccttagatgc gtcacctgca
    15541 acgttcagca ccagtggctg agcaacggca ttttggccgt tcactcgcgg catcacagac
    15601 aactgacccg cggcagatcc cagcgttaag gtcgacacgt agaccccatt acctttctct
    15661 gtccagtttc ctgctgaagg acgctcactc cccgtgctgg cggcaccgct aaacactggt
    15721 gcatctggct tcagcccggt gaccgggttc ccgtacgcat ccttcacggt gaaggtgagt
    15781 tccgtcgtcg ttttgacggt cggagcctta ttgtccgcaa caagcgtaga gttcgctgat
    15841 gacatctctc cggcgatgac cgtcaactgc gcggcttcgg tggctgctgg ctggccgttg
    15901 aagagaggca tgacgcgaag ctcgcccgtc tttccacctg tagtcaacgt agcaacatag
    15961 gaaccgttac ctttttcggt ccaactggaa acggtcgccc cttcagaggc ggtccccgtc
    16021 aaacttgccg acaacgcaag accactgata gcgttgccat gcgcatcttt cgccaccagc
    16081 gtcacggttg tgctttcgcc ggcttttacg tgatcttctg cgacagagac tttcgactgg
    16141 tttgaagata acgcatcagc caccacggtt acttttgccg catttgctgc cgcattctgt
    16201 ccatttagct tcggcataac ttctaattca cccgccgtag agccgagagt aatctgcgca
    16261 gtccacgtcc catcaccatt atttgtccag cccgatgccg tagaaccttc agcagcggca
    16321 cccgctaatg acggcgcttc cggcgtgagg ctggtcacag ggttgtcata ggcatctttt
    16381 accgtccaga ttgccgtaac tgtcccccca accaccggtt tatcaggatt cagggtgatg
    16441 gacgaatgtg ctgcatcaag cggcccggca acaaacttca gcgtttgttg taatgtgccc
    16501 agctgatact gctcgctgaa agcacgcaca acaacgtttc cggcacgggt cgaagaaacc
    16561 gcggcgctgt aaacgcctgg ttttatttcc gaaatggcac caacggttac accattagtg
    16621 tcttgcggaa caaatcgcaa gcggctggct tctcccgtca ccggattacc ctcggagtcc
    16681 accgcagtca acgtcaacgt ataggcttgc tgaccatcag caaccacgtc acctgacggc
    16741 tcgttagcgc tcagggtgga gtttgccaca tccatcatcg tggcccgcag ttctgcagtg
    16801 acggttttgc tcatgccatc aacgctaaca gtaatcgttg cctcacctga ctgcgttccg
    16861 gtagtaaaga cagactgata cacccctgct tcagtttcgg tgaactcacc cagtgttggc
    16921 tttgcctgtg atttagtggc cttcagggaa cgagtcacaa tatttccagc cggtttgaaa
    16981 gttagttcag tcttgatctg atctttcatg cccgtgactg gctggccctc ggcgtcgcgc
    17041 agagacagca ccagcggttt ttgctcatta ccgttagcaa gcatttgaat acggctctga
    17101 ccgtcaagcg ttaacgccgt gcgatcggcg ctcataccag ctccggtaat gaccacctct
    17161 gtctgcacgc gttttgaggt attgcctttg ttatcgtagg caactgctga aatcgcataa
    17221 taattgtctt tgcctggacg ataagccggg agcgttactt gccactgact accctgaccg
    17281 gtaattttgc caccttcagc cagtaatgac ggcgcttccc actgcacatt tttcagtccg
    17341 tgagttgctt tgctgaccac aagccccagg gaaagtgtct gaccgccctt accttcaata
    17401 cgctcaggca gagcaatacg gatcacttca gatttgcggt actcaagaac gatgttgtta
    17461 ttacgctcaa ccaggtcata gcggctgcct gccagtaccc gacgctcgcg aatgctatcc
    17521 gtatcgagtt gtttcgccaa aggttcgcca attcggtagt taacttccag gccaaagcga
    17581 gtgtcattct caccgctctt gccctgctta tgcccggcgc tcagtgtcag aagaggcact
    17641 ggcgtatagg tcacctcggc agaaatagca tgcgggtctt tctggcgctt atctttacca
    17701 aacagcccga cttcatcgcc ataatactgt tcatacatca ggcttgcgcc aagctgcggc
    17761 caggcaggta aatagccctc tgcgcggata tcccaaccat tcgccgggcg ttcctgataa
    17821 tcctcaatat ccggcgattt tttccagcca gaagcccgaa tataaccatt ggcgctcagt
    17881 ttcagataat cgcgccagta ttccgcacca acaccaatgc gggtatgact acgggataaa
    17941 tcatggtcga taaaggtgtt cacccccgcc atccagtcat ttcctgaaaa atgacgccag
    18001 ccaaaaccaa tatttgactg agtacgatcg tctgtacgat gtattgcccc ctgagtgaac
    18061 aacatatttg tcggcgtatc ataaatcgga taaagcattt ccagcgaaga atccttcagc
    18121 gagaaatctt tatcgacatt cagtttgacg cgcgctgtac catatttccc gagccactcc
    18181 tgtatttcct ggttagcttt agcggtggcc attccggtaa taaaattacg tgtcgcatcg
    18241 ctatctggct gactgcttaa aaatgtcccg gcatttgcgg caaacgacgc gacatttttc
    18301 tccacgttat tatcagcagt taccgtagta tttcccatgc tcaaccgtgg ctgaaccgca
    18361 tgctgcgcac gtgccgccat tactggggta aaggtgacag cgagtggaaa aagaacctga
    18421 acagagatat ttgcccacgc cacgcagcgg gccagaactg aataacgaaa tcgtggttgt
    18481 ttatgacctg ttttataacg tgacattgag ataatttcct gatgaacgaa ttaacgaaca
    18541 taggcagaca caaataatgg cgtctgcttg tcagaaaaac cggggatttc ccggtttcct
    18601 tttccgcaga aaaggaaata cctttatgta agaaagtaca ccgccaccta atatatccag
    18661 tgcctgtagc cactcatcag gtggttgggc gcgggattat atttttattt atgggtgaaa
    18721 atttagcttt ttgtgcaagt cattaaatag atacttaagt tatatttctt actctgcgga
    18781 cagtgaataa ttcattattc agagtgaata ataatgacct gcctttaatt agcaactatt
    18841 ggaaatacgt cgaagtaggt atatacgcca ggataaaccg atatattttt tatggatatt
    18901 cattttcctt catcggactg taaattgcgg agatatcgaa cttgcactac ccatcgcaac
    18961 actcaaacag atttatgaat ttcgttcata acccctgaca aatcctgccg tctaatcgag
    19021 ctcttctctt tacgttatgc ttctcatgaa cgataacgga aggataacac catgcaaaaa
    19081 gtaaaactga ataacggtat tgaaatgccc ctgctgggct ttggtgtatt tcagatgacc
    19141 gatgccgccg aatgcgaaag agcggtcatt gacgctatta acaccggtta tcgactgatt
    19201 gacaccgccg cctcctatca aaatgaaccc caggtcggga acgcactgaa acaaagcggt
    19261 atcgcccgta acgaactctt tgtcaccacg aaactctggt tgcaggatac cagttacgaa
    19321 ggggccaaag cccagttcga acgctctctg aatcgactgc aacttgatta cgttgacctg
    19381 tatttgattc atcagcctta cggtgatgtc cacggtgcct ggcgtgccat ggaagagctg
    19441 caacaggccg gtaaaattcg cgctattggc gtcagtaact tccatcctga ccgactggct
    19501 gaccttatcg cctttaataa cgtcgttcca gcggtaaacc aggttgaagt taaccctttc
    19561 aatcagcagc tacacgccgt tccgtggatg caaagccgtg gtattcagcc agaagcctgg
    19621 gcaccgtttg cagttacagg gcggtaagcg attatgcatc tacctattta cgtcggtcac
    19681 tcagtcagag tcttgcccat attcaatgag gttcaacacc gcagccaatg aaaagtaggc
    19741 catgcgggga ttaatagcat tactcattaa ccaatcgctc ttttcattta cgtgcttttt
    19801 atgtaacata ttgcttttat tgatcttcct ggagtttgca tgaataagaa tctcatcctc
    19861 gcatttgcat tattttcctt acctgtattt gcagaagaag atctggggcc aggcaaatat
    19921 gtttgtgaca ttaggatttc cagcctggat accgctactc aaattctgtc aaaatccgca
    19981 acagttctcg ataatggaaa caatttcatc gttcaaatgc caaatggaga tcaattgtac
    20041 tctccagatc tggaaaatgt tgatgatggt ataaaacaaa aagcaacaat aggtggagtg
    20101 acatttattc gtcgtccaac ttttaacgat cgcttcatcg tggaagatgg aaatacagga
    20161 ttcttctata agatgcgaaa ctgcgagaag aaataaagga agtttgatag cgatatcctc
    20221 ctgatttcag gctttgtacc tgttcaagac ttacaactac aagccgctac gtgttaacgc
    20281 ctcccaaaat aaccaccgtt aaccgtagcg gtcatagata ctattatttt aaccacatta
    20341 ctggtgataa tgataattga tattatggcc gtatagaaaa taagtattaa ttatcagaaa
    20401 ctttattatg taaacgcatc ctaatgatta cgttattcct cttaccgata ttcgcccaga
    20461 gcaaccatca attacacata tcatcaattt tcgagtttta ctcttttctc caccataaaa
    20521 tcaataaaag cacgaacttt agagggcatt ctcacccgtg atgaaacata aatataaaac
    20581 ccaggaaatg aaacagccca atcctcaaga atcggctgta acttgcccgc attgatatgt
    20641 tcagcgattg caaaatccag atgttgaatg atacccgttc cattcagcgc cattcgtatc
    20701 atgctttcgt catcattaga aatatactta cctgaaacct ctatttcgaa ctcttcatta
    20761 ccccccacaa ccttagaaaa ctcccatgca gagagtgcac cgctactttt atgccgcaac
    20821 cgaaggcaat tatggttact cagttcctga ggcgtttcag gaataccgtg ttcttttaaa
    20881 taatctggcg atgccacagt aaccagttta ataagcggag aaacaggaac ggcagtcata
    20941 tgctcatcca gtccttgctc aagacgtatg cccacatcac agccgtccgc aataatgtta
    21001 gataaaccgt catccataat gagttctatc tttgttttgg ggtaacgagt aagaaactcg
    21061 ccaatatgag gctcaacaag catccgtgct gcaacacggg aggaatttat tctcaacagg
    21121 cctgtaggtt caagttgggt atcaccaaca tctttcaaag catcatcaat ttgccccaaa
    21181 gccgacacca gcacttcata cagacgctgt ccttcctcag taagcgagat attgcgggtc
    21241 gtacggtata tcagcttggt gttaagacgt tcctcaagag attttaaatt ctgggacaat
    21301 gcggccagcg taatacccat ttcagaagcg gctctggaaa aactgccggc acgaacaatc
    21361 agcgcaaacc aggcaagaga aggcaaaatt gaaggctcta ttttcatcat tgtaaacctt
    21421 aacttaacac tgtgatagat attagaccat tgatgataac caatacaagt catactatta
    21481 cgccgttctc gatatgaaca caaatgccag ttgcggataa tagaaatgat atttaaatct
    21541 gtttaggcaa atccccggag cttgaaatga aaactgtttc gataaaacac acttactggg
    21601 atatagctgc tgatatctat ttccctgacg attttaatag tgaaaaaaag tatcctgcca
    21661 ttatcagcgc ccatccaatt ggaagctgca aagaacagac atcgggtagt gtgtatggtg
    21721 ccgccctggc aaaagctggc tttatcgtca ttgcttttga tgctagtttt cagggaagca
    21781 gcggtgggga accgcgttat ctggaagatc caacaatgcg agtaaaagac ttcagcattg
    21841 tcgttgatta ccttaccagc ttaccttacg ttgatgccgg gcgtattggt gtactgggta
    21901 tttgtggtgg aggtggatat gccattaatg ccgcaatgac agaacgccga attaaagcta
    21961 ttggtactgt cacgggtgca aactacgggc gtcttatgcg tgagggattt acagcgttta
    22021 atcctatcgg agcacttgaa gcaatggcgc aacaaagaac agacgaagct aacggcgcaa
    22081 aacttcgcgt agatgattta ctcccctctt ctccggatgc agctcttgaa gcgggactaa
    22141 cagaaataga cctttttgaa gccacagaat attatcgttc gccacgaggc tatgcaccta
    22201 atggggtaaa tcgttcgcta ttctcacatc agactgttgc cgtgggttgg gatgcatttc
    22261 atcttgcaga agtgctgtta actcaaccaa taatggttgt ggtgggcgat agagtgggcg
    22321 catttggtgc ctaccgtgac ggctgcgaaa ttattggccg cgctgcttca aaacataaag
    22381 agctggtcgt ggttgaaggt tattcacatt acgacctgta tgataagcca gaacctgtga
    22441 aacaggca
//
LOCUS       DABSYT020000074        22017 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.074, whole genome shotgun sequence.
ACCESSION   DABSYT020000074 DABSYT020000000
VERSION     DABSYT020000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.074"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(44..196)
                     /locus_tag="ID725_RS16650"
                     /old_locus_tag="pgaptmp_003330"
                     /pseudo
     CDS             complement(44..196)
                     /locus_tag="ID725_RS16650"
                     /old_locus_tag="pgaptmp_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(193..313)
                     /locus_tag="ID725_RS16655"
                     /old_locus_tag="pgaptmp_003331"
                     /pseudo
     CDS             complement(193..313)
                     /locus_tag="ID725_RS16655"
                     /old_locus_tag="pgaptmp_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(353..616)
                     /locus_tag="ID725_RS16660"
                     /old_locus_tag="pgaptmp_003332"
                     /pseudo
     CDS             complement(353..616)
                     /locus_tag="ID725_RS16660"
                     /old_locus_tag="pgaptmp_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            710..1059
                     /locus_tag="ID725_RS16665"
                     /old_locus_tag="pgaptmp_003333"
                     /pseudo
     CDS             710..1059
                     /locus_tag="ID725_RS16665"
                     /old_locus_tag="pgaptmp_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1717..2148)
                     /locus_tag="ID725_RS16670"
                     /old_locus_tag="pgaptmp_003334"
     CDS             complement(1717..2148)
                     /locus_tag="ID725_RS16670"
                     /old_locus_tag="pgaptmp_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HGV7851856.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(2216..4237)
                     /locus_tag="ID725_RS16675"
                     /old_locus_tag="pgaptmp_003335"
                     /pseudo
     CDS             complement(2216..4237)
                     /locus_tag="ID725_RS16675"
                     /old_locus_tag="pgaptmp_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4371..4496)
                     /locus_tag="ID725_RS16680"
                     /old_locus_tag="pgaptmp_003336"
     CDS             complement(4371..4496)
                     /locus_tag="ID725_RS16680"
                     /old_locus_tag="pgaptmp_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HGV7851857.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(4438..4587)
                     /locus_tag="ID725_RS16685"
                     /old_locus_tag="pgaptmp_003337"
                     /pseudo
     CDS             complement(4438..4587)
                     /locus_tag="ID725_RS16685"
                     /old_locus_tag="pgaptmp_003337"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(4761..5522)
                     /locus_tag="ID725_RS16690"
                     /old_locus_tag="pgaptmp_003338"
     CDS             complement(4761..5522)
                     /locus_tag="ID725_RS16690"
                     /old_locus_tag="pgaptmp_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGV7851858.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            complement(5519..5953)
                     /gene="psiB"
                     /locus_tag="ID725_RS16695"
                     /old_locus_tag="pgaptmp_003339"
     CDS             complement(5519..5953)
                     /gene="psiB"
                     /locus_tag="ID725_RS16695"
                     /old_locus_tag="pgaptmp_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGV7851859.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(6008..7966)
                     /locus_tag="ID725_RS16700"
                     /old_locus_tag="pgaptmp_003340"
     CDS             complement(6008..7966)
                     /locus_tag="ID725_RS16700"
                     /old_locus_tag="pgaptmp_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGV7851860.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(8032..8265)
                     /locus_tag="ID725_RS16705"
                     /old_locus_tag="pgaptmp_003341"
     CDS             complement(8032..8265)
                     /locus_tag="ID725_RS16705"
                     /old_locus_tag="pgaptmp_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGV7851861.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            complement(8322..8774)
                     /locus_tag="ID725_RS16710"
                     /old_locus_tag="pgaptmp_003342"
     CDS             complement(8322..8774)
                     /locus_tag="ID725_RS16710"
                     /old_locus_tag="pgaptmp_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGV7851862.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            complement(8800..9006)
                     /locus_tag="ID725_RS16715"
                     /old_locus_tag="pgaptmp_003343"
     CDS             complement(8800..9006)
                     /locus_tag="ID725_RS16715"
                     /old_locus_tag="pgaptmp_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGV7851863.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            9076..9525
                     /locus_tag="ID725_RS16720"
                     /old_locus_tag="pgaptmp_003344"
     CDS             9076..9525
                     /locus_tag="ID725_RS16720"
                     /old_locus_tag="pgaptmp_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851864.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSSSSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVRGGA"
     gene            complement(9611..10174)
                     /locus_tag="ID725_RS16725"
                     /old_locus_tag="pgaptmp_003345"
     CDS             complement(9611..10174)
                     /locus_tag="ID725_RS16725"
                     /old_locus_tag="pgaptmp_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGV7851865.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(10221..11582)
                     /locus_tag="ID725_RS16730"
                     /old_locus_tag="pgaptmp_003346"
     CDS             complement(10221..11582)
                     /locus_tag="ID725_RS16730"
                     /old_locus_tag="pgaptmp_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HGV7851866.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(11634..11864)
                     /locus_tag="ID725_RS16735"
                     /old_locus_tag="pgaptmp_003347"
     CDS             complement(11634..11864)
                     /locus_tag="ID725_RS16735"
                     /old_locus_tag="pgaptmp_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851867.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            12381..12890
                     /locus_tag="ID725_RS16740"
                     /old_locus_tag="pgaptmp_003348"
     CDS             12381..12890
                     /locus_tag="ID725_RS16740"
                     /old_locus_tag="pgaptmp_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851868.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            complement(12860..13051)
                     /locus_tag="ID725_RS16745"
                     /old_locus_tag="pgaptmp_003349"
     CDS             complement(12860..13051)
                     /locus_tag="ID725_RS16745"
                     /old_locus_tag="pgaptmp_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851869.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(13048..13470)
                     /locus_tag="ID725_RS16750"
                     /old_locus_tag="pgaptmp_003350"
     CDS             complement(13048..13470)
                     /locus_tag="ID725_RS16750"
                     /old_locus_tag="pgaptmp_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGV7851870.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(13517..13942)
                     /locus_tag="ID725_RS16755"
                     /old_locus_tag="pgaptmp_003351"
     CDS             complement(13517..13942)
                     /locus_tag="ID725_RS16755"
                     /old_locus_tag="pgaptmp_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGV7851871.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(14360..15136)
                     /locus_tag="ID725_RS16760"
                     /old_locus_tag="pgaptmp_003352"
     CDS             complement(14360..15136)
                     /locus_tag="ID725_RS16760"
                     /old_locus_tag="pgaptmp_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851872.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(15182..15736)
                     /locus_tag="ID725_RS16765"
                     /old_locus_tag="pgaptmp_003353"
     CDS             complement(15182..15736)
                     /locus_tag="ID725_RS16765"
                     /old_locus_tag="pgaptmp_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGV7851873.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(15630..15851)
                     /locus_tag="ID725_RS16770"
                     /old_locus_tag="pgaptmp_003354"
     CDS             complement(15630..15851)
                     /locus_tag="ID725_RS16770"
                     /old_locus_tag="pgaptmp_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851874.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(15852..16535)
                     /locus_tag="ID725_RS16775"
                     /old_locus_tag="pgaptmp_003355"
     CDS             complement(15852..16535)
                     /locus_tag="ID725_RS16775"
                     /old_locus_tag="pgaptmp_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGV7851875.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(16612..16917)
                     /locus_tag="ID725_RS16780"
                     /old_locus_tag="pgaptmp_003356"
     CDS             complement(16612..16917)
                     /locus_tag="ID725_RS16780"
                     /old_locus_tag="pgaptmp_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851876.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(16921..17823)
                     /locus_tag="ID725_RS16785"
                     /old_locus_tag="pgaptmp_003357"
     CDS             complement(16921..17823)
                     /locus_tag="ID725_RS16785"
                     /old_locus_tag="pgaptmp_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGV7851877.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(18518..19489)
                     /locus_tag="ID725_RS16790"
                     /old_locus_tag="pgaptmp_003358"
     CDS             complement(18518..19489)
                     /locus_tag="ID725_RS16790"
                     /old_locus_tag="pgaptmp_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HGV7851878.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(19489..20655)
                     /gene="sopA"
                     /locus_tag="ID725_RS16795"
                     /old_locus_tag="pgaptmp_003359"
     CDS             complement(19489..20655)
                     /gene="sopA"
                     /locus_tag="ID725_RS16795"
                     /old_locus_tag="pgaptmp_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HGV7851879.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(21243..21998)
                     /gene="repE"
                     /locus_tag="ID725_RS16800"
                     /old_locus_tag="pgaptmp_003360"
     CDS             complement(21243..21998)
                     /gene="repE"
                     /locus_tag="ID725_RS16800"
                     /old_locus_tag="pgaptmp_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HGV7851880.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVVKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
BASE COUNT         5046 a         6369 c         5537 g         5065 t
ORIGIN      
        1 ttgatgttgt tatataaatt aatgtattcg gtggtgcatc caattagtag aacatgtgtt
       61 tttcgataaa cgctccgatc actttttcgt ggatctcaac tgagcgtgag aagcagatgg
      121 ttttacgagt caaccgctta atgcgggtgc gtagcgtcag attattacgc tcaatgcgtt
      181 gggtgaatat tttgccagca cgttatcacc gaacatctct cccccagtcg gcgaaccctt
      241 tattggacgt cagcacaatg ctcgcttttt catatcgacg gttcagcaac cggaagaaca
      301 gactgtgttc ctgcgtaaag cgctgctccg gtgtcttcct gaactggcga agcaacatgg
      361 aacggccacc ggtataaccc atcgctttga tttccgccag gataacttcg ccgttccaga
      421 cattctcagc cagacgcatg tcgatatagt ccatttcggc cattttgtgg cgtgtttttc
      481 tggccggagg ttctgggtat ttcaggtaac gtctgaccgt tcgttcagag caaccaatct
      541 gagtcgcaat atcaacaata tacgcacctt gctggcgcat ttgctttatc atgtaaaagt
      601 cctctctgct cagcatgctg atgtcctttc tggtgtgaga acctcaagga aacaacatgt
      661 tgggtggagc ggacaatcca aatggtgaat taccgtctta tatcactggc ggataaggat
      721 aagtacacgt cggcagttcc gtcagcactg cgacagcgtt gtcctcgttg cttttacccg
      781 gtcatgcaca acgcctgatg gatgaactgc gtgcccaggg ttaccatttt aaagtaaaaa
      841 ccgtgacgga aagcctgcgc cgtcacggac tgagggcaaa agcttcctgg aatttcagtc
      901 cggtctgtta ccgcgcacac agccagcctg tgtcagaaaa tctgctggag caggattttt
      961 acgccagtgg cccgaaccag aagtgggcgg gagacatcac atatttacgt acggatgaag
     1021 gctggccgta tctggctgtg gtcacgtgcc attattggct gatcaatgtc gtcacgcatg
     1081 acggcacagg agcccccgga acgttatgct accgcatgtc atttgcaaaa atgacagata
     1141 gatatacttc aatttgaatc ttaacaatta gcatgggaat gttgtcaatc ctctgtatgg
     1201 agccaccaca tattgcaatt tttgttctct tttaaaatat attccacgag cgtcttgtgg
     1261 gaccttttgc tgttataggg gggcagtttg gatatcgaaa attattgtac gttaaggctg
     1321 aatttcaatc gcaaagcata accttacctt tctgcttact tatgcagttc aaagagtacc
     1381 aactctgcaa ttttcgttgc cacagatgca atatcgttac tttccagtca ccatgccgct
     1441 gaaagcccac accatgcgat aatccacatc aagagccgct ttctgtccag acccgctgtt
     1501 gtgaggtgtg aggtgtgagg tgtgaggtgt gaggtgtgag gtgtgaggtg tgaggtagcc
     1561 tgagtttaac ggacaggccg atgaaggacg ccgttaccgg gataatgtaa gaatcttttt
     1621 ttgagtatgt caacataacc tgaggcaaag tacctcaggt tccagtaatc agccgacctg
     1681 acagtcttct gttagcccta cagatatgtc gtgtagctaa ccgcgcatgt caaagccgga
     1741 tttttgaaaa tctctataaa cttccggatt gttgaaggcc gggaatttag ctttatgggc
     1801 tgctgacttt atcgcttcgc aataggcttt atcgccatta ctggttgata tttttaacgc
     1861 cgtgccatcc cgggagaatt ccatatgcaa cctgcatttt ttccttttcc atttttgcgg
     1921 atcatcaagt ttagcattaa ttgcatccct gattccccgt gcctgctccc cccattcatc
     1981 ctgatcatcc caacgtcctg aactgcaact acccagggct gtagttttat ggcaatttga
     2041 aggatgtaat ggcgcacatc ccgccacaaa gctgactaaa atagccagta taacgatttt
     2101 ctttaccccc atatattgct cctcaaccca tatagatctc agtaccatag ctctgtcaga
     2161 gcaaagaaaa tcttaactaa tacaagttaa gatttttaat atccactcct catccctaca
     2221 ttccaggtga tggtggcgtc cactcattat cctgaactgg agcataagat gcgccatgcc
     2281 ccccggatat gcttttctgt tgcctgatat attcgtggta gtactgttca ctacgctgac
     2341 gatgtaaatc cacatccggg cggctgatta caaagtgtcc atttccggtt attccagctg
     2401 cattatgcca ggccaccatt tccgcaaata catcattaaa ctgttgatat gagccttccg
     2461 gttcaaaacc cattcctggc tcacgatcaa acaatacagg agcaatcaga ttcagcttat
     2521 ccaccagttc atttctgtcc tgatgatggt agaacaccac acggtcccca taatccacac
     2581 agactaactc gccattcctc cgatcacgaa aacgcacact gccatctttc tgcagacggg
     2641 cttcgagaac gccagtgtct tcataaagtg gagttcctcc cggttcacag agaatcacgc
     2701 agcggtcggc attcgttgtt cgtcgcttgt ctttgcgacg atcacgataa tcccagccac
     2761 gtaactgtga taatgcagct ctgtctccct gtaccgcctg ttgctccacc cactgccggt
     2821 aggacaaagg ataccactta ccctcctcaa tcagtgacaa ccgctcttcc ttcacactct
     2881 ccttcagcct gatcagcgcc tgcatacgct gcacttccgc aatatggtaa tgcagcttac
     2941 gcaattgtgg atcccggaac tgcacacgga tatacgcctt gcgccgacgg caggcagcat
     3001 gaatttcccg taaccgttct ccgtaacgta agtcgggttt acgccagtgc tccttccatg
     3061 caagataacg cgcgcgcaaa tcttcccgcg cagctgcacg ggcttcacgg cgttcacgac
     3121 gcagtacagg atcgcgccgg aagccgggct ccacttcatc gctggcagca agttccggat
     3181 tgtagcgaca ttcaggcttc acccgttcaa atatatctgc acctgcaatc tcaaatggcc
     3241 ctgcctgcgg ctcagccctg gataacgtca aatccgggtg gatactgctg gccttcactg
     3301 gtgtgagctc atgattaaac gcatccacaa tcacaagacc atgatgctgt ttctgcaaca
     3361 taagcccttt acgcgcaaac atctcatggc acgcctgcca gctgaaattc ccgcccgcga
     3421 tcatatcttc aatcaacgac tcacactgaa cagttgtgta cccccacaga ctttccttat
     3481 cagaaaacag ttcgcgcttt attgctccct gtggacctgt ccccatccgc atacgatacc
     3541 attcgtaatc actgacagcc tgagtaccaa gattacgtcc ccggaaccgt tctccgggta
     3601 taacctgagt aaaaatatcc ttcggtacag gctgccatcc accatccaga tcctgcatat
     3661 aatccagcgt cattaacggc cacacactgt ctgcacgtac tggtgtctgt tgtttatcaa
     3721 atccatcatg cagtaccaga tgcccgtgct ggatccccat ccataatcca gccgttgcca
     3781 gagttctgtg cacatccagg caactttcca gctcaccatt tctcgccatt tccggtaagc
     3841 gttcggcaaa atgacggcag gcgtactgtt tcagactctc cgtttctgcc actttttccg
     3901 gccggatatc aacgtttatg gcttccggat cataacgacc gggtgtcccc acctgactga
     3961 aaatatctgt cggtacaggc tgatattccc ccagtttccg ccccagtttt tcagctgtcc
     4021 ataacggtcc gaacgaactg agtgcaacac cttcccgcgc tctgtcccag ccatccttca
     4081 ccaccagttc cccttcctgc atcgtgatat acagaccatc actggccagc cgtttatgca
     4141 aagataacca gtcctgtgcg ggctcttcac gcagcccggc aatcgacatc tgagcaatat
     4201 attccctgaa cgtctgcttt ttcccgcgtc gccaggctgt tcatgcccgt acaatgccag
     4261 caaggagaaa ggggccaccg gcgaaccagc agccccttta taaaggcgct tcagtgcagt
     4321 cagaccagca tcagccctga aaaggcgggc ctgcgcccgc ctccaggttg ctacttaccg
     4381 gattcgtaag ccatgaaagc cgccacctcc ctgtatccgt ctctgtaacg aatctcgcac
     4441 agcgattttc gtgtcagata agtgaatatc aacagtgtga gacacactat caacacacac
     4501 cagacaagag agctgcgtgg tagtttcatg gccttcttct ccttgcgcaa agcgcggtaa
     4561 gaggctatcc tgatgttgtc taagcatgca ggggcctcgt gggttaatga aaaattaact
     4621 acggggcttt tgtccttctg ccacacaaca cggtaacaaa ccaccttcac gtcatgaggc
     4681 agaaagcctc aagcgccggg cacatcatag cccatatacc agcacgctga ccacattcac
     4741 ttttcctgaa aataacccgc tcattcaggc tgtttacggg caatccgtgt gattgttgcc
     4801 gcatcacgca cctcccggag tttgttcggc accatccacc gctccatctc acggacagca
     4861 tgaccggcct cctttgctgc cagactggct tccgcgataa cctgccagaa tggcatatcc
     4921 tgccactgcc agcgttccat tccggccagt gacggaaaac gctcgcccca ctgccagaac
     4981 agggtttcca ggtcatgctc tgccacccga tcactccagc gtgcatacca gcttccgact
     5041 gtttccggtg aatggaacgc cagttcgatt tcagcctggg cgaccagcgc ctggtacgca
     5101 cgggctttct gctctgcctc cctcgcctgc tggcggctgt acttctgcat gaccagcgcg
     5161 gatttatggc gactgcgttc cctgacacaa aaccgcaccg acgggaacaa gcgttctgct
     5221 gcatcaccgg gaaggggaag gaggcagacc tcgccctgtg tttcaatcag cacatcgatg
     5281 gcatacaacc actgaagacg gtttccgtgc agttcttctg cggtcaggga cggcatgaaa
     5341 aatcgcaggt cagacagcga tatcctgcgg cttccgttca gacagcggaa aaacgccccc
     5401 gcatacggat attccgccag cgtgtttccc tgattgcggc gtttttccgt ctctgccact
     5461 gcccgccatg cggcctgttg ctctgtgctg aggggaacca gtgcctgtga acgtgcgctc
     5521 atacctgacc tccttccgcc atcaggatat ggatgatgtc gttaacgctg tatccctgcg
     5581 tatccagtga cgccgccagt gccagtgaat ggctgacggc ttccggtgca aaccgccgct
     5641 gtacctgaac caccgcaaac ggctcaccac cggcattcac cagaaccagc acccagacct
     5701 gggagacatc gcccggtgaa cataacgcca ggtgaaaacg ttcgtgggca ggcgtgaaac
     5761 ggacctggac ggggaaaaag ccgccaaact cgctgccgta ctcgccgttc atcgtccagt
     5821 tgtccggcgc atccagctcc cgcatcacgg cgtgtgtcag ctcacggcgt gcatcacttc
     5881 cggcagcccg gatatcttca tattcctgtg cgttcatggt atgtaataca ttcagagtca
     5941 gttcagtttt catcacggta tctcccttgc tgccgccccg tctccggcgg ggcggtgtgg
     6001 ttgttcatca ggcggcatca gccaggttat cggtgcgttc agtggcatcc gtctgtgggc
     6061 gtggtgcaca catccagcca ggcacccagc ggttgtcttt catatggtgc tctgcatgtt
     6121 cagccgcatc gcctttcttc atcttctccg cgtcccgtgc ggcaccggat agtcctgcct
     6181 cattcagggc tgcgataatc tgcggctttt tcaggtgtcc gaaaaagttt gctttcgtcg
     6241 gctgccacca gtcgcgcatg tgaaagccga tggcgctctc cagcgtgtcc agcggactgc
     6301 gtgacgtgtg accatactca cgggtctgga caccgtggat actgcacgcg gtgcagaaac
     6361 tgagcagtga taacagcacc tcctgactga gtgacaggaa cgtcgtcata tcccgtgccc
     6421 atccatccgg cagcagggcg gcaagacggg ctttttctgc catcatcgcc atgaatgcgg
     6481 caccttcctt ccctgacggc gcatcgctgg tcagcgaata atgctcacat tccaggcgga
     6541 ttctggcggg attgctgtac gctccactgc caaatacatt cagacagagt gtccatgcca
     6601 gaagtgccag ggatttgtct gattgctgca tcagtgccgc ctggactgcc agcgtgcgct
     6661 ctgaggacat tttcgtcagc agcggcagac tgatttcctc cactgaggct ttctcctgca
     6721 cctgttccgt gcggtaatcg ttatcagcca catcatcctc actgcgcaac tgcacaccac
     6781 gctggacaca cacgttgcca taacgccagg acaccaccac gccacttccg gcacgcatct
     6841 ccggcgtcca cgccctgacc ttcgccatgc agcgcatcag cttcatttcg gcttccagca
     6901 ggtcggattc ctcacactgg ttttccagcg catcgtaacg tgccatcagt tcgttcaggc
     6961 gttcttcttc tgcctccgtc agcaccgctt ccggctccgg cagactgcgg tatgttccgg
     7021 catcctcacg gcagaaacca acaggctcca tacgtccggc gcaccattcc cagccttcgg
     7081 cttcccgaag gtgttcggcg acagcctgga gtttttccag cagggcggca tcgagcgcca
     7141 cgcagtccac atagccaccc tcgtcgtcac tgaacaaatc ggtgcgcagt tcatctggcg
     7201 agaaggtatc agcccccacg aagcggaatt tactgttccc tgccaccgcc acttcacttt
     7261 cggtcaccag acgacgaatg gtctgtactt ccggtttacc gccccatccc gactgacagg
     7321 cggcttcaaa cacctgcacc tgacgcgcgg tgtcgttctc cagcgccagc gcctgacagt
     7381 gctcggtggt gatgcggtct tctgccagcg catcgaggat gacaggcgca aggtctgcca
     7441 gtttcagcat tcgctgaacg tggcggggcg aataacccag caaatcaccg atttgtgcag
     7501 gtgttttgcc ttcctgcgcc attgcacgga atccggcaat ctgttcggca gggtgcatat
     7561 cccgacgatg accgttctcg gtcatcgatg cggcagtcgc cagctcctgc ggaatgacct
     7621 tcacacggac aggccagtca gccggaatga tgtcacgctc tgccagcatg ttgagtgctg
     7681 ccagtcggcg accacctgcg gcgacaccgt aacggtcacc tggcagggca tgaacaacca
     7741 gattctgtaa caggccgaca cctttaatgg agtccgccag ttcgctgacg gactccgcag
     7801 aatacggcac cgtgcgcaca ttcagcggtg acttaatcag cgaggccagc ggcacgctca
     7861 cttcctccgt ctgcgccagc agggccgaca ggactgtttc ctgcgttttt gcaggtttac
     7921 ggctggattt acgctccgtt tttgccttag actctgtaac tgacatagtt agtacctccc
     7981 aaaacggtaa taatgattgt ctgccggacg cgttcccgtc cggcacctct cttacttaat
     8041 aatcccgaaa tcacggatga catggttcat ccagtacccg gcaccgtcct caaaattcca
     8101 ggtacgccag accattgcac cgttattacg gacaaccagg cgaaactgtt cgccgtggtc
     8161 attctcgata aagacgttcc ggtactgtgc ggcaacggct tcggcatgtt tgcgggtaaa
     8221 aggaccgtca ggcagtccct gaagtattct gaaatattcg ctcatcctca tctctccggt
     8281 gatgccccgc acaggacggg gcggttgtca cagtcagtcg ttcagaacgg gatatcgtct
     8341 gaaaacccgt aatcgtcacc ctccggcggt tgcggctgag gctgaccgct gaactgttgc
     8401 ccctcttccg gctgagtctg agcacctgcg gcacgtacca gcatctgcat ggtgcccgtg
     8461 gtcttaacaa gaatttcggt gacgtaacgg gtgataccgt tatcctccca gctacgggtg
     8521 cgaagctgac cttcgatgta gacctgcgcg cccttgcgca gatattcacc tgccacttcc
     8581 gcgagcttgc cgaacagcac cacgcgatgc cattctgtct gctcccgcat ctcccccgtc
     8641 tgtttgtcac gccagctttc tgacgtggcc acctgcaggt ttgccactgc gcccccgttg
     8701 gggatgtaac ggacttccgg atcttttccc aggcgtccta cgaggatgac cttgttaatg
     8761 ccacgaactg ccatgatgtg tctccttctg ttgatgtttt cagcccccat catcttttga
     8821 gtttcgggag ccgcggtttt gacgttttgc ggggacctgc cttttgttcc ctccggtatt
     8881 caccatcacc tttttgagaa ccttcgccgc aaggggcatc actgccacgg ccggaaacga
     8941 ctgtccgggg gcagaccgca aaactttttg cctgcttttg ggcaaaaagt tttgcgggat
     9001 gtgcatttca cccctggact ggcggagacg gttgtggctg tctttgcccc cggcgacagg
     9061 ggcttaaaca ggtgaatggc accggacaca acaacgggca ggcacccgcc gcaacgacgt
     9121 gaagcgcggt ttgcgaaacg gcgttgcccc ttacatcccg gcggcctttt gccgggatgc
     9181 cgggagcgca ggccacggcg cgacagaggc ggcgcgaccg gaacggtcgc tgcaagcccg
     9241 ggtttcgcgt cagggatgga agcccgccag ggcggagaca gctccgctgg ctccgttcac
     9301 gacagcccgc cccgccaggg ggacgcccgg aaaaggcata agcaggagaa caacaaaaca
     9361 gacagtaatc agagcagcag aaaaaagaag aacagtagca gtagcagtag cagtagcagt
     9421 agcagtagca tgaacaacaa catcatcagt aacagacaaa tgacaggtct gaaaagacag
     9481 aagcccacag cagaatgtcc gcgtaatgta aggggcggcg cgtgagcgcg gcccctttca
     9541 gcgccaccgg cgcgcggtct tttcccggcg cgggcagacg ggcatttatc ctcccgcctg
     9601 cccggagtca tcaggcacgc agacgaataa tcatcgtcgg aacatcggta taagcaaacg
     9661 tgccgcgtgg cagctcctcg cggacgtcag aaaacggtaa cagcttctcc tgctggcgcg
     9721 gcccgttcag acagacggcg accagcacgc cacccggacg caacagggaa aaggcccgca
     9781 ggatatgccg gatatcctgc ccgtggctga acggcggatt aatgataacc cggctgtaat
     9841 actgcaccgg ctgccattcc atgaagtcgc cacactgaac cctgacaccg ttaaaatttt
     9901 cccgcagata ccggaccagc ccgctgttga tttccaccgc atcacacatg gcctccggtg
     9961 cggtatcccg aatcgcccgc agtatggccc cggtgccggc agagggttcc agaatgtgat
    10021 cacggttgct gatctccgcc agcgtcacca ggcgatcaca gaccggggca ggagtcacaa
    10081 aaagctgaga ttcagcccgg atttcagcaa ccggcgcggc gttatccagc cttgcgcgca
    10141 tcagccggat acgttctttt aactgactgt gcatgtcatt atctccgtca tcccccgggg
    10201 atttcccggg gggatatacc ttatttctgt gggatttccg taattttcat gtcggtgata
    10261 tacacattca ccagacggaa attattgtcc attgtgcggc ggtagcggta tgccccgtgg
    10321 tcttctgctt ctgccacact gcgcacggcc ttacagtccc ggggcagggc ggcccactgt
    10381 gcctttgtca tttcccggaa accttccccc ggatagttga ctaccggcgg acgcttcgcg
    10441 gcctggctgg cgacggcagc ctcttctgcc gatggtgctt tgtagtccgt gatgcgatcg
    10501 ggcgtcactt tcatcgtgcc gctgtacccg agaaaactgt aattcggcgt tgtgactgaa
    10561 ctcaccgccc cgttgctttt gttcacgcgg atgatggtca gccactcgcc ccggctgaaa
    10621 acctgtccgc ccggctcaaa atcctgtgtc cgggtaacca cgccgccgct ttcgtccagc
    10681 atggcacgct catagttcag gcggttctga tagtggttaa cccagcgttg ctgatgctga
    10741 atctgccgtt catgacagcg gatcgcaatt tcgcgggcct gctccgtggt gatgatgtca
    10801 tcatccaggg ccgaccataa cgaccggctc ccttcatact ggcttttttc tgccgggcgc
    10861 ggatacgtat ccagcgggaa acaggcgctg atatggtcat gactgctgat gagttttgcc
    10921 atattcagat ccagcgattc agcccgccac atcgtcagat atttctgcga ctgcgcgatg
    10981 gtcttttcag ccttgcgcaa atcagcttcg atttttttga tacgacgcca gcgaacgtcc
    11041 ggacgttctt tatacttcgc gtgaagcagt gctgaccgcc cccgttcttc ccagtattcc
    11101 gcacgttcaa agagcatcac ggcacgtttc atgccgtttt caatacgctg cgcatcacga
    11161 cgggcgcggc gttcgctgtg atgccccaca agaatgggct gaccgggcgg gatcatcgcg
    11221 gccagtcttt ccacttcatc aagtgcctgt gcggattcac tggcccgctt tccgctgtat
    11281 ccggtaaacc gctccgcccg tgcttcctga cgttcagcga gcgtgctgtc ttcatcctca
    11341 atctctccgg caagtgagag gagcacgtct tcccggcccg gtgtccaggc cggcgcgaca
    11401 aacagcgcct gttttggtgc ccagcgaaaa ccggcatcat gcactttttt atacgtctcc
    11461 gggtcaagac gtgacacggc atacaggcgc agtttgttgt catccggtga gtaggtcgcg
    11521 cggtacatct gcacagggct ggcggcttcc ggggcggtca tggtgattgc tgctgatgtc
    11581 ataaaatgtt ctccttccgt ctgttgccgc ccctcgcggg gcggctcacc tcatcagtta
    11641 aaagcgcgtt tcagttcgag tgtgcggagc acctgcccga aagccgtgtt tttcatggcg
    11701 gggatgtgta caccacgccc ccggtgaaaa ctccgggcta tgtgtcgccc gaaggctttc
    11761 agcacggggt caatttccgg agcggggatg tcttcgcgtc tgaaacgggg atgacggcgg
    11821 ggctttacag ccgctgcgga aggtgaaaca atgcttacag tcataaatgg attccttatg
    11881 acaaattggt taacaatgcc gtcaaaattg gtcgcacaat tcttacggcg tggattaagc
    11941 aggtccctga cctgcttttt tcatatctgc atctgacgct ttccggtgtt ttcctccttc
    12001 tgttcctgcg ttctcattac ctgtcagccg gatgactgac agaacacata aatgcgtatt
    12061 gccggagcct gaaataaatc ctgattttta aagaacatca cccgttgagt ttcgggagcc
    12121 gcggttttga cgtttcgcgg gaacctgctg ctgtattccc tgtggtatga acttcacccg
    12181 tttgagaacc ttcgccgcaa ggggcatcac tgccacggcc tgacacacct gtcctgggca
    12241 gaacgcaaaa ttttttaccg ggttctgggt aaaaaatttt gtgggctgtt catttaccct
    12301 ggacagtcag tgcggcaggt tgtggctgta tcagctcccg gcgacaggga ctcatacggg
    12361 tgtgtggcac ccggcaggca atgagcaggg acccgccgca acgacgtgaa gcgcggtttg
    12421 cgaaacggcg ttgcccctta catcccggcg gccttttgcc gggatgccgg gagcgcaggc
    12481 cacggcgcga cagaggctgc gcgaccggaa cggtcgctgc aagccagggt ttcgcgtcag
    12541 ggatggaagc ccgccagggc ggagacagct tcgctggctc cgttcacgac agcccgcccc
    12601 gccaggggga cgcccggaaa agccataagc aggagaggaa cggaaagaag aggaggaaga
    12661 ggaagaggaa aaagaagaag aacagtaaca ggcacggtaa ccacagaaaa ataacatcag
    12721 aaaaaaacat tccggacaca tcacaaacag gaagggtaac ggacgacgcg tcagcgtggc
    12781 ccgcttccct tccggcaggc gtcagcctgc caggggtttt cgcgcgggct gacaggcggc
    12841 ctccaccgcc agccctgtat cacctgtttc tgctgccctg taagatgtag tgaccgccca
    12901 ggaaaacagc ctgctgattc gccacaaaat cgcagatttc atcaacaatc tgcgcggtgg
    12961 tgagcggttt ctgccccatt tcacgacgtc gcgcattaat cacggctttg taattgcgca
    13021 gaatttcgcg ggtttctgtg ctgatattca tgacatgtgc tccccgtcca gcgcctgtaa
    13081 cacaacgtcc attgcttcgc gttcctccct gacgaacgca tcaccgtcgc ccccgttctc
    13141 actcccgacg gcatacaggc gcttcatttc actgcctgcc agcgccatca ctttttccag
    13201 catcaccgcc ggaactgtca cctgacggct ggcacgcttt tcttccatca actcacgaac
    13261 aacgtcgtga acaatgctga catccgcacc atattcaaag gcatcgtcca gccgctgcat
    13321 gacggtttcc agctcatcgt ctgacaggtt ccagtcctgg gctatgtggc gcacatcccc
    13381 acgggtcagc accacggcaa aaacgcattc actgtccggc acgtctgtat ccagtacatc
    13441 gcggatgatt tctttaacag tacagtacat gttctcgtct cctttacagg ggcggcattg
    13501 ccgccccgtc cgttaatcag tccagtgccg cgtaaatcgc gttgcattca gggtgaaatt
    13561 cgatgtgacg ccacaactgc gcatcgcgca tccggtaaag ctgggtcagt cctgcatcac
    13621 cgctgtcgtg gtacagccac aactggcggt taatcacaag ggaagtcaga atgatgccgc
    13681 aggcatcagc actcacggta cggtcaaacc agtttgcacc gttcaccatg tggaaacggt
    13741 cgccatccgg catcatgtaa ccgccaccgt ccggcaggcg gatgaagtgc cagaagcctc
    13801 cctgatattc cggcatcatt ttttgcgcca gcgcgtacac atacgcctcg gcatacagaa
    13861 aatcgttgcc aaacaggtaa ggcagaaagg agagacggtc gcactcttca acattcagag
    13921 tgacaggttt cgcatattgc atgatgtttt cctccgttgt tcacatattc cgcgacaccg
    13981 ggcggtgccg tgcctccaga aaagccgggc gataaggcgg ggatgcacag ggcgcaaggg
    14041 aagccgcagc ctgacgcaca cggctgacgg gagttgcagg gtgaggccgc gaaacctgag
    14101 cgggcgaacg accggaaaat cacggcagac gggaaggagg gaccgggtga ccgcagcggc
    14161 gaagcgtacc cttgcatcaa cgccagcccg gctaagatgg aggcacggca ccgcccgcac
    14221 acgacgctga ggccacataa ctgaaaaacc acaaggccat gccctgcatg gccgggtaaa
    14281 gcggagccag cggcgacttc ctttgtgctc tgtccgggga ccggcaggaa aaaggcgggg
    14341 atttaccccg ccaacgtcat catttccgga tttttgccag ggcatccgcc atgctgcgga
    14401 tttcgttccg ggcttcccgg accacctccc gcagaacacc acggtcaaac caccggcggg
    14461 gcggttcatc cggtatacgc agcacatcgc aggccagccc actggacagg atatgttcaa
    14521 tacaatcgct gaccagatac atttccaccg ccgaaaaacg tgcgccccgg ttcaggatgg
    14581 catcgagcac cacgggcagg ttttcgtcgt cctcatcgag ccagcagggg tgatcgcttt
    14641 ccttcacgac caggcgaaag cggcgcgtct ggcgggtcag taacgtctga ccggtggtgg
    14701 tcagcgccgc ccggatacgc cgcgtatctt ctgccacccg gcaactgaca ctgcgtaccg
    14761 catcgcgcac cgccgcaagt acagccctga tttcgtcttc ggtcagccat tcaccggcat
    14821 acggaaaaga cacctgcgtt ccgtctgccg gaagcaccgg cagcgactgc cactgactgc
    14881 cggacagaac aagttccgct ccggttccgg tcagtttctg aatggcggag gccaggtgcg
    14941 gcggcagtac gccgccgtgg cggttaacga gtaacgtcgc cagccggggc gcatccagca
    15001 gaacataaat gctgatccag cccggatact tcgggttacg ctggtcgaca tccgtctctt
    15061 ccggccagcc ctgcgccagc agcaggttac gggcgtgacg acaaatcagt gcgttcagcg
    15121 ttgtgttttc gttcatgatt aatcgttctc cctgtatcgc cgcccgcagg cggcgataac
    15181 atcagccgtt ttcgtgtgtg ttgtttttca gcaggatgcg ggcctgatta accaccgcct
    15241 gacggcgggt aacgcattcc gggacggcga gaccgtaatc acgggcggcc cattcacgtt
    15301 tccacagtgc ctgttcggcg gtctgaatca gggacgccag cagttcagcc gggacggtca
    15361 cctggcggtt ttcgctcacg ttgctgataa tctccatcgc cgcggcagaa gaaataccgg
    15421 actcaattcg ctggtcttcc gggatgtcct ccaggcgcgc caggacggtc ctgatttcag
    15481 gatcggacag ggaaatatcc attccgtcag cgagggcctg aatctcttcc ggggaccaga
    15541 taaccagcat cagcggtgta tatcccggat actttgctgc cagctcccgg catagcgttt
    15601 cgcatgttcc gtacattctc gtctccttct caatctttaa agcacgcatc cagttccaga
    15661 atttcggtat acgcctccat gaacaggcga ttggcgcgat acagcgtctg gcgtgccagg
    15721 cgttcaacaa gcacatcgga atcaaagcga cagcgatgct ccatatcgtt cagcctggcc
    15781 cgaaggtcac acatggtgtt ggccacctcc gccacgtcgg cgcgaagttt ttcatgtccg
    15841 gcatagttca tttacgcagc ctccggcata aaccagtcat catttgcggc cccctgctgc
    15901 atggcccgct gtacggcagc cagacgttgc tgtccggcac ggtgatactg ttcaagcagc
    15961 tcgataccga tataccggcg tccggactga agggcggcca cacaggttga gccgctgcct
    16021 gcaaacgggt ccagcacaat cgcgttcggg tgtgtgaagc tctcaatcag tggttgcagg
    16081 ctggtaacag gcttttccgt cgggtgatga cgattgcctg aatatttcca gcccagcacg
    16141 tcgggcaatg gcttctgagg cagcgccgga cgcccttttg ccaggatgta agcgcattcg
    16201 tggcgatagc ccacatatgc ggccttcgaa gtgtaggttt tggtaaaaac caggtggcca
    16261 accacgctga atcccgcatt tttccaggcg gccataaagc gatcgacgcg gttccagcca
    16321 tagaagctca ccatcagcgc atcttttttc agcacgcggt acatttcatt acaggccggt
    16381 tgcagccatt catcggtttt atcgccggcg atggtacgcc cctgacggtc acggaaaccg
    16441 acgagatatg gcgggtcggt gaggatgaaa tcgacaccat tgcccggaat cgtggccata
    16501 acacgcacgc agtcaccctg gataaaacgg gacataacag cactccttct cttacagagg
    16561 gcaccgggag ggcgcccctg tggcgcccgt tccccgcggc ctgtgggctg ttcaccgcgt
    16621 ccggtaacgg gtcaggccga ccgtggaata tcgcagccgt cgtctttttg acggcgagaa
    16681 tatgccgcga aagcggcttg aagggcgttc atcagaaaag ggcgacgggc tggcgtacac
    16741 tgtcaccgat gccagccggg agcggtaaaa gcgcccgtcc cgtgcccggc cggacgcggg
    16801 acactgacca caggcagcgt tcctgccgcg ccgcgtcagc cggcgcagta aaaagaggag
    16861 cccggcgaca tcctttaccg gaaaagaaac gggcggttgc ccgcccgtct ccgtcatact
    16921 tcatccaccg taatgcgcca ccttgtcgac tatccactcc ggcgcggtga cttccggcag
    16981 ctcatcatca tctgtcgggg aagaccattc cagttccccc cacagcacat caacaagctc
    17041 tccacgctca tatcgctgcc agccacagaa attgcagccc tgcccggcgt accagtgctc
    17101 cagcatgcaa ctgaaacggc ggcttaatac tgcaataacg tcgctttccg gctgacacca
    17161 cggcgtgtcg aaatcaacct gaatgaagtt gtctccctga ctgctgatat tcacctcata
    17221 cccacaaggc cacttcacgc catactgttc cgtataccag tgatatgcag aaggaacacc
    17281 gttcagaaca ccaccgttaa agccattcac ttcaacatcc aggcgagtgg acagaaccgt
    17341 cagcaggtca aacggcatgg ttttttccgg caggacgttg tcacacagac ggttccacca
    17401 cacagataca tcctcgttac tccagatgtc gcaccagtca cctctttttg gggtgaaaag
    17461 agcggtaatg ctttccctgg catcatccgg taattcttcc cagcgacgcc ggccagtacc
    17521 gctctgcagc cagagttcat gcagcatcag gcagttttgc tcatccagaa gtacaccgtc
    17581 ctgcaggtgt gtcagccagc gggtgaacgc gatattctcc ggcgatacaa cgccacgccc
    17641 ggcagccgtc agtccggggc atggttcaaa ccgcacatct tcagtggtct gcagaagtcc
    17701 ggcacttccg gccagaaaca gctgaatacc ttcatttgtg gcgcggcgat aaagcggtgt
    17761 gactgcaccg ctggccagtc gtttaatctc agcgatctgt gccggttcac cagaaaaata
    17821 catacgattc gagcaccagt ttggcatggg aattctcctc tcacatgcgg cctccatcag
    17881 gccgtgccgc acctgtaacc gggcgaccgc atacgaatgc aggtgcggcc ccgaagccgc
    17941 agcgcgtgga ggaggaacga cgaaacgtga ccgggtgagg aggccggcga cagcgacaca
    18001 cttgcatcgg atgcagcccg gttaacgtgc cggcacggcc tgggtaacca ggtattttgt
    18061 ccacataacc gtgcgcaaaa tgttgtggat aagcaggaca cagcagcaat ccacagcagg
    18121 catacaaccg cacaccgagg ttactccgtt ctacaggtta cgacgacatg tcaatacttg
    18181 cccttgacag gcattgatag aatcgtagtc tcacgctgat agtctgatcg acaatacaag
    18241 tgggaccgtg gtcccagacc gataatcaga ccgacaacac gagtgggacc gtggtcccag
    18301 accgataatc agaccgacaa cacgagtggg accgtggtcc cagactaata atcagaccga
    18361 cgatacgagt gggaccgtgg tcccagtctg attatcagac cgacgataca agtggaacag
    18421 tgggcccaga gagaatattc aggccagtta tgctttctgg cctgtaacaa aggacattaa
    18481 gtaaagacag ataaacgtag actaaaacgt ggtcgcatca gggtgctggc ttttcaagtt
    18541 ccttaagaat ggcctcaatt ttctctatac actcagttgg aacacgagac ctgtccaggt
    18601 taagcaccat tttatcgccc ttatacaata ctgtcgctcc aggagcaaac tgatgtcgtg
    18661 agcttaaact agttcttgat gcagatgacg ttttaagcac agaagttaaa agagtgataa
    18721 cttcttcagc ttcaaatatc accccagctt ttttctgctc atgaaggtta gatgcctgct
    18781 gcttaagtaa ttcctcttta tctgtaaagg ctttttgaag tgcatcacct gaccgggcag
    18841 atagttcacc ggggtgagaa aaaagagcaa caactgattt aggcaatttg gctgtgttga
    18901 tacagcgggt aataatctta cgtgaaatat tttccgcatc agccagcgca gaaatatttc
    18961 cagcaaattc attctgcaat cggcttgcat aacgctgacc acgttcataa gcacttgttg
    19021 ggcgataatc gttacccaat ctggataatg cagccatctg ctcatcatcc agctcgccaa
    19081 ccagaacacg ataatcactt tcggtaagtg cagcagcttt acgacggcga ctcccatcgg
    19141 caatttctat gacaccagat actcttcgac cgaacgccgg tgtctgttga ccagtcagta
    19201 gaaaagaagg gatgagatca tccagtgcgt cctcagtaag cagctcctgg tcacgttcat
    19261 tacctgacca tacccgagag gtcttctcaa cactatcacc ccggagcact tcaagagtaa
    19321 acttcacatc ccgaccacat acaggcaaag taatggcatt accgcgagcc attactccta
    19381 cgcgcgcaat taacgaatcc accatcgggg cagctggtgt cgataacgaa gtatcttcaa
    19441 ccggttgagt attgagcgta tgttttggaa taacaggcgc acgcttcatt atctaatctc
    19501 ccagcgtggt ttaatcagac gatcgaaaat ttcattgcag acaggttccc aaatagaaag
    19561 agcatttctc caggcaccag ttgaagagcg ttgatcaatg gcctgttcaa aaacagttct
    19621 catccggatc tgacctttac caacttcatc cgtttcacgt acaacatttt ttagaaccat
    19681 gcttccccag gcatcccgaa tttgctcctc catccacggg gactgagagc cattactatt
    19741 gctgtatttg gtaagcaaaa tacgtacatc aggctcgaac cctttaagat caacgttctt
    19801 gagcagatca cgaagcatat cgaaaaactg cagtgcggag gtgtagtcaa acaactcagc
    19861 aggagtggga acaatcagca catcagcagc acatacgaca ttaatcgtgc cgatacccag
    19921 gttaggcgcg ctgtcaataa ctatgacatc atagtcatga gcaacagttt caatggccag
    19981 tcggagcatc aggtgtggat cggtgggcag tttaccttca tcaaatttgc ccattaactc
    20041 agtttcaata cggtgcagag ccagacagga aggaataatg tcaagccccg gccagcaagt
    20101 gggctttatt gcataagtga catcgtcctt ttccccaaga tagaaaggca ggagagtgtc
    20161 ttctgcatga atatgaagat ctggtaccca tccgtgatac attgaggctg ttccctgggg
    20221 gtcgttacct tccacgagca aaacacgtag ccccttcaga gccagatcct gagcaagatg
    20281 aacagaaact gaggttttgt aaacgccacc tttatgggca gcaaccccga tcaccggtgg
    20341 aaatacgtct tcagcacgtc gcaatcgcgt accaaacaca tcacgcatat gattaatttg
    20401 ttcaattgta taaccaacac gttgctcaac ccgtcctcga atttccatat ccgggtgcgg
    20461 tagtcgccct gctttctcgg catctctgat agcctgagaa gaaaccccaa ctaaatccgc
    20521 tgcttcacct attctccagc gccgggttat tttcctcgct tccgggctgt catcattaaa
    20581 ctgtgcaatg gcgatagcct tcgtcatttc atgaccagcg tttatgcact ggttaagtgt
    20641 ttccatgagt ttcattctga acatccttta atcattgctt tgcatttttt tattaaatct
    20701 tgcaatttac tgcaaagcaa caacaaaatc gcaaagtcat caaaaaaccg caaagttgtt
    20761 taaaataaga gcaacactac aaaaggagat aagaagagca catacctcag tcacttatta
    20821 tcactagcgc tcgccgcagc cgtgtaaccg agcatagcga gcgaactggc gaggaagcaa
    20881 agaagaactg ttctgtcaga tagctcttac gctcagcgca agaagaaata tccaccgtgg
    20941 gaaaaactcc aggtagaggt acacacgcgg atagccaatt cagagtaata aactgtgata
    21001 atcaaccctc atcaatgatg acgaactaac ccccgatatc aggtcacatg acgaagggaa
    21061 agagaaggaa atcaactgtg acaaactgcc ctcaaatttg gcttccttaa aaattacagt
    21121 tcaaaaagta tgagaaaatc catgcaggct gaaggaaaca gcaaaactgt gacaaattac
    21181 cctcagtagg tcagaacaaa tgtgacgaac caccctcaaa tctgtgacag ataaccctca
    21241 gactatcctg tcgtcatgga agtgatatcg cggaaggaaa atacgatatg agtcgtctgg
    21301 cggcctttct ttttctcaat gtatgagagg cgcattggag ttctgctgtt gatctcatta
    21361 acacagacct gcaggaagcg gcggcggaag tcaggcatac gctggtaact ttgaggcagc
    21421 tggtaacgct ctatgatcca gtcaattttc agagagacga tgcctgagcc atccggctta
    21481 cggtactgac acagggattc gtataaacgc atggcatacg gattggtgat ttcttttgtt
    21541 tcactaagcc gaaactgcgt aaaccggttc tgtaacccga taaagaaggg aatgagatat
    21601 gggttgatat gtacactgta aagccctctg gatggactgt gcgcacgttt gataaaccag
    21661 ggaaaagatt catagccttt ttcatcgccg gcatcctctt cagggcgata aaaaaccact
    21721 tccttccccg caaaactctt caatgcctgc cgtatatcct tactagcttc cgcagaggtc
    21781 aatccgaata tttcagcata tttaacaaca tggatctcgc agataccgtc atgttcctgt
    21841 agggtgccat cagattttct gatctggtca acgaacagat acagcatacg tttttgatcc
    21901 cgggagagac tatatgccgc ctcagtgagg tcgtttgact ggacgattcg cgggctattt
    21961 ttacgtttct tgtgattgat aaccgctgtt tccgccatga cagatccatg tgaagtg
//
LOCUS       DABSYT020000075        21900 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.075, whole genome shotgun sequence.
ACCESSION   DABSYT020000075 DABSYT020000000
VERSION     DABSYT020000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.075"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            183..446
                     /gene="ykgM"
                     /locus_tag="ID725_RS16805"
                     /old_locus_tag="pgaptmp_003361"
     CDS             183..446
                     /gene="ykgM"
                     /locus_tag="ID725_RS16805"
                     /old_locus_tag="pgaptmp_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGV7851881.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            446..586
                     /gene="ykgO"
                     /locus_tag="ID725_RS16810"
                     /old_locus_tag="pgaptmp_003362"
     CDS             446..586
                     /gene="ykgO"
                     /locus_tag="ID725_RS16810"
                     /old_locus_tag="pgaptmp_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGV7851882.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(656..847)
                     /locus_tag="ID725_RS16815"
                     /old_locus_tag="pgaptmp_003363"
     CDS             complement(656..847)
                     /locus_tag="ID725_RS16815"
                     /old_locus_tag="pgaptmp_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851883.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            1672..2214
                     /gene="ecpR"
                     /locus_tag="ID725_RS16820"
                     /old_locus_tag="pgaptmp_003364"
                     /gene_synonym="matA"
     CDS             1672..2214
                     /gene="ecpR"
                     /locus_tag="ID725_RS16820"
                     /old_locus_tag="pgaptmp_003364"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGV7851884.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTYRRNAEAKLYSKLYKLVQ"
     gene            2289..2876
                     /gene="ecpA"
                     /locus_tag="ID725_RS16825"
                     /old_locus_tag="pgaptmp_003365"
                     /gene_synonym="matB"
     CDS             2289..2876
                     /gene="ecpA"
                     /locus_tag="ID725_RS16825"
                     /old_locus_tag="pgaptmp_003365"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGV7851885.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            2934..3602
                     /gene="ecpB"
                     /locus_tag="ID725_RS16830"
                     /old_locus_tag="pgaptmp_003366"
                     /gene_synonym="yagY"
     CDS             2934..3602
                     /gene="ecpB"
                     /locus_tag="ID725_RS16830"
                     /old_locus_tag="pgaptmp_003366"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGV7851886.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            3628..6153
                     /gene="ecpC"
                     /locus_tag="ID725_RS16835"
                     /old_locus_tag="pgaptmp_003367"
                     /gene_synonym="yagX"
     CDS             3628..6153
                     /gene="ecpC"
                     /locus_tag="ID725_RS16835"
                     /old_locus_tag="pgaptmp_003367"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGV7851887.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            6143..7786
                     /gene="ecpD"
                     /locus_tag="ID725_RS16840"
                     /old_locus_tag="pgaptmp_003368"
     CDS             6143..7786
                     /gene="ecpD"
                     /locus_tag="ID725_RS16840"
                     /old_locus_tag="pgaptmp_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGV7851888.1"
                     /translation="MRVNLLIAMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDTSGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNQGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            7755..8465
                     /gene="ecpE"
                     /locus_tag="ID725_RS16845"
                     /old_locus_tag="pgaptmp_003369"
     CDS             7755..8465
                     /gene="ecpE"
                     /locus_tag="ID725_RS16845"
                     /old_locus_tag="pgaptmp_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGV7851889.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVQPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            8778..9107
                     /gene="ykgJ"
                     /locus_tag="ID725_RS16850"
                     /old_locus_tag="pgaptmp_003370"
     CDS             8778..9107
                     /gene="ykgJ"
                     /locus_tag="ID725_RS16850"
                     /old_locus_tag="pgaptmp_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGV7851890.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(9355..9969)
                     /gene="yagU"
                     /locus_tag="ID725_RS16855"
                     /old_locus_tag="pgaptmp_003371"
     CDS             complement(9355..9969)
                     /gene="yagU"
                     /locus_tag="ID725_RS16855"
                     /old_locus_tag="pgaptmp_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HGV7851891.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            10387..11076
                     /gene="paoA"
                     /locus_tag="ID725_RS16860"
                     /old_locus_tag="pgaptmp_003372"
     CDS             10387..11076
                     /gene="paoA"
                     /locus_tag="ID725_RS16860"
                     /old_locus_tag="pgaptmp_003372"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HGV7851892.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            11073..12029
                     /gene="paoB"
                     /locus_tag="ID725_RS16865"
                     /old_locus_tag="pgaptmp_003373"
     CDS             11073..12029
                     /gene="paoB"
                     /locus_tag="ID725_RS16865"
                     /old_locus_tag="pgaptmp_003373"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HGV7851893.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            12026..14224
                     /gene="paoC"
                     /locus_tag="ID725_RS16870"
                     /old_locus_tag="pgaptmp_003374"
     CDS             12026..14224
                     /gene="paoC"
                     /locus_tag="ID725_RS16870"
                     /old_locus_tag="pgaptmp_003374"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGV7851894.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQIDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            14234..15190
                     /gene="paoD"
                     /locus_tag="ID725_RS16875"
                     /old_locus_tag="pgaptmp_003375"
     CDS             14234..15190
                     /gene="paoD"
                     /locus_tag="ID725_RS16875"
                     /old_locus_tag="pgaptmp_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HGV7851895.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(15369..16496)
                     /locus_tag="ID725_RS16880"
                     /old_locus_tag="pgaptmp_003376"
     CDS             complement(15369..16496)
                     /locus_tag="ID725_RS16880"
                     /old_locus_tag="pgaptmp_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7851896.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(16638..17696)
                     /locus_tag="ID725_RS16885"
                     /old_locus_tag="pgaptmp_003377"
     CDS             complement(16638..17696)
                     /locus_tag="ID725_RS16885"
                     /old_locus_tag="pgaptmp_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGV7851897.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            17942..18844
                     /locus_tag="ID725_RS16890"
                     /old_locus_tag="pgaptmp_003378"
     CDS             17942..18844
                     /locus_tag="ID725_RS16890"
                     /old_locus_tag="pgaptmp_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7851898.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
     gene            19081..19143
                     /locus_tag="ID725_RS16895"
                     /old_locus_tag="pgaptmp_003379"
     CDS             19081..19143
                     /locus_tag="ID725_RS16895"
                     /old_locus_tag="pgaptmp_003379"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851899.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
     gene            complement(19547..19825)
                     /locus_tag="ID725_RS16900"
                     /old_locus_tag="pgaptmp_003380"
     CDS             complement(19547..19825)
                     /locus_tag="ID725_RS16900"
                     /old_locus_tag="pgaptmp_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851900.1"
                     /translation="MSLYIKLILSIVREISVNTICSLIVVVALSLLSFSSVAKTITAV
                     GSTINSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(19992..20714)
                     /locus_tag="ID725_RS16905"
                     /old_locus_tag="pgaptmp_003381"
     CDS             complement(19992..20714)
                     /locus_tag="ID725_RS16905"
                     /old_locus_tag="pgaptmp_003381"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGV7851901.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
     gene            20813..21712
                     /locus_tag="ID725_RS16910"
                     /old_locus_tag="pgaptmp_003382"
     CDS             20813..21712
                     /locus_tag="ID725_RS16910"
                     /old_locus_tag="pgaptmp_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7851902.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
BASE COUNT         5593 a         5691 c         5537 g         5079 t
ORIGIN      
        1 tgtacgctta ccaatcactc atccaggcgt ttatgggagc gtcgccaatg gctgttaaca
       61 atgccagact tccccgttga ggaaattcca cataccacta atggtcacag ggattgggtg
      121 ttgaaatgat ccggatgagc atgtatcttt gtggttatgt tataacataa caggtaaaaa
      181 tgatgaagcc caatatccat cctgagtatc gtactgtggt gttccacgac accagtgttg
      241 atgagtactt taaaatcggc tcgactatca aaacagaccg tgagattgag ctggatggcg
      301 taacgtatcc gtacgtaaca attgatgtct cttcgaaatc gcacccgttc tatacaggga
      361 agctgagaac agtggcatca gaaggaaatg ttgcacgatt cacccaacgt tttggtcgtt
      421 ttgttagcac gaaaaagggg gcgtgatgaa agtccttaac tctctgcgta ccgcaaaaga
      481 acgccatcca gactgtcaga ttgtgaagcg aaaaggacgg ctatatgtga tttgtaaatc
      541 taatccacgc tttaaggccg ttcagggtcg taagaaaaaa cgttgattta aaattcgacg
      601 aattaacgat atttgtctga ttaataatca gaccggatta atgttggtgt atttattaca
      661 ccagcattaa ttttcctggg gatgtatctt cctgttcatt taaggtcaac tgcctgacgt
      721 ttctctccga atattccatt atattaatgt tgacttgttg accagcttcg cccctgtatg
      781 ctggcatcaa ccctctttta gactgaacac gccactcagt ttcctccctt tgcgacgcag
      841 cctgcattta cactcaaact gttaagatga taaatgtggt aaatctgttg gtactgacat
      901 aaaaacgttt acgccacagg aacggcctga tccaccggta accccgtcgc cgacgttcga
      961 gtgccagtta gagtaacgcg cacagatgac tgaatgcagt gccctggcaa agaggccatc
     1021 gttccggtga caactggcag tcttcgttta acttcactta atctggctct tagggggctt
     1081 accggacaga tgacgtactt acacctgttt aatttttcat cacttattgg gatgaacacc
     1141 cataaccatt ttgtgcggca tgttaatcca ttaaaacacc ttactgattg gcaaatcatc
     1201 tttaattatg actcatgata gtttcatatt ctatttctta tcatttagac ttgttttttt
     1261 actagtccat tacacaacac attaagacta ttcctaacac ctcagggcaa agttcctggc
     1321 taatataaaa tgcaagtaag aattgaacgt tatattgcca ataaccttat gaaactgaat
     1381 gtctttttct tcttatcaaa aaagcaatat tttcattttt tgtaaatatt gacttaacca
     1441 tggaattcat tttctgttca catattgaca ctcatcagga aaaaaacata aatttaaacc
     1501 taatcgaaat aattaaaaac ttaatctcgt ttaacctata ttgatatgtg ctacgtatct
     1561 tatttacttc cgatttacta aagaaactga atgtacctgt aaaaattaca ggtttggaaa
     1621 gtagtgacat ggcaaaatga ttacagcagg gactatgagg ttgaaaacca tatggaatgt
     1681 caaaaccgtt ctgataaata catctggtct ccccatgacg cctacttcta taaaggacta
     1741 tctgaactga ttgtggatat cgacagatta atttatctat cgctggagaa aatcagaaaa
     1801 gatttcgtgt ttatcaatct caatacggat tctttaactg aatttataaa ccgtgataat
     1861 gagtggttat ccgcggtaaa ggggaaacag gtcgtattga ttgcggccag aaagtcagaa
     1921 gccttagcaa attattggta ttacaacagc aatattaggg gcgtggtata cgctggactg
     1981 agtcgtgata ttagaaaaga actggcctat gtgattaatg gcaggttcct gagaaaagat
     2041 attaagaaag ataaaatcac tgaccgggaa atggaaatta tccgcatgac ggctcaggga
     2101 atgctgccta aatcgattgc cagaattgaa aattgtagtg taaagacagt gtatacctat
     2161 cggcgtaatg cagaggccaa gctgtactca aaattatata agttggttca gtaaactcca
     2221 ggcaagttag ttttaaaaaa tgactcactg ggacatcacg tcctcaattc aactcgggaa
     2281 gaaatacaat gaaaaaaaag gttctggcaa tagctctggt aacggtgttt accggcatgg
     2341 gtgtggcgca ggctgctgac gtaacagctc aggctgtagc gacctggtcg gcaacagcca
     2401 aaaaagacac caccagtaag ctggttgtga cgccactcgg tagcctggcg ttccagtatg
     2461 ccgaaggcat taaaggtttt aactcacaga aaggtctatt tgacgtggct atcgagggtg
     2521 actcaacggc taccgccttt aaactgacct cacgtcttat caccaacaca ttaacccagt
     2581 tggatacctc aggttccaca ctgaatgtgg gcgtggatta taacggcgcg gcagtcgaaa
     2641 aaactggcga taccgtgatg atcgataccg ccaacggcgt actgggcggc aaccttagcc
     2701 cgctggcaaa cggttacaat gccagcaatc gtaccaccgc acaggatggt ttcactttct
     2761 ccatcatcag cggcaccacc aatggtacca ccgcagtaac cgattacagc actctaccgg
     2821 aaggcatctg gagcggcgac gttagcgtac agttcgacgc gacctggacc agttaatctc
     2881 tctgatgtac cagcaggggt agccccccag ctttattcct ggacggaatt gttatgaaaa
     2941 agcaccttct gcttctcgct ctgctgttat ccggaatatc tccggcccag gcgctggatg
     3001 tcggcgatat atcatcgttt atgaacagtg acagcagcac gctgagcaaa acgatcaaaa
     3061 acagtaccga cagtggtcgc cttatcaata tccgtctcga acggctctct tcaccgcttg
     3121 acgacgggca ggttatctca atggacaagc cggacgagtt gctactcact cccgccagct
     3181 tgctgctacc cgcccaagcc agcgaagtga tccgcttctt ctataaggga ccagcagatg
     3241 aaaaagagcg ctactaccgc attgtctggt ttgatcaggc cctcagtgat gcgcagcgcg
     3301 ataatgccaa ccgcagcgct gtggccactg cttccgcccg catcggcacc attctggtcg
     3361 tcgccccccg ccaggcaaac taccactttc agtacgccaa cggcaccctg acaaatacag
     3421 gaaatgcgac gctgcggatc ctcgcctacg gcccctgcct gaaagccgcc aatggtaaag
     3481 agtgtaaaga gaattactac ctgatgccgg gcaagtcgcg tcgttttacc cgcgtggaca
     3541 cggcggataa caaaggacgg gttgcacttt ggcagggtga taagttcatt cccgtgaaat
     3601 agatagctgt gcagatggat aacgacaatg cctttacgac ggttctcccc aggactgaaa
     3661 gcccagtttg ccttcggcat ggtctttttg ttcgttcagc ccgatgccag cgctgctgac
     3721 ataagtgcgc agcaaatagg tggggtgatt attccgcagg ccttcagtca ggcgcttcag
     3781 gacggcatga gcgtcccgct ctatattcat ctcgccggta gccagggtcg ccaggacgat
     3841 cagcgaatcg gcagcgcttt tatctggttg gatgatggac agctacgcat ccggaaaata
     3901 cagctggaag agagtgaaga taacgccagt gtcagcgaac aaactcgaca gcagctgatg
     3961 gctctggcga acgccccgtt caatgaggcc cttaccatcc ccctgactga caacgcgcag
     4021 ctggatctca gcttgcgcca actgctgctg cagctggtgg tcaagcgcga agcgctgggc
     4081 actgtactac gctcacgtag cgaagacatc gggcagtcca gtgttaacac cctcagcagt
     4141 aatctgagct ataacttcgg catctataac aaccagttgc gtaacggcgg gagcaacaca
     4201 tccagctatc tgtcgctgaa taacgttact gcactgcgcg aacatcatgt ggtgctcgac
     4261 ggctcgctgt acgggatcgg tagcggtcaa caggacagtg aattatataa agcgatgtat
     4321 gaacgcgatt ttgccggtca ccgatttgcc ggtggaacgc tcgacacctg gaacttgcag
     4381 tccttagggc cgatgaccgc catttcagca gggaagattt acggcctttc ctggggaaac
     4441 caggccagct ccaccatctt cgacagcagc cagtcagcca cgccagtgat cgccttttta
     4501 ccggcggcgg gtgaagtaca tctcacccgt gatgggcggt tactaagcgt tcagaacttc
     4561 accatgggca atcatgaagt ggatacccgg ggtctaccat acggtattta cgatgtggaa
     4621 gttgaggtga tcgttaacgg tcgcgtgatc agcaaacgca cccagcgggt caataagctg
     4681 tttagccggg ggcgcggcgt cggtgcacca ctggcgtggc aggtatgggg cggtagcttt
     4741 catatggatc gctggtcgga aaacgggaaa aagacgcgac cagctaaaga gagttggctg
     4801 gcaggtgcct cgacctccgg ctcactgagt acgcttagct gggcggcaac gggatatgga
     4861 tacgataatc aggcggtggg tgaaacccgt ctgacgctgc cgcttggggg agcgatcaac
     4921 gttaacctgc aaaatatgct ggccagtgac agctcatgga gcagcatcgg cagcatcagc
     4981 gccactctac cgggaggctt tagttcgctg tgggttaatc aggaaaaaac ccgcattggc
     5041 aatcaattgc gacgtagcga tgccgacaac cgtgctatcg gcggcacact caacctgaac
     5101 tcactgtggt cgaagctggg cacattcagc atcagctaca atgatgaccg ccgttacaac
     5161 agccattatt acacggcaga ttactatcaa aatgtctaca gcggtacctt tggttcgctt
     5221 ggcctgcggg ccggtattca gcgctataac aacggcgaca gcaacgccaa tacagggaaa
     5281 tatatcgctc tcgatctctc gctaccactg ggcaactggt ttagcgcagg gatgactcat
     5341 caaaacggct acaccatggc aaacctgtca gcacgcaagc agtttgatga agggaccatt
     5401 cgcactgttg gtgccaatct gtcacgagcc atctccggcg ataccggtga tgacaaaact
     5461 ctcagtggtg gggcgtatgc acagttcgac gctcgctacg ccagcggaac gctgaacgtc
     5521 aatagcgcgg cggacggcta cgtcaatacc aatttaaccg ccaatggcag cgtcggctgg
     5581 cagggtaaaa acattgctgc cagcgggcgg actgatggca acgctggggt gatattcaac
     5641 accgggctgg aggacgacgg tcagatcagc gccaaaatca acgggcggat tttcccgctt
     5701 aacggcaagc gtaactatct cccgctctct ccctatggaa gatatgaggt ggagttacag
     5761 aacagcaaaa actcactcga cagttacgat atcgtcagcg gtcgcaaaag tcatctgact
     5821 ctctatccag gcaatgtcgc tgtcattgag ccagaggtga agcagatggt taccgtctcc
     5881 ggtcgtatcc gtgcggaaga cggcacactg ctggctaacg cacggattaa caaccatatc
     5941 ggccgaaccc gaaccgatga aaacggcgag tttgtcatgg acgtggataa gaaatacccc
     6001 actatcgatt ttcgctacag tggcaataaa acctgcgaag tggcactgga actcaaccag
     6061 gcgcgcggtg ccgtctgggt cggtgatgtg gtctgcagcg gcctctcatc gtgggcggcg
     6121 gtgacgcaga caggagaaga gaatgagagt taacctactg atagcgatga taatctttgc
     6181 gctaatctgg ccagtaactg cgctcagagc ggcagtgagc aaaacaacct gggcggatgc
     6241 accggcacgc gagtttgtgt ttgtcgaaaa caactcagac gacaactttt tcgtcactcc
     6301 tggcggggcg ctggatccgc gcctgaccgg tgccaaccgc tggaccggtt taaaatacaa
     6361 tggttcagga accatctatc agcaaagcct cggctacatt gataacggtt acaacaccgg
     6421 cctttatacc aactggaagt ttgatatgtg gctggaaaat tcaccagttt catctccttt
     6481 aactggcttg cgctgcatca actggtacgc tgggtgtaat atgaccacca gtcttatcct
     6541 gccgcaaacc accgacacca gtggatttta tggcgcgacg gtgaccagcg gcggcgcgaa
     6601 gtggatgcac ggcatgttgt cagacgcgtt ttaccagtat ctgcaacaaa tgcccgtcgg
     6661 cagcagcttt acaatgacca tcaatgcctg ccagacctct gtgaactatg acgccagcag
     6721 cggcgcacgc tgtaaggatc aggcctccgg caactggtat gttcgcaacg tcacccatac
     6781 gaaagcagca aatctacggt tgataaatac ccactcgctg gcggaagtat ttatcaacag
     6841 cgacggagta ccgactctgg gcgaagggaa cgccgactgc cggacgcaaa ccatcggcag
     6901 ccgttcagga ttaagttgta agatggttaa ctataccctg caaacaaacg gactcagcaa
     6961 cacctcaatc catatattcc cggcgatcgc caactcgtcg ttagcctcgg ccgtcggggc
     7021 gtacgatatg cagttcagtc tgaatggcag ttcatggaaa ccggtgagca atactgccta
     7081 ttactacacc ttcaacgaga tgaagagcgc agactcgatc tatgttttct tctcgagcaa
     7141 cttctttaag cagatggtga accagggaat cagcgatatc aacaccaaag atctattcaa
     7201 ctttcgcttt cagaacacca catcaccgga gtctggctgg tatgaatttt ctacctccaa
     7261 cacgctgatt atcaaacccc gtgatttcag catcagtatt atctccgatg aatatactca
     7321 gacaccgtcg cgggagggat atgtcggcag cggcgagtcg gcactcgatt tcggctatat
     7381 cgtaaccacc agcggtaaaa cagctgccga cgaagtgctg atcaaggtga ccggccccgc
     7441 gcaggtgatt ggcgggcgct cctattgtgt cttcagctcc gatgacggta aggcgaaagt
     7501 accgttcccg gcgacgcttt cctttattac ccgcaacgga gctacaaaaa cctacgatgc
     7561 cgggtgcgat gatagctggc gggatatgac cgatgcgctg tggttgacca caccgtggac
     7621 tgatatctct ggcgaagtgg ggcagatgga taagaccaca gtcaaatttt cgattccaat
     7681 ggataacgcc atttctctgc gtacggtaga tgataacggc tggtttggcg aagtcagcgc
     7741 ttcaggagaa attcatgttc aggcgacgtg gcgtaacatt aactaaggcc ctgctgacag
     7801 cggtctgtat gctggcggca cctttgacac aggcgatttc ggtcggcaat ctgacttttt
     7861 cactgccgtc cgagactgac tttgtcagca aacgtgtagt gaataacaac aaaagcgcgc
     7921 ggatataccg tattgccatc agtgctatcg atagcccggg cagcagtgaa ttacgcaccc
     7981 gaccggtgga tggtgaactg cttttcgccc cccgccagct ggcgttgcag gctggtgaga
     8041 gcgagtattt taaattttac tatcatgggc cacaggataa ccgcgagcgc tactaccggg
     8101 tctcatttcg cgaggtcccc actcgtaacc tgacaaaacg cagccctacc ggcggtgagg
     8161 ttagcacgga gccggtggtg gtgatggata ccattctggt agtacaacca cgtcaggttc
     8221 agtttaaatg gtccttcgat caggtgaccg gaacggtaag taacaccggc aacacgtggt
     8281 ttaagctact gattaaacca ggatgtgatt cgaccgaaga ggaaggcgat gcctggtatc
     8341 tacgtccgga agacgtggtt catcagcctg agttacgtca gccggggaat cactatctgg
     8401 tctataacga caaattcatt aagattagcg attcctgtcc ggctaagccc ccttcggcgg
     8461 actgatactt atccatcggt cgagggaaga attccacctc agagactcaa aaatcgttta
     8521 aatgatgtgg aagcgatcgt caatatcacc tgtaacgata ggctttctca tatgcacacc
     8581 acagtgttca ggagacacgg aaaggtacca ggcggcctgc taacgagtgg tttcttcact
     8641 aattaggacg taaaatcggt aacggctgga aatcattcaa tactcacact atcgaaagta
     8701 caccagccaa ccgcgatacg tccctgcata cgacgtaccg cagttctctt tacgatttat
     8761 agctgtactg gtgaattatg agcaatctga atccatgcat gacgtgtggt gcctgttgtg
     8821 catttttccg cgtctctttc tactgggccg aagccgacga tgctggcggc actattcccg
     8881 ccaggctcac tgaacaaata tccccttttc accgatgcat gagcggaact aatcagaaaa
     8941 acccccgatg tattgccctt gcaggaaccc cgggcaaaaa tgcctgctgc acgatatata
     9001 aaaatcgatc gtccacatgc agagaattcg ccatgtctgg tgagaacgga gtcgtcaatg
     9061 aggcttgcaa tcgtgcaagg gctaaatacg ggctgacacc gttataaata tacaataatt
     9121 atttgcactg ccccgccagc tgtaatagcg gggcttcgtt tttcaggggt aacaaacccc
     9181 gatacttctt ctatttgcca gcaacaatgc ctctcttctg tagcgttctt cggatctaca
     9241 tcatcctgaa gtagcgcgat atactcactg actatctgaa tcagaatata aaaagcaatg
     9301 tttttaacct ataaaaatgg cgctgtattt gcgccatttt tatcattcaa tgcattatct
     9361 gtttgagcct aaagggatct cagggtctgg ctcatgagta attctgtttc gtaaatctct
     9421 gcgaataatt tcaatggacc agaaccagac taaatgtcca aaaatttcag aaacattctc
     9481 ataccacggg agatcaaaca gaggtggtgt cagtcccatg agagggaatg aaatcatatg
     9541 aacaaaaagt tgggctaaag cacctgccag taagccctgc cagagtttaa tttttggaaa
     9601 tacttctgcg accacacaat aaccgacagc aaacactatc gaaaagataa tgtgcgtaac
     9661 accaacccag ttaaagacat gcccggcaaa ggtataaaca gccgcattgg gatctgtcag
     9721 ccccagccag tctcgaagaa aaatatacgg aggattgaga aaattacgcg agcaatcaat
     9781 ttgcccagca gccctgatta atgattccgg gccacacgct gcattaaaca tatccaccgg
     9841 gctacgtggc ggcaatggaa cttcagcccc ccacttcaca aatgcggaaa caacgccagc
     9901 aatcagccca atgaatgcag caagaccata acgtctgcgg ttcggtggag tttgttcaaa
     9961 tatattcata tctaccctgc ttgtaccatt atgttataca cctctttagg agtattcata
    10021 aaacaaggta aatgtaaaga actgtattgt tttgtataac aagatggttg tctaatcacc
    10081 aaagaatata agcaccatca tttctcccta tttttatatt agaagtgaca tagatttgca
    10141 gggtgatgca gagctgacaa tcacaggttt ccttatcggt ttttgcatcg tacaactaaa
    10201 gcaataaacc agctcccatc atcatcaaca tcccggcgaa catgaattta ttagccgggc
    10261 tcatagctgc attcgcgttt taagaataat cctcctgctg tcgccgacta tgcttaacgt
    10321 ttaaaaaagc atcagcactc tcgcaacgca ctcttatttt cccctttaga ataccggagg
    10381 cctggtatga gcaaccaagg cgaatacccc gaagataatc gggttgggaa gcatgagccg
    10441 cacgatttaa gtttgacccg tcgcgatctg attaaagtga gcgccgcaac ggcggcggcc
    10501 gccgtggttt atcctcattc tacgcttgcg gcaagcgttc cggcagctac acccgcgcct
    10561 gagataatgc ccctgacact taaggtgaac ggcaaaaccg agcagcttga ggtggatacc
    10621 cgaaccacgc tactggacgc tttgcgtgaa aatctgcatt tgatcggtac caagaaaggt
    10681 tgcgatcacg gacagtgcgg agcctgtacc gtgctggtca atggtcgcag gcttaatgcc
    10741 tgcctgacgc ttgcagtcat gcatcagggg gccgagatca ccaccattga aggcctgggt
    10801 tcaccagata atcttcaccc catgcaggcg gcctttatca agcatgatgg cttccagtgc
    10861 ggctactgca cctccgggca aatttgctca tcagtagcgg tgctaaaaga gattcaggac
    10921 ggcattccca gtcacgtcac ggtcgatttg gtttcccctc cagaaagaac tgccgatgag
    10981 atccgtgaac gtatgagcgg caacatctgt cgctgtggtg catacgctaa catccttgcc
    11041 gccattgaag acgctgcggg ggagataaaa tcatgaaggc gtttacctat gaacgagtga
    11101 acacccccgc cgaggcggca cttagcgctc agcgcgtacc cggcgcaaaa tttatcgcgg
    11161 gcgggaccaa tctgctggac ctgatgaagc tggaaattga aacgcccacc caccttatcg
    11221 atgtgaacgg gctcgggctc gataagatcg aagtgaccga cgcgggtggg ctgcgcatcg
    11281 gcgcactggt acggaacacc gacctggtgg ctcacgagcg cgtgcgtcgt gattacgcgg
    11341 tactctcccg cgccctgctc gctggcgcgt ctggtcagtt acgcaatcag gcaaccacag
    11401 caggtaatct gctccagcgc acgcgctgcc cctattttta cgacaccaat cagccctgca
    11461 ataagcgcct gcccgggagc ggctgcgcgg cgcttgaagg ctttagccgt cagcacgcgg
    11521 tggtaggcgt aagcgaagcc tgcattgcca cccatccgag cgatatggcg gtcgcaatgc
    11581 ggttgctgga tgcggtggtg gaaaccatca cgccggaggg aaagactcgc agtatcacac
    11641 tggctgattt ttatcaccca ccggggaaaa cgccgcacat tgaaaccgcc ctgcttcccg
    11701 gtgagcttat cgttgcggtg acgttacctc cgccgctcgg cggaaaacat atctaccgta
    11761 aggtgcgcga tcgcgcctcc tacgcctttg ccctggtatc ggtcgcggcg attattcagc
    11821 ctgacggcag cgggcgcgtc gcgctgggcg gagtagcaca taagccctgg cgcattgagg
    11881 ctgcggatgc tcagctatcc cagggggcgc aggccgtata tgacgcgctg ttcgccagcg
    11941 cccatcccac cgctgaaaac acctttaaac tcctgttggc gaagcgaacg cttgcctccg
    12001 tactggctga agcgagggca caagcatgaa atttgataaa cccgcagggg aaaacccgat
    12061 cgatcagctg aaggttgtcg gtcgtcccca tgaccgcatc gacggaccgc tgaaaactac
    12121 cggcacggca cgctacgcct acgaatggca tgaagaatcc cccaacgccg cctatggcta
    12181 tatcgtcggt tccgccattg ccaaaggacg cctcaccgcc cttgatacgg acgccgcgca
    12241 aaaagcgccg ggcgtactgc ctgtcattac cgccagtaac gccggggcac tcagtaaagg
    12301 cgacaaaaac accgccaggc tgttaggcgg ccccactatt gagcactatc atcaggccat
    12361 tgctctggta gtggccgaga ccttcgaaca ggcgcgagcg gcggcctcgc tggtgcaggc
    12421 gcactatcgc cgtaataaag gagcttactc cctggcggac gaaaaacagg ccgtcagtca
    12481 gccgccggaa gacacgcccg acaagaacgt cggtgacttt gacggggctt tctcctccgc
    12541 tgcggtgaag attgatgcta cctacacgac cccggaccag agccatatgg cgatggagcc
    12601 gcatgcctcg atggccgtct gggatggaaa taagcttact ctctggacct caaatcagat
    12661 gattgactgg tgccgcaccg atctggcaaa aacgctgaaa gtgcccgtgg agaatgtgcg
    12721 tattatctcc ccgtatatcg gcggagggtt tggcggcaag ctgttcctga gaagcgatgc
    12781 gctgctggcg gccctcgccg cccgagcggt gaaacgtccg gttaaagtga tgctcccccg
    12841 cccctctatt cccaataaca ccacgcaccg ccccgccacc cttcagcact tgcgtatcgg
    12901 tgccgaccag agcgggaaaa tcaccgctat ctcacatgaa agctggtctg gaaacctgcc
    12961 cggcggcacg ccggaaacgg cggtacagca aagcgaatta ctctacgccg gggcaaaccg
    13021 tcataccggc ctgcggctcg ccacgcttga tttgccggaa gggaacgcca tgcgtgcgcc
    13081 cggcgaagcc cccggtctga tggcgctcga aatcgcgatc gacgaactgg cggaaaaagc
    13141 gggcatcgat cccgtcgagt ttcgcatcct gaatgacact cagattgacc ccgccgaccc
    13201 gacgcgccgc ttctctcgcc gtcagcttat cgagtgcttg cgcaccggag cggataaatt
    13261 tggttggaag cagcgcaacg ccacacccgg acaggtgcgc gacggggagt ggctagtcgg
    13321 ccacggcgtc gcggcgggct ttcgcaataa tctgctggaa aaatcggggg ctcgggttca
    13381 cctcgaacca aacggcaccg ttaccgtgga aacggacatg accgacattg gcaccggcag
    13441 ctacaccatt ctggcccaga cggcagcgga aatgcttggc gtaccgctgg agcaggttgc
    13501 ggttcacctc ggcgattcca gtttcccggt ttctgcgggt tctggtggac aatggggcgc
    13561 gaatacctcc acctccggcg tttacgccgc ctgtgtgaag cttcgcgaaa tgattgcctc
    13621 ggcagtcggg tttgatcctg agcagtcgca gtttgccgac ggcaagatta ccaacggtac
    13681 ccgaagcgcc atactacatg aggccaccgc aggcggcaga ctgacagcgg aagagagcat
    13741 tgaattcgga acactgagca aggagtacca gcagtcgacc tttgccgggc attttgtgga
    13801 ggtcggcgtg catagcgcga cgggagaagt tcgggtccgg cgtatgctcg ctgtgtgtgc
    13861 tgcaggacgc atcctgaatc cgaaaactgc acgcagccaa gtcattggcg caatgactat
    13921 gggcatgggc gcggcactga tggaggagct ggcggtggat gaccgtttgg gctacttcgt
    13981 taatcacgat atggcggggt atgaggtgcc ggtccatgcg gatatcccaa aacaggaagt
    14041 gattttcctg gatgataccg accccatatc ctccccgatg aaggccaaag gtgtcggtga
    14101 gctgggcctg tgcggcgtga gcgcggctat cgccaacgcg gtgtataacg ccaccggtat
    14161 tcgggtacgc gattatccca tcactctgga taagctgctc gataagctgc cggatgtggt
    14221 ttaaggagga acaatgtcat acccgctttt tgataaagac gaacactggc ataagccaga
    14281 gcaggcgttt ctcaccgatg accaccggac cattctgcgc ttcgccgtag aggcgctaat
    14341 gtccggtaaa ggagcggtgc tggtgacgct ggtggagata cgcggcggcg cggcgcgccc
    14401 gctcggggcg cagatggtgg tgcgcgaaga tggtcgttac tgcggttttg tctctggcgg
    14461 ctgcgtggag gccgctgccg cttttgaagc gctggagatg atgggctcag gctgcgatcg
    14521 cgaaattcgc tatggcgaag gttcgccgtg gtttgacatc gttctgccct gcggcggtgg
    14581 gatcacgctg acgctccata aactacgctc ggcacagcct ctgctcgccg tgctgaaccg
    14641 cctggaacag agaaaaccgg cggggctgcg ctacgatccg caagcacaat cgttggtgtg
    14701 cctgcccacg caaacccgaa cgggctggaa tctcaatggc tttgaggtgg ggttcaggcc
    14761 atgcgtcagg ctgatgattt acggacgttc tcttgaggcg caggcaaccg cgagtcttgc
    14821 agcagccaca ggctatgaca gccatatctt cgatcttttt ccggcctcag ccagcgctca
    14881 gatcgatacc gatacggcgg tcattttgct gtgccatgat ctcaaccggg agctgccagt
    14941 gttgcaggcc gcgcgagaag caaaaccctt ttatctcggc gcattgggca gctatcgaac
    15001 ccacacgtta cgtctgcaaa agctccacga gctgggatgg tccagggagg agacagcgca
    15061 aatccgggca cccgtcggga tattccccaa agcccggaat gcgcatacac tggccctctc
    15121 cgtgctggca gaagtcgcct ctgtacgtct ccatcaggag gaggattcat gcctgccccc
    15181 gtcgtcctga tccttgcggc cgggcgtgga aagcgctttc tcacctccgg gggaaatacc
    15241 ataaattaag agaaaaattt gcagtagccg ttttagaaaa tcacctaaat cgtttaaaag
    15301 agcgccctac ccatagtggt tgctcatcgc cttgtgtaac tttttcagca acgcgcgccc
    15361 tcgccggact aaagcgcacc ggccttctgg cgtgctgtaa caaaggtcag tacgcccgcg
    15421 caaaggagca gcacactact catggcaaac gtactctgcc agccaaggcg gtcaaacacg
    15481 atgcctcctg ccgttgatcc aagggcgatt gagagctgaa tcaccgcgac catgagcccg
    15541 cctccggctt cggcgttgtc aggcaacgta cgcgcaatcc atgtccacca tccggttggc
    15601 gcggcggtgg ccagcatgcc ccagagtcct aacagcagcg agaccatcca gacgtgatgc
    15661 ccggtaagca tcagaccgat ggcaatgacg gccatcagtc cgggtatggc catcagcgtg
    15721 gggtagaatt ttctgttgag aaatatcgaa acaaccagcg tgccaataaa accggccacg
    15781 ccgataatca gcaaaatcaa cgacaagcct aaggaattaa cccgtgtcac cgactccaga
    15841 aatgggcgca cataggtgaa tagcgcaaac tgccccatga aaaacaggcc acaagctatc
    15901 atgccgacca caaccatgcg gcggctaaat aagcggaata ccgcgccaca tgacgtgtgg
    15961 cttttattcg catccatgct gggcagacta atgcactgcc agataaaggc gacaaccgcc
    16021 atgggcacca agcacaagaa agcgcctcgc catccgaccg tagcccccag atagcttccc
    16081 agcggcgcgg ccacgaccgt cgccagcgca ttaccggcgt tgaaaatcgc cagcgcacgc
    16141 gtgacctgat gctgcggcac cagacgaatc gccgttgccg ctgacatcga ccagaatccg
    16201 ccaatggcca caccaatcat cgcacgaccg accatataca tcagataact ggtagccagc
    16261 gcaataataa gccccgatac ggccatcaga accgtcatcc ccagcagcag gaacttacga
    16321 ttcatctttc cagctagcgt tgaaagcgtc aggctggtca gaaccgccag cgcaccggag
    16381 atagcaatcc cccgccctgc cagcccctcc gtcacgccca ggtcacgggc aatcggcgtg
    16441 agcaggctga cgggcataaa ttcagaagca atcagcacaa acacgcacag ggtcatggca
    16501 aaaataccgc cccagtatgc gtgctgatga tgaggtgatg gtgtttggct tagtgtcgac
    16561 atagtgaaat ttcacaaaga tcaggataaa agccaccggc catagaccgg tggctttgca
    16621 tcaattcagg ggaggtttta tttcagtttg gtctggaaaa attgttcgaa cttcgcgaaa
    16681 ggtatttttc cggccacgtt atcgtacagg tccacgtgat ttgcccctgg taccaccacc
    16741 aactctttgt ctttactgcc aaccgccttg taagcatctt cagcaaaata gcgtgaatgt
    16801 gctttctcac cggtcacaat cagcgtcgga atggtgattt cactggcgta gctcaacagc
    16861 ggcatattca taaatgacag cggcatcgtt gccgtccacg ccccggtaga gttaacggaa
    16921 cgttcgtgga agccgcgcgg catgcgatag tagtcaaaga actctttcag taccggatga
    16981 ggatttgccg gcagcgtttc tggcagaatg cgctcgcccg cactgacctt gccgttgcta
    17041 tcgacattaa tatcatgacc accgtgagcg aacgttccgc tctgcgcatc cttccagcgc
    17101 tgttcattca aatattgcag aacagcacga cggtcggcgg tagaataacg gtcttcgcca
    17161 tcccccacac catggcccat cgcccggctc atgtcataca tcacgctggt agcaaccgct
    17221 ttaacgcggg tatccatcgc cgcgtcgttc agcgccatgc cgccccagcc acaaataccg
    17281 agcagcccga tacggttgcg atctacctct ttttgcagcc ctaagaaatc caccgccgcg
    17341 ctgaaatctt cagtgttgat atccggtgag gcgacatttc gcggatagcc gccgctttcc
    17401 ccggtgtaag aaggatcgaa cgccagggta acaaaccctc gttccgccag cgtctgcgca
    17461 tacaagccgc tggattgctc tttcaccgcg ccaaatggcc cactgaccgc aatcgccgcc
    17521 agcttgcgat cgccacggtc tttaggcagg taaagatcgc ccactaaggt gatgccatat
    17581 cggttcggga acgagacttt acggtgatcg actttctggc tctcggcgaa cgttttatcc
    17641 catttatcgg tcatggacac aggggcatta ggattggttg aatcagcata actcattgtc
    17701 gtgactccac ttaatgatgc gaaaagcagc atgaccggca tcgcggtttt tagcgttcgg
    17761 gtgaaggctt tcataaaaat ccccataaaa aacaacagag agatacaggt ttccgacgga
    17821 caacgctgcg gtgctttact ttgatggggt cattatagtg agcagaatta gtgctgatta
    17881 gggggcgatt aatgctaaga ttaataccat attgttataa attaatgctg agattgccgg
    17941 catgaagggg aatgtttcaa tggcaaaaag ggagaactac aacgagctat acctgtttat
    18001 gcaggtggtg cgggaaggca gctttaccgc agcggctaag cggctgggtc ttgctcagtc
    18061 aggagtcagc cgttctgttc gcgagcttga agaaaggctg ggcgtccagc tgctggtgcg
    18121 caccacgcgt aaactgtcgc tgacgcaagc aggcgagcag ctctaccaga agacggcatc
    18181 tggatttgaa atgttagatc ttgggcttgc cacgctggcg cactatcgag agacgccctc
    18241 cggcacggtg cgtatcaatg ccagccagca cgcgattgat aaatgcctgc tgccaaagct
    18301 ggcggtattt aaacagcgat atcctgatat ccagcttgaa ctgataaatg agagccggtt
    18361 cgtcgatatc atcgaggaaa gattcgatgc aggtgtccgc ctggggcctg aagtcagcca
    18421 gggcatggtc gcggtacgca tcacgcccga tatggagatg gcaattgtgg ggactccgga
    18481 acattttcgt cgctatggtt ttccgcaaac cccggcggat ttaaaggcgc atccctgtat
    18541 cgcctatcag tttgccgacg gcagcgtata ccagtgggaa ctcaatcagg atgataaaaa
    18601 aatcactcat caacccgaag gacaatgggc cttttccgac agctatatgg aagcagaagc
    18661 cgcccggctg ggcctcgggt tggcctatgt tcctgttgaa ctggtcgcgg atgatttaga
    18721 acacggtaag cttatcagag tgttgcagcg ttacagcctg cgaatggagg gattgtttct
    18781 ctattaccct catcgcaacg tctcccccgc tctgcggatg gtcattgata cattgaaaat
    18841 ctgaacgtta acaacgaccg actcttcact gtgcaatatt gtgtcaggta gtcactctgc
    18901 gttcaattcc aggggccgct ttgagcgaga aagagcggac ttatctgtac aaggggacta
    18961 tccagcaatg gagatcatcc aaaagaaaaa accacctggc tcccctcgac aaccttaatc
    19021 cgggcattta gcctcaatca acattttcat atctgtcagg ttacttcatt atgaaaaccg
    19081 atcaataccg ttgtttttgt tgcaacagta gaatacgcgt atttatccct ccatttctat
    19141 taataacggc aataaactgt tcatttcact gatatttaaa atatgcatcc ttttcaagct
    19201 gttcagcggc gtaagaaaac gtattaaata tgaattcgac gatttaacca tttctgtctg
    19261 attggtaatc atctgtgatt aatgctggtc tattgattac tccagcatta accgcttagg
    19321 gatgtattct tcctgttgat atacagccct cgaaaaagag agaaaataaa actgtgtcca
    19381 atattactct accactccag accgaagcat cggtagaatt agcagaacga ctggtacaat
    19441 agattgggat agcgtcattt gtcaggtatt ccattgcctg acaaatgaca attttgagtc
    19501 aatagacgtg cttcttgatc atgtcttaaa aattatacca tgagccttat gaagcatcat
    19561 ttttactgac tggtgttaac tttgctatca tataaactct gttcttaaaa aacgcgccca
    19621 gaattttata tgatttccct tgtttttctg cctgtaaaga aatttctttt tcagtgctgt
    19681 taatggttga accgacggca gtaatcgttt tggcgacgct actgaatgat aataaagaca
    19741 gtgcaaccac aacaattaaa gaacagatag tattcacgct aatttccctg acaattgaga
    19801 ggatgagttt tatatataaa gacattaaac atgagtcatg tttacttcct gtgaaaaata
    19861 atctttctgt aattaaacct tcatctcctt cggggtgagc aatacataat gtatcttgct
    19921 atgaaaaaca cagggatata taaatattcc ttctgaccta tttaataaaa cgtgaatgta
    19981 aaagttattc ttcagtttgc agatgcagtg ggtctgatgg taatttcggt tgtatcaacg
    20041 ctttcgggag attcaataat atgccttaca gcacgggcaa tatccgcggg ttgaagtgca
    20101 acagaacggt aggcgtccat tgccttcatg gtttccacat gggtaatggt tgaagccagt
    20161 tcactttcga caacgccagg atttacgcag gtcacacgga ttttcgagct ttcctgccgt
    20221 aagccatcag aaatagcccg tactgcaaat tttgatgcac aatagactgc gcctgtgggc
    20281 acaacagaaa gggcaccaat agaaccaaga ttgattatct gaccggaacc ctgtgcttcc
    20341 atcaccggaa gtacagcgcc aattccccac agtacacctt tgatattcac gtcaatagtg
    20401 agcgcccatt catcttgttt tcctgctgca agcggtgaaa gcggcataac gcccgcatta
    20461 ttgataagaa catcaactcg cccccagcta tccagcgctg cttgcacgaa atcggccatg
    20521 gactgtcggt ctgtgacatc caactcccgc gctttagcaa ttcctcctgc gcggcagatt
    20581 tcggttgcaa tggcttcgat acgctcaact ctgcgtgctc ccagtaaaac ctttgcacct
    20641 gtcattccaa gctctctggc aataccttcc ccaataccac ttgatgcgcc ggtaattaat
    20701 atgaccttat ccattacgtt ctccttcaaa tgtgttcgga gagagaatag aactttatga
    20761 ttggttccag tatcctgctt tcaatgtact cagtggttag cggcattaaa caatgaaaat
    20821 cgatcttaat cttttaccca tttttatcgc ggtagcagaa gagagtaatt ttagtaaagc
    20881 cgccgcacgg ctgggcgtta cccgttcagc ggtcagtcag ggaataagac gccttgaaga
    20941 tgcattcggg acaatgcttg taatgcggac cacgcgctca gttaatttga cagaagcggg
    21001 agaacggctg cataaatcgc tgtcattacc tttatccggt attgaggcag cgttcgaaga
    21061 gatggtttct gaccatgtgc cacgcggact tctcagaatc gcggtgacgt caatagctga
    21121 agaatttctt tcaggtccgc taatcgcttc gtttgcagca gccaatccag ccgttacgct
    21181 tgatattgtt gtcacggatg aggaattcga tatcgtggcc gcaggttacg atgctggcgt
    21241 aaggctcgga gaggtcattg aaaaagacat gattgctgtg ccccttaccg gcaggcagcg
    21301 tgaaatggtc gttgcctctc cctcctatct cgcagccaac agcacacctg ttcatccgcg
    21361 tgaacttgtg aatcataagt gtatcggctg gcgtcagtcc ccggaggttg cgccttatcg
    21421 ctggccattt gaagaaaacg ggagagcttt cgaccttgcg attgaaccgc agattacgac
    21481 taatgatctg cgtctgatgt tgaggctggc tcttgccggc ggaggaataa caattgccac
    21541 tcaggaaact ttcaggccgt atattgaaag cggtaagctt gtatctctgc ttgatgactt
    21601 tcttccacaa tttccgggct tctatctgta tttcccacag cgtcgcaata ttgcaccaaa
    21661 gctccgcgct ctgattgacc acgttaagga atggcggcag caattggctt aaatgtctgc
    21721 acctgcattg cctgatgtca gaacagtatt ttgattaatt gccagggtta caatggcaca
    21781 aatgcggcac aggaggaaac gtggtgtatt taaatatggg gtaactactt gatttaaatg
    21841 gtgcgataat aggaaccaat aatcgccacc aacacaatga ttttaataca taaattaaaa
//
LOCUS       DABSYT020000076        21840 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.076, whole genome shotgun sequence.
ACCESSION   DABSYT020000076 DABSYT020000000
VERSION     DABSYT020000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.076"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            173..508
                     /gene="yjdM"
                     /locus_tag="ID725_RS16915"
                     /old_locus_tag="pgaptmp_003383"
     CDS             173..508
                     /gene="yjdM"
                     /locus_tag="ID725_RS16915"
                     /old_locus_tag="pgaptmp_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGV7851903.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            767..1210
                     /gene="yjdN"
                     /locus_tag="ID725_RS16920"
                     /old_locus_tag="pgaptmp_003384"
     CDS             767..1210
                     /gene="yjdN"
                     /locus_tag="ID725_RS16920"
                     /old_locus_tag="pgaptmp_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGV7851904.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1343..2131
                     /gene="phnC"
                     /locus_tag="ID725_RS16925"
                     /old_locus_tag="pgaptmp_003385"
     CDS             1343..2131
                     /gene="phnC"
                     /locus_tag="ID725_RS16925"
                     /old_locus_tag="pgaptmp_003385"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGV7851905.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2156..3172
                     /gene="phnD"
                     /locus_tag="ID725_RS16930"
                     /old_locus_tag="pgaptmp_003386"
     CDS             2156..3172
                     /gene="phnD"
                     /locus_tag="ID725_RS16930"
                     /old_locus_tag="pgaptmp_003386"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGV7851906.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            3278..4057
                     /gene="phnE"
                     /locus_tag="ID725_RS16935"
                     /old_locus_tag="pgaptmp_003387"
     CDS             3278..4057
                     /gene="phnE"
                     /locus_tag="ID725_RS16935"
                     /old_locus_tag="pgaptmp_003387"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGV7851907.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4078..4803
                     /gene="phnF"
                     /locus_tag="ID725_RS16940"
                     /old_locus_tag="pgaptmp_003388"
     CDS             4078..4803
                     /gene="phnF"
                     /locus_tag="ID725_RS16940"
                     /old_locus_tag="pgaptmp_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGV7851908.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4804..5256
                     /gene="phnG"
                     /locus_tag="ID725_RS16945"
                     /old_locus_tag="pgaptmp_003389"
     CDS             4804..5256
                     /gene="phnG"
                     /locus_tag="ID725_RS16945"
                     /old_locus_tag="pgaptmp_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGV7851909.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5253..5837
                     /gene="phnH"
                     /locus_tag="ID725_RS16950"
                     /old_locus_tag="pgaptmp_003390"
     CDS             5253..5837
                     /gene="phnH"
                     /locus_tag="ID725_RS16950"
                     /old_locus_tag="pgaptmp_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGV7851910.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5837..6901
                     /gene="phnI"
                     /locus_tag="ID725_RS16955"
                     /old_locus_tag="pgaptmp_003391"
     CDS             5837..6901
                     /gene="phnI"
                     /locus_tag="ID725_RS16955"
                     /old_locus_tag="pgaptmp_003391"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGV7851911.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6894..7739
                     /gene="phnJ"
                     /locus_tag="ID725_RS16960"
                     /old_locus_tag="pgaptmp_003392"
     CDS             6894..7739
                     /gene="phnJ"
                     /locus_tag="ID725_RS16960"
                     /old_locus_tag="pgaptmp_003392"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGV7851912.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7736..8494
                     /gene="phnK"
                     /locus_tag="ID725_RS16965"
                     /old_locus_tag="pgaptmp_003393"
     CDS             7736..8494
                     /gene="phnK"
                     /locus_tag="ID725_RS16965"
                     /old_locus_tag="pgaptmp_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGV7851913.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8605..9285
                     /gene="phnL"
                     /locus_tag="ID725_RS16970"
                     /old_locus_tag="pgaptmp_003394"
     CDS             8605..9285
                     /gene="phnL"
                     /locus_tag="ID725_RS16970"
                     /old_locus_tag="pgaptmp_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGV7851914.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9282..10418
                     /gene="phnM"
                     /locus_tag="ID725_RS16975"
                     /old_locus_tag="pgaptmp_003395"
     CDS             9282..10418
                     /gene="phnM"
                     /locus_tag="ID725_RS16975"
                     /old_locus_tag="pgaptmp_003395"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGV7851915.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10418..10975
                     /gene="phnN"
                     /locus_tag="ID725_RS16980"
                     /old_locus_tag="pgaptmp_003396"
     CDS             10418..10975
                     /gene="phnN"
                     /locus_tag="ID725_RS16980"
                     /old_locus_tag="pgaptmp_003396"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGV7851916.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10962..11397
                     /gene="phnO"
                     /locus_tag="ID725_RS16985"
                     /old_locus_tag="pgaptmp_003397"
                     /pseudo
     CDS             10962..11397
                     /gene="phnO"
                     /locus_tag="ID725_RS16985"
                     /old_locus_tag="pgaptmp_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            11399..12157
                     /gene="phnP"
                     /locus_tag="ID725_RS16990"
                     /old_locus_tag="pgaptmp_003398"
     CDS             11399..12157
                     /gene="phnP"
                     /locus_tag="ID725_RS16990"
                     /old_locus_tag="pgaptmp_003398"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGV7851917.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12234..12563)
                     /gene="yjdP"
                     /locus_tag="ID725_RS16995"
                     /old_locus_tag="pgaptmp_003399"
     CDS             complement(12234..12563)
                     /gene="yjdP"
                     /locus_tag="ID725_RS16995"
                     /old_locus_tag="pgaptmp_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGV7851918.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12632..14902)
                     /locus_tag="ID725_RS17000"
                     /old_locus_tag="pgaptmp_003400"
     CDS             complement(12632..14902)
                     /locus_tag="ID725_RS17000"
                     /old_locus_tag="pgaptmp_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGV7851919.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            15016..16536
                     /locus_tag="ID725_RS17005"
                     /old_locus_tag="pgaptmp_003401"
     CDS             15016..16536
                     /locus_tag="ID725_RS17005"
                     /old_locus_tag="pgaptmp_003401"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGV7851920.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16533..17507
                     /locus_tag="ID725_RS17010"
                     /old_locus_tag="pgaptmp_003402"
     CDS             16533..17507
                     /locus_tag="ID725_RS17010"
                     /old_locus_tag="pgaptmp_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGV7851921.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGSNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            17526..18467
                     /locus_tag="ID725_RS17015"
                     /old_locus_tag="pgaptmp_003403"
     CDS             17526..18467
                     /locus_tag="ID725_RS17015"
                     /old_locus_tag="pgaptmp_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGV7851922.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18522..19205
                     /locus_tag="ID725_RS17020"
                     /old_locus_tag="pgaptmp_003404"
     CDS             18522..19205
                     /locus_tag="ID725_RS17020"
                     /old_locus_tag="pgaptmp_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HGV7851923.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19220..20080
                     /locus_tag="ID725_RS17025"
                     /old_locus_tag="pgaptmp_003405"
     CDS             19220..20080
                     /locus_tag="ID725_RS17025"
                     /old_locus_tag="pgaptmp_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HGV7851924.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20077..21078
                     /locus_tag="ID725_RS17030"
                     /old_locus_tag="pgaptmp_003406"
     CDS             20077..21078
                     /locus_tag="ID725_RS17030"
                     /old_locus_tag="pgaptmp_003406"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGV7851925.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHITHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21075..21782)
                     /locus_tag="ID725_RS17035"
                     /old_locus_tag="pgaptmp_003407"
     CDS             complement(21075..21782)
                     /locus_tag="ID725_RS17035"
                     /old_locus_tag="pgaptmp_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGV7851926.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
BASE COUNT         4752 a         6442 c         6193 g         4453 t
ORIGIN      
        1 tggaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg atcgtcgtct
       61 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
      121 caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt
      181 accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca tgtacatctg
      241 cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat
      301 cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga
      361 tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga aaaacatccg
      421 cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact
      481 gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg gataaggcgt
      541 ttacgccgca tccggcaaca gtgccagatg cctgatgcga cgctcgcgcg tcttatcagg
      601 cctacaaatt cccgcaccct ccgtaggccg gataaggcgt ttacgccgca tccggcaaca
      661 gtgccgactg cctgatgcga cgctcgcgcg tcttatcagg cttccctctc atctgtataa
      721 atttcgaact acacttaact ggcttctctt aactgaggtc accatcatgc cgttaagtcc
      781 ctacctctct tttgccggta actgctccga cgcgattgcc tattatcaac gtacgttggg
      841 cgctgaactg ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc
      901 cgagaactgc ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaatgtgcg
      961 cattgccgga agcgatatca tgatgagcga tgctataccg tcaggaaaag ccagctactc
     1021 cggttttacg ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgataa
     1081 ccttgccgct aacggaaaaa tcgaaatggc ctggcaggaa actttctggg cgcatggctt
     1141 tggcaaagtc accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc
     1201 aacgcaataa tccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc
     1261 ttcatctgtt agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc
     1321 acttttttag ggaggctgca tcatgcaaac gattatctgc gtcgaacaac tgagcaaaac
     1381 cttcaaccag catcaggcgc tgcacgcggt tgatctgaat atccatcatg gtgaaatggt
     1441 ggctctgctt gggccgtccg gttccggcaa atccaccctt ttacgtcact taagcggttt
     1501 gattaccggc gataaatccg ccggtagcca tatcgagctg ctgggccgca ctgtccagcg
     1561 cgaaggccgc ctggcccgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca
     1621 acaattcaac ctggtgaatc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg
     1681 cagcacgccg ttctggcgca cctgttttag ttggttcacc cgcgagcaga aacagcgcgc
     1741 attacaggcg ctgacccgcg tcggtatggc gcactttgcg tatcagcgtg tttccacgct
     1801 ctccggcggc cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaagt
     1861 gattctcgct gacgaaccga tcgcctcgct ggacccggaa tcggcgcgca tcgtgatgga
     1921 caccctgcgc gacatcaacc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt
     1981 ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgtcttcta
     2041 cgacggcagc agccaacagt ttgataacga acgttttgac catctctacc gcagcatcaa
     2101 ccgcgtcgaa gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa
     2161 cgctaagata attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag
     2221 cccggcacac gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc
     2281 acagcaaaac ctgaaaccgc aatggacgcc gtttttacag gatatggaga agaagctggg
     2341 cgtgaaggtc aacgccttct ttgccccgga ctatgcgggc attatccagg gaatgcgttt
     2401 caacaaagtg gatatcgcct ggtacggcaa cctgtcggcg atggaagcag tggatcgcgc
     2461 caacggccaa gtcttcgctc agaccgtcgc ggcggatgga tcgccgggtt actggagcgt
     2521 gttgatcgtc aacaaagaca gcccgatcaa caacctgaac gatctgctgg cgaagcggaa
     2581 agatctcacc ttcggtaatg gcgatcctaa ctccacctct ggcttcctcg tccccggcta
     2641 ctacgtcttc tccaaaaaca atatctccgc cagtgacttc aagcgcaccg tcaacgccgg
     2701 gcatgaaacc aacgcgctgg ccgtcgccaa caagcaggtg gatgtggcga ccaacaacac
     2761 cgaaaacctc gacaagctga aaacctccgc gccggagaag ctgaaagaac tgaaagtgat
     2821 ctggaagtca ccgctgatcc caggcgatcc gatcgtctgg cgtaaaaacc tttccgaaac
     2881 caccaaagac aagatctacg acttctttat gaattacggc aagacgccgg aagagaaagc
     2941 ggtgctggaa cgcctgggct gggcgccgtt ccgcgcctcc agcgacctgc aactggtgcc
     3001 gattcgccag ctcgcactgt ttaaagagat gcaaagcgtg aaaggcaata aagggctgaa
     3061 tgagcaggac aagctggcaa aaaccaccga gattcaagcg cagctggatg acctggaccg
     3121 cctgaacaac gcgttaagcg cgatgagttc ggtaagtaaa gcgatgcagt aaatcgtagg
     3181 tcggataaga cgccccggtg tcgcatccga caatgtgcag gcgttgatgc cggatgcggt
     3241 gcaagcacct tatccggcct acagaccgga gccaaacatg caaaccatca ccatcgcccc
     3301 acccaagcgc agctggttct cgcttctgag ctgggccgtt gtactcgccg tgttggtcgt
     3361 ctcgtggcag ggcgcggaaa tggccccgct cacgctgatc aaagacggcg gcaacatggc
     3421 gaccttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt acctcaccga
     3481 aatgctcgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcggtgg ttctctccat
     3541 ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc agcccgtgcg
     3601 ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc tgttcgtggt
     3661 cgccgtcggc ctcggcccat tcgctggtgt gctggcgctg tttatccaca ccaccggtgt
     3721 gctctccaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg tggaaggcat
     3781 tcgcgccacc ggcgccaaca agctcgaaga gatcctctac ggcgtgctgc cgcaggtgat
     3841 gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct cggcgaccgt
     3901 cgtcggcatg gtcggcgcgg gcgggatcgg cgtcacgctg tgggaagcga ttcgcggttt
     3961 ccagttccag caaacctgcg ccctgatggt gctgattatc gtcacggtca gcctgctgga
     4021 tttcctctct caacggttgc gtaagcactt tatctgatta gcgaggcatt gatagctatg
     4081 cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc cgcaaaactt
     4141 gagcaggagc ttcgtcaaca ctaccgctgc ggcgactatc ttcccgccga gcagcagctg
     4201 gcggcgcgct ttgaggtaaa tcgccacacc ctgcgccgcg ccatcgacca actggtggaa
     4261 aaaggctggg tacagcgccg tcagggcgtc ggcgtgctgg tgctgatgcg cccgttcgat
     4321 tacccgctca acgcccaggc gcgttttagc cagaatctgc tggatcaggg cagccatccc
     4381 accagcgaaa aactgctgtc tgtgctgcgc ccggcctccg gtcacgtcgc tgacgcgctg
     4441 gggataaccg agggggagaa cgtcatccat ctgcgcaccc tgcgccgggt caacggcatc
     4501 gcgctctgtt taatcgacca ctacttcgcg gacctcaccc tctggccgac gctgcaacgc
     4561 ttcgacagcg gctcgctgca cgattttctg cgcgagcaaa cggggattgc actgcgccgc
     4621 agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg tcttgaaatt
     4681 ccgaatatgt ccccgctgct gtgcgtgcgc acccttaacc accgtgacgg tgaaagcagc
     4741 ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac tatggagcac
     4801 tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc acacagccaa
     4861 ccggcagaac tggcagcccg cctgaacgcg ctgaacatca ccgccaacta tgaggtgatc
     4921 cgcgccgctg aaactggcct ggtacagatt caggcgcgga tgggcggcac cggcgaacgc
     4981 ttttttgccg gcgacgccac gctgacccgc gccgccgtac gtctgactga cggcaccctc
     5041 ggctacagct gggtgctggg gcgcgacaaa cagcacgccg aacgctgcgc gctgattgac
     5101 gcgctgatgc agcaaacccg ccattttcaa aacttatcag aaacgcttat tgccccgctg
     5161 gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc cagccgggtc
     5221 gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct tttatgcttc
     5281 ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc gagccgggcg
     5341 tgattgtcgc cctgcatcag ctcaaacgcg gctggcagcc gctgaatatc gccaccacca
     5401 gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccgcg ccattgagca
     5461 acgatatcgt cagccagagc ctgcgttttc ataccaacgc gccgctggtc agccagccgg
     5521 aacaggcgac cttcgcggta acggatgagg cgatttccag cgaacagctt aacgcccttt
     5581 ccaccggcac cgccgttgcg ccggaagcgg gcgcaacgct gattttacag gtcgccagcc
     5641 tgagcggcgg gcgcatgctg cgtctcaccg gcgcgggtat tgccgaagaa cgaatgatcg
     5701 ccccgcagct gccggagtgc attctgcacg aactcaccga gcgcccgcac ccgttcccgc
     5761 tcggcatcga cctgatcctg acctgcggcg agcgcctgct ggctgttccg cgaaccactc
     5821 atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gagaaggcga tcgacgccgc
     5881 ccacgccctg caagagagcc gacgccgggg cgataccgat ttgcctgaac tgagcgtcgc
     5941 ccagattgaa cagcagctta atcttgcggt ggatcgcgtg atgaccgaag gcggcattgc
     6001 cgaccgcgag ctggcggcgc tggcgctgaa acaggccagc ggcgataacg tcgaagcaat
     6061 tttcctgctg cgcgcctacc gcaccacgct ggcgaagctc gcggtaagcg agccgctcga
     6121 caccacggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata ttcccggcgg
     6181 ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca ccctgctggc
     6241 aaacggcgaa gcgccgacgc tgaccaccgc cgacagcgaa cagcagccgt cgccgcacgt
     6301 tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg gcgcacagcc
     6361 ggatgacatc acccgcacgc caccggttta cccctgctca cgttcttccc gcctgcaaca
     6421 gctcatgcgc ggcgacgaag gctatttgct ggcgctggcc tactccaccc aacgcggtta
     6481 cgggcgcaat cacccgttcg cgggtgaaat ccgcagtggt tacatcgacg tgtcgattgt
     6541 gcccgaagag ctgggatttg cggtgaacgt cggcgaactg ctgatgactg agtgtgaaat
     6601 ggtcaacggt tttatcgacc caccggatga atctccgcac ttcacgcgcg gctacgggct
     6661 ggtgttcggc atgagcgagc gcaaagcgat ggcgatggcg ctggtcgacc gcgctctgca
     6721 agccccggag tacggcgagc acgcgacagg cccggcgcag gatgaagagt tcgtgctggc
     6781 acatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc cgcactacgt
     6841 cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga accatggcta
     6901 atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg atccgccgcg
     6961 ccatcttaaa agcggtggcg atccccggtt atcaggtgcc gtttggcggg cgcgagatgc
     7021 caatgccgta cggctgggga accggcggca ttcagcttac cgccagcgtg attggcgaaa
     7081 gcgacgtgct gaaggtgatt gaccagggcg cggatgacac caccaacgcc gtgtcgattc
     7141 gcaacttctt taagcgcgtg accggggtaa acaccaccga gcgcacggac gatgcaacgg
     7201 tcatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag atcattattt
     7261 tccaggtgcc gatccccgaa ccgctgcgct ttatcgagcc gcgcgaaacg gaaacccgca
     7321 ccatgcacgc gctggaggaa tacggcgtga tgcaggtgaa gctgtatgaa gatatcgccc
     7381 gcttcggtca tatcgccacc acctacgcct atccggtgaa ggtgaacggg cgctacgtaa
     7441 tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg cccgccctgc
     7501 aactcttcgg cgccgggcgt gagaagcgca tctatgcggt gccgccgttt acccgcgtgg
     7561 aaagcctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag ccatgcgcca
     7621 tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc ggaaaccgca
     7681 tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcaaaa agccaatgaa
     7741 tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca aaggctttag
     7801 cgatgtctct tttgatttat ggccggggga agtgctgggc attgtcgggg aatccggctc
     7861 cgggaagacc acgctgctga aatcaatttc cgcgcgtctg acgccgcagc agggggagat
     7921 tcactatgaa aaccgttccc tctatggcat gagcgaggcc gaccgccgtc gcctgctgcg
     7981 caccgaatgg ggcgtagtgc atcagcatcc gctcgacggc ctgcgccgcc aggtctcggc
     8041 aggcggcaat atcggcgagc ggctgatggc gaccggggcg cgtcattacg gcgatattcg
     8101 cgccaccgcg cagaagtggc tggaagaggt ggaaattccc gccaaccgga tcgacgacct
     8161 gccgaccacc ttttccggcg gtatgcagca gcgtttgcag attgcccgca acctggtgac
     8221 gcatccgaag ctggtgttta tggatgaacc gaccggcggg ctggatgtgt cggtgcaggc
     8281 ccgcctgctc gacctgctgc gcggcctggt ggtggagctg aacctcgcgg tagtgattgt
     8341 cacccacgat ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga tgaagcaggg
     8401 gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcatc atccgtatac
     8461 acagctgctg gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg gcgtaaacgc
     8521 cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcatcag cgtcgcatcc
     8581 gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa aaccttcatc
     8641 ctgcaccagc aaaacggcgt gcgcttgccc gtcctcaacc gcgcctcgct caccgtcaac
     8701 gcgggcgaat gcgtggtgct ccacggccac tccggcagcg gcaaatcaac cctgctgcgc
     8761 tcgctgtacg ccaactatct gcccgatgaa gggcaaatcc agatcaaaca cggtgacgag
     8821 tgggtagacc tggtcactgc gccagcgcgc aaagtggtgg aaatccgcaa aaccaccgtt
     8881 ggctgggtga gccagtttct gcgcgttatc ccgcgcattt cggcgctgga agtagtaatg
     8941 cagccgctgc tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc cgcgcgtctg
     9001 ctcacccgtc tgaacgtgcc ggaacgcctg tggcgccttg cgccatcgac cttctccggc
     9061 ggcgaacagc agcgcgtcaa catcgcccgt ggctttatcg tcgactaccc cattctgctg
     9121 ctcgacgaac ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt cgaactgatc
     9181 cgagaagcca aagcccgcgg cgcagccatc gtcggcattt tccatgacga agccgtacgt
     9241 aatgacgtcg ccgaccgcct gcacccaatg ggagcctctt catgattatc aataacgtca
     9301 aactggtgct ggaaaacgaa gtggtaaacg gttcgctgga gatgcaggac ggcgaaatcc
     9361 gcgcctttgc cgaaagccag agccgcctgc cggaggcgat ggacggcgaa ggcggctggc
     9421 tgctgccggg gctgattgag ctgcatacag acaacctcga taaattcttc accccgcgcc
     9481 cgaaagttga ctggcccgcc cactcggcga tgagcagcca tgacgcgctg atggtggcga
     9541 gcggcatcac caccgtactg gacgccgtgg cgattggcga cgtgcgagac ggcggcgatc
     9601 ggctggagaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa cgcggcgtca
     9661 accgcgccga gcaccgcctg cacctgcgct gcgagctgcc gcataacacc acgctgccgc
     9721 tgtttgaaaa gctggtgcag cgcgagccgg tgacgctggt gtcgctgatg gatcactcgc
     9781 cgggccagcg ccagttcgcc aaccgcgaga agtatcgcga atattatcag ggcaaatact
     9841 cgctcaccga tgcgcagatg cagcagtatg aagaggaaca gctggcgctt gccgcacgct
     9901 ggtcgcagcc gaatcgcgaa tccatcgccg caatgtgccg cgcgcgacaa attgcgctcg
     9961 ccagccacga tgacgcaacg cacgcccacg ttgccgaatc tcaccagctt ggcagcgtga
    10021 tcgccgaatt tcccaccacg tttgaagcag cggaagcctc gcgcaagcat ggcatgaacg
    10081 tgctgatggg cgcgccaaat atcgtgcgtg gcggttcgca ctccggcaac gtggcggcca
    10141 gtgaactggc acagcttggc ctgctggata tcctctcttc cgactactac cccgccagcc
    10201 tgctggatgc ggcgttccgc gtcgccgatg accagagcaa ccgctttacg ctgccgcagg
    10261 cggtgaagct ggtgaccaaa aatccggcgc aggcgcttaa tctccaggat cgcggggtga
    10321 ttggcgaggg caaacgcgcc gacctggtgc tggcgcatcg caagggcaat cacattcata
    10381 tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac tgatttggtt
    10441 aatggggccg tccggctccg ggaaagatag cctgctggcg gaactccgcc tgcgggaaca
    10501 aactcagcta ctggtggcgc atcgctacat cacgcgcgac gccagcgccg gaagcgaaaa
    10561 ccatatcgcc ctgagcgagc aggaattttt tacccgcgcg gggcaaaatc tgctggcctt
    10621 aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagattgacc tctggctgca
    10681 cgctggattc gacgtgctgg tcaacggctc acgcgcccat ctgccgcagg cgcgggcgcg
    10741 ctatcaatcg gcgctgctgc ccatttgctt acaggtttcg ccagagatcc tgcgccagcg
    10801 cctggaaaac cgtggccgcg aaaacgccag tgaaattaac gcccggctgg cgcgcgccgc
    10861 ccgctatact ccacaggatt gccatacgct caataatgac ggcagcctgc gccagtcggt
    10921 cgacacgctg ctgacgctaa tccatcagaa ggagaaacac catgcctgct tgtgagcttt
    10981 cgcccggcca cgcagtacga caccgacgcg gtttacgcgt tgatttgcga gctaaaacag
    11041 gcggagtttg accaccacgc gtttcgcgtg ggctttaacg ccaacctgcg cgacccgaac
    11101 atgcgctacc atctggcgct gctggatggc gaagttgtcg gtatgatcgg cctgcatttg
    11161 cagtttcatc tgcatcatgt caactggatc ggcgaaattc aggagttggt ggtaatgccg
    11221 caggcgcgcg gtctgaacgt cggcagtaag ttactggcgt gggcagaaga agaagcccgc
    11281 caggccgggg ccgaaatgac cgaactttcg accaacgtga agcgccacga cgcgcaccgt
    11341 ttctatctgc gcgaaggcta cgagcagagc cacttccgct tcaccaaggt gctgtaacat
    11401 gagcctgacc ctcacgctca ccggctccgg cggcgcacag ggcgttccgg catggggctg
    11461 cgagtgtgcg gcctgcgcca gagcgcggcg ctcgccgcac tatcgccgcc agccatgcag
    11521 cggcgtagtg aagtttaacg aggcgatcac cctgatcgac gccgggcggc acgatctcgc
    11581 cgatcgctgg tcgcccggat cgttccagca gtttttgctg acgcactacc atatggatca
    11641 cgtgcagggg ctattcccgc tgcgctgggg cgtgggcgat ccgatcccgg tttacggccc
    11701 accggatgaa cagggctgcg acgatctgtt taaacatccg ggcctgcttg atttcagcca
    11761 cacggtggaa ccgtttgtgg tgtttgattt gcaggggtta caggtcacac ccctgccgct
    11821 caaccactca aaactgacct tcggttatct gctggaaacg gcgcacagcc gggtggcgtg
    11881 gctgtctgac accgctggct tgccggaaaa aacgctgaaa tttttacgca ataatcagcc
    11941 gcaggtaatg gtgattgatt gcagccaccc gccgcgcgag gatgcaccgc gtaatcactg
    12001 tgatttaaat accgtgcttg cgctgaatca ggttatccgc tcgccgcgcg tggttctgac
    12061 ccatatcagc caccagtttg atgcgtggct gatggaaaac gcactgccgt cggggtttga
    12121 ggcggggttt gatgggatgg agattggggt ggcgtgatga gagggaatgt gcgtgctggc
    12181 cccctcaccc taaccctctc cccagagggg cgatgcctgc cccataccca acatcattgc
    12241 ccatactcat cttccattct ccgctcttca tcttctagct gccgacgctc ctgattaagc
    12301 tggtgctggc gatcgtccag ctgcctgcga cgatcttcaa actggcgacg gcggtcgtca
    12361 tattgtctgc gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca
    12421 taatctcgcc cacggtcagg gttagaagcg tcgttaatcg cctgctgaat attgccaatg
    12481 gtgtcatcga taatatcggc ctgggccggt acggtggtca gcgtgagcag ggtgaaaaga
    12541 aaaagcgtgg agtggcgttt cataaactcg gcctcgcaac aaagttaata caaggtaggc
    12601 aaaggtgcct gttacgagta gaggaggatt ctcaattctc ttgccccagc gcctgcgcca
    12661 actgcgcacg ggtaaagggt ttgcgcaaca gcgccacgtc tggcagcgcg gggttatggc
    12721 tggggcgtaa atcctgaccg ctgatgagta gcagcgtcag gtgcgggtaa agtttgcggg
    12781 ccgcattgac cacttctgcg ccgctcatgc cgccggggag cattaaatcg ctgataaaaa
    12841 tatcgatttc tgctgatgcc gccagcagat ccagcgcctg ttcgccgctg cttgcttcca
    12901 gcgtcaaata gcccagcagg tgcaactgtt cgcagatggt ctggcgcacc gcggcttcat
    12961 cttccagcac taacaccagt ttatcgccgc tgacagaagc ctgttctact gccgctggcg
    13021 ataggttttt cgccgccgtc agcgcgcggg gaagctgtaa tctgaccgtt gtcccctgcc
    13081 ccggcgcgct ttcaatttca acgcgcccac cggactggcg cacaaaacca tagaccatcg
    13141 acaaacccag cccgctgccg ctgcccgtct gtttggtggt aaagaatggc tcaaatacct
    13201 ggcttttcac cgcctgcgac ataccgcagc catgatcgat gacctccagt gccaccatgt
    13261 cctgacgctg cccactgctg cgagtaacgc gctgattcca ggtacgaatt ttgatgattc
    13321 ccgtctgccc ttccatcgcg tcacgggcgt tcatcaccag gttgatgatg gcattttcca
    13381 gttggctgat gtcaatccac gcgggccacg ccggagactg cgcttcaatc tccagcgcca
    13441 gcgttgccgg taaggagtgg cgcattaatt cgcttaactc ttccagcaat gttttcagtt
    13501 caacggcttg cggatgtaag gactgcttgc gggaaaacgc cagcagtcgt tgtgtcagta
    13561 gcgccccacg ttcggcagct tgcagcgccc gctgtattcg cggcgcgtct ggcgagtccg
    13621 gattcaccag ctccaggctg ccaataatca ccgccagcag attattaaaa tcatgggcca
    13681 gcccgcccgt cagttgcccg acagctttca gtttctggct gtgtaatagc gcctcttcca
    13741 acccctgccg ctcagtttgg gcaatctcca tctgcgtggt cttctctttt agcagccgtg
    13801 aggttcgttc gagtgacgcg gtattgcggg caaaaacgtt aaaggcgcgc gccagttcac
    13861 ccaactcatc acgccgctgt aacgccggaa aagtgacgtc cgcttcgccg tgcgccaggc
    13921 ggctcatggc gcgggaaatc gccgtcaggt tagtgccgag gttgcgatag atataccagc
    13981 cggcaaacgc ggtgatcacc accgctaaca gggcgaaaat catgatcacc ctctcgccgg
    14041 agcgcaaatc ctggtaactt tgcgccacac gctgaccgga gagcgtcgcc acctgttcgg
    14101 cgtaacggtt gatgtcgcca ttgaggatcg ccaccagcgc tttgatgtga aacatatacc
    14161 agctgattgc cagatcgctt tgctccagag cggcagaaag cggcgcgagt ttacgcattt
    14221 catcattgaa atcattgaaa tcattgagta acgtattaac caatggcgta tcgctgtggg
    14281 tcggcaacgc agacattacc ccgaccagtt gatgaataat cgcctgcggc gcgggggtgg
    14341 caatagcggc aacaatcagt cggttcatct cgttaagcaa aatatcatct tgcgcgtgag
    14401 tgactttttg cagatgccgc agataactta agttttgata cagcgaactt aacagcgcgt
    14461 tgcgctccag atgccgcctt tgcccgcgct ccagcatacc gccgacgctg gtttgcagtt
    14521 cgttgctacg cgtgatgatt cgcgtcacca gcccggcttc ctgctgcgct aaaggcgcgt
    14581 tcgccagttg tgcaagcgag ctttttaatg cccgctgtgt ctcctgcaac cgctcggctt
    14641 cgcctttata ttccagcgct cccaccacct gcgacaatcg caccgccgcc gttgccacgt
    14701 ttgccgtgtc gcgcgccaga ttcaggctgc cggtcatatc atccaccgtt tgctgctgca
    14761 ccagctcctg catctggctg gcatgacgaa agcccattac cgccacgccg ctgaccatca
    14821 acgtcaccgc caccaccagc aaattcaaaa gcagcaatcg cccgcgcgcg ctggcaaaaa
    14881 agtgacgtcc ggggtgcgcc atatcacgct ccgtgaagaa aaatcgcaat atgacaaata
    14941 tgaacggctt ctgacatttt gcatttactt tcctgtgatg gagtgctgat atcgacaacc
    15001 tcgcaggagt ccaccatgaa cagcacgcca gttctggaga tgcgcaatat tgccaaagcc
    15061 ttcggcaaat tttatgcact caaaggggtg gatttgacgg tctaccctgg cgagatccat
    15121 gccctgatgg gggaaaacgg cgcgggaaaa agcacgctga tgaaggtgct ggcgggggcg
    15181 tataccgcca ccagcggcga gattctcata gacggcaagc cctttcacat tcgcacgcca
    15241 aaagatgcct taagcgccgg tattacgctg atttatcagg agatgcagct ggcaccgaat
    15301 ctgtcggtgg cagaaaatat ttttctcggc agcgagcttt cccacggcgg gctggtgcag
    15361 cgtaaagaga tgctagtgca ggcgcaaaaa gtgatcgacc gcctcggcgc gcagtttaac
    15421 gccagcgata aggtcatgac gctgaccatt gccgagcaac agcaggtcga aatcgcccgc
    15481 gcactacatc gcaacagccg cattctggtg atggacgaac ccaccgctgc cctctcctcc
    15541 cgcgaaactc accgcctgtt tgaactgatt atgcggctgc gcgatgaagg gatggcgatt
    15601 atctacatta gccaccgcat ggcggaagtg tatgagcttt ccgatcgcgt cagcgtgcta
    15661 cgcgacgggc aatacgttgg cagcctgacc cgcgataacc tcaatgccgg ggagctggtg
    15721 cggatgatgg tcggcaggcc actgagcgat ctgttcaata aagagcgcga tatcccgctc
    15781 ggtaaagccc gcctgaatgt tcaccacctg accgacggcg gcaaagtcca gccgagtagc
    15841 ctgctggtgc gttccggcga aattgttggc ctcgccggac tggtgggtgc cggacgttcc
    15901 gaactggcgc agttgatctt cggcgtgcgg aaagcgacag gcggaatgat tgaagtcgat
    15961 ggtgaaccgg tggtgatcca ctccccgcgc gaagccatcg atcttggcat tggttttctc
    16021 accgaaaacc gcaaagaaca aggcttattc cttgaaatgg cagcagccga aaacatcacc
    16081 atggcaaccc tggagcgcga tgcccgctgg ggaatgctca atcgcaaaaa agcgcaaacc
    16141 atttccgatg acgccattaa gttgctcaac attcgcgtgc ctcatgccca ggtacgcgcg
    16201 ggcgggcttt ccggcggcaa tcagcaaaaa ctgttgatct cccgctgggt ggcgattggc
    16261 ccgcgcattt tactgctcga tgaacccgcc cgcggcgtgg acgttggcgc caaaagtgag
    16321 atctaccgga tcatgaacga gatggcgcgc aagggcgtgg cgatcctgat gatctccagc
    16381 gaactgccgg agatagtggg aatgagcgat cgcgtctatg tgatgcgcga aggcagcatt
    16441 gccggtgagt taaacggcaa aaacatcacc caggaaaaca ttatgacctt agcgactggc
    16501 gtcaacgacg cccattccca ggcggtaacc tcatgacgac ctctgcaaaa acctctgcca
    16561 aaaaaatgct gatgagcgat ctgatgcaaa cggtcggcat cttgcccatt ttgattctga
    16621 tcgtggcggt atttggcttt atcacgccaa acttttttac cgaaagcaac ctgctgaata
    16681 ttacccgcca ggcatcgatc aacattgttc tggcggcggg gatgaccttc atcattttga
    16741 ccggcgggat cgatctttcc gtgggttcga tcctcggcac cacagcggtg gcggcgatgg
    16801 tcgtctccct tagccctgag ttcgccatgc tgtcgatccc ggcagcgctg atgctcggcc
    16861 tgctgctggg gctgttcaac ggcgcgctgg tggcctttgc cggattgcca ccgtttatcg
    16921 ttacgcttgg cacctatacg gcgctgcgcg gggcggcgta tctgctggcg gacggcacca
    16981 cggttatcaa ctccaacatt aatttcgagt ggataggcaa caactatctc gggccggtcc
    17041 catggctggt ggtgattgcg ctggcggtga tcgtggtgtg ctggtttatt ctgcgccgca
    17101 ccacgcttgg cgttcatatc tacgccgtcg gcagcaatat gcaggcggcg cgtcttaccg
    17161 gcattaaggt ctggctggtg ctgctgtttg tttatggcat gagcggcctg ctttctgggc
    17221 ttggtgggat tatgagcgcc tcacggctgt acagcgccaa cggcaactta ggcatgggtt
    17281 acgaactgga tgccattgca gcggtgatcc tcggcggcac cagttttgtc ggcggcatcg
    17341 gcaccatcac cggcacgctg gtcggcgcgc taattatcgc cacgctcaac aatggcatga
    17401 ccttaatggg cgtctcttac ttctggcaac tggtgatcaa aggggcgatg atcatcattg
    17461 cggtgctgat cgacaaatac cgtacccgac accatcaaag tgcataaaca catgaggaat
    17521 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    17581 caccgttcgc ccaggcgaaa gaactgaaat caataggcgt aacggtgggc gatctggcga
    17641 acccgttctt cgtgcagatc accaaaggcg cggagctgga agcacgcaaa ctggcaggcg
    17701 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtgtcgcaga
    17761 tagacaactt tatcgccgct aacgtcgata tgattatcct caacgctgcc gattcaaaag
    17821 ggatcggccc ggcggtgaaa cgggcgaaag atgccgggat cgtggtcgtc gcggttgacg
    17881 tagcggcaga aggtgccgat gccaccatca cttccgataa cacccaggca ggcgaaatgg
    17941 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggcc
    18001 cgccggtatc cgccgtgcaa aaccgcgtcg aaggatgcca gactgaattt aaacgccatc
    18061 ctgacatcaa agtgctctct gacaaccaga acgcgaaagg cagtcgtgaa ggcgggctgg
    18121 aggtaatgac gggactgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    18181 atcccaccgc aatcggtgcc gatctggcag caaaacaggc acaacgtaac gagttcttta
    18241 ttgtcggtgt ggacggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    18301 ttgtcgctac tccggcgcaa gatccccagg tgatggcagc gaaagcggtg gaaattggct
    18361 acgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    18421 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    18481 gcggcgcagc tgcccggtca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    18541 acgacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    18601 ttgccagttt ctcgcctgct caatggcagc agctcgacac cgccgcgtgg gaggaggttt
    18661 ttgatcttaa actcggctgg gatgttaccg cttttggtcg caacaacttt gcagcacacg
    18721 ggctgacgct gtttacccta cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    18781 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    18841 gtaagcgtga ggacattatc aatcgcggcg gcggtaattt aattgttgaa ctgtggaatg
    18901 ccgatagtaa cgagcaaacg gctgatagtg acattacggt ggtgatagac ggctgccgcc
    18961 agaaacatac tgcaggctct cagttgcgcc tgtctcccgg cgaaagtatc tgcctgccgc
    19021 ccggcctgta tcacagcttt tgggcagaag ccggttttgg cgatgtgctg gttggcgaag
    19081 tctcttctgt taatgacgac gaccacgata accacttttt acagccactg gaccgctaca
    19141 acctgattga cgaagacgaa cctgcacagt tggtattgtg taacgaatac cgccagttcc
    19201 gctgaataag gataacgtta tgccattgat ttctctcgct gaagggctgg cacatgcccg
    19261 cgaacatcac tattcgttag gtgcgtttaa tgtgctggac tcgcactttt tacgcgcctt
    19321 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    19381 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcatga
    19441 cattccggtg gtgttaaatc tcgatcacgg actgcatttt gaatcggtgg tgcaagcgtt
    19501 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagaaaatgt
    19561 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    19621 actgggcgcg gtgggtggcg acgaaggcgg tgcactctat ggtcatgccg atgaatccct
    19681 gtttaccgat ccgcaactgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    19741 cgtggcgatt ggcaatgcac acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    19801 cctcgacgct atccgccagc aggctgctat cccgctggta ttacacggcg gctcagggat
    19861 tagcgatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    19921 tggcatgtcg caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    19981 gtatgacgag tttgccgagt tactgctggc agtggaagcc gccatcagcg atacggttgc
    20041 acaacagatg cgtatttttg gcagtgcggg gcaggcatga tggagcgtaa agggattatc
    20101 gccgcaggca atatgttggt tgaccacgtc catcagatta tccagtggcc agagcgcggc
    20161 tggctggcgg agatcaccca cagcgaacgc tccaccggcg gtgcgccgct taacgtcctg
    20221 ctgacgctgg cgaaaatgcg caccgggctg ccgttacagg cggttgggct aatcggtcag
    20281 gacagcgacg gcgattacat catggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    20341 gtgcagcgca ctacctttgc cccgacgtcg atgtcgcagg tgatgacaga cccaagcggg
    20401 cagcggacgt ttttccattc gccgggtgca aacagacttt tggatcttcc ggcgttcgat
    20461 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    20521 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    20581 actggctttg aaacctcact cgacctggtc tcccgtaagg gcgatccgcg ttatcagccg
    20641 ctggtgctgc ccgcgttgcg ccatctggat tatctggtaa ttaacgaact ggaggccggg
    20701 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcac gcatattgct
    20761 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gggtggtgat ccactacccg
    20821 gaaggggcgt ggggagagac cccagaacag ggcggcgtgt ggatcccttc gcaaaagtta
    20881 gaacaacggg aaattatcgg cagcgtcggc gcgggcgatg cattttgtgc cggattttta
    20941 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    21001 cgggcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    21061 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    21121 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    21181 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctgt gggtgaacgc cagcaactgc
    21241 tcgcggctca atacccgacg cgcgttttgc gccagagcca gtaacaagcc atattcgccc
    21301 tgagttaact cgacatgttg ctgaagcgga ttgtgcaact ggcagcgcat ggtatcgaga
    21361 taccagccat tgaatgacag cccggcgctt ttctcgtcat taaccgccag cgcgccaacc
    21421 cgtcgcaaca ccgctttcag tcgtgcgacc accacccgcg gggtgaacgg tttgcagatg
    21481 taatcatccg cgcccatttc cagcccgacc accacatcag actcactgcc catgccggtc
    21541 aacatcacca ccggcaatgt cggacgcagt ttctggattt gctgcaatac cctcaggccg
    21601 ttggtatcgg gtagcatcat atccagcacc accagcgcaa tgtcaggatc gccctcaata
    21661 cgcaatatgg cctcctgtcc ggtatggcag actgaaaccg taaagacatg ttcacttaac
    21721 acgtcctgca acagtgcgca gatagccgtg tcatcatcca ccaccagcac gacgggtttc
    21781 atggttacac tccgcatcat tcatctgatt aatcattgtg cgtacttctt gtcgaagtga
//
LOCUS       DABSYT020000077        21367 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.077, whole genome shotgun sequence.
ACCESSION   DABSYT020000077 DABSYT020000000
VERSION     DABSYT020000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.077"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            204..704
                     /gene="tssB"
                     /locus_tag="ID725_RS17040"
                     /old_locus_tag="pgaptmp_003408"
     CDS             204..704
                     /gene="tssB"
                     /locus_tag="ID725_RS17040"
                     /old_locus_tag="pgaptmp_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGV7851927.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            739..963
                     /locus_tag="ID725_RS17045"
                     /old_locus_tag="pgaptmp_003409"
     CDS             739..963
                     /locus_tag="ID725_RS17045"
                     /old_locus_tag="pgaptmp_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851928.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1014..2405
                     /gene="tssC"
                     /locus_tag="ID725_RS17050"
                     /old_locus_tag="pgaptmp_003410"
     CDS             1014..2405
                     /gene="tssC"
                     /locus_tag="ID725_RS17050"
                     /old_locus_tag="pgaptmp_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGV7851929.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2496..2909
                     /gene="tssE"
                     /locus_tag="ID725_RS17055"
                     /old_locus_tag="pgaptmp_003411"
     CDS             2496..2909
                     /gene="tssE"
                     /locus_tag="ID725_RS17055"
                     /old_locus_tag="pgaptmp_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGV7851930.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2913..4763
                     /gene="tssF"
                     /locus_tag="ID725_RS17060"
                     /old_locus_tag="pgaptmp_003412"
     CDS             2913..4763
                     /gene="tssF"
                     /locus_tag="ID725_RS17060"
                     /old_locus_tag="pgaptmp_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGV7851931.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4727..5809
                     /gene="tssG"
                     /locus_tag="ID725_RS17065"
                     /old_locus_tag="pgaptmp_003413"
     CDS             4727..5809
                     /gene="tssG"
                     /locus_tag="ID725_RS17065"
                     /old_locus_tag="pgaptmp_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGV7851932.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5834..7114
                     /gene="tagH"
                     /locus_tag="ID725_RS17070"
                     /old_locus_tag="pgaptmp_003414"
     CDS             5834..7114
                     /gene="tagH"
                     /locus_tag="ID725_RS17070"
                     /old_locus_tag="pgaptmp_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HGV7851933.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7111..7635
                     /gene="tssJ"
                     /locus_tag="ID725_RS17075"
                     /old_locus_tag="pgaptmp_003415"
     CDS             7111..7635
                     /gene="tssJ"
                     /locus_tag="ID725_RS17075"
                     /old_locus_tag="pgaptmp_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGV7851934.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7638..8969
                     /gene="tssK"
                     /locus_tag="ID725_RS17080"
                     /old_locus_tag="pgaptmp_003416"
     CDS             7638..8969
                     /gene="tssK"
                     /locus_tag="ID725_RS17080"
                     /old_locus_tag="pgaptmp_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGV7851935.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8974..9735
                     /gene="icmH"
                     /locus_tag="ID725_RS17085"
                     /old_locus_tag="pgaptmp_003417"
     CDS             8974..9735
                     /gene="icmH"
                     /locus_tag="ID725_RS17085"
                     /old_locus_tag="pgaptmp_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGV7851936.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9744..12515
                     /gene="tssH"
                     /locus_tag="ID725_RS17090"
                     /old_locus_tag="pgaptmp_003418"
                     /gene_synonym="clpV"
     CDS             9744..12515
                     /gene="tssH"
                     /locus_tag="ID725_RS17090"
                     /old_locus_tag="pgaptmp_003418"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGV7851937.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLTAE
                     LDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQD
                     LAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTI
                     NMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLF
                     YQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFK
                     PALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVT
                     RAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDE
                     SVTKDETVL"
     gene            12512..13255
                     /gene="vasI"
                     /locus_tag="ID725_RS17095"
                     /old_locus_tag="pgaptmp_003419"
     CDS             12512..13255
                     /gene="vasI"
                     /locus_tag="ID725_RS17095"
                     /old_locus_tag="pgaptmp_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGV7851938.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13260..14672
                     /gene="tssA"
                     /locus_tag="ID725_RS17100"
                     /old_locus_tag="pgaptmp_003420"
     CDS             13260..14672
                     /gene="tssA"
                     /locus_tag="ID725_RS17100"
                     /old_locus_tag="pgaptmp_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGV7851939.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHAQLDIPELQRRGLQLLASESKDFRLLAHLLRTLQHAGDPLLALHLLTLYVEH
                     YWTVAAPQNMAHKKRFASQIIKRFETGIEGFSQNAATTQRDTLLGELAKLAQCWQSHN
                     VPELAQATDDLFALYQRTFNRAAPAPVPTPAASGSSPQTTVTSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14809..18243
                     /gene="tssM"
                     /locus_tag="ID725_RS17105"
                     /old_locus_tag="pgaptmp_003421"
     CDS             14809..18243
                     /gene="tssM"
                     /locus_tag="ID725_RS17105"
                     /old_locus_tag="pgaptmp_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGV7851940.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18254..19663
                     /locus_tag="ID725_RS17110"
                     /old_locus_tag="pgaptmp_003422"
     CDS             18254..19663
                     /locus_tag="ID725_RS17110"
                     /old_locus_tag="pgaptmp_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HGV7851941.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            19629..20108
                     /locus_tag="ID725_RS17115"
                     /old_locus_tag="pgaptmp_003423"
     CDS             19629..20108
                     /locus_tag="ID725_RS17115"
                     /old_locus_tag="pgaptmp_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGV7851942.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELFYK
                     IKLTGAKISDIHVSISHIVVDNSVQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
     gene            20129..20351
                     /locus_tag="ID725_RS17120"
                     /old_locus_tag="pgaptmp_003424"
                     /pseudo
     CDS             20129..20351
                     /locus_tag="ID725_RS17120"
                     /old_locus_tag="pgaptmp_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20430..21026
                     /locus_tag="ID725_RS17125"
                     /old_locus_tag="pgaptmp_003425"
     CDS             20430..21026
                     /locus_tag="ID725_RS17125"
                     /old_locus_tag="pgaptmp_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HGV7851943.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITIKNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            21029..>21367
                     /locus_tag="ID725_RS17130"
                     /old_locus_tag="pgaptmp_003426"
     CDS             21029..>21367
                     /locus_tag="ID725_RS17130"
                     /old_locus_tag="pgaptmp_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851944.1"
                     /translation="MRKITIILLLTFSCTKGLAFDISMSKECSGVDELEFIDCVKNSF
                     IMSTITLKKAEANIKEKIQKWNSDEDEAIRKEAVISLKRLESSNREFIRYRFAQCSFT
                     LTWGARVGRLA"
BASE COUNT         5240 a         5714 c         5760 g         4653 t
ORIGIN      
        1 aaatctattt aacgcttgca gaatacccct ctttacgatc gataaaaagt ccttttctta
       61 tttattttga agcgtaaaat gcggggtgat gaaggagtgg tgattttatt ttcaccgcca
      121 tcaagacggc atttccgata ttttttgcgg tacgaatatg ccgtgttcat ggcctgtacg
      181 agataacatg acggatcact tttatgagca aaaaatttga aggcagcgtc gcgcctcgcg
      241 aacgtattaa tatatcgtat gtcccaaaaa cagatggcca gacggctgaa gtggaacttc
      301 cgcttaacat gctcgttgtc ggcgataccg gaaatacgca ggaaacatcc tcactggatg
      361 aacgtcaggc agtttccgtt aacaagcaca atttcggtgc agtgatggct gaagcagcca
      421 ttggcctcaa ttttaccgtc cccgccacgc tgaagggaag cacaacggac gatgaaatga
      481 atgtggcgct gaatatcaag tcactggatg atttttcacc agatagcgtg gcccggcagg
      541 ttccggaagt gaataaattg ctggaactgc gtgaagcact gactgcactg aaggggccga
      601 tgggtaacct tccggcattc cgtacccagc tccaggcatt gctggaaaat gaagagtccc
      661 gcgagcagtt gctcaaagaa atcggccagg tgagcaataa ataaccacca ctatttcatt
      721 aaacagggtt atccaggcat gacggcacac aaaaatatct ccgggacata ccacctctct
      781 gttgcagaca ttctgcaggt ggtttaccag gtatgtttca gcccgtccgt tgaaatcaac
      841 caggatggtg tggctgcgct gattacaaca ctggatcgcc gtatcagtga tctattggat
      901 gagattatcc atttttgtga gtttcagcag agcgcgtcgc actggcagcg cgtacttcac
      961 tgacggcgaa aagccggttt cagaaacaca gctaaaacag aaggattttc agtatgtctt
     1021 tacaggaaga ggaactcgtt tccagtcatg ccgggcagcc tgagcaggaa tcgtcgctgc
     1081 ttgatcaaat catggcacag acccgtattc agcccggttc ggaaggttat gacgttgccc
     1141 gccagggtgt gacagccttt attgccagca tcctgcaatc cactgcatct gccgaaccgg
     1201 tcaataaact ggccgtggac agcatgattg cagatattga tgaacgcatc agccgccaga
     1261 tggatgtcat catccatgcg cctgcctttc agcaggtcga gtcgttctgg cgctcactga
     1321 aaaccatggt ggatcgcgtt gatttccgtg aaaacatcaa ggtcaacgtg cttcatgtca
     1381 ccaagcagga acttctggaa gactttgagt ttgcgccaga aattattcag tccggatttt
     1441 ataagcacgt ttattcgtcc ggtttcggac agtttggcgg tgaacccatc gctgccgtgc
     1501 tgggcgctta tgagttcaaa aataccgcgc ctgacatgaa actgctgcaa tacgtcagtg
     1561 ccgtgggcgc aatggcgcac gcaccgttcc tgtcctccgt ttccccggag tttatggggc
     1621 tgaactcgtg gaccgaactg cctaatatca aagatcttta tgccatcttt gaaggtccgg
     1681 cctacaccaa atggcgcgcc ctgcgtgact cggaagattc gcgctattta gggcttacgg
     1741 caccccgctt cctgcttcgc cagccctatt caccaacaga taaccctgtt aagaacttta
     1801 actattacga agatgtcagc cagaaccacg aagattatct gtggggtaat acggcctgga
     1861 tgctggcatg caatatcgca gacagttttg ccaaataccg ctggtgtcca aacattatcg
     1921 gtccgcaaag cggcggcgca gtgaaagatc tgccggtgca tctgttcgaa acgatggggc
     1981 agattcaggc caaaatccca accgaggtgc tggtcaccga tcgccgcgaa tttgaactgg
     2041 ctgaagaggg ttttatcacc cttaccatgc gtaaagactc tgataatgca gcctttttct
     2101 ctgcaaactc ggtacaaaaa ccgaagcact ttccgggaaa agatgcagaa accaactata
     2161 agctgggtac gcagcttcct tatctcttca tcatcaatcg gttagcgcac tacatcaagg
     2221 tgttgcagcg tgaacaactg gggtcatgga aagaaaggag cgacttagaa agagagctta
     2281 atacctggat ccgtcagtat gttgccgacc aggaaaaccc gcctgcagac gtgcgcagcc
     2341 gcaaacctct gcgcgctgcc aaagttgagg ttatggatgt agaaggcgaa cccggctggt
     2401 actaggttgc gctaagcgtg agacctcatt ttaaatttat gggggcaaat tttgagcttt
     2461 ccctggttgg ccggttagac agggagtaac acaccatgat actggatcgg gaaagaacca
     2521 caggttctct ttttgagcgt atggaagcct cttcggccag aaaccggcag ggaggcagta
     2581 ttcattcgct caggcaatcc attcgccaga acctaagaaa cattcttaat acccggtccg
     2641 gtagttgccg gggtgcgcct gagctgggca ttgatgaacc ggaaggggct gaaaacttcc
     2701 gcgaatccat gagtcgggcc atcgagcaat gtatagaacg ctatgaaccc cggatctcac
     2761 acgctgaagt gcaggcagtg gtttcatccg cctccagccc gctggatatg acgtttcata
     2821 ttacagcctg ggtgacattt aacgaaacgc atgaggtgct tgaatttgat atggcaccga
     2881 atggcagcca gcattaccgg gtggactgac aaatggaatt tgaagaacgc tattttcggg
     2941 aagaactcga ttacctgcgc cagcttagca agctgctggc aacggaaaaa ccccatctgg
     3001 cccgcttcct ggccgaaaaa gatgcggatc cggatattga acgcctgctg gaaggggtgg
     3061 cttttcttac cggcaatctc cgccagaaaa ttgaggatga attcccagaa ctgacgcacg
     3121 ggcttattaa gatgctatgg cctaattacc tgcgtccggt tccggcaatg acccttattg
     3181 aatatacgcc ggatatggat aagtcttctg taccggtgtt aatcccccgt aatgagcagt
     3241 ttacaaccaa cgccggggaa atcagagttg atgaagtgct gccctctgat gctaaaaagg
     3301 aggagccgcc tccctgtacc ttcaccctct gccgggatat ctggctgctg cccgttcgcc
     3361 tggagcagat tgaaaaccgc agtacgaccc gtaatggtgt tatcaacatc accttttcgg
     3421 tcgcaccggg aacggacttc cgcacgctgg atctgaacaa acttcgcttc tggctcggca
     3481 atgacgacaa ctatacccgt gaccagcttt atttatggtt ctgcgaatac ttgcagggtg
     3541 ccgacctgac tgtgggtgaa cagcatattc gcctgcctga gtttatgcta aaagctgtcg
     3601 gttttgagcc gcaggatgcc atgctgccct ggccgaaaaa cgtccacagc ggctaccgga
     3661 tccttcagga gtatttctgt taccccgatg cgtttctctt ttttgatctt tgtggttgtc
     3721 cggctttgcc tgacggattg caggcggaat tctttaccct gcaactgcgt ttttcgcgcc
     3781 ctttgcccgt ggacatccgg ctgcgccgcg attccctgcg cctgtattgc gcacctgcca
     3841 ttaatttatt tatccaccat gcagaagcca tcacgctgga caaccggcgg gcagactatc
     3901 cgctggttcc cagccgccat tacccacaac attacgatgt attttccgtt aacagtgtgg
     3961 tgagccaggt ccaggatatg ttcaggaaaa aagatctggg gcgtcctgtt tcgacgcagg
     4021 ccgcgcgcca gtggccagcc tttgaaagtt tcagccatca gatggaatac agccggaagc
     4081 gggaggtggt gtactggcat caccggacca aaacatccct gttccatcgc ggctttgatc
     4141 atacccttgc ctttatacat gctgatggca gttatccgtc agacgaatct ctgctcagta
     4201 atgaagtggt ttcggtatcg ctgacctgta ccaaccgtga gcttccgtca caaattcgtt
     4261 ccggcgatat caccggcaca accggtaaaa atgcggctgt tgcttccttt cgcaacatta
     4321 cccgcccgac gcaaccactc tggccggtca ttgatggcag cctgcactgg tccctgctct
     4381 ccgccatgaa cctgaattat ctgtcattac tggatacgga cgcgctgaag caggtcatcg
     4441 ccaactttga tcgccacgca atccaccatc cgcagacggc gcggctgtca caacaaaagc
     4501 tggatgccat tgagcgtctg gagacccgcc ccgttgatcg cctgtttacg ggtattcccg
     4561 tccggggact ggcctccacg ctgtatctgc acccggagcc gtttgtctgt gaaggggaaa
     4621 tgtatctgct cggtacggtg ctttcgcatt ttctgtcgct gtacgccagc gttaactcat
     4681 tccacatgct gaccgttgtg aacacagaaa gccaggagac atggaaatgg acggaaagaa
     4741 tcgggcagca tcctcttatc tgagtccggg aaatcccccg gcagataaag agcagaatga
     4801 tccgctggca caggttttcc ataatgcctg ctcttacaat ttttttgcga tggcggagct
     4861 gttgcaccgc ctggcaaagg gtgaaaaggg gacgccagaa ttatccctgc gcgacgatcc
     4921 ggcacaggaa accctgcgtt tcagcgccga tgccagcctt gccttcccgt gcagtgatat
     4981 cagtgcgctg aaacgggata cgtcaggggc attcaggatg accactacct ttatggggtt
     5041 acaggggagc cagtcgcctc tccccggtta ttacctcgat cacctggcct ggaaagcggt
     5101 acatgaacaa agtccggtag gtgattttct ggatatgttc agccaccgcc tgacccagtt
     5161 tgtctggcat atctggcgta agtaccgcta tcacatcagt tttcgtaacg gcggtgtgga
     5221 tgcgttctcg cagcgcatgt actcccttgt cgggctgggc caccgccagc ttcgcgataa
     5281 gctggcgatt aatcacagca aaatgctggc gtattccggg atcctggcaa atccgggacg
     5341 atccccggaa attatctgtg gcctggtgtc ccactgcttt gatttgtcag aagtgacgct
     5401 gcaaaactgg caacgtcgca aggtggatat tgaaccggat cagcaaaaca gcctggggag
     5461 ctattctctt aaaaatggcg aaaaactggc gggaagatcg gtactgggca actttgttct
     5521 gggtacacga gtacccgatc tcagcggaaa attccagctc agtattacca gcctgacccg
     5581 aaaacagttc ctctcttttc tgccgtccgg cgaaaacttc ctgccactga cgacgttcgt
     5641 gtccttcatc ctgcgcgatc agcttgcctg ggacttacat ctggggctgg cgccggagca
     5701 ggtgggcgca atgcgcctgg gcgataacaa aagtgcgctg ctgggctgga ccagcttcct
     5761 cggcacaccg gaagaacgac cctcagtcac catcagggta cggtcgtaaa ttatgataat
     5821 cacggagcag aatatgccgg aagagaaact acagacgctt tcattgcagg tcattaacgg
     5881 cagtgagctg gaaagcgggc gggcggcgcg ctgtctgttc acacagcagg gaaatgtcgg
     5941 ccatgccccc gaatgccact ggtcggtaca ggatcgtcag cagagcattc cggcccaggc
     6001 ttttaccgtt atcctgcacg atggcacatt ttgtctacgc ccgcagacgg cacaactgtg
     6061 gctgaatcag gcaaaagtca cagcaacatc agacctgata cagttgcgcc agggcgatga
     6121 gatccagatc ggacggctga tggtgagggt tcatctgaac cggggagata ttccccatta
     6181 cgatgaggaa atggccactc ccgaaaccat cgttaccaat cgcgatatgc tcacggatac
     6241 cctgctatca acggagggtg cgccacacta tccgggaatg actcaccggc accagcttgc
     6301 agacaccgtg gtaaatggtt tttctgccga tccactccag gcacttcagt ccgaaagcct
     6361 gattaccacg ggcgatccgc tttcaggcat tgcggctgtc cggccatcgg caccgctgtc
     6421 cgatccggca agtaatgggg ggatcaatac tccgtttatg gatctgccgc ccatttatgc
     6481 cagccctggc gatcataatg atgacatctc tgcggcagaa atggcgcaac gccaccttgc
     6541 ggtcaccccc ttactgcgcg gtcttggcgg ctcgcttacc gtgagcaatt ccgacgatgc
     6601 ggatgatttt ctggaggagg ccggacgaac gttacaggcc gcaataaaag gtctgctcga
     6661 tttgcagcag cagcgtaaca gcctctcaga caaacatttg cgcccgctgg aagataaccc
     6721 gctgcgcctg aacatggatt acgccaccgc gctcgacgtg atgtttgccg aaggtaaaag
     6781 cccggtacat ctggcggctc ccgccgccgt cagtgaaagc ctgcgcaatg tccgccacca
     6841 cgaagaagct aaccgggcgg cgattgtgga gtcgcttcgt gtcctgctgg atgctttctc
     6901 accacaaaat ctgctgcgcc gctttgtgca gtaccgccgc agccatgaac tgcgccagcc
     6961 gctggatgat gccggagcat ggcaaatgta cagccattat tacgaagaac tggcctccga
     7021 tcgccagcag gggtttgaga tgctgtttaa cgaggtctac gcccaggtct atgaccgggt
     7081 gcttcgtgaa aaacagcggg agccggaagc atgaacagga aggcgtttct ggcctgcgtg
     7141 ctgatgtgcg tcatattaac cggctgcgaa acggcgaaaa aaatcagcga ggtgatcaaa
     7201 aacccggata tccaggtcgg gagcctgaag gagcaacctt cggaaataac cgtcaccctg
     7261 ctgactgaac cggacaccaa taccaatgcg gagggtgaat cagccgccgt ggatgtgcaa
     7321 ctggtgtacc tgactgacga ctcaaaattg caggcagccg actatgacca gatagccagt
     7381 acgccgctac cggatgtgct gggtaaaaac tatatcgacc accaggattt caacctgttg
     7441 ccggacacca tcaaaaccct gccgccgata aagctggatg agaaaacgca gtttattggt
     7501 gtcgtggcct atttctccga tgaccaggcc actgaatgga aacaaatcga gacggtggaa
     7561 ggaaccggcc accattaccg cctgctggtg catgtacgtc aaagcagcat cgaaatgaaa
     7621 aaagaggatg aataattatg gcaaccaccc gcaacaaggt gatgtggcag gaagggatgc
     7681 tgatgcgccc acaccatttc cagcaacagc agcgttacaa cgactacctg gataaccagc
     7741 gtttccgggc catgaatgat ttatcctggg gatttaccga actcaccctc aacaatgaac
     7801 tgctggcgca gggtaagatc atgattgaca gcgcgtcagg cacactgcct gacggcaccg
     7861 tcttttctat ccccgaccag gacgcactgc ccgatccgct gcacccgcaa aattttccgg
     7921 acgaaagaag ccgcaatatt tacctcgctc tgccggtcgc cagtgatgtg agaaatgaaa
     7981 tcagcgacgg gcggcgaatc gggcgttacc ggctgaatta tgccgatgtc cgggatctgc
     8041 attcagaaga aggcgacgcg cgaacactga cgctgggaca actgacgccg cgcattatga
     8101 gcggtgcaga agatatgagt gcctatatta cgctcccgct ttgccgtatc agcgatcgcc
     8161 atgccgacgg ctccctgacg ctggatgacg attttatccc ctcctgccag aatattcagg
     8221 tcagtaagaa actgcgtgtt tatctcaaag aggtacaggg ggccattggc ggacgggcaa
     8281 gcgatctggc aaaccgtatt ggctctccgg cgcagagcgg catcgcggat gtggcggaat
     8341 ttatgatgtt gcagttgctt aaccgtaacc agacccggtt tacccatcgc gcccgccgat
     8401 cccagctcca cccggaagat ttctaccttg atcttgccga gttgctgggt gaactgatga
     8461 cctttacaga gccgtcacgc ctgccctgcc cgcttgatgt gtatgatcat catgacctga
     8521 ccaaaacatt taaaacactg ttaccggaag tcaaacgggc gctgcatacc gtactgtcgc
     8581 caagagcggt caatctgccg ctgcatctgc gtgacggcat ctggcaggcc gatgttcatg
     8641 actcggaact gctacagtct gccacctttg tactggccgt ggcagcaaat atgcctgtcg
     8701 atcagatcca gcgtcagttt atccagcagt cgaaaatttc ctcgccggaa aaaatccgca
     8761 atatggtcag tgtgcagatc ccgggtattc ctctgcgcgc actgatggtg gccccccgcc
     8821 agcttcccta ccattccggg ttcagctatt tcgaactcga caagagcggc caggcctgga
     8881 cagaaatggc tgccgccggg gccgttgcac tgcatgtatc cggcagtttc ccggatctga
     8941 acatgcaact gtgggcgata agagggtaag acgatggatg aggggtcact gtctctgcca
     9001 ccatttaccg gatacgacga aaaaagcctg cgcgactacc atctcgcgct tcatggcaac
     9061 agcctgaatc cgatgatcga cgcggcgacg ccgctactcg gtatggtaat gcgcctttcc
     9121 accatgaaca gccagacgat gccggagcat ctttttgctc aggtggtgac agatgttcag
     9181 gccgtggagc aactgcttca ggaacagggc tacgaaccgg gcgtgattat ctcgtttcgc
     9241 tacatcctct gcacgtttat tgatgaagcg gcgctcggta acggctggtc gaacaagaac
     9301 gagtggatca agcagtcatt gctggtccat ttccacaacg aagcctgggg tggcgagaaa
     9361 gttttcatcc tgctggagcg cctgatccgt gaacccaaac gttaccagga tctgctggaa
     9421 tttctctggc tctgtttttc tctgggattt cgcgggcgct acaaagtggc agtacaggat
     9481 cagggggaat ttgagcagat ctaccgccgt ctgtaccacg ttctgcataa actgcgcggc
     9541 gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc aaggcgggcg ttatcagctc
     9601 atcagtcgcc tgactgttaa acatattttt tgtggtggcg ttgtcgtgct ggcgttgttt
     9661 tacctgttct atctgttgcg gctggacagc cagacgcagg acatcctgca ccagctaaac
     9721 aaattacttc gataaggacg tttatgatcc agattgatct tcccacgctg gtaaaacggc
     9781 tgaacctgtt ctcccgccag gcgctggaga tggccgcctc agaatgtatg agccagcagg
     9841 cagcggaaat taccgtcagc catgtgctta ttcagatgct cgccatgcca cgcagtgacc
     9901 tgcgggttat tacccgccag ggcgatattg gcatggaaga gttgcgccag gcgttgacgg
     9961 tagagaacta cacaaccgcc cgttctgcgg acagctaccc ggcgttttcc ccgatgctgg
    10021 ttgagtggct taaagagggc tggctgctgg cgtcggctga gatgcagcac agcgaactgc
    10081 gcggcggcgt gttgctgctg gccttgctgc attcgccgct gcgttatata ccgcctgctg
    10141 ccgcccggct gttgaccggc attaaccgtg accgtctgca acaggacttt gtgcagtgga
    10201 cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg taaaggcgca ggcacactga
    10261 cggacgcctc tgacaccctg cttgcccgct acgccaaaaa catgacagaa gacgcccgta
    10321 acggcaggct agccccagta ctgtgccgcg accacgaaat cgacctgatg atcgacattc
    10381 tctgccgccg ccgtaaaaac aacccggtgg tggtgggcga agcaggcgtg ggtaaaagcg
    10441 cgctgattga agggctggcg ctgcgcatcg tggcaggcca ggtgccggac aaactgaaaa
    10501 acaccgatat catgaccctt gacctgggcg cattgcaggc cggggcatcg gtgaagggtg
    10561 agtttgaaaa acgcttcaag gggctgatgg cggaggtcat ttcctctccg gtgccggtca
    10621 ttctgtttat cgacgaagcg cataccctga ttggcgcggg caaccagcag ggcgggctgg
    10681 atatctccaa cctgctgaaa ccggcgctgg cgcgtggcga actgaaaacc atcgccgcca
    10741 ccacctggag cgagtacaaa aaatacttcg agaaagacgc cgccctgtcg cgccgcttcc
    10801 agttagtgaa ggtcagcgaa cccaacgctg ccgaagccac cattattctg cgtggtctgt
    10861 cggcggtcta tgaacggtct cacggcgtgc tgattgacga tgatgccttg caggccgcag
    10921 caacgctgag cgaacgttat ctctccgggc gtcagttacc ggacaaagcg attgatgtgc
    10981 tggataccgc ctgcgcccgt gtggccatca acctgtcgtc gccgccgaag caaatctcgg
    11041 cgctgaccac tctgagccac cagcaggagg cggaaattcg ccagcttgag cgcgagcttc
    11101 gcatcggact gcgtaccgac acatcacgga tgaccgaggt gctggtgcag tatgatgaaa
    11161 cgctgacggc gctggatgaa ctggaagcgg cttggcacca gcagcagacg ctggtccggg
    11221 agattatcgc gctgcgccag cagttactgg gcgtggcaga ggacgatgcg gcgccgttgc
    11281 cggacgcaga tactgtggag gatacgcagc cagagtcaga gtcagagtca gaacaggata
    11341 ataccggtgc cgtaccggct gatgagaccg acagagaaca gccggaagag accgctgaaa
    11401 cagtttcccc ggtacagcgg ctggcccagc tcactgccga actggacgcc ctgcataacg
    11461 accggttgct ggtctccccg cacgtcgata aaaaacagat tgcggcggtg attgccgaat
    11521 ggaccggcgt gccgctcaac cgcctgtcgc agaatgaaat gtcggtcatc accgacctgc
    11581 cgaaatggct gggcgacacc atcaaaggcc aggacctggc gattgccagc ctgcataagc
    11641 atctactgac cgcacgcgcc gacctgcgtc gtccgggacg cccgctcggt gcgttcctgc
    11701 tcgctggccc cagcggcgtg ggtaaaaccg aaaccgtcct gcaactggca gagctgctct
    11761 acggcggtcg ccagtacctg accaccatca atatgtccga gttccaggag aaacacaccg
    11821 tttcgcggct gattggctcc cctccgggct atgtcggcta tggcgaaggc ggcgtgctga
    11881 ctgaagccat ccgccagaaa ccctactcgg tggtgctgct cgatgaagtg gaaaaagcgc
    11941 acccggatgt cctcaacctg ttctaccagg cgtttgacaa gggcgagatg gccgacggcg
    12001 aaggccgcct gattgactgt aaaaacatcg tcttcttcct cacctccaac ctcggttacc
    12061 aggtgattgt cgaacacgcg gatgacccgg aaaccatgca ggaagccctc tatccggtgc
    12121 tggcggactt ctttaaacct gccctgctgg cgcgaatgga ggtggtgccg tacctgccgc
    12181 tgtcgaaaga gacgctcgcc accattatcg ccgggaaact ggcccgtctg gataacgtgc
    12241 tgcgcagtcg ctttggtgca gaagtggtca ttgaaccgga agtgacggac gaaatcatga
    12301 gccgcgtcac ccgcgcggaa aacggcgcga ggatgctgga atctgtcatc gatggcgaca
    12361 tgctaccgcc gctctcgctg ctgctgttgc agaaaatggc ggctaacacg gcgattgccc
    12421 ggattcggtt gtcggcagtg gacggcgcat ttacggcaga cgtggaagat gctcagaacg
    12481 acgagtccgt cacaaaggat gaaacggttt tatgagcagg ttgcgcgttt tccggcagac
    12541 aggatggttt atcgcgggcc tgatgacggg cttacccgcc actgccgcgc ccgcagaagc
    12601 atcgtcaatg gccggtgtgg cggtcgccgt cgccaccacg accccgccgg atgcgacagc
    12661 cacgttacag gccatgcagt cctgtcggcg ggagtccgcg gcgctggagc ggcttgactg
    12721 ctatgaccac ctgctggcac cgctgtcccc gtcagggttt gacggggcgc tggtcaaagc
    12781 cggttttgtg ggcgaggcct ggacccgcgc cacagagcag gaaaaacgcc gcgagggcaa
    12841 caccacagaa ctgctggtga cgcaggtgcc gggggagcgc ccgacggtgg tgataaccac
    12901 cccggctatc ggccatgttc cgccgcgccc ggtgctgatg ttcagttgcg tggacaatat
    12961 cacccggatg caggtggcgc tgatgcaccc tctggatgtc catgatatcg ccgtcactct
    13021 gaatgccgac agccgggcat tgcgcagcca ctggtttgtg cgcgagaacg gcaccctgct
    13081 ggagtccagc cgggggctgt ccgggattga tgaaattaag caactgttcg gcgcaaaaac
    13141 gctgacagtg gataccggcg cagataacgc cgcaggaaag ctgaccttta acattgacgg
    13201 gctggcaagg gcgattgccc ccctgcgcga tgcctgccac tgggcgggag aataaaaata
    13261 tggcaatgga cttacgcgat ccgaatgtct ggatatcgca cctgctggaa aacctgccgg
    13321 aagaaaaact ggcatcggcg ctgaaagatg acaacccgga ctgggagtat atcgacggcg
    13381 aaatcgtcaa gctggggtct ctggcccacg ctcagcttga tattcccgaa ctacaacgca
    13441 gggggctaca gcttctggct tctgaaagca aagacttcag gctactggca cacctgctca
    13501 gaaccctgca acatgccggt gatccactgc tggcactgca cctgctaacg ctatacgtgg
    13561 aacattactg gactgtggcc gcgccgcaga atatggcgca taaaaaacgc tttgccagcc
    13621 agatcattaa acgttttgaa acgggtattg aaggcttttc acaaaacgct gccacaacgc
    13681 agcgcgatac tctgctgggt gagctggcga aactggcgca gtgctggcag tcacataacg
    13741 tcccggaact ggcacaggct accgatgatc tttttgccct gtaccagcgt acgtttaatc
    13801 gtgcggctcc tgctccggtc cccactccgg cggcctccgg tagttcacca caaaccaccg
    13861 tcacgtctga aagcggcgtg acgcaaccca gtgctccggc tccccaaatc gccatcgaca
    13921 gtcacgacga caaagcctgg cgcgacacgc tgttaaaagt ggcggctatt ttatgtgaac
    13981 gccagccgga ctcgccgcag ggctatcgcc tgcgccgcca tgccctgtgg caatccatca
    14041 ccagtacacc ccaggcggaa agcgatggac gtaccccact ggctgcggtc tctgccgata
    14101 tggtggcgga ttaccagtcc cggcttgcca gcgcggatat ggcgctgtgg caacaggttg
    14161 agaaaagcgt attgctggct ccttactggc tggacggtca ctgtctttct gcacagacgg
    14221 cactgcgtct gggttacaaa caggtggcag acaccatccg cgatgaggtc atccgcttcc
    14281 ttgagcgtct gccccagctt accgggctgc tgtttaatga ccgcacaccg tttctcagtg
    14341 agcagacgaa acaatggctg gctgcttcgc ccgacggcaa agttgcaccg gttgcgcaaa
    14401 tcggtgagga atcgcaggca gccagagcct gttttgctgg gcagggtctg gaggcggcgc
    14461 tgcgatatct ggacatgcta cccgaaggcg atccccgcga tcagtttcac cgccagtacc
    14521 ttgccgcaca gttgacggag gaggcggggc tgatacagct tgcgcagcaa cagtaccgga
    14581 tgttgttgat gatagggagt cagatgatgg tgtctgactg ggagccatca ttacttacgc
    14641 agcttgaaca aaaattcacg gcagaacaat aaaagagaac aggagttttt gtgttcagat
    14701 taccaacacc ccgactactc agcggactca aatcagccct gcgaccggcg atgccccggt
    14761 ttaaagtctc cgctttctgg ctgctgatac tggcgtggat ttttctgctt gctgatactg
    14821 gcgtggattt ttctgctggt atggatctgg tggaaaggcc cgacgtggac gctgtatgaa
    14881 gaacagtggc tcaaaccact ggcgaaccgc tggctggcaa cggcggcgtg ggggattatt
    14941 gccctggtgt ggcttaccgt ccgggtaatg aagcgcctgc aactgcttga aaaacagcaa
    15001 aaacagcagc gcgaagaggc cattgatccg ctcagtgtgg agctgaacgc ccagcagcgt
    15061 taccttgacc gctggctgct gcgcctgcaa cgccatctcg acaaccgccg tttcctgtgg
    15121 cagttaccgt ggtatatggt catcggcccg gcgggcagtg gcaaaacgac actgctgcgc
    15181 gaggggtttc cgtcagacat tatttatgcc ccggagggcg cgcgcggcac agaacaacgc
    15241 ctgtacctta cgccccatgt cggtaaacag gcggtgatct ttgatatcga cggcacactc
    15301 tgcgctcccg ctgatgcgga tatcctgcat cgccgtctgt gggaacatgc cctcggctgg
    15361 ctgaaagaaa agcgcgcgcg ccagccgctg aacgggatta ttctgacact cgatttaccc
    15421 gatctgctga ccgcagacaa acgccaccgc gagcatctgt tacagacgct gcgcagccgc
    15481 ttacaggata tacgccagca tcttcactgc cagttaccgg tttacgtggt actgacccgg
    15541 cttgatttat tacagggttt tgccgccctg ttccagtccc tgaacagaca ggatcgcgat
    15601 gcgattctgg gcgtcacgtt cacccgccgt gcccatgaaa atgatgactg gcgaacagag
    15661 ttgaatgctt tctggcagac atgggtggat cgaatgaatc tggcgttgcc ggatctgatg
    15721 gtcacccaga ctcacacccg cgcgtcttta ttcagttttt cccgccagat gcagggaagc
    15781 cgtgaaccgc tggtgtcact gcttgagggt ctgcttgacg gtgaaaatat gaacgtgatg
    15841 ctgcgtggtg tctatctcac ctcttcgctt cagcgcgggc agatggatga tatattcacc
    15901 cagtctgccg cccgccagta ccgactgggc aataacccac tggcgtcctg gcctctggtg
    15961 gacaccgcgc cttatttcac ccgcagcttg ttcccgcagg cattactcgc agagcctaat
    16021 ctggcaacgg agagccgcgc ctggctgatg cgttcccgtc gccgcctgac ggttttctct
    16081 gccaccggcg gcgtggcggc actgctgctc atcaccggct ggcatcacta ttacaacggt
    16141 aactatcagt ccggcatcac cgtgcttaag caggccaaag cctttatgga tgtgccgcct
    16201 ccacaggggg aagatgacta cggcaacctg caactgccgc tgctgaaccc ggtacgcgat
    16261 gccacactgg cctatggcga ctggggcgac cgcagccgtc tggccgatat gggactgtac
    16321 cagggacgac gtatcggacc ttatgtggaa cagacctatc tgcaactgct ggagcaacgt
    16381 tacctgccct cgctgtttaa cgggctggtt aaagcgatga atgccgcgcc gccggagagt
    16441 gaagaaaaac tcgcggtgct gcgcgtgatg cgaatgctgg aggacaaaag cggacgtaac
    16501 aatgaggtgg tgaagcagta tatggcaaaa cgctggagcg aaaagttcca cggccagcgc
    16561 gatatccagg cacaactgat gtcccatctt gactacgcgc tggctcatac tgactggcac
    16621 gcagagcgtc aggcgggcga cggtgacgcc atcagccgct ggacgccata tgacaagccc
    16681 gtggtatcag cacagaaaga actgagcaaa ctgcctgtct accagcgggt ttaccagagc
    16741 ctgaaaacgc gggcgctggg cgttcttcct gccgacctca atctgcgtga ccaggtaggg
    16801 ccaacctttg accaggtgtt tacatctgcc gatgacaaca aactggttgt tccacagttt
    16861 cttacccgtt acggcctgca aagctatttt gtaaaacagc gcgatgaact ggttgaactg
    16921 acggcgatgg attcctgggt acttaacctt acccgcagcg tgaaatacag tgacgccgac
    16981 cgcgcggaaa tccagcgcca gctcaccgag cagtatatca gcgactacac cgccacctgg
    17041 cgggccggga tggacaatct gaatatccgc aattttgagt ccatcggaca actgaccggg
    17101 gcgctggagc aggttatcag cggcgaccag cctttgcagc gggcgctgac cgtgctgcgt
    17161 gacaacacac agccaggcgt cttttctgaa aaactctctg ccaaagaacg ggatgaggcc
    17221 ctggcagagc cggactacca gttactcacc cgtctcgggc atgagttcgc cccggaaaac
    17281 attaccctgg cagtacagaa agacaaagaa agcacgatgc aggccgtgta tcagcaactc
    17341 accgagttgc accgctacct gctggcaatc cagaacgcgc ctgtaccagg gaaatcggcg
    17401 ctgaaagccg tgcagttacg gcttgatcag aacagcagcg atccgatatt cgccacccgc
    17461 cagatggcaa aaacgctgcc tgctccgctc aaccgctggg ttggcagact gactgaccag
    17521 gcctggcatg tggtgatggt ggaggctgta cattatatgg aagtggactg gcgcgacagc
    17581 gtggtgaaac cgtttaacga gcaactggca aataactatc cgtttaatcc gcgttctgca
    17641 caggatgcct cactggatgc cttcgaacgc ttctttaaac cggatggcat actggatacc
    17701 ttctaccagc agaacctgaa gctgtttatc gataatgacc tgagtctgga ggatggcgat
    17761 aacaacgtca ttattcgcga agatattatt gcgcaactgg aaactgcgca gaaaatccgt
    17821 gacatcttct tcagcaaaca gaacggtctg ggaacatcct ttgccgtgga aacggtatcg
    17881 ctttcaggca ataaacgccg cagtgtactg aaccttgacg gtcagttagt cgattacagc
    17941 cagggccgta actataccgc ccatctggtc tggcctaaca acatgcgcga aggcaacgaa
    18001 agtaagctga cgctcatcgg caccagcggc aacgcgccgc gcagtatcag cttcagcggg
    18061 ccgtgggcgc agttccgcct gttcggggcc ggacaactga ccggagtaca ggatggcaac
    18121 tttaccgtgc gctttagcgt ggacggtggc gcgatgacct accgtgtgca taccgacacg
    18181 gaagataacc cgttcagcgg tgggttgttc agccagtttg gtctgtcaga cacactgtac
    18241 tgaggaggca gggatgaata gtaacgtact gacacaaact atcgttaccg gcagtgaccc
    18301 gcgcgggctg ccggaattca gcgccatccg cgaggaaata aacaaagcca gccacccgtc
    18361 acagcctgag ctgaactgga aactggtgga gtcgctggcg ctggcgattt ttaaagccaa
    18421 cggtgtggat ttacacaccg ccacctacta tacgcttgcc cggacacgga cacagggact
    18481 ggcgggattc tgcgaaggtg cggaactgct ggcggcaatg gtaagccacg actgggataa
    18541 gttctggccg cagggcggcc cggcgcgtac tgaaatgctg gactggttta actcccgaac
    18601 cggcaatatt ctgcgtcagc aaatctcctt tgcggaatcc gacctaccac tgatctaccg
    18661 cacagagcgg gcattgcagc ttatctgcga taagctccag caggtggaac tgaagcgcgt
    18721 tccgcgcgtg gagaatctgc tctattttat gcagaacacg cgtaaacggc ttgaacccca
    18781 gctgaagagt aacactgaga acgccgcaca gaccacggtc agaacgctga tttatgcccc
    18841 ggaaacacag gcatcttcca caccagaagc ggtagtgcct cccctgcccg gcctgcctga
    18901 gatgaaagtg gaagtgcgca gtctgacaga gaatccccca caggccagtg tgataaagca
    18961 aggcagtacg gtaagagggt ttatcgcagg gatcgcctgt tcagtggctg tcgcctcagc
    19021 attgtggtgg tggcaggtct atccggtgca gcagcaactg ttacaggtta acgacaccgc
    19081 tcagggcgca gcaacggtgt ggatggcctc acctgaactc gaaaactatg agcgcaggct
    19141 gcaacaactt cttgatacct ccccggtaca gccgctggaa accgggatgc agatgatgcg
    19201 tgttgccgac agtcgctggc cggaaagcct gcaacagcaa caggcctcga cacaatggaa
    19261 tgaggcactc aaaacccgcg cacagagtag cccgcagttg cgtggctggt tgcagacccg
    19321 ccaggactta catgcttttg cagatctagt gatgcagcgc gagaaagagg gactaaccct
    19381 ttcctatatc aaaaatgtca tctggcaggc ggagcgggga ctggggcagg aaacacccgt
    19441 tgagtctctg ttgacgcagt accaggatgc ccgtgcgcag aagcagaata cagatgcgct
    19501 ggaaaaacaa attaatgagc gactcgaagg cgtgttaagc cgctggctgc tgctgaagaa
    19561 taatacgata ccgacgataa aaaaagcatt gaatttcaat aacattcatg aatacaaagg
    19621 agttttaaat ggcgaattta atttatttaa cactaaatgg tgataatcaa gggctcattt
    19681 caagtggttg ttcatcacaa ccatccattg gaaataaagc tcaaacagca cacattgatc
    19741 aaattatgat ttatgaattc atgcatggat taaatcgaga tcaaaacgtc aaccatcatc
    19801 atttgacaat aaaaaaacct atagataaag catcaccctt attaggcaaa gcaatttgcg
    19861 acaatgaatt attgacatgt gatttttcct tttatcgtac aaataaattt ggaattaatg
    19921 aactgttcta taaaatcaaa ttaactggag caaagataag cgacattcat gtctctatat
    19981 cacatattgt tgttgataac agcgttcaac ccgaagagtc agtttctttc agttatgagt
    20041 caattatatg ggagcattgt tccgctggca ctagtgcata cagtttatgg gaagatcgtc
    20101 ttttttaaaa acatatggga ttattataat ggctaacaat attttacact cttcaacatc
    20161 agacagtgaa agcaaaaata caagcactga cagtacgtta tttaatgaaa aattcccatt
    20221 tcaactaaga caagatttcg catcaagaat aacagaagat gaaagtgatt tttttaaatg
    20281 gaaaaaaaat aagagagttg gcatttgtga gcaatcataa agaatggaat aaatatgact
    20341 tattaattta aaaatctgtt aatgaaatca acattcattt gtccagcact ccatactttc
    20401 agccgttgga ttggtatata ataaaagcca tgctctggac tgaaaacgat gcggaaaata
    20461 cttctcagtg gaatggttat ccattacaaa ttggtcgttt tcggaaagat aaagcgatgc
    20521 cagctttaat ctctggagaa aaatctacag cccttgttac cccaccacaa tggagaaaca
    20581 aagcattcaa tgggttaaaa gatccagaaa gaaattactg ggcaaaagaa caaataactg
    20641 gctctccaga agagaatatc aaagcagcaa ttacttactt aatgatgaaa ctttctaata
    20701 caaaggaaga atcaactatc gatcaatatg actcaactct atatagcgcc attgtccaaa
    20761 aaggcgattt ggctgacaac atcagaaaag agagaaaaac gaccatacct aacttaacga
    20821 aaaacaatcc tggaaaaaat ctcgataaaa tacacccggg agatatttta tattatcaaa
    20881 aagcatccat gaaagtaata attactggat ggaaacctat cacaataaaa aatgtagcga
    20941 tgaattataa tggtggtggc gatcccaagt atgcaataaa acttcagttc gtttatacat
    21001 tactcacaaa aaacagagta ttatgagaat gcgaaaaata acaattattc tactgctgac
    21061 attctcatgt acaaaaggtc tggcatttga catttccatg tctaaagaat gtagtggtgt
    21121 tgatgaactt gaatttattg attgtgttaa aaactcattt attatgagca ctattacact
    21181 taaaaaagca gaagcaaata taaaagagaa aattcaaaaa tggaatagtg atgaggacga
    21241 agcaataaga aaagaagcag ttatttcttt aaaaagacta gagtcttcta atagggaatt
    21301 tatacgttac agatttgctc aatgctcttt tactctaacc tggggggcca gagtagggcg
    21361 actggca
//
LOCUS       DABSYT020000078        20200 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.078, whole genome shotgun sequence.
ACCESSION   DABSYT020000078 DABSYT020000000
VERSION     DABSYT020000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.078"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(91..366)
                     /locus_tag="ID725_RS17135"
                     /old_locus_tag="pgaptmp_003427"
     CDS             complement(91..366)
                     /locus_tag="ID725_RS17135"
                     /old_locus_tag="pgaptmp_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HGV7851945.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(427..1788)
                     /gene="espK"
                     /locus_tag="ID725_RS17140"
                     /old_locus_tag="pgaptmp_003428"
     CDS             complement(427..1788)
                     /gene="espK"
                     /locus_tag="ID725_RS17140"
                     /old_locus_tag="pgaptmp_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HGV7851946.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            complement(2152..3015)
                     /gene="potB"
                     /locus_tag="ID725_RS17145"
                     /old_locus_tag="pgaptmp_003429"
     CDS             complement(2152..3015)
                     /gene="potB"
                     /locus_tag="ID725_RS17145"
                     /old_locus_tag="pgaptmp_003429"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGV7851947.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(2999..4135)
                     /gene="potA"
                     /locus_tag="ID725_RS17150"
                     /old_locus_tag="pgaptmp_003430"
     CDS             complement(2999..4135)
                     /gene="potA"
                     /locus_tag="ID725_RS17150"
                     /old_locus_tag="pgaptmp_003430"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGV7851948.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            4385..5611
                     /gene="pepT"
                     /locus_tag="ID725_RS17155"
                     /old_locus_tag="pgaptmp_003431"
     CDS             4385..5611
                     /gene="pepT"
                     /locus_tag="ID725_RS17155"
                     /old_locus_tag="pgaptmp_003431"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGV7851949.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(5660..6781)
                     /gene="roxA"
                     /locus_tag="ID725_RS17160"
                     /old_locus_tag="pgaptmp_003432"
                     /gene_synonym="ycfD"
     CDS             complement(5660..6781)
                     /gene="roxA"
                     /locus_tag="ID725_RS17160"
                     /old_locus_tag="pgaptmp_003432"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGV7851950.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            7030..8259
                     /locus_tag="ID725_RS17165"
                     /old_locus_tag="pgaptmp_003433"
     CDS             7030..8259
                     /locus_tag="ID725_RS17165"
                     /old_locus_tag="pgaptmp_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7851951.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            8624..8812
                     /locus_tag="ID725_RS17170"
                     /old_locus_tag="pgaptmp_003434"
     CDS             8624..8812
                     /locus_tag="ID725_RS17170"
                     /old_locus_tag="pgaptmp_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7851952.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            8870..9424
                     /locus_tag="ID725_RS17175"
                     /old_locus_tag="pgaptmp_003435"
                     /pseudo
     CDS             8870..9424
                     /locus_tag="ID725_RS17175"
                     /old_locus_tag="pgaptmp_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            9617..9814
                     /locus_tag="ID725_RS17180"
                     /old_locus_tag="pgaptmp_003436"
     CDS             9617..9814
                     /locus_tag="ID725_RS17180"
                     /old_locus_tag="pgaptmp_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGV7851953.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            9807..10019
                     /locus_tag="ID725_RS17185"
                     /old_locus_tag="pgaptmp_003437"
     CDS             9807..10019
                     /locus_tag="ID725_RS17185"
                     /old_locus_tag="pgaptmp_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851954.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            10009..10473
                     /locus_tag="ID725_RS17190"
                     /old_locus_tag="pgaptmp_003438"
     CDS             10009..10473
                     /locus_tag="ID725_RS17190"
                     /old_locus_tag="pgaptmp_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851955.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            10466..10699
                     /locus_tag="ID725_RS17195"
                     /old_locus_tag="pgaptmp_003439"
     CDS             10466..10699
                     /locus_tag="ID725_RS17195"
                     /old_locus_tag="pgaptmp_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851956.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            10705..11004
                     /locus_tag="ID725_RS17200"
                     /old_locus_tag="pgaptmp_003440"
     CDS             10705..11004
                     /locus_tag="ID725_RS17200"
                     /old_locus_tag="pgaptmp_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGV7851957.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            11001..12401
                     /locus_tag="ID725_RS17205"
                     /old_locus_tag="pgaptmp_003441"
     CDS             11001..12401
                     /locus_tag="ID725_RS17205"
                     /old_locus_tag="pgaptmp_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HGV7851958.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            12602..12853
                     /locus_tag="ID725_RS17210"
                     /old_locus_tag="pgaptmp_003442"
     CDS             12602..12853
                     /locus_tag="ID725_RS17210"
                     /old_locus_tag="pgaptmp_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851959.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            12850..13260
                     /locus_tag="ID725_RS17215"
                     /old_locus_tag="pgaptmp_003443"
     CDS             12850..13260
                     /locus_tag="ID725_RS17215"
                     /old_locus_tag="pgaptmp_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGV7851960.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            13271..13543
                     /locus_tag="ID725_RS17220"
                     /old_locus_tag="pgaptmp_003444"
     CDS             13271..13543
                     /locus_tag="ID725_RS17220"
                     /old_locus_tag="pgaptmp_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGV7851961.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            13500..13628
                     /locus_tag="ID725_RS17225"
                     /old_locus_tag="pgaptmp_003445"
     CDS             13500..13628
                     /locus_tag="ID725_RS17225"
                     /old_locus_tag="pgaptmp_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGV7851962.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            13670..13894
                     /locus_tag="ID725_RS17230"
                     /old_locus_tag="pgaptmp_003446"
     CDS             13670..13894
                     /locus_tag="ID725_RS17230"
                     /old_locus_tag="pgaptmp_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851963.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            14146..14352
                     /locus_tag="ID725_RS17235"
                     /old_locus_tag="pgaptmp_003447"
     CDS             14146..14352
                     /locus_tag="ID725_RS17235"
                     /old_locus_tag="pgaptmp_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851964.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            14352..15407
                     /locus_tag="ID725_RS17240"
                     /old_locus_tag="pgaptmp_003448"
     CDS             14352..15407
                     /locus_tag="ID725_RS17240"
                     /old_locus_tag="pgaptmp_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGV7851965.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            15420..15755
                     /locus_tag="ID725_RS17245"
                     /old_locus_tag="pgaptmp_003449"
     CDS             15420..15755
                     /locus_tag="ID725_RS17245"
                     /old_locus_tag="pgaptmp_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGV7851966.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            15768..16181
                     /locus_tag="ID725_RS17250"
                     /old_locus_tag="pgaptmp_003450"
     CDS             15768..16181
                     /locus_tag="ID725_RS17250"
                     /old_locus_tag="pgaptmp_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HGV7851967.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            16387..16929
                     /locus_tag="ID725_RS17255"
                     /old_locus_tag="pgaptmp_003451"
     CDS             16387..16929
                     /locus_tag="ID725_RS17255"
                     /old_locus_tag="pgaptmp_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGV7851968.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            17185..17466
                     /locus_tag="ID725_RS17260"
                     /old_locus_tag="pgaptmp_003452"
     CDS             17185..17466
                     /locus_tag="ID725_RS17260"
                     /old_locus_tag="pgaptmp_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851969.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(18067..19527)
                     /gene="phoQ"
                     /locus_tag="ID725_RS17265"
                     /old_locus_tag="pgaptmp_003453"
     CDS             complement(18067..19527)
                     /gene="phoQ"
                     /locus_tag="ID725_RS17265"
                     /old_locus_tag="pgaptmp_003453"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGV7851970.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(19527..20198)
                     /gene="phoP"
                     /locus_tag="ID725_RS17270"
                     /old_locus_tag="pgaptmp_003454"
     CDS             complement(19527..20198)
                     /gene="phoP"
                     /locus_tag="ID725_RS17270"
                     /old_locus_tag="pgaptmp_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGV7851971.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
BASE COUNT         5417 a         4635 c         5130 g         5018 t
ORIGIN      
        1 gttcatggta aaaccacctc ctgtggaggt ggttgcttgc agtcaggtgt ttcgggtctg
       61 tggctttttg tgttctctgg taagtggttg ctattctttt gtgttgtgta tctctgtttc
      121 tatagtttta gatagccatg tcatctcagt aactgagagt tttagttttg gggttctttc
      181 tcctgcgccg ggtgggcgca tgtcaaaact acaggctttt ttttggtaca ctgtatagct
      241 atctacatta catgttgttg atgtaagtat gcagttcttt gacgctggct tgtcttttat
      301 gggaccggat atttccgggt aatgtacttg tgaacttgaa aatctgtttt tgattgaaaa
      361 tggcattgtt ttctcctttg ctccatgaaa agtgttttta tgctattccg ttataagttg
      421 gtatcattaa gaatatttat atgtggaacc agagtatttt ggacgatagt ctttccattc
      481 tggtgtatct aatattcttt ctgctggtat ctctttgcca tgtagataaa attttatgtt
      541 tttcattatt tggttgttgt ctttattacg gtaaatccat cgtaattcgc tggcggtaat
      601 actctggcca tagccctctg acttgttagc tatgagtttg agtgagttga caagctcatc
      661 cgcacaaaaa ataaccgttt tattgcgtgt taatgtctga aactctaggc cggccttgct
      721 tgtttttgcc cataatttgt cgcgattgta tacggaaagg tcggccatgg taacattata
      781 attattgtga ttctttaagt gttgtttaaa ttttattcct ctttcgtgtt catttaatgc
      841 tcttttgata agtggatctt gcttatattt gcgaggtgtt ctggggtccc atactgcgtt
      901 tgttaacgta ttctgagtta taatatcttt tttggaaaag tcgttaacta gtttaatgct
      961 gttgacaagt ttttctctgg gtgaagatag tccatagaca atgtctccct cgcgaaagtg
     1021 taaggccaca tccagagcgt tgccctgaaa ggtgatgttt tttgctttaa ctacatctgg
     1081 agagagtagt acattttgta aatcaagatc cagcccatca ggcatccctt caacaccgat
     1141 gttccacgtg gttttctgct ccgcatgaat ggttaccgat gtcacactct ccggaagttt
     1201 ctccggcagt ataatattgc tacatccagt cagcgatagc gtcttcagtg acggtgatat
     1261 caggttgtca attctggcct gattctccgg gttatagcta ttgatgctga gagatgataa
     1321 cccgtttgga acatttttta tgctgtctgt ggcgctacct tttatttcaa ggtagcggac
     1381 actctctggc actccagata tatagcagtg gcaaaccgtc aggcactcga tgccttcaga
     1441 tatgaaatgt ataaatggtg aatcagttaa ttcaatatgt agatttttta cggttgatgg
     1501 catgtctgtg atttgtaatt tttcacagtt aatgacggag aggttttgtg tattaaccgg
     1561 caatcctgta agggttgtca ggttgttgca gttttcgatt gtaactgatg ttatttcgtt
     1621 tggtaactgt ggcagatact caatatcaca atctttgata tataaacgac cactggcccg
     1681 cgcttcttct atctgaattg gtgtgatttc gcttttgatg tctgctgatg tttcagcgga
     1741 ataggagcag aatgtcccgg tcggtcgtaa ttgcgatgta ggaagcattg ataaattcca
     1801 tttttagaaa taaataacag agtaagttct atccatacta ctaatcaaca agctaatttt
     1861 aacaaccagc aaagccaagg tgtcaatatt agaaaagaga gttatatctc aaaaccaaca
     1921 gactgttgat tttaactttt gcgaatactt tctccagtcc gtcatgcatg gattaggatt
     1981 gctcattttt ataccatttg ttgttttttt atagcgattt gctaaaaggg agaagaaaaa
     2041 acgttctcca aaattagatt gcaactgttt gattttatgg atagaggcat gctgtttact
     2101 agacagtaaa aagcatgcca aatttcctat taaccttttg aatataaagc actaattatc
     2161 tatttctgtt gccttcttat tcagcagacg agaagcgcgc cagtaaacca gcaacatcag
     2221 gcccattacg atagtcagcg taatgctggt ggctgcacca aacggccagt cacgaatatt
     2281 gaggaactgt accttgatga cgttaccgat cagcaggttt ttcgcaccgc ccatcaggtc
     2341 ggacacatag aacaggccca tcgctggcag catcaccagc agacatccgg caataatccc
     2401 cggcatcgtc agtggaataa tgatgcggat aaaagtctgt aacttgctgg caccgagatc
     2461 gcgcgccgcc tcaagcaacg gcttatccag tttttcgata ctggagtaca gcggcatcac
     2521 cataaacggt agcagaatgt aaaccagacc gataatcacc gcactgggcg tgaacatgat
     2581 acgaattggt gtgtcgataa cgcccagcca gagcaaaaac tcgttgagat agcctttggt
     2641 gctgaggaaa attttcagcc cgtagatgcg aatcagtgag ttggtccaga agggaacaat
     2701 cagcagaaac agcagcagcg gacgcacctt gtgtggcagc ttcgccagaa accaggcaaa
     2761 cggatagccc agcaccaggc aggcgagggt ggcgatcagc gccatattca gcgagtgcaa
     2821 tagcacttca aaatagagcg gatcgagcag acgtgtgtag ttatccagcg taaagaccat
     2881 tttgacaaaa ctggcgtcgt cgcgggccaa aaagctggtg ccaatgatca tcaggttggg
     2941 cagaaagaca aacaacacaa gccaaccgac aatagtgaca atcactacat tctggaactt
     3001 acttgtgttc ttcatcagcc agtacgacct cccagctttc tacccaatta atggccattt
     3061 tttggtcgag agagtggtca aagtcaggat cgtcttcatt gaagaattcg ctgaccatca
     3121 ccatcttgcc attttccagt tcaacaaccg actccagcgt catgcctttg tagttacgct
     3181 cgcgaacgta accaatcagc ccttcagcgt ggttgtcgtc gttaatctct tcaacacgta
     3241 agtcttccgg gcgcagcaga acatgcagtt tttgccccgg ttcaacggcg aagttaacgt
     3301 agatattaca ttcgcggcct tcaacgttgg cgcgtacgcg ctgctcgtcg agacgttcga
     3361 tgacagtggc gttaaacata ttgatttcgc caatgaaccc ggcaacaaac aggtttttcg
     3421 gctcttcgta gatttcacgc ggcgtgccgt cttgctcaat gcgaccatcg cgcatcacca
     3481 caatcctgtc tgacatggtg agtgcttctt cctgatcgtg agtcacgaag acgaatgtaa
     3541 tgccaagctt acgctgtaac gctttcagct cgttctgcat ttgcttacgc agtttgtaat
     3601 ccagcgctga gagcgactca tccagcaaca acagacgagg cttgttaacc accgcgcgag
     3661 caatggcgac gcgttgctgt tgaccaccag agagctgatg cggtttacgt tgagcgaagg
     3721 tttccagctg caccatccgc agggcttcca tcacgcgggg cgtaatttca gcagcggggg
     3781 ttttttgcat gcgcaaccca aaggccacat tttcgaacac ggtcatgtgg gggaaaagtg
     3841 cgtagctttg gaaaacagtg ttcacatagc ggttttccgc cggaacgtgg gtgatgtcct
     3901 cgttatccaa catgatgcgt ccggaatcaa cagtttccag acctgcaatc aggcgaagaa
     3961 cggttgtttt accgcagcca gaagggccaa gcagcgtgag gaactcgcca ttgttgatag
     4021 tcagatccag ctggggaatg acctctttac catcaaagca tttgcgaatt cccgccaatt
     4081 gcaccagcgg tgaaagcgaa ctcggttgtt tattcaattt tttactctgt cccatgtaaa
     4141 cgcaacggat ggcttaccga tgcggggttt gtggttaacc accttggtga ctcttaatga
     4201 gggcggtaat tctacggcaa accgcttgaa tcgccaatct ttgttgtgaa ttactggctt
     4261 agctttatat tcattaaggt aatgctgata aatattcccg cttgcagggg taaaagtgac
     4321 ctgacgcaat atttgtcttt tcttgcttct taataatgtt gtcacaaaaa gtgagggtga
     4381 ctacatggat aaactacttg agcgattttt gaactacgtg tctctggata cccaatcaaa
     4441 agcaggggtg agacaggttc ccagcacgga aggccaatgg aagttattgc atctgctgaa
     4501 agagcagctc gaagagatgg ggcttatcaa tgtgacctta agtgagaagg gcactttgat
     4561 ggcgacgtta ccggctaacg tccctggcga tatcccggcg attggcttta tttctcatgt
     4621 ggatacctca ccggattgca gcggcaaaaa tgtgaatccg caaattgttg aaaactatcg
     4681 cggtggcgat attgcgctgg gtatcggcga tgaagtttta tcaccggtta tgttcccggt
     4741 gctgcatcag ctactgggtc agacgctgat taccaccgat ggtaaaacct tgttaggtgc
     4801 cgatgacaaa gcaggtattg cagaaatcat gaccgcgctg gcggtattgc aacagaaaaa
     4861 cattccgcat ggtgatattc gcgtcgcctt taccccggat gaagaagtgg gcaaaggggc
     4921 gaaacatttt gatgttgatg ccttcgatgc ccgctgggct tacactgttg atggtggtgg
     4981 cgtaggcgaa ctggagtttg aaaacttcaa cgccgcatcg gtcaatatca aaattgtcgg
     5041 taacaatgtt catccgggca cggcgaaagg agtgatggta aatgcgctgt cgctggcggc
     5101 acgtattcat gcggaagttc cggcggatga aagcccggaa atgacagaag gctatgaagg
     5161 tttctatcac ctggcgagca tgaaaggcac cgttgaacgg gccgatatgc actacatcat
     5221 ccgtgatttc gaccgtaaac agtttgaagc gcgtaaacgt aaaatgatgg agatcgccaa
     5281 aaaagtgggc aaagggttac atcctgattg ctacattgaa ttggtgattg aagacagtta
     5341 ctacaatatg cgcgagaaag tggttgagca tccgcatatt ctcgatatcg cccagcaggc
     5401 gatgcgtgac tgcgatattg aaccggaact gaaaccgatc cgcggcggta ccgacggcgc
     5461 gcagttgtcg tttatgggat taccgtgccc gaacctgttc actggcggtt acaactatca
     5521 tggtaagcat gagtttgtga ctctggaagg tatggaaaaa gcggtgcagg tgatcgtccg
     5581 tattgccgag ttaacggcgc aacggaagta agcgaaaagg gatgcggcat gtgatgccgc
     5641 atccggctta aatccaaact tacccttcga agaaccaata cccgctattg accagcgccg
     5701 cgagcatcgc gaggaatgac ggatcttcca gcgcatcgcc aaaattctcc gcagtcagcg
     5761 caatgttgct ggcgagtgca tccagtgccg gacggtgcgg ggaatcgatc ttctcaccat
     5821 tggtatacac gtcgtcgcca atgcgcaata cgcgcagacc acccaggcgc accagcacat
     5881 caccttgttt cagcgcatcg tagatttcat ctggctgata aggcggctcc ggcggcgcga
     5941 tatccagttc atgacgtgac tgggatataa actcgccaaa ccattgctta aagtgttccg
     6001 gctggttgat caattcgagc atcatctcac gcagtttatc catctcttgc ggcagaacgt
     6061 ccgcaggatg agcgcgaggt ggaacatcag gatcgctgta gtagttgccg cccagttcgc
     6121 gttgcagcac ataatcggca aatccgctaa ttaattcccg cgtatttggc gcgcgaaaac
     6181 ccacggaata gttcatcgca ttttccagcg cgtagccttc atgcgggaat cctggcggaa
     6241 tataaagaat atcgccaggc tccagctctt catcgatgat ggcttcgaac ggatcgacct
     6301 gtaacagatc cggatgtggg cagtgctgtt tcatttgcag cttctcgccc actcgccagc
     6361 gacgacgtcc ggtaccctga atgataaaca cgtcgtactg atcgagatgc gggccgacgc
     6421 cgccgccagg tacagaaaaa gagatcatca gatcatcaat acgccagtcc ggtagttcac
     6481 ggaacggtcg catcagcgcg gcggtcggct catgccagtg gttcactgct tgcactaaca
     6541 atgaccagtt agtttcaccg agatgatcgt agctttcgaa cggaccgtgg ctgacctgcc
     6601 attttccatc ctggtgactg accagtcgac tgtcgacttc gctttccatc gccagacccg
     6661 ccaactcgtc tggagagatc gggtcaataa aattattaaa gccgcgtttt aacaccaccg
     6721 ggcgtttctg ccagtgacgt tcaagaaaat cgggccagtt aagagtgagt tggtattcca
     6781 tgttaagcat ccacaggctg gtatctgcaa ccgattataa cggatgctta acgtaatgcg
     6841 tgaagtatgg acatatttta tcaattagtg ttttttgccg tttcataatg tttcagattg
     6901 ttcattatat ttttgattat aaacagattt gtgattttcc ttgttccagc ttgtttcacg
     6961 ttgtagccct ttgtgcaacg atcggtgtag ttaatggtgt agttaattgt gatgaccgga
     7021 atcaaaatta tgagcagagc acttaacaaa ctgagcgata cacagctgag gaaaatcaac
     7081 ggcacacccg cccaaaaaac agcctttctt aatgacggtg gaaacctgag cgtcaggcat
     7141 tcaaccagtg gccttttaac ctggtatttc acttacaggg ccggaacggg aaggggggca
     7201 ccaccggaac gcattaagct gggaaattat cctgatctga gcctgaaatc agccagggaa
     7261 aaagccgccc agtgtcgcgc atggctggca gaggggaaaa atccacgtca tgagcttaat
     7321 tacaccgtac aggaagcgtt aaagccggta acggttggcg atgcgctcac ctactggctt
     7381 gagtcgtacg caaaggaaaa ccgcgtggat tatgccgccc tgaaaaagcg ccttaataat
     7441 cacgtaatac agcacattgg tgctatgccg ctggataaat gcgagctacg gcactggctg
     7501 gcctgttttg accaggtggc aaagcgaacg cctgttactg ccggattctt gctacagacg
     7561 tgcaaacagg cgcttaagtt ctgccggagg cggcgctatg caatcagcaa cgttcttgat
     7621 gatatgagtg tggcggacgt tgggaaaaaa ccggatataa gcgagcgtgt cttaagcacc
     7681 aaagaactgg gcgaattatt gcaggcactg gacaaaaaaa tattctcccc ctactacatc
     7741 gcgttaatcc gcctcctgat tgtgttcgga tgccggacgg tagaactgag gttatcggag
     7801 atcagcgagt gggattttac cgaaatgctc tggaccgttc cgaaggagca cagcaaaacg
     7861 aaggtagcca tattccggcc tataccggaa gcaatactgc cgttcatcac gcagctggtg
     7921 gagcagaaca ggcacacggg cttattgctg ggggaagtga aacaggaggc cagcgtatcg
     7981 cagtatggaa gattagcgca caggaggctt aatcaccctc actggtcact gcatgacatc
     8041 cggcgcacct ttacaaccat gctgaacgat ttaggcgttg atccgcatgt cgtggagcag
     8101 cttacaggcc accagatgcc aggaatgcag cgagtttata atcattcccg ttatctggat
     8161 gcgaaacgca atgcgctgga tatgtggacg gagcggttag ggatactggc gggaacacat
     8221 gaaaacgtaa ccacgctacc agtagccaga agaaaataat ttttttcgtg ttttttcagt
     8281 atgcgcatac tggatatgcg aacagataca acgtgcaaca atgagcaaca atgtggaaca
     8341 actacgaaca agaggcgaaa aagcgtacgg atttataagt agctgatttt taatgtgtta
     8401 ctggtttttt atacactagc gaatcactct ttaaagagcg agaaaaaagg catggaacaa
     8461 tattttatgc ctttgtatta acatgaattt aaaagatttt attcttactt tttcattttc
     8521 tgtaaatcat acatcctcag tatttgccat tcatcactct agcgggaaca atacgacaca
     8581 ataagacacc tgatgactct ttaagaaacg aaagggggca atagtgttaa gcactgatcg
     8641 gtttatacgt gaaaaagaat gcgaaaaact aaccggcctt agccgtacgt gtcgctaccg
     8701 cctggaaaag gccggacaat tcccatcacg tcgtaaactt ggcggtcgtt ccgttggctg
     8761 gtctttatcc gaggttctgg cctggaagga tagctgcaag gcagttcatt aatcacgctg
     8821 gcggcacaca gccgccacac atcaatcatc tgaacacaga gctataacca tgaagattga
     8881 atatacgcca gaacgtgggc ggggattcgt tcgccctggt gagactggaa aaccacaaaa
     8941 ttggggtttt tcaggcataa aaaaagcggc cccgaaatgg agccgccttt ctgaacagat
     9001 aacccgctgc gccttatgtg tatatgatcc caaacataag cacggggatg atagccgcta
     9061 tcaggctggt gggcaatgct atcagtctgg ttcagttcgt tgccatacct gcaatgggcg
     9121 cgtttccctg tactctttaa ggaattgctc aagggcaaaa gcacatggcg cgaatctttc
     9181 tgattcatgc tctatctttc tgcgccgtct tttccgtgcc ggtgataatg ttttggtcaa
     9241 ttctttatcg gtcattgtgt tgtcctgcat agcaatgcgc cgtaatacct cacaccacgg
     9301 cgctgatagt ttttatcctt tgggttctat gccgcgttgc tgtaattctt tgcggagaac
     9361 gcgtttaatc caagtggcta aagatgtgtc gccatcggcc ttagcagctt cttctagctg
     9421 cgctctgaag tcttccggta atcgcatttg gtatggaggc gaacgttttt ctaatggtgt
     9481 tgacatggta attacccttt attgctatac ctacatggta attaccattg tacttgccat
     9541 tacacaaaaa tcaacgcccc gcagtgctgg gaacacatac ggggcgtcta accaacaacg
     9601 taaactagga gccgttatgg ttgccgtaaa tcatatacca caccttgtac acacacaaac
     9661 ggcctttgtg tggcgttttc tggcactgag tgccggagaa tctcaaatca tccacgtaac
     9721 cgcctggacg gaacgcgaag cgcgtaaccg ttgcccgtcc ggttgtgttg ctgtatttgc
     9781 cgcccgtatt cgtcaggagg tgcgccatga ttaatctgtc acttactgac cttaaccgca
     9841 ttcagtttcg tgagaaattc acagggcagc tactggtcaa tgtggataaa ggtcgcgtgg
     9901 tgtgtaatta ccacttacca gatgaagcaa ttgtcgccac aagggaatca ttacaggaaa
     9961 tcacaaaacg tgccggaatg attaacacga atattcaggg gatgctttat gcacaataaa
    10021 accacaccgg acgcagccgc cgaagccatc aaaacgctga tgcacgcgct gattgatatt
    10081 tctgttatcg cggacagggc acataagcac gccaccagcg aaacagaata tgccggtgct
    10141 ttcgttcctc actcactggc tgtcatgcaa tttagtgctg atacggcgct gaatgaggcc
    10201 aaagctatcc tgattgctga ttgtgaaaat gggggggtta tgcgtgatga tcgttttaat
    10261 tccctgaaac aggaattttc cggcgttcct gatgatgcgg ctgatgcgct ttcgtcaatg
    10321 ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa atcaacgggg
    10381 cttgatgtac tgaatatcgc cgccgattat gcggaatatg tggcagaggc gcgttataga
    10441 agaaaatttc ctgaggatgt aagccatgcg tgatatttac caccaaacaa tagaccgcgc
    10501 atttcttgca ctttctcaca gtgaaaacat gatggaaata ttgcgcatat ggcttgaaac
    10561 acttggcgac aatgaacgcg acaaacaaaa atcaagaatt gccacggcat taataacgct
    10621 tcttgagcct gtaataatgg aactgcaaga aatagatcta ttgcacgaca gatataaaga
    10681 acagcacacc ggagaataaa aataatgaaa cttaaatatt ctggcttaac tgccagtggc
    10741 aacactcacc ctaaatttac gcgcggtgat atttaccgcg accagtacgg cggcacggta
    10801 atgattaagg gcgtggaaga acggcgcgta acctaccgcc gtgaaggtta cgaatatgat
    10861 tgcgtgatgc ctgtttatca gttccggcgt gatttttctc tggtacagac cgcgccgcat
    10921 aacgtgccca ccagcaacgc cagggcacgg gcaaacatcc agaagctgaa aaccatggtt
    10981 aacggattca ggggcaagaa atgaaaagcg caccgaactt aaaaaaacag ccttacgaca
    11041 agatgaccga agtcattatt tttgcgggta gtgatgcctg ggcacatgcg aaacagtggc
    11101 aggaacagga cgggcgactg gctggcgata atgtgcctcc cgttgtgctg gctgatgatc
    11161 aactggatga actggcagaa ctgagaatca tcgacgaggg gcgctattgt gtccggctgt
    11221 acaaggcagg ccacatcagg ccatcaaata ttaatgccat tgcgcacaag ctggcggcgg
    11281 cgggtgtaac tgatgcgaat tattaccccg aagggatgca cagccatatg cgggagaact
    11341 ggcgcgaata cctggaacgg gtgcgcggga aagagccggc ggaagaaaaa aaccaccagc
    11401 gaaaaaccac gctaccgatg agcgttggat ctaccggata cgacacgcaa ctggattacg
    11461 tggttaaggg gattattccg gcggtatcgc tatgcagcat atacggggct agcgggtcct
    11521 ataaatcatt ccttgccgga tcgtgggcgt gccatgttgc cactggtcgc cagtggggag
    11581 gccgcagggt tgcacatggt gcggttctct atgtggttgg tgaaggcggt ataggtgttc
    11641 cgcgtcgtgt aaaagcctgg gaggttgtgc acgatgagca ggtgaaaaat ctgtatctgg
    11701 taaaccgccc catctttccg gctgccccgc ttgatgttga tgaaatggtt atcgctgccc
    11761 gtcaggtgga gcgggaaacg ggtaaacctg tacgcatgat tattctggat acgctggcgc
    11821 gttgctttgg tgggaatgat gaaaatgatt cccgtgatat gggggcgttt atccgtggtt
    11881 gtgacgaact gaaacgacgc acaggggcca cggtgctggt ggttcaccat tccggcaagg
    11941 atgagacgaa aggcgcgcgc ggttccagtg catttcgtgc ttcgctggat gctgaatacc
    12001 ggatacgcag ggaggacgca ggaagcgaag cgctggttat ctcatgcacc aaaatgaagg
    12061 acgcggagga actcaaagaa gccgcatatg acttacgcgt ggtggagctt tttaccgacg
    12121 ctgacggtga attaatcacg tcgctggtgg tggtggatga tccgcgccct cctgttgaac
    12181 tggagcgcat cgaggaggca gggaacaaga cggaaaacca taccgcgcta tgggggtgca
    12241 tccgttcacg cacacagaac ggcgacaagt gcacgatccc gctgttacgt gatgacatga
    12301 aaaagctggg gtatgaaatg aaaaacttcc ggcgctggct gtacaagctg gaaaaagatg
    12361 gggttattcg tatcgatggg gatgatgtag cgccgctata aaagtgagga gcaaaagcga
    12421 gggggataga aagagggcca aaattagccc gctctccctc acttttcgac ctgtatacat
    12481 cctcaaaagt gaggggtaaa aaaatactta tgaaacacac acatagaaaa accgaaaatc
    12541 ccaactgcga cgaagtgaga cgcttgaaaa agtgaggcga aaaagtgaga ggttgcgaga
    12601 aatgacccaa aaacgcagag acagaacaga gccaaaatat aaagcgttag acatgactga
    12661 gcacacctta aaggtggcaa tcagaacgat agaccgccac acgcgggaag gatacgcgaa
    12721 ggaacatccc gacctgataa gcgcattcat gaccacggcg gcggcaaact ttgccacgct
    12781 gacagaacgg gagattgccg aagcggaaca ggtaacaacc atcaacgtta aaaccggaga
    12841 ggtggaatta tgacggcaca gatagccgct tacgggcggc tggtggacga cccgcaggta
    12901 aaacagacca gcaagggcac accgatgacg ctggcgcgta tggcggtatc tttgccatgc
    12961 agccaggcac aggacggaca ggcgacgtta tggttatcgg tcatcgcatt tggcaaacag
    13021 gccggcttcc tggctaaaca tcaaaaaggc gatgttgcca gcgtatccgg cacgatgcag
    13081 gtaagccagt ggaccggaca gaacggagaa acgcggcagg gctggcaggt tatcgcagac
    13141 agcgtaatca gtgcccgcgc ggcacgtcca ggcgggaaca gacgcaaaac cacaggcaca
    13201 cagggtaatc agccaccagc gggaggcgat gacccttacg gtgatgatat ttcgttctga
    13261 gggggtgacg atggtacatg accgcatagc ggaggaactg gaggcgaaag gcttttaccg
    13321 gagggcggcg gcgcgatggg gtgaagtcat gcagctggtg gagacagaca aggaacggca
    13381 tcacatcacg atgcgacggc tggaatgttc aaggaaggca cagagggcac cggagccgcc
    13441 ggataatttc ggagacctga aaaaggcagt cgatcgcact tatgccgaaa tgggtataga
    13501 tggtgctggt gatgaaatat ggcgcaatta ccaggacagc taatcaaaca gccggagaaa
    13561 tccggctttt tttgcaccag ttgaaacggt atggcgcatt accgggtttt cgtcacggtc
    13621 aggcatagtt actatctgaa acaaacagac acaacagagg aaaaaaacaa tgccgatgaa
    13681 atttgatgag atattaaaac agcgtgataa ataccatgct gacaacatgg agacgatgag
    13741 catcaatgat taccgcgcat tcctggagac gggcgcactg attgaaaagg atcagcatgg
    13801 ttttgtgaga tgtgctctat ccggtgaaat gctggcggta aatcctgaac agatagatgc
    13861 attgatagaa tttctgaaag agatcagaga ctgagccagc acacagcaca catagccgga
    13921 gcaatccggc ttttttgcgc ccaaaaaaag cccgataagg tcagagggtt cttatcgggc
    13981 ttttgcatat gaggtttttg gatgcactgg cgttcgtgat cgggataatc atttcataat
    14041 ttgcaacata actcaatatc attgcataaa atgcaattct gattataatc aggactggat
    14101 aaacatccag ttatgatttt tttagtcgaa gaggaatttc ttactatggc tgaagagaaa
    14161 aaaagcggtg tttcggtgta cataagcccc gaaatcgtgg aggtgctcaa gcagcgccac
    14221 aaaaaaaact atgaagctgg cgttgcggct ggactggatc cgctgatgac gccggagccg
    14281 tcgataggtt cacttgtacg ctcttattta cttgcggcgc ttgggatgca taaaaattat
    14341 gggggtgaat aatggcaggc aaagcaacgg cacttaacac taaccagctt ttcatgtacc
    14401 tgaatcgcgg ggatattgcg gattttaaat tcagccctct gtttaccacg ctgtttttcc
    14461 cgaacgtggc gacattcagc acgcaaaaca tcatgctgga taccctggac attgaagaag
    14521 tcaccatgtc ggcgttttgt tcgcctatgg tgggtagcca ggttcagcgc gataaagggt
    14581 acgaaaccag cacaatcaaa cctggctaca tgaagccaaa gcacgaaatc gatccaacga
    14641 aaaccatcat gcgcatggct ggagaagatc cggcacagct taacgaccct acctatcgcc
    14701 gtatgcgcct gattactggc aacatgcgcc gccagataaa cgccattaag gcacgcgtgg
    14761 aatggctggc ggtgaatgcg gtaacgaccg gaaaaaacat cattgagggc gaaggcatag
    14821 aacgctatga aatcgactgg aaaataccgg aaaactgcat catagagcag gccaagggta
    14881 aaaaatggtc cgagcaggat aaagacatgc acgacccaat ctatgacatc gagctttatg
    14941 ctgatcaggc tggttgcccc gcaaacgtca tgattatggg cgctgaggta tggcgcacat
    15001 tacgcagctt taaaaaattc cgtgagctgt acgatctttc ccgtggttca gaatccgccg
    15061 ccgaactggc ctgtaaaaac ctgggcgaag tggtgagctt taaaggctat cttggtgatc
    15121 tggcccttat cgtctattcc ggcaaataca ctgacagcga tggtaccgaa aaatatttcc
    15181 ttgagcctga tttgctggtc ctgggtaaca ccaacaataa agggctggtg gcctatggtg
    15241 cgataatgga tcaggaagcg gtaagaacgg gcgcaacaca aaacatgttt tacccgaaaa
    15301 actggattga ggacggcgat ccggcgattg agtacgtgca gacacacagt gcaccgcagc
    15361 cggtaccggc agatattcgc aaatttgtta ccgtcaaaat tggttaacgg ggggattcta
    15421 tggacactcc atacattgag ttatttgcag gcagtcagca ggtatccacg acgttggtac
    15481 attttgccgc tgatgctggc gttattcagg aatttacccc gctgatgctg gcagacaatg
    15541 gcgagtttaa gccgtgggat ggtcaggaat ctggcaaggc tgtttatctg acctcgtacc
    15601 ctgtggacac gtcaaagcag aaatcagcac agtgttacaa gacggggata ttcaatattg
    15661 ccgccgttaa ctggcctgag agcgtcgaca ccgatgcgaa aaaatgcgcc gcctttgcgg
    15721 gttccggcgt atccgttcag ccgctggccc gataagcagg gggaacgatg gcaacgaatg
    15781 aaagcatcat gacgctaccg ctggcgagta aatttaaagt cgaagcgcgg gcaatggctg
    15841 acagaggttt atcaacctat gaggccgtat atcaactaaa caaactggaa gagcaggaca
    15901 agccgtgcgc tgatgcgatt atggcgcttc atgagcataa agactatcag ccgctgttgc
    15961 gtgcaatggc aaacgtgcct tgtatcgatg ttggtacggc taaaagcatc cttagcatga
    16021 ccatagagca ggaacgtccg aaggttgcgc cggaacttac cgcagccttt gaaaacttta
    16081 tggacatgca cagcccgcaa gccgtatcag ctggcatggc atacgatggc agaaaccagg
    16141 gcgatgacgg cgacatcgat cgcatactga aaaccatctg agacaaggcc ggagaaatcc
    16201 ggctttttta cgggtccttc ctggaattat ggcccgttac ggggcggcga cctcgcggat
    16261 tttcgctatt tatgagcctt ttcagggtgg tggtggtggt tttgttgttt gatctatctt
    16321 tatgaatgaa aagggaaaga tgcaagcaat acaccaacct gaagcagtaa ttaagttggt
    16381 gtattaatga aatctcatct gatgaacaaa aaaaacatgg cgaaaagctg ccgtgtaagt
    16441 gcgacagcgt tcgacaagtg gggagtaact cccgttgagc gtaaaggccg tgaggcgttt
    16501 tatgatgttg ccagcgtgat agaaaatagg gttaacaatg caattagcca gcttataaac
    16561 gacaaaggcg agattgacga tgatgaactt ttacgagtca ggatcagatt actgacagca
    16621 caggcagagg cgcaggaact taaaaacgat cgcgatcgcg gtgacgtgat tgatactgag
    16681 ttttgcctgt acgcgctttc aaagctggcg agtcagattt catcaatcat ggacagcctc
    16741 ccgcttacta tgcaaaggag cttcccacag attacccccg ccatgctgga caggcttaaa
    16801 agggaagtgg ttaaagcctg taatgccagt gccagagttg ccgacaacct cccacagata
    16861 ctggctgatt acttgaaaga aacaaccgga aacgtaccgg aaaagttgct acagaagaaa
    16921 ggcgagtaac agacgcgcaa ttattgaaca aaaatgagaa aggatctgat aatggcattt
    16981 tctggctaaa atgcctcgtg tcagatcctt tcttttttta atattttcat tgttaaacag
    17041 tctgttacag agaagaagat cggatctgac ttttgccgga aaattctcat aaacagataa
    17101 aaaccgcgag gtcgccgccc cgtaacgggc cataattcca ggaaggaccc gacgacacca
    17161 gactatcaga gcgatggggg cacaatgaca gaagccgaac tactgggatt aatccgccgc
    17221 gtcgccggaa tcagccagca gcatgacgaa caggccacac agccggacag cgtgaccgct
    17281 gaaaattatg tgcgtgttgt agcggaagtg atgcgccgtg atggtataga acttaatggc
    17341 gttgatatgc gcaacatacg gaaccgcgtt cttgaaatgc tggcctacaa tcgccgcgtt
    17401 gaactgtatc gggagaaaga aaaaataacg taccactgga agaagccgga acggctgcgg
    17461 cggtaacttg ctgatattcc cgataacgca aaattgcgct ggctacatcc tcacatctga
    17521 ggactgttaa tcagaaggcc ggaatatatt cacaaactac gaaattttcg tagtttaatt
    17581 aatggtcaga ttaatcgcta acccgctgat attttcggaa acctcaattt gaggaagtcg
    17641 gaagcgtcac ccttcatatt ctgaatggtc ataatgacca cgcagaccat caaggcaacc
    17701 acaacgaaca tcgttacggt tggagctttt accccactgg gggaaaggta ttacgataat
    17761 cataacacct accgaacagg taaaacccac cagcctgatt aacagttaac cggaaaaaaa
    17821 atccggtagc cccgttatgg ggatccccat atcgacatta acgcccctca tgaattggtg
    17881 cgcttccccc tggaaagatt acccgcctgt atatttcttg tgtctatttg ttccacgttg
    17941 tttcatacag tgcaccgaac ggtgtagtta atggtgtagt caatttgctg tttttagcac
    18001 tttttgcagt gtgggttgtt taattaaatc aatgggttgc cttgctgtgg tgataaaagg
    18061 acatatttat tcatctttcg gcgtagaatg ctggcgacca aaaatcacct ccatccgcgc
    18121 accgccaagc atgctctctc cggcgacgat tttaccctca tattgcttgg tgatttcgcg
    18181 ggctaccgcc agccccacac cttgcccagg gcgtaaagta tcaacccgtt gaccacggtc
    18241 gaaaatgacc tctcgcttgc ttaatggaat accggggcca tcatcctcga ccacaatata
    18301 gagatgctcg tcggtttgcc ttgcagaaat ttcgacaaac tcgaggcaat atttacaggc
    18361 attatccagt acattgccca tcacctcgac aaaatcgttc tgctcaccga caaagctgat
    18421 ctctggcgaa atatcgagag agatattgac ccctttgcgt tgatacactt tgttcagcgc
    18481 cgaggtgaga ttgtccagca gtggggcgac cggatgcagc tcgcggctga gcaatgtccc
    18541 gccgtgcata ctggcacgat gcaggtagta gccaatttgc tgtgaaatgc ggctgatttg
    18601 ctccagcatt accggttcag catcactgac gctcatcttt tcactacgta gagaacgcag
    18661 cgtactttgc agcaccgcca gtggcgtttt cagactatgg gtcaggtcgg tgagcgttgt
    18721 acgatatttg tcgtaacgtt cgcgttcact ttttaacaat cggttcaggt ttcgtaccag
    18781 actggtcagt tctcgcgttg tggctggatt gagcaattcg cggttatgtt cttccagttc
    18841 gcggacttct tttgccaggg cttcgatggg gcgtaaactc caccaggcgg cgacccacag
    18901 cagcgggatc actaacagca gattggctga gagcacatag ataaaccagc tccagaccat
    18961 ataggaactt tttagctcca ccggaatggt atccaccacc acaatggtta actttggcat
    19021 ccgcgatgtt gccgggtaga cgtttaccgc caccgagtgg gtcatctccg cgtcgtcatc
    19081 atcttcccgc acttcctgca actgttgctg tatcgaatga tctccactca gcaagaggct
    19141 ggtatcgtta acatccgctt caatttcatg aaaaccattc gatttcagcc agtcaggctg
    19201 gatcatcttc atcagccagg gcacgtcacg ttgcgcccat aaaagctgcc cgttctcatc
    19261 ataaattagc gtcatggtgg ggctttgctt gtcgatattt tcgggtaact cgacatgcaa
    19321 cttattgttt tcccacttcg caagggtata gaacagattg ctctcgccac gtaacagccg
    19381 aaacgtagtt ttatcgaaac tgacgctata accgatcagc gcgaccattc cgtaggcaag
    19441 cgaaagcacc agtactaccg ctgccgttgc caacagaaaa cgtacccgca gcgagagcgg
    19501 gaaaaaaaga cgcagtaatt ttttcatcag cgcaattcga acaaatagcc ctggccgcga
    19561 acggtagtaa tcacttcttg cggatattgt gcctgaattt ttttgcgcag acgtcccatc
    19621 agtacatcaa tggtatggct ttcccgcagc tccgcatccg gatagagttg tagcattaac
    19681 gaatctttgc tgaccacttt gccattattg cgtatcaacg tttccataat ggtgtattcg
    19741 aacgcggtca gtttgatcac ttcgtcatta atagataatt cacggcgaga gagatcaacc
    19801 tgaaacgggg ggagcgaaat gacctgtgaa gccaggccgc tattacgccg cattaatgcc
    19861 tgcattcgcg ccatcacctc ttcaatatga aacggtttag tcacgtaatc atcagcaccg
    19921 gcacttaata cttcgacttt gtcctgccag ctttcacggg cggttaatac cagaatcggc
    19981 agtgaaacat cattgctacg ccagcggcaa atcagtgaca gaccgtcctc gtctggcaat
    20041 ccgagatcga caatcgcaat atccggtaaa tgttcattga gataataatc ggcttctttg
    20101 gcatcttctg catcatcgac ctgatgacca gcatcctgaa tctgaacttt aaggtggtga
    20161 cgtaacaacg cattgtcttc aacaaccagt acgcgcattt
//
LOCUS       DABSYT020000079        20031 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.079, whole genome shotgun sequence.
ACCESSION   DABSYT020000079 DABSYT020000000
VERSION     DABSYT020000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.079"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(238..444)
                     /gene="ybfA"
                     /locus_tag="ID725_RS17275"
                     /old_locus_tag="pgaptmp_003455"
     CDS             complement(238..444)
                     /gene="ybfA"
                     /locus_tag="ID725_RS17275"
                     /old_locus_tag="pgaptmp_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGV7851972.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            757..846
                     /gene="kdpF"
                     /locus_tag="ID725_RS17280"
                     /old_locus_tag="pgaptmp_003456"
     CDS             757..846
                     /gene="kdpF"
                     /locus_tag="ID725_RS17280"
                     /old_locus_tag="pgaptmp_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGV7851973.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            846..2519
                     /gene="kdpA"
                     /locus_tag="ID725_RS17285"
                     /old_locus_tag="pgaptmp_003457"
     CDS             846..2519
                     /gene="kdpA"
                     /locus_tag="ID725_RS17285"
                     /old_locus_tag="pgaptmp_003457"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGV7851974.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2542..4590
                     /gene="kdpB"
                     /locus_tag="ID725_RS17290"
                     /old_locus_tag="pgaptmp_003458"
     CDS             2542..4590
                     /gene="kdpB"
                     /locus_tag="ID725_RS17290"
                     /old_locus_tag="pgaptmp_003458"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGV7851975.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4599..5171
                     /gene="kdpC"
                     /locus_tag="ID725_RS17295"
                     /old_locus_tag="pgaptmp_003459"
     CDS             4599..5171
                     /gene="kdpC"
                     /locus_tag="ID725_RS17295"
                     /old_locus_tag="pgaptmp_003459"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGV7851976.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5164..7848
                     /gene="kdpD"
                     /locus_tag="ID725_RS17300"
                     /old_locus_tag="pgaptmp_003460"
     CDS             5164..7848
                     /gene="kdpD"
                     /locus_tag="ID725_RS17300"
                     /old_locus_tag="pgaptmp_003460"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGV7851977.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7845..8522
                     /gene="kdpE"
                     /locus_tag="ID725_RS17305"
                     /old_locus_tag="pgaptmp_003461"
     CDS             7845..8522
                     /gene="kdpE"
                     /locus_tag="ID725_RS17305"
                     /old_locus_tag="pgaptmp_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGV7851978.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGYRFML"
     gene            8684..8818
                     /locus_tag="ID725_RS17310"
                     /old_locus_tag="pgaptmp_003462"
     CDS             8684..8818
                     /locus_tag="ID725_RS17310"
                     /old_locus_tag="pgaptmp_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGV7851979.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            8797..8901
                     /gene="speFL"
                     /locus_tag="ID725_RS17315"
                     /old_locus_tag="pgaptmp_003463"
     CDS             8797..8901
                     /gene="speFL"
                     /locus_tag="ID725_RS17315"
                     /old_locus_tag="pgaptmp_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGV7851980.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9212..11410
                     /gene="speF"
                     /locus_tag="ID725_RS17320"
                     /old_locus_tag="pgaptmp_003464"
     CDS             9212..11410
                     /gene="speF"
                     /locus_tag="ID725_RS17320"
                     /old_locus_tag="pgaptmp_003464"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HGV7851981.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11407..12726
                     /gene="potE"
                     /locus_tag="ID725_RS17325"
                     /old_locus_tag="pgaptmp_003465"
     CDS             11407..12726
                     /gene="potE"
                     /locus_tag="ID725_RS17325"
                     /old_locus_tag="pgaptmp_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGV7851982.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12783..14423)
                     /gene="pgm"
                     /locus_tag="ID725_RS17330"
                     /old_locus_tag="pgaptmp_003466"
     CDS             complement(12783..14423)
                     /gene="pgm"
                     /locus_tag="ID725_RS17330"
                     /old_locus_tag="pgaptmp_003466"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGV7851983.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14449..14994)
                     /gene="seqA"
                     /locus_tag="ID725_RS17335"
                     /old_locus_tag="pgaptmp_003467"
     CDS             complement(14449..14994)
                     /gene="seqA"
                     /locus_tag="ID725_RS17335"
                     /old_locus_tag="pgaptmp_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGV7851984.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15179..15943
                     /gene="ybfF"
                     /locus_tag="ID725_RS17340"
                     /old_locus_tag="pgaptmp_003468"
     CDS             15179..15943
                     /gene="ybfF"
                     /locus_tag="ID725_RS17340"
                     /old_locus_tag="pgaptmp_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGV7851985.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16083..16376
                     /gene="ybfE"
                     /locus_tag="ID725_RS17345"
                     /old_locus_tag="pgaptmp_003469"
     CDS             16083..16376
                     /gene="ybfE"
                     /locus_tag="ID725_RS17345"
                     /old_locus_tag="pgaptmp_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGV7851986.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16516..17046
                     /gene="fldA"
                     /locus_tag="ID725_RS17350"
                     /old_locus_tag="pgaptmp_003470"
     CDS             16516..17046
                     /gene="fldA"
                     /locus_tag="ID725_RS17350"
                     /old_locus_tag="pgaptmp_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGV7851987.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17256..17342
                     /gene="uof"
                     /locus_tag="ID725_RS17355"
                     /old_locus_tag="pgaptmp_003471"
     CDS             17256..17342
                     /gene="uof"
                     /locus_tag="ID725_RS17355"
                     /old_locus_tag="pgaptmp_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGV7851988.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17335..17781
                     /gene="fur"
                     /locus_tag="ID725_RS17360"
                     /old_locus_tag="pgaptmp_003472"
     CDS             17335..17781
                     /gene="fur"
                     /locus_tag="ID725_RS17360"
                     /old_locus_tag="pgaptmp_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGV7851989.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17865..18191)
                     /gene="chiQ"
                     /locus_tag="ID725_RS17365"
                     /old_locus_tag="pgaptmp_003473"
     CDS             complement(17865..18191)
                     /gene="chiQ"
                     /locus_tag="ID725_RS17365"
                     /old_locus_tag="pgaptmp_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGV7851990.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18241..19646)
                     /gene="chiP"
                     /locus_tag="ID725_RS17370"
                     /old_locus_tag="pgaptmp_003474"
                     /pseudo
     CDS             complement(18241..19646)
                     /gene="chiP"
                     /locus_tag="ID725_RS17370"
                     /old_locus_tag="pgaptmp_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         4634 a         5018 c         5306 g         5073 t
ORIGIN      
        1 ctcatccttg ttttatcggg ttatttgtct gcttccaggc aaaaccctac agatttataa
       61 gaaagcctta agtgagatat ccgtcagata gaaacaatta ggaaaatatt tatatatttc
      121 atgaatatat gtttcgattg gtataacggt gtctaaatat tcctctgaac atcttcctga
      181 tacaaaaaac cgccagcgtt tccgctggcg gttttcaatg tattcaaaac tcagcaatca
      241 gtaaaaatca ccggttgcct tttcggcctg atccatccaa accggtttat cgctggtttt
      301 cgaccagacg cgatgcaggt agctgtaaaa gcgggcgcgg tctttccaga aaagcatgaa
      361 aggcagcgcc agaacgcccg ctgcaaccgc ataagtacgg cgtaaaaaga taagccatgc
      421 aggatattct ctgtagagtt ccatactttt ctcccccttt tgtatctacc cggtgaatag
      481 caccggaaaa atgaatttgt ttatctgatg aaaatagtac cgccttttgt gtaattttac
      541 tactcatccg accacttatt tttgcttatt gatggtttat ttacattcat cctgtaatta
      601 agttacacaa aagttaaatt aatactaaac attagttaaa tcatggcttt tgccattttt
      661 atactttttt tacaccccgt ccgcagattt ttgcgaaatc tttgcagcca gaattctacc
      721 cttccggtat cacttttagg ccactggagg tgcactgtga gtgcaggcgt gatagccggc
      781 gtgttgctgg tgtttttatt actgggttat ttggtttatg ccctgatcca tgcggaggca
      841 ttctgatggc tgcgcaaggg ttcttactga ttgccacgtt tttactggta ttaatggtgc
      901 tggcgcgtcc cttaggtagc gggctagcgt ggctgattaa tgatattcct cttcctggta
      961 cgacgggcgt tgaacgcgta ctctttagcg cgcttggcgt ctctaaccat gagatgaact
     1021 ggaagcagta tctctgtgcc attctcggcc tgaacatgct ggggctggcg gtgctgtttt
     1081 ttatgttgct cggtcagcac tatctgccgc ttaatccaca gcagttgccg gggctgtcgt
     1141 gggatctggc gctgaatacc gccgtcagct ttgtcacgaa taccaactgg caatcttata
     1201 gcggtgaaac cacgttgagc tatttcagcc agatggcggg cttaacggtg caaaactttc
     1261 tttctgccgc cagcgggatt gcggtgattt ttgccctcat tcgtgcattt acccgccaga
     1321 gcatgagcac actcgggaat gcctgggtcg atctgctacg catcacgtta tgggtgctcg
     1381 tccctgtggc gttggtgatc gcactgtttt ttattcaaca aggtgcgctg caaaactttc
     1441 tgccctatca ggcagtgaat accgttgaag gagcgcaaca gctgttacca atgggtccgg
     1501 tggcttctca ggaagcgatc aagatgctcg gcactaacgg cggtggtttc tttaatgcca
     1561 actcgtcgca tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag atgctggcga
     1621 tcttcttgat cccaacagcg ctgtgctttg cctttggtga agtgacgggc gatcgccgtc
     1681 aggggcgcat gttgctgtgg gcgatgtcag tgatttttgt catctgcgta ggcgtggtga
     1741 tgtgggcaga agttcagggt aatcctcatc tgctggcgct cggcgcagac agcagcatca
     1801 atatggaagg taaagagagc cgtttcggcg tgctggtcag tagcctgttt gcggtcgtga
     1861 ctacggcggc gtcctgtggc gcggtgattg caatgcatga ttcgtttacc gctctcggtg
     1921 gcatggtgcc gatgtggctg atgcaaattg gtgaagtggt attcggcggt gtcggttctg
     1981 gtctttacgg catgatgctg tttgtcctgc tggcggtgtt tattgccggg ctgatgattg
     2041 gtcgtacacc ggaatatctg ggtaaaaaaa tcgacgtacg tgagatgaaa ctgactgcac
     2101 tggcgattct ggtcaccccg acgctggtgc tgatgggcgc ggcgctggca atgatgaccg
     2161 acgccggacg tagcgccatg ctcaaccctg gcccgcatgg ttttagcgaa gtgctgtacg
     2221 ccgtgtcatc cgccgctaac aacaacggca gcgcctttgc cggattaagc gccaactctc
     2281 cgttctggaa ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg gtgattatcc
     2341 cggtgatggc aattgccggt tcgctggtga gtaaaaagag ccaacccgcc agctccggca
     2401 cgctgccaac gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg ttgctggttg
     2461 gcgcactgac ctttatccct gccctggcgc ttggtccggt ggcggaatat ctctcctgat
     2521 gatattgagt gagcactgaa tatgagtcgt aaacaactgg cgctattcga accaacactt
     2581 gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca atggcgcaat
     2641 ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca cctgtattag catcgcgatg
     2701 gcaagcggcg cgatgcctgg caatgcgctg tttagcgtgg ccattagcgg ctggctgtgg
     2761 gtcaccgtac tgttcgctaa tttcgccgag gcgctggcag aaggccgcag taaagcgcag
     2821 gccaacagcc tgaaaggggt gaaaaaaacg gcctttgccc gcaagctgcg tgagccgaaa
     2881 tatggcgctg cggcggacaa agttcccgcc gatcaactgc gtaaaggcga tatcgtactg
     2941 gtagaagcag gcgatattat cccctgcgat ggtgaagtta ttgaaggcgg cgcatcggtc
     3001 gatgaaagcg ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg cggcgatttt
     3061 gcctccgtca ccggcggcac gcgtattctt tctgactggc tggtgattga gtgtagcgtt
     3121 aaccccggcg agacatttct ggatcggatg atcgcgatgg tggaaggcgc acagcgacgc
     3181 aaaacgccga acgagattgc cctgaccatc ctgctgattg ccctgactat cgtcttttta
     3241 ctggcaaccg ccacgctgtg gccgttttcc gcgtggggcg gtaatgcagt cagcgtaacg
     3301 gtactggtgg cgctgctggt ctgtctgatc ccaaccacta ttggcggcct gttgtcagcg
     3361 atcggcgtcg ccgggatgag tcggatgctg ggcgcgaatg tggttgccac cagcggacgt
     3421 gcagttgaag cggcaggtga cgttgacgtt ctgctgctgg ataaaaccgg caccatcaca
     3481 ctcggtaacc gtcaggcatc ggagtttatc cctgcgcagg gcgtggatga aaaaacgctg
     3541 gctgacgccg cacaactggc ttcgctggct gatgaaacgc cggaaggccg cagtattgtg
     3601 atcctcgcca agcagcgttt taacctgcgc gagcgcgatg tgcagtcgct ccatgccacc
     3661 tttgtaccgt ttactgcgca aagccggatg agcgggatca atatcgacaa ccgcatgatc
     3721 cgtaaaggtt ctgtcgatgc cattcgccgc catgttgagg ctaacggtgg tcacttccct
     3781 gccgatgttg atcaaaaagt cgatcaggtt gcgcgtcagg gggccacacc gctggtagta
     3841 gtagaaggtt ctcgtgtgct gggcgttatt gcgttgaaag atatcgtcaa aggcggcatt
     3901 aaagaacgct tcgcccaact gcgcaaaatg ggtattaaaa cggtgatgat taccggcgat
     3961 aaccgtctga ctgccgccgc gatagctgcg gaagcgggcg tcgatgattt tctcgccgaa
     4021 gcgacaccgg aggccaagct ggcattgatc cgtcagtatc aggcggaagg ccgtttggta
     4081 gcaatgaccg gcgacggcac caacgatgct ccggcgctgg cgcaggcaga tgtcgcggtg
     4141 gcgatgaact ccggtactca ggcggcgaaa gaagcgggca atatggtgga tctcgactct
     4201 aacccgacca agttgatcga ggtggtacat attggcaaac agatgctgat gacccgtggc
     4261 tcgctgacca ccttcagcat tgccaacgat gtggcgaaat acttcgccat tattccggcg
     4321 gcattcgcgg taacgtatcc acagttaaat gcgctgaaca tcatgcgcct gcattcgccc
     4381 gactccgcca ttctcagtgc ggtcattttc aacgccttga ttatcgtctt tttgattccc
     4441 ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac
     4501 ttatggattt acggtctggg tgggctgctg gtgccgttta ttggtatcaa agtcattgat
     4561 ttactactga ccgtttgcgg tctggtgtga ggtttaccat gcgtggatta cgtccggcat
     4621 tatcgacatt tatctttctg ttattgatta ctggcggtgt ttacccgctg ctgaccaccg
     4681 cgctggggca atggtggttt ccctggcagg ccaacggttc gttgatccgt gaaggtgata
     4741 cggtgcgggg ttcggcatta atcgggcaga attttaccga caacggctat tttcatggtc
     4801 gcccgtcggc aacggcagaa atgccttata atccacaggc ttctggcggg agcaatctgg
     4861 cggtcagtaa ccctgagctg gataaactaa tagccgcacg cgttgctgca ttacgggccg
     4921 ctaacccgaa tgccagcacg agcgttccgg ttgaactggt gacggcgtcg gcaagcgggc
     4981 tggacaataa catcaccccg caagcggcgg cctggcagat cccacgcgta gcgaaagcgc
     5041 gtaatctcag cgttgaacag ctcacgcaac tgatcgcaaa atacagccaa caaccactgg
     5101 tgaaatatat cggccagccg gtcgtcaaca ttgtcgaact caatctggca ctggataaac
     5161 ttgatgaata acgaaccctt acgtcccgac cccgatcgtc tgctggaaca aactgccgcg
     5221 ccgcatcggg ggaagctgaa agttttcttc ggtgcctgcg caggcgtcgg gaagacctgg
     5281 gcgatgctgg cagaagccca gcgactgcgg gcgcaagggc tggatattgt ggttggcgtg
     5341 gtagaaaccc acgggcggaa agataccgcc gccatgctgg aagggctggc tgttctgccg
     5401 ccaaaacgcc tggcgtaccg tgggcggcac atcagcgagt ttgatctcga tgccgccctc
     5461 gcccgccgtc cggcgctgat cttaatggac gaactggcgc acagtaatgc gccaggttct
     5521 cgtcatccta aacgctggca ggatatcgaa gaactgctgg aagctggcat tgatgttttc
     5581 acaaccgtca acgttcagca tctggaaagt ctgaatgatg tggtcagcgg cgtcaccgga
     5641 attcaggtac gggaaaccgt gcccgatcct tttttcgatg ccgccgacga cgtggtgctg
     5701 gtggacttgc ccccggacga tctgcgccag cggctgaaag aaggcaaagt ctatattgcc
     5761 gggcaggcgg agcgcgccat tgaacatttt ttccgcaaag gcaatctgat cgccctgcgc
     5821 aaactggcac tgcgccgtac tgccgatcgc gttgatgagc aaatgcgcgc ctggcggggg
     5881 catcctggcg aagagaaagt ctggcacacg cgcgacgcga tccttttatg catcggccat
     5941 aacaccggca gcgaaaaact ggtccgcgcg gcggcgcggc tggcgtcacg gctggggagc
     6001 gtctggcacg cggtgtatgt tgaaacccct gccctgcatc gcttaccgga gaaaaaacgt
     6061 cgggcaattc tcagcgcctt acgtctggcg caggagctgg gcgcggagac ggcaacactt
     6121 tctgatccag cggaagagaa agcggtagtg cgttatgccc gtgaacataa tctcggcaag
     6181 attattctcg gtcgcccagc ctcgcgccgc tggtggcgtc gggaaacgtt tgctgaccga
     6241 ctggcgcgca tcgcccccga tctcgatcag gtgctggtcg cgcttgatga accacccgcc
     6301 cgcacgatta acaacgcgcc ggatagccgc tcttttaaag acaagtggcg ggtacagatt
     6361 cagggatgcg tggttgccgc cgcgttatgc gccgttatca ccttaattgc catgcagtgg
     6421 ctgatggcgt ttgatgccgc caacctggtg atgctgtatc tacttggcgt ggtggtggtg
     6481 gcgctatttt atggacgctg gccttcggtg gttgctaccg tcattaatgt ggtgagtttc
     6541 gatctctttt ttatcgcccc acgcggcacg ctcgccgtct ctgatgtgca atatctgctg
     6601 accttcgcgg tgatgttaac cgtcgggctg gtgatcggga accttactgc gggtgtgcgt
     6661 tatcaggcgc gggtagcccg ttaccgcgag caacgcacac ggcacttata tgaaatgtcg
     6721 aaagctctgg cggtgggccg cagtccgcag gacatcgccg ccaccagcga acaatttatt
     6781 gcctccacgt ttcatgcccg cagtcaggtg ttgttgcccg atgacaacgg taaattgcag
     6841 ccgttaacac atccgcaagg aatgacgccg tgggacgatg ccatcgcgca gtggagtttt
     6901 gataaaggcc tgcctgcggg tgcgggcacc gacacgttac ccggcgtgcc gtaccagatt
     6961 ttgccgttaa aaagcggtga gaaaacctac gggctggtgg tggtggaacc ggggaatctg
     7021 cgtcagttga tgatcccgga acagcagcgc ctgctggaga cgtttacgct gttagtcgcc
     7081 aatgcgcttg agcggctgac gctgaccgcc agcgaagaac aggcgcggat ggcaagcgaa
     7141 cgtgaacaga tccgcaacgc cctgctggcg gcgctttcgc atgatttacg cactccgctt
     7201 acggtgctgt ttggtcaggc agaaatctta acgctcgatc tggcaagcga aggatcaccc
     7261 cacgcccgcc aggccagcga gattcgtcag catgtgctga acactacccg actggtgaat
     7321 aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa agagtggtta
     7381 acgctggaag aagtagtcgg cagcgcgctg caaatgctgg aaccgggttt atcgtcgccc
     7441 atcaatcttt ctctgccaga accgctgacc ttaatccacg ttgacgggcc actctttgaa
     7501 cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca ggccgaaatt
     7561 ggtatcgatg cccacgttga gggcgaaaat ctacaactgg atgtctggga taacggcccc
     7621 ggtcttccgc caggccagga gcagacgata tttgataagt ttgctcgcgg gaataaagag
     7681 tcggcagtac cgggggtagg gcttggactg gcaatttgtc gggcgatagt ggatgtacac
     7741 gggggcacta ttaccgcgtt caaccggccg gaaggtggtg cctgttttcg tgttacactt
     7801 ccccagcaaa ctgcccctga acttgaagaa tttcatgagg atatgtgaca aacgttctga
     7861 ttgttgaaga tgaacaggct attcgtcgct ttctgcgcac ggcgctggag ggcgacggga
     7921 tgcgcgtcta tgaggccgaa acgctgcaac gcggcttgct ggaagcggct acccgtaagc
     7981 cagatttgat tattctcgat ctcggcctgc ccgatggtga tgggattgag tttatccgcg
     8041 acctgcgcca gtggagcgcg gtgccggtga ttgtgctttc cgcacgcagc gaagagagcg
     8101 acaaaatcgc cgcgctggat gccggagcgg atgattatct aagtaagccg tttggcattg
     8161 gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca ctccgccacc gccgcgcccg
     8221 atccgctggt gaaattttcc gatgttaccg tcgatttagc cgcccgcgtg attcaccggg
     8281 gtgaggaaga ggtgcatctc accccgattg agtttcgcct gctggcagtg ctgctcaaca
     8341 acgccggaaa agtgctcacc cagcgccaac tccttaacca ggtgtggggg ccaaacgcgg
     8401 tcgaacacag tcattatttg cgtatttata tgggacatct gcgacaaaaa ctggaacagg
     8461 atcccgctcg ccccagccat ttcattactg aaaccggtat tggttatcgg tttatgcttt
     8521 gaatttttat tcaataaatt cgcctaaata gtgattaatt tattattaaa cctgcgccaa
     8581 atattattat ttggcaggtt taatttctta acgcaaatat aaaacacgaa attacaacat
     8641 ttaagtaaca aaacagcatc tagtatggcg ctattcataa ttaatgattt attatctggg
     8701 gtatgatcgt tttttgttga tcttcttcac agattatagc catttcatgg atagaataac
     8761 tctaccttca actgacacag caagaggtaa aggtaaatgg aaaataataa ccgcttaatg
     8821 cctcatataa ggcggacaac ccatatcatg aagtttgccc atcgtaatag ttttgacttt
     8881 catttcttta atgcccgcta gtcttctgac taaagggcac cccagcgtac aggtctccct
     8941 gactttaagc attacaggtt aatacctgta tccctcggtg ctcatatact gctaaccctt
     9001 ttaaactcta cataattcga gtcgcagcat atgcaacttg aagtatgacg agtatagcca
     9061 gttaccgggc tggtctgggt tattgcatct gcaaaaagca aactactgat ttatttatca
     9121 gcggtggagc tttgcttttt ttccggcgtg atcgatttct cctttgagaa attgaggacc
     9181 tgctattacc tgaaataaag agatgaacaa aatgtcagaa ttaaaaattg ccgttagtcg
     9241 ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg aatatagacg aaagtaatta
     9301 tattgacgtt gccgccatta ttttatcagt cagtgatgtt gaacgtggaa aactcgatga
     9361 aatagacgct actggctatg acattcctgt ttttattgca acggaaaatg aagaacgtat
     9421 accagcagaa tatctttcac gtatttctgg tgtcttcgaa catggagaag cacgcaaaga
     9481 attttatggt cgtcagttag aaaccgctgc cagccattat gaaactcaac tgcgcccacc
     9541 tttctttcgc gcactggtcg attatgtcaa tcagggcaac agcgcatttg actgccccgg
     9601 tcatcagggc ggcgaatttt tccgtcgcca tccggcgggg aatcagtttg tggaatactt
     9661 tggtgaggcg ctgttccgtg ccgacttgtg caacgccgac gtagcgatgg gcgatctgct
     9721 gattcacgaa ggcgcgccat gcattgcaca gcaacatgca gcaaaagtgt ttaatgccga
     9781 taaaacctac ttcgttttaa atggcacttc atcttctaac aaagtggttt taaacgccct
     9841 gctgacaccg ggtgatctgg tgctatttga ccgcaataac cataagtcta accaccacgg
     9901 ggccttgcta caggctggtg caacaccggt ttatctggaa acagcacgta acccgtatgg
     9961 ttttatcggt ggtattgatg cacactgctt cgaagaaagt tatctgcgtg aactgattac
    10021 agaagttgca ccgcaacggg caaaagaggc gcgtccgttc cgcctcgctg tgattcagct
    10081 aggcacctac gacggtacga tttataacgc ccgccaggtg gtggataaaa ttggtcatct
    10141 gtgtgactac atcctgtttg attccgcatg ggtcggctat gaacagttta ttccgatgat
    10201 ggctgactgt tcgccgctgt tgctggatct taatgagaac gatccgggta ttctggttac
    10261 gcaatccgta cataaacagc aggctggttt ttctcaaact tcacaaatcc ataaaaaaga
    10321 cagccacatc aaagggcaac agcgttatgt accgcacaaa cgcatgaaca acgcctttat
    10381 gatgcatgcc tccaccagcc cgttctatcc gctgtttgcc gcactggata tcaacgccaa
    10441 aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat tgtgtggtaa atggcattaa
    10501 tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt ccgttcatac ctgaactggt
    10561 ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt gcggttgatc tgcgcttctt
    10621 ccagtttgta ccaggtgagc actggcattc ttttgaaggc tatgcagaga atcaatactt
    10681 tgttgatccg tgcaaactgt tgctgacaac cccaggtatt gatgcacgta acggcgaata
    10741 tgaagcgttc ggtgtacccg cgacgattct tgctaacttc ctgcgcgaaa atggcgtagt
    10801 gccggaaaaa tgcgatctta actccatcct cttcctgctg actccggcag aagatatggc
    10861 caaacttcag caacttgttg ccctgctggt acgcttcgaa aaactgctgg aatctgatgc
    10921 gccgctggcg gaagtgctac cttccatcta caaacagcat gaagagcgct acgccggtta
    10981 tacccttcgt cagttgtgcc aggaaatgca tgatttgtat gcccgccaca acgtgaaaca
    11041 actgcaaaaa gagatgttcc gtaaggaaca cttcccacgc gtcagcatga atccgcaaga
    11101 agctaactac gcctatttac gcggtgaagt ggagctggtt cgtctgccgg atgcagaagg
    11161 ccgtatcgct gccgaaggtg cgcttcctta tcctccgggt gtgctgtgtg ttgttccggg
    11221 tgaaatctgg ggtggtgctg ttttgcgtta cttcagcgct ctggaagaag ggatcaacct
    11281 gctgccaggt tttgcaccgg agctgcaggg tgtctatatc gaagaacatg atggtcgtaa
    11341 gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa agcaccctgc tgaaagggga
    11401 aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac catactgacg
    11461 atggtcaaca tgatgggctc cggtatcatc atgctgccga caaagcttgc cgaagttggg
    11521 acaatctcaa ttatctcctg gctggtgaca gccgtaggct caatggcact ggcatgggca
    11581 ttcgcaaaat gcggtatgtt cagccgtaaa tcaggcggta tgggcggtta tgccgagtat
    11641 gctttcggta aatccggtaa ctttatggcg aactatacct acggcgtctc actgctgatt
    11701 gctaacgtcg cgattgctat ttcggcggtt ggttacggca ccgaactgct cggcgcgagt
    11761 ttgtcgccag tgcagattgg tcttgcgacc atcggggtgc tgtggatttg taccgtggct
    11821 aactttggtg gtgcgcgcat taccgggcaa atcagtagca ttactgtgtg gggagtcatt
    11881 attccggtcg tcggtctgtg cattattggc tggttctggt ttagcccgac gctgtacgtt
    11941 gattcctgga atccgcatca tgcaccgttc ttcagtgcgg taggttcttc catcgctatg
    12001 acgctgtggg cttttcttgg tctggagtct gcgtgtgcaa atactgatgt agtggaaaac
    12061 ccggaacgta atgtgccaat cgcggtactc ggcggtacgt taggtgcggc ggtgatttat
    12121 atcgtctcca ccaacgtgat tgccgggatt gtgccaaata tggagctggc aaattcaacg
    12181 gcaccatttg gtctggcctt cgcgcagatg ttcacgccgg aagtgggtaa agtcattatg
    12241 gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac cattgcccag
    12301 gtgtttaaat cttcatctga tgaaggctac ttccctaaaa ttttctccca tgtaaccaaa
    12361 gtggatgcac cggtgcaggg aatgttgacc attgtgatta tccagagcgg gttggcgctg
    12421 atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc tggttaacct ggccgtggtg
    12481 accaatatca ttccgtatat tctgtcaatg gcggcattag tcattattca gaaggtcgct
    12541 aatgtgcccc cctcaaaagc gaaagttgca aactttgttg cttttgttgg cgcgatgtac
    12601 agcttttatg cgctgtactc atccggggaa gaagccatgc tgtacggttc catcgtgacc
    12661 ttccttggtt ggacactgta tggtctggta tcaccacgct ttgaactgaa aaataaacac
    12721 ggttaaaaaa gcaaagtaaa aaagggcgat cttgcgaccg ccctttttct tatttaatgt
    12781 gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc aatctgcttg
    12841 cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc cgtgcctgac
    12901 ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccaat agaagcaccg
    12961 ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac cagccagggt gctggcactc
    13021 accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc agctgcctgc
    13081 aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg ttcctgcggg
    13141 ttcttaccgg tgacagcggt gatttccgcc gccagcagac acatgatgat gccgtctttg
    13201 tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct ctcttcgccg
    13261 ccaaagccga agctgccgtc aaacagacca tcaacaaacc atttgaaacc taccgggact
    13321 tctaccagtt tacggcccaa gtcgttgacc acacggtcga tcatcgcaga tgaaaccagc
    13381 gttttaccga cggcaacagc tttgccccac tgcggacgat gctggaacag gtagttgatt
    13441 gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat accgtgacgg
    13501 tcataatccg ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc cagcaggccc
    13561 gccatcgcac actcggagga gcagtccata cggatcgcgc cgtctttatc aaggtgcata
    13621 aagcggaagg tttgatcgac ctgatcgtta acgatggtca ggttgaggtt gtaatactcg
    13681 ccaatacgct tccagtattc gataccggaa ccgcccagcg gatcaacgcc aagcgtcagg
    13741 cccgctttct gaatcgccgc catatcaacg atatcggcca gcccttccac aaacggctgc
    13801 accagatcct gctctttcac atgaccggat gccatcgctt cgtcgaggga gatacgcttc
    13861 acgcctttca ggccatcggc catcagtgcg ttggctctgt cttccaccac tttagtgacg
    13921 ttggtatcag ccgggccacc atttggcgga ttgtacttga taccaccatc ttccggcggg
    13981 ttatgggacg gtgtaataac gataccgtct gccagcgggc cacctttttt attgtgaacc
    14041 aggatggcat tggaaacggc aggcgtcggg gtgaagccat tgttttcctg cacaataaca
    14101 tctacgccgt tcgctgccag cacttccaga acggaaataa atgcaggttc ggacagggcg
    14161 tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg ttcttcagca
    14221 attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc tgcactgcca
    14281 cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc tggtttcagt
    14341 acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg tgcaggttgg
    14401 cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt agatagttcc
    14461 gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt gttcgatcat
    14521 gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc ctggcacatg
    14581 tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg caaagtaaac
    14641 gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt caagagaata
    14701 tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg catattcatc
    14761 cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga tcggcttcgc
    14821 ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag caggctgtga
    14881 tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc cgatatgctt
    14941 agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt tcatcttaat
    15001 ccagtgcagt gtcttagctg aatacaagtc gccaggagta tatgaataaa tcccggcagc
    15061 ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa accttatttg ttgcagccct
    15121 gtggcacaat ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga actttgccat
    15181 gaaattgaat atccgcgcgc aaactgcaca aaaccagcac aataattctc ccatcgttct
    15241 tgtccatggt ctgtttggca gcctcgacaa ccttggcgta ctggctcgcg atctggtaaa
    15301 cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga gagatccggt
    15361 aatgaattac ccggcgatgg cgcaggatct tgttgatact ctggatgcac agcagatcga
    15421 caaagcaaca tttatcgctc actccatggg cggtaaagcg gtaatggcac ttactgcact
    15481 ggcccccgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg actatcacgt
    15541 acgccgtcat gatgagattt tctcggctat caacgcggtc agtgaatcgg acgcacaaac
    15601 tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat gaagaagggg tgattcagtt
    15661 tctgttgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat tgtgggatca
    15721 gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg ccctgtttat
    15781 ccctggtggt aattctccgt atgttagcga gcagtaccgt gatgatttac tggcacaatt
    15841 tccacaggca cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg ctgaaaaacc
    15901 ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact gattaaaaac
    15961 ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg ctgatgtatt
    16021 atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa gtcaccaaga
    16081 tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac gagcgtcgac
    16141 cgggacgacc gaaaacaaat ccgctttcgc gcgatgaaca gctgcgtatt aataaacgca
    16201 accaactaaa acgcgacaaa gtacgtggcc taaagcgtgt cgaactgaag ctgaacgcgg
    16261 aagctgtcga ggcgctgaac gagctggcgg aatcgcgcaa tatgagccgt agcgaactga
    16321 tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc gtttaaattt
    16381 ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca agactgcctg
    16441 ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa taagtttcaa
    16501 gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac cggtaatacc
    16561 gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga tgtccatgac
    16621 attgcaaaaa gcagcaaaga agatctggaa gcttatgaca ttctgctgct gggcatccca
    16681 acctggtatt acggcgaagc gcagtgtgac tgggatgact tcttcccgac tctcgaagag
    16741 attgatttca atggcaaact ggttgcgctg tttggttgtg gtgaccagga agattacgcc
    16801 gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg cggtgcaacc
    16861 atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg tctggcagat
    16921 gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact gaccgctgaa
    16981 cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga aattctcaat
    17041 gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa tgccaatcaa
    17101 aataattgct acaaatttgt aacttttgcc gttgtacctg tacaatgtcc cggtgttcaa
    17161 gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata tttgccaggg
    17221 acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc tcaagagcaa
    17281 attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt ccgcatgact
    17341 gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg tttaaaaatc
    17401 ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg cggaagattt atacaaacgt
    17461 ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact gaaccagttt
    17521 gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt atttgaactg
    17581 acacagcaac atcaccacga tcacctgatc tgcctcgact gcggcaaggt tatcgaattt
    17641 agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg cattcgcctg
    17701 actaaccaca gtctctatct ttacggtcac tgcgccgaag gcgattgccg cgaagatgag
    17761 cacgcgcacg aaggcaaata agccagtctg aacgagaaaa gccaacctgc gggttggctt
    17821 ttttatgcaa gggaaataag aaaagcgaga gttacagctc tcacttattt gttattactg
    17881 cgaatttcct gccagacctt atcacaatcg gctttcaccg cctgatcatt tccggtttgc
    17941 gttgcacgca gacactgctg ataatccagt acgcggacac tttcctggtc ggcaaactgc
    18001 tgatgttgtt tctctttctt cagcacgttt aacacgctct ggcaggcttc aattttttcc
    18061 ggcgaacctt gtgcggtgtt gatacaggcg ctgtatgcct ctttcagacg gctgtcttcc
    18121 tgtggcgcgg ttgattgcgc acaagctacc agccccgatg ccatcatggc gatgagaatc
    18181 agttttttca tagcaaattc ctcaaacgtg cggcaggcac aaaacctgcc gcgtcgggca
    18241 tcagaagatg gtgaatggtg cgattaccat aaattttacg tcacgctcat cctggaagat
    18301 gttgccgtaa ccgccgcccc agcttgggat gtcggagtgg ttgtcgtatt cggtgaagtg
    18361 cagtttgaac atcgtgcctt tggcgcgacc gtcctgaatg gtgtagaccg catccaggct
    18421 gtatgcagac tcttcaatag tccggttttt gtcgtagtac gcatccggat tgctctgcca
    18481 ggtcgcaggt ttagcatccc atgcgtaaac gtaggaagcg ccgatggcga agcctggaag
    18541 attccagttt ttcaggtcat acatcgcacc gaagaagacc gctttttcgc cgttggcgtt
    18601 gaagtcagaa cggttatccc accagatatc caggcgaccg ttcgaggaag cgtaggttgg
    18661 agtcatacgt tgcaggaagt atccctgctg accgtcagct ttaacccagg tgccttcgag
    18721 gcgcaaatcc actacgtcag ccgcccggta accaaaggtc aacgcttgca gccaggcggt
    18781 gccgtcataa agatcgttga cgctgcgatc gtcaacttta tcgcgcgtac cgtagaactg
    18841 gtagctggtg gttaacgggc taccggcgat atcaaatttg tagctggctt tggcaaagta
    18901 ttgatcgata tacccttccg cctgaccaaa tgccgcttcc agtacgaagt tatttttgaa
    18961 gtcgtatttc gccccaaggg agtgcagata atcaactttg gtggttttat cgttctgata
    19021 aaactcatcc atttccagat gccacggtgc tttgtattcg ttggtccaca tgtaggagaa
    19081 actcaacgca ccagcatcgc cgtaatcaaa attcgccccg gcctccgcac cctgataagt
    19141 acccggcata aagctccagt gcggcgctaa cagcgtttga ccagttggct gaatataacc
    19201 tgccctcgcc acaccggacc gtatttaaat ttggccgctg ctttatacag gcttataccg
    19261 cttttatcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa cgcgatttcg
    19321 ttcgggtgag agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc ggcaatatca
    19381 aggccgaaca tatcagcagc ataaccggac tgaaaatcga ggttggcgtt ccaggtggag
    19441 tgagaaaggt tggttttgta tttatcgccg tcggtaacat ctttacggtc gcgttcacgc
    19501 tgccagtaat agataccgcc ggttaaggtt gaatcgtcaa tgaatccttc ggcgcgagcc
    19561 tccggcattg ccataaagcc cgacattgct gtaacaccgg cgatagccag cgccagcgta
    19621 ctacgtttgc cactaaacgt acgcatgggt taatcctctt tgacgtataa attgctgcac
    19681 cacaggtgaa cagcgaaaaa aataaaaatt aaaaaagtag cgttgagaaa agtctcgctg
    19741 actacaggaa cagactattt ttcgcgacgc gaattatcaa tctctttctg taaccagaat
    19801 gtaagaacat gagcaaccgc acatattttg atgatttttg ttacaaggct taagtttgcg
    19861 gttgaaaggc ttgcagaaga ataaaagcgt gaaattccag caagatgaaa gcggttacat
    19921 tttatctaat tatgttttgt gtttttaaat ttattttatg gataaattgc gaagcggaat
    19981 taattcgcga agaaaagcag aaaaaacgcc gctgaacgcg gcgttttgga t
//
LOCUS       DABSYT020000080        19862 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.080, whole genome shotgun sequence.
ACCESSION   DABSYT020000080 DABSYT020000000
VERSION     DABSYT020000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.080"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..260
                     /locus_tag="ID725_RS17375"
                     /old_locus_tag="pgaptmp_003475"
     CDS             <1..260
                     /locus_tag="ID725_RS17375"
                     /old_locus_tag="pgaptmp_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7851991.1"
                     /translation="RKLMQSLCGCGKHDDCENGQSLTAQLRLGPADILESDENGIIPE
                     QDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            399..587
                     /locus_tag="ID725_RS17380"
                     /old_locus_tag="pgaptmp_003476"
     CDS             399..587
                     /locus_tag="ID725_RS17380"
                     /old_locus_tag="pgaptmp_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851992.1"
                     /translation="MPENRRIILDSKESWLIICDSQLGHLMRSMYQGRRFIQLNLEKL
                     KGVHDVALPVKWEFTRRQ"
     gene            882..2507
                     /locus_tag="ID725_RS17385"
                     /old_locus_tag="pgaptmp_003477"
     CDS             882..2507
                     /locus_tag="ID725_RS17385"
                     /old_locus_tag="pgaptmp_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851993.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNANIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPTKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPMSIVAYSWRGD
                     YIGCYTKPDGKQDVFVFSPANMDIRYLSTPFDCAWIDLAKDMMRVVTGDKMSVLAGDS
                     LPSMIRWHSKIFSLPERTSFSCIRVKSPAPERVGITVMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            2504..3772
                     /locus_tag="ID725_RS17390"
                     /old_locus_tag="pgaptmp_003478"
     CDS             2504..3772
                     /locus_tag="ID725_RS17390"
                     /old_locus_tag="pgaptmp_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGV7851994.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            3787..4065
                     /locus_tag="ID725_RS17395"
                     /old_locus_tag="pgaptmp_003479"
     CDS             3787..4065
                     /locus_tag="ID725_RS17395"
                     /old_locus_tag="pgaptmp_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851995.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            4071..4688
                     /locus_tag="ID725_RS17400"
                     /old_locus_tag="pgaptmp_003480"
     CDS             4071..4688
                     /locus_tag="ID725_RS17400"
                     /old_locus_tag="pgaptmp_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851996.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            4779..5513
                     /locus_tag="ID725_RS17405"
                     /old_locus_tag="pgaptmp_003481"
     CDS             4779..5513
                     /locus_tag="ID725_RS17405"
                     /old_locus_tag="pgaptmp_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGV7851997.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKNSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGITAGIGLKF"
     gene            5744..5884
                     /locus_tag="ID725_RS17410"
                     /old_locus_tag="pgaptmp_003482"
     CDS             5744..5884
                     /locus_tag="ID725_RS17410"
                     /old_locus_tag="pgaptmp_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGV7851998.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            5941..6342
                     /locus_tag="ID725_RS17415"
                     /old_locus_tag="pgaptmp_003483"
     CDS             5941..6342
                     /locus_tag="ID725_RS17415"
                     /old_locus_tag="pgaptmp_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7851999.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            6437..7093
                     /locus_tag="ID725_RS17420"
                     /old_locus_tag="pgaptmp_003484"
     CDS             6437..7093
                     /locus_tag="ID725_RS17420"
                     /old_locus_tag="pgaptmp_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852000.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGSGGSGGAKTPGTGANAIQYQAQNWRL
                     "
     gene            7096..7542
                     /locus_tag="ID725_RS17425"
                     /old_locus_tag="pgaptmp_003485"
     CDS             7096..7542
                     /locus_tag="ID725_RS17425"
                     /old_locus_tag="pgaptmp_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852001.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            7552..7845
                     /locus_tag="ID725_RS17430"
                     /old_locus_tag="pgaptmp_003486"
     CDS             7552..7845
                     /locus_tag="ID725_RS17430"
                     /old_locus_tag="pgaptmp_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HGV7852002.1"
                     /translation="MGYGLLDIANQSRREALQGISEADRRREEIEASNKMMAAQQKAQ
                     KKQNIATGIGTGAAVGASVAGSSAAMGATLGAWAGPVGIAAGAVIGGIAGALF"
     gene            7856..9121
                     /locus_tag="ID725_RS17435"
                     /old_locus_tag="pgaptmp_003487"
     CDS             7856..9121
                     /locus_tag="ID725_RS17435"
                     /old_locus_tag="pgaptmp_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852003.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHRKENDQRTYDLNVRNADREYALKEREHKAAQNYRNASLGLSQQRLQLEKY
                     NQRRLEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            9190..17565
                     /locus_tag="ID725_RS17440"
                     /old_locus_tag="pgaptmp_003488"
     CDS             9190..17565
                     /locus_tag="ID725_RS17440"
                     /old_locus_tag="pgaptmp_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HGV7852004.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAKATDEWLTGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEEAAMRDAETVQQDDAA
                     PQPESVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     AKRQAVMDELKAKPRPELLEEYRRLSLKEGRTETEEQQLQAIRDVLRPQQEARPEAQP
                     ENTDDGDGSIYPTVRFRDPDEVRIEINGSGASRPAERIEKVRPDNRYFTDEKSAMGSD
                     VFRNASATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERITDADPMEQP
                     AFMRDPRFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQELTERPRLPA
                     PGDIHPGQGYPLPGEVARTPDENQAGRGGRFTITGEVKGQSFQKGRAPAPENAAGRQG
                     ETLEGETVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELAQ
                     QAEVRRQARENRDILQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGAAK
                     HRYAQLAETLGKTLEDRDYVTQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIPA
                     KTKLNAVKWVESVKSIRTSVAGMLEGKVTAGRLAEWMEGRMPARYADTWQLLRTLPPS
                     QIDKASAYRVVSGVYQAAGGKRYDPPRKLYSLRNKDNKGSNLFFSESRDELLTKAKAW
                     FAEQEEKSQAKGDEKTALSPDDKISFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASDA
                     RKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPKQFSDAFGFRGV
                     QFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAHY
                     EPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDFMTEARRVKRVF
                     KDGRFVDAEYPVRQEVYDAFKGVIEAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARA
                     FERYVQDKARKAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRTLK
                     TRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDL
                     KVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEII
                     HHAMEHVVTPAEYQTIIKTVLKTRDSDNVTIREAWRKVDASYGKESPEVQAGEFLAHM
                     AEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPR
                     EDGAGASSQYSRSGKRDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVLRH
                     LGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLL
                     DAVDKNGDPVVSAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKLSR
                     DNLLHRGLQLPKGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQD
                     KFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADY
                     KIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQY
                     DRLAGIIDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGK
                     GFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDY
                     WQVFTDDRPDTMRTIAERKDQETGETIREVVERPVPMAMMADRYFTTKKNGKTYYIKL
                     HDPRLMRAMKNMGPETSNAFVRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNL
                     KAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVE
                     DGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALR
                     LSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRT
                     LGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVP
                     SHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNV
                     VGGVLGAFSPVGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGT
                     PKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRSGAVDVTPESLKYWIDYISGGTGRF
                     ISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSL
                     TGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVE
                     RDMKIAVDRFNREYNKKVGVE"
     gene            complement(17787..18719)
                     /locus_tag="ID725_RS17445"
                     /old_locus_tag="pgaptmp_003489"
     CDS             complement(17787..18719)
                     /locus_tag="ID725_RS17445"
                     /old_locus_tag="pgaptmp_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGV7852005.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            19013..19768
                     /gene="mlaA"
                     /locus_tag="ID725_RS17450"
                     /old_locus_tag="pgaptmp_003490"
     CDS             19013..19768
                     /gene="mlaA"
                     /locus_tag="ID725_RS17450"
                     /old_locus_tag="pgaptmp_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGV7852006.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         5111 a         4434 c         5738 g         4579 t
ORIGIN      
        1 tgagaaagct tatgcagagt ctgtgcggtt gcggaaagca tgatgactgt gaaaacgggc
       61 agtcgcttac agcacaactg cgactgggac cggcagacat tctggagtca gatgagaatg
      121 gcattattcc ggagcaggac agggtaatca cacaggtggt gatactggat gcagataaaa
      181 agcagataca gtgtgtggta agaccgctgc aaatcctgcg tgctgacggg acgtgggaaa
      241 atattggcgg gatgaaatag ccgacagctt cacaaaaacc ggagcccggc tccggttttt
      301 gttgtcatgt ataggggggg ttattagaga gtgaagtaat aaacatgtta atacgatgga
      361 gtgaaggatg ccgtgtaatt ctggttcaag agttttttat gccggaaaat cgcaggatta
      421 ttctggatag taaagaatcc tggttaataa tctgtgatag tcagttgggc catttaatgc
      481 gcagtatgta tcagggacgc cgttttattc agctgaatct ggaaaaattg aaaggggtac
      541 atgatgtcgc cttgccagtg aaatgggaat tcacacgaag acagtgaata gctttctgta
      601 tacgggaatg gcgaaaaatg gactgtatgg tgtgagtgtg aaacatcttg cgtgtgcgga
      661 gtgatgcttc tcgttgctac agcggcaatg ataatgcagt gaaaaaaggg gagcaatatg
      721 ctcccccaaa ccgaaagaaa attgcaataa tcaatgaagt tatttagtca tcatcagaat
      781 gtcatgcaag gcattttgtt tcagtgatgc cgatcgcgat tttagcgaat tccatcataa
      841 atcccctgat ttttaagcct gaagcagtca aaggaatttc tatgccctat atcgatatca
      901 ccacgatgcg tgggatgatg ccgcgcgttg tgacatccat gctgcccgag cattccgctg
      961 tactggcgga ggactgccat ttccggtttg gtgttattac accagaacgt cagatatccg
     1021 gggttgagaa aacattcaca attaagccaa aaacaatttt tcattaccgt gacgatttct
     1081 ggtttgcatg gccggatgtg gtggatgtga tccgcagtcc gatcgctcag gacccccacg
     1141 ggcgtattta ctacactgac gggcgttttc ctaaagtgac ggatgcgact attgccacaa
     1201 aaggggacgg gaatcacccg acatcatcgt atcgtctggg gatccccgcg ccgacgacag
     1261 ctcctgtctg tactgttcag cagggcggtg atgtttctga cgataacccg aatgatgacg
     1321 aaacccggtt ttatactgaa acctttgtct cagattatgg tgaagaaggt ccgcctggtc
     1381 cggcgtctct ggaggtaaca ctccgtactc cggggactgc ggtacaactg acgctggctc
     1441 cggtgccatt gcagaatgcc aatattaaac gtcgccggat ttatcgctct gcatcaggtg
     1501 gaggagaagc ggatttttta cttgtggctg aactggatgc gtcagtgctc agttacacgg
     1561 acaaaatacc gacgaaaaac cttgggcctt ccctggcaac atgggattac ctgccgccac
     1621 cggagaatat gacgggtctt tgcctgatgg ctaatggtat tgctgccggg tttgccggta
     1681 atgaagtgat gttttcggaa gcgtatctgc cgtatgcatg gccggaagtg aatcgtcaca
     1741 cgacggcaga agatattgtg gctatctgtc cgctgggaac gtcactggtg gtggcgacaa
     1801 agggggagcc ttatctgttc agtggtgtat cgccttccac aatttctggc tccagaattc
     1861 cttccatgca ggcatgcctg agccgaagaa gcatggtggc gatggaggga ttcgtactgt
     1921 atgccgggac aaacggtctg gtatctgttg atgtaaacgg taatacagca ctggcaacgg
     1981 aaaagattat ttcaccagaa cagtggcaga gtcagtttaa cccgatgtcc attgtggctt
     2041 attcctggcg tggtgactat atcggttgtt acacaaaacc ggatggtaag caggatgtgt
     2101 ttgtattcag tccggcgaac atggatatcc gttatctcag cacgccgttt gactgtgcat
     2161 ggattgatct tgcaaaagat atgatgcgcg tggtgacagg ggacaaaatg tcagtgcttg
     2221 ccggggactc tctgccgtcc atgataaggt ggcattcaaa aattttttca ttacctgaaa
     2281 gaacctcttt ttcctgtatc agagtgaaat ctccggcacc tgagcgggtg gggatcactg
     2341 ttatggctga tgatgttcct gtgattcatt ttgcgccggg tacgtttaag ggaagtgtgg
     2401 tgagacttcc ggcagcaacc gggcaaaact ggcaggtgat ggtatccgga ttcgggcagg
     2461 tggaacgaat aaccctgagt acatcgatgt cggagatgcc ggtatgacca gaaaaccgtg
     2521 gcgtgcgggg aaggatttat ccacagttgt ggagaacatg gaaattggca ccgggcagcg
     2581 tggtgacgga cgccacgcat ttgtgacccg tgaggaactg gttggtctta aactcgcccg
     2641 gcgtcgaaca tcgggtggtg cctcatatgc actgaatccg ggtattgaga ttgacagtac
     2701 tttaatgact gttgattttc ccacaaaacc gctgaatttt aaggcgacag gaggatttgg
     2761 ctcggttctt cttgaatggg atatgcctaa ttatcgcgga cattcactga ctgaaatctg
     2821 gcggggtacg gaggatgacc ttgctgatgc agtgctggtt gccacgacgc cggggcaggt
     2881 ttacggcgat ccggttgacc ctggctggtc gggattttac tggatacgtt ttgttaacgc
     2941 ggcaggagtg aaaggtccat ggaatgctga aaaaggcact caggcacaaa cacagatcgg
     3001 cgtgaaggcc atcattgacc agatccgcga tgaggctgca aagtcgccgg ttgtgtccga
     3061 gctgcgtaaa gaaataaaaa acgcgcaggg gcaggctgta aaggatgctg caattaagac
     3121 aaccgaagtt gtggggactc tcagggaaga aacgacaaga acgattggtg gtattgaaac
     3181 ccgcattagc acactggatt cgtcaaccag tgaatcgctt aatgaggtcg acaagcgcat
     3241 cactaaactg gataaagaag gcggtgaggc gtttctggca atgtggtcaa aaaaagcggg
     3301 agttgatggt atcactgcgg ggatcgggat tgtcgccgga aaagacagtg aaggcaggcc
     3361 tgtaagtcag gttgcaattt ctgcgtcgca gttgtttgtc tttgacccga acaacccgga
     3421 taacaccgcc tatccgtttg cggtatcagg tggcaaggtt gtgatcccga aagcgatgat
     3481 ttatgacgcg gtgattgaaa cactggtgtc gcggaaggtt gtggcggatg aggtaaaagc
     3541 cggggtaagt atcacttcgc cagttatccg gagtgccgtt attcagaacg gaaactttca
     3601 ggttgattct cagggtaacc tgaatattgg aggccttttc agtgttacgt cacaagggca
     3661 actgacaatt cgttactcta atcagaatgt aggactggtg atccgcaatg ataaaattga
     3721 ggtttatgat cagaatggac gactggctgt tcgcataggc agattacgct gatcaggagg
     3781 tgagtattgg aatacggttt tgccatttat aacagaaata acgttaatgt tacgggcgtg
     3841 ctgactccgg tatttttcct ggacagattt acagcggagt ctggctcaaa gacgtacacg
     3901 aataaacccg acgggaaatc attgcaggct gtatgttgtt tatttccctg gaataatgta
     3961 tttgcggatc ggaaagtacc gaagataacc attaatggca atacggtgac gtggtcgaat
     4021 cttgagcagg gtatgggatc ttatatttat acattctggg gataagtgtc atgtatggtt
     4081 tgagcattat gaagccggat ggcagcgtat ggataagtcc aggttttacg ccgcagtgtc
     4141 tgatcaacaa aggcaccata ccggcgactg aaaagtcttt ttttaaaaca tcaatcccgt
     4201 caggcaaaag ttgttttttc tttatcagaa cagagaagaa ggccgatgtc atgtacacgc
     4261 atgaacagat tgatggatat catgcactaa ggcttcatgt aattgtcagg ggaacgaacc
     4321 ctggtgttac gacggtttat gctttcgcga atatggttac tccaccttct gagtatggta
     4381 tcgccatgta taacccggac ggtgagatga tttatcatgg cgaaatgatg ctgcttgacg
     4441 cgaagttaat acctgttgat atcaaatttg aaaaggacct tggatatcca tgcgcaatca
     4501 tgcctgcact ggtcgggtat tataactgga aaagaactcc ttatgatcga ccgatttata
     4561 ccacatccac tggtgctaca ggaaataaaa tatattcctg tgagcattat tccggcggtg
     4621 caacatggga tattcgaaag ccgtatatag ataaggtctt ggttattaat acatcagtat
     4681 atgattagtt gaagcgagtc tttaatattc atttaaaatg tctaaaaaga tgtattatta
     4741 aaaagtttag cgtgttatct gaatacagga tatcttaaat gaagagtata gcaacactgg
     4801 ttgtgtgtgc aatctccggg attgcctgtg taaatttatc tgcacatgca gcagaaggaa
     4861 accatacaat ttctctgggg tatgcgcact ttcagtttcc gggactgaag gattttgtaa
     4921 aggatgcgac tgctcataac agggagactt tcagtcattt cgtcaacaga aactactttt
     4981 cttcattggg cgaatataca gatggtcggg tcagtggata tgaaggcaag gataaaaatc
     5041 cacagggcat taatatcagg tatcgctacg agataacgga tgattttggc gttatcacct
     5101 cttttacatg gacgcgttct ctcactaact cacagacatt tattgatgtg cagtcagccg
     5161 atcataccag gaagattaag aatccggcag cttctgccag aacggatatc agggcgaatt
     5221 actggagtct gttagcgggg ccttcatggc gggttaatca gtacatgagc ttatatgcga
     5281 tggcagggat gggcgttgct aaagttagcg ctgacctgaa aattaaggac aatattaaca
     5341 gtagtggcgg attttctgaa agcaacagca cgaaaaaaaa ctcccttgcg tgggctgcag
     5401 gtgcacagtt taacctgaat gagagtgtta cactggatgt ggcttacgaa ggttccggct
     5461 ctggcgactg gcgcacgagt ggaattactg ctggcattgg cctgaaattc taacctgtat
     5521 ccggtaaccg tttactaccc gctgtgatgg cgggtttttt attgcccgta cagggcaaaa
     5581 accgtaaatt atgcgtgggt gcctttcggc tgatggctgg agggtgaacc tgaaggcctg
     5641 atgtggaaag gccccgagtc aacttaacgt taacccgagg ccctaacact tcgtaccttg
     5701 acaagtagaa ggttagcgcc tctccactca ggaggcaagc gctatgtcgc aaaaatcgct
     5761 tatcaccgtc acaatttgta tgacggttat cttcaccatc tggatgttac acggctcgtt
     5821 gtgtgagttc cggctgaatt tgtggggagc ggagtttgcg gcgttcttac agtgtaagca
     5881 gtaggaaaac cgcggcgggg acgaaagtcc ccgtcaactg gttgctgagg ttcagccgat
     5941 atggcacccg tttcaggtga gagaatgaac gataaaattc tccggtatat gcagcgtgtg
     6001 gtgagaaatt cccgcaaccc tgaatttatg aatgaagtta aagacgcctg ccttaaaaag
     6061 caggcgtttt gttttgaggc acctgatggc tttctggtgc tgcgttctgt gctcagtgct
     6121 gatggtatcc cttatgttct ggtgttgctg ggcgtgtgta cggggagtaa cagtgttgag
     6181 cgttatctgc cggaggtgaa gacattaacc cgtctggctg gcggacgttg ggctgaattt
     6241 catacggcaa ggcggggatt tatccggctg ggaaaacgtc tgggctttga gcgaatgccg
     6301 gatgatgagg atggcttcat ggtgttcagg atagcggtct gactgccaca gttttcatca
     6361 tcgtgtttaa accaacattg caattcacat tctgaccctg ctccggcagg gtttttttat
     6421 tatccagggg gccattatgg gtggaagtaa aggtggtggt gataccaaag taaaaccaac
     6481 agcagcgcaa atagcacagg aagaagtggc ctggaaaggg tggcaggatt acaaaaatat
     6541 cctccgcccg gctgaagata acttcatgga aaaggtcgat gacctgaaca gtgagcagca
     6601 gtacgacaat attgctggca ccacaaatct ggggtatcag aaacagtttg gcgaagcacg
     6661 gaaggagctg gcgggtaatc ttgctcagtc cggtgttgac ccgtccagtg gtcgctttaa
     6721 cgcggtaatg aatgcgaacc agagtgatca ggtaaccggg cagattgaca caaccacacg
     6781 ggggcaggta tcgcaggcag ataagtatgt tgccgggcta caggatgttg cagctctcgg
     6841 ttcaggtcag aaggcggatg cgttacagag ttttaactcg ctggcagaca gcagtctggc
     6901 aaaagctaaa tcggacgcac aggctgcgtt tacgaaacag caggggcgag cctctcttgt
     6961 tggcgctggt ttgggtgcgg taggtgcata tgcgatgcat aaggctggtg gtagcggagg
     7021 aagtggcggt gctaaaacac ctggcaccgg cgctaatgcc attcagtatc aggctcagaa
     7081 ctggagactg tgattatgga gtacggtaaa tacgaaacac ttgcgagata cggttatacc
     7141 ggagcagccc gccctcaggg ggactggcag acatccgcag cgctgacccg ccagcaatac
     7201 gacgactggc gcaccagata tttaccccgt gtagcaaggc tggctgacct tggcgagaac
     7261 aacagcctga tgaatgcaca gcttgcccgg gtgggcggcc ttgccacttc cagtctccgt
     7321 acagcgcaga tggcgcagga taaccagatg gcgagatacg gggtaagccg cccggataat
     7381 cccgacagta atacgctggg gttacgtaat gccctggcaa ttgctggcgc gaaaaatggt
     7441 atccgtgaag ccgaacagga tcgccagatg aatattctga cgggggcttc tgcaccggca
     7501 agacagaaac tgagtgttgg cggacaactg gtggcagcgt aaggaggaaa tatgggatac
     7561 ggtttactgg atattgcaaa tcagtcgcgg cgtgaggcat tacagggaat aagcgaggca
     7621 gacagacgac gtgaagaaat tgaggcgtca aacaagatga tggcggcgca gcagaaagct
     7681 cagaaaaaac agaatatcgc cacgggcatt ggtacggggg ctgctgtggg tgcatctgtg
     7741 gcaggctcca gcgctgcaat gggggccacg ctcggagcat gggctggtcc cgtcgggata
     7801 gctgctggtg ctgtgattgg cggcattgca ggcgctttgt tttaaggagt ggtgaatgag
     7861 cggatttgca caggggttac ttgccggatt cagcaccgtt gaccaggcaa tgacccgtcg
     7921 taaggagctt ggtttgcgag aagcacagct tgctcagcaa cagaaaaata acgagcgcga
     7981 ttttgaattt gcgcagtcgc agtttgagca ccgaaaagag aatgatcagc gtacatatga
     8041 tcttaatgtc aggaatgctg accgtgaata tgcgctgaaa gagagggaac acaaagcagc
     8101 gcagaattat cgaaatgcgt cacttgggct atctcagcag agactgcaac tggaaaaata
     8161 caaccagcga cggcttgagt ataacgatat gattgcccat agccagccac taatggaagc
     8221 gcttggaaaa gcaattgagg ctggcgatca ggaggctgca acgcgtctgt tcgggcagct
     8281 gccaaaggga catccattaa ttcttatgtc aaacgaaggc tatgcagcga aagcgggtca
     8341 ggccgtgatc aacctgcaaa aaatctttgg tgataagccg gacatggcga tcgattcgct
     8401 gaataccccg gaaaatctcg atgtgctttc cggcgtgttt gccccggaac tacaacagcg
     8461 tattggcatg cctgattcaa ccggggaaaa aacgataaaa gaggccagga ttggcagtat
     8521 cgtaccggcg cagcaggaag ggtacgtact tattggcctt gatctcacat acagcgatgg
     8581 ctccaccgcg cataaacctg taacagaata cggcagtgcg caccctgatg atcaaaccgt
     8641 gctggcgata cccgttgata aggctatcgc tcaggtcagg gatcgcagca aatttgcaga
     8701 gatatcgaaa aattatggtt attttatgcc gaagcagcag ggactttctc tgaaagagct
     8761 tcagaagggg gccagcaacg tagcggcgga cgcgatcaag aatggcggta atgcacaggc
     8821 tgcggtggat gaatattatg ctgcgactgg ttcacaaccg catcaacaga aaattcagca
     8881 acaaaaactt cagcaacagg ttatcaactg ggcgggagat gatcctgata agctgtcatt
     8941 tgccagaaat gtagcggccc gtcagcctga aatgctggag cctcagaatc agaaattgct
     9001 ggagaacggg tatgcgaatt ttctccgtat tcaaaaggct aggggggaac aggccagaga
     9061 tgaaagtgct tcatctgcat ctcagtttat ccgtggactg aaacagaatt acgcccagta
     9121 attcacgata ttccattaat accatttcct gatgcccggc cattgtgccg gtttttttat
     9181 ggagtctgta tggcctattc agaggaacag cgtcctgagg cgcaactcgg taaccagaat
     9241 cgtaacagcc tgaacattca gcaacccggc gaaactgaca gctatgaagc atttttctct
     9301 gatccgaatc gctggaagga taacagtacg tcgttcagcc tgggcgatgt attgccaaca
     9361 atgggtaaag gtttcgccca gtccgtccgg ggaacagggg aaatggcccg tggactcggt
     9421 gatgcgatga ttcagagccc ggtaaaaaca ggggcgcgta ttttaaatga gttcagccgt
     9481 atggggctgc cgggtgtcgc aactgtgcag gatatttttg ccggtggcag caggggggct
     9541 gatgaggtca tcgataccct gcctgatggc aaaaacgcgg ttactgatac tgtcggtaaa
     9601 ggtctgaagg caaccggtaa ggctgtcagt gatggtgcta aagccactga tgaatggctg
     9661 accggtaaga tgtcgccggg tgcagttcgt gcgctgaata cgccgatgac cgaaggctat
     9721 gatgattctg cggtctgggt ggcgaagggt gtaaacctga ttggtgcgct tgtacctgat
     9781 atggttgctg gcggtgtggc taaaaaggtg ggtgatgtca cactgcgaaa aatgctgacc
     9841 gccgggctgg agaaaaaata catcgcggca gggatgcagc cggaaagagc cacggcactg
     9901 gcagcagaag ctgtcgataa aaaaatgccg gatttattcc aggcgggcct gatcacccat
     9961 tccacagcca gtgcacaggg gcagagtgca atggcggcag cagatgctgt tcttaatgcg
    10021 gattactctg agctggcgca gtcaccgaaa tttcagcaga cgtttttgtc cattgacgcc
    10081 gacccgcagc acgcacagct tactgatcgc cagaaaatgg atctggcaaa agagcgtgtt
    10141 gccgatgagg tgcgcgcgca gctggcaacc gatcctgaat tgctggctgt gaatgccatg
    10201 gcggcaaaac tgggtgacgc acaactgttt aatctggtga cacgaggcac agcgaagacc
    10261 gttaaaagcg gcattgtcag aaatgccacg gaacaggggg cgattaatgc ggcgcagggc
    10321 ggctattcac gctatcagga aaacacggca ttgcgtgaga ccgccggaat ggatgtgtca
    10381 ccgtgggagg gcgtggctga cgcaacgatc gaaggtgcag cctttggtgc tgcgatgggg
    10441 gctccatccg gtgcggttgc cggatatcgt ggcagacgtc aggccgcaga agaagcagcc
    10501 atgcgtgatg ctgaaaccgt gcagcaggac gacgcagccc cgcaaccaga atctgttgat
    10561 ccggtggcgc agcatcgtga atccatgcag gggatgaatc gcgagcagct tctggagcag
    10621 tatgctgatg cggatatggc aacagagggt gacgcatccg cagctcatcg ccgggaagct
    10681 gccagccagc tgttgaatga actggatgaa caggcgaagc gacaggcggt gatggatgag
    10741 ctgaaggcga agccgcgtcc tgaactgctt gaggaatacc gcagactcag cctgaaggaa
    10801 gggcgtactg agactgaaga gcagcagtta caggctatcc gtgatgtgct tcgccctcag
    10861 caggaggcca gaccggaggc acagccagaa aatacagatg atggcgacgg gagtatttat
    10921 ccgacggtgc ggttccgcga tccggatgaa gtgcgtattg aaattaacgg gagtggcgcg
    10981 tccagaccag cggaacgcat tgaaaaggtg cgtccggaca accgttattt cacggatgag
    11041 aaaagcgcta tgggtagcga tgtttttcgc aatgccagcg ccaccggcct gaaaccgtcc
    11101 gtagtgaaga aaggtgagaa tcagtatgcc gttgaaatgg ataatcctgc gttctctgaa
    11161 gatgtggcaa cggaaaccat taacaccctg gctgacggag agcgtattac tgatgctgat
    11221 ccgatggagc agccagcgtt catgcgtgac ccgcgattcc ggggttttac gggagatgat
    11281 acagaagtgc aggcccgcct tgcccgtggc aacgtgccga cggcggaaga gctggtgcgt
    11341 tcacagatgg ctgaaggtga tgccggtccg acagctcagg agttaactga gcgtccgcgt
    11401 ctgcccgccc ccggcgatat tcatcccgga cagggatatc cgttaccggg agaagtggcg
    11461 cgtacgccgg atgaaaatca ggccggacgt ggtggtcgtt ttaccataac cggtgaggtt
    11521 aagggccaga gtttccagaa aggacgtgct ccggcaccgg aaaacgccgc tggtcgccag
    11581 ggggaaacac tcgagggtga aacggttcgt cggggtctgc cgtcaccgga tgcgcagaac
    11641 gcgacagcac cggtacgtga agggctaccg gctcctgaca ttgcgcgtaa tgttcgtatg
    11701 cctcagcctg aatcacttcc ccgcactgta cgggactcac tgcctgagct cgcacagcag
    11761 gcagaagtac gtcggcaggc cagagaaaat cgtgacatcc tgcagcctga gacaatcgca
    11821 cctgaatctg aaacaacagt ctctactgac agggaagcta ccgtgcgcgg aggtgaagtc
    11881 aggggcaaaa aaattgaaga ctttggtgaa gaaattaagg gcgcggcaaa acaccgttat
    11941 gcacagcttg ccgaaacact gggtaaaacg ctggaagaca gggattatgt cacgcaaccg
    12001 ctgagcaaac tgttcccgaa accggactac gcaaaactgg cgaacgaagg tgccgatgct
    12061 gataccctgg caatgatagc gctgtatcgt agcgatattc cggcgaagac gaaactcaat
    12121 gcagtgaagt gggtggagag tgtaaagagt atccgcacaa gtgtggcggg aatgctggaa
    12181 ggaaaagtga ctgccggacg gcttgctgag tggatggaag gcagaatgcc cgcccgttac
    12241 gcggacacat ggcaactgtt acgcacgctg ccaccctcgc agatagacaa ggcttctgct
    12301 tatcgggtgg tatccggtgt gtatcaggcg gcaggaggga aacgttacga tccgccacgg
    12361 aaactttatt cactgcgcaa taaggacaat aaggggagca atctcttttt ctcggaaagc
    12421 agggatgaat tactgacaaa ggcaaaagcc tggtttgcag agcaggagga aaaatcacag
    12481 gcgaaaggtg atgaaaaaac agcactgtca ccggatgaca aaatcagctt tgacgtatac
    12541 cggaataccc gcagtggcga tatttttatt gcttacggca aaaacaaaat gcgggtgaga
    12601 ggtggcttta agtcagccag tgatgcgcgt aagtacattg attcacatcg tgatgagctt
    12661 gttcgtcatg tgaaggagat gcgtgagatt tcgcgtgagg agcagcgtaa cgccaccaac
    12721 cgcgatcgta ccgggccaga acgccgcaag ggtgatgtct caccgaagca gttcagcgat
    12781 gcgtttggtt ttcgtggtgt gcagtttggt aactacgtgg aaggtccgcg tcgtcaggct
    12841 gatttgaacc gggcttatga ctcgctgcat gaccttgcgg aagtactgaa tgtaccgaca
    12901 aaagcgcttt ccctgaacgg tcgtcttggc ctggcatttg gtgcccgtgg taagggtaag
    12961 gcggcagcac actatgagcc gggtgaggtg gcaatcaacc tgacaaaagg taacggaccg
    13021 ggtgcgctgg cgcacgaatg gttccattca ctggataatt attttggtcg ttatgatgtt
    13081 tccactgacg ggaagattac gtcaggtggt gactttatga cggaagccag gcgtgtcaag
    13141 cgcgtattta aagacggccg gtttgttgac gctgaatatc cggtgcgtca ggaggtttac
    13201 gacgcgttta aaggtgtcat tgaggccatt aaaaacagtg acatgccgcg tcgttcagcg
    13261 cttctcgatg aggtgcgctc aaaaccgtac tggtcaacgg atgttgaaat ggcggcacgt
    13321 gcctttgaac gttatgttca ggataaagcg cgtaaagctg gcgtggagaa tgattatctg
    13381 gtcaatatcc gtaaggcacc tgagcacaac acagataaca cctacgctta tccgacgaat
    13441 gcggaactgg atggcggtat tcgtgaggca ttcgatcacc tgttccgcac cctgaaaacc
    13501 cgtgagacgg acaagggcgt tgcgttttat tcccgtaagg gcgttacccg cacacctgaa
    13561 ggtaatctca tttcggatgt taaccgtagt gcggaagcca aaggcagccc ggtcccgcag
    13621 gttgaagcgg ttgcccgtgg cgtgatgagc ggcattaagg acagtgacct gaaggtccgt
    13681 gtggtgaagt cacagaaaga ggctgaagcg ctggcgggtg aattgttcga tggttacggc
    13741 agggtgcacg cattctatcg tccggataaa cgagaaattg tcctggtggc ggataacatc
    13801 cctgacgggc ggaccgttcg cgagaagctg cgtcacgaga tcattcacca tgccatggag
    13861 catgttgtca caccagcgga atatcagacg attatcaaaa ccgtgctgaa aacccgcgac
    13921 agtgataacg tcaccatccg tgaagcctgg cgtaaggttg atgcttccta tggtaaggaa
    13981 tcaccggaag tacaggcggg tgaatttctg gcacatatgg cggagaaaca gccgaataaa
    14041 ttcgtggcgg catgggagcg tgttgttgcc ctggtcaaag gggtactgcg tcgtacgggg
    14101 ttactgaagc cgacggaact gaacgatatc agacttgttc gcgagaccat ccgtacgtta
    14161 ggccagcgtg tgcgggaagg ttacacgccg cgtgaggatg gcgcgggcgc atcgtctcag
    14221 tactcccgta gtggtaaacg tgatccgttc aaagtgccgg aaggtgaggg cgagcgttat
    14281 cgtgatgacc ttgccagaat gatgaaatct ctgcgcacca cagatttaac ggtaaacatc
    14341 gggcgtacgc cgccggtatt gcgtcacctt ggtgcaccgg atttgccgct ggttatttcc
    14401 cgcgatactg tgcggaaggc caccaatggt gtgaaacatg tggtgccgat ggatgttatc
    14461 gagagactac cggaactgat gcacgatccg gatgcaattt accgctcagc gacagaaaga
    14521 aatgcggttg tgatgctgct tgatgccgtg gataaaaatg gtgatccggt ggtgtcagcg
    14581 gtacacatga aggctgtccg gtcgcgtctg gaaatcaaca aggtagcttc tgtttacggt
    14641 acagaaaatg gaaaaaaact gaagagtatg gaaatgaccg ggttaacgtt gtaccggaga
    14701 gaaaaattaa gccgcgataa ccttctgcac agagggctcc aattgcccaa aggggaacat
    14761 tcttatcgcg gctctgcgga taaaatactc tatcctgaag atattcgcaa ggggccgtat
    14821 tactcccgta ccagcagtct gacaccggaa gagacaattg catcgcgttt tgtgcgccag
    14881 atgcaggata aattccaggt gctgaaagct gttcaggaga atatccgtaa aactggcggc
    14941 aaagtggacg acagtaacaa cgcttatatg gcggaagaac tcttccacgg gaaggcggaa
    15001 aacgacctga acgtgatgaa ggagcgctac gttcagccac tggctaaatt actggcggac
    15061 tacaaaattg cgcaggccga tctggatgag tacctctacg cccgtcacgc gccggaacgt
    15121 aacgcgcata tcgcgaaaat caacccgaaa atgccggacg gcggttcggg gatgaccaac
    15181 gcggaagcgg cggaaatcat gcagcgtgta cgtaacagtg gcaaacaggc acagtatgac
    15241 cgtctggcag ggattattga cgatatgctg gcccgtcgcc gtgagcttat ccgtgaggcc
    15301 ggacttgaag agaacggtgt ggtggatgcc tggcagaacg cctaccgtta ctacgttccc
    15361 ctgaaaggtc aggatgttga cggtgtggtg tcactgcccc gtacaggtaa gggcttcacc
    15421 atcggcggac gtgaaagcag gcaagccatg gggcgtgcat cccgggcaca gtctccgtcc
    15481 actcaggcga tacaggacct gagcgaatcg ctgatccgcc atcgcaaaaa cgaagtgggt
    15541 aacgccttcc tgaaactggt gcaggataat cccgacaagg attactggca ggtattcacc
    15601 gatgacagac cggataccat gcggacgatt gcagagcgca aggaccagga aactggtgaa
    15661 accattcgcg aagttgtcga acgccctgta ccgatggcaa tgatggcaga ccggtacttc
    15721 accaccaaaa agaacggcaa aacgtactac atcaaactcc atgatccgcg cctgatgcgt
    15781 gcgatgaaga atatgggacc ggaaaccagc aatgcctttg ttcgtacgct ggggaaagtt
    15841 aaccgcttcc tggcaacggt gaacacgtcg tataacccgg aattcctggt cagtaacttc
    15901 atccgtgacg tgcagacggc ggtgatgaac ctgaaggcgg agcagggaag gagcgacggt
    15961 aaactgaaag ggctggataa cttatccgcc ctggctgtgg tgaaagacag ccgttctgcc
    16021 atgtcagccg tatacgccag tctgcgtggt aaaaccctca cgggaaaagg tgcacagtgg
    16081 cagaaggtgt ggaaagagtt tgttgaggac ggaggtaaaa ccggctggtt taacatgggt
    16141 gaccttgaag gccagcagaa ggaaatggat cgccttgtat cgctggcgaa gggaggatgg
    16201 aaaggccaga gtatcggtgc atggaattcg ttccttaacc ttgtcgagga tgccaacggg
    16261 gcggttgaaa acgctctgcg tctttctgcc tataaacacg cccgtgatgc cggtttgtca
    16321 cgccagcagg cggcgtctct tgccaaaaac atgacggtga actttaaccg tcgtggtgag
    16381 cagggagcgc tgatgaactc gctgtacatg ttcgccaacg ccagcattca ggggaccgca
    16441 aacctggtga gaacgctcgg acatcttaat ggcgacgggc cgttactgga gcgccttcgc
    16501 tggaagaatc tcaatgtacc gcagaaaatc gcgcttgcag ctgtgggagc gggttatctg
    16561 cttggctcgc ttaaccgcag tgttgcgggg gaggatgatg acggggttaa ctggtatgac
    16621 aaggtgccgt ctcatgtgaa agagcgtaac ctcgtcatta tgaaatcggt gttcgggggc
    16681 aaggccggag agtactggag tattcctctg ccttacgggt acaacgtttt cttcctgctt
    16741 ggtcacacag ccgaaggggt ggcagcgggc gacctgaccg cttcacgtgc tgccggtaat
    16801 gttgttggtg gtgtgcttgg ggcattcagt cctgtgggca gtgaaacgtc ggaaacactg
    16861 tccggggcat tgctgaaaaa tgcagcaccg acaattctgc gtccgtttgc gaatcttgcc
    16921 atgaacgaaa acttcatggg ggcgcagatt taccaggaga acatgccgtt tggtacacca
    16981 aaacctgaca gccagctggg aagacgttca acgccagaag cgtacaaggc gtttgcatcc
    17041 tggctgaatg cgttctctgg tggcagccag taccgttccg gtgcggtgga tgtcacgccg
    17101 gaatcactga aatactggat tgactatatc tccggaggga cagggcgctt catttccaaa
    17161 accacggatg cggcggtgaa atcgctgaac ggtattgata taccggagca gcaggtgccc
    17221 ttcctgggga aaatttcagg tgaggtgatg ccgtatgctg accagcagaa gatgtacgac
    17281 cggatgacag aggttgcgca gtatcacgca gagctgaaga gtctgaccgg tgcagaaaga
    17341 acggcgttca ttgacgagaa caacggaaaa ttgtcgatga acgggcttat gcaggatacc
    17401 cggaagagac tgaaggattt gcgtaaacag cgtgatgcca tttacgccga cagtactctc
    17461 agtctggcgc aacagtcggc gatggtgaaa tcggttgagc gggatatgaa aattgccgtg
    17521 gatcggttta accgcgagta caacaaaaaa gtgggagtgg agtaaagaac atgccccgta
    17581 cggaagtgcg gggcatgctt aacggggtgt gtacataaat gggtactgat atgacgatta
    17641 tttcgccact ttctgtattt aaatcaatga cttaatactt atattcatta tatccattta
    17701 actaagagga caatgcggca tgagtatacc cgctaatgga ctgcggggta agtacgctgc
    17761 cgctcgattg cttaaaccct cgccatttat gccgggtttt tatcattttt cttaatgttt
    17821 tccgcacgtt ctgctttttg gcgtgcttct gctttgcgct tgttgctcat gtcgttacgg
    17881 atctgtgcat gactcattaa cgcgaagata aaggtgccgc cgcagatgtt ccccgctaaa
    17941 gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca gcgtaccgtt aaacaccaga
    18001 tagaggattt caacagaacc gacaacgatg tgagtggtgt cacccagggc aataagccag
    18061 gtcatcaata taatcaccac aatctttgcc gcacccgctg caggaaacat ccataccata
    18121 gtggcgatca gccagccgga aatgatcgcg ttggcaaaca tctcgctggg ggtgttcttc
    18181 atcacatcca tgccgatttt gacaaatgca tcgcgagttt cttcattgaa gattggcata
    18241 tattcaaatg cccacgccgc aatacctgtc ccgagaatat tacccagcag cacgacgccc
    18301 cataaccgca taagtaagcc aacgttgctc attgtcggtt tttgcatgac gggtagtacc
    18361 gcagtcacgg tgttttcggt aaataattgc tggcgggcca taatgacgat aataaaacca
    18421 aaggtataac cgagattctc cagcaagaag ctgcccggca caccttccag ttcgacatga
    18481 aatatccctt ttgccagtaa tgaagcgccc atcgacagac ccgccgcaat ggctgaccac
    18541 agtagtgcca ttgcgtcgcg ttccagctct ttttcaccat cctggcggat atgctcatga
    18601 attgccatcg cccgggaggg gagtcgatct tcatctattt ctattttttt gccacgctct
    18661 ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat caattttgtc gttgtccatg
    18721 gtctcttctc ttgaattagc acgtatagct aaagcgtagc ggcttttttg ctcgcaactg
    18781 gcgggagtta ctttgaaaat gtagaaaagg ccgcgtttgc ctttttctgt ttctatagaa
    18841 tcaagtagcc tacagggcgg cgattaccag gctatgatca aatcagcaaa tcagggcgtc
    18901 tggacatcag ttgacgtgct gttacaatcg cccacaccta aacaggcgga tacggtatcg
    18961 ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac agggagacat ttatgaagct
    19021 tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg gggtgtgcga gttccggtac
    19081 agatcagcaa gggcgttctg acccgttaga agggttcaac cgcaccatgt acaactttaa
    19141 cttcaatgta ttagacccgt atattgttcg accggtcgct gtcgcctggc gtgattatgt
    19201 tccgcaaccg gcgcgtaacg gtttgagcaa ttttaccggc aaccttgaag aacctgcggt
    19261 gatggttaac tacttcttgc agggcgaccc ttatcagggg atggtccact ttacccgctt
    19321 tttcctgaac accattttgg ggatgggcgg ctttattgat gttgcaggga tggcgaaccc
    19381 gaaactgcaa cggactgaac ctcaccgctt cggtagtacg cttggtcatt atggcgtggg
    19441 ttatggcccg tacgttcaat taccgttcta cggtagcttc acgctgcgtg atgacggtgg
    19501 tgatatggcg gatggtcttt acccggttct ttcctggctg acctggccga tgtctgtggg
    19561 taaatggacg cttgaaggta tcgaaacccg cgcacagctg ctggattccg acggtctgct
    19621 gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg tacttccagc gtcatgattt
    19681 catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg aacgcgcaag cgattcagga
    19741 tgatttaaaa gatatcgatt ctgaataaga aacaaataaa aaaggtgagt cgcaaaactc
    19801 accttttttg ttgttatccc tcaattacca caagtaaaag gagataaacc gacaatgacc
    19861 cg
//
LOCUS       DABSYT020000081        19712 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.081, whole genome shotgun sequence.
ACCESSION   DABSYT020000081 DABSYT020000000
VERSION     DABSYT020000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.081"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            147..764
                     /locus_tag="ID725_RS17455"
                     /old_locus_tag="pgaptmp_003491"
     CDS             147..764
                     /locus_tag="ID725_RS17455"
                     /old_locus_tag="pgaptmp_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGV7852007.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(761..2443)
                     /gene="ligB"
                     /locus_tag="ID725_RS17460"
                     /old_locus_tag="pgaptmp_003492"
     CDS             complement(761..2443)
                     /gene="ligB"
                     /locus_tag="ID725_RS17460"
                     /old_locus_tag="pgaptmp_003492"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGV7852008.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            2701..3324
                     /gene="gmk"
                     /locus_tag="ID725_RS17465"
                     /old_locus_tag="pgaptmp_003493"
     CDS             2701..3324
                     /gene="gmk"
                     /locus_tag="ID725_RS17465"
                     /old_locus_tag="pgaptmp_003493"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGV7852009.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            3379..3654
                     /gene="rpoZ"
                     /locus_tag="ID725_RS17470"
                     /old_locus_tag="pgaptmp_003494"
     CDS             3379..3654
                     /gene="rpoZ"
                     /locus_tag="ID725_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGV7852010.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            3673..5781
                     /gene="spoT"
                     /locus_tag="ID725_RS17475"
                     /old_locus_tag="pgaptmp_003495"
     CDS             3673..5781
                     /gene="spoT"
                     /locus_tag="ID725_RS17475"
                     /old_locus_tag="pgaptmp_003495"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGV7852011.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            5788..6477
                     /gene="trmH"
                     /locus_tag="ID725_RS17480"
                     /old_locus_tag="pgaptmp_003496"
     CDS             5788..6477
                     /gene="trmH"
                     /locus_tag="ID725_RS17480"
                     /old_locus_tag="pgaptmp_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGV7852012.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            6483..8564
                     /gene="recG"
                     /locus_tag="ID725_RS17485"
                     /old_locus_tag="pgaptmp_003497"
     CDS             6483..8564
                     /gene="recG"
                     /locus_tag="ID725_RS17485"
                     /old_locus_tag="pgaptmp_003497"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGV7852013.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(8549..9415)
                     /locus_tag="ID725_RS17490"
                     /old_locus_tag="pgaptmp_003498"
     CDS             complement(8549..9415)
                     /locus_tag="ID725_RS17490"
                     /old_locus_tag="pgaptmp_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852014.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(9418..10623)
                     /gene="gltS"
                     /locus_tag="ID725_RS17495"
                     /old_locus_tag="pgaptmp_003499"
     CDS             complement(9418..10623)
                     /gene="gltS"
                     /locus_tag="ID725_RS17495"
                     /old_locus_tag="pgaptmp_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGV7852015.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10903..12294
                     /gene="xanP"
                     /locus_tag="ID725_RS17500"
                     /old_locus_tag="pgaptmp_003500"
     CDS             10903..12294
                     /gene="xanP"
                     /locus_tag="ID725_RS17500"
                     /old_locus_tag="pgaptmp_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGV7852016.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            12415..14124
                     /locus_tag="ID725_RS17505"
                     /old_locus_tag="pgaptmp_003501"
     CDS             12415..14124
                     /locus_tag="ID725_RS17505"
                     /old_locus_tag="pgaptmp_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGV7852017.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(14177..16495)
                     /gene="yicI"
                     /locus_tag="ID725_RS17510"
                     /old_locus_tag="pgaptmp_003502"
     CDS             complement(14177..16495)
                     /gene="yicI"
                     /locus_tag="ID725_RS17510"
                     /old_locus_tag="pgaptmp_003502"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGV7852018.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(16505..17887)
                     /locus_tag="ID725_RS17515"
                     /old_locus_tag="pgaptmp_003503"
     CDS             complement(16505..17887)
                     /locus_tag="ID725_RS17515"
                     /old_locus_tag="pgaptmp_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGV7852019.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            18180..18274
                     /locus_tag="ID725_RS17520"
                     /old_locus_tag="pgaptmp_003504"
     tRNA            18180..18274
                     /locus_tag="ID725_RS17520"
                     /old_locus_tag="pgaptmp_003504"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18214..18216,aa:Sec,seq:tca)
     gene            18470..19651
                     /locus_tag="ID725_RS17525"
                     /old_locus_tag="pgaptmp_003505"
     CDS             18470..19651
                     /locus_tag="ID725_RS17525"
                     /old_locus_tag="pgaptmp_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7852020.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
BASE COUNT         4883 a         5332 c         4983 g         4514 t
ORIGIN      
        1 agaagtcaga agaatgcatc tacgctacag aaaatagcga tttcacataa ctacaagtta
       61 tcaatttcct ctcaccttaa aaaatctcaa tcgtgacaat acgcacaaat cgctaccctg
      121 ccagacagat ttttagggag agaaccatgc tgttacacat tttgtatttg gttggcatta
      181 ctgccgaagc catgacgggg gctctggcgg ccgggcgacg ccgcatggat acatttggcg
      241 taattattat tgcgaccgcc accgcaattg gcggagggtc agtgcgcgat attctgctgg
      301 gccactatcc gctcggctgg gtcaaacacc cggaatatgt gattatcgtc gctaccgccg
      361 cagtactcac cactatcgtt gctcctgtaa tgccttacct gcgcaaagtg tttctggtgc
      421 tcgatgcgct tggactggtg gtcttttcta tcatcggcgc acaggttgca ctggatatgg
      481 ggcacggccc aattattgcc gttgtcgcgg cggtaaccac cggcgtgttt ggcggcgttt
      541 tgcgcgatat gttctgtaaa cgtatcccac tggtattcca gaaagagttg tatgccggtg
      601 tctcatttgc ctccgccgtg ctgtacatcg cgctgcaaca ctatgtttct aaccatgatg
      661 tggtaattat ctccaccctg gtattcggct tttttgcccg cttactggcg ctacgcctta
      721 agctgggatt accggtcttt tactacagcc acgaaggaca ctaaggttca aaacctgtga
      781 tctgctgggc agccagccaa ctgcccagct tcttgatttg cgcattttcc ttccattcaa
      841 taacctgtct ggcgcgcccc gatccagtcc ccggcagctg ctgccagaac tgctccgtgc
      901 taaataaaag ttgcgaccag gaccgttcat cactggcatt aagcgccgcc cgggttagcg
      961 gtattcccat tgccatcacc cagcgagtaa aaggctgctt acgagccaga ttaaaccgat
     1021 gccatagctg cgcactttta cttttcgcga tccccggcgt gttctgtaat tgctctggcg
     1081 ttaataaaag ccaggaaaag atatgctcaa agcgatgagt ctgatgcagc gcgcgccagc
     1141 cggcctcacc aatgccatcc agccccagaa cctgttttga ccccagccag actaagcgtg
     1201 aaatgaactg ttcctgacaa acatcagaag caaagtagca ggtcaacgag ttaaagcggt
     1261 tttctggtgg tgtcggtttt gtacgttctg caccgcgcca gaccacatca tcaatgcgag
     1321 gaataccctg accggcaagg ctgacgagga tctgatcacc aggcgcaata tcccactcct
     1381 gccagcgcct gacggaacca atattcaccc gctggacttt tttatcatcc agcatgacag
     1441 gtgcgagtga cgcaaccacc gatattttac cgctcttacc caccgcaaac tgaatcgcct
     1501 tcactttggc aacctgagct acaggttgat atttccaggc caccagccat tctgcctggc
     1561 ctggtagcca atggcgggat tcgggttctt tcgcccctcg cacaaccacg ccatcggtga
     1621 cgaagggtaa tttcgctttc caccactcat tgcgtacgcg cgcaacctca tcagcatttt
     1681 taaccgcacg ggtatacctc tgcgttagag taaaacctgc ggtagccagc tcctttaaac
     1741 gatcggtcat taactgcggt ccatccggcc atgcccagac aaaaacgccc agagaattca
     1801 gcgtgtcgct atcgtcctgg cgcatcatca ggccagcaac ttttgcgcgg gcatttattc
     1861 cccccatttg ttgttggata tgcccctcgc gctggagaaa tatttccccc tgaagcgtac
     1921 tgttggctaa aggcccgcta acggtttgcg gcacagcgga aattaagcta actttctgcg
     1981 tccagtcctc gcctttcagg ccattaccgc gactgattgc tttgttcagt ttcccgtccc
     2041 gataaaccag ggttaccgca acgccatcaa cttttggctg cacccaaaga tcgctacgtt
     2101 ctcgcatcca cagacttaat gcgtttttat ccgccatttt gcgtacaccc gtatgggcaa
     2161 ccggatgcat aaccgcacca tttaaaggcg gcatcatcac gtcgcggggc tcgctcacaa
     2221 agcagcgttg ccactgcgta agacgggcgc ttaactgatc gtaaacaccg tcttccacct
     2281 cactttttcc ttccttccag tagtcatcgt cccactgttt tatttgctgt tgcaggcgga
     2341 aaatttcttc ctgtgctctg gctggcgacc aggccggaca gaccgcccac acagatgatt
     2401 gccagcacaa gatacttatt aatatcgcca tccatacttt catcttcacc tccgctgtag
     2461 tcaggctggc agatatacaa cgtggtgaag gcaaagccga gtggcaaaaa cggagtctgc
     2521 gaggacgctt cctgagaatc gtctttattg cagtaaatca caggcaaatg cggaaacagc
     2581 tacgcaaaac gcaacaactt tgcgcaaaaa gtgtgagcaa gggctacgtc acatggccgc
     2641 gccgtgtata ataagctcgt atgtaggctt tatttcgcta atcacatacg aaagatactc
     2701 atggctcaag gcacgcttta tattgtttct gcccccagtg gcgcgggtaa atccagcctg
     2761 attcaggctt tattaaaaac ccaaccgttg tatgacaccc aggtttctgt ttcacacacc
     2821 acacgccaac cgcgtcctgg tgaagtccac ggtgaacatt atttctttgt taatcatgat
     2881 gaatttaaag aaatgattag cagagatgcg ttcctcgaac acgcagaagt ttttggtaat
     2941 tactatggca cttcgcgtga ggccattgag caagtactgg cgaccggtgt cgatgttttt
     3001 ctcgatatcg actggcaggg cgcgcagcaa attcgccaga agatgccgca cgcgcggagt
     3061 atctttattt taccgccgtc taaaattgaa ctggaccgcc gtctacgcgg tcgcgatcag
     3121 gacagcgaag aggtcattgc aaagcgtatg gcgcaagctg ttgcagaaat gagccattac
     3181 gccgaatatg attacctgat tgtgaatgat gacttcgata ccgcgttgac cgatttgaag
     3241 accattattc gcgccgaacg tctgcgcatg agccgccaaa agcagcgtca tgacgcttta
     3301 atcagcaaat tgttggcaga ctgaacctga tttcagtatc atgcccagtc atttcttcac
     3361 ctgtggagct ttttaagtat ggcacgcgta actgttcagg acgctgtaga gaaaattggt
     3421 aaccgttttg acctggtact ggtcgccgcg cgtcgcgctc gtcagatgca ggtaggcgga
     3481 aaggatccgc tggtaccgga agaaaacgat aaaaccactg taatcgcgct gcgcgaaatc
     3541 gaagaaggtc tgatcaacaa ccagatcctc gacgttcgcg aacgccagga acagcaagag
     3601 caggaagccg ctgaattaca agccgttacc gctattgctg aaggtcgtcg ttaatcacaa
     3661 agcgggtcgc ccttgtatct gtttgaaagc ctgaatcaac tgattcaaac ctacctgccg
     3721 gaagaccaaa tcaagcgtct gcggcaggcg tatctcgttg cacgtgatgc tcacgagggg
     3781 caaacacgtt caagcggtga accctatatc acgcacccgg tagcggttgc ctgcattctg
     3841 gccgagatga aactcgacta tgaaacgctg atggcggcgc tgctgcatga cgtgattgaa
     3901 gatactcccg ccacctacca ggatatggaa cagctttttg gtaaaagcgt cgccgagctg
     3961 gtagaggggg tgtcgaaact tgataaactc aagttccgcg ataagaaaga ggcgcaggcc
     4021 gaaaactttc gcaagatgat tatggcgatg gtgcaggata tccgcgtcat cctcatcaaa
     4081 cttgccgacc gtacccacaa catgcgcacg ctgggctcac ttcgcccgga caaacgtcgc
     4141 cgcatcgccc gtgaaactct cgaaatttac agcccgctgg cgcaccgttt aggtatccac
     4201 cacattaaaa ccgaactcga agagctgggt tttgaggcgc tgtatcccaa ccgttatcgc
     4261 gtaatcaaag aagtggtgaa agccgcgcgc ggcaaccgta aagagatgat ccagaagatt
     4321 ctttctgaaa tcgaagggcg tttgcaggaa gcgggaatac cgtgccgcgt cagtggtcgc
     4381 gaaaagcatc tttattcgat ttactgtaaa atggtgctca aagagcagcg ttttcactca
     4441 atcatggaca tctacgcttt ccgcgtgatc gtcaatgatt ctgacacctg ttatcgcgtg
     4501 ctgggccaga tgcacagcct gtacaagccg cgtccgggcc gcgtgaaaga ctatatcgcc
     4561 attccaaaag cgaacggcta tcagtcgttg cacacctcga tgattggccc gcacggcgtg
     4621 ccggttgagg tccagatccg taccgaagat atggatcaga tggcggagat gggtgttgcc
     4681 gcgcactggg cttataaaga gcacggcgaa accagtacta ccgcacaaat ccgcgcccag
     4741 cgctggatgc aaagcctgct ggagctgcaa cagagcgccg gtagttcgtt tgaatttatc
     4801 gagagcgtta aatccgatct cttcccggat gagatttacg ttttcacacc ggaagggcgc
     4861 attgtcgagc tgcctgccgg tgcaacgccc gtcgacttcg cttatgcagt gcataccgat
     4921 atcggccatg cctgcgtggg cgcacgcgtc gaccgccagc cttacccgct gtcgcagccg
     4981 cttactagcg gtcagaccgt tgaaatcatt accgctccgg gcgctcgccc gaatgccgct
     5041 tggctgaact ttgtcgttag ctcgaaagcg cgcgccaaaa ttcgtcagtt gctgaaaaac
     5101 ctcaagcgtg atgattctgt aagcctgggc cgtcgtctgc tcaaccatgc tttgggtggt
     5161 agccgtaagc tcaatgaaat cccgcaggaa aatattcagc gcgagctgga tcgcatgaag
     5221 ctggcaacgc ttgacgatct gctggcagaa atcggccttg gtaacgcaat gagcgtggtg
     5281 gtcgcgaaaa atctacaaca tggggacgcc tccattccac cggcgactca aagccacggg
     5341 catctgccga tcaaaggcgc tgatggcgtg ctgatcacct ttgcgaaatg ctgccgcccc
     5401 attcctggcg acccgattat cgcccacgtc agccccggta aaggtctggt gatccaccat
     5461 gaatcctgcc gtaacatccg tggctaccag aaagagccag agaagtttat ggctgtggaa
     5521 tgggataaag agacggcgca ggagttcatc accgaaatca aggtggagat gttcaatcat
     5581 cagggtgcgc tggcaaacct gacggcggca attaacacca cgacctcgaa tattcaaagt
     5641 ttgaatacgg aagagaaaga tggtcgcgtc tacagcgcct ttattcgtct gaccgctcgt
     5701 gaccgtgtgc atctggcgaa tatcatgcgc aaaatccgcg tgatgccaga cgtgattaaa
     5761 gtcacccgaa accgaaatta atgttttatg aacccaacac gttatgcacg catctgcgaa
     5821 atgctcgcca ggcggcagcc tgatctgacc gtctgcatgg agcaggtcca caaacctcat
     5881 aacgtttctg cgattattcg taccgcagat gccgttggcg tacatgaagt tcacgccgtc
     5941 tggcctggta gccgcatgcg caccatggct tcggcagcgg cgggtagtaa cagctgggta
     6001 caggtgaaaa cacaccgcac cattggcgat gccgtcgctc atctcaaagg ccagggcatg
     6061 cagattctgg caacccatct ttctgataac gctgtcgatt tccgcgaaat tgattacact
     6121 cgcccgacct gcattttgat gggacaggag aaaacgggca tcacgcagga agcattggcc
     6181 ctggcggatc aggacatcat cattccgatg atcggcatgg tgcagtcgct gaatgtttcc
     6241 gttgcctcag ccctcattct ttacgaagcc cagcgtcagc ggcaaaatgc aggcatgtac
     6301 ctgcgtgaaa acagcatgtt gccggaagca gagcaacaac gcctgttgtt tgaaggcggc
     6361 tatccggtgc tggcgaaagt cgcaaaacgc aaaggcctgc cttatcccca cgtcaatcag
     6421 caaggcgaga tcgaagctga tgccgactgg tgggctacta tgcaggctgc agggtaagtg
     6481 ccatgacagg tcgcctgtta gatgctgtcc cactcagttc cctaacgggc gttggcgcag
     6541 cacttagtaa caaactggcg aaaatcaacc tgcataccgt gcaggatcta ctcttacacc
     6601 ttcccctgcg ctacgaagat cgcacccatc tctaccccat cggagaacta ctgccgggcg
     6661 tttatgccac ggtggaaggc gaagtgctga actgcaatat ctccttcggc ggtcggcgga
     6721 tgatgacctg ccagatcagc gacggttccg gcatcctcac catgcgcttt ttcaatttca
     6781 gcgcggcaat gaaaaatagc ctggcgacgg gccgccgtgt actggcttat ggcgaagcaa
     6841 agcgcggtaa atatggtgcg gagatgatcc acccggaata ccgcgtgcag ggcgatctca
     6901 gcacgccaga attacaggaa acgctcacgc cggtttatcc aacaacggaa ggcgtaaagc
     6961 aggccacgct gcgtaaatta accgaccagg cgctggatct gctcgacacc tgcgccattg
     7021 aagaactcct gccgccggaa ctgtcacaag gaatgatgac gctaccggaa gcgttgcgca
     7081 ctttgcaccg cccgccaccg acgctacagc ttagcgatct ggaaaccggg cagcatccag
     7141 cgcaacgtcg tctgattctg gaagaactgc tggcgcacaa cctcagcatg ttagccttac
     7201 gtgccggagc acagcgtttt catgcccagc cgctgagcgc caatgatgcg ctgaaaaata
     7261 aactcctcgc agccttaccg ttcaagccaa cgggcgcaca ggcacgcgta gtggcggaga
     7321 tcgagcgcga tatggcgctg gatgtgccga tgatgcgcct ggtacagggc gatgtaggtt
     7381 ccggtaaaac gctggtcgcc gccctcgccg cgctgcgtgc gattgcccac ggcaaacagg
     7441 tggcattgat ggcaccaacc gaattactcg ccgaacagca cgccaataac ttccgcaact
     7501 ggtttgaacc gctcggtatc gaagtgggct ggctcgccgg taagcagaaa ggcaaagcgc
     7561 gactgtctca gcaggaagcc atcgccagcg gtcaggtgca gatgattgtt ggtacgcacg
     7621 ccatcttcca ggaacaggtg cagtttaacg gtctggcgct ggtgattatc gacgaacaac
     7681 atcgctttgg cgtgcatcag cgtctggcgt tatgggagaa aggccagcag caaggcttcc
     7741 atccgcatca gttgatcatg accgccacgc cgatcccccg cacgctggca atgacggcgt
     7801 atgccgatct cgatacctcg gtgatagatg agctgccgcc aggccgcacg ccagtgacta
     7861 cggtcgctat tcctgatacc cgccgtaccg acatcattga ccgcgtgcgc cacgcctgca
     7921 taactgaagg tcgtcaggcg tactgggttt gtacgttgat tgaagagtca gagttactgg
     7981 aagcgcaggc ggcggaagct acctgggaag agttgaaact ggcgctacca gaattgaatg
     8041 ttggcctggt acacgggcgg atgaaaccag ccgagaaaca ggcggtgatg gcgtcgttta
     8101 aacaaggtga gctacatctg ctagttgcca ctaccgttat tgaagtcggt gttgatgtgc
     8161 ctaacgccag cctgatgatt atcgaaaacc ctgagcgtct gggtctggcg cagttacacc
     8221 agcttcgcgg gcgtgtaggt cgtggtgcgg tggcttctca ctgcgtgctg ctctacaaaa
     8281 cgccgctctc caaaaccgcg caaattcggc tgcaagtgct gcgcgacagc aacgacggtt
     8341 ttgtgattgc acagaaagac ttagagattc gcggaccggg tgaactgtta ggtacgcgtc
     8401 agacgggtaa tgctgagttt aaagtggcgg atttactgcg cgatcaggcg atgatcccgg
     8461 aagttcagcg cctggcgcga catattcacg aacgttaccc acaacaagca aaagccctga
     8521 tagaacgctg gatgccggag acggaacgtt actccaacgc atgaccagac tcatcacaag
     8581 acaagtcacg cagtaaatct tcaatctgga tagtgaggat atccattgcc tcatacgcat
     8641 cctgtgcaat tcgcgccgcc attgcgcctt ccggttcacc acgcagaatt tcagccgctg
     8701 cctgtacgcc agccacaatc atttcaggtg ttaacatttg gcgaagaaaa gcgcgattat
     8761 cagaggacca gccatcttta tggcctaatg gatgccccca gcctatccat tcttgcgacc
     8821 ataacggatt ggcttctatg ggataaatta gatgcgcggc atactgaata gcacacaccg
     8881 cttttcctgg cgtatccagc gcccaccagc gcgaccagag agaacgaatt aacggcgcgg
     8941 ccccacccag agtattgaac accgccagcc agcaaagaac gttattgaaa ccacgctcgt
     9001 tatccatacg ctgcaacgcg gtgtccagta aaaattgccg tacctgctgg cgttgggaag
     9061 cattcatcat ctcctgccag agatagggcc gacacaatgc cttcattaag gattcttcga
     9121 agtgatcacc ataaccgcca gctaaaacgg tttcatgcca ttgcgccagg cagagcggaa
     9181 acagatagcg aaacaactct atttgcatcg gttcgttata aacgagattg agtacatagt
     9241 aagcactcaa atcccattcg ctgatctgct gccactcttt agccgccagc gccttgagtt
     9301 cagcatctgc gtaatcaaat tccgtttccc atacacagag cactggcggc tcatcaccaa
     9361 aaatatggcg acagtgttca cgtagcgttt ccggttcctt ccgacgcttt ttcattttta
     9421 accggcaaaa atcggcaaca ttaaatacag cttaatcacc agcgcattga cgatatcgat
     9481 aaagaacgca ccgaccatcg gcaccaccaa aaacgccatg tgcgacgggc caaagcgttc
     9541 agtgatcgcc tgcatgttgg cgattgccgt tggcgttgca ccgaggccaa aaccacagtg
     9601 acccgcagcc agcactgccg catcgtagtt tttgcccatc atgcgccagg taacgaagat
     9661 ggcatacaac gccatgaaga tggtctgtac caccagaatc gccagcatcg gcagcgccag
     9721 cgaagccagc tcccacagtt tcagccccat cagcgccatc gccaggaaca acgacaggct
     9781 tacgttaccc agcacggata ccgcacgctc aaagacgcgg taaaagccca tcattgacag
     9841 accgttgctc agaatcacgc caacaaacag tacacagacg aaggtcggca gttcaaaagc
     9901 agtgccagcc aaaagttgcg caacaatttt ccccaccgtc aggcagatag caatcagcgc
     9961 gatagtttca atcagcacca gcgaggtgat catgcggccc acatccggct tttcaaacgc
    10021 cgtcgggact tcctggtcat ccggaatacc gttcggcgtg gtggagtgtt tcaccagata
    10081 gcgcgccacc ggaccgccaa tcaagccgcc cagcaccaga ccgaacgttg cacaggccat
    10141 cgccacttcc gtcgcattgg tgaagccata acgttcaatg aacaatttac tccacgcagc
    10201 gcccgtaccg tgaccgccgg aaagcgtaat agaaccggcc aacagcccca tcagcggatc
    10261 aagccctaac aggctagcca taccaatgcc aatggcattt tgcatcacca acagaccaac
    10321 aaccacaatc aagaagatgc caaccacacg cccaccggca cgcaaactgg caatgttggc
    10381 gttcaggcca atggtggcga agaaagccag cattaacgga tcgcgcaggg acatatcaaa
    10441 gttgacttcc cagcccatgc tttttttcag tactagtagc gccagcgcca ccaacaaacc
    10501 acccgcaaca ggttccggta tggtgtattt cttcaaaaag gagacggaat ggaccaactt
    10561 acgcccgagc aacaacgtca gcgttgcggc aacaagcgtt gctaaagtat cgagatgaaa
    10621 catagttact cctttgtatc cgcatcactc tttcatactc gtcatacttc atcgcgcagt
    10681 ggcaacttca tgaactatga cgggtatcga gttatgcttt cctgggtcaa attctcagct
    10741 gttaatgaac aaatggcatg gattttaagc agaaaaatac caaaagttat ataaaaaagt
    10801 gcatttatct catttaaata tttagatgag gatgtggcaa acgtttgctt ttctctttcg
    10861 gtcagctaaa atgcccgctt tgctaccacg ggattgtttt cgatgtctgt ttccaccctc
    10921 gagtcagaaa atgcgcaacc ggttgcgcag actcaaaaca gcgaactgat ttaccgtctt
    10981 gaagatcgtc cgccgcttcc tcaaaccctg tttgccgcct gtcagcatct gctggcgatg
    11041 ttcgttgcgg tgatcacgcc agcgctatta atctgccagg cgctgggttt accggcacaa
    11101 gacacgcaac acattattag tatgtcgctg tttgcctccg gtgtggcatc gattattcaa
    11161 attaaggcct ggggtccggt tggctccggg ctgttgtcta ttcagggcac cagcttcaac
    11221 tttgttgccc cgctgattat gggcggtacc gcgctgaaaa ccggtggtgc tgatgttcct
    11281 accatgatgg cggctttgtt cggcacgttg atgctggcaa gttgcaccga gatggtgatc
    11341 tcccgcgttc tgcatctggc gcgccgcatt attacgccgc tggtttctgg cgttgtggtg
    11401 atgattatcg gcctgtcgct aattcaggtt gggttaacgt ccattggcgg cggttacgca
    11461 gccatgagcg ataacacctt cggcgcaccg aaaaatctgc tgctggcagg cgtggtctta
    11521 gccttaatta tcctgcttaa ccgtcaacgt aacccttact tacgcgtggc ctcactggtg
    11581 attgcgatgg cggccggata tgcgctggcg tggtttatgg gcatgttgcc agaaagcaac
    11641 gaaccgatga cgcaagaact gattatggtg ccaacgccgc tctattacgg tcttggcatt
    11701 gaatggagtc tgctgctgcc gctgatgctg gtctttatga tcacttcgct ggaaaccatt
    11761 ggcgatatca cggcgacctc tgacgtttcc gaacagccag tgtccggtcc gctgtacatg
    11821 aaacgcctga aaggcggcgt gctggcaaac ggcctgaact cgtttgtttc ggcggtgttt
    11881 aacaccttcc cgaactcctg cttcgggcaa aacaacggag tgatccagtt gactggtgtt
    11941 gccagccgct atgtcggttt tgtcgtcgcg ctgatgttga tcgtgctggg tctgttcccg
    12001 gcagtgagcg gttttgtaca acacattcca gaaccggttc tgggcggcgc aacgcttgta
    12061 atgtttggca ccatcgccgc ctccggtgtg cgtatcgttt ctcgtgagcc gctgaaccgt
    12121 cgggcgattc tgattatcgc gctgtcgctg gcggttggtc tgggcgtgtc tcagcagccg
    12181 ctgattttgc agtttgcccc tgaatggctg aaaaacctgc tctcctccgg gatcgccgcg
    12241 ggcggtatta ctgccatcgt gctgaatctg attttcccac cagaaaaaca gtaattcact
    12301 ggcggcggta agataattac cgccgcagtc tcacctcttt caccattccc gccttgagga
    12361 ttgcgcgtaa atcgtgcata actcccttat gtgcatttca cgggatggaa gaccatgaaa
    12421 tttattggga agctgcttct ctacattctc atcgctctgt tagtggtgat cgctggcctc
    12481 tattttcttc tgcaaacccg ctggggagca gaacatatca gcgcatgggt ttccgagaat
    12541 agcgactatc atctggcctt cggggcgatg gatcaccgtt tttccgcgcc atctcatatc
    12601 gtgctggaga acgtcacgtt tggtcgtgat ggtcagcccg cgaccctggt ggcaaaaagt
    12661 gtcgacattg cgctaagcag tcggcaactg accgaaccac gccatgtcga taccatcctg
    12721 ctggaaaacg ggacgctgaa tctcaccgac cagaccgcgc cgctaccgtt caaagccgat
    12781 cgtctgcaac tgcgtgatat ggcgtttaat agcccgaata gcgaatggaa actgagcgcg
    12841 cagcgggtaa atggcggcgt ggttccgtgg tcaccagaag ccggtaaagt gctgggtacg
    12901 aaggcgcaga ttcagtttag tgccggatcg ctttcgctca atgatgttcc tgccaccaat
    12961 gtactgattg aaggcagtat tgataacgat cgcgttacgc tgactaacct gggtgccgac
    13021 atcgcccgcg ggacattaac cggaaacgcg cagcgtaacg ccgacggcag ctggcaagtg
    13081 gaaaatctgc gcatggcgga tatccgtcta caaagcgaaa aatcgctaac cgacttcttt
    13141 gcgccattac gctctgtccc gtcgttgcag attggtcgcc tggaagtgat cgatgctcgt
    13201 ttgcaaggtc cggactgggc ggtgaccgac ctcgatctca gcttgcgcaa catgaccttc
    13261 agtaaagatg actggcagac acaagaaggc aaactgtcga tgaacgctag cgagttcatt
    13321 tatggttcgc tgcatttatt tgacccgatt ataaacgcgg aattttcccc gcagggcgta
    13381 gcgctgcgcc agttcaccag ccgctgggaa gggggtatgg tcagaacgtc agggaactgg
    13441 ctgcgtgacg ggaaaacgtt gatccttgat gatgcggcaa ttgccgggct ggaatatacc
    13501 ttgccgaaaa actggcaaca gttgtggatg gaaacgacac ccggttggtt aaacagcctg
    13561 caactgaaga gatttagcgc cagccgcaat ctgatcattg atatcgaccc tgacttcccg
    13621 tggcagctca ccacgctcga tggttacggt gccaacctga cgctggttac cgatcataaa
    13681 tggggcgtct ggagtggctc ggcgaatctg aatgccgccg ccgcgacatt caatcgtgtt
    13741 gatgttcgtc gcccgtcgct ggcgctgacc gccaacagca gcacggtgaa tatcagcgaa
    13801 ctgagtgcat ttactgaaaa aggcattctg gaagccactg ccagtgtttc acaaacgcca
    13861 caacgtcaga cccatatcag cctgaatgga cgcggtgtgc cggtgaatat tttgcaacaa
    13921 tggggatggc ctgaattacc gttgactggc gacggcaata ttcagcttac cgccagtggc
    13981 gatattcagg ccaatgtccc gctgaaacct acggttagcg ggcaactcca tgccgtgaac
    14041 gccgcaaagc agcaagtgac tcaaaccatg aatgcgggcg tcgtttccag tagcgaagtt
    14101 acatcgacag agccggtgca gtaaaaatca atctcgccgg gtacgactca gacgtacccg
    14161 gcattccatc aatagattac aacgtaattg tcagcgcatt cccttgaggc gtcaccacca
    14221 gcccctgctc actttcagcc tgcgaaccac cttgcagacc atttactttc acaatattgc
    14281 gcaagcacag tgtccagttc ttcgcctcgc ccgcaccagt cacagtaatc gtgttgccag
    14341 tacgcgccgc tttcagggtg aaaagaacgg aaccgtcagc agcgggcact tcacagatgg
    14401 cttcatgccc gtcttgtaga ttaaagagct ggaatgccgt gccttcgtgc cacgcgtaat
    14461 cgggacgttg ctcgttgttg cccagcgcca gtagggtgtt atcacgcaca taaacgggca
    14521 gactcaggaa gctgtgctgc tgtttatgcc agcgactacc atcgagttca tcgttgtgcc
    14581 acagatgtgt ccagcgacct tccggcaagt agaactgcac atcgcccgct tcactgaaca
    14641 ccggagcaac catcacgttg tcgcctaaca tgtattgacg gtcaaggtaa tcacaagccg
    14701 gatcgtccgg gaactccatc atcatggccc gcatcatcgg cgtaccccgc gcgttcgcac
    14761 gagcagcttc acgatacaga tacggcatca tgcggcattt cagttgcgtg aagaagcgca
    14821 ccacatcaca ggactcatca tcgtaggccc acggcacacg ataagatttg ctgccatgta
    14881 aacggctatg gctggagagc aaaccaaacg cgcaccagcg tttgtaaacg tgcgccagag
    14941 cggtattctc aaagccgccg atatcgtggc tccagaaacc aaaacctgaa aggccaatag
    15001 acaaaccacc gcgcaggctt tccgccattg attcgtagtt agcgtaacag tcaccacccc
    15061 agtgtaccgg gaatttctgc gcaccaacgg aggccgagcg agcaaacaag acggcttctt
    15121 cctcgccaac ggtgtccttg agcacgttcc acaccagttc gttgtagatg tacgcataat
    15181 ggttatgcat tttctgcgga tcggaaccgt caaaccactg aacatcgatt ggaatacgtt
    15241 cgccaaagtc ggtcttaaag caatcaacgc ccatcgcgac cagacctttc agtttgtcgg
    15301 cgtaccattt gcaggcatcc ggattggtaa agtcataaat cgccagacct ggctgccatt
    15361 tatcccactg ccataacgaa ccgtccgggc gtttgagtaa atagcctttc tcttgtaact
    15421 ctttaaagac aggggatttt tgcccgatat aggggttaat ccagacgcag atttttagcc
    15481 ctttcgcttt caggcggcgg atcatccctt ccgggtccgg gaaagtcagc gggtcccact
    15541 caaaatcgca ccactggaag gctttcatcc agaaacagtc aaagtgaaaa acatgcagtg
    15601 gcagattgcg tcccgccata ccatcgataa agctgtttac cgtcgcttcg tcgtagttgg
    15661 tggtaaatga agtggttagc cacaggccga aggaccacgc gggcggcagc gccggacgac
    15721 cagtaaaacg ggtataacga tcaagtaccg ctttcggcgt cgggccgtcg ataacaaagt
    15781 attcgagata ttcactctca acgctgaact gcactttgga gactttctcc gatcccactt
    15841 caaaagagac gcattgagga tgattgacca gtaccccata gccacggtta gtcatataga
    15901 acgggatatt tttatacgcc tgttcagtac ttgtgccgcc gtcccggttc caggtctcta
    15961 ccgtctggcc attgcgcacc agggcagtaa agcgctctcc cagaccgtaa actgtttcgc
    16021 caacgccaag atccagccgc tcaaacatat aatttcgttg attattcgtg tcctgcacgt
    16081 agccattatt tttcacctga ctaccggtaa tacgttcgcc gttgcgcaga aaatccagtg
    16141 accagaactc acctttgctg acacgcgcgc ttaagttgcc acttttaaac tcagcgtaac
    16201 gttctgtgtt ttcgattgtg accttcacgt cctgcaaaat attgagcgga taatgaggac
    16261 cgttattcag cgccccctga aaatgctcaa tccgtacacc gacaatacct tcctgtgggg
    16321 agaaaaagcg cagcgtaaat aaaggcgtat caagctgcca ggttcgttca cgcacatcac
    16381 ggggggcagc atagaccacc atttcattac cctgctgttc aacctcgaac acctgaagcg
    16441 ggtgaatcaa attgaggcca ggttgaatca accagtttcc gtcgctaatt ttcattgccg
    16501 ttccttagtt ctgcacttct tgagaggtga attgttgctg gcaacggcgt ttgccctggg
    16561 ccagctgttc cataacggtt ttcagattgt gcgtcgtgag tgagtagtag cgtttagcga
    16621 taatcgcgct cagcaaataa cagatcgccg gaacaatcgt gaatagcgca ataatgatgc
    16681 taatcgtggc gctgttctgc gctttttctg ccgcatcata tccgccataa gccagcatcc
    16741 agccgataag agcgccgccg aaggccaacc ccagtttgag cacaaacagc gtgccagcaa
    16801 aactgatccc ggtcaggcgt ttaccattgc accactcgcc gtagtcgacg gtatcggaca
    16861 tcattaccca ctggataggt gtcaccagtt gatgcaatac accaatcacg aagatgaaga
    16921 caaacatagt gatgctggcc tgcataggaa caaagaacat cgcgaggcta atcactgcca
    16981 gcagggcgtt cgtccaccag aagatagtga ctttacattt ccagtcggtc agcggttttg
    17041 ccagtgcgga accaatcagg ttacccacgc aataagtggt gagaaaagcg acaaacactt
    17101 ccggcgtacc caaaatccat gtgacgtaat acatcatcgc tccaccgcgg acgcacaccg
    17161 ccaggatatt gaaaatggtt agtaaaccga caatccgcca ctggtcgttt tgccagatat
    17221 cacgtaaatc ttcccgcata gacgtcgttg taggtggtgc ttcaacgcgt tctttagtgg
    17281 tgaagaaaca gaatgccagc atcatgaatg ccaccacgga aaggaccgcg ataccgccct
    17341 ggaaaccgag tggtttatta tcaccgccaa ttaaattaac cagtggcatc atcagaacag
    17401 tagagagcat gcctcccgcg gtcgccagca caaaacgcca ggattgcagc gagatacgct
    17461 gagtcgggtc attggtgatt acaccaccca atgcgcagta agggatattg acgacggtat
    17521 aaagtaaggt aagtagggtg taagtaattg ctgcatagat cattttgccg ttcatactga
    17581 gatctggcgt gctataggcc agtacacaga cgatcccaaa tggcagtgcg ccacacagta
    17641 cccacggacg aaatttaccc cagcgagagc gcgttcgatc ggccaacaac cccatgcaag
    17701 gatcggaaat cgcatccagt gcacgagcga ccaaaaacat ggttccgaca aatccggcag
    17761 gtatgccaaa attatcggta taaaagaaca tcatatataa cattacgtta tcgaaaataa
    17821 tgtggctggc ggcgtctccc atgccataac caattttctc tttaacggac aacacttcac
    17881 tcttcatctg cttttccttg atgctatagg ggtgctgaga ccgataacgc attttgtaaa
    17941 ccatccctgt gagagcgaat attccgatat ctggttatta atttatgatt cttgttttat
    18001 gtgatcgtgg tagcgttaat tccgctcata tatcattgta aaatatgagt tttatatgaa
    18061 ttataatgct ttcgtgataa tacgctgcgt gtattaggcg gaaaaaactg atctggggga
    18121 tgtagaaact caaggaagta gctataatgc gccccgcctc catgtagcaa tcgaggcgcg
    18181 gaagatcgtc gtctccggtg aggcggctgg acttcaaatc cagttggggc cgccagcggt
    18241 cccgggcagg ttcgactcct gtgatcttcc gccagtttgc ctctcctgaa ctctcccgac
    18301 atcaacaaag ccccttataa tcccttatat atcaggtttt ctttgtatcc tgaggtcaaa
    18361 cgactttaac ccatatcaag ttgtttgtgg gggtgcattg ggggtatcag gttggttact
    18421 ggcggaaatt cccccaggag gatggattca tcaaaagctg gaggatgtca tggcgctaac
    18481 cgatgttaaa gtgaaaaccg cgaagccaaa agaaaggccc tataagctgg ctgatggcgg
    18541 aggtatgtat ctgctgatta acgctaatgg ttccaaatac tggcgtatga agtaccgctt
    18601 cgccggtaaa gaaaagatgc tgtctattgg cgtatacccc gatgtaacgc ttgccgacgc
    18661 acgtgagaaa cgcagtgaag ccaggaaaat acttgcggct ggtggcgatc cgggtgaggc
    18721 aaaaaaagaa gaaaaaatag cgctgcaaat gagtttgaaa aacactttcg aggccgttgc
    18781 tcgcgagtgg catcagacga aagccgatcg ctggtcactg cgctaccgcg atgaaataat
    18841 cgacaccttt gagaaagata tcttccccta catcggcaag cgacctattg ccgaaatcaa
    18901 gccgatggag ctattggaag cactacgtaa gatggagaaa cgtggagcac tggagaaaat
    18961 gcgcaaggtc cgccaacgtt gtggtgaggt gtttcgctat gcaattgtta ctggtcgagc
    19021 cgactataac cctgctcccg atcttgccag tgctttagct acaccgaaaa aagtacattt
    19081 tccctttctt actgccaatg aacttcccca cttccttact gatctggcgg gttataccgg
    19141 aagtatcatc actaagacag ctactcagat cattatgtta accggtgtac gaacgcaaga
    19201 gttgcgtttc gcgcattggg aggatattga ttttgaggca aaattatggg agatcccggc
    19261 agaagtaatg aagatgaaac ggcctcatat tgtgccgccc tctgagcagg ttattgcgct
    19321 atttaagcaa cttgaaccaa tctcaaaaca tcatcctctg gtctttatcg gtaggaacga
    19381 tcctcgcaag ccaattagta aggagagcat taaccaggtc attgaattgc tggggtataa
    19441 gggaagactc acagggcacg gtttcaggca taccatgagc acaattctgc acgaacaagg
    19501 ctttaattct gcctggattg aaatgcagtt ggctcatgtg gataaaaact ccatcagggg
    19561 tacctataac cacgcccagt atctcgatgg tcgccgtgaa atgatgcaat ggtacgctga
    19621 ctacattgat tcactttctg agctggcctg atgaatgaaa gttcggtgac acaaaaataa
    19681 tgaataacca tccagcccca acaggcggat gg
//
LOCUS       DABSYT020000082        19549 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.082, whole genome shotgun sequence.
ACCESSION   DABSYT020000082 DABSYT020000000
VERSION     DABSYT020000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.082"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..403
                     /gene="nudI"
                     /locus_tag="ID725_RS17530"
                     /old_locus_tag="pgaptmp_003506"
     CDS             <1..403
                     /gene="nudI"
                     /locus_tag="ID725_RS17530"
                     /old_locus_tag="pgaptmp_003506"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGV7852021.1"
                     /translation="PLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGERIEEALRR
                     EIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINEE
                     FQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            complement(442..1044)
                     /gene="ais"
                     /locus_tag="ID725_RS17535"
                     /old_locus_tag="pgaptmp_003507"
     CDS             complement(442..1044)
                     /gene="ais"
                     /locus_tag="ID725_RS17535"
                     /old_locus_tag="pgaptmp_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGV7852022.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAEHCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1352..2491
                     /gene="arnB"
                     /locus_tag="ID725_RS17540"
                     /old_locus_tag="pgaptmp_003508"
     CDS             1352..2491
                     /gene="arnB"
                     /locus_tag="ID725_RS17540"
                     /old_locus_tag="pgaptmp_003508"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGV7852023.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2495..3463
                     /gene="arnC"
                     /locus_tag="ID725_RS17545"
                     /old_locus_tag="pgaptmp_003509"
     CDS             2495..3463
                     /gene="arnC"
                     /locus_tag="ID725_RS17545"
                     /old_locus_tag="pgaptmp_003509"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGV7852024.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3463..5445
                     /gene="arnA"
                     /locus_tag="ID725_RS17550"
                     /old_locus_tag="pgaptmp_003510"
     CDS             3463..5445
                     /gene="arnA"
                     /locus_tag="ID725_RS17550"
                     /old_locus_tag="pgaptmp_003510"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGV7852025.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            5442..6332
                     /gene="arnD"
                     /locus_tag="ID725_RS17555"
                     /old_locus_tag="pgaptmp_003511"
     CDS             5442..6332
                     /gene="arnD"
                     /locus_tag="ID725_RS17555"
                     /old_locus_tag="pgaptmp_003511"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGV7852026.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            6332..7984
                     /gene="arnT"
                     /locus_tag="ID725_RS17560"
                     /old_locus_tag="pgaptmp_003512"
     CDS             6332..7984
                     /gene="arnT"
                     /locus_tag="ID725_RS17560"
                     /old_locus_tag="pgaptmp_003512"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGV7852027.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            7981..8316
                     /gene="arnE"
                     /locus_tag="ID725_RS17565"
                     /old_locus_tag="pgaptmp_003513"
     CDS             7981..8316
                     /gene="arnE"
                     /locus_tag="ID725_RS17565"
                     /old_locus_tag="pgaptmp_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGV7852028.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            8316..8702
                     /gene="arnF"
                     /locus_tag="ID725_RS17570"
                     /old_locus_tag="pgaptmp_003514"
     CDS             8316..8702
                     /gene="arnF"
                     /locus_tag="ID725_RS17570"
                     /old_locus_tag="pgaptmp_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGV7852029.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8696..8962)
                     /gene="pmrD"
                     /locus_tag="ID725_RS17575"
                     /old_locus_tag="pgaptmp_003515"
     CDS             complement(8696..8962)
                     /gene="pmrD"
                     /locus_tag="ID725_RS17575"
                     /old_locus_tag="pgaptmp_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGV7852030.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(9072..10427)
                     /gene="menE"
                     /locus_tag="ID725_RS17580"
                     /old_locus_tag="pgaptmp_003516"
     CDS             complement(9072..10427)
                     /gene="menE"
                     /locus_tag="ID725_RS17580"
                     /old_locus_tag="pgaptmp_003516"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGV7852031.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(10424..11386)
                     /gene="menC"
                     /locus_tag="ID725_RS17585"
                     /old_locus_tag="pgaptmp_003517"
     CDS             complement(10424..11386)
                     /gene="menC"
                     /locus_tag="ID725_RS17585"
                     /old_locus_tag="pgaptmp_003517"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGV7852032.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(11386..12243)
                     /gene="menB"
                     /locus_tag="ID725_RS17590"
                     /old_locus_tag="pgaptmp_003518"
     CDS             complement(11386..12243)
                     /gene="menB"
                     /locus_tag="ID725_RS17590"
                     /old_locus_tag="pgaptmp_003518"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGV7852033.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12258..13016)
                     /gene="menH"
                     /locus_tag="ID725_RS17595"
                     /old_locus_tag="pgaptmp_003519"
     CDS             complement(12258..13016)
                     /gene="menH"
                     /locus_tag="ID725_RS17595"
                     /old_locus_tag="pgaptmp_003519"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGV7852034.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13013..14683)
                     /gene="menD"
                     /locus_tag="ID725_RS17600"
                     /old_locus_tag="pgaptmp_003520"
     CDS             complement(13013..14683)
                     /gene="menD"
                     /locus_tag="ID725_RS17600"
                     /old_locus_tag="pgaptmp_003520"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGV7852035.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14772..16067)
                     /gene="menF"
                     /locus_tag="ID725_RS17605"
                     /old_locus_tag="pgaptmp_003521"
     CDS             complement(14772..16067)
                     /gene="menF"
                     /locus_tag="ID725_RS17605"
                     /old_locus_tag="pgaptmp_003521"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGV7852036.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16146..16451)
                     /gene="elaB"
                     /locus_tag="ID725_RS17610"
                     /old_locus_tag="pgaptmp_003522"
     CDS             complement(16146..16451)
                     /gene="elaB"
                     /locus_tag="ID725_RS17610"
                     /old_locus_tag="pgaptmp_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGV7852037.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16506..16967)
                     /locus_tag="ID725_RS17615"
                     /old_locus_tag="pgaptmp_003523"
     CDS             complement(16506..16967)
                     /locus_tag="ID725_RS17615"
                     /old_locus_tag="pgaptmp_003523"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGV7852038.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            17032..17949
                     /gene="rbn"
                     /locus_tag="ID725_RS17620"
                     /old_locus_tag="pgaptmp_003524"
     CDS             17032..17949
                     /gene="rbn"
                     /locus_tag="ID725_RS17620"
                     /old_locus_tag="pgaptmp_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGV7852039.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            18137..19360
                     /locus_tag="ID725_RS17625"
                     /old_locus_tag="pgaptmp_003525"
     CDS             18137..19360
                     /locus_tag="ID725_RS17625"
                     /old_locus_tag="pgaptmp_003525"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HGV7852040.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATK
                     HPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFW
                     DKNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWP
                     KEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSF
                     NSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQMES
                     NSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLENRNCYLDKYGDANINHYYRNLEI
                     KNSHPKNRASSKRVS"
BASE COUNT         4809 a         5129 c         4820 g         4791 t
ORIGIN      
        1 ccctttgatt caaaatgatg gtgcttattt gctgtgtaaa atggccgacg atcgcggcgt
       61 tttccccggt caatgggcgc tttcgggtgg cggcgtggag cccggcgaac gaattgaaga
      121 ggctctacgc cgcgaaattc gcgaagaact gggagaacag ctgcttttga cagaaatcac
      181 gccctggacc ttcagcgatg atattcgcac caagacgtat gcagatggtc gcaaggaaga
      241 aatttatatg atttacctga tttttgattg cgtttctgcc aaccgagaag tgaaaataaa
      301 cgaagagttt caggactacg cgtgggtaaa acctgaagat ctggcgcact atgatttgaa
      361 tgtcgccacc cgaaaaacgt tacgtttgaa aggtcttctg taacaatgat gacagccatt
      421 actggctgtc agattgaaaa tttagtgatt aacgaattcc ccatccagat aaactttgcc
      481 tttttccaca tgcatgacta aaccatccag ataatcaggt ttaaatgtcg catcacgctt
      541 atctttagca atatatgtca ggcaatgatt atgggtgaaa ataacgatat ttttatcagg
      601 cgctttgctt tgtaagtcct taatcgcact ataaatctca ttaccgcact gaagaagtcg
      661 tttatctacc gtcaattttt tacccgctga aaaccaggtc gcggactgaa tggtccggac
      721 ggtattactg gaataaagat cgaaatcagg gatatcagca ctaaaagcgt tgcccagttc
      781 acgggcatcc tgggtacctt taaccgtaat acctgtttta tctgacaagc attgattggt
      841 tgaacgatcg caatgttcag catggcgaaa caaaacgaca accgggtgct gctgtgccag
      901 tctgcccagc gttttattgt cgatacgtgg caaaccattg ctactccagg cggcatgagt
      961 acccagtccg gcaattgcag cgagcgccag taaaatgatg aaatattttt ttgacttcaa
     1021 tgatgagcgg caaaaagcta acacgtaaac tccacctata gacaagcgca accagacaat
     1081 taccgtgaaa ttgagcgaca tttctggcga taattagcag ttggtgtaat attaaaaatc
     1141 ctacgatgct ggcaaaatgc ctcaaaattt tgccaaatgc aaagcctaaa taagaaaaaa
     1201 tataaaaatt tcaatattta cgtctaatat tagtttctta aggttaagtt aatattctat
     1261 ccttaaaatt tcgctctaaa tggcaaaata tacacaacac tctttatagc aaatataagt
     1321 ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa tttttgcctt tttcgagacc
     1381 agcaatgggc gtggaggaac tcgctgcagt taaagaggtt ctcgaatccg gttggatcac
     1441 aaccggtccg aagaatcacg cgcttgaaca agccttttgc cagttgacgg gaaatcagca
     1501 tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc acgctaatgg cgttggaaat
     1561 tggcaagggc gatgaagtga ttacgccttc cctgacctgg gtttcaaccc tcaatatgat
     1621 ttccttgcag ggtgcaacgc cggtaatggt ggatgtcgat cgcgatacgc tgatggtcac
     1681 gcctgaagcg atcgaatcag ccattacgcc acgcactaaa gccatcattc cggtgcatta
     1741 tgccggtgcg ccagcagata ttaacgccat tcgcgccatt ggcgaacgtt acggcatcgc
     1801 agttatcgaa gatgctgccc atgccgtagg tacgtattac aaagggcgac atattggcgc
     1861 aaaaggtacc gctatttttt catttcatgc cattaaaaat attacctgtg cggaaggagg
     1921 cctgattgtt actgataatg aaaatattgc ccgccagcta cggatgctga aatttcacgg
     1981 cctgggtgtc gatgcctatg acagacaaac ctggggccgt gcaccgcagg ctgaagtctt
     2041 aacgccgggc tataagtaca atctgaccga tattaacgcc gcgattgccc tgacacagtt
     2101 agccaaatta gagcacctca acacccgtcg gcgcgaaatt gcccagcaat atcagcaagc
     2161 actggcagct ctcccctttc agccattaag ccttcccgcc tggccgcacg ttcacgcctg
     2221 gcatctgttt attattcgtg tcgatgaaca acgttgtggt atcagtcgcg atgcgttgat
     2281 ggaagcgtta aaagaaagag gtattggtac cgggttacat ttccgcgccg ctcacacaca
     2341 aaaatattat cgcgagcgtt ttccctcgct gtcgttaccg aataccgaat ggaatagcga
     2401 acgcatctgt tctttgccgc tgttcccgga tatgactacc gccgatgccg accgtgtcat
     2461 cacagccctt cagcaactcg caggacaata agccatgttt gaaatccacc ctgttaagaa
     2521 agtctcggtg gttattcccg tttataacga gcaggaaagc ttaccggaat taatcaggcg
     2581 caccaccaca gcctgtgaat cgttggggaa agagtatgag atcctgctga ttgatgacgg
     2641 cagtagcgat aattccgcgc atatactggt cgaagcctca caagcggaga acagccatat
     2701 tgtatctatt ttgcttaacc gcaattacgg gcaacattca gcgattatgg cgggattcag
     2761 tcacgttact ggcgacttaa ttattaccct tgatgccgat ctccagaatc cgccagaaga
     2821 aatcccccgc ctggtggcaa aagccgatga aggttacgac gtggtaggga ctgtacgcca
     2881 gaaccgccag gacagctggt ttcgtaaaac agcttcgaag atgattaacc ggcttattca
     2941 gcgcaccacc ggcaaagcga tgggtgacta cggttgtatg ctgcgcgcct atcgccgtca
     3001 tattgtcgat gcgatgttgc actgccatga acgcagcacc tttatcccga ttctggcgaa
     3061 tatcttcgcc cgccgtgcca ttgaaattcc agtgcatcat gccgagcgtg agtatggtga
     3121 atccaaatac agctttatgc gcctgattaa tttgatgtac gacctggtga cctgccttac
     3181 cacaacgccg ctacgtatgc tgagcctgct cggcagcatt attgcgattg gaggttttag
     3241 cattgcggtg ctgctggtga ttttacgcct gaccttcgga ccacaatggg cggcagaagg
     3301 cgtctttatg ctatttgccg tgctgtttac ttttattggc gctcagttta tcggcatggg
     3361 attactcggt gaatatatcg gcaggatcta caccgatgtc cgcgcccgcc cccgctattt
     3421 tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa taatgaaaac cgtcgttttt
     3481 gcctaccacg atatgggatg cctcggtatt gaagccctgc tggctgccgg ttacgaaatt
     3541 agcgccattt ttacccatac cgataatccc ggtgaaaaag ccttttatgg ttcggtggct
     3601 cgtctggcgg cggaaagagg cattccggtt tatgcgccgg ataacgttaa tcatccgctg
     3661 tgggtggaac gcattgccca actctcacca gaggtgattt tctcttttta ttatcgccat
     3721 cttatttgcg acgagatttt gcagctcgct cccgcaggtg catttaatct gcacggttcg
     3781 ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg tgctggtgaa cggtgaaaca
     3841 gaaactggcg ttacattgca ccgaatggta aaacgtgccg atgccggggc cattgtggcg
     3901 caactgcgcg ttgccattgc gccagacgat atcgctatta cgctgcatca taaattgtgc
     3961 catgccgcgc gccagctact ggaacagaca ttacccgcca ttaaacacgg taatattctg
     4021 gaaatcgccc agcgcgaaaa cgaagccacc tgttttggtc gcagaacgcc ggatgacagt
     4081 ttcctcgaat ggcataagcc ggcatccgta ctgcacaaca tggtacgtgc cgttgccgat
     4141 ccgtggccgg gtgccttcag ctatgttggc aatcagaaat tcaccgtctg gtcgtcgcgt
     4201 gttcatcctc atgccagcaa agcacagccg gggagcgtga tttctgttgc gccactgctg
     4261 attgcctgtg gcgatggcgc gctggaaatc gtcaccggac aagcgggcga cggcattact
     4321 atgcagggct cgcaattagc gcagacgctg ggcctggtgc aaggttcacg cttgaatagc
     4381 cagcctgcct gcaccgcccg acgccgtacc cgggtactca tcctcggggt gaatggcttt
     4441 attggcaacc atctgacaga acgcctgctg cgcgaagatc attatgaagt ttacggtctg
     4501 gatattggca gcgatgcgat aagccgtttt ctgaatcatc cgcattttca ctttgtcgaa
     4561 ggcgatatca gtattcattc cgaatggatt gagtatcacg tcaaaaaatg tgatgtcgtc
     4621 ttgccgctgg tggcgatagc cacgccgatt gaatataccc gcaacccgct gcgcgtattt
     4681 gaactcgatt ttgaagagaa tctgcgcatt atccgctact gcgtgaagta ccgtaagcga
     4741 atcatcttcc cgtcaacttc agaagtttat gggatgtgta gcgataaata cttcgatgag
     4801 gaccattcta atttaatcgt cggcccggtg aataaaccac gctggattta ttcggtatca
     4861 aaacaattac ttgatcgggt gatctgggcc tatggcgaaa aagagggttt acagttcacc
     4921 ctcttccgcc cgtttaactg gatgggacca cgactggata accttaatgc ggcacgaatc
     4981 ggcagctccc gcgcgattac gcaactcatt ctcaatctgg tagaaggttc accgattaag
     5041 ctgattgatg gcggaaaaca aaaacgctgc tttactgata ttcgcgatgg tatcgaggcg
     5101 ttataccgca ttatcgaaaa tgcgggaaat cgctgcgacg gcgaaattat caacattggc
     5161 aatcctgaga acgaagcgag cattgaggaa ctgggcgaga tgctgctggc gagcttcgaa
     5221 aaacatccgc tgcgccatca tttcccaccg tttgcgggct ttcgcgttgt cgaaagtagc
     5281 agctactacg gcaaaggata tcaggacgta gagcatcgta aaccgagcat ccgcaatgcc
     5341 caccactgcc tggactggga gccgaaaatt gatatgcagg aaaccatcga cgaaacgctg
     5401 gatttcttcc tgcgcaccgt tgatcttacg gataaaccat catgaccaaa gtaggcttac
     5461 gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt gccgcgtctg ctggaaatct
     5521 tgagtaagca taatattcag gccagcattt ttttcagcgt cggcccggac aatatgggcc
     5581 gccatctctg gcgactggtg aagccacagt ttttgtggaa gatgctgcgc tcaaacgcgg
     5641 catcgcttta tggctgggat attttactgg caggtacggc ctggccaggt aaagagattg
     5701 gtcatgccaa tgccgatatc attcgtgaag cggctaaata tcacgaagtc ggcctgcacg
     5761 cctgggatca ccatgcctgg caagcccgta gcggtaactg ggatcggcaa acaatgatcg
     5821 acgatattgc acgcggcctt cgcactctgg aagagattat cggtcaaccg gtaacctgtt
     5881 ctgccgctgc gggctggcgt gccgaccagc aggtgatcga agcaaaagaa gcgttccatt
     5941 tgcgctacaa cagcgattgt cgtggggcca tgccgttccg tccactgctc gaatcaggaa
     6001 accctggcac tgcgcaaatt ccggtgacct tacccacctg ggatgaagtg attggtcggg
     6061 atgtgaaagc agaagatttt aacggttggt tactcaaccg catcctgcga gataaaggca
     6121 cgccggttta taccattcat gcagaagttg aaggctgcgc ttatcagcat aattttgtgg
     6181 atctcctcaa acgcgcagct caggaaggcg tgacattttg ccctttaagc gaactgttat
     6241 cagagacgtt gccgctcgga caagttgttc gcggaaatat tgccggacgt gaaggctggc
     6301 tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg gtacgttacc ttatcggcct
     6361 cttcgcgttt attgcctgct attacctgtt accgatcagc acgcgtctgc tatggcaacc
     6421 cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg gcatccggcg actggattgt
     6481 gccccatctg ttagggctac gttattttga aaaacccatt gccggatact ggattaacag
     6541 cattgggcaa tggctatttg gcgcgaataa ctttggtgtg cgggcaggcg ttatctttgc
     6601 gaccctgtta actgccgcgc tggtgacctg gtttactctg cgcttatggc gcgataaacg
     6661 tctggctcta ctcgccacag taatttatct ctcattgttt attgtctatg ccatcggcac
     6721 ttatgccgtg ctcgatccgt ttattgcctt ctggctggtg gcgggaatgt gcagcttctg
     6781 gctggcaatg caggcacaga cgtggaaagg caaaagcgca ggatttttac tgctgggaat
     6841 cacctgcggc atgggggtga tgaccaaagg ttttctcgcc cttgccgtgc cggtattaag
     6901 cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat ctctttattt acggctggct
     6961 ggcggtcatc agttgcgtac tgacggttct cccctgggga ctgacgatag cgcagcggga
     7021 gcctgacttc tggcactatt ttttctgggt tgagcatatt caacgctttg cactggatga
     7081 tgcccaacat agagctccgt tctggtacta cgtgccggtc atcattgccg gtagtctgcc
     7141 gtggctggga ttactccccg gtgcactata cacaggctgg aaaaaccgca agcattccgc
     7201 aaccgtctat ttgttgagct ggacgataat gccgctgctg tttttctccg ttgctaaggg
     7261 taaattgccc acctatattc tttcctgctt tgcacctttg gcaatgctga tggcgcatta
     7321 cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg attaatggct ggattaacat
     7381 cgcttttggc gtcactggca ttattgccac atttgtggtc tccccctggg gaccaatgaa
     7441 cacgccggtg tggcaaacct tcgagagcta taaagtcttt tgtgcctggt cgattttttc
     7501 gctatgggca tttttcggct ggtacacctt aacaaacgtc gaaaagacct ggtcttttgc
     7561 cgcgctttgc ccgctggggc tggcgttgct ggtaggattt tcaattcctg acagagtcat
     7621 ggaaggaaaa catccgcaat tttttgtcga gatgacacaa gaatcacttc agccaagccg
     7681 ctatattctt actgatagcg tcggtgttgc cgcaggtctg gcatggagtc tgcaacgcga
     7741 tgacatcatc atgtatcgcc agacaggtga gttgaaatac ggccttaatt atccggatgc
     7801 gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg cttaatcaac atcgtcagga
     7861 ggggattatt acactcgtgc tttcggttga ccgcgatgaa gatatcaaca gtctcgccat
     7921 tccgcccgca gatgccatcg atcgtcagga gcgtctggtg ctgattcagt atcgtcccaa
     7981 atgatctggc taacattagt ctttgccagc ttgcttagcg ttgccgggca gttgtgtcag
     8041 aaacaggcaa cctgctttgt ggcgataagc aaacggcgca aacatatcgt gctgtggctg
     8101 ggactggcgc tggcttgtct tggtcttgcc atggtgctct ggctgctggt cttgcagaac
     8161 gtaccggtag gtattgccta cccgatgtta agtctgaatt ttgtctgggt gacgctggct
     8221 gcagtaaaac tgtggcacga accggtatcg ccgcgtcact ggtgtggggt ggcgttcatt
     8281 attggtggca ttgtgatcct cgggagtacg gtgtaatggg cctgatatgg ggattattca
     8341 gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt tgcggcgagt catctgccgc
     8401 cgatgacgca cttatgggat tttatcgcgg cgctgttagc ttttgggctg gatgcccgga
     8461 tactgttact cgggctgctg ggttatctgc tttccgtttt ttgttggtat aaaacactac
     8521 ataaacttgc gctaagcaaa gcctatgcct tgctcagtat gagttatgtg ctggtatgga
     8581 ttgcttcaat ggtacttccc ggctgggaag ggactttttc gctgaaagca ctactgggag
     8641 tagcctgtat tatgagcggg ttgatgctga ttttcctgcc cacgacaaaa caacgttact
     8701 gagttttccc tgccacttta cactgccgtt cccactcatc ggggctataa cagcttgcag
     8761 agaggacttt caccgtatgc agcttttcac gattaataca ataaagcgca ttctgcaaag
     8821 gcgagagtaa atcccccact ttcacggcga agtcactctt cacttcggcg atcattttta
     8881 tcgcgccgcc agcatcgcag agcataagta catgtctgtt atcttgttta ttgcagcacg
     8941 attttttgac cagccattcc attgtattat cctgtttgct aagagttttc atatcaattt
     9001 acagattaac agataaactc tttatcaacg gcaatggaat ggcagaactg agaagtgaaa
     9061 cggagggtta attattgctg acgttgcacc cactctttta gcgcctgacg tgaaatttta
     9121 ataccgccgt ttttcagctc cggcggcaaa gtcagccaac gtaccggttg ctgaaaacgg
     9181 gccagcttat ctttcaccca ttcactaaga tcaacgctct cgtggtcata ctccatcacc
     9241 gccaccggtc gatgaccaaa ctccttgtcg gcaacgggga cgataaacac ctgcaaaacc
     9301 gcaggatgtg cagcaattac gcgctcgact tcttccgtct gaataccctc tccgccactg
     9361 aagaatagat tgtctaaacg tccgacaatg gtcagcttgc cattatgcat ctcaccgcga
     9421 tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa ccagttgccc gttacgccag
     9481 taaccttctg ccatactggc agcccgcagc cacacttcat tattaacgat tttcacttcc
     9541 cgacccggca gcggcgaacc aacgtctgcc aggccgtcgg cttctttcgc acacaccgtg
     9601 gaggcaaact cggtcagacc atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt
     9661 tccgtcaact cgaccgggat agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa
     9721 ctgcggttaa ccagcaaacg ccagagttgt gttggcacca gtgaagcgtg agtacagcct
     9781 gccagcattt gctccaatgg ctgtttatca cgcaccgtca tccgcgcacc agcgtataac
     9841 cagcgccaca taattccctg accggagacg tgaaacagcg gtaaagagag caaccagtca
     9901 tcgtgatcgc caaacggaat cagcgataac accccttcgg cactggcaag atgggcctga
     9961 taagtatgga cagcggcttt cggcaacccg gtagaacccg aggtcaacgt cattgagcac
    10021 agacgcgtcg gctgccacgt agcgacatgt gcgccttcaa cccgctgaat gtgcagcgac
    10081 gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg caaattgcag cgtcagattg
    10141 ggcagcaatt cttcaagcaa cggttgcggc agctgagggt tcacgggcaa cacccgcgcc
    10201 ccgcattgca gtaacgccag ccaagcgagc agcgtttgcg gcgtattcca cgcccgcaac
    10261 atcacgccgc tgtcctcaac cacaccctgc accgcaaatc cggaggctaa ttcatcgacg
    10321 cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa gacgtaaggc gatggcttct
    10381 ccccgcactt gccgccagtg acgccacggc cagtcagaga agatcataac aaccgctcca
    10441 gtgcatcaac ttccacgaca ggcagcgtgc tacccggcca gcgacgtacc tgctgcgcct
    10501 gcattagatc cagcgtgtcc agccctggaa tggtgtccgg cgttaaccag gcagcaatcc
    10561 gcgccagttg cgttaagcct aagctcgatt caatggaaga actgatcact gccgtcagcc
    10621 ccagcgcgtg cgccgcctgt acctgctcgc gaactttctc cagactgccc gtgagcgtgg
    10681 gtttgataac taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca
    10741 agctttcatc ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc
    10801 gggttttgca cggctcttcg agaaacgcga tgcggtggcg ataatccggg ttaacgtatt
    10861 tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca
    10921 gatccggaat tgcctccagc aacagattca ccaccatgcc gtcgcgcacc gcttcgtaca
    10981 atcccacttt gaccttcgcc actttctcgc ctggcatatc tgcaagtttg aggatcagat
    11041 cgtccggatc gccattacac agtggtgccg tacggtaatt ggccgcctgc gggagcgtat
    11101 ctgccagctc cgccaatgca caacttacgc caaaggcaac ggaaggcatc tgcggtaatt
    11161 cgcaatcgcc tgccagccag ttatttaccc aggcaagcag cacactttgc gcctcttccc
    11221 aggtttcctg actgaagccc ggcagtgggg agatctcccc ccacccttcg cgctcgccct
    11281 cacgcaggca aacgtacagc ccgtcgcggg tttttaaccg cctgtcgcgc agaaccaccc
    11341 ccgcgtccat ggggatctgc cagcggtata cctgcgcgct acgcattacg gattccgttt
    11401 gaatttgctg aagtcaggct gacgtttctg gttgaaggcg ttgcgacctt cctgaccttc
    11461 ttccgtcatg tagaacagca tggtggcgtt gcccgccagc tcctgcagac ctgcctgacc
    11521 gtcacagtcg gcgttcagtg cagctttcag gcagcgcagc gccatcggac tgttttgcag
    11581 catttcacgg caccagcgaa cggtttcttt ttccagatcc gccagcggta ccacggtgtt
    11641 gacaaggccc atatccagcg cctgttttgc gtcgtactga cggcacagga accagatttc
    11701 acgcgctttt ttctgcccga cgatacgagc catgtaggaa gcgccccagc cgccgtcgaa
    11761 ggaaccgact ttcgggccag tctgaccgaa gatggcatta tctgccgcga tagtcaggtc
    11821 gcacatcatg tgcagaacgt gaccaccgcc gatggaatag ccagccacca tcgcgacaac
    11881 cggtttcgga caggtacgga tctgacgctg gaagtccagc acgttcaggt gatgtacgcc
    11941 ggaatcatct ttatagccgc cgtaatcacc acgcactttc tggtcaccac cggagcagaa
    12001 cgctttatcg cctgcgccag tcagaatgat cacgccgacg ttatcgtcat aacgcgcatc
    12061 tgccagtgcc tggatcatct cttttaccgt cagaggacgg aaggcattgc gcacctgcgg
    12121 acgattaatg gtgatttttg cgataccgtc ggtggatttt tcataacgaa tgtcctcaaa
    12181 gccttcggag cagtcgtgcc attcaaccgg tgcgtaaagc attgcttcat caggataaat
    12241 catattgtgt cctttagtca gaaacgcaag atctgcgcca gacttgcgat tacgccagcg
    12301 ggattttccc gatgcgcgtt atgtccggcg cgaggaatga catggcagtc ggcagccagt
    12361 tccgccgcca gggcgcggaa tttgctgtca cgttcaccac ataaataata aaacgcaaat
    12421 gtgcgggcgc taaggttagc acgtaaatca ggctggacgg cgagagaagt cgcctccagc
    12481 atggcggcaa gggttgcgcc attattgttg ctgcgcagca ccaccagctc ccggcgttga
    12541 tcgtcattga gcgaggcaaa aacaggctgt tgataccagt cggcaaatac cgcggttaat
    12601 ggttcggaac gaaaacgctg cgcccattgg cgatcggaac gctgacgttc cgcgcgttgt
    12661 tcagcatttt gcagccccgg atgaccgcct tcgacaacaa ccccacaaag ccccgccagc
    12721 ccctggcaag cggccatcat cgccacccgt ccgccaagcg agtaccccac cagccagaag
    12781 ctaaggatgt tgtaactaac cagggtttta cgcagtaagt cggtgacatc atcaaatcca
    12841 tcgacgctaa tcgccgccga accaccgtga cctgggagat caacatacaa ccgtgagtag
    12901 tcggcaaacg cctcgccgac ttcctgccat tcgaggcaat cgccggaaaa accgtgcaaa
    12961 aacaccagcc agggtaaacc tggtttgccg tgttttgcct gcgcgtgcag gatcataaat
    13021 ggcttacctg cgccagcagc tgctggagcg tttgcgcgcc gtcggtgtcg ttaaccacca
    13081 tttcaattac cgtggtggtc ggagtacgcc aggcgtcggc aagtaccgtt tcaagttcct
    13141 gccagttttg cggacgatga tatttcagct caaacatcgc ggcagcgtgc tcaaaatgga
    13201 cgttttgcgg catcagatag aaacgctcgc gctcgctttt cggcgttggc agcagcgaga
    13261 aaatttgccc gccgttgttg ttcaccacaa ttaataccag cggcgcggaa acctggcgca
    13321 ataacgccag cgcattcagg tcgtaaagtg cggagagatc gcccacaatc gccagcgtcg
    13381 gtttgccgct tgcccgctga acgccggcgg cagtcgagag cagcccgtcg ataccgctgg
    13441 ccccacggtt gctgtacacc gggtaacctg ccggaagttg cgaaagcgca tcaatcagac
    13501 gtaccaccag gctgttaccg acaaacaatt gcccctgttc aggcagatag tcgctgatgc
    13561 gatgcgccag ttgcgcttcg ccaaacgcat cgcgacaggc aataaccgcc tgcattgcct
    13621 gttccgccag gcgcgggatt tcaacgcacc agggctggcg tttttctgcc ggatgcagtt
    13681 ccagccagtc ggcaatattg gcaattaagc ggcgtccacg atggtgtgcc ggatcaagcc
    13741 gcccttcgat gtcatccaca atccagtact cttcgggttc acagcttgcc tgccattgca
    13801 ggagccgttt gcccgtcagg ctgcttccca gttgcaccac aatttgcgcc tgttgcagtt
    13861 cgctggtcgc tttggcattg cctaaccaga gatcggcaca cggcagcggc tgtccggttt
    13921 gcgacagcac gtcgccaatc agcggccagc caagagtttg cgcccacagg gcaacttttt
    13981 tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc gcgcttttgc cgccagaaga
    14041 accagtcgcg ctgtttttcg ctctcaagac gaggcgcttc acgcagccac ggcttatcat
    14101 cctgccacca gtcacccaga cgctgttgcc agctaagccc agtgtcgtcc atctcgccat
    14161 acagcggttc agcaaacggg cagttgatat ggaccccacc agcatgaagc gtaccgagag
    14221 catggtcgat ggtagaaacc agccaacgtg cggggatatc ctgggtcggg cgcggcagtg
    14281 aaatactgtg cgtggggtga gaggcgaaca ttcccggctg acgaatcgcc tgattcgcgc
    14341 cgcagtcaat tagctccggc gggcgatcgg cggttaacag gatcagtttt tctccggtta
    14401 acccagcttc aatcagtgcc ggatagagat ttgctaccgc cgtgccggag gtcacaatca
    14461 ccgccaccgg ctgcttgctg actttcgcca gccccagcgc cagatgcccc agtccacgct
    14521 catcgaaatg ggtgtggtga atgaatgcgg aattctccgc cgccgctaac gttaacggtg
    14581 tagaacgcga gccaggggcg atgcagatgt gtcttacgcc gtgacgcgtt aatgcttcca
    14641 gaatgaccgc cgcccagcgt cggttaaatg cgcttactga catgagtttg tccggtatca
    14701 atattgcggc taagtataag gagctacaaa aatagattat tgatatgaat cggtaatgat
    14761 gcgactcatt actattccat ttgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt
    14821 tcctgccact cttgctcggg gtcggaacca cggacaatgc ccgcgccagc atatagccgc
    14881 acgacattgc cgctgatttt tgctgagcgc agggaaacgc agaattcgct ttgttgtaat
    14941 gagagatagc ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg atgggcgata
    15001 aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg ccgttggctg caactgatgt
    15061 aaacagatca catcatccgc tttgttgagt gaagtccaga tacagcggcg aagatgctgc
    15121 actttacgca gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt
    15181 aatcgttggc agatatcttc caccaccagc atgttctcgc gctggttttt atcatccgcc
    15241 atcagccact ctcctaactg ctgcgcctgc ttatcatcag gatgatttgc tactgttccc
    15301 gccagcgctt cagtacgcag cgctttgtca cgccgccgcc ataaccgttc cggtgaagag
    15361 ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt gagattcagt
    15421 cgacgactgg cagccatcat cgccgccgca ttgaccggac ttgcgaaatg caggtcagtt
    15481 gcccgagcaa gcaccacttt gtcgagctca ccttcggcga tggttttcgt tgccagttcg
    15541 attaattgcg tccagcccgt tttgtccggc cagtgttgtt ctcgcgtagt ggttaaatgt
    15601 aacccaggca agggcttgat actcaccagt gtggcgataa attctcttgc cttaatcgca
    15661 tcgtgctgaa gggagctttc gctgaatagc gtcagccgca gcgtggcttt accgccacag
    15721 cgtcgccatt ccaggcgtgg taaaagtaaa ttgccctgcg acgggtcaaa cgcattcagc
    15781 ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc
    15841 aacgacgtaa aacgggtaat cgcgcccagg acggcagctt cttcatcacc attacgttgt
    15901 tgccagtaga attgcgggta cgtttgctga ctggccagcc agctcaaggc atcaaaagcg
    15961 tctttgagag ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc
    16021 gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg gacctctccc
    16081 tgttaaaagc ctcacattat acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg
    16141 aggttttaac ggcgtgccag caacagtcct agtaccagcc caacggccgc acccacacca
    16201 attccctgcc agggtttttc gtggacgtag tcatcagcac gataaacagc ctgcttcgcc
    16261 cgatagtaat aactgtctga agcctggcta acccgttttt tcacatcatc cagcgctttt
    16321 tctgcacgcg ctttcagctc aacatatttc tgatcggcgg gatcgccaga ggagcggagc
    16381 acctcttcca gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac
    16441 tgattagaca ttctcgttct ccatttgcgt aaaacctgtc actaactata gacaagggtt
    16501 attgattacg cctgaattac ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca
    16561 tagatctccg tcacagggat aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc
    16621 gccccgaggt agacaggctt atcaggccag tgatgcgtgc aagtgtcgag ggttttactc
    16681 atcagttgct gccccacttt ttcgccacgc aacgcttcac tgacaatcac ccgaccaata
    16741 acgaccggct caagatcatc atcacttttc agaatcctcg catatgccac cagttcatca
    16801 tttttccaac cgagaatatg gcgattatcc cccgtcaaat catcaccatc gatatcctga
    16861 taagggcaat tttgctcgac aacaaacacc gcgcaacgca gttgtaataa ggcataaagc
    16921 tgggaaaccg ataattcaga gtgatgcaga tcttgccatt caatcataat gctctccttt
    16981 tagatgtatg ccttatacta gccggaatat tttttgaaac gtgatgaact catggaatta
    17041 atttttttag gtacttcggc cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg
    17101 ctaaacctgc aacatccgac ccagagcgga ctttggttgt ttgactgcgg tgaaggcacc
    17161 cagcatcagc tactgcatac cgcctttaac cctggaaaac tggacaagat ttttatcagt
    17221 caccttcatg gcgatcatct ttttggttta cccggcttgc tgtgcagtcg ttctatgtca
    17281 ggcattatcc aacccttaac gatttatggt ccgcaaggta tccgtgaatt tgtggagacc
    17341 gcgctgcgga ttagcggctc atggaccgat tatccgctgg aaattgtcga aattggcgct
    17401 ggcgaaattc tcgatgatgg cctgcgcaaa gtgaccgctt atccaatgga acacccactg
    17461 gaatgctatg gctatcgtat tgaagaacat gataaaccgg gtgcattaaa tgcccaggca
    17521 ttaaaagctg ctggcgtacc gcctagccca ctgtttcagg aattaaaagc gggcaaaaca
    17581 atcatgctgg aagatggaag gcagattaac ggcgcagatt acttagctgc tccagtgcca
    17641 ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg atgccgcact tgacctggct
    17701 aaaggtgtcg atgtcatggt gcacgaagcg acgctggata taaccatgga agccaaagcc
    17761 aatagtcgcg gtcatagctc tacacgccag gctgcgacac tggcccgtga ggctggagtc
    17821 ggtaagctaa tcattaccca cgtcagctcg cgctatgatg acaaaggttg tcagcacctg
    17881 ttacgtgaat gcaggtcaat tttcccggcg actgaactag cgaatgattt caccgtgttt
    17941 aacgtttaac tctgcttatt attaaaaaca gggcgaaact tgccctgtta tcgcaacccg
    18001 cgctacatat atccactcac acgggcatat tgagcgattt ttgatattta ttcatctcct
    18061 tacgcactat attcagagca ataagttgcc tctttttatc ttcattcagc acaacctttg
    18121 caactaaaag gagcatatga tggttacagt tgtcagcaat tattgtcaat tatctcaaac
    18181 ccaactcagt cagacatttg cagaaaaatt tactgtgacc gaggaattac tgcagtcttt
    18241 aaaaaaaaca gcgttatccg gagatgaaga aagcattgag ttactgcata atattgcgtt
    18301 aggttatgat gaatttggga aaaaagctga agatattctt taccatattg ttagaaaccc
    18361 aacaaatgat accctatcga ttatcaagct tataaaaaat gcctgtttaa aattatataa
    18421 tttggcacat accgcaacca aacaccccct caaatcacat gattcagata atctcctgtt
    18481 caaaaaacta ttctcccctt cgaaattaat ggcaattatc ggtgaggaca ttcctctcat
    18541 atcagaaaaa cagtcacttt caaaggtact tttaaatgat aagaataatg aactgagcga
    18601 tgggacaaac ttctgggata aaaatcgtca attaaccaca gatgaaatag cttgctatct
    18661 taaaaagatc gccgccaatg caaaaaatac tcaagtcaat tatcctactg atttctacct
    18721 ccccaattcc aacagcactt acctggaagt cgctctcaat gataatatta agagcgatcc
    18781 atcatggccg aaagaagtcc agttattccc cataaatact ggcggacact ggatattagt
    18841 ttcgctacag aaaatagtta atgaaaaaaa caacacacaa caaataaaat gcatcatatt
    18901 caattcatta cgtgcactag gccatgaaaa agaaaattca cttaagcgta tcattaacag
    18961 tttcaatagt ttcaattgtg accccacgag agaaacgccg aataataaga atataacaga
    19021 tcatttaact gaaccagaga taatattttt acatgccgat cttcagcaat acttaagcca
    19081 aagttgcggt gcatttgtgt gcatggcagc ccaggaagtg attgaacaaa tggaaagcaa
    19141 ttctgacagc gccccctata cgttattaaa aaactatgct gacagattta aaaaatattc
    19201 agcagaagag cagtacgaaa ttgattttca acatcgtctg gaaaacagaa attgttattt
    19261 agataaatat ggcgatgcaa atatcaatca ttattataga aacttagaaa taaaaaactc
    19321 acaccccaaa aatagagcat caagcaaaag agtaagttaa catcattgtt aattaattcg
    19381 accagtcagc aaatatgctg actggtcgaa catagtttgc taaaaagtca tcactgactg
    19441 atgtcagtca caccacccgc cagttcaatc ttcgggtatt tccagcgtat ctcggcacgc
    19501 gattgctggt ctgttgatgt agtggattgt tgttgctgac ttttcttac
//
LOCUS       DABSYT020000083        19453 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.083, whole genome shotgun sequence.
ACCESSION   DABSYT020000083 DABSYT020000000
VERSION     DABSYT020000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.083"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(125..501)
                     /gene="rnpB"
                     /locus_tag="ID725_RS17630"
                     /old_locus_tag="pgaptmp_003526"
     ncRNA           complement(125..501)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ID725_RS17630"
                     /old_locus_tag="pgaptmp_003526"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(534..1679)
                     /gene="garK"
                     /locus_tag="ID725_RS17635"
                     /old_locus_tag="pgaptmp_003527"
     CDS             complement(534..1679)
                     /gene="garK"
                     /locus_tag="ID725_RS17635"
                     /old_locus_tag="pgaptmp_003527"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGV7852041.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1776..2666)
                     /gene="garR"
                     /locus_tag="ID725_RS17640"
                     /old_locus_tag="pgaptmp_003528"
     CDS             complement(1776..2666)
                     /gene="garR"
                     /locus_tag="ID725_RS17640"
                     /old_locus_tag="pgaptmp_003528"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGV7852042.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2696..3466)
                     /gene="garL"
                     /locus_tag="ID725_RS17645"
                     /old_locus_tag="pgaptmp_003529"
     CDS             complement(2696..3466)
                     /gene="garL"
                     /locus_tag="ID725_RS17645"
                     /old_locus_tag="pgaptmp_003529"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGV7852043.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKTIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3482..4816)
                     /gene="garP"
                     /locus_tag="ID725_RS17650"
                     /old_locus_tag="pgaptmp_003530"
     CDS             complement(3482..4816)
                     /gene="garP"
                     /locus_tag="ID725_RS17650"
                     /old_locus_tag="pgaptmp_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGV7852044.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5191..6762
                     /gene="garD"
                     /locus_tag="ID725_RS17655"
                     /old_locus_tag="pgaptmp_003531"
     CDS             5191..6762
                     /gene="garD"
                     /locus_tag="ID725_RS17655"
                     /old_locus_tag="pgaptmp_003531"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGV7852045.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6911..7246
                     /gene="prlF"
                     /locus_tag="ID725_RS17660"
                     /old_locus_tag="pgaptmp_003532"
     CDS             6911..7246
                     /gene="prlF"
                     /locus_tag="ID725_RS17660"
                     /old_locus_tag="pgaptmp_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGV7852046.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7246..7710
                     /gene="yhaV"
                     /locus_tag="ID725_RS17665"
                     /old_locus_tag="pgaptmp_003533"
     CDS             7246..7710
                     /gene="yhaV"
                     /locus_tag="ID725_RS17665"
                     /old_locus_tag="pgaptmp_003533"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGV7852047.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(7765..8574)
                     /gene="agaR"
                     /locus_tag="ID725_RS17670"
                     /old_locus_tag="pgaptmp_003534"
     CDS             complement(7765..8574)
                     /gene="agaR"
                     /locus_tag="ID725_RS17670"
                     /old_locus_tag="pgaptmp_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGV7852048.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8823..10103
                     /gene="kbaZ"
                     /locus_tag="ID725_RS17675"
                     /old_locus_tag="pgaptmp_003535"
     CDS             8823..10103
                     /gene="kbaZ"
                     /locus_tag="ID725_RS17675"
                     /old_locus_tag="pgaptmp_003535"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGV7852049.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10126..10599
                     /gene="agaV"
                     /locus_tag="ID725_RS17680"
                     /old_locus_tag="pgaptmp_003536"
     CDS             10126..10599
                     /gene="agaV"
                     /locus_tag="ID725_RS17680"
                     /old_locus_tag="pgaptmp_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGV7852050.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            10610..11389
                     /gene="agaW"
                     /locus_tag="ID725_RS17685"
                     /old_locus_tag="pgaptmp_003537"
     CDS             10610..11389
                     /gene="agaW"
                     /locus_tag="ID725_RS17685"
                     /old_locus_tag="pgaptmp_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGV7852051.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11379..12257
                     /gene="agaE"
                     /locus_tag="ID725_RS17690"
                     /old_locus_tag="pgaptmp_003538"
     CDS             11379..12257
                     /gene="agaE"
                     /locus_tag="ID725_RS17690"
                     /old_locus_tag="pgaptmp_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGV7852052.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12275..12709
                     /gene="agaF"
                     /locus_tag="ID725_RS17695"
                     /old_locus_tag="pgaptmp_003539"
     CDS             12275..12709
                     /gene="agaF"
                     /locus_tag="ID725_RS17695"
                     /old_locus_tag="pgaptmp_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGV7852053.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITSTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12706..13839
                     /gene="nagA"
                     /locus_tag="ID725_RS17700"
                     /old_locus_tag="pgaptmp_003540"
     CDS             12706..13839
                     /gene="nagA"
                     /locus_tag="ID725_RS17700"
                     /old_locus_tag="pgaptmp_003540"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGV7852054.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14190..15344
                     /gene="agaS"
                     /locus_tag="ID725_RS17705"
                     /old_locus_tag="pgaptmp_003541"
     CDS             14190..15344
                     /gene="agaS"
                     /locus_tag="ID725_RS17705"
                     /old_locus_tag="pgaptmp_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGV7852055.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15357..16217
                     /gene="kbaY"
                     /locus_tag="ID725_RS17710"
                     /old_locus_tag="pgaptmp_003542"
     CDS             15357..16217
                     /gene="kbaY"
                     /locus_tag="ID725_RS17710"
                     /old_locus_tag="pgaptmp_003542"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGV7852056.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16384..16860
                     /gene="agaB"
                     /locus_tag="ID725_RS17715"
                     /old_locus_tag="pgaptmp_003543"
     CDS             16384..16860
                     /gene="agaB"
                     /locus_tag="ID725_RS17715"
                     /old_locus_tag="pgaptmp_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGV7852057.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            16932..17702
                     /gene="agaC"
                     /locus_tag="ID725_RS17720"
                     /old_locus_tag="pgaptmp_003544"
     CDS             16932..17702
                     /gene="agaC"
                     /locus_tag="ID725_RS17720"
                     /old_locus_tag="pgaptmp_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGV7852058.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17692..18483
                     /gene="agaD"
                     /locus_tag="ID725_RS17725"
                     /old_locus_tag="pgaptmp_003545"
     CDS             17692..18483
                     /gene="agaD"
                     /locus_tag="ID725_RS17725"
                     /old_locus_tag="pgaptmp_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGV7852059.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18484..19239
                     /gene="agaI"
                     /locus_tag="ID725_RS17730"
                     /old_locus_tag="pgaptmp_003546"
                     /pseudo
     CDS             18484..19239
                     /gene="agaI"
                     /locus_tag="ID725_RS17730"
                     /old_locus_tag="pgaptmp_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT         4704 a         5058 c         4956 g         4735 t
ORIGIN      
        1 ttacgtaaat caggtgaaac tgaccgataa gccgctttct tttgggtata gtgtcgtgga
       61 cagtcattca tctttctgcc cctccaaaag caaaaacccg ccgaagcggg tttttacgta
      121 aatcaggtga aactgaccga taagccgggt tctgtcgtgg acagtcattc atctaggcca
      181 gcaatcgctc actggctcaa gcagcctacc cgggttcagt acgggccgta ccttatgaac
      241 ccctatttgg ccttgctccg ggtggagttt accgtgccac ggactgttac cagccgcgcg
      301 gtgcgctctt accgcaccct ttcaccctta cctgatcccg cttgcgcggg ccatcggcgg
      361 tttgctctct gttgcactgg tcgtgggttt cccccccagg cgttacctgg caccctgccc
      421 tatggagccc ggactttcct cccctccgcc cgtctccccc gaagaggacg acgacgaagc
      481 ggcgactgtc tggtcagctt cggcgcgcag tatagagggt ttgcgcgccc ttgtcacccc
      541 gcgttgcgca ttccaatcgc cagtgtcgcg gcgatattac gtgaagcacg gcagatattg
      601 tcataagccc cgcggaatgc ttcgtccaac gtacctatgc tggtcaatac gctgaagacc
      661 gcatcaatgc catgctgatg tacaacgcca acatcatcgg tcaggctacc cgcaatgcca
      721 atcaccggtt tatggtactt cttcgccacg tttgcgacac caatcggtac cttgccgtga
      781 atactctggc tgtcaatacg cccttcaccg gtgatcacca gcgtacaatc gtgaatatgt
      841 tcctccagat tcagcgccgt agtgacgatt tcaataccac ttttcagttc cgcaccaaga
      901 aacgccatta gcgccgcgcc cataccaccc gcagctcctg cgccggggac atctttcaca
      961 tcaacatgca gcgctttttt aatgacctcg gcatagtgag agaggttatt gtccagctca
     1021 acaatcatcg cttcactggc tcccttttgt gggccaaaga tgcgcgatgc gccgttatcg
     1081 cccaccagcg gattggtgac atcacaagcg acgcgaatga cgcaatcttt taagcgcgga
     1141 tcgaggccgg aaatatcaat atcattcaga gtattaagac taccgccgcc aaaaccaatt
     1201 tcattgccgt tggcgtcgca taatttcgcc cccagcgcct gtaccatgcc tgcgccgcca
     1261 tcatttgtag cgctgccgcc aatgccgata ataatgtttg tcgcgccgct ctccagcgcc
     1321 tgcaggatta actcgcctgt gccgcgtgaa gtggtcacga gtggatcgcg tttttccgca
     1381 ggtaccagct ccagcccact ggccgccgcc atttcaataa acgcggtttt gccatcgccg
     1441 gagatccccc aactggcatt cactttctcg cccagcggcc ctgtaaccca ggcgtgacgt
     1501 tcggaaccct gggtggctgc aatcatcgct tccaccgttc cttcgccacc gtcggcaaca
     1561 ggaacagaaa cgtactgtgc atcaggaaaa atttcccgaa atcctttttc tatcgcctgc
     1621 gcaacctcgc tggcagataa actttcttta taagagtctg gggcgattac gattttcata
     1681 cctatgcctg ttaccacatg acgccggagg gcgtttctct tattcggcct ggattccagg
     1741 cccggattgc aatacgccat ccgggcacga cgtcattaac gagtaacttc gactttcgcc
     1801 agtttttcgt agtagcacgc cagggcgcta tgatccgccg ttcctaaacc atctgcacgc
     1861 agtgcctgca tcatctccat aaccgcagct gtgagcggca gttgtgcgcc gacgccgtga
     1921 gaagtatcca gcgcattcgc cagatcctta atatgcagat caatacggaa gcccggcttg
     1981 aagttgcggt ccatcaccat cggcgctttg gcatccagca cggtactgcc cgccagtcca
     2041 ccgcgaattg cctgataaac caggtccggg ttaacgcccg ctttagttgc cagcgttaac
     2101 gcttctgaca tcgcggcaat attcagcgcc acaatgacct gatttgccag tttggtgacg
     2161 ttacctgcac cgatttcccc ggtatgcacc acggaacccg ccatcgcttt catcaaatca
     2221 tagtatttgt cgaaaatagc cttgtcgccg cctaccatca ctgacagcgt accgtcgatg
     2281 gctttcggtt caccgccgct caccggagca tccagcatat caatgccttt cgctttcagc
     2341 gcttcgctga tttcacggct tgccagcggt gcgatagaac tcatatcgat caataccgta
     2401 cctggcttcg cgccttcaat aatgccattc tcacccagcg ccacctcttt cacatgaggg
     2461 gagtttggca gcatggttat gatgacgtcg cactgttcag cgatcgcttt agccgtagaa
     2521 gctgtttctg cacctgcagc aatcacgtca gcaatagctt ctgggttacg gtcagcaacc
     2581 accagcgagt aacctgcttt cagaaggttt ttactcattg gtttacccat aatccccagg
     2641 ccaataaaac caactttcat agtcatatca atcatctctc ttgttgcggt ggtggttatt
     2701 ttttaaaggt atcagccagt ttctgagtgg cagagcggaa gacgccgaga tcgctgccga
     2761 cagccacaaa cgtcgcgccc cattccagat aacgacgcgc atcggcttcg accggcgcga
     2821 ggataccgct gggtttgccg tgcgcgctgg cacggttaaa aatgtgctga attgtttttt
     2881 gtacatccgg gtgtgatgca ttgccgagat ggcctaatgc cgcggccaga tcgctggggc
     2941 cgacgaagat gccgtctacg ccttcggtag cggcaatagc atcgacgtta tctacgccct
     3001 gctgactttc tatctggacc agaatagtga tgttcttgtt cgactgagcg aaataatccg
     3061 ccacggtgcc aaacatattg gcgcggtgag aaacggagac gccgcgaatg ccttctggcg
     3121 gataacgcgt cgatgccacc gcctgctctg cttcctcttt tgtttctaca aaaggaatca
     3181 ggaagttata gaaaccgata tccagaagac gcttaataat taccggctcg ttggtcggca
     3241 ctcgcactac tggcgcgctg gcgctgcctt tcagggccat taactgcgga ataaacgttg
     3301 aaatatcgtt tggcgcatgt tcgccatcca gcaccagcca gtcaaaccca gccaaaccaa
     3361 gaacttcagt gctaatcggg ttagagagtg ctgaccagca accaatttgt acctgtttcg
     3421 cagccagtgc ggctttgaat ttattcggga aaacatcgtt attcatcgct tatacctttg
     3481 cttatttctg caattccata cgtttaatgt cgccaactac gaagaggtag cagaccatcg
     3541 ccattagcgc tgaacatccc acgaaaacca gtgctgcatt gaaggagtgc agttcactta
     3601 ccaggtagcc aatcaccagt ggagtgacaa tggaggcaac attgccaaag acgttaaaga
     3661 cgccgccgca gaggccaaca atctctttag gcgcggtgtc agaaatcacc ggccagccca
     3721 gcgcaccaaa tcctttacca aagaaagcca gcgccatcag catgaccacc agcgtggtgt
     3781 tgttggtgta gttacataag atgatggtgg aagccagcaa cattcccagc acaatcggta
     3841 gcttacgtgc cagggtcagg gataaaccgc gtttgatcag ataatccgag aagacacctc
     3901 ccagcacgcc gcccgcaaaa ccacacagtg ctggaatcga ggcgaccaga cccactttca
     3961 gaatcgacat acctttttcc tgcaccagat aaatcgggaa ccaggtgagg aagaaccagg
     4021 tgatggtgtt gataaaatat tgtccgaaaa atacgcccaa catcatgcgg ttagagagca
     4081 attgcttgat gtaatgcagt ttgggtccgc ttgctgccgc actgcccggc tttttgtggt
     4141 ccatatcgac caccgcgcca ttttcagaga taaacttcag ctcttccgca gacatacgtg
     4201 ggtgatctgt cgggttatga atcaatttga tccacagcgc cgtcagcaca aaaccaatca
     4261 cccccataac ggtaaagacg tgctcccagc cccaggcgaa agtcagccag ccaagcagag
     4321 gcgaaaagag cgccagcgag aaatattgcg ccgagttaaa gatggcggag gcagtaccac
     4381 gttctttcgt cgggaaccag gcggcgacaa tacgggcgtt cgccgggaat gatggcgctt
     4441 ccgagaagcc gagcataaag cgcataaaga acatggagat ccctgcccag gccagcggga
     4501 acatatcaac aaagccttgc aggaaggtga atagcgacca gaaaaagagg ctgtaggtgt
     4561 aaactttttt cgagccaaac ttatcaagca gccagccgcc ggggatttgc atcagcaagt
     4621 aggcccagcc aaaagcagaa aagatgtaac ccatcgaaac cgcacttaac tgcaactctt
     4681 ttgccacttc ggtaccagca atagacagcg ttgcacgatc ggcgtagtta acggcggtaa
     4741 caataaaaat aatcagtaat attaaatagc gggtatgcac gcctttcttt ttttcgtcaa
     4801 cggtgtccag aatcatttta tttacctcgg gtacttatgc tgatttttat tattatgggg
     4861 aagatgttat ttatgagttt catttatgcc gtaacgacaa tgaactcggg aattagtata
     4921 agcagcgcga gaataataat cattgtgcaa atgctaattt aattaatact atttaaatat
     4981 tattttgagc atatgcacat aaggttgcgc gctaaagcac agatttgcgc tttaccttgc
     5041 cgggcggcat tgcaatccct gaaatgattg acattgatca catttctgcg tttaaactcc
     5101 tgacattctt atttcaccca atgaagtcat ttatttttaa atgagaccag gtcctcattt
     5161 taataacctc tggctggaga atattgcaca atggccaaca tcgaaatcag acaagaaacg
     5221 ccaactgcgt tttatataaa agttcacgac acagataatg tggcaattat tgttaatgat
     5281 aatggcctga aagcaggaac gcgttttccg gatgggctgg aattaattga acatattccc
     5341 caggggcata aagtcgcatt gctcgacatt ccggctaatg gtgaaattat tcgttatggc
     5401 gaagtgattg gttacgccgt gcgtgcaatc ccacgcggaa gctggatcga cgaatcaatg
     5461 gttgtactgc cgaaagcgcc gccgttacac acgctgccac tggcaaccaa agtcccggaa
     5521 cccttaccgc cgctggaagg atacaccttt gagggctatc gcaatgccga tggcagcgtg
     5581 ggcaccaaaa acctgctcgg catcaccacc agcgtccact gtgtggcagg cgtggtggac
     5641 tacgtagtaa aaatcattga acgcgatctg ctaccgaaat acccgaacgt cgatggcgtg
     5701 gtggggctga atcatttgta cggttgtggc gtggcgatta acgcaccggc ggcagttgta
     5761 cctatccgta ccattcacaa tatttcgctg aatcctaact ttggcggcga agtaatggtg
     5821 attggcctgg gttgtgaaaa gttgcagcct gagcgcctgc tgactggaac ggatgatgtg
     5881 caagctattc cagtagaaag cgccagcatt gtcagtttgc aggatgaaaa gcatgtcggt
     5941 tttcagtcca tggtcgagga tattttgcag gtcgccgaac gccatctaca aaaactgaat
     6001 caacgtcagc gagaaacctg tccggcttca gaactggttg tcggcatgca gtgcggtggc
     6061 agcgatgcct tttctggcgt aacggcaaac ccggcggttg ggtatgcgtc tgatctactg
     6121 gtgcgctgcg gcgcaacggt gatgttctca gaagtcacgg aagtgcgtga cgcgatccat
     6181 ctgctgacac cacgcgcagt gaacgaagag gtcggcaaac ggctgctgga ggagatggag
     6241 tggtacgata actatctcaa tatgggaaaa accgaccgca gcgccaaccc ttcgccgggc
     6301 aacaagaaag gcggtctggc aaatgtagtg gagaaagcgc tcggctccat tgctaaatcg
     6361 ggtaaaagtg caattgttga agtgctgtcg cccggtcaac gcccgactaa acgcggatta
     6421 atttacgccg cgacgccagc cagcgatttt gtctgtggca cgcaacaggt ggcttcgggt
     6481 atcaccgtgc aagtgtttac gaccggtcgt ggtacgccgt acggcctgat ggcggtaccc
     6541 gtcattaaaa tggcgacccg caccgagctg gcgaaccgct ggtttgattt aatggatatt
     6601 aacgcgggca ctatcgccac cggcgaagaa accattgaag aggtgggctg gaagttgttc
     6661 cactttattc tcgacgtcgc cagcgggaag aagaaaacct tctcggatca atggggactg
     6721 cataaccagc tggcggtgtt taacccagca ccggtgacct gatttctttt ggaacgtccc
     6781 tcgcaaaaca tggccttagt gccatgtttt tattgtttaa agcccccacg tccattaata
     6841 atgcatttgc attaccttag ttcaagctta taatttgagc agaaaacagg ctgtaaaagg
     6901 acagtgaatc atgcccgcta atgctcgctc tcacgctgta ctgaccactg aatcaaaggt
     6961 cacgatacgc ggacaaacaa ctatccccgc gccagtgcgt gaggccttaa aactgaagcc
     7021 aggccaggac agcattcatt acgaaattct gcctggtggg caagtattta tgtgccgact
     7081 gggagatgag caggaggatc atactatgaa tgcatttttg cgttttctgg atacagatat
     7141 ccagaacaac ccgcaaaaaa ctcgtccatt caacattcaa caaggaaaga aacttgtcgc
     7201 tggcatggac gtcaacattg atgatgagat tggcgacgac gaataatgga ttttccacaa
     7261 agggttaatg gttgggcgct atatgctcat ccctgttttc aggaaaccta cgacgcttta
     7321 gttgccgaag tcgagacatt aaagggaaaa gatcctgaaa attatcagag aaaagctgcc
     7381 acaaagttat tggcggtggt ccataaagtg attgaggagc atatcacggt caatccatca
     7441 tcaccggcat tccgtcatgg caagtcgtta ggctctggga aaaataaaga ctggtcacgg
     7501 gtaaaatttg gtgctggtcg ctatcgtctc ttctttcgtt acagtgaaaa agagaaagtc
     7561 atcattctgg gatggatgaa cgatgaaaac acgctgcgca cctacggtaa aaaaacagat
     7621 gcctataccg tattcagcaa aatgttaaaa agaggacatc ctcctgccga ctgggaaacc
     7681 ctcacccgag agacagaaga aacccattga tggtgttcac aatgcccgcc gaacgcattc
     7741 gtgttgacca gcgggcaccc tttctcactc cccgaccaga atcacttcaa ccccagcctt
     7801 tcgcagccct tccaggctat ccgcaggaat gccttcatca acaatgatca tgtcgatacg
     7861 ttgagtatca atgatcttat gtaaactgga acgattgaat ttactggaat cggtgaccac
     7921 gatgatccgt tccgcaactt cgcacatccg gcggtttaaa cgggcttcat cttcattatg
     7981 cgtgctgacg ccgcgctcca gatcgatcgc atctacacca agaaacagca tatcgaagtg
     8041 gtaattttgc agcgattgct cagcctgatc gccgtaaaaa gattgcgact gacggcgcaa
     8101 atgcccgccg gtcatcagca gctcaacgcc ttccgcttcc agcaacgcat tagccacgtt
     8161 cataccgttg gtcatcgcaa ttacgtcagt gtgcttgcgc atcagacgag caatctcaaa
     8221 agtggtggtc ccggaatcga ggatcacccg atgacctggc tgaatcaact caacggcagc
     8281 tttcgcaacg ctgcgtttca ttgcggtgtt cagcgcgctt ttatcttcca ctgatggctc
     8341 gactgacggc gttgtgctat cgcagatcaa cgcgccacca taggcacgca cagcgatccc
     8401 ctgcttttcc agaaacgcca gatcgttgcg gatcgtcacg gtagatacgc catacaatgc
     8461 cgacagatcg ttaacctgca cactcccttg ctgtcgcaga cgctgaatga tctgttctcg
     8521 tcgctcgctg gtgcctgtca ctcgcttctc acctgaagcg tcggtattac tcatagtaag
     8581 tcctttcgta aaactttcgt ttcatttcgt tttgcctatt aacgcctttc tattaagcaa
     8641 atgcaagccc accttgccca ttgacgcaag ctactctcgt ttcagtgact ttcattatgt
     8701 ttcttttgtg aatcagatca gaaaactatt atctttcgtt ttatttttat ctcaccatga
     8761 cgcagtatca actgaaacaa aacgaaagat taatatcgca gtaatctgaa ctggagagga
     8821 aagtgaaaca tctgacagaa atggtgagac agcacaaagc gggcaaaaca aatggaattt
     8881 atgccgtttg ttccgcacat ccgctggtgc tggaggctgc aatccgctac gccagtgcaa
     8941 accaaacgcc gttactgatt gaagcaacct ccaatcaggt agaccagttc ggcggttata
     9001 ccggaatgac gcccgccgat tttcgcggct ttgtttgtca gctcgccgac tcgttgaatt
     9061 tcccgcagga tgcgttgatt ctgggtggtg accatctggg gccaaaccgc tggcaaaacc
     9121 tgccggccgc tcaggcaatg gccaatgccg atgatttgat taaaagctac gttgcggcag
     9181 gattcaaaaa aatccacctt gattgcagca tgtcctgtca ggacgatccg attcccttaa
     9241 ctgatgacat cgtggctgaa cgcgccgccc gtctggcgaa agtggcggaa gaaacctgtc
     9301 ttgaacactt tggcgaagcc gatctggagt atgtcattgg taccgaagtg ccggtacctg
     9361 gcggcgcgca tgaaacctta agcgagctgg cggtcaccac gccggatgcc gcccgcgcca
     9421 cgctggaagc ccatcgtcac gcctttgaaa agcaaggttt gaatgccatc tggccacgca
     9481 tcattgccct ggtggttcaa cccggcgtcg aattcgatca caccaacgtt attgattatc
     9541 agcccgccaa agcgaccgcc ttaagccaga tggtcgaaag ctacgaaacg ctgattttcg
     9601 aagcgcactc taccgattat caaacgccgc aatcgctgcg ccagctggtg attgaccact
     9661 ttgccattct gaaagttggc ccggcgctga ccttcgccct gcgtgaagct ctgttctctc
     9721 tggcagcgat tgaagaagaa ctggtgccag caaaagcctg ttctggtctg cgtcaggtgc
     9781 tggaaaacgt gatgctcaac cgcccggaat actggcaaag ccactaccac ggtgacggca
     9841 acgcgcgtcg tctggcgcgt ggttatagct actcggatcg cgtgcgctat tactggccgg
     9901 acagccagat tgatgacgct ttcgctcatc tggtacgtaa tctggcggat tcaccaattc
     9961 cgctgccgct gatcagccag tatctgccgc tgcagtacgt gaaagttcgc tccggcgagc
    10021 tgcagccaac gccacgggaa ctcattatca accatattca ggacatcctg gcgcagtacc
    10081 acacagcctg tgaaggccaa taagcaaaac aaagaggaac acgctatgcc aaatattgtt
    10141 ttaagccgga ttgatgaacg cttgattcac ggtcaggtcg gcgttcagtg ggtcggattt
    10201 gcgggggcaa atctggtgct ggtcgccaac gatgaggttg ccgaagatcc ggtacaacaa
    10261 aacctgatgg aaatggtact ggcagaaggg atcgccgtac gtttctggac gctgcaaaaa
    10321 gttatcgaca acattcatcg cgccgccgat cgacagaaaa tcctgctggt ttgtaaaaca
    10381 cccgccgatt tcctgacgct ggtgaaaggt ggcgttccgg tgaatcgcat taacgttggc
    10441 aatatgcact acgccaatgg caagcaacaa atcgccaaaa cggtttctgt ggatgcgggc
    10501 gatatcgcag catttaacga cctgaaagcc gctggggtgg aatgcttcgt tcagggcatc
    10561 ccgacagagc ctgctgtgga cctctttaaa ttactttgag ggattcatca tggaaatcag
    10621 tctgttgcag gcattcgcgt tgggcattat cgcctttatc gctggcctgg atatgtttaa
    10681 cggcttaacc catatgcacc gcccagtggt cctcggcccg ttagtcgggc tggtacttgg
    10741 cgatctgcat accggtattt taaccggcgg tacgctggaa ctggtgtgga tggggctggc
    10801 cccgctggcg ggcgcacagc cgcctaatgt gattatcggt actatcgtcg gcacggcgtt
    10861 tgccattact actggcgtga aacccgatgt cgcagtcggt gtcgccgtac ctttcgctgt
    10921 cgcagtacag atggggatta ccttcctgtt ctcggtgatg tctggcgtga tgtctcgctg
    10981 cgaccggatg gcggagaacg ccgatactcg cggcattgaa cgcgtaaact atctggcact
    11041 gctggcactc ggcactttct attttctctg cgctttcctg cctatctact tcggcgcgga
    11101 acatgcaaaa accatcattg atgtcctgcc gcaacgatta atcgacggcc tcggcgtcgc
    11161 gggcggcatc atgccagcaa tcggttttgc cgtgctgctg aaaatcatga tgaaaaacgt
    11221 ctacatcccc tacttcatcc tgggctttgt tgccgcagcc tggctcaagt taccggtgct
    11281 ggctattgct gccgccgcgc tggcgatggc gctgatcgat ctgctgcgta aatctcctga
    11341 accgactcaa cctgcggcac agaaagagga atttgaagat ggcatctaat caaaccaccc
    11401 tgccgaacgt cagtgaaaac gaagaaacat tgctgactgg cgtcaatgaa aacgtgtatg
    11461 aagatcagag cattggcgcg gagctgacga aaaaagatat caatcgcgtc gcctggcgtt
    11521 ccatgctgtt acaagcgtct tttaactacg aacgtatgca ggcttccggc tggttgtacg
    11581 gtctgctgcc tgctctgaaa aagatccaca ctaataaacg cgacctggcg cgcgccatga
    11641 aggggcatat gggtttcttc aatacccatc cgtttctggt gacatttgtt atcggcatta
    11701 tccttgcgat ggagcgttct aagcaggacg ttaacagtat tcagagcacc aaaattgccg
    11761 tcggtgcgcc gctcggcggg attggcgatg caatgttctg gctaacgcta ctgccgattt
    11821 gtggcgggat tggagccagt ctggcattgc aaggctccat tcttggcgca gtcgtcttta
    11881 ttgtgctgtt caacgtggtc cacctggggc tgcgttttgg tctggcgcat tatgcttacc
    11941 gcatgggcgt ggcggcgatt ccactcatta aagctaatac caaaaaagtc ggccatgctg
    12001 cgtctatcgt tgggatgacg gtaatcggtg cgctggtggc aacctatgtt cgtttaagca
    12061 ccacgctgga aatcacagcg ggcgacgcag tggttaagtt acaggctgat gttatcgaca
    12121 aactgatgcc tgccttctta ccgttggtct acaccctgac catgttttgg ttggtacgcc
    12181 gcggctggag tccgctgcgt ctgattgcag ttaccgtggt tctcggcatc gttggtaaat
    12241 tctgccattt cctttaaata caatgaggta ttagatgtta agtattattt tgacagggca
    12301 tggcgggttt gccagcggca tggaaaaagc aatgaagcaa attctcggtg agcaatcgca
    12361 gtttatcgcc atcgattttc cggaaacctc aagcaccgcg ctgcttacat cgcagcttga
    12421 agaggccatc gcccaactgg attgtgaaga cggcattgtt tttctgacgg atttgctggg
    12481 cggcacgccg tttcgcgtag cttcaacgct ggcgatgcaa aaaccgggct gtgaagtgat
    12541 caccagcacc aatttgcaac tgctactgga gatggtgctg gagcgcgaag ggttaagcgg
    12601 tgaagaattc cgtgtacagg cgctggagtg tggacatcgt gggctgacca gtctggtgga
    12661 cgagttaggc cgctgccatg aagaatgtcc ggtcgaggaa gggatatgac acacgttctg
    12721 cgcgccagaa ggctgctgac tgaagaggga tggctcgatg accatcagtt gcgtattgct
    12781 gacggtgtca tcgcagcaat cgaaccgatt ccagtgagcg tgactgaacg cgatgcggaa
    12841 ctgctctgcc ccgcttacat cgacacccat gtacacggtg gtgcgggcgt tgatgtaatg
    12901 gatgacgcgc cggatgtact cgacaagctg gcaatgcaca aggcacgcga aggtgtcggc
    12961 agttggttgc cgactaccgt aaccgcgccg cttagtacca ttcatgcggc gctgaaacgt
    13021 attgctcaac gttgccaacg cggcggacct ggtgcgcaag tgctggggag ttatctcgaa
    13081 ggaccgtact tcacgccgca gaataaaggc gcgcatccgc cggagttgtt tcgcgagctt
    13141 gaaattgccg agctggatca attgattgcc gtttctcagc acaccttacg cgtggtagcg
    13201 ctggcaccgg aaaaagaggg ggcattgcag gccatccgcc atcttaaaca gcaaaatgta
    13261 cgagtgatgc tggggcatag cgcggcgacc tggcaacaaa ctcgcgccgc gtttgatgct
    13321 ggtgccgacg gcctggtgca ttgctataac gggatgacag gtttacatca ccgcgaaccg
    13381 ggaatggttg gcgcgggatt aacggacaag cgcgcctggc tggaactgat agccgatggt
    13441 catcatgtgc atccggcggc aatgtcgctg tgttgttgct gtgcgaaaga gagaatcgta
    13501 ctgatcaccg acgcgatgca ggcagccggg atgccggatg gtcgctatac gttatgtggc
    13561 gaagaagtgc agatgcacgg tggcgttgtc cgtaccgcgt ccggtgggct ggcgggcagt
    13621 acgctgtctg ttgatgcggc agtgcgcaac atggtcgagt tgacgggcgt aacgcctgcg
    13681 gaagccattc atatggcgtc gctgcatccg gcgcgaatgc tgggtgttga tggtgttctg
    13741 ggatcgctta aaccgggcaa acgcgccagc gtcgttgcgc tggatagcgg gctgcatgtg
    13801 caacaaatct ggattcaggg tcaattagct tcgttttgat ggtttgctcc attattgggc
    13861 cttcacttcc cccgtaaggc ctttcttttt ctttcgtttt gatctgtgca gcggtgtcgg
    13921 atgcgacgct aacgcgtctt atccgaccta cagttggtga ccgcaaggcc ggataaagcg
    13981 tttgcgccgc atccagcaat cccttttgct tcctttatct tttctttcaa cgatcacgga
    14041 tttcgtttta tttccttttt ctccattgaa ctttcggttt cttttctata gattttaatc
    14101 aacgaaagac atcaccaagt gaaatgaaac gaaaggcaag tgaaagcgac aacgcccgac
    14161 gtcaagttca tcagactaag gattgagtta tgccagaaaa ttacacccct gctgccgccg
    14221 caaccggtac atggactgaa gaagagatcc gccatcagcc tcgcgcatgg atccgttcgc
    14281 tcaccaacat cgacgcgcta cattccgcgc ttaataactt ccttgaaccg ttactgcgca
    14341 aagagaatct gcggatcatc ctgaccggag ccggaacctc ggcatttatc ggtgacatca
    14401 tcgcgccgtg gctcgccagc cataccggta aaaacttcag cgccgtaccg accaccgatc
    14461 tggtcactaa tccgatggac tacctgaacc cagcccatcc gctgctgttg atctccttcg
    14521 gtcgatccgg caacagcccg gaaagcgtcg cagccgtgga actggcaaat caatttgtac
    14581 cggaatgcta tcacctgccg atcacctgca acgaagcggg cgctctttac caaaacgcga
    14641 tcaacagcga taacgcgttt gccgtgctga tgcccgcaga aacgcacgat cgcggctttg
    14701 cgatgaccag cagcattacc accatgatgg ccagctgcct cgcggttttc gcacctgaga
    14761 cgatcaacag ccaaaccttc cgcgacgtgg cggatcgttg ccaggcgatc ctgacctcac
    14821 tgggcgattt cagcgaaggt gtgtttggtt acgcaccgtg gaaacggatc gtttatctcg
    14881 gcagcggtgg cttacagggc gcagcacgcg agtcggcgct gaaagtgctg gaactgacgg
    14941 cgggtaaact ggcggccttt tatgattctc caaccggatt ccgtcatgga ccaaaatcgc
    15001 tggtcgataa cgaaacactg gtggtggtat ttgtctccag ccacccttac acccgtcagt
    15061 atgatcttga tctgctggct gaacttcacc gtgacaacca ggcaatgcgt gtaatcgcca
    15121 tcgccgcgga aagcagcgac atcgtcgctg ccggtccaca tatcatcctg ccaccgtcac
    15181 gtcactttat cgacgttgag caggcatttt gcttcctgat gtacgcccag acgtttgcac
    15241 tgatgcagtc gctgcacatg ggcaatacgc cggatacccc atcagccagt ggcaccgtta
    15301 accgcgtggt gcaaggcgta atcattcatc cgtggcaggc ataagaggat cgcattatga
    15361 gcattatctc caccaaatat ctgttacagg acgcccaggc caatggctac gcggtgcctg
    15421 cttttaacat tcataacgcc gagacgatcc aggcgatcct cgaagtgtgc agtgaaatgc
    15481 gatcgccggt gatcctcgcc ggaacgccgg ggacctttaa acatatcgcg ctggaagaga
    15541 tctacgccct gtgcagcgcc tattccacaa cctacaacat gccactggcg ctgcatctcg
    15601 accaccacga atcgctggat gatattcgcc gtaaagtcca cgcaggtgtg cgcagtgcga
    15661 tgatcgacgg cagccacttc ccgtttgccg agaacgtaaa gctggtgaaa tcggttgtcg
    15721 acttctgcca ctcccaggat tgcagcgtgg aagcagaact gggccgcctg ggcggtgttg
    15781 aagatgacat gagcgttgac gccgaaagtg cattcctgac cgatccacaa gaagctaaac
    15841 gctttgtcga actgactggc gtcgacagct tggcggtagc gattggtacg gcgcacggct
    15901 tatacagcaa aacgccgaag attgatttcc agcggctggc ggaaattcgt gaagtggtgg
    15961 atgttcctct ggtgctacat ggtgccagcg atgttccgga tgaatttgtc cgtcgcacta
    16021 ttgaacttgg cgtcacaaaa gtgaacgttg ccacagaatt aaaaatagcc ttcgccggcg
    16081 cggttaaagc ctggtttgcg gaaaatccgc agggtaatga tcctcgttat tatatgcgcg
    16141 tcggaatgga tgcgatgaaa gaagttgtca gaaataaaat taatgtctgt ggttcagcga
    16201 atcgaatttc agcataatca ttagtttttt atgatttatc ccaatgtact tcccgattaa
    16261 tatcggggag tgccttaatg gaaaaggaga tgactaaacc ttaataaata gatcagtaca
    16321 atatcgcaac aataatatat ttaaaaaaat tatattattc aactttatgg tgaggattac
    16381 acaatgagca gtccaaatat tctcttaacc cgtattgata accgtctggt tcacggtcag
    16441 gttggcgtga cctggacatc caccatcggt gcgaatctgc tggtagtcgt ggatgatgtt
    16501 gtcgctaacg atgatattca acagaaatta atgggtatta ccgcagaaac ctacggcttt
    16561 ggcattcgtt tctttactat cgaaaaaacc attaacgtca tcggcaaagc tgcaccgcat
    16621 cagaagatct tcctgatttg ccgtacgcca caaacggtac gtaaattggt agaaggtggc
    16681 attgacctga aagatgtcaa cgtcggcaat atgcatttct cggaagggaa aaagcaaatc
    16741 agcagtaaag tttatgtcga tgaccaggat ctcacggact tacgttttat taaacaacgt
    16801 ggcgtgaatg ttttcattca ggacgtccct ggcgatcaaa aagaacaaat ccctgactaa
    16861 atctaaaatc gccttaatat tggtttgagg taataaaaat gcatgaaata accctacttt
    16921 agggattatc cctggcggcg ttagttttcg ttctggggat tgatttttgg ctggaagcct
    16981 tatttttatt ccgcccgata atcgtttgta ccctaactgg cgctattctc ggtgatattc
    17041 agactggctt aattaccggt ggtctgacag agttggcttt cgccggatta acccctgcag
    17101 gtggtgttca gccgcccaac ccgattatgg cgggtctgat gaccaccgtc attgcatggt
    17161 ctacgggcgt tgatgccaaa acggcaattg gtcttggcct gccgtttagt ttgttaatgc
    17221 agtacgtcat tctgttcttc tattctgctt tctcattatt tatgaccaaa gccgataaat
    17281 gcgcgaaaga ggcggatacg gcagcgtttt cccgacttaa ctggacaacg atgctcatcg
    17341 tcgcttcagc gtatgcggtg attgctttcc tctgtactta cctggcacaa ggggcgatgc
    17401 aggcgctggt gaaagcgatg cccgcttggc tgacccacgg ctttgaagtg gctggcggta
    17461 ttctgcctgc cgttggtttt ggcttgctgc tgcgcgtgat gttcaaagca caatatatcc
    17521 cttacctgat cgccggcttc ctgtttgttt gctacatcca ggtcagcaac ctgttgccgg
    17581 ttgccgtact gggcgcaggc tttgcggtgt atgagttttt caatgcgaaa tcccggcagc
    17641 aagcgcaacc gcagcccgtt gccagtaaaa atgaagaaga ggactacagc aatgggatct
    17701 gaaatcagta aaaaagatat cacccgtctg ggctttcgtt cgtcgctgct gcaagcgagc
    17761 tttaactacg aaaggatgca agcgggcggt tttacctggg cgatgttgcc gatcctgaaa
    17821 aagatttata aggatgacaa accgggccta agcgcagcga tgaaagataa cctcgaattt
    17881 attaataccc acccgaatct ggtcggattc ctgatggggt tattaatttc gatggaagaa
    17941 aaaggagaaa accgcgacac cattaaaggc ctcaaagtgg cactgtttgg cccaatcgcc
    18001 gggattggcg atgcgatttt ctggtttact ttgttgccga ttatggcggg aatttgctca
    18061 tcatttgcca gccagggaaa cctgctgggg ccgattctat ttttcgccgt ttacctgctt
    18121 atctttttcc tgcgcgtcgg ctggacccac gtcggttatt cagtcggcgt gaaggcgatc
    18181 gataaagtgc gagagaactc gcagatgatt gctcgctcgg caaccatcct cgggatcacg
    18241 gtaatcggcg ggctgatcgc ttcgtatgtg catattaacg tggtgacatc gtttgccatc
    18301 gacagtaccc acagcgtcgc gctgcaacag gatttcttcg ataaagtctt cccgaacatt
    18361 ttaccgatgg cctacaccct gctgatgtat tacttcctgc gggtgaaaaa agcgcatccg
    18421 gtgctgttaa tcggcgtgac ttttgtgctc tctattgttt gttccgcatt cggcattttg
    18481 taaatggaac gaggcactgc gtctggcggt gcctctttac ttaaggaatt tcatcctgtg
    18541 caaacccttc agcaagttga aaactatacg gcgttaagtg aacgtgccag cgaatattta
    18601 ttggccgtga tccgtagcaa accggatgcc gtgatttgcc tggcgaccgg agccacgcca
    18661 ttactgacgt atcattatct ggtagaaaaa atccactagc agcaggttga tgtcagccag
    18721 ctcaccttcg tgaagctcga cgaatgggtg gatctgccat taacgatgcc tggtacctgc
    18781 gaaactttcc tgcaacagca tatcgtgcag ccgctggggc tacgtgaaga ccagctcatc
    18841 agctttcgct ccgaagagat aaatgagaca gagtgcgaac gggtaacgaa cctgattgcg
    18901 cgcaaaggcg gtctggattt atgcgttctc ggattgggga aaaacggtca tcttgggctg
    18961 aacgaaccgg gagaaaacct gcaaccggcc tgccatatca gtcaacttga tgccagaaca
    19021 cagcaacatg agatgttaaa aaccgcgggt cgccccgtga ctcgtgggat caccttaggc
    19081 ctgaaggata ttctcaatgc ccgcgaggtt ttgttactgg tgactggcga aggaaagcag
    19141 gatgcgacag aacgttttct cacggctaaa gtctctacct ctatcccggc ttcgtttttg
    19201 tggctgcaca gtaactttat ttgtttaatt aatacctaac cagatgaaaa atttgctcaa
    19261 aatgtttaaa ttaacttatg caacagtcac gcattatatt aaataacatt tgactgggtt
    19321 gaacataacg ccaatagcaa aaggagttat gttcaacaat ttaattttag atatacatat
    19381 aacttgttct attaataaac catctcgtat tctgactcgc cccctggcgc agcaaagtca
    19441 tgcacttata ctc
//
LOCUS       DABSYT020000084        19285 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.084, whole genome shotgun sequence.
ACCESSION   DABSYT020000084 DABSYT020000000
VERSION     DABSYT020000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.084"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(281..1930)
                     /gene="pgi"
                     /locus_tag="ID725_RS17735"
                     /old_locus_tag="pgaptmp_003547"
     CDS             complement(281..1930)
                     /gene="pgi"
                     /locus_tag="ID725_RS17735"
                     /old_locus_tag="pgaptmp_003547"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGV7852060.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2167..2363
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2455..3804
                     /gene="lysC"
                     /locus_tag="ID725_RS17740"
                     /old_locus_tag="pgaptmp_003548"
     CDS             2455..3804
                     /gene="lysC"
                     /locus_tag="ID725_RS17740"
                     /old_locus_tag="pgaptmp_003548"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGV7852061.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4057..4329
                     /locus_tag="ID725_RS17745"
                     /old_locus_tag="pgaptmp_003549"
     CDS             4057..4329
                     /locus_tag="ID725_RS17745"
                     /old_locus_tag="pgaptmp_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGV7852062.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4330..5202)
                     /gene="rluF"
                     /locus_tag="ID725_RS17750"
                     /old_locus_tag="pgaptmp_003550"
     CDS             complement(4330..5202)
                     /gene="rluF"
                     /locus_tag="ID725_RS17750"
                     /old_locus_tag="pgaptmp_003550"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGV7852063.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5550..6497
                     /locus_tag="ID725_RS17755"
                     /old_locus_tag="pgaptmp_003551"
     CDS             5550..6497
                     /locus_tag="ID725_RS17755"
                     /old_locus_tag="pgaptmp_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGV7852064.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6567..7373
                     /locus_tag="ID725_RS17760"
                     /old_locus_tag="pgaptmp_003552"
     CDS             6567..7373
                     /locus_tag="ID725_RS17760"
                     /old_locus_tag="pgaptmp_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGV7852065.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7383..7790
                     /locus_tag="ID725_RS17765"
                     /old_locus_tag="pgaptmp_003553"
     CDS             7383..7790
                     /locus_tag="ID725_RS17765"
                     /old_locus_tag="pgaptmp_003553"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HGV7852066.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7790..8284
                     /locus_tag="ID725_RS17770"
                     /old_locus_tag="pgaptmp_003554"
     CDS             7790..8284
                     /locus_tag="ID725_RS17770"
                     /old_locus_tag="pgaptmp_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGV7852067.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            8350..9147
                     /locus_tag="ID725_RS17775"
                     /old_locus_tag="pgaptmp_003555"
     CDS             8350..9147
                     /locus_tag="ID725_RS17775"
                     /old_locus_tag="pgaptmp_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGV7852068.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            9158..9982
                     /locus_tag="ID725_RS17780"
                     /old_locus_tag="pgaptmp_003556"
     CDS             9158..9982
                     /locus_tag="ID725_RS17780"
                     /old_locus_tag="pgaptmp_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGV7852069.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10034..11263
                     /gene="sorE"
                     /locus_tag="ID725_RS17785"
                     /old_locus_tag="pgaptmp_003557"
     CDS             10034..11263
                     /gene="sorE"
                     /locus_tag="ID725_RS17785"
                     /old_locus_tag="pgaptmp_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HGV7852070.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11646..12335
                     /gene="pepE"
                     /locus_tag="ID725_RS17790"
                     /old_locus_tag="pgaptmp_003558"
     CDS             11646..12335
                     /gene="pepE"
                     /locus_tag="ID725_RS17790"
                     /old_locus_tag="pgaptmp_003558"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGV7852071.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12426..14057)
                     /locus_tag="ID725_RS17795"
                     /old_locus_tag="pgaptmp_003559"
     CDS             complement(12426..14057)
                     /locus_tag="ID725_RS17795"
                     /old_locus_tag="pgaptmp_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGV7852072.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14277..17960)
                     /gene="metH"
                     /locus_tag="ID725_RS17800"
                     /old_locus_tag="pgaptmp_003560"
     CDS             complement(14277..17960)
                     /gene="metH"
                     /locus_tag="ID725_RS17800"
                     /old_locus_tag="pgaptmp_003560"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGV7852073.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18160..18984
                     /gene="iclR"
                     /locus_tag="ID725_RS17805"
                     /old_locus_tag="pgaptmp_003561"
     CDS             18160..18984
                     /gene="iclR"
                     /locus_tag="ID725_RS17805"
                     /old_locus_tag="pgaptmp_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGV7852074.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4563 a         5003 c         5050 g         4669 t
ORIGIN      
        1 acgattaaaa atctatttta ggattaacct cacggtatga tttccgttaa atcacagaca
       61 aggggattta tctgataaaa caatgccgac ttatagtcgg cattgttttt aaggttattt
      121 aattaatcgt gctatgcatt aattgccgaa tacgatgtac atatcggcat cgacctgtag
      181 gcctgataag acgcgtaaag cgtcgcatca ggcaccagtt gccggatgcg gcgtaaacgc
      241 cttatccggc ctacatagcg gcatcaggca tgaacgatga ttaaccgcgc cacgctttat
      301 agcggttaat cagaccattg gtcgagctat cgtggctgct gatttcttta tcatctttca
      361 gctctggcag aatacggttc gccagctgtt tacccagttc cacgccccac tggtcgaagg
      421 tgaagatgtt caggatcacg ccctgagtaa agattttgtg ctcatacagc gcaatcaacg
      481 cacccaggct gaacggagtg atttcgcgca acaggataga gttggtcggg cggttacctt
      541 cgaacacttt gaacggcacc acgtagtcaa gcgttgccgg atctttaccc tgatcgcgat
      601 attcctgctc aaccacttcg cgggatttac caaacgccag cgcttcggtc tgggcgaaga
      661 agttagacag cagtttctgg tggtgatcag agagcgggtt atgggtgata gccggagcga
      721 tgaaatcgca cggtaccatt ttggttccct ggtggatcag ctggtagaac gcgtgctgac
      781 cgttagtgcc tggttcaccc cagataatcg ggccagtctg gtaatccaca acgttaccgt
      841 tacggtcaac atacttaccg ttggactcca tattgccctg ctggaagtac gccgcgaaac
      901 ggtgcatata ctggtcatac ggcagaatcg cttcagtttc cgcaccaaag aaattgttgt
      961 accagatgcc aatcagcgcc aacagtacag gcaggttttt ctcggcaggc gtggtggaga
     1021 aatgcttgtc catcgcgtgt gcgccagaaa gcagttcaac gaagttatca aagccgatgg
     1081 agagaacaat cgacaggcca atcgctgacc acaaagagta acgaccgccg acccagtccc
     1141 agaactcgaa catgttggca gtatcaatac caaactcgcc aacggctttg gcattggttg
     1201 agagcgccgc aaagtgtttc gcaacgtgtt tttcatcacc tgccgctttc aggaaccagt
     1261 cacgcgcgct atgggcgttg gtcatggttt cctgagtggt gaaggtttta gatgctacca
     1321 ggaacagcgt ggtttccggg tttacttttt tcagcacttc agcgatgtga gtcccatcga
     1381 cgttagaaac aaagtgcatg ttcaggtgat ttttgtacgg acgcagtgct tcggtcacca
     1441 tgtacgggcc gaggtcagaa ccgccgatcc cgatgttcac tacgtcagtg attgctttgc
     1501 cggtataacc cttccactca ccggagataa tcgcttccga gaaggttttc atcttctcca
     1561 gcaccgcgtt gacttctggc ataacatctt tgccatcaac cagaatcggg gtattgctac
     1621 gattacgcag cgctacgtgc agcacggcgc ggttttcagt gcggttgatc ttctcgccag
     1681 agaacatcga cttaatcgcg cccgccagat cgcactcttt cgccagatct tgtaatttcg
     1741 ccagcgtctc ttcagtgatg cggtttttgg agtaatccac cagcatctga tcgtcgaagg
     1801 ttgcggagaa cttagaaaaa cgatcgccgt ctttagcaaa aagatcggcg atcgtaacgt
     1861 ctttcatttc atcgaagtgt ttctgtagtg cctgccaggc agcggtctgc gttggattga
     1921 tgtttttcat tagcaatact cttctgattt tgagaattgt gactttggaa gattgtagcg
     1981 ccagtcacag aaaaatgtga tggttttagt gccgttagcg taatgttgag tgtaaaccct
     2041 tagcgcagtg aagcatttat tagctgaact actgaccgcc aggagtggat gaaaaatccg
     2101 catgaccccg tcgttgacaa ccgccccgct caccctttat ttataaatgt actacctgcg
     2161 ctagcgcagg ccagaagagg cgcgttgccc aagtaacggt gttggaggag ccagtcctgt
     2221 gataacacct gagggggtgc atcgccgagg tgattgaacg gctggccacg ttcatcatcg
     2281 gctacagggg ctgaatcccc tgggttgtca ccagaagcgt tcgcagtcgg gcgtttcgca
     2341 agtggtggag cacttctggg tgaaaatagt agcgaagtat cgctctgcgc ccacccgtct
     2401 tccgctcttc ccttgtgcca aggctgaaaa tggatcccct gacacgaggt agttatgtct
     2461 gaaattgttg tctccaaatt tggcggtacc agcgtagctg attttgacgc catgaaccgc
     2521 agcgctgata ttgtgctttc tgatgccaac gtgcgtttag ttgtcctctc ggcttctgct
     2581 ggtatcacta atctgctggt cgctttagcg gaaggactgg aacctggcga gcgattcgaa
     2641 aaactcgacg ctattcgcaa catccagttt gccattctgg aacgtctgcg ttacccgaac
     2701 gttatccgtg aagagattga acgtctgctg gagaacatta ctgttctggc agaagcggcg
     2761 gcgctggcaa cgtctccggc gctgacagat gaactggtca gccacggcga gctgatgtcg
     2821 accctgctgt ttgtcgagat cctgcgcgaa cgcgatgttc aggcacagtg gtttgatgta
     2881 cgtaaagtga tgcgtaccaa cgaccgattt ggtcgtgcag agccagatgt agccgcgctg
     2941 gcggaactgg ccgcgctgca gctgctccca cgtctcaatg aaggcttagt gatcacccag
     3001 ggatttatcg gtagcgaaaa taaaggtcgt acaacgacgc ttggccgtgg aggcagcgat
     3061 tatacggcag ccttgctggc ggaggcttta cacgcatctc gtgttgatat ctggaccgac
     3121 gtcccgggca tctacaccac cgatccacgc gtagtttccg cagcaaaacg cattgatgaa
     3181 atcgcgtttg ccgaagcggc agagatggca acttttggtg caaaagtact gcatccggca
     3241 acgttgctac ccgcagtacg cagcgatatc ccagtctttg tcggctccag caaagaccca
     3301 cgcgcaggtg gtacgctggt gtgcaataaa actgaaaatc cgccgctgtt ccgcgcgctg
     3361 gcgcttcgtc gcaatcagac tctgctcact ttgcacagcc tgaatatgct gcattctcgc
     3421 ggtttcctcg cggaagtttt cggcatcctc gcgcggcata atatttcggt agacttaatc
     3481 accacgtcag aagtgagcgt ggcattaacc cttgatacca ccggttcaac ctccactggc
     3541 gatacgttgc tgacgcaatc tctgctgatg gagctttccg cactgtgccg ggtggaggtg
     3601 gaagaaggtc tggcgctggt cgcgttgatt ggcaatgacc tgtcaaaagc ctgcggcgtt
     3661 ggcaaagagg tattcggcgt actggaaccg ttcaacattc gcatgatttg ttacggcgca
     3721 tccagccata acctgtgctt cctggtgccc ggcgaagatg ccgagcaggt ggtgcaaaaa
     3781 ctgcatagta atttgtttga gtaaatactg tatggcctgg aagctatatt tcgggccgta
     3841 ttgattttct tgtcactatg ctcatcaata aacgagcctg tactctgtta accagcgtct
     3901 ttatcggaga ataattgcct ttaatttttt tatctgcatc tctaattaat tatcgaaaga
     3961 gataaatagt taagagaagg caaaatgaat attatcagtt ctgctcgcaa acgaattccc
     4021 gtacactgaa ctctctggct cactgagggc accatcatgg cactcccccg tattacccaa
     4081 aaagagatga ctgaacgcga gcagcgtgaa cttaaaacac tgctggatcg ggcgcggatt
     4141 gcgcatggtc gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat
     4201 aaattaatgg tcgagcgtga ggctgaagcg aaaaaagccc gccagttaaa gaaaaagcag
     4261 gcttataaac cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc
     4321 aggcgctgat tagcgcccct tcttttttcg ccccggcgag gtaaaacgct taccgttgga
     4381 cgccgggcga ccgccttttt ccgccgtttt ttccatctta acgactggac gtttgatgcc
     4441 cgctgttttc ggtttggcct tcgctttagg ttttgcctcg gaagaggaat tttcgatgag
     4501 cttaaagagg tcgattaact catcgtcggt taaatcgcgc cactccccca gcggaatgcc
     4561 gcttaagctg acgttcataa tgcgcgtacg ttccagcttt ttcacttcat agccgaaatg
     4621 ctcgcacatg cgccggatct gacggttcag cccctgcacc agggtaatgc ggaagacaaa
     4681 cggcgcttct tttttgactt tgcacttttt agtcaccgtc ccgaggatcg gcacccccgc
     4741 ccccatacca cgaataaatt catcggtaat cggtttatcg accgtcacca gatactcttt
     4801 ctcatgatcg ttacccgcac ggaggatctt attcaccaga tcgccgtgat tggtgaggaa
     4861 aatcagcccc tgggagtctt tatccaggcg gccaatcggg aacacgcgtt tgctgtggtt
     4921 aacgaaatcg acaatgttat cgcgctcgcc atcttcggtg gtgctgacaa tgcctacggg
     4981 cttgttcagg gcgataagta ccaaatcttc ggcttcccga ggctcaatca actgaccgtt
     5041 tactttcaca acgtcgccgg gtttcacctg atcgccaatg gtggctcgct tgccattaag
     5101 gaacacattg ccttgctcga tatagcgatc cgcttcgcgg cgtgagcaaa ttccgctttc
     5161 gctgatgtat ttatttaaac ggactgatga gtcgggcagc ataaattctc ctgtaaaacg
     5221 cggaatatac cgtacctctt agcggggaaa aacagggtta tgcggcaacg tcgaaattaa
     5281 atgtgatctg ccacgccttt tatcacaaaa gtgtctccgg aagtacaatg caaatttgcg
     5341 tggcaaaata aggataatca acatattacg aatcgctgca cacctgtgct gataactact
     5401 gcgcagagaa acaaatgtgc aagagaaagc gctatttctc cccatgcaaa gagtgaaaaa
     5461 tcgctttgaa aggccatcat gatggggtta tatcagatca agcggtgaga aagcgtattt
     5521 gcacaaatgt gcagaaccag gaggcggcaa tggaaaacag tgacgatatc cgtttgattg
     5581 tgaagattgc ccaactctat tacgaacagg atatgacgca ggcgcaaatc gcgcgcgaac
     5641 tgggtattta ccgcaccaac atcagccgct tgcttaaacg aggccgcgat cagggaattg
     5701 tcaccatcgc catcaactat gactacaacg aaaatctctg gctggagcag caactgaagc
     5761 aaaagtttgg cctgaaagac gttgtggtgg tgtcgggaaa tgatgaggat gaagagactc
     5821 aactggcgat gatggggtta cacggcgcgc aactgctgga tcgcttgctg gagcctggcg
     5881 atattgtcgg tttttcctgg ggtcgcgcgg tgagcgcact ggttgaaaac ttgccgcagg
     5941 cggggcaatc gcggcagtta atctgcgtac caattattgg cggaccgtcc ggtaaactcg
     6001 aaagccgcta tcacgtaaac acattaacct acagcgcggc agcgaagctg aaaggggaat
     6061 cgcatctcgc ggattttccg gctctactgg ataacccatt aattcgtaat gggatcatgc
     6121 agtctcagca ctttaaaacc atctctgcct actgggataa tctggatgtc gccctggtgg
     6181 gaattggctc accggccatt cgcgacggcg ctaactggca tgcgttttat ggtggtgaag
     6241 agagtgacga cctgaatgcc cgccaggttg ctggcgatat ttgctcgcgc ttttttgata
     6301 ttcacggcga aatggttgaa acgaatatga gcgaaaaaac actctctatc gaaatgaata
     6361 aattaaagca ggcacgatat tccattggca ttgccatgag tgaagaaaaa tacagcggaa
     6421 ttattggtgc actgcgtgga aaatatatta attgtctggt aacgaatagc agcacagctg
     6481 aactattact gaaataaaaa tcttcccgat gtttatcgta ataaaaatta cggcgggtcc
     6541 ctattatcta aaatcaggag tctgttatgc aaacgtggtt aaatttgcag gataaaatca
     6601 ttatcgtcac cggcggcgca tccggtattg gtctggcgat tgtagaggaa ttattagcac
     6661 aaggcgcaaa cgtacagatg gtagatattc acggtggcga tggacaatat gaaggtcata
     6721 aaggctatca gttttggccg accgatattt ccagcgccaa agaggtaaat catacggtag
     6781 cggaaattat ccagcgtttt ggtcgcatcg acggtctggt caataacgcc ggggtcaatt
     6841 tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca gtatgaactc aacgaagctg
     6901 cattcgaaaa aatggtcaat atcaaccaga aaggcgtttt tctgatgtcg caggcggtgg
     6961 cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa tgtttcctca gaaagtgggc
     7021 tggaaggctc agaaggccaa agctgttacg ccgctaccaa agccgcgctc aatagcttca
     7081 cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg tgtggtcggt atcgcgccgg
     7141 ggattctgga aaaaaccggg ctacgcacgc cggaatatga agaggcgctg gcgtggacgc
     7201 gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa aaacgccatt cctattgggc
     7261 gcgccggaag attagcagaa atcgctgatt ttgtttgtta tctgctgtct gaacgcgcca
     7321 gctatatcac cggagtaacc actaacattg cgggcggcaa aacgcgcggc taaggaggct
     7381 ttatggtcaa cgccattttt tgtgcccacg gcaaattagc ttgcgccatg ctggaatcgg
     7441 ttcagatggt ttacggcaac gccaatgttg aagccgttga atttgtgccc ggtgaaaacg
     7501 ccggagacat cgtagcaaag ctggaaaaat tagtaagcat tcacaatcaa aatgagtggc
     7561 tgattgccgt ggatttacag tgcggcagcc cgtggaatgc ggcagcgatg cttgcgatgc
     7621 gcaatcctcg tctgcgggta atcagcggac tttctctgcc gctggcgctc gaactggttg
     7681 ataaccagga cagcatgaat gttgatgaac tgtgtgaaca cctgacgcaa atcgcaaagc
     7741 aaacctgcgt tgtctggaaa caactggcaa cgacagagga ggatttctga tgaacattac
     7801 gctcgcccgc attgatgacc gcctgatcca cggtcaggtc actaccgtct ggtcgaaagt
     7861 ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt tataacgatg aagttcgccg
     7921 gacattattg cgtcaggctg cgccaccggg aatgaaggtt aacgtggtca atattgaaaa
     7981 agccgtcgcg gtttatcaca acccgcaata tcaggacgaa accgtttttt atcttttcac
     8041 tcgcccacag gatgccttag cgatggttcg ccagggggta aaaataggca ccctgaatat
     8101 tggcggcatg gcctggcggc ccggtaaaaa acagttaacc aaagcggttt cattagatga
     8161 tgatgatatt aacgcatttc atgagttaaa taatctaggc gtcatactgg atttacgcgt
     8221 cgtagcgtca gatccctcaa tcaatattat ctacaaaata aacgaacagt taatcgcaaa
     8281 ttaaataata gcgcctgtgt acatatgctt taacaggcag ggatgacgac acttaaaagg
     8341 tgacaaatca tggaaataag taccctacaa atcattgcta tatttctttt ttcctgtatt
     8401 gccggaatgg gcagcgtgct ggatgaattt cagactcatc gcccgttaat tgcctgtacg
     8461 gtgattggtt taattctcgg tgatttaaaa accggaataa tactcggtgg tacgctggaa
     8521 ttgatagctc tcggctggat gaacgtcggc gcggcgcaat ctccggattc tgcactcgcc
     8581 agcataatct ccgccattct ggttatcgtt ggtcagcaaa gcatcgccac cggaatcgcc
     8641 atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg tgtttgcccg taccatcacc
     8701 gtagtgttcc agcacgcggc ggataaagca gcggaagaag cacggtttcg cactctcgat
     8761 attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg tcgccattcc ggcactgatt
     8821 gtctcgctgt tcgtcagcgc cgatatggtg agcaatatgt taaacgccat tcccgaattt
     8881 gtgacccgag gtctgcaaat tgctggcggt tttatcgtgg tagtcggtta cgccatggtg
     8941 ctacgtatga tgggcgtgaa atatttgatg cctttctttt tcctcggttt cctcgcaggt
     9001 ggctacctcg atctcagtct gctggcgttc ggtggcgtcg gcgtgatcat ggccctggtc
     9061 tacatccagc taaatccaca gtggcgtaaa gctgaacctc aactcctggc ccccgcttcc
     9121 accgcccttg accaacttga cgattaacgg agccatcatg gaacagagaa aaattacacg
     9181 cggcgatctg gtgagcatgt ttctgcgctc caacctgcaa caggcgtcct ttaactttga
     9241 acgtattcac gggctgggtt tttgctacga catgatcccc gccatcaagc gactttaccc
     9301 agtaaaagag gatcaggttg cggcgctcag gcgacacctg gtgttcttta ataccacgcc
     9361 agccgtatgt ggcccggtca tcggcgtcac tgccgccatg gaagaggcgc gggccaacgg
     9421 cgcggaaatt gatgacggca ccattaacgg catcaaagtc ggtctgatgg gaccattggc
     9481 aggggttggc gatccactgg tctggggaac gctgcgcccg attaccgccg cgctcggcgc
     9541 atcgctggcg ctgtctggca acattctcgg cccgctgctg ttcttcttta ttttcaacgc
     9601 ggtgcgtctg gcgatgaagt ggtatggcct acagctcggc tttcgcaaag gggtgaatat
     9661 cgtcagcgat atgggcggga atgtgctgca aaaactcacc gaaggcgcgt cgattctcgg
     9721 actgtttgtg atgggcgtgc tggtcaccaa atggacgtca atcaacgtac cgttggtggt
     9781 ttcacaaacg catgccgccg atggctccac cgtcaccatg accgtgcaga acattctcga
     9841 tcaactttgc cctggtttgc tggcgctcgg tctgacgcta ctgatggttc gtctgctcaa
     9901 caaaaaaatt aacccggtat ggctgatttt cgccctgttt ggcttaggga ttatcggcaa
     9961 tgcgctgggc ttcctgtcct gattcttcgc cccggcacga ctgccggggc catcgttcaa
    10021 catgaggtgg tttatgaaaa caacagctct gcgtctttat ggtaaacgtg atttacgcct
    10081 ggaaaccttt gaccttcctg aaatgcagga ggatgaaatc ctcgcgacgg tggtcactga
    10141 cagcctgtgc ctctcttcct ggaaagaggc caatctgggt gaaaaccata aaaaagtacc
    10201 cgacgatgtg gcgaccaacc caatcatcat cggccacgag ttttgcggcg atattctggc
    10261 cgtgggtaaa aagtggcagc acaaattcca gccgggtcag cgttatgtga ttcaggccaa
    10321 cctgcaactc cccgaccgcc cggactgccc cggctactcc ttcccgtggg taggcggcga
    10381 ggccacgcat gtggttattc ccaacgaggt catggaacaa gattgcctgc tggcatacga
    10441 cggcgaaacc tattttgaag gctcgctggt tgaaccgctt tcctgcgtga ttggcgcgtt
    10501 caacgccaac tatcatcttc aggaaggtag ttataaccac acgatgggga ttcgcccgca
    10561 agggcgcatg ctgatcctcg gcggcaccgg accaatggga ctgttggcga ttgattatgc
    10621 gctacatgga cccgttaacc cgtcgctgct cgtcattacc gataccgaca acgataaatt
    10681 gagttatgcg cgcaagcact atccatcaga accgcaaaca ctgattcatt atctcaatgc
    10741 cgccgatgca gcatttgata cgctaatggc gctgagtggc ggtcacggct tcgatgatat
    10801 tttcgtcttt gtgcctaatg aaggactggt gactctcgcc tcttccttgc tggcgacaga
    10861 tggttgcctg aatttcttcg ccggaccgca ggataaacat ttcagcgcgc caattaattt
    10921 ctacgatgtg cattatgcat ttacccacta cgtgggcacg tcaggcggca ataccgacga
    10981 catgcgcgca gcggtcaaat tgattgaaga gaaaaaagtg caggccgcaa aagtggtaac
    11041 acatattctt gggctgaatg ccgcgggcga aaccacgctt gaattgcctg ccgtcggcgg
    11101 cggcaaaaag ctggtgtata ccgggaaata cctgccgctg acgtcactca cgcagattca
    11161 ggatcaagca ctggcggcga ttctggcgcg tcatcagggg atctggtccg gtgaggcgga
    11221 gcaatatctg ctcactcatg cagaggcaat ttcccatgat taatcgcgct acttaactct
    11281 gcatgtcgct ggctggtcac cgcggcaact tcgcgacctg atgtgcggct gattgctgaa
    11341 gcggcccaat gtgcacgaga aggataaaaa aacatcagcg ccttaagggg cgctcaaatt
    11401 ggtggcaaag tacacattgt ttatgccgga tgcggaataa agccttattc ggcctacata
    11461 atctcgaaaa ttcaatatat tgcagttatc tggtaggcct ggtaagcgta gcgcatcagg
    11521 caatgctgtg tttgtcatca gtctcagcgc ccggtgcaat acgtgacatc gtgagccatg
    11581 tcagtaagtg gttaaattcg cagtggtagc atgccttccg tgatttctta ctaaccggag
    11641 aagtaatgga actgctttta ttgagtaact cgacgctgcc gggtaaagcc tggctggaac
    11701 atgcactgcc gctgattgct gaacagttgc agggtcgccg ctcagcggtg tttatccctt
    11761 tcgctggcgt aacgcagacc tgggatgatt acacagcgaa aacggctgcg gttctcgctc
    11821 cgctgggtgt ttctgtcacc ggtattcata gcgttgtcga tcccgttgcc gcgattgaaa
    11881 atgcggaaat tgtgattgtc ggcggcggta atactttcca gttgctgaaa cagtgccgcg
    11941 agcgcgggct gctggctcca attactgacg tggttaaacg tggcgcgctg tatattggct
    12001 ggagcgcagg cgctaacctt gcttgcccaa ccattcgtac caccaacgat atgccgattg
    12061 tcgatccgca aggtttcgat gcgctaaatc tgttcccgtt gcaaatcaac ccgcacttca
    12121 ccaacgcgct gccggaaggc cataaaggtg aaacccgtga gcagcgtatt cgcgaactgc
    12181 tggtcgtcgc gccagaactg acgattattg gtctaccgga aggtaactgg atcacagtga
    12241 gtaaaggtca cgcaacgctg ggtggcccga ataccaccta tgtgtttaag gccggtgaag
    12301 aagcggttcc gctggaagct ggtcaccgtt tttaagtgaa ttgccggatg catgatccgg
    12361 catcttgttc atagtgcctg atgcgacgct tgacgcgtct tatcaggcct acaggattca
    12421 caatattaat actcgtcccg tccctcatct tcatcaggct gttccagcac actgtaagcc
    12481 accgaacaaa acagcgagtt cagacgctgc atatctccca gtaagcctaa atgtagcgaa
    12541 ctggtttcaa tgctttgcac gttttgctga tgcaggcgat cgacgtgggc gtgggaatag
    12601 cggcgattaa gaatgcgaaa gcgatgcttg ctgcggcgca aacgacgagc gctggtgaca
    12661 tcgccagaga agaacaccga cattgccagc tttaaattac tgagcaattg ctcatagagc
    12721 gcatccagtt ccttcaagcc atcaagcgaa aatgctcgcc ttgcagccag cgatttgtcg
    12781 gcaatttcac tactcatgcg ctcgacgata tcggaggcct gttcaaggtt gagcgacatt
    12841 tcgatgatct ccgcccagcg gcgcgactct tcttctgcca gttcctcttt tggcatccgc
    12901 gccagataca gcttaatggc ggtatagaga acgttgatat catctgccag cttacgcagc
    12961 tctttctcct gccttggctc gccgtgcatc actttattca gcccttccat catctgttcc
    13021 atggcgtcgc caatgcgcag ggtttcgcgc gcggcgttcg ccagagcaag cgtgggcgta
    13081 tccagcgcgc tgacatccag atgtttaggc cgtagctggg tatccagttc cggttcatcg
    13141 cgaataatcg ttttgcaaaa ccgtgccatc gggtcaacaa atggcagcat ggccaggcaa
    13201 cgtacaaggt tgtagaagac gtggaaatag atcaccagtt ccgcttttgg caatgacaac
    13261 ttccccattg tctctgccag caaatggaca aacggcagga taatcaggct acccaccagc
    13321 ttaaacagca gactacccag cgcgacacgg cgggctgcgg cattggcggc actgttgttg
    13381 agcatcgcca gcaggccgga accgaggtta gcaccaatca ccagacagag cgccacgggg
    13441 aaggagataa tgcctgcggc ggtcagagtc gcggtcagca gtacagcagc aaggctggag
    13501 tagctgataa tggcgaacat cgcgccaatc agcgcatcca gcagaatatc gccggtcagc
    13561 gaggcaaaga tcacctgaac gccgtttgcc tgggtgatcg gcgttacggc ctgcacaatc
    13621 aactccagcg ccagcaaaat cagcccaaga ccaataccga cgcggcccag ttgcccggcg
    13681 cgtgactgtt tgcgtccgag gaaaaaaatc acgccgataa aaataagtaa cggtgacagc
    13741 caggataagt cgaaggtgag aatacgcgcc attagcgccg tcccgacatc tgcccccagc
    13801 acaatgacca gagccggtgc gagggctacc agatcctgag cgacgaacga ggtcaccagc
    13861 atggtggtgg cattactgct ctgtaccagt gcggtaacgc cgatccccgc gcaaaaggcg
    13921 agcggctttt tttcgacgct ccggctaagg acagtacgca aacgcgcgcc gaagacgcgc
    13981 attacgccgg ttcgaacaat atgagtcccc cagaccagca gggcgacggc agaaagcagg
    14041 ttaagcagcg ttaacacgag ttcaggttct ccttatcgtt atatgttcct gaagcaattg
    14101 aatgaccaaa ggaccggaaa cacgctgact ggcatcttta ctgtgccttt tgacattcgt
    14161 ttttccggct aagattattc cagcctgttt ccagtataag tgtttcatat taataaagag
    14221 acagggcact cattgagcgc cctgtttgtt gtaaggaaaa gtgacagatt tgtgaatcag
    14281 tccgcgtcat accccagatt cggtgccagc cagcgctcaa catcggagac gctcatacct
    14341 ttacggcggg catagtcttc aacctgatcg cgctgaattt gcgccaccgc gtagtacttg
    14401 ctgtccgggt ggctgaaata ccaacccgaa accgatgcac cgggccacat ggcgaaagat
    14461 tctgtgagtt tcatgccggt atgtttttcc acttccagca gctcccagat ggtggctttt
    14521 tccgtatgtt ccgggcaggc cgggtagccc ggtgccggac ggatgccctg gtagttttcg
    14581 cggatcagtt cttcgttgct gaggttctcg ttcggcgcat agccccagta gactttacgc
    14641 acacgctcat ggagatactc cgcaaaggct tcggctaaac ggtcggcaag cgctttcacc
    14701 atgattttgt tgtaatcatc gtgctgcgct tcaaaggcat cagccagtgc gtcctcttcc
    14761 agcccgccag tcacggcaaa tgcgccgatg taatctgctt taccagaaag cttcggcgca
    14821 acgaagtcag cgagacagta gttagcgaat cccgtttttt cggtctgttg acgcagatgg
    14881 tggctgacgt tgatcacatg ggtgcgcgtt tcgtcacggt agatttcaat gtcatcgccc
    14941 acacggtttg ccgggaacag gcccaccacg ccacgcggat tcagcgtttt ctcggcgctt
    15001 aatttatcca gcatgtcgtt ggcgtcttta aacagccgct gcgcctcaac gcccaccact
    15061 tcatcttcca gaatgcgcgg atacttcccg gccagcgacc aggtcataaa gaacggtgtc
    15121 cagtcgatgt aattacgcag agtttcgatg ctggcttcaa cttcctgcac gccgagacgg
    15181 tgcgccaccg gcggcgtgta agcctgccag tcaaaagcga agtcgttatc gcgcgccgct
    15241 tccagcgtga ccggtggtgt gcgtggtttc ttgcgcccgt gctgaatacg tacggtttcg
    15301 tactccttgc gggtacgagc gacaaaatca tcgcgctgag tatcggaaag cagcgccgcc
    15361 accacaccaa cggtgcgcga ggcattctgc acatacaccg tcgggccgct gtagttctgc
    15421 tcgattttca ccgccgtgtg cgcttttgag gtcgtcgcgc caccgatcag taacggaatg
    15481 gtgaagccct gacgctccat ctctttcgcc acgttaacca tctcgtccag cgacggcgtg
    15541 ataagccccg aaagtccaat cagatcagca ttcacttctt tagcggtacg gagaattttt
    15601 tccgcaggca ccataacgcc gagatcgaca atttcgtagt tgttacattg cagcaccaca
    15661 ccaacgatat ttttaccgat gtcgtggacg tcgcccttca cggtggcgat caccatcttg
    15721 ccgttggttt tgccctgctc tttgctggct tcaataaacg gttcgaggta ggccactgcc
    15781 tgcttcatca cacgcgccga tttgaccacc tgtggcagga acattttccc ttcgccgaac
    15841 agatcgccga ccacgttcat gccgtccatt agcgggcctt caatcacttc aatcgggcgc
    15901 gtagcctgct ggcgggcttc ttcggtatcc tgctcgataa actcagtaat gcctttgacc
    15961 agcgagtatt ccagacgttt attcacttcc cacgagcgcc actccgcctg ctgggcgtta
    16021 gcggtgtcgt cggttttgct gccgcgatat ttctcggcaa gctccagtaa acgctcagtg
    16081 ccatcatcgc gacgattaag aatcacatct tccaccgcgt cgcgcagttc agcgggcagg
    16141 tcgtcgtaaa tcgccagttg cccggcgttg acgatcccca tatccatgcc attgcgaata
    16201 gcgtagtaga ggaacaccgc gtgaatggct tcgcgcaccg gatcgttgcc acggaatgag
    16261 aaagaaacgt tagatacgcc gccggaaatc agcgcgtgcg gcagttcgcg tttgatgtct
    16321 tcgcaggcgc cgataaagtc ctgcgcatag ttgttgtgct cttcaatgcc ggtggcgacc
    16381 gcgaagatgt tcgggtcgaa gatgatatct tctggcggga agccaacctc ttcggtgagg
    16441 attttgtacg cccgacggca aatctcaatt ttccgtgcgc gggtatcggc ctgcccctgt
    16501 tcgtcaaagg ccattaccac caccgctgca ccgtaacgac gcaacagttt cgcatgatgg
    16561 ataaaggcgt cgacgccctc tttcattgag atagagttaa caatgccttt gccctgaata
    16621 cacttcagcc ccttttcgat gacctcccat tttgaggagt cgatcataat cggcacgcga
    16681 gcgatatccg gttcaccggc aatcagattg agaaaacgca ccatcgccgc ttcggcatcg
    16741 agcatccctt catccatgtt gatatcgata atctgcgcgc cgttttccac ctgctgacgc
    16801 gctacatcca gcgcctcgct gtatttctct tctttgatca ggcgcttgaa cttagcggaa
    16861 ccggtgacgt tggtgcgttc acccacgttc acaaacaggc tatcgtcgcc aatgttcagc
    16921 ggctccaggc cggacaaacg gcaggctacg ggaatttcag gcagtttgcg cggcgctaat
    16981 ccttctactg cacgactcat cgctgcaata tgttgtggcg tggtgccaca gcagccgccg
    17041 acgatattga gaaaacccgc ttgcgcccat tcacgtatct gttttgccat cgtgtcggcg
    17101 tcgagatcgt actcaccaaa ggcgttgggt agcccggcgt tcgggtgcgc ggtgacgtag
    17161 cattccgcaa tccgtgatag ttcctgcacg tactggcgca gttcatctgg ccccagcgcg
    17221 cagttgaggc caaaagtcag ggcttcggca tggcgcaggg agttgtaaaa tgcttcggtg
    17281 gtctgtccgg agagcgtgcg cccggaggcg tcggtgatgg tgccggagat cataatcggc
    17341 agctcaacgc ctaacgcttc gaactccgtt ttcaccgcaa agaccgccgc tttggcgtta
    17401 agggtgtcga aaacggtttc aatcagaatc agatccgcac cgccttccac taatgctttg
    17461 gtggattcgc gataggccgc caccagctgg tcaaaagtga tgttgcggaa tgccggatcg
    17521 ttcacgtccg gagaaatgga cgccgtgcgg ttggttgggc cgagaacgcc ggcaacgtag
    17581 cgcggtttct ccggcgtgcg cgcggtccac tcgtcagcac aggcgcgcgc cagtttcgcc
    17641 gccgcaaagt tgatttccgc cgacagggat tccatctggt aatccgccat cgcaatggtc
    17701 gtggagttga aggtgttggt ttcgatgata tccgcgcccg cttcaaagta ggcgttgtgg
    17761 atagcggcga tcacttccgg tttgcttagc accagcagat cgttattgcc tttgaggtcg
    17821 catggccagt cggcaaagcg ttcaccacga aaatcggctt cgttcagtcg atagctctgg
    17881 atcatggtgc ccataccgcc gtccagcacc agaatacgtt catttaactg cgcacgcagt
    17941 tgttccactt tgctgctcac acttgctccc gacacgctca acccagattt aataaaaatt
    18001 caacaaacca tactggcata aacgcatctg tggtaaaagc gaccaccacg caacatgaga
    18061 tttgttcaac attaactcat cggatcagtt cagcaactat tgcattagct aacaataaaa
    18121 atgaaaatga tttccacgat acagaaaaag gagactgtca tggtcgcacc cattcccgcg
    18181 aaacgcggca gaaaacccgc cgttgccacc gcaccagcga ctggacaggt tcagtcttta
    18241 acgcgtggcc tgaaattact ggagtggatt gccgaatcca atggcagtgt ggcactcacg
    18301 gagctggcgc aacaagccgg gttacccaat tccacgaccc accgcctgct aaccacgatg
    18361 caacagcagg gtttcgtgcg tcaggttggc gaactgggac actgggcaat cggcgcacat
    18421 gcctttatgg tcggcagcag ctttctccag agccgtaatt tgttagcaat tgttcaccct
    18481 atcctgcgca atctaatgga agagtctggc gaaacggtca atatggcggt gcttgatcaa
    18541 agcgatcacg aagcgattat tatcgaccag gtacagtgta cgcatctgat gcgaatgtcc
    18601 gcgcctatcg gcggtaaatt gccgatgcac gcttccggtg cgggtaaagc ctttttagcc
    18661 caactgagcg aagaacaggt gacgaagctg ctgcaccgca aagggttaca tgcctatacc
    18721 cacgcaacgc tggtgtctcc tgtgcattta aaagaagatc tcgcccaaac gcgcaaacgg
    18781 ggttattcat ttgacgatga ggaacatgcg ctggggctac gttgccttgc agcgtgtatt
    18841 ttcgatgagc accgtgaacc gtttgccgca atttctattt ccggaccgat ttcacgtatt
    18901 accgatgacc gcgtgaccga gtttggcgcg atggtgatta aagcggcgaa ggaagtgacg
    18961 ctggcgtacg gtggaatgcg ctgacttttt ctggcgggca gaggcaatat tctgcccgtc
    19021 atacctgagt ggtaatagaa taagggtgtc tgttaatcgc attgacgtca aaataactta
    19081 ttgtcataca cttcatacac ttttattaac tcagcattat ttttaaacat caaaccactt
    19141 aattattaac aaaatcgcaa gatctcattc caccgaaact tttacggctg cattatgaga
    19201 ttgagcgatt tatgatattt ctttagttgt aatcatatta aattttttca ctgttctgat
    19261 agttaaaatt caagacatca ataaa
//
LOCUS       DABSYT020000085        18613 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.085, whole genome shotgun sequence.
ACCESSION   DABSYT020000085 DABSYT020000000
VERSION     DABSYT020000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.085"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..773
                     /locus_tag="ID725_RS17810"
                     /old_locus_tag="pgaptmp_003562"
     CDS             <1..773
                     /locus_tag="ID725_RS17810"
                     /old_locus_tag="pgaptmp_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7852075.1"
                     /translation="GWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLIN
                     QLASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGF
                     TASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIV
                     ALCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLF
                     ASDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            799..1593
                     /gene="yfaU"
                     /locus_tag="ID725_RS17815"
                     /old_locus_tag="pgaptmp_003563"
     CDS             799..1593
                     /gene="yfaU"
                     /locus_tag="ID725_RS17815"
                     /old_locus_tag="pgaptmp_003563"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HGV7852076.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ID725_RS17820"
                     /old_locus_tag="pgaptmp_003564"
     CDS             complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ID725_RS17820"
                     /old_locus_tag="pgaptmp_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGV7852077.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ID725_RS17825"
                     /old_locus_tag="pgaptmp_003565"
     CDS             complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ID725_RS17825"
                     /old_locus_tag="pgaptmp_003565"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGV7852078.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ID725_RS17830"
                     /old_locus_tag="pgaptmp_003566"
     CDS             complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ID725_RS17830"
                     /old_locus_tag="pgaptmp_003566"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGV7852079.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPTKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ID725_RS17835"
                     /old_locus_tag="pgaptmp_003567"
     CDS             complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ID725_RS17835"
                     /old_locus_tag="pgaptmp_003567"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGV7852080.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7090..8448
                     /gene="glpT"
                     /locus_tag="ID725_RS17840"
                     /old_locus_tag="pgaptmp_003568"
     CDS             7090..8448
                     /gene="glpT"
                     /locus_tag="ID725_RS17840"
                     /old_locus_tag="pgaptmp_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGV7852081.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            8453..9529
                     /gene="glpQ"
                     /locus_tag="ID725_RS17845"
                     /old_locus_tag="pgaptmp_003569"
     CDS             8453..9529
                     /gene="glpQ"
                     /locus_tag="ID725_RS17845"
                     /old_locus_tag="pgaptmp_003569"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGV7852082.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(9753..10823)
                     /locus_tag="ID725_RS17850"
                     /old_locus_tag="pgaptmp_003570"
     CDS             complement(9753..10823)
                     /locus_tag="ID725_RS17850"
                     /old_locus_tag="pgaptmp_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852083.1"
                     /translation="MPNTSIHLSRCNILQNNKLQPTEIYKESQQTAKLEFFCDEFLKI
                     SQSRYGLSTSTDSIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKN
                     EFAADYEPYTLTFTRGENNEGILSVESKEGFISQRTINLNEYETAINIINEHVIKENI
                     HNTVQSPTEKYISKINSSDKPYKTSSKESIKSQLYSDQRNIQTCYYILRKNTEWYKYA
                     SSEERYDKFKNSSKEIKNTYKQIVQAQKKLNQMKYINKLSGELIDITDKKLAPLINDS
                     FSYTRDFFAYSKQEKNIFTFDNSKFVDPKEKEGLMVQHSNGQLVITGKYCPEGVQTAF
                     TQEQYDKLIRYINIFFTFPKLE"
     gene            complement(11287..12165)
                     /locus_tag="ID725_RS17855"
                     /old_locus_tag="pgaptmp_003571"
     CDS             complement(11287..12165)
                     /locus_tag="ID725_RS17855"
                     /old_locus_tag="pgaptmp_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7852084.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
     gene            12317..13507
                     /locus_tag="ID725_RS17860"
                     /old_locus_tag="pgaptmp_003572"
     CDS             12317..13507
                     /locus_tag="ID725_RS17860"
                     /old_locus_tag="pgaptmp_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7852085.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            13588..14238
                     /gene="inaA"
                     /locus_tag="ID725_RS17865"
                     /old_locus_tag="pgaptmp_003573"
     CDS             13588..14238
                     /gene="inaA"
                     /locus_tag="ID725_RS17865"
                     /old_locus_tag="pgaptmp_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGV7852086.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(14292..14546)
                     /gene="yfaE"
                     /locus_tag="ID725_RS17870"
                     /old_locus_tag="pgaptmp_003574"
     CDS             complement(14292..14546)
                     /gene="yfaE"
                     /locus_tag="ID725_RS17870"
                     /old_locus_tag="pgaptmp_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGV7852087.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(14546..15676)
                     /gene="nrdB"
                     /locus_tag="ID725_RS17875"
                     /old_locus_tag="pgaptmp_003575"
     CDS             complement(14546..15676)
                     /gene="nrdB"
                     /locus_tag="ID725_RS17875"
                     /old_locus_tag="pgaptmp_003575"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGV7852088.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(15903..18188)
                     /gene="nrdA"
                     /locus_tag="ID725_RS17880"
                     /old_locus_tag="pgaptmp_003576"
     CDS             complement(15903..18188)
                     /gene="nrdA"
                     /locus_tag="ID725_RS17880"
                     /old_locus_tag="pgaptmp_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGV7852089.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKHFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            18316..18459
                     /gene="ypaB"
                     /locus_tag="ID725_RS17885"
                     /old_locus_tag="pgaptmp_003577"
     CDS             18316..18459
                     /gene="ypaB"
                     /locus_tag="ID725_RS17885"
                     /old_locus_tag="pgaptmp_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGV7852090.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
BASE COUNT         4380 a         4394 c         4887 g         4952 t
ORIGIN      
        1 ccggctggtt ctggatgttt gtgattgaag gattgttggc agtcggcgct ggggtattca
       61 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa ctagaaaaaa
      121 cgttgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg
      181 atgcgctgcg taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg
      241 cggtttacgg attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag
      301 tgggctttac agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
      361 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
      421 tactggcggc gggcattggt attggtctgt ccgggctgct ttctccagta ctggcgatcg
      481 tagcgctgtg tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc
      541 cgacacaact gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgcttg
      601 gtgcagtggg cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca
      661 gcgatgcggc gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt
      721 tcactctgcg tgtaaatcgc accgttgcgc agaccgacgt ggcacatcat taaataggtt
      781 aaggaacacg atgaacgcat tattaaccat ccctttaaag aacgtttacg caagggcgaa
      841 gtgcaaattg gtctgtggtt aagctcaacg actgcctata tggcagaaat tgccgctact
      901 tccggttatg actggttgct gattgacggg gagcatgcgc caaacaccat tcaggatctt
      961 tatcaccagc tacaggcggt agccccctat gccagccaac ccgtgatccg tccggtggaa
     1021 ggcagtaaac cgctgattaa acaagtcctg gatattggcg cgcaaactct actgatcccg
     1081 atggtcgata ctgccgatca ggcacgtcag gtggtgtctg ccacgcgcta tcctccctac
     1141 ggtgaacgtg gtgttggggc cagtgtagca cgggctgcgc gctggggacg cattgagaat
     1201 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     1261 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacct
     1321 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     1381 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     1441 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     1501 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     1561 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     1621 gggactgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     1681 cgagcaccag gtcacgatca aagccctgtt ccagcatgcg caaggcaatt ttaatagctt
     1741 gttcatgcat gccttcctgc aaaccttcta atttaccttc ctgccaacca atttgatgtc
     1801 cttcctgccg tagccgttca gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt
     1861 cggcaatctc ctgaataaaa cgggtgaaac gggaggtatc gccgcattgc agcagataat
     1921 taaacagtgt ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc
     1981 tgtcgaccat accgattaaa tcgcggtcgc gaatatgctt ttgaatcagt tcaagcagag
     2041 ctatacgccg atgttgcatg atctcatcgt caggtacgat ggtaatatcc accaacggaa
     2101 aagcttcggt gtacaactgc cgggcaagtt gcggatcgtc aaactcatcc agccagttga
     2161 gtgagtacgg gtagggcgag gtttcgccat gataaaacag caacggcacc accagcggga
     2221 ctctgtcata gcctttgtcc aggtgacgct gcatggcggc agtggcatag cgcattagcc
     2281 gaaaagccat attcttttct gcagagcttt gatgttcaat cacgcagtag atatagccgt
     2341 caccgtcgct ggtttccacg gaccacaaaa catccgagta gtaagcgcgt aaactttttt
     2401 caataaaact agtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg cgcagtggtt
     2461 ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg tgtgaacata aaggttttaa
     2521 atactgcatc atgcggggag gaggttgttg attcggtcat ggttgtccgt cacctgatat
     2581 ttatgatgac gcgacattac ggttatccca ggcctttgac atctgccagt ttcagccttt
     2641 gcgagacgtt ttcagaggaa ttttcactgc gttgcaacgc atacattggg cacggaatcg
     2701 tgtacgcaaa aatgcccggt aaccacatgc ttaccgggca tcagaaagga gtttaagcca
     2761 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag acttgtggac atctcaatct
     2821 gccatttaca ggtttcgcag tcggtgacca ccagatctgc gccgctttct tctatctggc
     2881 ggaacagtgg tgcgccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     2941 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggatgttac
     3001 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     3061 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     3121 acagccagcg ggtcgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     3181 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     3241 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     3301 ctttatcggt aaagccgttg gcgatcagcg gtacgccgca gcatttttct ttgctgagca
     3361 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     3421 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct
     3481 gagccgccac gctgcgatac cagcgacgaa acgtgccgaa ggagtatttc ggtagcgtgc
     3541 ggcgatgatc gatttttaac gccgcatcaa gcaactgccg caccggtttc agcgaggtag
     3601 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatggctta
     3661 aaacaaaatt gcgcagcgac gggcgcgtgg tgtcatattt cgcccgtgcg cgctggatga
     3721 tatcgccaat cttcacatcg gacgggcagg cgacttcgca acgtttgcag ttgatgcaat
     3781 atttcagtgc ctcgtcatac agtgcgccat cttttaaacg cagacgctcg ccatccggcc
     3841 cggcttgttt tggccctgga taaccgggat tcacccggct cactgggcag gcggtggtgc
     3901 agacggtgca cttaatgcag ttttcgaagc tggtgtcatt cattgttggc ctcctgcgcg
     3961 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     4021 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gtaaacaggt tttcgatggt
     4081 ttgccctgcc tgcgacgggc gtagcgtctg atcagtggtt acaccgaact gctgccacgg
     4141 ttgcggcgca aaaaaatctc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     4201 aaggccgaga atcggctctc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     4261 actgccgctg gcgagaaccg cgaaacgtgg acgcagcggg atatcggcgt gattgcgggt
     4321 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     4381 ccacacgcca ccctgacgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     4441 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     4501 acgccacagt ttgtcatcag ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     4561 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     4621 acgggcgata ttcaccgcgc ggaactcggt ggcgttattg cgcaggacat ccagttccgg
     4681 cagctctatt tctgcggttt caacggcaag gtcgagttca cgcaacgaag ctgccgcaag
     4741 gtgcgcctga aaatccatca gtccgctaat tcccactaca catattttct tcgtgggcag
     4801 cggccagacg gggacttctg gcgaacttag ccaggtagag cgcagagtgc cgagcggcgt
     4861 aatccgctgg tgaggcagtt ctacgctgcc ctgcaattgc gcaccgcttt cagcgattaa
     4921 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggc ccgagaagag agtaaggatg
     4981 tgcaggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca cagcttgacc
     5041 atctggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     5101 acgcgtgaca atggcacagc gcaggccgtg tttttgcagc tgcaggccac agagtaatcc
     5161 ggcgaggccg ccgcccataa tgatagtatc aaagcgcatc tttctgctcc ttctccagac
     5221 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgca tctccccagg
     5281 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     5341 gcgcggacgt cgtgacgtta aaacgttgca gcagtccagc agcgcggcag gcgcagagtt
     5401 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     5461 cgtttaaatt ttctaccgcg tactgcactt cacccgcagt taccgcttcg cactcacata
     5521 ccaggctacg gtgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     5581 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     5641 cgggttcctg tgaacccgga agtgccagat cggcggtcgt acaggggcgc gtgttgccca
     5701 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     5761 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     5821 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     5881 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     5941 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     6001 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     6061 cggaaggttt acggcagcgg ttgatcacat gctgattaat gcggtgatcc atgatcagca
     6121 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     6181 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     6241 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     6301 catgagcggt aaggataaca gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     6361 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     6421 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     6481 cttcgcaggc gcgaataaaa gtggcctgga aggagaggtc atcttccggc agggtgataa
     6541 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     6601 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     6661 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     6721 gcagcccgcg cagggcacag tcgcgggcaa ttccggctcc cgttgcgccg ccgccaatga
     6781 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     6841 tttaccattt agccatagta aaaacatgag ttgtttgatt tcgcgcatat tcgctcataa
     6901 ttcgaaagtg agacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
     6961 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     7021 aaacggcagg ttctctcact gaatcaggct gttaatcata aataagacca cgggccacgg
     7081 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgccgcg
     7141 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     7201 tatgctgctt actatttggt tcgtaagaac tttgcgctcg ctatgcctta cctggtggag
     7261 cagggattct cacgcggtga tttaggtttt gcgctttctg ggatctcgat cgcctatgga
     7321 ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc
     7381 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     7441 tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg
     7501 tggccgccat gcggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     7561 gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct gctgttcctg
     7621 ctggggatgg cctggttcaa tgactggcac gcggcgctct atatgcccgc tttctgcgcc
     7681 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg
     7741 ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag
     7801 gagctgaccg cgaagcaaat cttcatgcag tacgtgctgc cgaacaaact gttgtggtat
     7861 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
     7921 acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcttc
     7981 tatgagtatg cgggtattcc gggcaccctg ctgtgcggct ggatgtcgga taaagtcttc
     8041 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
     8101 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
     8161 ggcttcctga tctacggccc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
     8221 aaaaaagcgg caggaacggc agcgggcttt accggtctgt ttggttacct gggcggttcg
     8281 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
     8341 atggtaatga ttggcggcag cattctagcg gttatcttgt tgattgttgt gatgattggc
     8401 gaaaaacgtc gccatcagga attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
     8461 gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
     8521 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
     8581 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
     8641 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attacctcga
     8701 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
     8761 cgcgatagat ttcacgctgg atgaaattaa gtcgttgaaa tttaccgaag gtttcgatat
     8821 tgaaaacggt aaaaaagtgc agacttatcc agggcgtttc ccaatgggta agtccgactt
     8881 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
     8941 gaaaaatatc ggtatctatc cagaaatcaa agcgccgtgg ttccatcatc aggaagggaa
     9001 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaagacga
     9061 taaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
     9121 acccaaaatg ggcatggatc tcaatctggt acagctgatt gcctataccg actggaatga
     9181 aacgcagcag aaacagccgg acggaagctg ggttaattac aactacgact ggatgtttaa
     9241 gccgggtgcc atgaaacagg tggcggaata tgcagatggt attggtccgg attaccatat
     9301 gttgattgag gagacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
     9361 tcagcagaac aagctggtag tgcatcctta taccgtgcgg tcagataaac tgcctgaata
     9421 cacaactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg taaatgggtt
     9481 gtttactgat ttccctgata aagcggttaa attccttaat aaagagtaat gctgctactg
     9541 caggatggcg ttcatgcgcc atcctctgct aaagcgaaat aatgctacta ccggttattc
     9601 ttttcattaa ccggtgcgtc ctgtaaaaaa gatgccaaca taattttcgt aaaactcatt
     9661 cgttaaaaaa gctaacactg ggcattgtta ttctttaaac agtatgaata ggtctcaata
     9721 ataaattgta atgcagttta tactgtaaag tattattcta atttcggaaa ggtaaagaaa
     9781 atattaatgt atcttattaa tttatcatat tgttcttggg tgaatgctgt ttgcacacct
     9841 tcagggcaat atttaccagt aataaccagt tgcccattgc tgtgttgtac cattaaacct
     9901 tctttttcct ttggatctac aaacttagaa ttatcaaatg tgaagatatt tttttcctgt
     9961 tttgagtaag caaagaaatc tcgtgtatag ctgaaactat catttattaa tggtgctaac
    10021 tttttgtcgg tgatatcgat aagctcacca cttaatttat tgatgtattt catttggttc
    10081 aattttttct gcgcttgtac aatttgcttg taggtatttt taatttcctt agaagaattc
    10141 ttgaatttat catagcgttc ctcggaggat gcgtatttgt accattctgt attttttctc
    10201 agaatgtagt agcaggtctg tatatttctt tgatcgctgt aaagttgact tttaatactt
    10261 tccttactgc tggttttata aggtttatca gagctgttta tttttgaaat gtatttttct
    10321 gtcggtgact ggaccgtgtt gtggatattt tcttttataa catgttcatt gatgatgtta
    10381 attgcagttt catattcatt taagtttatt gttctttgtg aaataaaccc ttctttgctt
    10441 tcgacactca aaataccttc gttgttttca cctcgggtaa aggtaagggt atagggttca
    10501 taatccgctg caaattcatt tttatacgtt atgacatgtt cgacaaaatg tcttattggt
    10561 acgaaaccat aactgtgaac gtcaattttt tgggtcttga tactatcaat tgcatctgag
    10621 gctttcgtga aaaaagccaa taaattagct atgctgtctg ttgaggttga taacccgtat
    10681 ctactttgag atatttttag aaactcatcg caaaaaaact caagtttggc ggtttgttgt
    10741 gattctttgt aaatttctgt tggttgcaat ttattatttt gtaaaatatt acagcgagat
    10801 aagtgaatag atgtatttgg cataaatgat tccttgtatt gtgagttaac aagatacaat
    10861 ttaatctttg cagaatatat tcactatcaa atttagctca ttttattacg aagaccttta
    10921 cgcatgttag aaagtatcat tattgttaat aaatacggta ataagtaaca aacagatatt
    10981 gataacttcc ctgacaagtt attaggtatc ccacttgaag attgagaatg gctccagagt
    11041 aaactgtgta ctgttcgccg gagtgtcatt actctcaaca atcggttttg acagtgagtc
    11101 gggcgcttca taccggtttc tacctggagt cattgtcact tctcccaact ggataaatat
    11161 atcgccaatt cacaaagtgg gccaaaatca ggtgaaaacg aattattacg cctggtaaag
    11221 gctatagtac aactaagtgt ggcgaatcag gcagtcttgc atttgtcatc aatctcatgt
    11281 cttacatcaa atttttccac tcaccactag ttgttccgta cacatttgta gtaaaaacag
    11341 tggactgcct tctaccggct tttctttagc cagcaacgaa atctgtcgat agaaggttgg
    11401 ttcgagagga acggaacaca attcatctgc aatggttttc agcgttaact ctggtaataa
    11461 ggcgatgcct aatccctgtc gaataaaact gatcgctgta tccgggtgat taaattcata
    11521 ttttatctgt ggggtaatat tcttttcttt aaatagtgcc ataatgctta attcatagcg
    11581 tcctttacta atgattaatg gttcacccat taattcttcc actgtaatag tgctgttttt
    11641 tgccagagca tggttgggcg gtaacactac ggtgaattta tcctgataaa tcggcacgga
    11701 gtacattccg ctcaccggaa aatgaacaaa accggcatca atggattcat tctgaagcga
    11761 atcaataatc gctgtgctat tttcctcaaa aggaataatt tttatattcg gatgatgaat
    11821 ttcaaagtag cgaatcacaa ccggtaagat acaggcgcag acgctgggga aacaaccaag
    11881 tctcagcgtt ctttgagggt tctgcttttc ctgctcggct atctctttta ctgcgttaac
    11941 ttcccgatta atggcgcgca gatgttgcac aatgcggtga cctgctgtgg tcagttgtat
    12001 agctttacgg cgatcgcgta acagtatatt cacaccaagt tcttgttcga gggcagcaat
    12061 tgcctgactt accgccgact gcgtcatgaa caatcgcttg ctggcttcag taaagccacc
    12121 gtaatccagc acggcgagta aagaatttat ttgggtaaga gtcattagag tttgctaatc
    12181 aataatatta aaacgattaa tttctctaat agcatgccgc catttatact tctgttctgc
    12241 aaagtacatc acaatttgac gcaaccgcag taacgggagt gttgtctgcg tgcgtcatcc
    12301 tgacaggcgg tagagtatga aagagaagtt atggacgaag gatttttggg caataaccat
    12361 catcagcttt attattttct tcgtctttta tgttttacta acattgttgc caatttatat
    12421 ctctgaccgc ttgcatgcct ctcctgataa agcaggtttg ttggtgactt tatttttaat
    12481 tgcggcgatt gttattcgac cctttgccgg gcaatgggtg ggtaaatatt cgaataaaac
    12541 tattctggtg ctctcttctc tggccttttt ggtggtcact gcgctgtatc ctttttgcca
    12601 ctcaatagaa tcactgcttt ttattagggt gcttcatggt attaccttcg gggttatcac
    12661 aacggtaaag ggaacgattt ccgcgcggct gatcccggcc tcccgacgtg gggagggcat
    12721 cagttttttc tctctggcaa tggggctggc aatggtggtc gggccgtgga ttggcctgaa
    12781 tatggcgcgc tgggaggcct ttaatatggc tttctggtta tgtactggcg ttgcggcggt
    12841 ggggattatc ctgtcgctga ttatgaccgt gccgccggtt atcagccatg ccgacggttc
    12901 aaagccaaag atgggcttcg ccgccatgtt cgatcgcgcg gcattgccgt ttgccatggt
    12961 tacattcttt atgacctttt cgtatgccgg ggtttctgcc tttctggcgc tttacgcccg
    13021 cgaacttaat ctgatgtcgg cggccagtaa tttcctgctc tgctacgcca tcttcctgat
    13081 gatctgccgt accttcaccg gcaatgtttg cgacaaaaaa ggcccgaaat atgtggttta
    13141 cccctgcctg ctgttcttta cggttgggct ggtggttctc ggctacaccc agggcagcgt
    13201 aatgatggtc gtttctggcg cgttgattgg tatcgggtat ggttccgtga cgccagtttt
    13261 tcagacgcag attatcagtt cagtggaacc gcataaaatc ggtgtcgcaa actccctctt
    13321 cttcaatgcg atggatgcag gcctggcgct gggagcctgt gtgatgggga tgatggttgc
    13381 acatactggc taccgaatga tttatctgct gggcgcacta ttagtggtag tggctggtgg
    13441 agtctatgcg ctgcaaatga agggaaaaag cggtgtcgcg ctagtagtgg caaaagaaat
    13501 tcattaatac gacacgtttc attaagattt ttcgcaggta accacctata gtcattgtcg
    13561 ttaataaaaa atgacaggcg atagggtatg gcagtttcag caaagcacga cgaatttaat
    13621 cactggtggg caacagaggg cgactgggtt gaagagccta actatcgccg taatggtatg
    13681 agcggggtgc aatgcgtcga gcgcaacggc aaaaagctat atgtaaagcg catgacgcat
    13741 catctgtttc attccgtacg ttatcctttc ggccgaccaa cgattgtccg tgaggttgcg
    13801 gtaattaaag aactggaacg ggccggtgtc atcgtgccga agatcgtttt tggtgaagcg
    13861 gtgaaaattg agggggaatg gcgcgcgttg ctggtgactg aagatatggc ggggttcatc
    13921 agcattgctg actggtatgc ccagcatgca gtaacgccct attctgacga agtacggcaa
    13981 gccatgttga aagcagtggc gctggcgttt aagaaaatgc atagcgttaa tcgtcagcat
    14041 ggctgttgtt atgttcgcca tatctatgtg aaaacagaag gtaaggcaga agctggtttt
    14101 cttgatctgg agaaaagtcg tcgccgcttg cgtcgcgata aagcgattaa tcatgacttc
    14161 cgccagctgg aaaaatatct ggagccgatc cccaaagcgg actgggagca ggtaaaagcg
    14221 tattactacg ccatgtaatc gttgtaggcc agataatacg cgtaagcgtc gtatctggcg
    14281 ctagtacccg atcacaactc gatttcaata tcgccttttg ctcggcaaca acagggcaaa
    14341 atttcccccg gctgaataaa ggctaacggt tcggcaatcc agtccacctg gcctgcaacc
    14401 aggcgtgtgc gacaggagcc gcagtaacct tcgcggcact ggtactcaac cgccacattg
    14461 tgggattcca gcgccgccag aagggaaggg tgttcatcct ggcacagcag ttgtgtgcca
    14521 gtgatgcgca gggtaacgcg agccatcaga gctggaagtt actcaaatcg tcggtgtcca
    14581 cttccgagtc aatctgcccg accagataag aactgacttc cacttcctgc ggagcaacct
    14641 gcacgttatc agacaccagc caggtgttga tccacgggat cgggttggag cgcgtctgga
    14701 acggcagatc caaaccgact gcctgcatgc ggatattggt gatgtattca acgtactggc
    14761 agagaatgtc tttattcaga ccaatcatcg aaccgtcacg gaacagataa tccgcccagt
    14821 ctttctcttg ctgtgccgcc tggacaaaca ggtcatagca ctcctgctta cattcttcgg
    14881 caatttccgc catctccgga tcatccgcac cgctgcgcag cagattcagc atatgctgag
    14941 taccggtcag gtgcagggct tcgtcgcggg caatcaggcg aataattttg gcgttacctt
    15001 ccatcagttc gcgttctgca aaggcaaagg aacaagcaaa gctgacgtag aagcggatcg
    15061 cttccagcgc gttaacgctc atcaggcaga gatacagttt tttcttcagc tcgcgcaggc
    15121 taacggtcac ggttttaccg ttaacagtgt gggtgccttc gcccagcagg tgccagtagc
    15181 tggtcatttc gatcagctca tcgtagtagc tggagatccc ttccgcacgt ttctggatct
    15241 gctcgttggt gacgatatcg tcaaacacaa cagacggatc gttaacgata ttacgaatga
    15301 tatgagtata ggaacgggaa tgaatcgttt ctgagaacgc ccaggtttct acccaggttt
    15361 ccagttccgg gatcgagatc agcggcagca gtgccacgtt cgggctacga ccctgaatgg
    15421 agtccagcag cgtctgatat ttcaggttgc tgataaagat gtgtttttcg tgttcaggca
    15481 gcgcctggta atcgatacgg tcacgagaaa cgtcaacttc ttccggacgc cagaagaaag
    15541 agagctgctt ttcgatcagc ttttcgaaga tgtcatattt ttgctgatcg taacgggcca
    15601 cgttgaccgg ctgaccaaag aacatcggtt ctttgagctg atcatttttc gtctgtgaaa
    15661 aggtggtata tgccatgagt gtgtcctgtt gggagtttaa tgccggataa ggcattttac
    15721 gccgcatcct gtgcctgatg cgacgctggc gcgtcttatc aggcctacaa atccgagccg
    15781 taggccggat taggcgttca cgccgcatcc tgtgcctgat gcgacgctgg cgcgtcttat
    15841 caggcctaca aaccgagccg taggccggat aaggcgttta cgccgcatcc ggcatctcaa
    15901 tatcagatct tacatgcgcc gctttcgcag ccatcgtcct ggattgacgg caccagatcg
    15961 tcctgcgcgt cttcagcgcc gtcacgggtg ttctgataat acagcgtttt gaccccgaat
    16021 ttgtaggcgg tgagcaggtc tttcagcaac tgctgcatcg gcacttttcc tgacgggaag
    16081 cgtgacggat cgtagttggt gttagcagag atcgactgat cgataaattt ctgcatgata
    16141 cccaccagtt gcaggtagcc atcgttaccc ggcatttccc acagcagctc ataggcgtcg
    16201 tgcagatgct cgtagtccgg caccacctgg cgcagaatac cgtctttcga cgctttgatg
    16261 ctgacgtaac cgcgcggcgg ttcaataccg ttagtggcgt tagagatctg cgaagaagtc
    16321 tcggacggca tcagagcaga aagcgtggag ttacgcaggc cgtgtgtttt gattgactca
    16381 cgcagagctt cccagtcgta atgcagcggt tcattagcga tggcatccag atctttctta
    16441 taggtatcga tcggcaggat ccctttcgcg taagtggttt cgttaaacca cgggcacgcg
    16501 ccttgctctt tcgccagctc attagacgct ttcagcaggt aatactgaat cgcttcgaag
    16561 gttttatgcg tcaggttgtt ggcgctgccg tcggagtagc gtttaccatg cttcgccagg
    16621 tagtaagcga agttgatcac accaataccc agcgtacgac gacccatcgc tccacgtttg
    16681 gcggccggga tcgggtaatc ctgataatcc agcagcgcgt caagtgcacg aaccgccaga
    16741 attgccagct cttccagttc atccaggcta ttaattgcgc ccaggttgaa agcagacagc
    16801 gtacacagcg cgatttcacc gttctcgtcg ttgacgtcgt tcagcggttt ggtcggcagg
    16861 gctatctcca ggcacaggtt agactgacgc actggcgcga tggccggatc aaacgggcta
    16921 tgggtattgc agtggtcaac gttctgaata tagatacgac cggtagacgc acgttcctgc
    16981 atcatcagcg agaacagctc aacggctttc acacgctgct tgcggatgct gtcgtctttc
    17041 tcatatttgg tatacaggcg ttcaaactct tcctgatcgg cgaagaatgc gtcgtacagc
    17101 cccggtacgt cggacgggct gaacagggtg atatcttcac ctttcagcag gcgggtatac
    17161 atcagtttgt tgatttgtac cccgtagtcc atatgacgca cgcggttgcc ttccacacca
    17221 cggttgtttt tcaacaccag caggctttcc acttccagat gccacatcgg gtagaacagc
    17281 gttgccgcac cgccgcgcac gccgccctga gagcaggatt tcaccgcagt ctggaaatgt
    17341 ttgtagaacg gaatgcagcc ggtatggaac gcttcgccac cgcgaatcgg gctacccagc
    17401 gcacgaatac gcccggcgtt gatgccgatc ccggcacgct gggaaacata tttcacaatc
    17461 gcgctggagg tggcgttgat ggaatccagg ctgtcgccgc actcgatcag tacgcaggag
    17521 ctgaactgac gagtcggggt acgcacgccg gacatgattg gcgtcggcag cgaaatttta
    17581 aatgtggaaa ccgcgtcgta aaaatgcttc acatattgca ggcgcgtttc acgcgggtag
    17641 ttcgagaaca ggcacgcagc aaccagaata taaaggaact gggcgctttc gtagatttcg
    17701 ccggtcacac ggttctgtac cagatatttg ccttccagtt gcttaacggc agcataagag
    17761 aaggtcatat cacggtcgtg atcgataaac gtgtccatct gcttgaactc ttcttccgtg
    17821 tagtcttcca gcagatgatt atcgtatttg cccatctcga ccattttcac cacgtggtcg
    17881 tacagcgcag gcggctcaaa ctggccgtag gcttttttac gcaggtggaa gatcgccagg
    17941 cgcgcggcga gatactgata atccggcgca tcacgggaga tcaggtctgc ggcagccttg
    18001 ataatggttt cgtggatgtc agaggtcttg ataccgtcat aaaactgaat gtgggagcgc
    18061 agctcgacct gggaaatcga aacgttatgc agtccttctg ccgcccaatc cagaacgcga
    18121 tggattttgt cgagattgat gcgctctgtg ctaccgtcgc gctttgtcac cagcagattc
    18181 tgattcatgt atgtcgtacc tgtttttgga attctttccg caatagtttc gtgcagacta
    18241 cctgtagtac gggaagctgc ttagaacact atatataggg ggtatgtttg ggattcactg
    18301 caagatagtg tgaaaatggc cctcttgcaa gtgcataact ttgtggataa ctcaggaagg
    18361 aaaaagtggc tttcgcgcac cttaggtcag acaaggtgtc cgggaaagtc aatgggaaga
    18421 gaaaaatttg ttataaataa ctgttcggcg atttcttgaa cggcagaata ggttggataa
    18481 attgatatag atggtttatt aatctttttg ctaatgattg caaaaattga tctaagcacg
    18541 agtcatcaca taatctgatg tatcagttgt ggtcaatata taagcgaatg taagcgattg
    18601 ttgatgtcga aat
//
LOCUS       DABSYT020000086        18559 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.086, whole genome shotgun sequence.
ACCESSION   DABSYT020000086 DABSYT020000000
VERSION     DABSYT020000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.086"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(103..366)
                     /gene="cedA"
                     /locus_tag="ID725_RS17890"
                     /old_locus_tag="pgaptmp_003578"
     CDS             complement(103..366)
                     /gene="cedA"
                     /locus_tag="ID725_RS17890"
                     /old_locus_tag="pgaptmp_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGV7852091.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            549..2810
                     /gene="katE"
                     /locus_tag="ID725_RS17895"
                     /old_locus_tag="pgaptmp_003579"
     CDS             549..2810
                     /gene="katE"
                     /locus_tag="ID725_RS17895"
                     /old_locus_tag="pgaptmp_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGV7852092.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2857..3615)
                     /gene="chbG"
                     /locus_tag="ID725_RS17900"
                     /old_locus_tag="pgaptmp_003580"
     CDS             complement(2857..3615)
                     /gene="chbG"
                     /locus_tag="ID725_RS17900"
                     /old_locus_tag="pgaptmp_003580"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGV7852093.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3628..4980)
                     /gene="celF"
                     /locus_tag="ID725_RS17905"
                     /old_locus_tag="pgaptmp_003581"
                     /gene_synonym="chbF"
     CDS             complement(3628..4980)
                     /gene="celF"
                     /locus_tag="ID725_RS17905"
                     /old_locus_tag="pgaptmp_003581"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGV7852094.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNGVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5085..5927)
                     /gene="chbR"
                     /locus_tag="ID725_RS17910"
                     /old_locus_tag="pgaptmp_003582"
     CDS             complement(5085..5927)
                     /gene="chbR"
                     /locus_tag="ID725_RS17910"
                     /old_locus_tag="pgaptmp_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGV7852095.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5935..6285)
                     /gene="chbA"
                     /locus_tag="ID725_RS17915"
                     /old_locus_tag="pgaptmp_003583"
     CDS             complement(5935..6285)
                     /gene="chbA"
                     /locus_tag="ID725_RS17915"
                     /old_locus_tag="pgaptmp_003583"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGV7852096.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6336..7694)
                     /gene="chbC"
                     /locus_tag="ID725_RS17920"
                     /old_locus_tag="pgaptmp_003584"
     CDS             complement(6336..7694)
                     /gene="chbC"
                     /locus_tag="ID725_RS17920"
                     /old_locus_tag="pgaptmp_003584"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGV7852097.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7779..8099)
                     /gene="chbB"
                     /locus_tag="ID725_RS17925"
                     /old_locus_tag="pgaptmp_003585"
     CDS             complement(7779..8099)
                     /gene="chbB"
                     /locus_tag="ID725_RS17925"
                     /old_locus_tag="pgaptmp_003585"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGV7852098.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8399..8737)
                     /gene="osmE"
                     /locus_tag="ID725_RS17930"
                     /old_locus_tag="pgaptmp_003586"
     CDS             complement(8399..8737)
                     /gene="osmE"
                     /locus_tag="ID725_RS17930"
                     /old_locus_tag="pgaptmp_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGV7852099.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8939..9766
                     /gene="nadE"
                     /locus_tag="ID725_RS17935"
                     /old_locus_tag="pgaptmp_003587"
     CDS             8939..9766
                     /gene="nadE"
                     /locus_tag="ID725_RS17935"
                     /old_locus_tag="pgaptmp_003587"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGV7852100.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            9996..10883
                     /gene="cho"
                     /locus_tag="ID725_RS17940"
                     /old_locus_tag="pgaptmp_003588"
     CDS             9996..10883
                     /gene="cho"
                     /locus_tag="ID725_RS17940"
                     /old_locus_tag="pgaptmp_003588"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGV7852101.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSQEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10843..11418)
                     /gene="ves"
                     /locus_tag="ID725_RS17945"
                     /old_locus_tag="pgaptmp_003589"
     CDS             complement(10843..11418)
                     /gene="ves"
                     /locus_tag="ID725_RS17945"
                     /old_locus_tag="pgaptmp_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGV7852102.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11621..12106)
                     /gene="spy"
                     /locus_tag="ID725_RS17950"
                     /old_locus_tag="pgaptmp_003590"
     CDS             complement(11621..12106)
                     /gene="spy"
                     /locus_tag="ID725_RS17950"
                     /old_locus_tag="pgaptmp_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGV7852103.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12436..13404)
                     /gene="astE"
                     /locus_tag="ID725_RS17955"
                     /old_locus_tag="pgaptmp_003591"
     CDS             complement(12436..13404)
                     /gene="astE"
                     /locus_tag="ID725_RS17955"
                     /old_locus_tag="pgaptmp_003591"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGV7852104.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13397..14740)
                     /gene="astB"
                     /locus_tag="ID725_RS17960"
                     /old_locus_tag="pgaptmp_003592"
     CDS             complement(13397..14740)
                     /gene="astB"
                     /locus_tag="ID725_RS17960"
                     /old_locus_tag="pgaptmp_003592"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGV7852105.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14737..16215)
                     /gene="astD"
                     /locus_tag="ID725_RS17965"
                     /old_locus_tag="pgaptmp_003593"
     CDS             complement(14737..16215)
                     /gene="astD"
                     /locus_tag="ID725_RS17965"
                     /old_locus_tag="pgaptmp_003593"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGV7852106.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16212..17246)
                     /gene="astA"
                     /locus_tag="ID725_RS17970"
                     /old_locus_tag="pgaptmp_003594"
     CDS             complement(16212..17246)
                     /gene="astA"
                     /locus_tag="ID725_RS17970"
                     /old_locus_tag="pgaptmp_003594"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGV7852107.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17243..18463)
                     /gene="astC"
                     /locus_tag="ID725_RS17975"
                     /old_locus_tag="pgaptmp_003595"
     CDS             complement(17243..18463)
                     /gene="astC"
                     /locus_tag="ID725_RS17975"
                     /old_locus_tag="pgaptmp_003595"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGV7852108.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
BASE COUNT         4705 a         4984 c         4395 g         4475 t
ORIGIN      
        1 tcaaaggcag ctttttgtta caggtggttt gaatgaatgt agcaatgaaa tagagaattt
       61 caggccatgt acctcccggc aaaaccggga ggtacgtaat ctttactcag tcacttcccc
      121 ttcctggcgg atctgatttg cccaacgctg ggcggattca ggcacagtaa acgccggtga
      181 gcgcagaaat gactctccca tcagtacaaa cgcaacatat ttgccacgca gcatccagac
      241 atcacgaaac gaatccatct ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac
      301 atagctaata atctggcggt tttgctggcg gagcggtttc ttcattactg gcttcactaa
      361 acgcatatta aaaatcagaa aaactgtagt ttagccgatt tagcccctgt acgtcccgct
      421 ttgcgtgtat ttcataacac cgtttccaga atagtctccg aagcggggtc tggctggtgg
      481 tctatagtta gagagttttt tgaccaaaac agcggccctt tcagtaataa attaaggaga
      541 cgagttcaat gtcgcaacaa aacgaaaaga acccacatca gcaccagtca ccactacacg
      601 attccagcga agcgaaaccg gggatggact cactggcacc tgaggacggc tctcatcgtc
      661 cagcggctga accaacaccg ccaggtgcac aacctaccgc ctcagggagc ctgaaagccc
      721 ctgatacgcg taacgaaaaa cttaattctc tggaagacgt acgcaaaggc agtgaaaatt
      781 atgcgctgac cactaatcag ggcgtgcgca tcgccgacga tcaaaactca ctgcgtgccg
      841 gtagccgtgg tccaacgctg ctggaagatt ttattctgcg cgagaaaatc acccactttg
      901 accatgagcg catcccggaa cgtattgttc atgcacgtgg atcagccgct cacggttatt
      961 tccagccata taaaagctta agcgatatca ccaaagcgga tttcctctca gatccgaaca
     1021 aaatcacccc agtatttgtt aggttctcta ccgttcaggg tggtgctggc tctgccgata
     1081 ccgtgcgtga tatccgtggc tttgccacca agttctatac tgaagagggt atttttgacc
     1141 tcgttggcaa taacacgcca atcttcttta tccaggatgc gcataaattc cccgattttg
     1201 ttcatgcggt aaaaccagaa ccacactggg caattccaca agggcaaagc gcccacgata
     1261 ctttctggga ttatgtttct ctgcaacctg aaactctgca caacgtgatg tgggcgatgt
     1321 cggatcgcgg catcccccgc agttaccgca ccatggaagg ctttggtatt cataccttcc
     1381 gcctgattaa tgccgaaggg aaggcaacgt ttgtacgttt ccactggaaa ccactggcag
     1441 gtaaagcctc actcgtttgg gatgaagcac aaaaactaac cggacgtgac ccggacttcc
     1501 accgccgcga gttgtgggaa gccattgaag caggcgattt tccggaatac gaactgggct
     1561 tccagttgat tcctgaagaa gatgaattca agttcgactt cgatcttctc gatccaacta
     1621 aacttatccc ggaagaactg gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca
     1681 atccggataa cttctttgcc gaaaacgaac aagcagcatt ccatccaggt catattgttc
     1741 ctggtctgga cttcaccaac gatcccctgt tgcagggacg tttgttctcc tataccgata
     1801 cacaaatcag tcgtcttggt gggccgaatt tccatgagat tccgattaac cgcccaacct
     1861 gcccttacca taatttccag cgtgacggca tgcatcgtat ggggatcgac actaacccgg
     1921 cgaattacga accgaactcg atcaacgata actggccgcg cgaaacaccg ccgggaccga
     1981 aacgcggcgg ttttgaatca tatcaggaac gcgtggaagg aaataaagtt cgcgagcgca
     2041 gtccatcttt tggcgaatat tattcccatc cgcgtctgtt ctggctaagt cagacgccat
     2101 tcgagcagcg ccatattgtc gatggtttca gttttgagtt aagcaaagtc gttcgtccgt
     2161 atattcgtga gcgcgttgtt gaccagctgg cgcatattga tctcactctg gcccaggcgg
     2221 tggcgaaaaa tctcggtatc gaactgactg acgaccagct gaatatcacc ccacctccgg
     2281 acgtcaacgg tctgaaaaag gatccatcct taagtttgta cgccattcct gacggtgatg
     2341 tgaaaggtcg cgtggtagcg attttactta atgatgaagt gagatcggca gaccttctgg
     2401 ccattctcaa ggcgctgaag gccaaaggcg ttcatgccaa actgctctac tcccgaatgg
     2461 gtgaagtgac tgcggatgac ggtacggtgt tgcctatagc cgctaccttt gccggtgcgc
     2521 cttcgctgac ggtcgatgcg gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca
     2581 acggcgatgc caactactac ctgatggaag cctacaaaca ccttaaaccg attgcgctgg
     2641 cgggagacgc gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt gaagaaggga
     2701 ttgtggaagc tgacagcgcc gacggtagtt ttatggatga actgttaacg ctgatggcag
     2761 cacaccgcgt gtggtcacgc attcctaaga ttgacaaaat tccggcgtaa atatggataa
     2821 ggtcacgtcc cctggggagt gaccttacca ccttacttac acatcaagat aactacccaa
     2881 ccgataacca cgctcagcaa ttgcatattt caacgacgct gatgtcagca cttccagctc
     2941 cgttaaccgt gggaaacagt aagcactctg gcgaatcgta ttatcgacaa acgccggatg
     3001 gcacatgact tccagcgagc gttccccgcg atggctggaa tcatcaagca cttgcaggaa
     3061 cagtgcctcg ctaatctctt caccataaaa tgcgctactg aatccgtgcg aactgcgcag
     3121 attagccggt aaatcaccag cgttagatag cggctgacgg tcgatgcgca acgcaatccc
     3181 ctcttcagcc gcaaacctgg caacaatagg gaaaatctgc gggaacatat gcacatgatg
     3241 atggctatca agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact
     3301 ggcaagctcc tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat
     3361 ccattttccc agcacaccat cgcgggttaa ccccggcata gctgtcagtg gcttgcccat
     3421 agtaaggaca aagttcatcc ctatggccag actcggttca tcacgactca actgcaccgc
     3481 atggtcaata gcctgcccat tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca
     3541 ggcctcgata atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat
     3601 cagtaagcgt tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc
     3661 ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc
     3721 atcgcgatcg gaatgcacca acggactaag gtttagcgcc agtaacacac cgttaaattc
     3781 tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc
     3841 catcacttta tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag
     3901 cgtacaggtc atttctaccg cccagtctgc cggaatatta tcaatatgcc catgatgcgg
     3961 gatattaacg taatgttctg cttgcttatc gttgtagata gcgttgatca cttcgcacgc
     4021 tgcatcagag taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc
     4081 cgggttttta tacagctcaa aaagttgttt ctcgactttc tgtactacct gcgctcgtgc
     4141 gccgcctttg tagtattcgc ccatttcaat agccagcatc tctttttgct tgaagtaata
     4201 aagcaaataa gaacacggca acagattcag agaacgaatt aagccttcgc taaatggcag
     4261 atcgaaaata tttttaacgc cagatgcttt taactgcccg gatgccacac catcaagcaa
     4321 ttcggcaaag cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag
     4381 gccgaacaga tcgatagata aatcatcgct gtctttcagc atcagaacat cgcgaataaa
     4441 catcttcatg ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg
     4501 ataaacggct tcagtgacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg
     4561 acaaagttct tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt
     4621 aaacagaccg cccgcgccgt tggtttcctg accaagataa ccatgactta atggaatacg
     4681 ttcatccagt tcacgcgccg gtaattggcc aacgcgcagt tgggtagtaa cgaaatcagc
     4741 atctttcaat gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt
     4801 atcaatcatc cgttggcaga gatcgaaaat aatatccagt ttagctttgc caccttcgac
     4861 atccaccagc cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag
     4921 taactccggg gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat
     4981 aatttctccc ttcagtacag aatactgata tctggcatat ctgccccccc ggacataaat
     5041 aatccagcaa caggacagat atgtgaattg tcaggtataa cgacttactg attaaattca
     5101 gtcaatttct tacgatagct cttaggcgta aaggacgtta atttcttaaa cgttttaata
     5161 aacaaactcg ggctactata accggcctca aacgcaatat ccgtcactga atagttggtc
     5221 atttccagtt gttttttggc aaaattaata cggatttcat taataatctg cattggcgtt
     5281 ttgccataat atcgttgagt cgcccgcgtc aaatattcct gtgatttggc tgacaacgtc
     5341 accatattct ccagcgccga ttcactaaac tgctctttat catgcatctt ttctaccgta
     5401 cttttcagcc actgcggtac atcatcaatc acctgttctt cgcggtaatg acgtaaacgg
     5461 ttaatgacat aaaaagtaac catctcaaca aactcttcca gccctgtttc gcggaaattc
     5521 aatgaagaaa tcactgtttc cacataggtg agaaaagcgt tattggtccg gtatacctgc
     5581 gaagctacaa agcaataagg caacaatggc aggtaatgct gctcaaaaaa gcgtttactg
     5641 atcccaacgt tcaatatgcg cgtggcacca aactcataaa aactttgatg gtgcgaacct
     5701 aacggaataa aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt aatctcctgg
     5761 aaataacgcc cggttaatac cagagtaaat tcataatagt cgtgctgatg cagtccgctg
     5821 atactctcag ttttgttata gataaacaca tggaagtttt tgccattaaa caactgctgt
     5881 tctcgggcag tggcaatttc cggcgcgtta atcactggct gcatcatcga ctccttatgc
     5941 cttcagtttt tcatgaagct caattaattc agtaatcagt tcacgcgcaa gcatggacgt
     6001 cattaaatga tcctgagcgt ggaccagcac cagactcact ttcatcttac cttcgcccgc
     6061 atcgccttca atcagtttcg tctgtaccag atgcgcttca ttcaatgcca tccgtgactg
     6121 atccatcatg gcttttgctg cggcaaaatc gccctgcttc gcctgtttca gtgcggcata
     6181 cgccaggctg cgcgcttgtc cggagttgat gataagcccc atcaccactt cttccagctc
     6241 ttcagcttcc gtttgcgtat cgggaatatt atcgagatcc atcatacatc gttcctctct
     6301 tttcttaccg gcacgattac ccgtaccggc atcgattaaa atttcagggc gttagcgata
     6361 tcttcttcgc tctcttcttt atcaatcgca ttttgtgctt tgttagccac cacaacaaag
     6421 ggcagataaa ttaacgttgc gataccaagg ttgaagagtg caaccagcaa tgcgacgacg
     6481 ctaccgttgg tgttaaagaa ggctcccaga ccggttggca tggtccacgg tgcaatattg
     6541 gtcaccggag gaataatgcc catgtagtac gctgccaggg tgattgccgc cagaatcggt
     6601 tgtaccagta caaacgggat aaacatcacc gggttcatga taattggcag accaaacaga
     6661 atcggttcgt taatctggaa gatgccggac ggcagcgcca gttttgccac ctgacgataa
     6721 tcagcacggc gagaggcaat aaagatagcc aggatcaggc ctaaagtcgc accactgcct
     6781 ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt cttaccggct
     6841 gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc cagcgcccac
     6901 ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat accgaagaac
     6961 cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa cgatgccagt
     7021 ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt attcaacgcc
     7081 caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc cgagaaggaa
     7141 cgcgatactg aagcaggtac gctgtcgggc agtttaatga cccaattgcg gcggacaata
     7201 aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat attcgcccca
     7261 cctaaccagt ttgcgccaac cgcataggcc tcaccgacac tatatggggt gacggtcata
     7321 aatgctgcaa cggataacaa cccagccgcc agcgcatcga ctttacgctc ttctgccagc
     7381 gccatgccaa taaagaacgg tgccattaaa gacattatcc ctaatgttcc gttatatacg
     7441 ttgccgccaa tacctttcag accattaagt gtttcaatgg ttgaggcgtc gaggcgaata
     7501 cctaaggaat aaaaaaacga cccctcccca aagcttagaa aaacgttgtt aattaataca
     7561 aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta
     7621 acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc aagcgatgca
     7681 ataacattac tcatagaaaa atacccttaa aaaccgcaat ttaaatattg cggtattgat
     7741 ttatgaaata actctttgac gggaaaattt aaaataaatt aatttgctgc ggctttttta
     7801 atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga ctttgccata aagcagcgag
     7861 tcaattactt caaccggttt gttgggtaac aaacgctgga tttcgggcaa catataagca
     7921 atctgcggcc ctaataacac gacatcggca ttctgacctt tttcaccagc cagtgtttcc
     7981 ggaaatgctt caataatgac cggaacttca tatttttctg cctgtgcgcg catttttgat
     8041 accagtaaag aggtagacat gcccgcagaa caaaacagat aaatgtgttt cttttccata
     8101 aaactgccct catcgacgat tatctgtcag ccagacactc cgcaagcctt aacctgcttc
     8161 catgctctgg gtaacttgcg aaaccaacat gacttttttt gtggctgaaa ggagtatacg
     8221 gtatagacct gagcaatagt atttctttga ccttataaat tgtctctcct tgacctgcgg
     8281 ttatgaccct cttcacattt cgggcaaata attcgcgaag ataattaaga actcaggcca
     8341 aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca agctttcaga cgaagtgatt
     8401 acttcgcagc ctgtggatca gtgtcgtatt cagcacaggt ctgataaccg gagttgatga
     8461 catgtccggt atcatctaac gcgacaaagt aggtttctgc tttaccatca cgttgaccca
     8521 ggatgtaggt ctggcaagta ccacgagcat ggatcatgct cacttcagac gaaggtttac
     8581 ccgcaatttg tgcaacctgc gcccggctca tgcctttttt gacgtctttc accacaggct
     8641 gtacaaactg gtctttggta cgatcataag ccgtacaacc cgccagcatg gttaataccg
     8701 ccgctgcact cagaattcct gccatattct tgttcatatt ccgtcctatt gtttatcagc
     8761 gtgttagata agcctggaat acattgggcg ctttttcaag cccatgaacg aaacggctcc
     8821 gctttcagag gattcctgta tgacgtttta accaccattc agcccgctgt cgcttgtcgt
     8881 ttcagtagca acgggttagc tttaaggaag ttttgtcttt tctgtctgga ggggttcaat
     8941 gacattgcaa caacaaataa taaaggcgct gggcgcaaaa ccgcagatta atgctgaaga
     9001 ggaaattcgt cgtagtgtcg attttctgaa aagctacctg caaacttatc cgttcattaa
     9061 atcactggtg ctcgggatca gcggcggtca ggactccacg cttgccggta agctgtgcca
     9121 gatggcgatt aatgagctgc gccaggaaac cggcaacgaa tcactgcaat ttattgccgt
     9181 acgcctgccc tatggtgttc aggccgacga acaagattgc caggatgcca ttgcctttat
     9241 tcaaccggat cgcgtattaa ccgttaatat caagggcgcg gtattggcta gcgagcaggc
     9301 attgcgggaa gcatgcattg aactgagcga ttttgtccgt ggcaatgaaa aagcgcgtga
     9361 gcggatgaaa gcacaatata gcattgcggg tatgaccagc ggtgtcgtgg tgggcaccga
     9421 tcatgcagca gaagccatta ccggattctt cactaaatat ggtgacggcg gtacggacat
     9481 taatccactg tatcgtctca acaaacgtca gggtaaacag ttactggcag cattaggttg
     9541 cccggaacac ctttataaga aagcgccaac ggccgatctg gaagatgatc gcccttctct
     9601 gccagatgaa gtggcactcg gcgtgaccta tgacaatatc gacgactatc tggaagggaa
     9661 aaacgtacct caacaggtcg ccagaacaat agagaactgg tatctgaaaa ccgaacataa
     9721 acgccgtccg ccaattaccg ttttcgatga tttctggaaa aagtaataat ttgcacctat
     9781 tggattgtgc aaaaaagagt aatttgttca cgccggatgc ggcgtgaacg ccttattcga
     9841 cctacaaaac tatgcaaatt caatacattg caggagtcga ataggcctga caggcgtagc
     9901 acgtcagacg gtgtaacctt tgtcatcgac ccgcctcttt tttaatcgct tcccggctgt
     9961 tacactggat agataaccag cattcggagt caacagtggt acggcgttta acttctccgc
    10021 ggctcgaatt tgaagctgcg gcaatttatg aatatcccga acatttacgt tcattcctta
    10081 atgacttacc cacccgacca ggggtgtatc tgtttcatgg cgaaagtgac accatgccgc
    10141 tctatatcgg caaaagcgtt aatatccgca gtcgggtgct ttctcattta cgcaccccgg
    10201 atgaagccgc catgctacgg caatcccggc gaattagctg gatctgcacc gcaggcgaaa
    10261 tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca acagccgctg tttaataaac
    10321 ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa tgaaaagcgc gtcgatgtgg
    10381 tgtatgccaa agaggtggat ttttcacgag cccccaacct gtttggcctg tttgccaata
    10441 ggcgcgcagc tttgcaagca ttgcagagca tcgctgatga acaaaaactt tgttatggtc
    10501 tgttgggact ggaaccgtta agtcgcggtc gtgcgtgttt tcgttcagcg ctaaaacgtt
    10561 gcgccggagc atgctgcggt aaagagagcc atgaggaaca tgcgctacgc ttgcgccagt
    10621 ctctggagcg tttgcgggtg gtgtgttggc cttggcaagg ggcggtggcg ctgaaagaac
    10681 agcacccgga aatgactcaa tatcatatta ttcaaaactg gctgtggctg ggggcggtta
    10741 attcgcagga agaagcgaca acgttaattc ggacacccgc cgggtttgat cacgacggtt
    10801 ataaaattct ttgtaagccg ctgctttccg gtaactatga aattactgaa cttgatccgg
    10861 cgaatgacca gcgagccagt tgatttcact gaacaacagt ttaccttgcg gttgtaaaag
    10921 ccgcagggtg tgcctgccat cgaaccagca cgcgccctgg tcggtagtga gcagtttgtc
    10981 gcccagttgc cacgccccgt taatcacaaa caccaccccg ccgcgtgagc caaaggtagt
    11041 aaacgtacgt tcggcaattc tgactttcgc cttacagaca tccaggcgcg tcataatatt
    11101 gaaatccatc gacatctgcc ctgccgtcag tttcgctttt accacctggt ccgccgcaaa
    11161 ggcaaaaggc tgtaacggtt ttaaggtatg gttaaagtgg tctgcgcttt caaggaacat
    11221 ctcgccgcct tccagcaacg tcactatcct ttccatacca ggaaacaaag aaaactcccc
    11281 attcgccgcg atggatgcaa tgctggcacg ccagtaaaaa tcacgttttg ccggtgggaa
    11341 agtgcaaatt tcgcgcgttt caccggcagc gtttcgccac aaattcaccg acattttacg
    11401 catatcaaag tattccatac tcgctcccgg cccgttcttt tcattgttat cgttcaggta
    11461 accgtggatg gttatcggca actcgggtga tttacgttag tggtgatcag gcttttttta
    11521 caactgtttg agaagagaaa agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta
    11581 gtggacaaga ccggcggtct taagtttttt ggctgaaaga ttattcagca gttgcaggca
    11641 ttttaccttt tgccgctgga cgttctgtca gacgcttctc aaaattagca ttaaattgct
    11701 ttttctgttc cggcgtcagg atgttgtaaa ttttgttctg ggtttccatg tgcgccagca
    11761 tgttagcttt gcgctgttct tccatttttg cgatctgcgc ttcagctttt gctttatcga
    11821 aggtatcgct ggcaatgatg tcatgcattg cgcgacgttc ttccagcggc ggacgtttca
    11881 tctggtcacg ctggcctttc atgatttcgc gaatctgctg tttctgcgcg tcggtcaggt
    11941 tcaggtcttt gaacatcatg tcctgatgcg gaccgaactt gcctttgtgg tgcatcatcg
    12001 gcttcgcgtc agccggtgct gcggtagtgg tgtctgcggc atgggccagg ttagccgcgc
    12061 caagagccag ggtagaggca acaaacagtg cagttaattt acgcatattc tatatccttc
    12121 ctttcagtta tttattacgg ctttcttaag tagcgtgccg tgttgacgag attaacttta
    12181 ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta aaaaacactt
    12241 ttgcgccaat tatggagaaa aaaagaaaat ttgatggaga gtgatgagag aatattacaa
    12301 caccatgatt ttgcagagat tatgaagaac tataccggat gactggtgat aaataaagca
    12361 aataaccagg attaatctgt attaatttat aagaaggtaa cttaataccc gcagaaagat
    12421 ttctgcgggt aagtattagc ttattttttc gagcattaat cccgcgcgta atcccagcgc
    12481 taccaacgga ttagggaata acacatactc tacatcatgg gttacggtaa aacgttcctc
    12541 accgtcctgc gccagcaacg ttcctttctt aaacggcata aaattcagcg tgtcacttgc
    12601 catatgcatt tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc gataacggag
    12661 cggcggtgtt ctcacgatac cgacactctc accagatagc agcgcagcga ttgcgctggc
    12721 agttactgca aactggcgaa gatcgttttg cccaaagggc aacgctttgc caagttccag
    12781 cgtacaggcc agcgcgccaa aatgtctggc gctgaaatgg gtaaacgtac caccaggctc
    12841 ctgatggaac accagcgcct ccagccccgc cgcacccagc cacgtcagga atttctcgtc
    12901 ccagggaatg tcgcgttgcg gtaatacacc gaactgcgga tgcaaggagc cacgaattgc
    12961 ggtatgtaga tcaaggtgcc agcgcacaga ttctttgccc tggtcataaa aatcttccag
    13021 gcactgttcc agttcgcgcg cccgacaggt ttccccgctt tcagcaaaca gctgccaacg
    13081 accgccaaac attcggttca tatcgctatg gcaataacgt ttcccttgct tcagcgcagg
    13141 aggattcccg aggatcacca gcaaccgcca ccgtaacggg atctcgccgt gagatatcgc
    13201 gccaagcaat gcatcaagca tctccacagg tgccgtctca ttaccgtgga tccccgcaga
    13261 aatcaccaga gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac catcgcccag
    13321 ccagcgccag cgaacgccgt tgatttctcg ctcggtgatg acaggttttt tactcgttaa
    13381 ggtcagagca agaaaattat ccattgcccc ctccctcgcg ctggaacgga taaaccgaac
    13441 cgagattcag taattggctc aatacatcca gtgcttcccg cccttcgcgc agcaattgcg
    13501 ggtcggccag atcggcagca gtaagacgat cgcggtagta acgatccacc cagtcattga
    13561 gcgcattaaa caaggtatcg ttcatcatca ccgccggatt caccgcccgg cgttcttctt
    13621 gtgtcaatac cacccgcaac cgcaggcacg ccgggccacc gccattcgcc atgctttcac
    13681 gtaaatcaaa gacttttagt tcgctaatcg ggttgtcagc ggcaaggagt tcattgagat
    13741 aaccccatac tccggcgtgt tcccgacact cctgaggcag caccaacatc atggaaccat
    13801 catcgcggct caacagttgg ctgttaaaca gatatgtaga caccgcatcc gacacggaaa
    13861 cctgagttgc cggaacttct atcgccataa aaccgttcac ccgcgcacgc aggtttgcca
    13921 gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg cggttactca
    13981 cggcaatcac gtcattatga aaaacgccct ggtcgataac gtccgggttt tgctgggcga
    14041 aaatcacctg ttggggattc acctgattca gccttgccac cgcctcgctg gcttcgcgag
    14101 tctgtcgcgc cggataacgg gaaggccggg tatcattgcc ctcttctcgc ccgtagacaa
    14161 aaagttgcat acccggttca ccgtaatgac cgccgagacg attgtggttt gccgccccct
    14221 catcaccgag caaggctacc tgcggcaacg ccgaatggac actaaatttc tcttcgtcgt
    14281 taaaaatcgc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg tgaaatttat
    14341 tgttcaggtt agcaacggtg agatgcactt tgccatccag cgtatcggca gatggcgcga
    14401 tcgttgccgc attggctacc cacattggcg aagcggaact gacgctggaa agccagtgcg
    14461 gtgcctggcg tacaaccttt tccagtacct gctcatcgct accactgaat cccaactgac
    14521 gcagcaccgg aataaacgga cgctcgtgag gcgggatcac ggcctggggg aatcccgcat
    14581 cggcaagggt tttcattttc agtaagccct gttttgccgc cagtcgcggg ttagagacct
    14641 gaaaacggtg acgggtagag gctttattac caaacgacag gcccgcgtaa tgatgcgtca
    14701 gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct catcggaaaa
    14761 atccagcccg gggttaagcg ttgcgggcaa agttaacgag tccgactcca ggctcgccat
    14821 aggccatgcg cagtaatctg cggcatacca ggcgctgggg cgatggttac cggatgcacc
    14881 aatgccgccg aatggcgcgg tactggcagc accagtaagc ggtttgttcc agttaacaat
    14941 ccccgcccgc gcctccagca acagttgatc gaatttttcc cgctcggggg aaaccagacc
    15001 gcaagagagt ccgaagcgag tgttattcgc cattcgaatc gcttcctcga aagaatcata
    15061 acgccagacg cgcaataacg gtccgaacac ctcttcatct ggtacgccag caacgcctgt
    15121 catttcaatg atccccggcg tcagcaacga tgtccctgct tgtaataagc gcggcgcaag
    15181 cagggttcgt ccacccatcg cttccagttg ctgccaggca gtaaacacct gctgtgcggc
    15241 ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt tgcccggcgt
    15301 taatcgctgg ctgacggcaa ccagacgagc aagaaatgca tcgccctgcg ccccgctttt
    15361 cagcaataca cggcgggcgc aggtgcagcg ttgcccggct gtaacaaacg ccgactgaat
    15421 ggtcagatgg acagccgcgt cgatatccgc cacctcatcg ataatcagcg ggttattgcc
    15481 gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat gcagctggta
    15541 gccggtattg gcgctgccgg taaacagcaa gccgtcgaga tcctccagcg cactcagcgc
    15601 ctgacccgtt tcacacccgc cctgcaccag gttcagcacg cctggcggca agccagcctg
    15661 ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg gtttaaagat
    15721 aatggtgtta cctgccagca atgcgggaac gatatgtccg ttcggcaaat gaccagggaa
    15781 attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc tcgccgcacc
    15841 gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa ttgatatcgc
    15901 gattttattg atcatcgccg tcaattcggt tgccgcctcc cagcgcggct tgccggtttc
    15961 tcgggcaatg attgcggtta attcgccttt attcctttcc agcaatccgg caaagcgttc
    16021 gacaacggcc tgacgttcag caaatgagag ccgcgcccag cgcggaaacg ccgcacgggc
    16081 tgcccgacaa gcctgcccga cctgagcggc atcggcatca ttgccttgcc ataacacctc
    16141 gcccgatacc ggattacgct tcacacgcga tgcaccctgg cccgttatcc agtcaccgtt
    16201 aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg aacgcgatcc
    16261 cctgcatggc atttgagggc atccagttgt gcggcggtta aaatcaaacg ctcggttgcc
    16321 ggatcggtac gcaccagcac cacgcggaaa tggtgataat tttcattggc gaccaggcag
    16381 gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg acttttacgg
    16441 atggcgcgca cgcggtcgat gtcacactca agcgtcggcc caccgtcgaa gatgtcgata
    16501 tagttgcggt agcgaaaacc ttctttctcc agcaccgcgc gggcaggcgc ggtttgcgga
    16561 tgtacctgac cgatgacgtc ctgggcttcc tgggataaaa agtgcgtata gatcggatgt
    16621 ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa atcggcgcgg
    16681 ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga atagccgtgt
    16741 tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc gcgaaaagcc
    16801 gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt gcgccagtcc
    16861 gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact gagaaacaac
    16921 gtcggcaatg cgttatagac attcagctct tttgaggcgt gaaccaacgt gccgacgcga
    16981 tagttgtacc agggatcgtt cagcccaact gccacctcaa tggcgcaaat ccccgccacg
    17041 gtgcctgtct cgctatcttc cagtacaaac acataaccct gctcactttt gggcagttca
    17101 ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc attggcggga
    17161 agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga gacatctgag
    17221 cgctcaacgg gacggatgac catcatgatg aacctcggct aacaaagtgt tcgcaagcaa
    17281 ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc gcaggcgcaa
    17341 aacgcaccac gttgccaccc gcaatcagta ccatcacgcc tgctttcgcc gcttcctgag
    17401 agatctgttt cgcttgcccg gcgtaatcgg cattcagtac acagccaatc agcaaaccta
    17461 agccgcgaac ttcattgaac aaaccatagc ggtgattaat aatattaaga cgctcaacaa
    17521 accagtcgtg acgttgctta accccgttaa gcatctctgg cgtattgatg agctccagca
    17581 ctttgcctgc caccgccgag gccagcggat tgccgccata ggtggtgcca tgagtgccaa
    17641 cggtcatcac gctggcgcac tcttcggttg ctaacaacgc accgaccggg aaaccgccgc
    17701 ccagagcttt ggcggtagtt aacagatcag gcgtcacgcc gtagtgcata taggcataca
    17761 gttccccggt acgcccgacg ccggtttgta cttcatcaaa aatcaacagc gcattgtggc
    17821 gatcacacaa ttcacgcaga ccttgtaaaa atgcgttgct ggctggcacc acaccgcctt
    17881 caccctggat gggttcgaca atcaccgcac aggtagagtc gtcaatcagc gcgctggcag
    17941 agttaatatc gttatatgca gcatgacgga tatctggtgg cagtggcgca aaatcctgtg
    18001 aataggctgg ctgcccaccc gcactaacag taaacagcgt gcgaccatga aacgcatttt
    18061 taaacgccac gataccgctc ttatggctgc cgtagcggtc gtgagcgaat ttacgcgcca
    18121 gttttagcgc cgcttcgttg gcttccgcac cggagttaca aaagaaaacg cgatcggcaa
    18181 acgtggcgtc gatcaatttt ttcgccagtc gcagtaccga ctcgttggtg taaccgttgc
    18241 cggtatgcca gaacttactc gcctgttcgt tcagcgcctc acgcagttcc ggatgcgcat
    18301 ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc ccctgctgat
    18361 cccacaagcg cgaaccttcg ccacgtaccg gtataaacgg tgccggagcg taaacaggta
    18421 tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc tctacagtaa
    18481 atagctcgtt atttatatgt taataataag taatgttttc gctgtaaatg tagattgcag
    18541 ggttcgtgcc agccagtga
//
LOCUS       DABSYT020000087        18136 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.087, whole genome shotgun sequence.
ACCESSION   DABSYT020000087 DABSYT020000000
VERSION     DABSYT020000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.087"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            158..2629
                     /locus_tag="ID725_RS17980"
                     /old_locus_tag="pgaptmp_003596"
     CDS             158..2629
                     /locus_tag="ID725_RS17980"
                     /old_locus_tag="pgaptmp_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGV7852109.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEGAASFVPVETESDPM
                     NVAFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RVHRQHTENSAEKIPPVTAVIRREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQ
                     PEPVKVTADEVKKIMQAANISQPDANQLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHS
                     IKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPNQADF"
     gene            2694..2942
                     /locus_tag="ID725_RS17985"
                     /old_locus_tag="pgaptmp_003597"
     CDS             2694..2942
                     /locus_tag="ID725_RS17985"
                     /old_locus_tag="pgaptmp_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGV7852110.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            2920..4050
                     /locus_tag="ID725_RS17990"
                     /old_locus_tag="pgaptmp_003598"
     CDS             2920..4050
                     /locus_tag="ID725_RS17990"
                     /old_locus_tag="pgaptmp_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGV7852111.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(4096..4734)
                     /gene="ompW"
                     /locus_tag="ID725_RS17995"
                     /old_locus_tag="pgaptmp_003599"
     CDS             complement(4096..4734)
                     /gene="ompW"
                     /locus_tag="ID725_RS17995"
                     /old_locus_tag="pgaptmp_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGV7852112.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            4974..5063
                     /locus_tag="ID725_RS18000"
                     /old_locus_tag="pgaptmp_003600"
                     /pseudo
     CDS             4974..5063
                     /locus_tag="ID725_RS18000"
                     /old_locus_tag="pgaptmp_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5091..5834
                     /gene="yciC"
                     /locus_tag="ID725_RS18005"
                     /old_locus_tag="pgaptmp_003601"
     CDS             5091..5834
                     /gene="yciC"
                     /locus_tag="ID725_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGV7852113.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            5864..6403
                     /gene="yciB"
                     /locus_tag="ID725_RS18010"
                     /old_locus_tag="pgaptmp_003602"
     CDS             5864..6403
                     /gene="yciB"
                     /locus_tag="ID725_RS18010"
                     /old_locus_tag="pgaptmp_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGV7852114.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            6508..6906
                     /gene="yciA"
                     /locus_tag="ID725_RS18015"
                     /old_locus_tag="pgaptmp_003603"
     CDS             6508..6906
                     /gene="yciA"
                     /locus_tag="ID725_RS18015"
                     /old_locus_tag="pgaptmp_003603"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGV7852115.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(6946..7665)
                     /gene="tonB"
                     /locus_tag="ID725_RS18020"
                     /old_locus_tag="pgaptmp_003604"
     CDS             complement(6946..7665)
                     /gene="tonB"
                     /locus_tag="ID725_RS18020"
                     /old_locus_tag="pgaptmp_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGV7852116.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            7889..8185
                     /gene="yciI"
                     /locus_tag="ID725_RS18025"
                     /old_locus_tag="pgaptmp_003605"
     CDS             7889..8185
                     /gene="yciI"
                     /locus_tag="ID725_RS18025"
                     /old_locus_tag="pgaptmp_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGV7852117.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            8486..9739
                     /gene="kch"
                     /locus_tag="ID725_RS18030"
                     /old_locus_tag="pgaptmp_003606"
     CDS             8486..9739
                     /gene="kch"
                     /locus_tag="ID725_RS18030"
                     /old_locus_tag="pgaptmp_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGV7852118.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9794..9967)
                     /gene="yciY"
                     /locus_tag="ID725_RS18035"
                     /old_locus_tag="pgaptmp_003607"
     CDS             complement(9794..9967)
                     /gene="yciY"
                     /locus_tag="ID725_RS18035"
                     /old_locus_tag="pgaptmp_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGV7852119.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            10110..11570
                     /gene="cls"
                     /locus_tag="ID725_RS18040"
                     /old_locus_tag="pgaptmp_003608"
     CDS             10110..11570
                     /gene="cls"
                     /locus_tag="ID725_RS18040"
                     /old_locus_tag="pgaptmp_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGV7852120.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            11605..11934
                     /gene="yciU"
                     /locus_tag="ID725_RS18045"
                     /old_locus_tag="pgaptmp_003609"
     CDS             11605..11934
                     /gene="yciU"
                     /locus_tag="ID725_RS18045"
                     /old_locus_tag="pgaptmp_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGV7852121.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(11987..12991)
                     /gene="oppF"
                     /locus_tag="ID725_RS18050"
                     /old_locus_tag="pgaptmp_003610"
     CDS             complement(11987..12991)
                     /gene="oppF"
                     /locus_tag="ID725_RS18050"
                     /old_locus_tag="pgaptmp_003610"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGV7852122.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(12988..14001)
                     /gene="oppD"
                     /locus_tag="ID725_RS18055"
                     /old_locus_tag="pgaptmp_003611"
     CDS             complement(12988..14001)
                     /gene="oppD"
                     /locus_tag="ID725_RS18055"
                     /old_locus_tag="pgaptmp_003611"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGV7852123.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(14013..14921)
                     /gene="oppC"
                     /locus_tag="ID725_RS18060"
                     /old_locus_tag="pgaptmp_003612"
     CDS             complement(14013..14921)
                     /gene="oppC"
                     /locus_tag="ID725_RS18060"
                     /old_locus_tag="pgaptmp_003612"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGV7852124.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(14936..15856)
                     /gene="oppB"
                     /locus_tag="ID725_RS18065"
                     /old_locus_tag="pgaptmp_003613"
     CDS             complement(14936..15856)
                     /gene="oppB"
                     /locus_tag="ID725_RS18065"
                     /old_locus_tag="pgaptmp_003613"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGV7852125.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(15942..17573)
                     /gene="oppA"
                     /locus_tag="ID725_RS18070"
                     /old_locus_tag="pgaptmp_003614"
     CDS             complement(15942..17573)
                     /gene="oppA"
                     /locus_tag="ID725_RS18070"
                     /old_locus_tag="pgaptmp_003614"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGV7852126.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         4757 a         4414 c         4316 g         4649 t
ORIGIN      
        1 gtaactgtta ccctgtcgat aaagttattc accatgataa taacgaaatc ccggcaggtc
       61 tttaaaacag ttccgtaata aacatccgat ttcattctta tatgccagca atggcaggga
      121 tttgttcacc cttaaatctg taatgaggta aaacaaaatg agtaaagtct ttatttgcgc
      181 cgccattccg gacgaacagg caataaagga agaaggtgca gtcgctgtag ccactgccat
      241 tgaagccggt gatgaacgtc gcgcccgcgc aaaatttcac tggcaattcc tggagcatta
      301 tccggctgct caggactgcg cttataaatt tcttgtttgc gaggataaac ccggtatacc
      361 ccgccctgcc ctcgattcct gggatgctga atatatgcag gaaaaccgct gggatgaggg
      421 ggctgcttcc tttgtcccgg ttgagactga atccgatccg atgaacgtcg cttttgacaa
      481 gctggcccct gaagtacaga acgctgtcat ggttaagttc gacacatgtg aaaacatcac
      541 cgttgatatg gtgattagcg cgcaggaatt gttgcaggaa gacatggcaa cattcgacgg
      601 acatatcgtt gaagcgttga tgaaaatgcc agaagttaac gccatgtatc cggagcttaa
      661 gctgcatgcc atcgggtggg ttaagcataa atgtaagcct ggtgccaaat ggcccgaaat
      721 tcaggcagaa atgcgcatct ggaaaaaacg tcgcgaaggt gaacgcaagg aaaccggaaa
      781 atacacgtct gttgttgatc tcgcccgcgc cagagtccac cgacagcaca ctgaaaactc
      841 agcagaaaaa atcccccctg tcactgcagt cattcgtcgc gaatataagc agacatggaa
      901 aacactggat gacgaactgg cctacgctct ctggcctggt gatgtggatg ccggaaacat
      961 tgacggcagc atccatcgct gggcaaaaaa tgaagttatc gacaacgacc gcgaagactg
     1021 gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc cttcgctacg accgccagac
     1081 tatttttggc cttgtccgtg aacgtccgat cgacattcac aaagaccctg tggcactgaa
     1141 caaatacatt actgaatacc tgactacaaa gggcgtgttt gaagatgaag gaacaaatca
     1201 gagcgcaact gacactctct cgtcgccagt accagaaact gatgcagtgg aaacggcaat
     1261 tccggacaac gaaaaaaccg aatgcaaagt ggaagtcgaa ccatctgtag agcgtgaggg
     1321 gccgttctac ttcctcttca ccgacaagga tggcgaaaaa tacggtcgcg caaacaaact
     1381 ttctggtctg gataaggcgc tggctgccgg ggctactgaa atcacaaaag aagaatattt
     1441 tgcccgaaaa aatggcacat acacaggctt accgcaaaat gcaaataccg cacaaaattc
     1501 tgaacaacca gaaccggtaa aagttaccgc tgacgaagta aagaaaatta tgcaggcagc
     1561 caatatcagc cagcctgacg ccaatcagtt gctcgccgca tcacgtggtg aatttgttgc
     1621 agggattagc gacccgaatg atccgaaatg ggtgaagggg attgaaaccc gcgattctgt
     1681 gaaccagaac cagcaagaaa cggaacagaa cgaccagaaa gcggaacaaa acagcccaaa
     1741 tacgcaacaa aacgagccag aaacgaaaca acctgagcca gtagcgcaac aggaaccgga
     1801 aaaagtctgc accgcctgcg gtcagaccgg cggcggcaac tgccctgatt gtggcgcggt
     1861 gatgggcgac gcaacatacc aggaaacatt cgatgaagag tatcaggttg aagttcagga
     1921 agatgatccg gaggaaatgg aaggcgctga acatccacac aaggagaaca ctgacggcaa
     1981 tcagcatcac gatagcgata atgaaactgg cgagacggca gatcactcaa ttaaggtgaa
     2041 cggtcatcaa gaaatcacat ccaccagcag gacgtgtgac catctaatga tcgaccttga
     2101 aaccatggga aaaaatcctg atgccccgat catctcaata ggtgcaatat ttttcgatcc
     2161 gcaaaccgga gatatgggac cggaatttag taagactatc gatctggaaa ctgctggcgg
     2221 ggtcattgat cgggacacca ttaaatggtg gcttaagcaa tcacgcgaag cgcaatctgc
     2281 cattatgacc gatgaaatcc cgttagatga tgcactatta caattgcggg aatttatcga
     2341 cgaaaactcc ggtgaatttt ttgttcaggt ctggggaaat ggagccaact tcgacaacac
     2401 gattttgcgc cgttcatacg aacggcaggg gatcccctgc ccgtggcgtt actacaacga
     2461 tcgcgatgta cgcacaatcg ttgagctggg gaaagccata gacttcgatg ccagaacggc
     2521 tattccattc gaaggtgagc gccataatgc acttgatgac gcccgttacc aggcaaaata
     2581 cgtttcagtt atctggcaaa aactgatccc gaatcaggct gatttttaat gttcaacccc
     2641 ggtcgttgcc caccagctat agtggcggcg accatgatta gcgaacgacg ctcatggcaa
     2701 gacttattct gctcactgag tgggcaaaag aggaattcag tgaaccggtc ccaactccga
     2761 gtacgttaag taaatacgct aaagccggaa tgatatttcc tctccccaaa aaagttggaa
     2821 gacgctggcg agtggatccg caagctcgct ttgtcggaat ggtaaacaag ccggaggtga
     2881 tcgccacaga tcaccctgct ttgaagagga tactggaaga tggcgcgccc gcgaaaatat
     2941 aaaaccgatg ttccgggatt atctccgtat tttgacaaaa gaaataacaa agtttactgg
     3001 cgttacaggc atcccataac aggcaaaaat cacggtctcg gcagtattga ccagaaactg
     3061 gcagaaacta ttgcagcaga agcgaacagc cgtcttgccc ggcagcaaat ggaacaaatg
     3121 ctcagtctgc aggagaaaat tattagtgat accggcggtt catcaaccgt taccattttt
     3181 ctgaataatt acagaaaaat tcaacaggaa agatatgaaa acggcgagat caaactcaac
     3241 acgctgaaac agaaagcggc ccctctcagg gtatttgatg aacgttttgg caccagaccg
     3301 ttagatgcca taaccgtaaa agatgtggta tcagtactgg aagagtacaa ggccagagga
     3361 cataacagaa tgggacaaat tttcaggaaa gtactgatcg atgttttccg ggaagctcag
     3421 caaacgggcg atgtcccgcc aggctttaac cctgcagaat cggcaaaaaa accgcaggtg
     3481 cggatatcaa gacagcgact gacttttgat gagtggatga tgatttataa cgcagcggaa
     3541 aaggatggtt actttttaca gcgcggtatg ctgctggcac tgatgacagg ccagcgcctt
     3601 tcagatattt gcaaaatgca attttcggat atccgggatg gttatcttca tgtcgaacag
     3661 caaaaaacag gaacccggat tgccatccct ctggctctgc gttgcgataa attaaatctc
     3721 accctggatg atgtggtgtc atcctgccgc gattgcgttc ttagtccgtg gctattgcac
     3781 caccatcacg cgaaagggac agctaagcgc ggcgggatgg ttaagccagc aacattaacc
     3841 gttgcattta aaaaagcccg ggattctgtg gattacaact ggcgtgctaa tggcacccct
     3901 ccctctttcc atgagcagag atctttatca gagcgattgt tcagagagca gggggttgat
     3961 accaaaattt tgctaggcca ttcgaatcaa aaaatgaccg atatttacaa cgacgcacgc
     4021 ggtaaggaat ggaaaaaact ggtcatttga tgaccagttt tgcagagggg ttttgcagag
     4081 gttttgcaga gaaaattaaa aacgatatcc tgcggagaac ataaacaccc acggatccag
     4141 tcgtaccgag tcttttacgg tagtaacacc agatttgtac ttagccgtgg tatcgatatc
     4201 catgtaccac actgacatgt taaccaacca gtcacggtta atcagataat caacccccac
     4261 ctgcccggca gctccccagg aatctttcag actgagatcg gaaagccccg cctctttgcc
     4321 atgatcgtta aatccattat caaagaaggt ggtgtagtta atacctgccc caacataagg
     4381 acggaatttg ctgctggcat caccaaaata ccactgcgcc atcagtgttg gcggcagatg
     4441 atgaacggtt gcaatatcgc cggtcgcccg ggtgccgatt ttatggcgga acggcgtcgc
     4501 tgccagtaat tccacaccaa tgttgtcggt cgccatataa gtaaacgtca ggcccagttg
     4561 agtgttattg gtcacgctga atccacccag acttcctaac gtaccaccag caccttctgt
     4621 tggacgtacg gttgcagaac ctgcacgcat aaaaaattcg cctgcttcat gcgcaaaggc
     4681 actgccagag agaagagttg ttactgccaa agccgccact gttaactttt tcatatccgc
     4741 tccgtcgtta tggttataaa aacgtgacga atatacctac attggagtaa caaatgatcc
     4801 tacatagatc acattacata tgataattca ccatttattg atctggatta atttagaagt
     4861 tgcattgaga atcctggtta acgtgattta aatcaatttt taaatatttt cgctacgcaa
     4921 atattaactc gttcgccact tgccgcggtt tgcccacgcc tgaaggccag caattacctc
     4981 cgctttacaa agatatgctt ttccatacaa gacctgatgc tgccacccga ggcttaacca
     5041 gggtacaatt gcccgctaat taacacctgc ataaaactca aggagagtgc atgtctatca
     5101 cggcgcagtc cgtataccgt gacaccggaa atttcttccg taatcaattt atgaccattc
     5161 tgttggtatc gttgctatgt gcgtttatca cagtggtgtt agggcatgtt ttctcaccca
     5221 gtgatgcaca gcttgcgcag ctcaatgacg gcgtgcccgt tagcggcagt agtgggttgt
     5281 tcgacctggt tcagaatatg tcaccggaac agcaacaaat tttgctgcag gcttcagcgg
     5341 cgtccacttt ttcaggatta atcggtaacg ccattctcgc cggaggcgta atattaatta
     5401 tccagctggt gtctgcgggt cagagagtca gtgcgctgcg agctattggt gccagtgcgc
     5461 cgatattgcc aaagttattt attctgattt ttctgactac ccttttagta cagattggca
     5521 tcatgctggt ggtcgttccg ggaattatca tggccatatt actggccctg gcaccggtga
     5581 tgctggtaca ggacaaaatg ggcgtttttg cctcgatccg tagcagtatg cggctgactt
     5641 gggcgaatat gcgtctggtg gcacccgcag tactgagctg gttgctggca aaaacactgt
     5701 tgctgctttt tgcctcttct tttgccgcat taaccccgga aattggtgcc gtactggcga
     5761 acaccttgag caacctgatt tcagccatat tgctcatcta tctgttccgc ctgtatatgt
     5821 tgattcgcca ataaccttaa gcatttgatt acggaatcgt aaaatgaagc agtttcttga
     5881 ttttttaccg ctggttgtct ttttcgcgtt ttacaaaatt tatgacatct atgcggctac
     5941 tgcggcgctg atcgtcgcca cggcgattgt gcttatctat agctgggttc gctttcgtaa
     6001 ggttgagaag atggccctga tcacttttgt tctggtggtc gtcttcggtg gcttgacgct
     6061 gttcttccac aatgatgagt ttattaaatg gaaggttaca gtcatttatg ctctgtttgc
     6121 gggagccctg ttagtcagcc aatgggtgat gaaaaagccg ctaattcagc ggatgctggg
     6181 taaagaactc acgctgccgc aaccggtatg gtcgaagctg aatctggcct gggctgtttt
     6241 ctttatcctt tgcggtctgg caaacatcta catcgcattc tggctgccgc aaaatatttg
     6301 ggtcaacttt aaagtctttg gcctgaccgc ccttacctta atcttcacat tgttaagcgg
     6361 tatctatatc taccgccaca tgccgcagga agataaatcc taaccagact gccagtcctt
     6421 tggggctggc gctgtatctc ctctgttctc ctcaggaaaa tcatagtagc atcgcgcctg
     6481 tgattttcct tttaagtcgg ttttaccatg tctacaacac ataacgtccc tcagggcgat
     6541 cttgttttac gtactttagc catgcccgcc gataccaatg ccaatggtga catctttggt
     6601 ggttggttaa tgtcacaaat ggatattggc ggcgctattc tggcaaaaga aattgcccac
     6661 ggtcgcgtag tgactgtgcg ggttgaagga atgactttct tacggccggt tgcggtcggc
     6721 gatgtggtgt gctgctatgc acgctgtgtc cagaaaggga cgacatcggt cagcattaat
     6781 attgaagtgt gggtgaaaaa agtagcgtct gaaccaatcg ggcaacgcta taaagcgaca
     6841 gacgcattat ttaagtatgt cgcggttgat cctgaaggaa aacctcgcgc cttacctgtt
     6901 gagtaatagt caaaagcctc cggtcggagg cttttgactt tctgcttact gaatttcggt
     6961 ggtgccgtta attttaaaca ggatattcac cacaatccca ctgcctggct tacccggctc
     7021 ataacgccat ctgcgcatcg catttttcac ctcacgctca aacatgttcg caggcttggc
     7081 tgagaggatt tgtacgttat ccacgcgacc atccggcgtg acgtcaaatt taactttaac
     7141 ctgcccttca atgcgcaatg cctgtgctcg tgccggatac tgcggctgat tacggcttaa
     7201 tgcgcgtggt cctgaagcca cactggtaac cggcttgctg gttgcagccg ttgctgtact
     7261 tgatgtcggg cgtgccggtg ccgtattttc aaacggtgat gccggacgcg actctacggg
     7321 tttgacatcg cgtttttgct gctcctgtac ctttttcacc ggcttcggtt ttggcttagg
     7381 tttcggcttc ggcttttcaa tgaccaccgg tgcttctttt ggcggttcgg ggatcggctc
     7441 aggttccggt tctggctcta ccaccggctc cggtggcggc tgaacggctt gtggcggttc
     7501 gagatcagca ggcgcaacca tcgtgacaga aatcggctgc gcaggcgcag gtagttcaat
     7561 aacctgatgt accgaggtat agagcagacc cgccacaaca gcaccatgaa tgcagaccga
     7621 aagtaacgtc ggccagggga agcggcgagg taaatcaagg gtcattgaag tcataatcat
     7681 ttcagtagaa aaaccaggtc tcgattttaa atgcaaatag caatcatatt caataaggca
     7741 agacgatcag gatcagtgaa aatgtcattt ttggcgtaat tgaccttact ttatgcatgg
     7801 tcttaacaaa gacatcatct ttccgttgca gatcttcgcc ctttcaaata acgtacttta
     7861 caactttccc gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc aagacaaagc
     7921 tgatagcctc gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt tacagttact
     7981 gcatgatgaa ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca gtaacgatcc
     8041 aggggctgcg ggatttactg gctcaaccgt tattgctgaa tttgaatcgc tggaagcagc
     8101 tcaggcctgg gccgatgctg acccgtacgt agcggcagga gtctatgaac acgtttcagt
     8161 gaaaccattt aagaaagtgt tctgagtaga gatccaatat ttaagggcac ggtttttgca
     8221 accgtgttct tgtttggcct ttccttatag gtcggtcatt gcattcacca ccacaagcag
     8281 ataataactt tatctgtatt tctttatatg atgtgagcat gttcaggaaa aatgcccttt
     8341 cctgacgcag agttgctcac tcaaaacctt tgattactgc ggataaagtc atctactatt
     8401 gtttaacaca ttcacgtgtc gcatgtatgt tcagaaaatg tgcacgttaa gataattaaa
     8461 caaaattgtg gttacagagg tatcagtgag tcactgggct acattcaaac aaacagcaac
     8521 aaatttatgg gtgaccctac ggcacgacat tctcgcgctg gccgtctttt taaatgggtt
     8581 gcttattttt aaaacaatct atggtatgtc ggtcaatttg cttgatattt tccatatcaa
     8641 agcattttca gagttggatc tctccttgct ggcaaacgcc ccactattta tgctcggcgt
     8701 ctttcttgtc ctgaactcca ttggcttact gttccgggca aagctcgcat gggcaatcag
     8761 tatcattttg ttgttgatag cgctaattta caccctgcat ttttatccct ggctgaaatt
     8821 tagtattgga ttttgcattt ttacgctggt gtttttactg atactgcgca aagacttctc
     8881 ccacagtagc gccgcagccg ggacaatttt tgcatttatt agtttcacga cattactgtt
     8941 ttactcaacc tacggtgcgc tttatttaag cgaaggtttt aatccgcgaa tagaaagttt
     9001 gatgaccgcg ttctattttt cgatagaaac catgtcaacc gtcggctatg gcgatattgt
     9061 ccctgtttct gaatcagcac gattgttcac tatttcggtc attatttccg gcattaccgt
     9121 ttttgccaca tctatgacct caatttttgg tccgcttatc cgcgggggat tcaacaaact
     9181 tgtaaaagga aacaatcata caatgcatcg taaagatcat tttattgttt gcggacattc
     9241 gattctcgcc attaatacga tcctgcaact gaatcaacgc ggacaaaacg taacggttat
     9301 cagcaacttg cctgaagatg atatcaagca acttgagcaa cgcttaggcg ataacgctga
     9361 tgttatcccc ggtgacagta atgacagttc tgtattaaag aaagcgggaa tcgatcgttg
     9421 ccgggccatt ctggcgctga gtgataacga tgcagataac gcgtttgttg tactctcggc
     9481 aaaagatatg agcagtgatg tcaaaacagt tctcgccgtc agtgatagca aaaacctgaa
     9541 taagattaag atggtacatc cggatatcat tctctcaccg caactgtttg gcagcgaaat
     9601 tctggcgcga gttttaaatg gtgaagagat taataatgat atgctcgttt caatgttgtt
     9661 gaactcaggt catggtattt tcagcgataa cgatgaacaa gaaacgaaag ctgacagtaa
     9721 agaatcggcg caaaaataga gtttaaagac ggaagagtaa acttattggg caccgcaatg
     9781 gtgcccagta catttagata ttgtagatcg caaacaataa cgagttacgc tgtttgttta
     9841 gaatgcactt cctgatgctg tggatacgca tatttcggcg cgactgcccc tcaagccaac
     9901 gcgatttacg tcggctagcc tgacgctgca tgcgccagcg ccccacttcc gttctactcc
     9961 gcttcatgtt aactactcta tgcaataaca gaatggtcat tatacccccg cccccgacgc
    10021 tgaccagtgc ttttcccgcg tttttatttg ccagatgaat ccatatgcgt aaactcataa
    10081 caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt ttatacgttg gtgagttggt
    10141 tggccattct gggatactgg ttgctcattg caggcgtaac tttacgcatt ctaatgaaac
    10201 gacgcgcagt tccctccgcg atggcctggc tgttgattat ttacattctg ccgttagtcg
    10261 gaattattgc ctatcttgcc gttggcgagc tccatttagg caaacgccgc gctgagcgcg
    10321 ccagagcgat gtggccttcc accgcaaaat ggcttaacga ccttaaagcc tgtaagcata
    10381 tctttgccga agaaaatagc agtgtcgctg cgccattatt caagctttgc gagcgtcgtc
    10441 aggggatcgc tggggtcaaa gggaatcagc tacaactgat gaccgagtca gatgatgtga
    10501 tgcaggcgtt aatccgcgac atccagctcg cgcgccataa tattgagatg gtgttttata
    10561 tctggcagcc cggcggcatg gcggaccagg tggctgaatc attaatggcg gctgcgcgac
    10621 gcggcattca ttgccgattg atgctcgact ccgccgggag tgtagctttt ttccacagcc
    10681 cgtggcccga gctaatgcgt aatgccggta ttgaagtggt cgaagcctta aaggtcaatc
    10741 tgatgcgtgt gtttttacgc cgtatggacc tgcgccaaca tcgcaagatg atcatgatcg
    10801 ataattacat cgcgtacacc ggcagcatga atatggtcga ccctcgctac ttcaaacaag
    10861 atgcgggcgt agggcaatgg attgatctga tggcgcgtat ggaaggcccc atcgccaccg
    10921 cgatggggat tatttattcc tgcgactggg agattgaaac agggaaacgt attctaccgc
    10981 caccacctga tgtcaatatt atgccgtttg agcaggccag cggtcatacc attcacacaa
    11041 ttgcttctgg ccccggcttt ccggaagatc tcattcacca ggcattattg actgcggctt
    11101 attcggcgcg tgaatatttg atcatgacca cgccctactt tgtgccaagc gatgatttac
    11161 ttcatgcgat ttgcacggcg gcgcagcgcg gggtggatgt cagtattatc cttccgcgaa
    11221 aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt ctttacggaa ctgcttgctg
    11281 ctggggttaa agtttatcag tttgaaggcg ggttactgca taccaagagc gtgctggtcg
    11341 atggcgaact aagtctggtt ggcaccgtta accttgatat gcgtagtctg tggctgaatt
    11401 tcgagattac cctggcaatc gacgataaag gttttggtgc agaccttgcc gccgttcagg
    11461 acgattatat ttcgcgttca cgtctgctcg atgcccgttt atggctaaaa cgtccattat
    11521 ggcaacgtgt cgccgagcga ctgttttact tcttcagccc gttgctgtaa aacgtcgcca
    11581 acagaggtta aacaggtagt gactatggat atggatctaa acaatcgcct gactgaagat
    11641 gaaacgcttg agcaggccta cgatattttt ctcgaactgg ctgccgacaa tctcgatcca
    11701 gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg gcggcgcgga gttattcgat
    11761 ccggcggaag actggcagga acatgttgat ttcgatttga acccagactt ttttgccgaa
    11821 gttgtgattg gtctggcgga cagtgaagat ggtgaaatca acgatgtttt cgcccgcatt
    11881 ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca tctggcggga ataaataatc
    11941 actttcggcg tgaaaaaggg ctgacagctg tcagcccttt ttgtgtttaa agcggatcga
    12001 ctttcaggca agaaacggcg tgtctgaaac tcccctccag cacaggacgt gttttggcac
    12061 actccggacc ggcaatcggg caacgggtac ggaaaacaca accggaaggc gggttgatcg
    12121 gcgacggtaa ttccccttcc agtaactgga tggttttgtt cttctccaga tccggatcag
    12181 gtatggggac tgccgacatc aatgccctgg tgtaaggatg tagtggattg tggtagacct
    12241 catcataggt ccccagttct accgcatggc cgagatacat caccaacaca cgatcggaaa
    12301 tgtgttttac cacggccagg tcatgagcga taaaaattaa tgacaatccc atctcacgtt
    12361 gcaattgctg gagcaggttg accacctgtg cctgaattga cacgtccagc gccgacaccg
    12421 gctcatcaca gataatcagc ttcggttcaa gaataagagc acgcgcaatc ccgatacgct
    12481 ggcactgccc accagagaac tcatgcggat agcggttaat caggttaggc aataacccga
    12541 ctttcagcat catcgccttc acgcgctcgc gaacttcctg gcgtgacatt ttcggatgat
    12601 aagtacgcag tggttcagcg atgatctcgc cgatggtcat acgcgggttt agcgatgcca
    12661 acggatcctg gaaaatcatc tgaatatcac tgcgaacggc acgccattca tcgggtttca
    12721 tgcccagcaa ctctttacct aaccaggcaa catgaccgtc ggtcgcttta accaggccaa
    12781 tgatggcgcg agcaaaggtg gacttaccgc atcccgattc ccctaccaca cctaatgttt
    12841 ccccttcata caggcgaaga gttacaccat ctacggcttt gagcgttttc ggcggttgcc
    12901 agaaccactg tttgccatct ttgatttcaa agtgcacttt cagatcggcg atttcgagga
    12961 ggacttttct tccttcggtt acagcattca taacagttcc tccaccggtt taaagcaagc
    13021 acgcagacgg ccaggcgtaa actcttccag cggcggtgcg ctactacaaa tttccatcgc
    13081 atgcggacaa cgtggctgga atgggcaacc tttcggtaat cgcagcaggt ttggcggatt
    13141 acccggaata gtcaacattg tttcaccttc cgcatcgaga cgaggcaccg cgttgagcaa
    13201 accgatagaa taaggatgaa cgggttgata aaagacatcg cgcgcgttgc catattccat
    13261 cgtgcgcccg gcgtacatta ccagcacttt gtcgcagatc cccgccacca cgccaagatc
    13321 gtgggtgatc ataatgatgg cggtattaaa ttcccgcttc agttcattca atagcgtcat
    13381 gatctgcgcc tgtacggtga cgtccagcgc agtagtgggt tcatccgcaa tcagcagctt
    13441 aggtcgacat agcaatgcca tcgcaatcat aactcgctga cgcatgccgc cagaaaattc
    13501 gtgcgggtac attttcatgc gtttacgtgc ttccggcatt tttaccgcat cgagcatccg
    13561 caccgactct tcaaacgcct cagctttgct catgttctta tgcagcatca gcacttccat
    13621 caactgctca ccgacgcgca tatagggatt caacgaagtc attgggtcct gaaaaatcat
    13681 tgagatttgt tcagcgcgca gtttattgag ttcacgctct ggcaaattga ggatttcacg
    13741 cccattgaag gttgccgatc cgccaatgcg tccgttggca gccaacaggc ccatcaacgc
    13801 aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca atgcccagcg tttctccggc
    13861 acgtagggaa aaatttaaat cattgaccgc cgtgacgtcg ccgtccgggg tactaaaggt
    13921 gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt tgttgtgcga gcggcacagt
    13981 tgcagtttca attacgctca tggctgcact ccttaacgat ctttcgggtc gagggcatca
    14041 cgcaagccat cgccgataaa gttgaaacaa aacagcgtca ccacgaggaa tcccgctggg
    14101 aacaacagta accatggaga gacttccatt gagttcgcgc catcactcag caatgccccc
    14161 cagctgctta acggctcttg cgtacccaac cccaggaagc taaggaaaga ttcaaagagg
    14221 atcatgctgg gcaccagtag cgatgcgtag accaccacca caccgagtac gttcggcaca
    14281 atgtggcgaa taacaatgcc cgacgtcgat acaccgccaa cttgtgccgc ctcaataaac
    14341 tctttgcgct tcagactcag ggtttgccca cgcacaatac gagccatatc cagccaggaa
    14401 accatgccta tcgccacgaa aatcagcagg atgttttgac cgaaaaaggt caccagcaaa
    14461 atgacgaaga acatgaatgg gaaggagttg aggatttcca gcaaacgcat cattaccgaa
    14521 tccactttac cgcccagata accggaaagc gaaccataaa gtgtccccac gaccactgcc
    14581 accagtgccg cagcaacacc gaccatgagt gagatacgtc cgccaatcgc aacgcgcaca
    14641 agcaggtcgc gaccggatga gtcagtacca aagtagtgac cggactccat atccggggcg
    14701 ctggacatca tcgcccagtc agtatcgtca taggcaaact gcgaaagcat cggtgccagg
    14761 attacaaata acgcgatcag caccagcact atcagacttg caaccgccgc acggttatgc
    14821 ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt cgacctccag cttttcactg
    14881 aaattttcca gcgtctcgct gtttttctta cttaacatca tcgcgagctc cagtatcagt
    14941 aacggatttt cgggtcgata accgcatata gcacatcgac aatggcatta aacaaaatgg
    15001 ttaacgcacc aaccaggatg gtcaggctta acactaagga atagtcacgg ttcaatgcgc
    15061 cattaacgaa cagttgccca atccccggca aaccataaat ggtttcgata accatagaac
    15121 cggtaataat gccgacaaat gcggggccca tataggagag cacgggtaac agagcaggtt
    15181 ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc tttcgcccgg gcagtacgaa
    15241 taaagttgga gtgtaatact tcaatcatag agccacgggt aatacgcgca atactggcga
    15301 tataggcgag tgacaacgcc accatcggca atatcatgaa tttaagcgcc ccaccattcc
    15361 agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat catgactaat aatggcgcaa
    15421 ccacaaaact ggggataaca accccggtca ttgccagccc catcacggta tagtcccatt
    15481 tggtgttttg ttttaatgcg gcaataacgc cagcactaac acccagtatt accgcaagga
    15541 aaaatgctgc ggctcccagt ttggcagaaa cgggaaaact ggatgcaacc aggtcattga
    15601 ccgaataatc tttatattta aacgatggac cgaaatcgcc atgcgccagt tgtttcaggt
    15661 agctgaaata ctgtgtcatg atcggatcat taagatgata tttcgcttcg atattggcca
    15721 ttacttccgg cggtaaagta cgttcgccgg taaaagggct tcccggcgcg aggcgcatca
    15781 taaagaacga aatagtaata agaataaata gcgtcggaat cgcttccaga cagcgacgta
    15841 gaataaattt taacattgcc ctaccttctg gcctgtgcct tctgtaatgc gataaaaatc
    15901 agacaccgtg gagcaggaca ctcctgcccc acgtattgcc atcagtgctt cacaatgtac
    15961 atattccggg tgtaggtatt atccagcgga tctttgccgg tataaccacc aacccacggt
    16021 ttcaccagac gcgcattcac gtagtaataa acaggaacaa tggccgaatc cttatccagc
    16081 tgttgttctg ctttagtgta cagagctgtg cgctgcgcct cgtcagtcac tttcagcgtt
    16141 tccgccataa tgctgtcaaa ggccgggctc ttataatgcg cggtattcat cgagctgttc
    16201 gacagcatgg tgttcaggaa ggaagttggt tcgttgtagt cagcacacca gcctgcacgg
    16261 gccacatcaa aagtaccctg gtgacgggtg tcgaggaacg ttttccactc ctggttaacc
    16321 agtttgacgt ttacaccaat gtttttcttc cacaatgaag aggcagcaat cgccagcttt
    16381 ttatgcagat cggaggtgtt atacaacagg ttgatggtca acggtttgtc tgcggtataa
    16441 cccgcttcag ccagcagttt tttcgcttct tcgttacgtt tttcctggct ccagccaaac
    16501 cattccggct gagtcaattt tgcgccatca gtatacggtg gagtgtaacc ataggcgggc
    16561 atgtcgccct gcgctttcac tttattaaca atgatatcgc ggtccatacc cagtttcagt
    16621 gcggtacgca cacgcacatc gttgaatggc ggtttctggt tgttaatttc gtaataataa
    16681 gtgcacaggt atggatcaac gtgaacttcg tccgggatct cttttttcag cttctggaac
    16741 aattcgatcg gcatgttgtt ataagtcatg tcgatttcac cactacggta gcggttgaca
    16801 tcggtaactt cagaagcaat gggcaaatag gttacctggt taataacggt tttcgcgttg
    16861 ttccagtagg ttgggctgcg ttcaagaacg attcgttcgt ttacgaccca atcttttaag
    16921 gtataggcac cgttggtgac gatattacca ggctgggtcc atttttcgcc gaatttctcg
    16981 atagcggctt ttggcaccgg tgaagttgat gggtgaacaa gtaatttata gaagtacgga
    17041 acgggttcac ttaaggtgac ttctaatgtg tgatcatcaa tagctttcac gccgagatcg
    17101 gtaatcggtt ttttcccttc aagaatttca tcaataccgg cgatatgccc atattgcaga
    17161 taactggcat acggagaagc ggtgttcgga tcaacagaac gttgccagct atacacaaag
    17221 tcttgtgctg tgactggcgt gccatcagac catttcgcat ctttacgcaa atggaaggtc
    17281 cagactttcg cgtctttatt atcccaggat tcagcgacgc caggtgccgg atggccgtca
    17341 agatcgctga ccagtaagcc ttcaaacagg tctcggctga tattagactc cggaacacct
    17401 tcaattttgt gcggatctaa tgactgaact tctgaaccat tgttacgtac cagcgtttgt
    17461 ttttccgcca gtgtgacgcc tgcgggtaca tcagctgcca gcgcgacatt ccctgccatt
    17521 agcgcagcca gaacgccagc tgctactaaa cttctcttgg tgatgttggt cattgttttt
    17581 tggactccct cattataatt actggtatta accagcatgt gtaatcccgg attgggaccc
    17641 tgcacaggct cggagacttt ctgctgtcag gttactttat cgttacggat tgctatcacc
    17701 gactttattt attctggtga ttcagacgat caccttatgt cgattcgtac tgtgtctccc
    17761 ctgtcgagac acattattct ttcgcgacgc tctttaattg ttaattattc tcattaccaa
    17821 taaagaatcg cctgacggtt gcccggaaag taccaaatgc ggttcttact cgccaataca
    17881 ttttgcaaaa ttgttaagca attctctttt attgaccgta tcagaatcta tagcttgcat
    17941 ccatgtctta acaaacatct ttaaattcaa tcaaatagag attggttaag catgtcgatc
    18001 ttttctgatt acagttccag tagtgaaatg cacaataatt taacaattga ttattattta
    18061 gcactttcct ctaccaaagg cagtggaatt actaatatca tttcaattat tcttcagcaa
    18121 gcccaggatt atgatg
//
LOCUS       DABSYT020000088        18066 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.088, whole genome shotgun sequence.
ACCESSION   DABSYT020000088 DABSYT020000000
VERSION     DABSYT020000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.088"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            1021..2166
                     /gene="espG"
                     /locus_tag="ID725_RS18075"
                     /old_locus_tag="pgaptmp_003615"
     CDS             1021..2166
                     /gene="espG"
                     /locus_tag="ID725_RS18075"
                     /old_locus_tag="pgaptmp_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HGV7852127.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAASETYLQNT"
     gene            2294..3112
                     /locus_tag="ID725_RS18080"
                     /old_locus_tag="pgaptmp_003616"
     CDS             2294..3112
                     /locus_tag="ID725_RS18080"
                     /old_locus_tag="pgaptmp_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGV7852128.1"
                     /translation="MKRKPLIAFFIAIFIALTILILFPFNCTYKKSPQEHSLSFIKQL
                     PNTVNLSSLTYNKEDDFLYATQNSPAQLLKITKSGDIMDRAPLPFISDAETIEHIQGN
                     IFAAVDEKTSELFFFTVTKDMHISFRNKIQLEKFNKKNRGFEGLAWKADDRMLFVAKE
                     RRPSKIFIYQLSPDLLSVKQATIPEALNDIRVNDISGLAFNNESLMILSDESRKLLKF
                     NLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLPDESIYVASEPDILAKFVPNK"
     gene            complement(3487..3552)
                     /locus_tag="ID725_RS18085"
                     /old_locus_tag="pgaptmp_003617"
                     /pseudo
     CDS             complement(3487..3552)
                     /locus_tag="ID725_RS18085"
                     /old_locus_tag="pgaptmp_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(3563..3668)
                     /locus_tag="ID725_RS18090"
                     /old_locus_tag="pgaptmp_003618"
                     /pseudo
     CDS             complement(3563..3668)
                     /locus_tag="ID725_RS18090"
                     /old_locus_tag="pgaptmp_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            3848..4021
                     /locus_tag="ID725_RS18095"
                     /old_locus_tag="pgaptmp_003619"
                     /pseudo
     CDS             3848..4021
                     /locus_tag="ID725_RS18095"
                     /old_locus_tag="pgaptmp_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4132..5055
                     /gene="yicL"
                     /locus_tag="ID725_RS18100"
                     /old_locus_tag="pgaptmp_003620"
     CDS             4132..5055
                     /gene="yicL"
                     /locus_tag="ID725_RS18100"
                     /old_locus_tag="pgaptmp_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGV7852129.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5059..5877)
                     /gene="nlpA"
                     /locus_tag="ID725_RS18105"
                     /old_locus_tag="pgaptmp_003621"
     CDS             complement(5059..5877)
                     /gene="nlpA"
                     /locus_tag="ID725_RS18105"
                     /old_locus_tag="pgaptmp_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGV7852130.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            6099..6392
                     /gene="yicS"
                     /locus_tag="ID725_RS18110"
                     /old_locus_tag="pgaptmp_003622"
     CDS             6099..6392
                     /gene="yicS"
                     /locus_tag="ID725_RS18110"
                     /old_locus_tag="pgaptmp_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGV7852131.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(6433..7623)
                     /gene="nepI"
                     /locus_tag="ID725_RS18115"
                     /old_locus_tag="pgaptmp_003623"
     CDS             complement(6433..7623)
                     /gene="nepI"
                     /locus_tag="ID725_RS18115"
                     /old_locus_tag="pgaptmp_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGV7852132.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            7857..8210
                     /locus_tag="ID725_RS18120"
                     /old_locus_tag="pgaptmp_003624"
     CDS             7857..8210
                     /locus_tag="ID725_RS18120"
                     /old_locus_tag="pgaptmp_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGV7852133.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            8194..8517
                     /locus_tag="ID725_RS18125"
                     /old_locus_tag="pgaptmp_003625"
     CDS             8194..8517
                     /locus_tag="ID725_RS18125"
                     /old_locus_tag="pgaptmp_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7852134.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(8633..9085)
                     /gene="yicN"
                     /locus_tag="ID725_RS18130"
                     /old_locus_tag="pgaptmp_003626"
     CDS             complement(8633..9085)
                     /gene="yicN"
                     /locus_tag="ID725_RS18130"
                     /old_locus_tag="pgaptmp_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGV7852135.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(9138..10471)
                     /gene="adeQ"
                     /locus_tag="ID725_RS18135"
                     /old_locus_tag="pgaptmp_003627"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             complement(9138..10471)
                     /gene="adeQ"
                     /locus_tag="ID725_RS18135"
                     /old_locus_tag="pgaptmp_003627"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            10646..12412
                     /gene="adeD"
                     /locus_tag="ID725_RS18140"
                     /old_locus_tag="pgaptmp_003628"
     CDS             10646..12412
                     /gene="adeD"
                     /locus_tag="ID725_RS18140"
                     /old_locus_tag="pgaptmp_003628"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGV7852136.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(12458..13849)
                     /gene="uhpT"
                     /locus_tag="ID725_RS18145"
                     /old_locus_tag="pgaptmp_003629"
     CDS             complement(12458..13849)
                     /gene="uhpT"
                     /locus_tag="ID725_RS18145"
                     /old_locus_tag="pgaptmp_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGV7852137.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(13987..15306)
                     /gene="uhpC"
                     /locus_tag="ID725_RS18150"
                     /old_locus_tag="pgaptmp_003630"
     CDS             complement(13987..15306)
                     /gene="uhpC"
                     /locus_tag="ID725_RS18150"
                     /old_locus_tag="pgaptmp_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGV7852138.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(15316..16818)
                     /gene="uhpB"
                     /locus_tag="ID725_RS18155"
                     /old_locus_tag="pgaptmp_003631"
     CDS             complement(15316..16818)
                     /gene="uhpB"
                     /locus_tag="ID725_RS18155"
                     /old_locus_tag="pgaptmp_003631"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGV7852139.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(16818..17408)
                     /gene="uhpA"
                     /locus_tag="ID725_RS18160"
                     /old_locus_tag="pgaptmp_003632"
     CDS             complement(16818..17408)
                     /gene="uhpA"
                     /locus_tag="ID725_RS18160"
                     /old_locus_tag="pgaptmp_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGV7852140.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(17570..>18066)
                     /locus_tag="ID725_RS18165"
                     /old_locus_tag="pgaptmp_003633"
     CDS             complement(17570..>18066)
                     /locus_tag="ID725_RS18165"
                     /old_locus_tag="pgaptmp_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852141.1"
                     /translation="DSLVQDGIKPEKIAKIISCLSSRTGIRNLFYACSQAFHVANDNA
                     ALDVRRAATSVGDTIDVCPSFVALNGRNYHQHVDFSEYGFTQTLCVLGAVVNNANDTF
                     DVANYIYTTKGENIIHNDAEDDSPYVHSDESYRHKDDYWARDNDIRNLRRNFSGNITI
                     ATYK"
BASE COUNT         5120 a         4577 c         4078 g         4291 t
ORIGIN      
        1 ccatttagtt aagcgtttct ctttataaga tagatctcat tgcataaaca aataaaacag
       61 aataaaaacc aagctgaacc gtttcactat actctactta attcgagtta caggaaggcg
      121 gcaagcgagt gaatccccag gagcttacat aagtaagtga ttggggtaag cgaacgcagt
      181 cgcagcacat gcaacttgaa gtatgacgag tatatatatc tattagctgt ttcccggaca
      241 taagctacag ccagcactaa cctggaacgg gtgcattata aaaaggaaca agggacaaat
      301 agctgaacag taaccgcgtt agtctcttcc cgcataaccg taccattcgt attaagaagt
      361 attgcccata tccattcggc gaaagaggat ctgccataca tcaagtccgt tttacttggc
      421 atcagatcat cgccgtcatt aactctgtta aagtcggaca aacgattagc aagatctgtg
      481 attcaaaagt gcttagcgct aaggtgggac tggttacaga ttcattaacc ctgttctttg
      541 tcgcacctat cgatcggtgt aatgccacaa caggtttatg ttaatcagaa caatgcgccc
      601 ctctataaag agaattcttt gataaggcaa cttgaaaagg gaaagctaac tgatatcaat
      661 gtaccacttt agtaatcatc acattatata caggccaaaa taagtttcct gaaagccagt
      721 atctctaaat caatcattaa tgatatattt tatcgtcaca ataaaaaatt atctaaattc
      781 tattacttat gtaaataagt acaacctttt catagacaca aaaactatgg ctgacgcatc
      841 acttgcaatc aattatccat cattagatta atattttcac ttatttttca gaaggtaaaa
      901 ataactttct atgcgtttaa aacacccgct ataaatatct tgtatattat tgacactata
      961 tgatgctata tgatacttgt tgccaaattg ttcattacaa accagatagg agaatctctc
     1021 atgataaatg gacttaataa tgactccgca tctttagttt tagatgctgc aatgaaagtt
     1081 aattctgggt ttaaaaaaag ctgggatgag atgtcatgcg ctgaaaagtt atttaaagta
     1141 cttagttttg gtttatggaa tccaacgtac agtcgtagtg aaagacaatc atttcaagag
     1201 ttgttaaccg ttttagagcc tgtatatcca cttcccaatg aattaggcag agtatctgct
     1261 cgtttttcag atggttcatc cttaagaatt tccgtcacta acagcgaact tgttgaagcc
     1321 gagattcgca cagcaaataa tgaaaagatt actgtgctcc tggagtcaaa cgaacaaaat
     1381 aggttattac aatctttacc catcgatcgc cacatgccat acattcaggt tcatcgtgcc
     1441 ttatctgaga tggacctgac tgatactacc tcaatgcgca atctacttgg ttttacgtca
     1501 aaactatcaa caaccttgat tcctcataat gctcaaacag atccgctttc cgggcctaca
     1561 ccattcagct ctatctttat ggatacatgt cgaggactcg gcaatgcaaa gctttcactc
     1621 aatggtgttg atatacctgc aaatgcacaa aaattgcttc gcgatgcact aggacttaaa
     1681 gacacacatt catcaccaac ccggaatgtt atagatcatg gtatttctcg ccatgatgca
     1741 gagcaaatag caagagaaag cagcggcagt gataaacaga aagctgaagt tgtggaattt
     1801 ttatgccatc cagaagcagc aacggccata tgctcggctt tctatcaatc tttcaatgtg
     1861 ccagccttaa cgttgacaca tgaaaggatc tctaaagcca gtgaatacaa tgcggaaaga
     1921 tcattagata cacctaacgc ttgcattaac atcagtatct ctcaatcatc agatggaaac
     1981 atttatgtta ccagccatac tggggttctg ataatggcgc cagaagaccg ccccaacgag
     2041 atgggcatgt tgacgaacag gacttcttat gaagtgccgc aaggtgtgaa atgtataatc
     2101 gatgaaatgg taagtgcgct acaaccaagg tatgccgcat ctgaaacgta cttacaaaac
     2161 acttaaaata aaactttatt aatcaaaact aataatagaa accacaccca taattattaa
     2221 caatttattc cgaagttaat cacaattaaa caaagatatt tattctgcaa acaatttaca
     2281 gcaaatagag aaaatgaaaa gaaaaccatt gattgcattt ttcatcgcga tatttattgc
     2341 actaaccata ttaatattat ttccatttaa ttgtacttac aaaaaatcac ctcaagaaca
     2401 ctcactttca tttataaaac aattaccaaa tacggtgaac ttatcatctc ttacttataa
     2461 taaagaggat gatttcctgt atgccaccca gaatagccct gcacagctac ttaaaataac
     2521 caaaagtggc gacatcatgg acagagcccc actgcctttt atctctgatg ccgaaacaat
     2581 tgaacacata caagggaaca tttttgctgc agtagacgaa aagacttctg aactattctt
     2641 ttttacagtt acaaaagata tgcatatctc ctttcgaaat aaaattcagt tagaaaaatt
     2701 caataaaaag aatagagggt ttgaagggct ggcatggaaa gcagatgatc ggatgctgtt
     2761 tgtcgcaaaa gaaagacgcc caagtaaaat atttatatat caactttctc cagacctgct
     2821 gagtgtaaag caagcaacta ttccagaggc actaaatgat atacgtgtta acgatatatc
     2881 cggcctggcc tttaataatg aaagtctgat gattctgtcc gatgaatcgc gtaaattact
     2941 caagtttaac ctgacagaga tgtctttcat tgagatgctt gacctgacaa aaggaaatca
     3001 ttctctgacc agtgatttgc cccaacctga aggaattgta actctgcctg atgaaagcat
     3061 ttatgttgcc agtgaacctg atatcctcgc gaaatttgtc cctaataagt aagtctgtca
     3121 ctggccttat tatttatcga ccaaacctgg caatctcata aagttataaa aatacgagta
     3181 taatttcacg ctaaataaac acattaatag ttgaaaagga tatcagttat tatcaaccag
     3241 aatcttactc agtctattag tattatcaga cttatattag atcctacaat atatttaatt
     3301 tgtatgcaga atagagtgtt cacaccacat ctggtataga aaagcacagg ctagcaaaaa
     3361 cctgaataac cccgtactta cggagttatt caggttaaaa atatccataa catcacttct
     3421 gatttttatt gcttacgacg gatataattt ctctggtcag ctactaatat catcatgctc
     3481 acgcgtttaa tcaaactcgt gtttctcaaa cggacatcgc gggacaatct tctgcttctc
     3541 ccggcgcatt ttttaccacc ttcgctacct gcattttgag cggaagatcc gcttttttct
     3601 ggcccccgca catatgcaag tccagtttta aatttatcct ttatttcatt ctgaaacaaa
     3661 ctgctcataa ttttctccag tggaacctgc atctcaggga gacatcacta cagaatactt
     3721 ccccttatgc actgatttaa ttttggtcat ttagaataac ggcctgtaaa gtctcaatgc
     3781 aacagatggg taacgctatt atgaggatcc tcgtcagtaa attactggca aggcactccg
     3841 ataaataaga ataggttccg caacaacgtt atatgcaaat acctcacgct tcggctagat
     3901 tatcgccggc tctgttgacg aaattatggt tgaaatctgg agctaccaca cgttgttctg
     3961 gctggcgata gggatgttgg gtattgcaat gatttgcttg ctgtttatta aagatattta
     4021 gctcaccagt ctgggctaag aaaaatcggc gtggcgcaaa ctgagccatc ctcgccattt
     4081 ttgctatcat gcctgcatac ataaacgaca aaacagtatg cagagggaaa aatgggttcc
     4141 accagaaagg ggatgctgaa cgttctgatt gccgccgtgt tgtggggaag ttcaggggtc
     4201 tgcgcgcaat acatcatgga gcaaagccag atgtcgtcgc agtttttgac tatgacgcgt
     4261 ttgatattcg ccggtttgat tctgctgacg ctatcatttg ttcatggtga taaaatcttt
     4321 tctattatta acaatcacaa agatgccatt agcctactga ttttttccgt ggttggcgcg
     4381 ctaaccgtac agctcacttt tttgctaacc atcgaaaaat cgaacgcagc cacggcaacg
     4441 gtgctgcaat tcctctcacc gactattatc gtcgcctggt tctcactggt gcgtaaatcc
     4501 cgcccgggca ttctggtttt ctgcgctatt ttgacatcgc tgatcgggac ttttttattg
     4561 gtgacacacg gtaatccgac gtcattatcg atctctcctg ccgcgttatt ctggggcatt
     4621 gcctcggcat ttactgctgc attctatacc acctatccct caacgctgat tgcccgctat
     4681 ggcacgttac cggtcgtcgg ctggagtatg ctcattggcg gtctgatcct gctgcccttt
     4741 tatgccggac aagggacaaa ctttatcgtt aacggaagtt tgattctggc gtttttttat
     4801 ttggtggtca ttggtacgtc cctgacattt agtctgtacc tgaaaggagc acaattaatt
     4861 ggcggtccaa aagccagcat tttaagctgt gctgaaccat taagtagcgc gctgctctct
     4921 ttgctgttgc tggggatcac cttcacattg ccggactggc tgggaacgct gctgattctg
     4981 tcatcggtga ttttgatttc aatggattcc cgccgccgcg ccagaaaaat aaatcgtccg
     5041 gcgcggcatg agtgagaatt accagccagg caccgcacca ccgttaaaga tggtttctgc
     5101 cgctttagcg acttcgggtg attgataaga ttgcagaaat tccttcacgt tttctgcatt
     5161 cttattatct tcccgtgcca ccagaatatt cacatacggc gaatttttat cttcaataaa
     5221 tacgctgtcg ttaaccggag aaagtcctgt ttgctcgatg taagtggtgc tgataatcgc
     5281 tacatccact ttcgggtcgt ccagtacacg tggcaactgt gccccttcca gctccattat
     5341 ctgcaaatgg cgtggattat cggttatatc caacgcggta ggtaataacc cttttccctc
     5401 tttcagagta attagcttct ctttttgcag cagtaaaagc gcgcgaccaa ggttagtcgg
     5461 gtcgttgggg atcgccactg tcgcaccttc ttttatttgc gccacggttt tgattttttt
     5521 ggaatagccc gccataggga agacaaacgt atttcccacc gctaccagtt tataaccatg
     5581 cgtctgatta tcttgttcaa gaaagggtcg atgctggaag acgttcgcat caagctcgcc
     5641 attatttgtc gcatcgttcg gtagcaacga accgctaaat cctaccagtt caacatcgag
     5701 gccatatttc tcttttgcta cctttttagc gacttctgcg acatcttgtt ctgcgccatt
     5761 aataacgcca actttaatat gcttttcatc gctgctgctc tggtcgcaac ctgccagcag
     5821 aactcctgcc agtaataatg cggcccccgc ccgtagatga tgtgttgtca gtttcatttt
     5881 tttatccttt tattaatgat taccggacta tagcggtaag gtcgctgcag tttaaaaaac
     5941 aaaacgctat cgataagaat aaaaaggaat aaaagtggaa tataaagtct ggcacttctt
     6001 actgaccacg caaacacctt tcgtacagca tgatgagagc ggcaaaagca aactacactt
     6061 atgcttcatt cgatgcacat ttgcagaagg ttgccgctat gaagccaacg atgctactca
     6121 tgattaccgt attcctgatt tttccagccc tttctcaggc cgagtcgcca ttcagttctc
     6181 tacagtcggc aaaagagaaa accacggtat tgcaagattt acgtaaaatt tgcacgccac
     6241 aggcgtcatt atcagatgaa gcgtgggaaa agttaatgtt gtcagatgaa aacaataaac
     6301 aacatatacg cgaggccatc gtggcgatgg aacgtaataa tcagagcaat tattgggaag
     6361 cgttgggtaa ggtagaatgc ccggatatgt aagaacggca acaaccactg accaacgctt
     6421 ctttacctga tatcaggatt tcttcatttt cacctttgca gtcaccaaca atgcagtcag
     6481 caacatcaat gtgccggaca acatcagcgg cgaagtcaga ccaatgttat ccaacgcata
     6541 accgccgatt gccgcaccgc aggtattggc aagctgaata acggcaacct gaatagatcc
     6601 agccttttct gcctgatcgg ccaatgagcg agtgatccac gttgaccaac caacgggaat
     6661 taaagcaaag gtcagccccc agataatcgc cacaccagta gcaacaattt tatcgcttcc
     6721 ccacagcgtc agtaccagcg cgctcagggc gagcacaaac ggtgcgcccg ctaaggccag
     6781 tttcaccgaa cgtttgagaa taaacgacga aagcgatgtc ccgacaaagc tggcgatacc
     6841 aaaactcagc agcaccaggg ttaagccatc aacaccgaat cccgccagag tcatatacac
     6901 tgggcgaata taagtgaaaa aagcaaactg tccggcaaag gacataaaga tggcgatcat
     6961 cccggccatc actcccggac gttgtaataa gcggaaagta ttttgtttct gatgcgaagg
     7021 ttcacctggc agcgaaggca gtgatttgat aatccagaaa atacacagca cacccatcgc
     7081 cgccgccgca ttaaagacat tgcgccaacc gataagctcg cctaaaaaac agcccaacgg
     7141 cgcggcaatc accagcgcaa tagaaaccgc gccgaagatc accgacagcg ccttcggcac
     7201 cgtacgcggc ggcaccagac gcatggtcag cgacgccgac atcgcccaga acccgcccag
     7261 cgccagcccc agacaggcac gaccgattaa aagcaaactg aatgagttag caaaggaaac
     7321 cagcaagcag gagagcgtca gcaaaacggc aaacaaaata acaacgtagc ggcggtcagt
     7381 ggcctgaatt gtctgggtaa taaacaaact ggcaaacatt gccacaaagg cggtcacggt
     7441 caccgattgc ccggcaaccc cttccgaaat gcctaaatcc tgggccattg gcgtcaacaa
     7501 actgacaggc aaaaactcaa cgataatcag acaggcgacg caaaacgcca ccgagaaaac
     7561 ggctgaccag ttcggtcggg tgatggcatc cgcgccgcgg ttttcggcaa taaattcact
     7621 catggtgtta cccgtggcat gtttcagggg aaaagccgac cactttaaca ttgaaatgtg
     7681 tgacgcattt aacgtttttg caacttttac ggtgttgtgg gaaaagctcg aggctgctcg
     7741 aagaaataaa aatcatagga caaggtgata tgtattttcg cgattgtgtt gggaacaagg
     7801 gataaagtat tgcacttggt gatatacttt aataacagga cctcaacgat atacgcgtgt
     7861 ttaaaaccaa atggtttgca agggaagcca ggtctcatgc aataacagat gaagagttgt
     7921 gccgggccat tttagaaaca gaacaaggta aagctgatgc acttggcggt ggcgttttta
     7981 aaaagcgcct tcaccaaaat cgtgagcgtg caattattct ggcgaaaggg gtaagcaatt
     8041 ggttttacac gtttttatat gcaaaacagg atatgtcgaa catcaatagt caggaactgg
     8101 ctgggtttcg tgaaattgca aagcattatg ccttcctgac gaaagcccaa ctaacagcaa
     8161 tgattaacac taaagaactg acagagatct gctatgactg caaaaactaa atttaaaagt
     8221 cctgcatttg aggctattca cagcgcagcg gctggattgt ccagcgtcaa tgctatttct
     8281 gctgaaacga tgcgtacttt tgacaaagcc tgcctgacca gcgtgcaaga cttacaacct
     8341 gttgagataa aagcactgcg ggaaaaatta aaagtgagtc agcccgtctt cgctcactat
     8401 ctcaatacca gcgtttcaac tgtacaaaaa tgggaaagtg gcgctaaacg ccccaatggc
     8461 atttcattaa aactgctcag cattgtgcaa aaacacgggt tggaggtgct gctgtaaata
     8521 gaacgtagat aatgcgttca cctactgtca gttccgatac cggatgcggc gtgaacgcct
     8581 tatccaccct acaggtcagg cgcggtctgt aggctgataa gacgaagcgg ttttaatgca
     8641 gccagtgaac gccctcggca ggctgaaaga acgcgttata gcgcatctga aaagcattaa
     8701 tatcctgcat ttcaggttcg gtttcgcgca gaaaacccag cgccagcctg acctgagcat
     8761 cagtaatggg agcggcaagg cgggcttttt gtaaaatccg atgccattgc agcaggttct
     8821 gttcgctacc atcgacaatg acaatttgcc agatgagcaa cacctgcgcg gcgttttgca
     8881 ggtgcgactc atccggacga tgcaaataac gtaaaaattc gtcaccggct gactttccca
     8941 tttgatcgac catcgactcc acgggtacga catcgatatt cagccgatcg ctgagacggc
     9001 gaatcgcttt tctggccccg gatgtcagca cccgaaaacc aaccacaaac actaccgcca
     9061 gcgtggcgag cattatccag atcatgagtt ctcctgcgta atcagaactc atgatatctg
     9121 gaaaccctcg ccagttacta atccaccaga ataatcttca gtgcaaacag agctgcgacc
     9181 accaccacac acaggttcag atcgcgccag cgcccggtgc acactttcat gatgcagtac
     9241 gacataaagc caagtgcaat cccttcggtg atcgagaaag taaagggcat catcaccgtg
     9301 gtaacaaacg caggcaccga ttcggtaaaa tcatcccagt taacgcgcgc cagacttgag
     9361 gtcatcaaca cgccaacaaa gattaacgct ccggcggttg cgtaaggagg aaccatcgcc
     9421 accagcggtg agaagaacat aaccaacagg aacataacgc cgaccacaac cgcagtcagc
     9481 cccgtgcgac caccgactgc cacaccagaa gtactttcaa tataggcggt aacagacgag
     9541 gtgccgataa acgcgcccgc caccgaacta cgctatcaac atatagcgcc ttattcatat
     9601 tggggaattt accgttacta tctattaagc cagctttatc agttacacca attaatgttc
     9661 ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg agttcaagtg
     9721 ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc ggaatggaat
     9781 agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc accacaatag
     9841 aaacaagcac cgcggcatga aaatgacgtg atgacaacac ggttataata aaaaacccta
     9901 aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccaga gtgtctttat
     9961 tggcgacaat aacgccagta ttttttaatc ccattaaggc gataaataat ccgattccgc
    10021 tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac cggatacgaa
    10081 atagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc ccggtctgcc
    10141 aggagatacc catcgccccc acgaccacga acgcaaagaa ggcgttcagc cccattgccg
    10201 gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg ataccggcaa
    10261 tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg aggatttgcg
    10321 ggttcacaaa aacgatgtac accatggtta aaaaagtggt catccccgca atcaattctg
    10381 tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc cctgattcat
    10441 tactcacgta atcggtattg tcattattca tttttttgtc cattggagga gatttaatcc
    10501 cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca tttcaattgc
    10561 acattaatgg atattcttta ataatcttgc gacatttctt tatgataaat aacaatcaaa
    10621 ttaataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat ttcatcacat
    10681 tagccgggcg gaataccagg aattgttagc cgtttcccgt ggcgacgctg ttgccgatta
    10741 tattattgat aatgtctcta ttctcgacct gattaatggc ggagaaattt ccggcccaat
    10801 tgtgattaaa gggcgttaca ttgccggtgt tggcgcagaa tacactgatg ctccggcttt
    10861 gcagcggatt gatgcccacg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca
    10921 tattgaatcc agcatgatga cgccggtcac ctttgaaacc gccaccctgc ctcgcggcct
    10981 gacaactgtt atttgcgatc ctcatgaaat cgtcaacgtg atgggcgaag ccggtttcgc
    11041 ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg tcagctcttg
    11101 cgtacccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct
    11161 cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt
    11221 aattagcggg cagaatgcgc tgctcgataa actggatgca tttcgccacc tgacgctgga
    11281 cggtcactgc ccgggtttgg gtggtaaaga acttaacgcc tatattgctg cgggtattga
    11341 aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac tcggcatgtc
    11401 gttgatgatc cgcgaagggt ccgctgcccg caatctcaac gcactggcac cgttgatcaa
    11461 cgaatttaac agcccgcaat gcatgctctg tactgatgac cgtaacccgt gggagatcgc
    11521 ccatgaagga cacatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct
    11581 gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc tgaatcacct
    11641 cggcttactg gcacctggta agcaggctga tatcgtcctg ttgagcgatg cgcgtaaggt
    11701 cacggtgcag caggtactgg tgaaaggcga accgattgac gcgcaaacct tacaggcgga
    11761 agagtcggcg aaactggcac aatccgctcc gccatatggc aacaccattg cccgccagcc
    11821 agtttccgcc agcgactttg ccctgcaatt tacgcccgga aaacgctatc gggtcattga
    11881 cgtcatccat aacgaattga ttacgcactc ccactccagc gtctacagcg aaaatggttt
    11941 tgagcgcaat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac gtctggctcc
    12001 ggcttgtggt ttgctcggcg gctttggtct gaatgaaggc gcgctggctg cgacggtcag
    12061 ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg cgctagcggt
    12121 caatcaggtg attcaggatg gcggcgggtt gtgcgtggta cgtaacggcc aggtccaaag
    12181 ccatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt cgctggcgga
    12241 gcaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg atgagccgtt
    12301 tattcagatg gcgtttcttt ccctgccagt gatccccgcg ctgaaactaa ccagccaggg
    12361 gctgtttgat ggcgagaagt ttgccttcac cacgctggaa gtcactgaat aataaaaaaa
    12421 gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc aactgctgaa
    12481 tttttttctc gcggcggatt ttgcgttctt ccatcaccgc cactatcgcc atcaggcaga
    12541 tacaaccaat cgcggcgata tccagcgcag cgaaggtgcc tgcccagccg gtaaggccga
    12601 atactggcgt cccatcggca atcattccca gacctaactt ggcaaagctg tcaccaatca
    12661 ggtaagcgaa ggtgccttta ataccatcgg cagcgccaat cgctttttta ggtacaaagc
    12721 caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc aacgcaaaga
    12781 gagaagccag atagatatat tcgttgctgg catgttggta cacaccgagc gtggcgataa
    12841 tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgctagg tcagagagcc
    12901 agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg
    12961 ccaccgcttt agagagtttc agttcctgga acgcgtacac agttgaccac tggtcgatac
    13021 caatacgcac cacatagagg aaaatgttgg cgaagcacag cagccagatc actttgtttt
    13081 tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct gtctctttgt
    13141 cctcttcgct gatctcctcg ccgaacagct cttcagcttt gccgaggcca taagattccg
    13201 gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata atcgacggga
    13261 agataaacat gccgatgaca tggccatcga acaggtaatt tgcgccgaac agtgccacac
    13321 cagctgcgcc tgcaccgcca aggttgtgag aaatgttcca gaaaccgagg aacgtcccgc
    13381 gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccc ccggtactct
    13441 ggaaaaagcc acttaaggcg tagaaggcaa tcatcaggaa caggctaacc gagccgctgc
    13501 ccatactggc gctaaagccc agcatacaaa tggcagacag gatcagcatg aacggcagga
    13561 attgtttggt gtttttgccg tcggcgtagt aggaaaccag cgttttaccc acgccataag
    13621 tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg taggtcgaaa
    13681 tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc aggtagccga
    13741 taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc cgcacttcga
    13801 gcggaaggtc cagggtcggc ttgcggacct ggtttaagaa agccagcatg ggttactcct
    13861 gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca ggcctgagat
    13921 agcgtccagg caaaacctgg aaaatttctt agttttgccg gatatgaagt gaaaaggtga
    13981 gatgcatcac gcttcgcgcg gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga
    14041 aatcccggcg gcaatggcga taaccacaaa aaatccgctc cagtgccagg tatcgagcac
    14101 tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc cgacaaaccc
    14161 cgtcgccgcc cctgccgcct ctttgtggga acattctgcc gccgccatgc cgattaacat
    14221 ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca tcacgtagct
    14281 ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcag cgaaaatcaa
    14341 attcatcggc ccgcggttgc cgttaaacag tttgtccgag ccccaacccg ctaccagcgc
    14401 accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga ccagatcgac
    14461 gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc gaaccacata
    14521 gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcaacaaca catacttggt
    14581 gaggatctct ttacgcgtca gccctgcccc ttcttgttgt tgagcaatct ccagcgcgtc
    14641 gtgtcgccat tcaccgaccg ctggtaaacc taacgcctgc gggcgatcgc gcagccgcca
    14701 gcagagaaaa atccccacga cgatcgccat acaaccagca atcatcatcc cggcacgcca
    14761 gccgtaatgc agcgcagctg ctgccatcac aatgggaatg agcgcaccgc caacgttatg
    14821 cgccgtgttc cataatgccc accaaccacc gcgttcggta cgagaatacc aggccgttaa
    14881 cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca cccagagcac
    14941 ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttgatgatgc ccgtggcgat
    15001 aagccctatc cccataaaat aacgggcatt tgagcggtcg ctgacaatgc cggagacaaa
    15061 cttcgacacg ccgtaggtaa tgtaaaacag ggtcgctaac aggccgatat cgctacggct
    15121 gagcacgcca ttagcaagga tttctggtac ggcggcgtta aaacttttac gcgtgaagta
    15181 aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt agcgataacg
    15241 ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg
    15301 caacatcatc aaaccttaga catagcgttg aggtagagaa acgctgacac gcgtgccgtg
    15361 cagacaggaa atggtcaagg tgccgcccag cgccgttacg cgctcgcgca ttccggtgag
    15421 gccaaaacct tgttgcccgg aacccggcgg taaaccgctg ccatcgtctt caatcaccag
    15481 catcaaccgt tcatcctgca gccagccttg cagggtgacc gcgctggcat cagcatgttt
    15541 cacaatgtta ttcagccctt cctggcagac acgaaacagc gtcacgcgct ggttttcgct
    15601 taacgctgac tcttcgattc gccattcgag atggctgaca atgccgcgcc cttccagctc
    15661 catttcccgc atcagtgagc ggatggcctg ctctagggtg agatcatcca actggcgcgg
    15721 acgtaaccgc cctaacaaac ggcgcaccgc gtcgtacacg cccagcgaca gttgttcgat
    15781 gagctgcccg ctctgcttca cactggcgtt atctgccgcc agtcgctgga caatgcccgc
    15841 ttgagtacga atggcagtga tggtctggcc gatatcatca tgcagctcac gcgccacatc
    15901 acggcgcacg ctctcttcag tttctaacaa acgttcagcg agatgctgat tgcgcgccag
    15961 ttccttttgc agcgactggt taagttcacg caaccgctgg atgccagcgc ccagtaacaa
    16021 ccctgtcaga ctttgcacca gcagcgagag caataaatcc acaggatgat cgcgccaggt
    16081 ttgactggcg atcagcgcga tggcgttcat caatgtcgct atcagcgccc cttgccagcc
    16141 gtagtgccag gccagcgcga taatcggcag cgccaggcag aatggcgtaa agcgcgacag
    16201 ttcgtccggc aagcccaact ggagccagag actgataaca aacagcagca agtaccagac
    16261 cagatgccgc ccgcgccagt tgatcggttg agaaactaac gacggcccga gcggcagcca
    16321 ggtgttattg gcgagataat gccagaacac cagacatatc ggggccagcg tcaggccgcc
    16381 agttaaagtt aacagcagcg cattccacga ctctttgccg tgccaaagcc agggcagcga
    16441 ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc gccagtcacg
    16501 ttgatggcga tagcgcgaga tcagcgccac gggcagtaac gtcagtaaac taccgatcat
    16561 caataaggag aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca gcagccactc
    16621 cgctcccagc agcaccggcc agtatccgcg cgggcattgc agcattagcc ctagccgcag
    16681 accaaacgga aataacagca ccgccatatc agggcgctca accaggtgca ggctgatact
    16741 ccacaggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg taattaagcg
    16801 ggagaacaac gtcttcatca ccagccatca aacatacggc gcgccagttc aacgtcgtta
    16861 ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt cggtgacaag
    16921 cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc cgccacctgc
    16981 cgttcacgtt tggttagtgg gtcctggcga ccggatgcca gtttaatggc aatatccggc
    17041 gtcagataac agccgcctgt ggcaaccgta tgcaccgcag caatcagttc gtcagggcta
    17101 caacgcttgg agagaaagcc acgcgccccc gcgttaagcg cctgctcaac cagcgccgga
    17161 ctgtcatgaa cggagagcat aattgtcgcc atacctttcg gcagctggct tagcagctcc
    17221 agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc gcgccccggc
    17281 agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacttgcaa atcaggttcc
    17341 agccccagca actgcgcaaa gccggagcgg acgatgaggt gatcgtctat aagggcaacg
    17401 gtgatcatga ttgggtcctg gcggataaaa aacgcgcata ccttaacgat aagcgcgatg
    17461 ttgttcaagt tggatatggg gtatgtattg gcttaatgat gttgagctta tcgtcaaaac
    17521 gcttacctga gaggtaagcg tttggtgcgt gttattgaga aaaagcgaat tatttgtagg
    17581 ttgcaatcgt aatattgcct gagaaatttc tccttagatt gcggatatca ttatcccttg
    17641 cccaataatc atctttgtgt ctataactct catcggaatg tacataggga gaatcatctt
    17701 cagcgtcgtt atggataata ttttcgcctt tagtggtata gatataatta gcaacatcaa
    17761 aggtgtcatt cgcgttattt acgactgcgc ctaagacgca aagggtttga gtgaatccgt
    17821 attctgagaa atcgacatgc tgatggtaat ttctaccatt caaagcaaca aaactcggac
    17881 aaacatctat tgtatcgcca acagacgtag cggcgcgacg cacatcaagt gcagcgttat
    17941 cgttagcaac atgaaatgct tgtgaacagg cataaaacaa atttctaatc ccggtacgac
    18001 tggataaaca agaaataatt tttgctattt tttctggctt tataccatcc tgtactaaag
    18061 aatctc
//
LOCUS       DABSYT020000089        17950 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.089, whole genome shotgun sequence.
ACCESSION   DABSYT020000089 DABSYT020000000
VERSION     DABSYT020000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.089"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(393..1589)
                     /gene="hemY"
                     /locus_tag="ID725_RS18170"
                     /old_locus_tag="pgaptmp_003634"
     CDS             complement(393..1589)
                     /gene="hemY"
                     /locus_tag="ID725_RS18170"
                     /old_locus_tag="pgaptmp_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGV7852142.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1592..2827)
                     /gene="hemX"
                     /locus_tag="ID725_RS18175"
                     /old_locus_tag="pgaptmp_003635"
     CDS             complement(1592..2827)
                     /gene="hemX"
                     /locus_tag="ID725_RS18175"
                     /old_locus_tag="pgaptmp_003635"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGV7852143.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQADTPAAAPQGE"
     gene            complement(2849..3589)
                     /gene="hemD"
                     /locus_tag="ID725_RS18180"
                     /old_locus_tag="pgaptmp_003636"
     CDS             complement(2849..3589)
                     /gene="hemD"
                     /locus_tag="ID725_RS18180"
                     /old_locus_tag="pgaptmp_003636"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGV7852144.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3586..4527)
                     /gene="hemC"
                     /locus_tag="ID725_RS18185"
                     /old_locus_tag="pgaptmp_003637"
     CDS             complement(3586..4527)
                     /gene="hemC"
                     /locus_tag="ID725_RS18185"
                     /old_locus_tag="pgaptmp_003637"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGV7852145.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4914..7460
                     /gene="cyaA"
                     /locus_tag="ID725_RS18190"
                     /old_locus_tag="pgaptmp_003638"
     CDS             4914..7460
                     /gene="cyaA"
                     /locus_tag="ID725_RS18190"
                     /old_locus_tag="pgaptmp_003638"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGV7852146.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7500..7820)
                     /gene="cyaY"
                     /locus_tag="ID725_RS18195"
                     /old_locus_tag="pgaptmp_003639"
     CDS             complement(7500..7820)
                     /gene="cyaY"
                     /locus_tag="ID725_RS18195"
                     /old_locus_tag="pgaptmp_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGV7852147.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(7982..8124)
                     /locus_tag="ID725_RS18200"
                     /old_locus_tag="pgaptmp_003640"
                     /pseudo
     CDS             complement(7982..8124)
                     /locus_tag="ID725_RS18200"
                     /old_locus_tag="pgaptmp_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8283..8486
                     /gene="yifL"
                     /locus_tag="ID725_RS18205"
                     /old_locus_tag="pgaptmp_003641"
     CDS             8283..8486
                     /gene="yifL"
                     /locus_tag="ID725_RS18205"
                     /old_locus_tag="pgaptmp_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGV7852148.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8523..9347
                     /gene="dapF"
                     /locus_tag="ID725_RS18210"
                     /old_locus_tag="pgaptmp_003642"
     CDS             8523..9347
                     /gene="dapF"
                     /locus_tag="ID725_RS18210"
                     /old_locus_tag="pgaptmp_003642"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGV7852149.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9344..10051
                     /gene="yigA"
                     /locus_tag="ID725_RS18215"
                     /old_locus_tag="pgaptmp_003643"
     CDS             9344..10051
                     /gene="yigA"
                     /locus_tag="ID725_RS18215"
                     /old_locus_tag="pgaptmp_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HGV7852150.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQALANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10048..10944
                     /gene="xerC"
                     /locus_tag="ID725_RS18220"
                     /old_locus_tag="pgaptmp_003644"
     CDS             10048..10944
                     /gene="xerC"
                     /locus_tag="ID725_RS18220"
                     /old_locus_tag="pgaptmp_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGV7852151.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10944..11660
                     /gene="yigB"
                     /locus_tag="ID725_RS18225"
                     /old_locus_tag="pgaptmp_003645"
     CDS             10944..11660
                     /gene="yigB"
                     /locus_tag="ID725_RS18225"
                     /old_locus_tag="pgaptmp_003645"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGV7852152.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11744..13906
                     /gene="uvrD"
                     /locus_tag="ID725_RS18230"
                     /old_locus_tag="pgaptmp_003646"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11744..13906
                     /gene="uvrD"
                     /locus_tag="ID725_RS18230"
                     /old_locus_tag="pgaptmp_003646"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGV7852153.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13951..14853)
                     /locus_tag="ID725_RS18235"
                     /old_locus_tag="pgaptmp_003647"
     CDS             complement(13951..14853)
                     /locus_tag="ID725_RS18235"
                     /old_locus_tag="pgaptmp_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HGV7852154.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(14936..15700)
                     /gene="yigE"
                     /locus_tag="ID725_RS18240"
                     /old_locus_tag="pgaptmp_003648"
     CDS             complement(14936..15700)
                     /gene="yigE"
                     /locus_tag="ID725_RS18240"
                     /old_locus_tag="pgaptmp_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGV7852155.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15862..15918
                     /gene="ysgD"
                     /locus_tag="ID725_RS18245"
                     /old_locus_tag="pgaptmp_003649"
     CDS             15862..15918
                     /gene="ysgD"
                     /locus_tag="ID725_RS18245"
                     /old_locus_tag="pgaptmp_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGV7852156.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16070..17020
                     /gene="corA"
                     /locus_tag="ID725_RS18250"
                     /old_locus_tag="pgaptmp_003650"
     CDS             16070..17020
                     /gene="corA"
                     /locus_tag="ID725_RS18250"
                     /old_locus_tag="pgaptmp_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGV7852157.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17062..17553)
                     /locus_tag="ID725_RS18255"
                     /old_locus_tag="pgaptmp_003651"
     CDS             complement(17062..17553)
                     /locus_tag="ID725_RS18255"
                     /old_locus_tag="pgaptmp_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HGV7852158.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCITKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIEQVFNPHNFKK
                     DNF"
     gene            complement(17550..>17950)
                     /locus_tag="ID725_RS18260"
                     /old_locus_tag="pgaptmp_003652"
     CDS             complement(17550..>17950)
                     /locus_tag="ID725_RS18260"
                     /old_locus_tag="pgaptmp_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HGV7852159.1"
                     /translation="IKECIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSF
                     NGLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFR
                     IWFFLQRHKDFAFKPFFTNFNTIERIENYL"
BASE COUNT         4154 a         4606 c         4732 g         4458 t
ORIGIN      
        1 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
       61 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      121 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      181 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      241 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      301 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgtg tcaggaaggt
      361 cccggaggtg cttgcctccg ggtgagaagg aactactgtg gtgggttgtt ctgcaacgtt
      421 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      481 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc tgcgcggaag
      541 gcgagcgatg cttcctgcca ttctccgtgc ttcatcagtg actggcccag tgtgctccac
      601 aacagcgggc gatcgccgac gtttttgatt tgctggcgca gcactttttc cagctgttcc
      661 ggattgtttg ttttcagtcg aggaatcggc agcagtaggc gatcgtcgta ctggcgtttc
      721 aggccatcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
      781 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
      841 ttacgcaaac cttcgctacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
      901 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
      961 ataatatcca gcagcgaact ccatgcacct gtgcggatat acgcctgttc cgccagacgt
     1021 aatacttccg gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1081 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1141 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgcgcttc atcaccacgt
     1201 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1261 tttttcgcca tcagcttttc aacttgctga taatcgcctt ccgccagttt cagcagcgcc
     1321 tgttcggtct gcttacgtgc acggcgacgc ttacgcccga caaaccaccc acgggtgtgc
     1381 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1441 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1501 atcagcacat aaccctgatg accggcaatc atcgggccaa ccacgatccc cgcaatcagc
     1561 aacacaaaga gcaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     1621 tatcagcttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag
     1681 gtgcaggtgc aggtgcaggt ttagcttccg ttgtccccgc tgccggttgt gccagcaggt
     1741 tacgcacgcg agtctgcatc agtttttcca gcatcgcctg gctttgcagg gtttccggaa
     1801 gatccatcga gatattttgc tggcttaact ggtctacctc gtcgaggaac gctttggtgg
     1861 tggcatcatc agtatcgtag taagcacgta cccaggtgga gacgttctcc agcgcctggc
     1921 gataagtctc ttgctggtga cgcggtacag cttgtgctgc gaccagcagg cgagagcgaa
     1981 tattttcgcg cagatagata tcctgatttg gcgctaacag cggtacggcg gtgtcatcac
     2041 gacggcgaat cgtaatgaag ttgtccataa agttctgcca gcttttttgc agattgatac
     2101 gccattcgct gatggaactg gaaagctctt caccgtcgga atccatcggc gaaccatcgc
     2161 tgtcattatc ggccagacgc aggttatcta cctgatttga aagctgatta agcttaagga
     2221 tgatgccgtc ataatccacc tgcgatactg cagaaaggct ggcgatatca tcggtaattg
     2281 cccgacgaac ggtaatcaga ctcgggtcat tcatatccgc caggctggcg tctgcacttt
     2341 tcagcaacgc tgcagcggtc gtgacgtcct gatcgctcca cagcttccgt ccggcgagtt
     2401 tcaccagaaa atcggcctga gccagcagcc aggttttagc atcgctgccg gaaatggtgg
     2461 cgaccttttg ttggacttca tccaactgtt ttgccagcgt ttcttgctga cgattcgcct
     2521 gcttaagctg tgcagcttgt tgcttaataa tgccttccag ctcgactttt tggctctcct
     2581 gggctttttg caatgccgtc agttggttag ccagggcatc gctggtggcg gtctgattga
     2641 cggcctgttg tttaccccag ccatacaaac cgacgcccgc cgccagagca atagcgatag
     2701 ccaccgcgct gagaatcaat gcggtattgt tcttactctt tttttctgtt gcgacaggtt
     2761 gtgacgtggt gtccacggcc tccctggtct cttcaaccac ggcggaggtt ttttcttgtt
     2821 ccgtcattat ggcttcctgt tatgagagtt attgtaatgc ccgtaaaagc gcatcgttgt
     2881 cagcgttatc ggcgacctta atgtcttgcc agcccagttc ccgggcgagt ttcgccaaac
     2941 gctcactgac gaccaatagt cgacagtgta gtaaccagtg ctcacgatac cattgtggga
     3001 tcagcgacca gagttgctgc aacatttcac cgctggtaac aacgaccgtc gtcacctcgc
     3061 gggattgcca gcgcatcgct tcttctgcac catcgtaatg gattgcgcat cgttgataac
     3121 attcacaaaa agtgacctca gcaccgcgcg ccgtcagggt atccccaatt agctcacgac
     3181 cgccattgcc acgtaatatc agcgcacgtt tgcccgcaat attttgtaat tcaggtaatt
     3241 gtagcaagac ttcgctgatt tcccgatcct gcgggtagag aatcttctgt ccgcttacgg
     3301 tatgtagtgc cagtgcggtg gtgcgtccaa tggcgaaata attaggtagt cggggccatt
     3361 tacgatcttg ctgatgtagc tgtgattggg caaaagcaac cgcgtgttgc gagagggcaa
     3421 acaacagatc gctctccccc agcgctgcca gttgatcagc aagttgcggt aattgtcgac
     3481 ccggagaaaa ctcaatcagc ggaaaatgcc aggccacctg ccccagtgtg cgcagacggc
     3541 tcactaactc ttctccagcg ggagacgggc gggtgacaag gatactcatg ccggggcgtc
     3601 tccgttatag acttcagcga ggatctcgcg cgcgccgtta ttcagtagct cttctgccag
     3661 cgaaatcccc atttgttcgg catcttgcgg cgcaccgcgg cgttcaccgc gaataatctg
     3721 cgaaccgtcc ggcgcgccga ccagcgcacg cagccagatt tcgccatcaa taagctcggc
     3781 gtagctacca attggcacct gacatccacc ttcgagacgg gtattcatgg cgcgttctgc
     3841 ggtaacgcgc agtgcagttt cgtggtgatt cagcgcggca agcagctcgc gagtgcgtgt
     3901 atcatcaagg cggcattcaa taccgactgc accttgtcct accgccggaa gagaaatctc
     3961 gggtggcaac gcggcgcgaa tgcgggactc cagacctaaa cgttttagtc cggctacggc
     4021 aagaatgatg gcatcgtatt cgccgttatc cagtttgctc aggcgagtgc cgacgttgcc
     4081 gcgcagggag cggataatca gatccgggcg gcgttcagcc agttggcact ggcgacgtaa
     4141 actggacgtc ccgacgatac tgcctgccgg taacgcatcc agactgtcat agttattgga
     4201 cacaaaggca tcgcgaggat cttcacgctc acaaatagtg accaggccca gaccttgcgg
     4261 gaattcaacc ggcacatctt tcattgaatg tacggcgata tcggcgcgat tttcgaggag
     4321 cgcgacttcc agctctttta caaataagcc ttttccgccg actttcgcca gcggcgtatc
     4381 aagaatcaca tcgccgcgcg tcaccatcgg taccagttca acgaccaggc ccggatggct
     4441 cgccatcaac ttgtctttga catagtgtgc ctgccagagt gcaagtgggc tttggcgtgt
     4501 ggcaattctt aaaacattgt ctaacatgct tgttaccgtc attatcatcc gtggtccatc
     4561 ctaacatcct tgccagagtg atgtcagtgt tgtggtgaaa cgtagacgcc tgcgcaaacc
     4621 gtaaaatgag gtctggcagt ggatcctgac aggcgtttca cgccgtcgta ataaggaatt
     4681 tacagagaat aaacggtgct acacttgtat gtagcgcatc tttctttacg gtcaatcagc
     4741 aaggtgttaa attgatcacg ttttagacca ttttttcgtc gtgaaactaa aaaaaccagg
     4801 cgcgaaaagt ggtaacggtt acctttgaca tacgaaatat cccgaatgcc gcgtgttacc
     4861 gttgatgttg gcggaatcac agtcatgacg ggtagcaaat caggcgatac gtcttgtacc
     4921 tctatattga gactctgaaa cagagactgg atgccataaa tcaattgcgt gtggatcgcg
     4981 cgcttgctgc tatggggcct gcattccaac aggtctacag tctactgccg acattgttgc
     5041 actatcacca tccgctaatg ccgggttacc ttgatggtaa cgttcccaaa ggcatttgcc
     5101 tttacacgcc tgatgaaact caacgccact acctgaacga gcttgaactg tatcgtggaa
     5161 tgtcagtaca ggacccgccg aaaggtgagc ttccaattac tggtgtatac accatgggca
     5221 gcacctcgtc cgtagggcaa agttgttcct ctgacctgga tatctgggtc tgtcatcaat
     5281 cctggctcga tagcgaagag cgccagttgc tacaacgtaa atgcagcctg ctggaaagct
     5341 gggccgcctc gctgggtgtg gaagttagct tcttcctgat tgatgaaaac cgttttcgcc
     5401 acaacgagag tggcagtctg gggggcgaag attgtggctc cacccagcat atactgctgc
     5461 ttgatgaatt ttatcgtacc gccgtgcgtc tcgccggtaa gcgtattctg tggaatatgg
     5521 tgccgtgcga cgaagaagag cattacgatg actacgtaat gacgctctac gcacaaggcg
     5581 tgctgacgcc aaatgaatgg ctggatctcg gcggcttaag ctcgctttcc gctgaagaat
     5641 acttcggtgc cagcctttgg caactctaca agagtatcga ttctccatac aaagcggtgc
     5701 tgaagacact gctgctggaa gcctattcct gggaataccc gaacccacgc ctgctggcga
     5761 aagatatcaa acagcgtttg cacgacggcg agattgtatc gtttggcctc gatccttact
     5821 gcatgatgct ggagcgtgtt actgaatacc tgacggcgat tgaagatttc acccgtctgg
     5881 atttagtacg tcgctgcttc tatttaaaag tgtgcgaaaa gctcagccgt gaacgcgcct
     5941 gcgtaggctg gcgtcgcgca gtgttgagcc agttagtgag cgagtggggg tgggacgaag
     6001 ctcgtctggc aatgctcgat aaccgcgcta actggaagat tgatcaggtg cgtgaggcgc
     6061 acaacgagtt gctcgacgcg atgatgcaga gctaccgtaa tctgatccgc tttgcgcgtc
     6121 gcaataacct tagcgtctcc gccagtccgc aggatatcgg cgtgctgacg cgtaagctgt
     6181 atgccgcgtt tgaagcatta ccaggtaaag tgacgctggt aaacccgcag atttcacccg
     6241 atctctcgga accgaatctg acctttattt atgtgccgcc gggccgggct aaccgttcag
     6301 gttggtatct gtataaccgc gcgccaaata ttgagtcgat catcagccat cagccgctgg
     6361 aatataaccg ttacctgaat aaactggtgg cgtgggcatg gtttaacggc ctgctgacct
     6421 cgcgcacccg cttgtatatt aaaggtaacg gcattgtcga tttgcctaag ttgcaggaga
     6481 tggtcgccga cgtgtcgcac catttcccgt tgcgcttacc tgcaccgaca ccgaaggcgc
     6541 tctacagccc gtgtgagatc cgccatctgg cgattatcgt taacctggaa tatgacccga
     6601 cagctgcgtt ccgcaatcag gtggtgcatt ttgatttccg taagctggat gttttcagct
     6661 ttggcgagaa tcaaaattgc ctggtaggta gcgttgacct gctgtatcgc aactcgtgga
     6721 atgaagtgcg tacgctgcac ttcaacggcg agcaatcgat gatcgaagcc ctgaaaacta
     6781 ttctcggcaa aatgcatcag gacgccgcac cgccagatag cgtggaagtc ttctgttata
     6841 gccagcatct gcgcggctta attcgtactc gcgtgcagca actggtttct gagtgtattg
     6901 aattgcgtct ttccagcacc cgccaggaaa ccgggcgttt caaggcgctg cgcgtttctg
     6961 gtcaaacctg gggcttgttc ttcgaacgcc tgaatgtatc ggtacagaaa ctggagaacg
     7021 ccatcgagtt ttatggcgct atttcgcata acaaactgca cggcctgtca gtgcaggttg
     7081 aaaccaatca cgtcaaatta ccggcggtgg tggacggctt tgccagcgaa gggatcatcc
     7141 agttcttttt cgaagaaacg caagatgaga atggctttaa tatctacatt ctcgacgaaa
     7201 gcaatcgggt tgaggtgtat caccactgcg aaggcagcaa agaggagctg gtacgtgacg
     7261 tcagtcgctt ctactcgtca tcgcatgacc gttttaccta cggctcaagc ttcatcaact
     7321 tcaacctgcc gcagttctat cagattgtga aggttgatgg tcgtgaacag gtgattccgt
     7381 tccgcacaaa atctatcggt aacatgccgc ctgccaatca ggatcacgat acgccgctat
     7441 tacagcagta tttttcgtga tgaacgtgcc ggaaagcgag gcttatccgg catgcaatct
     7501 tagcggaaac tgactgtttc acccgcctgc tgcgtcgccg cctgttccag caaatcccgg
     7561 aaggtttcgc cgctgcgatc gcaaatccac tcatcgcctt tcagatcaaa atggtagccg
     7621 ccttgtttgg ttgccagcca tacctggtgc agcggttcct ggcggttgat aatgattttg
     7681 ctgccattct caaaggtaat ggtcagcacg ccgccgttga tttcgcagtc gatatcgctg
     7741 tcgccatccc agtcgtccag acgctcttca atggtcagcc acagttgatc agccaggcga
     7801 tgaaattcac tgtcgttcat tgttgtatcc tgtttttaag tgatggcggc agtatagcgg
     7861 catggggtca gggcttcaaa gtttgcacct ctgcggctgc gttacggcat gattcatccg
     7921 tcaccggaat aatgatgtct ctgtgtagcg aaagatttgt ctcttcatta gggcgcagtt
     7981 acaccacgcc tttccctgtt tctggtaaac attatgatca ggtttaccga gcgagcatcc
     8041 tcacgctgac ggaactaaaa aagccaacaa acaaaccaca ttgcgatagt gcataaagcc
     8101 atcctggcgt gaggtgttga tcacgaaact gccagcagaa cataaatcct cacgagtaag
     8161 cgttatactc gcagcatttc ctcacttttc agacttcata aagagtcgct aaacgcttgc
     8221 ttttacgcct tctcctgcga tgatagaaag cagaaagcga tgaactttac aggcaatcca
     8281 taatgaaaaa cgtgtttaag gcactcactg tattacttac tctcttcagc ctgacgggct
     8341 gcggtctgaa aggtccgctc tatttcccgc ctgcagataa aaatgcaccg ccgccgacca
     8401 aaccggtaga gacgcaaacg caatccacgg tgccggataa aaacgatcgc gccactggcg
     8461 atggtccatc ccaggtgaat tactaaaagt cagtttctgt acccgcgtga ttggagtaaa
     8521 tgatgcagtt ctcgaaaatg catggccttg gcaacgattt tatggtcgtc gacgcggtaa
     8581 cgcagaatgt ctttttttca ccggagctga ttcgtcgcct agctgatcgg cacctggggg
     8641 tagggtttga ccaactgctg gtggttgagc cgccgtatga tcctgaactg gattttcact
     8701 atcgcatttt caatgctgat ggcagtgaag tggcgcagtg cggcaacggc gcacgctgct
     8761 ttgcccgttt tgtgcgtctg aaagggctga ccaataagcg cgatatccgc gtcagcaccg
     8821 ccaacgggcg gatggttctt accgtcaccg atgatgatct ggtccgcgta aatatgggcg
     8881 aacccaactt cgaaccttct gccgtgccgt ttcgcgctaa caaagcggaa aagacctata
     8941 ttatgcgcgc cgccgagcag acaatcttat gcggcgtggt gtcgatggga aatccacatt
     9001 gcgtgattca ggtcgatgat gtcgatactg cggcggtaga aacactgggg cctgttctgg
     9061 aaagccacga gcgttttccg gagcgcgcca atatcggttt tatgcaagtg gttaagcgcg
     9121 agcatattcg tttacgcgtt tatgagcgtg gggcaggaga aacccaggcc tgcggcagcg
     9181 gcgcatgtgc ggcggttgca gtagggattc agcaaggttt gctggccgaa gaagtacgcg
     9241 tggaactccc aggcggtcgt cttgatatcg cctggaaagg tccgggtcac ccgttatata
     9301 tgactggccc ggcggtacat gtctacgacg gatttattca tctatgaagc aaccagggga
     9361 agaactgcag gaaacactca cggagcttga tgaccgggtg gttgtcgatt atctgattaa
     9421 aaatcctgag ttttttatcc gcaatgcgcg cgcagtagaa gcgatacgtg tgccgcatcc
     9481 ggtacgcggt accgtttcgt tggtcgagtg gcacatggcc cgcgcacgta atcatattca
     9541 tgttctggaa gagaacatgg cgctgttgat ggaacaggct ctcgccaacg aaggcctgtt
     9601 ttatcgctta ctctacctgc agcgcagtct caccgccgcc agcagtcttg atgatatgct
     9661 gatgcgcttt caccgctggg cgcgtgatct cggcctggca ggtgcgagtc tgcgcctgtt
     9721 tccggatcgc tggcgcttag gtgcgccgtc gaaccacact catctggcat taagccgtca
     9781 gtctttcgaa ccgctgcgta ttcagcgtct ggggcaggag cagcattatc tggggccgct
     9841 taacggaccg gagctgctgg tggtactacc ggaagcgaaa gcggtgggat cggtggcgat
     9901 gtcgatgctg ggaagcgatg ctgatttagg cgtggtgctg ttcaccagcc gcgatgccag
     9961 tcactatcaa caagggcagg gaacgcagtt acttcatgaa attgcgctga tgttgccgga
    10021 gcttctggag cgttggattg aacgcgtatg accgatttac acaccgatgt agaacgctac
    10081 ctacgttatc tgagcgtgga gcggcagctt agtccgataa ctctgcttaa ctaccagcga
    10141 cagcttgagg cgatcatcaa ttttgccagc gaaaacggcc tgcaaaactg gcagcaatgc
    10201 gatgcagcga tggtgcgcaa ttttgctgta cgcagtcgcc gtaaagggct gggcgcggca
    10261 agcctcgcat tacgcctctc tgcgctacgt agcttttttg actggctggt cagccagaac
    10321 gaactcaaag ctaacccggc gaaaggtgtt tcggcaccga aaacgccacg tcatctgccg
    10381 aaaaatatcg acgtcgatga tataaatcgc ctgttggata ttgatatcaa cgatcccctc
    10441 gctgtgcgcg accgtgcaat gctggaagtg atgtacggcg cgggcctgcg tctttccgag
    10501 ctggtggggc tggacatcaa acacctcgac ctggagtccg gcgaagtgtg ggtgatgggg
    10561 aaaggcagca aagagcgccg cctgccgatt ggtcgtaacg ctttgtcatg gattgagcac
    10621 tggcttgatt tgcgcgacct gtttggtagc gaagacgacg cgctttttct gtcgaaactg
    10681 ggcaagcgta tctccgcgcg taatgtgcag aaacgctttg ccgaatgggg cataaaacaa
    10741 gggctgaata atcacgttca cccgcataaa ttacgtcact cgttcgctac gcatatgctg
    10801 gagtcgagcg gcgatcttcg tggtgtgcag gagctgctgg gtcatgccaa cctctccacc
    10861 acgcaaatct atactcatct tgattttcaa caccttgcct cggtgtacga tgcggcgcat
    10921 ccacgcgcca aacgggggaa ataatgcgtt tttaccggcc tttggggcgc atctcggcgc
    10981 tcacctttga cctggatgat accctttacg ataaccgtcc ggtgattttg cgcaccgagc
    11041 aggaggcgct tacctttgtg caaaattacc atccggcgct gcgcagcttc cagaatgaag
    11101 atctgcaacg cctgcgccag gcggtacggg aagcggaacc cgagatttat cacgacgtga
    11161 cgcgctggcg ttttcgctca atagaacaag cgatgcttga cgccggattg agcgcggaag
    11221 aagccagtgc aggcgcacac gcggcaatga tcaactttgc taaatggcgc agcaggatcg
    11281 acgtcccgca gcaaacccac gacaccttaa aacagctggc gaagaaatgg ccgctggtgg
    11341 cgatcaccaa cggtaacgct cagccggaac tgtttggttt gggggattat tttgagtttg
    11401 tgctgcgcgc tggcccgcac gggcgctcga aaccgttcag cgatatgtac ttcctggcgg
    11461 cggaaaaact caacgtgccg atcggcgaga tcttacatgt aggtgacgat ctcaccactg
    11521 acgtgggtgg ggcaattcgc agcggaatgc aggcctgttg gatcagaccg gaaaatggcg
    11581 atctgatgca aacctgggat agccgtttac tcccgcatct ggaaatttcc cggttggcat
    11641 ctctgacctc gctgatataa tcagcaaatc tgtatatata cccagctttt tggcggaggg
    11701 cgttgcgctt ctccgcccaa cctattttta cgcggcggtg ccaatggacg tttcttacct
    11761 gctcgacagc cttaatgaca aacagcgcga agcggtggcc gcgccgcgca gcaaccttct
    11821 ggtgctggcg ggcgcgggca gtggtaagac gcgcgtactg gtgcatcgta tcgcctggct
    11881 gatgagcgtg gaaaactgct cgccatactc gattatggct gtgacgttta ccaacaaagc
    11941 ggcggcggag atgcgtcatc gtatcgggca actgatgggc accagccagg gcggcatgtg
    12001 ggtcggcacc ttccacgggc tggcgcaccg tttgctgcgt gcgcaccata tggacgccaa
    12061 tctgccgcag gatttccaga tcctcgatag cgaagaccag ctacgcctgc ttaagcgtct
    12121 gatcaaagcc atgaacctcg acgagaagca gtggccgccg cgccaggcaa tgtggtacat
    12181 caacagccag aaagatgaag gcctgcgtcc gcatcatatt caaagctacg gtaatccggt
    12241 ggagcagacc tggcagaagg tgtatcaggc gtatcaggaa gcgtgcgatc gtgcaggcct
    12301 ggtggacttc gccgagctgc tgctgcgcgc tcacgagttg tggcttaaca agccgcatat
    12361 cctgcaacac taccgcgaac gttttaccaa catcctggtg gacgaattcc aggataccaa
    12421 caacattcag tacgcgtgga tccgcctgct ggcgggcgac accggcaaag tgatgatcgt
    12481 cggtgatgac gaccagtcaa tctacggctg gcgcggggcg caggtggaga atattcagcg
    12541 tttcctcaat gatttccccg gtgccgaaac tatccgtctg gagcagaact atcgttctac
    12601 cagtaacatt ctgagcgccg cgaacgccct gattgaaaac aacaacgggc gtctgggtaa
    12661 aaaactgtgg accgatggcg cggacggtga gcctatttcc ctctattgcg cttttaacga
    12721 actcgatgaa gcgcgttttg tcgttaaccg catcaaaacc tggcaggaca acggtggggc
    12781 gctcgccgag tgcgccattc tctaccgcag caacgcccag tcgcgggtgc tcgaagaggc
    12841 gttattgcag gccagtatgc cgtaccgtat ttacggcggg atgcgcttct tcgaacgcca
    12901 ggaaatcaaa gatgcgctct cgtatctgcg cctgattgcc aaccgcaacg acgacgcggc
    12961 ctttgagcgt gtggtgaata cgccaacgcg gggtattggt gaccggacgc tggacgtggt
    13021 acgtcagaca tcgcgcgatc gccagttaac actctggcag gcatgtcgtg aactgttgca
    13081 ggaaaaagcc ctcgccggac gtgctgccag cgccttacag cggtttatgg aactgatcga
    13141 cgccttagcg caggaaactg ccgatatgcc gctgcatgta cagactgacc gggtaattaa
    13201 agactccggc ctgcgtacca tgtacgagca ggagaagggc gaaaaaggtc agacgcgtat
    13261 cgaaaactta gaggaactag tgacggcaac gcgccaattc agctacaacg aagaagacga
    13321 agatttaatg ccgctgcagg cgttcctctc ccatgcggcg ctggaagcgg gcgaggggca
    13381 ggcggatacc tggcaggatg cagtgcagtt gatgacgctg cactcggcga aaggcctgga
    13441 gttcccgcag gtgtttatcg tcggtatgga agagggcatg ttcccaagcc agatgtcgct
    13501 ggatgaaggc ggacgtctgg aagaagaacg ccgtctggcc tacgttggtg taacccgcgc
    13561 gatgcagaaa ctgacgctga cctacgcgga aactcgccgt ctgtatggca aagaggttta
    13621 ccatcgcccg tcgcgcttta tcggtgagtt gccggaagag tgtgtggaag aggtgcgcct
    13681 gcgcgccacg gtaagccgcc cggtcagcca tcagcgtatg ggtacgccga tggtcgagaa
    13741 cgacagcggc tacaagctcg gccagcgtgt acgccacgct aagtttggtg aaggcactat
    13801 cgtcaatatg gaaggcagcg gcgagcatag ccgtttgcag gtggcattcc agggccaggg
    13861 tattaaatgg ctggtggcgg catacgcccg gctggagacg gtataacgtt gcgggatgcg
    13921 gtgcgccaca ccgcatccgg catcagcacc ttacttatca catttcttca catcaatatt
    13981 ccagtcagca ccagacgcaa cctgatgttt ctgcgcgaaa ttatccatat tcagcgccac
    14041 ggaaacattc aataagctgt tcagatacac catcggctgt ccttccggca catcgccgaa
    14101 gcttgcgaca tacggcattt tccctgcgta tttttgccgg gaaccttcgg aaatggtcac
    14161 acacaacgtg tcgttgagtt tgatccccgc ctgattcagc aactcgtcgc tgatattgct
    14221 ccagacgttg ccgtactgaa tatcgagaat cgggatattg cccttcactt caccttttgt
    14281 tgcttttgct ttctgataag aaagttcgac aacctttgca ggaagctcag gccccacctg
    14341 ctcgaacgtg atcgccccgg aagccagacg tgcaccggtg taggcataca cgtcacgacc
    14401 gtggaaggta tatgactttt cagaaccttt caggcggtta gctttttcgt cgatttcccg
    14461 gacgctctca atacccagcg attccgccac caaagtgagc gtaccgttat ccggtgaaac
    14521 gaagtattga ccgtttttgg ttttcagcac tatcgattta cggtcagtgc ctacgcccgg
    14581 atcgaccaca ctgacaaata ccgaaccttg cggccagtaa ctggcggtct gatacaggcg
    14641 gtaagcgcct tcccagatgt tatacggtgg aatttcgtgt gttaaatcaa atattttaag
    14701 attatggtca acaccgaatg cgaccccttt catcgccgag acggcaccat ctttcaggct
    14761 aaaatcagtt tgtaaaatca aagcgttatc cgcataagcg gatgatgatc ccagacacgt
    14821 taataaaaat aacgttgcga gcgttttttt catttttgtt tcccttcatt tatctgatga
    14881 ttttccagct gtgtagccag ccccagtttg tctggcctcc tcgagtgtac aatgcttaac
    14941 ctttccgctc gacggaaatc atggtgacaa aaggatagcg ttgccacggg attgccccgc
    15001 ctttcatata catatgtgaa atcgtgccat caagataaag cagctgctca acgttcagtt
    15061 tcgctttggc ataacaggca aaatcataaa aatttgtcgc ctgctggctc aacaaaaaca
    15121 cggcattccc atgtttatta attccaacac cgttacgaat tttacgtgag gcgacgttgg
    15181 gatgaatacg cggattaatt acactgtttt ccatcaacat tggccctgac tgcaccgcaa
    15241 actgaatctc tttactggtt ttgaaggcat ccagacgaac gatgccgact ttatctcccg
    15301 cgacataaaa cacgccgcca ggacggataa agaaattccc ttcacctgaa gcgagattta
    15361 acgccacctt ctgctgaccg ttttcgatgt acaaaccgag cggcgcatag ctttcatcat
    15421 agatgccgcc gttcatcgcc atctgcacct gaccctgact attcatatcc gccagcagag
    15481 catgtaaagt tccccacgct tcgccattgg ctttttgcca gtacattttc acccgctctg
    15541 tttgaggatt aacggtatac gcctgtacgg tcagcgttgg atctgagagt gcgcaatcat
    15601 cagcggcaac agcaaacaag ggaagtaagg tgagggcgag aaaaatccgt ttgagattca
    15661 aggtgatcat tcctttacca atgagtagcc gatgcgccat tataggtcct ggatgtggga
    15721 tttttttatc ctgttagcga ccttgacgag taccaaaaag cgcgaagttc aactattgtt
    15781 ctgtggtgtt ctgttgcgtg ttgacggcaa aattttgctg gcgtaacatg cgcgcacgat
    15841 cactctaaga ggacattcgc cttggacaca cccagtagat actggctcac tatcctgtca
    15901 tccaggatca actcctaagg ctatcccttt ttgctgatag ccttagcggt tgtcagcgac
    15961 ctcaattttt cccgtcgcgc tgagtcaggc tgtttaatgg tctgaaaccc aatttgtttc
    16021 tgtgtgccca ccgaactgtc cgatatttta agcattggga gtcccggtca tgctgagcgc
    16081 atttcaactg gaaaataacc gactgacccg gctggaagtc gaagagtcac aaccccttgt
    16141 aaatgcagta tggattgatc ttgtcgaacc ggacgacgac gagcgactgc gcgtacaatc
    16201 tgaacttggc cagagcctgg caacccgccc ggaactggaa gacatcgaag catcggcacg
    16261 tttctttgaa gacgatgacg gcctgcatat tcactccttc ttcttctttg aagatgcgga
    16321 agatcacgcc ggtaactcca ctgtggcatt taccatccgt gatggtcgtc tgtttactct
    16381 gcgtgagcgt gaactgcccg cttttcgtct gtatcgtatg cgtgcccgta gccagtcgat
    16441 ggtagacggt aacgcctacg agttgctgct ggatctgttc gaaaccaaaa tcgaacagtt
    16501 ggcagatgaa attgaaaata tctatagcga cctggagcaa ttgagccggg tgattatgga
    16561 agggcatcag ggcgatgagt acgacgaggc gctctccact ctggcggaac tggaagatat
    16621 cggctggaaa gtgcgcctgt gtctgatgga tacccagcgc gcgctcaact tcctggtacg
    16681 taaagcacgc ttaccgggtg ggcaactgga gcaggcgcgt gaaatcctgc gagatatcga
    16741 atccctgctg ccgcataacg aatccctgtt ccagaaggtg aacttcctga tgcaggcggc
    16801 aatgggtttt atcaacatcg agcagaaccg catcatcaaa attttctcgg tggtatccgt
    16861 cgtgttcctg ccgccaacgc tggttgcttc cagctacggg atgaactttg agtttatgcc
    16921 agaactgaag tggagtttcg gctacccagg cgcgattatc tttatgatcc tcgcgggcct
    16981 ggcaccgtat ctgtacttta agcggaagaa ctggttgtaa tatgagtgcc ggataaagct
    17041 atttttttat ccggcttatt tctaaaaatt gtctttttta aagttatgcg gattgaaaac
    17101 ttgctctatt ttattttctt gtgttaattg atactcagta aaatcgttaa taccaatatg
    17161 agtgttacga taagggtatt cattaataac cattaagtaa tttttcgtga tgcaattatc
    17221 attgctctta attgtcggga aacagtagag atcttcatac ggcccttcga tatatttttt
    17281 atagtcagtg aagccattac cgatatcaat gacataccag gtatgccaac taccttctgg
    17341 taggggatgt tgatttttaa tttcattctg atgcttcaga taccaattta ttttatcgct
    17401 gtcagtccat ggcagatgat caacgaccag aaagactgaa ttgccagcat cagagtatgt
    17461 atacaaaatc tgtacaggac gcaatgtcca taaattatag atgcagcata gaagaaataa
    17521 acataatata attttttgga gtttgctttt catagataat tttcgattct ttcgatagtg
    17581 ttaaaattgg tgaaaaaagg tttgaaagca aagtctttat gccgttgtaa aaagaaccat
    17641 atgcgaaaaa atctatattt gctgtaagtc ttgtttttga tatcagttac atcaagaccg
    17701 aagtggtcct gtgctacaaa atgaattgtt gcactccatc ctatagcgta tttttgaaaa
    17761 cttaaaagag atattttttg agctgaaata tcatgaacac tgattcctaa gccattaaaa
    17821 ctgtcctcga agtcattaaa tttatttagt cttgatgaaa gtaatttggt tttaatgctt
    17881 tgcaggataa gaggttggta tcctgtcgag ggagttgaat taatgccatt ttcgatacat
    17941 tctttgatta
//
LOCUS       DABSYT020000090        17729 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.090, whole genome shotgun sequence.
ACCESSION   DABSYT020000090 DABSYT020000000
VERSION     DABSYT020000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.090"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..304)
                     /locus_tag="ID725_RS18265"
                     /old_locus_tag="pgaptmp_003653"
     CDS             complement(<1..304)
                     /locus_tag="ID725_RS18265"
                     /old_locus_tag="pgaptmp_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGV7852160.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYR"
     gene            complement(288..611)
                     /locus_tag="ID725_RS18270"
                     /old_locus_tag="pgaptmp_003654"
     CDS             complement(288..611)
                     /locus_tag="ID725_RS18270"
                     /old_locus_tag="pgaptmp_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7852161.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            736..1212
                     /gene="racR"
                     /locus_tag="ID725_RS18275"
                     /old_locus_tag="pgaptmp_003655"
                     /gene_synonym="rarC"
     CDS             736..1212
                     /gene="racR"
                     /locus_tag="ID725_RS18275"
                     /old_locus_tag="pgaptmp_003655"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HGV7852162.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1528..1680
                     /locus_tag="ID725_RS18280"
                     /old_locus_tag="pgaptmp_003656"
     CDS             1528..1680
                     /locus_tag="ID725_RS18280"
                     /old_locus_tag="pgaptmp_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGV7852163.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(1795..2310)
                     /locus_tag="ID725_RS18285"
                     /old_locus_tag="pgaptmp_003657"
     CDS             complement(1795..2310)
                     /locus_tag="ID725_RS18285"
                     /old_locus_tag="pgaptmp_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852164.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            2434..2832
                     /locus_tag="ID725_RS18290"
                     /old_locus_tag="pgaptmp_003658"
                     /pseudo
     CDS             2434..2832
                     /locus_tag="ID725_RS18290"
                     /old_locus_tag="pgaptmp_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            2894..3163
                     /locus_tag="ID725_RS18295"
                     /old_locus_tag="pgaptmp_003659"
     CDS             2894..3163
                     /locus_tag="ID725_RS18295"
                     /old_locus_tag="pgaptmp_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGV7852165.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            3132..4250
                     /locus_tag="ID725_RS18300"
                     /old_locus_tag="pgaptmp_003660"
     CDS             3132..4250
                     /locus_tag="ID725_RS18300"
                     /old_locus_tag="pgaptmp_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGV7852166.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            4417..5211
                     /gene="potC"
                     /locus_tag="ID725_RS18305"
                     /old_locus_tag="pgaptmp_003661"
     CDS             4417..5211
                     /gene="potC"
                     /locus_tag="ID725_RS18305"
                     /old_locus_tag="pgaptmp_003661"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGV7852167.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            5208..6254
                     /gene="potD"
                     /locus_tag="ID725_RS18310"
                     /old_locus_tag="pgaptmp_003662"
     CDS             5208..6254
                     /gene="potD"
                     /locus_tag="ID725_RS18310"
                     /old_locus_tag="pgaptmp_003662"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGV7852168.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(6410..7231)
                     /gene="cobB"
                     /locus_tag="ID725_RS18315"
                     /old_locus_tag="pgaptmp_003663"
     CDS             complement(6410..7231)
                     /gene="cobB"
                     /locus_tag="ID725_RS18315"
                     /old_locus_tag="pgaptmp_003663"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGV7852169.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(7247..8158)
                     /gene="nagK"
                     /locus_tag="ID725_RS18320"
                     /old_locus_tag="pgaptmp_003664"
     CDS             complement(7247..8158)
                     /gene="nagK"
                     /locus_tag="ID725_RS18320"
                     /old_locus_tag="pgaptmp_003664"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGV7852170.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(8187..9431)
                     /gene="lolE"
                     /locus_tag="ID725_RS18325"
                     /old_locus_tag="pgaptmp_003665"
     CDS             complement(8187..9431)
                     /gene="lolE"
                     /locus_tag="ID725_RS18325"
                     /old_locus_tag="pgaptmp_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGV7852171.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(9431..10132)
                     /gene="lolD"
                     /locus_tag="ID725_RS18330"
                     /old_locus_tag="pgaptmp_003666"
     CDS             complement(9431..10132)
                     /gene="lolD"
                     /locus_tag="ID725_RS18330"
                     /old_locus_tag="pgaptmp_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGV7852172.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(10125..11324)
                     /gene="lolC"
                     /locus_tag="ID725_RS18335"
                     /old_locus_tag="pgaptmp_003667"
     CDS             complement(10125..11324)
                     /gene="lolC"
                     /locus_tag="ID725_RS18335"
                     /old_locus_tag="pgaptmp_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGV7852173.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            11586..12659
                     /gene="ycfT"
                     /locus_tag="ID725_RS18340"
                     /old_locus_tag="pgaptmp_003668"
     CDS             11586..12659
                     /gene="ycfT"
                     /locus_tag="ID725_RS18340"
                     /old_locus_tag="pgaptmp_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGV7852174.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            12787..16233
                     /gene="mfd"
                     /locus_tag="ID725_RS18345"
                     /old_locus_tag="pgaptmp_003669"
     CDS             12787..16233
                     /gene="mfd"
                     /locus_tag="ID725_RS18345"
                     /old_locus_tag="pgaptmp_003669"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGV7852175.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            16377..17339
                     /gene="ldtC"
                     /locus_tag="ID725_RS18350"
                     /old_locus_tag="pgaptmp_003670"
     CDS             16377..17339
                     /gene="ldtC"
                     /locus_tag="ID725_RS18350"
                     /old_locus_tag="pgaptmp_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGV7852176.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(17422..17679)
                     /gene="bhsA"
                     /locus_tag="ID725_RS18355"
                     /old_locus_tag="pgaptmp_003671"
                     /gene_synonym="ycfR"
     CDS             complement(17422..17679)
                     /gene="bhsA"
                     /locus_tag="ID725_RS18355"
                     /old_locus_tag="pgaptmp_003671"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGV7852177.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4520 a         4547 c         4427 g         4235 t
ORIGIN      
        1 cacggtaata ttcagagctg tgactgcgca tacgggccac cagcagaggc ggcattgcct
       61 tttcgatcgc cggtaacaga gcctgcattt tttcaacagc atcaggggtg tctttatcca
      121 gccaacggaa aattttctgg gtattacgag ccagggcttc cggatggctg tcgtcgtaca
      181 gttccgggaa cgtcatcccc agttcgaaat aagtccgggc tatttcagct gcaggaactt
      241 tctcaccatc aggatatgcc caggcattca tcgccatgcg gatgtgctca tgtttgattt
      301 tcatgaatca tttgcctctt gatgcttcgg gtatgatcgt tttcgtcatt tggttgcttc
      361 atcgacatat tctgcgaata acatgacgag cgtcgtaagt atgtccaatc aacatcagga
      421 cgaagttctt cacacaggac accacctttt gttgctcgtt caatcgcagg acatctctca
      481 gcaggcaact gacgtacacc tttgatccat tgatttacgc ttggaggaga tacacctaaa
      541 agcctagcca ttgctgattg cccaccgaca acagcacaag ctcgtttgaa tgaatagtta
      601 tcttttttca tcgaatgaac tccaaaaaac acacaataat attaggctta gcctaatgca
      661 actgtcaata ggctatgcct aacgcttcga gagtaggtat tgcctaatgc gatgcgcata
      721 ggagactatt aagcaatgct tagtggtaaa gacttaggcc gagcgataga gcaggccatt
      781 aacaaaaaaa ttgcatcagg agccgtcaaa tcaaaggcgg aaatcgcacg tcatttcaaa
      841 gtccaaccac catcaatcca tgactggatt aagaaaggtt cgataagtaa agacaaactt
      901 ccagaactat ggcgtttctt ttctgatgtg gttggtccag agcattgggg gcttaacgaa
      961 taccccatac caaccccatc cacttcagat acaaaaagtg aacttttaga cataaacagc
     1021 ctttatcaag ccgcctctga tgaaaaaaga gcaattgtgg ctttcctctt atctggaaat
     1081 gctacggagc ctagttgggt tgatcatgac gttcgtgcct acattgccgc aatggaaatg
     1141 aaggtagcta actatctgaa aaatcaagaa tcaaaacgga aaagccagaa catcaccaag
     1201 acaggaactt aaacttatat ggtccgacgg gaaattccta gttcccgtta gttaactcct
     1261 actacctctc ccacaaacca tcacctatta ggtcgcaccc aaatcattag gcgtagccta
     1321 ttgacaagca attaggcatt tcctatagtt ttcccatacc aacccatccc gtcccacaca
     1381 atacagggca atacctagag ttacccggca gtggtcaggg attaagtagc cagcccgagg
     1441 cgtaagaaca tgacggcagg gttcaacttt aactatgcag caggtttttg ttccgctacc
     1501 ccggcgttaa ggggatatga ggtcagcatg gatactcttg atcttggcaa caacgaatct
     1561 ctggtgtgcg gcgtgtttcc caaccaggac ggcacgttca ccgcgatgac gtataccaaa
     1621 agcaaaacgt ttaaaaccga atctggcgcg cgtcgctggt tagcaagaaa ctctgactaa
     1681 tgaaatctgg taattaagga atcctccacg gggggagagt ggtgcacacg cgccggacac
     1741 aagcaagcat ccggcatgct ctttaacaat ctggatatcc ataacagtaa aaatctacag
     1801 attgccgttc agttttctgg ccaactcttc aatagatgga ggtgatacgt aatccggatt
     1861 tttattcata agaaactgat tttcacagta caggcacctg cttttatgaa aaaattcata
     1921 ttctctaacc gggaatggct gaagtatcga tactatcttt tgtccaaaac attttggaca
     1981 aagatgcatg attatgctgc cgccgttcac ggttacctcc ttcgagtaca caaaagttcc
     2041 cgactcaagt tggctaagga tataaccttc cgtctgagcc tcaaagtttt caaattctgc
     2101 aattttagct ttgagagaag catttatttc ttgataagtg cccaccagct caacgagaga
     2161 cacgcattcg cgctgaatag atgcaagctt tgagttcagt tcaccaatag ccgcatttac
     2221 ttcagcttga gtttttgcct cgttcattag ttttgcaatc tgggcggttt cacgaatagc
     2281 cgtcattgct gccgttaatt cagcgatcac attcaatact cttattgttg ttggggatat
     2341 ccagattaac aagatccttg ttgttgggga ataacgggga ccacctcgcc tgacgtggta
     2401 aaaagcaggc acacaacacg aaagcacacg gcgagggaca ccattaaatg gtggcttaag
     2461 caatcacgcg aagcgcaatc tgccattatg accgatgaaa tcccgttaga tgatgcactg
     2521 ttacaattgc gggaatttat cgacgaaaac tccggtgaat tttttgttca ggtctgggga
     2581 aatggagcca acttcgacaa cacgattttg cgccgttcat acgaacggca ggggatcccc
     2641 tgcccgtggc gttactacaa cgatcgcgat gtacgcacaa tcgttgagct ggggaaagcc
     2701 atagacttcg atgccagaac ggctattcca ttcgaaggtg agcgccataa tgcacttgat
     2761 gacgcccgtt accaggcaaa atacgtttca gttatctggc aaaaactgat cccgagtcag
     2821 gctgattttt aatgttcaac cgtcgccagt tgtcgttgat attctgcaac tggcgcgttc
     2881 cggagtgata gccatgagcg aacagtacct gataacgctc gacgagtgga aaccaaaacg
     2941 gttcagtctc ccaataacaa acactaccct ggtgaaatac ggaaaactag gatacatcgt
     3001 tccaagacca caaaaaattc gtgggcgttg gctgatagat cgccgagcag tatttgttgg
     3061 gcctggtgaa acgggaattg cgccggaaat tcatactggc gatgatgatg cactgaagga
     3121 gattttaact catgtcaccg aggccacgaa aaaacagcac tgacgtagcc ggtctttacg
     3181 aaaagtttga tcgcagaact ggcagagttt actaccagta taaaaatcct gtgactggaa
     3241 aatttcacgg actcggaaca gacaaaggta aggcagaaaa aatcgcttcc acagccaatc
     3301 agcgaatagc tgcagcagaa gctgaatatt tcatgcgcaa aattgatgaa agtccgtcag
     3361 caacaaaacg tcggggtatc agattaaagg catgggttga tcgatatctg aaaatacagg
     3421 acacgcgact gaaaaatgga gatattgcag ctacaactca caaagaaaaa actcgaatgg
     3481 ctgcatacct ggtttcccgt ctgggaaacc acccattgaa agaactggaa gtaagagact
     3541 ttgcattaat actggatgag tggctggata aagacatggt cagtacagcg agagtaaatc
     3601 gtggattatg ggttgatatt tataaagaag cacagcatgc aggggaagtt cctcctggat
     3661 ggaatcctcc ggaggctacc cgtaaaccga tccctaaagt aaccagagcc aggctcacca
     3721 tggaagactg gcaaaaaatt tacaatgcaa cgcctgaaaa acactttatc cgtaacgcaa
     3781 tgcttcttgc gattgttact ggtcagcgcc gtgatgacat ttgccacatg cgtttttcag
     3841 atgtgtggaa cgaacacttg catatcaccc agggaaaaac cggaatgcgt ctggcgttac
     3901 cgcttacact acgctgtgat gccattggga taacgttaaa agaagttatt gatgggtgcc
     3961 gagacagaat attaagtcca tatctaatcc atagtcggca ccagaaacaa ccgaagccga
     4021 tgagtaaaga caacctgagc gactactttg ccaaagcacg ggatctggct ggggtaattc
     4081 caccagcagg aaaaactccg ccaacatttc atgaacaacg ctctctatca gaacggctgt
     4141 accgtgcaca gggtatcgat acaaaaacat tactaggaca taaagtccag gcaaccaccg
     4201 atcgctataa cgatactcga ggtcaggaat gggttaagtt ggttatttga caaacaccat
     4261 atggaatatc cgtcaccatc tcaggaaaaa agtgttgtaa aatgcgtggc ctagttttgg
     4321 ggaaacgttt tggagagatt ttggagaaag gaaaaaattg aataaattca aaatcctgaa
     4381 agtgtcccgc cttatgaata aaaaggtgga actcgaatga tcggtcgact gcttcgcggc
     4441 ggttttatga ccgctatcta cgcgtacctg tatatcccaa tcattatttt gattgtgaac
     4501 tcctttaaca gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa atggtatagc
     4561 ctgctgatga acaacgacag cctgttacag gcagcgcagc attcactaac aatggcggtg
     4621 ttttcggcga cgtttgctac gcttatcggt tcactgacgg cagttgcgct gtaccgttat
     4681 cgctttcgtg gtaagccgtt cgttagcgga atgctgtttg tggtgatgat gtcaccagat
     4741 atcgtgatgg cgatttcgct gctggtgctg tttatgctgc tgggtattca gcttggcttc
     4801 tggtcgctgc tgttctcgca tatcaccttc tgcctgccat ttgtggtggt gacggtgtat
     4861 tcgcgcctga aaggttttga cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc
     4921 gaatttacca ttctgcgaaa aatcattctg ccactggcaa tgccagcggt ggcggcaggc
     4981 tgggtgttaa gctttaccct gtcgatggac gatgtggtgg tttcttcgtt cgtcaccggg
     5041 ccgagttatg aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg
     5101 gaagttaacg cgctggcaac catattactg gtgctgtcgc tggtgatggt tattgccagc
     5161 cagcttattg ctcgtgataa aacgaaaggt aacacagggg acgttaaatg aaaaaatggt
     5221 cacgccacct gctcgcggcg ggtgctctgg cactgggcat gagcgccgct cacgccgatg
     5281 acaacaacac gctgtatttc tacaactgga ccgagtacgt gccgccagga ctgcttgaac
     5341 agttcaccaa agaaaccggt attaaggtta tctattcgac ttacgagtcg aacgaaacca
     5401 tgtatgccaa gctgaaaacg tacaaagacg gtgcctatga cctggtggtt ccttcaacct
     5461 attacgtcga taaaatgcgt aaagaaggga tgatccagaa gatcgacaag tcgaagttaa
     5521 cgaacttcag caatctcgat ccagacatgc tcaataagcc gtttgacccg aataacgatt
     5581 actccattcc gtatatctgg ggtgcgacgg cgattggcgt taacggtgat gcggtggatc
     5641 cgaaatctgt caccagctgg gccgatttgt ggaagcctga gtacaaaggc agcctgctgc
     5701 tgaccgacga tgcccgtgaa gtgttccaga tggcgctacg taagctgggc tactccggta
     5761 acaccaccga tccgaaagag attgaagctg catataacga gctgaaaaaa ctgatgccaa
     5821 acgtcgcagc gtttaactcc gataacccgg ctaacccgta catggaaggc gaagttaacc
     5881 tcggcatgat ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg cctattgacg
     5941 tggtgtggcc gaaagaaggc ggcatattct ggatggacag cctggcgatc ccggcaaatg
     6001 ccaaaaacaa agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa
     6061 aacaggttgc tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa
     6121 gtccagaagt ggcgaacgat aagacgctct ccccggatgc tgaaaccatt aaaaatggcg
     6181 aatggcagaa tgacgttggc gcagccagca gcatttatga agagtattat cagaagctga
     6241 aagcaggacg ttaattcagc aagcgggcgg tggtattacc gcccgttttt tagttagcaa
     6301 ggatgtggct aatgagtcag gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg
     6361 tccgcttgta ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt tataatccct
     6421 tcagcaactt ttcaacaaat tctggcacca cctggcttgc cgggccgtaa tatttctcgg
     6481 caaattcatt accgacctga cttggttcaa gattcagctc cacggtgtgc gcgccatgca
     6541 gtttcgcttc gtgaacaaac ccagccgccg gataaacatg cccggaagta ccaatggcaa
     6601 tgaaaatatc ggccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc
     6661 caaaccatac tacgtgtggg cgcaaggggg ccgggaactg gcagcaatgg catttatctt
     6721 ctggggtaac gtctccggtc cagtcgagaa cctgaccact ttgtgaacaa cgaactttca
     6781 gcagttcccc atgcatatga atcacattgg tattacctgc gcgttcatgc aggttgtcta
     6841 tattctgcgt caccagcaaa aagcgatcgc cgagagcatc ttgcagttta gccagcgcaa
     6901 gatgcgcggc gttaggctga atttctggct gctgcagctg tcgacgacgg gcgttataaa
     6961 acgcttgcac cagttcagga tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc
     7021 gatgttcttc ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa
     7081 ttcctgcccc tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat
     7141 ctctgaaaaa aatacgctga cgcaaacgct cgcgcaggcg gcgtttattt ttacgaaaac
     7201 gacttaaccg atgaccccga cgcgacagca tagcaacctc tgttgtttaa tcggttagat
     7261 gtaggaaggc cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg
     7321 gaacacgagc tacaggtaag agatgacgag gcagcctgtc cgccagttgc gttgtgattg
     7381 ccgggaaatt cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga
     7441 tatttcccag acaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct
     7501 catcgccttg atcataaagc gcaataattt cgggagccgg caacggttga tgataatagt
     7561 gttgatacag ccacgcaaaa ccgcgaccag acagataatt ttcaatgcag ccatgctgac
     7621 cacagccgca gcggcgtaac gggaaatcca gccccatcat ggttaacgca tcaaccggca
     7681 gacgcatatg gccaaactcg ccggtaatgt agcttttccc ggtaatcggt ttgccgttga
     7741 aaatcagccc gccgccaacg ccggtgccga gaatcaaccc catcaccaac ggatattgcg
     7801 taaattcgtc atcccaggct tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta
     7861 catcgcgatc aagacgtgcg ctcaggtcgg cacgcagcgg tttaccgctg gcagcaggga
     7921 cattggcggc atacagcgta ccatcttctg tttccggcat tcccggaata ccgatgccga
     7981 cagagccttt acagccaaaa cgttgatcag cttcggctac cagctcgcac actgcatcta
     8041 aaaatgcgtc atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac
     8101 cgctatcaaa cacgccaagc gcaatttttg ttccaccaat atcaaacccg taatacattg
     8161 ccgctccttg ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata
     8221 ttactggcgc gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc
     8281 agtacgtaga agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaatagata
     8341 tcgctggaga ggaactgatg accgatcagc ttttcaatcc gctcaataat cggggtaagt
     8401 tgcagtgaaa caactacgcc gataatcaca ccacacaggc taccgaatag ccctgccagc
     8461 aatccatacc agacaaagat ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat
     8521 actgcgatat cgccactctt gtctttcacc gccatcacta aggtggagac gatgttgaaa
     8581 caggccacgc caatcaccag taccatcgcc agatacataa tggcgcggat catctggata
     8641 tcgcgataca tatagccgta agtaccaatc cagcttttaa tataaacata gctgttggtc
     8701 acttcacccg catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca
     8761 atacctgaca cgctggaacc catatcaaga tattgttggg catccgccag cgggatcatg
     8821 gcaaaactgt gatcgagttg accactcaac tgcaaaatac cggcaacgtg caaacgcaca
     8881 cgttttggct gcatcagttt atgctcagga ttcgagttgg ggatcataat cgacacccaa
     8941 tcgccctgct tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg
     9001 cccgctttaa aattgcgcca ggcatccccc tgaacaaacg agggtaatgc gctcagacgc
     9061 tgttcctgtt gcgggttaac gcccttcacc tggattgcgc gcagattcgc tccactttcc
     9121 accagcccgg tgaaattgat atacggcgca gcggcggcaa tacctggcac tttttgcacg
     9181 ttatccagtg cttcctgcca gttagtccac ggttgatcca ccgcctctat ttcgccatgc
     9241 ggcaccaccg ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag
     9301 ccgacgatca ataccgccac gccaagggca atgccaatgg tagaaatgac ggagatcagc
     9361 gacaccatgc cgccgcgtcg ccgtccgcgg ctaaaacgca ggccaatcaa taacgataaa
     9421 ggcatcgcca ttactccgcc cccatcaggc tcagttccgc cgtcagacga ccatcacgca
     9481 tctccagttg gcggctcata cgtttcgcca gttgcaggtc gtgagtaacc accaggaagg
     9541 cggtgccctg caagcgattc aattccccaa gcaactggaa gatgctgtct gcgttacgcg
     9601 catcgaggtt accggtaggt tcatccgcca gtaccagcca cgggttattg accagcgcac
     9661 gggcaatcgc cacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac
     9721 gatgctccag ccccaccgct tttaacatct caagtgcacg gctgttgatt tcagcgggct
     9781 ttttcttgcc aatcagcagc ggcatagcca cgttttccag ggcagtaaaa tccggcagca
     9841 ggtggtgaaa ctgataaata aagcccagct tctggttgcg cagttctgct ttcgccgccg
     9901 aagacagttt gctcatcggt tgaccgttaa agatcacatc gccggaggtt ggtgtatcca
     9961 gcccgcccag caggtgcagc aaggtacttt taccggaacc agagctaccg acgatcgcca
    10021 tcatttcacc ttcgcccacg ctgaaactga cattgtgcag tacatcggtt tgcacactgc
    10081 cttcctgata gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa
    10141 gcctcagcgg gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg
    10201 atagccatcg ccaccagcgc aataacaatg acctgtaaag gttcgatagc caccggcagc
    10261 gccgcgccat caagcaggac gccgattatc ggcatcagat tatttaactg gctggcaagc
    10321 agtgcgccaa gcgccgctcc gaggatcgca ccgataatcc cggcgctggc cccttgcacc
    10381 ataaagacca tcatgatttg tcgcggagtt aagccttgcg tttgcaggat cgctacttcg
    10441 ccctgcttct ccatcaccat cagccccagc gaggtaataa tgttaaacgc cgcaacggcg
    10501 acaatcaggc tcagcagtaa gcccatcata tttttttcca tgcgtacggc ctggaacagc
    10561 tcgcctttac gatcacgcca gtcctgccat ttgctgcctt caggcagttt ttgctgactt
    10621 aaagagtcaa ctttcagcgg ctcatccagc cacaaacgcc agccggtaat attgcctgcc
    10681 ggataacgca tcagacgcga ggcatcctca atattcacca gcatttcata gccatcgact
    10741 tcactgttag cggcaaaagt accaatcaca ttgaacaggc gctgacttgg aatacgcccc
    10801 atcggcgtga actggctggc agatggcacc atcacgcgga tttgatcacc gcgattaacg
    10861 cctagctgtg aggcaagttg ttcgccgagg atgacattat atttccccgg ctcgaggtca
    10921 gtttgtttca cattgaccag atacggcgtt agtggatctt tttgcgccgg atcgataccc
    10981 agcatcaccc cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata
    11041 ggtgcgacgc gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta
    11101 agagagccat gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc
    11161 agctcgcgct caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg
    11221 gtaatgccga tggtagaaag ccaggagacg aaacgaccga agcgatccgc tgcacgccca
    11281 cgcatgtaac gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg
    11341 ctgtagcaaa gacacgagta tataagcgaa agcaagatgg gtaaacgtct gaactgtaag
    11401 tgatcgtcag ggctaatctg aaaagccccc gtaaatcaat cggctaaatt gccatgtttt
    11461 atcatcaatc ttgccgcctg atcttaatct gaaaaatcat ccaatacaga cagataccca
    11521 ttacacctcg ggtcataacc cgcaggatca accgcatgta aattaaaaaa gagcgtgttg
    11581 tcctgatgaa acaaaaagag ctatggatta accagatcaa agggttatgt atttgtctgg
    11641 tggtgattta tcactcggtc attacctttt atccgcatat gaccactttt cagcatccgt
    11701 tatcggaagt cctgagcaaa tgctggatct atttcaatct ttaccttgca ccctttcgta
    11761 tgccggtttt tttctttata tccggctatt tgattcgccg ctatatcgac agcgtaccgt
    11821 ggggaaattg tctcgataaa cggatctgga acatcttctg ggtgctggcg ctttggggcg
    11881 tggtgcagtg gctggcgtta agtgcgctga accagtggct ggcacctgag cgcgatttaa
    11941 gcaatgcctc caatgccgcg tatgccgatt ccaccggtga gttcctgcac gggatgatca
    12001 ccgccagcac cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa
    12061 tttttagccg tctggcgctg ccactattcg ccttgtttgt actgctgagt gtggcggtta
    12121 atttcgttcc cacgccgtgg tggggaatga acagtgtgat ccgcaatttg ccttattaca
    12181 gccttggtgc atggtttggc gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc
    12241 gccatctgct gatggcttct ttgctggctg ctctggcggt cggtgcctgg ttgtttacta
    12301 tctcgctgct gttgtcgctg gtgtcgattg tggtgatcat gaagttgttt tatcagtatg
    12361 aacaacgttt tggcatgcgt tccaccagtc tgctgaatgt gattggctcc aacactattg
    12421 ctatctacac tacccataga attctggtag agatattcag cttaactctg cttgcgcaaa
    12481 tgaacgcagc acgctggtcg ccgcaagtcg aactgacact cctgctggtt tacccctttg
    12541 ttagtttgtt catctgtact gttgcgggct tgctggtaag aaaactttca cagcgagcat
    12601 tcagcgatct gttgttctcc ccgccttcac tgcccgttgc cgtcagttac tcccgctaag
    12661 ccatcgcccc agccggggcg attcaatttg ctaaaccatg tcgattacgg ataatgaaca
    12721 gcattgctta tcagagatat gcccccatat gttgaggcat atcctaacga gaatctgaca
    12781 accgttatgc ctgaacaata tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg
    12841 ctgggcgagt taaccggcgc tgcctgcgcg acgctggtag cggaaattgc cgaacgtcac
    12901 gccggaccgg tggtgcttat tgcgccagat atgcaaaatg ccctgcgttt gcatgatgaa
    12961 attagccagt ttaccgacca gatggtgatg aatctggcgg actgggaaac cctcccttac
    13021 gacagttttt cgcctcatca ggacattatc tcctcgcgcc tttccaccct ttaccagcta
    13081 ccgacaatgc agcgtggcgt actgattgtt ccggtgaata cacttatgca gcgcgtttgc
    13141 ccgcacagtt ttctccacgg tcatgcgctg gtgatggaaa aaggtcagcg cctgtcacga
    13201 gatgcattac gaacccaact ggatagcgcc ggttatcgcc atgttgacca ggtgatggag
    13261 cacggcgaat acgccacgcg cggcgcgttg ctggatctct tcccgatggg gagtgagttg
    13321 ccttatcgtc ttgatttctt tgatgatgaa atcgacagtc tgcgggtgtt tgacgtcgac
    13381 agtcagcgca cgctggagga agtagaagcg atcaacctgc tgcctgcgca cgaatttccg
    13441 accgataaag cggcgattga actgttccgc agccagtggc gcgatacctt cgaagtgaag
    13501 cgcgatccgg aacatattta ccagcaagtg agtaaaggca cattacctgc cgggatcgag
    13561 tactggcagc cattgttctt cagcgaacca ctgccgccgc tgttcagtta tttccctgcc
    13621 aataccttgc tggtgaatac tggcgatctg gaaaacagtg ccgaacgttt ccaggctgac
    13681 acgctggcgc gttttgagaa tcgcggcgtc gatccgatgc gcccactgtt gccaccacaa
    13741 tcgctctggc tgcgggtgga cgagctcttc tcagagctga aaaactggcc ccgagtgcag
    13801 ctaaaaactg aacatttacc gacaaaagcc gcgaatgcca atttaggttt ccagaaactg
    13861 ccagacctgg ccgttcaggc acaacaaaaa gcgccgctgg atgcgctgcg taagttcctc
    13921 gagtctttcg acggtccggt ggtgttctcg gtagaaagtg aaggccgccg tgaagcgctg
    13981 ggtgaactgc tcgcgcgaat taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc
    14041 agcgaccgtg ggcgttatct gatgattggc gctgcagagc atggttttgt cgatacggta
    14101 cgtaatctgg cgctgatttg cgaaagcgat ctgctcggtg aacgcgttgc ccgtcgtcgt
    14161 caggattctc gccgcaccat caaccccgat acactgatcc gtaacctcgc ggaactgcat
    14221 attggtcagc cggtggtcca tctggagcac ggcgtcgggc gctatgccgg aatgaccacg
    14281 ctggaagcag gcggcattac cggcgagtat ctgatgctca cctatgccaa cgacgccaaa
    14341 ttgtatgttc cggtgtcgtc gctgcatctg attagccgtt acgcgggtgg tgcggaagaa
    14401 aatgccccgc tgcataaact tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg
    14461 gaaaaagtgc gtgatgtggc ggcggaattg ctggatatct acgcgcaacg cgccgccaaa
    14521 gagggcttcg cgtttaaaca cgatcgtgag cagtatcagt tgttctgcga cagcttcccg
    14581 tttgaaacca cgccggatca ggcgcaggcc attaatgcgg tacttagcga catgtgtcag
    14641 ccgctggcaa tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg
    14701 atgcgcgccg ctttcctggc agtagataac cacaagcagg tggcggtgct ggtgcctacc
    14761 acccttctcg cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa ctggccggta
    14821 cgtatcgaaa tgctctcccg tttccgcagc gccaaagagc agacgcaaat ccttgcggaa
    14881 gtggcggaag ggaaaatcga tattctgatc ggtacgcaca aactgctgca aagtgacgtc
    14941 aagtttaaag atttaggcct gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat
    15001 aaagagcgca ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg
    15061 atcccacgta cgctgaatat ggcaatgagc ggaatgcgtg acctatcgat tatcgccacg
    15121 ccgcccgccc gtcgtctggc agttaaaacc tttgtccgtg agtatgacag cctggtggtc
    15181 cgcgaggcga tcctgcgtga aattttgcgc ggaggacagg tttattatct ctacaatgat
    15241 gtggaaaaca ttcagaaagc cgccgaacgg ctggcagaac tggtgccaga agcgcggatc
    15301 gccatcggtc acgggcagat gcgcgagcgc gaactggaac gggtgatgaa tgatttccat
    15361 catcaacgtt tcaacgtgct ggtttgtaca accattatcg aaaccgggat cgacatcccg
    15421 acagccaaca ccattatcat tgaacgcgcg gatcacttcg gtctggcgca actgcaccag
    15481 ttacgcggtc gcgtcggacg ttcgcatcat caggcatatg catggctgct gacgccgcat
    15541 ccaaaagcga tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat
    15601 ctcggggcag gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg
    15661 cttggcgaag aacaaagcgg ctcaatggaa accatcggtt tctcgctgta tatggagttg
    15721 ctggaaaacg ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga agatctcacc
    15781 agccagcaaa cagaagtcga gctgcggatg ccgtcgctat tgccagatga tttcattcct
    15841 gacgtgaata cgcgtctgtc gttctataaa cgtattgcca gcgccaaaac ggaaaacgaa
    15901 ctggaagaga tcaaagtcga gcttatcgat cgcttcggcc tgctgccgga tccggcgcgt
    15961 accctgctgg atattgcccg tctacgccag caagcgcaga aactggggat caggaagctg
    16021 gaaggtaatg agaaaggcgg cgtgatcgaa tttgccgaga agaatcacgt taatccggcc
    16081 tggttgattg gtttgctgca aaaacagccg cagcattacc gccttgatgg tccgacgcgc
    16141 ctgaaattta ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt
    16201 atgcgtgaac tggaagaaaa cgcgatcgct taatggtaaa tcgggggcgt ttctgcgccc
    16261 ccatctgcaa catttacaaa ttctttgcac ttccctgcac tatccgacac tgtcaccatc
    16321 cataattcag gcttatctgt ttattacaat aaccttatat ttattatgga tttttggtga
    16381 tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct gccgtggcgc
    16441 tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc agcgggcagt cgtctggttg
    16501 gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc atcgccaaaa
    16561 aatataacgt cggctttctc gctctattac aggctaaccc cggcgttgat ccttacgtac
    16621 cgcgcgcggg cagcgtgtta acgatcccgt tgcaaaccct acttccagac gcgccgcgcg
    16681 aaggcattgt gatcaacatt gcggagcttc gtctctatta ctacccgccg ggtaaaaatt
    16741 cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg acaccgacaa
    16801 tggtgaccac cgtttctgac aagcgtgcaa acccaacctg gacgccaacg gcaaacattc
    16861 gcgcgcggta taaggcacaa ggaattgagt tgcctgcggt agtgccggct ggaccggata
    16921 atccaatggg ccatcatgcg attcgtctgg cggcctatgg cggcgtttat ttgcttcacg
    16981 gtacgaacgc cgatttcggc attggcatgc gggtaagttc tggctgtatt cgtctgcggg
    17041 atgacgatat caaaacactc tttagccagg tcaccccagg caccaaagtg aatatcatca
    17101 acactccgat aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc
    17161 cgctgtcaga gaagattgat gacgatccgc agctgctgcc aattacgctg aatagcgcaa
    17221 tgcaatcatt taaagatgca gcacaaactg acgctgaagt gatgcaacat gtgatggatg
    17281 tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa acgctgtaaa
    17341 tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca
    17401 ggcattaatg agggttaatg cttatttata aattactgct gttccatgga gggtattcgg
    17461 accggttaca gaagtaatac ggaaagattt tgcgcccatc tcatccgctt tttgcgccag
    17521 ctgctcttcc agcgatccca gatttgtccc cgcgttagca ctgattgtac cgactttttg
    17581 ttggccttct ggcgttgact gaacttcgac agccgcaaag ctggcaaatg acatggagct
    17641 taaaatcgcc gcagcgatga gggtttttac gtttttcata atagtggcct tatgcagatg
    17701 aatgacgtcg caagcaatta cttaacgat
//
LOCUS       DABSYT020000091        17399 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.091, whole genome shotgun sequence.
ACCESSION   DABSYT020000091 DABSYT020000000
VERSION     DABSYT020000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.091"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(53..784)
                     /gene="rlmB"
                     /locus_tag="ID725_RS18360"
                     /old_locus_tag="pgaptmp_003672"
     CDS             complement(53..784)
                     /gene="rlmB"
                     /locus_tag="ID725_RS18360"
                     /old_locus_tag="pgaptmp_003672"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGV7852178.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(965..3406)
                     /gene="rnr"
                     /locus_tag="ID725_RS18365"
                     /old_locus_tag="pgaptmp_003673"
     CDS             complement(965..3406)
                     /gene="rnr"
                     /locus_tag="ID725_RS18365"
                     /old_locus_tag="pgaptmp_003673"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGV7852179.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3445..3870)
                     /gene="nsrR"
                     /locus_tag="ID725_RS18370"
                     /old_locus_tag="pgaptmp_003674"
     CDS             complement(3445..3870)
                     /gene="nsrR"
                     /locus_tag="ID725_RS18370"
                     /old_locus_tag="pgaptmp_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGV7852180.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGLMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4075..5373)
                     /gene="purA"
                     /locus_tag="ID725_RS18375"
                     /old_locus_tag="pgaptmp_003675"
     CDS             complement(4075..5373)
                     /gene="purA"
                     /locus_tag="ID725_RS18375"
                     /old_locus_tag="pgaptmp_003675"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGV7852181.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5477..5674)
                     /gene="yjeT"
                     /locus_tag="ID725_RS18380"
                     /old_locus_tag="pgaptmp_003676"
     CDS             complement(5477..5674)
                     /gene="yjeT"
                     /locus_tag="ID725_RS18380"
                     /old_locus_tag="pgaptmp_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGV7852182.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5756..6760)
                     /gene="hflC"
                     /locus_tag="ID725_RS18385"
                     /old_locus_tag="pgaptmp_003677"
     CDS             complement(5756..6760)
                     /gene="hflC"
                     /locus_tag="ID725_RS18385"
                     /old_locus_tag="pgaptmp_003677"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGV7852183.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6763..8022)
                     /gene="hflK"
                     /locus_tag="ID725_RS18390"
                     /old_locus_tag="pgaptmp_003678"
     CDS             complement(6763..8022)
                     /gene="hflK"
                     /locus_tag="ID725_RS18390"
                     /old_locus_tag="pgaptmp_003678"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGV7852184.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8108..9388)
                     /gene="hflX"
                     /locus_tag="ID725_RS18395"
                     /old_locus_tag="pgaptmp_003679"
     CDS             complement(8108..9388)
                     /gene="hflX"
                     /locus_tag="ID725_RS18395"
                     /old_locus_tag="pgaptmp_003679"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGV7852185.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9465..9773)
                     /gene="hfq"
                     /locus_tag="ID725_RS18400"
                     /old_locus_tag="pgaptmp_003680"
     CDS             complement(9465..9773)
                     /gene="hfq"
                     /locus_tag="ID725_RS18400"
                     /old_locus_tag="pgaptmp_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGV7852186.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9859..10809)
                     /gene="miaA"
                     /locus_tag="ID725_RS18405"
                     /old_locus_tag="pgaptmp_003681"
     CDS             complement(9859..10809)
                     /gene="miaA"
                     /locus_tag="ID725_RS18405"
                     /old_locus_tag="pgaptmp_003681"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGV7852187.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10802..12649)
                     /gene="mutL"
                     /locus_tag="ID725_RS18410"
                     /old_locus_tag="pgaptmp_003682"
     CDS             complement(10802..12649)
                     /gene="mutL"
                     /locus_tag="ID725_RS18410"
                     /old_locus_tag="pgaptmp_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGV7852188.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12659..13996)
                     /gene="amiB"
                     /locus_tag="ID725_RS18415"
                     /old_locus_tag="pgaptmp_003683"
     CDS             complement(12659..13996)
                     /gene="amiB"
                     /locus_tag="ID725_RS18415"
                     /old_locus_tag="pgaptmp_003683"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGV7852189.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14015..14476)
                     /gene="tsaE"
                     /locus_tag="ID725_RS18420"
                     /old_locus_tag="pgaptmp_003684"
     CDS             complement(14015..14476)
                     /gene="tsaE"
                     /locus_tag="ID725_RS18420"
                     /old_locus_tag="pgaptmp_003684"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGV7852190.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14448..15995)
                     /gene="nnr"
                     /locus_tag="ID725_RS18425"
                     /old_locus_tag="pgaptmp_003685"
     CDS             complement(14448..15995)
                     /gene="nnr"
                     /locus_tag="ID725_RS18425"
                     /old_locus_tag="pgaptmp_003685"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGV7852191.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15994..17133
                     /gene="queG"
                     /locus_tag="ID725_RS18430"
                     /old_locus_tag="pgaptmp_003686"
     CDS             15994..17133
                     /gene="queG"
                     /locus_tag="ID725_RS18430"
                     /old_locus_tag="pgaptmp_003686"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGV7852192.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKRLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17116..17169)
                     /locus_tag="ID725_RS18435"
                     /old_locus_tag="pgaptmp_003687"
     CDS             complement(17116..17169)
                     /locus_tag="ID725_RS18435"
                     /old_locus_tag="pgaptmp_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852193.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3944 a         4968 c         4372 g         4115 t
ORIGIN      
        1 tagacaaaaa aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc
       61 gctgacgaac cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa
      121 cacttcctgc cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca
      181 taccttcacc ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctttgataga
      241 gcgtatgatc cgcctcacct gccgtaccga cgatccagat attctcttcc tgcaacatac
      301 gcatggtgcg cgcaaggtta gtcacccgaa tcagtggaac gctttctgcc gcgccgcagg
      361 ctactttttt cgccgtggcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcgt
      421 gaacgccagc agcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga tcggttacac
      481 cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt
      541 cctggtactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc
      601 ttttctcgtc gagatattgg cggtttgcca actggataac cacgccctgg gactcaaggg
      661 cgtggatcag cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa
      721 aacgttcagg ggcgcgctcc agcagggcct gcactgcatg gatgccgtaa atcatttcgc
      781 tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggtctacatg
      841 atccctgcaa tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca
      901 tccggcatga acaaagcgca ttttgtcagc aatctaaccc tcttctgttt gaagagggta
      961 atgatcactc tgccactttt ttcttcgccg cacgcttcgc tttggtcgct gcagctattt
     1021 tctgcgtttt cgccgatggc tttttcgctt ttctcgcgtc tttcttcgct gctttcggct
     1081 tcgttttttt ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt ttaccgacct
     1141 gacgacgctt gccgcctttt ttacctgcat cgcctttttt cgctttctcg cgcgccgttt
     1201 taccgacgtt gcgcggtgcg cgttcgctgg agatcaggct aaagtcgatt ttgcgctcgt
     1261 ccatattaac cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa gtctggccgc
     1321 tggattcacc catcaggcgt tgccctacct ggtcaaagcg atagtagtca ttgtccagcg
     1381 aagagacatg gaccagccca tcaatgaaca agtcgtccag acggacgaag aagccaaagc
     1441 cagtgacgct ggaaattacg cctttaaaga cgttacctac ctggtcgagc atgaagtcac
     1501 acttcagcca gtcagacaca tcgcgcgttg cttcgtcggc acgacgttcc gccatcgaac
     1561 agtgctgacc caattgcagc atctcttcca tcgaataatg gtagccaccg gtttcagtgg
     1621 tgttgccctg atgcccctgc tctttcgcca gcagatactt aatggcgcgg tgcagcatca
     1681 ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat gccaggccaa
     1741 agtgaccacg gttttccgga tcgtaaatcg cctgtttcat cgagcgcagc agcatggttt
     1801 gcagcatttc tgcatcagga cggtcggcaa ccgactccag cagctccgcg tagtcgcgcg
     1861 gttccggctt gttaccgccc ggcagttcca gccccagctc tgccagcact gaacggaaag
     1921 aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt tctttcgctt
     1981 tctcaacgaa acgcgccgcc gagatattcg ccagaatcat gcactcttca attaacttgt
     2041 gtgcgtcgtt acgctgagtc tgttcgatac gttcaatacg gcgttcagcg ttgaaaatga
     2101 acttcgcttc ttcgctctca aatgagatcc cgccgcgttc ttcacgggct ttatccagca
     2161 ctttatagag gttatgcaac tcttcgagat gcttaaccag cggggcgtac tgctcgcgca
     2221 gatcctgatc gccctgcaga atatgccaga ctttggtgta ggtcagacgc gcgtgagagc
     2281 tcatcaccgc ttcgtagaat ttgtagccag tcaggcggcc tttcgacgaa accgtcatct
     2341 cgcacaccat acacaggcgg tctacctgcg gattgagcga gcacagaccg ttagagagca
     2401 cttccggcag catcgggata acctgcgaag ggaagtacac cgacgtacca cggttacgcg
     2461 cttctctgtc cagcggcgtt ggcggacgca catagtagct gacgtcagca atcgcgaccc
     2521 ataaacgcca gccgccgccg cgttttttct cgcagtaaac cgcatcgtca aagtcacggg
     2581 cgtcttcgcc atcaatggtg accagcggta aatcgcgcaa atcgacacgg cccgcttttg
     2641 cttcttccgg cacttcttct ttcagcccag caacctgttg ctcaacagcc tgcggccaga
     2701 tgtacggaat ttcatgggta cgcagagcga tatcaaccgc catgcccgtg cccatattgt
     2761 cgcccagcac ttcgacgatt ttacccaccg ctttggtacg gcgagtcggg cgttgggtca
     2821 gttcaacaac caccacaaag cccatccgcg cgcccatgat ctgatcgggc gggattaaga
     2881 tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct tcggtaaagt
     2941 agcgaccaac aatctggctg gtttttggca ccagtacgcg gacaatacgc gcttcacgac
     3001 gacctttacg gtcagcaccc agcggctgag ccagcacctg atcgccatga atgcaggttt
     3061 tcatctgctc gctggagaga tacaaatcat ctttacgccc ttcaacccgc agaaagccgt
     3121 agccatcacg gtggccaata acggtacctt tcaccaggtc gaggcgttcc ggcagcgcat
     3181 agcactgacg acgagtaaag accagttgac catcgcgctc catcgcgcgc aggcggcgac
     3241 gcaggccttc aagctgctct tcgccttcaa tgtgcagttc taccgccagc tcatcacggc
     3301 tggccggttt ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta gggatgggat
     3361 tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg tgacatctcg gttcctccgt
     3421 tgtcatctct gatgaagatt ttcgtcactc caccagcaat aatttataaa gcggttgatt
     3481 ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac tttgcacggc
     3541 cttagaaagc gcctgtttca accgacaggc aggtgtaatg tggcaaaact cactgctgca
     3601 attcaccagc gataagggct ccagctcgcg caccacatca ccaatacgta tcgcactcgc
     3661 cggtttaccc aggcgaatgc cgccattttt tccacgaaca gcagtcacgt agccggcacg
     3721 actaagttga ttgattattt tgaccatatg attacgggag acgccgtaga cgtcagtcac
     3781 ttcagaaata ctggtcatca gcccttctgg caatgacgcc atgtagatca gcgcacgtaa
     3841 tccgtaatca gtgaaactcg ttaactgcac atcaacctca aaagggaaat cgggaaaaaa
     3901 tacatttata ttgatgataa accagccaca agctgtgtcg ctaatttatt tcagttcggg
     3961 gaaggaaaaa agcgaggatt taacactgtg tcggatagcg taatggctta accgacacag
     4021 caatgtatca cactgttagc ccggcaggca aaatatctgc caggcgtacc agaattacgc
     4081 gtcgaacggg tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag agatgatatc
     4141 gatcggtaca ccggtcagct cttcaatgcg cttgatgtag ttcagcgccg cctgcggcag
     4201 gccgctacga tctttcacgc cgaaggtgga ttcagaccag cccggcatgg tttcgtaaat
     4261 cggctctaca cctttccagt cgtcagctgc cagcggagtg gtagtcactt cacgaccatc
     4321 cggcatacgg taagccacac agagtttcac ctctttcagg ccatccagaa cgtccagttt
     4381 agtcaggcag aagccagaca gggagttcag ctgtaccgca cgacgaacgg caacggtgtc
     4441 cagccagccg gtacgacgac gacgacccgt agttgcgccg aattcgttac cctgcttgca
     4501 gaggaactcg ccagtttcat caaacagttc agtcgggaac ggacctgcac ccacacgagt
     4561 ggagtaagct ttgaggatac ccagaacgta atcaacataa cgcgggccca ggccggaacc
     4621 ggtcgccacg ccaccagcag tggtgttgga agaagttacg tacggataag taccgtggtc
     4681 gatatccagc agcgtaccct gcgcaccttc gaacatgacg aaatcgccac gctgacgcgc
     4741 ctggtcgagc aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg caacagccat
     4801 cgtatcatcc agaactttct ggtaatcaac cgcttcagct ttgtagtagt taaccaactg
     4861 gaagttgtga tattccatca cttctttcag tttttcagcg aaggtttctt tgtcgaaaag
     4921 gtcgccaaca cgcagaccgc gacgtgccac tttatcttca taagcaggcc cgataccacg
     4981 accggtggtg ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat ccagcgcaac
     5041 atgataatca aggatcagcg gacatgcttc agacagcagc agacgctcac gaacggggat
     5101 gccacggtct tccagttctt tcatctcttt catcagcgcg gccggagaca gcacaacacc
     5161 gttaccgatg atgctagtta cattctcgcg gagaatacct gatggaataa gatggagaac
     5221 ggttttttca ccgttgatta cgagagtatg gcctgcgttg tgaccgccct ggtagcgtac
     5281 aacatattta gcccgttcag tcagaagatc gacgatctta cctttacctt cgtcacccca
     5341 ttgggtgccc agtacgacga cgttgttacc catttttcaa aatcaccgtt tgcttaaaaa
     5401 tggattctac catcgctttt tcagagttac agcacttttt gcactcaaaa atgacctcga
     5461 tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat gtagtagacc acaacgcccg
     5521 caaccacaag tccaccgcca aaacgacgta aaatattatc gggcaaattg gtcatcgcag
     5581 agatcatctt cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc agtaccaaaa
     5641 ccagggcaag cgccagccag attgtcgaat tcattcatta tccttataga aaaagaaaac
     5701 caccgacatc cctgaggatg cggtggcttt attgacctgt accgcagtcg ttatattaac
     5761 gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg ctcatgacca
     5821 tcacgtcctg attgccagag aagctgttct cataagcacg caggctacgg atgaatgcgt
     5881 agaagtccgg atctttactg aatgcatcag caaacagttt ggctgcttcg gcatcgcctt
     5941 caccgcgcat gatgcggccc tgacgctcag cttctgccag cgttctggtc acttcatagt
     6001 cggcagtcgc gcgcagtttt tccgcttctt cctgaccttg tgaacggtga cgacgcgcta
     6061 ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc agacacttcg gtcggcaggt
     6121 tgatctgctt aattcgcaca tcgacaactt caatacccag cgccgccata ctgttcgggt
     6181 tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg ctctgccgct tcggcaatgg
     6241 cgttatctgc cgccggggta gtaacttcat cttctgtacc cgcagaaccg gagttcagcg
     6301 cgtcacgtac ttcgagggtc agacgaccac gggaatcggt gacgatgtct ttcacgtcca
     6361 ggcgaccaat ttcagaacgc agacggtcag agaacttacg tttcaacagc acttccgctt
     6421 gcgaaatgtc gccccccccc gttgccaggt agtaacggct gaaatcgctg atgcgccatt
     6481 tgatgtaaga gtcgacgatc aggtctttct tctctttggt cacaaagcgg tcggcctggt
     6541 tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac ggtatcttga
     6601 aatgcagacc cggctcataa accagaggtt tgttgtcatc gtcacgcagt accttaccaa
     6661 aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa gacagacatg taaagcacta
     6721 ccagcacgat gatgataatc gcgataactg acttacgcat cgttattccc cctgacgctg
     6781 gtagtcgtta cgctgcgcat tggcgcggcg ttggtccata atatcgccct gactggtgga
     6841 cgacgtgttg cttgctccac tggttgtgga ggaagaggct ggcggcagac gcagcagatt
     6901 gctggcaccg ttatcgctct tcgccgcagg ggcgttacca cctttcagca tctggtctaa
     6961 cggcagaacc atcaggttgc cacctttatc gttaaccagc actttgcggg tgttacccaa
     7021 cactttttcc atcgtctcga tatacagacg ctcgcgagta atttccggcg cggctttata
     7081 ttccggcaga agtttagcaa aacgcgccac ttcaccctga gcttccagga tggtctgggc
     7141 cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac gcggctgaac
     7201 ttcgttggta tacgcttctg cttcacgaat gtattgctgt tcgttttcac gcgcggcaat
     7261 cgcatcgtca aacgccgctt ttacttcttc cggcggacga gcagcctgga agttgacgtc
     7321 cagcagcgtg atacccatgt catacggacg aatcgtctct tccagttcgc gctgagtatc
     7381 gctacgaatc acggtacgac cttccgtcag aatgcggtcc atggtgtatt taccgataac
     7441 tccacgcagg gcgctgtcgg tagcctgacg caggctgtca tccgggctgg tcacgctata
     7501 cagatatttt tccggattgg tgacgcggta ctgcacgttc atctcaacgc gcactacgtt
     7561 ctcgtccgac gtcagcatca caccagaagc tgccagttca cgcacggctt ccacgttcac
     7621 cggtttgact tcgtcgataa acgtcggttt ccagttcaga cctggctcaa ccagatggct
     7681 gaatttacca aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat agaaaccact
     7741 ggccgcccag ataatgacaa tcgctgccgc tgcgatggta acgacacgac cgccaagctg
     7801 cgggcgcggg ccttgcgatg aactgccacc gccagatccg gtgcctttac cgccgcccag
     7861 accaccgagc tttttgctca gtttgcggaa gatatcatct aaatcaggtg gcccttgatc
     7921 gcgaccgcct ttgtttccat ttccctcaga gttgccgcca ggtttgctgc ttccccacgg
     7981 gtcgcggtct tgtccgttat taccgggctg attccacgcc atgtttgtgc tccatatttg
     8041 ttatgcggtg atccctgttg tctttcgacg ccagaggata tgactccacg cttcagacgc
     8101 tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg tttacagagg cgacgccagt
     8161 caacgatcgg catacgaact tgcagactta cgctgccgtc ctcctccatc cactcttttt
     8221 ctattgcctg aagctgataa aaacgacttc tcagacgccc ttcctgcggt ggcagacgca
     8281 atgtatgctg cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa agctgtggta
     8341 tccccgctcc ggtctgtgcg gaaagccaga cacggatcgg tttgttctct tcgtcccgat
     8401 caatacgcgg ttcgaaatct tccagcatat cgatcttgtt catcaccagc agggttggga
     8461 tctcgtgagc gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt tcttgtacac
     8521 gcacatccgc cgcatcaatc acatgcagca gcaatgtggc ttgccgcgtc tcttgtaacg
     8581 tggctttaaa tgctgccacc agatcgtgcg gcaggtggcg aataaaccct acggtatctg
     8641 caagtacggt ttcaccgaca tctgcaacgt caatacgccg caacgtcggg tcgagggtgg
     8701 caaacaactg gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa agggtagatt
     8761 taccggcgtt ggtatatccc accagcgaaa cagtaggaac gtcggctttg atacgcgatt
     8821 gccgcccctg ctcacgctgc ttttcaactc tttccaggcg cgactgtatc tgcacgatgc
     8881 gattacgcaa caaacgacgg tcggtttcga gctgggtttc acccggacca cgcaaaccaa
     8941 tcccgccttt ctgtctttca aggtgggtcc agccacgcac caggcgtgta gccagatggc
     9001 gcagctgcgc cagctcaacc tgcaacttac cctcatgggt acgcgcacgt tgggcgaaaa
     9061 tatctaaaat aaggccggtg cggtcgataa cacgacactc gcacaaacgc tccaggttac
     9121 gctcttgcgc cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc gtcgctttga
     9181 cagcttccgc aatttcaact gctttacctt cacctacaaa atactttggg tgcggcgctt
     9241 tacggctacc ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc agagattcaa
     9301 actcctggag gtcttccata tctttgtctt gcgtaaaata gatgtgtacc agtaccgcct
     9361 gctcaccagc atcataacgg tcaaacaagc gtataaccct ctaaatagat cagcggggaa
     9421 cgcaggatcg ctggctcccc gtgtaaaaaa aacagcccga aaccttattc ggtttcttcg
     9481 ctgtcctgtt gcgcggaagt attctgcgcg ctgctaccat gatggtagtt actgctggta
     9541 ccgccaccgg cgttgttact gtgatgagaa accgggcgag acgggacaac agtagaaatc
     9601 gcgtgcttgt aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa ctgatcaaaa
     9661 gactcgattt gcccttgcag cttaatacca ttcaccaaat aaatagaaac tggaacacgt
     9721 tcccgacgca gtgcgttcag gaacggatct tgtaaagatt gccccttagc cattctctct
     9781 tttccttata tgcttatttg tactttgaac ctttcgattc tgaaaaaatt gcgcacgata
     9841 cgtctcaatt gtacacattc agcctgcgat agcaccaaca acctgtaata cttcgtcacg
     9901 cgcctgttct ggtttttcac tgtcaagcca gtgaacccct tcccaaccac gcagccaggt
     9961 tatctgccgc ttcgccaact gtctcgtggc gcaaacacct cgataaacca tttcatcgta
    10021 tgagatttcg cctccaaggt aagaccacat ctggcgataa cccacgcaac gaatggaagg
    10081 caagtccgta tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt caaaacctga
    10141 agccaacatc tgatgaaaac gctgctcaat tcgttgatgg agcagttcac ggctcgccgg
    10201 ggcgatggcg aactgatgca cctgatacgg tagagcgtct cctgacgttt gcgtcagttc
    10261 cgttaaagtt ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc tttgtggatc
    10321 atttggatga atccttgctg ccgcaaccgg atcgatctcc tgaagttgac gatgcaatga
    10381 ctcccaacct tgctctgccg cctgttgctc aattctggcc cgtacttccg ggtctgccga
    10441 cggtagcggc gacaaccctt ccagcaatgc cttgaaatac aacatcgtac cgcccactaa
    10501 cagtgggatc cgccccgccg cggtgatatc ggccatttcc gccagcgcat cgcggcgaaa
    10561 atcagcagcc gagtaagcct gcgacggatc gcgaatatcc agcaatcggt gcggcgcggc
    10621 gagtaactct tcagcgttcg gcttcgccgt cccgatatcc atccctttgt aaataagggc
    10681 agaatcaacg cttatcaact ctactggtaa aattttacgc agctcaatgg ctaacgccgt
    10741 tttaccggag gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg ccttactgat
    10801 atcactcatc tttcagggct tttatcgccg gatgtaaatc aacagattgt aacagaccac
    10861 ccggcggcgt tttcacaagt tgcggacata accgttccac gtccgccagc agggttatgg
    10921 cctgtgccat tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc cactgcgcaa
    10981 tattgccagg ttcgaatacg gactgcttcg ctaggtagcc tatcagttca ggaatcaaga
    11041 tttgtaaatt ttgttggcgt aagggtaaag gcacggccct gatggtcaca tgctgtgcat
    11101 ctgactggaa atcaataccc aattccgcca gggcagactg cgctttttct aatgccgatt
    11161 tttcttcgcc agaaactttt agccgcaacg gaatcagcaa cggctgggcg caaacgggcg
    11221 cttcacccgg cgtcagttgt acctgacgca gccaacgttc tgccactggc aaggctaaaa
    11281 gtgaaatgtt gccgtcgcgc tccagcaacg cacagtcgga atggacgata gtcagtaccc
    11341 gaccaaaact ctgactgttc gccgcaagtg caggttcctg cggttccggc gcttttggtt
    11401 tttgcatcgg cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc tgtttctggt
    11461 aacctggctg cgcattcggc cagggggcag ccggacgact acctgatgct ggcgcaggag
    11521 tgtagcgcgg agctactggc tcacgaactg ccggttcagc aaagtgattg cgccccgccg
    11581 ccacgcggtt ttccggaatg ggacgcggtg caggttgggg ttcatcgtcc agcggtagcg
    11641 gcgtttccag ttgctgttgt agcacgctca gcacgccctg atagataaag tcatgcacca
    11701 gacgcgactg atgaaaacgc acttcgtgtt tggcggggtg cacgttgacg tccacctgat
    11761 gcgggtcgat ctccagatac aacacaaatg caggttgctg atcggccccc agtttgtctt
    11821 cacaggcctg gcggatcgcg tgattgatca ggcgatcgcg catcatgcga ccattcacgt
    11881 agcaatactg aatttccgcc agtgcgggcg tggtgtgatt tggatcggcc acccagccgc
    11941 gcagcgtgag atcgccgtgt tgccattcaa tcgccagcgc ttgttcgaga aaagcggtgc
    12001 cacaaatcgc gcctaagcgc cgttcttttt gcccgccttc cggcaccgcg cggtactggc
    12061 gtacgatttt gccgttatgc gacaggttga tcgtgacgtc gaaacgcgcc agtgcgatgc
    12121 ggcggatgat ttcatcgata tggttaaatt cggttttctc ggtgcgcagg aatttgcgcc
    12181 gcgccggggt gttgtagaac aggtccagta cctccagcgt cgttcccacc ggatgcgccg
    12241 ccggtttaac cgtcacgtcc atatcgcgcc cttcggcata ggcctgccag gcttcctgct
    12301 gttctgcggt gcgtgaagtg agcgtcaggc gggaaaccga actgatactc gccagcgcct
    12361 caccgcgaaa gcccaggctg ataatggctt cgagatcgtc cagagaggcg attttactgg
    12421 tggcatgacg cgccagcgcc agcgccagct cgtctttttt gataccacag ccgttatcac
    12481 gaatgcggat aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc gtcgcacctg
    12541 catcgaggct gttttccacc agttctttga ctaccgacgc aggtcgctcg accacctcac
    12601 ctgcggcaat ctggttcgcc agttgtggcg gtaagacctg aattggcatc aatcgtcctt
    12661 agtttggcag cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc gttgcaccct
    12721 gcggcgcaga ttgcatcgga tgcgcaagga aataattgcg caggcctttg taaatggctt
    12781 ctgccagctg ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg ttgttgctga
    12841 taaaaccggt ttcgaccagt actgatggga tatccggcga gcgcagaacg ccaaggctgg
    12901 cgtgttctgg tcgacgttta tgtatttcgc caatgcgttg caactgactg atcatactgg
    12961 tcgctacatc ataccctacc cgctgggaat gaccgaactg taaatccagc accgcctggc
    13021 ttaaataggg gtcagactga ctgttcgcca gcacatcacc cgccccgccc agcagctccg
    13081 actgtttctc gtgctgctcc agccagctgg ccatttcgct gttggcgcga cggttagaga
    13141 gcacccatac ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg tgaatcgaca
    13201 cgaggaaatt ggcgttttgc ttacgtgcca catcgctgcg ccccatcacc gagataaagt
    13261 aatccccgtc acgggttaaa acgcctttaa acatcggatc gtcattgagc aaagtacgca
    13321 atttacgcgc gatggcgatg gtgacatttt tctcccgcgt accaccgggg ccgatagcgc
    13381 cagggtcctg accgccgtgt ccggcatcaa tagcgatgat aattttatcg ccattgttag
    13441 ccgtcgcgcg cgctgccgga cgcgttaccg tattactgct gataacaccc gtagtgcggt
    13501 tactttccgt tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg acaaccgtag
    13561 gcgtttcaac gcgtttcaca accacaggcg gcggaggagg cggtggcggc gcatcggcgt
    13621 taatcgtaaa gacgacagtg taattgctgc cattctgccg cttcaccgct tcggttttac
    13681 cattttcggt aagatcgacc accagccgta gcgtttgtgc atctttaggc gttccggagc
    13741 gaatcgcctt caccagatta ttaccgctga acaacaacgg cagtccctga atcacgcccg
    13801 tttgtttgat atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca taatcaggat
    13861 cgccaataaa actcaacgtt atccgcgcct gttggttgcc gttagaaacc tgaatatcag
    13921 agagcgtcgc ggcacccacc tgcgcgcaca gcagcagcaa cgtcgctacc aaccaatttc
    13981 tgatgcgata catcatccca ccacctttca aaggttaacc ggctaaacgt gccagcaaca
    14041 attcacccgc agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc tgataatcaa
    14101 tgtgtatttc gacatccggg tcaggaagaa cacctgtacc ttgttgtggc cactccacca
    14161 ggcagatggc atcgttggca aaataatcgc ggatccccat aaactccagc tcctcgggat
    14221 cggcaaggcg gtacaaatca aagtgataaa ccattaaatt gtcgagcgta tagggttcga
    14281 ccagcgtata agtggggctt ttgacattac cctgatggcc caaagcctgt aaaaagcccc
    14341 ggctaaaggt ggttttacct gcgcctaaat cgccatacag atagattacg gttgcgccat
    14401 cgcaggcttt cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca gggagcggaa
    14461 ttactcgatt catcatggtt tttatcagtc acttccgggt taacaatacg ctgtagcgtg
    14521 gaaaagagat cggttgccag catcccgcgc gttccaaaac gcgccgccag tacgtcagct
    14581 gccgcaccgt gcgcgacaca gcctgcacag gctgcatcat acggcgacag tttttgccca
    14641 agcaatgcgc caataatacc agagagcaca tcgcccatgc cgccgcttgc catgcctgca
    14701 tttccggcat caataatgcc taaagcgtca ggatgggcgg cgaccacggt tccggcacct
    14761 ttcagcaccg ctacgccgcc ataacgttgt accagacgtt tggcgcaatg taagcggtca
    14821 ctttcaattt cagcgacgga acagcctaac aaccgtgcgg cctcgccagg atgcggcgtg
    14881 atcacgcgat tgtgacgctt atcgggattg attgccagca ggttcaatgc atcggcatcc
    14941 cacaacatcg gtttgcgaaa attctcaact ttttgcaggg cttttttccc ccactcttgc
    15001 tggcccagac cgggaccaat caccaccaca tcggcccatt ccaggctttc ggtaagagag
    15061 tccatagtca gttcatgcac catcaattct ggtcgtgcag tcagcagcgg cgcaatgttc
    15121 tcactgcggg tcagtactcg gactaaacca gcaccagcac gtagcgccgc ttcccccgtc
    15181 atacgaatag cccccgccgt gccgtgatcg ccaccgataa tcaccagccg cccgtgatcg
    15241 cctttatgcg aagtcgggcg acgcggtttc agccagtgag aaagttgttc tgccgaaaac
    15301 cgctgaattt tcgtctcctg acctgccagc cagctatcca gccccagtga gtcaaaatgc
    15361 agttgtcctg taacatcccg cgcttttcca gtgagcaagc ctggtttcag cgcaataaaa
    15421 gtgatggtgt gatcggcgtt gatcactgcg cctggcgtag cgccggtttc agccagcagg
    15481 ccggaaggga tatcaaccgc cacaatcggc gcaggatggg aattagcgtg gtcgattaac
    15541 tggctaatgg attcgcgggg tgcttgctgc aagccggtac cgagcagcgc atcaacaatc
    15601 agagctaccg actcgggcca gacaatattc gaagcatgga tctcgcctcc cgcgtttaac
    15661 catgcctcgc gtgccagcgc ggcctcttcc ggcaacggtt tatcgctctc ctgggccaac
    15721 aacgtgacct caatgccgac cgctttggcc agtcgcgcga ccacgtagcc atcgccgccg
    15781 ttattaccat gaccgcacaa caccagccag tggcgggcgt caggatacgc cgaacgacac
    15841 acctggaatg cggcctcgcc agcgcgaagc atcagctcat agagtgtgag ccccagcgca
    15901 tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt gccagacggt gtgtggtata
    15961 cttacggggt ttttcttcat tgtatggtcc gtcatgtcag agcccctcga tctcaatcag
    16021 ttagcgcaaa aaattaaaca gtgggggctg gaactgggct ttcagcaggt aggtattacc
    16081 gataccgatc tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa acaataccac
    16141 ggcgaaatgg actggatggc acgtcacggt atgctgcgcg ctcgccccca tgagttattg
    16201 cccggtacgc tgcgcgtgat cagcgtgcgg atgaattacc ttcctgctaa cgccgcattt
    16261 gccagcacgc tgaaaaaccc caaactcggc tatgttagcc gttatgcgct gggccgtgac
    16321 tatcacaaac gtctgcgcaa ccgactcaaa aagctgggcg agatgattca gcaacattgt
    16381 gtttcgctga attttagacc gtttgtcgat tctgcgccta ttctcgagcg cccgttagct
    16441 gaaaaagctg ggctcggctg gacaggtaag cactcactta tcctcaatcg cgaggccggt
    16501 tcgttcttct ttttaggcga attgctggtc gatattccgc tgcccgtgga tcaaccagtc
    16561 gaggaaggat gcggcaaatg cgtggcctgt atgacgattt gcccgaccgg tgccatcgtc
    16621 gagccatata ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga actggaaggg
    16681 gcgatcccgg aagagttgcg accgttaatg ggaaaccgta tttacggttg cgatgactgc
    16741 cagcttatct gcccgtggaa tcgctattca caactcacta cagaagacga tttcagcccg
    16801 cgtaagccgc tacacgcacc ggaactcatt gagttattcg cctggagcga agagaagttt
    16861 ttaaaagtca cggaaggttc ggcgattcgc cgtataggtc acctgcgctg gctgcgtaat
    16921 atcgccgtgg cattaggcaa tgcgccctgg gatgaaacga ttctggcggc gctcgagagt
    16981 cgcaaaggtg agcacccact tcttgatgag cacatagcgt gggcgatggc gcagcaaatc
    17041 gagaggcgaa atgcgtgcat agtcgaagtg caattgccga aaaaacagcg tctggttcgg
    17101 gtaattgaaa aagggttacc gcgtgacgcc tgattcatcc acagcttgtg aataaaaata
    17161 aaaacgcatt gcaattcaag acgaataaaa tcgtcaagtg atcgactcga caaaacgaaa
    17221 tcttaatttt taaattatct atcaataggt taaaaaaata ctattcaccg tgcgaagttt
    17281 ctttgaaatt aagattacgg cctgaggctg tggataactc tgtttacaag agtttgtcag
    17341 agacaacaaa aaccagcaat aacgcatcgc gtcgctgtgg atattttatt gagagaaga
//
LOCUS       DABSYT020000092        17324 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.092, whole genome shotgun sequence.
ACCESSION   DABSYT020000092 DABSYT020000000
VERSION     DABSYT020000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.092"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..451)
                     /locus_tag="ID725_RS18440"
                     /old_locus_tag="pgaptmp_003688"
     CDS             complement(<1..451)
                     /locus_tag="ID725_RS18440"
                     /old_locus_tag="pgaptmp_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HGV7852194.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     TAAAEKAALKLIAEKVKKGYVEQVDANAHSHDASAKKVLVTEEKASINRQAATNGLPW
                     LADNDPIILPPNIARHALSHRLWPGEPVKKPTKPEKQNLISRLANRTY"
     gene            complement(464..4273)
                     /locus_tag="ID725_RS18445"
                     /old_locus_tag="pgaptmp_003689"
                     /pseudo
     CDS             complement(464..4273)
                     /locus_tag="ID725_RS18445"
                     /old_locus_tag="pgaptmp_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            complement(4414..6447)
                     /gene="metG"
                     /locus_tag="ID725_RS18450"
                     /old_locus_tag="pgaptmp_003690"
     CDS             complement(4414..6447)
                     /gene="metG"
                     /locus_tag="ID725_RS18450"
                     /old_locus_tag="pgaptmp_003690"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGV7852195.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            6579..7688
                     /gene="apbC"
                     /locus_tag="ID725_RS18455"
                     /old_locus_tag="pgaptmp_003691"
     CDS             6579..7688
                     /gene="apbC"
                     /locus_tag="ID725_RS18455"
                     /old_locus_tag="pgaptmp_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGV7852196.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            7950..8231
                     /locus_tag="ID725_RS18460"
                     /old_locus_tag="pgaptmp_003692"
     CDS             7950..8231
                     /locus_tag="ID725_RS18460"
                     /old_locus_tag="pgaptmp_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HGV7852197.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            8523..9065
                     /locus_tag="ID725_RS18465"
                     /old_locus_tag="pgaptmp_003693"
     CDS             8523..9065
                     /locus_tag="ID725_RS18465"
                     /old_locus_tag="pgaptmp_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7852198.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
     gene            9153..9827
                     /locus_tag="ID725_RS18470"
                     /old_locus_tag="pgaptmp_003694"
     CDS             9153..9827
                     /locus_tag="ID725_RS18470"
                     /old_locus_tag="pgaptmp_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGV7852199.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMPNKLPSNKES
                     IFYLNVLDIPPNSPEDEGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSGNN
                     VVIKNDSPNWVTISDVKANNVKVNYETIMIAPQESQNVSEKNNNANGWQLTIIDDHGN
                     YISDKI"
     gene            9843..12323
                     /locus_tag="ID725_RS18475"
                     /old_locus_tag="pgaptmp_003695"
     CDS             9843..12323
                     /locus_tag="ID725_RS18475"
                     /old_locus_tag="pgaptmp_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGV7852200.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNTLYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
     gene            12334..13368
                     /locus_tag="ID725_RS18480"
                     /old_locus_tag="pgaptmp_003696"
     CDS             12334..13368
                     /locus_tag="ID725_RS18480"
                     /old_locus_tag="pgaptmp_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7852201.1"
                     /translation="MMFRNRILLIFILWANFTWAGCRTTASLNITDGINVGEILANET
                     SFSKSVVFTGISCDTSTDKIVYKNIQSDWVEVGPFGNGEKLKVKIESLGKTSDTIGKS
                     SNAQAVLPYVVKIARGTPDFTGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKF
                     NWCVNYLTSKLAGDTFTLGLNISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANS
                     KEGTITLKCDNLFGDKKQASRNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPP
                     KGTEAAIKISANGDQGAATSLWKTDKPGVSLNSNIINIPVMASYYVYDEKKVKSGALE
                     ATALINVKYD"
     gene            complement(13450..13788)
                     /gene="rcnB"
                     /locus_tag="ID725_RS18485"
                     /old_locus_tag="pgaptmp_003697"
     CDS             complement(13450..13788)
                     /gene="rcnB"
                     /locus_tag="ID725_RS18485"
                     /old_locus_tag="pgaptmp_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGV7852202.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(14006..14869)
                     /locus_tag="ID725_RS18490"
                     /old_locus_tag="pgaptmp_003698"
     CDS             complement(14006..14869)
                     /locus_tag="ID725_RS18490"
                     /old_locus_tag="pgaptmp_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGV7852203.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHDHDHEHHHHHEHGDNE
                     EYQDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTL
                     GATLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVY
                     MGVHGFMGIMR"
     gene            14990..15262
                     /gene="rcnR"
                     /locus_tag="ID725_RS18495"
                     /old_locus_tag="pgaptmp_003699"
     CDS             14990..15262
                     /gene="rcnR"
                     /locus_tag="ID725_RS18495"
                     /old_locus_tag="pgaptmp_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGV7852204.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(15372..15686)
                     /locus_tag="ID725_RS18500"
                     /old_locus_tag="pgaptmp_003700"
     CDS             complement(15372..15686)
                     /locus_tag="ID725_RS18500"
                     /old_locus_tag="pgaptmp_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HGV7852205.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(15696..16043)
                     /locus_tag="ID725_RS18505"
                     /old_locus_tag="pgaptmp_003701"
     CDS             complement(15696..16043)
                     /locus_tag="ID725_RS18505"
                     /old_locus_tag="pgaptmp_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852206.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
     gene            complement(17094..>17324)
                     /locus_tag="ID725_RS18510"
                     /old_locus_tag="pgaptmp_003702"
     CDS             complement(17094..>17324)
                     /locus_tag="ID725_RS18510"
                     /old_locus_tag="pgaptmp_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HGV7852207.1"
                     /translation="TQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKEFCE
                     GVGLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT         4639 a         3697 c         4136 g         4852 t
ORIGIN      
        1 gataagtacg atttgccaat cgggaaatta aattttgttt ctcgggtttg gttggttttt
       61 ttactggctc tcccggccaa aggcggtggc tgagggcatg gcgggcaata ttcgggggaa
      121 gaataatggg atcgttgtct gccagccagg gtaaaccatt agttgctgcc tggcgattta
      181 ttgaggcttt ttcctctgtg accaggactt tctttgctga ggcatcatgt gagtgagcgt
      241 tagcgtctac ttgctccaca taccccttct tcaccttctc cgcaatcagc ttcagtgctg
      301 ctttttccgc cgccgcagta tctgcaaagc tttttacctg actttgcccg ttggtaccga
      361 ctttacccca gttaatatgc agctcgtttc cctgctgctc caccgcccag aatttatgtg
      421 atttttcgtc ctgataaata taggttttca ttgttaaatc cttttaatca aacagtgatt
      481 caatgtcgtt aatcagctcg ctggcggtga tgtcgtcgag catggagaaa gtgagtgagg
      541 attgtttacc gtagcgatca tctggcgagg tgatttttac gctttcaacc gggtgctggt
      601 cgccataccc cgtttcacca taaattaatg aaaaaggttc cgtttcaagt tcgagatcgc
      661 catgaggggt tgttttgcgc atcgcataaa cgctgccgct ttcttccaga cgttgccagc
      721 ctctgcgttc cagcccgaca atgcgtccgg cctggcaaag ccgtccttgc cagcgtgtca
      781 gttcgtgagt attacgttca ttatcagcaa ggtgatagct acctcgctcg agctgttgga
      841 aagggggaag cagttcgtaa tcagcgtaaa tttgcctgaa tgcagcggcg gattcagggg
      901 atatttccag cacatgcgga ataccgatgt ttcctgctgg cagcgtgaat aactcatcct
      961 gcgcatcgct gtaagtgctg tcttctgcca cgcgaaaaca ggtgataagg gcgttttcat
     1021 cgttataaac cccccataac agccgtcggg ttaagtgacg taccagcgga tgctccacca
     1081 gaaacaggcg gaactgctct gccgtccagc gtctgcgctg gcacatagcc tgttccagac
     1141 gatcaatctg ctggctggct atggcgcgta catctttttt caactgtttg aagagattaa
     1201 ccgcgtcagt tgcctgagtt ttttcatcgc tctggtttgg cttaggtaaa tctttcagga
     1261 ctttgccgtt tgcatcgcac actacaggtt ttaaggtttc gtcaaaacca acggtgaatt
     1321 tgcgggggcc gaaatccagt atcagcgagc cactgctatc aagccctaaa tctggagcca
     1381 ggcggtcttc cagttcagcc atagtcagcc cacggttttc agcaactatg ttgattttct
     1441 cgcgggcatg ttcctgtaat gctttgaatt taatttttcg cgcgatgcca ttaagcagca
     1501 ttagcgcaac atcgctgcca atatcagcta atacatccag cccggacacc gcccgtttat
     1561 gctgggattc cccaggccag gcgcggataa gcggggttaa tttacgcgcg gtgtcgtcat
     1621 tgcctaaaat cccaagcgaa gtaaatgccc agttttcttt tgcgggtgcg ccagcttctg
     1681 tccaggcggg atacagatcc cagccaaatt cagccagtga ttcgcgggtg aagatttctc
     1741 taatgatatc cagcccagcg taggcggtaa tgtcgcgagg aaagctcagc atagtgccga
     1801 ggtggcgcat agcgtcatct gacagaggca ggttgttact tttaagccgt ggtctgcgcc
     1861 acaaggtgaa ctgataaaag ccgggcagtg gggcgatttt agcgggatat tcttccagtg
     1921 ggtcgctatc gaacaatgtt gccagcgcag ctaaaacatc aggttgatta tatccttgcg
     1981 cgatttcctc aatcgtttct cgctggttta aattcaccag cagacgaagc gcctggcggg
     2041 cacaatcacg atcttttcct tttttgccca gggccacagg aagcagcccg gcggcggcat
     2101 ggcgtggata atcagttagc cagtttcgcg catcttcata agacattttt ttctgtaatt
     2161 gttgcgccat cggtaaggcc agttcactga caccgaggaa aaaggggaag aagcacagac
     2221 gtcgtggatc acgttgcagg caggccataa atgcaggtat ggcggcgcta ccaaaaattg
     2281 ccagaatatt tccctcacca gagtagttat cttctttgac tatctggtta agaaaagaaa
     2341 tggcctgttg accaggcagg taacagaggg caatgagatt ccagcttttg tatgcattgt
     2401 tatgataatg cacaaattca gcattgagtg ctggaaaatc gaagcgtagc agggcatcaa
     2461 cggcgctttg tggcgccgga attttttcgt atattttttc tccgctctcc cgccattttt
     2521 caaaaccaag atgccacagt atttgttgct ccggtgtaag cggtattaag tgtttctccc
     2581 agcttgcttt tttgatcggc ggcaggtcgg taaacaatgt ttgttttgtc gcctgctgtt
     2641 gggctatttg ctggaagtgg gtgacaaggt aagtacgggt cagttttttg gtaatctctg
     2701 gagtgacatc agaaacagaa ggaactggaa gcactggaag atcaaacaca ggcgtatttt
     2761 tctttttctc tttggtcatc cagggcggcg aaacaagcag ctccggcaac atatccacag
     2821 tggcatattc acattcttca gcgactgact gcggacgaca ttcagataag atgcctgccg
     2881 ctttagcatt aacccaggga atgacgtcgc atactaactc tggcgtggcg gtaatcagtt
     2941 tgacaagcgc tttacgccac tctttatctt catgtattgc cagcaaagta gcataagccg
     3001 caatagccgc agcgggatgc ttttgactgg ccttgcttaa gtattccagg tgctttttct
     3061 taccaattac accgaggatc agaatttgaa tcgcatccgg gtgattcgtt ttccatagcc
     3121 atttgacaag attttcagtc tcgctgatgc gttcgaagag ataagtggga ttgtcagaat
     3181 ggtcttccgt aaactcaaga agtatccggc ttacaccgcg caccccatta tttgccaaca
     3241 acattgccag ataagcgcga aaattacgaa aatcacgata caacggtggg aagtcgtatt
     3301 tttccactgt actcagagca gtatcatcgg ttatcaccag tcttaaccag gtcatcgccg
     3361 ggacgttttg gtctgcatag cgtagatcta tagcatgggc gatatccggg cgttcaggga
     3421 gcagtaacgc cgcaaaaggt tgccgtgcgg gggatagcgt cggaatggcg gtgagtactt
     3481 tatctacaca gcgttgccac tcatcttctg aagcgagtga aagatgatga catagacgtt
     3541 gatgctaact aggtagcgat tctgcttcgt gattaggtgg aatgatggtg gttactgcgc
     3601 cttttactgg tttatatttg agttgaaggg caaaaaggac gatttcaacg gcgctttcca
     3661 gaccacagcg aatgactaag tcatcgacca gttcatctgc agagtaagag ttccaaaaac
     3721 tccagagcaa tgctgcatca gattgcgcac tccctgtctg ttgattatcc agaattcgcc
     3781 tttgtaactc agcaaaagcc tgctgccagt tttcaggata agagcggtaa ttgttgtcgg
     3841 gatcatatgc cctggtgtta tctgcaatac gcttccatat ctcaccgtct ttgcgcagat
     3901 aattgatctc tctggggcga gagcgatgag gaaaagcaaa tcggttaata tcgtcagtta
     3961 ccggaataac tgcatcatca gccagccacg ggcgttggtt tttactctca ggggaagttt
     4021 caacttcagg agtggctttg gtgatggggg ggatatgcac gtttgctgaa gcattttcca
     4081 catagccttt tttcgttttc tccgcgataa gcttcagttt cgctttttcc gctgctgcaa
     4141 catccgcaaa actttttatc tggctttgcc cgttagtgcc gactttgccc cagttgatat
     4201 gcagctcgtt tccctgctgc tcaaccgccc agaatttatg tgatttttcg tcctgataga
     4261 taaagtgtct cattctgcat ccctgtaatt ttatccagtt gaatgcagat gctaccagta
     4321 tttatacggg ttagagagag acaaaatcgt aagaaaacaa ctttaggaat ttataaagaa
     4381 aaggcgctgt cgatgcagcg ccttgaaggg ggattatttc acctgatgac ccggtttagc
     4441 accggcatcc gggcttagca ggaaaatatc tttcccgcca ggaccggcag ccatcaccat
     4501 gccttcagag ataccgaagc gcattttacg tggtgccagg ttagccacca taatggtgtg
     4561 acgaccaatc agtgcctgcg gatctgggta agcagaacga atgccggaga agacattgcg
     4621 tttttcaccg ccgagatcca gcgtcaggcg tagcagtttg tcagaacctt caacaaactc
     4681 tgcgttttca atcagcgcca cgcgcaggtc aactttggcg aagtcgtcaa aggtgatggt
     4741 ttcctgaatc ggatcatctg ccagtgggcc agtaactggc gcggcagctg ctttcacttc
     4801 ttctttagac gcttccacca gtgcttcaac ctgcttcata tcgatgcggt tatacagcgc
     4861 cttgaacgga ttcactttgt ggcccagcag cggttgctgg ataccatccc aggtcagttc
     4921 tgtattgaga aatgcttctg cgcgctcggt cagtttcggc agtaccggct tcaggtaagt
     4981 catcagcacg cggaacaggt tgatgcccat tgagcaaatc gcctgcaggt cagcatcgcg
     5041 gccttcctgt tttgccacca cccacggagc ctgttcatcg acatagcggt tagccagatc
     5101 agccagcgcc atgatttcgc gcactgcttt accaaattcg cggctttccc acgcttcacc
     5161 aatcacttca gcggcatcag tgaaggtttt gtacaactgc gggtcagcca gttcgcttgc
     5221 cagcacgccg tcaaaacgct tgttgataaa gcccgcatta cgagaggcca ggttaaccac
     5281 tttgttaacg atatcggcat tcacgcgctg aacgaaatct tccaggttga gatcgatatc
     5341 atcaatgcgc gaagagagtt tcgcagtgta gtagtaacgc aggctgtcgg cgtcaaaatg
     5401 attcagccag gtgctggctt taataaaggt accgcgagac ttagacatct tcgcgccgtt
     5461 caccgtcaca tagccatgaa caaacaggtt ggtcggcttg cggaagttgc tgccttccag
     5521 cattgctggc cagaacagac tgtggaagta aacaatatct ttaccgatga agtggtacag
     5581 ctcggcggtg gagtctttct tccagtattc atcgaagctt acgctgtcgc cgcgcttgtc
     5641 gcacagattc ttgaaagaac ccatatagcc aatcggcgca tccagccaga cgtagaaata
     5701 tttacccggc gcgttcggaa tttcaaaacc gaagtacggc gcgtcgcggg agatatccca
     5761 ctgttgcaga ccggattcaa accactcctg cattttattt gccacctgct cctgcaacgc
     5821 accgctgcgg gtccatgcct gcaacatttc gctgaaagag ggcagatcaa agaagaagtg
     5881 ttcagaatca cgcattaccg gggtagcgcc agaaaccacc gatttcggct cgatcagttc
     5941 agtcgggctg taggtcgcgc cgcagacttc gcagttatcg ccgtattgat ccggggattt
     6001 acatttcggg caggtgcctt tcacaaaacg gtccggcagg aacatgcctt tttccggatc
     6061 gtacagctga gagatggtgc ggtttttaat aaaaccgttt tctttcaagc gagtatagat
     6121 aagctcagac aactggcggt tctcttcgct gtgcgtcgag tgatagttgt catagctgat
     6181 gttaaagcct gcgaaatcag tctgatgctc ctgactcatt tcgccaatca tctgctccgg
     6241 ggtgatacca agctgctgag ctttcagcat gatcggtgta ccgtgggcat cgtcggcgca
     6301 gatgaagttg acctcgtggc cgcgcattcg ctggtaacgg acccagacat cagcctggat
     6361 gtgctccagc atatggccga ggtggattga gccgttagcg tacggcagtg cgcacgtcac
     6421 cagaattttc ttcgcgactt gagtcatagt aggcattact tcttaatagt gaaaaggggt
     6481 tagatagtac caaatgggaa aatgttaagt aagtacttct ctatattctt caggtaaact
     6541 atcagctgat ggtttatcta tacaaaaaag gagtcgggat gaacgaacaa tcccaggcca
     6601 aatcaccaga agccttgcgc gcaatggtcg ccgggacact ggccaatttt cagcacccaa
     6661 ccctgaagca taacctcacc acgctgaaag cgttgcacca tgtcgcctgg atggacgaca
     6721 cgttgcatgt ggaactggtt atgccattcg tctggcatag cgcgtttgaa gagttaaaag
     6781 agcaatgtag cgccgaactg ctgcgtatca ccggggcgaa agctatcgac tggaaactgt
     6841 cgcacaatat cgccacctta aaacgcgtga agaatcagcc gggtattaat ggcgtgaaga
     6901 acatcattgc ggtcagttcc ggtaaaggcg gcgtgggtaa atcctccacg gcggtaaacc
     6961 tggcgctggc actggcggcg gaaggggcga aagtcggcat tctggacgcc gatatctacg
     7021 gcccatcaat tcccaccatg ctgggcgcag aaaatcagcg tccaacttcg ccggacggta
     7081 cccatatggc accgattatg tctcatggcc tggcaaccaa ttcaattggt tatctggtca
     7141 ctgacgacaa tgcgatggtg tggcgtggac cgatggcgag caaggcgtta atgcagatgt
     7201 tgcaggaaac cttgtggccg gatctcgact atctggtgct tgatatgccg ccaggcaccg
     7261 gtgacattca attgacgctg gcgcaaaaca ttccagtaac tggtgcagtc gtggtcacca
     7321 ccccgcagga catcgcgttg atcgatgcga aaaaaggcat tgtgatgttc gagaaagtcg
     7381 aggttccggt gctcggtatt gtcgagaaca tgagtgtgca tatttgcagt aactgtggtc
     7441 accatgaacc gattttcggt accggtggtg cagagaaact ggctgaaaaa taccatacac
     7501 agctgctggg gcagatgccg ttacacatct ccttacgtga ggatctcgat aaaggcacgc
     7561 caacggttat cagccgtccg gagagcgaat ttaccgctat ctaccgccag ctggctgacc
     7621 gcgttgcagc tcagctctac tggcagggtg aagtcattcc aggcgagatt tccttccgcg
     7681 cggtctaacg cctattaacc attctggtta ttcttcatac accgcagaga tgttatattg
     7741 atgcggtgta tttgtttttg ccgcattaat gaacacttat ttaatactgc gaatattcag
     7801 tggatcttag tccgaaactg tacaaaggcg attgattgtg caaccactgt ctctttatca
     7861 tccctcgtgg ctgtgtagcg gatgtacagc tatagtgtgt tatgcactca atctacccgg
     7921 cataaccttt actacaggga taacgttcaa tgaataagta ttggttgtca ggagctgttt
     7981 ttcttgctta tggactagca tcacccgcgt tctcttctga tactacaaca ttaaccatca
     8041 atggcagaat tagttcccct acatgcagta tggatgttgt taataatcat cttcagcaac
     8101 gttgtgggca gttgttacaa cgagttgaaa ccaactatcg tgcttcttct actgcaaaag
     8161 gcgttacgac agaagtggta gctgtgggta ataacgacaa acgcaaaatc gtgttaaatc
     8221 gctacgatta atttgccaac accgttttaa gcatatttaa aaagccaaaa tcattcctat
     8281 gaatgatttt ggctttttta ttcatcccat aaatgttatt tacggtaatt tacggtaatt
     8341 aatcagtttc aaaaatgttt gttatagcga attatttgtt gtatttatat tgcattagtt
     8401 aatttgtttt aaatcaacaa aacaatatgt ttacattgcg gcaacctttt tgatgtgaga
     8461 aatttgtttc caagagatac agactcttaa caaaacaaaa taaacttatt aaggaatgca
     8521 tcatgaaaca ttcaattatt gctgtcgctg tcttatcttc tgtatttatg agtgctggta
     8581 ttttcgctgc tgaagtagat accggaactt taaccattaa aggtaatatt gcagaatcac
     8641 catgtacatt tgtcgctggc ggtgattcag tcagtattaa tatgccgact gtacccacca
     8701 ctgtatttga tggtaaagcg aaatattcaa cttatgatgg cgcggttggt gtaaccagca
     8761 gtatgctgaa aattacttgt ccgaagacta ttgatggcgt aaaactttcg ttgatcacca
     8821 acgagaaaat tcttggtaat gataaggcaa tatccagtag taatgacaca gttggatact
     8881 atctctattt gggagatagc agtgacgtac tggatatttc cgcaccattt aatattgaaa
     8941 gttataaaac tgctgatgga caatatgcaa taccgtttaa ggcaaaatat ctgaagttga
     9001 ctgacagtgg tgttgcatct ggtacagtta actcttcatt ggttatgcgt gttgcccagg
     9061 attaaatatt taaaataatc atcttgtttt ttcagtaagt taaaatggcc gccatattca
     9121 tggcggccag cataaatata agaggcatta agatgaaagg gttattatct ttactcatct
     9181 cttctatgat attccccgtg catgccggaa tggttatttt cgggacccgt atcatttacc
     9241 cggcagagca gaaagaggtc atggtacaac tgatgaatca gggaaaccgc tcttcgctga
     9301 tgcaggcgtg gattgatgat ggcgacacgt ctctgccgcc agaaaagatt caggttcctt
     9361 tcatgttaac gccaccagta gtgaaagtag cggccaattc tgggcaacaa ataaaaatta
     9421 aaatcatgcc taataaatta cccagtaata aggaaagtat tttttatctt aatgttctgg
     9481 atattccacc gaatagccca gaagatgaag gaaagaatgc gttaaaattt gccatgcaaa
     9541 acagaatcaa gttgttttac cgacctgctg gtattgctcc ggtaaataaa acgactttta
     9601 aaaaattacg agtgagtcgt agcggtaata acgtagtcat aaaaaatgac tcgccaaatt
     9661 gggtaacgat ttctgatgtt aaggctaata acgtcaaagt gaattatgaa acaatcatga
     9721 ttgcaccaca agaaagtcag aatgttagtg aaaaaaataa taatgcgaat ggctggcaac
     9781 tgaccattat cgatgatcac ggcaactata ttagtgacaa aatttaattg caggagctgc
     9841 ctatgttgag aatgaccccg cttgcatcag caatagtcgc gttattgctc ggcattgaag
     9901 cacatgcagc agaagaaacc tttgacaccc attttatgat gggaggaatg aaaggtgagc
     9961 aagtaacaaa tttgcgtctt gatgataatc agcctttacc tgggcagtac gacattgata
    10021 tttatgtcaa taaacaatgg cgtggaaaat atgagattat cgtcaaagat aacccacatg
    10081 aaacttgcct gacgcgtgaa attgtaaagc ggttaggtat aaatagcgat aattttgcac
    10141 gcgaaaatca gtgtttaaca tttgagcaac ttgttcaggg cggaagctac tcctgggata
    10201 ttgggatttt tcgtttggat ctcgctgttc cgcaagcctg ggttgaagag ctggaaaatg
    10261 gttacgttcc gccagaaaac tgggagcggg gaatcaatgc gttttatacg tcttattacg
    10321 taagtcagta ttacagtgac tataaagcct cgggcaatag taagagcact tatgtgcgtt
    10381 ttaacagtgg gctaaatttg ctgggatggc agctgcattc agatgccagt tttagcaaaa
    10441 cggataataa tcctggtgag tggaaaagca acacgctgta tctggagcat ggattttctc
    10501 aaattctcgg tacgctgcgt attggtgata tgtacacctc agccgatata tttgattccg
    10561 ttcgcttcac cggggttcgg ttatttcgtg atatgcagat gttgccaaac tcgaagcaaa
    10621 attttacccc acgggtacag gggattgccc agagtaacgc gttggtaact attgaacaga
    10681 acggttttgt cgtttatcag aaagaggttc caccaggtcc gttttcgatt agtgatttgc
    10741 agttagcggg cggaggggcg gatcttgatg ttagtgttaa ggaagccgat ggttctgtga
    10801 ctacgtatct ggtgccttat gcggcagtac ctaatatgct gcaacctggt gtatcaaaat
    10861 atgattttgc ggcaggccgt agtcatattg aaggtgcgag caagcaaagt gattttgtcc
    10921 aggcgggtta tcagtatggt tttaataatt tattgacgct gtatggcggc acgatggttg
    10981 ctaataatta ctatgccttt accctcggaa caggttggaa cacgcgtatt ggcgcaattt
    11041 cagtcgatgc aactaaatcg cacagtaagc aagacaatgg tgatgtgttt gacggacaaa
    11101 gttatcaaat tgcctataac aaatttttga gccaaacatc aacacgtttt ggtctggcgg
    11161 cctggcgtta ttcgtcgcgt gattaccgca catttaacga ttatgtgtgg gcaaataata
    11221 aagataatta tcgtcgtgat aaaaacgatg tctatgatat tgccgattat taccagaatg
    11281 attttggtcg caaaaatagc ttttccgcca atatgagtca gtcattgcca gaaggctggg
    11341 gatctgtgtc attaagtact ttatggcgtg attactgggg gcgaagcggc agtagtaaag
    11401 attatcaact gagttattcc aataactggc ggcggataag ctataccctc gcggcaagcc
    11461 aggcgtatga cgagaaccac gccgaagaga aacgctttaa tatttttata tcgattccct
    11521 ttgactgggg cgatgacgtt acgacgccgc gacggcaaat atatatgtct aactcaacga
    11581 cctttgatga tcagggtttt gcttcaaaca ataccggatt atcgggaacc gttgggaacc
    11641 gcgatcagtt taactatggt atcaacttga gccatcaaca tcagggaaat gaaacgacag
    11701 ctggggccaa tttgacctgg accgcaccgg ccgcaacagt gaatggcagc tatagtcagt
    11761 cgagtactta tcgacaggtc ggagccagtg tttcaggggg actggttgcc tggtctggtg
    11821 gcgttaatct ggcgaaccgt ctttccgaaa cgtttgctgt gatgcatgcg ccgggaatca
    11881 aagatgctta tgtcaatggg caaaaatatc gtacaacaaa ctgtaatggt gtggtggtgt
    11941 acgacggact gacaccttat cgggaaaatc acctgatgat ggatgtgtcg caaagcgata
    12001 gcgaaacaga attacgcggg aaccgtaaaa tgaccgcccc ttatcgcggc gcggtcgttc
    12061 tggttgattt tgataccgat cagcgtaagc cctggtttat aaaagcatta agatccgatg
    12121 gacagccatt aacgtttggt tatgaagtca atgatatgca tggtcataac attggtgttg
    12181 tcggccaggg cagtcagata tttattcgca ccaatgaaat accgccagcg gttaatgtgg
    12241 caattgataa gcaacaaggg ctttcatgca caatcacctt cggtaaagag attgatgaaa
    12301 gtaaaaatta tatctgtcgg tgagaggtga aatatgatgt tcagaaatag aatattacta
    12361 atatttatat tgtgggctaa ttttacctgg gctgggtgtc gtactactgc atcattaaat
    12421 attacagatg gtattaatgt tggggagatt ttagcgaatg aaacttcctt tagtaaaagt
    12481 gtcgtgttta ctgggatatc ttgtgatacg agcacggata aaatagttta taaaaatatc
    12541 caaagtgatt gggttgaagt tgggcctttt ggtaatggcg aaaaattaaa ggttaaaata
    12601 gagtctttag gtaaaaccag cgacacaatt gggaaatcca gcaatgcgca ggcagtatta
    12661 ccttatgtgg ttaaaatagc cagaggcaca cctgatttta ctggagaaag aaaatctacc
    12721 tggtttattt cagataccgt gattgcaaat attggcggtg agtcatcgtc atccatcgat
    12781 ttttggttgg gtatttgtaa ggcattgaag tttaactggt gtgtgaatta tctcaccagc
    12841 aaactggcgg gggatacatt tacgcttggg ttaaatattt cctattatcc taaaaatacg
    12901 acctgtaagc ctgaaaacac cgttataaaa gtagatgata tcgccttgtt ccagctcaga
    12961 aatcagggaa agattgcggc gaacagtaag gaaggaacaa ttacgttgaa atgtgataat
    13021 cttttcggcg acaaaaaaca agcatcgcgg aatatggttg tatatctttc tagcagtgac
    13081 ttagttaaag gaagtaatac tattttgcgt ggtaaaacag ataatggtgt agggtttgtg
    13141 ttggatctaa cagaaccacc aaaagggact gaggctgcca ttaaaatttc ggccaacggc
    13201 gatcagggcg cggcgacatc attatggaaa acagataaac caggagtttc attaaatagc
    13261 aacattatta atataccagt catggccagt tactatgtat atgatgaaaa aaaagttaaa
    13321 tctggcgcac tggaagcaac cgcattaatc aacgtgaaat acgattaata acaagcaaga
    13381 tgagcaagtg gctaataata agaaataata aggtgtctgc atttccccct ctctccgagg
    13441 gggctttttt tagcgatgat aaaaaatctc accgtcgtag gctttaatga ttttaccgtc
    13501 ggtgtcgctg atcaacacgt acgcgccacc catataggtc cagtgcgtcc cggcatccgg
    13561 cgcgggcagg ttacgcaact gccactgctt aatgttgtac tcatcggtac ggtacatctc
    13621 tggtacggtg tcccctggtt tgtaatgctt aaagtcagca atgaaactgc ttaactcata
    13681 cttagaaatt cccgaggtat tggtcgaacc cgcggtggct ggtgcggccc aggcggcact
    13741 ggtaaccagc aaaagcgcac ccagcaacat cttattttta atggtcataa actctccact
    13801 ttggggatct gtaatatcag tcgcatatct ttcctgccat acaatataac tgacaagcga
    13861 tattttgtta gtgatcccat gatttcgtaa aaagtgtaaa tgaaaggatg tagcaaaagc
    13921 atcctcattg agcgagaagt gagcggaata agctgattta agaatcatcc tgggctaaaa
    13981 ggatagcgaa gcccaggtga ttgttttatc tcataatacc cataaagcca tgcactccca
    14041 tatacacccc gactaaacca attaacagac tggaaaaata gggggcgcgt ttagcgagag
    14101 tgttaaatcc gctccagcgt tttgcaactt gttgaacact gattgccgct ccaacgccta
    14161 ctgtaaccag cgttagtgcc agaccgatgc tgaaactgac gaccagtgtt gcgcccagtg
    14221 tcagggcttt caactgaatg caaatcaaca gcacggtaat tgctgccggg cagggaataa
    14281 ggccacctgt taagccaaat aacaaaattt gccagttggt gacctctcta ccatcaaagc
    14341 gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactct tcgttgtcgc
    14401 catgttcatg atggtgatga tgttcgtggt cgtggtcgtg gtcgtggtcg tggtcgtggt
    14461 cgtggtcgtg gtcttgatga tgttcgtgat cgtggtgatg atgctcatgc atattctcca
    14521 gccagttgcg ttcgccgcgc caggtacgcc agaacatcca gaaagcggtg ctaataatga
    14581 tcactgcgga aatcaactgg agccacggtt ctgctgattg agcagtaaag cgcttgctga
    14641 tcaccatccc gccaaaggca atcaaccaga ccactgcggt atgcgaaatg gttgctgcca
    14701 gtccgagcat caccgcctgt ttaatggtgc ctttgatggc gatgataaac gccgccatca
    14761 tcgtttttga gtgccctggt tccaggccat gaagcgcacc aagtaagatg gcgctgggga
    14821 tgaagaacca ggcgtttcct tgctgaagaa gagttgtaaa ttcggtcatg ataataattc
    14881 ttagtattaa ttcggcaatc tgattctact cccccccagt acctgatact accccccagt
    14941 agattaatag tgctatgatt tttcatgttc ttgttaacca ggtgttgcca tgtctcatac
    15001 aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag attcagggcc aggtcgtggc
    15061 gctcaagaaa atgctcgacg agccgcacga atgcgctgca gttttacaac agattgctgc
    15121 tatccgtggc gcggtaaacg gtctgatgcg ggaagtgatt aaaggtcatc taacggaaca
    15181 tatcgttcac cagggggatg agctaaaacg tgaagaagat ctggatgttg ttctgaaggt
    15241 gctggattct tatatcaaat gaaagtgcct aatatggccg tagttgaggc aataaataga
    15301 gggacaagtg acgttaagct gaattagagt aaatggcgtg aggacacacg ccattcgagg
    15361 ttatagtttg attaattttc gaaaaattgc ttcacagccc agccaccatg accacctgga
    15421 gttatgaatg aaacaaaacc tttgtcatct acgctctgga tttctaccgt cattgattca
    15481 tctaacacgc gacatttatt ggatgtaatg agactcagca tagcctgttg gtcattgtcc
    15541 tggatgtaac tgatcatctc gtaaaagtca gttaactttt tacagttaaa tgtaccttct
    15601 ttaagtgttg cgctgtctcc gtgacttagt gcgaatgcat ttggcatcat tgccacaaag
    15661 atgacggtaa cgagtagttt tttcatttta gcctcttata attcagaaca ttcactgatg
    15721 ttgtaagtgc cttttctacc atcatcgttt acgagatgta gcacgaagat gatgggattg
    15781 gcatcaatga aagatacaga gacgttcccg ctttcggtgc tatacatctt tgcaatctgt
    15841 ttttcaccaa tatatgagag atcccaactc actccgttca agctaaaatg catctgtttg
    15901 ccgtcaatat cgatggagga ttgtgccgta acggttttcc cattgctgcc aatttgaccc
    15961 tgctgaagag catcacattt ataggagctt gcataagtag atagaggtag tagcagaagt
    16021 gcagcaaaaa ttttactctt catattttcc ctttgcttgg atttggatta cctattttgg
    16081 ggtagtcatt ataaaactat taattagtaa aacgaacaag ttttcatttg tttagcaatg
    16141 gtattgttta ggtttgaaat tattacgtat tttcatagca ataggttgga taaacagcag
    16201 ttcagggaac aagttgcact atctacatac ttttccgctt tccattgcat gttaggtaat
    16261 agatagcctt tgcccgtaag actaaaaata ttagaaatga ctcattcgaa taccttctat
    16321 tcaccttaat ataggtttga ttattgtaat cgtttaatgt gtcttatgat tttttcctac
    16381 ttttcatttg cataatatag ggggcacagc atgctcttga aattaattat taaaatattt
    16441 aaattttaca gcacacaact acttttcgtt gtcttatgtg ttgaacgtac ttcgagtata
    16501 ggtagggttg tatatatatt gcgtgggtag agttagaata gaggaagcat catttataga
    16561 atattcaaga acgttagcct gtgatcaata aataaaaata tcaaatggtg ttcaaacata
    16621 tttttctatg aaaaatccag ccatctatga tgaattttta gatccatttt aattaaagta
    16681 tgattatgaa ccaagattta gccaagaatg ggggtatagc gcgtatccaa tgacaccgga
    16741 aaacgcatct atgaagtcct tatttatctc tataagagga aatcaacact aataaaatgt
    16801 agtgatgtaa catatcgagc aggttgctct gatgctggtc ttcaactgtg gcttaaacag
    16861 ctatcaaact tcggtgttat tgagatgaaa gaattatcat tctcaactat caatctcaaa
    16921 aagctagaga aaatagacct taattattcc actctctaaa tttatctatt tatttaaagt
    16981 aaaaatgaaa atattatggt atcagctacg tggtggtaac tggtttttat gatcataggt
    17041 acatatacag ggaaccctgg aaaacgaaag taatatattg atgatttcgt ttgttataac
    17101 ttcgtggtat caagtccggc ggctttgagt cgctcggtaa gatttctcat tttgctgaac
    17161 tctgtagcaa agctggcttt aagtcctaca ccttcgcaaa actcttttgc cgtgacgttt
    17221 ttcagatggt cagcgtattt gatcatctgg aggtgcattt ccatcgtttg gctatttcgt
    17281 ggtgcgctgc taatggcttg cttaatgttc tcgtagatct gggt
//
LOCUS       DABSYT020000093        17298 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.093, whole genome shotgun sequence.
ACCESSION   DABSYT020000093 DABSYT020000000
VERSION     DABSYT020000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.093"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            119..1354
                     /gene="moeA"
                     /locus_tag="ID725_RS18515"
                     /old_locus_tag="pgaptmp_003703"
     CDS             119..1354
                     /gene="moeA"
                     /locus_tag="ID725_RS18515"
                     /old_locus_tag="pgaptmp_003703"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGV7852208.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            1354..2103
                     /gene="moeB"
                     /locus_tag="ID725_RS18520"
                     /old_locus_tag="pgaptmp_003704"
     CDS             1354..2103
                     /gene="moeB"
                     /locus_tag="ID725_RS18520"
                     /old_locus_tag="pgaptmp_003704"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGV7852209.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENVLTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2282..2944)
                     /gene="fsa"
                     /locus_tag="ID725_RS18525"
                     /old_locus_tag="pgaptmp_003705"
     CDS             complement(2282..2944)
                     /gene="fsa"
                     /locus_tag="ID725_RS18525"
                     /old_locus_tag="pgaptmp_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGV7852210.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            3075..3974
                     /gene="ybiY"
                     /locus_tag="ID725_RS18530"
                     /old_locus_tag="pgaptmp_003706"
     CDS             3075..3974
                     /gene="ybiY"
                     /locus_tag="ID725_RS18530"
                     /old_locus_tag="pgaptmp_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGV7852211.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3980..6411
                     /gene="ybiW"
                     /locus_tag="ID725_RS18535"
                     /old_locus_tag="pgaptmp_003707"
                     /pseudo
     CDS             3980..6411
                     /gene="ybiW"
                     /locus_tag="ID725_RS18535"
                     /old_locus_tag="pgaptmp_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            6557..7372
                     /gene="ybiV"
                     /locus_tag="ID725_RS18540"
                     /old_locus_tag="pgaptmp_003708"
     CDS             6557..7372
                     /gene="ybiV"
                     /locus_tag="ID725_RS18540"
                     /old_locus_tag="pgaptmp_003708"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGV7852212.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7524..8789
                     /gene="ybiU"
                     /locus_tag="ID725_RS18545"
                     /old_locus_tag="pgaptmp_003709"
     CDS             7524..8789
                     /gene="ybiU"
                     /locus_tag="ID725_RS18545"
                     /old_locus_tag="pgaptmp_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGV7852213.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(8957..10549)
                     /gene="ybiT"
                     /locus_tag="ID725_RS18550"
                     /old_locus_tag="pgaptmp_003710"
     CDS             complement(8957..10549)
                     /gene="ybiT"
                     /locus_tag="ID725_RS18550"
                     /old_locus_tag="pgaptmp_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGV7852214.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            10768..11688
                     /gene="ldtB"
                     /locus_tag="ID725_RS18555"
                     /old_locus_tag="pgaptmp_003711"
     CDS             10768..11688
                     /gene="ldtB"
                     /locus_tag="ID725_RS18555"
                     /old_locus_tag="pgaptmp_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGV7852215.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(11746..12864)
                     /gene="ybiR"
                     /locus_tag="ID725_RS18560"
                     /old_locus_tag="pgaptmp_003712"
     CDS             complement(11746..12864)
                     /gene="ybiR"
                     /locus_tag="ID725_RS18560"
                     /old_locus_tag="pgaptmp_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGV7852216.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(12861..13328)
                     /gene="mntR"
                     /locus_tag="ID725_RS18565"
                     /old_locus_tag="pgaptmp_003713"
     CDS             complement(12861..13328)
                     /gene="mntR"
                     /locus_tag="ID725_RS18565"
                     /old_locus_tag="pgaptmp_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGV7852217.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            13514..13642
                     /gene="mntS"
                     /locus_tag="ID725_RS18570"
                     /old_locus_tag="pgaptmp_003714"
     CDS             13514..13642
                     /gene="mntS"
                     /locus_tag="ID725_RS18570"
                     /old_locus_tag="pgaptmp_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGV7852218.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            13914..15497
                     /gene="opgE"
                     /locus_tag="ID725_RS18575"
                     /old_locus_tag="pgaptmp_003715"
     CDS             13914..15497
                     /gene="opgE"
                     /locus_tag="ID725_RS18575"
                     /old_locus_tag="pgaptmp_003715"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGV7852219.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(15546..16061)
                     /gene="ompX"
                     /locus_tag="ID725_RS18580"
                     /old_locus_tag="pgaptmp_003716"
     CDS             complement(15546..16061)
                     /gene="ompX"
                     /locus_tag="ID725_RS18580"
                     /old_locus_tag="pgaptmp_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGV7852220.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(16114..16179)
                     /locus_tag="ID725_RS18585"
                     /old_locus_tag="pgaptmp_003717"
     CDS             complement(16114..16179)
                     /locus_tag="ID725_RS18585"
                     /old_locus_tag="pgaptmp_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852221.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            16414..>17298
                     /gene="rhtA"
                     /locus_tag="ID725_RS18590"
                     /old_locus_tag="pgaptmp_003718"
     CDS             16414..>17298
                     /gene="rhtA"
                     /locus_tag="ID725_RS18590"
                     /old_locus_tag="pgaptmp_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGV7852222.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         4189 a         4625 c         4385 g         4099 t
ORIGIN      
        1 aaagatggat atggcatgta aaggcaggac ccagcgctac gcccgaggtg gcatacagtc
       61 tgtggcgttt ttctgacata ataggcaaat tcgattttgc ctccgcagga gtgttttcat
      121 ggaatttacc accggattga tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt
      181 caccccactg accgcccagg aaacgctgcc actggttcag tgttttggtc gtattctggc
      241 gagcgatgtc gtttcgccgc tggatgtccc agggtttgat aactccgcaa tggatggtta
      301 cgcggtacgt ttggccgata tcacctccgg acaaccgctg cccgttgccg gtaaatcctt
      361 tgccggtcag ccgtaccatg gtgaatggcc tgcgggtacc tgcattcgta ttatgaccgg
      421 tgcgccggtg ccggaaggct gcgaagcggt ggtgatgcag gagcagactg aacaaacgga
      481 caatggcgtg cgttttactg ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga
      541 agatatctct gcaggtgcgg ttgttttccc ggcggggact cgcctgacta ccgcagagct
      601 gccagtaatt gcttcactgg ggattgccga agttccggtg attcgtaaag tgcgtgtagc
      661 gcttttttct accggtgatg aactccagtt gccaggccag ccgctgggcg acggccaaat
      721 ctacgatacc aaccgtctcg ccgtacacct gatgttagaa cagctgggtt gcgaggtaat
      781 caacttaggg attatccgcg acgatccaca tgccctgcgc gccgcattta ttgaagccga
      841 cagccaggcg gatgtggtga tcagttccgg cggtgtttca gtgggtgaag cggattacac
      901 caaaacgatt cttgaagagc tgggggagat cgccttctgg aagctggcga ttaaaccagg
      961 taaaccgttc gcgttcggta aactcagcaa tagctggttc tgcggcctgc cgggcaaccc
     1021 ggtttcagcg acgctgacct tctatcaact ggtacagcct ttgctggcaa aactaagcgg
     1081 caacaccgcc agcggcctgc ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa
     1141 aacgccagga cgtcttgatt tccagcgcgg cgtgctgcac cgcaacgccg atggcgaact
     1201 ggaagtgacg accaccggac atcagggttc acatatattc agctccttta gcctcggcaa
     1261 ctgctttatc gtgctggaac gcgatcgcgg caatgtggaa gtgggcgaat gggtggaagt
     1321 agaaccgttt aacgcgttgt tcggaggcct gtaatggcgg aactcagcga tcaagagatg
     1381 ctgcgctaca accggcaaat cattctgcgc ggttttgatt ttgacggtca ggaggcgctg
     1441 aaagattctc gtgtgctggt cgtcggcctc ggtggactcg gctgcgcagc ttcgcagtat
     1501 ctggcaagcg ccggtgtcgg caacctgacg ctgctcgact tcgactcggt ttcgctctcg
     1561 aatctgcaac gccagacgct gcacagtgat gctacggtcg ggcaaccgaa ggtggaatcc
     1621 gcccgtgacg ctctgacgcg gatcaaccca catatcgcga ttacgccagt caatgcactg
     1681 ctggatgacg cagaacttgc agcattgatt gctgaacaca atcttgtgct cgactgtacg
     1741 gataacgttg cggtacgtaa tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg
     1801 gtttccggcg cggcaattcg tatggaaggg caaatcacgg tcttcactta tcaggacggc
     1861 gaaccgtgct atcgctgcct tagccgtttg tttagcgaga acgtgttaac ctgcgtagaa
     1921 gcaggcgtga tggcaccgtt gatcggcgtg attggttcgt tgcaggcaat ggaagcgatc
     1981 aaactgctgg caggttatgg caaaccggcc agcgggaaaa tcgtcatgta cgatgcgatg
     2041 acctgtcagt ttcgcgaaat gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag
     2101 taattgccac ctccaggcct gataagacgc gacaagcgtc gcatcaggca tttgctccag
     2161 acgccagatg cggcgtgaac gccttatctg gcctacggtt cgggcacaga tttccaagcg
     2221 cggcaggcat cggtgcactc cgccacgtca tcagcaattt atgaggatgc agaacgtccg
     2281 gttaaatcga cgttctgcca aacgctccct gccagtcctg ctcaaacttc gccacagcgg
     2341 catcaaccgc cggatagcta atcatctgtt gtgccacatc cagtggcaga gtaattgatt
     2401 cacatcctgc cagtaagcag tccagcgcct gacgcggggt tttgaaactc gcggctaaca
     2461 ctttcgcccg cggcgcatgc attttcaata actggtgtaa gtcggtcaca gtctgaatac
     2521 cgctaccgcc ctgagcatca atacgattaa cgtaaggcgc aacatattcc gcacctgcca
     2581 gcgccgacag cagcccttgt gctgcgccat ataccgcggt tcccagcgtc ggaatccctt
     2641 ccgcttttaa catcttaata gctgccagcc cctcggtggt caccggaact ttcaccacga
     2701 tatccgcaat aatagaacgc aatttacgtg cgtcattcac catcccttca gcagtggtag
     2761 ccattacctg ggcaaacaga cgcccctgac cgcccatcgc ttcatgaagt tccggaagca
     2821 caacctccag cggtttttta cccgcggcga taatgcttgg gttagtggtc acacccgcca
     2881 gcggaaaaat acgtgacagc gccttcaccg caacaacgtc tgaagtatcc agatacagtt
     2941 ccataaccat cctcaaaatg tactttttgt ctgaatgtag actatcacgg gaaaactctc
     3001 tgaacagttg acgcacatca atctaacttt cattcgaaag tattttaata tttgaacgaa
     3061 acagagaggc aactatgatt ttcaatattc agcgctactc gacccatgat ggccccggta
     3121 tccgcacggt cgtatttctt aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg
     3181 aaagccgcgc ccgcacgcag gatctgctgt atgacccacg tctgtgcctg gaaggctgtg
     3241 agctgtgcgc taagaccgcg ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc
     3301 atcgggaaaa gttaaccccg gagcatctga cggcgttaac cgactgctgc ccgacacagt
     3361 cgttaaccgt gtgtggggaa gtgaaaagcg ttgaggagat catggtgacc gttctgcgcg
     3421 ataaaccgtt ttacgatcgc agcggcggcg gcttaacact ttctggcggt gaaccattca
     3481 tgcagccgga aatggcgacg gcgctactgc aagccagcca cgaggccggc attcatactg
     3541 cggtagaaac ctgcctgcat gtgccgtgga aatatatcgc cccttctctg ccctatatcg
     3601 atctgtttct tgccgattta aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg
     3661 gtaacgccgc cagggtgctg gataacctga aaaaactcgc cgcagcgggc aaaaaaatca
     3721 ttatccgcgt gccgctgatt caaggcttta atgccgacga aacctctgtg aaagccatta
     3781 ccgattttgc cgccgacgag ctgcacgttg gcgaaattca tttcctgccc taccacacgc
     3841 tgggcatcaa caaatatcac ttacttaatc tgccctatga cgccccggaa aaaccgcttg
     3901 atgcgccaga actgctcgac tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga
     3961 ccttacgagg ataacaacca tgaccacact gaaactggac acgctcagcg accgcattaa
     4021 agcgcacaaa aatgcgctgg tgcatattgt gaaaccgccg gtttgtaccg agcgcgcgca
     4081 gcactatacc gagatgtatc aacaacatct cgataagccg atccctgtac gtcgcgcgct
     4141 ggcgctggca catcacctgg cgaatcgcac tatctggatc aagcacgatg agttgatcat
     4201 cggcaaccag gcaagcgaag ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg
     4261 gatcgaaaaa gagattgatg atctggcaga tcgtcccggt gctggctttg cggtgagcga
     4321 agagaacaaa cgcgttctgc atgaagtgtg cccgtggtgg cgcggtcaga cggtacagga
     4381 tcgctgctac ggcatgttta ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa
     4441 agcggaaggc aatatgacct ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct
     4501 ggaaaaaggc ttgatggtct gcgcgagaaa gtagcggaac gtcgctcgcg catcaatctg
     4561 acggtactgg aagatctgca cggcgaacag ttcctgaaag cgattgatat cgtgctggtg
     4621 gcagtcagtg aacacattga acgtttcgct gccctggcgc gtgcaatggc cgcgaccgaa
     4681 acccgcgaaa gccgtcgcga tgaactgctg gcgatggcag aaaactgcga tcttatcgcc
     4741 caccagccgc cgcagacttt ctggcaggcg ctgcaactgt gttacttcat ccagttgatt
     4801 ttgcagatcg aatctaacgg tcactcagta tcgtttggtc gtatggacca gtatctctac
     4861 ccgtactatc gccgcgacgt tgaactcaac cagacactgg atcgcgaaca cgccatcgaa
     4921 ttgctgcata gctgctggct gaaactgctg gaagtgaaca aaatccgctc aggctcacac
     4981 tcaaaagcct ctgcgggaag tcctctgtat cagaacgtca ccattggcgg acaaaatctg
     5041 gttgatggtc aaccaatgga cgcggtgaat ccactctctt acgcgatcct cgaatcctgc
     5101 ggtcgcctgc gttccactca gcctaacctc agcgtgcgtt accacgcggg aatgagcaac
     5161 gatttcctcg acgcctgcgt acaggtgatc cgttgcggct tcgggatgcc ggcgttcaac
     5221 aacgacgaaa tcgtcatccc ggaatttatt aaactcggta ttgaaccgca ggatgcttac
     5281 aactatgcag cgattggttg tatcgaaacc gccgtcggtg gcaaatgggg ctatcgctgt
     5341 accggcatga gctttatcaa cttcgcccgc gtgatgctgg cggcgctgga aggcgggcgt
     5401 gatgccacca gcggcaaagt gttcctgcca caagaaaaag cgttgtcggc aggtaacttc
     5461 aacaacttcg atgaagtgat ggacgcgtgg gatacgcaaa tccgttacta cacgcgtaaa
     5521 tcaatcgaaa tcgaatatgt cgtcgacacc atgctggaag agaacgtgca cgatattctc
     5581 tgctcggcgc tggtggatga ctgtattgag cgagcgaaaa gtatcaagca aggcggcgcg
     5641 aaatatgact gggtttctgg cctgcaggtt ggcattgcca acctcggcaa cagcctggcg
     5701 gccgtgaaga aactggtatt tgaacagggc gcgattagtc agcaacagct ggctgccgca
     5761 ctggcgaatg acttcgacgg cctgactcac gagcagctgc gtcagcggct gattaacggt
     5821 gcgccgaagt acggcaacga cgatgatact gtcgatacgc tgctggctcg cgcttatcag
     5881 acctatatcg acgaactgaa acagtaccat aacccgcgct acggtcgtgg tccggttggc
     5941 ggcaactatt acgcgggtac gtcgtctatc tccgctaacg taccgtttgg cgcgcaaact
     6001 atggcaacgc cagacggacg taaagcccac cccccgctgg cagaaggcgc aagcccggct
     6061 tccggtactg atcatcttgg ccctactgcg gtcattggct cggtgggtaa actgcctacg
     6121 gcagcgattc tcggcggggt gttgctcaac cagaaactga atccagcaac actggagaac
     6181 gaatctgaca agcagaaact gatgatcctg ctgcgcacct tctttgaagt gcataaaggc
     6241 tggcatattc agtacaacat cgtttcccgc gaaacgctgc tggaggcgaa aaaacatccc
     6301 gatcagtatc gcgatctggt agtgcgtgtc gcgggttatt ccgccttctt caccgcgctc
     6361 tctccagacg ctcaggacga tatcatcgcc cgtaccgaac atatgctgta atcccctcag
     6421 cccggcggag acgtcgccgg gctaaatcat tcacatcatc aatttcatcc ttactttcat
     6481 tcgaaatata atttgtgctc tgcgtcacat tgttattaca ctgttacggt ttcattttcg
     6541 agccgggagc acacctatga gcgtaaaagt tatcgtcaca gatatggacg gtacttttct
     6601 taacgacgtc aaaacgtaca accaaccacg ttttatggcg caatatcagg aactgaaaaa
     6661 gcgcggcatt gagttcgttg tcgccagcgg taatcagtat taccagctta tttcattctt
     6721 tcctgagcta aaggatgaga tctcttttgt cgcggaaaac ggcgcactgg tttacgaaca
     6781 tggcaagcaa ctgttccacg gcgaactgac ccgacatgaa tcgcggattg ttattggcga
     6841 gttgctaaaa gataagcaac tcaattttgt cgcctgcggt ctgcaaagtg catatgtcag
     6901 cgaaaatgcc cccgaagcat ttgtcgcact gatggcaaaa cactaccatc gcctgaaacc
     6961 tgtaaaagat tatcaggaga ttgacgacgt actgttcaag ttttcactca acctgccgga
     7021 tgaacaaatc ccgttagtga tcgacaaact gcacatagcg ctcgatggca ttatgaaacc
     7081 cgtcaccagt ggttttggct ttatcgacct gattattccc ggtctacata aagcaaacgg
     7141 tatttcgcgg ttactgaaac gctgggatct gtcaccgcaa aatgtggtag cgattggcga
     7201 cagcggtaac gatgcggaga tgctgaaaat ggcgcgttat tcctttgcga tgggcaatgc
     7261 tgcggaaaac attaaacaaa tcgcccgtta cgctaccgat gataataatc atgaaggcgc
     7321 gctgaatgtg attcaggctg tactggataa cacatcccct tttaacagct gacctctcta
     7381 ccggagccat gcgctccggt tatcccttct ttttacatcc tgtacatcac atcaaatcat
     7441 taaacttgca ttaacttatt tataacttat attgtcacat gcaaatcagc atagtttcga
     7501 atgaaagtta atgcgaggtg gttatggctt ctacttttac cagcgacaca ttgcctgccg
     7561 atcacaaagc agctatccgt cagatgaagc acgcgctgcg ggcgcagctt ggcgacgtcc
     7621 agcagatctt taatcagcta agcgatgaca ttgccacgcg agtggctgaa atcaacgcac
     7681 tcaaagcaca gggcgatgcc gtctggccgg tgctgtctta tgccgatatc aaagcaggtc
     7741 atgttactgc agagcagcgc gaacagatta aacgtcgcgg ttgtgcggtg ataaaaggcc
     7801 atttcccccg cgaacaagcg ctaggctggg atcagtcgat gctggactat ctggaccgca
     7861 accgctttga cgaggtctac aaaggccccg gcgataattt cttcgggacg ctcagcgctt
     7921 cacgtcccga gatttacccc atctactggt cgcaggcgca aatgcaggcc cgccagagtg
     7981 aagaaatggc gaatgcgcag tcgtttctca atcgtctgtg gacatttgaa agtgatggaa
     8041 agcaatggtt taacccggat gtgagcgtca tctaccctga ccgtatccgc cgccgtccgc
     8101 ccggaacgac ctccaaaggt cttggagcgc ataccgactc cggggcgctg gaacgctggc
     8161 tgcttccagc gtatcagcac gttttcgcta acgtctttaa tggcaatctg gcgaaatacg
     8221 atccctggca tgcggcacat cgtacggaag ttgaagagta cacggtggac aacaccacca
     8281 aatgttccgt gtttcggaca ttccagggct ggacagcgct ctctgatatg ctgcctagtc
     8341 aggggttgct gcacgtcgtg cccattcctg aagccatggc gtacgtactg ttacgtccgc
     8401 tgcttgatga tgtgccggag gatgaactgt gcggcgtagc gcccggaaga gtgttgccgg
     8461 tatcagagca atggcatcca ctgttaattg aggcgttaac cagcattcca aaactcgagg
     8521 ccggagactc cgtctggtgg cactgcgacg tcatccattc cgttgccccc gttgaaaatc
     8581 aacagggttg gggcaacgtg atgtacattc ctgcggcacc gatgtgcgag aaaaatcttg
     8641 cctacgcgca caaggtgaag gccgcactgg aaaaaggcgc atcgccgggc gacttcccgc
     8701 gcgaggacta tgaaacaaac tgggaaggac gctttacgct tgccgacctc aacattcacg
     8761 gtaagcgagc gttgggcatg gatgtttgag agttaattga agtgaattga tgtgggatta
     8821 agaaaatcga ttcgacgtta ctcagattgc tgacaagtgc gctttcttaa tgcctgataa
     8881 gcatagcgca tcaggcaatc ttgcgtttgt catcagccgc cgtcaccccg acagtatcgg
     8941 ggtgacggat aataacttac tcgatccctt tactacgcag gtaatcttcg taattaccgc
     9001 taaagtcgat cacgcgttcc ggggtgattt ccagaatgcg ggtcgccagg gagcttacga
     9061 actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc gccatgttca
     9121 gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc agaatgttcg
     9181 gcttctgcat cattaactta ccaaaaagca tccgcccttt ttctccaccg gaaagcactt
     9241 tagcaggctt tttgatgtcg tcctggctga acagcaaacg accgagaata ctgcgcaccg
     9301 cctgctcgtc atcgccttcc tgcttccact ggctcatcca ttcgaacacg gtcagatcat
     9361 tttcaaactc atattcgtgg tcctgagcat agtaaccaat gcgcgcgttt tcagaccatt
     9421 ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcaacagc gttgatttac
     9481 cgacgccgtt ggtacccagt accgccagtt tttcaccgac ttccagcagc aggttgagat
     9541 ttttaaacag cggaccgtta tcaaaccctt tggtcagacc ttccacttcc agcgcgttac
     9601 ggaacagctt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg gaggctttca
     9661 cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg
     9721 aggcgttggc gctaaagcgg ctaacgaaag attgcagctc agcaatctgc gctttcttct
     9781 tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg tactcatcgt
     9841 agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg gtacagacca
     9901 tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc tcgttcagca
     9961 cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga
    10021 gaatatccgg atcagcaaac agcgcctgcg ccagcaacac acgcagcttc cagccaggag
    10081 caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca agcaacaatt
    10141 caccggcacg agcttccgca gagtaaccgt ccatttcgcc gtatttaact tccagatcgg
    10201 ccactttata gccgtcttct tcactcattt ccggcaaagc atagatgcgg tcgcgctcct
    10261 gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca gtgaactctt
    10321 caaaggcaaa ctgatcctga cgcagtttac caatgcgctc gttgggatcg agggaaacgt
    10381 tacccagcgt cggctcaagg tcgccgccga ggatcttcat aaaggtggat ttaccactac
    10441 cgttcgcgcc aatcaggccg taacggttgc cgccgccaaa tttgacggaa atgttttcaa
    10501 acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca ggcgtatcct
    10561 gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat cctgctcctc
    10621 gcttaagatc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca
    10681 ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc aacccaatgg
    10741 cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt attcgcagcg
    10801 gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc tctgccaacc
    10861 gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg taacactcag
    10921 ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat ggaagcgaac
    10981 ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc gcagcagctg
    11041 atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat
    11101 tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg tcagttaggc
    11161 aaagatacgc ctatcaactg gaccaccaaa gttgagcgta agaaagcagg cccgacctgg
    11221 acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct tccggctgtc
    11281 gttccggcag gtccggataa cccgatgggg ctgtatgcac tctacatcgg tcgcctgtac
    11341 gctatccatg gcaccaacgc taacttcggt atcggtctgc gtgtaagtca tggttgtgtg
    11401 cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg tacccgcgta
    11461 cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg ttacattgaa
    11521 gtccataacc cgctgtctac caccgaagcc caatttgaag gtcaggaaat tgtgccaatt
    11581 accctgacga agagcgtgca gacagtgacc ggccagccag atgttgacca ggttgttctt
    11641 gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc ttcaaaacgt
    11701 aaaacaaagg cggactcata atccgccttt tttatttgcc agaccttacc aggccgggat
    11761 cataaccaac aaaacatatc ccaccagcgc cgcccacaac agcatcggta ttgaatagag
    11821 atggaaacgc caccagatgc ggcgatcgtt cgccatacgt agcgcaatca aatttgccag
    11881 cgatccgggt aataacccaa agccacctac gtttaccgcc catgccagta ataaagacgg
    11941 cggcacatag ttcagcaaca atatagtact cggcacgtta ctgatcacct gcgataaacc
    12001 gattgccgtt aaccataatc cgggttcaga taaatgactc acgttaccca acacgccttg
    12061 caacgctgga agctgggtca gtaaatggac gtcgataaac atcgccataa acaccagcag
    12121 cagcgtccag tccacactca acaccacgcg acgcgccagc agcgcaaagc ccgccgccac
    12181 aatcaccagt ccccacagct cttgtttgaa ctccagcgcc gtcagaaaga cgatatacag
    12241 ccccagacaa ctccacacca gccgcggttt ccactccggt gtttgcaccc ccgtatggta
    12301 ttggagtgcc tttccaggga aacaacacca gcacaacagc aggagcgtca gcatcattgc
    12361 gccagccagc ggtgccattt gggcaataaa tccggcaaac gaaagaccag aacgtcccca
    12421 gataagaata ttttgcgggt tgccaattgg cgtcagtagc gaaccagcgt tgactgccag
    12481 cgcctcaaaa ataatcagcc gattaaccgg gatctcacac agccttttta gcgtgatagt
    12541 cagcggaaca acaataaaca gcgtgacatc gttggtcaga aaggtagaaa gcagcgccgc
    12601 cgccagcacc ataaacatcg ccagccgacg ctccgtagca aagcggcgca ccattttgcg
    12661 ccccagcaca tcaaaataac cgcttaactc cacacctttg gtcagcagca tcaggccgct
    12721 taaggtgatg atggtgtgcc agtcgatagc agcaggccag gatttcggtg caaagggcac
    12781 gaaaaaactt aatccgatac caacaagaat taataactga aaaaaacgat cgccttgcag
    12841 cgtgcgtaaa aaaggcaggc tcatttggca ccgtgtttct gggtaaacaa acgaaaggca
    12901 tccagcgtct cttcactaac atggtgctcc atgccttccg cgtcgcgacg ggcgatttcc
    12961 ggactgacgc ccaacaccaa caagaaattt tcgactatct gatgtcgctc gcggctttcc
    13021 tgcgccagct tctctccttc tgccgttaaa aacacgccac gccaggggat catttcaatc
    13081 agccccatag ttgccagtcg cttaagcatt ttagccaccg tcggttgcga aactcccaaa
    13141 cgagcagcca tgtctacctg acgagcttcc cccacttccc tgatcaagtc agaaatcagc
    13201 tcaacgtaat catcaataag ctcgcgccga tgcgcctctc tgacctggcg gaacccttca
    13261 acgtgctctt cctcgttcac taactgcgtc actttttttg ctgttggcgt acctgcgcga
    13321 cgactcattg tgcttcctca ttatggtgac gcatacatag cgttctcatt tcgtgcgcac
    13381 attgtaaacc agagttgcga aggtacaaaa aattaacgtt ttagcaatag ctatataata
    13441 tagcctgtgc tatatctgta tgtaatgcaa tcatccctca aggatcgacg ggattagcaa
    13501 gtcaggaggt cttatgaatg agttcaagag gtgtatgcgc gtgtttagtc attctccctt
    13561 taaagtacgg ttaatgctgc tctctatgtt gtgcgatatg gtcaacaaca aaccgcagca
    13621 agataaacct tccgataaat agcggcgtcg cggtacgccg cttcactcct gctttcatgc
    13681 aggcacgacg cgttttggtc tgaaaaaccc cactttttgt cggatttgca atccccttcg
    13741 caaaagattt gttcgtcagt agttgacctg aactgcggct cgctctatct tcttgcagcc
    13801 ctgcgtatat tgcggctcgc ggatgcggac ccctttcccc tcttcacgca ctcttgcagg
    13861 tattgaccct tgacgccagg gtaagcacat ggcgtttgtt acgatagtgg catatgaatt
    13921 taaccctcaa agaatcgctt gttacccgta gccgggtatt tagcccgtgg actgcgttct
    13981 actttttaca gtcgctatta attaacctcg gcttaggtta ccccttcagt ttgctctaca
    14041 ccgctgcgtt tacggctatt ttgcttttgc tatggcgaac attgcctcgc gtacaaaaag
    14101 ttctggtcgg tgtcagttcg ctggtggcgg cttgttattt cccttttgct caggcctacg
    14161 gcgcgcctaa tttcaataca ttgctggcat tgcactccac caatatggaa gagtcgaccg
    14221 aaatcctgac gatttttccg tggtacagct acctggtcgg cttatttatt tttgcgctcg
    14281 gcgtaatagc aatcaggcga aaaaaagaga atgaaaaagc gcgctggaat accttcgaca
    14341 gcctgtgtct ggtattcagt gtggcgacat tttttgttgc tcccgtgcaa aacctggcct
    14401 ggggtggcgt atttaaactg aaagatactg gctatccggt atttcgtttt gctaaggatg
    14461 tcatcgtcaa taataacgag gtgattgaag agcaagaacg gatggcaaaa ctttccggaa
    14521 tgaaagatac ctggacggtc actgccgtta agccgaagta tcagacctat gtggtggtga
    14581 tcggtgaaag cgcgcgtcgc gatgccctcg gtgcctttgg cggtcactgg gacaataccc
    14641 cgtttgccag cagcgttaac ggtttgatat ttgctgacta cattgccgcc agtggctcca
    14701 cgcagaaatc gcttggctta acgctcaatc gcgtagtcga tggcaaacca cagtttcagg
    14761 ataactttgt caccctggca aatcgcgcgg gcttccagac ctggtggttt tccaaccagg
    14821 gccaaatcgg cgaatacgat actgctatcg ccagcatcgc caaacgtgca gatgaagtgt
    14881 acttcctgaa agaaggtaat tttgaagcag ataaaaacac gaaagacgaa gcgttactgg
    14941 atatgaccgc tcaagtgctg gcgcaagagc actcgcaacc gcagctgatt gttctgcatc
    15001 tgatgggctc gcatccgcag gcctgcgacc ggacaaaagg caaatacgaa acctttgtgc
    15061 aatcgaaaga aacgtcgtgc tatctctata ctatgacgca aacggacgat ttactgcgca
    15121 agctgtacga tcagttacgc aacagcggca gcagcttctc gctggtttac ttttctgacc
    15181 acggtctggc ctttaaagag cgcggcaaag acgtgcaata ccttgcccat gatgataagt
    15241 atcagcaaaa tttccaggtg ccttttatgg tcatttccag cgacgataaa gcgcatcggg
    15301 tgattaaagc ccgccgctca gccaatgact tcttaggctt tttctcgcag tggacgggaa
    15361 ttaaagcgaa ggaaattaac atcaaatacc cgtttatatc tgagaagaaa gccgggccga
    15421 tatacatcac caacttccag ttacagaagg tggattacaa ccatctcgga accgatattt
    15481 tcgacccgaa gccttaaaaa caaaaatccg ccccgagagg cggatttttt atatcaccaa
    15541 agtgattaga agcggtaacc aacaccggca atccaggtgc ctacgtcaac gctacgaata
    15601 cggctctgct cgtaagagaa gtccagagca acgttttcca tcgggttgaa ctgcagacca
    15661 gcaccgtagg agaaaccgta gtcgctggtg tcgtgtttgt aggtcgggta ttcagtggtc
    15721 tggaatttac cataacccac acccactaca ccgtagatgc ttgcccagtc gttaatgcgg
    15781 taagccggac cagcagtgat gccgtagtac tggtttttgt tgtagtcacc agagcttgca
    15841 gtacggcttt tctcggtgta agtgaatgaa ccgatcacac ccagcgggct gttgtcttct
    15901 tcatagcggt atttcaggtt gaaaccgccc attttgttca tttggccctg agcgtcgctc
    15961 tgtgcgtaac cgccagttac ggtagaagtc gcagctacgg aagtacctgc ggtgaaagcc
    16021 agaactgcgg ccagtgctga aagacatgca atttttttca taaccacctc aaatgtgatt
    16081 caaataagtc ctaagtttta aatatatcaa aaattaatgg gaaactcttc gcgatttgtg
    16141 atgtctaacg ggccatttca tgtaacagaa cgtttccata caccgctatc catctaaatt
    16201 taaatcactt tttcagagaa ctgcgtaagt attacgcatg ttttccctgt cattcatcca
    16261 gattattcct aatcaccaga ctaatgattc catcaatcct ggcgcatttt agtcaaaacg
    16321 ggggaaaatt ttttcaacaa atgctcaacc agcattgggt atatccagta cactccacgc
    16381 tttacttaag tctagatatt tgtgggagaa aggatgcctg gttcattacg taaaatgccg
    16441 gtctggttac caatagtcat attgctcgtt gccatggcgt ctattcaagg tggagcttcg
    16501 ttagctaagt cactttttcc tctggtgggc gcaccgggtg tcactgcgct gcgcctggca
    16561 ttaggaacgc tgatcctcat cgcgttcttt aagccatggc gactgcgctt tgccaaagag
    16621 caacggttac cgctgttgtt ttacggcgtt tcgctgggtg ggatgaatta tcttttttat
    16681 ctctctattc agacggtacc gctgggtatt gcggtggcgc tggagttcac cggaccactg
    16741 gcggtggcgc tgttctcttc tcgtcgcccg gtagatttcg tctgggttgt gctggcggtt
    16801 cttggtctgt ggttcctgct accgctgggg caagacgttt cccatgtcga tttaaccggc
    16861 tgtgcgctgg cactgggggc cggggcttgt tgggctattt acattttaag tgggcaacgc
    16921 gcaggagcgg aacatggccc tgcgacggtg gcaattggtt cgttgattgc agcgttaatt
    16981 ttcgtgccaa ttggagcgct tcaggctggt gaagcactct ggcactggtc ggttattcca
    17041 ttgggtctgg cggtcgctat tctctcgacc gctctgcctt attcgctgga aatgattgcc
    17101 ctcacccgtt tgccaacacg gacatttggt acgctgatga gcatggaacc ggcgctggct
    17161 gccgtttccg ggatgatttt cctcggagaa acactgacac ccatacagct gctggcgctc
    17221 ggcgcaatta tcgccgcttc aatgggttct acgctgacag tacgcaaaga gagcaaaata
    17281 aaagaattag acattaat
//
LOCUS       DABSYT020000094        17080 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.094, whole genome shotgun sequence.
ACCESSION   DABSYT020000094 DABSYT020000000
VERSION     DABSYT020000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.094"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..832
                     /gene="espX2"
                     /locus_tag="ID725_RS18595"
                     /old_locus_tag="pgaptmp_003719"
     CDS             <1..832
                     /gene="espX2"
                     /locus_tag="ID725_RS18595"
                     /old_locus_tag="pgaptmp_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HGV7852223.1"
                     /translation="FNEQQGLLDKDNKCYILLSSDNSGRVMRLSHQALISMLEPEVKK
                     KTIWNNYSIYPSLQDTHEVVRDDPETICMRAFPLFAKGWEYAQKNKKHQLILNALGFK
                     GYIRDIFMSAIMRKTDFVLECNNQPTELNSSFSSLMNDSDQWQQHTLKDKHYANLLTM
                     LDLNDASESDKSKIFFCLSAVFANISHSNVFNGIPDASKTLKRYAFALLAKAHSLDES
                     MISNQTFNTYKTVLLDFNNLSNEEANQLRISSLYRDMVRYAQYRFSKVLSEWTPDAWL
                     "
     gene            1116..3266
                     /gene="dinG"
                     /locus_tag="ID725_RS18600"
                     /old_locus_tag="pgaptmp_003720"
     CDS             1116..3266
                     /gene="dinG"
                     /locus_tag="ID725_RS18600"
                     /old_locus_tag="pgaptmp_003720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGV7852224.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3294..4256
                     /gene="ybiB"
                     /locus_tag="ID725_RS18605"
                     /old_locus_tag="pgaptmp_003721"
     CDS             3294..4256
                     /gene="ybiB"
                     /locus_tag="ID725_RS18605"
                     /old_locus_tag="pgaptmp_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGV7852225.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4397..5482
                     /gene="hcxB"
                     /locus_tag="ID725_RS18610"
                     /old_locus_tag="pgaptmp_003722"
     CDS             4397..5482
                     /gene="hcxB"
                     /locus_tag="ID725_RS18610"
                     /old_locus_tag="pgaptmp_003722"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGV7852226.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5549..5608
                     /gene="ybiE"
                     /locus_tag="ID725_RS18615"
                     /old_locus_tag="pgaptmp_003723"
     CDS             5549..5608
                     /gene="ybiE"
                     /locus_tag="ID725_RS18615"
                     /old_locus_tag="pgaptmp_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGV7852227.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5711..5971)
                     /gene="ybiJ"
                     /locus_tag="ID725_RS18620"
                     /old_locus_tag="pgaptmp_003724"
     CDS             complement(5711..5971)
                     /gene="ybiJ"
                     /locus_tag="ID725_RS18620"
                     /old_locus_tag="pgaptmp_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGV7852228.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6236..6502)
                     /gene="ybiI"
                     /locus_tag="ID725_RS18625"
                     /old_locus_tag="pgaptmp_003725"
     CDS             complement(6236..6502)
                     /gene="ybiI"
                     /locus_tag="ID725_RS18625"
                     /old_locus_tag="pgaptmp_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGV7852229.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLC"
     gene            complement(6576..7262)
                     /gene="ybiX"
                     /locus_tag="ID725_RS18630"
                     /old_locus_tag="pgaptmp_003726"
     CDS             complement(6576..7262)
                     /gene="ybiX"
                     /locus_tag="ID725_RS18630"
                     /old_locus_tag="pgaptmp_003726"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGV7852230.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(7304..9586)
                     /gene="fiu"
                     /locus_tag="ID725_RS18635"
                     /old_locus_tag="pgaptmp_003727"
     CDS             complement(7304..9586)
                     /gene="fiu"
                     /locus_tag="ID725_RS18635"
                     /old_locus_tag="pgaptmp_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGV7852231.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9851..10111)
                     /gene="mcbA"
                     /locus_tag="ID725_RS18640"
                     /old_locus_tag="pgaptmp_003728"
     CDS             complement(9851..10111)
                     /gene="mcbA"
                     /locus_tag="ID725_RS18640"
                     /old_locus_tag="pgaptmp_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGV7852232.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10387..11313
                     /gene="rlmF"
                     /locus_tag="ID725_RS18645"
                     /old_locus_tag="pgaptmp_003729"
     CDS             10387..11313
                     /gene="rlmF"
                     /locus_tag="ID725_RS18645"
                     /old_locus_tag="pgaptmp_003729"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGV7852233.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(11310..13535)
                     /gene="ybiO"
                     /locus_tag="ID725_RS18650"
                     /old_locus_tag="pgaptmp_003730"
     CDS             complement(11310..13535)
                     /gene="ybiO"
                     /locus_tag="ID725_RS18650"
                     /old_locus_tag="pgaptmp_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGV7852234.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13652..14374)
                     /gene="glnQ"
                     /locus_tag="ID725_RS18655"
                     /old_locus_tag="pgaptmp_003731"
     CDS             complement(13652..14374)
                     /gene="glnQ"
                     /locus_tag="ID725_RS18655"
                     /old_locus_tag="pgaptmp_003731"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGV7852235.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14371..15030)
                     /gene="glnP"
                     /locus_tag="ID725_RS18660"
                     /old_locus_tag="pgaptmp_003732"
     CDS             complement(14371..15030)
                     /gene="glnP"
                     /locus_tag="ID725_RS18660"
                     /old_locus_tag="pgaptmp_003732"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGV7852236.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15168..15914)
                     /gene="glnH"
                     /locus_tag="ID725_RS18665"
                     /old_locus_tag="pgaptmp_003733"
     CDS             complement(15168..15914)
                     /gene="glnH"
                     /locus_tag="ID725_RS18665"
                     /old_locus_tag="pgaptmp_003733"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGV7852237.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16318..16821)
                     /gene="dps"
                     /locus_tag="ID725_RS18670"
                     /old_locus_tag="pgaptmp_003734"
                     /gene_synonym="pexB"
     CDS             complement(16318..16821)
                     /gene="dps"
                     /locus_tag="ID725_RS18670"
                     /old_locus_tag="pgaptmp_003734"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGV7852238.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         4197 a         4216 c         4496 g         4171 t
ORIGIN      
        1 tttcaatgag cagcaaggat tgttagataa agataataaa tgttatatcc tgttatcatc
       61 cgataactct gggcgagtaa tgcgcttatc gcaccaggcg ttaatctcaa tgcttgagcc
      121 agaggttaag aaaaagacta tctggaataa ttactctatt tatccatctt tgcaggatac
      181 ccatgaagtt gtcagagatg atccagaaac gatttgtatg cgtgcatttc ctttatttgc
      241 aaaaggatgg gaatatgcgc agaaaaataa gaaacatcaa ttgatcctca atgctcttgg
      301 attcaaaggt tatattcgtg atatatttat gtcagcaata atgcgaaaaa cagattttgt
      361 gctcgaatgc aataatcaac cgacagaact taattcatca ttttcctctt tgatgaacga
      421 ttcagatcaa tggcaacagc atactctgaa agataaacat tacgctaatc tattaacgat
      481 gctggatctc aatgatgcca gtgaaagtga taagtctaaa atatttttct gcctgtctgc
      541 cgtatttgca aatatttctc acagtaatgt ctttaacggt attcctgatg cgtcaaaaac
      601 tttaaaacgt tatgcatttg ctttattggc gaaagctcat tctttagatg aaagtatgat
      661 aagcaatcaa acttttaata catacaaaac ggtattgctt gatttcaata atctgtctaa
      721 tgaagaggcc aaccagttgc gcataagttc gttatatcgt gatatggtcc gctatgcaca
      781 atatcgtttt tctaaagttt tatccgagtg gacgccagac gcatggctgt aaagatcgca
      841 gcagaataat ggcattgatg gccctggctc aggccagggt cggcaatacc ggttcctgcg
      901 aagcgatgac ggcggcaatt gtaaccacga aatgcggtat cacttgctgt tttctgccct
      961 gaagactggc gctcactgtg ccgtcgaatt ttcaatcaaa tatattgcga gagggcgtgt
     1021 ttgcgagccg ctttccagaa acagaaaaac cattacccct gaaaaccgaa aaatgccaca
     1081 atattggctg tttatacagt atttcaggtt ttctcatggc attaaccgcc gcgcttaaag
     1141 cgcaaattgc cgcctggtat aaggcgcttc aggaacagat ccccgacttt attccccgtg
     1201 cgccgcagcg gcagatgatt gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc
     1261 atctggcgat tgaagccccc actggcgttg ggaaaacgct ctcctatttg attcccggca
     1321 tcgccattgc ccgcgaagag caaaaaacgc tggtggtgag taccgccaac gtggcattgc
     1381 aggatcagat ctacagcaaa gatttgccac tgctgaaaaa gatcattccc gatcttaaat
     1441 tcactgccgc ttttgggcgt gggcgctacg tttgcccgcg taatctgacg gcgcttgcca
     1501 gcactgagcc cacgcaacag gatctgctgg cgtttcttga cgacgaactg acgccaaata
     1561 atcaggaaga gcaaaaacgt tgtgcgaagc tgaagggcga tctcgacact tataaatggg
     1621 atggtctgcg cgatcatacg gatatcgcta tagatgacga tctctggcgt cgtttaagta
     1681 ccgacaaagc cagctgcctc aaccgcaact gttactacta tcgcgaatgc ccgttttttg
     1741 tcgctcgtcg ggaaattcag gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg
     1801 cggcgatgga aagcgaagcc gtattgcctg acccgaaaaa tttactgctg gtgctggacg
     1861 aaggccatca cctgccagat gtggcgcggg atgcgctgga gatgagtgcc gaaatcaccg
     1921 cgccatggta tcggctacag ctggacttgt tcacgaaact ggtcgctacc tgcatggagc
     1981 agtttcgccc gaagaccatc ccgccgttgg cgatccctga acgtttgaat gcgcattgtg
     2041 aagagctgta tgagcttatc gcctcattaa acaacattct caatctctac atgcctgccg
     2101 ggcaggaagc agaacaccgt tttgcgatgg gcgaactgcc tgatgaagtg ctggagatct
     2161 gccagcggct ggcaaaactc accgagatgc tgcgtggtct ggcggagtta tttcttaacg
     2221 atttaagtga gaaaaccggc agccatgaca ttgtacgtct gcatcggttg attttgcaga
     2281 tgaaccgcgc gttggggatg ttcgaggcgc aaagcaaact ctggcggctg gcttcgctgg
     2341 cgcaatcttc cggtgcaccg gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc
     2401 tacacctctg gtttcactgc gtgggaatac gtgtcagcga tcagctggaa aggctgctgt
     2461 ggcgcagtat tccgcacatt attgtcacct ccgcaacctt gcgttcgctg aacagttttt
     2521 cgcgtttgca ggagatgagc gggctgaaag agaaagcggg cgaccgtttt gtggcgctgg
     2581 attccccctt taaccactgc gaacagggca aaattgttat tccccggatg cgcgttgagc
     2641 cttccatcga caacgaagaa cagcatattg ctgaaatggc ggcctttttc cgtgagcagg
     2701 tggagagcaa aaaacatctc ggtatgttgg tgctgtttgc cagcgggcgt gcgatgcagc
     2761 gctttctcga ctatgtgacg gatttacgtc tgatgttgct ggtgcagggc gatcagccgc
     2821 gttaccgttt agttgaactg caccgcaaac gcgtcgccaa cggtgagcgt agtgtgctgg
     2881 tgggcttaca gtcatttgcc gaagggcttg atttgaaagg tgatatgctc agccaggtgc
     2941 atatccacaa aatcgctttt ccgcctatcg acagcccggt ggtgatcact gaaggggaat
     3001 ggctgaaaag cctcaaccgc tatccatttg aggtgcaaag cctgccgagc gcctcgttta
     3061 acctgattca gcaggttggg cgactgattc gaagtcacgg ttgctggggc gaagtggtga
     3121 tttacgataa acgcttgctg accaaaaatt atggcaagcg actactggat gcattaccgg
     3181 tatttccgat agagcaaccg gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga
     3241 aatccccacg ccgtcggcgg cgttaatgat gtgagtcagg taaggagtcg taaatggact
     3301 atcgcaaaat cattaaagag atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg
     3361 atactgcgcg cggtctgtat gcccatatgc tcaatggtga agtccctgac cttgagttgg
     3421 gcggtgtatt gattgcgctg cgtatcaaag gggaagggga agcagagatg ctcggctttt
     3481 acgaagccat gcaaaatcac accatcaagc tgacgccgcc tgcaggcaag ccgatgccga
     3541 ttgtcatccc cagttacaac ggcgcgcgta aacaggccaa cctgacgccg ttgttggcga
     3601 ttctcctgca caaactcggt tttcctgtgg tggttcacgg ggttagcgaa gatccaactc
     3661 gcgtgctgac cgaaaccatt ttcgaactga tgggtattac gccaacgttg cacggcgggc
     3721 aggcgcaggc gaagctcgac gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc
     3781 cgccgctgga aaaacaactg gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata
     3841 ccctggcgaa actggcgacg ccatttgccg aaggtgaggc actgcgtctt tccagcgttt
     3901 cgcatccgga atacatcggg cgagtcgcga agttctttag cgatatcggc gggcgggcgt
     3961 tactaatgca tggtactgaa ggcgaagtgt atgctaatcc gcagcgctgc ccacagatca
     4021 atctcattga tcgtgaagag atgcgggtgc tgtatgaaaa acaggaccct gctggaagcg
     4081 agttactgcc acaagcaaaa gatccggaaa ccacggcgca gtggattcag cgttgccttg
     4141 ctggccgcga accgattccc gaatcgctga aaatccagat ggcttgctgc ctggtggcta
     4201 cgggtgaagc ggcaactatc agcgatggcc tggcgcgcgt taatcaggca ttttaattct
     4261 tttctcagcc ggatgagcca tgtcatccgg cttttacccc accgcaatat gaaattcctg
     4321 catctttatt gaccttcccg cgcccggcgt gcagcataaa aaaacaacaa acacataaca
     4381 taaacaggag ttaaccatgg aaagtggtca tcgctttgat gctcagacgc tgcacagctt
     4441 tattcaggct gtatttcgtc agatgggtag cgaggaacaa gaagcgaaat tagttgccga
     4501 tcatttaatc gcggcaaacc tggcagggca tgattcacat ggtattggca tgatcccaag
     4561 ctatgtacgc tcctggagtc aggggcacct gcaaattaac catcatgcca aaatcgttaa
     4621 agaggcgggg gcggcggtca cgctcgatgg cgatcgcgca tttggtcagg tcgcggcaca
     4681 tgaagcgatg gcgctgggga ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct
     4741 acataactcg catcatatcg gccgtatcgg ttactgggcg gagcagtgtg cagcggcggg
     4801 gtttgtctct atccactttg ttagcgtggt cggtattcca atggtcgcac cgttccacgg
     4861 tcgcgacagc cgctttggca cgaatccgtt ctgtgtggtt ttcccacgta aagatgattt
     4921 tccgctgttg cttgattacg ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg
     4981 gcataaaggc gtccccgtgc cgccaggttg cctgattgac gttaacggcg tgccgacgac
     5041 caatccggcg gtaatgcagg agtcgccgtt gggttcgctg ttgacctttg ccgaacataa
     5101 aggctacgcc ctggcggcaa tgtgtgaaat tcttggcggg gcgctttctg gcggtaaaac
     5161 gacgcatcag gaaacgttac aaaccagtcc cgatgccatt cttaactgca tgaccactat
     5221 catcatcaac ccggaactgt tcggcgcgcc ggattgtagc gcgcagaccg aagcctttgc
     5281 cgagtgggtg aaagcctcgc cgcatgatga cgataagccg attttgctcc cgggcgagtg
     5341 ggaagtgaac acgcgtcgcg aacggcagga gcaggggatt ccattggatg cgggaagctg
     5401 gcaggccatt tgtgatgcgg cgcggcagat tggtatgccg gaagagacgt tgcaggcttt
     5461 ctgtcagcag ttagccagct aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt
     5521 aacataggat caatgagggt tagagcatat gcgtctgtcg gcaaacagac agggaaatac
     5581 ttgtgcagga cgtagcgtaa acgcctgatc cgtccaacgg ttcggatttt gtaggcctga
     5641 taagacgcgg tagcgtcgca tcaggcatct gttgccggat gcggcgtaaa cgccttatcc
     5701 gtcctacgaa ttacttataa attaccgcag taccgcttaa tttattgttg ttggtggcgg
     5761 aagtgatgct atatccactg gcaccggctg ccgccgcttt ctcagccagt ttcgcttcca
     5821 gggcatcgag ggtggatgcg ccatcggcag aaaccacgcc gattttattc atgttctgtg
     5881 cctgggatgc cgttaccggt tccgcggcaa acacgccaaa tgacagagct gaaagagcca
     5941 tagcagcaac aacagtattg atagttttca taattaatct ctcgcaggtg atttttgtat
     6001 aagtgacgtt gtttcgtcga tgtgatgagt atcacgtttt tttacgagag ataaaatcga
     6061 agagaattga ccgccttgtt caaataaatt gattgatatc taacatactg ataaaccatt
     6121 tatttgttgt acttatggac ttgttttatg cagcgtggca gtcacgacag cgttaaaggc
     6181 acggaatgcc acgcaaaata caaattatac tcgctataca tctcgccagt tgtagttaac
     6241 ataactggct ttctttcgaa cctctgcgat tatatcctgt ataagctggt ttttgtaaat
     6301 ctttctcctg ctgacaatga atacataagc gcacgccagg aatggcttcc cgacgggctt
     6361 gcgggatggg ggcaccgcac tcttcacatt catacaggct ttcgccacgc ggaatttcac
     6421 cccgagcgcg ggcaatcgca tcttcaattg tactgttgat ctgttcgttg acggcgtcat
     6481 cgttagccca accggatgcc atatcgtcct ccccatatca atgcttgtat agttaagtgt
     6541 agctaatata gggacgaacc tgggcagttc aagcgtcaga tctccgacca ttcccgcagc
     6601 agattatgat aaagattgag cagcgacagg atctcttcat tttcaccata gcggcttttc
     6661 agcgactgaa tattctgaat atttttgtcc agttcaaaca gcatggcgcg ctttttatca
     6721 tcgcggatca tcgactggat ccatataaat gatgccactc gtacgccgcg ggttacgggt
     6781 gtcacgcaat gcaggctgct ggagggatac aacaccagat cacctgccgg gagttttacc
     6841 cgatgttgtc cgaaggtgtc attaacgacc agttcgccgc cgtcgtagct ttgtggatcg
     6901 cttaaaaaca gcgtggcaga aaggtcagtg cgcatccagc cgttttgtgg atggctgcgt
     6961 actgcgccat ccacatgaaa accataggtt tcattgttct gatagcgatt aaacagcggc
     7021 gtggaaaggg tacgcggcaa ggccgcggca aagaacaaag catgttggtt aaccgcgttc
     7081 agcacctcat tttgcagggc ggcgtataac gtgctgcggg tgtctacctg ttgattgttc
     7141 ttaacttgcg caccttgtgc gccggtggtg acgcgtccat ccacccattc ggcttgttcc
     7201 agttgttcgc gaaaacgggc gacgtcctgt ggcgataaca cgccgggaat gtggtacatc
     7261 atacaagttc tccaaaaagt ggggcctgcg ccccacatct gaatcagaaa tgcatattgg
     7321 ctgtgagcaa gaaggttctt ggctcgcccg ggtgataacg gtagccgctc ttattgattg
     7381 aggcgacgta atcggtatca aacaggttgt agacgtttag ctggaagtcg agattgcgat
     7441 taaccctata ccccagtttg gcatcggcga cccagtaacc ttcggtaaac gctggcgttc
     7501 ccaccgcgcc gtctgaacct ttatgcatac tgccgatata gcgtgcgccc gcgccaacag
     7561 agatatcatc ggttgcctga tattggctcc ataaggtgaa ggcgtgctcc ggggtatacg
     7621 gcagcgatga ggaaccatcc tgggcaacat ctttgccgtt tttgatggtt gctttttgct
     7681 gggtatagcc gccaatcacc tgccacgcgg gagtgatatt ccctgccacg gatatctcat
     7741 agccttcgac gcgtttctta ccgtattgcg agtaagtccc gtcatcattt tgctcaactt
     7801 cattttcgat atcagtacgg aacagcgcgg cggtgagcaa caggcgttta tccagaacct
     7861 gccatttggt gccaatctcg ctggtgttgg ctttttgcgg tttaaaatcg gtgcggttgg
     7921 cactgttacc gctgccagac tgcgcaaggg cgaagttgtt gccgcccgga ggctgctggg
     7981 aaacggcata gttaatatag acattgccgt tttccgtcag gtgatacagc gccccggctt
     8041 tccagttcac cagattgccc gacttggcgg tgtcgacggt ggtgaccgga gaaccttttg
     8101 ccacaccagc tgggcaggtg atggcaccgc gtccgctgcc gccgcaggcg gtggcactgt
     8161 catattcagt atgataatta tccagacgga tcccgccgtt cagctcaaaa tcacgggtga
     8221 tttgcagcgt atcgaatgcg taaattgcga aggtatccgt ctgaccattg gcgtttgcgc
     8281 cgttgcgcgt caggccgccg ggatgaatgc tgctgtcagg atgataaata tttaccgcag
     8341 gtaacgtcac cggattaacg ccgtagttag tctgcgtttc acgggtaaat tccacgccgg
     8401 tactgacatc atgaccgata gaagcggtat agaacgtcga ggtcaggttg gtctggttgg
     8461 tgagaatttt attactcaca tctttggtat tcgccgtgcg tgaccacgtc cagctattca
     8521 catcgctggt gggctgggta atattcgacg ccccacccat aatcgccgtc atcaggtaat
     8581 cctgcttcac gcgcgaccaa cgggtagtgt tgcgaatggt ggtgttatcg ttgatatcgt
     8641 gctcaaaacg catggtggcg gtgtcggtgg tcgaatcgtc gtaatcggaa tccgtgccgt
     8701 aaaagttatg cgtatcaact tttccggaat gattcagggt cgccgttcct gcagatgggg
     8761 cagaatagcc cggcaaaccg atggttggaa tgccgccgtc tggcgtgttg tgctgggtga
     8821 catgcagata attaagatac aaacgattcg ctgtaccaag gccaaaagcg atagaagggg
     8881 cgacgccgta acgctcattt ttgactttgt cgcgtccggc atcgtgcgtt ttttcgccca
     8941 tcacattcag gcgcaccgca gtggtatcac caatgacctg attgacgtcc agcgtgccgc
     9001 ggcggaacca ggcgctgcca atactggcgg aggcgtcaat gccggaatca ttgcgcggct
     9061 gcttgctgat catattgatc gagcccgtcg gcgcgctgcg cccgtagtcg gtgccggaag
     9121 gccctttaat cacttcgacc tgctcagtat tgaaggtgtc gcgcgagacg ctgccgatat
     9181 cgcgaatgcc atcaatataa atactgttag aggtatcagc accgcgcata taaatggcgt
     9241 cgccagtggt ggagttaccg ttctcacccg caaaaaacgc acccacgccg gggacgtttt
     9301 tgagcgcatc ggtaaggttg gttgcgccct gatctttaat cacttgttca gaaatcaccg
     9361 tcatcgtgcg ggtagtatcc gctaccggac gcgagaattt cggatcggca gattgttgtg
     9421 gcgcataaag cgaaggcgtt gatgcttcga caaccagcgt gtcatccgcg ttagtttgcc
     9481 cttcggcggc gagtgcctga gccacaggcg tgatgccaat acaaagaccg gcaaagaacg
     9541 tgagcgaatg aaattgtctg gcagggaaat tgcgattgtt ttccattttg caggtgactt
     9601 tttcttatat gttgaaattt tcactgccac gagaaagcct ctctgcggat gcgatgccac
     9661 tgttaatctc gtgatagtgt tcgtgcaagt gataataatt ttgataagca ttatcacttg
     9721 gcgcgatttt ctatcaccca ttgcgcgaaa aagaaataca tttatttaca ttgcgttcat
     9781 atttgatgtt ttttgattac aaaacaggcg aaaactgcat ccagaggcga atgcagttac
     9841 gttggtgggg ttatttgtag atggttgcag taccgaacat ctggtcattt ccgcctgcgg
     9901 aattaatgac ataacctttc gcgccttgct cgcgcgcttt ttctgccagt ttatcctcca
     9961 gatcgcttag gtttgatgcg ccagttgccg aaacggtacc ggcgggccgt agctgaccgc
    10021 tgtccaggtt actcatcggt tgcgcggcat aagccgtgag agagattccg ctcaggacgg
    10081 tggcaatcag tagtgtgagg cactttttca taatgacatc ctcataaata caacgggtag
    10141 tgcctgataa gtgtaggtcg cctgcgcgat gcaaatgacg caaaaaattg acagagatgt
    10201 gcttattttt tgaacgatat atttttacaa aataagacaa atcaatgaca tgcataatgc
    10261 tgatttttat gatgaattgc cataaaccat agccatgtta aggttttggg gaatgataaa
    10321 cgcagccaga tatttaccct gtcgcgttat gcgggtatct tacgccgctg cttaaaggag
    10381 aatgctatgt ccgcccagaa accggggttg catccgcgca accgtcatca cagccgctac
    10441 gatctcgcca cgctttgtca ggtcaatcct gaactcaagc aattcctcac gcttacaccc
    10501 gccggggagc aaagcgtaga ctttgccaat ccgctggcgg tgaaggcgct caataaggcg
    10561 ttgctggccc atttttacgc cgtagcgaac tgggatatcc cggacggttt tctctgcccg
    10621 ccagtaccgg gccgggcgga ttatattcat caccttgccg atttactggc tgaagcaagc
    10681 ggaacaattc cagctaatgc cagcattctt gatatcggcg ttggtgcgaa ctgtatttat
    10741 ccgctgattg gcgtacatga atatggctgg cgttttaccg gtagtgaaac cagcagccag
    10801 gcgttaagca gcgcgcaggc gatcatcagt gctaatccgg ggcttaatcg cgccattcgt
    10861 ctgcgtcggc aaaaagagag tggggcgatt tttaacggca tcatccataa aaacgagcaa
    10921 tacgacgcga ccttgtgtaa cccgccattc cacgattccg ccgctgcggc gcgggcaggt
    10981 agtgagtgta aacgccgtaa cctggggctg aacaaagacg atgcactgaa ctttggcggc
    11041 cagcaacagg agttgtggtg tgaaggcggt gaggtcacct ttatcaaaaa gatgattgaa
    11101 gagagcaaag gcttcgcgaa gcaggtgatg tggtttactt cactggtatc tcgtggtgaa
    11161 aacttaccgc cgttgtatcg tgccctgacg gacgtgggcg cggtgaaggt ggttaaaaaa
    11221 gagatggccc aggggcaaaa gcagagtcgc tttattgcct ggacctttat gaacgacgag
    11281 cagcgccgcc gttttgtcaa tcgccagcgt taaagcgttg gctcccccgg cggtaacggt
    11341 tcagccgggg tcgcgcccgg agcaggcagc acctgataag tctgcaccgg cgcgcgaacg
    11401 cccgccaggt cgaaatgttt tttcacctgg ctgtcgaggg caaagcgtac cgtccactgt
    11461 ttgagtggca gcgtggtgaa cgaaacacgc agtgtaaacg cggtattgct taagccaaca
    11521 atcccggcaa agttcggttc accaataatc agcccacgaa tttcttcgtt ttccattaat
    11581 tccgctaccg catctttcag tgcctgattg gctttatcag catcttcatg gcgatcaaca
    11641 tcgtagtttg ccactaccga accaatgccg cggacaaagt tggcaaaggt ggtaatcgaa
    11701 gaccacggaa tgatgtggta cgccccggta tcctggcgta cgcccacgga gcgaatcgac
    11761 atccgttcca cagtgccggt caacggccca atagtcacca aatctccagt gttcatgccg
    11821 ttttcaaact gaataaatac cccggtgata atatctttca ccagcgtttg cgaaccaaac
    11881 gagatagcca gacctaatgc cccggcacct gccagcaatg gcgcgatatt gacgccgatt
    11941 tccgacaaca caatcatgat ggtgatggta ctgataatca ccgccagcgc gttacgaaac
    12001 agcgtaagca gggtacgcgt gcgggcgctg ggtagcgggc ggccatgaat atccgaagcc
    12061 agccggtttt cgatcaaact ggcgagcacc gtccagccaa ccgccgagaa gaaaagaatg
    12121 agtgcgatac ggatcaggat atctacggtt ttctgccccg cgccgttttg cagccagttc
    12181 cagaaatcga acaatcccca tgcgctcaac agcagcatta ccgccacgca gacggtcaga
    12241 atacgcgccg ttttcagcgc cgccgaaagc cagccattca gccgtttttg caactccgga
    12301 tagttacgct gagtatgtgg cgagagagtg atggtttttg ccagccagcg ggaaaacata
    12361 ccggaaacaa atgctgcgat accaataatc gccaggctgc gcactgttgc acccatcata
    12421 aatttcaggc tgttgcccgg atcgaacaac gaaaagaaaa acagcacgat aaaataggcg
    12481 cttgccagcc agtgccacac cagcgcaaag gcgcggataa acaggctgaa aaaggccagc
    12541 gaatgctccg caaagttgag caaatgctgg gtaatctctt ttttattacg aaagatcagg
    12601 tacaacgccc agacggtcat gcacagcata atgatgacgt tcgccagcgc acctatctgt
    12661 acattcacct gattagagat aatcggcacg gccacaatca ggccataacc tatcaaactg
    12721 cctaaccagc tcaggcgacg actccagtaa cgggcgctct cgtcctgaat cgtgaacggg
    12781 cgcagttccg ccacgtttgg gcaaaaaatc aggcgtagta cggctttgaa aaattcaatc
    12841 agggcaaagg cgttgagaaa caaactttgt tggaaagcga tggtgcgact gcctgcattc
    12901 aggttgtcgc ttaatatctg gccgacaaat aatgtcagtg ccagtaacag caggtcgata
    12961 ataaacgccc cgataatcat cgccggaagc tgcagccagt tactgcgctc acgatttttt
    13021 tgccgcgccc actgacccat tttgcgatac agcggcagtg cgcacaggcg aatcagccag
    13081 taaaaaccaa acactaaaac cgctaacatt gaaaaatggg tcagcgcatt gctgaaggtt
    13141 tgtggattaa acggcttatg cggggagccg gtgatattgc gataaagctg cccgaagcgg
    13201 gcggaaaggg cttcaccata atggcggctg aattcggtga ccttttgcag cacggtttgc
    13261 tcttcaacca gcgtcggcgg cacgatcttc gggaccggtt cagcaggtgg cgtcgcggcg
    13321 acggtgcgca actggtcgat caactcttta cgcgaggtgt cattatccag cacatccgcc
    13381 agtgcgccat aagccgcttt tttttgttcg atatccggtt ccggggccgg ttcagtcgtt
    13441 gagtccgttg tcgttgtggt tgtaacgccg ggtatggata ccgcgtgggc aggtgcgccc
    13501 agcaggcaga agaggatgaa caggatccac cgcatgactc ctccagtgag aaaacagagc
    13561 aaaaagataa gtatagatgc tgggggaggg gtgattttaa atgagaggaa tctggtgtac
    13621 ctccctttcg ggtgaaaggg aggcagagaa gttaagagac gtgctgtaaa aattcctgca
    13681 agcgctggct cggcggattt ttaatcaaca cttgtggatc gccatcttcc gcaatccggc
    13741 ctttgtcgat aaagatcagg cgcgaagcta ctttctcggc aaaaccgatt tcgtgggtca
    13801 cgatcaccat cgtcatccct tcttcagcca gatcttgcat aaccttcagc acttcatggc
    13861 gcagttccgg gtcaagagcg gaagtcggtt catcaaacag catcattttc ggcttcaccg
    13921 ccagcgcgcg ggcaatcgcc acacgctgct gttgaccacc agaaagttcg gaagggtagt
    13981 gatgtgcacg ttctgccaga ccgactttcg ccagcagctc acgtgccagt ttttccgcct
    14041 cttctttgtt cgcgccacgc acgcgtagcg ggccaaacat gacgttttcc agcgccgtca
    14101 gatgcgggaa gaggtaaaac tgctggaaca ccatacctgc ttcctggcga atcaggcgct
    14161 cgtcaacttt cggatcgtta accttcaggc catcgacaat cagatcgccg gaggtgattt
    14221 cttccagttt gttgatgcag cgcagcaggg tcgatttacc ggaaccggac ggcccgataa
    14281 tcaccacgac ttcgccctgg gcaatgttca aatcgatatt gtgcagcacc tgggttgggc
    14341 caaagtgctt ggagacgttt ttaaattcaa tcacaggatt ttcatccttc tttccagacg
    14401 acgcagaata aagctcagca ccagggtaat aatcagatag aacaccgcca cggcgctcca
    14461 gatctcaagg gcgcggaagt taccggcaat aatttcttgc ccctgacggg tcagttccgc
    14521 cacgccgatc acaataaaca gcgaggtgtc tttaatgctg atgatccact ggttacccag
    14581 cggcggcagc atacgacgca gtgccagcgg taaaatgacg tagcgaatgg tttcccaacg
    14641 tgaaagaccg agcgccagtc ctgcttcacg aaaacctttg tggatagaca gcaccgcacc
    14701 acgcgtgatt tccgcaatgt acgcgccgga gttgatcatg atggtgacca ccgccgcagt
    14761 aaatgggtcg atgcgtaagt cattaaacgc catcggcagg gcaaaataga taaacatcac
    14821 ctggacgacg ataggtgtgc cgcggatcac ttcaataaaa accagcgcga cgtggttggc
    14881 tatccaacca ccgaaggtgc gtgcaaaacc tgccagcaat ccgatcacca ggccgccagc
    14941 cagaccgagg accgatatcc acagggtcat tttggcacct tcaatcagaa gcggaatggc
    15001 aggccagata gcactccagt caaactgcat atgttgttcc tgttaccgtg gtgtaaataa
    15061 tcaaattcag ggtaattaat ggccccggag cgggtttgcg ccctcaccct aatcctctcc
    15121 ccagagggga gagggaactg ccagtgcgct ttacaggtgt agcgttatta tttcggttca
    15181 gtaccgaacc attttttgta gatttcgttg taagttccgt tctcgcgcag ggttttcaac
    15241 gcgccgttga ctttgtcacg cagctcgtcg ctacctttcg ggaacgcaat accgtattgc
    15301 tgcgcttcca gagagtcgcc taccgctttg aactgaccgt taccggcggt tttgatgaag
    15361 tacagaatgt ttggcgtatc gtgcagaacg gcgtctgcgc ggttggtgcc cagttccata
    15421 taggcgttat cgatgttcgg gaactgacgc agatctttag ttttgatgtt tgctttcgcg
    15481 taatcaacgg agccagtgcc gctcttcacc gcaaccactt tcccgtcgag atctttcacg
    15541 cttttcacat cgttattgtt agctttcacc atcactaaca ggccgctttt gtagtagccg
    15601 tcagagaaat cgatcgcttt tttacgctcg tcggtgatgg taatgcccgc cagcgccaga
    15661 tcgacgtttt tggtttgcag tgccggaatg atcccactga aatccatcgg cttcagttcg
    15721 taatccagct tcagctcttt agcgatggca gcccacagat caacgtcaaa gcccacatat
    15781 ttatcgccct gtttaaattc aaacggaacg aaggcggtat ccgtcgcgac aactaatttt
    15841 ttatccgcgg catgagaaga aaccgcaaaa gccagggtca gtgcagccag tgaaactttt
    15901 aatacagact tcatagcatt tccttttttc tatccacggg acgatcccct gcgtgacatt
    15961 cacatatatg aaaaaatcgt gccagttttg caactcattg atttttcgag atgcggcaaa
    16021 aacgtgatgc acgatttata gggcaacacc ctgaagatgc accattctgg ggcaccaaac
    16081 tggtgcgcta aaattgtgca ctcaacacag tgattattta gcgtaaaacc tgatgaaaaa
    16141 acaatcttta tgtaacgatt gtgtgatgat gtggatacaa aaaatttact taatcagctg
    16201 gggatagcag atggatgcac taaataagtg cgttgaggcg ggctgcaaaa caaaacggcc
    16261 tcctgtcagg aagccgcttt tatcgggtac taaaattctg caccatcagc gatggattta
    16321 ttcgatgtta gactcgataa accacaggaa tttatccagg tcgcgagacg cggcggtcag
    16381 gatatctgcg gtgtcgtcat ctttcgcttc gccaatcgct ttgcgtacgt cattagcgac
    16441 gattgcgtaa cggtcagcca gttctttcag gtgatcctga acgttgtgga tgtccagcgg
    16501 gtaacttttc agcggggttt tgctgttgat aacttgagtg gtccccagag ctacaccgcc
    16561 cagctgcact gcacgttctg ccatggtatc cagatgatcg atcagtgcag tgcggaagcc
    16621 atccagcatt tcatgtacgg caatgaagtt agcgccgcgc atgttccagt gcgcttgttt
    16681 ggtaatcaaa gaaagatcaa taaactggat aacctggcga ttcagcaact ctactgttgc
    16741 ttttttctcg ctgtcagaga catcgttgcg ggtataaagc agattggtcg cttttgattt
    16801 aactaattta gcggtactca taatttcata tcctcttgat gttatgtccc agtaattaac
    16861 gagactaagt atagcaccgg ctatgtgttc cgctattctg gctgttccta tcacactaat
    16921 agtggtaaca agcgtgaaaa acaaaactaa taaagattca atgagttaga tatattgata
    16981 agaacaattc ttatttattt gctcgccagg aattttcgga ataaatatgc ttttgcataa
    17041 ttcgatatat aaagacggtg tagaggaaaa gtagcgagaa
//
LOCUS       DABSYT020000095        17042 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.095, whole genome shotgun sequence.
ACCESSION   DABSYT020000095 DABSYT020000000
VERSION     DABSYT020000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.095"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(72..224)
                     /locus_tag="ID725_RS18675"
                     /old_locus_tag="pgaptmp_003735"
     CDS             complement(72..224)
                     /locus_tag="ID725_RS18675"
                     /old_locus_tag="pgaptmp_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGV7852239.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(488..1222)
                     /gene="cysH"
                     /locus_tag="ID725_RS18680"
                     /old_locus_tag="pgaptmp_003736"
     CDS             complement(488..1222)
                     /gene="cysH"
                     /locus_tag="ID725_RS18680"
                     /old_locus_tag="pgaptmp_003736"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGV7852240.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1296..3008)
                     /gene="cysI"
                     /locus_tag="ID725_RS18685"
                     /old_locus_tag="pgaptmp_003737"
     CDS             complement(1296..3008)
                     /gene="cysI"
                     /locus_tag="ID725_RS18685"
                     /old_locus_tag="pgaptmp_003737"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGV7852241.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3008..4807)
                     /gene="cysJ"
                     /locus_tag="ID725_RS18690"
                     /old_locus_tag="pgaptmp_003738"
     CDS             complement(3008..4807)
                     /gene="cysJ"
                     /locus_tag="ID725_RS18690"
                     /old_locus_tag="pgaptmp_003738"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGV7852242.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5126..5488
                     /gene="queD"
                     /locus_tag="ID725_RS18695"
                     /old_locus_tag="pgaptmp_003739"
     CDS             5126..5488
                     /gene="queD"
                     /locus_tag="ID725_RS18695"
                     /old_locus_tag="pgaptmp_003739"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGV7852243.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5566..6837
                     /gene="ygcN"
                     /locus_tag="ID725_RS18700"
                     /old_locus_tag="pgaptmp_003740"
     CDS             5566..6837
                     /gene="ygcN"
                     /locus_tag="ID725_RS18700"
                     /old_locus_tag="pgaptmp_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGV7852244.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            6828..7088
                     /gene="ygcO"
                     /locus_tag="ID725_RS18705"
                     /old_locus_tag="pgaptmp_003741"
     CDS             6828..7088
                     /gene="ygcO"
                     /locus_tag="ID725_RS18705"
                     /old_locus_tag="pgaptmp_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGV7852245.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7105..7680
                     /gene="ygcP"
                     /locus_tag="ID725_RS18710"
                     /old_locus_tag="pgaptmp_003742"
     CDS             7105..7680
                     /gene="ygcP"
                     /locus_tag="ID725_RS18710"
                     /old_locus_tag="pgaptmp_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGV7852246.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7828..8688)
                     /gene="ygcQ"
                     /locus_tag="ID725_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /pseudo
     CDS             complement(7828..8688)
                     /gene="ygcQ"
                     /locus_tag="ID725_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(8685..9464)
                     /gene="ygcR"
                     /locus_tag="ID725_RS18720"
                     /old_locus_tag="pgaptmp_003744"
     CDS             complement(8685..9464)
                     /gene="ygcR"
                     /locus_tag="ID725_RS18720"
                     /old_locus_tag="pgaptmp_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGV7852247.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9442..10778)
                     /gene="ygcS"
                     /locus_tag="ID725_RS18725"
                     /old_locus_tag="pgaptmp_003745"
                     /pseudo
     CDS             complement(9442..10778)
                     /gene="ygcS"
                     /locus_tag="ID725_RS18725"
                     /old_locus_tag="pgaptmp_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10872..12326)
                     /gene="ygcU"
                     /locus_tag="ID725_RS18730"
                     /old_locus_tag="pgaptmp_003746"
     CDS             complement(10872..12326)
                     /gene="ygcU"
                     /locus_tag="ID725_RS18730"
                     /old_locus_tag="pgaptmp_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGV7852248.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12396..13181)
                     /gene="ygcW"
                     /locus_tag="ID725_RS18735"
                     /old_locus_tag="pgaptmp_003747"
     CDS             complement(12396..13181)
                     /gene="ygcW"
                     /locus_tag="ID725_RS18735"
                     /old_locus_tag="pgaptmp_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGV7852249.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13500..14777
                     /gene="yqcE"
                     /locus_tag="ID725_RS18740"
                     /old_locus_tag="pgaptmp_003748"
     CDS             13500..14777
                     /gene="yqcE"
                     /locus_tag="ID725_RS18740"
                     /old_locus_tag="pgaptmp_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGV7852250.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14804..16282
                     /gene="ygcE"
                     /locus_tag="ID725_RS18745"
                     /old_locus_tag="pgaptmp_003749"
     CDS             14804..16282
                     /gene="ygcE"
                     /locus_tag="ID725_RS18745"
                     /old_locus_tag="pgaptmp_003749"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGV7852251.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         4096 a         4445 c         4250 g         4251 t
ORIGIN      
        1 aaatttaaaa aagggtacag gagaccaaac catcacaatt caatacgcgg ggttttaccc
       61 ccgcgtggtg gctacttctt cgattcgtaa gcgagaactg ccttaaactc cataccacgc
      121 tctctgatac tcaactcaca taacgagtcg cctaccatga gcgtgaatcc cagtactgta
      181 caacacagta ctatgattgc cacaagggca tattttgtta gcatgtcttg cctccctgca
      241 aagaagagac taacatccga attgctacgt tcgaacgcga gcctcaggtt gatagaaata
      301 tcgcctgggg cttttgtcca tctggaacct cgcgaatgct taacgccaga cagcctcaag
      361 cacccgacgc cattctatac ctgataattc ttccagcggc gttcacgttt aaaatcagga
      421 atgctgctcg caaaataaaa acgcccccat cattactgac agaggcgttt aatttatccg
      481 gcaatattta cccttcgtgt aacccacatt ccctttttaa gccaaagaaa cgcgtttctt
      541 cttccgccat gccgggttcc catttacggg ttgtatgggt atcgcccacc gataaatatc
      601 cttcgtccca taatgggtga tatttcaggc cattcttttg cagatactga tatatcgttc
      661 ggttatccca gtcgataatc ggcagcactt taaatacgcc acgctgaatt gccagcaccg
      721 gtaaattggc gcgactgccg gattgttcac ggcgcaggcc agcaaaccag gtttgcgcgt
      781 taagttcttt cagcgcccgg ttcatcggtt cgactttgtt gatgtcattg tacttttcaa
      841 tgccttcaac gccctgttcc cacagttttc cgtagcgtgc ttcctgccag gctgcgcttt
      901 cggtagcacg gtacactttc aggttgagct tgagtttgtc cgttaactcg tcaataaagc
      961 ggtaggtttc cgggaacaag taacccgtat cggtgaggat caccggaata tccgggcgaa
     1021 tttgattcac cagatgcagg ctcaccgccg cctgaatgcc aaagctggaa gaaagtacat
     1081 attcaccggg cagattatcc agcgcccagg ctacgcggcc ttcagcgtcc agtttttcca
     1141 gttgggcgtt agtttccgcc agcgccagaa tgcgatctac cttcggcagt tcgttcaggg
     1201 cgtttagatc gagtttggac atagattcct cactgtttgc cttgcctgat gcgacgctca
     1261 cgcgtcttat caggcctaca agaccgggct gatggttaat cccacaaatc gcgcgccgga
     1321 tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct
     1381 tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat ttccggctcg
     1441 gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc
     1501 aggttgtagc gacccggcgc tttacccacc aggcccactt ccgccagcat cgcgcgacca
     1561 caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac accatgtttc
     1621 gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctccgc ttccgccatc
     1681 gccagcgggc aagtcgggaa cgagacgcag gccattgagt tttcacgctg cggcgtgacg
     1741 gcattcatta acccgctctc tttggcgatc ttctcgatct tcgctttctc gctttccggc
     1801 acaccggcga tgatcagatt ctggttcgcc gtaatgcgga aatcaccttt gtggatcttc
     1861 gcgatctcca gcaggccggt tttcagcgga cgccccggat aatcaaggat gcgaccattt
     1921 tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc aatacgatct
     1981 ccgcgtccgg tgaactcata cggacggatc ggttcaaatt tgatccccgc gcgacgctcc
     2041 acttccgctt taaacgtctc aacccccacg cgctccagcg tgtatttggt tttggcattt
     2101 ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc cgccactgcc
     2161 agcgtatgct ccagcggcag atagccaaac tcactcgccg tgcgggcgta ggttttcttg
     2221 ttgccgtgtt cgatagaaag tccaccgccc accaacaggt taaagcccac cagcttgccg
     2281 ttttcggcga ttgccacgaa gttcatgtcg ttggcgtgca gatcgatatc gttctgtggc
     2341 gggatcacta ccgtggtttt gaatttacgc ggcaggtagg tctggccgag gatcggttct
     2401 tcatcggtgg tggcgacttt ttcctgatcg agccagatct ccgcatacgc gcgggtacga
     2461 ggcagcagat gttcagagat tttcttcgcc cactcgtacg cttctgcgtg cagctgcgac
     2521 tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagcggt cgccagcgca
     2581 tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt cagaatgccg
     2641 tggaactgga atgtctggcg gttggtcagg cgaatgctgc catagatggt gttttcaccg
     2701 gcaaatttat cgatcgcctg ccactgtttg gtggtgataa ctccacccgg cagacggcag
     2761 cgcaacagca tcgcatggcg cggctccagc ttctgttcag cacgttcggc gcggatgtcg
     2821 cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttatc gcccttaaag
     2881 ccgccggtca ggccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta gttgctttca
     2941 agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc tggatgtttt
     3001 tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca cttaaaaatt
     3061 catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga agtgcctgct
     3121 caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca ccgtcattga
     3181 tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc ttgtacgtaa actttttctt
     3241 tttgatcgcg cgaccaggca agatcgatac gcgtcagcac gccctctttg acgtaacgct
     3301 gccactccac ctggtagagg aaatcttccg taaagtgcgg gttgccaaag aacagccagt
     3361 ttttacccgg cgcttcgtca gcggcgcgct gctgcataaa ggcgcggaac ggcgcgatgc
     3421 cggtgcctgg gccaatcata atcaccgggg tttccgggtt agcgggcagg cggaagttat
     3481 cgttatgttc gataaatacg cgaacttcgc cctcttcttc cacgcggtcc gcgaggaagc
     3541 tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca ccaacggtga
     3601 cgtgtacttc gttctcgact tcctcctgcg aggaggcgat ggaatacaga cgcggcgtca
     3661 gcgggcgcag cagattaatc agcgcttcgg cgtccagttg cgccggagag aagcgcacca
     3721 tgtcgacaat cggcgtagtc gcggcgtaat gctgtaactt cgctttatcg cccaccagcg
     3781 gcaacagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatattg gcggtgttga
     3841 cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg ccctcgacgg
     3901 tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc agtgccggat
     3961 cgttctgata ccagacaccc agcgcgtcac ccggctggta acgcaggccc gagtcaccta
     4021 agtcaatttc gatatggcga acgtcttttt cagagttacg cccggtaatt ttctggttaa
     4081 ccgaaaggct cgctaccagc ggcgcgtctt tgctgtacgg gctggtgtgg atttcattta
     4141 ccacgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat
     4201 caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacgtcg gcgtcgacac
     4261 ggtcgagcag gcgttcaccg cccagttccg ccagcttgct gtcgaaatct ttcccggact
     4321 ggcagaaaaa ttcataagaa ctatcgccga ggctaaacac ggcaaaagcg gtattttcca
     4381 gcttcggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct tccggcggtt
     4441 ccccttctcc ttgcgttgac gtcaccacga taagcagttt ttcgctggcg atttgtttga
     4501 atttatagtc gcccgcgttc accagcttaa cgttcagttt tgccgtcaat aaatcatcgc
     4561 gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga agcagagata atagttatac
     4621 ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc agcaggctgc tgattgagca
     4681 cgccccagaa atagccagaa acccaggcaa gctgggtggg agttaaatcg gtcgtggccg
     4741 cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt gggacctgtg
     4801 tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt cagcgcccat
     4861 aaaacagaag agaaggtaag gttaacgggg tgaacggtgt ggattaaaga cgggatagcg
     4921 ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa caaaatagat
     4981 taaccaacct aatgaaaaac aaatgaattt agccaatcat taagataaat cagcgatttt
     5041 gcgcaacaaa gtcgttttag ataatgcgaa aaaagagcct ttccggtact ctacggcggt
     5101 tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt tcaccttcga
     5161 agccgctcac cgcttaccac acgtcccgga agggcataaa tgtggtcgcc tgcacgggca
     5221 ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg gctggattat
     5281 cgatttcgct gaactaaaag cggcgtttaa gccaacttat gagcgcctcg atcaccatta
     5341 tctcaatgat attccaggtc tggaaaaccc aactagcgaa gttttagcaa aatggatttg
     5401 ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag aaacctgcac
     5461 cgctggttgt atctatcgcg gcgaatgata agagtgtggc ggcggtcaat ttcccttaag
     5521 taacgctatg ttagggtgtt gtgttcagga tatctggggc atgacatgga agacgactgc
     5581 gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg ctgcgcgcga
     5641 gcgggtttat ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa aaatctttcc
     5701 ggcgggcggt tatataccca tgcactcgcg gaactcctcc ctcaatttca tctgaccgcg
     5761 cctcttgaac gacgcatcac tcacgaaagc ctttccctgt taacgccgga ttgcgcaacg
     5821 acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc acggttcgat
     5881 ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccctgg tgcgacggtg
     5941 gatgcactgt atgaagaaaa cggcagggtg tgtggtgtca tttgtggtga cgatattctc
     6001 cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga acgtcacggg
     6061 ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt gctgtcgctg
     6121 gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc agcgttgctg
     6181 ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat ttctttatac taatcaacaa
     6241 acgctctcgt tagggattgt ttgcccactc tcttccctta cgcaaagtcg tgttccggca
     6301 agcgagctgc ttgctcgctt taaaacgcat ccggcagtgc gcccgcttat caaaaacacg
     6361 gaatcactgg agtatggtgc gcatctggtg ccagaaggtg gcttgcacag tatgccggta
     6421 caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg cgtcaatacc
     6481 ggaatttctg tgcgcggcat ggatacggcg ctaactggcg cgcaggcggc ggcacaaaca
     6541 ctgataagcg cctgccagca ccgcgagccg caaaatctgt ttccgcttta tcatcacaac
     6601 gtagagcgca gcctgctgtg ggatgttcta cagcgttatc agcatgttcc ggtgcttttg
     6661 caacgcccgg gatggtaccg tacgtggcct gcgttaatgc aggatatttc ccgcgattta
     6721 tgggatcagg gtgataaacc tgttccaccg ctgcgccagt tactctggcg tcatttacgt
     6781 cgtcatggct tgtggaatct ggcgggcgat gttatcagga gtgttcgatg tctgtagccc
     6841 gtaatctctg gcgcgctgct gatgcgccgc acattgttcc ggctgactcc gttgagcgtc
     6901 agatggtaca acggttgatt aacgcctgtc cggtaggtct tttttcgctc acaccggaag
     6961 gtgacttacg cgttgattat cacggctgcc tggagtgtgg cacctgccgt ttgctgtgcg
     7021 acgaatccac actacaacag tggcgctatc cgccttccgg attcggcatc acctaccgat
     7081 ttggataaat aataaggata atttatgccc ctcttacacc tgctccgcca gaatccggtg
     7141 attgctgccg ttaaagataa tgccagcctg caactggcca tcgattctga atgccagttt
     7201 atttccgtct tgtacggcaa tatctgcacc attagtcaaa tcgttaaaaa gattaagaac
     7261 gctgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa taaagaagtg
     7321 gtgattcagt ttttgaaact ggtgaccgag gcggatggaa ttatcagcac caaagcatcg
     7381 atgctaaaag cggcaagagc ggaaggtttt ttctgtattc atcgcctgtt tattgttgat
     7441 tcgatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga ttgtattgag
     7501 atcctgccag gctgcatgcc caaagtgctg ggctgggtga cggaaaaaat ccgccaaccg
     7561 ctgattgctg gcgggctggt atgcgatgaa gaagatgcgc gtaatgcgat taacgcgggt
     7621 gtcgtggcgc tttccaccac gaataccgga gtctggacgt tagcgaaaaa attgctttga
     7681 cgggataatc gtaaccaatt gaatttggtt tgatttgtag gccgcacgcc acatccgaca
     7741 ttcagcgcct gatgcgacgc ttaacacgtc ttatcaggcc tacaggtccc cagtccagaa
     7801 ccgtaggcag gatcatccgg cagcacgtta ttggcattct gcgtcaatgt ttgtcaccag
     7861 tgcctccagc atcactttcc agtcatcaac cacgccaaca tctgcctgtg aaaataccgc
     7921 tgcgctggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc ccgccattaa
     7981 cgctgctgcg ccagaagcgc caacaacaat acacacctca ggtgccagca gatgtccgga
     8041 aataccaatc actttttcag catcaacgcc accgttcatc actcgcgccc gactgtagcc
     8101 cacttcggct cccagctttt atgccagcat agcgatttcc tggttatctg cctctccgcc
     8161 ctgccccacc accaacaccc gtctggcctc agctaatggg tcgcgggtaa catttttcag
     8221 gttctctatg ctaaccagcc agtccggaag cgcacctggg actatgttca gttgctgcat
     8281 accagagggc aacgtggcat ttttatctgc tcccgcctgg cgcgccaggg aaagacatag
     8341 cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt ccccagtggg atttgcgcac
     8401 gcttacgatt ggaatctcta acgaagtcac ctggcagatg cttgcaccat gtaaacgcca
     8461 ggccagccga gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa caacgttcgc
     8521 aggcgttcgc tgccactgtt ccaccagcgc atccagcacc tgttccacca ctacgggctg
     8581 tggttctatt tgccagtgcg ccagggtaca accgctgaaa tcctgcgccg ccagccagcc
     8641 cgctaatgcg gcattctcct gatttattgt cacaattgcg atattcatgg ctgcatcctt
     8701 tggcgtaaat agtcctgcca tagcttttgc gctttttctg ctaccgtttg cccatcaatc
     8761 agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat cgctggcaat
     8821 tcggcagcaa cagttttacg gataatttcc gctttccccg cagccattcg ctggcgcatt
     8881 cccggtactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc aggcaagcga
     8941 atccgacaac agcgcagccc atgttcagta cgctgttcga gggtgataaa cggcgcgtcg
     9001 agagtgaaac gctccacctg ggtgaagcaa ggccagccca gcatttccgc cagcaaaaat
     9061 ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag atccagcgga
     9121 ttctgacgct gccattcggc aatatggcga gcgacaaatt ccgacgcaaa gcgcagatct
     9181 gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata gcgcagccag
     9241 tgcaacgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt accgtttttt
     9301 ctctgcgcca acagcagcgc ggcggcagcc tgttcatcag caccgagtaa acttcgcagt
     9361 agcgaaatat ccggtccgct attaccatga gccgccgcct gccactcttt ttccgccagc
     9421 attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac ctcctacatt
     9481 tcccgccgcc accagcggga gtgctttggt ttctggtgcc catagccagg ttacaacaaa
     9541 accagccaac aacactgccg ccaggagcaa taaggtgact tgcattcccc actgcgccag
     9601 cacccacggc agcaggccag tacttaccgc cgcccccaac cgactcatgg cggtggcaaa
     9661 accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa tgcccaccag
     9721 attactgact gccgaaatgg tggtgctgaa gagaacaaaa gcagcagcgt taatgaactg
     9781 ccggaaggca aacaggccat cactaccagc gttgccgcca gcagcaaaaa acttcccagc
     9841 aaaaatttgc gatgtgccag caggtgcgtc agaactaatc ccagcagcgc gcccacaatt
     9901 aacaacgcat taagcatcag gctggcagtc agcgcatctt ccagtccaat agtctgggcg
     9961 atagttggca gccaggtata aatcacaaac catgggatta cgaggcagac aaagaagacg
    10021 ctgttaaacg ccgtgcgccg ccagtagcgc gaagagaaca aggttttgat gtgtttatgg
    10081 gtcgccgttg ccacttcatc gcccagtaaa acatggggac caaaatagcg atgcacgata
    10141 gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc gtggcgattc tggcgttccc
    10201 cagcgtaata acgtaatcaa caacgcgggc agagctgccg atgccagcag ccagcgccag
    10261 gcctccgggt tttcggaaat aaagtgatgt ccggcaatac ttgccaacac atagcctacg
    10321 gtccacacca cgctgaatgc gcccagcaaa acaccgcgat ggcggcgcgg ggaaaattca
    10381 gccagcaagg tgtgacctac tgagtaatcg cctcccagac caatgccgat caaaatgcgc
    10441 agtccaataa gatgctctgg cgtggtggca aaaaattgca agaacgaagc gagcgtaatc
    10501 agcaaaaagc tgaaggtgaa gattttttgc cgaccaatat ggtcggagat ccacccaaga
    10561 accaggctgc caagaaacaa accaaggtgc gccgagccgc cgatcattcc cgccataaac
    10621 ggcgtcagtt gcatggcggg cgtaagttga ataatggcgt aaccaatgac gccgagaaca
    10681 taaccgtcgg tcaggtgtgc gccgaaagtg agcgcagcaa ttcgacagtg aaaacggtta
    10741 agcggtaaat catccattcg caccggtgaa gtgttcatct ttaccccttc attgtccggt
    10801 aataagaacg gcttctcgcc tgagaagccg aaaaggcaac gccccatcat cgcggaacgc
    10861 tgcctgacac attatttttc aatcggatag atagtacccg tattcataat gccattagga
    10921 tcgaactgct ttttcagccc ttccagcaac gcccacgcgc tgccgtgttc cagcttgctc
    10981 cagtgaacgc gatgtttacc gataccgtgg tggtgcacca tcgaaccacc gaggcgaatg
    11041 gtttcttcgc aaatgatttt gttgagcgga ttgtggtact tgtcgatttc ctcttccggc
    11101 ttacagttga caacgttgta gtcgtagacg aagtacatgt tggtgccgtt ctgatagcta
    11161 tgagaggagt gaccgcccag catggtgatg tcgtcggcgt gcgggaactc ggtacgaata
    11221 cggttaataa cgctttcgta gatttcgtgg atgcagctcc agcagccgga cacttcggta
    11281 gtaaagccca tgttgccggt tttgaggatc tgcacacgtt cggcagccac tttatccggt
    11341 ccccagttca ggttgttaaa ccaggtttcg atcagcttgc tgtccacgcg ttggcattgc
    11401 gggtagcggg caacgatttc cgcaatccct tcgcccgtcg ccttcgcaat gcgagggtta
    11461 ccttcagcca taaagatcag cacgcatttt ccgtcggcaa aatgggtgaa gtgttgggtg
    11521 ccatcttcag cgtcatacaa acgagcgatc gacggacgat acccttccac catgatttca
    11581 cgcaggatgt tgaagccggt tttcatgtct tccaggatat agccgtagaa gaggttgttt
    11641 tccggggtga atttaaagat tttcactgtt acttcagtga tatagcacaa tgcaccttcg
    11701 ttgccgatga tgatgtgacg aatgtccggg ccagccgcgc ggcgtggcac gtttttaatg
    11761 cgtgtgacgg tgccatctgc caacactgcc tccagaccaa cgaccatatc ttcgattgcg
    11821 ccgtagagtg tggagaactg cccgatactg cgggttgcta ccaggccgcc catctgcgcc
    11881 agcggctttg actgcggaga atgccccgtg gtgtaacctt tttcacgcaa cgcgttttcc
    11941 agcacctcca gcggaacacc acattgcgcc gtcgcctgca tattctcaat atcaatatta
    12001 atgatttgat tcatggcgga accgtcgagc accaccgagt tttctacaac agtttccagc
    12061 ccaccttcgg tggcggaagc accggtacgc ggcacaccgt taattttgtg cgcattcata
    12121 aaattcagca cacgggatac ttgctctgtg gaaccgagtt ttacgaccgc tgccggaatc
    12181 ggcaaagtat aaatgccatg aatatccgga aatttacgaa aacggtcaat actgttcttc
    12241 tttaatactg tttcatcggt aattacgcga tctgcaccaa caatttcctt tagctggtcg
    12301 acaatcgctg cgcgagataa agacatagta attccttcct gataataaaa atggaataat
    12361 cattaataag agaaaacaga aaccaccgtt atgggttagc gcactaaata accgccatcg
    12421 accactaata aatgcccgtt gacataattc gatgcctgac ttgcgagaaa cacggctgcg
    12481 cccattaaat cctgagtatc gccccaacgg tttgccggaa tatgatcaag aacgcgctga
    12541 ttggtttctg gattactgcg tgtcgccagc gtaatgtctg ttgcataata gccaggggcg
    12601 ataccattta cctgaatatt atattgacct agttcatcac aataagcttt ggtgaatccg
    12661 gcaagagcat gtttagtagc agaatatgca ggtgaccatt gtccacctaa gtaagagaac
    12721 aatgaacaga tattaatgat tttaccgctt ttctgcggga tcataatttt tgccgcttcg
    12781 tagcttaact cgaatgcggc ggtcaggttc acatcaatca tcgggtccca gtcggcacga
    12841 ccgaagtcca gcaccttatt cagcttacaa ataccggcat tgttaaccag aatatcaact
    12901 gtaccgaaac gctcacagca ggcagcgata atcttctgcg gcgcgccttc tgcggtgata
    12961 tccacctgca tgaagtccac ctcaacaccc tgattttcaa tcatttcctt tgtttcgccg
    13021 ttatctttga caaaacttgg aataaagaca tttgcgcctg ctttggccaa cgccatggca
    13081 aatgcctggc ctaaaccgct attcccgccg gtaacaattg cggttttacc tttcagggag
    13141 aaaaaatcca ttgagaacgc attgagagat tcgattgaca tagttagctc caatttccgt
    13201 aaggcaaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt tctcattatc
    13261 tctggtaact ggagataatt tttagcataa ggacataact gctaatgtga tcgtaatcac
    13321 agtgtgatat tcgtattatc aaatagtttt aaaaagcaga agcgtgatta ctctcacaca
    13381 acacctctgc tttccatgtt atttattatt tcaattacta gagacctgag aaaagtaatc
    13441 cacctgaaag ggcggcttac ttttctcttt tttttgtcta ttacaaatgg gaatacgata
    13501 tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat aattagtttt agcggcggcg
    13561 ttagtttcga cctggcttat ttacgttata tttatcaaat tcccatggcg aaatttatgg
    13621 gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg gctattattc
    13681 tttatgcccc cagcggcgtt attgccgata aattttcaca ccgcaaaatg attacttccg
    13741 cgatgatcat taccggatta ctgggtctgt taatggcaac gtatccaccg ctgtgggtaa
    13801 tgctctgtat tcaggtcgcc tttgcgataa caacgatttt aatgctgtgg tcggtgtcga
    13861 ttaaagccgc atcgttgctt ggcgatcata gcgagcaagg gaaaattatg ggctggatgg
    13921 aagggctgcg cggcgtcggt gtaatgtcgc tggcagtgtt taccatgtgg gttttttctc
    13981 gctttgcacc ggatgacagc accagcctga aaacggtcat tatcatctat agtgtggttt
    14041 acatcttgtt ggggattctg tgctggtttt ttgttagcga taacaacaac ctgcgcagtg
    14101 ccaataacga agaaaaacag tcattccagc ttagcgacat ccttgccgtt ttgcgtatca
    14161 gcaccacctg gtattgcagc atggtgattt ttggcgtctt caccatctac gccattctga
    14221 gttactccac caactatctg accgaaatgt atggcatgtc gctggtggcg gcgagctaca
    14281 tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg cccacttggc ggcattatca
    14341 ccacctacag taaagtgaaa tcccctaccc gcgtgatcca aatcctttcc gtactcggcc
    14401 tgctggcgtt aactgccctg ctcgtcacga actctaaccc gcaatcggtc gcgatgggga
    14461 ttggcctgat tttactgctg ggattcacct gttacgcctc acgcgggctg tactgggcct
    14521 gccctggcga agcgagaaca ccgtcttaca ttatgggcac cacggtaggt atttgctcgg
    14581 tgattggatt cctgccggat gtcttcgttt acccaattat cggccactgg caagacaccc
    14641 agcccgcagc agaagcctac cgcaatatgt ggctgatggg catggcggcg cttgccatgg
    14701 tgattgtctt tacctttttg ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa
    14761 tggccagcag caagtaagcc aggaaaatta caggagaaaa atcatgtcga agaaatacat
    14821 catagggatt gatggcggaa gtcagagcac aaaagtggtg atgtacgatc tggaaggtaa
    14881 cgtggtttgc gaaggcaaag gtttattgca gccgatgcac acgccagatg ccgataccgc
    14941 agaacatcct gacgacgatt tatgggcatc attatgtttt gccggtcacg atttgatgag
    15001 tcagtttgcc gggaataaag aagatattgt cggtattggt ctgggatcca tccgttgctg
    15061 ccgtgcgtta ttgaaagccg atggcacgcc tgctgcgccg ttgattagct ggcaggatgc
    15121 acgcgtaacg cgtccctacg aacacactaa tcctgacgtg gcctatgtca cctctttttc
    15181 gggttatctg acgcatcgct taaccggcga gtttaaagac aatatcgcca actattttgg
    15241 tcagtggccg gtggattata agagctgggc atggagcgaa gatgctgcgg taatggagaa
    15301 gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg ccaggcactg ttcttggaca
    15361 gatcacacca caagccgcgc tggcgacaca tttcccggca gggctgccgg ttgtttgtac
    15421 caccagtgat aaaccggtag aagctctggg agccggatta ctggatgatg aaacggcggt
    15481 gatttcactc ggtacttata tcgcgctgat gatgaacggc aaagccttgc cgaaagatcc
    15541 ggtggcgtac tggccgatta tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg
    15601 tattcgcaaa ggcatgtgga cggtgagctg gctgcgcgac atgttaggcg agtcgttaat
    15661 tcaggatgcc agggcgcagg atctttcacc ggaagattta ctcaacaaaa aagcttcttg
    15721 tgtgccacct ggctgtaatg ggctgatgac ggtgctggac tggctgacca atccgtggga
    15781 accgtacaaa cgcgggatta tgatcggctt tgattccagc atggattacg catggatata
    15841 tcgttcgata ctggaaagcg tggcgctgac gctgaagaac aattacgaca atatgtgtaa
    15901 tgaaatgaat cactttgcga agcatgtgat cattactggt ggcggttcga acagcgatct
    15961 gtttatgcag atttttgccg acgtgttcaa ccttccggca cgacgtaacg ccattaacgg
    16021 ttgtgcaagt ctgggggcag cgatcaatac ggcggtaggt ctggggctat acccggatta
    16081 cgcaacggct gtcgacaaaa tggttcgcgt gaaagatatc tttataccga ttgagagcaa
    16141 tgccaaacgc tacgacgcga tgaataaagg tattttcaaa gccctaacca aacatactga
    16201 tgtgatcctg aaaaaatcgt atgaagtgat gcatggggaa ttggggaatg cggattcgat
    16261 ccagagctgg tcgaatgcgt aagtgaggaa gaccgggcgg gaaactgccc ggcctgaaca
    16321 tacctgaatg gttatccccg ctggcgcggg gaacataagt tcgacaactt aaaaaatgat
    16381 aacaagtccg gtttatcccc gctgatgcag ggaacataat aaaaacttta actagttcgg
    16441 tttatccctg tttttacaag gaatattaac tacactcgat gtctttagaa taaaagacaa
    16501 tacgaatcgc actttatcaa ttttcactta aagtatgaac tggatatctt tattcataag
    16561 aactattcat caacagcgta tagaggcggt tatgtaaaac cactcattag cctcaaaaca
    16621 aagacaaatt tccagattta ttacttaatc aatgaataac aagattaaaa aacaaactat
    16681 taatctaaaa aaattaatag acattaattc aatttaaaaa aaataataga taaaatttct
    16741 taatggatta agtatcatac aaataacact ttaattaaat ttgatttttt gtatcgataa
    16801 ttgtgaactt ttcatatatg gagagctatt ttgaacatga ggggttacgt ggatatgttg
    16861 cttattacga gtactgctaa tataaaaact tgagaaagag ataacgggtt atatggtggt
    16921 ttatccccac tggcgcgggg aactcctcga ctttaatttt ttcgctgccc gcttttgcgg
    16981 tttatccccg ctggcgcggg gaactctcta aacataacct attattaatt aatgattttt
    17041 ta
//
LOCUS       DABSYT020000096        16944 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.096, whole genome shotgun sequence.
ACCESSION   DABSYT020000096 DABSYT020000000
VERSION     DABSYT020000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.096"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            11..379
                     /gene="acpS"
                     /locus_tag="ID725_RS18750"
                     /old_locus_tag="pgaptmp_003750"
     CDS             11..379
                     /gene="acpS"
                     /locus_tag="ID725_RS18750"
                     /old_locus_tag="pgaptmp_003750"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGV7852252.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTSHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            393..1154
                     /locus_tag="ID725_RS18755"
                     /old_locus_tag="pgaptmp_003751"
     CDS             393..1154
                     /locus_tag="ID725_RS18755"
                     /old_locus_tag="pgaptmp_003751"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGV7852253.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            1158..1706
                     /locus_tag="ID725_RS18760"
                     /old_locus_tag="pgaptmp_003752"
     CDS             1158..1706
                     /locus_tag="ID725_RS18760"
                     /old_locus_tag="pgaptmp_003752"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HGV7852254.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            1728..2009
                     /locus_tag="ID725_RS18765"
                     /old_locus_tag="pgaptmp_003753"
     CDS             1728..2009
                     /locus_tag="ID725_RS18765"
                     /old_locus_tag="pgaptmp_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGV7852255.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            2045..3205
                     /locus_tag="ID725_RS18770"
                     /old_locus_tag="pgaptmp_003754"
     CDS             2045..3205
                     /locus_tag="ID725_RS18770"
                     /old_locus_tag="pgaptmp_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HGV7852256.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            3279..5837
                     /locus_tag="ID725_RS18775"
                     /old_locus_tag="pgaptmp_003755"
     CDS             3279..5837
                     /locus_tag="ID725_RS18775"
                     /old_locus_tag="pgaptmp_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HGV7852257.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSHARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSEATLPETESNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            5842..7065
                     /locus_tag="ID725_RS18780"
                     /old_locus_tag="pgaptmp_003756"
     CDS             5842..7065
                     /locus_tag="ID725_RS18780"
                     /old_locus_tag="pgaptmp_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HGV7852258.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMTNMQAGRAK"
     gene            7062..8015
                     /locus_tag="ID725_RS18785"
                     /old_locus_tag="pgaptmp_003757"
     CDS             7062..8015
                     /locus_tag="ID725_RS18785"
                     /old_locus_tag="pgaptmp_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGV7852259.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            8026..8733
                     /locus_tag="ID725_RS18790"
                     /old_locus_tag="pgaptmp_003758"
     CDS             8026..8733
                     /locus_tag="ID725_RS18790"
                     /old_locus_tag="pgaptmp_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGV7852260.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            8717..9373
                     /locus_tag="ID725_RS18795"
                     /old_locus_tag="pgaptmp_003759"
     CDS             8717..9373
                     /locus_tag="ID725_RS18795"
                     /old_locus_tag="pgaptmp_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852261.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            9374..10684
                     /locus_tag="ID725_RS18800"
                     /old_locus_tag="pgaptmp_003760"
     CDS             9374..10684
                     /locus_tag="ID725_RS18800"
                     /old_locus_tag="pgaptmp_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGV7852262.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGGEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            10693..11481
                     /locus_tag="ID725_RS18805"
                     /old_locus_tag="pgaptmp_003761"
     CDS             10693..11481
                     /locus_tag="ID725_RS18805"
                     /old_locus_tag="pgaptmp_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HGV7852263.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            11478..12866
                     /locus_tag="ID725_RS18810"
                     /old_locus_tag="pgaptmp_003762"
     CDS             11478..12866
                     /locus_tag="ID725_RS18810"
                     /old_locus_tag="pgaptmp_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852264.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            12880..13821
                     /locus_tag="ID725_RS18815"
                     /old_locus_tag="pgaptmp_003763"
     CDS             12880..13821
                     /locus_tag="ID725_RS18815"
                     /old_locus_tag="pgaptmp_003763"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGV7852265.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMFNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            13818..14804
                     /gene="ycdU"
                     /locus_tag="ID725_RS18820"
                     /old_locus_tag="pgaptmp_003764"
     CDS             13818..14804
                     /gene="ycdU"
                     /locus_tag="ID725_RS18820"
                     /old_locus_tag="pgaptmp_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGV7852266.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            15171..16373
                     /locus_tag="ID725_RS18825"
                     /old_locus_tag="pgaptmp_003765"
     CDS             15171..16373
                     /locus_tag="ID725_RS18825"
                     /old_locus_tag="pgaptmp_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7852267.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
     gene            complement(16560..>16944)
                     /locus_tag="ID725_RS18830"
                     /old_locus_tag="pgaptmp_003766"
     CDS             complement(16560..>16944)
                     /locus_tag="ID725_RS18830"
                     /old_locus_tag="pgaptmp_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852268.1"
                     /translation="FINCQEHETNNIHSAMINNLLQEVDIALGKINDIIDSRNISAPH
                     ELANILTREKILTTREKKGNLISLFDGFTLFHCVGMITFLIHYLRTPEEKVENIFMLY
                     GADKNNKLRRRLIYDALGIIQSQQE"
BASE COUNT         4283 a         3799 c         4445 g         4417 t
ORIGIN      
        1 aacgagactc atgagaatag ggacagatat tgttgaaatt gccagaataa ccaaagccat
       61 tattaatgga gagatggctt tttatgagcg tgtttatact tctcatgagc gcttactcat
      121 taaccctgca gaacctgatt atgaacgggc ctctggattc tgggcggcga aagaagcgtt
      181 agttaaagcg attggttgtg gttttcgtga tggcgtacgt ttccaggata ttgaaataaa
      241 gcatgatgca gccaaagcac cttatttcgt tatttccgga agactacaag aaatttttat
      301 cgggcagggg ttgcaaaata actccctgag tatttcgcat tgccgtaccc acgccattgc
      361 gactgtcatc gtttattaac tcaacatatg tgatgaatat gtacaaatat aatgatttaa
      421 atgaaaaaac cgtactggtg acaggagcca gtggtgatat tggactgggt atttgcgaaa
      481 aatatctgga gcagaactgc gatgtttatg cgctctataa atccaacgac gtgcaattaa
      541 cggcgctgaa agcaagccat cctgcaggcg ataaactgca tattgtgcag tgcgatcttg
      601 cctgccccca gagcgtgagc gcattgtgcg aacagataga gcgccaagct ggcaagattg
      661 atgtgctggt aaataacgcg ggcatcgtta aagacagcct gtttgcctcg atgagttatg
      721 aggatttcac ccaggttatc gaaaccaaca tgttcagcat tttccgtctg acgaaagacg
      781 cgctgatgct gcttcgtgcg gcagaaaatc ccgccattat taacgttgcc tctattgcag
      841 cgctgatccc aagcgtcggt caggcgaatt acagcgcgtc aaaaggggcg atcctaggct
      901 tcacccgcac gctggcagca gaaatggcac cctggggcgt gcgcgtcaac gcggtcgcac
      961 cgggaatgat tgaatcgaaa atggtgaaaa aagtatcccg cgcagtggtc cgtgcggtaa
     1021 ccagtaccat cccgttacgc cgtctgggta aatgtgaaga agtggcgaac accatcgtct
     1081 tcttaagttc ctctgcctcc agctacattg tcggccagac catcgttatc gatggcggcc
     1141 tggtcatgcg gtgatttatg tctaaataca cgattccttc gaaaatcttc ctcgaaatgg
     1201 gcggctggcg tcagccgctg ctgatggtcg ataaaattgc agattataaa tacggtgaaa
     1261 atggctttgt cagtgtcgtg aaacacgtca cctataacga accgtatcta ctggggcatt
     1321 tcccggaaga tccgattatg cccggggtga ttatttcgga aatcttcggc caggccagcg
     1381 agtatctctc tttcctgacc gacatttgcg atatctggcg cgaacgtttt gaggaagagc
     1441 tgaagtcgtt acgtgatatt cacgcacgca ttcaccgccc tgaaatgctg gagattatcc
     1501 gtacccggcg cagccaggta cgcggcgtgc tggcggctca gaacctgaaa tttaaagata
     1561 tcgcctatcc gggtgattct attgatgttg tcagcaaatt ggctttttcg gatgccagtg
     1621 gctttaaaca ctattccgtg accgcgtacg tcggtaaaaa gctgattagt cagggcacca
     1681 ttatcaattt ccgtgaaacc aagtaatcca ataagagagt aaatattatg tctaccatga
     1741 atgacgctat tgaacagcgt aaagaagttc tgatgaccat taaaactgag atcatccagc
     1801 gactgaatat tcaacgcgat gaagcgcaaa ttgatgatga tactgcgctg tttggtaatg
     1861 gttttaaact ggactccgtc gatgcaacgg aaattgtcgt tctgctggat aaagtatttg
     1921 atatcaagat cagcgaaagt gacgacccgt cctatatgcg cagtatcaat agtctggcca
     1981 ccttcgttat tcaaaagaaa aacgattaac tacttattaa agaattaatt atcaggacac
     2041 gtttatgaaa tctcttgctg aatttcactt gaaaaataat gcggtaatgg gggtatataa
     2101 caaccgtacc ctaccttctt cttatcacga tgctatgacc gaatataaag ccgttcgtga
     2161 aaacgcgctg ctggtggact attctcacct ctctattgtt agcgtcatgg gcgatgatgc
     2221 ctgggcgctg atcaaccagc tggtttccgc agatgtctcc atcattcgtg atgagcaggc
     2281 aatctactca ctggtgttga atgaagaggg caccattcgc ggagacgtct atgtgctgtg
     2341 cagcattgac ggttattacc tgctttcaga agatatttcg gctgccgaat tgatcgccag
     2401 catgaatacc attctcgaaa aagcggaaga actggatatt caatcgatgc ctgaaattca
     2461 ggatatgcgg gaaaacaact ggggggcaat cctgctcgaa ggcccatact cctgggagat
     2521 catgtctgaa attcatggct ttgacgtgat tggcctgcct tactacgaat acatgaatac
     2581 cgaggaggat ctgctgctct tccgctgtgg taaacacggt gagtatgcgt acatgaccat
     2641 cggtgagcag gcgaaactgg cggagcagtg ggaaaaactg ttaaccgttg gcgagaaata
     2701 cctgatgcag accggcggcc tggattatca aaaaatcgta cgcctcgaaa atccgtgctg
     2761 ggatgccagc ctctgggaag gtcaggcggt gaatccggtt cagttgcaga tgcagtgggc
     2821 ggtgcagtac gacaaagatg attttattgg caaggacgcg gtgacagagc tatcccagga
     2881 gtataccggt aataaactca tcggcatgat cgcacaggaa gaatgcgaag gcattgaggc
     2941 tggcgatcgc gtgctggtgg aaggtcaaga cgtgggctat gtcgtgaagg cactcttttc
     3001 cccagcgttg cagcgtttca ttgccctgac cctgctggaa aaggattacg cctggtctga
     3061 catcagcggc tacgaaattc aaactgctca cggaattatt ccggcgcaat caaaatgtat
     3121 gccgtttatt tataacctga gcatgttggt tagcccaact gagcacagtt atatcgacgc
     3181 gtcaaaaaat aaaagcgcag cgtaatgaat atcgccgcgt gactatagct tacgcggctt
     3241 aaattaaccc caattggcgg gattgccagg tattattcat gaaaacgaac aggaaaagag
     3301 ttgttattac cgggctgggg attttgtcct cactggcgga aaatattcag gatttccggg
     3361 aagcattatt aaataaaaaa aatggcgtta aggatagcga acgctttgca aaatggtttg
     3421 aaaatgcgcg tgcggcagaa gtgttgcatg acattgaata ctccgatctg aacgaagatg
     3481 ttatcgattc actggataac gcagcgctct gggcctacaa agtgggtaaa gatgcgctga
     3541 gtcaggccgg cctgatggat agccgtgaca ccctcaaaga aacaggcatg atcgtcggtg
     3601 tttcgtccgc gggaacagag gcatttttgc cgctgttcga acagcgcatg gaagatttct
     3661 cgctgcgtaa agcactcttt tccggcgcat tctcatcctg ttgttccagc gtttcaacgc
     3721 tgctgggctt acagggtggg gttgaactgg tggcgaccgc gtgtaccgcc agcccgaacg
     3781 ccgtggggat ggcctttgac tacatccaga atggtaaaag caaaaccatg ctggcagtgg
     3841 gcactgagcc catctatctg ccgacctttg ccggattcta cgcactgaat gtcatgcatc
     3901 cagacacttg tacgccgttc tcgggtcagt ccggtatgtc gattggtgaa ggcgctggcg
     3961 ctatcgtgct ggaagagtat gagcacgccg tagcacgcgg ggcgaccatt tatggtgaga
     4021 tcctctctta cgccacttcc tgcgatgctt tccatgaaac cgggccagat ccgcgtgcca
     4081 gcggagcggt gcaggtcatg aacaaggcaa tggcaaatgc gggcgtaacc ccggagcaaa
     4141 tcgattacgt caacgcgcac ggtacgggta ccgaagccaa tgaccgtatt gaaacgctgg
     4201 caatgaaaaa ggtcttcgct aaccacgata aactgctggt gagctccacc aaatcttact
     4261 ttggccacaa cattggcgca gcggggattg tggaactgat tgcctgtctg gcaacgcttc
     4321 cggataaccg cgtactgccg acgctgaatt tcagccatgc acgcccgaat tgcgatctgg
     4381 actatgtgcc gaatgagttt cgcgatcgca aagtgaacat cttcatgaag aataactatg
     4441 ccttcggcgg caacaactgc tgcatgatcc tgagcatgga gcccgcgtcc attccggtca
     4501 gtacctatga ttcgaagcgt gtggctatct cgggcgtcgg tgcagtatcc gcaataggcc
     4561 acacgctcaa ccaggttctg gaaaacatct gggcgcagga ccaccacgtt gaactgggta
     4621 gcgtaacctt cccggacgat acactggaag aagccaaaga gctgctggcc gtgctggatg
     4681 aatcaaacca gtttgccgag ctgtttgatg aagagtacag cttcagcgaa gctacgcttc
     4741 cggaaacgga aagcaacttt aaaacgttcc aggtttccgg gctggagccg cgtaaacacc
     4801 tgcgtcgttt cgatcagcgt aaagcgaccc gtggcggcac gtttgccctg atcgcactca
     4861 ccgaagcgct ggaacaggcg aagcgtaaaa tcaaacgcga cggcgatgag ttgggcatga
     4921 tcatggggat gtcccgcggc ccgcaggaaa ccacctacaa atatctgcaa agtctgaaac
     4981 ccgatccacg caaagtacgt acatcggaat ttcctggctc gctgatgaat gcgatcccaa
     5041 cgttctgcgg gatctctgaa gggatcaaag ggtataccac tacgcttgcg acaggggaaa
     5101 actcggcgct gggcgcgctg acctacggtt atgaaatcgt ccggcagaac ctgcaaccgc
     5161 aggtattggt tggcggcgcg gatgagtact tcccgtcgat gtcgctgtat atggatgcgg
     5221 tgacacaaaa gatccacatg acggctgagg cgagcgacta tcagatttac ggtaaagatc
     5281 cgcaaggtta tgttccgggc gaaggggctt gcatgctgct gctggaagat ccgcagttgg
     5341 ctgtggcgcg tggcgctgag gttctcgcgg aagtggtggg ctacggcaag tcatgcagca
     5401 atacctactt cgacgcctca ttgttggaag aaaaatccgc gtcgatggcg ctggccattg
     5461 aacgcgccct tacgcatgcg ggcatctctg ctggcgatat tgatttggta tgtggtacca
     5521 gcaacggtag cgatgaccat tctcgtatcg aaattgacgc gatttacaac accttcgccg
     5581 cgcaaaaccc ggcggtgccg gtagtgaact ataacgcttg ctttggtttt gtcgcctctg
     5641 ccgctggttt gcttaacctc gccgtgatta ctgactgcat taagaagcag gcagtacctg
     5701 cgattccaaa tacggtggaa ttctttgatg agcgtgtgaa ttttgtacgc cagtcgatga
     5761 aacttgcgct gaaccatgtc ctgcttgttg gcgcaacgga aggcggcaac tactacgcgt
     5821 tcgtcattaa gggataagac tgtggacaat gccgctatta tttcgggtta cagcgtgtgg
     5881 atgccgtatg ccgaagactg ttcgcagtta attgacaatc tgaaacaggg gaaacgcgtt
     5941 gcgcgtacac cctggtttac ctccaacgag gaagccatta aatgcgggtt taagggtaat
     6001 ccgtcagtgg cgacgttgaa acaggtggac gatagcgcat tggacctgct gtctcagttg
     6061 atcgacgaag ccctggaaca ggcaatgctg gataagcatt gcctggccgg gcgcaacgtt
     6121 cgcgtttatc tgaccggtat tgggccgcgt atcgacgggc tggattacaa atctttctat
     6181 aattacaacg atgtagaaga tatcaactta acgcaatcca tcacaaatct gcatgcctca
     6241 aagatgtcgc aggacacgat ttcaagccat cttgcgcgca aatatcgctt gcagtatttg
     6301 ccgcctaaca tgaactgcac cagtaactcg tcactgactg cggtgcatct ggcgacgcag
     6361 ggcattgagc agggcgggat tgatctcgcg attgtgctga actgttcgaa aattaaaacc
     6421 caggacatct ggttcctgga aacgcaatcg atgctggaca gcgaacaagt gcagccgttt
     6481 ggtgaaaaca gtaaaggtgt ggcgttcgca gaaggtttta gcgcgctcct actggaaagc
     6541 gctcatcacc gccgggcgcg tcaacaaagt gagggcgtaa gggtgcaaac gacttacacc
     6601 caaatcagcg cgggccgcag caacgatgcc tcctggctta gcactaacgt gcacaaggtc
     6661 atgcaggccg cgatgaagca ggctgaaatt gcgctggatg atcttgcagc cattcttcca
     6721 cacggcaacg gttcggcagt gagcgataac gcggaggcca aagccatcgc catgttcgca
     6781 ggggagcgac agatccccgt tctcgcctat aaagggcaga tcggctatac cgcaaccgga
     6841 tctggtgttg tcgatctgat cattggccac cattcgttga cgcatcatca actgatcgca
     6901 cctgttggca acgacgtcat tatcgacagc atggcttcgc tggtacttac ggacggcagc
     6961 gtgacaaacc atagcaaacg ccatttgctg aaggttggtg tgggtgtcga tggttcagtt
     7021 attggcgttg tcatgacaaa tatgcaagcg gggcgcgcga aatgacggaa aagagtatct
     7081 acctgtcttc ctgggcgctt acggaaccgg acgacatttt ttatttttat cagccgacct
     7141 ggctcaacgc ctgggaagca catatgtacg aaaggggatt atacggcacc cgtgctaatg
     7201 gctggggact gaagcgcttt ggcgtggaga acgatattta tgcaccagaa cggatccgta
     7261 atccacaata tatgccttac cttgaggcga tggagcaggt gaccgattct gcattacaac
     7321 aaatgccgac taaagaacgc atgaagttaa tgaaatcgcg cacggcattt atttatgccg
     7381 attcctgggg cgaatccggt tcgtttgaaa aaatttccag tgcgctgcat atcgccacta
     7441 ttgatacgct tcccaagaat ttacttaaga agttttcgat aacggaaccg acttgcaaga
     7501 ttcgcggcga aaaacaggca ttcgttcagg cgctgcgtat ggcgcaggat tatctcgcct
     7561 gggatgtatt cgataacgtg gtgatttgtg ctgcctatcg ggcaattccg gtgctggtat
     7621 tctctgaaga agatgtgggt aaaccgccta agcgtttatt tccccgtcgt gctgatgagg
     7681 tgaatttatc tgttgaacga gtgggttgtt ttattctcag ccagcgtgag agcgccatca
     7741 ggctgcaatg cggggaatat cattccggca ctgcgccaca gcatgctacg gatattgatg
     7801 tcatggcctt cagcgaacgg caacaaatgc cgtcccggcc tgctgctggc gttatcgacc
     7861 tggtggccca ttacggtgaa agcggttgtc tgacgcccgc gttgagtatg gattatttaa
     7921 cccgtcatat tcagcccggt ggaaaaatgc gtacggtcat tgccgataaa cagtttggtt
     7981 atcactactt tgaccttgaa taccttgaag gataacgaca gtcgtatgca atctactatt
     8041 aaatcgttgc ccgctatttc gcttagcaat atttataagt gttatggcgc tggggcagga
     8101 aaggttgatg cgcttaaaag cattaatctt gatatcaata aaggcgaatt tctcgcactg
     8161 tgtgggccgt caggcagtgg taaaagtact ctgttgaata ttctctcggg tattgataaa
     8221 ccgaccagcg gaacggtgat atttttaaat aaattactga accaactgcc agaagagcag
     8281 ttggctgtga ttcgtggaaa acatttgggt tttatttttc agttttttaa ccttatgccg
     8341 gtattaaatg ttttcgataa cgtttatttc ccactggtgc ttaatggtca gttcagtaaa
     8401 aaagaagcca ctgaacggac gctgcattat ctcgatagtg ttggattaag cggttttggc
     8461 gatcgtaaac ctgggcaact atcgggcggt caacaacagc gtgtcgccat cgccagggca
     8521 ctggcccacg aaccgatggt ggtcattgcc gatgaaccga cgggaaattt agacctggca
     8581 accggcgagg ctattttgga cctgttgttg acgattaatc agcagaccgg caccacattt
     8641 gttatttcca cgcactccag cgaattaaaa gcgcgggcac ggcgagtggt tgaaattcag
     8701 gatggagtat tgattcatga ctcagatgca taaaggattt ttgctcatcg gcgtcttttt
     8761 gtacgcggcg tttgtggctc cgaagattta cgcggcaaca cccgacgctg ttaaccaggt
     8821 tctgcaacag gggctcccgg cgcagcctgc cgcgctggcg acgctgaccg aggcgttaaa
     8881 gaaagagtat gaagccaggc acaatgtggt ggcgctggtt ttttacgcct atggcttact
     8941 gcgtcaagcg gacggttatg cggcaacaaa cgattttatc catgcctcag aatacgccaa
     9001 aagcggcttt ttctggctgg atgaagcggt ggatttgcag gagaaaaatc aacgcgtacg
     9061 gtatttacgc gcccgcgtag atgcctattt acccgcagac tcagggcgtt gcgtggtgac
     9121 agtcaaagac accgaacaca tgctggctga cccggcgata tggacgacaa cgatccgcga
     9181 ccatattctg gcgatgcgtt atcgcgcatt acgtcactgc aaggacacca cccgcgcgaa
     9241 tgcgttgttg gcgcaaatta aagggcaaaa cgccgcgctt gcgcagtcgc tgacgcagga
     9301 ttttaatgtt gtaccagaat gggatagtga agagttaacc caggtactgc tgccgctgat
     9361 gaaaggtgag taaatgtccg gggaaatttt agctgttaat tgcgttctgc tgctgattct
     9421 gttttttgtg ttttatcagc ttctgtttta ttcacgagat gtcaaatttg catttttgaa
     9481 tctctttcgc cacaagcgtc gctctttttc taccataacc gctattgtat tgggtggcgt
     9541 ggcaattttt ttatatggcg gatttattga ttactctttc tggatcctga aagagcagac
     9601 gattcgtacc aatattggtc atgtacaaat ttacaatcag cactattttg aaacgtcgaa
     9661 taaaaataag agcctgattg ccgattatgc ggcgttaaag aaagctattt tgtcggaccc
     9721 gacgctggcg gcggatattt ccacgctctc cgggcaactg gagtttactg gcgtaatttc
     9781 acattacgag agcgaaacct ccagctattt ctccgcgctg gggggcgagc cgttacccgc
     9841 tttaaagctg ggttcgttcg ataagattat cgcggggagc gatctttccc gggtaaaagg
     9901 tgatgaaatc accctgggca gcggtctggc gaaaacgttg aatgcgaaat atgacgactg
     9961 gctggatgtg atggtggtca acacggcggg cgggcagggc gcattgtcgc taaaaatgcg
    10021 tggcattttt gagtcaggca ttaaagatta tgacgatgtc gcgatgaaga tcccgctgga
    10081 caccgcgcag cgcatgatgg gtacggatgg cgtcagcaaa gtgcttattc tgcttaaaga
    10141 ggatgacacc gcggcgttta gcgcaaagct gcgtcaattt atcgaacgca atcatttgcc
    10201 gctggtggtc aaagactggc gtgatgtttc actcttttat caacaggttg aagggttgct
    10261 atcggggatc tatttcttca tcaagctgat tgttgcgctg attgtcatct tcatgatcgg
    10321 caactcgatg gcaatgaaca ttgttgagcg cacccgtgaa attaccactc tgcgtgccat
    10381 aggcctgaag ccgcttcacg tcacgcgctt gtttctgaca gaaggcattt ttattggtgt
    10441 gattggcgca gtcggcagcc tgctggtggg gggcgtgtta gcctggatta ttaatcttta
    10501 tggtattgcg atgccgccat cgccgggaca aaccatcggc tataccgcct ttataaaaac
    10561 cagtaacccg gaattgattt gggtgacggt ggtattacct attttgacag caacaggtgc
    10621 ctctgtgtta ccggctttac gtgcttcccg gctgaatatt tctgacgcat ttaaattctc
    10681 atagggctaa ttatgaatat ggctttttac ggcaagtggt ttgcttgcct gtggctcgct
    10741 acgtcgtgcg ttcaggcggc gagcacagat aataaggcgc tggagataat tcgtcgcgct
    10801 gacgaaattc gttcgccgaa taaaccgttt cgttacacgc tgacggtaac ggaatataaa
    10861 gcgggcgcaa cccagccaga gaataaacag gtcctcgata tttccatgcg ctttatgaaa
    10921 ccgcagggta atgaaaaagc ggatgcacgc tcgctggtgc gttttattta tccgccgcga
    10981 gataaaggaa aaatcatgct ttccgactgg tacgatttat ggttttacac gccggaactg
    11041 cgacgtccta tgcctatttc acggcaacaa agattaattg ggcaaatctc taatggcgat
    11101 gtcattgtca ctaactttga atatgcctat gactcgaccc tgatggggga agtcacgtgt
    11161 gctgaaaagc agtgttacaa gctggcgctg gtgcgtaaat cggcggatat cacctggccg
    11221 aaggttatct attacgttga aaaagacggt gataatcgcc cgtggaaggc ggcctattat
    11281 tcgcaggatg accagttaat aaaagaagtg ttgtatcagg atttccagcc ggtgttgggg
    11341 aaaacgcgac ccatgaaaat aaccgttacc gatgttcgtc acggcaataa ctattcagtg
    11401 atggagtaca gcgatgttcg tctggaatcg ctgccggagt ttcatttcac caaagagtat
    11461 attcagcgag gtgcgaaatg aaacgtacgc tgaagatctc ttcgttgctg tgtgttgcgc
    11521 tgccgttgac ggtacaggcg gattgcctgt caggtgacga ggtggcacaa aatagcgatg
    11581 tgacgcttta cgagcaggtg tcttatgtca ataaacaggc ttcggcctgg cagattgcag
    11641 gcaaaaatcc ctacacccgt cacaacggtt atcaggaggc ggggattggc atcaatagcg
    11701 gttgttcgat aatcgacaat acgctggacc ttaagctcaa tctgtatgga ataaacgaat
    11761 atgcgcttaa acccgcaggg aaattcgaaa cggatgatag ccgcacgcgt gcgttaatta
    11821 accgcctcag tctggtctat agcgcctcgg acagcgttca atttgaagca ggcaaatttg
    11881 ccgcgccgtc aggaatgttc tttttgcgtt cgccttctga tttgcaaacg cattattaca
    11941 ccggattcca gtcgacgcgt ttgcacgatc ccaaaatgac atcggcgtat caggcgtcat
    12001 cgtggggcgc aaaaatgagc gtggatacac gcgattatgc gttttcggcg agtgtcatac
    12061 cgaagttagc caccatcgac aaacgctatg tgacgtctgg caactggtcg gctaatcagc
    12121 agggaaacag cgacgaagcc tatctgctga gcttcagcga tcatcgcttt ggtgagcaca
    12181 cgccgacagt caatgtgcgg ctcggaccat cgccgtcact cgcgctgtct gacagttatc
    12241 actacacccc gcagcttacg ctgaatgtcg acgcagccta ccaccgctcc cagcaatggc
    12301 ggcatctttc ccaccgtgag acggcgcagg tcgaggaata tcagtttccg gattcgctct
    12361 atgaaacgaa agatgaaagc ggcgtagagc tggcgctggg tgggcagtac accagtgata
    12421 atttcagcgt gtttggcgtt gagtattact ttcagagcga gggttattcg cgcgctgaac
    12481 agcgtcagca gcgcgagtta attgacttcc tcaataccac aaccggctat gcgccactgg
    12541 atcaggcatt tgattcttac aaatacctga tggcttcaga aatcagcaat accgccaatc
    12601 aggggatgct gcagggtaag cactatctga acgcctgggc cagtctgccg ctggcgggag
    12661 agtcgacgct gcagccttcg ctggtggtga atctcgttga tggcagcacg ttgctgggcc
    12721 tgcattattc cacgccgctc agcgctataa gtaaccagct tgaagcctat gccggtggct
    12781 atagcgcgct gggaagtcgg tactccgaat ttgcgctttt tggcgacacg ttaggccttt
    12841 atttgggctt taaatactat ctataaggat aaagagatga tgaataatgc ttcacaaccg
    12901 acagtactgc tttttgccgg tcagggtaat ccggttattg gtatgggcag cgatctttgg
    12961 gatctcaacg ccagtacaaa acatatctgg gattgtgcca gcgatatttc cggactgaac
    13021 ctgcgtcgcc tgtgtcagaa agggccgatg aatcgcctta ttcaaacgac ggtacaacaa
    13081 ctggcggtga ccgcaattaa tgtaacgctc tataccctgt gccgcgagcg tcttgagaat
    13141 ttaacggttg tgggctcctg cggacacagc gttggtgaat acagtgcgct ctacgccgct
    13201 ggagctattt ctcttgagac gctatttggc atgattcact tcagagccag cctgatgcat
    13261 gacgaaagcc agagaaataa aggcgcaatg ctggcggtta aaggcatcaa ccgcgaacaa
    13321 ctgcagacga tgattacgga atcaggcatt gcgctggata ttagctgtga taacacgccg
    13381 cgccagcagg ttattggggg aacgcaggct gcgttgaatg aatttgccac gctgctgatg
    13441 gccgctggat acgaaccggt taaactcggt gtgagcggtg cctggcacac gcgtttgatg
    13501 gaagacggcg ttcaggcaat gcgcgattat cttgccggac tggatatcgc ttcgccagag
    13561 catcaggttc tgatgaacgt aacagcaaaa agcgaggtcg ctccgtcaat tatcaaagaa
    13621 aatctctcat tacacttaac tcatacggtg aaatggactg aatctctcga tacatttctg
    13681 aatatgccaa cctcggtagc tttccttgaa ataagtaata agccctattt ggggaatatg
    13741 ttcaacgatt tcgctggcgt ggatcagcaa cgagttatgc attgtcgtaa agcatttagt
    13801 gatgcgaagg tattcaaatg attcctgatt atttaacttt tattcgcttt caggataaac
    13861 gaagtctgat atacatttat gctattggac ttattctgat aggcttttat tggaaaaatg
    13921 caggatttac ttttccatca gaggatattg gtgtcgttag tgggattctg gccctggtgc
    13981 tgtataattt tatttttgat ctcaaggcgt actgggctta taaatgcgtc acgaagaata
    14041 tcgatttttc gtggtttaag aaaaagcaga accacaaaat agaattattt cttacgcaac
    14101 ctctggtggc aggatttctg tcgttaatca tgttgagtgc aatgagttgg gggctatacc
    14161 agcgtctacc ctcgttatat gcgctgttcc tgatttcgtt acttgggccg ttggttatct
    14221 ttctgctgtt tcggatgatc cgcaccagtt atgtcaagca ggtcgctatt tcagtagcga
    14281 aaaaagtaaa atataaaagt ctgactcgct atgtgctgct ttcggtgtgc atctcaacgg
    14341 ttgttaacct gcttactatc agcccgttgc gtaacagtga ttcttttgtg atagaggggc
    14401 agtggttaac gtttaaatcg ataattgcat tgctcattct ttgtggcgta gtgttggcga
    14461 ttaatctgtt ttttctgcgc ttctccaggc ggtacgcttt tttgggcagg ctttttttgc
    14521 aggaaatcga tctctttttc tccagtgaaa atgcattgtc gacctttttt gccaagccgc
    14581 tttggcttcg gttattcata ttgctggtta ttgaagtgat gtggattacg ctggtgtcgg
    14641 tattggcaac gcttgtggaa tggcggattt ggtttgaagc ctatttttta ctctgctatg
    14701 taccgtgctt aatttactat tttttctatt gtcgattcct ctggcataac gattttatga
    14761 tggcatgtga catgtatttc cgttgggggc attttaataa gtgaggaagt gataggaagt
    14821 gaccagataa tacatatatg ttctgtactc tcttgcgcat tttgattgtt gactgagcaa
    14881 ccagacagtt gatgtgcacg atttcccctc gcccaaacag acgtgggcgg gggcacccgg
    14941 tgtgtgcaat atacgaaaaa aaagcccgta ctttcgtacg agctcttctt taaatatggc
    15001 ggtgaggggg accgcctgaa ataaatctga cttaatccag gggtgttata taatttcatt
    15061 ataaacaatg tattacactt tttgttgtcc gatattatct aatgtattct tagtgaagcg
    15121 agagactaat ggcatactgg atggcatacc agagtgacgg gaattagcgt atggcagtat
    15181 tgacggatac gaaagcaaga catatcaaac ctgatgacaa accattgccc catgggggaa
    15241 ttaccggact gacccttcat ccttcttcag taaaggggcg ggggaaatgg gtttttcgtt
    15301 atgtaagtcc ggtgacacaa aaaagacgta atgctggatt gggaacttac ccagaggtca
    15361 gtattgctga agctgcacgt actgcccgga taatgcgaga gcaacttgct gcaggtgatg
    15421 atcctctgga gattaaaaag gctgaatctg agaaagtcgc tatcccaaca tttgccgatg
    15481 cagccaggcg tgtacatgca gaactgtctc ctggatggga aaatccaaag catgtaaggc
    15541 agtggttatc gacgcttgag aattacgcgt ttcctcaact gggagcaaaa acgctggatt
    15601 cgattacggc tgcggacgtg gcagaaacac tgcgtccagt ctggttaacc ttgtcagaaa
    15661 cggcaagccg ggttaaacag cgcattcatg ttgttatgca gtggggatgg gcgcacggtt
    15721 tttgtgtagc aaatcctgtt gatgtggttg accatttgct tcctcagcag acaagaggac
    15781 gtgatgaaca ccaacccgca atgccctgga ggcagttacc gctttttgtg gcgaccagtg
    15841 tgtataccga tgaaccttat aatgttaccc gcgcactgtt attaatggtg atacttacag
    15901 caactcgctc gggcgaagcc aggggaatgc gctgggctga aattgatttt cataagcggg
    15961 tatggactat acctgcagaa agaatgaaag ccaggataca gcatcgtgtt cctttatccc
    16021 ggcaggctat ttacattctg gaaaatatac gtggcctgca tgatgaactg gtgttccctt
    16081 cacccagaaa gcagcagatc ctttccgata tggtgttgac aagttttctg cgtaaaaaga
    16141 aagccgtcag tgacattccg gggcgagttg ccacggcaca tggttttcgc tcaacattca
    16201 gggactggtg tagcgaacag gggtattcgc gggatctggc ggaaagggcg ctcgctcata
    16261 cgctgaaaaa taaggttgag gcggcatatc atcgtactga tctactggag cagcgtgtac
    16321 cgatgatgca ggcatgggcg gattatgtga tgtctcaaat tgtgaataaa taatagtgaa
    16381 aatgtatatt gtacgatcat gatttgatat gtgatcgtag cagtagtaat taagaaaccg
    16441 cttttatgtc aaatgtattg agctgataat tatcagatcg tcaggaaagc gtattttgat
    16501 taacagggga ttacttaaca atcccctttt tataaaaaat aatttgcctg tttaactctt
    16561 cactcctgct gagactgaat tattcctagt gcgtcataaa tcagtcttct gcgtagttta
    16621 ttgtttttat ctgcaccata taacataaat atattttcaa ctttttcttc aggtgttctg
    16681 agataatgga taagaaaggt tatcattcca acacaatgga ataaagtgaa tccatcaaac
    16741 aggcttatca ggtttccttt tttttcccgt gttgtaagta ttttttcgcg ggtaagaata
    16801 tttgccagtt catggggggc tgatatgttt ctgctgtcta taatgtcatt tattttacca
    16861 agggctatat caacttcctg taataggtta tttatcatag ctgagtgaat gttattggtt
    16921 tcatgttcct gacagtttat gaac
//
LOCUS       DABSYT020000097        16650 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.097, whole genome shotgun sequence.
ACCESSION   DABSYT020000097 DABSYT020000000
VERSION     DABSYT020000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.097"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            98..715
                     /locus_tag="ID725_RS18835"
                     /old_locus_tag="pgaptmp_003767"
                     /pseudo
     CDS             98..715
                     /locus_tag="ID725_RS18835"
                     /old_locus_tag="pgaptmp_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            818..1321
                     /gene="yfbM"
                     /locus_tag="ID725_RS18840"
                     /old_locus_tag="pgaptmp_003768"
     CDS             818..1321
                     /gene="yfbM"
                     /locus_tag="ID725_RS18840"
                     /old_locus_tag="pgaptmp_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGV7852269.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1388..2845)
                     /gene="nuoN"
                     /locus_tag="ID725_RS18845"
                     /old_locus_tag="pgaptmp_003769"
     CDS             complement(1388..2845)
                     /gene="nuoN"
                     /locus_tag="ID725_RS18845"
                     /old_locus_tag="pgaptmp_003769"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGV7852270.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2852..4381)
                     /gene="nuoM"
                     /locus_tag="ID725_RS18850"
                     /old_locus_tag="pgaptmp_003770"
     CDS             complement(2852..4381)
                     /gene="nuoM"
                     /locus_tag="ID725_RS18850"
                     /old_locus_tag="pgaptmp_003770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGV7852271.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4612..6453)
                     /gene="nuoL"
                     /locus_tag="ID725_RS18855"
                     /old_locus_tag="pgaptmp_003771"
     CDS             complement(4612..6453)
                     /gene="nuoL"
                     /locus_tag="ID725_RS18855"
                     /old_locus_tag="pgaptmp_003771"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGV7852272.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6450..6752)
                     /gene="nuoK"
                     /locus_tag="ID725_RS18860"
                     /old_locus_tag="pgaptmp_003772"
     CDS             complement(6450..6752)
                     /gene="nuoK"
                     /locus_tag="ID725_RS18860"
                     /old_locus_tag="pgaptmp_003772"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGV7852273.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6749..7303)
                     /gene="nuoJ"
                     /locus_tag="ID725_RS18865"
                     /old_locus_tag="pgaptmp_003773"
     CDS             complement(6749..7303)
                     /gene="nuoJ"
                     /locus_tag="ID725_RS18865"
                     /old_locus_tag="pgaptmp_003773"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGV7852274.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7315..7857)
                     /gene="nuoI"
                     /locus_tag="ID725_RS18870"
                     /old_locus_tag="pgaptmp_003774"
     CDS             complement(7315..7857)
                     /gene="nuoI"
                     /locus_tag="ID725_RS18870"
                     /old_locus_tag="pgaptmp_003774"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGV7852275.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7872..8849)
                     /gene="nuoH"
                     /locus_tag="ID725_RS18875"
                     /old_locus_tag="pgaptmp_003775"
     CDS             complement(7872..8849)
                     /gene="nuoH"
                     /locus_tag="ID725_RS18875"
                     /old_locus_tag="pgaptmp_003775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGV7852276.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8846..11572)
                     /gene="nuoG"
                     /locus_tag="ID725_RS18880"
                     /old_locus_tag="pgaptmp_003776"
     CDS             complement(8846..11572)
                     /gene="nuoG"
                     /locus_tag="ID725_RS18880"
                     /old_locus_tag="pgaptmp_003776"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGV7852277.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11625..12962)
                     /gene="nuoF"
                     /locus_tag="ID725_RS18885"
                     /old_locus_tag="pgaptmp_003777"
     CDS             complement(11625..12962)
                     /gene="nuoF"
                     /locus_tag="ID725_RS18885"
                     /old_locus_tag="pgaptmp_003777"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGV7852278.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12959..13459)
                     /gene="nuoE"
                     /locus_tag="ID725_RS18890"
                     /old_locus_tag="pgaptmp_003778"
     CDS             complement(12959..13459)
                     /gene="nuoE"
                     /locus_tag="ID725_RS18890"
                     /old_locus_tag="pgaptmp_003778"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGV7852279.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13462..15264)
                     /gene="nuoC"
                     /locus_tag="ID725_RS18895"
                     /old_locus_tag="pgaptmp_003779"
     CDS             complement(13462..15264)
                     /gene="nuoC"
                     /locus_tag="ID725_RS18895"
                     /old_locus_tag="pgaptmp_003779"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGV7852280.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15358..16020)
                     /gene="nuoB"
                     /locus_tag="ID725_RS18900"
                     /old_locus_tag="pgaptmp_003780"
     CDS             complement(15358..16020)
                     /gene="nuoB"
                     /locus_tag="ID725_RS18900"
                     /old_locus_tag="pgaptmp_003780"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGV7852281.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16036..16479)
                     /gene="nuoA"
                     /locus_tag="ID725_RS18905"
                     /old_locus_tag="pgaptmp_003781"
     CDS             complement(16036..16479)
                     /gene="nuoA"
                     /locus_tag="ID725_RS18905"
                     /old_locus_tag="pgaptmp_003781"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGV7852282.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         4117 a         4706 c         4339 g         3488 t
ORIGIN      
        1 aaccgtaata aaacaaacac tcataactca ttataaataa tgataaaata acgtctttta
       61 ttgataattt acatatgaaa aaaattttcc ttttgttata ttatttctgt tttcactccc
      121 tctgattatt ttttaccaac cctgggtcac tgcattatcg ccaacgcccc gacacgtaat
      181 tcctgagcaa ttagaaaaaa cagttcgtta tcttacacaa actgtgcatc cacgtaatgc
      241 cgacaatatt gataatctga ataggtccgt agagtatata aaaaaaggtc tttgtcagta
      301 gcggtgccag agttaccgcg cagggcgtcc ccaattggct attacgactc tgcgcctggt
      361 agccaggatt acccttaccc gcaatgtcct ggctttatcc cgatcggggg gactttattg
      421 cctggtcggc agaatacagg atatcaacgc cgttcgtcag gtaaaaacgg cattgttatc
      481 atcccgggat ttatctgttt attctatgaa tgccccatgg tttattcacg gtattgattt
      541 ctctgaccac cttaattatt ggcaacacga tattctcgcc gtaatgatta ctgacaccgc
      601 tttttatcgt aataaacaat accacttgcc cggtgataca gcagacagat tgaattatca
      661 gaaaatggct cagatggtgg atggtgttat aactttgtta cacaacagta aataataaaa
      721 atttctcgag gcgggtccag aaacagcgac atgtacattt tattttccct cgtctgtttt
      781 aagatgcgaa acataaaatt caatggagga aaaataaatg ggaatgattg gttattttgc
      841 agaaattgac tcagaaaaga ttaaccaact gcttgagtct actgaaaaac ctttgatgga
      901 taatatacat gacacgcttt caggattacg ccgactggat attgataaac gctgggattt
      961 tttacacttt gggctgacag gcacctctgc gttcgatcca gcgaagaatg atccgctgag
     1021 tcgtgccgtg cttggtgaac acagcctcga agatggtata gatggtttcc tgggattgac
     1081 atggaatcag gagttagccg cgacaatcga ccgactggaa tcactggatc gaagtgagct
     1141 gcgtaaaaag ttttccatta agcgactcaa tgaaatggag atttatccgg gagtgacctt
     1201 ttcagaagag ttagaggggc aactctttgc ttcgatcatg cttgatatgg aaaagctgat
     1261 tagcgcgtat cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta ttgttgggtg
     1321 atggtgtcag ggcaccaaaa aaaaccgccg aacatgtcgg cggctttctg acttacaaac
     1381 tgatagctta catcagcggc attgctaacc gcacaatact aatcagcggt tgtggccata
     1441 cacccagcac cagtaccaac agtgcagaaa tcagcaccac gataccgccc gcgctgtact
     1501 gccagtttga cggtgcatcg cgacccggtt gttccggtgc gtgcaggtac aggctcaccg
     1561 ccacgcgcag gtagtagtag aggccgattg ccgaaccgac aaccacggca cccaccagcc
     1621 accacaggtg tgcctggaca ccaaccgcca gcacgtagaa cttaccgata aagcccagcg
     1681 tcatcgggat accggccaga gacagcatca tcaccgtcat caccgccgcg aggatcggac
     1741 gatgccagaa cagaccgcgg taagagaaca gggaatcagc atccgggcca cgatacgggc
     1801 tggacatcag gctgaccacg ccgaacgcgc cgaggctgct gaacagataa ccggccaggt
     1861 aaacccctac cgcttccatc gacatctcgc cagtttgcag cgcaatcagc gccaccagca
     1921 gatagccgag gtgagagata gatgagtaac cgagcagacg tttgatattg gtctggctca
     1981 gcgccatcag gttaccgaag atgatggagg caaaggcgat aatcgccagc accacccgaa
     2041 tcgcttcgct gtcacccacc ggtgcgtaga ggaacagacg catcaccaca ccgaagatag
     2101 cgattttgct cgccgtcgcc aggaaagtgg aaaccggcgc aggcgcgccc tggtatacgt
     2161 ctggcgtcca caggtggaac ggcaccagag agagtttgaa gccgaggcca acaatcatca
     2221 ggccgaaacc tgccagcaac agcggctcgt ttagcatacc gtcgccgagg tttttaccca
     2281 gcgcgacaaa cgacaggtcg ccagactgcg catacaccag cgccataccg aacagcagga
     2341 aagaagacgc tgcggcagaa aggatggtgt atttgatact ggcttccagc gaacgtttct
     2401 ggcggaaagc gtaaccgacc aggccaaaca gcggcaaaga gatcagttca ataccgagga
     2461 acagagacgc cagatggttt gcattcgcca gcaggatccc gcccagcgcg gcaattaaca
     2521 ccaacaggta gaactcatcc ttgttgtcgt tatagccttc aagccacggg taggcgaaag
     2581 tacaggtggc gaggctcgcc aacaatacca gcccggtgta aagcatggcg aaaccatcaa
     2641 cgcgcatcag cggcgtaacg tccatagcgc ccgcctggcc aacaaaccag agcgaaacca
     2701 gcgccgcgtt aagcccaata accgagagcg tagcgttgag gaaatgattg cgtcgccacg
     2761 caatggagag catcacaacc accaccgtca agccgacgat cagcaacggt agcagtgcga
     2821 tcaggttttg tggagttatt gtcatggcga tttacggcct tgtagtagta acggaattaa
     2881 caaaccactg ctggatattg ccaatcgcgg agtgcgaggt atccagaatc ggctgcggat
     2941 agaagcccag cagtaccagc agcaccacca gcaacaggat cataaacagc tcacgcagcg
     3001 acatccctgg cagttcctgg ctggcaatct ggcttttcgc tttaccgaag taagcgcgat
     3061 gtaacatcgc cagcgaataa acagatgcaa agaccagccc aaaggtagag ataacggtaa
     3121 tcaccgggac aacctggaag ctgccgaaca gaatcataaa ttcgccgacg aagttaccgg
     3181 tgcctggcat cccgagcgtt gccaccgcaa agaacagtga caaggctggc agccatttca
     3241 tcttgctcca cagaccgccc atcatgcgca tgtcgcgggt atggatacgt tcataaagct
     3301 gaccacaaag aataaacaga cccgccgctg acaagccgtg cgcaatcatc tggattaccg
     3361 cgccctggta ggccaactgg ctgccggtgt agatagcaat cagtacgaag cccatgtggg
     3421 aaaccgaggt gtaggcgatc agacgtttga tatcggtctg ggcgaaggcc atccacgcac
     3481 cgtagaagat gccgataaca cccagccaca tggcgattgg cgcgaactct gccgacgcgt
     3541 ttgggaacag cggtagggag aaacgcagca gaccgtaagc ggcggttttc agcaagatcc
     3601 ccgcgaggtc cacggaaccg gcggtcggtg cctgtgagtg cgcatccggc agccagccat
     3661 gcagcggaac caccggcatt ttcaccgcaa acgcgatgaa gaagcccagc atcaacagat
     3721 attccacacc actggacatt ggcgtattca gcagctcttc atagttgaag gtccagacgc
     3781 cggtcgcatt gtagtgaaca aaaaccagcg ccaggatggc gatcaacatc accagaccac
     3841 tcgcctgggt gtaaatgaag aacttggttg ccgccgtgat acgcgtttta ccatcagagg
     3901 ctttatgccc ccacagtgcg atcaggaagt acatcggcac cagcatcatt tcccagaaga
     3961 agaagaacag gaacatgtcg atggcaagga acacgccgat aacgccgccc aggatccaca
     4021 tcaggttgag gtggaagaag ccctgatatt tttcgatctc tttccacgaa cagagtaccg
     4081 ccagcacacc gagcagaccg gtcagcacga ccatcagcag cgacagcccg tcaatggcga
     4141 gatggataga gataccaaaa cgcgggatcc acggcatgtc gaattcagac tgccactgcg
     4201 gaattccggc ggattgcgtc agtgaataac cgccctgcaa ccacagttgc agcgacagcg
     4261 ccagcgtcaa tcccatggtg atcagcgcga tccagcgcgg caccttgacg ccaaagcgtt
     4321 cggtctgcca gcacaggaag ccgccgataa aggggattag tattaaccag ggtaataaca
     4381 tagctaagta tgtcccttat atttactcct gctcaggcta tctcactcgc cagcccgaat
     4441 cccggcagtg ctcgcaatcc tcacgtactt aagtacgctc cggttgctgt gcgctggcgg
     4501 tatccggtct gacttcgccg atgacgcctg tacttcagga acagttacct aaaatttatt
     4561 cttagttcac agacccggcg caacgccggg tatattcact cagccaacaa ctcaacgcag
     4621 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4681 cagataaccg ttctcgctta acagcaggcc tttacctgca aagcgggaaa ggacagccgg
     4741 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     4801 cttgacgaac actttgtcgt acagccagtc aaagccccag gcgttgtacc accaggtgcc
     4861 cagcagacgg cccggcgcac tgttggcgat ggaggtcacc agagtacgtt tacccagcca
     4921 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     4981 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5041 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5101 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5161 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5221 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5281 tagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5341 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg cgagaatgac
     5401 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgggtcatca agtgaaaaat
     5461 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5521 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5581 cagcgtaacc gccccaacaa tgcccaccag atgcagaact tccggcgtca tcaggaacag
     5641 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     5701 cgcggagaca ggcgtcgggc ccgccatcgc gtcggcaagc catgtctgca acggcaactg
     5761 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     5821 gccgtcagca aagtgcgctg gtgccagttc caccatttcg cggaagttca gggtgcccag
     5881 ttcgttgtaa agaatgaaca gcgcgaaagc gaggaacacg tcacccacac gggtcacgac
     5941 gaacgctttc attgccgctg cgccattctt cggatcggtg taatagaacc cgatcagcag
     6001 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tgtcggcaag
     6061 caccagaacc accatgctgg cgataaacag gttggtgtaa gcgaagaagc gagagtagcc
     6121 ctcttcaccg cgcatatacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6181 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6241 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6301 gtcaacgcca ataaaggcag ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6361 cgccgagacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6421 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6481 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6541 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg gccccagtaa
     6601 cttccggcga ccacaaaggc cagcgcggaa gcgttaatca tgatttccag accaatcagc
     6661 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     6721 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     6781 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     6841 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     6901 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccttgatcgt
     6961 taacaccgag aatggcgtaa acaatcacca ccaacatgat ggctgacaaa attgccggac
     7021 caatccacac ctgcggtttc agccactggc gttcttgttc gatttctgaa ccgcccaggt
     7081 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7141 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7201 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7261 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7321 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7381 gtcgattgcc atacctgcca tccggtagaa gttatactcc gggtatttgc ccggaccgga
     7441 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7501 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacaaac cacagaaaat
     7561 gcagcgtgag aagttgatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7621 tttttgcagc gagatacagc cgaccgggca ggctaccgcg cagaggttac aggctacgca
     7681 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     7741 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     7801 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     7861 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     7921 agcgtcagcg gcaggcagat tttccagccg aaggacatta cctggtcata acgcggacgc
     7981 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8041 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8101 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8161 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8221 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8281 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8341 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8401 gaagcacgca tcgcaccgag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8461 accgcgtaaa ccgccagacc ggccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8521 gcaaccaccc aacccggact gactggcaca atcgcaaagg ccagcagcag cgaggtaaag
     8581 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8641 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccacccca gccaacacgg
     8701 ttaggtccgt aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     8761 gccccgcagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     8821 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     8881 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     8941 tcagcccttc agcgatttca accggcagag tgaccgtgtt gccatcgtaa ctaaaggaga
     9001 cgcgtgtacc tgcgtttaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9061 gctgcggcat acgacactgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9121 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9181 tggtgaagta atccagacca ttttcgctgg tttcaaacag acgcacaccc ggatcgccaa
     9241 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9301 ccggagccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9361 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9421 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9481 gaatacggaa tgtcgcatct ggcgcagcat ctttgatacc cgccagttcc gggattttcg
     9541 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9601 tgctgtgcag cgagtgcagc cagcgccaac tttccagcat gacagttttg ctgtcgtaat
     9661 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
     9721 cgtcgctttc agcaaagctg gcagcggaaa gcaccagatg ggcgttttcc atgatcgctg
     9781 tgcgttgatg gtcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
     9841 tagcagaagc gtgacgatgc agatcgtttt ccagcaccac caccgcgtcg gcgcgtccgg
     9901 tttccagttc ggttaacgct tcttcaagcg atccaccgcc cataatgccc aaccccatgc
     9961 tgttgacgga acgggcaatc atggtgatac cgacgtcagc gccgcgacct ttcagggctt
    10021 tcgcgacgtt agccgcagcc tgaatcacct ctaagctacc ggcgttcgtc ccggagataa
    10081 tcaacggttt cttcgcaccc gccagtgcct gcacgatgac gtcgattttg ctttgcagct
    10141 caggttcaat accgtcaacc gctggcgcag agttatccag cgcatgggcg atggcaaaac
    10201 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10261 gggtgtcatc aacgttggta acaaacagcg gatgcttagc acgttgaccg atattgagga
    10321 ttgccgcaat ctgccagtca gccactttct gtgctgccgc catttcgcgc gctttacctt
    10381 tcaccgcctg gcgcactgcc agcgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10441 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10501 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10561 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10621 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10681 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    10741 gatctttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    10801 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccataacgc tcgccggggc
    10861 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    10921 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    10981 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11041 tgtcatgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11101 cgcagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaacggc cccaaatcct
    11161 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11221 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11281 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11341 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11401 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11461 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11521 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11581 ttccagtttt ctcagtcaga gactgagcgt taatcgaaat tcggttacca gcgctctttc
    11641 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgatcccc
    11701 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac agcaccaggt
    11761 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    11821 atatcgcccg gctgaccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    11881 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    11941 ttacgcacca gcgacaccat gttgatttca tggtcaaccg ccatcgccag cgccgtaccc
    12001 agacggctgc ccgctttacc gatactttcg aattccatcg gcaggtcaag gtgcgcttcg
    12061 gtcaggaagt cggtgcccgc gccgcctggc tgccaggctt tgaatttcag accgtcacgc
    12121 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12181 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12241 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12301 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12361 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12421 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12481 ataatgtttt tgccaagcaa tcccgcttca gtcgcttcgg caatggcgcg gcgcagattt
    12541 actgccgctt caatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12601 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12661 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    12721 gattcgtctt tcggcatcag gctccacttc aagccagtcg aaaagcccgc gccgccgcgc
    12781 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    12841 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    12901 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    12961 atttataccg ctccagcagt tcagggatcg cttccggggt cagattcgcg tgagtgtcct
    13021 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13081 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13141 cctgaatacc ctgataccca ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13201 ggcggccaac cggctggcgg aagatctggc tgtagaacgt tgccacgcct tcgacgtcgc
    13261 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13321 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13381 gcatctcgtg ctcgatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13441 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13501 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13561 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13621 caggtagtaa ctgttgatcc ctttggtcgc ctcaatcatc tggaaggatt cattggcagg
    13681 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    13741 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    13801 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttaag
    13861 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    13921 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    13981 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14041 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14101 cggcatccag tcgaggaact cacgcagcag gcgatcccag ccgcgcggca ggtcgtgcgc
    14161 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14221 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14281 ctgaataaag gtcgagatat acagcaggtg gctgttgatg cggaacagtt cggagagcat
    14341 tacgcgaatg acgttaacgc gagccggcac ggtgatcccg gccagtttct ctaccgccag
    14401 cacgtaaggc atttcgttaa cgcagccgcc gaggtattcg atacggtcgg tatacggaat
    14461 gtagctgtgc caggactggc gttcgcccat tttctccgca ccacggtggt ggtagccgat
    14521 gtctggtacg cagtcgacaa tctcttcgcc gtcgagctgc aaaacgatac ggaaagcacc
    14581 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt tttcggtgcc
    14641 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    14701 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc gccgggtaat ctttacgcag
    14761 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    14821 tgtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    14881 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    14941 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15001 gcggtgcgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15061 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15121 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15181 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15241 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagcaagc
    15301 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15361 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15421 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15481 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15541 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15601 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15661 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    15721 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    15781 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    15841 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    15901 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    15961 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16021 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16081 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16141 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16201 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16261 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16321 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16381 gcctaccagc atcaggcaac ataggccaat ggcaacgata agaaagatag cgaatgccca
    16441 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16501 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16561 aaaaacctta caatcactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16621 cctttctata accttttgtc aactttaaca
//
LOCUS       DABSYT020000098        16619 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.098, whole genome shotgun sequence.
ACCESSION   DABSYT020000098 DABSYT020000000
VERSION     DABSYT020000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.098"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(324..749)
                     /gene="arsC"
                     /locus_tag="ID725_RS18910"
                     /old_locus_tag="pgaptmp_003782"
     CDS             complement(324..749)
                     /gene="arsC"
                     /locus_tag="ID725_RS18910"
                     /old_locus_tag="pgaptmp_003782"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGV7852283.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(762..2051)
                     /gene="arsB"
                     /locus_tag="ID725_RS18915"
                     /old_locus_tag="pgaptmp_003783"
     CDS             complement(762..2051)
                     /gene="arsB"
                     /locus_tag="ID725_RS18915"
                     /old_locus_tag="pgaptmp_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGV7852284.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2105..2458)
                     /gene="arsR"
                     /locus_tag="ID725_RS18920"
                     /old_locus_tag="pgaptmp_003784"
     CDS             complement(2105..2458)
                     /gene="arsR"
                     /locus_tag="ID725_RS18920"
                     /old_locus_tag="pgaptmp_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGV7852285.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3202..3285
                     /gene="dinQ"
                     /locus_tag="ID725_RS18925"
                     /old_locus_tag="pgaptmp_003785"
     CDS             3202..3285
                     /gene="dinQ"
                     /locus_tag="ID725_RS18925"
                     /old_locus_tag="pgaptmp_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGV7852286.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3339..4691)
                     /gene="gorA"
                     /locus_tag="ID725_RS18930"
                     /old_locus_tag="pgaptmp_003786"
     CDS             complement(3339..4691)
                     /gene="gorA"
                     /locus_tag="ID725_RS18930"
                     /old_locus_tag="pgaptmp_003786"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGV7852287.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4763..5605)
                     /gene="rlmJ"
                     /locus_tag="ID725_RS18935"
                     /old_locus_tag="pgaptmp_003787"
     CDS             complement(4763..5605)
                     /gene="rlmJ"
                     /locus_tag="ID725_RS18935"
                     /old_locus_tag="pgaptmp_003787"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGV7852288.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5808..7850
                     /gene="prlC"
                     /locus_tag="ID725_RS18940"
                     /old_locus_tag="pgaptmp_003788"
     CDS             5808..7850
                     /gene="prlC"
                     /locus_tag="ID725_RS18940"
                     /old_locus_tag="pgaptmp_003788"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGV7852289.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7858..8610
                     /gene="rsmJ"
                     /locus_tag="ID725_RS18945"
                     /old_locus_tag="pgaptmp_003789"
     CDS             7858..8610
                     /gene="rsmJ"
                     /locus_tag="ID725_RS18945"
                     /old_locus_tag="pgaptmp_003789"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGV7852290.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8659..10128)
                     /gene="dtpB"
                     /locus_tag="ID725_RS18950"
                     /old_locus_tag="pgaptmp_003790"
     CDS             complement(8659..10128)
                     /gene="dtpB"
                     /locus_tag="ID725_RS18950"
                     /old_locus_tag="pgaptmp_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGV7852291.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10294..10347
                     /locus_tag="ID725_RS18955"
                     /old_locus_tag="pgaptmp_003791"
     CDS             10294..10347
                     /locus_tag="ID725_RS18955"
                     /old_locus_tag="pgaptmp_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852292.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10325..10378
                     /locus_tag="ID725_RS18960"
                     /old_locus_tag="pgaptmp_003792"
     CDS             10325..10378
                     /locus_tag="ID725_RS18960"
                     /old_locus_tag="pgaptmp_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852293.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10445..10879)
                     /gene="uspA"
                     /locus_tag="ID725_RS18965"
                     /old_locus_tag="pgaptmp_003793"
     CDS             complement(10445..10879)
                     /gene="uspA"
                     /locus_tag="ID725_RS18965"
                     /old_locus_tag="pgaptmp_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGV7852294.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11270..11605
                     /gene="uspB"
                     /locus_tag="ID725_RS18970"
                     /old_locus_tag="pgaptmp_003794"
     CDS             11270..11605
                     /gene="uspB"
                     /locus_tag="ID725_RS18970"
                     /old_locus_tag="pgaptmp_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGV7852295.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11676..13175)
                     /gene="pitA"
                     /locus_tag="ID725_RS18975"
                     /old_locus_tag="pgaptmp_003795"
     CDS             complement(11676..13175)
                     /gene="pitA"
                     /locus_tag="ID725_RS18975"
                     /old_locus_tag="pgaptmp_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGV7852296.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13407..14609
                     /gene="yhiN"
                     /locus_tag="ID725_RS18980"
                     /old_locus_tag="pgaptmp_003796"
     CDS             13407..14609
                     /gene="yhiN"
                     /locus_tag="ID725_RS18980"
                     /old_locus_tag="pgaptmp_003796"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGV7852297.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(14927..15979)
                     /gene="yhiM"
                     /locus_tag="ID725_RS18985"
                     /old_locus_tag="pgaptmp_003797"
     CDS             complement(14927..15979)
                     /gene="yhiM"
                     /locus_tag="ID725_RS18985"
                     /old_locus_tag="pgaptmp_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGV7852298.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            16363..>16619
                     /locus_tag="ID725_RS18990"
                     /old_locus_tag="pgaptmp_003798"
     CDS             16363..>16619
                     /locus_tag="ID725_RS18990"
                     /old_locus_tag="pgaptmp_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852299.1"
                     /translation="MKVFHNIFKYISSNHQDKHSDKVNNHQHHGKVDKKHRSEIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKIWFYN"
BASE COUNT         4328 a         4156 c         4271 g         3864 t
ORIGIN      
        1 aaggtatctt ataattattg aataatttgt tgtgtaaagc actgataaat cataatccag
       61 tacgttttta aactgctgca ctgtatcgct gatcactaag cagctcactg atacatcatg
      121 ctggacgcga taattggaat ctcacgcagg gagtaatgtg taaatagcaa aatcgcctga
      181 tgcgctacgc ctgtcaggcc tacaaataac atacaattta ttgaattttc atgactttgt
      241 agatcggata agacgcgtta gcgtcgcatc cgacataaat aaagcgcact tttctaacaa
      301 cctgtggggg ggatatagcc gctttatttc aggcgcttac ccgcttcatc aaccactttc
      361 tcgccatctt ccttggtgaa tgcgcctttt tgcgcatctg gcagaatttc cagcaccact
      421 tctgaagggc ggcacaggcg agttcccagc gtcgtcacca caatcgggcg attaatcaga
      481 atcgggtgct gaagcataaa gtcgattaac cgatcgtcag taaatttatc ttccgcaagg
      541 cccagctcct catacggttc gacgttttta cgcagcagcg cgcgtacgga aatccccata
      601 tcggcaatga gtttgaccag ttcatcgcgc gttggcggag tttccagata atggataatg
      661 gtcggttctg tgccgctgtt gcggatcatc tccagcgtat tacgcgacgt gccgcaggcc
      721 gggttgtgat aaatggtaat gttgctcata tcagtatctc attacaaagt gaaagagaga
      781 cgtagcgcca gcgcagccag cgtcacaaac agcacaggca gggtcatgat aatccctgta
      841 cggaagtaat atccccagct gatagtcata ttcttctgcg aaagtacgtg cagccagagc
      901 agcgttgcca ggctaccaat cggggtgatt ttcggcccca aatcgcagcc aatcacgttg
      961 gcataaacca tcgcttcttt gataacgcca gatgccgtgc tgccatcaat ggacaacgcg
     1021 ccaaccagta ccgtcggcat attgttcata atagaagaga ggaaggcggt gaggaatccg
     1081 gtgccgagcg tcgcggccca caggccgtta tccgccagca cgttgagtac gccagaaaga
     1141 tattccgtta atccggcatt gcgcaggcca taaaccacca gatacatgcc gagcgagaag
     1201 atgacaatct gccagggcgc accgcgcagc actttacccg tattaatcgc atgaccgcgt
     1261 ttagcgacga caaataatat caacgcgccc actgccgcaa tggcgctgac cggaatgccg
     1321 agcggttcca ggacgaaaaa tcccaccagc agaagcagta aaacaaccca gccagttttg
     1381 aacgtagcag gatctttaat cgcttctgcg ggagatttca gcagcgccat gtcgtagttc
     1441 tgcggaatat ctttgcggaa atagagatgc aacatcacca gcgtggcaac aatcgcggcg
     1501 atatccaccg gcaccatcac cgaggcatat tcgcgaaagc cgaggccaaa gaaatctgcg
     1561 gaaacgatat tcaccaggtt ggagacaata agcggcaggc tggcggtatc ggcaatgaat
     1621 ccggccgcca tcacaaacgc cagcgtagtg cctttactga accctaaagc cagcagcatg
     1681 gcgatgacaa tcggtgtcaa aataagcgcc gcgccatcat tggcaaacag ggcggcaacg
     1741 gcagcaccga gcaggacaat ccaggtaaac agcaagcgac cgcgtccatt gccccagcgt
     1801 gaaacgtgca gtgccgccca ttcaaaaaag ccggactcat ccagcagcag gctgatgata
     1861 ataacggcaa taaacgcagc cgtcgcgttc cagacgatat tccacaccac cggaatatcg
     1921 cccggatgta ctacgcccgt aactaacgcc agtactgcgc cgagcgttgc actccagccg
     1981 atgcctaaac ctttcggctg ccagataacc aatacaatgg tcaggacaaa gatagcgcct
     2041 gccagtaaca taatgcctcc cggaaaaaca catgtgcaaa tccatatgtg tttagataaa
     2101 ttttttaact acaagtgttc ttactgtcta cggaacaatt ttgtctggcc aggttgcgga
     2161 caatcacctg aaccttttcc tgttcgcatc gccaggcctg ctcaataatt ttcgcggccc
     2221 atgatggaat atgcggtgat aagcggtaat gaacccactt accctgcttg cgatccagca
     2281 acaaaccgct ttcacgaaga agcgccagat ggcgggagat cttgggctgc gactggtcga
     2341 gagcagtgca gagatcacag acgcataact ctcccagctc gctgagcagt aaaacgatgc
     2401 ccagacgggt ttcatcggca agaattttga acagttggat gggtaacaga aatgacatat
     2461 tgcgctcctg attgttgcag gtagtgtctc tcttcgaagc ggataagtca aaaacatata
     2521 tggtttaacg aatgtgtatg tgcagaggaa ggtaataggt gtgaattaag cgttggctat
     2581 tcatttgaaa ggaggtctga atcaggacgt aaaaaaaccg actttgcgtc ggttttttac
     2641 tttccagccc agagttggtg gctctggctg gagtgagaat gcccacatct aacggttcaa
     2701 tactattagc cagtggctaa cattgcaagc gttagcgatt tcttcagctg gagaagggac
     2761 agtttgctat acatgttggt gaaagaggat ttatatactt tcatcgggtc gctgccgtac
     2821 caggctttgt ccgaatccgg atgtaaaaaa accgacttcg cgtcggtttt tttactcttc
     2881 agccctgagt tggtggctct ggctggagtg agaatgccat catctaacgc atcaatacta
     2941 ataccatact gaaactattg caaggacatg ctggttttat aacctgaata tactgtatga
     3001 ttatccagtt agctctgagg cattttcact ctggcaatgc gcataaacgc tttcaaagtc
     3061 ctgttcagtg gtacgggtgg tgccgttaac tgatgctctg gccggagtga gaataccatt
     3121 atctaacaat agggcatgcg ccgtgacagg cgatggatga gtaagcggat gcattctcac
     3181 tccatcgtat ggagaacacg ggtgattgat aaagcaatca tcgttcttgg ggcgttaatt
     3241 gcgctgctgg aactgatccg ctttctgctt cagcttctga actgatagcg gaaacgtaat
     3301 taagggcaaa gagcacacca ctctttgccc tttaacattt aacgcatggt cacgaactct
     3361 tctgccgccg ttgggtgaat ggcgacggta ttatcgaagt cttttttggt tgcgcccatc
     3421 ttcagtgcca ccgcgaagcc ctgcaacatt tcgtccatac caaaaccaat gccgtgaata
     3481 ccgacaatct tctcttctgg cccaacgcac accagcttca tgcggcacgg ctggcggtga
     3541 gtggtgacgg cggtatacat cgcggtgaaa gaggatttat acactttcac ctgatcgtcg
     3601 ccgtactgct cgcgcgcctg cggttccgtt aaaccaacag taccaatcgg cggatggctg
     3661 aagaccacgg tcggaatgtt gctgtaatcc agatgctcat ccggcttgtt attaaacagg
     3721 cgttcagaga gacggcgacc cgctgcaact gccaccggtg tcagttctac tgcacccgtg
     3781 ttatcgccca ccgcgtaaat accttcaacg ttggtgtttt gatatttatc gacgacgata
     3841 tagccttttt cgttggtttt aacgccagcg gcttcgaggt tgatgttgtc attggcaggc
     3901 tcgcggccaa tcgcccaaat caggcaatcc accgtttcac tgcgaccatc ttccagctcc
     3961 agcgtcaggc taccatcggc atttttcact accgctttcg ggatggcgtt ggtgtgcaac
     4021 tgcgggcctt cggcgttcat cacttcgacc agcgtttcag agatcatcgg gtcgaagctg
     4081 cgcagcggcg catgtttacg cacaaacaga tgcgttttcg cgccgaggcc gttaatcacg
     4141 ccagccagct caacggcgat gtaacccgcg ccaacaaccg ccacacgctc tggcaaagca
     4201 ggaagggcaa agaaaccatc agaatcaata ccgtattcca cgcccggaat atccgggtgg
     4261 ctcggacgac cgccggtggc gatcagaata tgatcggctg tgatggtttc accgttaact
     4321 tccagcgttt tggcatcaac gaagcgggca aagcctttga ttacatcaac gttattttta
     4381 ccgagcacgt tttcatagga agtatgaata cggtcgatat aggcggtacg gctggcgatc
     4441 aacgtttccc agttgaattt attgatagtg gtatcaaaac cataatccgg gccgtacata
     4501 tggatcgctt cacggatttg cgccgcgtgc cacatcactt tcttcggtac gcagccgacg
     4561 ttcacgcagg tgccgcccag ctctttggct tcaatcagcg cgcatttctg accgtacata
     4621 gccgcgcggt tgatggaggc gataccgccg ctgccgccgc cgatggcgat gtaatcatag
     4681 tgtttagtca tgacagagtg tccttatcgt tgattaccgc gattgtagcg cgagacgtaa
     4741 taggtgccag caatggctgc aattactctg gcacgatcca gcttacggtg gcgtgcccgg
     4801 tgccagctgg aaccagtttg ctgtgcagcc acggcagcac gttattcatc tgctgttcca
     4861 gtttccacgg cgggttaatc acaatcatgc cggaagcggt catgccacgg cgatcgctgt
     4921 ctggcagtac cgccagttca atttgcagaa ttttgcgaat gccggtcgct tccaggtcgt
     4981 ggatcatgcg cttaatttgc tggcgcagca ccaccggata ccacaatgcg taagtgccag
     5041 tggcgaaacg tttgtaacct tctgctatcc cgctgactac cgcctgatag tcggttttca
     5101 tttcatacgg cggatcgata aggattaaac cacggcgaga aaccggcggc agtttggcct
     5161 taagctgctg gaaaccgtcg gatttttcga cgcgcgcacg gctgtctttc tgaaattcag
     5221 aacgcagcaa cgggtaatcg ctcgggtgca gttcggtcag ttgcaggctg tcctgttcac
     5281 gcagtaactg acgggcaatc aacggtgaac cggggtagta acgcaactga ccgctacggt
     5341 tgaagtgttt taccacattg atgtacggct ccagctccgc aggcaaatcg tcctgctgcc
     5401 agatacgggc gataccttcg agatattcgc cggtacgctc ggcatgttcg ctgcctaact
     5461 gataacgccc ggcccctgcg tgggtgtcga gatagagaaa cggtttatct ttctctttca
     5521 gcgactcgat gatcaggctc tgaacggtat gtttaaggac gtcggcgtgg ttgccagcgt
     5581 gaaagctgtg gcgataactg agcatgggta aaggtgttcc ggtaagtaaa aatcggcccg
     5641 tattcggggc gcacaaaagc ctatcaggac agtataccga aaagaggccg ccgccgcgaa
     5701 agcgtaacgt ttctcattga aattcactac acttaacccc atgctacaaa cattatgtaa
     5761 agcgcctgtt gagcgcttcc ttaacctctt taaccaggac tgcgctaatg acgaatccgt
     5821 tactgactcc ctttgaattg cctccgtttt ctaaaattct cccggaacat gtcgttccag
     5881 ccgtgactaa ggcattgaat gactgccgcg aaaatgtgga gcgcgtagta gcgcaaggag
     5941 caccgtacac ctgggaaaat ctctgccagc cgttggcgga agtggacgat gtgttggggc
     6001 gtattttctc cccggtcagc cacctgaact cggtgaaaaa tagcccggaa ttgcgcgaag
     6061 cgtacgaaca aactctgccg ctgctgtcgg aatacagcac ctgggtaggg caacatgaag
     6121 ggctgtataa agcgtaccgc gacctgcgcg atggcgatca ttacgccacg ctgaacacgg
     6181 ctcagaaaaa agcggttgat aacgcactgc gcgattttga actctcaggc atcggtctgc
     6241 cgaaagagaa acaacagcgt tacggcgaaa ttgccactcg tctttctgaa ctgggcaacc
     6301 agtacagcaa caacgtcctt gatgcgacga tgggctggac taaactcgtt accgacgaag
     6361 cggaactggc ggggatgcca gaaagcgcgc tggctgcggc aaaagcccag gccgaagcga
     6421 aagagctgga aggctacctg ttgacgctgg atatcccaag ctacttgccg gtaatgacct
     6481 actgcgacaa ccaggtcttg cgtgaagaga tgtatcgtgc ttacagcacc cgcgcttcag
     6541 atcaaggtcc aaacgccggt aaatgggata acagtaaggt gatggaagag atcctcgcgc
     6601 tgcgtcacga actggcgcaa ctgctgggct ttgaaaacta cgcgtttaaa tcactggcta
     6661 ccaaaatggc ggaaaacccg cagcaggtgc ttgatttctt aaccgatctg gcaaaacgcg
     6721 cgcgtccgca gggcgaaaaa gagctggcgc aactgcgtgc cttcgccaaa gccgaatttg
     6781 gcgtggatga gttgcaaccg tgggatatcg catactacag cgaaaaacaa aaacagcacc
     6841 tctacagcat cagcgacgaa caactgcgtc cgtacttccc ggaaaacaaa gcggttaacg
     6901 gcttgtttga agtggtaaaa cgtatttacg gcatcaccgc taaagagcgt aaagatgttg
     6961 atgtctggca cccggatgta cgtttcttcg aactgtatga cgagaacaac gaactgcgcg
     7021 gcagtttcta cctcgacctg tatgcccgtg aaaacaagcg cggcggggcg tggatggatg
     7081 actgcgtagg ccagatgcgt aaagccgatg gttcgctgca aaaaccggtc gcgtatctga
     7141 cctgtaactt caaccgcccg gtgaatggta aaccggcgct gttcactcac gacgaagtga
     7201 tcactctgtt ccacgagttc ggtcacggcc tgcaccatat gctgacccgc atcgaaaccg
     7261 ccggtgtttc cggtatcagc ggtgtgccgt gggatgcggt cgaactgccg agtcagttta
     7321 tggaaaactg gtgctgggag ccggaggcgc tggcgtttat ctctggtcac tacgaaaccg
     7381 gcgaaccgct gccgaaagag ttgctggata aaatgctggc ggcgaagaac taccaggcgg
     7441 cgctgtttat tctgcgccag ctggagttcg gcctgttcga tttccgcctc catgccgagt
     7501 tccgcccgga tcagggagcg aaaatcctcg aaactctggc agaaatcaag aaactggttg
     7561 ccgtagtacc gtctccatcc tggggccgtt tcccgcacgc tttcagccat attttcgccg
     7621 gtggttatgc cgcaggttac tacagctacc tgtgggctga cgtactggcg gcagatgctt
     7681 tctcgcgctt tgaggaagag ggcattttca accgtgaaac cgggcagtcg ttcctcgaca
     7741 acattctgag ccgtggcggt tcagaagagc cgatggatct gttcaaacgc ttccgtggtc
     7801 gtgaaccgca gctggatgcg atgctggagc attacggcat taagggctga tcattcagtg
     7861 aaaatctgct taattgatga aacaggcacc ggagacggtg ccttatctgt tctggcggcc
     7921 cgctgggggc tggagcacga tgaagacaac ctgatggcgc tggtgttaac gccggaacat
     7981 ctggaattgc gcaagcgtga tgagccaaaa cttggcggca tctttgttga ttttgttggc
     8041 ggagcgatgg cgcaccgacg caaattcggc ggtggtcgcg gtgaggcggt ggcgaaagcg
     8101 gtgggcatta aaggcgatta tttgccggat gtggtggacg ctaccgcagg gctggggcgc
     8161 gatgcctttg tgctggcttc ggtcggctgc cgcgtgcgga tgctggagcg taatccagtc
     8221 gttgccgcgc tactcgacga cggcctggcg cgtggatatg cggatgcgga aatcggcggc
     8281 tggttgcagg agcgtttgca gttaattcac gcctccagcc tgacggcgct gaccgatatt
     8341 accccgcgtc cgcaggtggt ctatctcgac ccgatgttcc cgcataagca gaaaagcgcg
     8401 ctggtgaaaa aagagatgcg cgtctttcag tcgctggtgg gaccggatct tgatgccgat
     8461 ggattactgg agcctgcacg cttgctggcg accaaacgcg tggtggtaaa gcgcccggac
     8521 tacgcgccac cgctagcgaa tgtcgccacg ccaaacgcgg ttgtgactaa agggcatcgc
     8581 tttgatatct atgcaggtac gccagtgtaa aagtaaagca gggtagcgat aacgctaccc
     8641 tgctttctgc aagaataatt aatggctttc cggcgtcgca atcatgcgtt tcagccacgg
     8701 caccatcaac agcatcacta ctgcaacgcc cagcgtgacc agaccaatct taccaaacac
     8761 gttggtatag acaggcaacg tctcaagcgg atcggtaatg ttgtctggta ctgcggtaaa
     8821 tgttgccaca tagccgccca gcaagaacgc ggcagcctgt gtcaggaacc acatcccgag
     8881 aataaagccc atcaaatgct gcggcaccag agcagcaatc atcgccaggc caagggcgct
     8941 aataaacagt tcacctaagc tctggaataa gtacaccagc acgataaacc atggcgatgt
     9001 cagcccttgt gcatccgcaa accacattcc cgcagctgcc gccgtcaaaa agcccagtga
     9061 gcacataaac atgccgagag taaatttcat cggcatcgag aggtctttgc ctttgttacc
     9121 cagatgcgtg taaatgcctg ccagtattgg gctggcgagt accacccaga acgggttaag
     9181 cgcctggaag ctgaccgggt tgatggaaaa accgagaatt tcatgatgca cgttgttgat
     9241 ggcaaagaag ttcagcgatg ttggcatctg ggcgtagaga atgtaaaaca ccaccgcttc
     9301 gagcatcagg acaaaggcga caaacatttt attgcgcccg gttttatcca gcttgaatgc
     9361 ctgacgaaag aagatgatgg tgacgacgat ggagagaaca atcagcacca gattggcgac
     9421 ttctacgttg tgcatcagcc aggcgcagac gaagatcatc accacgctgc caagtaatac
     9481 gtacaacagt ttgctgaagc tcattggccg gaagtcgggc tcagaaccaa tgtctttcac
     9541 cattccacga caggcgatgt aaaccagtaa tgcgataatt aaccccgcac cgcacaggtt
     9601 gtaggtgact gaatagccga atttatcagc gatcacaggg gccagcgata acgctatcaa
     9661 cgagccgata ttgatcgaca tatagaacag ggtgaatgtg ccatcaaggc gcggatcttt
     9721 cggcggatag cacttcgaaa gcaagctggc tgggttagct ttaaacaggc cgttaccgac
     9781 agcgatagtc cccagggcga tgaaaatcag gtcaggctta agtagcgaca tgccggtcat
     9841 gaagtagcca atcgccagca caagtgctcc aagaacgatg gtgcgtttgg tccccagcag
     9901 gtggtcgccg acatagccgc caatggaaat gaggccatag accagtgcag caaaagcacc
     9961 aaaagtgaca aaagcctgct cttgcgagaa tccaagctgt ttaacgaaga aaaccgccag
    10021 tacgccctgc acgccgtagt agccgaatcg ctcccataac tcgacaaaaa agatcatgaa
    10081 aaatgggcga ggttgctgca gcatccccat gggtgttgtt gtattcatat ttattaacct
    10141 tccaatacca tcctgaaaag tatgaacgcg taagggataa cgcgcgaaaa agtttacatc
    10201 tgtcagagat tcttaatatt gatgcggtga atagattaat acactattac aacagaaaat
    10261 aaccagctga tcacactaat gatggaaaat atgatggtgt taaatattgt attggctggg
    10321 gattttgatt gcgaacgcag accgtaggcc agataaggcg tttacgctga tcaggtaaca
    10381 aggcgcgaca aaaagcaaaa cgcccgcata tgcgggcgtt caggaacacg tcgtggaggg
    10441 aagattattc ttcttcgtcg cgcagcggaa caatcagcat atcaacgtga acggtgttga
    10501 tcagctgacg tgcggaagac atcagtttgc tccagaagtc ctggtggtga ccacaaacca
    10561 ctagatccat atcgtatttc ttgattgcat cgaccagaac ctggcccagg tcgccgctgc
    10621 cgctcagggt ttcagtgatt gggtagcctg cattagtgga aagctcggtc agcgcatgat
    10681 gtgtctcttc agagatgcgt ttctgcatat caccaagatt cacatcaata agcccggtat
    10741 ataggtcaga gtagtttaca tctacgtgga tcagagaaac tttcgcattg tatgggcgag
    10801 ccatagagac tgctttctct accagaactt tgctttccgg ggagagatcg accgcgatga
    10861 gaatgtgttt ataagccata gtgttactcc ttccataaag ttgtcgatga ctggccagct
    10921 agcgtttctt gtgccgttac gtcacccgcg tcctgcgaac tacgtgcgcg gggctaaccc
    10981 agccgtaaag attcagtgtt aagaccatcc ttaccttata gcgacccgga tgatccgtca
    11041 atccgccttg cttacccgtt agataaacaa attttcgaca tgaatatatt gatagtggtt
    11101 aaccttctgg aaaaaaaaca acctgatctc ctacactatc tatagagccg ctcggatgtt
    11161 ggtcaccaca aaagcggttt ctccaggacc tggtgttaac tgattggcag cgtatcggga
    11221 gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta tgataagcac
    11281 cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc gctatttctc
    11341 atcactacgc gcgttgttag tggtactgcg taactgcgat ccattgctct atcaatatgt
    11401 tgatggaggg ggctttttta cctcacatgg ccaacccaac aaacaggtgc gtctcgtttg
    11461 gtatatctat gcccaacgtt atcgcgatca tcacgatgat gagtttattc gccgctgtga
    11521 gcgggtgcgt cggcagttta ttctgaccag cgcattgtgt ggtctggtgg tggtcagcct
    11581 gattgccttg atgatttggc attaagcact tcagcaataa taacgcataa aaaaagcggg
    11641 ccagtaaact gacccgctcg ttttggtgcg tactattaca ggaactgcaa ggagagccag
    11701 taaagcccgc cggaaagcag taccgcagcc ggaagggtaa acacccaggc catcagaatg
    11761 ctggtcacgg ttttacgctg taagccgcca ccatctacca ccatcgtccc cgcgacagaa
    11821 gaggagagta cgtgagtagt ggaaaccggc atcccggtat aactcgccag gccgatagac
    11881 actgccgcag tcatctgggc agacatcccc tgagcgtagg tcatgccttt cttaccgatt
    11941 ttctcaccga tagtcgttgc cacacgacgc cagccaatca tcgtaccgat acctaacgcc
    12001 agcgcgaccg ccatgatgat ccacaccggt gcatactcga tggtgctaag catgtcggac
    12061 ttcagtttct tcaacaggcg ctgatcgtca gcactcacgc caggcatctt caccactttg
    12121 tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg atcaagcgac
    12181 agcttgtcgt agctttccac atcggtggtc aacatacctt tcaggcggtt gagcgcgtta
    12241 atggtattcg acggatggca gtggaactcc gcaggttgcg ttgcgccagc ttccggagcc
    12301 ggtactaact gatcagcacc agtagcttgt ttcagcagcg caggatgctg ctcaaagtaa
    12361 gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt ggcattcatg
    12421 ttcaccacga agcctgctgg tgcgacacca atcaatacca acataaccag accaatgcct
    12481 ttctgaccat cgtttgcgcc gtgcgaaaac gccacgccga tagcggaaag gatcagcgcg
    12541 atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc cgctggggtc
    12601 aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa gaaaatcaga
    12661 ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa aatacttaat
    12721 actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc attggttaaa
    12781 ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggcaa accaaagtac
    12841 caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat ggcaaggcca
    12901 tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat ggcataggca
    12961 acactcagac cacccagcaa aacacccaaa aagttgaaca ccgctgccat aaccacggcg
    13021 agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc tgtgtcatgg
    13081 aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa taacagcccg
    13141 gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat tttgaggaca
    13201 tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata tagacttttt
    13261 ttgatttgcc tcctgtatgg atttcactca aaaaataatt atcttatata attcaggcaa
    13321 atacttcctt ttagtaatat tgatgctggt gcgaccactg aggaatcttt acaattcacg
    13381 cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga tgccattatt ataggcgctg
    13441 gtgcggcggg tatgttctgt tctgcgctgg caggtcaggc aggacgccgg gttctgctga
    13501 tcgataatgg taaaaaacca gggcgcaaaa tccttatgtc tggcggtggg cgctgcaact
    13561 ttaccaacct ttatgtcgaa ccgggcgctt atctgagcca gaatccgcat ttttgtaagt
    13621 ctgcactcgc gcgttttacc cagtgggatt tcattgatct ggtcaataaa cacggcatcg
    13681 cctggcacga gaaaacgtta gggcaactct tctgcgatga ctccgcgcag cagattgtcg
    13741 acatgctggt ggatgagtgc gagaagggca atgtgaccgt cagattgcgt agcgaagtgc
    13801 tgagtgtggc gaaggatgat acaggcttca cgcttgaact gaacggcatg actgtcggtt
    13861 gtgaaaagct ggtcatcgcg accggcgggc tgtcaatgcc gggcctgggc gcgtcgccgt
    13921 ttggttataa gattgccgaa caatttggcc tcaacgtgct gccgacccgc gcgggtctgg
    13981 tgccattcac tctgcataaa ccgttgctcg aagagttaca ggtgctggcg ggcgtggcgg
    14041 tgccttccgt gattaccgct gaaaacggca ccgttttccg tgagaactta ctcttcaccc
    14101 atcgcggctt gtctggaccg gcggtgttgc aaatttccag ctactggcaa ccgggggaat
    14161 ttgtcagtat caatctgcta ccggatgtgg acctcgaaac cttcctgaat gagcagcgta
    14221 acgcacatcc gaatcagagc ctgaaaaaca cactggcggt tcatctaccg aagcggttgg
    14281 ttgaacgctt acagcaactc gggcaaatcc cggatgtttc gctaaaacag ctcaacgtgc
    14341 gtgaccaaca ggcactgatt agcacattga ccgactggcg cgtacaaccc aacggcactg
    14401 aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa ctctcttcac
    14461 ggacgatgga agcgcgcaaa gtgcctgggc tgtactttat cggcgaagtg atggacgtta
    14521 ccggctggct ggggggctat aacttccagt gggcgtggtc gagtgcgtgg gcttgtgcgc
    14581 aggatttgat tgcagcgaag tcgtgctgag ctatttttcc taaagatgaa aattattaga
    14641 gtcgcattaa aaatgagcta attttgatag tggctatctt gtgattattt tctaatgagc
    14701 ccgtgaactg aaccccccat gcttaatata aggtggaggg aaaggtgatt gaaaactcac
    14761 ccagtgggct gatctttata aaatatgaac aggttgaaaa aaaaacagta actttgctgt
    14821 tttttatact taaccactat gcattaatgc tgcgttattt cattctacct aagaaaaacc
    14881 agcgttacgc aaatggttaa cgctggtttt atccggtacg ttgcaattat tttttagcag
    14941 aacccgcttc taatattgaa acgattgaga acaacgtaaa gcataccgct cccaaaccga
    15001 ccagaacccg cgaaggaata aaatatccca tatcggtctg cgccatttcc gcaagaaacg
    15061 atgctaggaa aaggcaaaac aggcaggtga agacgggaat catcggtatt ctgttggcta
    15121 acgaacaggt acgtctccag accagtgcca gtagccagac ttttgagaat atgctgtaac
    15181 agatcattcc caagcaaatg aggataatac cgggagccag tcgggcgctt gcatcagaac
    15241 tgactaagac gtatatgccc tgcagcaccg tgattgagcc gagaaaaata acccaataac
    15301 accacagcca gtgctctttc gttgaaaacg tattacgtgt ttgatggacg attgtggcaa
    15361 caaggccaat tagacaggca cagattgcgg ttaacccgag caatacgtga ccggcgacat
    15421 aatgcggagt tatgtcggca ctacgtaaca gcgtaataga ccaaatgaaa ccgagcaggg
    15481 tgagtaacac gggaacggca atgaggcaat taccgattaa tgaagaataa gcctgtaccg
    15541 gtgttccgtc gctcttgctc cccgctgcgt ttttaggaat gagcagaaag tgaccggatg
    15601 acgctgccac cgtcgataca caggcggcaa tcataccaac gccgaaaata acatggccgg
    15661 cgacaaactc gtctgccatc acatcattac ctgctaacag tgcccaaccc catatcatgg
    15721 tgataattga ccccgcatag ccaataatgg ggaacaatgt attgtaaaac gtattaacgc
    15781 cgcgcgtgag ctgcgaaatg ataataaatg cggtagtgaa taatgccagg catatggctg
    15841 ccagagaaat gagcacatga cccgcgacga aatactcgct ttgccctgaa ctttccagca
    15901 caaagccacc taacgcgata caaatcaagc ccataattaa agggattaat ttgaaaagcc
    15961 acccgatata tatgttcact ttaaactcct caggtatccc tgctaatatt gaaatgtgca
    16021 ttaatgataa tttcagcctg ttaatacaat gacgagatat cattaaagac aaatgcatag
    16081 cataactata gcggggttta ctttgcagag cgctactttt cagcgttcaa aaggccatga
    16141 taaataaata tttatcctaa gattaattct tattctgttg ttttttacaa ttatcttcca
    16201 gggagggatt ctttttcatt atttggtaaa agaccggatg gtctcggtgc ttaaaatcag
    16261 cgagtatata cgcatcattg ctttatatcg tcaaaaaata tgtctttcag acatgtatca
    16321 tttattttgt tactaacaag tcaaaaatat cacgggttat ttatgaaagt atttcacaat
    16381 atatttaagt acatctcttc aaaccatcaa gataagcatt cagataaagt taataatcat
    16441 caacaccatg gcaaggttga taaaaaacat cgttcagaga tagttgaaat tgataagctg
    16501 gataaacatt cgcaaataga taacgatttt ggcctgcata tcatctattt cctgcaacat
    16561 ggctgctgga acgtaactga acatagtcct cagatagaga aaatatggtt ttacaatag
//
LOCUS       DABSYT020000099        16064 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.099, whole genome shotgun sequence.
ACCESSION   DABSYT020000099 DABSYT020000000
VERSION     DABSYT020000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.099"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..368)
                     /locus_tag="ID725_RS18995"
                     /old_locus_tag="pgaptmp_003799"
     CDS             complement(<1..368)
                     /locus_tag="ID725_RS18995"
                     /old_locus_tag="pgaptmp_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGV7852300.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
     gene            complement(378..707)
                     /locus_tag="ID725_RS19000"
                     /old_locus_tag="pgaptmp_003800"
     CDS             complement(378..707)
                     /locus_tag="ID725_RS19000"
                     /old_locus_tag="pgaptmp_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7852301.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(707..3772)
                     /locus_tag="ID725_RS19005"
                     /old_locus_tag="pgaptmp_003801"
     CDS             complement(707..3772)
                     /locus_tag="ID725_RS19005"
                     /old_locus_tag="pgaptmp_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGV7852302.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            complement(3744..4073)
                     /locus_tag="ID725_RS19010"
                     /old_locus_tag="pgaptmp_003802"
     CDS             complement(3744..4073)
                     /locus_tag="ID725_RS19010"
                     /old_locus_tag="pgaptmp_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGV7852303.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(4082..4468)
                     /locus_tag="ID725_RS19015"
                     /old_locus_tag="pgaptmp_003803"
     CDS             complement(4082..4468)
                     /locus_tag="ID725_RS19015"
                     /old_locus_tag="pgaptmp_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGV7852304.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            complement(4529..5272)
                     /locus_tag="ID725_RS19020"
                     /old_locus_tag="pgaptmp_003804"
     CDS             complement(4529..5272)
                     /locus_tag="ID725_RS19020"
                     /old_locus_tag="pgaptmp_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7852305.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            complement(5283..5684)
                     /locus_tag="ID725_RS19025"
                     /old_locus_tag="pgaptmp_003805"
     CDS             complement(5283..5684)
                     /locus_tag="ID725_RS19025"
                     /old_locus_tag="pgaptmp_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGV7852306.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVSALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(5681..6259)
                     /locus_tag="ID725_RS19030"
                     /old_locus_tag="pgaptmp_003806"
     CDS             complement(5681..6259)
                     /locus_tag="ID725_RS19030"
                     /old_locus_tag="pgaptmp_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7852307.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(6271..6546)
                     /locus_tag="ID725_RS19035"
                     /old_locus_tag="pgaptmp_003807"
     CDS             complement(6271..6546)
                     /locus_tag="ID725_RS19035"
                     /old_locus_tag="pgaptmp_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGV7852308.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(6539..6862)
                     /locus_tag="ID725_RS19040"
                     /old_locus_tag="pgaptmp_003808"
     CDS             complement(6539..6862)
                     /locus_tag="ID725_RS19040"
                     /old_locus_tag="pgaptmp_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HGV7852309.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(6949..8976)
                     /locus_tag="ID725_RS19045"
                     /old_locus_tag="pgaptmp_003809"
     CDS             complement(6949..8976)
                     /locus_tag="ID725_RS19045"
                     /old_locus_tag="pgaptmp_003809"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGV7852310.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(8921..9256)
                     /locus_tag="ID725_RS19050"
                     /old_locus_tag="pgaptmp_003810"
     CDS             complement(8921..9256)
                     /locus_tag="ID725_RS19050"
                     /old_locus_tag="pgaptmp_003810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGV7852311.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            complement(9378..10502)
                     /locus_tag="ID725_RS19055"
                     /old_locus_tag="pgaptmp_003811"
     CDS             complement(9378..10502)
                     /locus_tag="ID725_RS19055"
                     /old_locus_tag="pgaptmp_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGV7852312.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            complement(10430..10642)
                     /locus_tag="ID725_RS19060"
                     /old_locus_tag="pgaptmp_003812"
     CDS             complement(10430..10642)
                     /locus_tag="ID725_RS19060"
                     /old_locus_tag="pgaptmp_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852313.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(10639..12741)
                     /locus_tag="ID725_RS19065"
                     /old_locus_tag="pgaptmp_003813"
     CDS             complement(10639..12741)
                     /locus_tag="ID725_RS19065"
                     /old_locus_tag="pgaptmp_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGV7852314.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            complement(12741..13232)
                     /locus_tag="ID725_RS19070"
                     /old_locus_tag="pgaptmp_003814"
     CDS             complement(12741..13232)
                     /locus_tag="ID725_RS19070"
                     /old_locus_tag="pgaptmp_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGV7852315.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            complement(13907..14059)
                     /locus_tag="ID725_RS19075"
                     /old_locus_tag="pgaptmp_003815"
     CDS             complement(13907..14059)
                     /locus_tag="ID725_RS19075"
                     /old_locus_tag="pgaptmp_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852316.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(14047..14514)
                     /locus_tag="ID725_RS19080"
                     /old_locus_tag="pgaptmp_003816"
     CDS             complement(14047..14514)
                     /locus_tag="ID725_RS19080"
                     /old_locus_tag="pgaptmp_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGV7852317.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(14511..15008)
                     /gene="rrrD"
                     /locus_tag="ID725_RS19085"
                     /old_locus_tag="pgaptmp_003817"
     CDS             complement(14511..15008)
                     /gene="rrrD"
                     /locus_tag="ID725_RS19085"
                     /old_locus_tag="pgaptmp_003817"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGV7852318.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(15008..15223)
                     /locus_tag="ID725_RS19090"
                     /old_locus_tag="pgaptmp_003818"
     CDS             complement(15008..15223)
                     /locus_tag="ID725_RS19090"
                     /old_locus_tag="pgaptmp_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGV7852319.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVSGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            15366..15764
                     /locus_tag="ID725_RS19095"
                     /old_locus_tag="pgaptmp_003819"
     CDS             15366..15764
                     /locus_tag="ID725_RS19095"
                     /old_locus_tag="pgaptmp_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852320.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(15845..16003)
                     /locus_tag="ID725_RS19100"
                     /old_locus_tag="pgaptmp_003820"
     CDS             complement(15845..16003)
                     /locus_tag="ID725_RS19100"
                     /old_locus_tag="pgaptmp_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGV7852321.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
BASE COUNT         3676 a         4771 c         3686 g         3931 t
ORIGIN      
        1 ctgtttccgt tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgccggacc
       61 accgttccgc cgaccagact ctgcaggtct tccgccatcc ccgtgaccat gccgtgcagg
      121 ttagaaaccg tcagtgtcgg acgggcagaa ctgcccttgc cgttcagttc aaatcccgtc
      181 ccctgaatgg ggtatgcctg atactgccgc ccctgccagg taaccggctc acctttttcg
      241 ttctgctcat tacagaaaaa ataacgttca ccaccgacct ctgtcagatc gatttcccag
      301 agcaccgcct gggctgactg agtgaggcgt gtcgtctcat gatgtgtttc ctgtggaata
      361 tcctgcatca gagcctccta tgccacgacc tgttcaaaat ctgccgttat ggttacccac
      421 agcgccccca cgcttgccga ccatttacga caaaccaccc tgatcggttt ccagtcataa
      481 ggtggcgtcc actgaaatgc acggacgcca ccgtgccgtt ccagaaaggc ttttaaagat
      541 gggtgttcac atttacgaac acgtatcgtc acgctgtaag tcgacaactg gttattcagt
      601 cccgccgcac gacgctgttc ataaccatcg cccagcttca ctgtcaccac tttcggctct
      661 gataccacat tcatatccgg gcgcactttc cagtgaaacg tctccattac cgatatgctc
      721 cacttaaccg accaccatca cgggcctgct gttgcataaa gtccgctgcc gcttttttcc
      781 cgaggtcata aaccgccttc agggcagccg gacctatctg cccgttcgtg ccatcgttat
      841 tgatctcgat gttgtactgc ggggaaaaca tcgccatacc tgaaccccca atatccgcca
      901 caacccccag cttaccgtca gcaccacgac gcaatggcag aatggcttca ggtcccgctt
      961 cccccatcac acccgcgcct tttgcaaaag caaaaaacgt cggacggtta accaccgtgc
     1021 cactgtaacg actcaaatca gcagactgat aaatcccccc attggcattc agggaaaggc
     1081 tgctgagatc aaatcccagt acactgccaa tcccttttat cgatttcatc atggttgcct
     1141 gcgccaggat tttcgccata tctgacagca cagaagaggt gaaggatttg aaattgagtt
     1201 tgccggtagt gacaaaagtt gccaggccat ttcccatgtt gctgaaggct gacgagaaca
     1261 cctgttctgc tgtttctgcc gtattatctg catccacggt aaaatcctga aatgcacgca
     1321 ggactccgtt tttccagtta ccctgtacaa cttcaagctg ttgccagtaa cggcgattct
     1381 cattcagttg tcggttcagg ctctccgtca gcgcctgctc ggcctttctg tagtcatccg
     1441 tgttatatgt ccctttctgc tcactatccc gcctcaactg ctccagctgt tgctggtatt
     1501 tctggcgaag actcagttgt acctgatatc gctgtcgctg ctgatcaccc atacccaccg
     1561 tggcgatatc caggtcatgt tgctgacgct gagcgcgctc ttcttcagcc agttgactgg
     1621 tcagctgaat tgtttttttc ttcagctcgt tgagtgccgt ctgtttctga agctcctgct
     1681 gttttacatc cagcagcgtc agtgcctgaa tcagttcatc tttacgggcc agcacactct
     1741 tttcatctgc cgtcagtttt ttcccgtcca ggtcgctgat gcgctgctgc agagccagaa
     1801 gctgtttacg cgcttctgtc atcctttccg tggcaatgcc tgctgactgt ctggcagcag
     1861 caatctgtcc ttccacctgt gcctgttgct gactgtactg cagcaataac cgggtggcct
     1921 catcattacg ggtttcgcgt gtttttttct taccggatgc cagggctttc tcgtaacgtt
     1981 cattttcacg ttgtatcgcc gcatccctga cagcctgatc ggcgtactgc atggcattaa
     2041 tacgcgctat ttcacgctga tgtcgtgctg cttccgtttc attcatccgg ttcagtgcag
     2101 cattttcagc attacggcgt ttctgttgct cctgataatt ccgctctgcc tgctcttttg
     2161 catcctgcaa atccttctgg cgttttttct cctgaagatc gttaagacgc tgctgatcgt
     2221 attcaacctg agaagatgat gccgtccagg ggagtctttt cgcccgcgac actttctcct
     2281 gtaaagcggc aatctgttca tccagcgagt cttcacgacc aatattcatg gccgcatccc
     2341 agaaacgact ccacaaatca gacagatact tcagcgtact gccaagcgca ttgaggttat
     2401 tatcaatatc cgcagtacgc cgaccggttt cctctgccag tgcagacatg gctatccgtg
     2461 cagcatcact ggaccgcccc tgctccccaa ggacgcgtat ctgctcaagc tgagtggcag
     2521 taagaaaatg cagctcattg tccagagcct tcgcggcatt tacaggatca tccttcagcc
     2581 gcttaaactg atttatggta tcgctgaccg actggccaac cgatcgctcc atctgtgcgg
     2641 cagctctcgc caccataccg atatcgtttc cacgaaatgc accactcccc accacctgag
     2701 ccagcgcacc ggctgcagca tgttgcgtga taccattccc ggaaatagca cgactgagcg
     2761 tccacagctg cccggcagtg actccggcat aatgccccgt cagcgacagc tggcggttaa
     2821 attcttcccc ctccttctga ccgtcatacc aggctttacc cagaccatag acagccgcgg
     2881 caataccgcc aataaccgct cctggcatca tgcctttcgg tgacatcagt gtgtctatcc
     2941 acccggcacg gttagccagc gttattccgg atcccctcag cgcacctaaa ttgccgcggg
     3001 ccagttcacc tatcagaacg cctatctcct gacgggctgc tgcacttttc agacccagcg
     3061 aatgcgtggc ttttcctgcc tgctccattt tgcggatata cacttctgca gcactgctta
     3121 cccccagctg ggcagcctta gcacgaagca actcagaaga agaaagattc tggcgggttg
     3181 cctgctcttt aagctgacgg ataaacgcca ctttctgtcg ggtagcctct tcctcagcct
     3241 gtgtaagaac acgggttttc gccgtaacct cagaaatcag cgccagataa tcctgctgac
     3301 caatcccgcc actgtttctg gcctgtcgga tctgttgctg aatacgctgt aattcctgca
     3361 gccccgcacc ggcctgtttc acactgtcaa tctgacgata aaacgcagca gccgctttat
     3421 cctgagcctc cgccagagcc atggcctgcg cctgttcctc gcgcattttc tggctcagtg
     3481 cctccatacg ctggcgggtt tgctccacct cgcgggccat gcgttcatga gcctgtgcgc
     3541 tcttctccac cgtctgcgca tggacggatg cggctgttgc agccgaagaa gccgcctgca
     3601 ttgtctgccg ggcggcctga gtctgacgct ccataaaacg ctgcatacgg gcagaagacc
     3661 gttctgcatc gctggctgca ccattcagaa ggtttttgat acggggaatt tcatttttaa
     3721 actctgccgc atcaatcccc aaatcaatga ccaggttggc tatctggtcc ataacgcaca
     3781 cctccggaaa taccttcccc aagatgcatc agttcttcgt ccgttcgctc cggtatcccg
     3841 ttctcttccg gtaaaaggct gaaatcagcc accgcagcat cactgctacc ggacaccatt
     3901 ctcacgatca atgccttcag cgaggcaaac tgcgcatcca tccacacatc actgaagctc
     3961 tgcatccgga aataatcgcc ccactcacca agctcagtgg ccgacatttc cgacagcatc
     4021 cgccgccagt ctgcccgccg gaactcccgg gcaagccgca tgacaaactg catttcccgc
     4081 gtcaggactt ttccggcgtc agcacctcat gctccaaatc cccggcattc tcaatggctc
     4141 ccataccgct cagcgacaga accatctccg cccccgctcc cagggcatca tacgaccatg
     4201 ttgtaataac ggatgcgcaa agcgtctcaa catcctgaga ctgttccgca ttccacagtg
     4261 agcgggaaac cagccaggca ttgatatcca tccccatccg cagaaaagca atctgtcgtt
     4321 cagcctccgg cagttctccc tcttcggcat caaactttgc cgttcgctgc tgaacaaacg
     4381 ccagatattc aattctctgc agcccggaca gctcactgag caccacggac tgcttttcat
     4441 aattaaacgt gccctgtttc agaaacatca tgttctccac ctgcaaaaaa gccccggata
     4501 accggggcaa atgatgagta tcgtcctgtt aacctgcagc gctgacagtc accgcagcca
     4561 cagccacaaa attcccgtca gcggtcatgc ccacaatgct gacgctgccc tgcttcacgc
     4621 ctttcaccgt ggccacaagt ccgttcaggg tcacagtggc tgtctgtgga tctgtcgaat
     4681 gcacactgat cgctttgtca ctggctccgt caggttttac tgtaaaggtc agcgtggtgg
     4741 ttgctcccac attgacactg gcggatgccg gtgccaccgt cagcccggta acgcccacag
     4801 tttcagtgcc ctcctctgcc agatatggac gccccacacc gctgattttc actgtgcggg
     4861 tcatcacgtc ttttgaggca atggttttac ccagtgagct cagccagccg cggaaaacat
     4921 caacagtgcc gttgggatat ttgatacgaa acgcgcagac ttcaccggag tcgaacaact
     4981 gaaccagttt tttctgcccg ctgtcacccg gacgccaggc cagcgtcgcc gaagtatcac
     5041 caacggattt ctgcccctgg gttgtcgttt tccagtctgc atcttcatca tcgagataag
     5101 tgtcatcttc tgcatcagcg gtcatttcgc caggttgcag atccttcacc atcgcaagac
     5161 gcagccagtc agtgtccgac aaagggttcg caaacgcatc gccgttgccg gtatacatcc
     5221 agaacgtcgt ccccgcacct ttcgtctttg ccagtggatt tggcgtggtc attaccacct
     5281 ccttaattcg tgtacgtgat ctggtacgtg atttccgcca tcgcccaggt ggccatctca
     5341 ttatcacgtt gatagttaaa accgagtggg atcagggtgt cgatgagtcc ggaaagtgcc
     5401 gatacatcat tcagggccgg gaaaatggtg ctctccatcc acatatccag ctctgaatcc
     5461 ggtgcctgtg cccggatgaa gacagcaata tgcagaacag cctgccagtc atcttcatcc
     5521 gtcatttttc cggtgtactg agcatcactc agccacaccg ccacggcagg cagttcctgc
     5581 gcatcaacaa atgccggaag cccgtcaaaa aacgtggcgc tgtctccaca ctgttcccga
     5641 aggcgtgcca gtacgacctg gcggatttgt gtatgtcggt tcatcgggtc agccataacc
     5701 tcagttgttg tttcagtgca taccccagct gtttcggcat ttccgcagca atgatgcggt
     5761 cgcgggcatc ttcaaatgcc tgtgtcagcg gtccggacag cgggattttc accacatcaa
     5821 tggggtaacg atttttgcca tcaatacgcc gcatcacatg ccagcgacca ttcgccagtt
     5881 gctgaataaa cgcatcccgg aaaagatatt tacccacctt cagcacactg ccacggtact
     5941 gcagttttcc accacgctgg gccagtctga cccgggctgt ccccagctta atggcgggca
     6001 gattgccccg gttaacgcgg atcctggccg tcatttttcc tgacggactg gctttaaaca
     6061 cccggacacg ctgacgaacc agtttcaggg ggatcccttt cacctggtta tctcccgcaa
     6121 cggtattccc ggcaacctgc cgggtggcaa ccgagcccgc tttctgtgcc acacggttta
     6181 tcgcccatgc gctggcctgt ggcaccatac gggtatcaag gctgttcaga ttgcggatgg
     6241 cattctcaag ccccttcatc ccacacctct ttactcaata aagatcattg gcttaccgtt
     6301 aaagcgttca tgccgtgtga ccgtccattg ttgtccgtca taaacaacgc gatccccgcg
     6361 ccgtgggcgg tatcccgaag aaaacaccac cagagagacc gcaggtccgg acagagcatt
     6421 cagctctgcc agtgtttctc ccgggatcac agtcatatcg acatcattaa tcgaggctgt
     6481 ctttcccatc tttctgaccg tgatagcatc catacgcgct gccagccggg aaaagggatc
     6541 agacattgag ttttaccggc acttcttctg cactggttcc ggcatctgcc cagacaaccc
     6601 cgaccagcgg atcagagccg ctgttagtca gctgaacttt tcctgacttc agataaacct
     6661 tcttacccgt tttcatgtca tccgttttca gcttaggcag cataaacaca ccttcggtca
     6721 tgccgtcgcc tgtttcaccc tgtggaatat cggtcagcgc caccgcaaaa acatcgccca
     6781 cctgcaccag atctccgctg ctgatggctg cactggcaac aatcgccacc gtttttcctt
     6841 cttctacaaa attctttgcc ataactgtct ccgcacagcc ccgttcaggg gctgatttca
     6901 ggtacaaaaa aagcccttac gggccatcag agttgttgtc tgcgacgttt acgccgtaca
     6961 tttcaccaga ccgcggtgat caactggcgc gacaccggcg tcaatacgca ctttcgttgt
     7021 cacgccatcc acactgaagc cctccatctg atcaatatat ggcgtatcca caccgttgag
     7081 ataagccact tcaatcgtat cggagccttt tgacgcagcc aggtagaagg tggtctggct
     7141 gttatcatca agacgaggct ctgcaataac ggtcgcaaaa tctttcaccg ggttaataat
     7201 accggcgtta atgtcagccc ccttgacact tgaggagcga atgacctggt tagcaacaga
     7261 ctccatcgcc gtcggtacca gtacgaacgc aggacgaata ttcagatgac gctccccctc
     7321 tttctgaacg cgcatcaact ggcgggcttt atccagcgat gccacgtcca ttgcagcgct
     7381 ctccagtacg tttgcatgtt tcgctttatc gaacagactt acattatctg tggagatttt
     7441 cgggttagac gtcagaatgg cataaaccag atcggcaata gtggatttcg ccgcacggcc
     7501 cagtttcatc gggacatcgg tcagcatatt cagatcatca ttgataatgg cctgacgggt
     7561 gatactgaac agctcgccat aggtcgccag tgcaatagtg gcctgtttat ctccggtggt
     7621 gacgtattta tattccgccc cttcacgcac ctgacgcaga gcactgaagc cccccatacc
     7681 cacacgatgg gcaattttaa aatcagacaa ctgacctttc cgcgtccact gttcataggt
     7741 ttcaggggca tcttcccagc cctgcagaat ggctttgttc gcaacatcca gcagaatatt
     7801 accgaagtca gacgtactgt gtgtgaacgc cgcaccgacc atctgcatcg ggttataact
     7861 ggaaacccca ataccccgtt cagtcagtga catacgggca tattcacgca gggtcatccc
     7921 gttgtagaca ttatcacgtt cggttttttc aaatccggca cgcgccatca gcgcctggcg
     7981 gatcccatcc cccacaaaat taccgtttcc ggcataaata tgagccgggg tatttttatt
     8041 agatggcgtg gactcgcgcc ccatctcgtt caacagcttt tcgcgggcct gctccagcga
     8101 acattcagga tcggcaagac actgagcctg cagcgtctga taacgcccgc caaacatggc
     8161 aaacagatca ttaataccgt ttacacgcgc tttttgctct gccagtacct gcgcacggat
     8221 actgttttca tccaccacag gtgctgctgc ctgcactggc gtccgggagg ctgcaggtcc
     8281 atcatcctgt acgcgtggag cactgttgcg tggcggagta atcatgtttc gaatggattc
     8341 cggcatcttt ttaaattcct ctgtacgttt tgactgaata catgccattg ccttaacggc
     8401 tggcgtcacc tgatcagcaa atccatgtgc cagacattcg gcaccggaca tccaggtctc
     8461 atccgccagc atggcagcaa tttcatcggt ggttttcccg gttttctgtg cataagcggg
     8521 taacagaacc gcctcaactt tatcgagcag gtcggcatag gtgcgcatgt cctccgcatc
     8581 accgcccgta aagccaaatg gtttatgaat catcatgaag gagttttccg gcataatgac
     8641 cggttttccc accatcgcaa tgaccgacgc cattgacgcc gccacaccgt cgacataaac
     8701 ggtaatggac gcaccatgtg ttttcagtgc attaaaaatg gcgatgcctt caaagacatc
     8761 gccacccggt gaattgatat tgagattaat gtgggtgata tcgcccagtg cattcagatc
     8821 actgataaac tgcttcgctg taacacccca gaaaccaatc tcgtcataaa tataaatatc
     8881 cgcgtcactc tggtgaccag cctgcatcct gaaccaggaa ttattcttcg gactggtcgt
     8941 cggtgtgctg cggctcctgt cgtttcgttg cggcactgct gcctccttta tcactggccg
     9001 gatcggtatc aaataccaga tccagcttgc ggttttcatc aatttcggcc ttgcgccgac
     9061 gtttgacatc atccggatta cgaccacctg cacgtaccca gtctgattct gtcgctgctc
     9121 caccacgaat ctgaattttc caggcctcag cctccttaac agggtcaatc cacggcatca
     9181 ccggtccgga atacaccgcg gtatacagtg aagaacggtc aagatcgcgg ggtagcctga
     9241 taacaccgga tgccacagcc tgtttcagcc aggcacgata catcgggcgg gtgacggcac
     9301 caataaacca gtcctgcagg atcaggtagc catcagtgga ttcaaccagc tcctgacgct
     9361 gggcgctgta agtgccgtta tagttgcgcg ctgtactgga aaaaattcag acgactgccc
     9421 gccgccacgg cacgcaactg accattacga aaagtttcaa ggttaggatt ggggcgatcc
     9481 gacttcacca ttccgatttc ttcgccgggt ttcagatcgt cgtaaataat gcctggctga
     9541 atggtaagct cgcgttcctt atccttgctg ccattaccat ccggttcata gctctgccca
     9601 tcgcctttcc ggatgtacat ccccagagca gcggcgatcc ttgctgcagt cagctcagaa
     9661 tcctcatact ctttcagggc actgaggcgg atcagcacac cggacaacaa agacgtcccg
     9721 cgcatctggt gcagacggcg aacaaattta agatgcagca ttcgctctgc atccacttct
     9781 ttggtttcca tctgccgtcc ggatacggga cggcttttat acaccagata tttttcggga
     9841 cgcccccagt catcaacaaa cacgccctga ttcagcctgt tgctctcatc actggtcatg
     9901 ggaataaagt ctggctcgag cgcctccagc cagaaatgaa caccggcaga aggcgtcagg
     9961 ctgtttatgc gcccggaaac catctgggca aacacctcac catcgcgcag ccaggtacgc
    10021 agcatcagac gttccagcat cggacgggta aactgcccgg tgacttccgg actgacagac
    10081 cattcactcc atcgggtgcg tatctccgct gccagatcac gggcaatggc cccattgcgt
    10141 aataccggat gtggctcgac aataatcccg tttttcccca ccacccgttc ttccagcttg
    10201 tcaaatacac caataaccag atcgtggttg ttatcaaggt aacgggcctg ctcacgtaac
    10261 gacacggccc cgtactggct taactggtcg gcagttcggt tttcccgccg ggctttgtgt
    10321 gtccgcgtcg tttttacggc ctcataagcc tggatcaccg cacgggaacg cagccttgcc
    10381 gctttccatc ctggtgaaaa aacgccaatc acatcatcaa gaattgccat cagaacctcg
    10441 ccagccggta cccgggatgc ccccgtcgtc gtgtaatcag agccgcaagg cggcgctccc
    10501 actcctgccg tccctgccgg atctcagata agttttccat ggtcatctgc tgaccattaa
    10561 aggtgacgga ttttccgtcc agcaccgcca tttcagcttc cgtataacgc tgaatcatgg
    10621 cttcgatatc attctggttc ataaccatcc tccggaagtc agccaggggt taacatcgtc
    10681 agttactgtt ttcttccgtt tttgtttttt aacaggcgtg gataccggtt ccggtgaggg
    10741 tgacggttcg gtactgtccg ggacactctc cagccaggtt tcccggctcg cccaccccgg
    10801 tgcatccggc cagcggatct tttcgtatcc atgcagaatg accagagcct cggcatacac
    10861 catcaggtca aaagcttcgt tggcaccgcg acccggctta ctccatttcc cgtcactact
    10921 ccgctcttca tacgtcagtt cgtcgtaaaa ccagctcccc agccagtcag ggaaatgcac
    10981 atagccggga cctggagagt cacgccataa cgcgttattc acccggtctt tcagtgcatc
    11041 cgtctgaaga agccagagcg gcacatcacc tgcggcctgc gcccgtcggc ccgttcgtcc
    11101 ggtgttatca gggaatgtac gggtgatcag ttttgcgcgc cggatgctgt cgcccttaaa
    11161 caggtaaata cgtttaccaa ggccatcacg acggcaacga cgccagaatt tataggcatt
    11221 atcagtgacc ccgtcttcac cgccggagtc caccgccatt gccatcagtc gcatttgttg
    11281 agaaggatcg gaggccagcg gccagctttt atgaaaaaca tccgtcagca ggacatccca
    11341 gtcttccgga tagctggccg gatcaattcg ctggctctca ccgtcgctgt caccgcgcaa
    11401 tgactgcgtg atgttgtaac gatcaataat ccagcgttcg ccacggctgc catagcccgt
    11461 tacctgaacc acaaaacggc gatgacgtcc cgcctgcaca tccactgtcg ccacaaggaa
    11521 attaacgcca tccggcacac tgctggaagg aactggctct gcccgctgct caagcagttc
    11581 actttttcgt tgctccatgc tggcgcgggg aagataaggt aatccccagt cggtattgat
    11641 aaccgtcttg agtgtttctt cacttccggt tgtctcgtat tcctgttctg cagtaagcag
    11701 tttgtaaacg agttgcgaga gtgtctggta agcagctgcc ggaccctcca tccagaatga
    11761 cgcaatacgt gagcgtcggg gatcaccata acgactacca tccgcattga tggattcacc
    11821 atcccgcaac cagaccccac gtccgttcag ctcacgtttt tgttcaggca taatccgtcc
    11881 tgaacaggaa ggacactgaa tataagccgc ctcacttgcc agcacgggat cggcaatatc
    11941 acggaaacca gcaaccacat cgccgcaggg ctgaaaatac tcaccacagt gtggacaggg
    12001 ccagtaccag cgacggcgat cgccacggtt atagagcgac agtatccccg tggttggtgg
    12061 agcctcatgc ggtgaagtcc gtcgccattt cacatccttc acatccctgc cgggggaact
    12121 ctccaccagc gtcataccac tggacataaa tgtggtggta cgttttgagg caagagagaa
    12181 ggcatccccc tcgccatcaa tatcttccgg aaaacggtca taatccgtca gcgccacgca
    12241 tttataatct gatgaggaca tgatattgac tgacggccag ccgattttca ggtagttgcc
    12301 agcaaggaat gttctgtcat aaacgttgtt gtcatttttg ttcggactca ggcgactgac
    12361 cacttccggg ctgacgcgaa acgttctggc gagtcgtttt ttggagtgtt cgcgggcttt
    12421 ttcctccgtc atctgaatga tcagcatatc cgcaggatcg caaatcacgt tgtaaatcac
    12481 ccagccgtca atcaggccga tagtcttgcc ggttcgtgcc gggccaacaa atatcactgc
    12541 gtcgtattca cgcgaggcca ggcagttcat cggctcaata acatacggtg ccaccagcgg
    12601 atcccacggg actgagttcc cggcccccat gggcacccgc atatactgag caacggcatc
    12661 agcaacccgc attcgtctcg gtgcgcgaag gatataacct gaatcggttc gtgctgcctt
    12721 tgcggtttcc tgattcagca ttactcctcc tgctgtaatt cctcctcatc atccgcacct
    12781 gcttcggtca cccgcagggc tatctgatcg cgcagatcat caataatgga ctgaacacgg
    12841 ctcacagcgg caggctgcag accacagtca cgttccagaa tatccggtaa tgtctccagc
    12901 acctgcacga ccgcttttgc ccagatggca aactcccgtc tgacatcact ggccggaatg
    12961 agttgtgccg tttcctgttc gaacttaaga cgctcacgtt cagactgata ccaggctttg
    13021 cgttcatgtg gatccatttc gccctcagca accggcggtg gtaaccccat aaattcagtc
    13081 agaatatcgg tcaaccggta tagcttgagt ttgtcatgtc caccagcggg acgaatgttt
    13141 ttcagtcttg ccacgacagt ctggcggtgc agaccagata aagccgccag ttgattaata
    13201 ttcagcacca ggtttttcaa ctcatgatcc atatttcctc cggagagctt taaacatgca
    13261 tcgtgcgaac aactttaaga aaacgcgttc gatgtcgaac aaaaaacact caattcgaca
    13321 tacaaaaaac aaataaccat taataatcaa taagatgcaa agatgatggt ggccgataaa
    13381 aatgcaaaaa ctagcctttt tccgcgacgc tcccgccccg tggcaggcca ccccaccggg
    13441 aggagccgtc agcctgaaag ccatgacgaa cgtctgatac agcccttgca tgaatggcat
    13501 cgggataatc cagaaaggaa tagcatcgac ccacaagaat ctgtgtgagt gtcctgtttc
    13561 ttccaccccc gcacaggact ggcgagcatg agggacaaac ccgcgaatca ttagcgcggt
    13621 aaaaacccgg tgtgcatcgt ttttgattat tcccgcacac tcgcgcagaa ggagttcccc
    13681 gtcgggctac ggtcatggtt aatgcgggaa tacagcgacg atacagcgca tgatgtgtca
    13741 ggcttgaata cctttatccg ttaaaaggga tatcagttaa gttatcccgt gtagggtata
    13801 agccattatc aaagccactc tgtagggagt ggcttttgta atggcaataa aaaagccccg
    13861 cgaatgcgag gctaaatcca ggtattttta atgactggct cttatctcaa cgcagcccct
    13921 taccgcgcgc cagatgctca atatcaagca tcagcaatga gatgtttaac catgattcac
    13981 tccagaagtg agcaccaccc tgtctacaga gccagatgtg aaggatgatg agtaaaatta
    14041 tcgctatcat cgaaggcatt gcgtcctgat gtattcctga agcgttctca gtgctgtttg
    14101 gtcgcggata attccgtccc ggataccgag aacgtttcgt ccagcaactg gagagagttc
    14161 gacggtggca tcattgccca tgccggaggc gctggaggtt tcgtctgagg atggcacagg
    14221 gcattttcct ttgacgaaca cccgaccacc attatcaagc ttgcgccgaa gagcatcatt
    14281 tttagctttc gcatcagcta actccttcgt gtatttagca tcgagtgcat cagcagcacg
    14341 ctggcgctgc tgcatgtcag taatggtggc ggtcgcctgc ttcagctcac tgactttttt
    14401 atcacgctgt tctttatagg cgatggcgtt atcacggtaa tgattaaccg cccatgactg
    14461 gcagacgatg atgcagataa ccagagcgga gataatcgcg gttactctgc tcattgttgc
    14521 ccccacaaac agacttcacg ctcaatctcg cggcgagtca tcagcccttt ccattgctta
    14581 ccgccagcgt atgtccagcg ccgtagctga tcacatgcgc ctttgatatc accctggttt
    14641 attttgcgaa gaagcgtcga tgttctgaaa ttaccagcgc ccacgttgta aacgaacgag
    14701 taaagagcgc cgcgcgttgt ttccggtata tcgactttga tgtacgggtt aatttgtctg
    14761 gcgaccgtgg caaggtcttt attcaggagg gctttgcatt ctgcttcggt atacgtttta
    14821 ccgggaatga tgtcttttcc ggtgtgtccg tgacatacag tccatacgcc aacgatatct
    14881 tcgtatggta tgtagctgac accttccagg ccatcgtcac cactcggacc agtgatgagc
    14941 acagacgcta tggcagcagc cccaccacca atagcagcag caacagcctt gcgtaatgat
    15001 ggcgacatta ttcacctctc gcagccttac gcttatcttc tttaatcttg aaataaaggt
    15061 ttgtcagata cgtcagcaag ccaaacacca agctacccga tacgcctatt gccacccact
    15121 gggatgggga gactttgtcc agcaactgca gtagccagta tcccgtcccc accgctgacg
    15181 tggtgtatga cacacctgtt gttatttttt ccatctggta cataccccgt ctcccgttat
    15241 ccggaagctg acaacaataa aaagccacca gttaattcct gatggccctg atgcataaac
    15301 gtcataatac ctgactgtta tgattgacaa taatgataat gtttatatag aaaggttccc
    15361 gatgtgtgtt acatatcatt tctccacggg gaatatcccc acgccagcgc agactctttt
    15421 acccgttctc ttctgcgctg gctctttttt attatgctgc tgcatttacc tctggcacca
    15481 tgctttctat ctcaacacaa tacgtggtac ttcttgtaac caatatcata acgattaatc
    15541 gacatagaat ttctcccgtg tacaggaaca gagttaaaaa gccggaaccg gaatcaaatc
    15601 acaggatgac catctgccag tggcaggtca taaaaaaaag gccgcgccat gcgcagccag
    15661 aactcacaag gaaaatgata gaaggaaata acattagtga tgtacgcatg gcgcctcccg
    15721 ctaagttctg caatgatcaa acagaactcg ctacgtgccc ttaaaactcg atcatttagc
    15781 ccctccaagg aggattcacc atgcggttga ttttttaata aacagtaaac aaaaaagtca
    15841 agaattattc attctgttct ttcatcatcg gccacagcaa taccacaatg ccgcagacca
    15901 gagcgccatc agtcagtacc aacattatcc tgctggtgaa atccatcatc accatcacta
    15961 aaagcaggat cacaacagca agcagacaca gtttataaaa caatgttcag gaaacgcatt
    16021 cagcatgcct aaggttctat tcctacgaat agccaacttg caac
//
LOCUS       DABSYT020000100        16008 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.100, whole genome shotgun sequence.
ACCESSION   DABSYT020000100 DABSYT020000000
VERSION     DABSYT020000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.100"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(284..955)
                     /gene="queE"
                     /locus_tag="ID725_RS19105"
                     /old_locus_tag="pgaptmp_003821"
     CDS             complement(284..955)
                     /gene="queE"
                     /locus_tag="ID725_RS19105"
                     /old_locus_tag="pgaptmp_003821"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGV7852322.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1110..1730
                     /locus_tag="ID725_RS19110"
                     /old_locus_tag="pgaptmp_003822"
     CDS             1110..1730
                     /locus_tag="ID725_RS19110"
                     /old_locus_tag="pgaptmp_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGV7852323.1"
                     /translation="MKRDLNTLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLN
                     QYQRRADLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQ
                     QAQAQVTRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYN
                     VTIRKFPAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            1744..2418
                     /locus_tag="ID725_RS19115"
                     /old_locus_tag="pgaptmp_003823"
     CDS             1744..2418
                     /locus_tag="ID725_RS19115"
                     /old_locus_tag="pgaptmp_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGV7852324.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            2652..3800
                     /locus_tag="ID725_RS19120"
                     /old_locus_tag="pgaptmp_003824"
     CDS             2652..3800
                     /locus_tag="ID725_RS19120"
                     /old_locus_tag="pgaptmp_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGV7852325.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            3797..4711
                     /locus_tag="ID725_RS19125"
                     /old_locus_tag="pgaptmp_003825"
     CDS             3797..4711
                     /locus_tag="ID725_RS19125"
                     /old_locus_tag="pgaptmp_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGV7852326.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(4771..6069)
                     /gene="eno"
                     /locus_tag="ID725_RS19130"
                     /old_locus_tag="pgaptmp_003826"
     CDS             complement(4771..6069)
                     /gene="eno"
                     /locus_tag="ID725_RS19130"
                     /old_locus_tag="pgaptmp_003826"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGV7852327.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(6157..7794)
                     /gene="pyrG"
                     /locus_tag="ID725_RS19135"
                     /old_locus_tag="pgaptmp_003827"
     CDS             complement(6157..7794)
                     /gene="pyrG"
                     /locus_tag="ID725_RS19135"
                     /old_locus_tag="pgaptmp_003827"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGV7852328.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(8022..8813)
                     /gene="mazG"
                     /locus_tag="ID725_RS19140"
                     /old_locus_tag="pgaptmp_003828"
     CDS             complement(8022..8813)
                     /gene="mazG"
                     /locus_tag="ID725_RS19140"
                     /old_locus_tag="pgaptmp_003828"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGV7852329.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(8884..9219)
                     /gene="mazF"
                     /locus_tag="ID725_RS19145"
                     /old_locus_tag="pgaptmp_003829"
     CDS             complement(8884..9219)
                     /gene="mazF"
                     /locus_tag="ID725_RS19145"
                     /old_locus_tag="pgaptmp_003829"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGV7852330.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(9219..9467)
                     /gene="mazE"
                     /locus_tag="ID725_RS19150"
                     /old_locus_tag="pgaptmp_003830"
     CDS             complement(9219..9467)
                     /gene="mazE"
                     /locus_tag="ID725_RS19150"
                     /old_locus_tag="pgaptmp_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGV7852331.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9545..11779)
                     /gene="relA"
                     /locus_tag="ID725_RS19155"
                     /old_locus_tag="pgaptmp_003831"
     CDS             complement(9545..11779)
                     /gene="relA"
                     /locus_tag="ID725_RS19155"
                     /old_locus_tag="pgaptmp_003831"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGV7852332.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(11827..13128)
                     /gene="rlmD"
                     /locus_tag="ID725_RS19160"
                     /old_locus_tag="pgaptmp_003832"
     CDS             complement(11827..13128)
                     /gene="rlmD"
                     /locus_tag="ID725_RS19160"
                     /old_locus_tag="pgaptmp_003832"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGV7852333.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            13185..15941
                     /gene="barA"
                     /locus_tag="ID725_RS19165"
                     /old_locus_tag="pgaptmp_003833"
     CDS             13185..15941
                     /gene="barA"
                     /locus_tag="ID725_RS19165"
                     /old_locus_tag="pgaptmp_003833"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGV7852334.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
BASE COUNT         3978 a         4061 c         3835 g         4134 t
ORIGIN      
        1 ctttacagta tcacacaaac aacacatcca ttatgttaaa gagcacttaa cccattgatt
       61 aaaaaggtaa atatttaaaa taactctata caaactaaaa tctaccaaac tttaccgcaa
      121 taattttcac tccagcgaaa aattaacgcc acagaatttg tagagaatga taatgattag
      181 tccgggatag gcaagatgga tgagttttca gagacgacaa agaatataaa tgagtagaag
      241 tctttacagg ttccacccat ttataacgct tataaatgtt taatcaggca atatttaaat
      301 atttatgggt ttgcatcgac aaacgccaat tacgcgcaat gcaggtttca atgcacaaac
      361 gtgtggcatc atccttttgg ctaatcggct gcagtgcaat gactcgcggt ttatcatcgg
      421 tcagcgtcgc caaaagttca tccagtgctt caatatcacg tacgcgtccc actggatgct
      481 tgatttcgtt ggctcgctcc agtgcctgtg acaacacttc atagccgccg cgcatgttca
      541 gctttggcga tacggtaacc caggtattcg gtgtacagcg tacttcatga gtaccactgg
      601 tttcgatctg gcagctaaaa ccgttctttt cgagcaggtc agtcagtggc agcaaatcat
      661 gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc ggtatatccc tggcgaccaa
      721 tgacagccag caagtcttca ctgctcgccg ccccccactt atcactctct ttggtcttcg
      781 ccagaatgct gaaaagggag acttcccgat cctcaagctt ttcccaggtg tgtttggtgt
      841 cgcaccaggc acagccaacc gggcatccct gtaaacgaat aaaaatggcg gggacgccgg
      901 taaagtaacc ctcaccttgc agggtctgga acatctcgtt aatcgggtac tgcatagcat
      961 tctctgtgaa gtggataatt gttaattatt gcagatcctg ccacaacaat catgtcttat
     1021 taacattctg ttacaggcag gtttatggca aaaagtggat tagatcataa agaataaaat
     1081 gaagcagagt attatgtttt aaggaattaa ttaaaaggga tttaaacacg ctcagaatat
     1141 tcatcgtaat tatatttatt ttcaatttat caggctgtgg atataacgat attcaaacct
     1201 atgatgagca ggttaacgcc tcctggtctg aggtattgaa tcaatatcaa cgtcgcgccg
     1261 atcttattcc taatcttgtc gccagcatta aaggttattc cagccatgaa cgagatgtac
     1321 tggaagccgt aacattggct cgtagccagg ctaatcgcgc cagtagcgat cttcagcaaa
     1381 ctccgggaga tgaacaaaaa ttacaagcct ggcaacaggc tcaagcgcag gtgactcgca
     1441 ctctcggtca gttaacgata attagtgagc gttaccctga gttaaaatct caggaactct
     1501 atcaaaattt aatggtacag cttgaaggaa gtgaaaatcg gattgccgtt gcgcgaggaa
     1561 gatatattaa agccattgaa caatataatg tcactatccg caaattcccc gctgtattaa
     1621 ccgcaaaggt catggattac acaccaaaga aaaattattt gccggatgat gtcgccgcag
     1681 taagtaaagc cccaacaata gattttagcc agaatgctaa tgctcattaa gcggggaagt
     1741 tctatgcgaa attttatctt ccttatggct ttcttctgtt catctgtgtt tgccacacaa
     1801 attccagtac ctgaatcacc caagtatgtg aatgacttaa ccggcacatt aacaaacagc
     1861 gaagtaaaca ccctgaccaa ccagattaaa gccctgacgc aaaaaaacta cgcacagttg
     1921 gtcgtgttag tggttgaaac aacaggcgat gaaaccatcg agcagtacgc gacgcgggta
     1981 ttcgatagct ggaaaccggg tgataaagac cgcgatgatg gcgttctact gttagtcgcc
     2041 tggcaggatc ataccgtgcg tattgaaatc ggttacggac tggaaggtat tattacggat
     2101 gcgcagtccg ggaaaattat tcgcaacagt attattcctg catttaaaaa aggcgacctg
     2161 gctggtggtt tgcaaaaggg aatcagcgat attgaaagtc gtctgacggg gaataattta
     2221 gcaacgataa caccgaccga tcaccctttg ccctttagcg gttggtgggc attgttagtc
     2281 tgggcgatag tactgacgtt tatttctgcc agaggatata tcaaaacgct gggagtaatt
     2341 tgcttcgcag ctattgtact ggcctttgtt ttacctatag caggcttcag cggaagctgg
     2401 ggagtactcg caacatgact ctgcttcgcc acaccgttcc tggctgtcgc catcgctttt
     2461 acccccttcg gcaaaaaagt tcgggatttg atgcgtgatg ccaaccagcc atcacgccat
     2521 acaagaagca attcttcctc ccactattca tcatcgtcat cctcttcatt cgacaatgac
     2581 aattttagcg gtggtggcgg ttcatctggt ggcggtggcg cttccgggcg ctggtaacaa
     2641 aaggaattga tatgcgtatt tttctcttac ttctgatgct atgcggtttt caggtattcg
     2701 ctgaacgatt gccttcagtg actgacttaa ccgggacatt aacgacagaa gaacaaactg
     2761 ctttaactca acaattacag actctcgagc agcaaaacca cttccaggtg gccgtgttgg
     2821 ttacgccgac cacagggggc aaaaacatta aacttgcagc ctcgcagaga tacggggtat
     2881 atcactggaa acccgttgga gagaagcgat atggcgaagg tatcttaatt ctcgtcgtat
     2941 ggccggaagg gctggcgtca atgaagattg gacatggact ggaagagatg ttgccgccgg
     3001 aacaagctgc gcaaattgtt cgttaccata tgcagccaga attcgaaaaa aacaatctct
     3061 ttgctggatt aacgggcgga attgaaagca tcgcacagtt tacccatatc aaagcgaacc
     3121 ttagcccttt ggatgcttta gccaatcatc tgtttgctaa tccgcaacgt tcgctgccat
     3181 gtcttgcctg gacactgctg atgatcgttg ccatcgttgt cctctggcta tttgccagcc
     3241 gcccacggcg tggcatatgg atgatatcga tgattacccc tatggtctgg tttttctcct
     3301 ttcaggacga tgtcatcatt cggcgggtat cggtcgtttg ttttagttta ttgttagcaa
     3361 caacctgcaa gcagcgtctc gcggtagttt ttaatatgtg ccgcgtattt cctatgatgc
     3421 tgaccccaaa aaataaaaac gctaaagtga aaaaacaaaa gaagactccg caggttcgtg
     3481 aatcaggacg aaattcattg tttgtcgtga tgctgataat tattgtggga tggtgcctgg
     3541 tgttcgccag taataaagat attgcattca tctcaacaat tattgggtta attgatcatt
     3601 ttatcggccc aataaccatt gctggcatcg ttctgctgat cattgtggcc aaatttactg
     3661 gcaatcttaa gctggggtca ggagaaaaaa gcaacaggaa aacggggaat cgtaatgctc
     3721 attctcgctc ttcgtcgaga aattctttcc gcggtggtgg cggttcttca ggaggcggcg
     3781 gttcatccgt ccgttggtga tcgtaatgcg cttaatcttt attctattca ctttgtggtg
     3841 ccttcctggg cttgcccagc aaattgctgt gccggaactg cgccaacagg tgaccgatat
     3901 tactggtacg ttaagcactt ctgaacaaca atcactgaca cagcaactgc aggatattac
     3961 acataaaaca cgggcgcagg tcgctgtatt agtagtgccg tccaccggag acgattccat
     4021 tgaacaatat gctacaaggg tttttgatag ctggaaactg ggagataagc agagaaacga
     4081 tggtatttta cttctggttg cctgggagga tcatgctgtt cgtattgagg ttggctacgg
     4141 gctggaaggg gtggttaccg acctgcaagc ggcgaagatt atcagggata tattgatccc
     4201 tgccttcaaa agtgatgacc tgatgggtgg attaacactg gcaagcgaaa atatcggcgc
     4261 gcttctgttg aatggtgaat taccggaaga caggggggat tattacagta tcaatccccc
     4321 tattccatta tcgcttgctg taattatatt gctggcggta ctttcatatt ttattgtttt
     4381 taccgatccg tcgaatttac cctggatcac tctcactggc gccatttacg ggatggtatt
     4441 cctctatgtt gccgagccag gaccatggac caatttaatc gttgcctgcg gtatgttaac
     4501 gccttttgcg atcgtaccac tggttatttt ttggcttatc gtcaataaaa agctacgcgc
     4561 caaatacaaa aaactcagta aagatagagc ttcaagaaaa ggttcttctt catcctcttc
     4621 cggtggcggc tcaagtggcg gaggctcagg cggcggattt agcggcgggg gcggttcttc
     4681 tggcggtggc ggcgcgtccg gtcgctggta actcaaccat aaaaaacgcc agcccgaagg
     4741 ctggcatttt taaatcagat aaagtcagac ttatgcctgg cctttgatct ctttacgacc
     4801 gttgtacggt gctttttcgc ccagagcttc ttcgatacga atcagctggt tgtatttagc
     4861 aacgcggtca gaacggctca tagaaccagt tttgatctgg cctgcagcag taccaacagc
     4921 caggtcagcg atggtagcgt cttcagtttc gccagaacgg tgagagataa ctgcagtgta
     4981 gccagcgtct ttcgccatct tgattgcagc cagagtttcg gtcagagaac cgatctggtt
     5041 gaatttgatc aggatggagt tagcgatacc tttttcgata ccttctttca ggatcttggt
     5101 gttggttacg aacaggtcgt caccaaccag ctggattttg tcgcccagaa ctttggtctg
     5161 gtatgcgaaa ccatcccagt cagattcgtc cagaccgtct tcgatggaaa cgatcgggta
     5221 ctgtttggtc agttcttcca ggaagtgagt gaattcttca gaggtgaatg ctttgttgcc
     5281 ttcgccagcc agaacgtatt taccgtcttt gtagaattca gaagctgcgc agtccatcgc
     5341 cagagtgatg tctttgccca gttcataacc agcagcttta acagcttcag cgataacagc
     5401 cagagcttca gcgttggaac ccaggttcgg cgcatagcca ccttcgtcac caacagcagt
     5461 gttcatgcct ttcgctttca gaacttttgc caggtgatgg aaaacttcag aacccatgcg
     5521 gatggcttct ttcacagttt tcgcgccaac cggctgaatc atgaattcct ggatatcaac
     5581 gttgttgtca gcgtgctcac caccgttgat gatgttcatc atcggaaccg gcatggagta
     5641 tttgcccgga gtgccgttca gttcagcgat gtgctcgtac agcggcatac ctttagcagc
     5701 tgcagcagct ttggcgttag ccagagatac agccaggatt gcgttcgcgc cgaatttgga
     5761 tttgttttcg gtgccgtcca ggtcgatcat gatcttgtca atgccagcct gatctttagc
     5821 atctttgcca atcagcgcct gagcgatcgg gccgtttacc gcagcaacag ctttggttac
     5881 gcctttaccc aggaaacggg atttgtcgcc atcgcgcagt tccagagctt cacgggaacc
     5941 agtagaagca cctgacggag cagctgccat accgacgaaa ccaccctcca gatgtacttc
     6001 ggcttcaaca gtcgggttac cacgggagtc gatgatttca cgaccgatga tttttacgat
     6061 tttggacatt aggttttcct caagtcacta gttaaactga aactccagac aaacaacgcg
     6121 tacccagggt acgcgttgcc gttctaactt ttttacttac ttcgcctgac gtttctggaa
     6181 ctcgctggcg gctttcacaa agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt
     6241 aaactccgga tggaactggc aagccacgaa ccacgggtga ttcggaactt cgatgatctc
     6301 gaccaactga tcatccccgg aacggcccgc aacgcgcaga cctgcatctt caatctgttt
     6361 caacagcatg ttgttgactt cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt
     6421 gtacagctgg cgaaccaggc tatcgtcaac caactggcac tgctgtgcgc cgagacgcat
     6481 ggtaccgccg agatcgctct tctcgctacg aacttcaacg ttgccgtttt catcgcgcca
     6541 ctcggtaatc agcgccacaa ccgggtactt acagtctggc acaaattccg tagagttggc
     6601 gttctccatg ttggcaacat ggcgagcgta atcaattaac gccacctgca tacccaggca
     6661 aatgcccaga taaggaatat tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc
     6721 tacgccacga tagccgaaac cgccaggtac gaggattgca tccagacctt taaggatttc
     6781 aacgccgcgc gtttcaacat cttgtgaatc gatcagtttg atgttgacgc tgacacgatt
     6841 cttcagccca ccgtgtttca gtgcttcaat cacggatttg taagcgtccg gcagttcaat
     6901 gtacttgccg accataccaa tggtgacttc acttaccgga ttcgcttctt cgaagataac
     6961 ctgttcccat tcggacagat ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata
     7021 atcgtccagc ccctgagatt tcaacaggcc cggaatttta tagatggaat cgacgtcttt
     7081 cagagaaata accgcttttt ccggaacatt acagaacaat gcaatcttcg cacgttcgtt
     7141 cgccggaacg gcgcgatctg aacgacaaat caggatgtca ggctggatac cgatggagag
     7201 cagctctttt acagagtgct gagtcggttt ggttttgact tcaccagacg ctgccatgta
     7261 cggcaccagc gtcaggtgca taaacagagt gtgctcacgg ccaatttcaa cagccatctg
     7321 gcgaatcgct tcgaggaacg gcagggattc gatatcacct actgtaccgc cgatttcgac
     7381 cagtactacg tcatgacctt cgccaccttc cagcacgcgc tctttgattg cgttagtgat
     7441 gtgcggaata acctgtacgg ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac
     7501 gtcagagtag atacgacccg tggtgaagtt gttgcggcgg ctcattttgg tacggatgaa
     7561 acgctcgtag tgccccaggt ccaggtcggt ttcagcgccg tcttcagtaa cgaacacttc
     7621 cccgtgttgg attgggctca tagtacctgg atcgacgttg atgtacggat ccagtttcat
     7681 gatggtcaca ttgaggccac gggcttcaag aatggctgcg agggaggctg cggcaatgcc
     7741 tttacccaga gaggatacga ccccgccggt cacaaaaata tagttcgttg tcatgctgaa
     7801 cctgagaagt taggttgaaa agacgatgga ataaccagga cgggaaagca gtatacccga
     7861 acatgacctg tgccacaaac tttcattatc cctcctcttc gccagcgcac tattgaaatc
     7921 aggagtgaga aaatagcccc tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca
     7981 tttaaaaaat gacacaaatg gcgcttgacc gcgtaattcc cttagagatc aatttcctgc
     8041 cgttttacct gttgccagac ttcttccatt gtttcgaggt caacacctgt catttccagt
     8101 ccacgcgcgg caacaatacg ctccacttcg cgaaaacgac gctcgaattt ttcgttcgct
     8161 ttttgcaatg cgatttccgc ttttgtccct aaatgacgag ccagattgac cgtggcaaac
     8221 agcaggtccc ccatttcctc ctccagttta gcctggtcga caacagcctg ccgcgcttcg
     8281 tacatcacct cgtcgatctc ttcgtagact ttatcgacta ccggaccaag cgtcgtccaa
     8341 tcgaagccaa cgttggcgca acgtttctgg attttttgcg cacgcattaa agccggtaaa
     8401 ctacgaggaa tatcgtccag cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg
     8461 atttgctccc aacgggcaag cacttcacta ctgttttcgg cagaactgtc agcaaaaaca
     8521 tgcggatggc gacgctctaa tttatcgcta atcgcagcgc aaatatcatt aaagtcaaag
     8581 cgcccttctt cctgagccat ttgggcgtaa aacaccacct ggaacagcag atcgccgagt
     8641 tcaccgcgca gatcgtcaaa atcttcacgg gcgatggcgt ccagcacttc gtaggtttct
     8701 tcaagggtgt aaggcgcaat ggtggcaaat gtctgctctt tatcccacgg gcagccgttt
     8761 tccgggtcgc gcaggcgctg cataatagtg agcaaacggt cgatttgatt cattagaatg
     8821 tcctgaaaat tgcgggtccg tcaggtggaa acctgtgacc agaatagaag tgagttagta
     8881 acactaccca atcagtacgt taattttggc tttaatgagt tgtaattcct ctggggcaac
     8941 cgttcctttc ttcgttgctc ctcttgcccg ccaggcgata ctttttacct gatcagctaa
     9001 cgctacgcca tcacgttcct gaccggataa aacaacttcg aacggatatc cttttgattg
     9061 cgttgtacaa ggaacacaca gacacatacc tgttttgttg ttgtacatga acggactcag
     9121 gacaacagcc ggacgatgtc cggcttgctc gctacctttt gtcgggtcaa aatcaaccca
     9181 aatcagatcg cccatatcgg gtacgtatcg gcttaccatt accagacttc cttatctttc
     9241 ggctctcccc agtcgatatt ctcgtggagg ttttccggcg tgatgtcgtt gaccagttca
     9301 gcaagcgtaa atacgggctc tttacgcact ggctcaataa ttaatttgcc atccaccagg
     9361 tcaatcttca cttcatcatc aatattcaga ttgagcgcct gcattaacgt agccgggatc
     9421 cgcaccgccg gtgaatttcc ccaacgcttt acgctactgt ggatcataac cctttcctca
     9481 aaccactatc atatgtagat acagtatata tcaatctaca ttgtagatac gagcaaattt
     9541 cggcctaact cccgtgcaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac
     9601 ccagcacgcg ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca
     9661 gttgctgttt ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga
     9721 gaatggtcgt gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca
     9781 gcgaatatcc ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat
     9841 gggagcgcag ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct
     9901 gggtaatgaa gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt
     9961 gcatcaggtt accaacacct tcaaccacta cacgaccgtt atctttactg cggttttgcg
    10021 gcgtgtagct tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg
    10081 gcttattaaa ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac
    10141 caatcgccgc cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt
    10201 ctgcttcttt caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag
    10261 ccagaatgtt tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac
    10321 ggctggttgt gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt
    10381 tctgggtgat aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc
    10441 gcccgccaat ttttgccccg atacagcggt gtccgacatc actgtggatg tggtaagcga
    10501 agtccagcgg cgttgatccc gcaggcaaat caacgacatc acctttaggc gtaaagacgt
    10561 acacccggtc gtcaaagacc tggctgcgta cttcgtcgag catttcgccg gaatcagcca
    10621 tctcttcctg ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac
    10681 gtgcgccgcc agcagccgcg ccctctttat atttccagtg cgcagcaaca cccaactctg
    10741 catcttcatg catctgtttg gtgcggattt ggatctcaac ggtttttcca cccggcccca
    10801 gaaccacggt atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa
    10861 actcatccgg caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct
    10921 gtaaacgctc ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt
    10981 tctttttctg cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag
    11041 ctttaacgcc ttcagctttc atctcagcgc gcagatgacc gacaaactct tcgatatagt
    11101 gttcgcggtc gagacggcgt tcatgcaaca gttttgcgat gcgtttgtat tcggtcgggt
    11161 ggagataacg gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt
    11221 ttgccaacgg cgcgtagata ttggtgcact cttttgccgc cagtacgcgt tcatcttccg
    11281 gcgcatcttt tacttcacgc agatgagcaa tacgctccgc cagtttgatg accacgcagc
    11341 gaaaatcatc gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa
    11401 cagaatcagt gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa
    11461 taaggttaac gaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg
    11521 catcagccag agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg
    11581 agaggatctc caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct
    11641 gcgtctgttg cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg
    11701 taatacccag acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg
    11761 cacttcttac cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa
    11821 tcgcaactat ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa
    11881 catatccagc atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc
    11941 ccgagccagc gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat
    12001 aatttgctgc ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa
    12061 gccgtttttc gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt
    12121 cacattctgt aagcagttaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac
    12181 accttctaca cccaccacac tggcagcttg tgtcgccaat ggtagtgtaa agttgcccat
    12241 accgcagaac agatccagta cgcgatcttc aggttccacc tccagccatt ccaacgcacg
    12301 cgctaccatt ttttggttca cacccgcatt aacctgaata aaatcgcgcg ggctaaaagt
    12361 taagcgcaac ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc
    12421 actatcgggg gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc
    12481 gcgatctgcc gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc
    12541 ctgtaccagt tcaacatgac caagatggcg catagcttgt aagctgccca ggcatgccct
    12601 gactttgggc agcaatgctt caagttgggg cactaaaatg ggacattgtt taacgtcgac
    12661 aatgtcactg gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta
    12721 gttcagactt aaacgcgcgc gacggcgata gccccaggga acatcggcga tcacttcaga
    12781 gacttcgtgt ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac
    12841 gctggcgtgt tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc
    12901 gcgttccggg ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc
    12961 ctcagtaaca gtaacttccg cgttttcctg cggcaataat ccggggataa atagcgtttt
    13021 gccgttatgt cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat
    13081 gatctgacgc gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt
    13141 ctcaatttaa cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc
    13201 tgcgcgcacg catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga
    13261 gtatcttttt cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg
    13321 ccagcattat tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg
    13381 aatctatcgg tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga
    13441 tttcggttta tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct
    13501 catcaatgca gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg
    13561 gcgatattat gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc
    13621 ccagtagcga tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc
    13681 ttaaatcggt tcgcttgcag caatataaag agatctttat ttccagcgtg atgatgctgt
    13741 tttgtatcgg tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga
    13801 ttcgcaacat ggtgaatact gtcgaccgca tccgtcgcgg gcaactcgac agccgagtgg
    13861 aaggatttat gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt
    13921 cgctggctgc ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc
    13981 gtgaaacgct ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg
    14041 cccaggaagc ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta
    14101 caccactgaa tggtgttatt ggttttaccc gcctgacgct gaaaacagaa ttaacaccaa
    14161 cgcagcgcga tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta
    14221 atgatgttct cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat
    14281 tcccactacg cagcacgctg gatgaagtcg ttactctgct ggcacattct tctcacgata
    14341 aagggttgga actgacgctc aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc
    14401 cactgcgatt acagcaaatc atcactaacc tggtggggaa tgcaattaaa ttcaccgaga
    14461 atggcaacat tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg
    14521 aagtgcagat tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc
    14581 aggccttccg acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc
    14641 tggtgattac acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc
    14701 cgaatcgcgg ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta
    14761 ttgaagggcc atccacccag tgcctcgccg gtaaacgcct ggcctatgtc gaaccaaact
    14821 ccgcagcagc gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata
    14881 gcccaacgtt ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg
    14941 tgaccttccg cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga
    15001 ccgatttcct gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc
    15061 aagatggtat cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc
    15121 tgacggaatt ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc
    15181 tggcaatgac agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat
    15241 tgctggaaga tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcggttgaac
    15301 gggcgaaaca gatgccgttc gatttgatct taatggatat tcaaatgcct gacatggatg
    15361 gcattcgggc ctgcgagctc atccaccagc tcccccatca gcaacaaacg ccggttatcg
    15421 cggtaacggc gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg
    15481 attatctggc gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc
    15541 ctggcagcgg tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc
    15601 cgaatgcgac cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag
    15661 cgcgcgatat gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg
    15721 aacagctggt tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca
    15781 gttgcggcta tagcggtgtg ccgcgtatga agaatctctg ccaacttatc gaacaacagc
    15841 tacgtagcgg tactaaagaa gaagatttgg aaccggagct gctggaactg ttggacgaga
    15901 tggataatgt cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag
    15961 caatcggctt tttatgacgc tgttttcaga ttggtgacaa agtgcctg
//
LOCUS       DABSYT020000101        15925 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.101, whole genome shotgun sequence.
ACCESSION   DABSYT020000101 DABSYT020000000
VERSION     DABSYT020000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.101"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(50..1390)
                     /gene="gudD"
                     /locus_tag="ID725_RS19170"
                     /old_locus_tag="pgaptmp_003834"
     CDS             complement(50..1390)
                     /gene="gudD"
                     /locus_tag="ID725_RS19170"
                     /old_locus_tag="pgaptmp_003834"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGV7852335.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(1411..2751)
                     /gene="gudX"
                     /locus_tag="ID725_RS19175"
                     /old_locus_tag="pgaptmp_003835"
                     /pseudo
     CDS             complement(1411..2751)
                     /gene="gudX"
                     /locus_tag="ID725_RS19175"
                     /old_locus_tag="pgaptmp_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(2753..4105)
                     /gene="gudP"
                     /locus_tag="ID725_RS19180"
                     /old_locus_tag="pgaptmp_003836"
     CDS             complement(2753..4105)
                     /gene="gudP"
                     /locus_tag="ID725_RS19180"
                     /old_locus_tag="pgaptmp_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGV7852336.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(4540..4989)
                     /gene="yqcA"
                     /locus_tag="ID725_RS19185"
                     /old_locus_tag="pgaptmp_003837"
     CDS             complement(4540..4989)
                     /gene="yqcA"
                     /locus_tag="ID725_RS19185"
                     /old_locus_tag="pgaptmp_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGV7852337.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(5007..5789)
                     /gene="truC"
                     /locus_tag="ID725_RS19190"
                     /old_locus_tag="pgaptmp_003838"
     CDS             complement(5007..5789)
                     /gene="truC"
                     /locus_tag="ID725_RS19190"
                     /old_locus_tag="pgaptmp_003838"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGV7852338.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(5789..6118)
                     /gene="yqcC"
                     /locus_tag="ID725_RS19195"
                     /old_locus_tag="pgaptmp_003839"
     CDS             complement(5789..6118)
                     /gene="yqcC"
                     /locus_tag="ID725_RS19195"
                     /old_locus_tag="pgaptmp_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGV7852339.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(6740..7285)
                     /gene="syd"
                     /locus_tag="ID725_RS19200"
                     /old_locus_tag="pgaptmp_003840"
     CDS             complement(6740..7285)
                     /gene="syd"
                     /locus_tag="ID725_RS19200"
                     /old_locus_tag="pgaptmp_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGV7852340.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            7353..8201
                     /gene="queF"
                     /locus_tag="ID725_RS19205"
                     /old_locus_tag="pgaptmp_003841"
     CDS             7353..8201
                     /gene="queF"
                     /locus_tag="ID725_RS19205"
                     /old_locus_tag="pgaptmp_003841"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGV7852341.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            8313..9677
                     /gene="ppnN"
                     /locus_tag="ID725_RS19210"
                     /old_locus_tag="pgaptmp_003842"
     CDS             8313..9677
                     /gene="ppnN"
                     /locus_tag="ID725_RS19210"
                     /old_locus_tag="pgaptmp_003842"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGV7852342.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            10234..11523
                     /gene="sdaC"
                     /locus_tag="ID725_RS19215"
                     /old_locus_tag="pgaptmp_003843"
     CDS             10234..11523
                     /gene="sdaC"
                     /locus_tag="ID725_RS19215"
                     /old_locus_tag="pgaptmp_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGV7852343.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            11581..12948
                     /gene="sdaB"
                     /locus_tag="ID725_RS19220"
                     /old_locus_tag="pgaptmp_003844"
     CDS             11581..12948
                     /gene="sdaB"
                     /locus_tag="ID725_RS19220"
                     /old_locus_tag="pgaptmp_003844"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGV7852344.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            13060..13815
                     /gene="xni"
                     /locus_tag="ID725_RS19225"
                     /old_locus_tag="pgaptmp_003845"
     CDS             13060..13815
                     /gene="xni"
                     /locus_tag="ID725_RS19225"
                     /old_locus_tag="pgaptmp_003845"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGV7852345.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(13870..15018)
                     /gene="fucO"
                     /locus_tag="ID725_RS19230"
                     /old_locus_tag="pgaptmp_003846"
     CDS             complement(13870..15018)
                     /gene="fucO"
                     /locus_tag="ID725_RS19230"
                     /old_locus_tag="pgaptmp_003846"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGV7852346.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15046..15693)
                     /gene="fucA"
                     /locus_tag="ID725_RS19235"
                     /old_locus_tag="pgaptmp_003847"
     CDS             complement(15046..15693)
                     /gene="fucA"
                     /locus_tag="ID725_RS19235"
                     /old_locus_tag="pgaptmp_003847"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGV7852347.1"
                     /translation="MERNKLARQIIETCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         3878 a         4303 c         3814 g         3930 t
ORIGIN      
        1 gcatcagtct caaaccggct ccagatagag ccggttttgg ttttttgtct taacgcacca
       61 tgcacgggcg cttgttatcg aacgtccagc caggaatcag atactgcatt cccatcgcat
      121 cgtcacgcgc gccaagcccg tgtttctgat acagctcatg ggctttcatc acttgatcca
      181 tatcgatttc tacacccagc cccggttttt ctggcacctg taccagcccg cctttgatct
      241 caaacggttc tttggtcagg cgctgattac cttcctgcca gatccagtgc gtatcaatag
      301 cagtaatttt acccggtgca gcggcggcaa catgggtaaa catcgccagg gaaatatcga
      361 agtggttgtt agagtgtgaa ccccaggtca ggccaaattc atggcacatt tgcgccacac
      421 gtaccgaacc ttgcattgtc cagaaatgcg gatccgccag cgggatatca acggattgta
      481 acgagagcgt atggcccatc tgccgccagt cggtggcgat catattggtt gcagtcggca
      541 ggccagtcgc gcgacggaac tctgccatca cttcacgccc ggagaaacct tgctccgcac
      601 cacacggatc ttctgcataa gccagcgaac ctttcaggta tttgccgatt ttaatcgctt
      661 cgttcagcga ccaggcaccg ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg
      721 ccagtgccac aatagactcg gcctcttctt ccccggccag tacaccgcct ttcagtttga
      781 aatcgttgaa gccatatttt tcatacgccg cttccgccag gcgcaccacc gcatccggcg
      841 tcatcgcttc ttcatgacgc aggcgatacc agtcgcatga gtcatccggc tggctttgat
      901 acggcagagg tgtggctttg cgattaccga cgaagaacag ataaccgagc atttcgactt
      961 cgctacgctg ttgaccatcg cccagcaggg atgccacgtt aacccccaga tgctgcccca
     1021 gcagatccag cattgccgct tctatcccgg taactacatg aatagtggta cgcaaatcaa
     1081 atgtctgcaa accgcgcccg ccggcatcac gatcggcaaa agtattacgc accagcgtca
     1141 gaacgttttt gtattcaccc agcgttttac ccactaccag cggaattgca tcttccagcg
     1201 ttttacggat tttctcgccg ccgggaattt cccctacgcc agtgtgacca gaattatctt
     1261 tgataatcac aatattacgc gtgaagaacg gtgcgtgtgc accactcaga ttcatcagca
     1321 tactgtcatg gcccgccacc gggatgacct gcatttcagt aacaacaggc gtcgtaaatt
     1381 gagaactcat atttatgtcc tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg
     1441 gtcaaaggtc cagccgggga tcaggtactg catcgggcct gcgtcgttac gcgcaccgcc
     1501 aggcagacgt ttataggcct catgtgcctt ttgtacctgt tcccagtcca gttccacgcc
     1561 cagaccgggc gcatcaggaa cggcaatttt tccgttttta atctccagcg gattttgggt
     1621 caggcgacaa tcgccctcct gccaaatcca gtgggtatcg atagcggtag gattacccgg
     1681 tgccgccgcg cccacatggg taaacatcgc cagagagata tcgaaatggt tgttggaatg
     1741 gcagccccag gtcagccccc agtcgtcgca aagctgcgcc actcggactg caccggaaag
     1801 cgtccagaag tgcggatcgg caagtggaat atctaccgca ttgagcatca ccgcatgacc
     1861 catttcgcgc cagttggtgg cgatcatatt agtcgcgacg ggcaagccgg tcgcccgccg
     1921 aaactccgcc atcacttcac gtccggagaa gccctgttct gcgccgcatg gatcttcggc
     1981 ataggtaaga acatcattca gccctttgca cagagaaatg gcttcatcaa gcagccatgc
     2041 accgttggga tcaacggtaa tccgcgcatc cgggaagcgt ttcttcaatg cacgaacagt
     2101 gtcgatttct tgctcgccag gtaacacgcc gcccttaagt ttgaaatctt taaagccgta
     2161 gcgatcctgt gaggcttccg ccagacgcac aacggcttcg ctgttcatcg ctttctgatg
     2221 gcgcaactga taccactcat ggttgcccgg cgtattttcc agataaggaa gatcggtttt
     2281 ggtccgatca ccgatataaa acagataacc gaggacggta atagcatcgc gttgcttgcc
     2341 tggccctaac agttcgcaga ccggaacatt cagcgcctta cctagcaggt caagcaaggc
     2401 ggcttccagc gccgccacgg cgttaacgcg caattcaaag gtccaggccc ctttaccgaa
     2461 ggtatcaaaa tcggctgctt gattaccttt atgcacctgc tgaaccactt tattcaggcg
     2521 cgcaacttcc tagcccagca ccatcggaat agcatcgacc aatgtctgat aaatcacctc
     2581 tccgcctggc gcttcaccaa tgccggtatg cccggcgtta tcggtgagta ccacaatatt
     2641 gcgagtgaaa tatgcgttat gcgccccacc aatattaagc aacatgctgt cgtacccggc
     2701 gaccggaatg actttcatat cagtaataac aggactggat tgtgtcgtca tcttattgcc
     2761 ccacgacagg tttcagctcg atacgcttga tatcgcccac cagcaccagg tagctcagta
     2821 ccgcgattaa ggcatgaaca ccaacataaa tcagcgcccc attaaacgag ccagtcgtgc
     2881 caacgatata accaattgcg attggcgtga cgatgccaga aatgttaccg aacatgttga
     2941 acaggccacc ggaaagaccg ctgatctctt ttggcgcggt atctgccatt actgcccaac
     3001 ccagcgcccc aatgccctta ccgaagaagg ccagcgccat aaagccgatg atcatccact
     3061 caacgttgac gtagttgcag aacaccatca ccatcgacag caacatgccc attacgatcg
     3121 gtgttttacg cgcaatgttc agcgatcccg tgcggcgcat cagccagtcg gaaataatcc
     3181 cacccagcac accaccgata aaaccacaaa ccgccggaac ggaagccaca aagcccgctt
     3241 tcagaatcga catcccacgc gcctgcacca gataaaccgg aaaccaggta atgaaaaagt
     3301 aagtcagggc gttgatacag tactgaccga tataaacgcc gatcatcatc cgtgacccga
     3361 gcagttgttt gatctgcccc cacttcacgc tgaacggcac tttaactttg gtgttttgct
     3421 gatccatatt gatcagcgcg ccacccgcgg cgatgtactc cagctctttc tgatttaccc
     3481 ccggatgttg atttggctcg tggatgactt tcaaccagat aaagctgatg gcaatgccca
     3541 gaccgcccat aaagaagaag acgtgtgacc agcccacttc atgcgtcagc cagcccataa
     3601 tcggcgcgaa gatcaccgtt gcgaagtatt gagcggagtt aaaaatcgac accgccgttc
     3661 ccctttcctg cgccggaaac caggccgcaa caatgcgact gttgccgggg aaagatggcg
     3721 cttcagcaag cccgaccagg aagcgcagag taaacagggc aacgataatg ccgaatccac
     3781 taaagatatc gacgaagcct tgcagcaagg taaacatcga ccagataaag atcgaccaga
     3841 agtagacgcg ttttgaacca aaacgatcca gcaaccaacc accagggatc tgcccgataa
     3901 cataagccca tgagaaggca gagaacacat agcccattcc cacgggatca aggccgatat
     3961 ctttggccat ttccgaaccg gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg
     4021 tgacgataaa caacatcacc actatccagt aacgagcatt cgtgcgtttt tccacactgc
     4081 tcgcagcctg acttaaagaa ctcattgttg cactcctgaa aattcgcgtt agccacgctc
     4141 actctggact gcgacatcgc caggaaatca gaggtgacgt agggtgtttt ttgccgtttt
     4201 tataggtcgt tcgccgaata cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg
     4261 cgccatagcg gctcaccgtg caacaacaca acattaatgc gttcaatgag gcccgatttt
     4321 ggcattagcc ctggacggtg gaatccactt cacggaaatg aaaacaagaa caagaaagga
     4381 agggttaaaa cgaagaaata aagaagagta tgaaatggat cgcttgactc caggcaaacg
     4441 ccagtaaaaa tccgcgctat gaagcagttt ttactggcat ttgcctgaaa agattcgatt
     4501 cagcaccgct aaaacgacat ttaccgctcg ctgaacatat caggacaaca gcgtgcccca
     4561 ctgttcgacc cacggatttg attccgtttc cggttccggg ttctcactgg cgtcaatcaa
     4621 cagcatttcg ccaacccgct gagcgctctg ttcctgcagc aaggcatcga actgtttgcc
     4681 gccattgcag aaattcacat aactactgtc gccgagcgca atcacgccat aacgcagatt
     4741 cggctggaag cccagactat ctttgattcc ctgaaagagc ggcacaatgc tatcaggaag
     4801 gtcgccctgc ccggtcgtgg acgtaaccac cagaacatac ttatcctgat agggcagcca
     4861 gtcgcttaat tcaggatctt caaataccgt tgctttgtgg ccctgtgcgg tcagaattgc
     4921 ttctgcttct tccgccacta acagcgaatt tccgtacatg gtgccgacaa aaataccaat
     4981 ttccgccata ccgttttctc cctggattag gaacttctct ctccatcctg acccgatggc
     5041 gcactgaact caaccctttc attttcagga agcagaccgc gccagccaaa ttgtgataac
     5101 gcctgcatcc aggtgtcgtc caggcccgcg tgaatagtca gcggctcacc agtaaaagga
     5161 tgcgtcaatg acaactgact ggcatgcagc attaaccgct gaagaccaaa atgctcagca
     5221 ccgctgcgat tctggcgtaa atcgccatgt ttgctatcgc caataatcgg atgacgcaaa
     5281 tgggcaagat gtcggcgaag ctgatgtttg cgtccggttt tcggctccag ttccaccagg
     5341 ccgtagcgcg tggtcgggta acgtccggtc gctaccggca tttctacggt cgccagaccg
     5401 cgataatgcg tcactgctgg ctgcgggcct ttatcttcgc gggcaaattt atcagcgatt
     5461 ttgtccagtt cttccaccag tggataatcc agcaccgctt cttccatcaa ccagccgcgc
     5521 acaatcgcat ggtaacgttt ctggatttgg tgctgttcaa actgttgtgc cagcagccgt
     5581 ccggcctcgc tggataatcc catcaacaac acaccagaag tgggtcggtc cagacgatga
     5641 gcagtaaaaa catgctggcc tatctggtca cgcacggttt gcatgaccac tactttctcg
     5701 tcgcgatcca gccagctacg gtgaaccagc cagccggagg gtttatttac cgcaaccagc
     5761 cattcatcct gatagagtat ttccagcatt agctcgcatc atccgcaaaa agagcatcca
     5821 gtttttccaa ctcagccaga ataagcgcgc gttgcggatg gtccgtcgcc agcgccattt
     5881 cataataggg tgcaacggca aaagcgcccg gtaacggctg gttgttattt aacaaatcgt
     5941 gcattcgcgg gatcagcacc cactgcaacc actccagtgg ttccatggtg tccataaaga
     6001 acggttgggt actattaaat tgatgcggct ggggttcatc gtttcgccag tgctgatgtt
     6061 cacgcagtaa cgcttcaagc gcctgcaact ggagacgaac gcggtcatga gtggtcataa
     6121 agcaacctca ataagaaaaa ctgccgcgaa ggatagcagg aataaaaaaa gggagcactg
     6181 tattcacagt gctcccggtt cgtttcgcag cattccagct acttttgttg ctccctgctc
     6241 atccttgaca acttttcctc tggccatgcg gccaatcgtt catcctgaac tattgcttcc
     6301 tgctcacacc accccgatgt gatacttcat cctgaagtgt cccaggcctt cctgacccac
     6361 cgaatcatcc tgaccggttc tcattctcca tcctggaggt gtcctttaac gcgtcctgcg
     6421 tcatcctctt cgcttcatcc agaagccttt ccctgaaaca ccatcctggt gtgtccttca
     6481 gaagtgtcat catcctgatg ttcacttcgt tgtctgactc cctgtcgacg aagatagaat
     6541 cgtctttttc gcgaactctt acaaggcgct tacagacaat gccttaagga aaatttcata
     6601 tgaaagttag gaattaacaa gcaacatgtt gtttttaaat gaaaataacg attcttatct
     6661 ttttaagtat tcgcttattt cctggcgatc tcctacagag tctgtaagag atctctcaca
     6721 accagatggg gtatttggat tacagaagag gcttaagttg attaaggaat tccgcaagat
     6781 ttggggctaa atgcgtccgt ttacgtgttc ccagcgtctc tttacacact tcaccggaga
     6841 gattacacac cgagatgacc tctaactctt cttccagcgt cgcgataaat agcgtgggtg
     6901 gcagttttag ccttttctga gtcaccagat gaccaatcag attttcctgc acccgacgga
     6961 agtcgtcttc actccaggtt tgcagcaatg tcagtttgat atcgccaaac tgcgcgtgca
     7021 tatccccggc aaactgagtc gtataaaagg tatgaattgt gggttgtatc acaatatcaa
     7081 aagcgcgttc aaccgcgttt acattttgtt ccccggtaaa cggctggggt tgccagtata
     7141 cggcatcttc agtagttgaa ataatgcacg gtgaaggaac gccgtacagt tcctcactta
     7201 acggccagct tttatgctct tcatgccacg catcgcagta gcgtgcagta aaatctttca
     7261 gggcttgtgc ggtcaaatcg tccactaatt tctctcttca cttaaaccag atacacttgt
     7321 cgtttagttt atctggttta tgacggtgaa acatgtcttc ttatgcaaac catcaggcac
     7381 ttgcgggcct gactcttgga aaatcaaccg attaccggga tacctatgac gccagcctac
     7441 tgcaaggcgt tccacgcagc ctgaatcgcg acccgctggg tctgaaagcg gataacctgc
     7501 cttttcaagg tacggatatc tggacgctgt atgaactttc ctggctgaat gcgaaaggtt
     7561 tgccgcaggt cgctgtcggt catgttgaac ttgattacac cagcgtaaat ctgattgagt
     7621 cgaagagttt taagctctat ctcaacagtt ttaaccagac gcgttttaat aactgggatg
     7681 aggtgcgcca gacgctggag cgcgacttaa gcacttgcgc tcagggtgag gttagcgtgg
     7741 cgttatatcg tcttgatgaa ctggaaggcc agccgatagg tcatttcaat ggcacttgca
     7801 ttgatgacca ggatatcact atcgataact atgaattcac tactgactat ctggagaatg
     7861 ccaccagtgg tgaaaaagta gtggaagaga cgctcgtcag ccacctgctg aaatcaaact
     7921 gcctgatcac ccatcaacca gattggggtt cgatccaaat tcagtatcgt ggacgccaaa
     7981 ttgacagaga aaaactgctg cgttacctgg tctcattccg tcatcacaac gagttccacg
     8041 aacagtgcgt ggaacgcatc tttaatgacc tgttacgctt ctgccagcca gagaaattga
     8101 gcgtttacgc acgttatacc cgtcgtggcg gtctggacat taacccgtgg cgcagtaata
     8161 gcgattttgt cccatcgacc acaagactgg ttcggcaata aattttttct caattttgcg
     8221 tgctggattc acgcagaagg ttgtgaaagg tcatcaggca gggctattgt aatcaaaggg
     8281 aatgacgata ttcgtcccat aaggagtttt tcttgattac acatattagc ccgcttggct
     8341 ccatggatat gttgtcgcag ctggaagtgg atatgcttaa acgcaccgcc agcagcgacc
     8401 tctatcaact gtttcgcaac tgttcacttg ccgtactgaa ctccggtagt ttgaccgata
     8461 acagcaaaga attgctgtct cgttttgaaa atttcgatat taacgtcttg cgccgtgaac
     8521 gcggcgtaaa gctggaactg attaatcccc cggaagaggc ttttgtcgat gggcgaatta
     8581 ttcgcgcttt gcaggccaac ttgttcgcgg ttctgcgaga cattctcttc gtttacgggc
     8641 aaatccataa taccgttcgt tttcccaacc tgaatctcga caactccgtc cacatcacta
     8701 acctggtctt ttccatcttg cgtaacgctc gcgcgctgca tgtgggtgaa gcgccaaata
     8761 tggtggtctg ctggggcggt cactcaatta acgaaaatga gtatttgtat gcccgtcgcg
     8821 tcggaaacca gctgggcctg cgtgagctga atatctgcac cggctgtggt ccgggagcga
     8881 tggaagcgcc gatgaaaggt gctgcggtcg gacacgcgca gcagcgttac aaagacagtc
     8941 gttttattgg tatgacagag ccgtcgatta tcgccgctga accgcctaac ccgctggtca
     9001 acgaattgat catcatgccg gatatcgaaa aacgtctgga agcgtttgtc cgtatcgctc
     9061 acggcatcat tatcttccct ggcggtgtgg gtacggcaga agagttgctg tatttgctgg
     9121 gaattttaat gaacccggcc aacaaagatc aggttttacc attgatcctc accggcccga
     9181 aagagagcgc cgactacttc cgcgtactgg acgagtttgt cgtacatacg ctgggcgaaa
     9241 acgcgcgccg ccattaccgc ataatcattg atgacgccgc tgaagtcgcc cgtcagatga
     9301 aaaaatcgat gccgctggtg aaagaaaatc gccgtgatac aggcgatgcc tacagcttta
     9361 actggtcaat gcgcattgcg ccagatttgc aaatgccatt tgagccgtct cacgagaata
     9421 tggctaatct gaagctttac ccggatcaac ctgttgaagt gctggctgcc gatctgcgcc
     9481 gtgcgttctc cggtattgtg gcgggtaacg taaaagaagt cggtattcgc gccattgaag
     9541 agtttggtcc ttacaaaatc aacggcgata aagagattat gcgtcgtatg gacgacctgc
     9601 tacagggttt tgttgcccag catcgtatga agttgccagg ctcagcctac atcccttgct
     9661 acgaaatctg cacgtaatct cccctccccc ggtgacgttt gttgccgggt tttctcgttt
     9721 ttggtcactt actcatcaac tcatttcatt tgttatatga atgtttctta ccaccctcac
     9781 gcgacaaata tcatcacagt taatatgtca tacaatttat attgcaacgc aaacgtttcc
     9841 ctattttcat aaaccgtatt ttatcgctat gaaaaagaaa ttatcgccat gattaactaa
     9901 aagtattgat tttttcagtt caacctacat atattgcgcg ccccggaaga agtcagatgt
     9961 cgtttaatgg gcaaatattg cccttaaatt ctcttttact tttgatttac agagtaaagc
    10021 gttgggataa tctatcttcc aagtagatta ttgtatttgg gatcaagatc actgatagat
    10081 acataacttg tgtgtatctt tccgccctca aattattacg gcggtaaatg attaagccat
    10141 cgccgataga cagatttcat ttttacggtc aggcaccttc ccgggctgaa ctggctaaaa
    10201 gctgaattat ttgcattcct ccaggagaaa tagatggaaa cgactcaaac cagcacgatt
    10261 gcgtcgaaag actctcgtag tgcctggcgc aagacagaca ccatgtggat gctgggcctt
    10321 tacggcacgg caatcggcgc gggcgtgcta ttcctgccaa tcaacgccgg tgttggcggt
    10381 atgatcccgc tgatcatcat ggctatcctt gcgttcccga tgacgttttt tgctcaccgc
    10441 ggcctgactc gcttcgtact gtctggtaaa aacccgggcg aagacattac cgaggttgta
    10501 gaagaacact ttggtattgg cgcaggtaaa ctgattaccc tgctctactt cttcgctatc
    10561 tacccgatcc tgctggttta tagcgtggca atcaccaata cagttgaaag cttcatgtct
    10621 caccagctgg gtatgacacc accgccgcgt gcgattctgt cgctgatcct gatcgtgggt
    10681 atgatgacca tcgttcgctt tggtgaacag atgatcgtta aagcgatgag tattctggta
    10741 ttcccgtttg ttggcgtact gatgctgctg gctctgtacc tgatcccgca gtggaacggc
    10801 gcagcactgg aaacgctgtc tctggacact gcatctgcaa ccggaaacgg tctgtggatg
    10861 accctgtggc tggcaattcc ggtaatggtg ttctcgttca accactctcc gatcatctct
    10921 tctttcgccg ttgcgaagcg tgaagagtac ggcgatatgg cagaacagaa atgctcgaag
    10981 atcctggcat tcgcacacat catgatggtg ctgaccgtaa tgttcttcgt cttcagctgc
    11041 gtactgagcc tgactccggc agacctggct gcggctaaag agcagaacat ctcgattctg
    11101 tcttacctgg ctaaccactt taacgcaccg gttatcgcgt ggatggctcc gattatcgcg
    11161 attatcgcta tcaccaaatc cttcctcggc cactacctgg gcgcacgtga aggcttcaac
    11221 ggtatggtga ttaaatctct gcgtggtaaa ggtaagtcta tcgaaatcaa caagctgaac
    11281 cgtatcactg cgctgttcat gctggtaacg acctggattg ttgccaccct gaacccgagc
    11341 atcctgggta tgattgaaac cctgggcggc ccaatcatcg cgatgatcct gttcctgatg
    11401 ccgatgtacg caattcagaa agtaccggca atgcgtaagt acagcggtca catcagcaac
    11461 gtattcgttg tcgtgatggg tctgattgca atctccgcaa tcttctactc tctgttcagc
    11521 taagtccttt cgcgccgctt tcgggcggcg cttcctccgt tttaacgcga tgtatttcct
    11581 atgattagcg tattcgatat tttcaaaatc ggcattggcc cttccagttc tcataccgtt
    11641 ggaccaatga aagcgggtaa acaatttacc gacgatctga ttgcccgtaa cctgcttaaa
    11701 gacgtgaccc gcgtggtggt tgacgtgtac ggctcgctct ctctgactgg taaaggccac
    11761 cacactgata tcgccattat tatgggcctg gcgggtaacc tgccggatac cgtggatatc
    11821 gattccatcc ctggttttat tcaggatgtg aatactcatg gtcgcctgat gctggcaaac
    11881 ggtcagcatg aagtggagtt cccggttgat cagtgcatga acttccatgc cgacaacctt
    11941 tctctgcatg aaaacggtat gcgcattacc gcgctggcgg gtgataaagt cgtttacagt
    12001 cagacttact actctattgg cggtggcttt atcgttgatg aagagcattt tggccagcag
    12061 gatagcgcac cggttgaagt tccttatccg tacagttcag cagccgatct gcaaaaacat
    12121 tgtcaggaaa ccgggctgtc actctctggc ctgatgatga aaaacgagct ggcgctgcac
    12181 agcaaagaag agctggaaca gcacctggcg aacgtctggg aagtgatgtg tggcggtatt
    12241 gagcgcggta tttccaccga aggtgtgtta cctggcaaac tgcgtgttcc acgccgtgct
    12301 gcggcactac gccggatgct ggtcagccag gataaaacca ccactgaccc gatggcggtt
    12361 gttgactgga tcaacatgtt tgcactggca gtgaacgaag agaacgctgc tggcggtcgc
    12421 gtggtgactg cgccgactaa cggtgcgtgc gggattatcc cggcagtgct ggcgtactac
    12481 gataagttta tccgcgaagt gaacgctaac tcactggctc gttacctgct ggtagccagc
    12541 gccattggtt ctctttataa gatgaacgcg tcgatttctg gtgccgaagt gggttgccag
    12601 ggtgaagttg gtgtggcgtg ctcaatggcg gcggctggtc tggcagaact gttaggtgca
    12661 agcccggcgc aggtgtgcat cgcggcggaa atcgccatgg agcacaacct cggtctgacg
    12721 tgtgacccgg tcgccggaca ggtacaggtg ccatgcatcg agcgtaacgc cattgcggca
    12781 gtaaaagcgg tgaacgccgc acgtatggcg ctgcgccgta ccagcgagcc gcgcgtctgc
    12841 ctcgataaag ttatcgaaac catgtacgaa acaggtaaag atatgaacgc caagtaccgc
    12901 gaaacctctc gcggcggcct ggcaatgaag atcgttgcct gcgattaatc gctctccaga
    12961 ggcctcgttt tgcgaggcct cttcccgatt tctcatccag ccatagcctg ttccagtatc
    13021 gaatgttacc cttatcgcct gatctttaag ggggttatcg tggctgttca tttgcttatt
    13081 gtcgatgcac tgaatcttat tcgtcgcatt catgccgttc aggggtcgcc ctgtgtcgaa
    13141 acctgccagc atgcgctcga tcagctcatt atgcacagcc agccaaccca cgcggtcgcc
    13201 gtttttgacg atgaaaaccg cagtagcggc tggcgtcatc agcgtttacc agattacaaa
    13261 gcgggtcgac cgccaatgcc ggaagagttg cacgacgaga tgcctgcatt acgcgccgcc
    13321 tttgagcaac gcggcgtccc gtgctggtca accagcggca acgaagccga tgacttagcc
    13381 gccacgctgg cggtcaaagt gacacaggcc gggcatcagg caacgattgt ttcgacagat
    13441 aaaggctact gtcagttact ttcaccgaca ttacgtattc gtgattactt ccagaaacgt
    13501 tggctggatg cgccatttat cgataaagaa tttggcgttc aaccgcagca gttgcccgat
    13561 tactggggac ttgcggggat cagcagttca aaggtaccgg gtgttgcggg aatcggacca
    13621 aaaagcgcca cgcagctgct ggtcgagttt cagagtctgg aagggatata tgagaatctg
    13681 gatgcggttg ccgaaaagtg gcgcaaaaaa ttagaaaccc ataaagagat ggcgtttctg
    13741 tgccgcgata ttgcccgctt acaaaccgat ttgcatatcg acggcaattt acagcaattg
    13801 cggttggtac ggtaacggcg tgccggatac gccgcaaacg tcgcatccgg cattatcaca
    13861 tcagcgcatt taccaggcgg tatggtaaag ctctacaata tcctcaagcg ttgcttcacg
    13921 cgggttgcca ccggtacaaa catcatccag tgccgcctgc gccagtgccg gaatgtcttc
    13981 cttgcgtaca ccaacatcac gcaaatgtgg cggaataccg acatcacggt tgagagcaaa
    14041 caccgcttca acagcggcat tacgcgcctc ttccaggctc ataccttcca ctttcacgcc
    14101 cataacgcgc gcgatatcgc ggtacttctc accggtaaag tcagcgttat aacgcatgac
    14161 atgcggtaac aggatggcgt ttgcaacacc gtgtggagtg ttataaaacg cgcccagtgg
    14221 atgcgccata ccatgcacca accctaaccc cacattcgag aagcccatac ccgcaacata
    14281 ctgcccgagc gccatttctt ctccggcatc cttatcacca gcaaccgatc ctcgcagcgc
    14341 cccagcaatg atttcaatcg ctttaatgtg cagtgcatcg gttagcgccc acgcgccacg
    14401 ggtaatatac ccctcaatag catgagtgag cgcatcgaca cccgtcgcag ctttcagcgc
    14461 tggaggcata ccatccatca tgtcagcgtc aataaacgcc acctgcggga tatcatgcgg
    14521 atcaacgcaa acaaacttgc gccgtttttc ttcgtcagtg atcacgtagt taatggtcac
    14581 ttctgccgca gtgcctgctg tggtggggat tgccagaatc ggtacactgg gtttattggt
    14641 cggggaaagt ccttccaggc tacgcacatc ggcaaactcc gggttgttgc tgataatgcc
    14701 aatcgcttta caagtatcct gtggagaacc accaccaata gcgatcaggt aatccgcgcc
    14761 gctattctgg aatacaccga gcccttcttt gacgacagta attgttgggt tgggcactac
    14821 gccgtcgtaa atcgcccatg ccagccctgc agcatccatc ttatcggtca ctttcgccac
    14881 cacgccgcat tgcaccagcg ttttatcggt gacgatcagc gccttctgat aaccacggcg
    14941 tttcacctca tcggttaaag ccccaacagc accccgacca aaccatgccg tttcgttcag
    15001 aatcattctg ttagccatca tccttctcct tgttgcttta cgaaattact cttcaatccg
    15061 taacccatag gttttgaatt tctccagcac tacggcaatc tcttcatcgc tcagcactgg
    15121 caccgggtcc gtaatcgcca gggtcgtcag gtaaagttgc gccagcactt caacttcatg
    15181 cgccagccat aacgcttttt ccagattcac ctcacaagcg ataagcccat gatgttgtaa
    15241 caaagttgcc ttacgatttt tgagagccag cgcaacatgt tcagaaagtt cgcgtgttcc
    15301 aaaggtcgca taaggcgcgc aaggaataga attaccgcca gccgccgcaa tcatgtagtg
    15361 aatagcgggg atcggtcggt taagaatgga aactgctgtg caatgaacgg catgattgtg
    15421 aacaaccgcg ttggcatccg gtctgctttg ataggccgcc atatggaaac gccactcgct
    15481 tgaggggagc tttccttcct catgtttacc gttgccatca ataaagacaa tatgcgactc
    15541 cgtcagtttt tcatatggaa tgcctgtagg cgtaatcagc atcccatcct gataacgtac
    15601 actgacgttc cccgctgtcc cctggttcag tcccaggcgg gtcatttcca ggcaagtttc
    15661 aataatctga cgagcaagtt tatttcgttc cattagctac ctctctctga ttcaaaacag
    15721 ggcaataatg ttgttccttt cacactattg aattagccgt ttaattaccc accatcttct
    15781 tcctgattaa caagaaagaa attcacaagc ttatattttg tgacctagtt caactaatca
    15841 cagtaaataa ctgcaagttc tctttttata accccattaa aaatgaccgc tcttaaaaat
    15901 atttatcaaa acggtcattt ttcta
//
LOCUS       DABSYT020000102        15561 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.102, whole genome shotgun sequence.
ACCESSION   DABSYT020000102 DABSYT020000000
VERSION     DABSYT020000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.102"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            125..328
                     /locus_tag="ID725_RS19240"
                     /old_locus_tag="pgaptmp_003848"
     CDS             125..328
                     /locus_tag="ID725_RS19240"
                     /old_locus_tag="pgaptmp_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGV7852348.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1407
                     /locus_tag="ID725_RS19245"
                     /old_locus_tag="pgaptmp_003849"
     CDS             328..1407
                     /locus_tag="ID725_RS19245"
                     /old_locus_tag="pgaptmp_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7852349.1"
                     /translation="MGRRRKNPEHEKLPPKVYPNKYSVWKPTSRESVTLTAIEDGLAA
                     LWKKYEETVNHRDRAMTFGRLWEKFLASAYYSELSPRTQKDYLQHQKKLLAVFGKVLA
                     DSVKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAKN
                     RERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGKT
                     GKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNHAGKISG
                     ILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPENI
                     PRKYSKKIFQVIPSVIFVTDLMMCK"
     gene            complement(1416..1505)
                     /locus_tag="ID725_RS19250"
                     /old_locus_tag="pgaptmp_003850"
     tRNA            complement(1416..1505)
                     /locus_tag="ID725_RS19250"
                     /old_locus_tag="pgaptmp_003850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1469..1471),aa:Ser,seq:cga)
     gene            1599..2396
                     /gene="mtfA"
                     /locus_tag="ID725_RS19255"
                     /old_locus_tag="pgaptmp_003851"
     CDS             1599..2396
                     /gene="mtfA"
                     /locus_tag="ID725_RS19255"
                     /old_locus_tag="pgaptmp_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGV7852350.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2497..2572
                     /locus_tag="ID725_RS19260"
                     /old_locus_tag="pgaptmp_003852"
     tRNA            2497..2572
                     /locus_tag="ID725_RS19260"
                     /old_locus_tag="pgaptmp_003852"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2530..2532,aa:Asn,seq:gtt)
     gene            2886..10868
                     /locus_tag="ID725_RS19265"
                     /old_locus_tag="pgaptmp_003853"
     CDS             2886..10868
                     /locus_tag="ID725_RS19265"
                     /old_locus_tag="pgaptmp_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGV7852351.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQIVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATV
                     ADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSKWER
                     ITATPKTGGPSYIYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFHSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(11130..12182)
                     /locus_tag="ID725_RS19270"
                     /old_locus_tag="pgaptmp_003854"
     CDS             complement(11130..12182)
                     /locus_tag="ID725_RS19270"
                     /old_locus_tag="pgaptmp_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGV7852352.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            12496..13812
                     /gene="shiA"
                     /locus_tag="ID725_RS19275"
                     /old_locus_tag="pgaptmp_003855"
     CDS             12496..13812
                     /gene="shiA"
                     /locus_tag="ID725_RS19275"
                     /old_locus_tag="pgaptmp_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGV7852353.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            13914..15368
                     /gene="amn"
                     /locus_tag="ID725_RS19280"
                     /old_locus_tag="pgaptmp_003856"
     CDS             13914..15368
                     /gene="amn"
                     /locus_tag="ID725_RS19280"
                     /old_locus_tag="pgaptmp_003856"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGV7852354.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4272 a         3699 c         3721 g         3869 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagaaa atattccaag taattccaag tgtgattttt
     1381 gtcactgact taatgatgtg taagtgattg aattttggcg gagagagggg gatttgaacc
     1441 cccggtggag ttgcccccac tccggttttc gagaccggtc cgttcagccg ctccggcatc
     1501 tctccgttca gatggttgcc atgatgccag gaaatttggc attttaacag tccctgtccg
     1561 tgcaattttg ttcaagtgac gagtttgcga gcaaaacgat gattaagtgg ccctggaaag
     1621 tacaagaatc agcacatcaa actgcccttc cctggcagga agcactatcg atcccccttt
     1681 taacgtgtct gacggaacag gaacaaagca aattagtcac tcttgccgaa cgttttttac
     1741 agcaaaagcg gcttgttcct ttacagggct ttgaactgga ttcattaaga agctgccgga
     1801 tagcacttct attttgccta cccgttctgg agttaggact ggaatggctg gatggttttc
     1861 atgaagtctt aatttatcct gcgccatttg tggtcgatga tgaatgggaa gacgatatcg
     1921 gtctggtgca taaccaacgt attgttcagt caggtcagag ctggcagcaa gggcctatcg
     1981 ttttgaactg gttggatata caagattctt ttgatgcttc tggttttaac ctgattattc
     2041 atgaagtcgc tcataagctg gacacccgta acggcgatcg cgccagcgga gttcccttta
     2101 ttccgttgcg tgaggttgct ggctgggaac acgatcttca tgctgcaatg aacaacattc
     2161 aggaagaaat cgaattggtt ggcgagaatg cggcgagcat tgatgcttat gctgccagtg
     2221 atcctgctga atgttttgcc gtactttctg aatatttctt tagcgcccca gaactttttg
     2281 ctcctcgttt cccttcattg tggcaacgtt tctgccaatt ttatcaacaa gatcctttgc
     2341 agagactgca tcacgctaat gatacagact cgttttcggc gacgaatgtt cattaattaa
     2401 caactttgca gattaattaa ccaattgaaa tgacttatga aatttagtgt tgacagacaa
     2461 ggtaccgcta agtaatatgc gccccgttca cacgattcct ctgtagttca gtcggtagaa
     2521 cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattctaa
     2581 aaattcgctt ttttagcgca atgtcactga ccttagttga acattgtttt ttaacggata
     2641 gcgggttttt aacatcttaa gcgccctcga cctttatggt tgagggcgtt ttgctatgaa
     2701 cgccatcacc attttcccct cgattataaa acttgagtta ttcagtagtc tcccctcttg
     2761 caactcacat ccaaaactgc ctaacgaaaa gttattaatt ttcaatcata ttgctatcag
     2821 gatttacatt ttttcgctgt gctagaaagg gcgcatttat gttagctcgt tcagggaagg
     2881 taagcatggc tacgaagaag agaagtggag aagaaataaa tgaccgacaa atcttatgcg
     2941 ggatgggaat taaactacgc cgcttaactg cgggtatctg cctgataact caacttgtgt
     3001 tccctatggc tgcggcagca caaggtgtgg taaacgccgc aacccaacaa ccagttcctg
     3061 cacaaattgc cattgcaaat gccaatacgg tgccctacac ccttggagcg ctggaatcgg
     3121 cccaaatcgt tgccgaacgt ttcggtattt cggtggctga gttacgcaaa ctcaaccagt
     3181 ttcgtacgtt tgctcgaggt tttgataatg tccgccaggg tgatgaactg gatgtcccgg
     3241 cacaagttag tgaaaataat ttaaccccgc caccgggtaa tagcagtggc aaccttgagc
     3301 aacagatagc cagtacttca cagcaaatcg ggtctctgct cgccgaggat atgaacagcg
     3361 agcaagcggc aaatatggcg cgtggatggg cctcttctca ggcttcaggc gcaatgacag
     3421 actggttaag ccgcttcggt accgcaagaa tcacgctggg cgtggatgaa gattttagcc
     3481 tgaagaactc ccagttcgat tttctccatc cgtggtatga aacgcctgat aatctctttt
     3541 tcagtcagca tactctccat cgtactgacg agcgtacgca gattaacaac ggcttgggtt
     3601 ggcgtcattt cactcccaca tggatgtcgg gcatcaactt ctttttcgac cacgatctta
     3661 gccgttacca ctcccgcgcc ggcattggcg cggagtactg gcgcgactat ctaaaattaa
     3721 gcagtaacgg ctatttgcga ctgaccaact ggcgcagcgc acctgaactg gacaacgatt
     3781 atgaagcacg cccggccaat ggctgggatg tacgcgcaga aggctggcta cccgcctggc
     3841 cgcaccttgg cggtaaactg gtctatgaac agtattatgg cgatgaagtg gccctgttcg
     3901 ataaagatga tcggcaaagt aatcctcatg ccataaccgc tggacttaac tataccccct
     3961 tcccgctgat gaccttcagc gcggagcaac gccagggtaa acagggcgaa aatgacaccc
     4021 gttttgccgt cgattttacc tggcaacctg gaagcgcgat gcagaaacag cttgacccga
     4081 atgaagtcga tgcacggcgt agccttgcag gcagccgttt tgatctggtg gatcgcaaca
     4141 acaacatcgt tctggaatat cgcaaaaaag aactggttcg cctgaccctg acagaccccg
     4201 tgacagggaa gtcaggagaa gtgaaatcac tggtttcgtc gctacaaacc aaatatgccc
     4261 tgaaaggcta taacgtcgaa gccaccgctc tggaagctgc cggtggtaaa gtggttacaa
     4321 cgggtaaaga tattctggtt accctgccgg cgtaccggtt caccagtacg ccagaaaccg
     4381 ataacacctg gccgattgaa gtcaccgctg aagatgtcaa aggcaatttt tcgaatcgtg
     4441 aacagagcat ggtagtcgtt caggctccta cgctaagcca gaaagattcc tcggtatcgt
     4501 taagtagcca gacgttgagc gcggattccc attcaaccgc cacactgact tttattgcgc
     4561 atgatgcagc aggtaatcct gttatcgggc tggtgctctc gacgcgtcac gaaggtgttc
     4621 aggacatcac cctttctgac tggaaagata atggtgacgg aagctatacc cagatcctga
     4681 ccacaggagc gatgtctggc acgctgacgc tgatgccaca gctgaacggt gtggatgcgg
     4741 ctaaagcccc cgccgtggtg aatatcattt ctgtttcgtc atcccggact cactcgtcaa
     4801 ttaagattga taaggaccgt tatctctccg ggaatcctat cgaggtgacg gtagaactga
     4861 gagatgaaaa tgacaaacct gttaaggagc aaaaacagca actgaatacc gcagtcagca
     4921 tcgacaacgt gaaacctggt gtcactacag actggaaaga aaccgcagat ggcgtctata
     4981 aggcaaccta taccgcctat accaaaggca gtgggcttac tgcgaagctg ttaatgcaaa
     5041 actggaatga agatttgcat accgctggat ttatcatcga cgccaacccg cagtcagcga
     5101 aaattgcgac attatctgcc agcaataatg gtgtgctcgc caatgagaat gcagcaaaca
     5161 ccgtctcggt caatgtcgct gatgaaggaa gcaacccaat caatgatcat accgtcacgt
     5221 ttgcggtatt aagcggatcg gcaacttcct ttaacaatca aaacaccgca aaaacggatg
     5281 ttaatggtct ggcgactttt gatctgaaaa gtagtaagca ggaagacaac acggttgaag
     5341 tcacccttga aaatggcgtg aaacaaacgt taatcgtcag ttttgtcggc gactcgagta
     5401 ccgcgcaggt tgatctgcag aagtcgaaaa atgaagtggt cgctgacggc aatgacagtg
     5461 ccacaatgac cgcgacagtt cgggatgcaa aaggcaacct gctcaatgac gtcaaggtca
     5521 ccttcaatgt caattcagca gcagcgaaac tgagccaaac cgaagtgaat agccacgacg
     5581 ggatcgccac agctacgctg accagtttga aaaatggtga ttatacggtt acggcctctg
     5641 tgagctctgg ttctcaggct aatcaacagg tgatttttat cggtgatcaa agtactgctg
     5701 ccctgaccct cagtgtgcct tcaggtgata tcaccgtcac caacacagct ccgctacata
     5761 tgactgcaac cttgcaggat aaaaatggca atccactaaa agataaagaa atcaccttct
     5821 ctgtgccaaa cgacgtcgca agtcggttct cgattagcaa cagcggaaaa ggcatgacgg
     5881 atagcaacgg gactgcaatc gcctccctga ccggcacgtt agcgggcacg catatgatca
     5941 cggctcgtct ggctaacagc aatgtcagcg atacacagcc aatgacgttt gtggcggata
     6001 aagacagagc ggttgtcgtt ctgcaaacat cgaaagcgga aatcattggg aatggcgtgg
     6061 atgagacgac tctgacagca acagttaaag atccttttga taacgtggtt aaaaatcttt
     6121 cagtagtctt ccgcacctcc cccgcagaca cgcaactgag tctgaacgcg cgtaatacta
     6181 atgagaacgg tattgccgaa gttaccctta agggcacggt tttgggtgtt catacagccg
     6241 aagccatact gcttaacggc aacagagata cgaaaatcgt caatattgcg cccgatgcca
     6301 gcaacgcgca ggtcaccctg aacatccctg cacaacaggt ggtgacgaat aacagtgaca
     6361 gcgtgcagct gacggcgacg gtgaaagacc cgtcgaatca tccggtggcg ggaataacgg
     6421 tgaacttcac catgccacag gacgtggcgg caaactttac ccttgaaaat aacggtattg
     6481 ccatcactca ggccaatggc gaagcgcatg tcaccctcaa aggcaaaaaa gcgggcacgc
     6541 atactgtgac cgccacgctg ggtaacaata atgccagcga tgcgcaacca gtcaccttcg
     6601 tggcggataa ggacagcgcg gttgtcgttc tgcaaacatc gaaagcggaa atcattggga
     6661 atggcgtgga tgagacgact ctgacggcaa cagtgaaaga tccttttgat aacgcagtaa
     6721 aagatctaca ggtcaccttc agtaccaacc ccgcagatac tcaacttagt cagagcaaaa
     6781 gcaatactaa cgacagtggt gtggccgaag ttacctttaa gggcacggtt ttgggtgttc
     6841 atacagccga agccacactg cctaacggca acaacgatac gaagatagtc aatattgcgc
     6901 ccgatgccag caacgcgcag gttacgctga acatccctgc tcaacaggtg gtgacgaata
     6961 acagcgacag cgtgcagctg acggcgacgg tgaaagatcc gtcgaatcat ccggtggcgg
     7021 gaataacggt gaacttcacc atgccacagg acgtggcggc aaactttacc ctcgaaaata
     7081 acggtattgc catcacccag gccaatgggg aagcgcatgt cacgctcaaa ggtaaaaaag
     7141 cgggtacgca tacggttacc gcaacgctga gtaataacaa taccagtgat tcacagccgg
     7201 taacgtttgt ggcggacaaa acctcggctc tggttgttct tcagatatca aaaaatgaga
     7261 tcacaggtaa tggcgtcgat agcgcaacgc taactgcaac ggtcaaagat cagttcgaca
     7321 atgaggtgaa caatcttccg gtaacattca gcacagcttc ttcaggcctc accctgaccc
     7381 caggggaaag taataccaat gagtctggca tcgcgcaggc cactctcgca ggcgttgcct
     7441 ttggtgagca gacggtcact gcatcactgg ctaataatgg tgccagcgac aacaaaactg
     7501 tgcattttat tggcgacaca gcggcggcaa aaattatcga gttgacgcct gtcccagaca
     7561 gcataatcgc aggtaccccg cagaacagct ccggcagcgt catcaccgcc acagtcgttg
     7621 ataataatgg ctttccggtg aaaggtgtga ctgtgaactt caccagcaac gcagcgacag
     7681 ccgaaatgac gaatggcggt caagccgtga cgaacgaaca gggtaaggct accgtcactt
     7741 ataccaatac ccgctcctcg atagaatcag gagcgagacc ggataccgtt gaggccagtc
     7801 tggaaaatgg tagctccacg cttagcacat caattaatgt caacgctgat gcgtctacgg
     7861 cacatctcac cttgctacag gcactttttg atacagtctc cgcaggcgac actaccaatc
     7921 tgtatattga ggtgaaggat aattacggca acggagtacc ccagcaggag gtaaccctca
     7981 gcgtttcacc aagtgaaggt gtgaccccca gtaataacgc tatatatacg accaatcacg
     8041 acggcaattt ttacgcaagc tttaccgcta caaaagccgg ggtataccaa gtgacggcaa
     8101 ccctcgaaaa tggcgattcg atgcaacaaa cagtgaccta tgtgccgaac gtagcgaatg
     8161 ctgaaatctc gctggcagcc tcgaaggatc cggtaattgc caacaataac gatctcacga
     8221 cactaacagc aacagtcgct gatacagagg gcaatgcgat agccaacagt gaggtaacat
     8281 ttactctgcc ggaagatgtg agggcgaact tcacgctggg cgatggcggt aaagtggtta
     8341 ctgatactga aggcaaagcg aaagtcacgc tgaaaggtac aaaagcaggc gctcatactg
     8401 ttacagcatc gatggctggc ggtaagagtg agcagttggt ggtgaacttt attgcggata
     8461 cactcactgc gcaggttaat cttaacgtta ccgaggacaa ttttatcgct aataacgtcg
     8521 ggatgaccag gctgcaggca acagtgactg atggaaacgg caacccgtta gccaatgagg
     8581 cggtgacatt cacgctaccg gcagatgtga gcgcaagctt tactctcgga caaggcggtt
     8641 ccgccattac tgacatcaac ggcaaggctg aagttacact gagcggtaca aaatccggca
     8701 cctaccccgt gacagttagc gtgaacaatt atggtgtcag tgatacgaaa caggtgactt
     8761 tgattgccga tgctggtacc gcaaaactag cctccttaac ctctgtatac tcattcgtcg
     8821 tcagcacgac cgagggcgcg accatgactg caagcgtcac tgacgctaac ggcaacccgg
     8881 tagaaggtat aaaagttaat ttccgcggaa cttccgtcac gctaagcagc accagcgttg
     8941 aaacggatga tcggggtttc gctgaaattc ttgtgacaag caccgaggtc ggactgaaaa
     9001 cagtttcagc ctctctggca gataaaccta ctgaagtcat ctcgcgatta ctgaatgcaa
     9061 aagcagatat taattctgca acgattacca gtctggagat acctgaaggt caggtcatgg
     9121 tcgcacaaga cgtagcagtt aaagctcacg tcaacgacca gtttggcaat ccgattctta
     9181 atgaatctgt aacattcagt gcagaaccac cagagcacat gaccatcagc caaaatattg
     9241 tctctactga tacgcatggt atagccgagg tcactatgac gcccgaaaga aacggttcgt
     9301 atatggtgaa agcatccctg gcgaatggat cctcttatga gaaggatctg gtggtaatcg
     9361 atcaaaaact gacactctcg gcgtccagcc cgcttatcgg tgtcaattcc ccaacaggtg
     9421 caactctgac ggcaacgcta acttctgcaa atggcactcc agtggagggt caggtcatca
     9481 actttagcgt aacgccagaa ggtgcgacgt taagtggcgg aaaagtgaga accaactctt
     9541 caggtcaggc tccagtcgtt ctgaccagca ataaagtcgg tacatatacg gtgactgcat
     9601 cgttccataa cggcgtaaca atacagacac agacaatcgt gaaagtcact ggcaactcaa
     9661 gcaccgccca tgttgctagc tttatcgctg atccatcgac tatagccgcc accaacagtg
     9721 atttaagtac cttaaaggca acggttgagg atggcagtgg taacctgatc gaaggtctca
     9781 ctgtgtactt cgccttaaaa agcggctctg ccacattaac gtcattaaca gcggtgacag
     9841 atcaaaacgg aatcgcgaca acaagcgtga gaggagcgat aacggggagc gtcacggtaa
     9901 gcgcagtcac gaccgctggt ggaatgcaaa cagtagatat aacgctggtg gcaggcccgg
     9961 cagacgcctc gcagtccgtc cttaagaaca atcggtcatc attgaaagga gactttaccg
    10021 atagtgctga gctacatctt gttctgcacg atatatcagg caatccgatc aaagtttctg
    10081 aagggctgga atttgtgcag tcaggtacca acgcgcccta tgtgcaagtt agtgcaattg
    10141 actacagtaa aaatttctca ggcgagtaca aagccactgt tacaggcggc ggagagggta
    10201 tcgcaacgct gatccctgta ttgaatggtg ttcatcaagc gggtctgagt accacaatac
    10261 aattcactcg cgcagaagac aaaataatga gcggtacagt gttagtcaat ggtgctaacc
    10321 taccgacaac tacattccct tcgcaggggt tcactggggc gtattatcag ttgaataatg
    10381 acaactttgc cccaggaaaa acggcggctg attatgagtt ttcaagctct gcctcctggg
    10441 ttgatgttga tgctaccggt aaagtgacat ttaaaaatgt cggcagcaaa tgggagagga
    10501 ttacggcgac gccaaaaaca ggcggcccta gctatatata cgaaatccga gtgaagagtt
    10561 ggtgggtgaa cgccggcgat gctttcatga tatacagcct tgctgaaaat ttttgcagta
    10621 gcaatggcta cacacttccc cttggagacc atttaaacca tagtcgttcc cgaggcatcg
    10681 ggtcactgta cagtgaatgg ggagatatgg ggcattacac gactgaagct ggttttcatt
    10741 caaatatgta ttggtcatcg agtcccgcaa actcaaacga acaatacgta gtttccctgg
    10801 caacaggtga tcaaagcgta tttgaaaagc ttgggtttgc ttatgcgaca tgttataaaa
    10861 acctctgatt ttccgttata atttcttaaa gagtgctcca ttcggggcac tcttttttac
    10921 attccctcta tagagcttgc ccttctctca cagaaaatga ataaataaaa atgcgatacc
    10981 gcaagaatcg cgttcgatgt gactatgatc gtatccggtg agtacagctt ttatccaaag
    11041 gctggttttc tgtgatgatt gaccagcctc agagtattga tgacaccacc gttcataaca
    11101 gatcggcatt actatgcata agtactgtct catattctcg ccaaaacact ttttaatgcc
    11161 tttgcgaatt catccagact tatattgtca attttttgcc caacgacgac tgactccgaa
    11221 acatcggcca ttgcgggcca tcgcgttttg taatcagcat aaattgcaac aacgggggta
    11281 ttataggcac ttgccatatg taatattgag gtgtcaggag ttattgcgat ataagcatct
    11341 tttatgattg ctgctgaacg tttaatcgag ggtgataagg ataaacggta gacattatta
    11401 taacaatcca ctaataccct ggctttatct tccccttttg gtccatgaac gatcactatt
    11461 ggtatatctg tttcactttg aattttttct attagatttt ctgcaatcga tagtgaaaat
    11521 gtacgatcct gcgagctacc ttctaaatta agcgcaatgt aagatcctaa cgatcgcatt
    11581 tcctcgcgca cctcatcgag tacatcctca cttagtggca attcatatat tggcctgact
    11641 gctggaaaac ccgcctcacg catcataaat gcccatgtca tgggtacggg agcccggagt
    11701 ttctgatcca tccgggacgc gttcttacac acaggggaat agcacttcat ggttaaacca
    11761 acaacctgaa aattcgtttt tgctttcaac tgactgataa ataacatcgt tttcaggttc
    11821 tttttacgca tcgcctctat acaaagatcc ggcgtaccgt attgctgtgt tatgttcttc
    11881 gctaaatctt ttatttcttt taatgttgcg tgatcctgca tagtcattgt gactaatgtt
    11941 aatttagtct gttcaagttt gagcgcatta aagacttcta aattaattgt cgacgtaaca
    12001 attaatagat gcttaatttt atgcaattca agcgcccgaa taacaggaaa gatggccata
    12061 gcatcgccaa tctgatcggg aatatggatg acaacaaagt ctgttttttc aatattgaaa
    12121 ttataagctt tataatcgta gtaactaaat gcaatacgtc tcaacaatga tactaaaaac
    12181 ataactaacc tcgcctccct actggttata atgcaatgca gtctatcaga atcatcaggg
    12241 tgcaattttg tgcatatgcg gacttttatg tttcatatct cgaacctgtg gggcctctgc
    12301 ttaatcctta aacaacacca gcaactcctg cgctttcatc ttccagcgaa tttttcatgt
    12361 tgccgttaat cagccataaa atcatttgca gatgcgctct gtcgaggtag tctcataagg
    12421 ttcgtttata gatcgacgca atgtgagtta ccttttccat actaattata aaaagacagt
    12481 acaaacagga tcattatgga ctccacgctc atctccactc gtcccgatga aaggacggtt
    12541 tcgttaagtc gcgcccgacg agctgcgtta ggcagtttcg ctggtgccgt cgtcgactgg
    12601 tatgattttt tactctatgg cattaccgcc gcactggtgt ttaatcgcga gtttttcccg
    12661 caagtaagcc cggctatggg aacgctcgcc gcatttgcca cctttggcgt cggattcctt
    12721 ttccgtccgc tcggcggtgt cattttcggt cactttggcg accgactggg acgtaagcgc
    12781 atgttaatgc tgaccgtctg gatgatgggc atcgcgacag ccttgattgg tattcttcct
    12841 tcattctcga ccattgggtg gtgggcacct attttactgg tgacgctgcg tgccattcag
    12901 ggatttgccg tcggcggcga atggggaggc gcggcgttgc tttccgttga aagtgcaccg
    12961 aaaaataaaa aagcctttta cagtagcggt gtacaagttg gctacggtgt aggtttactg
    13021 ctttcaaccg gactggtttc attgatcagt atgatgacga ctgacgaaca gtttttaagc
    13081 tggggctggc gcattccttt cctgtttagc atcgtactgg tactgggagc attgtgggtg
    13141 cgcaatggca tggaagagtc cgcggaattt gaacaacagc aatataatca agcggccgcg
    13201 aaaaaacgca tcccggttat cgaagcgctg ttacgacatc ccggtgcttt cctgaagatt
    13261 attgcgctac gactgtgcga gttgctgaca atgtacatcg ttaccgcctt tgcacttaat
    13321 tattcaactc agaatatggg gttaccgcgc gaacttttcc ttaatattgg tttgctggta
    13381 ggtggattaa gctgcctgac aattccctgt tttgcctggc ttgccgatcg ttttggtcgc
    13441 cgcagggttt atatcacagg cgcgttgatc ggaacgttga gcgcatttcc tttctttatg
    13501 gcgcttgaag cacaatctat tttctggata gttttcttct ccataatgct ggcaaacatt
    13561 gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta ccgaaatgtt tggtgccagt
    13621 tatcgctata gtggtgctgg agtcggttat caggttgcca gtgtggttgg cggtggattt
    13681 acacctttta ttgccgctgc gctcatcact tactttgccg ggaactggca tagcgtcgcc
    13741 atttatttgc tggctggatg tctgatttcc gcaatgaccg ctttgttgat gaaagacaat
    13801 caacgcgctt gatagtctgg cgaagatcat ccgatcttcg ccttacactt ttgtttcaca
    13861 ttcctgtgac atactatcgg atgtgcggta attgtatgga acaggagaca cacatgaata
    13921 ataagggctc cggtctgacc ccagctcagg cactggataa actcgacgcg ctgtatgagc
    13981 aatctgtagt cgcattacgc aacgccattg gcaactatat tacaagtggc gaattacctg
    14041 atgaaaacgc ccgcaaacaa ggtctttttg tctatccatc tctgaccgta acctgggacg
    14101 gtagcacaac aaatcccccc aaaacgcgcg catttggtcg ttttactcac gcaggcagct
    14161 acaccaccac gattactcgc cctactctct ttcgttcgta tcttaatgaa caacttacgt
    14221 tgctgtatca ggattatggc gcgcatatct cggtgcaacc ctcacagcat gaaatccctt
    14281 atccttatgt catcgatggc tctgaattaa cacttgatcg ctcaatgagc gctgggttaa
    14341 ctcgctattt cccgacaaca gaactggcgc aaattggcga tgaaactgca gacggcattt
    14401 atcatccaac tgaattctcc ccgctatcgc attttgatgc gcgccgcgtc gatttttccc
    14461 tcgcgcggtt gcgccattat accggtacgc cagttgaaca ttttcagccg ttcgtcttgt
    14521 ttaccaacta cacacgttat gtggatgaat tcgttcgttg gggatgcagc cagatcctcg
    14581 atcctgatag tccctacatt gccctttctt gtgctggcgg gaactggatc accgccgaaa
    14641 ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa aaaacaccag atgccagcat
    14701 ggcatttaat taccgccgat ggtcagggta ttactctggt gaatattggc gtgggaccgt
    14761 caaatgctaa aaccatctgc gatcatctgg cagtgctacg cccggatgtc tggttgatga
    14821 ttggtcactg tggcggatta cgtgaaagtc aggccattgg cgattatgta cttgcacacg
    14881 cttatttacg cgatgaccac gttcttgatg cggttctgcc acccgatatt cctattccga
    14941 gcattgctga agtgcaacgt gcgctttatg acgccaccaa gctggtgagc ggcaggcccg
    15001 gtgaggaagt caaacagcgg ctacgtactg gtactgtggt aaccacagat gacaggaact
    15061 gggaattacg ttactcagct tctgcacttc gttttaactt aagccgggcc gtagcaattg
    15121 atatggaaag tgcaaccatt gccgcgcaag gatatcgttt ccgcgtgcca tacgggacac
    15181 tactgtgtgt ttcagataaa ccgttgcatg gcgagattaa acttcccggt caggctaacc
    15241 gtttttatga aggcgctatt tccgaacacc tacaaattgg cattcgggcg atcgatttgc
    15301 tgcgcgcaga aggcgaccga ctgcattcac gtaaattacg aacctttaat gagccgccgt
    15361 tccgataata agttctgaac cttcttatca aaaatagatg tgaaggatta agtaagacat
    15421 ctggcaaata gcccgcaata aacgcgggct gtttgcttcc ttgggcggat acgagtttta
    15481 ttatcgtctt aatgatttcc acatattaaa agcaagtatg ctttcaaaac acaattataa
    15541 aaaatcccgc caacaatata a
//
LOCUS       DABSYT020000103        15379 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.103, whole genome shotgun sequence.
ACCESSION   DABSYT020000103 DABSYT020000000
VERSION     DABSYT020000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.103"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(136..588)
                     /gene="yafP"
                     /locus_tag="ID725_RS19285"
                     /old_locus_tag="pgaptmp_003857"
     CDS             complement(136..588)
                     /gene="yafP"
                     /locus_tag="ID725_RS19285"
                     /old_locus_tag="pgaptmp_003857"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGV7852355.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(598..996)
                     /gene="yafO"
                     /locus_tag="ID725_RS19290"
                     /old_locus_tag="pgaptmp_003858"
     CDS             complement(598..996)
                     /gene="yafO"
                     /locus_tag="ID725_RS19290"
                     /old_locus_tag="pgaptmp_003858"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HGV7852356.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(999..1292)
                     /gene="yafN"
                     /locus_tag="ID725_RS19295"
                     /old_locus_tag="pgaptmp_003859"
     CDS             complement(999..1292)
                     /gene="yafN"
                     /locus_tag="ID725_RS19295"
                     /old_locus_tag="pgaptmp_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HGV7852357.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(1344..2399)
                     /gene="dinB"
                     /locus_tag="ID725_RS19300"
                     /old_locus_tag="pgaptmp_003860"
     CDS             complement(1344..2399)
                     /gene="dinB"
                     /locus_tag="ID725_RS19300"
                     /old_locus_tag="pgaptmp_003860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGV7852358.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2470..3255)
                     /gene="lafU"
                     /locus_tag="ID725_RS19305"
                     /old_locus_tag="pgaptmp_003861"
     CDS             complement(2470..3255)
                     /gene="lafU"
                     /locus_tag="ID725_RS19305"
                     /old_locus_tag="pgaptmp_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGV7852359.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3227..4939
                     /locus_tag="ID725_RS19310"
                     /old_locus_tag="pgaptmp_003862"
                     /pseudo
     CDS             3227..4939
                     /locus_tag="ID725_RS19310"
                     /old_locus_tag="pgaptmp_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(5084..5581)
                     /gene="rayT"
                     /locus_tag="ID725_RS19315"
                     /old_locus_tag="pgaptmp_003863"
                     /pseudo
     CDS             complement(5084..5581)
                     /gene="rayT"
                     /locus_tag="ID725_RS19315"
                     /old_locus_tag="pgaptmp_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(5757..6506)
                     /gene="yafL"
                     /locus_tag="ID725_RS19320"
                     /old_locus_tag="pgaptmp_003864"
     CDS             complement(5757..6506)
                     /gene="yafL"
                     /locus_tag="ID725_RS19320"
                     /old_locus_tag="pgaptmp_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGV7852360.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            6716..6976
                     /gene="dinJ"
                     /locus_tag="ID725_RS19325"
                     /old_locus_tag="pgaptmp_003865"
     CDS             6716..6976
                     /gene="dinJ"
                     /locus_tag="ID725_RS19325"
                     /old_locus_tag="pgaptmp_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGV7852361.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGV"
     gene            6979..7255
                     /gene="yafQ"
                     /locus_tag="ID725_RS19330"
                     /old_locus_tag="pgaptmp_003866"
                     /pseudo
     CDS             6979..7255
                     /gene="yafQ"
                     /locus_tag="ID725_RS19330"
                     /old_locus_tag="pgaptmp_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
     gene            7411..8151
                     /gene="dpaA"
                     /locus_tag="ID725_RS19335"
                     /old_locus_tag="pgaptmp_003867"
     CDS             7411..8151
                     /gene="dpaA"
                     /locus_tag="ID725_RS19335"
                     /old_locus_tag="pgaptmp_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGV7852362.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(8122..8889)
                     /gene="yafJ"
                     /locus_tag="ID725_RS19340"
                     /old_locus_tag="pgaptmp_003868"
     CDS             complement(8122..8889)
                     /gene="yafJ"
                     /locus_tag="ID725_RS19340"
                     /old_locus_tag="pgaptmp_003868"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGV7852363.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(8994..9572)
                     /gene="lpcA"
                     /locus_tag="ID725_RS19345"
                     /old_locus_tag="pgaptmp_003869"
     CDS             complement(8994..9572)
                     /gene="lpcA"
                     /locus_tag="ID725_RS19345"
                     /old_locus_tag="pgaptmp_003869"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGV7852364.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            9812..12256
                     /gene="fadE"
                     /locus_tag="ID725_RS19350"
                     /old_locus_tag="pgaptmp_003870"
     CDS             9812..12256
                     /gene="fadE"
                     /locus_tag="ID725_RS19350"
                     /old_locus_tag="pgaptmp_003870"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGV7852365.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(12299..12745)
                     /gene="ivy"
                     /locus_tag="ID725_RS19355"
                     /old_locus_tag="pgaptmp_003871"
     CDS             complement(12299..12745)
                     /gene="ivy"
                     /locus_tag="ID725_RS19355"
                     /old_locus_tag="pgaptmp_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGV7852366.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            12926..13696
                     /gene="yafV"
                     /locus_tag="ID725_RS19360"
                     /old_locus_tag="pgaptmp_003872"
     CDS             12926..13696
                     /gene="yafV"
                     /locus_tag="ID725_RS19360"
                     /old_locus_tag="pgaptmp_003872"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGV7852367.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(13814..14986)
                     /locus_tag="ID725_RS19365"
                     /old_locus_tag="pgaptmp_003873"
     CDS             complement(13814..14986)
                     /locus_tag="ID725_RS19365"
                     /old_locus_tag="pgaptmp_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HGV7852368.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLN
                     LLDLKKNLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPVNVFSNY
                     KGDSFSTQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQKKEDKSAE
                     GVSIRYYISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRGNAAEIISG
                     IKKMALNLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
     gene            15067..15252
                     /locus_tag="ID725_RS19370"
                     /old_locus_tag="pgaptmp_003874"
     CDS             15067..15252
                     /locus_tag="ID725_RS19370"
                     /old_locus_tag="pgaptmp_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852369.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHHNSLHN"
     gene            complement(15167..>15379)
                     /gene="ydcD"
                     /locus_tag="ID725_RS19375"
                     /old_locus_tag="pgaptmp_003875"
     CDS             complement(15167..>15379)
                     /gene="ydcD"
                     /locus_tag="ID725_RS19375"
                     /old_locus_tag="pgaptmp_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HGV7852370.1"
                     /translation="FFNDILYSVRLKNISKLENMEFCATKQRVYFSDKNKKASYKIIN
                     YGDYYDVDYYDNNLKNEVFDWIGKWS"
BASE COUNT         3745 a         3876 c         3803 g         3955 t
ORIGIN      
        1 aaaaaaagaa ttaatcctgg aaaggtctgc aaagagtcat tgataaagga tttgtcatga
       61 tttacctcct tgcagtttgt ggaataagaa aacgcgcaca taccaggcgg gcgttatttt
      121 cattgcaagc tggatttaat gttgcggttt atatcgcata gaaaaattag taaaccacgc
      181 tccccggcat tcaacgcgct gctgcttaac tgtctgaaaa ccataacgtt caaaaaaggg
      241 ttttgcggtt atgctggcgt ccaccgtaag ttcggattca gacttaatcc aaggttttaa
      301 caaagcgctg gcaaccccac ggcgggtgta ttcagggtca acaaataaca tatcgatata
      361 atgttcaatg cgggaaataa aaccaaccgg ttgtgcatta atgactgcaa cccgcacttg
      421 tgatttcgcg agtttctcct tccagcgaga ttcgtcaatc tgcgcccagg cggcaatttg
      481 ttgtggtgaa taatgctgac tggcggtcat cataaccgct ctaatgaaaa tagcgcatag
      541 ttgctgaaaa tcgccaggct gatagtttct tatttgtatg ttattcataa tataaattca
      601 aaaacgcatg cgaaacgctt ctgccatttt cccaatttta tgcatttggt tgttatctcg
      661 agccagttta tgaggttcag gtttcagaat ggcaatgagc aaccatgctt gctcatcaaa
      721 cgcaccctga caatatacca aatgcgattc gtcattcgtt ctgctgaatt ggcgcaactg
      781 tggcggaaat ggattattca catttgccag atgaatatga gcaactcgct caaatttgat
      841 taatggccaa gtaaacgagt cgtcgtagag tgcatcgcga ccaaatatat ctggcaaaac
      901 accgtcacgc ttataggaaa taaaatccgc cgttaacgca tcaagctcct ctgctgtaag
      961 ttgcaggcga ataagttttg ttttgaatac ccgcatcctt attccttaaa gtcattgaaa
     1021 tcatcatccg tcatatcatt aagatatttt tcagctcggc gtgtaatttc ctctaatctt
     1081 gcagcacttg gacggaagcg cgcctttatg ggctttttct cctcttgttc agcaagcatg
     1141 tccattaacg cttcgaatgc gctggcactt aagagatatc ctgcggggcg attattagaa
     1201 agaaccgcaa ccggttgatc aataaagtat ttagctgggt ttttacgtaa ttcagtgata
     1261 ttgaccgatt tttcagcgag aattcgatgc atacagtgat cccctcataa taatgcacac
     1321 cagaatatac ataatagtat acatcataat cccagcacca gttgtctttc catttgaggg
     1381 tcaagcaacg tcacatgcag ccccaccagt cgcacaccgc gcccgccgcg gcgttcatcc
     1441 caggttttac gcgcggtggc gattaagtca gctttattca gccgcggcca gacgtgctcc
     1501 tgggtggttt gctgaaaatc gtcgaacttt aatttcaccc cctggcgagc aatcagtaaa
     1561 tcaggtttta cctttgccag acggcgttca agttccggat acagccgctc gataatcgct
     1621 tcacattcag accagtgatg aatatcttcc gccatcgtgc gttccacgcc gacggatttt
     1681 cgtaaccgtt cgctgttaac gtcgcgctcg tcaatcccct gactacgctc ccacaaaatg
     1741 cggccaaatt tgccaaagcg tttaagcaga atcaccagat cacacttttg tacatcaccg
     1801 caggtccgca gccccatcgc ttccagcttt gccgctgaga ctttgccaac accgggaatt
     1861 tttgccagcg gtaaggtttg cagaaatgcc gaaacttctg ccggcgtaat cacaaactgg
     1921 ccgttgggtt tattcatgtc ggaggcgatt ttggcgagaa actttactgg tgccacgccc
     1981 gcagacgccg tcagttgcag ctcgctgaaa attgtctggc ggatttcctg ggcgatgagg
     2041 gtcgcagaac cgtggcaatg gacgctatca gtgacatcga ggtaggcttc atccagtgac
     2101 aacggttcaa tacgcgaggt gtagcgcgag aagatttcac ggatatgatt tgaggcttct
     2161 ttgtaggcgt caaagcgccc cggaagcaag gtgagatgtg ggcataattt gagcgccatc
     2221 cctgtcggca tagcgctgcg tacgccaaat ttacgcgcgg gataattggc ggtgctgatc
     2281 accccccgac gttcgcggct gccgccaata gcaataggga tatcgcgcag ggcgggattg
     2341 tcgcgcatct ccactgcggc gaaaaagcag tccatatcca catgaatgat tttacgcatt
     2401 actcacctct caatactggt aaagtataca gtgatttcag ggtttgagaa atgcgtaaag
     2461 attcagcatt tagcgcgttc gctgcgaaag cacctgctgc ttatccagct tttgcaccag
     2521 cggctgcacc actttatccc catgctgacc aaagtattga tacagcgtat cggacgcact
     2581 tttggtcagc accataatct caatgcgtcg gttgcccgcg ctttgcggat ttttcgcgtc
     2641 cagcagcatc tggtccgcca ttgcgcttac ctgcatcact ttatcttccg gcattccggc
     2701 ctcttccagc acccgacgag ctgaaagcgc gcgatcgccc gaaaggttcc agttgttgta
     2761 gatattgttt ttgtaggcca tcgcatcggt atgcccggta ataataattt tgttatcgag
     2821 cgagtcgaac actggtgcga gctccaccag caaagtttta aagaacggca taatctgcgc
     2881 gctgccgcgt tcgaacatat tgcgattctg gtcgtcttta atcagcacgc gtaatccctg
     2941 cgggacaatc tccatctcca gattcgcttc catatgcgca tcacgggcaa tggtgttgat
     3001 actggttgcc agctcgccaa gctcggtggc ggatttcttc ttcagcagcg ccgttttctc
     3061 attcacatca cgcgcttttt tctccgtctc ttccggcacc gccacggtgg caggttttgg
     3121 cggatgagac gggctgattt tatttaacgg cgacagccca ccaccgttaa aaatcgactg
     3181 cccgtgcagc gcggcgataa tgctttcacg ctcggattta ctgacgctgt tgacaatcaa
     3241 ctttatcgtc gtcaccaaag gggccgagcg tatttccgag gtttctgccc gctttaccct
     3301 ggatgcgatg cccggcaaac agatggcgat tgacgccgat cttaacgccg gattgatcaa
     3361 ccaggcacag gcgcaaaccc ggcgtaaaga tgttgccagc gaggccgatt tctacggcgc
     3421 gatggacggg gcatcgaagt ttgtgcgcgg ggatgccatc gccgggatga tgattctggc
     3481 gattaacctg atcggcggcg tctgtatcgg gatatttaaa tacaacctga gcgccgacgc
     3541 cgccttccag caatatgtgc tgatgaccat cggcgatggg ctggtggcgc agatcccttc
     3601 cctcctgctc tccaccgcag cggcgattat cgtcacccgc gtcagcgaca acggcgatat
     3661 cgcccatgac gtgcgccacc agctgctggc aagcccgtcg gtgctctaca ccgccaccgg
     3721 gattatgttt gtgctggcgg tggtgccggg aatgccgcac ctgccgtttt tgctgttcag
     3781 cgccctgctg ggttttaccg gctggcggat gagcaaacag ccgcaggcgg cggaggcgga
     3841 agagaaaagc ctcgaaacgc tgacccgcac catcactgaa accagcgagc aacaggtcag
     3901 ttgggaaacc attccgctta tcgagcctat cagtttaagc ctcggctaca agctggtggc
     3961 gctggtcgat aaagcgcagg gcaacccgct cacccagcgt attcgcggcg tacggcaggt
     4021 gatatccgac ggtaacggcg tgctgctgcc ggagatccgc attcgggaaa acttccgcct
     4081 caagcccagc cagtacgcca ttttcatcaa cggcattaag gccgatgaag cggatattcc
     4141 ggcggataaa ctgatggcgc tgccctccag cgaaacctac ggcgagattg acggcgtgct
     4201 ggggaacgac ccggcgtacg ggatgccggt gacctggatc cagcccgcgc agaaggcgaa
     4261 ggcgctgaat atggggtatc aggtgatcga cagcgccagc gtgattgcca cgcatgtgaa
     4321 caagattgtg cgcagctata ttcctgattt gtttaattat gatgacatca cgcagctgca
     4381 taaccgcctg tcgtcgatgg ccccgcgcct ggcggaagat ttgagcgcgg cgctgaatta
     4441 cagccagttg ctgaaagtgt accgtgcgct gctgaccgaa ggcgtttccc tgcgcgatat
     4501 cgtcaccatc gccaccgtgc tggtcgccag tagcgcggtg actaaagatc atattctgct
     4561 ggcggccgat gtgcgcctgg cgctgcggcg cagcattacc catccgttcg ttcgcaagca
     4621 ggagctgacg gtgtatacgc tgaataatga gctggaaaat ctgctgacta acgtagtgaa
     4681 tcaggcgcaa cagggcggta aagtgatgct cgacagcgtg ccagtggacc cgaatatgct
     4741 caaccagttc cagagcacga tgccgcaggt gaaagagcag atgaaagcgg cggggaaaga
     4801 cccggtgctg ctggtgccgc cgcagctgcg ccctttgctg gcgcgctatg caaggttgtt
     4861 tgcgccgggg ctgcatgtgc tgtcgtataa cgaagtgccg gatgagctgg agttgaagat
     4921 aatgggggcg ttgagttgat tggcctttaa ggggcgcatc cggcattggt gcacaattgc
     4981 cggatgcggc gtgaacgcct tatccggcct acggttgagt gcagttttgt aggcatgata
     5041 agacgcgcca gcgtcgcatc aggcatccgg caccagcgca caattatgaa atgatacgct
     5101 ccccggcact aaaatccgtt atgtctcccg cccaatcaat gggatataac cctcgcgcca
     5161 catcgcgatg gaacgttgag aatggccaat cactcacttg ctttacccag ccatgtttta
     5221 ctggatttat ataaatataa tcaacatgat ggcgatagtc tttggtattg cggatggtgt
     5281 gctcctaaaa acgtggttgc cagatatttt tcaatccaca agcatgggtg aactgctttt
     5341 taatctcccg ccagcgggag gaaaaatcat catcgctttc gggtaatgtc catatgcaat
     5401 gcatatgctc cggcaaaacg acccaggcgt taatttcaaa aggtctgtct cgcttaacgt
     5461 taataatggc gttacggagc atctggaact gagcggtcag aaggtggctt cgacgatttc
     5521 gtaggttaac cgtgaaaaac caggttcccc ctttgatgta ataccgacga tattcagaca
     5581 tggcaactcc tttttctcaa cggtaagaag aaaaggaaag gtcggcaatg aggtgatgga
     5641 ataacgggaa agcggctcgc aaaaacgcac agatttgtag gcatgataag acgcgtcagc
     5701 gtcgtatcag gcatctgcgc acaactgccg gatgcggcgt gaatgcctta tccgtcctac
     5761 aaaatcgtct cttccgtcaa aatccggcgc gcgcctaaaa agtgatcctg ccagaaaggt
     5821 tcagctaacc gacttacccg aatggtttcc ccggtgcgcg gtgactcaat aaactgaccg
     5881 tcgcccagat acacccccat atggtcggca atctcgcggc tgtggatatg gaaaaacagc
     5941 aaatctcccc ggcgcaggtc gttattcgcc acaatcgttg cccgatggta gtgatacatc
     6001 tcattagccg tacgcgggag cttcgcctcg aggatcttgt tgtaggcata aaaaaccaac
     6061 ccgctacagt caaagccttt atcaggccgc gtgccgcccc agacgtaagg cttgccaagc
     6121 tgctgctcca ggcggtgaat cgccacttcg gtaatgttat gcagatggtc gctgcttaaa
     6181 aagtggttgt tttccgccag cgcctgccag cgtctgtcac tcgccttgag cggtgcagga
     6241 aaccattctg gatgctgttt tatctgttcg cggttgtgct ggcgtagcgc ccttctgctt
     6301 tcggcattgc cttccacaat atagctgacc tgctttttca gatgggtttg gtactgtttt
     6361 aataaacgcg cacggttttg catccgctgg cgggcggcgt agctgaaaga gatatgtgat
     6421 gtggtggcgc tggcaaatga agaaaacggc aaacaaatca acagtaatcc cgacaataca
     6481 aaggatggaa tcgacggcaa ggacatactc aataatggct ggtcatgcat tgatgttaca
     6541 gtgtatcccg gcccgtacct gaacaaactt aataaaccat tattataaaa ttaataaata
     6601 tatttcagtt aattatgtaa aaacatccgt ttattaaatt actcatagat tgtcgctgtt
     6661 gctcatttga gctacaattc aagctgaata aatatacagc ataggagata tcccaatggc
     6721 tgctaacgca tttgttcgcg cccgaatcga tgaagatctg aagaatcagg cggcggacgt
     6781 actggccggg atggggctga ccatctctga cctggttcgc ataaccctca caaaggtcgc
     6841 gcgtgaaaag gcattgccgt ttgatttacg cgagcctaat caattaacca ttcaatcaat
     6901 caaaaacagc gaagctggcg ttgatgttca taaggccaaa gacgccgatg atttatttga
     6961 taaattagga gtttaaatat gattcaaagg gatattgaac tcgggacaat tttcaaagga
     7021 tgtaaaactt gcacaaaagc gtcataagga tatgaataaa ttgaaatatc ttatgacgct
     7081 tcttatcaat aatactttac cgcttccagc tgtttataaa gaccacccgc tgcaaggttc
     7141 atggaaaggt tatcgcgatg ctcatgtcga accggactgg atcctgattt acaaacttac
     7201 cgataaactt ttacgatttg agagaactgg aactcacgcg gcgctctttg ggtaaagccc
     7261 gctgccgatg aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc ttttggtgat
     7321 aatgtgagcc ttcccaatcc agactattcc gatttgggat ccattttgcc gtcctggcgt
     7381 gtgtaaccgt tttatcaagg aataagcagt atgcgtaaaa tcgcattaat tcttgcgatg
     7441 ttgttgatcc cgtgcgtttc gtttgccggt ttgctgggca gcagtagctc gaccacgcca
     7501 gtgagcaaag agtataagca gcagttgatg ggatcccctg tctacatcca gatcttcaag
     7561 gaagaacgta cgctcgatct ctacgtcaaa atgggcgagc aatatcaact gctcgacagc
     7621 tataaaatct gtaaatattc cggcggctta gggccaaaac agcgtcaggg cgatttcaaa
     7681 agcccggaag ggttttatag cgtccagcgt aatcagttaa aaccagacag ccgttactac
     7741 aaagcgatta atattggttt ccccaatgcc tatgaccgtg cgcatggtta tgaagggaaa
     7801 tacctgatga tccacggcga ttgtgtttcc atcggctgct acgcgatgac caatcagggc
     7861 attgacgaga ttttccagtt cgttactggc gcactggtat ttggtcagcc gagcgtgcaa
     7921 gtgagcattt acccgttccg catgaccgac gccaatatga agcgccataa atattccaac
     7981 tttaaggact tctgggagca actgaagcca ggctacgact actttgagca aacccgtaag
     8041 ccaccgaccg tttctgtggt caatggccgt tacgtggtca gcaagccgtt aagccacgaa
     8101 gttgtgcaac cacaactggc atcaaactac acgctccccg aggcaaaata agcgccattc
     8161 gcctggcata atcttttgcc aggtttcatt gcccgtcagc ggctgtgtcg caatcaccgt
     8221 gaccacatca ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct gatccagcaa
     8281 cgttgccacg ccaaacggcg cgcggcgggt gatccagtgc agattggtcg agcaatacgc
     8341 cattacatag cgcccatccg aaagcagcat gttgaaaacg cccttctgcc gcagttcatc
     8401 cgccagtgag gcgatatatt taaataccgc cgccatattg cccggcgtac gcggataacg
     8461 ctgcgttaat ttatacagca gccagcaaaa ggctttttcg ctgtcggttt tacctaccgg
     8521 gcggaagttg ccggtttcca gtgatttata gcccgtcagt tgtccgttat gggcataagt
     8581 ccagttacgt ccccataact cgcgggtaaa cgggtgagta ttttccaacg ccacctcgcc
     8641 ccgatttgcc tggcgaatat gagccacaac cgaacaggat tttatcgggt agtcctgaac
     8701 aagtttggca atgggggaat taaagctggg ttgtggatct ttaaatgtgc gacagccttt
     8761 accttcgtaa aaggtaatgc cccagccatc tttatgtggc ccggttccac caccacgctg
     8821 caccagcccg gtgaaactaa agcaaatatc ggtagggacg ttggcgctca tcccgagcaa
     8881 ttcgcacatc ggtaaccccc acaacagcgg gagcgcatgg cccccggcga agattgactt
     8941 aagaattttc aggccggatg cggcgtaaac gcattattcg gcctacgcca gacttactta
     9001 accatctctt tttcaattaa ctggatcagg atatggatca ctttaatgtg aatttcctga
     9061 atgcggtcgg cataaccaaa atgtggaacg cgaatttcaa tatccgccgt accagccatt
     9121 ttgccgccat ctttgccggt cagggtaatc actttcattc ccttctcacg cgccgccgcg
     9181 atcgctttga tcacgtttgc agagttacca gacgtggaga tccccagcag tacatcgcct
     9241 tcgcggccta ccgcttcaac atagcgggag aaaatatcat tgaaaccaaa atcgttaccg
     9301 acgcaggaaa tatggctgac gtcagagatg gcaatcgccg ggtagcccgg acggttttca
     9361 cggtagcgac cggtcagctc ttcggcaaag tgcatggcgt cgcaatggga gccgccgttg
     9421 ccgcaggaaa gcaccttgcc accggcttta aagctgtctg ctaacaggac cgccgctcgc
     9481 tgaatggcgt gaatattggc gtcatctttt aaaaagttag ccagcgtttc cgccgcttcg
     9541 ttcagttcgt tacgaataag atcctggtac atgaggatat ccttcagcat aaatgtaata
     9601 gacaaaatgc agtgtaccgg ataccgccaa aagcgagaag tacgggcagg tgctatgacc
     9661 aggacttttt gccctgaagt gtagataaaa acagcaacaa tgtgagcttc gttgtaatta
     9721 tattgtaaac acattgctaa atgtttttac atccactaca accatatcat cacaagtggt
     9781 cagacctcct acaagtaagg ggcttttcgt tatgatgatt ttgagtattc tcgctacagt
     9841 tgtcctgctc ggcgcgttgt tctatcaccg cgtgagctta tttatcagca gtctgatttt
     9901 gctcgcctgg acagctgccc tcggcgtcgc tggtctgtgg tcggcgtggg tactggtgcc
     9961 tctggccatt atcctcgtgc cgtttaactt tgcacctatg cgtaagtcga tgatttccgc
    10021 gccggtattt cgcggtttcc gtaaggtgat gccgccgatg tcgcgcactg agaaagaagc
    10081 gattgatgcg ggtaccacct ggtgggaagg cgacttgttc cagggcaagc cggactggaa
    10141 aaagctgcat aactatccgc agccgcgcct gaccgcagaa gagcaagcgt ttctcgacgg
    10201 cccggtagaa gaagcctgcc ggatggcgaa tgatttccag atcacccatg aactggcgga
    10261 tctgccgccg gagttgtggg cgtatctgaa agagcatcgc ttcttcgcga tgatcatcaa
    10321 aaaagagtac ggcggcctgg agttttcagc ttatgcccag tctcgtgtgc tgcaaaaact
    10381 ctccggcgtc agcgggatcc tggcgattac cgtcggcgtg ccgaactcat taggccccgg
    10441 cgagctgctg caacattacg gcactgacga gcagaaagat cactatctgc cgcgtctggc
    10501 gcgtggtcag gagatccctt gctttgcact gaccagcccg gaagcgggtt ccgatgcggg
    10561 cgcgattccg gacaccggga ttgtctgcat gggcgaatgg cagggccagc aggtgctggg
    10621 gatgcgtctg acctggaaca aacgctacat tacgctggca ccgattgcga ctgtgcttgg
    10681 gctggcgttt aaactctccg acccggagaa attactcggc ggtgcagaag atttaggtat
    10741 tacctgtgcg ctgatcccga ccaccacgcc gggcgtggaa attggtcgtc gccacttccc
    10801 gctgaatgtg ccgttccaga acggaccgac gctcggtaaa gatgtcttcg taccgatcga
    10861 ctacattatc ggcgggccga aaatggccgg gcaaggctgg cggatgctgg tggagtgcct
    10921 ctcggtaggc cgcggcatca ccctgccttc caactcaacc ggcggcgtga aatcggtagc
    10981 gctggcaacg ggcgcgtatg ctcacattcg ccgtcagttc aaaatctcta tcggtaagat
    11041 ggaagggatt gaagagccgc tggcgcgtat tgccgggaac gcttatgtaa tggacgctgc
    11101 ggcatcgctg attacctacg gcattatgct cggcgaaaaa cctgccgtgc tgtcggctat
    11161 cgttaagtat cactgtaccc accgcgggca gcagtcgatt attgatgcga tggatattac
    11221 cggcggtaaa ggcattatgc tcgggcaaag caacttcctg gcgcgtgctt accagggcgc
    11281 accgattgcc atcaccgttg aaggggctaa cattctgacc cgcagcatga tgatcttcgg
    11341 acaaggagcg attcgttgcc atccgtacgt gctggaagag atggaagcgg cgaagaacaa
    11401 tgacgtcaac gtgttcgata aactgttgtt caaacatatc ggtcacgtcg gtagcaacaa
    11461 agttcgcagc ttctggctgg gcctgacgcg cggtttaacc agcagcacgc caaccggcga
    11521 tgccactaaa cgctactatc agcacctgaa ccgcctgagc gccaacctcg ccctgctttc
    11581 tgatgtctcg atggcagtgc tgggcggcag cctgaaacgt cgtgagcgta tctcggcacg
    11641 tctgggtgat attttaagcc agctttacct cgcctctgcc gttctgaagc gttatgacga
    11701 cgaaggccgt aatgaagccg atctgccgct ggtgcactgg ggcgtacagg atgcgctgta
    11761 tcaggcagaa caggcgatgg acgatttact gcaaaacttc ccgaaccgcg tggttgccgg
    11821 gctgctgaat gtggtgatct tcccgaccgg acgtcattat ctggcaccgt ctgacaagct
    11881 ggatcataaa gtggcgaaga ttttacaagt gccgaacgcc actcgctcac gtattggtcg
    11941 cggtcagtac ctgacgccaa gcgagcataa cccggttggt ttactggaag aggcgttggt
    12001 ggatgtgatt gccgccgacc cgattcatca gcggatctgt aaagagctcg gtaaaaatct
    12061 gccatttacc cgtctggatg aactggcgca caacgcgctg gcgaaggggc tgattgataa
    12121 agatgaagcc gctattctgg tgaaagcgga agaaagccgt ctgcgcagta ttaacgttga
    12181 tgactttgat ccggaagagc tggcgacgaa gccggtaaag ttgccggaga aagtgcggaa
    12241 agttgaagcc gcgtaattga ataacggagc cgaaaggctc cgtttcttta tccgctaatt
    12301 atttaaaatt aaagccatcc ggatggtttt ccaggctgcc ggtcaacgcc gcgaacagca
    12361 ccgttttacc atcaatcgaa agcgcatcgt tcacattcaa ccaggtcagt ttctcttgcg
    12421 acgttttctc atcaatagtc gagaacagcc ccgtcatctg attagatttc tcggaccaca
    12481 tcacagcgat acgttgcgag ccacagtcat gcggtttgca cgcgctcatc acctgatacg
    12541 tctcatctcc caacgttacg gtttgtgcag gggtataagt accgcctttc atcacccagg
    12601 caggcagctt atgcccttgc accatctgat taaatgcagc tttggtggtt tcgccctttg
    12661 caaggctgct aatggttaaa tcatcctgcg ccattgcact ggtggcgatg accagtgcgg
    12721 cgactgtcgt tattgcctta aacatcattc ctcccgagct tatcctgccc atgttattaa
    12781 cctcctaaag cctggcgata acacgataat ttgcaaatca gatatttcca atatcagcat
    12841 ttgttatcaa tttattctgc gctaaggcaa ctgcctgata aggtgttaaa gttataaaac
    12901 ccttcgcata aaggagttgt acaccgtgcc tggtttgaag attacgcttt tgcagcaacc
    12961 actggtgtgg atggatggtc ctgccaacct gcgtcatttt gatcgtcaac tggaaggtat
    13021 taccgggcgc gatgtgatcg ttctaccgga gatgtttacc agcggctttg ccatggaagc
    13081 ggctgcttcg tcgctagcac aagatgacgt agtgaactgg atgacagcca aagcgcagca
    13141 gtgcaatgcg ctgattgcag gcagtgttgc attacaaacg gagtctggtt cggttaaccg
    13201 ctttttgctg gttgagccgg gcggcacggt acatttttat gataagcgtc atctgttccg
    13261 catggcagat gagcatctgc attataaagc gggcaatgcg cgagtgattg tggaatggcg
    13321 cggctggcgt attttgccgt tggtgtgcta cgacttacgt tttccggtgt ggtcgcgcaa
    13381 tctcaacgat tatgacctcg ccatctacgt tgccaactgg cctgctccgc gctctctgca
    13441 ctggcaggca ttgctgacgg cccgcgcgat tgagaatcag gcgtatgtgg cgggatgcaa
    13501 tcgcgtcggc agcgatggca acggctgcca ttatcgcggt gacagccggg tgattaatcc
    13561 acaaggcgag attatcgcta ctgccgacgc gcatcaggca acgcgcattg atgcggagct
    13621 gtcgatggtg gcattgcggg aatatagaga aaagtttccg gcatggcggg atgcggatga
    13681 gtttaggttg cggtgagtgg gggctgtatt tagggcgggg aattcagaaa gggatgttta
    13741 gtgtgctgag cgagagttaa ataataaaaa actgggaaga tttaataatc cccccgcagg
    13801 gcaagagcat gaattaaatc tgtgttaaat ttttcatttt gtcagttcga aacccaagct
    13861 ctgagcagga tgcaagcact tcttctatat aacatgtgtt taacgcaacc ttctttcttt
    13921 tcctcttcac cccaccctta atgtctttgc aatctcttaa taaattcagt gccatcttct
    13981 ttattccaga tattatttcg gctgcgtttc ctcttcttat ccggctggca tcttcattca
    14041 tttttacatc taacacccaa tgaagactgt gctcgatcag ccagtgcgct ctgatagcat
    14101 gtgcaaattc tttagcatcc atatcctttg atgaaatata atatcggatg cttacacctt
    14161 ctgctgattt atcttctttc ttctgcctga atgacaatgc tacacaaagc ttttttaatc
    14221 ccttccattc gaattcaaaa tcacaaaatt caggcgttac gttactgaca atatgcaaac
    14281 gtgtttcttt tcttccatga cttatctcct gcgtactaaa cgaatcgcct ttataattag
    14341 aaaacacatt tacaggaaat ttttcctcga atgcatgatg taatttcccc tgattgcctt
    14401 ttactgccag aagataatct gcttttttat ctttgatctt cgaagcgata tctttctgac
    14461 agcccatagc atcaatggtt atcaaatttt tctttaaatc cagtaggtta agcaactctg
    14521 gaatggctgt aatctcatta cttttggctt ccgtttttac ctgccccagt acaacaccat
    14581 tttcgttcga gaatgcactc accatatgga ttgctccttt tctttttccc ttatcaaagg
    14641 agcctcttat ggtctttcca tctattgcta taatttcacc atcagtgatt tcatggcact
    14701 cctgcatcca ttcaataaat atcttttcaa aggccaaact gtcaatgtta ctcacaacgc
    14761 gtgcaatggt gtcatcgacc ggaatgccat tatcaaaatc accatatttc tttagccatt
    14821 caagtctttc atgtccaaaa tcttcaattt cctgccactc atcggcacct gcaattactg
    14881 cacatacggt gagaaacaaa atatcagata atttatgttt aacctttcct tgttgtcgta
    14941 tatcagggat cactgaaata taatcaagca aactttgaat actcacctgg cgtccttttt
    15001 atctttttag ttattccaaa ggttaaattt tgatcacctg taggcgttaa agccaggatg
    15061 aaaatcgtga tttacatcac gattttcatg atcttgccct gccccgtccc ttgctctcat
    15121 gtctttctgt atgtatttta tatttttctc tttctggtgt tgttcattat gaccatttac
    15181 caatccagtc aaaaacttca tttttcaaat tgttatcata ataatcaaca tcataatagt
    15241 ctccataatt aattatttta taactagcct tcttgttttt atcactaaaa tacacccgtt
    15301 gttttgtagc gcagaactcc atattctcca gtttactaat gttttttaat cgcacgctat
    15361 aaagaatatc attgaaaaa
//
LOCUS       DABSYT020000104        15128 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.104, whole genome shotgun sequence.
ACCESSION   DABSYT020000104 DABSYT020000000
VERSION     DABSYT020000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.104"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            86..2269
                     /gene="espX6"
                     /locus_tag="ID725_RS19380"
                     /old_locus_tag="pgaptmp_003876"
     CDS             86..2269
                     /gene="espX6"
                     /locus_tag="ID725_RS19380"
                     /old_locus_tag="pgaptmp_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HGV7852371.1"
                     /translation="MGSLFNIYKDIFPTLGMYSGLKACHEKNNLPFDINTEIETIQKQ
                     INYDINHLNDGLIKRVLNLFIHLISNPDNLELTLNRYSSTTEQIIGRTKRNGLHEFDD
                     GDLKIIFNRQDDNESVLTVKDKDKDKDISHHCNVKTEQLQQFIKIMEQKAQLPIYIDK
                     NNLKESIFSVLHNDPQQVDKDQHLPCEKFLKHACKSSNSFEVKLDATHQYQHLNNFMI
                     SLDPVENQLTIRDNNNKTETFSFTNLQWENLLQYYKENHQQPNIAGSRNLTDNIDKIK
                     NTISTSEIIECASPEIRSSVLNDLYSIANFLPDNNLTPNESWKRFCETCERFYVAQKS
                     ITGDKSERLTRKLSISDAGITMTFKIGDVVINTISTAIPEDATGQRCIEGLNLAEMDL
                     TDIDLSKMALRNVNFNGSILRNAKFSGTICEGVDFTDCDLRNAEFENASLENNDFRKV
                     RHLTYVNFKNANLRNSNFNGKVLTGVTFTGSDLSNAYLEHIDFTTVILYETSKIPGIP
                     GTPQIPGTPKVILTGAILNYSDLSGKDLSEYNLTGILCMYTNFSNANLTNCKISNANF
                     SNAKFYNTNCTGANCSNILFDYAWFDNTIFIKTLFKNTCFYNVRAKNVYLEGAYLNND
                     NIVNQANNSTEKQSIDSTDKQANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDST
                     DKQAKNSTEQQDSNSFNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
     gene            2535..3377
                     /locus_tag="ID725_RS19385"
                     /old_locus_tag="pgaptmp_003877"
     CDS             2535..3377
                     /locus_tag="ID725_RS19385"
                     /old_locus_tag="pgaptmp_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGV7852372.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            3445..4623
                     /gene="yjiJ"
                     /locus_tag="ID725_RS19390"
                     /old_locus_tag="pgaptmp_003878"
     CDS             3445..4623
                     /gene="yjiJ"
                     /locus_tag="ID725_RS19390"
                     /old_locus_tag="pgaptmp_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7852373.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            4834..10075
                     /gene="iatD"
                     /locus_tag="ID725_RS19395"
                     /old_locus_tag="pgaptmp_003879"
                     /pseudo
     CDS             4834..10075
                     /gene="iatD"
                     /locus_tag="ID725_RS19395"
                     /old_locus_tag="pgaptmp_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(10232..10827)
                     /gene="kptA"
                     /locus_tag="ID725_RS19400"
                     /old_locus_tag="pgaptmp_003880"
                     /pseudo
     CDS             complement(10232..10827)
                     /gene="kptA"
                     /locus_tag="ID725_RS19400"
                     /old_locus_tag="pgaptmp_003880"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            11032..11715
                     /gene="yjiH"
                     /locus_tag="ID725_RS19405"
                     /old_locus_tag="pgaptmp_003881"
     CDS             11032..11715
                     /gene="yjiH"
                     /locus_tag="ID725_RS19405"
                     /old_locus_tag="pgaptmp_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGV7852374.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            11712..12173
                     /gene="yjiG"
                     /locus_tag="ID725_RS19410"
                     /old_locus_tag="pgaptmp_003882"
     CDS             11712..12173
                     /gene="yjiG"
                     /locus_tag="ID725_RS19410"
                     /old_locus_tag="pgaptmp_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGV7852375.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            12186..13358
                     /gene="iadA"
                     /locus_tag="ID725_RS19415"
                     /old_locus_tag="pgaptmp_003883"
     CDS             12186..13358
                     /gene="iadA"
                     /locus_tag="ID725_RS19415"
                     /old_locus_tag="pgaptmp_003883"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGV7852376.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            13480..14334
                     /gene="hypT"
                     /locus_tag="ID725_RS19420"
                     /old_locus_tag="pgaptmp_003884"
                     /gene_synonym="qseD"
     CDS             13480..14334
                     /gene="hypT"
                     /locus_tag="ID725_RS19420"
                     /old_locus_tag="pgaptmp_003884"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGV7852377.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(14327..14719)
                     /gene="iraD"
                     /locus_tag="ID725_RS19425"
                     /old_locus_tag="pgaptmp_003885"
     CDS             complement(14327..14719)
                     /gene="iraD"
                     /locus_tag="ID725_RS19425"
                     /old_locus_tag="pgaptmp_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGV7852378.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(14716..14799)
                     /gene="idlP"
                     /locus_tag="ID725_RS19430"
                     /old_locus_tag="pgaptmp_003886"
     CDS             complement(14716..14799)
                     /gene="idlP"
                     /locus_tag="ID725_RS19430"
                     /old_locus_tag="pgaptmp_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGV7852379.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(14915..15058)
                     /gene="ytiD"
                     /locus_tag="ID725_RS19435"
                     /old_locus_tag="pgaptmp_003887"
     CDS             complement(14915..15058)
                     /gene="ytiD"
                     /locus_tag="ID725_RS19435"
                     /old_locus_tag="pgaptmp_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HGV7852380.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         4170 a         3651 c         3498 g         3809 t
ORIGIN      
        1 ataagtttct gtgcaaagct tatctttccc caccccctga ttattttaga tttagaagaa
       61 tatttcacag ttcaaggata aaaatatggg ctccctattt aacatataca aagatatttt
      121 cccaacactc ggcatgtatt cgggactaaa agcttgccat gaaaaaaaca acctaccatt
      181 tgatattaac acggaaattg aaaccataca aaaacaaatt aattatgata taaatcattt
      241 gaatgatggt ttgattaagc gtgtactgaa tctttttatt caccttatct ctaatcccga
      301 caatcttgaa ttaaccttaa atagatattc atcaacaaca gaacaaatca tcggcagaac
      361 caaaagaaat ggtttacatg agtttgacga tggcgatcta aaaataatat ttaatcgaca
      421 agatgataat gaaagcgtat taactgttaa agataaagat aaagataaag atataagtca
      481 tcactgcaat gttaaaaccg agcaactgca gcagtttatt aaaataatgg aacaaaaagc
      541 gcaactacca atctatattg acaagaacaa tttgaaagag agtattttct ctgttttgca
      601 caatgaccca caacaagtag ataaagatca acaccttccc tgtgaaaagt ttttaaaaca
      661 tgcctgcaaa agttcaaatt catttgaagt gaaattagat gccactcatc aatatcaaca
      721 cctgaataac ttcatgattt ctttggaccc agtagaaaat caattaacca tacgggataa
      781 caataacaag actgaaactt tctcgtttac aaacttacaa tgggaaaatt tgctgcaata
      841 ctacaaagaa aaccaccagc agccaaatat agcaggatca cgaaatctca cggataatat
      901 agataaaatt aaaaatacaa tatccacctc tgaaattatt gaatgcgcct ctcctgaaat
      961 aagaagtagc gtcctgaacg atctttatag cattgctaat ttcctcccgg acaataatct
     1021 gaccccaaat gagagctgga aaagattttg tgagacatgc gagcgctttt acgttgctca
     1081 gaagagtatc actggagata agagtgaacg tcttacgcga aaactctcta tctctgatgc
     1141 aggaattaca atgaccttca agataggtga tgttgtcatc aatactatta gcactgctat
     1201 tcctgaagat gcaacgggtc aacggtgtat cgaagggttg aatttagcag agatggattt
     1261 aaccgacata gacttgtcga aaatggcgct aaggaatgtc aattttaatg gcagcattct
     1321 tagaaatgcc aagttctccg gtacgatctg tgaaggcgtg gattttaccg attgtgatct
     1381 gcgtaatgca gaattcgaaa atgcctcatt agaaaataat gattttcgta aagttcgcca
     1441 cttgacttat gtaaatttca aaaacgcaaa tctacgaaac agtaacttca acggaaaagt
     1501 tctcactggc gtaaccttta ctggaagtga ccttagtaac gcgtatcttg aacacataga
     1561 tttcacaacc gtgattctat atgaaacatc taaaatacct ggaatacctg gaacacctca
     1621 aataccggga acacctaaag taattcttac tggcgcaata ctaaattatt ccgatctatc
     1681 gggaaaagat ctttcagaat ataatcttac tggtattctc tgcatgtata ccaacttttc
     1741 aaacgctaat ttaacaaatt gtaaaatctc taatgcaaac ttttcgaatg caaaattcta
     1801 caatactaat tgtactggtg caaattgttc gaatatccta tttgactacg catggtttga
     1861 caatacaata tttataaaaa cgctttttaa aaatacctgt ttttacaatg tcagagcgaa
     1921 aaatgtctat cttgagggag catatctgaa caatgataat atcgtgaatc aagccaataa
     1981 cagtaccgag aaacaatcca ttgacagtac cgataaacag gccaatgaca gtacggtgca
     2041 acaatccatt gacagtacgg tgcaacaagc caatgacagt accgataaac aagccaatga
     2101 caatatcgat aaacaggtca atgacagtac cgataaacaa gccaagaaca gtaccgagca
     2161 acaggacagt aacagtttta atcaagcccg tttaaagaaa gaagtgaata ggagattttc
     2221 cattccgggt ttaacgtctt atcagccaac atatatagtt gaagaatagt tcattaatca
     2281 cccaaacaga gtgggtatgg cagacgatat agattaacat ttaaacgtta accgcctcat
     2341 ctatcattca tagatgaggc gcatttatca gtcgatgtaa gctgaaaatg ttctgccatc
     2401 cagagacaac cgccgccccc tgcggcgttt actatttcat cgcccgcgtt aagaattcat
     2461 taagcttcat ccgtatactc cttatctgtc gcactgaatt tgacgagaaa aaagcaataa
     2521 taaggagcaa taagttgaaa aagcgcatca cctttatcgt ttttctttgc gccatcgtgg
     2581 cggcaagcct gttttttgtg caatcctgcg tccgtaaaag ccagcatgtc gcagggtttc
     2641 agaattatca ggccactatc gatggtaaag agattacagg agtaactaaa aatatctcct
     2701 cactgacctg gtcagcgcag agcaatacct tatttagcac tatcaacaaa cctgcaacca
     2761 tcgttgaaat gacgaccgag ggcgacttga tccgcaccat tccgctcgat tttgttaagg
     2821 atctcgagac gattgaatac attggtgata ataaatttgt catcagtgat gaacgtgact
     2881 acgccattta tgtgatttca cttaacgctg actcggaagt gagcatcctc aaaaaaatta
     2941 aaatcccatt gcaggaaaca ccaactaact gtggctttga agggctggct tactcctctc
     3001 aggaccatac cttctggttt tttaaagaga aaaatccgat agaggtctac aaagtaaccg
     3061 ggctattgcg tagcgatgag ttgcatatca gcaaagataa aacgttgcag cgtcagttta
     3121 ctttagacga tgtttctggc gcggaattta acccacagaa aaatacgttg ctggtgttgt
     3181 cacatgagtc acgcgccctg caggaagtga ccgtgagagg tgacgtgatc ggcgaaatgt
     3241 cgttaaccaa aggtaagtat ggcctttcac acaacatcaa gcaggcggaa gggatagcga
     3301 tggatgatag cggcaacatc tatatcgttg gcgaaccgaa tcttttttat cgctttacgt
     3361 cgacaaaatc acgataacag tctggcaggt tctccttttt tgttattaac ttgttaactc
     3421 aaaataatac aaaccaggca gaacatgaat tcgacatcgc atcccgtaga acgtttttct
     3481 ttcagcaccg cgttattcgg gatgctggtt ctgaccttag gtatgggttt aggccgcttt
     3541 ctctatacgc cgatgctgcc agtcatgctg gcggaaggcg agttttcatt tagcgaactc
     3601 tcatggatcg ccagtggtaa ctatgccggg tatctggcgg ggagcctgct gttttcattc
     3661 ggcgcgtttc atttaccctc acgcctgcgc ccgttcctgt tagcttccgc cctcgcaacc
     3721 ggattattaa tcctcgcgat ggcgtggctg ccgccgtttc ttctggtttt catcattcgc
     3781 tttctggcgg gagtcgccag cgccgggatg ttgattttcg gctcaacact catcatgcaa
     3841 catactcgcc atccctttgt ccttgcggcg ctattttctg gtgttggcgt cggcatcgct
     3901 ctgggtaatg aatatgtgct ggcaggcctg cattttgccc tctcttcaca aacgttgtgg
     3961 caaggtgccg gagcactttc tgccattata ttgcttgctc tggcgctgct catcccgtcg
     4021 aataaacacg ttatcccgcc agcgccattg gcaaaaatcg cgcaacaacc catgagctgg
     4081 tggttactgg cgattctgta tggtctggcg ggttttggtt atatcatcgt cgccacctac
     4141 ctgccgctca tggcgaaaga cgcgggccag cctgtgttga cggctcacct ctggacactg
     4201 gttggcttgt cgattgtccc aggttgcttt ggctggctgt gggcagccaa acggtgggga
     4261 gcattacctt gcctgaccgc gaatttgctg gtgcaggcga tctgcgtgct gttaaccctc
     4321 gccagcagct ctcctttatt actcatcatc agcagtattg gttttggcgg cacctttatg
     4381 ggaacgacct cgctggtgat gaccatcgcc cgccagctta gcgtgccggg aaatcttaac
     4441 cttttgggct ttgtgacact catttatggt atcgggcaaa ttcttggccc ggcgctgacc
     4501 agtatgctcg gcaacggaac gtcggcgctc gccagcgcca cactctgcgg cgcagcggcg
     4561 ctatttatcg cagcattaat ctgcgggatg caaatattca aattgcatac gaattattct
     4621 taagtaattt aactaattta acttttcagc acatcacgta aaataattaa aatatccttt
     4681 ttctttggag gtactcagcc accgccggta ttattcctgg tttagcaatt ccacggctaa
     4741 ttcattattt ctggtcatat tatagtcagg atcgcattat atctccgtgc tagaaaaaac
     4801 aagtttaatt ctgttttttc acggaaggtc actatggctt cgacaaatgc gcatcaaata
     4861 aaaaataatg atcaaaactc tttatgtggt ctgggtgaca aaatacgccg cctgaccgcc
     4921 ggagtttgtt tatttacaca attttttttc cccgtcatgg cgacggcaca aaatgtggta
     4981 catgctaaac ctcagacgac agtttcatct gcccccccct ctactagaaa ataatactgt
     5041 gccttacacg cttggtgcgc tggaatcagc gcaaagcgtt gctaatcgct tcggtatttc
     5101 actggaggag cttcgtcgtc ttaatcagtt ccgtactttt gctcgcggct ttgataacgt
     5161 gcgccagggt gaagaactgg atgttccggc aacaacctcg cagaaaagcc atgagcaaca
     5221 aaatgccgta ccgcctgcga atggcgaaaa cacgctggag aatcaaatag ccagcacctc
     5281 gcagcgagtt ggcactctgc tttcacaaga tatgaatagt gagcaggcca gcggcatggc
     5341 gcgtggttgg gcgtcttcag aagcctcagg cgcgatgact gattggttaa ataactttgg
     5401 tactgcgaga atctctctgg gtgtggatga agattttagc ctgaaaaatt cgcaattcga
     5461 cttcctgcat ccgtggtatg acacacctga ttatctgctc ttcagccagc atacccttca
     5521 ccgaacagac gatcgtaccc agatcaacac cggtttgggc tggcgtcatt tcacctccag
     5581 ctggatgtca ggcatcaacc ttttttttga ccacgacctg agccgctatc actcccgcgc
     5641 agggcttggc gcagaatact ggcgtgatta tctgaagttg agcagcaacg cttatatcgg
     5701 cctgaccggc tggcgtagcg caccagaatt ggataacgac ttcgaagccc gcccggccaa
     5761 cggctgggat ttacgcgcgg aaggctggtt acctgcctgg ccacaactgg ggggaaaact
     5821 ggtctatgaa caatactatg gcgatgaagt ggcgctgttt gacaagaatg atcgtcaaag
     5881 taacccccat gctattaccg caggcctcaa ctataccccc ttcccgcttc tgactctcag
     5941 tgcggaacag cgtcagggga agcaaggtga aaatgacaca cgttttgccg ttgatctgac
     6001 ctggcaaccc agcagttcaa tgcagaaaca gcttaatccg gacgaagtgg ccggacggcg
     6061 cagtctggcc ggtagtcgtt atgacctgat tgatcgcaac aacaacatcg ttctggaata
     6121 ccgcaagaaa gagctgattc gcctgagtct gctggatccg gtgaaaggga agtctggaga
     6181 aataaaaccg ctggtttcct cgctacagac caaatatgcc cttaaaggct ataacatcga
     6241 agccgctgcg ctggaagctg ccggaggtaa agtcagcacg tctggaaaag atatcacggt
     6301 cacgctgcca ggttaccgct tcactaacac cccagaaacc gataatacat ggtcgataga
     6361 cgttaccgcc gaggatgtaa aaggtaacct gtcacggcat gaacaaagca tggtagttat
     6421 tcaggctccg acattaagcc agaaagattc tctgttatcc gtcaatccgc taaccgtggc
     6481 tgcagataaa aaatcgacga ccacattgac cgttactgcg cacgattccg acggaactcc
     6541 ggtgccgggg ctggcgctgc aaacccgcag tgaaggcgtt caggatatca ccctgtctga
     6601 ttggacagat aacggtgatg gtagttacac acagatactg accgccggaa cgacatcagg
     6661 ttcagtaaca ctgacgccgc aaattaacgg tgagagtgcg gtaaaagaat ccatcgtcgt
     6721 taatatcgtc cctgttgtct catcccgcga ccattcatca ataacaattg ataacgtatc
     6781 gtattatgcc ggagacgaca tcaaggttag ggtggaactg aaagacgata gcaatcaacc
     6841 ggttgcatat caaaaagagg aattggtaaa agccgttact gtcgaaaaca gcaaacctgg
     6901 cgccacgatt gtctggcacg aagagcagcc gggggtttat gccgcgaatt atccggccta
     6961 taagcaaggg actgcactaa gggcacaact tagccttcac aactggaatg ctccactgca
     7021 atcgcatatt tataacattg aggcaaacca gaataaggct cgcgttgcca cattatcagc
     7081 gacaaataat gacgtttacg ccgataaaaa gacatttaat accctcacga tcaacgtcac
     7141 tgatgagagt gataatcccc tgacaaatca tcaggtcacc tttaagaatg aaaaaggaag
     7201 cgcggagttt gtcgaaccgc cgcagcaaaa tacggatgca tatggtgttg ccacaataaa
     7261 catggtaagt caggttgcgg aagaaaatac gattagcgcc acgctgccaa atggtttttc
     7321 acaacggata attgcgaaat tcgttagcga ttcgagtacg ccaaaattca aacaactggt
     7381 tgccgatcca gataccatta ttgctggcaa cagccagggc agtactctga ccgccatcat
     7441 cacagacttt cataacaacc cgttaaaaga tatgaaagtg aattttgtgg cacctggtgg
     7501 ctcgcaactg gacaacacga ccgccacaac agaccagtcc ggtattgtgc gggtgcacct
     7561 gaccagttca aaagctggta gctattccgt cgatgcctcg cttgaggtgg ataaaaatat
     7621 tcaccagtcg gtcacgatca ccgtggtccc aaacagggaa caatcggtaa tgaccttgaa
     7681 tgccgggtcg ggcagtgcga tcgctaacaa tacaaatatc gttaccctga ctgccagtgt
     7741 gaaagatgtt tatggacacc cgttgccgga tgaggatgtg aaatttacct tgccagcctc
     7801 catgaccggg aacttcacgc taagtagtga aaccgcccgc accgatgcaa acggtgatgc
     7861 cgtggtcaca ttgcgaggca caaaagcggg tgagtttaca gttacggcga cgctgaccag
     7921 aaataatacc gttgcttatc agcaagtcac ttttattggg gatacaaaca gtgcgcagct
     7981 ccagccgctg actgcctcat taaattccat tgttgcgggt aacagtacgg ggagtaccct
     8041 gacggcaacg atcctggacg cttaccaaaa tccgcttaaa gaccagttgg tcactttcca
     8101 gagtaacgat gtcactctaa gcgaaacaga agtcaccacc aatacgctgg gtcaggcgac
     8161 ggtaacaatg accagcaata ttgccggaca acataacgtc gtggtgagcc ggaaagcgca
     8221 agcttccgat aataaaacgt ttagtttatc agtgctaccg gatgaaagtt cggcgaaggt
     8281 aataagtata accggagccg aaaaaacgat aacggtgggc gaaaacatca cgctacggat
     8341 actcgtccag gacgcgttta acaatgtaat cgcgggtcaa cgcgtcagat taagtgcgca
     8401 gccaacaact aacattacga taggcgatac ggcttacacc gataataacg gttatgcgta
     8461 cgttaacctt ctcagcaccc aacctggggt ttatcaggtg acggcaacgc tggacaataa
     8521 cagtagtagt aaggttgacg tgaatgtggc aaatggcaaa ctcgagttaa catcatcgaa
     8581 accagaaact acggtccata atagtgaggg tattacgctg accgcaacgg cgagaaatgc
     8641 gcggggtgaa ttgatgccag ggcaaattat cacctttagc gtaacgcctg aaggtgcaac
     8701 gctaagcaat acaggggaag tccttactga ccagtcaggt caggccaaag tgacgctgac
     8761 cagtgacaaa gtgaatgtct ataccgttac ggccataatg ggcaaagatg ttcccgttca
     8821 gagccaggta acggttgcgg ttaaggcaga tgctaaaacg gcacatgttg tgagcgtcgt
     8881 ggcttctcct gacaccatca ccgccgacgg catcgatagc agcaccatca cttcacgagt
     8941 agaagatgat tacggattcc cggttgaagg tgtcgatatt agtcatggct tagacaccaa
     9001 aggcagcccg gtagttaata ttccaactac gcgtaccgat cagtccgggc aagtcacggc
     9061 gacaataacc agtacattgg cagaaacctt aacagtcaat gtgcaagttc ctggcacagc
     9121 caaccaatcc gcaaccatta cattggttgc cggcacggcc gatgaaagta agtcaatttt
     9181 gaaatccgat gttgacactc tgaaggctga ctaccagcag agcgcaaaac ttacgctaac
     9241 attgcaagac aagtacggta acccgatagt gacgtctgat catctggaat ttgtccagtc
     9301 aggccccttc gtgaactttc tcaagttgag cgatattgat tacagccaaa gaaattatgg
     9361 cgagtacacc gtgactgtca ctggcggaaa agagggaaca gcgacactca ttcccatgct
     9421 gaacggggtt catcaggcaa acttaagcat atcgctgaat ctcatccaat cgataaaaga
     9481 aatgtccggt catgtcactg caaacaacca taccttctcc acggctaaat tcccgagcga
     9541 aggctttgca ggagcgtatt acacactcaa caatgataac tttgaagcgg gtaaaaccgt
     9601 tgatgattat atgttttcaa gttcacaggg ttgggtgtct gtcgatgctt cgggtaaagt
     9661 ttctttcgca aatatcggcg atcaaacgtc agtcacaata agcgctgttc cccgacaagg
     9721 aggtacaacc taccagacct taattaagct gaaaggctgg tgggtgaata atggaaatca
     9781 taccaatatc tggctagctg ccaatgcgct ctgtcatgct aaaaatgatg gatataatct
     9841 tcctggcatc acacatttga cgtctggcga aaacaaacgc acgcagggat cactgtatgg
     9901 tgaatggggg aacgttggag cgttttccag taattcgcaa tttacaccgg gagcttactg
     9961 gacaagtgaa tctgatgatt acagtcggca ctactatgtg cagatgctaa ccggtatgac
    10021 cggaagcgac gctgattcca gcccccaact gaccgcctgc cgtaaatcac tttaatttca
    10081 gcagttgtta ttaaaggata cccttttacg agggtatcct ttcagactga taacaaacgc
    10141 tacattgccg gatgtgctgc gcttatcagg cctatataac ccctgcaata tattgaattt
    10201 attgtttttg taccccagcc ggcataaacg actactcaag aaactcaaca gcaaccgttg
    10261 atgtcagcca gaccccgttt tccgcttgcc agaaagggat gcctcgtttc gccatttcct
    10321 gtgctttgac ggttaagaca accggcgatc cgtgtcttgc tcccactttg cgtgccgttg
    10381 cttcatcagc agagagatgc acgtaatgac gatcccctgc aatcagcccc tgttttttta
    10441 tctcgtcaag gaaacggctt gccgtaccgt ggtagagaaa ctgtggcggt gttttttcgg
    10501 caaaggaaat agcgacctgc gaagtcgaat gcccctgaac cgcgcggatg cacctaccat
    10561 cactggaata actaaaacgc ttttatcgct ggttgcgacc acggtatcca gcaatgcacg
    10621 ggttaatcgc ttgcccgctt tttgggcaca aagtatgagc ttatcgatat tcgcccatcc
    10681 ttcacggtcc agtacgatac caatcgcctc cggtttgtgt cggaggacaa aacttaaaaa
    10741 cttgctcgta tccgccagct ctttttcgtt gtactttgcc atcgcgtttt cgtccctgga
    10801 ttttctgttt ttatttacca atgtcattgt cactgggttt atgaattttc tgcaactcaa
    10861 tcctgcgcag aaccttttcc acacagcgta atttttcccc tatgcaaaaa acgcatagcc
    10921 agaaacatac ttgcattttc cccataccca catggctgat aaggttttta agttccagga
    10981 attttcctgg ctcaagcaac acacaaacac aaaaaaacaa tggggattgt tatgacacaa
    11041 caaggggatg ctgtagccgg ggagctggcg acagaaaaag tgggcattaa agggtatctg
    11101 gctttttttc tcaccataat ctttttttcc ggtgtttttt ccggaacaga cagctggtgg
    11161 cgcgtttttg atttttccgt tctgaacgga tcgtttgggc aactgcctgg tgcgaatgga
    11221 gccaccacct catttcgcgg cgcgggtggc gcgggggcta aagatggctt tctctttgcg
    11281 ctggagctgg caccgtcagt cattctctct ctggggatta tttcaattac cgatggtctt
    11341 ggtggattac gcgccgcgca gcagttaatg acacctgtgc taaaaccgtt gttgggcatt
    11401 ccagggatct gctcgctggc gctgatcgct aacttgcaaa acactgatgc cgccgccggt
    11461 atgaccaaag agctggcgca ggaaggtgaa attaccgaac gcgataaagt gattttcgcc
    11521 gcatatcaaa ccagcggtag cgccatcatc accaactact tctcttccgg cgtcgcggtg
    11581 ttcgcctttc tgggcacgtc cgtaatcgta cctttagccg tcatcctggt gtttaaattt
    11641 gtcggagcca acattttgcg cgtctggctt aacttcgaag aacgtcgcaa cccaacgcaa
    11701 ggagcgcaag catgacaact caggtccgca aaaacgtcat ggatatgttt atcgatggcg
    11761 cgcgccgtgg ctttactatc gccacgacca acctgctgcc aaacgtggtg atggcgtttg
    11821 tcatcattca ggcgctgaaa attaccggcc tgctcgactg ggtggggcat atctgtgaac
    11881 ccgtaatggc gctgtgggga ttacccggtg aagcggcaac cgtcctttta gccgcattaa
    11941 tgagtatggg cggcgcagtc ggtgttgccg ccagtctggc aaccgctggc gcattaaccg
    12001 gtcacgatgt caccgtttta ctgcccgcga tgtatttaat gggcaacccg gtgcaaaatg
    12061 tcggtcgctg cctggggaca gccgaagtga atgccaaata ttacccgcat atcattacgg
    12121 tctgcgtgat taacgcattg ctgtcgatct gggttatgca gcttattgtt taacaaggag
    12181 ttaccatgat tgattatacc gcagccggtt ttaccctgct gcagggagcg catttgtatg
    12241 cgccggaaga tcggggaatt tgcgatgtcc tcgtcgctaa cggcaaaatt atcgccgttg
    12301 ccagtaatat cccttctgac attgtaccgg actgcacggt tgtcgatctc agcgggcaga
    12361 tcctctgccc aggttttatt gatcaacacg tccatttgat tggcggtggc ggcgaagcag
    12421 gtcccacgac gcgcacgccg gaagtggcgc taagtcgcct gacggaagcg ggcgtcacgt
    12481 cagtggttgg tctgctgggc accgactcta tctctcgcca cccggaatcc ctgctcgcca
    12541 agacccgtgc gctcaatgaa gaaggcatca gcgcctggat gctgaccggc gcttatcatg
    12601 tcccttcccg caccattacg ggttccgtgg aaaaagacgt ggcgattatc gatcgtgtga
    12661 ttggcgtgaa atgtgccatc tctgatcacc gttctgccgc accggacgtt tatcacctgg
    12721 ctaatatggc ggcagaatcc cgcgttggcg gtttgctcgg cggtaaacct ggcgtcaccg
    12781 tgttccacat gggcgacagt aaaaaggcat tacagcctat ttatgacctg ctggaaaact
    12841 gcgatgtgcc gatcagcaag ctgctgccga cccacgttaa ccgcaacgta ccgttgtttg
    12901 agcaggcgct ggagttcgcg cgcaaaggcg gcaccatcga tatcaccagc agcattgacg
    12961 aaccggtcgc ccctgccgaa ggtattgtcc gcgccgttca ggcgggtatt ccgctggcac
    13021 gcgtcaccct cagctccgac ggcaacggta gccagccgtt cttcgatgat gaagggaatt
    13081 taacccatat cggcgttgca ggttttgaaa cgttgctgga aaccgtgcag gtgctggtta
    13141 aagactatga tttcagtatc agcgatgccc tgcgcccgct caccagtagc gtagccggtt
    13201 tccttaacct gaccgggaaa ggcgaaattc tgccaggcaa tgacgcagac ttactggtca
    13261 tgacgccaga attgcgcatt gagcaggtat acgctcgcgg caaactgatg gtcaaagacg
    13321 gcaaagcctg cgtgaaagga acgtttgaaa cggcttaaac ggttgtcagc cgccctaatg
    13381 gacgtagaat gcccccaagg gcggctgaca gagtaaaacg taatggatga ctgtggtgcg
    13441 gttttgcata atattgaaac caaatggctt taagattttc tgaccctgga aaaatgccgc
    13501 aatttttccc aggcggcagt cagtcgtaac gtctcgcaac cggcattcag ccgccgcatc
    13561 cgcgcgctgg aacaggcgat tggcgttgaa ttgtttaacc gccaggtgac gccgctgcaa
    13621 ctctcagaac aagggaaaat cttccattcg cagatccgcc atctgttgca acagttagaa
    13681 agcaaccttg cagagctgcg tggcggcagc gattacgcgc aacgtaaaat caagatagcc
    13741 gctgcacact ctctttccct cggtctgtta ccgtccatta tcagccagat gccgccgctc
    13801 tttacctggg cgattgaagc cattgatgtc gatgaagcgg tcgataaact gcgtgaagga
    13861 caaagtgact gtattttttc ctttcacgac gaagatttgc tggaagcgcc gtttgaccac
    13921 attcgcttat ttgaatctca attgttccct gtctgcgcca gtgacgaaca tggagaagca
    13981 ctttttgacc tcgtgcagcc acactttccg ttactgaatt acagccgcaa ctcctacatg
    14041 gggcgattga ttaatcgcac cctgacgcgc cacagtgagt taagtttcag cacctttttt
    14101 gtctcttcga tgagcgagct tttaaagcag gttgccctcg acggctgtgg gattgcctgg
    14161 ctgccggagt atgccatcca acaagaaatt cgcagcgggc agctcgttgt gctgaatcgg
    14221 gacgaactgg tgatcccgat tcaggcctac gcataccgga tgaatacccg catgaatcca
    14281 gttgccgaac gcttctggcg tgaactgcgc gagctggaga ttgtgcttag ctgacattct
    14341 caagcgtcgc actgcgccaa ccgccattat cccagaccag atcaaacgtt agcggttcat
    14401 cgtcgtggta cagcattatc tcaaggctga tcgccagagt gtggcagtac gcttctttca
    14461 aattaacctg caacgccgca atccgtggct catgaaataa cagtgcagaa cgcagctgac
    14521 ggcaatacca gctctggaga tgtttgtcgc tcgggttatg cacttgccac tcgggcatgc
    14581 ctgaaacaag taatgacggt aatgtcgaat gaggagaatt aaaaagagtg aggagatcgc
    14641 tgcataccga tttacgcagc aatgacgttt tattgcctga aatttcaggt aaaacagcct
    14701 gaagtgattg gcgcatcatt ttgcgcactc ctgacgttta gcaacatatg cggcttgccc
    14761 tgaacaccgg gcaccactgt attgatgctc attttccata gccattccct tttgttcatt
    14821 acatccatgt cagtctgaga caggcctgaa tcctgtcagc aaagtataaa accttacttg
    14881 cctatgaata tctactgact tttcaggaaa aaagtcatga tttgataaaa atcatcaaac
    14941 tcaaaaaaaa accgctagta tattctgaat attccgaata gttacgtaga tgaaaatact
    15001 tgtcaccgct gctacttaat tccggtggga aattatttat ttccgcataa tcagccatgt
    15061 attatttgaa acaattagtt tgtaaataat ataaattgat aacatatcaa atacatcaaa
    15121 aacaccat
//
LOCUS       DABSYT020000105        15073 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.105, whole genome shotgun sequence.
ACCESSION   DABSYT020000105 DABSYT020000000
VERSION     DABSYT020000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.105"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            137..1390
                     /gene="amiC"
                     /locus_tag="ID725_RS19440"
                     /old_locus_tag="pgaptmp_003888"
     CDS             137..1390
                     /gene="amiC"
                     /locus_tag="ID725_RS19440"
                     /old_locus_tag="pgaptmp_003888"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGV7852381.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(1464..1540)
                     /locus_tag="ID725_RS19445"
                     /old_locus_tag="pgaptmp_003889"
     tRNA            complement(1464..1540)
                     /locus_tag="ID725_RS19445"
                     /old_locus_tag="pgaptmp_003889"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1504..1506),aa:Met,seq:cat)
     gene            complement(1574..1650)
                     /locus_tag="ID725_RS19450"
                     /old_locus_tag="pgaptmp_003890"
     tRNA            complement(1574..1650)
                     /locus_tag="ID725_RS19450"
                     /old_locus_tag="pgaptmp_003890"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1614..1616),aa:Met,seq:cat)
     gene            1859..2956
                     /gene="mltA"
                     /locus_tag="ID725_RS19455"
                     /old_locus_tag="pgaptmp_003891"
     CDS             1859..2956
                     /gene="mltA"
                     /locus_tag="ID725_RS19455"
                     /old_locus_tag="pgaptmp_003891"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGV7852382.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            3195..4001
                     /gene="tcdA"
                     /locus_tag="ID725_RS19460"
                     /old_locus_tag="pgaptmp_003892"
     CDS             3195..4001
                     /gene="tcdA"
                     /locus_tag="ID725_RS19460"
                     /old_locus_tag="pgaptmp_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGV7852383.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(4052..4495)
                     /gene="csdE"
                     /locus_tag="ID725_RS19465"
                     /old_locus_tag="pgaptmp_003893"
     CDS             complement(4052..4495)
                     /gene="csdE"
                     /locus_tag="ID725_RS19465"
                     /old_locus_tag="pgaptmp_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGV7852384.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(4495..5700)
                     /gene="csdA"
                     /locus_tag="ID725_RS19470"
                     /old_locus_tag="pgaptmp_003894"
     CDS             complement(4495..5700)
                     /gene="csdA"
                     /locus_tag="ID725_RS19470"
                     /old_locus_tag="pgaptmp_003894"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGV7852385.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5892..6119
                     /gene="ygdI"
                     /locus_tag="ID725_RS19475"
                     /old_locus_tag="pgaptmp_003895"
     CDS             5892..6119
                     /gene="ygdI"
                     /locus_tag="ID725_RS19475"
                     /old_locus_tag="pgaptmp_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGV7852386.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            6470..7387
                     /gene="gcvA"
                     /locus_tag="ID725_RS19480"
                     /old_locus_tag="pgaptmp_003896"
     CDS             6470..7387
                     /gene="gcvA"
                     /locus_tag="ID725_RS19480"
                     /old_locus_tag="pgaptmp_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGV7852387.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            7406..7801
                     /gene="ygdD"
                     /locus_tag="ID725_RS19485"
                     /old_locus_tag="pgaptmp_003897"
     CDS             7406..7801
                     /gene="ygdD"
                     /locus_tag="ID725_RS19485"
                     /old_locus_tag="pgaptmp_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGV7852388.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7794..8894
                     /gene="rlmM"
                     /locus_tag="ID725_RS19490"
                     /old_locus_tag="pgaptmp_003898"
     CDS             7794..8894
                     /gene="rlmM"
                     /locus_tag="ID725_RS19490"
                     /old_locus_tag="pgaptmp_003898"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGV7852389.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8938..9669)
                     /gene="fucR"
                     /locus_tag="ID725_RS19495"
                     /old_locus_tag="pgaptmp_003899"
     CDS             complement(8938..9669)
                     /gene="fucR"
                     /locus_tag="ID725_RS19495"
                     /old_locus_tag="pgaptmp_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGV7852390.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(9727..10149)
                     /gene="fucU"
                     /locus_tag="ID725_RS19500"
                     /old_locus_tag="pgaptmp_003900"
     CDS             complement(9727..10149)
                     /gene="fucU"
                     /locus_tag="ID725_RS19500"
                     /old_locus_tag="pgaptmp_003900"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGV7852391.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(10151..11569)
                     /gene="fucK"
                     /locus_tag="ID725_RS19505"
                     /old_locus_tag="pgaptmp_003901"
     CDS             complement(10151..11569)
                     /gene="fucK"
                     /locus_tag="ID725_RS19505"
                     /old_locus_tag="pgaptmp_003901"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGV7852392.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(11678..13453)
                     /gene="fucI"
                     /locus_tag="ID725_RS19510"
                     /old_locus_tag="pgaptmp_003902"
     CDS             complement(11678..13453)
                     /gene="fucI"
                     /locus_tag="ID725_RS19510"
                     /old_locus_tag="pgaptmp_003902"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGV7852393.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(13486..14802)
                     /gene="fucP"
                     /locus_tag="ID725_RS19515"
                     /old_locus_tag="pgaptmp_003903"
     CDS             complement(13486..14802)
                     /gene="fucP"
                     /locus_tag="ID725_RS19515"
                     /old_locus_tag="pgaptmp_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGV7852394.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3666 a         3719 c         3990 g         3698 t
ORIGIN      
        1 tatcagtaat ggcgtgaata taaaaagaat gtttaccgtt tattagatga cagattatgc
       61 gtctttcgct aaagtttccg gtcaaattag tcgtttactt gttacacagc ttagatttct
      121 tttgctcggg agaggcatgt caggatccaa cactgcaatc agccgtcgtc gtttactgca
      181 aggcgcgggt gccatgtggc tattgagcgt aagtcaggtc agtctggctg cggtcagcca
      241 ggtcgtggcg gtgcgcgtct ggcctgcgtc cagctacacc cgcgtgacgg tagaatcaaa
      301 tcgtcagctg aaatataagc agttcgcgtt gagtaacccg gaacgcgtgg tggtggatat
      361 cgaagatgta aacctgaact cggtactcaa ggggatggcg gcacaaattc gtgcagacga
      421 cccgttcatc aagtcggcgc gcgtcgggca atttgacccg caaaccgtac ggatggtttt
      481 tgaattaaag caaaacgtaa aaccgcagct gtttgccctt gcgccggtcg ccgggtttaa
      541 agagcgtctg gtgatggacc tctatccggc caatgcacag gatatgcagg acccgctgct
      601 ggcgctgctg gaggattaca acaaaggcga cctcgaaaag caggtgccgc cagcgcaaag
      661 tggtccacaa ccaggtaaag cagggcgcga tcgtccgatt gtcattatgc ttgaccctgg
      721 ccacggtggc gaagactccg gtgcggtggg gaaatacaaa acgcgcgaaa aagatgtggt
      781 attgcaaata gctcgccgtt tgcgctctct gatcgagaaa gagggcaata tgaaggtgta
      841 catgacgcgc aatgaagaca tcttcattcc gttgcaagtg cgcgtagcaa aagctcagaa
      901 acagcgtgct gacctgtttg tctctatcca tgccgacgcc tttaccagtc gccagccgag
      961 cggttcctcg gtgtttgcgc tctcaaccaa aggtgcaacc agtactgcgg caaaatatct
     1021 ggcacaaacc cagaacgcct ccgacttgat tggtggcgtg agcaaaagcg gtgaccgcta
     1081 tgtcgaccac accatgttcg atatggtgca gtcgctgacc attgctgaca gccttaagtt
     1141 tggtaaagcg gtgctgaata agctcggtaa aatcaacaag ctgcataaaa atcaagttga
     1201 acaggccggg tttgccgtac taaaggcacc agatattccc tccattctgg tcgaaacggc
     1261 gtttatcagt aacgttgagg aagagcgtaa actgaaaacg gcgaccttcc agcaggaagt
     1321 tgcggagtct attcttgcgg gaattaaagc gtattttgcc gatggggcga ccctggcgag
     1381 aaggggatga taaaaaggcg ctgaatggcg cttttttata ggagaccaga aacaaaaaaa
     1441 cacccgttag ggtgttcaat aattggttgc gggggccgga tttgaaccga cgaccttcgg
     1501 gttatgagcc cgacgagcta ccaggctgct ccaccccgcg tcaccgtact gctttacttc
     1561 atcaaatttt aattggttgc gggggccgga tttgaaccga cgaccttcgg gttatgagcc
     1621 cgacgagcta ccaggctgct ccaccccgcg tccgtggatg cgcactatac tccgctcgcg
     1681 ttttcatgca accttttttt taccccgatc acggatctgg catgattttg aacaaaaaca
     1741 aatcactaaa gcctattttt tgtgcaaaat ttgcgtcagg gagaggctgc tcatttcatt
     1801 aaacgcggtg gtttgttatc ttcgttgcgc cttatttttt aacctgaaga agagaacaat
     1861 gaaaggacgt tgggtaaagt accttcttat gggcacggtt gtggcaatgc ttgccgcctg
     1921 ctcttccaaa ccaaccgatc gcggacagca atataaagac gggaaattta cccagccttt
     1981 ctctctggtg aaccagccag atgccgttgg cgcgccgatt aacgccggtg attttgccga
     2041 gcaaattaac catatccgta attcgtcacc gcgtctgtat ggcaaccaga gtaatgttta
     2101 taacgcggtg caagagtggc tgcgcgcagg cggtgatacc cgcaatatgc gccagttcgg
     2161 cattgatgcc tggcagatgg aaggtgccga caactatggt aacgtgcagt ttacaggcta
     2221 ttacacgccg gtaattcagg cgcgccatac ccgccagggc gagttccagt atcctattta
     2281 ccgtatgccg ccaaaacgtg gtcgtctgcc gtctcgtgcg gagatctacg cgggtgcatt
     2341 gagcgataag tacatcctcg cttacagcaa ctctctgatg gataatttca ttatggatgt
     2401 gcagggtagt gggtatatcg actttggtga tggcagtccg cttaactttt tcagctatgc
     2461 agggaaaaac ggtcatgcct atcgcagcat tggtaaggtg ctgatcgacc gtggcgaagt
     2521 gaaaaaagaa gatatgtcga tgcaggcgat tcgtcattgg ggcgaaacac acagtgaagc
     2581 cgaggttcgc gagctgctgg aacagaaccc gtctttcgtt ttctttaaac cgcaatcttt
     2641 tgcaccggtg aaaggggcaa gtgcggtgcc gctggttggc cgtgcttctg ttgcctctga
     2701 tcgttccatt atcccaccag gtacaacctt gctggcagaa gtgccgctgc tggataataa
     2761 cggcaaattt aacggtcagt acgaattgcg tctgatggtg gcgttggatg tcggtggcgc
     2821 aatcaaaggc caacacttcg atatttatca agggatcggg ccggaagccg gacaccgcgc
     2881 aggttggtac aaccactatg gacgtgtctg ggtgctgaaa accgccccgg gcgcaggtaa
     2941 cgtctttagc ggctgatgtg atattctgta tgccgttttt acggatatga cagggtgagg
     3001 ggtaacctca catcctgatt attgactaag tgcgcgttgt tcatgccgga tgcggcgtga
     3061 acgccttatc cggcctacaa atcatgcaaa tccaaaatat tgcaggagtt atgtaggcct
     3121 gataagcgta gcgcatcagg catttatgcg tttagggtga gggataacct cgcccttttt
     3181 aattctgagg tgctatgtct gtggtaatta gtgatgcatg gcgtcagcgt tttggtggca
     3241 cagcgcgtct gtatggtgaa aaagcgttgc aactgtttgc tgacgcgcat atttgtgtgg
     3301 tcggtattgg cggtgtcggt tcctgggcgg cggaagcgct ggcgcgcacg gggattggcg
     3361 caatcacgct tatcgatatg gatgatgtgt gcgtcaccaa taccaatcgg caaattcatg
     3421 ccctgcgcga taacgttggg ctggcaaaag cggaagtcat ggcggaacgt attcgccaga
     3481 ttaactcgga gtgctgtgta acggtggtgg atgatttcgt gacgccggat aacgtagcgc
     3541 agcatatgag cgtgggttat tcgtacgtga ttgatgccat tgatagtgta cggcccaaag
     3601 cggcgctgat tgcttattgt cggcgcaata aaatcccgct ggtcacgacc ggtggcgcgg
     3661 gtgggcagat tgacccaacg cagattcagg ttaccgatct ggcgaaaacg attcaggacc
     3721 cgctggcggc gaagttgcgt gagcgcctga aaagcgattt tggcgtagtg aaaaacagta
     3781 aaggtaagct cggtgtggat tgcgtgttct ctaccgaagc attggtgtat ccgcagtcag
     3841 acggtacggt gtgtgcgatg aaagccacgg cggaaggacc aaagcggatg gattgtgcat
     3901 ctggatttgg cgcggcaacg atggtgaccg ccacctttgg ttttgttgcg gtttctcatg
     3961 cgctgaagaa gatgatggcg aaagcggcgc gtcaggggta aacaatcagg tcggatgcga
     4021 cgcaaacgtc ttatccgacc cggttctgtg cttaaacctg cttcgtcgca gcgataatcg
     4081 cctcgcttaa cgcatttaac ccctggctgc gtgaggcgct aagttgcgca cgcaatccca
     4141 gctcatcaaa caatgccagc ggcgactgtg cctgcaattc ggcggcggtt ttcccctcaa
     4201 cggcagtcaa cagcaccgcc aggaggccgc gcactatacg tccttcgctg tcgccaaaga
     4261 agtgcatttt accgttttca gccacggtat atcccagcca gacgcggttt tcgcatccgg
     4321 caatctcttt agcctgcgct tttaactcgt ctggcaaagc cggaagctgt ttccccagca
     4381 tgatcaactg gcgatattta tcttcccatt gcgtcaacgg tgcgaaggta ttgcgtaacg
     4441 tttctgcggt tacggttgtg ccgaacggat gtccggcgaa ttgcgggttc gtcattaatc
     4501 caccaataat tccagcgcgc ggtcaacggc attcaccagc gcatccacat cactctttgt
     4561 attatatggc gcaaaagagg cgcgcagtgt gccggtcacg cctaattctg ccagtagcgg
     4621 ctgagcgcaa tgttgcccgg cccgcaaggc aataccgtac tccgccagca gcgtcaccat
     4681 atcgctgtga tgaacgccag caaaatcaaa ggccagcagg ctggaatcct ggcagcggaa
     4741 tgaacgaaag cctggacgtt tcgccagcgc atcttccgcc agcgttgcta agctacggct
     4801 ccagttttcg gcctggttga tatcgtaatc tgccagccat tccagcgccg cgcttaatcc
     4861 tatgacacca gccacatttg gcgttcctgc ttccagtttc cacggcgcag attgagtcgt
     4921 gaagccgtca aaactcactt cgtgaaccat tttgccgccg cccagccagg gcgacatcgc
     4981 ttccagcagt tctgatttgc catacagcac gccgataccc gtcgggccat acagtttgtg
     5041 accagagaaa gcatagaaat cgatatccag ttgctgaaca tccgcgggga aatgcaccgc
     5101 cccctgagca ccatcaacta tcaccactat cccggctgaa tgagcaaagg taatcgctcg
     5161 cgccagatcc gggcaaccgc cagttacgtt cgacatctga cccaacgcca gaatccgact
     5221 acggggagta atcagttccg gcagcaaatc gacatctggc agtcgctgcg cattaagcgg
     5281 caatttgacc actttggctc cagtttgttg ggcgaccatc agccagggga cgaggttggc
     5341 gtggtgttct gccacgctga caataatctc atcgcccggt tgcagacgcg gacgcgcata
     5401 gcattgtgcc accatgttga tggattcagt ggtgccgcgc gtccagacga tagttttatc
     5461 atccggtgca ttcagtaatt gcgccacttt ctctcgtgca gcttcataac gcgcggtcag
     5521 gcgttgggct tcggcaaact ggctgcgatg gacgtttccg gcgctcagac tgtaaaactg
     5581 tcgggtggct tcaaccacgg cttcaggttt aagcgcggtc gcggcgctgt cgagatagac
     5641 gcccgcatcc tgtagagcgg gaaactgggc gcgaaactgc gcgggattaa aaacgttcat
     5701 ggtactcctc ggcttgatgc accgatcgtg acgcaaaatt cactttgata caaggagagt
     5761 gctaaccatc agaattatct ggatatcctg gcgaagccgc gtcagtaaca tatgctgaat
     5821 agtgttaggc gttaatttta gcctgttaat caatactatt aagatttatt aatcgcttta
     5881 aggagaagat gatgaaaaag actgccgcaa ttatttctgc ctgtatgctg acttttgccc
     5941 tgagcgcctg ttccggttcg aactatgtga tgcacaccaa tgacggacgt accatcgtct
     6001 ctgacggcaa accacagact gataacgata ccggtatgat ttcgtataaa gacgctaatg
     6061 gcaacaaaca gcagatcaac cgtaccgacg tgaaagagat ggtcgaactg gatcagtaat
     6121 tcgcgatcgc aaggtaaaaa aaagcaccgc aattaggcgg tgctacatta atcactatgg
     6181 acagacaggg taaatgtaca ggaagtgaaa aaaggtagct ttgctaccat ggtctgaatc
     6241 gcagaccaat tgcaaacaca acaacacaac atcacaaccg taagccaaaa gttcaccaga
     6301 acacgcattc cgataaaact tttcgttccg gctcaggaag tgccgccact ataggtattt
     6361 gctggtagaa gctcaacgga caatttataa tggctcagat taaaaaaact aataggttac
     6421 acagtgtgat ctaattgtta aattcattta acatcaaagt ttaaaagcca tgtctaaacg
     6481 attaccaccg ctaaatgcct tacgagtttt tgatgccgca gcacgccatt taagtttcac
     6541 tcgcgcagca gaagagcttt ttgtgaccca ggccgcagta agtcatcaaa tcaagtctct
     6601 tgaggatttt ttggggctaa aactgttccg ccgccgtaat cgttcactcc tgctgaccga
     6661 ggaagggcaa agctatttcc tcgatatcaa agagatattt tcgcaattaa ccgaagcgac
     6721 gcgtaaactc caggcccgta gcgccaaggg ggcgttgacg gtcagtttac tccccagttt
     6781 cgccattcat tggttggttc cgcgactttc cagctttaat tcagcttatc cgggaattga
     6841 cgttcgaatc caggcggttg atcgtcagga agataagctg gcggatgatg ttgatgtggc
     6901 gatattttat ggtcggggca actggccggg gctacgggtg gaaaaactgt acgccgaata
     6961 tttattgccg gtgtgttcgc cgctactgct gactggcgaa aaacccttga agaccccgga
     7021 agatctggct aaacatacgt tattacatga tgcgtcacgc cgtgactggc agacatatac
     7081 tcgtcaattg gggttaaatc atatcaacgt tcagcaaggg ccaattttta gccatagcgc
     7141 catggtgctg caagcggcta ttcacgggca gggagtggcg ctggcaaata acgtgatggc
     7201 gcaatctgaa attgaagccg gacgtcttgt ttgcccgttt aatgatgttc tggtcagtaa
     7261 aaacgctttt tatctggttt gtcatgacag ccaggcagaa ctgggtaaaa tagccgcctt
     7321 tcgccaatgg atcctggcga aagccgctgc tgaacaagaa aaattccgct ttcgttatga
     7381 acaataattt acgtagggta cgaccatgac cagccgtttt atgctgattt tcgccgccat
     7441 tagcggcttc atttttgtgg cactgggcgc ttttggcgcg catgtgttaa gtaaaaccat
     7501 gggggccgtt gagatgggct ggatccagac cggcctcgaa taccaggcgt ttcatacgct
     7561 ggcgatctta ggtctggcgg tggcaatgca gcgtcgcatc agtatctggt tttactggag
     7621 tagcgttttc ctcgcgttag gcacggtgct gttcagcggc agcctttatt gcctggcact
     7681 gtcccatctg cgtttgtggg cgtttgtcac tccggttggc ggcgtgagct tccttgcggg
     7741 ctgggcgtta atgttagttg gtgctatccg tttaaagcgc aagggcgtaa gtcatgaata
     7801 aggttgtatt gctgtgccgt ccgggctttg aaaaagagtg cgccgcagaa attaccgata
     7861 aagccggcca gcgggagatt ttcggttttg cccgcgtgaa agagaatgcg ggttatgtca
     7921 tttatgaatg ttatcaacct gatgatggcg ataagttaat ccgtgagctg ccgttcagtt
     7981 cattaatttt tgcccgccag tggtttgtgg tgggggaact cttgcagcat ttgccgccag
     8041 aagatcgtat tacccccatt gtcggcatgt tgcagggcgt agtagagaag ggcggtgaac
     8101 tgcgtgttga agttgccgat accaacgaaa gcaaagagtt gctgaaattc tgccgtaaat
     8161 tcaccgtgcc gctacgcgct gccttgcgcg atgccggggt gctggcgaac tatgaaacgc
     8221 cgaagcgtcc ggttgtgcat gtattcttta ttgcaccagg ctgctgctat accggttact
     8281 catacagcaa caataattcg ccgttctata tgggcattcc gcgcctgaaa tttccggcag
     8341 atgcgccgag tcgttccacg ctcaaactgg aagaggcatt tcatgtgttt attcctgcgg
     8401 atgagtggga tgaacgcctg gcgaacggga tgtgggcggt ggatttaggc gcttgccctg
     8461 gcggctggac ctaccaactg gtgaagcgca acatgtgggt ttattccgtc gacaacggcc
     8521 cgatggcgca aagtctgatg gataccggac aggtgacgtg gctgcgggaa gacggtttca
     8581 aattccgtcc gacgcgcagc aatatctcct ggatggtatg cgatatggtt gaaaaaccgg
     8641 cgaaagttgc ggcattgatg gcgcagtggc tggttaatgg ctggtgtcgt gaaaccattt
     8701 tcaacctcaa actgccgatg aaaaaacgct acgaagaagt gtcacacaat ctggcgtata
     8761 ttcaggcaca gcttgatgaa catggcataa atgctcagat tcaggcgcgg cagttgtatc
     8821 acgatcgcga agaagtgacg gtgcacgtcc gccgtatctg ggctgcggtg ggtggtcgtc
     8881 gcgacgagcg ataacaggca aggccggggc gtcgtctccg gcctgctacc ctttccgtca
     8941 gcctgttacc aaagaagttg caacctggcg ttcatcagaa ataatgtgcg ttacctcatc
     9001 cagatgcccg atgcgggctt cccctgaacg attaaattta cttttatcaa tcaataacaa
     9061 cgattgcgcg gcgcgtttta atagcattga tttgtaatca gcgttgatag cattggagtc
     9121 ccacagtgcg ccgctgctat cgatcccttc acaagaaaaa ataaacagat cgatttccag
     9181 cgatttaagt tgggaaatca gcgagggatt gacgtaacag ccatatttgc gctcaagtgt
     9241 gccgccggaa ctgatcagtt gaatgcgttc gcgtttaccg agttcatggc aaatcggatg
     9301 gctattggtg aagacctgaa tgttgatgtc aggcaactgg cgtgccagat accagcaagt
     9361 tgaactggcg tctaaggcta tcaccatccc ttcttcaatc cacgcgagcg cctcgcgcgc
     9421 gatatctgct ttatgcgcat aatggctttt cagcctgatg tgaaagggat cgccactgtc
     9481 ttgattttga cggtggatat atttagcgcg tccatgattg cgcagaattt taccctgcgt
     9541 ctgtaattca ttgagatcgc gacgaatggt ttctttactg acctttagct gttcagagag
     9601 agcttccgtg gtcaggctgg tatggttcag cagcaggtcg actatcgctt gctggcgtgc
     9661 cgctttcata tttttcaccc catcattcga taaccgcccg cgtcatgcgg gcgtaccggt
     9721 atgagattac ggtgttaccc ctttttttaa aagaatattc ccgtacttcg ctcgttcgcc
     9781 tgtgataacg atcgcaaagg ctttttgcgc ccgttcataa aacgcaaaac gattgatgcg
     9841 gatgatgtca ggacacgggg cttgtagtga aagcgcatta cggtaacgtc gttctacttc
     9901 aggatcgaga gtgtcacctt ctaccgctgc catcatcacc agcggcggtg cataactgtc
     9961 cagctcaaat aacgggataa tcgcctggag caagtcgctc accaacaggc catcagcgcg
    10021 gatcacctgc ggtcccatcg aatgggcggg aaagtgagca tcggaaaaaa taatttcatc
    10081 tccatgtccc atctctgcca gcactttcaa tagttcggga gaaattaacg gcgaaattgt
    10141 tttcagcatt tcacacttcc tctataaatt caggttcagt ttgcgggtag aaataacggt
    10201 actgataatg aatctgtgca cgggcttctt ccgggctgtt aaattcccct acgccatacc
    10261 aaccgaacag cgcagctcct gcgacggtcg tttcggcgtc gtcgagaact tttaacggaa
    10321 tatcaagcat attggcttta atctgattcc acaatgtgtt gcgacttcct ccaccgacta
    10381 acaataattc agaggcctta aagtgcccga ttttttccag catctgtaga ttgcgctgta
    10441 attgcgcagt taacccttcc agcgccgcgc gatagaaatg cccccgcgtg gtattaagcg
    10501 tcactccttg ccagccagcg ttctgacacg acaataaatc acactgcatt ttcacgccat
    10561 ccgcgccagg cgcgatcaga cgagcctctt caatcaacat ttgccagggt gtttcagccg
    10621 tccagaacag ttttctcacc cattccagca caccggatgc cagccattgc atacctgggt
    10681 tatacaaccc tgcctggcta tccagttcgc aggtggaacc ggcgtactga cttaacagcg
    10741 aagtatcaac ctgggcgctg cgaaccatta aaatttccca tgtaccggaa gagagcacgg
    10801 gttcattttg ttcagcacca gcgccaaaaa gggcgaactg ggtatcgtga cctgcggaaa
    10861 tcaccggtat gccaacgggt aagccgagca ttgctgcggc gctgttctgt agcgtaccaa
    10921 tctgttcacc cgcttccacc agacgaggga agagtcggcg tggaataccg gtggcttgta
    10981 aaatttgcgg actgaaatcg cgttgctgga tatccagcat ctggctggtt ccggccatcg
    11041 tgatatcagt agtgaattcg ccggttaaac ggtggttaat cagcgacgaa ataaagagcc
    11101 aggcgtgcgc gcgttccagc agttgtggat gattttcttt caaccacacc aacttatata
    11161 acgtattgaa actaaaggct ccgacgccag aaatagcctg caaccgctgt gcggagatta
    11221 accgttcaat attgtccata accgctgctg ttcgcggaca tttccagcta atgatcggat
    11281 agagcagatt gccttgctta tctaccagag cgccatccac accaaaggtg gtgacggcga
    11341 taccgcggat gtggcattca gtcagttcac tattgatttg ccgacagcaa tcagcaaagc
    11401 gttgcaaaat ggcgtctaaa gaccactggt gccaggtgtt gttttccatc gcgatatcgc
    11461 tggcattagg cgttgaggcg cgggcaacaa tttttccctg ccgattaacc gcgatggccc
    11521 tgacattggt cgcgccacag tcgagtacca ggatcacttc ttgtttcatg atcgctcctg
    11581 caatatagcc ggataacatt gcttatccgg ctaaccactc ttgcttgttc aggcccggca
    11641 gccggagcga ccgggcatca catcagggag taatgtatta acgcttgtac aacggaccgt
    11701 agttctggca agcgcggtaa tcctggcctt caatatccat gccgtgcgca gcccaggcag
    11761 aaggacgata cactttggtc tcttcaacgt tgtgcataca taccgggata cgcagcatgg
    11821 aggcgagagt gataaagtct gcgccaacgt ggccgatggt cagaacccca tggttagcgc
    11881 cccagttcgc cattaccgag tacacatccg taaacggccc tttaccggtg aggcgcggtg
    11941 caaaccaggt ggttggccag gttgagttgg tgcgtttgtt gaggatgtca tgcacatcct
    12001 tcggcaattc cacgctccag ccttccgcga tttgcagtac cggtcccagg cctttgatga
    12061 tgttgacacg agtcatggtg aacgggacgc cgccttcggt aaggaagcgg gaagagtaac
    12121 cgccgccacg gaagtattcg tggatcgccg ggcaccattc ggtagcggcg aggcaagcgt
    12181 cagcctcttg ctgagagatt tcccagtgcg gcttcatcgt cgggttacct tcgctgtcgc
    12241 gttgtttaca ggaaccgtcc agcgcagcag aaccggagtt gatcaaatgg atgatgccgt
    12301 gttctgccag tccatccagt ttatgccccg ttacacgctc aattgcttct ggtgaccagt
    12361 aggtacgcac atcggcaaat acctgagcgg tgccggtgag ctggtgaccc attagcattg
    12421 ccacgccgtt aagactgtcg ttttcggtcg ccacgacaaa gggttcgcgc acgccattcc
    12481 agtcaaatga actgttgagg atcgcttcgg cggtgtcacc attgggatat tgatcggtcc
    12541 agtgacgttg cccctggaag cccgcagcga tggcgttgta gccaagtgat tcttccacgc
    12601 gaccaatatc ggccagtttg ctgttgcctt gcatcatgtc gcggatacac atcgccatca
    12661 gtaaactttc gcgcagaact gcgcggcttt gctcggcatt gcgttgatac tgtttgttat
    12721 tttcatcttc gccatagcgg aagtttttat cagcccaggc cagtgccatt tccagttcgg
    12781 cttcgtcgta aatcttctga tcgatacggc gacgcagttc ggtcatatcc accgcctgga
    12841 ctttcattcc cagccaggat tcaaagaagt tgtgatcaac aatagaaccg gcgataccca
    12901 tcgaaacgcc gcccagcgac agatagcttt tacctttcat gctggcgacg gccaaaccgg
    12961 cgcgggcaaa gcgcagcagt ttttcttcaa catcggcagg aatcgatgtg tcatcggcat
    13021 cctgaacgtc atgaccgtaa atggagaatg ccgggatgcc tttctggctg tgagctgcca
    13081 gagccgctgc caggtaaaca gcgccgggac gttcagtgcc gttaaagccc caaatggctt
    13141 tcgggcgggt tggatccatg tcgatggttt cactgccata gcaccagcaa ggcgttaccg
    13201 taatggtgag gcctacattc tggctgctga atttttcttc gcaagcagcg gcttcagcca
    13261 tacccgcgat acaggtatcg gaaatgacac actcgacggc agctccgcag gcatggcgca
    13321 gtttctcggt cagcagtgcg gccgtagctt tcgccatatt cattgtttgt tcttcaagcg
    13381 actcacgaac acccatgcga cgaccgtcaa taaccgggcg gataccaatt ttcggtaagc
    13441 tgattttgtt cattacagat tcctcacttt attcggaaaa tatgttcagt tagttgccgt
    13501 ttgagaacgg aaacgggcaa agataaagat gaccgcgaag cagagtgcgg ggatcagttc
    13561 agcagtgggg atgttgcccg ccgcgtcact gacaaaaccc atgaccggag tgacaatacc
    13621 gccgccaatg atggtcataa cgatgaagga cgaaccgtat ttggtgtcct ggccgagatt
    13681 cttaatgccc agcgagaaga ttgttgggta ctgaatcgac ataaaggcgc tgcataaagt
    13741 cagggctatt aagcccacat gactgccagc gaaggctgag atcaggcaca gtgccatagc
    13801 gattaatgcg taggcggcca ggactttgtg tggtgcgaag cgactgatga gccaggtacc
    13861 ggtgaaacga ccaataaaga agcacaccat ggttccggtt aaatagttag cggcaaagcc
    13921 tgcagtcatg cctggaattt cttctacagc gtagcgaatc aaatagctcc agcaggccgt
    13981 ttgtgcgcca acgtagcaga attgcgctaa tactgcccag cgccagtggc gaatacgcgc
    14041 caggcgagaa agcgatgcgg agaacgatcc ttgtttggcg tcactgtgat tatcactctg
    14101 caatgccggg aatttcgtca gcatgatcag cagggcgacc agtaacacga tagccacgat
    14161 gatcatataa ggtgtctgta ccgataatac caggctgtgt ttatacgcac tcaattgctc
    14221 tggagacatt ttatcgagaa cgtcttgcga ttgatgtggc acgttagaca aaataagact
    14281 ttgcccaaag acaaccgcga taattgcgcc aaatgagtta aatgtttgcg caagatttaa
    14341 gcggaagtga ccactgcttt ccggccctaa taccgtaaca aaagggtttg cggcagtttc
    14401 cagacaacct aatccggctg caataataaa taggccaact aaaaataagg tgtagttcat
    14461 tatttctgcg gcgggccaga ataatgcagc acccaaggca tataaaaata atccggtaat
    14521 aattcctgct ttataactga gttttttcat caatatccca gcaggaattg ggataatgaa
    14581 ataaccaaag taaaaggccg attggatcag gccagcctgg aaatttgtca gtgtaaaagc
    14641 ctgctggaat tgaggtaata aaatgtcgtt aaggttattg gctaccgccc aaagaaaaaa
    14701 cagtgagcac agcagcgcga atggaataat gtaacttctg ctttgccctg catctttatc
    14761 taccgcacgg taactctgcg tttgtattga tgtgtttccc atagcatcct cttaggttca
    14821 gaagcttaag cacgtaatcg gttatgtttt caaaagtgca aaactagcga gttcgggctt
    14881 catatcagtg aatatttgcc tgtctgaaat gaactcatgg attcattttc aaataaaaaa
    14941 cgctctgtaa tatgacggcg gtcacactta ttagctggaa ggtgacttta tgtgactacc
    15001 atcactttaa taatcgaaat ttcatgcccg tttcagaaaa taaatcataa aaaacataaa
    15061 ccagatgacc gta
//
LOCUS       DABSYT020000106        15057 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.106, whole genome shotgun sequence.
ACCESSION   DABSYT020000106 DABSYT020000000
VERSION     DABSYT020000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.106"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            61..417
                     /gene="yhaI"
                     /locus_tag="ID725_RS19520"
                     /old_locus_tag="pgaptmp_003904"
     CDS             61..417
                     /gene="yhaI"
                     /locus_tag="ID725_RS19520"
                     /old_locus_tag="pgaptmp_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGV7852395.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(468..1364)
                     /gene="yhaJ"
                     /locus_tag="ID725_RS19525"
                     /old_locus_tag="pgaptmp_003905"
     CDS             complement(468..1364)
                     /gene="yhaJ"
                     /locus_tag="ID725_RS19525"
                     /old_locus_tag="pgaptmp_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGV7852396.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            1469..2170
                     /gene="yhaK"
                     /locus_tag="ID725_RS19530"
                     /old_locus_tag="pgaptmp_003906"
     CDS             1469..2170
                     /gene="yhaK"
                     /locus_tag="ID725_RS19530"
                     /old_locus_tag="pgaptmp_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGV7852397.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2193..2357
                     /gene="yhaL"
                     /locus_tag="ID725_RS19535"
                     /old_locus_tag="pgaptmp_003907"
     CDS             2193..2357
                     /gene="yhaL"
                     /locus_tag="ID725_RS19535"
                     /old_locus_tag="pgaptmp_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGV7852398.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2491..3801)
                     /gene="cyuA"
                     /locus_tag="ID725_RS19540"
                     /old_locus_tag="pgaptmp_003908"
     CDS             complement(2491..3801)
                     /gene="cyuA"
                     /locus_tag="ID725_RS19540"
                     /old_locus_tag="pgaptmp_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGV7852399.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3829..5160)
                     /gene="cyuP"
                     /locus_tag="ID725_RS19545"
                     /old_locus_tag="pgaptmp_003909"
     CDS             complement(3829..5160)
                     /gene="cyuP"
                     /locus_tag="ID725_RS19545"
                     /old_locus_tag="pgaptmp_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGV7852400.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5435..6799)
                     /gene="tdcG"
                     /locus_tag="ID725_RS19550"
                     /old_locus_tag="pgaptmp_003910"
     CDS             complement(5435..6799)
                     /gene="tdcG"
                     /locus_tag="ID725_RS19550"
                     /old_locus_tag="pgaptmp_003910"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGV7852401.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6871..7260)
                     /gene="tdcF"
                     /locus_tag="ID725_RS19555"
                     /old_locus_tag="pgaptmp_003911"
     CDS             complement(6871..7260)
                     /gene="tdcF"
                     /locus_tag="ID725_RS19555"
                     /old_locus_tag="pgaptmp_003911"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGV7852402.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7274..9568)
                     /gene="tdcE"
                     /locus_tag="ID725_RS19560"
                     /old_locus_tag="pgaptmp_003912"
     CDS             complement(7274..9568)
                     /gene="tdcE"
                     /locus_tag="ID725_RS19560"
                     /old_locus_tag="pgaptmp_003912"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGV7852403.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9602..10810)
                     /gene="tdcD"
                     /locus_tag="ID725_RS19565"
                     /old_locus_tag="pgaptmp_003913"
     CDS             complement(9602..10810)
                     /gene="tdcD"
                     /locus_tag="ID725_RS19565"
                     /old_locus_tag="pgaptmp_003913"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGV7852404.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10836..12167)
                     /gene="tdcC"
                     /locus_tag="ID725_RS19570"
                     /old_locus_tag="pgaptmp_003914"
     CDS             complement(10836..12167)
                     /gene="tdcC"
                     /locus_tag="ID725_RS19570"
                     /old_locus_tag="pgaptmp_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGV7852405.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12189..13178)
                     /gene="tdcB"
                     /locus_tag="ID725_RS19575"
                     /old_locus_tag="pgaptmp_003915"
     CDS             complement(12189..13178)
                     /gene="tdcB"
                     /locus_tag="ID725_RS19575"
                     /old_locus_tag="pgaptmp_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGV7852406.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13277..14215)
                     /gene="tdcA"
                     /locus_tag="ID725_RS19580"
                     /old_locus_tag="pgaptmp_003916"
     CDS             complement(13277..14215)
                     /gene="tdcA"
                     /locus_tag="ID725_RS19580"
                     /old_locus_tag="pgaptmp_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGV7852407.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14530..14748
                     /gene="tdcR"
                     /locus_tag="ID725_RS19585"
                     /old_locus_tag="pgaptmp_003917"
     CDS             14530..14748
                     /gene="tdcR"
                     /locus_tag="ID725_RS19585"
                     /old_locus_tag="pgaptmp_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HGV7852408.1"
                     /translation="MSKFSNFIINKPFSAINNAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT         3941 a         3975 c         3478 g         3663 t
ORIGIN      
        1 atagcattta cacaataccc ggaatagact ttcgtcactt ttaattaaag ggatgttttt
       61 atgcagtggt atttatccgt attaaaaaat tatgttggtt tctctggtcg cgcacgtcgt
      121 aaagagtact ggatgtttac tctgattaac gccatcgtcg gcgctattat caatgtcatt
      181 caattgattt taggtctgga gcttccatat ctgtctatgc tttatttgct ggcaaccttc
      241 cttcccgtcc ttgcgcttgc tatccgtcgc ttacacgata ccgaccgttc aggtgcctgg
      301 gccttgctct tttttgttcc gtttattggc tggctcgtgc ttctggtttt tttctgcaca
      361 gaaggtactt ctggcagcaa tcgttacgga aacgatccga agtttggttc aaattaatct
      421 tagaattggg gcgatatttc gccccttttt attaacaata ataaatatta ttttccgtta
      481 aaaagtttgg gaatttcccg cagacaccag gatttcgctt cccccatact gtcacgacgc
      541 caggccataa taatatcgat ctcgctggtc gattccgggc tgacgacacg caaccgccct
      601 tccgcaatat ctttttcgac catcggatac ggcatcgtcg ccacgccaag ccccgccagt
      661 aatgcctgac gtttatcttc aatcgtgctc accgttaagc gcggctgttt gtccagcagc
      721 tgtacagtca acaccgggcg ctcacgagcg gtatccgcca ccgcaattcc acgatatttc
      781 acacgcgtca cttcagataa cggttccggc tcctgatgaa tcgggtgatc aggcgcggca
      841 acgtagacgt tcattaacgt atagagcttg cgcgagttga tctccgacga ggaacgaaaa
      901 tgcatatccg gcgcgataac aatatccgcc cgcccctgct ccagccgttc ccacgccccc
      961 gccagcactt ctgtgatgat tgccagttgg gtattggctt ttgccgccag tttgtcgatt
     1021 aacgggaaaa aggcaggtgt cggtaccagc gcttcggtca caatggtgag atgcgtttcc
     1081 caaccgcgcg cgagagcttc cgcatcagta gtcagtttat ctgcggcttc cagcaaaacg
     1141 cgccctcgct ccaacaacat cctcccgaca ttggtgaatt tggtgcgatg gcccgagcgg
     1201 tcaaacagca ccacatccag ctcttcttcc agtttttgca tggtgtagct aagtgcggaa
     1261 ggcacgcgtc ccagctcatc cgctgccgcc gcaaaactgc cccggcgatc gatcgcatcc
     1321 ataacccgta gtgcttccag cgttaatgcc ctttctttgg ccatttcgtt ctcattcagg
     1381 aaatttgaac ataccgggca gaatatctgg ctaacaatgt agcgtccagc cccttaccat
     1441 aaaaggaagt aaagagaggt caagaattat gattactacc cgaactgcca ggcagtgtgg
     1501 acaagcagac tacggatggt tgcaggcccg gtatactttt tcctttggac actacttcga
     1561 cccgaaattg ttaggctatg cctccctgcg tgtgcttaac caggaagtgc tggccccagg
     1621 tgccgccttt cagccgcgaa cctatcccaa agtcgatatt ttaaatgtga ttctggatgg
     1681 agaagcagag tatcgcgaca gcgaaggcaa tcatgttcag gccagcgccg gtgaggcgtt
     1741 gctgctctct acccagccgg gtgtcagcta cagcgaacac aatctcagca aagacaaacc
     1801 gttaacacga atgcagcttt ggctggacgc ctgcccgcag cgagagaatc cgctgattca
     1861 aaaactggcg cttaatatgg gcaagcagca tttaatcgcc tcgccagagg gtgcgatggg
     1921 aagcctgcaa ttacgccagc aagtgtggct gcaccatatc gtgctcgaca aaggcgaaag
     1981 tgcgaatttc cagttgcatg ggccacgcac gtatttgcaa tcgattcacg ggaaatttca
     2041 tgcgcttacg catcatgaag agaaagcggc gctgacctgc ggtgatgggg cgtttattcg
     2101 tgacgaggct aacattacgc tggttgccga ttccccactg cgcgctttgc tgatagattt
     2161 gcctgtctag ttgtttttac agggagatga tgatgagtaa aaaatcggcc aaaaagcgcc
     2221 agccagtgaa gcccgtggtg gcgaaagaac ctgctcgcac cgccaaaaat tttggctatg
     2281 aagagatgtt gagcgagctg gaagccatcg tcgcggatgc tgaaacgcgt ttagccgagg
     2341 atgaagctac cgcgtaaaga caggttgccg gatgcggcga aaacgcctta tccggcctac
     2401 aaaatccaac aacattaacg aattaaacaa cccgtaggcc ggataagatg cgccagcatc
     2461 gcatccggca cgatacccaa aacctggcgt ttatctggcc ttgctcgcca taatctcgat
     2521 aatctgccga tccgtttgct gcatcgaatg gcttgctaac gcacacaggt tggcaatcga
     2581 ctgctcaaca tcatgcgcca cgatcccttc attgccggtc acggcggtat catccagcgc
     2641 cattaacacc gctttccacg cagccgaagc actggtcgaa accttcatcg cgcagctgtt
     2701 cgacgcacca tcgcaaatca tgccgctgac atcgccgatc atactgctga tcgccatcga
     2761 gatggtttca taacgcccat ccaccagcca tgccatcccg gcggcggccc ccattgctgc
     2821 ggtcgttgcg gcacacagcg cagacaaacg cggtaactgg ttatggatgt aaattgcgct
     2881 caaatgagaa agcatcagcg cacgcgccag ccgttcatca tccgctccga agtgttctgc
     2941 taccaccacc acgggcattg ttgcggtgat cccctggtta cccgagccgg agttactcat
     3001 tgccggaagc gtggcgccgc ccatacgcgc atcggatgcc gcgctggtac gaatcacaat
     3061 ggatgaagag agatctttcg ccagcaaacc gcgctcgcac tgtttttcca gcgtcgcgcc
     3121 aatatgcagc ccccacttac cgctcaaacc ttcctgcgat aacgcacaat ttagcttcgc
     3181 ggaatcgaga ataaagcgga tcgccgcaaa cgggacttca ttgacgaact tcaggatctc
     3241 agccagcgtc gttctggaaa gcaccgatag cggagactct tgctcgccct ctgccacaca
     3301 cgcctgctgg gtaaacacca caccatcgtg cgtctcaata tgcacaatgt tggtatgccc
     3361 gccgacgata gtgacacacg cccacttctc accgttccag actttggcgc gtgagaagag
     3421 gatttcattg caaggttcct ggatcttaac ggagactttc cccgccgcca gcagtgcttt
     3481 ggcatcggca attgcctgcg cagttgcgtc tttcagcact tccagcccgg cgttggcatt
     3541 tccacctaac gcccccagcg ccgccgcaat cggcagcccc accattcccg tgccgggaac
     3601 ggtgacgccc agaccgttct tcatcagatt tggcgaaacc caggcttcta cacgttcaac
     3661 cggaccttcc agttctgccg cagcaaccgc cgccgccagc gccagtgaaa tcggttcagt
     3721 acatcccagc gccggtttta cttcctcctg aacggcgagg atgtaacgct gccataacgg
     3781 atttaaagtc gaatcaaaca tattaaaaac cttaaaattt caggtaaatc aagaaaatgc
     3841 caggaacgga gaaacacaaa gcaacaaacc agtgacgata atcaggtaca gagacatccc
     3901 tttgtatttg tgcaatgccg gtactttgta aaccagccac gccgggatca ggcaccctac
     3961 caagccgaaa atcgggctac agatagaggt gaagctcaac accggtgcgt tcagtacgat
     4021 ggcgctccag gccagcaaaa tggcgaaaat catgatgccg cgctgaacaa gattttcgtt
     4081 aatcttctcg gcaggcatct tgcgacgcag gatgttcatt acgatccctt gcgttgcttc
     4141 gcgaaaacct aaatagacgc caaagaatgc agtcatgaca gcaaagatat tgagaatgac
     4201 gctgaccact ttcacccacg ctgcgccgtc accgctaata aactgtgcgg caatcgccag
     4261 cgcggaaata ttctgctcgt aggcttttac cgcttcgtca tgtcccatcg ccagcgtgaa
     4321 cgacacggcg tagaaaaaga cgatgataaa caaaatgcca aacgcgatat tcattgcccg
     4381 caatgcttta tgccgcgcta cttcaatcga tttttcccgc gagcgataag agatcaccat
     4441 cgggcttaac gtctggataa acagaatcga cgtcagggta aacggcagcg taataatagc
     4501 gtttttcacc agcagcccca gcggcggtag cgaaccgacg ttatacagat gccacattcc
     4561 caccatcgac acgcccagcg ccgcgaccac cagcagcttg gtcagcacca tgccagtcga
     4621 aattttgaat aacaatttct cgccgcgtga agagatcgcc accagaatgc aaatcagtac
     4681 cagaccataa aagggactgt ctgacagcaa cccttccgtc acgccgaagg tatgcaggta
     4741 ggaagcacta tcgttggtga tggcggtgga atagacgaac atccaaatca ccagcattac
     4801 gaaatagagt gcacctaaca ggatgcccca gtttttacct aaataaccgc taatgacgct
     4861 cgggtaatct ttacattctg gtgattctgc cagcgtatta ataaacaacc gctgaaacag
     4921 atacattgcc gggtaaccaa tcaccgatga gagcaaaaat acccacaatc ccatcaaacc
     4981 gacctgcacc gggagaaaaa caatccccgc gccaatcgcc atcccgatac tcataatcac
     5041 ccagccggtg tcagtactgt cgaatttgat cgcttctcgc cactcgctct cactcattcc
     5101 agcacgaccc gccggggtcg aagcgtctgc aatgacgcct ttattcgatg caatttccat
     5161 aatttctcgc tcaatatttt gtagggctta ttattttttt ccgagccgca tcaaggcgat
     5221 atgcggtcta cgtttttgca ggcgagttat tagaataaag aaatgatacg cgcgaggtca
     5281 gagaaaatct tcacaatcaa gactttgaaa taacggagat ggataagaat tttctactaa
     5341 attaatcgca agagaatatt cactctctat ttgcggcaga tcacaaaaaa aaggtgcaca
     5401 tttgtgcacc caaggatgaa agctgacagc aatgtcagcc gcagaccact ttaatggcca
     5461 gtccgccgcg tgatgtttcg cggtatttat cgttcatatc tttgccggtt tcatacatcg
     5521 tctcgatcac tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc gccatccgcg
     5581 cggcgtttac tgctttcacg gcattaatgg cattacgttc aatgcacggg atttgtacct
     5641 gtccggcaac cggatcgcag gtcagtccaa ggttatgctc catcgcgatt tccgccgcat
     5701 tgcatacctg cgccggactg ccgcccagta gttcagttaa ccctgccgcc gccattgaac
     5761 aggccacgcc aatctccccc tgacagccga cctccgcgcc agagatggag gcgttcattt
     5821 tatacagcgc gccaatagcc ccggctgcaa gaaaatagcg ggcaattgac cgctcgttta
     5881 ccggacgacg gaacttatcg taataagcca gcactgccgg aataatgccg cacgcaccgt
     5941 tagtcggtgc cgttaccacg cgcccgccag ctgcgttttc ttcactaacc gccagcgcgt
     6001 acatgttgat ccagtcgatg acattcatcg gatcgttaga gatgttatcg ctggaaacca
     6061 gctgacgacg cagcgctacg gcacggcgcg gcacattgag cggaccaggc agcacgcctt
     6121 cagtgttcat cccgcgttca ataccgtcat gcatcacttg ccagatacgg gcaaaaccgg
     6181 cgtcaatttc cgctttactg cgcagcgcta gctcgttgtg catcatcagg ccagaaatag
     6241 acaggccgtt gtaatcacac attttcagca gttcacctgc tgagtggaaa tcgtaaggta
     6301 cgggtgtttc gacatcgtgc gacaggccga agtgttcttc ttcgacaata aacccgccgc
     6361 cgacagagta ataggtttta cttaatagct cttcctgtgc cttccaggca gtgatccgca
     6421 ttccgttctc atggcgaggc aacatttcgg gatggaagat aatgttcttt gctacaggaa
     6481 aatcaacaat atgcgcacca gatgccactg gcagccgccc gctgcgcgtt actaactcta
     6541 taaatgcagg gatctcatca atgacaacat cctgcggact gtttcctgcc agtcccatga
     6601 tgatggcgac atccgtggca tggcctttgc ccgtcagtga caacgacccg tacagatcga
     6661 ccacaatatg gctcgtcgcg gttaataagc cgctactttc cagccgatca ataaaacttt
     6721 ttccggcatt cattggcccc acggtatgcg aactggaggg accaatccca attttgaaaa
     6781 tatcgaatgc actaatcata tccacaccct cggattgccg ttcagtgaag tggagcggaa
     6841 cgaccttacg accgtcccgc tcacgaggct ttacgcacta cgtactgcga tagcttcaat
     6901 ttccagcttc acatctttcg gcaaacgcgc gacctgtaca cagctccggg tcgggtaggt
     6961 cgcctgatgc tcatcgaaga attgcttata gacttcgttg atggtggcaa aatcattcag
     7021 atcggtgatg aacacggtca tcttgatgat atcgcccacg ctcagcccgg cagcaaccac
     7081 gatcgctttg acgttttcga ggcttaaacg cgcctgatct tgcacatcag ccgggatctc
     7141 accggtctgt gggcaaaccg gaatttgacc ggaggtgaag accatgctgc ctaaatcaac
     7201 gccttgaaca taagggccga ttgcgcctgg ggcacgttgc gtttcgataa tctttttcat
     7261 acatcctccg gcgtcagagc gcctgggtaa aggtacgtga aataacatcc tgctgctgtt
     7321 cacgggtcag tgcgttgaag cgcacggcgt agccagagac acggattgtc aggttaggat
     7381 atttttccgg gtgctcgatg gcatccagca gcatttcccg attcattacg ttgacgttga
     7441 ggtgttgacc gccttcgaca tccgcttcgt ggtggaaata cccatccagc aggccgacaa
     7501 ggttggtttt acgtactgga tcttctttgc ccagcgccgc agggacgatt gagaaggtgt
     7561 acgagatccc atctttggcg taggtgaacg gcagtttcgc caccgacgtc aatgaggcca
     7621 cggcaccttt gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac ggtgttccgg
     7681 cgcgacgacc gtccggcgtg ttaccggttt tctggccgta caccacgtta gaagtgatag
     7741 tcagaatcga ctgggtaggg acggcgttgc gataggttgg cagcgcttta attttcttca
     7801 taaaacgttc aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg ttgccgtact
     7861 gcggatattc accgtcgatt tcaaagtcca ccgccaggcc gtttttgtca cggattggtt
     7921 tcacgcgggc atatttgatc gcagacaggg agtccgttgc caccgacagg cccgcgatgc
     7981 cgcacgccat ggtgcgatag acatcacgat cgtgcagcgc catcagcgaa gcttcgtagc
     8041 tgtacttgtc gtgcatgtag tgaatgatat tcagcgcgct gatgtactgc accgccagcc
     8101 agtccatgaa gtgatcgagg ctgtccatca ctttgtcgta atccagcaca tcgtccatca
     8161 acggtgccgt tttcggcccg acctgaatct tcagcttctc gtccaccccg ccgttaattg
     8221 cgtacagcag tgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt tgcttaccaa
     8281 tcaccattgg gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag tcagtacgca
     8341 tcagatcgtc gttttcatac tgcaaggaag aggtgacgat cgacacctgc gcggcatatt
     8401 ttttgaaggc aatcggtaat tcttccgacc aaagaatggt caggttaggt tccggtgcag
     8461 gccccatagt gtgcagggtg tgcaaatagc ggaaggagtt tttggtcacc agcgtacgac
     8521 cgtccagccc catcccgccg atcacttccg tcgcccagat tgggtcgccg gagaacagcg
     8581 aatcaaattc cggcgtacgc aagaagcgca ccatacggat cttcatgatg aagtgatcga
     8641 tcagttcctg tgcctgctgc tcattgagta cgccagcttt aaagtcgcgc tcaatgtaga
     8701 tatccaggaa cgatgccgtg cggcccagcg acatcgcgcc gccgttctgt gatttcactg
     8761 ccgccagata agcgaagtag agccactgca ccgcttcctg cgcattctgc gccgggcgag
     8821 agatatcaaa gccatatttc gccgccattt cctgaatctg caacagcgca tgacgatgct
     8881 ctgccagctc ctcacgcaga cggatggtgg cttccagatc ctcgcctttt tccagacgag
     8941 actggagatc ggcaaattgc agttcgcgtt cacgcaccag ataactgatg ccatacagcg
     9001 ctacgcggcg atagtcaccg ataatgcgcc cacggccata gccatctggt aaaccggtca
     9061 gcacgccaga tttacggcag cgcagcatat ccggtgagta aacatcaaat acgccctggt
     9121 tatgggtttt acgcagatcg gtaaacagat attcaaattc actgtccatt tcacgaccat
     9181 aggcgtggaa tgaactttta atcatattaa tgccaccgaa cgggtgtaac gcacgtttca
     9241 acggcgcatc cgtttgcagg ccaacaattt tttccagcgg ctggttaata tatcccgcat
     9301 catgagcggt aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg tgggttgcgt
     9361 tttcaatacg aatgccttcc atcacctttt cccacaattc cgtggtggct ggcgtcgctt
     9421 cagcgaggaa agattcatcg ccttcatacg gtgtatagtt atgttgaata aaatcgcgga
     9481 cattaatttc gtttttccag tccgtacctt taaagccaag ccatgcgtcg gcgtacagct
     9541 tatcgctggt atcaatatct accttcatga aaaataatct ctctacaata cttcaactaa
     9601 attatgcaaa ttctgcgggt gcgttaactt tgcctaaatg aatggcatcc aaagcaatca
     9661 ttttttcttc gttggtcgga attacggcgc aaatgacacg cgcattttca ctggaaacaa
     9721 ttcgctcgcc aaaagagtta gatcgattat tcatttctgt atcaatctct acgcctaata
     9781 cagccaaatg ttccatgacc agacgacgaa ttaaacttga attctctcct attccgccgg
     9841 tgaatataat tccatccagg cgacgtaatg aagctgcgtg tccggcaata tgacgggcaa
     9901 ttcggtgaac aaaggtttta attgccagct gcgcgcgttc gtgaccttca tgccaggctt
     9961 tttccagaac acgtaaatcc gaagaaagac cggaaatacc taataatccc gactctttat
    10021 tcactacgcg ttccaggtca cccaggctct ggttggtttg gctggcgacc caggacatcg
    10081 caccaaagtc gacatcgcca ctgcgggtac ccatcatcaa gccttccagc ggcgtcattc
    10141 ccattgaggt gtcaacactc tgaccgttgc gaactgcgca gattgacgcg ccattgccaa
    10201 gatgcgccac aaccaggccg gagtcatctt ccgccagatt cagcaaagaa tgtgcgcgca
    10261 gggaaacata gcggtgcgac gtgccgtgga aaccataacg gcgtacacct aactcttcat
    10321 aatatttcca cggcaggccg tataaataag cttccggagc catcgtctgg tggaaactgg
    10381 tatcaaatac cgccacctga gttacgcccg gaaataattg ctgcgccgat tcaataccac
    10441 ttaaattggc gtaattatgc aggggtgcca gtggagaaac gcgacggata ttatcaatga
    10501 cttcatcggt aataatggcg gactcggtaa aaatactgcc gccgtgagcg atgcggtggc
    10561 caattaaggc cacgctgtca tttaaattcc gtttttccag ttcaaatgca attgccttca
    10621 atgcaccttc gtagctgtgg tgagccagcg gtgctggctc tcccccattt acggataaga
    10681 atgcattttc cgagttaata ccgtcggcaa tacctgacat taatacttca cagtcgctgg
    10741 catcgagtac ggaaaactta atcgaagacg aaccacagtt aataaccaaa acaaccggaa
    10801 attcattcat ctcttttctc atcctgagtt acggattaaa acagtttata tacgatgttc
    10861 aggatggtca gcagaccaat cacggtaaca aacacgttat ccagacgacc acggtatttc
    10921 gccagagacg gcgctttacg gatggcatac atcggcaaca ggcacagcag ggatgcgata
    10981 atcggtgcgc ccatggcttc aatcaggtcg aggatgttcg ggttggcgta ggcaacaacc
    11041 caggtggagc ccatgatgaa gatcatgctg atagtgttca gtttacccag cgatacttta
    11101 gttttgtcgc ctttataacc aaacttcagg accaggccat tcagaccttc cagcgttccc
    11161 agatagtgac cgaagaaaga tttgaagata gccacgagtg cgatgatgga agccgcatat
    11221 tccagtgtaa tcgcgaacgt tgttttggta ccggtcatgg acgcaaagtg gttagccaga
    11281 taagaaagca ctggaatatt ctgcgctttg gcttccgcca tgttggccgg agacagcgta
    11341 aacaggcagc taaaggcaaa gaacatcacc actgcaacca tcagcatgct ggcacgagaa
    11401 atgatttggg aacatttacg ttcggtgaag tcgcgaccga agtctttctc atactcttca
    11461 cgtttagaaa ccacgaagga agagacgatt ggcgagaagt taaaggagaa aaccatgatg
    11521 gaaatcccca gccagacagt gatcaggata ccgtcatgac cggttaacga cagcgaaccg
    11581 aggtcaacct ggtcgataac tgcagagttc cagtaaggga tcagcgacaa agaaatcagc
    11641 accaggctgg cgataaacgg ccataccagg tagctcatca ctttaaccat cagatcctta
    11701 ccaaaccaga tgacgaaagc catcagcagc aacaggaaca gcgccacaaa gccgcgattc
    11761 agcggtgcaa agccgagctg gttttcccag aacgtcataa aggtattggt aatagtaacg
    11821 ccataaatcc acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat aaccacgccg
    11881 ccagttttac caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg gttagagcca
    11941 gaaagacaca gacgcgccag cgcccggtgg caataaaatg cgatggggta tgccaatacc
    12001 aacatcagaa gaatcgggat cagtccgcca aaacctgcgc ggatagggaa gaacagcacc
    12061 ccggcgccga ttgccgtacc aaacaagcct aacgtccatg tggtatctga tttacgccag
    12121 gacgattgtt ttgtctggct ggatacaatg ctatctgaag tactcatatc ctatcctcaa
    12181 cgaattaatt aagcgtcaac gaaaccggtg atttgagaga cgcgagaaag atcgatattg
    12241 ccgccggaaa taatactgac ggtttttctg ttttgaatat attggtctaa tttaccgctt
    12301 aataatgcag cacatgccag agcgcctgcg ccttcggtga cgactttatt gcgctgaatt
    12361 aaggcaatca tactgtttct gatttcgtct tcgctgacca gcacgatgtc atcgactaat
    12421 tcacgaacga tttcgtaagt taaattaccc gggcgggaga catcacaacc atccgccagg
    12481 gtgccggtag ttcggtgcgt ggttatttct ccggagtgga aagaagccgc catgccgtga
    12541 acgttttcag actgtacgcc aataacacga atggtcgggt taatagattt aattgccacc
    12601 gcaataccag caattaaacc gccaccacca attggcacaa tcacgttatc gacatcatag
    12661 agatcttcca taatttccag gccaatcgtt ccctgaccag caatcacttt cggatcatcg
    12721 taaggtggga taaaaatacg gccttccatt tcgacaattt cgctcacttt agcgatagtg
    12781 tcgttgaagt tatcaccatg cagaacgact tctgcggagt agtcgcacgt tgccgctact
    12841 ttggatttcg gcgcgccttt tggcatcacc actttaccgt cgatacccag catcgcgcag
    12901 gagagcgaaa ccccttgcgc atggttgccc gcagaacagg ccaccacgcc tttgcgtttt
    12961 tccgcatcag tcagtgaact taatttatta aatgcgccac gaattttaaa tgaacccgta
    13021 cgctgcatat tttcaaactt caggaatatt tcacctttgc aacgttcact aaaatagttg
    13081 gagcgaggca tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc ttcaataatg
    13141 tcatcaatag caaccggcag atcgtatgta atatgcatta taaaacctct tcgcctgaat
    13201 taaatatgta ggtaaccgta accgacacct gcaagacaga caggtggatt atttatgttt
    13261 aaataaaaac aaataactaa ccaacttcta ttaattgcct tcgtctacac ccattataag
    13321 atgaatactc tttggctaat tccaccaaaa ccgatgctgc ttttttaata cgataatttt
    13381 tcgaccatac cgcggcatat tgtgccacag gtaatgtttc ttcaaccgga atagtaataa
    13441 attgattaga accaaaagga gacgtcatat cacaaggaat tacagttaag aaatcagcat
    13501 tgagaacaag attataaatt gtcacgactg agtcggtttt aacgatgttt tcaatactga
    13561 tgccatttct ttgtaacgta gtaagcagtt cgctgtagta ccccatatta gtttgtggca
    13621 acacccactg ttcgttcttc aacgactcca gcgtggtggt gccggtgcat gttcgggact
    13681 tactggctac cagcacaaac tcggactcga acagcggctc aacatgtaaa tcctgaagct
    13741 tcatttctgc gcttaacgta ccaatcgcaa aatcaagtcg accgtcgcgg attgccggta
    13801 agaacgaaga cagttgcgct tcatacatag aaacctgcgc tttcgggaac acctctttga
    13861 acttgttgat catccctgac ataaaagtaa aaccaatcaa tgaaggaaaa ccaaatgaga
    13921 cttccaccac cgcctcagaa gacataccgc ttatctcatt aaccatattt ttcatttcac
    13981 gggtaatgga ttcggaacga gagagtaaca attgaccggc aggtgttaat gttactccgg
    14041 tatttttccg caccactaat tccacaccaa aataatcttc aatatcgtta atgattttac
    14101 tgacggccgg ttgagttaac cctaattctt ttgcagccga gccgatagaa ccacttctaa
    14161 tgacttcctg aaagactacc aggtgctgcg ttttcggaag aagaatagtg ctcataacga
    14221 cctacgttaa ttacctcatt gacggcatga agtgtatcaa aatgaaatga acaggatatg
    14281 tgcgaccact cacaaattaa ctttcaatac tttccagggt atcgtcatgt caattaaata
    14341 cctttaaata tcaataaatt aaagtataaa aatcagcata aaccctgatt tttgtcaaaa
    14401 aaaatgacgg ggataaccat attttatggc gataacatca ttcgttatgt ggttaataca
    14461 aaaaaggggc tgagaccata tttcaagcaa ttaccggata attaccaggc aaaatttgag
    14521 ttaaatttaa tgagcaagtt ctcaaatttt attataaata aaccattttc agcaataaat
    14581 aatgcggcac gtcacatttt ttcacgctat ttgttggaga acaaacattt attttatcaa
    14641 tattttaaaa tttcgaatac aggtattgat catctcgaac aattgattaa cgtcaacttt
    14701 ttctcttctg acaggacgtc attttgtgaa tgcaatcgtt ttccataaat tcttcttcct
    14761 tcataggcga cgaatagcat tttgtgttga ggatcacaaa acgaataatt gctgatcgcc
    14821 gcgataaggt cagacaaaga caacaaggga aattttcaca gagcttttga tcggcgtagg
    14881 ccacagaatg ttgcttcgtt tactgtaacg ccgggtaaat gagcgttttt tgatagtgcg
    14941 aaagaaccct gcgaggaaaa taagcatcta ttattgtttg tcatgatgac aaggcaggca
    15001 tcaatgaaag gcttcccaat tgcacatatt tttcaccctt caatcccacc aatgcac
//
LOCUS       DABSYT020000107        14727 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.107, whole genome shotgun sequence.
ACCESSION   DABSYT020000107 DABSYT020000000
VERSION     DABSYT020000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.107"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            203..1033
                     /locus_tag="ID725_RS19590"
                     /old_locus_tag="pgaptmp_003918"
     CDS             203..1033
                     /locus_tag="ID725_RS19590"
                     /old_locus_tag="pgaptmp_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGV7852409.1"
                     /translation="MSMPLSNVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1174..1947)
                     /gene="uxuR"
                     /locus_tag="ID725_RS19595"
                     /old_locus_tag="pgaptmp_003919"
     CDS             complement(1174..1947)
                     /gene="uxuR"
                     /locus_tag="ID725_RS19595"
                     /old_locus_tag="pgaptmp_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGV7852410.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2162..3622)
                     /locus_tag="ID725_RS19600"
                     /old_locus_tag="pgaptmp_003920"
     CDS             complement(2162..3622)
                     /locus_tag="ID725_RS19600"
                     /old_locus_tag="pgaptmp_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGV7852411.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3703..4887)
                     /gene="uxuA"
                     /locus_tag="ID725_RS19605"
                     /old_locus_tag="pgaptmp_003921"
     CDS             complement(3703..4887)
                     /gene="uxuA"
                     /locus_tag="ID725_RS19605"
                     /old_locus_tag="pgaptmp_003921"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGV7852412.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            5227..6570
                     /gene="gntP"
                     /locus_tag="ID725_RS19610"
                     /old_locus_tag="pgaptmp_003922"
     CDS             5227..6570
                     /gene="gntP"
                     /locus_tag="ID725_RS19610"
                     /old_locus_tag="pgaptmp_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGV7852413.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6744..7646)
                     /gene="fimH"
                     /locus_tag="ID725_RS19615"
                     /old_locus_tag="pgaptmp_003923"
     CDS             complement(6744..7646)
                     /gene="fimH"
                     /locus_tag="ID725_RS19615"
                     /old_locus_tag="pgaptmp_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGV7852414.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7666..8169)
                     /gene="fimG"
                     /locus_tag="ID725_RS19620"
                     /old_locus_tag="pgaptmp_003924"
     CDS             complement(7666..8169)
                     /gene="fimG"
                     /locus_tag="ID725_RS19620"
                     /old_locus_tag="pgaptmp_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HGV7852415.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8182..8712)
                     /gene="fimF"
                     /locus_tag="ID725_RS19625"
                     /old_locus_tag="pgaptmp_003925"
     CDS             complement(8182..8712)
                     /gene="fimF"
                     /locus_tag="ID725_RS19625"
                     /old_locus_tag="pgaptmp_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGV7852416.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8722..11358)
                     /gene="fimD"
                     /locus_tag="ID725_RS19630"
                     /old_locus_tag="pgaptmp_003926"
     CDS             complement(8722..11358)
                     /gene="fimD"
                     /locus_tag="ID725_RS19630"
                     /old_locus_tag="pgaptmp_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGV7852417.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11425..12150)
                     /gene="fimC"
                     /locus_tag="ID725_RS19635"
                     /old_locus_tag="pgaptmp_003927"
     CDS             complement(11425..12150)
                     /gene="fimC"
                     /locus_tag="ID725_RS19635"
                     /old_locus_tag="pgaptmp_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGV7852418.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12187..12726)
                     /locus_tag="ID725_RS19640"
                     /old_locus_tag="pgaptmp_003928"
     CDS             complement(12187..12726)
                     /locus_tag="ID725_RS19640"
                     /old_locus_tag="pgaptmp_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7852419.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12791..13339)
                     /gene="fimA"
                     /locus_tag="ID725_RS19645"
                     /old_locus_tag="pgaptmp_003929"
     CDS             complement(12791..13339)
                     /gene="fimA"
                     /locus_tag="ID725_RS19645"
                     /old_locus_tag="pgaptmp_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGV7852420.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(13804..14400)
                     /gene="fimE"
                     /locus_tag="ID725_RS19650"
                     /old_locus_tag="pgaptmp_003930"
     CDS             complement(13804..14400)
                     /gene="fimE"
                     /locus_tag="ID725_RS19650"
                     /old_locus_tag="pgaptmp_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGV7852421.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT         3678 a         3705 c         3479 g         3865 t
ORIGIN      
        1 tatgatttta aattgcatgc aaataatttt aaatgagcgt tttttgatac tttagagtgt
       61 ttcctaccct ttaatatcac tgtgtagagt tgatatcact acaacagaaa catgcatgtc
      121 atttgtttac ttctcttttt actacagaaa aatttatcta tttttctaaa catcagtgaa
      181 atactcataa aaggtaacta ttatgtctat gccattaagc aatgtccttc agagtcaaat
      241 tattacagat aatcacttcc ttcatcatcc aaaagtcgaa agtgagttaa cacgtaaata
      301 tgaacgtgca cgcctggata cggaaaatat ctatcttctg cctcttgctc gtggcaataa
      361 ccataattac gatggaaaaa gcgttgtcga aattagaaaa cttgatatta gtaaagaatc
      421 ctggccattt aattatgtga cagaagcatg cagagaatcc gatggtatta caacaacagg
      481 acgtatgctc tatcgaaact tgaaaatcac ctcagcttta gacgaaatat atggtggtat
      541 atgtaaaaag gcgcatgctg ctacagagtt agcagaaggt ttacggctaa atttatttat
      601 gaagagccct ttcgatcctg ttgaggacta tacagttcat gaaataacat taggccctgg
      661 ttgcaatgtt ccaggttacg ccggaaccac gataggatat atttcaaccc ttccagcttc
      721 tcaggcaaaa agatggacca atgagcagcc aagaattgat atctatattg atcagataat
      781 aactgtctca ggggtagcaa attcttccgg attcgccctt gccgcattac ttaatgctaa
      841 tattgagttg ggtaatgacc caataattgg tatagaagcc tatccaggta ccgcggagat
      901 ccatgcaaaa atgggatata aagttatccc cggtgatgaa gatgcgccac tcaaacgcat
      961 gactctgcaa ccgtcatctc ttccagaact gtttgaatta aaaaatggtg aatggaatta
     1021 tattggcaaa taaccactga gaagaatgag tactaacatt caggttatat agatatttaa
     1081 acaataccct gcaatttaca aagtaatact cattgcgact tcttaattta agaaagcgca
     1141 aggaacgttt acccttgcgc ttattataat aagtcaggcg tcgactttat ccagcggcca
     1201 tgaatcaaac agatagccgt caaaataaat atcgtcaacg ttcgagaatt ccagcagacg
     1261 ttgcttaacg ttttccagat gctgccacat tgccagcttc gccgctcggg catctttttt
     1321 gattaacgcg gcgaggatct gtttgtgatc gcccaaccac tctttgcgat acaggctgtc
     1381 atccagatgg ctgtgcaact ggatccacat tgggttgttt tcccgccact gccaggactg
     1441 acggaacagc tccaccagca tgctgttatg cgttgcttcg gcaatagcga gatggaactg
     1501 catgtcaccg ctttcgctgc tgcccggcgc actggaagcc agttcacgct cttccagttg
     1561 caatgcctga cgcattttga cgatatcttc gcgggtagcc tgcagggcgg caaactcggc
     1621 gatgttactt tccagtaact gacgcgcctg taacagctca aaaggacccg catcattgca
     1681 gacgttggca tccggactgt ctgtgttgtg gctgcctgag ctatcaagaa catagatacc
     1741 ggcaccccgg cgcacttcca ccagcccttt gatctccagc atgatcagcg cttcacgtac
     1801 caccgtccgc gtgacatcaa gcatttctgc aatttcacgc tccggcggta gccgttcacc
     1861 aggattgtac ggcgtcttta tgatcagatc gcggatcatc gccccgactt cctggtaagg
     1921 tctttgcgca gaggtggcag atttcatggt gttgtgtccg ttaggtgcgg ttaatcaaat
     1981 ttttgccgga tgcggcgtga acgccttatc cggcctacaa cgggcaattt tacggccccc
     2041 gttaatctca aactatacca ttagcactgg aagttggcac gtacaggtgg gcaaaaaaac
     2101 gaaaactggt caaccaaata tcagtttgat tgaccagtaa gtctgtaggg taatcagtta
     2161 gttacagcgc agccacacac tcgcgcgcac cgcgttcgca cagctgctga tatgccgcag
     2221 tcaccgcgcc aacaaagtcg gcattctgcg gcagatcatc ggcaaaaata ccgctcaggc
     2281 ccagcagcgc tttcacgcgg tctgcgccct gatactgcgc gttaatcttc tggaactcgg
     2341 ccagcatcgg gtcgaccacg tcaatggcat taccctgctc atccacgccc tgggtgtaac
     2401 gcatccagcc agccacgccc agcgccaggt gacgccagct gccgccgttt tgcaggtgca
     2461 gacgcaccgg gtccagcaga cgctgcggta acttctggct gccgtccatc gcaatctgcc
     2521 aggtacggtg acgcagagac gggttgctga aacgctcgat cagcagcgtc gcataggcgt
     2581 tcaggtccgt accttccggc atcgacagcg ttggcgcttg ttcctgcatc atcagggcaa
     2641 aggccgcttt gcgataatcc gggttagtca tggtgtcggc aatagtttca tagccgccga
     2701 ggtaaccgag gtacgccagg aaagagtggc tgccgttcag catacgcagt ttcatcattt
     2761 cgaacggcac aacgtctgca acgaactgtg cgcccacttt atcccagtcc ggacgaccat
     2821 taacgaagtt atcttcaata acccactgac ggaacggttc gcaggcaatg gcgcacgggt
     2881 cgtaaacacc cagctggtca gcaatttcct gtaaggtttc tggcgtcgcc gccggaacga
     2941 tgcggtcaac catggtgcac gggaaggtga cgttctcttc aatccatgcc gctagctgcg
     3001 ggtcacgcgc ctgagccagc cccagtaccg cgaccttcgc cacatgaccg ttttcacgca
     3061 cgttatcgca ggacatcacc gtaaatgctt tcagcccttt ttcacgacgc agacgcaagg
     3121 cttcgacgat gtaaccaatc gcggacttcg gcgcagtcgg gttttccaga tcgtgcttga
     3181 tcagcgggtt attgagatcc agttgaccgc ttgccgcatc agcgcagtag cctttttccg
     3241 tgaccgtcag ggagacaatc gccgtttgcg gacgcgccat cgcgttgaga ataccttcgc
     3301 agccgtcgat ttccgggtgc agcgcttctt tcatcgaacc gataattttc agctcggtgc
     3361 tttccgcgcc tttttccgcc acggtgtaca gcagttgctg ttttttcagg ttttcgatca
     3421 gcacgcggtc gttgcctggc atcaggttaa cttcgcagat accccagtcg ctgtcggtgc
     3481 tttccagcag atgatgggtg tacagcgcct ggtgcgcacg gtgaaacgcc ccgcaaccga
     3541 gatgcacaat gcgtgattcc agacgagaat gatcccatga cgggcgggca accggcagat
     3601 tgctgtcaac aatagtagtc atcgtaaact ccagaatccg tcttcagaga gtgacggcaa
     3661 ggaagaagga gccggcacgg cgcgcagcca tgccggtggg tattaatggc taaagaaagc
     3721 gcgctggatc gccagttcga caccgcgaac ttcggccagg cctttcagac gaccaattgc
     3781 ggagtaacca gggttggttt tcttcttcag gtcgtccagc atctgatgac cgtggtccgg
     3841 acgcatcggg atcaggtctt ctttgccttc cgctttacga cggtgttctt cttcaacaat
     3901 cgctttcacc acttcgtaca tatcaacgtc accgttcagg tgcgccgctt cgtggaaggt
     3961 tttcgggtta tcttcacgca tggtggagcg cagatgggtg aagtaaatac gcggaccgaa
     4021 ctgcttgatc atatcaacca aatcgttgtc agcacgcacg ccgtaggaac cggtgcacat
     4081 ggtgaaaccg ttcgccatgc tgtttacggt atcaaccatc cactgcatat cttcaatggt
     4141 ggaaacaatg cgcggcaggc cgaggatcgg gcgcggcgga tcgtccgggt gaacagccat
     4201 acggacgcca acttcttcgg caaccggaat aatcgctttc aggaagacag caaagttttc
     4261 gcgcagtttg gctttgtcga tatctttgta cagctccagg tgcttacgga actggtcgag
     4321 ggtgtaccct tcttccgcac ctggcagacc agcgatgatg ttacgggtca gacgcgcttt
     4381 gtcttcatcg ctcatagtgg cgaagcgttc agctgcctga gcaatttctt cttcggtgta
     4441 atccgcttcc gcgcctggac gtttcaggat atgcatttcg aatgcagcga attcgatctg
     4501 gtcgaagcgc agagctttgg agccgtctgg cagcacgtat tcgaggtcag tacgggtcca
     4561 gtcgagcacc ggcatgaagt tgtagcacac ggtgcgaatg ccgcactgcg ccaggttgcg
     4621 cagagtctgc tgatagttag cgatccactg ctcatagttg ccagtgtggg ttttgatatc
     4681 ttcgtgaatt ggtacgcttt ctacgacaga ccacaccagg cctgcgtctt caacgatcgc
     4741 cttgcgtttg aggatctctt ctacggacca tacttcgccg ttcgggatat ggtgcagcgc
     4801 ggtaaccacg ccagttgcgc ccgcctgacg gacatcagct aaagaaaccg gatcgtttgg
     4861 gccgtaccag cgccaggtct gttccatgat ttttcctctt tcacatagtg ggttccacag
     4921 caaagagacg cggaaccctg gagttgttta ggtatccgga tgaacaacca gaccgcagcc
     4981 gtggcgttgc atcggatgag atggcgtata agttctacct tttggtaaga catgtcaacc
     5041 cgaattctga aattggttaa ccacatcaca agaatttcac tctcatggtt gttgctgcaa
     5101 attgcagcaa agggtaatcg atggttattg atcagaggat gtgacattca tcgcaacaat
     5161 ggttgaccaa tttacataac atgtcgccca aaataacaac ggttcaacca ggtaaggtaa
     5221 aacgttatgc atgtgcttaa cattctctgg gtggtattcg gcattggtct gatgctggta
     5281 ctgaatttga agttcaaaat caattcaatg gtggctttgt tggtggcggc gctgtccgtc
     5341 gggatgctgg cgggcatgga tttgatgtcg ctgctgcaca ccatgaaagc gggcttcggc
     5401 aacacgctgg gggaactggc tatcatcgtg gtgttcggtg cggtcatcgg taaattgatg
     5461 gtcgactccg gcgcggctca ccagatagcg catacgctgc tggcgcgtct cggtctgcgc
     5521 tatgtacagc tgtcggtgat tatcatcggc ctgatttttg gtctggcgat gttctatgaa
     5581 gtggccttta tcatgttagc gccgctggtt attgtcattg ccgccgaagc taaaattccg
     5641 ttcctgaaac tggcgatccc ggcagtagca gctgccacta ccgcacattc actgttccca
     5701 ccgcagccgg gtccggtggc gctggtgaat gcttatggcg cagatatggg gatggtttat
     5761 atctatggcg tactggtgac gatcccaagt gtaatctgcg caggtctgat cctgccgaag
     5821 ttcctcggca atcttgagcg cccaacgcca tcattcctga aagcagatca accggtagat
     5881 atgaacaatc tgccctcttt cggcgtttcg attctggtgc cgctgatccc ggcgatcatt
     5941 atgatctcta ccaccatcgc caatatctgg ctggtaaaag atacccctgc ctgggaagtg
     6001 gttaacttta tcggttcctc gccgattgcg atgtttattg cgatggtggt tgcattcgta
     6061 ctctttggca ccgcgcgtgg tcatgacatg cagtgggtga tgaacgcttt tgaaagcgcg
     6121 gtgaagagta ttgcaatggt gattctgatc atcggtgcgg gtggcgtgct gaagcaaact
     6181 atcatcgaca ccggcattgg cgacaccatc ggcatgttga tgtcccacgg caatatctcg
     6241 ccctacatta tggcatggct gatcactgtg ctaattcgtc tggcgacggg tcagggtgtc
     6301 gtttcggcga tgaccgccgc cgggattatc agtgctgcaa tccttgatcc agcaactggt
     6361 cagctggttg gcgtgaatcc ggcgctgctg gtgctggcga cggctgcggg ttccaacacc
     6421 ctcacccaca ttaacgatgc atctttctgg ttgttcaaag gttactttga cctgtcggta
     6481 aaagacacgt tgaaaacctg gggtctgctg gagctggtca actccgtggt tgggctgatt
     6541 attgtgctga ttattagcat ggtagcgtaa aagaacacca gagcctgcca atggcaggct
     6601 cagttcggat tgtcggcaaa gtgcgctttg tttatgccag atgcgacgct gacgcgtctt
     6661 atctggccta caaagggcta acgtgcaggt ttttagcttc aggtaatatt gcgtacctgc
     6721 attagcaatg ccctgtgatt tctttattga taaacaaaag tcacgccaat aatcgattgc
     6781 acattccctg cagtcacctg cccgccggta cgtgcgtaat ttgccgttaa tcccagactt
     6841 accgccgaag ttcctactgc tcctaacgat accgtgttat tcgctggaat aatcgtaccg
     6901 ttgcgcgtca actgtacgcc gacgccctgc gctggtgaaa acgacgcggt attggtgaaa
     6961 atcgagttgc ccgcatctgc ggttgtgccg gagaggtaat accccaggtt ttggcttttc
     7021 gcacaataaa cggtaagagg aattggcact gaaccagggt agtccggcag agtaacggtg
     7081 acatcacgag cagaaacatc gcagccgcca gtaggcacta ccacatcatt attggcgtaa
     7141 atattccaca caaactggaa atcatcgctg ttatagttgt tggtctgtcg caaaataagc
     7201 acggcaatta atgagccagc tttaatcgcc accccgcccg cactgctcac aggcgtcaaa
     7261 taaagcgcca ccggccacgg cttatccgtt ctcgaattat aaacaacccg cggcgtttcg
     7321 ctggtggtcg gaaatggata gctactgcca ctatatttta cggtcccgga aaaattagat
     7381 aacacgccgc cataagccga gcctcgttgc agtgtgacat agtctgtaat ggtttccgga
     7441 taatcgttat ggcaaaagat ttgcgtcgaa agatctacga ccaggttttg ccccacattc
     7501 acggcaggcg caaggtttac ataaacatta gcgctgccac cgccaatagg gatagcggta
     7561 ccattggcgg ttttacaggc gaatgaccag gcatttaccg accagcccat cagcagtaca
     7621 gcaaacaggg taataactcg tttcattaca attatctctt cgggttcagc tgtaggtata
     7681 ggtgatgcta atcactgcct gaatggttcc ctgagtggct ccgccattta ccgtcaatgc
     7741 tctgacctgt aacgggaagt gcgctgattg tgaggaatca tccacctgaa ctgttttggt
     7801 tgcgccagta ttcaatgtgt tgccactgtc atcctgtagc tctaactgga tgttttgcgc
     7861 ggtcccctgg tttttataat atccggtact gtcggctgcc ccgctgaagc tggcagtgac
     7921 ccttgacgtt cccaccggac aattagtcaa ctcaagcgca acatcatgcc aggccgatgc
     7981 cgccccggca gacatcagac tgaaagaata aagatcgccg agatcaaccg tggcattggt
     8041 ggtggaaact gtgcacggtt tggcgacgac cttaccgttc accgtgatgg tgacatcggc
     8101 tgcctgtatc gtcgcacttg cgagcgccaa tattgccgcc aataaatacc cacgtttgcg
     8161 ccatttcatg agcatctcca gttactgata ttcaagagtg aaggtagccg tggcattgat
     8221 atgccccgca gtgacaggca cctgtgtcgc cattagccgg gcgtaaaaat tcaacgtatt
     8281 tggtttaccc ggcgtcaggg tcgtccacga aatcgcggac gatggagcat taaggggtat
     8341 ctgattttgc tgctcattca gaagctgtat tcccagtccc gaagccgctg acaccgtatt
     8401 ttcaagtgca agcaggttgg cattgtggct atctgcaacg ccggtaaacc caacttttac
     8461 ggcagaaacg gcgttaccac agggtgacag caaaatacga aatggaacga caggagtcgt
     8521 cgcgccaatg ttgttaaatt gcttcgccgc gttttccatc agatcaacag taaaattggt
     8581 tgattcagcg gccacactac agccgttatc tctgacatag ccgcggatag taatcgtgct
     8641 atccgcagcc aaagcgtgac tcactgccag ccacaaaaaa gcgcacagaa gataaaaagg
     8701 tttgtttctc atcacgcccc cttaacgaca ttcagctgat agctgggtta ataactgctg
     8761 ctgactctct ggtggcagtt gataattggc gacacaatga gcattttcct cttctcccca
     8821 tttcacctga acttttcccg ctagaggcat tccgctgagg taaacctgac cattatccgc
     8881 aacaatgccg ctactctggc tactctctga tgtcaccatc gccccaaacg gcagcggctt
     8941 attattgtgg gttagcgtca tgagcagttt tatcccaacg cgcgctttaa actctgctcg
     9001 cacgatcgcc ccacgagtgg gaacaacgtt agcgaccgcg ttatctaaat cgacgttatc
     9061 agccagggta ttggtatcca gcgccactct attttcccga tattcagtgg cataaggcag
     9121 cacggcataa ccgcgccagt cggtacgcac ccccgtctgg ttttcgactt ttgcatcttt
     9181 tgcgccaggc gctttaacaa gcaccaccgt atcgtttaac ggctgcccca gcgttacgcc
     9241 attggcatga gccagtaccc caccgctgac tccgtaatag agctgcttaa tatcatcgct
     9301 atggctgtaa ccgatattgg cattgccgta accaccgcga taattcagcg tggcgtagcc
     9361 tgtgcttccg ctattaccat cgcctccccc ggcatagccg gtttgcacgc tatagctgag
     9421 gttgttgtct tccagcaacg taccgtatac accagccaga ttggtcatcc gaccgttgag
     9481 atcgtgtgac atgctgtagc tggcactggc atgtcgccac tgagatttac tgtcagaacg
     9541 cagccagtgg ctgaaaggaa tattgacgtt acgcgctaac atctgatcac gtcctttttg
     9601 ccaggcgttt ttcgtcaggc tatagctgag cgtccagttg atatcttcga acgcagtatt
     9661 taatccagcc tggaattgct catcgacatt actcgttccc caataagttt gatggctacc
     9721 actcaaatac agtgttgatg agcgcccgag ttgctgagta acggtgagtt gtaatttccc
     9781 gcgtttgtta taagcgaggt tgtaatagtc ggtgaatttc ggcttaacct gaataactcc
     9841 gtcctgtgtt tcgatgttgt agccattcat tcgactgtat gttgtatcag cgaaattaaa
     9901 atatccgctg gtcgaataac ggtaacccac taactgaata ttcgtgcctg actcattgag
     9961 cgatttgtta tagagaaaac gcaccgattg tccgtcatgc tgactgtcat cgggaagtgt
    10021 ggaattagcc tgagtcatat ccacagacag agcgcccagt gcccccatat ttttcccgat
    10081 accaaaatta aaagcacgat aacgatctgc cagttgcgtt ccaccatata ttgtccagcc
    10141 agctggaagg ccgtggagta atgtactttg gaaaaagcgg ggtttttcct gttgcgcatt
    10201 tccactacgg tattctcctg ccgtaatgga ataacgagta tgcccttcac gttgcaaaag
    10261 cgggactgac gaatagggta cggtaaaaat ctgcgtgctg ccgtcagcct ctttaatcgt
    10321 tacctgcaag tcaccactat tacctgcggc atagatatcg ttgatggtaa aaggccccgg
    10381 cggcaccgta ctattataaa tgtcataccc attttgttta atagtgacct gtgcagtacc
    10441 acgagcaata ccgtggatca ccggggcaaa tcctctttgg ctatcgggta acatattgtc
    10501 atctgaggcc aattgtgcgc cgcgaaagtt aataccatcg aaaatatcac cctgagtata
    10561 accatcaccc agcgtcagcc gggaacgtaa cggtattatg tctcgctcaa gccaggtatt
    10621 gatatgctgc catttatttt tgctacctga tgatctgtcg ctactgttat aactccaggt
    10681 ggtattgtcg cgtaaacgcc acgcaccaat atttaaccca ctctgtaggt ttaaatatgc
    10741 ataatggctg ttacccccaa tccgattctg tacactattt ccgctgaaat tataattgag
    10801 caatccggca ttaataccgg gatcccataa ctcaggagga atataaccac gcgcgcgatt
    10861 actcataaat gcctgaggga tcgtcaggtt cagtcgctgc tgaccaacat ctaaatgcgc
    10921 agtagcgtcc tggaccattg tggttaatgg cacacaggca tcatccgcca gcagattcat
    10981 accggcgaca gaagccgtat tcagccccat actggcgagt tgcgcgcgtg tcaggcaggg
    11041 aacaatccct tgttcactgt cgcccgtatt aaatgtgaca tcacgcgttg ccatataacc
    11101 attattcaaa tagatatcga cgcgatacgt ccctggcggt aattcttgcc cattttcaaa
    11161 acgcgataaa tcggccacag cctggggatc atccgctaaa aagcgcggat taaaatagag
    11221 ttcggcagat gacaaaggtg cctgtacggc aaaagcacag gcgacaaaga gccggacaaa
    11281 aaaaccagcc aaacgatgct tacgaatatg caagcattgt gtgtttcgct ggtaaagtct
    11341 taaattcaga tatgacatta ttcctccggt ttaaaatcac ccggcagtca attcttttta
    11401 ttcaggcgaa aaattccccc tgcgttattc cattacgccc gtcattttgg gggtaagtgc
    11461 accataatca tttattgttc ggtaagtaat attgcttccc gcatcagaag gcaatttaac
    11521 cgtgctttcg cccattggag gcaccaaggc attttcaaga acccgggctc cggcattcaa
    11581 ctctgttacc gtcaggtaat agggtgtcgg gttaatcagc gtcagagaat tcgcgctacg
    11641 acgaaatctt aatttttctg cggcctgatc gggtggcaac gctaatttag ccggacgata
    11701 gtacagttta atgcggctga taattgcgag ctgtagcgta ttctcagtca attttgattt
    11761 atccattgac ggaatcgctt taacgttcat ccagaataaa ctttcccggt cctgtggcaa
    11821 ttggttattt gttgcatcaa gaatacgtaa ggtattctct ttttttccct tcatcgcaaa
    11881 cagaggaggc gtcacgataa aacgaccatc ctttacacca tcggcatttt ccacccatga
    11941 ttgaattaaa taggtactat tttcatcatt atttgtcacg gcaagttgca cttgtttttg
    12001 ccctgccgga taaattatgc gagtcgcacc taaggccact cccgcttcag cgcgtccggc
    12061 aaccatcatt gccatgaaca tcaggatacc tgccagcaag cagaatgtta tttcctgcga
    12121 tttccttaca ttgacgtttt tattactcac tgtcctgttc ctgttatcac attatctgct
    12181 gaacaattac tgataggtta aagagaacca ggcctgtgca ttggcgatgc cgccagtaac
    12241 ccgacgcccg gtagcacgat atttggcgat gaaatgtagc gaagtagagc ctgagtaaag
    12301 ccgtttccag tttgctggag gacgattaat cggtacgagg tttccttcat catcaaacaa
    12361 cgctacgcca atattggtgg ctatccctgg cccctctccc acggaaagca catccggatt
    12421 tttaccatcc gcgacaccgt gaaacgccac acccacacgt tcactcacca ccgtgctaca
    12481 ttcccgtaaa tgaataacaa aaggcaccgg tgcgctatct tcccctaccg catgaaaccg
    12541 gttactgctg atttgcccca tattgaccgt catttgttta tcaccggctt caatcaggca
    12601 agtttccgca ataatgacgc cctgaaattg catatttccg ccgggcggcg tggtattcca
    12661 tttatttccg gccagagcaa acattggcag caacgacgct aatagaaata atctcttcct
    12721 tttcatcgtt atttttatcg cacaagggtg ggcatccctg cccgtaatga cgtccctgaa
    12781 cctgggtagg ttattgatac tgaaccttga aggtcgcatc cgcattagca gcacccgggg
    12841 ttgcctcgcc gattgcataa taacgcgcct ggaacggaat ggtgttagta ccgttattca
    12901 gggttgtttg ctcactgaat gtcgcaccat ccagcgtcag cgcagcaccc gttctgtcca
    12961 ggatctgcac accaacgttt gttgcgctac ccgcagctga actctgcaga gccagaacgt
    13021 tggtatgacc cgcatcaatc gccgtaccta aaaaggcaac agcggcttta gatgcaacat
    13081 tggtatcgca atcattcagc tgaatgttaa aaccgacagc agaactggtt gctccgtcct
    13141 gtgccagcga tgcggtacga acctgtccta actgaacggt ttgatcaaca gagcctgcat
    13201 caactgcgca agcggcgtta acaacttccc ctttaaagtg aacggtccca ccattaaccg
    13261 tcgtggcagc ggccagagcc gctgtagaac tgagggacag agccgacaga acaacgattg
    13321 ccagagtttt aattttcatg ctgctttcct ttcaaaaaac tatttctaaa tcgacatggg
    13381 cagtcgttct gtacactttg ttttgtcaac gagtttgact gccaacactg cacagttttc
    13441 ccccaaaaga tgaaacattt ggggccattt tgactcatag aggaaagcat cgcgcacaaa
    13501 ctttttcagt ttatttgctg gattaatatt ctattgttat ctttatttat agatttttat
    13561 attgcatgag gtggtttttg gagagaagaa tgaggaagat gcgtcgagcc acagaaacgt
    13621 tagctttaca tatagcggag gtgatgtgaa attaatttac aagagaaata atttacatat
    13681 caaacagtta gtttaaaatt tttatgcttg agaaaaaata cgtaacttat ttatgatatg
    13741 gacagtttgg ccccaattgt cttgtattta tttgtttttt aactttatta tcaataagtt
    13801 aaatcaaact tcttctcttt ttaatttttc gtttatgaga ttatttcttt cccataatcc
    13861 ggcaaaacga gcagcattac tggcggtata acgtacagta tggcgaatat ttcgatgccc
    13921 gagataatcc tgaattaaac gagtatctgc accacgctcc gccagttcat aaccgcaagc
    13981 atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac cggcatcacg
    14041 aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa taaatatggc
    14101 gtcggtccgg tcagcgcctt tccagttagc acgttctagg gtccagcgtt ccacagcttc
    14161 acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt
    14221 tattcggcct tcattaaggt caagatcctg ataatgcaga tcaagcagtt cactaatacg
    14281 catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg ttgccccgta
    14341 acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac gtttactcac
    14401 aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa cctcaatcgg
    14461 tcaaaattag cccaatagat aaaaagataa ccacacacga agtgatgtgg ctatcagtca
    14521 attacattaa atatcataca aaaataaaat atcactgatg tgataacatc tattttcatt
    14581 gatttgattc aatataacaa tagattgcag tggattaact tccagttgct taaaaaataa
    14641 aggaaaaagt gcagaaacag gagagtttaa gtgatgtgta aatttatgaa tgattttgtt
    14701 tatttatttt gaagaacata aaccatt
//
LOCUS       DABSYT020000108        14360 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.108, whole genome shotgun sequence.
ACCESSION   DABSYT020000108 DABSYT020000000
VERSION     DABSYT020000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.108"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(74..826)
                     /gene="tcyN"
                     /locus_tag="ID725_RS19655"
                     /old_locus_tag="pgaptmp_003931"
     CDS             complement(74..826)
                     /gene="tcyN"
                     /locus_tag="ID725_RS19655"
                     /old_locus_tag="pgaptmp_003931"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGV7852422.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(823..1491)
                     /gene="tcyL"
                     /locus_tag="ID725_RS19660"
                     /old_locus_tag="pgaptmp_003932"
     CDS             complement(823..1491)
                     /gene="tcyL"
                     /locus_tag="ID725_RS19660"
                     /old_locus_tag="pgaptmp_003932"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGV7852423.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1506..2492)
                     /gene="dcyD"
                     /locus_tag="ID725_RS19665"
                     /old_locus_tag="pgaptmp_003933"
     CDS             complement(1506..2492)
                     /gene="dcyD"
                     /locus_tag="ID725_RS19665"
                     /old_locus_tag="pgaptmp_003933"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGV7852424.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2597..3397)
                     /gene="tcyJ"
                     /locus_tag="ID725_RS19670"
                     /old_locus_tag="pgaptmp_003934"
     CDS             complement(2597..3397)
                     /gene="tcyJ"
                     /locus_tag="ID725_RS19670"
                     /old_locus_tag="pgaptmp_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGV7852425.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3485..4036)
                     /gene="fliZ"
                     /locus_tag="ID725_RS19675"
                     /old_locus_tag="pgaptmp_003935"
     CDS             complement(3485..4036)
                     /gene="fliZ"
                     /locus_tag="ID725_RS19675"
                     /old_locus_tag="pgaptmp_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGV7852426.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4082..4801)
                     /gene="fliA"
                     /locus_tag="ID725_RS19680"
                     /old_locus_tag="pgaptmp_003936"
     CDS             complement(4082..4801)
                     /gene="fliA"
                     /locus_tag="ID725_RS19680"
                     /old_locus_tag="pgaptmp_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGV7852427.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5121..6878)
                     /locus_tag="ID725_RS19685"
                     /old_locus_tag="pgaptmp_003937"
     CDS             complement(5121..6878)
                     /locus_tag="ID725_RS19685"
                     /old_locus_tag="pgaptmp_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HGV7852428.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            7144..8541
                     /gene="fliD"
                     /locus_tag="ID725_RS19690"
                     /old_locus_tag="pgaptmp_003938"
     CDS             7144..8541
                     /gene="fliD"
                     /locus_tag="ID725_RS19690"
                     /old_locus_tag="pgaptmp_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGV7852429.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            8566..8976
                     /gene="fliS"
                     /locus_tag="ID725_RS19695"
                     /old_locus_tag="pgaptmp_003939"
     CDS             8566..8976
                     /gene="fliS"
                     /locus_tag="ID725_RS19695"
                     /old_locus_tag="pgaptmp_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGV7852430.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            8976..9341
                     /gene="fliT"
                     /locus_tag="ID725_RS19700"
                     /old_locus_tag="pgaptmp_003940"
     CDS             8976..9341
                     /gene="fliT"
                     /locus_tag="ID725_RS19700"
                     /old_locus_tag="pgaptmp_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGV7852431.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9419..10906
                     /gene="amyA"
                     /locus_tag="ID725_RS19705"
                     /old_locus_tag="pgaptmp_003941"
     CDS             9419..10906
                     /gene="amyA"
                     /locus_tag="ID725_RS19705"
                     /old_locus_tag="pgaptmp_003941"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGV7852432.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(10940..11353)
                     /gene="yedD"
                     /locus_tag="ID725_RS19710"
                     /old_locus_tag="pgaptmp_003942"
     CDS             complement(10940..11353)
                     /gene="yedD"
                     /locus_tag="ID725_RS19710"
                     /old_locus_tag="pgaptmp_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGV7852433.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11540..12745
                     /gene="yedE"
                     /locus_tag="ID725_RS19715"
                     /old_locus_tag="pgaptmp_003943"
     CDS             11540..12745
                     /gene="yedE"
                     /locus_tag="ID725_RS19715"
                     /old_locus_tag="pgaptmp_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGV7852434.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12742..12975
                     /gene="yedF"
                     /locus_tag="ID725_RS19720"
                     /old_locus_tag="pgaptmp_003944"
     CDS             12742..12975
                     /gene="yedF"
                     /locus_tag="ID725_RS19720"
                     /old_locus_tag="pgaptmp_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGV7852435.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13084..13752
                     /locus_tag="ID725_RS19725"
                     /old_locus_tag="pgaptmp_003945"
     CDS             13084..13752
                     /locus_tag="ID725_RS19725"
                     /old_locus_tag="pgaptmp_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGV7852436.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            13863..14342
                     /locus_tag="ID725_RS19730"
                     /old_locus_tag="pgaptmp_003946"
     CDS             13863..14342
                     /locus_tag="ID725_RS19730"
                     /old_locus_tag="pgaptmp_003946"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGV7852437.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT         3499 a         3679 c         3588 g         3594 t
ORIGIN      
        1 tttaagcagt tttatcaggc ttgaaatggc gttccgcccc gactgtcgaa tcgtcggggc
       61 tgattttttc ttattattgc agcagaaact tctcgaggaa ctggcgggtg cgaggctgct
      121 cgggatcggc aaataacgct tttgcggccc cctgctcgac tatccgcccc tggtccataa
      181 aaatcgcccg gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca atcaccatcg
      241 tgcgcttttc ctgcgccagc tgacggatgg tgttcaggac ttcgcccacc agctctggat
      301 ccagcgctga cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc agcgcacgcg
      361 caatcgcaac acgctgctgt tgaccgccag acaaacgacg tggatagctg gtttctttac
      421 cggccagccc aactttcgcc aacagcttgc gagcgcgcgc cgtggcctct tctttcggtt
      481 cgcctttgac gatcaccggc ccttcaataa tgttctccag caccgtacga tgcggaaaca
      541 aattaaagtt ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc agcgattttt
      601 gctgacttaa tgaacgtgca gtatcaatag tgatatcgcc aacggtgatc gttcccgctt
      661 ccggttgttc cagcagattt atgctacgta gcaatgtggt tttgccggaa ccactcggac
      721 cgataattgc caccacttcg ccaggcttta cctcgaggtc gataccgtgc agcaccgtct
      781 gaccgtggaa ttttttcact aggttcttaa cttcaatggc actcatttgg gttctctctc
      841 ctggcgatta agttgattct caaaatggtt ctgcaacgtc gataacacag tcgccatgat
      901 ccagtagatg agcgaagccg ccagatacat ggtgaatacc tccagcgtac gcgaggtaat
      961 caactgcgcc tgacggaaca gctccggcac ctggattgtc gcggccagcg acgtatcttt
     1021 taccagactg ataaagctgt tcgagagcgg cggcaacgcc acccgcgccg cctgtggcaa
     1081 aatcgcgcga cgcatagtct gccatggcgt cataccaata ctggcagctg cttcccactg
     1141 acctttatca atagaagaaa tggcggcgcg aagcgtttcg gcagtatagg cggcagtgtt
     1201 gagcgacaga ccaatcattg ctgacggaat gggatctaat tcgataccaa actgcggcag
     1261 gccgtaatag atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa tagagatata
     1321 aaaacgcgcc agccagcgca cgggccagat tggcgacagg cgcatcagcg cgagaataaa
     1381 ccctagcagt aaaccaaaaa acatgccgcc gatactgagt tgcagcgtat acccagcccc
     1441 tttcagcagg aacggcaagg aatcaataac cagttgtata ctttcttgca tgagcgttcc
     1501 cggagctaaa cgtggggatg ataggcgaac agcgcaggcg cgccgccggt atgaataaac
     1561 agaatcggcc cttcatcttt gaagcgtttc tgactgatac cgtcaatcag acccgccatc
     1621 gcttttccgg tatacacagg atcaagcaga atgccttcca gccgcgccag caatttcact
     1681 gcttccatgc cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc atcccagagt
     1741 aaaatttcca ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg taggttaacc
     1801 actttcggca attgatcggc aacggaacgc gacacggtca cgccaatcag ttcgctttca
     1861 ggcatcaggt gttccagccc aacagccagt ccggcgtgag ttccggcact gcccgatgcg
     1921 actaccaccg atgaaatatt aaccgcccct tcacactgtt gcgcgatttc cagcgcactc
     1981 tccacataac ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac atacgggcga
     2041 aagccttgtg cttcgactcg cgtcgccagc tcttccagtt gggcattggg atcggtcagt
     2101 gcgtcgcaca tttcaatctg ggtattgaac agatccagca acaagcgatt gccgttggtt
     2161 aaatagtttt ctgcggttgt gccaatagga ttttccagca gcgccacgca gtgcagaccg
     2221 agtttggcag cgactgcggc agtctgccgc acatggttag actggatcgc cccggcagta
     2281 atcagcgtat cggcaccttc acgcagagca tctgccgcga gaaattctag cttacgtaat
     2341 ttattgccgc ccattgccat gggggtgacg tcatcccgtt tgatgaaaat ttcccgtcct
     2401 agataatcag aaaagcgcgg cagatattcg agcggcgttg gcgcgccgat aaactccagc
     2461 cgtggaaaac gggttaaatt atgcagtggc ataacagcct ccgatgtgtg ttgttgtgat
     2521 tttctcatta tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa agcgcccttt
     2581 tttgtcatta tgctgattat ttggtcacat cagcaccaaa ccatttttcg gaaagggctt
     2641 gcaaagtgcc gtctttttgc atttccgcaa ttgcatcatt cactgctttc agcaggtctt
     2701 catttccttt acgcagcgcc acgccagact cctgacggga gaatgcttca ccggttactg
     2761 ccagcgtatc gttggttttc ttcaccagat ccagcgccgc cagacgatca acgaggatcg
     2821 catcgatacg ccctacgcgc agatcctgat atttggtcgg gtcatcatca taggtacgca
     2881 catcgacgcc ctgaacattc tgccgcagcc actcttcata gttggtgccc agaccgaccc
     2941 ccactttttt gcctttcaga tcatcggctg ttttaatggt gccttcgtta ccttttttca
     3001 ccagcgcctg aataccagaa atggtgtacg gggttgagaa atcgtatttt ttcttgcgct
     3061 catcagaaat ggtgacctga ttaatcacca catcaatacg tttagagtcc agcgacgcca
     3121 gcataccgtc ccatttggtc ggttttagtg acgcctcaac gccaagatgt tttgccagct
     3181 gttgggcaaa ttccacttca aaaccggtta atttgccgtc atccccctga aaactgaacg
     3241 gcggataagt accttccagc cctaccagca gcgtgccgcg ctctttaact ttattaagca
     3301 gaccttcatc tgcaaaactt ttaacgctca tgcccgcaat cagcgctacg gccatcacac
     3361 ccatcaatgc ctgacgtccc agatgtgcta atttcatatt caccccgaat gttgttatgt
     3421 ctgtttgcag tgtagagcca ttgtttgtaa acacaaaaac aactccgcta catcttattc
     3481 ttatttaata tatatcagaa gcaggcaggc tggaggattt ctgcacaagt ccggtacagg
     3541 tttggcgctg atagtgttga tacttccgta acgcgatgcg gtaattgttg gtgctggtgg
     3601 tgggcagcca cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga tcgagggaaa
     3661 tattttgctc gtgtagatga ttccccaaac ggcgcagacg aacgacatat tcacgcaccg
     3721 taccagggct catttcagtt tgctcaaaaa gaaattgctt aaagccgata atatcgaaaa
     3781 aatcactctg cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc gcccatgatt
     3841 tttgttccgt ttgccagcca ttttcatcaa gcagcgtgtc caggcgggaa atctcgattt
     3901 tattcactat tttgccgtcg cgaactaagg taatgcgatc gagcaattta cggcaatgcg
     3961 cgcaatgggt ctggctgtgt ttaaagtctt taagatagcg gcttaatggc cgtcttttca
     4021 ggtgctgcac catcattaag aactcctggt agtcaaagtt aaagtgcggc atttactgac
     4081 gttataactt acccagttta gtgcgtaacc gtttaatagc ctggctgtgt aactgactga
     4141 cccgcgattc cccgacctcc agcactgcac caatctcttt gagattcagc tcttcctggt
     4201 aatagagggt taataccagt ttttcgcgct ccggcaacgt ttcgatagct tccatcaccc
     4261 gctggcgcag attactgtcc agtagttgtt gtagcgggtt ttctcgttga tgatcatcag
     4321 taaccaattc gatgctatcg ccgtgctctt cgcgccactc atcgtaggag aagagctggc
     4381 tgttattggt gtcgagcaac atttggcgat aatcggcaat atcgatccct aaacgttccg
     4441 ctacctcagt ttccgtggcg ttgcggccaa gttcctgctc cagttgccct attgcctgtg
     4501 ccacttcacg cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg cgaagttcat
     4561 ccagcatagc gccacggata cgctgcactg cgtaagttgt aaatgccgtt ccttgtaggg
     4621 cgtcatagcg ttcgacggca ttaagtaacc caatgccgcc cgcctgtagc agatcgtcaa
     4681 gttccacgct cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc accagcggga
     4741 cataacgctg ccacagcgag tgtttatcca ttacaccttc agcggtatag agtgaattca
     4801 cgataaacag ccctgcgtta tatgagttat cggcatgatt atccgtttct gcagggtttt
     4861 taatcggacg attagtgggt gaaatgaggg gttatttggg ggttacaggt aaattccagg
     4921 cagaaaaaac cccgccggtg gcggggaagc acgttgctga caaattgcgc tttatgttgc
     4981 cggatgcggc gtaaacgcct tatccggcct acaagaatgt gcaaattcaa taaattgcaa
     5041 ttcaacttgt aggcctgata agcgcagcgc atcaggcaat ttggcgttgc cgtcagtctc
     5101 agttaatcag gttacaacga ttaaccctgc agcagagaca gaacctgctg cggtacctgg
     5161 ttagcttttg ccagcacgga gttaccggcc tgctgaatga tctgcgcttt cgacatgttg
     5221 gacacttcgg tcgcatagtc ggcgtcctga atacgggact gcgcttcgga caggttggta
     5281 gtggtgttgt tcaggttggt gactgcggaa tccagacggt tctggatagc acccagggaa
     5341 gaacggaatt tgtcgatgga gctgattgcg tcgtccaggg cagcaagcgg gttggtcgtt
     5401 gcagaaccag cactggtagt ctcagtagtg attttacccg cggagttcac atttacagca
     5461 gtaccaattg ctggagcata atctttattg gtatcagtcg ctgaagcata cccggcaaca
     5521 gtcacagagc cgttatcctt gttaacgctg taagagactg tatagtcggc aacgttagtg
     5581 ataccacctt tatcatccac ataagacttc gcagcatcac tggattcacc cgcggtaaac
     5641 ccaatagttt tgctcagtac accggaatta aaggtaattg aagatgtcgc ggcagccgct
     5701 ttggtttcgc tcaatactac atctttactc actgtagcct gataagtaat cccaccaggg
     5761 attaacggag ctactggagt ggttgtcgca ggagttgctt ttgcgatggt atattctgtg
     5821 ccattgaatg tcagagaggc accgtcacta ccttcgctcg ctgctttgag cagcgctttc
     5881 atatcagctt tagctgcgct accagcgttg ttagtcgtca agttgcctgc atcgtctacg
     5941 tatgcctggc ttccaccgat ggtgatatta cctgctgaat ccgtttcgaa agaaacagta
     6001 ccatcttttg tggtgtaaga accattaaca gatttaccca catcagcatg aagggtggca
     6061 gctaacgcat cacgttttga agctgaatca gcagcctgcg ccgcggcgtc tacacccgta
     6121 ccagcagtag atttagtggt agtaaaatca ccagatttag cgttgtaagt ataatctacg
     6181 ccgccaactg tgactttatc gccattccct aatttatcga atgcagcatc ggtagttaac
     6241 aaggtatttt cggttttcag atcataaaga cctgtcgtgg tgtttaactt cgcgccagca
     6301 gaagttaaat cacttaccgt tgcagctttg ttggtaatag tacctttacc atttacgtta
     6361 aagccattca gacccagagt atcagaatcg atttttttca ggtcgatcgt gatggtttcg
     6421 ccgtcattcg caccaacctg aattttcatt gaaccgtctt tcgccagcac gttcacgccg
     6481 ttgaactggg tctggccgga tacgcggtca atttcatcaa gacgagattt gatttcgtcc
     6541 tggatggagt ccaggtcaga atcggagtta gtccctgtag tggcctgaac cgtcagttca
     6601 cgaatacgct gtaagttgtt gttgatttcg gacagcgcgc cttcggtggt ctgcgcaaca
     6661 gaaataccgt cgttggcgtt acgggccgcc tgagtcaggc ctttaatgtt agaagtaaaa
     6721 cggttagcaa tcgcctgacc tgcggcgtca tccttcgcgc tgttaatacg caagccagaa
     6781 gacagacgct cgatagaact cgacagcgca gactggttct tgttgatatt attttgagtg
     6841 atcagcgaga ggctgttggt attaatgact tgtgccatga ttcgttatcc tatattgcaa
     6901 gtcgttgatt acgtattggg tttccacccg tcggctcaat cgccgtcaaa cctgttatcg
     6961 tctgtcgtaa aacaaccttt agaatttttt tcacaaacag ccattttttg ttagtcgccg
     7021 aaatactctt ttctctgccc cttattcccg ctattaaaaa aaacaattaa acgtaaactt
     7081 tgcgcaattc aggccgataa ccccggtatt cgttttacgt gtcgaatgat aaaaggaaat
     7141 cgcatggcaa gtatttcatc gctgggagtc gggtcaggtc tggatttaag ttcaatcctt
     7201 gatagcctca ccgccgcgca aaaagcgacg ctaaccccca tttcaaatca gcaatcgtcg
     7261 tttaccgcta aacttagcgc ctacggtacg ctgaaaagcg cgctgacgac tttccagacc
     7321 gccaatactg cattgtctaa agccgatctt ttttccgcca ccagcagcac caccgcgttc
     7381 agtgccacca ccgcgggtaa cgccatcgcc gggaaataca ccatcagcgt cacccatctg
     7441 gcgcaggcgc aaaccctgac cacgcgcacc accagagacg atacgaaaac ggcgatcgcc
     7501 accagcgaca gtaaactcac cattcaacaa ggcggcgaca aagatccgat taccattgat
     7561 atcagcgcgg ctaactcgtc gttaagcggg atccgtgatg ccatcaacaa cgcaaaagca
     7621 ggtgttagcg cgagcattat taacgtgggt aacggtgatt atcgtctgtc agtcacatca
     7681 aatgacaccg gccttgacaa tgcgatgacg ctctcggtta gcggtgatga tgcgctacaa
     7741 agttttatgg gctatgacgc cagtgccagc agcaacggca tggaggtctc ggtcgccgcc
     7801 cagaatgcgc agctgacggt caacaacgtc gccattgaga acagcagcaa caccatcagc
     7861 gacgcgctgg aaaacatcac cctgaacctg aacgatgtca ccacgggcaa ccagacgcta
     7921 accatcactc aggacacctc caaagcgcaa acggcgatta aagattgggt gaatgcctat
     7981 aactcgctaa tagatacctt cagcagcctg accaaataca ccgccgtaga tgcgggagct
     8041 gatagccaga gttctagcaa tggcgcactg ctcggcgact ccacgctgcg gacgattcag
     8101 acgcagttga agtcgatgct gagtaatacc gtcagttctt ccagctataa aacgttggcg
     8161 cagattggta tcacgaccga tcccagcgat ggcaaactgg aactggatgc cgacaaactc
     8221 accgctgcac tgaaaaaaga tgccagcggc gtaggtgcat tgattgttgg cgatggtaaa
     8281 aaaaccggca tcacgaccac catcggcagc aacctgacca gttggctttc gacaacgggc
     8341 attattaaag ccgctaccga tggcgttagt aagaccctga ataaattaac taaagactac
     8401 aacgccgcca gcgatcgcat tgacgcgcag gtcgcgcgct acaaagaaca atttacccaa
     8461 ctggacgttt taatgacctc gttaaacagc acaagcagct acttaacgca gcagttcgaa
     8521 aacaacagta attccaagta atcattattc atcgggagac aggtcatgta cgcggcaaaa
     8581 ggcacccaag cctatgcaca aattggcgtc gaaagcgccg taatgagcgc cagccagcag
     8641 cagctggtca ccatgctatt tgatggagtg ctgagcgcac tggttagagc gcgcctgttt
     8701 atgcaggaca acaatcagca aggcaaaggc gtctctttgt caaaagcgat caacatcatt
     8761 gagaacggac tgcgggtgag tcttgatgaa gagagcaaag acgaactaac ccaaaacttg
     8821 attgctcttt atagctatat ggtcaggcgt ttgctgcaag ccaatttacg caacgatgtc
     8881 tccgcagtcg aagaagtgga aacattaatg cgcaatattg ccgatgcctg gaaagagtcg
     8941 ttactctccc cttctttgat tcaggaccca gtctgatgaa ccatgcaccg catttatatt
     9001 tcgcctggca acaactcgtc gaaaaaagcc agctcatgtt acgcctggca acggaagaac
     9061 aatgggacga actcatcgcc agcgaaatgg cgtatgttaa tgcggtgcag gagattgcac
     9121 atttgactga agaggttgac ccgtccacca cgatgcagga gcagctccgc ccgatgctgc
     9181 gcctgattct cgacaacgaa agcaaggtaa agcagttatt acagattcgg atggatgaac
     9241 tggcgaaact ggtcggtcag tcatcggtgc aaaaatcggt gttgagtgcc tatggcgatc
     9301 agggcggctt tgtgctggct ccgcaggata acctcttttg aatctgaatg agtcgatggc
     9361 tcgcgaataa tccgattacg gctacgcttc taatgttccc cttgaatgga gtcgaagaat
     9421 gcgtaatccc acgctgttac aatgttttca ctggtattac ccggaaggcg gtaagctctg
     9481 gcctgaattg gccgagcgcg ccgacggttt taatgatatt ggtatcaata tggtctggtt
     9541 gccgcccgcc tataaaggcg catcgggcgg gtattcggtc ggctacgact cctatgattt
     9601 atttgattta ggtgagtttg atcagaaagg cagcatccct actaaatatg gcgataaagt
     9661 acaactgctg gccgccattg acgccctgaa acgtaatgac attgcggtgc tgttggacgt
     9721 ggtagtcaac cacaaaatgg gcgcggatga aaaagaagct attcgcgtgc agcgcgttaa
     9781 tgctgatgac cgtacgcaaa ttgatgaaga aatcattgaa tgcgaaggct ggacgcgtta
     9841 caccttcccc gctcgtgccg ggcaatactc acagtttatc tgggatttca aatgttttag
     9901 cggtatcgac catatcgaaa accccaatga agatggcatt tttaaaattg ttaacgacta
     9961 caccggcgaa ggctggaacg atcaggttga tgatgaactc ggtaatttcg attatctgat
    10021 gggcgagaat atcgattttc gcaatcatgc cgtgacggaa gagattaaat actgggcgcg
    10081 ctgggtgatg gaacaaacgc aatgcgacgg ttttcgtctt gatgcggtca aacatattcc
    10141 agcctggttt tataaagagt ggatcgaaca cgtacaggaa gttgcgccaa agccgctgtt
    10201 tattgtggcg gagtactggt cgcatgaagt tgataagctg caaacgtata ttgatcaggt
    10261 ggaaggcaaa accatgctgt ttgatgcgcc gctgcagatg aaattccatg aagcatcgcg
    10321 catgggacgc aactacgaca tgacgcagat tttcactggg acgctggtgg aagccgatcc
    10381 ttttcacgcc gtaaccctcg ttgccaacca cgacacccaa ccgttgcaag ccctcgaagc
    10441 accggtcgaa ccgtggttta aaccgctggc gtatgctcta attttgttgc gggaaaatgg
    10501 cgttccttcg gtgttctatc cggacctcta cggtgcgcat tacgaagatg tcggtggtga
    10561 cgggcaaacc tatccgatag atatgccaat aatcgaacag cttgatgagt taattctcgc
    10621 ccgtcagcgt ttcgcccacg gtgtacagac gttatttttc gaccatccga actgcattgc
    10681 ctttagccgc agtggcaccg acgaatatcc cggctgcgtg gtggtcatgt cgaacggaga
    10741 tgatggcgaa aaaaccattc atttaggaga gaattacggc aataaaacct ggcgtgattt
    10801 tcttgggaac cggcaagaga gtgtagtaac cgacgaaaac ggcgaagcga ccttcttttg
    10861 caacggcggc agcgttagcg tgtgggttat cgaagaggtg ctttaaattc atccccggcg
    10921 gcaagccggg gagatttcat tacggcaatg gcgtcggtaa cggcgcttta tccagcgcag
    10981 cggcacactc tgccgtcggg cgatccacac gctccatcgt cataccatcg tactcaatgg
    11041 tgttaccgtc acgctcaacc tcgtacagct cacgttttac ggtaacgtta gtgagatcat
    11101 cagacatcaa tgtcaacttg cccggcacag caatcacccg ctgccactga cggcaatcta
    11161 gggtatcgcc ctctttagtg acaatcaggc tgcctatcgc ttccgggctg accagcgcac
    11221 gttgcggccc tttggtctgc cagtagcccg ccagccagtc cggcgcgggg gttttcacga
    11281 cattgttata gttttcgact tcggcacagc ccgccagcaa catgagcgcg cctgcaattg
    11341 cgagtttttt catcattcgt cctgcatgag aagaaaagag gattgtggca ttaaagccct
    11401 gatgtcgcca gcctttccag gttcagagag aaatttgatc taactattat ttttgtgaat
    11461 tattaccccg aaaataaatt tgcatggtgt atgattcgcg actcttgttt caaaccttct
    11521 gagttcagag gctacattca tgtcatggca gcaattcaaa cacgcctggt tgattaaatt
    11581 ctgggcgccc atccccgcgg tcatcgcggc gggtattctc tccacttact attttggcat
    11641 cactggcacc ttttgggctg tcacgggtga atttacccgt tggggcggtc agctcctgca
    11701 gctgttcggc gtccatgctg aagagtgggg ttattttaaa attatccatc tggaaggatc
    11761 gccattaacc cgcatcgacg ggatgatgat cctcggtatg tttggcggct gctttgccgc
    11821 agcgctgtgg gccaacaatg tcaaactacg catgccgcgt agccgtatcc gcattatgca
    11881 ggccattatt ggcggcatta tcgccggttt tggcgcgcgt ctggcaatgg gctgtaacct
    11941 ggcggcattc tttaccggta ttcctcagtt ctcgctgcat gcctggttct ttgccatcgc
    12001 cactgccatt ggttcatggt ttggcgcgcg ctttaccctg ctgcccatct tccgtattcc
    12061 cgtgaaaatg cagaaagttg ctgccgcctc accgctgacg caaaaaccgg atcaggcgcg
    12121 gcgtcgtttt cgtctcggga tgctggtctt tttcggcatg ctgggctggg cgctgctcac
    12181 agcgatgaac caacccaaac tggggctggc aatgctgttt ggcgtcggct ttggtttact
    12241 gattgaacgt gcgcaaatct gctttacttc ggcgttccgc gatatgtgga tcaccggacg
    12301 tacccatatg gcgaaagcaa tcattattgg tatggcggtg agtgccatcg ggatcttcag
    12361 ttacgtacag ttaggcgttg aacccaaaat catgtgggcg ggaccaaacg cggtaattgg
    12421 tggtttactg tttggttttg gcatcgtgct ggctggcggc tgcgaaaccg gctggatgta
    12481 ccgcgcggta gaaggccagg tgcactactg gtgggtcggt ctgggcaatg tgatcggctc
    12541 aacgattctg gcgtattact gggatgattt cgctccggcg ctggccaccg actgggacaa
    12601 aatcaacttg ctgaaaacct ttggtccgat ggggggcctg ctggtgacat atttgctgtt
    12661 gtttgctgcg ctgatgttga ttatcggctg ggaaaaacgc ttcttccgcc gtgcggcacc
    12721 gcagactgct aaggagatcg catgaaaaat atcgttcctg attaccgcct tgatatggtg
    12781 ggtgaaccct gcccttatcc ggcagtcgca acccttgagg cgatgccgca gttaaaaaaa
    12841 ggggaaatcc tggaagtggt gagcgactgt ccgcagtcga tcaataatat tccactggat
    12901 gcgcgtaatc acggctatac ggtgctggat attcagcaag acgggccgac cattcgttat
    12961 ttaattcaga agtaatccct cattcccgct gtgacccaca gcgggaacat cgttaccttt
    13021 gtatcacata gattttaccc gccccctctc ctattctttt cagtatgatc tcaggaggtt
    13081 tctatgtgtg gacgctttgc ccaatcccaa acgcgtgaag attaccttgc gcttctcgcg
    13141 gaagatattg aacgcgatat tccctatgat cccgaaccca ttggcagata caacgtcgcg
    13201 ccgggaacca aagtcctact gctcagtgaa cgtgatgaac accttcatct ggatccggtt
    13261 ttctggggat atgctcccgg atggtgggat aaaccgccgc tgattaacgc ccgcgtagaa
    13321 actgcggcaa ccagtcgtat gtttaaaccg ctctggcaac atggtcgggc aatctgtttt
    13381 gccgatggct ggtttgagtg gaaaaaagaa ggcgacaaaa agcagcctta ttttatctat
    13441 cgtgctgatg gacaacctgt ttttatggca gcgataggca gcacaccgtt cgagcgcggt
    13501 gacgaagccg aaggattttt gattgtcact gcagcagcag accagggtct ggtagatatt
    13561 catgaccgcc gcccgctggt tctgtcgcca gaagctgcgc gggaatggat gcggcaagag
    13621 attagcggta aagaagcctc agaaatcgcg gccagtggct gtgttccagc aaaccagttc
    13681 tcctggcacc cggtatcgcg cgcggtgggt aatgttaaaa accagggtgc ggagttaatt
    13741 caacctgttt gataagcctg gagattattg atcaacaata ctgcgtcata agtaatctct
    13801 attagacaaa gatttcatta cctgttggca tattgcaaaa ataacaccaa tacggaatcg
    13861 tcatgttcac gattaaaaca gatgatctca cccatccagc agtgcaagca ttagtggctt
    13921 accatatttc cggcatgctg cagcagtctc cccctgaaag cagtcatgct ttagacgtgc
    13981 aaaaattacg taacccgaca gtgacattct ggtcagtatg ggaaggcgaa caactcgcag
    14041 gaattggtgc gctgaagttg ctggatgata aacatggcga actgaaatca atgcggaccg
    14101 cgccaaatta tttacgtcgg ggtgtcgcca gtctgatttt acgccacatt ttgcaggtcg
    14161 cccatgacag atgccttcat cgcctgagtt tagaaacggg tacacaggct ggatttacgg
    14221 cctgccatca actttatttg aagcatggtt tcgttgattg cgaaccgttt gccgattatc
    14281 aacttgatcc acacagtcga tttttgtcat tgacgctatg cgaagataat gagttgcttt
    14341 gagccagacg cagcacattc
//
LOCUS       DABSYT020000109        14342 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.109, whole genome shotgun sequence.
ACCESSION   DABSYT020000109 DABSYT020000000
VERSION     DABSYT020000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.109"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            111..4073
                     /gene="putA"
                     /locus_tag="ID725_RS19735"
                     /old_locus_tag="pgaptmp_003947"
     CDS             111..4073
                     /gene="putA"
                     /locus_tag="ID725_RS19735"
                     /old_locus_tag="pgaptmp_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGV7852438.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(4113..4751)
                     /gene="rutR"
                     /locus_tag="ID725_RS19740"
                     /old_locus_tag="pgaptmp_003948"
     CDS             complement(4113..4751)
                     /gene="rutR"
                     /locus_tag="ID725_RS19740"
                     /old_locus_tag="pgaptmp_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGV7852439.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            5039..6130
                     /gene="rutA"
                     /locus_tag="ID725_RS19745"
                     /old_locus_tag="pgaptmp_003949"
     CDS             5039..6130
                     /gene="rutA"
                     /locus_tag="ID725_RS19745"
                     /old_locus_tag="pgaptmp_003949"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGV7852440.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            6130..6822
                     /gene="rutB"
                     /locus_tag="ID725_RS19750"
                     /old_locus_tag="pgaptmp_003950"
     CDS             6130..6822
                     /gene="rutB"
                     /locus_tag="ID725_RS19750"
                     /old_locus_tag="pgaptmp_003950"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGV7852441.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            6834..7173
                     /gene="rutC"
                     /locus_tag="ID725_RS19755"
                     /old_locus_tag="pgaptmp_003951"
                     /pseudo
     CDS             6834..7173
                     /gene="rutC"
                     /locus_tag="ID725_RS19755"
                     /old_locus_tag="pgaptmp_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
     gene            7181..7981
                     /gene="rutD"
                     /locus_tag="ID725_RS19760"
                     /old_locus_tag="pgaptmp_003952"
     CDS             7181..7981
                     /gene="rutD"
                     /locus_tag="ID725_RS19760"
                     /old_locus_tag="pgaptmp_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGV7852442.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            7991..8581
                     /gene="rutE"
                     /locus_tag="ID725_RS19765"
                     /old_locus_tag="pgaptmp_003953"
     CDS             7991..8581
                     /gene="rutE"
                     /locus_tag="ID725_RS19765"
                     /old_locus_tag="pgaptmp_003953"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGV7852443.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            8592..9086
                     /gene="rutF"
                     /locus_tag="ID725_RS19770"
                     /old_locus_tag="pgaptmp_003954"
     CDS             8592..9086
                     /gene="rutF"
                     /locus_tag="ID725_RS19770"
                     /old_locus_tag="pgaptmp_003954"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGV7852444.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQN
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            9107..10435
                     /gene="rutG"
                     /locus_tag="ID725_RS19775"
                     /old_locus_tag="pgaptmp_003955"
     CDS             9107..10435
                     /gene="rutG"
                     /locus_tag="ID725_RS19775"
                     /old_locus_tag="pgaptmp_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGV7852445.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(10518..10625)
                     /locus_tag="ID725_RS19780"
                     /old_locus_tag="pgaptmp_003956"
     CDS             complement(10518..10625)
                     /locus_tag="ID725_RS19780"
                     /old_locus_tag="pgaptmp_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HGV7852446.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            10997..11593
                     /gene="wrbA"
                     /locus_tag="ID725_RS19785"
                     /old_locus_tag="pgaptmp_003957"
     CDS             10997..11593
                     /gene="wrbA"
                     /locus_tag="ID725_RS19785"
                     /old_locus_tag="pgaptmp_003957"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGV7852447.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMS
                     PQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            11614..11841
                     /gene="yccJ"
                     /locus_tag="ID725_RS19790"
                     /old_locus_tag="pgaptmp_003958"
     CDS             11614..11841
                     /gene="yccJ"
                     /locus_tag="ID725_RS19790"
                     /old_locus_tag="pgaptmp_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGV7852448.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(11879..13120)
                     /gene="agp"
                     /locus_tag="ID725_RS19795"
                     /old_locus_tag="pgaptmp_003959"
     CDS             complement(11879..13120)
                     /gene="agp"
                     /locus_tag="ID725_RS19795"
                     /old_locus_tag="pgaptmp_003959"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGV7852449.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(13411..14342)
                     /locus_tag="ID725_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /pseudo
     CDS             complement(13411..14342)
                     /locus_tag="ID725_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526794.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3301 a         3753 c         3820 g         3468 t
ORIGIN      
        1 tttaagcttg ctacgcaggt cacatttaac atggttgcac aaagttgcaa catcatggat
       61 atttcacgat aacgttaagt tgcacctttc agaacaacag gagtaatggc atgggaacca
      121 ccaccatggg ggttaagctg gacgacgcga cgcgtgagcg tattaagtct gccgcgacac
      181 gtatcgatcg cacgccacac tggttaatta agcaggcgat tttttcttat ctcgaacaac
      241 tggaaaacag cgatactctg ccagagctac ctgcgctgct ttctggcgct gccaatgaga
      301 gcgatgaagc accgactccg gcagaggaac cacaccagcc attcctcgac tttgccgagc
      361 aaattttgcc ccagtcggtt tcccgcgctg cgatcaccgc ggcctatcgc cgcccggaaa
      421 ccgaagcggt ttctatgctg ctggaacaag cccgcctgcc gcaaccagtt gctgaacagg
      481 cacacaaact ggcgtatcaa ctggccgata aactgcgtaa tcaaaaaaat gccagtggcc
      541 gcgcaggtat ggtccagggg ttattgcagg agttttcgct gtcatcgcag gaaggcgtgg
      601 cgctgatgtg tctggcagaa gcgttgttgc gtattcccga caaagccacc cgtgatgcgt
      661 taattcgcga caaaatcagc aacggtaact ggcagtcaca cattggtcgt agcccgtcac
      721 tgtttgttaa tgccgctacc tgggggctgt tgtttactgg caaactggtt tccacccata
      781 acgaagccag cctctcccgc tcgctgaacc gcattatcgg taagagcggt gaaccgctga
      841 tccgcaaagg tgtggatatg gcaatgcgcc tgatgggcga gcagttcgtc acgggcgaaa
      901 ccatcgcgga agcgttagcc aatgcccgca agctggaaga gaaaggtttc cgttactctt
      961 acgatatgct cggcgaagct gcactgactg ccgcagacgc acaggcgtac atggtttcct
     1021 atcagcaggc gattcacgcc attggtaaag cgtctaacgg tcgtggcatc tatgaaggtc
     1081 cgggcatttc aatcaagctg tcggcgctgc atccgcgtta cagccgcgcc cagtatgacc
     1141 gggtaatgga agagctttac ccgcgtctga aatcactcac cctgctggcg cgtcagtacg
     1201 atattggtat caacattgac gccgaagagg ccgatcgtct ggagatctcc ctcgatctgc
     1261 tggaaaaact ctgtttcgag ccagagctgg caggctggaa cggcatcggt tttgttattc
     1321 aggcttatca aaaacgctgc ccgctggtga ttgattacct gattgatctc gccacccgca
     1381 gccgtcgccg tcttatgatt cgcctggtga aaggcgcgta ctgggatagt gaaatcaagc
     1441 gcgcgcagat ggacggcctt gaaggttatc cggtttatac ccgcaaggta tataccgacg
     1501 tttcttatct cgcctgtgcg aaaaagctgc tggcggtgcc gaatctaatc tacccgcagt
     1561 tcgcaacgca caacgcccac acgctggcgg cgatttatca actggcaggt cagaactact
     1621 atccgggtca gtacgagttc cagtgcctgc atggtatggg cgagccgctg tatgagcagg
     1681 tcaccgggaa agttgccgac ggcaaactta accgtccgtg tcgtatttat gccccggtcg
     1741 gcacacatga aacgctgctg gcgtatctgg tgcgccgcct gctggaaaac ggtgctaaca
     1801 cctcgtttgt taaccgtatt gccgatacct ctttgccact ggatgaactg gtcgccgatc
     1861 cggtcactgc tgtagaaaaa ctggcacaac aggaagggca aactggatta ccgcatccga
     1921 aaattcccct gccgcgcgat ctttacggtc acgggcgcga caactcggca gggctggatc
     1981 tcgctaacga acaccgtctg gcatccctct cttctgccct gctcaatagt gcactgcaaa
     2041 aatggcaggc cttgccaatg ctggaacaac cggtagcggc aggtgagatg tcgcccgtta
     2101 ttaacccggc ggaaccgaaa gatattgtgg gctatgtacg tgaagccacg ccgcgtgaag
     2161 tagagcaggc gctggaaagt gcggttaata acgcgccaat ctggtttgcc actcctccgg
     2221 ctgaacgcgc ggcgattttg catcgcgctg ccgtgctgat ggaaagccag atgcagcaac
     2281 tgattggtat tctggtgcgt gaagccggaa aaaccttcag taacgccatt gccgaagtgc
     2341 gtgaagcggt cgattttctc cactactacg ccggacaggt gcgggatgat ttcgctaacg
     2401 aaacccatcg tccattaggg cctgtggtat gtatcagccc gtggaacttc ccgctggcga
     2461 ttttcaccgg gcagatcgct gccgcactgg cggcaggtaa cagcgtactg gcaaaaccgg
     2521 cagaacaaac gccactgatt gccgcgcaag ggatcgccat tttgctggaa gcgggcgtac
     2581 cgccaggcgt ggtgcaattg ctgccaggtc ggggtgaaac cgtgggcgcg caactgacgg
     2641 gtgatgatcg cgtacgcggg gtgatgttta ccggttcaac cgaagtcgct acgttactgc
     2701 agcgcaatat cgccagccgc ctcgacgctc agggtcgccc tattccgctc atcgctgaaa
     2761 ccggcggcat gaacgcgatg attgtcgatt cttcagcact gaccgaacaa gtcgtaatag
     2821 atgtgctggc ctcggcgttc gacagtgcgg gtcagcgttg ttcggcgctg cgcgtgctgt
     2881 gcctgcaaga tgagattgcc gaccacacgt tgaaaatgct gcgcggcgca atggccgaat
     2941 gccggatggg taatccgggt cgcctgacca ccgatatcgg tccggtgatt gatagcgaag
     3001 cgaaagccaa tattgagcgc catattcaga ccatgcgtag caaaggccgt ccggtgttcc
     3061 aggcggtgcg ggaaaacagc gaagatgccc gtgaatggca aagcggcacc tttgtcgccc
     3121 cgacgctgat cgaactggat gactttgccg aattgcaaaa agaggtcttt ggtccggtgc
     3181 tgcatgtggt gcgttataac cgtaatcagc tgccggagct gatcgagcag attaacgctt
     3241 ccggctatgg tctgacgctt ggcgttcata cgcgtattga tgaaaccatc gcccaggtca
     3301 ctggctcagc ccatgttggt aacctgtatg ttaaccgtaa tatggtgggc gcagtggttg
     3361 gggtgcagcc attcggcggc gaagggttgt ccggtaccgg gccgaaagca ggcggtccgc
     3421 tctatctcta ccgtctgctg gcgaatcgcc cggaaagtgc gctggcggtg acactcgcgc
     3481 gtcaggatgc agagtatccg gtcgatgcgc agttgaaagc cgcattgact cagccgctaa
     3541 atgcactgcg ggaatgggca gcaaatcgtc cagaattgca ggcgttatgt acgcaatatg
     3601 gcgagctggc gcaggcagga acacaacgat tgctgccagg gccgacgggt gagcgcaaca
     3661 cctggacgct gctgccgcgt gagcgcgtat tgtgtatcgc ggatgatgaa caggatgcgc
     3721 tgactcagct cgccgccgtg ctggcggtgg gcagccaggt gctgtggcca gatgacgcgc
     3781 tgcatcgtca gttagtgaag gcattgccat cgactgtcag cgagcgtatt caactggcga
     3841 aagcggaaaa tattaccgct caaccgtttg atgcggtgat cttccacggt gattcggatc
     3901 agcttcgcgc attgtgtgaa gcagttgccg cgcgggatgg cgcaattgtt tcggtgcagg
     3961 gttttgcccg tggcgaaagc aatatcctgc tggaacggct gtatatcgag cgttcgctga
     4021 gtgtgaatac cgctgccgct ggcggtaacg ccagcttaat gacgatcggt taaacaggta
     4081 gccggaggat gttacaccct cctccggcat cattagcgtg gtcgaatccc ctcaataata
     4141 atccgctgca cgttttcaac cgtttgattg aaaaatacct catcgcgcaa cgttgcgcct
     4201 gtcaccgcct ccacctgagg ggcgaaatcg gcgtaatgtt gagtggaagc ccaaatcata
     4261 aaaatcaaat gctgcggatc aatcggcgcg agtttgccgc ttttgaccca accggcaatc
     4321 agcgccgatt tctcatcaat taatgacttc aaatcgcccg tcagttcatc cattaacagc
     4381 ggcgcgcctg ccagcatctc catacagaac aggcgcgaag cctgcggata atcgcgtgag
     4441 acttccagct tcagacggat gtactctttg atcgccgcca gcggggcgaa atcttcacga
     4501 aacgctttta acggtgccag ccagatatcg agaatctgcc gcagcacggc aatatacagc
     4561 gcctcttttg acggaaagta atacagcaga ttggtttttg aaacacccgc caactctgca
     4621 atttgctcca gccgtgtgcc gtgaaaaccg aattgtgaaa aagtgtccag tgctgcgcta
     4681 agaatcgctt ttttcttcgc gcttactgcg cgcgaacgtt tacccgttgt tttcactgcg
     4741 ccttgcgtca tgcgctctcc cctcttgtag attggcaaca ggatagcaaa acccccgccc
     4801 ggcctcgacc ttctgcactc tcatcgcgca cagatgcatg ttttatgtgc aactgttttg
     4861 accgtttagt ccacttttta ccagacattt tagataacgc gatgttaaca cattaataac
     4921 actccctttt taaaaactgg catccgcttt gcaaacaagc caatacgcgg ctgaaaagga
     4981 tatgcagaat gcagcgcccc gtctgacttt cactttacga gacgaagaga ggttgatgat
     5041 gaaaattggc gtattcgtac ctattggcaa caacggctgg ctcatttcga cccacgcgcc
     5101 gcagtacatg ccgacctttg aactgaataa agccatcgtg caaaaagcgg agcactacca
     5161 tttcgatttc gccctgtcga tgatcaaact gcgtggcttt ggcggcaaaa ctgagttctg
     5221 ggatcacaac cttgagtcgt tcaccttgat ggcggggctg gcggccgtga cctcgcgcat
     5281 tcagatttac gccaccgccg ccaccttaac gttacctccg gcaatcgtcg cccgtatggc
     5341 cgcaaccatc gactccatct ctggcgggcg ttttggcgtc aacctcgtga ctggctggca
     5401 aaagcccgag tatgagcaga tgggtatctg gcctggcgat gactatttct cccgtcgtta
     5461 cgactatctc accgagtatg ttcaggtgct gcgcgacctg tggggcacgg gaaaaagcga
     5521 ttttaaaggc gattttttca ccatgaatga ttgtcgcgtc agtccgcaac cgagtgtccc
     5581 catgaaagtg atctgcgccg ggcaaagcga cgctggcatg gcgttctccg ctcagtatgc
     5641 cgatttcaac ttctgtttcg gcaaaggcgt aaatacaccc acggctttcg ccccgaccgc
     5701 tgcgcggatg aaacaggccg cagagcaaac cgggcgcgac gttggctctt atgtattgtt
     5761 tatggtgatt gccgatgaaa ccgacgatgc cgctcgcgcc aaatgggaac actacaaagc
     5821 gggcgcggat gaagaggcgt taagctggct aaccgaacaa agtcagaaag atacccgctc
     5881 cggtactgac accaacgtcc gtcagatggc cgatcccact tcggcggtaa acatcaatat
     5941 ggggacgtta gtcggttctt acgccagtgt cgcgcgcatg ttagatgaag tcgcaagcgt
     6001 gcctggtgcc gaaggcgtgc tgttaacctt cgacgatttt ctgtcgggaa tcgaaacctt
     6061 cggcgagcgc attcaaccac tgatgcagtg ccgcgcccat ctccctgtgc tgactcagga
     6121 ggtggcatga tgacgacatt aaccgctcga ccggaagcca ttaccttcga tccgcagcaa
     6181 agtgcgctga tcgtggtgga tatgcaaaac gcctatgcca cgccaggcgg ctacttagat
     6241 ctcgccgggt ttgatgtctc aaccactcgc ccggtcattg ccaacattca aaccgccgtg
     6301 accgcagcgc gagcggcagg gatgttgatc atctggtttc aaaatggctg ggatgaacag
     6361 tatgtcgagg ctggcggccc tggctcaccg aattttcata aatcgaacgc cctcaaaacc
     6421 atgcgtaaac agccgcagct gcaggggaaa ttgctggcga aaggctcctg ggattatcaa
     6481 ctggtggatg aactggtgcc gcagcctggc gatattgtgc tgccgaagcc gcgctacagc
     6541 ggtttcttca atacgccgct ggacagcatt ttgcgcagcc gcggaatacg ccatctggtt
     6601 ttcaccagca tcgctaccaa cgtctgcgtc gaatcgacgc tacgcgatgg cttttttctg
     6661 gagtatttcg gcgtggtgct ggaagacgca acacaccagg cggggccgga atttgtacag
     6721 aaagccgcgt tgttcaatat cgaaaccttt tttggctggg tcagcgacgt cgaaacgttc
     6781 tgcgacgcgc tttctcccac gtcctttgct cgtatcgctt aaggagttta acgatgccaa
     6841 aatccgtaat tattcccgct ggcagcagcg caccgctggc ccccttcgtt cccggcacgc
     6901 tggctgatgg cgtggtgtat gtctccggta cgctggcttt tgatcaacac aataacgtgc
     6961 tgtttgccga tgacccaaag gcgcaaaccc gccacgttct ggaaactatc cgcaaggtaa
     7021 tcgagacagc gggtggcacg atggcggatg tgaccttcaa cagcatcttt attaccgact
     7081 ggaaaaatta cggcgcgatt ctgcattcag tgcggactgg taaaacctga cgcgctggtg
     7141 gaaatcgcca caattgcgca tatcgccaag tgaggccgcg atgaaacttt cactctcacc
     7201 tcccccttat gctgatgcgc ccgtagtggt gttgatttcg ggtcttggcg gtagcggcag
     7261 ttactggtta ccgcaactgg cggtgctgga gcaggagtat caggtagtct gttacgacca
     7321 gcgcggcacc ggcaataatc ccgacacgct ggcagaagat tacagtatca cccagatggc
     7381 agcggaactg catcaggcgc tggtagccgc agggattgag cattacgcag tggtcggcca
     7441 tgcgctcggt gcgctggtgg gaatgcagct ggcgctggat catcccgcgt cggtaactgt
     7501 gctggtctgc gttaacggct ggctacgaat aaacgcccat acgcgccgct gttttcaggt
     7561 tcgcgaacga ttactgtata gcggtggcgc gcaggcatgg gtggaagcgc agccgttgtt
     7621 cctctatccc gccgactgga tggcggcccg cgcacctcgc cttgaggcag aagacgcgct
     7681 ggcactggcg cattttcagg gcaaaaataa tttactgcgt cgacttaacg ccctcaaacg
     7741 cgctgacttt agtcaccatg cggatcgcat ccgctgcccg gtgcaaatca tctgcgccag
     7801 tgatgatctg ctggtgccat cagcatgttc cagtgaactt catgccgccc tgcccgatag
     7861 ccagaaaatg gtgatgcgct atggcggaca cgcctgcaac gtgaccgatc ccgaaacgtt
     7921 taatgctctg ttactcaacg ggcttgccag cctgttacat caccgtgaag ccgccctgta
     7981 aggaattgct atgaacgaag ccgttagccc aggtgcgctt agcaccctgt tcaccgatgc
     8041 ccgcactcac aacggctggc gggagacacc cgtcagcgat gagacgttac gggagcttta
     8101 tgccctgatg aaatgggggc cgacatcagc taactgttct ccggcacgta tcgtgtttat
     8161 ccgcactgca gagggaaaag aacgtctgcg cccggcgctt tccagcggca atctgcaaaa
     8221 aaccctgacc gcgcccgtca ccgctatcgt cgcatgggac agtgaatttt atgaacggtt
     8281 accgcaactg tttcctcacg gcgatgcccg cagttggttt acctccagcc cacaacttgc
     8341 cgaagaaaca gcgtttcgca acagttccat gcaggcggct tatctgatca tcgcctgccg
     8401 tgcgctgggg ctggataccg gcccgatgtc gggttttgat cgtcaacacg tagacgacgc
     8461 tttttttgca ggcagcacgc tgaagagcaa tctgctgatt aatatcggct atggcgatag
     8521 cagcaaactt tttgcgcgcc tgccacgtct gtcctttgaa gaagcctgcg ggctgttgta
     8581 aggagcaaac tatgaacatt gtcgatcaac aaacttttcg cgatgcgatg tcctgcatgg
     8641 gcgcggcggt caatatcatc accacggacg gtccagccgg gcgcgccggg ttcaccgcca
     8701 gcgccgtctg cagcgtgacc gacacgcctc ccacattact ggtgtgcctg aatcgtgggg
     8761 cgtccgtctg gccggtattc aatgaaaatc gaacgctgtg tgtaaataca cttagcgccg
     8821 ggcaggagcc gctttcaaac ctttttggcg gcaaaacgcc catggaacac cgctttgccg
     8881 ccgcccgctg gcagaatggt gtgaccggat gcccgcaact ggaagaggcg ctggtttcgt
     8941 ttgactgccg tatcagccag gtggtgagcg tcggcaccca cgacattttg ttttgcgcca
     9001 tcgaagcgat tcatcgtcac gccaccccct acgggctggt gtggttcgat cgcagttatc
     9061 acgcgctgat gcgccctgct tgttaacctc cttaaggaga cagctgatgg caatgttcgg
     9121 tcttcctcac tggcagttaa aatcgacctc tacagagagc ggcgtggtcg cgccggatga
     9181 acgattgccg tttgctcaga cggcagtaat gggcgttcaa cacgcggtgg cgatgtttgg
     9241 cgcgacggtg ttaatgccga ttctgatggg gctggatccc aatctttcca ttttaatgtc
     9301 agggatcggc acgttactgt ttttctttat taccggcggg cgcgtgccca gttatctcgg
     9361 ctccagcgcg gcttttgtcg gcgtggtgat cgccgcaacg ggctttaacg gtcagggcat
     9421 taacccgaat atcagcattg cgctgggcgg gattatcgcc tgtgggctgg tttataccgt
     9481 cattggcctg gtggtgatga aaatcggtac gcgctggatt gaacggctaa tgccgccggt
     9541 ggtgacgggt gcggtggtga tggcgattgg cctgaactta gcgcctatcg cggtaaaaag
     9601 tgtatcggct tcaggctttg atagctggat ggcggtaatg acggtgctgt gtattgggct
     9661 ggtggcggtg tttacccgtg gcatgatcca gcggttattg attcttgtag gattaattgt
     9721 cgcctgtctg ctgtatggcg tgatgaccaa cgttttaggg ctgggaaaag ccgtcgattt
     9781 tactctcgtc agccatgctg cctggttcgg tctgccacat ttttccactc ctgcttttaa
     9841 tggtcaggca atgatgctga ttgcgcccgt ggcggtgatt ctggtggcgg aaaacttagg
     9901 tcatctcaag gcggtcgccg ggatgaccgg acgcaatatg gacccgtata tggggcgagc
     9961 attcgtaggc gatggtctgg caacgatgct ttctggttct gtcggcagta gcggcgtcac
    10021 cacctatgct gaaaatatcg gtgtgatggc cgtgacaaaa gtctactcga cgctggtgtt
    10081 tgttgctgct gccgtcatcg ccatgctgtt gggcttttca ccgaaatttg gcgcgctgat
    10141 tcataccatt ccggccgcgg tgattggtgg tgcatcaatt gttgtgttcg ggctgattgc
    10201 cgtcgcaggc gcaaggatat gggtacaaaa ccgtgtcgat ttaagccaga acggtaattt
    10261 gattatggtc gcagtaacct tagtgctggg cgcgggtgat tttgctctca cgctgggcgg
    10321 ttttacgttg ggaggaatcg gtacagcaac ctttggcgcg attttactca atgcgttatt
    10381 aagcagaagg ttggttgacg ttccaccgcc agaagttgtt catcaggagc cataaacaga
    10441 aaaccgccgg ggttaccggc ggtgatggct ttttgacgag cttaagaaaa aaatgcattc
    10501 gtcattgatt agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta
    10561 cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc tcgatggttt
    10621 gccattactg acctccgtct gggtatagac atcatattgc tagaattaac gcggcgtatg
    10681 gcggctgcca tattttcgct acgtgggact aagcttagtg aactgacgtg tttctacccg
    10741 cgtttggtaa tatcctgcaa cagcttctta gcgagaatag atatcatctc caataattat
    10801 ccataagccg ctcttatgat taaaaaaacg cgatgagata aaaatgtgtc attttgcgac
    10861 aaaattgcgt gcttgtgaaa gttgttataa atcaaataag tggttgtgaa atttgcactc
    10921 tgaaaaggac gtcttatctt taaataagtg gtagcgaatc gctacggaat agagataaca
    10981 cgaggagtgg ttagaaatgg ctaaagttct ggtgctttat tattccatgt acggacatat
    11041 tgaaacgatg gcacgcgcag tcgctgaggg tgcaagcaaa gtggatggcg ctgaagttgt
    11101 cgttaagcgt gtaccggaaa ccatgtcgcc gcaattattt gaaaaagcag gcggtaaaac
    11161 gcaaactgca ccggttgcaa ccccgcaaga actggccaat tacgacgcca ttatttttgg
    11221 tacacctacc cgctttggca acatgtccgg tcaaatgcgt accttcctcg accagacggg
    11281 cggcctgtgg gcttccggcg cactatacgg aaaactggcg agcgtcttta gttccaccgg
    11341 tactggcggc ggtcaggaac aaaccatcac atccacctgg acgacccttg cgcatcacgg
    11401 catggtgatt gtccccattg gctacgcagc gcaggaatta tttgacgttt cacaggttcg
    11461 cggcggtacg ccgtacggcg caaccaccat cgcaggcggt gacggttcac gccagcccag
    11521 ccaggaagaa ctgtctattg ctcgttatca aggggaatat gtcgcaggtc tggcagttaa
    11581 acttaatggc taatcttcaa caggaggata cgcatgccaa ctcaagaagc gaaagctcat
    11641 cacgtcggtg aatgggcatc tctgcgcaat acgtcgccgg aaatagccga ggccattttc
    11701 gaagtcgccg ggtacgacga aaaaatggcg gaaaagattt gggaagaagg tagcgatgaa
    11761 gtgttagtca aagcctttgc taagaccgac aaagattcgc ttttttgggg cgaacagacc
    11821 atcgaacgta aaaacgttta accagcgact cccccgtagc gaacggggga gttttctgtt
    11881 atttcaccgc ttcattcaac acgctatcaa acttatccat cgggcagaaa ccatcagcgt
    11941 ctatcgggca accgcttaat tccagcgtca cgcgctgcgc aggtgcctgc agggttaacg
    12001 catcggcatt acgtaactgt tccgcactct ggtacacata ttcaattttc atcaaatcgc
    12061 gattggcttt gctgtcacgc caacgctgga aaacgatttt gccgccaatc ggcgtgcgtt
    12121 cgttctggtc atgcaactga tacggtttga aatccagcgc cgttaacaga gaggcaatgt
    12181 tggagtcatg cccaactaac accgtgattt ttggagcact ggtgcgatcg gtgaccaggg
    12241 ctttgtcgat ataactgacc agcggtttcg caacattgcg cgccacttcc ggtgaggtaa
    12301 acaggctgtc ctggtaaccg tttttcagct tcgacaacac cttccactgc tggtcagatt
    12361 tgatttctcc ccaggccacc tgatccatcg gaaaaccttc gtagtattgc aaagtaaacg
    12421 catctaccag cgagttgccg actttcagcg gcccggaaac acctggttct tgttgatact
    12481 tggcgctaaa ggtatttttg ccatccacca gcgaacactg ttgtttctct ttacaggcag
    12541 gggaatcttt atagttaacg attttttcca gtagctggta gctgtcggta agctggagtt
    12601 tgctgagctc tttctccatt gccgccaccg cctgttcact gaatgcggcg gaatcatcgg
    12661 tgatcaccgg gttaaaggtt gggtccatag tgcccatttt ttcctggtga tgcacaggaa
    12721 tatcacaccc cgggaatgcg ccggtaataa agaactgtgc ggtcgcaacg gtacgttgca
    12781 gactattggc ataggcataa acggtgtccg gcggcgggca ttcccccgat tttaccatcc
    12841 cctgctctgc cagccattca cgcatgtaat ggcccatata cacttcgagt acgccacctt
    12901 tggtggtgag ttgcccaccg gggacgtccc attcgggcca tttattcggc gtcgactgct
    12961 ccagcacact gccattgttc gccagcggcg cacgtaagtt atggcggctc atcatgagca
    13021 cttgctgtag ctgatagcct tccggtacgg tttgtgcctg agcgtttgaa gcgagtaaaa
    13081 ctacccctgc cacagctgcg gcgattagcg ttttgttcat tcctggcacc tcttttgtta
    13141 tcaatcacta aagagtgtga cagaaatatg acttttttcc gggaacaggt tcgcaaaatg
    13201 gcttgttgaa cggctgtttt tatattcatc tttaaaaaca ggcaaggttt gacgcaaact
    13261 atgcctgatt agcccctgcc tcgcgtacgc cgctgatgat tgcacctgtg gctaaacaga
    13321 atatcggcgg cgttaccaat acagaggcaa tatttaccaa taaaaaagtg tgagactaat
    13381 aacaatggtt tttagcatat gcctctgtca ttaaattgca taacgagtta aaaaaccaat
    13441 ttgcgcattc ctttccttat tgtttaactc gtaagcctga gcaaaaagtt caggagggag
    13501 gtaaacgtca taatgattgc cagtgtttat taaataacca tactcaagat cttcatctgt
    13561 tgaaaaggat tcattcccta aggagattga tgccacatta ctattaacaa taaataatga
    13621 gcaaatgcta tctgaaagag cagtgaggaa atttttcacc tcattaatcc tcttctctgg
    13681 gtcggtgtct aaaacaaggc gcgccttgtt atcattaaat ataaactcgg gtaaatgcag
    13741 tatgcatttt ttgtcattca agcaactatc aataaattct ttttttaaaa aacagaaagt
    13801 tccatgacat aagactaccc tgcccctgaa ttttgttgtc agattgtaga tcaacgccaa
    13861 ataacgtctt tacatcttcc ctgagattat agaatgccgt atcaacaaga gcctcgatcg
    13921 tttgtttatc aacaatttta ataaactctt cgtatacttg cttttgcgtc ttatggctgc
    13981 ccagcgccca tgaatactca tgttctttta aaaccagtat ggcccttaac agacaatagc
    14041 catcgccatc tactggagcg tattttaatc cataagtttt attaatttca ctctgaataa
    14101 aagatatacc agacaaatca ataccagaat ctaaatcaga tattcccggc gtatttatcc
    14161 attctggctg gatactcata ttttcatcat tatcaaaaca ataatcagaa taaacatcat
    14221 cacacgcatt aatgttttca atatattcca taaagccata ccaataataa cctttaaaat
    14281 ctgtgataga ctctgaacct ctacccggca aggatactct aacaatatca acatcttcaa
    14341 ca
//
LOCUS       DABSYT020000110        13994 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.110, whole genome shotgun sequence.
ACCESSION   DABSYT020000110 DABSYT020000000
VERSION     DABSYT020000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.110"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            39..2735
                     /gene="stcE"
                     /locus_tag="ID725_RS19805"
                     /old_locus_tag="pgaptmp_003961"
     CDS             39..2735
                     /gene="stcE"
                     /locus_tag="ID725_RS19805"
                     /old_locus_tag="pgaptmp_003961"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HGV7852450.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            2822..3697
                     /gene="gspC"
                     /locus_tag="ID725_RS19810"
                     /old_locus_tag="pgaptmp_003962"
     CDS             2822..3697
                     /gene="gspC"
                     /locus_tag="ID725_RS19810"
                     /old_locus_tag="pgaptmp_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HGV7852451.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3698..5665
                     /gene="etpD"
                     /locus_tag="ID725_RS19815"
                     /old_locus_tag="pgaptmp_003963"
     CDS             3698..5665
                     /gene="etpD"
                     /locus_tag="ID725_RS19815"
                     /old_locus_tag="pgaptmp_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HGV7852452.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            5665..7170
                     /gene="gspE"
                     /locus_tag="ID725_RS19820"
                     /old_locus_tag="pgaptmp_003964"
     CDS             5665..7170
                     /gene="gspE"
                     /locus_tag="ID725_RS19820"
                     /old_locus_tag="pgaptmp_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HGV7852453.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7172..8395
                     /gene="gspF"
                     /locus_tag="ID725_RS19825"
                     /old_locus_tag="pgaptmp_003965"
     CDS             7172..8395
                     /gene="gspF"
                     /locus_tag="ID725_RS19825"
                     /old_locus_tag="pgaptmp_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HGV7852454.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            8426..8860
                     /gene="gspG"
                     /locus_tag="ID725_RS19830"
                     /old_locus_tag="pgaptmp_003966"
     CDS             8426..8860
                     /gene="gspG"
                     /locus_tag="ID725_RS19830"
                     /old_locus_tag="pgaptmp_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HGV7852455.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            8857..9411
                     /gene="gspH"
                     /locus_tag="ID725_RS19835"
                     /old_locus_tag="pgaptmp_003967"
     CDS             8857..9411
                     /gene="gspH"
                     /locus_tag="ID725_RS19835"
                     /old_locus_tag="pgaptmp_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HGV7852456.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            9408..9773
                     /gene="gspI"
                     /locus_tag="ID725_RS19840"
                     /old_locus_tag="pgaptmp_003968"
     CDS             9408..9773
                     /gene="gspI"
                     /locus_tag="ID725_RS19840"
                     /old_locus_tag="pgaptmp_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HGV7852457.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            9770..10369
                     /gene="gspJ"
                     /locus_tag="ID725_RS19845"
                     /old_locus_tag="pgaptmp_003969"
     CDS             9770..10369
                     /gene="gspJ"
                     /locus_tag="ID725_RS19845"
                     /old_locus_tag="pgaptmp_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HGV7852458.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            10366..11343
                     /gene="gspK"
                     /locus_tag="ID725_RS19850"
                     /old_locus_tag="pgaptmp_003970"
     CDS             10366..11343
                     /gene="gspK"
                     /locus_tag="ID725_RS19850"
                     /old_locus_tag="pgaptmp_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HGV7852459.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            11277..12554
                     /gene="gspL"
                     /locus_tag="ID725_RS19855"
                     /old_locus_tag="pgaptmp_003971"
     CDS             11277..12554
                     /gene="gspL"
                     /locus_tag="ID725_RS19855"
                     /old_locus_tag="pgaptmp_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HGV7852460.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            12541..13053
                     /gene="gspM"
                     /locus_tag="ID725_RS19860"
                     /old_locus_tag="pgaptmp_003972"
     CDS             12541..13053
                     /gene="gspM"
                     /locus_tag="ID725_RS19860"
                     /old_locus_tag="pgaptmp_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HGV7852461.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            13174..13944
                     /locus_tag="ID725_RS19865"
                     /old_locus_tag="pgaptmp_003973"
     CDS             13174..13944
                     /locus_tag="ID725_RS19865"
                     /old_locus_tag="pgaptmp_003973"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGV7852462.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
BASE COUNT         3351 a         3101 c         4047 g         3495 t
ORIGIN      
        1 aaaaaataaa atatagaaat actgttatat ccggctgcat gaacactaaa atgaatgaga
       61 gatggagaac accgatgaaa ttaaagtatc tgtcatgtac gatccttgcc cctctggcga
      121 ttggggtatt ttctgcaaca gctgctgata ataattcagc catttatttc aatacctccc
      181 agcctataaa tgatctgcag ggttcgttgg ccgcagaggt gaaatttgca caaagccaga
      241 ttttacccgc ccatcctaaa gaaggggata gtcaaccaca tctgaccagc ctgcggaaaa
      301 gtctgctgct tgtccgtccg gtgaaagctg atgataaaac acctgttcag gtggaagccc
      361 gcgatgataa taataaaatt ctcggtacgt taacccttta tcctccttca tcactaccgg
      421 atacaatcta ccatctggat ggtgttccgg aaggtggtat cgatttcaca cctcataatg
      481 gaacgaaaaa gatcattaat acggtggctg aagtaaacaa actcagtgat gccagcggga
      541 gttctattca tagccatcta acaaataatg cactggtgga gatccatact gcaaatggtc
      601 gttgggtaag agacatttat ctgccgcagg gacccgacct tgaaggtaag atggttcgct
      661 ttgtttcgtc tgcaggctat agttcaacgg ttttttatgg tgatcgaaaa gtcacactct
      721 cggtgggtaa cactcttctg ttcaaatatg taaatggtca gtggttccgc tccggtgaac
      781 tggagaataa tcgaatcact tatgctcagc atatttggag tgctgaactg cctgcgcact
      841 ggatcgtgcc tggtttaaac ttggtgatta aacagggcaa tctgagcggt cgcctaaatg
      901 atatcaagat tggagcaccg ggtgagctgt tgttgcatac aattgatatc gggatgttga
      961 ccactccccg ggatcgcttt gattttgcca aagacaaaga agcacatagg gaatatttcc
     1021 agaccattcc tgtaagtcgt atgattgtta ataattatgc gcctctacac ctaaaggaag
     1081 ttatgttacc aaccggagag ttattgacag atatggatcc aggaaatggt gggtggcata
     1141 gtggtacaat gcgtcaaaga ataggtaaag aattggtttc gcatggcatt gataatgcta
     1201 actatggttt aaatagtacc gcaggcttag gggagaatag tcatccatat gtagttgcgc
     1261 aattagcggc acataatagc cgcggtaatt atgctaatgg catccaggtt catggtggct
     1321 ccggaggtgg gggaattgtt actttagatt ccacattggg gaatgagttc agtcatgaag
     1381 ttggtcataa ttatggtctt ggtcattatg tagatggttt caagggttct gtacatcgta
     1441 gtgcagaaaa taacaactca acttggggat gggatggtga taaaaaacgg tttattccta
     1501 acttttatcc gtctcaaaca aatgaaaaga gttgtctgaa taatcagtgt caagaaccgt
     1561 ttgatggaca caaatttggt tttgacgcca tggcgggagg cagccctttc tctgctgcaa
     1621 accgtttcac aatgtatact ccgaattcat cggctatcat ccagcgtttt tttgaaaata
     1681 aagctgtgtt cgatagccgt tcctccaccg gcttcagcaa gtggaatgca gatacgcagg
     1741 aaatggaacc gtatgaacac accattgacc gtgcggagca gattacggct tcagtcaatg
     1801 agctaagtga aagcaaaatg gctgagctga tggcagagta cgctgtcgtc aaagtgcata
     1861 tgtggaacgg taactggaca agaaacatct atatccctac agcctccgca gataatagag
     1921 gcagtatcct gaccatcaac catgaggccg gttataatag ttatctgttt ataaatggtg
     1981 acgaaaaggt cgtttcccag gggtataaaa agagctttgt ttccgatggt cagttctgga
     2041 aagaacgtga tgtggttgat actcgtgaag cgcgtaagcc agagcagttt ggtgttcctg
     2101 tgacgaccct ggtggggtat tacgatccgg aaggcacgct gtcaagctac atctatcctg
     2161 cgatgtatgg tgcctatggc ttcacttatt ccgatgatag tcagaatcta tccgataacg
     2221 actgccagct gcaggtggat acgaaagaag ggcagttgcg attcagactg gctaatcacc
     2281 gggctaacaa cactgtaatg aataagttcc atattaacgt gccaacagaa agtcagccca
     2341 cacaggccac attggtttgc aataacaaga tactggatac caaatcgctc acacctgcgc
     2401 cagaaggact tacctatact gtaaatgggc aggcacttcc agcaaaagaa aacgagggat
     2461 gcatcgtgtc cgtgaattca ggtaaacgtt actgtttgcc ggttggtcaa cggtcaggat
     2521 atagccttcc tgactggatt gttgggcagg aagtctatgt cgacagcggg gctaaagcga
     2581 aagtgctgct ttctgactgg gataacctgt cctataacag gattggtgag tttgtaggta
     2641 atgtgaaccc agctgatatg aaaaaagtta aagcctggaa cggacagtat ttggacttca
     2701 gtaaacctag gtcaatgagg gttgtatata aataatcctg agacggtgcg gccacttcgg
     2761 ccgcattagt aacacattcg tcccgttaat caaaacataa agtagcgtcc agcgttttat
     2821 tatgttgttc tttctatcat tccggggtga ccggggctta tttataaaag atatcgtact
     2881 taaaatgctg acgccaaacc ggctattgtg cgtaatttta cttattgccg gatatcagct
     2941 ggtgtcggtt atccatcatt tctggctgac tcaggcagca tcggtgcccg gcttatcccg
     3001 tgttagcgcg ccggaaacag cggtaactgg tgatcaaact gaagaacgtt ttgtttttac
     3061 gttattcggc agggcatccc cactatcatc ggaggggaga gcgcaggaaa caatgccttc
     3121 cctgtcagat gatctgcttt caggggagga tcttgacgtg agaggtatac tttatagttc
     3181 ggttgcagag cattccgttg ccatatttgc acataataac agacagttca gtctgagcgt
     3241 cggcgaaaaa gtacccagct acgatgctac gattagtgct atttttagcg atcatattgt
     3301 tattaactat cagggaaaaa ctgtatcact gcctctgcga tatgataata ccgaaaaaaa
     3361 gaatgcatat gacaataata atttaacagt tggagacgtg ataacccaag ataattttcg
     3421 ggtagaaagt gtttttgata ttatgagctt ttcagccgtt acggttaata atacattaag
     3481 cggttatcgc ttgattccgg gtaagcacag ttcgttattt tataatgctg ggttgcatga
     3541 taacgatctg gccgtatcgg ttaatggttc agaattgcgt gataccagac aggcgcagca
     3601 gataatgaag caattgccag aacttaaaga aataaaaata accgtcgagc gtgatggtca
     3661 gttatatgat gcatttattg ctgtaggaga aaactgattg ttcaggaaat ggttgaatgg
     3721 gcgtttgccg gtacttgtgt tcactacagt aattttgggg gccattccag ggtggggggc
     3781 tgaattttcg gccaacttta aagatacgga tattcaggag ttcataaata ctgtcagtaa
     3841 aaatttacac aaaacggtaa taattaatcc tgacgtgcag ggaaccatca ctgtacgcag
     3901 ctacgatatg ctgaacgagg aacaatatta tcagttcttt ctcagtgtgc tggacgttta
     3961 tggttttgct gtggtcgata tgcacaacgg tatactgaaa gtagtgcgct caaaagatgc
     4021 caaaacgtcg gcggtgccgg tagctagtga tgtcagtccc gggactggtg atgaggttgt
     4081 tacccgggtg gtccccgtaa gtaacgtggc agccagagat ctggcgcctt tgctgcgtca
     4141 gctcaatgat aatgctggcg caggaagcgt ggtgcattat gaaccttcta atgttttgtt
     4201 gatgaccgga cgtgctgcag tgatgaaacg gttgatggag attgttgaac gtgtggataa
     4261 ggtgggtaat cgcagcgttg ccacggtccc gctcacctac gcgtccgcaa cagacgtagc
     4321 cagacttgtt acggaactga ctaaagaaac agataagaca gctatacctg cttggatgac
     4381 ggcgaaactg gttgcagacg agaggacaaa ctcagtgctc gtcagcggag agccaatctc
     4441 ccaacagcgt atcatctcca taattaagca actggatcgt caggaggatg ttcagggtaa
     4501 tactaaggtg atttacctga aatatgcgaa ggcgaaggat ttagtggaag tcctgacagg
     4561 tatcagcagc agtattgaaa acgactctaa aaagagtccg tcaacggaag ccttgcgcaa
     4621 aggagtgacg attaaatccc acgaacaaac caatgccctg atcctgacgg gggcccctga
     4681 cgtcatccgc gaccttgaaa atgtgatttc gcagttggat attcgtcgtc ctcaggtcct
     4741 ggtggaggcc atcattgctg aaatacagga tgctgacggg ctgaaccttg ggatccagtg
     4801 ggtgaataaa catgccggtg tggcgcagtt caccagtacc ggtttaccta ttaccacgat
     4861 ggttcagact cgtcagaacg aaatcttaga cagcgatcag agcaatgccc tgagcatgtt
     4921 taacggaatt gcagcggggt tttatcaggg aaactgggcg atgctgttga cggcgctctc
     4981 cacaagtagc aagaatgata tcttggcgac ccccagtatt gtcacgctgg acaatatgga
     5041 ggccactttc aatgttggtc aggaggtccc ggtactttcg ggctcacaga caacctctgg
     5101 ggacaatatt tttaacacgg tcgagcgcaa aacggtgggg atcaaactca gggtaaaacc
     5161 ccagatcaac gagggtgatt ccgtgttact ggagatagaa caggaggtgt ccggtgtggc
     5221 ggacactgca gtagccacca ctactgactt gggagcaacc ttcaacaccc gaacagtgac
     5281 caatgccatg ctggtcggga atggcgaaac ggtggtggtc ggaggattac tggataagtc
     5341 gatcaggggg agtgagagta aagtgccact gctgggggat atcccggtac tggggcatct
     5401 ttttcgcgca aaaagcgaac agacagctaa gcgtaatctg atgctgttca ttcggccaac
     5461 tattattcgt gagcgcgacg gatttcgtca tgcttcggcc gaaaaatacc agtcgtttaa
     5521 tcaggaacag gtgcagtcgc gtggcaaaga aacaacggcg ctgacgctga atgaggaaca
     5581 gctcaggctg tcccccgatc aagacgatac ggctttccgg aaggtgaaag cggcgattgc
     5641 tgcgttttat gcgcaggaga tgtaatgagc agagtagtac agaatgtcag tgaatcacgt
     5701 ccccttctgc ctttttcctt ttcccgtacg cagagaattt tgttgctgcg tgaacaggag
     5761 gggaatcgag tgttctgcat ggaggacaca cctgccagtg ccctgttgga ggtgcgcagg
     5821 gtggctgaag gtcctctgaa tgtcacaaca gtgtcggcgg aagcgtttga aaaacagctg
     5881 gtgagcagct atcaacgtga ttctgatgaa gcgcgacaaa tgatggctga gattggtaat
     5941 gaaatggatt tttataccgt cgcaggggag ttgcctgacc gtgaggactt actggatgcg
     6001 aatgacgatg cgccaattat ccgcctgatt aacgcaatgc tgacagaggc aattaaagag
     6061 aaagcctcag atattcatat tgaaacttac gaacgccatc tgcaggttcg ctttcgcatt
     6121 gatggcgttc tgcgggagat cctccgttta cataggaatc tggcttcgtt actgatttca
     6181 cgcattaaag tcatggcccg gttggatatt gccgaaaaac gcgttcccca agatggccgc
     6241 atggtactgc gtatcggtgg tcgggcggtg gatgtgcgtg tttcaacgtt gccttcaaat
     6301 catggtgaac gcatcgtgct gcgtttacta gacaaaaata gcgttagtct cgatcttgct
     6361 gcactcggca tgtcgcagca gaatcagcga cacattgatg cactgatccg ccgtcctcac
     6421 ggcattattt tggtgaccgg gcctacaggg tcgggtaaaa gcacgacgct ttatgccgcg
     6481 ttaagcctgc tgaatccccg agaccgcaac attatgacgg tcgaggatcc tgttgaatat
     6541 gaactggacg gtatcagtca gacccaggtc aacccgaagg tggacatgac ctttgcccgg
     6601 agtctgcgcg ctattttacg tcaggatccg gacgtggtgc ttgtggggga gatccgtgac
     6661 ggtgaaacag cccagattgc tgtgcaggcc tcgctgacag ggcatttggt gctgtcaacg
     6721 ttacatacga atagtgcagc aggcgcgctg tcgcgtctgc aggatatggg catccctcct
     6781 tttctgcttt ccacctcatt actggctgtt ctggcccagc gactggttcg cacgttgtgt
     6841 ccgcgctgtc gtcagccctg tcaggtcagt actgagttag cgatggacat ggacattccc
     6901 cctgaaacca cgatctggca gcctgccggg tgtcagcact gcagtttcac gggctatcac
     6961 gggcgcaccg ggatccacga gctgttgctg attgacgatc gcattcggac ggcaatctat
     7021 cagggggagg gggagctggg tattacccgc ttggcaggga gtcgctatct gacgctgcgt
     7081 ggcgacgggc gacaaaaggt actagccggt gagaccagtt gggaggaggt ggttcgcgtt
     7141 actgaaagca gattgcagga agaggaatga tatggcgttg tttcattatc aggcatcaga
     7201 tattcacggt agaaaacgca gtggcattct ggaggcggat tcggcccgac atgcccgtca
     7261 gttgctgcgt gagcaggcgc ttattccagt caggttggat gagaaacagg tccatcacaa
     7321 gcactcactg cggagtatcc tgaggtttcg tccgcgcggg gggagcagcg ccgaactcgc
     7381 gttactgaca cggcagttag ccacactagt ggccgcatct ctgccgcttg aagaggcgct
     7441 ggatgcgttg ttgaggcaga gtgaaaaacc gcgtcagcgg aacttaatcg ctgccgtgcg
     7501 caccaaggtt ctcgagggac attccttggc cgcggcgatg ggcatgtttc caggtacgtt
     7561 tgagcgtctt tattgtgcga tggtggcggc gggggaaaca tccggtcgtt tggacgtggt
     7621 gctcagtcgt ctggctgact ataccgagca gcgccagatc atgcgaaacc gtctactcca
     7681 ggcattgctc tatccttgtg ttctgacgtt ggtggcggtt ggggttattg ccattctgct
     7741 tactgcggtt gtgccgaaag tggttgagca gtttattcat atgaaacaga cccttccctt
     7801 atctacccgc gtactgatgg gggctgctga ggtgagccag acttggggcc cgtggctgtt
     7861 actggccgca gcgctgggcg ggatagcggg gcgcatgata ttacatcagc cctcccagcg
     7921 tctggctttt catcatctgc tgttgcggct gccggtcgtg gggcgcatat cgcgcggtct
     7981 gaataccgca cgttatgccc gcacgctgag cattcttaat gccagtgcgg taccgctgct
     8041 ccaggcgatg cacatcagtg gtgacgtact gagtaatgac tgggcccgtc atcagttagc
     8101 taccgcggcc gagttggtta gggaaggggt cagcctgcat caggcactgg agcagacttc
     8161 gctgtttccg cctatgatgc ggcacatgat tgccagcggt gaaaatagtg gcgaactcga
     8221 cagcatgttg gaacgggccg ccgacaatca ggatcgtgag ttcagcacac agatgcaact
     8281 ggcgctggga ttgtttgaac cgctgttggt ggtgggtatg gccggggtcg ttttgtttat
     8341 tgttctggca attctgcagc cgctcctgca gctcaacaac atgatgaata tgtgaatcct
     8401 tctttaacag tcaacggaga aaaacatgcg aaaacagcat cagcgtggtt ttaccttgct
     8461 ggaaataatg gtggtaattg tgatcttggg agttttggcc agtctggtgg tcccgaatct
     8521 gatgggaaat aaggataagg ccgatcggca gaaagtgatg agcgacttgg ttgcgctgga
     8581 aagcacgctc gatatgtacc ggttagataa taaccggtat ccgacaacgg agcagggact
     8641 gcgggcactg gtcagtaaac ccaccgtgca acctgaaccc cgtaactacc ggcaggatgg
     8701 ttatatccgc cgtttgcccc aggatccttg ggggggggat tatcagctgt taaatcctgg
     8761 tcagtacagc gacattgata tcttttctcc tggcccggat ggtgtgccaa acactgaaga
     8821 tgatattggt aactggacgc tcggtaacgc gcagccatga gtcagcgcgg tttcacaata
     8881 ctggagatga tgctgatcct tttgttgatg ggcatcactg cagggctggt actaatgtca
     8941 tttcctgact ctgctcagaa tcatctgcaa caacagcggg agcgtctgca ggcgcagctg
     9001 gattatgcct tggatagaag ccagcaggac ggcttgctga tggggattca ggttcggttt
     9061 gacggatgga aatttaaggt tctgcagcgc ggaacagcgg aaagcccccc gacattagct
     9121 gagggagggg atatttggca ggggtatgtc tggcaaactt ggcaaccgcg acgtgcagcc
     9181 atggggggca aacttccgga tgatgtgcgt cttgagctgc agtatctcag aggtttgcag
     9241 tggatgtctg agcatgatga cggggctgaa cccgatattt tgctgcttcc cggaggtgag
     9301 gtaacgccgt tcagacttct tttcaggcag gtcggagagg aggccgttgt cgggctgcag
     9361 gttgatgaga acggtttgat gactcttttt gaaggtgagg tctcattgtg aaggggcaag
     9421 aaggaatgac attactggaa gtcatagtag ctctggttgt gtttgcgctg gcaggaatgg
     9481 cgctgatgca ggcctcgacc cagcaggctg cggggatcgg acggatggaa gagaaggtgc
     9541 tggcaggctg gttggccgat aaccagatgg tgcaattgca actcgagaaa acttggccgg
     9601 aaaatggctg gggcgagaag acgatcagct tcgctggcac cgaatggtat ttacgctggg
     9661 gcgggccgga cagcgacgtg ccgcagccgc gctccttaga ggtagaggta cggcgcacta
     9721 aagaggagac gtccgccttg gtttccttgc gtagcagtgt ggttcgtgaa tgagtcagca
     9781 acgggtaaaa ggttttacgc tactggaaat gctgttggca ctggctgttt ttgcagcgtt
     9841 gagcatcagc gcatttcagg tgctgcaggg tggaatacgg gcgcacgagc tgtcccggga
     9901 taaagtacag cgtctggctg agctgcagag agggatcagc cagatggagc gagatcttac
     9961 gcagatgctc ccaaggcaca gccgggggaa tgaaggcctg ctgctcgctg cacctcatct
    10021 gctaaaaagt gatgactggg ggatttcttt tacgcgaaac agctggctga atcccgccgg
    10081 gatgctgccg cgcccggagt tacagtgggt aggttatcgg ttgcgacagc aaaagttaga
    10141 acgcctgagc tactttcatg tcgatcaccc ttccggtgtt tctccggatg tcagggtgat
    10201 gctggacggc gttcacgcat ttcgtctgcg tttttttgtt aacggtgatt ggcaggcgcg
    10261 ctgggacagt accggcatcc tgccgcaggc tgttgaagtg acgctggtta tggatgattt
    10321 tgctgaactg ccccgtctgt ttcttgtgag caaggagacc gctgaatgaa attgcgggaa
    10381 cagggcgtgg cgcttctggt ggtattgctg atcctgtcgt taatggtaac gatagcggcg
    10441 gtgattgcgg agcgcaacgg tcggacattt ttgcgtacag tagcgcagtt ggatcagctg
    10501 caggcaaagt gggatggcta tacggcagag acgatagcaa aacaaatctt acagaggagc
    10561 aggcaggagt cgccccggaa aacgcatctg gcacaaaatt gggcgcagtc agaacgacag
    10621 tttgagacca gagggggaga tgtgcggggg cagatcgtgg atgcgcaggc ctgttttaat
    10681 cttaatgcta ttaattatgg agtggtggat ctgaccagta taccttatgc cgccagaatt
    10741 tttcagcagc tattaatcaa cttgcaggta gagcttttac aggcccggca agtgacggca
    10801 gccctacgtg actggatcga tcgtgatgat aagccggtaa ggggcggtgc tgaagacgag
    10861 gtgtatatgg ggatggaacc tccctatctg gcagccaacc agccgatgca ggacgtcagt
    10921 gagttacgcc ttatccgggg aatagatgcc aggctttacc gcaaactttt gccgtatgtt
    10981 tgtgtactgc ctacgtcaga tctgtctgtg aatgtgaata ctctgctgga ttcacaggcg
    11041 ccgctgctgg cggcgctctt tctgacgaaa ccggacagtc ttccggtgac ggaattactg
    11101 cagcggcgac cccgtactgg ttgggagagc gtggcggcgt ttcttgacgc gccaccgctt
    11161 aaggacattg acaccagtgc ggccatgccg gtattggccg tcagcagcaa ctattttctg
    11221 gttcggttac atgtgcgctc cggggaacac ctgttcagtc agcagacttt aatgcagtgg
    11281 agggaggagc gtttccggat cattcagcga cagtatggat taacgatgag ggaggttccc
    11341 tgaaaaaaaa taacaaacat gtcgaggctg gtcggctttt tctgcgactg ccggaggatg
    11401 actcggagcc tttattgtgg aggctgattg cgccagagga gacaggagcc ggcagtgtgg
    11461 cagtcggaga aacggatccg cagttagtcg gtttgctgtc ccgttatccc gcctgggtgc
    11521 tggttccgtc aggcagaatt gccttccatc aggtcacgct gccacaccgc acgtggcgtc
    11581 agcgtttgca ggtgctgccc tttcttctgg aagagcagtt ggcgacagac attgagcagc
    11641 tgcacttcgc cattcttcat cagtcgggtg acaggtgtga tgtggcggtg gtcgaaaaag
    11701 cggttatgca tcgctggtta gcgtactgtg gacaacttgg ggttcgggag gtgaggttgc
    11761 tgcctgacgt actgatgctg ccgttggcaa ctgaaggatg gagtgccgta aagcttgccg
    11821 ggcagtggct gtttcgccgg gatcgttacg ccgggatgac ggtagagtta tcgtggctga
    11881 cacatttctt gtctcttact gcgccgccgg tgattgagtc ctactccgtg ccgccagtac
    11941 cgtgcccggg tccgaagacc actgagtggc gaaatcagcc cggacgcgat ctgctgcagt
    12001 tggtggccga aggtgacggg tatgatggcg cagatctgcg gcagggagaa tttgcccgcc
    12061 agggaacatg gtatgcacga tttcgcccat ggaggtatgt cgcaggggct ctgctggcct
    12121 gtgtttttct ggccgggagc aacgcggggc tggcacatta tcggctctgg cagcaggctc
    12181 aattctggcg gtatgagagc gtacgtgtct atcagcagct gtttccgagt gaaaaggcgg
    12241 tgaaggatcc ccgccggcag atgctgagac atctgcaaca gtcgacgggt aacaatatac
    12301 cggaactcgg gagcgtgatg cgccagcttc agcaactgct gtcagaaacc tcaacgatac
    12361 gcttacaggc actcgcctgg gacagcagca gccagacact gaaaattgat ttgcaggcgg
    12421 cttcttttca ggcgctggaa cactttcagc agatcgccgg agaaaaatac ctcatccagc
    12481 ctggtgagat acgacagcag ccgcaggggg tagaaagtcg tctgatcctg agagtgaaca
    12541 atgaacgagc ttaaaaaacg tttgcagtat gttagccccc gtgagcgcag gctgcttatc
    12601 ggatgtgttg ccttactggt tgttgttttt gtctattacg tcctctggca gccctggctg
    12661 atacgagagg ataagtggcg gactgtagtt acccgtgaaa aaaacacagt ggagtggatg
    12721 aaacaacagg tacccaacat aaagagacgg gaggcaagta tgcctgaaca gggggagccg
    12781 ttaggtttat ctgcggccgt gacacgtact tcagccgcat acggtgtatc ggtcacccgg
    12841 ttgcagcctc aaggggagcg tctggccgtc accttagccc cggttgaatt taatgcgctg
    12901 atgcagtggc ttacgcaact ggagcgtcgg caccgtattc gtaccatggt atttgatgtg
    12961 gccgcgcagg gcaacccacc cggacaggta acggttaatc ggctggtttt gtcccaagat
    13021 gacgtaaaca aggacgcttc gcttagtcgc tgatgtcgta atttacagat taatgcttga
    13081 tgtaaaaatt ggacttataa aggaggatgt gtgagtagtt tgccggaatt tgcggttgat
    13141 tatcccttgt tgtggctcat ctgcaccgga gggctggggg gcgttaccgg cagttttctt
    13201 aacgtcgtga ttcatcgtct gccgataatg ttggagaggc ggtggcggcg tgaagcgctg
    13261 acgctattgg cacattctgt gcctgagtcc gggccggcct ttaacctgat gataccgcag
    13321 tctcattgtc cctgctgttt tcatccgctc ggcatcaggg ataacattcc cctgttaagc
    13381 tacctatttc tgaaggggaa atcacgctgt tgtggcgagc gtattcccgt ttactacccg
    13441 ctagtcgaaa taacgaactc agtgttattc attctggccg cgagcaggtt tccaccaggg
    13501 ctgacattag cggctgcctg gctttttatt tccatgctgt tggttctggc ggttattgac
    13561 tgccatacgg cgctgctgcc ggatgtgctg acgttgccat tgctctggct tggcctgtta
    13621 ttcagtttac agagaggcgt tgtcacctta gaggaggcgg tagtgggggc ggtatctggg
    13681 tatctctgtc tgtggggact gtactggctt tttcgtttcg ctaccgggag ggaagggtta
    13741 ggttacggtg attttaagct agctgcggca ttgggagcat ggatgggttg gcaggcatta
    13801 ccctcaatac ttttgtttgc ctcagtcagt gggttaatcg taacagtact gttgcgtatt
    13861 gtaacagcta cggactttac ccgcccgctg ccttttgggc cgtggctcgc tttgagcgga
    13921 gtgtgccatt ttttgttgat gtagcgttcg gttaccgggg ctagtaataa aaacggctca
    13981 gcggataaaa gaac
//
LOCUS       DABSYT020000111        13817 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.111, whole genome shotgun sequence.
ACCESSION   DABSYT020000111 DABSYT020000000
VERSION     DABSYT020000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.111"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(114..938)
                     /gene="dinD"
                     /locus_tag="ID725_RS19870"
                     /old_locus_tag="pgaptmp_003974"
     CDS             complement(114..938)
                     /gene="dinD"
                     /locus_tag="ID725_RS19870"
                     /old_locus_tag="pgaptmp_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGV7852463.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(1159..2022)
                     /locus_tag="ID725_RS19875"
                     /old_locus_tag="pgaptmp_003975"
     CDS             complement(1159..2022)
                     /locus_tag="ID725_RS19875"
                     /old_locus_tag="pgaptmp_003975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGV7852464.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            2149..2865
                     /gene="rph"
                     /locus_tag="ID725_RS19880"
                     /old_locus_tag="pgaptmp_003976"
     CDS             2149..2865
                     /gene="rph"
                     /locus_tag="ID725_RS19880"
                     /old_locus_tag="pgaptmp_003976"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGV7852465.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            2931..3572
                     /gene="pyrE"
                     /locus_tag="ID725_RS19885"
                     /old_locus_tag="pgaptmp_003977"
     CDS             2931..3572
                     /gene="pyrE"
                     /locus_tag="ID725_RS19885"
                     /old_locus_tag="pgaptmp_003977"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGV7852466.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3609..4205)
                     /gene="slmA"
                     /locus_tag="ID725_RS19890"
                     /old_locus_tag="pgaptmp_003978"
     CDS             complement(3609..4205)
                     /gene="slmA"
                     /locus_tag="ID725_RS19890"
                     /old_locus_tag="pgaptmp_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGV7852467.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(4312..4770)
                     /gene="dut"
                     /locus_tag="ID725_RS19895"
                     /old_locus_tag="pgaptmp_003979"
     CDS             complement(4312..4770)
                     /gene="dut"
                     /locus_tag="ID725_RS19895"
                     /old_locus_tag="pgaptmp_003979"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGV7852468.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(4748..5968)
                     /gene="coaBC"
                     /locus_tag="ID725_RS19900"
                     /old_locus_tag="pgaptmp_003980"
     CDS             complement(4748..5968)
                     /gene="coaBC"
                     /locus_tag="ID725_RS19900"
                     /old_locus_tag="pgaptmp_003980"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGV7852469.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            6140..6808
                     /gene="radC"
                     /locus_tag="ID725_RS19905"
                     /old_locus_tag="pgaptmp_003981"
     CDS             6140..6808
                     /gene="radC"
                     /locus_tag="ID725_RS19905"
                     /old_locus_tag="pgaptmp_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGV7852470.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            7025..7261
                     /gene="rpmB"
                     /locus_tag="ID725_RS19910"
                     /old_locus_tag="pgaptmp_003982"
     CDS             7025..7261
                     /gene="rpmB"
                     /locus_tag="ID725_RS19910"
                     /old_locus_tag="pgaptmp_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGV7852471.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            7282..7449
                     /gene="rpmG"
                     /locus_tag="ID725_RS19915"
                     /old_locus_tag="pgaptmp_003983"
     CDS             7282..7449
                     /gene="rpmG"
                     /locus_tag="ID725_RS19915"
                     /old_locus_tag="pgaptmp_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGV7852472.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            7547..8356
                     /gene="mutM"
                     /locus_tag="ID725_RS19920"
                     /old_locus_tag="pgaptmp_003984"
     CDS             7547..8356
                     /gene="mutM"
                     /locus_tag="ID725_RS19920"
                     /old_locus_tag="pgaptmp_003984"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGV7852473.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(8395..8874)
                     /gene="coaD"
                     /locus_tag="ID725_RS19925"
                     /old_locus_tag="pgaptmp_003985"
     CDS             complement(8395..8874)
                     /gene="coaD"
                     /locus_tag="ID725_RS19925"
                     /old_locus_tag="pgaptmp_003985"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGV7852474.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(8882..10159)
                     /gene="waaA"
                     /locus_tag="ID725_RS19930"
                     /old_locus_tag="pgaptmp_003986"
     CDS             complement(8882..10159)
                     /gene="waaA"
                     /locus_tag="ID725_RS19930"
                     /old_locus_tag="pgaptmp_003986"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGV7852475.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            10572..11630
                     /gene="rfaQ"
                     /locus_tag="ID725_RS19935"
                     /old_locus_tag="pgaptmp_003987"
     CDS             10572..11630
                     /gene="rfaQ"
                     /locus_tag="ID725_RS19935"
                     /old_locus_tag="pgaptmp_003987"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGV7852476.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            11627..12751
                     /locus_tag="ID725_RS19940"
                     /old_locus_tag="pgaptmp_003988"
     CDS             11627..12751
                     /locus_tag="ID725_RS19940"
                     /old_locus_tag="pgaptmp_003988"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGV7852477.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            12744..13541
                     /gene="rfaP"
                     /locus_tag="ID725_RS19945"
                     /old_locus_tag="pgaptmp_003989"
     CDS             12744..13541
                     /gene="rfaP"
                     /locus_tag="ID725_RS19945"
                     /old_locus_tag="pgaptmp_003989"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGV7852478.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            13584..>13817
                     /locus_tag="ID725_RS19950"
                     /old_locus_tag="pgaptmp_003990"
     CDS             13584..>13817
                     /locus_tag="ID725_RS19950"
                     /old_locus_tag="pgaptmp_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGV7852479.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLA"
BASE COUNT         3414 a         3333 c         3581 g         3489 t
ORIGIN      
        1 aaaattgagg ggtggaaaag aaaggtgcaa tgtttagcca ttgtgttcac cgcacctgga
       61 ctttggctta cgcatcgggt atctcaatca acaaaaagag tttttagttt ctgctatttc
      121 ttctctgtaa tttttacact gttttccaac tgcttgatgc tgacctgcgg ggtcggcaac
      181 tcctcaggca tggttccgcc aagttcctga atggtttgcc tcaccttgct acccacatca
      241 aagtgtgtgg tattagcttg ctgttttgaa ttcacgccat cccgcttgag tttttcttct
      301 gtttgggtag ctcgaaagag attagccgcc agttctgttg aacccatatg atccaggatc
      361 ttctgactct ttttcagccc cttcagttgg tggatggctt tctgatctaa tccaccatac
      421 agcccctggt aaccatgatt ctgaaatatg gcgaaatcag tagctgtcgc taccgcagct
      481 tgctgtgctg cctcaaccaa ttgtttgtta tgttctttca actcatttct cagaaacaga
      541 cgcttttcgt cttcacgaag ttgcttaaat gcttcatcgt cagcaagctc ctgccgtcgg
      601 gtctggatag caaaataagt ctgccctgcc gcaatgaccg gtttcgcagg gtcgccgttt
      661 tgtactacca ggtaacaggc atagcgggag agatgaacat cttcaagttc tcgttgagcc
      721 cctgaaccta atacaaccat tttggtggtt tcaacgaaat ggtcagatgc agcttgattg
      781 ctggcttcgc acgcttgagt cgcgcgggcc agtacctttt gaaaattgcg ccagtcccga
      841 taatccagta acggtgccaa ttctctagct gaccaaaatt cttgcccttc agtgccataa
      901 tgccgtattt cttcaaaagg ttgatgatgt tcgttcattg cccactccat gtgatatcga
      961 ttgcaaagaa agtaaatctg taactgtatt tatatacagt tgtgagtatc tcacgttctc
     1021 agtgtgctgg gcaaaaaaac atcacaaaat ctggagcagg cacgcaaaac agaattgaag
     1081 ttgcggggaa gaaaaatgcc gctctgtttg agtgggcggc atttgtgaag gctgaaaagt
     1141 aagaattggc gttacgagtt attcaatgtt ctgaatctgc tcgcgcatct gctcaatcaa
     1201 cactttcagc tcaatcgcgg agtttgtcac ttcggcattg atagacttcg acgcaagagt
     1261 gttcgactcg cggttgaatt cctgcatcat aaagtccaga cgacgaccaa cggcttcttt
     1321 tttcttcaga atgttgtagg tctctttgac atgcgcttcc aggcggtcca gttcttcggc
     1381 aacgtcaatt cgttgtgcca gcagaaccag ctcctgctcc agacggttgt tttccaactg
     1441 aacctgagca tcttccagct tcgcgaccag acgctcacgc tgccattgca ggatttccgg
     1501 catatgggcg cggactttga ccacttcggc ggtgacgcct tccagacgct gctcgatcaa
     1561 tgctttcagt gcctgacctt cggtttcgcg cgcgacaata aagtcgtcca gcgtaccatc
     1621 cagcgccgcg agaatttcag cggcaatggc gtcaagatcc tgctcctggg ctgccatcac
     1681 gcccggccag cgtagaatat caaccgggtt gatttcccct tcgtcactct gcatttttac
     1741 ccagttcgcg gcagttacca gctgtttagc cagtttttcg ttgaggatca gctcaccttg
     1801 cgcgctaaca tctggctcat agcgcagggt acattccact ttaccgcgcg tcaggcgaga
     1861 acgaatacgc tcgcgaacga caggttcaag gctacggaac tgctccggca gacgaaagta
     1921 agtttccaga taacgctggt ttaccgagcg catttcccag gttgcgctcc cccattcacc
     1981 cttgatttca cgccgggcgt aggcggtcat actgcggatc atagacgttc ctgtttataa
     2041 aaggagaggt ggaaggatta tagccatcga tgccttgtca ggataggaat aaccgccgga
     2101 agtccgtata atgcgcagcc acatttgttt caagccggag atttcaatat gcgtccagca
     2161 ggccgtagca ataatcaggt gcgtcccgtt accctgactc gtaactatac aaaacatgca
     2221 gaaggctcgg tgctggtcga atttggcgat accaaagtgt tgtgtaccgc ctctattgaa
     2281 gaaggcgtgc cgcgcttcct gaaaggtcag ggccagggct ggatcaccgc agagtacggc
     2341 atgctgccac gttctaccca cacccgtaac gctcgtgaag cggcgaaagg taagcagggt
     2401 ggacgcacaa tggaaatcca gcgtctgatc gcccgtgctc ttcgcgcggc agtagatttg
     2461 aaagcgctgg gtgagttcac cattacgctg gactgcgacg tgcttcaggc tgatggtggc
     2521 acgcgtaccg cgtcgattac gggtgcctgc gtggcgctgg cagatgcgct acagaagctg
     2581 gtggaaaacg gcaagctgaa aaccaacccg atgaaaggga tggtagccgc agtttctgtc
     2641 ggaattgtga acggcgaagc ggtttgcgat ctggaatacg ttgaagactc tgccgcagag
     2701 accgacatga acgtagtgat gaccgaagac gggcgcatca ttgaagtgca ggggacggca
     2761 gaaggcgagc cgttcaccca tgaagagcta ctcaccttgt tggctctggc ccgaggggga
     2821 atcgaatcca ttgtagcgac gcagaaggcg gcgctggcaa actgattttt aaggcgactg
     2881 atgagtcgcc ttttttttgt ctgtagaaaa gtaagatgag gagcgaaggc atgaaaccat
     2941 atcagcgcca gtttattgaa tttgcgcttg gcaagcaggt gttaaagttt ggcgagttta
     3001 cgttgaagtc cgggcgcaaa agcccctatt tcttcaacgc cgggctgttt aataccgggc
     3061 gcgatctggc actgttaggc cgtttttacg ctgaagcgtt ggtggattcc ggccttgagt
     3121 tcgatctgct gtttggccct gcttacaaag ggatcccgat tgccaccact accgccgtgg
     3181 cgctggcgga gcatcatgac cttgacctgc cgtactgctt taaccgcaaa gaggcaaaag
     3241 accacggtga aggcggcaat ctggttggta gcgcgttaca aggacgcgta atgctggtag
     3301 atgatgtgat caccgccgga acggcgattc gcgaatcgat ggagattatt caggctaatg
     3361 gcgcgacgct tgctggcgtg ttgatttcgc ttgatcgtca ggaacggggg cgcggcgaga
     3421 tttcggcgat tcaggaagtt gagcgtgatt acaactgcaa agtgatctct atcatcaccc
     3481 tgaaagacct gattgcttac ctggaagaga agccggaaat ggcggaacat ctggcggcgg
     3541 ttaaggccta tcgcgaagag tttggcgttt aaagaaactc gccggatgaa aagtcatccg
     3601 gcgtcatatt actgcaactg tgccgcaatt agcggccagc gggcgtcaaa atcatccgtc
     3661 gggcggtatt taaattcgct gcggacaaaa cgtgacagca taccttcaca gaaggctagg
     3721 atctggcttg ccagcagggt ttcatcggtg gtgtaacctt caccctcacg cattctcttt
     3781 tcacgcaata cctgacgcag ctgcgcttca atacgctcga acagctggtt gatgcgccct
     3841 tgcaggcgat cctgttcaaa cattagcgca tgaccagtga ggatgcgggt caggccagga
     3901 ttacgctcac caaaaccgag aagcagcaac acaatcagac gcaggcgcgc tgtggtgtct
     3961 ttctcatctt tcagaatcag attgatgcga gtaatcaggc tatcttcgat aaactcaatc
     4021 aggctatcga acatgcgggt cttactgggg aagtggcgat acagtgccgc ttcggaaacg
     4081 ccgacagagg cggccagttt tgccgtcgtg atacgttggc ttccatcgct ggattccagc
     4141 atcagcgcca gagactgaag tatttcctcg cgacggttcc ttttcgcagt ttgtttttct
     4201 gccatgttac aaaatacccc tgaaaataag cacttgccag gcggcaccca cgctatgacc
     4261 gcaaacgaaa tgtttacggc tatgttatga cgttattcgg atgcgtatgt gttactgacg
     4321 accagagtga ccaaagccgc cttcaccgcg gtcggtggcg tcgaaatctt ccaccagatt
     4381 aaattcagcc tgtactaccg gaacaaaaat catctgggcg atacgttcgc caggttgaat
     4441 ggtgaagctg tcctgaccac ggttccacac ggaaatcatc aactggccct gatagtcgga
     4501 atcgatcaat cctaccaggt taccaagcac gataccgtgc ttatgtccca atccggagcg
     4561 cggcagcatc attgccgcca gtgaaggatc ggcaatatga atcgccagcc cggtcggaac
     4621 cagcgtagtg tcacccggag ccagttctac ggcgtcgtcg agacaggcac gcaggtcaag
     4681 tccggcagag ccagaggtgg cataagtcgg gagcggaaat tccttcccaa cgcgcgggtc
     4741 cagaatctta acgtcgattt ttttcatcat aacgggtcac gatctcgtcg agtaataatt
     4801 ggccaaggag ctctttgcgc tcaagcggta agactttatc tccgtcctgc cagaaaaggt
     4861 gtaatgcgtt gttgtcgctg ttaaatcctt gagttggctg ggaaacatcg ttcgcgcaga
     4921 tcagatcaag gtttttacgg atacgttttt gccgggcgta ttcttccaca ttatttgttt
     4981 cggcggcaaa tccaacgacg tagggtcgat ggtcttttag tgcggcaacg cctgcgacga
     5041 tatcggggtt tttaaccatt tttattgtta attcatcacc ctgcgtggcc tgctttttga
     5101 ttttctctgg ggccacggta gctgcgcgat aatccgccac ggcggcgcag ccgataaaaa
     5161 tattttgctg ctgtacagaa gcattcacgg cggcttccat ttccagcgcg gtcatcacat
     5221 caacacgttt aacaaacggt ggcgtcggta gtgaaaccgg acctgatacc agcgtgacgt
     5281 tcgcgccacg acgggcagcg gcggcggcga tagcaaaacc catcttgccg gagctgtgat
     5341 tagagatata acgcacggga tcgagcggtt cacgcgtcgg gccggcggta atcataatgt
     5401 tcagatgttt caggtcgttg acgggcgaaa aatgcgctac cgccatatcc acaatggtta
     5461 acggatcgag cattcgccca ggaccgatat caccacaagc ctgactgcca ctgtctggcc
     5521 cccagatgag caaaccacgg gaagcaagca cctctaaatt atgctgcgtg gcagcggcac
     5581 ggtacatctg ctggttcatg gcggggagca cggctacagg cgcaggtgta gccagacaaa
     5641 tcgtcgatac caggtcattc gccattccgg cagcaacacg ggcaatcaaa tctgccgtgg
     5701 caggggcgag aatcactaaa tcagcccatt tacccagctc aatatggccc atagcggctt
     5761 cggctgccgg gtccagcaaa ctgtcggaaa cgggataacc agaaaccgcc tgcaagctaa
     5821 gtggggtgat aaaggctttt gccgcttcgg tcatggctac gcggacgtcg gccccgcgat
     5881 cgcgcaaacg acgcaccagt tcaggggttt tataggcagc aataccgccg ctaacgccga
     5941 gaacgatttt tttaccggcc aggctcatga tgatttttcc tgttgggtga caccagaagt
     6001 tggcgatttt atcacaatac ttttgttgtc gtgccttcac ctgagattca ctttgcgagg
     6061 cgctttccag gattgaaaac tggccgtcga tttaacggaa cggctatgac aggatgcgag
     6121 caccacaaag gaggtgaagg tgaaaaacaa tgcacagctg ttgatgccgc gcgaaaaaat
     6181 gctgaagttt ggtattagcg ccttaactga tgtcgagctg ctggcgctat ttctgcgtac
     6241 cggaacgcgc ggtaaagatg tattaaccct ggcaaaagag atgctggaga atttcggctc
     6301 tctttatggc ttgttaacct ctgaatatga acaatttagt ggtgttcatg gaattggcgt
     6361 agcgaaattt gcccagttaa aggggattgc tgaactggcg cgacgttact acaacgtgcg
     6421 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
     6481 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactccc aacaccgggt
     6541 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
     6601 aattatccgc gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
     6661 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
     6721 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
     6781 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
     6841 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
     6901 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
     6961 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
     7021 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
     7081 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
     7141 ggttgagagc gagaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
     7201 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     7261 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     7321 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     7381 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     7441 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     7501 ctgcttgccc ccatattgac tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     7561 cgaagttgaa accagccgcc gcggcataga accgcatctc gttggtgcaa ccattcttca
     7621 tgcagtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     7681 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     7741 gggctggatt atcattcatt tagggatgtc tggcagcctg cgcatccttc cagaagaact
     7801 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     7861 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcataatgt
     7921 gctggcccat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     7981 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     8041 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     8101 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     8161 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     8221 taaaccgggc tatttcgctc aggaattgca ggtttacggg cgaaaaggtg agccgtgtcg
     8281 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     8341 gcagtgccag aagtaattca tgcgcgccgg atggcatacc atccggcata aacgctacgc
     8401 taacttcgcc atcagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     8461 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     8521 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     8581 atattcaaaa tctgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     8641 ggcgaagttc gccatcaaat cactaaaccc gaccacttcc acgttcccca gatgcgcggt
     8701 tgcctgctgt gccagtgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     8761 ggcggcaatc gccagaataa cgtgatcgaa catctgcgtg gcgcgcgtca cgatatcgat
     8821 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     8881 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     8941 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     9001 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     9061 cgcttgcctg ctccagccgc gcgcaaatgt ctttaaagtt aaaagtatgc ggccccatca
     9121 ataccggaat tgcgtgtgcg gcagcttcca gcggattatg cccaccacgt tcaaccagtg
     9181 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     9241 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     9301 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     9361 ccaggatgag caataaattc gggaattgct gtaataaagc ctgatgtgcg gcaatgacca
     9421 cgctctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     9481 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     9541 ggctaccggt aacggttacc tggttatttt tcgcgcccag cgccacaaaa cgtgcaccat
     9601 cttcttcatt ttgggcggca atcagcgtaa tacgacgcag caagcgacgg acgaatttac
     9661 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     9721 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     9781 acaccagttt agggtcgact ttattcagga aacggttgag tgcatcgggc agatcatacg
     9841 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     9901 gcgtcatggt tgttacggta ataggtaaat caggataacg atgacgcagc gcgcgcacca
     9961 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
    10021 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
    10081 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
    10141 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
    10201 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
    10261 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
    10321 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
    10381 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa atcattcggg tcggattgcc
    10441 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
    10501 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
    10561 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
    10621 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
    10681 aaatcgatgt gctgctttat caggacacca tcccgatcct gtctgaaaat ccagagatta
    10741 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
    10801 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
    10861 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
    10921 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttggtg ccgttgcagg
    10981 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
    11041 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
    11101 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
    11161 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
    11221 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
    11281 aaggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
    11341 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
    11401 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
    11461 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
    11521 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
    11581 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
    11641 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
    11701 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
    11761 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
    11821 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
    11881 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
    11941 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
    12001 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
    12061 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
    12121 accaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
    12181 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
    12241 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
    12301 gcgccacaac acattgctat atgttgttgg acaggataaa ccgcgaaaat ttgaggcgct
    12361 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
    12421 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
    12481 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
    12541 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
    12601 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
    12661 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
    12721 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
    12781 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
    12841 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
    12901 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
    12961 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
    13021 atggggtggc atttggcgaa aaaggcatga atccgctgac cagaacttca tttattatta
    13081 ccgaagatct gacaccaacc ataagtcttg aagattactg tgctgactgg gcgactaacc
    13141 ctccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcacgata
    13201 tgcatgctgc gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
    13261 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
    13321 ttcgcacgcg cgttccacgt cgttggcggg ataaagatct tattgggctt tatttttctt
    13381 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgccg
    13441 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
    13501 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta acccgtaact tatttttgcc
    13561 aaaattttgg atacagaata aatatgtctc aactcaatga tagtgacatc atcctttttg
    13621 agtataattt tcattatcaa aatataagat ctaaaaatac tcttgatata gcatttggta
    13681 ttgacagaaa ttttcttttt ggatgtggtg tagccatcgc atctattcta ttaaacaata
    13741 gagaaatctc ttgtgaattt catgttttca cagattatat cagtgataaa gacaaattat
    13801 atttttctga tttagca
//
LOCUS       DABSYT020000112        13610 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.112, whole genome shotgun sequence.
ACCESSION   DABSYT020000112 DABSYT020000000
VERSION     DABSYT020000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.112"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            232..1341
                     /gene="livK"
                     /locus_tag="ID725_RS19955"
                     /old_locus_tag="pgaptmp_003991"
     CDS             232..1341
                     /gene="livK"
                     /locus_tag="ID725_RS19955"
                     /old_locus_tag="pgaptmp_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGV7852480.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1389..2315
                     /gene="livH"
                     /locus_tag="ID725_RS19960"
                     /old_locus_tag="pgaptmp_003992"
     CDS             1389..2315
                     /gene="livH"
                     /locus_tag="ID725_RS19960"
                     /old_locus_tag="pgaptmp_003992"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGV7852481.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2312..3589
                     /gene="livM"
                     /locus_tag="ID725_RS19965"
                     /old_locus_tag="pgaptmp_003993"
     CDS             2312..3589
                     /gene="livM"
                     /locus_tag="ID725_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGV7852482.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3586..4353
                     /gene="livG"
                     /locus_tag="ID725_RS19970"
                     /old_locus_tag="pgaptmp_003994"
     CDS             3586..4353
                     /gene="livG"
                     /locus_tag="ID725_RS19970"
                     /old_locus_tag="pgaptmp_003994"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGV7852483.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4355..5068
                     /gene="livF"
                     /locus_tag="ID725_RS19975"
                     /old_locus_tag="pgaptmp_003995"
     CDS             4355..5068
                     /gene="livF"
                     /locus_tag="ID725_RS19975"
                     /old_locus_tag="pgaptmp_003995"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGV7852484.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5187..5933
                     /locus_tag="ID725_RS19980"
                     /old_locus_tag="pgaptmp_003996"
     CDS             5187..5933
                     /locus_tag="ID725_RS19980"
                     /old_locus_tag="pgaptmp_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGV7852485.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            6293..7609
                     /gene="ugpB"
                     /locus_tag="ID725_RS19985"
                     /old_locus_tag="pgaptmp_003997"
     CDS             6293..7609
                     /gene="ugpB"
                     /locus_tag="ID725_RS19985"
                     /old_locus_tag="pgaptmp_003997"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGV7852486.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            7707..8594
                     /gene="ugpA"
                     /locus_tag="ID725_RS19990"
                     /old_locus_tag="pgaptmp_003998"
     CDS             7707..8594
                     /gene="ugpA"
                     /locus_tag="ID725_RS19990"
                     /old_locus_tag="pgaptmp_003998"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGV7852487.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            8591..9436
                     /gene="ugpE"
                     /locus_tag="ID725_RS19995"
                     /old_locus_tag="pgaptmp_003999"
     CDS             8591..9436
                     /gene="ugpE"
                     /locus_tag="ID725_RS19995"
                     /old_locus_tag="pgaptmp_003999"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGV7852488.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9438..10508
                     /gene="ugpC"
                     /locus_tag="ID725_RS20000"
                     /old_locus_tag="pgaptmp_004000"
     CDS             9438..10508
                     /gene="ugpC"
                     /locus_tag="ID725_RS20000"
                     /old_locus_tag="pgaptmp_004000"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGV7852489.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10505..11248
                     /gene="ugpQ"
                     /locus_tag="ID725_RS20005"
                     /old_locus_tag="pgaptmp_004001"
     CDS             10505..11248
                     /gene="ugpQ"
                     /locus_tag="ID725_RS20005"
                     /old_locus_tag="pgaptmp_004001"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGV7852490.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALSARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11235..11675)
                     /locus_tag="ID725_RS20010"
                     /old_locus_tag="pgaptmp_004002"
     CDS             complement(11235..11675)
                     /locus_tag="ID725_RS20010"
                     /old_locus_tag="pgaptmp_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGV7852491.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            11795..13540
                     /gene="ggt"
                     /locus_tag="ID725_RS20015"
                     /old_locus_tag="pgaptmp_004003"
     CDS             11795..13540
                     /gene="ggt"
                     /locus_tag="ID725_RS20015"
                     /old_locus_tag="pgaptmp_004003"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGV7852492.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     CASDPRSVDDLTAGY"
BASE COUNT         3021 a         3455 c         3893 g         3241 t
ORIGIN      
        1 gttattttat gctgacaaag gcactttttt ctgtttatct atcaataaat tcagaatatt
       61 atctgttctt aatcgactga aaaataggga ctttaatcgc tattatcaca aaatactgcg
      121 ctaaaccctt aatcagacag gcaaaaacag tgcagtataa aaaaagaaca gtctgatttg
      181 ttaacacata aaaacaaagc aacacaacat cacgaatggg gatttttgac tatgaaacgg
      241 aatgcgaaaa ctatcatcgc agggatgatt gcactggcaa tttcacacac cgctttagcc
      301 gacgatatta aagttgccgt tgtcggcgcg atgtccggcc cgattgccca gtggggcgat
      361 atggaattta acggcgcgcg tcaggcgatt aaagacatta atgccaaagg gggaattaaa
      421 ggcgataagc tggttggcgt agaatatgac gacgcctgcg acccgaaaca agccgttgcg
      481 gtcgccaaca aaatcgttaa tgacggcatt aaatacgtta ttggtcatct gtgttcttct
      541 tctacccagc ctgcgtcaga tatctatgaa gacgaaggta ttcttatgat ctcgccggga
      601 gcgaccaacc cggagctgac ccaacgcggt tatcaacaca ttatgcgtac tgccgggctg
      661 gactcttccc aggggccaac ggcggcaaaa tacattcttg agacggtgaa gccccagcgc
      721 atcgccatca ttcacgacaa acaacagtat ggcgaagggc tggcgcgttc ggtgcaggac
      781 gggctgaaag cggctaacgc caacgtcgtc ttcttcgacg gtattaccgc cggggagaaa
      841 gatttctccg cgctgatcgc ccgcctgaaa aaagaaaaca tcgacttcgt ttactacggc
      901 ggttactacc cggaaatggg gcagatgctg cgccaggccc gttccgttgg cctgaaaacc
      961 cagtttatgg ggccggaagg tgtgggtaat gcgtcgttgt cgaacattgc cggtgatgcc
     1021 gccgaaggca tgttggtcac tatgccaaaa cgctatgacc aggatccggc aaaccagggc
     1081 atcgttgatg cgctgaaagc agacaagaaa gatccgtccg ggccttatgt ctggatcacc
     1141 tacgcggcgg tgcaatctct ggcaactgcc cttgaacgta ccggcagcga tgagccgctg
     1201 gcgctggtga aagatttaaa agctaacggt gcaaacaccg tgattgggcc gctgaactgg
     1261 gatgaaaaag gcgatcttaa gggatttgat tttggtgtct tccagtggca cgccgacggt
     1321 tcatccacgg cagccaagtg aaaatcccac cgcccgtgaa atgcgggcgg gtttagaaag
     1381 gttaccttat gtctgagcag tttttgtatt tcttgcagca gatgtttaac ggcgtcacgc
     1441 tgggcagtac ttacgcgctg attgccatcg gctacaccat ggtttacggc attatcggca
     1501 tgatcaactt cgcccacggc gaggtttaca tgatcggcag ctacgtctca tttatgatca
     1561 tcgccgcact gatgatgatg ggcattgata ccggctggct gctggtagcg gcgggattcg
     1621 tcggcgcaat cgtcattgcc agcgcctacg gctggagtat cgaacgggtg gcttaccgcc
     1681 cggtgcgtaa ctctaagcgc ctgattgcac tcatctcggc aatcggtatg tccatcttcc
     1741 tgcaaaacta cgtcagcctg actgaaggtt cgcgcgacgt ggcgctgccc agcttgttta
     1801 atggtcagtg ggtagtgggt catagcgaaa acttctctgc ctctattacc accatgcagg
     1861 cggtgatctg gattgtcacc ttcctcgcca tgctggcgct gacgattttc attcgttatt
     1921 cccgtatggg gcgcgcctgc cgtgcctgcg ccgaagatct gaaaatggcg agcctgcttg
     1981 gcattaacac cgaccgggtg attgctttga cctttgtgat tggcgcggcg atggcggcgg
     2041 tggcgggcgt actgctcggt cagttctacg gcgtcattaa cccctatatc ggctttatgg
     2101 ccgggatgaa agcctttacc gctgcggtgc tcggtgggat tggcagcatt ccgggagcga
     2161 tgattggcgg gctgattctg gggattgcgg aggcgctctc ttctgcctac ctgagtacgg
     2221 aatataaaga tgtggtctca ttcgccctgc tgattctggt gctgctggtg atgccgaccg
     2281 gtattctggg tcgcccggag gtagagaaag tatgaaaccg atgcatattg caatggcgtt
     2341 gctctctgcc gcgatgtttt ttgtcctggc gggcgtcttt atgggcgtac aactggagct
     2401 ggatggcacc aaactggtgg tcgacacggc ttcagacatc cgttggcagt gggtgtttat
     2461 cggcacggcg gtggtctttt tcttccagct tttgcgaccg gctttccaga aagggttgaa
     2521 aagcgtttcc ggaccgaagt ttattctgcc cgccattgat ggctccacgg tgaagcaaaa
     2581 gctgtttctc gtggcgctgc tggtgcttgc ggtggcgtgg ccgtttatgg tctcacgcgg
     2641 gacggtggat atcgccaccc tgaccatgat ctacattatc ctcggactcg ggctgaacgt
     2701 ggtggtaggg ctttctggtc tgctggtgct ggggtacggc ggcttttacg ccatcggcgc
     2761 ttataccttt gcgctgctca atcactatta cggcttgggc ttctggacct gtctgccgat
     2821 agccgggttg atggcggcgg cggcgggctt cctgctcggc ttcccggtgc ttcgcttgcg
     2881 cggtgactat ctggcgatcg ttaccctcgg tttcggcgaa attgtgcgca tattgctact
     2941 caataacacc gaaattaccg gcggtccgaa cggtatcagt cagatcccga aaccgacttt
     3001 tttcggcctt gagttcagcc gtaccgctcg tgaaggcggc tgggacacgt tcagtaattt
     3061 ctttggcctg aaatacgatc cctccgatcg tgtcattttc ctctacctgg tggcgttgct
     3121 gctggtggtg ctaagcctgt ttgtcattaa ccgcctgctg cggatgccgc tgggtcgcgc
     3181 ctgggaagcg ttgcgtgaag atgaaatcgc ctgccgttcg ctgggcttaa gcccgcgtcg
     3241 tatcaagctg actgccttta ccatcagcgc cgcgtttgcc ggttttgccg gaacgctgtt
     3301 tgctgcgcgt cagggctttg tcagcccgga atccttcacc tttgccgaat cggcgtttgt
     3361 gctggcgata gtggtgctcg gcggtatggg ctcgcaattt gcggtaattc tggcggcaat
     3421 tttgctggtg gtgtcgcgcg agttgatgcg tgatttcaac gaatacagca tgttaatgct
     3481 cggtggtttg atggtgctga tgatgatctg gcgtccgcag ggcttgctgc ccatgacgcg
     3541 cccgcaactg aagctgaaaa acggcgcagc gaaaggagag caggcatgag tcagccatta
     3601 ttatctgtta acggcctgat gatgcgcttc ggcggcctgc tggctgtgaa taacgtcaat
     3661 cttgaattgt acccgcagga gatcgtctcg ttaatcggtc ctaacggtgc cggaaaaacc
     3721 acggtcttta actgcctgac cggattctac aaacccaccg gcggcactat tttactgcgc
     3781 gatcagcatc tggagggttt gccggggcag caaatcgccc gcatgggcgt ggtgcgtacc
     3841 ttccagcatg tgcgtctgtt ccgcgaaatg acggtaattg aaaacctgct ggtggcgcag
     3901 catcagcaac tgaaaaccgg gctgttctct ggcctgttga aaacgccttc tttccgtcgc
     3961 gcccaaagtg aagcgctcga ccgcgccgcc acctggcttg agcgcattgg tttgctggaa
     4021 cacgccaacc gtcaggcgag taacctggcc tacggtgacc agcgccgtct ggagattgcc
     4081 cgctgcatgg tgacgcagcc ggagatttta atgctcgacg aacctgcggc aggtcttaac
     4141 ccgaaagaaa cgaaagagct ggatgagctg attgccgaac tgcgcaatca tcacaacacc
     4201 actatcttgt tgattgaaca cgatatgaag ctggtgatgg gaatttcgga ccgaatttac
     4261 gtggtcaatc aggggacgcc gctggcaaac ggtacgccgg agcaaatccg taataacccg
     4321 gacgtgatcc gtgcctattt aggtgaggca taagatggaa aaagtcatgt tgtcctttga
     4381 caaagtcagc gcccactacg gcaaaatcca ggcgctgcat gaggtgagcc tgcatatcaa
     4441 tcagggcgag attgtcacgc tgattggcgc gaacggggcg gggaaaacca ccttgctcgg
     4501 cacgttatgc ggcgatccgc gcgccaccag cgggcgaatt gtgtttgatg ataaagacat
     4561 taccgactgg cagacggcga aaatcatgcg cgaagcggta gcgattgtcc cggaagggcg
     4621 tcgcgtcttc tcgcggatga cggtggaaga gaacctggcg atgggcggct tttttgccga
     4681 acgtgatcag tttcaggagc gcataaagtg ggtgtatgag ctgtttccac gtcttcatga
     4741 gcgtcgtgtt cagcgggcag gcaccatgtc cggcggtgaa cagcagatgc tggcgattgg
     4801 tcgcgcgctg atgagcaatc cgcgtttgtt gttgcttgat gagccatcgc tcggtcttgc
     4861 gccgattatc atccagcaaa ttttcgacac catcgagcag ctgcgcgagc aggggatgac
     4921 tatcttcctc gtcgagcaga acgccaacca ggcgctgaag ctcgctgatc gcggctacgt
     4981 gctggaaaac ggccatgttg tgctctccga tactggtgat gcgctgctgg cgaacgaagc
     5041 agtgagaagt gcgtatttag gcgggtaaat tatcgataac ccggcagcta agttctgccg
     5101 ggttattcgt ccgtatacag acttgtttca actgaccggg aggattaaaa ccagaccact
     5161 tatttgccac ggaaaggttc cctcccatgc attatgccgt tgttgtcatg cttgaactgc
     5221 tttgctgtat tcacgcttac cgtaccggac aagagcggta ctggattttt atcattttct
     5281 gttttccggt gattggctgc gtggcgtatt tcgtcatagt gatgctgcct gaaacgggcg
     5341 ctgaccgtca cgggcatact ttgctgatgc gcctgcagga caaactcaac cctgagcgcc
     5401 acctgcgcaa attaaccgaa gaactggcga ttgccgagac gaatcaaaac cattatgcgt
     5461 tggctaatga actggcgcgt ctggggcgtt atcacgaagc tgttccccat tatcagcagg
     5521 cgttaagcgg gatattcgcc catgaagcgg caatgatgct aagcctggcg caggcacagt
     5581 ttgctattca ggagttcgcc gcctgtcagc agacgctgga agatgtgatg cgttataacc
     5641 ctgattttca gtcggcagac gggcatttgt tatttgccag aacgttagcc gcacaagaaa
     5701 aatacgctga tgcggaaagc gagtttgaag tattgatttc ttattacccc ggcccgcagg
     5761 cgcgtattta ctatgcggaa atgttggcga aaatgagtcg gttacgtgaa gccaacgagc
     5821 agtatgttgc cgtggttgat acggctaaac gctcaagacc tcattataga aaacaccacc
     5881 gggaatggat taagaccgcc aatgagcggc tgaagcagag tgtggtgcaa tgaaaatctt
     5941 ctttgccgtt agaccagtgt cgcggcaatc ttttgttaaa attgaaaaat gtagctctca
     6001 tcgggcagca aaattggacg cagaatttgc tgccattttt gctcttcatg gtaaggagtt
     6061 ctggcgatta gctggccgtt aatcgcctct tttcagtgca ttctcgggat atccgtgccc
     6121 catctcccat tttccctcac ccgtaccgtc accgccttgt catctttctg acaccttact
     6181 atcttacaaa tgtaacaaaa aagttatttt tctgtaattc gagcatgtca tgttaccccg
     6241 cgagcataaa acgcgtgaat tcgcgcattc ggtacaacaa gagagataaa cgatgaaacc
     6301 gttacattat acagcttcag cactggcgct cggactggcg ttaatgggga atgcacaggc
     6361 agtgacgacc attccgttct ggcattctat ggaaggggaa ctgggtaaag aggtggattc
     6421 tctggcccaa cgttttaacg ccgaaaaccc ggattacaaa attgtaccga cctataaagg
     6481 caattacgaa cagaatttaa gcgcggggat tgccgcattt cgtaccggca atgctccggc
     6541 tattttgcag gtttatgaag ttggcaccgc caccatgatg gcgtcgaaag ccattaaacc
     6601 ggtttatgac gtgtttaaag aggcggggat tcaattcgat gagtcgcagt ttgtgccgac
     6661 ggtttccggt tactactccg acagcaaaac gggccactta ctctcccagc cattcaacag
     6721 ctcgactccc gttctctatt acaacaaaga cgccttcaag aaagcggggt tagacccgga
     6781 acaaccgccg aaaacctggc aggatctggc ggactatgcc gcaaaactaa aagcctccgg
     6841 catgaagtgc ggctacgcca gcggctggca gggctggatc caactggaaa actttagcgc
     6901 ctggaacggt ctgccgtttg ccagcaaaaa caacggcttt gacggcacgg atgcggtgct
     6961 ggagttcaac aagccggagc aggtgaaaca catcgccatg ctcgaagaga tgaacaagaa
     7021 gggcgatttc agctacgtcg ggcgtaagga tgaatccacc gagaagttct ataacggtga
     7081 ttgcgctatg accaccgcct cttccggttc tctcgctaat attcgcgagt acgccaaatt
     7141 caactatggc gtaggcatga tgccttacga cgccgatgcg aaagatgcac cgcaaaacgc
     7201 cattatcggc ggggccagcc tgtgggtgat gcagggcaaa gataaagaaa cgtataccgg
     7261 cgtggcgaag ttcctcgact tcctcgcgaa gccagaaaac gctgccgagt ggcatcagaa
     7321 aaccggttat ctgccaatca ccaaagcagc gtatgacctg acccgtgagc agggctttta
     7381 tgagaaaaac ccaggggcgg ataccgcgac gcgtcagatg ctgaataagc cgccgttgcc
     7441 gttcaccaaa gggctgcgtc tgggcaacat gccgcagatc cgcgtgattg tggatgaaga
     7501 gctggagagc gtgtggaccg gtaagaagac accacagcag gcactggata ccgccgttga
     7561 gcgtggaaat cagttgctgc gccgctttga gaaatcgacg aagtcttaat cagtgtaatg
     7621 tcggatgcgt ttcgcttatc tgacctggca tcgcgtgtag gccggataag cgaagcgcat
     7681 ccggcacagt tcaggaatta accgtaatgt catcatcccg tccggtgttc cgctcgcgct
     7741 ggctgcctta tctgctggtc gcgccgcagc tcatcatcac cgttatcttt tttatctggc
     7801 ctgcgggcga agcgttgtgg tactcgctgc aaagcgtcga tccgtttggt ttctccagcc
     7861 ggtttgtcgg cctggataac ttcgtcgcgc tgtttcatga cagctactat atcgactcct
     7921 tctggacgac gataaaattc agcacctttg tcaccgtcag cggtttgctg gtatcgctgt
     7981 tctttgcggc gctggtggag tacatcgtgc gcggcagtcg tttctatcaa accttgatgc
     8041 tgctgccgta tgccgtggct cccgccgttg ccgccgtatt gtggatcttc ctgtttaacc
     8101 ccggtcgcgg gctgatcacc cattttctcg ctgagttcgg ctacgactgg aaccacgcgc
     8161 aaaacagcgg tcaggcaatg tttctggtgg tgtttgcctc agtatggaag caaatcagct
     8221 acaacttcct gttcttctat gccgcgctgc aatccattcc ccgttcgttg atcgaagccg
     8281 cagccatcga cggtgcaggg ccgattcgcc gcttctttaa gattgcgctg ccgcttatcg
     8341 ccccggtgag tttcttcctg ctggtagtga acctggtgta tgccttcttc gacaccttcc
     8401 cggtgatcga cgccgccacg tccggcgggc cggttcaggc caccactacg ctgatttata
     8461 agatctaccg cgaaggcttt accggactgg atctggcttc gtctgccgca cagtcggtgg
     8521 tgttgatgtt cctcgtcatc gtgctgacgg tgatgcagtt ccgctatgtt gaaagcaagg
     8581 tgcgttacca atgattgaga accgtccgtg gctgacgata ttcagccata ccatgctgat
     8641 cctcgggatc gcggtgatcc tattcccgct gtacgtggcg tttgtcgcgg cgacgctgga
     8701 taaacaggcc gtctacgccg cgccgatgac gctcatcccc ggcacgcatc tgctggaaaa
     8761 catccacaac atctgggtaa acggggtagg cacaaatagt gcgccgttct ggcggatgtt
     8821 gcttaacagc ttcgtgatgg cgttcagcat tacgctcggc aaaattaccg tctcgatgct
     8881 ctcggcattc gccattgtct ggtttcgttt tccgctgcgt aacctctttt tctggatgat
     8941 ttttatcacc ctgatgttgc cggttgaagt acgtatcttc ccgacggtgg aagtcatcgc
     9001 caacctgaag atgctcgaca gctacgcagg tttaacgctg ccgctgatgg cctcggcgac
     9061 cgcgacgttt cttttccgcc agttctttat gaccctaccg gatgaactgg tggaagccgc
     9121 gcggatcgac ggcgcgtcgc caatgcgctt cttttgcgac atcgtttttc cgttgtccaa
     9181 aactaatctg gcggcgttgt ttgtgatcac ctttatttac ggctggaacc agtatttgtg
     9241 gccgttgttg attattaccg acgtggatct cggcaccacc gtggcaggga tcaaagggat
     9301 gatcgccact ggcgaaggca ccacagaatg gaactcagtg atggcggcga tgttgttaac
     9361 cctgatccca ccagtagtga ttgttttagt gatgcagcgt gccttcgtgc gcggcctggt
     9421 cgatagtgag aaataagatg gcaggactga aattacaggc agtaaccaaa agctgggatg
     9481 gcaaaaccca ggtgattaaa ccgctgaccc ttgatgtggc ggatggcgaa tttatcgtga
     9541 tggtcgggcc gtcaggctgc gggaaatcga cgctgctgcg catggttgcc gggctggagc
     9601 gggtgacgga aggcgatatc tgtatcaacg accagcgggt gaccgaaatg gagccgaaag
     9661 atcgcgggat tgcgatggtg ttccagaact acgcgcttta tccgcatatg agtgttgaag
     9721 aaaacatggc gtgggggctg aaaattcgcg gcatgggcaa gcagcaaatt gccgagcgcg
     9781 ttaaagaggc ggcgcgcatt ctggaactgg acggtctgct taagcgccgc ccgcgcgagc
     9841 tttccggcgg tcagcgtcag cgtgtggcga tgggccgggc gattgtgcgc gatccggcgg
     9901 tgttcctgtt tgatgagcca ctctctaacc tcgatgccaa gctgcgcgta cagatgcgtc
     9961 ttgaactgca acagctgcac cgtcgcctga aaacgacttc actctacgtt actcacgatc
    10021 aggttgaggc gatgaccctc gcccagcgag taatggtgat gaacggcggc gttgccgaac
    10081 agattggcac accagttgaa gtctacgaaa agcccgccag cctgtttgtg gcgagtttta
    10141 ttggcagccc ggcgatgaat ctgctgacag gccgcgtgaa taacgaaggc acgcacttcg
    10201 agctggacgg cggcattgcg ctgccgctaa acggtggcta ccgtcagtat gcagggcgta
    10261 aaatgactct cggcattcgc ccggaacata tcgcgctaag ctcgcaggca gaaggcggcg
    10321 tgccgctggt gatggacacg ctggagatcc tcggcgcaga taacctggcg cacggacgct
    10381 ggggcgaaca gaagctggtg gtacggctgg cgcatcagga gcgcccgacg gcaggcagca
    10441 cgctgtggct gcatctgccg gaaaatcagc tacatctttt tgatggtgaa acaggacaac
    10501 gagtatgagt aactggcctt atccccgcat cgtcgcccac cgtggcggcg gtaaactggc
    10561 cccggaaaac accctggcgg caatcgacgt cggggcaaaa tacggtcata agatgatcga
    10621 atttgacgcg aagttgtcga aagatggcga aatcttcctg ctccatgacg acaatctcga
    10681 acgtaccagc aacggctggg gcgtcgcggg tgaactgaac tggcaggatt tactacgtgt
    10741 ggatgcaggc agttggtacg gcaaagcgtt taaaggtgag ccgctgccgt tgctttcgca
    10801 ggtggcggaa cgctgtcgcg aacatgggat gatggcgaat atcgaaatca aacccaccac
    10861 tggcactggg ccattaacgg gcaaaatggt ggcgctgtcg gcacgcgagc tgtgggccgg
    10921 tatgacgccg ccgctactgt catcgtttga gattgatgct ttagaagccg cacaacaggc
    10981 agtaccagaa ctgccgcgcg gtttgttgct ggatgagtgg cgcgacgact ggcgagaact
    11041 gaccgcgcgg ctgggctgcg tctctattca tctcaatcat aagttgctcg ataaagcgcg
    11101 agtgatgcag ttgaaagacg ccggattacg gattctggtt tataccgtca ataaacccca
    11161 gcgcgcggca gagttgctgc gctggggcgt ggattgcatc tgtaccgatg cgattgatgt
    11221 gattggtccg aactttacgg cccaatagtt ttcaacggta tatcaggctg cggcgtgccg
    11281 ggctgattca gcatgtcgcc gtttctcctc tccggcaaca tatgctgctg attcaacgaa
    11341 ctatccggat tacggttgct gtttaacatc ccgccattgg tgttgggcag catttgctgc
    11401 cgggcgggat ttcgctcccc cggctgtgac tgcaacaccc gctgagaatt attgtttatc
    11461 tggttttcta aatgctgttg ttgcaactga gtttgcgttt tcagttgctg attcagcatc
    11521 cctttttgct ggatttgctg cgtttgcatc tgggtttgca tccgctgctg actggggatc
    11581 tgataccccg gctggttagg gttgttcaga gtattaatgg gctgtgcaaa gccgacaaac
    11641 ggcaggagtg ccgtaagaat cagaagtcgt ttcatcgcgt atcctcctct gaagatattc
    11701 tttaagttta ctcctttcct gcaatcatca ggattaattc agagttatat accaggctta
    11761 gctggggttg ccccttaatc tctggagaat aacgatgata aaaccgacgt ttttacgccg
    11821 ggtggccatt gctgctctgt tcacaggaag ttgttttagt acggtagccg cgccacctgc
    11881 gccgtctcct gtttcttacg gcgtggagga agatgtcttt catccggtac gcgcgaaaca
    11941 ggggatggtg gcatctgtag atgctactgc cactcaggtg ggggtggata tcctcaagga
    12001 gggcgggaat gccgttgatg ccgccgtggc tgtgggctac gcgctggcgg taacgcatcc
    12061 gcaggcaggg aatctgggcg gtggtggttt tatgctgatc cgctcgaaaa atggcaatac
    12121 caccgctatc gatttccgcg aaatggcacc cgccaaagcg acccgcgata tgttccttga
    12181 tgaccagggc aacccggaca gcaaaaaatc actcacttcg catctggctt ccggcacgcc
    12241 cggtacggtc gctggtttct cgctggcgtt ggataaatac ggcacaatgc cgttgaacaa
    12301 agtggtgcag ccagcgttta aactggcacg cgatggtttt atcgttaacg acgagctggc
    12361 tgacgatctc aaaacctacg ggagcgaagt gctgccgaat cacgaaaaca gcaaggctat
    12421 cttctggaaa gagggcgagc cgctgaaaaa gggcgacaag ctggtgcagg cgaacctggc
    12481 aaagagcctg gagatgattg ccgaaaacgg cccggacgaa ttctacaaag gcacgatagc
    12541 ggaacagatt gcccaggaga tgcagaaaaa cggtggcctg atcactaaag aagatttagc
    12601 ggcgtataaa gcggtcgaac gcacaccgat aagcggcgat tatcgcgggt atcaggttta
    12661 ctccatgcca ccgccatcct ccggcgggat ccatatcgta caaatcctca atattctgga
    12721 aaacttcgat atgaagaaat acggttttgg cagcgccgac gcgatgcaga tcatggcaga
    12781 agcggagaaa tatgcctacg ctgaccgctc ggaatatctt ggcgacccgg attttgtgaa
    12841 agtcccgtgg caggcgctga ccaataaagc ctatgccaaa tctattgccg agcaaatcga
    12901 tatcaataaa gcgaaaccgt ccagcgagat tcgccccggc aagcttgcgc cttatgagag
    12961 taatcaaact acccattatt cggtggtgga taaagacggt aacgcggtgg cggtgaccta
    13021 tacgctgaac accaccttcg gtacgggtat tgtcgcgggc gagagcggta ttttgctcaa
    13081 taaccaaatg gatgatttct ctgccaaacc gggcgtaccg aacgtttacg ggctggtggg
    13141 cggtgatgcc aacgccgtcg ggccgaacaa acgcccgctg tcatcgatgt caccaaccat
    13201 tgtggtgaaa gacggtaaaa cctggctggt caccggtagc cctggcggga gcctgatcat
    13261 cactacagtg cttcaaatgg tggtgaatag catcgattat ggcatgaacg tcgccgaagc
    13321 gaccaatgcg ccgcgtttcc accatcagtg gttgccggac gagctgcgtg tcgaaaaagg
    13381 gtttagcccg gatacgctca agctgctgga agcaaaaggt cagaaagtgg cgctgaaaga
    13441 ggcgatgggc agtacacaaa gcattatggt tgggccggac ggtgagttgt actgcgcatc
    13501 cgacccgcgc tcggtggatg atttaacggc ggggtactaa ggttagcggc cctcttcgtg
    13561 ggaagagggc tgttttcttg aacaagataa attagtgtct ggattgcttt
//
LOCUS       DABSYT020000113        13520 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.113, whole genome shotgun sequence.
ACCESSION   DABSYT020000113 DABSYT020000000
VERSION     DABSYT020000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.113"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            411..1124
                     /gene="aphA"
                     /locus_tag="ID725_RS20020"
                     /old_locus_tag="pgaptmp_004004"
     CDS             411..1124
                     /gene="aphA"
                     /locus_tag="ID725_RS20020"
                     /old_locus_tag="pgaptmp_004004"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGV7852493.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1235..1651
                     /locus_tag="ID725_RS20025"
                     /old_locus_tag="pgaptmp_004005"
     CDS             1235..1651
                     /locus_tag="ID725_RS20025"
                     /old_locus_tag="pgaptmp_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGV7852494.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1655..2011
                     /locus_tag="ID725_RS20030"
                     /old_locus_tag="pgaptmp_004006"
     CDS             1655..2011
                     /locus_tag="ID725_RS20030"
                     /old_locus_tag="pgaptmp_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGV7852495.1"
                     /translation="MTISELLQYCMAKPGAEQSEHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2046..4868)
                     /gene="uvrA"
                     /locus_tag="ID725_RS20035"
                     /old_locus_tag="pgaptmp_004007"
     CDS             complement(2046..4868)
                     /gene="uvrA"
                     /locus_tag="ID725_RS20035"
                     /old_locus_tag="pgaptmp_004007"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGV7852496.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5123..5659
                     /gene="ssb1"
                     /locus_tag="ID725_RS20040"
                     /old_locus_tag="pgaptmp_004008"
     CDS             5123..5659
                     /gene="ssb1"
                     /locus_tag="ID725_RS20040"
                     /old_locus_tag="pgaptmp_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGV7852497.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5758..6039)
                     /locus_tag="ID725_RS20045"
                     /old_locus_tag="pgaptmp_004009"
     CDS             complement(5758..6039)
                     /locus_tag="ID725_RS20045"
                     /old_locus_tag="pgaptmp_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGV7852498.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6468..8054
                     /gene="pdeC"
                     /locus_tag="ID725_RS20050"
                     /old_locus_tag="pgaptmp_004010"
     CDS             6468..8054
                     /gene="pdeC"
                     /locus_tag="ID725_RS20050"
                     /old_locus_tag="pgaptmp_004010"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGV7852499.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(8057..8380)
                     /gene="soxS"
                     /locus_tag="ID725_RS20055"
                     /old_locus_tag="pgaptmp_004011"
     CDS             complement(8057..8380)
                     /gene="soxS"
                     /locus_tag="ID725_RS20055"
                     /old_locus_tag="pgaptmp_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGV7852500.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8466..8930
                     /gene="soxR"
                     /locus_tag="ID725_RS20060"
                     /old_locus_tag="pgaptmp_004012"
     CDS             8466..8930
                     /gene="soxR"
                     /locus_tag="ID725_RS20060"
                     /old_locus_tag="pgaptmp_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGV7852501.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9476..10825
                     /gene="ghxP"
                     /locus_tag="ID725_RS20065"
                     /old_locus_tag="pgaptmp_004013"
                     /gene_synonym="yjcD"
     CDS             9476..10825
                     /gene="ghxP"
                     /locus_tag="ID725_RS20065"
                     /old_locus_tag="pgaptmp_004013"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGV7852502.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10976..12625
                     /locus_tag="ID725_RS20070"
                     /old_locus_tag="pgaptmp_004014"
     CDS             10976..12625
                     /locus_tag="ID725_RS20070"
                     /old_locus_tag="pgaptmp_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGV7852503.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12779..>13520)
                     /gene="espX5"
                     /locus_tag="ID725_RS20075"
                     /old_locus_tag="pgaptmp_004015"
     CDS             complement(12779..>13520)
                     /gene="espX5"
                     /locus_tag="ID725_RS20075"
                     /old_locus_tag="pgaptmp_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HGV7852504.1"
                     /translation="YKTNFNFAIMEKILFDNCILDDSYFAQIKMTDGTLNSCSAMHVQ
                     FYNATMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYINLFRADLSFSKLDLINFKH
                     ADLSRVNLNKAILQNINLIDSKLFFTRLTNTFLEMVICTDSNMANVNFNNANLNNCHF
                     NCSVLTKAWMFNTRLYRVNFDEASVQGMGISILRGEENIPINSDTLVTLQKFFEEDCT
                     SHTGMSQTENNTHEVAMKITADIMQHAD"
BASE COUNT         3204 a         3269 c         3498 g         3549 t
ORIGIN      
        1 atatattcat ataatcatct ccttcgggag taataaaatt aatcaccatt gtagggtagg
       61 gttctggtca atcagaaatc atctttataa acttcgattg tttttgtaat gctgtatcat
      121 taagttcatt aaatcgtaca gcagataaat gttctatcaa atttcgctca tttgcagagt
      181 attcatcata ataaacgtaa aattaatgta tccttacatc gagcattaac cattttttac
      241 aaaaaaacga caggaacgta tttactaggt taaatataat catcctgctt ttcatcacaa
      301 aaaccgcaga taatccttcc tttcccctgt agctggcgtt atggtcagat agtttttgca
      361 acaaatctca caataaaaag tttcaacata ctgactattt agggaaaaat atgcgcaaga
      421 tcacacaggc aatcagtgcc gtttgcttat tgttcgctct aaacagttcc gctgttgccc
      481 tggcctcatc tccttcaccg cttaaccctg ggactaacgt tgccaggctt gctgaacagg
      541 caccaattca ttgggtttcg gtcgcacaaa ttgaaaatag cctcgcaggg cgtccgccaa
      601 tggcggtggg gtttgatatc gatgacacgg tacttttttc cagtccgggc ttctggcgcg
      661 gcaaaaaaaa cttctcgcca gaaagcgaag attatctgaa aaaccctgta ttctgggaaa
      721 aaatgaacaa cggctgggat gaattcagca ttccaaaaga ggtcgctcgc cagctgattg
      781 atatgcatgt acgccgtggt gacgcgatct tctttgtgac tggtcgtagc ccgacgaaaa
      841 cagaaacggt ttcaaaaacg ctggcggata attttcatat tcctgccacc agcatgaatc
      901 cggtgatctt tgcgggcgat aaaccagggc aaaatacaaa atcgcaatgg ctgcaggata
      961 aaaatatccg aattttttat ggcgattccg ataacgatat taccgccgca cgcgatgtcg
     1021 gcgctcgtgg tatccgcatt ctgcgtgcct ccaattctac ctacaaaccc ttgccacaag
     1081 cgggtgcgtt tggtgaagag gtgatcgtca attcagaata ctgacagagc gggagagcgt
     1141 gacactctcc cgaatgctgt ttttttaatc acacctttat cctttcgctg tcttgctgca
     1201 aactgattaa gagagtttta tcaaggagca gcacatgtgg tatcaaaaga cgctcacgct
     1261 tagcgccaaa tctcgtgggt ttcatctggt aacggatgaa attctgaatc agctggctga
     1321 tatgccgcgc gttaacatcg gcttactgca tctgttgctg caacatacct ccgcctctct
     1381 gacacttaat gagaactgcg atcccaccgt acgccacgac atggagcgtt ttttcctccg
     1441 caccgttccc gacaacggaa attatgagca tgactatgag ggagcagacg atatgccttc
     1501 tcatatcaaa tcctcaatgc tgggaacatc gcttgtattg ccggtgcata aagggcgtat
     1561 tcagaccggc acctggcaag gcatttggct gggggaacat cgcatccacg gcggatcgcg
     1621 tcgcatcatc gcgacactac aaggggagta aaaaatgacc atttcggagt tgctacaata
     1681 ttgcatggca aaaccaggcg cagaacagag cgagcataac gactggaaag cgacgcagat
     1741 caaagtggaa gatgtactgt ttgcgatggt gaaagaagta gaaaatcgcc cagctgtttc
     1801 gctgaaaacc agcccggagc tggcggagct gctacgtcag cagcacagcg atgtgcgtcc
     1861 aagccgccat ctgaataaag cgcactggag caccgtgtat ctcgacggtt cgctgccgga
     1921 ttcgcaaatc tattatctgg tggatgcgtc ttatcagcag gcggtgaatt tactgccgga
     1981 agaaaaacgt aaattgctgg tgcaactctg aaaggaaaag gccgctcgga aggcggcctt
     2041 aacgattaca gcatcggctt gaggaagcgt gccgtatgcg aagcttcgca ctccgcgacg
     2101 gtttctggcg taccggagac gaggatctcg ccgccgccac tgccgccttc cggccccagg
     2161 tcgacaatcc agtcagcggt tttaatcacg tcgagattgt gctcaatcac cacaatggtg
     2221 ttgccctgat cgcgcagttt atgcagtacg tcgagcagtt gctgaatatc ggcgaagtgc
     2281 agaccggtgg tcggctcgtc gagaatatac agcgtctgcc cggtgccgcg ttttgacagc
     2341 tcacgcgcca gcttcacgcg ctgggcttca ccaccagaaa gtgtggttgc ggactgcccc
     2401 aagcgaatgt acgtcagacc aacgtccatc aacgtttgca gcttacgcgc cagagctggc
     2461 accgcatcaa agaactcacg cgcctcttcg atggtcatat ccagcacttc gtggatggtt
     2521 ttgcctttgt acttaatctc cagcgtttca cggttatagc gtttaccttt gcactggtcg
     2581 cacggcacgt aaatgtccgg caggaagtgc atctccactt tgatcacgcc atcgccctga
     2641 caggcctcgc agcgtccgcc acggacgtta aagctgaaac gtcctggcgt ataaccacgc
     2701 gcacgggatt ccggtacgcc cgcaaacagt tcgcgcacag gcgtaaacac gccggtatag
     2761 gtcgccgggt tagaacgcgg agtacgacca attgggcttt ggtcgatatc gattactttg
     2821 tcgaaatgct ccagtccctg aatatcgcga tacggtgccg gttcggcgat ggtcgcacca
     2881 ttcaactggc gttgggcaat cgggaacagt gtgtcgttaa tcagcgtcga tttaccggaa
     2941 cctgaaaccc ctgtgatgca ggtaaacaga ccgactggca gcgttagcgt cacgtctttc
     3001 aggttgttgc cgcgtgcgcc agtcagctta agtacttttt ccggattcgc cggaacgcgt
     3061 ttcttcggca cttcaatctt gcgtttaccg ctcatgtact gtccggtcaa cgactccggc
     3121 accgccataa tcgcttccag cggaccttcc gcgaccactt caccgccatg tacacctgca
     3181 ccaggaccga tatcgatcac atgatcagcg gcgcgaatcg cgtcttcgtc gtgctccacc
     3241 acaatcacgg tattaccgag atcgcgcaga tggataagcg tacccaacag gcgctcgtta
     3301 tcgcgctggt gcaggccgat agacggctca tccagcacgt acataacgcc aaccaggccc
     3361 gcaccaatct ggctcgccag acggatacgc tgggcttcac cgccggaaag cgtttctgcc
     3421 gagcgggaaa gcgtcaggta attcaggccg acgttaacga ggaatttcaa acgatcgccg
     3481 atctctttaa gaattttttc cgcaatcttc gcccgctgac ctgcgagttt gagattgttg
     3541 aagaattcca tcgcatgacc aatgctcatg tcggagatgg cgggcagcgg cgtattctcg
     3601 acatacacgt ggcgcgcttc ccgacgcaga cgcgtccctt cgcagctggc gcacgggcga
     3661 ttgctgatga acttggctaa ttcttcacgt accgcactgg attctgtctc tttataacgg
     3721 cgctccatat tgtgcagcac gccttcgaac ggatgacgac ggatggaggt atcgccacga
     3781 tcgttcatgt atttgaattc aatgttttct ttgccagaac cgtacaacac cactttatgc
     3841 acgttcgcgc tcaggctgcc ccacggcgct tcgacgtcga acttatagtg atctgccagc
     3901 gatttcagca tctggaaata atagaagttg cggcgatccc agccacggat cgcaccacct
     3961 gccagcgaca gttccggatt ctggatcacg cggtcaggat cgaaatattg ctgtacgcca
     4021 aggccgtcgc aggtcgggca ggcacctgcc gggttgttaa acgaaaacag tcgcggctcc
     4081 agttcacgca tactgtagcc gcaaattggg caggcgaagt tggcggaaaa cagcagctct
     4141 tccgctttcg ggtcgtccat atccgccact accgcggtac caccggaaag ctccagcgcg
     4201 gtttcaaacg actcggcaag acgttgggta agatcgtcac gcaccttgaa gcgatcaacc
     4261 accacttcaa tagtgtgttt cttttgcagt tccagtttcg gcggatcgga aagatcgcag
     4321 acttcgccat caatacgagc acggatgtaa ccctggcttg ccaggttctc cagcgttttg
     4381 gtgtgttcgc ctttgcgctc tttaatgatt ggcgcgagca gcatcagacg cttgccttcc
     4441 ggctgcgaca gcacgttatc caccatctgg ctgacggttt gcgccgccag cgggacgtcg
     4501 tggtccggac agcgcggttc gccgacgcgg gcgtacaaca aacgcaaata gtcgtggatt
     4561 tcggtgattg tccccaccgt agaacgcggg ttatgagacg tcgatttctg ctcaattgag
     4621 atggcaggag aaagcccctc aatatgatcg acgtccggct tttccatcag tgacagaaac
     4681 tgccgcgcgt aggcggaaag ggattcaacg taacggcgct gcccttcggc atataaggtg
     4741 tcgaaagcga gcgaggattt gccagaaccc gaaagcccgg tcacgacaat gagcttgtcg
     4801 cgggggataa cgaggttgat gtttttgaga ttatgggtgc gggcgccccg aacttcgatc
     4861 ttatccattc acctttcccg gattaaacgc ttttttgccc ggtggcatgg tgctaccggc
     4921 gatcacaaac ggttaattat gacacaaatt gacctgaatg aatatacagt attggaatgc
     4981 attacccgga gtgttgtgta acaatgtctg gccaggtttg tttcccggaa ccgaggtcac
     5041 aacatagtaa aagcgctatt ggtaatggta caatcgcgcg tttacactta ttcagaacga
     5101 ttttttttca ggagacacga acatggccag cagaggcgta aacaaggtta ttctcgttgg
     5161 taatctgggt caggacccgg aagtacgcta catgccaaat ggtggcgcag ttgccaacat
     5221 tacgctggct acttccgaat cctggcgtga taaagcgacc ggcgagatga aagagcagac
     5281 tgaatggcac cgcgttgtgc tgttcggcaa actggcagaa gtggccagcg aatatctgcg
     5341 taaaggttct caggtttata tcgaaggtca gctgcgtacc cgtaaatgga ccgatcaatc
     5401 cggtcaggat cgctacacca cagaagtcgt ggtgaacgtt ggcggcacca tgcagatgct
     5461 gggtggtcgt cagggtggtg gcgctcctgc aggtggcaat atcggtggtg gtcagccgca
     5521 gggtggttgg ggtcagcctc agcagccgca gggtggcaat cagttcagcg gcggcgcgca
     5581 gtctcgcccg cagcagtccg ctccggcagc gccgtctaac gagccgccga tggactttga
     5641 tgacgatatt ccgttctgat ttgtcattaa aacaataggt tatattgttt taaggtggat
     5701 gattaaagca tctgccagcc ataaaaaaga agcctccgtt atggaggctt ctacgtatca
     5761 ggtcaaaatc attggccact gtggcggtgt ctgattcatc tcttctatca tcaccgactc
     5821 gatgttgtgc cagatggcgg tgatgtccgc cattgtgatg ccaagcaacc ccattgcgaa
     5881 ccagcaggcg gaaaccacgc ccagaccgct gctgatcact gcaagcccga cataatcaga
     5941 ccgacgaaaa cggatgttga gcgtgctggc cagaaacatc attacggcac taagaagttg
     6001 ccagcgaaga agaaccacgc cagtggtgag ggtagccatc aaacaattcc tctgaaaaga
     6061 gccgatgccc tggacagcgc ggcgttgttc acgggaggcg tggtacactc tggctatcgc
     6121 ggggcttgca gaacacaaaa atgaaacact ctgtttgttt cattaatttt gtgaactata
     6181 tcacaattga ttgtttgtta gccagattag gtcgtgactt ttattgctgt acagattatg
     6241 tggtttttca gtggcattaa gggtatatct tcccgccgcc tctgtattcc tgtagaaaat
     6301 taattttgaa tatcaatgaa ttattttcat ccaagtgacg attaaaaagg tatcaatttc
     6361 aaatcaggca aaagtgctat ttataccgta agatttatct aaagacgtcg gtagccaggg
     6421 tttcaccttg caatggccgg gtataaacag gcaggaaatt gatagcaatg aatcatcgtg
     6481 cacgacacca attactggcg ttgccgggca ttatcttttt agttctcttt cccatcattc
     6541 tttcgctatg gattgccttc ctttgggcaa aatcagaagt gaataatcag ctccgaacct
     6601 ttgctcaact ggcgttggat aaatccgagc tggtcattcg ccaggcagat ttagtgagcg
     6661 atgcagctga acgctatcag gggcaagttt gcactccagc ccatcaaaag cgaatgttga
     6721 atattattcg tggctatctt tatattaatg aattgatcta tgcccgtgat aatcattttt
     6781 tatgctcatc gctgatagtg cctgtaaacg gctatacgat tgtatcggcc gattataagc
     6841 gtgaacctaa tgtttctatc tattattacc gcgatacgcc ttttttctct ggctataaaa
     6901 tgacctatat gcagcgggga aattatgtgg cggttatcaa ccctctcttc tggagtgaag
     6961 tgatgtctga tgacccgaca ttgcaatggg gtgtgtatga tacggtgacg aaaacctttt
     7021 tctcgttaag caaagaggcc tcagcagcaa cgttttctcc gctgattcat ttgaaggatt
     7081 taaccgtacc aagaaatggc tatttatatg cgacagttta ttcgacaaaa cgcccaattg
     7141 cagccattgt tgcgacttca tatcaacgtc ttatagccca tttttataat catcttattt
     7201 ttgcgttgcc cgccggtatt ttggggagtc ttgttctgct attactctgg ctacgtattc
     7261 gacaaaacta tttgtctccc aaacgcaaat tgcaacgcgc cctcgaaaaa catcagctat
     7321 gcctttatta ccagccaata attgatatca aaacagaaaa atgtatcggc gctgaagcgt
     7381 tgttacgttg gcctggtgag caggggcaag taatgaatcc ggcagagttt attcctctgg
     7441 cagaaaagga ggggatgata gaacaggtaa ctgattatgt tattgataat gtcttccgcg
     7501 atctgggcgc gtaccttgca acacatgctg atcgctatgt ttctattaat ctgtcggcct
     7561 ccgattttca tacgtcacgg ttgatagcgc gaatcaatca gaaaacagag caatacgcgg
     7621 tgcgtccgca gcaaattaaa tttgaagtga ctgagcatgc atttcttgat gtcgacaaaa
     7681 tgacgccgat tattctggct ttccgccagg ctggttacga agtggcaatt gatgattttg
     7741 gtattggcta ctctaacttg cataacctta aatcattgaa tgtcgatatt ttgaaaatcg
     7801 acaaatcgtt tgttgagacg ctgaccaccc ataaaaccag tcatttgatt gctgaacata
     7861 tcatcgagct ggcgcacagc ctggggttaa aaacgatcgc tgaaggcgtc gaaactgagg
     7921 agcaggttaa ctggctgcgc aaacgcggcg tgcgctattg ccagggatgg ttctttgcga
     7981 aggcgatgcc gccgcaggtg tttatgcaat ggatggagca attacccgcg cgggagttaa
     8041 cgcgcgggca ataaaactac aggcggtggc gataatcgct gggagtgcga tcaaactgcc
     8101 gacggaaaac gcgggagaag gtctgctgag agacataacc caggtccatt gcgatatcaa
     8161 aaatcggacg ctcggtggtg cgcaactcaa cggcggccag taacagacgg cgttgacgaa
     8221 tgtaatcgcc aagcgtctga tgcgtcaccg tgcggaacat ccgttgcagg taccactttg
     8281 aatagcctga tttttttgcg acgacatcaa tgttaagcgg ctggtcaata tgctcgtcaa
     8341 tccatgcgat aagatcctga ataattttct gatgggacat aaatctgcct cttttcagtg
     8401 ttcagttcgt taattcatct gttggggagt ataattcctc aagttaactt gaggtaaagc
     8461 gatttatgga aaagaaatta ccccgcatta aagcgctgct aacccccggc gaagtggcga
     8521 aacgcagcgg tgtggcggta tcggcgctgc atttctatga aagcaaaggg ttgattacca
     8581 gtatccgtaa cagcggcaat cagcggcgtt ataaacgtga tgtgttgcga tatgtcgcaa
     8641 ttatcaaaat tgctcagcgt attggcattc ctctcgcgac cattggtgaa gcgtttggcg
     8701 tgttgcccga agggcatacg ttaagtgcga aagagtggaa acagctttcg tcccaatggc
     8761 gagaagagtt ggatcggcgc attcatacct tagtggcgct gcgtgacgaa ctggacggat
     8821 gtattggttg tggctgcctt tcgcgcagtg attgcccgtt gcgtaacccg ggcgaccgct
     8881 taggagaaga aggtaccggc gcacgcttgc tggaagatga acaaaactaa agcgccacaa
     8941 gggcgcttta gtttgttttc cggtctttgt ctttctctct atcccgctgg tacacaggag
     9001 ggtttccccc gacgtcaaca cacctcattc gagcacgtgg tggaggttcc ggttggtgtt
     9061 gatgctttaa ttgtatgtca ccgacgtttc ttcgccagtg taaaagtata ctttttagcc
     9121 gcaatatttt tgtcatctca gacgattttt tatcgcaatc ctcaacggta tacggctcga
     9181 taacgctgca atcttgcgca tcgacgataa cgtttgcgca tcaattgcct ggtttttcat
     9241 cgtcaagaca ataaaagaga aaaaagcagc aaacttcggt tgaaaaagcc gctatgatcg
     9301 ccggataatc gtttgctttt tttaccaccc gttttgtatg cgcggagcta aacgtttgct
     9361 tttttgcgac gcagcaaatt gtcgcaaacc tggagcagga agataacgtt tcgctggcag
     9421 gggattgtcc gccacgcatc ttgacgaaaa ttaaactctc aggggatgtt ctcttatgtc
     9481 tacgccatca gcgcgtaccg gcggttcact cgacgcctgg tttaaaattt cacaacgtgg
     9541 aagcactgtc cgtcaggaag tggttgccgg gttaacaacg tttctggcga tggtctactc
     9601 ggttatcgtc gttccaggca tgttgggtaa agcgggcttc ccgcctgcgg cagttttcgt
     9661 tgcgacctgt ctggttgccg gactcggttc tatcgtgatg ggtctgtggg ctaatctgcc
     9721 gttggcgatt ggttgcgcca tctccctgac ggcgtttacc gcattcagcc tggtgctggg
     9781 gcaacatatt agcgtacctg tcgcgctggg tgccgtgttc ctgatgggtg tgctgtttac
     9841 ggtaatttct gccacgggta tccgtagctg gattttgcgc aacttgccgc acggtgtggc
     9901 gcacggcacg gggatcggta tcggtctgtt cctgctgctc attgccgcta acggtgtcgg
     9961 tctggtgatt aaaaacccgc ttgatggtct gcccgttgcg ctgggtgatt tcgcgacctt
    10021 cccggtgatt atgtcactgg taggtctggc ggtgatcatc ggcctggaaa aactgaaagt
    10081 ccctggtggc attctgctga ccattatcgg tatctcaatt gtcggtttga tcttcgatcc
    10141 taacgtccat ttctccggcg ttttcgccat gccttcattg agcgatgaaa acggcaattc
    10201 actgattggc agcctggaca ttatgggcgc gctgaatcct gtagtcctgc caagcgttct
    10261 ggcgctggtg atgacggcag tatttgatgc caccggaact atccgcgctg tcgccggcca
    10321 ggcgaacctg ctggataaag atgggcagat catcgacggc gggaaagcac tgaccactga
    10381 ctccatgagc agcgttttct ctggcctggt gggtgcagct ccggcagcgg tatacatcga
    10441 gtctgcggcg ggtacggcgg cgggcggtaa aaccggtttg acggctatca ccgttggcgt
    10501 gctgttcctg ctgattctgt tcctctctcc gctttcttac ctcgttccgg ggtatgcaac
    10561 ggctccggcg ctgatgtacg ttggcctgct gatgctgagc aacgtggcga aaatcgactt
    10621 tgctgatttt gttgatgcga tggcgggtct ggttacggcg gtattcatcg tgctgacctg
    10681 taacatcgta acaggcatca tgatcggctt cgcgactctg gtgattggtc gtctggtttc
    10741 cggtgaatgg cgcaagttga acatcggtac ggtcgttatc gccgtggcgc tggtgacatt
    10801 ctatgcgggt ggctgggcta tctaatcttt catcggattc tgaaaacggg tggcaatggc
    10861 tgcccgtttt tattttctcc gcacattgtg tgagcttttg cgatattctg aaaaaatgag
    10921 aattcaggca taacgtcttt ctcagatcat aaaaaacatc gcaaacaggg aacgcatgga
    10981 aatcttcttc accatactga taatgaccct cgtggtctcg ctgtccgggg tggtcactcg
    11041 tgtcatgccc tttcagatcc cgcttccgct tatgcaaatc gccatcggtg cgctactggc
    11101 gtggccgacg tttggtttgc atgtggagtt tgatcctgaa ctctttttag tcttgtttat
    11161 cccgccgttg ctgttcgctg atggctggaa aacgccgacc cgtgaatttc tcgaacatgg
    11221 tcgagagatt ttcggcctcg cgctggcgct ggtggtggtc accgtggtcg gcattggctt
    11281 ccttatttac tgggtggtgc cgggcattcc gctgatcccc gcctttgcgc tggcggcggt
    11341 gctttctccg accgatgctg tggcgctctc cgggattgtt ggcgaagggc gcatcccgaa
    11401 aaaaatcatg ggcattttgc agggcgaagc gttgatgaat gacgcctccg gcctggtgtc
    11461 gttgaagttt gccgtggcag tggcgatggg gacgatgatc ttcaccgtcg gcggtgcgac
    11521 ggtcgaattt atgaaagtag ccattggcgg tattctcgcc ggttttgtgg tgagctggct
    11581 gtacggtcgc tcgctgcgat tcctcagccg ctggggcggt gatgaacccg cgacgcagat
    11641 cgtcctgctg ttcctgctgc cattcgcttc ttatctgatt gccgaacata ttggcgtttc
    11701 cggcatcctc gctgcggttg ccgccgggat gaccatcacc cgctccggtg tgatgcgccg
    11761 tgcgccgctg gcaatgcgcc tgcgtgcaaa cagcacctgg gcgatgctgg aatttgtctt
    11821 taacggcatg gtgttcctgc tgttaggtct gcagctgccg ggtattctgg agacgtcgct
    11881 gatggcagca gaaatcgacc ctaacgtcga aatctggatg ctgtttaccg atattattct
    11941 gatatatgcg gcgctgatgc tggtccgttt cggctggctg tggacgatga aaaagttcag
    12001 caaccgcttc ctgaagaaga agccgatgga gtttggttcg tggaccacac gagaaatcct
    12061 gatcgcgtct ttcgccgggg tgcgtggggc gatcactttg gccggtgtgc tctctatccc
    12121 gctgctcttg ccggatggta acgtcttccc ggcgcgctat gagctggtgt ttctggcggc
    12181 tggtgtcatt ctcttctcgc tgtttgtcgg cgtggtgatg ttgcctattc tgctacaaca
    12241 cattgaagtc gcggaccatt cgcaacaatt gaaagaagaa cgtattgcgc gagcggcaac
    12301 ggcagaagtg gcgattgtgg cgatccagaa aatggaggag cgtctggcgg cggataccga
    12361 agagaatatc gataaccagc tgcttacgga ggtcagttct cgcgtcattg gtaacctgcg
    12421 tcgtcgcgcc gatggacgta acgacgttga aagttccgtg caggaagaga accttgagcg
    12481 tcgcttccgt ctggcggcat tgcgttctga acgtgctgaa ctgtaccacc tgcgcgccac
    12541 gcgggagatc agcaacgaaa cgctgcaaaa attactgcac gatctcgatt tgcttgaagc
    12601 gttgctaatt gaggaaaatc agtaagccga aagcgacgta accaggcccg aggtaatctg
    12661 aaaaaggtgc aatcttttgc acctttaaca ggttagctcg ggctaacatt catgattcta
    12721 aaacaaataa gttatctctc ttttaaagtc atttcattgg ctgttcttcg acttaatctc
    12781 aatctgcgtg ttgcataata tctgcagtaa tcttcatagc tacttcatga gtattattct
    12841 cagtttgtga catgccagta tgagaggtac aatcttcttc aaagaatttc tgtagtgtta
    12901 ccagggtatc actattaatg ggaatattct cctccccacg gagaatggaa atgcccattc
    12961 cctggacgct agcctcatcg aaattaaccc gatagagacg ggtattaaac atccaggctt
    13021 ttgttaaaac agaacagttg aaatggcaat tgtttaaatt ggcattatta aaattaacat
    13081 tagccatatt agaatcggtg catataacca tttcgaggaa cgtatttgtt agccgtgtaa
    13141 aaaagagttt actatcaatt aagtttatat tctggaggat tgctttgttc agattgacgc
    13201 gagacagatc agcatgttta aagttaatta aatcaagttt agagaagcta aggtcggctc
    13261 taaataaatt aatatatggc gctattactt tataaagatt tacctcagcc atgtatgcca
    13321 tataaaaatt tgaataatca aggaaggtat ttttaatatt ggctctattc attgttgcat
    13381 tgtagaattg aacatgcata gcggagcatg aatttagagt tccgtcagtc atttttatct
    13441 gagcgaaata tgagtcatcg agaatacaat tatcaaaaag tattttttcc attatggcga
    13501 aattaaaatt cgttttatac
//
LOCUS       DABSYT020000114        13298 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.114, whole genome shotgun sequence.
ACCESSION   DABSYT020000114 DABSYT020000000
VERSION     DABSYT020000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.114"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            168..1112
                     /locus_tag="ID725_RS20080"
                     /old_locus_tag="pgaptmp_004016"
     CDS             168..1112
                     /locus_tag="ID725_RS20080"
                     /old_locus_tag="pgaptmp_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852505.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHVFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTEANKIL"
     gene            1194..1616
                     /locus_tag="ID725_RS20085"
                     /old_locus_tag="pgaptmp_004017"
     CDS             1194..1616
                     /locus_tag="ID725_RS20085"
                     /old_locus_tag="pgaptmp_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852506.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
     gene            2164..2613
                     /gene="glmS"
                     /locus_tag="ID725_RS20090"
                     /old_locus_tag="pgaptmp_004018"
     CDS             2164..2613
                     /gene="glmS"
                     /locus_tag="ID725_RS20090"
                     /old_locus_tag="pgaptmp_004018"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HGV7852507.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            2613..4001
                     /gene="glmL"
                     /locus_tag="ID725_RS20095"
                     /old_locus_tag="pgaptmp_004019"
     CDS             2613..4001
                     /gene="glmL"
                     /locus_tag="ID725_RS20095"
                     /old_locus_tag="pgaptmp_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HGV7852508.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            4020..5465
                     /locus_tag="ID725_RS20100"
                     /old_locus_tag="pgaptmp_004020"
     CDS             4020..5465
                     /locus_tag="ID725_RS20100"
                     /old_locus_tag="pgaptmp_004020"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HGV7852509.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            5465..6706
                     /locus_tag="ID725_RS20105"
                     /old_locus_tag="pgaptmp_004021"
     CDS             5465..6706
                     /locus_tag="ID725_RS20105"
                     /old_locus_tag="pgaptmp_004021"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HGV7852510.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMPMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            6710..8080
                     /locus_tag="ID725_RS20110"
                     /old_locus_tag="pgaptmp_004022"
     CDS             6710..8080
                     /locus_tag="ID725_RS20110"
                     /old_locus_tag="pgaptmp_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HGV7852511.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            8077..8394
                     /locus_tag="ID725_RS20115"
                     /old_locus_tag="pgaptmp_004023"
     CDS             8077..8394
                     /locus_tag="ID725_RS20115"
                     /old_locus_tag="pgaptmp_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HGV7852512.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            8555..9835
                     /locus_tag="ID725_RS20120"
                     /old_locus_tag="pgaptmp_004024"
     CDS             8555..9835
                     /locus_tag="ID725_RS20120"
                     /old_locus_tag="pgaptmp_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HGV7852513.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            9943..11595
                     /locus_tag="ID725_RS20125"
                     /old_locus_tag="pgaptmp_004025"
     CDS             9943..11595
                     /locus_tag="ID725_RS20125"
                     /old_locus_tag="pgaptmp_004025"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGV7852514.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            11605..12207
                     /gene="cobO"
                     /locus_tag="ID725_RS20130"
                     /old_locus_tag="pgaptmp_004026"
     CDS             11605..12207
                     /gene="cobO"
                     /locus_tag="ID725_RS20130"
                     /old_locus_tag="pgaptmp_004026"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGV7852515.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(12241..13146)
                     /locus_tag="ID725_RS20135"
                     /old_locus_tag="pgaptmp_004027"
     CDS             complement(12241..13146)
                     /locus_tag="ID725_RS20135"
                     /old_locus_tag="pgaptmp_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGV7852516.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
BASE COUNT         3617 a         3237 c         3156 g         3288 t
ORIGIN      
        1 cacttaaatg tatcgatgaa acaggtatga cttaccggga gtgattgttt ctgagtgaac
       61 agaggccact ttcgcctgat aaatgaaaaa tgagcgattt ttgcaagtta gtttttctat
      121 agccgagtac gctcagcgcc tcgcttgatt acaatgaggt tgctgctatg agtcctgata
      181 atatcggttc ggctgtcaat cgcttctcag gttggccatc taatgattct ctaaaaacag
      241 ataaagaatc aatgacatca cccgtgactg tgcaggtaat aaagatgatt gccgccatcc
      301 ttgacccaca tcggatagtc gttggaattg atacacttta tacaacgcag cgcgaaacat
      361 ttacctcaga gagtcaacag ctcattgaat cagcattgca aacacttcat gtgatgaatt
      421 taaataatct ggaaacaaat cgcgatgaag aaagtcacta tctggctttt gaatataacg
      481 ttgacacctc tgacagaata accgtttctg cttataatga aaaaaaacct ggctgctatg
      541 gccaatgtct tgtaatacag gatgacgaaa cagacttatt taacctgata aaaattaaaa
      601 ttttcgaagc caggcataat tatgtcgctc aattgatgag cgatccggaa tttatgttca
      661 aattcagtta tagcgctcaa caaaatagat tagaacctca tcttctggtt cccgcttatt
      721 tccctctaaa aacaaacgaa ccggttacgc aagaggatgt actgttactt tatcgtttct
      781 ttaaaatgaa tgatgatttt aataaattaa catcagatga atacatgagt atcctgacac
      841 ctctgatgaa gtgtgagcgt agcgtccacg acaacaacag gtatgtgacg ggcaaagata
      901 cccttcttct ggactatcct ccttctggta accagataca tttccatgtt ttccctgacg
      961 aatcagcaac actagtcctt tatttttcaa acaatgacat tgaatgtttc gtctttgaaa
     1021 gagatattcc gagccagtat cgctttttca aaatgtttac caatctggca ctggttatag
     1081 atcagttaac cgaagccaat aaaatacttt aattttctat atatccccta tggcaaatat
     1141 tgctaaacaa tgcctgggga ttaattttta ttactcacgg aaagatttaa ataatgatac
     1201 catctcagat ctctttcaat acccttccag tcaatgcaac atacgcttca gagacaacag
     1261 ttgacacaca aaaattctct gacatcctct actcagcggg gtgttcccta aaggatatgg
     1321 cttatctgag cagatgtctg gcatcaatac acccgactct cgccaaaaac atttatgaaa
     1381 cagaaaatct cagcgatcaa aaattactac atattgattg ccgtagtaca aatgaaatca
     1441 agataaatat cttttttggt caacaacgag aaggattaat tgaaattaat tctgacacag
     1501 tcattttttc gatattgtct tcattaattg actccgccat ttctaaattt caacaccaaa
     1561 tccccgttaa cggttcagtt aatcgtgaac tattgtatga ggattatact gattaaatcc
     1621 accctgtcgc gaaaaatggc ctgcgttatt gccagacgta ataaaacctt tgattaacgt
     1681 tactcatact gatagcacct tcatgtacct ctctttttta cgaaaaatta aaagagctaa
     1741 ctaaggtcat aattcgcatg aatcattaac catttgttat tttcaacatt atttttacat
     1801 taagcagaga tgaattgcag tgaaattcga cttaaatctt atgaaatgca tttactcatg
     1861 gctaaaacca cgtcacaaaa atgcaaatta gaagtagatc acaattatct caatacatta
     1921 aatcaagtta attttaaaaa tttaacactc atgacattag ttgacataac acactgaatt
     1981 aacatggaaa tttaaaaaca cacttcaaac aatcatttcc cgaaagacat tttgaaaata
     2041 gatcaattgc aacattacgg tcagataaag acactcctgt gatattaaac ctgtgccgtt
     2101 atgaacggcc ataaatacga cgtgtgtaac ttattttact ctctgtctta caaaggatta
     2161 cccatgaaga aagcaacact tgtgattggc gttattggcg cggactgcca tgcagtaggc
     2221 aataaagttc tggaccgcgt ttttagcaac cacgacttca gagtcattaa tctgggcgta
     2281 atggtcagcc aggacgaata tattgatgct gccattgaaa ctggcgctga cgcgattgtc
     2341 gtctcctcta tctatgggca tggcgatatc gactgtctgg gcatgcgcga acgctgcatc
     2401 gagcgtggac tgggggacat cctgctctat gtgggcggta acctggtggt cggaaaacat
     2461 gacttcgctg acgtggaaac caaattcaaa gagatgggat ttgatcgcgt ctttgcccca
     2521 agtcatgact tagaagatgt ttgccagctg atggcccacg atatcaacca acgtcatgac
     2581 gttgatactc gtattcttga agaggctatc tgatgcaaat cgtctctgtc gatattggat
     2641 cgacgtggac caaagcagcc ctctttaccc gggaggggga cgcgttaacg ttggttaacc
     2701 acgtactgac cccaaccacc acccatcatc tggcgaaggg gtttttctcc agcctgaacc
     2761 aggtgttaaa cgtagataat gccctccccc tgttaaacag cggtgaggtt gcattgaagt
     2821 attcctcctc tgccaaaggt gggctggcgg ttgccgcaat gggattggtg ccttctatca
     2881 ccctggaaac cgcgaaagta accgctcact ctgcgggtgc taaaattgcg caatattacg
     2941 cctacaagct gaaccgccgt gatatccagg cactggaaga aacgcagccc gatattctct
     3001 tgttcaccgg cggcaccgac ggcggagaag aaagctacgg actgaataac gcccgtgtgc
     3061 tggcagaatc caaactcgac tgcgccatta tttatgccgg aaaccgggat attcaggacg
     3121 aggtgcagga gatcctcggg cataaagacc tgaccattgt cgacaacgtt ctgcccgatc
     3181 ttgaccatcc gaaccctgta gctgcacgcc aggctatttg cgacatattc cttaaacgca
     3241 tcgttaaagg gaaagggctg gatgtcattg ttgataaaac aggcgaagaa ccaatgccga
     3301 caccatggac ggtctttgaa ctggtcaagg caatcagtaa cgtcgatcac agctggaaag
     3361 agttcatgct gatcgacatg ggcggcgcaa ccaccgatgt ttattccgcc tgtgccaaca
     3421 cgctctcgcc cgacaccgtt ttacacggcg taccggaacc cttcgtaaaa cggacggtgg
     3481 aaggcgatct gggaatgcgc gtctcggcag tggtggttgg tgaaagtgcg aaagagttag
     3541 tcaatgtgaa ttttgctcag caacccgcac agctggacgc tttttatcat tacctccgtc
     3601 atctagtcgc tcatcccgac tacttacccg caaatgcaga agagaaatat ttcgataccg
     3661 tcctggccgg actgtgtgtg ggttatgcca ccgaaagaca tgcgggcacc aaaaaagagg
     3721 tctgcacctg cgtcggcaat gtcgatttac agatgggacg tgacctgaca accgtgcgta
     3781 aagtcgtcgg atccggcgga tggctctcgc gtgccagtca gttcgatatg catcactggt
     3841 tgaaataccg cgagttaaac gacgacggta aacgcattct cttaccgacc gaatttgaat
     3901 attaccgcga ttcccggggg ctattaccac tgctggcaaa cgtcgccaga gtcgatccgc
     3961 tggccgccgc gcgcaccagt attcaatgct taaccctata acaattaagg ctgcaatcaa
     4021 tggaacttcg aaataagaaa ttaacccatg acgaattcat gaccgagcgg caacaggtac
     4081 tgaaaacctg ggaaaccggc aaggatgttg aaaactttga agatggcgtt aaataccaac
     4141 aaaccatccc tgagcataaa cgtttctctc ttgcgctgct gaaagcagac aaggaaggta
     4201 aaacccttag ccaaccgcgc gcaggtgtgg cgctgatgga cgaacacatc gaactgctga
     4261 aaacactaca agaagagtgc gatctgctgc caagcaccat cgatgcatat acccgtctga
     4321 atcggtacga agaagcggct gtagggatta aaaaatccat cgaagccgga acctctaaac
     4381 tgaacggttt accggtagtt aaccatggtg tcgcagcttg ccgtcgcctc accgaaaccc
     4441 tgcaaaaacc gctacagatc cgccacggca cgccagatgc gcgtctgctg gctgaaatct
     4501 caatggccag cggctttacc agttatgaag gcggtggcat ttcctacaac attccgtatg
     4561 ccaaacgcgt aacgctggaa aaatcgatcc gcgactggca gtactgcgac cgtttaatgg
     4621 ggatgtatga agaacacggg atccgcatca accgcgaacc gtttggccca ctaacgggta
     4681 cgctgatccc gccgtttatc tcgcactcta tcgcgataat cgaaggcttg ctggcgctgg
     4741 aacagggtgt gaaatctatc accgttggtt atggccaggt cggcagcctg acgcaggatg
     4801 ttgcggcgat ccagtctctg cgtgaacttg cacacgaata ttttcagagc tatggctata
     4861 ccgactatga attgagtacc gtgtttcacc agtggatggg cggctttccg gaagacgaat
     4921 cgaaagcgtt cgctattatc tcctggggcg cagccgtcgc ggggatgtct ggtgccacca
     4981 aagtcatcac caaaagtccg cacgaagcct ggggtatccc gactgccgct gccaacattc
     5041 aggggctgaa agcctcccgc caaatgctca acatggtcaa tgagcagaaa ttcccgccgt
     5101 gccctgcggt tgaactggaa attgaactga ttaagagcga ggttcgcgcg gtactgaata
     5161 aagtgttcga actgggtaat ggtgatattg cgcgcggaac ggtgctggca tttgaagcgg
     5221 gcgtactgga tgtgccgttt gcgccagccg cctgcaacgc gggcaaaatt cttccggtcc
     5281 gtgacaacac cggcgcgata cgcgtactgg aagccggagc cgttccattg ccaaaagata
     5341 ttctcgacct ccaccacgat tacgttgcgg aacgcgcacg ctgtgaagga cgccaaccga
     5401 cattccagat ggttgtagat gacatcaatg ctgtatccca cagtaaatta ataggaagac
     5461 cataatgaaa ataaaacagg ctctgttcac cgctggctac tcctcattct atttcgatga
     5521 ccagcaggcg ataaaaaacg gagcgggtca tgacggcttt ttttataccg gggagccagt
     5581 aacacagggg tttaacgccg tacgtcaggc cggggagtgc gtatcggtac agttgattct
     5641 ggaaaacggc gcggtcgccg taggtgactg tactgccgta cagtattccg gggcaggcgg
     5701 tcgcgatcca ctgttcctcg cagagcactt tattccgttc ctcaacgacc atatcaagcc
     5761 attactggta ggccgcgatg tggatgcttt cctgccgaat gcccgtttct tcgacaaatt
     5821 gcgtattgac ggcaacttgc tgcataccgc cgtgcgctac ggattatcac aggcgctgct
     5881 tgatgctacc gcgctggcaa ccggccgtct gaaaactgaa gtggtctgtg atgaatggca
     5941 gttgccacgc gtggcggaat ccattccatt atttggtcag agcggcgacg atcgatatat
     6001 cgccgtcgat aagatgatcc ttaaaggcat cgacgtgctg ccccatgcgc tgattaataa
     6061 cgtcgaagag aagctgggct ttaaaggtga aaaactgcgc gaatatgtcc gctggttgtc
     6121 ggatcgcatt ctaagcaagc gcaccagcgc acgctaccac cctaccctgc acatcgatgt
     6181 atacggcact atcggtctga tcttcgatat ggatccgctt cgctgtgcgc aatacatcgc
     6241 cagcctggaa aaagaagcgc aaggcctgcc gctctacatc gaagggccgg tcgatgccgg
     6301 taacaagccc gatcaaattc gcctgctgac cgcgattacc aaagagctga cgcgcctcgg
     6361 ttccggcgtg aaaattgtgg ccgatgaatg gtgtaacacc taccaggata ttgttgattt
     6421 cactgatgct gccagttgtc acatggtgca aatcaaaacc ccggatctgg gcagtattca
     6481 caacatcgtc gatgcggttc tttactgcaa cagccacagc atggaagcgt accagggcgg
     6541 cacctgcaat gaaactgatg tcagtgcccg cacctgtgtc cacgtcgccc ttgccgctcg
     6601 ccccatgcct atgctggtaa aaccagggat gggctttgac gaaggcctcg atatcgtctt
     6661 caacgaaatg aatcgtacta tcgcgctgtt gcaggctaag gattaacaaa tggctcgttc
     6721 ttttaaagtc ttatcgccaa cggcaatcct cgggtatgga ttcccggaag agagcttccg
     6781 caaagcgatg gcagaatcgc ccgatctgat cgccgttgat gcaggctctt ccgatccagg
     6841 cccccactac ctcggggcag gcaaaccgtt taccgatcgc gcaggggtca agcgcgatct
     6901 gcgctatatg atcaccgcag gcgtgcaaaa taatataccg gtggtgatcg gaacggctgg
     6961 cggttctggt gccgcaccac acctggagtg gtgccgagaa ataattcatg agattgcccg
     7021 ggaagagcac ctctcgttct cgatggcgtt gatcccggca gacgttgaca aagccatcgt
     7081 ccaccaggcg ctggataacg gcaaaatcac ggcgctggat tttgtcccgc cgttaaccca
     7141 cgacgcgatt gacgaaagta cgtatatcgt cgcgcaaatg ggcatcgaac ccttccagcg
     7201 ggcgctgaaa gaacgcgcgc aagtggtgct gggcgggcgc gcttacgacc cggcctgctt
     7261 tgctgcgctc ccgatcatgc aaggctttga tgaaggtctg gcgctgcatt gtgggaaaat
     7321 ccttgaatgc gcggcaatcg ccgcaacgcc cggctcaggc tctgactgtg cgatgggcat
     7381 cattgatgac aacggcttta cgctgaaaac atttaatccg aagcgtaaat ttaccgaaac
     7441 gtcagcagct gcacacacac tgtatgagaa atccgatccc tacttcctgc caggacctgg
     7501 cggcgtgttg aacctgaaag gctgcacatt taaagcagta aatgacggcg aagtctacgt
     7561 cagcggttct aagcatgaag aaacgccgta tgccctgaaa ctggaaggtg cacgacgggt
     7621 gggcttccgc tgtctgacca tcgccggaac gcgcgacccg attatgatcg ccgggatcga
     7681 taaaatcatc gatgaagtca aaaccagcgt ttcgcgtaac ctgtcgctcg acgatgacag
     7741 cattcgcatc aatttccacc tgtacggcaa aaacggggtg atgggagacc atgaaccgat
     7801 gcaaactgcc gggcatgagc tggggattgt ccttgatgta gttgcaccga cccaggaaat
     7861 tgccaacagc gtttgctcgc tggtgcgctc taccatgctg cactacggct atgaaaaccg
     7921 catcgctacc gcaggtaatc tggcgttccc gttctcccct tctgatatcc agggcgggcc
     7981 ggtatacgaa ttttccatat atcacctgat tgaagccaac gacgctctgc gttttgattt
     8041 ccatattgaa caggtgacgc cagaaggagt tcaggcatga aacagtcaat tgtctcactg
     8101 gcgcaagtca ttcgttcgaa aaatgccgga ccttacgaac tggttttaga tattttattt
     8161 aaaacgaaag aaaactatga gcgagttaaa tcatcagggc aattaacccc agaactgatt
     8221 gcacgcttat atcatgttga acctgacttt attcatcgta ttgtctggtt tgatccttct
     8281 aacgcagtaa aaatcgtcat gccacgagat attatctccg ggaatgtagg tgacaatgat
     8341 gtctatggtg cacagcaaca tgcaccttta ttaaatatga gtttcgattt gtaaaaatcc
     8401 gaatactttt agccaactga catcccgtca cttattgcag ttcaacactt cagctatttg
     8461 taaggttgta atagcaggta gatgcctgaa tctctattca ggcatctact tctcctgatt
     8521 ctgatttatc gccgtgcacc tgatggagga aaaaatgaag aaaataagtt taaccacgat
     8581 gattcttttg gcgctggtac ttggaatgat tatcggcgta gtgctcaata acactgcttc
     8641 accggaaacc gcaaaactct atgcgcaaga aatatcgata ttcacgacga ttttcttacg
     8701 actgataaaa atgattatcg ctccgttagt ggtctctacc ctggtggtag gtattgctaa
     8761 aatgggcgat gccaaagccc ttggtcgtat tttttctaaa acactctttt tatttatttg
     8821 cgcctcattg ctgtcaattg ccttaggctt gataacggta aatttcttca tgccaggtac
     8881 aggaattaat tttgttgcac acggtgccga aaccaccgga gtggtcgcgg cagaaccctt
     8941 tacgctaaaa gtatttattt cgcatgcttt ccccaccagc attgtcgatg ccatggcgca
     9001 caatgaaatt ttgcaaatcg tggtgttctc aattttcctc ggctgtagcc tgacggcgat
     9061 tggcgagaaa ggcagcgcca tcgttcacgc cttagattcg ctggcacatg ccatgttaaa
     9121 gctcactggc tacgtcatgc tcttcgctcc cctgaccgta ttcgctgcta tttcagcatt
     9181 gattgctgaa cgaggactgg cagttatggt gagcgccggg atctttatgg gtgaatttta
     9241 tttcaccatg ttattacttt gggtactgct tatcggtctg gccatcgttt atgtcggccc
     9301 ctgcatcaga cgcctgaccc gcgccctttc ggaacccgcc ctgctggcat ttaccacatc
     9361 cagttctgaa gcggcttttc cgggaacgct tgaaaaactg gagcaatttg gcgtttcccc
     9421 caaaattgcc agctttgtct tacccattgg ctactcattt aatctcgttg gatcaatggc
     9481 ctactgctcc ttcgccacag ttttcatcgc ccaggcctgc aatatccatt tatccatcgg
     9541 tgagcaaatc accatgctgt tgatcctgat gttgacctcg aaaggaatgg ctggcgtacc
     9601 acgcgcctca atggtggtta tcgccgccac gctcaaccag ttcaatattc cggaagcggg
     9661 gctgatcttg ctgatgggcg ttgatccgtt ccttgatatg gggcgttccg cgacaaacgt
     9721 catgagcaac gcaatgggcg ctgcgatggt gagtcggtgg gaaggcgaac atttcggcga
     9781 gggctgtcgg ggtaaagcat taaaacccaa tgaatcgaac gttgctctgc cctgaggggg
     9841 atcttaagca atcagaagcc aatgactacg catttttata agccggatca tgaagttaga
     9901 cggcaattaa gtaacactaa cgtttaaggt gaaaagtgaa atatgtccaa accatttatc
     9961 tggcaagaac cgtttctaca aaataaagac ggtacagaat atacattaat tagcgatcaa
    10021 catattacag taacggaatt agacggcgaa gaagtgataa aaatcgctcc tgaagcatta
    10081 acgctgcttg cccggcaggc attttatgaa gcctcttttt tcttacgttc cgctcatttg
    10141 caacaggtcg ccagcattct taacgatcag caggccagca gtaatgataa atacgttgcg
    10201 ttgcagttat tacgtaatgc tgaagtctca gcaaaagggg tattacccaa ctgtcaggat
    10261 accggcaccg caactattgt tgccagtaaa gggcaacaga tatggaccgg aggtaacgac
    10321 gccgaagcct taagtaaagg catttacagt actttccagg aaaataatct gcgcttttca
    10381 caaaatgcgc cgctggatat gtataccgaa gtcaatacgc aaaccaatct cccggcgcag
    10441 attgatatca gcgctgtcgc tggtgatgag taccattttc tctgtgttaa taaagggggc
    10501 ggctccgcca ataaagccgc gctttatcag gaaaccaaat ccttattgca accagaaaaa
    10561 ctgaccgcat ttttaatcga aaaaatgaaa tcgctgggca ccgctgcctg cccgccttac
    10621 cacatcgcct ttgtcgtcgg agggctttca gccgatcaaa ccttaaaaat tgccaagctg
    10681 gcctcaacga aatattacga caatttgccc accagcggaa acgagcaggg acaagcattc
    10741 cgtgatattg agctggaaaa agtgttgctc gaggccagtc agcaatttgg tattggtgcg
    10801 caatttgggg gtaaatattt cgctcatgat attcgcgtca ttcgtctgcc gcgtcacggt
    10861 ggctcttgcc cgatagctat ggcgctctcc tgttctgccg atcgcaatat caaagcaaaa
    10921 attaacaaac acggtatctg gctggaaaaa ctcgaacaca atccggggca atatattcca
    10981 gcctcactgc gggaagaaaa tcacgcgcaa catgtacaac tggatctaaa ccgtccgttg
    11041 cgtgacgtga tgcaggatct cgccagatta ccagtaggca cccgcgtgtc gttaagtggt
    11101 cccatcgtag tggcgcgcga tatcgcccac gccaaaataa aagcgcggct ggatagcggc
    11161 gaaccgatgc cggagtacct gaagcatcat attgtctatt acgctggccc ggcgaaaaca
    11221 ccggagaaca tggcctgcgg ttcactgggt ccaaccacgg gcggtcgaat ggacggttat
    11281 atcgatacct tccaggcggc tggcggcagt ctagtgatgt tatcgaaagg taaccgcagc
    11341 cagcaggtca ccgatgcctg tcacaaacat ggcggcttca atctgggaag tattggcggt
    11401 gcagctgcgt tactggcgca agaatatgtc aaaagtctgc gttgcctgga atacccggag
    11461 ctgggtatgg aagcggtgtg gatgatggaa gtggaaaatc tgcccgcatt catcctggta
    11521 gatgataaag gcaataattt ctttagtcag tttgagcagc aacaccgctg cgccagttgc
    11581 cctgcgggac attaaggaaa atgaatggaa gcgcgaattt caactgaacg gcatgtgcag
    11641 cgccaacaga aattgaagga acaggtcgat acgcgcgtcg cggcggcgat agagaaaaaa
    11701 gggatcttga ttgttttcac cggcaacggc aaagggaaat ccactgccgc gtttggtact
    11761 gtgacgcgcg cggtgggtca tggcaaaacg gtgggggttg cgcagtacat caaggggcaa
    11821 tgggacaacg gcgaatacaa ccttctccag ccgctggggg ttgagtttca tattatggga
    11881 accggtttta cctgggagac acagaaccgg caagcggata tcgacgcggc gaaagaggtc
    11941 tggtcggaaa gcaaacggat gctggctgac aagcgttacg acttagtggt gcttgatgag
    12001 ctgacctata tgctggctta ccattatctg gataccgaag aagtgatcgc ttctctgcaa
    12061 aaccgcccgg cacaacaaag tgtcattgtc accggacgcg gttgccacag tcagatatta
    12121 aaaatggcag atacggtcag cgaaatccgc cccgtcaaac atgcttttga taacggcatt
    12181 caggcccagc cgggtatcga ctggtaataa ctgcgcctgg catcccgcca ggcgcaggtt
    12241 ttacatctcc tgcggtacga ccgatttcgc cagcaaccat tcacgaaatt gctgcaagtg
    12301 cggtgagtca gacttctctt cctgccacgt cataataaaa cgattaccgg tacgaatagg
    12361 cacatcacag ggaatcacca tatcgccact atccaaatca tgttgaatag cgaaacgtgg
    12421 taacagcgcc acgccgagat tagagcgcac ggcggcaatc agcattgaaa gcaaatcgaa
    12481 acgtggtccg ttgttgacca gcggactgct aacgccagat aaagcaaacc actcttccca
    12541 gccagtaata cgggtgcttt gatgcagaag tggtaactca gtaagcagtt ctgccactga
    12601 cagtttttga tcggacattg caagcaaact gccgctgcat accggcaaaa tctcctcttc
    12661 aaagagatat tctacttccg cccagggcgc gcaaaaattt tcgcgcataa tgacagcatc
    12721 gtactcccga ttaagaaaat cgccgttatt agctaaagaa tgaatattaa caataatatc
    12781 gggatgtaac tttgtgaatt catgtaaatt tgggattaac cagtgggtac taaaagtagg
    12841 attaacggca agttcaataa cctgaaccgt aggctgccag gtcataatgg tattggtatc
    12901 gcgttccagc ttatttaatg tttcttttac tatccccagg taatacttcc ctgtatcatt
    12961 aagaaaaatg cgttttttag agtgtgaaaa taacggagta cgtaaaaatt cctccagcgc
    13021 acttacttgt ctgaatactg cactttgggt taacgctaac tcctctgccg ctctggtata
    13081 actttcatga cgtgcgacga cctcaaaagt caccagcagt tccgttttag gtattttccc
    13141 tcgcataatg ccgtccctta aaaatagttt aaaccatcaa aaatacatta cttattacga
    13201 agtaatggta accaaagcag caataaataa tttataagaa tcatgattga catttataaa
    13261 ccagtaaggc tggcgtgaca aatatcttaa tcagataa
//
LOCUS       DABSYT020000115        13158 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.115, whole genome shotgun sequence.
ACCESSION   DABSYT020000115 DABSYT020000000
VERSION     DABSYT020000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.115"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(56..1468)
                     /locus_tag="ID725_RS20140"
                     /old_locus_tag="pgaptmp_004028"
     CDS             complement(56..1468)
                     /locus_tag="ID725_RS20140"
                     /old_locus_tag="pgaptmp_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852517.1"
                     /translation="MSYALIDNASLTAVERTLGDILVKNPDTINGDLVAFENLIQAIL
                     FYDTLICVDNYKKEYRDKRIAKFDFIKFVSESDFQLSELDQLAQVESRQITSEIRGGE
                     FVDDDFRQLVEMLKLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASIFNELS
                     DASDTKGYWSTDVKLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASLNWLAY
                     KSIYYSVIAKHFKADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKISTARSE
                     IVDHGRTSTISLDLPIFSAWLANETGNVSQVINSALELRTHDHFRTCRDVIREIHVTY
                     DESGISAGNKKVTKLLSDLDKISGDIKRSYGVPSNQGIQGSFLIKCINSLTGLVGIPG
                     LPDKEFALSTPEFMKSKQHKAFSTVFKDVTNELTSIERLGGLRDKLASNFTIDDSYYT
                     EPKTEDPRFRYVSSNWKQPM"
     gene            complement(1655..1831)
                     /locus_tag="ID725_RS20145"
                     /old_locus_tag="pgaptmp_004029"
     CDS             complement(1655..1831)
                     /locus_tag="ID725_RS20145"
                     /old_locus_tag="pgaptmp_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852518.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            2134..2760
                     /locus_tag="ID725_RS20150"
                     /old_locus_tag="pgaptmp_004030"
     CDS             2134..2760
                     /locus_tag="ID725_RS20150"
                     /old_locus_tag="pgaptmp_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGV7852519.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
     gene            3111..3278
                     /locus_tag="ID725_RS20155"
                     /old_locus_tag="pgaptmp_004031"
     CDS             3111..3278
                     /locus_tag="ID725_RS20155"
                     /old_locus_tag="pgaptmp_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGV7852520.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(3358..4605)
                     /locus_tag="ID725_RS20160"
                     /old_locus_tag="pgaptmp_004032"
     CDS             complement(3358..4605)
                     /locus_tag="ID725_RS20160"
                     /old_locus_tag="pgaptmp_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HGV7852521.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4677..5591)
                     /locus_tag="ID725_RS20165"
                     /old_locus_tag="pgaptmp_004033"
     CDS             complement(4677..5591)
                     /locus_tag="ID725_RS20165"
                     /old_locus_tag="pgaptmp_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGV7852522.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5807..7240
                     /gene="cscA"
                     /locus_tag="ID725_RS20170"
                     /old_locus_tag="pgaptmp_004034"
     CDS             5807..7240
                     /gene="cscA"
                     /locus_tag="ID725_RS20170"
                     /old_locus_tag="pgaptmp_004034"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGV7852523.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(7248..8243)
                     /locus_tag="ID725_RS20175"
                     /old_locus_tag="pgaptmp_004035"
     CDS             complement(7248..8243)
                     /locus_tag="ID725_RS20175"
                     /old_locus_tag="pgaptmp_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGV7852524.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            8486..8704
                     /locus_tag="ID725_RS20180"
                     /old_locus_tag="pgaptmp_004036"
                     /pseudo
     CDS             8486..8704
                     /locus_tag="ID725_RS20180"
                     /old_locus_tag="pgaptmp_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8722..10050
                     /gene="dsdA"
                     /locus_tag="ID725_RS20185"
                     /old_locus_tag="pgaptmp_004037"
     CDS             8722..10050
                     /gene="dsdA"
                     /locus_tag="ID725_RS20185"
                     /old_locus_tag="pgaptmp_004037"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGV7852525.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(10158..11696)
                     /gene="emrY"
                     /locus_tag="ID725_RS20190"
                     /old_locus_tag="pgaptmp_004038"
     CDS             complement(10158..11696)
                     /gene="emrY"
                     /locus_tag="ID725_RS20190"
                     /old_locus_tag="pgaptmp_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGV7852526.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(11696..12859)
                     /gene="emrK"
                     /locus_tag="ID725_RS20195"
                     /old_locus_tag="pgaptmp_004039"
     CDS             complement(11696..12859)
                     /gene="emrK"
                     /locus_tag="ID725_RS20195"
                     /old_locus_tag="pgaptmp_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGV7852527.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3828 a         3064 c         2841 g         3425 t
ORIGIN      
        1 taattcaata atgtctgtaa attgttatta cttataatta tacatttaac tatatctaca
       61 ttggctgctt ccaattacta cttacgtaac ggaatctcgg atcctctgtt tttggctcgg
      121 tataataaga atcatcaata gtaaagttcg acgctaattt atcccgaagc ccacctaaac
      181 gttcaatgct tgttagttcg ttagtaacat ccttgaaaac ggtagaaaat gctttatgtt
      241 gtttagactt cataaattca ggagtgctca gtgcaaactc tttatcaggc aaacctggaa
      301 tacccacgag tcctgtcaat gaatttatac attttatcaa aaaactgcct tgaatacctt
      361 gatttgatgg aacaccgtag cttcgtttaa tatctccaga tattttatct aaatcactta
      421 gtaattttgt tactttttta tttcctgcac ttatccccga ttcatcgtag gtcacatgaa
      481 tctctcggat tacatctcga cacgtacgaa aatgatcatg tgttctaagc tctaaagctg
      541 aattgataac ttgagagaca tttcctgttt cgtttgctag ccatgcagaa aatattggta
      601 aatctaatga aatagtagat gtgcgtccat gatctacaat ttcagatctt gcagtactga
      661 ttttatcaga caacgattgc accaaccttg cagtataatc atgaccaaaa gcacctgttt
      721 ttttcatcca atgcaattga tatgcatgcc ttattggatg aataaatgag tctgctttaa
      781 aatgcttagc aatgacgctg taataaatag atttatatgc caaccaattt agcgatgcga
      841 taaacatttc aagtgaacga gtcatacctc ctttaccgcg agtagatttt tccctttcta
      901 gttcaaattc acgctcagta tattcatgtc cagatgaact cactagcttc acatcggttg
      961 accagtaccc tttcgtatca gaagcatctg ataattcatt aaaaatcgat gcacttagtt
     1021 cactatattt cgcatattca ggagtaccgg gctgaccaag cattttcatt gttaagtaat
     1081 acacgctaga ccgaagatcc catgtgcaaa tcatattcag cttaagcatc tctacaagtt
     1141 gcctaaaatc atcgtcaaca aactccccac ctctaatttc ggaagtaatt tgccgagatt
     1201 caacttgtgc taactgatca agttcactga gttgaaaatc actttcagat acaaatttaa
     1261 taaagtcaaa tttagcaatg cgtttatctc tatattcttt tttataatta tctacacaga
     1321 tcagggtatc gtaaaaaagt atcgcttgaa taagattttc aaaagcgact aaatctccgt
     1381 taatagtatc tggattctta acaagaatat cacccagtgt tctttcgact gctgttaacg
     1441 aagcattatc aatcaacgca taagacatta aactacctcc attagataat atctaataac
     1501 attatatttc aattaggttt aaaaattgtg atacttatga cagatttgag tgacatttta
     1561 tcaatctact gattttgatg gtgaaattta ttagaaggta tgcgtagtgc tacgggagaa
     1621 atgagaaacg cgcccagcaa tggcgcatcc tttcttaagc aagttggcta gtatctatag
     1681 catccatcca atcaggagca attcccaaac gttccgcgaa ctcttcattg gtcacgtctt
     1741 ccattgagag actagccaga caatcgttaa tttcagaata gacgggggaa agtaataggt
     1801 attcccgcaa ctctttcagg tctttttcca tcttaacact ccttgttaat agaccgtcga
     1861 acaatggtac agatttgtac cattccaagt actggcaagc aaccccacca gcacaacaaa
     1921 ttatctcata cggtaaaaac ggtgtcgggc tgtttctttc ggctagctca atatatcacc
     1981 ttaaacacca gccgttatca ttgtctcaat tgggtatacc gtaatgacaa ggtgatacaa
     2041 agtctcaata gggttgtata ggaacatttt gactacacaa actgcattat gtatcatttg
     2101 cacttatatt cacaaccctt ttgagacaat gcaatgaacg ttagaattta ctgccgagca
     2161 tcaactgagg ggcaacacgc tgatcgagca cttaccagcc tacgtgaatt ttcaaaatca
     2221 aaaggctggc agattgcagg agagtatata gaaaatgcta gcggggcgaa gcttgagcgc
     2281 gtagagctta tgcggctatt gtctgaagcc cagtccggcg atctgttgct agtggaagct
     2341 atagatcgtc tgagtcgcct agaacatagt gcatgggtcg aactgaaaga tacgctcaac
     2401 cgaaaagggt taatcatcgt atcaatggat ttaccaacga gctggcagat ggtcgagatg
     2461 gcaggcaatg atctaaccag cggcatcctg agagccgtaa atgccatgtt gattgatatc
     2521 cttgccacaa tggcacggca ggattacgaa acacgccgta aacgccaaca gcaaggcatt
     2581 gaacgagcta aaagcgaagg catatacatt gggagggcga aaaatcagga agcccgagag
     2641 atcgttagag agatgttgga gcaaggagta aaacctgaac tcatcatgaa ggcagcagga
     2701 atcagccgag cgacttacta ccggataaaa aacgagctat tgatcgtaaa atcagaataa
     2761 aatttgaaag gtcgtcaggc ttgtttcatc aggtaaaaac cagcatttct gatcacaacg
     2821 cttgggggca cctattgggg cactcaccaa aatcgaaaga gaaataaact acaattcaaa
     2881 ttgttagcta atttttcgat tcctgcaggg acaccagata cccttcaaac caaatctacc
     2941 ttcatcccgt aaacgatggg tttggcagca cacttgccct atatctactc atttttactg
     3001 caacaggttg aaatctcagc actgtcagaa agcgctgatg actaaacagc ccagggccgg
     3061 gcgatgtaac catcacacag aatcctgata gcgaaatacg gcgtgactcg atacttcact
     3121 ccgcaatgca ttccttgatg aattcgcagg cccgtgatac acgggacagg tcgcagaatg
     3181 acgacaatgt cctggaaatc agcgaaccgt gtatccggag tacatttgag cgactgtacc
     3241 agaacatgaa tgaggtgttt ggattaggcg attattagca gggctaagca ttttagtatt
     3301 attattttcc ggttgaagga tatggagata tcgacaacaa ccggaaaaag tttacgtcta
     3361 tattgctgaa ggtacaggcg tttccataac tatttgctcg cgttttttac tcaggaagaa
     3421 aatgccaaat agcaacatca ggcagacaat acccgaaatt gcgaagaaaa ctgtctggta
     3481 gcctgcgtgg tcaaagagta tcccagtcgg cgttgaaagc agcacaatcc caagcgaact
     3541 ggcaatttga aaaccaatca gaaagatcgt cgacgacagg cgcttatcaa agtttgccac
     3601 gctgtatttg aagacggata tgacacaaag tggaacctca atggcatgta acagcttcac
     3661 taatgaaata atccaggggt taacgaacag cgcgcaggaa aggatacgca acgccataat
     3721 cacaacaccg ataagtaatg cattttttgg ccctacccga ttcacaaaga acggaataat
     3781 cgccatgcac agcgcttcga gtaccacctg gaatgagttg agataaccat acaggcgcgt
     3841 tcctacatcg tgtgattcga ataaacctgc ataaaagaca ggaaaaagtt gttgatcaaa
     3901 aatgttatag aaagaccacg tcccaacaat aaatatgacg aaaacccaga agtttcgatc
     3961 cttgaaaact gcgataaaat cctctttttt tacccctccc gcatccgccg ctacgcactg
     4021 gtgatcctta tctttaaaac acatgttgat catcataaat acagcgccaa atagcgagac
     4081 caaccagaag ttgatatggg gactgatact aaaaaatatg ccggcaaaga acgcgccaat
     4141 agcatagcca aaagatcccc aggcgcgcgc tgttccatat tcgaaatgaa aatttcgcgc
     4201 cattttttcg gtgaagctgt caagcaaacc gcatcccgcc agatacccca ggccaaaaaa
     4261 gagcgccccc agaattagac ctacagaaaa attgctttgc agtaacggtt cataaacgta
     4321 aatcataaac ggtccggtca agaccaggat gaaactcata caccagatga gcggtttctt
     4381 cagaccgagt ttatcctgaa cgatgccgta gaacatcata aatagaatgc tggtaaactg
     4441 gttgaccgaa taaagtgtac ctaattccgt ccctgtcaac cctagatgtc ctttcagcca
     4501 aatagcgtat aacgaccacc acagcgacca ggaaataaaa aagagaaatg agtaactgga
     4561 tgcaaaacga tagtacgcat ttctgaatgg aatattcagt gccataatta cctgcctgtc
     4621 gttaaaaaat tcacgtccta tttagagata agagcgactt cgccgtttac ttctcactat
     4681 tccagttctt gtcgacatgg cagcgctgtc attgcccctt tcgccgttac tgcaagcgct
     4741 ccgcaacgtt gagcgagatc gataattcgt cgcatttctc tctcatctgt agataatccc
     4801 gtagaggaca gacctgtgag taacccggca acgaacgcat ctcccgcccc cgtgctatcg
     4861 acacaatcca cagacattcc agcaaaatgg tgaacttgtc ctcgataaca gaccaccacc
     4921 ccttctgcac ctttagtcac caacagcatg gcgatctcat actcttttgc cagggcgcat
     4981 atatcccgat cgttctgtgt ttttccactg ataagtcgcc attcttcttc cgagagcttg
     5041 acgacatccg ccagttgtag cgcctgccgc aaacacaagc ggagcaaatg ctcgtcttgc
     5101 catagatctt cacgaatatt gggatcgaag ctgacaaaac ctccggcatg ccggatctcc
     5161 gtcatcgcag taaatgcgct ggtacgcgaa ggctcggcag acaacgcaat tgaacagaga
     5221 tgtaaccatt cgccatgtcg ccagcagggc aagtctgtcg tctctaaaaa aagatcggca
     5281 ctggggcgga ccataaacgt aaatgaacgt tccccttgat cgttcagatc gacaagcacc
     5341 gtggatgtcc ggtgccattc atcttgcttc agatacgtga tatcgactcc ctcagttagc
     5401 agcgttcttt gcattaatgc accaaaagga tcatccccca cccgacctat aaacccactt
     5461 gttccgccta atctggcgat tcccaccgca acgttagctg gcgcgccgcc aggacaaggc
     5521 agtagccgcc cgtctgattc tggcaagaga tctacgaccg catcccctaa aacccatact
     5581 ttggctgaca tttttttccc ttaaattcat ctgacttacg catagtgata aacctctttt
     5641 tcgcaaaatc gtcatggatt tactaaaaca tgcatattcg atcacaaaat gtcatagtta
     5701 acgttaacat ttgtgatatt catcgcattt atgaaagtaa gggactttat ttttataaaa
     5761 gttaacgtta acaattcacc aaatttgctt aaccaggatg ataaaaatga cgcaatctcg
     5821 attgcatgcg gcgcaaaacg cactagcaaa acttcacgag cgccgaggta acactttcta
     5881 tccccatttt cacctcgcgc ctcctgccgg gtggatgaac gatccaaacg gcctgatctg
     5941 gtttaacgat cgttatcacg cgttttatca acatcaccca atgagtgaac actgggggcc
     6001 aatgcactgg ggacatgcca ccagcgacga tatgatccac tggcagcatg agcctattgc
     6061 gctagcgcca ggagacgaga atgacaaaga tgggtgtttt tcaggtagtg ctgtcgatga
     6121 caatggtgtc ctctcactta tctacaccgg acacgtctgg ctcgatggtg caggtaatga
     6181 cgatgcaatt cgcgaagtac aatgtctggc taccagtcgg gatggtattc atttcgagaa
     6241 acagggtgtg atcctcactc caccagaagg aatcatgcac ttccgcgatc ctaaagtgtg
     6301 gcgtgaagcc gacacatggt ggatggtagt cggggcgaaa gacccaggca acactgggca
     6361 gatcctgctt tatcgcggca gttcattacg tgaatggact ttcgatcgcg tactggccca
     6421 cgctgatgcg ggtgaaagct atatgtggga atgtccggac tttttcagcc ttggcgatca
     6481 gcattatctg atgttttccc cgcagggaat gaatgccgag ggatacagtt accgaaatcg
     6541 ctttcaaagt ggcgtaatac ccggaatgtg gtcgccagga cgactttttg cacaatccgg
     6601 gcattttact gaacttgata acgggcatga cttttatgca ccacaaagct ttgtagcgaa
     6661 ggatggtcgg cgtattgtta tcggctggat ggatatgtgg gaatcgccaa tgccctcaaa
     6721 acgtgaaggc tgggcaggct gcatgacgct ggcgcgcgag ctatcagaga gcaatggcaa
     6781 acttctacaa cgcccggtac acgaagctga gtcgttacgc cagcagcatc aatctatctc
     6841 tccccgcaca atcagcaata aatatgtttt gcaggaaaac gcgcaagcag ttgagattca
     6901 gttgcagtgg gcgctgaaga acagtgatgc cgaacattac ggattacagc tcggcactgg
     6961 aatgcggctg tatattgata accaatctga gcgacttgtt ttgtggcggt attacccaca
     7021 cgagaattta gacggctacc gtagtattcc cctcccgcag cgtgacacgc tcgccctaag
     7081 gatatttatc gatacatcat ccgtggaagt atttattaac gacggggaag cggtgatgag
     7141 tagtcgaatc tatccgcagc cagaagaacg ggaactgtcg ctttatgcct cccacggagt
     7201 ggctgtggtg caacatggag cactctggca actgggttaa cataatatca ggtggaacaa
     7261 cggatcaaca gcgggcaagg gatccgcgtc acacttcccc cttcacgacc ttcaataata
     7321 tgcaatgcag cttcccgccc gataatgtca tgtggaagct gaattgtggt cagcggcggt
     7381 aaaaacagat gcccgacgcc aaccagatta tcaaagccca ttacggcgac atcctgcggg
     7441 atacgtaccc ccttagccag gagaacctga taagccacaa aggctgcgcg atcgttacca
     7501 catatcagaa catcaaaatc tggtttgccc gatttgaagt ggtcattgag taaacttgcg
     7561 agatcggtgt agtgatcatc acctgttgcc atgtgaaatt gtttcacctc agccagatct
     7621 cgtccagcat cacgccaggc ctgctcaaat ccctgccgac gataccctgt tgccaacgca
     7681 ctttccggta gccagaagca taacggttga cgatagcccg ccgcgagcaa atgctgtgtt
     7741 gattcatatt gtgcagtgta atcatcaggg atataactgg gtaacgctgg gtcatccgcc
     7801 acacagttcg ccaatacaat attttcacca tacagagact caggtagcgt gatatgtcgc
     7861 agccccattg tagtatagat aatgccatcc ggacggtggg caagcagctg acgtgccgcg
     7921 cgggcagcgt catcttcaga aaaaatattg attaaaaaac tattccagcc gaactcgctg
     7981 gcggtttgct caatggcaag cagaatatca acagagaaag gagtggtagc agtgtcctgc
     8041 gccagcacgg cgagagtcga cggcttacgt ccttgagcgc gcatcttacg ggcggaaaga
     8101 tcaggaacat aattcagggt ctggattgcc tgcaatacgc ggtcacgcgt tgcaggacgc
     8161 acagattctg cattatgcat cacccgggag actgtcatca tcgacactcc cgccaggcgt
     8221 gcgacatcct ttaatgaagc catacccaag ccgtttgccg taaaacgggc actgtagcag
     8281 aaacagacgt cactggcgag atccaacgaa attctcgacg ccctatcacc cgacacggca
     8341 atacaataaa aaataacaat aattgccgga taattgtacc caattccgcc tctgttctcg
     8401 ccaacgagtg accatatagg caagtaagaa aaacatgcca actggcatcg ccaacctgga
     8461 gaagaaaata taaatatgga tcattgtgct gccgctgtat cccgacatca gcccggaaat
     8521 tattgcgatt gctatcggtt caggcgcgat tggctgcacg atcgttacgg actcgctttt
     8581 ctggctggtg aagcaatatt gcggcgctac gctcaatgaa acatttaaat actatacgac
     8641 agcgacattt atcgcttcag tcgtcgctct ggcgggcaca ttcctgctgt catttatcat
     8701 ctaagcgcaa agagacgtac tatggaaaac gctaaaatga actcgctcat cgcccagtat
     8761 ccgttggtaa aggatctggt tgctcttaaa gaaaccacct ggtttaatcc tggcacgacc
     8821 tcattggctg aaggtttacc ttatgttggc ctgaccgaac aggatgttca ggacgcccat
     8881 gcgcgcttat cccgttttgc accctatctg gcaaaagcat ttcctgaaac tgctgccact
     8941 ggggggatta ttgaatcaga actggttgcc attccggcta tgcaaaaacg gctggaaaag
     9001 gaatatcaac aaccgatcag cgggcaactg ttactgaaaa aagatagcca tttgcccatt
     9061 tccggctcca taaaagcacg cggcgggatt tatgaagtcc tggcacatgc agaaaaactg
     9121 gctctggaag cggggttgct gacgcttgaa gatgactaca gcaaactgct ttctccggag
     9181 tttaaacagt tctttagcca gtacagcatt gctgtgggct caaccggaaa tctggggtta
     9241 tcaatcggca ttatgagcgc ccgcattggc tttaaggtga cagtgcatat gtctgctgat
     9301 gcccgggcat ggaaaaaagc gaaactgcgc agccatggcg ttacggtcgt ggaatatgag
     9361 caagattatg gtgttgccgt cgaggaagga cgtaaagcag cgcagtctga cccgaactgt
     9421 ttctttattg atgacgagaa ttcccgcacg ttgttccttg ggtattccgt cgcaggccag
     9481 cgtcttaaag cgcaatttgc cgagcaaggt cgtattgtcg atgctgataa ccctctgttt
     9541 gtctatctgc cgtgtggtgt tggcggtggt cctggtggcg tcgcattcgg acttaagctg
     9601 gcgtttggcg atcatgttca ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg
     9661 ttaggcgtcc atacaggatt acacgatcag atttctgttc aggatattgg tatcgacaac
     9721 cttaccgcag cggatggcct tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg
     9781 gagcgtctgc tggatggctt ctataccctt agcgatcaaa ccatgtatga catgcttggc
     9841 tggctggcgc aggaagaagg tattcgtctt gaaccttcgg cactggcggg tatggccgga
     9901 cctcagcgcg tgtgtgcatc agtaagttac caacagatgc acggtttcag cgcagaacaa
     9961 ctgcgtaatg ccactcatct ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag
    10021 atggagcaat atctggcaaa aggccgttaa taacgtttca acgcagcatc gcaatccttt
    10081 ccctgggtga gcgatgctgc cgatggcgca gacttaagat ccccagtctt acccgctata
    10141 accccctgct tttaccatca cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa
    10201 accaaaaccg taagtaagat aaaagcaata gccgccatac ggaaaatctc atttgcagaa
    10261 atagaaagtg actgctgggt tatttcatta ttaatttcat taagaactcc tgaaagcgaa
    10321 ccatagtatt tatccataat ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg
    10381 gtttctgtca actgactatg gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac
    10441 gacgtaccaa ctgatcctga caaggtacga aaaaaattac tcatactcga ggcattggca
    10501 aatttattat ctggcaagcc tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca
    10561 acggcgaatc cctgaaaaaa ctgcggcaaa atgatacctg taaaatcaat cgttggcata
    10621 aatgtcacag aacgccagta atagcaaacc gcatacatca aaaaactgaa tgtcaccaac
    10681 acccgcatgt ctattttgtt gccataacgt cctatcaaag gtgaaattaa tagtggcatg
    10741 atgccgatgg gcgcataagc aagtccggcc catatcgcat tataccccat cgtttcctgg
    10801 agtaactgcg gcataaggac gatcgctcca gagtaaaata aatacgcgca tgtgatactc
    10861 acaataccaa tggtgaagtt acgggactta aacaaactga gatcaagaat cgggttctct
    10921 gaggtcgact cccaaatgac taaagagatc agaaaaataa ctgatactac tgttaatatt
    10981 attattgtac tcgagttgaa ccaatccaga tcgcgccctt tatcaagcat aatttgcaag
    11041 ccaccaacac cgagcactaa cagggtcagt cctggtagat tcattttgac cggtgaagtc
    11101 tcagtttctc ttcctttaag taaggttaag cataatgtca ggacgataat ccccataggg
    11161 acattgatta aaaatatcca accccagcta aagttatcac aaatataacc gcccaatatc
    11221 ggcccacata tcggagcgat aatcacggtc attgaccata atgccagagc aaatgttctt
    11281 ttttctggtg gataattcct taataataaa ctctgtgaca gtggaattaa cggccctgcc
    11341 attaacccct gaacgactct aaaaaatatc agcacatcaa gattgatcga taaactacac
    11401 attaaggaag acagcgaaaa aaaagtgact gaaagtaaaa ataaccttaa ttcgcctatt
    11461 ctttgtgcca gcctgccggt aacagggatc gcaatggcat ttgctacgcc aaacgaggtg
    11521 ataacccagg tgccttcgtc tgttgatgct cccagaaagc cagatattgt cggtattgcg
    11581 acgttagaaa tagtggaatc caacatttgc ataaacgtcg ctaatgacaa tgcaatagtg
    11641 acgcaccata acgtcccacc ggttaatggt gccggagttg atttagtgat tgccattaaa
    11701 gttgtccatt atgcgaaata atgttgctaa tttctttttc tatcggactg gtatcgataa
    11761 ctaaagcctt actggtataa gccggcatgg acgtcacggt tgaagccagc tcaggcatct
    11821 cagcaatgtc ttcgttcttc gtatcaatag ttgcagtcat cgataaacca atgcgcaagg
    11881 ggtgttccat gagttctttt ggatcaagag aaacttcaac cggtacacgc tgaacgattt
    11941 tgatccagtt ccctgtcgca ttttgtgcag gtaataagga gaacgcattg ccggttccca
    12001 tattgatccc tgtcacccgg ccatgaaaca caacattctc accataaaga tcgctgataa
    12061 tattgaccga ttgaccaatc cgtacatccg tgagttgtgt ttctttaaag ttagcattaa
    12121 cccacatttg acgtgccggt acgacagcca ttaacgattg tccgggactc actgtttcgc
    12181 cgacctgaac acttctctgg gcaatatagc cggtaaccgg actcttaata tccgtacgtt
    12241 taagcgccaa ccaggcttct ttggttgcat ccgcagcttc aacgacttgt ggctgacggt
    12301 ttaatggtgt gttcattact aaagctttat tcgctttata agcctggata gcggcattca
    12361 atgccgcttt gctacttatt aacgtatctt tggtatgctc cagcgtttct tttgaaataa
    12421 ccccctgctt cgctaaaggc acacgacggt tataatcttc taaagattgt tgatactgaa
    12481 tacgtgctga agcgacttcg gcactgtatt gtttatcctg taagtacagt ttattcgttt
    12541 gccgaacaat atttgccaga ttatttttag ctttattgag tgcgatagtg gcatcagttt
    12601 tatccagtga aactaaaatg tcaccctgtc gaacgtagtt cgtatcttta tgattaacga
    12661 cagtgacact acctgagact tgtgcagaaa ttggatctgc attccccgtg acataggcgt
    12721 catctgtact aatcatgtct tctaattcca ttgaccaata ggcataagca cctgaaaacg
    12781 caataaataa aactatcgcc aataaagaaa agtattttct tctgttagaa tgttttttat
    12841 ttgaattaat ctgttccact attatctctc atttcgcata gatgaaattt atgaattgaa
    12901 tgaaaagagt attcattctt catatcaaac acagtgcatg tgttgcacat tatccctgga
    12961 acgttggagg cctaatttaa atgaggtgca aaaaaaacaa cagaggacta aaccgtggct
    13021 tttgcaatac aattcttacg cctgtaggat tagtgagaag acttatagtg ctaacttgaa
    13081 actataaatc atcggtataa tccctgattt tattgttgac atttcatata tgccgactat
    13141 ttatatggta tacttgtc
//
LOCUS       DABSYT020000116        13112 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.116, whole genome shotgun sequence.
ACCESSION   DABSYT020000116 DABSYT020000000
VERSION     DABSYT020000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.116"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            169..405
                     /gene="lpp"
                     /locus_tag="ID725_RS20200"
                     /old_locus_tag="pgaptmp_004040"
     CDS             169..405
                     /gene="lpp"
                     /locus_tag="ID725_RS20200"
                     /old_locus_tag="pgaptmp_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGV7852528.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(468..1472)
                     /gene="ldtE"
                     /locus_tag="ID725_RS20205"
                     /old_locus_tag="pgaptmp_004041"
     CDS             complement(468..1472)
                     /gene="ldtE"
                     /locus_tag="ID725_RS20205"
                     /old_locus_tag="pgaptmp_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGV7852529.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(1621..2037)
                     /gene="sufE"
                     /locus_tag="ID725_RS20210"
                     /old_locus_tag="pgaptmp_004042"
     CDS             complement(1621..2037)
                     /gene="sufE"
                     /locus_tag="ID725_RS20210"
                     /old_locus_tag="pgaptmp_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGV7852530.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(2050..3270)
                     /gene="sufS"
                     /locus_tag="ID725_RS20215"
                     /old_locus_tag="pgaptmp_004043"
     CDS             complement(2050..3270)
                     /gene="sufS"
                     /locus_tag="ID725_RS20215"
                     /old_locus_tag="pgaptmp_004043"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGV7852531.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(3267..4538)
                     /gene="sufD"
                     /locus_tag="ID725_RS20220"
                     /old_locus_tag="pgaptmp_004044"
     CDS             complement(3267..4538)
                     /gene="sufD"
                     /locus_tag="ID725_RS20220"
                     /old_locus_tag="pgaptmp_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGV7852532.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            complement(4513..5259)
                     /gene="sufC"
                     /locus_tag="ID725_RS20225"
                     /old_locus_tag="pgaptmp_004045"
     CDS             complement(4513..5259)
                     /gene="sufC"
                     /locus_tag="ID725_RS20225"
                     /old_locus_tag="pgaptmp_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGV7852533.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(5269..6756)
                     /gene="sufB"
                     /locus_tag="ID725_RS20230"
                     /old_locus_tag="pgaptmp_004046"
     CDS             complement(5269..6756)
                     /gene="sufB"
                     /locus_tag="ID725_RS20230"
                     /old_locus_tag="pgaptmp_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGV7852534.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVVSDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(6765..7133)
                     /gene="sufA"
                     /locus_tag="ID725_RS20235"
                     /old_locus_tag="pgaptmp_004047"
     CDS             complement(6765..7133)
                     /gene="sufA"
                     /locus_tag="ID725_RS20235"
                     /old_locus_tag="pgaptmp_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGV7852535.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(7682..7870)
                     /locus_tag="ID725_RS20240"
                     /old_locus_tag="pgaptmp_004048"
     CDS             complement(7682..7870)
                     /locus_tag="ID725_RS20240"
                     /old_locus_tag="pgaptmp_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGV7852536.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(7970..8380)
                     /gene="menI"
                     /locus_tag="ID725_RS20245"
                     /old_locus_tag="pgaptmp_004049"
     CDS             complement(7970..8380)
                     /gene="menI"
                     /locus_tag="ID725_RS20245"
                     /old_locus_tag="pgaptmp_004049"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGV7852537.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            complement(8377..11433)
                     /locus_tag="ID725_RS20250"
                     /old_locus_tag="pgaptmp_004050"
     CDS             complement(8377..11433)
                     /locus_tag="ID725_RS20250"
                     /old_locus_tag="pgaptmp_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGV7852538.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            11822..12934
                     /gene="ydiK"
                     /locus_tag="ID725_RS20255"
                     /old_locus_tag="pgaptmp_004051"
     CDS             11822..12934
                     /gene="ydiK"
                     /locus_tag="ID725_RS20255"
                     /old_locus_tag="pgaptmp_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGV7852539.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
BASE COUNT         3062 a         3522 c         3241 g         3287 t
ORIGIN      
        1 tcctgttttc tctcaatttt tttatctaaa acccagcgtt cgatgcttct ttgagcgaac
       61 gatcaaaaat aagtgccttc ccatcaaaaa aatattctca acataaaaaa ctttgtgtaa
      121 tacttgtaac gctacatgga gattaactca atctagaggg tattaataat gaaagctact
      181 aaactggtac tgggcgcggt aatcctgggt tctactctgc tggcaggttg ctccagcaac
      241 gctaaaatcg atcagctgtc ttctgacgtt cagactctga acgctaaagt tgaccagctg
      301 agcaacgacg tgaacgcaat gcgttccgac gttcaggctg ctaaagatga cgcagctcgt
      361 gctaaccagc gtctggacaa catggctact aaataccgca agtaatagta cctgtgaagt
      421 gaaaaatggc gcacattgtg cgccattttt ttgtctgcta tttaccacta ctgcgtcgcg
      481 cgtaacatat tcccttgctc tggttcacca ttctgcgctg actctactga aggcgcattg
      541 ctggctgtgg gagttgctcc actgctcacc gcaaccggat accctgcccg acgatacaac
      601 gctttatcga ctaacttctg atctacagcc ttattgtctt taaattgcgt aaagcctgct
      661 ggcagtgtgt atggcattgt ctgaacgttc tgctgttctt ctgccgatag tggtcgatgt
      721 acttcaacat aacgcatccc gttaggctcc acggaatatt tcactggttc gttgatcact
      781 ttcaccggcg ttcccgtccg cacgctggag aacaaggctt taatatccgg tgcattcatg
      841 cgaatacacc ctgaactgac gcgcaaaccg acgctgtccg gcgcactggt gccatgaatg
      901 aggtattcgc cattaccatg cgcgaggcgc agtgcgtaac gtcctagcgg gttatttggt
      961 ccggcaggaa cgactggcgg taatttaatg ccacgctcca gcgaacgttg acgaatgcct
     1021 gccgtaggcg tccaggttgg gttagggatt ttctgcccca cacgcgtttc catcactggc
     1081 gtttccagcc cctgcaagcc aatacctatc ggataaacct gcacgatatt ttctcccggc
     1141 ggataataat aaaggcgcag ctctgcaagg ttaacgataa tcccctgacg cggcgcatca
     1201 ggtaataaca gttgcgaagg aatagttatc gtcgtaccgg gttttggcac cggggcgata
     1261 gtgttattgg cttcaaggat caacattgcc gcagtatcaa aacgtcgggc gatggcctga
     1321 aggtttttat ccccttcctg caccgtatac gtttgatttt gcccaaccag tcggcttccg
     1381 gttggtggta gcggataatc aaccgcccag gcagcctgga tggcactaaa agcgccgata
     1441 agcgtgagtg taagcaaaga cgcgcgtttc attgtaaacc tcctgtattt gccggagact
     1501 cacgctgaaa cgtcggatgg cgcttatgtt cacctgaaac cgaaacactc ctgtgcaggt
     1561 cagtgtaaac attgaccatc cggcaatgtg agccaaccgg atgaaagctg tccctttagt
     1621 ttagctaagt gcagcggctt tggcgcgaat tgcgcgaatc atcgcttcca gaccttgtga
     1681 acgagatggg gtgagatgtt gggtgagtgc cattttttca aaccacggac gcacatcgaa
     1741 attgacaaca tcctgcggcg tcatctgatc gtagagaata aagacgaccg caataagccc
     1801 tttcacaatc gccgcatcgc tgtcgccctg caattcaata attccctgcg cattctgacg
     1861 catgacaatc cacacctgac tctggcagcc ctgaatgcta ttttgtgggc ttctgtcttc
     1921 gtcgcgtaat tctggcagac gctggcccag ctcaataatg tagagatatt tctcttccca
     1981 gttggcgcag cgtaaaaaat tacgcagcaa cttttcttta tccggcaata aagccatagt
     2041 gcctccctgt tatcccagca gacggtgaat acgttgcagt ccggtcacca gacgatccac
     2101 ttcttcatgg gtgttataca tggccagcga cgcccgacac atagcaggga cgttataata
     2161 ggccattaat ggcatagcgc agtgatgtcc ggtacgcaca gcaatgccgt aattatcgag
     2221 aaaactgcca acatcatagg cgtggtgttt accgagatta aaagcaataa cgccaagcct
     2281 gttttgtggg ccatagagag tgagatccgg tacagattcc agctgtgata gcgcgtaatg
     2341 catcagattc tgttcatact cggctatgtt attaagcccc tgcgccgaaa catactccag
     2401 cgccgcgcca agaccaatga tgcccccggt attgggtgta ccggcttcaa accgccacgg
     2461 tgctttggtc caggtagtgc cttcactcag gctgacggtg gcgatcatag aaccgccccc
     2521 ttcccacggc ggcatctcct gtagcaaggc ttctttgaca taaagaatgc caatcccggt
     2581 ggggccatac agtttatgcc cggaaaacac gtaaaaatcg caatccagcg cctgaacatc
     2641 caccggatga tgcatcaccg cctgagcgcc atccaccagc acttttgcgc catgctggtg
     2701 cgcaagcgtg atcatttccg ccagtggatt ttctgtgcca agcacgttgg agacatgagt
     2761 aattgccagc aggcgagttt tctcatcaaa cagcgtaggc agcgtctcca gttgcaacgt
     2821 accgtcggga ttgagcggga tcacacgcag ctctgcgcca acgcgtgcgc aaagcatctg
     2881 ccagggaaca atgttagcgt ggtgctccat ctgacttatg atgatgttat cgcccgcccg
     2941 cacgttgctg ttgccccagc tattggcgac cagattgatc ccttccgtcg tgccgcggac
     3001 gaacaccagc tcttccgccg aacgggcatt aataaacagc gatgcccgct tgcgcacgtt
     3061 ctccattttc tcggtcgcct gggcgcttaa ggtatgaata ccgcgatgca ccgccgcgta
     3121 gccatgacga taaaactcgg cctcggcgtc aatcacctgg ctcggtttct gcgcactggc
     3181 ggcgctgtcg agataagcca gcggcaaacc gtttacctca cgcgaaagca ccggaaagtc
     3241 ggcccgcact ttgtcgacgg aaaaagtcat cttgcacctc ctggcagccg ttgaccgatt
     3301 cgggtcagca cctgctgttt aagcccctca tcacgcagtg cttccgtcag ttcggcagca
     3361 aaggcgtaaa tgatcatctg ctgggcatcc tgctgattga tcccgcgcga gcgcagatag
     3421 aacatctgtt catcatcaat acgccccacc gtcgcgccgt ggctgcattt cacatcatct
     3481 gcatagattt ccagctgcgg tttcgtatcc acttccgcca gtttgcccat cagcagatta
     3541 ttgttggtca tctgaccatc cgttttgatg gcgtgctgcg cgacgttgat caaaccgtta
     3601 aataccgcgc ggcctttgtc gctgacgata gttttgtgca actgtcggct gttacaaaaa
     3661 cctttattgt gttccagcca ggtacgggta tcacacacct cgtttttcac cggcatcgcc
     3721 aggctattga tccgcagcgt gcagttttcg ccattgagtt gcgtactggt gttgtgtcgt
     3781 aacactgcgc cacccagcag gaaactgtgg ctaaatgcgg tggcatcgtc agccagcaac
     3841 aaatcgttat gggcaaagtg gtgactgagc gggttttcaa acgccagctt gatatgctgc
     3901 aagtgggcat tcgctgcgac gttgatagag aaccgtgccc cggtaaaatg acgagcatca
     3961 ttaaggctga caaaatgttc gatcaccgtt gcttcggcac cttccgccag ctccagatga
     4021 tgtcggtaat gggcagtgtt cacctcttca cctgccacgc cctgggtgat atgcattaac
     4081 agcaatggct ttgccggccg ttgaccgcgc ttcacggcga tatgcgtcac gctttgtgcc
     4141 aggctttccg tcaaatgcag aaacacttcc gcctgaatag catcgggtaa cccctgacgg
     4201 tcgtcgttaa tgctcacttc atacccgctg ccttcagtcg catcgctcag cgcaggcacg
     4261 taacgtccat cgacaaatac cagccgcaca gcgtctaacg ttaacgctaa ggcatctcgc
     4321 tgctgtgggg atatctctcc cgcaatgctg acaaactgac tattggtcag cccttccagc
     4381 ggcgtatatt tccagttttc atgtttacgt gtcggcagtc cggtacgcag caattgttgt
     4441 aaatgctgct gtgcttgcgg ggagcgtttt gtcccttcag cttcaaacaa gtgatgccac
     4501 tgttgcagcg cgttactgct gttcggtaag ccagccataa ccctgctcct ccagttgttt
     4561 gaccaacgtg aaatcgccgg atttcacaat tcgtccctga tatagcacat gaacgtaatc
     4621 aggcttgatg tagtcgagaa tgcgttggta gtgcgtaaca atgatgaatg agcgcttgcc
     4681 atcacgcagc gagttcacgc catcggcgac cacttttaat gcgtcgatat ccagcccgga
     4741 gtccgattca tcaagaatgc ataactccgg ttccagcacc gccatttgca aaatatcgtt
     4801 gcgctttttc tcgccgccgg aaaaaccaac gtttaccgaa cgggttaata aatcttccgg
     4861 catcttcagg agagcgattt tctcttccat caaatcctga aaatcaaagc ggtcgagcgt
     4921 ttcctggccg cgatagctgc gcaccgcatt aagtgccgtt tgcaggaaaa actggttact
     4981 gacacctgga atctccaccg gatactggaa ggccataaag atgccttcgc ctgcacgatc
     5041 ttccggcgac agtgcaagca aatctttgcc tttgaactca accgtgccgc ccgtcacttc
     5101 ataatcttct cgcccggcaa gcgttgccga taaggtactt ttgcccgaac cgtttggccc
     5161 cataatggcg tgaacttcgc cgggacgaac gtcgaggctt aatccacgca ggatagcttt
     5221 atcttccacg ctgacgtgta aatctttaat acttaacatg tttattcctt atccgacgct
     5281 gtgttcaaga ctgatggcga ggagtttttg tgcttcaacg gcaaattcca gcggcagttc
     5341 tgagaacacg tctttgcaga aaccgttaac aatcatcgag atggcgtctt cttcgctgat
     5401 cccgcgttgc aggcagtaaa acagttgatc ttcaccaata cgtgatgtcg ttgcctcgtg
     5461 ctccagttgt gcgctgttat tgcgacattc gacatacggg aaggtatgcg ccccacaatt
     5521 agcgccaatc agcattgagt cgcactgagt gaaattgcgc gcattggttg ccgtcggcat
     5581 gattttcact aagccgcgat aactgttctg gctatgtccg gcagagatcc ctttcgagat
     5641 aatggtcgat ttggtgtttt taccgatgtg gatcatcttg gtgccggtat ccgcttgctg
     5701 atgaccgctg gtcagcgcca ctgagtaaaa ctcaccaatg gagttatcgc cgcgcaaaat
     5761 gcagctggga tatttccacg taatcgctga cccggtttct gattgcgtcc atgacatttt
     5821 gctgttttcg ccttcgcaca aagcacgctt ggtgacgaag ttgagaatac cgccggtgtt
     5881 gttatcgcca gggaaccagt tttgtaccgt ggaatatttc acctcggcgt ttttatggat
     5941 gatgacttcc accactgccg cgtgcaactg atagctgtca cgcaccggag cggaacagcc
     6001 ttcaatgtag ctgacgtagc tgtcttcgtc ggccaccaga atggtgcgct caaactgtcc
     6061 ggttttttcc gcgttaatgc gaaaataggt ggaaagttcc atcgggcagc gcacgccttt
     6121 aggcacataa ataaacgtac cgtcagagac taccgccgca ttaagcgcgg caaagaagtt
     6181 gtcattcccc ggcaccacgg tgccgagata tttacgcacc agttccgggt gatcgtggat
     6241 cgcctcacca aaggaacaga aaataattcc ctgctccgcc agtttttcgc gataagtagt
     6301 ggcaaccgaa actgagtcga aaatggcatc caccgccacc tctttgcctt cccgcacggg
     6361 aacacccaac tgctcaaacg ccgcctccac ctctttactt aaaaaggcgt tcgcgccagt
     6421 ttgctgcacc gcgccaggtt cagacgcgca agtgtcgtca caattaccgc acgatggtgc
     6481 cgagtagtag ctgtaatcct gataattcag cttgtcgtag tgcgctttca accagtgcgg
     6541 ttcttccatc tccagccatg cgcgataggc gtttagacga aactccagca tccactccgg
     6601 ctcattacgc ttcgccgaaa ttgcgcgcac cacctcttcg tttatcccct ttgccagctc
     6661 atcggtggct aactgggtga agaatccttc tttataattc agcgggccgc cggtccaggt
     6721 tttgacatcg tcagttgctt cagtattacg agacatagta ccgcctatac cccaaagctt
     6781 tcgccacagc cacattcatt ctgggcttta gggttgtgaa atttgaatat ctgattaagt
     6841 ccttcacgaa cgaaatcgac ttccgtgcca tcaataaacg gcatcgcttg cagcgggaca
     6901 aacagcttcg cgccgtcgtg ttcaaacagc agatcgtctt tgtccggctc gctaacactg
     6961 tcgagcacat agccaaagcc cgcgcagccc gtttgcttca cacctaagcg cacgccgacc
     7021 atacccggct gctttgccac cagctcgcgg atgtggacag cggctgcggg tgtcagcgtt
     7081 aagccttgcc aggcgaaatc ctgtggatta aaggttcctg aatgcatgtc catcgattta
     7141 cctcacttca tcgctttcag cgtataacag catgttagtg ataatgatta tcagttcaac
     7201 ccagcaaagg caggggcttt accgtaaaac atgctttttg cctgctttta ataagcatag
     7261 accctgatgt gtgggttaac aggcacgcta agaattaggt atctcattgt tagataatga
     7321 ttattatcta aggagcatta aggctgtgat gaaaaagaaa aagttgtatt gaaaatgact
     7381 atttaagaga taggtaaaaa agtgcagcgt tcagaaataa gaaaaccctt aagtctgtgc
     7441 gacacaggct taagggtttc taccccatcc ggcgcttatc tccggcactc tcagtggctt
     7501 agctcttgaa ggggcgataa gaataatctc ataaagctaa gccgccgttt taacacaaac
     7561 tgcgattagt attatttttg aacaatatca ggcgggtaga taagcagtat taagaaggtc
     7621 atcgaacctg gacggaggtt aatccaggtc gatttggcga acttgcggca ttaagtcagg
     7681 atcaatgcac gcccagacgc caggcaaagt agatttcttc ttttaattca gcagaagaga
     7741 gagtaagcag gtcagcaaat tcaagttcta gttgtttcag acgtttaaga tattgggcag
     7801 gtgaaagatt gctctggtca cggcgtaaaa attcaatggc cagctgggtg ggatcaagtt
     7861 gagtagacat agcatcctcg cttttagaca agacctgcac agtataccac cgtttactgt
     7921 gcagataatg accaaaagca atatgcgtca cacttttctg gtgacaacgt cacaaaatgg
     7981 cggtcgtcaa tcgtgacgaa cagcacaaac gccctttctc atcggagatt tcaatctgcc
     8041 agacctggtg acgcgaaccg agatgcaacg gtttgcatac gccgcgcacc cgcccttctc
     8101 gtgccgagcg gacgtggtta gcattgattt ccagaccaac cactttttgc tcaccttcgg
     8161 tacataaata accggcaacg gaaccgatac tttcggccag taccacggat gcacctccat
     8221 gtagcaaccc gaaaggctgc tttgtccgcg agtctactgg cattgtcgct tcaagggtgt
     8281 catcaccaat atgttcaaag cgaatatcca gtaaccccac catgtttcct tcacccatgg
     8341 cattcagtgc ttccaggggg atttttcgtt tccatatcat ttaataatct ccagtaaagc
     8401 ctgcacagga tggcgtaccc ccgtgccttc aacccgtttg acctgactac ggcaggaata
     8461 tccggtcgcc agacagcggt tacgcggcag tcgctgcata gcctgatgcc aggataactc
     8521 atagatcccg agcgaatttt tatggttttt cgcttcatgt ccgtaagtcc ctgccatgcc
     8581 gcagcaaccc acgctgacat tttccagttt cgcgccaaaa cgggcaaata tcgcggccca
     8641 ttgtgctggc gcacccggca aggcggtaac ttcggtacag tgaccaaaga aataccatga
     8701 ttcaccgctg actgtagcca ctggctgtga ctcaagtgcg cttgccagcc attcattcgc
     8761 cagtaagacg ttaaactcgc cacgctcctc gcccagcgcc agtttatatt catcgcgata
     8821 acaaagtacc agcgccggat cgacgcccac cattggcata cccagcttcg ccatacggtt
     8881 gaggaaatcc gccgtctttt tcgccgtctt cgcaaaacga ttaagaaaac ctttaatatg
     8941 ctgggctttg ccatttggcg aaaatggcag taacacaggc tggaaaccta atttttcgac
     9001 cagacggaca aaatccgcca ccacttgcgc atcgtaatag ctggtaaagg ggtcctgcac
     9061 caccaacact gtgcgcgctt tctgctctgc attgagagat tcaagctgtt ccagcgtcat
     9121 gtttgccgag cgatgcccca ccatttgttg ttgtagcgag gggaccgaca gcagcggcaa
     9181 atcaaccatg ccgatatgtt tttccgagag tttgcgcacc agcggctggt taatgaagaa
     9241 gttaaaggtt ttcggcgcgc gtgccatcag cggcgcgtag ctctcgaccg tagcgacgag
     9301 gtggtcgcgc agcgggcgta aataacgggt gtgatagagc tgcagaaaac gagagcgaaa
     9361 ctccggcaca tcaattttga tggggcactg ggtcgaacaa gctttacagg ccagacagcc
     9421 cgacatcgcc tctttgactt cgtgtgagaa gtcatattcg cctttattcg catgccagct
     9481 attgcgcgtg cgggcaatta acgtccgcaa gctgacgccg gattcaggca gttcttgttc
     9541 cagtttgagt ggatcaacgc cgcgatccgc caacaaacgc agccattcac gcaccagcgt
     9601 tgcgcgccct ttcggtgaat gaatccggtt ctgggtgatc ttcatcgacg gacacatcgg
     9661 actacgggca tcaaagttga agcataaacc gttgccgtta cactccatcg caccgcgcca
     9721 ctgctggcgt accgcaatgg ggatctgccg atcgaatgta ccgcgcttca ccgcgtccac
     9781 tttcatcatc ggcgcatcga gaccttctgg cgggcaaatc ttccctgggt tgagtcggtt
     9841 atgcgggtca aatgccgctt tcactttgcg cagttctgca aaaagttcct caccgaaaaa
     9901 cgccgggctg tattcagcgc gaaaaccttt gccgtgctcg ccccacaaca aaccaccgta
     9961 tttcgcagtc agcgccacca cgtcatcaga gatttgcttc atcaaaatct cttgttgcgg
    10021 atcgcacata tccagtgccg gacggacatg caagacacct gcgtcgacgt gaccgaacat
    10081 accgtagctt aagccgtggc tgtcgagcag cgcgcgaaat tcagcaatat aatcggccag
    10141 atgttccggc ggtacgcagg tatcctcagc aaacggaatt ggcttagcgg cacctttggc
    10201 attgccaagc agaccaacgg cttttttgcg cattgcatag atacgttcaa cccccgccag
    10261 ctcacggcac atctgccagc cgatgacacc tgcttgatga ctggcgatca gctcatccaa
    10321 ccgcgcacag agtgcattta cccgctcgtc aatcagcgcc tcatcatcac cagcaaattc
    10381 cacaatgttc agcccgagca tctctttgtc aggcacatcg gtaatcaact cgctgacgga
    10441 atgccagaca atatcttccc gcgccagatt cagcactttt gagtccaccg tctctaccga
    10501 aagcgcccgc gcctcaacca taaacggcgc gttacgcagc gcagagtcaa aagagtcata
    10561 tttgacattc accagacggc gcactttagg caagcgcgta atatccagcc gcgcttcggt
    10621 aataaaggcc agcgtccctt ctgaacccgt cagaatgcgc gtcagatcga actcggtcat
    10681 ctcatcgtta aagacatgac gcagatcgta accggtaaga aagcggttaa gtttggggaa
    10741 gttgtcgatg attaactggc gttgctgacg gcaacgttga taaaccgtgt tataaattcg
    10801 cccgattgtg gtattggatt tacccagcgt ttccgccagc tcgacgggta aaggttgcgt
    10861 atcgagaata tcgcccccca acaataccgc acgtaggcca agtacgtgat ctgacgtttt
    10921 gccatagacc agcgatccct gaccggatgc atcggtattg atcatcccgc cgagcgttgc
    10981 ccggttgctg gtcgaaagtt ctggtgcaaa aaagtagccg aacggtttca ggtactgatt
    11041 gagttggtct tttatcaccc cggcctcaac gcgcacccag ccctcttcag ggttaatttc
    11101 gatgatgcgg ttcatatggc gggacatatc aacaataata ccctggttga gcgcctgacc
    11161 gttagtgccg gtgccgccgc cgcggggggt aaagatcagc gatgaatagc gttcctgcgc
    11221 ggcaagacgg gcgatcagcg ccacatctgc ggttgaacgc ggaaatacca ctgcatcggg
    11281 gagaagttgg taaatactgt tgtcggtcga cattgtcaga cgatcagcat aacttgtcgc
    11341 cgtatcgccg gtaaaacctt gttgctccag ctcttgcaaa aaattaagca ccagttgaac
    11401 gacgccgggt gcctgggaaa tctgtggaat cattatattg accctttcct gcggtctgtg
    11461 atgtaggtcg atacactgtt ctttcaggcg gctgcaatag cgcacagaaa ggtgatgttt
    11521 atttactcta tggatttcga gttgcaggaa ggcggcaagc gagtgaactc caggagctta
    11581 caacagtaag taactggggt gaacgaacgt agccgcagca tatgcaactt gaaatacgac
    11641 gagtaaatcg tttgcgtgtt gcctgagttg ttgtaccaca tttttttcta acacgcccat
    11701 cagaattaag ggcagaatcg gcctgttaaa aaccgctgaa attgctcatc attatgcagg
    11761 tgagtttcgc gtgttcacgt cgcgtcgacg atttgacgca caaaaaaggt gaaaagtagt
    11821 tatggtaaat gttcgtcagc ccagggatgt cgcacaaatt ctgctttcgg tgctgttttt
    11881 agccatcatg attgtggcat gtctgtggat tgttcaaccc tttattctcg gctttgcatg
    11941 ggccggtacg gtggttatcg ccacctggcc ggtattgtta cgtttgcaaa agatcatgtt
    12001 tggccgccgc tctctcgccg ttctggtgat gacgctgtta ttagtgatgg tgtttatcat
    12061 ccctattgct ttgctggtta acagtatcgt cgacggcagc ggcccgctaa ttaaagccat
    12121 ttccagcggt gacatgacgt tacccgatct ggcgtggctt aataccattc cggtgattgg
    12181 cgcgaagctg tatgcaggct ggcacaactt gctggatatg ggggggacgg cgatcatggc
    12241 gaaagtccgc ccttatattg gcaccaccac tacctggttc gttgggcaag cggcgcatat
    12301 cgggcgcttt atggtgcatt gtgcgctgat gcttctcttc agtgccctgc tgtactggcg
    12361 cggtgaacag gtggcacaag gcattcgcca ttttgcgacc cgtctggcag gcgttcgcgg
    12421 agatgccgcc gtactgctgg cggcacaggc tatccgcgcg gtggcgctgg gtgtggtggt
    12481 gacggcgtta gtacaggcag tgcttggcgg tatcggcctc gccgtatccg gcgtacctta
    12541 tgcaactttg ctaacggtgt taatgatcct ttcctgcctt gtccagcttg gcccgttgcc
    12601 ggtactgatt ccggcgatta tctggctcta ctggactggc gataccacct ggggaacggt
    12661 attgttagtg tggagcggtg tggttggcac gctggataac gtcatccgcc caatgttaat
    12721 tcgcatgggt gccgatttac cgctgatcct gatcctctct ggcgttattg gtggtttgat
    12781 tgctttcggg atgatcggtc tgtttattgg tccggttctg ttagccgttt cctggcgtct
    12841 gtttgccgcg tgggtggaag aggtcccacc gccgacggat caaccggaag aaattctcga
    12901 agaacttggc gaaatccaga aaccgaataa gtaatttctc atcaggcggc tctgccgcct
    12961 gattgttaac caccgctaat tgatatttgc ttgctcttcc ccatattttc ctgcttaccg
    13021 ccattcagct ggtagtacct gtcgcaaatt ctttacagtt tttaaactaa tgagacgaat
    13081 ctgatcgacg caaaaagtcc gtatgcctac ta
//
LOCUS       DABSYT020000117        12811 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.117, whole genome shotgun sequence.
ACCESSION   DABSYT020000117 DABSYT020000000
VERSION     DABSYT020000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.117"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..820)
                     /locus_tag="ID725_RS20260"
                     /old_locus_tag="pgaptmp_004052"
     CDS             complement(<1..820)
                     /locus_tag="ID725_RS20260"
                     /old_locus_tag="pgaptmp_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7852540.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVTNTEAITLKIDPSVVLATRKYVDDKVLELR
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPAAGNNTTRIATTEFVQTAITA
                     LINGAPDTLDTLKEIAAAINNDPKFSTTINNALSGKQPLDETLTHLSGKDVAGLL"
     gene            complement(817..1428)
                     /locus_tag="ID725_RS20265"
                     /old_locus_tag="pgaptmp_004053"
     CDS             complement(817..1428)
                     /locus_tag="ID725_RS20265"
                     /old_locus_tag="pgaptmp_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HGV7852541.1"
                     /translation="MSDSRLLPTGSSPLEVAAARACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWSFSVDRWDDKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFPASAIHLIDNLRVNA"
     gene            complement(1421..2329)
                     /locus_tag="ID725_RS20270"
                     /old_locus_tag="pgaptmp_004054"
     CDS             complement(1421..2329)
                     /locus_tag="ID725_RS20270"
                     /old_locus_tag="pgaptmp_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HGV7852542.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYSVVTGGSDE"
     gene            complement(2334..2681)
                     /locus_tag="ID725_RS20275"
                     /old_locus_tag="pgaptmp_004055"
     CDS             complement(2334..2681)
                     /locus_tag="ID725_RS20275"
                     /old_locus_tag="pgaptmp_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HGV7852543.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLALMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(2678..3313)
                     /locus_tag="ID725_RS20280"
                     /old_locus_tag="pgaptmp_004056"
     CDS             complement(2678..3313)
                     /locus_tag="ID725_RS20280"
                     /old_locus_tag="pgaptmp_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGV7852544.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTCRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(3380..3832)
                     /locus_tag="ID725_RS20285"
                     /old_locus_tag="pgaptmp_004057"
     CDS             complement(3380..3832)
                     /locus_tag="ID725_RS20285"
                     /old_locus_tag="pgaptmp_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGV7852545.1"
                     /translation="MNEFKRFEDRLTGLTESLSPSGRRRLSAELAKRLRQSQQRRVMT
                     QKAPDGTPYAPRQQQSVRKKTGRVKRKMFAKLITSRFLHIRASQEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(3825..4292)
                     /locus_tag="ID725_RS20290"
                     /old_locus_tag="pgaptmp_004058"
     CDS             complement(3825..4292)
                     /locus_tag="ID725_RS20290"
                     /old_locus_tag="pgaptmp_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7852546.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVVGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(4255..4500)
                     /gene="lysC"
                     /locus_tag="ID725_RS20295"
                     /old_locus_tag="pgaptmp_004059"
     CDS             complement(4255..4500)
                     /gene="lysC"
                     /locus_tag="ID725_RS20295"
                     /old_locus_tag="pgaptmp_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HGV7852547.1"
                     /translation="MLCAGCTSAPPAQTPVIVANACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(4400..4825)
                     /gene="lysB"
                     /locus_tag="ID725_RS20300"
                     /old_locus_tag="pgaptmp_004060"
     CDS             complement(4400..4825)
                     /gene="lysB"
                     /locus_tag="ID725_RS20300"
                     /old_locus_tag="pgaptmp_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HGV7852548.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLRASLDRANNVASGQQ
                     TTIIMLKNQLHVALTRADKNEMAQVALRQELENAAKREAQREKAITRLLNENEDFRRW
                     YGTDLPDAVRRLHQRPACADASDCRQRLPESEPLPDAGQ"
     gene            complement(4813..5238)
                     /locus_tag="ID725_RS20305"
                     /old_locus_tag="pgaptmp_004061"
     CDS             complement(4813..5238)
                     /locus_tag="ID725_RS20305"
                     /old_locus_tag="pgaptmp_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HGV7852549.1"
                     /translation="MKKLSLSLMLHVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRFEGREPCQS"
     gene            complement(5253..5750)
                     /locus_tag="ID725_RS20310"
                     /old_locus_tag="pgaptmp_004062"
     CDS             complement(5253..5750)
                     /locus_tag="ID725_RS20310"
                     /old_locus_tag="pgaptmp_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HGV7852550.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(5750..6031)
                     /locus_tag="ID725_RS20315"
                     /old_locus_tag="pgaptmp_004063"
     CDS             complement(5750..6031)
                     /locus_tag="ID725_RS20315"
                     /old_locus_tag="pgaptmp_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGV7852551.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(6035..6238)
                     /locus_tag="ID725_RS20320"
                     /old_locus_tag="pgaptmp_004064"
     CDS             complement(6035..6238)
                     /locus_tag="ID725_RS20320"
                     /old_locus_tag="pgaptmp_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HGV7852552.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAMLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(6238..6747)
                     /locus_tag="ID725_RS20325"
                     /old_locus_tag="pgaptmp_004065"
     CDS             complement(6238..6747)
                     /locus_tag="ID725_RS20325"
                     /old_locus_tag="pgaptmp_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGV7852553.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(6847..7590)
                     /locus_tag="ID725_RS20330"
                     /old_locus_tag="pgaptmp_004066"
     CDS             complement(6847..7590)
                     /locus_tag="ID725_RS20330"
                     /old_locus_tag="pgaptmp_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HGV7852554.1"
                     /translation="MTSPAQRHMMRVSAAMTAQRDAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLALTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(7594..8667)
                     /locus_tag="ID725_RS20335"
                     /old_locus_tag="pgaptmp_004067"
     CDS             complement(7594..8667)
                     /locus_tag="ID725_RS20335"
                     /old_locus_tag="pgaptmp_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HGV7852555.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDLIMAGFNGVRRAETSDRSS
                     NQMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNREQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPTKVTAEPGA"
     gene            complement(8726..9580)
                     /locus_tag="ID725_RS20340"
                     /old_locus_tag="pgaptmp_004068"
     CDS             complement(8726..9580)
                     /locus_tag="ID725_RS20340"
                     /old_locus_tag="pgaptmp_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HGV7852556.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFSRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            9754..11526
                     /locus_tag="ID725_RS20345"
                     /old_locus_tag="pgaptmp_004069"
     CDS             9754..11526
                     /locus_tag="ID725_RS20345"
                     /old_locus_tag="pgaptmp_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HGV7852557.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNSAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFLLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            11526..12560
                     /locus_tag="ID725_RS20350"
                     /old_locus_tag="pgaptmp_004070"
     CDS             11526..12560
                     /locus_tag="ID725_RS20350"
                     /old_locus_tag="pgaptmp_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGV7852558.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
BASE COUNT         2729 a         3757 c         3497 g         2828 t
ORIGIN      
        1 cgagaagacc ggcaacatcc tttccactca aatgagtcag cgtctcatcc agtggctgct
       61 tacctgacag cgcattgtta atggtggtgc tgaatttcgg gtcattgtta atggccgcgg
      121 caatttcttt cagtgtgtcc agcgtgtctg gcgcgccgtt aatcagagcg gtaatagcgg
      181 tctgaacaaa ctcagtggtc gcaatccgcg tggtgttatt tcctgcggca ggcgttggcg
      241 cttttggttc tccggtaaat gtcggattat gtttctgcgc atactgggta tgaggatcct
      301 gtgcggcaat gtggtttctc atctggtcat ccacatacag ccttaattcc aggactttat
      361 catccacgta tttacgggtc gccagtacca ccgacgggtc gattttcagc gtgatggctt
      421 cggtattcgt gacaaccaga atcatgcgga tagtctgggt acgaccgctg ccttcctgta
      481 actgcggttt gtacgtttcc gggcagttcg ccaccgcaat gagtacgcct tcatcatcat
      541 aaagcccaat ctcacggatc cagaatccgc cctcgttctc agggatgatt tgctccgcaa
      601 taatctggct ctggttgttc gggtcaacac tcagaagatt cagcggcgcg atgcgtttct
      661 ggttaataag ttttgtctgt gccgggtcag gtgtcggcaa gacaccattt gcatcaccaa
      721 cggccatttg cgtcagattc agcttactgc cgagcatcgt cgcgttagcc agccgtgccg
      781 cgccctgatt agtcagaatg gcgtagtatt tcactgtcat gcgtttactc tcaggttatc
      841 aattaaatga atggccgagg ccgggaaata atcccctccg acaataatgg cctccggggt
      901 gtagggataa accgtcaggg cgtcgccgtg atagcatccc gcaccggcaa aaatgttgcc
      961 ggttgtactt aaactgatag ccagtcccgt cagatggcgg cttgcaggtt ttgcatcagc
     1021 aacgagacgc tccagctcct gatacatttc ctcggtaata ccctgctcaa gcacgccaac
     1081 aacgatgcgg aacgtccccg gctcctcgtt gagctgccac cactccctca cctcaatcag
     1141 atagccgagc ggctccacca cacggcgaat cgcaccaata gtgcccttat ggcagtgaat
     1201 gaaatacgca tcgcggataa cagcgcgttt tgtcgcttcc ggccacttat catcccagcg
     1261 gtcaaccgaa aatgaccacg ccagccacgg cagcagattt gccggacagg tatccgggtt
     1321 ccacagctca cgaatactga ccggcgtttt ttcaatttcc gcacaggctc ttgcggcggc
     1381 gacttcaagc ggtgatgagc cggtcggcag caggcgcgaa tcactcatcc gagcctccgg
     1441 tcacgacgct gtattcggtg cagaaagacg cctgcgtact gttgagcaca atgtcagcca
     1501 gaggtgcagt cagttcgaca cgttgcacgc cttccacatg caaagcggca taaatggcag
     1561 acagacggat gtcgcgccca agccggtgct gtgccgtgat gtacgcttcc agttttttca
     1621 cggcagcagc gcggatgggt tcgctttcgg gacctgggta aaggtaaagc gtggcgttta
     1681 tctgatattc aacaatggcg gcagactgca cggtcacgcg gtcggccacc ggcctgacgt
     1741 cctcgccatt aagggcgtta cgcaccacgg ccagcaggtc ttcggatgcg acgccgttat
     1801 tttcacgtga cagcacggag atagtgacgc aggccggaga cggactggtg acagagatat
     1861 ccgcgacacg cccgtcagca ctgcgaccat gatactgata ggctcccacc gacccggcga
     1921 cgcttaagcc ctcaaacgcc tgctgaatac gcagacgata atcggtgtca gactccatca
     1981 ctgccggtgt cggcgggata gtcgaatcat ctgccggggt gataatcagg cgtgtggtgt
     2041 tgtaattggc accaatcaca tcaaggtcat taccggcggc acaggccagc atcaccgccc
     2101 gtgcggcctc attcacacgc tgacgccaga taagctcacg ataagcattt tcctccagaa
     2161 gtttgacgag aggctcggat tccagcgtca gggtacgggc gaccgcctcc tgctggtctt
     2221 ccgggtaaag ggaaatcagt gtcgccttgc gttcggcaag aatggtttca aagtccagct
     2281 cctcgaccac atccggtgcg ggtagctggt tcaggtcgat aatcggcatg gtttcaactc
     2341 acagggatgg ttaacgaaag tggctggccg gtgtcgttgt gctggccggt taacgtgacc
     2401 gtcattcgcc cgtcaaaact gcgctcagta gtgacggatg acagggtgac gcggggttcc
     2461 catttcagca ccgccatgta acaggcgacc ttaatctgca actcaagcgc cggggtctgc
     2521 ggctggtcaa tcattaatgc cagcaacgag ccgtaatcac gacgcatcac ccgtgagccg
     2581 accggtgtac gcaggatatc gccgatactc tggctgatat gctcaaggtc agtgacagtc
     2641 aggccatcac tgcgattcat tccgagataa cgcgctgtca taaaggactc ccggttgtgc
     2701 cgccgctgtc gccggggtgt ttatgggtat gcagtacctt accgtttgat gagagttcac
     2761 caccggtgtg ttcaatgttg ccgcgcatcg tcccgccctt ctgtacttcc agcgtgccgg
     2821 taatcagcct gttggtgcag accacctccg gtgtgtccag ggtgatgcgg gttgacgctt
     2881 tcaccatgac caccggcacc gtggcagtaa cagaatcaga agccgtcacg ctggccgttt
     2941 taattccgct taccgtcagt gcactggttt caggttcata ctcaatcacc gccccgtcag
     3001 ggaaacggat atgcagggca tccgccgacg cagacggcgc ggggttatcg ccggaataaa
     3061 tccccggcag aacaaacgct gtgtcaagtt caccgcccac ggccagaatc agcacctgct
     3121 cccccacgga aggtgcccac catgtgcgcg aacgcccggc acgacaagtc agccactgaa
     3181 gccagtcggt gcacatgccg ccggtctgca cacggcagcg accggcgtta aggtcggttt
     3241 cgacgacaag gccggtgcga atcatgttgc gcagtgcgcg cgcgagttcc tggatatttg
     3301 cgagagtgtt cataacggga aggatgccgc cgggtcatac cggcggcaat gtgacgatga
     3361 ggtgtcagga atggcacaac taacggtcga ggtgagccag gataatctct tcaatcatct
     3421 gcacatcctc accggtaaag ccgagcagag gacgcgccgg ataatcaatt ttcttaccgt
     3481 ctttccggtt ttcttccgac agaccgaact gatgcacgct ggcgattttc ggtgacttcc
     3541 cgccgtaaaa ctccattgat gcctgttcct ggctggcacg gatatgcaaa aaacgactgg
     3601 taataagttt cgcaaacatt tttcgcttaa cgcgaccggt cttttttctg acgctctgct
     3661 gctggcgtgg cgcgtagggt gtgccgtccg gggctttctg tgtcatcacc cgacgctgct
     3721 gactctgccg cagacgtttc gccagttcgg cgctcagtcg tcgacgccct gacggtgaca
     3781 gcgattcagt cagtccggtc agccggtctt caaaacgctt aaactcattc atcccacttg
     3841 ctcaccagtt cgccattgat ataaagctcc atcgggcggg tgaccggctc cggcggcgga
     3901 ggttccggga tattcttcac atgcagtgcg ccgaccacct cactgaccag cgtgcgctcg
     3961 gtcagcatca ggctgatgct gatatcaaag ctgctgtcat tgttgatgtc tgcataaaac
     4021 gtgaagccct ttttctggcc tgcgtcggtg gtcatgatgt cgggctgatt ttcccgcagc
     4081 cacgccagca ccggcacgat gagcaggtca aaatcaccgg taaagtcggt cacaatgaca
     4141 ttgagcgtgt aacgcttttc gaatgacagc gacgccgcca gtgtggaggc aatactcccg
     4201 ttatccacga atatccgcag catatcgggg ttagttttca gcaccgtgac ggcatcagtc
     4261 agcgccctgc gcaggctgtc gggtttgagc atcgttttcg tcctgacagt gtttaatcat
     4321 ttttacctgg ctggcacagc gtgccagcgc gttctcaagc tgccggatat cagcacttaa
     4381 atcgccgttc gtctgcgggt cactgcccgg catcgggcaa aggctcactt tcgggcaggc
     4441 gttggcgaca atcactggcg tctgcgcagg cggggcgctg gtgcaaccgg cgcacagcat
     4501 caggcaggtc agtgccgtac cagcggcgaa aatcttcgtt ttcattgagt aacctcgtga
     4561 tggctttctc gcgctgtgct tcacgcttcg cggcgttctc cagctcctga cgcagtgcca
     4621 cctgcgccat ctcgtttttg tctgccctgg tgagggcaac atgaagctga tttttcagca
     4681 tgatgatggt cgtctgctgc ccactggcga cattgtttgc cctgtccagc gaggcgcgca
     4741 ggctggcgtt ttcatgcttc gccagaaaca gaccggccac cgccagtgat aacaacacaa
     4801 ccagcacaat catcagcttt gacatggttc ccgcccctca aaacgctgac ggcaggccgt
     4861 acgtatcagc cggaaaaaca gcgacgccac aagataaatc agcgcggtaa aaatccaccc
     4921 ggcggcgacc agcgagataa acgtcgccac catcaccacc agagccgccg cccgtctgcg
     4981 ccacggcacc ggctgcaaaa acagcgacgt gacaatcttc acggccagcg attccggcgg
     5041 cagctcccgc ccgtagcgtt ccagtacata ctctgtggca tacaccccga caccaccggc
     5101 aaccacacag ataaccgtcg ccagaatcgc ccaggcagcg acaaaactga cggccacgct
     5161 ctgcggataa atcagggaca gtgccagcat cagcgccagc gacacgtgca gcatcagtga
     5221 aagggataat ttcttcatgg tgtttactcc gtttaagccg gtacgccgcc agcggtacgc
     5281 cagacggtga ccagtttttc cagtgaatgc tcacgctgac cgtaaccggc acccggcagg
     5341 gacgcccaga tattgcgaca gcgtgaaatg gcacgctcaa tgcgtcccgc ccggatgtca
     5401 tccagtgcac cgcgttcgcg gatcaactga atggcgagcc tgtcctgtga caacggactg
     5461 aaatccggca gggcaagctg tttgcggtaa tgcggccaga acaggtaaag ctgctgatag
     5521 cgaccggagg ccgtggattt ttcaccgcga cggttaaaca ccttcgccgg tcggccatgc
     5581 gcgaacgggt ggtcactgta gtcggtgaaa atttccggct ttccgtccag tccggtgact
     5641 atcacgtcat agccccggtt tttcgtcagc ggatgattcg ccgtcccttc ggacacggcc
     5701 agcatgtcga gaaaggcggc gatattctga tgcgtgttaa ttaccggcat tacggtttcc
     5761 ccctgccctt aaagcggcgc tgaatggcaa tctcaatcac ctgataaccg gcgataccca
     5821 gcatggagcc gataccgcac accgcaggca gtgacaggtc aggaaactgc accagaacaa
     5881 caccggcaac catcgagaca aaaccaccga gcaacatgcg cccgataaac agacgcgggg
     5941 tgatgggttc accaccggca agcaccttgc cgacaacaat cagcactcca atcatgaaaa
     6001 gcgacaggac gcttttttct tctgctgtca tgcgttactc ccacagattg acagtttcag
     6061 ccacgggcgc ggtctgaacg tcgggcagtt cgacggcggt gccgtgcggc agcatcgcac
     6121 ccagttcagc cagtcccgga tttgcggcga gcacggtctc aaccacgccc tcagtgcgcc
     6181 cgtaataccg gacacaaatg gcgtcgagcg tgtcgccctg tagcgcaaag gtcttcatca
     6241 gatttgactc acgatgcagc gcggcttgtc ctggatacgt gccaccgccc agcgcatatc
     6301 ccgccacagt tcatcaatgg tgctgtcaat gctgtcggcc ttcttgtcgc ccttggcact
     6361 ggcatccacg ccgcgataac gctcataaag cgatgcggtc gccatcgcac acacggcgcg
     6421 ctcgtagtaa aaaactttga tgctttcacc gtcgatgtcg tccgccggga cgtccgccag
     6481 acgcgtaaaa ccggcagcaa ttttctgttc gcggtactcg tacagctccg cattcgtttc
     6541 cgccatgcct gacttgatgg cctcacgcag acgggcgggg gcgacggtct gctcaaggcg
     6601 catacgttcc cggacgcgct tcgggtcgat atcgggaaaa aagaacgtgt ttttaatcac
     6661 cggctcgtcg cctgccggtt gcgggatgac caccgtaccc tcaccggaca cgggagcctc
     6721 ctttcgcgga ataatcagcg tcatcatgac tacctctgaa aagtcgggcg gtggacgccg
     6781 gtgcagtgtc aggtgattca ccctcactga ccggcgtgcc gccctggcgc ggggcgcatt
     6841 cggttgttaa ctggctttct ttttcgggcg tccacgtttt gccggtgtca cgctccgggt
     6901 cttacgcggg gcgcgggtgg ccgctttggg ctgcggctcc ggcttcggtt tcagctcccg
     6961 ctccagtcgt tcaatctctt ttttgacgcc tgcctgacag tcgagctgtg tcgcacgttg
     7021 caggtgagcc agcgcaccgg cggcatcacc agcgtcacgc agaaacagac cggtgatttt
     7081 gtgcagcttt gcacgcactt catcaggcat gtctgccgtg gcggtcagtg caagggtctc
     7141 cgtcagcagg cgggtatcca cagactcacc ggcagcgtgg gcgcgcatgg ccgcgagcgc
     7201 cacctcctcg gtgaacatat acggcggggt gcggcggtgt ttacccggca tggtcagacc
     7261 gtacttcagg gcataacggg caatctccag cgcaccggca atatcgccgg tatccagacg
     7321 ccacagcatg accgtcatca gaatgtcatc ctgtgcacct ttgccctgct ccagcacgcc
     7381 gttcacccac ggcaaccaga acggcagcag ttcgcgtttt ttcgcggcct tcagctcttt
     7441 tgaataaatc gctttcagtg tgcgctggtc tgcggccagc ttgaccagca tctgctcata
     7501 gacagttgca tgtcgcagcg gggcggcgtc ccgctgcgcg gtcatcgctg ccgagacccg
     7561 catcatgtgg cgctgtgcgg gactcgtcat cggttacgct cccggctctg cggtcacttt
     7621 agtcggtgtg gagaagtcac cgaccttaat tttttccacc agacaaccgg cggcgtagtc
     7681 ttccaccacg taatcaatgt tcattgactc gtagttctcc acgcggtcga gtttcgggtt
     7741 ttccacaatc acgcggcgat ggctgtcatc catgtagtag atggacaggt tttccagctt
     7801 cgtgatgagc atcgcatccg ccgggaagta cgggacgcgt accgccggca ggttaccgat
     7861 gcgtttctgg ctgatgatga cgtcagcggc cagcatctcg ctgttgtcct gctccctgtt
     7921 gacgatggga aaatacttgt ccgccagcag ctgacgcccc acaatcacca caaggtcagg
     7981 gtcttcctga taccacggct caatcaggtt gttggtcgca tccatcacca gtgcatcgag
     8041 gctggcataa tcaccgccct tacccacgcg gatgacctca gaggtcgtgt gaccttcctc
     8101 gtcagtaacc ttgctcatca cgcgcgccgg ggcttcattg cggtatttct gcagccagcc
     8161 gaccgccaca tcctgcagca tctggttact gctgcggtca gaggtttcgg cacgcctcac
     8221 gccgttaaaa ccggccatga ttaaatcaag ggactggcgt ttgataatgg cgttacggac
     8281 acggagctgg aaatcctgat aacgcgccca caggtcaagc gttttgtagc ggatataaaa
     8341 atcgaagtta atctggtcgc attcgtactt gtttgacgcc agcttcgaga agtccttcgg
     8401 ctgacgctcg gtgccaccgg cggtgtctgt ggtgctggcg atggagccgg tgacaccaat
     8461 accaattttt tcccctttca tttcgctgac cggcacaatg ttgatgcggg tcagaaagtc
     8521 agaggactcc tgcatggtgt tcatcagggt ctgggtgacc gacggttcaa cggtgaattt
     8581 tttcgacaca tcaccggcgt cgatgccgtt cagttcggca acacgggaca ggtaggcatt
     8641 aaatttaaag cgggtttcct ggcgcatagt ttttcctgaa attaagggtt aatcgtgaag
     8701 gttttcccgg actggctgac gccggtcagc agttcgtcat cagggcgtca ccgccaccgc
     8761 cggtggcctt gctgcggcgc tgctgggtca gactttcggt gtggtcgaga ctgtttttca
     8821 ggcggctgaa tgcctggctg gtttcatccg ccctgtcagt cacctcctgc ttaagtgcgg
     8881 aaaaggcggt ttccatctca gcgagtcgct gctcagtggc gctcagcttt tcctgcacat
     8941 gttcagcaac agcggtcacc gcttcatgca cgtcattcag acgggcgtca tcgctggcct
     9001 gtttgcggcc aaaaatggat ttcacctttt cggtcagggc ggtgaacacg gtttcaggca
     9061 ggtcttcaaa ttccagctca acaggcgttg ctactgaaat caggttttca gggcttaatt
     9121 tgaagcggtt cagggggttg tgttttgccg tgcggcagaa ttccaggtat tccgtgccga
     9181 ggcttgccgg gtcatcggtg acggccagac ccaccagata acatttgccg gtgttggcaa
     9241 agttcggctg aatttccatt gaggtgtaga ccttctgcgc ggccttgttc atcgcgataa
     9301 ggtcatcggt cggggtgatt ttcgcaaaca gcgcccattt gcctttcagc gccgaatcgt
     9361 catcaatctt ttcggccttc agttcggcca catcgccata acgtttaaaa ataccgtcag
     9421 gcaggatgcc gcgcagatgt tccaggttaa tgcggcaacc atagacacgc gggtcaaagg
     9481 tttcggccat ttcctgaata tcctgcgcac tgatgacacg cccgtcacag gtgtcaccct
     9541 caacgccgat acgaaagaat tttgagactt tttttgccat tgtcaggagt cctgaatagt
     9601 gattagaaga gtcacatgtc ggcatcagtt tcccgacgat gcgcatcctc cgccatcagt
     9661 cccggatggc ttatcactga cacaacagca ccttagcgaa tcgcggggcg cgactcagta
     9721 gccttgccgt gtattcatca cggcgaggta ttcatgacca tcaccacaga caccactctt
     9781 ttacacgacc cgcgtcgtca ggcggcgctg ctgtactggc agggattttc cgtgccgcag
     9841 attgccgcca tgttgcagat gaaacgcccg acggtgcaga gctggaaaca gcgcgacggc
     9901 tgggacagcg ttgcccccat cagccgtgtc gaaatgagtc tggaagcgcg gctgacccag
     9961 ctcatcatca aaccgcagaa aaccggcggt gacttcaagg aaattgacct gctcggacgc
    10021 cagattgaac gactggcacg ggtaaaccgc tacagccaga ccggcaacga ggcagacctt
    10081 aatccgaaca tcgctaaccg caacaaaggc gggcggcgca aaccgaaaaa gaattttttc
    10141 agtgacgagg ctatcgaaaa gctggagcag attttctttg agcagtcttt cgaatatcag
    10201 ttgcactggt atcgcgccgg gcttgagcac cgcatccgcg atatcctgaa atcccgccag
    10261 attggcgcga cgttttattt ttcccgcgag gcgctgctgc gcgccctgaa aaccggtcat
    10321 aaccagattt ttctgtcggc cagtaaaacg caggcgtatg tgttccgcga atacatcatc
    10381 gcctttgccc ggctggttga cgttgacctg accggtgacc cgattgtcct gggcaataac
    10441 agcgcaaaac tgatttttct cggcaccaac tccaacaccg cgcagagcca taacggcgac
    10501 ctgtacgtcg acgagatttt ctggataccg aattttcagg tactgcgtaa ggtggcatca
    10561 ggtatggcct cacagagtca cctgcgctcg acctatttct ccaccccgtc cacgctggcg
    10621 cacgacgcct acccgttctg gtcgggtgaa ctgttcaacc ggggacgcgc cagcgccgcc
    10681 gaacgcgtgg aaatcgacgt cagtcataac gctcttgccg gtgggcttct ctgtgcggac
    10741 ggtcagtggc ggcagattgt caccattgag gacgccctga aaggtggctg cacgctgttc
    10801 gacattgagc agcttaaacg cgaaaacagc gccgacgatt ttaaaaacct gttcatgtgt
    10861 gaatttgttg acgacaaggc gtcggtgttc ccgttcgagg agctgcaacg ctgcatggtc
    10921 gacacgctgg aagaatggga agactatgcg ccgtttgccg cgaatccgtt cggctcccgc
    10981 ccggtctgga ttggttacga cccgtcacac cgtggcgaca gtgccggatg cgtggtgctg
    11041 gcaccgccgg tggtggccgg tggcaaattc agaatacttg agcgtcacca gtggaaaggc
    11101 atggactttg ccacccaggc tgaatccatc cgcaaactca ccgaaaaata caacgtcgaa
    11161 tacatcggaa ttgatgccac cggcctcggt gtcggcgtgt tcctgctcgt tcgctcgttc
    11221 tatcccgccg cacgcgatat ccgctacacg ccggaaatga aaaccgcaat ggtgctcaag
    11281 gcaaaagacg ttattcgccg tggctgtctg gaatatgacg tcagcgccac cgacatcacc
    11341 agctcgttta tggctatccg caagaccatg accagcagcg gacgcagcgc cacctatgag
    11401 gccagccgca gcgaggaagc cagccacgcc gacctcgcct gggcgaccat gcacgccctg
    11461 ttaaatgagc cactcaccgc cggtatcagc accccgctga catccaccat tctggagttt
    11521 tactgatgag caagaaaaaa gggaaaacac cgcaacctgc ggcaaaaaaa atgaccgcca
    11581 gcgccccgaa aatggaggca ttcacctttg gtgaaccggt gccggtactc gaccgccgtg
    11641 acattctgga ttacgtcgag tgcatcagta acggcagatg gtatgagcca ccggtcagct
    11701 ttaccggtct ggcaaaaagc ctgcgtgctg ccgtacatca cagctcaccg atttacgtca
    11761 aacgtaatat tctggcttca acgtttattc cgcacccgtg gctttcccag caggatttca
    11821 gccgctttgt gctggatttt ctggtgttcg gtaatgcgtt tctggaaaag cgttacagca
    11881 ccaccggtaa ggtcatcaga ctggaaacct caccggcaaa atatacccgc cgtggcgtgg
    11941 aggaggatgt ttactggtgg gtgccgtcct tcaacgagcc gacagccttc gcgcccggct
    12001 ccgtgtttca cctgctggag ccggatatta atcaggagct gtacggcctg ccggaatatc
    12061 tcagcgccct taattctgcc tggctgaatg agtcggccac gctgttccgc cgcaagtatt
    12121 acgaaaacgg cgctcatgcc ggatatatca tgtacgtcac cgatgccgtg caggatcgca
    12181 acgatatcga aatgcttcgc gaaaacatgg tcaagtcgaa aggccgcaac aactttaaaa
    12241 atctgtttct ctatgccccg caggggaaag ccgacggcat taaaattatc ccgctcagtg
    12301 aagtggcaac gaaggacgat ttttttaata tcaaaaaagc cagcgccgct gacctgctgg
    12361 acgcgcaccg catccccttt cagttgatgg gcggcaagcc ggagaacgtc gggtcgctgg
    12421 gtgatattga gaaagtggca aaggtctttg tccgcaatga gcttatcccg ctacaggaca
    12481 ggatccgcga gataaacggc tggctcggtc aggaggtcat ccgctttaaa aactactcac
    12541 tggacactga caacggctga acatcgccgc ctgcgggcgg cttttttaca ccccgtcatc
    12601 acgccctcac acgctcacca ccgcacaaaa caccccgcag acacaccaac gcctcaacgg
    12661 gcagactaag cgccatcacg acgcgctcag acgctgaaaa aataaaatca gcaccaccgc
    12721 cagcgcgcag tgctttcccc gcctcgcccg cccgcttcat gtggcgattt taatgcggtt
    12781 gcatttttat cgaaagtctc gtctcacaaa t
//
LOCUS       DABSYT020000118        12426 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.118, whole genome shotgun sequence.
ACCESSION   DABSYT020000118 DABSYT020000000
VERSION     DABSYT020000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.118"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(181..1539)
                     /gene="plaP"
                     /locus_tag="ID725_RS20355"
                     /old_locus_tag="pgaptmp_004071"
     CDS             complement(181..1539)
                     /gene="plaP"
                     /locus_tag="ID725_RS20355"
                     /old_locus_tag="pgaptmp_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGV7852559.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1529..1591)
                     /gene="yoeI"
                     /locus_tag="ID725_RS20360"
                     /old_locus_tag="pgaptmp_004072"
     CDS             complement(1529..1591)
                     /gene="yoeI"
                     /locus_tag="ID725_RS20360"
                     /old_locus_tag="pgaptmp_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGV7852560.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1806..2735)
                     /gene="yeeY"
                     /locus_tag="ID725_RS20365"
                     /old_locus_tag="pgaptmp_004073"
     CDS             complement(1806..2735)
                     /gene="yeeY"
                     /locus_tag="ID725_RS20365"
                     /old_locus_tag="pgaptmp_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGV7852561.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHNVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2781..3605)
                     /gene="yeeZ"
                     /locus_tag="ID725_RS20370"
                     /old_locus_tag="pgaptmp_004074"
     CDS             complement(2781..3605)
                     /gene="yeeZ"
                     /locus_tag="ID725_RS20370"
                     /old_locus_tag="pgaptmp_004074"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGV7852562.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            4031..4081
                     /gene="hisL"
                     /locus_tag="ID725_RS20375"
                     /old_locus_tag="pgaptmp_004075"
     CDS             4031..4081
                     /gene="hisL"
                     /locus_tag="ID725_RS20375"
                     /old_locus_tag="pgaptmp_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGV7852563.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    4057..4180
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4227..5126
                     /gene="hisG"
                     /locus_tag="ID725_RS20380"
                     /old_locus_tag="pgaptmp_004076"
     CDS             4227..5126
                     /gene="hisG"
                     /locus_tag="ID725_RS20380"
                     /old_locus_tag="pgaptmp_004076"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGV7852564.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5132..6436
                     /gene="hisD"
                     /locus_tag="ID725_RS20385"
                     /old_locus_tag="pgaptmp_004077"
     CDS             5132..6436
                     /gene="hisD"
                     /locus_tag="ID725_RS20385"
                     /old_locus_tag="pgaptmp_004077"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGV7852565.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6433..7503
                     /gene="hisC"
                     /locus_tag="ID725_RS20390"
                     /old_locus_tag="pgaptmp_004078"
     CDS             6433..7503
                     /gene="hisC"
                     /locus_tag="ID725_RS20390"
                     /old_locus_tag="pgaptmp_004078"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGV7852566.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7503..8570
                     /gene="hisB"
                     /locus_tag="ID725_RS20395"
                     /old_locus_tag="pgaptmp_004079"
     CDS             7503..8570
                     /gene="hisB"
                     /locus_tag="ID725_RS20395"
                     /old_locus_tag="pgaptmp_004079"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGV7852567.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8570..9160
                     /gene="hisH"
                     /locus_tag="ID725_RS20400"
                     /old_locus_tag="pgaptmp_004080"
     CDS             8570..9160
                     /gene="hisH"
                     /locus_tag="ID725_RS20400"
                     /old_locus_tag="pgaptmp_004080"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGV7852568.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9160..9897
                     /gene="hisA"
                     /locus_tag="ID725_RS20405"
                     /old_locus_tag="pgaptmp_004081"
     CDS             9160..9897
                     /gene="hisA"
                     /locus_tag="ID725_RS20405"
                     /old_locus_tag="pgaptmp_004081"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGV7852569.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            9879..10655
                     /gene="hisF"
                     /locus_tag="ID725_RS20410"
                     /old_locus_tag="pgaptmp_004082"
     CDS             9879..10655
                     /gene="hisF"
                     /locus_tag="ID725_RS20410"
                     /old_locus_tag="pgaptmp_004082"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGV7852570.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10649..11260
                     /gene="hisIE"
                     /locus_tag="ID725_RS20415"
                     /old_locus_tag="pgaptmp_004083"
     CDS             10649..11260
                     /gene="hisIE"
                     /locus_tag="ID725_RS20415"
                     /old_locus_tag="pgaptmp_004083"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGV7852571.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11357..12334)
                     /gene="wzzB"
                     /locus_tag="ID725_RS20420"
                     /old_locus_tag="pgaptmp_004084"
     CDS             complement(11357..12334)
                     /gene="wzzB"
                     /locus_tag="ID725_RS20420"
                     /old_locus_tag="pgaptmp_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGV7852572.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIESSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
BASE COUNT         3134 a         3261 c         3192 g         2839 t
ORIGIN      
        1 ataaattaat taaccagatg aatgttaatg gggaaattat tcatgactgg tggaatgcag
       61 accaataacc aaattctcta aattagaaca aatggttatt aatgaggatt tcgataaaaa
      121 agaataaaaa aaccggagcc aaatgttcat tcgactccgg tttattatta gaaatgatga
      181 ttatgctacg tcttcgtact gcggaaccgg gttacggaag ctcttggtga cgcaagccag
      241 gtaaatcaga ccgatagcag cccagatcag acccagaacc attgagcttt cttccaggtt
      301 aacccacagc gcaccaacgg tcagcgcacc acacatcggc aggaacagat actggaagtg
      361 atctttcagc gtcttattac gcttctcacg gatccagaac tgcgagatga ccgacaggtt
      421 aacgaaggtg aacgccacca acgcaccaaa gttgatcagc gccgtagcca ttaccaggtc
      481 gaagttgatt gccagcaggg caatcgcgcc aaccaggatg atgttcatcg ccggagtacg
      541 ccatttcggg tgtacataac cgaagaaact tttcgggaac acgccgtcac gacccattac
      601 gtacatcaga cgcgctacgc ctgcgtgcgc cgccataccg gacgccagta cggtaatggt
      661 ggagaagatc agcgcaccga cctggaacgc tttacccgca acgtacagca tgatttcagg
      721 ctgtgacgca tccggatctt taaagcgaga gatatccggg aagtacagct gcaggaagta
      781 agttgcaaag atgaagatca tgccgccgat cagcgcggtc aggaaaatcg cacgcgggat
      841 cacgcgctct gcatctttgg tttcttccga cagattgctg atgccgtcaa agccggtaaa
      901 ggagaagcac aggattgtcg ccccggtaat catcgggata acatgcgcat cgccagacca
      961 gaatggacga gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc cataaacaac
     1021 catgcccaga atcaccgcga tcagcactac ctgcaacacg acgattacag tgttgaagtt
     1081 cgctacggat ttcagactac gcaggttaaa ggcggtcata aaggccacca gcgccaccac
     1141 aaacatccac gatgggatgg aaggcaccag agcttcaaaa tagattttcg ccagcagaat
     1201 gttgatcatc ggcgcgaaca gatagtcgag cagagaagac caacccacca taaagccgac
     1261 agtcgggcta atggatttct gggcgtaagt atatgcagag ccagcagaag gatagcggcg
     1321 aaccagcttc ccgtagctca gagccgtaaa caggatcgca atcaatgcga acgcataggc
     1381 tgtcggcaca tgaccatccg tgaggcctga aacgatacca aaagtatcaa acagcgtcat
     1441 cggctgcata taggcaagac ccatcattac aaccggaact aacgtaagcg ttttacgcaa
     1501 ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg gtcattctcc tttacggtga
     1561 taaccgtcgc gtaagcaaaa aattgcccca tttttttgga ttcctcagcg acaacaactg
     1621 tcgattttta gtaaatatct atccggtacg aagcccggcc tcttggtatg aatgattggt
     1681 ttgaagcaaa aaataaccga cgctgatgaa acgtcggttt ttagtcattt tttgacagcg
     1741 gcgcattgtg cctaaatggg ggggaaatga caagagaatg agaggcttgt cataataatt
     1801 tttctttaaa tggctgattt tccgtcatca gattttgcat aaacaccggc gatcgcacta
     1861 tttgctaaaa tttcatcccg ccaccagccg ctggcaagtc ctgcggtctg ttcattccag
     1921 ccaatccaga cgggttcaaa gagagtttgg gcgaccactt tcttctcgat taacgcgcca
     1981 ctatcgagaa aacgttgcgc cagataacgg ggtaaatagc cgcagccgag gccgctaatt
     2041 tgcagctcca gcttggtttt aaaatcgaaa acggtaatgg cttcttgttc gtcgagaagt
     2101 tgtgaggccg tagaagcgcc tgcctgcgca gtgtccccca ccacaatcgc ccgatatcgt
     2161 ttaatgatac gacggtttaa tggttcttct tcctgggcta acggatgatg aggcgcgacc
     2221 gcaaagactt gttccagatc gcccaaccgc gagaaaccaa actcactgga agatggtggc
     2281 tcatgcatcg cgccaacaat aatatccgct cgcccctgag tcagggcatc ccatgaacca
     2341 ccgagtacgc cattgataaa cttcaggcgc gtcacgttat gatgttgata aaaagcttca
     2401 atgagtggcg caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac cagctcgttt
     2461 tcccagcctt catgcagttt gatcgcctgc ttttccagtt ctcgcacggt atgcagaact
     2521 tcccgcccct tctctaataa catttttccg gtacgggtga atttagcgcg gtgaccgcta
     2581 cgatcaagca attgaatatt aaggtcgctt tccagcttgt gaacggtgta actcagagcg
     2641 gacggcgtct tgtagagttt agccgacgcc gccgcaaaac tgccttcttt ttctaaggca
     2701 tcgaggatca tcaacacatc cagcagtggt ttcatacacg cccccttgca gtgcatgcgg
     2761 cgtcctgctg gcgtgatcgt ttactccagc ggcattacca gcggatcggg atactggtat
     2821 tcaaatccca gttcattaca aatccgactg ccatcaataa tcttaccttt gccgctgtcc
     2881 agactatttc tgaattgcgg aggttccagc cccagtaaac gggccatctg cggatagaaa
     2941 acattacgcg cagggtgagc tggcgcacat atattataga tgtgtccgcc tttaggtgcc
     3001 tgtaacaaca gagtgatagc gccaataaca tcttctaaat ggactaaatt aacaccatgt
     3061 tcaccatcag gcgcggtttt tccggcaaag aagcgtccgg gatgacgccc cggtccgacc
     3121 aggcccgcaa gacgcagaat atcgaccgaa gtaccgggta aattgtgcag ccagtcttcg
     3181 agttcttcta atactcgtcc actgttggtc accggattac gcggggtggt ttctttcacc
     3241 gtgccttgcg cgtcgccata gacagacgtt gaactggtaa aaataattcg gggaatacga
     3301 tgggccagcg cgctatccac taactcttgt accgcttgta aatagaactc atcgccgggg
     3361 ccgctacgac gggccggaag cgtaatgacc agcgcatcgg catccatcag ggcatccaga
     3421 tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca gatagctatc aatgccactc
     3481 attcgggccg cttcgacacc atcttgtgtg gttttactcc cggtgacttg ccagcctcgc
     3541 gctgaaagtg acatcgccag cggcatgcct aaccacccta acccgacaat tgcgaccttt
     3601 ttcatccgtt atctcctgat acaccttaga tctataaggc tacgctagcg tatcaaaact
     3661 gacaattcat tctatgaatg aaatcgaaac tgttagcaaa tataccattg cctgatgcgc
     3721 tccgcttatt agtcctacaa aacatcaata tattgaattt aggcaacatt tgtagaccag
     3781 ataaggcatt cacgccgcat ccggcaggaa caaagcccac tttagttaca gaacacaatg
     3841 ttcttgaggg ttttaatgac atattttcac gattactttt ttaacctagt cactcttagc
     3901 cccctccttt ccccgctcat tcattaaaca aatccatcgc cataaaatat ataaaaaagc
     3961 ccttgctttc taacgtgaaa gtggtttagg ttaaaagaca tcagttgaat aaacattcac
     4021 agagactttt atgacacgcg ttcaatttaa acagcaccat catcaccatc atcctgacta
     4081 gtctttcagg cgatgtgtgc tggaagacat tcagatcttc cagtggtgca tgaacgcatg
     4141 agaaagcccc cggaagatca ccttccgggg gcttttttat tgcgcggttg ataacggttc
     4201 agacaggttt aaagaggaat aagaaaatga cagacaacac tcgtttacgc atagctatgc
     4261 agaaatccgg ccgtttaagt gatgactctc gcgaattgct ggcgcgctgt ggcattaaaa
     4321 tcaatcttca cacccagcgc ctgatcgcga tggcagaaaa catgccgatt gatattctgc
     4381 gcgtgcgtga cgacgacatt cccggtctgg taatggacgg cgtggtagat cttgggatta
     4441 tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg cgcccagggt gaagatccac
     4501 gctactttac cctgcgtcgt ctggatttcg gcggctgccg tctttcgctg gcaacgccgg
     4561 ttgatgatgc ctgggacggc ccgctctcct taaacggtaa acgtatcgcc acctcttatc
     4621 ctcacctgct caagcgttat ctcgaccaga aaggcatctc ttttaaatcc tgcttactga
     4681 acggttctgt tgaagtcgcc ccgcgtgccg gactggcgga tgcgatttgc gatctggttt
     4741 ccaccggtgc cacgctggaa gcaaacggcc tgcgcgaagt cgaagttatc tatcgctcga
     4801 aagcctgcct gatacaacgc gatggcgaaa tggaagaatc caaacagcaa ctgatcgaca
     4861 agctgctgac ccgtattcag ggtgtgatcc aggcgcgcga atcaaaatac atcatgatgc
     4921 acgcaccgac ccaacgtctg gatgaagtca tcgccctgct gccaggtgcc gaacgcccaa
     4981 ccattctgcc gctggcgggt gatcaacagc gcgtagcgat gcacatggtc agtagcgaaa
     5041 ccctgttctg ggaaacgatg gaaaaactga aagcgctggg tgccagttca atcctggtcc
     5101 tgccgattga gaagatgatg gagtgatcgc catgagcttt aacacaatca ttgactggaa
     5161 tagctgtact gcaaagcaac aacgccagct gttaatgcgc ccggcgatct ccgcttctga
     5221 aagcattacc cgcactgtta acgatattct cgatagcgtg aaagcacgcg gtgatgacgc
     5281 cctgcgggaa tatagcgcga agtttgataa aaccacggtt accgcactga aggtgtctgc
     5341 tgaggaaatt gccgccgcca gcgaacgcct gagcgacgag ctaaaacagg cgatggcggt
     5401 ggcagtaaag aatattgaaa ccttccacac tgcgcaaaaa ctgccgccgg tagatgtaga
     5461 aacgcagcca ggcgtgcgtt gccagcaagt cacgcgcccg gtagcttcag ttgggttgta
     5521 tattcctggc ggctccgccc cgctcttctc aacggtatta atgctggcaa ctccggcgcg
     5581 tattgcgggc tgtaaaaaag tggtgttgtg ctcaccgccg ccgattgccg atgagatcct
     5641 ttatgcggcg cagctgtgcg gtgtgcagga cgtgtttaac gtcggcggcg cacaggccat
     5701 tgccgcgctg gcgtttggta cggaatctgt gccgaaagtg gacaaaatct tcgggccggg
     5761 taacgccttt gtcaccgagg caaaacgtca ggtgagccag cgtctggacg gtgcggcgat
     5821 cgatatgccc gcaggcccgt cggaagtgtt ggtcattgcc gacagcggcg caacgccgga
     5881 tttcgtggct tctgatttgc tttctcaggc tgaacacggc ccggactcac aggtgatttt
     5941 actgacgcct gacgctgata tggcgcatca agttgccgaa gccgtcgaac gccagttagc
     6001 agaactgccg cgtgccgaaa ccgcccgcca ggcactgaac gccagccgcc tgatcgtgac
     6061 taaagattta gcgcagtgcg tggagatctc caaccagtac ggcccggagc acctgatcat
     6121 tcagacccgc aacgcccgcg aactggtcga tggcatcacc agcgccggtt cggtatttct
     6181 tggtgactgg tcaccggaat cggcaggtga ttacgcctcc ggaaccaacc atgttctacc
     6241 gacttacggc tacaccgcca cctgttccag cctcggactg gcggatttcc agaagcggat
     6301 gaccgtgcag gaactgtcga aagtaggttt ctccgcgctg gcttcgacca ttgaaacact
     6361 ggccgccgcc gagcgcctga ccgcccacaa aaatgccgtt actttgcgtg ttaacgccct
     6421 taaggagcaa gcatgagcac cgtgactatt accgatttag cgcgtgaaaa cgtccgcaac
     6481 ctgacgccgt atcagtcggc gcgtcgtctg ggcggtaatg gcgacgtctg gctgaacgcc
     6541 aacgaatatc ccacagccgt ggagtttcag cttactcagc aaacgctcaa ccgctacccg
     6601 gaatgccagc cgaaagcggt gattgaaaat tacgcgcaat atgcaggcgt aaaaccggaa
     6661 caggtgctgg tcagccgtgg cgcggacgaa ggtattgaac tactgattcg cgctttttgc
     6721 gaaccgggta aagacgccat cctctactgc ccgccaacgt acggcatgta cagcgtcagc
     6781 gctgaaacca ttggcgtcga gtgccgcaca gtgccgacgc tggaaaactg gcaactggac
     6841 ttacagggca tttccgacaa gctggacggc gtaaaagtgg tctatgtttg cagccccaac
     6901 aacccaaccg ggcaactgat caatccgcag gattttcgca ccctgctgga gttaacgcgc
     6961 ggtaaggcga ttgtggttgc cgatgaagcc tatatcgagt tttgcccgca ggcatcgctg
     7021 gctggctggc tggcggaata tccgcacctg gctattttgc gcacactgtc gaaagctttt
     7081 gctctggcgg gccttcgttg cggatttacg ctggcaaacg aagaagtcat caacctgctg
     7141 atgaaagtga ttgcccccta cccgctctcg acgccggttg ccgacattgc ggcccaggcg
     7201 ttaagcccgc agggaattat cgccatgcgc gaacgggtgg cgcaaattat tgcagaacgc
     7261 gaatacctga ttgccgcact gaaagagatc ccctgcgtgg agcaggtttt tgactctgaa
     7321 accaactaca ttctggcgcg ctttaaagcc tccagtgcag tgtttaaatc tttgtgggat
     7381 cagggcatta tcttacgtga tcagaataaa caaccctctt taagcggctg cctgcgaatt
     7441 accgtcggaa cccgtgaaga aagccagcgc gtcattgacg ccttacgtgc ggagcaagtt
     7501 tgatgagtca gaagtatctt tttatcgatc gcgatggaac cctgattagc gaaccgccga
     7561 gtgattttca ggtggatcgt tttgacaaac tcgcctttga accgggcgtg atcccgcaac
     7621 tgctgaagct gcaaaaagcg ggctacaaac tggtgatgat cactaatcag gatggtctgg
     7681 gaacacaaag tttcccgcag gcggatttcg atggcccgca caacctgatg atgcagatat
     7741 tcacctcgca aggcgtacag tttgatgaag tgctgatttg tccgcacctg cccgccgatg
     7801 agtgcgactg ccgtaaaccg aaagtaaaac tggtggagcg ttatctcgct gagcaagcga
     7861 tggatcgcgc caacagttat gtgattggcg atcgcgcgac cgatattcaa ctcgctgaaa
     7921 acatgggcat taatggttta cgctacgacc gcgaaaccct gaactggcca atgattggcg
     7981 agcaactcac cagacgtgac cgttacgctc acgtagtgcg taataccaaa gagacgcaga
     8041 ttgacgttca ggtgtggctg gatcgtgaag gtggcagcaa gattaatacc ggcgttggct
     8101 tctttgatca catgctggat cagatcgcca cccacggcgg tttccgtatg gaaatcaacg
     8161 tcaaaggcga cctctatatc gacgatcacc acaccgtcga agataccggc ctggcgctgg
     8221 gcgaagcgtt aaaaattgcc ctcggcgata aacgcggtat ttgccgcttt ggttttgtgc
     8281 tgccgatgga cgaatgcctt gcccgctgcg cgctggatat ctctggtcgc ccgcacctgg
     8341 aatataaagc cgagtttacc taccaacgcg tgggcgatct cagcaccgag atgatcgagc
     8401 acttcttccg ttcgctctct tacaccatgg gcgtgacgct ccacctgaaa accaaaggta
     8461 aaaacgatca tcaccgtgta gagagcctgt tcaaagcctt tggtcggacc ctgcgccagg
     8521 ccatccgcgt ggaaggcgat accctgccct cgtcgaaagg agtgctgtaa tgaacgtggt
     8581 gatccttgat actggctgcg ccaacctgaa ctcggtgaag tctgccattg cgcgtcacgg
     8641 ttatgaaccc aaagtcagcc gtgacccgga cgtggtgttg ctggccgata aactattttt
     8701 acccggcgtt ggcactgcac aagcagcgat ggatcaggtg cgtgagcgcg agctgtttga
     8761 tctcatcaaa gcctgtaccc aaccggtgct gggcatctgc ttagggatgc aactgctggg
     8821 gcggcgcagt gaagagagca acggcgtcga tttgctgggc atcatcgacg aagacgtgcc
     8881 gaaaatgacc gactttggtc tgccgctgcc gcatatgggc tggaatcgcg tttacccgca
     8941 ggcaggcaac cgcctgtttc aggggattga agacggcgcg tacttttact ttgttcacag
     9001 ctacgcaatg ccagtcaatc cgtggaccat cgcccagtgt aattacggcg aaccattcac
     9061 cgcggcggta caaaaagata acttctacgg cgtgcagttc catccggagc gttctggtgc
     9121 cgctggcgct aagttgctga aaaacttcct ggagatgtga tgattattcc ggcattagat
     9181 ttaatcgacg gcactgtggt gcgtctccat cagggcgatt acggcaaaca acgcgattac
     9241 ggtaacgacc cgctgccgcg cttacaggat tacgccgcac aaggtgccga agtgctgcat
     9301 ttggtggatc tgaccggggc aaaagatccg gcaaaacgtc aaatcccgct gattaaaacc
     9361 ctggtcgctg gcgttaacgt tccggtgcag gttggcggcg gcgtgcgtag cgaagaagac
     9421 gtggcggcgt tactggaagc tggggttgcg cgcgtggtgg ttggctccac tgcggtgaaa
     9481 tcaccagaaa tggtgaaagg ctggtttgaa cgtttcggtg ccgatgcctt agtgctggcg
     9541 ctggatgtcc gtattgacga gcaaggcaac aagcaggtgg cggtcagcgg ctggcaagag
     9601 aattcgggcg tgtcactgga acaactggtg gaaacctatc tgcccgtcgg cctgaaacat
     9661 gtgctgtgta ccgatatctc gcgcgacggc acgctggcag gctctaacgt ctctttatat
     9721 gaagaagtgt gcgccagata tccgcaggtg gcatttcagt catccggcgg cattggcgac
     9781 attaacgatg ttgctgccct gcgtggcact ggcgtgcgcg gcgtaatagt tggtcgtgca
     9841 ttactggaag gtaaattcac cgtgaaggag gccatctcat gctggcaaaa cgcataattc
     9901 catgtctcga cgttcgtgat ggtcaggtgg taaaaggcgt acagtttcgc aaccatgaaa
     9961 tcattggcga tatcgtgccg ctggcaaaac gctacgccga agaaggtgca gacgaactgg
    10021 tgttctacga tatcaccgct tccagcgatg gccgtgtggt agataaaagt tgggtatctc
    10081 gcgtggcgga agtgatcgac attccgttct gcgtggcggg tgggattaag tctctggaag
    10141 atgccgcgaa aattctttcc tttggcgcgg ataaaatttc catcaactcc cctgcgctgg
    10201 cggacccgac gttaattact cgcctggcgg atcgatttgg cgtgcagtgt attgtggtgg
    10261 gtattgatac ctggtacgac gctgaaaccg gtaaatatca tgtgaatcaa tataccggcg
    10321 atgaaagccg cacccgcgtc actcagtggg aaacgctcga ctgggtacag gaagtgcaaa
    10381 aacgcggtgc cggagaaatc gtcctcaaca tgatgaatca ggacggcgtg cgtaacggtt
    10441 acgacctcga acaactgaaa aaagtgcgtg aagtttgcca cgtcccgctg attgcttccg
    10501 gtggcgcggg caccatggaa cacttcctcg aagccttccg cgatgccgac gttgacggcg
    10561 cgctggcagc ttccgtattc cacaaacaaa taatcaatat tggtgaatta aaagcgtacc
    10621 tggcaacaca gggcgtggag atcaggatat gttaacagaa caacaacgtc gcgaactgga
    10681 ctgggaaaaa accgacggac tgatgccggt gattgtgcaa cacgcggtat ccggcgaagt
    10741 gttaatgctg ggctatatga acccggaagc cttagacaaa accatcgaaa gcggcaaagt
    10801 caccttcttc tcgcgcacta aacagcgact gtggaccaaa ggcgaaacgt cgggcaattt
    10861 cctcaacgta gtaaatattg cgccggactg cgacaacgac acgctactgg tactggcgaa
    10921 tcctatcgga ccaacctgcc acaaaggcac cagcagctgc ttcggcgaca ccgctcacca
    10981 gtggctgttc ctgtatcaac tggaacaatt gctcgccgaa cgcaaatctg ccgacccgga
    11041 aacctcctac accgccaaac tgtatgccag cggcaccaaa cgcattgcgc agaaagtggg
    11101 cgaagaaggc gtggaaaccg cactggccgc gactgtacat gaccgctttg agctgaccaa
    11161 cgaggcgtct gacttgatgt atcacctgct ggtattgttg caggatcagg atctggattt
    11221 aacaacggtg attgagaacc tgcgtaaacg tcatcagtga gttgcggggt aagcggatgc
    11281 gatattgttg tcgcatccgg caaaaaaaac gggcaaggtg tcaccaccct gccctttttc
    11341 tttaaaaccg aaaagattac ttcgcgttgt aattacgcag agcgttacgc cccagcacaa
    11401 tccccgcgcc aaccatgccg cccagcagca ccgccagaat caaggtgatt gcctttttcg
    11461 gactatcgcg acgaataggt aacgtcggtt tcattacata gcggtaagca tgaatatcaa
    11521 gatcatcaac ctttaagttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
    11581 gtgagaacac caacggacgg gtcgcctcat gcttaatcat cgactccagc gcttcgctcc
    11641 ccaaaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgcca
    11701 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agctctttct
    11761 gctcctgcgc aactacttcc tgcgttctta aagagtcctg caagtttttc cgtcccagag
    11821 caatgttgtc tttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
    11881 tgtattgggc caacttcatt tgtgctccct ctgcagtttg cccaacataa gaaacagtca
    11941 atggtaattg ctggttctta acagaagatt cgatggtaag tttttctggt tcttcctgat
    12001 tatccagcgt ttctgctaat gctgagaagg cagaactgaa gcgaccaatc aacgtctcct
    12061 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
    12121 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
    12181 ctactgccaa ataaccaata gccagagcaa tagccacaat gacggaaatg atgattgtca
    12241 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
    12301 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
    12361 aagtccgcat agtttagcga taatccctgt gaatgctata ggataagtga taaaaataat
    12421 gaatta
//
LOCUS       DABSYT020000119        12340 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.119, whole genome shotgun sequence.
ACCESSION   DABSYT020000119 DABSYT020000000
VERSION     DABSYT020000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.119"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..1093
                     /locus_tag="ID725_RS20425"
                     /old_locus_tag="pgaptmp_004085"
     CDS             <1..1093
                     /locus_tag="ID725_RS20425"
                     /old_locus_tag="pgaptmp_004085"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGV7852573.1"
                     /translation="ENCQTGVAYPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLC
                     DQLEQQSLTSLDAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVDAL
                     KQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKP
                     FELPEGWEWCLFEDIIDIQSGITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIE
                     IPIEEKEKYQVVKGDLLITEGGDWDTVGRTTVWCHDWYIANQNHVFKGRNIGQDVDPY
                     WLETYMNSPFSRQYFANASKQTTNLASINKTQLRGCPVAIPPSSEAKKIMSKLHIFYK
                     LCEELKNHIQSAQQTQLHLADALTDAAVN"
     gene            1314..1655
                     /gene="symE"
                     /locus_tag="ID725_RS20430"
                     /old_locus_tag="pgaptmp_004086"
     CDS             1314..1655
                     /gene="symE"
                     /locus_tag="ID725_RS20430"
                     /old_locus_tag="pgaptmp_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HGV7852574.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(1702..2865)
                     /locus_tag="ID725_RS20435"
                     /old_locus_tag="pgaptmp_004087"
     CDS             complement(1702..2865)
                     /locus_tag="ID725_RS20435"
                     /old_locus_tag="pgaptmp_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HGV7852575.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRTVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(2913..3794)
                     /locus_tag="ID725_RS20440"
                     /old_locus_tag="pgaptmp_004088"
     CDS             complement(2913..3794)
                     /locus_tag="ID725_RS20440"
                     /old_locus_tag="pgaptmp_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HGV7852576.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            complement(3891..4055)
                     /locus_tag="ID725_RS20445"
                     /old_locus_tag="pgaptmp_004089"
     CDS             complement(3891..4055)
                     /locus_tag="ID725_RS20445"
                     /old_locus_tag="pgaptmp_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGV7852577.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            4232..5644
                     /locus_tag="ID725_RS20450"
                     /old_locus_tag="pgaptmp_004090"
     CDS             4232..5644
                     /locus_tag="ID725_RS20450"
                     /old_locus_tag="pgaptmp_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGV7852578.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(5887..6844)
                     /locus_tag="ID725_RS20455"
                     /old_locus_tag="pgaptmp_004091"
                     /pseudo
     CDS             complement(5887..6844)
                     /locus_tag="ID725_RS20455"
                     /old_locus_tag="pgaptmp_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            7311..8543
                     /gene="mdtM"
                     /locus_tag="ID725_RS20460"
                     /old_locus_tag="pgaptmp_004092"
     CDS             7311..8543
                     /gene="mdtM"
                     /locus_tag="ID725_RS20460"
                     /old_locus_tag="pgaptmp_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGV7852579.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            8584..9855
                     /locus_tag="ID725_RS20465"
                     /old_locus_tag="pgaptmp_004093"
     CDS             8584..9855
                     /locus_tag="ID725_RS20465"
                     /old_locus_tag="pgaptmp_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGV7852580.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            9971..11122
                     /locus_tag="ID725_RS20470"
                     /old_locus_tag="pgaptmp_004094"
     CDS             9971..11122
                     /locus_tag="ID725_RS20470"
                     /old_locus_tag="pgaptmp_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGV7852581.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVVETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            11132..11899
                     /gene="yjiL"
                     /locus_tag="ID725_RS20475"
                     /old_locus_tag="pgaptmp_004095"
     CDS             11132..11899
                     /gene="yjiL"
                     /locus_tag="ID725_RS20475"
                     /old_locus_tag="pgaptmp_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGV7852582.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            11896..12162
                     /locus_tag="ID725_RS20480"
                     /old_locus_tag="pgaptmp_004096"
     CDS             11896..12162
                     /locus_tag="ID725_RS20480"
                     /old_locus_tag="pgaptmp_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGV7852583.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
BASE COUNT         3052 a         3067 c         3014 g         3207 t
ORIGIN      
        1 tgaaaattgt cagacagggg ttgcctatcc agcaatcaat gacaaacaat ttttttctgg
       61 aataactcca gttcctccat ccttggaaca agttcgcatc gcaaacaaaa tcaaagaatt
      121 aatgtccctc tgcgaccaac tggaacagca atccctgacc agtctggacg cacatcaaca
      181 actggttgaa accctgttgg gaacgcttac agacagccaa accgccgagg aactggctga
      241 aaactgggcg cgtattagcg agtatttcga cactctattt accaccgaag ccagcgtgga
      301 tgcgttaaaa cagaccattc tgcaactggc cgtaatgggc aaacttgtgc cgcaggatcc
      361 gaatgatgaa ccagcctctg aactgctcaa acgaattgcg caggaaaaag ctcaactggt
      421 gaaagaagga aaaataaaaa aacaaaaacc gttgccgcca attagcgatg aggaaaaacc
      481 gtttgaactt ccggaagggt gggagtggtg tttatttgaa gatattattg atattcaaag
      541 tggtatcact aaaggaagaa atttatcaaa tagaactttg gtaaaagttc cttatttacg
      601 tgttgcaaac gtccaacgcg gatatcttga tcttacggaa attaaacaga ttgaaatccc
      661 tattgaggaa aaagaaaaat atcaagtagt caagggagat ttattgataa cagaaggcgg
      721 cgactgggat acagtcggga gaactacagt atggtgtcat gactggtata tagcaaatca
      781 aaaccatgta ttcaaaggac gaaatatagg gcaagatgtt gatccatatt ggttagaaac
      841 atatatgaat agcccattct caagacaata ttttgctaac gcaagtaagc aaaccactaa
      901 tttagcttct attaataaaa cccagctcag aggttgtcct gttgctattc ctcctagctc
      961 agaagcaaaa aaaataatga gtaaactaca tattttttat aaactatgtg aagaattaaa
     1021 gaatcatatc caatccgccc agcaaaccca actacacctt gcagatgcac tcactgacgc
     1081 ggcggtaaac taatttacct gccctcgaaa aaccaataaa gcggctaaat atccgcttta
     1141 ttcacatcac caaaatttat atttcacgct aattttcatc ttattttcat taatttttag
     1201 ctggactttc cccatattta ctgatgatat atccaggtat ttagcgcggt gcggatgtgc
     1261 gccaacacac ccgcaccgct aatcacaatc actatttata gggaatagca gttatgactg
     1321 atacgcattc tattgcacaa ccgttcgaag cagaagtctc cccggcaaat aaccgtcaat
     1381 taaccgtgag ttatgcgagt cgttacccgg attacagccg tattcccgcc atcaccctga
     1441 aaggtcagtg gctggaagcc gccggttttg ccaccggcac ggtggtagat gtcaaagtga
     1501 tggaaggctg tattgtcctc accgcccaac cacccgccgc cgccgagagc gaactgatgc
     1561 agtcgctgcg ccaggtgtgc aagctgtcgg cgcgtaaaca aaggcaggtg caggagttta
     1621 ttggggtgat tgcgggtaaa cagaaagttg cctgatacat tgacccggta gcgccaggac
     1681 taccgggcat ttttcgctgc gttataccaa cgcgctctcc atctgttttt caaatgcctg
     1741 acgaattaac cccataatgt aaggaagttg tgcgaggtca ctgaaaccaa tttccacatc
     1801 accattgccc catcgcccaa catttgtcac atctttggca atacctttcg gatcgaccag
     1861 ttcatgaaac tgcatattaa gcgacaatcg cagtcggctt ttttgcggca caacatcaac
     1921 aaaattcgtt tccgctttaa acgcaatata gagcttcaat acttcctgcg tgatccctgc
     1981 gtccaggcgc ataatttcat cgcgaagatg atcgaataac acccggctat gcgaaccatc
     2041 agcaagaaaa ggataatcac tcaggctgta actggttttg ttctcaggct gtccccgata
     2101 aacagctaaa acctcttcag gaagagaagg cgcttgccag acctgaaccg caagctccgc
     2161 cagacgatcg gctcgggcgt gaatggcagc ttcatcccat ttttcgcact gtccaagacc
     2221 gatattcaaa tagagcgggc tatgcttaaa gccgtcttca atatcgcgtt tttctgcgaa
     2281 aggtctgtca ctgtaacggg agttatagcg cgttaaagtg agattcccca acgtatgcaa
     2341 cagttcttta tgaatacgct gccagtcact tcccagctct tcgcgccatt tagccgacaa
     2401 attttcgttc tgcggcataa tatgttcaat cgtaaactct tccacgcgct ctctgcgttt
     2461 atcgttttcc agtcgtcgta accagtagct acgactacgg aagttataga gatcgcgaac
     2521 ttttaattcc cgtttaaatt catcatcgtt ggggaaacga cgatatgaag gtagattcaa
     2581 aaaatgtacc tgaatacttt ccagatattt ttctttatta atgaccttat aaaaagtagc
     2641 aaacgtcttg ttaagagaat ttgtcggaat cgcacatact gtacggcgga aaacataaga
     2701 ttcaattaaa cgaattatgc tcaggaaatc ttcgtgagac aaatcgtcat ttttatagtc
     2761 atgataaagc gccagtaaga aaggatacgc cacatcaacc tttaactcgc gcaaatcctg
     2821 aaaagccgta gcaagcgatt tgtcactttc ttttcccaat gccattgcac agtaatactc
     2881 cgcgtagata tgaatatctt caaccagccg atctacgcct ttttcagcaa cactctggct
     2941 gcgggcatgg agtttaaatg cctcatagac ttcatctgtc cgagggatct ccccgttttt
     3001 accgtcagat aatgacgcat aaaactgtca aaatattcgc tgtaaccttg ctgaccacaa
     3061 gcgacttcca ttggacgcca gtgatcttca tacaaccggg tttgatgctc tggttcgagg
     3121 cccatcagaa taaaattgcg caccagatcg gcctgactta acgccttacc ggtggagttc
     3181 atactttcaa aaatcagttg cggattatcc tgaccacgat taagcgccac atcgacaatt
     3241 aataactttg ctaacccacg acataagggg atcaagtcat caccgagttt ggcaatttgt
     3301 tcatcaaaga aagtaaagtt ttccattatt cgatgggaat agttttctgg cattggtctt
     3361 tgttttatta acgccagtaa actgtcttta tcagtctcgg tcagcagtaa tttaaaacct
     3421 ttctcgccgg actcataagg attgagcaaa taataattac gcaatttcat tgccgaaaag
     3481 ccatcaaaaa cttcgtcttc gccaagatgg cgcgataaag cctcaatcag caacattgcg
     3541 gtcgtcagcc gctgctgacc atcaatgacc agcaacggag aaataccaga aacctgatac
     3601 aatccctgct ctatataaac aaccgaaccg ataaaatgcg ctgatatatc gtcacgcttt
     3661 cctgcacgaa tgatgtcgtc ccaaagttgc cgacattgtt gttctgtcca tgaataagtc
     3721 cgttgataaa tgggaataac aaactgctgc gaacgtttta aaaaatcgag taggcgagct
     3781 tctgtagctt tcattctgac gtctttccgt gaaacgatga tttcgcaata ataaccactt
     3841 ccttatggag gttatagaga aatcctgttt atccgcgacg aacatacccc tcactccaca
     3901 tcctccctgc gtaacccaat atccctcaac cgttcatcac tcatctgctg taacacccgt
     3961 cgggcctgca tttgccgccg ccaacgcctc actgcctgcc agagttgcac aagtctgata
     4021 aacggcgttt tggctttgtt ttcgtgaaat tccatctgct gcctcctcat atttcagagg
     4081 cttcatcatc cgtcttctgg cagttcacaa tacagatgca aatattagtt ttgtttaaca
     4141 tacagacagg ttaaaacagc atctgcatcc ggttttttag cctcatctgt accggttttt
     4201 ctttctgtat ggtccaaata aggaatacag catgacgcgt tatcaacatc tggcgactct
     4261 acttgccgag cggattgagc aagggctgta tcgtcacggg gagaaattgc cgtcggtgcg
     4321 cagcttaagt caggagcacg gcgtcagcat cagcaccgtg cagcaggcgt accagacgct
     4381 ggagacgatg aagctcatca ctccgcagcc gcgttcgggt tattttgtcg cacaacgtaa
     4441 agcccagccg ccagtgccgc cgatgacgcg tccggtgcag cgcccagtgg aaattaccca
     4501 gtgggatctg gtgctggata tgctggtggc gcatagcgac agttccattg ttccgttaag
     4561 caaaagcacg ccggatgtcg aaacgcccag cctgaaaccg ctgtggcggg agctaagccg
     4621 ggtggtgcag cataatctgc aaaccgttct cggttatgac ttgctagccg gtcagcgggt
     4681 attgcgcgag caggttgccc gcctgatgct cgacagcggc tcggtggtca ctgccgatga
     4741 catcatcatc accagcggct gccataactc gatgtcgcta gcgttaatgg cggtgtgtaa
     4801 accgggcgat attgtcgcgg tcgaatcccc ctgttattac ggttcaatgc agatgctgcg
     4861 cggcatgggc gtgaaagtga ttgaaatccc aaccgatcca gaaacaggca tcagcgttga
     4921 agcgctggaa ctggcgctgg aacagtggcc gattaaaggc atcattctgg tgccaaactg
     4981 taataatccg ctgggattta ttatgccgga cgcgcgcaaa cgggccgttc tctctctcgc
     5041 tcagcgtcat gatattgtga tttttgaaga tgatgtctac ggcgaactgg caacggagta
     5101 tccgcgcccg cggaccattc attcctggga tatcgacggg cgagtgctgt tgtgcagctc
     5161 gttcagtaaa agtattgctc caggcctgcg cgtgggttgg gtcgcaccgg ggcgttatca
     5221 cgataaactg atgcatatga aatacgccat cagcagcttt aatgtgccgt ccacgcaaat
     5281 ggcggcggca acgtttgtgc tggaaggtca ctatcatcgc catatccggc ggatgcggca
     5341 gatctatcag cgcaatttgg cgctttatac ctgctggata cgggaatatt ttccctgcga
     5401 aatctgtatt acgcgcccga aaggcggatt tttactgtgg atagaattgc ctgaacaggt
     5461 cgatatggtc tgcgtcacgc ggcagctgtg ccgcatgaaa atccaggtgg cggcaggctc
     5521 gattttctca gcttccggca aataccgtaa ctgcctgcgc atcaactgtg ctttgccact
     5581 cagcgaaact tatcgcgaag cgctgaagca aattggcgag gccgtgtatc gggcaatgga
     5641 ataaattaga attagtggcg aagcacctga tttattctca cgaatcgcct gtgactcgtt
     5701 ttatctttgc aggatcatag ccacgttccg gcatggctta ggtaaagtgc gctttgttca
     5761 tgccggatgc catgtgaacg ccttaccagg tctacaaaat ctttaaagtt caatatattg
     5821 aatgggcttt gtagacctga taagcgtagc acatcaggct gctttgcgtt tgtcatccgt
     5881 ctctggttag ctcttgagcc atgaatagcg ctcacgctca ggcaagggct gccttaatgc
     5941 ctgcatttgc tctgccgaaa gtccggtaat cttctgtatc cattcaggat ctgccccatt
     6001 ctgcaacaac aatcgaagaa tgcgctgttc cccttcttcc cttcccaaca gccatccatc
     6061 attatgaatt cgctctgcaa tcgtcattat gaattcgctc tgcaatcgtc attattcgct
     6121 ctctgtgttg aggcattcga ctggttagtt cactgataaa ctcattaaaa cgctcttcat
     6181 cgccagtcag taaaatgtaa tttaacagcg cagttatctg gctgtcatta gcacactctg
     6241 taaccagtaa tactaccagt tgatcgataa gccccatcag atcgcgctgg cgaatatgct
     6301 tttggatcaa ctccaacagg gcgactctgc ggtgctgcac aatctcgtcg tccggcacga
     6361 cagtgatatc caccagcggg aacgcggcgt tataaagctt ccgtgcagta gtcgggtcgg
     6421 caaattcgtc caaccagcac aaagaccagg gataaggact acggctaccg tgataaaaca
     6481 gcatcgggat gaccagcggt agtggccggc gtttatcatg ctctatatgg cgctgcatca
     6541 ctgccatgga atagcgcatc aggcgaaagg ccatatgaat atcctcacgg ctctgatgtt
     6601 caatcaccac atagatatag ccatcgcctt cccgggtctt taccgaccac agaatatcgg
     6661 agtgtagcgc ccgcaatttt tcatcgacaa agctggcgga ttccagtttt aagctgtcga
     6721 gatcgcacag ttcacgtaaa tctttgggta agtggatctc cataaaatcc cgcgcggtgt
     6781 cagggtgcat gagaaaagat ttaaataatg cgtcatgcgg cgtgctggtc gtgaagtttg
     6841 tcatggcgat ccgtcacctg ttgataagat ggcccgactt tagcgactaa caaaaccatt
     6901 ctcacccggc aatttctcgt ttcccgaggc gctttccagg ctatttttgt tcgtctgcaa
     6961 acgatacatt tttccggaaa ctgactcgcg ttttacatgt cctgtacccg gcagatattc
     7021 ccccttccac cgcgctcttt tgattaatcg caaattttaa ttacacttaa ggtgtatatt
     7081 ttctatgcaa cccatcaatt caagaggtgt aatgtgctga tgactatttg taatcgttat
     7141 acattctgac ccgaagtcag aaggtatttc tctgtctgtg tgttcacagg cagtgtggtt
     7201 gattacatga aattcagtac atttgtcctt ctcgcctacc tttcgtcatt accgacggta
     7261 ttgaatttcg ttttccccgt tggggttctc cggacaagga gttgtttgtt atgccacgtt
     7321 tttttgcccg ccatgccgcc acgctgtttt tcccgatggc gttgattttg tatgactttg
     7381 ccgcctatct ttcgacggac ctgatccagc ctgggatcat taatgtggtc cgtgatttta
     7441 atgccgatgt cagtctggct ccggctgccg tcagtctcta tctcgctgga ggcatggcgt
     7501 tgcagtggct gctggggccg ctttccgaca ggattggccg caagccagtg ctgattaccg
     7561 gggcgctaat atttaccctt gcctgcgctg cgacaatgtt cacaacgtct atgacacagt
     7621 ttcttatcgc gcgtgcaatt cagggcacca gtatctgttt tattgccacc gttggttatg
     7681 tcacggtgca ggaggcgttc ggccagacaa aagggatcaa gttgatggcg attatcacct
     7741 ccatcgtact gattgcgccg attatcggcc cgctttccgg cgcagctctg atgcactttg
     7801 tgcactggaa agtccttttt gccatcattg cggttatggg ttttatctca tttgttggct
     7861 tactgctggc gatgccagag acggtgaagc gcggcgcggt tccatttagc gccaaaagcg
     7921 tcttgcgcga ttttcgtaat gtcttttgca atcggctgtt cctctttggc gcagcaacca
     7981 tctctttaag ctatatcccc atgatgagtt gggtggctgt ctcgccggtg atcctgattg
     8041 acgcaggcgg cttaacgacc tcgcagttcg cctggacaca ggttccggtg ttcggcgcgg
     8101 tgattgtcgc gaatgccatc gtggcgcgtt tcgttaaaga tccgaccgaa ccgcgcttta
     8161 tctggcgtgc cgtacccatt caactggtcg gcctcgcgct gttgattatc ggcaatctgc
     8221 tgtcaccgca cgtctggctg tggtcggtgc tgggcaccag tctgtatgct ttcgggattg
     8281 gtttgatctt cccgacctta ttccgcttta cgctgttttc caataactta ccgaaaggga
     8341 ccgtctccgc atcgctgaat atggtgatcc tgatggtgat gtctgtctcg gtcgaaattg
     8401 gccgctggct gtggtttaac ggcggtcgct tgccgtttca tctgttagcc gttgtggcgg
     8461 gcgttatcgt cgttttcacc ctggccggat tgcttaatcg cgtgcgccaa catcaggctg
     8521 ccgagctggc agaggagcag tgatttttgc gcgatcctgc cgtcaggctc tattcttaac
     8581 gttatgaata aactcattga actcagacgc gccaaaatgt tggcgctctc tttactgctt
     8641 atcgccgctg ctacctttgt cgttacgctg tttttgccgc ccaatttttg ggtgagcggc
     8701 gtgaaggcga ttgctgaagc ggcgatggtc ggcgcgctgg cggactggtt tgcggtggtg
     8761 gcgctgtttc gccgcgtgcc gattccgatc atttctcgtc atacggcgat tatcccgcgt
     8821 aataaagacc ggattggcga aaatctcggt cagttcgtgc agaaaaaatt tctcgatacc
     8881 caatcgctgg tggcattgat tcgacgtcac gaaccggcgt tgctgattgg caactggttt
     8941 agccagccag aaaacgcccg ccgcgttggt cagcatctgt tgcagatcat gagtggtttt
     9001 cttgaactga ccgatgatgc acgtattcag cgcctgctta agcgcgcggt ccatcgggcg
     9061 attgataagg tcgatctttc cggcaccagt gcgttgatgc tggagagtat gaccaaaaac
     9121 gatcgtcatc aggtgctgct ggatacgctg atcgcacagt tgatcgccct tctccagcgc
     9181 gataaatcgc gcaagtttat cgcccagcag attgttcgct ggctggagag tgagcatcca
     9241 ctgaaagcca aaattctccc caccgaatgg ctgggcgaac atagcgcgga gttggtttct
     9301 gacgcggtga attctttgct tgatgatatc agccgcgatc gtgcgcatca gatccgccat
     9361 gcatttgatc gcgccacttt tgccctgatc gacaagttga aaaacgatcc tgaaatgaca
     9421 gcgcgagctg atgccgtaaa aagctatctg aaagaggatg aagcctttct cagtgaattg
     9481 tggggggatt tacgggaatg gctgaaagcg gatatcaaca gtgaagattc tcgtgtgaaa
     9541 gaacgtatcg cgcgggcagg tcaatggttt ggcgaaacgt taattgccga tgatgccttg
     9601 cgggcgtcgt taaatggtca cctggaacaa gccgcgcacc gcgtcgcgcc tgagttttct
     9661 gcattcctga cgcgccatat cagcgatacg gtaaaaagct gggatgcccg ggatatgtcg
     9721 cggcaaatag agttaaatat cggcaaagat ctgcagttta tccgtgtcaa cggtacgctg
     9781 gttggcggtt gtattgggct aattttatat ttgctgtcac agctcccggc cttgttcccc
     9841 ctcggcaatt tttagaaatc catgaaaaac taagtggcta accgcgagag agatcaaata
     9901 aaccatacta aaagaatggt aataatgtgg cctcttttca ttatctcccg tggtacgggg
     9961 aaggaaaatc atgtcacttg tcaccgatct acccgccatt ttcgatcagt tctctgaagc
    10021 tcgccagaaa ggctttctca ccgtcatgga tctcaaggtg cgcggcattc cgctggttgg
    10081 cacttactgc acctttatgc cgcaagagat cccgatggca gccggtgcgg ttgtggtttc
    10141 gctctgttcc acctctgatg aaaccattga agaagcggag aaagatctgc cgcgcaacct
    10201 ctgcccgctg attaaaagta gctacggctt cggcaaaacc gataaatgcc cctacttcta
    10261 cttttcggat ctggtggtcg gtgaaaccac ctgcgacggc aaaaagaaaa tgtatgaata
    10321 catggcggag tttaagcccg ttcatgtgat gcagctgcca aacagcgtta aagacgatgc
    10381 ctcgcgtgcg ttatggaaag ccgagatgct gcgcttacaa aaagcggtgg aagaacgttt
    10441 tgggcacgaa attagcgaag atgctctgcg cgatgccatt gcgctgaaaa accgcgaacg
    10501 tcgcgcactg gccaattttt atcatcttgg gcagttcaat cctccggcgc ttagcggcag
    10561 cgacattctg aaagtggttt acggcgcaac cttccggttc gataaagagg cgttgatcaa
    10621 tgaactggac gcgatgaccg cccgcattcg tcagcagtgg gaagaaggcc agcgactgga
    10681 cccgcgtccg cgcattttaa tcaccggctg cccgattggc ggcgcagcag agaaagtggt
    10741 gcgcgcgatt gaagagaatg gcggctgggt tgtcggttat gaaaactgca ccggggcgaa
    10801 agcgaccgag caatgcgtgg tagaaacggg cgatgtctac gacgcgctgg cggataaata
    10861 tctggcgatt ggctgctcct gtgtttcgcc gaacgatcag cgcctgaaaa tgctcagcca
    10921 gatggtggaa gaatatcagg tcgatggcgt agttgatgtg attttgcagg cgtgccatac
    10981 ctacgcggtg gaatcgctgg caattaaacg tcatgtgcgt cagcagcaca acattcctta
    11041 tatcgctatt gaaacagact actccacctc ggatgttggg cagctcagta cccgtgtcgc
    11101 ggcctttatt gagatgctgt aaggagtagc agtgacatat tcgattggca ttgattctgg
    11161 ttctaccgcc accaaaggga tcttactcgc agacggcgtg attacgcgcc gtttcctcgt
    11221 tccaacaccc tttcgaccgg caacagcaat tactgaagcc tgggaaactc tgcgcgaagg
    11281 gttagagaca acgccgtttc tgacgctcac cggctacggg cggcaactgg tggattttgc
    11341 cgataaacaa gtgacggaaa tctcctgtca cgggctgggc gcacggtttc ttgcgccagc
    11401 aacgcgcgcg gtaatcgaca tcggtggtca ggacagcaaa gtgattcagc ttgatgatga
    11461 cggtaacctg tgcgatttcc tgatgaatga caaatgcgcg gcgggcaccg ggcgtttcct
    11521 ggaggtgatc tcgcgcacgc ttggcaccag cgtcgagcaa ctcgactgca ttaccgaaaa
    11581 tgtcacgccg cacgccatca cgagtatgtg cacagtgttt gctgaatcag aagtgatcag
    11641 cctgcgctca gcgggcgtcg cgccagaagc gattctcgcc ggagtgatta acgcgatggc
    11701 gcggcggagt gccaatttca ttgctcgtct ctcctgtgaa gcgccgattc tgtttactgg
    11761 tggcgttagt cattgccaga cgtttgcccg gatgctggaa tctcacctgc gaatgccggt
    11821 aaatacccat cctgatgcgc aatttgctgg cacaattggc gcggcggtaa ttggtcaacg
    11881 agtgaggaca cgccgatgaa agagttttta tttttatttc actccacggt cggcgtcata
    11941 caaacccgca aggcgttgca ggcagcgggc atgacttttc gcgtcagcga tattccccgt
    12001 gatttacgcg gcggctgcgg gttatgcatt tggctgacct gtccccccgg cgaggaaata
    12061 caatgggtga tccctgggca aaccgaagcg atatattgcc agcagggcag cgactggcag
    12121 tgcgtggtgc attacgatgc agaagcgtca accaggcaat aggatcttta aatataccaa
    12181 ccggataatt ggctgcgcat aatcctgcat gaaggaagtt gtagtaaata tctcgcctgc
    12241 cgtgaaatat ttcccatcca caaaatgagc gatttttgat agcaccttcc tattgcgcgt
    12301 ctaaaataca atatacattg aattatactt aaacccataa
//
LOCUS       DABSYT020000120        12083 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.120, whole genome shotgun sequence.
ACCESSION   DABSYT020000120 DABSYT020000000
VERSION     DABSYT020000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.120"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            291..1679
                     /gene="narU"
                     /locus_tag="ID725_RS20485"
                     /old_locus_tag="pgaptmp_004097"
     CDS             291..1679
                     /gene="narU"
                     /locus_tag="ID725_RS20485"
                     /old_locus_tag="pgaptmp_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGV7852584.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1761..5501
                     /gene="narZ"
                     /locus_tag="ID725_RS20490"
                     /old_locus_tag="pgaptmp_004098"
     CDS             1761..5501
                     /gene="narZ"
                     /locus_tag="ID725_RS20490"
                     /old_locus_tag="pgaptmp_004098"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGV7852585.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5498..7042
                     /gene="narH"
                     /locus_tag="ID725_RS20495"
                     /old_locus_tag="pgaptmp_004099"
     CDS             5498..7042
                     /gene="narH"
                     /locus_tag="ID725_RS20495"
                     /old_locus_tag="pgaptmp_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGV7852586.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7042..7737
                     /gene="narW"
                     /locus_tag="ID725_RS20500"
                     /old_locus_tag="pgaptmp_004100"
     CDS             7042..7737
                     /gene="narW"
                     /locus_tag="ID725_RS20500"
                     /old_locus_tag="pgaptmp_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGV7852587.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7734..8414
                     /gene="narI"
                     /locus_tag="ID725_RS20505"
                     /old_locus_tag="pgaptmp_004101"
     CDS             7734..8414
                     /gene="narI"
                     /locus_tag="ID725_RS20505"
                     /old_locus_tag="pgaptmp_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGV7852588.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8493..9386
                     /locus_tag="ID725_RS20510"
                     /old_locus_tag="pgaptmp_004102"
     CDS             8493..9386
                     /locus_tag="ID725_RS20510"
                     /old_locus_tag="pgaptmp_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGV7852589.1"
                     /translation="MEPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9482..10326)
                     /gene="nhoA"
                     /locus_tag="ID725_RS20515"
                     /old_locus_tag="pgaptmp_004103"
                     /pseudo
     CDS             complement(9482..10326)
                     /gene="nhoA"
                     /locus_tag="ID725_RS20515"
                     /old_locus_tag="pgaptmp_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10625..11068
                     /locus_tag="ID725_RS20520"
                     /old_locus_tag="pgaptmp_004104"
     CDS             10625..11068
                     /locus_tag="ID725_RS20520"
                     /old_locus_tag="pgaptmp_004104"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGV7852590.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11072..11299)
                     /gene="pptA"
                     /locus_tag="ID725_RS20525"
                     /old_locus_tag="pgaptmp_004105"
     CDS             complement(11072..11299)
                     /gene="pptA"
                     /locus_tag="ID725_RS20525"
                     /old_locus_tag="pgaptmp_004105"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HGV7852591.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11398..>12083)
                     /locus_tag="ID725_RS20530"
                     /old_locus_tag="pgaptmp_004106"
     CDS             complement(11398..>12083)
                     /locus_tag="ID725_RS20530"
                     /old_locus_tag="pgaptmp_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGV7852592.1"
                     /translation="DIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAF
                     EEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQQNSRTAEQQNKRKSQKMTVRYYISSADLTAEKFATAIRNHWHVENKLHW
                     RLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLA
                     SVLAGSGLS"
BASE COUNT         2837 a         3134 c         3124 g         2988 t
ORIGIN      
        1 gtcttgttaa aaatatagtt aatatacaac cctttggatt catcatctga agggttgaat
       61 catgacatgc gtacatagct taaccccgtt taatggttag cttgttagtt acatttagta
      121 acacataact ggctgcttaa tccccattct gcccccgcgg ttacgctttc ttatctattc
      181 tttcaatcag cacttcaccc atttcatcct taattctaat aactggctat tgcgccttac
      241 ctcctgtttt gtcttttttt cattaccaat gtgtacatgt gaggaacaat atggcactgc
      301 aaaatgagaa aaatagtcgt tatcttttac gcgactggaa accagaaaat ccggccttct
      361 ggaaaaataa aggaaaacat attgctcgaa gaaacctctg gatatcagtc agttgtctac
      421 ttcttgcctt ctgtgtctgg atgctattta gcgcagttac cgttaatctc aataaaatcg
      481 gttttaattt cactaccgat caactctttt tattaaccgc attaccctcc gtttctggcg
      541 cattattacg tgtcccctac tcctttatgg tgcctatatt cggtggacgt cgatggacgg
      601 tttttagtac tgcaatcctg attattcctt gcgtctggct cggaattgcc gtgcagaatc
      661 cgaatactcc ttttgggata tttatcgtta tcgctttgct atgcggtttt gcaggtgcga
      721 actttgcttc gagcatgggc aatatcagtt tcttctttcc aaaagccagg caaggtagcg
      781 ctcttgggat taatggcgga ttaggaaact taggtgtaag tgtaatgcag ctggttgcac
      841 cgctggtcat ttttgtaccc gtatttgcct ttctcggcgt caatggagta ccgcaggccg
      901 acggttcggt gatgtcgctg gcgaatgccg catggatttg ggtaccgcta ctagcgattg
      961 ccacgatcgc cgcctggtca gggatgaatg atatcgccag ttcacgcgcg tcaattgccg
     1021 accagctccc tgtcttacaa cgcctgcatc tctggctgct gagcctgctt taccttgcca
     1081 ccttcggttc gtttatcggt ttttctgcgg gtttcgccat gctggcaaaa acccagttcc
     1141 cggatgtgaa tattctgcgc ctggcgttct ttggcccatt tatcggtgcc atcgcgcggt
     1201 cggtgggtgg tgctatttcc gataaattcg gcggcgtgcg ggtgacgttg atcaacttta
     1261 tttttatggc gattttcagc gccctgctgt tccttacctt accgggcaca ggctccggta
     1321 acttcatcgc cttttacgcc gtatttatgg ggctgtttct gaccgcgggt ctgggaagtg
     1381 gttctacttt ccagatgatc gccgtcatct ttcgccagat aaccatttat cggatgaaga
     1441 tgaaaggcgg tagtgatgag caagctcaga gagaagccgt caccgaaacg gcggcggctc
     1501 tgggctttat ctcagccatc ggcgcagtgg gcggcttttt tattccgcag gcatttggca
     1561 tgtcactcaa tatgaccggc tctccagtgg gcgcgatgaa agtgttttta atcttctaca
     1621 tcgtttgtgt gctactgacc tggctggttt atggtcggcg gaagttcagc caaaaataag
     1681 agatcgatgc ctgatgtaca accgtgtcgc atccgacaag gcatcagatc ccaattcatt
     1741 cctggagcag gagtcatgtc atgagtaaac ttttggatcg ctttcgctac ttcaaacaaa
     1801 agggcgatac ctttgccgat ggtcacggac aggtgatgca tagcaaccgc gactgggagg
     1861 acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg ttccacccac ggtgttaact
     1921 gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg tctggtgacc tgggaaatcc
     1981 aacagaccga ctacccgcgc actcgccctg acctgcccaa tcatgaacct cgcggctgcc
     2041 cgcgtggcgc aagttactcc tggtatcttt acagcgctaa ccgcctgaaa tacccgctca
     2101 ttcgtaaacg actgattgaa ctgtggcgcg aagccctcaa acaacacagc gatccggtac
     2161 tggcgtgggc atcgattatg aacgatccgc aaaagagcct gagctacaaa caagtgcgtg
     2221 ggcgcggcgg gtttatccgc tccaactggc cggaactaaa ccagctgatt gccgccgcta
     2281 acgtctggac tatcaaaacc tacggcccgg atcgcgttgc cggtttctcg ccgatcccgg
     2341 cgatgtcgat ggtttcttac gccgccggaa cacgttatct gtcactatta ggtggcacct
     2401 gtttaagctt ctacgactgg tattgcgacc tgccgcccgc ctcgccgatg acctggggcg
     2461 agcaaaccga cgtaccggaa tctgccgact ggtataactc cagttacatc atcgcgtggg
     2521 ggtctaacgt accgcagaca cgtacgccgg acgcccattt ctttaccgaa gtacgctaca
     2581 aaggcactaa aaccatcgcc attacccctg actactctga agtggccaaa ttgtgcgacc
     2641 agtggctggc accaaaacaa ggcactgata gcgccctggc gatggcaatg ggccatgtga
     2701 ttttaaaaga gtttcatctc gataatccca gcgactactt tatcaactac tgccgccgct
     2761 atagcgacat gccgatgctg gtaatgctgg aacctcgcga cgatggtagc tacgtacccg
     2821 ggcgcatgat ccgcgcgtct gacctggtag atggactagg cgaaagcaat aatccgcagt
     2881 ggaaaaccgt ggcggttaat accgcaggtg aattggtagt gccgaatggt tcgattggtt
     2941 tccgctgggg agaaaaaggc aaatggaatc tggaatccat tgccgccggt aaggaaaccg
     3001 aattgtcgtt aaccctgctc ggtcaacatg acgccgttgc aggcgtggct ttcccctact
     3061 ttggcggcat cgaaaatccg cattttcgca gcgtcaaaca caatcctgtc ctggtgcgtc
     3121 aattacccgt taaaaacctg acattagccg gtggcagcac ctgtccagtg gtcagcgttt
     3181 atgatttggt actggcgaat tacggcctcg atcgcgggct ggaagatgaa aacagcgcga
     3241 aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg tgagcaaatt accggcgtgc
     3301 cgcgccagta tattgaaacc atcgctcgtg aatttgccga tactgcccat aaaacgcatg
     3361 ggcgctcgat gattatcctc ggcgcaggtg ttaaccactg gtatcacatg gacatgaact
     3421 accggggaat gatcaatatg ctgatcttct gcggttgtgt cggacaaagc ggtggcggct
     3481 gggcacacta tgtcggtcag gaaaaactgc gcccgcaaac cggctggttg ccgctggcct
     3541 ttgcgctcga ctggaaccga ccaccgcgcc agatgaacag cacctcgttt ttctacaatc
     3601 attccagcca atggcgctat gaaaaagtct ctgcgcagga gttactttca ccgcttgccg
     3661 atgccagtaa gtacagcggt catctgattg attttaacgt tcgcgccgaa cgcatgggct
     3721 ggctaccttc tgcgccgcag ttggggcgta acccgctcgg gattaaagct gaagccgaca
     3781 aggccggatt atcccccaca gaatttaccg cccaggcgct gaaatcgggc gatttacgta
     3841 tggcctgcga acaaccagat agcggcagca atcatccgcg taatttgttt gtctggcgtt
     3901 ctaacctgct tggctcctcc ggcaaaggcc acgagtatat gcagaagtat ctgctgggga
     3961 ccgaaagcgg gattcagggc gaggaactcg gtgccagcga cgggatcaaa ccggaagaag
     4021 tcgagtggca aactgcggcg attgaaggca agctcgacct gctggtgacg ctcgacttcc
     4081 gcatgtccag tacctgcctg ttctccgata tcgttctgcc caccgccacc tggtatgaaa
     4141 aagacgatat gaacacctcg gatatgcatc catttattca tccgctttct gcggcggtcg
     4201 atccggcctg ggaatcacgc agcgactggg aaatctacaa aggtattgcc aaagcatttt
     4261 cgcaagtgtg cgtgggccat cttggcaaag aaaccgacgt ggtattacaa ccactgctgc
     4321 atgactctcc ggcagagctc tcacagccgt gtgaagtgct cgactggcgc aaaggcgaat
     4381 gcgatctgag cccgggtaaa accgcgccga atattgtggc ggtggagcgc gactaccctg
     4441 ctacgtatga acgctttacc tcgctcgggc cattgatgga caaacttggc aacggcggta
     4501 aagggatttc gtggaatacg caggatgaaa tcgatttcct cggtaaactc aattacacca
     4561 agcgtgatgg cccagcgcag gggcgtccgc tgattgacac cgccattgac gcttcagaag
     4621 tgattctggc actggcacca gaaaccaacg gtcatgttgc agttaaagcg tggcaggcgc
     4681 tgggcgagat caccggacgc gaacataccc atctggcgct gcacaaagag gacgagaaga
     4741 ttcgctttcg cgatattcag gcgcagccgc gtaaaattat ctccagcccc acatggtctg
     4801 gtctggaaag cgatcacgtc tcctataatg cgggatacac caacgttcat gagttaattc
     4861 cgtggcgcac gctgtcggga cgccagcagc tctatcagga tcatccgtgg atgcgtgctt
     4921 ttggtgaaag cctggtggca tatcgcccgc ctatcgacac ccgtagcgtc agtgagatgc
     4981 gccagatccc gccaaacggc ttcccggaaa aagcacttaa cttcctgacg ccgcaccaga
     5041 aatggggcat tcactcaacc tacagtgaaa acctgctaat gctgacgctc tctcgcggtg
     5101 gaccgattgt ctggatcagc gaaaccgatg cccgtgaact aaccattgtc gataacgact
     5161 gggtggaagt gtttaacgcc aatggcgcgc tgacggcccg cgcggtggtc agccaacgtg
     5221 taccgccggg tatgaccatg atgtatcacg ctcaggaacg cattatgaat attcctggtt
     5281 cggaagtaac tggcatgcgc ggcggtattc ataactcggt cacccgcatt tgcccgaaac
     5341 caacgcatat gattggcggt tacgcgcagc tggcctgggg ctttaactac tacggcaccg
     5401 tcggctcgaa ccgcgacgaa ttcatcatga tccgcaagat gaagaacgtt aactggctgg
     5461 atgatgaagg tcgcgatcag gtacaggagg cgaaaaaatg aaaatccgtt cgcaagtcgg
     5521 catggtgctt aatctcgata aatgtatcgg ctgccatacc tgttcggtga cctgtaaaaa
     5581 cgtctggacc ggacgcgaag gcatggagta cgcatggttt aacaacgtcg aaaccaaacc
     5641 gggcattggt tatccgaaaa actgggaaga tcaggaagag tggcaaggcg gctgggtccg
     5701 cgatgtgaat ggcaagatac gcccgcgtct gggcagcaag atgggtgtaa taaccaaaat
     5761 cttcgccaac ccggtggtgc cgcagattga tgattactac gaacctttca ccttcgacta
     5821 cgaacatttg catagcgcac cggaaggcaa acatatccct actgctcgcc cgcgttcgct
     5881 gattgacggt aagcggatgg acaaagtgat ctgggggcca aactgggaag aactgctggg
     5941 cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag gccatgcaaa aggagatgta
     6001 cgggcagttt gaaaacacct tcatgatgta cctgccgcgc ctgtgcgaac actgcctcaa
     6061 tcccagctgc gtggcgacct gcccaagcgg cgctatctac aaacgcgaag aagacggcat
     6121 tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg tgcattagcg gttgtccgta
     6181 caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa aaatgtatct tctgttaccc
     6241 acgaattgag tccggtcaac cgaccgtgtg ctcagaaacc tgcgtgggtc gcatccgata
     6301 tctgggcgtg ctgctttacg acgctgaccg catcgaggaa gcggcgagca ccgagcgcga
     6361 ggttgacctc tatgaacgcc agtgcgaagt gttcctcgat ccgcacgatc cctcagtgat
     6421 cgaggaagcc ctgaaacaag gtattccaca aaatgtgatt gacgctgccc agcgttcgcc
     6481 agtctacaaa atggcgatgg actggaaact ggcactaccg ctgcaccctg aataccgcac
     6541 cctgccgatg gtctggtacg ttcctccgct gtcaccgatt cagtcctacg cagatgcggg
     6601 cggtttgccg aaaagcgaag gcgtgctgcc cgccatcgaa agcctgcgta ttccggtgca
     6661 atatctcgcc aatatgttga gtgccggcga taccggtccg gtactgcggg cgctgaaacg
     6721 gatgatggcg atgcgccact atatgcgttc acaaaccgtg gaaggcgtta ctgatactcg
     6781 tgccatcgac gaagtaggcc tgagcatcgc ccaggtcgaa gaaatgtatc gctatctcgc
     6841 cattgccaac tatgaagatc gttttgttat cccgaccagc catcgggaaa tggcgggcga
     6901 tgccttcgca gaacgcaatg gctgcggttt tacctttggt gacggttgtc acggctcgga
     6961 cagcaaattc aacctgttca acagtagccg tatcgatgcc atcaacatca ccgaagtgcg
     7021 cgacaaagcg gagggcgaat aatgcagatc ctcaaagtga tcggcctgtt gatggagtat
     7081 ccggacgaga tgttgtggga atgcaaggag gacgcgctgg cgttgatccg ccgcgatgcg
     7141 ccgatgctta cggatttcac ccgcaacctg cttaacgcgc cgctgctgga taaacaggcc
     7201 gaatggtgcg aagtgtttga ccgcgggcgc accacgtcgc tgctgctgtt cgaacatgtt
     7261 catgccgagt cccgcgatcg cggccaggca atggtggacc tgctggcgga gtatgaaaag
     7321 gtcggcctgc agctggattg tcgggaactg cccgattatc taccgctgta tctggagtat
     7381 ttaagcgtac tgcctgacga tcaggcgaaa gaaggattgc tcaacgttgc gccgatcctc
     7441 gccctgcttg gcggtcgctt aaaacaacgc gaggcaccgt ggtacgcgtt gtttgatgct
     7501 ctgctgcaac tggcgggaag tacactttca agtgacagtg tcacaaaaca agtaaacagc
     7561 gaagagcgcg atgacacccg ccaggcgctt gatgcagtgt gggaagagga acaggtgaag
     7621 tttattgaag ataacgccac ggcgtgtgac agctcgccgc ttaatcaata tcagcgacgc
     7681 tttagtcagg atgtcgcgcc gcaatatgtc gacatcagtg cgggaggtgg gaaatgattc
     7741 agtatctgaa cgtctttttt tacgatatct acccgtacat ttgtgcgacg gtgtttttcc
     7801 tcggcagctg gctgcgctac gactacgggc agtacacctg gcgcgcctcc tcaagtcaga
     7861 tgctcgataa acgcgggatg gtgatatggt cgaatctgtt ccatatcggc attttgggga
     7921 tcttcttcgg gcacctgttc ggcatgttaa cgccacactg gatgtacgcg tggtttttgc
     7981 cagtggcagc gaaacaacta atggcgatgg tgctcggtgg tatttgcggt gtgttgacgt
     8041 tgattggcgg cgcaggactg ctgtggcgca ggctgacaaa tcagcgcgtg cgtgccactt
     8101 ccactacgcc ggatatcatc atcatgagca ttttgctgat ccagtgtctg ttggggctaa
     8161 gtaccatacc gttctcagca cagtatcctg atggtagcga aatgatgaag ctggtgggtt
     8221 gggcgcaaag cattgtcact ttccgtggtg gatcgtcaga gatgcttaac ggtgtagcgt
     8281 tcgtcttccg cctgcatctg gtgttgggaa tgaccatctt cttgctcttc ccattcaccc
     8341 gactggtgca tgtgtggagc gccccgtttg agtactttac tcgtcggtat cagattgtgc
     8401 gctcgcggcg ataatttcac cctggccgga tagcccttcg ctctccggcc acttgcaggc
     8461 tatgatagtt ctccagtcac aggagaacgt taatggaacc gcaggtttac cacgtcgatg
     8521 cttttacctc tcaaccattt cgcggcaatt ctgccggtgt tgttttcccc gccgataatc
     8581 ttagcgaagc gcaaatgcag cttatcgccc gcgaattagg tcattcggaa accgcttttc
     8641 tgctgcacag cgacgacagc gatgtgcgca tccgctactt tacgccaaca gttgaagtgc
     8701 ctatttgcgg tcacgcgaca gtagctgcgc actatgtacg tgcgaaggtg ttaggtttag
     8761 gaaattgcac ggtctggcaa acatcgctgg caggaaaaca tcgcgtgact atcgaaaagc
     8821 accacgatgg ttatcgtatt tcgctggaac aaggtacgcc aggctttgag ccaccgctgg
     8881 aaggtgaaac acgtgcggcg attatcaacg cgcttcatct cactgaggac gatattctgc
     8941 caggcttgcc aattcaggtg gcaaccacag gacactcaaa agtgatgatc ccactgaaac
     9001 cggaagtgga tatcgacgcc ctttcgcctg accttaatgc gctgaccgct atcagcaaac
     9061 agattggctg caatggtttc ttcccgttcc agatccgccc aggcaaaaac gaaaccgatg
     9121 gtcgcatgtt ctcgcctgcg attggtattg tggaagatcc ggtgactggc aatgccaatg
     9181 gcccgatggg tgcatggttg gtacatcaca acgtattgcc ccacgatggc aacgtgttgc
     9241 gtgttaaagg ccatcagggg cgcgcattgg ggcgtgacgg tatgattgaa gtgacggtga
     9301 caattcgtga taaccaaccg gaaaaagtca ccatttctgg cgcggcggtg attttgtttc
     9361 atgccgaatg ggcaattaaa ctctgaaacc gttgcctgat gcgcttcgcc tgtcaggtct
     9421 acaaccgggc taatgtatgc cggataaggc gtttacgccg catccggcaa cctgacataa
     9481 attattttcc cgcctccggg tgcgtatcaa acgccgccat caccagcgct aactcatcca
     9541 cggtaaagcc atgtttcgca tcatccacgc ccagaccaaa ctgttcttgc atcacagcat
     9601 ataacgacgc cacatccggt agatttcgct gctccaccgc gtgcccattt tcataatggg
     9661 aaaatgaaaa ttggtcagtg tcagcttgcc gccgtccggc aaatggcggc acatcagcaa
     9721 atgatggcga aaatgcgact gcggccagtg cgccgaccag aaattaccca tcacataatc
     9781 gctttgttgc tgctcgcaga gatcaaaacg gtacatcgac tgccagtgct gatgatgatt
     9841 aaactgcaac acccaatcat caccctcctg caacaaccga tactctccgt gtggcgtggt
     9901 ctgcacaaga tcgggaacta aacgaatcgg cgcggttagc gtctgcccac cgaaaccgac
     9961 atcagcaatc catttttcct cttccagttc caccaacagc aaacgatggg tgcgcggcgg
    10021 taatgctggc ggatttgata acactacccg ccctaacaag ctgcgaacgt taaaccccag
    10081 ctcgcgtaac acccgctcaa aaacgccatt ctgctcaaaa cagtaaccgc cacgacgggc
    10141 tatcaccagt ttctcttcca gcgattgatc atcaagctgc atttccctcg gcagcaaaac
    10201 gtcgaggttt tcaaacggaa tggtgcaatt gtgtttcagg tgcaatgcac gaagcgtatc
    10261 aatattgacc gcagcagctc ccgaccagtt aatacgggca aaatagtgat tcagaatggg
    10321 cgtcatcagg cgtttccctt agtggttttc tcaacttata acctggaata gacgccgcac
    10381 cggctatttt gtgcgatcgc ggacatgcat cacgttcgca aatcgccaat cccgttaatt
    10441 tcgttactta ttgcgttacg gactacgctt aaaaaaacct tcttcgcgag gcgataccat
    10501 gagccgattc atccccatag aattacacca tgccagccgg ctgttaaatc atggcccaac
    10561 tgtcatgatc accagtttcg atgagcaatc ccagcggcgt aacattatgg ctgcagcctg
    10621 atcaatgccg gtggagtttg aacctccacg cgtgacgatt gtggtagata aatcgacatg
    10681 gaccagggag ttgattgaac gtaacggtaa atttggcatc gttatcccgg gcgttgcagc
    10741 aactaactgg acgtgggcag tgggaagtgt gtcgggtcgt gaagaagata aatttaattg
    10801 ctatggcatt ccggttgtga gaggcccggt atttggtttg cctctggtcg aggaaaaatg
    10861 tctggcgtgg atggagtgtc gattgctacc tgcgacttct gcgcaagaag aatacgacac
    10921 gctgtttggc gaagtagtat cagcagcggc agacgcacgg gtatttgtcg aaggccgctg
    10981 gcagtttgat gatgataagc tcaatacgtt gcatcattta ggtgctggga cgtttgttac
    11041 cagcggcaag cgtgttacgg cgggttaagc attacatgct gtaaccgggt ttctttatca
    11101 aagcctccat ttggggcgcg atttcggcat cccagatagc ttgccaagat tctggttgaa
    11161 tctgttgtag agcaatgctt atcgaactgt ctttactgtt cagatggcga ataataacgt
    11221 cggtaatatc tgcagcaagt gctgcttttt gttgttcgtc cagttcacgc ggaaaacatt
    11281 taatgtctat gtgcggcatg attacgctcc ttttgaaaga tgtcagtaaa attaacacac
    11341 attgtgaatg tcgtgtgtcc ggtaaagcgt ttttacgaca ataatgccag ggcaagatta
    11401 cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg
    11461 catcttacgt cttaaccctg ccttgaatac cttatcattc gtcaaaatat taatagcaat
    11521 gtgccgtatc cctgaaaata attctgctgc atttcctctt cttattttgc agtcgtcttc
    11581 attcattacc acgtccagac gccagtgcag cttattctcc acgtgccagt ggtttcggat
    11641 tgctgtggcg aacttctctg cggttaaatc agcagaactg atataatatc tgaccgtcat
    11701 tttttggctc tttcttttgt tctgctgttc tgctgttctg ctgttctgct gttctgctat
    11761 tatcgaccga aaggagactg ccacgcataa tttcttcagc cctttccatt caaacgtgaa
    11821 atcaataagt tcatcaggga catcgcaaac aatatgaaga cggatttctt ctctgccgtg
    11881 actcttttca ctaattgcgt aactgtcatg ctctggatta tttaattctt tcagcggaaa
    11941 tttttcctca aaggctttat ttagccgccc ctggtttcct tttaccgcga ataaataatc
    12001 acctccctgt ttttgtatct tctctgcaat atctttctgg caacccatcg catcagttgt
    12061 gatgattttt cctttaatat cca
//
LOCUS       DABSYT020000121        12041 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.121, whole genome shotgun sequence.
ACCESSION   DABSYT020000121 DABSYT020000000
VERSION     DABSYT020000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.121"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(1..137)
                     /locus_tag="ID725_RS20535"
                     /old_locus_tag="pgaptmp_004107"
                     /pseudo
     CDS             complement(1..137)
                     /locus_tag="ID725_RS20535"
                     /old_locus_tag="pgaptmp_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            287..955
                     /locus_tag="ID725_RS20540"
                     /old_locus_tag="pgaptmp_004108"
     CDS             287..955
                     /locus_tag="ID725_RS20540"
                     /old_locus_tag="pgaptmp_004108"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGV7852593.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1453..1635
                     /locus_tag="ID725_RS20545"
                     /old_locus_tag="pgaptmp_004109"
     CDS             1453..1635
                     /locus_tag="ID725_RS20545"
                     /old_locus_tag="pgaptmp_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGV7852594.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
     gene            1714..2214
                     /locus_tag="ID725_RS20550"
                     /old_locus_tag="pgaptmp_004110"
     CDS             1714..2214
                     /locus_tag="ID725_RS20550"
                     /old_locus_tag="pgaptmp_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGV7852595.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            2251..2757
                     /locus_tag="ID725_RS20555"
                     /old_locus_tag="pgaptmp_004111"
     CDS             2251..2757
                     /locus_tag="ID725_RS20555"
                     /old_locus_tag="pgaptmp_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGV7852596.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            2776..3666
                     /locus_tag="ID725_RS20560"
                     /old_locus_tag="pgaptmp_004112"
     CDS             2776..3666
                     /locus_tag="ID725_RS20560"
                     /old_locus_tag="pgaptmp_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGV7852597.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(3786..4367)
                     /locus_tag="ID725_RS20565"
                     /old_locus_tag="pgaptmp_004113"
     CDS             complement(3786..4367)
                     /locus_tag="ID725_RS20565"
                     /old_locus_tag="pgaptmp_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGV7852598.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(4367..7282)
                     /locus_tag="ID725_RS20570"
                     /old_locus_tag="pgaptmp_004114"
     CDS             complement(4367..7282)
                     /locus_tag="ID725_RS20570"
                     /old_locus_tag="pgaptmp_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGV7852599.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAVSQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSSDITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHS
                     VSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(7347..7946)
                     /locus_tag="ID725_RS20575"
                     /old_locus_tag="pgaptmp_004115"
     CDS             complement(7347..7946)
                     /locus_tag="ID725_RS20575"
                     /old_locus_tag="pgaptmp_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGV7852600.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(8013..11411)
                     /locus_tag="ID725_RS20580"
                     /old_locus_tag="pgaptmp_004116"
     CDS             complement(8013..11411)
                     /locus_tag="ID725_RS20580"
                     /old_locus_tag="pgaptmp_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGV7852601.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(11472..>12041)
                     /locus_tag="ID725_RS20585"
                     /old_locus_tag="pgaptmp_004117"
     CDS             complement(11472..>12041)
                     /locus_tag="ID725_RS20585"
                     /old_locus_tag="pgaptmp_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGV7852602.1"
                     /translation="KTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQL
                     HETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMT
                     GILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYG
                     EMRVGSRVVSQEISTADEGDGGQVVVIGR"
BASE COUNT         2897 a         3191 c         2848 g         3105 t
ORIGIN      
        1 gctactgatg tatcaacacg attcaacaat acccgatatt tattccatgc ctccagcaac
       61 gatctttctt cctccgttgc gatttccaga tctacagcat cctgcagtgg cgcaatatac
      121 tcactgaatt cctggatgta gaactgtgtg gtgacggtct tccagccatt cggctcctgc
      181 tgtatcgcag cataccaggc tatttcaata tcgctatgct gcggcagcat ttaacccctt
      241 gtaattcatc gccataattg atttaattca caaataaaac tataacatgg tgaaattaat
      301 gaaaaaaaac acagatgatg gggctaaaat ttacacacca cttaccctaa agctttatga
      361 ctggtgggtt ttgggagtat caaatcgtct tgcatgggga tgtcctacaa aggaacacct
      421 tcttccacac tttctggaac atttaggtaa caaccatctg gatattggtg ttggaactgg
      481 gttttacctt actcacgtac ctgagagtag tctgatatct ttaatggatt tgaacgaagc
      541 tagcctgaac gcggcatcta caagggctgg ggaatcaaaa attaaacata aaattagcca
      601 tgatgttttt gaaccttatc ccgcggcgtt acatggtcaa tttgattcca tttccatgtt
      661 ttaccttctt cactgcctgc ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc
      721 ggcgcaggcc ttaactgacg atggaactct atacggagcc acaattcttg gcgatggagt
      781 tgtgcacaat agcttcggtc aaaaactgat gcgcatttac aatcagaaag gcatcttttc
      841 aaacacaaaa gattccgaag aaggcttaac acatatactc tcagagcatt tcgagaatgt
      901 taaaaccaag gttcaaggta ctgtagtaat gttttccgct tcagggaaaa aatagcatcc
      961 aaccgcagca cgttcttgca tacgccgtgt ctgcggttta atttttactc tcaaatattg
     1021 tagttaagta caataatgat tcctgtggct atatagatgt ttttgtaatt agtccttaag
     1081 ataaaatttc tctatttatc caagaaacag acttcattag gaagagtcgt aaaaacatta
     1141 acaactaccg ccgtgtaatt aaattatttt ttagaatgtt ctgattgaga aaaacaacgc
     1201 catacacatc aaaagatata gaaatgtaaa tttagattta ttaatctttt gagttacaac
     1261 ctaaactttc cgttaatcac caagatatca acagattata ctcactgagt acacattttt
     1321 acaattgcta tttctcacat taagatgcgc ataccaatct cttatttgac actttcttat
     1381 ttgttactag agttaattgg gtgctgacaa ggcacatgtt atttacaatt tgaaaatcag
     1441 aggagaaatg atatgaccga acatcgtggc ggtgcaggta atttcgctga agatcgacaa
     1501 aaagcttctg acgctggtcg taaaggcggc caacatagtg gcggtaattt taaaaacgat
     1561 ccacaaagag catcagaagc agggaaaaaa ggtggtcaga atagccatgg tggcggccgt
     1621 aaatctgact cttaatttat caccccctac attcgtgaat aactgaagct aactgcgagc
     1681 ctacgggctc gcagtgaaaa ctctggagtg attatgcaaa ttaaatctgt tgaagatatc
     1741 ttcatccact tattatcgga tacatacagc gctgagaaac aactaacaaa ggcactgtct
     1801 aaacttagtc gctctgcata tagtgataaa ttaaccgctg cttttcagtc acatcttgat
     1861 gagacacatg gtcaaattga acgcatcgat caggttgttg attctgaaga tggtcttaaa
     1921 ttgaaaagaa ttaagtgtgc cgcaatggaa ggacttatcg aagaggcgaa tgaggtaatt
     1981 gaaagcactg ataaaaatga agtacgtgat gctgcattga ttgctgcagc acaaaaagta
     2041 gagcactatg aaattgccag ttatggcact ttggtaacac tcgctgagca attaggatat
     2101 aaaaaagcgg ctaaactcct gaaagaaaca ctggaagagg aaaaagctac tgatgtcaaa
     2161 ttaacagacc tggcatttaa taacgttaat aaaaaagctc aggataactc ctgaactata
     2221 gacttcaatt tcttttagga taaacagatt atgaatagaa ttgaacatta ccatgactgg
     2281 cttcgtgatg cacatgcgat ggaaaaacaa gctgaaaaaa tgcttgagtc tatggccagc
     2341 cggatagaaa actatcctga gttacgttcc agaattgagc agcacattag cgaaacaaag
     2401 aaccagcttt cacagcttga aagcatactt gatcgtaata atatttcgcg ctctgtcatt
     2461 aaagactcta tgagtaaaat ggccgcgttt ggacaatcca ttggtggaat ttttccatct
     2521 gatgaaattg tcaaaggttc gatcagtggc tatgtgttcg aacaatttga aattgcgtgt
     2581 tacacatctc ttctcgcagc agccaaaaat gcgggtgata cagcttcagt acccattatt
     2641 gaagcaattc ttaatgaaga aaaacaaatg gctgaatggc ttcttaacca tattcctgac
     2701 accactgagc aattcatggt cagatctgaa attgatggag ttgaagccaa aaaataattc
     2761 ttttgaggag gcattatgtt tagacacgtg aaaaaacttc aatatacagt acgtgtaagt
     2821 gagccaaacc caggcttggc taatctactc ttagaacaat tcggtgggcc acagggtgag
     2881 cttgcagcag catgccgata cttcactcaa ggcttaagtg atgaagatgc aggccgaagg
     2941 gatatgctga tggacatagc aacagaagaa ctaagtcatc tcgaaattat tggttctctt
     3001 gttggtatgc tcaacaaagg tgcaaaagga gcacttgctg aaggaactga gaatgaagct
     3061 gagctttacc gttccttaac tcaaaatggc aatgatagcc atataacttc tctcctctac
     3121 ggaggaggcc cagcattaac taattcagct ggagtaccct ggactgcagc ttatgttgat
     3181 actattggcg aagtcacggc cgatcttcgt tcaaatattg cagctgaagc tcgggcaaag
     3241 atcatttacg aacgtctcat taatgttact gatgatcctg gagtaaaaga cactctggca
     3301 tttttaatga cccgggaagc agcacacatg ctttcatttg aaaaagcatt acattcaatt
     3361 cgtaacacct ttccaccagg aaaattacca ccacttgaga aatacaaaaa cgtatattac
     3421 aacatgtcag aaggtgaaga cgtcagaggt agttggaaca gtgatgaaaa ctttgactat
     3481 gtttccgatc cagtttcagc ggttgacggt ggggatggga aggcctcgat aaatctttcg
     3541 accaagcaag aagctatgat aaaagctatg gcaacaaggt tgaaatccca tgaagacatc
     3601 aatcctgtca ctggcgctga gcttgctgaa ggagaaccac aaacaaagat aaatagtaaa
     3661 aactaattat cttctggtat ttagctatcc ctcataagtt aattgccgct gagatatcct
     3721 aagctctagc ctctgaaaat acatttatat ttcgaagccc gcgatatttt aagcgggctt
     3781 ttattttaaa cttcaggtat tacaggccac tcaatatcag gtgcagttga tgtatcaaca
     3841 cggttcagca acacccgata ctttttccag gcttccagca atgaggtttc ttcctccgtt
     3901 gcgatttcca gatctacagc atcctgaagt ggcgcaatat gctcactggc tacctgcatc
     3961 aggctgtttt ttgtttcttc cgcctcccgg atccggaaca gtttttctgc ttctgcatct
     4021 ttcacccagg ctgtgccgtt ccacttctga aactcccctt ccggcgataa ccaggtaaca
     4081 ttttccggta atgagccgag ttcagaaata aataacgcgt cgccggaagc cacgtcataa
     4141 accgttttac cccgatgatc ttcaacgaga tgccacgatg actcatcact gttgaaaaca
     4201 gccacgaagc cagccggaat atctggcggt gcaatatagg tactgtttgc tggcagacct
     4261 gtatgaggcg gaatatatgc gtcaccttca ccaataaatt cattagttcc ggtcagcaga
     4321 ttataaattt ttatggtccg tggttgttca ctcattctga atgccattat gcaagcctca
     4381 caatgtagtt aaatgcgatg tttttgacag tgttttccgc gttacccgca gcgttaacgg
     4441 tgatggtgtg tccatgtgag ccaatcgcaa cagagtgcgt atgagcacca ataccgacag
     4501 tatgtgcgtg agcacctgca gatgcagctg tgccgctgac actatgagtg tgcgctcctg
     4561 cagcacttgt attcacactg gccgcagaca ctaccttatg tactgaccca ttttgtgacc
     4621 actggctgac tgctgatgcg cctgtgacac tatgagtatg gtttccggca cttgcggctg
     4681 taccgctcac actgtgcgtg tgcgccccgg tgttattcgt ggatttagtg ccgtaatcaa
     4741 acgacgatgt ggttttcgtc cccaaatccg tactggatgc gctggcgctg tgggtgtgcg
     4801 atttaatgcc gtcctgttcc tgagacaata cggcccgacc actggcaggt ttgcccttaa
     4861 tcgtccagcc acgcatatca gggatcacgc ctgacggata agcggctgca agtttcgggt
     4921 atgcagattt gtcaaaagtc tgcccctgca tcagggcata gccagacgga acggtatctg
     4981 atggccacgg gattggtgcg ccaggcggat aaaactgctc tgatggcgta tagagtgaat
     5041 aaactgtacc gtccgttaac ccttccggct tattagcaga atatgctggt gacgtatgaa
     5101 tcgtcacgct ggcattactg gtataatccc attgaatatt tacaccagtc gcataatttc
     5161 cgattgcaac gtaaatatcg taagtatcac cagatgtatt gacccaggca aaatttgtaa
     5221 accctgtcga tgtgcgctgc cataaagcac cagtaatccc cttcggatta ccattacctg
     5281 cacgcaaaac aagttcagat atacctgcct gttgaggtga ccccacgtta aatcccgcgc
     5341 caccaatcaa cgtaattgaa acaacagaac tcgcctgtgg catggttacc gttgctaatt
     5401 tgaaccaacc agcaccaccg ctgaatgaca ttgttgttga gttaagcgta ccaatatctt
     5461 tcggcgtcag tgttatatcc gatgaaagcg ccttaccatt caccttacgg gcagaaggta
     5521 cccgaccatt cgcattatca ttagctgctt tcactgcttt cggtgtcgcg gcaagcgttt
     5581 cagatgcgct gttggttgca ctactgagct ggacaattcc tttttgtgct gtcgtagcgt
     5641 cctgagcagt atatttcccg ttagcaaggt cataggctgc ctttaccgac tttggcgttg
     5701 ccgccagcat ttcagatgtg ctgttggtcg cgttgctaag ctgaactatc cctttctgtg
     5761 ctgtcgttgc atcctgtgcg gtgtatttcc cgttagccag ctcatacgcg gccttaacgg
     5821 cttttggcgt tgccgccagt gactcggaag tgctgttagt cgcactgctg agctgtacta
     5881 tccccttttt cgtcgtgctc gcatcctcaa gcgccacggc ggatgcaata tcctctgccc
     5941 gttttgccgc tgtctcagcg cgcgttgctg cagattccgc cgtacttttg ctctgagctg
     6001 ctgccgtcgc actaccagct gcctctgtcg ccttcgtgga tgccgtcgtg gcgctgctct
     6061 tcgctgctga cgcttgtctg gtcgcctcat cttttgaagc agacgcagat gatgccgatg
     6121 acgccgccga actggctgac gatgcggcag ccgtttttga ggattctgcg ctggtttccg
     6181 acgctttcgc gttcgtttcg gatgtcttcg ctgcggaagc agaactcgct gctgcgctgg
     6241 cctgttcagt ggcttcgcca gccttcgttg tggctgttga agcagacgat gcggcacttt
     6301 ctgccgattt tccggcggcg gtggcactgg ctgaggcctg cccggcactt gttgacgcgg
     6361 cactggcaga taatgcagcc gctgtttttg agcctgctgc tgcggaggca ctctgttctg
     6421 ctgccgtttc agaggactta gcgtttgtct cagacgtttt ggccgccttc gcggaattgc
     6481 ctgccgccgt tgccgaggag gctgcactac tggcgctcga ggctgcgctc gtttctgatg
     6541 attttgccgc ctcttttgaa gccgacgcat ccctggctga ggaggcagct tctgacgctt
     6601 tcgtggtcgc ggtggatgca gaagtggcgg ctgattgttg tgacactgcc gcattcgttt
     6661 ctgacgtttt cgccgcaccg gcactggtag ccgccgcgct ttttgaggac tctgcagcgg
     6721 cagcactttt tgatgcttca gtagcctttg ttgatgccgt tcctgcgctg gaagacgctg
     6781 actgagccga cgacgcggcc tgtccggctg acgtgctggc tgcgcgtgct gagtccgcag
     6841 catcagtcgc atgggttgcc gcctcacggg ctgatgtgct ggcatcgctg gctgacttct
     6901 tcgcggctgc cgtgttctgt gccaccgcgg acgcgttacg cgccacctct tccaccatca
     6961 gctcaaaacg gcgcagtgcc tccggacgga catcatcctc agtcatggca ccaagaaaat
     7021 cattcagcgt accgggttga gaatcttcat acacggtgat ggtcccggca tgtgacggcg
     7081 ggaacccttc caccaacaga ataacgctgt actgaccgta ctcaacgtcc atgctgtaac
     7141 gcccggcttc atccggattt tctgaggcca gcgtgttcac caccaccgtg gtactgttac
     7201 gttttgcttt cagctggatt gtgcagttct gtaccggttt tcctgtgccg tctttcagta
     7261 cacctgaaat ctttactgcc atattcaccc cacaaaaaag cccgcctgaa ccggcgggct
     7321 gtcataacac tgtgttacct ggctaatcag aatttataac caacacccac gatgaacccg
     7381 tcagtgcgcc agtcaccact gccagaacct tcataagcaa tatcaatggc cacggattcg
     7441 gtcgggttaa actgcacgcc agccccccac gccagagacg tgttgctgtg gcgaccgtca
     7501 tcacttccgg tcagcacatc gtgcgttttc cccttgttgt cagttacgcg aaggtaatcc
     7561 ccggaaaaag tcgaaacacg gctgtaagcc atacccgcca tcgcatacgc gctgaaccat
     7621 tcattcacgc gcacagacgg ccccgccatc acgctgaacc agcggttacg cacggaatct
     7681 tcatgccagc gggtatcgct gtaacgggtc agctggcgat tcttgtctcc tgcatagctg
     7741 aatgacgtca ccagccccag cgtatccgta aactcataac ggtatttcac gttaatcccg
     7801 ttcagatcat cactgccggg aacgttggtc cgggcatgaa gataccccgc gctcagcgtg
     7861 gactgatgtt cagatgccca tgcaggcgca ccggatacgg ccagacaaat ggctgcggac
     7921 aaaatggcgg cataaagttt acgcataatt acctctcgct tttctgcaat aaaaaaggcg
     7981 tcatttctga cgcccgttct gggttataaa attcagctga tactgatacc tgctgtggat
     8041 tttttcatca ccacaaccag cagatcgctg atactggttg ttggtgtcca gttattcgct
     8101 cctgatgaag atacggtgaa tgtcagtgtc agcgtcccct gtccggcagg catatctata
     8161 actgaggaaa atacgccctg agcatccgtc gtggactgat taaaaatctc ctggccattg
     8221 cgggtcactc ttaaccggca ggttgaatac cagtatgact gttggttatt actgttgaaa
     8281 ttctcatgct taccaccgcg gaataacact ggcggtatca tgacctgccg gtcaaatttc
     8341 tgatcatcac tgattcttac cgtgatggtg ccactggcat aagtgctcgt gcgggggaaa
     8401 gacttgctga ccgttttgac aatatcgcct tcaatctgat tggctgacag tttcccctta
     8461 atctgacagt tctcattaat cgtgacgttg ttgagcgtcc cggagttcgc attcacactg
     8521 ccactgatat ccgcattttt agcagtcagc tttccgtccg gtgtcaggga aaatgccggt
     8581 ggatttccac cgctggtaat ggtgggggcc gtcaggcgct tcaggaacac gtcgttcatg
     8641 aatatctggt tgccctgcgc cacaaacatc ggcgtttcat tcccgtttgc cgggtcaata
     8701 aacgcgatac gattggcggc aaccagaaac tggctcagct tgccttcctc cgtgtcctcc
     8761 atgctgaggc caatacccgc gacataatgt ttgccgtctt tggtctgctc aattttgacg
     8821 ccccacatgg cattccactt atcgttagca tccttccact ctttcgaaaa ctcatccagt
     8881 ctgctggcgt tatcctccgt caggtcaact ttttccagta gctctttacc aagatgagat
     8941 tcggttatct tgcctttgaa aaaatccaga taaccttccg catcatcgct cgcccgaccg
     9001 acggcctcca cgaatgccga tttgccaacg gtgttcacac tgcggatata aaagtaataa
     9061 tcatggcccg gtttgatatt gatactggcg gctatccagt acagcgccgt accaagataa
     9121 cgcgcgctgg tttcaacctg cctgatatcc gcaatccgcg tttccgagaa ccagaactca
     9181 aactgtaccg tcggatcata aaccgcaaga tgcggcgtgg cggttatctg aaaatagcct
     9241 ggtgtcagct caatccgaga cggcgctgcc ggtgcggcaa tccggaacga taccgatgcc
     9301 ggatcgccct gctgtcccca cgcatttacc gcccggacag tcagcctgta gttccccagc
     9361 gccagttgcg tgaagcggta tgtggtttcc gtcgtcctgg ccgtactgac cagccgctca
     9421 ctgccatcgt ccgctgccac ggtcaggcga agcaggaagc tcaccccctt caccaccttc
     9481 ggcgtgtccc agcgcgccag cacctgatat tccccgctgt ctgcggtgac ttcggcagtc
     9541 aggtgctgca ccgcgggcgg cgtgacgcca ttcaccgtgc cgctcaggtc gccgtcaaag
     9601 tgcgccccgt tatccacgat ggcttctttt tcgggtacat gctgcacggc ggtgatggca
     9661 tacgtgccgt cgtcgttctc acggatactc acgcagcgga acaggcgctg gcgcaacgtc
     9721 ggcaacttca gcccccatac gctgtatccg gcaacgccgt caggaacacg gctcactttt
     9781 accttcacgc cgtcggtgac ggactggacc tccacgctga ccggattgcc acttccgtca
     9841 accaggctta tcagcgtggt accggaggat ggcagcgtga tttcacggtc gagcgtcagc
     9901 gtccgggtct ggctgttcac cgccagcaca cgcccaccga tgctgatccc cgcatagtca
     9961 tcatcacaga tttcaatgac atcgcccggt acatggcgaa gcccttctgc gcccacgctg
    10021 aaatccacgg tctgcgtctc cagcagctcc gttttaatca gccacagccc ggcgcggtgt
    10081 gcctgccccc ggctggtaca gccaaaggca tccatcttcg tgacgttacg accgtaacgg
    10141 agaatggcct gcgtgtcctc cacaagctct gtcgccgtct cccagccgtt atccgggtca
    10201 atccagttca cctcaacggc attatggcgg tccttgaggg cgctgaagct gtagcggaac
    10261 gacgcgccat catccggcat caccacatta ctgcggttat aggtccacac cttatccgac
    10321 ggtcggtcct gcacgaacgt cagcgtctgc ccgttccata ccggcataca gcgcatcgcc
    10381 gagcagaaat cgctgagaac atcccacgcc ttacgctgtg tggtcaggta ggcattacag
    10441 gtgatgcgcg gctccgtgcc gccaaagccg tccggcactg actggtcgca gtactggccg
    10501 atgacataca gcgcccattt atccacatcc gccgcaccaa gacgtttccc catgccgtag
    10561 cgcggatggg tcagcatatc ccacagacac caggccatgt tgttgctgta tgccggttta
    10621 aacgttccgt cccagatacc gctgtattgc cgcgtctgcg ggttataatt cgacggcacc
    10681 tgcagaatac gcccgcgcag atgataatta cggctcacct tctggctgcc gaactgctcc
    10741 gagtccacct gcacgccgac cagtgccgtg ttcgggtagc actgtttcac atcgataatt
    10801 tcggtgtatg acgaccagag cgttttgttc tgcagctggt ctgtggtgct gtccggcgtc
    10861 atcctgcgca tccggatatt aaacgggcgc ggcggcaggt tatccaccac caccgaggcc
    10921 agatactgtg aagtggtttt acccttaatg gtgatgtctt tttccgtcac ccagccaccg
    10981 ttacgttgta tctgaaccag caggcggact tccgatggat tcctgtcccc ctttgaggtg
    11041 gtttccacca gtgcctgcac gccgaaagta aaacgcagac ggtcaatgtt tgccgacgtg
    11101 atggtccggg tgatcggcgt gtcatatttc acttctgtac cgagcaccgt ctcggagccg
    11161 gaggattcaa atccctccgg cggtgtctgc tcctgctcac cggcccggaa caccaccgtg
    11221 acgccggata tattggtatt cccctcactg tccagcaccg gcgtactgtt cagcagcacg
    11281 ctttttaatc catccaccgg accttcaacc ggcccttcgc tgatggcatc gatcacactc
    11341 agcagctgcg tggacttcag gttgtccttc gcttcgcgcg gggtatgccc cttactgctg
    11401 cctttaccca ttcgtcatgc tccataaacg ataaaaccgc ccggaggcgg tttcacataa
    11461 aacattttgc atcagcgacc aatcaccaca acctgaccac cgtccccttc gtctgccgtg
    11521 ctgatctctt gagaaaccac gcgtgacccc acgcgcattt caccgtacag aaccggcaga
    11581 acattgccct gggcaaccat gttatccagt gaggagaaat aggtgttctg tttgccgtta
    11641 tccgttgtct gtgtacgggg agttctggct ttcggtgcca gcatctgcgc cacaccaccg
    11701 agcaccatac tggcaccgag agaaaacagg atgccggtca taccaccggc cccaatggct
    11761 gccccccatg ctgcaagggt ggctccggcg gtaaagaatg atccggcaat ggcggcagcc
    11821 cccaggacaa tctggaatac gccacctgac ttggccccgg cgactctggg aacaatatga
    11881 atcacagcgc catcaggcag agtctcatgt aactgcgccg ttaacccgga cgtgctgacg
    11941 tcccgcccgg taatccgtac ctgataccag ccgtcgctca gcttctgacg aaacaccggg
    12001 agctgtgtgg ccagtgcgcg gatggcttca gcccccgttt t
//
LOCUS       DABSYT020000122        11965 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.122, whole genome shotgun sequence.
ACCESSION   DABSYT020000122 DABSYT020000000
VERSION     DABSYT020000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.122"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            157..489
                     /gene="hdeA"
                     /locus_tag="ID725_RS20590"
                     /old_locus_tag="pgaptmp_004118"
     CDS             157..489
                     /gene="hdeA"
                     /locus_tag="ID725_RS20590"
                     /old_locus_tag="pgaptmp_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGV7852603.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            605..931
                     /gene="hdeB"
                     /locus_tag="ID725_RS20595"
                     /old_locus_tag="pgaptmp_004119"
     CDS             605..931
                     /gene="hdeB"
                     /locus_tag="ID725_RS20595"
                     /old_locus_tag="pgaptmp_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGV7852604.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            995..1642
                     /gene="yhiD"
                     /locus_tag="ID725_RS20600"
                     /old_locus_tag="pgaptmp_004120"
     CDS             995..1642
                     /gene="yhiD"
                     /locus_tag="ID725_RS20600"
                     /old_locus_tag="pgaptmp_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGV7852605.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1694..2464)
                     /locus_tag="ID725_RS20605"
                     /old_locus_tag="pgaptmp_004121"
     CDS             complement(1694..2464)
                     /locus_tag="ID725_RS20605"
                     /old_locus_tag="pgaptmp_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HGV7852606.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(2461..3453)
                     /gene="shuU"
                     /locus_tag="ID725_RS20610"
                     /old_locus_tag="pgaptmp_004122"
                     /gene_synonym="chuU"
     CDS             complement(2461..3453)
                     /gene="shuU"
                     /locus_tag="ID725_RS20610"
                     /old_locus_tag="pgaptmp_004122"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HGV7852607.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEPQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(3502..4125)
                     /gene="chuY"
                     /locus_tag="ID725_RS20615"
                     /old_locus_tag="pgaptmp_004123"
     CDS             complement(3502..4125)
                     /gene="chuY"
                     /locus_tag="ID725_RS20615"
                     /old_locus_tag="pgaptmp_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HGV7852608.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(4125..4619)
                     /gene="hutX"
                     /locus_tag="ID725_RS20620"
                     /old_locus_tag="pgaptmp_004124"
     CDS             complement(4125..4619)
                     /gene="hutX"
                     /locus_tag="ID725_RS20620"
                     /old_locus_tag="pgaptmp_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HGV7852609.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(4632..5969)
                     /gene="hutW"
                     /locus_tag="ID725_RS20625"
                     /old_locus_tag="pgaptmp_004125"
     CDS             complement(4632..5969)
                     /gene="hutW"
                     /locus_tag="ID725_RS20625"
                     /old_locus_tag="pgaptmp_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HGV7852610.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            complement(5989..6903)
                     /gene="shuT"
                     /locus_tag="ID725_RS20630"
                     /old_locus_tag="pgaptmp_004126"
     CDS             complement(5989..6903)
                     /gene="shuT"
                     /locus_tag="ID725_RS20630"
                     /old_locus_tag="pgaptmp_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HGV7852611.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            7586..9568
                     /gene="chuA"
                     /locus_tag="ID725_RS20635"
                     /old_locus_tag="pgaptmp_004127"
                     /gene_synonym="shuA"
     CDS             7586..9568
                     /gene="chuA"
                     /locus_tag="ID725_RS20635"
                     /old_locus_tag="pgaptmp_004127"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HGV7852612.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            9617..10645
                     /gene="chuS"
                     /locus_tag="ID725_RS20640"
                     /old_locus_tag="pgaptmp_004128"
     CDS             9617..10645
                     /gene="chuS"
                     /locus_tag="ID725_RS20640"
                     /old_locus_tag="pgaptmp_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HGV7852613.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(10706..11236)
                     /gene="dctR"
                     /locus_tag="ID725_RS20645"
                     /old_locus_tag="pgaptmp_004129"
     CDS             complement(10706..11236)
                     /gene="dctR"
                     /locus_tag="ID725_RS20645"
                     /old_locus_tag="pgaptmp_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGV7852614.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11392..11958)
                     /gene="slp"
                     /locus_tag="ID725_RS20650"
                     /old_locus_tag="pgaptmp_004130"
     CDS             complement(11392..11958)
                     /gene="slp"
                     /locus_tag="ID725_RS20650"
                     /old_locus_tag="pgaptmp_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGV7852615.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3114 a         2800 c         2968 g         3083 t
ORIGIN      
        1 gcagtatctg atgcatctgt aactcattgt attgaaataa aaatatctga ttttgatatt
       61 ttccatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga aattgattcg
      121 tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt aggcgttatt
      181 cttggtggtc tgcttcttct gccagttgtg agcaatgcag cggatgcgca aaaagcagct
      241 gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt ggacgaatcc
      301 ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa accagaagat
      361 gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca ggcttgtact
      421 caggataaac aagccaactt taaagataaa gttaaaggcg aatgggacaa aattaagaaa
      481 gatatgtaat tccgggaatg cgttacatcg tacttccttg catattgaac aggccggaat
      541 atcttcttta aaagcagcta ttcctcctgt tcatatataa tctctatatt gaatgggtta
      601 caaaataaat atttcatctc tccgtaaagc gtttattttt atgggcgctg tagcggcttt
      661 gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag atatgacctg
      721 ccaggaattt attgatctga atccaaaagc aatgaccccg gttgcatggt ggatgctgca
      781 tgaagaaaca gtatataaag gtggcgatac agttacttta aatgaaaccg atctcactca
      841 aattcctaaa gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt ataccttcaa
      901 aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg agtggcaagt
      961 tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta ttatacgtct
     1021 tatactcgcg gcaattgcct gtggcgctat tggcatggaa aggcaaatgc gcggcaaagg
     1081 agcagggtta cgcactcatg tattaattgg catgggaagc gcactgttta tgattgtttc
     1141 gaaatatggt tttgctgacg tgctgtcttt agatcacgtc ggactggacc ccagccgtat
     1201 tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc tcgttcgtaa
     1261 ccaaaatatt gtaggtttga cgacggcagc ggatatctgg gtgaccgccg ccataggtat
     1321 ggttattggc agtggtatgt acgaactggg tatttatggt tcagtgatga ccttgctggt
     1381 gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc atttacagct
     1441 gacattagtg aacggtaata cggtatcaat gctcgactgg tttaagcagc aaaaaataaa
     1501 aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca ttgatatcca
     1561 gcttcatgcc acaacgtcga tagaagatct gttgcgatta ttaaaaggta tggcaggggt
     1621 gaaaggcgtt tcaattagct aatctgtata gttatttatt cgcaggagta ccagtgcggt
     1681 actcctgttg cttttaatga tcgaggacga tcagtggtga atggttggca ggatctttaa
     1741 gtacggtaat atccgcgccg tagagcattg tcagcgcctg ctgtgtcaat acgtcctgtg
     1801 gtttaccatt tgcgataacc tttccttttt gcattaatac aacacggtct gcataatgcg
     1861 cggcaagatt tagatcgtgc aatatgcagc agacattgaa ctgccgctcg tgaactaact
     1921 ggcgtaataa acgaaacaag tgctgctgat ggtgaatatc cagcgccgaa gttggttcat
     1981 caagaaataa ccatttcggt gagggagttg gttcccagag ttgcaccagc aggcgcgcta
     2041 attgcacgcg ctgttgttca ccaccggata attgccgata gtcgcggttt gctaacgcct
     2101 gacagtcaca taacgccatt atttgtgctg tttcattatc ctgattacca gtacgatgcg
     2161 gatgccgacc catctggata acttcttgta cgctgaatgg aaacgccatg tggctatttt
     2221 gacgcatcac ggcccggtgt ttcgccagtt cagtaatcga ccattcattt aatggcttat
     2281 taaacaaccg acattcgccg ctatcaggtt gaagatagcc ggttaactgg cgaagcagcg
     2341 tagattttcc cgcaccatta gggccgagaa tggcaacaat ttccccaccg ggaaaagtca
     2401 gcgagacatt atcagtcaga cgccgcccct gaaggctata aaccagattc tgggcgctga
     2461 tcatgtgatc ctccgatttc gcagaatcaa ccacataaaa taagggccac caagcaggct
     2521 ggttaataat cccacgggca tttctgctgg ctgtaccagc gtgcgggcaa gcgtgtctgc
     2581 catcagcaat aaacaggctc cggcgagggc ggaacaaggg atgagccagc ggtgatttgc
     2641 tccggtagtc atgcgaatca gatgcgggat caccaggcca ataaagccga taatgccgct
     2701 taccgatacg gcagcaccaa cgagcagtga gctcactaat agtaattgct ggcgtttacg
     2761 cttaacgttc acgccgaggt aatgggcttc ttcatcaccg agctgcagta aattcagcgt
     2821 tccggcgaga caagttgttg cgataatggc cggaaggatg aatgaactgg caaccaataa
     2881 cgtcggccat tgcgcctgtc caagattgcc catcatccac aatgtgagtt gtcgtagctg
     2941 cggctcatcg ccaatatagc tcaggatgcc gatcgctgcg ccgcacaggg cattgatggc
     3001 gataccggcg agtaataatt gcatcatgct gccatcgcga tggcgctgcg tgatgagaaa
     3061 aatgatggcg cagactacta aacttccggc aatggcaaac accatttgct catagagcac
     3121 cagcaccact gggaaggaga agggcagcac aattgcaacg ccaaccatta atgcagaacc
     3181 gctactgacg ccgagtaatc ctgggtcagc cattgggttg cgaaaaagcc cctgcataat
     3241 tacccctgcg gttgccaacg ctgcacctac cagcaccgcc agcaaaaccc gtggtagacg
     3301 aatattgagc cagatatcgc ggtattcctc atcaaaaagg gcatcaaaac tgatctttaa
     3361 cgctccctga ctagcaccga acagcaccag ggcaagcaat aatagtgata gccccataaa
     3421 gaccctggcg gatgaaaatg aatccttgag cacgcaaaac tccgttacga cattacggcg
     3481 cggacatgcc gcgccggggt atcacgccgg tttcaggtcc ggttcaatca gactgtagac
     3541 ctgctggtta agtgctggcg catttgccag ttcatggata tgtgcggcga catcggcacg
     3601 gcggacaaaa ccatgacact cctgattttg tatccgttgt gctttgcctg ttgctgcgcc
     3661 gtcaagcaga ccaccagggc ggagaatggc gtagtctagc tggctggttt gcagccagct
     3721 ttccgccagc gttttttccc gtaccgcctg accaaacgca gcctttgccc gctccgataa
     3781 aaacggccag ctatcgccgc atcccaaaga ggtcaccaga atcatgcgac tgatgccggc
     3841 cttttcggcc tcatcaatca ctgttctgtg cgccagataa tcctgagctc ctcccattgt
     3901 tgagatgata agcgcatccg ggccagctgc gcggcaagca gccgcgacta cgctggcatc
     3961 acaggcgtcg cctgtaaaaa cctgtacgcc ttgttgcgcc agtttcgtgg cggcatcggc
     4021 atgacgaatg acagcaacga ccggacgttg ttccgccagc gccagttcaa gcgttctggc
     4081 acccacacct tttccgcctg cgccaaagag tagccacggc gtcatcaggc gtgttccttt
     4141 aacgatgccg ccagagtatg gaaggcgctg acttgttcgc tcaggagctg acgatggtca
     4201 tccctgccga ggaagatttt cagcatggcg ctgccttctt tgttgaaaaa gagaatggac
     4261 gcggtgtcca tgcccataaa tttacgttcg attaaggcaa tatgcgtgca gttttctgct
     4321 ttgatatgcc cggacatgcc gtgctttccg cgtaggttga aatagccgtg gcgatggaaa
     4381 cccgaaggca gttcgccgct aaattcgagg attacatcgg cagtatgtac taacgtggtg
     4441 acgttgcccc attcgcagac ggtatcccag acagtatcga atttatcgcc tggcactacg
     4501 gtagaggagg gtagatttct cactacctcc agcagtgtgg tgttgtattg ttcggctacc
     4561 acctccagcg taccgtccgg ctccgttttc aaaaattctt gtaacgagac atggctcata
     4621 aatcactcct gttatggttt ttcacgcata atcgctggcg cattaagtac ctgaattagt
     4681 tcattaagag actgcaaaat attgcttgcc cagaaacgac cttcattagt cagccgcagg
     4741 caagttgaag catcgcgact taagcctttt tggtgccatt gagccagtaa cggcgcgagt
     4801 ttttcggcat gtggcgtaag ttcatccagc ggtacgcgcg ctgtttcaac acctgactgc
     4861 aaggtatgac gccattgcgc attacgttcg gcgttacgca tgatcaacat cagcggtttt
     4921 ttgcctgccg cgacggattc atgccaggtc tgaagattgc gttcgttcat ccaggagtaa
     4981 ccattaatcg aaccgccggc tccggaacca aaggccagac aatcggcacc ttgtttaatc
     5041 agcaggttat agagattgcg ttcgcgtgtg gtacggcccc agtggctgtt actgatgcag
     5101 cgccaaccgg catcgtccat aaaatcacat ccttgcagat aaagatcgcg acgttctgcc
     5161 ggtgagggta cggtagtacg cccattttcc acggccttgc ccagcggtgt attggacagg
     5221 acattgagcg catagagatc gacgccgtcg agaccgatat cgcgggcaat agccagatct
     5281 tctccccagg tttgcgcatc ctgacccggc aggccaaaca gcaggtcaca gaccaccgca
     5341 gcacggtcac gtttgaccag gctttccata aacgcaatag cggttgggcc atccgaggtg
     5401 cgggccattt tcttgcggat tttgctgtta aacgactgga taccaattga gaagcggttt
     5461 gctcctgcat caaggcaagc atcgattcgc tcagcgtcaa aattcaatac ccgtccttcg
     5521 atagtgattt cacaatcagg ggccagtggc agcttttctc gtaatgtggt gatgattctg
     5581 gccaaatcgt gtgccgaaag tgcggaaggc gtaccgccac cgaaatagac cgcgtggatg
     5641 ggggcagact gatgcaatac gctgtccgct tccatttcta tttcacgaat cagggcgtcg
     5701 gtataatgag cacacgcatc ttcattaaaa cggttctgat aaaaaccaca aaacgtgcag
     5761 tgtgtcgcac aaaaaggaat gtgtaggtaa accagtcgct tacgaggtga tgccgtttga
     5821 ttgatcatct cttgccaggt ctgtgccagt tgctctttgg caaccggaat ggctccacga
     5881 aacggcatca tggcgcgcct gtctttaaat ggctgatcac cgtctaaagc aaaatgtggc
     5941 gtgagatcca gggtgttatt tgcgttcatt cgtctgattt aaatccattt attgcggcca
     6001 aagctgctgg tgtaaagact caacgacatc agcaatacgc ggacccattc ccagaatcag
     6061 gttttgatca acggtgataa tgcgctggtt tttccatgcg gcagtgtggg taattccagc
     6121 aatagaacgt agccgattaa tatcgccatc gaccatttgt gaggttacga caatcacttc
     6181 tggattagcc gcaatcagtg attctgcact gtagcttttg tactgctggt gggtagcgac
     6241 gttttctgct ccggcaagcg aaagaatggc gtccgcgacg ctgcctttac cggcaacttg
     6301 tggcgcactt ccgcctgcgg agagaataaa catggcttta accggggctt ttttggtcgc
     6361 cacgttttgc tgtactcgct ccaggcgttg gctgatttgt gtcaccagcg cctcgccttg
     6421 ttcaggaacc tgtagcgttt ttgccagttg gcgaatgttg gcgtacatct gctgaagggt
     6481 ggcaggcaca cgcggcaggg tgacgacgtt gactttctgc gctcgtagtt ggtcgagcac
     6541 aatttgtggc ccggcatcct gccaggtaat tacgctatcc gggcgaagtg acaaaatgcc
     6601 ttcgctgctg agttgtttcc agtagccaat atgcggcagt ttggcggttt ctggtggata
     6661 agatgtcgtt tcatcgacac caaccacgcg ttcgccagcg cccatcgcgt agattagctc
     6721 cgtcagcgat cctcctgcga ccacgatacg ttcggcagcc gttacgctaa aagcccagct
     6781 aagagccagt actgccgtaa acaatttttt acgttttact gcggtcttcg cggcagaggc
     6841 cacggctgaa atacataacg tcagtgggga aaaaagaaac ggcctggtga tgatccttgg
     6901 cataatatga gaactcttat caaattgaga atgatcatga ttatcatttg agttatatat
     6961 gaaatacaac cgtcgattca atacgcaagg cgttacaagc gtatttattt ttgattttta
     7021 tcaagatgct tcgtcagcta acgttcttgt ttccgactaa atttcatcgc gggaaaaagc
     7081 tgagaaccca gagatatcga ggcttgcaga gcaaagagaa gaaaaaatta tcactgttaa
     7141 cgacaaaatt tagtgatgag gcatgggggg ataaccataa aaacaaggtg gtttacaggg
     7201 cgcaggatag gtttcataac cccctgttta ttgtgttaat ggtggttaaa aaaaaacttg
     7261 atcataaaaa attgatcgat aataattctc attatcacgt ttgcatttaa caaaacacga
     7321 ccatatggat aacaaaacca tagcatttgc aaggggtaac gcacagctct cttcactcct
     7381 gatggggcat atcgttttgc ctgacaaaga gggcgtgctt ttgcgtaatt gccgagttat
     7441 caggcaattt catgggatat aaacgcttgt aaactcaccc ggcgaatatt atcgctctga
     7501 attttaaata aatttatttt tcgccagcca ccccggtatt aacccaggca gccagcattt
     7561 tttattttcc tcatggagaa tcgctatgtc acgtccgcaa tttacctcgt tgcgtttgag
     7621 tttattggcc ttagctgttt ctgccacctt gccaacgttt gcttttgcta ctgaaaccat
     7681 gaccgttacg gcaacgggga atgcccgtag ttccttcgaa gcgcctatga tggtcagcgt
     7741 catcgacact tccgctcctg aaaatcaaac ggctacttca gccaccgatc tgctgcgtca
     7801 tgttcctgga attactctgg atggtaccgg acgaaccaac ggtcaggatg taaatatgcg
     7861 tggctatgat catcgcggcg tgctggttct tgtcgatggt gttcgtcagg gaacggatac
     7921 cggacacctg aatggcactt ttctcgatcc ggcgctgatc aagcgtgttg agattgttcg
     7981 tggaccttca gcattactgt atggcagtgg cgcgctgggt ggagtgatct cctacgatac
     8041 ggtcgatgca aaagatttat tgcaggaagg acaaagcagt ggttttcgtg tctttggtac
     8101 tggcggcacg ggggaccata gcctgggatt aggcgcgagc gcgtttgggc gaactgaaaa
     8161 tctggatggt attgtggcct ggtccagtcg cgatcggggt gatttacgcc agagcaatgg
     8221 tgaaaccgcg ccgaatgacg agtccattaa taacatgctg gcgaaaggga cctggcaaat
     8281 tgattcagcc cagtctctga gcggtttagt gcgttactac aacaacgacg cgcgtgaacc
     8341 aaaaaatccg cagaccgttg aggcttctga aagcagcaac ccgatggttg atcgttcaac
     8401 aattcaacgc gatgcgcagc tttcttataa actcgccccg cagggcaacg actggttaaa
     8461 tgcagatgca aaaatttatt ggtcggaagt ccgtattaat gcgcaaaaca cggggagttc
     8521 cggcgagtat cgtgaacaga taacaaaagg agccaggctg gagaaccgtt ccactctctt
     8581 tgccgacagt ttcgcttctc acttactgac atatggcggt gagtattatc gtcaggaaca
     8641 acatccgggc ggcgcgacga cgggcttccc gcaagcaaaa atcgatttta gctccggctg
     8701 gctacaggat gagatcacct tacgcgatct gccgattacc ctgcttggcg gaacccgcta
     8761 tgacagttat cgcggtagca gtgacggtta caaagatgtt gatgccgaca aatggtcatc
     8821 tcgtgcgggg atgactatca atccgactaa ctggctgatg ttatttggct catatgccca
     8881 ggcattccgc gccccgacga tgggcgaaat gtataacgat tctaagcact tctcgattgg
     8941 tcgcttctat accaactatt gggtgccaaa cccgaactta cgtccggaaa ctaacgaaac
     9001 tcaggagtac ggttttgggc tgcgttttga tgacctgatg ttgtccaatg atgctctgga
     9061 atttaaagcc agctactttg ataccaaagc gaaggattac atctccacga ccgtcgattt
     9121 cgcggcggcg acgactatgt cgtataacgt cccgaacgcc aaaatctggg gctgggatgt
     9181 gatgacgaaa tataccactg atctgtttag ccttgatgtg gcctataacc gtacccgcgg
     9241 caaagacacc gataccggcg aatacatctc cagcattaac ccggatactg ttaccagcac
     9301 tctgaatatt ccgatcgctc acagtggctt ctctgttggg tgggttggta cgtttgccga
     9361 tcgctcaaca catatcagca gcagttacag caaacaacca ggctatggcg tgaatgattt
     9421 ctacgtcagt tatcaaggac aacaggcgct caaaggtatg accactactt tggtgttggg
     9481 taacgctttc gacaaagagt actggtcgcc gcaaggcatc ccacaggatg gtcgtaacgg
     9541 aaaaattttc gtgagttatc aatggtaatc atctgccccg atatttcggg gcatttatct
     9601 ggaaggaaga gagacaatga accactacac acgctggctt gagttaaaag aacaaaatcc
     9661 cggaaagtac gcgcgtgaca tcgcagggtt aatgaatatt agagaagcag aactggcatt
     9721 tgcacgcgtc acgcacgatg cgtggcggat gcacggcgat atccgtgaaa ttctggcggc
     9781 gctcgaaagt gttggcgaaa ccaaatgtat ttgtcgtaat gaatatgcag tccatgagca
     9841 agttggtacg ttcacaaacc agcatttgaa cggacatgcc ggattgatcc tcaatccgcg
     9901 cgcgctggat ttacgtctgt ttctcaatca atgggccagt gttttccaca tcaaagaaaa
     9961 cacggctcgt ggcgaacgcc agagtattca gttctttgat catcagggcg atgcattact
    10021 aaaagtttat gccaccgaca ataccgatat ggcggcatgg agtgagcttc tggcacggtt
    10081 tatcaccgat gagaatacgc cgcttgagtt aaaagccgtt gatgcgccag ttgttcaaac
    10141 gcgagccgat gccactgtgg tcgagcaaga gtggcgggcg atgaccgacg ttcatcagtt
    10201 ttttacgttg ctcaagcgcc acaacctgac gcgccaacag gcgttcaatc tggtggcaga
    10261 cgatttggcc tgcaaagtat ccaacagtgc gttggcgcaa attcttgaat ctgcacagca
    10321 ggatggtaat gaaatcatgg tgtttgttgg caaccgtggc tgcgtacaga ttttcaccgg
    10381 tgtggtagaa aaagtggtgc caatgaaagg ttggctgaat attttcaacc cgacgtttac
    10441 tcttcatcta ttagaagaga gcattgctga agcctgggtt acccgtaaac cgaccagcga
    10501 tggctacgta accagtctgg aattgtttgc ccatgatggt acgcagatag cgcaacttta
    10561 tggtcaacgt acagaaggcg aacaggagca agcgcaatgg cgtaagcaaa ttgcttcgct
    10621 gataccggaa ggcgttgctg cataacgtat aattaacggg aggtctggct atttattgct
    10681 gggcctcttt atattgatca atcgttcaca ccagataatc aatatgctga tggcgtacca
    10741 gctcattgat ccgcttaaga ttgaggatca tcatgatatt gtgtttatgg cagtagaacg
    10801 tttttagcga tattttcagt ttctccagaa tatcttccgt tgtcatttga ttcgcaatgc
    10861 agctcagaac ctgatgttgc ttgcgcgaaa gtgacacatt cgcttcaggc acagagcagt
    10921 aactgccatt gatgagtgcg acaaaatcaa tgatagtgat agtccgcgga acaaaggtca
    10981 ccggcacttt ccccaggcag cgtttaatat taaaggggga gaaaataatg acatggacag
    11041 gttttaatcg ttcaatttga gtgagaaaat tagaatgaaa tacattattc attaatgtat
    11101 caataatgac gaaggcattc tgatgcaaca ttacgtcgat ctcttcttcg ttctgtatat
    11161 gaacgacatt acctttactc agaatgtttt tcatcgcggt gaagaacatc gtatccctgg
    11221 taattataag aaacataacc gactcctggt gcggacatta actttataat cagtttgcca
    11281 aaggtaatta acaggacagg gcttataata ataatagtta gcttactgtg tattaatttg
    11341 atgtaatgca tgatgcagat gctgagaaag catctgcatc tttcggtggt gttatttgac
    11401 cagctcaggt gttacctgac tcaccgcatt ggtgtagtaa ggcgcacccc agcccggttc
    11461 cggccagaat gcaccatagc cgtaatccca caggttatag gtggtattaa ccacttctct
    11521 caaatgccac acctggatgc cctgcatatt cacttccagg aagttatacg ggactttatt
    11581 gataaagcca ggttgttcac cctgaatggt gccgaggatg gtaacaaagt gattacgata
    11641 gttcactgga tcaaggaagc cgctttgtct ggcgagcagt cggccctgat agttggcttc
    11701 aatatcaggc ttcgcatagc tatccaacgg taatacagcg atttctaaca acgtatccgt
    11761 tttgccattg ataacgttga taaccttccc accaaagcgc gcttgttgac caacatataa
    11821 ccccggctgg ttatgaacag caacaaaact tttttgaata tcaggttggt tatttccttt
    11881 gatattttgc ggaattgaac tacatgctgc aagcaaaaat gaaaggctga gtatgagtgc
    11941 accttttgtc atgttcatgt tacta
//
LOCUS       DABSYT020000123        11958 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.123, whole genome shotgun sequence.
ACCESSION   DABSYT020000123 DABSYT020000000
VERSION     DABSYT020000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.123"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            66..1118
                     /gene="ybhH"
                     /locus_tag="ID725_RS20655"
                     /old_locus_tag="pgaptmp_004131"
     CDS             66..1118
                     /gene="ybhH"
                     /locus_tag="ID725_RS20655"
                     /old_locus_tag="pgaptmp_004131"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGV7852616.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1194..2627
                     /gene="ybhI"
                     /locus_tag="ID725_RS20660"
                     /old_locus_tag="pgaptmp_004132"
     CDS             1194..2627
                     /gene="ybhI"
                     /locus_tag="ID725_RS20660"
                     /old_locus_tag="pgaptmp_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGV7852617.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2810..5071
                     /gene="ybhJ"
                     /locus_tag="ID725_RS20665"
                     /old_locus_tag="pgaptmp_004133"
     CDS             2810..5071
                     /gene="ybhJ"
                     /locus_tag="ID725_RS20665"
                     /old_locus_tag="pgaptmp_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGV7852618.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGSSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5212..6495)
                     /gene="ybhC"
                     /locus_tag="ID725_RS20670"
                     /old_locus_tag="pgaptmp_004134"
     CDS             complement(5212..6495)
                     /gene="ybhC"
                     /locus_tag="ID725_RS20670"
                     /old_locus_tag="pgaptmp_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGV7852619.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6630..7700)
                     /locus_tag="ID725_RS20675"
                     /old_locus_tag="pgaptmp_004135"
     CDS             complement(6630..7700)
                     /locus_tag="ID725_RS20675"
                     /old_locus_tag="pgaptmp_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7852620.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(7678..7896)
                     /locus_tag="ID725_RS20680"
                     /old_locus_tag="pgaptmp_004136"
     CDS             complement(7678..7896)
                     /locus_tag="ID725_RS20680"
                     /old_locus_tag="pgaptmp_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGV7852621.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(7936..8103)
                     /locus_tag="ID725_RS20685"
                     /old_locus_tag="pgaptmp_004137"
     CDS             complement(7936..8103)
                     /locus_tag="ID725_RS20685"
                     /old_locus_tag="pgaptmp_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852622.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            8346..8948
                     /locus_tag="ID725_RS20690"
                     /old_locus_tag="pgaptmp_004138"
     CDS             8346..8948
                     /locus_tag="ID725_RS20690"
                     /old_locus_tag="pgaptmp_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852623.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(9159..9380)
                     /locus_tag="ID725_RS20695"
                     /old_locus_tag="pgaptmp_004139"
     CDS             complement(9159..9380)
                     /locus_tag="ID725_RS20695"
                     /old_locus_tag="pgaptmp_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGV7852624.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(9479..9694)
                     /locus_tag="ID725_RS20700"
                     /old_locus_tag="pgaptmp_004140"
     CDS             complement(9479..9694)
                     /locus_tag="ID725_RS20700"
                     /old_locus_tag="pgaptmp_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGV7852625.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(9771..9962)
                     /locus_tag="ID725_RS20705"
                     /old_locus_tag="pgaptmp_004141"
     CDS             complement(9771..9962)
                     /locus_tag="ID725_RS20705"
                     /old_locus_tag="pgaptmp_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGV7852626.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(9935..10117)
                     /locus_tag="ID725_RS20710"
                     /old_locus_tag="pgaptmp_004142"
     CDS             complement(9935..10117)
                     /locus_tag="ID725_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGV7852627.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            complement(10114..10794)
                     /locus_tag="ID725_RS20715"
                     /old_locus_tag="pgaptmp_004143"
     CDS             complement(10114..10794)
                     /locus_tag="ID725_RS20715"
                     /old_locus_tag="pgaptmp_004143"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGV7852628.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(10791..11462)
                     /gene="bet"
                     /locus_tag="ID725_RS20720"
                     /old_locus_tag="pgaptmp_004144"
                     /pseudo
     CDS             complement(10791..11462)
                     /gene="bet"
                     /locus_tag="ID725_RS20720"
                     /old_locus_tag="pgaptmp_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            11492..11674
                     /locus_tag="ID725_RS20725"
                     /old_locus_tag="pgaptmp_004145"
     CDS             11492..11674
                     /locus_tag="ID725_RS20725"
                     /old_locus_tag="pgaptmp_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGV7852629.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            11671..11841
                     /locus_tag="ID725_RS20730"
                     /old_locus_tag="pgaptmp_004146"
     CDS             11671..11841
                     /locus_tag="ID725_RS20730"
                     /old_locus_tag="pgaptmp_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGV7852630.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            11831..>11958
                     /locus_tag="ID725_RS20735"
                     /old_locus_tag="pgaptmp_004147"
     CDS             11831..>11958
                     /locus_tag="ID725_RS20735"
                     /old_locus_tag="pgaptmp_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGV7852631.1"
                     /translation="MMAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERR"
BASE COUNT         2868 a         2848 c         2958 g         3284 t
ORIGIN      
        1 gagatgcttt gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca
       61 ttattatgaa aaaaataccc tgcgtgatga tgcgaggtgg aacctcgagg ggcgcgttcc
      121 tgttagcgga acatttaccc gaagatcaaa cgcagcgcga taaaatattg atggcaatta
      181 tgggttccgg taacgatctg gaaattgacg gtattggcgg cggtaatcca ctgaccagta
      241 aagtcgccat tattagccgt tccagcgatc cgcgtgctga tgttgattat ctgtttgctc
      301 aagttatcgt ccatgagaaa cgtgtcgata ccacgcctaa ctgcggcaat atgctgtctg
      361 gcgttggggc attcgccatt gaaaatggtt tgattgcagc gacttcgcca gttacccgcg
      421 tacgtatccg caacgtcaat acgggtacgt tcatcgaagc tgatgtgcaa acgccaaatg
      481 gtgttgtcga gtacgagggt agcgccagaa ttgacggcgt accgggtact gccgcaccgg
      541 ttgcgctcac tttcctgaat gccgctggaa cgaaaaccgg aaaagttttc ccgactgata
      601 atcagattga ttattttgac gatgtcccgg tgacctgtat cgatatggcg atgccagtcg
      661 tcattattcc ggctgaatat ctgggaaaaa caggctatga attaccggcg gaactggatg
      721 ccgacaaagc attattagcc cgcattgaat ctatccgtct acaagcgggt aaagcaatgg
      781 gcttaggcga tgtcagtaat atggttatcc ctaaacctgt gcttatttct ccagcgcaga
      841 aaggcggggc aattaatgtg cgttatttta tgccgcattc ttgccatcgc gcgctggcga
      901 taaccggtgc tattgctatt tccagtagtt gtgcattgga aggtaccgtc acccgacaaa
      961 tcgtcccttc tgtaggatac ggcaatatca atattgaaca ccccagtggt gggctcgacg
     1021 ttcatttaag taatgaaggt caggatgcca cgacgttacg cgcatctgtt attcggacaa
     1081 ccagaaaaat attttccggt gaagtttatc ttccctgaaa agattcgttg tcaggataag
     1141 gacaatcaat aaaggacttc tgtatgagtc atacagaaag aacaggattt taaatgaata
     1201 agaaatcgtt atggaagcta attctgatat tagcgatccc atgtattatt ggctttatgc
     1261 cagctccggc aggattaagc gaactggcgt gggtgctttt tggtatttac ctggcggcca
     1321 ttgtggggct ggttatcaag cctttcccgg aacctgtcgt actgttaatt gccgttgctg
     1381 cctccatggt ggtggtcggt aacttatccg acggtgcgtt taagaccacc gccgtattaa
     1441 gcggttactc ttcaggtact acctggctgg tgttctcggc gtttacctta agcgccgcat
     1501 ttgtgaccac cggtttaggt aaacgtattg cctatctgct gattggtaaa atcggtaaca
     1561 ccacgctggg tctgggttac gttacggtat tcctcgatct ggtattggct ccggcaacac
     1621 cgtctaacac cgcgcgtgcg ggcggcattg tgttaccgat catcaacagc gtggcggtgg
     1681 ctttggggtc cgaaccggaa aaaagtccgc gtcgtgtcgg acattacctg atgatgtcca
     1741 tttacatggt caccaaaacc accagctata tgttctttac cgcaatggcg gggaacattc
     1801 tggcgctgaa aatgatcaac gacattctgc acctgcaaat tagctggggt ggatgggcgc
     1861 tggccgccgg attgccgggc atcattatgc tgctggtcac cccgctggtg atttacacca
     1921 tgtatccacc agaaattaag aaggtggata acaaaaccat cgctaaagcg ggccttgccg
     1981 aactgggacc gatgaaaatc cgcgaaaaaa tgctgctcgg tgtctttgtg ctggcgctgc
     2041 tgggctggat tttcagtaag tctctggggg ttgatgaatc ttcagtggct attgttgtta
     2101 tggcgaccat gctgctgctg ggtatcgtta cctgggaaga cgtggttaaa aataaaggcg
     2161 gctggaatac cttaatctgg tacggcggta ttatcggctt aagctcctta ttatcgaaag
     2221 ttaaattttt cgaatggtta gctgaagtct ttaaaaataa cctggcattt gatggtcacg
     2281 gtaacgttgc tttcttcgtt attattttcc tcagcatcat cgtgcgttat ttcttcgctt
     2341 ccggtagtgc ctatatcgtt gctatgttac cggtatttgc catgctggcg aacgtctccg
     2401 gcgcaccgtt aatgttaacc gcgctggcac tgttgttctc caactcctat ggcggcatgg
     2461 ttactcacta tggcggcgcg gcaggtccgg tcatctttgg cgtgggttat aacgatatta
     2521 aatcctggtg gttggtcggt gcggtactga cgatattaac cttcctggtg catatcaccc
     2581 tcggcgtgtg gtggtggaat atgctgatcg gctggaacat gctgtaaata tactcgtcat
     2641 acttcaagtt gcatgtgctg cgtctgcgtt cgctcacccc agtcacttac ttatgtaagc
     2701 tcctggggat tcactcgctt gtcgccttcc tgcaactcga attatttaga gtatatccat
     2761 ttattttctt gctgcgcact tcacggtgcg cagatatctg gagcatttga tgatcaagtt
     2821 atctgaaaaa ggcgtgtttc tcgccagtaa taacgaaata attgccgaag aacatttcac
     2881 cggcgaaatt aaaaaagaag aagcaaaaaa aggcactatt gcctggtcta ttctctcttc
     2941 tcataatacg tccggaaata tggataaact taaaattaag tttgattcat tagcctctca
     3001 cgatattacc tttgttggta ttgtacagac cgctaaagcg tccggtatgg aacgtttccc
     3061 gctgccgtat gtgctgacca actgccataa ctcactctgc gccgtcggcg gcaccattaa
     3121 cggtgatgac catgtttttg gtttatcggc agctcagcgt tatggcggta tttttgtgcc
     3181 tccgcatatt gcggtcatcc atcaatatat gcgtgagatg atggcaggtg gcggcaaaat
     3241 gatcctcggg tcagacagtc acacccgtta cggtgcatta gggacaatgg cagtcggtga
     3301 gggcggcggc gagttggtaa aacaattgct taatgacacc tgggatatcg actatccggg
     3361 agttgttgcg gtgcatctga ccggaaaacc agcgccgtat gtggggccgc aggatgtggc
     3421 gttggctatc atcggtgccg tgttcaaaaa cggctacgtc aaaaacaaag tgatggaatt
     3481 cgtaggtccc ggtgttgctg cgctctctac cgatttccgt aacagcgttg acgttatgac
     3541 cactgaaacg acctgtttaa gttctgtctg gcaaaccgat gaagaagtcc ataactggct
     3601 ggcgctgcac ggtcgcggcc aggattactg ccagcttaac cctcaaccga tggcgtacta
     3661 cgatggctgc atcagcgttg atttaagcgc catcaaacca atgattgcgc tgccgttcca
     3721 cccgagcaac gtgtatgaaa tcgacacact gaaccagaac ttgaccgaca ttctgcgtga
     3781 gattgaaatt gagtccgaac gcgtggcgca cggtaaagcc aaactctcgc tgctggacaa
     3841 agtggaaaat ggtcgcctga aagtgcagca ggggattatc gcgggctgtt ctggcggtaa
     3901 ctacgaaaac gtcatcgcgg cggcgaatgc actgcgcggt caatcctgtg gcaatgacac
     3961 cttctcgctg gcggtttacc cgtcatcaca gccggtgttt atggatctcg ccaaaaaagg
     4021 tgtggtagca gatttgattg gcgcaggcgc aatcatcaga accgcgttct gcggcccatg
     4081 ctttggcgcg ggcgatacgc caatcaacaa cggtttgagt attcgccaca ccacgcgtaa
     4141 cttcccgaac cgcgaaggct ctaagccagc taatgggcag atgtcagcgg tggcgttgat
     4201 ggacgctcgt tctatcgctg cgactgcggc aaacggtggc tatttaacct ctgccagcga
     4261 actcgattgc tgggacaacg tgccggagta cgccttcgat gtaacgccgt ataaaaatcg
     4321 cgtctatcaa ggctttgtga aaggggcggc ccagcaaccg ctgatttacg gaccgaacat
     4381 taaagactgg ccggaattgg gtgcgctgac tgacaatatc gtcctcaaag tgtgctcgaa
     4441 gatcctcgac gaagtgacca ccaccgacga attgattcct tccggtgaaa cctcttctta
     4501 tcgttcaaat ccgattggtc tggcggagtt taccctgtca cgccgcgatc caggttatgt
     4561 tggcagcagt aaagcgactg ctgagctgga aaatcagcgt ctggcgggga atgtcagcga
     4621 gctgacagag gtgtttgcgc gcattaagca gattgctggt caggagcata ttgatccgct
     4681 gcaaactgaa attggcagca tggtctatgc ggtgaaacca ggcgatggtt ctgcgcgtga
     4741 acaggcggcg agttgtcagc gtgtgattgg cggtctggcg aatattgccg aggagtacgc
     4801 gactaaacgc tatcgttcta acgtcatcaa ctgggggatg ttaccgctgc agatggtgga
     4861 agcgccaacc tttgaagtgg gggattacat ttacatccct ggcattaaag cggcgctgga
     4921 taatccgggt acgacgttta aaggttatgt gatccatgaa gatgcgccgg taacggaaat
     4981 tacgctctat atggaaagtc tgactgctga agagcgcgag attatcaagg cgggtagttt
     5041 gattaacttc aataaaaacc gtcagatgta aaaagcgcca tgtgaatgta ggtcgcattc
     5101 ggcacttatt gtcggatgcg atgctggcgc atcttatccg acctacaaat cgcatcgaac
     5161 cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata gttaatagct cttacttctt
     5221 cgcctctgca accactttgc tacccacgcc gcggttattg tattcccaca tgcggttgta
     5281 gttagtgtca ttcagattgc gctgtatttc gtcgctatcg tcaacgctgc cggtgttacc
     5341 cgcaaacgga cgattagaga tcaccgcatc gccccacggt ttagccgtgt taaaaccttc
     5401 gttgatggcg ctatcacgga tcaccacctg accgttggta ttggcatcaa catccagcga
     5461 gcggcccagt tgtgccacgc catcaccgga agcattgaaa cggctgttta cggcgaggaa
     5521 accgtagtaa atgttggaca gcgtagccgg tgcaaacaca tacgcttctt gctgagtacg
     5581 tgagttcacc acgcggaatt cggtgttatc gaacaccact gcgccgcgac cagaaacgat
     5641 atccacatcc ccttcaatgt agctgttggt caccagcgta cgcggctgac gattcgtttc
     5701 cagacggttc tgcacaccgc tgttggtgac aaagaaggtg ttctgacgac cgagaatgtt
     5761 aacgttgtta atctgtacct ggtcaccgtc agtacgcagt gccaccgccg gatggttacc
     5821 cgcatctacg ctatcaccca gcgtgttttc gatggtcagg ttttgcagtt gcaggccatt
     5881 gttttgtgac cagaagaccg cagaacacag aacaccgata ctgtcgctgc gtttgctctg
     5941 gcagctatcg tacatatacc acgctggttt acctggcata tatttgccgc gcgggttgac
     6001 gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg gaaagcccaa tcttcacatc
     6061 aatcggtttt tcacctgtac cgtacagagt aattccacct ggagcagcag ggacatacac
     6121 cgttccctga tactcaccag gcatcacggc aatatactgg cgcttgttgg tgcgcttgat
     6181 aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt acaccttgag tacccgccgg
     6241 gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg gacggattcc acgctgcagc
     6301 acctggtgtc agggatgcaa aatagtgttg agcatcgaaa ttctgcgctt cttttgccga
     6361 cagaatcggg cgagaagagg taccaggcgc agtttgatca gaaggacgtt gatcgggtgg
     6421 tgttgagcta caggcggtca gcgtcacgcc aaaagccaat gccagcgcca gacgggaaac
     6481 tgaaaatgtg ttcacaggtt gctccgggct atgaaataga aaaatgaatc cgttgaagtc
     6541 tgctttttta tactaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga
     6601 acaggtcact atcagtcaaa ataaaatcat tatttgattt caattttgtc ccactccctg
     6661 cctctgtcat cacgatactg tgatgccatg gtgtccgact tatgcccgag aagatgttga
     6721 gcaaacttat cgcttatctg cttctcatag agtcttgcag acaaactgcg caactcgtga
     6781 aaggtaggcg gatccccttc gaaggaaaga cctgatgctt ttcgtgcgcg cataaaatac
     6841 cttgatactg tgccggatga aagcggttca cgacgagtag atgcaattat ggtttctccg
     6901 ccaagaatct ctttgcattt atcaagtgtt tccttcattg atatcccgag agcatcaaca
     6961 tgcaatgttg tagggatggc aatttttacg cctgttttgc tttgttcgac ataaagatat
     7021 ccatctacga tatcagacca cttcatttcg cataaatcac caactcgctg cccggtaaca
     7081 acagccagtt ccattgcaag tctgagccaa catggtgatg attctgctgc ttgataaatt
     7141 ttcaggtatt cgtcagccgt aagtcttgat ctccttacct ctgattttgc tgcgcgagtg
     7201 gcagcgaccg ggtttgttgt tatatggcct tcagctattg cctctcggaa tgcatcgctc
     7261 agtgttgatc tgattaactt ggctgacgcc gccttgccct cgtctatgta tccattgagc
     7321 attgccgcaa tttcttttgt ggtgatgtct tcaagtggag catcaggcag ccccctcctt
     7381 attgctttaa ttttgctcat gtaatttatg agtgtcttct gcttgattcc tctgctggcg
     7441 aggatttttt cgtagcgatc aagccatgaa tgtaacgtaa cagaattatc actgttgatt
     7501 ctcgctgtca gaggcttgtg tttgtgtcct gaaaataact caatattggc ctgtattgct
     7561 tcagtgattg ctatcctcct gtctcggcct aaaccaaact ctttacccgt ccttgggtcc
     7621 ctgtagcagt aatatccatt gtttcttata taaaggttag ggggtaaatc ccggcgctca
     7681 tgacttcgcc ttcttcccat ttctgatcct cttcaaaagg ctacctgtta ctggtcgatt
     7741 taagtcaacc tttaccgctg attcgtggaa cagatactct cttccatcct taaccggagg
     7801 agggaatatc ctgcattcgc gcacccatcg acgaactgtt tcaaggcttc ttgggcgtcg
     7861 ctggcgtgcg ttccactcct gaagtgtcaa gtacatcgca aagtctccgc aattacacgc
     7921 aagaaaaagc cgccatcagg cggcttggtg ttctttcagt tcttcaattc gaatattggt
     7981 tatgtctgca tgtgctatct gcgcccatat catccagtgg tcatagcagt cattgatgtt
     8041 ctctgcttcg ataactctgt tgaatggttc tccattccat tcacctgtga ctcggaagtg
     8101 catttatcat ctccataaaa caaaacccgc cgtagcgagt tcagataaaa gaaatcctcg
     8161 tcagtgcgag gatgctgttc attgctgcta tacacttttt tgctctcaac gtaagcggta
     8221 gctcattctg ttgggttggt gcagttgctt ttaggaaatg ctatttaccc cttaaacgtc
     8281 ggctgaaaga gctaaaatcc atgcaaaaaa tttacgcaat tttgtgtatt attgtgcagt
     8341 aagtaatgag ctattttctg cgcaaaaaat ggatggtaaa tttgtccggg gcaggaaaaa
     8401 ttttatgggc gctaaacatg aaaaaagatt cgtatcctta tttgatttgc atgacagttt
     8461 taggcctgat ctttattttc cttttcttct ggtggcgggc agatatttac agggtcacgt
     8521 ttcttaatca gagtatatcc cactattaca ttctgtttag catgggaata gcttttctgt
     8581 tatctctgtt ttgggttaag aaggggatag taaaacaaag cggctggaag agtctgtcag
     8641 cataccttaa ggtttatgca gggatgtgca tatttgctgg attttttctg attatacccc
     8701 ttacaacact aacttatttt ttgcctggag agacatcgtc ttatgttgca ccgtatcggt
     8761 atacttccgg tagttcaaaa agttgttctg gagctgaggt ggatgacccc gatctacatg
     8821 agaatattcg catttgctat ccgtatggca attatgagta cgataatatt atctatgttg
     8881 aaaagaaaat taatacatta ggtgcggtag taacatatgc acagaccgcg cgtgatgata
     8941 ctgaatgaga tagtatatag cgggcaagtt ttagttaatt tatcgaggta atataattta
     9001 cctcgactcg tttattctgg tattaatatt tcgctttacg atcgattttt atctgatgat
     9061 atcatgcggt tttcatatac tgacttactg tcttttctcc gttagcgatt ttctcctgct
     9121 cagcgatgat tttatctttg gcttctagtt aatttcgctc acttcgaacc tctctgttta
     9181 ctgataagtt ccagatcctc ctggcaactt gcacaagtcc gacaaccctg aacggccagg
     9241 cgtcttcgct catctatggg atcgccacac tcacaacaat gagtggcaga tatagcctgg
     9301 tggttcaggc ggcgcatttt tattgctgtg ttgcgctgta attcttcaat ttctgatgct
     9361 gaatcaatga tgtctgccat cttccattaa tccctgaatt gttggttaat acgcttgagg
     9421 gtgaatgcga ataataaaaa aggagcctgt agctccctga tgattttgct tttcatgttc
     9481 accgttcctt aaagacgccg tttaacatgc cgattgccag acttaagtga gtcggtgtga
     9541 atcccatcag cgttaccgtt tcgcggtgct tcttcagtac gctacggcaa atgtcatcga
     9601 cgtttttatc cggaaacggc tgtctggctt ttttgatttc agaattagcc tgacgggcaa
     9661 tactgcgaag ggcgttttct tgctgaggtg tcattgaaca agtcccatgt cggcaagcat
     9721 aagcacacag aatatgaagc ccgctgccag aaaaatgcat tcagtggttg tcataccagg
     9781 tctctctcat ctgcttctgc tttcgccacc atcatttcca gcttttgcga aagggatgtg
     9841 gctaacgtat gaaattcttc gtctgtttct actggtattg gcacaaacct gactccaatt
     9901 tgagcaaggc tatgtgccat ctcaatactc gttcttaact caacaggaga tgctttgtgc
     9961 atatcgcctc ccgtttatta tttatctcct cagccagccg ctgggctttc agcggatttc
    10021 ggataacaga aaggccggga aatacccagc ctcgcttcgt aacggagtag acgaaagtga
    10081 tcgtgcctac gcggatatta tcgtgaggat gcttcatcgc cattgctccc caaatacaaa
    10141 accaatttca gccagtgcct cgtccatttt ttcgatgaac tccggcacca tctcgtcaaa
    10201 acccgccatg tacttttcat cccgctcaac cacgacataa tgcaggcctt cacgcttcat
    10261 acgcgggtca tagttggcaa agtaccaggc atcttttcgc gtcacccaca tgctgtactg
    10321 cacctgggcc atgtaagccg actttatggc ctcgaaacca ccgagcctga acttcatgaa
    10381 atcccgggag gtaaacgggc atttcagctc aaggccgttg ccgtcactgc ataaaccatc
    10441 gggagagcag gcggtgcgca tactttcgtc gcgatagatg atcggggatt cagtaacatt
    10501 cacgccggaa gtgaattcaa acagggttct ggcgtcgttc tcgtactgtt ttccccaggc
    10561 cagcgcctta gcattaactt ccggagccac accggtgcaa acctcagcca gcagggtgtg
    10621 gaagtaggac attttcatgt caggccactt ctttcctgag cggggctttg ctatcacgtt
    10681 gtgaacttct gaagcggtga tgacgccgag ccgtaatttg tgccatgcat catccccctg
    10741 ttcgacagct ctcacgtcga tcccggtacg ctgcaggata atgtccggtg tcatgctgcc
    10801 accttctgct cagtggcttt ctgtttcagg aatccaagag ctttcactgc ttcggcctgt
    10861 gtcagttctg acgatgcgcg aatgtcgcgg cgaaatatct gggaacagag cggcaataag
    10921 tcgtcatccc atgttttatc cagggcgatt agcagagtgt taatctcctg catggtttca
    10981 tcgttaaccg gagtgatgtc gcgttccggc tggcgttctg cagtgtatgc agtattttcg
    11041 acaatgcgct cggcttcatc cttgtcatag ataccagcaa atccgaaggc cagacgggca
    11101 cactgaatca tagctttatg ccgtaacatc cgtttgggat gcgactgcca cggccccgtg
    11161 atttctctgc cttcgcgggt tttgaatggt tcgcggcggc attcatccat ccattcggta
    11221 acgcagatcg gatgattacg gtccttgcgg taaatccggc atgtacagga ttcattgtcc
    11281 tgctcaaagt ccatgccatc aaactgctgg ttttcattga tgatgcggga ccagccatca
    11341 acgcccacca ccggaacgat gccgttctgc ttatcaggga aggcgtaaat ttctttcgtc
    11401 cacggattaa ggccgtactg gttggcgacg atcaacaatg cgatgaactg cgcatcgctg
    11461 gcatgaaagc gcgatgggga gacagggcgg catgagacga cagcgacgaa gtttcaccga
    11521 catcatctgc gaaaactgca aataccttcc aacgaaacgc tccagaaata aacgcaagcc
    11581 aatcccaaaa gaatctgacg taaaaacctt caactacacg gctcacctgt gggatatccg
    11641 gtggctaaga cattgtgcga ggaaaacaag gtgattgacc aaaatcgaag ttacgaacaa
    11701 gaaagcgtcg agcgggcttt aacgtgcgct aactgcggtc agaagctgca tgtgctggaa
    11761 gttcacgtgt gtgagcactg ctgcgcagag ctgatgagcg attcgaatag ctcgatgcac
    11821 gaggaaaaag atgatggcta aaccagcgcg aagacgatgt aaaaacgatg aatgtcggga
    11881 atggtttcac cctgcattcg ctaatcagtg gtggtgctct ccagagtgtg gaaccaagat
    11941 agcactcgaa cgacgaag
//
LOCUS       DABSYT020000124        11899 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.124, whole genome shotgun sequence.
ACCESSION   DABSYT020000124 DABSYT020000000
VERSION     DABSYT020000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.124"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            78..680
                     /gene="lpfA2"
                     /locus_tag="ID725_RS20740"
                     /old_locus_tag="pgaptmp_004148"
     CDS             78..680
                     /gene="lpfA2"
                     /locus_tag="ID725_RS20740"
                     /old_locus_tag="pgaptmp_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HGV7852632.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
     gene            841..1167
                     /locus_tag="ID725_RS20745"
                     /old_locus_tag="pgaptmp_004149"
     CDS             841..1167
                     /locus_tag="ID725_RS20745"
                     /old_locus_tag="pgaptmp_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGV7852633.1"
                     /translation="MATAGIVVGGTRVIFYGDKPDTTISIFNKETNLPYLLQTWVDPF
                     NKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKNIPPSIDKK
                     IPMFLR"
     gene            1320..1469
                     /locus_tag="ID725_RS20750"
                     /old_locus_tag="pgaptmp_004150"
     CDS             1320..1469
                     /locus_tag="ID725_RS20750"
                     /old_locus_tag="pgaptmp_004150"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7852634.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            1491..4025
                     /locus_tag="ID725_RS20755"
                     /old_locus_tag="pgaptmp_004151"
     CDS             1491..4025
                     /locus_tag="ID725_RS20755"
                     /old_locus_tag="pgaptmp_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGV7852635.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            4037..5107
                     /locus_tag="ID725_RS20760"
                     /old_locus_tag="pgaptmp_004152"
     CDS             4037..5107
                     /locus_tag="ID725_RS20760"
                     /old_locus_tag="pgaptmp_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7852636.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            5135..6217
                     /locus_tag="ID725_RS20765"
                     /old_locus_tag="pgaptmp_004153"
     CDS             5135..6217
                     /locus_tag="ID725_RS20765"
                     /old_locus_tag="pgaptmp_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7852637.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            6429..7469
                     /gene="pstS"
                     /locus_tag="ID725_RS20770"
                     /old_locus_tag="pgaptmp_004154"
     CDS             6429..7469
                     /gene="pstS"
                     /locus_tag="ID725_RS20770"
                     /old_locus_tag="pgaptmp_004154"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGV7852638.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            7556..8515
                     /gene="pstC"
                     /locus_tag="ID725_RS20775"
                     /old_locus_tag="pgaptmp_004155"
     CDS             7556..8515
                     /gene="pstC"
                     /locus_tag="ID725_RS20775"
                     /old_locus_tag="pgaptmp_004155"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGV7852639.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            8515..9405
                     /gene="pstA"
                     /locus_tag="ID725_RS20780"
                     /old_locus_tag="pgaptmp_004156"
     CDS             8515..9405
                     /gene="pstA"
                     /locus_tag="ID725_RS20780"
                     /old_locus_tag="pgaptmp_004156"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGV7852640.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            9588..10361
                     /gene="pstB"
                     /locus_tag="ID725_RS20785"
                     /old_locus_tag="pgaptmp_004157"
     CDS             9588..10361
                     /gene="pstB"
                     /locus_tag="ID725_RS20785"
                     /old_locus_tag="pgaptmp_004157"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGV7852641.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            10376..11101
                     /gene="phoU"
                     /locus_tag="ID725_RS20790"
                     /old_locus_tag="pgaptmp_004158"
     CDS             10376..11101
                     /gene="phoU"
                     /locus_tag="ID725_RS20790"
                     /old_locus_tag="pgaptmp_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGV7852642.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            11526..11660
                     /locus_tag="ID725_RS20795"
                     /old_locus_tag="pgaptmp_004159"
     CDS             11526..11660
                     /locus_tag="ID725_RS20795"
                     /old_locus_tag="pgaptmp_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852643.1"
                     /translation="MAISSYSTVTDKFANLTKMPDENNGLQKNQKNFVCLLVMRRSIQ
                     "
     gene            11627..>11899
                     /locus_tag="ID725_RS20800"
                     /old_locus_tag="pgaptmp_004160"
     CDS             11627..>11899
                     /locus_tag="ID725_RS20800"
                     /old_locus_tag="pgaptmp_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HGV7852644.1"
                     /translation="MSVGNEEKYSITHKDITNLIAGDRQSFLKLTLWEFILDLFPMYH
                     IKEAKETIVEFVTKTEDKVKRFERIKSLAIPEEKWRFSTTTEFTVDE"
BASE COUNT         3140 a         2736 c         2932 g         3091 t
ORIGIN      
        1 aaataccgat caggtttatc caaatttaat aaatggataa cacacaggaa taatatcaaa
       61 ttaatggagt tatatttatg aaaccaaata tgattgtagg agcattagcg ttaacttctg
      121 tgtttatggc aggtcaccta caggcggctg atggaacagt ccatttccgt ggtgaaatta
      181 ttgacagtac ttgcgaagtc actcctgaaa ctaaagatca ggtcgttgat ttaggcaaag
      241 taaaccgtac agcctttagt ggcgtcgatg atgtggctgc cccgacggct ttttctatcg
      301 atctgactca atgcccggaa acctttaagt ccgccgcaat tcgtttcgat ggtaatgaag
      361 atgctcatgg taatggcaac ctggcaattg gtaccccgct ggataactct aacgatgctg
      421 ccgctggtat tagcccgagt gataacagtg gggattatac tggtgcgggt gccgttagtg
      481 cagcgaaagg cgtagctatt cgtttatata accgtgcaga taacactcag gtcaagttat
      541 atgaaaattc tgcatcaact ccgatttcta atggtaatgc atccatgaag ttcatggctc
      601 gttatattgc tacggaaacg actattgacc ctggtacagc taacgccgac tcgcagttta
      661 cagttgaata tataaaataa atcattctta acataatacc ttaaataaaa tggcgctcgc
      721 cattatggtt ctcttttact taaatgaagg gcgtcattat tatatttttt atgagataca
      781 tttatgccta tgtatttact tagattgttt tttgctgtta ctttgattac gcagatgtcg
      841 atggctacgg ctggtattgt tgttggggga actcgcgtta ttttttatgg tgacaaacct
      901 gataccacaa tctctatttt taataaagaa acgaaccttc cttatctatt gcagacatgg
      961 gttgatccat ttaataaaga ggataaaagc aaaccgccat tttctgtgat acctcccgtt
     1021 tctcgcattg aaccacagca agagaaaata ttacgcattt taaaaacggc tggcgagtta
     1081 ccaaaagatc gggagtccgt attctggttg aacgtgaaaa acatccctcc ttcaattgat
     1141 aaaaaaatac caatgttctt gagatagcca taaaaacaag aattaagtta ttctggcgac
     1201 cagccgcggt aaagataatc cctgagcagg ctattaaaaa tgttaaatgg tcgcggcagg
     1261 gtaataatct catcgtcgaa aatccaaatc cgatccatat taatgtaatg agtgtgacgg
     1321 tggatgataa agacgtgtcg ctaaatatga tccgcccttt tgaaatattg acattaccaa
     1381 ttccggctgg cgtggcagga aaatcgctcg tttggcgctt tattaatgat tacggtgcaa
     1441 ttagtcaacc actaaagaaa aacctttaag cgccggataa ggatatatcg atggcctctt
     1501 cgcatctttt cattacgctt gcatcgggca tatgtctgct ctgttccata tctgcttttg
     1561 cccgggatag cttgttcaac cccagattac tggaactgga tcatcctgcg gataatattg
     1621 atattcacca gttcaaccgt tcgaatacct tacctgcggg aacatacaaa gttgatgtga
     1681 tgatcaacgg catgctcttc gaacgccagg aagttaaatt cgcccaggat aaccctgatg
     1741 ctgaactcca cccatgctac gtggcgataa aaaacgtgct ggcgacctat ggtataaaag
     1801 ttgatgcgat aaaatctctg gcgaatgttg atgacaaaac atgcgtaaat ccagttccgc
     1861 tgatcgacgg ggctacctgg ttactggacg ccagtaaact tgcattgaat attactattc
     1921 cgcaaattta tctcaacaat gcagttaatg gttatatcag cccttcccgt tgggatcagg
     1981 ggatcaatgc catgatgatg aattatgatt tttcggcatc gcataccatc cggtcaaatt
     2041 atgacgacga cgatgacagt tattatctga atttgcgtaa tggtattaat ttaggcgcat
     2101 ggcgttttcg taattacagc accctgaatt cttatgacgg taatgtggac taccattccg
     2161 tcagtaatta cattcagcgc gacatcatgg cattacgtag ccagattatg attggcgata
     2221 cctggacggc aagcgatgta tttgatagta cacaggtgcg tggcgtgcgg ctgtataccg
     2281 atgacgatat gttgccctcc agccagaacg gctttgcgcc agtggtacat gggattgcga
     2341 aaactaacgc cacggtgatc atcaaacaaa acggctacgt tatttatcaa tcagccgtac
     2401 cacagggcgc atttgccctc accgacttaa acacgaccag tagcggcggc gatctcgatg
     2461 tcactatcaa agaagaagat ggcagcgagc agcactttat tcagccattt acttcactgg
     2521 ccattctcaa gcgtgaaggt cagaccgatg tagaccttag cattggagaa gtgcgcgacg
     2581 aaagcggctt tacgcctgag gtcttgcagt tacaagcaat gcacggtttc cctttgggaa
     2641 taactttgta tggcggaaca caattggcaa atgattacgc ttctgccgcg ctgggtattg
     2701 gtaaagatat gggggcgctg ggcgcgattt cttttgacgt gactcatgcc cgctcgcagt
     2761 ttgactacga cgataatgag agtggtcaat cgtatcgttt tctctattcc aaacgttttg
     2821 aagacaccaa taccaccttt cgtctggtgg gttatcgcta ctctatggag gggttctaca
     2881 ccctcaatga atgggtgtcg cgacaggata atgattctga tttctgggta acgggcaacc
     2941 gtcgcagccg cttcgaaggt acctggacgc aatctttcac gccaggctgg ggcaatattt
     3001 atttaacatt cagtcgacag gaatactggc agaccgatga ggtcgaacgt ttattacagt
     3061 tcggctataa caacaactgg cgaaacatct cctggaacgt ttcctggaac tatacggact
     3121 cgatcaagcg ctcattgggc aaccatcatg atgataacaa tgatgatttc ggcaaagaac
     3181 agattttcat gttctcaatg tcgataccgc tatcgtgctg gatggaagac agctacgtca
     3241 actattcgtt aacgcaaaac aaccaccatg aaagcacgat gcaggtcggt ctgaacggaa
     3301 cgatgctgga agggcgtaac ctgtcttata acgtacagga atcgtggatg cactctcctg
     3361 atgactccta cagcggcaat gccggaatga cctatgacgg gacttatggc tcggtcaatg
     3421 gtagctattc ctggagccgt gactcccaac attttgatta tggcgccaga ggcggcgtgc
     3481 tggtgcatag tgacggagtg accttctcgc aggaactggg cgaaacggtg gcattggtca
     3541 aagcgccggg cgcagaaggc ctgtccattg aaaacgccac cgggatttct accgactggc
     3601 gtggttatac cgtaaaaacg cagcttagcc cgtatgacga aaaccgcgtg gcattgaaca
     3661 gcgactattt ctccaaagcc aatattgaac tggaaaacac cgtcatcaac ctggtaccaa
     3721 cgcgcggtgc ggtggtgaaa gccgaatttg tcacccatgt cggttatcgc gtgctattta
     3781 acgtccgcca ggtcaacggt aaaccaataa tgtttggcgc gatggcaacc gcctctctcg
     3841 aaacgggcac agtcaccggg attgtcggtg ataacggcga actgtatctc tccgggatgc
     3901 ctgaaaaagg cgagttttta ttgagttggg gacaagctgc ggatgaaaaa tgtaaggcgg
     3961 cctatcacat cacccataaa cctgatgata ccagcctggt tcaaatggat gcgatttgtc
     4021 gctaaaggaa gcatgaatga aaaaaatatt atcagggttg attctgctgc tttgctgtcc
     4081 ttatggtttc gccgctaatg gtgatggcgc aacgcacatg tcaaatttat catttggtcc
     4141 gctgacggtg gcagcggcga ataaccactc cggatacaat attttcgagg cactgagcaa
     4201 cacgactgga acatacccgg tgcgctgtca ctgtgatgac acgcatggcg gaccgggcca
     4261 acaaacagca ttttttccta tcttctacac gggagatgcc gcaccggggc ttgtgcttga
     4321 gcgcactctt aatgggttaa attactatgc tctgaatgat tatttatcgg tcggcgtgac
     4381 gatttttatt attaataacc aatatgcggc cattcctttt gaacacttat ccaaccaatc
     4441 cacctcaccg caacatacct gtggagcagg taataatggg agcactgtaa atctggattc
     4501 agggcgctcg gcaaaattat ctttctatgt tcggcattct attactggca cggtgacaat
     4561 acccacaacg gaagtcgcct ggttgtacgc gggcatgtcc gatcattttc ccaaaacgac
     4621 ccccgtttct aaagtgacaa ttcgcggaca actaacggcc ccgcagaact gtgagttaac
     4681 gccaaatcag agcatcgatg tcgattttca aaaaattaat agcgctgagt tctcctcaac
     4741 ggcgggttca attattgcag aaagaaagat taaaaccgaa gtcacagtat cctgtaccgg
     4801 gatggaagac gtaaggtcca cggaggtggt gagtgcgtcg atgattgcgg caaacagaag
     4861 tgccgatgcc accatgatcg tgacgagtaa tccggatgtg ggaattaaga tttttgataa
     4921 gaacgaccgt ccagtgaatg tggatggggg caacttaccc gctgatatgg gtgctattag
     4981 tcgattagga aaaaccgatg gtagcgtaac gttttattca gcgcccgcca gtctgacggg
     5041 cgcaaaacca gcgcctgata atggatttac cgctacagcc acgctggtta ttgaatttac
     5101 taactaatct tgtgttagta aaaggaataa tttcatgaaa aaatggcatt atatattttg
     5161 cataattctc tttcatttag ggttaccgtg cgggtatgcg gcaaatgatg gcacgtgtgc
     5221 aacaagaggc ggcacacata cattaagcct taattttcct ctgacaacgg tcagtgcagc
     5281 aaacaatgtg cctggaaata cattaataga tattgctaat gcaacatctt ctgaaaatta
     5341 tagcgttctg tgtaactgtg attcaaaaca tagcaatggc gcttatcacg aaatatatta
     5401 taccgcagac cctgctcccg gtatggttta tagcaccacc gcaagtggtc ttgcttttta
     5461 ctatcttaac gaatatgtcg atgtgggaac aaaaatatct gtgctaaatg cggggtatac
     5521 ggcagttcct tttgaacatg tttccaacca ggcaactaca acagatcaca cttgtcaggg
     5581 aaacaaaact acagcggttg gcgtgagcct gaaaactgga gcagatgcga agatttcatt
     5641 tcgtattaaa cgttcaataa atggaacggt agtaatacct atcaccgata ttgcattgct
     5701 gtatgccaac atatccagca ccacgacccg tggtgaggcg attgcaaaag ttcgaatttc
     5761 aggcagtttg accgcaccac agtcttgtca gataaatgca ggacaggtga tttattttga
     5821 ttttgatact attcctgcgt ccgaattttc atctaccgcc gggcaagcca ttacttcacg
     5881 aaaaatcact aaaacagtga gtattgagtg tacggggatg gggtatgagc gtacgcagaa
     5941 agtcgatgct tcttttacgg ggacgaaccg aagcagtgac gatacgatgg tggcgacaga
     6001 caatgctgat gtcgggatca aaatttacaa taaatcgaat gctgaagtta gcgtcaacaa
     6061 cggcaagtta cccgcagaca tgggcaacac gaccattttt ggtcgtaaaa atggttcggt
     6121 aactttttcg gcagcacctg ccagctttac cggtgcccgg cctcagcccg gcgtttttaa
     6181 cgctaccgcg accttaacca ttgaatttgt aaactaacct tttgcggctg ttttatgaca
     6241 gccgttcata tttcctctcc tttcatgaaa aaatgctgat atttttcccc ttcaatatca
     6301 ataagtaata actttccttt gggtgtcatc aaactgtcac attccttaca tataactgtc
     6361 acctgtttgt cctattttgc ttctcgtagc caacaaacaa tgctttatga atcctcccag
     6421 gagacattat gaaagttatg cgtaccaccg tcgcaactgt tgtcgccgcg accttatcga
     6481 tgagtgcttt ctctgtgttt gcagaagcaa gcctgacagg tgcaggtgca accttccctg
     6541 cgccggtgta tgccaaatgg gctgacactt accagaaaga aaccggtaat aaagttaatt
     6601 accagggtat cggttcttcc ggtggcgtaa aacagattat cgccaatacc gttgattttg
     6661 gtgcttctga cgcgccgctg tctgacgaaa aactggctca ggaaggtctg ttccagttcc
     6721 cgaccgtgat tggcggcgtg gtgctggcgg ttaacattcc aggtctgaag tctggtgagc
     6781 tggtgctgga tggtaaaacc ctcggcgata tctacctggg caaaatcaag aagtgggatg
     6841 atgaagccat cgccaaattg aatccgggtc tgaaactgcc ttcacagaac attgctgtag
     6901 tgcgccgcgc agatggctcc gggacttcct tcgtcttcac cagctacctg gcgaaagtga
     6961 atgaagagtg gaaaaacaac gttggtactg gctctaccgt aaaatggccg atcggtctgg
     7021 gtggtaaagg taacgacggt atcgctgcgt tcgttcagcg tctgccgggt gcaattggtt
     7081 acgttgaata tgcttatgcg aagcaaaaca acctggcgta caccaaactg atctccgctg
     7141 atggtaaacc tgttttgccg accgaagaaa acttcgctaa tgcagcaaaa ggtgcagact
     7201 ggagcaaaac cttcgctcag gatctgacca accagaaagg cgaagatgcg tggcctatta
     7261 cctctaccac gttcattctg atccacaaag atcagaagaa accagaacaa ggcacagaag
     7321 tgctgaaatt cttcgactgg gcgtacaaaa ccggggctaa acaggcgaac gacctggatt
     7381 acgccagcct gccggatagc gtagttgaac aggttcgcgc tgcgtggaag accaatatta
     7441 aagacagtag cggcaagccg ctgtactaat aaaactccag gccggataag gcattaaacg
     7501 gcttatccgg cattacaaaa tgactttgta aacgcgttta actgaagagt aacttatggc
     7561 tgcaaccaag cctgctttta acccaccggg taaaaagggc gacataattt tcagcgtgct
     7621 ggtaaaactg gcggcgctga ttgtgctatt gatgttgggt ggcattattg tctctctgat
     7681 catctcctca tggccgagca ttcagaaatt tggtctggct ttcctgtgga ccaaagagtg
     7741 ggatgcgccg aacgatatct acggggcgct ggtgccgatc tacggtacgt tggtgacttc
     7801 gtttatcgcg ctgctgatcg ccgtcccggt gagtttcggt atcgccctgt tcctgactga
     7861 gcttgcgcct ggctggctga aacgcccgct gggtatcgcc attgagctgc tggcagccat
     7921 tccaagtatc gtttacggca tgtggggcct gtttatcttt gcgccgctgt tcgccgttta
     7981 cttccaggag ccggtcggca atatcatgtc gaatatcccg attgtcggcg cgctgttctc
     8041 tggcccggca tttggtatcg gtatcctcgc ggcaggcgtg atcctcgcca tcatgattat
     8101 tccgtacatt gcggcggtaa tgcgcgatgt gttcgaacaa accccggtga tgatgaaaga
     8161 gtcggcctac ggtattggct gcaccacctg ggaggttatc tggcgtatcg ttcttccgtt
     8221 caccaaaaat ggtgttatcg gcggcattat gctggggctg ggccgcgcgc ttggtgaaac
     8281 catggcggtg acctttatta tcggtaacac ctaccagctc gacagcgcct cgctgtacat
     8341 gccgggcaac agtatcacct ctgcgctggc gaacgaattt gccgaagcgg aatccggtct
     8401 gcacgttgcc gcactgatgg aactgggcct gatcctgttt gtgattacct tcatcgtcct
     8461 cgccgcatcg aagtttatga ttatgcgcct ggctaagaat gagggggctc gctaatggct
     8521 atggttgaaa tgcaaaccac tgcggcgctg gcggaatccc gccgcaaaat gcaggcgcgt
     8581 cgccgcctca aaaaccgtat tgcactgacg ctctcaatgg cgacgatggc cttcggtctg
     8641 ttctggctga tctggatttt aatgtccacc atcacccgcg gtatcgacgg tatgtcgctg
     8701 gcgctgttca ctgaaatgac gccgccgccc aatacggaag gtggtggtct ggcgaacgct
     8761 ctggcaggta gcgggctgtt aattttgtgg gccacggtat tcggtacgcc gctgggcatt
     8821 atggcgggga tttatctggc tgaatatggt cgtaaatcct ggctggcaga agtgattcgc
     8881 ttcattaacg acattctgct ctctgcgccg tcgattgtgg ttggtctgtt tgtttacacc
     8941 atcgtggtgg cgcagatgga gcacttctcc ggttgggcgg gtgtgattgc gctggcgttg
     9001 ttgcaggtgc cgattgttat ccgcaccacc gagaacatgc tgaaactggt gccggacagc
     9061 ctgcgtgaag cggcttatgc gctgggtaca ccgaagtgga agatgatctc cgcgattacg
     9121 ctgaaagcgt cggtgtccgg gatcatgacc ggtatcctgc tggcgattgc ccgtattgct
     9181 ggtgaaaccg cgccgctgct gtttaccgcg ctctccaacc agttctggag cacggacatg
     9241 atgcagccga tcgccaacct gccggtgacg atctttaagt ttgcgatgag cccgtttgcg
     9301 gaatggcagc aactggcctg ggccggggta ttgatcatta ccctgtgcgt actgctgctg
     9361 aacattctgg cgcgcgttgt tttcgcgaag aataaacacg gttgatattg ctgacacggt
     9421 tttcccctca ccctaaccct ctccccagag gggcgagggg accgaccgag cgcctttttg
     9481 actctgtaca cggttaacac tttgccggat gcggcgtgaa cgcctgatcc ggcctacggt
     9541 aagcctgatt cgcgaagtgc atcaggcacg atgaggaaaa gattgcaatg agtatggttg
     9601 aaactgcccc gagtaaaatt caggttcgta atttgaactt ctactacggc aaattccatg
     9661 ccctgaaaaa catcaacctg gatatcgcca aaaaccaggt aacggcgttt atcgggccgt
     9721 ccggctgcgg taaatcgacg ctgctgcgta ccttcaacaa aatgtttgaa ctgtacccgg
     9781 aacagcgtgc ggaaggtgaa attctgctcg atggcgacaa catcctgacc aactctcagg
     9841 atatcgccct gctgcgtgcg aaagtgggca tggtgttcca gaaaccgacg ccgttcccga
     9901 tgtccatcta cgacaacatc gcctttggcg ttcgtctgtt tgagaagctc tctcgtgccg
     9961 acatggacga gcgcgtgcag tgggcattga ccaaagccgc attgtggaac gaaaccaaag
    10021 ataaattgca tcagagcggt tactctctct ctggtggtca gcaacagcgt ctgtgcattg
    10081 cgcgtggtat cgccattcgc ccggaagtgc tgctgctcga cgaaccatgt tcggcgctcg
    10141 accccatctc caccgggcgt attgaagagc tgatcaccga actgaagcag gattacaccg
    10201 tggtgatcgt cacccacaac atgcagcagg ctgcgcgttg ttccgaccac acggcgttta
    10261 tgtacctggg cgaattgatt gagttcagca acacggacga tctgttcacc aagccagcga
    10321 agaaacaaac agaagactac atcaccggtc gttacggttg attcaggagt gcgttatgga
    10381 cagtctcaat cttaataaac atatttccgg ccagttcaac gccgaactgg aaagtatccg
    10441 cacgcaggtg atgaccatgg gcggcatggt ggagcagcag ctttctgatg caatcaccgc
    10501 gatgcataac caggacagcg atctggcgaa gcgcgtgatc gaaggcgaca agaacgtcaa
    10561 catgatggaa gtggcgatcg atgaagcctg cgtgcgcatt attgccaaac gccagccgac
    10621 ggcgagcgac ctgcgattgg ttatggtgat cagtaaaacc attgccgaac tggagcgtat
    10681 tggcgacgtg gcggacaaaa tctgccgtac tgcgctggag aaattctccc agcagcatca
    10741 gccgttgctg gtaagtttgg agtcgctggg ccgtcatact atccagatgc tgcacgacgt
    10801 gctggacgcg ttcgcgcgga tggacattga cgaagcggta cgtatttatc gtgaagataa
    10861 aaaagtcgat caggagtacg aaggtattgt tcgtcaactg atgacctaca tgatggaaga
    10921 ttcgcgcacc attccgagtg tacttaccgc gctgttctgt gcgcgttcta tcgaacgtat
    10981 tggcgaccgc tgccagaata tttgtgagtt tatcttctac tacgtgaagg gtcaggattt
    11041 ccgtcatgta ggcggtgatg agctggataa actgctggcg ggaaaagacc ccaaagagta
    11101 gtgctaactg taggccagat aaagcttttt gccgcatctg gcaatcagcc cggtcataca
    11161 tagttaccgg gctgatacac tttctcactg aaatattgcc acacaaactc acctcgaaac
    11221 tctccccagt acattgaata tacattattt catttgaatt attttacttt taatgtagtg
    11281 cctcgctata aaaacatatt tgttattctt ttcatttata tcgctacctg cacagagaat
    11341 ggaaaagaaa acaaaaaaca gggtaaaagc cctatatcga gacataaaaa atgagcgatt
    11401 ttttacagta ttcataggtg gtttagtcat aatcgtagca aaccaacacc caggtatgct
    11461 ttttaagaca aatcggcaat gtattatgct cgggcgaata aatattgtgt tcaggagaaa
    11521 tgattatggc tatatcatca tatagtacag ttacagataa atttgctaat ctgactaaaa
    11581 tgccagatga aaataatgga ttgcaaaaaa atcagaaaaa ttttgtttgt ctgttggtaa
    11641 tgaggagaag tattcaataa cccataaaga tataacaaac cttatagctg gtgatagaca
    11701 atcttttctt aaactaactc tttgggaatt tatactcgat ctctttccca tgtatcatat
    11761 taaggaggct aaagaaacaa ttgtagaatt tgtaacgaaa actgaggaca aagttaagag
    11821 atttgagaga attaaatctc tcgctattcc agaagaaaaa tggcgcttta gtacgacgac
    11881 agaatttaca gtagatgag
//
LOCUS       DABSYT020000125        11802 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.125, whole genome shotgun sequence.
ACCESSION   DABSYT020000125 DABSYT020000000
VERSION     DABSYT020000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.125"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..150
                     /locus_tag="ID725_RS20805"
                     /old_locus_tag="pgaptmp_004161"
     CDS             <1..150
                     /locus_tag="ID725_RS20805"
                     /old_locus_tag="pgaptmp_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7852645.1"
                     /translation="DENGIIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWEN
                     IGGMK"
     gene            436..507
                     /locus_tag="ID725_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /pseudo
     CDS             436..507
                     /locus_tag="ID725_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(518..766)
                     /locus_tag="ID725_RS20815"
                     /old_locus_tag="pgaptmp_004163"
     CDS             complement(518..766)
                     /locus_tag="ID725_RS20815"
                     /old_locus_tag="pgaptmp_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGV7852646.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(994..1059)
                     /locus_tag="ID725_RS20820"
                     /old_locus_tag="pgaptmp_004164"
                     /pseudo
     CDS             complement(994..1059)
                     /locus_tag="ID725_RS20820"
                     /old_locus_tag="pgaptmp_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1281..2966
                     /gene="btsS"
                     /locus_tag="ID725_RS20825"
                     /old_locus_tag="pgaptmp_004165"
     CDS             1281..2966
                     /gene="btsS"
                     /locus_tag="ID725_RS20825"
                     /old_locus_tag="pgaptmp_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGV7852647.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            2963..3682
                     /gene="btsR"
                     /locus_tag="ID725_RS20830"
                     /old_locus_tag="pgaptmp_004166"
                     /gene_synonym="yehT"
     CDS             2963..3682
                     /gene="btsR"
                     /locus_tag="ID725_RS20830"
                     /old_locus_tag="pgaptmp_004166"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGV7852648.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            3729..4199
                     /gene="yehS"
                     /locus_tag="ID725_RS20835"
                     /old_locus_tag="pgaptmp_004167"
     CDS             3729..4199
                     /gene="yehS"
                     /locus_tag="ID725_RS20835"
                     /old_locus_tag="pgaptmp_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGV7852649.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(4241..4702)
                     /gene="yehR"
                     /locus_tag="ID725_RS20840"
                     /old_locus_tag="pgaptmp_004168"
     CDS             complement(4241..4702)
                     /gene="yehR"
                     /locus_tag="ID725_RS20840"
                     /old_locus_tag="pgaptmp_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGV7852650.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(4827..6830)
                     /gene="yehQ"
                     /locus_tag="ID725_RS20845"
                     /old_locus_tag="pgaptmp_004169"
     CDS             complement(4827..6830)
                     /gene="yehQ"
                     /locus_tag="ID725_RS20845"
                     /old_locus_tag="pgaptmp_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGV7852651.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            complement(6827..7963)
                     /gene="yehP"
                     /locus_tag="ID725_RS20850"
                     /old_locus_tag="pgaptmp_004170"
     CDS             complement(6827..7963)
                     /gene="yehP"
                     /locus_tag="ID725_RS20850"
                     /old_locus_tag="pgaptmp_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGV7852652.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTPPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(7956..10235)
                     /gene="yehM"
                     /locus_tag="ID725_RS20855"
                     /old_locus_tag="pgaptmp_004171"
                     /pseudo
     CDS             complement(7956..10235)
                     /gene="yehM"
                     /locus_tag="ID725_RS20855"
                     /old_locus_tag="pgaptmp_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(10246..11334)
                     /gene="yehL"
                     /locus_tag="ID725_RS20860"
                     /old_locus_tag="pgaptmp_004172"
     CDS             complement(10246..11334)
                     /gene="yehL"
                     /locus_tag="ID725_RS20860"
                     /old_locus_tag="pgaptmp_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGV7852653.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         2820 a         2987 c         3122 g         2873 t
ORIGIN      
        1 gatgagaatg gcattatccc ggagcaggac agggtaatca cgcaggtggt gatactggat
       61 gcggataaaa agcagataca gtgcgtggta agaccgctgc aaatcctgcg tgctgacggg
      121 acgtgggaaa atattggcgg gatgaagtaa cccgacagct tcacaaaacc ggagtccggc
      181 tccggttttt gtgttgcaat gtccggggga tatttgttag gtagattgta ttgtatgtaa
      241 aggcagagct ccgaatttta ctgttgtaag tgggcctggc gggaatggta tcgccagcat
      301 cattggcttg ggtccagtaa aaagagacgc agaaagatgc acaataggca ctacgcgtca
      361 tgcatggatt aggtcgctca ttatttacac agtactgcta aaaaattatt aaggaaagcg
      421 cggcacttcg tatgtaagag cgtgtcgcgg ttggctggtg aactttcgat agtgcgagta
      481 ttgaatgatt tccagccgtt accgatttac gtgtttatta gcgaacaaac cactcgtcag
      541 cagactccca ggtatctttc agcgtctcct gaacaaaagt tttagctgaa tctttatcgg
      601 cggtgcgcgt aacagaaagg ccatcgttgc tggtggcttt tacgatcacc tctacatcgt
      661 cataacgttt actgatgcgt cgggttaatt cttcctttaa cgcatccaca gcaccgtttg
      721 gcattttagt gattttttct ttagcgatac agatctcaac acgcataaca ccctcctgta
      781 actgtagtta tgtacaggtg ttatttttat ctgtatggat aaccagtgtc aattcctgat
      841 attgtttatg gggcatcaat ggggcatgta tgggacactt tttatcggcg aaattcgtcg
      901 aagttcatcg acatggtaaa cgaatcatct atccaaccct tgaaaaacgg cgctcctgga
      961 cgatcttcgt cgatttttaa aaatgttgcg tcacgcgcgt aacgtgacag ggttaatatc
     1021 acaaagcaac gccacttcac caattgtgta aagcgccatc gtctcaccct tgctcgcgag
     1081 gtcccggttt aactttagac gcagttttgc gaaccaggta gttttgcccg ttttttgtgc
     1141 atttataggg tgattttatt tttgccaggc gattttgagt gatcgtactc acgaattctc
     1201 atttttctgc aagagttcaa agaaagttaa acgcaggcaa tgtatgttac gcgttttaaa
     1261 gggatgtgtg gtttgcgggt atgtacgatt ttaatctggt gttgctgctg cttcagcaga
     1321 tgtgcgtttt tttagtcatt gcgtggttaa tgagtaaaac gccattattc ataccgttaa
     1381 tgcaggtcac ggttcgtctg ccgcataaat ttctctgcta catcgtcttt tccatcttct
     1441 gcatcatggg cacctggttt gggttgcaca ttgacgattc tattgccaat acccgtgcga
     1501 taggcgcggt aatgggcggc ttactcggcg gtccggtcgt cggtgggctg gttggcctga
     1561 ccggcgggtt acatcgatat tcgatggggg gcatgaccgc gctaagttgc atgatctcga
     1621 ccatcgttga aggattgctc ggcggcctgg tacacagcat cctgatccgt cgcgggcgca
     1681 ctgataaagt ctttaacccc attaccgctg gtgccgtcac gttcgtcgct gaaatggtgc
     1741 aaatgctgat catcctggcg atcgcccgac cttatgaaga tgcggtgcgt ctggtgagta
     1801 atattgctgc gccaatgatg gtcaccaata ccgtcggcgc ggcgctgttt atgcgtatat
     1861 tgctcgataa acgcgcgatg tttgaaaaat acacttcggc tttttctgcc actgcgctga
     1921 aagtggcagc ctcgacggaa ggcattttgc gccaggggtt taacgaagtg aacagcatga
     1981 aagtggctca ggtgctgtat ctggagctgg atattggtgc agtcgcgatt accgatcgag
     2041 agaaattgct ggcctttacc ggaattggtg acgaccacca tttacccggc aaaccgattt
     2101 cttcaactta tactttaaaa gcgattgaaa ccggtgaagt ggtctacgct gatggcaacg
     2161 aagtacctta tcgttgctct ttgcatccgc aatgcaaact ggggtcgacg ctggtaattc
     2221 cgttgcgtgg tgaaaatcag cgggtgatgg gcaccatcaa attgtatgaa gccaaaaacc
     2281 gtttatttag ttcaatcaac cgcacgctgg gcgaggggat tgcgcaactg ctttcggcgc
     2341 agatcctcgc cgggcaatat gagcggcaaa aagcgatgct cacccagtca gagatcaaac
     2401 tgcttcacgc ccaggtgaac ccccattttt tgtttaatgc gcttaacacc attaaagcgg
     2461 tgatccgtcg cgacagcgaa caggccagcc agctggtgca gtatctttcc acttttttcc
     2521 gcaaaaactt aaagcggcct tcggagtttg ttactctcgc cgacgaaatt gaacatgtga
     2581 atgcttatct gcaaattgaa aaggcgcgct tccagtcgcg gttgcaggtc aacattgcta
     2641 ttccgcaaga attatcccag cagcaattgc ccgcgtttac cctgcaaccg atagtggaaa
     2701 acgccattaa acatgggaca tcacaactgc tggatacagg gcgagtggca atcagcgtcc
     2761 gacgtgaggg gcaacatttg atgctggaga tcgaagacaa tgccggtttg tatcaaccgg
     2821 taaccaatgc cagtgggctg gggatgaatc tggtggataa gcgtttacgt gaacggtttg
     2881 gcgatgacta tgggataagc gtcgcctgtg agcctgatag ttacacccga ataacgttac
     2941 gactaccatg gagggacgag gcatgattaa agtcttaatt gtcgatgatg aaccgttagc
     3001 acgggagaac ctgcgcgtat ttttgcagga gcagagcgat attgaaatcg ttggtgagtg
     3061 ttcaaacgcc gtggaaggga tcggcgcggt gcataaactg cgcccggatg tgctgtttct
     3121 cgatatccag atgccgcgca tcagtggtct ggaaatggtg gggatgctcg acccggaaca
     3181 tcgtccgtat attgtttttc tcaccgcgtt tgacgaatac gctattaaag cctttgaaga
     3241 acatgccttt gactatctgc tgaagccaat tgatgaagcg cgactggaga aaacgctggc
     3301 gcgattgcgt caggagcgca gcaagcagga tgtttcgctg ttaccggaaa atcaacaggc
     3361 gctgaaattt atcccttgta cggggcatag tcggatttat ttgctgcaaa tgaaagatgt
     3421 ggcatttgtc agcagtcgga tgagcggtgt ctacgttacc agccacgaag ggaaagaggg
     3481 ctttaccgaa ttgacattac gtaccctgga aagtcgtaca ccactactgc gctgccatcg
     3541 tcagtatctg gttaacctcg cgcatttaca ggagattcgt ctggaagata acggtcaggc
     3601 cgagttgatt ttgcgtaatg gcttaaccgt gccggtcagc cgccgttatc tgaaaagctt
     3661 aaaagaggcg attggcctgt aaaagactgc taaaatggct ttttgcctca tcaacacctg
     3721 aaggcctcat gctaagtaac gatattctgc gcagcgtgcg ctacattttg aaagccaata
     3781 ataatgacct ggtgcgtata ctggcgctgg gtaatgtcga agccaccgcg gaacagatcg
     3841 ccgtctggct acgtaaagaa gacgaagagg gttttcagcg ttgtccggac attgttttgt
     3901 cgtcattcct caatggcctg atttatgaaa aacgcggcaa ggatgagtct gctccggcac
     3961 tggagccgga acgtcgcatt aataacaaca tcgtgctgaa aaaattacgc atcgcgtttt
     4021 cgctgaaaac cgatgacatt ctggcgatcc tcaccgaaca gcagttccgc gtttcgatgc
     4081 cggaaattac ggcgatgatg cgtgcaccgg atcataaaaa cttccgcgaa tgcggcgatc
     4141 aatttttacg ttattttctg cgtggactgg cagcgcgcca gcatgtgaag aaaagctaag
     4201 acgggtatgg cggccatgcg caaacatggc cgccgacaga ttatttcact tctttaaaac
     4261 cagcggcttt catcaccagt tccatttgcg ccatagtgat accttttttg gcatcttcag
     4321 cagaaacgtt gattcctgaa ataccctgca gggctttaaa atccactttt tccatatcga
     4381 tagtcacgtt ttcctgcgcg taggtatcgg tataggttaa tttttcttca acacccgcga
     4441 tgtttttgta tttggcgctt aacggctcaa gtgtcttggc agcatcttct ttggtggtag
     4501 caccaatgga ggcaaattga attttggttt cagaagattg cttaagcacc ttgtcacctt
     4561 tgtagacata ggtaatggca atttcagtgc cgttcagatt ggcgctgaat ttcttcgatt
     4621 cttctttgtc accgcagcca gcaagagaaa aaaccagaac agatgcaaca acaagggaaa
     4681 acagcttatt gaaagccttc atgtaaaact ccattttatt taatcaagga actggtgact
     4741 ctcaccaggg gctatatagg atatgcctaa taccgtggcg tgagcagtcc ggaactggag
     4801 tagaactctt agtaaaaagc actatttcat ccttgttgct gaagcagggg gaataattgt
     4861 tcgcacagca aaacaccgtt attcattgct tctacccgtg cctcgctttc tgtattacga
     4921 aattgtccca atacatgtgc cagccgataa aaacccaccg cggtgaggtc attcgccagc
     4981 aactctgcct gaccaatagc actctgttcc tgatagcgcc agccgttatg gagcagttga
     5041 ataagtaatg cctggcagcg catcagcaac tgatgagcag tagacggcac aggcagaacg
     5101 ctggcagaag gtagtgatgc caccacaggc gcagtttctg cgtccagcgc ccaggcacga
     5161 gtctttgtca tcattacctg tggttccagt gtcaattgcc catcaacaaa actgacaaag
     5221 ccagaaacca gacacacggg gtcgtctgtt tgttgcaaaa gcgccgccat gcgttcaacg
     5281 gcataaggcg cgctggctga ggctggcagt gataacgtca gcagattatc ttcaccttcg
     5341 ccgctgatta cctgcgcatc cagcgtctgg cggctgctat cccaaccgag cgaaatacac
     5401 tcagcgaccg gcagaataaa taagttatcg acctgattaa gaggccgtat gcaggagggg
     5461 ggacgctggc gtaaatattc ccgcaaagcc acaatgcccg gctggcgtaa cggcgcgctc
     5521 aacattcgcc aggcatcagg cgacagcggc acaacgctgc ttaagcggtt gcgggtagct
     5581 aacagcagct cgccatcggc actgcgtttt gctgcttgtg aaacaatttg cccacccgcc
     5641 agtgcgccag cctgaaaact aaacagccga cgcgtagctg ccggtgagtt ttcctgttca
     5701 cttcgcggcc aactgcgcga aaggtgcaaa atactgccgg tgtcgggatc ggtaaaccag
     5761 atgcgtaaac cataatgctc aatatcctgc cagcaacgca tacctaaaga caccagccgc
     5821 agatgatcaa gctttgcttc tccggcaatg ccagagccaa cgaccgtgcg ccacggcaca
     5881 ggaggaactt caccaacact gtcgcgccgg gccatctctt gtgcgcaatt taatcgactg
     5941 tttaatgccg caagctgacg taagcattct cctgcatgat agtggctggc gcgggcgtgg
     6001 aaggcatcaa cgctggcgcg cagttgccgt agcgattcac tcacccagcg ccagttgcag
     6061 cgttccgccg cttgctgcgc gcggctgaaa gcggcctcgt agtgaataag cggctggctg
     6121 atgccgccaa gccataatgc ctggcttaat tgctgaacat attgacgaca cgcgttgcct
     6181 tcttcgctgg caaacgggtc gtcagatgat gtgacgtgtt cgctgcgcat ctgccagatt
     6241 aaatgagtaa attccgcttg ctgagttttg gcctcgacga aggcctgcac agccagtacg
     6301 acatgttcgc aaagtgtgcc ttcaatacaa tcacagcggg cgaagcgaat gctgctgcgg
     6361 gaataaaaac gcacatcact catcggtaag cgggctgagg gaatttcgcc aggcgcacag
     6421 aacaactcaa tggtgatgcc tttagcgacc agcacctgtg cacgtttgcg ggtggcatcg
     6481 ggcagggtag ccagttcttc cagccagatt gcaggctgcc attcttcttc tttttccgtg
     6541 ggccgggtgg tggcacaaag tcgctgataa cttaacacca gcatcacgcg atgacggcac
     6601 atgccgctag ccccgcaggt gcactgagcc tctttcagtg cctggccgtt cgccagctgg
     6661 gtacggacac cgtcactgaa ggtggcgatt aaagcgccgt tctcatggct gatttccggg
     6721 acgttgccat tttccagttc cttaaggctg cgcttaacaa aaccggcatt gcttaacgcc
     6781 gtcagggcct gcggcgtgag ttctaataat tccggacgta gtgaattcat gactgcaaat
     6841 tctccgcaag ccaggtagcc agttcacccg gtgtcattgc ggctatttgt gcgccaacat
     6901 taaccagcgc ctgggccata tcgcggtcat agcaaggcgt tgcggtgctg tcgagcgctg
     6961 ccagccccag cactttgatg ccgctctgga cacacttttt cacctgatgc gtcagcaatg
     7021 atgatgaacc cccttcataa aaatcactca cgaggataat gacgcttttt gctggttgtt
     7081 caataagttg ccgcccatac tccacggcac tggcgatatt ggtcccgccg cccaactgta
     7141 ctttcattaa taactctacc ggatcggcaa cgtctgccgt gagatcaacg acactggtgt
     7201 caaacgccac cagatgggta cgaatgccgg gtaactgcca caaacaggcc gccattaccg
     7261 cagagtggat caccgaatcg accatcgatc cgcttagatc aaccagtaag accagttgcc
     7321 attgttcact gtggcgctta attcggctgt taaagcgggg ggattcgata tacaacttgc
     7381 cgtgttgcgg gtgccagtgt tgcagattgg cgcgaagagt acttttgaaa tcaaagtctc
     7441 gcgccagtgg aatagatgag cggcgacggc gatcgcggac accagaaaaa gcctgacgaa
     7501 cttcctttgc cagtcgagcc ataatttctt caacaacctg gcgcactatc cggcgggcgg
     7561 cagccagtac ttcggggttc atcagatgtt tggtgtgcaa aacggcgcgt agcaggcttt
     7621 cagaaggctg catacgttcc agcacgtcga gattcgtcac cacatcttca atgccgtagc
     7681 gcagtacggc atcgctttcc agccgctcaa tcacctgttg cggaaacagc gtgtgaatac
     7741 tgttgatcca ctcagggggg gtgagatttg agccacctaa tccaccggag cgttcaccac
     7801 gctggagccg ttcaggatcg cgcccataca gccactccag cgcgtggtct atctgccggg
     7861 cgttgtcatc cagcccacaa agcgtcgttt ctgccgcttc gccaagaatt aatcgccagc
     7921 gttgtagctc acgggtggtc agaagatcgt tcagttcaga catggaaaac tccccagttt
     7981 tgcagcgatg ccagtgcctg ctgttcgagt tgttgatgat gtgcaatggc ttgtggtggg
     8041 caatgcaacg gcatttgcag cgccgaaacg ggaagttgcg ccagttgata atgttcaagc
     8101 acctgatgcg ccagtgtccc gcgttctcgt ggcggtagcc aggccatcgc cgcgcgtaaa
     8161 tcgggcaggg cattgataaa atcggcatca cttggttgat ttaaatgact gctgaaaccg
     8221 gcgataaatg ccggctgaca ggccagttga tggcgggcca gcgccagcaa accgtgcagc
     8281 gcttcgccgg attgtgccgg ggataactgc gccagcatcg tcagcgccgc ttcggcacta
     8341 gcgttgggat gttccagacg catcagtgcg ccaagagccg cgccgcgatc cagcgccggg
     8401 gcatggattg cctgactgcg tcgctccagt aaggctaccg ccgcagaaag agaaacgccg
     8461 ggtaaattaa cgccgctatg tagatcgcgc agaatatggc aaagcgcttg ccagctatgc
     8521 aggtgagcgt gaaactcctt ttcatccggt ctgccgttag attcacacag ccacagcgtg
     8581 cgatcgataa ccgcgcataa cgtcgtttgt gatatctgcg caccttgcat accgctaatt
     8641 tcatccagcc gccataaggc atatagcact tccagcgccg ggcccatttc ggcaaattga
     8701 ttttcctagg cgattaataa tgtgagttgc tccagtaatt gttgactgaa ggacgccaga
     8761 cccgctaacg ccgcctggct aagacatgtg gtgatactgc cgattccacc cgcgtccagc
     8821 atatcggctt ctaatttatg gcgtgcggct tcctggagtg tggcaccaaa gcaggcggcc
     8881 tcaatcaatg ccgcatgctg gctaagcggg cgggttaatt tccagcgttc ttcaccgttg
     8941 cctgcaagtg tcagtgtgct tccctgctgg cgtacaatcc ctgggatctc cagtattgcc
     9001 agccgatgta acacctgact ttgcgctagt ccattggggt taaagcgatt taaggtaagc
     9061 tccgccgaaa gagagattgc ggtacgttcc agttcgcagg tgacatcttt tggcagaggc
     9121 ggttgtggcg tagaaggggc aagtttaccg aatccgtcac ccgctaatgt gtctatcaac
     9181 gttagcagaa tcggatcggt atcgggatga ataacgccgc gatagctcca cggcaacggc
     9241 gcgttcaggg cttctttaat cagcgagcct gctatggcat ccagccagtc agtgcgtaac
     9301 ggtagtttat atccgcgcaa ctgtgccagc gccatcgcat gcagatgagc ggcagccata
     9361 tccgcggtcg aagcaggcaa gtggtgttgg cgcaaacggg taagaaccgt ttttagcagt
     9421 tgttctcccg cctgctgtaa gccccactgc cagcaccagc tttgccagac cggggcaggc
     9481 attcctgaaa ggtatcctgc cagcacatca aggcgctttt cactgtaggg tgtgagataa
     9541 caacctgtaa cggcatctgc cagcgagggc aattctggct tgttaatgtc ctgcgggcat
     9601 tcgcgccaca tttttgccag tgccggagcg tgccagccac cgcagacgac taacacgtcg
     9661 ccattatttt gctgcactgc ccatgcaatc cagcgagcca taaaggcttc gcgctgacga
     9721 ttgagtgcat cgccggggaa atcgccccgc agttgggcaa aatagtgcgc cagtgcagag
     9781 ggtaatgcag tttgctggct ttcatcttcg aacaagtgat cccacagggt atcgctgtta
     9841 tccatgcggg tggcacgcag cagcaaggcc tggctttcct cttgcgtatc aggcgaatcg
     9901 tcctcttctt cactctgcgc ccagcaaggc aaatcgataa agtaagtttg tgcctgaata
     9961 tgacgtgcgg cttgtagcgc ctgccactcc ggcgaaaatt cggcaaatgg cgtccaggcg
    10021 ccgcgcccag gagcagcacc gtcctgatac tggcaataac tgtaaatagc caccggaagc
    10081 tggtgggcta aaaacagttc atctacccgg tcattaaaat cagccgggcc ttcaatcaac
    10141 acgtatcgtg gtcgctggct ttcgattaac gatttcacca gtcgggcgca ggccggacta
    10201 tgatgccgga tgccgacaat taacggctcg ctcatgcatc tttcctcacg gcaggcggtg
    10261 gcgggcttga taataagcct gccagtgagc ttctttatgt gtggcgacac gctgttcgaa
    10321 gtaacggcgc agacgagcgc gatcttcttc gttatctttg acgatggttc cggcaataca
    10381 gtccaccagg tcggcaggct cccccgcgcg attcgctaaa aaccaggcgc ggacgcccac
    10441 agcgtgggcc acgttcacgg cttcagcggt ggacataatc gctgttagtg tatccattga
    10501 cgttttcttt tcgccgttgg cacgcagatc gcggaaggtg cggaccagta actccagtac
    10561 cgcgtcaggc actttatgcg gaataccgct atgggccagc aaacgcgccg aagcactggc
    10621 gacaagctcc agctcctggg cgaaatccat aatcggaaag acagtttcaa aatcgaagcg
    10681 acgctttagc gcagcactca tttcattgac gccgcgatcg cgggtattgg cagtggcaat
    10741 aatattaaag ccttctcgcg catagagctg actggcttca ccagtgagtt ccggcaccgt
    10801 catcaccctg tcagaaagca tacctaacag acagtcttgc acttccagcg gtgtacgcgt
    10861 tatctcttca aaacggacga ttttgccgtc gcgcattccc tggtaaagcg gggcagggac
    10921 cagcgcttcc gttgatggac catgattgat aagcagggcg tagttccagc cgtacttgat
    10981 ttgatcttcg gtagtagatg cgccgccctg gatggttaac ccggcatcgc cgctaataga
    11041 ggttgccagt aattcagaca ggagagattt tgcggtgcca ggttccccca ccaacatcaa
    11101 accgcgtccg gtggcaagag tgactaacat acgttcgaca gcggagggac taatcaccac
    11161 tttacggcta atattttgcg cgctatcgcc aaggataaaa gcacgggccg caggcaaact
    11221 taactgccaa ccgggcgggc aaggtgcgtt atcattttgt tttaattttg ccagttcatc
    11281 ggcgtataac acagcagcag gcggacgctg cagatgatta ttctgcggtg acatatattc
    11341 atccctgaaa tgtattaatg aaataaacga tattttacgc gcaataaatg tttggcgcat
    11401 tagttgtttg agcgaaaaat atctgggaag cagatcacag taaataaaaa aacctgctgg
    11461 tgaggcaggt tcttttattt actattttca ggcctaacac cacagcctgt gcaacatcgt
    11521 gttccaacga acgaattata accacggctg gaccccgttg caattaagat aatgagattt
    11581 ataatcctga ctgttgggac gttgcgccgg atcgaaccta tttcaagtcc tggctaccgg
    11641 acggggggtg ctgcaggtgg tgccctgatc cggagtgcaa catcgtggtc caacatacgt
    11701 aaaccagtat cgacaggcag tgtaagggta aggtgctggt gttgcgctcc ttaataagga
    11761 gagcggatgg actcgctgac acaaaagtta accgtgctca tt
//
LOCUS       DABSYT020000126        11695 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.126, whole genome shotgun sequence.
ACCESSION   DABSYT020000126 DABSYT020000000
VERSION     DABSYT020000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.126"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            2..166
                     /gene="kil"
                     /locus_tag="ID725_RS20865"
                     /old_locus_tag="pgaptmp_004173"
     CDS             2..166
                     /gene="kil"
                     /locus_tag="ID725_RS20865"
                     /old_locus_tag="pgaptmp_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGV7852654.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
     gene            221..517
                     /locus_tag="ID725_RS20870"
                     /old_locus_tag="pgaptmp_004174"
     CDS             221..517
                     /locus_tag="ID725_RS20870"
                     /old_locus_tag="pgaptmp_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGV7852655.1"
                     /translation="MNAYYIQDRLEAQSWERHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            523..1308
                     /gene="bet"
                     /locus_tag="ID725_RS20875"
                     /old_locus_tag="pgaptmp_004175"
     CDS             523..1308
                     /gene="bet"
                     /locus_tag="ID725_RS20875"
                     /old_locus_tag="pgaptmp_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGV7852656.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKASEQKVAA"
     gene            1305..1985
                     /locus_tag="ID725_RS20880"
                     /old_locus_tag="pgaptmp_004176"
     CDS             1305..1985
                     /locus_tag="ID725_RS20880"
                     /old_locus_tag="pgaptmp_004176"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGV7852657.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARVLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYLAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1982..2164
                     /locus_tag="ID725_RS20885"
                     /old_locus_tag="pgaptmp_004177"
     CDS             1982..2164
                     /locus_tag="ID725_RS20885"
                     /old_locus_tag="pgaptmp_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGV7852658.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            2137..2328
                     /locus_tag="ID725_RS20890"
                     /old_locus_tag="pgaptmp_004178"
     CDS             2137..2328
                     /locus_tag="ID725_RS20890"
                     /old_locus_tag="pgaptmp_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGV7852659.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            2405..2620
                     /locus_tag="ID725_RS20895"
                     /old_locus_tag="pgaptmp_004179"
     CDS             2405..2620
                     /locus_tag="ID725_RS20895"
                     /old_locus_tag="pgaptmp_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGV7852660.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2719..2940
                     /locus_tag="ID725_RS20900"
                     /old_locus_tag="pgaptmp_004180"
     CDS             2719..2940
                     /locus_tag="ID725_RS20900"
                     /old_locus_tag="pgaptmp_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGV7852661.1"
                     /translation="MADLIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            2937..3884
                     /locus_tag="ID725_RS20905"
                     /old_locus_tag="pgaptmp_004181"
     CDS             2937..3884
                     /locus_tag="ID725_RS20905"
                     /old_locus_tag="pgaptmp_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGV7852662.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAAKSKLNEQREYYEGVISDGSKHIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIVVDADYDYD
                     RIVWVDEDGNEANKLQSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            3886..4332
                     /locus_tag="ID725_RS20910"
                     /old_locus_tag="pgaptmp_004182"
                     /pseudo
     CDS             3886..4332
                     /locus_tag="ID725_RS20910"
                     /old_locus_tag="pgaptmp_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4352..4567
                     /locus_tag="ID725_RS20915"
                     /old_locus_tag="pgaptmp_004183"
     CDS             4352..4567
                     /locus_tag="ID725_RS20915"
                     /old_locus_tag="pgaptmp_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852663.1"
                     /translation="MAIAASYTMHLYCDCRQCTNGKYQTPDFGEYIGTSWAGCAKEAR
                     KDGWRINKDKTRAFAPGHKILRSNKGE"
     gene            4569..4787
                     /locus_tag="ID725_RS20920"
                     /old_locus_tag="pgaptmp_004184"
     CDS             4569..4787
                     /locus_tag="ID725_RS20920"
                     /old_locus_tag="pgaptmp_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGV7852664.1"
                     /translation="MPTLFRKEYPRKSRATEFLFLILFIVLMTPISPLIFVWAIGKII
                     ELVIELYNDVVWASFNTLHNKINPYKEN"
     gene            4789..5076
                     /locus_tag="ID725_RS20925"
                     /old_locus_tag="pgaptmp_004185"
     CDS             4789..5076
                     /locus_tag="ID725_RS20925"
                     /old_locus_tag="pgaptmp_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852665.1"
                     /translation="MATLQELIDLTPEQEKAWNRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTASVYMPSIDAPGLTSWADDWHGITLKDGVEVDKD"
     gene            5080..5211
                     /locus_tag="ID725_RS20930"
                     /old_locus_tag="pgaptmp_004186"
                     /pseudo
     CDS             5080..5211
                     /locus_tag="ID725_RS20930"
                     /old_locus_tag="pgaptmp_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5224..5697
                     /locus_tag="ID725_RS20935"
                     /old_locus_tag="pgaptmp_004187"
     CDS             5224..5697
                     /locus_tag="ID725_RS20935"
                     /old_locus_tag="pgaptmp_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGV7852666.1"
                     /translation="MGIELVKYGCNTFICPDNSMEPGNVPLYIGLPRIDPASQTAKLS
                     FQEWLSEQKEKIDVDCGCVSIETLTHWMKSAYEAGNSPVTPDSWISCSDRMPEKGQNV
                     LISVNFDSSLVEPLICSARYTGSTFRRGDATIKPGNGIEQATHWMPLPEPPQEVN"
     gene            5697..5981
                     /locus_tag="ID725_RS20940"
                     /old_locus_tag="pgaptmp_004188"
     CDS             5697..5981
                     /locus_tag="ID725_RS20940"
                     /old_locus_tag="pgaptmp_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGV7852667.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYLAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGSIIVVTTQSDMTIQIHKPKEEGRG"
     gene            6337..6960
                     /locus_tag="ID725_RS20945"
                     /old_locus_tag="pgaptmp_004189"
     CDS             6337..6960
                     /locus_tag="ID725_RS20945"
                     /old_locus_tag="pgaptmp_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGV7852668.1"
                     /translation="MTSQLIPVFNGTIDNETILLVNARDLHSFLGVGRMFAHWVKERI
                     AEYGFVESQDYILICQNGQTKGRGGDRRSKDYHLTLDTAKELAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMEKAQDISKRLYPILKELNSSYASKLYD
                     IAFETFYTVTKNRDALLREATRIDQTSAIFERARPMLKSLRARQFEF"
     gene            7003..7170
                     /locus_tag="ID725_RS20950"
                     /old_locus_tag="pgaptmp_004190"
     CDS             7003..7170
                     /locus_tag="ID725_RS20950"
                     /old_locus_tag="pgaptmp_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852669.1"
                     /translation="MKFKFTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHAEVTNI
                     RIEELKEHQAA"
     gene            7197..7400
                     /locus_tag="ID725_RS20955"
                     /old_locus_tag="pgaptmp_004191"
     CDS             7197..7400
                     /locus_tag="ID725_RS20955"
                     /old_locus_tag="pgaptmp_004191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852670.1"
                     /translation="MSDLAMKVLKWQSTDYVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVALYIDEFENAL"
     gene            7397..8023
                     /locus_tag="ID725_RS20960"
                     /old_locus_tag="pgaptmp_004192"
     CDS             7397..8023
                     /locus_tag="ID725_RS20960"
                     /old_locus_tag="pgaptmp_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HGV7852671.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            7983..8195
                     /locus_tag="ID725_RS20965"
                     /old_locus_tag="pgaptmp_004193"
     CDS             7983..8195
                     /locus_tag="ID725_RS20965"
                     /old_locus_tag="pgaptmp_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGV7852672.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADTELH
                     LFGEILSRKLDELEKLVEEADTSPIA"
     gene            8231..8617
                     /locus_tag="ID725_RS20970"
                     /old_locus_tag="pgaptmp_004194"
     CDS             8231..8617
                     /locus_tag="ID725_RS20970"
                     /old_locus_tag="pgaptmp_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGV7852673.1"
                     /translation="MNAVLTELNKLGKATADSISKGLNIDLNDVIDTLWKLKNQGIVK
                     VKNGIWQAVEGVGSKTDIDSVQPVQPVQPVQPVQPVQNNIIGDLLRKSRKEARRAGRK
                     QKRWEGACKALQELNKYRDLINELSE"
     gene            8696..8878
                     /locus_tag="ID725_RS20975"
                     /old_locus_tag="pgaptmp_004195"
     CDS             8696..8878
                     /locus_tag="ID725_RS20975"
                     /old_locus_tag="pgaptmp_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7852674.1"
                     /translation="MEVNLMTEKEVSDLLQKRRTALYNLRKKHGFPEPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(8862..10031)
                     /locus_tag="ID725_RS20980"
                     /old_locus_tag="pgaptmp_004196"
     CDS             complement(8862..10031)
                     /locus_tag="ID725_RS20980"
                     /old_locus_tag="pgaptmp_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7852675.1"
                     /translation="MALSDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVTDLKPNTQTLYRSVVYNTMYTQFEGSPVASIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVVTSNKVLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPVFTQ
                     VKPYIERLLNAGFDVLFPGQEIDKPIDRSSANLYMKKLREKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            10227..10301
                     /locus_tag="ID725_RS20985"
                     /old_locus_tag="pgaptmp_004197"
     tRNA            10227..10301
                     /locus_tag="ID725_RS20985"
                     /old_locus_tag="pgaptmp_004197"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10260..10262,aa:Arg,seq:cct)
     gene            10463..11620
                     /gene="intS"
                     /locus_tag="ID725_RS20990"
                     /old_locus_tag="pgaptmp_004198"
     CDS             10463..11620
                     /gene="intS"
                     /locus_tag="ID725_RS20990"
                     /old_locus_tag="pgaptmp_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HGV7852676.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
BASE COUNT         3447 a         2626 c         2849 g         2773 t
ORIGIN      
        1 aatggatcaa acacttatgg ctatccagac taaattcact atcgccactt ttattggcga
       61 tgaaaagatg tttcgtgaag ccgtcgacgc ttataaaaaa tggatattaa tacagaaact
      121 gagatcaagc aaaagcattc actacccccc tttcctgttt tcctaatcag cctggcattt
      181 cgcgggcgat attttcacag ccattttcag gagttcagcc atgaacgctt attacattca
      241 ggatcgtctt gaggctcaga gctgggagcg tcactaccag cagatcgccc gtgaagagaa
      301 agaggcagaa ctggcagacg acatggaaaa aggcctgccc cagcacctgt ttgaatcgct
      361 atgcatcgat catttgcaac gccacggggc cagcaaaaaa gccattaccc gtgcgtttga
      421 tgacgatgtt gagtttcagg agcgcatggc agaacacatc cggtacatgg ttgaaaccat
      481 tgctcaccac caggttgata ttgattcaga ggtataaaac gaatgagtac agcactcgca
      541 acgctggcag ggaagctggc tgaacgtgtc ggcatggatt ctgtcgaccc acaggaactg
      601 atcaccactc ttcgccagac ggcatttaaa ggtgatgcca gcgatgcgca gttcatcgca
      661 ttgctgatcg tcgccaacca gtacggtctt aatccgtgga cgaaagaaat ttacgccttt
      721 cctgataagc agaacggcat cgttccggtg gtgggcgttg atggctggtc ccgtatcatc
      781 aatgaaaacc agcagtttga tggcatggac tttgagcagg acaatgaatc ctgcacatgc
      841 cggatttacc gcaaagaccg caatcatccg atctgcgtta ccgagtggat ggatgaatgc
      901 cgccgcgaac cattcaaaac ccgcgaaggc agagaaatca ccggaccgtg gcagtcgcat
      961 cccaaacgga tgttacggca taaagccatg attcagtgtg cccgtctggc cttcggattt
     1021 gctggtatct atgacaagga tgaagccgag cgcattgtcg aaaataccgc atacactgca
     1081 gaacgtcagc cggaacgcga catcactccg gttaacgatg aaaccatgca ggagattaac
     1141 actctgctga ttgccctgga caaaacatgg gatgacgact tattgccgct ctgttcccag
     1201 atatttcgcc gcgacattcg cgcatcgtca gaactgacac aggccgaagc agtgaaagct
     1261 cttggattcc tgaaacagaa agcctctgaa cagaaggtgg ctgcatgaca ccggacatta
     1321 tcctgcagcg taccgggatc gacgtgagag ctgtcgaaca gggggatgat gcgtggcaca
     1381 aattacggct cggcgtcatt acagcttcag aagttcacaa cgtgatagca aaaccccgtt
     1441 caggaaagaa atggcctgac atgaaaatgt cctacttcca caccctgctt gctgaggttt
     1501 gcaccggtgt ggctccggaa gttaatgcca aagcactggc ctggggaaaa caatacgaga
     1561 acgacgccag agtcctgttt gagttcacct ccggcgtgaa tgttactgaa tccccgatta
     1621 tctatcgcga cgaaagtatg cgtacagcct gctctcccga tggtttatgc agtgacggca
     1681 acggccttga actgaaatgc ccgtttacct cccgggattt catgaaattc cggctcggtg
     1741 gtttcgaggc cataaaatcg gcttacctgg cccaggtgca gtacagcatg tgggtgacgc
     1801 gaaaagatgc ctggtacttt gccaactatg acccgcgtat gaagcgtgaa ggactgcatt
     1861 atgtcgtggt tgagcgggat gaaaagtaca tggcgagttt tgacgagatg gtgccggagt
     1921 tcatcgaaaa aatggacgag gcactggctg aaattggttt tgtatttggg gagcaatggc
     1981 gatgaagcat cctcacgata atatccgcgt aggcacgatc actttcgtct actccgttac
     2041 aaagcgaggc tgggtatttc ccggcctttc tgttatccaa aatccactga aagcccagcg
     2101 gctggctgag gagataaata ataaacgagg ggctgtatgc acaaagcatc tcctgttgag
     2161 ttaagaacga gcattgagat ggcacatagc cttgctcaaa ttggagtcag gtttgtgcca
     2221 ataccagtag aaacagacga agaatttcat acgttagccg catccctttc acaaaagctg
     2281 gaaatgatgg tggcgaaagc agaagcagat gagagagacc aggtatgaca accactgaat
     2341 gcattcttct ggcagcgggc tttatattct gtgtgcttat gcttgccgac atgggacttg
     2401 ttcaatgaca cctcagcagg aaaacgccct tcgcagcatt gcccgtcagg ctaattctga
     2461 aatcaaaaaa gccagacagc agtttccgga taaaaacgtc gatgacattt gccgtagcgt
     2521 actgaagaag caccgcgaaa cggtaacgct gatgggattc acaccgactc acttaagtct
     2581 ggcaatcggt atgttaaacg gcgtctttaa ggaacggtga acatgaaaag caaaatcatc
     2641 agggagctac aggctccttt tttattattc gcattcaccc tcaagcgtat taaccaacaa
     2701 ttcagggatt aatgaaagat ggcagacctc attgattcag catcagaaat tgaagaatta
     2761 cagcgcaaca ccgcaataaa aatgcgccgc ctgaaccacc aggctatatc tgccactcat
     2821 tgttgtgagt gtggcgatcc catagatgag cgaagacgcc tggccgttca gggttgtcgg
     2881 acttgtgcaa gttgccagga ggatctggag cttatcagta aacagagagg ttcgaagtga
     2941 gcgaaattaa ttatcaggca ctgcgtgaaa aggcagagaa agcaactaaa ggaagctaca
     3001 tcgtagggca tacatctgtt aaccaacacg gcaatttaac aggagttttt gtttgccaaa
     3061 aatggaaagg agaacccggt ggcgtgattg cagaatgtca tgttaactgc ctggttgaaa
     3121 cagatgctca ggcttacgca aacgctgaat ttattgctgc ctttaatcca aatgttgcgc
     3181 tggcactgct ggatgaacgg gaaagaaacc agcaatacat caaacgccgc gaccaggaga
     3241 acgaggatat tgcgctaacg gtagggaagc tgcgcgttga gcttgaagca gcaaaatcaa
     3301 aactcaacga gcagcgtgag tattacgagg gcgttatctc ggatggaagt aagcacatag
     3361 cagaactgga aaaacaatgc gccgaatggg agcgaaaagc attaagcaac tttgaagagt
     3421 gtgctgcgat ggctgaacgt atcgaagaga tgcagacaaa atctgcacca gattcgtttg
     3481 gcatcatcgg tgaaaatatt cgaacacagg ataatcgaat aacgtcagat cctatgtttt
     3541 gtgtgtatca aaagcgcgaa atcgttgttg atgctgatta tgactatgac cggattgtct
     3601 gggttgatga agatggcaat gaagccaata aactccaaag tcgtcgtctc gaactacttc
     3661 atgaaaactt tcgggagcca ccagaaaaat ggcggcgcgt tgctgtgaaa gatattgatg
     3721 aattcgttac ctgctgtttc accgaacagg gttgtaaaga ttacctagca gtcaatggtc
     3781 acaatcttcg attgccattt atatatgtaa aaagcggttt caggaacgct gaatatatcg
     3841 gcataagaaa ctggcttgct ggcattcgca tcaaaggaga gtgatatgac cactataacc
     3901 gataagaaac agtatcccag cgagcaattt cttaatgagc tgatcaccaa catggagttt
     3961 gctgcaaggg caccagttga agtcgtgaga gcgatggcag cagagctaca gaagcggcgc
     4021 gaagctgata gtgcagaacc agttgcttat attttcaaac atccggccgg gaaattattc
     4081 tgggctttaa cggatgaaag caataaagag caagcggacg ttattcctgt ttatgctgcc
     4141 gcgcctgcgt cggttgtgcc ggataatgca tcagagcctc ttgcttatgc ttacaaagag
     4201 cttacgcctg agattatgcg caaccattta gctgtattcg agcgatatgg aatagcccca
     4261 aacgatagca ctaccacaat tcaggcactg cgaatcgcgc tggatggtat agagcggagc
     4321 gacgccatgc ttcagggtaa aggaggggga tatggctatt gccgcaagtt acaccatgca
     4381 tctctactgt gattgtcgcc agtgtacaaa tggtaaatat caaacgccag atttcggtga
     4441 gtatatcggt acgtcatggg ctggctgtgc aaaagaggcg cgcaaggatg gctggcgaat
     4501 aaacaaagac aaaacgcgtg cttttgcgcc agggcataaa attttgagga gcaacaaagg
     4561 agagtgatgt gcctacatta ttcagaaaag aatatccgcg aaaaagtaga gcgacagaat
     4621 ttttgtttct cattctgttt atcgtgttga tgacaccgat atccccgcta atttttgtct
     4681 gggcaatcgg gaaaataatt gagctagtta ttgagttgta taacgacgtg gtatgggcgt
     4741 cgttcaacac actgcacaat aaaattaacc catataagga aaactgaaat ggcaactttg
     4801 caggaattaa tcgacctgac gccagaacag gaaaaagcgt ggaatcgtct tgtgaaggct
     4861 gtaaaggatt tcagggcagc cggaggaaag ttttatagtg tcctagacac gctgagcgca
     4921 tacaacggcg agcacgttgc cagcattgat aacgataagg gctaccacac tgcaagcgtc
     4981 tatatgccta gcattgatgc gcctgggtta accagttggg ctgatgattg gcacggcatc
     5041 acgctgaaag atggcgttga agtggataag gactaacaca tgacaacgtt caccgacaaa
     5101 gaaatgatta aagaaatcaa agagcgaatc ggcagcctgg acgtgcgaga taatattgag
     5161 cgccgtgctt atgaaattgc gttgactgca ttgaccactg aaccattcgc cactatcgac
     5221 acagtgggaa ttgagctcgt taaatatggt tgtaacacgt ttatttgtcc cgacaattcg
     5281 atggagccgg gaaatgtacc actatatatc ggcctgccac gaattgatcc agcaagccag
     5341 actgccaagc tgtcattcca ggaatggttg tcagaacaaa aagaaaaaat agacgttgat
     5401 tgcggatgtg taagcatcga aacgcttacg cactggatga aatccgctta tgaggctggt
     5461 aactctccgg taactccgga tagttggata agctgtagtg atcgaatgcc tgaaaagggc
     5521 cagaacgtgc ttatttcggt gaatttcgat agctctctgg ttgaaccgct aatatgctcc
     5581 gcacgctata ccggaagcac ctttcggcgc ggagatgcaa cgattaagcc gggtaatggt
     5641 attgagcaag caactcactg gatgccgcta ccagaaccgc cgcaggaggt taattgatgg
     5701 actccttcgc gaaatatacg attattgact ggatagcatt ccttcaggtt ttgctcatct
     5761 ggttttattt ggcttatagg agtggacagt ggattgtcag tgtagcctgt agcaagggat
     5821 ggcgttggtg gaaccgaaag aataaaaaag cgctggcctt ggattcgttt tacgaagcat
     5881 tcaatcttaa cagccttcag cctggttcta tcattgtagt caccactcaa agcgacatga
     5941 cgatacaaat tcacaagcca aaggaggaag gtcgtggcta acctgcaact tgccgttaaa
     6001 ggtgaatact tcgcagatag tttcccccaa atctggggaa aagcccgaat ggcgcggctt
     6061 acagcaagat aaggcctaca tgatttgaca aatccgccag agctatcgca tactgaccgc
     6121 actagaactt caaagcggtc atccgcaccc gatagctttg cggctttttt atgcctgcaa
     6181 tttggcatag tcacatccgt acaaaggtcg ggtggagagg cgtaatacaa tacccgcaag
     6241 gggaatatgc ccggagcatc tttgaaggct ctagttgaca cccgatcacc agccactaac
     6301 tggtgattgc taactaaaac ttcaaaggag gtcatcatga ccagtcaact catccccgta
     6361 ttcaacggca ctatcgacaa cgaaacaatt cttctagtta acgctcgtga tctacactct
     6421 tttcttggtg tgggcagaat gttcgcgcac tgggttaaag aacgcattgc tgaatatgga
     6481 ttcgttgaaa gccaggacta tattttgatt tgccaaaatg ggcaaaccaa agggcgagga
     6541 ggtgatcgcc gtagcaagga ctaccaccta actctcgaca cagccaaaga actggcgatg
     6601 gttgagcgta acgaaaaagg ccgccagata cgccgatact tcatcgagtg cgaaaagaaa
     6661 cttcgcagca tgcaaccagc acagcaattc acagacgagg aaatcatcct cctctgctac
     6721 atgcaggtac agatggagaa agcacaggac atcagcaaac gcctgtatcc gatattgaag
     6781 gaactgaact catcatacgc gagcaagctg tatgacatcg cgtttgagac tttctacacg
     6841 gtgacgaaaa acagagacgc attgctcagg gaggcaacac gaattgacca gacaagcgcc
     6901 attttcgaac gggcaagacc aatgttaaaa agccttcggg cgagacaatt cgaattttaa
     6961 tatcaaagga gcttcggctc ctttttttac aggtgaaact taatgaaatt caaattcaca
     7021 ggtgaatgga atggagaacc attcaacaga gttatcgaag cggagaatat caatgactgc
     7081 tatgaccact ggatgatatg ggcgcagata gcacatgcag aagtaaccaa tattcgaatt
     7141 gaagaactga aagaacacca agccgcctga tggcggtttt ttattggaga caagaaatgt
     7201 cagatttggc tatgaaggtt ttgaaatggc aatcgactga ctatgttggc atcagtagcg
     7261 caactcttgc ctcaatcgca tgtggactga aaaagaatat ctatggtcat agcttcggtg
     7321 ctccacatga cgcagccgat ttcagacgat gcgttgcact tgttgcactt tacattgatg
     7381 agtttgaaaa cgcgctatga cgaaaaaata cactctcatt tacgcagatc ccccctggac
     7441 attccgagac aaagcaaccg atggtcaacg cggtgcaagt tttaaatatc cggtcatgag
     7501 tcttctggat atctgccgcc tcccggtatg ggaactggca gccgataatt gcctgttggc
     7561 tatgtggtgg gtgcctacac aaccacttga agcattgaag gttgtagaag cgtggggctt
     7621 tcgtctggtg acgatgaaag gattaacctg gaacaaatgc gggaaaagac agaccgacaa
     7681 gctggtcatg ggtatgggta gcaccactcg cgctaacagc gaagactgcc tttttgcagt
     7741 gaaaggaaat ctgcctgaac gcattaacgc cggaataatc cagtcattca ctgcaccgcg
     7801 ccttgatcac tcccgcaagc cggatatggc gcgagaaaaa cttgtgcaac ttcttggcga
     7861 tgttccccgg atagaactgt tcgcccgtca cacctcgcat ggatttgatg tctggggtaa
     7921 ccaatgcgga acaccatcca ttgagatggt tccgggtatt gttaaatttc tggagaaaac
     7981 caatgagcga aaaaacgacg ttgacaaagg catcaccagt tgaattaagg cagtgcctgg
     8041 aaatcgcaaa tcaacttgcc agaagtggaa tacgctttgt tccgatcccg atcacagcag
     8101 atacagaact tcatctgttt ggtgaaattc tttcccgaaa gctggatgaa ctggaaaagc
     8161 tggtagaaga agctgacacc tcaccaatcg cataacaccc ccaccgacat taaaatatca
     8221 ggagaaaaaa atgaacgccg tactcacaga attgaacaaa ttaggaaaag caacagccga
     8281 cagcatttct aaaggtctca atattgattt gaatgacgtc attgatacgc tgtggaagtt
     8341 gaaaaaccag gggatcgtaa aggtaaaaaa cggcatctgg caggcagtag agggagttgg
     8401 aagcaaaaca gatattgatt cagttcaacc agttcaacca gttcagccag ttcagccagt
     8461 tcagccagtg cagaacaaca ttataggtga cctgctacgt aaatcacgga aagaagcgcg
     8521 ccgcgccggg cggaaacaga aacgatggga gggtgcatgt aaggcgttgc aagaactgaa
     8581 taaataccgt gacttgatca acgaattgtc agagtgaacg tagcgggaat ccacgtcctt
     8641 tagggcgtgg atgatatcag gctgttgaaa tacatgtaga aaaggagcgc atactatgga
     8701 agtcaactta atgaccgaaa aagaggtttc tgatttgtta caaaaaaggc gcacagcttt
     8761 gtataatttg cgaaaaaaac acggattccc ggaaccagta cttactcatc cggcgcgata
     8821 tagtcgtcag gccgttgaac aatggcttaa agccggagga attaaccgag ctgtttagcg
     8881 tgccagaaaa ttttatcagc atacaactca tacgcatctt tctgttccga caaccagtcg
     8941 tgtttattat acacggccat aactccccca agttcatgcc ccagcatttt ttcagtgacg
     9001 tggggcataa ttccttcccc agataaattc gttaccagag aacgcctaaa atcatgcgtt
     9061 ctccattcag gaatatcaat tttctccctt aactttttca tgtacaaatt agccgacgaa
     9121 cgatcaatag gtttatctat ttcctgcccg gggaatagca catcaaaacc cgcattaagc
     9181 aatctctcga tataaggttt aacctgagtg aataccggac gccggataat attccccatt
     9241 tttgaatgct cttttggggt tgtccagacc agatcttcca tgttgaactc actggctgtc
     9301 gcgagacgta gctcagaaag tctggccccc catagcaata gcaattgatg taacaccttg
     9361 ttggaagtaa ctacttttga gttctccagc gccagccaaa tttttgccag ttccgtatag
     9421 gtaagtacac gactccctac atctggtttt ttcccaatat tcttaacact aagcttgagt
     9481 aattcgcatg atgggattaa ctggcggctg atacaccagt taataactga acgcagttgt
     9541 aacaacaaaa cacgagcctt tttcttgttg aggctttctt gtttgtcaaa gaaacgagcc
     9601 catgccgata ctgggatact ggcaactggc gagccttcaa actgtgtgta catagtgttg
     9661 tacacgacgg atctgtaaag cgtctgtgta ttgggtttca gatcagtaac atacttatcc
     9721 caccactgat ccaggcattc ttttaatgtc agttcgccgt cactgggcgc aaaataattt
     9781 ttagggttaa ctcccttcat gtacaatgcg cgcatctcac cgacgataac acgcgcgtcc
     9841 ttcagtgaag tagacggata gcgcccgaca gtaagacgta ctggcttacc attccagcga
     9901 taacggtact gaaacgtgat agttcctgct ggagtaatcc gcacacttaa cccgtcacca
     9961 tcggtgattt cagcagggcc agagtagggt tttccgtaga ggcctcgtag tttggtgtcg
    10021 ctcagtgcca atgctttata tcctgtacac aagtttataa tgtattctgt actcaatatg
    10081 tacgcattag caagtgaaca aaacactttt caacgcaaaa gaagataatc aaccatgaac
    10141 aaaaacaaac ttacaccttg attttaaaac cataaagacg gtattatcag tcatatggtg
    10201 aacaaccaaa atcaatacgc aacaatgtcc tcttagttaa atggatataa cgagcccctc
    10261 ctaagggcta attgcaggtt cgattcctgc aggggacacc atttatcagt tcgcctccat
    10321 ccgtaccagt ccgcaaaatc ccctgaatat caagccttcc gtagattcac agttcgtcat
    10381 ggttcgcgtc agatcgttga cagccgcacg ccatgacggg taaaaagtgg ataaaataat
    10441 tttacccacc ggatttttac ccatgctcac cgttaagcag attgaagcag caaagccgaa
    10501 agaaaaacca taccgcctac tcgatggtaa tggcctgtac ctttatgtcc ctgtatcagg
    10561 gaaaaaggta tggcagcttc gctacaagat tgacggtaag gagaaaatcc tgaccgtcgg
    10621 aaaatatccg cttatgactt tgcaagaggc aagggataaa gcatggactg cgaggaaaga
    10681 catctcggtt ggcatcgatc cggtaaaggc gaaaaaggct tcgtctaaca acaattcctt
    10741 tagtgcgatt tacaaggaat ggtacgagca taagaggcaa gtctggtcag ccgcctatgc
    10801 gactgaactt gcaaaaatgt ttgatgacga cattttacct atcatcggcg gccttgaaat
    10861 tcaggatatt gagccgatgc aactgctgga agtaatccgc agatttgaag atcgcggggc
    10921 aatggagcga gccaacaaag cacgcagaag atgcggcgag gttttccgtt acgctattgt
    10981 caccggaagg gctaaatata acccggcacc tgaccttgct gaagccatga agggataccg
    11041 caagaagaac ttcccgtttt tacctgccga ccagatcccg gcattcaaca aagcacttgc
    11101 aacattttca ggaagtatcg tatctctcat tgcgaccaaa gttttacgct acacagcact
    11161 aagaacgaaa gagcttcgtt ccatgcaatg gaagaacgtc gattttgaaa acaggattat
    11221 caccatcgag gccagtgtga tgaagggacg caagattcat gtggttccga tgtcggacca
    11281 ggttgttgaa cttctcacta cgctaagctc catcactaaa ccagtatcag agtttgtttt
    11341 tgccgggcgc aacgataaga agaagtcaat ctgtgagaac gctgtactgc ttgtgatcaa
    11401 acaaatcggc tatgaaggtc tggaaagcgg tcacggattc aggcatgaat tcagcacgat
    11461 tatgaacgag cacgaatggc ctgctgacgc tattgaagtg caactagcac atgccaacgg
    11521 cggatctgtg cgtgggattt acaaccatgc tcagtatctc gataagcgca gagaaatgat
    11581 gcagtggtgg gcggactggc ttgatgggaa ggtggagtag ttattacaac gattcttata
    11641 aaaaagaaaa gccttgcaca tatagcaaag cttttcactt ctcaggattt cttac
//
LOCUS       DABSYT020000127        11540 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.127, whole genome shotgun sequence.
ACCESSION   DABSYT020000127 DABSYT020000000
VERSION     DABSYT020000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.127"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            56..1242
                     /gene="ompC"
                     /locus_tag="ID725_RS20995"
                     /old_locus_tag="pgaptmp_004199"
                     /pseudo
     CDS             56..1242
                     /gene="ompC"
                     /locus_tag="ID725_RS20995"
                     /old_locus_tag="pgaptmp_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
     gene            1834..2685
                     /gene="hchA"
                     /locus_tag="ID725_RS21000"
                     /old_locus_tag="pgaptmp_004200"
     CDS             1834..2685
                     /gene="hchA"
                     /locus_tag="ID725_RS21000"
                     /old_locus_tag="pgaptmp_004200"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGV7852677.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(2793..4151)
                     /gene="hprS"
                     /locus_tag="ID725_RS21005"
                     /old_locus_tag="pgaptmp_004201"
     CDS             complement(2793..4151)
                     /gene="hprS"
                     /locus_tag="ID725_RS21005"
                     /old_locus_tag="pgaptmp_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGV7852678.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4151..4822)
                     /gene="hprR"
                     /locus_tag="ID725_RS21010"
                     /old_locus_tag="pgaptmp_004202"
     CDS             complement(4151..4822)
                     /gene="hprR"
                     /locus_tag="ID725_RS21010"
                     /old_locus_tag="pgaptmp_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGV7852679.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4955..5368
                     /gene="hiuH"
                     /locus_tag="ID725_RS21015"
                     /old_locus_tag="pgaptmp_004203"
     CDS             4955..5368
                     /gene="hiuH"
                     /locus_tag="ID725_RS21015"
                     /old_locus_tag="pgaptmp_004203"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGV7852680.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5476..6480
                     /gene="msrP"
                     /locus_tag="ID725_RS21020"
                     /old_locus_tag="pgaptmp_004204"
     CDS             5476..6480
                     /gene="msrP"
                     /locus_tag="ID725_RS21020"
                     /old_locus_tag="pgaptmp_004204"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGV7852681.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6481..7116
                     /gene="msrQ"
                     /locus_tag="ID725_RS21025"
                     /old_locus_tag="pgaptmp_004205"
     CDS             6481..7116
                     /gene="msrQ"
                     /locus_tag="ID725_RS21025"
                     /old_locus_tag="pgaptmp_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGV7852682.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            7373..8023
                     /gene="zinT"
                     /locus_tag="ID725_RS21030"
                     /old_locus_tag="pgaptmp_004206"
     CDS             7373..8023
                     /gene="zinT"
                     /locus_tag="ID725_RS21030"
                     /old_locus_tag="pgaptmp_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HGV7852683.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            8366..8896
                     /gene="yodB"
                     /locus_tag="ID725_RS21035"
                     /old_locus_tag="pgaptmp_004207"
     CDS             8366..8896
                     /gene="yodB"
                     /locus_tag="ID725_RS21035"
                     /old_locus_tag="pgaptmp_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGV7852684.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            10033..10686
                     /gene="espJ"
                     /locus_tag="ID725_RS21040"
                     /old_locus_tag="pgaptmp_004208"
     CDS             10033..10686
                     /gene="espJ"
                     /locus_tag="ID725_RS21040"
                     /old_locus_tag="pgaptmp_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HGV7852685.1"
                     /translation="MSIIKNCLSLINNALNIQKTSYSLTKMEQAGKLLNRKITPENTP
                     PMLLSYRNADLTQEKNITERVLSIFKIKRDFVAVRIQNNQFTDLKNKKIQGHQNTVAS
                     VMDWYNPQKNALGITMGTPRKSADIAKEEHRNALNFMIMEKNTFHEKILNSNDNLQKS
                     YSKTEDSSWVAASVGSLLDKGAKVYPDTSCSLRLGEPFIFTLPESVRVDVDIYPLKK"
     gene            11011..>11540
                     /gene="tccP"
                     /locus_tag="ID725_RS21045"
                     /old_locus_tag="pgaptmp_004209"
                     /gene_synonym="espF(U)"
     CDS             11011..>11540
                     /gene="tccP"
                     /locus_tag="ID725_RS21045"
                     /old_locus_tag="pgaptmp_004209"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HGV7852686.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLVQHLAEHGIQPARNMAEHIPPAPNWP
                     APPLPVQNEQSRPLPDV"
BASE COUNT         3437 a         2618 c         2312 g         3173 t
ORIGIN      
        1 gataacggca cttattgata tattcatgaa gattataatc acaagggaat acataatgaa
       61 aagaaaagtt ctggcaatgc tggtcccggc gttattagtt gctggcgcag caaatgcggc
      121 tgaaatttat aataaagatg gcaataaact ggatttgtac ggaaaagtag cgggcctgca
      181 ctacttctct gatgatgcta gcagcgatgg cgacatgtca tatgcccgta tcggtttcaa
      241 aggtgaaact cagatcgctg accaattcac tggttatggt cagtgggaat ttaacattgg
      301 cgcaaacggt cctgaaagcg acaagggtaa taccgcaacg cgtctggcat ttgcaggttt
      361 cggctttggt cagaatggta ctttcgacta tggtcgtaac tacggtgtcg tatatgacgt
      421 agaagcatgg accgatatgc tgccagaatt tggtggagat acctatgctg gcgctgacaa
      481 cttcatgaac ggtcgtgcta acagcgtagc aacctatcgt aacaatggtt tctttggtca
      541 agttgatggt ctgaactttg cactccagta tcagggtaac aacgagaaaa gcggattatt
      601 tgatcaagaa ggttcaggta acggtaatgg acgtaaactt gctaaagaga acggcgacgg
      661 ttcagtatgt ccacttccta tgactttgac tttggtttaa gtctgggcgc tgcttactct
      721 aactctgacc gtaccgacaa tcaggttcac aaaggtactc ataacactcg ttatggagat
      781 cgcttcgatg caactgctgg cggtgaaaca gcagaagcat ggaccgttgg tgctaaatac
      841 gacgctaata acgtttactt agcagctatg tatgctgaac cccgtaacat gactggttac
      901 ggtgatgctg atgccatcgc taacaaaacc caaaactttg aagttgttgc tcagtatcag
      961 ttcgacttcg gtctgcgccc gtccatcgct tatctgcaat ctaaaggtaa ggatttaggc
     1021 ggtgtaaata gtgataactt cgacagccag ggtaatcatc attacaccaa caaagatctg
     1081 gttaaatatg ttgatattgg tatgacctac tacttcaaca aaaacatgtc cacttatgtt
     1141 gattacaaaa tcaacctgct ggacaacgac gacgacttct acaaagaaaa cggcatcgct
     1201 accgatgata tcgtggctgt gggcttagtc taccagttct aatcagccaa cgcgccgcgg
     1261 cgtgttattg taaaactgaa cactagcctg agttcgtccc tcatcgaact caggcttttt
     1321 tattggcatt tcatctccat ctgcggcaca cgcaattgcc aaagcttatc gatacacccc
     1381 cttaccctgc ccctcctggc acaataacat caaccagcga ctcttcaagc caacacccaa
     1441 atgcatttct ttatgaatta atccgtaaca aacacgcttc ccatatagaa atatttgcaa
     1501 aaatagtgca tgaaataagc tttttctcta ccaatccgcg atataaatat tcatcagcac
     1561 cacaatattc cacagcacta aatctctccc cgccgccccg tacctctgat aatggtctaa
     1621 aatcattgag gccacttgcg acgaagttgc tcaataagga aaatttggcg tgaataatat
     1681 tcaacaccag tgcagcatca tcattacata cttacacccg ccgactaccg ctttcgatat
     1741 attgactcaa ttttccagcc tggttaaaaa tagcgaccag ctcagtcgca aatatagtga
     1801 ctaccctaac taagcaacaa taaggaatac actatgactg ttcaaaccag taaaaatccg
     1861 caggtcgata ttgctgaaga taatgcattc ttcccttcag aatattcgct tagccaatat
     1921 accagtcctg tctctgatct tgatggcgtg gactatccaa aaccttatcg cggtaaacat
     1981 aaaattctgg tgatcgccgc ggacgaacgt tatttgccga ccgataacgg aaaactgttc
     2041 tcgaccggta accatccgat tgaaacgttg ctgccgttgt atcatctcca tgctgcaggt
     2101 ttcgaattcg aagtggcgac catttccggt ctgatgacca agtttgaata ctgggctatg
     2161 ccgcagaaag atgaaaaagt aatgccattc tttgagcagc ataaatcgtt gttccgcaat
     2221 ccgaagaaac tcgcggatgt tgtcgccagc ctcaacgctg atagcgaata tgcagcaatc
     2281 tttgttcctg gtggtcatgg cgcacttatt ggtttacctg aaagccagga cgtagctgcc
     2341 gctttacagt gggcaatcaa aaatgaccgt tttgttatct ccctttgcca cggcccggcg
     2401 gcttttctgg cgcttcgcca cggcgataat ccactgaatg gttattccat ttgcgcattc
     2461 ccagacgccg cagacaaaca aacgccagag attggctata tgccgggtca tctcacctgg
     2521 tacttcggcg aagaactgaa gaaaatgggc atgaacatca ttaatgacga catcaccggg
     2581 cgagtacata aggaccgtaa acttctcacc ggcgacagtc cttttgcagc gaatgcgttg
     2641 ggtaaactgg cggcgcagga aatgctggca gcttacgcgg gttaatccct gttacgtttg
     2701 aatgtaagcg cataatcaat cgcagccgcg ttagtactca cgacgtggct gcgttttttc
     2761 acggttaatt tatggcgtac tgaagcccta tgttaatttc tttgcggcaa cgtaatccgg
     2821 aacacattat gcttattgag atagtgatac gtagcacttc ccccatgcaa ttcggcaatc
     2881 gctttgacta aagaaagacc aagtccctga cctacggaat gacgcgaatt atctccccgc
     2941 caaaatctac ggaagagttt ttcaggctca ttaattttcg ctccagggct ggcgatatca
     3001 atattaagat agctgttggt atcaagaaaa ctggttatat gaatacgcga tttttctggc
     3061 gaatatcgaa tagcgttaac aataagattc gataacattc gttgcagtaa aattttatcc
     3121 gcaaagattt gctgattgca ctcgacctta aagcaaatct ctttctcgtc tgaaagatat
     3181 tcaagatagt ccaacaaatt ttcgacttcc ttattgagag aaagcgagtc cagtttcacc
     3241 aaaacattgt ttttatctgc cctggcaaga aacagtatgt tctctgttaa ccgcgaaata
     3301 ttttccagct cttcaatgtt accggcaatt gttttttgat attcagcgat acttctggtt
     3361 tgactgagcg taacctgatt ctgacccagt aatgcattaa ttggcgttct aagttcatga
     3421 gcgagatcgt cagcaaactg acttagacgc tcaaaatctt tgactaaagc atgatgcatt
     3481 ttattcaacg cctgcccaag aggttttagt tctctcggta atgcgctaac ctcaacaggc
     3541 tcccggctat cgttataatt cagcgcttcc gttacaccac tcaacttttt gatctctcgt
     3601 aaccccgttc ggattaacag cggacttaat actgagcaca gtataatggc gacaatgcaa
     3661 attataatgc tattgatttt atactgttca agcatgttat gtctggctga agccaattta
     3721 gccacagtaa ccgttaatga tggcgaaact tcatcaatat taattcgtaa agcatctatc
     3781 tctgtatcat taataatgct tctgtaaatg ccagctgcgc tgattgtctc actagcaggt
     3841 atgttattta acatgtcatc actgacattt gtccggttaa caattttatt gatgccatca
     3901 ccatgaatga tcaagatatc ctgactaaca tccatcatcc ggttaaagta tacaggtaac
     3961 gtatctggat ttaccccatc aattaataac tgcttgatct gcgctgtccg gttaatgagt
     4021 gttgtatcat cgcgccattt caactcactt gcgaggccat tatagagagt ccagacaatt
     4081 ccggcgccag caacagacag cagcaatata aaaagcaagg ttaaacggac ggttatagat
     4141 aatcttttca ttattttttt accgctacga acgaatatcc catcccacgg attgtggcaa
     4201 ttagcttttc aggaaaagga tcatcaactt ttgcgcggag cctgcgaatg gcgacatcca
     4261 ccgtattggt atcactatca aagttgattc cccaaatttc actcgcaata accgttctgg
     4321 gtataatttc gccagctctg gaggccagta gccaaagtaa ctgaaactcc ttgcgcgtca
     4381 gtgtaatact gatattgtcc ctgctcacac tttgactaac agagtccatt cttaagccgc
     4441 tgatttctaa tgttgaattc aaagcgtgat gttgccttaa ttgtgcccga acccttgcca
     4501 gcaactcaga aaatgaaaaa ggttttacca gataatcatt tgctccactg tccagccctc
     4561 tgactctgtc atcgacagaa tcccttgcag taaggcaaat aacaggggtt tgctttgccg
     4621 ttcttaacgt ttgtaagatc tgccagccat ccatacccgg aagcataata tccagaatga
     4681 tcaatgcata atcatccttc agcgcaagat aaagcccatc tctgccatca gaaacggcat
     4741 caatgacata acccgcttcg gaaagcccct gcgttaccca ctcctgggtc ctttgattat
     4801 cttcaataag taaaatcttc atgaaaataa tatgccataa atcatatgtc atagaaacag
     4861 cctggttcat tacaaaattg taatgctgct gtaaggttac cctggacgct ttttcgctat
     4921 cctcaaaact cattcacatg acaaggatat aaacatgtta aagcgttatt tagtactctc
     4981 cgtagtaacg gctgcatttt cattaccttc tttggtctat gctgcacaac aaaacattct
     5041 tagcgtgcac attttgaacc agcaaacagg aaaacccgct gccgacgtga cagtcactct
     5101 tgaaaagaaa gcggacaacg gctggttaca acttaatacc gccaaaacag ataaggatgg
     5161 acgaattaag gcactgtggc ccgagcaaac tgcaactacg ggcgattacc gtgtcgtatt
     5221 taaaaccggg gactatttca agaaacaaaa tcttgaaagt ttcttccctg agatccccgt
     5281 tgaatttcat attaataaag tgaacgagca ttatcatgtg cctttattac ttagccaata
     5341 tgggtattca acctatcgtg gcagttaatg cataaatatg taaaagcaga tctctgctac
     5401 tgttagggag gacactccct gacagattaa cagtaaacgg ctcttgctgg ctaacgacaa
     5461 aaagtgtgat ggcttatgaa aaagaatcaa tttttaaaag aatcagatgt tacggccgag
     5521 tcggtattct ttatgaagcg tcggcaggtg ttaaaagcac tgggcatcag cgcagctgca
     5581 ctttctttgc ctcacgctgc gcatgccgat ctgcttagct ggtttaaagg gaacgatcgc
     5641 ccacccgccc ccgccggaaa agcgctggag ttcagcaagc ctgccgcctg gcaaaataac
     5701 ctgccactga cgccagcaga taaagtctcc ggttataaca acttctatga attcgggctg
     5761 gataaagccg atcccgccgc taatgctggt agcctgaaaa ccgatccatg gacactgaaa
     5821 atcagcggcg aagtggcaaa accattgacc ctcgatcacg atgatttaac ccgtcgcttc
     5881 ccgctggaag agcgtattta tcgtatgcgc tgcgtggaag cgtggtcgat ggtggtgccg
     5941 tggattggtt ttccgctgca caaattgctg gcgcttgccg aacccaccag caatgcgaag
     6001 tatgtcgctt tcgaaacaat ttatgcaccg gaacagatgc caggccagca ggaccgcttt
     6061 atcggcggcg ggctgaaata tccttatgtc gaaggattgc gtctcgacga agcaatgcat
     6121 ccgctcacac tgatgaccgt aggtgtttat ggcaaggcgt taccgccaca aaatggcgcg
     6181 ccggtgcgac tgattgtgcc gtggaaatat ggctttaaag ggattaaatc gatcgtcagt
     6241 attaagctga cccgcgagcg tccgccaacc acctggaatc tggcagcgcc tgacgaatac
     6301 ggtttttacg ccaacgttaa tccgcatgtt gatcacccac gctggtcaca ggctaccgaa
     6361 cgatttattg gttcaggcgg catcctcgat gtacagcgcc agccaacgct actgtttaat
     6421 ggttacgccg accaggtggc atcgctgtat cgtggcctgg atttgcggga gaatttctga
     6481 atgcgtctga cagcaaaaca ggtgacatgg ctgaaagttt gcctgcatct tgccggattg
     6541 ttgccgtttc tctggctggt ctgggcgatc aatcacggtg gactgggtgc cgatccggtg
     6601 aaagatattc agcattttac cggtcgcact gcactgaaat ttttgctggc gaccttgtta
     6661 atcacacctc tggcacgcta cgcaaaacag ccgttattga tacgcacacg ccgcctgtta
     6721 ggcttatggt gctttgcctg ggcgacactg catttaacca gttacgcatt gctggagctg
     6781 ggcgtgaata atctggcgtt attaggcaag gagttaatta cccggcctta tttaacgtta
     6841 ggaattatca gctggattat tttgcttgct ttagcgttta cttcaaccca ggcgatgcag
     6901 cgaaaactcg gcaagcactg gcaacagttg cataatttcg tctatcttgt cgcgatcctg
     6961 gccccaatac attatctgtg gtcagtgaag attatttcac cgcagccgct catctacgct
     7021 gggctggctg tactgctttt agccttacgg tataagaagt cgcgttccct ttttaaccgg
     7081 ttacgcaagc aggttcacaa caaattatct gtataacttc atattgccga caaagtacgc
     7141 gttatccctg ccggatgtgg cgcgagcgcc ctatccggcc tacgcagatc acatcaggtt
     7201 atgtatcgtt tgtcatcaac ctcaaattat caacataatt cattttattt caataagata
     7261 gataagtaca cctgagaaaa ctatgctctc atctcctaag agttgttgca ttttgtcata
     7321 tgttacaata taacattaca catcatatac aatgactctg gaggaaactg ttttggcgat
     7381 tcgtcttcac aaactggctg ttgctttagg tgtctttatt gttagcgctc ctgccttttc
     7441 gcatggtcat cactcacacg gcaaaccctt aacagaggtc gaacaaaaag ctgctaatgg
     7501 tgtttttgat gatgccaatg tacaaaaccg aacgctcagt gactgggatg gagtctggca
     7561 atccgtttat cctttactgc aaagtggcaa acttgccccc gtctttcaga agaaagcgga
     7621 tgcagataaa actaaaacat ttgctgaaat taaagattat tatcacaaag gctatgcaac
     7681 agatatcgag atgattggca ttgaggacgg cattgttgaa ttccatagaa ataatgaaac
     7741 aacatcctgt aaatatgatt acgatggata caaaatactc acctataaat caggcaagaa
     7801 aggcgttcgt tacttattcg aatgtaaaga tcctgaaagc aaagccccta aatatataca
     7861 atttagcgat catataattg caccacgaaa atcatctcat tttcacattt ttatgggtaa
     7921 tgattcacag caatctttat taaatgaaat ggaaaactgg ccaacgtatt atccatatca
     7981 gttgagtagc gaagaagtgg tcgaggaaat gatgtctcat tgagtattct catgataacg
     8041 cctcgatgcc gctttagtat gtcatcataa ctgccactgg tccatccaca aacgccactg
     8101 aacgcaagct agctacagac acgctcatca ctatgacgtg tctgtatatt aataagctaa
     8161 cccgcattga gttaaccaat aacggattcc atacacaata cggccaacag agaaagatac
     8221 ctgtgctcac gccattgctt atattggctg gttacaatgt gcactatcaa ttttttaaat
     8281 aacaaattct taaaaccaca tctgtataag gaatttttaa ggttcgtggg atagcttgac
     8341 tgtgaaaatt acaggagcta caaaaatgaa ccgattctca aaaactcaaa tttatttaca
     8401 ttggataacg ctgcttttcg ttgcaataac ctatgccgcg atggaactcc gtggctggtt
     8461 tcctaaaggt agtagtactt atctgctgat gcgagaaaca cattacaatg cgggtatatt
     8521 cgtttgggtg ttaatgtttt cacgcctgat tataaaacac cgttatagtg atccttctat
     8581 tgtgccaccg ccacctgcct ggcaaatgaa agcggcttcg ctaatgcaca tcatgcttta
     8641 tataaccttc cttgcgcttc ctctgctggg gattgctttg atggcttaca gtggaaaatc
     8701 gtggagtttc cttggtttca atgtgtctcc ctttgttacc ccaaacagcg aaattaaagc
     8761 actgataaaa aatattcacg aaacctgggc aaatataggc tactttttaa tcgtagctca
     8821 cgctggcgca gcactctttc atcactacat tcagaaagat aatactctgt tacgaatgat
     8881 gcctcgccgc aaataagtcg atcctctgac aaaggaaatg tgaaccatgg acatcaacgc
     8941 atcataaatg tttacgcaag tgcataccgc actctttcca ctatcaacaa ggaggagcag
     9001 aaaatgtgta aatcgtacaa tggtaagact tatcaaaaat acaggttaat tttggtattt
     9061 aaaccttttt tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca
     9121 actcttggaa attatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
     9181 gactcaattc ttgacccctt ctctacgcaa cttcagttcc caccaccaac tttgcggcag
     9241 tttcatagga tgaatgtcta aaagaataat ggtgagcgat aagaaaacga ctgaataact
     9301 gcacattttc gctcgaaagc ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac
     9361 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
     9421 ttaggaaaag caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     9481 ggatttgaac ccccggtaga gttgccccta ctccggtttt cgagacctat gctatgggtt
     9541 aataaaatca atatattatg tgttttattt ggaataaata ttctatattt aggattgaaa
     9601 aatcagatgg ttagcatcaa acaacctcag aatattccaa gcaaacaggt taaaaataaa
     9661 actgcacccg aacaattgat aacgacagaa aacgcctttt ccgagccacc aaaattactt
     9721 cgcatcctat ttattgctca cgttaacgcc ttgtataact cgagctctcc acggtattta
     9781 acctctcttc tgtttaacta taattccaat aaatctcgtc actgagctca atcacttcga
     9841 aagtcaagca caatatgaat ttcacataag ctatcaatga aaatcggagg tcataccatt
     9901 aatcaacaat aaaccaagtc tttctcatat aatcacttga caaacactca tgataattgt
     9961 aataaaatat ataaggctat aatacaatac tgtcaatcaa tatttcattt atatgagaaa
    10021 aaggcattta taatgtcaat tataaaaaac tgcttatcat taattaacaa tgcattaaat
    10081 atacaaaaaa catcatattc tctgaccaaa atggagcagg cagggaagtt gttaaataga
    10141 aaaataactc cagaaaatac tcctccaatg ttgttatcat acagaaatgc agatttaacg
    10201 caagaaaaaa acattacaga aagagtttta tccatattta aaataaaacg agattttgtt
    10261 gctgtaagaa tccaaaacaa tcagtttact gatttgaaaa acaaaaaaat tcaaggccat
    10321 caaaacacag ttgccagtgt catggattgg tataatcccc aaaaaaatgc tttgggtata
    10381 actatgggta caccaagaaa atcggccgat atagcaaaag aagagcacag aaatgcacta
    10441 aatttcatga taatggaaaa aaacacattt catgaaaaaa tcttaaacag caatgacaat
    10501 ttgcaaaaat catacagcaa aacagaagac agtagttggg tcgcagcttc tgtcggctct
    10561 cttctagata aaggagcaaa agtatatccc gatacatcat gctctctgag actgggagaa
    10621 ccattcatct ttacgctgcc agaatcagtt agagtcgatg ttgacatata tcctctcaaa
    10681 aaataatctt aaaggtattg acatataaga aataattaag tgtattcaca ttagactagt
    10741 cgcttaagag tagatttttg gcattttggg aagatcagcg ggacataaga gacatacaac
    10801 acaggaggaa tggctgtttt ttaatagtgc tttgaattcc gcatgaaacc tttcttagca
    10861 gtcgaaaagc aaaacatacg ttttcttttt aacaataatt gatgagtatt gcatcgagtg
    10921 tcatttatac atcgccgcat ttatttgcca ctataaaaag gctgattaca tatctatcgg
    10981 agaactaaaa ttacttataa ggtaatctgc atgattaaca atgtttcttc actttttcca
    11041 accgtcaacc gcaatattac agctgtatat aaaaaaagca gcttctctgt atcaccacag
    11101 aaaatcacat taaatcctgt aaaaatcagc tcaccttttt caccaagcag tagctccatc
    11161 agcgcaacaa ctctctttcg agccccaaac gcccattcgg catcatttca tcgacagtct
    11221 actgctgaaa gttcgttaca tcaacaactt cctaatgtga ggcagcgcct gatacaacat
    11281 cttgcagagc atggcattaa acctgcccgg agtatggctg aacatattcc tccggcacct
    11341 aactggcctg cgccaccacc gccagtacaa aatgaacaat caagacctct gcctgatgtg
    11401 gctcagcgtc tggtgcagca tcttgcagag catggcattc aaccagcccg gaatatggct
    11461 gaacatattc ctccggcacc taactggcct gcgccaccac tgccagtaca aaatgaacaa
    11521 tcaagacctc tgcctgatgt
//
LOCUS       DABSYT020000128        11461 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.128, whole genome shotgun sequence.
ACCESSION   DABSYT020000128 DABSYT020000000
VERSION     DABSYT020000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.128"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..572)
                     /locus_tag="ID725_RS21050"
                     /old_locus_tag="pgaptmp_004210"
     CDS             complement(<1..572)
                     /locus_tag="ID725_RS21050"
                     /old_locus_tag="pgaptmp_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGV7852687.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLP"
     gene            complement(574..846)
                     /locus_tag="ID725_RS21055"
                     /old_locus_tag="pgaptmp_004211"
     CDS             complement(574..846)
                     /locus_tag="ID725_RS21055"
                     /old_locus_tag="pgaptmp_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852688.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            complement(968..1312)
                     /locus_tag="ID725_RS21060"
                     /old_locus_tag="pgaptmp_004212"
     CDS             complement(968..1312)
                     /locus_tag="ID725_RS21060"
                     /old_locus_tag="pgaptmp_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HGV7852689.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(1432..1587)
                     /locus_tag="ID725_RS21065"
                     /old_locus_tag="pgaptmp_004213"
     CDS             complement(1432..1587)
                     /locus_tag="ID725_RS21065"
                     /old_locus_tag="pgaptmp_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGV7852690.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(1878..2435)
                     /locus_tag="ID725_RS21070"
                     /old_locus_tag="pgaptmp_004214"
     CDS             complement(1878..2435)
                     /locus_tag="ID725_RS21070"
                     /old_locus_tag="pgaptmp_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGV7852691.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            complement(2437..2655)
                     /locus_tag="ID725_RS21075"
                     /old_locus_tag="pgaptmp_004215"
     CDS             complement(2437..2655)
                     /locus_tag="ID725_RS21075"
                     /old_locus_tag="pgaptmp_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGV7852692.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            complement(2783..3094)
                     /locus_tag="ID725_RS21080"
                     /old_locus_tag="pgaptmp_004216"
     CDS             complement(2783..3094)
                     /locus_tag="ID725_RS21080"
                     /old_locus_tag="pgaptmp_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852693.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            complement(3087..3314)
                     /locus_tag="ID725_RS21085"
                     /old_locus_tag="pgaptmp_004217"
     CDS             complement(3087..3314)
                     /locus_tag="ID725_RS21085"
                     /old_locus_tag="pgaptmp_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852694.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            complement(3311..3592)
                     /locus_tag="ID725_RS21090"
                     /old_locus_tag="pgaptmp_004218"
     CDS             complement(3311..3592)
                     /locus_tag="ID725_RS21090"
                     /old_locus_tag="pgaptmp_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGV7852695.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            complement(3625..4341)
                     /locus_tag="ID725_RS21095"
                     /old_locus_tag="pgaptmp_004219"
     CDS             complement(3625..4341)
                     /locus_tag="ID725_RS21095"
                     /old_locus_tag="pgaptmp_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGV7852696.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQTGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASITETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            complement(4375..4836)
                     /locus_tag="ID725_RS21100"
                     /old_locus_tag="pgaptmp_004220"
     CDS             complement(4375..4836)
                     /locus_tag="ID725_RS21100"
                     /old_locus_tag="pgaptmp_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGV7852697.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(4829..5872)
                     /locus_tag="ID725_RS21105"
                     /old_locus_tag="pgaptmp_004221"
     CDS             complement(4829..5872)
                     /locus_tag="ID725_RS21105"
                     /old_locus_tag="pgaptmp_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGV7852698.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDKD
                     TDTELNPTHNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQR
                     AAVWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKG
                     GSHAASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAHGGGVFEPLDSEERGR
                     TFEALDCPDWCDD"
     gene            complement(5941..6366)
                     /locus_tag="ID725_RS21110"
                     /old_locus_tag="pgaptmp_004222"
     CDS             complement(5941..6366)
                     /locus_tag="ID725_RS21110"
                     /old_locus_tag="pgaptmp_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGV7852699.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(6350..6592)
                     /locus_tag="ID725_RS21115"
                     /old_locus_tag="pgaptmp_004223"
     CDS             complement(6350..6592)
                     /locus_tag="ID725_RS21115"
                     /old_locus_tag="pgaptmp_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGV7852700.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEDVPWKWALLIQA
                     VTAGALKYELHIPTVVIPDSDHNPPSNQGGIHENQA"
     gene            6984..7322
                     /locus_tag="ID725_RS21120"
                     /old_locus_tag="pgaptmp_004224"
     CDS             6984..7322
                     /locus_tag="ID725_RS21120"
                     /old_locus_tag="pgaptmp_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGV7852701.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRIVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            7615..7767
                     /locus_tag="ID725_RS21125"
                     /old_locus_tag="pgaptmp_004225"
     CDS             7615..7767
                     /locus_tag="ID725_RS21125"
                     /old_locus_tag="pgaptmp_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGV7852702.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            7779..8417
                     /locus_tag="ID725_RS21130"
                     /old_locus_tag="pgaptmp_004226"
     CDS             7779..8417
                     /locus_tag="ID725_RS21130"
                     /old_locus_tag="pgaptmp_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852703.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(8418..8627)
                     /locus_tag="ID725_RS21135"
                     /old_locus_tag="pgaptmp_004227"
     CDS             complement(8418..8627)
                     /locus_tag="ID725_RS21135"
                     /old_locus_tag="pgaptmp_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852704.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            9192..9380
                     /gene="dicB"
                     /locus_tag="ID725_RS21140"
                     /old_locus_tag="pgaptmp_004228"
     CDS             9192..9380
                     /gene="dicB"
                     /locus_tag="ID725_RS21140"
                     /old_locus_tag="pgaptmp_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGV7852705.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            9377..9565
                     /locus_tag="ID725_RS21145"
                     /old_locus_tag="pgaptmp_004229"
     CDS             9377..9565
                     /locus_tag="ID725_RS21145"
                     /old_locus_tag="pgaptmp_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGV7852706.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            9658..10896
                     /locus_tag="ID725_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /pseudo
     CDS             9658..10896
                     /locus_tag="ID725_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
     gene            10884..11435
                     /locus_tag="ID725_RS21155"
                     /old_locus_tag="pgaptmp_004231"
                     /pseudo
     CDS             10884..11435
                     /locus_tag="ID725_RS21155"
                     /old_locus_tag="pgaptmp_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         2924 a         2998 c         2571 g         2968 t
ORIGIN      
        1 ggtaatccca gagagcgacg cgatactgcc tcaggtaatg cgtccaccac ctgattgcag
       61 actgcccacc aggataattc agccagcgat aattcccgct cctgcgtgcc attcattaca
      121 tggcgtatga cgtcaatcat ccaggctgtc agattttgtt gagtaagttg ctcaagtgat
      181 tcggaggtct ggtcgcgcag ctggttgtcg cagtgccagc acaacaccat tgcgccagca
      241 ccgtaactgt gaataacggt ttcgctgtga tgataatcgc cgtgtggcca ctggcaggat
      301 ttcacgtgac gtaataacca gtcagacagt gcaccagcgc cgccagctgc acgaatcacc
      361 cgctcatcgc tgaaaaatgg cagcagtgat ttatcttccg ccagcggctg gcgaacagca
      421 ggaaccgctc cagatggcag cgcccgcatg tttttcggtt ccggctccac cagcaccctg
      481 ccgcgatgaa aaactggcaa tgattcacga ccgggcttaa ggaccaccag cccgagttcc
      541 ggaactagaa caggtcgaag taatacccgc acattacctc cagacgcgct gacggtagca
      601 ggcatgtgtc cgtggcagat gtgcacgaac aggaagatat acagaaacgg tccaggtcag
      661 acgatcagcg ttcagactcc gctccacacg gacaccgcga cgcagatacg cctcttgaag
      721 catatctgcc tcatcgatcg tacagaacag atagtgaaac cagccatact gaggcgcacg
      781 aaaacgcctc ccctgcttaa tttccgggtc ggcttcagaa ttgtgggatt ttatgtgttg
      841 tgtcatcgga ttctccggtg acagcaggtg tcagttgttc aggctgactg cgcgaattgt
      901 aaggcaatac gccggaatgt acaaacagaa aacccgtcag taagacgggc ttaacaagca
      961 ggggcggtta ctttaataat ttcagtgcct ttacatcaac ttcaacactg ctcaggtctt
     1021 tatcaatttc accctcaatt cttactttgt ctttcggaga aacattctgc ccggcccata
     1081 cgctgtcatc aatatccgtg acaattgtcc cgctattgtc acgaaactca taacgttcat
     1141 cacccacttt tttaacgatg ctcccttcaa ggataaccca tgcatcatcc ttcagttctt
     1201 ttgcctgcgc tactgttgaa cgctctgctt ctggcccctg gaaaccaccc tgctgtgcaa
     1261 aagcgccaaa agacacaccg gaaataagtg ctgcaatcaa tacctttttc attcatagtc
     1321 ctctttcaga gatgaacatt caaacagcat tttcagtatg gtaaagcgcg ggtgcgttga
     1381 ggatgcctga cacatcagag gtggcgggag attactcccc cgcctggtct cttacttctc
     1441 agattcgtag tctacgaaga cagcgacctc cgtctgaccg gttcggattc gcacctcgca
     1501 gaggtctttc ctcgttacca gtgccgtcac tatgacggtt aaacagatga cgatcagggc
     1561 gattaacatc gccttttgct gcttcatagc ctgcttctcc ttgcctttcg gcacgtaaga
     1621 ggctaaccta catgtgttca gcatggattg agcctcagat taatgttaag cgtcttgcag
     1681 gacgcgtaat gttaactggg gcttttctct atctgccgtt ggtgttcatg cccgaggcag
     1741 atagcctcaa gcacccgcag caattctact taactatcct tttcccgcaa accgttttta
     1801 tccccagcgg caaatcgaat acaccaccag cgccaccgcc atcgcaattc ctaccgttgt
     1861 gaatgcttca ggccaggtca ttgattcacc tcctgcggcg gttctggtag cgacatccag
     1921 tgggttgctt gcctaagatc attacccgga ctaactgcta tacctccgcg ccggaacgtg
     1981 cctccgagat agcgtgcgga atatattaat ggcccagcct cgctatcgat attcatcgaa
     2041 ataagcacgt tctggctctt ttcaggcatt cgctcagtac agcttatcca gccatccaga
     2101 gttatcggag atctggttga cgtttccgag atttcccgaa aattgttggt tgacgaattc
     2161 ttattttccc gaaagtttcc ggcctgaagc atggcgacgc ggcaggcgtt ccagccatca
     2221 gcatatgttt tagttacacc gtcgagatgg caggtaagca aatccatttc atcaggcact
     2281 accattgcta tcggctctgc ttccagtgat gccagcgcga tacgaaacac attagccagc
     2341 aggctgtctg aagacttgtt atcgtgcgcc gagtcgctca ggaagcctgt gatgtatgat
     2401 ttaatctccg cgcgttctct ggtaatagtg ctcatatcag ttttccttat acggattaat
     2461 tttattgtgc agtgcgctga atgattccca tgtcacatcg gtatatagct caataactgg
     2521 ttcaaatgtc cttccaatta tccagaccag taatagcggg gatatcggta tcatcaacac
     2581 tataaacaga atgaaaaaca gaaactctgt tgttctgctc tttcgtgggt aatttttttc
     2641 taaataatgt ttcatttctt accgcccttt cgggcggcct cctgatgttc tgagggtgca
     2701 ggaatccctc cggttaagga tttaataaaa atcgtttctg atttaaatct tcagtattta
     2761 gttgttagtt ggtttatcgc ctttatgctt cagccttatt tcgcaaccag acacaaaccg
     2821 ggccatcttc cgtatcatga atggaaccaa taaaccagcc atcgccctct ggtcgttccg
     2881 gttcccatgc agaaatatca gcatcacacg catcaaggtc agcacatcct tcatctctga
     2941 agcagaggac gtattgaaga ttattttcct ccatccaggc gttaaactct tccgttgaaa
     3001 tatattcccg accatcacag aatttttcat attcaggatg tgtccagtag ccatatccgt
     3061 cacgaactac cggcatttct ttaatttcac tcactgttaa cctcctgcaa cgctacacga
     3121 tacgccttct ttatccacgc cttactgcca tataatttcg tcttcataat aaacacacct
     3181 gcacgactcg ccgatatccc cggacaggtt aacagcacag catccaccac acggttatgc
     3241 ttccgaaact ccattacagt actgctgata accacctgcc ccaccgggcc gtaatcctga
     3301 tacaggattt tcacgcagac accctcctgt cgaaataaac gtagttattc actatgcgca
     3361 gcggcatgcc taattttctg gcaatttccc ttctttgcat gcctctctga tgcagttgcc
     3421 gcgccagctc aatatcacgc tgagaatatt ttgccgacgg gtgaaaatca ccacgtaaca
     3481 tcatgctgat gcccagctcc cgtgccttcg ttctcactgc cgcttcagtt cgtccgataa
     3541 gtgcgccaac gctttttacc ttcatcgttc ccgcacactg ccggagtatc atgatttccg
     3601 cccggcacca cgtcttccac ccactcaccg ctgctgttct ctggtggcgg taatatcccg
     3661 gagaatatcc cggcacttgt tcagctcccg cagcgcggcg cagactcgct cccacttctg
     3721 aacctgacct tttgcccggc gcagctcgcg gttagccaca tgcagcgatg gtaaaatcag
     3781 accatccgga tgctttctgg tgaacgacgg ctgtgactgc actgtgaccg ccacactttc
     3841 cgtttttatt tcttcctgtg tttccgcttc cccgactggt aacgcaacac ctgctggctg
     3901 aggaaaggct ttaccatccg tttccgttat ggatgcggct ttcggctctg ccggtaaatc
     3961 agcgcccggt atgcagtacc gaaatttacc gccctgattc acgcgaatca gacgcccttt
     4021 gctgattgcc atggccagtg atgaattcgc ccggcgggag gtaatcccga acatcagtgc
     4081 cagctcatcc gccgtttgtg ggccatgatg ttcaatcgcc tcagtcagca tttgcgctgt
     4141 cactttcggt accggtgaca ctggttcact ttcaccagtc tgagtcagcc accacatcga
     4201 ccccttgtta tccgcttcac cacggcgctt cagtttccac agttcgttga cagcatcttc
     4261 gcggctgatt ccaaggcgcg atgccactac ctgtgaagag gcttttttca gtgctttcag
     4321 tgcgtcaaat acggtttcca ttaatatttc ctccgacaaa atcgtttctc agattcaaat
     4381 aaaaccagct gccttccggc gttcgtattc ctgtttcagc cgttcaattg gcgttggccc
     4441 ttgcgggtgt ttcgcccctt ccagttgtcg tcgcactggc ggaacactca tcccgttacc
     4501 aacatgcttt gcccatttcg tcagttgccg ttccgcaagt cgttttaact caccttgcgt
     4561 catctggcgc tcaatccctc tggtacgcat ttcgaggcag atgtggtaca gcacaggctg
     4621 tggccacggg tatttatcac tcccgtcgta tcgccaggat tcattgcgcc agcgccggta
     4681 ctcttccatc acggcatcca ccgtaagacc aaatggattt gccccactct ccgaaatcag
     4741 cgcaacaaac tcagccaggt ccgggggcca cgtttcaccc gcccggcagc ggtccatgca
     4801 ctgacggcac accagacgga tttgctgttc agtcatcgca ccaatctggg caatccagag
     4861 cttcgaaggt gcggccccgt tcttctgagt ccagcggttc gaatacacct cccccatgag
     4921 ctcccacagc ttccaggccg tttccgttgc tgataaatcc gttgtcacgt tcccactgtt
     4981 cgcgtgcagc ccggatttcc tgaactgccc gtgatgccgt gccacctgat gctgcatggc
     5041 ttcccccctt gctgactggt tttacctgtg ccctgacgtg ctgcacgtgg cgggcaaatt
     5101 tctgctccca ctgaacctgc gtgaaaacct tcccctccgc catccagtaa tcccggaatg
     5161 cggcaagctc agcaggtgta aattccggct caggcagagc cataccccac actgctgccc
     5221 gttgtcgaaa atccggcgac ggctgccaga cagtagtcat cgaaaatttc ccgatcggtt
     5281 cgctcaggcc gtccaggtat tcaggttcgg ctgtctgcaa cggcgcacca ttcgactcac
     5341 tggtcggaat actctcgcgc gcgttatgtg tggggtttaa ttctgtatct gtatctttat
     5401 ctgtatcttt atctgtcgtg acttgtcgtg acagatgcgt gacacgtcgt gactcatcgt
     5461 gacaatcagc attatgtttc cgcagctttt cccgctcccg ctgcgctctc ttgcgctctg
     5521 ccggggattt tgccgtttgc gaaacgttac cattgtcctc tttcagcacc tgacgttttt
     5581 cccatccgga aataaggtca ccatccagaa cccgcccctg cattgcatgc aaaattgaat
     5641 caattacgtc ttccgtcaca tcaagcgcac ttgctaaatc ttccgtcgtg acatcaatgt
     5701 gaccacgtag tgacacgccg tgacatgtcg tgacatttcg tgacgcgctc accagaagat
     5761 ggatatacac tgccatcact gttgcgattg gctgtcctga taccctggca atcgttcgcc
     5821 acttggggtc atttggcatg tcatgccaca atctgagcca ggcattagcc atactcacct
     5881 cttctgatac cgaactttac cctcgaacat ccggaagaaa tccggcatga atattgttgg
     5941 tcaatgcacg acaacagcat taccaggctg acccccactg ttagtcaggg tgccccaggc
     6001 gatcgccgct gcgacaaaat catccacatc tttcaccagc cgatccctcc gttcgacgat
     6061 ctcacggtaa tattcagagc tgtgactgcg catacgggcc accagcagag gcggcattgc
     6121 cttttcgatc gccggtaaca gagcctgcat tttttcaact gcatcagggg tgtctttctc
     6181 tatccagcgg aaaattttct gggtattgcg agccagggct tccggatggc tgtcgtcgta
     6241 cagttcagga aacgtcatac ccaactcaaa ataagcctgg gttattccag ctgccggaac
     6301 tttttcgcca tcaggacgcg cccaggcatt catcgccatg cggatgtgtt catgcttgat
     6361 tttcatgaat ccccccttgg ttagaaggcg gattatgatc agaatcggga atgacaaccg
     6421 tcggtatgtg taactcatat ttgagcgccc cggcagtgac tgcctgaatt agcaacgccc
     6481 atttccacgg aacgtcttcc ccccacatgc tgactgtggt ttttgacgtt cctagagcgg
     6541 cggctgtttt aacaactccg ccaaaatagc ctaatacttc tgattttttc atgagtcgct
     6601 ccataaaact gaacgccaaa agtttaataa tcaaaaccaa agaaagtcaa gaaacaaaac
     6661 catctgtgtt ttaaaatcaa aacatgaaca agcaaacaat atctgaacgc ataacccaac
     6721 gtatgcatgc gctaaacttg aaaggcaaag accttgtcaa tgccactggc gcatcaaaag
     6781 gctccgtaag tcaatggatg aacggtggag gagcgccgtc ctcgcgttac ataagttcac
     6841 tggcaaagat attgaaagta aacgaaaatt ggcttcttaa tggaggagag ttaaatacag
     6901 gtgattcgct tgatctatct ttaccgccga taaaaacggt tccgctacta tcacttcagc
     6961 aggcagcaag ctggagtgat tatatgaaaa attcctcaat aacctcttgt gtgcagcttg
     7021 tcggagaaat cccggccaat acctttgctg ttgttctaga gagtgacagt atgtcgacat
     7081 ctggtggtgg agtttccatc ccaaatggtt caacagtttt tgttgatccc gatcgaatcg
     7141 tacaaccagg aaatattgtc cttgccttac ccaaagggac cacaacgcct gtcattcgca
     7201 aactggagat agaagggccg gatattcttt tagtccccac gaatcctcgc tacccttcaa
     7261 ttatgctgga tgatctatct tgcatattgg gagtatgctt taaaattcaa caagatattt
     7321 aaccaacctc atctatttga ttaactgtat gccatcgtag tgatggcgta acagctgcct
     7381 gcttaaaatg ttttgataaa aaaacattga ccttaaatgt tcgtttttct aaacttcatt
     7441 cattccctca ccccgcccca cagaatgcag ggcaatactt cgagttacca ggcagtggtc
     7501 aggggttaag tagccagccc gaggcgtaag aacatgacgg cagggttcaa ctttaataac
     7561 tatgcagcag gtttttgttc cgctaccccg gcgttaaggg gaaatgaggt caacatggat
     7621 acgctcaatc ttggcaacaa cgaatctctg gtatgtggcg tgttccccaa ccaggacggt
     7681 acgttcaccg cgatgacgta taccaaaagc aaaacgttta aaactgaagc tggcgcgcgt
     7741 cgctggttag ccagaaacac tgactgatga ggttgacgat ggaatttaaa gatttacctc
     7801 cttcaatcca ggagattgca gcacacacac ttcgtcatcg tctgaacgaa cttgaattgg
     7861 aatcggtaac gaaaaaagac actgataata tggctcgtaa tgtgcgcgat gcgtttaccg
     7921 gattgtattt ctgtgcgtct ataaataaac acgactcaga gagtgtggca aataaaattg
     7981 cagaaacgac agcgcaaaac atcaatacga aaccaacgga agaagaaatt gatcagtttg
     8041 ctcatgatgc tggtttaaaa aacaagaaag aaaaatcgcc atatgcgggg aacatgtttg
     8101 tttatgacaa tctcatcaga attcgtggcg aaattccggc ggaatacctg gcaagagtcc
     8161 atcaggcatt gcttaaaaat ttggaaacag aattatttga tggcaacact aacggtttct
     8221 tcatggtatc aggccttgag aaagactggg atgcagaaaa acgctggaat gttgctacat
     8281 ggttattcag taacagagcc gctgcactgg aagcttcggc atgtatttgc ggcctgttct
     8341 taacagacca caaatataat ctggatgtgt acagttatat ttacgctgaa cacggtccac
     8401 tctggattga ctggtaatta taaggaaaca ccagcagggc cgcggcgacc aacagaacga
     8461 ttaaaatcaa taatgccatt ataaaggaca ttatttaatt tatcgtcgaa tgctgattct
     8521 gtgagcctca actctgaatg agtttttaat aaccctgatt gcctgagttg atttaccagg
     8581 cattcaatct gtttttcaat aagcggattt ctttttttgt ttggcatttt atcctccatt
     8641 gaggttctgg gttaaaaatg gagaccaaca cgctgtcacg tgtggtcgtg cgccggacac
     8701 ggataagaat ccggtactga cagtttactg aaaggatatt tccctgaaaa gtcagtgcat
     8761 aacgcgaaag cgtacggcga agctctttcc cttagaaggc ttgtcgttag atttcttcga
     8821 acgtgcgctt ccggttgtgg cactccgcga aatggcgcgg cggtaagtat ggcggggtta
     8881 ttccttcccc attgaggaca ccgggttgtc aggttgacca tacgcttaag tgacaacccc
     8941 gctgcaacgc cctctgttat caattttctg gtgacgtttg gcggtatcag ttttactccg
     9001 tggctgctct gccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc ggctaagcgc
     9061 acgcggaaca gttaaaacca aaaacagtgt tatgggtgga ttctctgtat ccggcgttaa
     9121 ttgttaactg gttaacgtca cctggaggca ccaggcactg catcacaaaa ttcattgttg
     9181 aggacgcgat aatggaaacg ttattaccaa acgttaatac gtctgaaggt tgttttgata
     9241 ttggtgttct gctcagtaac cgggagttta ctgaagatgc cattaacatg aggaaatatg
     9301 agccttatct gctcaatgat aattccatac tttcccgaat tgctcttctt gaacttggta
     9361 ttttcggaga acgtcaatga cttcagcatt tgcactgatg atgacggttt ttcttataac
     9421 gggtgaatca cagaatgtga ttaccggaat ttatgccagt aaagaatcct gcctccaggc
     9481 aagagacgag caaaaaattt ctggtgaatg cctcccggta aaaaaagtat cgctgtacct
     9541 gaataacgaa acaccggctg gataaccctc cagccatatt aacaccatac caacggatta
     9601 aaaatgccag caatggcagg gatttgttca cccttaaatc tgtaatgagg tttatcaatg
     9661 agcactgata aagaagaatt tgcgctatat tgcgaagcaa aaaatgacaa agtcagaaaa
     9721 cgtctgggaa ttaaaggtgg tttttactgg actacagcaa aaaaattatc tgttgccatc
     9781 tcccgctgca ttaccgcaat ggatgacaac gattatgatg aagacgactt taaaaaaccc
     9841 gttcgcgtcc atttacccgt tgtgaatgac cttccacctg aaggcgtgtt tgataccgaa
     9901 ttctgcaacc gatacgaaaa aggcggggaa gatggcatta caatggtatt tatcgcgccc
     9961 tctccctccg tgcaggagaa accagccagt actgacaata ccaacgtcaa cggcgaagac
    10021 atgacggaga ttgaggagaa tatgctcctg ccggtttctg gtcaggagct gcccattcgc
    10081 tggcttgcgc aacacggcag cgaaaaacca gtaacgcacg ttgcacggga agaacttcag
    10141 gcattacata tagcacgggc ggaagaactg ccggctgtta ctgccctggc catttctcac
    10201 aaaacaaagc tgctcgaccc gctggagatt cgcgaccttc acaaactggt acgcgacact
    10261 gacaaagttt tccctaatcc cggtaattca gacctgggac tgataactgc ttttttcgag
    10321 gcatacctgg acgctgacta cactgatcgg ggtctgctga caaaagagtg gatgaaagga
    10381 aatcgtgttt cgcgtatcac ccgtacggct tccggtgcaa atgccggtgg cgggaacaaa
    10441 accgatcgca atccgaattt agtacacacc ttcgatacgc tggatgtgga gattgcagca
    10501 gccacacttc cgatggattt taatatttat gaaattccgg gcagcgttta tcgtcgcgca
    10561 aaagaaatcg tcctgaaaag agaaagtccg ttcaaagaat ggtccgcagc acttcgcgca
    10621 accccgggta ttctggacta ttcccgcgcc gctatttttg cacttatccg gagcgctcac
    10681 cctgagtttt atcactaccc gggacgcctt caggggtata tcaacgccta cttaacggag
    10741 actgatcacg agaaccccag caaggaaact ctcactgctg cacgacatac accggaaaaa
    10801 gatatcctgg aagaagttaa ccgcgaactg tctgctaagc aggaaacaga agaagaaaat
    10861 gatgaagaaa aaccgcaacc atcttgcgca atggcagaat gataatcaga agaaatctgg
    10921 caaaagaccg ttttacagta ccaaaaacac aggcgggaac caatcgtgtg attcacctta
    10981 ttaagcccgc aatcgacgct ctccggagtc agatggcact aacgagactg agcaaagagc
    11041 atatcatcga tgttcacctc agagaattcg gcagaacaga gaaacaaaaa tgtacctttg
    11101 tttttcaacc tgaagtgtca gcgaaagtaa aaaattatgg cgaccatttt accgttgact
    11161 caataaggca gatgtgggac gcagcggtaa agcgtgccgg aatccgccat cgcaaatcgt
    11221 atcagtcgag acacacttat gcctgctggt cgttaacagc aggagctaac ccggcattca
    11281 ttgcaaacca gatgggccat gcagatgcgc aaatggtgtt tcaggtgtac gggaaatgga
    11341 tgtcagaaaa taataatgcg caggtaacgc tgttaaatac acagttaagc gagtttgccc
    11401 caaccatgcc ccataacgaa gcgatgaaaa gttaattttt tatttatcaa ttagttagat
    11461 t
//
LOCUS       DABSYT020000129        11231 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.129, whole genome shotgun sequence.
ACCESSION   DABSYT020000129 DABSYT020000000
VERSION     DABSYT020000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.129"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            253..2676
                     /gene="pgaA"
                     /locus_tag="ID725_RS21160"
                     /old_locus_tag="pgaptmp_004232"
     CDS             253..2676
                     /gene="pgaA"
                     /locus_tag="ID725_RS21160"
                     /old_locus_tag="pgaptmp_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HGV7852707.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2685..4703
                     /gene="pgaB"
                     /locus_tag="ID725_RS21165"
                     /old_locus_tag="pgaptmp_004233"
     CDS             2685..4703
                     /gene="pgaB"
                     /locus_tag="ID725_RS21165"
                     /old_locus_tag="pgaptmp_004233"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HGV7852708.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4696..6021
                     /gene="pgaC"
                     /locus_tag="ID725_RS21170"
                     /old_locus_tag="pgaptmp_004234"
     CDS             4696..6021
                     /gene="pgaC"
                     /locus_tag="ID725_RS21170"
                     /old_locus_tag="pgaptmp_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HGV7852709.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6023..6436
                     /gene="pgaD"
                     /locus_tag="ID725_RS21175"
                     /old_locus_tag="pgaptmp_004235"
     CDS             6023..6436
                     /gene="pgaD"
                     /locus_tag="ID725_RS21175"
                     /old_locus_tag="pgaptmp_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HGV7852710.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6486..7274)
                     /gene="phoH"
                     /locus_tag="ID725_RS21180"
                     /old_locus_tag="pgaptmp_004236"
     CDS             complement(6486..7274)
                     /gene="phoH"
                     /locus_tag="ID725_RS21180"
                     /old_locus_tag="pgaptmp_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGV7852711.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7894..9165)
                     /gene="efeB"
                     /locus_tag="ID725_RS21185"
                     /old_locus_tag="pgaptmp_004237"
     CDS             complement(7894..9165)
                     /gene="efeB"
                     /locus_tag="ID725_RS21185"
                     /old_locus_tag="pgaptmp_004237"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGV7852712.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9171..10298)
                     /gene="efeO"
                     /locus_tag="ID725_RS21190"
                     /old_locus_tag="pgaptmp_004238"
     CDS             complement(9171..10298)
                     /gene="efeO"
                     /locus_tag="ID725_RS21190"
                     /old_locus_tag="pgaptmp_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGV7852713.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10356..11186)
                     /gene="efeU"
                     /locus_tag="ID725_RS21195"
                     /old_locus_tag="pgaptmp_004239"
     CDS             complement(10356..11186)
                     /gene="efeU"
                     /locus_tag="ID725_RS21195"
                     /old_locus_tag="pgaptmp_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGV7852714.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         2932 a         2788 c         2640 g         2871 t
ORIGIN      
        1 cctaatttat tttgaaaaag gcatcgggat ttatgccgta ttcctgaaga tcctcatcat
       61 tggaatggat tttcgggcga gaaaaggatt ttatatggac actctgctca tcatttcttc
      121 ttctcatcat caacaattca cgtctctctt ccgcgtttaa taacggatta tgaggtgcaa
      181 aaatatcttt cttttcagtt acctgtaatt agatacagag agagattttg gcaatacatg
      241 gagtaataca ggatgtattc aagtagcaga aaaaggtgcc cgaaaaccaa atgggctttg
      301 aaacttctta ctgccgcatt tttagcagcg agtcccgcgg cgaagagtgc tgttaataac
      361 gcctatgatg cattgattat tgaagctcgc aagggtaata ctcagccagc tttgtcatgg
      421 tttgcactaa aatcagcact cagcaataac caaattgctg actggttaca gattgcctta
      481 tgggccgggc aagataaaca ggttattacc gtttacaacc gctaccgtca tcagcaatta
      541 ccagcgcgtg gttatgcagc tgtcgccgtc gcttatcgta acctgcaaca atggcaaaac
      601 tcgcttacac tgtggcaaaa ggcgctctct ctggagccgc aaaataagga ttatcaacgg
      661 ggacaaattt taaccctggc agatgctggt cactatgata ctgcgctggt taaacttaag
      721 cagcttaact ctggagcacc ggacaaagcc aatttactcg cagaagccta tatctataaa
      781 ctggcggggc gtcatcagga tgaattacgg gcgatgacag agtcattacc tgaaaatgca
      841 tctacgcaac aatatcccac agaatacgtg caggcattac gtaataatca acttgctgcc
      901 gcgattgacg atgccaattt aacgccagat attcgcgctg atattcatgc cgaactggtc
      961 agactgtcgt ttatgcctac gcgcagtgaa agtgaacgtt atgccattgc cgatcgcgcc
     1021 ctcgcccaat acgctgcatt agaaattctg tggcacgata acccagaccg tactgcccag
     1081 taccagcgta ttcaggttga tcatcttggc gcgttattaa ctcgcgatcg ttataaagac
     1141 gttatttctc actatcagcg attaaaaaag acggggcaaa ttattccgcc ctgggggcaa
     1201 tattgggttg catcggctta tctcaaagat catcagccga aaaaagcaca gtcaataatg
     1261 accgagctct tttatcacaa ggagaccatt gccccggatt tatccgatga agaacttgcg
     1321 gatctctttt acagccacct ggagagtgaa aattatccgg gcgcgctaac tgtcacccaa
     1381 cataccatta atacttcgcc gcctttcctt cggttaatgg gcacgcctac gagcatcccg
     1441 aatgatacct ggttacaggg gcattcgttt ctctcaaccg tagcaaaata tagtaatgat
     1501 cttcctcagg ctgaaatgac agccagagag cttgcttata acgcaccagg aaatcaggga
     1561 ctgcgcattg attacgcgag tgtgttacaa gcccgcggtt ggcctcgtgc agcagaaaat
     1621 gaattaaaaa aagcagaagt gatcgagcca cgtaatatta atctggaggt tgaacaagcc
     1681 tggacagcat taacgttaca agaatggcag caggcagctg tcttaacgca cgatgttgtc
     1741 gaacgtgaac cgcaagatcc cggcgttgta cgattaaaac gtgcggttga tgtacataat
     1801 cttgcagagc ttcgtatcgc tggctcaaca ggaattgatg ccgaaggccc ggatagtggt
     1861 aaacatgatg tcgacttaac caccatcgtt tattcaccac cgctgaagga taactggcgc
     1921 ggttttgctg gattcggtta tgccgatgga caatttagcg aaggaaaagg gattgttcgc
     1981 gactggcttg cgggtgttga gtggcggtca cgtaatatct ggctcgaggc agagtacgct
     2041 gaacgcgttt tcaatcatga gcataaaccc ggcgcgcgcc tgtctggctg gtatgatttt
     2101 aatgataact ggcgtattgg ttcgcaactg gaacgcctct ctcaccgcgt tccattacgg
     2161 gcaatgaaaa atggtgttac aggcaacagt gctcaggctt atgttcgctg gtatcaaaat
     2221 gagcggcgta agtacggtgt ctcctgggct ttcactgatt tttccgacag taaccagcgt
     2281 catgaagtct cacttgaggg tcaggaacgc atctggtctt caccatattt gattgtcgat
     2341 ttcctaccca gtctgtatta cgaacaaaat acagaacacg ataccccata ctacaaccct
     2401 ataaaaacgt tcgatattgt tccggcattt gaggcaagcc atttgttatg gcgaagctat
     2461 gaaaatagct gggagcaaat attcagcgca ggtgttggtg cctcctggca aaaacattat
     2521 ggcacggatg tcgtcaccca actcggctac gggcaacgca ttagctggaa tgacgtgatt
     2581 gatgctggcg caacgctacg ctgggaaaaa cgaccttatg acggtgacag agaacacaac
     2641 ttatacgttg aattcgatat gacattcaga ttttaaggat aaatatgtta cgtaatggaa
     2701 ataaatatct cctgatgctg gtgagtataa ttatgctcac cgcgtgcatt agccagtcaa
     2761 gaacatcatt tataccgcca caggatcgca aatctttact cgccgagcaa ccgtggccgc
     2821 ataatggttt tgtagcgatt tcatggcata acgttgaaga cgaagctgcc gaccagcgtt
     2881 ttatgtcagt gcggacatca gcactgcgtg aacaatttgc ctggctgcgc gagaacggtt
     2941 atcaaccggt cagtattgct caaattcgtg aagcacatcg aggaggaaaa ccgctaccgg
     3001 aaaaagctgt agtgctgact tttgatgacg gctaccagag tttttatacc cgcgtcttcc
     3061 caattcttca ggccttccag tggcctgctg tatgggcccc cgtcggcagt tgggtcgata
     3121 cgccagcgga taaacaagta aaatttggcg atgagttggt cgatcgagaa tattttgcca
     3181 cgtggcaaca agtgcgagaa gttgcgcgtt cccggctcgt tgagctcgct tctcatacat
     3241 ggaattctca ctacggtatt caggctaatg ccaccggcag cttattgcct gtatatgtaa
     3301 atcgtgcata ttttactgac cacgcacggt atgaaaccgc agcagaatac cgggaaagaa
     3361 ttcgtctgga tgctgtaaaa atgacggaat acctgcgtac aaaggttgag gtaaatccac
     3421 acgtttttat ttggccttat ggcgaagcga atggcatagc gatagaggaa ttaaaaaaac
     3481 tcggttatga catgttcttc acccttgaat caggtttggc aaatgcgtcg caattggatt
     3541 ccattccgcg ggtattaatc gccaataatc cctcattaaa agagtttgcc cagcaaatta
     3601 ttaccgtaca ggaaaaatca ccacaacgga taatgcatat cgatcttgat tacgtttatg
     3661 acgaaaacct ccagcaaatg gatcgcaata ttgatgtgct aattcagcgg gtgaaagata
     3721 tgcaaatatc aaccgtgtat ttgcaggcat ttgctgatcc cgatggtgat gggctggtca
     3781 aagaggtctg gtttccaaat cgtttgctac caatgaaagc agatattttt agtcgggttg
     3841 cctggcaatt acgtacccgc tcaggtgtaa acatctatgc gtggatgccg gtattaagct
     3901 gggatttaga tcccacatta acgcgagtaa aatacttacc aacaggggag aaaaaagcac
     3961 aaattcatcc tgaacaatat caccgtctct ctcctttcga tgacagagtc agagcacaag
     4021 ttggcatgtt atatgaagat cttgccggac atgctgcttt tgatggcata ttgttccacg
     4081 atgatgcttt gctttcagat tatgaagatg ccagtgcacc ggctatcacg gcttatcagc
     4141 aagcaggctt tagcgggagt ctgagcgaaa ttcgacaaaa cccggagcaa tttaaacagt
     4201 gggcccgctt taaaagtcgt gcgttaactg acttcacttt agaacttagt gcgcgcgtaa
     4261 aagccattcg cggtccacat attaaaactg cacgaaatat ttttgcactt ccggtaatac
     4321 aacctgaaag tgaagcctgg tttgcacaga attatgctga tttcctaaaa agctatgact
     4381 ggaccgctat tatggctatg ccttatctgg aaggtgtcgc agaaaaatcg gctgaccaat
     4441 ggttaataca attgaccaat caaattaaaa acatccctca ggctaaagac aaatctattt
     4501 tagaattaca ggcacaaaac tggcagaaaa atggtcagca tcaggctatt tcttcgcaac
     4561 aactcgctca ctggatgagc ctattacaac tgaatggagt gaaaaactat ggttattatc
     4621 ccgacaattt tctgcataac caacctgaaa tagaccttat tcgtcctgag ttttcaacag
     4681 cctggtatcc gaaaaatgat taatcgcatc gtatcgtttt ttatattatg tctggtgtta
     4741 tgcatacccc tatgcgtagc gtactttcac tctggtgaac tgatgatgag gttcgttttc
     4801 ttctggccgt tttttatgtc cattatgtgg attgttggcg gcgtctattt ctgggtctat
     4861 cgtgaacgcc gctggccgtg gggagaaaac gcaccagctc cccagttgaa agataatccg
     4921 tctatctcca ttatcattcc ctgttttaat gaggagaaaa acgttgagga aaccatacac
     4981 gccgctttag cacagcgtta tgagaacatt gaagttattg ccgtaaatga cggttcaaca
     5041 gataaaaccc gtgccatcct ggatcgcatg gctgcacaaa ttccccattt gcgggtcatt
     5101 catctggcgc aaaaccaggg gaaagccatt gcgcttaaaa ccggagctgc cgcggcgaaa
     5161 agtgaatatc tggtgtgcat tgatggcgat gcgttattag accgcgatgc ggcggcatat
     5221 attgtggaac cgatgttgta caacccgcgt gtgggtgccg taaccggtaa tcctcgtatt
     5281 cgaacacgtt ctaccctggt gggtaaaatt caggttggcg agtattcctc aattattggt
     5341 ttgatcaagc gaacccagcg tatctatgga aacgtattta ccgtttccgg tgttattgcc
     5401 gcatttcgtc gcagcgccct ggcagaagtg ggttactgga gtgacgatat gatcaccgaa
     5461 gatattgata ttagctggaa gctgcagttg aatcagtgga cgatttttta cgagccacgg
     5521 gcactgtgct ggatattaat gcctgaaacg ttaaaagggc tgtggaaaca gcgcctgcgc
     5581 tgggctcagg gcggtgcaga agtattcctc aaaaatatga caaggttgtg gcgcaaagaa
     5641 aactttcgaa tgtggccgct gttttttgaa tacagcctga cgacaatatg ggccttcacc
     5701 tgcctggtcg gtttcattat ttacgcagtc caacttgccg gtgtaccgtt aaatattgaa
     5761 ttgacacata tcgctgcgac acatactgcc ggaatattat tgtgtacgtt atgtttactg
     5821 caatttattg tcagcctgat gatcgagaat cgctatgagc ataatctgac ttcatcgctt
     5881 ttctggatta tttggttccc ggttattttc tggatgctga gcctggcaac gacattggta
     5941 tcatttacac gagtcatgtt gatgcctaaa aagcaacgcg cccgttgggt aagtcccgat
     6001 cgcgggattc tgagaggtta atatgaacaa tttaattatt acgacccgac aatcaccagt
     6061 acgtttactg gttgattatg ttgccacaac catcttgtgg acattatttg cgttgttcat
     6121 attcttattc gccatggatc tgctgacggg ttattactgg caaagcgagg ccagaagccg
     6181 acttcagttc tattttttgc tggcagtggc gaatgccgtc gtgttaattg tctgggcgct
     6241 gtacaataag ctgcgttttc aaaaacagca gcatcatgca gcctaccaat atacgccgca
     6301 agaatatgca gagagcttag caatacctga tgagctctat cagcaactac aaaaaagcca
     6361 caggatgagc gtacacttca ccagccaggg gcaaataaaa atggttgttt cagaaaaagc
     6421 gctagtcagg gcataaacac ccaaaacaaa gcccggttcg cccgggctct gcaccgataa
     6481 tacacttaac tgtatgcatg cagcgtacgt tggcaaagtg ccgaacgtac gcaatcctct
     6541 tttccgaacc ggacgatccc aaccatttca tcttcttcga aacgttccag cgcgtcactt
     6601 aatccggagc acacgccgcg aggcaaatcg cattgcgtga tatcaccgtt gacgataacc
     6661 gtcacgttct ccccgaggcg ggttaaaaac attttcattt gcgcggcagt cacattctgc
     6721 gcctcgtcaa gaatgacgac tgcattttca aaggtacgtc cacgcatata ggcgaacggc
     6781 gcaatttcca ccttccctat ttccggtcgc aggcagtact gcataaagga agcccctaag
     6841 cgccggacca gcacgtcgta gaccgggcga aaatagggag caaacttttc tgcgatatct
     6901 ccaggtaaga agccaagatc ttcatcggct tgcagaactg gacgggtgac gataatcctg
     6961 tcgacatcct tatgtatcag ggcctctgcc gcttttgctg cgctgatcca ggtttttccg
     7021 cacccggctt cgcccgtggc gaatatcagc tgcttactct caatagcctt cagatagtgc
     7081 aattgcgctt catttcgcgc gaggatgggc gaagtatcgc gactgtcgcg ggccatacca
     7141 atggcttcta cgccgcccat ctgcacaagc gaggtgaccg attcttcttc acgctgctta
     7201 tggctgcgcg aatcccgtct cagcacacgt tttgcctcgc gacgagcttt gatcactgct
     7261 ttttgtcttc ccatggagag caccttgagt tgtttgtatt catcacacgc gccgttggca
     7321 gcgcgattat gcgcacgaac atcagagggt tggcttcctt gtaagccata gtttgctttt
     7381 ggataaaatg ccgaaaaacg gctacgcgca ccgtttacgg cgtcggtaac acatgaaaag
     7441 aaaggatgag gttgaaaatg caaagtgacg agatgactac cggaggagaa aactccgcga
     7501 gtggtggcgc gttgattatc taaaacatgt ccagtacagg acgttaccat ccgcgatctc
     7561 catagtgact gactatcact gccgggaact tccgctgcta cttaataagt acaacagttc
     7621 tcgcatttat tgcaacaata tatttactta tatttaacta taaaacacca tttcagtgac
     7681 attagtttct actggaaaga tgacagagtg atgacagata tgaaaaaagc tgtgtgcttt
     7741 cagcaggatt tgcatcaggc atcagtgcac gatcgccgta tgcggcgtga acgccttatc
     7801 cgcctaccga acccgcaccg attgtaggca taagaagacg cgcaagcatc gcatcaggca
     7861 tctgcgcaag aacgccttat ccgtctaaaa acattaaacc cgcaataacg cgcttccgag
     7921 ataatcgttc gcgtccttca ctcccggcag cgcaaaaaaa taaccgccgc cgataggttt
     7981 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     8041 cagatcgtgt tggtaacaga caaacagtag ccccatatcc agttgcccgg agttggtgac
     8101 gcccagtgaa taactgtagc cacgacgcag catcaggctg gactcactct ccgccgtgcg
     8161 gggattcgcc agccggatat ggctgtccag cgcaatcccc ttcccttccg ggtcgctggc
     8221 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     8281 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     8341 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     8401 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tagcagtgcc
     8461 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     8521 ggaaataaac ccttcccgct tccagcgcac gctgagcaaa tccggcgtat gtttgatgat
     8581 atcgcgcagt gcatggatca ccgtgtcctg ggtgttggcg caaatttgta gcaacacatc
     8641 accatgacat aacgccgcat ccagcgagtc gttgggaaaa cgcgtcatct tctgcaactt
     8701 tttcggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     8761 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     8821 caggcgcgga tttggcgttt ctggtgccgc tccgccctga gtcagaaaag caaaacgctg
     8881 agtcaacaag cgaaacaacc gttcaagatc ggctttatcg ctggcaagca catcaaacgc
     8941 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     9001 cggctgtttc tcattgcgtg caaccggtga aagcgtaccc ggcgcacttt gcgttttttg
     9061 tgcatgagcg accggacaac ttcccgccag cgccagcgca ccaatgcctt tcagtaaacg
     9121 tcggcgtgac ggttcattca cgccgttttt atcttcatac tgcataatgc ttaatccaat
     9181 cccagcacac cgcgaagttg cgccagatct tccgccagcg cagtaatcgg tcctttcagt
     9241 gcattccggt cggcatcggt caatttgtcg taggtttcaa aaccgtcttt agtacggtat
     9301 ttcgccagaa tggtatcgac ctttttaaag ttggcatcga cttttgccaa cagttccggg
     9361 ttagcttttt gcagttgtgg acgcagcaga tcgacaattt tctgcgagcc ttcaacgtta
     9421 gcctggaaat cccacagatc ggtgtggctg tagcgatctt cttcaccgct gattttgctg
     9481 gctgccactt cctcaatcag tccggctgca ccgccgacca cttttgaagg tgggaaagcc
     9541 agttcactga tgcgtttttg caaatcgacc acatcggtat aaagctggtc agcgtactga
     9601 tccatgcctt tggtggtgtt gtcgccaaac aatgcttttt ccagacggtg gaaaccggtg
     9661 aattttggat cagcggcttt ttgctcgtaa tcatcttcac gggcgtcaat gctgccatcc
     9721 agatcggaga acagttcagc aatcggttca atgcgctcat agtgctggcg cgtcggcgca
     9781 tacagtgctt tcgctttttc gatatcgcct gctttaatcg cgtcggtaaa ggctttggtg
     9841 tcggtcacca gctgtgtggt ttccgccatg acatacgctt tatatgcagt aattgcacca
     9901 ccaagactta acagcgcatc actttgcgcc gcatccgccg ttgcctcacc tttgacgatc
     9961 aacttccctt tcgggttagt cagcagaccg caggtcatat cgtattcgcc aggctgtaaa
    10021 ttcgccgtca ttttctggct aaagccaggg gcgatatttt cccgctcttc caccaccatc
    10081 acgcctttga ggatctccca ttccagcgcc ttctggctgt ggttctgaat aatgaactgt
    10141 gttttcccgg cgttaaccgt aatggtcatc ggttcgcact gcttatccgt cacggtcact
    10201 ttgacctgcg gcacatcagc ggcgttagcc ataaaagcag aagaaaacag cgcagccacg
    10261 ctcaactgca atgcgttacg gcggaagtta atggtcatga cgagactatc cctttaaagt
    10321 atgttgtaac taagcaagag tttgcgtcgt atttgttacg cggagcgaga cgctgtcgcc
    10381 cctgcgcgtg gtggcagagc aaatgccacc agcgccggga tgagataaat aaaccagacg
    10441 gcgacttcgc tgacgctcgg cgcttcctga tagccaaaaa tcccttccat cagcgtgcca
    10501 aacagcgagt gagttgagag caccgcactc atatcgaagg cgatttcctg aaagtggttc
    10561 cacaatccgg cttcatgaaa tgcgcgaatg gcaccagctg ccagccctgc ggcgacgaag
    10621 agaataaaca ggctggtcca tttaaaaaat gcaccaagat tgaggcgaat accgccccag
    10681 tagagcagga agcctagcac cacggcagta gcaagaccga gcattgcacc cagcggcggc
    10741 cagatcccga catcttgttg aaatgccgcc agcaggaaaa agaccgactc cagcccttcc
    10801 cttgcaacgg caaaaaagac catcatcacc agcgcccagc catgatgatt tccacgctgc
    10861 aatgcgctat cgacagcctg ctccagttgt actttgacgt tgcgcgacac tttgcgcatc
    10921 cagaaaacca tccaggtaag gatcaccacg gcgatcaccg ccacgatacc ttcaaacagt
    10981 tcctgctctt tttgcggaaa ttcgccggtg gtttcgttaa tgaagatccc caagcccagg
    11041 cacaacgcag cggcaagcaa cacgccaatc cacatcacac caatccatcg gcctcgctgg
    11101 gtacgcttaa gatagctggc aatcaaactg acaatcagcg cggcttcaag tccttcgcgc
    11161 aacataatga gaaacggaac aaacatgcca cccaccctta aacgtttttc tgagtcaatc
    11221 tatgcaaaga a
//
LOCUS       DABSYT020000130        10999 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.130, whole genome shotgun sequence.
ACCESSION   DABSYT020000130 DABSYT020000000
VERSION     DABSYT020000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.130"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..411
                     /locus_tag="ID725_RS21200"
                     /old_locus_tag="pgaptmp_004240"
     CDS             <1..411
                     /locus_tag="ID725_RS21200"
                     /old_locus_tag="pgaptmp_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGV7852715.1"
                     /translation="RQTVAARMADVPLAPGSNEKKKLYLLTDLITSLLEKPPSSEDED
                     MDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSFSVVVKAIVQVLETWPDRLERDR
                     GWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            408..1412
                     /locus_tag="ID725_RS21205"
                     /old_locus_tag="pgaptmp_004241"
                     /pseudo
     CDS             408..1412
                     /locus_tag="ID725_RS21205"
                     /old_locus_tag="pgaptmp_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1577..2815
                     /locus_tag="ID725_RS21210"
                     /old_locus_tag="pgaptmp_004242"
     CDS             1577..2815
                     /locus_tag="ID725_RS21210"
                     /old_locus_tag="pgaptmp_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7852716.1"
                     /translation="MSVKPLTVTEVKGMKPREKDYAVYDGFGLLLNVSKAGGKVWRFR
                     YSHPITKKRQTYTIGRFPEFSLAEAREVRDELRRMIARGVDPVTEKKNRKIEMSLKNL
                     QTFEAVANAWFAFKKGSELRKPTLYNIEYEVYKYLVPFFGKYSIEKITAPVAINALDA
                     VSDKNALQKKLISRLNEIMNYAVNCGALKANPLLKIKTAFTGKKNKSLAALPVERLPE
                     FLSWWDSVPHTYQIAHNALLFQILTMVRPGEAIKAEWSEIDFDSGLWIIPAHKMKCHR
                     EHVVPLSSQAISILRTMQEIKRGRYVFFSSRTKDAPMGRNTIKTPIAASKFKGIVTLH
                     GFRSMWSTLLNEEGFNPDVIEAALAHKSGDKIRDIYNRTIYLEQRKIMMQWVGDFFDD
                     ARKGVINRSGGMKGLRVVNG"
     gene            2829..3032
                     /locus_tag="ID725_RS21215"
                     /old_locus_tag="pgaptmp_004243"
     CDS             2829..3032
                     /locus_tag="ID725_RS21215"
                     /old_locus_tag="pgaptmp_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7852717.1"
                     /translation="MNTNEDILFTKDVMKILRYGAMSAFINFWKDENNGFPQPFRIGR
                     RHTWHRRDVEAWLDKQREQANPH"
     gene            3092..3673
                     /locus_tag="ID725_RS21220"
                     /old_locus_tag="pgaptmp_004244"
     CDS             3092..3673
                     /locus_tag="ID725_RS21220"
                     /old_locus_tag="pgaptmp_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HGV7852718.1"
                     /translation="MNKNIAVTGKGDARHVKKFCDFRDLVVLRFDSVNVRVVYLNGDP
                     WFVAKDVCVALEISNSRDALKALDADEKKTVALSYGIRGNPNHSLISESGFYKLIARS
                     RKAVTPGTFAHRFSNWVFRNVIPGIRKTGAYGIPWGALQDFSRRKEQYQLSASQKGRE
                     LQACKRKKRELEEEEKRLIREYQPEFYFGNRIQ"
     gene            complement(3794..3973)
                     /locus_tag="ID725_RS21225"
                     /old_locus_tag="pgaptmp_004245"
     CDS             complement(3794..3973)
                     /locus_tag="ID725_RS21225"
                     /old_locus_tag="pgaptmp_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGV7852719.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKEAE"
     gene            4170..4373
                     /locus_tag="ID725_RS21230"
                     /old_locus_tag="pgaptmp_004246"
     CDS             4170..4373
                     /locus_tag="ID725_RS21230"
                     /old_locus_tag="pgaptmp_004246"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGV7852720.1"
                     /translation="MRNHTTHPQGRDLHNLNKFTWRFIALSTAQPRVITIEATSEQEA
                     RQQSPVGCVMVFAARIRQGVEHV"
     gene            4366..4590
                     /locus_tag="ID725_RS21235"
                     /old_locus_tag="pgaptmp_004247"
     CDS             4366..4590
                     /locus_tag="ID725_RS21235"
                     /old_locus_tag="pgaptmp_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852721.1"
                     /translation="MCNATWPDAAVDAIKTLMDSLIEISAIAGVAHKHAARESECIFH
                     YLAFVQLKADQALDKAGKIIMADVQEVHHA"
     gene            4583..4942
                     /locus_tag="ID725_RS21240"
                     /old_locus_tag="pgaptmp_004248"
     CDS             4583..4942
                     /locus_tag="ID725_RS21240"
                     /old_locus_tag="pgaptmp_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852722.1"
                     /translation="MHNLSISDLNSIQFDEIFSGQLLVNVENGRVVSNYHLPDGAIAG
                     SVEALLELAERARLIKPSTCHHDDDLHFTGRMVSHYENGVEVSRERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGGG"
     gene            4945..5178
                     /locus_tag="ID725_RS21245"
                     /old_locus_tag="pgaptmp_004249"
     CDS             4945..5178
                     /locus_tag="ID725_RS21245"
                     /old_locus_tag="pgaptmp_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852723.1"
                     /translation="MRDDRFNSLKREFDGAPEDAAGALLSVADMMKAAYFLINTSGYK
                     SEGEMILSIASDYAEYVAETRYRRKFTEDVSHA"
     gene            5171..5370
                     /locus_tag="ID725_RS21250"
                     /old_locus_tag="pgaptmp_004250"
                     /pseudo
     CDS             5171..5370
                     /locus_tag="ID725_RS21250"
                     /old_locus_tag="pgaptmp_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5375..5617
                     /locus_tag="ID725_RS21255"
                     /old_locus_tag="pgaptmp_004251"
     CDS             5375..5617
                     /locus_tag="ID725_RS21255"
                     /old_locus_tag="pgaptmp_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGV7852724.1"
                     /translation="MKKKNSGFTASGLSRPEIRLGDIYRDTRRGGRVVIRHVTPGNIT
                     YRRDFTLVDHYKAVNQKRAASYIRKIREMLVAGGKK"
     gene            5614..7428
                     /locus_tag="ID725_RS21260"
                     /old_locus_tag="pgaptmp_004252"
     CDS             5614..7428
                     /locus_tag="ID725_RS21260"
                     /old_locus_tag="pgaptmp_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HGV7852725.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEHDARMA
                     GDNEPPVVLADEQLKEIGNLQIVPDGRTSARIFRAGQLDPVMVKAIGQKLAAASIQNA
                     NYYPDGMHSQKCENWREYLARERKNIDDGLIIRFPAKKKTEDSATPLALNQMGASQRG
                     EVLLAHYGGELAINADSDTVHHYNGVVWEPVQDKELQRTMAQIFIDAEISYSQNAIKS
                     AVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEGE
                     TLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFLEVTGPGGSGKSVM
                     AEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGGD
                     KVAIDPKHKAPYSTRIQAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDPM
                     LAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYLMS
                     SVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYGR
                     EYMKRKTKHGLRSNVILTEDSEDWMPSCASVTNDDGKN"
     gene            7716..7961
                     /locus_tag="ID725_RS21265"
                     /old_locus_tag="pgaptmp_004253"
     CDS             7716..7961
                     /locus_tag="ID725_RS21265"
                     /old_locus_tag="pgaptmp_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852726.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELV
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            7958..8380
                     /locus_tag="ID725_RS21270"
                     /old_locus_tag="pgaptmp_004254"
     CDS             7958..8380
                     /locus_tag="ID725_RS21270"
                     /old_locus_tag="pgaptmp_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGV7852727.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATMWLSVLAFGRQADALAKHRKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            9039..10928
                     /locus_tag="ID725_RS21275"
                     /old_locus_tag="pgaptmp_004255"
     CDS             9039..10928
                     /locus_tag="ID725_RS21275"
                     /old_locus_tag="pgaptmp_004255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGV7852728.1"
                     /translation="MKTWFSIKAMADVVHVRIYDEIGGYGVKASALTDEINACGNVSE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVMPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITAMMEAETWMDG
                     NECKANGFADEVIAAITAMARIESKRIGDFSNMPEKIKSMISQKTTSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYGTRSQIVNA
                     AFTHSSSDFTDILAGGAEKSVLAGWEHSGETFRQWTKKGSLSNFREARRVGMNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLDQLSTVPMAMGRAASR
                     TVGNLVNLVLTGNVKLSDGIALFDKKHSNLIEAGLTTPGLSAARHLMRTQKDKNGEVL
                     NIAPKFLLVPAALEDRALQMINSTAPFGADKNSGIFNPYHKLLDIIVDPRLDDISEKQ
                     WYMLSAQGTDTIEVAYLDGSDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVKSG
                     GTDSL"
BASE COUNT         3077 a         2363 c         2946 g         2613 t
ORIGIN      
        1 cggcagactg ttgccgccag aatggcggat gttcctcttg caccaggcag taatgaaaag
       61 aaaaaactgt atctcctgac ggatttgata acttcgttgc tggaaaaacc accatcttcc
      121 gaagatgagg atatggatcc tcacgctcgt aaggcatggt atcagtccga gcgcgagcgt
      181 cttaaatttc agcatgaaac tgttcagctt gtgccagtca gtgatgtcag gcggtccttt
      241 tctgtcgtgg tgaaagcgat agttcaggta ctggaaacct ggcctgaccg gctggagagg
      301 gacagggggt ggactgcatc acaactgaat gaagtacaga ttgtggttga tgagatccgc
      361 gacacactgg aaaaggcagt cattgactgt tgtgatgagg ccgatatgtg aatcaggtga
      421 acgagagcca tagccgcgca tccgatatct ggcgcgaagt ggcctcgctg tttcgcccac
      481 ccagccggtt accagtagcg gaagccatca ggcgttatat gcgggttcca cggggagcca
      541 atacttccgg tccgtgggag tcatcgctga cgccctatat gattgacccc attaatacat
      601 tatcagcccg tgaatatgac gcggtggtgt ttgtgggacc tgcgcgaacc gggaaaaccg
      661 aagggctgat tgatggctgg attgtgtacg gcatcatctg tgatccggcg gatatgctgg
      721 tggtgcagat gactgagacg aaggcgcgtg agcattccag aacgcgtctt tccaggacgt
      781 ttcgccacag tccggaggtc agcaagcgcc tcagtccttc ccgtaatgac aacaacgtcc
      841 acgataaaat gtttcttgac ggctccttcc tgaagattgg ctggccgtcg atcaccgtct
      901 tttcctcttc ggattaccgt cgtgtggcgc tgacggatta tgaccgtttc cctgaaaatg
      961 tggacgggga aggggatgcc ttcacgctgg cctcaaagcg taccaccacc tttatgtcct
     1021 cggggatgac cctggtcgag agttcaccgg ggcgggatat caccgatacc aaatggcgtt
     1081 gtggtggcgc acatgaggca ccgccaacaa cggggatcct gtcactgtat aaccggggag
     1141 accgccgccg gtggtactgg ccgtgtccgc actgcgggga atattttcag ccggtgatgg
     1201 ataacatgac cggataccgg aataacccgg attttgtggc tgccggtcag gctgcccgtc
     1261 tgatgtgtcc gcattgtcgc gggctgattg cccctgagca gaaacgcgaa ctgaataacc
     1321 aggggatctg gcttcgtgaa ggtgaacggg cggcggcgga cggcagtatc accggaacgc
     1381 cacgaaactc ccggattgcg tcattttgga tgtagggtac atttcttacc tgtttgtatg
     1441 ttctggtgtc gtttcatagt cttttcaatg agttgtgatt ttatgagttt cctctctttg
     1501 cttgataatg agttagttta tcgcttgtta ttggcttgaa tggactacat gacggactaa
     1561 aaaatgaggg cgatagatgt cggtaaagcc attaaccgtg actgaagtta aggggatgaa
     1621 accacgtgaa aaggactatg ccgtttatga tgggttcggt ttattgctga atgtgagtaa
     1681 agccggtggg aaagtgtggc gtttccgtta tagccatccg ataacgaaga aacggcagac
     1741 atacacgata ggacgttttc ctgaattctc actcgcggaa gcacgggaag tacgtgatga
     1801 acttcggcga atgattgcac gtggagttga tccagtgacg gagaagaaaa atcgtaaaat
     1861 tgagatgtca ctaaaaaatc tacagacatt tgaagctgtt gctaatgcat ggttcgcttt
     1921 taaaaaggga tctgaattgc ggaaacctac gctgtataat atcgaatatg aagtatacaa
     1981 atatcttgtt cctttctttg gtaagtacag tatagaaaaa attacagcac cagtagctat
     2041 taatgctctg gatgccgtat ccgataagaa tgcgttgcaa aaaaaattaa tatcaagatt
     2101 aaatgaaatt atgaattatg ctgtaaattg tggagcattg aaagcaaatc cattacttaa
     2161 gataaagact gcattcacag gaaagaaaaa taaatcatta gcagcactac ctgttgaaag
     2221 attgcctgaa tttctgagct ggtgggatag tgtgcctcat acctatcaaa tagctcataa
     2281 tgcactttta ttccagatat tgacaatggt caggccaggt gaggcgatta aagcagagtg
     2341 gtcagagatt gattttgatt ctggcttgtg gattatcccc gcgcataaaa tgaaatgcca
     2401 tcgtgaacat gttgttccct tgtcatcaca ggctattagt atcctcagaa caatgcagga
     2461 aataaaaaga gggcgttatg tgtttttttc ctccagaaca aaagatgcgc ctatggggag
     2521 gaatactatc aagaccccaa ttgctgccag taagttcaaa gggattgtaa cgttacatgg
     2581 ttttcgttca atgtggagta cgcttttaaa tgaggaggga tttaaccccg atgtaatcga
     2641 ggctgcattg gcgcataaaa gtggtgataa aataagagat atttataata gaactattta
     2701 tctagaacag cgtaagatca tgatgcaatg ggtcggtgat ttttttgatg atgcgagaaa
     2761 aggggtaatt aatagatccg gtggtatgaa aggtttaaga gtagtaaatg gttgaggagg
     2821 ttcagcaaat gaataccaat gaagatattt tatttactaa agacgtaatg aaaatcttgc
     2881 gatatggagc aatgagtgca ttcatcaatt tctggaaaga tgagaataat ggttttcctc
     2941 agccgttcag aattggacga cgacatacct ggcaccgtag agatgtagaa gcatggttag
     3001 ataaacaacg agaacaggcc aatccccact aataatatct ttcatacccc gcgtgcaatg
     3061 cggggttttt tgtatgtgag gtaaaaaaga aatgaataaa aatattgccg tgacgggcaa
     3121 gggtgacgca cgtcatgtaa aaaaattctg tgattttcgt gatctggtcg ttctgcgctt
     3181 tgatagtgtg aacgttcgcg tggtgtatct gaacggcgat ccgtggtttg ttgcaaagga
     3241 tgtttgtgtt gctctggaaa taagtaactc acgcgatgct ctaaaagcac tagatgcgga
     3301 tgaaaagaaa accgtcgctt taagctacgg tattcgagga aatccaaatc atagcctcat
     3361 ttctgaatcc ggtttttaca agctgatcgc cagaagtcgg aaagctgtta ctcctggcac
     3421 gttcgcccat cgtttcagta actgggtatt caggaatgtg ataccaggaa tcagaaaaac
     3481 gggggcttat ggtatcccgt ggggcgcatt acaggacttt tctcgccgta aagagcaata
     3541 tcagttaagt gccagtcaga aggggaggga gctacaggca tgtaagcgca aaaagcgtga
     3601 gctggaggaa gaagaaaaaa ggctgatacg tgaatatcag cctgagtttt actttggcaa
     3661 ccgcattcag tgacaaaaca aaggcgaccc ggatcagagg tcgccaatgg gaaaacacta
     3721 aacataagcc caaacatcat agcgatttgt tggctggtgg gcaatgtgat cagtgtgctg
     3781 gcttgctggt ggactattcc gcttctttgg ccttgcggcg ctggcggcgt ttgatctcgc
     3841 ctcgcatggc tgtaacgata aattgcgcag tgctctcgcc tgtttctttt acttgctcca
     3901 tagcctcaac aacttcatgc ggggcgcgag cctgtaattt ctgtgatttg ttattgatat
     3961 ggtctctttc catttctggt tccctttgtg attactggaa tccagtatac acgaaaaaga
     4021 ccaaaacaaa acgcttgaag tggattccac ttagtgatta tactggaatc cagttgagtg
     4081 tttatcactc aatgcaaacg aggctgaaaa ggattgccgt cctttcgcag cctctgacca
     4141 ccaacgatag caacagtatc gaggtagcta tgagaaatca taccacacac ccgcaagggc
     4201 gggacttgca caacctgaat aaattcacct ggcgttttat cgccctgagc acggcacaac
     4261 cgcgcgtgat taccattgag gccaccagcg aacaggaagc acgccagcaa tccccagttg
     4321 gctgcgtgat ggtattcgcc gcccgtattc gtcagggggt ggaacatgtg taatgcaaca
     4381 tggcctgatg cagcggtaga cgctatcaaa acgctgatgg attcacttat tgagatttct
     4441 gctatcgctg gtgtggcgca taaacacgca gccagagaat cagaatgcat cttccattat
     4501 ttagcatttg tgcagctaaa agccgatcag gcactggata aggccggaaa aattatcatg
     4561 gctgatgtgc aggaggtgca ccatgcataa cctgtcaatt tctgacctta acagcattca
     4621 gtttgacgag atattttccg ggcagctact ggtcaacgtg gagaatggac gcgtggtaag
     4681 taattatcac ctgccggatg gtgcaattgc cggaagcgtt gaagcattgc tggaactggc
     4741 ggaacgtgcg cgactgatta agccgtcaac gtgccatcac gatgatgatc tgcattttac
     4801 cggacgcatg gtgagtcact acgaaaacgg cgttgaagta tcccgcgaac gtctgcgtga
     4861 tgattgctgt ttcggcacac tgccggaatt tatcgaattg ctgaccagtt gcggttatca
     4921 ggtcattcag gggggggggt aaacatgcgt gatgatcgtt ttaattccct gaaacgggaa
     4981 tttgatggcg caccggaaga tgcagcgggc gcattgttga gcgttgctga catgatgaaa
     5041 gctgcatatt ttcttatcaa taccagtggc tacaagtcag agggtgaaat gattcttagt
     5101 attgcgtcgg actatgcgga atatgtggca gagacgcgtt acagaagaaa attcacggag
     5161 gatgtaagcc atgcataatc atgaagcgca tgtacccgta gtgcttaatg tgccagatga
     5221 tttcaccgga cgcgtactgg tttacctgga taaagggaaa gtgaaatcac aatgccgact
     5281 gaaaagtaat gagattgttg gttctcctga attttttctg aactctgtat tcgtgcggaa
     5341 ataaaaccgg aactgctgac aggaaaataa aactatgaaa aagaaaaatt ctggctttac
     5401 tgccagcggt ctctctcggc ctgaaatcag actcggagat atttaccgcg acaccagacg
     5461 tgggggacga gtggttattc gtcacgttac gccaggcaat atcacctacc gccgtgattt
     5521 tacgctggtg gaccattata aagccgtaaa tcaaaaacgt gcagcaagct atatccgcaa
     5581 aatccgtgaa atgctggttg caggggggaa aaaatgaaac tggcaccgaa cgtaaaaaaa
     5641 cagccacgcg gaataaaaca caaagacaca gaggtaatta ttttcgcggg tagtgatgcc
     5701 tgggcacacg cgaaacaatg gcaggaacac gatgcgcgta tggctggaga taatgagcca
     5761 cctgttgtgc tggctgatga acaactgaaa gagatcggaa atctgcaaat agtgcctgat
     5821 gggcgcacat ccgcgcgcat attcagagcc ggacagttag atcctgtaat ggttaaggca
     5881 attggtcaga agctggcggc ggcaagtata cagaatgcga attattaccc tgatggcatg
     5941 cacagccaga agtgtgagaa ctggcgtgaa taccttgccc gtgagcgtaa aaacattgat
     6001 gacggactga ttatccggtt tccggcaaag aaaaaaacag aagacagcgc gacaccactg
     6061 gcacttaacc agatgggagc aagccagcgc ggcgaagttc tcctggcaca ttatggcggt
     6121 gaactggcaa tcaatgcgga ttctgacacc gttcaccact acaacggtgt tgtatgggag
     6181 ccggtacagg ataaagagtt acagcggact atggcgcaga tttttattga tgcggaaatc
     6241 agctattcgc agaacgccat caaatcggcg gtcgatacca tgaagttaag tttgcccgta
     6301 atggggaata cagcccgtaa cctgattgga ttcagtaacg gggtatttga taccagaact
     6361 ggtaattttc gggagcataa caaaaacgac tggttgttaa ttgccagtga attacctttc
     6421 agcccaccag cagaggggga aacgctggca acacatgcgc cgaatttctg gaagtggttg
     6481 cgccgttcgg tggctgagaa tgaccgcaag gcagatcgcg tactggcggc gttattcatg
     6541 gtgctggcga atcggtacga ctggcagtta tttcttgagg taacggggcc gggtggaagc
     6601 ggtaaaagcg taatggcgga gatttgcact atgctggcgg gtaaggccaa cacggtatcg
     6661 gcaagcatga aggcgctgga agatgccagg gaacgtgcgt tagtggttgg cttttcgctg
     6721 attatcatgc cggatatgac ccgttacgct ggtgatggtg cagggattaa ggccattaca
     6781 ggcggtgata aggtggcaat cgacccgaaa cataaagccc cctactcaac acgcattcag
     6841 gcggtcgtgc tggcggtgaa caataacgcc atgtcattca gtgaccgcag cggtgggata
     6901 tcacgtcgtc gggtaatatt caatttttcg gaagttgtac cggagaacga acgtgaccct
     6961 atgctggctg aaaaaataga aggagagctg gcggtagtga ttcgccacct gcttacacgc
     7021 ttttctgacc aggacgaagc taaacgcctg ctatatgagc agcagaaatc agaagaagcc
     7081 ctggtgataa aacgcgaggg cgattcgctg gtggacttct gcggctatct catgtcgtcg
     7141 gtaatgtgcg atggcctgtt agtgggtaat gctgaaatta ttccgttcag cccgcgcagg
     7201 tatctctatc acgcctatct ggcgtatatg agggcacacg gattcggtaa gcccgtaaca
     7261 ctgacacgct tcggtaagga tatgccaggg gcaatggcgg aatacggcag ggagtatatg
     7321 aaacggaaaa cgaagcatgg cttgcgttca aacgtgatac tgacagagga ttcagaagac
     7381 tggatgccct catgtgcatc ggtcacaaat gacgatggca aaaattaaac ttatggaata
     7441 actgttcatc actgttcacc ctgtcataaa tatcttttat atcagtatat tatatggtga
     7501 acagttattt atgaactgtt caccaaacta ttcactgttc acctttttga ttgtttattg
     7561 agcttcaagg gtgaacagtg gtgaacagtt ggtgaatagt ttttgtgaaa ctgttcaccc
     7621 cttaacatta tgaattaaaa gataaaatat caaaaggtga acaggtgaag ggttgaaacg
     7681 caaaaatttt aatttactgc tgtgagataa agcctatgac agcgaagcac acaaaaaaat
     7741 cacaatcgca cgcccttgat ttgacggaac actggttaag ggtgtcgata aaaatcatcg
     7801 accgcaacgc cggggaagga tatgcgaaag cacatcccga actggttagc gcattcatga
     7861 caacggcagc tgcaaacttt gccacgctga cagaacggga gattgccgaa gcggaacagg
     7921 taacaaccat caacgttaaa accggagagg tggaatcatg acagcacaga tagcggctta
     7981 cgggcggctg gtggctgacc cacagttaaa gaccaccagc aagggcacac aaatgacgat
     8041 ggcgagtatg gctgtccccc tgccgtgcag ccaggcagat gacggaacgg ctacgatgtg
     8101 gttatccgtc ctggcgtttg gcagacaggc cgacgcactg gcaaaacacc gcaaaggcga
     8161 gctggtgagc gtggcgggta acatgcaggt aagccagtgg acaggccaga acggcgaaac
     8221 gcggcagggc tggcaggtta tcgcagacag cgtaatcagt gcgagaacgg cgcgaccggg
     8281 cggcaaaaaa ggccagcagg ggcaggctac tgacgcactg aacagggcaa aacaacaggc
     8341 ggggaatgat gatccgtacg gggataacat accgttttaa gcaacgagtg acagaagccg
     8401 gagaaatccg gcttttttgt aggtactcct ggtgggggtg gcctgtccac ggggcggagg
     8461 ggcgcggaaa aaggcgcatt ttttgatttt tatggcacca tcaccaccac tataagctat
     8521 tgatatgttg agaaataaaa atttttagtg tcgaatcagg ttgttttttg ttcatcaccg
     8581 gaacgttccc gaaaacattt acaaaaaaca ggcgcaaaaa aaagcgcccc cgattgctgt
     8641 taccggaggc gcttttacac gacaaaggag tttttatcgc caggatgacg agtcttaata
     8701 ctgcttcaat ggcaaaaatg cgtcaataac tttgcctttc tgagaataat cagaaaaaac
     8761 ataatctgat tttcaggtag aaaatgattt atctattact tttatcgatc aataatgatg
     8821 cccgttaatc aaaacggagg cggatttatg ccagagaaca acaccagaaa gccggataaa
     8881 agtgccacgg tacacataga cgccggaact atggagaaga tcgaacgcta tcagcagttc
     8941 atcaaagata atcacccggg tatgccagtg cccacgaaag gacaaatcac acgcagcgcg
     9001 gttgaatact ggtacagggc aacgttagga gcctggctat gaaaacatgg ttttccatta
     9061 aggctatggc agatgttgta catgtgcgca tttatgacga gatcggcggg tacggtgtaa
     9121 aggcatcggc acttactgac gagatcaacg cgtgcggtaa tgtgtctgaa atccatcttc
     9181 gcatccattc acctggtggc gacatctttg aagggctggc tatctataac gccctgaaaa
     9241 atcatccggc aaagaaaatt gtacacattg aaggcatggc ggcttctatg gcctcgttta
     9301 ttgccatgtg tggcgatcac atcgttatgc ctgaaaacgc gatgatgatg atacatgccc
     9361 cccgtggtgt tactgccgga gtgtcgggcg acgttcgccg ctttgctgac ctgatggaca
     9421 agctgggcga cacgatggcg gaaacctacg ccggaagaac gggcaggagc aaacaggaaa
     9481 tcaccgccat gatggaggcg gaaacctgga tggatggcaa tgagtgtaag gctaacggct
     9541 tcgcagatga ggttatagcc gcgattacag caatggcccg aattgaatca aaacgaatcg
     9601 gagatttttc aaatatgccg gaaaaaatta aaagcatgat cagccagaaa actaccagtg
     9661 gcgaacagga acgacttaac ggcatccgtg aattgtttgg cacgttcaac ggaagatata
     9721 acgacctggc tataagttgt cttgctgatt cagaatgtag cgttgagaat gcacgcgaac
     9781 gccttttact cgctatgggt aaagaatcaa cgccaacaaa caaaaccacc cccgcaaatc
     9841 tttactacgc gtacacggat aacggcaaca taaccggcga tgcaatgcgc caggggctta
     9901 atgcgcgtct tggtcacgaa cgggccgaac gcggtaatcc ttacgccatg atgagccttt
     9961 tcgatatggc acaggcatca ttaacccatc gtggtataag cacgggcagc tacggcacac
    10021 gctcgcagat agtaaacgcg gcattcaccc acagcagcag cgattttacc gatattcttg
    10081 ctggtggcgc tgaaaaatca gtgcttgcag gctgggagca cagcggcgaa acattccgcc
    10141 agtggacgaa aaaaggttcc ctttcaaact tccgggaagc ccgccgcgtt ggtatgaatg
    10201 gcttctcaac gttaaacaaa gtgccggaag gggcagaata taaatacatc accaccagcg
    10261 atcgcggtga acccatcgcg ctggctactt acgggaatat tttcagcatt acccgccagg
    10321 cgataatcaa tgatgacctt gatcagttat caacggtgcc aatggctatg ggccgtgctg
    10381 catcaagaac ggtgggaaat ctggttaatc tggtgcttac aggcaacgta aaactttctg
    10441 acggaatagc gctgtttgac aaaaaacaca gcaacctgat tgaagcagga ctgacaacac
    10501 cgggacttag tgcagcacgt cacctgatgc gcacacagaa ggacaaaaat ggcgaagtgc
    10561 tgaatattgc gcctaaattc cttttagttc cggcagcact ggaagatcgc gcgttgcaga
    10621 tgattaactc aaccgcacct ttcggggctg ataaaaacag cgggatcttt aacccgtatc
    10681 acaagctact tgatatcatc gtcgatcccc gccttgatga tatcagcgaa aaacaatggt
    10741 acatgctttc cgcacaggga acggacacaa tcgaggtggc ttatcttgat ggcagtgacg
    10801 agccttacct tgaacagcag gaaggtttta tcgttgacgg cgtggcctgg aaagtccgta
    10861 ttgatgcagg tgtggcagct ctggattatc gcggtatggt caaatcaggc gggacagatt
    10921 cactatgaca acaaggcggc accagccgcc ttttttgcgg gtcctcctgg tggggtgggc
    10981 ctgaacacgg ggcgggcgg
//
LOCUS       DABSYT020000131        10912 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.131, whole genome shotgun sequence.
ACCESSION   DABSYT020000131 DABSYT020000000
VERSION     DABSYT020000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.131"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            132..782
                     /gene="csgD"
                     /locus_tag="ID725_RS21280"
                     /old_locus_tag="pgaptmp_004256"
     CDS             132..782
                     /gene="csgD"
                     /locus_tag="ID725_RS21280"
                     /old_locus_tag="pgaptmp_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGV7852729.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            787..1176
                     /gene="csgE"
                     /locus_tag="ID725_RS21285"
                     /old_locus_tag="pgaptmp_004257"
     CDS             787..1176
                     /gene="csgE"
                     /locus_tag="ID725_RS21285"
                     /old_locus_tag="pgaptmp_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGV7852730.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1201..1617
                     /gene="csgF"
                     /locus_tag="ID725_RS21290"
                     /old_locus_tag="pgaptmp_004258"
     CDS             1201..1617
                     /gene="csgF"
                     /locus_tag="ID725_RS21290"
                     /old_locus_tag="pgaptmp_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGV7852731.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1644..2477
                     /gene="csgG"
                     /locus_tag="ID725_RS21295"
                     /old_locus_tag="pgaptmp_004259"
     CDS             1644..2477
                     /gene="csgG"
                     /locus_tag="ID725_RS21295"
                     /old_locus_tag="pgaptmp_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGV7852732.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVTQSATAMLVTALKDSRWFIPLKRQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2541..3032)
                     /gene="ycdZ"
                     /locus_tag="ID725_RS21300"
                     /old_locus_tag="pgaptmp_004260"
     CDS             complement(2541..3032)
                     /gene="ycdZ"
                     /locus_tag="ID725_RS21300"
                     /old_locus_tag="pgaptmp_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGV7852733.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3134..3688)
                     /gene="ycdY"
                     /locus_tag="ID725_RS21305"
                     /old_locus_tag="pgaptmp_004261"
     CDS             complement(3134..3688)
                     /gene="ycdY"
                     /locus_tag="ID725_RS21305"
                     /old_locus_tag="pgaptmp_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGV7852734.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3712..4449)
                     /gene="ycdX"
                     /locus_tag="ID725_RS21310"
                     /old_locus_tag="pgaptmp_004262"
     CDS             complement(3712..4449)
                     /gene="ycdX"
                     /locus_tag="ID725_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGV7852735.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4504..5442)
                     /gene="ghrA"
                     /locus_tag="ID725_RS21315"
                     /old_locus_tag="pgaptmp_004263"
     CDS             complement(4504..5442)
                     /gene="ghrA"
                     /locus_tag="ID725_RS21315"
                     /old_locus_tag="pgaptmp_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGV7852736.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5676..5763
                     /locus_tag="ID725_RS21320"
                     /old_locus_tag="pgaptmp_004264"
     tRNA            5676..5763
                     /locus_tag="ID725_RS21320"
                     /old_locus_tag="pgaptmp_004264"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5710..5712,aa:Ser,seq:gga)
     gene            complement(5913..6755)
                     /locus_tag="ID725_RS21325"
                     /old_locus_tag="pgaptmp_004265"
     CDS             complement(5913..6755)
                     /locus_tag="ID725_RS21325"
                     /old_locus_tag="pgaptmp_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGV7852737.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6840..7037)
                     /locus_tag="ID725_RS21330"
                     /old_locus_tag="pgaptmp_004266"
     CDS             complement(6840..7037)
                     /locus_tag="ID725_RS21330"
                     /old_locus_tag="pgaptmp_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGV7852738.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7049..7540)
                     /locus_tag="ID725_RS21335"
                     /old_locus_tag="pgaptmp_004267"
     CDS             complement(7049..7540)
                     /locus_tag="ID725_RS21335"
                     /old_locus_tag="pgaptmp_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGV7852739.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7537..7911)
                     /locus_tag="ID725_RS21340"
                     /old_locus_tag="pgaptmp_004268"
     CDS             complement(7537..7911)
                     /locus_tag="ID725_RS21340"
                     /old_locus_tag="pgaptmp_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGV7852740.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7958..8332)
                     /locus_tag="ID725_RS21345"
                     /old_locus_tag="pgaptmp_004269"
     CDS             complement(7958..8332)
                     /locus_tag="ID725_RS21345"
                     /old_locus_tag="pgaptmp_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGV7852741.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8495..8716)
                     /locus_tag="ID725_RS21350"
                     /old_locus_tag="pgaptmp_004270"
     CDS             complement(8495..8716)
                     /locus_tag="ID725_RS21350"
                     /old_locus_tag="pgaptmp_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGV7852742.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8779..9255)
                     /locus_tag="ID725_RS21355"
                     /old_locus_tag="pgaptmp_004271"
     CDS             complement(8779..9255)
                     /locus_tag="ID725_RS21355"
                     /old_locus_tag="pgaptmp_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGV7852743.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9271..9756)
                     /locus_tag="ID725_RS21360"
                     /old_locus_tag="pgaptmp_004272"
     CDS             complement(9271..9756)
                     /locus_tag="ID725_RS21360"
                     /old_locus_tag="pgaptmp_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGV7852744.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9847..10665)
                     /locus_tag="ID725_RS21365"
                     /old_locus_tag="pgaptmp_004273"
     CDS             complement(9847..10665)
                     /locus_tag="ID725_RS21365"
                     /old_locus_tag="pgaptmp_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGV7852745.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         2753 a         2725 c         2719 g         2715 t
ORIGIN      
        1 ttttatattt tacccattta gggctgattt attactacac acagcagtgc aacatctgtc
       61 agtacttctg gtgcttctat tttagaggca gctgtcaggt gtgcgatcaa taaaaaaagc
      121 ggggtttcat catgtttaat gaagtccata gtattcatgg tcatacatta ttgttgatca
      181 ctaaaccttc tttgcaggcg acagctctct tgcagcacct taaacaatcg ctggcaatta
      241 caggaaaatt acataatatt caacgttctc tggacgatat ctcttcaggc tctattattc
      301 ttctggatat gatggaagcg gataaaaagc ttatccatta ttggcaggat actttgagca
      361 ggaaaaacaa caatatcaaa atattgttgc taaatacgcc tgaagattac ccgtaccgcg
      421 acattgaaaa ctggcctcat atcaacggcg ttttttatgc catggaggat caagaacgcg
      481 ttgtcaatgg attgcaaggc gtcctgcgcg gcgaatgcta ctttacgcaa aagcttgcca
      541 gttacctgat tacacattct ggtaactatc gttataacag cacggaatca gccctcctta
      601 ctcatcggga aaaagagatc ctgaataagc tgcgtatcgg cgcgtctaat aacgagatcg
      661 ctcgttcgtt gttcatcagc gaaaatacgg ttaaaacgca tctttataat cttttcaaga
      721 agatagccgt aaaaaaccgg acacaagcgg tttcctgggc aaacgataac ctcaggcgat
      781 aaagccatga aacgttattt acgctggatt gtggcggcag aatttctgtt cgccgcaggg
      841 aatcttcacg ccgttgaggt agaagtcccg ggattgttaa ctgaccatac tgtttcatct
      901 attggccatg atttttaccg agcctttagt gataaatggg aaagtgacta tacgggtaac
      961 ttaacgatta atgaaaggcc cagtgcacga tggggaagct ggatcacaat aacggtcaat
     1021 caggacgtta ttttccagac ttttttattc ccgttgaaaa gagacttcga aaaaactgtc
     1081 gtctttgcac tgattcaaac tgaagaagca ctaaatcgtc gccagataaa tcaggcgtta
     1141 ttaagtacgg gcgatttggc gcatgatgaa ttctaaataa aaaattgttc ggaggctgca
     1201 atgcgtgtca aacatgcagt agttctactc atgcttattt cgccattaag ttgggctgga
     1261 accatgactt tccagttccg taatccaaac tttggtggta acccaaataa tggcgctttt
     1321 ttattaaata gcgctcaggc ccaaaactct tataaagatc cgagctataa cgatgacttt
     1381 ggtattgaaa caccctcagc gttagataac tttactcagg ccatccagtc acaaatttta
     1441 ggtgggctac tgtcgaatat taataccggt aaaccgggcc gcatggtgac caacgattat
     1501 attgttgata ttgctaaccg cgatggtcaa ttgcagttga acgtgacaga tcgtaaaacc
     1561 ggacaaacct cgaccatcca ggtttcgggt ttacaaaata actcaaccga tttttaagcc
     1621 ccagcttcat aaggaaaata atcatgcagc gcttatttct tttggttgcc gtcatgttac
     1681 tgagcggatg cttaaccgcc ccgcctaaag aagccgccag accgacatta atgcctcgtg
     1741 ctcagagcta caaagatttg acccatctgc cagcgccgac gggtaagatc tttgtttcgg
     1801 tatacaacat tcaggacgaa accgggcaat ttaaacccta cccggcaagt aacttctcca
     1861 ctgctgttac gcaaagcgcc acggcgatgc tggtcacggc attgaaagat tctcgctggt
     1921 ttataccgct gaagcgccag ggcttacaaa acctgcttaa tgagcgcaag attattcgtg
     1981 cggcacaaga aaacggcacg gttgccatta ataaccgaat cccgctgcaa tctttaactg
     2041 cggcaaatat catggttgaa ggttcgatta tcggttatga aagcaatgtc aaatctggcg
     2101 gggttggggc aagatatttt gggatcggtg ccgacacgca ataccagctc gatcagattg
     2161 ccgtgaacct gcgcgtcgtc aatgtgagta ccggtgagat cctctcctcg gtgaacacca
     2221 gtaagacgat actttcctat gaagttcagg ccggggtttt ccgctttatt gactaccagc
     2281 gcttgctgga aggggaagtg ggttacacct cgaacgaacc cgttatgctg tgcctgatgt
     2341 cggctatcga aacaggggtc attttcctga ttaatgatgg tattgaccgt ggtctgtggg
     2401 atttgcagaa caaagcagaa cggcagaatg acattctggt gaaataccgc catatgtcgg
     2461 ttccaccgga atcctgacag ataaaaatgc cggaattaat aatccggcat ttactgttta
     2521 agcgaaagcg tgagtgcccc tcacgcttta tttttgattt cctgctcacg gagagccgtc
     2581 tttgcactac gcgccgccag ccacaggccg ctgtttttca ttgcgtaacc aaatatcagc
     2641 ccaagcgcca gcgaaggtaa caccagtttc cagtcaccct gcccggcaaa cgtcgcgcat
     2701 gcgcctataa acgtccccgg aacaaatgaa agcagcagct gtttggcctg aatacacatc
     2761 agaaacgcga caataccggt tatgacataa ctgagaattt ccagatgtgg cgccagtgca
     2821 ctaccgtaaa taatgaccat cgcccacacc acgccactaa gcagagttgc agcggagatc
     2881 gccagccctt tcagcccacc ttgcgggcag gcaaagtagg ccgtacagcc caggaagccc
     2941 gcccagctta gtaagcccag ggaaacagcc acccatcccc agataccgga gagaatgcct
     3001 gttgtgattg caatagagag aagtatgttc atgccgcgca tgatagcaga aaacgcggcc
     3061 atgaatgaga tcgagcacac attttatgca acagcaaatt atctgttgca gttatgagtg
     3121 acgcatatca catttactct tcagaatctt cttccagctc gtcccacatt gcactaatgg
     3181 catcgcgggt tagcggtgcc atggtgcgcc agaaaggcgt ggttgcatgg gcctccactt
     3241 tgccaaggaa cgcaccacac cagggtaaca gatactcact gaacagtgtt tccagtgctt
     3301 cgctctcatc ttccgttgac tgatcttcca gccaggaagc cgcaagcagc aatgtgccga
     3361 tgtgatcggc aggcgtatcc gctaatggca tccctcgttc agaaagaaaa gcgcgcactt
     3421 ccgcttccgt tgcgtcctca acccatgcgc tacgatatgg cggcacggca cattcatcgc
     3481 cgataaacaa cgcattgtaa tcggcagaga cttgcgtcat atcacagctt ttctgcaaac
     3541 gtgtcagcaa ctcatcctgc tccagtggcc agttcgcagc cagtttcccc tcacgaatca
     3601 gggtaaacag cggcaccagt aaaggatctt gcggttggcg gtaatagagc gaacccagca
     3661 cacgacagag gatagaaaac tcgttcatta atatatttcc attactagta attaaaggtc
     3721 tgcaaattcc gcaatcggtg ccataccgcg agattcaagg aagttcagta agcggcgcgg
     3781 agaaacattc aaaatgcgct ctggcggaaa atctaccgcg tcgaggattt taagacactc
     3841 ttcaaattcc cccatggtaa acgcggtgtg agaatccgag cctaatgcca cccaacctcc
     3901 agcatcacgt accgctgcgg ctaccgcacg acagttgtct tcactgccct tacgtgagtg
     3961 taaaaatgag gaattattga tttccagcgc cacctgatgt ttcgcggctg cttcagcaac
     4021 ggctttaaca tctatctcat atttcggatt tccgggatgg cttattatat gcacattgcc
     4081 gctggcgata gtggcgatca tcgcttgtgt attggtcgct ttgtcatgtg gtgcaaaaac
     4141 cggctcatga aaaccggcaa taatcagatc cagcgagtca aacattttac cgctgcaatc
     4201 aatttcacca tcaacatttt taatgttagc ttcgatgccg cgcaggatcc ctaccccatc
     4261 aaccactcgc ggccagatac gcatgttaat gaagtgccag tgatgcggcg catcttccat
     4321 gtccgggcca tgatcggtaa tcgcaaaaag tttaatgccc ttttgtttgg cctgggcaat
     4381 gtaatcactc aatgtgctat atgcatgtgt gctggcaacg gtatgcatat gaaggtcgac
     4441 gggatacata actctctcct gtattcaatt cccgccaagg atagcaggaa tcctgatgct
     4501 ttattagtag ccgcgtgcgc ggtcgacttg cccgcagacc ctctcccctt tttcgagctg
     4561 ggcaatagtg cgagaaatgt actccacagc ttcagcggga cgggtaatcg cggcgacatg
     4621 tggtgttatc gtcacgcgtg gatgttgcca gagcggactt tcaggcggta agggttcacg
     4681 attaaaaaca tccaacattg cgcctttaac tttgccgcta tccagcgccg cgagcaggtc
     4741 atcttccacg acatgaacac cacgcgccag gttgaggaga tacgcgccat ccggtaattt
     4801 ttcgagtaat tgttgattaa taatgccgac ggtttcaggg gtattcggta acaaattaat
     4861 caatacccga cattggctca gaaatgcaga cagttcttcc catccggcaa agctttgcac
     4921 gccaggccac gatttacggg ttcgactcca gcaacgcagc ggaaagcgcc aggtttgcag
     4981 actctgagca actttactgc ccagtacgcc tgcgcccaaa atgccgatgg taaaatcttc
     5041 ccgatgatat tcaggcagcg gttgccaatg cgaactattt tgctggatgc gataatcgtc
     5101 aaaacgtcga aaccaatgca gcacctgact gacagcatat tcctgcattt gctcgcccat
     5161 accggtatct tccaggcgaa aaagtggaac agaaggcttc agcatttcag ggtgtgcctg
     5221 tagcttgctc aaaatagaat caacaccggc cccgagtgcg aacaccgctt taagatcgcg
     5281 ccctgccagc atttcaacag gaggatgcca ggctaaagca taatcagcag agtcattatc
     5341 tccgcttttc catgctctga ctcttgcctg aggaatagct ttgcgcagtg cctctatcca
     5401 ccattgggta tcgaacgttg ggtgataaaa gatgatatcc atactgactc ccgaaaagcg
     5461 ttgtgcgaaa tttattcgca cttatcgtta tgatctacaa agaccaccag cataacaaat
     5521 ccgtggtcgg tggccaaaaa agcagatttt gcttattaaa actgcatatt gattgaagtt
     5581 tgaataaacg cgcgattttt taaaaaagtt tgttgacctc aggtcatgat ttccctaaat
     5641 tagcgcccgt tccagcaaga caggaacgac aatttggtga ggtgtccgag tggctgaagg
     5701 agcacgcctg gaaagtgtgt atacggcaac gtatcggggg ttcgaatccc cccctcaccg
     5761 ccagattata agcgcatgtt attgtttaac atgcgctttt ttattttccc ttccagacag
     5821 tatgccatcg ggtatgccat ctgaaaccgg tttatatgcc ccgcgatcat atcttatcgg
     5881 acaatttccg atctttgagc atactggcaa agctatactg ccagcatatc aggatagtgt
     5941 tttgcgataa tatcattcag tctgtcaacc agttctggtt ttctgaacgt gatatgcgcg
     6001 gagcccttct taaagtatct gatgctgaac atctcatctt catagctctc tttaccctga
     6061 acagactgaa tgtgatcatc cagacgaagg gtgatgtttc cccggttgtc ggggattggt
     6121 ttaccgctga acaggtgcaa cattctttcc agatcggcga gtcggtcagc ctgttgtccg
     6181 gtgataagat gaaatcccca ccggtcccat ctcaccagat tgttgacgat aattttactg
     6241 ccaaatttac aggggcaatt ggttttgtaa ttccagttca gccctctgaa gacgttgatc
     6301 acccctcgct caaacacctc atccttattc tggtgcagtt gttcaaatgt gctcagaatg
     6361 ttcgcttcgc tgatttccgg aaaattatcg tactccagtg accggtacca tgtatcacgg
     6421 gcctgcgtat ccattaatga cagcatcccg gaacgttgca tcaggtcacg ccagatttcg
     6481 cgatcaagat tccgggtaat ggctttcatt gctgtttcgg gtttctccat cagccagcag
     6541 ccgcagcgaa aatcctgttt catggcccag tccggggcag ttttacctcc gatgctttcc
     6601 gtcagcaccg agatttcagc cagttgtcgg ataagtgtgt cgatttgcaa cagtgtactc
     6661 tttcgtacag ccagaatgtg gcgaatgctg gttgagcaga taacgtccgt atgcccgttg
     6721 accacttccg gacgggagat tgtgatatct gacataatcg atattccttt ttgcagataa
     6781 atgaaaacgc tagccctgtg caggctggcg tcagaggaat gaagtctggt gagtgagctt
     6841 caccggttct ggtgcagaag ctgcagatga cgcagggtac ggacatcctc ccgggcctgt
     6901 tcgatacagg gcaacagtgc tgctgaatcc gttttatcct catcgttgtc aaagataatt
     6961 cccgatccgc agtcgatatt gtcctgcagc cacgcaatca gaatatccag cgctgtttcc
     7021 gtggttaatg atttcatgtt gttcctggtt accagtcgaa agtgggtaaa cccggcaggc
     7081 attcagcgct ggcatccaga tgaatgagac tgacgccata acgccggatg agtgtgacga
     7141 ccagacgacg gaacgttttt gacagaccca ggcgtttaag ccgcagaacc gggtacgacc
     7201 acgcatcggt acgcaacaga tagccgttac cggtaaaatg aatccattct gattcaccga
     7261 agtcactggt ctggtgtgac agcgagtaca gccaggcgtt gtccgtttcc gtgatatgtg
     7321 cggtactgca gcgtatgccg gtaagggtag caaatggtgg cgtgcattct gctgaggtct
     7381 ggtcagattc atcaacaaca cgaagggagt agccgttatc gcagaccatg ttaatcagtt
     7441 cagcgagctc aataccatcg acgtcaacga caatgcgccc catgttcagt gcctgtacgt
     7501 taacgctgtc ggcttccagt gtcagggcca gtttcatcat ttcgcctccg gatacttacc
     7561 cagggtaatg ttatttaccg ttctgtaatt gtcgcgggtc atcaggccgg tcgcccggcg
     7621 agcccggagg atatcaatac tgtttattaa ctgagagcgg ggacaggtgc tgaatcccgg
     7681 ctggtcggta cgcaccagcg cgtatttttc cacgagaaaa ttaactgcat cacacagtga
     7741 aatgcctgcc tcaatatgct gctcaatcac acgttcatcg gcaaacggtg tgtcattcag
     7801 tgtgaggcca taatgctggt ccagcagtcg gatgagcagt gtctgccaga tggtgacggg
     7861 agacgggcag cgagatacct cccgtacatg tgtgtcggat aatgttttca tcttaagttc
     7921 tctgtaactg gtagtgctaa aggtgagagc ttcggggtta tgaggtggtt gcgggtgctg
     7981 gtgtcggata aacagccaga taaacgtaac cacaggagcc gagggtgtcg gcttcgcagg
     8041 tcagcccttt tgcattcagc gtgacggtat gctgatatcg gggattcagt tcaccgctgg
     8101 tgagcatgag ttccagttgt ttcatcagca gcggaaaggc ctggtccaga tggtatgcat
     8161 ctgcgtcgct gaagcggcct ctgataccgg cgcggtcggc aaggtaatgc aggcggttac
     8221 cctcttgcac cagacgagcg ccaaaacagg gcctcactgt gcagggaagc ccccaccagg
     8281 ggcggtcgtg attgtcatcg ggatgagtta tcccggagag tttgtctgac acgataaaat
     8341 cctctctgaa aagtggttaa gaatggttta acgatgacag gtcagccggc gcgctgaccg
     8401 gtttcggtga agatgtatgc attgatgaga atggcctcat ccacagcggc gtctgaggtc
     8461 atctggtcgt actcgtttcc cgggtgacgg aacatcagtt cagggtgaca ctcatcagcc
     8521 ggacatacgg gccaaaactg tccttacggc gttctgcaaa cacggcaaga acgccgggaa
     8581 tatcctgcac ttcacgacca gtatacgctt cagcactgcc gtgccagcga tacttaccgg
     8641 tacagaacgg aaaaagacgg gatgcaggat gctgttggtg aatacgcatg gcttcaccac
     8701 gggtgatgat tctcatgatg tgatacctct gaagacagaa gataaaagtg aaaacaggtg
     8761 tgatgtggtt gtgacgggtt aaagcagacc atgttcggca aaggagaaaa cctggttgcc
     8821 accgactatc agatggtccg gcacccggat atccaccagg cccagtgcct gtaccagacg
     8881 ttcggtgata agccggtctg ccttactggg tgtgacttca ccggacgggt gattgtgcgc
     8941 cagcaccacg gcagcggcat tgtggtacag ggcgcgttta atcacttccc ggggatgaac
     9001 ttccgtacgg ttgatggtgc cggtgaagag ggtttcaccg gcaatcagct gattctggtt
     9061 gttcagatac agcacccgga actcttcccg ctccagtccc gccatgttca gaatcagcca
     9121 ttcccgtgcc gcacgggtgg aggtgaaggc cacgccgggt tcatgaagat ggcggtccag
     9181 ggtttgcagg gcccgcagaa tgagactgcg ctcgccgggc gtcatctctc cgggcagaaa
     9241 ggaaagctgc tgcattgtcc ctgcctccat tcagtcaatg atgcgcataa tggcgctgca
     9301 ttccggatgc tgcagggcgt aatcccgcag ccggtaatag tggaccgtca tggcataaca
     9361 ctccgtacga caggcatgat gactgtacgt catcagacag gcagcaatac cggcggcttc
     9421 cgggctcatt tcagcgcggt tgccgttcat ggcattgaac agtacccatg tttcgtcatc
     9481 atcgtcatcc ggttcgggtg ccataaatgc cccgccgttg ttcagggtgt acagattcca
     9541 gataccaccg cagtagtctt cgcaaagacg gtccatccag ccgaagacac ggggctccag
     9601 ggtcacccac tgtggaatga ggccaaaatg ctgcggccag aagctgatgc gctgttcatc
     9661 agggacggga gtggcaacca gctgaggtgg gttattccct gatgcagtgg ttacggaaac
     9721 agaaggtgtg gtggaattat gcgaaacggt tgtcatgagg ttattcctta taaaaagtaa
     9781 atgaatggaa gaagccccgg ggcaggaaca gatgtgagtc aggactgcac tttcagtgaa
     9841 acggcatcag cgcatgctct ccagcagcgt ttctgccatc acccacaatg cgcgattgag
     9901 cttaatgtcg gtatcaatgc tgtgaatggc ccgggtatgg atacgttttc cttttgcact
     9961 gcgaccggaa atcccgcctt tcagcatatt ctcctggatg gtctgataag cactccacag
    10021 gtccttaccg taatcctccc ggcgtcgtgg tgtcagaatg tcggcggtgg tgacgggctg
    10081 atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg
    10141 cggcaggacc agcgactgca tggcatcacg cttctcctct atccggtcaa aaacgcccac
    10201 cacctcgtaa gccccttcaa tgactctgtc caccacattt ccccggtgtg gaacacgcaa
    10261 ttctcccaga gactgaccgc agaccagtcc attggtacaa atggcacgaa aatatccggg
    10321 taacatctgg tagctggatg aaccgtcatg ggagttgagc agaataattt cagggacatg
    10381 ctgaccggtt atctgtccgg cccgccgcag acgcagcatg tgttttgtgt attcccggcg
    10441 gctctggtcg cgcacgcggg tctggcaggc aaagaacggc tgaaagcctt cctgctgcag
    10501 attttccagg acagtgatag tgggaatgta cgcatagtgt tcactgcgag aggtatgtcg
    10561 gttttcccca aaaatactcg gtacatggcg catcagttct tcgtgtgtca gcggacgatc
    10621 acgacgtatc tggttcgcat aaccaaaacg actggctaat ctcatgattt gctccttatg
    10681 aatgattaag cgatataaat gaaaaagccg cgtctcccgg agaagacgcg gcctgatggg
    10741 tgaaatgaat gaggtttatt gtctgagaag cccttcactg gcgaaccacg tattaagctg
    10801 tattccggca tcgccagtgc aactgacctt cagcattgcg gataacccgc cggaaatatg
    10861 ttcactggtt gtttttgtat cttcccccaa aattaaataa attcatctta ac
//
LOCUS       DABSYT020000132        10588 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.132, whole genome shotgun sequence.
ACCESSION   DABSYT020000132 DABSYT020000000
VERSION     DABSYT020000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.132"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(106..1107)
                     /locus_tag="ID725_RS21370"
                     /old_locus_tag="pgaptmp_004274"
     CDS             complement(106..1107)
                     /locus_tag="ID725_RS21370"
                     /old_locus_tag="pgaptmp_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7852746.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(1113..1460)
                     /locus_tag="ID725_RS21375"
                     /old_locus_tag="pgaptmp_004275"
     CDS             complement(1113..1460)
                     /locus_tag="ID725_RS21375"
                     /old_locus_tag="pgaptmp_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HGV7852747.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(1490..2140)
                     /locus_tag="ID725_RS21380"
                     /old_locus_tag="pgaptmp_004276"
     CDS             complement(1490..2140)
                     /locus_tag="ID725_RS21380"
                     /old_locus_tag="pgaptmp_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852748.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHIESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(2156..2560)
                     /locus_tag="ID725_RS21385"
                     /old_locus_tag="pgaptmp_004277"
     CDS             complement(2156..2560)
                     /locus_tag="ID725_RS21385"
                     /old_locus_tag="pgaptmp_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGV7852749.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(2650..2787)
                     /locus_tag="ID725_RS21390"
                     /old_locus_tag="pgaptmp_004278"
     CDS             complement(2650..2787)
                     /locus_tag="ID725_RS21390"
                     /old_locus_tag="pgaptmp_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852750.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            2859..3062
                     /locus_tag="ID725_RS21395"
                     /old_locus_tag="pgaptmp_004279"
     CDS             2859..3062
                     /locus_tag="ID725_RS21395"
                     /old_locus_tag="pgaptmp_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGV7852751.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            3084..3434
                     /locus_tag="ID725_RS21400"
                     /old_locus_tag="pgaptmp_004280"
     CDS             3084..3434
                     /locus_tag="ID725_RS21400"
                     /old_locus_tag="pgaptmp_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HGV7852752.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            3445..3723
                     /locus_tag="ID725_RS21405"
                     /old_locus_tag="pgaptmp_004281"
     CDS             3445..3723
                     /locus_tag="ID725_RS21405"
                     /old_locus_tag="pgaptmp_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852753.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGIVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            3735..3977
                     /locus_tag="ID725_RS21410"
                     /old_locus_tag="pgaptmp_004282"
     CDS             3735..3977
                     /locus_tag="ID725_RS21410"
                     /old_locus_tag="pgaptmp_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HGV7852754.1"
                     /translation="MGKEYKTLINKAPERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            3974..4087
                     /locus_tag="ID725_RS21415"
                     /old_locus_tag="pgaptmp_004283"
     CDS             3974..4087
                     /locus_tag="ID725_RS21415"
                     /old_locus_tag="pgaptmp_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852755.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
     gene            4180..4596
                     /locus_tag="ID725_RS21420"
                     /old_locus_tag="pgaptmp_004284"
     CDS             4180..4596
                     /locus_tag="ID725_RS21420"
                     /old_locus_tag="pgaptmp_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852756.1"
                     /translation="MNAKEKNIINTLKIVSAEQDKLSRAAQKDNQHMAALYALTIAIA
                     TPEAAKVIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            4620..4823
                     /locus_tag="ID725_RS21425"
                     /old_locus_tag="pgaptmp_004285"
     CDS             4620..4823
                     /locus_tag="ID725_RS21425"
                     /old_locus_tag="pgaptmp_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852757.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            4820..5086
                     /locus_tag="ID725_RS21430"
                     /old_locus_tag="pgaptmp_004286"
     CDS             4820..5086
                     /locus_tag="ID725_RS21430"
                     /old_locus_tag="pgaptmp_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGV7852758.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            5083..5382
                     /locus_tag="ID725_RS21435"
                     /old_locus_tag="pgaptmp_004287"
     CDS             5083..5382
                     /locus_tag="ID725_RS21435"
                     /old_locus_tag="pgaptmp_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852759.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            5705..5935
                     /locus_tag="ID725_RS21440"
                     /old_locus_tag="pgaptmp_004288"
     CDS             5705..5935
                     /locus_tag="ID725_RS21440"
                     /old_locus_tag="pgaptmp_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HGV7852760.1"
                     /translation="MADRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHEFQQLIQQQNKLH"
     gene            6008..6373
                     /locus_tag="ID725_RS21445"
                     /old_locus_tag="pgaptmp_004289"
     CDS             6008..6373
                     /locus_tag="ID725_RS21445"
                     /old_locus_tag="pgaptmp_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852761.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATACRDTLNGMTK"
     gene            6380..9202
                     /locus_tag="ID725_RS21450"
                     /old_locus_tag="pgaptmp_004290"
     CDS             6380..9202
                     /locus_tag="ID725_RS21450"
                     /old_locus_tag="pgaptmp_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGV7852762.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRQKGKKSWQAERYARGNLRKIFERLERTDHRWLTQGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMTESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFTTYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPGVEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRRDI"
     gene            9279..10238
                     /locus_tag="ID725_RS21455"
                     /old_locus_tag="pgaptmp_004291"
     CDS             9279..10238
                     /locus_tag="ID725_RS21455"
                     /old_locus_tag="pgaptmp_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGV7852763.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVATAIREACGVNATFIADGVPQFALVNGLYAMNKE"
     gene            10243..10557
                     /locus_tag="ID725_RS21460"
                     /old_locus_tag="pgaptmp_004292"
     CDS             10243..10557
                     /locus_tag="ID725_RS21460"
                     /old_locus_tag="pgaptmp_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGV7852764.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQANQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT         2950 a         2835 c         2434 g         2369 t
ORIGIN      
        1 actatcaacc accatttata aatcataacc ttatgatttt attaactttg aaaatggctc
       61 tatacaacgc catttgaatc tattgtcact ttttatcgcc actttttacc ctctattgtc
      121 aaaagggttc aattcaacag cagcctctaa atgacctgga gcaaaatgcg cataacgcat
      181 agtcattttt atatcgctat gccccagtat tttttgcaac acaagaatat ttccaccccg
      241 catcataaaa tgactggcaa acgtgtgacg tagcacatga gttaattgcc catcaggaag
      301 ctcgatcttc gctctcttaa ttgcagcgtc aaaagcctca taacatggtg aaaatagcgc
      361 tcctcgtttt ttaggaagca tagcttgcaa ttgaggtgaa atcggtacag tgcggttctt
      421 ctttccttta gttttaacaa atgtgattcg accgggcagt acttgagatt gctttaatcc
      481 ttctgcttca ctccaccgag caccagtcgc aagcccaata cggacaacaa cccccaaatc
      541 tttattccgt gactcatcac acgcaatcag aaggcgttca atctcatcta catacagaaa
      601 cgccagttcc ttttcttcct cacgaaactt gcgaatacca gtcagggggt tttcaccaga
      661 ccactcccca aggcgcttca gttcggcaaa aacagcatgt agatatgact gctcgcgatt
      721 aacggttgct tcactaagtt ttttcttccc cttgggattc cattctcctg atagccttct
      781 ttcccgataa gtagcaaaca tatttttgtc aaactgagaa gcaaatggat ctcccagcct
      841 ggaacaaatc gcctcaagtt tgactttgcg ctctgcacca gaggacaagg ttttaccgta
      901 catctcaaac caacgagcaa tcaactcaga aagacgagga ccagaaccat cttgaaactc
      961 gtctccaact ctactattca ttaaacggcg ctcataagag agcgcctcac tttttgtcgc
     1021 aaactgttta cgaatgcgtt ttcccgatgc cccgtaggga taacattcac aaagccattt
     1081 acctgatgga atcttacgaa ccgacatttt agttacttat cacataaatc aaatgcagcc
     1141 ttagtgacat cccccagact cttttttaac cctggggcgg catcattatc tagccaaaat
     1201 ggattattgt tatctaacgg taacgcacca aatgttttac cttttattcg agccaaacct
     1261 gtaagtgcat ataacttatt atcgtcaaaa ttcatcacat aaggattacc atcaagacac
     1321 tgtaattgaa cctcatcagt attaaatggc cataccccat tgaaactctc acgttcaata
     1381 gttttaaaag gcattgcgac ggcggaaaaa gaaaacatag ataaaaaagt aactaatagt
     1441 tgaacctttt ttactttcat atcattacct caatttagct caagtaagat tacaaattaa
     1501 aaaacgccct agaaatgaca ccgcctacca aaacccctac gcagataaag aatattattt
     1561 cttttggata aagtcggatt aattctgaag cacgaagtcg gacttctggt aaggtcgaac
     1621 tctctatgtg gcttgatgcc gattgttgct ctaaccacga caatgcagac tgtaactgag
     1681 aacgagtaag atcgtttaaa cgtcctgtac cgaaattgat atggcaatac cgcagaagtt
     1741 tttgtcgaag tccacagtct tcactgttac gtagtaataa acttacaaga gccttacagg
     1801 catcatgatc tttacatcgc tcaagcattg catgcagaaa actctccgct gttttatatt
     1861 gatttactgt catatcatca ataccagcta caccaatctc cgcatgtact ttttgccaaa
     1921 taataaacgc ttcagtattg ctagcttctg caatagcagc aaccaagcta tttagctcct
     1981 tacgctgagc cttaagcaaa gggcgatcgt catcatcatt attcgaaggg attgcgatat
     2041 tgacggtatg agaaccatca tatcgctcta tctgaatatt cttttcgtga aaatcacgcc
     2101 cagcaacgcg attgtttgaa ccgtttgagt tgacggccat gtcacctccc tactatcacc
     2161 tacccttagt ttcgttatag tcacgaccag cgatacggtt attaccacca gaaatattta
     2221 actcacgtcc tgatggctga gtttcctttt cactgatcgc accttttaaa gccccaatca
     2281 ccgcgttttt cacatctaac gaagctgctc gaaagcgagt aatcaattcc tgctcatcat
     2341 cgttataagt ttcaggtgag tgaattccca acacaacata ctgaacatca aggccaaaac
     2401 gagacagcgc tgccaaatac gcagcatcag gaaagctatc tcctttctca tatctaagct
     2461 gagttagctt tttgactcca ccaatgtcgc tcatggcaac ttgactaagt cccaatcttt
     2521 ccctttcctc acgcaaccgc cgaccaatat catttttcat acaaaaacct tgacaggtat
     2581 cttttttgat accaaaatga tttcacgagc tattagatga tcacaatata ccactatgaa
     2641 acaagttctt cacgatacca gatcacgcat tccgcgtaac accgccacag gtccaagact
     2701 ggcacttcgg ctgtccctcg aggagcgagc cgtcattgat gaaatggcag ctaaagaaca
     2761 acgctcatcc tctaacatgg cgcgcatgat ctcccttcgc ggcctagagc taacccagaa
     2821 agaacaaaac aaatcttcct gatcaggagg ctagtgggat gtcaggtata accatcaata
     2881 tcaatgtgaa tgccccctat gtatccctgc agaaatatgc agagataaca ggtatccctc
     2941 ttaatacatg caaaaagatg ttggctgacg gtcgaattat tatccgcccc aaacgcgcca
     3001 aaatggaaaa gcctgaagta aaccttgtgg cgatgttaaa agacgctttg gctaacagct
     3061 aaaacaatga acagagcacc atcatgaaaa aaaacgctaa taatccatac tccaaatttc
     3121 gtaatggcgt agaacgccat gtacaccacg tcgctaccag tgcatcacgt agtaacagtc
     3181 gctataacct gaacgagacg catgcaacac cggatggcca cgctgtaaaa caaatcggcg
     3241 aacatgcctg gctgattgag aaagctggaa tcgtggtcca caaatgccca cgcaatccgt
     3301 ttaccgggaa ccgcattttt gcattgaact gcggcgacaa tcacttcggg caggatttca
     3361 cattatacga agcacttcgc acggttgatc gtctgcttcg cgggcaaagt tttattaaac
     3421 aggctgattt ataacaggtg ctttatgacc aaagagcatg cacaaggtgt atttatccgt
     3481 tttattgatt ttcgcggtga actgttatta cgcgcatcag ccattgacgg aattgttcca
     3541 tccgaaaaaa acgcagctac ttacgtttat ctgaacggta cgcgcctgac tgtggagctt
     3601 ccgtaccaga ccgtacgaga aatcattagc gaagctgaaa aggctcgtca ggttaatggc
     3661 gatgaaccct atatcgaaat tatttgtatg gattcagaag ctgaaattca gaaagcagat
     3721 taaagggcgt tgcgatgggc aaagaatata aaactctcat taacaaagca cctgaacgat
     3781 tttattttcg cttaagtgca tcaggcgctc atgctgaacg tgcagcccgt gactcattga
     3841 ccagggcaat ccggagtttg tatgacgttg ctttttacgc tgatgatctg gatgcactta
     3901 acgaactttc cgagctgatc tgtgccgcag aatgcgggga acacattgaa ccatataagc
     3961 tggggaatat cgcatgagta tatttatctc atggcttgtt ctgattattt cggtggcctg
     4021 cgccattggg attatgcgaa ttattcattc agtaaaaaag attgaacgct ttttcactga
     4081 caaataacag cttaaacaga acaccagatt aaacccgaaa acctgaaaac tatccgcatt
     4141 cgcggaggta ttcgcacacg taaataacgg agatataaaa tgaacgcaaa agaaaaaaat
     4201 attatcaata cattaaaaat agtgtcagca gagcaagaca agctgtccag ggcagcccag
     4261 aaagataatc aacatatggc agcgctttac gcactgacca tcgcaatagc cacccctgaa
     4321 gcagccaaag ttattgagga acagagcaaa gaaatcgaca ctcttaaaac acagtcaaca
     4381 gttgcagcca tgaatccgtc cagcattgga cgctgcattt atattcttgg ttcagcaatg
     4441 atgctgcaat acaccattat tgccgaactg cacggcaaat acctgataac gccttaccac
     4501 acaaaagagt cagagcttct gacaaatctc cgcctgatag aacgctctca agctgtattc
     4561 attgatgacg cgcaacgtgc cgtatttaac gcatagggtt actggacaaa gggggcgcaa
     4621 tggcaattaa gcattttccc gtcgttcgct ttacctccag agggcgcgaa tacgaggtcg
     4681 acgaacgcct gattaccact atcgacaaac atcgttcgga aaaggatgca caccacatct
     4741 acctcactga cggcacttac ttctgcgcca ccaacgtggc gcgagtaaat cttatccgac
     4801 aggtacagga gccacgcaga tgaccattct ggactacatc gctactcatc cggggtgtag
     4861 cggcggagag atcgccgcag cactgaatac cccaaccaca gccattaatg ctgagttacg
     4921 ccgactttgg cgcggcggct tagtcatcag aacaaaccgc agcacaggtg gtcgcgctcg
     4981 caaaacagga ggccaggctt cttaccacgt aaacccgatg ccgttcgggt gtagcaatcc
     5041 acttactcac atgtttaacc agctactgaa ggaagccaga gcatgagcac catcaaccac
     5101 cagaagctac gcgaactggc atttgccctg caacgaatgg caacgcctca aaaattactg
     5161 gcatttcgcg caatgctctc gccgtctgct gtgctggcac tgctggatca gctggagcac
     5221 gccagaacca cggctcctgc cattcgcctc acgctccatc atgaaatcgc tgatttctgc
     5281 gcaacgctgg gggcacctgg cgaaccggaa acgccggaag caatgcagca agagctgctg
     5341 caacgcatcg ataacgtttt tgattttttt ctgaaccagt aagaaaccag aacatgcaca
     5401 cacaaaaaaa ccgcttgcca tgccgcaatc ggtcaggtta catttccgct gcacctcata
     5461 aaacgggtgc cgggattctc aacccgatac agagcaaagc gcataaccgc gctagcggtt
     5521 tttttgtgcg tactgtattg ccacgtcttt ttcgcgtcag aattatggcg gggcgtacgg
     5581 ggccgacttc ggtcgggccg gattctttgc tctccggtgt tgagaaccct gtacgtctcg
     5641 ccaccccgag attctcaact ctggatggtg agttatttct atcaccgagc aaagaggcca
     5701 caccatggca gaccgcaaac agcaccgcgc tatcgcggag cgtcgtcaca tccagactga
     5761 aatcaaccgc agactttccc gcgcatcacg cgtcgcgcaa atcatgcaca tcaatatgct
     5821 gcatgagcgc agccacgcac tatcaaacat ttattccgcc tctgttttca gctatctggc
     5881 ggatgatctg cacgagtttc aacagctcat ccagcagcaa aacaaactcc attaattcct
     5941 gttccgggcc tttcctgcac cttgcggcgg gaggccttcg cacatctgta acaagaggat
     6001 tgccgcaatg attctcgcca acgactttct tgaatacctg ctcaacacag aacgtgatct
     6061 tgccgctcgc gtgcgtgatc gttatgacat gtacctgaaa tccctgcctg taccgcagct
     6121 cgctgacgga aagattgtta ttgatggtcg ctacatgatt gacagccacg agggaaatta
     6181 caggctttac cgcattgaag gtggcacccc gtccgttatt ggcatttacc agcgcccatc
     6241 ctctgcaatc gtcgatgtga ttgccgacag catccgcatc acacatcgcc atgccgacac
     6301 agaagacacc gtgctggaaa ttcagcggct ggctacagcc tgccgcgaca ccctgaatgg
     6361 catgacgaag taaatcacta tgacggcaga gtacatcagg gactggcaac aaccgcgcca
     6421 cgcagtgggg cgtgaaggaa cggggatccc cgctcctgaa tccgcgcttt cctcctggct
     6481 ggatgcctac cgggtagaga acgagcgccg ccaggaaatg gctgatgcgg cgttctccgc
     6541 aacgccgctg ggcaacctga ttaataaaag cctggacgca caggaaaaac aggacaaaac
     6601 catcacactg gcaggagacg ccagaaaaca ggcacgcggt gcggtggatg aagccatggc
     6661 ctcgctgcgc ctgctgccgt cctatctgcg cgatccgctt attcgccacc tctccttcct
     6721 gcgcaaaaaa caggaagccg atcgtcagaa aggcaaaaag agctggcagg ctgaacgcta
     6781 cgcgcgcgga aacctgcgca aaatattcga acgtctggag cgcaccgatc accgctggct
     6841 gacacagggt tatcgctccc ttgccggacg cgaacgcctg gacgatttgc tttacctgcc
     6901 gcagctcaac aaacaccaga tacagacgct ggccaccatg acggcggcga tgttcagcag
     6961 caccttcgaa aaactctgcg atggctttgg cgcgaccgat ggcgaactga ccatggatgt
     7021 aacgctgaag gcgtatcaga tgctggcccg catggcgtta cacctgcacg ccatgcctcc
     7081 acattatgac gcactgacaa cagacaaaga ccggaggaac gaaccggaca cggagctgct
     7141 gccgggcgca atccttcgcc tgacctgtgc ggaatggtgg aaacgcaaac tgtggctgtt
     7201 acgttgcgag tggagagaag aacaactccg cgccgcctgt ctggtttcca gaaaaacatc
     7261 gccctatctg agccaggacg cgttaagcga gtttcgcgca cagcgcgaga aaacacgcga
     7321 tttcctgaaa agtttcatgc tggaaaacga agacgggttc acgattgatc tcgagacagt
     7381 gtattacgcg ggagtaagta acccggttca ccgtaaggca gaaatgatgg ccaccatgaa
     7441 ggggctggaa cttctggccg aagcccgtgg cgacaaagcg gtgtttctga ctgtcacctg
     7501 cccgtcaaaa taccacgcta caacagagaa cggtcatccg aatcccaaat ggaacggggc
     7561 caccatgcgc gactccagcg attacctggt taacacgttt tttgcggcgg tccgcaagaa
     7621 actgaaccgc gacggcctgc gctggtatgg catccgcacg gtggagcctc accatgacgg
     7681 caccgtgcac tggcatatga tggtctttgc tcatccggaa gaaatcgaca ccattgtgtc
     7741 ccacacccgc gatattgcca ttcaggaaga tcgtcacgag ctgggcgatg atattactcc
     7801 gcgctttaag gcggagtatg tcgacggctc aaaaggcacg ccaaccagct acatcgccac
     7861 ctacatcgga aaaaacctgg acagccgcgc cgtggatggc atcgacccga aaacaggcaa
     7921 accacgcgtt gaccacgaaa ccggaaaatc aatgaccgag agcgtggaac gcgccattgg
     7981 ctgggcgcgc cttcaccggg tccgccagtt ccagttcttt ggcatcccct cccgtcaggt
     8041 gtggcgtgaa ctgcgtcgcc ttgccagcca gatggcacgc aacccggaag gcccgcaacg
     8101 gctgaaggat gacgcaatgg atgcggttct tgctgccgct gatgccggat gttttaccac
     8161 ctacattgag aaacagggag gcgtacttgt tccacgcaag gactacctga ttcgcaccgc
     8221 ctacgacctc gcagatgagc tgaacgatta cggcgaacag agcgtacaga tttacgggat
     8281 ctggtcacca ctcatcgggg aatcctcccg tgtgtgcacg cacccggata actggaagct
     8341 ggtaagacgt aaaccgggag tagaagacag cgcccgcgaa aatggttttg accttcaggg
     8401 cggccctgcc gccccttgga ctcgtggcaa taactgtccc cgtgtacagg aaacggacaa
     8461 caacgggaca gaacagccgg aagaacggcc agcaccgtgg ccgcagcttc ctgacggcgt
     8521 tgacgtgaac gaatggatgc gctcactgaa acggcacgaa cgccgggcgc tgatgcgttc
     8581 gcttcgtgac aaacaggcaa aaaacagcag tgatgaaatg cagagctgga cacagagccg
     8641 caaacagcag cggcctttgc ctgataacca cgaattactc gctaaagaat ggcgggagtc
     8701 tgctgaatct ctcggcctgc atatcggtga acaacagatg cagcacctgt tacggggcgg
     8761 cagtctgtac gttgacggca gcatcattgc accgcaggga tttgaaattg tacgcaaacc
     8821 ggatacccgc ccggacagcc gaatcacgca gctctggcag cgcctgagcc gtaatcatgg
     8881 cgtaagcagc acggagatcc gccataaccc ggtcgccagc tatctggcac agctgggggc
     8941 atcagaccct gaagccgccg cacgcctggc atccacactt cagcaggacc agaacaccat
     9001 gaaaacaccc gttaccgtgc tttctgacat gctgcgcgcc atccgcgacg cagagcacgc
     9061 acagagaatc agtgaaacca ctgaacgcgc cagccgcaaa gcagacctgc tgcggggtgg
     9121 cctgaccagt ggaaacaaaa aacagacaga aacgggactc acaaatcccg taaatgagca
     9181 aaaaacgcgc cgcgatatat gaagcgcgca caaaacaggc aaaagcggga tttaaaaatc
     9241 ctgtaaccga ttaattaatc aacataagga aaagcgacat gaaaatttgt atcgacgacg
     9301 gctccaccaa catcaagctg gcatggactg agaacggcga acgccgcaac gccatcagcc
     9361 cgaacagctt caagtcggaa tggtctgcgc cattcggtgg cacgcagccc gcgaactaca
     9421 tgcttgatgg cgtgcgctat ggttttgatc cggtcagcga tcgctttgtc cagacgaccg
     9481 acacgcaata ccagtacagc gatgtgaatg taattgccat tcatcacgcg ctggtcaaat
     9541 caggcatcac accacaggaa gtggatgtgg ttgtcaccct gccactgagc gagtatttcg
     9601 atacaaacgc acagccggac atggccaaca tcaaccgcaa aaaagcgaac gtcatgcgcc
     9661 cggtggagta ccagaacggc gaagcattca ctatccgtaa cgtacgggtt atgcctgaat
     9721 ccattcctgc tggctttaag gcactggctg acatgagtcc gtttgaatcc ctgctgattg
     9781 tggatttggg cggaaccacg ctggatgtgg caaaggttca ggggcaactg gcaggtatca
     9841 gccaggtgtt ttgcgatcca cacgtaggcg tttctctgat ggccgatgcc gtactgtcgg
     9901 tgatggccac taacggtatg cgcaccagtc accacatcgc caataccatt atcgaacatc
     9961 gccatgatga agcctggctg cgccagcaca tccacaatga cgcgcattac gccagcctga
    10021 tggcggttat tcgtgaaaag gaagaaacac tgaaacaacg cgtgatccgc gcgctggcgg
    10081 ttttttcggg ttacgggcgg gtgatggttg tcggtggcgg ggcggagatt gtggcaaccg
    10141 ctatccgcga agcctgcgga gttaatgcga ctttcatcgc ggacggggtg ccacagtttg
    10201 ctctggttaa tgggttgtac gcaatgaaca aggagtaaac caatgacgac accaaccaga
    10261 cggataagtt tctatctgaa gcccgccgcc gtcaagagtg aacaggaggc gtgcaattac
    10321 ctcgatagcc tgccagcctc cgaacgcagc cgcgcgcaac gcgcggcctt tctggctggg
    10381 ctagcactca taaagcgcaa cccagcattt gcctactgga tggccgaatg gccagaggac
    10441 aacctcccct tcaataccgt tagcatcaag gaaacatacc aggctaatca gcctagcgga
    10501 gttgaatgca atttttataa aataaaaaag aatattcaga ccttattccc agaataatct
    10561 gataattgta tgtgaactaa taaaatac
//
LOCUS       DABSYT020000133        10332 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.133, whole genome shotgun sequence.
ACCESSION   DABSYT020000133 DABSYT020000000
VERSION     DABSYT020000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.133"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..67
                     /gene="tnaC"
                     /locus_tag="ID725_RS21465"
                     /old_locus_tag="pgaptmp_004293"
     CDS             <1..67
                     /gene="tnaC"
                     /locus_tag="ID725_RS21465"
                     /old_locus_tag="pgaptmp_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HGV7852765.1"
                     /translation="LHICVTSKWFNIDNKIVDHRP"
     gene            288..1703
                     /gene="tnaA"
                     /locus_tag="ID725_RS21470"
                     /old_locus_tag="pgaptmp_004294"
     CDS             288..1703
                     /gene="tnaA"
                     /locus_tag="ID725_RS21470"
                     /old_locus_tag="pgaptmp_004294"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGV7852766.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1794..3041
                     /gene="tnaB"
                     /locus_tag="ID725_RS21475"
                     /old_locus_tag="pgaptmp_004295"
     CDS             1794..3041
                     /gene="tnaB"
                     /locus_tag="ID725_RS21475"
                     /old_locus_tag="pgaptmp_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGV7852767.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3173..4348
                     /gene="mdtL"
                     /locus_tag="ID725_RS21480"
                     /old_locus_tag="pgaptmp_004296"
     CDS             3173..4348
                     /gene="mdtL"
                     /locus_tag="ID725_RS21480"
                     /old_locus_tag="pgaptmp_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGV7852768.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4323..5282
                     /gene="yidZ"
                     /locus_tag="ID725_RS21485"
                     /old_locus_tag="pgaptmp_004297"
     CDS             4323..5282
                     /gene="yidZ"
                     /locus_tag="ID725_RS21485"
                     /old_locus_tag="pgaptmp_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGV7852769.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5439..6188
                     /gene="yieE"
                     /locus_tag="ID725_RS21490"
                     /old_locus_tag="pgaptmp_004298"
     CDS             5439..6188
                     /gene="yieE"
                     /locus_tag="ID725_RS21490"
                     /old_locus_tag="pgaptmp_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGV7852770.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6210..6776
                     /gene="yieF"
                     /locus_tag="ID725_RS21495"
                     /old_locus_tag="pgaptmp_004299"
                     /gene_synonym="chrR"
     CDS             6210..6776
                     /gene="yieF"
                     /locus_tag="ID725_RS21495"
                     /old_locus_tag="pgaptmp_004299"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGV7852771.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6830..8167)
                     /gene="adeP"
                     /locus_tag="ID725_RS21500"
                     /old_locus_tag="pgaptmp_004300"
     CDS             complement(6830..8167)
                     /gene="adeP"
                     /locus_tag="ID725_RS21500"
                     /old_locus_tag="pgaptmp_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGV7852772.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8333..8998
                     /gene="yieH"
                     /locus_tag="ID725_RS21505"
                     /old_locus_tag="pgaptmp_004301"
     CDS             8333..8998
                     /gene="yieH"
                     /locus_tag="ID725_RS21505"
                     /old_locus_tag="pgaptmp_004301"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGV7852773.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9135..>10332)
                     /gene="espY4"
                     /locus_tag="ID725_RS21510"
                     /old_locus_tag="pgaptmp_004302"
     CDS             complement(9135..>10332)
                     /gene="espY4"
                     /locus_tag="ID725_RS21510"
                     /old_locus_tag="pgaptmp_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector EspY4"
                     /protein_id="HGV7852774.1"
                     /translation="IERRSRNLKLDDNFSKCVESIKKGTDSSFSKKNTENNNVFFKNS
                     NLIGANLGYYYSGDRCKDCAIDPNTNYSKDGLTAQKYMYMDLDGAISKEQIPDFLKKV
                     SAINIDVINFYNSESKTKEKYKNAFLELESFLSTLYVENKSYEEKYHFDNSRVEFFIF
                     KDMQENAQNIINNMIESDRIKFVESIINKMIPPPDGTILTENPKEYVQKQIKESHKES
                     ATNVTFDYKELAPIFEGVEEKQIKALSNQLEHIKSFKTDYDSRLNKYARDFYYFSSAF
                     AINWEDLLKNYQEIRASIKDYDDLLREIKELIISRNKSLEDKRTLFDNKRDNWNSIEV
                     QDEVNALNAKIVDCDDKIRSKLTIVRNNRLENQYKDDKNISDAMRNILDWFERYPDIV
                     QNITQA"
BASE COUNT         2587 a         2507 c         2439 g         2799 t
ORIGIN      
        1 cttacatata tgtgtgacct caaaatggtt caatattgac aacaagattg tcgatcaccg
       61 cccttgattt gcccttctgt agccatcacc agagccaaac cgattagatt caatgtgatc
      121 tatttgtttg ctatatctta aatttgcctt ttgcaaaggc catctctcgt ttatttactt
      181 gtgttagtaa atgatggtgc ttgcatatat acatggcgaa ttaatggata ttgcagatgc
      241 aatattcaca gggatcactg taattaaaat aaatgaagga ttatgtaatg gaaaacttta
      301 aacatctccc tgaaccgttc cgcattcgtg ttattgagcc agtaaaacgt accactcgcg
      361 cttatcgtga agaggcaatt attaaatccg gtatgaaccc gttcctgctg gatagcgaag
      421 atgtgtttat cgatttactg accgacagcg gcaccggggc ggtgacgcag agtatgcagg
      481 ccgcgatgat gcgcggcgac gaagcttaca gcggcagccg cagctactat gcgttagccg
      541 agtcagtgaa aaatatcttt ggttatcaat acaccattcc gactcaccag ggccgtggtg
      601 cagagcaaat ctatattcca gtactgatta aaaagcgcga gcaggaaaaa ggcctggatc
      661 gcagcaaaat ggtggcgttc tctaactatt tctttgatac cacgcagggc catagccaga
      721 ttaacggctg taccgtgcgt aacgtctata tcaaagaagc cttcgatacg ggcgtgcgtt
      781 acgactttaa aggcaacttt gaccttgagg gattagaacg cggtattgaa gaagttggtc
      841 cgaataacgt gccgtatatc gttgcaacca tcaccagtaa ctccgcaggc ggtcagccgg
      901 tttcactggc aaacttaaaa gcgatgtaca gcatcgcgaa gaaatatgat attccggtgg
      961 taatggactc cgcgcgcttt gctgaaaacg cctatttcat caagcagcgt gaagcagaat
     1021 acaaagactg gaccatcgag cagatcaccc gcgaaaccta caaatatgcc gatatgctgg
     1081 cgatgtccgc caagaaagat gcgatggtgc cgatgggcgg cttgctgtgc atgaaagacg
     1141 acagtttctt tgatgtgtac accgagtgca gaaccctttg cgtagttcag gaaggcttcc
     1201 cgacgtatgg cggcctggaa ggcggcgcga tggagcgtct ggccgtaggt ctgtatgacg
     1261 gcatgaatct ggactggctg gcttatcgta ttgcgcaggt gcagtatctg gtcgatggtc
     1321 tggaagagat tggcgttgtc tgccagcagg cgggcggtca cgcggcattc gttgatgccg
     1381 gtaaactgct tccgcatatc ccggcagatc agttcccggc acaggcgctg gcctgcgagc
     1441 tgtataaagt cgccggtatc cgcgcggtag aaattggctc tttcctgtta ggccgcgatc
     1501 cgaaaaccgg taaacaactg ccatgcccgg ctgaactgct gcgtttaacc attccgcgcg
     1561 caacatatac tcaaacacat atggacttca ttattgaagc cttcaaacat gtgaaagaga
     1621 acgcgtcgaa tattaaaggg ttaaccttta cctacgaacc aaaagtattg cgtcacttca
     1681 ccgcaaaact gaaagaagtt taattaatac tactgagtgg ctataaggat gttagccact
     1741 ctcttaccct acatcctcaa taacaaaaat agccttcctc taaaggtggc atcatgactg
     1801 atcaagctga aaaaaagcac tctgcatttt ggggtgttat ggttatagca ggtacagtaa
     1861 tcggtggagg tatgtttgct ttacctgtgg atcttgccgg tgcctggttt ttctggggtg
     1921 cctttatcct tatcattgcc tggttttcaa tgcttcattc cgggttattg ttattagaag
     1981 caaatttaaa ttatcccgtc ggctccagtt ttaacaccat caccaaagat ttaatcggta
     2041 acacctggaa cattatcagc ggtattaccg ttgccttcgt tctctatatc ctcacttatg
     2101 cctatatctc tgctaatggt gcgatcatta gtgaaacgat atcaatgaat ttgggttatc
     2161 acgctaatcc acgtattgtc gggatctgca cagccatttt cgttgccagc gtattgtgga
     2221 taagctcgtt agccgccagt cgtattacct cattgttcct cgggctgaag attatctcct
     2281 ttgtgatcgt gtttggttct ttcttcttcc tggtcgatta ctccattctg cgcgatgcca
     2341 ccagctccac tgcgggaacg tcttacttcc cgtatatctt tatggctttg ccggtgtgtc
     2401 tggcgtcatt tggtttccac ggcaatattc ccagcctgat tatttgctat ggcaaacgca
     2461 aagataagct aatcaaaagc gtggtcttcg gttcgctgct ggcgctggtg atttatctct
     2521 tctggctcta ttgcaccatg gggaatattc cgcgagaaag ctttaaggcg attatttcct
     2581 caggcggcaa cgttgattca ctggtgaaat cgttcctcgg caccaaacag cacggcatta
     2641 tcgagttttg cctgctggtg ttctccaact tagctgtcgc cagctcgttt tttggtgtca
     2701 cgctgggatt gttcgattat ctggcggacc tgtttaagat tgataactcc cacggcgggc
     2761 gtttcaaaac cgtgctgtta accttcctgc cacccgcgtt gttgtatctg atcttcccga
     2821 acggctttat ttacgggatc ggcggtgccg gactgtgcgc cactatttgg gcggtcatta
     2881 ttcccgcagt gctggcaatc aaagctcgca aaaagtttcc caatcagatg ttcacggtct
     2941 ggggcggcaa tcttattccg gcgattgtca ttctctttgg tataaccgta attttgtgct
     3001 ggttcggcaa cgtctttaac gtgttaccta aatttggcta aatccttcaa gaagccagtc
     3061 attcgctggc ttcttgcctc tcaggaaatc acttatgtcc aaatggcaac tcgcctgatc
     3121 ctccttcacc acgtatgctt tgcgtcacct tactatcagg acgctttagc ccatgtcccg
     3181 ctttttgatt tgtagttttg ccctggtttt actttatccc gctgggattg atatgtacct
     3241 cgttggttta ccgcgcatcg ccgccgatct caatgccagc gaagcgcagt tacatattgc
     3301 gttctccgtt tatctggcag gtatggcagc tgcgatgtta tttgccggta aagtggccga
     3361 tcgttcaggg agaaaaccgg tcgccatccc cggcgcggcg ctatttatta tcacttcggt
     3421 gttctgttca ctggctgaaa ccagcacgtt atttcttgca gggcgctttc tacaggggtt
     3481 gggcgcgggc tgttgttacg tggtggcatt cgccattttg cgcgacacgc tggatgatcg
     3541 acgtcgggca aaagtgctgt cgttactcaa cggcattacc tgcatcattc cggtgttagc
     3601 gccggtgctc ggtcatctga ttatgcttaa attcccgtgg cagagtctgt tctggacgat
     3661 ggcaataatg ggcatcgcgg tactgatgtt gtctttgttt attttaaaag aaacgcgccc
     3721 tgccgccccc gcagcttcgg acaaatcacg agaaaatagc gagtcgctgc ttaatcggtt
     3781 tttcctcagc cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca ccttcgtcaa
     3841 tacatcgccg gtattgctga tggaaatcat gggttttgag cgcggagaat acgccaccat
     3901 tatggcgttg accgctggcg tcagcatgac cgtttcattt tccacgccat ttgcgctggg
     3961 aatttttaag ccacgtacgt tgatgatcac ctcacaggtg ttattcctgg cggcgggcat
     4021 cactcttacc gtttcacctt cccatgcggt ttctctgttt ggtatcacgc tgatttgcgc
     4081 cggtttctcg gtaggttttg gtgtggcgat gagtcaggcg ttagggccgt tttcattacg
     4141 cgcgggcgta gccagctcga ccttaggtat tgcgcaggtt tgcggttcgt cactgtggat
     4201 ttggctggca gcggtggttg gtatcagcgc atggaatatg ctgatcggga ttctgattgc
     4261 ctgtagcata gtgagcctgt tgctgattat gttcgtcgcg cctggacgcc cggttaccgc
     4321 tcatgaagaa atccatcacc acgcttgatc tcaatctgct gctctgtctg cagctgctga
     4381 tgcaggagcg cagcgtaacc aaagcggcga agcggatgaa cgtgacacct tcggcggtga
     4441 gtaagtcgct ggcaaagtta agagcgtggt ttgacgaccc gctctttgtg aactcaccgt
     4501 tggggctgtc gcccacaccg ctaatggtca gcatggagca aaatctggcg gagtggatgc
     4561 aaatgagcaa cctgctgctg gataaaccgc accaccagac accgcgcggc ctgaagtttg
     4621 agctggcggc agaatcaccg ctaatgatga tcatgcttaa tgcgctgtcg aaacagatct
     4681 accaacgtta cccgcaggcg accatcaaat tacgtaactg ggattacgat tccttagatg
     4741 ccattactcg tggtgaagtg gatatcggtt tttccggtcg cgaaagtcat ccccgttcgc
     4801 gggagctgtt aagctcgcta ccgttagcca ttgattatga agtgttgttt agtgatgtgc
     4861 cctgcgtctg gttacgcaaa gatcatccgg cactgcatga aaagtgggat ctcgatacct
     4921 ttttacgtta tccgcatatc agcatttgct gggaacagag cgatacctgg gcgctggaca
     4981 atgtgttaca ggagctgggg cgcgaacgta cgattgcaat gagcctgccg gaattcgagc
     5041 agtcactgtt tatggcagcg cagcccgaca atctgctcct ggcgaccgcg ccgcgctact
     5101 gtcagtacta caatcaactc caccaactgc cgttggttgc tcttcctctc ccgtttgacg
     5161 aaagccagca aaaaaagctg gaagtccctt ttaccctgct gtggcataaa cggaacagcc
     5221 ataatccgaa gatcgtctgg ttacgggata ccattaaaaa tctttacgcg tcgatggcat
     5281 aaccgaatcg tatgaaacgc gacccgattt cacaataaaa tgtaaaaaag ttgtaataag
     5341 cttgtctgaa tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact
     5401 cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag
     5461 ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag
     5521 cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag
     5581 aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag
     5641 gtaaaccggt tttcagtgat aaaaatttgc cttcgttttc tatttcctat gccgggaata
     5701 tggttggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctgcagcgtg
     5761 cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca cacattttcc agcaatgaat
     5821 cgctatgggt cagtaaacaa aatgatccaa acgaagcgcg ggcgcagctc atcacgctgc
     5881 gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcaac
     5941 tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg
     6001 acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cacgattgaa aagctcagtg
     6061 tctgggagtt agatggcaaa cacggctgga aaagcctgcc ggatattcac agccgcgcca
     6121 acaatcctac cagccggatg atgcgttttg cccagctctc taccatgaag gctttttcgc
     6181 caaattgata gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg gttacgttac
     6241 tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtacccta ccgaaaattg
     6301 ctccggcgag catggaagtc aatgcgttac cttccattgc cgacattccc ctgtatgacg
     6361 ctgacgtaca gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc
     6421 gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctattcggta ccgggtgggc
     6481 tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg
     6541 tgttgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc
     6601 gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg
     6661 tgattcagaa caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc
     6721 acctgaccgg gcaattgacc gcatttggcg agtttattca gcgagttaag atctaaataa
     6781 aaaacccgcc agcaatcatg catggcgggt ttttaacgcg ctatcgattt tagtgagcgt
     6841 cgataaatac aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt
     6901 cacgaatacg cccggtacca atcttcatca cgcagtagga gataaagccc agcgcaatac
     6961 cttcggtaat cgagaagctg aacggcatca tcacagcggt aataaacgcc ggaacagatt
     7021 cagtaagatc ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaaa
     7081 tcagcgcgcc agctgcagcg tagcctggca ccattcccgc cagcggcgac agaaagataa
     7141 ccagcaggaa cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata
     7201 cgccggaaga ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca
     7261 cggaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaatttc cccttctcat
     7321 ctgccagacc ggctttatcg gtcacgccaa tcaacgtgcc ggaggagtca aacaggttga
     7381 ccagcatgaa agagaaaatc acccctgcca gcccgaggtt aaacgacccg gctaaatcta
     7441 catgacccac gacagtcatt acgctcggcg gcgcagaaac gatgccattg tagtggacat
     7501 cacccagcat ccagcccagc agcgttgtca ccacgataga aaccagcacc gctgcgtgaa
     7561 tgttgcgtga ggccagaata gcaatgatga agaagccgag gatccccagc agcacgctgt
     7621 gagaagtcag attaccgatg ctgaccagcg tttccgggtt agcgacaatc acacctgcgt
     7681 ttttcagccc catcatgcca atgaacagac cgataccgct ggtaatgccc acacgcagac
     7741 tcaccggaat gttggcaatc atccagtagc gaacgcggaa aatcgtcagt aacagcagac
     7801 caatcgcgcc ccagaagatt gcgcccatcc cgacttgcca cggcaagccc atcgcctgca
     7861 caacgacaaa agcgaagaac gcattcaggc ccatagcggg tgccagtgca actggcaggt
     7921 tagcaaacag ccccatcata atactgccga atgcagcaat cagacaggta gtgacgaaga
     7981 cggcgctggt atccatgcca gcaacgccaa gaatttgcgg gttaacaaaa acgatgtaaa
     8041 ccatcgtcag gaaggtggta aaaccggcga tcacttcggt ccgtgccgtc gtgccatgtt
     8101 cgcgcagttt aaacacgcgt tccagcatcc cctggccaga agcctgggtt gtgtgttgtt
     8161 gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatga
     8221 gacaatatgg acggctgaag ataacttttt tcattgtatt tgcttatacg gcaacgattg
     8281 cgtcatgcga attggcatga cgaaaacgtt aaactaacga taaaggaaag acatgtccca
     8341 gatagaagcg gtatttttcg actgcgacgg tacgctggtc gatagcgagg tcatttgctc
     8401 tcgcgcctat gtcacgatgt tcagggaatt tggcattcac gttgacctga aagagatttt
     8461 tacccgtttt aaaggcgtaa aactctacga aatcatcgat atcatcagta aagaacaggg
     8521 cgtgacgatg gtccaggccg atgccgagca cgtttaccgc gcagaagtag cacgtctgtt
     8581 tgataccgaa cttgaggaca tcgcaggagc gaacgaattg ctggcgagca ttaacgtgcc
     8641 gatgtgcgtc gtctctaacg gtccggtcag caaaatgcag cattcgcttg gtaagctcaa
     8701 catgctgcac tacttcccgg aaaaactgtt cagcggatat gacattcagc gttggaagcc
     8761 cgatccggca ttaatgttcc atgcggcaaa agcgatgaat gtgaacgttg aaaactgcat
     8821 tctggtcgat gactccagcg ctggcgctca atcagggata gatgccggaa tggaagtgtt
     8881 ttacttctgc gctgacccgc acaataagcc gatcgttcac ccgaaagtca ccacctttac
     8941 cgagctgtcg cagctgcctg cgctctggaa ggcacgcggc tggaatataa cgcgataatc
     9001 gcctccttac ttcaacactt acacatcagt cacgctggtt tgagatacag aacaccaggt
     9061 gtctgatgag taagcgtcta ttccctatga ataatttatg tcgttattcg tcctgtcatc
     9121 caccctctgt atggttaagc ctgggttata ttttgtacaa tatcgggata ccgttcgaac
     9181 caatccagaa tattacgcat cgcgtcactg atgtttttat cgtctttata ttgattctca
     9241 agtctattat ttcgaacaat agtaagctta gaacgtattt tatcatcaca atcgacaatt
     9301 ttcgcattca atgcatttac ttcatcctgc acttctatac tattccaatt gtcccttttg
     9361 ttatcaaata aagtcctttt atcctctaat gatttattac gacttatgat taactcttta
     9421 atttctcgca ataagtcatc gtaatcttta atggaagctc ttatttcctg atagtttttt
     9481 agtaaatcct cccagttaat tgcaaatgca ctggaaaaat agtaaaaatc gcgagcatac
     9541 ttattgagcc ttgagtcgta atctgtctta aacgatttta tatgttccag ttggtttgat
     9601 agtgctttaa tttgtttttc ttcaacacct tcaaaaatgg gtgcaagttc cttataatca
     9661 aatgttacat ttgtagcaga ttctttatga gactccttta tttgcttttg gacatattct
     9721 tttggatttt cagtaagtat tgttccatct ggcggcggaa tcattttatt tattatagat
     9781 tcaacaaact taatccggtc actttctatc atattgttaa tgatattttg tgcattttcc
     9841 tgcatatctt taaaaataaa aaactcaacc ctactattat caaaatgata tttttcctcg
     9901 tacgatttat tttcaacata aagtgttgat aaaaacgact ctaactctaa aaatgcgttt
     9961 ttatattttt ccttagtctt tgactcggaa ttataaaaat ttattacatc gatattgata
    10021 gcacttacct tttttaagaa atcaggtatt tgttctttac ttatagctcc gtccagatcc
    10081 atatacatgt atttttgcgc tgtaagacca tcttttgaat aatttgtatt tggatcaata
    10141 gcacagtctt tgcacctatc tccactgtaa taatagccta aatttgcccc aatcaaatta
    10201 ctatttttaa agaagacatt attgttctct gtatttttct tgctaaatga agaatcggtc
    10261 cctttcttta tactctctac gcatttcgaa aaattatcat ctaacttaag atttctcgat
    10321 cttctttcaa tg
//
LOCUS       DABSYT020000134        10170 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.134, whole genome shotgun sequence.
ACCESSION   DABSYT020000134 DABSYT020000000
VERSION     DABSYT020000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.134"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            72..275
                     /locus_tag="ID725_RS21515"
                     /old_locus_tag="pgaptmp_004303"
                     /pseudo
     CDS             72..275
                     /locus_tag="ID725_RS21515"
                     /old_locus_tag="pgaptmp_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            274..795
                     /gene="prfH"
                     /locus_tag="ID725_RS21520"
                     /old_locus_tag="pgaptmp_004304"
                     /pseudo
     CDS             274..795
                     /gene="prfH"
                     /locus_tag="ID725_RS21520"
                     /old_locus_tag="pgaptmp_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(852..2309)
                     /gene="pepD"
                     /locus_tag="ID725_RS21525"
                     /old_locus_tag="pgaptmp_004305"
     CDS             complement(852..2309)
                     /gene="pepD"
                     /locus_tag="ID725_RS21525"
                     /old_locus_tag="pgaptmp_004305"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGV7852775.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2570..3028
                     /gene="gpt"
                     /locus_tag="ID725_RS21530"
                     /old_locus_tag="pgaptmp_004306"
     CDS             2570..3028
                     /gene="gpt"
                     /locus_tag="ID725_RS21530"
                     /old_locus_tag="pgaptmp_004306"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGV7852776.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3120..4364
                     /gene="frsA"
                     /locus_tag="ID725_RS21535"
                     /old_locus_tag="pgaptmp_004307"
     CDS             3120..4364
                     /gene="frsA"
                     /locus_tag="ID725_RS21535"
                     /old_locus_tag="pgaptmp_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGV7852777.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4422..4823
                     /gene="crl"
                     /locus_tag="ID725_RS21540"
                     /old_locus_tag="pgaptmp_004308"
     CDS             4422..4823
                     /gene="crl"
                     /locus_tag="ID725_RS21540"
                     /old_locus_tag="pgaptmp_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGV7852778.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4862..5917)
                     /gene="phoE"
                     /locus_tag="ID725_RS21545"
                     /old_locus_tag="pgaptmp_004309"
     CDS             complement(4862..5917)
                     /gene="phoE"
                     /locus_tag="ID725_RS21545"
                     /old_locus_tag="pgaptmp_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGV7852779.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6205..7308
                     /gene="proB"
                     /locus_tag="ID725_RS21550"
                     /old_locus_tag="pgaptmp_004310"
     CDS             6205..7308
                     /gene="proB"
                     /locus_tag="ID725_RS21550"
                     /old_locus_tag="pgaptmp_004310"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGV7852780.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7320..8573
                     /gene="proA"
                     /locus_tag="ID725_RS21555"
                     /old_locus_tag="pgaptmp_004311"
     CDS             7320..8573
                     /gene="proA"
                     /locus_tag="ID725_RS21555"
                     /old_locus_tag="pgaptmp_004311"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGV7852781.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            8688..8763
                     /locus_tag="ID725_RS21560"
                     /old_locus_tag="pgaptmp_004312"
     tRNA            8688..8763
                     /locus_tag="ID725_RS21560"
                     /old_locus_tag="pgaptmp_004312"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8721..8723,aa:Thr,seq:cgt)
     gene            complement(8778..9698)
                     /locus_tag="ID725_RS21565"
                     /old_locus_tag="pgaptmp_004313"
                     /pseudo
     CDS             complement(8778..9698)
                     /locus_tag="ID725_RS21565"
                     /old_locus_tag="pgaptmp_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(9643..9888)
                     /locus_tag="ID725_RS21570"
                     /old_locus_tag="pgaptmp_004314"
     CDS             complement(9643..9888)
                     /locus_tag="ID725_RS21570"
                     /old_locus_tag="pgaptmp_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGV7852782.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTNRRIQ
                     MDAQTRRRERRAEKQAQWKADRILASTFRRNATKRHY"
BASE COUNT         2411 a         2513 c         2627 g         2619 t
ORIGIN      
        1 gtgactgggg tgaacgaacg cagtcgcagt acatgcaact tgaagtatga cgagtataaa
       61 tggaatggta catgggcaaa tatattcgtc ccttatccga tgcggtattt accatcgcat
      121 ctgatgacct gtggatcgag agtttagcga tccaacaatt acacaccacg gcaaatttac
      181 ccaacatgca gcgcgtagtg gggatgccag atttacaccc cggacgcggc tacccgattg
      241 gcgcagcgtt cttctctgtt ggtcgttttt acccgacaag acgtcgcggt aacggtgctg
      301 gaaacagaaa cgggccgcta ctctgacacg ctgcgttcgg cgctgatttc tctggatggc
      361 gacaacgcat gggcgttaag cgaaagttgg tgcggcacta ttcagtggat ttgtctgagt
      421 ccgtatcggc ctcatcatgg gcgcaaaaac tggtttctgg gcattgggcg ttttaccgct
      481 gatgagcagg aacaatcgga tgcaatccgt tatgagacgc tgcgtccgtc ggggccgggc
      541 ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca cgcatctggc aaccgggatt
      601 agcgtgaagg ttcagtcaga acgcagccag catgctaaca aacggctggc gcgattgctg
      661 attgcctgga agctggagca gcagcaacag gaaaatagcg cagtgctgaa atcgcagcgg
      721 cgaatgttcc atcaccagat tgaacgtggc aacccgcgac gaacgttcac agggatggct
      781 tttatcgaag gataatgaag gatgaaactg ccggaaggcg attaaacgcc atccggcagc
      841 aaatcaaata attacttcgc cggaatttct ttcagcagtt cagtcagcag tgtccagtaa
      901 tgacctacgc tttcgatgtg aacttgctca tccggagagt gtggaccggt gattgttggc
      961 ccgatagaaa ccatgtccat ttccggatac ggttttttga acagaccaca ttccaggccc
     1021 gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc gctgataggt ttcacgtacc
     1081 agatgcatca ccggagaatt agcgtccggc tgccagccag gatatgcgcc tttcgcttcg
     1141 gttttcgcgc cagccagttt acccagcgaa tccagcatgc tcaccacgta gtctttaccg
     1201 ctgtcgatca gagaacggat cagacagtga atttctacgt tattgtcagt catggtcacc
     1261 acaccgacgt tcagggaggt ttcgaccaca ccttttgcca cgtcggagtt gcggatcaca
     1321 ccgttcgggg tggcgttcag cagacgaata aaggtatcgc gagatttcgc aatcagggca
     1381 gctttatcgt tcgctacaga gtccagcaac aaggccagat ttttctcttt ctctgccagc
     1441 tcgtttttca ggatctcctg ataggtattc accagagatt tcagggcgtc gactttatca
     1501 gctgcgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag tgtgccgccg
     1561 ttgaaatcaa taagacgcag gtccagttct tctgcatgac ccgccaggaa gcgcaccagc
     1621 agtttgttgg cattacccag gccaacgtgg atttccccgc cggagtgacc gcctttcaga
     1681 ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg atctaaatgc
     1741 aggttggagg tgaagtcgat accccccgcg caacccatgt agatttcacc ttcttcttcg
     1801 gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc gaacgcaccg
     1861 tccataccgg cttcttcggt catggtcagc agcacttcca gcgggccgtg aaccacgttt
     1921 tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc cgttatccgc acccagcgtg
     1981 gtgccgcgcg ctttaaccca ttcgccatca atatatggct ggataggatc tttcgtgaag
     2041 tcatgcacgg tgtcgttatt tttctgcggc accatatcga ggtgggcctg caagacgacc
     2101 ggtttacgat tttccatacc tgcggtagca ggtttacgaa tcaggatatt acctacctga
     2161 tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc agcgagttgc
     2221 tcttcatgat aggacgggtg aggaatagaa cagattttgg caaaaatatc ccacagcggc
     2281 tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac
     2341 agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg cttgcgagat
     2401 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
     2461 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
     2521 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
     2581 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
     2641 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg taccgggtgc
     2701 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     2761 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     2821 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     2881 tccaaaagcg cactttgtca ccatctttgc aaaaccggct ggtcgtccgc tggttgatga
     2941 ttatgttgtt gatatcccgc aagatacctg gatcgaacag ccgtgggata tgggcgtcgt
     3001 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     3061 tctttttaac ttcaggcgag ttacaatagt ttccagtaag tattctggag gctgcatcca
     3121 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     3181 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
     3241 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     3301 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
     3361 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
     3421 gggccaccca ggcgatggtg tggcaacaaa aagcctgtgc ggaagaagat ccgcaactca
     3481 gtggtcgtca ctggctgcat gcggctacgt tgtacaacat tgccgcctat cctcatctga
     3541 aaggagatga cctggccgag caagcgcagg ctttgtcaaa ccgcgcctat gaagaggccg
     3601 ctcagcgtct accgggcacg atgcgacaga tggagtttac cgtacccggc ggtgcgccca
     3661 tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt cccgacggta ttaatgtgtg
     3721 gtggtctgga tgcgatgcag acggactatt acagcctgta tgaacgttat tttgcgccgc
     3781 gcggcattgc gatgctgact attgatatgc cgtcggtggg cttttcttca aaatggaagc
     3841 tcacccagga ctcctgcctg ttgcatcagc gcgtcttaaa ggctctgcct aacgtaccgt
     3901 gggtggatca cactcgcgtc gcggcctttg gtttccgttt cggcgctaac gttgccgtgc
     3961 gtctggcata tcttgaatcg ccgcgtctga aagcggttgc ctgtcttggt ccggtagttc
     4021 ataccctgtt gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc
     4081 tggcgagtcg tttggggatg catgatgctt ccgacgaagc gttgcgcgtg gagctgaatc
     4141 gctattcatt aaaagtgcaa ggattgctgg gacgtcgatg cccaacgcca atgttatcag
     4201 gctactggaa gaacgatccg ttcagcccgg aagaggactc acgcttaatc acctcatcat
     4261 ctgctgacgg taaattatta gagatcccat ttaacccggt gtatcggaat tttgacaaag
     4321 gtcttcagga aatcaccgac tggatcgaaa aacgcttgtg ttaaaaattt gctaaatttt
     4381 gccaatttgg taaaacagtt gcatcacaac aggagatagc aatgacgtta ccgagtggac
     4441 acccgaagag cagattgatc aaaaaattta ccgtactagg cccgtatatt cgtgaaggta
     4501 agtgcgaaga taatcgattc tttttcgatt gtctggctgt atgcgtcaac gtgaaaccgg
     4561 caccggaagt gcgtgagttc tggggctggt ggatggagct tgaagcgcag gaatcccgtt
     4621 ttacctacag ttaccagttt ggtctgttcg ataaagcagg cgactggaag agtgttccgg
     4681 taaaagatac tgaagtggtt gaacgactgg agcacaccct gcgtgagttt cacgagaagc
     4741 tgcgtgaact gctgacgacg ctgaatctga agttggaacc ggcggatgat tttcgtgacg
     4801 agccggtgaa gttaacggcg tgagtgaaat gtgccggatg catcacatcc ggcaataatc
     4861 attaaaactg ataggtcatg ccaaccgcga caatatcatc attattaata ttcaatttgt
     4921 tatcgctatc cagttggttg attttataat caacaaacgc tgacatattt ttgttgaaat
     4981 aatatgtagc cccgacatcg atataattga ccagatcttc atcaccgata ccttcaatat
     5041 ctttcccttt cgataagaca taacccagcg atggacgcag accaaagtca aactggtatt
     5101 gagcgaccgc ttcaaagttc tgtgtcttat tggcaaagcc gccagttatt ggcgtcattt
     5161 tgcgtgtttc agaataaaaa gttgccagat aaatattatt ggcatcgtat ttcagacctg
     5221 tagcccaagc ttctgcacgc ttgcctgtgc cacggctttg caggttctgc tcgttggtgc
     5281 gatctgagtt ggtataggcc ccactaatgg cgaaatcgct gccgccaaag tcatatgtca
     5341 atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc gcggttttcg tttttccctt
     5401 gatattgcag ggttaagttc aggccatcga taacgccgaa gaagtcggtg ttccgatacg
     5461 tcgccagacc gctggcgcgt ttggtcataa agttgtcggt ctgcgccgag gagtcgccac
     5521 caaattccgg gaacatatcg gtccaggctt ccacgtcata caacgcgccc aggttacggc
     5581 catagtcgaa agaacccaaa tctttatact tcaatccggc aaaagcgaga cgcgtttttt
     5641 gctgtgcagt atcactctcc gctttatttc cggcaaactc cgcttcccaa cgaccataac
     5701 cagtcagttg atcgttaatt tgtgtttcgc ctttaaaacc aaaacggata taactctggt
     5761 cgccatcttt actgtcgtta tcactcatat aatgcatggc tttaactttg ccatagacat
     5821 ccagtttatt accgtctttg ttatatattt ctgcggcctg tacggatgca gatgccacaa
     5881 tgcccatcac cactaatgcc agagtgctct ttttcatttt cattcctgat tttaattaac
     5941 gcgcgaatat tctgcgggag agccccgttg aaaacaggaa agtttttaac ctgagattgt
     6001 taaagatata ttacagatta atgatattct taaaatgtgg taatttatta aatctgtaat
     6061 aaaagcgtaa acaactgccg ctaggcttgc tgatcccgcg caacaaaacg ccatgctttg
     6121 ctcgcagatg gttggcaacc gacgacagtc ctgctaaaac gttcgtttga tatcattttt
     6181 cctaaaattg aatggcagag aatcatgagt gacagccaga cgctggtggt aaaactcggc
     6241 accagtgtgc taacaggcgg atcgcgccgc ctgaaccgtg cccatatcgt tgaacttgtt
     6301 cgccagtgcg cgcagttaca tgccgccggg catcggattg ttattgtgac gtcgggcgcg
     6361 atcgccgccg gacgtgagca cctgggttac ccggaactgc cagcgactat cgcctcgaaa
     6421 caactgctgg cggcggtagg gcagagtcga ctgattcaac tgtgggaaca gctgttttcg
     6481 atttatggca ttcacgtcgg gcaaatgctg ctgactcgtg ctgatatgga agaccgtgaa
     6541 cgcttcctga acgcccgcga caccctgcgt gcgttgctcg ataacaatat cgttccggta
     6601 atcaatgaga acgatgctgt cgctacggca gagattaaag tcggcgataa cgacaacctt
     6661 tctgcactgg cggcgattct ggcgggtgcc gataaactgt tgttactgac cgatcaaaaa
     6721 ggtttgtata ccgctgaccc gcgcagcaat ccgcaggcag aactgattaa agatgtttac
     6781 ggcattgatg acgcactgcg cgcgattgct ggtgacagcg tttcaggcct cggaactggc
     6841 ggcatgagta ccaaattgca ggccgctgac gtggcttgcc gtgcgggtat cgacaccatt
     6901 attgccgcgg gcagcaagcc gggcgttatt ggtgatgtga tggaaggcat ttccgtcggt
     6961 acgctgttcc atgcccaggc gactccgctt gaaaaccgta aacgctggat tttcggtgcg
     7021 ccgcctgcgg gtgaaatcac ggtagatgaa ggggcaaccg ccgccattct tgaacgcggc
     7081 agctccctgt tgccgaaagg cattaaaagc gtgactggca acttctcgcg tggtgaagtc
     7141 atccgcattt gtaacctcga aggtcgcgat atcgcccacg gcgtcagtcg ttacaacagc
     7201 gatgcattac gccgtattgc cggacaccac tcgcaagaaa ttgatgcaat actgggatat
     7261 gaatacggcc cggttgccgt tcaccgtgat gacatgatca cccgttaagg agcaggctga
     7321 tgctggaaca aatgggcatt gccgcgaagc aagcctcgta taaattagcg caactctcca
     7381 gccgcgaaaa aaatcgcgtg ctggaaaaaa tcgccgatga actggaagca caaagcgaaa
     7441 tcatcctcaa cgctaacgcc caggatgttg ctgacgcgcg agccaatggc cttagcgaag
     7501 cgatgcttga ccgtctggca ctgacgcccg cacggctgaa aggcattgcc gacgatgtac
     7561 gtcaggtgtg caacctcgcc gatccagtgg ggcaggtaat cgatggcggc gtactggaca
     7621 gcggcctgcg tcttgagcgt cgtcgcgtac cgctgggggt tattggcgtg atttatgaag
     7681 cgcgcccgaa cgtgacggtt gatgtcgctt cgctgtgcct gaaaaccggt aacgcggtga
     7741 tcctgcgtgg tggcaaagaa acctgtcgca ctaacgcggc aacggtggtg gtgattcagg
     7801 acgccctgaa atcctgtggc ttaccggcgg gtgccgtgca ggcgattgat aatcctgacc
     7861 gtgcgctggt cagtgaaatg ctgcgtatgg ataaatacat cgacatgctg atcccgcgcg
     7921 gcggggctgg tttgcataaa ctgtgccgcg agcagtcgac gatcccggtg atcacaggtg
     7981 gtataggcgt atgccatatt tatgttgatg aaagtgcaga gattgctgaa gccctgaaag
     8041 taatcgtcaa tgcgaaaact cagcgtccga gcacatgtaa tacggtagaa acgttgctgg
     8101 tgaataaaaa catcgcctat agcttcctgc ccgcattaag caaacaaatg gcggaaagcg
     8161 gcgtgacgtt acacgcagat gcatctgcgc tggcgcagtt gcagacaggc cctgcgaagg
     8221 tggtggcggt taaagccgaa gagtatgacg atgagtttct gtcattagat ttgaacgtca
     8281 aaatcgtcag cgatcttgac gatgccatcg cccatattcg tgaacacggc acgcaacact
     8341 ccgatgcgat cctgacccgc gatatgcgca acgcccagcg ttttgttaac gaagtggatt
     8401 cgtccgctgt ttacgttaac gcctctacgc gttttaccga cggcggccag tttggactgg
     8461 gtgcggaagt ggcggtaagc acacaaaaac tccacgcgcg tggcccaatg gggctggaag
     8521 cactgaccac ttacaagtgg atcggcattg gtgattacac cattcgtgcg taaataaaac
     8581 cgggtgatgc aaaagtagcc gtttgattca caaggccatt gacgcatcgc ccggttagtt
     8641 ttaaccttgt ccaccgtgat tcacgttcgt gaacatgtcc tttcagggcc gatatagctc
     8701 agttggtaga gcagcgcatt cgtaatgcga aggtcgtagg ttcgactcct attatcggca
     8761 ccatttaaat caataagtta cacatcatta gtaccttcct tattttttga ctgggacaaa
     8821 tttgggaccg atgggttcag gatcgagtct atttgccgtg cgtgttcggt aaggtgatta
     8881 ggtgcaaggt gagcatatcg acgaaccatt tcgatagact cccagcctcc catttcctgt
     8941 aacactgaca acgggactcc ggcttgaacc agccaacttg cccaggtgtg tctcaagtcg
     9001 tgaaatctga aatcatcaat accagcccgt ctcagcgccg ctttccaggc tgtgtttgcg
     9061 tcataccgca tcttccttac tgttggcgct ttcgttccgt ctggtttggt acagctttcc
     9121 ttgtacacaa atacccaacg gtgatgatta ccgatttgtt ttttcaatac gcgacatgca
     9181 gtatcattca gcgcaacgcc aattgcgcgg tttgatttac tctcttccgg gtttatccat
     9241 gccacccggc gctgcatatc tatttgttgc cattcaaggt tgatgatgtt cgagcgtctt
     9301 aagcctgttg ccagtgcaaa ttcaacaaca gactttaatg gctccggaca ttcatcaatc
     9361 agcctttgtg cttcatgggg ctccagccag cggatccgtt tattctttgg ttgaggcact
     9421 ttaataattg gtgccttatc gagcattttc cattcacgct ctgcggctct tagcagggcc
     9481 tttataaatg aaagatgcgt agccttcgtt gcaacggacg ctggttttgg cgtgtattct
     9541 ggaacaggtt tccctttttt tctgcatgct tctgccctga gtttccagtt ttcctcatga
     9601 cgccggttcg tcattttctg cattgctgaa taaatttttg attcagtaat gtctcttagt
     9661 tgcattcctg cgaaatgttg aagccagaat ccgatctgct ttccattgag cctgtttctc
     9721 tgcgcgacgt tcgcggcggc gtgtttgtgc atccatctgg attctcctgt tagttagctt
     9781 tggttgtgtg tgggagtcgt agtcctgaac gaaaacaccc cgcaatggca cattggctgc
     9841 taatccggat tcgcacttcc ggccaatgct tcgtttcgta tcacacacac caaagccttc
     9901 tgctttgaat gctgcccttc ttcagggctt aatttttaag agcatcacct tcatggtggt
     9961 tagtgcgtcc tgctgatgtg cttagtatca ccgctagtgg tatttgtgtc aacaccgcca
    10021 gagataattt atcaccgcag atggttatct gtatgttttt tatatagatt tattttttgc
    10081 agggttgtgt ggcttgggag gtgatcgaga gatcagaatt gcgttgttta gtgagttgca
    10141 cctattaatt ttaaaataaa tacaattggt
//
LOCUS       DABSYT020000135        10168 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.135, whole genome shotgun sequence.
ACCESSION   DABSYT020000135 DABSYT020000000
VERSION     DABSYT020000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.135"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            172..324
                     /gene="hokA"
                     /locus_tag="ID725_RS21575"
                     /old_locus_tag="pgaptmp_004315"
     CDS             172..324
                     /gene="hokA"
                     /locus_tag="ID725_RS21575"
                     /old_locus_tag="pgaptmp_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGV7852783.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(301..372)
                     /locus_tag="ID725_RS21580"
                     /old_locus_tag="pgaptmp_004316"
     CDS             complement(301..372)
                     /locus_tag="ID725_RS21580"
                     /old_locus_tag="pgaptmp_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852784.1"
                     /translation="MRNAVRKAGITFRRRLLLFQIAG"
     gene            complement(512..724)
                     /gene="cspA"
                     /locus_tag="ID725_RS21585"
                     /old_locus_tag="pgaptmp_004317"
     CDS             complement(512..724)
                     /gene="cspA"
                     /locus_tag="ID725_RS21585"
                     /old_locus_tag="pgaptmp_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGV7852785.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1005..1295)
                     /gene="yiaG"
                     /locus_tag="ID725_RS21590"
                     /old_locus_tag="pgaptmp_004318"
     CDS             complement(1005..1295)
                     /gene="yiaG"
                     /locus_tag="ID725_RS21590"
                     /old_locus_tag="pgaptmp_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGV7852786.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1729..2439
                     /gene="yiaF"
                     /locus_tag="ID725_RS21595"
                     /old_locus_tag="pgaptmp_004319"
     CDS             1729..2439
                     /gene="yiaF"
                     /locus_tag="ID725_RS21595"
                     /old_locus_tag="pgaptmp_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGV7852787.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2489..3463)
                     /gene="ghrB"
                     /locus_tag="ID725_RS21600"
                     /old_locus_tag="pgaptmp_004320"
     CDS             complement(2489..3463)
                     /gene="ghrB"
                     /locus_tag="ID725_RS21600"
                     /old_locus_tag="pgaptmp_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGV7852788.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3567..4226)
                     /gene="yiaD"
                     /locus_tag="ID725_RS21605"
                     /old_locus_tag="pgaptmp_004321"
     CDS             complement(3567..4226)
                     /gene="yiaD"
                     /locus_tag="ID725_RS21605"
                     /old_locus_tag="pgaptmp_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGV7852789.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4379..6712
                     /gene="bisC"
                     /locus_tag="ID725_RS21610"
                     /old_locus_tag="pgaptmp_004322"
     CDS             4379..6712
                     /gene="bisC"
                     /locus_tag="ID725_RS21610"
                     /old_locus_tag="pgaptmp_004322"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGV7852790.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6681..7121)
                     /gene="yiaC"
                     /locus_tag="ID725_RS21615"
                     /old_locus_tag="pgaptmp_004323"
     CDS             complement(6681..7121)
                     /gene="yiaC"
                     /locus_tag="ID725_RS21615"
                     /old_locus_tag="pgaptmp_004323"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGV7852791.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7118..7681)
                     /gene="tag"
                     /locus_tag="ID725_RS21620"
                     /old_locus_tag="pgaptmp_004324"
     CDS             complement(7118..7681)
                     /gene="tag"
                     /locus_tag="ID725_RS21620"
                     /old_locus_tag="pgaptmp_004324"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGV7852792.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQTGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7839..8537
                     /gene="yhjY"
                     /locus_tag="ID725_RS21625"
                     /old_locus_tag="pgaptmp_004325"
     CDS             7839..8537
                     /gene="yhjY"
                     /locus_tag="ID725_RS21625"
                     /old_locus_tag="pgaptmp_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGV7852793.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8766..9968
                     /gene="yhjX"
                     /locus_tag="ID725_RS21630"
                     /old_locus_tag="pgaptmp_004326"
     CDS             8766..9968
                     /gene="yhjX"
                     /locus_tag="ID725_RS21630"
                     /old_locus_tag="pgaptmp_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7852794.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
     gene            complement(9976..>10168)
                     /locus_tag="ID725_RS21635"
                     /old_locus_tag="pgaptmp_004327"
     CDS             complement(9976..>10168)
                     /locus_tag="ID725_RS21635"
                     /old_locus_tag="pgaptmp_004327"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852795.1"
                     /translation="KKIIALHFHIKSYRLFLGINKYKIPIKQNKNIHHANNKIKYFHF
                     YSLKCNKKGGFPPPFVQNL"
BASE COUNT         2435 a         2448 c         2600 g         2685 t
ORIGIN      
        1 cttattaagc tagaatagcc acgggtgctt gagactgttt gtctcaggta ttcaccgaaa
       61 ggcagacaga gaaaagcccc acctgactat aaatcaaagt gaggcccctc tatgtataga
      121 caccataagg ttagcctctt actcgttgga aatcaacaca gggggctggg aatgccgcag
      181 aaatatagat tgctttcttt aatagtgatt tgtttcacgc ttttattttt cacctggatg
      241 ataagagatt cactgtgtga attgcatatt aaacagggga gttatgagct ggcggcattt
      301 ttagcctgca atttgaaaga gtaagagtct tcggcggaaa gttattcccg cctttcttac
      361 ggcgttgcgc attctcattg cacccaaatt tatttttcac aaaaataata ctagatttta
      421 ttacgcgatc gattatttat ttcctgaaaa acaaataaaa aaatccccgc caaatggcag
      481 ggatcttaga ttctgtgctt ttaagcagag attacaggct ggttacgttg ccagctgccg
      541 ggcctttagc gccgctttcg atggtgaagg acactttctg accttcgtcc agagatttgt
      601 aaccatcgtt ctggatagca gagaagtgta cgaacacgtc tttagagcca tcgtcaggag
      661 tgatgaagcc gaagcctttg tcagcgttga accattttac gataccagtc attttaccgg
      721 acatagtgta ttacctttaa taattaagtg tgcctttcgg cgatatggcg tgctttacag
      781 attttgaagc gttaaaggaa tgtgcactac gaggggtatc aacgataact cttgaaggga
      841 cttgccttac tacactgctt taatggtctg tacgtcaaac cgttgatgtg cattaagcca
      901 cgcattggcg ggtgatgcaa caattatttt tcatatttat gattaatcgg ttggaaaacg
      961 gtcctgtcat caggaccgta aacagcaata aagtggataa aagtctattc catcaactgc
     1021 ttacttaatg ccgggttggc ttgaatcaaa cgcatcaatt ttagttcggc acttgaaggc
     1081 ttcacgcgtc tggattccca ttcctttacc atggcgactg atacgcccaa aacccgggcg
     1141 aaatcatcga tttttaatcc tgtccctttt cgtaactgct caatttcggt acaggacgtt
     1201 gttctgtgcg tcagggtaat tttctgcgtt tcatctttaa aaacaatctg ttccaggctg
     1261 ctcaacagct catgcattgg atctttatat tccattgaaa actcctcaaa tcacactgcg
     1321 ggatcgtgaa ttgcatcgaa gctcattaag aatagtcggg aaaagcagac acagcggggt
     1381 gaagtcaggg catgctctgg ctatgattcg attcagccag tcatttagcg tgacttatag
     1441 ccacctaacg atctgataaa tgccgatata tctctaacca ctcctggttt gtaaagattt
     1501 cgaaaatcta accttctgcg aaggcgtaaa tctctggcta caaaaaaggc aacggcaata
     1561 tcatttttta acaatgaaac aacaaattaa ccttgtttca ttttctccat gattttacca
     1621 ggaagattac gtagaaaggg tatcctgcaa gcctgtcctg gactatcctt gtcacgtcag
     1681 acacgcgtgt gtcgttgtgc gctttttttg ggtgaaagga gtaagaaaat ggcgacagga
     1741 aagtcctgct ctcgctggtt tgcgcctctt gcggcgttat taatggtagt tagcctgagt
     1801 gggtgttttg ataaagaagg cgatcagcgt aaagcgttta tcgacttcct gcagaataca
     1861 gtgatgcgta gcggtgaacg tctaccaacc ctgactgccg atcagaaaaa acagtttggt
     1921 ccttttgtct ctgattacgc gattctgtat ggttattctc agcaggtgaa tcaggcgatg
     1981 gattccggtc tgcgcccggt tgtagacagc gttaacgcaa ttcgcgtacc gcaggattac
     2041 gttacgcaaa gcggtccgct gcgtgaaatg aatggttcgc tgggtgtgct ggcgcaacaa
     2101 ctgcaaaatg cgaagttgca ggccgatgct gcgcactctg cgctaaaaca gagtgatgac
     2161 ctgaaaccgg tctttgatca ggcattcacc aaagtggtaa cgacgccagc tgatgcattg
     2221 caaccgttga ttccggcggc acaaactttc acgcaacaac tggtgatggt tggagactac
     2281 attgcccaac aaggtactca ggtgagcttt gtagcaaatg gcatccagtt cccgacttca
     2341 cagcaggcaa gtgaatataa caaactgatt gcgccattac cggcacaaca tcaggcgttt
     2401 aatcaggcct ggactacagc agttactgca acccaataaa gagtaaaagc ccgagcgatt
     2461 atcgggcttt actccacgca gtcgcggctt agtccgcgac gtgcggattc acacagttct
     2521 tctcaacctt tccttgtaac gcatcaatca aattatccac ggcacaggcg gccatgccat
     2581 aacgcgtctc atgggtggca gatccaatat gcggtactgc gacgacgttg gccattgaga
     2641 gcaacggcga atctacggaa agtggctctt gttcgaagac atccagcccg gcggcgtgaa
     2701 tttccccttt ctgcaatgct gcgatcagtg cattttcgtc aaccaccggg ccacgtccgg
     2761 cattaatgaa aatggcggag gatttcattt tggcgaattg ttctgcgcca aacagatgat
     2821 gcgtctcatc agttaacggc aggatcaggc aaacgaaatc tgactcttgt aacagtgtat
     2881 ccaaatcgca gtagcgggcg ttgaagcgtt cttctgcttc tttatggtgg cggcgcgcgt
     2941 tatagaggat gggcatgttg aagccaaagt gcgcacgttg tgccagcgcc ataccgatcc
     3001 gtcccatccc gacaatgccc agtgttttat ggtgaacgtc agtgccgtac cagtccgggc
     3061 ctatgctcgc ggtccattcg cctgctttta cccgttctgc tacctccaca acccgacgag
     3121 cggtagacaa caccagcgcc atcagcgtat cggcgacggt ttctgttaag acggttggcg
     3181 tgtgcatcag cagaattttt cgggcggtaa gcgcatcgac atcaaaattg tcatagccga
     3241 cggagatcgt tgatgtggca cgcagtttcg gcattttttc cagcaatgcg gcatcaacat
     3301 tctcgtttga acccagtaaa ccttcagctt cggcaaaaat tgctgcattt tgttcgacgg
     3361 tttgtgggct gaggtttgcc acctggtgaa cggtgaaatg ctcttgcagg cgttgcagta
     3421 aatcatcagg taaggctttg tagaggataa cggacggctt catgcttctc tccattcact
     3481 gataaggcga tcaccttagc ctgaaatatg atgaaggaaa actgaagaat gccaggtgat
     3541 ttctgcatca cctggcatga aagggattac agcgggctta aggtaatttc tacacggcgg
     3601 ttttgcgcct taccttctgc ggtgctgttg ctggcgattg ggttagccgg gccaaggccc
     3661 tgagtacgga tgcggctggc gtccacgccc tgggtgatca acgcgctggc aacggaatcc
     3721 gcacgctgct gggagagacg catgttcaga tcgtgaccac ccgtgctgtc agtataacca
     3781 atcacgttaa ccgccgtttt cggatactct ttcagcacca ttgccacgcc ggtcagggtg
     3841 ttagcgcccg ccggtttcag ggttgcgctg ctgctgtcga aggtcacatt gttcggcata
     3901 ttgaggataa tgttatcccc gctgcgggtt acgctaacac cagtgccgcg cattttgtca
     3961 cgcagcttcg cttcctgcac atccatgtaa taacccacgc cgccgcccag agctgcgcct
     4021 gctgctgcgc caatcagcgc gcctttaccg cgatctttct tcgaagaaga gagcgcacca
     4081 atacccgcgc ccacgagaga gcccagacct gcgccgatag cagatttacc tgcttcgcgt
     4141 tcgccggtgt aagggttagt tgtgcagcca gataccgcca gagcgccact cactacggcg
     4201 gcaataagat aaacacgttt cttcattgtt aatccttaat aaccttttta ttctttgcca
     4261 cgggttccgt ggcgggagat tatgccgcgt gaacatgaag attattcctg ggaatactcg
     4321 gaaatttgta agtaatattt aactgctcaa tacatctaac ctttcaggag tcttcggttt
     4381 gaccaactca tcctcacgat attccgttct gactgccgcc cattgggggc ccatgctggt
     4441 tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg ttagccacag gaatggaaaa
     4501 ctccttgcag agcgcggttc gtgaccaggt tcacagcaat acgcgggtac gatttccaat
     4561 ggtgcgcaaa ggctttctcg cgtcaccgga aaacccgcaa ggcattcgtg ggcaggatga
     4621 atttgttcgc gtgagttggg atgaggcgct ggatcttatt caccaacaac ataaacgcat
     4681 tcgtgaggct tatggtccgg catcgatttt tgctggttcc tacggctggc gttcaaacgg
     4741 cgtgctgcat aaggcctcga cattattaca acgctatatg gcgctggcag gcggttatac
     4801 cgggcatctg ggggattatt cgaccggcgc ggcacaggcg atcatgccgt atgtcgtggg
     4861 tggtagtgaa gtttatcaac agcagaccag ttggccgctg gtgctggaac atagcgatgt
     4921 cgtggtgctg tggagtgcta acccactcaa tacgctgaaa attgcgtgga atgcatccga
     4981 tgagcagggg ctttcttact tttctgcact gcgtgacagc gggaaaaagc tgatctgcat
     5041 tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat aaaatggagt gggtggcacc
     5101 gcacatgggc actgatgttg cgctgatgct ggggatcgcc catacgctgg tggaaaatgg
     5161 ttggcacgac gaagcgtttc tggcgcgttg caccaccggt tatgccgtct tcgcctctta
     5221 tttgctgggc gagagtgacg gaatagcgaa aaccgccgaa tgggcagcag agatttgtgg
     5281 tgttaacgca gagaaaatcc gcgagctggc ggctattttc caccaaaata ccaccatgct
     5341 gatggctggc tggggaatgc agcgccaaca gtttggtgag caaaaacact ggatgatcgt
     5401 cacgctggca gcaatgttgg ggcaaatcgg cacacccggc ggcggttttg gtctttctta
     5461 ccattttgcc aatggtggta accccacgcg gcgcgctgcg gtactctctt ccatgcaggg
     5521 cagcttgccg ggtggctgcg atgcggtaga taaaatccct gtcgcccgca ttgttgaagc
     5581 actggaaaac cccggtggcg catatcaaca caacggtatg gaccgacatt tcccggatat
     5641 tcgttttatc tggtgggcgg gcggtgccaa ctttactcat catcaggata ccaatcgcct
     5701 gatccgtgcc tggcaaaaac cggagctggt ggtgatctct gaatgcttct ggacggcggc
     5761 ggcaaaacac gcggatatcg ttctgcctgc gactacctct tttgagcgta atgatctcac
     5821 catgaccggt gattacagta atcagcatct ggtgccgatg aagcaagtgg tgccgccacg
     5881 ctatgaagcg cgtaatgatt ttgatgtttt tgccgagtta agtgaacgct gggagaaggg
     5941 cggttatgca cgttttacgg aaggaaaaag tgagctgcaa tggctggaaa cgttttataa
     6001 cgttgcccgg cagcgcgggg caagccagca ggttgaattg ccgccattta ctgagttctg
     6061 gcaagccaac cagttaattg agatgccgga aaacccggac agcgagcggt ttattcgctt
     6121 cgctgatttt tgccgcgatc cgcaggcgca tccattaaaa accgccagcg gtaagattga
     6181 aatcttctcg cagcgtattg ccgattacgc ttatccggat tgcccggggc atccaatgtg
     6241 gctggtgccg gacgaatggc agggcaatgc cgaaccggaa cagttacagg tactttctgc
     6301 tcatccggcg catcgtctgc acagccagct gaattacagt tctctgcgcg aattgtacgc
     6361 ggtggcaaac cgtgagcctg tcaccattca tcctgacgat gcccaggcgc gcggcataca
     6421 agatggcgat actgttcggc tgtggaacgc acgcgggcaa attcttgccg gagcggtcat
     6481 tagcgaggga attaaacctg gcgtgatttg cattcacgaa ggggcatggc cggatctgga
     6541 tttaaccgct gacggtattt gtaaaaacgg cgcagtgaac gtgctgacca aagatctccc
     6601 cagctcgcgg ctggggaatg gctgtgcggg taatacggcg ctggcatggc tagaaaaata
     6661 caacggtccg gaactgacac ttacagcgtt tgaaccaccg gccagctcat aatccaggtg
     6721 ggtagttggg tttcatcctg ccatgcgcaa tcgacaatgt gaaaaccctg cgcctggtaa
     6781 aaatctatcg ccggttgatt tttttgataa acctccagca tcagatgggg atagcgctgc
     6841 tgcacatact gcatcagcgc cttaccaata ccgcgtctga cggccttcgg tgcgacaaac
     6901 attgctgcca gaaatcggcc ttccataatg ctgacaaaac cgagaagctt accgtcttct
     6961 tcccagaccc agtttttcgc attggcaaga tacgcatccc gcaccagcgg aatgcactcg
     7021 cgccagtaat tcacttttat aaagggatgc ccccaggttg tactttccag ccacagttcg
     7081 aggatcgcgg ggagttctga acgttgcgct tcccgaatca tggtttattt cccggatagc
     7141 aacagcagcc aaccacatga tcattcacca gcccacatgc ctgcataaag gagtaacaga
     7201 ttgtggtacc gacaaactta aaaccacgtt ttttcagtgc cttagatagg gcgtcggagg
     7261 cgggcgtaga tgtggggatt tcgctcaacg ttgtggcttg tgtcacctgt ggctgatgat
     7321 ttacaaacga ccagacaaag tcggcaaacg gttcgccgtt ctgttccatt tgcaggtacg
     7381 cccgcgcatt accaataatt gcctgaattt tcccgcgatg gcggataatc ccggcgtcct
     7441 gtaccagtct ttcgacatcc tcttcctgca ttgcggcaac cttcaccgga tcgaactgat
     7501 gaaagcaggc gcgatagttt tcgcgttttt tgaggacggt gatccacgat aacccagtct
     7561 gctgcccttc caggcagatc atttcgaaca gttttttcct gtcagtttca ggcacgcccc
     7621 actcattatc atggtaggca atataaagcg ggtcctgact cacccagccg caacgttcca
     7681 tactttccct cgctatgtac ccgatcgtaa aaatttcagc cgcttttctt gacgcatcgg
     7741 tgaaaaagtc aatatttaat acagggctac tatgcccgat atccttcata caatgacaat
     7801 aatattgccg aattcggctc ttccctggag tcgtgttaat gatcataaaa aaaagcggtg
     7861 gtcgctggca gctaagcctg ctggcgagtg tggtaatcag tgcctttttt ctcaacacag
     7921 cttacgcctg gcaacaagaa tatatcgttg atacgcaacc cgggcattcc acagagcgtt
     7981 acacctggga tagtgatcat caacctgatt acaacgatat tttgtcgcaa cgtattcaaa
     8041 gtagccaaag ggcgctggga ctggaagtca atctggcgga agaaacccct gtggatgtga
     8101 ccagcagtat gagtatgggc tggaattttc ctttatatga acaggttaca actggcccgg
     8161 tcgcggcatt acattacgat ggcacaacca cctcgatgta taacgagttt ggcgacagta
     8221 ctaccacgct gaccgatccg ttatggcatg ccagcgtgag taccttaggc tggcgtgttg
     8281 actcccggct tggcgatctc cgaccctggg cgcaaatcag ctataaccag caatttggcg
     8341 agaatatctg gaaggcgcaa tcaggcctga gccggatgac ggcgacaaac cagaacggca
     8401 actggctgga tgtcaccgta ggcgctgata tgttgctcaa tcaaaatatt gccgcctatg
     8461 ccgcgctatc tcaggcagaa aataccacta acaatagcga ttatctgtat acgatggggg
     8521 ttagtgccag attttaacgt aacaatcaca attgaaacca ttgaataaca atagttgtgg
     8581 cgatagtggg tgctaactta ccaaataata aatttggtga ataattgtcg cgtcattcat
     8641 tcctgaacta aggcatttca ttccgttctg atggcatttc atgccgtttt tccccaggca
     8701 taaagtgcac ttcgttatgg ttgtcggcag agatttttcc tttttattac tgcaggaata
     8761 ctgccatgac accttcaaat tatcagcgta cccgctggtt gacactcatc ggtactatca
     8821 ttacccagtt tgcgctgggg tcggtttata cctggagcct gtttaacggc gcgctttccg
     8881 ccaagctgga tgcgccggta agccaggtcg ctttctcttt cggcttgtta agtctggggc
     8941 tggcaatatc gtcttctgtt gcgggcaaat tgcaggaacg ttttggtgtt aaacgcgtca
     9001 ccatcgcttc cggcattttg ctgggattag ggttcttcct gacagcgcat tctgacaacc
     9061 tgatgatgct gtggttaagc gccggtgtgc tggtcggtct ggcggatggc gcgggttacc
     9121 tgctgacgct ctctaactgc gtgaaatggt tcccggagcg taaaggtctg atctccgcgt
     9181 tcgctatcgg ttcttatggt ctgggtagcc tgggtttcaa atttatcgac acgcagctgc
     9241 tggaaacggt cggtctggaa aaaacctttg tgatttgggg agcgattgcg ctggtgatga
     9301 ttgttttcgg cgcaacgtta atgaaagacg caccaaaaca ggaagtgaaa accagcaatg
     9361 gtgtggtgga gaaagattac acgctggcag agtcgatgcg taaaccgcag tactggatgt
     9421 tagcggtaat gttcctgact gcctgcatga gcggcctgta cgtgattggg gtagcgaaag
     9481 atatcgccca aagtctggca caccttgatg tggtttctgc agccaatgca gtcactgtta
     9541 tttccatcgc caacctttca ggtcgtctgg tgctgggcat tctgtctgac aaaatcgccc
     9601 gtatccgtgt tatcaccatt ggtcaggtaa tttcgctggt gggtatggcg gccctgctgt
     9661 ttgcaccatt gaatgcagtg acgttctttg cagcgattgc ctgcgtggca tttaactttg
     9721 gcggcactat taccgtcttt ccgtcactgg tcagtgagtt cttcggcctc aataacctgg
     9781 cgaaaaacta cggtgtgatt tacctcggtt tcggtatcgg tagcatttgt ggttcgatta
     9841 tcgcctcact gtttggcggc ttctatgtga agttctacgt catctttgct ctgctgattc
     9901 tgtcattggc gctttctacg acgattcgtc agccagagca gaaagtattg cgtgaggcgc
     9961 atatttagcc agaaactaca ggttttgaac gaaaggggga ggaaatcctc cttttttatt
    10021 gcattttaaa gaataaaaat gaaaatattt tatcttatta tttgcgtggt gaatgttttt
    10081 gttttgttta attggtattt tatatttatt tattcctaaa aataaacgat agctttttat
    10141 gtgaaaatgt aatgcaatga ttttttta
//
LOCUS       DABSYT020000136        10119 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.136, whole genome shotgun sequence.
ACCESSION   DABSYT020000136 DABSYT020000000
VERSION     DABSYT020000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.136"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1265)
                     /locus_tag="ID725_RS21640"
                     /old_locus_tag="pgaptmp_004328"
     CDS             complement(<1..1265)
                     /locus_tag="ID725_RS21640"
                     /old_locus_tag="pgaptmp_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGV7852796.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKNVPGVGALFGELAEENRV"
     gene            complement(1315..1617)
                     /locus_tag="ID725_RS21645"
                     /old_locus_tag="pgaptmp_004329"
                     /pseudo
     CDS             complement(1315..1617)
                     /locus_tag="ID725_RS21645"
                     /old_locus_tag="pgaptmp_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(1644..2066)
                     /locus_tag="ID725_RS21650"
                     /old_locus_tag="pgaptmp_004330"
     CDS             complement(1644..2066)
                     /locus_tag="ID725_RS21650"
                     /old_locus_tag="pgaptmp_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGV7852797.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKTEATEPVTSKKHSKAS"
     gene            complement(2080..2832)
                     /locus_tag="ID725_RS21655"
                     /old_locus_tag="pgaptmp_004331"
     CDS             complement(2080..2832)
                     /locus_tag="ID725_RS21655"
                     /old_locus_tag="pgaptmp_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7852798.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(2840..3238)
                     /gene="gpU"
                     /locus_tag="ID725_RS21660"
                     /old_locus_tag="pgaptmp_004332"
     CDS             complement(2840..3238)
                     /gene="gpU"
                     /locus_tag="ID725_RS21660"
                     /old_locus_tag="pgaptmp_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HGV7852799.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            complement(3251..3874)
                     /locus_tag="ID725_RS21665"
                     /old_locus_tag="pgaptmp_004333"
     CDS             complement(3251..3874)
                     /locus_tag="ID725_RS21665"
                     /old_locus_tag="pgaptmp_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7852800.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(3877..4158)
                     /locus_tag="ID725_RS21670"
                     /old_locus_tag="pgaptmp_004334"
     CDS             complement(3877..4158)
                     /locus_tag="ID725_RS21670"
                     /old_locus_tag="pgaptmp_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGV7852801.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(4151..4477)
                     /locus_tag="ID725_RS21675"
                     /old_locus_tag="pgaptmp_004335"
     CDS             complement(4151..4477)
                     /locus_tag="ID725_RS21675"
                     /old_locus_tag="pgaptmp_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HGV7852802.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(4565..6589)
                     /locus_tag="ID725_RS21680"
                     /old_locus_tag="pgaptmp_004336"
     CDS             complement(4565..6589)
                     /locus_tag="ID725_RS21680"
                     /old_locus_tag="pgaptmp_004336"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGV7852803.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(6534..8036)
                     /locus_tag="ID725_RS21685"
                     /old_locus_tag="pgaptmp_004337"
     CDS             complement(6534..8036)
                     /locus_tag="ID725_RS21685"
                     /old_locus_tag="pgaptmp_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGV7852804.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(8036..8248)
                     /locus_tag="ID725_RS21690"
                     /old_locus_tag="pgaptmp_004338"
     CDS             complement(8036..8248)
                     /locus_tag="ID725_RS21690"
                     /old_locus_tag="pgaptmp_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852805.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVIFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(8245..9369)
                     /locus_tag="ID725_RS21695"
                     /old_locus_tag="pgaptmp_004339"
                     /pseudo
     CDS             complement(8245..9369)
                     /locus_tag="ID725_RS21695"
                     /old_locus_tag="pgaptmp_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
     gene            complement(9741..10022)
                     /locus_tag="ID725_RS21700"
                     /old_locus_tag="pgaptmp_004340"
     CDS             complement(9741..10022)
                     /locus_tag="ID725_RS21700"
                     /old_locus_tag="pgaptmp_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852806.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQATQPDSMTADNYVRVVVEVMR
                     RDGIQLNDVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKTPERLRR"
BASE COUNT         2178 a         3173 c         2360 g         2408 t
ORIGIN      
        1 acgcggttct cttctgccag ttcaccaaac aacgcaccaa cgccgggcac attctttgtc
       61 gtttcccagg ctgactgaat aaattcagcc agcgccagat ccccctgcac aagcagcagc
      121 ttcacctgtt caacggttcc tgccaccacg tcagtgatca gactgagtgc ccccagtgta
      181 tgatcaccta tccatgccca tgcgtcagaa gtccaggttt taacatcgtc ccagattttt
      241 tccaccggcg tggccgcttt atcaagttgc tccagacgtg cattcatgac atccgcaaac
      301 agggacatcg cctccgtcac cgcagcctgt ttacctttcg tgcgctcaag ctcatcaata
      361 tggcgtaact gggaaacgct caggaagtta tactgctgat tcagggaggc cagcgccttc
      421 accggatctg ctgcaatccc ttcaaaggct ttttccacct tcccggcatc gtcccccacc
      481 gtctgcagcc atctctgaga ggtttccccc atgatccgta gctgcccggc ggtatatttc
      541 ccgctttctg ccagacgggc cagattttct gccgcctgtc tgataccacc accggcttca
      601 tcgctgatca ccccggccat tttccacaat tctgccgttg tggtggcagc agcccctccg
      661 gtcagggtca gtgaacgcaa taaggcccgg tcagcctgct ctgcctgcca ggcggcggca
      721 gcaagcgcgg ccagtacggc aaccccgcca cctgccgcca cacgggccac cgacataaat
      781 cgtcccagct caccggcatt ccgggcattt tcagccagtg catttgccgt atctgacagc
      841 gactcctctg atgattcaga ggcatccctg atcccgagaa gttcctcctt cagcagggta
      901 agcaggctga gcggtccacc gaatgaatcg ctgatctgcc ccccctgctg cagcatgata
      961 aggaagggat tctgaccacc ggcaagctga gtgacaatat ccgtgaactg tgcgggcagt
     1021 gtgcgcatgg cagccttata ctgtccgact gatatcccgg ctttttgtgc agccagcgcc
     1081 tgtcggctca gactctgctc agcagcatca gcctgttttc tgaaagactg actgactttc
     1141 ccggacatca aatcagcaag cgcactggtt tcacccagct ctttttttac ccgcgctgcc
     1201 tcttcagaaa aacgggcaga atccagtgtc agcacggctg tcagatcggc aaaattacct
     1261 gccatcgtgg tcacctcctg aaatgtcctc tgataccatc aataactgtc acaacctcct
     1321 tccctcctcc ccgaaacgga ctccaccggc gaggcccgcc gccttctgca tcagtacatc
     1381 attttcgtcc ggcgtctccg cctgtccttc ctctgcctcc ggagcgaaca ggctgaaatc
     1441 cgccggatgc atatccggat cgcaaaaaaa caggctgagt acggcgtaca tcagcccgga
     1501 aaaatgcata tccagctggg tatcctgaaa ataatgcgtg cagtaaaaac gtcgccagtc
     1561 ggcatattcg gtggatgtca tcccggcaag catggcgcgc cagtcggctc tctccatctc
     1621 acgcgccagt ttcaggacaa agttcagctc gccttcgaat gcttttttga tgttaccggc
     1681 tcagtcgctt ctgttttccc ggttggttca ggatcggcat cgtgccggtt atccagcata
     1741 cctgaaagat aaagcacccg gttcgttgcc tgattcagtg catcagcagg ccatcccagc
     1801 atcacttcac ggcggatctg ctgcatctct gtctccggag aggccagagt gcctttgagg
     1861 gaatgggaat gccatagcga catcgccaca agcagggatg ccgtttccag atatcgctgg
     1921 ttaatgtgca cgacatcatg cttcgttgtc tcctgttgtt ctgcgtctga aacaaacttt
     1981 atataatcaa accgctgcag cgcagacaat tcggaaagcg tgacggacac accgttatat
     2041 tcaaattgtt ctgttttcag aaacatgtat tacctccgtt taccctgcag cgcccgcttc
     2101 agtaacggtg acttcagcca ctgcggcgaa ctgaccattt ccgctcacca cagggatctg
     2161 caccttacct gtcgccacgc cgtttaccgt aattgtcata tctttcacac taatggtggc
     2221 tttcgacgga tcggcggaaa ccgctctgaa cgtcttgtcg gttgcacttt ccggctcaaa
     2281 agaaaccgtc agggtggttg ttttcccttt tgccaccgta ccggatgtcg gtgtcacctt
     2341 aattgcagtg gccggcgtaa tttcgctgcg ttcttccgcc acggaaggtt tgcccacgtt
     2401 agtgactttc accgtgcggg tgatcacttc tttcgccgtc acggccttac cgatactgct
     2461 gacccagcca cggaacacat ccaccgtgcc atttgggaaa cggattttat aggcccgcac
     2521 atccccgctt tcaaaccagc ctataagccc tttctgacct tcttctcccg gtttccaggc
     2581 cagcgtaaaa ctggtatctc ctgcagactt ctgcccctgc ccggtcgcgg tccagtccgc
     2641 gtcttcatca tccaggtagt tatcatcgta gggttctgcc gtcatctcgc ccggcgtcag
     2701 atccttcacc ttagccagtc gctgccagtc atcgtctgac aacgggtttg cataagcatc
     2761 acccttgccg ttgtaaaccc acagagtggt accggcacct tttaccggct caaggggatt
     2821 tggtgttgcc atatcgtcct cacatctcgt aggtaatttt ccacaggaga tctgccgatc
     2881 cccacatcat aaactcatca tcccggcggt agtcataccc ctgaagattc atcttcagca
     2941 gtaatgcact gagcccggga actgcctcca gcgcaggaag aattttttct tccatccaca
     3001 tatccagtgc cgagtccggt tcttttgccc tgagaaaaac ctcaatatgc agtgtcgcct
     3061 cccaggttcc ctcatcaacg aactcgtcag cagcagacgc atctgtcagg taaacagcaa
     3121 cagcaggcag ttcctgttca tcaataaaaa ccgggcgacc gtcaaaccag ctcaccctct
     3181 cagaaatatt ttctttcagg gcagacagaa ctgccgcccg tatttcacgg tgtttcatac
     3241 accctcccag tcattttctt ttcagcacca ggcgtaactg atgcgtcatg gctttcatca
     3301 tctgcgccgg taatttttcc cggtacatcc ggtcccgttc acgttcaaag gtttctgcca
     3361 gcggtccggc agtcgtaatc ttcaccactt cgatcggcag acggtggcgt ttcggcctcc
     3421 ctttgctgtc cgcgccggtg gatgatgatg cccacggcat acgctgcatc acatgccagc
     3481 gtccgttagc cagccgggtg ataaaggcgt ccgggatccg tcttttcccc acaatcagca
     3541 cactgccgcc ccctttcagg gccgcacgct gtcctttctt tctccgtttt ctgcgggaaa
     3601 gtcgaacgcg ggcctccccc agtttgatgg cgggcaggtt gccggtattg atgtaaacct
     3661 ttgcgtaaac cttatccggt cgtgccggac ttaaccggat gcgggcacgg ataagacgac
     3721 ggggaacggc cagctccctg gcaactgaag aggccgtttt cgcaataatg gcccccgcca
     3781 ctcggttcag tgtcgtggca gaggcccggg gaacggcacg gcgatcaatt gcatccagat
     3841 ttttcatggc ctgcgccaga ccttttattg ccatactcat tcctgttcga caaaaatccg
     3901 gggtttaccg ttgtacgtgt cataacgggt caccgtcagt gtgcgccccg caaacacaac
     3961 aacatcatga cgggccggac ggtaccgggc tgaaaacacc accagtgaca actggctgcc
     4021 cgaaagcgcc cccatctccg cggactcttc ctccggcatc acgtcgtaca cgacgccgtt
     4081 aatctccgcc tgtttgccca tcacccgaac ggtcgccacg tccatccggc aacacattcg
     4141 cgtaaacaga tcagacattg atttttaccg ccacagtggc gctgtttgca ggggcatttt
     4201 cccaggctac ccccgctgcc accgcaccgt ctgcagccag ctgcacaacc ccgtccttca
     4261 gataaaccac cgcgccggac tgaatgtcgt cagcagactg tttgggcagc aggaacacgc
     4321 cttcggcaaa accgtcaccg gcctcaccgg caggaatatc ggtaatggcc acggccacca
     4381 tactgccgac caccaccgca gcaccgctca ggatggtctg atctccggca ttcaccagtt
     4441 caatggtggt accgtcctgt acaaaatttt tcgccataat gctgtttctc cggacagccc
     4501 ctgtggggct gtttttcagg cataaaaaaa gccctttcgg gcagtgattg tgataacgcg
     4561 gttatcaggc caccgacgaa cgcaccagcc cgcgccagtc aagtggtgcc actccggcat
     4621 caatacggat ttttgtggca atgccgtcag tggtgaaacc ttcctgctga tcaatgtatg
     4681 gcgtgtccac accatccagc caggccactt caatggtgtc agtgccctgt gccgccgcca
     4741 gataccaggt tttcgggtct gccgcatcaa gacgcgcttc tgcaatcacc tcagcaaagt
     4801 tctggatagg gttaatgaca ccggcgtttg catccgcccc tttcacactg gccgatttga
     4861 tggtctggtt cgccaccgtc tccagtgcca ccggtaccag cataaaggcc ggacggatat
     4921 tcagggcacg atcgccttct ttctgcaggc gcatcatctg acgggccgca tccagtccgg
     4981 aaacggagat ccccccggtg gcaatatttt tgtgatcggc atggaacagc gccttaccgt
     5041 cggacagttt cgggttatcc gtcagcacct tgtagaccag gtcaccaatc gttgccttcg
     5101 ccgcacgccc catcttcatc ggcacgtcca ccagcatatt cagatcatca ttgataatgg
     5161 cctggcgggt gatggagaaa atctccccgt aagtggccag tgcaatggtc tccttgcgat
     5221 ctgaggtggt gatgtattta tactccgccc cctcacgaac ctggcgcaga gaaccaaaac
     5281 cgcccatccc cacgcgatac gctgttttga agtctgacag gcgtccctta cgggtccact
     5341 tctggaaggt ttcttctgat tcctcccagc cctggatcag ccccttgttc gacacatcca
     5401 gcagaatatt gccaaaatca gaggtgctgt gcgtcagcgc cagcccgacc atctgcatgg
     5461 ggttataact ggccacccca ataccgcgct ccgtcagtga catgcgagcc cattcacgca
     5521 gggtcatccc gttataggcg ttatccttct cgacattttc aaatccggca cgggccagca
     5581 tcgcctggcg gatcccgtcc cccacaaaat tgccgtttcc ggcataaata tgggccggtg
     5641 tatttttgtt ggtcggcgag gactccttgc ccatttcatt cagcagacgt tcacgggcca
     5701 tttccagcga acagtcagga tcagccacgc actgtgcctg aagcgtctga tagcgaccgc
     5761 cgaacatggc aaacagatcg ttaatgcctg acatgcgggc tttctgctca gccataacgc
     5821 gggcgcgaat ggtcgcctca tcagacactg ccggtaccgg tgatggttct gttaccgccg
     5881 gtgcagggat tgtcactgtg gtatcacgcg gggcactgtt gcgtggcgga gtaatcatgt
     5941 ttcggatgga ttccggcatc tttttaaatt cctctgtacg ttttgactga atacatgcca
     6001 ttgcctcaac agcgggtgtc acctggtcag caaatccgtg tgccagacat tcggcaccgg
     6061 acatccaggt ttcatccgcc agcatggcgg caatttcatc ggtggttttt ccggttttct
     6121 gcgcataggc tggcaacagt accgattcga ctttatccag caaatcggca taactgcgca
     6181 tatcctcagc atccccgcca ctgaatcccc atggcttatg gatcatcatg aaggcatttt
     6241 ccggcataat gaccgtatca ccggccatcg caatcacaga tgccatcgag gcggcaacgc
     6301 catccacata cacggtaatg gtcgccccct gatttttcag ggcattaaaa atggcgatgc
     6361 cttcaaagac atcgccaccc ggtgaattga tatggagatt aatgtgggtg atatcaccca
     6421 gtgcattcag ttcgctgaca aactgcttcg cggtaactcc ccagaaacca atctcgtcat
     6481 aaatataaat atccgcgtca cccggccccc cagcttgcat cctgaaccag gatttattct
     6541 tcatgctggc tttcggtgtc gcgctgatac tgtcgttcag ttccggcact gttgcctcct
     6601 ttgtcgttga cggggtcagt atcaaagacc agccccagtc tgctgttttc atcaatttca
     6661 gccttgcggc gacgtttgac ctcatccgga ttgcgcccac cggcacgcac ccagtcagat
     6721 tctgtcgctg caccaccccg gatctgaatt ctccaggctt cagcttcctt aaccgggtcg
     6781 atccacggca tcaccggacc ggaatacgtc gcgttatata gcgttttcat ctccacatcc
     6841 gccggaattt tcagcagacc tgccgcaacc accatattca gccatgtccg gtacaccggg
     6901 cgggttaccg cgccaataaa acagtcctgc aggatcaggt aaccatccgt ggactcgacc
     6961 agctcctgcc gctgggcgct gtaggtgccg ttatagttac gcgccgcact ggaaaaactc
     7021 agacgactgc ctgctgccac tgcacgcaac tggccgttgc ggaaagtttc aaggttggga
     7081 ttgggacggt cagatttgac catgccgata tcctcgccct tgcgcaaatc gtcataaata
     7141 atacccgggg tgatatggac ttcccgctcg gtctctttga tccccggatc ttcatagtcc
     7201 tgtccgtcac ctttacggat atacagtccc agcgccgcag caatacgcgc cgctgtcagt
     7261 tccgcatcct catactcctt aagggcactg atccgcatca gcacccccga taacatggat
     7321 gagcctcgcg tctgatgcag acgacgagtg aacttcaggt ggatcatttt tccggcagcg
     7381 atttctttcg tatcactctg ccggccgctg accggataat ttttataaac cagatatttt
     7441 ttcggtcttc cccactcatc aagaaaaacc ccctgattca gtccggcgga ttcatcagtg
     7501 cgcatgggaa caaaatccgg ctccatcgcc tcaagccaga atggcactcc cgccgtccgt
     7561 tccagaccgt ttcccgcacc actgaccatc tgcgcaaaca cttcaccatc ccgcagccag
     7621 gtccgcagca gtaaacgttc aagcacagga cgggtatact gccctgtcac atccggactc
     7681 acggaccatt cagcccacaa acggcggata tccgcagcca gctcagccgc catttccccg
     7741 ttttttcgta atggctgagg ctccacaata attcccctgg caccaatcac ccgctcttcc
     7801 agcttgtcaa acacaccaat caccaggtca tgattgatat ccagaaaacg ggcctgctcc
     7861 cgcagggaaa ccgcaccgta tttactgagc tgatcagcag agcgattttc ccgccgggct
     7921 ttatgtgtcc gggtcggttt caccgcctca taggccatga ttaacgccct tgaacgcagt
     7981 ctggctgctt tccacccggg ggaaaacacg ccgatcacat catcaataat tgccattaaa
     8041 acctcgccag tttaaatccc ggttttcccc gcctgcggct caccatcgcg gcaagcctgc
     8101 gttcccactc ctgacgtccg gcgcggatct gagaaaggct ttccagcgtc agttgctgcc
     8161 cgttgaagat gacagacttt ccctccagta cggccatttc cgcttcacgg taccgctgta
     8221 tcatttctct ggcttcttct gtgctcacaa ccagcctcct gatgttatcc atggattatc
     8281 ttccgcacgc tccgtccgca gttttttctt ccggcgacgg cgtttttctg ccccggccgt
     8341 cagttccggg gataccgttt caccagaacg ctcctgcggg aagacgagcc acgtttcccg
     8401 ctgtgcccag tccggtgcgg agggccagcg gatcttttcg taaccatgca gaacggcaag
     8461 cgcatccgca taaaccagca ggtcaaacgc ttcgttagcg cccctgcccg gttttcgcca
     8521 ttttccgtca ctgccgcgct cttcataggt cagctcatcg taaaaccacc gccccagcca
     8581 gtcgggaaag tggatatagt tcggccctgg tgtgtcacgc cacagggcat tatttacacg
     8641 atccttaaac gcatccgtct gaaccagcca cagcgcgaca tcgccactgg ctctggcacg
     8701 gcgggcactt ctgccggtat tatccgggaa ggttcggtta atcagcctgt cacggcgaag
     8761 tccatccccc ttgaacagaa acaccctgtt gcccagtccg tcactccggc aacgacgcca
     8821 gaaacgatag gcgttatctg tcaccccggc ttcccctccc gtatccaccg ccatggccat
     8881 cagacgcatg cgcacatccg gatcagaagc cagcggccat gttttatgga acacatccgt
     8941 cagcaacaaa tcccagtcct ccggatatgc cgccggatca accggcagac tttcaccgtt
     9001 gggactgcag cgcagtgaat gccggatgtt gtagcgatca acaatccagc gttccccctg
     9061 ctctccgtat ccggtgatct gcacaacaaa acggcgattt ttaccgccct gtacgtcaac
     9121 cgttgcctca ataaaacgca caccatccgg cacagatcgc cggggaaacg gctcggcacg
     9181 ctgttcaagc agttcacttt tacgctgttc cgtggctgaa cggggcagat agggtcgtcc
     9241 gatatcggtg ttcaccaccg ctttcagggt ctcttcactg ccggttcgct catactcttc
     9301 ttctgccgcc agcagtttaa aaatcagttg ttcccaggtc tgaaacgccg cagctggccc
     9361 ctccatccaa gaagaagtaa ataaaaggta actaaatgat tataaggtac tattgagtta
     9421 tgccccctac aacatagtcc cagatttagt acctgcttaa gcgataaaaa agcaaaaaag
     9481 gaagcaatat taattattgc cgctatgcat gatcacaatg acaaatcaga ggcggaattc
     9541 cgctctcgga actcacatat gagaaacgat atttcttcca ccgataccct ttatttgggt
     9601 atcgcaagta agccccaaaa tctgggtgtt gctcttacta cccaaactac gggtagtttc
     9661 cgtaatttca tgatcgagtt gcagatctgc aactccccca ccagccaacg caattttgcg
     9721 ttatcgtgaa tatcaacaag ttacctccgc agccgttccg gcgtcttcca ctggtaagta
     9781 tttttcgcgc tctccctccg ttgttgagaa cggcgacggt atgccagcaa ctcaaggact
     9841 ctggttcgta tattgcgcat atccacatca ttaagctgga taccatcacg gcgcatcacc
     9901 tccaccacta cacgcacata attatctgcg gtcatgctgt ccggctgcgt ggcctgttcg
     9961 tcaacctgct ggctgattcc ggcgacgcgg cggattaatc ccagtatttc ggcttctgtc
    10021 attgtgcccc catcgcgctg gtgaagaaaa caagctcaga ttttttgtaa agaatctgtc
    10081 acgctaaaag atgtcgaaca aaaaacaacc ttcttcatc
//
LOCUS       DABSYT020000137         9849 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.137, whole genome shotgun sequence.
ACCESSION   DABSYT020000137 DABSYT020000000
VERSION     DABSYT020000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.137"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            162..1505
                     /locus_tag="ID725_RS21705"
                     /old_locus_tag="pgaptmp_004341"
     CDS             162..1505
                     /locus_tag="ID725_RS21705"
                     /old_locus_tag="pgaptmp_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGV7852807.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            1527..1847
                     /locus_tag="ID725_RS21710"
                     /old_locus_tag="pgaptmp_004342"
     CDS             1527..1847
                     /locus_tag="ID725_RS21710"
                     /old_locus_tag="pgaptmp_004342"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGV7852808.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            1859..3340
                     /locus_tag="ID725_RS21715"
                     /old_locus_tag="pgaptmp_004343"
     CDS             1859..3340
                     /locus_tag="ID725_RS21715"
                     /old_locus_tag="pgaptmp_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGV7852809.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(3391..4122)
                     /gene="artJ"
                     /locus_tag="ID725_RS21720"
                     /old_locus_tag="pgaptmp_004344"
     CDS             complement(3391..4122)
                     /gene="artJ"
                     /locus_tag="ID725_RS21720"
                     /old_locus_tag="pgaptmp_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGV7852810.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
     gene            complement(4413..5081)
                     /gene="artM"
                     /locus_tag="ID725_RS21725"
                     /old_locus_tag="pgaptmp_004345"
     CDS             complement(4413..5081)
                     /gene="artM"
                     /locus_tag="ID725_RS21725"
                     /old_locus_tag="pgaptmp_004345"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGV7852811.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(5081..5797)
                     /gene="artQ"
                     /locus_tag="ID725_RS21730"
                     /old_locus_tag="pgaptmp_004346"
     CDS             complement(5081..5797)
                     /gene="artQ"
                     /locus_tag="ID725_RS21730"
                     /old_locus_tag="pgaptmp_004346"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGV7852812.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(5804..6535)
                     /gene="artI"
                     /locus_tag="ID725_RS21735"
                     /old_locus_tag="pgaptmp_004347"
     CDS             complement(5804..6535)
                     /gene="artI"
                     /locus_tag="ID725_RS21735"
                     /old_locus_tag="pgaptmp_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGV7852813.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(6553..7281)
                     /gene="artP"
                     /locus_tag="ID725_RS21740"
                     /old_locus_tag="pgaptmp_004348"
     CDS             complement(6553..7281)
                     /gene="artP"
                     /locus_tag="ID725_RS21740"
                     /old_locus_tag="pgaptmp_004348"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGV7852814.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(7499..8014)
                     /locus_tag="ID725_RS21745"
                     /old_locus_tag="pgaptmp_004349"
     CDS             complement(7499..8014)
                     /locus_tag="ID725_RS21745"
                     /old_locus_tag="pgaptmp_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGV7852815.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            8638..>9849
                     /locus_tag="ID725_RS21750"
                     /old_locus_tag="pgaptmp_004350"
     CDS             8638..>9849
                     /locus_tag="ID725_RS21750"
                     /old_locus_tag="pgaptmp_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852816.1"
                     /translation="MEYRFSWVIFNMINNISDQASSFPCTQLNQSDNFLDSLREFFAI
                     LNSSRKGELSTWDTIYLHLILVINADSDLIKNDVLLAENIPTADYQFNTFFSNAFETD
                     VKKYLGKSEDNEVEIKTGNERISIGIRNRSNGKLERQKFLFPLDYENKLQHQLDKHFT
                     IESHPLLYRHTMGSKIANVIFEKLYSRIDFNKEQYISFIKDAFLHFYDYSRRYAINEN
                     IAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEESFETALDVEHTIVLGFADDNFETK
                     PVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVFM
                     SLPVPVATMVREDASNLINLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNES
                     ILKKCCFENGSL"
BASE COUNT         2603 a         2161 c         2388 g         2697 t
ORIGIN      
        1 aatcacaaat aatcaaataa gatctttaat tgaactgttt tgattactac agtgcactta
       61 ccgaatacat acagaggcat tgcttagcga aacacaaaat ttcctgagac attattaacg
      121 tcagcagaag gttcatgcat tgacaacgga aatgtacgcg tatggagggc gttccgacga
      181 tgtttgccaa attcatagat gttattcaaa cgtttttaac tgaaccggca attttaattg
      241 gtttgctggt gggtattggt tatgcccttg ataagaaatc gccaataaaa attattaccg
      301 gcatggtcag cgccatggtg ggattaatga tggtgttatt tggtggtttc cagttttccg
      361 caacatttaa accggtagca gaagccgtaa gtaaagccta tggtgttcac ggatatttaa
      421 tggattccta cgcaatgaaa gcggcaacgc aaattgcatt gggggataat tttggttatg
      481 tcgggtatgt atttgtactg gcgtttttta ccaacctgct gctggtatta tttggtcggt
      541 atactggtgc caaagggata tttttgacag ggaataccgg tgtttcacat tctcaggcgg
      601 tattatggtt gattgtcttt tggctgggtt ttagctggac tacatccatt attattgccg
      661 gaatattaac gggtgtattc tgggcgttct ccaccacgct cattgttaag cccattgcca
      721 aagttaccaa agatgccggt tttaccattg ctcataacca gatgttggga ttgtggtttt
      781 tctccaaatt cgcccataaa tttggtgacc ctgaaaagca cgatgctgaa aacctgaaac
      841 ttcccgggtg gctggcgatt tttaaccata acgtaacggc tatcgccatt gtgatgacgc
      901 tgtttgtcgg tggtttcttg ctatcaacgg gtatcgataa tgtccagtta atggcgaaag
      961 gcaagccctg gtacatctat atcatcaacc ttggcttaca gttctccatg tatatggtca
     1021 ttctgctgca gggtgtgcgc atgatggtgg gcgaaattaa cggttcgttt aaaggctggc
     1081 aagatcgctt tattcctaac gctattcctg ctgtcgatgt tgcagcgctg ttaccttttt
     1141 cacccaatgc cgcaacgtta ggcttcgtct tctgtacttt tggcaccatt ttttcgatgg
     1201 ggatcttatt actggttcac agcccaatta tggtattgcc tggctttgta cctctgtttt
     1261 tctctggcgg tccaattggc gtattagcaa accgcatggg cggataccgt tccgtaatta
     1321 tctgtacgtt cttgctgggt attattcaga ccttcggtac ggtgtgggct attccgttaa
     1381 ccgggcttgc tgaaaatggc gtcggctgga caggaatatt tgactgggca accgtatggc
     1441 ctgctatttg tgaagttctg aaatttatcg ctgcaacatt ccatcttggt ccttacgcgg
     1501 gttaattaaa tattaagggt ttaaaaatga aagatacaat taatattctg tttgtctgtg
     1561 gttacggtgt aggcagtagc gttatgttac aaacggtcgt taaaaaagcg ctgaccaaat
     1621 atgatttcaa agttgaaatg gaacataccg ccgcaggtga agtcggcgga tttaccgact
     1681 gggcggatat ttttgctatt tccaaaaaat tactcgatgt ggttagtctt gacccgaaac
     1741 aagggcaata tttgattccg atagagaata ttatggacgg tgaaactatt ggtcagcaaa
     1801 tttatgaagt ggttcagcaa cattttccgc atttaattaa tcaataaggt agggcgtaat
     1861 gagagcgatt attctgctgt tcgatagtct gaataaacgt tatctgccac cttatggcga
     1921 tgcgttaacc aaagcgccta atttccaacg tctggcggct catgccgcca cctttgaaaa
     1981 cagttacgtc ggcagtatgc cgtgtatgcc tgcccggcgg gagttgcata ccgggcgatg
     2041 taacttcctg catcgggagt gggggccgtt agaaccgttt gacgattcca tgccggagct
     2101 gcttaaaaag gcggggatct atacccacct gataagcgat catttgcatt actgggaaga
     2161 tggcggcggg aattatcata atcgctacag ttcgtgggag attgtgcgcg gacaggaagg
     2221 cgatcactgg catgcaagcg ttgcgcaacc gcctattccc gaggtgctac gggtgccgca
     2281 aaagcagacc ggtggcggtg tttctggtct gtggcgccat gactgggcaa accgggaata
     2341 tattcagcag gaagcggatt ttccacaaac taaagtgttt gatgcaggct gtgcctttat
     2401 ccacaaaaat cacgcggaag ataactggtt attgcagatc gaaacgtttg atccgcacga
     2461 gccgtttcac acaacggaag agtatctttc cttgtatgaa gataactggg atggaccgca
     2521 ttatgactgg ccgcgtggcc gggtgcagga aagcgacgag gccgtggagc acatccgttg
     2581 ccggtatcgt tcgctggtgt cgatgtgcga tcgcaacctg gggcgcattc tcgacctgat
     2641 ggatgagcac gatctgtggc gagataccat gctgattgtc ggcactgacc acggcttttt
     2701 actcggtgag catggctggt gggcgaaaaa ccagatgcct tattacaacg aagttgccaa
     2761 taatccgttg tttatctgga acccgcgcag tggtgtaaaa ggagagcggc gacaggcact
     2821 ggtacaaatg atcgactggg cgccaacgtt gtatgacttt tttcaacagc cagtgccgcc
     2881 cgatgtgcag gggcaaccgc tggcgaaaac ggtcagtcac gatgaaccag tacgcagctc
     2941 ggcgatgttt ggtgttttca gtggtcatgc taacgtaact gatgggcgtt atgtgtatat
     3001 gcgtgcagcg ctgccggggc gtgaggatga tattgccaac tacacgttga tgtcctgcaa
     3061 aatgaacagc cgctatccgg tggatgagat gcgggcttta tcgctggccc caccgtttcg
     3121 ttttaccaaa gggttacagg tattacgcat cccggcacag gaaaaatata aggggttgaa
     3181 tcagtttggt catttgctgt ttgatctgca aaacgatccg cagcagctac atccgattca
     3241 tgatgatgtg atcgagtccc ggatgatcgc gttgctgatt cagttgatga aagataatga
     3301 tgcgccgcca gagcagtttc agcgcctggg attagcgtag agaagtgatt ttgccggagt
     3361 gggtgttcgc ccactccggc aaatgaataa ttattgcggg aaccactgat cgctgatttg
     3421 ttgatatgta ccgtcagctt taattgctgc cagcgcgtta ttcagttttt ccagcagagc
     3481 tttgttatcc ggacgtacag cgatacccag accggtgcca aagtattgcg gatcggtcac
     3541 tttttcagta gcaacgccca gttgcggatt ggttttcagc cattcattaa ccaccgcagt
     3601 gtcaccaaat acgccatcaa tacggctatt tttcagatcg ataaaggcat tttgataact
     3661 gtcataggag accgttttca cttccgggtg ctgatcctga atatatttct ggtgcgtagt
     3721 accgttttcc atcccaatgc gtttgccttt caggtcggca aacgttttgt aggtatcttt
     3781 tttggcaatc acgacggctg agttttcgta atagggcgtg gtgaacgata cctgcttgct
     3841 acgatctggt gtgatatcca taccggagat tacggcgtca tatttcctga atttcagtga
     3901 cgggatcagg ctgtcgaacg cgtgattggt aaaagtacat tctgcctgca tctgtttgca
     3961 taacgctttt gctaggtcga tatcaaagcc gacgatctgg ttattggcat ccatcgactc
     4021 gaacggcgga taggtcgctg atacgccaaa attgattttc tctgcggcag aagcgccgaa
     4081 cgcacaggat gccagtaatg cggcaagaac taactttttc atgatggaac tcccgtctgt
     4141 caatcttatg attattggcc gtgtctgcgg catgggataa caatgccatc aagtgaattt
     4201 atatgcaata aatatgatta aataatttgc atgaaataaa aaaagacgga caacttagtg
     4261 agttgtccgt ctttattata agaatttatg cactatgtag gccggataag gcgtccacgc
     4321 cgcatccggc acaggcaccg tgctgatgtc tgatgcgacg ctggcgcgtc ttatcagacc
     4381 tacaaaaccc ccggcgaatg tacgcagcca cattaattcc ttcgctcaaa cgccagcgct
     4441 ttgcgctcga tcagacgcat catcagcgtc agcaggccgt taacgaccag ataaataatc
     4501 cctgccgcgc cgaacaccat gacatcgtag gtgcgtccgt acaacaactg gctgtatccc
     4561 atcacttcca tcagcgtaat ggtgtaagcc agagaggtac ttttgaacac cagcaccact
     4621 tcgttggaat aagaagagag cgagcgttta aaggcatacg gcagcaggat cgccagcgta
     4681 tcttttttgc tcattcccag ggcgctacag gactgccact gaccttccgg gatcgcacga
     4741 attgcaccgt aaaacagctg cgtggtatac gccgcactgt tcagcgacaa cgcaatcagc
     4801 gcacataacc acggttctga caacaaatgc cacagtgccg gatactcctg caacgtcggg
     4861 aactgacccg gcccgtaata aatcaggaag atctgcacca gcagcggtgt accggtaaac
     4921 agcgtgatat aaccccgcac cagccacacc agcaccggcg ttttcagcgt caggatgatg
     4981 gtaaaaatca acgccagaat cagtgccaca atcagcgagg caacggttag cgtcaggctg
     5041 gtgtgcagcc ctttcatcag ttcgggtaaa tactcaaaca ttagctgggc ctccgctcaa
     5101 aacgtgtcgc gcgcaggtca atgcgtttga gaatgtactg actgagcagg gtgatcacca
     5161 ggtaaatcgc cgccgccaca atgtaccagg taaatggttc ctgggtacga gtagcgatgc
     5221 ttttggtttg cagcattaaa tcattcacac taatcaaact gaccagcgcg gtatctttca
     5281 gcagcaccag ccactggtta ccgaggccag gcagcgcatg acgccacatc tgcggcatca
     5341 ccagacggaa aaagatagcc gatttcgaca gccccagcgc ctggccggat tcccactgac
     5401 ccatcggcac cgctttcaac gcgccgcgca gcgtttgcga ggcataggcg gcatacagca
     5461 gtgacagagc gatgacacca caaaggaacg ggctaacgtc gaagttttca atctgcatct
     5521 gcaccgggat ctctacaatc ccaagattga tagtgaagcc atccgaaagt gtcagcagca
     5581 gctgcgagga gccaaaatag ataaacagca ccaccagaat ttctggcagg ccacgcagaa
     5641 tggttaccag cgctgaacct gcccacgcga caggacgcca tttcgcagac tcccataccg
     5701 caaagaacat cgccagcgcc agcccgacaa tcaatgcaca aacggcaagg ccgacggtca
     5761 tcccggcggc gcttgctaaa ggaaaaaatt cattcatcag gaattacttc tggaaccatt
     5821 tgttgtagat ggtttcgtaa gtgccatctt tcttcacttt ttccagcgca gtgttgagtt
     5881 tctgctgcag ctcagtgttg ccctgacgta ccgcgatgcc gaggccagta ccgaagtaat
     5941 ctttatcggt cactttatcg cccactgccg ccagtttcgg gttatctttc agccattcag
     6001 ttaccactgc ggtgtcacca aatacgctgt cgatacgacc gttttgcaga tccagttttg
     6061 cgttctggta gctgtcatac ggcacggtgg tgatttccgg gtgcttatcc ataatgaatt
     6121 tctggtgagt cgtaccattc tgtacgccga cttttttgcc tttcagctga tcaacgctgg
     6181 tgtatttacc ctgctgaccc acaaacaggg cagagttgtc atagtacggc gtggtaaaca
     6241 gtacctgctt ttcacgttcc gcggtgatat ccatcccggc catcaccgct tccacgcgac
     6301 ggaatttcag gcttgggatc aggctgtcaa atgcctggtt agagaaagta caggtcgcat
     6361 caatctcttt acacagcgcc tgtgccaggt cgacgtcaaa accaacgatc tggttgtttg
     6421 catcaatcga ttcaaacgga ggataggagg cttcggtagc aaaacgaatg gtttcggcag
     6481 ctgtggcgga aagactaaaa cctgcaatta acgcggcaat cagaactttt ttcattgttg
     6541 ttatcccgaa tcttagtgag agagatagtt tttaaatgct tcggtttgcg gctcggtaaa
     6601 gcagctcgcg tcgccttgtt ctacgatatg accgttttcc atatacacca ctcggctggc
     6661 ggttttacgc gccacttcaa cttcgtgggt gacgatcacc tgggtaatat tcgtttctgc
     6721 cagctcacga atgatgctga cgatttgtgc cgtaatttcc gggtccagtg cggcggtcgg
     6781 ttcatcaaac agcagtacct gcggttccat cattaacgca cgggcaatag caacacgctg
     6841 ctgctgacca ccagaaagat gcagcgggta acgatcgcta taaggtttga gacgcagacg
     6901 ttccagcagt ttttctgcac gggccagcgc ctgatcttta ctcaacccca gtacacggca
     6961 gggcgcttca atcaggtttt gctgcacggt cagatgcggc cacaggttgt attgctgaaa
     7021 caccatgcca acgttacgac gcaaatcgcg aatcgctttg tctgagggtg ttttggtgaa
     7081 atcgaaatgg ttgcctgcaa tgttgagcgt accggagcgc ggcatctcaa gcagattgag
     7141 tacacgcagc agcgagcttt tacccgcgcc gctggggcca agtaacacca gcgtttcgcc
     7201 ctgtgggcaa tccagcgtga tatcgaacag cgcctgatgc gcgccgtaga agcaattaat
     7261 gccgtttaat tgaatactca ttgacactcg tatactggca gtctgatagc tattgaggtc
     7321 gaagatagta cctttgacag aataattatg caatatttct gctttaaaag ttaaaagcaa
     7381 agcgcattat tcaataaaca tagcacaaaa taacgggggc ggtggtcggc gagcataaat
     7441 gtcggcattc ctcacgaaat gccggacaat ttacggggtt tattggttga tcaaggcgtt
     7501 agcgattctc gatggactga cggagcgtac ccgccgtggc atgaacgcta ccgcctaagt
     7561 agcgcacatc gtcgatcacc caacactggc cttcctgaat cattaacact tcatcctgcc
     7621 agccctggtc accctgcttg agatccacgc gcaatggaat gttacgggca tcacgattag
     7681 ggatagtcga tgcactggca acgtgggcgc tgtctggcaa ggtggttcga ctggagaacg
     7741 gatcactgct taacaattca cgatgactat tatcgcggct ggcatcgcta agtagggttg
     7801 ccagtttgtc actcaaatag gggcgcaggg cggtaatgtc attgctgcga tgttgaatgt
     7861 ggtagtcata gaattgttgg gccacgctgt caggcccccc ctggacacag gaaccggtgc
     7921 ggggaccatt atctttataa gctggcgtga cggtggtaca ggcactcagc agcagtgcac
     7981 atggaataag tagagatagt gaacggtagc gcataatcat ttccttatta gcttgctgtc
     8041 tgcgtcacac tacgaccaaa cgctatcaga ttatttaata cattgttttt aatacaattc
     8101 tgaaacaatc tgaacacctg attgggtttg agtccattgc tggcgaccca ctcaaggttg
     8161 ctcgtagctg acttacttaa attacccaaa caatagacag tgtaaaccat ttgtgaggtt
     8221 gagcgtgtta tatggctgaa atgatcattt aaggtaaata tgatagttct gaaatggtgg
     8281 tataaatcct cctgtagagg ttaagagcat ctgcaagaag ccagtttcac atttataacg
     8341 cgtgataaat gaacgcggtt attgtctaat cattacaaat ttttcacatg cgataagaga
     8401 tcctgctatt aagatgattg ccaaaattac gttgtatttt tgaaaatgag cgaaatttga
     8461 tagtcccagc tttgactttt gataatatta tttatgctac agttgtgggt ataaaatata
     8521 atctttttga acaagtgaat ttgagctaag aataaatcaa gttgtttcag tgtttcgtta
     8581 attaatatga gtataaaagg tgttgtgctt ttataataat aaagcgcgag gagttcggtg
     8641 gagtatcgtt tttcttgggt gatttttaat atgataaaca atatatctga tcaagcgtca
     8701 tcatttccat gcacacaatt gaatcaaagt gataatttcc tcgattcact gagggaattt
     8761 ttcgcgatat taaattccag tcgtaagggt gagttgtcta catgggatac catctacttg
     8821 catctgatat tagtaataaa tgctgacagt gatctcataa aaaatgatgt gttgctcgcg
     8881 gaaaatattc caacagcgga ttatcaattt aatacatttt ttagtaatgc tttcgagact
     8941 gatgttaaaa aatatctggg taaatcagaa gataacgaag ttgaaataaa aacgggtaat
     9001 gaaagaatct ctattggaat acgtaacaga tcgaatggaa aacttgaaag acaaaagttt
     9061 cttttcccac tggactatga gaataaatta caacatcaat tagacaagca ttttacgatt
     9121 gaatcgcacc cattattata caggcatact atgggtagta agattgcgaa tgtgattttt
     9181 gaaaaattat acagtcgtat tgactttaac aaagaacaat atatatcctt tataaaggac
     9241 gcgtttcttc atttttatga ttatagcagg cgatatgcaa ttaatgaaaa tattgcaaaa
     9301 gacgctgtaa caaacaatat cgcattgatg agcacttttt atgacagtga taatacgtca
     9361 ggtgaggttt taaataatga ttttacagaa gagtcatttg aaactgcgtt agatgtagag
     9421 catacaatag tgcttggatt tgctgatgat aattttgaaa caaaacctgt tcattatcag
     9481 gatttattaa cacgatttag tgcctttcaa gataccgtgt ttaatctgtt tcctgaaatg
     9541 cactcttcgc attacaacga tatttgtagc gtaagtgtgg atatgacaaa gggaacgcaa
     9601 tgcatgattc acttgatggt gaatgaagaa gttttcatgt ctctgcctgt tccagttgcc
     9661 acgatggtac gtgaagatgc aagtaattta atcaatctta aaacactact caatgatggt
     9721 tgctttatta aatatagtca ttttaatgac gtggcattaa taaagcaaaa cataagtaat
     9781 ctctatttat cacacactgt tgtgaatgaa agcatattga aaaaatgttg ttttgaaaac
     9841 ggcagttta
//
LOCUS       DABSYT020000138         9601 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.138, whole genome shotgun sequence.
ACCESSION   DABSYT020000138 DABSYT020000000
VERSION     DABSYT020000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.138"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            236..871
                     /locus_tag="ID725_RS21755"
                     /old_locus_tag="pgaptmp_004351"
     CDS             236..871
                     /locus_tag="ID725_RS21755"
                     /old_locus_tag="pgaptmp_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGV7852817.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKKLTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1384..2634
                     /gene="frc"
                     /locus_tag="ID725_RS21760"
                     /old_locus_tag="pgaptmp_004352"
     CDS             1384..2634
                     /gene="frc"
                     /locus_tag="ID725_RS21760"
                     /old_locus_tag="pgaptmp_004352"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGV7852818.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            2688..4382
                     /gene="oxc"
                     /locus_tag="ID725_RS21765"
                     /old_locus_tag="pgaptmp_004353"
     CDS             2688..4382
                     /gene="oxc"
                     /locus_tag="ID725_RS21765"
                     /old_locus_tag="pgaptmp_004353"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGV7852819.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            4452..5396
                     /gene="yfdV"
                     /locus_tag="ID725_RS21770"
                     /old_locus_tag="pgaptmp_004354"
     CDS             4452..5396
                     /gene="yfdV"
                     /locus_tag="ID725_RS21770"
                     /old_locus_tag="pgaptmp_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGV7852820.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            5470..6611
                     /gene="yfdE"
                     /locus_tag="ID725_RS21775"
                     /old_locus_tag="pgaptmp_004355"
                     /pseudo
     CDS             5470..6611
                     /gene="yfdE"
                     /locus_tag="ID725_RS21775"
                     /old_locus_tag="pgaptmp_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(6667..>9601)
                     /gene="evgS"
                     /locus_tag="ID725_RS21780"
                     /old_locus_tag="pgaptmp_004356"
     CDS             complement(6667..>9601)
                     /gene="evgS"
                     /locus_tag="ID725_RS21780"
                     /old_locus_tag="pgaptmp_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGV7852821.1"
                     /translation="SRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALT
                     NEVRYEVSQNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENG
                     SVRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVL
                     FAEAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNAS
                     AAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELK
                     DIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWG
                     FYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTAD
                     YYKNAMLPLENSESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTL
                     CNLPASEHAVYICGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPIS
                     SIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWV
                     DIPTLVQNTCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFT
                     TEGAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGL
                     GLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLS
                     ILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGF
                     ELTRKLREQNSSLPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQV
                     AHIVPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQ
                     CIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVF
                     CQQNN"
BASE COUNT         2641 a         1991 c         2193 g         2776 t
ORIGIN      
        1 taattaatca atatttacat cgatataata aatgacatct ctttgtggta cataagaata
       61 gttctctgtg acaggaagca tattcctaca attgtaagac taaaatactt cttgcgataa
      121 taactacaac tgtaagataa ccctttcaaa atgaccgttg ctctctgatt tctcatttca
      181 tgctcaccca atatgatggc ggcgttttct aaaactgtta aagaatgagg taagtatgaa
      241 acgtttaatt atggctacga tggtcacagc tattctggca tcttcaactg tatgggctgc
      301 tgataatgct ccggtagcgg cacaacagca aactcagcaa gttcagcaaa cccagaaaac
      361 ggcagctgca gcagagcgta tctctgaaca aggtttatat gcgatgcgtg acgtgcaggt
      421 agcgcgcctg gctctgtttc atggcgatcc ggaaaaggca aaaaaactga ccaatgaagc
      481 ttcagctttg ttgtcagatg acagtaccga atgggcaaag ttcgctaagc caggtaaaaa
      541 gaccaatgtc aatgatgacc aatatattgt cattaatgca tctgtcggaa tttctgagag
      601 ttatgtagcg acacccgaaa aagaagccgc gataaaaatt gccaatgaaa aaatggcgaa
      661 aggggataaa aagggcgcaa tggaagagct tcgcctggcc ggtgttggtg taatggaaaa
      721 ccaatatctg atgccgctta aacagactcg taatgcactt gctgatgcgc agaaactgct
      781 tgataaaaag caatactacg aggcaaacct ggcgcttaaa ggtgcggaag atggaatcat
      841 cgtcgatagc gaagcactgt ttgtgaacta atgctgacag gcagtatatt gaggtaaagg
      901 atttaactcc ctccagccga caatttttta tgaccacaat acgacaggtt gtggtcataa
      961 aattatttgg cgctcgctgt tctataaact cgcattttat attcctcaaa gtaaaccgca
     1021 ataatcccgt gtcatcacaa atcatttaat atgttatatt ttatggtacg ctctaaaaag
     1081 cgtaatcata cccttacttg taaatcaccg gcgcttctta cagttgtaag aataacatca
     1141 caaatgtcgc gactctctct gattaccacg aaccaatatt tatcatattc ttcttttcgt
     1201 cctcactact taccatgcag aaaggaattg gaagtagcga tgaaatgatt ttcagcactt
     1261 gatacaggaa tttaaaaata tcgagtgccg ctatgacgat attttgcgga ttatttatag
     1321 agggcgttat gccttaaata aacgctaaat taacacatat ttaattaact aataggtatt
     1381 gctatgtcaa ctccacttca aggaattaaa gttctcgatt tcaccggtgt gcaatctggc
     1441 ccatcttgta ctcaaatgct ggcctggttt ggcgctgacg tcattaaaat tgaacgcccc
     1501 ggcgttggtg acgtaacgcg tcaccagctg cgagatattc ctgatatcga tgcgctttac
     1561 ttcaccatgc ttaacagtaa caaacgttct attgagttaa ataccaaaac agcggaaggc
     1621 aaagaggtaa tggaaaagct gatccgcgaa gctgatatct tagtcgagaa ctttcatcca
     1681 ggggccattg atcacatggg cttcacctgg gagcatattc aagaaatcaa tccacgtctg
     1741 atttttggtt cgatcaaagg gtttgatgag tgttcgcctt atgtgaatgt aaaagcctat
     1801 gaaaacgttg ctcaggcagc gggtggcgcg gcatccacta cgggtttttg ggacggtccg
     1861 ccgctggtaa gcgctgcagc gttgggtgac agcaacaccg gaatgcattt actgatcggt
     1921 ttacttgctg ctttgctgca tcgcgaaaaa acggggcgtg ggcaacgagt caccatgtca
     1981 atgcaggatg ccgtattgaa cctttgccgc gtgaaattac gcgaccagca gcgtctcgat
     2041 aaattgggtt atctggaaga atacccgcag tatccgaatg gcacatttgg tgatgcagtt
     2101 ccccgcggag gtaatgcggg tggtggcggt cagcctggct ggatcctgaa atgtaaaggc
     2161 tgggaaactg atcctaacgc ctatatttat ttcactattc aggagcaaaa ctgggaaaac
     2221 acctgtaaag ccatcggcaa accagattgg attaccgatc cggcatacag tacagcccat
     2281 gcccgacagc cacatatttt cgatattttt gctgaaatcg aaaaatacac tgtcactatt
     2341 gataaacatg aagcagtggc ttatttgact cagtttgata ttccttgtgc accggtttta
     2401 agtatgaaag aaatttcact tgatccctct ttacgccaaa gcggcagtgt tgtcgaagtg
     2461 gaacaaccat tgcgtggaaa atatctgaca gttggttgtc caatgaaatt ctctgccttt
     2521 acgccggata ttaaagctgc gcctctatta ggtgaacata ccgctgcggt attgcaggag
     2581 ctgggttata gcgacgatga aattgctgca atgaagcaaa accacgccat ctgatattaa
     2641 cgggggcttc tggctcccaa tttataaaaa tttcgaggtt attaatcatg tcagatcaac
     2701 ttcaaatgac agatggtatg catatcatcg ttgaagcatt aaaacagaat aatattgaca
     2761 ctatttatgg tgttgtaggt attcctgtga cggatatggc gcgccatgcc caggcggaag
     2821 gcattcgtta tattggtttt cgtcatgagc agtcggcagg ctatgccgct gcggcaagcg
     2881 gttttcttac ccaaaaacca gggatctgcc tgacagtgtc tgcgccagga ttcctcaatg
     2941 gtttgaccgc attggccaac gcaacggtaa atggttttcc gatgatcatg attagcggct
     3001 ccagcgaccg cgcgatcgtc gacctacagc aaggtgatta tgaagagctg gaccaaatga
     3061 atgcggcaaa accgtatgcc aaagcagcat ttcgcgttaa tcagccgcag gatcttggca
     3121 ttgcattggc acgcgctatc cgggtctcag tatcgggtcg ccctggcgga gtttatcttg
     3181 atttgccagc aaatgtcctg gccgcgacga tggaaaaaga cgaagcgtta accacgattg
     3241 taaaagttga aaatccgtcg ccagcattat tgccatgccc gaagtcagtc actagcgcaa
     3301 tttcgctttt agcaaaagct gaacggccat taattatcct tggcaaaggc gcggcgtatt
     3361 cacaagctga cgaacagctt cgtgaattta ttgaaagtgc ccagattcca ttcctgccaa
     3421 tgtctatggc gaaagggatc ctcgaagata cgcatccact ttctgcggca gctgcgcgtt
     3481 cgtttgccct ggcaaatgct gacgttgtca tgcttgttgg tgcacgactg aattggttac
     3541 tggcacacgg taaaaaagga tgggcggcag atacacagtt tattcaactg gatattgaac
     3601 cgcaggaaat tgacagcaac cgccccattg ctgtgccagt cgttggcgat attgcatcca
     3661 gtatgcaagg tatgctggca gagctgaaac aaaacacatt tacgactcca ctggtatggc
     3721 gcgatatttt aaatatccac aagcagcaaa atgcacaaaa aatgcatgaa aaattaagta
     3781 cagataccca accattaaat tactttaatg cattaagtgc tgtgcgcgat gtattgcgtg
     3841 agaaccagga tatttattta gttaatgaag gtgcaaatac cctggataat gcacgaaata
     3901 ttattgatat gtataaacca cgtcgtcgtc tggattgtgg tacctggggt gtcatgggca
     3961 tcggtatggg ctatgccatc ggtgctagcg tgacctctgg ttctccggtt gtcgccattg
     4021 aaggtgatag tgcttttggt ttcagtggga tggaaattga aacgatttgt cgatataacc
     4081 tgccggtaac gatcgttatt tttaataatg gcggcatcta cagaggagac ggtgttgatc
     4141 tcagtggcgc tggtgcacca tcaccaacag atctgttgca ccatgcaagg tatgacaaat
     4201 taatggatgc gtttcgtggc gttggctata acgtcaccac gacagatgaa cttcgtcatg
     4261 ctttaaccac cggtattcag tcgcgcaaac cgaccattat taatgtggtc atcgaccctg
     4321 cagcaggaac tgaaagtggc catattacca aacttaaccc aaaacaagtc gctggtaatt
     4381 aaatactgac atcgtctcct cttgccaact cattatgcga tggtgagggg acccttatcg
     4441 gaaatactat tatgctaaca ttttttattg gcgatttatt gcctattatc gtaatcatgc
     4501 tgttgggtta ttttagcggc agacgagaaa ctttttcaga agatcaagcc cgggcattta
     4561 ataaactggt attaaactac gcgcttcctg cagccctatt tgtttctatt acccgggcaa
     4621 acagggaaat gatttttgcg gacactcgtc tgacccttgt atcactcgtg gttattgtcg
     4681 gatgtttctt tttctcctgg tttggttgct acaaattttt taaacgtacc catgcagaag
     4741 cagctgtatg tgcattaatt gcaggttcac ctaccattgg attcctgggg tttgcagttc
     4801 tcgatcctat ttatggtgat tccgtatcaa caggtttagt ggtagcaatt atttctatta
     4861 ttgttaacgc aattactatt cctattggtc tgtatttgct gaatccttcc tcaggagcgg
     4921 atggtaagaa gaatagtaat ctgagcgcat taatttctgc ggcaaaggag ccagttgtat
     4981 gggcacctgt tctggcaacg atcctggtgt tggttggggt aaaaattccg gcagcatggg
     5041 acccaacctt taatctgatt gcgaaggcta actcaggggt agcggtattc gctgcggggt
     5101 tgactctggc tgcacataaa ttcgagttca gtgccgaaat tgcttataac accttcctga
     5161 agctgattct gatgccactg gcgctgcttc ttgttggtat ggcatgtcat ttgaacagcg
     5221 aacatctgca gatgatggta ctggcaggcg cattaccgcc ggcattctcc ggaatcatta
     5281 ttgccagccg gtttaatgtc tacacccgca ctggtacagc gtcattggct gtgagcgtac
     5341 tgggttttgt cgtcacggct cccttgtgga tttatgtcag tcgactggtt tcataattaa
     5401 tcatgttatt gattttacat aaatgccgaa gtgtcttttc accttcggct tttttcaagg
     5461 cataagcaaa tgacaaataa tgaaagcaaa gggccgtttg aaggcttatt agttatcgat
     5521 atgacacatg tccttaatgg acctttcgga actcaacttc tttgtaatat gggcgcaagg
     5581 gtaattaaag ttgagccgcc gggtcatggt gatgataccc gcacatttgg tccctatgtg
     5641 gatggacact ctattacagt tttattaatc atggtaaaga gagtgtggtt cttgatttaa
     5701 agaatgatca cgataaaagt atatttataa atatgcttaa acaagctgat gtattagctg
     5761 agaattttcg cccaggtaca atggaaaaac tggggctttc atgggaaagg ttacaagaaa
     5821 tcaacccgcg cctcatatat gcttcatcgt caggtttcgg acataccggt ccgctaaaag
     5881 atgctcctgc ctacgatacc atcattcagg caatgagcgg gataatgatg gaaacagggt
     5941 accctgatgc tccgccagtg cgtgttggta cctctcttgc ggatctatgc ggcggtgttt
     6001 atttattcag cggaatagtg agtgcacttt atggccgcga aaagagccaa agaggggcgc
     6061 atgtcgatat agcgatgttt gatgccacgc tgagttttct ggagcatggt ctgatggcat
     6121 atattgcgac agggaagtca ccacaacgcc tgggaaatcg ccacccctac atggcacctt
     6181 ttgatgtttt cgatactcag gataagccga ttacgatttg ttgtggtaat gacaagcttt
     6241 tctctgcgtt atgccaggca ctggagctta cggaactggt taatgatccc agatttagca
     6301 gcaatatttt acgcgtacaa aaccagacta ttcttaaaca atatattgag cggacgttaa
     6361 aaacacaggc agctgaagtt tggttagcca gaatacatga agttggtgtg cccgtcgcgc
     6421 cgttattaag tgtggctgag gccattaatt tgccacaaac tcaggcgaga aatatgttga
     6481 ttgaagccgg gggaataatg atgccgggta atccgataaa aatcagtggc tgcgcggacc
     6541 cgcatgttat gccgggagcg gcaacgctcg accagcatgg ggagcaaatt cgccaggagt
     6601 tctcatcata aatgtaagat gtgctggtaa atagctccca catttgaaca ttgtgggagc
     6661 cactatttag ttattttgtt gacagaaaac agcaatttcc tggtctagct ctgcaatgtg
     6721 ttcttttaca gagtttagca actgaagaat ttcaggctta ctgtcatctg aaacaggtgt
     6781 tatttctaac tgatggctaa tattaatcaa cttttgcaaa ttcaggatgt tagccgcacc
     6841 gtggatgcga tgaatacact gatggaaagt tctattatcg ccagcttcta gtgcatgaaa
     6901 cgcagcgggt aaatctttat gtgtttcatg ctggaaagtc atgagaatct cctgcatcag
     6961 ttgtagatcg ttcgccgtat tatttttcag ggcctcgata tcaaggtggc gatactgagg
     7021 tacaatatgc gcaacctggt gtaactgact taaatgtgtt ttcagtacat ccagggtcaa
     7081 cggtttgaac aaacataagt tcatgccgca atttaaccct ttttcacgtt cgttagcctg
     7141 tgcgttggct gtaagccccc agatgggtaa ggacgaattt tgctcacgga gtttgcgagt
     7201 caactcaaaa ccatccacat tcggcatatt aacgtcagta ataagcagat cataatgttg
     7261 catactgact ttgtgtagcg cttgcacacc atcagtggct tcatcaacat catatcctaa
     7321 tagatttagc tggcgtttga gtaatagcct gttggtcgga tgatcatccg cgattaatat
     7381 gctcaacttt tcaggtagtg tgatgggttg ttctgctttt gcctcgacag ccgccacttg
     7441 ttggataatt tctaccggga ttgtgatcgt aaatgttgtt cctatgcctg gatgactttc
     7501 taatgacaaa tcaccctgca tgtttttaat taattctttg cagatcatta agcctaaacc
     7561 agaacctgtt tgctgacgac ctgcacttgt ttggctataa cgtttaaaca gttgttgttg
     7621 ttcttcctgt gataatccac ttccagaatc cataatcgtc attttgatta cagcgtggtt
     7681 gtcatcaatg tgaaccaggg aggtcgtaat ttttactgcc ccctcggtgg taaatttgag
     7741 agcattactc agtaaatttg ataagacctg cttaaacgcc tgagggtcga tcttaaccag
     7801 gtaatgatca ggaaatgtac tgctgcaact taatgcgatc gatttgcttg cagcaatcgc
     7861 accgaaagag tgacaagtgt tctggactaa agtagggata tcgacccatt gtggttgaag
     7921 ttgatagtta cccgattcaa ttttgtcgac atcaaggatt tcaccaatta agccgaggag
     7981 tgattgtccg gtggcgtagg ctagtgaaat cgcctccacc cgttgctcct tgctaagacc
     8041 agaacccgac agaagttcca ggaagcccat aatggagctt attggtgttc ttatttcgtg
     8101 actcattgtt gccagaaact gactttttgc gacagttgca ttgatcgctt tatttctctc
     8161 tacttcgagt gcatgaatta aatcacgcgt ctcggtaata tcttgccaac cacaaatata
     8221 aacagcatgt tcgctggctg gcagattaca taatgtatgc cagtgattaa tgcatctttt
     8281 ctcgatgcca ttatcaattt caaatacctg tgtgtatatt gttcggtttt ctttcgtctc
     8341 tgctgtgact tcatgcgtat tagaaaaaac atctttaaag ggtgattcac tgttttctaa
     8401 tggcaacatt gcatttttgt agtaatcagc agtgaaataa tgttcaaatg cactattgtg
     8461 actaatgaca ttaccttgcc agtttacaac ataagttgga ttcggtaagg agtccgagag
     8521 cgctttccgg aatgatattt ggttttctaa atcaccctga atgactttac gacgacgaac
     8581 tgagcgtaac aggtagaatc cccataaaag gctactgcca actaataaaa cggataatgt
     8641 cgtaacaata taaaattgct cgctatagag gtcccatgtg tcaatggtca cattgggcat
     8701 tttaatccat ttttcggtca ggcgcagaac ttcgcttggg gggattgcat tcagtgcttt
     8761 attaataata tccttaagtt ccggttctcc gcgaggaaaa gcgaacgaaa gcgatgcatt
     8821 cggaacgccg ggaataagaa aatgatacag ttcattagga tagtaatggt cgatcatgta
     8881 acgtgaattt aactgtgtcg cgaccagagc atcaagttcg ccttccttga ccttgtgaaa
     8941 tgcagcgctg gcgttatcga cttttatcca ctctacctcc ggatacatct cttttaattg
     9001 cgaatgaagc tcataataat atggaatggc aactttcatt ccttttttta atgtttgttc
     9061 actgtcaggc gctttttgca tgacaaaaac gtaaggcgtt gttatgaagg cttcagcaaa
     9121 taaaacatta ttttctctat cttcactata aatagcaccg ggtaatatat cccatccgcc
     9181 gggattcagc tgtgttccag catggatatt gtgtgaaacg gtgatcggag aaaaatttaa
     9241 acctgtttgc aaggtaataa tattaagaat gtcccccata acgccccgaa ccgagccatt
     9301 ttcatccgtc atagaatagg ggggcgagta aggattttcc agcaccttta aatcgggatg
     9361 ctgcttaatc cactgttttt catgttcagt gagttctaat ggcttgttca gaaaggccag
     9421 gtttcctgta tcaagccaat tttgtgatac ttcatagcga acttcatttg ttaaagcatc
     9481 aacaaatcga ttgagtactt cattaagaat gacagattct tttctggtta agaagaagtt
     9541 atattgacgc ggtgagttat aatatttcac tacatttaag gagtgagtga aatagcggga
     9601 a
//
LOCUS       DABSYT020000139         9380 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.139, whole genome shotgun sequence.
ACCESSION   DABSYT020000139 DABSYT020000000
VERSION     DABSYT020000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.139"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(249..1442)
                     /gene="tyrB"
                     /locus_tag="ID725_RS21785"
                     /old_locus_tag="pgaptmp_004357"
     CDS             complement(249..1442)
                     /gene="tyrB"
                     /locus_tag="ID725_RS21785"
                     /old_locus_tag="pgaptmp_004357"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGV7852822.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1695..2774)
                     /gene="alr"
                     /locus_tag="ID725_RS21790"
                     /old_locus_tag="pgaptmp_004358"
     CDS             complement(1695..2774)
                     /gene="alr"
                     /locus_tag="ID725_RS21790"
                     /old_locus_tag="pgaptmp_004358"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGV7852823.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2827..4242)
                     /gene="dnaB"
                     /locus_tag="ID725_RS21795"
                     /old_locus_tag="pgaptmp_004359"
     CDS             complement(2827..4242)
                     /gene="dnaB"
                     /locus_tag="ID725_RS21795"
                     /old_locus_tag="pgaptmp_004359"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGV7852824.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4325..5308
                     /gene="qorA"
                     /locus_tag="ID725_RS21800"
                     /old_locus_tag="pgaptmp_004360"
     CDS             4325..5308
                     /gene="qorA"
                     /locus_tag="ID725_RS21800"
                     /old_locus_tag="pgaptmp_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGV7852825.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5474..5716)
                     /gene="pspG"
                     /locus_tag="ID725_RS21805"
                     /old_locus_tag="pgaptmp_004361"
     CDS             complement(5474..5716)
                     /gene="pspG"
                     /locus_tag="ID725_RS21805"
                     /old_locus_tag="pgaptmp_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGV7852826.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5850..6887)
                     /gene="dusA"
                     /locus_tag="ID725_RS21810"
                     /old_locus_tag="pgaptmp_004362"
     CDS             complement(5850..6887)
                     /gene="dusA"
                     /locus_tag="ID725_RS21810"
                     /old_locus_tag="pgaptmp_004362"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGV7852827.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     LHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            6976..8073
                     /locus_tag="ID725_RS21815"
                     /old_locus_tag="pgaptmp_004363"
     CDS             6976..8073
                     /locus_tag="ID725_RS21815"
                     /old_locus_tag="pgaptmp_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7852828.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            8135..8383
                     /locus_tag="ID725_RS21820"
                     /old_locus_tag="pgaptmp_004364"
     CDS             8135..8383
                     /locus_tag="ID725_RS21820"
                     /old_locus_tag="pgaptmp_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGV7852829.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESSDEWFVH"
     gene            complement(8394..8513)
                     /locus_tag="ID725_RS21825"
                     /old_locus_tag="pgaptmp_004365"
                     /pseudo
     CDS             complement(8394..8513)
                     /locus_tag="ID725_RS21825"
                     /old_locus_tag="pgaptmp_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(8544..9185)
                     /locus_tag="ID725_RS21830"
                     /old_locus_tag="pgaptmp_004366"
     CDS             complement(8544..9185)
                     /locus_tag="ID725_RS21830"
                     /old_locus_tag="pgaptmp_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGV7852830.1"
                     /translation="MPVDLTPYILSGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDLDRGNFVLKN"
     gene            complement(9267..>9380)
                     /locus_tag="ID725_RS21835"
                     /old_locus_tag="pgaptmp_004367"
     CDS             complement(9267..>9380)
                     /locus_tag="ID725_RS21835"
                     /old_locus_tag="pgaptmp_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGV7852831.1"
                     /translation="VETGGAHPLSREPITESMIMRKDECHFDTKREAFCCK"
BASE COUNT         2548 a         2362 c         2166 g         2304 t
ORIGIN      
        1 gtaacaaacc cgatacttat tattaaattt aataatggtt agatgtgcag aaattattac
       61 tccacacgaa aaaaacaggg aaaggaattc cagataattg ggataaactt aacaataaaa
      121 gaactaacaa cgtttattat ctctcacgta gaacgatggt atcaaaaaaa tcatctataa
      181 agaaagagtg aaagcgacga cagtttaatt tcactgcagg ctgggtaatt ccagcctgct
      241 ttcctgcatt acatcaccgc agcaaacgcc tttgccacac gttgcacatt tgccgtattt
      301 aacccggcga cacacatgcg accgctggcg atgagataga caccaaattc ttcacgtagt
      361 cggtcaacct gagcggcact taaaccggta taactgaaca tgccgcgctg attaagcaga
      421 taatcgaaat tgcgttctgg catctctgtg cttaatacct tcaccagttc ctgacgcatt
      481 gccagaatgc gagtacgcat ctcttctact tccgccagcc agctggcttt caatgcctcg
      541 tcattcagca ctgcagccac cacctgcgca ccaaaattcg gcgggctgga gtagttgcgg
      601 cgaactgttg ctttcaattg ccccagtacg cggcctgcag cttcggcatc ttcacacaga
      661 acagaaagtc cgccgacgcg ctcgccgtaa agggagaaaa ttttcgagaa cgaattgctc
      721 accagagcgg gtaatccagc gctggcaatg gcgcgaatgg cgtaggcatc ctcttccata
      781 ccggcaccaa atccttgata ggcaatatca aggaatggga taagctcgcg ggctttgaga
      841 atttcaatca ccgcatccca ctggtcatta gtgagatcgg cacccgttgg gttgtggcaa
      901 catggatgca gcaacacaat actgcgggca ggtaatgttt tcagcatcgc caacaggtca
      961 ttaaagcgca cgccgttagt cgcttcgtca taccaggggt aagtgcttac ttcgaatcca
     1021 gccccggcga atattgctac gtggttttcc caggtaggat cgctgaccca gacgcctgat
     1081 tccgggaagt agcgtttcag gaaatccgcg cccactttca atgcccctga gccgccaagg
     1141 gtttgaatgg ttgctacgcg ctgttgttgc agtaccggat ggtcggcacc aaacagcagc
     1201 ggcgcaatgg catggcgata gctgttaagc ccttccatcg gtaaataaag cgaagcgcca
     1261 tgaggcaccg cattcaggcg cgcttccgcc tccgccacgg ctttcaattg tggaataatt
     1321 ccgtcttcgt tgtagtacag accgatactt aaattcactt tgtcgctgcg agggtcttct
     1381 ttaaaacgct ccataagcgt aagaatcggg tcgccagcgt aggcgtcaac tttttgaaac
     1441 acgcgatggt tctctaggtt tacgggcagg tggttaaaac acaataaacc ggaagaaggc
     1501 gaagatcgag tggatgttca ggggcgaacg gcaattagca acagagtgag actcatgaca
     1561 aacgtacatc cgccagaggc acgaccttta taagagcatg aaagaatatc aacttattga
     1621 attggtagga ttttattggc cggataaggc attcacgccg catccggcac agacaatcaa
     1681 atattacaga acgattaatc cacgtatttc atcgcgaccc ttgaagtcag gcgcgtaata
     1741 agttcgtaag cgcttacttt cgtcatttca gcgatacgtt ctacgggcaa accttcaccc
     1801 cacaaaatga ccgggtcccc ggctttatcc tgtgcctgtg gacctaagtc tacgcagatc
     1861 atatccatcg ccacgcgccc gacaatcggc acttcgcgac cgttcaccag cactggcgta
     1921 ccggacggcg cggcgcgcgg ataaccatcg ccatagccca tcgcaactac accaagacga
     1981 gtatcacgtt cgcttatcca ggttccacca taaccgacag gctctccgac tttatgctca
     2041 cgcacggcaa tcaggctgga ggttagtgac atcactggct gacagccaaa atcggcaccg
     2101 gtggagcgat cttccagcgg cgagacgcca taaagaatga tgcccgggcg cacccagtca
     2161 aaatgcgact gtggccacag cagaatgcca cccgacgcgg caatggaacg ttgacctggt
     2221 ttgccttcgc aaaaggtatt aaagatagcg agttgtttct cggttgcgcc acattttggt
     2281 tcatccgcgc gcgcaaaatg gctgacgata ttcaccggct gccgaacgtt tttgcactgg
     2341 gtcaggcgat gataaaacgc ctcagcctgt tccggcctta cgcccagacg gtgcataccg
     2401 gtatcgagtt tcatccagac ggtaaccggc tcgtccaggc tagcctcttc cagcgcagcc
     2461 agctgttctt cgttatgcac ggcggtatga aaatgttgcg cagaaatcgt cggcagatct
     2521 ctggcatcaa aaaagccttc gagtaacagt acaggtttgg tgattccccc cgcacgcagt
     2581 cgcagagctt cttcgagacg ggctacgcca aaggcgtcag catcggggag cgttcgcgcg
     2641 gtctcaagaa gaccgtgacc ataagcgttc gctttcacca ccgcaaccat tttactggca
     2701 ggtgccagtt cacgaagacg ttgcaggttg tgtcgcagag cgcggcggtt aatcacaaca
     2761 gttgccgctt gcatttgtgt tccttgataa gtgtttgctt taattaccta attcataaaa
     2821 taattattat tcgtcgtcgt actgcggccc cgcatagttg tcgaagcgcg accattgacc
     2881 gttaaaggtc aggcgtaccg tcccgattgg gccgttacgt tgtttaccga taataatttc
     2941 cgcgatgcct tttaaatcac tgttttcgtg atacacctca tcacgataga taaacatgat
     3001 caagtccgca tcctgctcga tagagccaga ttcacgcagg tcggagttga ccggacgttt
     3061 gtcggcacgt tgttccagag aacggttcaa ctgggacagc gccaccaccg gcacgttcag
     3121 ttctttcgcc agtgctttca gcgagcggga gatttctgca atttccagcg tacggttatc
     3181 ggaaagtgcc ggtacgcgca tcagttgcag gtagtcgatc atgataagcc cgatgccgcc
     3241 gtgttcacgg gcaatacggc gtgcgcggga acgcacttcc gttggcgtca ggccggagga
     3301 gtcatcgata tagatattgc gtttttcgag caaaataccc atggtgccgg aaatgcgcgc
     3361 ccagtcttca tcatcgagct gcccggtacg gattttagtc tggtcaacgc gcgacagcga
     3421 cgccagagaa cgcatcatga tctgttctga tggcatctcc agcgagaaaa taagtaccgg
     3481 tttatcctgc aacatcgccg cgttttcgac gaggttcatc gcaaatgttg ttttacccat
     3541 cgacggacgc gcggcgacga tgatcaaatc cgacggctgc aagccagcgg tttttttgtt
     3601 gagatcatca taaccggtgt ttaccccggt aacgccatcg tgtggctgct gaaataactg
     3661 ctcaatacgc gccacggttg cgtcgagcac atcggcgatg ttcttcggcc cttcgtcttt
     3721 gttcgcacga ctttcggcaa ttttaaagac gcgggattca gccagatcca gcagatcttc
     3781 gctggtacgc ccctgcggat caaaaccagc ttcggcaatc tcattcgcaa ccgagatcat
     3841 ctcacggaca acggcacgtt cacgcacgat gtccgcatag gaactgatgt tcgcagcact
     3901 tggcgtattt tttgacaact ctgccagata agcaaaacca ccgacgctat cgagttgccc
     3961 ctggcgttcc agcgattccg caagggtaat cagatcgata ggactaccgc tttcctgcaa
     4021 acgcgccatt tcagtaaaga tatgacggtg tgggcgggtg taaaaatcgt ctgctaccac
     4081 acgctcggct acatcatccc agcgttcgtt atccagcatt aaaccgccca acaccgactg
     4141 ctccgcttcg atcgagtgcg gaggcacttt cagcccggca acttgtggat cgcgttcgcg
     4201 gggttcagcc tgctgtttgt tgaagggttt atttcctgcc atagtgaatg gagttaccga
     4261 gataaagaat gggtcgaaac tttaccatat gaagcagacc ctgacgatac gttctggagg
     4321 acacatggca acacgaattg aatttcacaa gcacggtggc ccggaagtac ttcaagccgt
     4381 agagttcact cctgccgatc cggcggagaa tgaaatccag gtcgaaaata aagccatcgg
     4441 catcaatttt atcgacacgt atatccgcag cggcctttac ccgccgccat cactacccag
     4501 cggattaggt accgaagcgg caggcatcgt gagtaaagtc ggcagtggtg taaagcatat
     4561 taaggcaggc gatcgtgtag tctatgcgca gtcggcgtta ggcgcttaca gctctgtgca
     4621 taacattaat gcggataaag cggcgattct gcctgcggca atttcttttg agcaagctgc
     4681 agcatctttc ctgaaaggct taacggttta ttatctgctg cgcaaaacct atgaaattaa
     4741 acccgatgaa cagttcctgt tccacgcagc agctggcggc gttggcttga ttgcctgcca
     4801 gtgggcaaaa gccctgggcg cgaaacttat cggcaccgta ggaaccgcgc aaaaagcgca
     4861 gagcgcgcta aaagcgggcg cgtggcaggt tattaactat cgtgaagaga atctggtcga
     4921 gcggttaaaa gagatcaccg gtggtaaaaa agtgcgcgta gtgtacgatt ccgtgggcag
     4981 agacacctgg gaacggtcgc tggattgcct gcaacgccgc ggcttaatgg tgagttttgg
     5041 caactcatca ggtgcggtta ccggtgtgaa cttaggcatt ctcaatcaga aaggctcgtt
     5101 gtatgtgaca cgcccttccc tgcaaggcta tatcaccacg cgggaggaat taaccgaggc
     5161 cagtaatgaa ctgttctctt tgattgccag cggtgtgatt aaggtcgatg tcgccgagca
     5221 gcagaaatat ccgctgaagg atgcgcagcg tgcgcatgag attctggaaa gccgcgcgac
     5281 gcaaggttcc agcctgttga ttccataaaa gaaatagggc ttccacctgg gaagcccttt
     5341 ctttttatag ttcggctgta tgtagggtac agcacgatga atctgttaga ggcgcaatag
     5401 tgacagattt gattatcaat tcctattttg ttctaaggat aaaaccttag gttgtgatca
     5461 tccgcacaat cccttagtaa cgccagcggt cataacgctg atatttcggc acttttggtg
     5521 ctttaatcgc cttaataacc cacaccaccg caatcgccag gagtaaccac ggcagcaact
     5581 taatcatcaa tgccagcata ccgccgagga acataatggc cgtcgccaca accagcgcgg
     5641 cgataatgcc cagcaacgaa acgccggtga ccatcaacat gacaaaaaag ccaatcacaa
     5701 aaagtagttc cagcatgatg ctctcccaaa tatgaaatct cttgcaggca ttacaagaat
     5761 catgccaaaa ataatctatt gatttaacag caaaacgccc cgcgacggtg cgcagggcgt
     5821 ggtgaatttg actacttttt ggtgaaaagt taacgcttat ccgccaccag tttgagcgcg
     5881 tgttccagca cgttaatgtc tgcacccgct ttatgggcat tttcacttaa ataacgccgc
     5941 cactgccgcg cgccaggaat accctggaac aagcccaaca tatgccgggt aatatggcca
     6001 agatacgtcc cctggctgag ttcacgctca atgtacggat acatggcgcg cactaccgcc
     6061 accggatcgg catcggtatc cgaggaacca aagatctctc ggtctaccgc cgccagaata
     6121 cccggattct gatacgcttc gcgcccgacc atcacgccat ccatatgttg caaatgcgct
     6181 ttagcctctt ccagcgactt gataccaccg ttaatcgaca tcgtcagatg cagaaagtca
     6241 cgcttcagtt gatacacacg cggataatcg agcggcggga tttcacggtt ttctttcggg
     6301 cttaaccccg aaagccaggc tttacgtgcg tggatgatga acatctcaca ctcgcctttg
     6361 ccggaaacgg tgttgatgaa atcgcagaga aattcatagc tgtcctggtc atcgatgcca
     6421 atacgcgttt tcaccgtcac cggaatcgac accacatcgc gcatcgcttt cacgcagtcg
     6481 gcaaccagct gcgcattacc catcagacac gcaccaaaca tgccgttctg cacccggtca
     6541 gacgggcagc cgacattcag gttgatctca tcatatccac gcgcttctgc cagcttcgca
     6601 cactgcgcca gcgccgccgg atcgctaccg ccgagttgca acgctaccgg atgttcttct
     6661 tcactgtacg ccaggtaatc acctttaccg tgaataatcg cccctgtagt caccatttcg
     6721 gtatacagca acgtattgcg ggaaagcaga cgcaagaaat agcggcaatg tctgtccgtc
     6781 cagtcgagca ttggtgcaac gctaaaccga gaattccagt aaacaccagt tttttcaggc
     6841 atcacgctgg tttgattaat tttttgtgct tcatgattat cgtgcatttt tgaacatttc
     6901 aggctatttt tctcgcgtta ggttcccgca caggttccca cgttttatgg gaacccgaaa
     6961 taacgaggtc gtgtaatggc gtactataac atagagaaac gactaaaatc cgatggcaca
     7021 ccacgctatc gctgtaatgt gattatcaaa gaaaaaggtg ttatcactta cagggaaagc
     7081 aaaacattcc ctaaacatgc tcatgccaaa acatggggca cacagaaagt gatggaatta
     7141 gatctatatg gcattccatc atcaaatgca gttgacggac ttacagtccg tgacttacta
     7201 cacaaatatt taaatgaccc aaatgccgga ggtaaagcag gccgtactaa aagatatgtg
     7261 ctggaactgc ttatggatag tgacatatcc gcgatcaaac tatctgaact gacagaaaat
     7321 gacgtaattg aacattgcag gctaagaaac aacgctggtg caggcccagc aacagtcagc
     7381 cacgatgtta gttatcttgg cagtgttctg gatgcggcaa aacctgtata cggaatcaat
     7441 tacacatcaa acccggcgaa aagcgctcgt ccatatctac ttaaactcgg tttgattggt
     7501 aaatcaaacc gtcgtaatcg tagaccagca tctgatgaac ttgacatgct cattgaaggc
     7561 cttcaacaac gatctactca taaatgctca aaaattccgt tcgttgatat cctcaaattt
     7621 tctgtgtggt cctgtatgcg aatcggagaa gtatgccggt tacgatggga agatctcgac
     7681 caagaacaaa aatctatact agtaagagat aggaaagatc cacgtaaaaa ggaaggtaac
     7741 catatgaaag ttgccttgct tggggaagcc tgggatatcg tccagcgaca accaaaaaaa
     7801 tcagaattca tttttccata taacagcact tctgttaccg caggatttca gagggtaaga
     7861 agcaaattag gtattaaaga tctgcgatat catgatttgc gtagagaagg ggcaagtcgc
     7921 ttatttgagg ctggttttag tattgaggaa gtcgctcaag ttacagggca tcgttcatta
     7981 aacgtgctat ggcaggtata taccgaactg tatccgaaat ctttacataa tcgttttgaa
     8041 gaactccaaa agagcagaaa caagacctct tgacactgtt tatttataca gataaaaata
     8101 acactgtata caaacacagt atagagggat ttttatgcgt attgaaatct gcatagccaa
     8161 agagaaaatg actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag aattaactcg
     8221 gcgcatcagt aagcgttatg acgatgtaga agtgatcgta aaagccacca gcaatgatgg
     8281 gctttctgtt acacgcactg cagataagga ttctgcaaaa acatttgttc aggagactct
     8341 gaaagatacc tgggaatctt ctgacgagtg gtttgttcac taataaacac gtaaatcggt
     8401 aacggctgga aatcattcaa tactagcact atcgaaagtt caccagccaa ccgcagtacg
     8461 ttcttgcata cgaggtgccg cgttttacat gtcgtaccga gaattaattt cattttacga
     8521 aaagttaacc cgccattgct atcttaattt tttaaaacga agttacctct gtcaaggtca
     8581 aaagcacaat tatctcttcc tacaatcatt gaaactgcga ttgactccct gctcaaagga
     8641 tgagccgccc ttgttttaac aagatgtatt aatgccattt tatcatacaa catacaaaca
     8701 tttgaattca ttgaattcct cataaatacc ccatcttctg gttcgcataa tgtaacaggg
     8761 catgttcgta agctctctgg cacttgcaat tgtgcatgct ccacattaaa tgcacatact
     8821 tcaattttat ctaacaactc ctctaactgg tagttagaaa gcaaatccat attttcgagc
     8881 atctcaattt cacgcagtac tgtaacttca cggcttgtca aactaccatg attaagtgta
     8941 cgttcaatat gttctatata ttgaaggcca atctggatat tatcattcga taaaccatcc
     9001 tggttaagac ttcccataaa atatccattt acctgcatag gtcgtattga caccatcaac
     9061 tcacccagtc ttatttgagc ttctccacga atagtctgat tacgtatctc agacagggtt
     9121 tcttgaggaa tgtcagacaa aaaactaacc ccagataaaa tataaggcgt taaatctaca
     9181 ggcattcctc tccctccatt ttaataactt aattttgctt tagtaaaaaa caaaagcaca
     9241 taaaactata tttatatcat taattatcac ttacaacaaa aagcttctct ttttgtatca
     9301 aagtgacatt cgtcttttct cataatcatt gattctgtta taggttctcg actcagagga
     9361 tgagctccac cagtttcaac
//
LOCUS       DABSYT020000140         9280 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.140, whole genome shotgun sequence.
ACCESSION   DABSYT020000140 DABSYT020000000
VERSION     DABSYT020000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.140"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            187..549
                     /locus_tag="ID725_RS21840"
                     /old_locus_tag="pgaptmp_004368"
     CDS             187..549
                     /locus_tag="ID725_RS21840"
                     /old_locus_tag="pgaptmp_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852832.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(728..1186)
                     /gene="ybbJ"
                     /locus_tag="ID725_RS21845"
                     /old_locus_tag="pgaptmp_004369"
     CDS             complement(728..1186)
                     /gene="ybbJ"
                     /locus_tag="ID725_RS21845"
                     /old_locus_tag="pgaptmp_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGV7852833.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1183..2100)
                     /gene="qmcA"
                     /locus_tag="ID725_RS21850"
                     /old_locus_tag="pgaptmp_004370"
     CDS             complement(1183..2100)
                     /gene="qmcA"
                     /locus_tag="ID725_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGV7852834.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2246..2923
                     /gene="fetA"
                     /locus_tag="ID725_RS21855"
                     /old_locus_tag="pgaptmp_004371"
     CDS             2246..2923
                     /gene="fetA"
                     /locus_tag="ID725_RS21855"
                     /old_locus_tag="pgaptmp_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGV7852835.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2910..3689
                     /gene="fetB"
                     /locus_tag="ID725_RS21860"
                     /old_locus_tag="pgaptmp_004372"
     CDS             2910..3689
                     /gene="fetB"
                     /locus_tag="ID725_RS21860"
                     /old_locus_tag="pgaptmp_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGV7852836.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3752..4606)
                     /gene="cnoX"
                     /locus_tag="ID725_RS21865"
                     /old_locus_tag="pgaptmp_004373"
     CDS             complement(3752..4606)
                     /gene="cnoX"
                     /locus_tag="ID725_RS21865"
                     /old_locus_tag="pgaptmp_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGV7852837.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4667..5476)
                     /gene="ybbO"
                     /locus_tag="ID725_RS21870"
                     /old_locus_tag="pgaptmp_004374"
     CDS             complement(4667..5476)
                     /gene="ybbO"
                     /locus_tag="ID725_RS21870"
                     /old_locus_tag="pgaptmp_004374"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGV7852838.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5466..6089)
                     /gene="tesA"
                     /locus_tag="ID725_RS21875"
                     /old_locus_tag="pgaptmp_004375"
     CDS             complement(5466..6089)
                     /gene="tesA"
                     /locus_tag="ID725_RS21875"
                     /old_locus_tag="pgaptmp_004375"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGV7852839.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6060..6746
                     /gene="ybbA"
                     /locus_tag="ID725_RS21880"
                     /old_locus_tag="pgaptmp_004376"
     CDS             6060..6746
                     /gene="ybbA"
                     /locus_tag="ID725_RS21880"
                     /old_locus_tag="pgaptmp_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGV7852840.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6743..9157
                     /gene="ybbP"
                     /locus_tag="ID725_RS21885"
                     /old_locus_tag="pgaptmp_004377"
     CDS             6743..9157
                     /gene="ybbP"
                     /locus_tag="ID725_RS21885"
                     /old_locus_tag="pgaptmp_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGV7852841.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNYVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2124 a         2377 c         2544 g         2235 t
ORIGIN      
        1 ttatataaat aaaaaaggaa atcatttcgt gctctttcaa tgtaaaaaag agtaaagact
       61 tgttttgata agcgaaataa attctgtttc tggactaaac gtgaagcggt tcctgatgtg
      121 tgctaaaatg cgtttctttg ataaatctca atttttaaga ggtttacgcc aggcaggaaa
      181 atttaaatga atgaatttga gaagattttt aatgaaatga atctggaccg ggcattgcta
      241 ccgatccttt ttcgttcgaa ccggtcgaca gtgtggaagt atttaagcgg cgatagtacg
      301 gctcctgcct ctgccatgtc gctgattatg ctgttgcagt tgatacagaa acgaaacccc
      361 gatctgttag ccgaatggtt aacgctttct gatttcacca ttccgccaga agtttacctg
      421 gaccagcctg attactggaa agggtgggtg tatacgcaac acaaggtcaa taaaaacgtc
      481 ctggaatatc taaaaaaaca ctatccggat gaagaccaaa aaagtatgag taaaggccgg
      541 gaagagtaaa ttgccaatgc cagttaacat ttaacatggc ggaggcggcc tacggttttg
      601 gcgtgatatg acgcgcccgc gtcgcatcag gcgtcggtgc acgactgccg gatgcggcgt
      661 aaacgcctta tccggcctac gattcgggcg cagacttccg ggcatgataa gacgcgtcag
      721 cgtcgcatca ggcgatgacc gcacggatga tcagcgttat cccttctatc gcaatgactt
      781 caacatgcgt acctgcgccg agatcctcgc tggcgctgac aggccatgaa ctgtcaccga
      841 cgcgcatatg accgcgcccg ttgaccagcg gagattccag cacaaaacgt cggccaatca
      901 gctgctgccc gcgctggttt aaatgactgt cgctgtgctt ttgttcgcgc acccgccgcg
      961 acaaccattt ccaccacagc caggcggcga gcagcgtcag gacggcaaac atcacccctt
     1021 gccactccca acccagcggc accagccaga ccaccaggcc agtaatcact gctgccacgc
     1081 cactccacaa caaataacca tttccgccca gcatctcggc tgccagcagc aaaccgccga
     1141 gactgagcca gaaaatatgt ggatgaacga ctattaactc catcatggct gagtccgctt
     1201 gttggcgctg tctttcacca gctcggcaat cccggcaatc gaccccatca ggctgctggc
     1261 ctctaatggc atcatcacta ctttgctgtt actggaggaa ccgatctgct gtaacgcttc
     1321 ggtgtatttc tgcgctacga agtagttcac cgcctgaata tcaccggagg cgatggcttc
     1381 agacaccatt ttggtggcgc gggcttctgc ttcagcggaa cgttcacgcg cttcagcctg
     1441 taaaaacgcc gactgacgtt cgccttccgc tttcaggatt tgcgactgtt tttcaccttc
     1501 ggctttgagg atttccgcct gacggatccc ttccgcttca agaatgtaag cgcgtttggt
     1561 acgttccgct ttcatctgcg cgttcattga agagataagc tctgccggtg ggcgcacgtc
     1621 gcgaatttca atacgggtga ctttaatccc ccacgggttg gtggcctcat cgacaatacg
     1681 cagcaggcgt gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc
     1741 caacacggta cggatgttag tcatggtcag gttgatgatc gccagctcca gattgctgac
     1801 ttcataagcc gcgcgtggcg cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac
     1861 gttggcgtta tctttcgaga taacttcctg ggaagggata tcgagcactt gctccatcat
     1921 attgatcttg cgaccaatgc gatccataaa cggcaccacc agactgagcc ccggctgtaa
     1981 cgttttggta tagcgaccaa aacgttctac cgtccattga tagccctgcg gcacgatttt
     2041 gacgcccgcg ccgacaatga ccagcgcgac aaaaatgaga atcgggataa agataagcat
     2101 cggaaaaacc tcctgttgta ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat
     2161 tatacctgat tactgaaaga gagttccccc ttattcctgc gaaggataaa ctgtttttag
     2221 taaaaatcag aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc agctacaaaa
     2281 cgtaggatat ctggcgggtg atgcgaagat tcttaataac atcaattttt cgctgcgtgc
     2341 tggcgaattt aagttaatta ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat
     2401 agttgcttca ttgatcagcc caaccagcgg aacgttactg tttgaaggtg aggatgtcag
     2461 cacactaaag ccagaaatct accgtcagca agtctcttac tgcgcccaga cgccgacgct
     2521 gtttggcgat acggtatacg ataatctgat ctttccctgg cagatccgta accagcagcc
     2581 tgacccggcc atttttctcg attttcttga acgcttcgcc ttgccggaca gcattttgac
     2641 gaagaatatc gccgagctat ctggtggtga aaaacaacgc atctcattga ttcgtaacct
     2701 gcaatttatg ccgaaggttt tattgctgga tgaaatcacc agtgcgctgg atgaaagtaa
     2761 taaacataac gttaatgaga tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct
     2821 gtgggtgaca cacgataaag acgaaattaa tcatgcggat aaagtgatta cactgcaacc
     2881 gcatgccgga gaaatgcagg aagcacgcta tgaactcgca taatattact aacgaatcat
     2941 tagcactggc attaatgctg gtagtggtgg caatcttaat tagccataaa gaaaaactgg
     3001 cgctggagaa agatattctc tggagcgtcg ggcgcgcgat aattcagctg attattgttg
     3061 gctatgtgct gaagtatatt ttcagcgtgg atgatgccag cctgacatta ttgatggtgt
     3121 tatttatctg ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag
     3181 cttttatctc atcgtttatt gccattacgg tcggggcggg aattaccctg gcggtgctga
     3241 ttctctcagg gtcgattgaa tttatcccga tgcaggtgat ccctatcgcc gggatgattg
     3301 ccggtaacgc catggtagcg gtggggttgt gttacaacaa tttagggcaa cgggtcatta
     3361 gcgaacagca acagattcag gagaaactga gtcttggcgc gacgccgaag caggcttcag
     3421 cgatattgat tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa
     3481 cggttggctt agtgagttta ccaggaatga tgtccgggct gatatttgcc gggattgatc
     3541 cggtgaaggc gattaaatat cagattatgg tgacctttat gctgctctca accgccagct
     3601 tgtcgaccat aattgcctgc tatttaacct atcgtaagtt ttataattcg cgccaccagt
     3661 tggtggtgac gcaattgaag aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg
     3721 cgttgtggtt catgtgccga tcaacggaat atcaatacaa caatgcatac agctggcggc
     3781 gatacttcga cgccagtgca tcacccgtac ccagcgcagc gaggatctcc tggaacgttt
     3841 tacgcgtctg accgtctgcg gcggtgagat ctttacgcag atgcccgaac agcaactcca
     3901 gcgcctcttc attgcgccca acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg
     3961 catcttctgg attctccgcc acctgctgtt gcaactgttg aatttccggc gtatcagccg
     4021 cctgcttcag cagttcgatt tgcgccacca gcccctggta gcgggtgtcc tgatcctgca
     4081 acggaatggt tttcagcacc gcttccgcat cttcagaacg gttcagcgca atcagcgttt
     4141 ctgccagcag caggccgatc tccccgttct gattcgacaa ctgccaggcg tctttcagca
     4201 atggcagggc atcggtgtaa ttgccttcct gcatcagttg catcgcctgc tgcgctttca
     4261 gctcttcttc gcgcggcagc actttatcca gcagggcgcg gatcgcctct tccggttgcg
     4321 gcccctggaa gccatctacc ggttgcccgt tctggaacag atacacggtc ggaatcgcgc
     4381 gcagaccaaa ctgggcggca atcatctgct ccgcgtcgca gtccagcttc gccagaataa
     4441 attgcccgtt gtactgcgcc gcaaggcttt ccagaattgg ggttaactgc aaacagtgct
     4501 ggctacgttc agaccaaaaa tagaacagca ccggagtggt catcgactgt tcaagaacct
     4561 gttgcaggtt agattcgtta atgttgacaa tattttctac ggacatggag tcgctctctg
     4621 ttgtcgatat tttctttgac atgggggctt aagcgcgcgc ttcaactcac ccctgcaata
     4681 ttttgtccat cacgcgcccc ggcagcaggc gcttaagcac cattaccgcc caggtcacca
     4741 gcgttaccgg atagcgcatc ttcggcttct cgctaataaa agcatggcgt actttgtcca
     4801 ccaccgcttc cggtcccaac gtaaagcggg cggcgatgcc gggattttcg actggtttat
     4861 cactttgcgt ctggttgacg ttgtcggtga agcgagtacg aatgggaccg ggttcgatca
     4921 ggctgacttt aattccgctg cggcgcagct ccatgcgcag cgcgtctgac cacgcctcca
     4981 gcgcatattt actggccgcg taagcgccac gacccggtgt ggagattaat cccatcaccg
     5041 atgaggtcat cacaatacgc ccttcaccgt gcggcaacat cgcgggtaac aggcgcatgg
     5101 tgagctggtg tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc gcacggctga
     5161 tggtggaaag ggggccatac atgccgaatc cggcattgtt aaagatccca tacagacaat
     5221 tatcggtcag ggcgatcacc tcgtcggctg cgcgatcaac actttctggt gaatccagat
     5281 cgatcaacac gccggtaaat cccatgctgt tcatgcgctc aacatcatcc ggtttccggc
     5341 aacctgccag cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc aggccaattc
     5401 cactggaaca tccggtaatt aagaccgatt tttgcataac tttacctgtc aggatctccg
     5461 ttgctttatg agtcatgatt tactaaaggc tgcaactgct tcgccatcca gtcggcaata
     5521 aacggctggg cgtcgcggtt gggatgaata ccgtcatcct gcatccattg tggcttgagg
     5581 tagacctctt ccattaaaaa gggcagcagc ggaacatcaa actctttggc gagtttgggg
     5641 taaatggcgc taaaggcttc attataacgg cgaccatagt ttgcaggcag acgtatttgc
     5701 attaacaatg gttcagcgtt ggcggctttg acatcctgca aaatctggcg cagcgtttgc
     5761 tcggtttgct gtggctgaaa accacgcaaa ccgtcattgc cgcccagttc aaccagcacc
     5821 caacgcggct gatgctgttt cagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg
     5881 tcgccgctga tgctggcatt gactaccgac gttttactct gccacttatc attcaacaag
     5941 gcaggccagg ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc
     6001 aataacgtgt ccgctgcggc agcacggaag gttaacagga ccagaaacag gaagggcaaa
     6061 tgccagcgga aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc
     6121 atgaactctc catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac
     6181 tggtgggcga gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggctggatg
     6241 acggcagcag tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag
     6301 cgcgggcaaa gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc
     6361 ctacccttaa cgcgctggaa aacgtcgagc ttccggcgct gctgcgcggt gagagtagcg
     6421 cggaaagtcg taacggagcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg
     6481 atcatcttcc ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct
     6541 ttaacggtcg acctgatgtg ctgtttgccg acgaacccac cggtaacctt gaccgccaga
     6601 cgggcgataa aattgccgac ctgctgtttt ccctcaaccg tgagcatggc accacgttga
     6661 ttatggtgac ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga
     6721 acgggcagtt gcaggaggaa gcatgattgc acgctggttc tggcgcgaat ggcgctcgcc
     6781 gtcgctatta attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg
     6841 caatatcagc gatcgcatgg agaagggctt aagccaacaa agccgggagt ttatggcggg
     6901 cgatcgggcg ttgcgcagtt cgcgcgaagt gccgcaagcg tggctggagg aagcgcaaaa
     6961 gcgcggcctg aaagtcggca agcagctgac tttcgccacc atgacatttg caggcgacac
     7021 accgcagctg gcgaacgtta aagcggtgga tgatatctac ccgatgtatg gcgatctgca
     7081 aaccaatccc cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat
     7141 ggcactgctt aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat
     7201 tgccggagaa gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg
     7261 tctgatgatg aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt
     7321 cacctggcgc tataaattcg gcggcagcga gaaccagctc gacagctatg agaaatggtt
     7381 gttacctcag cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct
     7441 ggggcgatcg atggaacgct cgcaacagtt cctgctgctt tcggcacttc tgaccttgct
     7501 gctggcagtg gcagcagtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct
     7561 ggtggcgatc ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg
     7621 tcagtggttg atggtgctga cgctttcagc cgttaccggt ggggcgatag gcctgttgtt
     7681 cgaaaacgtg ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag
     7741 cctctggcca tggctatggg cgctgggcac catgacggtc atctcgctgc tggtggggct
     7801 acgaccatac cgcttgttgc tggcaacgca gcctttacgc gtattacgta attatgtggt
     7861 tgcgaacgtc tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct
     7921 gctcgccgga ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt
     7981 actggctttg ctgtgcggtg tactgggctg gatgctgctg aatgtacttc gccgcatgac
     8041 gctgaaatcg ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac
     8101 gttaagccag ctttcggcat tttcgctctc ctttatgctg ctggcactgc tactggtgct
     8161 gcgtggcgat ctgctcgacc gctggcagca gcagttaccg ccagaaagcc cgaactactt
     8221 tttaatcaac atcgccacag aacaggttac gccgctaaaa gcgttcctcg cggaacatca
     8281 gatagtcccg gaatcgtttt atccggtggt gcgagcgcgg ctgacggcga ttaacggcaa
     8341 gccgacagaa ggcaatgaag atgaggcgct caaccgcgaa ctcaatctta cctggcaaaa
     8401 tacgcggccc gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ctgatgaagt
     8461 ttcgatggaa gaggggctgg cgaaacgctt aaacgttacc ctcggcgata ccgtgacttt
     8521 tatgggcgat acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga
     8581 aagtctgcgg cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca
     8641 gagctggctt accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg
     8701 ccagttccct accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt
     8761 gctggagcag gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat
     8821 gttgctgttg ctggcacagg tgcaggtggg aatgcgtcag cgtcatcagg agctggtggt
     8881 gtggcgcaca ctcggtgcgg ggaaaaaact gctgcgtacc acgttgtggt gtgagttcgc
     8941 catgcttggg tttgtttccg gcctggtggc cgcaattggt gcggaaacgg cactggcagt
     9001 gttgcaggcg aaagtgtttg attttccgtg ggagccagac tggcgattgt ggattgttct
     9061 gccgtgcagc ggtgcgctgc tgctgtcgct tttcggcggc tggctgggcg cgagactggt
     9121 taagggtaag gcgctgttca ggcagtttgc ggggtgatga gagtaaataa ggggcgttct
     9181 ggttgaatgg aacgccttgt ttaaattaat tctacaatat gtttcattga tgatgttatt
     9241 gaattggtga tttcctatct ttctattgct gatattaatc
//
LOCUS       DABSYT020000141         9244 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.141, whole genome shotgun sequence.
ACCESSION   DABSYT020000141 DABSYT020000000
VERSION     DABSYT020000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.141"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..1541
                     /gene="espL1"
                     /locus_tag="ID725_RS21890"
                     /old_locus_tag="pgaptmp_004378"
     CDS             <1..1541
                     /gene="espL1"
                     /locus_tag="ID725_RS21890"
                     /old_locus_tag="pgaptmp_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HGV7852842.1"
                     /translation="DYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAFFSQS
                     PSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALLRHPP
                     AGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQLNYV
                     VSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPLLPEG
                     VPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEIITAQN
                     SIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPGLYIS
                     MLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHPLCVT
                     RFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTLGAFA
                     KKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSMSFSADEL
                     KTYL"
     gene            1716..1823
                     /gene="yniD"
                     /locus_tag="ID725_RS21895"
                     /old_locus_tag="pgaptmp_004379"
     CDS             1716..1823
                     /gene="yniD"
                     /locus_tag="ID725_RS21895"
                     /old_locus_tag="pgaptmp_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGV7852843.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1876..2634)
                     /gene="ydiY"
                     /locus_tag="ID725_RS21900"
                     /old_locus_tag="pgaptmp_004380"
     CDS             complement(1876..2634)
                     /gene="ydiY"
                     /locus_tag="ID725_RS21900"
                     /old_locus_tag="pgaptmp_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGV7852844.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2921..3850
                     /gene="pfkB"
                     /locus_tag="ID725_RS21905"
                     /old_locus_tag="pgaptmp_004381"
     CDS             2921..3850
                     /gene="pfkB"
                     /locus_tag="ID725_RS21905"
                     /old_locus_tag="pgaptmp_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGV7852845.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3951..4241
                     /gene="ghoS"
                     /locus_tag="ID725_RS21910"
                     /old_locus_tag="pgaptmp_004382"
     CDS             3951..4241
                     /gene="ghoS"
                     /locus_tag="ID725_RS21910"
                     /old_locus_tag="pgaptmp_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGV7852846.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4347..5207
                     /gene="yniA"
                     /locus_tag="ID725_RS21915"
                     /old_locus_tag="pgaptmp_004383"
     CDS             4347..5207
                     /gene="yniA"
                     /locus_tag="ID725_RS21915"
                     /old_locus_tag="pgaptmp_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGV7852847.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5248..5784)
                     /gene="yniB"
                     /locus_tag="ID725_RS21920"
                     /old_locus_tag="pgaptmp_004384"
     CDS             complement(5248..5784)
                     /gene="yniB"
                     /locus_tag="ID725_RS21920"
                     /old_locus_tag="pgaptmp_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGV7852848.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5931..6599
                     /gene="hxpB"
                     /locus_tag="ID725_RS21925"
                     /old_locus_tag="pgaptmp_004385"
     CDS             5931..6599
                     /gene="hxpB"
                     /locus_tag="ID725_RS21925"
                     /old_locus_tag="pgaptmp_004385"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGV7852849.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6762..7352
                     /gene="ydjM"
                     /locus_tag="ID725_RS21930"
                     /old_locus_tag="pgaptmp_004386"
     CDS             6762..7352
                     /gene="ydjM"
                     /locus_tag="ID725_RS21930"
                     /old_locus_tag="pgaptmp_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGV7852850.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7485..8876
                     /gene="tcyP"
                     /locus_tag="ID725_RS21935"
                     /old_locus_tag="pgaptmp_004387"
     CDS             7485..8876
                     /gene="tcyP"
                     /locus_tag="ID725_RS21935"
                     /old_locus_tag="pgaptmp_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGV7852851.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(8880..9244)
                     /gene="ydjO"
                     /locus_tag="ID725_RS21940"
                     /old_locus_tag="pgaptmp_004388"
                     /pseudo
     CDS             complement(8880..9244)
                     /gene="ydjO"
                     /locus_tag="ID725_RS21940"
                     /old_locus_tag="pgaptmp_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
BASE COUNT         2439 a         2185 c         2177 g         2443 t
ORIGIN      
        1 tggattatat tgaacaggaa cagttaactg ccgaaaatcg taaccattgt atgaaactgt
       61 ctaaaaaaat ccatagagaa aaaactatac aaccaacagt aaatctcaat ggtagtgcat
      121 ttttttcgca atctccttct gacgctattt tttgtcgcca tctgtcattg caatacgcac
      181 ttgattcatt gagaaatgga aaaggcaagg tcaacctgat taaacattac tcctccgttg
      241 aatccataca gcagcatgtc cccttagtcc gggacgcgga gttcagagca ttacttcgcc
      301 atcctcctgc agggagtcgc gttatcgcga gtaaggattt tggcttcgct ttagatattt
      361 tcttctgtcg aatgatggca aacaatgtca gtcatatgtc tgcgatttta tatatagaca
      421 atcatacttt gtcagtaagg ctacgaataa agcagtcggc gtatgggcaa ttaaattatg
      481 ttgtgtccgt ttacgacccg aacgatacca acgttgccgt cagaggcacc cacaggacag
      541 cacggggctt tctctcgctt gataagttca tcagttcagg tcccgatgct cagacctggg
      601 ctgataggta tgttcgcaac tgtgcaattg ctattctgcc cctattacct gagggagttc
      661 caggggctat tttcacgggt atcgcgacac gaatgccatt tgcccctata catccatcgg
      721 caatgttgtt aataatggcc acaggccaga ctcaacagct tattacatta ttcagacagt
      781 tgcccatact ccctgaaaaa gaaatcattg aaataataac tgcgcagaat agcattggta
      841 cacctgcttt atttttggct atgatgaacg gacatactga caacgtgaaa atatttatgc
      901 aagaaattca gtcactggta gataatcaca tcattcatga agataatctg gttaaattgt
      961 tgcaaactaa aagtgctaac gaaacacctg gactttatat ctccatgttg tatggattcg
     1021 atgagataat cgatatcttt ctgaatgcat taaccactcc tatagcacaa gagcttttaa
     1081 acaaaaaact ggtgatgagt attttagcca tgaaaataca tgatggtgag ccaggattat
     1141 acgccgcaat ggaaaataat caccctttgt gtgtcacacg gttcctctct aaaattaatg
     1201 gcatcgcctt taaatacaag ttgagcaaag ctaacatcat ggatttatta aaaggcgcta
     1261 cagcacaggg aacccctgca ttatacatcg ctatgagcaa gggtaatgaa gacgtcgtgt
     1321 tatcttatat atcgacgctg ggtgcttttg caaaaaaaca ttcttttagt caacatcagt
     1381 tatttacact gttggctgct aaaaatcatg acaacatgtc agctgttcat atagccattc
     1441 atcataatca ttataaaact gtagaaacat attatgctgc tataaatgta atcagccaaa
     1501 gcatgagttt tagtgctgat gaattaaaga cgtatttata accagctatt tacgatattt
     1561 atatatcgat acataatttt attttttcat aaaacgcaga aacagtgatc aggtgtacaa
     1621 cgccacccga cggctgtaat aacaaaaaat cccttgatgc ctgtcccttt tgttacactc
     1681 cgttatcacg cacaagagat atgcaggaca ctggtatgcc gactaaacgc tttgataaaa
     1741 aacactggaa gatggtggtg gtgctactgg caatctgtgg cgctatgttg ttgctacgtt
     1801 gggcagcaat gatttggggc tgagcagtga caaacgaaga cagccagacc gcataacacg
     1861 gtccggcaga taattttaca tgctgtagcc cagcgataag gttgtccgac ggtcggtatg
     1921 ttccggtgca gactctggag gttcagagtt ccatgtgacg ttgtaagcca ccttcagtcc
     1981 aaaatgctca ttaatagcaa catttaatgc gctttcggag ttcagcgttg tgtcttccgc
     2041 gccaaagacg gaaacaccct gcgtaaattt agcgttgtca gtaagctgcc atgcataagc
     2101 accggaggca tagcccagcg gctgagtttc actggcattg tcggtatatt tgtcgtaacg
     2161 cacacctgga ccgaattcaa agcggaaact gtgtaccggg ccattcaaaa actgacgacc
     2221 ataacccgcg gtcaacacat cgcgctctcg atagccgtta taacggtctg tcagccagct
     2281 tgcctgacca aataaatagt cataatcagt taaattaaaa cggctacgcc cgcccgccgc
     2341 atatttttct gaagaacgct catcattaga agaagtatta ctggcgttcc cccacagcga
     2401 ccaggcagtg gtttgtccat accaggtcat ggtggtgtca gccgtaagtg aggagctttt
     2461 cgtattgcct gattgtgcaa gataccctgc gttcagatta ccttcgaaag gttttttagc
     2521 gctggcagga tcgtccatga cagtaaaaac ggaatcatcg gcagctgcat tcagtgacgc
     2581 aaacatgccc cccgccaaca taacgatggc aggaactgtc ttcaaaagct tcatttatca
     2641 agagtccgta caacaaaaaa agagaccatc gcggtcccgg aaactttctt aaggatcaaa
     2701 gattagcgtc cctggaaagg taacgaatta taaaaaggcg cgaataactt atcaatgtat
     2761 tcttatttca ttttttgaat aagcatgtgg cgaaaacaga tttttattta tatatattta
     2821 tctgcaaaat tttaaataaa gctccaataa atcatattgt taatttcttc actttccgct
     2881 gattcggtgc cagactgaaa tcagcctata ggaggaaatg atggtacgta tctatacgtt
     2941 gacacttgcg ccctctctcg atagcgcaac aattaccccg caaatttatc ccgaaggaaa
     3001 actgcgctgt accgcaccgg tgttcgaacc cgggggcggc ggcatcaacg tcgcccgcgc
     3061 cattgcccat cttggaggca gtgccacagc gatcttcccg gcgggtggcg cgaccggcga
     3121 acacctggtt tcactgttgg cggatgaaaa tgtccccgtc gctactgtag aagccaaaga
     3181 ctggacccgg cagaatttac acgtacatgt ggaagcaagc ggtgagcagt atcgttttgt
     3241 tatgccaggc gcggcattaa atgaagatga gtttcgccag cttgaagagc aagttctgga
     3301 aattgaatcc ggggccatcc tggtcataag cggaagcctg ccgccaggtg tgaagctgga
     3361 aaaattaacc caactgattt ccgctgcgca aaaccaaggg atccgctgca tcatcgacag
     3421 ttctggcgaa gcgttaagtg cagcactggc gattggtaac atcgagttgg ttaagcctaa
     3481 ccaaaaagaa ctcagtgcgc tggtgaatcg cgaactcacc cagccagacg atgtccgcaa
     3541 agccgcgcag gaaatcgtta atagcggcaa ggccaaacgg gttgtcgttt ccctgggtcc
     3601 acaaggagcg ctgggtgttg atagtgaaaa ctgcattcag gtggtgccac caccggtgaa
     3661 aagccagagt accgttggcg ctggtgatag catggtcggc gcgatgacac tgaaactggc
     3721 agaaaatgcc tctcttgaag agatggttcg ttttggcgta gctgcgggga gtgcagccac
     3781 actcaatcag ggaacacgtc tgtgctccca tgacgatacg caaaaaattt acgcttacct
     3841 ttcccgctaa caaaaacatt cccccagcat tgggggaatc atcaccaacc tgtcggcaac
     3901 gcgtttctcc gactatgctc aaaagtcatg tgataacaaa ggggtgaact atggccagtg
     3961 gcgatcttgt ccgttatgtc ataaccgtca tgttgcatga ggatacattg actgaaatta
     4021 acgagttgaa taattacctg actcgcgacg gttttttgct caccatgacg gatgatgagg
     4081 gaaatatcca tgagctgggg actaacactt ttggacttat cagtacccaa agtgaagaag
     4141 aaattagaga actggtttcg gggcttaccc aaagtgcaac cggcaaagat cctgaaatca
     4201 ccatcacgac ctgggaggaa tggaatagca acagaaaata aatggttttt gggcaataat
     4261 cagtctgtgg tgtgcgttag ctcgtgtttt tacaccgcat tcttgcgcta accttatgat
     4321 ctggcagaca acatgggaga gacatcatgt ggcaggcaat cagtcgtctt ttgagcgagc
     4381 agttaggtga aggcgaaatc gaactgcgta atgaactgcc tggcggagaa gtccatgccg
     4441 catggcattt gcgctatgca ggacatgact ttttcgtcaa atgtgatgaa agggaactgc
     4501 ttcccggttt taccgccgaa gccgaccaac tggagttact gtcgcgtagt aaaaccgtca
     4561 ccgtgcctaa ggtttgggca gttggcgctg accgtgacta cagttttctg gtgatggatt
     4621 atctcccacc tcgtccgctg gatgcgcata gcgcatttat tcttggtcag caaattgcgc
     4681 gtttacatca atggagcgac caaccacaat ttggactcga ttttgataac tcgctctcca
     4741 caactccaca gcccaacacc tggcaacgtc gctggtcaac gttttttgct gaacaacgga
     4801 ttggctggca gttggaactg gcagcagaaa aagggatcgc tttcggcaat atcgatgcca
     4861 tcgtcgagca tattcagcag cgtctcgcct cacatcaacc gcagccttct ctgttgcacg
     4921 gcgatttatg gtccggcaac tgtgcgctgg gtccggatgg cccgtacatt ttcgacccgg
     4981 cctgctactg gggtgaccga gagtgcgacc tggcgatgtt accgctgcat actgaacagc
     5041 cgccacaaat ctatgacggc tatcagtcag tatccccgct acctgccgat ttccttgaac
     5101 gtcaaccggt ttaccaactc tacacgctgt taaatcgtgc aagattattt ggcggtcagc
     5161 atttggttat tgctcagcag tcactggata gattattagc tgcgtgatat gggttgaaga
     5221 taatggccgc tccgtgcggc cttttgatta aataaaccca agcagagaaa agaacacata
     5281 acctgcagca atacaaataa ccggcaggat atacagtgag aaaaactgca ggaaaatagt
     5341 atggtgcgga acaacaatac gtgactcaat ttgttcacgc gttagcccct cttccccttt
     5401 ggctttttcc agaatgagtt gatcttcaac accttcccgc aagaagcgcg cctggcgact
     5461 catccgggta ccggaatcct gcaacgccag tccaacaaaa atcaggatga aaatcaccca
     5521 gaacataacg ttcacgccac cattaaaatt gggcgtcggg gagttatacc agaacagatt
     5581 caaaaaaggc gtattcgcct gcatcatatc gatcatgaca tgagtaaagt cgagcatgac
     5641 cgcattaatg ccttcctggt tttcctgccg ggtattcata aatttcagca aagaaatcaa
     5701 tgtagagatc aatgcaggta taaaaatcac ccaacccata atccttttca aaacagcaat
     5761 gcgtccagct tgttgatacg tcatgagttc tccttgatta agacgcgtcg tttcacttag
     5821 tttacctgta gatatccgtt ttcgcccatt ctttaaaggc gatatgatag gcgcttaatc
     5881 ataagcacgg cttaatacct tacacataat gctctaaagg agaggttgta atgtcaaccc
     5941 cgcgtcagat tcttgctgca atttttgata tggatggatt acttatcgac tcagaacctt
     6001 tatgggatcg agccgaactg gatgtgatgg caagcctggg ggtggatatc tcccgtcgta
     6061 acgagctgcc ggacacctta ggtttacgca tcgatatggt ggtcgatctt tggtacgccc
     6121 ggcaaccgtg gaatgggcca agccgtcagg aagtagtaga acgggttatt gcccgtgcca
     6181 tttcactggt tgaagagaca cgtccattat taccaggcgt gcgcgaagcc gttgcgttat
     6241 gcaaagaaca aggtttattg gtgggactgg cctccgcgtc accactacat atgctggaaa
     6301 aagtgttgac catgtttgac ttacgcgaca gtttcgatgc cctcgcctcg gccgaaaaac
     6361 tgccttacag caagccgcat ccgcaagtat atctcgactg cgcagcaaaa ctgggcgttg
     6421 acccactgac ctgcgtagcg ctggaggatt cggtaaatgg catgatcgcc tctaaagcag
     6481 cccgcatgcg ttccatcgtc gttcctgcgc cagaagcgca aaatgatcca cgttttgtat
     6541 tagcaaacgt caaactctca tcgctgacag aactcaccgc aaaagacctt ctcggttaat
     6601 gaccaggggc agtgatcacg ctgcccctgg ttctttatct gaatttgcat tcaactgacg
     6661 gattaatcgt caatttaaga gaaagagtta caccgtcacc acttccgtgc actgtataaa
     6721 aatcctatac tgtacgtatc gacagtttag tgagttttat catgacggcg gaaggtcacc
     6781 ttctcttttc tattgcttgt gcggtatttg ccaaaaatgc cgagctgacg cccgtgctgg
     6841 cacagggtga ctggtggcat attgtccctt ccgcaatcct gacgtgtttg ttaccggaca
     6901 tcgatcaccc aaagtcgttt cttgggcagc gattaaagtg gatatcaaaa ccgatcgccc
     6961 gcgcttttgg gcatcgtggt tttacccaca gtctgctggc ggtatttgcg ctgctggcaa
     7021 ccttttacct taaggttcct gaaagctggt tcattccggc tgatgcgcta caaggaatgg
     7081 tgctgggtta tttaagccac atacttgccg atatgctgac acccgccggt gttcccctgc
     7141 tctggccatg ccgctggcgt ttccgcttgc ctatcctggt tccccaaaag ggtaaccaac
     7201 tggaacgttt tatctgcatg gcattatttg tctggtcggt atggatgcct cattcattac
     7261 ccgaaaacag cgctgttcgt tggtcatcgc aaatgatcaa taccttgcag atccagtttc
     7321 atcggcttat taagcatcag gttgaatact aaaaaggcaa aaatcgcctt tctgtaataa
     7381 gcaattccat ttgaatataa gagccagctc acagttctgt taatcttgcg ccaacactat
     7441 gactgctacg cagtgataga aataataaga tcaggagaac ggggatgaac tttccattaa
     7501 ttgcgaacat cgtggtgttc gttgtactgc tgtttgcgct ggctcagacc cgccataaac
     7561 agtggagtct ggcgaaaaaa gtgctggtgg gtctggtgat gggtgtagtt tttggccttg
     7621 ccctgcatac catttatggt tctgacagcc aggtacttaa agattctgta cagtggttta
     7681 acattgttgg taacggctat gttcaactgc tgcaaatgat cgttatgccg ttagtcttcg
     7741 cctctattct gagcgcggtt gcccgtctgc ataacgcatc tcagttaggc aaaatcagtt
     7801 ttctgaccat tggtacgctt ttgtttacca cgctgattgc ggcgctggtc ggtgtgctgg
     7861 taaccaacct gtttggtttg acggctgaag gtctggttca gggtggtgca gaaactgcac
     7921 gtctgaacgc catcgaaagt aactatgttg gtaaagtctc tgacctgagc gttccgcagc
     7981 tggtcttgtc ctttatcccg aaaaacccgt ttgccgatct gaccggagcc aatccgacgt
     8041 caattatcag cgtggtaatt tttgccgcat tcctcggtgt agctgcgctg aaactgctga
     8101 aggatgatgc gccgaaaggt gaacgcgtct taaccgctat cgataccctg caaagctggg
     8161 tgatgaaact agttcgcctg gtcatgcagt tgacccctta cggcgttctg gcactaatga
     8221 ccaaagtggt tgcaggttct aacctgcaag acatcatcaa actgggaagt ttcgttgtcg
     8281 cgtcctacct cggtctgctg attatgtttg cagtgcatgg cattctgctg ggcattaatg
     8341 gcgtgagtcc gctgaagtac ttccgtaagg tatggcctgt gctgacgttt gcctttacca
     8401 gccgttccag tgctgcgtct atcccactga atgtggaagc acaaacgcgt cgactcggtg
     8461 ttcctgaatc catcgccagt ttcgccgcct ctttcggtgc gaccattggt cagaacggct
     8521 gcgccggttt gtatccggca atgctggcgg tgatggttgc gcctacggtt ggcattaacc
     8581 cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagc tccgcaggcg
     8641 ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct gcgatgggcc
     8701 tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac atgggccgta
     8761 cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag tggctgaagc
     8821 aaaccgataa agccattctg gatagcgaag acgacgccga actggcacac cattaattct
     8881 tatgctggca acggtccgtt ttgtaaaggg gccgttgcct tactttttaa atgttccatc
     8941 gcggcgatgc atactgactc atatttaaac agatcgtcag cgaaaaaatg tccaacttat
     9001 caatacattc ctggctcaat gaatgtgcct gtagcgagaa gcttaaaacc tcatacccga
     9061 gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagtata ccacttccca
     9121 ttcccggaag atttagtgga ttaaaaagaa gattgatatt aagcattgat attatcgaag
     9181 gaaactttag gagactctca tttttttgaa tcgcagcaaa aatgctcccg acaggatgta
     9241 tata
//
LOCUS       DABSYT020000142         9221 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.142, whole genome shotgun sequence.
ACCESSION   DABSYT020000142 DABSYT020000000
VERSION     DABSYT020000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.142"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..234
                     /locus_tag="ID725_RS21945"
                     /old_locus_tag="pgaptmp_004389"
     CDS             <1..234
                     /locus_tag="ID725_RS21945"
                     /old_locus_tag="pgaptmp_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGV7852852.1"
                     /translation="GKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLD
                     HLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            206..2135
                     /locus_tag="ID725_RS21950"
                     /old_locus_tag="pgaptmp_004390"
                     /pseudo
     CDS             206..2135
                     /locus_tag="ID725_RS21950"
                     /old_locus_tag="pgaptmp_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2119..2325
                     /locus_tag="ID725_RS21955"
                     /old_locus_tag="pgaptmp_004391"
     CDS             2119..2325
                     /locus_tag="ID725_RS21955"
                     /old_locus_tag="pgaptmp_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGV7852853.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2322..3914
                     /locus_tag="ID725_RS21960"
                     /old_locus_tag="pgaptmp_004392"
     CDS             2322..3914
                     /locus_tag="ID725_RS21960"
                     /old_locus_tag="pgaptmp_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGV7852854.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3904..5409
                     /locus_tag="ID725_RS21965"
                     /old_locus_tag="pgaptmp_004393"
     CDS             3904..5409
                     /locus_tag="ID725_RS21965"
                     /old_locus_tag="pgaptmp_004393"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGV7852855.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5446..5793
                     /locus_tag="ID725_RS21970"
                     /old_locus_tag="pgaptmp_004394"
     CDS             5446..5793
                     /locus_tag="ID725_RS21970"
                     /old_locus_tag="pgaptmp_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGV7852856.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            5851..6102
                     /locus_tag="ID725_RS21975"
                     /old_locus_tag="pgaptmp_004395"
                     /pseudo
     CDS             5851..6102
                     /locus_tag="ID725_RS21975"
                     /old_locus_tag="pgaptmp_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6099..6839
                     /locus_tag="ID725_RS21980"
                     /old_locus_tag="pgaptmp_004396"
     CDS             6099..6839
                     /locus_tag="ID725_RS21980"
                     /old_locus_tag="pgaptmp_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7852857.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            6853..7284
                     /locus_tag="ID725_RS21985"
                     /old_locus_tag="pgaptmp_004397"
     CDS             6853..7284
                     /locus_tag="ID725_RS21985"
                     /old_locus_tag="pgaptmp_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGV7852858.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            7311..7724
                     /locus_tag="ID725_RS21990"
                     /old_locus_tag="pgaptmp_004398"
     CDS             7311..7724
                     /locus_tag="ID725_RS21990"
                     /old_locus_tag="pgaptmp_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGV7852859.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            7705..>9221
                     /locus_tag="ID725_RS21995"
                     /old_locus_tag="pgaptmp_004399"
     CDS             7705..>9221
                     /locus_tag="ID725_RS21995"
                     /old_locus_tag="pgaptmp_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGV7852860.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRR"
BASE COUNT         2114 a         2258 c         2916 g         1933 t
ORIGIN      
        1 ggaaaactga ttgacaccgg gttctgtatt tttgccctca gtaagctggc aatggcgtta
       61 tccagtacgc ttgattccat ccctttatcc atgcagcgac agtttcctga tttaacaccg
      121 cgccatcttg accatctgaa aacccttatt gctaaggggg caaatcagtg tgcgcgggca
      181 ggggataaat taccggattt acttgatgaa tatatcagag caacaactga ataatatgat
      241 gagcgctgtc acaactgcat tacagcccct gataagggca ttgccggtga cgccagttga
      301 atgggctgat caaaattatt atctgcctaa agaatcttca tatggtgagg gcgaatggaa
      361 aacgctgcca ttccagatcg ccatcatgaa cagtatgggg aatgatcaga tccgcactgt
      421 taatctgatt aaatctgccc gtgttggcta tacaaagatg ttgctggggg gggggcgggt
      481 attttattga gcataaatcc cgaaacagtc tgctttttca gcccacggat tctgccgctg
      541 aagattttat gaagtctcac gtggaggcga cgattcggaa cgtgccatgc ctgaaagacc
      601 tttccccatg gctgggtcgt aaacatcgtg acaatactct cacgctgaaa cgcttttcat
      661 cgggcgtcgg tttctggtgc ctgggcggcg ctgccgccaa aaactaccgt gaaaaatccg
      721 ttgacgtggt ctgctatgac gaactttcct cgttcgagcc ggatgtcgaa aaagagggct
      781 cgccaaccct gctgggggat aagcgtattg aggggtcggt gtggccaaaa tccattcgcg
      841 gctcgacgcc taaaatcaaa ggcacctgcc agatcgaaaa agccgctaac gagtcggcgc
      901 attttatgcg tttttatgtg ccctgcccgc actgtgggga ggcgcagtat ctgaaatttg
      961 gcgatgagtc cacgcctttt gggcttaaat gggagaagga cagccctgaa agtgttttct
     1021 acctctgtga acatcatggc tgcgtgatcc atcagtctga actggaccag agcaacgggc
     1081 ggtggatctg tgaaaacacg ggcatgtgga cccgtgacgg tctgacgttt ttcagcgccc
     1141 ggggtgatga aattccgccg ccgcgctcca tcatgttcca tatctggacg gcgtacagtc
     1201 cgttcaccac ctgggtacag attgtctatg actggctgga tgcactgaaa gatcccaacg
     1261 gcctgaaaac ctttgtgaac accacgctgg gcgagacctg ggaagaggcc gtgggcgaaa
     1321 aactcgatca ccaggtactg atggataagg tggtgcgtta cacggcggcg gtgcctgccc
     1381 gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag atgtatgtct
     1441 ggggatgggc tccgggagag gaagcctttc tggtggataa aatcatcatt atggggcgtc
     1501 ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caacaaaaaa taccgccatg
     1561 cggatggcac cgaaatgact atttcccgtg tctgctggga caccgggggg atcgatggtg
     1621 aaattgttta tcagagatca aaaaaacacg gtgttttccg ggtgctgccg gtaaaaggcg
     1681 catctgtcta tggcaagccg gtgatcacca tgccaaaaac ccgcaatcag cggggcgtgt
     1741 atctgtgtga agtgggaacg gacaccgcaa aagaaattct ctatgcccgt atgaaagccg
     1801 atccctcgcc tgcggatgaa gccacgtcgt atgccatccg ttttcctgat gatccggaga
     1861 ttttttcgca gacagaggcg cagcaactgg tggcggaaga gctggtggag aagtgggaaa
     1921 aaggaaagat gcgtctgctg tgggataaca aaaagcggcg taacgaagcg ctggactgcc
     1981 tggtgtatgc ctacgcggca ttacgtgtgt ccgtgcaacg ctggcagctt gatctggctg
     2041 tactggcaaa atcccgggaa gaagagacga cccggccaac cctgaaagaa ctggcagcga
     2101 agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg tcaggcgcgc
     2161 cttgatttat taaccggtaa acgggtggtg tctgtccaga aagatggtcg cagaattgaa
     2221 tatacggcgg cttctctgga tgagcttaac cgggcgatca atgatgcgga gtcggtactg
     2281 gggacaaccc ggcgtcgccg tcgtccgctg ggagtgaggt tatgaaacga acgcctgtcc
     2341 tgattgatgt gaacggcgtt ccgcttcgtg agagtctcag ctacaacggg ggcggtgcag
     2401 gatttggcgg gcaaatggcg gagtggttgc caccggcgca gagtgccgat gcggccctgc
     2461 tgcccgcgtt gcgtctgggg aatgcccggg cagatgatct ggtgcgcaat aacggaatag
     2521 cggccaatgc ggtggcactg cataaggatc acattgtcgg gcatatgttt cttatcagct
     2581 accgtccgaa ctggcgctgg ctggggatgc gggagaccgc agcaaaaagc tttgtcgatg
     2641 aggtggaggc ggcctggtcg gaatacgccg aagggatgtc tggcgagatc gacgtggaag
     2701 gaaaacgcac gttcacggaa tttatccgtg aaggtgtggg cgttcatgcg tttaacggcg
     2761 aaatctttgt gcagccggtc tgggatacgg aaaccacgca gttattccgt acgcgtttta
     2821 aagccgtgag tccgaaacgg gtggacacgc caggacacgg tatggggaac cgttttctgc
     2881 gggccggggt ggaggtcgat cgatatggcc gtgccgttgc gtaccatatc tgtgaggatg
     2941 attttcctcg ctccgggagt ggacgatggg aacggatccc gcgtgaactt cccaccgggc
     3001 gtccggccat gctgcatatt ttcgagccgg tggaggacgg gcagacccgt ggggccaacc
     3061 agttttacag cgtcatggaa cggctgaaga tgctcgattc cctgcaggca acacagcttc
     3121 agtcggccat tgtgaaagcc atgtatgcag cgacgattga aagtgacctt gataccgaaa
     3181 aggcctttga atatatcgcc ggtgcgccgc aggggcagaa ggataatccg cttattaata
     3241 ttctggagaa gttctccagc tggtatgaca cgaataacgt gacgctgggt ggtgtcaaaa
     3301 ttccgcacct tttccccggg gatgatctga aactacagac tgcgcaggat tcagacaatg
     3361 gattttcggc gcttgaacag gcgctgctgc ggtatatcgc cgccggtctt ggcgtttcct
     3421 acgaacagtt gtcccgtgat tactcgaagg tcagttattc aagtgccagg gcctctgcca
     3481 atgagtcgtg gcgctatttt atggggcggc gaaaatttat tgcggcccgg ctggccacgc
     3541 agatgttttc ctactggctg gaagaggcac ttcttcgggg gattatccgt ccgccacggg
     3601 cgcgttttga tttttatcag gcgcgatcag cctggtcacg ggcagagtgg attggtgccg
     3661 gaagaatggc cattgacggg ctcaaggagg ttcaggaatc ggtgatgcgc attgaggccg
     3721 gactgagcac gtatgagaaa gagccggcgc tgatgggcga ggattatcag gacattttcc
     3781 gccagcaggt cagggaatct gcagagcggc aaaaagccgg actctcacgt ccggtgtgga
     3841 tagcgcaggc gtatcagcag cagatagcgg agagtcgcag gccggaagag gagacaacac
     3901 cccgtgagac gtaatctttc acacattatt gccgcagcat tcaatgaacc gctgcttctg
     3961 gagcccgcct atgcgcgggt tttcttttgc gcgctcgggc gcgagatggg ggcagcaagt
     4021 ctttcggtac cacagcagca ggtacagctt gatgctcccg gaatgctggc tgaaacggac
     4081 gagtacatgg ccggaggtaa acgaccggcc cgtgtttacc gggtggtgaa cggtattgct
     4141 gtactgccgg tgaccggcac gctggtgcac cggctggggg gtatgcggcc attttccgga
     4201 atgacaggct atgacggcat tgtcgcctgt cttcagcagg caatggcgga tagccaggtg
     4261 cggggcgtac tgctggacat tgacagtccg ggcgggcagg ccgccggcgc gtttgactgc
     4321 gctgacatga tttaccgcct ccgtcagcag aagccggtct gggcactgtg caatgacacg
     4381 gcctgttctg cagccatgct gctggcgtcg gcctgctccc gacggctggt tacccagaca
     4441 tcccgtatcg gctccattgg cgtgatgatg agccatgtca gctatgccgg tcatctggcg
     4501 caggccggtg tggatatcac gctgatttac tcaggggcgc acaaggtgga tggcaatcag
     4561 tttgaagcct taccggcaga ggttcgccag aacatgcagc agcgcattga tgcggcgcgc
     4621 cggatgtttg ccgaaaaagt ggccatgttt accggtctgt ctgttgatgc cgtcacggga
     4681 acagaggccg ccgtttttga aggtcagtcc ggcattgatg ccgggctggc ggatgaatta
     4741 gtcaatgcgt cggatgccat cagtgtgatg gccacggcgc tgaacagtaa tgtcagagga
     4801 ggcactatgc cgcaattaac tgcaacggaa gccgccgcgc aggagaacca gcgagtgatg
     4861 gggatcctga catgccagga agcgaaagga cgtgaacagc ttgccacgat gctggcagga
     4921 caacagggca tgagcgttga acaggcccgg gcgattctgg ccgcggcggc accgcagcag
     4981 ccggtggcat ccacgcagag tgaagccgat cgcattatgg cgtgtgaaga agcgaacggt
     5041 cgtgaacaac tggcggcaac gctggcggcg atgccggaga tgacggtgga aaaagcccgc
     5101 ccgatcctgg ctgcttcacc gcaggcggat gccggaccct cactccgtga tcagatcatg
     5161 gcactggatg aggcaaaagg ggctgaggcg caggctgaac agctggctgc ctgcccggga
     5221 atgactgtgg agagcgcccg ggctgtgctg gctgcgggat caggtaaggc agaaccggtc
     5281 tctgcatcca caaccgccct gtttgaacgc atcatggcga accattcacc ggctgcggta
     5341 cagggtggcg tgccacagac gtcagcagac ggtgatgcgg acgtgaaaat gctcatggcc
     5401 atgccatgaa gtcagtgctg accatcaaca ggaggttttt acaatatggt aacgaaaaac
     5461 atcactgaac agcgtgcgga agtacgtatt tttgccggta atgatccggc tcataccgcc
     5521 acaggcagca gcgggatttc ctcggcaaca ccggcactga cgcccctgat gctggatgaa
     5581 gccagcggga aactggtggt ctgggacgga cagaaagccg gtagtgcagt tggcatactg
     5641 gtactgccgc ttgaaggcac agagacggta ctgacctatt acaagtcggg gacctttgcg
     5701 acggaggcaa tccgctggcc tgaaagtgtg gatgaacaca aaaaggcaaa tgcctttgcc
     5761 ggcagtgccc tgagtcacgc ggcgctgccg taacacgtta tcaggccacc gcggtggcct
     5821 gactgatttc tgaatgaaag gaactgattt atgggattgt ttacgacccg ccagttactc
     5881 ggttataccg aacaaaaagt gaaatttcgt gcgctgtttc tggagctgtt tttccgccgt
     5941 acggtgaatt tccataccga agaggtgatg ctggacaaaa ttaccggaaa aacgccggtg
     6001 gcggcctatg tctccccggt tgttgaagga aaagtgctgc gtcatcgcgg tggtgaaacc
     6061 cgcgtgttac gtccgggcta cgtcaagccg aaacacgaat tcccctggag ccggtaaaag
     6121 gagccggtac caccctgtgg gtttataacg gtcagggtga cgcctatgca aacccgttgt
     6181 cagacgatga ctggcagcga ctggctaagg tgaaggatct gacgccgggc gagatgacgg
     6241 cagaatccta cgatgataac tacctggatg atgaagacgc ggactggacc gcgaccgggc
     6301 aggggcagaa atctgcaggt gataccagtt ttacgctggc ctggaaaccg ggagaggaag
     6361 gccagaaagg gcttataggc tggtttgaaa gcggcgatgt ccgggcctat aaaatccgtt
     6421 ttccgaatgg cacggtggat gtgtttcgtg gctgggtcag cagtatcggt aaggccgtga
     6481 cggcgaaaga agtgatcacc cgcacggtga aagtcactaa cgtgggtaaa ccttctgtag
     6541 cggaagaacg cagcaaaatt acgccggtca gtgcgattaa ggtgacgccg acatccggta
     6601 cggtggcaaa agggaaaaca accaccctga cggtttcttt tgagccggaa agtgcaacag
     6661 acaagacgtt cagagcggtt tccgccgatc cgtcgaaagc caccattagt gtgaaagata
     6721 tgacaattac ggtaaacggc gtggcgacag gtaaggtgca gatccctgtg gtgagcggaa
     6781 atggtcagtt cgccgcagtg gctgaagtca ccgttactga agcgggcgct gcagggtaaa
     6841 cggaggtcat acatgtttct gaaaacagaa caatttgaat ataacggtgt gtctgtcacg
     6901 ctttccgaat tgtctgcgct gcagcgtatc gagcatcttg ccctcctgaa acggcgtgca
     6961 gaacaggcag aatccagcgg caacctgcag gtaagcgtgg aagatctcgt cagaaccggc
     7021 gcgtttctgg tggcgatgtc cctgtggcat aaccatccac agaaaacgca gtcaccgtca
     7081 atgaatgagg ccgtgatgaa gatagagcag gaagtgctca ccacctggcc tgccgatgcc
     7141 attgcccggg cggaagacgt ggtgttgtgc ctgtccggga tgagcggggc tgttcgtccg
     7201 gatactgata ttactgaagt ggcgaaaaat aacacgctga ctgatgatga tttttctgcg
     7261 ggaaagtctt cgacggcgag ctgaactttg ccctcagact ggcgcgtgag atggggagac
     7321 ccgactggcg cgccatgctt gccgggatga catccaccga atatgccgac tggcaccgtt
     7381 tttaccgcac gcattatttt caggataccc agctggatat gcatttttcc gggctgacgt
     7441 acgctgtact cagcctgttt ttttgcgatc cggatatgca tccctctgat ttcagtctgc
     7501 ttgtcccccg gcatgaggaa gagcaggtgg agaggccgga tgaggacaaa atgctgatgc
     7561 agaaagcggc aggacttgcc ggaggcgtcc ggttcggtgg ggacggaggg cgcgatattt
     7621 tatcgtctgc ggatgtggcg gatgtcatgg tggatgatgc cgcattaatg atggcttcag
     7681 cggggattcc gggaggtgtg agatatgtcc cagccggttg gtgatcttgt tattgacctg
     7741 agtctggatg ctgtccgttt cgatgagcag atgagccggg taaggcgtca tttttcaggt
     7801 ctggataccg acgtcagaaa aaccgccagt gctgttgaac agggcctgag ccgccaggcg
     7861 ctggctgcac aaaaagccgg gatttccgtc gggcagtata aagcggccat gcgaaccctg
     7921 cccgcacagt ttacggatat cgccacgcag cttgccggtg gtcagaatcc ctggctgatc
     7981 ctgctgcaac agggcggtca ggtgaaggac tccttcggcg ggatgatccc catgttcagg
     8041 gggcttgccg gtgcgatcac cctgccgatg gtcggggtca cctcgctggc ggtggcgaca
     8101 ggtgcgctgg tgtacgcctg gtaccaggga gattccacgc tttcagcgtt taataaaacc
     8161 ctggttcttt ccggtaatca gtccggactg actgccgatc gcatgctgac gctctcaaga
     8221 gccgggcagg cagcagggct gacgtttaac caggcgagag agtcactggc agccctggtg
     8281 aatgccggtg tgcgtggtgg tgaacagttt gatgccatca accagagtgt cgcgcgtttt
     8341 gcttctgcat ccggtgtgga ggtggacaag gttgcagagg ctttcggaaa actgaccacc
     8401 gaccctacgt cggggctgat tgcgatggtg cgccagttcc gtaacgtgac ggcagagcag
     8461 attgcgtatg ttgcgcagct gcagcgttcc ggtgatgagg ccggggcctt acaggcggcg
     8521 aacgatatcg ccacgaaagg ctttgatgag cagacccgtc gcctgaaaga aaacatgggg
     8581 acactggaga cctgggcgga taaaaccggg aaggcattca aatcgatgtg ggatgccatt
     8641 ctggatatcg gtcgtcctga gtcctcagcg gatatgctcg ccagtgcaca gaaggcattt
     8701 gatgaggcgg ataaaaaatg gcagtggtac cagagccgga gccagcgccg gggaaaaacc
     8761 gcctctttcc gggccaacct tcagggcgca tggaatgacc gggaaaatgc ccgtctgggg
     8821 ctggcagcgg ccacgctgca gtcggatatg gaaaaagccg gtgaactggc cgccagggac
     8881 cgggccgaac gggacgcatc acagctgaag tataccggag aggcgcagaa ggcgtatgag
     8941 cgtctgctga cgccgctgga gaaatatacc gcccgtcagg aagaactgaa taaggccctg
     9001 aaagacggga aaatcctgcg ggcggattac aacacgctga tggcggcggc gaaaaaggat
     9061 tatgaatcga cgctgaaaaa gccgaagtcg tcaggagtca aagtgtcagc cggtgagcgt
     9121 caggaagacc aggcgcatgc tgccctgctg gcgcttgaaa ccgagctcag gacgctggaa
     9181 aaacacagcg gtgcgaatga gaaaatcagc cagcagcgtc g
//
LOCUS       DABSYT020000143         9206 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.143, whole genome shotgun sequence.
ACCESSION   DABSYT020000143 DABSYT020000000
VERSION     DABSYT020000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.143"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..241
                     /locus_tag="ID725_RS22000"
                     /old_locus_tag="pgaptmp_004400"
     CDS             <1..241
                     /locus_tag="ID725_RS22000"
                     /old_locus_tag="pgaptmp_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGV7852861.1"
                     /translation="FGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSA
                     FAADKLHVTDITRGATYPVLIDSIAVEVNSTDTAA"
     gene            376..555
                     /locus_tag="ID725_RS22005"
                     /old_locus_tag="pgaptmp_004401"
     CDS             376..555
                     /locus_tag="ID725_RS22005"
                     /old_locus_tag="pgaptmp_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGV7852862.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            596..868
                     /locus_tag="ID725_RS22010"
                     /old_locus_tag="pgaptmp_004402"
     CDS             596..868
                     /locus_tag="ID725_RS22010"
                     /old_locus_tag="pgaptmp_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGV7852863.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            945..1160
                     /locus_tag="ID725_RS22015"
                     /old_locus_tag="pgaptmp_004403"
     CDS             945..1160
                     /locus_tag="ID725_RS22015"
                     /old_locus_tag="pgaptmp_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGV7852864.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            1165..1698
                     /locus_tag="ID725_RS22020"
                     /old_locus_tag="pgaptmp_004404"
     CDS             1165..1698
                     /locus_tag="ID725_RS22020"
                     /old_locus_tag="pgaptmp_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGV7852865.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GVGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALSCWGIDR"
     gene            1972..2541
                     /locus_tag="ID725_RS22025"
                     /old_locus_tag="pgaptmp_004405"
     CDS             1972..2541
                     /locus_tag="ID725_RS22025"
                     /old_locus_tag="pgaptmp_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGV7852866.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            2541..2690
                     /locus_tag="ID725_RS22030"
                     /old_locus_tag="pgaptmp_004406"
     CDS             2541..2690
                     /locus_tag="ID725_RS22030"
                     /old_locus_tag="pgaptmp_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852867.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTL
                     SGLVG"
     gene            2698..3162
                     /locus_tag="ID725_RS22035"
                     /old_locus_tag="pgaptmp_004407"
     CDS             2698..3162
                     /locus_tag="ID725_RS22035"
                     /old_locus_tag="pgaptmp_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGV7852868.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGYRSLRINATCPGP
                     VREATGTARVDNATGPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
     gene            complement(3194..3487)
                     /gene="bor"
                     /locus_tag="ID725_RS22040"
                     /old_locus_tag="pgaptmp_004408"
     CDS             complement(3194..3487)
                     /gene="bor"
                     /locus_tag="ID725_RS22040"
                     /old_locus_tag="pgaptmp_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGV7852869.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGTENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            3637..3840
                     /locus_tag="ID725_RS22045"
                     /old_locus_tag="pgaptmp_004409"
     CDS             3637..3840
                     /locus_tag="ID725_RS22045"
                     /old_locus_tag="pgaptmp_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852870.1"
                     /translation="MSTALTGNFSAGVCGDNQKRCTPGFLIFHEMGAISREAACPVRW
                     WKKPDKTTALCKYRSNMVLLCEI"
     gene            3896..4702
                     /locus_tag="ID725_RS22050"
                     /old_locus_tag="pgaptmp_004410"
     CDS             3896..4702
                     /locus_tag="ID725_RS22050"
                     /old_locus_tag="pgaptmp_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGV7852871.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTNQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            4683..6389
                     /locus_tag="ID725_RS22055"
                     /old_locus_tag="pgaptmp_004411"
     CDS             4683..6389
                     /locus_tag="ID725_RS22055"
                     /old_locus_tag="pgaptmp_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGV7852872.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            6389..8533
                     /locus_tag="ID725_RS22060"
                     /old_locus_tag="pgaptmp_004412"
     CDS             6389..8533
                     /locus_tag="ID725_RS22060"
                     /old_locus_tag="pgaptmp_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGV7852873.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     DTEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQL
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            8691..>9206
                     /locus_tag="ID725_RS22065"
                     /old_locus_tag="pgaptmp_004413"
     CDS             8691..>9206
                     /locus_tag="ID725_RS22065"
                     /old_locus_tag="pgaptmp_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852874.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYL"
BASE COUNT         2472 a         2094 c         2531 g         2109 t
ORIGIN      
        1 ctttggcgca tttgataacg aatggcatac gctggctttc cgctttgccg ggaataacag
       61 cctgcaggtg acgccggtta ttgatggtca ggatggcaca ccgttcacgc tgacgcagtc
      121 accggtcagt gcctttgcgg cggataaact gcatgtgaca gacattacca gaggtgcgac
      181 ttacccggta ctgatagaca gcattgcggt ggaagtgaac agcacagaca ctgcggcatg
      241 ataaaaaaac cgccagcgac aggaatggac gctggcggtg gtgataccta tggagaaaaa
      301 ataaaggaac gatactttcg tactctggtt tttaatgaaa acagttctta ttgtcaacaa
      361 taacggaaag aaattatgac atttctgaac cagttaatgc tgtacttctg tacggtggtc
      421 tgtgtgctgt atctcctttc gggtgggtac agggccatgc gtgacttctg gcgcagacag
      481 attgacaaaa gggccgctga gaaaatcagc gccagtcagt cagccggaag caaacccgaa
      541 gagccgctca tttagcggca actttcttaa tcacaccttt cgacgagaaa atcccatgtc
      601 agaaattaca tccctggtca ctgcagaggc agtgaaggac gtcctgcgct ctgaagaagt
      661 ccggagcgca ctgaaacaga aacttcgcca taacctggaa gcgcgtcttg atgcagaagt
      721 ggatgccatt ctggatgaac tgcttggtgt acaggcagag ccaccgactg aagcgggaga
      781 taccaccgca gagagcggtg aagttcagcc tgaatcaccg gtcgccgatg cgactgaacc
      841 tcaacccgaa tcggtcatga tgctgtaacg gggagtcagg gccatcagta aacagctgct
      901 ggcctttttc atgttgtgag cttccggatt gcgggagacg gggtatgtac cagatggaaa
      961 aaatcacaac aggtgtgtca tacaccacgt cagcggtggg aacgggctac tggttcctgc
     1021 agttgctgga cagggtttcc ccgtctcagt gggcggcaat aggcgtgctg gggagtctgc
     1081 tgtttggtct gctgacgtac ctgacgaacc tgtatttcaa gattaaagaa gaccggcgta
     1141 aggcggcgcg gggagagtaa gctgatgagc aggaaattcc gctatggttt atcagcggta
     1201 gttctggcgc tgattgccgc aggtgcttct gcgcctgaaa tcctcgacca gtttctggat
     1261 gaaaaggaag gtaaccacac cacggcatac cgtgatggtg tgggtatctg gaccatctgc
     1321 cgtggagcca cccgggtgga tggtaagcct gtgattcctg gcatgaagct gtcgaaggaa
     1381 aaatgcgacc gggttaacgc tatcgaacgg gataaggcgc tggcatgggt ggcgaagaac
     1441 atcagagtgc cactgactga accccagaaa gcgggtattg cgtcattctg tccttacaac
     1501 attggccccg gtaagtgttt cccgtcgacg ttttataaac gaattaatgc aggtgatcga
     1561 aaaggtgcct gcgaagcgat tcgctggtgg attaaggacg gtggcagaga ctgccgtatc
     1621 cgctcaaata attgctacgg tcaggtatca agacgtgacc aggagagtgc gctgtcatgc
     1681 tggggtatcg acagataagc agaatatttt gctgaaaaat gacgttggcc aacgcggcag
     1741 ataacacgaa atcctgcaaa ctggcaaaat gtaagtgaat aaagttaaca agattgtttc
     1801 atgaagaggc accgtaatgg tgcctttgtc atttctgcgc ttcgcacaag cgtaaataaa
     1861 ccaaagaacc tttcaggatg agccctggtg gataaccggc agtggtctgg ttaaccctct
     1921 ttgggctggt tattcctgtg cgcagggttc atcactaaaa ggaaataacc gatgaatatg
     1981 atgaccgtgc cgtttcacgg cgattctctt tatgtggtta accataacgg cgaaccatac
     2041 gttcccatga aacctgtcgt tgcggggatg gggctggcct ggcaatcaca gttggctaag
     2101 ttaagacagc gttttgcgtc aactataacg gaaatcgtta tggttgctga ggatgggaaa
     2161 cgacgcaata tggtgtccct gccgcttcga aaacttgcag gctggttaca aaccatcaat
     2221 cccaacaaag taaaacccga aatccgcggc aaggtaatcc agtatcagga agagtgtgac
     2281 gatgttctct atgaatactg gacgaagggt tttgtcgtta atccccgtcg aatgagtgtg
     2341 atggaagaac tcaatcaggc ttgcgctgac atgaaacggg ataaaaacat tgccagtgtg
     2401 tttgctaccg ggctgaatga gtggaaacag gttaaatccg cgcatgtatc aaaaatccgc
     2461 acattgataa acgaagcgaa tctgctgatt gattttgtcc tggctgatac aggcaaaggg
     2521 aaaataacaa aggcggattg atggagtggt ggctaatgat atcggataaa ctcataacgc
     2581 tggcgaagat cctctgtgta atcgtcggca tttcattttt agtcatgctg gttgccattt
     2641 tcttttccac cgcctggcga gtcctgacgt tatcgggact ggtggggtga aagagagatg
     2701 aaccgtgttc tgtgtgtggt gattattgtc ctgctggtag cctgtggtgc gcttagtctg
     2761 gggctgaatc attaccgcga taacgccata acctacaaag agcagcgcga taaaaaagtc
     2821 agtgagctgg agctggcaaa tgcaaccatt actgatatgc agcagcgcca gcgtgatgtt
     2881 gctgcacttg atgccagata ctcgagggaa ttagccgatg cgagagctga aaatgaaact
     2941 cttcgcgctg acgttgccgc tggttaccgc agcctgcgga tcaacgccac ctgtccaggt
     3001 cccgtgcgtg aagccaccgg caccgcccgc gtggataatg caaccggccc ccgactggca
     3061 gacaccgctg aacgggatta tttcaccctc agagagaggc tgatcactat gcaaaaacaa
     3121 ctggaaggaa cccagaagta tattaatgag cagtgcagat agagttgccc atatcgatgg
     3181 gcaactcatg caattattgt gagcaataca cccgcgcttc cagcggagta taaatgccta
     3241 aagtaataaa accgagcaat ccatttacga atgtttgctg ggtttctgtt ttaacaacat
     3301 tttctgtgcc gccacaaatt ttggctgcat cgacagtttt cttctgccca attccagaaa
     3361 cgaagaaatg atgggtgatg gtttcctttg gtgctactgc tgtctgtttg ttttgaacag
     3421 taaacgtctg ttgagcacat cctgtaataa gcagggccag cgcagtagcg agtagcattt
     3481 ttttcatggt gttattcccg atgctttttg aagttcgcag aatcgtatgt gtagaaaatt
     3541 aaacaaaccc taaacaatga gttgaaattt catattgtta atatttatta atgtatgtca
     3601 ggtgcgatga atcgtcattg tattcccgga ttaactatgt ccacagccct gacggggaac
     3661 ttctctgcgg gagtgtgcgg ggataatcaa aaacgatgca caccgggttt tctcattttt
     3721 cacgagatgg gagcgatttc ccgcgaagcc gcctgtccgg tgcggtggtg gaagaaaccg
     3781 gataaaacaa ccgcattgtg caaatatcga tcaaatatgg tgctgctgtg tgaaatctga
     3841 aaaatcacag cggtcattat gcatcagttt ttaacacagg acgtcagaac gtgacatggc
     3901 aaagctggac tggaaaaagc tggagcaggc attccgacgc gaacatgccg aaacgggaat
     3961 aacattactg gactggtgcc ggaagaaaaa gattaattac aacaccgcca gaacccgtat
     4021 aaaaatgggc aaaatcgatc atgaaattga tcataaaacc gatcatgaaa tcgatcatga
     4081 catctcagat gaagaaccct gcaatgacgc gggttccggc gatgaaaaat gcgcaaaaaa
     4141 ctctgaaaaa aactgcgcaa attcggcaga aacgaaacgg attcgtggtt cccgactttt
     4201 accaccttca aacgcttttt ctcagcgaaa cacccacgcc gtaagacacc gtggatatgc
     4261 gaagtatctt gaggcagata acctcatgga tgatgcgtcc gacatggtgc tgttcgatga
     4321 actggtgttc acccgggccc gcgcactttc agtaactaag gcacttaaag ggatgttcgc
     4381 cgacctggaa gaggcaactg acgtggaaac ccgtgttgct ctttacgaca aaatactcaa
     4441 agctgaacag gcccttgacc ggaatattgc ccgtatcgag tcaattgaac gctcattgct
     4501 gacgctggac gtcctggctg agacagcacc aaaacttcgt gctgaccggg aaagaatcaa
     4561 cgccgccaga gataaactca gagctgaaac cgatattctg accaaccagc gtcggggcgt
     4621 tgttacgcct gtcagtgaca tcgtgtcatc gctgcatgaa atgagtaatt cggggagact
     4681 ggatgacatt ccggaagaat gaaccgcgat gtgatgagcc gtcagaaatg accgaggctg
     4741 aacaacgtct gttcatcatg actaaactga gcaatccctg gtggcggctc aatcatctct
     4801 acaaaataca gaacgaaaaa ggtgaactgg tcaccttcag aatgcgaccg gcgcagcgcc
     4861 agttgttccg gagcatgcac aataaaaata ttatcctgaa agcgcgccag ctgggatttt
     4921 ccacagccat tgatatttat cttctcgacc aggcattatt cattccgcat ctcaaatgcg
     4981 ggatcgtcgc tcaggataaa caggctgcca gtgaaatttt ccgcacaaaa attgctgtac
     5041 cgtttgatca tctccctgac tggctgagag cctcattcac catcgttgaa cgtcgtagcg
     5101 gtgccagcgg tggctatatc ctgtttggtc acggctcgag tatccaggtg gcaacctcat
     5161 tccgttcagg tacggtgcag cgcctgcata tctcagagca cggcaaaatt tgcgcgaaat
     5221 atccggctaa ggcgaaagaa ctgcgaaccg gtacgcttaa tgccgtctct gatgaatgca
     5281 ttatttttga tgagtccact gctgaaggcg tgggtggtga tttttacgag atgagtaacc
     5341 gagcacagga gatcactgca tcaggcttat tgctgacggc acaggattat aaattccatt
     5401 tttacgcctg gtggcaggat cctaaataca gcgccagagt gccggaaagc gggctgaagc
     5461 tgtcacggga aaaaatgacg tatttttctg cggttgagaa ggcaatgaac atcacgctta
     5521 ctgatgaaca gaagcagtgg tacatcaata aggaaactga acagcgtgag gaaatgaagc
     5581 aggagtttcc ctcaacgcca caggaggcgt ttctgacgtc cggacgacgt gtgttcagtg
     5641 ccgaaagtac gttgcaggca gaatcattct gttcgccacc gatgattgtt tatgacattg
     5701 aacctgttac aggagcgaag actaaagctc agtctctgcg tgaaggaaat aaaaacgagt
     5761 tgcagcggac gctgatgaat tatctgctgg tatgggaact gccggatccg gatgaagagt
     5821 atgtttgtgg ggcagatact gccgaagggc tggagcacgg agaccgctca tcgctggatg
     5881 ttgtcaaacg cagtaatggc gagcaggtgg ctcactggtt cgggcatctc gatgctgaac
     5941 tttttgctca tctcatttcg caggtctgtc gtatgtataa caacgcgttt gtggggccgg
     6001 agcgtaataa tcacggacat gcagttatcc tgaaactccg ggaactctat ccgacacgtt
     6061 atatctacaa cgaacagcat cttgaccagg catatgacga cgatacgccc cgccttggct
     6121 ggctgacaac ccgtcagagc aaacctgttc tgaccgaagg aatgaaaacg cttctgaata
     6181 atggaatatc agggatccgc tggtcaggca cattatcgga aatgaacacc tacgtttatg
     6241 acgcgaaagg ctccatgaat gcacaggaag gctgctttga tgatcagctc atgagctaca
     6301 tgattgccca ggagatgcgc gccagaatgc cggtgagggt aaaacagaaa acggataaac
     6361 gcagaaccac acactggatg gcacactgat gaaaaatgaa attaacacca cagcgatgaa
     6421 aaacgatcat ggatccacgc cgcgtttttc tcagcgtcag ttactgtctc tctgttctga
     6481 tattgacagt cagcctctct ggcgtgatgc cgcaaacaag gcctgtgcgt attatgatgg
     6541 cgaccagctg gcaccggaag ttatccaggt actgaaagat cgcggtcagc ccatgaccat
     6601 ccataacctc attgccccca cggtagatgg tgtactggga atggaggcaa aaacaagaac
     6661 ggacctgata gtgatgtcag acgatccgaa cgatgataca gagaaactgg cagaggccat
     6721 taatgcggag tttgctgatg cgtgccgtct tggcaacatg aataaggccc gctccgatgc
     6781 ctatgcggaa caaatcaagg cgggcctcag ttgggtggag gtcagacgaa acagtgatcc
     6841 gttcgggcct gaatttaagg tgtctactgt cagccggaat gaggttttct gggactggct
     6901 gagccgggag gctgatttaa gtgactgccg ctggctgatg cgtcgccgct ggatggatac
     6961 cgatgaggca aaagctacat tcccgggaat ggctcaggtt atcgattatg ccattgatga
     7021 ctggcgtggt tttgtcgata ccacggttac tgaaggccag cccagtccgt tgatgagtgc
     7081 atgggaagag tatcagtcat gggatcgaca gcagaacgaa tggcttcagc gtgaacgccg
     7141 tcgtgtgctg cttcaggtgg tttattaccg tacattcgag cgtcttccgg tgattgaact
     7201 cagtaatgga cgggtggtgg cctttgataa aaataatctg atgcaggcgg tagctgtggc
     7261 atccgggcgg gttcaggtga aagtcgggcg ggtaagccgt attcgtgaag cctggtttgt
     7321 cgggccgcac tttattgtgg atcgcccctg tagtgctccg caggggatgt ttccgctggt
     7381 tcctttctgg ggataccgaa aggataaaac cggggagcca tacgggctaa tttcccgcgc
     7441 cattccggca caggatgagg tgaattttcg tcgtatcaag ctgacctggt tgcttcaggc
     7501 caaacgtgtg attatggacg aggatgccac ccagttgtca gacaacgacc tgatggagca
     7561 gctcgaacgt ccggatggca ttattaaact gaatccggtc cgaaaaaatc agaaaagtgt
     7621 cgcagatgtt tttcgggttg agcaggattt tcaggttgcc agccagcagt ttcaggtcat
     7681 gcaggaatcg gaaaaactta tccaggatac catgggagtg tattccgcat ttctcgggca
     7741 ggattcaggt gcgacgtcag gcgtggctat cagtaacctg gtggagcagg gggccacaac
     7801 ccttgcggaa atcaacgata actaccagtt tgcctgccag caggtgggaa gactgttgct
     7861 ggcttatctt ctcgatgacc tgaaaaaacg ccgtaatcat gcagtggtga ttaatcgcga
     7921 tgatcgccag cgtcgccaga ccattgtcct caatgctgaa ggtgataatg gtgaactgac
     7981 caatgatatt tcaaggttaa atacacatat tgcgctggcg cctgttcagc agacaccggc
     8041 gtttaaggca cagcttgcac agagaatgtc agaggttatt caggggctgc cgcctcaggt
     8101 gcaggctgtt gtgctcgacc tgtgggttaa tcttctggat gtgccgcaga aacaggagtt
     8161 tgttgagcgt attcgtgctg cgctggggac gccaaaatca ccggatgaaa tgacgccgga
     8221 agaacaggaa gtagcggcac aacaacaggc acttcagcaa caacaggcag aactccagat
     8281 gcgcgagatg gctggcagag tggcaaaact ggaagctgac gccgccaggg cacatgcagc
     8341 tgcacaacgg gataatgcca gtgcacagcg ggaagtcgcc ctgacacagg ggcagcgtta
     8401 tgtggatgcg cttaaccagg cacatacggc agaaatcatt accggcgtac agaatatgga
     8461 acaggagcag gacgttcttc agcaacagat gctgtatacg ttacaacagc ggatgaatga
     8521 aatgtcgctc tgaaaactct ggcttcaact gaaccccgtc atcgtacggg gttttttgtt
     8581 tccggaggta agcgttccgg gagcggtgcg cttattcgcg ggggcagcga taagccttat
     8641 ttactcaacc attcggatct gtccgataaa cagaccatgc ggagttattt atggattttg
     8701 aatttacggg tgaagaaacc ccggaacaac tggaaaaaat gctggaagga cttggggatg
     8761 tggatattga cagtcacgca caggacgtcg tgacggaaga taccacggaa aaacatgcgg
     8821 atgaggaagc acagactcag acgggcgata acaatgtggc accgacgccg gatgccagtg
     8881 tggagcagac gcaggacgtg aaggagccgg aagcgaaggg ggtgctcacc cgcgacggta
     8941 aacacgtcat tccctatgaa gtccttgagg ctgaacgttc cggtaagcaa cgggccgaac
     9001 aggaagccgc acttcttcgt gggcagatag ctgaagaaaa acgcagggtg gaactgctga
     9061 cgtctcagat ccaccaggcc ggtatgaagc ccacaccgtt accggaaaac gaaaaaattt
     9121 ctgatgagca gattgcccgt atcagggaga tgtatccgga aattggtgac gcggtggctt
     9181 cgctcatccg taaaaataac tatctc
//
LOCUS       DABSYT020000144         9148 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.144, whole genome shotgun sequence.
ACCESSION   DABSYT020000144 DABSYT020000000
VERSION     DABSYT020000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.144"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..794
                     /locus_tag="ID725_RS22070"
                     /old_locus_tag="pgaptmp_004414"
     CDS             <1..794
                     /locus_tag="ID725_RS22070"
                     /old_locus_tag="pgaptmp_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGV7852875.1"
                     /translation="IKQKVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSH
                     RSVALAKTVAAPIMATGVGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDT
                     SVRNKYRPAFDKWNKLVDKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKS
                     AKDRTISIVLGNSMLQTLFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDI
                     RVLHLSNKFNVGLQGNINTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            1488..1706
                     /locus_tag="ID725_RS22075"
                     /old_locus_tag="pgaptmp_004415"
     CDS             1488..1706
                     /locus_tag="ID725_RS22075"
                     /old_locus_tag="pgaptmp_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGV7852876.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            1923..2165
                     /locus_tag="ID725_RS22080"
                     /old_locus_tag="pgaptmp_004416"
     CDS             1923..2165
                     /locus_tag="ID725_RS22080"
                     /old_locus_tag="pgaptmp_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGV7852877.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2495..3424)
                     /gene="fdhE"
                     /locus_tag="ID725_RS22085"
                     /old_locus_tag="pgaptmp_004417"
     CDS             complement(2495..3424)
                     /gene="fdhE"
                     /locus_tag="ID725_RS22085"
                     /old_locus_tag="pgaptmp_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGV7852878.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3421..4056)
                     /gene="fdoI"
                     /locus_tag="ID725_RS22090"
                     /old_locus_tag="pgaptmp_004418"
     CDS             complement(3421..4056)
                     /gene="fdoI"
                     /locus_tag="ID725_RS22090"
                     /old_locus_tag="pgaptmp_004418"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGV7852879.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4053..4955)
                     /gene="fdoH"
                     /locus_tag="ID725_RS22095"
                     /old_locus_tag="pgaptmp_004419"
     CDS             complement(4053..4955)
                     /gene="fdoH"
                     /locus_tag="ID725_RS22095"
                     /old_locus_tag="pgaptmp_004419"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGV7852880.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(4968..8018)
                     /gene="fdnG"
                     /locus_tag="ID725_RS22100"
                     /old_locus_tag="pgaptmp_004420"
     CDS             complement(4968..8018)
                     /gene="fdnG"
                     /locus_tag="ID725_RS22100"
                     /old_locus_tag="pgaptmp_004420"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7431..7433),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGV7852881.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQGNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8212..9045
                     /gene="fdhD"
                     /locus_tag="ID725_RS22105"
                     /old_locus_tag="pgaptmp_004421"
     CDS             8212..9045
                     /gene="fdhD"
                     /locus_tag="ID725_RS22105"
                     /old_locus_tag="pgaptmp_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGV7852882.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2212 a         2269 c         2382 g         2285 t
ORIGIN      
        1 caattaagca gaaagttttt ttgggcagtg aacaaccgtt gtttgtaaag cataccgcaa
       61 tgatgaggga ggcatgtaag gaattaccgc agctagtgga gtatgttcct ttagcctgtg
      121 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcaccc atcatggcga
      181 caggtgtggg cgcggcttta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      241 tttctggttc gccggatatc gagatgataa aaaaacgcat ggctgaattg tttaaccagt
      301 cggatacttc tgtgcgcaac aaatatcgcc cggcttttga taaatggaat aaactggtag
      361 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attatagcga
      421 cagcgataaa tgaaactcct gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      481 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactctttg
      541 aaaaacgcct cgccgatgat ggagaaatag aaaacctttt tgatcaacag ggatatttta
      601 gttgcgattc attaacagcc gaagaagtaa tgatgttaaa agaccttttt gatattcgag
      661 ttcttcatct ctctaacaaa tttaatgttg ggttgcaagg taatatcaac acggatgttt
      721 tacaagattc gttttttaaa aatgtcgatt tcattcatga atctaactcg tataccgtag
      781 ggatgggagt ctgaagagta aaactcatta ggattatcag aattcggcca atgcattgca
      841 aaaatgaaat acaaagagag atgtgtgcag gcgcttagcc gcgaggggca tggcgaagcc
      901 atacagaaag caccggcatt cagaactcct gtgaaagcgg catagtgccg ctgctcagga
      961 gttttgttat gacccgtatc gtgctgaacg ttgccaccct atgatgcgat taaaaaaact
     1021 caccgtcgag ccgatgattg acgtcaatgc tatcggatga tgccgggggt gttccggaag
     1081 gaattccttg tttcccgaac tgttaccaac aaaattataa cgatgagcaa gtaaagaccg
     1141 gttggtggca gtactgaccg gtctgtgatg gaagattaat ttcaccttaa atcctggtgc
     1201 tgttaggatg ctgacgtttt tcgggtacac cattcagatt atgcaaataa agattcaatg
     1261 aaaaaataaa tatccctgaa tataatatat tcgtagcacc cataactcat cctgggcgat
     1321 aactaaacca tttttgagta acaaatgaaa caactgcaac gctcttatta tttctggaaa
     1381 acgtctcgca aaaacaaaac aacatgcaaa aatccgcttt cgttaattaa tttttcggcg
     1441 taattttcgc acatacatta atgagtaata tatgtaaggt ggtggtaatg gcgatgaata
     1501 cggtttttct tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc
     1561 gtaaggtttc acgatctgca attttacgcg aagcggtaga gcaatatctg gaacgtcagc
     1621 aattcccggt gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg
     1681 atcagtgtaa ggagcataag gaatgacaaa aatttttcat tcttgaatat aaaaaacaga
     1741 tgcccttatt ctggtattaa tagaaggctg ttttacttga acttataata actgcaactg
     1801 ttacatcata tctggaaaac gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct
     1861 ttaaagattc gacaagggta cttttctctc catagatatc aaatgatatt ggtcaaatat
     1921 ttatgaactc tctggcaggt attgatatgg gcagaattat actcgattta tcgaatgagg
     1981 tgattaagca acttgatgat cttgaggtgc agcgtaatct tcctcgtacg gagctattac
     2041 gggaagcggt agatcaatac ttggtaaatc aatcgcaaac agtaagaacc agtgcttttg
     2101 gcatatggca agggtgtgag gaagatggtg tcgaatacca gcgtaagctg cgcgaggaat
     2161 ggtaatggga aaaaatgata tcaatcagat tgctgacaac gtgcgcgttg ttcatgccgg
     2221 atgcggcgtg aacgccttac ccggcctaca aagtcgtata aattcaatgt attgcagttt
     2281 attggtaggc ctaataagcg cggcgcatca ggcaatctta cgcttatcat cagtctcata
     2341 cccataacct tattgatgtg ggggtgggta aattgaggca aaacaggcac tggcggcata
     2401 gcttgaggtg gtaacgcgga atacgagaga tggtgtcgtc acaccctatg agctgtaatc
     2461 cgggcgacca gcgccacccg gaaaacgtca gagattactc cccttcaccc ggaaacagga
     2521 acgggttgat ggaactgcgg gcatagcctt cttgctccat tcgcgcgtcc agtaccagag
     2581 aggcgaggtc atctgccacg gcttcaactt tcggttcttt ttcctgatag agaattttca
     2641 ggtaagtgcc gcagtcatcg cagctttcgg ctttaatcgc ggcctgttcg tcatccagcg
     2701 accagtaatg cagtttgccg ctctgctcgc agttgctgca ttttacgcgc acaacgtgcc
     2761 attcagtttc acacaggttg cagtgcaggt aacgtagccc ctgagtggtg ccaatttgca
     2821 ccatgctgga caccggcata gaaccacaaa ccgggcaata ttgacgttgc tcgccgtatt
     2881 cagcgcgggc tttgccgggg atcagattgg ccatctgcgc ccagtagagc gacagtgcag
     2941 cccagataaa cggcgcttta tcgctgctga cggacgagaa atcagaggca aacagtgcgc
     3001 tggccatatc ttccagctcc tgagtcgatg ccttctccag attctcaatc actgccagcg
     3061 ccgggccgct catttcaggt ttcagctcag caatcagcgc catcagcagc ttttgccagt
     3121 gcttatcacg cggcagaacg tgaatatcca gcgggggctt gccttgtgcg ctggcttctt
     3181 taatgcgcgc agtcagatcc atctccagcg gatggtcgta cagcaccact tcctgggcgt
     3241 gggcgataag cgcagcaaag cgcaggtaat cacccagcgg attattttct gccagctcgc
     3301 gcagacgctc ggcgcggcgg ttgtataaat tcttgagccg agggaacaat aacggcggaa
     3361 tcatatccgc cgtacgtttc tcgctcgaac ccagctcatc ttgcgggatt atgcgaatac
     3421 tcattcagct ttcttttccg ttgtcttgcg gacctcacgg taccagcgcg ggtgatgttt
     3481 cttcgcccaa gcgctggtaa cccatccttc caccatggcg gtaatcgtgc ctttcaccca
     3541 aagggcggcg tagatatgca ccatgataac cacaattaac gccactgcgg caaatgaatg
     3601 cagcattaac gcgaatcgga tcaccgggat tgagaaagca ggcgcaaaat aaggacgcca
     3661 gataatcacg ccgctcacca gcagcagaac caggaaaata atcgccgccc agaaaacgca
     3721 tttctgaccg aagttataac gcccggtgtc acctacttcc tcgttgacga cgatcttacg
     3781 aatattcttc gcccaaaaga tatcatcccg attgattagg ttgtgatgcc agtaacggaa
     3841 aaacatgatg atgaacgagg caaacataac cacgccgaca aacgggtgca gaattcgcgc
     3901 cagctgcggt gtgcccatga tttgcatcaa ccagttgaag gacgggaaca aaaagcccag
     3961 cccgctcacc gccgccagga tgaagcagaa ggcggtgatc cagtggttga tacgttccgg
     4021 cgcggtgtag cgcacgatgg tgtcacgtcg tttcatttgc gctcctcgtc tttctcttcg
     4081 tgcagattat tctcttcctc atccgcatgg ttcggaccga cacccacgta gtggaagata
     4141 ctggctgcga aggtagccgc aaagccaaca gctgcgagcg gtttccagat gcctttccag
     4201 aatttcacgg tttcgctgat ttccgggttc tccggcaagc catgatacag atttggcttg
     4261 tcagcatggt gcagcacgta cataacgtgt gtaccaccga cgcctgccgg atcgtacagg
     4321 cccgcattgt cgtaaccgcg ggttttcagc tccgccacgc gctcgctcgc cagcgttttc
     4381 atcgactctt tcgtaccgaa gtgaatcgcg cccgttgggc aggtcttcac gcaggctggt
     4441 tcttgcccaa ccaccacgcg gtcaacgcac agcgtacatt tgtagacgcg gttgtcttcc
     4501 gggttgaggc gcggaatgtc gaacggacag cccgcaatgc aataaccgca gccaatgcac
     4561 tgctcggact ggaagtcgac gataccgttg gcatactgaa taattgcacc ttccgccggg
     4621 cacgctttca gacagcctgg atcggaacag tgcatacagc cgtctttgcg gatcagccat
     4681 tccagtttgt cgttctgctc cacttccgag aagcgcatca ccgtccacga tttggcgctt
     4741 aaatcattgg ggttgtcgta caccccaatg ttattgccga cggtatcgcg aatgtcgttc
     4801 cactctgaac acgccacctg acaggcttta cagccgatac aggtggtgac gtcgatgagt
     4861 ttcgccactt cttcctggaa gtcccgcgcc tgaggcgcgg gggtcagacc gttagtcgcg
     4921 gaacgacgaa tgatatcttg agattgataa gccatatgtc gtctccgtta caccttttcc
     4981 acattcacaa ggaaggactt aaactccggc gtctgcgtgt tcgcatcacc gacgaatggc
     5041 gtcaacgtat tggcaataaa gccttttttc gcaacacctt catagcccca gtgaatagga
     5101 ataccgatgg tatcgatatc tttgccgttc gctttcagcg tgcgaatacg tttggtcacc
     5161 accgctttgg ctttgatata gccacggttg gaggagactt tcacggtatc gccctgggca
     5221 atgccaagtt tattcgccag cgactccccg atttccacaa actgctctgg ttgcaaaatc
     5281 gcgttcaaca gcgcgtgttt ggtccagtag tggaagtgct cggtcagacg ataggtggtt
     5341 ccgacatacg ggaacttatc ggctttaccc aatgcttcgg cgtcgtcttt aaagatacgt
     5401 gcagccgggt tcgagataac gtttggatgc agcgggttag ttcccagcgg cgtttcaaac
     5461 ggctcgtagt gttccgggaa cggaccttcc gccatcttat cgagggcaaa cagacgcccc
     5521 atgccttcct gctgcatgat aaacggcccg acgccgctac ccggaggcgc tgcgctgtag
     5581 tccggaatat cccagccggt ccacttagtg ccgtcccatt tcagcaactg acgcttcgga
     5641 tcccacgggt taccctgcgg atctgcggag gcgcggttat acagaatgcg gcggttaagc
     5701 ggccatgccc atgcccaacc cagcgtgtta ccgaggccag acggatcggc gttatcacgg
     5761 cgtgccatct ggttgccttc cggcgtccag ctaccggcga aaatccagca accacaggaa
     5821 gttgtaccgt catcgcgcag ttgggcaaac gagctaagtt gttggccttt cttgacgata
     5881 accgcaccgg ttgccggatc ggtaatatcg gccagcgcct taccgttgct ctccatcgct
     5941 acttcttccg acttcggctc atgcggaatg gcgtagttcc aggtcatgtt cagcacctgg
     6001 tccgggttcg cgccaccctg ttcggcatac atcttgcgca agcgcaggaa gataccggag
     6061 aggatctcgc catcagtcag cgcaatcccc ggggcgtccg cacctttcca gtgccactgc
     6121 aaccagcgac ctgagttaac gattgaaccg ttctcttctg cgaagcaggt cgatggcaga
     6181 cggaacactt cggtctggat cttcgacgag tcaacttcgt tcagctcacc gtggttctgc
     6241 cagaagttag aggtttcagt gttcagcggg tcgatggtga cgaggaactt cagtttcgac
     6301 agacaactga tcactttgtt tttgttcggg aatgaggcaa cagggttaaa gccctggcag
     6361 atatagccat tgaccttgcc ttctttcatc atctcgaagt attgcaggac gtcgtagcct
     6421 ttatcccact tcggcaacca gtcaaagccc cagctatttt ctgccgtcgc tttatcacca
     6481 aagaaggcct tcatcataga gacgaagaat ttcgggtagt tgccccagta gtttccctgg
     6541 ccttccagca gcggttttgg cgtgttggcg gtaaggtagg tttgcagatc ggtctgcttc
     6601 tcgcttggca gcgtcatgta acctggcagg ctctgcgaca gcagccccag gtccgtcagc
     6661 ccctgaatat tggagtgacc gcgcagggcg ttaacgccgc cgccagccat ccccatgtta
     6721 ccgagcagca gctggatcat cgccatcgta cgaatgtttt gcgcaccaac ggagtgttgc
     6781 gtccagccga gggcatacag gaacgaggca gttttatcgt gagcactggt ttctgcgatg
     6841 tattcgcaga ctttcaggaa cgcgtctttt ggcgtaccac agatgttttc aaccacatct
     6901 ggcgtgtagc gggaaacgtg ctgtttcagc aagttccaca cgcagcgcgg atgttgcagc
     6961 gtggtatcgc gtttggcgaa gccgttttcg tccagttcat aagtccagga ggatttatcg
     7021 tacttgcgtt tttccgcgtc gtagccggtg aacaggccat cttcaaagcc gtaatcctca
     7081 cgcacgatca ggctggcgtt ggtataggct tcagtgtatt cgcggttgaa tttttcattg
     7141 ttcagcaggt acagcaatac gcctgacagg aaagcaatgt cagtaccgga acgaataggg
     7201 gcatagtagt cagccaccgc agccgtacgc gtaaagcgag gatcgatcac aatcagcttc
     7261 gcgccattgt gaattttggc ttccatcgcc cagcggaacc cgaccgggtg agcttcagcg
     7321 gcgttaccgc ccatcaccac gacgaggttg gcgttcttga tgtcgaccca gtggttggtc
     7381 atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacacgc
     7441 gcctggttgt cgaccgcgag catacccagc gcgcgggaga atttttgcgt taaatagccg
     7501 gtttcgttac tcgacgcgga agcacacagc atcccggtgg agagccagcg gttaacagtc
     7561 acgccttcgg cgttttgcgc aatgtagtta gcatcgcggt cttctttcat cagtttggcg
     7621 atgcgatcaa acgcctcttc ccaactgatt tgctgccatt tatcagaacc tggcgcacgg
     7681 tattccggaa acttcagacg gctttcggag tggatgaaat ccaccaggcc agcgcctttc
     7741 ggacaaagtg caccgcggtt gaccgggtga tccggatcgc cttcgatatg gaagatagat
     7801 gctttggcgt tttttgctcc gtcaccgagg ctgtacatca acagcccaca gcctacggaa
     7861 caataggtgc aggtattacg ggtttcgcgg gtgcgcagca gtttatactg ccgggtttcc
     7921 gcgagtgcta cgctgggtgc aaaacccagt gccgctgccg tggtgcctgc cataccgcca
     7981 gcgcagatct taaagaactg ccttctgctg acctgcatgg attgctcctt gtttcgacat
     8041 tgtcacgtcc catttacatt cgcttgctgc gtgtgcaggg agtgggagtt atttttcttt
     8101 gcggaagggg ccgcaaaggt ccagaattgg ctcaatttcc ctccatccag gaagggtttg
     8161 taacagaata ccataatgtt ggtgtgtgtg ttcttatctg gttaagagaa agtgaaaaaa
     8221 acacagcaaa aagaaatcga aaatgtgaca aatatcacag gtgttcgtca aattgagtta
     8281 tggcggcgtg atgatttaca acacccacgg ttagatgagg tcgcggaaga agttcccgtt
     8341 gcgctggtct acaacggcat ttcgcatgtg gtgatgatgg cgtcgcccaa agaccttgag
     8401 tactttgcgc tcggtttttc gctttccgag gggataatag aaagtccgcg cgatatcttc
     8461 ggcatggatg tcgttccttc ctgtaatggt cttgaagtac aaattgagct ttccagccgc
     8521 cgctttatgg ggttgaagga gcgccgccgg gcgctggcgg gacgtacggg ctgtggcgta
     8581 tgcggcgtgg agcaacttaa tgacatcgga aaaccggtgc agccgctacc gttcacccag
     8641 acgtttgatc tcaacaaact ggatgatgca ttacgtcatc tcaacgattt tcagccagtg
     8701 ggacaactga ccggttgtac tcacgccgcc gcctggatgt tgccatctgg cgaactggtc
     8761 ggcgggcatg aagacgtggg tcgccatgtg gcgctggaca aactgttagg ccgccggtca
     8821 caagaagggg aaagctggca gcaaggtgcg gtactggttt ccagtcgtgc cagttatgaa
     8881 atggtgcaaa agtcggcgat gtgcggcgta gagattttgt ttgcggtgtc tgccgcgacc
     8941 acgcttgctg tagaagtggc tgagcgctgt aatctgacac tggtaggttt ttgtaaaccg
     9001 ggtcgggcaa cggtttatac ccatccgcag cgtttgagca attaattaaa ataagtcctg
     9061 cgatatatat taaattgcag gacttattca tttcgtgaat tttattattt tatttataaa
     9121 caaaatgcgt gtagtggcgt tttctttt
//
LOCUS       DABSYT020000145         9109 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.145, whole genome shotgun sequence.
ACCESSION   DABSYT020000145 DABSYT020000000
VERSION     DABSYT020000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.145"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            506..739
                     /locus_tag="ID725_RS22110"
                     /old_locus_tag="pgaptmp_004422"
     CDS             506..739
                     /locus_tag="ID725_RS22110"
                     /old_locus_tag="pgaptmp_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852883.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            complement(717..1124)
                     /locus_tag="ID725_RS22115"
                     /old_locus_tag="pgaptmp_004423"
     CDS             complement(717..1124)
                     /locus_tag="ID725_RS22115"
                     /old_locus_tag="pgaptmp_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852884.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(1147..1365)
                     /gene="ydfC"
                     /locus_tag="ID725_RS22120"
                     /old_locus_tag="pgaptmp_004424"
     CDS             complement(1147..1365)
                     /gene="ydfC"
                     /locus_tag="ID725_RS22120"
                     /old_locus_tag="pgaptmp_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGV7852885.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(1438..1737)
                     /locus_tag="ID725_RS22125"
                     /old_locus_tag="pgaptmp_004425"
     CDS             complement(1438..1737)
                     /locus_tag="ID725_RS22125"
                     /old_locus_tag="pgaptmp_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852886.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            complement(2001..2408)
                     /locus_tag="ID725_RS22130"
                     /old_locus_tag="pgaptmp_004426"
     CDS             complement(2001..2408)
                     /locus_tag="ID725_RS22130"
                     /old_locus_tag="pgaptmp_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7852887.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            2485..2712
                     /locus_tag="ID725_RS22135"
                     /old_locus_tag="pgaptmp_004427"
     CDS             2485..2712
                     /locus_tag="ID725_RS22135"
                     /old_locus_tag="pgaptmp_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGV7852888.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            2696..3247
                     /locus_tag="ID725_RS22140"
                     /old_locus_tag="pgaptmp_004428"
     CDS             2696..3247
                     /locus_tag="ID725_RS22140"
                     /old_locus_tag="pgaptmp_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGV7852889.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            3219..4259
                     /locus_tag="ID725_RS22145"
                     /old_locus_tag="pgaptmp_004429"
     CDS             3219..4259
                     /locus_tag="ID725_RS22145"
                     /old_locus_tag="pgaptmp_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGV7852890.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            4252..4713
                     /locus_tag="ID725_RS22150"
                     /old_locus_tag="pgaptmp_004430"
     CDS             4252..4713
                     /locus_tag="ID725_RS22150"
                     /old_locus_tag="pgaptmp_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGV7852891.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            4801..5481
                     /locus_tag="ID725_RS22155"
                     /old_locus_tag="pgaptmp_004431"
     CDS             4801..5481
                     /locus_tag="ID725_RS22155"
                     /old_locus_tag="pgaptmp_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGV7852892.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            5478..5642
                     /locus_tag="ID725_RS22160"
                     /old_locus_tag="pgaptmp_004432"
     CDS             5478..5642
                     /locus_tag="ID725_RS22160"
                     /old_locus_tag="pgaptmp_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852893.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            5647..6180
                     /locus_tag="ID725_RS22165"
                     /old_locus_tag="pgaptmp_004433"
                     /pseudo
     CDS             5647..6180
                     /locus_tag="ID725_RS22165"
                     /old_locus_tag="pgaptmp_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            6341..7099
                     /gene="paa"
                     /locus_tag="ID725_RS22170"
                     /old_locus_tag="pgaptmp_004434"
     CDS             6341..7099
                     /gene="paa"
                     /locus_tag="ID725_RS22170"
                     /old_locus_tag="pgaptmp_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HGV7852894.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            7435..7590
                     /gene="hokD"
                     /locus_tag="ID725_RS22175"
                     /old_locus_tag="pgaptmp_004435"
     CDS             7435..7590
                     /gene="hokD"
                     /locus_tag="ID725_RS22175"
                     /old_locus_tag="pgaptmp_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGV7852895.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            7811..8068
                     /locus_tag="ID725_RS22180"
                     /old_locus_tag="pgaptmp_004436"
     CDS             7811..8068
                     /locus_tag="ID725_RS22180"
                     /old_locus_tag="pgaptmp_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852896.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            8138..8416
                     /locus_tag="ID725_RS22185"
                     /old_locus_tag="pgaptmp_004437"
     CDS             8138..8416
                     /locus_tag="ID725_RS22185"
                     /old_locus_tag="pgaptmp_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852897.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            8418..>9109
                     /locus_tag="ID725_RS22190"
                     /old_locus_tag="pgaptmp_004438"
     CDS             8418..>9109
                     /locus_tag="ID725_RS22190"
                     /old_locus_tag="pgaptmp_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGV7852898.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT         2457 a         2040 c         2360 g         2252 t
ORIGIN      
        1 acgttaacca gttaacaatt aacgccggat acagagaatc cacccataac actgttttta
       61 gctttaactg ttccgcgtgc gcttagccgc attcactgca tcacaaaatt cactttaaaa
      121 agggcggaca tcagttcatg ggcaaacaga tgccgccaaa cgtcaccaga aaattgataa
      181 cagagggcgt tgcagcgggg ttgtcactta agcgtatggt caacctgaca acccggtgtc
      241 ctcaacgggg aaggaataac cccgccatac ttaccgctgc gccatttcgc ggagtgccac
      301 aaccggaagc gcacggtcga cgaaaattta acgacaggct atctatgaac cagcaacctc
      361 gccgtgcgct ttcgcgttat gctctgactt ttcagagaaa tatcctttca gtaaactgtc
      421 agtgccggat gttcacccgt gtccggcgca cgcactccac ttcacccgtg gagaactcct
      481 taattaccaa ccctcaggag ggtgaatgtt aaaatcaact cttattgcta aatgccttta
      541 tcaaaatcgc atggtaagca gcatttcaat aggcgagtct gcagttaaaa gtattttcga
      601 agagtacttt cccgggcatg attttaataa atggaatacc aaattaccgc cagcagtttc
      661 aacgcgtatt ctgaaagcaa ccgaaagagc aagtacaatt cgcgttaact atttcattaa
      721 agatttgtgg gatctttgat atccacagag cctaaagtat gtgcatatgg atgtgctatt
      781 atgcgccctc gcagatttgc atcattttct aaattcactg aacgaaacag ggcatcaaca
      841 aggctctgta caatgcaaag gcaatcgaag actgtcgccg tttctgtttt gattgatgaa
      901 agaacatggc cattcacgca aacagaaatt acccgtttat taacatcgct ttcctgcttt
      961 tgattatcag aaccatatag cccagaaaaa gcattgcgca cattacgaac catattatcg
     1021 atggtttctt tttctgcggt actaaggtca agagtagcca gttgtgaacg aactatattc
     1081 gatgccattt cctgtaatgg cgttggtaaa tctttaaatt ccattattag cctcgttggt
     1141 tagctattaa cgtgggtatg taatcattct ggcaatgctt aatgccgctg ctttttccag
     1201 attggtgata tcctgctcca gagcggacag attttcagcc tgcttagccc tggcttcatt
     1261 ggcccatttc aggtcctgca ccgccttaat tttctggtgc atccactcat aaagttcatc
     1321 atcggtatag tctggcgcga tgatgacggg gtctcgtttc tgcatgtcgg ctccttgtgg
     1381 ttagcgttgc ctgcttttta accacgtcag gcgaggtggt atcctctgag gggtctgtta
     1441 ctcgagagga aattggttat gaatacaatc aagttttctt gcccagaatg tggtggctaa
     1501 gtctttgaca catcctttaa gccgcagggc tctgacagtt tcgcgggagc catctgcaaa
     1561 aattgtggtc accttgtaac tgaagatgag tcctcgcagt tcgatgacga aatcgttgac
     1621 aatatcttcg gtgcactcac cagagacttt ctgaagtaaa ggcgcatacc gcttagttac
     1681 cgctctgata actcttacct gtccggcaat ggcgctgata tcaatataaa gcgccatcgc
     1741 tgtttctttg ctgatccctg gacgccttcc attctgatgt ttgactcgcc cactgagaaa
     1801 tcctctgctt ccccttaacg ccgggtagcg gaactgtttg ctgagaacac cgtgcggtgt
     1861 cttgatgagt agaatttaga atagcctaag agttatggtc aagctttttg tgtagaaaaa
     1921 cctaagcttc ttgatgtaaa aaacacaagt atttgaaagt ttgtgctttt tattacagag
     1981 agttgcgaaa aaaagggggg ttatttattt gcgcttcttt tgcgagcttt gagtagttct
     2041 tcaaaaagtt tgttgaaatt ctcaactcga gcacgcatct ctgacaacag agccttttgc
     2101 tctgactcag gcagtgcgtc gaacagttga agcaactctt tttgatcttc tgtcagattg
     2161 actggctgat tatctgggat cggttcgcct ggttgcttat cttcatctcc aaaaagaagc
     2221 caagtcggcg agcactgaag cgcctggctc agtgcgaata atctcttccc cgctggctgt
     2281 gtttcatctc tttcccattg agaaattgtt acgtgagcca ctttgaccag cttacctaat
     2341 gcggcctgag acagttttaa tttttttcgc ctgtataaga ggcgagcacc gaaggtttcg
     2401 tttttcatat taggtaattc taatttttct tgacttaggt ttctctacaa tctagtttcc
     2461 ttaggaaaat ctaagggttt cgatatgttg aaaattgatg ctatagcgtt ttttggcagc
     2521 aaaacaaagc ttgccaatgt cgcaggagtt aggctggcaa gcgttgctgc atggggggaa
     2581 ctggttcctg aaggtcgcgc gatgcgcctg caagaggcat ccggcgggga acttcagtac
     2641 gaccccaaag tttatgacga atatcgtaag gcaaagcggg cggggcggtt gaacaatgaa
     2701 aatcaccact gaacaggttt gtgaggctct ggatacctgg gtatgccgac caggaatgac
     2761 acaggagcag gcgacgatat taatcacgga agcattctgg gctctgaaag aacgcccgaa
     2821 catcgatgtt caacgcgtca cgtttaatga tggcgaggtt gatcaacggg cgctgggcgt
     2881 taaccgggtg aagatattcg aacgctggaa agctatcgac accagagata agcgtgacaa
     2941 attcacggcg ctgattccgg caattatgga ggctatccgg atcagcgatt tcagattgta
     3001 ttgtgaaatt actgacggaa aaagcattac gtacatgatc gccgggttaa acaaagaata
     3061 tggcgatgtg gtggagtccg ggctgctttt tgcggatcca gttgttgtgg aacgtgagac
     3121 tgacgagctt atagaaaaag ctattgcttt caagcacgcg tatcgtcagc aataccaata
     3181 ttactttgca gataaacaaa tgtctgccag gggggcgtat gagtatcgat gcactacgat
     3241 gggctaaaaa ggtgaaaacc ggcagttcat ccagtaagtc agtattgacc tggcttgctg
     3301 atatgtgcgg tgccgatttg tgtgcatacc cgtctgtatc tgcactggca gaagtaacgg
     3361 aactgaacaa aaagactgtg caggacagct tacgacacct gatggagatt gggttaattg
     3421 ttgataccgg tgagagaaaa ggcagaacaa agcaaattgt ggtgtaccga cttatcggtg
     3481 tagaagaaag tgttgccgag cctgaataca cccaaaaacg ggagtcttta aaggtgggta
     3541 aaattggtgc tgttaataaa aacagtaccg aaaatggtta tgtttcagca caaaacagac
     3601 ccaaaaacag aactcttagc tgcatggaaa ataaccaaag acacccaaat tttccatcaa
     3661 agacacccaa aaacggatca cggaacccaa aggaacccaa agatctaaac cccacacata
     3721 acgcacgcga gagtgctccg accagtgagc aggaagtttt gtcgttacag gcagcccccc
     3781 ctgtattcct gtatggcctg agcgaaccca tcggaaaatt cccgatgagc gatagctggt
     3841 atccgtcacg ggattttcga cgacgggctg cgttgtgggg gatggctttg ccggagacag
     3901 aatttacacc tgctgaactt gccgccttcc gggactactg ggcagcggag gggaaagtgt
     3961 ttacgcagat tcagtgggag cagaaattcg cccgtcacgt aaatcacgtc agggcgcagg
     4021 ttaaaccagt cagcaaaggg gtaaaccatg cagcagcacc aggtggcacc gcatcacggg
     4081 cagttcagga aattcgggca gcacgtgagc agtgggaacg tgaaaacgga tttatcagcg
     4141 acggaaacgg cctggaagct gtgggaactc atgggggtgg tttattcgaa ccgctggatc
     4201 cagaagaacg gggccgcacc ttcgaagctc tggattgcac agattggcgc gatgactgag
     4261 cagcaaatcc ggcaggtctg ccgccagtgc atggaccgct gccgggcggg tgaaacatgg
     4321 cctccggacc tggctgagtt tgtggcactg atttcgaaaa gcggagccaa tccattcggt
     4381 ctgacggtgg atgctgtgat ggaggagtac cgccgctggc gcaatgagtc ctggcgatac
     4441 gacggaagtg ataagtaccc gtggtctcag cctgtgctgt atcacatttg cctcgagatg
     4501 cgttcaaagg ggattgagcg ccagatgacc gaaggggaat taaaacggct tgcagaacgg
     4561 caactgacga aatgggcaaa gcatgttagt aacggcctga gcgttccgcc agtccggcga
     4621 caactggcgg cccccaaacg cccgtcgggg ccaacgccaa ttgagttgct gaaacaggaa
     4681 tatgaacgcc ggaaagcggc tggttttgtt tgagttgaga agtgattttt taccgggagg
     4741 aaatttatgg agactgtttt tgacgcactg aaagcgatgg gaaaagccac gtcggtatag
     4801 ctggctgcgc gacttgatat cagtcgtgaa gaagtactga acgagctgtg ggaactgaaa
     4861 aaggctggct tcgttgataa aagcgtatac acctggcgtg tggctgataa caacgttcag
     4921 caggaacagc cagcgccaga agaaaccacc acggcaacag aagcgaaaat ctcagagtgc
     4981 gatttaaccg cgacgattga acaacgcgga ccacaaacgg cggatgaact ggctacgctg
     5041 ttcggtacaa catcccgcaa agttgcttca acgctggcaa tggcaatcag caaaggtcgt
     5101 ctgattcgcg taaaccagaa cggtaaaatt cgttactgca taccaggtga taatttacca
     5161 gcagagccga aagttgaatc ggtagcggaa accgatggta aagcctttcc tcagccagcc
     5221 ggtgttgcgt taccggtaca gaaagatgca acacaggaag atattaaaac agaaactgtg
     5281 gcggacattg tgcagtcgct gccatcgttt actgcaacgc gagaagatga tttgattttg
     5341 ccatcgctgc atatggcaaa tcgcgaactg cgtagggcga agaatcatgt ccagaagtgg
     5401 gagcgagtct gcgccgcgct gcgggagctg aacaagcacc gggatatggt tgccgggatt
     5461 tgtcggaagt ccgggcaatg agcggatggt gcaggcctga aatcatgata ctaacaatga
     5521 aggtaaaatg catcggcagt ctgattggtc gtagtgaggc ggcggtcagg atgaaagccc
     5581 aggttaaggg aataagcctg attctgcggg gtgattttca ccagtcaaca aaatatccgt
     5641 agcgcgataa cggtcaaaaa ttatggcgct gacacttttg tgccactgga gatgactgta
     5701 cctaagttca ggggagaaga acacgtccgg tgggatggtc gggccagatt taaagggcag
     5761 gtcatggctc cagcctgtac gctggcaatg gaggctgcct ggcgggaaat tgatatggga
     5821 accacgccac tcagggattt actgccggtc cagagaataa attcctgtta cggttacacc
     5881 actgtgatct tgcaagtgca ggaaagtagg tctacacggt aacgcgagtg cgtgtaactt
     5941 ttgatgtcat tcccgtagaa acaccggaca aattttcgct gacaggtcat gcagaaggta
     6001 taaatctgca gattatggac aattacggat atccggcaag agccggaaaa agcatgccgc
     6061 ctctaattct cagtggaaga tggacttgat tatactcatt gcattgtcag aaatagttat
     6121 ccattaaagg ctggctattc caaacaggat gttgattaca aaaatgtaat caacatgtaa
     6181 ggtttatact cttcaatatg cgtataattt tccttatttt gttgacttta aataacaagc
     6241 tatgcacgag gtaaagtcgg ataagtttat ctggatgtaa tatatattat ttgtagtgtt
     6301 tataacttta tttcatgata accaataaaa ggagtttttt atgaggaaca taatggcagg
     6361 ttttttaata ttcctgtctt ctgctgctta tgctgatatc aatctgtatg gtcctggtgg
     6421 cccgcataca gccttgcttg atgcagccaa actttatgcc gaaaaaacag gtattatagt
     6481 gaacgttcat tacggcccac agaacaaatg gaatgaagat gccaaaaaaa atgcagatat
     6541 cttgtttggc gcatcagaac aatctgctct ggctatcatt cgggaccata aagacagctt
     6601 cagtgaaaaa gatattcagc ctctttatct gcgaaaaagt attttactgg taaagaaagg
     6661 taatcctaaa aatatccgga gtattgacga cctgaccaga cctgggattg gcgtaattgt
     6721 taatgatggt ggtggtacca gtaatacatc aggcactggc gtctgggaag atattgccgg
     6781 acgtaaaggg aatatagaaa ctgtcgccgc aatccgaaaa aatattattt tatatgcgcc
     6841 caatagcgga actgcacgta aggctcttga gaatcagcct ggagcagatg tctggataac
     6901 ctgggctgac tgggcagcca gtaatccaga aattggtgat gtcgtggaaa tagcgccaga
     6961 ctacgtgata tggcgtgata tgaacattac agtacgtcag gatgcaaatg atgaaacccg
     7021 tcgatttgca gaatggctac aaaccgatga agcggcgcct gcattcaaaa aatatggctg
     7081 gaccaggaaa ggcacttgac atcctcgtcc ttcaggacgt ggattctttt tccggatgcc
     7141 gcgccagcgg catgtagggg cagctcacaa aacggaaaaa attgtacgct aagcctcgcc
     7201 aggtgaactg aattcattcc gatatgggaa ttcccatatc gggcgaaaac ggtttgctgt
     7261 aacggcagag ttaagtagga ttgctgcggg tgcttgaggc tatctgcctc gggcatgaac
     7321 accaacggca gatagataaa agccccaccc gactataaat cgaagtgagg cccctatatg
     7381 ctcgtcacat atagattgcc tcttacggac cgaaaggtca aggagaagca ggctatgaag
     7441 cagcaaaagg cgatgttaat cgccctgatc gtcatctgtt taatcgtcat agtgacggca
     7501 ctggtaacga ggaaagacct ctgcgaggta cgaatccgaa ccggccagac ggaggtcgct
     7561 gtcttcacag cttacgaacc tgaggagtaa gagaccaggc gggggagaaa tccctcgcca
     7621 cctctgatgt gtcaggcatc ctcaacgcac ccgcacttaa cccgcttcgg cgggtttttg
     7681 tttttatttt caacgcgttt gaagttccgg acggcgccgg aatagaatca aaaatactta
     7741 agtagcgcgc agggagaaga gggatggacc ccgaacaggg gagtgctatt tatctggaag
     7801 gattctgttg atgagaatcg aagaattacg tgaaattttt agtgaagatg gcctctatac
     7861 tgtgcgcgtt gagaagggcg ctattgtcag ccactgccgt attaaatgtt tacagtctca
     7921 acaaaggaag agtggagctg cgttaattca ttttgtggat gggcttgtga cggatggttt
     7981 tattttgcgt gcaaatgaat ttgtcacatc gttgccgtct ctgaaagaag ctgggattaa
     8041 ggctggtttt tctgcttttg aagattagtg aattcatcta caattcagcg cagggctgaa
     8101 cccctgttga gtaacactgt gccaccggag aaagccgatg gcgcaaaatt ccagactaca
     8161 caattctgat aattcagccg tctttgccag caggcacggg cggcgttctc atgcattcaa
     8221 atctgactgg ttccggcacg ccccatgcac tgaagaacag gccgaatggc tgattcagaa
     8281 ctaccgcaga cgtgggtatg agtttaggaa agccctcagc ctcgattatc gtcactggat
     8341 aatctacgtc aggctgccgt actccgagcg cccaccgcgt ccgtcccgca cattccagca
     8401 acgcatctgg aggtaacgtg cgggtattac ttcgacctgt tccggtaccg gaacttgggg
     8461 tggtggtcct taaaccaggc cgtgaatcca tgcaggtatt ccataaccct cgagtgcttg
     8521 tggagccgga gccgaaaagc atgcgcggtc tgccgtccgg catcgtccct gccgttcgcc
     8581 agccgctggc ggaggataaa tcattactgc cgtttttcag caatgagcgt gtgattcgtg
     8641 ctgttggtgg cgctggtgca ctgtctgact ggctgttgcg tcatgtcaaa tcctgccagt
     8701 ggcctcatgg tgactatcat cacagtgaaa ccgttattca ccgttatggt accggcgcaa
     8761 tggtgttgtg ctggcactgc gacaaccagc tgcgtgacca gacatccgaa tcactcgagc
     8821 aacttgctca tcaaaacctg tcagcatgga tgattgacgt catcggtcac gcaataagcg
     8881 gtacgcagga gcgtgaatta tctctggctg aattatcctg gtgggcggtc cgcaatcagg
     8941 tggcggacgc gctaccggaa gcggtattac gtcgttcgct ggggttgcgt gcggaaaaaa
     9001 tccgctcaat gtaccgtgaa agcgacatcg taccgggaga gcagaccgcc accatcatac
     9061 tgaagcagcg cacaaaaaat cttgcgccgc tgcctcacgc ccaccagca
//
LOCUS       DABSYT020000146         9073 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.146, whole genome shotgun sequence.
ACCESSION   DABSYT020000146 DABSYT020000000
VERSION     DABSYT020000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.146"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            180..608
                     /gene="uspC"
                     /locus_tag="ID725_RS22195"
                     /old_locus_tag="pgaptmp_004439"
     CDS             180..608
                     /gene="uspC"
                     /locus_tag="ID725_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGV7852899.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(615..2039)
                     /gene="otsA"
                     /locus_tag="ID725_RS22200"
                     /old_locus_tag="pgaptmp_004440"
     CDS             complement(615..2039)
                     /gene="otsA"
                     /locus_tag="ID725_RS22200"
                     /old_locus_tag="pgaptmp_004440"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGV7852900.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLV"
     gene            complement(2014..2814)
                     /gene="otsB"
                     /locus_tag="ID725_RS22205"
                     /old_locus_tag="pgaptmp_004441"
     CDS             complement(2014..2814)
                     /gene="otsB"
                     /locus_tag="ID725_RS22205"
                     /old_locus_tag="pgaptmp_004441"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGV7852901.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2981..3967)
                     /gene="araH"
                     /locus_tag="ID725_RS22210"
                     /old_locus_tag="pgaptmp_004442"
     CDS             complement(2981..3967)
                     /gene="araH"
                     /locus_tag="ID725_RS22210"
                     /old_locus_tag="pgaptmp_004442"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGV7852902.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3982..5496)
                     /gene="araG"
                     /locus_tag="ID725_RS22215"
                     /old_locus_tag="pgaptmp_004443"
     CDS             complement(3982..5496)
                     /gene="araG"
                     /locus_tag="ID725_RS22215"
                     /old_locus_tag="pgaptmp_004443"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGV7852903.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELYLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5566..6555)
                     /gene="araF"
                     /locus_tag="ID725_RS22220"
                     /old_locus_tag="pgaptmp_004444"
     CDS             complement(5566..6555)
                     /gene="araF"
                     /locus_tag="ID725_RS22220"
                     /old_locus_tag="pgaptmp_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGV7852904.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7352..7855
                     /gene="ftnB"
                     /locus_tag="ID725_RS22225"
                     /old_locus_tag="pgaptmp_004445"
     CDS             7352..7855
                     /gene="ftnB"
                     /locus_tag="ID725_RS22225"
                     /old_locus_tag="pgaptmp_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGV7852905.1"
                     /translation="MATAGMLLKLNSQLNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7935..8186)
                     /gene="yecJ"
                     /locus_tag="ID725_RS22230"
                     /old_locus_tag="pgaptmp_004446"
     CDS             complement(7935..8186)
                     /gene="yecJ"
                     /locus_tag="ID725_RS22230"
                     /old_locus_tag="pgaptmp_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGV7852906.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8301..8387)
                     /gene="azuC"
                     /locus_tag="ID725_RS22235"
                     /old_locus_tag="pgaptmp_004447"
     CDS             complement(8301..8387)
                     /gene="azuC"
                     /locus_tag="ID725_RS22235"
                     /old_locus_tag="pgaptmp_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGV7852907.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8649..8972
                     /gene="yecR"
                     /locus_tag="ID725_RS22240"
                     /old_locus_tag="pgaptmp_004448"
     CDS             8649..8972
                     /gene="yecR"
                     /locus_tag="ID725_RS22240"
                     /old_locus_tag="pgaptmp_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGV7852908.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         2355 a         2285 c         2056 g         2377 t
ORIGIN      
        1 gtacattatt ttcttataat tacagttgaa atcatatgat caccgagagt gattgattaa
       61 ttgtctttac taataatcgg actaatattt acctgtttga ctgagttggg attgcgtcgt
      121 ttctccatta ggagtaaagc tttaatgtca cctgaagttc acagaataag gaacaggtta
      181 tgagctatag caatattctt gtcgctgttg cggttacacc ggaaagtcag caactgctgg
      241 caaaagcggt atctatcgcc aggccagtaa agggacacat cagtttaatt actctcgctt
      301 ccgacccgga aatgtacaat caattagctg cgccgatgct tgaagattta cgcagcgtga
      361 tgcaggaaga aacgcaaagc tttcttgata agttaattca ggatgcgggg tatcccgttg
      421 acaagacgtt tattgcctac ggtgagttaa gcgaacatat tctggaagtt tgtcgcaaat
      481 atcatttcga tttggtgatt tgcggtaatc acaatcacag tttcttttcg cgagcatcct
      541 gctcggcgaa aagagtgatt gcctcaagtg aggtcgatgt gctgttagtt ccacttacgg
      601 gagattaacc gctcctacac aagcttagga aaggtagcaa ctttatcgcg ctgctggctt
      661 tccgcgcttc gcggaactat ctgctttagg tcgctaatga agcactcctg ccagtggtta
      721 atatcgtttt tcacgataac gtccagcatt tctgcatgac gggaaatacg ttccgccagc
      781 gacatagtca atgcacgatc cagcgcagct gcaacttcgt cacgatcgta gggattaaca
      841 attaacgccg acgttaactc gtttgccgct cccgcaaatt gcgaaagaac aagaacgccc
      901 gggttggctg ggtcctgagc ggcaacatac tcttttgcta ccaggttcat cccgtcacgc
      961 agtggcgtca ctaagcccac gtcagagtag cggaatattt tcatcagtaa tttacggtca
     1021 aaatgctgat tcaaataata aagcggcgtc cagcctaatt gcccgtattt accattaatt
     1081 cgtccggctt cattttcgag ctgatgacga atatcctgat aggcttgcac atcaccacgc
     1141 gacgttggtg caatctgggt ataacgaatt ttaccatgat gctgcggata tttttccagc
     1201 aacgcttcat aggcgagaaa acgctctggc aaacctttgg aataatccag ccgttcgaca
     1261 gaaaagatat tttgtacgtt tttcagttcc gctttaagtt gcgccagttt tggcggcagt
     1321 ggcccggcag cctgtttggc tatttctttt ggctcaatgc caatcgggta aacttctgtt
     1381 cgaaacgctt tgccccaggc tgtatggctt tttgcgctac gtgtcgtgac gcgggtcagg
     1441 ttagaaaggc aatccaggaa cgccagacga tcgttttctg tctggaaacc cagcaaatca
     1501 taatcacaaa gctgttcaag caaggtgtca tatgtcggca gcgcgttgaa gatttccggt
     1561 gtcgggaaag gaatatgcag aaagaaacca atgcgattat tcactccccg tttgcgtaat
     1621 tcatgcgcaa atggcaacag gtgataatcg tggatccaga taatgtcatc gtcttgcaac
     1681 agcggcagta atttatctgc cagcaacgca tttacgcgta gatagccgtc ccaggcagga
     1741 cgctgaaatt gcaccagatc gaggcgataa tgaaaagcag gccagagaac ggcattggag
     1801 aattggttgt agtattcgtc aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg
     1861 ttaccctttt tcaccttttt tagcggctga tcctcattcc ctgtttcacc actccagcca
     1921 aaccacagtc cgcctgcggc tttcagtgcc cccagtatgc caacggcaag gccaccggca
     1981 ctggcggcgt gctcatctgg tggtgcaatc cggttagata ctacgactaa acgactcata
     2041 gtcatcactc ctgttatttt ctcttttttg ttgtaatgcg gtggttatca tttcaagcca
     2101 gctccagaca tccggtacgc ccgccagtcg ccatgatgcc tgagttgcgc ctgtgccaat
     2161 ttttactgac attccgccca gtcggttaac gactgcgaag ccagattcat cggttaaatc
     2221 atcgcccaga aatacgggcg ttcgcccgat aaagggagct tcctgcataa aggcagcaat
     2281 cgcctcacct ttactggtac ctctcggttt gatctcgaca acacactttc cctgctgtaa
     2341 cgccatttgt ggccagatct gagtaatacg ttgcgctaat gtcattaatg cgtcttcatg
     2401 ctgcggagcc tgacgataat gcagcgcaaa agccatccct ttcgtctcca gctccgcacc
     2461 gggatactgg gcgatgaccg tatgcagttg cacgttaata tcacgtgcaa tcgcatccgg
     2521 cagatgaacg atatgcgttt taccattgat gtcacggcgc tccgccccat gcacgcccgc
     2581 taacgggaag cgataaggtt ttgccagtgc gtccagctcc accattgagc gccctgatat
     2641 caatgccaat gcaccatcac ttgcggttgc cagtagctga agtccttgca gaatattgtc
     2701 aggtacgacg acctgatcgg gatgcggttt gatttccgcc agcgttccat cgagatcaaa
     2761 aaaccaggca tatttcgcgg atagttcagg ggtttcggtt aacggttctg tcacccggtt
     2821 ctcctccttc tcttttctta ttgtgttgcg cagaacgcgc tttacagaca tgtaagtata
     2881 gacagtgtga cgggggtcgc catttgcaac gaagaacagc caaccgttgt ggcggctggc
     2941 tgtggtggga aaaaacgtta aattgttgtg gaaaaaagca tcagacagtg cgtttcgctt
     3001 tttgcttgta acggtcgaag atcaccgctg ccagcaggat taagccgcga accacgtact
     3061 gcgcgaaagg agaaatatta agcaggttca tggcgttttc cacggtgcct aaaattaaga
     3121 tacccgccac cacatatgag atttttccga tgccaccttt cagagaaacg ccacctaaaa
     3181 cgcaggcgga gataacaata agctcataac caatcgacgt cattggctgc ccactggtca
     3241 tacgtgaagc cagaataatt ccggctatcg cagataccag gccagagaga acaaagataa
     3301 taattttggt gcgagcaacc ggtacacccg ccagacgcgc agcctcttcg ttcccgccaa
     3361 tcgccagggt attacgacca aaggtagttt tattcagcag caaaccaaag ataatcagac
     3421 acgcgacggt gagccagatt ggcgcaggca gaccgaacca gttggcgtaa ccaagggcaa
     3481 agaagctttc atcttcgata ccgaccgctt taccgtcgga aatgatgtac gccagacctc
     3541 gaacaatctg catcgttgcc agtgtcgtga tcagagcatt tattttcagt ttggcgataa
     3601 caaagccatt gaccaggcca cagagaatgc caagcagcaa ccccgctgcc acgccaatcc
     3661 acaggctttc agtcagattg ataaccaccg ccgtggtgac acccgcacag gcaattacgg
     3721 aggcgacaga aaggtcaaag tcaccggaag caaggcagaa caacatgcca cacgccacca
     3781 tccccgacat ggaaattgcc aggcccaacc ctttcatatt aataaaggtg gcaaagttcg
     3841 ggacaaaaat ggcacaggcg ataaaaagca ccgcaaacac caccagcatg ccgtactgat
     3901 cccagatacg cccgaagctg aatgacgact taggtgcgcc agaacccgat gtagaaacag
     3961 aagacatcat actctcctta ctcaggcaac tgcctggctg actttaggca tcgcaaggct
     4021 cagtgcctga cgctcatctg cctgctcgtg taacaattca ccggcgattt caccttcgcg
     4081 catcaccaca atccggtcgg caacgccgag gacttcaggt aagtcgctgg aggcaaacag
     4141 caccgccaca ccctgcgccg ccagcgcata aatcacgtta tatatttcgt gcttagcacc
     4201 aacatcaatg ccgcgcgtag gttcatccag caaaatgacc ttcatctctt ccgataacca
     4261 gcggcccaga atggcttttt gctgatttcc acctgagaga ttcataatca gttgctcagc
     4321 gcccggcgtt ttgatgttga gcgaacgaat gtggtgatcg gcattgtttt cttcccaacc
     4381 gttgttgatt acacaaccgc cgagcacatg tttacgtctg gcactgatgt tgatattgtc
     4441 gcgaacggag tgcacaggaa taatgccttc cgctttgcga tcttccgggc agagcatcat
     4501 gcctgcggca atggcgtggc tcggtttacg aatatcgatc ggctgttggt cgatataaac
     4561 ctgaccggcg gtgatttgcg tcccgccaaa catgcctttc attaattcgc tacgccccgc
     4621 ccctaccaga ccaaacagcc caacaatttc accactgcga accgccagac ttattggcgt
     4681 acgcacgcct ggtgctttca cagcatcaag acgcaggcgc tcctcgccat aactacgcgg
     4741 ttgccagccg tagatatcgc caatgtcgcg cccgaccatc gcctgcacca gcgcgtcgtg
     4801 gtcaacctgc tgcatatcgg taaaggtttt gacataacgt ccatctttaa agacagtgat
     4861 ggcatcgctg agggcaaata tttcttccat acggtgagaa acgtataaga tcacccgccc
     4921 ctcttttcgc agttcacgaa taacgcggaa aagattgtcg atttcgcggg cagaaaggga
     4981 gctggttggc tcatcaaagg cgataatttt agcgttacgc gccagcgcct tggcgatttc
     5041 aaccatctgc cactgaccaa tggagagata tttcagcggc gtgtccgggt caatatccat
     5101 accaagatgt ttaagttgta aacccgcctc ataattcagc aatgagcgat tcacaatgcc
     5161 gcctttatgc ggcagctggc cgagatagat gttttccgcg acggtcattt ccggcacgag
     5221 atacagttcc tggtaaataa tcgccacacc cgcgttaagt gctgcggtcg tgtcggaaaa
     5281 ggacatttcc tgcccattaa tcactacaga acccgtggtt ggcgcatagt tgccgctgag
     5341 gatttttaag agagttgatt ttcctgcgcc attttcaccc atcaacgcat gaacctgacc
     5401 ggcatagcag tcaaaactaa tatccgtcag cgccttaacg ccgggaaacg ttttaccgat
     5461 gccgcgaaat gagagatacg gggtagactg ttgcataacg tctccgtgaa tcactggtcg
     5521 ttcacacccc ctctgcatca tgcaaagggg aatttttccg gcaaattact taccgcctaa
     5581 accttttttc tccagttctt ctttaaagtt gtcacgcgtg atcagcacca cgtcggtaac
     5641 ttcggtgaat ttggtcggtt caacaccttt tgctacccag ttgtaaagca tttcgctgga
     5701 cttataacca tgtacgtccg ggcttggcaa cagggaaccg tagaagccgg ttgcctgtgc
     5761 cttagacagt tcgctcaccg catccacacc gttaataccg ataccgatga tatcggccgc
     5821 tttaaagccc tgaccttccg tcgcgcgtac gccgcccagc acggtgctgt cgttcatacc
     5881 gacgatcagc caatgtttaa cttccggatg ttgaaccagc attgagttgg cagcgtcaaa
     5941 tgccccgggg atgtcgttag atttggtagg tacctgataa atttgttttt ccgggaatcc
     6001 tgcagctttc agcgcatcca tagaacccgt agtacggcga cgagccgtat ccagttcgtt
     6061 ggaggtaatc gccatcaccg cgctttcttt gacatcccag ccacgtttct gcatctcttt
     6121 atacagttcc tggccctgac gttcgccaat tttggtcgcc gccatcatca ccagaggaac
     6181 ggtatccatt ggcttacctt tggcgttaac aaactggtca tccacggcaa tgactttcat
     6241 atcgtagcca cgcgctttcg ccacgatggc agagccgagt ttcgggtccg gagtacaaat
     6301 aacgaaacct tttgcgccac tggcggccag gctatcgatc gcgttcaatg ttttttcgcc
     6361 atccggcacg gcaatcttaa taacctcaaa ccctaaatcc ttcccggctt tatcggcaaa
     6421 cttccattcg gtctggaacc acggctcttc cggttgcttc accaggaaac cgagcttcag
     6481 gttctccgcc atagcggatt gtgacataac ggctgccaga ccaatggctg ccagggcttt
     6541 agtaaatttg tgcatggttc tctccagctt tagtgtcgtt ttgtgcaggg caaaaacgaa
     6601 tgacattcgt taaattaatc ggaaaacaaa gcattacctt ttaactaaaa gataagtgac
     6661 tgtgttgaca tagttttagc gagaaattaa ttctccatag gagagcaata tcacatcgca
     6721 gaattacagt gagaacgtgc ataaatttag cgggaaaaga cataaggaaa agccaatttg
     6781 tcagacaaat tgttgaatgc acagcagatt aatccataag attagcctgg aaatccttgt
     6841 tgtctttggt acccatgcgg gatgtcttct ttttcaccag tcaataggcc gcattacctg
     6901 gcgttgagtt tttgaaatgg tgtaataacc gcaactcaaa gatgtggaaa atgcacgtca
     6961 ttcatttcgt cattaattat cactgtgctc attaattaac agaacacgta taatgagagc
     7021 catctcgcaa aaatgaaaaa acgttttata aaataatcac ttcatcatga attcaaattc
     7081 attgattaat atcaacaaga tacaaaaagc actatcatta aaattcattg cagttacatt
     7141 gatttcatca atgaaatgta aaaatatata aacttgatga tttaagcatt ttcttatacc
     7201 cgttcagacg ttattcttat ttcagatcat cgtcagaatt gactccacga tcacatttcg
     7261 gaccggcaga aaggaattat tctgcaaaca gtaattatgg tgttttgatt tatcttgcac
     7321 ctctccactt ctggatataa ggatattagg tatggcaacc gctggaatgc ttctcaaact
     7381 caactctcaa ttgaaccgcg agttttacgc atccaatctc taccttcacc tgagtaactg
     7441 gtgttctgaa cagagtctga acggcaccgc cactttcctt cgcgcccagg cacagagtaa
     7501 tgtgacccaa atgatgcgca tgtttaactt tatgaagagt gtcggcgcta cccccatcgt
     7561 taaagccatt gatgttcccg gtgaaaaact gaactctctg gaagaactgt tccaaaaaac
     7621 gatggaagaa tacgagcaac gttctagtac gttggcacag ttagccgatg aagcgaaaga
     7681 actgaatgat gattcaaccg tcaatttcct gcgcgatctg gaaaaagaac agcagcatga
     7741 tggcctgttg ctgcaaacca ttcttgatga agtgcgcagt gcgaaacttg cgggtatgtg
     7801 cccagtgcag accgaccaac atgttctgaa tgtcgtgtcg catcagctgc attgatcata
     7861 catcgacgct aatgcattgc accgatgaag tttttgagaa accgctgcct catctttttt
     7921 gaagcagcgg ttttttaatg ggattcgcct tgtggggtaa acttgagctc aatcagcgcg
     7981 atggcttttt ggattgcccg catggtgacc gggtctgcgg cagcgggatg atgagtaaag
     8041 tcgatattct tcagctgact ggacattttt tcacgaactt caacgggcgc gattacatcg
     8101 agaacatcca gaatttgttt gataaccaac tggcaagcaa ccacatcaga aaccagttcc
     8161 tgatcggcat tcagcgtctg ggacatcgta aactcctgat agcattttga aagccgttat
     8221 agtagcggct tcacatcttc aacgatagtc acatccaccc tcatcaggac acaaaaaaac
     8281 ctgccggagc aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc
     8341 ttgcaatagt tattgaacat acttttcagg attttgcgca gtttcatcgc ggcactccga
     8401 ccatttgtta tacaggtgtt attgtctttg cgcttataat atgacaacca tcacaaaaat
     8461 caatctttat gtgatacaaa tcacataaat acccctttaa tgttataaaa atgataatca
     8521 aagaacagcc ccctatttct gacacctaca gatggcaaga aatagcgcct gccaggcgtc
     8581 ttttccggcc attgtcgcag cgctgtaacg cgtaaaatag tgctttctct tactcttctg
     8641 gctggaccat gagacttctg attctgactc tttcattaat aacgctggcg gggtgtacgg
     8701 ttactcgcca ggcccatgtg agcgaagttg acgcggcaac aggtattgta cggttggttt
     8761 atgaccaggc ttttttgcag catgcccata ccgatcgtta tgtcagtcgc gggatctccg
     8821 atcgcgcatg ccaacaggaa ggctataccc acgcgatccc ctttggtcag ccagtaggca
     8881 actgtagcct ttttgcgggt tccttatgtt tgaatacgga attcactttg tcatatcagt
     8941 gccatcattc cgccttccct gtctttctct aaatatttta atcagcgagg ggatcttcgc
     9001 tgattaaaga aatagcggta atgcgtttta ttcccattcg tatttttaat aattgaagtt
     9061 tatattttac ctt
//
LOCUS       DABSYT020000147         8879 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.147, whole genome shotgun sequence.
ACCESSION   DABSYT020000147 DABSYT020000000
VERSION     DABSYT020000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.147"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            2..2149
                     /gene="espL4"
                     /locus_tag="ID725_RS22245"
                     /old_locus_tag="pgaptmp_004449"
     CDS             2..2149
                     /gene="espL4"
                     /locus_tag="ID725_RS22245"
                     /old_locus_tag="pgaptmp_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HGV7852909.1"
                     /translation="MNNTAQTNEHQTLSELFYKELEDKFSGKELATPLLKSFSENCRH
                     NGRHIFSNKDFVIKFSISVLQADKKEITIINKNENTTLTQTIAPIFEEYLMEILPQRS
                     DALDKKELNLNSDRKEKEFPRVKLNGQCYFPGRPQNRIVCRHIAAQYINDIYQNVDYK
                     PHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKMESND
                     LSVMAAILLVDNHALTVRLRIKNTTEGCIHYVVSVYDPNVTNDKIRIMSESKEDIKHY
                     SLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGTVDEITPPLSPATMNLL
                     MAIGQNHQLKQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAINYGNADIVETIFNSL
                     SEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSILNALPKLAATHHLD
                     NEQVYKFLSAKNSTSSHVLYHVMANGDADMLKIVLDALSLLIRTCHLTKEQVLDLLKA
                     KDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASDIVDLLTAKSLARDTGLF
                     MAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNV
                     VEMVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELACELGHRVIAELIFNTLNK
                     MAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(2118..3854)
                     /gene="aceK"
                     /locus_tag="ID725_RS22250"
                     /old_locus_tag="pgaptmp_004450"
     CDS             complement(2118..3854)
                     /gene="aceK"
                     /locus_tag="ID725_RS22250"
                     /old_locus_tag="pgaptmp_004450"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGV7852910.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(4038..5342)
                     /gene="aceA"
                     /locus_tag="ID725_RS22255"
                     /old_locus_tag="pgaptmp_004451"
     CDS             complement(4038..5342)
                     /gene="aceA"
                     /locus_tag="ID725_RS22255"
                     /old_locus_tag="pgaptmp_004451"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGV7852911.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5372..6973)
                     /gene="aceB"
                     /locus_tag="ID725_RS22260"
                     /old_locus_tag="pgaptmp_004452"
     CDS             complement(5372..6973)
                     /gene="aceB"
                     /locus_tag="ID725_RS22260"
                     /old_locus_tag="pgaptmp_004452"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGV7852912.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(7242..8171)
                     /gene="metA"
                     /locus_tag="ID725_RS22265"
                     /old_locus_tag="pgaptmp_004453"
     CDS             complement(7242..8171)
                     /gene="metA"
                     /locus_tag="ID725_RS22265"
                     /old_locus_tag="pgaptmp_004453"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGV7852913.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8328..8771
                     /locus_tag="ID725_RS22270"
                     /old_locus_tag="pgaptmp_004454"
     CDS             8328..8771
                     /locus_tag="ID725_RS22270"
                     /old_locus_tag="pgaptmp_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGV7852914.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
BASE COUNT         2314 a         2154 c         2152 g         2259 t
ORIGIN      
        1 tattaataat acagcccaga caaatgaaca ccaaaccctg agtgaattgt tttataaaga
       61 actcgaggat aaattttctg gcaaggagct ggcgacacct ctattaaaaa gcttctcaga
      121 gaactgtaga cacaatggtc gccatatctt tagtaacaag gattttgtca ttaagttttc
      181 catatctgtc ttacaagctg ataagaaaga aattacgata attaataaaa atgaaaacac
      241 gacactcact caaaccattg ctccaatatt tgaagaatac ctaatggaaa ttttacctca
      301 acgctcagac gctcttgata aaaaagaatt aaatctaaac tcagatagaa aagaaaaaga
      361 attcccaaga gttaagctta atggtcaatg ttattttccg gggcgccccc aaaaccgtat
      421 agtatgccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgactacaa
      481 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
      541 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
      601 cggtgatttc gggctagtta tgaaggcata ttttgacaag atggaatcaa atgacctcag
      661 tgttatggct gccatattat tggtagataa ccatgctttg acggtccggc taagaataaa
      721 gaacacaact gaaggatgta tccattacgt ggtttcggtt tatgatccta atgtaactaa
      781 cgataaaata agaatcatga gcgaaagtaa agaggatatt aaacactatt cactgatgga
      841 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
      901 tgttgcaata attccagcac ttccaaaaga acagctattg atgctaaaag gaactgtgga
      961 tgaaataacc cctccattat cacctgcaac gatgaatttg ctaatggcaa ttggtcagaa
     1021 tcaccaactt aagcaactga tgattcaact ccagaaaatg ccagaactac atagaacaga
     1081 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
     1141 taatgcggat atcgttgaga ctatttttaa ttcattgtca gagccaggat atgaaggatt
     1201 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
     1261 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgcctt
     1321 acctaaactg gccgcaacac atcatttaga taacgaacaa gtgtataaat tcctgagtgc
     1381 caaaaatagt acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
     1441 gctgaaaatt gttttggacg cgttatcttt gttaattcgc acatgtcatt tgactaaaga
     1501 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaggac tatacctggc
     1561 gatgcaaaat ggacatagcg atatcgtgaa agttattctt gaagcattgc ccagcctggc
     1621 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
     1681 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
     1741 ttttaacgca ttacccactc tgtttaatac gtttaaattc gataaaaaaa atatgaagcc
     1801 cctcctcctg gcaaataatt ctaatgaata cccaggtttg ttttcagcga tacagcataa
     1861 acagcaaaac gttgtagaga tggtttatct tgctttatct gaccatgcac gcctgtttgg
     1921 atttaccgct gaagatatta tggatttttg gcaacacaaa gcgccacaaa aatactctgc
     1981 ctttgagttg gcttgtgaat tgggtcaccg ggttattgct gaattaatct ttaatacatt
     2041 aaataagatg gctgaaagct ttggctttac ggataaccct cgatacattg cggagaaaaa
     2101 ttatatggaa gctttactca aaaaagcatc tccccatacc gtacgctaaa tctttgcctg
     2161 cgccgatacg cataaacatc ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc
     2221 cagtaatcag cgcggaacag gtcggcgtgc atctcttcaa acagcggacc aatacgcggg
     2281 tcggcgcata gccaatggcg aaactcttcc gggaaaacat cgcccggcga gacgctgtac
     2341 cacggttcgc tggcaagttc gtcttccggg tagcgcggcg gcgggatatc gcggaaattc
     2401 acttccgtca tgtagcaaat ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg
     2461 acaccgaagt ttttaaacag catgtcgccc gggaaaatgt tagcagcggc aagctggcga
     2521 atggcattgc cgtactcttc aatggcgtcg cgcagctgct gaccttccac ttgttccagc
     2581 cagatattga gcggcaccat ccgccgctca atataaagat ggcgaatcac aatttgttcg
     2641 ccgagatcgg tgattttttc cgctgcttcc tggagcagta attccattaa tgccggggaa
     2701 atatgccgct tctccagcac aaagttttca aactcctggg tatccgccat tcggcccacg
     2761 cgatcgtgct ctttcaccag ttgatagcag gcacgaacgt gagcggcaga catctctttc
     2821 tgcggcgcga acttgtcttt gatgacttta aatacacggt caaagcccgg cagcgtgaac
     2881 accaacatca ccatgccacg aatacccggc gcttcaatga actgctcatt acagccctgt
     2941 agataaacga gatattcgcg gtagctttcg gttttggcgt gcttctggca gccgatagcc
     3001 atatacaatt cagcggtggt tttacctggc agaatttccc gtaaccactc gaccagcgct
     3061 gcgggcagcg gcgcataaac cataaaataa gaacgcgcaa agccaaaaac aatgctcgct
     3121 tcggcggtcg tcgtcagaca ggtatcaata aacagttcgc cgtcatccgt ctggtggatc
     3181 ggcaataaaa atggcaatgt gccggaaggc gttattagtt tgcccaccag ccaggcggct
     3241 ttattacggt aaaacagttc gttcgccacc tgtaaatgac ttttggagag gttttctggc
     3301 cccaacgttt ccgtcagatg gcgaataatg taatggatgt cacggctttt attctgccag
     3361 tgcagacgca acggcaggtc gctgataacg cgcatcagca gagattccca gccgtgatcg
     3421 gggtgaaagt ctttcgccag cgggcgggga atggtacgaa agcggcgctc tggctgggag
     3481 ctaaagataa aaagccgctc gggagtaagc gagcggtggt caaataaccg acagtacacg
     3541 gagttaaaaa agctctccgc aatctcgaag cgcgggtaat ccggcaacag ccgggtgtaa
     3601 tgctctttga cgcgcagtaa aaattccgcg tccgtgcttt ggccgttggt aatgcagcgc
     3661 agttgctcca cgaccagacc aacgtgatga tcgtaaagat ggatacggtt tttcatcgcc
     3721 tgctggacag catgccagtc ggcttgttcg aaacgctgct gcgccccgga agtcacttcg
     3781 aggaatcgac catactgagc gtcgaagcct tgcaaaatgg tttgagcaat cagtaattcc
     3841 aggccacgcg gcatttagcg ccctcatcag gagcagagaa ttgccggatg cggcgtaaac
     3901 gccttatcca gcctacgttc ggcaacggtt gtaggcatga taagacgcgc aagcgtcgca
     3961 tcaggcatcg tgcaccgatt gccggatgcg gcgtatatgc cttatccggc ctacagtcag
     4021 caacgtttgt aggttgctta gaactgcgat tcttcggtgg agccagtcag cgcggtaacc
     4081 gacgacgtgc cgccctgaat aatggtcgtc actttatcga agtaacctgt acccacttcc
     4141 tgctggtgag atacgaaggt gtagccatct ttcgcggcgg cgaattccgg ctgctgcact
     4201 ttctcaacat agtgcttcat accctcgccc tgggcatagg cgtttgccag atcaaacatg
     4261 ttgaaccaca tgctgtggat acctgccagg gtgatgaact ggaacttgta gcccatatcc
     4321 gacagctgct gctggaagct ggcaatagtg ttgtcgtcga ggtttttctg ccagttgaac
     4381 gacggcgagc agttataagc cagcagtttg cccggatatt tcgcgtggat agcttccgca
     4441 aagcgacgcg ccagttccag atccggcgtg gaggtttcac accataccag gtcagcatat
     4501 ggcgcatacg ccaggccacg gctgatcgct tgctcaatgc ccgcatgagt acggaagaag
     4561 ccttcactgg tacgctcgcc ggtaataaat tcgctgtcat acgggtcgca atcggaggtg
     4621 atcagatccg ccgcatcagc atcggtacgt gcaaccagca gggttggaac gcccgtcacg
     4681 tcagctgcca gacgcgccgc gaccagtttc tgaatagctt cctgagttgg caccaaaact
     4741 ttgccgccca tgtgaccgca tttcttcact gacgccagct gatcttcgaa gtgaactgcc
     4801 gctgcaccgg cttcaatcat cgctttcatc agttcaaagg cattcaggac accgccaaaa
     4861 ccggcttccg cgtcggcaac gatcggcagg aagtaatcga catagcgcgg atcgcccggc
     4921 tcaatgcccg cggaccattg gatctgatcc gcacggcgga aagtgttgtt gatccgctcc
     4981 accacagctg gcaccgagtt tgccggatag agcgactgat ccggatacat gctggccgcc
     5041 aggttagcgt ccgccgctac ctgccatccc gacagataga ccgcttcaat acccgctttc
     5101 gcctgttgca gcgcctgacc gccagtcagt gcaccgaggc tgttgatgta gccttttttc
     5161 gactcgccat gcagcagacg ccacattttc gccgcgccca gttgcgccag cgtgcattca
     5221 ggattgactg aaccgcgtaa tttcaccaca tcttccgcgc tgtatgggcg agtaattcct
     5281 tcccaacgcg gttgagtcca ctctttctgt aattcttcaa tttgttgtgt acgggttttc
     5341 atgtgcagat gctccatagt tatgtggtgg tttacgctaa caggcggtag cctggcaggg
     5401 tcaggaaatc aattaactca tcggaagtgg tgatctgttc catcaagcgt gcggcatcgt
     5461 caaaacgccc ctgggagaaa cgttcttcgc ccagttcgct ggcaatgact ttcatctctt
     5521 cgcccagcat ctggcggaac aaggctttag ttaccggttt gccattgctc aacgtttttt
     5581 gatgatggat ccactgccag attgaggtac gggaaatttc agccgtcgcc gcatcttcca
     5641 tcaggccata aatcggcacg cagccgttgc cggagatcca tgcttcgatg tactgcacag
     5701 ccacgcgaat gttggcgcgc ataccttctt cggtgcgttc accatcacaa ggtgccagca
     5761 gctgatcggc agtaatcggc gcgtcttgtt cgcgcatcac ttcaagctga tttttacggg
     5821 agccgagaat gtcgttgaat accgccattg ccgtgtccgc aaggcctggg tgagcgatcc
     5881 atgtgccatc gtgaccgtta ttggcttcca gcgatttatc cgcttttact ttgttgagca
     5941 cctggttatt gtgctcttca tctttgctcg gaataaacgc cgccatgccg cccatcgcaa
     6001 aagcaccgcg tttatggcag gttttaatca acaggcgtga gtaagcattc aggaatggtt
     6061 tatccatcgt tactgcctgt ctgtctggca ggacgcgatc gggatagttt ttcaacgttt
     6121 tgatatagct gaagatgtaa tcccaacgac cgcagttcag accaacaata tggtcacgca
     6181 gcgcgtgaag gatttcatcc atctggaaca cggcgggcag cgtttcaatc agcaacgtcg
     6241 ccttgatggt gccgcgcggc agattaaagc gatcttctgc atagctgaag acttcgctcc
     6301 accaggccgc ttcctgccag gactgggttt tcggcagata gaaataggga ccactgccct
     6361 ttgccaacag tgcctgatag ttgtggaaga aatagagcgc aaaatcaaac aggctgccgg
     6421 ggattgcctc accacgccag gtgacatgtt tttccggcaa gtgcagaccg cgtacccgac
     6481 aaatcaaaac cgctggattg ggcttgagct ggtaaatttt gcctgcttca ttggtgtaac
     6541 tgatggtgcc gttaaccgca tcacgcaggt taatttgccc gtcgatcact ttgttccagt
     6601 ctggtgccag tgaatcttcg aaatcggcca taaagacttt cacattggcg ttgagcgcgt
     6661 tgatcaccat cttgcgctct acggatccgg taatctctac gcggcgatct tctaagtccg
     6721 caggaatccc gcgaattttc cagtcagcat cacgaatgga agctgtttcc gaaataaaat
     6781 caggcaacgt tccgttatca atatcttgct gctgctgaat gcgcgctgcc agaagtttat
     6841 tgcgttgtgg cgtaaaatgc gtcaccagct cagtcagaaa ttctaccgct tcggcagtaa
     6901 gaatttgctt ctcctgctcg ccatacggcc ttatgaaagc cagttcatcg gttgttgttg
     6961 cctgttcagt catcgtgcag ctcctcgtca tggatccgaa aacttcccca ctgtgaacga
     7021 aggatcgttg tgcgcttttc gttcagcaca actaagacta ctcatttaaa aatcaaaatc
     7081 aaaaacaatt tccattttaa ttaaaaatac ttgataactt attgataaca atatgaataa
     7141 aatttattca cctactgagg tgtgtttcgg aaataaaaaa ggcacccgaa ggtgcctgag
     7201 gtaaggtgct gaatcgctta acgatcgact atcacagaag attaatccag cgttggattc
     7261 atgtgccgta gatcgtatgg cgtgatctgg tagacgtaat agttgagcca gttggtaaac
     7321 agcaaattac cgtgactacg ccagctcgct cgcggtttgt tttgcggatc attgtgcggg
     7381 aaatagttat acggtacttc cgggtctagt ccggcttcca catcgcggaa atattcctgc
     7441 gccagcgttt gcgcatcata ttcgggatgg cccgtcacaa aggcaatgcg cttatcttta
     7501 ctggcaaaca gatatgcatc cccttcttcc gtctctgcca gaatttccag atcggtgtaa
     7561 tcacgaatca acgctgccgg aaagtcagca tagcgcgaat gcggtgccaa gaatgaatca
     7621 tcaaagccac gcgtcagaag cgcatgagga tggagaatat gatgctcgta aacgccagag
     7681 agtttgtcgg tgcgagtttg cttaggaatg ccgtagagga tattgagcgc tgcctgtacc
     7741 gcccagcaga caaacagcgt cgaggtgacg tgatctttcg accactccag cacctgtttg
     7801 atctgcggcc agtaagcgac atcattaaac tccaccaggc ccagcggcgc accggttaca
     7861 atcagaccgt caaagttctg ttcctgaata tcttcaaagt tacagtagaa gttgttcaga
     7921 tgctctgcgg gcgtgttacg cgattcacgg gaatcgatgc gcaacagctg aatatcgacc
     7981 tgcaaaggtg agtttgaaag caggcgcaga aactgatttt cagtttcaat cttcttcggc
     8041 atcaggttaa ggatcagaac cttaagtgga cgaatttcct gaccagacgc acgagaagtt
     8101 gtcatcacaa agacgttttc ttcacgcaag aaattgacgg cgggtagctc gtccggcaca
     8161 cgaatcggca taacctgatt acctcactac atacgcttat acgtttagac atccagatag
     8221 ctgaagataa ccagaaaatt tgccaatgtc gagcctgcat gttgaaggtg agaaagtttc
     8281 aaggaaaaga ctacgttaga atataagaat gactaaaagg agagaaaatg gttattagta
     8341 ttcgccgctc acggcatgag gaaggggagg aactcgttgc gatttggtgt cgatctgtcg
     8401 atgccaccca cgatttttta tcagcagagt atcgggccga gctggaagag ctggttcgtt
     8461 cctttctgcc ggaagcaccg ttgtgggtcg cggttaatga acgggatcag ccggttgggt
     8521 ttatgttgtt aagtgggcag catatggatg cgctgtttat cgatcctgat gtgcgcggct
     8581 gcggcgtggg ccggatgctg gtgaagcatg cgctctcaat ggccccggaa ctgacaacca
     8641 atgttaatga gcaaaatgag caggcggttg ggttctataa gaaggtgggt tttaaggtta
     8701 cgggacgctc tgaggtggac gatttgggga aaccgtatcc gttgctggat ctggcgtatg
     8761 tgggggaata agctgccccc tatcgtgcct gcgtaagctg gataaaacgc gccagcgtcg
     8821 catccggcat ttttttgcac caacgcaaaa aggccatccg tcaggatggc ctttcgctt
//
LOCUS       DABSYT020000148         8836 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.148, whole genome shotgun sequence.
ACCESSION   DABSYT020000148 DABSYT020000000
VERSION     DABSYT020000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.148"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(293..397)
                     /locus_tag="ID725_RS22275"
                     /old_locus_tag="pgaptmp_004455"
     CDS             complement(293..397)
                     /locus_tag="ID725_RS22275"
                     /old_locus_tag="pgaptmp_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852915.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(513..1097)
                     /locus_tag="ID725_RS22280"
                     /old_locus_tag="pgaptmp_004456"
     CDS             complement(513..1097)
                     /locus_tag="ID725_RS22280"
                     /old_locus_tag="pgaptmp_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGV7852916.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLDDWISCSERMPDDGQHVIILCDGAFVLYAQYRDGE
                     FFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            complement(1154..1549)
                     /locus_tag="ID725_RS22285"
                     /old_locus_tag="pgaptmp_004457"
     CDS             complement(1154..1549)
                     /locus_tag="ID725_RS22285"
                     /old_locus_tag="pgaptmp_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGV7852917.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(1565..2334)
                     /locus_tag="ID725_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /pseudo
     CDS             complement(1565..2334)
                     /locus_tag="ID725_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(2360..3100)
                     /locus_tag="ID725_RS22295"
                     /old_locus_tag="pgaptmp_004459"
     CDS             complement(2360..3100)
                     /locus_tag="ID725_RS22295"
                     /old_locus_tag="pgaptmp_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGV7852918.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            complement(3107..4069)
                     /locus_tag="ID725_RS22300"
                     /old_locus_tag="pgaptmp_004460"
     CDS             complement(3107..4069)
                     /locus_tag="ID725_RS22300"
                     /old_locus_tag="pgaptmp_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7852919.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(4092..4517)
                     /locus_tag="ID725_RS22305"
                     /old_locus_tag="pgaptmp_004461"
     CDS             complement(4092..4517)
                     /locus_tag="ID725_RS22305"
                     /old_locus_tag="pgaptmp_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGV7852920.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(4514..4816)
                     /locus_tag="ID725_RS22310"
                     /old_locus_tag="pgaptmp_004462"
     CDS             complement(4514..4816)
                     /locus_tag="ID725_RS22310"
                     /old_locus_tag="pgaptmp_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGV7852921.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            4914..5285
                     /locus_tag="ID725_RS22315"
                     /old_locus_tag="pgaptmp_004463"
     CDS             4914..5285
                     /locus_tag="ID725_RS22315"
                     /old_locus_tag="pgaptmp_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852922.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            5306..5497
                     /locus_tag="ID725_RS22320"
                     /old_locus_tag="pgaptmp_004464"
     CDS             5306..5497
                     /locus_tag="ID725_RS22320"
                     /old_locus_tag="pgaptmp_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HGV7852923.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            5499..5777
                     /locus_tag="ID725_RS22325"
                     /old_locus_tag="pgaptmp_004465"
     CDS             5499..5777
                     /locus_tag="ID725_RS22325"
                     /old_locus_tag="pgaptmp_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGV7852924.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            6130..6429
                     /locus_tag="ID725_RS22330"
                     /old_locus_tag="pgaptmp_004466"
     CDS             6130..6429
                     /locus_tag="ID725_RS22330"
                     /old_locus_tag="pgaptmp_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852925.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDFATTFWARKLDCIHNQFPLE"
     gene            6501..6719
                     /gene="ydfC"
                     /locus_tag="ID725_RS22335"
                     /old_locus_tag="pgaptmp_004467"
     CDS             6501..6719
                     /gene="ydfC"
                     /locus_tag="ID725_RS22335"
                     /old_locus_tag="pgaptmp_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGV7852926.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            7288..7476
                     /gene="dicB"
                     /locus_tag="ID725_RS22340"
                     /old_locus_tag="pgaptmp_004468"
     CDS             7288..7476
                     /gene="dicB"
                     /locus_tag="ID725_RS22340"
                     /old_locus_tag="pgaptmp_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGV7852927.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            7473..7664
                     /gene="ydfD"
                     /locus_tag="ID725_RS22345"
                     /old_locus_tag="pgaptmp_004469"
     CDS             7473..7664
                     /gene="ydfD"
                     /locus_tag="ID725_RS22345"
                     /old_locus_tag="pgaptmp_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGV7852928.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            7757..>8836
                     /locus_tag="ID725_RS22350"
                     /old_locus_tag="pgaptmp_004470"
     CDS             7757..>8836
                     /locus_tag="ID725_RS22350"
                     /old_locus_tag="pgaptmp_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGV7852929.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETESDPM
                     NVTFDNLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKEAGKYTSVVDLARA
                     RANQQHTENSTGKINPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNGREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPE"
BASE COUNT         2272 a         2327 c         2015 g         2222 t
ORIGIN      
        1 atacagatga cgatcagggc gattaacatc gccttttgct gcttcatagc ctgcttctcc
       61 ttgcctttcg gcgcgtaaga ggctaaccta catgtgtcta gcatgaaatt ggcctcagat
      121 taatgttagg cgtcttgcag gacgcgtaat gttaactggg gcttttctct gtctgcctta
      181 cggtggcatg cccgaggcag acagcctcaa gcacccgcag caattctact taactctcgc
      241 tttaccgcaa accgttttta cccgatatgg gaattcccat atcgtaatga attcagttcc
      301 ctagtcgatc catcaaaaac acaaccaggc agtaaacgcc cacaacagca ataacagcca
      361 gcgcaccttc cattgccagt gaaatatcat ccgacatatt ccctcctttg gtgtgaatcc
      421 cggcgaacgt ttttaccccc accgacaaat aacatatact aaaaaatcaa tagctatagc
      481 aacgcctgta attgcaaagg cttcaggcca gatcattggc gcacctcctg cggcggttct
      541 ggtagcggca tccagtcggt tacattgcgg ctctgtgttt cgaaaaattc atcaccatta
      601 cggactacat caaaaaactc accgtctcga tattgcgcat aaagaacgaa tgcgccatca
      661 cataaaataa ttacgtgctg accatcatcc ggcattcgct cactacagct tatccaatca
      721 tccagagtta ccggatagtt ggttgacgtt tccgagattt cccgaaaatt attggttgac
      781 gaaccctttt tttcccgaaa gtttccagcc tgaagcatgg cggcgctgtc tggcggggca
      841 gcatatagcg gcacgtatat ttccggttcc ttatcagcac cgggttgctc ttccagtgag
      901 aatgtctttc cggtaaatcg attcatataa agcacgggct ctgcttccag cgaagcctga
      961 gcaacaaggg ccagtgctaa atccaactca attgcctcga gagaattttt gaatgctgtc
     1021 tgttttactg caaatttcat cgcctttaca ttttcactaa catgactgat taactgctct
     1081 tttgtaaaag tggtcatctc attctccttt gatgcgaatg ccagcgacaa ttgaagcctg
     1141 atagctaatt cactcacagt accgcctcct gaaaattgcc ctgatagaac gccagtacac
     1201 gcagcataac ttcactcttc cggcactcgc cacagattat gttctgttgt ctgtcgtagc
     1261 ggcgtatttc tccgtctggt aactttcgaa tcaatgtctg gtcggttgct ttctccgctg
     1321 ccttacgcca tacgcgatac acctgttctg atgtaaaaac accgtattta ccgggcatgt
     1381 ataaatcgcc acaagccagt acatccacaa ggcaacgtct gactgaatgc cagcctgctc
     1441 ccgtcgctct ctccagttgt gatatcgtca tgcgttcatt tttgcgtacc agcccgataa
     1501 ttcgggcctt cagttcttca cgctgttcgt gtgtaaaagg tttcgccata agcgcctccg
     1561 gctatcactt ttccgataca acacggcggg aagaatcagt aatctgtcga acaatatccc
     1621 ggtgcttgtt cagctcccgc agcgcggcgc agactcgctc ccacttctga acatcacttt
     1681 tcgccctgcg cagcgccagg tttgccctgc gcagggacgg aaaaatcagc tcatctgctt
     1741 gcgtttcggt aaacgatggc aacggctgca caatgtccgc cacagtttct gttttaattt
     1801 cttcctgtgt tgcggcttcc cggactggta acgcagcacc tgctggctga ggaaaggcct
     1861 taccatcatt tttcgttacc ggcgcggctt tcggatctgc tggtaaatta ccccccgaca
     1921 tgcagtaacg aaatttaccg ttctgattaa cgcgtgccag ccgccccgtt gcggttacca
     1981 ccgccagcgt ggaagcaacc ttgcgagtac tgacgccgaa cttacccgcc agttcctcac
     2041 acgttttagc accatcctga ccgataaact caaccatcat gtctgcggta acttttggag
     2101 cgacctcttc ggttaccaca tccggcgctt caggttgtag tgcctgccct tcggttaccc
     2161 cggcttcacc ttcgacagcc agaaaccagg tgtgacccgt tttatcaaca acgccatttt
     2221 tttgagttcc cacagttcgt taagaacttc ttcacggctg atatcaagcc gcgccgccag
     2281 ttcaacagaa ttggcttttc ccatcgcttt cagtgcatgc aatacagttt ccatcgaaaa
     2341 tttacctcgt caaaaattct cacataccct gacgtccaac gtttgaccgc cagctctccc
     2401 agttaaaatt cacccaacga ccaccattca tggtcatacg gtccatcacc cgatctccga
     2461 ggagtgtgct catcgctgcg tgattcaggt tcgtcagcat tccgacacta cgcatcgaag
     2521 ccgttctgcg gtcgactatc tggttcagcg tgacctgctc gttgcgcgta tcccgctgca
     2581 tgccaatttc atcaaggacc agaaggtcaa ctccacaaag ctcctgtaaa aatttttccc
     2641 cggactggcc gttgtcgtag ccgtcatgca acacgctcat gacatcggac acggtgacga
     2701 taatcacgct tctccccttc gccatcagcc gattgccaat cgctgctgcc aggtgatttt
     2761 taccggtacc aggtttaccg ctgaacacga agtttgtaca tccggtcatc aattcatcgg
     2821 caatggattt cgcctggctc agagcatggc gctgaccgtc gttctgcacc cggtagttcc
     2881 cgaatgagca cttcctgtga agcggctgga tgcccgcacg gttcaggatt ttttcaaccc
     2941 gcacctgatg attcaggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     3001 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgc
     3061 gaatacgttc aagaacccca actgccgcaa tgtttttcat gacacgtcac cccctgaatc
     3121 ccggcggtat ttcagtgtcc ggttcagaaa tgtgattcac gcaacgctgc gcgggcgaac
     3181 gccccaggcg gataaccagt tcatcccatt tttcccggag ttttgccgga ctcatgatgt
     3241 tttttaccca gaacgaatcc cgctggagac gcccaaacat ttcacaaatt tgtctgtgag
     3301 ttctgccatc cagcatccgc attgtccgca cgtcattggc ccatgcagtc cagttgggtt
     3361 ctttcggtct cgtgatctcg ccatcatcgc tggccgcctg ctcgtaaaga ctcacgattc
     3421 gtccccagat ccactgtgcg cacaccaaat cttcctgact tccccactgg cgttttttcg
     3481 cactgaacac aaccgcgtca gggtgtcggg ttaaaaaatt ctgttcagcc gtctgcgggt
     3541 ccggttgcga agcgtccgga caagaagatc ttttatctga cggatcaggt tttaatactg
     3601 acggatcggg gtcaatcatc gcccccctaa tcggcagttt gttatcaaca gttgatccat
     3661 caaaatttga cgggtcaacc gttgaggggg caatatttga cgggtcaact gttaacgggt
     3721 cattttttgc cgggctaatt tttcttttcg gtttatatga ctcacgcgcc gccgcagcag
     3781 ctgcttcgag tttttccaca ttaagccgat agatattgct tacattacgc ccaccgacct
     3841 tacgctcttc cttcgtcagc cagccctctt tcgccagttc tgcaatagcc gatttcactg
     3901 tggattcact tcttgcaccg atctgacgcc ggatagtttc aatggcaggc catgacacgc
     3961 cctcgtcatt gctgtagtct gcaagacggg ccataaccgc caccctggat aagatcatgc
     4021 cggtgaaggc gcacccttcc cagacaagac catgaagctt gctgctcata aaacccccga
     4081 acaccgtgct tttagtgcat caccacagca ttccctgccg ggccgccgcg attcatctgg
     4141 tcatacaaaa caaccgctga cgcaacaaaa tcatcgacat ccttcaccag ccgatccctc
     4201 cgttcgacga tctcacggta atattcagaa ctgtggctgc gcatacgggc caccagcaaa
     4261 ggcggcatcg ccttttcgat cgccggtaac agagcctgca ttttttcaac agcatcaggg
     4321 gtgtctttat ccagccaacg gaaaattttc tgggtattac gggccagggc ttccggatgg
     4381 ctgtcgtcat acagttccgg gaacgtcatt cccagctcga aatacgcttt ggtaattttc
     4441 gcagccggta ctttttcgcc gtccggatgc gcccagacat tcatcgccat gcggatgtgt
     4501 tcatgcttga ttttcatgaa tcaactccat cagataagca tgcactacaa tcaccttcag
     4561 catgaactac atgtgtttgc cccaaacgaa tgccgctcgc atactcaggc caaataagct
     4621 cccaatcatg gggtcgtagc tccgccctac ttacttggcc ttccgtcgca gattcgatca
     4681 taagggcgcg ggttggagat atagctgttc gtccagacgc catttgcgat aagtaagatg
     4741 gcgatacacc aagtctggcc gcgaatttct tagcatcacc aacccttaat gatttaataa
     4801 actcttttaa tgtcatacct tcctcggttt agtgtttttt tgcgagttta gtgtttaata
     4861 aaccattaag tcaagtattt gcttgtttag tgattactaa agataattac cacatgcaaa
     4921 aaaaagaaat tcgccgttta cgtctcaagg agtggtttaa agataaaact ctgccaccca
     4981 aagagaagag ctacctatct caactaatga gtgggagagc ctcgtttgga gaaaaggctg
     5041 ccagaagaat agagcaaaca tacgggatgc cggaagggta tctggatgcg gaatacgcag
     5101 aacaaccggg gggttctcca ccacatgcag ggttaacgtc taatcaactg gaattattgc
     5161 agattttttc agccttccct gaggatgagc aacgccagat aatcagcgag ttaaagcaga
     5221 aaaaagaatc aatggaagat ctcatagcga gatggattgc ggcgcaaaaa tgccgccgcg
     5281 cctgagttat aaaaccggag gaaacatgaa tagagccctt tcaccaatgg tttctgaatt
     5341 tgaaaccatt gaacaagaaa acagttacaa cgaatggctg cgtgcgaaag tagcaacgag
     5401 ccttgcagat ccgcgcccag caattcccca tgacgaagtt gagcgcagaa tggcagaacg
     5461 ctttgctaag atgcgcaagg aacggagcaa gcagtaaaat gttacccgtg ttatggcttg
     5521 aaagcgcaga taccgaccta gatgatataa ctagttatat tgctcgtttc gacatagatg
     5581 cggctgaacg cttatggcag cgattaaggg gttgtgtgct gccgttatcc gaacatccgt
     5641 atttataccc accaagcgac agagtacctg gcttgcgtga gattgtagcc caccctaact
     5701 atataattct ataccgcgta acaacatcaa gcgttgaagt agtaaacgtg atccacgcaa
     5761 gacgccagtt tccctaactt tcactaccaa tagaaacata acaaccgcaa cgactttatc
     5821 aaaagcgttg tgtttgttat gtcccgcggt ttagttttta cttgacttaa gtttaatgtt
     5881 tattaaacta aaaataccaa cccaccccgc cccacagaac gcagggaaat acttcgagtt
     5941 acccggcagt ggtcaggggt taagtagcca gcccgaggcg tatgaacatg acggcagggt
     6001 tcaactttaa taactatgca gcagtttttt gttccgctac cccggcgtta aggggaaaca
     6061 gagggtttct cagtgggcga agtcaaacat cagaatggaa ggcatcccgg gatcggcaaa
     6121 gaagcagcaa tggcgcttta tattgacatc agcgccattg caggacaggt aagagttatc
     6181 agagcggtaa ctaagcggta tgcgccttta cttcagaaag tctctggtga gtgcaccgaa
     6241 gatattgtca acgatttcgt catcgaactg cgaggactca tcttcagtta caaggtgacc
     6301 acaatttttg cagatggctc ccgcgaaact gtcagagccc tgcggcttaa aggatgtgtc
     6361 aaagacttcg ccaccacatt ctgggcaaga aaacttgatt gtattcataa ccaatttcct
     6421 ctcgagtaac agacccctca gaggatacca cctcgcctga cgtggttaaa agcaggcaac
     6481 gctaaccaca aggagccgac atgcagaaac gagaacccgt catcatcgcg ccagactata
     6541 ccgatgatga actttatgag tggatgcacc agaaaattaa tgcagcgcag gatctgaaat
     6601 gggccaatga agccagggct aagcaggctg aaaatctgtc cgctctggag caggatatca
     6661 ccaatctgga aaaagcagcg gcattaagca ttgccagaat gattacatac ccgcgttaat
     6721 agctaaccaa cgaagctaag gttggtaatt aaggagttct ccacgggtga ggtggagtgc
     6781 gtgcgccgga cacgggtgag catacggcac tgacagttta ctgaaaggat atttccctga
     6841 aaagtcagac cataacgcga aagcgcacgg cgaggtagct ggttcataga tagcctgtcg
     6901 ttaaattttc gtcgaccgtg cgcttccggt tgtggcaatc cgcgaaatgg cgcggcggta
     6961 agtatggagg ggttattcct tcccccgttg aggacaccgg gttgtcaggt tgaccatacg
     7021 cttaagtgac aaccccgctg caacgccctc tgttatcaat tttctggtga cgtttggcgg
     7081 tatcagtttt actccgtgac tgctctgccg ccctttttaa agtgaatttt gtgatgcggt
     7141 gaatgcggct gagcgcacgc ggaacagtta aaaccaaaaa cagtgttatg gggggattct
     7201 ctgtatccgg cgttaattgt taactggtta acgtcacctg gaggcaccag gcaccgcatc
     7261 acaaaattca ttgttgagga cgcgataatg gaaacgttat taccaaacgt taatacgtct
     7321 gaaggttgtt ttgaaattgg tgtcactatc agtaaccctg tatttactga agatgccatt
     7381 aacaagagaa aacacgaacg ggagctatta aataaaatat gcattctttc aatgctggcc
     7441 cgtttacgtc cgatacaaaa aggatgctgg caatgaatac agcatttgca cttgttctga
     7501 cagtttttct tgtttccgga gagccagttg atattgcagt cagtgttcac aggacaatgc
     7561 aggagtgtgt gactgcagca accgaacaga aaattcccgg taactgttac ccggtcgata
     7621 aagttattca ccaggataat aacgaaatcc cggcaggtct ttaaaacagt tccgtaataa
     7681 acatccgatt tcattcttat atgccagcaa tggcagggat ttgttcaccc ttaaatctgt
     7741 aatgaggtaa aacaaaatga gtaaagtctt tatttgcgcc gccattccgg acgaacaggc
     7801 aataaaggaa gaaggtgccg tcgctgtagc cactgccatt gaagccggtg atgaacgtcg
     7861 cgcccgcgca aaatttcact ggcaattcct ggaacattat ccggctgctc aggactgcgc
     7921 ttataaattt cttgtttgcg aggataaacc cggtataccc cgccctgccc tcgattcctg
     7981 ggatgctgaa tatatgcagg aaaaccgctg ggatgaggcg tctgcttcct ttgtcccggt
     8041 cgagactgaa tccgatccga tgaacgtcac ttttgacaac ctggcccctg aagtacagaa
     8101 cgctgtcatg gttaagttcg acacatgtga aaacatcact gttgatatgg tgattagcgc
     8161 acaggaattg ttgcaggaag acatggcaac attcgacggc catatcgttg aagcgttgat
     8221 gaaaatgcca gaagttaacg ccatgtatcc ggagcttaag ctgcatgcca tcgggtgggt
     8281 taagcataaa tgtaagcctg gtgccaaatg gcccgaaatt caggcagaga tgcgcatctg
     8341 gaaaaaacgt cgcgaaggtg aacgcaagga agccggaaaa tacacgtctg ttgttgatct
     8401 cgcccgcgcc agagccaatc aacagcacac tgaaaattca acaggaaaaa tcaacccggt
     8461 cattgctgcc attcatcgcg aatacaagca gacatggaaa acactggatg acgaactggc
     8521 ctacgctctc tggcctggtg atgtggatgc cggaaacatt gacggcagca tccatcgctg
     8581 ggcaaaaaat gaagttatcg acaacggccg cgaagactgg aagcgtatct cggcatcaat
     8641 gcgcaaacag cctgatgccc ttcgctacga ccgccagact atttttggcc ttgtccgtga
     8701 acgtccgatc gacattcaca aagaccctgt ggcactgaac aaatacatta ctgaatacct
     8761 gactacaaag ggcgtgtttg aagatgaagg aacaaatcag agcgcaactg atactctctc
     8821 gtcgccagta ccagaa
//
LOCUS       DABSYT020000149         8770 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.149, whole genome shotgun sequence.
ACCESSION   DABSYT020000149 DABSYT020000000
VERSION     DABSYT020000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.149"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..111
                     /locus_tag="ID725_RS22355"
                     /old_locus_tag="pgaptmp_004471"
     CDS             <1..111
                     /locus_tag="ID725_RS22355"
                     /old_locus_tag="pgaptmp_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852930.1"
                     /translation="NKFEIACDSDGFYIFKALIQLRRFTGFKNVRLYQLH"
     gene            154..714
                     /locus_tag="ID725_RS22360"
                     /old_locus_tag="pgaptmp_004472"
     CDS             154..714
                     /locus_tag="ID725_RS22360"
                     /old_locus_tag="pgaptmp_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7852931.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLMPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            800..1501
                     /locus_tag="ID725_RS22365"
                     /old_locus_tag="pgaptmp_004473"
     CDS             800..1501
                     /locus_tag="ID725_RS22365"
                     /old_locus_tag="pgaptmp_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGV7852932.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1554..4071
                     /locus_tag="ID725_RS22370"
                     /old_locus_tag="pgaptmp_004474"
                     /pseudo
     CDS             1554..4071
                     /locus_tag="ID725_RS22370"
                     /old_locus_tag="pgaptmp_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            4087..5421
                     /locus_tag="ID725_RS22375"
                     /old_locus_tag="pgaptmp_004475"
     CDS             4087..5421
                     /locus_tag="ID725_RS22375"
                     /old_locus_tag="pgaptmp_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7852933.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWANCTNTQIGQTEDGRTALI
                     EFGKINMTDTYFAPAGSLLATTVVPPTNYTSGGATGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFYDAGGPDGLSDVYATWFAFVGLKQTMAGVTLGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTSATTSWCGNNNTDSSGVGFAKPSGTIYNCVQPNAY
                     IQLSGTSGILFGHDEPGEDSSVHWDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAHPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5418..6107
                     /locus_tag="ID725_RS22380"
                     /old_locus_tag="pgaptmp_004476"
     CDS             5418..6107
                     /locus_tag="ID725_RS22380"
                     /old_locus_tag="pgaptmp_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGV7852934.1"
                     /translation="MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6253..7035)
                     /locus_tag="ID725_RS22385"
                     /old_locus_tag="pgaptmp_004477"
     CDS             complement(6253..7035)
                     /locus_tag="ID725_RS22385"
                     /old_locus_tag="pgaptmp_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGV7852935.1"
                     /translation="MSFSIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANL
                     LLVDIKEPDNRYLKHLTHEGVEVEFMTIDITKPDASCTIINRCLERFGQLDILVNNAG
                     VCNINRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGR
                     WSPGYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKG
                     RIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
     gene            7723..8535
                     /locus_tag="ID725_RS22390"
                     /old_locus_tag="pgaptmp_004478"
     CDS             7723..8535
                     /locus_tag="ID725_RS22390"
                     /old_locus_tag="pgaptmp_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGV7852936.1"
                     /translation="MIYIINDEVRFNSSSNKLTSLLTSEEVILTYPAGKCLKLLLDNA
                     YSVVSHNQFFEKVWSDSSADVATNTLYQNISMIRRSFREISSATIFNDVIRTVPRKGF
                     KINEEISITVLDNNHNENESVESEENGTHTENINKNHIAKGIFTTKNTLLIVCFFAAW
                     GIVYISSYFFDMAFQSKSHTIKKIDAFSNYAQHPTQEGCSYFYDNKYFNSVDMLSPII
                     EKLQKMEINCKIHPYIYIATNDSMSGFYAFTCSQMLNSESPNNCNSLYYKAE"
     gene            8540..>8770
                     /locus_tag="ID725_RS22395"
                     /old_locus_tag="pgaptmp_004479"
     CDS             8540..>8770
                     /locus_tag="ID725_RS22395"
                     /old_locus_tag="pgaptmp_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fidL"
                     /protein_id="HGV7852937.1"
                     /translation="MTQRYFLFAGIILCAFIAAILSHIAFHHANEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTV"
BASE COUNT         2510 a         2024 c         1928 g         2308 t
ORIGIN      
        1 aacaagtttg aaattgcttg tgatagtgat ggcttttaca ttttcaaggc acttatacag
       61 ttaaggagat ttaccggttt taaaaacgtc agattgtatc aattgcatta attaactcgt
      121 tctatattaa ctctaaattt caaggatatt aatatgtttt ctactttcaa aaaagcagct
      181 ctgctggcag ctattgcatt acctttttca actatggctg cgcctacagt cacttttcag
      241 ggtgaagtaa ccgatcagac ctgttccgta aatatcaacg gtcaaaccaa ttcagtagta
      301 ttgatgccga ccgtagccat ggctgacttc ggtgcaactt tagctgatgg tcagagcgca
      361 ggccagacgc cgtttacggt ttctgtgtct aactgccagg ctccaactgg tgcagatcag
      421 gcaatcaaca ccaccttcct gggctacgac gttgacgcta gcacgggtgt tatgggaaac
      481 cgtgatacca gcagcgatgc ggcgaaaggc tttggcattc agttaatgga ttccagcact
      541 tctggtaacc cagtaactct ggctggcgcg actaacgtac cgggtctgac cctgaaagtt
      601 ggcgataccg aagccagcta cgacttcggt gcgcgttact tcgttatcga tagcgctgct
      661 gccactgccg gtaaaattac cgctgtcgca gaatacaccc tgagctacct ctaattaatc
      721 tactggctgg cagtaaatgc cagccagtat ttcttcgcac aattaatagt gatttttcat
      781 ggacatggaa aagataagaa tgaacatttt tgcatattta ctggtacttg tattttccat
      841 gagcatgagc agcagcgcgt ttgccagcgt ggtaatgacc ggaacccgta ttattttccc
      901 tggtgacgca aaggaaaaaa ccatccagtt gcgaaatacc agcgatcagc cctatatcat
      961 taatatccat gttgaggatg aacgtggttc tgacaagaat gtaccgttta tgccaacccc
     1021 gcagacattt cgcatggaag ctgccgcagg tcaggcgtta cgcctgctct acactggtaa
     1081 taatttaccg caggatcgcg agtctgtttt ctggtttagt ttcagtcaac taccttatct
     1141 gaataagaat gataaaagtc agaaccagct catcctggcc ctgactaatc gagtcaaaat
     1201 tttctatcgt cccagctcga ttgtcggtaa atccagtgac gcacccaaaa acctgactta
     1261 ccaggtaaaa cagaaccgca ttgaagtgac gaatcccacg ggctattacg tcacaattcg
     1321 cgccgctgaa ctgcttaata atggtaaaaa agtccccctc gcgaattcgg taatgattgc
     1381 tcctcaaagc acaactgaat ggacactacc ctctggcatc agtgtcgctc ccggtgcgca
     1441 gatccattta gtgaccgtca acgactatgg cgtaaatgtt acgtctgagc atgccttata
     1501 acctctttct ccttttttga gcggtatcct ttttcacgca cgagtacgtc gagatgaata
     1561 aatccgttgt gtcaatttct gcggcaatgt tggttttact ttgccaaccg gtcatgggga
     1621 gcgaaatctc acccgcaaca ccgtcagatg aagacaacta cacctttgac ccgcaactct
     1681 tccgcggcag cagatttagt cagtcgtcat tagcaaaact gacaacacgt gagtctgttg
     1741 caccgggcaa ttataaaatg gatatctaca ccaacaataa gttgtcaggc agttggaatg
     1801 tcacgtttaa agaagccgct gatggtcgcg ttctgccctg cctgacgcct gaagtcgcgg
     1861 acgcgatagg cctcaaaaca ggggaagata agggggaaaa agatcctgtc tgtacgtttg
     1921 ctaaggaact cgctcccggc atcaccagcc agacacagtt gtcacaattg cgcctggact
     1981 tatcggtgcc acagagtcaa ttgattagtc gccctcgcgg ctatgttccc cccagcgagc
     2041 tggataccgg agcatcgctg gcgttcatga attatattgc caactattac aacgttgcct
     2101 attcagggca gaatgctcat agccagcgtt cgctatgggc atcatttaat ggtggcatca
     2161 accttggtgc ctggcaatat cgtcagttat ccaacatgac ctgggataat gacaaaggga
     2221 atcagtggaa caatattcgt agctatttgc aacgcccgct gcccgccata aatagccagt
     2281 taatgatggg gcagcttatc accagcggaa gatttttctc tggactcagt tatcacggcg
     2341 ttagtctcgc gaccgatgaa cgtatgctgc cggactccat gcgcggctat gcgccgacta
     2401 ttcgcggcgt ggccgcaaca aacgccagag tctcggtaat gcaaaacggt catgaaatat
     2461 atcagaccac cgtggctcct ggccctttcg agataaacga cctatacccc accagctaca
     2521 gcggcgatct ggatgtcacc gttacggaag ctaacggcgc agtcagtcgt ttcagtgtcc
     2581 ccttttcagc cgtaccagaa tcgatgcgtc caggaacttc ccgttataac gtggaagtag
     2641 gtaaaacgca ggatagtggt gatgactcga tgtttggtga ccttacctgg cagcacggga
     2701 tgactaatac gctgacattt aacagtggtt cgcgtatcgc tgatggctac caggcgctga
     2761 tgctgggcgg agtctatggc agttcgctgg gggcatttgg ggcaaacctc acttggtccc
     2821 atgcgcgtgt tcccgaaagc gaagcgcaga gtggttggat gtcgcaatta acctggagta
     2881 aaactttcca gcctacttca accaccgtct ccctggcagg ttatcgatac tctaccagcg
     2941 gctatcgtga tctggctgat gtgctgggag agcgtcatgc tgccagcaat aaacagtcat
     3001 gggactccag ccagtggcgt caacagtcgc gcttcgatct tacgttaagt cagagccttg
     3061 cgaattacgg caacctgttt gtgtcaggtt caacacagaa ctaccgtggc ggcaagagcc
     3121 gtgatacaca gcttcagtta ggttacagca atagctttag ccatggcatc agtatgaacc
     3181 gtcggacgcc aaagaatggg cggctataaa gacaattctg atgatatgca gacggtaaca
     3241 tccctttcat tctcattccc acttggcggc aatggacctc gtgtaccaag tcttagcaac
     3301 agctggaccc attcaactga cggtagctcg caattacaaa gctcgctaac cggaatgctt
     3361 gatgaagcac agaccaccaa ctacagcctg aacgtcatgc gcgatcaaca atataagcag
     3421 acgacgctta gcggaaacat gcaaaaacgt ttttcacaaa ctaccgtcgg attgaacgca
     3481 tcgaagggcc aggattactg gcaggcttca ggtaacgtac aaggcgcgat ggctgtgcat
     3541 ggtggcggca ttactttcgg accttatctg ggtgaaacgt tcgccctggt cgaagctaaa
     3601 ggcgcagaag gtgcaaaagt ctataactcc agtcagctgg aaattaatga cagtggctat
     3661 gcgcttgttc cggcagtaac gccctatcgc tacaaccgta tatctctcga tccacaagga
     3721 atggatggcg atgccgagtt ggtcgacagt gaaagacagg tagcaccggt tgcgggtgcg
     3781 gcggtgaaag taattttccg tacccgtcct ggtaaagcgt tgctgattaa atcccgcatg
     3841 gcagatggtt cggaactgcc aatgggagcc gatgtgctgg atgagaataa tacagtcgtc
     3901 ggtatagccg gtcagggggg gcaaatttac ctccgcacag aacagacaaa aggccacttg
     3961 tcagttcgct ggggtgaagg tgctaacgat agctgccaat tgccctttga tatcagcggg
     4021 aaggacagca atagccctat catccgcctg aatgaaacct gtcagtcttg atacagagaa
     4081 aacccgatgt ctaaatcgac ttttttacac attctgatta gctccatcat attggtggca
     4141 ctaatacaat catccgcttg ggctaactgt acaaacacac agataggtca aactgaagat
     4201 ggtcggacag cgcttattga gttcggaaaa attaatatga ccgacaccta ttttgcgcca
     4261 gcaggttcac tcctcgcgac aacggtcgta cctcccacta actacacatc aggtggcgcg
     4321 acaggaagct ccgtattgtg ggaatgtgat gcaacagatt tgccaaacat ctattttctg
     4381 gtcgctacca atggcgacga ccgcgtgggg ggcttttacg atgcaggcgg gcctgatggt
     4441 ctgagtgatg tctatgccac ctggttcgct tttgtcggtc tcaagcagac catggcgggc
     4501 gtgacgcttg gtcgttactg gaagaaagtg cccatcacca gttatgccac tcagggaact
     4561 aaaatccaga ttcgcttaca ggatatccct cctcttcatg ctgagcttta tcgcatcagt
     4621 acgctacctg atacatcagc aacaacaagt tggtgcggta ataataatac agatagtagt
     4681 ggagtcggat tcgcaaaacc ttccggtaca atctataact gtgttcagcc caatgcctat
     4741 attcagcttt ccggtaccag cggcatttta tttggtcatg atgagcccgg cgaagatagt
     4801 tctgttcatt gggatttctg gggtgctgat aatggttttg gttacggaat gcgttcggcc
     4861 aatcgactct acaacaatgc cacctgcgtt gcccgcagcg ccacgccgtt agtattgctg
     4921 ccgacaattg cagaagcaca actgaatgcg ggcatggaaa gtaccggtaa ttttaatgtc
     4981 cgcgtcgagt gtagtaactc ggttcaatca gggattagcg atactcagac agcattagga
     5041 atccaggtgt ctgaaggtgc atatacagcg gcgcaaaaac tggggattat caatagcaac
     5101 ggcggcgtca gcgccctggt ctctgataat tatgacgcag cagagatggc aaagggcgtt
     5161 gggatctaca tttctaacag tgctcacccc gatacggcga tgacgctggt tggtcaaccg
     5221 ggcatcgcga agttaacccc cggaggaaat gcagcggggt ggtatcctgt atttgaaggg
     5281 gcaacattag aaggtgcgac tcaccccgga tactccagct atagttactc ttttatcgcc
     5341 cggttgaaga aactgccaaa tcagacagtc agtgcgggaa aagtgcgggc aacggcttat
     5401 atattggtga aaatgcaatg aaatccgttt tgctgctctt aaccatcttg ttcgtacagt
     5461 cagtggaagc cggcatcatg ccggcttctt cacggattat ttatcgcgat ggcgatcggg
     5521 aaaagacact gatgctggtc aataccaatg actatccggt actggtgcaa agttgggtag
     5581 ataatggtga aggttcccca gaagctgctt ctcccccctt tgtggtgctg ccaccggttt
     5641 tccgcctggc tgcgagacga acccaggggt tgcggattat ctataatcat gacccacttc
     5701 cccaggatcg cgaatctgca ttctggttaa atctttatga aatgccgcca gaaactcccc
     5761 atcaagatcc cacacattta acgctggcaa tgaatacgca acttaaaatc ttttacaggc
     5821 caaaatctgt aactatgacg ccccaagaag ctatgcgtaa aataagttgg catctgggta
     5881 acgacggcag cagtagctat attgagctta ataacccaac accactgaat atctctttca
     5941 cctccatcag cattggggct acaaaggtgc agcaagaaag cgatatgatg ataaaaccct
     6001 tcagtcaacg acggtactgg ttagcaagta ataacgttag tggtgtggtt gcggcgacat
     6061 atctcgatga taatggtgca cagataaaac aacaattagc gcaataatgc tctgagtggt
     6121 atgtaaactc atcgcgatgg ttcaacgcta acgaataacc actataccca cctgaagtac
     6181 cggaagtgga gaaatgacaa aatctctcca ctccattttc cagtcaaaac acctcaacgc
     6241 agccaaaaat cgttaacgaa ttgaatatcc accatcaatg actaatagtt gtccattgac
     6301 ataatctgaa gcggcactgg cgaggaacac cattgcgccc ataagatcct gtgcccttcc
     6361 ccagcgttga gccggtatac gcccttttat taactctttt attttagggt tattataaat
     6421 aatagcggtc atttcactca cataatatcc tggggcgata ccattaatct gaatattata
     6481 ttccgcaaaa tcatcggcat acgctttggt cagccccgct aaagcatgtt tagtcgccgc
     6541 ataccctggc gaccaacgtc ccccatgaaa tgacaatacg gaacacatat taataatttt
     6601 cccttttctt tgcgggacaa atatattcaa tgcggcctgg ctcatatcga atgcagcatt
     6661 caggttgaga ttaatcatcg gatcccagtc gtttcgattg aaatcgatag gacgattaat
     6721 gttacacacg cccgcgttat tgaccagaat atctaactga ccgaaacgct ccagacacct
     6781 attaataatg gtgcatgatg catcaggttt tgttatgtct attgtcataa actcaacttc
     6841 aacgccttca tgagttaaat gcttcaaata tctattatca ggctctttga tgtcaaccaa
     6901 caatagatta gcccctgctt tggccagagc cgagcataag cctctcccca gctcacccgc
     6961 ggccccagtt acgatggcca ccttgttatg taatgaaaaa aaatccaatg aaaaatcttc
     7021 aatagaaaag ctcatatcac acattgctcc ttatgagtaa gtaatattta agggataatt
     7081 taagtaattt ttagatttca ataaaaaaat ataaacatta aaactcagca tgaataataa
     7141 acatcaaatc ttatatacat ttaagtagtg atgtatcacg taaaaatata gtcatcatag
     7201 atgttatgca gtacgcacaa aaaaacagac tggatttgct tgctgatcta taatctttat
     7261 ctaaattact attggcgctt tggataataa cataagcata tattacctcc tagcaataat
     7321 agatataacc tcatatcaag gtgtcactgg taaataatcg gataaaagac agtaccggtc
     7381 ggcacataga gcttcttcta tttcaccaga agtgcacagc agggaactac caatcacacg
     7441 gttagtgtca tgggtggagg atgcgtcccg ttctttccct ataacgggga tacgcagttt
     7501 ttatcagtac atgcagaaaa acccctaatt cacatcactg cagaaagcat ttaatagaaa
     7561 tatacatcgt ttaagaattt caattaaatc aagattattc ttaaaaatca gataattcag
     7621 atttgcattt ttaggaaatt atactatagt gcacttaatg ttcttgcctt tattttctga
     7681 gtaatactgg atttgtaata cacaactaag gtataaaatg taatgatcta cattataaat
     7741 gatgaagtaa gattcaactc atcatctaat aagttaacat cactccttac atcagaggaa
     7801 gtgatactca cttatcctgc gggaaaatgc ttaaagttac ttttagataa cgcttatagt
     7861 gtcgtgtcac acaatcaatt ctttgaaaaa gtctggagcg atagcagtgc cgatgtcgca
     7921 accaacactt tatatcaaaa tatatcgatg ataagacgtt cttttcgtga aatcagctca
     7981 gcgacgatat ttaatgatgt tatcagaacg gtgccccgta aaggcttcaa aatcaatgaa
     8041 gaaatcagca taactgttct cgacaataat cacaatgaga atgaaagtgt tgagagtgaa
     8101 gagaatggta cccacactga aaatataaat aaaaatcaca ttgcaaaagg tatatttacc
     8161 acaaagaata cattacttat agtttgtttt tttgcggcat gggggatagt gtatatatct
     8221 tcttattttt tcgatatggc ttttcaaagc aaatcccaca ctataaaaaa aatagatgct
     8281 ttctcaaatt atgctcaaca ccctactcag gaaggatgca gttatttcta tgataataaa
     8341 tactttaatt ccgtagacat gttatcaccg attattgaaa aattgcagaa gatggaaatt
     8401 aactgcaaaa tacatcctta catttacatt gcaacaaatg atagcatgag cggattttat
     8461 gctttcacct gtagtcagat gttaaactct gaatcaccaa ataattgcaa ctctctctat
     8521 tataaagcgg agtaatcata tgactcaaag atattttctg tttgcgggaa taattttatg
     8581 tgcttttatt gcagcaattc tgagccatat tgcgtttcat catgccaatg aaccagcaga
     8641 gcaaaacata tcatgtaatg cccatgttat taatttcacc gatcatacaa aaatggatca
     8701 atatttttcg ttaaatatga tttctgaaaa taacacagga aacatgtatc tcacgggcgc
     8761 atacacggta
//
LOCUS       DABSYT020000150         8750 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.150, whole genome shotgun sequence.
ACCESSION   DABSYT020000150 DABSYT020000000
VERSION     DABSYT020000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.150"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..1428
                     /gene="citT"
                     /locus_tag="ID725_RS22400"
                     /old_locus_tag="pgaptmp_004480"
     CDS             <1..1428
                     /gene="citT"
                     /locus_tag="ID725_RS22400"
                     /old_locus_tag="pgaptmp_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGV7852938.1"
                     /translation="APLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPAT
                     AISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFA
                     LGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIK
                     NLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQ
                     ISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREW
                     TLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLA
                     TLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTAT
                     MLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWR
                     LGAIFGVIYISMLLLVGWPILAMWN"
     gene            1542..2348
                     /gene="rna"
                     /locus_tag="ID725_RS22405"
                     /old_locus_tag="pgaptmp_004481"
     CDS             1542..2348
                     /gene="rna"
                     /locus_tag="ID725_RS22405"
                     /old_locus_tag="pgaptmp_004481"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGV7852939.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            2578..2988
                     /gene="rnk"
                     /locus_tag="ID725_RS22410"
                     /old_locus_tag="pgaptmp_004482"
     CDS             2578..2988
                     /gene="rnk"
                     /locus_tag="ID725_RS22410"
                     /old_locus_tag="pgaptmp_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGV7852940.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            3126..3215
                     /gene="yldA"
                     /locus_tag="ID725_RS22415"
                     /old_locus_tag="pgaptmp_004483"
     CDS             3126..3215
                     /gene="yldA"
                     /locus_tag="ID725_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGV7852941.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(3219..4457)
                     /gene="ybdR"
                     /locus_tag="ID725_RS22420"
                     /old_locus_tag="pgaptmp_004484"
     CDS             complement(3219..4457)
                     /gene="ybdR"
                     /locus_tag="ID725_RS22420"
                     /old_locus_tag="pgaptmp_004484"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGV7852942.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            4678..5106
                     /gene="uspG"
                     /locus_tag="ID725_RS22425"
                     /old_locus_tag="pgaptmp_004485"
     CDS             4678..5106
                     /gene="uspG"
                     /locus_tag="ID725_RS22425"
                     /old_locus_tag="pgaptmp_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGV7852943.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(5227..6792)
                     /gene="ahpF"
                     /locus_tag="ID725_RS22430"
                     /old_locus_tag="pgaptmp_004486"
     CDS             complement(5227..6792)
                     /gene="ahpF"
                     /locus_tag="ID725_RS22430"
                     /old_locus_tag="pgaptmp_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGV7852944.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(6921..7484)
                     /gene="ahpC"
                     /locus_tag="ID725_RS22435"
                     /old_locus_tag="pgaptmp_004487"
     CDS             complement(6921..7484)
                     /gene="ahpC"
                     /locus_tag="ID725_RS22435"
                     /old_locus_tag="pgaptmp_004487"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGV7852945.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            7856..8602
                     /gene="dsbG"
                     /locus_tag="ID725_RS22440"
                     /old_locus_tag="pgaptmp_004488"
     CDS             7856..8602
                     /gene="dsbG"
                     /locus_tag="ID725_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGV7852946.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         2126 a         2219 c         2122 g         2283 t
ORIGIN      
        1 gccccactgg tggtgatggg tgtcatgttt cttatccctg tccccgacgg tatgccgccg
       61 caggcatggc attacttcgc tgtgtttgtg gcaatgattg tcggcatgat cctcgagcca
      121 attccggcaa cagcgatcag ttttattgcg gttactattt gcgttattgg cagtaattac
      181 ctgctctttg atgccaaaga attagctgac ccagcgttta atgcgcaaaa acaggcgctg
      241 aaatggggcc tggctggttt ttccagcact acggtatggc tggtatttgg cgcatttatt
      301 tttgcattag ggtatgaagt ttccgggtta ggtcgtcgca ttgccctttt cctggtgaaa
      361 ttcatgggca aacgcacgct gacgttgggt tatgcgattg tcattatcga cattctgctg
      421 gcaccgttta caccgtccaa caccgcgcgt accgggggta cggtttttcc ggtcattaaa
      481 aacctgccgc cgttgtttaa atcattcccg aacgatccgt ccgcgcgtcg tattggcggc
      541 tatttgatgt ggatgatggt cattagtacc agtctgagtt cgtccatgtt tgtcaccggt
      601 gcggcaccaa acgtgctggg tctggagttc gtcagcaaaa ttgccggtat ccagattagc
      661 tggttgcagt ggttcctctg cttcctgccg gttggggtta tcttgcttat cattgcgccg
      721 tggctttcct acgtgctgta caaaccggaa atcacacaca gtgaagaagt ggcaacctgg
      781 gcgggtgatg aactaaaaac catgggtgcg ctgacacgca gagagtggac gctgattggc
      841 cttgtattgc tcagcttagg tttgtgggta tttggcagtg aagtcattaa tgctactgcg
      901 gttggtctgc tggcagtttc gctaatgctg gctctgcacg ttgtgccgtg gaaagacatt
      961 acccgctata acagcgcatg gaacacgctg gtcaacctgg caactctggt tgtgatggct
     1021 aacggcctga ctcgttctgg ttttattgac tggttcgccg gtaccatgag tacgcacctg
     1081 gaaggattct caccaaacgc aacggtgatt gtactggttc tggtgttcta ctttgcacac
     1141 tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc tgccggttat tctggccgtc
     1201 ggtaaaggta ttccgggcgt accaatggaa caactgtgta tcctgctggt gctgtctatc
     1261 ggtatcatgg gctgtctgac gccgtatgca accggtcctg gggtgattat ttacggctgt
     1321 ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa tcttcggggt gatttacatc
     1381 tctatgttgc tgttggttgg ctggccgatt ctcgccatgt ggaactaata cgttcgccaa
     1441 acgctgaata aatcatcacg cccgccaggt tagcccacct ggcgggcttt tttatgattt
     1501 aatagatagt attcagtgta tgtatgagtt ccacacccat tatgaaagca ttctggcgta
     1561 acgccgcgtt gctcgcggtt tctctgcttc ccttctcttc tgccaacgcc gtagcgttgc
     1621 aggcaaaaca gtatggcgat tttgatcgct atgtcctggc cctctcctgg caaaccggat
     1681 tttgccagag tcaacacgat cgaaatcgta acgaacgaga tgaatgtcgc ctgcaaaccg
     1741 aaacgaccaa caaagctgat tttctgaccg tacatggtct gtggccagga ttgcctaaat
     1801 cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg cttcggttgc gctactcgcc
     1861 caatcccgaa tctaccagaa gcgcgcgcca gccggatgtg ttcatcgccg gaaaccggat
     1921 tatcactgga aacggccgct aaactaagtg aagtcatgcc aggagctggc ggacgttcct
     1981 gcctggaacg ctacgaatat gccaaacacg gtgcctgctt tggttttgat ccggacgcat
     2041 acttcggtac gatggtacgc ctgaatcaag agataaaaga gagcgaagcc ggaaaattcc
     2101 ttgcggataa ttacggtaaa acagtgagcc gccgtgactt tgacgccgcc tttgccaaaa
     2161 gctggggaaa agagaacgtg aaagcagtta agctaacgtg ccagggtaac cctgcgtatt
     2221 tgactgaaat tcaggtctcg atcaaagctg acgccatcaa cgctccgctt tctgcaaact
     2281 cattcttgcc acaaccacac ccaggtaact gtggcaaaac ctttgtgatt gataaagcgg
     2341 gttattaatc ctctgattta caaaacacgc taattcgtct ttcataaacc atgacgatta
     2401 agaattatac ataatgcgct gcattgagga attccctgaa tgtgacgcaa atcacttcaa
     2461 agagtgagca acagtcagta tcatcatgtg agttaaaccc tcgccgcctg acggtgaggg
     2521 ttttcttttg ggtatgttta tctgcgacac tcgcagtacc gacgacatgg agtaaaaatg
     2581 tccagaccaa ctatcatcat taacgacctg gatgccgaac gcatcgatat tctgctggag
     2641 caacccgcct atgctggttt gccaatcgcc gacgcgttaa acgcagagtt ggatcgcgcc
     2701 caaatgtgtt cgccagaaga gatgccacac gacgtggtga caatgaacag ccgggttaaa
     2761 ttccgcaatc ttagcgatgg cgaagtgcgt gtgcgcacgc tggtgtatcc ggcaaaaatg
     2821 accgatagca atactcagct ttccgttatg gctccggtag gtgccgcact gctgggactg
     2881 cgcgttggcg attccattca ctgggaactt ccgggcggcg ttgcaaccca ccttgaagtg
     2941 ctggaactcg aataccagcc agaagctgct ggcgactacc tgctttaatc ccatctgtac
     3001 aagcgataac tcgctttgcc cagaggatgc actcgcatcc tctttatgca aaatcagcaa
     3061 tttttcctgg ttctcactgt tcacttttgc tgcaaaatta ttccgtttcg tttgtgggaa
     3121 taaccatggc agaagcattt tacatcctga taggatttct gattatggcg gcgattatcg
     3181 tcatggctgt cctttacctc gaaaaccact cctgacgctc atattgttcc ccccggcatc
     3241 gcattcacca gacctgaaac cgccttctgc gccgcctctg cgctttgtgc accgggtacc
     3301 agaatcacct tacggcactc ctcttcacgc ttttcaaaga tctcatatcc ccgggcggcc
     3361 tcttcaaacg gcatatagtg ggtaacaatt tcttctggtt tcagtaatcc tttctcaatt
     3421 aacggtaata attctcccag ccatgcgtga acgtgggtct gtcccatttt aaacgtcaac
     3481 cctttatcaa aggcgtcgcc aaacaggaaa ccgtgaataa atccagcgta gacgcccggt
     3541 acgctaacaa tgccgccacg cctgaccgcc gcaatacact gacgcaacgc tttaccgctg
     3601 ctgccctcca gtttcaggtt agtcagcacc gtttccgtgg tgctgccttt cgcttcaaaa
     3661 ccgacggcgt ctattactgc atccacgccc cggtgacctg ccgtttgttc aataattgac
     3721 tgtgccggat cgctatcttc atcgaaatta atcgggatcg cgccgtagcg gtcagcggcg
     3781 aaacgcaagc ggtagggatg atgatcaaca acaaaaatct gttccgcacc gagcaaccgt
     3841 gcacaggcga ttgtcaacaa tcccacagga ccggcaccat agacagcaac gcttgaacct
     3901 tgttggatct gcgcattttt tgctgcctgc catgccgttg gcagaatatc agaaaggaaa
     3961 agggctttat catctgaaag caaaggcggt actttaaacg gccccacatt ccctttaggg
     4021 acacggacat actccgcctg tccaccagga acgccaccat acaggtgact ataaccaaac
     4081 aatgccgcgg gagctggtat ctgtttttta ttgagcgcag cgcctttacc cgcattggta
     4141 ttttcgcagg cagcatattg ctgcagtcga cagaaaaaac agtcgccaca agcaatgacg
     4201 aacggaatta ccacccggtc gcctttttgc aaatttttta catcctttcc ggtttcaact
     4261 acttccccca taaattcatg accaaaaata tcgccatgtt taacctgggg tattttgcct
     4321 cgataaagat ggaggtcaga gccacagatc gccgttgccg ttatacgcag aataatatca
     4381 tctgcctgtt caatgcccgg atcgggaaca ttttctacct gaacgtgatg tgggccgtga
     4441 taagtcaatg ctttcatgca acctccagag acagttcaaa aaagagtcag tctttaaggg
     4501 tagctatcac agcgggaaaa cgggcggtac cgcaggtttt gggagaattc ctggtgttca
     4561 gtgagaatat attcatcaca gaaacagttc gtaaggaaaa agaaccagac aaatatcagg
     4621 gataaatatt ataatcatgg cgaatgattt gttcatgatt aacagggaga aagggttatg
     4681 tataagacaa tcattatgcc agttgatgta tttgaaatgg aattgagcga caaagctgtt
     4741 cgccacgctg aattcctcgc ccaggatgac ggagttattc atctacttca cgtactaccc
     4801 gggtcagcca gcctgagcct gcaccgtttt gccgctgatg tgcgtcgttt tgaagagcat
     4861 ctgcaacatg aagcagaaga acgtttgcaa acgatggtca gccacttcac catcgatcct
     4921 tcccgcatta aacaacatgt ccgttttggt agcgtgcggg atgaagtcaa tgagttagca
     4981 gaagaactgg gggctgatgt tgtagttatt ggttctcgca acccatcgat ttcgacccat
     5041 ctgttaggtt ctaacgcctc gagcgtgatc cgccacgcca atctgcctgt actggtcgtt
     5101 cgctaacaga taaaagtatt tatgccgccc tgcctggaac atcccggcag ggctttttaa
     5161 tgccataaaa aagccgccag gtttgacccc ggcggctaag caattgcagg tgaatcttac
     5221 ttcttcttat gcagttttgg tgcgaatcag gtagtcaaaa gcactcagag aggctttggc
     5281 accttcgcca gtggcgatga tgatctgctt gtacggaacc gtcgtacagt cacccgctgc
     5341 gaacacgcct ttcacgttgg tttcgcattt cgcatcaatg ataatctcgc ccatgcggtt
     5401 acgttcgact gcgccttcga gccagttggt gttcggcagc agaccaatct gcacgaaaat
     5461 accggccagt tcgatgttgt gaatatcgcc gctgacacga tctcgatatt ccagaccaac
     5521 gactttgctg ccgtcgcctt tcacttccgt ggtttgcgca ttcagaataa tgtcgacgtt
     5581 tttcaggctg cgcagtttgt cctgcagaac ctggtcggct ttcatttctg gcgcaaattc
     5641 cagcagcgtt acgtgctcaa cgatacccgc caggtcaatt gccgcttcca cgccggagtt
     5701 accgccgccg ataaccgcta cgcgtttacc tttaaacagc gggccgtcgc agtgcgggca
     5761 gtaggtcacg cctttggtgc gatactgatc ttcgcccgga acgttcatgt tgcgccattt
     5821 tgcaccggtc gccacgataa tgctgcgtgc tttcagtacc gcgccagaag ctgtttcaat
     5881 ctgatgcaga ccaccttcaa ctgctgctgg gatcagtttg ctggcgctct ggctgtcgat
     5941 cacatcaacg tcgtattcat caacgtgaac tttcagtgcg cctgccagct tctgcccttc
     6001 agtcttcggt acagaaatgt agttttcgat atcaacggta tcgaggatct gaccaccaaa
     6061 acgttcgccc atcagaccgg tacggatgcc tttacgtgcg gagtaaattg ctgccgctgc
     6121 acccgccggg ccggaaccga cgattaatac gtcataagca tcacgcttgt tcagctcttc
     6181 tgccgcacgt ttttccgcgc ccgtatcaat tttggcaacg atttcagtca aagtcatgcg
     6241 gccctgacca aactctttcc cgttcacgaa cactgccgga acgcccatca cgttacgatc
     6301 agtgatctcg ttctggaagg tgccgccgtc aattgcagtg tgcttgatgc gcgggttcaa
     6361 tacgctcatc aggttcagcg cctgtaccac gtccgggcag ttatggcaag agagcgagta
     6421 ataggtttcg aattcaaaat caccgtcaat atggcgaatc tgctccagca gagactgcgc
     6481 ttctttcgac ggatgaccac cggtccacag taacgccagt accagcgagg tgaactcgtg
     6541 gcccagcggg gagcctgcaa aacgtggccc ctggttagaa cctgggttgg tgatcaggaa
     6601 agacggctta cgcaccggca agctgttatc ttctttaaag gtgactttgt ctgacagttc
     6661 tgcgatttca gccaacagtt ccttgatttc tgccgattta gcgctgtcat ccagcgtggc
     6721 aattaactca acaggcttgg tcaatttctc aaggtaagcc ttgagttgag ttttcatatt
     6781 tgtgtcgagc atgtttatct cctgggcttt aaacatcatc atgcaagcgg cctctgcggc
     6841 tacctggatg cagcttgcat catggtgcgg ggaagaaaat cgggtgccgc agcacccgat
     6901 taattacgcg attgcggaac ttagatttta ccaaccaggt ccagagacgg agccagagtt
     6961 gcttcacctt ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactgt
     7021 gctgctttga ttttacgcag caggtcagac gcgtcacggc caatgccttc agcggtaact
     7081 tcgattgcct ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct
     7141 tcatcttcac gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc
     7201 gcatatttga ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga
     7261 gtatcggtag atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca
     7321 gcaacgtcac ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag
     7381 acgctccagc ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat
     7441 gcctggtttt taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg
     7501 ttttcgatga gatgtaaggt aaccaatttt taccgattcg gctaatgcgt ttccattatc
     7561 aaatcgataa gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca
     7621 gcggcgataa aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt
     7681 tggtatcttc agcgccagtc tgcaggctgc gttgttgctc ttacaaatgc aacaacgaca
     7741 tggattacaa caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac
     7801 aatgataggt tatgcctttt actcgacttt tgcactgact gaaaaggaca aattaatgtt
     7861 aaaaaagata cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttcctgc
     7921 tccagtaaaa gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg
     7981 aggaatgaaa ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc
     8041 agatggtaag cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa
     8101 cacacttatc gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga
     8161 acaatcccac tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttcgc
     8221 cgatccgttc tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc
     8281 tggcaaagta cagttgagaa cattgttggt tggggttatc aaaccagaaa gtccggcgac
     8341 agccgcggca attcttgcct ccaaagatcc cgcaaaaacc tggcaagaat atgaagcctc
     8401 tggtggcaag cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt
     8461 aagcgacaac gagaaactca tggacgatct gggggcaaat gtcactccgg ctatctatta
     8521 catgagtaag gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa
     8581 tatcattatg ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt
     8641 tcaaccatcc taaaaaagca tgaattgctc gctctgccct aagtggctgt gctattttag
     8701 aaaaacttaa tcctgagtta agcaaaaaaa acataatcaa taaaaatata
//
LOCUS       DABSYT020000151         8709 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.151, whole genome shotgun sequence.
ACCESSION   DABSYT020000151 DABSYT020000000
VERSION     DABSYT020000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.151"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1058)
                     /locus_tag="ID725_RS22445"
                     /old_locus_tag="pgaptmp_004489"
     CDS             complement(<1..1058)
                     /locus_tag="ID725_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGV7852947.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLLRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRY"
     gene            complement(1068..2249)
                     /locus_tag="ID725_RS22450"
                     /old_locus_tag="pgaptmp_004490"
     CDS             complement(1068..2249)
                     /locus_tag="ID725_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGV7852948.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(2317..3342)
                     /locus_tag="ID725_RS22455"
                     /old_locus_tag="pgaptmp_004491"
     CDS             complement(2317..3342)
                     /locus_tag="ID725_RS22455"
                     /old_locus_tag="pgaptmp_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGV7852949.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(3773..3994)
                     /locus_tag="ID725_RS22460"
                     /old_locus_tag="pgaptmp_004492"
     CDS             complement(3773..3994)
                     /locus_tag="ID725_RS22460"
                     /old_locus_tag="pgaptmp_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852950.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(4247..7396)
                     /gene="acrF"
                     /locus_tag="ID725_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /pseudo
     CDS             complement(4247..7396)
                     /gene="acrF"
                     /locus_tag="ID725_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(7408..8565)
                     /gene="acrE"
                     /locus_tag="ID725_RS22470"
                     /old_locus_tag="pgaptmp_004494"
     CDS             complement(7408..8565)
                     /gene="acrE"
                     /locus_tag="ID725_RS22470"
                     /old_locus_tag="pgaptmp_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGV7852951.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2320 a         2249 c         2115 g         2025 t
ORIGIN      
        1 ctatagcgcg acatgctgaa acagaagatc cagtagatca gcgcggcgaa aacataccct
       61 tccgtcgaca tacccaacca ggcgggatca acggttgctt gctgaacgct actgaaaaga
      121 tcgaacaacc cgataatgat caccaggctg gtatctttga agagtgcgat gatggtattt
      181 accagcccag gaattaccag cttcaacgcc tgtggcagaa taaccagccc ctgagttttc
      241 cagtaaccca acgccagcga ctctgccgct tcatattgcc ctttaggcag cgcctgtaat
      301 ccacctcgca cgacttccgc aacatatgct gactgaaaca ggatcacgcc aaccagcgcg
      361 cggagcagtt tgtcgatact ggtgccttct gccataaaca aaggcagcat gaccgaagac
      421 ataaacagaa cggtaatcag cggcacgccg cgccagaatt cgataaaaat gaccgataag
      481 atgcgcacaa tcggcatatg ggagcggcga cctaacgcca gtaagatccc ccacggtagc
      541 gcccccgcaa tcccaactga tgcaataatt aaagttagcg tcagcccgcc ccattgccgg
      601 gtttcaaccc gctcaagagc aaaaaaaccg ccatacatca gccaccagac aatcagtggg
      661 taaatcactg cccagaccgc aatatagcga ccgcgatgcg ggagtatttt ccagaacatt
      721 ggtgcgattg acacaaggcc aatcagtaat gccaagttaa ttcgccagcg ttggtcgtgt
      781 gggtaaagtc catacataaa ctgaccaaat cgttcgtgga tgaagaccca acaggcaccg
      841 gcttttgtgc agtcggcacg cgttgagcca acccagttag cctgcaaaaa tgcccagttc
      901 agcaacggtg ggatcagttc ccacatcaac catatgcagc caatagtcag caggctattg
      961 ctccagctgg agaacagatt ttttcgcacc cacaccatcg cgcggcttga gttatggctc
     1021 gccgggcgcg agggatgaga cagcaatact tttgtcatca tgactcctta gcgttccacg
     1081 atcgcgatgc ggcggttata gatattcatc agcagcgaga tagtcaggct gataatcaga
     1141 tagaccgaca tggtcatggc tatcgtctcg atggcttgcc ccgtctggtt cagcacggtg
     1201 ccagcaaaca gcgaaaccat atcgggatag ccaatagcgg cggcaagaga ggagtttttg
     1261 acgatgttga gatactggct ggttaacggt ggaataatca cccgcagtgc ctggggaata
     1321 atgacctggc gtagcgtaac cgggttgggt aatcccagtg accgagccgc ttcatgttga
     1381 ccataaggca ctgcctggat ccccgcgcgg ataatctcgg cgataaatgc agaggtataa
     1441 accgaaagtg ccagcgttaa ggctgccagt tcaggaatta aaaccatccc gccgcggaag
     1501 ttaaagcctt gtagggctgg gacatcccag tgtagtgctg cgccaaaaag ccattgcgcc
     1561 agtaaaggca aaccgatgat caacactgcg gcgatgggcc aggtgcggcg cagctgtccg
     1621 gtctttatct ggtatgtttt attaaagcgg aatagcccaa cagaaaggac tatagccata
     1681 acaacagcca ggataaacgc aataaatccg tctcccagct gcggtgacgg aatataaagc
     1741 ccacgattgc tcaaaaaggc cagatcaaac gcgctcacag cctggcgtgg tccgggcaaa
     1801 ttgcgcaaca cggcaaagta ccagaagaag atttgcagca gcgggggaat attacggaag
     1861 atctcaatat aaattgtgga aagctttcgt agcagccagt tatccgaaag tctcgccaga
     1921 ccgataaaga agcccaaaac agaagcgaac acaatacaca atgcagaaac cagtagcgta
     1981 ttgagtaagc cgacaataaa aacgcgtccg taggtgtcgc cctgctggta atcgatcaaa
     2041 tgctggacaa taccgaagcc agcgccgcga tccagaaagg caaaacctga agtaatgcca
     2101 cgattattga gattcgttac agtgttgtga aataaccaac caacaatgcc gacaacagca
     2161 acaacggcaa ggatctggaa taaccaggcg cgaaccgtag ggttggcaaa agagagtgag
     2221 cctttaacgg ttgagcggcg atgagacata gaaacctcgg aaagcataac gctgaagaga
     2281 gcgccgccgt agcgacgctc gttacctcac accgacttaa cgcacgggcg gtgcgtactg
     2341 aataccgccg ttattccaga gattattttg cccacgttta attttcagcg ggctttctga
     2401 acctacgtta cgctcgaaaa tttccgagta gttacccacc tgtttgatga tgttataggc
     2461 ccatttatta tccaacttca gatccttgcc gtaatcgcct tctttaccca gcagatgtgc
     2521 catatcaggc gttgctggat tagccgcttt ttcatcgacg ttctgggaat tgatgcccat
     2581 ctcttcagca tttagcatgg cgaaaagcgt ccagcgtaca atcgagaacc attcatcatc
     2641 gccacgacga actaccggcc caagcggttc tttagagata acctccggta agacaatcca
     2701 ttcagccggg ttgcttaatt tgatgcgcag ggcatacagt tgggattgat ccgaagccag
     2761 cgtatcgcag cgaccggatt ccagtgcctt cgctgattcg tcagaacgat cgaaagtcac
     2821 tggtgtgtac ttcatattgt ttgccttgaa atagtcggcg acgttgagtt cggtatcagt
     2881 acccgcctga atacagacgg tagcgccatc cagttctttc gcgcttttta gccccgcttt
     2941 atcgtgcgtc aggaagccaa tgccgtcgta ataagtgacg ccagtaaatg ccattcccat
     3001 cccggcatcg cgagatgaag tccaggtcgt attacgggag agcaaatcca cttcccctga
     3061 ctgtaaagcc gtgaagcgtt cttttgctgt gagtggggta tatttcactt tcgtgtcgtc
     3121 accaaataca gcagcggcaa caccacgaca aacatcaaca tcaatacctg aaaacttacc
     3181 gtctgcatcg gcataagaga acccaggtaa tccatcactg atcccgcatt gcacaaaacc
     3241 ttttttctga acggcatcaa gcgtcgcgcc agcatgcgcc tgatttgcaa cggcaagcag
     3301 cacgctggcg gcagccagtg tggctatcat catctttttc ataatgcttc ctgtgaggcg
     3361 aaaattatcg ttataggacg tttgggaaac gtcttttgcc tgtctgtggc catcctctta
     3421 taagcaaaag tagtgccagc ttttgcgcac gatttcgcgt cgcaataagc tgagtgtaga
     3481 caggaaataa taaaaacagc gccccgcaat ggtgcgaaaa tgaaacatgc gccatatttt
     3541 ggcgcaaaac atttacgcgt gttttactaa gcagaataat gattgttagc gtgagtatat
     3601 ttctggtggg aagtgcgaaa gttaaattga tgcgttattt cgaaagcagc gaaataacac
     3661 gatataacat gctaaatcag cagagaatta gccagcagaa tatagcaata ttttatctta
     3721 tgtaaagata ctgtatcgtt aaacagagcg cgaaactccg cgccctgttc agttatttcg
     3781 acagagcgat gatccccatc gtcaggccag caacagccgc tgccccgcca gcaatagccc
     3841 cagccgtttc ccagttatcc tgagtcgtgc ctttcataca ggtattggtg tgaaccagac
     3901 gaccctgatc gtcataaacc ggaacgcagg gcgaatcgtg agcacagccc gccagcaatg
     3961 cggtgagcaa tgccacagga attaatcttt tcatggtgtt acctcagtaa taaacgctaa
     4021 cgcttaataa gttaactctc ttattaacga gctgaatgcc attttatcac cggaggttat
     4081 aaatcttgcg gaatgaataa tgacaaatta tgtgaattgt aaaaatagtg aagggagcgg
     4141 gagctataga aagcttttaa agagtgagca aaagaatatc ttattaattt ttatgggttt
     4201 cactggaaaa cataaaggca cccgaaagcg cctttatgtt tcagatttat cctttaaagc
     4261 aacggcggat caccacaaag aacaccggta cgaagaagat tgccagcaac gttgcagaga
     4321 ccattcctcc cattaccccg atacccactg cgttctgcgc gccactgccg gcaccgttac
     4381 tgatagccag cggtaatacg ccgagaataa aggcgagaga ggtcatcagg ataggacgca
     4441 gacgcatacg tactgccatt agtgtcgctt caacaacacc tttaccctct ttctccatga
     4501 gatctttagc gaactcaaca atcaaaatag cgtttttggc cgacaagcca attgtcgtta
     4561 gcaagcctac cataaagtag acgtcatttt tttgattaaa gagtgtcgcc gccagcagca
     4621 cgccgacaat ccctaacggc acgaccaaca taaccgagac aggaattgac cagctttcat
     4681 agagtgcagc aaggcacagg aaaacaacca caaaggaaat tgctaccaga gcgggagcct
     4741 ggtttcccga taagcgttcc tgatacgaca tacccgtcca gtcataacca atgcccgcag
     4801 gtaattttga cgctaggttt tccatcaacg ccatggcatc gccggaactg gttcctggcg
     4861 cggcttcccc ctgaatctcc attgacggca gaccgttgta gcgttccagt cgcggagagc
     4921 catacaccca atgtgaagtg gtaaaggcag agaatggcac catttcgccg ttggtgctgc
     4981 ggacataaag tttatcgaca tcttctggca gcatacggaa tttggcatcc gcctgaacat
     5041 acaccttttt cacgcgacca cggtcgatga agtcgttaac gtaagtccca cccaacgccg
     5101 ttgaaatggt ctgattgatg tcagaaagtg aaacacctaa tgcctgcgcc ttttcctggt
     5161 caacttccag tttaaactgc gcggtgtctt ccaggccatt gggacgcacg ctgactaagc
     5221 tggcaggatg ttgcgccgcc ataccaagca actggttacg ggcctgggtt agggcatcgt
     5281 gacccagccc agccttatca attaactcaa agtcgaaacc cgttgccgtg cccagttcaa
     5341 caatggctgg catattgaat ggaatgacaa aaccgtcgcg gatcttgccc aactccattt
     5401 tggcacgatg gattaccgct tccgcactgt tttcatcacc attacgctct tcccacggtt
     5461 tcagactgac gaaggccata cctgtagatt caattggtca acgcaacagt tatgtgaaaa
     5521 catggggttg cggaggtgcc tggccgctga agctaaagcc gttaaccgta aagatacttt
     5581 caacgtttgc tttctcgttc ttcagatagt aatccgtcac ttgatccaac actttttgcg
     5641 tccgctcttg cgtcgcgcca gcgggtaact gaatcatggt cagaaagaca ccctgatcct
     5701 cttcaggtaa gaaggaagac ggaagacgta aaaacaacac caccattcct gcaacaatca
     5761 gcgcatagat cagtaaatat cgtcctgtgg aaccgaggat tttgccgacg ctgttggtgt
     5821 agtggttaac gctatgatcg aaggtggtat taaaccagcc gaagaaacca cccttatttt
     5881 cgtgatgctc agctgaggtg ggtttgagca gtgttgcaca taacgcaggg gtaagaatca
     5941 atgccaccag aacagaaagc gccattgccg aaacgatggt gatagagaac tgacgataaa
     6001 ttgccccagt agaaccgccg aagaatgcca tcggaataaa taccgctgac agcaccatcg
     6061 cgatacccac cagtgcgccc tgtatttgcg acattgattt ttccgtcgct tcttttggcg
     6121 ggagcttatc ctccatcatc acgcgctcga cgttctccac caccactatc gcatcatcga
     6181 cgagcagccc gatggcaagc accatcccga acatcgttag tgtgttgatg gagtaaccaa
     6241 aagcggcgag gatggcaaac gtccctaaca acaccacggg taccgcaatg gtggggatca
     6301 gcgttgctcg catattctgc aagaacagat acatcaccag gaacaccagc ataatagctt
     6361 cgaacagtgt ttttaccact tcgtgaatag aaagctggac gaatggcgtg gtgtcataag
     6421 ggtagagaac cttcattccc tgcgggaaga atggctgtaa ttccgccagt tttgccttaa
     6481 tggctttcgc ggtatcgaga gcattcgcgc cggttgccag cttaataccc aggcccgccg
     6541 ccggttttcc gttgatgcga gcgataacgt tatagttttc accgccaagt tcaacccgtg
     6601 cgacatcttt cagacgcacc accgagccgt cactgtttac gcgcagggtc actttgccga
     6661 attcttccgg attttttaac cgcgtctgag caataatcga ggcattcaac tgttgccctg
     6721 gtaacgctgg cgttccgccc aactgtccgg cagcgatctg agcgttctgt accttcaact
     6781 ggttaatcac atcaaccggt gtcagtttat atttgtttag cagatcggcg tccagccaga
     6841 tacgcatcgc atactgtgcg ccgaaaagct gtacgtcacc gacgctattc agacggcgaa
     6901 gcgtatcttt aacgttagag gccacatagt ccgagatatc gtcctgtgtg gtgtctgggt
     6961 tatcagagac aaagcctgcc accatcaaat agctgctact ggacttttca acactgatcc
     7021 cctgctgctg aacctcctgc ggcagcaacg gcgtggcgag ctggagtttg ttctgcacct
     7081 gcacttgcgc gatatcagga tcggtcccgg actggaaggt aagggtaatt gtcacgctac
     7141 cggcggaatc gctggtggag gacatataca tcaggttatc gataccgttc atattctgtt
     7201 cgataacctg cgtcaccgta tcctgcacgg tctgcgcatc agcgcccgga tagtttgctg
     7261 aaacagaaac cgcaggcggt gcaatggttg gatactgagc gacgggcaat tgtaggatcg
     7321 ccagtgcgcc cgccatcatc agaataatgg ccagcaccca tgcaaatatc ggtcgtcgaa
     7381 taaaaaagtt tgccatgtca gattacctta cttcgatgca gtatctgcgg gggtatcggt
     7441 agtggcttta acctgctctc ccggacgcgc tttttgcagg ccgctgacaa tgacttgatc
     7501 gccagatttc agaccttcgc taatcaacca tttatcgcca atcgcctgac tggcaacgac
     7561 agggcgcact tccacctgac ttttatcgtt aacaatcagc acggttgcat caccacgcgg
     7621 tgtgcggcta acgccttgtt gcgggatgag aatggcgtca ggttggacgc cttcatcaat
     7681 ccgtgcacgc acaaacatac ccggcaaaag cgtatgttgc gggttaggga agacagcacg
     7741 tagggttatg gagccggtgc tttcatcaac ggtcacatcg gagaattgca gcgtaccttt
     7801 caggggatag gtttgaccgt tttccatgac caactctacg ttgctggtgg cgttttcctt
     7861 atgcaaattt ccttgctcta cggactgctt cagcctcata aaatcgttgc tggattgggt
     7921 cacatcaaca tagataggat cgagctgctg gacagttgcc agttcagtcg tttgcccatt
     7981 agtgacaaga gcgccttcgg tcacagtcga tttgccgata cgtccgctaa ttggcgcagt
     8041 cactttggta taagcaagat tgatgcgagc gctttcgact gtggcttttg cggcaatcac
     8101 ggcggcatcg gcctggcgag catcagcaat ggcctggtcg tactcctgct ggctgatgta
     8161 tttcgtaccc acgagcggaa cgtaacgttt taccgtcaaa tgcgcgatgg cggcggcggc
     8221 ttcacttttc gccagttcgc ctttcgcgct gtcataattt gcctgatagg tcgcgggatc
     8281 gatctggtac agggactggc ctgcttgcac atcgctgcct tcagtgaaat tgcgattcag
     8341 tacgatcccg ctaacctgtg ggcgaacttc ggctatacga taagcattgg tgcggcctgg
     8401 taattcagtc ttaacttcta acggggccgt ttttacaata tgaacggtaa cctgcggttc
     8461 accgacgtga gctttctctt ctcccttatc gttacaaccg gcgattaaag ccgcggagat
     8521 cagaataaag gagggcagga ggaaaaacct ggcatgtttc gtcattacta ttcctcaaaa
     8581 aaccaaaagc gcgttattta cccaaaaagg caacgcgtta actcgcagaa agaaaaatac
     8641 agttcgctat cctacaaatt atcattcgtc gatgtaagga atagttatga atacaggcgt
     8701 ctcaaggca
//
LOCUS       DABSYT020000152         8630 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.152, whole genome shotgun sequence.
ACCESSION   DABSYT020000152 DABSYT020000000
VERSION     DABSYT020000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.152"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..356)
                     /locus_tag="ID725_RS22475"
                     /old_locus_tag="pgaptmp_004495"
     CDS             complement(<1..356)
                     /locus_tag="ID725_RS22475"
                     /old_locus_tag="pgaptmp_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGV7852952.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAG"
     gene            complement(353..1141)
                     /locus_tag="ID725_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /pseudo
     CDS             complement(353..1141)
                     /locus_tag="ID725_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            1281..1517
                     /locus_tag="ID725_RS22485"
                     /old_locus_tag="pgaptmp_004497"
     CDS             1281..1517
                     /locus_tag="ID725_RS22485"
                     /old_locus_tag="pgaptmp_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGV7852953.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            1507..2649
                     /locus_tag="ID725_RS22490"
                     /old_locus_tag="pgaptmp_004498"
     CDS             1507..2649
                     /locus_tag="ID725_RS22490"
                     /old_locus_tag="pgaptmp_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGV7852954.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(2763..4013)
                     /gene="icd"
                     /locus_tag="ID725_RS22495"
                     /old_locus_tag="pgaptmp_004499"
     CDS             complement(2763..4013)
                     /gene="icd"
                     /locus_tag="ID725_RS22495"
                     /old_locus_tag="pgaptmp_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGV7852955.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            4185..4838
                     /gene="rluE"
                     /locus_tag="ID725_RS22500"
                     /old_locus_tag="pgaptmp_004500"
     CDS             4185..4838
                     /gene="rluE"
                     /locus_tag="ID725_RS22500"
                     /old_locus_tag="pgaptmp_004500"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGV7852956.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            4848..5309
                     /gene="nudJ"
                     /locus_tag="ID725_RS22505"
                     /old_locus_tag="pgaptmp_004501"
     CDS             4848..5309
                     /gene="nudJ"
                     /locus_tag="ID725_RS22505"
                     /old_locus_tag="pgaptmp_004501"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGV7852957.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            5363..6469
                     /gene="mnmA"
                     /locus_tag="ID725_RS22510"
                     /old_locus_tag="pgaptmp_004502"
                     /gene_synonym="trmU"
     CDS             5363..6469
                     /gene="mnmA"
                     /locus_tag="ID725_RS22510"
                     /old_locus_tag="pgaptmp_004502"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGV7852958.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            6505..7146
                     /gene="hflD"
                     /locus_tag="ID725_RS22515"
                     /old_locus_tag="pgaptmp_004503"
     CDS             6505..7146
                     /gene="hflD"
                     /locus_tag="ID725_RS22515"
                     /old_locus_tag="pgaptmp_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGV7852959.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            7150..8520
                     /gene="purB"
                     /locus_tag="ID725_RS22520"
                     /old_locus_tag="pgaptmp_004504"
     CDS             7150..8520
                     /gene="purB"
                     /locus_tag="ID725_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGV7852960.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
BASE COUNT         2183 a         2199 c         2110 g         2138 t
ORIGIN      
        1 ccggcgataa cggatgcttc ttcccggcac catgcaacaa actgcccggg tgatggcaga
       61 aatggtcgat tctgccgacg ggctacgcgc attcctgcgt taacctgttc cattgtggtg
      121 atcccgtttt cccggaaagc cagaacccac tggcggcgga tttcgttcag ttcgttctgg
      181 tcacggttag ccagactcgc cgggaaagtt gccagtaact ggctgaacac accgttgatg
      241 atctgcgcta cctgctgtac ctgcggcttt tcgtcgtact gttccggcat gttgttggcg
      301 atccgacgca tctgctcacg gtcaaagtta accatctgtg cggcgatgtt tttcataaat
      361 ccaccccgta aatccagtca gtgtttgtca ggtcgagttt tggtttgctg gctatcatgc
      421 ctgcctgttg cttgttacgg ttgatttcga gctgggtcca cttgtcgcgg agtttggccg
      481 gacttagcac gttaccggac cagaagttgt cctggcatgc ccagcggaac agcacgcaca
      541 tgtcgcggtg gttacgtccg tcacgttcac gcatcaggcg gatatcgtta gcccaccctg
      601 caaaattcgg ttttctggct gatggcgcga tggtcttcac catgtcaaac atccactctg
      661 cggcggtcag gtcttctgct gtcccccact tgctgccgct ctgaattgca gcatccggtt
      721 tcaccacagg aagatcgttt tctggttggt cagaggattc gccagaattc tcggacgaaa
      781 aaggttttat attgtctttt gttagtttgt cttttgtgtt tacctgattc gggtaaacgc
      841 ctttacctga tttgggtaaa ctttttttac ctgattcagg taaatttacc tctttcaggt
      901 aaactttatt tttcttacct gattcgggta atgttgacca ttcactgacc acattattaa
      961 tgccggtatt ccgcccgctc tgaataaaaa tcccacgctt taccagaaca ctttttgcag
     1021 cagaacactt gtgcggcaat atcccggtta attcggaaag ttgctcgttg ctaacccaat
     1081 ccagtttttt attaaagcca tatgttttgc gcatgacagc cagaaagacc agaagctggt
     1141 gaaaaattga taaattcaat actgcatttc tcagcattaa atttatctct atgaccagtc
     1201 aagagatgta cctgccatga gcttaatatc atgtcagata tatcggtcac aaactccctc
     1261 agcagctaag aggaggacaa atgtctcgac taatcacttt acgggactgg gctaaagaag
     1321 aatttgggga cttagcacca agtgagcgag ttctgaaaaa atacgcgcaa gggaaaatga
     1381 tggccccacc cgctataaaa gttggtcgct actggatgat tgaccgaaat tcccgttttg
     1441 taggaacgct tgcagaaccg caactcccaa taaacgcaaa cccaaaactc caacggataa
     1501 tcgctgatgg ctgctagccc ccgatctcac aaaatctcta tacccaattt atattgcaaa
     1561 ttagataagc gaaccggaaa ggtatattgg caatacaaac atccactatc cggtcgtttt
     1621 catagcttag gaactgatga gaatgaagca aaacaagttg ctactgaagc aaataccatt
     1681 attgctgaac aacgtacccg acaaatatta agcgtcaatg agcgtctgga aagaatgaaa
     1741 ggcaggcgct cagacattac ggtgacagaa tggcttgata aatataattc tatccaggag
     1801 gacaggctgc aacataatga actaagaccc aactcctatc ggcaaaaagg caaacccatc
     1861 cgtcttttcc gtgagcattg tggaatgcaa cacctcaagg atattaccgc acttgatatt
     1921 gccgaaataa ttgatgctgt aaaggctgaa ggtcataaca ggatggcgca agtcgtgaga
     1981 atggtgttga tcgacgtctt caaagaagca caacacgcag gacatgttcc gccaggattt
     2041 aacccagcgc aggcaacaaa acaaccgcga aatcgagtaa accgtcaaag attatcactg
     2101 cccgaatggc aggcaatatt tgacagcgta agcagacggc agccctattt aaaatgcggg
     2161 atgctacttg ctcttgtcac tggacaacgt ttaagcgata tctgcaattt gaaattctct
     2221 gatatctggg acgacatgtt gcacattact caggaaaaaa ccggttcaaa acttgctatt
     2281 ccgcttaacc tgaaatgcga tgctctgaat attacccttc gtgaagttat atctcagtgc
     2341 agggatgctg ttgttagtaa atatctggtc cattaccgtc acactacctc tcaagcaaac
     2401 agaggagacc aggtgtctgc aaatactctt acaacggctt ttaaaaaggc cagggaaaaa
     2461 tgtggcataa aatgggagca aggaactgcg cccacatttc atgagcagcg atctctgtca
     2521 gaacggttat atcgggaaca gggtctggat acgcaaaagt tgttaggtca taaatccaga
     2581 aaaatgaccg accgatacaa tgatgatcgt ggtaaagact ggattatcgt agatatcaaa
     2641 acagcataga aaatagccag ttttggggaa gggttttggg gaaagttttg gggaagattt
     2701 taaatcatca taaaacaacg ggcgtgttat acgcccgttt caatatttaa cacatgtagt
     2761 gattacatat tcttgatgat cgcgtcacca aactctgaac atttcagcag tttagcgcct
     2821 tccatcagac gttcgaagtc ataggttacg gtcttggcat tgattgcgcc ttccatacct
     2881 ttaacaatca ggtcagccgc ttcagtccaa cccatatggc gtaacatcat ctcagcagag
     2941 agaataatag aacctgggtt aactttgtcc tgaccggcat atttcggcgc agtaccgtgg
     3001 gtggcttcaa acagggcgca ttcgtcaccg atgtttgcgc caggggcgat accgataccg
     3061 ccaacctgcg ctgccagggc gtcagaaatg tagtcaccgt tcaggttcat acaggcgata
     3121 acatcatatt cagccggacg cagcaggatc tgttgcagga atgcatcagc aatcacgtct
     3181 ttaatgacga tctctttgcc ggtgttcggg tttttaactt tcagccacgg gccgccgtcg
     3241 atcagttcac cgccaaactc ttcacgcgcc agctcgtagc cccagtcttt aaacgcgcct
     3301 tcggtgaact tcatgatgtt gcctttgtgc accagagtca cagagtcacg atcgttagca
     3361 attgcgtatt cgatcgctgc acgaaccaga cgtttggtgc cttcttcaga acacggctta
     3421 ataccgatac cgcaatgttc cgggaagcga attttcttca cgcccatctc ttcacgcagg
     3481 aatttaatca ctttctcggc gtcggcagag tcagctttcc attcgatacc cgcataaatg
     3541 tcttccgagt tttcacggaa gataaccata tcggtcagtt cagggtgttt aaccgggctt
     3601 ggagtgccct gatagtaacg taccggacgc aggcagatgt agagatccag ttcctggcgc
     3661 agggcaacgt tcagagagcg aataccgcca ccaaccggag tggtcagcgg gcctttaatg
     3721 gcaacgcgat attcacgaat cagatcaagg gtttcagcag gcagccagac gtcctgacca
     3781 taaacctgtg tggatttttc accggtgtaa atttccatcc aggagatttt acgctcgcct
     3841 ttataggctt tctcgactgc agcgtcgacc actttcagca tggctggggt tacatctaca
     3901 ccgattccat caccttcaat gtaagggata atcggatttt caggaacgtt gagtttgccg
     3961 ttttgcaggg tgatcttctt gccttgtgcc ggaacaacta ctttactttc cattcacctc
     4021 tccttcgagc gctactggtt tgctcgtctg ccaccacgtt gcatatgcgt ttgcgtcctg
     4081 cgatacggat gctttagagc aattttttgt taatgatttg taattggctt gtcaatacta
     4141 ccctattgtt tgccgtcatg aaagatgcgc gttattaggc tataatgcgg caattcataa
     4201 tctctgaaaa taccatgcaa aaaacttctt ttagaaatca ccaggttaag cgattcagct
     4261 cacaacgttc taccaggcgt aaacctgaaa accagcccac gcgtgtgatc ctgttcaata
     4321 aaccctacga tgttcttccg cagttcaccg atgaagccgg acgcaaaaca ttaaaagaat
     4381 tcatcccggt tcagggcgtt tatgcagcag gtcgtcttga ccgcgatagc gaagggttac
     4441 tggtgctgac caataacggc gcgttgcagg cgcgtttaac ccagccgggc aaacgcaccg
     4501 gaaaaatcta ttatgtgcag gtggaaggta ttcccacaca agacgcactt gaagccttgc
     4561 gcaatggcgt aaccttaaat gatggcccta ccctgcccgc aggcgcggag ctggttgacg
     4621 aacccgcgtg gttatggccg cggaatccac caattcgtga acgcaaaagt attcccacca
     4681 gctggctgaa gatcacctta tatgaaggac gtaatcgcca ggtgcgccgc atgaccgccc
     4741 atgttggttt ccccacgctg cgactgattc gctatgcgat gggtgattac tctttggata
     4801 accttgccaa tggcgaatgg cgagaagcaa cagaataagg atgcacaatg tttaaaccgc
     4861 acgttaccgt tgcttgcgta gtgcacgcag aggggaaatt tttagtcgtt gaagagacga
     4921 ttaatggtaa agccttatgg aaccaacctg ccgggcatct ggaagccgat gaaaccttag
     4981 tggaagcagc cgcccgtgag ctgtgggaag aaaccggcat cagcgcgcag ccgcaacact
     5041 ttattcgtat gcatcagtgg attgcgccag ataaaacgcc gtttttgcgt ttcctctttg
     5101 ccattgagct tgagcaaata tgcccgaccc agcctcatga cagcgatatc gactgctgtc
     5161 gttgggtcag cgccgaagaa attttacagg cgtcaaatct tcgttcgccg ctggtggcgg
     5221 aaagtatccg ttgttatcaa agcgggcaac gctatccgct ggagatgatt ggcgatttta
     5281 actggccttt tacaaagggt gtcatctaaa aggctgcgtg atagaatacg ccgccttgaa
     5341 gttcaatgtc gtgagtgatc caatgtctga aaccgcaaaa aaagtaatcg tcggcatgtc
     5401 cggcggtgtc gattcctccg tttctgcctg gctgttgcaa caacagggat atcaggtcga
     5461 aggcctgttt atgaagaact gggaagaaga cgacggtgag gaatattgca cagcggcagc
     5521 ggatctggct gatgcccagg ctgtctgcga caagctcggc attgaactgc acaccgttaa
     5581 ctttgctgcc gagtactggg acaacgtctt cgaactgttc cttgccgaat ataaagccgg
     5641 tcgcacgccg aatccggata ttctgtgcaa caaagagatc aaatttaaag ccttcctcga
     5701 atttgccgcc gaagatttag gtgccgatta tatcgctacc ggtcattacg ttcgtcgggc
     5761 agatgtcgat ggcaagagcc gcctgctgcg tggtctggac agcaataaag accagagcta
     5821 cttcctttat acgctcagcc atgagcagat tgcgcaaagc ctgttcccgg tcggcgaact
     5881 ggaaaaaccg caggtgcgta agattgctga agatcttggt ctggtcaccg cgaagaaaaa
     5941 agactctacc ggcatttgct tcattggcga acgtaaattc cgcgagttcc ttggccgtta
     6001 tctcccggcg caaccgggca aaatcattac agtcgatggc gatgaaattg gtgagcatca
     6061 ggggctgatg tatcacaccc tcggtcagcg caaaggtctg ggtatcggtg gcaccaaaga
     6121 aggcaccgaa gaaccgtggt atgtggtcga taaagacgtc gaaaacaaca ttctgattgt
     6181 tgcgcagggc catgaacacc cgcgtctgat gtctgtcggt ctgattgccc agcagctgca
     6241 ctgggtcgat cgtgaaccat tcaccggtac tatgcgttgc acggtaaaaa cccgctatcg
     6301 ccagaccgat atcccctgca ccgtcaaggc gctggacgat gatcgcattg aagtgatttt
     6361 cgatgagccc gttgccgccg tgacgccggg ccagtctgcc gtcttctata acggtgaagt
     6421 gtgcctcggt ggcggtatta ttgagcagcg tctgccgctg ccgatctgat tattatcttt
     6481 acttaaaaca aggaagcagt gaacgtggca aagaattact atgacatcac cctcgccctg
     6541 gccggtattt gtcagtcggc acgcctggtg caacaactcg ctcaccaggg gcattgtgat
     6601 gccgatgcgc tacacgtctc actcaacagt attattgata tgaaccccag ctcgacgctg
     6661 gcggtttttg gcggtagcga agccaacctg cgcgtcgggc tggaaaccct gctcggcgtg
     6721 ctcaatgcca gcagtcgcca gggcttaaac gccgaattaa cccgctacac actcagcctg
     6781 atggtgctta agcgcaaact ctcctcagcg aaaggtgcgc tcgacactct gggcaaccgg
     6841 atcaacggcc tgcaacgcca gctcgaacac ttcgatttac agtccgaaac gctgatgagc
     6901 gcgatggctg ctatctatgt tgatgtgatc agcccgcttg gcccgcgcat tcaggtcacc
     6961 ggttcccctg ctgtactgca aagcccacaa gtgcaggcga aagttcgcgc aaccctgctg
     7021 gcaggcattc gcgccgccgt gctctggcac caggtcggcg gcggacgtct gcaactgatg
     7081 ttttctcgta atcgcctgac cactcaggca aaacaaattc ttgctcattt aaccccggag
     7141 ttgtgatcta tggaattatc ctcactgacc gccgtttccc ctgtcgatgg acgctacggc
     7201 gataaagtca gcgcgctgcg cgggattttc agcgaatatg gtttgctgaa attccgtgta
     7261 caagttgaag tacgttggct gcaaaaactg gccgcgcacg cagcgatcaa ggaagttcct
     7321 gcttttgctg ccgacgcaat cggttacctt gatgcaattg tcgccaattt cagcgaagaa
     7381 gatgccgcac gcatcaaaac catcgagcgt actactaacc acgacgttaa agcggttgag
     7441 tatttcctga aagaaaaagt ggcggagatc ccggaactgc acgcggtttc tgaattcatc
     7501 cactttgcct gtacttcgga agatatcaat aacctctccc acgcattaat gctgaaaacc
     7561 gcgcgtgatg aagtgatcct gccgtactgg cgtcaactaa ttgatggcat taaagatctc
     7621 gccgttcagt atcgcgatat cccgctgctg tcccgtaccc acggtcagcc agccacgccg
     7681 tcaaccatcg gtaaagagat ggcaaacgtc gcctaccgta tggagcgcca gtaccgccag
     7741 cttaaccagg tggagatcct cggcaaaatc aacggcgcgg tcggtaacta taacgcccac
     7801 atcgccgctt acccggaagt tgactggcat cagttcagcg aagagttcgt cacctcgctg
     7861 ggtattcaat ggaacccgta cactacccag attgaaccgc acgactacat tgccgaactg
     7921 tttgattgcg ttgcgcgctt caacaccatt ctgatcgact ttgaccgtga cgtctggggt
     7981 tatatcgccc ttaaccactt caaacagaaa accattgctg gtgagattgg ttcttccacc
     8041 atgccgcata aagttaaccc gatcgacttc gaaaactccg aagggaatct gggcctttcc
     8101 aacgcggtat tgcagcatct ggcaagcaaa ctgccggttt cccgctggca gcgtgacctg
     8161 accgactcca ccgtgctgcg taacctcggc gtgggtatcg gttatgcgct gattgcgtat
     8221 caatccaccc tgaaaggcgt gagcaaactg gaagtgaacc gtgaccatct gctggatgag
     8281 ctggatcaca actgggaagt gctggcagaa ccaatccaga cagttatgcg tcgctatggc
     8341 atcgaaaaac cgtacgagaa gctgaaagag ctgactcgcg gtaagcgcgt tgacgccgaa
     8401 ggcatgaagc agtttatcga cggtctggcg ttgccagaag aagagaaagc ccgcctgaaa
     8461 gcgatgacgc cggctaacta cattggtcgc gccatcacca tggttgatga gctgaaataa
     8521 actcgtacca gtgccggatg gcgatgctgt ccggcctgct tattaagatt atccgctttt
     8581 tattttttca ctttacctcc cctcccccgc tggtttattt aatgtttacc
//
LOCUS       DABSYT020000153         8468 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.153, whole genome shotgun sequence.
ACCESSION   DABSYT020000153 DABSYT020000000
VERSION     DABSYT020000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.153"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(23..1399)
                     /locus_tag="ID725_RS22525"
                     /old_locus_tag="pgaptmp_004505"
     CDS             complement(23..1399)
                     /locus_tag="ID725_RS22525"
                     /old_locus_tag="pgaptmp_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGV7852961.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            complement(1396..2217)
                     /locus_tag="ID725_RS22530"
                     /old_locus_tag="pgaptmp_004506"
     CDS             complement(1396..2217)
                     /locus_tag="ID725_RS22530"
                     /old_locus_tag="pgaptmp_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGV7852962.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEITDSLLMADLT
                     VRQLKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAKSANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GDGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            complement(2204..2365)
                     /locus_tag="ID725_RS22535"
                     /old_locus_tag="pgaptmp_004507"
     CDS             complement(2204..2365)
                     /locus_tag="ID725_RS22535"
                     /old_locus_tag="pgaptmp_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852963.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(2398..2694)
                     /locus_tag="ID725_RS22540"
                     /old_locus_tag="pgaptmp_004508"
     CDS             complement(2398..2694)
                     /locus_tag="ID725_RS22540"
                     /old_locus_tag="pgaptmp_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGV7852964.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            complement(2836..3051)
                     /locus_tag="ID725_RS22545"
                     /old_locus_tag="pgaptmp_004509"
     CDS             complement(2836..3051)
                     /locus_tag="ID725_RS22545"
                     /old_locus_tag="pgaptmp_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7852965.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            3127..3822
                     /locus_tag="ID725_RS22550"
                     /old_locus_tag="pgaptmp_004510"
     CDS             3127..3822
                     /locus_tag="ID725_RS22550"
                     /old_locus_tag="pgaptmp_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGV7852966.1"
                     /translation="MDGSSTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLSDIGVVFKYLGIDNISFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERLVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     gene            4324..4845
                     /locus_tag="ID725_RS22555"
                     /old_locus_tag="pgaptmp_004511"
     CDS             4324..4845
                     /locus_tag="ID725_RS22555"
                     /old_locus_tag="pgaptmp_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852967.1"
                     /translation="MAKSNVSVQAFKDFLEELMSLNIMKEATARNLKNSSARLLTVVQ
                     EEEMGDVTQLDVNELAERYINATEPKPSDSSITAYKSRMESAIKKFVAFQSGEEIPYT
                     PIDKESSEEKDLTGEPTKVEGKANALHTYDLPVVLRPESGVTVTIKGIPNDITNEEAE
                     RISSILKVYVRPQ"
     gene            5414..5596
                     /locus_tag="ID725_RS22560"
                     /old_locus_tag="pgaptmp_004512"
     CDS             5414..5596
                     /locus_tag="ID725_RS22560"
                     /old_locus_tag="pgaptmp_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852968.1"
                     /translation="MEQTGRLFKQRRLSTTWLKSQITQPHKLWDAMPKQPSQEELRDC
                     IAKVYSGGIYVQKNRI"
     gene            5574..5846
                     /locus_tag="ID725_RS22565"
                     /old_locus_tag="pgaptmp_004513"
     CDS             5574..5846
                     /locus_tag="ID725_RS22565"
                     /old_locus_tag="pgaptmp_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGV7852969.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVALYAAGYRKSKQLTAR"
     gene            5905..6156
                     /locus_tag="ID725_RS22570"
                     /old_locus_tag="pgaptmp_004514"
     CDS             5905..6156
                     /locus_tag="ID725_RS22570"
                     /old_locus_tag="pgaptmp_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852970.1"
                     /translation="MEFHESAICDFRANANSVKPQPIAVLFKTMGAWAVLCFAADDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
     gene            6339..6707
                     /locus_tag="ID725_RS22575"
                     /old_locus_tag="pgaptmp_004515"
     CDS             6339..6707
                     /locus_tag="ID725_RS22575"
                     /old_locus_tag="pgaptmp_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HGV7852971.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSLLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            6780..6944
                     /locus_tag="ID725_RS22580"
                     /old_locus_tag="pgaptmp_004516"
     CDS             6780..6944
                     /locus_tag="ID725_RS22580"
                     /old_locus_tag="pgaptmp_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGV7852972.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            6913..7056
                     /gene="kil"
                     /locus_tag="ID725_RS22585"
                     /old_locus_tag="pgaptmp_004517"
     CDS             6913..7056
                     /gene="kil"
                     /locus_tag="ID725_RS22585"
                     /old_locus_tag="pgaptmp_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGV7852973.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            7131..7427
                     /locus_tag="ID725_RS22590"
                     /old_locus_tag="pgaptmp_004518"
     CDS             7131..7427
                     /locus_tag="ID725_RS22590"
                     /old_locus_tag="pgaptmp_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGV7852974.1"
                     /translation="MNAYYIQDRLEAQSWTRHYQQIAREEKEAELADDMGKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            7433..8218
                     /gene="bet"
                     /locus_tag="ID725_RS22595"
                     /old_locus_tag="pgaptmp_004519"
     CDS             7433..8218
                     /gene="bet"
                     /locus_tag="ID725_RS22595"
                     /old_locus_tag="pgaptmp_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGV7852975.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYNKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKVLGFLKQKASEQKVAA"
     gene            8215..>8468
                     /locus_tag="ID725_RS22600"
                     /old_locus_tag="pgaptmp_004520"
     CDS             8215..>8468
                     /locus_tag="ID725_RS22600"
                     /old_locus_tag="pgaptmp_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HGV7852976.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQY"
BASE COUNT         2316 a         2024 c         1954 g         2174 t
ORIGIN      
        1 attaactcag tgatgtagat ggtcatacgt cagccccttg tgcatatcgt ctgccacgcg
       61 cagcaggtgc atttgatgct gtgcaaatct gtctggcttc atcctggtca catgcaacaa
      121 agtgtccgtt acagaaccgc tggtaaactg taccaagcga gccaaaacgg tttttcgtca
      181 cgatgatttc agcaaatggc gcggcgctac tgttctcatc atataccgct tcccgataga
      241 gcatgatgat tgagtctgcg tcctgttcaa tgcttcctga atcacgcaaa tctgcgtttg
      301 tcgggcgttt gtttggtcgc ttctcaacat cgcgcgaaag ctgactcagg gagataaccg
      361 gtgttttcag gtctttcgcc atcgccttca ggcttccgga gatgtgagca attgcgaggt
      421 cgttgcggtc tgctttcggc ttctcaatca ggccaagata atccgccatg atgagtgaca
      481 ggtttggatt ttcctgctta tgccgttctg cgattgagcg tatttcttcg accgataacc
      541 gcgaagcatc gactacccat acatccaaat ctgcaagctg actcatgccg ttagcaacac
      601 gtgcccagcc ctcgtcatcc atcgatgcag gatttcgcag tacgctaacc gacatcctcc
      661 cggcgttggc aatgcttcgc tctgcaatct gcaatgcgct catttccatt gagaaaatca
      721 ataccccgcg ccggacgtca gaaccaggaa taacgcggct tgccacacct tcggcaatct
      781 tcagcgccag ttcggttttc cccataccag gacgagcggc gataatcacc aggtcttctg
      841 cgttcatccc tccagtgata gcgtcaagct cttcgattcc ggtcttcagg gtatctgact
      901 cttctccgtt cctcagacgc ctgtcaagcg tgtcagtgta gtcagtgatg atttccccta
      961 accgtacagg tttaacctcg tcacggggct ttctgatggc tgaaagacgc tttacaagtt
     1021 catccatcgc ctgactcgat gcgtcgatgg ttccgctttg gattggttca cgcatttcat
     1081 ccatgatttc cagcaccaga cggcggtgat agttatccgc gaccattccg gcatatccct
     1141 tcaggtttgc ggcactcggg cagtttttgc tggtcatcag gattgacgtg aaatgctcct
     1201 ctccgcacgc ctcggcaacc atcagcgcat cgattaggtt tctgtttctc gcctgcttgc
     1261 gaataacctc gaaggctttc cggtagagcg gaattgaaaa cgcttccggc tccagcgttg
     1321 ccagaacgtc actggcggtt ggagttaatc caccaatcag caggccaccg ataacgctag
     1381 cttcgatatc ctgtctcatg caatccccct gtctgcaaac ttcccttccc gaactcccgt
     1441 taacgaatct tccctcagca ggtaatcaaa atctgccgtc cagcccgtgt cgttgtctcc
     1501 gaagtaaaac ggcttggcct gatgcacaaa cgccctgaca tacgctctga aaccgtccac
     1561 gtttggcgtt ttcagttgcg ggataatttt cttcaggcgg cgttttcgtt tctcgttgac
     1621 cgcaacagcg tgtggaagtc tgtcaccgac ttcggtgttg taggcgttca ggaaggattc
     1681 gtagtcgatt cgttctgcct tgcgacgttc aggtttaacc tgcccatcgc catccccgtt
     1741 agggggtaag ggggtatttg tatttattgt cttttgtata ttgtcttttg tgtttagctg
     1801 acttggctta tacccattag ccgacttggc taatgtttta ttagctgttt tagctaatgt
     1861 taagctgtcc tggctaatcc actgagaaac caccttgttc actccgattt tcacgccatc
     1921 agcaatgagg aatttacgct caataagctg gcgcttggca gcgcaaacat gagtgtgatg
     1981 aatacctgtc atggctgcta tctgcgtgtt tgtgagtcga tccatcggct tattgaatcc
     2041 gtatgtcttg cgcatgatag cgagcatcac cttcaactgc cggacggtta aatcagccat
     2101 cagcagactg tcggtaatct cgttagcaac gcgcatgaaa ccatcttcgg tatctgccac
     2161 gcgatgctcc acgacctcca gttgaggcct gtaatcagct aacttaacga cgcccatgtt
     2221 tcactcctgc tttggctagt ctgtaaacac caacaaggcg ctctgcgaac gccctgttat
     2281 ttgctgcggc taccactaat ccctcaggtg aatcagggtg tcgaatctct tctttttcct
     2341 ggtatttctt acgacgtttt gtcataatta ctcctgtgga ttgatccagt aattccctca
     2401 gaattgcata tcaatttgct taaaatcctc ggtggcagcc ggggattttt tctttgtgat
     2461 ttcatcaagc gcatacttaa aagccctgct aatcggactg atgtctgatg ccattccgaa
     2521 agcacacaag accgaagcaa taaaccgcca gtccgttctg cttatcttcg attcatgaca
     2581 gccaatcatc tttgccagac cgcgctgtgt aagcgttgac aggttgatga gtaaatctgt
     2641 ttctgcgcga tcaacgtcgc gctgtgatag tttgctgtaa cttgttcgtt ccatttctta
     2701 agatttccaa tagtgaatag ttagttgaaa ggtatgcgtg gaaacgcata tggccttagt
     2761 tggtcagata tcttgggact cgcttttcag cgacgtagga cgaatgtccg ttgttacaaa
     2821 gagcggctcc gcttattaag cggctttgtg ctccggcggg aacacgtcat caagacttac
     2881 ttttgcgcct aacttgttta gacacgcaac aagagcacgg catgttttaa ggtctgggaa
     2941 gcgacgacca gattcccaat gtccgatagc tccctgtgtg catccaactg ccttagcaag
     3001 tgttgtttga gagatattca gtgactctcg atattttcgt aggttgctca tatgccctcc
     3061 atagtaacca cgaataaaaa aatacaatat gtacttctca aatacaagta aaaatacaca
     3121 ttgtgcatgg atggttccag tacagagcgt aataataagg acatgaaaat gaaatggtat
     3181 gaactggcta gatccagaat gaaagagctc ggcataactc aagagaagtt agccgaagag
     3241 ctaggtatga cgcagggtgg aattggacac tggttgcgcg gatctcgtca tccatctctt
     3301 agtgatattg gtgtggtgtt taaatacctt ggtattgata acatatcatt caaccacgac
     3361 gggacatttt cacctgttgg cgaatactca tcggccccag ttaaaaaaca atatgagtac
     3421 cctgtttttt ctcatgttca ggctgggatg ttctctccag aactcagaac ctttaccaaa
     3481 ggtgatgcgg agagattggt aagcacaacc aaaaaagcca gtgactctgc attctggctt
     3541 gaggttgaag gtaactcaat gaccgcacca acaggttcca aacctagttt tcctgacggg
     3601 atgttaattc tggttgaccc tgagcaagct gttgagcccg gcgatttctg catagccaga
     3661 cttggtggtg atgaatttac cttcaagaaa ctgatcaggg atagcggtca ggtgtttcta
     3721 caaccactaa acccgcaata tccaatgatc ccatgcaatg agagttgttc cgttgtgggg
     3781 aaagttatcg ccagccaatg gcctgaagag acgtttgggt aaagaggata gatggagttt
     3841 aatggccatg agtatcaagc ggtcaaaaag aagttcatct attcattgaa agcataaggc
     3901 catctgagta tatccgtaat gatctggata ttgtctataa catcaatgac cggacgatag
     3961 atatcggtga gcagcgtcct gtatggcagg gtgaggcagg tgaaaaaacg tcctgccatc
     4021 aacaagaatc aagtgcatcc gctctctgga cagatggaaa atttattgga tgcaaaaaga
     4081 tatgaaataa cacctgtata gcacggagct ttctctgacc gatgcacttg aagttgttag
     4141 ggctgaccca gcctgctgtt tctttaggta agagaaaaga gatttaggag atgaaaggtc
     4201 gcagaggtgc ggcctttttt attgagagtg gatcttgaac ggaaattctc aagacttgag
     4261 tcttgcatgc aaatcaattc ctggataaac tcgatctgag tcaataactt agagagagag
     4321 caaatggcaa agtcaaacgt tagcgtgcag gcattcaagg acttccttga agagcttatg
     4381 tcgctgaaca taatgaagga ggccaccgct cgaaatttaa aaaactcatc cgctcgcctc
     4441 ttaacggtag tccaagaaga ggaaatgggt gatgttactc agcttgatgt gaatgagctt
     4501 gccgagcgat acatcaacgc aactgagccg aagcctagcg acagcagcat tactgcatat
     4561 aaaagccgca tggaaagtgc aatcaaaaag tttgtagctt tccagtctgg tgaagaaatc
     4621 ccatacactc cgattgacaa agaatccagt gaggaaaaag atttgactgg cgaaccaaca
     4681 aaagtcgaag gcaaggctaa tgcacttcat acctatgatc ttccagtagt tcttcgacct
     4741 gaatcagggg ttacagtaac gattaaaggc attcctaacg atatcacaaa cgaagaagcc
     4801 gaacgcatct cttcaattct gaaggtttac gttcggcctc aataatgcaa aagcattcag
     4861 cacaatgtcc aactccccag tccgacattg atgctgttta accagagcct ccaaaaggag
     4921 cccttgttaa ggtacacaat atttgcgatg taaccttagc gcgtctggta cattttttca
     4981 agcggttgta gggctgccgc caatatgaga aaacacagat gtctacatac aatttgaatg
     5041 accaattcga tcgagaagtt catgtgaatg cctatgaacg gataaggcat ggaaatcttg
     5101 aacatgtgtg tgagcattat cgctcaaggc cacaccgcta atcatcaacc cggcctccat
     5161 gccgggtttt cttttcctct cgcccaaaaa aacacataac caattgtatt tattggaaaa
     5221 taaatagata caactcacta aacaacgcaa ttctgatctc tccttacatc gccgaggcaa
     5281 tacacccacg ctaaaaaaca atactattaa atacaaagcg ttgtaaaaaa acgccccgtt
     5341 ttagaacaaa ttgtattgac caagtgaagt acatatcgta ctatttaacc atcagcagga
     5401 cgctggaagc caaatggaac agactggcag gctctttaaa caacgtcgac tctcgactac
     5461 gtggctgaaa agccagatca cccaaccaca taagctgtgg gatgcaatgc cgaagcaacc
     5521 gtctcaggag gagcttcgag attgcatcgc caaagtttat tcgggaggaa tctatgtcca
     5581 gaaaaacaga atttaaaggc accgcagctt ctcgccgtag agctcgtcgc gcaaacctgc
     5641 aaagtcagga ggcgattagc tccgacaaac tacacaggcc aaccccatca cgagtggtct
     5701 tacaatgcaa acgcaaacca gcaatgagag cagaagtaat aacactgaca acgttgacca
     5761 gaaaatatga aggctcaact tgtcttccaa atgtagctct ttacgcggca ggctaccgta
     5821 aatccaaaca actgacagca agatgacttg tgttggtcgc cagaaaatga aattaggcag
     5881 caaaccactt atttgaggtg atatatggaa tttcatgaaa gtgcgatttg tgattttcgc
     5941 gctaacgcaa attcagtaaa accacagcca attgcagttc tttttaaaac aatgggtgcg
     6001 tgggctgttt tatgcttcgc cgctgacgac actgacgcaa gaatggcaat aggacaagag
     6061 atggagatgg acccgacaaa cgatgaattc ataatttatg gcgctccatc taattactta
     6121 cttgatacct gcaacattta caacaaggct gcctgatggt ggcctttatt tttggcataa
     6181 acaacagaat aaacacagca ctgtgtattc attccaacga gtgaatacac ggagcaatgt
     6241 cgctcgtaac taaacaggag ccgacttgtt ctgattattg gaaatcttct ttgccctcca
     6301 atgtgagggc gattttttat ctgtgaggat atgaacagat gtcaaacatc aaaaaataca
     6361 tcattgatta cgactggaaa gcatcaatag aaattgaaat cgaccatgac gtaatgacag
     6421 aggaaaaact tcaccagatt aataatttct ggtcagactc tgaataccga ctcaataaac
     6481 acggctcttt attaaatgct gtattaatca tgctggcgca acatgctctg cttatagcaa
     6541 tttcaagcga cttaaatgca tatggtgttg tgtgtgagtt cgactggaat gatggaaatg
     6601 gtcaggaagg atggcctcca atggatggta gcgaaggaat aagaattacc gatatcgata
     6661 catcaggaat atttgattca gatgatatga ctatcaaagc cgcctgagcg cggcgttacc
     6721 gcataccaat aacgcttcac tcgaggcgtt tttcgttatg tataaataag gagcacacca
     6781 tgcaatatgc cattgcaggg tggcctgttg ctggctgccc ttccgaatct ttacttgaac
     6841 gaatcacccg taaattacgt gacggatgga aacgccttat cgacatactt aatcagccag
     6901 gagtcccaaa aaatggatca aacacttatg gctatccaga ctaaattcac tatcgccact
     6961 tttattggcg atgaaaagat gtttcgtgag gccgtcgacg cttataaaaa atggatatta
     7021 atactgaaac tgagatcaag caaaagcatt cactaacccc ctttcctgtt ttcctaatca
     7081 gcccggcatt tcgcgggcga tattttcaca gctatttcag gagttcagcc atgaacgctt
     7141 attacattca ggatcgtctt gaggctcaga gctggacgcg tcactaccag cagatcgccc
     7201 gtgaagagaa agaggcagaa ctggcagacg acatgggaaa aggtctgccc cagcacctgt
     7261 ttgaatcact ctgcatcgat catttgcaac gccacggggc cagcaaaaaa gccattaccc
     7321 gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc agaacacatc cggtacatgg
     7381 ttgaaaccat tgctcaccac caggttgata ttgattcaga ggtataaagc ggatgagtac
     7441 agcactcgca acgctggcag ggaagctggc tgaacgtgtc ggcatggatt ctgtcgaccc
     7501 acaggaactg atcaccactc ttcgccagac ggcatttaaa ggtgatgcca gcgatgcgca
     7561 gttcatcgca ttgttgatcg tcgccaacca gtacggcctt aatccgtgga cgaaagaaat
     7621 ttacgccttc cctgataagc agaacggcat cgttccggtg gtgggcgttg atggctggtc
     7681 ccgcatcatc aatgaaaacc agcagtttga tggcatggac tttgagcagg acaatgaatc
     7741 ctgtacatgc cggatttacc gcaaagaccg taatcatccg atctgcgtta ccgagtggat
     7801 ggatgaatgc cgccgcgaac cattcaaaac ccgcgaaggc agagaaatca ccggcccgtg
     7861 gcagtcgcat cccaaacgga tgttacggca taaagccatg attcagtgtg cccgtctggc
     7921 cttcggattt gctggtatct ataacaagga tgaagccgag cgcattgtcg aaaatactgc
     7981 atacactgca gaacgccagc cggaacgcga catcactccg gttaacgatg aaaccatgca
     8041 ggagattaac actctgctga tcgccctgga taaaacatgg gatgacgact tattgccgct
     8101 ctgttcccag atatttcgcc gcgacattcg cgcatcgtca gaactgacac aggccgaagc
     8161 agtgaaagtt cttggattcc tgaaacagaa agcctctgag cagaaggtgg cagcatgaca
     8221 ccggacatta tcctgcagcg taccggaatc gacgtgagag ctgtcgaaca gggggatgat
     8281 gcatggcaca aattacggct cggcgtcatc accgcttcag aagttcacaa cgtgatagca
     8341 aagccccgct caggaaagaa gtggcctgac atgaaaatgt cctacttcca caccctgctt
     8401 gccgaggttt gcaccggtgt ggctccggaa gttaacgcta aagcactggc ctggggaaaa
     8461 cagtacga
//
LOCUS       DABSYT020000154         8372 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.154, whole genome shotgun sequence.
ACCESSION   DABSYT020000154 DABSYT020000000
VERSION     DABSYT020000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.154"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(151..227)
                     /locus_tag="ID725_RS22605"
                     /old_locus_tag="pgaptmp_004521"
     tRNA            complement(151..227)
                     /locus_tag="ID725_RS22605"
                     /old_locus_tag="pgaptmp_004521"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Asp,seq:gtc)
     gene            complement(360..1091)
                     /gene="dnaQ"
                     /locus_tag="ID725_RS22610"
                     /old_locus_tag="pgaptmp_004522"
     CDS             complement(360..1091)
                     /gene="dnaQ"
                     /locus_tag="ID725_RS22610"
                     /old_locus_tag="pgaptmp_004522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGV7852977.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1156..1623
                     /gene="rnhA"
                     /locus_tag="ID725_RS22615"
                     /old_locus_tag="pgaptmp_004523"
     CDS             1156..1623
                     /gene="rnhA"
                     /locus_tag="ID725_RS22615"
                     /old_locus_tag="pgaptmp_004523"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGV7852978.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1620..2342)
                     /locus_tag="ID725_RS22620"
                     /old_locus_tag="pgaptmp_004524"
     CDS             complement(1620..2342)
                     /locus_tag="ID725_RS22620"
                     /old_locus_tag="pgaptmp_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGV7852979.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2376..3131
                     /gene="gloB"
                     /locus_tag="ID725_RS22625"
                     /old_locus_tag="pgaptmp_004525"
     CDS             2376..3131
                     /gene="gloB"
                     /locus_tag="ID725_RS22625"
                     /old_locus_tag="pgaptmp_004525"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGV7852980.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3203..4561
                     /gene="mltD"
                     /locus_tag="ID725_RS22630"
                     /old_locus_tag="pgaptmp_004526"
     CDS             3203..4561
                     /gene="mltD"
                     /locus_tag="ID725_RS22630"
                     /old_locus_tag="pgaptmp_004526"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGV7852981.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(4609..5379)
                     /locus_tag="ID725_RS22635"
                     /old_locus_tag="pgaptmp_004527"
     CDS             complement(4609..5379)
                     /locus_tag="ID725_RS22635"
                     /old_locus_tag="pgaptmp_004527"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGV7852982.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(5457..6257)
                     /locus_tag="ID725_RS22640"
                     /old_locus_tag="pgaptmp_004528"
     CDS             complement(5457..6257)
                     /locus_tag="ID725_RS22640"
                     /old_locus_tag="pgaptmp_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGV7852983.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6498..7412
                     /gene="yafC"
                     /locus_tag="ID725_RS22645"
                     /old_locus_tag="pgaptmp_004529"
     CDS             6498..7412
                     /gene="yafC"
                     /locus_tag="ID725_RS22645"
                     /old_locus_tag="pgaptmp_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGV7852984.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7409..8212)
                     /gene="dkgB"
                     /locus_tag="ID725_RS22650"
                     /old_locus_tag="pgaptmp_004530"
     CDS             complement(7409..8212)
                     /gene="dkgB"
                     /locus_tag="ID725_RS22650"
                     /old_locus_tag="pgaptmp_004530"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGV7852985.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2232 a         1998 c         2044 g         2098 t
ORIGIN      
        1 tgaattttaa tgaatgagac aacaagcgag aatcacgcac ttacgccaga gtaaaatcat
       61 tgaacagaga aatggtggaa ttcaaataca aaaaaaaccg caaaattaaa aatcttgcgg
      121 ttctctgaac tcattttcat gaggtgaatt tggcggaacg gacgggactc gaacccgcga
      181 ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccgcg ttgtgttccg
      241 ttgggaacga ggcgaatatt acggattgcc tcacccttcg tcaacggttt ttctcatctt
      301 ttgaatcatt tgctgcaaaa atcgcccaag tcgctatttt tagcgccttt tacatgtatt
      361 tatgcccgcc agaggcagct tccgcccttt ttctgcacca gatcgagacg ggcttcatgt
      421 gctgcaagct cttcatctgt cgcaaaaaca acgcgtaact tacttgcctg acgtacaagg
      481 cgctggattg ttgcttcacc ttgttgctgt tgtgtctctc cttccatcgc aaaagccatc
      541 gacgtttgac caccggtcat cgccagataa acttccgcaa ggatctgggc atcgagtaat
      601 gccccgtgca gcgttcgttt actgttatct atttcgtagc gggcacataa cgcatcgagg
      661 ctgttgcgtt taccgggaaa cattttcctc gccaccgcaa ggctatcggt gaccttacag
      721 aaggtattag tcttcggaat atcgcgctta agcaacgaga actcgtagtc cataaagccg
      781 atatcgaacg ctgcgttatg gatcaccaac tccgcgccgc ggatatagtc catgaactca
      841 tcggctactt cggcaaacgt gggcttatcg agcaaaaatt catcggcaat accatgtacg
      901 ccaaaggctt ccggatccac cagccgatcg ggtttgagat aaacatggaa gttattgccc
      961 gtcaggcgac ggttcaccac ttcaacggca ccaatctcaa tgatcttgtg gccttcatag
     1021 tgcgcaccaa tctggttcat accggtggtt tcggtatcga gaacgatctg gcgtgtaatt
     1081 gcagtgctca tagcggtcat ttatgtcaga cttgtcgttt tacagttcga ttcaattaca
     1141 ggaagtctac cagagatgct taaacaggta gaaattttca ccgatggttc gtgtctgggt
     1201 aatcctggac ctgggggtta cggcgctatt ttacgctatc gcggacgcga gaaaaccttt
     1261 agcgcaggct acacccgcac caccaacaac cgtatggagt tgatggccgc tattgtcgcg
     1321 ctggaggcgt taaaagaaca ttgcgaagtc attttgagta ccgacagcca gtatgtccgc
     1381 cagggtatca cccagtggat ccataactgg aaaaaacgtg gctggaaaac cgcagacaaa
     1441 aaaccagtaa aaaatgtcga tctctggcaa cgtcttgatg ctgcattggg gcagcatcaa
     1501 atcaaatggg aatgggttaa aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa
     1561 ctggctcgtg ccgcggcgat gaatcccaca ctggaagata cgggctacca agtcgaagtt
     1621 taagcctgtg gtttacgaca ttgccgggtg gctccaaccg cctggcgaat tcgtggcttg
     1681 tttttactct gtttcatcgg atttagcgtt aaaggaatag tccgtttccg ggcaacaata
     1741 agttgtaagc aaccaagcgc aggaatatgc gcattcaaaa gttttcctcc gtgtttgttc
     1801 cacgggagaa cgtggaaacg gctggcgtgt agcacttcaa aattcaacaa agagagccag
     1861 tccagttgcc gcatcagagt aaacatccgg ctgttatagg gcgaggtttt gcgcaatacc
     1921 ggcacaagtt tgcgtaatcc cattaaacta atgggattga agccactaat gaccagccag
     1981 ccatcatcaa tcaatacccg atcggcttca cgcaataaac gatgcggatc ggtgcaccac
     2041 ggcaatgtat gtgccagtag acaaacatca acggatttat cggcaaaagg aagatgaagt
     2101 gggtccgcct gcacctggac aggcattcct tgcgcagaaa cattcacttg atgagaaacc
     2161 gcgcacgctt cgcaattgat ttctgcgctt aaattgccaa tcttaagcag ataaaaacca
     2221 tacattttag tgaaccacgg gttgagctgg cgctccagcg ccttgcgata aagctctccc
     2281 cagggcaaat cgccccagcg atcaggagcc acgacagttt gagggactct tgccggtttc
     2341 atcacaacct tccgtttcac actgagaggt aatctatgaa tcttaacagt attcccgcct
     2401 ttgatgacaa ttacatctgg gttttgaatg atgaagcagg tcgctgcctg attgtcgatc
     2461 ccggagacgc agagccagta ttaaacgcca ttgccgccaa taactggcaa ccggaggcca
     2521 tatttctcac ccaccatcat cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt
     2581 ttccgcaaat tgtggtgtat ggtccacaag agacacaaga taagggaaca acacaggtag
     2641 tcaaagatgg cgaaactgcc ttcgttttgg ggcatgaatt tagtgtaatt gccacgccgg
     2701 gtcacacttt aggacatatc tgttacttca gtaaacctta tctattttgc ggcgacacgc
     2761 tgttttctgg tgggtgtggt cggttgtttg aagggacagc atcacaaatg tatcaatcac
     2821 ttaaaaagtt aagtgcgtta cctgacgata cattggtatg ttgtgctcat gaatatacgt
     2881 tatcaaatat gaagtttgct ttgagtattc ttccgcacga tttgtccata aatgattatt
     2941 atcgtaaagt taaggagtta cgggcaaaaa atcaaataac actacccgta attctgaaaa
     3001 atgagcggca aattaatgtt tttttaagaa cggaagatat tgatttaatt aatgtaatta
     3061 atgaagaaac attattgcaa caacctgaag agcgttttgc atggttaagg tcaaagaaag
     3121 ataggttctg ataaaacttt cttgtcatcg gctccgttcg ccgttatgat cggtcgtctt
     3181 ttaagcaact attgacgcac acatgaaggc aaaagcgata ttactcgcct ctgtcctgct
     3241 cgtgggttgc cagagtaccg gcaacgttca acagcacgca cagagccttt ctgcagctgg
     3301 tcaaggggaa gcagcaaagt ttacaagtca ggcacgatgg atggacgatg ggacgtctat
     3361 cgcgccagat ggtgacttgt gggctttcat tggcgacgag ctaaagatgg gaattccgga
     3421 aaatgaccgg attcgcgaac agaaacagaa atatttacgc aataagagct atctccacga
     3481 tgtaacttta cgggcagagc cgtatatgta ctggatagcc gggcaagtta aaaaacgtaa
     3541 catgcctatg gaactggtac tactacccat agtggagagc gcttttgatc ctcacgcaac
     3601 gtctggcgcc aatgccgcag gcatctggca gatcattccg agcacggggc gcaattatgg
     3661 tttgaaacag acccgcaatt atgacgcgcg tcgcgatgtt gttgcttcaa caactgccgc
     3721 gctgaatatg atgcagcgtc tgaacaaaat gtttgatggc gactggcttc tgaccgtagc
     3781 ggcttataac agcggcgaag gtcgggtcat gaaggcaatt aaaacgaaca aagcgcgtgg
     3841 gaaatccacg gacttctggt cgttaccgtt gccgcaggaa acgaagcagt acgtgcctaa
     3901 aatgctggca ttgagtgata ttctcaaaaa cagcaagcgt tatggcgtac gtctgccaac
     3961 gaccgatgaa agccgtgctc tggcgcgtgt gcacctgagt agcccggttg aaatggcgaa
     4021 ggttgcagat atggcgggga tttccgtcag caagctgaag acattcaacg ctggcgtgaa
     4081 aggctccacg ctgggcgcaa gtggtccgca gtacgtgatg gtgccaaaga agcatgcaga
     4141 tcaactgcgt gaatctctgg cttcaggcga aattgctgct gtacagtcga cgctggttgc
     4201 cgacaatacg ccgcttaaca gccgtgttta caccgtacgc tctggcgaca cgctttcaag
     4261 tatcgcttca cgtctcggcg taagcaccaa agatttgcag cagtggaaca aactgcgcgg
     4321 ctctaagctg aaaccaggcc aaagtctgac gattggcgca ggtagtagcg cacagcggtt
     4381 ggcaaacaac agcgatagca ttacgtatcg tgtgcgcaaa ggcgattcgc tttcaagcat
     4441 tgctaaacgc cacggcgtga acatcaaaga tgtgatgcgc tggaacagcg atactgcgaa
     4501 tctgcaacca ggcgataagc tgacgttgtt tgtgaaaaac aacagcatgc cagactcctg
     4561 acaaaccaga taatagaaag gcaccgattc ccccggtgcc ttttttattt atgccgcttt
     4621 atgtgcatct accatgatga tatcactggt gaaagagcca tcattctgca aggcaaaata
     4681 cgttctcacc tctgtcgatg cgctctgttg gtaaatgcga atagcgtcta ctaacgcttc
     4741 tggcgtacgc attctcgcga cccatgaaga aaattccagc ggtaacttat ctgtaattaa
     4801 attatcaact atcagattgg cttcattgat taacctcaac cactcaccgc tggcgtagtt
     4861 tcgtacgtga gaggtatcgc ataatgcttc taccgtctgt aaccagatgt cgcgcactgg
     4921 gtggcccgga gacattacgt ccatcacaat cagcctaccg ccaggtttca atatccggtt
     4981 cacttcacgc agtgctgcac caacatcatg ccagtgatgg gcagaataac ggctgataac
     5041 aatatcaaat gcgttatcgg caaatggcag actttcggca tacccctggc gggtggtgat
     5101 atttttcagt tgccggactt cggcagcttg tgccacgaca tccagcattt gggcagataa
     5161 gtcatacgcc accaccgtgc tcacattttg cgcagcgaca aagctggcat gccctgctcc
     5221 gcagcccata tcaagtacac ttgcatcagg ataatcagcc agacacaccg ccaggagctg
     5281 taaatctcgt ccggatgcat gtacggtact ggtcagatat tcgctggctt gagagctaaa
     5341 ctgcttttct acgtggtcat ggtgggattg tgttgtcatt gcactgtcct tttgttgttt
     5401 gagtataaaa gggcagctcc aaacgttggt ctgccctgtg gcagacctga cataccttat
     5461 ttatcaggct tgccgggact gaattcaacg agtagcggat tgtgatcgga cgcgcgcgta
     5521 accagtacag aagcttcgct gacgttcaga ccacggtaga aaacaaaatc gagcgggcga
     5581 ccaaacgccc gacggcgctg atcatcggta aaacgcacct gacgcagcga catttcccgc
     5641 gcaaaacgat ataacgcgtt catccttcta cggctccaag cattgaaatc tcccgccata
     5701 atgaccgggc cgctgtggtg agctatctga tcaccaatag gaagtaactg cttactataa
     5761 acatccacgc ccagactgaa gttgacggcg tgtatattaa ccaccattaa caggcgggtg
     5821 tcaggtaatg gatagaccgt caccagtgcc gacttcgcca gacgcaaaat gggttctcgt
     5881 tcacgtaacg ggcagcaata cactggatgt gccgccgaaa gggtcattac gccagaagga
     5941 tgttgtggca gcacgaaagc gggtacctga tcggcggcaa gatagttagc agtcgcaaac
     6001 tgtaccaact ctggcgttgt ttgcgcttcc tgcaataaca ccagatgtgc gtctttgccg
     6061 taattcttta ataccgacaa ccattcagcg cgttgctgtt tgtatatgtt ccacaccagg
     6121 atccgaatac gctcttcggt acttaacggt tctccgggtg gtaatgcctg gccgatgctc
     6181 gcaaaagacc ccggcggtaa gatcctctcc gcaggttgtc cggcaacata gcgcatggca
     6241 taggtgtttt ttcgcacttt tgactttgaa acctctatta cgtcaaccct tccgcactcg
     6301 ggagggttct actgttatag ggattttagc tcactctttc aacgagcaat tgtatattgt
     6361 tatgtaagca agtgcttaca ccggtcagaa ttttgctcat tattgagaac attaaatcgg
     6421 atgacatatc acgcatcctg atggtttaat gacaaataaa ttcaaaatat ctcatctatt
     6481 ttgcaacagg agcaaaaatg aaagccacgt cggaagaact cgccattttt gtttcggtcg
     6541 tcgaaagcgg cagctttagc cgggcagcgg aacaattagg gcaagcaaac tcagcggtaa
     6601 gccgggcggt gaaaaagctg gagatgaaac ttggcgttag cctgcttaat cggaccacgc
     6661 ggcaacttag cctgacggaa gaaggcgagc gttattttcg tcgcgtacag tcaattttgc
     6721 aggagatggc agcggcagaa tcagaaatta tggagacgcg taatacaccg cgtggactgt
     6781 tgcgcatcga tgccgcaact ccagtggtgc tgcactttct gatgccgttg attaagcctt
     6841 ttcgtgaacg ctatccggaa gtcactttat cgctagtctc ctccgaaacg attattaatt
     6901 tgatcgaaag aaaagtggat gtcgcgatac gcgccggtac gttaacggat tccagcttac
     6961 gtgccaggcc gttatttaac agttatcgaa aaattatcgc ctcccccgat tatatttccc
     7021 gctacgggaa gccagaaacg atcgacgatt taaagcaaca tgtttgcctg ggattcactg
     7081 aacccgcttc cctcaatacc tggccggtag cctgtagcga tggacaatta catgaggtga
     7141 agtacggttt gtcatccaat agtggggaaa cactgaaaca gctttgcctg agtggcaacg
     7201 ggattgcgtg tttgtccgac tatatgatcg acagagaaat cgctcgcgga gaattggtgg
     7261 agttaatggc agataaagtg ttgccagtgg aaatgccatt cagtgccgtc tattacagcg
     7321 accgtgcggt aagtacgcgc atccgggctt ttatcgattt ccttagcgag catgtaaaaa
     7381 cagctcccgg aggagctgtc agagaggctt aatcccattc aggagccaga ccttccgggc
     7441 taaccaggcg gtcgttgcaa tccagtgcgg cgatcgcttt tttatcttcg gcatcaagct
     7501 gtaaattttg tgccttaaga ttactctcca ggttttcacg tttagtagaa gaaggaatta
     7561 ctgagtaacc ttcccccata gcccacgcca gaatcacttg tgccggagtc gcattgtgtt
     7621 tagctgcgat acgagcaata acctcatctt tcagggcctt accatacgcc agcgtcatat
     7681 aggaagtaat atggatgccg tgctgtttag cccaggcaac cactttacgg ttttgcagat
     7741 aaggagagag ttcaatctgg ttagtagcga tgttttcagc gccaacagca gcaatcgcct
     7801 tttccatcaa tgggatcgtg aagttggaaa taccgatctc acgcgtcaac ccttcttttt
     7861 tggcttccag cagcgcctgc ataaactctt caacagagac ttcatcgttt ggtgacggcc
     7921 agtggattag cgtcagatca acataatcgg tacgcaattt ttgcaggctc tcttttagac
     7981 tcgggatcaa tttgtctttg ctgagatttt caatccagat tttagtggtg atgtagagtt
     8041 catgacgtgg cacgccactt tctgcaatcg ccagacctac tgcggcttcg ttatcataga
     8101 tttgtgcagt atcaattgcg cgataaccaa gttcaagcgc cgttttcaca gatgaaataa
     8161 caacgtcgtc tttcagacgg aaagtaccta aaccaaatgc agggatagcc ataatatgcc
     8221 tcttttaatg ctattttcgt taactgaggt gattatgggc tttagaatgt tgcggaaaaa
     8281 gggcgtaaaa cgcagaggat ttttgcgatt ttagcaataa tagaaatgta gacgaaaaaa
     8341 aacctcgagc gataactcga ggcttcttaa ta
//
LOCUS       DABSYT020000155         8187 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.155, whole genome shotgun sequence.
ACCESSION   DABSYT020000155 DABSYT020000000
VERSION     DABSYT020000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.155"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(130..891)
                     /gene="kduD"
                     /locus_tag="ID725_RS22655"
                     /old_locus_tag="pgaptmp_004531"
     CDS             complement(130..891)
                     /gene="kduD"
                     /locus_tag="ID725_RS22655"
                     /old_locus_tag="pgaptmp_004531"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGV7852986.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(921..1757)
                     /gene="kduI"
                     /locus_tag="ID725_RS22660"
                     /old_locus_tag="pgaptmp_004532"
     CDS             complement(921..1757)
                     /gene="kduI"
                     /locus_tag="ID725_RS22660"
                     /old_locus_tag="pgaptmp_004532"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGV7852987.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2044..3225)
                     /gene="yqeF"
                     /locus_tag="ID725_RS22665"
                     /old_locus_tag="pgaptmp_004533"
     CDS             complement(2044..3225)
                     /gene="yqeF"
                     /locus_tag="ID725_RS22665"
                     /old_locus_tag="pgaptmp_004533"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGV7852988.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3480..4709
                     /gene="yqeG"
                     /locus_tag="ID725_RS22670"
                     /old_locus_tag="pgaptmp_004534"
     CDS             3480..4709
                     /gene="yqeG"
                     /locus_tag="ID725_RS22670"
                     /old_locus_tag="pgaptmp_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGV7852989.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5169..5801
                     /gene="yqeH"
                     /locus_tag="ID725_RS22675"
                     /old_locus_tag="pgaptmp_004535"
     CDS             5169..5801
                     /gene="yqeH"
                     /locus_tag="ID725_RS22675"
                     /old_locus_tag="pgaptmp_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7852990.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            6135..6944
                     /gene="yqeI"
                     /locus_tag="ID725_RS22680"
                     /old_locus_tag="pgaptmp_004536"
     CDS             6135..6944
                     /gene="yqeI"
                     /locus_tag="ID725_RS22680"
                     /old_locus_tag="pgaptmp_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGV7852991.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRG"
     gene            6941..7420
                     /gene="yqeJ"
                     /locus_tag="ID725_RS22685"
                     /old_locus_tag="pgaptmp_004537"
     CDS             6941..7420
                     /gene="yqeJ"
                     /locus_tag="ID725_RS22685"
                     /old_locus_tag="pgaptmp_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HGV7852992.1"
                     /translation="MIIKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIFN
                     DQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWVENYDTAHLTSK
                     KVNKFEIMDQVDDDRLAELIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
     gene            complement(7453..7533)
                     /locus_tag="ID725_RS22690"
                     /old_locus_tag="pgaptmp_004538"
     CDS             complement(7453..7533)
                     /locus_tag="ID725_RS22690"
                     /old_locus_tag="pgaptmp_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852993.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(7569..7994)
                     /gene="yqeK"
                     /locus_tag="ID725_RS22695"
                     /old_locus_tag="pgaptmp_004539"
     CDS             complement(7569..7994)
                     /gene="yqeK"
                     /locus_tag="ID725_RS22695"
                     /old_locus_tag="pgaptmp_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HGV7852994.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT         2433 a         1812 c         1619 g         2323 t
ORIGIN      
        1 ataaaattca taaagttcat taattgataa ttgatatgga ttatttcata accatgatat
       61 ggattatgat gatctagaga tataaaaaac cctgccatgc ggcagggtca taaaagtaag
      121 aggaatgaat taacgcgcca gccaaccgcc atccacagca atggtataac catttacata
      181 atctgaagcg ctggaggcaa gaaacactac cggccccatc aggtcactcg gcagtcccca
      241 acggccagct ggaatgcggt cgagaatttc cgcgctacgt tgttcatctg cccgcagttg
      301 ttgggtattg ttggtcgcca tgtaacccgg agctatcgca ttaacattaa tgttgtgttt
      361 agcccattca ttcgccatca atcgcgtcac gcccatcacg ccgctttttg atgcggtata
      421 agaaggcaca cggatcccgc cctggaagga gagcattgac gcgatattga taatcttgcc
      481 gccattgcct tgcgcgatga agtgtttcgc cgctgcctga gacatgaaga atacgctctt
      541 gatattcagg ttcatgacat cgtcccagtc cttttcgctg aactcgagag catcttcgcg
      601 gcgaatcaat ccggcgttat tcaccaggat atcaatatga ccaaactcag ctaccgcgcg
      661 atccagcagt ccaggaatac catcaatctt tcgcagatcg gcggtcaggc ttaaaaaacg
      721 acgccccagc gccgtgacct gcttgatggt ttcagtcggt tcaacgatgt taatgccaac
      781 aatgtcacag cccgcttgcg ccagccccaa cgccatcccc tggcccagcc cagtatcaca
      841 accagtgacg accgcaactt taccttcgag agaaaatgca cttaaaatca taacaatacc
      901 ttattcgttt atgcccacaa ctagcgcaaa tctttaacgg ccacatggtc catatcatca
      961 aagacctggt tttcaccgac catgccccag ataaaggtat aagctttggt tccgacgccg
     1021 gaatgaatcg accagctcgg ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc
     1081 gtttcttgcg gctgccccat catgtggaaa acgcaggcgt catcatccat attgaaatag
     1141 aaataaactt ccatccggcg ctcgtgggtg tgacacggca tggtgttcca caagttaccc
     1201 ggagccagct ccgtcagccc catactcaat tggcaggttt ccagcacatc cgggacaaaa
     1261 tatttgttaa tcgtgcgacg gttactggtg aggttatcgc ctaacgtgac tggagatact
     1321 tcgtccggtg tgactttttt ggtaggatac gtcgtatgcg cgggtgcgca attgtaataa
     1381 aacttcgccg gagtgccggt atcaatacta gcaaagacaa cttcttttgc gcctttaccc
     1441 acatacaggg cgtcgcggtg accgatttca tagcattgac catcgacagt aatcgtaccg
     1501 gcaccgccaa tattgataac acctaactcg cgacgttcaa ggaaataact tacgccgagt
     1561 tgtttaccca cttccccgcc aacggaaacc gttttagtta ccggcataat gccgccaata
     1621 ataatacggt caatgtggct gtaaaccatg gtgtactcat cggcgacaaa taccttttca
     1681 accaaaaatt cattgcgcag cccctgggtt tccagcgttt ttgcgtgcgc actgtggatg
     1741 ctctgtctta cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat
     1801 agtgaaacac tgtttcgttt tctctataag gggatgatat gcccatggga tcgggttttc
     1861 aattaaaaat aaaacaatgt ttcattttta agttaaggat taaaaaaagt gcagtgtcga
     1921 tcacgaatct gtcatgagaa tggaaaaatc agggagcccg gaaaatgtat gtcgacttgc
     1981 cagccatcat tattcaacag attaatgacg gcagatattt atcggtaagg aggaaatgca
     2041 gagctattcg tcacgttcaa tggtcaatgc cacaccctgg cccccgccga tacaaagcgt
     2101 tgccagtcct ttgcgggcat tacgtttcac catttcatga accagagaaa ccaggattcg
     2161 gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga cattgacccg
     2221 acgctcatcc cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt
     2281 agcctcgata agatcaacat cagccaactg ccagcctaca cgctccaggc aacggcgggt
     2341 cgcatacacc ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg
     2401 gatgcgggcc agcacgggta aattcaacgc tcgtgctttg gcttcgctca tcatcattac
     2461 cgcagctgcg ccatcgttta tggatgatgc attacccgct gtcacagaac cgagactatc
     2521 aaatgaagga tttaaacggg ctaaaccttc tgcactggcg tcagtgtgtg gctgttcatc
     2581 ggtatcaaca accaacgtct gaccgttact ttgggtcatt accgggacga tctcatcttt
     2641 aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc
     2701 ctgcaattga cggctgatgc catattcgcg agccagattt tcggcggtga caccaatatg
     2761 ataatcattg aaggcatccc ataacccatc atgcacaaga ctgtcaacca gctggctatt
     2821 gccaagctgt gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc
     2881 ctggccaccg gcgatgacaa tatcagcctc gccacactgt atcgcctgag tagccagatg
     2941 cagtgcttta agcccggaac cgcagacgtc attaatcgtg attgcagaaa cgctattagg
     3001 cagaccacct ttaatagccg attgccttgc cggattctgc cctgcccctg cagtcaacac
     3061 ctgaccaaga attacttcat ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc
     3121 tttcacgacc agactaccaa gttccacggc ggaatgaccc gctaacgcac cgcgaaagca
     3181 gccgataggt gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa cctcgcacca
     3241 attaacagtg caatagtaaa tgattgttaa ttacagttat cttaattgtt taaaaaaagt
     3301 gatttttatc acaaaggaaa tatacctgag cagtagtcag agtcataact ggcacgtaag
     3361 gtttgcaacc actaccccac caatagaggg gtagataggg cgttaatctc ccatacttaa
     3421 cctggtttat ggtaaattgc cctccatttt gtttaatttg tagatgatac gttcagataa
     3481 tgtctaatat ttggtctaaa gaagaaactc tgtggagttt cgcgctctac ggcacagccg
     3541 ttggtgcagg cacgctcttc cttcctattc agttaggttc ggcaggggct gtggtcctgt
     3601 ttattactgc tctggtcgcc tggcctttaa catactggcc acataaagct ttatgccagt
     3661 tcatcctctc atcgaaaaca tcagcaggtg aagggataac gggcgcggta acacactact
     3721 atggcaagaa gattggtaat ctgattacca cgctgtactt catcgccttt tttgtcgtcg
     3781 tgttgatata tgcagtggca attaccaact cacttacgga acagctggca aagcatatgg
     3841 ttattgatct tcgcatccgt atgttggtga gtctgggtgt tgtattaatt ctgaatctca
     3901 tttttctgat gggacgtcat gccactattc gggtaatggg atttttggta ttcccattga
     3961 ttgcctattt cttatttctt tccatttacc ttgtaggtag ttggcaacct gatctattaa
     4021 caacccaggt agagttcaat cagaataccc ttcaccagat atggatatcg attcccgtga
     4081 tggttttcgc ctttagccat acgcccatta tttctacgtt tgccatagac agacgtgaaa
     4141 aatatggcga acacgctatg gataaatgca aaaaaattat gaaagtcgct tatctcatca
     4201 tctgcataag tgtactgttc tttgtcttta gctgcctgct ttctattcca ccttcgtata
     4261 ttgaagcggc taaagaagaa ggggtcacca ttttatcggc gctttctatg ctgccgaacg
     4321 ccccagcatg gttgtcaatt tccgggatta ttgtcgcagt agttgcgatg tcgaaatcat
     4381 tcctgggtac gtactttggc gttattgaag gtgccacgga ggtcgtcaaa acaacactac
     4441 agcaggttgg tgtaaagaaa agtcgtgcat ttaaccgcgc actatcaatt atgttggtat
     4501 cgctgattac cttcattgtt tgttgcatta acccgaacgc gatttcgatg atttatgcga
     4561 tcagcggccc gctcattgcc atgatacttt tcatcatgcc tacgctatca acatatctca
     4621 tcccggcgct taaaccctgg cgctccatcg gaaatctgat tacgctgatc gtgggtatcc
     4681 tgtgcgtatc ggtaatgttc tttagctaat atccattgtg aatggttcaa aattgctaca
     4741 attttgaacc attccagttt tcataatgtg catttgttat tattccgctc tatcacttct
     4801 ctcgtcatat cctagtatta tcagttagtt aaacacacaa agagatctga acatcttaaa
     4861 taagactatt taagatgcat aacttagatt cgcaagatat cttctggtca ttcaaaaaca
     4921 atttgcgata tagatattct cataccatga gatagttatc tgaaccaccc tcaaccaggt
     4981 aagattaaat cagtgaaaca taatcatatt taataacgag aaatgcattt taaaatgtct
     5041 taatcagaat tatagcaaat acagattaat cgcaattttt cccagcaaaa ttacaaaatc
     5101 atggtgtatt gacatcatca atcaccaatg aaattatgca attatatacg gatagggagg
     5161 ttcttaacat ggggctttgt agtcgttata aaagtcttac atgcaatagt tgctcaatgc
     5221 attgccaaat aatgccagaa gagtcaccgc gtttgcagta ctgcgcaaac tcgtgttttt
     5281 gtatgtggac cgaagaaagt tcatatttta atcgtggtgt ggtagaaggt atcttaacaa
     5341 aaaaccacaa tgcgagatta agcggatata tttttgtcga tttttcagta agttttttac
     5401 gtctattcct tgaaaaagac tggattgact atcttgcaag tactgacatg gggattgttt
     5461 tagtcagcga ccgcaatatg caatcgttag ctaattactg gcgaaaacac aactctgcaa
     5521 tatctgctgt catatataat gatgatggtc ttgatgttgc caatgaaaag atcagacaac
     5581 tatttattgg tcgttattta tcatttacca gaggtaacac attaactcag atggaattca
     5641 ccataatggg gtatatggtt tctggttata atccatatca gattgctgaa gttctggata
     5701 tggatatccg tagcatctac gcgtacaagc aacgaatcga aaagagaatg ggtggtaaaa
     5761 taaacgaatt atttattcgt tcacattcgg tccaacattg atactcaaca atctgccaga
     5821 atcatactct gtttatacgg gcatttattg aaatgcccgt attttataaa taggccgtcc
     5881 cctcaagagt aaacaccata gtatttaaaa ccatcacacg aaaaattcag aagcattacg
     5941 aaatatgaac ttacaaacaa tagtggcata aatattaacc atgttaattt acataaaatt
     6001 ttacgttgcg acattaaagc ctcatttcaa tcatcatgat aaatataaaa ttaatatata
     6061 tttatgccgt aaataccaat atacttagca aactatgtga tttccatttc gattgattta
     6121 gtgtttattg acgtatgtac tggattatta acgataatat cgagttctgg cctgagcacc
     6181 gaaaattaat atcggtacat aacgccgatc ttaacgtcgt tctgacaacg ccagccagtc
     6241 gttgtttatc actgctactt gaagcttttc ctgatgtggt tgcacaacaa gattttttca
     6301 ccagagtctg ggaagaagaa ggtatgcgtg tgcctactaa cacgttatat cagaacatat
     6361 ccattatcag acgcggattt cgcgctgttg gtgatactac ccactcgctt attgcaacag
     6421 tgccgagaag aggattcaag atccataatg acattaacat acaaaatcat gtaataaact
     6481 catcaacaga cgcacataca cacaatgccc cacctgccat aaaagttaat gcgggataca
     6541 aagagagcat tggtggcgca aagaatttca ataacaaaat cctcaaacat ataaaaagtt
     6601 atctaattat gttgagcgca tttgtcatag gcgcatattc tgcatattgg ctatggaata
     6661 ataatcaacc gaagccattt ttcaaggatt acaagactgt tgcggaaatt aatggctgcc
     6721 attttaatgt aacagaagat acaattgatg ggttgaaaga gttcgataaa tataaaacac
     6781 ggatactgga ttcggggatt aactgcaaaa aatatccgtg gttatacttc cctcttgcaa
     6841 aatcctcacc ggggatgatt gttatggcat gcaataaaaa ctataaccaa catgaagtgg
     6901 caggctgttt aaccctgtct taccgtgagg ttaatcgtgg ttgattataa aaaaaaactt
     6961 attatttatt ttagttttta tcagtgggtt tattctcttc actgtctatt cttacactgc
     7021 agaaaaaatg atatataacg aaacatgcac tgcaaattgg gtaattttta atgaccaggg
     7081 acgagcaaat cttaccatag actttatgta taacaaaaaa aataaaacag gaactgttgc
     7141 gttgagtgga acctggcaac aaggtaacag agaaagtaaa tcaatacggc gaaatattga
     7201 atacacatgg gttgaaaact atgacacagc ccatttaaca tcaaagaaag ttaataagtt
     7261 tgagattatg gatcaagtcg atgatgatag acttgcagaa cttattcctg atttttatgt
     7321 ctttccagaa aaaagtgtaa gctataatat actaaagcaa ggtaagcatg cttttatttt
     7381 gagcattggt aacagagcaa taatgcattg tgcaaggtaa actaaaatag ataacaaaaa
     7441 gatggaacct cgttaatcat aaaacaataa aattaaatat tctcgcagta tatggcagtc
     7501 taaagcatca aagttttgat caacatcttt cattttagac atctccttac aatgtaaaat
     7561 atcatgtatc aacaacatct ggtgcaaatc catgagtcga actcgacatt catcttagct
     7621 cgattattat tccttcgtac agtcgatgta aacaatacag aaagaggatt attaagaacc
     7681 agtttactca gtgaatcaat agaggaaagg actaacgttt ctttaaaaga attgatttca
     7741 tcatctgtta aactaaactc atcattgaca gatcgtgaga tataactgtt tttaacttta
     7801 ctctttacgt tgactttatt gacagaatta acattcacaa atcttgaatt taatgcccat
     7861 gatgttgttt gcgtgagaac cttctcagca ttaaggaatt tttttaacgg aatccttggt
     7921 ttctttttcg agtccgaatt tacaatatca tgcatataga ccatttcatg aatctgcgaa
     7981 aactcaatgt ccattatata cctcacattt ttaccgtgac tcgatgttac tgttcaataa
     8041 tcaccttcca tcaatactaa aattaatacc cctaatgtgc cgataacaaa tatagtcatt
     8101 ctacgtaacg tctccataag gtgataacct tgatcaaatg aatttcgaat ataataaagg
     8161 aaatcttttg agatatttat cacgaat
//
LOCUS       DABSYT020000156         8184 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.157, whole genome shotgun sequence.
ACCESSION   DABSYT020000156 DABSYT020000000
VERSION     DABSYT020000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.157"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(68..259)
                     /locus_tag="ID725_RS22700"
                     /old_locus_tag="pgaptmp_004540"
     CDS             complement(68..259)
                     /locus_tag="ID725_RS22700"
                     /old_locus_tag="pgaptmp_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGV7852995.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(256..444)
                     /gene="dicB"
                     /locus_tag="ID725_RS22705"
                     /old_locus_tag="pgaptmp_004541"
     CDS             complement(256..444)
                     /gene="dicB"
                     /locus_tag="ID725_RS22705"
                     /old_locus_tag="pgaptmp_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGV7852996.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1018..1203
                     /locus_tag="ID725_RS22710"
                     /old_locus_tag="pgaptmp_004542"
     CDS             1018..1203
                     /locus_tag="ID725_RS22710"
                     /old_locus_tag="pgaptmp_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852997.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(1390..1779)
                     /locus_tag="ID725_RS22715"
                     /old_locus_tag="pgaptmp_004543"
     CDS             complement(1390..1779)
                     /locus_tag="ID725_RS22715"
                     /old_locus_tag="pgaptmp_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7852998.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ID725_RS22720"
                     /old_locus_tag="pgaptmp_004544"
     CDS             complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ID725_RS22720"
                     /old_locus_tag="pgaptmp_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HGV7852999.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(1921..2076)
                     /locus_tag="ID725_RS22725"
                     /old_locus_tag="pgaptmp_004545"
     CDS             complement(1921..2076)
                     /locus_tag="ID725_RS22725"
                     /old_locus_tag="pgaptmp_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGV7853000.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2354..2641)
                     /locus_tag="ID725_RS22730"
                     /old_locus_tag="pgaptmp_004546"
     CDS             complement(2354..2641)
                     /locus_tag="ID725_RS22730"
                     /old_locus_tag="pgaptmp_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGV7853001.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(2641..2832)
                     /locus_tag="ID725_RS22735"
                     /old_locus_tag="pgaptmp_004547"
     CDS             complement(2641..2832)
                     /locus_tag="ID725_RS22735"
                     /old_locus_tag="pgaptmp_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGV7853002.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(2860..3261)
                     /locus_tag="ID725_RS22740"
                     /old_locus_tag="pgaptmp_004548"
     CDS             complement(2860..3261)
                     /locus_tag="ID725_RS22740"
                     /old_locus_tag="pgaptmp_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7853003.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            3370..3642
                     /locus_tag="ID725_RS22745"
                     /old_locus_tag="pgaptmp_004549"
     CDS             3370..3642
                     /locus_tag="ID725_RS22745"
                     /old_locus_tag="pgaptmp_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGV7853004.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            3626..4051
                     /locus_tag="ID725_RS22750"
                     /old_locus_tag="pgaptmp_004550"
     CDS             3626..4051
                     /locus_tag="ID725_RS22750"
                     /old_locus_tag="pgaptmp_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGV7853005.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4258..4713)
                     /locus_tag="ID725_RS22755"
                     /old_locus_tag="pgaptmp_004551"
     CDS             complement(4258..4713)
                     /locus_tag="ID725_RS22755"
                     /old_locus_tag="pgaptmp_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853006.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            4792..5889
                     /locus_tag="ID725_RS22760"
                     /old_locus_tag="pgaptmp_004552"
     CDS             4792..5889
                     /locus_tag="ID725_RS22760"
                     /old_locus_tag="pgaptmp_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGV7853007.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMT
                     AGSEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANP
                     KKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFEN
                     PWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            5896..6642
                     /locus_tag="ID725_RS22765"
                     /old_locus_tag="pgaptmp_004553"
     CDS             5896..6642
                     /locus_tag="ID725_RS22765"
                     /old_locus_tag="pgaptmp_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGV7853008.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGLKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            6664..7434
                     /locus_tag="ID725_RS22770"
                     /old_locus_tag="pgaptmp_004554"
     CDS             6664..7434
                     /locus_tag="ID725_RS22770"
                     /old_locus_tag="pgaptmp_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGV7853009.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            7450..7863
                     /locus_tag="ID725_RS22775"
                     /old_locus_tag="pgaptmp_004555"
     CDS             7450..7863
                     /locus_tag="ID725_RS22775"
                     /old_locus_tag="pgaptmp_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGV7853010.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         2202 a         1740 c         2122 g         2120 t
ORIGIN      
        1 agggtgaaca aatccctgcc attgctggca tataaaaatg aaaccggata ttaattacgg
       61 tgctgtttta aagtcctgcc ggtatttcgt tattattagt gtgagtagtt ttatctaccg
      121 gataacagtt accgggaatt ttttgttctg ctgctgcagc catgcattct ttcattgaac
      181 cgtataagcc agtcaccagc tcaagagatt cgccggaaac aagataaact gtcagaacga
      241 gtgcaaatgt tgtattcatt gtttatatcc tttttgcagc aggtccagac gagccagcat
      301 tgaaggaatg catacttcat ttaacaggtc ctgctcgagt tttctctgct taatggcgtc
      361 ttcaataaat gttttgtctc cagtgataac gccaatttcg aaacgaagtt cagacgtgct
      421 ggcattacat gataactttt ccattatcgc gtcctcaaca atgaattttg tgatgcggtg
      481 cctggtgcct ccaggtgacg ttaaccagtt aacaattaac gccggataca gagcattctc
      541 gttactcagc aaatgaccgc tttaccgatt taactgttcc gcgtgcgctg agccgcattc
      601 accgcatcac aaaattcact tttaaaaaag ggcggcagag cagttacgga gtaaaactga
      661 taccgccaaa tgtcaccaga atattgataa cagagggcgt tgcagcgggg ttgtcactta
      721 agcgtatggt caacctgaca acccggtgtc ctcaacgggg aaagaataac cccgccatac
      781 ttaccgccgc gccatttcgc ggattgccac aaccggaagc gcacggtcga attaaattta
      841 acgacggctc taagggagaa ggacttcgcc gtgcactttc gtgttatgcc ctgacttttc
      901 agggatatat cctttcagta aactgtcagt gccggattct tatctgtgtc cggcgcacga
      961 ccacacgtag cagcgtgttg gtctccattt ttaattcagc ccttaatgga ggataaaatg
     1021 ccaaacaaac catgccctgc ctgtaacgct ctttctggct ttttagagaa gggcggttat
     1081 tacattttta actgtccaga acatgttgaa tttcatatat caaaacttga cagtatcatt
     1141 acaaagccaa atcagtatca gtctgattta ctgaataaag agttaaacgc cgcccgagat
     1201 taagggcgta gtggcccatt aaaaatattg caaagaccaa ctattgttga actggattaa
     1261 accagtaatt ctctattgct cttattgtta ccatcggatg attgccgggg gttattttaa
     1321 gttcgggagg aaaaggctct ccatcagcag ccatcattga gtatgtgaaa tccgccgacg
     1381 cagtcccttt catgataatt ccgaattttt caagtaatga gagcagttcc ggtaatcctg
     1441 tgtcatctga ataactttcc tcatagaaag aagcgattgc cgcaggagat gacagttctc
     1501 gctctgcaat ttttttgtag ttttccagaa ttcttttttt gaatactttt cctcgacatg
     1561 aaaacccatc accgaggcaa tccgctttgc agtttcttca gtattctggt tgtttgttac
     1621 cgaaacagag tacaatccgg taaacgcatc gcgcacatta cgagccatat tatcaatgta
     1681 tttcttcgtt gcagattcta attcaacttc gttcagacgt tggtgaagtg catgtgctgc
     1741 aatttcctgg atttctttta gtaaatcttt aaattccatc tttaactccg ttagtcgtta
     1801 gattttgccg aactggaaag cgcctgttta aactcactga agctgagagc ttcctcgcct
     1861 tcggcaaggc cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc gaaatccata
     1921 tcactcacct gagtttcttt ccagccagcg acgggcacca ttttcggttt taaacgtttt
     1981 gcttttggta tacgtcatcg cggtgaatgt accgtcctgg ttgggaaaca cgccgtacac
     2041 cagagattcg ttgttgccaa gatcgatagt atccatgttg acctcatttc cccttaacgc
     2101 cgggtagcgg aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc
     2161 atacgcctcg ggctggctac ttaccccctg accactgctt ggtaactcga agtattgccc
     2221 ggcgttctgt ggggcggggt gggtggttgg tgtatgtaat ctacaataaa aaactgtttt
     2281 tgtgtcaaca gtttttaatt gttgttttgg gcaaaaaaat ccctcgaaag agggagtatg
     2341 aaaattgttc aactcagata gagaagggaa attgtcgccg agaatgtgtc acgcttacaa
     2401 tctcaatgct tgaagcagct actctgtaca ggattatgta gttagggtgg gtcacgatct
     2461 ctctcaatcc agaaacccgt tcgcttggtg gatataagta cggatgctca gatagaggta
     2521 ataccgatgt ttcaatgcgt atttttagtc tacgtgctgc cggtgggttc tccttggcga
     2581 tgtaagttat gatctggcgc aaatcatcgc gagcagacgg tagccataaa atgggtaaca
     2641 ttactcgctc ttgttagtta cagcaatttg agcaataaga ttttccattt cagccattac
     2701 ctcgtcatgt ggaattgcgg ggcgagtgtc tgcaaggctt gacgttactt tagtgcgcaa
     2761 ccattcgttg taactgtttt cttgttcggt agtttcgaat tctgaaacta tcggagaaag
     2821 ggctgtaccc atggcataac tcctcttctt gtactgtggt cacgcccggc ggcttttttg
     2881 tgccgccagc cacctagcaa tggtttcttc cattgatttt ttcttgtctt tgatttcttg
     2941 aagcattttt tcttggtctt cctcaggaaa cgcactaaaa gcttggagca gttcgcgttg
     3001 gcgaggacca attttcatcg tgtcaggggt gaaaatttgc tcacactctt caggaggcaa
     3061 taaaaaccaa tgcaatggat gccctgaaac ctcaaccagt ttatccaaac ttgaggcttt
     3121 aggtgtagcc ttaccgctga cccattgttg aacagtttgt tgtgtcacac caattctacg
     3181 ggcaagctca gcctggctcc atccagtttc ctgaagaagc ttgctgattc tgtacataga
     3241 tacttctagg gcgctcatca ttattcaatt ttacaggtaa atactgttaa aagcatcaca
     3301 ataaaaaact gttgattgca tacagttttt tattgtaggc tttgcttata gtttttagag
     3361 gagggcaaaa tgctagatag cactcgcgaa aaaattaggc agaaatacac tcaggctgaa
     3421 ataggtcgtt atatgggggt cgctcaacag actgtttggc aatggtttag ctttggcgtt
     3481 cccccaaagc aggtaattcc gttatgccaa ctaatgaagt gggaagttac cccgcatgaa
     3541 atccgcccag atatttatcc taacccaacc gacggtttac ctgttggatg taaggttaac
     3601 acatcaaatg cgccggagtt gattcatgaa aatcaagcat gaacacatcc gcatggcgat
     3661 gaatgcctgg gcgcatccgg acggcgaaaa agtaccggct gcgaaaatta ccaaagcgta
     3721 tttcgagctg ggaatgacgt tcccggaact gtatgacgac agccatccgg aagccatggc
     3781 tcgcaatact cagaaaattt tccgctgggt ggagaaagac actcctgatg cggttaaaaa
     3841 aattcaggcg ttgttaccag cgatcgaaaa agcgatgccg cctccgctgg tggcccgaat
     3901 gcgcagccac agttccgctt attttcggga gttggtagag acgaaggaac ggctggtgaa
     3961 agatattgat gatttcgttg catcagcgat cgttctgttc gatcagatga atcgtggtgg
     4021 cccggcagga aacactctgg ctgtgcatta attgggtaat aaatatgagt aatgacaaaa
     4081 aattgacact gagcgtttac gaaaacagtc cgcacatctg gcgtggcggt ttatctgatg
     4141 tggagctggc agagtggttg atacataaag ctaatgcgct gctctggcgt ttgtcagcca
     4201 gagaacagcg caaggaaacc agaataaagc tggctgatgc agaagcgtgt gccgggctta
     4261 ttgaggatta tacaaatctt ggtatttctt cagcagagag tgatcccatt cagcctctga
     4321 gcagggagtc aatccagcac gctggttgta tggcacatct tgtaactgct cgtcaacatg
     4381 aggtgggtat tggatcactt ccggcgggat attcgctgat tccagagctg gttgaagcaa
     4441 gaaaatcagt tcagaaaaag agagatgacg cacttcaatt attgagagag cactatggcg
     4501 cgataccaga atgcgaacag cgtcgatacc ctgaaggtta tgaatggatg cagtctcttt
     4561 ttgaagttcg ctaatcaata tgtcgagacg aaggtatgtt tcggcgcgca gccaggctct
     4621 gtaatccggg agcatttcgg ggctgttaca ccagcggttt gttgctgcaa catttaatac
     4681 atgagcctga taaaggcttt tcaaaaaata catgtcgaac ctcctctggt tctgtcgatt
     4741 gggaaccaca gattatatcc ggaggaaggt tcggcaccag atgaggtagc catgcgtgat
     4801 tacgcaaaag tttctccgcg attctggctg ggagaaacgg ggagagaact tagaaaggcg
     4861 ggtgcagaag cgcaagttgt tgctttttac ctgatgacat cccctcacgc aaatatgctg
     4921 ggtttgtatt acctgccagt tttatacctt gctcatgaaa ccgggcttgg tctggaaggg
     4981 gcttcaaagg ggcttaaaag ggctgttgaa gctggttttt gtagctatga ccatgatgca
     5041 gagatggtct gggtccatga aatggcagcc tggcaggttg gggaaacgtt gaagcctggc
     5101 gataaccgtt gtgcaggtgt caggaatgag tatgcatcat tacctgaaaa cgcttttctg
     5161 tcagtgtttt acgacagata taaaacggat ttccatctgg atgtgaggcg gaataatagc
     5221 cgaaattcgg taaggggctt cgaaggggct tttaaggggc ttcgaagcca agaacaggaa
     5281 caggaacagg agcaggagaa agaacaggaa caggacaaaa acactatggt tcatggcaaa
     5341 aaaaacacca cgaaccaggc aggggatgtt cagaccgtca atcctggtca gccagcaggc
     5401 acgacaccgg aagccgattc gggcgctgtg cagcaggtga tgaccgcagg gtcggagcaa
     5461 tcacaccaac tgcagcagcc tgaagccgat tccgccattc agcgggaagc cgatcgggta
     5521 gtcccggaaa gcaccgggca gtctgtggga cgagtggatt atccggatgt gttcgaacag
     5581 gtctggcggg aatacccgtt gcgtgctggg gcaaacccga agaaatccgc tttcagtgcc
     5641 tggaaggcca gattgcgcga gggggtgcca ccagagacca tgctggatgg tgtgaggcgt
     5701 tacgcgagat acctggcggc gaccgggaaa gcgggaacgg aatttgttca gcgagcgacg
     5761 acgttttttg ggccggaccg gaattttgaa aacccctggt tgctcccggt aagcggcacg
     5821 aacaaccagc gttgtgtgaa tcatatttct gaaccggata ccgaaattcc gccgggattc
     5881 agggggtgat gtggcatgaa aaacattgcg gcagccgggg ttcttgaacg tattcgcaga
     5941 cttgcaccac aggcgtcggt tccaccgtac cggacggtgg aggagtggcg ggaatggcaa
     6001 cttgctgaag gacgaaaacg cagcgaggag attaaccgcc agaatcacca gttgcgggtg
     6061 gaaaaaatcc tgaatcgttc gggcatccag cctctgcaca gcaaatgctc gtttgcgaat
     6121 tatcaggtgc agaacgacgg gctaaaatac gcgctgagcc aggccaaatc catagctgac
     6181 gaactgatga ccgggtgcac gaattttgtg ttcagcggta aaaccggcac cgggaaaaat
     6241 caccttgcag cggcgatggg taaccggctg atggcgaagg ggcgcagcgt gattatcgtc
     6301 accgtgtctg atgtcatgag cgtgttgcat gacagctacg acaacggcaa atccggggaa
     6361 aaatttttac aggagctttg cagtgttgat ttgctggtcc tggatgaaat aggcgttcag
     6421 cgggagacga aaaacgagca ggtggtatta caccagataa ttgatcgccg gacagcatca
     6481 ctgtgcagtg tcgggatgtt aacaaacctg aatcatgccg caatgagtac acttcttggt
     6541 gagaggatta tggaccgcat gaccatgaac ggtggtcgat gggtgacgtt taactgggat
     6601 agctggcgtc caaatgtcag caatatgagg gttgtgaagt aattttgtcc ggaggaaatt
     6661 ttaatggaaa ccgtatctga cgcactgaaa gcactgaaaa aagcctcttc acatgtggtg
     6721 gcagctcgcc ttggaatcag tcgtgaagag gctgtcaacg agctgtggga actcaaaata
     6781 aatggcgtcg ttgataaaac tggtcacacc tggtttctgg ctggcgaagg tgaatcccgg
     6841 gtaaccgaag agcggccagt aaaatctgaa gcacaggata tgctgaccgg ggaggtcgaa
     6901 caaaaagtta ccgcagacat gatgattgag tttatcggtc aggatggggc taaaacgtgt
     6961 gaggaactgg cgggtaagtt cggtgtcagt actcgcaagg ttgcttccac gctggcggtg
     7021 gtaaccgcaa cggggcggct ggcacgcgtt aatcagaacg gtaaatttcg ttactgcatg
     7081 ccgggcgata atttaccagc agagccgaaa gccgcgctgg taacggaaag tgatggtaag
     7141 gcctttcctc agccagcagg tgctgcgtta ccagtccggg aagccgcaac acaggaagaa
     7201 attaaaacag aaactgtggc ggacattgtg cagccgttgc catcgtttac cgaaacgcaa
     7261 gcagatgagc tgatttttcc gtcccttcgc agggcaaacc tggcgctgcg cagggcgaaa
     7321 agtgatgttc agaagtggga gcgagtctgc gccgcgctgc gggagctgaa caagcaccgg
     7381 gatattgttc gacagattac tgattcttcc cgccgtgttg tatcggaaaa gtgattgccg
     7441 gaggcgctta tggcaaaagt atttacacag gaagagcggg aaaaaattaa agggcaggtt
     7501 gttgagctag tacgccggag tgggcgcgag acgttacggc aactggaagc caagacaggt
     7561 gcgacaagat atctgatgag cgttcttgcc agagagctgg ttgccagtgg cgatgtatac
     7621 aactctggtt acgggttatt cccatctgaa caggctcgta aggactggca aaacgcccgc
     7681 aaaaaactat cgagggcaaa actgaagaaa ccggttgtgg ttgatcctga ccttatctgg
     7741 tcattaccag acggagaaat acgccgctat gacaggcaat tgaacataat ttgtagtgag
     7801 tgccgtaaca gtgaagtgat gcagcgagtt ttgatatttt acacaggagt aatgatggaa
     7861 tagtgaaaat aacatgaaac ctttggattc tggtgtttca gtggatggga aaagagcaga
     7921 tacatagaaa atgaatagca ataattcaca gtctggatgt tgtttgtatg cttataaaat
     7981 gatcctagta tgcattgccg gagttgaatc agctccaatg aagaaactga gaggagtatt
     8041 tagaagatag ctaaatggat gtaattatta gtatctaaaa aatagtattt ttgaaatggg
     8101 tctaagaaca caacaatgta caaatgagaa gttatctttt aatatgttca aagacccatt
     8161 aggattgata tcagttatgt tagc
//
LOCUS       DABSYT020000157         8180 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.158, whole genome shotgun sequence.
ACCESSION   DABSYT020000157 DABSYT020000000
VERSION     DABSYT020000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.158"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(327..812)
                     /gene="sixA"
                     /locus_tag="ID725_RS22780"
                     /old_locus_tag="pgaptmp_004556"
     CDS             complement(327..812)
                     /gene="sixA"
                     /locus_tag="ID725_RS22780"
                     /old_locus_tag="pgaptmp_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGV7853011.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(1015..3159)
                     /gene="fadJ"
                     /locus_tag="ID725_RS22785"
                     /old_locus_tag="pgaptmp_004557"
     CDS             complement(1015..3159)
                     /gene="fadJ"
                     /locus_tag="ID725_RS22785"
                     /old_locus_tag="pgaptmp_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGV7853012.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3159..4469)
                     /gene="fadI"
                     /locus_tag="ID725_RS22790"
                     /old_locus_tag="pgaptmp_004558"
     CDS             complement(3159..4469)
                     /gene="fadI"
                     /locus_tag="ID725_RS22790"
                     /old_locus_tag="pgaptmp_004558"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGV7853013.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4649..4933)
                     /locus_tag="ID725_RS22795"
                     /old_locus_tag="pgaptmp_004559"
     CDS             complement(4649..4933)
                     /locus_tag="ID725_RS22795"
                     /old_locus_tag="pgaptmp_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGV7853014.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5305..6645
                     /gene="fadL"
                     /locus_tag="ID725_RS22800"
                     /old_locus_tag="pgaptmp_004560"
     CDS             5305..6645
                     /gene="fadL"
                     /locus_tag="ID725_RS22800"
                     /old_locus_tag="pgaptmp_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGV7853015.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            7010..8041
                     /locus_tag="ID725_RS22805"
                     /old_locus_tag="pgaptmp_004561"
     CDS             7010..8041
                     /locus_tag="ID725_RS22805"
                     /old_locus_tag="pgaptmp_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853016.1"
                     /translation="MLSSISINNTSAAYPESINENNNDEVNGLVQEFKSLFNGKEGIS
                     TCIKHLLELIKNAIRGNDDPDRININNSSVTYINIGSNDTDHITIGIDNQEPIKLPAS
                     YKDKELVRTIINDNIVEKTHDDINNAEFTDGGIDDRSDIGIDSNDTDDIDIDNQEPIK
                     SPANDENKKVGQTIINENIVENTHDINNKKMIFSAIKEIYDEDPSFIFDKISHKLRHT
                     VTEFDESGKSEPTGLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDGYHIQKGFR
                     IDGDNLTQYCSKKARSASAWFEHSKAIIAESFATGSDHQVVNELKGERLRELNEAFNP
                     SNLARGYEY"
BASE COUNT         2245 a         2053 c         1837 g         2045 t
ORIGIN      
        1 ctgcgcaaaa aagacaatct tagattaagt ttgagattgt acttttttga agttatacta
       61 ttaacacatc acaaataata ttaaattaag acacgaaaat atcatttctt ttttattatt
      121 aactgttcta ttagtttgat gctttgtaaa aatacattac aacttagtct catttcgtca
      181 gaaattaata gggaagcaga ggaaaaagtg cgatgaattc tgtatcgaag cgtatcaact
      241 ccgtgaaggg ttttgagtga atttctacta ttgcaaaaag acaaatgccc gatgtacagc
      301 ttacatcggg cattttttca aacgaatcag atagctttcg ccatcttcaa attacatggg
      361 ctcatctgcc agttgaactc gccttttccg ctttcatcaa gcgttacgct ggcaatggcc
      421 gaggtggtaa acataggcgg cgtttcgccc ggacataact cggcgaccag atacccaact
      481 aacggtaagt gggagataac caacaccgaa gcaacacctt cattggtcaa cgcctgcaaa
      541 taggcactga ccagaccaac atcgccgcag ggcgttaact ccggcagaac ttctgcactg
      601 gaaggcaggt tcagacaatc cccaacttcc tccagtgttt gctcggctcg caggaacggg
      661 ctcaccagaa cgcgttcgat ttccactttt tgacctttca gccagttcgc catcaggcga
      721 gattcgtcac aaccgttagt ggtcagagga cgaaccgaat cactggcggc atcgagggct
      781 gcgtcgccgt gacgcatgat aaaaacttgc atattgcacc gcttttgtta accagattcg
      841 cccgttttct ttcatctgaa accagagaaa gaaaacggtg gccggcattg tgccttatcc
      901 attcaccgaa tgaaacgctg ttttttacct caatggcgta agtatagtca atatgcgttt
      961 acattttgac caacaatccg ccattcagcg cggattcata tagctttgac cttcttattg
     1021 caggtcagtt gcagttgttt tccaaaaact ttccccacgc gcacccatct cgactaaacg
     1081 ctcgcaaggg gtaaaacgtg aaccatactg cgtggccagt cgttgcatta ttgcaaccac
     1141 ttcgcccgcg ccgagagaat cgatatagcg aaacggtcca ccgagaaatg gcggaaaacc
     1201 aatgccaaat accgcaccaa tatccccgtc acgcacgcta cggataacct gctcatcaac
     1261 acaacgtaca gcttcattca gcatcaacat cacacaccgt tcagcaacct gcggtgcgga
     1321 gagtcgcccc tgcccttgtg cgccaatgag cgggtaaatg gcaggatcga cctgtttttt
     1381 gcttttacgc cctttctgac cataaagata gaaacctcga ccattttttc tgcctttgcg
     1441 atcgtcgttc aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt ctccataagc
     1501 ggcttccagt acaggaataa ttttagtccc ggtgtcgatt cctacctcat ccaaaagttg
     1561 gattggacct accggaaaac caaatttcac tagcgcggca tcaatgtgcg ctacccgttc
     1621 accttcggtc agcatgcgga tagcttcatt aatgtaaggc gctaagatgc gattgacgta
     1681 aaacccggct ttgtcacgca cgacaatggg cgttttgccc tgttttttcg ccagttttac
     1741 cgtagtggcg atggtttgtg ctgatgtacc cgcatgagga ataatctcca ccagcggcat
     1801 tttttccacc ggactgaaga aatgcaggcc gataacttgc tcaggtcgcg tggcgtgagc
     1861 ggcgatatca ccaatcggta aagatgacgt attcgaagca aagatggtat gagcggcgca
     1921 attttgctca acttccgcca ccatctgttg tttcaattcg agattttcaa acaccgcttc
     1981 aataatcaga tcgcgatggg caaagccgcg atagtccgtc gttccggaga ttaatgccag
     2041 ctgtttgtca cgttcgctgg ctttgagatg acgacggcga actttgccct ccagctgatc
     2101 ccaactgtac ttcagcgcat gatttatgcc ctgcgggttg atatctttaa ttctgaccgg
     2161 aatccccgct ttacaagcag tgacataagc aataccgccg cccatcaagc caccacctaa
     2221 aatccccacg ctgtttaatg gcgcaggcgg cgcatcactg ccgggatctt tcttcacttc
     2281 cgtactggca aaaaagatac tacgcaaagc ttgcgattgt ggcgtcatcg ccagttcgcc
     2341 aaacgcccgt gcttcagcgt catagccact gctggtgccc tgcgctaatc ccgtttcaac
     2401 aacctccagg atgcgttctg tcgccggata attgccttga gttttgtgtt ctgttttctt
     2461 gccgaccatt ttgaacagca gcgcacgacc taacggcccc gccagaatac gctcgcgtac
     2521 aggtagaggg cgggaagatg ggcgatcctt ctttgccagc tcaacagcgg cttccagcag
     2581 aatggagtgc ggaacaacat catccaccag ccccagcttt aatgcctgtt tcgcccgaag
     2641 ttgttttccg gtgaggatca tctctaatgc tgtgctgacg cctatcagac gcggtaaacg
     2701 ctgggtgccg cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac cgagcaccgt
     2761 tttaggatcg tcagtacaaa cgcgaccgtg gcacgccagc gccaactcca gcccaccacc
     2821 caggcaagcg ccatgaatag ccgcgataac cggaatgggc aaagcatgaa tctccgccat
     2881 caactgttgg ccctgccgcg ccagagcttc cgcttcttgc gccgttttgc aattgccgat
     2941 catgttgatg tctgcgccag caatgaagtt gtccggttta gcggagacaa acaccacgcc
     3001 tcgcaactct ttgttttcac ggagttgctt aataatggcg cgcacctgcg aggcaaactc
     3061 cgccttcagg gtattcattt tctcacccgg tacgtcgatg gtgatgatag caatgttgtc
     3121 cagacgaaca ttaagagtaa acgctgatgt catttccatt attccgcctc cagaaccatt
     3181 gccgcgccaa gcccaccggc agcacaggcg gtaactaaac caaatccacc gccgcgacgg
     3241 cgaagttcat gcaatgtctg ggtaatcatc cgcgcgccgg tcgccgcgaa gggatgcccg
     3301 taagcaatcg aaccgccaag cacgttaaat ttgctatcgt ccacttcgcc agtggcatgt
     3361 gcacgcccca gcgcttcacg agcaaaacgt tcactaccca gtagctgaat attggccaac
     3421 gtctgggcgg caaaggcttc gtgcatatca atcaatgtca gatctgccat tgtcaaaccg
     3481 gcacgctcca gcgccagcgg tgttgaccag gctggaccga gcaacatgtc ctgccagaca
     3541 tcgatagcgg taaatgcgta gctgcgcaga tatcccagcg gtaccagccc taattctttc
     3601 gcccgggatt cagtcatcag gatcaccgcc gctgcgccat cggtcagcgg cgtactgttc
     3661 gccgccgtta ccgttccgtg tttacgatca aacgccggac gcagctttgc gaaatcagca
     3721 agcgaggaat taccgcgaat attgttgtct tccgcgagca gttgtttata aggagggata
     3781 aaggcagtca tcacctcttc tttgagtttt ccgtctgacc atgcctgagc ggcacgctga
     3841 tgcgaacggt gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt tttcgccatt
     3901 tgctctgcag tgtcgcccat ccgcaagccg gtagaatatt ctgctaccgc aggtggtacg
     3961 ggcattaagt cgcgcaaacg caggcgagag aagagtttca gacgctggct cattgtgcgg
     4021 gctttgttga catcaaccag cacgcgcgcc agttttttac tgacgccaat tggcaatacc
     4081 gaagaggaat ctgccccacc ggcaatcccc gctcggatag tccccgccat caggctttct
     4141 acgacgtttg caaccgcctg gaaactggta gcgcaagcgc ggctgacgct gtaagcatcg
     4201 gtatgcacat tcattccagt accgagaaca atttcacgcg caatgttggg agcttcaggc
     4261 atttgtacga cctgaccaaa gaccagttgt tcaatcactt cggcggggat ctcgctgcgt
     4321 gccagcagtt cgcctaccac catcttccct aaatcaaccg cgggaatgcc atgaaaagcc
     4381 gtcgcctggc gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg atcgccctgg
     4441 cgggtaacca gcggtaaaac ctgacccata acactcccct gtaaaaaaat aaataaagtg
     4501 gtctgacctg atcatagtct taaccatttt tttacattta gccaagcgga gaaaagggaa
     4561 attgggagct atgacacagt gaagacagga gaaagaaaag aaaacgcccc tacctgttgc
     4621 tggcaggagc gtacttatcc gagaggaatt aacgcagacc cagctggaag atcagcattt
     4681 cagcttcgca ggcaaaagtg aaatcgatat ccaggcgcac accatcagac tcttcagtga
     4741 aagtcgggga gattttgcac ggttcggatt ccacgctacg ggctttctca gtcagcgccg
     4801 ccagcgtttg ttctgcttct gcgcggtttg caaacacgcg gctgtaagac gctgtgcagt
     4861 cggagttgtc cataatggtg ccaacatcca tacagcagca aaccggggtt tcatcagcac
     4921 tacatttact catcgttgat ttcctctgta tgtgcaccca aggtgccaga taaacgttgt
     4981 ggatatttta cgcttccgga aagtgctgct ccagttgtta attctgcaaa atcggataag
     5041 tgaccgaaat cacacttaaa aatgatctaa aacaaaattc acccgaatcc atgagtgcgc
     5101 cacctccaaa ttttgccagc tggatcgcgt ttcttagatc atatttgaaa aaagatagaa
     5161 acatacttgc aacattccag ctggtccgac ctatactctc gccactggtc tgatttctaa
     5221 gatgtacctc agaccctaca cttcgcgctc ctgttacagc acgtaacata gtttgtataa
     5281 aaataaatca ttgaggttat ggtcatgagc cagaaaaccc tgtttacaaa gtctgctctc
     5341 gcagtcgcag tggcacttat ctccacccag gcctggtcgg caggctttca gttaaacgaa
     5401 ttttcttcct ctggcctggg ccgggcttat tcaggggaag gcgcaattgc cgatgatgca
     5461 ggtaacgtca gccgtaaccc cgcattgatt accatgtttg accgcccgac attttctgcg
     5521 ggtgcggttt atattgaccc ggatgtaaat atcagcggaa cgtctccatc tggtcgtagc
     5581 ctgaaagccg ataacatcgc gcctacggca tgggttccga acatgcactt tgttgcaccg
     5641 attaacgacc aatttggttg gggcgcttct attacctcta actatggcct ggcaacagag
     5701 tttaacgata cttatgcagg cggctctgtc gggggtacaa ccgaccttga aaccatgaac
     5761 ctgaacttaa gcggtgcgta tcgcttaaat aatgcatgga gctttggtct tggtttcaac
     5821 gccgtctacg ctcgcgcgaa aattgaacgt ttcgcaggcg atctggggca gctggttgct
     5881 ggtcagatta tgcaatctcc tgccgggaag actcctcaag ggcaagcatt ggcagctacc
     5941 gccaacggta tcgacagtaa taccaaaatc gctcatctga acggcaacca gtggggcttt
     6001 ggatggaacg ccggtatcct gtatgaactg gataaaaata accgctatgc actgacctac
     6061 cgttctgaag tgaaaattga cttcaaaggt aactacagca gcgatcttaa tcgtgtgttt
     6121 aataactacg gtttgccaat tcctaccgcc acaggtggcg caacgcaatc gggttatctg
     6181 acgctgaacc tgcctgaaat gtgggaagtg tcgggttata accgtgttga tccgcagtgg
     6241 gcgattcact atagcctggc ttacaccagc tggagtcagt tccagcagct gaaagcgacc
     6301 tcaaccagtg gcgacacgct gttccagaaa catgaaggct ttaaagatgc ttaccgcatc
     6361 gcgttgggta ccacttatta ctacgatgat aactggacct tccgtaccgg tatcgccttt
     6421 gatgacagcc cagttccggc acagaatcgt tctatctcca ttccggacca ggaccgtttc
     6481 tggctgagtg caggtacgac ttacgcgttt aataaagatg cttcagtcga cgttggtgtt
     6541 tcttatatgc acggtcagag cgtgaaaatt aacgaaggcc cataccagtt cgagtctgaa
     6601 ggtaaagcct ggctgttcgg tactaacttt aactacgcgt tctgataacg cgttcgcctg
     6661 gataaagtca cctgctatgc aggtgacttt aactccccca cttcaccgaa agtagtttcc
     6721 tcattgcttg cctcctgagt tttgcaaaca gcctgatggc agctagccct ttcaaatcag
     6781 taagtggtat ccacagacaa acctgaaggg aaacgcatat tttcaggcgt tctgctcgtc
     6841 cttctcaaag agtttacttt tctgaatttc caggatactc aaccccctgg ctattgtgcg
     6901 ctcatacacg caaattaaag ataggttcta aataaatgag cgttttttga tagtctattt
     6961 cattaggtaa tatatatttg taacaaatca atcaacatgg aataaaatca tgctatcatc
     7021 tatttcaatc aacaatacca gcgcagctta cccagaatcc atcaatgaaa ataacaatga
     7081 tgaagttaat ggattagtac aagagttcaa aagccttttt aatggtaagg aaggaataag
     7141 cacctgtatt aaacatctac ttgagcttat aaaaaacgcc atacgaggaa acgacgatcc
     7201 tgatagaata aatatcaata attcttcagt tacttatatt aatattggtt ccaatgatac
     7261 agaccatatt actattggta tcgacaacca agaaccaata aaattacctg cgagttataa
     7321 agacaaagaa ctcgtccgta ctatcattaa tgacaacata gttgagaaga ctcatgatga
     7381 tataaataat gctgaattta ctgatggtgg tattgatgat cgtagtgata ttggcattga
     7441 ctccaatgat acagacgata ttgatattga taatcaagaa ccaataaaat cacccgcgaa
     7501 cgatgaaaac aaaaaagtcg gccaaaccat catcaatgaa aacatagtag agaatactca
     7561 tgatatcaat aacaagaaaa tgatcttcag cgcaataaaa gaaatatatg atgaagatcc
     7621 aagttttatc ttcgataaaa tatcacacaa actcagacat acggtaacgg aattcgatga
     7681 gagcgggaaa agcgaaccaa cgggcttatt tacctggtac ggtaaagata aaaaaggcga
     7741 ctctctcgct attgtaatta aaaataaaaa tggaaatgat tacttatctc tcggttacta
     7801 cgatcaggat ggctaccaca ttcaaaaagg atttcgtatt gatggtgata atctcaccca
     7861 atattgtagt aaaaaagcca ggagtgcttc tgcgtggttt gaacacagta aagcaatcat
     7921 tgcagaatca tttgcaacag gttccgatca tcaagttgta aacgaactca aaggggaaag
     7981 actgagagaa cttaacgaag cttttaatcc ttcaaatcta gcaagaggat atgaatatta
     8041 agaggatgat agaaaatata catttgagaa tacaaaagaa atagtttcta ctttagtcgg
     8101 taacaaagtt aacgttggcc attctcaaaa aatatataaa cattttaagg atctcgaagg
     8161 taaaactgaa gaaaggcttc
//
LOCUS       DABSYT020000158         7929 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.159, whole genome shotgun sequence.
ACCESSION   DABSYT020000158 DABSYT020000000
VERSION     DABSYT020000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.159"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(102..1589)
                     /gene="yeeO"
                     /locus_tag="ID725_RS22810"
                     /old_locus_tag="pgaptmp_004562"
     CDS             complement(102..1589)
                     /gene="yeeO"
                     /locus_tag="ID725_RS22810"
                     /old_locus_tag="pgaptmp_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGV7853017.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            1750..1825
                     /locus_tag="ID725_RS22815"
                     /old_locus_tag="pgaptmp_004563"
     tRNA            1750..1825
                     /locus_tag="ID725_RS22815"
                     /old_locus_tag="pgaptmp_004563"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1783..1785,aa:Asn,seq:gtt)
     gene            complement(1863..2813)
                     /gene="cbl"
                     /locus_tag="ID725_RS22820"
                     /old_locus_tag="pgaptmp_004564"
     CDS             complement(1863..2813)
                     /gene="cbl"
                     /locus_tag="ID725_RS22820"
                     /old_locus_tag="pgaptmp_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGV7853018.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2915..3832)
                     /gene="nac"
                     /locus_tag="ID725_RS22825"
                     /old_locus_tag="pgaptmp_004565"
     CDS             complement(2915..3832)
                     /gene="nac"
                     /locus_tag="ID725_RS22825"
                     /old_locus_tag="pgaptmp_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGV7853019.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4158..4233
                     /locus_tag="ID725_RS22830"
                     /old_locus_tag="pgaptmp_004566"
     tRNA            4158..4233
                     /locus_tag="ID725_RS22830"
                     /old_locus_tag="pgaptmp_004566"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4191..4193,aa:Asn,seq:gtt)
     gene            complement(4289..5224)
                     /gene="ldtA"
                     /locus_tag="ID725_RS22835"
                     /old_locus_tag="pgaptmp_004567"
     CDS             complement(4289..5224)
                     /gene="ldtA"
                     /locus_tag="ID725_RS22835"
                     /old_locus_tag="pgaptmp_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGV7853020.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5286..6365)
                     /gene="cobT"
                     /locus_tag="ID725_RS22840"
                     /old_locus_tag="pgaptmp_004568"
     CDS             complement(5286..6365)
                     /gene="cobT"
                     /locus_tag="ID725_RS22840"
                     /old_locus_tag="pgaptmp_004568"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGV7853021.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(6377..7120)
                     /gene="cobS"
                     /locus_tag="ID725_RS22845"
                     /old_locus_tag="pgaptmp_004569"
     CDS             complement(6377..7120)
                     /gene="cobS"
                     /locus_tag="ID725_RS22845"
                     /old_locus_tag="pgaptmp_004569"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGV7853022.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7117..7662)
                     /gene="cobU"
                     /locus_tag="ID725_RS22850"
                     /old_locus_tag="pgaptmp_004570"
     CDS             complement(7117..7662)
                     /gene="cobU"
                     /locus_tag="ID725_RS22850"
                     /old_locus_tag="pgaptmp_004570"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGV7853023.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7814..>7929)
                     /locus_tag="ID725_RS22855"
                     /old_locus_tag="pgaptmp_004571"
     CDS             complement(7814..>7929)
                     /locus_tag="ID725_RS22855"
                     /old_locus_tag="pgaptmp_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HGV7853024.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         2186 a         2158 c         1719 g         1866 t
ORIGIN      
        1 aatcgtgaga acgaggcgaa tattagcgat gcccacccac aatgtcaaag cctgtttttt
       61 aaatttgaaa tcgtttgctg aaataatctg cattttgtcg tttattccga cacagctggc
      121 tttttttcac acttttgcgg ttcgggtcga gggtatttcc acagccaacg tccagtaacc
      181 attcgccagt aaaacagcac ggcccgcaca gcccagtcgg caaacattcc catccagaca
      241 ccaaccacac cccagccaag catgatgccc agcacataac cgacgacaac ccgacaaccc
      301 cacatgctca acatagaaac ccacatagcg taacgggcgt cacgagcccc cttaaatcca
      361 gcgggtagca cccatgaagc ggaccaaata ggcataaata aagcatttag ccaaatcaga
      421 atcacaacga catgtttaac ctgtggatcc tgggtgtaaa acgatgccat aaccccggca
      481 aagggagccg ttagccaggc gatggccgtt aatccaagag tggtcagcca aaacacatgc
      541 cgcaactgaa tctctgcttg cgctatctgc cctaccccca accttcggcc tgtaatgatc
      601 gtagaagcag aaccgagcgc acttccgggt aagttgataa gagccgcaat tgaaaacgcg
      661 ataaaatttc cggcaataac actggtcccc attccggcaa cgaacatttg ggttaataac
      721 cgaccactgg taaataacac tgattcgaca ctcgctggaa taccaatccc catgacttcc
      781 cagataatgc taaaattcag cggtttaaaa tagctcttta acgaaatcct tagcgcagga
      841 ttaaaaccaa tcgccagcac ccacaaaatt gcaactgcgc caatataacg agaaatggtt
      901 aaacccaacc cagccccgac aaatcccagt cccggccagg agaaaaggcc gtaaatcaat
      961 atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag cggtattttc
     1021 gtattccctg caccacgaag tgccccgcta ccaattagag tgatggcagc tgctggataa
     1081 ctgagtaccg tcagctccag ataagtcaac gccagtgctt taacttctgt cgtggcatca
     1141 cccgcgacga aatcaataat ttgctcgcca aaatgatgaa taagcgttgc caacagtacg
     1201 gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg tcgtcgatcc
     1261 cgcttaccga gactaaatgc cacaacgaca gtagtaccaa gatcgatagc agcaaaaaaa
     1321 gccataatga ccatattgaa gctgtccgcc aatcccacgc cggccatcgc atcttttccc
     1381 agccagctga ccagaaaagt gctcagaacc cccatcaaca ggacacaggc attctccatg
     1441 aagataggaa cagcaaacgg ggttatctcg cgccagaaca acactttgta gctcttgcgt
     1501 ttagcgtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat attcaaagcc
     1561 gaccttaatt gcagaaagtg aaaccacatt tcaaataatg agggagaatc agcaaagctg
     1621 caaagatttt cgccatcaaa ttgtctgcaa atgcaacaaa ctgttgatag aaacggcaaa
     1681 cagttgggaa aattaaaaat cgggtttgac aaaagatttt tcgccgttaa gatgtgcctc
     1741 aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact
     1801 ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata gcgggctttt
     1861 tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctgccg cttgatatcc
     1921 tcaactgaaa gtcctgcatt gcaaagttcc agaaagcgcc agacatagtt acgctgaagt
     1981 tgtcctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg cgtatccagg
     2041 cggattaaat tcttttcctc ttgttcaccg ctggattgtt cggcaactaa tccgatccca
     2101 agcccaagag caacataggt tttaatgaca tcagaatcct gcgcacttaa tataatatct
     2161 gccagcaaac ctttgcgggc aaatgcgtca tcaatacgtg agcgccccgt aatcccctgt
     2221 cggtaagtga ttaacggcca cttcgctatt gattccagcg tcaatggtgt aatttgcgtc
     2281 aagggatgat cgtgtggaac aagcaaacta tggtgccaac gaaaccacgg gaaggcgacg
     2341 agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc accattatgc
     2401 aacaacgtcg caatttcctg tggcgtcccc tggattagct cgagccgaac ctccgggaaa
     2461 agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt atgcgtcgtt
     2521 gcaatagtga gaacgccaga cgtatcgttg gtaaacaggt ctgcaagccg acgaacatta
     2581 ctggcttcat tgagaatacg ttctgcaatg accagtaatg ctttgcccgg ttcagtcatg
     2641 cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caagttcatc ctccagttcc
     2701 cgaatatgac ggctgacgcc tgactgtgag gtaaaaagca tattcgcaac ctctgtcagg
     2761 ttgtaatcct gacgtgcagc ttcgcggatt atctttagtt gttggaaatt cacggtaaac
     2821 tccgggcagt tcagatatcc cgttattgtt aaagtctaac gcccggcata acaaataata
     2881 aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc cactgccttt
     2941 tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc tgtgcctgtg
     3001 gcgataagtt cgctctggcg ggtaaattta atgacaaaga gagactcatg gaaggcgtgg
     3061 taatgcgtga catccaccca tttactgcgc cacataacga acgcgcggcc gattcgggta
     3121 atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtagcaata gactcaattt
     3181 caccaatgac ttttgccgtg agccgccgta gggaaaacgc ctcatcaaca cgaagtctaa
     3241 cagcgctgta atcactgggg agaaaaaggt tcatttgtgc aatagcatta acatcaacgc
     3301 tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc aaagcctgac
     3361 tggatacacc agccacaggg gaatgctcat aaatcaccgc catatcgagt tggtgattta
     3421 tcagtttttc gttaagcact gcaccgctat tttcatgaag atagataacg acctccggga
     3481 attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg gttcctggcg
     3541 caaagccaat cgagacttgc cccgataatg cctgaccaac gttatgcacc gccagttggg
     3601 cctgctcaca ctgacgtaaa atggcccgcg catgggtata gagaattttt ccggcgtctg
     3661 ttggcgtaac gccccgtttt gtgcgaatca aaagttgttg atttaactca ccttcgagtg
     3721 tggcaacctg ctggctgagc gctggttgtg cgatatgcaa tacttcagca gcctgagtca
     3781 ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc atgttgcctc
     3841 cggtttttga gaatcggccc aagtgccgcc attacttaca accagattgc aagatgcttg
     3901 ccagttttat tttggcgttg atgtacaagc taaccaactg tcaaataaga gattatgata
     3961 gattcgtcat tggctctttt caccagcagt cgcgttcccc tgccctataa aaggagggta
     4021 tgcaccacga tggttcatta ccaataagat tgaaatctca tcactttgtt gaaattgaca
     4081 gcaaacaaac aaaaaaatgc atttcaccct ttgacatcac catgcactgc cattaatatg
     4141 cgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     4201 atgtcactgg ttcgagtcca gtcagaggag ccaaattcaa aaaagcctgc tttctagcag
     4261 gctttttgct ttctaattac caacgctctt aaaacatctg tcttgaacca gaactaattt
     4321 gcacaggcat tcccgatcga cgttgcaacg cagcatttgc gcgattcaca tcaacttctt
     4381 gcccgttgat aaacgcccgc aaagatgggg ttaccggcaa tggcactttt cggtcagact
     4441 catattcagc acgattgcgc gacagcggct catgaacttc cagccacttt gaaccatccg
     4501 gttccgttgt gtatttcact ggctgatcaa taatttgcac acgagtacct acaggaacat
     4561 tatcaaacag atatttgata tcgtcattgc gcagacgaat acagccctga cttacccgga
     4621 gcccaatacc aaaattggca ttggtaccgt ggatggcata tagcctgcca atataaatcg
     4681 cgtacagccc catgggatta tcgggccccg caggaacaaa tgcgggcaaa ctctcccctc
     4741 gtttcgcata ttcgcgccgg gtgttcggcg ttggcgtcca ggttggtgct tcttgtttac
     4801 gttcaacggt agtcacccag ttacgcgggg tttctcgccc agcctggccg ataccaatag
     4861 gaaagacttc cacagtatta ctgtctggtg ggtagtaata aagacgcatc tcagcgacgt
     4921 taaccacaat ccctttacga acagtgacgg gcaaaatcag ttgctgcgga atggtgagtt
     4981 gcgaacctga cttcggtaag aagacatcag cgcccggatt cgcttccagc atgttactta
     5041 acccttgccc gtattgtgcg gcaaaagtct ccagcggctg ggtattgtga tcaggaacag
     5101 ttacagtaag cgactgcccc actaaacggc taccctctgg aggtaatgga taagttaccg
     5161 ccaggctagt atggctggca aaaagcagag caaatgagca aagaatattt acacgacgca
     5221 tcatgtccct ttcctaagtc gcgaaagcta tcggttaagt atagctttta tcagactttt
     5281 cgtttttaac tgttcaaatc agaagtcgta ttccccggta gaacaatttt actggcagca
     5341 agttcgccca tgttgttgta tatcgcacaa gcagcttcga tgatgggcat cgccagagct
     5401 gcgccactcc cctcacctaa acgcatatcc atattgagaa aaggctccag ccccaaatgc
     5461 gacagcgcta tacgcgcccc tttttctgcc gacaggtgag aaggaatgag atacggtttg
     5521 attgcaggag acatctggca ggctgcgagc gcagcagcat aagaaagaaa tccatccagc
     5581 aacacgggta aaccgcagga agcagcgcct aacatcacgc cagccattcc gaccaaatca
     5641 aatccaccca cttttgccag aacattaacg ccatcctgcg gatttggctg attcaacgta
     5701 atcgcccggc gcacaacatc aattttatta gccagtttat ctgtcggcag gtttgcgcca
     5761 atcccaacca cttcttcagg atcccggcca gtgattgtgc tgactattgc tgctgccggt
     5821 gtcgtgtttg ccatccccag ttcacctaca ccaaacagcg tgacaccgtt ttttgccagc
     5881 tcccgcgtat aacatatgac gtccaaaagt aacttttcag cctgacggcg actcattgcc
     5941 ggagctgaag caatattgcc gctacctcgt gcgacacgca tgttgataag cccggggata
     6001 ggctcagcag tatcaatacc aacatcaatt acgtggacgt tagcgcccgc ttgtgctgcc
     6061 agcacacaca cgccggttgt tccgcgggtc atattttcag cctgaatggc tgtcacttct
     6121 tttggggaaa tagcgacccc ttcctcccag acgccgtgat cggcacacat aaccagtacc
     6181 gcttttttgc ccacatgcgg tatgccattc aaccccggca ttcccgccag ttgtacgcca
     6241 agcgcctcca gccttcccag gctaccaaca ggtttgagta acccgtcaat atgccgttgt
     6301 gcacgcgaca tagcggcagg atcgatggca gggatcgtat tcagtaaatt ggcaagtgtt
     6361 tgcatgtcac gtctctttat agcagtgcca gcaggaagac cagttcacca agctctatgg
     6421 ctgctcccag cgtatcgccg gtttgcccgc ccagcgtacg tttgagaagc tgaccgagga
     6481 tgaaaatcgc caccatcgtc accaccatag cagccacacc atgcataccc ggcaataata
     6541 cggcagcaaa aatagcggct aagccgagtg tgacgcaggt ttgtcgccca tcaattttgc
     6601 caatgaacac attgccaagc ccttcttccc gggcataacg atgacgatac atcaataatg
     6661 cggcagtccc gcggctgacc gcacatgctg ccgccagcga tgcaaggatc ggttcgccac
     6721 gcagagccaa ctcgcttaac accaaaatct ttgccagtac aacaaaaatc aatgccagac
     6781 cgccgtgggt acctaaacgg ctatcacgca tgatttccaa catgcgatcg cggctacgtg
     6841 cagaaaatac gccatcacag gtatcagcga ggccgtccag atggaatccc ccggtcatca
     6901 gcaccagcac aagtacgcta aacagtgccg ccagtggtgc gccacaccat gcctgcagca
     6961 ccatgaagac cagcccgcta atcgcgccaa gcaataatcc aatcaaagga aaagtaataa
     7021 taccgcgaga ataatgctcg aaatccagtc cctgggacca gcgacgcggt acaggcagac
     7081 gcgtaataaa tgagagcatc gcccaaaata atttactcat ttgattttta ctccaatacc
     7141 cgaaaccacc agccatactt catttgccgc agcggccaac tgctgattta cccgcccggc
     7201 aatatcacga aaatgtcgtg ccagacgact ctccggcaca atccccattc ccacttcgtt
     7261 agtcactaat acaacctttg cggggcaacg ttggcaggca gcaatcaacg actgaatctc
     7321 agcattaatc gcctgttcca tcgcctgata atcccattca tcagggtctt tatcgccgcc
     7381 ataatcaaac aacagattag tcaccattgt ggtaacgcat tcaagcaaca caacctcatt
     7441 cgggttaatg tctgcatgaa ttaattcatc aagatgttgc cagcgctcca ctgtgcgcca
     7501 gtgctccggg cggccttgcc gatgatgttc tatccgtgca gccatctcat catcaaggat
     7561 ttgcgaggta gcgatataca gaacctgtga agagtcccca ataagcgcct ctgcgtggcg
     7621 actcttcccg ctccgtgcgc cccccgtcac caaaatcatc ataccgactc ctgattcaaa
     7681 tgtatgatgg tattcattga acagaacaat ggatccccca ccgttgctga ccgcaaaaca
     7741 tttacgcgat taatactgcg cgtaatataa ttaaaaatcc agcattctca atccattacg
     7801 aaataagatg gcattacgga atagccagac tttctcgtgt taacgtccga caaaatgcgt
     7861 cattgacagc ggtttctgtg gattgacaca tttcattcag ataatgaatt aatgctactg
     7921 ccggaacaa
//
LOCUS       DABSYT020000159         7813 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.160, whole genome shotgun sequence.
ACCESSION   DABSYT020000159 DABSYT020000000
VERSION     DABSYT020000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.160"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            145..873
                     /gene="gadW"
                     /locus_tag="ID725_RS22860"
                     /old_locus_tag="pgaptmp_004572"
     CDS             145..873
                     /gene="gadW"
                     /locus_tag="ID725_RS22860"
                     /old_locus_tag="pgaptmp_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGV7853025.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1236..4349)
                     /gene="mdtF"
                     /locus_tag="ID725_RS22865"
                     /old_locus_tag="pgaptmp_004573"
     CDS             complement(1236..4349)
                     /gene="mdtF"
                     /locus_tag="ID725_RS22865"
                     /old_locus_tag="pgaptmp_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGV7853026.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4374..5483)
                     /gene="mdtE"
                     /locus_tag="ID725_RS22870"
                     /old_locus_tag="pgaptmp_004574"
     CDS             complement(4374..5483)
                     /gene="mdtE"
                     /locus_tag="ID725_RS22870"
                     /old_locus_tag="pgaptmp_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGV7853027.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5591..5869
                     /locus_tag="ID725_RS22875"
                     /old_locus_tag="pgaptmp_004575"
     CDS             5591..5869
                     /locus_tag="ID725_RS22875"
                     /old_locus_tag="pgaptmp_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853028.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5870..6397)
                     /gene="gadE"
                     /locus_tag="ID725_RS22880"
                     /old_locus_tag="pgaptmp_004576"
     CDS             complement(5870..6397)
                     /gene="gadE"
                     /locus_tag="ID725_RS22880"
                     /old_locus_tag="pgaptmp_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGV7853029.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7196..7768)
                     /gene="hdeD"
                     /locus_tag="ID725_RS22885"
                     /old_locus_tag="pgaptmp_004577"
     CDS             complement(7196..7768)
                     /gene="hdeD"
                     /locus_tag="ID725_RS22885"
                     /old_locus_tag="pgaptmp_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGV7853030.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2072 a         1871 c         1833 g         2037 t
ORIGIN      
        1 caattaactg ttatgcaaaa ctactttgtg gataaatttt ggtcctacca aatttggcag
       61 tttttgcgct aagaaacagt ctggcatcat ttcattagta tactgaaatt gaaataatcg
      121 cagtatgaaa tataagggat aatcatgact catgtctgct cggtgatcct cattcgtcgt
      181 tcattcgata tttatcatga acagcaaaaa atatcgctgc ataacgagag tatcctgctg
      241 ctggagaaaa atttggcaga cgattttgcg ttttgttcac cggatacgcg acgactggat
      301 atcgatgagc tgacagtttg ccattactta caaaatattc gtcagctacc acgcaattta
      361 gggttacata gcaaagaccg tctgttaatt aaccagtcac cccccatgcc gctggtgacg
      421 gcgatttttg atagcttcaa tgaatccggg gtaaattcac cgatactgag caatatgctc
      481 tacctttcct gtttatcgat gttttctcat aagaaagaac tgatcccctt acttttcaat
      541 agcatcagca ctgtttcagg aaaagttgaa cgccttatta gctttgatat cgccaaacgt
      601 tggtatctgc gcgatatcgc ggaaagaatg tataccagcg agagtctcat caaaaaaaag
      661 ttgcaggatg aaaacacctg tttcagtaaa atattactcg cctccaggat gtcgatggcc
      721 agacgattac tcgagttacg tcaaattcct ctgcatacta ttgcggaaaa atgtggctat
      781 agcagtacgt cgtactttat aaacacattt cgacaatatt atggtgtaac gccacatcag
      841 tttgcgcaac attcgccagg taccttttcc tgacatattt tgcatttgaa tattggtcag
      901 gatctcacac ctgcttcatg tgaaactctt ccctgatgat ttctgccggg ctaccggcta
      961 gttctctttc gcagtttgcc aggcggtata ggtattttca ggaggaaaag actcggcatg
     1021 tttgggatta ttaagctgac aattcatacg attacgggaa tgtgggtcag tattgtgctg
     1081 ttcaaattga tgacgaatgg ctggagtggt ttctactttc agtgctgtgt actaagcctg
     1141 gtattcctga cggttagctg gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct
     1201 gaaccttcac gttcaacctt actctcattt acacgttacg cttttttaaa gcgggcaaag
     1261 agatgttcca ctacaacgaa aaagaccgga acaaagtaaa ttgccagcac tgttgcggca
     1321 aacatcccgc ccatcacgcc ggtacctacc gcgttttgcg cgccagaacc ggcaccatga
     1381 ctgataacca gcggcagcac gccgagaata aaggccagag aggtcatcag gattgggcgt
     1441 aaacgcatcc gcgccgcttc gatgattgcc tctatcggcg ttttcccttc tttctgcatc
     1501 atctcaacgg caaattcgac aatcaggatg gcgttcttgg cggaaagccc gatggtggtc
     1561 agcaaaccaa cctggaagta gacgtcatta cttaagccgc gcagatcggt ggccagtaat
     1621 gcgccaacga cgcctaacgg aacaaccaac atcaccgaga acggaattga ccagctctca
     1681 taaagtgcgg cgagggcgag gaacaccacg accagtgaaa tcgcatacag cgcaggagcc
     1741 tgatttgagg ataacgcttc ctgatacgac agtccggtcc atgagtagcc gacgcctgcc
     1801 ggaagtttag cgaccaggtc tgccataaat ttcatggcgt caccggtact tttcccggcc
     1861 gccgcttcac ctaaaatctc cattgacggg atgccgttgt agcgttccag tcgcggtgaa
     1921 ccataggtcc attcggtaga cgagtaggca gaaagcggtg ccatcgtgcc agaggcgttg
     1981 cgtacatacc attggttgat gttatccggc aacatacgga acggcgtgcc tgcctggaca
     2041 tacacttttt tcacccgccc ctggttgagg aagtcgttca cgtagctgct gccgaaggcg
     2101 gtggaaattg tctggttgat atcagacagc gccacaccca ttgcctcagc tttcgcggcg
     2161 ttgacgttca ctttgaacat cggcgtatct tccaggccgt tcgggcgtac cccggtgacc
     2221 tgattcggtg attgcgctgc cagtgataac agctcgtttc gtgcctgggt tagtttttcg
     2281 tgccccaggt taccgttgtc cagcagttcc atatcaaaac ctgacgcggt acccagttca
     2341 gccaccgcgg gtaagttgaa cgggaagacg acggctttat tgatactgct tagcgcaatc
     2401 attgcccgct gaatgatcgc ggtaaccgag ttttcctcac cgacacgttc agaccacggc
     2461 ttgagactga taaacgccag accgttgttt tgcccctggc cgctgaagcc aaagccgcca
     2521 acggtaaaca ccgactgaac attatctttc tcttttgtaa gataataatc cgtcacctgt
     2581 tgcagcactt tcgtggtgtt aaccatggtg gcaccggaag gtaactgcgc ggtggtcata
     2641 aataccccct gatcctcttc tggtaagaaa gaggtcggcg tgcgcaggaa cagcaccgcc
     2701 atcccggcgc aaatcagcag gtagatcacc atgtagcgac cggtacaacg caacagtgag
     2761 cgggtgctat cggtatagtg ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgaac
     2821 agggcattag gtttgtgacc gccttccggc gcggctttca gaatggtggc gcacagggca
     2881 ggggtcaggc tcattgccac aaatactgaa agcagcatgg aggagatcag cgtgatggag
     2941 aactggcggt agatctcccc ggttgcaccg ctcataaagg ccatcggcat aaacactgcg
     3001 gaaagaacaa cggcaatacc gaccagcgca cgttggatct gccccatcga tttatgcgtc
     3061 gcttccttcg gtggtagctt atcttccgca atgacacgct cgacgttctc caccaccacg
     3121 atggcgtcat ccaccagtaa ccctatcgcc agcaccatcc cgaacatagt caacgtgttg
     3181 atggtgaaac cgaccgccga caagatcgca aacgtcccga gaataaccac cggtacggca
     3241 atcgtcggga tgattgtggc acggaaattc tgcaaaaaca gatacatgac caggaagacg
     3301 aggatgatag cctcaaccag tgttttgaaa acttcctgaa tagaaatttc gataaacggc
     3361 gtggtgtcgt aaggataaac cgtcttcaga cttgccggga aataggctga taagcggttc
     3421 agttcctctt tgactgcccg cgaggtatcc agggcgtttg ctccggtagc cagtttgatg
     3481 gcgatcccgg cagcaggttt gccgttatag cgcgccacgg tggaataatc ttccgcccca
     3541 agttcgacgc gggcgacatc acgcagcagc acttgcgaac catcttgctg aactttcaac
     3601 aggattttgc caaattcttc cggcgtttgc agacgcgtct gcacaatgat cgaggcgttt
     3661 agttgctggt ctgccgcctg tggcatgcca cccagttgac caccagaaat ctggttgttt
     3721 tgcaccttaa tctgggaaat aacatcggaa ggtaccagat tgtatttatt gagtttttgc
     3781 gggtccagcc agatacgcat ggcgtactcg gaaccaaaga gctgtacgct accaacgccc
     3841 gcggtacggc ttagcgggtc tttgatatta gacgctacat agtccgcgat atcgtactgg
     3901 ttgaggctgc cgttatcaga aataaacgcc gctaccatca ggatattact gctcgattta
     3961 tcgacgctaa tcccctgctg ctgcactgct tcaggtaatg aaggcatagc gagttgcagt
     4021 ttattttgca cctgaacctg tgcgatatca ggagatgtcc cagtctcgaa ggtcagagtg
     4081 atagaggcat tacccgccgc atcactggtt gaagacatgt acatcaggcc atcaagccca
     4141 ttcatatttt gctcaatcac ctgagtgacc gagtcttcta ccgtttgcgc atcggcaccc
     4201 ggataggtag cgctgacggt aatggtcggt ggcgcaatct gcggatactg cgcaaccggt
     4261 aagttcatga tcgccagacc acctgcaagc atcataataa tggcaagtac ccaggcaaaa
     4321 accgggcgat caataaaata gttagccatg aaagtcccct taagcctgca acgttattgt
     4381 ttcgattcgg tgctggcgtt ttcctggctg gaggaaattg ctcgtgcttt gatacccgga
     4441 cgaatgcgtt gcaaaccgga aacgatcacc cgatcgccag cctgcaagcc agaggtgaca
     4501 acccactggt cgccgatggc tttgctggct tcaatttcgc gtagcttcac gacatcgtct
     4561 ttatccagaa tgagcgccgt tgctttacct tgggcgttgt gggtgacgcc ttcctgcggc
     4621 accagtaata cattctggcg gctaccttca tccactaatg ccgtgacata catgccaggc
     4681 agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc cgtggtttca
     4741 tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt accattttcc
     4801 agattgagct gtactggcgt actgccctga acctgtttga tttgcccact ggcgacctct
     4861 tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat cgggtccaga
     4921 cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc
     4981 gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg cagattgatc
     5041 gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc attcaactgg
     5101 gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa cgatgcctgg
     5161 cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag cgagcctttg
     5221 gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga atcgccctgg
     5281 ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac ctggggacgt
     5341 atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac attgaccgac
     5401 ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggtgtt ttccgccgat
     5461 ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa cagctttctt
     5521 cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta aattgcgcgc
     5581 gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag ccagtaaggt
     5641 gggatacagg catagtgatc gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct
     5701 gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat acacatacca
     5761 ggggaataaa gtcgttttag cctctccttt agtaatcacc gggggtaatg aaatcagcat
     5821 ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac taaaaataag
     5881 atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg tacataatgt
     5941 tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg atattgcttt
     6001 catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa aaacataatt
     6061 gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt ttgatacttt
     6121 ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg tgataaggtg
     6181 ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct tcttcatcaa
     6241 ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct acttttttga
     6301 tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc caaaagccct
     6361 gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta gccgttatcg
     6421 tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg ttcaatatag
     6481 taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca ctaatcaata
     6541 cttcagattt atccatcacg cactccatat attgatccta agtaagattc ctggttgtta
     6601 tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca agaataagtt
     6661 atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaaagt aatccgattt
     6721 aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc tttcttttaa
     6781 cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta tatagcgtct
     6841 ggagacatgg atatttatgc aatgtaatca acgccagttg caaaaaaatg aacccggatt
     6901 acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact ttcccacagc
     6961 acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt ttgaaatggc
     7021 taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat aatcactagc
     7081 aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc ggagagagta
     7141 ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg gataattatt
     7201 gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat atcagttcaa
     7261 ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca aggaatatcc
     7321 aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct ttcattgaac
     7381 gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa ccggcaataa
     7441 atgccgcaat ggcaaaaatg cccagctccg gtgcgcggat gaagaaatag ccgatcaata
     7501 aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga ctgcggttgc
     7561 tgaataatcc aacaataagc gcaatgcccg aacagattaa taatgcacct actactgtgc
     7621 ttaaaatatc gccagaaacg aacgggaaac tgatacacag caacccgacg ataaacagca
     7681 gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga tcaaacttca
     7741 aaattgttgc cttatctata tataacatag aaccacccta taaaattaag aagaaaatcc
     7801 cctgctatca atc
//
LOCUS       DABSYT020000160         7778 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.161, whole genome shotgun sequence.
ACCESSION   DABSYT020000160 DABSYT020000000
VERSION     DABSYT020000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.161"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            54..737
                     /locus_tag="ID725_RS22890"
                     /old_locus_tag="pgaptmp_004578"
     CDS             54..737
                     /locus_tag="ID725_RS22890"
                     /old_locus_tag="pgaptmp_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853031.1"
                     /translation="MKLPVKLLMSLISLVRVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            753..1163
                     /locus_tag="ID725_RS22895"
                     /old_locus_tag="pgaptmp_004579"
     CDS             753..1163
                     /locus_tag="ID725_RS22895"
                     /old_locus_tag="pgaptmp_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853032.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            1384..2205
                     /locus_tag="ID725_RS22900"
                     /old_locus_tag="pgaptmp_004580"
     CDS             1384..2205
                     /locus_tag="ID725_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGV7853033.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2287..2766
                     /locus_tag="ID725_RS22905"
                     /old_locus_tag="pgaptmp_004581"
     CDS             2287..2766
                     /locus_tag="ID725_RS22905"
                     /old_locus_tag="pgaptmp_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGV7853034.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSSIMRIID"
     gene            2781..3257
                     /gene="yeeS"
                     /locus_tag="ID725_RS22910"
                     /old_locus_tag="pgaptmp_004582"
     CDS             2781..3257
                     /gene="yeeS"
                     /locus_tag="ID725_RS22910"
                     /old_locus_tag="pgaptmp_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGV7853035.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3320..3541
                     /gene="yeeT"
                     /locus_tag="ID725_RS22915"
                     /old_locus_tag="pgaptmp_004583"
     CDS             3320..3541
                     /gene="yeeT"
                     /locus_tag="ID725_RS22915"
                     /old_locus_tag="pgaptmp_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGV7853036.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3615..3983
                     /gene="cbeA"
                     /locus_tag="ID725_RS22920"
                     /old_locus_tag="pgaptmp_004584"
     CDS             3615..3983
                     /gene="cbeA"
                     /locus_tag="ID725_RS22920"
                     /old_locus_tag="pgaptmp_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HGV7853037.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            4072..4445
                     /gene="cbtA"
                     /locus_tag="ID725_RS22925"
                     /old_locus_tag="pgaptmp_004585"
                     /pseudo
     CDS             4072..4445
                     /gene="cbtA"
                     /locus_tag="ID725_RS22925"
                     /old_locus_tag="pgaptmp_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4442..4636
                     /gene="yeeW"
                     /locus_tag="ID725_RS22930"
                     /old_locus_tag="pgaptmp_004586"
     CDS             4442..4636
                     /gene="yeeW"
                     /locus_tag="ID725_RS22930"
                     /old_locus_tag="pgaptmp_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGV7853038.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4682..4762
                     /locus_tag="ID725_RS22935"
                     /old_locus_tag="pgaptmp_004587"
                     /pseudo
     CDS             4682..4762
                     /locus_tag="ID725_RS22935"
                     /old_locus_tag="pgaptmp_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5051..5197)
                     /locus_tag="ID725_RS22940"
                     /old_locus_tag="pgaptmp_004588"
                     /pseudo
     CDS             complement(5051..5197)
                     /locus_tag="ID725_RS22940"
                     /old_locus_tag="pgaptmp_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5251..5433
                     /locus_tag="ID725_RS22945"
                     /old_locus_tag="pgaptmp_004589"
     CDS             5251..5433
                     /locus_tag="ID725_RS22945"
                     /old_locus_tag="pgaptmp_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGV7853039.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(5534..5863)
                     /gene="tmaR"
                     /locus_tag="ID725_RS22950"
                     /old_locus_tag="pgaptmp_004590"
     CDS             complement(5534..5863)
                     /gene="tmaR"
                     /locus_tag="ID725_RS22950"
                     /old_locus_tag="pgaptmp_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGV7853040.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6035..7093)
                     /gene="yeeA"
                     /locus_tag="ID725_RS22955"
                     /old_locus_tag="pgaptmp_004591"
     CDS             complement(6035..7093)
                     /gene="yeeA"
                     /locus_tag="ID725_RS22955"
                     /old_locus_tag="pgaptmp_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGV7853041.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(7292..7765)
                     /gene="sbmC"
                     /locus_tag="ID725_RS22960"
                     /old_locus_tag="pgaptmp_004592"
     CDS             complement(7292..7765)
                     /gene="sbmC"
                     /locus_tag="ID725_RS22960"
                     /old_locus_tag="pgaptmp_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGV7853042.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT         2020 a         1996 c         1883 g         1879 t
ORIGIN      
        1 ctgagagaaa gttttacccc tcacctgtca attcactcac tggaaattta attatgaaat
       61 tacctgtaaa gttattaatg agccttatat ctctggtccg cgttattgca cgtgccgggg
      121 aatataaaaa ttacagccgg gatgaaataa aatactggcg atatacatca tacaaggggg
      181 ggaaacttcc ggaaggtttc actgatgaga aattttccag cgccatttac aacggaagaa
      241 tatttacaat gaaacgttta cataccctta tgttatttct ggcggttctg ttcacgggct
      301 ttaactttaa cgcagaagcg gctaccgtaa aacaagctct tagttgtaac ccggaagcct
      361 gggctgaaca acctggagcg tgtccatcaa cgtacgagtt gtacgaaggt gacgctacct
      421 acaaagccgc tatcgacaaa gcattaaaac cggtcggact gagcggcatg ttcggtaaag
      481 gcggctatat ggatggccct ggtggaggta tcacgccagt aaacattaac ggtacagtct
      541 ggttccaggg cgacggttgc aaagccaata cctgcggctg ggactttatc gtaacactct
      601 ataacccaaa aacccatgaa gttgttggct accgttactt tggtttagat gacccggcct
      661 acctggtttg gttcggtgaa attggcgtgc atgaattcgc gtatctggtg aaaaactacg
      721 tagctgcggt taactaattt aaggagtcta tcatgaaaac ttttatcaaa actttactcg
      781 ttgctgtaac tattctgttc tctgtcttcg ctactgcgaa acaagtaaaa ctgccaaata
      841 acatcaaata cgttaatact acagaagcgt tttcctgtac cgaaattgac ggtatgaatt
      901 gccagacaaa gaatcagttc aactataaag ataacagcta tgttttcgtg cttgaacgtg
      961 gtggtgcctg gtgctacgac tacactgtct cggtagttaa cctgaaaacc gggaaagcac
     1021 aaatgattga atacggggac aatcaactgt gctcaggtag caacaaaccg ttcttcgaaa
     1081 tcaaaaatgg cgtaccgacg gtaggagtca tcgacacatc cggaaaacct gtcggtgtag
     1141 cccaggacaa acttaaaatc taaggagtaa accatgcgtt tctctgcaca ttattaatat
     1201 caaaggtaaa tacagcccca gccattgtta ccggaaagcc gcttaatgcg tggctcgcca
     1261 gtgaaaggcc tctcagacaa taaaccttat tcatttcacc cgtcaggccg cgtcttctcc
     1321 gggagacgcg gcttttttca tttattgcac cagtaaatct taaccaccga taaggagcaa
     1381 agtatgcgat tagccagtcg ttttggttat gctgcaaacc agatacgccg tgaccgtccg
     1441 ctgacacatg aagaactgat acgccatgta cccagtattt ttggggaaga ccggcacacc
     1501 tcccgcagtg aacggtatgc gtacattccc accattaccg tcctggaaaa tctgcagcgg
     1561 gaaggctttc agccgttctt cgcctgccag acccgtgtgc gcgacccggg ccgccgggga
     1621 tacacaaaac acatgctgcg tctgcggcgg gccggagaga taaacggaga acatgtccct
     1681 gaaattattc tgctcaactc tcatgacggt acctccagct accagatgct gccgggttac
     1741 ttcaggttcg tctgccagaa cgggtgcgtc tgtggccagt ctctggggga agtgcgtgtt
     1801 ccacaccggg gaaatgtagt ggagaaagtt atcgaagggg cttacgaggt ggtgggcgtg
     1861 tttgaccgga tagaggagaa gcgtgatgcc atgcagtcgc tggtcctgcc gcaaccggca
     1921 cgccaggcgc tggcacaggc ggcactgact taccgttatg gtgacgaaca tcagcccgtc
     1981 accaccgccg acattctgac gccgcgacgc cgggaggatt acggtaagga cctgtggagc
     2041 gcatatcaga ccatccagga gaatatgctg aaaggcggga tttccggtcg cagtgcaaaa
     2101 ggaaaacgta tccatacccg ggccattcac agcattgata ccgacattaa gctcaatcgc
     2161 gcattgtggg tgatggcaga aacgatgctg gagagcctgc gctgatgccg tttccctgag
     2221 tgagggaatc attcagcttt cgttcccgaa aaacacacat ttatttgtta tttaaggagt
     2281 tatctcatga aaaccgtttc tcagaatacc cccacaattt attcagctac aacaccagag
     2341 aataatccgc ctcagttggt tgccagcctc gtccctgatg aacagcgcat cagcttctgg
     2401 ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg agccccgtgt cttcggctgg
     2461 atggaccgtc tgtgcgaaga ctactgcggt ggtatctgga atctgtacac cctgaacaac
     2521 ggcggggcat ttatggcccc cgaaccggat gatgatgatg acgaaacatg ggtactgttc
     2581 aatgccatga acggtaaccg cgctgaaatg agcccggaag ccgccggtat tgccgcctgt
     2641 ctgatgacgt acagccatca tgcctgtcgt acggagaatt atgccatgac ggtccattat
     2701 taccggttgc gggattacgc cctgcagcat ccggaaggca gctccattat gcgcatcatt
     2761 gactgaaagg ggccggaata atgcaacaga tttcctttct gcccggagaa atgacgcccg
     2821 gcgagcgcag cctcattcaa cgggccctga aaaccctgga ccgccatctt catgaacccg
     2881 gtgtggcctt cacctccacc cgtgcagcac gggaatggct gattctgaac atggcgggac
     2941 tggagcgtga agagttccgg gtgctgtatc tgaacaacca gaatcagctg attgccggtg
     3001 aaaccctctt caccggcacc atcaaccgca cggaagtcca tccccgggaa gtgattaaac
     3061 gcgccctgta ccacaatgcc gctgccgtgg tactggcaca caatcacccg tccggtgaag
     3121 tcacacccag taaggcagac cggcttatca ccgaacgtct ggtacaggca ctgggcctgg
     3181 tggatatccg ggtgccggac catctgatag tcggtggcaa ccaggttttc tcctttgccg
     3241 aacatggtct gctttaaccc gtcacaacca catcacacct gttttcactt ttatcttctg
     3301 tcttcagagg tatcccatta tgaaaattat cacccgtggt gaagccatgc gtattcacca
     3361 acaacatccg acatcccgtc tttttccgtt ctgtaccggt aaataccgct ggcacggcag
     3421 tgctgaagcg tataccggtc gtgaagtgca ggatattccc ggtgtgctgg ccgtgtttgc
     3481 tgaacgccgt aaggacagtt ttggtccgta tgtccggctg atgagcgtca ccctgaactg
     3541 agtgggaatt ctgatgagca gaattatcgc cactaccgga ccattcttag ccgattttct
     3601 gtaaggattt tatcgtgtca gacacactcc ccgggacaac acttcccgac gacaatcacg
     3661 accgcccctg gtgggggctg ccctgcaccg tgacgccctg tttcggggca cgtctggtgc
     3721 aggagggtaa ccggttgcat taccttgccg accgcgccgg tatcagaggc ctgttcagcg
     3781 atgcagatgc gtaccacccg gaccaggcct ttccgctgct gatgaaacaa ctggaactca
     3841 tgctcaccag cggtgaactg aatccccgcc atcagcatac cgtcacgctg tatgcaaaag
     3901 ggctgacctg caaagccgat accctcagca gttgtggtta cgtttatctg gctgtttatc
     3961 cgacgcccga aatgaaaaat taactctcca gaatagcctt ctgctacggc ctggtgtttt
     4021 caccacgcca cttttccatt tttatatctg catatcagga aaatcttcag tatgaaaaca
     4081 ttacctgtat tacccgggca ggcggccagt tctcgcccgt ctcctgttga aatctggcag
     4141 atactgctgt cccgactgct ggaccagcac tatggcctca cactgaatga cacacctttt
     4201 gccgatgaac gtgtgattga gcagcatatt gaggcaggca tttcactgtg tgatgcggtg
     4261 aactttctcg tggaaaaata cgcgctggtg ctaccgacca gccgggattc agcgcctgta
     4321 cccgctctca gttaataaac agcatcgata tcctccgggc tcgcagggcg accggcctga
     4381 tgacccgcga caattacaga acggtaaata acattaccct gggtaagtat ccggaggcga
     4441 aatgatgaca ctggaagccg acagcgttaa cgtacaggcg ctggatatgg ggcacattgt
     4501 cgttgacatt gatggtgtta atatcactga actgattaat aaggccgctg aaaacggtta
     4561 ttcactccgc gtggtggatg accgtgactc taccgaaaca ccggcaactt atgccagccc
     4621 tcaccagttg ctgtaagaca atgcagtgat gcagggtata gtgccattaa ccacagaaac
     4681 agcactgttg tcctgtatta agcaggcaag agaagatgcc cttaccctgc gccatctgca
     4741 gcttctgcac cagaaccggt gaatctcact caacaggctt catttcccct gacgccagcc
     4801 tgaatacagc tggcgttttc atttataaac agaaaggaaa accgataata atggaatgga
     4861 cgccccgacc atgaagcaac ggtaggatat tactgataaa ccttccagtg gaggtgcgtt
     4921 atggccgttt taacgattag cattgacctg acaaaaaatg aattccaaat ccacggtctg
     4981 ggtaggaaca gaaaaatcta agctcagaaa acggattaaa gtaagcgtca acggagcacc
     5041 gtattgacgc ttatttattg gtgagaacta cgttccatgg caggagttcg tcaacacggt
     5101 tggagggcca ttccggcagt atgctcagaa tatggcgcag atacgcttcc ggatcgatac
     5161 cgttcagacg gcaggtgcct gcttttccgg caacaagcag gaatctcagc gctgaagcag
     5221 gaattagagg taaaaacgcc ctaccgggcc atgaatcacc cggtcattgg agtagtaacc
     5281 aaagcagatt tagccagcat ggaacaaata tctttggtga aatgttggtt acgggaagct
     5341 ggagcgcaca acgtgttagt aaccagtgcg gttaataaca atggagttac agaacttttt
     5401 gccttgctgc atacagaaga aggctgccgc taatttcgat aggttctcag atgagaatga
     5461 tgacggtgat acacagataa gaatcaatga gcaatgaggg ttgccgggca accctcattg
     5521 aataaaacag gaattacttc gcttcgccgt ttttcatttc gcccatagct ttcagctttt
     5581 tggagatatc gcggcgttct ttggagagct cggcattttt gatgatgtaa tcgtcaacgc
     5641 gatcttcata gtcacctttc atgctggcga tgatgccctg gattgcttca acgctcatcc
     5701 ctggcttgat gtaatcgctc aggttgtcca gcaacaggac gcgcttctgg ttgtcacgga
     5761 tctttttctc aacgtcctga atttcacgtt gcagtttgtt cttacgacgg aacagacgaa
     5821 caaattccag tacgtcctgg aatgaaggct tggtagtttc catttttata cccctgataa
     5881 tgtgagagtc ggattcgttt aagcaaccgc tattcgttag ggccaacatt actcatagct
     5941 gccgcagatg acaatgcttt tatcccttat cactatcata ccctttatcc ttgctgaatc
     6001 gaagcagcag caagatgatt ctgaagttca ggaattattt gcgcaaggcc cggcaaatca
     6061 gactcgatag caactcaagc tgatgtgccg tttccatgtt cagccacaca taaccataga
     6121 ttggtgtttc cacaaccttc aggtcatggt ggttattcag caactgacgc agctcttcca
     6181 cagcatcgtt caatttttcc gtattggcaa aaaccggctg cggattacct tcgtacagcg
     6241 catgaacaag gctcagcagt atttgctgca tcatgtgctg ggtatcacga agtttttgcg
     6301 cgttcaataa cacgaaatgg ctggggcgcg tggcccagta tgcattgatt tgcaactcca
     6361 gcatacaaac cagattgcgg ttaatggtct ggataccttc atatatcgat tttggaatac
     6421 gagtttcttt gctggcgggc gcaatcagcc cacgcatttt cacggcatcg gtcaggagtt
     6481 tttgtagatg gctttccaga cgtgggcgtt cgagtaagtt cggtgagaat gcagattgat
     6541 agacccgatt atactcggtc agacttttcg ccagttgaat gcgccagtgg atgaacgccc
     6601 gttgtggcca gataccggta aacaacattg ccagtaaaga gccgaggatc acatcgccgc
     6661 ttcgccataa cgccgtatca atttcacctg tcggggaacc cacaacaatt gccagcgtca
     6721 ccccaatcaa taatccttga tacggtttct tgcccagcgc cagccaaccg caaagaaaca
     6781 tcgccgccgc gcaccagact aacatcagcg gtaacgaaat taactccagt tgcagagcga
     6841 taagacctaa aatcgacccc aacaccgtac cgccaatacg ttcgaaggca cgagggacaa
     6901 cgttccccca gaacgagatt ggccccataa tcaccaccat ggtgaccagc ggccaggtgc
     6961 cttccgggat agtaaacagg cggataatga gaaaagtgag caggaatgcc agcgcgaccc
     7021 gagtaccatg cacaatgcgg taatggcggt atacccggat ttcaaacggg cttaatgact
     7081 tattggcacg cacgcagaaa ctccgttaat gaagattaaa agcgctcata acacatctgt
     7141 cggtcagaag gcgcgcctct gaattatccg acggacaaac cgggaacagt gtaatttccc
     7201 ggcaggagcg aaaaaattga aaggcgcatc ttactctttt cgctttcaaa aaaagagtgg
     7261 tcttcgcgtc aacacaccgc cctgagatga attagtgatg ttttggctgc accgcaacat
     7321 acatttcgat atcccagtac ccatcttccg cgccattgtt caagtaaacc tcgaagcagg
     7381 gctttggtaa catttcataa gcactgtcct gcaagaggct attaaagaac tgataccagg
     7441 gtttagcaaa atcatcaccg actacacgag ccaccgccac cgcatattga ccacctgaaa
     7501 tttctgtcag aatgacgccc tcactgtttt cggggagcgt aaagttattc ggtaccgtca
     7561 cgacggtgtc gcagcgtaat ttttcggcgg gtgtttcatc cggattgtcg taatagacag
     7621 caacccactc cttcggcaca atatttttgc tatctaccca catcatcaac tgctcaaaac
     7681 ctttctttac cgtctgttcc cacgggccaa cgagatggaa acctgcaacg gtacgtttat
     7741 cttcctgctt aatctcgtag ttcatgacgc ctccatta
//
LOCUS       DABSYT020000161         7772 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.162, whole genome shotgun sequence.
ACCESSION   DABSYT020000161 DABSYT020000000
VERSION     DABSYT020000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.162"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..91)
                     /locus_tag="ID725_RS22965"
                     /old_locus_tag="pgaptmp_004593"
     CDS             complement(<1..91)
                     /locus_tag="ID725_RS22965"
                     /old_locus_tag="pgaptmp_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853043.1"
                     /translation="MDEVKKIEHCKILNTDTPDNSASDLDSFLK"
     gene            complement(69..527)
                     /locus_tag="ID725_RS22970"
                     /old_locus_tag="pgaptmp_004594"
     CDS             complement(69..527)
                     /locus_tag="ID725_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HGV7853044.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            complement(517..1809)
                     /gene="sctN"
                     /locus_tag="ID725_RS22975"
                     /old_locus_tag="pgaptmp_004595"
     CDS             complement(517..1809)
                     /gene="sctN"
                     /locus_tag="ID725_RS22975"
                     /old_locus_tag="pgaptmp_004595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HGV7853045.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            complement(1841..3901)
                     /locus_tag="ID725_RS22980"
                     /old_locus_tag="pgaptmp_004596"
     CDS             complement(1841..3901)
                     /locus_tag="ID725_RS22980"
                     /old_locus_tag="pgaptmp_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HGV7853046.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIVVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQETKYILD
                     QLEMKYSDLLKEILRYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKDIIT
                     LVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEM
                     ILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNI
                     LKTI"
     gene            complement(3894..5039)
                     /locus_tag="ID725_RS22985"
                     /old_locus_tag="pgaptmp_004597"
     CDS             complement(3894..5039)
                     /locus_tag="ID725_RS22985"
                     /old_locus_tag="pgaptmp_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN/LcrE/InvE/MxiC type III secretion system
                     gatekeeper"
                     /protein_id="HGV7853047.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLNNALVTLPADANYKDKTLLLKAIYSGCTNLPFSLF
                     INNEQLLEIRECCKQAIKVTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            complement(5044..6747)
                     /gene="sctC"
                     /locus_tag="ID725_RS22990"
                     /old_locus_tag="pgaptmp_004598"
     CDS             complement(5044..6747)
                     /gene="sctC"
                     /locus_tag="ID725_RS22990"
                     /old_locus_tag="pgaptmp_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HGV7853048.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(6744..7493)
                     /locus_tag="ID725_RS22995"
                     /old_locus_tag="pgaptmp_004599"
     CDS             complement(6744..7493)
                     /locus_tag="ID725_RS22995"
                     /old_locus_tag="pgaptmp_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7853049.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT         2269 a         1707 c         1293 g         2503 t
ORIGIN      
        1 tctttaagaa actgtctaaa tccgaagcag aattatcggg tgtgtctgtg tttaaaattt
       61 tacaatgttc aattttcttg acttcgtcca ttatattttt cctcctgttc aagctcgtct
      121 tgataaagca tctgatttaa aagttgtttt ttgatgcgct tttttagtcg gtcgtatttt
      181 tctcctttta gccaccagtg ttttctttgt gcttgctgtt gaactatttc cttgttaagt
      241 atttttcgcc gctcggctat tttttgcttt tcaagattta tctcagcgat ctgctgctga
      301 atgactgtaa ctttacgtaa cgagtaaaat atatcctggc gaaatacaac ttcgccgagt
      361 aactccttca tatctaacag tgactgcagg tttttaagtt gcatatcata caggcgttcc
      421 ttttcggcta acttttcttt ttctgcattt aacttttgta atgatgcttc acatgcagcc
      481 aggctctgcg ctgttcttct gataagtcga ttaactttac ttaacaattc gttcaagctc
      541 ttttagcgtt tcactcattg gcattttttc attaactgac tgtttcaacc aatttgtgag
      601 tttaggccgg gcattcatag caaaatcatt ttcagcattt tgccctgcgc gatattcccc
      661 taagtcaata aacacctgta gatcttccaa agttgtgagg ttcttcctta cccgtgcagc
      721 attatctctg tgtttttcat cagtgacctg accaaatact cgactcacac tttttagaac
      781 atctattgcc ggatagtgac cttgacccgc gagtttgcga cttagataga tatggccatc
      841 aaggattgag cgaatttcgt cacccagtgg gtctgattca tcttcccctt ccagtaacac
      901 ggtataaaac tcggtaatac ttccttttag tgttggtccc ggcctctcca gtaagcgagg
      961 caaactgtca aaaacagaag caggataacc acgtctggca ggtggttcac ccgctgccag
     1021 tttcatatca cgtagtgctc gtgcataccg cgtcattgag tcgataaaaa gtacaactct
     1081 ctttcctcga tctcgaaaat actctgccac ggttgtcgcc atcagggcgg cattacaccg
     1141 gtctactgac gaaaaatcag aggtggcata gaccaatacg catttcgctg catttactga
     1201 ttttttgagc gattccgcgc attccgtaac ttccctacca cgttcgccaa tcaagccaat
     1261 aacaaacaca tcagcttcag tattgtttac gagcatatgc attaaaaccg ttttgccaca
     1321 gccagccgag gcgaaaatcc caacacgttg accaatgcca catgtcagta cgccatcaat
     1381 gactcttatc cgtgtctcaa gtggtgtatt aataactcga cgttcctgat acgacggtgg
     1441 cagttcatca atcaggcgta aatttcccct atcgcctgga gtgtccggga caaacctttc
     1501 cataatttga ccagaggcat tcaaaaccga accaagaaag gcatcactga tctgtatggt
     1561 taactgctct cccgttggtt tcaaaactgc agtacgtgta agccccacag agctgcctat
     1621 aaggtttaat aatgtcttcc cattgcgaaa gccaacgacc tgagccctgg ctatacgttc
     1681 atttgcttgc ctgctcgcat aaatttcgca tacttcaccc acagaaacgt cgtcaagttc
     1741 tgcttcaatc agagagccgt ttatgctatg taagtagctg tatttattta ataattttaa
     1801 ttttttcata ccagcatgct ccatatcgca agacattatc ctaaatagtt ttcagaatat
     1861 taacgggaac attttccgtt agttccccgt acgaaagtac ttcgaggtct cgataactag
     1921 attcaataag ttttttgata aaacgacgaa tatccacaga tcccagtaaa ataatgtctt
     1981 taataggaat attgattcca gaaagtgcca gtttaaaatt atccagaatc atctcagctt
     2041 cggatgcatc gagattaaga aaagtaccgc cagcggtagg cctgacacca tctctgattc
     2101 tgtcctctat ttcaggcgaa atcacaattg ctttaatttc tccagaatat gaaaacttat
     2161 ggcaaatgta gcgacctaat gcacctcgta catgctcgac gagtgtgatt atatctttct
     2221 ctcgtgggct ccataatgct aaggcctcca taactaaccg catattgcgc actgaaattc
     2281 gctcctggat cagacgctga attacctctg aaatgcgctg cacggttata tatcttaata
     2341 tttcttttaa tagatcagaa tacttcatct ctaattgatc aagaatatat tttgtttctt
     2401 gtataccaaa gtactcatta atattatgag ccagacagac agataaatga ttatacattt
     2461 catccatggc cgtgcgagta taacagccaa tttttgttaa tttttgagca tcacttttcg
     2521 taacccaata atattgttca ccattactat ttacaatgac aggtttcttg atcaaagtcg
     2581 aaaccacttc atctgaatat tcaaccaaca agacaagatc atgataaatt ttaaattgtg
     2641 aagctctgac ttcatttagc attaatatga catcttcatc attaagacct tccccctcac
     2701 gaatgacaat accgggaatg cggataccat actcaataaa aaactgacta cgaatcctat
     2761 cagcaagatt gatttcagtg tattttttag cctgtacact attcataatc aagaccaagg
     2821 gtaccgtttc ggtaataacc tgatcaagtt tattaatgat acctaatgag atatcatcat
     2881 cttgattata gggttccaac ccaacggtaa tatctccctc aacaaccgtt tcttcagtcc
     2941 ctttcttttt gaatttaaag taaaagtaaa ttcctaagat tactgccaaa cataaaaaca
     3001 ctagaagggg aaaacctgga agcaacccaa tacttagcgc gaggacacat gtcacaacaa
     3061 tgacaaaaga attacttaat aactgggaca ttatattttg ccccatatta tcactatcgc
     3121 catttacgcg cgtaacgata aatcctgcgc taatagcaat taataaagct ggaatttgag
     3181 aaacaagacc atcaccgaca gtaagaattg tgtagacagt caacgctgtt gagaaatcca
     3241 ttccattttg gcctactcct actgatagcc caccgataat attaacaaag ataataataa
     3301 tgtttgcaat agcatcacct ttgataaatt tcatcgcacc atcaaaggaa ccatacaact
     3361 ggctctctcg ttctaataca ctacgacgtt cttttgctaa atctgcatca atgatcccgg
     3421 cgcgtagatc ggcgtcaatg ctcatttgtt tacctggcat accgtcaagc gaaaagcggg
     3481 cagcaacttc agccacacgt tccgaacctt tagtaatcac gataaactga acaatagtaa
     3541 caatggagaa aataacaaaa ccaactacca ggctatctcc aataacgaac tcaccaaaac
     3601 tcgttataat ttctccagca tccgcctcaa gaagtataag tcgactggta ctgatcgcca
     3661 gcgccaaacg aaataacgtt gtgataagga ggattgatgg aaatgaagaa aagcttaaga
     3721 tcctatcaac atagaatgat cctaaaaata caagtattgc caatgttaag ttaagcccaa
     3781 tcaaaaagtc gattaaatag gtgggcaatg gaattatgag catggcgata atcatcacca
     3841 ttaatccaag aattattaat tctggatgac tccttaaccc taccaaaact ttgttaaaca
     3901 ttgaacatca ccttcttcca tggtttttta ttttgttttt tgttacagtt ttgtgtatca
     3961 aagagctctg ctgcaaatgt tacttttatt gcctgtttac aacactccct aatctctaat
     4021 aattgttcat tattaataaa aagtgaaaat ggaagatttg tacatccact gtaaatcgcc
     4081 ttcaggagta acgttttgtc tttataatta gcatctgcgg gaagggtcac cagtgcattg
     4141 ttcaggagtt cctctgcagt ctcaggaaat tttattaacg atatcagaaa aaatatccag
     4201 taatcttctg cgctgataaa ttttttaatt attgggttat tcattaaagt tttcagaaat
     4261 aaagcctctg ccgactgaag agacaatagt tgtgacaact tattgagaaa cataccaaat
     4321 tccaatgatg aacaactcgc atcaagtgca tttatatctg agaaaagcgt ctctttgata
     4381 aattttgtca ccaacattct attttgatag ccataactac caatccattc gacataagta
     4441 tctacagcat cgtcttctgc cattaaaaat tgccgataac tcgcccttag taattttgga
     4501 tttaatgaca gcgcctggcc aaataacttt gcttttaatg ccgagttaat tccgctatta
     4561 atgaattttt tagtagtctc ttgattaact tttctaagta atgcttcagc gtttagccgg
     4621 acattttctg atatttgttt tttacgtaaa attgcacgta acacaactac cagatcgctt
     4681 tcatctggga ataatttttt agcaaagcta tacacttcat agtcatgggt agttgcagcc
     4741 ttaagcgcct ccataacttt gtcggttttt tctattggct ggtcttccag gacatattct
     4801 tctccgtcca gaaaattggc cagttgttct tcgtaaattt ttttcttcat aaactgcgtt
     4861 aacgctgctg acatctcgtc agtagcttgt aagaaccgag cttctttacc tgcgggatca
     4921 tccctcattt taggcatttc agcttcaatg tcttcattat cttctgtgat aatgtcctct
     4981 aagcgagaaa cttccctggc tctttctaat acaccgacat gctctacatg aatcgccatt
     5041 tcactatcct ccgacgactt cacgattcag gtaagttttt atccatttct gcagtaattc
     5101 atcgttgatt tctttatttt ttgccatttg ctgtgtaatc gctctggcat caaccgcagc
     5161 ctcattcgca ttattcatca tgcgttcatc gatctctctt ggctcaataa gaaagacacg
     5221 aacgatgtta ttcgcatttg ttccttcata accaaataat ttaccaataa atggaatgct
     5281 gcctaatatt gggatcttgc gactttcata ggtattcgta tcgcgtgtat atcccccaat
     5341 caaaagactt ttcccttggg gcacgcgtgc aatagtactt ataagtgttc taccaacctg
     5401 aggcagatca tcaacattgg tcttgtcgga attgatttca tttccatctt caatattcaa
     5461 gagtaattca atttgatttc gcgcagcaaa tcgtggtagc acgctaatca tcgtaccata
     5521 ggttacttca tccaattccg cagtacgctc gccgactaat ttagtataga aagtgcggtt
     5581 attatcaaat atagcaggaa tattctcttg tgttaaaacg acgggacgag caacaacggc
     5641 ggctcttctt ttttgtgcaa gtgcctgtat tgttgcaata aactgtgtcc catcaagggt
     5701 actaattgaa cctgaattat taaatgatgc acttaagctg gatccaattt ttatcgtgcc
     5761 cgaccagtca gcacctagct gttctagatc agtcttatca atatctatta tccataaaga
     5821 taattcaatg tgtcgctttg gaatatcaag cgtagctact aacttctcga taaaatcaac
     5881 ctgagatact gttcctttaa caagtaaact gttggtatca ggatatgcaa taatttgtat
     5941 atcgtcagtc tctggcgctc ctgctgtcga tattattttc tctacttgta ggtcatcatc
     6001 ttcttcagcc ccaggaaaag gaggcatacg tgatacgttt tttagttgtt gacgacttga
     6061 aatttcagaa agtacgttca cccctgtgct ttgcttaatg ttattattta acaacgtcga
     6121 tagtactttt gccatgccag gaataactat cttttcgcca cgtaactcat aagtccggtc
     6181 attaacgaat gtgttgacca gatgaattat tcctacttta tttcgaccga tctcaatgcc
     6241 atcgctgttt tgctccatta atttcgcagc attaacgacc aggtcaacat aaaccggtgg
     6301 ccccgataca tagaaagtac cattgccgtc ccctttaatt tcataacgag agttatacag
     6361 acccgatttt tttaagaaat tattaaactc attcgtcgat acctttctca gattaatcaa
     6421 tgcattacgc atctctgatg agtcatagat ataaatagct ttgccatcat cataccaaat
     6481 aagccccatc tgtgccgaca ggcgttcaat caaggcatag ggttctgtta aatcaaaatt
     6541 tccgctgatc tgtttcttca tggcaagctt actaactatt gtaggcttac ccgcatatga
     6601 tgaaaccgtt tcaaaaaaac tacgtagatt ttctttattt gctacgtatc cattcgctgc
     6661 tgcggcatat gcaccaggag tcaataatcc attaaaaatg cataagactg aaattaatat
     6721 aatagtaatg cgtaatttta ttttcataac tttttacata gttctctcgg tgtttttcct
     6781 aaccggcttt ttacttcggc tgaaaagtgt gatgaggacg agtaaccata tttatgcgca
     6841 atagttgtca tatcactgtt tccttcaatg atttccaaca cggctctggc aactctccag
     6901 ccacataatt ccgttttaac tttaccaccc aatgcataac tacataaacg gcgaaaatgt
     6961 gaatacgata cgccgtaaag ctcaccaaga tcatagagtg aagtctgtat gagcgattgc
     7021 gataataaat aacgaaccag tttatagcat tctgtttttc ttagatgctg aaacaggatt
     7081 tcattaatat tatcgctgct cagataagtg gctaaaaacc aggcttcact tgcaacttta
     7141 ctgaatagca caacttctga tgcaaagggc aaggcatcag caaacaattc atggtgttcc
     7201 ctttccacac tccccgttga ttcatctata aaagctaata atttactcag gatatcaatg
     7261 cgaagtggat gataaattac tttccctgca gttacctcaa tttggcttcg gtcaatgagc
     7321 agtaatatat cttccactac cattaatttt tgatggttat taccaatcgt aataacaagg
     7381 gttacatcct tgttttcatt tccccgtaag aaccacattt cagggtccag tagggttaac
     7441 ttctgtcctt gaagcaaaac attcattgga agtaacagcc cttcttcaat catgtttcat
     7501 ctccttattc catattctca ttctccataa acttgctata aaaacatgat taaaaagtga
     7561 tgattactat ttataaaata atgattttgt tatgagcgaa tagctatcag ccacttacac
     7621 tagtacgtca tcctgtccat atacagattt gtaactgttg atatgtttta cttaaacaca
     7681 cctcgtatta cttataacac ttgtttggta actattttca atacaaatca tatttgttag
     7741 ttcttcatta acacactgcg agtgttttgt ta
//
LOCUS       DABSYT020000162         7450 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.163, whole genome shotgun sequence.
ACCESSION   DABSYT020000162 DABSYT020000000
VERSION     DABSYT020000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.163"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..245
                     /locus_tag="ID725_RS23000"
                     /old_locus_tag="pgaptmp_004600"
     CDS             <1..245
                     /locus_tag="ID725_RS23000"
                     /old_locus_tag="pgaptmp_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853050.1"
                     /translation="SYDDCFLSCNAEKLKIKSQLKGLIESIGSNVREHELIMLRKNDV
                     NEIYQSEKSDFKTLEDKNIKQYIHLLNHFMNIKTIF"
     gene            245..811
                     /locus_tag="ID725_RS23005"
                     /old_locus_tag="pgaptmp_004601"
     CDS             245..811
                     /locus_tag="ID725_RS23005"
                     /old_locus_tag="pgaptmp_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853051.1"
                     /translation="MDHTKHSILSSLQDKEDDVDELKYSAEDFDSLTVADLYDIEIAM
                     QDFLNDINFENSKDNKVRFDEDTYDFNINGKRRGMFGKGTRAVMHAIFTICFAEFLSR
                     KGNPFIGFVVLDSPLVTHFDKDRGGSLSDVNSVSLSDSFYHALIKRDYNFQIVILENK
                     GPTFQIKINDANKIHNLNKNGSSGFYPV"
     gene            complement(1221..1493)
                     /locus_tag="ID725_RS23010"
                     /old_locus_tag="pgaptmp_004602"
     CDS             complement(1221..1493)
                     /locus_tag="ID725_RS23010"
                     /old_locus_tag="pgaptmp_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGV7853052.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(1499..2050)
                     /locus_tag="ID725_RS23015"
                     /old_locus_tag="pgaptmp_004603"
     CDS             complement(1499..2050)
                     /locus_tag="ID725_RS23015"
                     /old_locus_tag="pgaptmp_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HGV7853053.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(2047..2799)
                     /locus_tag="ID725_RS23020"
                     /old_locus_tag="pgaptmp_004604"
     CDS             complement(2047..2799)
                     /locus_tag="ID725_RS23020"
                     /old_locus_tag="pgaptmp_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HGV7853054.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
     gene            3733..3993
                     /locus_tag="ID725_RS23025"
                     /old_locus_tag="pgaptmp_004605"
     CDS             3733..3993
                     /locus_tag="ID725_RS23025"
                     /old_locus_tag="pgaptmp_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7853055.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
     gene            3990..4547
                     /locus_tag="ID725_RS23030"
                     /old_locus_tag="pgaptmp_004606"
     CDS             3990..4547
                     /locus_tag="ID725_RS23030"
                     /old_locus_tag="pgaptmp_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGV7853056.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            4544..4765
                     /locus_tag="ID725_RS23035"
                     /old_locus_tag="pgaptmp_004607"
     CDS             4544..4765
                     /locus_tag="ID725_RS23035"
                     /old_locus_tag="pgaptmp_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853057.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            4765..5088
                     /locus_tag="ID725_RS23040"
                     /old_locus_tag="pgaptmp_004608"
     CDS             4765..5088
                     /locus_tag="ID725_RS23040"
                     /old_locus_tag="pgaptmp_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HGV7853058.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            5102..7435
                     /locus_tag="ID725_RS23045"
                     /old_locus_tag="pgaptmp_004609"
     CDS             5102..7435
                     /locus_tag="ID725_RS23045"
                     /old_locus_tag="pgaptmp_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGV7853059.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
BASE COUNT         1848 a         1725 c         1842 g         2035 t
ORIGIN      
        1 tatcatatga tgattgtttt ttgtcgtgta atgcagaaaa attgaaaatc aaaagccagc
       61 tgaaaggatt aattgaatca atcggatcta atgttcgcga acatgaatta attatgttaa
      121 gaaaaaatga tgttaatgaa atttatcaat cagaaaaaag tgattttaag acgttagaag
      181 ataaaaatat aaaacaatac attcatctct tgaatcactt tatgaatata aagacaatct
      241 tttaatggat cacacaaaac atagcattct atcttcatta caagataaag aagatgatgt
      301 tgacgaactt aaatactccg ccgaggactt tgactcctta acagtagctg atttgtatga
      361 tatagaaata gcgatgcaag actttttgaa tgatataaat tttgaaaatt ctaaagataa
      421 caaagtgcga tttgacgaag atacatatga ctttaatatc aatgggaaac gccgaggaat
      481 gtttggtaaa ggtacccgtg cagtaatgca tgctatattt accatttgct ttgcggaatt
      541 tttatcaaga aaagggaatc cattcattgg atttgttgtg cttgatagtc cattagtgac
      601 gcactttgat aaagatagag ggggttctct atcggatgtt aatagcgtca gtctcagtga
      661 ttcattttat cacgcattaa tcaaaaggga ctataatttc cagattgtaa ttcttgagaa
      721 taaaggtccg acgttccaaa taaaaattaa tgatgcaaat aaaatccata acttaaataa
      781 gaatggaagc tctggttttt atcctgttta atacattaaa aatataactg ctaattgggg
      841 tgctcgaaat tatatttcgt ttcgagcgct cagttcattt ctaaataatt tgcaaaatta
      901 ttttgggtac tttcgcctct acacttcctt cgggtggttc cacaataatg gatctcaccg
      961 gcgtttgata ttttataatc taacccctta gcgcgcgcag ccccccacgc ctgctcgctt
     1021 cgcttaacag actggttttc atgcattccg caaatcgtct cagaagccac cacacaaggg
     1081 ctttcgcgtc aaaaatggtg catgagaatc atgcgttttc atgcgctata gacatgcact
     1141 catgcgctct caggccagcc agggaaaagg cgtaaaaaat cccagtactg gaccgggatt
     1201 acgtgggctt tttttgctaa tcagacagga atttgctgac ggcttgacag ctttgacgcg
     1261 gagccatagc gattgagagt ttgctgttgt ttttccggca tttgtgcttt ctctgattcc
     1321 tgttcattgc gccggggctt catgattatc gtatccacac tttccagtgc ggtaaatgtg
     1381 taggaacatt caagattctg gcactgatac catgagcgtt taaccgatgg tgcttcatag
     1441 gcggaggttc tggcgtgtgc cgtcgtaccg cattcgggac atttaagtgc cattaatatt
     1501 actcctgcat gccgttaatg taatccagac gttgctgaaa gcgcagttgt tgtgcagggg
     1561 tgtaagagct ctggcaatca cgaaccatca tggggtcagg catcacaccg gattcatcca
     1621 gaatcatacg ataatccccg gcaatttcag gcggtgttat tgccagctgg cgaaccagag
     1681 cattgcgtaa tatacgggac gcaacgtcaa cccctccgcg ccccttcagg aacggagcca
     1741 gtgcagatgt cagggctgct ccattcgcct gcataaaatt gctgagagcg tcttctgtgc
     1801 aggcctccat cagttgatgc tgcgagtaaa tgcattcgcg cgcagcacag ttaatttgcc
     1861 atttcagcac atccagatgt tcccgctgta attcgagctg tcgagcataa tctgctggtt
     1921 tttccatggc agtgaggagt gattctatct gttgttcaac ggcttttcgg ctctcactat
     1981 gtccagccca cagaatacgg gcatttctaa aactgataag cgcttggtct ggagtgactc
     2041 gggccatcat actgctttct ccttctcagc acgttcctga cgaaccttca ttttatgcat
     2101 agctgctgga gagagtttat tactgaaatc cagacgggcc gcataatccg gggcaactcc
     2161 cgccagttta aacacggggt cctgttcggg catcgcatcg ttagtcagag ccggtctggt
     2221 gatatgttta tgaataaaat ctttcagcag tgtgtccggg tcattaactg aatgcaccac
     2281 accgacaact gcgctggctt ccctgcccat tgttgatttg agcaggctta gtgtctggat
     2341 tagtgctttt ccatgcgact gcataaaatc ttcccagatt tgttttgcgc gaatacccac
     2401 aagggtgtcg tgcgcgtgga tatactttcc ggctaattcc gcagcctcct gcggcaaaag
     2461 tgcattttcg ctttcctgag ccgccagcag ctcatcaaaa tcctcaagcg tttctcgtcc
     2521 cagcgcaatt tccgtgcgca gttttttcat ttcctttgag actacgccct gactttcacg
     2581 aaatagcgtg cgccactcct cattcagggc gttcgtcgtg gcttccattt cagcgcggcg
     2641 ctggcggatt gtcgcaatat tatccgctgc cgctttttgc tgacgacgtg cttcaagcca
     2701 tgcagatttg gtggcattca ctttctcaag cgcctgttgc gtcgaaacag ggatagaaat
     2761 ttcaacgatc gggttattgt tctgttctgg gattgtcatg tcagcaccta gtggtttgtc
     2821 gtggtgtcaa ttgtgctgtg atgcatacaa agctgccatt gaacgccatt gtgcgagcga
     2881 caagacaaag actgaaccgc cccggacctc ttttggctag ccagaaaagg cctcgtttaa
     2941 ctaaggctgt ttcagactta ccttaactat tcactactat tcacttaggt aaaaaaaata
     3001 ggtaatacag aaggttataa ggtgaagggt tggcatggca gtattcatcg actgttcata
     3061 actgttcaca tgggaataaa attatttatg gtattttttc ataatgggtg gtttttttat
     3121 tcctcgtagc tatttgtttt ttgctcctca ccactattca cccttattca ggagtattca
     3181 acaggcacat aaatagactg catgtttttg ccggggaata acattttcgc ctctggccat
     3241 ggtgttgcat ttcatactga tttttataaa actagatctg tccggttctt tatggattca
     3301 ttcggatata ttttacgatt taaggtgatg ttatttaata tcaaggtttt tatgcaggcc
     3361 caatgtttat aagttggatg cgattcaaaa atatgcccgg aaattggggc ctgcaatgca
     3421 aggcctcgtg tagaagttgt actgccttgc tgtattggcg gtggggttag cactatcagg
     3481 tgcaatgacc cataggatag taaaccctgg gtgaatggta gttcctatta atgctcctgt
     3541 atttatagtc cgcaggcatg gtatttatta tgtcatttta tatggtttaa tcgggcttta
     3601 aattccataa aatcaatatg tgacgctaaa taaaaaatgc gtgcgcaggt ggtttatata
     3661 cacataataa ggaactacct gaacccggat aaattcatcc ggactgttgt ggaaattaaa
     3721 gagggtagat aaatgcgtaa tatttctgtg tctgcgccag ctcctgctgc gccattattt
     3781 cctgtatcga accatcatga gcgattttta cgcctgcctg aggtgatgca cttatgcggg
     3841 ttgtcccgtt cgaccgttta cgacctgatc agccgcgatg cgtttccgaa gcagatcccg
     3901 cttggcggta aaaatgtcgc ctgggcacag tctgaggtca gcgcatggat ggcggaccgc
     3961 atcagcgccc gtgggcgggg atgtgatgca tgatgatacc tgcatacatt aaataccttt
     4021 attctggctt gcttactgtc gtcatttcca ggtatagttt ttccgctgtc gcaaaatcgg
     4081 cagccggaat tggcgttccg tataacttat tggcgacaat tgacgcccct tgcgtctttt
     4141 tttacgtcgt agctcaggca caaccatttt cagggctgtg gtgtttatgc ttacaccatg
     4201 gttctatcga gataatggta gtccgggcgg ggcagccttc gggctggccg gtttccaata
     4261 aggccggtta cgccaacccc gttcgggctg ccaccagtga aattggcgtt tccggtggta
     4321 gcaataaccg ctacttattg gaggctgcca tcatggctac aatcctcacc ccgtcacacc
     4381 cgcaatatgt ctttgtgttt gccgcaatcc gccgtgctga cactcacccc cgcatctgta
     4441 tgcttcgcac tgtctcctgc gatgagcgtt ccgcgcgccg tttgctagtc cgagattatg
     4501 tactctccct ttctgcacgg ctgcctgccg gggaggtgac gctatgaacc agtttgagat
     4561 ttcctacgac gatgttgtaa ggctgaaaca tttacgcaat gtgggtgagt atgtaactgg
     4621 catggcagcc ctgcaggact gttatgaaaa gccagcaggt gcccaatgcg aacaactggt
     4681 ttccctcatt tatctgatga cagagcaact ggatggagtg gtgcaacgct gccatgacga
     4741 cctgatgaat gcggaggtgg cctgatgata tgctgtgaat ctcttctggc acttcgtgcc
     4801 gctctgtatc gccgtgccgt tgcctgtgcc tggctggctc taagcaatca tcaggaacgt
     4861 tattccggcc tgacgctcgc tgaacttgaa gatgccatag cccgcgagct ggaagggttc
     4921 tatctgcgcc agcatgggca gcaaagaggg ctggaaattg cctgcgcgtt gctttcggat
     4981 ctgatggagt cggggccgct taaagcctgt ccggttctct cactgctcgg aatgacggtc
     5041 atggatgaac tttgttcccg tcacctcaac aaaccagcgc tgcactaagg agggccgcac
     5101 aatgtcagga atgaaagtta gccaggctga gaaagcagct cggggtcact ggtcaagaat
     5161 tttacctgcg ctgggcgtaa atgtactgaa aaatcggcac cagccctgcc cggtctgtgc
     5221 cgggaaagac cgctttcgat ttgatgacca ggaagggcgg ggaacgtggt tctgtaacca
     5281 gtgcggggca ggtgatggcc tggcgcttgt aagtaaagta ctggatgtag gcattagtga
     5341 agcggcagac agaataaacg gcattatcgg aaacctgctg ccagtatctc agggaatgct
     5401 tgaatctggt tctcctgaaa aagaggacgg gagaaaagct gcagcagtgc tggctgcccg
     5461 tttgtttgat aagtcccgcc agaccactgg caatgcctat ctgacgagta aagggtttcc
     5521 tgcactgcct tgccgggaat taaccgctat gcataaagtc ggtggtgtgg catttcgcgc
     5581 gggagatctt gtcgttccat tgtatgcaga tggagagctg gtaaatctgc agttaatcaa
     5641 cgctaatggg ggcaaatgct tccttaaagg cggtcaggtt aagaatgcct tttacctggt
     5701 tgaaggtact gccaaagcag ccaaacggct ctggatagcg gaaggatatg ccaccgcact
     5761 tactatcaac tatctgactg gcgatgctgt catggtggcc ttttcgtccg tcaatttcct
     5821 ttccctggcg agcattgcct gcagtgagta cccaacgcac cagataatta ttgctgctga
     5881 ccgcgatctc aacggtgcgg ggcaaacaag gggcgcagct gttaccgggg cctgcaattg
     5941 cacaatggcg ctcccgcctg tgtttggtga ctggaacgat gcattcacgc aaaacggcga
     6001 agaagccacc cggcatgcaa ttcatgaagt aataaaacca gctgttgcca gccccttcga
     6061 cacaatgagc gaagctgaat ttaccgcgct gagcgtcagc gaaaaagcgc agagggtagt
     6121 ggatcactat aaaaattcac tggcagtaga cccgaacggg cagctccttt cacgctatga
     6181 ggcgggggcc tggaaagtta tctattacgc cgattttgcc cgtgatgtcg ctgcgctgtt
     6241 tcagcgcctc gacgcacctt tttcatccgc gaaaattgcg tctctcgtgg aaaccctcaa
     6301 actgatcgtt ccgcaacagc agaatccggc gcggcaactt atcggatttc gcaacggtgt
     6361 gctcgatacc cggacaggat tgttcagccc gcacgataag aagcactggt tacgtacgct
     6421 gtgcgaggtg gattacacgc agcccgttga cggtgagtca ctggaaaccc atgccccggc
     6481 attctggcgc tggctggatc gtgccgcagg ttttaatcct gaaaaacggg acattattct
     6541 ggctgcattg tttatggtgc tggctaaccg ttatgactgg cagctgtttc tggaggtcac
     6601 tggccctggc ggaagtggaa agagtattct tgctgaaata gcaaccatgc tggcgggtga
     6661 agataacgcg acctcggcaa ccattgaaat gcttgagtcg ccaagagaac gagctgcgtt
     6721 aataggtttt tcactgattc gacttcccga ccaggaaaag tggagcggtg acggggccgg
     6781 actaaaagcc atcactggcg gcgatgcggt atccgttgat cccaaatatc agaacgccta
     6841 ttcaacccac atcccggcgg tcatcctggc tgtgaacaat aatccgatgc gcttcactga
     6901 tcgtagtggt ggagtttcac gccgaagggt gatcctgcat ttccccgacc agatagcccc
     6961 ggaggaacgc gatacccagc tcaaagagaa aattgccagt gagctagcgg tgattgttcg
     7021 ccagcttatg cagcgtttca gcgacccaat gagtgccagg acattgcttc agtcacagca
     7081 gaactccgat gaagcgctca ccatcaaacg tgatgctgat tcagcttttg atttttgcgg
     7141 ctaccttgag gtcctacctg acaccacggg catgtttatg gggaacgcta atattgttcc
     7201 acgtcagcct cgaacttacc tctaccatgc ctatctggtc tacatggagg ctaacggcta
     7261 taaaaatacg ctcagtctga ccatgtttgg caaggggcta ccgttaatgc tcaaggaata
     7321 tgggctgcag tatgagaaac gacggaccaa tcaaggaatg cagactaatc tggccctaag
     7381 agaggaaagc aatgctgact ggttgccaaa atgcgatgag tttgcagcga aataactgac
     7441 ctaagaccgg
//
LOCUS       DABSYT020000163         7431 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.164, whole genome shotgun sequence.
ACCESSION   DABSYT020000163 DABSYT020000000
VERSION     DABSYT020000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.164"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..614)
                     /locus_tag="ID725_RS23050"
                     /old_locus_tag="pgaptmp_004610"
     CDS             complement(<1..614)
                     /locus_tag="ID725_RS23050"
                     /old_locus_tag="pgaptmp_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853060.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALS"
     gene            complement(635..904)
                     /locus_tag="ID725_RS23055"
                     /old_locus_tag="pgaptmp_004611"
     CDS             complement(635..904)
                     /locus_tag="ID725_RS23055"
                     /old_locus_tag="pgaptmp_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGV7853061.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(983..1621)
                     /locus_tag="ID725_RS23060"
                     /old_locus_tag="pgaptmp_004612"
     CDS             complement(983..1621)
                     /locus_tag="ID725_RS23060"
                     /old_locus_tag="pgaptmp_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGV7853062.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(1606..2838)
                     /locus_tag="ID725_RS23065"
                     /old_locus_tag="pgaptmp_004613"
     CDS             complement(1606..2838)
                     /locus_tag="ID725_RS23065"
                     /old_locus_tag="pgaptmp_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGV7853063.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRPLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(3339..5507)
                     /locus_tag="ID725_RS23070"
                     /old_locus_tag="pgaptmp_004614"
     CDS             complement(3339..5507)
                     /locus_tag="ID725_RS23070"
                     /old_locus_tag="pgaptmp_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGV7853064.1"
                     /translation="MIFPFGLNESQLLAVERAFSSQISVIEGPPGTGKTQTILNIVAN
                     ILIQNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVL
                     PDSPSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFS
                     HKYRFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSL
                     QLSYYEKNIREKQISLNEYEEAFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFS
                     AETYRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCA
                     RNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHY
                     RCHPKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHY
                     WDEESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQH
                     SRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFE
                     SPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHS
                     QIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQA
                     ERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(5504..6019)
                     /locus_tag="ID725_RS23075"
                     /old_locus_tag="pgaptmp_004615"
     CDS             complement(5504..6019)
                     /locus_tag="ID725_RS23075"
                     /old_locus_tag="pgaptmp_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853065.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKNVIRHGV"
     gene            complement(6260..7306)
                     /locus_tag="ID725_RS23080"
                     /old_locus_tag="pgaptmp_004616"
     CDS             complement(6260..7306)
                     /locus_tag="ID725_RS23080"
                     /old_locus_tag="pgaptmp_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGV7853066.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
BASE COUNT         2003 a         1901 c         1501 g         2026 t
ORIGIN      
        1 tcacttaaag ctctcttttg ttcatcatca tacagagatt gaatccggaa tttaatctct
       61 tctgcaacca gtcttgcatc gggctcataa aatatttttg ttattcgctg tttctgatgt
      121 tcaggctggt cttccatatt tcgtaaaacc agatgaaata atatatcata aagtgcgcaa
      181 tgccatgaat aaccatcatt ggttaaaata agctcccaaa cccgctcctg ctcggcaata
      241 agctgcagaa caaaggactc aacctgttct gtttgttcgg gggcaacacg gtatagcgga
      301 gcaatcacag caaaagatag ttcatttaac caggattccg cttccttctc gctcctgaac
      361 ggtggacagt tttgtattct tcgttcgtat tcacgggtaa tctgctccag aatatcctcc
      421 ggcggtgtca gccacttatt cctgagcaat acactggttg atggatgaca gctggctatt
      481 tcagcaataa tttcacgtaa tgtctccggg ctgaacttct tcagctgatt gtagtgctcc
      541 cgggtaaaac catgttcatc gcataaaagt tgccatgtca ttgatatact gtttgccatt
      601 ccttgcttca tcattaatac ctgttacctg aatatcaaca gaccggtgta ccttttctga
      661 atacctcccc ggacggagtg gcaatcccca ttcgcctcag cgtggcatct gcagctttcg
      721 ccacctcgct gatatccagc gtttcaggta gcggcgggga cgatatctgc gccagacaat
      781 aattccggcg caacaacaac cactcccgtt gtgcttctgt acattcaaca tttcccataa
      841 caaaaagcca gcggtcagaa gctctgcgcc agcaaccagc agcctccagt ttctgcgcaa
      901 ccatgtcttc cacaacttcc ggggtactct ttttcctgct caaaacatta actcactgaa
      961 ataatataca tatctgacgg tctcagcgaa ccgtccatgc cgggctgaaa tcctcttcct
     1021 gaaacagctc cgtcagcccg ctaatctgtt tcaatctgag aacttcatcc tggtccatac
     1081 ccagctccgt accaatgcgt tcattcgtcc agcccaggcg cgacaggtcc ctgacaatat
     1141 cagacattga ggttatctga tgtttgcccc tggcccggtt atgtcggatg gtcgcagcaa
     1201 tacgtgaggc ctgtcccttt ctctccggat taatacagac caccggcagc cagcccttca
     1261 gacgttttcc cgtacctgct tccctgccca gcacctggcg atgaaaacca tccaccacca
     1321 ggtaatggtt cttttcctca gacaccacca ccggttgtgt gaaaccatct ttctccagtg
     1381 actgcttcag cagtttcttc tcacccggcg ccatgacatt aggattgtaa tcattggcca
     1441 ccacctcatc tgcttttacc cacagcacac agtccactgg ctcatgtgca aaaggactga
     1501 cttcatgaag cactctgcgc aggtgattaa gggcctgtat ggtctcttct tctggtcgtt
     1561 cctggctgaa gtactgacga atcatgttgc caattatctc cggtgtcaca gaatccccca
     1621 ttccttcctc ctttctttca tacgctgcag ataacgttca tagtgccggg gtttgttcgg
     1681 actgaaggag aggggccggc accagaaatc attctttata agtgtcttac agatacgccg
     1741 ccaggacgga atatcccggc tccccaggtc attctcctgt tcatccggga tgtcatccgg
     1801 gaagccccgc gtctgatacc agcgcaggta gacagcaatt ttattacggt aatgttctgc
     1861 cgttctttcc ggcatcacat ccagcaggaa catcgcatag ctgcgccagg tatgatgggc
     1921 tggttttgat atacgtttac gcagggcaaa ataggcaccg ctttcattgg cataaagcgc
     1981 cccgctggca gcgcctgaca cccgctcaca catcctggcc caggtttccg gctccagaac
     2041 atggtaaagc cacagcccct tacgttgttc aggaccaaaa ggttcacaga cacgcatgtt
     2101 gcgtaacggc acgtcggcac ggtacatcag gtcatacaga ggattgtaaa tggcacaagc
     2161 tctggcgtta tatatccaga tatcgcgggc tttccagtca tacaacggat acatggtgta
     2221 ataaaaccct tcaggcgatg ccgtggtcca gggcttatca tcggcatatc tcagtttgcg
     2281 ctgagacacc agccccataa agcgattgag ggattcatcc gcacgaacac cggtcagcac
     2341 tgccactcca caccggttac cggcaaacca ggaagaaaat gccggaacaa attcttcaaa
     2401 cgtcatggca taccggaaaa agggaaaata tgtcatatcc gtaatggcct cttccggtgg
     2461 ctggcgaacc caggtcactc ctggctccca gcatatccac tccggctgaa actgcgagac
     2521 gccgtttacc gtggtcaggg gaagtgccac ccagtaaaag gtttccgtca catcatggta
     2581 catttcccgc atcttctgaa tatgttcaat ggtgcaccga tactgggcct cccagtcaat
     2641 gaacagaaca gagaaatgac gttttctcct gcgggccacc tctgccacaa gatggaaaag
     2701 cacggtggaa tcctttccac ctgaaaaaga cagacatacc gacggaaatg tttcaaaaat
     2761 ccactcaatg cgggcgattg cagcacgcaa aacattttct ccggtaagga ttttttcggt
     2821 taaaagcgta tcactcattt tcctggcttt atgtcgaata attctgttta tacattcagg
     2881 caaacttcat taataactca ccgctttggg gataaataat aacaacaaaa atcttcagat
     2941 attaacctgg ttgttggatt tgttatcaca tcctttatcc acctgcttct tactggtaac
     3001 acacctggaa aatccatgca ttttcaacat atactatcct ttcaacttgt aaataagaaa
     3061 atatgatgca gatggtgaac aattcaaata tcattttgca ggaagtattc actgtaatag
     3121 aattaccccc tgataaagtt tattcactaa ggaattattc aacagaaaca ttccctgtta
     3181 gctttgagca atagcactta tttttatatg tatcaggaat acgtacgttg atgtaaccaa
     3241 aacaaagaag cctgtgtcca tggggttcgt acaattgtgt gtggcacatg accaaatcag
     3301 gtttgagacc tcccgctgca atatttttta ttcagatttt agcctgtcag gcctgacaga
     3361 aacgctccca acttcccttc tatatcactg tctatagtac gtaatcttag caatggcagg
     3421 ccgcatttct tcagtatact gtttttaagt tcatcacgtt cagcctgaac actggtcaga
     3481 tgataacctc catccacttc aataacccct aatggagtct tcccgacctt atagtaaaca
     3541 acaaagtcac aactggcccg gttacgcatg aataactgtt cacggtgtgt aaaatccccc
     3601 cgctccagca acaccaactg attcagcgct atttgtgaat gaaacatcat actccgataa
     3661 ctatcctgcg acaggatatc ccgcaacagg caggccacaa tctgttctga cttaaagtgt
     3721 gaatcatttg aattcaatct actattcagt cgttccagtg atttgtcata ctcactgtac
     3781 agcaaatcaa atgctgatat gaccgggctc tcgaaaatct ctccgtcatc agcgtagtat
     3841 ttgatgtaac gaatcagcgc cgcaatatgt ccagcgtggc gttcaaatac atcattacca
     3901 gtcaccagcg taaatttatt tcgggccctt gaaacagcga cattcaccag ttcaggatta
     3961 tcaacaaacg cgatgttccg actgtgctgg ctgctgcgct tcttatccag cacagtggaa
     4021 aacacaattt catcgcactc ccgtccctga aatttatgca ccgtcgattt cacaaaatca
     4081 gcaggcagca ctttttcagc cagattaacc tgtgcattat atggggcaat gtatcccctg
     4141 ctactttctt catcccagta atgaccttcc agagattcaa gctccctgag atttgaaaaa
     4201 ttacgggtat ggtttccttt tgccgtgatg accagagata atgaggcttc accggaatca
     4261 accgttaacg gtatcagagc attatcataa aactgcttat tacaaaattg aatgatttta
     4321 ggatgacaac gataatgctc tttaagaagg gttactggca ccatgttcct gaacaacata
     4381 caaaccgaat caagtagact atatttctca caattatagt attctgccgg tggagatggg
     4441 ctgttcggga gtaatacagg aacatgtgga agttgcttac gatcccctac aacgattaca
     4501 ttacgggcac atcccagtcc cagaatgccc ggaacaatat cctgctgcga ggcttcatca
     4561 ataataacat aatcaagaag ggcccccttg ccgatagagt taataatcga atgcgtactg
     4621 ctaccaataa tcggaaaacg ctttataaaa cggtcaaatt catccctgta tgtctctgcc
     4681 gagaatgaac ttcttgttga aacatttctt cgcagatgtt gctttaaata aagcatagac
     4741 catgatgtca ggcgccccag taaaatttta aaatcagatt ttttaaatgc ttcctcatat
     4801 tcattcagag aaatttgttt ttcccggata tttttttcat aataagaaag ctgaagagaa
     4861 gtaaatagcg ccagccttct ctccggaatc atcaatggtt tgactttcag tatcatgaag
     4921 tttaacaaca aatcaattct gtttctgaat ccaatccgcc tgtcagaaag gtaatgaata
     4981 taagccatca ggtcggttgt tttttgtgag gaaaatcggt atttgtgaga aaaaagctcc
     5041 tccggtcgaa gattctgact ctgccattgc tgcaggtatt tatactcaat attcagctca
     5101 ttaatttctg cttttaacga agcgacctgg ttaatggcat tcaggtgttt tttcacttgt
     5161 tgaagtacat catcaatcgc atttgcagac ggtgagtcag gtaagacttc ctcagaacgc
     5221 gaaatcgacg ttgaaaaaaa ctgttgcctg ttctccgtac tccccagtcg tgccatcaca
     5281 tatcccagtt gctgtttgtc catcttttca taaacattac tgacagctga gttattattg
     5341 gacaaaattg ccactgtttt attctgaatt aatatattag caacaatatt gagtatagtc
     5401 tgtgttttac ctgttccagg tggtccttca ataactgaaa tctgtgagga aaatgcccgt
     5461 tcgacagcca gcaactgact ttcattaaga ccaaacggaa aaatcaaacc ccatggcgaa
     5521 tcacgttttt actctgtcct gaacagtatg catgcaaggc ggtatccgga tgaggaacta
     5581 ttttctttat ctgcctaagg atattctcat cgatatttct gttattacca cgggcataaa
     5641 aaatacgttc attagccacc ctacacaagt aattaaacac ggcatgctct gtaaatgctg
     5701 aacactgttg aaagtcaagg ccggtcgttt tcatgatgta gggtcgttca ccctcgcggt
     5761 aataaacagc agcatattta tcaccatata taataaccag ttcagcccgg gatgttacag
     5821 cgttcccttt cctttcgagg aaaacatttc gcaaatttac agttggcgtt atctgacata
     5881 cagaaagcgg acgagtatat tttctcccgc tgcgaaaatg acaggtcagc ataacctctc
     5941 tgtcgtcatt tccagcccac agcgtccagt cggtaatttt atgagtctta ttttcaccgt
     6001 caacacaaat cgtgaccata ttcttactca gaaattatca tttaacttac ggaaaattgt
     6061 taaggcactc ccgatattag ctccggagga ttttccacaa ctccgggact gttaacatat
     6121 ttctgcctgc catatataca ttgaatttag tcataacagt caataccaga attacaggaa
     6181 tgcgatgccg atacgctggt tattgaatac tggtagcccc tgagcactaa ttcatcacga
     6241 tactctgtat ttactgtttt cactgctttt tacaaccatt cccccaggta aaatgctgct
     6301 cctccggtga tggctgtgtc agaaactgcc atcccggaat agccacgctg accgccagca
     6361 atgccgcaca gacaccagcc acaaacggtt tcagaaacgt gggcggggat ttctgaaggg
     6421 agggtgactg ctccgcctgc actttcccct tgcgattatc aaggctcagt gcatccagac
     6481 gcaccgcgcc atttgccgca agcagccctt cccggttatc cacgcttccc ggcagactca
     6541 gcgaaaaatc ccctgttccc gtctgaatca gactgccttt acgatttgac aggctttgtg
     6601 ccgatatctg cagtgtgtcg gcatttatcg taccgccatt actgctgaac tgcccggcag
     6661 tccgggcgga aagcgtatcg gcactcagtt gcgcattctg tgtagacaga tttccactgg
     6721 ttgcattcag ctcagtatgt tgtccctgta tccggctgtt acctaaatcc agcccctgct
     6781 caatgtcccg taaatccagc agagaacctt cgtgtgccgg gaatgaggaa tacaactgaa
     6841 tatagtcatc actgtccggt gtcagacgca catgtcgcac aacaccggga ataataacca
     6901 gccggaggat accgcttttt aaatcctgcg aaggtgccag aacacgggtg gtgacataac
     6961 catgatcgac cagacggttc tgcaatgtac tcatcagcag attaattcct ttcgccccca
     7021 ggcaatgccc gaccgccccg ttggcgattt tttgtaaagg cagccagtgt ggtaacgcat
     7081 cagccccctt cagttccgtc tgtttaatct gaaaacaggg cgtttcaaca ggaaaattaa
     7141 tcttatgggc aaaagagcca ggtgcagaaa ggcgaacatc aggggctgaa ggcgtcagtt
     7201 gttgctcaag ggcttcctga cgctgctgct gaatgataaa tgcttcatct ccgctacgcc
     7261 ggacatccgc tgcctgaaga gataaaggcg ataataaagc agacatcagt gtcagtgaca
     7321 gcaaggacgg acagcgccgg aataagcttt gttcctcagt cctctcgttc ctgcaacata
     7381 attttccagt caaacgaaaa accttttatt catgtcatac caacaatcac c
//
LOCUS       DABSYT020000164         7357 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.165, whole genome shotgun sequence.
ACCESSION   DABSYT020000164 DABSYT020000000
VERSION     DABSYT020000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.165"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..460)
                     /locus_tag="ID725_RS23085"
                     /old_locus_tag="pgaptmp_004617"
     CDS             complement(<1..460)
                     /locus_tag="ID725_RS23085"
                     /old_locus_tag="pgaptmp_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853067.1"
                     /translation="MNKNLLTRVIIISDDLYYLQGLSAKLGHHYRFFIECFFCSQNAC
                     NNDLSTLRFDNRFTKNVLLVAVDDVSILAKLPDLARFLMIVSLRKMKNRNIFFSLSEQ
                     TFISRYIKLNKLITLIKSTDKDQQVRQVTLTRYEWDIYYLYFKIDNKRILH"
     gene            1851..5663
                     /locus_tag="ID725_RS23090"
                     /old_locus_tag="pgaptmp_004618"
     CDS             1851..5663
                     /locus_tag="ID725_RS23090"
                     /old_locus_tag="pgaptmp_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HGV7853068.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINARGSIENRDGNNFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNSL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            5752..>7357
                     /locus_tag="ID725_RS23095"
                     /old_locus_tag="pgaptmp_004619"
     CDS             5752..>7357
                     /locus_tag="ID725_RS23095"
                     /old_locus_tag="pgaptmp_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGV7853069.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTSVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGISEYLSAKATHTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIENGYYNTAFFAQYAPRELTLQDKITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGSAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNF"
BASE COUNT         2163 a         1465 c         1751 g         1978 t
ORIGIN      
        1 tgtgcaatat tcttttgtta tctatcttaa aatataagta atagatatcc cactcatacc
       61 ttgtcagagt cacctgcctg acttgctggt ctttatcagt acttttaatc aaagtaatga
      121 gtttattcaa ttttatatat cggctaatga atgtttgctc tgaaagtgaa aagaagatat
      181 ttctgttttt catctttctt agtgaaacaa tcattaaaaa cctggccaaa tctggcagtt
      241 ttgcaagtat acttacgtca tccactgcga caagaagtac gttttttgta aagcggttgt
      301 caaatctaag tgtacttagg tcattattgc atgcattttg agaacaaaaa aaacactcaa
      361 taaaaaaacg ataatggtga cctagttttg cacttaatcc ctgaaggtaa tatagatcat
      421 ccgaaatgat aattaccctt gttagtaagt tcttgttcat tgcactaact caattgcttg
      481 cagcaaaaca gcccatcttc atggagtaaa aattttgaaa gtgaataaag ctttttacta
      541 aagatgtaac aatgggcatt ccatatgcga cataacaact gcagtaacac aactaatctt
      601 tgcgtgttct ttatattaga gagtaacagc gtgatatctg tatctctaaa agaatagcga
      661 tgtcctgcaa tcgatgagca caatatatga cattcatcac atgtataaca attgaatttt
      721 cattcatact aatgttgaac ggtaatatag taatttaatg cttgccgaaa gatgataaac
      781 agagttttat tctgaataag agtgtttttt gcgattttct caggttgtaa attctgcata
      841 atggctgaac ctattacttt ttatgtcaaa aaagccgttt ttataagaat aaaacaacga
      901 tatttaagag tgctggcagc ggagaaaagg gttaatacag tcatttaaga ttacttcagc
      961 ttttcctgtg gtgagaaatg agcggaattg tagcgcaatc tgtaaaaaca attcctctta
     1021 actaataaaa atcgcgaata atcattgtgt tagattgatt tgttgcaaaa taataacaac
     1081 attattaaaa tcattacaat ctttaattta gaataacatt tttatgaaga ccagaatacg
     1141 ttaagggatg atgtatagcg gtgattgcta ttagatggga atgaaagctt gccaaaccgt
     1201 tttgctcttc tttctccctt tcaacgataa gccaggcata atgtggctgc ttaagccaat
     1261 tgtcgcaaag ccaaagctct ctatatagct aacctcatgg ttaatccgat ctcagtaata
     1321 ccatgagcaa gaactctttc ctttttatat aactcttaag ggttacatgc gtgaatgcaa
     1381 atggtaatat aatgaaatgt cgcctgcccg taatgaccat aaagaatcta ccccgacgta
     1441 aaagatatag agaataagat gcagtaatga tgatgctttg ttaatcaaat aataagattt
     1501 aaattaattg taactcacca actttgttgt tttattcgga tgtctgaaat gatgtcgtac
     1561 agaaaactac atatcatttc agacgtcctc taaattggat ttttttcacc caaggagcaa
     1621 ggatggggga cttttaaatt attgtgtttt agtaagttag gttcttcgcc gttcgatgaa
     1681 aaaacaataa ctcttagttg tttttttgag tccttatttc agcttatttt tcgtgatttt
     1741 ttattgcgcc aggtgatagg atttgccgca atcatttttg ctgatattcc gtgagtcatc
     1801 aaaagtgatc tgcatgtcca aaatgaatag catcaataag gaaaacgtta gtggcgatga
     1861 taaatttaag taaggaagca acggtgggga aagcattaac ccctattgct atacttatga
     1921 tgttgtcttt tcctgtagct tctcaagcgg cgggattagt cataaaaaat ggaacggtat
     1981 ataacgccaa tggtgtgcca gtcgttgaca tcaacaaacc taacggtagc ggtttatctc
     2041 ataatatctg ggataaccta aacgttgata aaaatggtgt cgttttcaat aatagcgcta
     2101 atgaatccag tacttcactt gccggaaata ttcagggaaa cagtaatctg acctccgggt
     2161 cggcgaaggt gatcctgaat gaggttactt ccaaaaatcc ttcaaccatt aatgggatga
     2221 tggaagttgc aggggataaa gcggatctga ttattgccaa cccgaatggt attactgtaa
     2281 acggtggcgg ttcaatcaat acaggtaaac ttaccttaac caccgggacg ccggatatcc
     2341 aggatgacaa gctggccggt tactccgtga acggcggtac cattacgctc ggtaaactgg
     2401 ataacgccag cccgacagaa attctgtccc gtaacgtggt agttaacggc aaagtgtctg
     2461 ccgatgagct gaacgttgtt gctggcaata actatgttaa tgccgcaggc caggtgaccg
     2521 gtagcgtatc cgccacgggg tcccgtaacg gttacagcgt agatgttgcc aaactgggcg
     2581 gaatgtatgc gaacaaaatc agtctggtca gcaccgagaa aggtgtgggg gttcgcaacc
     2641 tcggcgttat tgctgggggt gttaatggtg tcagcatcga ttccaaaggt aacctgttaa
     2701 acagtaacgc ccagattcag tctgcaagca cgatcaacct gacaacaaat ggtactctgg
     2761 ataacaccac cggtacggtg acatctgtag gcactatctc gcttaatacc aacaagaata
     2821 ctatcgtgaa tacccgtgcg ggtaacatct ctacgatggg cgatatctac gttaacagcg
     2881 gtacgattga caatactaac ggcaagcttg cggctgcagg aatgctggcg gttgatacca
     2941 ataacgccac gctgattaac tctggtaaag ggagttctgt cgggattgaa gcggggctcg
     3001 tggcgctgaa aaccggaacg ctcaacaaca gcaatggtca gattcgcggt ggctatgtgg
     3061 gtcttgaatc cgctgcgctg aataacaaca acggtgatat ccagaccacc ggcgatatcg
     3121 ccattatcag taacggtaat gtggataaca acaaaggtct gatccgttcg tccaccgggc
     3181 atatcgttat tggcgcggca ggtagcgtaa ataatggttc aaccaaaacc gccgataccg
     3241 gcagttctga ctctctgggc attattgcag ataccggcgt agaaattggt gcgaacaaca
     3301 tcaataacaa cggcggacag attgcgtcta atggcaacgt ctccctgtca agttacagca
     3361 cgatcgacga ctatgcgggc aaaattctgt ccaacagcaa agtgattatc aagggaagct
     3421 ctctgcgtaa cgataccggg gggatcagcg gtaagcaggg tattgaagtc gccgttggcg
     3481 gcagcctgac caataatatt ggcgtgatca gctctgaaga gggtgatatc tccctgttag
     3541 ccaactccgt ggataaccac ggcggcttca tgatggggca gaacatcacg atggagtcga
     3601 tgtctggcgt caataacaac acagcgctga tcgtggccag caaaaaactg aagataaatg
     3661 cgcgcggcag tatcgaaaac cgcgatggca ataacttcgg taatgcttat ggtctgtact
     3721 tcggcatgcc tcagcaaacg ggtggaatgg tcggcaagga aggcatcgag ctttccgggc
     3781 agaacatcta taacaacaac agccgtctta tcgctgagga tggtcctctg actctgcagg
     3841 cgcagaacac gttcgacaac acgcgtgctc tggtcaccag cggggcggat gcatctattc
     3901 aggttggcgg aacgtattat aacaactacg ctaccacctg gagtgcgggc aacctggata
     3961 tcgacgcgac cacgctgcaa aacagcagca gcggtacgat gatcgataac aatgcgaccg
     4021 ggttcatagc atctgataaa aacctgtcac tggaagtggt gaatagcctt accaactacg
     4081 gctggatcag cggtaaaggc gatgttgatg tcacggtgaa taacggcaac ctgtataacc
     4141 gcaataccat tgcggctgaa aaggggctgg atattgccgc gttgaacggt attgaaaact
     4201 ggaaggatat ttctgctggc ggcgacctga cgatgaacac caatcgccat gtgaccaaca
     4261 actccaacag caatatggtg gggcagaata ttgttattaa cgcggttaac gatatcaaca
     4321 accgtggcaa cattgtcagt gacgctgacc tgaacgtgac gaccaaaggc aacctgtata
     4381 actatctcta tatggtaggg tatggggata tcgcattgtc ggcaaatagc gtggcgaaca
     4441 ataacgcgac catcgaagcg acaggcgatc tgattatcga ttcgaagggt aacgtgggta
     4501 acaaccgcgg taatctgcat gcgttgaacg gcgtgttgtc tgttaaaggc aacaatctga
     4561 acaacgataa cggtgaaatt cgtggttatg gcgatgtcac gctggcactg acgggcaact
     4621 acgacagcta taagggttcg ctgacctctg aaacgggcga cgtgactctg acggcgaaca
     4681 ttgtagacaa cgcctatggt ttgattgccg gtgagaatgt ttctgtcgat gctaaatcga
     4741 cgatttacaa caacactgcg ctgatcgcgg cgaataaaaa gctggttatt aacgctggcg
     4801 gcaacctcga aaaccgcgac gggaataact tcctgcgtaa taacggcgcg ctgtttggaa
     4861 ttaccgacaa cgttggcggc atcgtaggta aagaaggtgt cacgctttct gctcagaacg
     4921 tctacaacaa taacagcagc atcatcgctg aaaatggtcc gcttaatctg ctgtccaggg
     4981 gaacgctgga taatacccgc gcgcttctta gcagtggggc tgatgccatc atccgtgcgg
     5041 cagggacgtt ctacaacaac tatgccacca cgtacagcgc cggtaatctc gacgtttatg
     5101 cggcgtcgtt gaacaacgcc agcgatggtc gcctggaaga caataccgcc acgggcgtga
     5161 ttgcgtctga caaaaacctg gatctgagcg ttgataacag tgtcactaac tatggttgga
     5221 tcagcggtaa aggagatgtg catttcaatg ttctgaaagg cacgctgtat aaccgtaatg
     5281 ccatcgcggc ggacaacgcg ctgaccatta atgccctgaa cggtgttgag aactttaaag
     5341 acattgtggc gggtactgcg ctgactattg atacgcagaa gtatgttacc aacaacagca
     5401 acagtaatat gttgggacaa accatcgcga tcaatgccgt gaatgacatt aataaccgtg
     5461 gaaatattgt gggtgattat tctctgggtg ttaaaaccac cggtaatatt tataactacc
     5521 tcaatatgct gagttatggt gtcgctggcg tatcggcaaa taaggttacg aatagcggta
     5581 aagacgctgt tctcggtggc ttctacggtt tagcgttaga agcaaacgaa actgataaca
     5641 ccggtactat tgtcggcatg taagatgtaa ccaaagacag cagtggcttt taatattaat
     5701 gcgtcatgtc acgcaggggt tgctgctgtc tttatttagt ttgagaagat aatgcaatta
     5761 cgatttaatt ttggtttgct gctcattctt tgttgttttc aggttgcagc gaaagacagt
     5821 cttgataatt tgatcaagca gcaaaaaaat gcagatgaaa gcacgttgcg cgcgcataaa
     5881 ttagaaaaaa aagatgttta ctcaaatgca caacgtaatg cgtttactct gggtgatctg
     5941 ccgcaggaaa agaattgctt tatcattaat cggattgaac tggaagataa ctttctgggt
     6001 ggtcagttga tttctgaagt gcaaaaagcg attgccggac gttgtgtcgg tagtgctggt
     6061 gtgcaaaaaa ttgcagctgc cttgcaggat tattatatta atgcgggata tatcacaacg
     6121 cgaattagta tcccttcgca ggatatttct aaaggcaagc tacgtttcca ggttaatgcc
     6181 gggaagatag aaaaaacgat tgtcgaaggt aatgatatca gtgaatggat gttacctttt
     6241 cagcgtaacg atattctcaa tattcgtgat attgaacagg ggctggaaaa tcttcagcga
     6301 gtacctgacg ttgatgtgaa aattaatatt gaaccgggaa cacgggatgg ttacagcatc
     6361 gttcatattg atacacaccg ggggaaaaac tggagcgttc gggcaagtta caacaattgg
     6421 ggcgatgaag aaacgggccg ttaccagaca acctcggtgg gttatctgtt taatccagcg
     6481 aaaatggggg atctgtttta tctcgccggt acgcgtagta ctaccggaca gtatgaaaac
     6541 gtcagtagtt attattccat tccggtgggg tactgggagt acggcttttt ctatagcaaa
     6601 agtaaatcgc agcaggtgat tccgctttcg tacatctcgc tggattatgt tggcatcagc
     6661 gaatacttga gcgcaaaagc gacgcatacc gtttatcgcg ataaaacaaa aaagttcgcc
     6721 ggtagtgcag aactcattcg acgaaagtcg aattacacgc ttaatggtga ggagctgaca
     6781 ttacaggcgc gggatatggg aaatatcaag ctggggctaa attataagca acagttaccc
     6841 ggcgcgttct gggattcaac cctctcgtgg caacgctttt taacctggtt cggtggtgaa
     6901 aaaacgccgg atatgcaata tggcgacgtt agcccggtaa gcaatatctt taattttgag
     6961 ggtaactata cccgacaaat agagaatgga tattacaata ccgctttttt tgcccagtac
     7021 gcaccgcgtg aactgacttt gcaagataaa attacagttg gcgatcgttg gagtattcgc
     7081 gggtttgaaa atagcgtagg tttgtcaggg aatgatggtt tttatataaa aaatacgctg
     7141 gcatttcctt tgcctggaat gaaagctaat tattatgccg gtcttgattt tgggcaagtg
     7201 tatcaggacg cttcttatgg cgatgaatca ctggtgggct cggcagttgg tattgatgga
     7261 aacattaaaa gtcttgagta taacttttcc gtttctaccc cattaaagta tccggcgaca
     7321 ttagatattg atcgggtgaa tgtaaatttt aattttt
//
LOCUS       DABSYT020000165         7303 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.166, whole genome shotgun sequence.
ACCESSION   DABSYT020000165 DABSYT020000000
VERSION     DABSYT020000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.166"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(173..379)
                     /locus_tag="ID725_RS23100"
                     /old_locus_tag="pgaptmp_004620"
     CDS             complement(173..379)
                     /locus_tag="ID725_RS23100"
                     /old_locus_tag="pgaptmp_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7853070.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
     gene            complement(479..1330)
                     /locus_tag="ID725_RS23105"
                     /old_locus_tag="pgaptmp_004621"
     CDS             complement(479..1330)
                     /locus_tag="ID725_RS23105"
                     /old_locus_tag="pgaptmp_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HGV7853071.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1757..6343)
                     /gene="ypjA"
                     /locus_tag="ID725_RS23110"
                     /old_locus_tag="pgaptmp_004622"
     CDS             complement(1757..6343)
                     /gene="ypjA"
                     /locus_tag="ID725_RS23110"
                     /old_locus_tag="pgaptmp_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HGV7853072.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYH
                     SNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELG
                     ATLSYNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKA
                     SFSSTLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(6408..6527)
                     /locus_tag="ID725_RS23115"
                     /old_locus_tag="pgaptmp_004623"
     CDS             complement(6408..6527)
                     /locus_tag="ID725_RS23115"
                     /old_locus_tag="pgaptmp_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGV7853073.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6751..6909)
                     /locus_tag="ID725_RS23120"
                     /old_locus_tag="pgaptmp_004624"
                     /pseudo
     CDS             complement(6751..6909)
                     /locus_tag="ID725_RS23120"
                     /old_locus_tag="pgaptmp_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1811 a         1823 c         1573 g         2096 t
ORIGIN      
        1 cggctagtgg gctgtatcca tacccgatat taagaaaacc atctctaagc cttatagcaa
       61 aagctctgcc agaaaaaacg taaagaatta cgttaaatat agattttttt aagatttaaa
      121 ctttacaaat ctgagaatta atcctcataa ataagaagac tgtcatttta tttcaggcaa
      181 catgctttct tgaattttcg atagttaatt gaatccattc gtctacttca ctttcaatga
      241 aagcaacaga gcgcgcaccg attttaacgg gggaaggaaa acgattttgg ttgatgaggt
      301 gatagatcca agctttaccg tatccagttc gatgcagaac ctctggtaaa cgaataaggc
      361 gggtgttgtg ctgattcata gtcgtttcct ctgtgccgtt ttgatggaaa tgactataca
      421 aatgaatcag caggaaaaag accgaagctg ggaacgccag agctaatgga ggttggtctt
      481 agtcagaatc agcgattttc atcacgtctg aaactctgat ctttttcaaa tggctgttct
      541 tgttgatgaa cagattaaac tggcgaataa aaggtatact cgccgccttc aaagcaagag
      601 gtttgtctgt atccctgatt tggtggttca ggcgagtgtt gatctcatca tcgtcatctg
      661 tatacaacag gcgagaaagg cggtcttctg atatacgtac tttatataat tttgaccata
      721 ccagaatgtc gatcatggga aggagtctgt aattaatgat tttcttaatc gttccataac
      781 caaagcgaat agcttccgtt acatctggtt ctatgccacg cactttacgc cattgaggaa
      841 gtgcagcctt tagggattca gcgatctctt catctgtccc gtttgctagg tcgatttcga
      901 ccatgacagt atccctgaac aattttggca aaacttccga tacagggcta tcgaggaatt
      961 ctctgttaac agaatactca tcgcttccat cccaaaaaaa cagtgagttc cgtaatccaa
     1021 ctatgctgag ttgagccagt ctttcggttg tcgtcaaaaa gaagtgcggg ggctggtaaa
     1081 gagtatcctc tcttgtgaga tagccgagat gcttttctgt gatgagaaag ggctttccag
     1141 tgaagatcct gtcgatgtag agccttacgg cttctgcttc aaattcttca tgatatggtt
     1201 tgaagaacaa tgtccttgcc agcaattcat ggtagaggtg tataagcgga agctcttcga
     1261 atccgcgata tgtatctata tccagccact tcttcacctc tctcgtatgt tcaactgacc
     1321 agtttttcat aacgatctcc aacaggcatt agacttaatg tatgtagcaa acgtaatcga
     1381 catgttaaag gaagaccaat gaaatgagtt cagatgccat caggaagttg aaccaaaagg
     1441 tttgatattt tttgatacag ttcgctaacg gactattttc aggggtttat gccgttacga
     1501 tttatggttc ctttatagaa ccaaaggtaa agtcagaaac agcagttatc tagaaaattc
     1561 aatcaaatag atatctagtt acagggacgc cagccaatca tgattggacg gtgtaaggac
     1621 aacaccaaca aaaacaggaa gttagaagtc tcagcaggac accgaccaga cggtgaggag
     1681 acataaaagg atacgcaaag gagccgcggc tcctggtgac atgaaagccc acagatgtgg
     1741 gctttttcgt tgatggtcag aacgaccagt tcacaccagc taccgcgttc cacggggatt
     1801 ccacaccggc accatggcta taccccaccc caagatgccc gcttaacgta ctgctgaatg
     1861 aggctttaat acctgcctgg tatattccac gtctgcccga caaatcattg acgaaattac
     1921 cgtcattatt caccttcacc cggttatcat cgacaaattc tttgcgcaca gccgccttca
     1981 gccacggctc aatttccata ccgttcccca gacgcatgtt gtaactcagc gttgcgccca
     2041 gttcacgata tatactgcgg gtatcgactg atttcgattc catgccattg gataaatgat
     2101 attcggggtt atcagcggtg aaccccgtta acgatgcata cggcgtcagg ttccagttac
     2161 catcggtaaa tcgcatcccg gtttcaatgt gaccgcccag cccgttgctg tggtaactgc
     2221 cattggcggc tccaccgctg ctcattttac cggctacgtt actttcaaaa cggttcagct
     2281 tcacgacacc gtccagatag aaaccacttt catgttccca actggcatag ccgcccagag
     2341 aataactgcc cacactgcca tgtcctccgc gatcaaaacc gatatgtgaa tgggaataac
     2401 ccataaaagc gcccagcgtc gcaatcccct caggaatatc attagggctg tcgatcccca
     2461 ctgtcattcc ggtcagcgtc tgctcaaacc cggcccccgc atcggtggtg acattattac
     2521 gggtgttata cgtcgccccc cagacattat tgttgtgtgg actcgctttc attatgttca
     2581 accgctcgcg aatactgttt agctcagcat caaataccaa cggtaatgtt gctgccatat
     2641 tgagtacggc tgcggtagaa ggcgtgatgc gtttctccgg aacgggtgtc ggcgttggct
     2701 cgggagtcgg gtctggtttc ggatccggtt ttgggtctgg ttttggatcc ggcttcggat
     2761 ttgggttggg atttgggttg ggatccgggt tgggtttgac atcattggtc aggttccagt
     2821 tgctgttgcc atcgcttttc aggacatact cataggtccc aagatcaacg aaaccgccag
     2881 tattgcccag cgaaaacgaa gcatcccctc cccctgtttt caccagcgtc atcgcgtcgt
     2941 cagactgagg actgacgccg gtatcctgaa caaagatttt aaaattacca gtggcgttgt
     3001 tgttgacgac cagttgatcg ccccgagagc ctgcaacgtt ggtatgcagg tagaaattac
     3061 cgctaccgga aagttcattg gttgtcagcg tattgtatat accgttagtt gtggcaggcg
     3121 ctgctgtttg tgctgataaa taaacattac cgtcgttaag tagcaaactg tttactctat
     3181 actcgcagtt gctggtgcct gcacaggaat tattgctgtt aagccagaca ctgccccggc
     3241 tggcagccgt caggtcggca agcgtcgtat caacgccatt gccgatagtt aatgtcgcgc
     3301 tatcggtaat ccggatcgcc ccttcgagtt taactggcgt aacattatcc cgtggcgtca
     3361 ttaacgacag gcttcctgtc gcgccactga ccgatgcatc cgccagcgta cctgcgtaga
     3421 cgattgctgt cccgccagca acctggagat cttctgcccg ggccagagcc tgaaactcat
     3481 cttttgaccg cccaagggta tattgcccac cagagttaac ctttgtggcg gagtcctgac
     3541 cctggttttg cattgccccc cccttgccaa ccgtggagtc gcgagcttcg gtacctgcta
     3601 ataccaataa attaccgcca ttttccagca acatattggt cgctaaattg ccggaaatgg
     3661 aaaaagtgcc gtactggtga gtaccgctga tttcaatacc gttagccgtg ctcgtctgga
     3721 gagcggcacc gctgttctgg acgatatctg tcgctttgcc attatcgttg actgtcagcg
     3781 taccgccttc attgatcgtt gtttttattg cctctccgtt agctgaaact gtttgtattc
     3841 cgccgtcgtt aattgttgtc tcattcgcca caccctcgac aatttgttca ccgccggtga
     3901 gcgtcgtgcc tgtcgcagtg gcttttgttt tgacgatctc ccgtccgccc gtattgacct
     3961 gtgttttgtc agaagaggtg tctgactcca cggttaacac tccgccattt gccagcagga
     4021 tattgttcgc cgcaccctgc tcgatgctga acgcgacgcc atccgcgcgt gttcctgtga
     4081 cccgcgtcgc cctggtggtt gcaaccaacg cgccctggct actctgctgt atccccgttg
     4141 cgctgccttt ctcccgcaca tcgagtgtgc cgccatcatt aagcaccgag ttttcagcca
     4201 gaccgccctc attaactatc tgtgaaccgc cattaataat ggaaccttcc gctgtcccgt
     4261 ttgccataat ttgttgtagg ccagagacga tatcggtatt gatcgcctta ccataattct
     4321 gaacggtttg cgtgccacca ttgatgtgtg ttttctctgt tgacccacca tcaacaattt
     4381 gttcaccact ttcgatattt gcttcagtgg ctaaaccata taccgtttgc ttgccacctt
     4441 tgatatttgc tttatcagaa gtggcactgg catatattgt ttgggtgcca gcactattga
     4501 gtacagtgcc aacatctttt ccataaacat ccattttgcc gttggcatta ataatcgtgt
     4561 caactgcccg ggaaccagtg acgactgtta atgagccagc gttttccagc actacatttt
     4621 tagcttctga attcctgatg tagaaagcat caccataact gttggttcct tcgataagtg
     4681 ttccggaggt cgtggaagca attaatgcgc cgccggattg ttgctcaaca tgcttagcct
     4741 caccaccgtc ctgaacctcc agaacgccgc cattattaag tctggtgcta tctgttttag
     4801 cctccttctg gacaatcagc ttaccgccag catcaatggt agtatttttc gccgaggttt
     4861 tagccactac cgtcagttcg ccggtatttt ccagcacaac ataattagcc tcccctccgg
     4921 taatagtgaa gtgagagagc ttgttgtatc cttcgatatc agtccctgca cccgtgttgg
     4981 caactaaagc actgcccgtc tcctggttaa ccccatgtgc aataccgccg gtatagacaa
     5041 tcagcgagcc tccggcgcta atattgctgc caattgccgt accatctttc tcaacaacct
     5101 gccggctccc ggaggatata attgttgtgt cagctttccc gccgcttttg atattttgcg
     5161 ttccgctgtt gatattggtg cctgtggcta tgccataatt attaatattc tgtgtgccac
     5221 cattaattat ggtatttgtc gcgtttcctg caacatccat aaccccgcca ttatctattc
     5281 gggtcgcatc agctttagca ttggttaaaa ctgacattgt tcctttatct ttaataatcg
     5341 tcttgtttgc cgaaccatat gcgtttatgt ctaaatgacc accgttttcc agcaacacat
     5401 tgtctgccac gtgattgtgg atggagaatg caccttcact attcgtaccg ctcaccgtcg
     5461 taccgttagt gttagttttt aaaattgcac catcgtgctg ggtaacattt gttgccgtac
     5521 caccactaac atcaagcacg ccaccggaat aaacttcaat aacatccgag gagctggtgt
     5581 tatcaataat ttgcgtacca ccagaataga tctgagtatt ttttgccgtt gacttactat
     5641 tcagagactg agttccgcct tcaatcgtcg tgtccagcgc acggctctca tatactcttt
     5701 gctcaccgcc atttttgatg gtggttgttt ctactgtgct ctgttcaaca tactgtcgac
     5761 caccatttat ggttgtgttc gttgccagac ttccttgtac tacgtcctga gagccagact
     5821 tatttatcgt tgtaccatca gcatgccctt gaacttttac tatctggcta ccaccatcaa
     5881 tgagtgttcc attcgcactc cctccctcta cgcgtgaagc accgccctta attgtcgttc
     5941 cattgctgat acccccttta taaacgtcct gattgccact ctcgattatc gtacctgtgg
     6001 aaataccccc gtcatgaatt gactgtctgc ccccgttaat ggtggtatta ttagactgcc
     6061 ccacataatt gttatgcctt cctacatctt gatatccacc agattcaata agacttccat
     6121 tagatacccc gccataaaca ttctgctggc catggttgat aatatgagtg ttatttgttg
     6181 tacctcgttc atctactcgt tggctgccat ctacagtctc atcgtttacc acaccaataa
     6241 catcaggagt gaaggcagcc atcccgggcg gggcatatat caaggcagat atcaataagg
     6301 aaagtactga gcgacgacaa taatagggac tggtcctgtt cataaatttc atcctctgaa
     6361 aagtgaatac tgagtagcgt ttaagcgacc ttagctttgc tgcaacatca gcccacaggc
     6421 accagaccag gggattcatc ctgaagagac agcgcaagtg tattgtgttc accgctcatc
     6481 aaagacatca tgatgaaatg atgatattcc gcataagaat gaggcatttt ttaaacgcag
     6541 tgcgctgaag tgtggttgga taaaaaagtc aatccattca ggaaatacgg gcgtattctt
     6601 ttctttcgac aatgaggccg ttggcaaaat aaaatgattt acataatcgt ttctgatgaa
     6661 tatcttctgc tcacataaaa atcacacaat aactttgaga tcgcagattg ttttactttt
     6721 acagcattcg tccccccatt gttgggcaaa tatagattgg gccagagcac gaaagttaat
     6781 accacgttcg cacagctcct ccaacagcac gacaagatgc cacatactgc gccccagtcg
     6841 gttcagttta cagactagca gtgctttccc tgccgatgat gtcctgagca gttttttcag
     6901 tccggacctc ctgccacggt tttacgttga agcggtaacc ctgagcacgc agttcttcag
     6961 tcaggcgtgg tgcaccgtaa cgctgttatt gctgggtgag atcaaaaaac tttcaggcag
     7021 ctaaggaaag ttgaaccaga caatagatga aatatttcaa ccaattacag caccaattca
     7081 gtcactgcca gcccaccaaa taaatcaagg ggttacatga aaacgtagcc cctttttctt
     7141 tggtagtgac actaaaatgg atgtagtgtg aagaataatc ccgtttactc aatcaataat
     7201 acatattgtt tcaatctacg ttattatctc tttataaaaa ttgccattta tttatcattg
     7261 aaaactgctt ttagaacttt atacaagagg actattcaca aca
//
LOCUS       DABSYT020000166         7218 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.167, whole genome shotgun sequence.
ACCESSION   DABSYT020000166 DABSYT020000000
VERSION     DABSYT020000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.167"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..898
                     /locus_tag="ID725_RS23125"
                     /old_locus_tag="pgaptmp_004625"
     CDS             <1..898
                     /locus_tag="ID725_RS23125"
                     /old_locus_tag="pgaptmp_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGV7853074.1"
                     /translation="ATVLGIAQANVSKYLADFESKVGLKVFDRTTRQLMLTLFGTALL
                     PYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGIITYLSKHVIDKIKDIGDITLSL
                     KTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYASQRYLEKHPI
                     SRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSALELARN
                     HLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYQPWRVQYVLDEMLR
                     EIRHQLAQSQQLRPEQAAESED"
     gene            complement(895..2031)
                     /locus_tag="ID725_RS23130"
                     /old_locus_tag="pgaptmp_004626"
     CDS             complement(895..2031)
                     /locus_tag="ID725_RS23130"
                     /old_locus_tag="pgaptmp_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGV7853075.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(2037..2360)
                     /locus_tag="ID725_RS23135"
                     /old_locus_tag="pgaptmp_004627"
     CDS             complement(2037..2360)
                     /locus_tag="ID725_RS23135"
                     /old_locus_tag="pgaptmp_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGV7853076.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2543..2686
                     /locus_tag="ID725_RS23140"
                     /old_locus_tag="pgaptmp_004628"
     CDS             2543..2686
                     /locus_tag="ID725_RS23140"
                     /old_locus_tag="pgaptmp_004628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853077.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            complement(2905..4443)
                     /gene="aldB"
                     /locus_tag="ID725_RS23145"
                     /old_locus_tag="pgaptmp_004629"
     CDS             complement(2905..4443)
                     /gene="aldB"
                     /locus_tag="ID725_RS23145"
                     /old_locus_tag="pgaptmp_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGV7853078.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4551..5846)
                     /locus_tag="ID725_RS23150"
                     /old_locus_tag="pgaptmp_004630"
     CDS             complement(4551..5846)
                     /locus_tag="ID725_RS23150"
                     /old_locus_tag="pgaptmp_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGV7853079.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(5976..7127)
                     /gene="yiaY"
                     /locus_tag="ID725_RS23155"
                     /old_locus_tag="pgaptmp_004631"
     CDS             complement(5976..7127)
                     /gene="yiaY"
                     /locus_tag="ID725_RS23155"
                     /old_locus_tag="pgaptmp_004631"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGV7853080.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1812 a         1878 c         1716 g         1812 t
ORIGIN      
        1 cgcgactgta cttggcatcg cacaggccaa cgtcagcaaa tatcttgctg attttgaatc
       61 aaaagtgggt ttaaaagtct ttgaccgaac cacccgacaa ctgatgctca cactttttgg
      121 caccgcgcta ttaccgtaca tcaacgacat gctggacaga aacgagcagc ttaataattt
      181 tattgcagat tataagcatg agaaacgtgg tcgggtcacc atctacgccc caaccggcat
      241 aattacctat ttatccaaac atgtaatcga taaaattaaa gatatcggtg atatcacctt
      301 atcgctaaaa acctgcaatc ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga
      361 tgattgtgat gttttaatca gctatgcccc accaaaagat gaatcgctgg ttgccagttt
      421 tattacccaa tatgccgtaa ccgcttatgc cagccagcgc tatcttgaga aacatcctat
      481 tagccgtcct gacgaactcg aacatcactc ctgtattttg atcgactcga tgatgatcga
      541 cgacgcgaat atctggcgct tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt
      601 gaagggaaat tacgtttgcg ataacacgca atcggcgctg gagctggcac gaaatcacct
      661 ggggattgtg tttgcgccag acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt
      721 gccctgcttt cagcacccgt atgaatggtg gctggatctg gtggctatct tccgtaaacg
      781 ggaataccag ccctggcggg tccagtatgt gctggatgaa atgctgcgtg aaattcgcca
      841 ccagcttgct cagtcgcagc aactgcggcc tgaacaagcg gcggaaagcg aagattaatg
      901 atcgagataa aggtaatgca cccagctggt catgcgtaat aacactttgc gcatcaccga
      961 gacatggctg aaatgatcag agtagaccgc cacctgagcg taaataccgt ctggtaaagc
     1021 gctcaaatca gtgtgctcat tcagttcaat ggtcgcgata acgccatctg aacccggcgc
     1081 tgtgtttaac gtctgtaagg tgccggtcga ctgataagct ccgccgggaa cagctggact
     1141 aatggctgcc agcttaccgc tgaacacttt gcctggcaga gcgttaaata ccacttccgc
     1201 atcatcgccg ggagccaggc gcagcaagga gttctgacgg aactgcgcca cgatttgtcg
     1261 tttctgatcg ggtataaaca ccatcaccgg acgtagcggc agcgacgcgg cataggtacc
     1321 cggacgaatc agcacttggg tcacataacc atcgctcggc gcacgcacta tcgtctgctc
     1381 aaggttatat tttgcttccg cgagctgcgc tttcaggctg gcgatttgag aatgttcacc
     1441 caacaccagg ctatccagct ggctctggat ctgtttttgt tccgccgccg atgactttac
     1501 ggaggcttcc tgcgccagat aattttgccg tgccacatca atatcgcgtt ctgaaaacgg
     1561 gtttactttc gcctggctgc cgcgtgcgta acgctggtac tccttagcga atttatcccg
     1621 cgtggccttt gccttctgag tattcgccgc catctcatct aactctgcgc ccaacgcccg
     1681 ctgtttatgt tctgcggtaa cgatatccgc catcagccga tccacccgcg cctgataacg
     1741 cgtcgggtcc agtcgaaata gcacctcacc ttttttaatc agcgtatttt tcttatccgt
     1801 cacttctatc accacgcctg tcacctgtgg gacaacagga atagaaataa ccgctttttg
     1861 cgctttaaag gtatacggat ggttatagtt cattaacaga attagaccgc tgacaataaa
     1921 tattccaccc agggccgctg tgggaatggt ccatttattt acgggaattt tgaagatctt
     1981 aaacattgcc catgcaaaag ccacataggt caaaataatc agtaaatcca taagtattac
     2041 tcggcagaag aggatttgat gtcggcgagc tgtttctcca gcccggcaat gcgctgttgc
     2101 agttgcataa cggatgaatc atggctttgc attccccatc cccgctccgg gcggtaaagc
     2161 gtggcccaaa tccacaaaaa tggccagata acatgcagcg taaaaagact cacccaacca
     2221 gcaacatgaa tggcgtcggc atgaggatga ttacgttttt tagcaatcag gtagggaata
     2281 tcatgtaaaa taataatccc atagaatatc accagaaata caaaaataag cactcccagt
     2341 gcaaaatagt ccaggaacat atttccctca gagaatataa aaaagaacaa ttaacgcata
     2401 ttatgaaatg ccatgatgca atgcaatttc cttatttata taacgacata ttgtttttta
     2461 tatactttct gaagtgtgct gcgtggcttt ttaacaggtc gaggcttatc atattgaaat
     2521 aatggaacta tcagccaaac taatgaataa cttcagtaaa acatccacaa tgcagattaa
     2581 gcgtattaaa ccttcaataa tctaccgttt attgctgatt gtcctcggaa taccaatggt
     2641 gatttacggt ctggtttgcc cgctcaccat cgaaacgcga gattaaactc ctggcaaaac
     2701 acgccccaga aacgcacttt tgttggcgta gtctgggtta ttgcacacgt aggtttaaaa
     2761 cgtaaattgt aacaacgtgc gctttgttta tgccggatgc ggcgtgtacg ccttatccgg
     2821 cctacggtca ggtccccata ggcatgataa gacgcgtaag cgtcgcatca ggcaattaat
     2881 acccgatgcg accagcttct tacatcagaa cagccccaac ggtttatctg agtagctcac
     2941 tagcaggcac ttggtttgct ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat
     3001 acctgattgt ttgtagccac caaacgccgc atgtgccggg taagcatgat aacagttggt
     3061 ccacacgcgc ccagcctgta tgccgcgccc catcttatag gccagattac cgttgcggct
     3121 ccagacgccc gcgcccaggc catattgcgt atcgttcgcc agctccagcg cttcttccat
     3181 cgttttgaag gtagtcaccg ccagcaccgg gccaaaaatc tcctcctgga acacgcgcat
     3241 attgttctga ccaaacagaa tcgtcggttc gaggtagtag ccgtctttca gttcaccttc
     3301 cagcagcttg cgccgcccgc cggtgagcac atcagcgccc tcttttttac cgatatcaat
     3361 gtagttgagg atggtttcca gttgcccgtg agaaacctgc gcacccattt gcgtcacgct
     3421 gtcgagtggg ttaccgctgc gaatgctttc gacacggcgg atggcgcgtt ccataaagcg
     3481 ttcgtagatt gactcctgca ctaaggcacg actcggacag gtgcaaactt cgccctggtt
     3541 aaaggcaaac agtgcaaagc cttccagcgc tttatcgaaa aaggcatctt cttcatccat
     3601 cacatcagca aagaagatat ttggcgactt accgcccagc tccagcgtca ccggaataat
     3661 gttttgcgtg gcgtattgca taatttgttg acccacttcc gttgagccgg taaacgccac
     3721 tttggcgatg cgtttcgagg tcgccagata ttcgccaatt tccccacctg caccgttgac
     3781 cacgttcacc acgcccggcg gcagtaaatc accgacgatt tccattagca gcagtacaga
     3841 aagcggggta agacgtgcgg gtttcagcac cacacagttg cccgccgcca gcgcgggagc
     3901 cattttccag ctcgccatca gcagcgggaa gttccacggg ataatctgcc ccaccacgcc
     3961 taacggttca tggaaatgat aggccacggt ttcgctatca acttcactga tcccaccttc
     4021 ctgcgcccga atacacgagg cgaaatagcg gaaatggtca atcgccagcg gtacatccgc
     4081 agcactggtt tcgcgaatgg gtttgccgtt atcccaggct tcagctgtcg ctaacagctc
     4141 gaggttttgt tccattcgat cggcaatctt aaacagaatc gccgcgcgat cctgcaccga
     4201 ggtgtgcgcc catttatctt tcactttgtg cgcagcatcc agcgccagat cgatgtctcg
     4261 tttgcccgaa gacgccactt cgcacagcag ctgcccggtc accggcgtca ggttctggta
     4321 atactcacca tcggcagggg ctacccattc gccgccaata aagttgtcat agcggttttt
     4381 taacttgagg gggaaaccat actcgccggg cttaatctgt gctgaagggg gattattggt
     4441 catgatcgtc tccttgcggt gtgaggtata acaagggtag acgtggctgg cgaaatcttc
     4501 gccagtcggt aacagctttt cgacggctat cacgaattta cgggcaaggg ttacagcgga
     4561 taatgcgcga agtacacggc ttgctggtca ttaattttct tctcttgcgc aaacccttct
     4621 ttaacaattg tccgtaaatc agctcgaacc gtattatcag agacgcccag gctcgcgcta
     4681 atttcagcaa ctgtgtactc tttccccgga ttggcgagca tcacattcag gagcgttacc
     4741 tggcgcggat tcaggcgttt aagcacacca gagctaaaca gcacactatc gagttttgcg
     4801 cttcgttgtg taatttcatc gatatgctgc tgccagtttt gcagagcacg tttaatgaca
     4861 cccgcctgat attcaaggaa ataagtcaga tccataccat ctgattcagt gtactggtag
     4921 ctggcggcat atttgaccgg tgcagcatga agcaaacggc tgatggaaat atatttaaag
     4981 acatcgtagc ccgatttcag catataccag taaaacagcg cccgactggt gcgcccgttg
     5041 ccatcccgaa acggatgttc atgggccagc ataaaatgca gaatgcaggc acggaccaac
     5101 ggatgaatat acccttcatg gctgtttaac cactggcaaa ctttttccag ccgttccggt
     5161 aacaatgccg cagcgggcgg ttgatgcact atattgccat catagtcggc gatcacaaca
     5221 tcatctgttt cacgaaattc tcccggacga tatttcgcgt cattgattcc acccatacca
     5281 atggcatgta actggcgaat aagcgcaggt gtcagcggtt ctgcgagtaa gtgcggaatt
     5341 tccgccatca gccgggcatt tcccgcaatc atatgctcgt cttcagtgcg tggcgaccgt
     5401 cctgtttcca gcatttttct cgccaccagc gttgtagtat tcgcaccttc cagctgggag
     5461 ctggtgatcg gctcttccag gcgtaactgg cttaactcgg ctccggcccc gcgtaaccgt
     5521 tcagtctgat ctttcagcgc cagcgcggta gcacgtttgt cgacaagctc gcacgcttct
     5581 gcaatcaccg gggtaatgtt gaatccggcc tgttcacccg cttcattgca ataattaatt
     5641 cgttggatcg ccgcgttgcg cgccagacgg gtaagcgtcc acgccacatc gacgttttct
     5701 ccttttttga tgcggtactg caactcatca aaaggcagat aacgtccttt ttcatcggta
     5761 acccggttaa agcgcaggtg ggcctggagt tcctcacccg ataagtgtga taaaggaatc
     5821 agcgtgggag gtgatttgat agccatttgc ttatctcaaa aaagcgcaat attgagatag
     5881 tgtatagcag gaaagagaaa ctatctcaaa aaaagcaagt tttgagatag ttgaaaaaat
     5941 ggctgacaat ttgccagccg ttgtggaaat gatgattaca tcgctgcgcg ataaatcgcc
     6001 acaatttctt cgtgagttgc ctggatcggg ttagtaaagc cacaggcatc tttcagggca
     6061 ttagccgcca gaaccgcgaa atcttcttct ttcacgttca ggtcgcgtag gcctgccggg
     6121 atatccactt tcttcgccag ttcacggatg gcgttaatgc aggcttcagc accttccgcg
     6181 tcgtttttac ctgtcacgtt cacgcccatt gcagcggcac agtcacgcag acgtgcagcg
     6241 gcgactttgc tgttgaatac ctgaacatgc ggcagcagaa cggcgttaca tacaccgtgt
     6301 ggcaggttgt agaaaccgcc cagctggtgc gccatcgcat gaacataacc cagagaagca
     6361 ttattgaacg ccataccggc gaggaactgg gcataagcca ttgcttcacg cgctttcgca
     6421 ttactgccat cttcaacggc taacggcagg ttttcggcaa tcatggtcac ggctttcagt
     6481 gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat agcgtgcgtt
     6541 aaggcatcca tacccgttgc ggcggtcagt gacttcggca taccaatcat cagagaggag
     6601 tcattgacag aaagcagcgg agtgacatgt ttatcaacaa tcgccatttt gatatgacgc
     6661 gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc cgtggtattg
     6721 atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcgta atcgcgaata
     6781 tcgccgccat tggctgccac cagcgcaata ccttttgcgc agtcgtgtgg agaaccaccg
     6841 cctaaggaga tcacgctatc gcaattattc tctttaagta atttcaaacc tgcggcgacg
     6901 ttttccgtgg tggggttagg ttgggtgcca tcataaataa cgctaaaaat attgcgttct
     6961 tccagtgctt tttgcacatc gcccgccata cctaatttcg ttaacatact gtcagtgaca
     7021 attaaggtac gggtaaatcc ataatctgcc atcatattca ttgcatcagt caatgaatca
     7081 gcgccgatga cattcacaga aggaataaag aaagttgaag atgccataac acactcctga
     7141 aagtgtaaaa aagggtgggc agcttactga ggattttcat tcgaaaacat gatcattatt
     7201 attaattatc aaaattaa
//
LOCUS       DABSYT020000167         7045 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.168, whole genome shotgun sequence.
ACCESSION   DABSYT020000167 DABSYT020000000
VERSION     DABSYT020000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.168"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(580..897)
                     /gene="yehK"
                     /locus_tag="ID725_RS23160"
                     /old_locus_tag="pgaptmp_004632"
     CDS             complement(580..897)
                     /gene="yehK"
                     /locus_tag="ID725_RS23160"
                     /old_locus_tag="pgaptmp_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGV7853081.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            complement(959..4588)
                     /gene="yehI"
                     /locus_tag="ID725_RS23165"
                     /old_locus_tag="pgaptmp_004633"
     CDS             complement(959..4588)
                     /gene="yehI"
                     /locus_tag="ID725_RS23165"
                     /old_locus_tag="pgaptmp_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGV7853082.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            complement(4598..7045)
                     /locus_tag="ID725_RS23170"
                     /old_locus_tag="pgaptmp_004634"
                     /pseudo
     CDS             complement(4598..7045)
                     /locus_tag="ID725_RS23170"
                     /old_locus_tag="pgaptmp_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1714 a         1705 c         1738 g         1888 t
ORIGIN      
        1 atgtgaatat ttatggtggg ctattggcaa aaaaatatct ggaaagtaga tcgcagaaaa
       61 taaaaaaacc tgctggtgag gcaggttctt ttatttacta cagtgacgct aacttgctga
      121 ttgtgcaaca tttcgtacca acgaataaat tataaccata tccacactcc attgcaattc
      181 agtgagtgag atttataatc ctgcccgttg ggaggttgtg ccggatcgga ccaattttaa
      241 gtcctggctg ccggacgggt ggtgccgcag gcggtgccct gatccggaga gcaacgtttc
      301 gtaccaacat acgtaaacca gtatcgacag gcagtgtaag ggtaaggtgc tggtgttgcg
      361 ctccttaata aggagagcgg atggacacgc tgacacaaaa gttaaccgtg ctcattgccg
      421 tactggagtt attagtggct ctgttacggt tgattgattt gttgaagtaa cgggcaaatt
      481 gaaacgcatc taagggggaa taccgtttcc ccctttaaga aagctatact cgtcatactt
      541 caagttgcat gtgctgcgac tttaattatc tagagtatat tattctattt cttggttgaa
      601 tatattctgt agttcacgat taaaaattgc tattacagtt tcaggtgatt cacctcgtaa
      661 tttccgctca tcaatacact catctcttat ataaatcaac gcatcattta ttagcgggtc
      721 agcatcttct cgatcacgtt gatgaaatgt tgaacgtagc cggaatcgct ccagtcgata
      781 gacggaacct gtaaataatt cagagcattt atggatctca agtctgaagg aaaacggatc
      841 ttcaggtaca gggacctcat aatcgtaaat agcaaccagc tctttttgca cgatcatcgc
      901 tttccccgct ggtaagtcgt gttgataacg ggcagttgca tcactgcccg ctgtgttttt
      961 aatggaaaat gacgtcaggt gcgcttaacg cctcactcaa attaaacaca tcaagttcgg
     1021 caaaagtgac ggatttattg tcacggtaca ctcgaagaga acggaagctc attaacgctt
     1081 tgctatcaac attgtagtgg ttaaaaccgg gtgaaaactt aaggaccagc cggtactcac
     1141 ccaggtcata gacaaatgcg tcctcataac ctgactccca gcctttgtat cgcattccca
     1201 taagttgccc aacggtagcg gatttacctt cccagcgagt caggctgtta ctggttgact
     1261 catcaggtgt gagcagcacc gtgcggcgcg ccaactggcg aaaaggcggc ataatttcgt
     1321 aatcggcaaa aagctgtgca aattcactgc gcatttctgc tgtcatttct aacgggtggg
     1381 caatgccaat cagagcgtcc gcaggcaggt caattggctc atcttgcgca ttgcagaacc
     1441 ccccctctgc ggccacacga aaggcgttga gtaaacgacg cggttcattt gccggataaa
     1501 ccccccatat taatcgctgg gtaaccagac gggtaaaggg atgattaaca aacagagaat
     1561 gaaaatctgc cagcgaccag cgtcgggtgg tacgtagggc agcttcaaga cgcgggagca
     1621 ggtttttgct cacctgagta gcatcttttt ttagcccttt tagtcgggcc agtgcctcgg
     1681 gcgctttcag ttgatcgtca tcggcgcgca accggggaac gcttttctgg cgaacgtttt
     1741 gctgatcaaa gataaccggg ttgaggtttt catcgaagcg aacggtaaac tgccggggac
     1801 caaaatcaaa actcaacgcc tgcggatcat caaggcccag ggttgggact aatctgtccg
     1861 ctagctcttc ctggcttagc ccgcgatttt ctgcgactac ctgaagaaat tccgccgcgt
     1921 tatcacgtaa ggggcgggat ttagcccgtt gcgatatatg atgcagctgt atcagcgcca
     1981 tatcattatt catcagggta aggatgttca ggccgctgac agcacgggca gatttgcctt
     2041 gtggccaggc gaggatctgt gttgttagat cccgtgccgt gctttcgtca ccaaagagac
     2101 ttaacgccag aaatgcccag ttgtcttttg cgggggcacc agcttgttgc cacgcagtga
     2161 agagatccca ggcaaaagct gccagcgttt gtggctggca gaacgttttc agttgttcca
     2221 gcccgctata aaaacgtccc ccctgggtaa agcgcagcat ttcgccgata atttccagag
     2281 cgtcatcggt aacaggttga ttattagtga taaggcgcgg cctggaccac atttgcggat
     2341 gccagaaatc aggggctttt ggaatgcgtg ccgggtaaat ttccatggga ccctgtttca
     2401 gtaaatgctc cagggcaggc catacatctg tacgctgcca acgattggct acagtttgta
     2461 gcaattcgcc atgcccatgt tcgtaaagta aacgcagggc aagtaatgca gcttcgcttt
     2521 tatcgctaga tttggtgaag acaagaggga taagtgcagt agccgtatgt tccggccatt
     2581 gtaaaatcca ctggcgagcc agatcacgct gcgccgcgaa acgtcgccag acgcgggcaa
     2641 cgggcagggc cagttctgtt gcgccgaaat ttaaaattaa cggaaatgtt tcttttggac
     2701 gatgtgaaaa agccaacagc agacctggta gcgcatccaa tccgaggata ctgagaaaat
     2761 agtcctctcc cgcgaacaga tgctttttct cattgatacg cagccagaaa gtcaaagcca
     2821 tctcacgcgg caactgtgcc aataaataca ggttccagac actcttttcc ggacgataac
     2881 gtgagtagtt gtcattttcc catatgttaa ttaacgcttc tatgtgaccg ctgcggagcg
     2941 cctgttctgc taattgtgct tccggggagt tccagccatt caggatctct tcaacccgcc
     3001 cctggtagta atgccactga acaatgagtg aattgcgtgc agcatccagt tttccaggga
     3061 gtatataatt caaaaacagc catacatcat ccccaaaacc cagttcctgt aatacaactc
     3121 gcccactttt ttgttccatg cgttgcgcaa gagattccgt gctgaaataa cgggcagact
     3181 cagtggcggc aagcctctct cgttcgcctg gttgccagta aacttgcgga gttaattcga
     3241 gccgtgccaa atcaagacgt ggagctgtca tttttttctt actacgccac ggtggtgaaa
     3301 cgaggatttc aggcagattg ctgttatcgg tgccatcaat aaccggtttt ggcagtgccg
     3361 ataaataact cttcagcact gccctggctt gagtacttaa ccacgacatc acttctgctg
     3421 ccagttgtgg ctgcgatgcc atcagcgttt gtaacagcgt acgccagtat tcttctcgag
     3481 ctttttgctg tgcaggcagc tttttgtctt cgatgattgg ataaccaggg cgggctggtg
     3541 gttcttttaa cgccagcagt tctgccagtg cggcgagcgt cgcatggggg aagtttttac
     3601 tgtatttagc cacacgttgc aggctgggtt tgtttttatc agcaactaac agcaaggtgc
     3661 ggatgacttg cggatggtta atttgtacca gcagactgcc gcaatcttct ttatgagcat
     3721 acattgccag gcgcggaacg gctgccagtc cttgttcgcg caaaagggag gcgcaggccg
     3781 cgtagccgaa gcggttttct tgtgacatgt agctggcttc gcgatcgcta aatacgtcca
     3841 ggccccagta tcgttcgagt ttttttaccg ccgtattatc agtagcaacg acctttaacc
     3901 actcctttga atggagactt gaccgggagc tttcaagact ggcgagttca ttggctattt
     3961 caggtttttc cgggaggatc agcgcaataa aaggacggcg aattttgggt attcctggca
     4021 gtgcggcaat gagtttatcg gcacaacgct gccagcaact ttcctctgcc agcgacagat
     4081 gcttacgcaa acggagatcg aaatcgttat aggtcgcgga gccataaccg tagccatagt
     4141 cctcgtcctg atactgcaaa gtgacgacca gtgagtcact ctcataacgc cgcgcaatgc
     4201 actgacgcga tatcactaca tccgtcgcat attccagccc ctcttgttgc acgatttcat
     4261 caagtaactg ctggctgtca ttttgcgcga tacagatcag agccgccatg gtaagggctg
     4321 ggattgacgg tttatgctcg ccagtcaaat tgattgcctg ctggatggcg gactgccact
     4381 ccagtgagca tttatcataa ttccaggtga tattttcctt ctggcctgct tgttgtagta
     4441 attcatctcc caatgtctgg atgagacttc cagtgataac ggacactggt tcgcccggcc
     4501 agcgacgatg actgagcggc gttttgcctt ttttatattt cagttccagt tgggcgttat
     4561 ccgccagcca cggtaattcc ttgtccatgc tctttcctta ttcaaacagg gcttcaatat
     4621 cgttaattag ctcgctggcg gtgatggcat cgagtacgga gaactgcgct tcctgtgttg
     4681 aattactccc ccagccatag ctttctgcgt tgccttccca tagccacagc ttgcttaaga
     4741 tttgctcggc actcagttcg gctggcgaca tgcctaccgc aaaaccttca tcgatttcca
     4801 tgattaacga ccagcggccc agaggtttga tcatccagcc gatccagcct ccatcctgcg
     4861 gggtgccctt tatccagcct ttattcgcca gccccatgac ccgaccgctc gggcattttc
     4921 tgcctgccca gcgggtcagt tcgctggcgt tgcgctcggc ttctgtcagg gcgtaactgt
     4981 tacggtcaag ctgacggaac ggtggtagca gttcatagtc ggcaaaaagc tgaccaaagg
     5041 cggtagcatc cgtcggtgat atttccagaa cgtgaggaat gccaacagag atatcgcctt
     5101 ccggcagggt gaaaagatca tcgtcagcgg tgctgtagct gttatcttcc gccacgcgga
     5161 agcacgtcag tagctggttt tcggtgctat aaacgcccca aatcagacgg cgggttaagt
     5221 ggcgtaccag tggatgttca accaggaaga gctgaaagtt ttctggcgac cagcggcgac
     5281 gcaggcacat ggcggattcc aaccttgcta cctgctgggc ggcgacggta cgcgcatctt
     5341 ttttcagcag tttgtagcgg ttaacagcat cgttcgcctg tgattcatca tcgcttttgt
     5401 ttggtttggg caggtctttc agacggctgc cagaaacatc acgcacaaac ggttttaagg
     5461 tttcatcaaa gctgacggtg aactggcgtg agccgaaatc cagcagcaac gaaccgttat
     5521 catccagacc gagatccggt gctaaccgat cttcaagctc tgccaccgtg agttcgcggc
     5581 tttcggcaat gccggcaatt ttttcttttg cccgctcctg taatgctttg aatttcagtt
     5641 tctgggcaat accgttaagc tgcatcaggg cgatatcact accgatagcg gcgagaatat
     5701 ccaacccaac ggtggcgcgt ttatgctggg attcaccagg ccaggcgcgt attaatggcg
     5761 tcagtttgcg ggcggtgtca tcgttaccaa gaacgcctaa cgcagtgaac gcccaactct
     5821 ctttcgacgg tgcgcccgca gtcagccagg cggtaaacag atcccaggca aattcagcca
     5881 gtgagtcggc ggtacaggca tctttcacct gcaataatcc cggatacagg gcctcttcct
     5941 gagggaagcg gagcatttca ccgaggtgct ggagtgcgct atctggcagt accttgctgc
     6001 tatttttgag tttcgggcga gtccataatg gaggttgata aaacaacggt aaagggggga
     6061 ttttcgccgg gtatttaccc acaggactgt tgtcgttatt tttattttga gtttcccagg
     6121 gcggcgagac tagtaccatt ggtagaattt ctgagtttgc aaaatcttca ttgcgactat
     6181 tcagcaattg ctgacattgt tgcaataaat ttgccgcttg cgacgataat ccgatgacta
     6241 atttttcgac aaataaagga tttgcattaa gtaatgaaac cagcaatgta tgtttgcgtt
     6301 tatcatcttt ttttaccaat gcttcggcta atgccactat cgttgcagaa ggaaaatttt
     6361 cggcactttt tattcgtaat ggccaacgga tgattaatac atttcagtac gtcaccgcaa
     6421 ttatcttctt cagcataggg agtcaggcga gccagtgccg ctataccttg ctcccgtaat
     6481 accgtagctg accaactggc accataggtg ttaaatacat caatgtattg atagttctcc
     6541 agtatttcga gcgtgaatgg atctgtcgct accagtttca acatttcaag ttgtggaaca
     6601 tctgcgacct ggtgtaaacg atgtacgatc tcatgggcca cttcgggtat ttctggcatt
     6661 aaaatggcaa ttaacggttg gcgatagagt gatatatctg gtaacgcaac gagtaatttt
     6721 gcaatgcaat tttgccatac ttcttcttct gctagtgata aatgtttacg cagacgtaaa
     6781 ctaaatttgt cgttattcct tctataactg tccttgctgc tatctgcata attaataaag
     6841 aaagtcaaat gttcatcttt atagtcaaaa tcgaaatcaa tttgccacca gaagatgaca
     6901 gtttcagttg cgtattcgat accgaaggtg cttacaattt catccagcaa ctcggatgag
     6961 gtttcatcat tccagccctg acaaaaacat atttccagaa aagtaagtac cgccatagta
     7021 cgcgcgggta tctcagtctt ttggg
//
LOCUS       DABSYT020000168         6966 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.169, whole genome shotgun sequence.
ACCESSION   DABSYT020000168 DABSYT020000000
VERSION     DABSYT020000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.169"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..697
                     /locus_tag="ID725_RS23175"
                     /old_locus_tag="pgaptmp_004635"
     CDS             <1..697
                     /locus_tag="ID725_RS23175"
                     /old_locus_tag="pgaptmp_004635"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HGV7853083.1"
                     /translation="RRTKPRQKTLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLR
                     HGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRK
                     SRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKV
                     YFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKT
                     PKEIIERGVALTD"
     gene            complement(705..971)
                     /locus_tag="ID725_RS23180"
                     /old_locus_tag="pgaptmp_004636"
                     /pseudo
     CDS             complement(705..971)
                     /locus_tag="ID725_RS23180"
                     /old_locus_tag="pgaptmp_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            1129..2313
                     /locus_tag="ID725_RS23185"
                     /old_locus_tag="pgaptmp_004637"
     CDS             1129..2313
                     /locus_tag="ID725_RS23185"
                     /old_locus_tag="pgaptmp_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGV7853084.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNLKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIEDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            2313..2825
                     /locus_tag="ID725_RS23190"
                     /old_locus_tag="pgaptmp_004638"
     CDS             2313..2825
                     /locus_tag="ID725_RS23190"
                     /old_locus_tag="pgaptmp_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HGV7853085.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDDESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            2880..3245
                     /locus_tag="ID725_RS23195"
                     /old_locus_tag="pgaptmp_004639"
     CDS             2880..3245
                     /locus_tag="ID725_RS23195"
                     /old_locus_tag="pgaptmp_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853086.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            3254..3409
                     /locus_tag="ID725_RS23200"
                     /old_locus_tag="pgaptmp_004640"
     CDS             3254..3409
                     /locus_tag="ID725_RS23200"
                     /old_locus_tag="pgaptmp_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGV7853087.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3396..6199
                     /locus_tag="ID725_RS23205"
                     /old_locus_tag="pgaptmp_004641"
                     /pseudo
     CDS             3396..6199
                     /locus_tag="ID725_RS23205"
                     /old_locus_tag="pgaptmp_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            6212..6700
                     /locus_tag="ID725_RS23210"
                     /old_locus_tag="pgaptmp_004642"
     CDS             6212..6700
                     /locus_tag="ID725_RS23210"
                     /old_locus_tag="pgaptmp_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853088.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
     gene            6857..>6966
                     /locus_tag="ID725_RS23215"
                     /old_locus_tag="pgaptmp_004643"
     CDS             6857..>6966
                     /locus_tag="ID725_RS23215"
                     /old_locus_tag="pgaptmp_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HGV7853089.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKHEVIRRKRMDIW"
BASE COUNT         1710 a         1674 c         1985 g         1597 t
ORIGIN      
        1 aaggcgaaca aaaccacgtc aaaaaacgct gcgaatatca cctgagacaa tttataaaac
       61 gctgtacttt cgtagccgtg aagcgctaca ccacctgaat atacagcatc tgcgacggtc
      121 gcatagcctt cgccatggca ggcgtcatac ccgcaaaggc gaaagaggta cgattaacat
      181 agtgaacgga acaccaattc acgaacgttc ccgaaatatc gataacagac gctctctagg
      241 gcattgggag ggcgatttag tctcaggtac aaaaaactct catatagcca cacttgtaga
      301 ccgaaaatca cgttatacga tcatcctcag actcaggggc aaagattctg tctcagtaaa
      361 tcaggctctt accgacaaat tcctgagttt accgtcagaa ctcagaaaat cactgacatg
      421 ggacagagga atggaactgg ccagacatct agaatttact gtcagcaccg gcgttaaagt
      481 ttacttctgc gatcctcaga gtccttggca gcggggaaca aatgagaaca caaatgggct
      541 aattcggcag tactttccta aaaagacatg tcttgcccaa tatactcaac atgaactaga
      601 tctggttgct gctcagctaa acaacagacc gagaaagaca ctgaagttca aaacaccgaa
      661 agagataatt gaaaggggtg ttgcattgac agattgaatc tacaagtgcc tttttctgtc
      721 agcggttctc ctttccagcc aatgagaacc gtcaggcgcg cgccccgtgg cggtagctgc
      781 aactgaccat ctgcatcatc cagcgtgatg gtgagctggt ccgcctcaaa tccccggttg
      841 tcggtcagtg acaggctcat caggcgctct gccacgcctg acagcgtttt accctccgcg
      901 agaatatcaa aatccggcac tttcacgtgg tctgtgccct gactgagcaa ttgcatggtg
      961 gtgtcggtca tctgttccct ccctgtgcgg catggtcgca tgtgcgtgcg gagggggtta
     1021 ctgctttttg ttgtcgccgt ggcgggagaa cggcgcaggg gtgagattac gcgcgtggtg
     1081 ggtgatgatt gttgccgaat catttaacgg atacaagggg ctgaagctat gagtgaaact
     1141 cgttttcatg gtgcccgtgt tacggaaagt accgacctgg taacagcgat taatgatgtt
     1201 gattccagtg ttatcggtat cgtggcaacg gcagatgatg cggacgcgga gctgttcccg
     1261 ctgaacaagc ccacactgct gacccgcgtc aatgacgtgc tgggaaaatg cgggactacg
     1321 gggacgcttt atcgtgcgct taaggctatc gcagaccagg tgagcacaaa agtgatcgtc
     1381 gttcgcgtgg ctgaacacaa agaagaaggc ggtaagacgc aggatcaact ggttatcggt
     1441 ggttctgaat ctgacggcag ctatacgggg atgtatgcgc tgcttgttgc agagcaggat
     1501 gaaagcatcg gataccgtcc gcgtattctg gccgcgccgg agctggacac ggaggcggta
     1561 acaaaatccc tgtgcgtgat tgcgggtaaa ctgcgcgcgt ttgtgtatgc cacatgtcat
     1621 ggttgtaaca cgatggctga ggcgattacc taccgccaga aattcaacga acgtgaggtg
     1681 atgctcttat ggcctgactt catcgcctac aacctgaaaa gtggcaaaaa cgaaacgttc
     1741 cccgcgcctg cttatgcgtg cggccttcgt gcgtacattg accatgagca gggctggcac
     1801 aaatcgctgt ccaacgttcc ggttaaaaat gtgcttggga tgtcgaggca tgtgttctgg
     1861 tcgttgcagg ccgaagacag tgatgccaac agcctcaaca acaaagaaat cacgaccatt
     1921 attcgtcgca acgggttccg cttctggggc aaccgcacac cggaaacgaa cgcctacatc
     1981 tttgaggtgt atacccgaac cgcacaggtg ctggctgatt caattgcgga agcgcagttt
     2041 gaaaccatcg acagtccact gacacctgcg aacgtgaagg atgttatcag tgccatcagg
     2101 gcaaaactgg attcactggt tactgccggg aaactgattg gcgcggagtg ctggtatgac
     2161 atcgaggata acagcaccac gaatttacgt caggggcgtg tgcgtattcg ctacaaatat
     2221 acgcccgttc ctccgctgga agacatggag ctttaccagt cgtttactga tgaattcttt
     2281 ggtcccgcat ttgcggtgct gggaggtgcc tgatggctgt accaaaacat cttcgctttt
     2341 ttacgctgtt tgtggatggt gaaaacgaag tgggtaaggt gacgtccgtc accctgccta
     2401 aactgacgcg caaaaccgac agctaccggg gtggtggcat gatgggtgcg gtaagtattg
     2461 acctcggcct ggacgactcc gcgcttgatg cgagctttgt catggggggc gcagttcgtg
     2521 agctgttcct gaagtatggc ggcacgattg acggcacgct actgcgtttt gcgggggaat
     2581 actacaccga tgatgaaagc gacctgtatg aagtcgaaat gcgcggacgt gtgacggaaa
     2641 ttgatatggg ggaagccaaa cagggcgaag ccacatcaca cacttacgcc attaaaaaca
     2701 cctactacaa gctgagtgtt aacgatcgcc cgttgtggga gattgacctg ctgaacttca
     2761 tttaccggaa ggacggcaag gacattgtgc ccgatcgcat ccgttccgcg cttgggcttg
     2821 gctgataagt aatatgcagg cggcgcagtg cgtcgcctct gactgaaagg agtttcctga
     2881 tgaaagagac gaaaaacatc gataccgaaa acacggtagt tactgacact gtgaaagaaa
     2941 ccagtgagcg tggtgtaaaa cttacccaac caattgagcg aggcggcgaa aaaatcacgt
     3001 atgtggagat caccggggct attgagcagg ctggatctct gcgagatttg tcgctgtctg
     3061 atgtgctgaa tctgaaagcg gaatccatgt ttacgctgct gtcacgcgtg acatcaccgc
     3121 gactggatga agtgacgatc aaaaaaatgg catcccgtga ctttattcag ttatgtgtgg
     3181 ttgccgtaaa ttttttgagc ggtgcggact ctggcgggaa gaacgaacag gcgacggaag
     3241 cctgatcacg gttgtgtgct ttgagcacat agaagacttt gtggcagata ttgccgttat
     3301 ttttaactgg tcgcccgccg aaatcttcat gatgacgccc ggcgaagtgg ttagctggcg
     3361 tgagcgggcg gcacttcgca gcgggaatgc agacaatgaa gactcttgat atccgggtcg
     3421 ctttcagcgc cgttgacagg ctgacccgac ctgccgaaaa cgcacgccgc ctgatggggc
     3481 agtttggtga ctccatccag cgaacgcagg gggcgatcaa aaatctcgag cgtcaggcgc
     3541 gatcatttga gcgcgcccgc gacgctgtca gtaaagcgga tgctggcatc gtgaaagcac
     3601 gacgccagct taacgccctt aatcagttac aacgcacggg tacagtgctc agcgaaaaac
     3661 aacaaaagct gatgcagcag ttaagcaccc ggcttgaacg cctgaatgaa tcgcgcacac
     3721 gggaaattca gaaaatgagg gaacttggcg gagagctgaa acgccacggc atttccctga
     3781 caggcagcga taacaccatt cagcaggcca tcagacgcac cgaacaatac aacaaccagc
     3841 ttgaacgcga acggcaggcg cttgcgcgtg taacgcaggc gcgtgagcgg tattcgcacg
     3901 cgcaggaaac cgcgggaaaa ctgaaaacag gtggtgcgct ggcaattggt gcggcagcgg
     3961 cgggcggcta tgctgccggg cgttttttgc agcctgcgat cgggttcggc aaagagatgt
     4021 cccgcgttca ggcactgacg cgaatcgaca aaaacagccc gcagtttaag gcgctgcgtg
     4081 agcaggcgtt aaaacttgct gaaacacagt ttactgcgag tgatgccgcc agtgggcaga
     4141 gctttctggc aatggctggt tttactccgc aggccattca ggccgcattg cccggtgttc
     4201 ttaatatggc gctggcaggt ggcgtcgaac tcggcgagac ggctgatata ggctccaata
     4261 tcctcacaca gttcaacctg acagccgatc aaatggaccg ggtgggcgat acgctgacag
     4321 cagcatttac ccggaccaat actgatttac gcgcgctggg cgaaaccatg aagtataccg
     4381 gtccggttgc cgcaaaactt ggtatcagtc ttgaagaagc ggcggccatg gccgggatgc
     4441 ttgccaataa tggtcttcgc ggaagcgatg ctggcacggc catgcgcgca agtctgtccc
     4501 gccttgcatc accgccaaaa gctgcggctg atgcactgaa agagctgggg gtgtcagttg
     4561 ctgacgccag aggcaaaatg cgcccgatgg aggatgtgct gcttgatctc tataaggcga
     4621 cacaaaaata cggacaggtg gaccaggtct ccttcttcaa ggacatcgcc ggagaagagg
     4681 cgttcgttgg tttgcagacg cttgttgcgg cggctggttc aggagagctg caaaaactga
     4741 ccagagaatt gcagggggca aggggagagg ccgatcgcgt cgcaaaagta atggctgata
     4801 atcttgatgg ggacctgaaa aatctcgaca gcgcatggga aggtcttcgt attcgcatca
     4861 gtgatctggt tgacggtccg ctgcgttctg tcacgcagtg gctcacgcgg gtgcttgaaa
     4921 aaatcacctc gctggcgcag gcccatccgg tactgacgcg ccagctactg atagcaggcg
     4981 gtgcgttgct ggcaatgact gcaacgattg gctcgttgtc gctggttatt ggggtgcttt
     5041 acgggaagct ggccaccctg cgtcttggtt ttgacattct tacccggtca atgaatgtcg
     5101 tcagggtgtt acctgcgcta tggggaatgg tgacgggttc cgtttcttta ctgggaggcg
     5161 ttatcggggc gttgttcagt ccggttggtc ttatcgtggc tgcgcttgcc ggagctgccg
     5221 ttcttatctg gaaatactgg gatcctatca gggctttctt tgccggggtg ttcagcggga
     5281 ttatggaaag gctgaccccg ttgcgcgaca cctttgaacg gtttggccct gtttttgacg
     5341 taatcggaag cggttcagcc aggtgtttaa ctggtttaaa tcgctgctgt caccgatgga
     5401 gtccagcaag gaaacgctgg ataaatgtac cagtgctggc gaggtattcg gtaacgttct
     5461 tggtggtgcg ttacagcttg ttctgacgcc tgcaaaaatg ttgctggata cgctggcgtg
     5521 gatacttgaa aaactcggtg tgcttccgga tgaagcggaa agggcgcgca agaaaatcga
     5581 agacgcacaa cgtgcggcca ttcttcagga caaggttgct ctgtttcagg gagacattgc
     5641 gaaaatcaat ccgccgaagt ctgcgggaaa tagcaatggc accggagatg ataaacccaa
     5701 agacaacaaa cctctcacag acagcaatac cggcacgcta cgcagactca gcaaaattgc
     5761 tgataacaca ggtaagctgg ttgatgagac aaaaaaacgc attggccccg gcgatattgt
     5821 ctttaagaac ctgccccgcg cacttgctgt tcgtggggag tggcaggagc ggaagattgc
     5881 gcaggtcagt aagcctgccc ccgcaattaa tatcacaccc gtggtcccgg ctccgctgcc
     5941 tccggcgctg gtccctgttg ttgcggccag ctcccgcccg gtggtggagg ccatacgatc
     6001 gccagtggca tcagttcctg caacttcccg taaccgggag cctgttgcct ccggatttgg
     6061 cggtgaaatt catgttcatc tgcataacgt tgttacgcag aatccccgcg aactggcgaa
     6121 actggtcggc gaaatggtca gggcagaaat ggaacggcgc gcccgtgcca ggcgtggcag
     6181 tttttacgat aaagattgag gagtcatggc catgatgatg atctacggca tgtttgtttt
     6241 tgagctgcgc acgttgccgc atcagcagtt acagcaaaac aaaagctggc gacatgtgaa
     6301 aaatgaacgc gttaaccgtt cagcaagctg gcagtatatc ggtgcaggtg atgatcgcat
     6361 cgttctttct ggtgttcttt atcctgagat tactggtggc gaagtgtcgc tgtcgttgtt
     6421 gaccacgcag gcatatacag gacgtccttg gcctttgatt gatggcgtcg ggcagattta
     6481 cggcatgtat gtcctgaccg gaacgaatac gacccgctcc gagtttgatc gctacggtaa
     6541 ggcgaaaaag atagaatttt cactgactct tgaacgctgt gatgaggatt tgcgggagcg
     6601 cctgcaatcc tcatcgttca gcgatatgct gtccggcttc aaagataagg tgacatcatc
     6661 ccttaatagc gcggccagct cagttaaggg gctgctctga tttaacgtat gtcgccaatt
     6721 tcctgatgaa ggtgactggc gacttgctgt tgtatgtcct tctcagaaaa ttgtttttga
     6781 ataacaaata acaggatttt ataatctctt aaccttataa catgtgtggt ctgaaataat
     6841 aattaaggag attatcgtgc tatcttactt aatggcaatt cactttgttt tatttggaaa
     6901 ctctactaat ttaaaaaact tctggaaaca tgaagtaatt cggcggaaac gtatggatat
     6961 ctggag
//
LOCUS       DABSYT020000169         6770 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.170, whole genome shotgun sequence.
ACCESSION   DABSYT020000169 DABSYT020000000
VERSION     DABSYT020000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.170"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..425
                     /locus_tag="ID725_RS23220"
                     /old_locus_tag="pgaptmp_004644"
     CDS             <1..425
                     /locus_tag="ID725_RS23220"
                     /old_locus_tag="pgaptmp_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGV7853090.1"
                     /translation="SKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGE
                     RYKVKKIIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAA
                     YSLENPGIIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            429..1799
                     /gene="cpsG"
                     /locus_tag="ID725_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /gene_synonym="manB"
     CDS             429..1799
                     /gene="cpsG"
                     /locus_tag="ID725_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGV7853091.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKAL
                     LKLLSE"
     gene            2087..3018
                     /locus_tag="ID725_RS23230"
                     /old_locus_tag="pgaptmp_004646"
                     /pseudo
     CDS             2087..3018
                     /locus_tag="ID725_RS23230"
                     /old_locus_tag="pgaptmp_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            3062..3727
                     /gene="perB"
                     /locus_tag="ID725_RS23235"
                     /old_locus_tag="pgaptmp_004647"
     CDS             3062..3727
                     /gene="perB"
                     /locus_tag="ID725_RS23235"
                     /old_locus_tag="pgaptmp_004647"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HGV7853092.1"
                     /translation="MNLYGIFGAGSYGRETIPILNQQIKQECGSDYALVFVDDVLAGK
                     KVNGFEVLSTNCFLKAPYLKKYFNVAIANDKIRQRVSESILLHGVEPITIKHPNSVVY
                     DHTMIGSGAIISPFVTISTNTHIGRFFHANIYSYVAHDCQIGDYVTFAPGAKCNGYVV
                     IEDNAYIGSGAVIKQGVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKRSP
                     TSI"
     gene            3928..5334
                     /gene="gndA"
                     /locus_tag="ID725_RS23240"
                     /old_locus_tag="pgaptmp_004648"
     CDS             3928..5334
                     /gene="gndA"
                     /locus_tag="ID725_RS23240"
                     /old_locus_tag="pgaptmp_004648"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGV7853093.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            5583..6749
                     /gene="ugd"
                     /locus_tag="ID725_RS23245"
                     /old_locus_tag="pgaptmp_004649"
     CDS             5583..6749
                     /gene="ugd"
                     /locus_tag="ID725_RS23245"
                     /old_locus_tag="pgaptmp_004649"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGV7853094.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
BASE COUNT         1949 a         1411 c         1570 g         1840 t
ORIGIN      
        1 tgtctaaaaa gagtgatgta cagcatgtaa aaaaaatagt cgaaatgctt aaattgcagc
       61 aacgtacaga gtatattagt catcgtgaag ttttccgacc atggggaaaa tttgattcga
      121 ttgaccaagg tgagcgatac aaagtcaaga aaattattgt gaaacctggt gaggggcttt
      181 ctttaaggat gcatcaccat cgttctgaac attggatcgt gctttctggt acagcaaaag
      241 taacccttgg cgataaaact aaactagtca ccgcaaatga atcgatatac attccccttg
      301 gcgcagcgta tagtcttgag aatccgggca taatccctct taatcttatt gaagtcagtt
      361 caggggatta tttgggagag gatgatatta taagacagaa agaacgttac aaacatgaag
      421 attaacatat gaaatcttta acctgcttta aagcctatga tattcgcggg aaattaggcg
      481 aagaactgaa tgaagatatt gcctggcgca ttgggcgtgc ctatggcgaa tttctcaaac
      541 cgaaaaccat tgttttaggc ggtgatgtcc gcctcaccag cgaagcgtta aaactggcgc
      601 ttgcgaaagg tttacaggat gcgggcgtcg atgtgctgga tatcggtatg tccggcaccg
      661 aagagatcta tttcgccacg ttccatctcg gagtggatgg cggcatcgaa gttaccgcca
      721 gccataaccc gatggattac aacggcatga agctggtgcg cgaaggggct cgcccgatca
      781 gcggtgatac cggactgcgc gatgtccagc gtctggcaga agccaatgac ttccctcctg
      841 tcgatgaaac caaacgtggt cgctatcagc aaatcaatct gcgtgacgct tacgttgatc
      901 acctgttcgg ttatatcaac gtcaaaaacc tcacgccgct caagctggtg atcaactccg
      961 ggaacggcgc agcgggtccg gtggtggacg ccattgaagc ccgatttaaa gccctcggcg
     1021 caccggtgga attaatcaaa gtacacaaca cgccggacgg caatttcccc aacggtattc
     1081 ctaacccgct gctgccggaa tgccgcgacg acacccgtaa tgcggtcatc aaacacggcg
     1141 cggatatggg cattgccttt gatggcgatt ttgaccgctg tttcctgttt gacgaaaaag
     1201 ggcagtttat cgagggctac tacattgtcg gcctgctggc agaagcgttc ctcgaaaaaa
     1261 atcccggcgc gaagatcatc cacgatccac gtctctcctg gaacaccgtt gatgtggtga
     1321 ctgccgcagg cggcaccccg gtaatgtcga aaaccggaca cgcctttatt aaagaacgta
     1381 tgcgcaagga agacgccatc tacggtggcg aaatgagcgc tcaccattac ttccgtgatt
     1441 tcgcttactg cgacagcggc atgatcccgt ggctgctggt cgccgaactg gtgtgcctga
     1501 aaggaaaaac gctgggcgaa atggtgcgcg accggatggc ggcgtttccg gcaagcggtg
     1561 agatcaacag caaactggcg caacccgttg aggcaattaa tcgcgtggaa cagcatttta
     1621 gccgcgaggc gctggcggtg gatcgcaccg atggcatcag catgaccttt gccgactggc
     1681 gctttaacct gcgctcctcc aacaccgaac cggtggtgcg gttgaatgtg gaatcacgcg
     1741 gtgatgtaaa gctaatggaa aagaaaacta aagctcttct taaattgcta agtgagtgat
     1801 tatttacatt aatcattaag cgtatttaag attatattaa agtaatgtta ttgcggtata
     1861 tgatgaatat gtgggctttt ttatgtataa cgactatacc gcaactttat ctaggaaaag
     1921 attaatagaa ataaagtttt gtactgacca atttgcattt cacgtcacga ttgagacgtt
     1981 cctttgctta agacattttt tcatcgctta tgtaataaca aatgtgcctt atataaaaag
     2041 gagaacaaaa tggaacttaa aataattgag acaatagatt tttattatcc ctgtttacga
     2101 tattatagcc aaagttgtat cctgcatcag tcctgcaata tttcacgagt gctttgttaa
     2161 ctgaatacat gtctgccatt ttccagatga taacgacgtc atcgcaattg atggtaaaac
     2221 acttcggcac acttatgaca agagtcgtcg cagaggagtg gttcatgtca ttagtgcgtt
     2281 tcagcaatgc acagtctggt cctcggatag atcaagacgg atgagaaacc taatgcgttc
     2341 acagttattc atgaactttc taaaatgatg ggtattaaag gaaaaataat cataactgat
     2401 gcgatggctt gccagaaaga tattgcagag aagatataaa aacagagatg tgattattta
     2461 ttcgctgtaa aaggaaataa gagtcggctt aatagagtct ttgaggagat atttacgctg
     2521 aaagaattaa ataatccaaa acatgacagt tacgcaatta gtgaaaagag gcacggcaga
     2581 gacgatgtcc gtcttcatat tgtttgagat gctcctgatg agcttattga tttcacgttt
     2641 gaatggaaag ggctgcagaa tttatgaatg gcagtccact ttctctcaat aatagcagag
     2701 caaaagaaag aatccgaaat gacgatcaaa tattatatta gatctgctgc tttaaccgca
     2761 gagaagttcg ccacagtaaa tcgaaatcac tggcgcatgg agaataagtt gcacagtagc
     2821 ctgatgtggt aatgaatgaa atcgactata atataagaag gcgagttgca ttcgaatgat
     2881 tttctagaat gcggcacatc gctattaata tctgacaatg ataatgtatt caaggcagga
     2941 ttatcatgta agatgcgaaa agcagtcatg gacagaaact tcctagcgtc aggcattgca
     3001 gcgtgcgggc tttcataatc ttgcattggt tttgataaga tatttctttg gagatgggaa
     3061 aatgaatttg tatggtattt ttggtgctgg aagttatggt agagaaacaa tacccattct
     3121 aaatcaacaa ataaagcaag aatgtggttc tgactatgct ctggtttttg tggatgatgt
     3181 tttggcagga aagaaagtta atggttttga agtgctttca accaactgct ttctaaaagc
     3241 cccttattta aaaaagtatt ttaatgttgc tattgctaat gataagatac gacagagagt
     3301 gtctgagtca atattattac acggggttga accaataact ataaaacatc caaatagcgt
     3361 tgtttatgat catactatga taggtagtgg cgctattatt tctccctttg ttacaatatc
     3421 tactaatact catataggga ggttttttca tgcaaacata tactcatacg ttgcacatga
     3481 ttgtcaaata ggagactatg ttacatttgc tcctggggct aaatgtaatg gatatgttgt
     3541 tattgaagac aatgcatata taggctcggg tgcagtaatt aagcagggtg ttcctaatcg
     3601 cccacttatt attggcgcgg gagccattat aggtatgggg gctgttgtca ctaaaagtgt
     3661 tcctgccggt ataactgtgt gcggaaatcc agcaagagaa atgaaaagat cgccaacatc
     3721 tatttaatgg gaatgcgaaa acacgttcca aatgggacta atgtttaaaa tatatataat
     3781 ttcgctaatt tactaaatta tggcttcttt ttaagctatc ctttacttag ttattactga
     3841 tacagcatga aatttataat actctgatac atttttatac gttattcaag ccgcatatct
     3901 agcggtaacc cctgacagga gtaaacaatg tcaaagcaac agatcggcgt agtcggtatg
     3961 gcagtgatgg ggcgcaacct tgcgctcaac atcgaaagcc gtggttatac cgtctctatt
     4021 ttcaaccgtt cccgtgaaaa gacggaagaa gtgattgccg aaaatccagg caagaaactg
     4081 gttccttact atacggtgaa agaatttgtt gaatctctgg aaacgcctcg tcgcatcttg
     4141 ttaatggtga aagcaggtgc aggcacggat gctgctattg attcccttaa gccatacctc
     4201 gataaaggtg acatcatcat tgatggtggt aataccttct tccaggacac cattcgtcgt
     4261 aaccgtgagc tttctgcaga aggctttaac ttcatcggta ccggtgtttc cggtggtgag
     4321 gagggcgcac taaaaggtcc ttccattatg cctggtgggc agaaagaagc ctatgaacta
     4381 gttgcgccga tcctgaccaa aatcgccgca gtggctgaag acggtgagcc atgcgttacc
     4441 tatattggtg ccgatggcgc aggtcactat gtgaagatgg ttcacaacgg tattgaatac
     4501 ggcgatatgc agctgattgc tgaagcctat tctctgctta aaggtggtct gaacctcacc
     4561 aacgaagaac tggcgcagat ctttaccgag tggaataacg gtgaactgag cagctacctg
     4621 atcgacatta ccaaagacat cttcactaaa aaagatgaag acggtaacta cctggttgat
     4681 gtgatcctgg atgaagcggc aaacaaaggt acgggcaaat ggaccagcca gagcgcactg
     4741 gatctcggcg aaccgctgtc gctgattacc gagtctgtgt ttgcacgata catctcttct
     4801 ctgaaagatc agcgcgttgc tgcgtctaaa gttctctctg gcccacaagc gcagccagct
     4861 ggcgacaagg ctgagttcat cgaaaaagtt cgccgtgcac tgtatctggg caaaatcgtt
     4921 tcttacgctc aggggttctc tcaactgcgt gcggcgtctg aagagtacaa ctgggatctg
     4981 aactacggcg aaatcgcgaa gattttccgt gctggctgca tcatccgtgc gcagttcctg
     5041 cagaaaatca ccgatgctta tgccgaaaat ccgcagatcg ctaacctgct gctggctcct
     5101 tacttcaagc aaattgccga tgactaccag caggcgctgc gcgatgtcgt cgcttatgcg
     5161 gtacagaacg gtatcccggt tccgaccttc gccgctgcgg ttgcctatta tgacagctac
     5221 cgcgccgctg ttctgcctgc gaacctgatc caggcacagc gtgactattt cggtgcgcat
     5281 acttataagc gcattgataa agaaggtgtg ttccataccg aatggctgga ttaatctgat
     5341 ttaatcaaca ataaaattga ggcccggcgt atattgtacc gggctttttt ttgccaatta
     5401 tcttatagac taaatatcac tgcttaatat taacttaata aatatcagct attcttataa
     5461 agaaaatctg aattgttttt cgctgcgttg accatcgaac aacgtagcgt taaaactttt
     5521 agctcttatc aggatgttaa aaacatcatg attcacagtt aagttaattc tgagagcatg
     5581 aaatgaaaat caccatttcc ggtactggct atgtaggctt gtcaaacggg cttctaatcg
     5641 ctcaaaatca tgaggttgtg gcattagata ttttaccgtc acgcgttgct atgctgaatg
     5701 atcggatatc tcctattgtt gataaggaaa ttcagcagtt tttgcaatca gataaaatac
     5761 actttaatgc cacattagat aaaaatgaag cctaccggga tgctgattat gtcatcatcg
     5821 ccactccaac cgactatgat cctaaaacta attatttcaa tacatccagt gtagaatcag
     5881 taattaaaga cgtagttgag ataaatcctt atgcggttat ggtcatcaaa tcaacggttc
     5941 ccgttggttt taccgaagcg atgcataaga aatatcgtac tgaaaatatt attttctctc
     6001 cggaatttct ccgcgaaggt aaagctcttt acgataacct tcatccgtca cgtattgtaa
     6061 tcggtgagcg ttcggaacgc gcagaacgtt ttgctgcgtt attacaggaa ggcgcgatta
     6121 agcaaaatat cccaaccctg tttaccgact ccactgaagc agaagcgatt aaactttttg
     6181 caaacaccta cctcgcgatg cgcgtggcgt actttaacga actggatagc tacgcagaaa
     6241 gtttaggtct gaattcccgt caaataatcg aaggcgtttg tctcgaccca cgtattggca
     6301 accattacaa caatccgtcg tttggttatg gtggttattg tttgccgaaa gataccaagc
     6361 agttactggc gaactaccag tctgtgccga ataacctgat ctcggcaatt gtcgatgcta
     6421 accgcacgcg taaagatttt attgccgatg ccattttgtc acgcaagccg caagtggtag
     6481 gtatttatcg tctgattatg aagagcggtt cagataactt ccgtgcgtct tctattcagg
     6541 ggattatgaa gcgtatcaag gcgaaaggtg ttgaagtaat catctacgag ccagtgatga
     6601 aagaagactc attcttcaac tctcgcctgg aacgtgatct cgccaccttc aaacaacaag
     6661 ccgacgtcat tatttccaac cgtatggcag aagagcttag ggatgtggca gataaggtct
     6721 acacccgcga tctctttggc agcgactaac atcttgttat cagggctatt
//
LOCUS       DABSYT020000170         6745 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.171, whole genome shotgun sequence.
ACCESSION   DABSYT020000170 DABSYT020000000
VERSION     DABSYT020000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.171"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..820)
                     /locus_tag="ID725_RS23250"
                     /old_locus_tag="pgaptmp_004650"
     CDS             complement(<1..820)
                     /locus_tag="ID725_RS23250"
                     /old_locus_tag="pgaptmp_004650"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 carboxypeptidase N/E family protein"
                     /protein_id="HGV7853095.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEENAANADTSAGDASESAR
                     QAAESAAAAKQSEEASSSSASAAAQKASESSQSAAEAELSRKTAESAAGNASRDATTA
                     AEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEQGPAGPQGPKGDKGE"
     gene            complement(885..1484)
                     /locus_tag="ID725_RS23255"
                     /old_locus_tag="pgaptmp_004651"
     CDS             complement(885..1484)
                     /locus_tag="ID725_RS23255"
                     /old_locus_tag="pgaptmp_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGV7853096.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTSAPG
                     SDNLNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(1552..2274)
                     /locus_tag="ID725_RS23260"
                     /old_locus_tag="pgaptmp_004652"
                     /pseudo
     CDS             complement(1552..2274)
                     /locus_tag="ID725_RS23260"
                     /old_locus_tag="pgaptmp_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            2262..2678
                     /locus_tag="ID725_RS23265"
                     /old_locus_tag="pgaptmp_004653"
                     /pseudo
     CDS             2262..2678
                     /locus_tag="ID725_RS23265"
                     /old_locus_tag="pgaptmp_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            2806..3342
                     /gene="yfdR"
                     /locus_tag="ID725_RS23270"
                     /old_locus_tag="pgaptmp_004654"
     CDS             2806..3342
                     /gene="yfdR"
                     /locus_tag="ID725_RS23270"
                     /old_locus_tag="pgaptmp_004654"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGV7853097.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            3333..3683
                     /locus_tag="ID725_RS23275"
                     /old_locus_tag="pgaptmp_004655"
     CDS             3333..3683
                     /locus_tag="ID725_RS23275"
                     /old_locus_tag="pgaptmp_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HGV7853098.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            3680..4153
                     /locus_tag="ID725_RS23280"
                     /old_locus_tag="pgaptmp_004656"
                     /pseudo
     CDS             3680..4153
                     /locus_tag="ID725_RS23280"
                     /old_locus_tag="pgaptmp_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            4300..4497
                     /locus_tag="ID725_RS23285"
                     /old_locus_tag="pgaptmp_004657"
     CDS             4300..4497
                     /locus_tag="ID725_RS23285"
                     /old_locus_tag="pgaptmp_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853099.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEVLMILCRSAMLSQN"
     gene            4455..4805
                     /locus_tag="ID725_RS23290"
                     /old_locus_tag="pgaptmp_004658"
                     /pseudo
     CDS             4455..4805
                     /locus_tag="ID725_RS23290"
                     /old_locus_tag="pgaptmp_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            4842..5123
                     /locus_tag="ID725_RS23295"
                     /old_locus_tag="pgaptmp_004659"
     CDS             4842..5123
                     /locus_tag="ID725_RS23295"
                     /old_locus_tag="pgaptmp_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HGV7853100.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(5171..5344)
                     /locus_tag="ID725_RS23300"
                     /old_locus_tag="pgaptmp_004660"
     CDS             complement(5171..5344)
                     /locus_tag="ID725_RS23300"
                     /old_locus_tag="pgaptmp_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853101.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(5541..6074)
                     /locus_tag="ID725_RS23305"
                     /old_locus_tag="pgaptmp_004661"
     CDS             complement(5541..6074)
                     /locus_tag="ID725_RS23305"
                     /old_locus_tag="pgaptmp_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853102.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            complement(6221..6325)
                     /locus_tag="ID725_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /pseudo
     CDS             complement(6221..6325)
                     /locus_tag="ID725_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT         1796 a         1676 c         1530 g         1743 t
ORIGIN      
        1 gctctccctt atcacccttc ggcccctgag gacccgcggg cccctgttcc ccctttggcc
       61 cgggaggtcc caccacggtg gggattctgt ttacggcttc ttccgccgct atcctgcttt
      121 gttccgctga ctgtgcgctt tctgctgact cccgggcttt ttctgctgcg gtcgttgcat
      181 ccctggatgc attaccggct gcactttctg ccgtctttct tgacaattca gcttctgctg
      241 cactttgtga tgactcactg gctttttgag cggccgcaga ggccgaggac gaggacgcct
      301 cctctgactg ctttgcagcg gctgcacttt ctgccgcctg ccgggctgac tccgatgcat
      361 cccctgctga agtgtcagca tttgcagcgt tctcttctgc ctgactggct gatatgccgg
      421 cattcctcgc ggacgtctcc gcctctccgg cattcttctt cgcctcctca gcgtgacgcg
      481 ccgcttcttc caccatcagt tcaaaacgac gcagtgcctc cggccggacg tcatcctccg
      541 acatggcacc gagaaaatca ttcagcgtac cgggttgaga atcttcatac acggtgatgg
      601 tcccggcatg tgacggcggg aatccttcca ccaacagaat gacgctgtac tgaccgtact
      661 caacgtccat gctgtaacga ccggcttcat ccggattttc agaggccacc gtgttcacca
      721 ccaccgtgct gctggtccgt ctggctttca gttgaatggt gcagttctct accggttttc
      781 ctgtgccgtc tttcagtaca cctgaaatct ttactgccat attcacccca caaaaaagcc
      841 cgcctgaacc ggcgggctgt cataacactg tgttacctgg ctaatcagaa tttatagccg
      901 acacccacga tgaagccgtc agtgcgccag tcgccactgc cggagccttc ataagcaata
      961 tcaatggcca cggattcggt cgggttaaac tgcacgccag ctccccacgc cagagacgtg
     1021 ttgctgtggc gaccgtcatc acttccggtc agcacatcat gcgttttccc cttgttgtca
     1081 gttacgcgga gataatcccc ggagaaagtc gacacacggc tgtaagccac acccgccatc
     1141 gcatacgcgc tgaaccattc attcacgcgc acagagggcc ccgccatcac gctgaaccag
     1201 cggttacgca cggaatcttc atgccagcgg gtatcgctgt aacgggtaag ctggcgattc
     1261 ctgtctcctg catagctgaa tgacgtcacc atccccagtg tgtccgtaaa ctcataacgg
     1321 tatttcacgt taatcccgtt aagattatcg ctaccgggag cgctcgtccg ggcatgaaga
     1381 taccccgcgc tcagcgtggc ctgctgctca gacgcccatg caggcgcgcc ggatacggcc
     1441 agacagatgg ctgcggacaa aatggcggca taaagtttac gcataattac ctctcgcttt
     1501 tctgcaataa aaaaggcgcc atttctggcg cccgtatatg ggttataaaa ttcagctgat
     1561 actgatgcct gcggtggctt tcttcatcac cacaaccagc aaatcgctga tacttgctgt
     1621 gggataccag tcatttacca gccatgctga caccgaaaac tccagcgtca tgtgaccgtg
     1681 accggcaggc atatcaataa cgccactgta aatcagcgta ttatccagcg cggtacggtt
     1741 ataaatttca gcaccgtttt tccgcactat cagacggcat gaggagtaaa tatcagtatg
     1801 ctctctctca tgcttagcgc cactgaatgc caccgccgga ataacaatct gccggtcaaa
     1861 cggctgatcg tcataaaccc tgacggtaat ggtccctgat ggccaccgtt ccggtgcacg
     1921 ggagtcccgg gggaaagctt tgcccactgt tttaacgaga tcgccttcaa tctggttcgc
     1981 ggacagtttt cccagaaccc gacagttctc gttaatcgtg acgttgttga gcgtcccgga
     2041 gttcgcattc acgttaccac tgatatccgc atttttagca gtcagctttc catccggtgt
     2101 cagggaaaat gccggtggat ttccaccact ggtaatggtg ggggccgtca ggcgcttcag
     2161 gaacacgtcg ttcatgaata tctggttgcc ctgcgccaca aacatcggcg tttcattccc
     2221 gtttgccggg tcaataaacg cgatacgatt ggcggcaacc agaatggatt gaagactggg
     2281 ccgactacct tgtgggcttt gatgctaatg gtgacaccat tcaggcaaca aaagcggctg
     2341 cggcggtccg taaaatcacg attgaagcaa accagaccgc tgattttgaa gataatgact
     2401 tcagcggcaa acgctccctg atggagtctg tcgaagcgaa gaccaaagac attatgccag
     2461 tggcatttga atttaaatgc attccgtttg aaggtctgaa agaacgtccg tttaaattac
     2521 gcctcagcat tatcactggc gatcgtccgg tactggttct gcgcattatt cagctggagg
     2581 cggtgcagga agaaatggct aacgaatttc gtgatctgct tgttgagaaa ttcaaggaca
     2641 gcaaagtaga aacctttatt ggtactttca ccgcctgatt tcattactgc aaatgcccct
     2701 gagggggcat ttatggaaac gtaatttact caataatcgc cggatggtga gggattcttt
     2761 ttaccagaat tcagcgcggt gcagcgcata tacgtggaga acaaaatgtc atttattaaa
     2821 actttttccg ggaagcattt ttattatgac aggataaata aagacgacat cgatattaac
     2881 gatatcgcgg tttccctttc aaatatctgt cgctttgccg gtcatctttc gcacttctac
     2941 agcgtcgccc aacatgcggt tctttgcagc cagctggtac cgcaggaatt tgcttttgaa
     3001 gcattaatgc atgatgcaac agaagcgtat tgccaggaca tccccgctcc actgaaacgc
     3061 cttcttcctg actataaacg gatggaagaa aaaattgacg ccgtaatccg tgagaaatac
     3121 gggttacccc cagttatgag tacgcccgtg aaatatgccg atcttatcat gctggcaacc
     3181 gaacgccgcg atctcgggct tgatgatggc tctttctggc ctgtactgga aggcatcccg
     3241 gcaacagaga tgttcaacgt gattccactg gcacctagcc atgcctacgg gatgtttatg
     3301 gaacgtttta acgagttatc ggagttacac aaatgcgcat gaatgttttc gaaatggaag
     3361 ggtttcttcg cgggaaatgt gtaccgcgag atctgaaagt gaatgaaaca aatgctgagt
     3421 atctggtgcg taaattcgat gaagtacgtg ctgaggctcg caacgagggt attaactata
     3481 ccgcaagccg tcttgctgct gctttcaatc acggatttat caataagcct ttggctgaag
     3541 ttttcgacgt tacacgcatg attctgtcag caaaagaaga gttagctaat gaatcgcatc
     3601 cgattgatgg cctgtccggt gaatatgcag agaaatccct tgaagaatgg gcggaacggc
     3661 ttcgcaaagg aggcagccag tgactggaca tgcagcaatc ctcgacatgt gctgtggcag
     3721 tcgcatgttc tggttcgata agaatgacga ccgggcgata tttagcgata tcagaaagga
     3781 agagcacaca ttgtgtgatg gacgacgcct gattatcagt cctgatctga tagctgattt
     3841 tcgtgcacta ccatttgcag acgcatcttt ttcgatggtt atattcgacc ctccgcatct
     3901 tgagcgtgtt ggtgataacg cctggatggg aaagaaatat ggacggctga ataaagatac
     3961 ctagcgtgat gatttgcggc agggatttaa agaagccttt cgtgtgttgc gtccatccgg
     4021 cgttctgatt tttaaatgga atgaaacgca aatacctgtt cgccagatat tggtactgac
     4081 cgacagaaaa cctgttatcg gtcaacgaac aggaaaaaac gataaaaccc actggattat
     4141 ttttatgaaa taggcatcca gtgagtaggt tcgtaaggtt acagatacgt atatctgaat
     4201 aattaaattc agttctgtaa ataaaattta atccttaacc ggagggattc ctgcaccctc
     4261 agaacatcag gagaccgccc gaaagggcgg taatgaaaaa tggctgaatt aaccaaatgg
     4321 ctacaaaaca cgattaccgg aattgaaacg gtagtagacg ataaatcgtt tgtatgtgat
     4381 gaaatagtat tcaaaatcga tgtggttaaa aacgtactta ccgcatttaa agtcgcgctg
     4441 gtatcgctgg aagtattgat gatactttgt cgatcagcga tgctctctca gaactaaacc
     4501 atttcattaa ccggcacgca gacaatacga aatatttaaa agtctggggt aacggggcca
     4561 ccttcgacaa cgtaatttta cgtggagctt acgagcgagc aggacaaatc tgcccgtggg
     4621 cgtactggaa tgaccacgat gtacgcacga tcgttacgct tgggcgttcc atcggattcg
     4681 accccaaaat ggacatgcct ttcgatggcg aacggcacaa cgccctggct gatgcccgtc
     4741 atcaggcaaa atatgtttcc gctatctggc agaaattaat tcctgccacc agcacagaat
     4801 tatgattttc ccgggtgcag ccggttttga tggagaaaat tatgaacacc ttgtttttac
     4861 tgatggctga attcaatacc ccaaacattg aactctcagc agttagccaa aagtactttg
     4921 gtatgagtcc agccacggca gaagcaaaag caaacgcttg taagttgccc gttccaacat
     4981 atcgcatcgg cacatcacaa aaagcaaaac gttgcatcaa tattcaagat cttgcggaat
     5041 acatagacaa aaggcgagaa gaaggacgta tcgagtggga acaggtcaga acagtcaaac
     5101 agaaggacaa agaacatcac taaagaaaaa acccgcctga aggcgggttt tcaaaaagca
     5161 ccagctatga tcatgctgct ttgcgacgac gaagcttacc ctgctgctct ttaccagaga
     5221 cagtagcgtg agtgaacgca ttaggagcag ccttcatcag aacttcaaca gcagcaccca
     5281 tacctgcgaa tgctttcatt gtgtcgaact taacctgtgg cttggttgct ttttgatctt
     5341 tcatagaaaa ctcccgagac agtaaaggcg tctctaaccc tttctttaaa gctagcttgt
     5401 ttcgctaact tatgccaatc gatcatgtcg attggtgaca tcgtttctta gtagtttaag
     5461 cacaaaacga ctgccataga tgtaccttta aggtaatctg gacgggtatc ctacaatttg
     5521 tagacccttc tcgtctatac ctactgagca aatttaagaa agatatcctg cagctcatca
     5581 atgactgccg acatcacata accgcactgt tccatgcgga aaccaaaaga ctcgtaatac
     5641 tgcaccagtt ctggtactgg ctctacaatg tggacaactt tacattcaac agctttacaa
     5701 aatataaaag cactcataag agtgagtaaa accatgcgcc ctttcaatgg gtgagattca
     5761 tcttctctag aaaacctttc gatcatatgg atacgaaaga tgttttcttc aaccccataa
     5821 acacaaattg ctgctcctga tggtattccc tgaacccgac cttgctgaac aagttttatg
     5881 cagaactcat acttttctct ggagttgcca taggtactta acgcatagtc ccattcaagc
     5941 tcaccatagc caccacacag aatcttgtaa tcatcatcac tgagcggacc aacagcaaga
     6001 ggtaagccga catgatcaat aatcaactgg atattgttac gtactgattg acctatctcg
     6061 tccagggtaa gcatcatggc ctctcaagcg gaacactaaa agtcgcatta tatctcattc
     6121 ttaagccgcg tatggattac accttgaaat gaaaacgccg ggttcccaat aggctcccac
     6181 aaagtatata actatttgtt tttcaaaaac ggtacatcct atcgagcatt ggtgcaacgc
     6241 taaaccgacc actccagtga acgtcagttt tttcaggcat tgcgctggtt tggttgattt
     6301 tttgcatttc agaattaccg tgcattttca aatgtagaga ttattttatc gatatatcat
     6361 gtggttatgt tattcagcat cactgttcag gaggctcaat aacggggtac tataccataa
     6421 caacaggaag cgcctgtctc attgcaaaag aaaattgaga tcctctcaag gcatgaagct
     6481 ctcacgaagt gaatatctaa tcttattaga tgttagcgtt gtgaaaagca atgataaaca
     6541 atacagattc gacgataaat aaaaatcaca cattaaactc cggtgatatc tctccctgct
     6601 aatagcactg atagagaaaa aaagaaccca ataaacattg ggttctttta tgtaatacct
     6661 ttcataccat cgaagaactt cacatattat ttctccgatt taaccccgaa taaatcataa
     6721 attaatttag aagtgcctgt atata
//
LOCUS       DABSYT020000171         6607 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.172, whole genome shotgun sequence.
ACCESSION   DABSYT020000171 DABSYT020000000
VERSION     DABSYT020000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.172"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(470..1606)
                     /locus_tag="ID725_RS23315"
                     /old_locus_tag="pgaptmp_004663"
     CDS             complement(470..1606)
                     /locus_tag="ID725_RS23315"
                     /old_locus_tag="pgaptmp_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGV7853103.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1609..1971)
                     /locus_tag="ID725_RS23320"
                     /old_locus_tag="pgaptmp_004664"
     CDS             complement(1609..1971)
                     /locus_tag="ID725_RS23320"
                     /old_locus_tag="pgaptmp_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGV7853104.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2508..4421
                     /gene="mtlA"
                     /locus_tag="ID725_RS23325"
                     /old_locus_tag="pgaptmp_004665"
     CDS             2508..4421
                     /gene="mtlA"
                     /locus_tag="ID725_RS23325"
                     /old_locus_tag="pgaptmp_004665"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGV7853105.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4516..5664
                     /gene="mtlD"
                     /locus_tag="ID725_RS23330"
                     /old_locus_tag="pgaptmp_004666"
     CDS             4516..5664
                     /gene="mtlD"
                     /locus_tag="ID725_RS23330"
                     /old_locus_tag="pgaptmp_004666"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGV7853106.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5664..6251
                     /gene="mtlR"
                     /locus_tag="ID725_RS23335"
                     /old_locus_tag="pgaptmp_004667"
     CDS             5664..6251
                     /gene="mtlR"
                     /locus_tag="ID725_RS23335"
                     /old_locus_tag="pgaptmp_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGV7853107.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6263..6472)
                     /gene="yibT"
                     /locus_tag="ID725_RS23340"
                     /old_locus_tag="pgaptmp_004668"
     CDS             complement(6263..6472)
                     /gene="yibT"
                     /locus_tag="ID725_RS23340"
                     /old_locus_tag="pgaptmp_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGV7853108.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         1677 a         1651 c         1665 g         1614 t
ORIGIN      
        1 tcgatgaatg ataacttaat tttatttaaa aatatattaa taactcactc tccaggagtt
       61 ttttaactta gttttataat gaatacgata attaaaagtg aagaagaaaa tagttgtata
      121 catatattcc atgaccctac attgtgatct atagattaaa tattttttaa atgtgactgt
      181 catccttcag ataataaagc catcatcgca atagatggaa aagcactcct gctctttttt
      241 gattagagcc gtcgcaggag gaacaactca tatcatcatt gcgatctcga cgatgtatag
      301 tctcatcctg cggcagaaca agacggataa aaaatccaat gagagcacac tcatccagaa
      361 cttattaaca taatgaatat taaagaaaaa tcatcgcagc taatgaagta atatgcaaaa
      421 ataattagtc cttacaagga gcaaagttcc ataatctccc ccctccccct caatgatcca
      481 aataaagata atgcatccag ctggtcattc ttagcagcac tttccgcatc accgaaacat
      541 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
      601 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggcacgaccg
      661 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
      721 tagtcagttt gccgtgaaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
      781 cgccgggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
      841 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
      901 ggatcagtac ctgagtaacg tagccattgc tcggcgcgcg aatgacagtc tgctcaaggt
      961 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttaa
     1021 ccatactgtc gagctggctc tggatctgcg cctgctccgc caccgagcct ttcaccagcg
     1081 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     1141 ccgccgcctg gctgcctttc aagtaacgtt gataattttt aaacagacgg tcgcgctccg
     1201 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     1261 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg
     1321 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     1381 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     1441 taaaagtgta agggtggttg tagttcatca ataaaatcaa accactcacc agaaacacgc
     1501 ctcccagcgc cgccgtcgcc agcgtccact gatttaccgg aatgcggaag attttaaaga
     1561 ccgcccacgc cagcgccacg taagttaaaa caatcaatag atccataatt agatctccgg
     1621 gaacgtggaa tagtcagtct ttttctcggc ggcgagttga tgctccagcc gggaaattcg
     1681 gtcagaaagt gaggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg
     1741 cataccccag ccacgctccg gttgatagag cgtcgcccag atccacagaa acggccagat
     1801 aacatgcaga gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catgggggtg
     1861 attgcgcttt ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat
     1921 caccagaaag acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc
     1981 ggattcacgt ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat
     2041 tttgacaata atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt
     2101 gatgagttgc ttagttacat aacgattgta tgacgaaggc ataacatgct gtagatcaca
     2161 tcaggtgaac gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat
     2221 caaaacaaaa atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct
     2281 tgttgctttt ttacacaagc gttttgtgat gaacgtcacg tcaattacct ctctaccccc
     2341 tatatttatg tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt
     2401 gccagatttc gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc
     2461 tcgggcttcc agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata
     2521 ttaagatcaa agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg
     2581 gcgcgtttat cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga
     2641 acgagacgct ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg
     2701 gttataccgg tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca
     2761 ccatgggcgt tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc
     2821 cgctgggcgg ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg
     2881 gttttgagat gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc
     2941 tggcattcct cggcattggc ccgattgttg aaaccctgtc caaaatgctg gctgcgggcg
     3001 ttaacttcat ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga
     3061 aaatcctgtt cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc
     3121 agtcccatga actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta
     3181 tgggcgtgct gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg
     3241 gtgcggcaat catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga
     3301 tgaatccgcg tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga
     3361 ctatcctggg cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg
     3421 cgatgacacc aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg
     3481 tctccttcgt tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata
     3541 ttgaagcagc aactcgtcgt atgcaggaca tgaaagctga gtctaaaggt gcatctccgc
     3601 tgtctgcggg cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg
     3661 acgccggtat gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg
     3721 caggtctgtc acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg
     3781 acctcgtcat cactcaccgt gacctgaccg aacgcgctat gcgccaggtt cctcaggcac
     3841 agcatatttc gctaaccaac ttcctcgaca gcggcctgta caccagcctg actgaacgtc
     3901 tggttgctgc ccagcgacat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca
     3961 gctttgacga ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc
     4021 gcaaagcagc aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg
     4081 gttacgttga gccggagtac gttcaggcga tgctggatcg tgaaaaactg accccgactt
     4141 atctgggtga gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga
     4201 aaacgggcgt ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg
     4261 acattgctcg tctggtgatt ggtattgctg cccgtaacaa cgagcacatt caggttatca
     4321 ccagcctgac caacgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca
     4381 gcgtggatga agtgctggaa ctgctggcag gtcgtaagta atccaatcct accctctcct
     4441 tacggagagg gtttgggtga gggaaaagcc tcaccccagc cctctcgggt aaaaacattg
     4501 atgaaggtta atactatgaa agcattacat tttggcgcag gtaatatcgg tcgtggcttt
     4561 atcggtaaac tgctggcaga cgcgggtatc caactgacgt ttgccgatgt caatcaggtg
     4621 gtacttgatg ccctgaatgc ccgtcatagc tatcaggttc atgtggtcgg tgaaaccgag
     4681 caggtagata ccgtttccgg cgtcgatgct gtcagcagca ttggtgatga tgtcgttgat
     4741 ctgattgctc aggtcgattt agtcactacc gccgttggcc cggttgtgct ggaacgtatt
     4801 gctccggcta tcgccaaagg gctggtgaaa cgtaaagaac aaggtaatga atccccgctg
     4861 aacatcatcg cctgtgaaaa catggtacgc ggtaccacgc agctgaaagg tcatgtgatg
     4921 aacgccctac cagaagacgc caaagcgtgg gtagaagaac acgttggctt tgtcgattcc
     4981 gccgttgacc gcatcgtacc gccttcggct tcggcaacta acgatccgct ggaagtgacg
     5041 gtagaaactt tcagcgaatg gattgtcgat aaaacgcagt tcaaaggcgc actgccgaac
     5101 atcccaggca tggagttaac cgacaacctg atggcatttg tcgaacgtaa actcttcacc
     5161 ctgaacacgg gtcatgctat aaccgcgtac ctcggaaaac tggccggtca tcagaccatt
     5221 cgtgacgcta ttctcgacga gaaaatccgc gcggtggtaa aaggtgcgat ggaagaaagt
     5281 ggtgcggtac tgatcaagcg ctacggcttt gacgcagaca aacatgcggc gtacatccag
     5341 aaaatcctcg gtcgttttga gaacccgtat ctgaaagatg atgtagagcg cgtaggccgt
     5401 cagccgctgc gtaaactgag tgctggcgac cgtctgatca agccattgct cggtacgctg
     5461 gaatacagcc ttccgcacaa gaatctgatt caggggattg ctggtgcaat gcacttccgc
     5521 agtgaagacg atccgcaggc tcaggaactg gcagcactga tcgctgacaa aggtccgcag
     5581 gcggcgctgg cacagatttc cgatcttgat gccaacagcg aggttgtatc cgaggcggta
     5641 accgcttata aagcaatgca ataatggtgg accaggcgca ggacaccctg cgcccgaata
     5701 acagattgtc agatatgcag gcaacaatgg aacaaaccca ggcctttgaa aaccgtgtgc
     5761 ttgagcgtct gaatgctggc aaaaccgtgc gaagctttct gatcaccgcc gtcgagcttt
     5821 tgaccgaggc ggtaaatctt ctggtgcttc aggtattccg caaagacgat tacgcggtga
     5881 agtatgctgt agaaccgtta ctcgacggcg acggtccgct gggcgatctt tctgtgcgtt
     5941 taaaactcat ttacgggttg ggcgtcatta accgccagga atacgaagat gcggagctgc
     6001 tgatggcatt gcgtgaagaa ctaaatcacg acggcaacga gtacgccttt accgacgacg
     6061 agatccttgg accctttggg gaactgcact gcgtggcggc gttaccaccg ccgccacagt
     6121 ttgaaccagc agactccagt ttgtatgcaa tgcaaattca gcgctatcaa caggctgtgc
     6181 gatcaacaat ggtcctttca ctgactgagc tgatttccag aatcagctta aaaaaagcct
     6241 ttcaaaagta agcaacgtct gcttactgcc cctctacctg cttcggccga taaagccgac
     6301 gataatactc cagacgttgt agatataacg gcacgctttc atcgggtatt ccggacggaa
     6361 tcgcgttacg gggaggaagt tttttcagat actcccggaa cgcctggctt gatgccatga
     6421 aatctacggc tttatcgata agaagcggaa cgttttcacc taatttgccc atgatgatat
     6481 ctccgtatta ccccgcgccg ggaatgcgcg gccgccaatt ttagtttagg ttcccggtaa
     6541 agatgacatt aaggaaaacg tgctgaatcc tcaataatca tccgctgctg tttattatct
     6601 tttttct
//
LOCUS       DABSYT020000172         6549 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.173, whole genome shotgun sequence.
ACCESSION   DABSYT020000172 DABSYT020000000
VERSION     DABSYT020000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.173"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            91..654
                     /gene="fimA"
                     /locus_tag="ID725_RS23345"
                     /old_locus_tag="pgaptmp_004669"
     CDS             91..654
                     /gene="fimA"
                     /locus_tag="ID725_RS23345"
                     /old_locus_tag="pgaptmp_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGV7853109.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            1015..1725
                     /locus_tag="ID725_RS23350"
                     /old_locus_tag="pgaptmp_004670"
     CDS             1015..1725
                     /locus_tag="ID725_RS23350"
                     /old_locus_tag="pgaptmp_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGV7853110.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1767..4418
                     /locus_tag="ID725_RS23355"
                     /old_locus_tag="pgaptmp_004671"
     CDS             1767..4418
                     /locus_tag="ID725_RS23355"
                     /old_locus_tag="pgaptmp_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGV7853111.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGATFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSTLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            4432..4962
                     /locus_tag="ID725_RS23360"
                     /old_locus_tag="pgaptmp_004672"
     CDS             4432..4962
                     /locus_tag="ID725_RS23360"
                     /old_locus_tag="pgaptmp_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7853112.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4975..5478
                     /locus_tag="ID725_RS23365"
                     /old_locus_tag="pgaptmp_004673"
     CDS             4975..5478
                     /locus_tag="ID725_RS23365"
                     /old_locus_tag="pgaptmp_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7853113.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            5538..6452
                     /locus_tag="ID725_RS23370"
                     /old_locus_tag="pgaptmp_004674"
     CDS             5538..6452
                     /locus_tag="ID725_RS23370"
                     /old_locus_tag="pgaptmp_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7853114.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1865 a         1414 c         1458 g         1812 t
ORIGIN      
        1 aagaatgatt cttgttttat aagcaataac agaatgactt aattgttaac tctctttatt
       61 cagctactta aatataaatt ttggagaatt atgaaactca aacatgttgg tatgattgtc
      121 gtttctgtgt tggcgatgtc gtctgctgcg gtaagcgcag ccgagggtga tgaatcagta
      181 acgaccactg ttaatggcgg tgttattcat tttaaaggtg aagtggtaaa tgccgcttgt
      241 gcgattgatt ccgaatcaat gaaccaaacg gttgagctgg gtcaggttcg ttcttctcgc
      301 ctggctaaag cgggtgacct cagctccgcc gttggcttca atatcaagct gaatgattgt
      361 gataccaatg tttccagtaa tgcagctgtt gcattcctgg gtactactgt caccagtaat
      421 gacgatacgt tagcgctgca gagttcagcg gcaggctctg cccaaaatgt cggtattcaa
      481 attttggacc gtacgggtga ggtattaata cttgatgggg ccacttttag tgctaaaacc
      541 gacttgattg atggcacgaa tatactacca ttccaggctc gttatattgc tctcgggcag
      601 tccgtagctg gtactgcaaa cgcagatgcg accttcaaag ttcaatatct ataattcaat
      661 attaaggtgg ctatacggca tctgaaatta aaacagggtg tgtgtggcat ggctacgcac
      721 acccgagcaa ttcaccaatg tgaatctcat tattaatgat gagtggtttg tatttggagc
      781 gctatgaaaa atcacataat atctaaacgt tcaataaaca acagtagtta aacttactgt
      841 tattaaaaat atttactttt tagcaaggtt ttcctattaa aagagaacca acctttatta
      901 caaacgcatt aagtctggaa acccggtgac tcgccaatgt attagcaaca tttaccgatt
      961 gtctgatgta acggttttcc cgcttatcct cttaacacat ggagaatgca tcagatgcaa
     1021 actacgcgta ccccctactc tatttccttt atggcgacag tgttactttt attgctcttt
     1081 gcttgccata gcaccgttgc caacgcagct gtcgcactgg gtgcgacgcg ggtaatttat
     1141 ccggcaaacc agaaacaggt tttattgcct gtcacgaata atgatcctgc cagtgtctat
     1201 ctcattcagt catggataga aaatgctggt gaccaaaaag acacccagtt tgtcattacc
     1261 ccacctcttt tttccatgca aggtaaaaaa gagaatacgc tgcggataat caacgccact
     1321 aatcatcagt tacccggcga ccgtgaaagc ctgttctggg ttaatgttaa agctattcct
     1381 gctatggaaa aagaccagaa gaatgagaat acattacagc tggcaattat tagccgcata
     1441 aaaatgtttt atcggcccac gaacctggca atggcacctg aagaagcgcc tgcaatgctt
     1501 cggtttcgtc gcagcggtag caatctcact cttattaatc caacgcccta cttcattacc
     1561 gtaacaaata tgaaagcagg aaatagcaat ctcccaaaca ccatggtgcc gccaaaaggt
     1621 gaagtgtcgg tggatatccc gcatgctgcc accggggata tcagttttca gacgatcaat
     1681 gattacggcg cattaactcc gcgtattaag gcaaccatgc agtaagccag tgacctaatt
     1741 aataataaac tcaatcggaa ttaataatga cagcctttcg tgccgcattt aaggcatatc
     1801 gtatgcatca ggtgttacta ctgcctcgct ttgcacggct taccattgcc cttagcttag
     1861 caactgcggt atttcccgtt gacgcagagt attattttaa tccacggttt ttaagcaatg
     1921 atcttgctga atctgtcgat ttatccgcct ttactaaggg acgcgaggca ccacctggta
     1981 catatcgggt cgacatttat ttgaacgacg aatttatgac tagtcgggat attactttta
     2041 ttacagatga taataacgca gatctcatcc catgcctgag tacagatctt cttgtcagtc
     2101 ttggcataaa aaaatctgcg ttattagaca ataaggaaca ttcagcagaa aaacatgtgc
     2161 cggacaacag cgcatgcaca ccacttcagg atcgtttggt cgacgcatca actgagtttg
     2221 atgttggtca gcaacatctg tcactctccg ttccgcaaat ttatgttggc agaatggctc
     2281 gcggctatgt ttctcctgat ctgtgggaag aaggaataaa tgctgggctt ctaaactata
     2341 gttttaatgg taattctatt aataatcgta gtaaccataa tgcaggaaaa tccaactatg
     2401 catatttgaa tttacagagt ggcatcaaca ttggtagttg gcgactacgc gataactcaa
     2461 cgtggagtta taacagtggg agtagcaatt catctgacag caataaatgg cagcatatca
     2521 atacgtcggc tgaacgtgac attattccct tacgctcacg gttaacggta ggtgatagtt
     2581 ataccgatgg cgatattttt gatagtgtga actttcgtgg cctcaaaata aattcaacag
     2641 aagcgatgtt gcccgatagc caacatggtt tcgctccggt gattcatggt attgcccgtg
     2701 gcaccgcaca agtgagtgta aaacagaacg gatacgatgt ttatcagact actgttccac
     2761 ccggcccttt tactattgat gatatcaact ctgcggccaa tggtggtgat ctgcaagtaa
     2821 ccataaaaga ggcagacggc agtattcaga cattatatgt tccttattcg tctgttccgg
     2881 ttctccaacg tgctggatat acgcgttatg cgcttgccat gggggaatat cgtagtggaa
     2941 ataacctgca aagctccccc aggttcatac aaggtagctt gatgcatgga ctggaaggaa
     3001 actggacacc ttatggcgga atgcaaattg cagaagatta tcaggccttc aaccttggta
     3061 ttggtaaaga tttaggactt tttggtgcct tttctttcga tatcacgcag gccaatacga
     3121 cacttgcaga tggcacccgt cacagcgggc aatcggttaa atccgtctac agcaaatcct
     3181 tctaccagac aggaaccaat atccaggtcg caggatatcg ctattctacg caaggttttt
     3241 ataacttatc cgacagtgcc tacagtcgaa tgagtggtta caccgtcaag cctcctaccg
     3301 gagacagcaa tgagcagaca caatttattg attattttaa tctgttctac agtaagcgtg
     3361 gtcaggaaca aataagcatc tctcagcagc ttggaaatta cggtgcgaca tttttcagtg
     3421 ccagtcgcca aagttactgg aacacgtcac gcagcgacca gcaaatatca tttggattaa
     3481 atgtgccgtt tggtgatatt acgacttcgc tgaattacag ctattccaat aatatatggc
     3541 aaaacgatcg ggatcattta ctcgctttta cgcttaatgt tcccttcagt cattggatgc
     3601 gtacagacag tcagtcggca tttcgtaatt caaacgccag ttacagtatg tcaaacgatt
     3661 tgaaaggcgg catgaccaat ctatcggggg tttatggcac tctgctgccg gataataacc
     3721 tgaattatag cgttcaggtc ggtaacaccc acggaggtaa tacatcgtct ggcaccagtg
     3781 gttacagtac tcttaattat cgtggagctt acggcaatac taatgtcggt tacagtcgga
     3841 gtggtgacag cagccagatt tattacggaa tgagtggtgg gattattgct catgctgatg
     3901 gcatcacctt tggacagccg ctgggcgaca caatggttct ggttaaggct cctggcgctg
     3961 ataatgtcaa aatagagaac cagaccggaa ttcataccga ctggcgtggc tatgccatat
     4021 taccatttgc gacagaatat agagaaaatc gtgtcgctct taacgcgaat tcccttgcag
     4081 ataatgttga actggatgaa accgtggtca ctgtcatccc aactcacggt gctattgcca
     4141 gagcaacatt taatgcacaa atcggcggga aagtattaat gacgttgaag tacggtaata
     4201 aaagcgttcc attcggtgca attgtcactc acggagagaa taaaaatggc agcattgtcg
     4261 cggaaaacgg tcaggtttat ctgactggac ttccacagtc agggaaatta caggtttcat
     4321 ggggcaatga taaaaactca aactgtattg tcgattacaa gcttcctgaa gtctctcctg
     4381 gaaccttgct gaaccagcag acagcaatct gtcgctaagg aacaatagat tatgaaatac
     4441 aataacatta ttttcctcgg tttatgtctg gggttaacca cctattctgc tttatccgca
     4501 gatagcgtta ttaaaattag cgggcgcgtc ctcgattatg gctgcacagt ctcatcggat
     4561 tcgcttaatt ttaccgtaga tctccaaaaa aacagtgcca gacaatttcc aacgaccggt
     4621 agcacaagtc cagccgtccc ttttcagatt acgttaagtg aatgcagcaa agggacaacg
     4681 ggggttcggg ttgcatttaa cggtattgag gacgcagaaa ataatactct gttgaaactg
     4741 gatgagggaa gcaatacggc ctccggttta ggtatagaaa tactggacgg aaatatgcgt
     4801 ccggtgaaac tgaatgacct tcatgccggg atgcagtgga tcccactggt accagaacag
     4861 aacaatattt tgccttactc cgctcgtctg aagtcaactc agaagtccgt caatccggga
     4921 ctggtgaggg cttcggcaac ctttaccctt gaatttcaat aatcagagtc agatatgaaa
     4981 agacttcaca agaggttcct cttagctacg ttttgcgcgt tattaacagc aactctccag
     5041 gccgccgatg tcactatcac tgttaatggt cgggtagtcg ctaaaccctg cactattcaa
     5101 accaaagaag ctaacgttaa tctcggggat ctttatacgc gcaatctgca acaacctggt
     5161 tctgcatctg gctggcacaa tattactttg tcattaaccg attgtccggc tgaaacaagt
     5221 gcagtgacgg caatcgtgac aggttcaact gacaatacgg gttattacaa aaatgaaggt
     5281 actgccgaaa atattcagat agagcttagg gatgaccagg atgcgacgtt aaaaaatggc
     5341 gatagcaaaa cggttattgt tgatgagatc actcgtaatg cacagtttcc acttaaggca
     5401 agagctatca cggtgaatgg aaacgcaagc cagggaacga tcgaggcgct aatcaatgtg
     5461 atctacacct ggcaataacg tcgattcgat tttcttagcc ttctttttaa atcaattttt
     5521 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
     5581 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
     5641 ttggagctgg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
     5701 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
     5761 acactgatca tataaacctg gtacaaggct cagctttcgc aggctctcta cagtcatata
     5821 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
     5881 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
     5941 tcggggcggc cggtggagtt gtcataaaag ccggggaagt tattgcccgt attcatatgt
     6001 ataaaattgc cacgttagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
     6061 ataacagtgt tgttatgcca acaggcggct gcactgttga tagtcgtaat gtcaccgtga
     6121 atctcccgga ctttcctgga agcgcagaaa tcccccttgg cgtatattgc tcaagcgaac
     6181 aaaagctctc attttatctt tcgggtacta ccactgacag tgctagacaa gtgtttgcta
     6241 acacagcacc agatgcaaca aaagcatcgg gagtgggtgt ctctctgatg cgtaatggaa
     6301 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     6361 tgggtttaag cgcaacctat ggacaaacag ggaacaaagt ttcagccgga accgtccagt
     6421 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     6481 ccattcacga aaattgcgcg gataattatt ttgtgaaggc tattatccta cacttgtaag
     6541 aaaatccgc
//
LOCUS       DABSYT020000173         6489 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.174, whole genome shotgun sequence.
ACCESSION   DABSYT020000173 DABSYT020000000
VERSION     DABSYT020000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.174"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(87..269)
                     /locus_tag="ID725_RS23375"
                     /old_locus_tag="pgaptmp_004675"
     CDS             complement(87..269)
                     /locus_tag="ID725_RS23375"
                     /old_locus_tag="pgaptmp_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853115.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
     gene            599..1471
                     /locus_tag="ID725_RS23380"
                     /old_locus_tag="pgaptmp_004676"
     CDS             599..1471
                     /locus_tag="ID725_RS23380"
                     /old_locus_tag="pgaptmp_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGV7853116.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVWGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1843..4692
                     /gene="cah"
                     /locus_tag="ID725_RS23385"
                     /old_locus_tag="pgaptmp_004677"
     CDS             1843..4692
                     /gene="cah"
                     /locus_tag="ID725_RS23385"
                     /old_locus_tag="pgaptmp_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HGV7853117.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            4803..>6489
                     /locus_tag="ID725_RS23390"
                     /old_locus_tag="pgaptmp_004678"
     CDS             4803..>6489
                     /locus_tag="ID725_RS23390"
                     /old_locus_tag="pgaptmp_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HGV7853118.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         1636 a         1632 c         1805 g         1416 t
ORIGIN      
        1 cccagattaa accaagtatt gagcgcctga ttatgtttat tcattaatat ctgtcattga
       61 ttgtcttatt atcagctatc cccgtattaa tcagagtttt catttatttt taatgcataa
      121 tcagatgccg cctcatcatt atcctcagta ttatcatagc cgaaaacttc tcccggacac
      181 gggcgttcac gataatagtc atcgtcatag ttttcatcgt cagataatga tatgtcgtat
      241 tctgaatatg attcaccaca attatacatg aaatcatctc ctttgggggg gggcaataat
      301 tagtgagttt attcgaaaaa atgatgagcg ccagaccaat ccacccaact tttgattata
      361 atcatgaaga tacttgtctc agtacaaatt gaatcaaact caatgcagca tgattcacct
      421 ttatattctc tcagggaaac caaataccac tactcaacta ttaagaacaa aattcgggat
      481 gtatgattat cagaagatac ctgcatccta aataatttca gacagatatt atcgttgtga
      541 atcccccgcc acctttccgg tgcgcggggt tttgtctttt ttcaccggga atacatgtat
      601 gaatccgtct gatgccattg aggcaattga aaaaccgctc tcctccctgc cttactcgct
      661 ttcccgtcac atcctggaac atctgcgcaa actcaccagt cacgaacccg tgattggcat
      721 tatgggtaaa agcggggccg gtaaatcctc actctgtaat gcactgtttc agggggaggt
      781 caccccggtc agtgatgttc acgccggcac ccgggaagtg cggcgcttcc gtctgagtgg
      841 ccatggtcac agcatggtta tcactgacct gcccggggtg ggcgagagcc gggacaggga
      901 tgcagagtat gaagccctgt accgtgacat tctgcctgaa ctggacttgg tactgtggct
      961 gattaaagcc gatgaccgtg ccctgtctgt ggatgagtat ttctggcgac acatactgca
     1021 ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacag agccctgcca
     1081 tgaatgggat atggcaggta ttcagccttc tcccgcacag gaacagaaca ttcgcgaaaa
     1141 aacagatgcg gtattccggc tgttccggcc cgtacatccg gttgttgccg tatcggcccg
     1201 caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc ccgaccatgc
     1261 cgccagtccc ctgatgaccc gcctgcagga cgagctgtgc acggaatctg tctgggggca
     1321 ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggtgg agagcgtctg
     1381 tgttgcctct gttgcacgca cagttctgcg tgccgtccgt gacacggtgg tctctgttgc
     1441 ccgcgcggta tggaactgga ttttcttctg aacagtctgc caggggaaat gtctgattac
     1501 attgcgttaa ccaactgaaa acatgataca aacacctcat gcccctcctg tttctcttgc
     1561 gtgactgctc tactgttaat agaataaaac gatcgataaa acaggtatcg atcgtttata
     1621 tcgatcgttt atggtggtta atggcctgcg aaaaggcagc tgaatctctt catcatgcag
     1681 aacggaattg cacacaacag actgatactt ctctgtctga tgaaggggga gagcgtttac
     1741 gcccgggacg tatcgctgtg cccgataact ctgttttgta cctgccggta tccacttttg
     1801 tgggtaccgg cttttttatt caccctctgt aaggaaaagc tgatgaaacg acatctgaac
     1861 accagctaca ggctggtatg gaatcacatt acgggcaccc tggtggtggc ctccgaactg
     1921 gcccgctcac ggggaaaacg cgccggtgtg gcggttgcgc tgtctcttgc tgctgtcaca
     1981 tcagtcccgg cactggctgc tgacaaggtt gtacaggcgg gagaaaccgt gaacgatgga
     2041 acactgacaa atcatgacaa ccagattgtc ttcggtacgg ccaacggaat gaccatcagt
     2101 accgggctgg aactggggcc ggacagtgaa gaaaacaccg gtgggcaatg gatacagaat
     2161 ggcgggatag ccggaaacac cactgtcacc acaaatggtc gtcaggtcgt gctggagggg
     2221 ggaacagcca gtgatacggt tattcgtgac ggcgggggac agagcctgaa cggactggcg
     2281 gtgaacacca cactgaataa cagaggcgag cagtgggtgc atgagggcgg ggttgccacc
     2341 ggtacaatta tcaaccgcga cggttaccag agcgttaaaa gtggcgggct ggcaacagga
     2401 accatcatca acaccggcgc agaaggcggc cctgattctg acaactcgta tacgggtcag
     2461 aaggtccagg gaacagcaga atccaccacc atcaacaaaa atggacggca gattatctta
     2521 ttttccgggc tagcccgtga cactctcatt tacgcaggtg gtgaccagtc ggtacacgga
     2581 agggccctga ataccacact gaatggcggt taccaatatg tgcacaggga cggacttgcg
     2641 ctgaacacgg taattaacga ggggggctgg caggttgtta aggcaggtgg cgctgccggt
     2701 aacaccacca taaatcagaa cggtgaactg agggtacatg ccggcgggga agccactgca
     2761 gtcacccaga acacgggcgg tgcactggtt accagtactg ctgcaactgt catcggcaca
     2821 aaccgtctgg ggaatttcac ggtggaaaac ggtaaggctg acggtgttgt tctggaatcc
     2881 ggcggtcgtc tggatgtact ggagagccat tcagcacaga ataccctagt ggatgacggc
     2941 ggtaccctgg cagtgtctgc cggcggtaag gcgacaagtg tcaccataac atccggtggt
     3001 gccctgattg cagacagtgg tgccactgtt gaggggacca atgccagcgg taagttcagt
     3061 attgatggca catccggtca ggccagcggc ctgctgctgg aaaatggcgg cagctttacg
     3121 gttaatgccg ggggacaggc tggcaacacc actgtcggac atcgtggaac actgacgctg
     3181 gctgccgggg gaagtctgag tggcagaaca cagctcagta aaggcgccag tatggtactg
     3241 aatggtgatg tggtcagtac cggcgatatt gttaacgcag gggagattcg ctttgataat
     3301 cagacgacac cgaatgccgc gctgagccgt gctgttgcaa aaagtaactc cccggtaacg
     3361 ttccataaac tgaccaccac gaacctcacc ggccagggcg gcaccatcaa tatgcgtgtt
     3421 cgccttgatg gcagcaatgc ctctgaccag ctggtgatta atggtggtca ggcaaccggc
     3481 aaaacctggc ttgcgtttac aaatgtcgga aacagcaacc tcggggtggc aaccaccgga
     3541 cagggtatcc gggttgtgga tgcacagaat ggcgccacca cagaagaagg tgcgtttgcc
     3601 ctgagtcgcc cgcttcaggc cggcgccttt aactacaccc tgaaccgtga cagcgatgaa
     3661 gactggtacc tgcgcagtga aaatgcttat cgtgctgaag tccccctgta tacatccatg
     3721 ttgacacagg caatggacta tgaccggatt ctggcaggct cccgcagcca tcagaccggt
     3781 gtaaacggtg aaaataacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     3841 aacaacggcg gtattgcccg tggagccacg ccggaaagca gcggcagcta tggcttcgtc
     3901 cgtctggagg gtgacctgct cagaacagag gttgccggta tgtctctgac gacaggggtg
     3961 tatggtgctg caggccattc ttccgttgat gttaaggatg atgacggttc ccgcgccggc
     4021 acggtccggg atgatgccgg cagtctgggc ggatacctga atctggtaca cacatcctcc
     4081 ggcctgtggg ctgacattgt ggcccaggga acccgtcaca gcatgaaagc gtcatcggac
     4141 aataacgact tccgcgcccg gggctggggc tggctgggct cactggaaac cggtctgccc
     4201 ttcagtatca ctgacaatct gatgctggag ccacaactgc agtacacctg gcagggactc
     4261 tccctggatg acggccagga taacgccggt tatgtgaagt tcgggcatgg cagtgcacaa
     4321 catgtgcgtg ccggtttccg tctgggcagc cacaacgata tgacctttgg tgaaggcacc
     4381 tcatcccgtg acaccctgcg cgacagtgca aaacacagtg tgagtgaact gccggtgaac
     4441 tggtgggtac agccttctgt tatccgcacc ttcagctccc ggggtgacat gagcatgggg
     4501 acagccgcag ccggcagtaa catgacgttc tcaccgtccc ggaatggcac gtcactggac
     4561 ctgcaggccg gactggaagc ccgtatccgg gaaaatatca ccctgggcgt tcaggccggt
     4621 tatgcccaca gcgtcagcgg cagcagcgct gaaggctata acggtcaggc tacgctgaat
     4681 atgactttct gattatacgg tatcccggaa atacgatttc aggaaaattc atgcagtaat
     4741 gggaaaggac gctcattgct gttcaccgca gttctgaatt attgaattta taaaggtgaa
     4801 tgatgcacga aaagaacatc gccctgcttt gtgatgaagc cgaccggctt ttgcaactga
     4861 atatcaatct gctccggcaa atggttgatg agccggatgt gttatctgac agtaagaacg
     4921 aaaacgggct gctttttgat aaacggaaag cgctgaaaag aattgaggag ctggagggcg
     4981 aacaaatcaa aaccgcccgc agggagatgg tgctggctgt ggtcggcacg atgaaggcgg
     5041 gcaaatcaac caccatcaac gccattgtgg ggcaggaaat tctgcccaac cgtaaccgcc
     5101 ccatgacctc ggtaccgaca ttgatccgcc acgttcccgg aaaaacggaa ccggtcctcc
     5161 atctggaaca tattcagccc gtccgcaatt tattaatcac actgcaggaa aaactcgcca
     5221 ccccggcagg gcagcaagtc gcacagtccc tgcagcaaac cggggatacc cgcgaactac
     5281 tggatattct ggcggatgat gtctggctta aaaatgaata ccacggggaa gacgaaatct
     5341 ttaccggact ggcatcgtta aatgacctgg ttcgtcttgc tgcggcaatg gggactgaat
     5401 ttccttttga cgaatacgca gaagtgcaga aactaccggt gatcgacgtg gcgttcagcc
     5461 acctggtggg aatggatgca tgtcagggaa cactcacact gctggatacc cccggcccta
     5521 atgaagccgg acaaccacag atggaaatga tgatgcggga tcaactacag aaagcctctg
     5581 cggtgctggc ggtgatggat tacacccaga tgaactcgaa agccgatgag gaggttcgta
     5641 aagagcttaa tgccattgct gacgtctcag ccggtcgtct gtttgtgctg gtcaataaat
     5701 tcgatgaaaa agatcgcaat ggtgatggtg cggatgccgt acgccagaag gtgccagcaa
     5761 tgctgaacag cgatgtgctg cccgcctccc gcgtttatcc cggctcatca cgtcaggcgt
     5821 acctggctaa ccgtgcgctt catgagttac gaaaaaacgg tgcgcttccg gttgatgaag
     5881 cctgggtcga tgattttatc agggaagctt tcggacctat ggtggaagag gacgactgga
     5941 aagacagcac taaggtaaat aaaaaggcag aaaaactctg gaatatatct ctgatcgatc
     6001 aactgataac ggaagtcata cagagctccc attccagagc ggcggcactg gcggttgact
     6061 ctgccgccgc aaaactgatg cagaatgcag aaaatgtcag cgaatatctg tcattacgtc
     6121 atcaggggct gcagcaaagc attcagtcac tacaggcgca tattaccagc ctgctggcgg
     6181 atatccagga gattgaggaa tgtcagaatc aggtgaccgg tgatgtcaga atggccatgg
     6241 aggacatcaa taccaaaacc ggggaattac tgacgaaggt ctgtgcctca ctggaggagg
     6301 agctgaacga ctatttcaga agcggtaaac gcaaagaaca gcaaatgctg gaggaagaaa
     6361 attcagcgca gccccgggag agaaacgcat tcgctttctt tcacgatatt ttcggaaccg
     6421 gaaatcagca tgacaggatg cgggactttg atccggacag ccccgaaata aaattcagtg
     6481 atcgcaggg
//
LOCUS       DABSYT020000174         6407 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.175, whole genome shotgun sequence.
ACCESSION   DABSYT020000174 DABSYT020000000
VERSION     DABSYT020000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.175"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(66..2099)
                     /gene="betT"
                     /locus_tag="ID725_RS23395"
                     /old_locus_tag="pgaptmp_004679"
     CDS             complement(66..2099)
                     /gene="betT"
                     /locus_tag="ID725_RS23395"
                     /old_locus_tag="pgaptmp_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGV7853119.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2228..2815
                     /gene="betI"
                     /locus_tag="ID725_RS23400"
                     /old_locus_tag="pgaptmp_004680"
     CDS             2228..2815
                     /gene="betI"
                     /locus_tag="ID725_RS23400"
                     /old_locus_tag="pgaptmp_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGV7853120.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2829..4301
                     /gene="betB"
                     /locus_tag="ID725_RS23405"
                     /old_locus_tag="pgaptmp_004681"
     CDS             2829..4301
                     /gene="betB"
                     /locus_tag="ID725_RS23405"
                     /old_locus_tag="pgaptmp_004681"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGV7853121.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4315..6003
                     /gene="betA"
                     /locus_tag="ID725_RS23410"
                     /old_locus_tag="pgaptmp_004682"
     CDS             4315..6003
                     /gene="betA"
                     /locus_tag="ID725_RS23410"
                     /old_locus_tag="pgaptmp_004682"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGV7853122.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1556 a         1781 c         1760 g         1310 t
ORIGIN      
        1 tttttacagc gagtcaatga ttcccgcgcc atatgcagac atggcgcggt tttatgcaat
       61 aacaatcacg cgtccgggaa catcacgcta tggcccggcg cttcacgatg gagatgaata
      121 aagttaaggt gccgctcgta ctggtcaaga atatcggtga tcacctgctc tttgctgtag
      181 tccatcaggt cgttgccctg gctgccttct aacaggaagg tttccagccg gtagtaggtc
      241 gatttcccgc tacgtgcgcg gtaggtaaag cccggcaccg agtattgctg cggccaaatc
      301 tgatagacaa agttttgctc ttcgcccata tgcaccaaca aatccagatg ccccaactgt
      361 tgtccctctt ccggcggcag gctttttagc tccacgtacg cgccgcgcaa ccgcaactcc
      421 tgcgccactt cttccattgc cgggtaacag accgtctcca tcatctgttt agtgtaacgc
      481 gtgcccggat aattcatcag gcgcgagaga cgttttttcc agctcaggcg atcctgaagc
      541 cccagcggtc gcggtgcggt atcgcggttg gcactttcac ggcggtaatc ttctaccttc
      601 agagatttat acaaccccgc catcacgaag aagatcacaa agctgaacgg cagccccata
      661 atcaccgtgg tgttttgcag cgcggatatc ccgttggtca tcagcatgcc gagcgtcagc
      721 aggccaatcg ccaccgacca gaagacgcgc agccagccgg gggcgtcgct gttgatatct
      781 ttaagctgcg aggtgaaatt ccccagcacc agcgccccgg agtccgccga ggtcacataa
      841 aacagcaggc cagtaatggt ggcgacggag gcgctaaagg taaacgccgg atactgcgcc
      901 agcaggctgt agaagccgcg ctccggatgg accatcgctt cctcggcaaa tgccgcgccg
      961 ccgtggatga tttcatacag cgcgctattg ccgaacaccg agagccataa cagcgtgaag
     1021 gtaaacggaa taatcaacgt gcccagcacg aactggcgaa tggtacgccc acgcgagata
     1081 cgcgccagga acaagccgac aaacggcgac catgccaccc accatgccca gaagaagagc
     1141 gtccagttat tcatccactc aaccgggcgg tcgaaggcaa aactgttgag cgtcatgccc
     1201 ataaagcgat tcacatagtc gccaacattc agcaccagtg cattaagcag gaacgaagtg
     1261 tcgcccataa acaacacgaa caggatcaat cccagcgcca gcgcgacatt aagctccgat
     1321 aacacgcgaa tgcccttatc gacaccggat gtcaccgaga tagtggcgat tatcaccgac
     1381 aaggcgatca gtgccgcttt tgccgccatc gaatcgggaa tatcaaacag tacgctcaag
     1441 ccatagttaa gctgcaccac accgataccg agcgtagtgg caataccgaa gatagtgccg
     1501 atcaccgctg caatatccac tgagtgacct atcggcccgt taatccgttt accgaagatc
     1561 gggtacagcg ccgagcggat ggtgagcggc aaattataac gatagctaaa gtatccgagc
     1621 gccatgccca tcagcgcata catcgaccag ccggttaagc cgtagtgaaa cagcgtccag
     1681 accatcgcct gacgcgcggc ctcaatcgtc tgtcccgcgc cttccggcgg ctgcatatac
     1741 tgcgttaccg gttcggctac ggaaaagaac atcagatcga taccgatccc ggcagcaaac
     1801 agcatcgccg cccaactcag caggctgaat tccggtttgg attgttctgg cccgagcttc
     1861 accgaaccaa aacgcgaaca agcgatacag accacaaaga caatatagag cgttgccgcc
     1921 agcagatagt accaaccgaa ggttttagaa acccagtcca gcgtgcggcc aatccacagg
     1981 gccgagaagt cgcgaaacag gatcgttgtc agggaaaaca acaaaatcag tccggcggag
     2041 gtgtaaaaca ccaccggatt gattttgtcc ttttccctgc tgtgtgaaag gtctgtcatc
     2101 cagtatcccc actgttattg ttaccattta aaatcaaatt cgtaacaatt aagacacatt
     2161 ttatattgaa cgtccaatca ataaccgctt taatagataa acaacgctga tgaatggagt
     2221 ggcaaaaatg cccaaattgg ggatgcagtc gatccggcgc agacaactga tcgacgccac
     2281 actggaagca ataaatgaag tgggcatgca cgatgcaacg atcgcgcaga tcgcccgccg
     2341 tgcaggcgtt tctacgggga tcatcagcca ctatttcagg gacaaaaatg gtctgctgga
     2401 agcaaccatg cgcgatatca ccagtcagct gcgtgacgcg gttttgaatc gattacatgc
     2461 acttccgcag ggcagtgcag agcagcgatt acaggcgatt gttggcggaa acttcgatga
     2521 aacgcaggtg agcagtgcgg cgatgaaagc ctggctggcg ttctgggcca gcagtatgca
     2581 tcagccgatg ctctatcgtt tacagcaggt cagcagccgc cgcttgctgt cgaatctggt
     2641 gagcgagttt cgccgcgaat tgccgcgcca ccaggcacag gaagcgggct acggccttgc
     2701 cgcgctgatt gacggattat ggctgcgcgc ggcactgagc ggcaaaccgc tggataaacc
     2761 tctcgcacac tcgctgaccc gtcactttat tactcagcat ttacccaccg attaaccgag
     2821 gagacgtgat gtcccgaatg gcagaacagc agctttatat acatggtggt tatacctccg
     2881 ccaccagcgg tcgcaccttc gagaccatta acccggccaa cggtaacgtg ctggcgaccg
     2941 tgcaggccgc cgggcgcgag gatgtcgatc gcgccgtgaa gagtgcccaa caggggcaaa
     3001 aaatctgggc ggcgatgacc gctatggaac gctcgcgtat tctgcgtcgg gccgtcgata
     3061 ttctgcgcga gcgcaatgat gaactcgcca aactggaaac cctcgacacc ggaaaagcgt
     3121 attcggaaac ctcaaccgtc gatatcgtta ccggtgcgga cgtgctggag tactacgccg
     3181 ggctgatccc gtcgctggaa ggcagccaga tcccgttgcg tgagacatca tttgtctaca
     3241 ctcgccgcga accgctgggc gtggtggcag ggattggcgc atggaactac ccgatccaga
     3301 ttgccctgtg gaaatccgcc ccggcgctgg cggcgggtaa tgcgatgatt ttcaaaccga
     3361 gcgaagtcac tccgcttacc gcgttaaagc tggctgaaat ttacagcgaa gcgggcctgc
     3421 cggacggtgt atttaacgtg ttgccgggcg tgggcgcgga gaccgggcaa tatctgaccg
     3481 agcatccggg cattgccaaa gtgtcgttta ccggcggcgt cgccagcggc aaaaaagtga
     3541 tggctaactc ggcagcctct tccctgaaag aagtgaccat ggaactgggc ggtaaatcac
     3601 cgctgatcgt tttcgacgat gcggatctcg atctcgccgc cgatatcgcc atgatggcaa
     3661 acttcttcag ctccggtcag gtgtgtacca atggcacccg cgtcttcgtt ccggcgaaat
     3721 gcaacgccgc atttgagcag aaaattctgg cgcgcgttga gcgcattcgc gcgggcgacg
     3781 ttttcgatcc gcaaactaac ttcggcccgc tggtcagctt cccgcatcgc gataacgtgc
     3841 tgcgctatat cgccaaaggc aaagaggaag gcgcgcgcgt actgtgcggc ggcgatgtac
     3901 tgaaaggcga tggcctcgat aacggcgcat gggttgcacc gaccgtgttc accgattgca
     3961 gcgacgagat gaccatcgtg cgtgaagaga tcttcgggcc ggtgatgtcc attctgacct
     4021 acgagtcgga agacgaagtc attcgccgcg ccaatgatac cgactacggc ctggcggcgg
     4081 gtatcgtgac ggcggacctg aaccgcgcgc atcgcgtcat tcatcagctg gaagcgggta
     4141 tttgctggat caacacctgg ggcgaatccc cggcagagat gcccgttggc ggctacaaac
     4201 actccggtat tggccgcgag aacggcgtga tgacgctcca gagttacacc caggtgaagt
     4261 ccatccaggt tgagatggct aaattccagt ccatattcta accaggaggt ttatttgcaa
     4321 tttgactaca tcattattgg tgccggctca gccggcaacg ttctcgctac ccgtctgact
     4381 gaagatccga atacctccgt gctgctgctt gaagcgggcg gcccggacta tcgctttgac
     4441 ttccgcaccc agatgcccgc tgccctggca ttcccgctac agggtaaacg ctacaactgg
     4501 gcttacgaga cggaacctga accgtttatg aataaccgtc gcatggagtg cggacgcggt
     4561 aaaggcctgg gtggatcgtc gctgatcaac ggcatgtgct atatccgtgg caacgcgatg
     4621 gatctcgaca actgggcaaa agaacccggt ctggagaact ggagttatct cgattgcctg
     4681 ccctactacc gcaaggccga gacccgcgac gtgggcgaga acgactacca cggcggcgac
     4741 ggcccggtga gcgtcaccac ctccaaaccc ggcgtcaatc cgctgtttga agcgatgatt
     4801 gaagcgggcg tgcaggcggg ctacccgcgc acggacgatc tcaacggtta tcagcaggaa
     4861 ggtttcggcc cgatggatcg caccgtcacg ccgcagggcc gccgcgccag caccgcgcgc
     4921 ggttatctcg atcaggccaa atcgcgccca aacctaacca ttcgtactca cgccatgacc
     4981 gatcacatta tttttgactg taaacgcgcg gtgggcgtcg agtggctgga aggcgacagc
     5041 accattccga cccgcgcgac ggcgaacaaa gaagtgctgt tatgtgcagg cgcgattgcc
     5101 tcaccgcaga tcctgcaacg ctctggcgtc ggcaacgctg aactgttggc cgagtttgat
     5161 attccgctgg tgcatgattt acccggcgtc ggtgaaaatc ttcaggatca tctggagatg
     5221 tatctgcaat atgagtgcaa agaaccggtt tccctctacc ctgccctgca gtggtggaat
     5281 cagccgaaaa tcggtgcgga gtggctgttt ggcggcaccg gcgttggtgc cagcaaccac
     5341 tttgaagcag gcggatttat tcgcagccga gaggaatttg cgtggccgaa tattcagtat
     5401 cacttcctgc cggtagcgat taactataac ggctcgaatg cagtgaaaga gcacggcttc
     5461 cagtgccacg tcggctcgat gcgctcgcca agccgtgggc atgtgcggat taaatcccgc
     5521 gacccgcacc agcatccggc aattctgttt aactacatgt cgcacgaaca ggactggcag
     5581 gaattccgcg acgcaattcg catcacccgc gagatcatgc atcaacccgc gctggatcag
     5641 tatcgtggcc gcgaaatcag ccccggcacg gaatgtcaga cggatgagca gctcgatgag
     5701 tttgtgcgta accacgccga aaccgccttc catccgtgcg gtacctgcaa aatgggctac
     5761 gacgagatgt ccgtggttga cggcgaaggc cgcgtgcatg ggctggaagg cctgcgcgtg
     5821 gtggatgcgt caattatgcc gcagattatc accgggaatt tgaacgccac gacgattatg
     5881 attggcgaga aaatggcgga tatgattcgc gggaaggaag cgttgccgag gagcacggcg
     5941 ggatattttg tggcaaatgg gatgccagta agagcgaaaa aaatgagtcg tgatttgaac
     6001 tgactcagga accgatcaga ttgagagtta ccgttccagg tatggggaca gaatcgttat
     6061 ataaaacata ggggtaactc tcatgcttca tagtgacaac ctcgccagca aattctaagc
     6121 tgactcgttc aatctcgctg atgaaggtac tatgaatgca ctgataaacc gcaggtgaaa
     6181 tgccactcag cttaagaatt acgccgctag tgttcctggc tatggtgact tgttcactcc
     6241 cgctcacggt tagcatatac gaaaagggtt cttgtaacga ggacaaaaat aagtcagacc
     6301 aaaacaacgt ggagctttaa acacaattcg tataatttat tagcagtgat ttatttttac
     6361 tcttctttaa ttaatcatca tgcttaaata ttttttagta caaggtt
//
LOCUS       DABSYT020000175         6212 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.176, whole genome shotgun sequence.
ACCESSION   DABSYT020000175 DABSYT020000000
VERSION     DABSYT020000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.176"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..167)
                     /locus_tag="ID725_RS23415"
                     /old_locus_tag="pgaptmp_004683"
     CDS             complement(<1..167)
                     /locus_tag="ID725_RS23415"
                     /old_locus_tag="pgaptmp_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853123.1"
                     /translation="MKTWGCGGLELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKP
                     HERKRAVAHLTL"
     gene            complement(109..303)
                     /locus_tag="ID725_RS23420"
                     /old_locus_tag="pgaptmp_004684"
     CDS             complement(109..303)
                     /locus_tag="ID725_RS23420"
                     /old_locus_tag="pgaptmp_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853124.1"
                     /translation="MRALCAVNGSHPENLRFWLKACPVVLRERFHQQCKCRFNWSTQQ
                     LCENMGLRRFGTMEERNRLQ"
     gene            complement(324..605)
                     /locus_tag="ID725_RS23425"
                     /old_locus_tag="pgaptmp_004685"
     CDS             complement(324..605)
                     /locus_tag="ID725_RS23425"
                     /old_locus_tag="pgaptmp_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853125.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(1144..1230)
                     /locus_tag="ID725_RS23430"
                     /old_locus_tag="pgaptmp_004686"
     tRNA            complement(1144..1230)
                     /locus_tag="ID725_RS23430"
                     /old_locus_tag="pgaptmp_004686"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1194..1196),aa:Leu,seq:taa)
     gene            complement(1243..1316)
                     /locus_tag="ID725_RS23435"
                     /old_locus_tag="pgaptmp_004687"
     tRNA            complement(1243..1316)
                     /locus_tag="ID725_RS23435"
                     /old_locus_tag="pgaptmp_004687"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1282..1284),aa:Cys,seq:gca)
     gene            complement(1370..1445)
                     /locus_tag="ID725_RS23440"
                     /old_locus_tag="pgaptmp_004688"
     tRNA            complement(1370..1445)
                     /locus_tag="ID725_RS23440"
                     /old_locus_tag="pgaptmp_004688"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1410..1412),aa:Gly,seq:gcc)
     gene            complement(1597..2145)
                     /gene="pgsA"
                     /locus_tag="ID725_RS23445"
                     /old_locus_tag="pgaptmp_004689"
     CDS             complement(1597..2145)
                     /gene="pgsA"
                     /locus_tag="ID725_RS23445"
                     /old_locus_tag="pgaptmp_004689"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGV7853126.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(2202..4034)
                     /gene="uvrC"
                     /locus_tag="ID725_RS23450"
                     /old_locus_tag="pgaptmp_004690"
     CDS             complement(2202..4034)
                     /gene="uvrC"
                     /locus_tag="ID725_RS23450"
                     /old_locus_tag="pgaptmp_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGV7853127.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(4031..4687)
                     /gene="uvrY"
                     /locus_tag="ID725_RS23455"
                     /old_locus_tag="pgaptmp_004691"
     CDS             complement(4031..4687)
                     /gene="uvrY"
                     /locus_tag="ID725_RS23455"
                     /old_locus_tag="pgaptmp_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGV7853128.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4829..4945
                     /locus_tag="ID725_RS23460"
                     /old_locus_tag="pgaptmp_004692"
     CDS             4829..4945
                     /locus_tag="ID725_RS23460"
                     /old_locus_tag="pgaptmp_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGV7853129.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            4983..5159
                     /gene="yecU"
                     /locus_tag="ID725_RS23465"
                     /old_locus_tag="pgaptmp_004693"
     CDS             4983..5159
                     /gene="yecU"
                     /locus_tag="ID725_RS23465"
                     /old_locus_tag="pgaptmp_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGV7853130.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            5146..5370
                     /gene="yecF"
                     /locus_tag="ID725_RS23470"
                     /old_locus_tag="pgaptmp_004694"
     CDS             5146..5370
                     /gene="yecF"
                     /locus_tag="ID725_RS23470"
                     /old_locus_tag="pgaptmp_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGV7853131.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDEPQAAVFSKTVKQIKQAYRQ"
     gene            complement(5438..6160)
                     /gene="sdiA"
                     /locus_tag="ID725_RS23475"
                     /old_locus_tag="pgaptmp_004695"
     CDS             complement(5438..6160)
                     /gene="sdiA"
                     /locus_tag="ID725_RS23475"
                     /old_locus_tag="pgaptmp_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGV7853132.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         1664 a         1596 c         1374 g         1578 t
ORIGIN      
        1 agtgtcaggt gagctacagc ccgcttacgc tcatggggtt ttatgccgcc agtatccctt
       61 aacatagtga agatcgttcc gggttttgaa cccaggatat tcgctatttc actgaagcct
      121 gttccgttct tccatagttc caaacctccg caaccccatg ttttcacata actgttgcgt
      181 tgaccaattg aatctacact tacattgttg atgaaatcgc tcacgaagga ccaccggaca
      241 ggctttcagc cagaagcgca gattttcggg atgtgaaccg ttaacggcac ataatgctcg
      301 cacaacaaac ctgttaaaag agattactca gcattctcct gcttccattg ccggatcatt
      361 tcatcggtaa catcaccctc ataacatacc acgtcatacc ctccattacg gctatatgca
      421 ctgcgtccac cacatctact accattcctt gcatgattat aaggacacgc acaattgcct
      481 ggataagatt caatggattc tttaattatt tcttttttga tctgagcatc agactttcct
      541 gttgctgcat atccactaaa agatactaaa caaaggcaga ttgccattaa taaaccacat
      601 cgcataccga tacactcaat tactaaccaa tcatagccca catcataaaa tcgagatgag
      661 gagcacaata cctttaacaa gaaggtgtca taacgataat atgtcgtgca attcatcaaa
      721 aaaaagatct atatcaaaga gatagaactc ttatcagtgc ttcagacatc ggcattcgag
      781 gcagcagaaa aacagtaaat ctgacgaatt tgccttttcc caagatgttg tcagattaaa
      841 aagatagatt agcgcaggta cctgttggtg gattgcagga ggaaaaataa agtcgagtgg
      901 aaaaatccca gaaaaatttt tgtttaacag aggtcctggt tcactatgcg aaatacggag
      961 gagctgcaga agaattctgc ttcatcctta gagtttaaac agcacgtgca ctaccattgt
     1021 ttctgcatat cgccacacca tcgccactct accgccattg cacaaaatac aaatacgaaa
     1081 aaaccacccg aaggtggttt cacgacactg cttattgctt tgattttatt cttagctttc
     1141 ccatggtacc cggagcggga cttgaacccg cacagcgcga acgccgaggg attttaaatc
     1201 ccttgtgtct accgattcca ccatccgggc tcgggaagaa agtggaggcg cgttccggag
     1261 tcgaaccgga ctagacggat ttgcaatccg ctacataacc gctttgttaa cgcgccaaat
     1321 tcttcaggcc tttcagccag acatccgctg acgccgatgt cttttaaact ggagcgggaa
     1381 acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt
     1441 cccgcattca tcaagcaatc agttaatcac ttgattttat tatcgtctgg caatcagtgc
     1501 cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc
     1561 acttttgatc gtttgctgaa aattgcgccg aaacgatcac tgatcaagca aatctgcacg
     1621 cgcagcgctc aaatattgca acattgacca cagagtcagt accgcagcca caaagaaaag
     1681 tgcaataccg gcgtactcaa cccaaatgtt cggacgccac agcagccatg ccaacgccac
     1741 catctgggca gtggttttca ctttcccaat ccaggagaca gccacgctac tgcgtttacc
     1801 caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga tcatcgttgc
     1861 cgccggtaag gtcacccacc agctgtggta atgctcggtt accagcacca tggcgatagc
     1921 cacgagaact ttatctgcta cagggtcaag gaaagcacca aaccgggtac tctggttcca
     1981 gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc
     2041 ggcggcaaac ggcgaccaag tgacaggcag ataaaagacc aatacaaaga atgggataag
     2101 gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta
     2161 tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag
     2221 aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg
     2281 gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg gcgttttggc
     2341 ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg
     2401 tgcccgccaa tcgcgtgatc gtgtgattca tcgcgaatat gctggataac atgcagcgcg
     2461 ggagaatctg gcggcaaact gaatccctca ccttccggct caaagaacag cgtttccagc
     2521 ccagccttac gatctgctcc tttggcaaca ccaagtagca gcggatgatt tttatcccat
     2581 gagacatcca gttcggcgaa gacatttttc gcctgcgcaa gctggccttt gccgccgtcg
     2641 ataagtatca catccgggat cttactgtcg tcgatggctt taccataacg ccgacgcagc
     2701 acctgattca tcgccgcata atcatcgccc ggcgtgatgc cagtaatgtt atagcgccga
     2761 tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttca
     2821 cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa tttcaacacg
     2881 ctggcaagtg ctgtaagccg ctggtgaacg gtagattgct gcgaaagttt gctggttaag
     2941 gccgtcgccg cattggtgcg cgcgagtttc aggtaacgcg ccctatcgcc acgaggtttg
     3001 gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta
     3061 tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt
     3121 aaatagaact ggcctacgaa ggtttctacc acctcgctca gttccgtacc gccaggcact
     3181 ttcgggaaat agctgcggct gccgagcact ttgccctgac gaatgaacaa tacgtggaca
     3241 caagccatac ccgcatcgaa cgccacacca ataacgtcga ggtcgtcgcc ggtattggaa
     3301 acgaattgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acgtgccgct
     3361 tcttcaaact ccagattctg actggcagtt tccatacggc taatgagttg cgtaagcacc
     3421 tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc gtattcttct
     3481 tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctgata ttgcagacac
     3541 ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa aatcttttgc
     3601 agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa atattcacct
     3661 ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt gggtatcgcc actcaggaag
     3721 ataaaaggat atgatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg
     3781 atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gagtaaccgt tacatcaatt
     3841 tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct acggaaatag
     3901 ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca
     3961 tcgtacatgc gataaacgcc aggctggctg gttacggttt ttaaaaacgc ttttgcgtca
     4021 aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca
     4081 ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc
     4141 tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct
     4201 tcgtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg
     4261 ttttttctgg ttcgatctgg cttaatgcca tttgttgagc gatgtcagaa gcaatgtaac
     4321 gctgcccaga atagacagaa cggatcgcac tcacgacttc ctgcggagcc gcgcctttgc
     4381 tgaggtagcc cgcagcaccg gcctgcatga ctttcgctgg taaagggttt tctgtatgga
     4441 cggtaagcat gatgattttt acatcagctg tggaacgcgc gattttacgc gtcgcctcaa
     4501 gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgcccggc
     4561 accacttaac ggcgtcttca ccgcacgatg cctcaccgac gacttttata ccctttatat
     4621 cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt
     4681 tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt cattcgtata
     4741 ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc
     4801 gtttttgtca attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttaccgga
     4861 tcgtttcaca gtaagccctg cgacgaaatg tgacaaaaat tacctttatt cagcaaaaat
     4921 gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg caaaaaacat
     4981 tcatgataaa aatatttatt ggtcattata ttaacgcatt taatagcacc gttgacggca
     5041 cgctcaaaaa acaaccactg ctatttttag caaagcttat ggtatactcc gccgccttaa
     5101 catttttcac cgcaaatttt cattgcaaca tgacgaggaa aataaatgag tacgcctgat
     5161 ttttctactg ccgagaataa tcaagaactg gcaaatgaag tctcctgcct gaaagcgatg
     5221 ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat gttaaagatg
     5281 gaacaacagc ttgcgctgat cgaagacgaa ccccaggctg cagtattttc caaaacggtt
     5341 aagcaaatta aacaagccta ccgtcagtaa tataaaaccg gctgatagcg tgcctttcag
     5401 ccggtttttg catctggcac gcaggacaga aaagagatca aattaagcca gtagcggccg
     5461 cgtaacaggc aacctgggtc ttatttggtg cattaatttt tttctgcatg tttttctggt
     5521 ggaaattgac cgtattctca gagattgaca aaatcatcgc tatctctgct gatgtcttcc
     5581 cttccgccgt ccacctcaga atttcttttt cgcgcttgct gaaattcatc tctggcgtca
     5641 tcactatttc atcatttaaa cgcatcagag ccatcagact ttcgcgcacc agtaactgca
     5701 tttttaattg cagttcatca ctaagaatgg gtatttcgcg cgcgctgcaa cgggaaaagg
     5761 acaaaaagcc cagcgcccgg tttggcagca ttaaatactg agtgacaccg cggcgtaaac
     5821 catgtgcgcg cgcggcttcc cataacggct gtgcttcgtt gaataagtca tcattccaca
     5881 ttagatggcc ctgactaaag ttttcagggt tcagcaccgg atcaattgcg agaaagtttt
     5941 ttgcctgata ataactaacc cacgcctcag ggtaattggt gtagaaagcc actttaggtc
     6001 gagtgaatgg taccgggtgg cggacacata acgaatagta atcgtactcc agctgctgag
     6061 cctgaagctc aatttcatgg tagacctctt ctgcggtctc catcctctga aaacgcaaca
     6121 gcatcgtgcg acgccagctg aaaaaatccg tatcctgcat agtaaaccgc aacgcccctg
     6181 agagtgagta tcatttataa tgataattca aa
//
LOCUS       DABSYT020000176         6179 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.177, whole genome shotgun sequence.
ACCESSION   DABSYT020000176 DABSYT020000000
VERSION     DABSYT020000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.177"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            350..868
                     /locus_tag="ID725_RS23480"
                     /old_locus_tag="pgaptmp_004696"
     CDS             350..868
                     /locus_tag="ID725_RS23480"
                     /old_locus_tag="pgaptmp_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGV7853133.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1078..3219
                     /locus_tag="ID725_RS23485"
                     /old_locus_tag="pgaptmp_004697"
     CDS             1078..3219
                     /locus_tag="ID725_RS23485"
                     /old_locus_tag="pgaptmp_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGV7853134.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3295..>6179
                     /locus_tag="ID725_RS23490"
                     /old_locus_tag="pgaptmp_004698"
     CDS             3295..>6179
                     /locus_tag="ID725_RS23490"
                     /old_locus_tag="pgaptmp_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGV7853135.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDR"
BASE COUNT         1435 a         1616 c         1966 g         1162 t
ORIGIN      
        1 gattaagtgg gtatttttaa ggaatatctc aaaaattatc aaaatgataa ttcagaaagg
       61 ataaatacaa tatttattca tgattcagct ttggccattt tatggaggca atacggactt
      121 tgaacagttg ggtatggcta tccagatata tattattaag ttattgctga ataagagatt
      181 taatgatttt aaaaatagtt tttacgacaa ccgtaaattc tagctcggtt tgtatgtaaa
      241 aaatactgaa aaatgatgcg gttccgtgaa gtgagctgca tctgcctgtc tgggattaac
      301 ggcaggtcat tgtaagtagt aattaaccac gttaacaagg agtttacaaa tgccaacacc
      361 gtgttatatc tccatcactg gtcagaccca gggaaatatt actgcaggtg catttaccgc
      421 tgattctgtc ggcaacatct atgtgcaggg gcacgaagac gagatgctgg tgcaggaatt
      481 cctgcataac gttactgtcc cgaccgatcc tcagtccggt cagcctgcgg gtcagcgtgc
      541 tcacaagcct ttcattttta ccgtggcgct gaacaaagcg gttccgctga tgtacaacgc
      601 actggcctcc ggcgaaatgc tgccaaccac tgaacttcac tggtggcgta cctctgtgga
      661 aggcaaacag gagcattatt tcactacccg cctgactgat tcgacgattg tcgatatgaa
      721 actgcatatg ccgcattgcc aggacccggc gaaacgcgag ttcacccagt tgctcgaagt
      781 ttcactggcc taccgcaaaa ttgaatggga acacgtcaaa tctggcacct ccggtgccga
      841 cgactggcgc gcaccgctgg aagcataagt taagccaaca gcatccggct atcaggccgg
      901 atgctgtttt tgtattacgc cgtagaaaga aaggttgtca gtaaggggtt acgttcacgg
      961 gagatggcgg ctgccggaaa cttcggcggt agcgtgcctg aacgcaagcg tagcactgta
     1021 ctgtcacagc cgctttaaca gcgaactgtc tgtttttcct taaccccgga ggttgctatg
     1081 tcaaccggat tacgtttcac gctggaagtg gacggcctgc caccggatgc ttttgcggtg
     1141 gtctcctttc atctgaacca gtcactctct tcgctttttt ccctcgctct ctctctggtc
     1201 agccagcagt ttctctccct tgaattccag cagatcctcg acaaaatggc ctacctgacg
     1261 atatggcagg gcgatgacgt acagcgccgg gtgaaaggtg tggtgacctg gtttgaactg
     1321 ggggagaacg acaaaaacca gaagctgtac agcatgaagg tatgcccgcc gctgtggcgc
     1381 acagggctgc gccagaactt ccgtatcttc cagaatgagg acatcgcaag catcctcggc
     1441 acgatattgc aggaaaacgg ggtgaccgag tggagcccgc tgttcagcga gccacatcct
     1501 tcccgtgagt tttgtgtcca gtacggtgag actgattacg atttcctgtg ccggatggcg
     1561 gcggaggaag gcatcttctt ttatgaggag cacgcgcaaa aaagtaccga ccagagcctg
     1621 gtcctgtgcg ataccgtgct ttatctgccg gagtcctttg agatcccctg gaatccgaac
     1681 acccgtaccg aggtaagcac cctctgcatc agccagtttc gctacagcgc acaaatccgc
     1741 ccttcttccg tggtgaccaa agactacacc tttaaacgcc ccggctgggc cggacgtttt
     1801 gaccaggaag gccagcacca ggattaccag cgcacacagt atgaagtgta tgactatccc
     1861 ggacgtttca agggcgccca cgggcagaac tttgcccgct ggcagatgga cggctggcga
     1921 aacaacgcag aagtggcgcg cggaacaagc cgttcgccgg agatatggcc gggacggcga
     1981 attgtgctga cggggcatcc gcaggcgaac ctgaaccggg aatggcaggt ggtggcaagt
     2041 gaactgcacg gtgagcagcc gcaggcggtg ccgggacgca ggggttcagg caccacgctg
     2101 gataaccatt ttgcggtaat accggcagac agaacatggc gaccacagcc gttgctgaaa
     2161 ccgctggtgg acggcccgca gagcgccgtc gtgacgggac cggcaggcga ggaaatcttc
     2221 tgtgatgaac atggccgcgt gcgggtgaaa tttaactggg accgctataa cccgtcaaac
     2281 caggacagtt catgctggat ccgtgtggca caggcgtggg caggcaccgg gtttggtaac
     2341 ctggcgatac cgcgtgtggg tcaggaggtc attgtggact tcctcaacgg cgatccggac
     2401 cagccgatca ttatggggcg tacctaccac caggaaaacc gcacacccgg tagcctgccg
     2461 gggacgaaga cgcagatgac cattcgttcg aaaacctata agggcagcgg gtttaatgaa
     2521 ctgaagtttg acgatgcgac agggaaagaa caggtctaca tccacgcgca gaagaacatg
     2581 aacaccgagg tgctgaataa ccgcaccacg gatgtgataa acaaccatgc cgaaaaaata
     2641 ggtaacaacc aggcgatcac cgttaccaat aaccagatcc tgaacattgg cgttaatcag
     2701 atacagacgg ttggtgtcaa ccaggtggaa acggtaggca gtaaccagat tatcaaagtg
     2761 ggatcaaacc aggttgaaaa ggtggggatc attcgtgcgc tgacggtggg tgtggcgtat
     2821 cagacgacgg taggcggcat tatgaatacc tcggtggcgt tgttgcagtc ctcacaggta
     2881 gggctgcata aatcactgat ggtgggaatg ggctacagcg tcaatgtagg gaataacgtc
     2941 accttctcgg tgggcaagac gatgaaggaa aacaccggac aaacagcagt ttattctgcc
     3001 ggtgagcatc ttgaactctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggaagc
     3061 atatttctta atggtacgca cattcatctg gaaggggagt cggatgtgaa cggtgatgcg
     3121 ccggtgatta actggaactg tggtgccaca caacctgtac cggatgcgcc tgtgccgaaa
     3181 gatttacccc ccggaatgcc ggatatgcgg caattttgag tcaggtgcaa aaaatcaaaa
     3241 cggaactggt gcagtgacca gataaaccat attaaaagca gaaggaattg attcatggga
     3301 ggaaaaccgg cggcgcggca gggtgacatg acccgcaagg gactggatat tgtgcagggt
     3361 tcagcagggg tgctgatagg tgcgccgacg ggcgtggcct gctcggtgtg tccgggaggg
     3421 attacctatg ctaacccggt gaacccggtg ctgggtgcga aggtgctgcc gggcgagacg
     3481 gaccttgcgc tgcccggccc gctgccgttt attctttccc gcgcctacag cagctaccgg
     3541 accagaacac ccgcgccggt gggggtgttt ggtcccggct ggaaagcgcc cttcgatatc
     3601 cgcttacaga tacgcgatga aggcctgata ctcaacgaca acggcggcag gagcattcac
     3661 tttgagccgc tgtttcccgg cgagataagc tacagccgca gcgagtcgtt ctggctggcg
     3721 cggggcgggg tggcggagca gcacagttcg cagccgctaa gcgcgctctg gcaggtgctg
     3781 ccggaagatg ttcgcctgag tccgcatatg tacctggcga caaacagcct tcaggggccg
     3841 tggtggatac tcaactggcc ggagcgggtg ccgggggcgg acgaggtgct gccgccggag
     3901 ccgcccgcat accgggtgct gacgggcgtg gtggatggct tcgggcggac gctggccttt
     3961 caccgggcgg cggagggtga tgtggcgggc gcggtgacgg gggtgacgga cggcgcgggc
     4021 cgccgttttc acctggtgct gaccacacag gcacagcggg cggaagtatt ccgtaaacag
     4081 cgcgccacgt ctttatcttc ccctgccggt ccccgttctg cttcctcttc tctggttttc
     4141 cccgacacgc tgcccgccgg tacagaatac ggtgccgata acggtatccg gctggaggcg
     4201 gtatggctga cacacgaccc ggcatacccg gatgaactgc ccgccgcgcc gctggcgcgc
     4261 tacacgtaca cggccagcgg agaactgcgg gcggtgtatg accgcagcgg gacgcaggtg
     4321 cgcgggtttg cttatgatgc ggagcacgcc gggcggatgg tggcgcacca ttatgcgggt
     4381 aggccggaga gccgctaccg gtatgatgat accggccggg tgacggagct ggtcaacccg
     4441 gaggggctgg actaccgctt tgagtacggg caggaccgtg tgaccatcac ggacagcctg
     4501 aaccggcggg aggtgctgta cacggaaggc gagggtggcc tgaaacgtgt tgtgaagaag
     4561 gaacatgcgg acgggagcat cacccgcagc gagtatgatg aggcggggag gctgaaggca
     4621 cagacggatg cggcgggacg gcggacggag tacagcctgc atatggcgtc gggtgcggtg
     4681 acagcggtga cggggccgga cggcaggacg gtgcggtatg gctataacag ccagcggcag
     4741 gtgacgtcag tgacgtaccc ggacgggctg cgcagcagcc gggagtatga tgagaaggga
     4801 aggctggcgg cggagacctc gcgcagcgga gagacgacgc ggtacagcta tgatgacccg
     4861 gcgagtgagc tgccgacagg gatacaggac gcgacgggca gtacaaaaca gatggcatgg
     4921 agccgttacg gtcagctgct gacctttacg gactgctcgg ggtacacgac gcggtatgag
     4981 tatgaccggt acggtcaaca aatcgccgtt caccgggaag aaggcatcag cacttacagc
     5041 agttataacc cgcgtggcca actggtcagt cagaaggatg cgcaggggcg tgaaacccgt
     5101 tatgagtaca gcgccgcagg cgacctcacc gctatcgttg ccccggacgg cagccgcagt
     5161 gagatacagt atgatgcgtg gggaaaggcc gtcagcacca cgcagggcgg tctgacgcgc
     5221 agcatggggt atgacgctgc cgggcgcatc accgtgctga ccaacgagaa cggcagccag
     5281 tccacgttcc ggtatgaccc ggtggacagg ctgactgaac agcgcggttt tgacggccgg
     5341 acgcaacgtt accactatga cctgaccgga aaactcacgc agagtgaaga cgaggggctt
     5401 gtcaccctct ggcactacga tgcgtcggac cgcatcacgc accggacggt gaacggcgac
     5461 ccggcagagc agtggcagta tgatgagcac gggtggctaa ccaccctcag ccataccagt
     5521 gaaggccacc gggtgtcggt ccactacggc tatgacgata aaggccgcct gacgggtgaa
     5581 cggcagacgg tggagaaccc ggagacgggg gagatgctgt gggagcatga gacggggcac
     5641 gcgtacagcg aacaggggct ggcgacccgt caggagccgg acggtctgcc gccggtagag
     5701 tggctgacgt atggcagcgg ttatcttgcg gggatgaagc tgggcggaac gccactggtc
     5761 gagtacatgc gggaccggct gcaccgtgag acggcccgca gcttcggcgg ggaggcatat
     5821 gaacttgcca ccgcctggaa taccagcggc cagctccgga gcaggcacct gaaccttccg
     5881 cagcttgacc gtgactacga ctggaacgac aacggacagc taatccgcat cagcggcccg
     5941 caggagagcc gggagtaccg ttacagtgac acgggaaggc tgacgggcgt ccacaccacg
     6001 gcagcgaacc tggatatcga tatcccgtat gcaacggacc cggcaggaaa ccggctgccg
     6061 gacccggaac tgcatccgga cagcacgctc acggcgtggc cggataaccg catcgcggaa
     6121 gatgcgcact atatctatcg ctacgatgaa tacggcaggc tggcggagaa gacggaccg
//
LOCUS       DABSYT020000177         6072 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.178, whole genome shotgun sequence.
ACCESSION   DABSYT020000177 DABSYT020000000
VERSION     DABSYT020000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.178"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..253
                     /locus_tag="ID725_RS23495"
                     /old_locus_tag="pgaptmp_004699"
     CDS             <1..253
                     /locus_tag="ID725_RS23495"
                     /old_locus_tag="pgaptmp_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGV7853136.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..843
                     /locus_tag="ID725_RS23500"
                     /old_locus_tag="pgaptmp_004700"
     CDS             265..843
                     /locus_tag="ID725_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853137.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            840..1235
                     /locus_tag="ID725_RS23505"
                     /old_locus_tag="pgaptmp_004701"
     CDS             840..1235
                     /locus_tag="ID725_RS23505"
                     /old_locus_tag="pgaptmp_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGV7853138.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1243..1983
                     /locus_tag="ID725_RS23510"
                     /old_locus_tag="pgaptmp_004702"
     CDS             1243..1983
                     /locus_tag="ID725_RS23510"
                     /old_locus_tag="pgaptmp_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853139.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1999..2421
                     /locus_tag="ID725_RS23515"
                     /old_locus_tag="pgaptmp_004703"
     CDS             1999..2421
                     /locus_tag="ID725_RS23515"
                     /old_locus_tag="pgaptmp_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGV7853140.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2403..2837
                     /locus_tag="ID725_RS23520"
                     /old_locus_tag="pgaptmp_004704"
     CDS             2403..2837
                     /locus_tag="ID725_RS23520"
                     /old_locus_tag="pgaptmp_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGV7853141.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2830..5379
                     /locus_tag="ID725_RS23525"
                     /old_locus_tag="pgaptmp_004705"
     CDS             2830..5379
                     /locus_tag="ID725_RS23525"
                     /old_locus_tag="pgaptmp_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGV7853142.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5376..5705
                     /locus_tag="ID725_RS23530"
                     /old_locus_tag="pgaptmp_004706"
     CDS             5376..5705
                     /locus_tag="ID725_RS23530"
                     /old_locus_tag="pgaptmp_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853143.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5705..>6072
                     /locus_tag="ID725_RS23535"
                     /old_locus_tag="pgaptmp_004707"
     CDS             5705..>6072
                     /locus_tag="ID725_RS23535"
                     /old_locus_tag="pgaptmp_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGV7853144.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         1410 a         1487 c         1986 g         1189 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcgtt
      361 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaag agtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggccaga
      481 atcaaggtta accgggggga tttgcccgta atcaagctgg gtaacgcgcg ggttgtcctg
      541 tcccgacgca ggcgtcgtaa aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg
      601 cttgtggtgg gaaaccgtcg tattcccggc gcgtttattc agcaactgaa aaatggccgg
      661 tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt ggtgaaaatc
      721 ccgatggcgg tgccgctgac cacggcgttt aaacagaata ttgagcggat acggcgtgaa
      781 cgtcttccga aagagctggg ctatgcgctg cagcatcaac tgagaatggt aataaagcga
      841 tgaaacatac tgaactccgt gcagccgtac tggatgcact ggagaagcat gacaccgggg
      901 cgacgttttt tgatggtcgc cccgctgttt ttgatgaggc ggattttccg gcagttgccg
      961 tttatctcac cggcgctgaa tacacgggcg aagagctgga cagcgatacc tggcaggcgg
     1021 agctgcatat cgaagttttc ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga
     1081 tggagtcccg gatttatccg gtgatgagcg atatcccggc actatcagat ttgatcacca
     1141 gtatggtggc cagtggctat gactaccggc gcgacgatga tgcgggcctg tggagttcag
     1201 ccgatctgac ttatgtcatt acctatgaaa tgtgaggacg atatgccaac accaaatcct
     1261 ctggcaccgg tgaaaggggc cgggaccaca ctgtgggttt ataacgggaa cggcgaccca
     1321 tatgcaaacc cgctttcaga caatgactgg tcgcgtctgg caaaggttaa agacctgacg
     1381 cccggcgaac tgaccgctga gtcctatgac gacagttatc tcgatgatga agatgcggac
     1441 tgggccgcga ccggacaggg gcagaaatcc gctggagata ccagcttcac gctggcgtgg
     1501 atgcccggag agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgt
     1561 gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt tccgcggctg ggtcagcagt
     1621 atcggtaagg cggtgacggc gaaggaagtg atcacccgca cggtgaaagt caccaacgtg
     1681 ggacgtccgt cgatggcaga agatcgcagc acggtaacag cggcaaccgg catgaccgtg
     1741 acgcctgcca gcacctcggt ggtgaaaggg cagagcacga cgctgaccgt ggcattccag
     1801 ccggaaggcg caaccgacaa gagcttccgt gcggtgtctg cggataaaac aaaagccacc
     1861 gtgtcggtca gtggtatgac catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt
     1921 ccggtcgtat ccggtaatgg tgagtttgct gcggttgcag aaatcaacgt caccgccagt
     1981 taatccggag aatcagcgat gttcctgaaa accgaatcat ttgaacataa cggcgtgacc
     2041 gtcacgcttt ctgaactgtc agccctgcag cgtattgagc atctcgccct gatgaaacgg
     2101 caggcagaac aggcggagtc agacagcaac cggaagttta ctgtggaaga cgccatcaga
     2161 accggcgctt ttgtggtggc gatgtccctg tggcataacc atccgaagaa gacgcagatg
     2221 ccgtccatga atgaagccgt taaacagatt gagcaggaag tgcttaccac ctggcccgcg
     2281 gaggcaattt ctcatgctga aaacgtggtg taccggctgt ccggtatgta tgagtttgtg
     2341 gtgaatgatg cccctgaaca ggcagaggac gccgggcctg cagagcctgt ttctgcggga
     2401 aagtgttcga cggtgagctg agttttgccc tgaaactggc gcgcgagatg gggcgacccg
     2461 actggcgtgc catgcttgcc gggatgtcat ccacggagta tgccgactgg caccgctttt
     2521 acagtaccca ttattttcat gatgttctgc tggatatgca cttttccggg ctgacgtaca
     2581 ccgtgctcag cctgtttttc agcgatccgg atatgcatcc gctggatttc agtctgctga
     2641 accggcgtga ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa gcggcagggc
     2701 ttgctggagg cgttcgtttt ggcccggacg ggaatgaagt tatccccgct tccccggatg
     2761 tggcggacat gacggaggat gacgtaatgc tgatgacagt atcagaaggg atcgcaggag
     2821 gagtccggta tggctgaacc ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc
     2881 agatttgacg agcagatggc cagagtcagg cgtcattttt ccggtacgga aagtgatgcg
     2941 aaaaaaacag cggcagtcgt tgaacagtcg atgagccggc aggcgctggc tgcacagaaa
     3001 gcgggaattt ccgtcgggca gtataaagcc gccatgcgta tgctgcctgc gcagttcact
     3061 gacgtggcca cgcagcttgc aggtgggcaa agtccgtggc tgatcctgct gcaacagggg
     3121 gggcaggtga aggactcctt cggcgggatg atccccatgt tccgggggct tgccggtgcg
     3181 atcaccctgc cgatggtggg ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat
     3241 gcctggtatc agggcaactc aaccctgtcc gatttcaaca aaacgctggt cctttccggc
     3301 aatcaggccg ggctgacggc agatcgtatg ctggtcctgt ccagagccgg gcaggcggca
     3361 gggctgacgt ttaaccagac cagcgagtca ctcagcgcac tggttaaggc gggggtaagc
     3421 ggtgaggctc agattgcgtc catcagccag agtgtggcgc gtttctcctc tgcatcaggc
     3481 gtggaggtgg acaaggtcgc tgaagccttc gggaagctga ccactgaccc gacgtcgggg
     3541 ctgacagcga tggcgcgcca gttccataac gtgacggcgg agcagattgc gtatgttgct
     3601 cagttgcagc gttccggcga agaagccggg gcattgcagg cggcgaacga ggccgcgacg
     3661 aaagggtttg atgaccagac ccgccgcctg aaagagaaca tgggcacgct ggaaacctgg
     3721 gcagacagga cagcacgggc attcaaatcc atgtgggatg cggtgctgga tattggtcgt
     3781 cctgataccg cgcaggagat gctgattaag gcagaggccg cgtttaagaa agcggacgat
     3841 atctggaatc tgcgcaagga tgattatttt gttaacgatg aagcgcgggc gcgttactgg
     3901 gatgatcgtg aaaaggcccg tcttgcgctt gaagccgccc gaaagaaggc tgagcagcag
     3961 agtcaacagg acaaaaatgc gcagcaacag agcgataccg aagcgtcacg gctgaaatat
     4021 accgaagagg cgcagaaggc ttacgaacgc ctgcagacgc cgctggagaa atataccgcc
     4081 cgtcaggaag aactgaataa ggcactgaaa gacgggaaaa tcctgcaggc agattacaac
     4141 acgctgatgg cggcggcgaa aaaggactat gaagcgacgc tgaaaaagcc gaaacagtcc
     4201 ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc cctgctgacg
     4261 cttcaggcag aactccggat gctggagaag catgccggag cgaatgagaa aatcagccag
     4321 cagcgccggg atttgtggaa ggcagaaagt cagttcgcgg tactggagga ggcggcacaa
     4381 cgtcgccagc tgtccgcaca ggagaaatcc ctgctggcgc ataaagacga gacgctggag
     4441 tacaaacgcc agctggctgt acttggcgac aaggtcacct atcaggagca cctgaacgcg
     4501 ctggcacagc aggcggataa attcgcacag caacaacggg caaaacgggc cgccattgat
     4561 gcaaaaaacc gggggctgac tgaccggcag gcagcgcggg aagccacgga acagcgcttg
     4621 aaggaacagt atggcgataa tcctctggcg ctgaataacg tcatgtcaga gcagaaaaag
     4681 acctgggcag ctgaagacca gcttcgcggg agctggatgg caggcctcag gtcaggctgg
     4741 agtgagtggg aagagagcgc cacggacagt atgtcgcagg ttaaaagtgc tgccacgcag
     4801 acctttgatg gtattgcaca gaatatggcg gcgatgctga ccggcagtga acagaactgg
     4861 cgcagcttca cccgttccgt gctgtccatg atgacagaaa ttctgcttaa gcaggcaatg
     4921 gtggggattg tcggaagtat aggcagcgcc attggcggcg gcgcatcagc gtcaggcggt
     4981 acagccattc aggcagctgc ggcgaaattc cattttgcgg ccggagggtt tacgggaacc
     5041 ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag
     5101 gcaaccagcc ggattggcgt aggaaatctt taccggctga tgcgcggcta tgccaccggc
     5161 ggttatgtcg gtacaccggg cagcatggcg gacagccggt cgcaggcgtc cgggacgttt
     5221 gagcagaata accatgtggt gattaacaac gacggcacga acggtcagat agggccacag
     5281 gcactgaagg cggtttatga cgtagcccgt aaggcggcaa tggatgttgt gaccgggcag
     5341 atgcgcgatg gtggtctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga
     5401 aacccggtat ggatgtggct tcggcccctt ccgtaagaaa ggtgcgcttt ggtgatggct
     5461 attcccagcg agcgcctgcc gggctgaatg ccgacctgaa aacgtacagc gtgacgcttt
     5521 ctgttccccg ttgggaggcc acggcgcttg agtcgtttct ggctgagcac gggggctgga
     5581 aggcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat
     5641 ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga
     5701 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     5761 cagcgtggtg ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg
     5821 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     5881 tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct
     5941 gacggtttct aacctgtacg gtatggtcac cgggatggcg gaagatctgc agagtctggt
     6001 cggcggaacg gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt
     6061 caacggaaac ag
//
LOCUS       DABSYT020000178         6019 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.179, whole genome shotgun sequence.
ACCESSION   DABSYT020000178 DABSYT020000000
VERSION     DABSYT020000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.179"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..149
                     /locus_tag="ID725_RS23540"
                     /old_locus_tag="pgaptmp_004708"
     CDS             <1..149
                     /locus_tag="ID725_RS23540"
                     /old_locus_tag="pgaptmp_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGV7853145.1"
                     /translation="FPNQMIEQINIALDQKGSGNFSAWVIEACRRRLTSEKRAYTSIK
                     SDEE"
     gene            complement(314..520)
                     /gene="ybcW"
                     /locus_tag="ID725_RS23545"
                     /old_locus_tag="pgaptmp_004709"
     CDS             complement(314..520)
                     /gene="ybcW"
                     /locus_tag="ID725_RS23545"
                     /old_locus_tag="pgaptmp_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HGV7853146.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            806..1216
                     /gene="ybcV"
                     /locus_tag="ID725_RS23550"
                     /old_locus_tag="pgaptmp_004710"
     CDS             806..1216
                     /gene="ybcV"
                     /locus_tag="ID725_RS23550"
                     /old_locus_tag="pgaptmp_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HGV7853147.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            1508..1801
                     /gene="bor"
                     /locus_tag="ID725_RS23555"
                     /old_locus_tag="pgaptmp_004711"
     CDS             1508..1801
                     /gene="bor"
                     /locus_tag="ID725_RS23555"
                     /old_locus_tag="pgaptmp_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGV7853148.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(1892..2074)
                     /gene="rzoD"
                     /locus_tag="ID725_RS23560"
                     /old_locus_tag="pgaptmp_004712"
     CDS             complement(1892..2074)
                     /gene="rzoD"
                     /locus_tag="ID725_RS23560"
                     /old_locus_tag="pgaptmp_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGV7853149.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2291..2767)
                     /locus_tag="ID725_RS23565"
                     /old_locus_tag="pgaptmp_004713"
     CDS             complement(2291..2767)
                     /locus_tag="ID725_RS23565"
                     /old_locus_tag="pgaptmp_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGV7853150.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(2754..3059)
                     /locus_tag="ID725_RS23570"
                     /old_locus_tag="pgaptmp_004714"
     CDS             complement(2754..3059)
                     /locus_tag="ID725_RS23570"
                     /old_locus_tag="pgaptmp_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGV7853151.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(3381..4070)
                     /locus_tag="ID725_RS23575"
                     /old_locus_tag="pgaptmp_004715"
     CDS             complement(3381..4070)
                     /locus_tag="ID725_RS23575"
                     /old_locus_tag="pgaptmp_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGV7853152.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(4067..4207)
                     /locus_tag="ID725_RS23580"
                     /old_locus_tag="pgaptmp_004716"
     CDS             complement(4067..4207)
                     /locus_tag="ID725_RS23580"
                     /old_locus_tag="pgaptmp_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGV7853153.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(4204..4566)
                     /gene="rusA"
                     /locus_tag="ID725_RS23585"
                     /old_locus_tag="pgaptmp_004717"
     CDS             complement(4204..4566)
                     /gene="rusA"
                     /locus_tag="ID725_RS23585"
                     /old_locus_tag="pgaptmp_004717"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGV7853154.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
     gene            complement(4563..4853)
                     /locus_tag="ID725_RS23590"
                     /old_locus_tag="pgaptmp_004718"
     CDS             complement(4563..4853)
                     /locus_tag="ID725_RS23590"
                     /old_locus_tag="pgaptmp_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGV7853155.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(4846..5016)
                     /locus_tag="ID725_RS23595"
                     /old_locus_tag="pgaptmp_004719"
     CDS             complement(4846..5016)
                     /locus_tag="ID725_RS23595"
                     /old_locus_tag="pgaptmp_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGV7853156.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(5016..5471)
                     /locus_tag="ID725_RS23600"
                     /old_locus_tag="pgaptmp_004720"
     CDS             complement(5016..5471)
                     /locus_tag="ID725_RS23600"
                     /old_locus_tag="pgaptmp_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HGV7853157.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(5468..5566)
                     /locus_tag="ID725_RS23605"
                     /old_locus_tag="pgaptmp_004721"
     CDS             complement(5468..5566)
                     /locus_tag="ID725_RS23605"
                     /old_locus_tag="pgaptmp_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853158.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
BASE COUNT         1604 a         1446 c         1320 g         1649 t
ORIGIN      
        1 gctttcctaa ccaaatgatt gaacaaatta acatcgctct tgatcaaaaa gggtccggaa
       61 atttctcagc ctgggtcatt gaagcctgcc gtcggaggct aacgtcagaa aagagagcat
      121 atacatcaat taaaagtgat gaagaatgaa catcccgcgt tcttccctcc gaacaggacg
      181 atattgtaaa ttcacttaat tacgagggca ttgcagtaat tgagttgcag ttttaccact
      241 ttcctgacag tgacagactg cgtgttggct ctgtcacagg ttaagtagtt tgaatgatta
      301 gcagttatgg tgatcagtca accaccaggg aataatcctt catattatta tcgtgcttca
      361 ccaacgctgc ctcaattgcc ctgaatgctt ccagagacac cttatgttct atacatgcaa
      421 ttacaacatc agggtaactc atagaaatgg tgctattaag catatttttt acacgaatca
      481 gatccacgga gggatcatca gcagattgtt ctttattcat tttgtcgctc catgcgcttg
      541 ctcttcatct agcggttaaa atattacttc aaatctttct gtatgaagat ttgagcacgt
      601 tggccttaca tacatctgtc ggttgtattt ccctccagaa tgccagcagg accgcacttt
      661 gttacgcaac caatactatt aattgaaaac attcctaata tttgacataa atcatcaaca
      721 aaacacaaag aggtcagacc agattgaaac gataaaaacg ataatgcaaa ctacgcgccc
      781 tcgtatcaca tggaaggttt taccaatggc tcaggttgcc atttttaaag aaatattcga
      841 tcaagtgcga aaagatttaa actgtgaatt gttttattct gaactaaaac gtcacaatgt
      901 ctcacattat atttactatc tagccacaga taatattcac attgtgttag aaaacgataa
      961 cactgtgtta ataaaaggac ttaaaaaggt tgtgaatgtt aaattctcaa gaaatacgca
     1021 tcttatagaa acgtcctatg ataggttgaa atcaagagaa atcacatttc agcaatacag
     1081 ggaaaatctt gctaaagcag gagttttccg atgggttaca aatatccacg aacataaaag
     1141 atattactat gcctttgata attcattact atttactgag agcattcaga acactacaca
     1201 aatctttcca cgctaaatca tcacgtccgg tttcttccgt gtcagcaccg gggcgttggc
     1261 ataatgcaat acgtgtacgc gctaaaccct gtgtgcatcg ttattaatta ttcccggaca
     1321 ctcccgcaga ggagtttccc tgtcagggct gcggacatag ttaatccggg aatacagtga
     1381 cgatccttcg catctgacat acattaataa atattaacaa tatgagattt caactcattg
     1441 tttagggttt gtttaatttt ctacacatac gattctgcga actttaaaaa gcatcgggaa
     1501 taacaccatg aaaaaaatgc tactcgctac tgcgctggcc ctgcttatta caggatgtgc
     1561 ccaacagacg tttactgttc aaaacaaacc ggcagcagta acaccaaagg aaaccatcac
     1621 ccaccatttc ttcgtctctg gaattgggca gaagaaaact gtcgatgcag ctaaaatttg
     1681 tggcggcgca gaaaatgttg ttaaaacaga aacccagcaa acattcgtaa atggattgct
     1741 cggttttatt actttaggca tttatactcc gctggaagcg cgtgtgtatt gctcacaata
     1801 attgcatgag ctgcccatcg atatgggcag ctctatctgc actgctcatt aatatacttc
     1861 tgggttcctt ccagttgttt ttgcatagtg atcagcctct ctctgagggt gaaataatcc
     1921 cgttcagcgg tgtctgccag tcggggggag gctgcattat ccacgccgga ggcggtggtg
     1981 gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca
     2041 tcatcatgca gagcatcatt ttcagctttc gcatcagcta actccttcgt gtattttgca
     2101 tcgagcgcag caacatcacg ctgacgcatc tgcatgtcag taattgccac gttcgccagc
     2161 ttcagttctc tggcattttt gtcgcgctgg gctttgtagg taatggcgtt atcacggtaa
     2221 tgattaacag cccatgacag gcagacgatg atgcagatga gcagagcgga gataatcgcg
     2281 gttattctgc tcatacctca ctctctctga ccgttccgcc agcttctttg aattttgcaa
     2341 tcaggctgtc agccttatgc tcgaactgac cataaccagc gcccggcagt gaagcccaga
     2401 tattactgca acggtcgatt gcctgacgga tatcaccgcg atcaatcata ggtaaagcac
     2461 cacgctcttt aatctgctgc aatgccactg cgtcctggct tttgggggag aagtctttca
     2521 aaccaagctg tttacggtaa gcatcccacc agcgtgaaag aagctgataa cgtccggcgg
     2581 ctgttgattt gagtttgggg tgtagcgtga caagtttgcg agggtgatcg gagtaatcag
     2641 taaacagttc gccgccaaca ataacatcat aaccgtggtt acgtgtcggt tgtcgcccgt
     2701 tatccgttcc ttctgaccac gccaacatat cgaggaaggc tttacgctga ggattaagat
     2761 tttgcatttt tcacccctgt cagtcgttcc cagaagtacg tcagtgcaac cgaacccatc
     2821 gcaccactaa tccccgctgt cgcgagaatc atgtaaatac tgaatccact ttcgatgctg
     2881 atcaggccac caataacacc ggtgaatcct gataccacta tttgagccag agcatttatc
     2941 caactccacg ttgctttact ctgcttcaca tctatcaggt agcggaccag accgccccaa
     3001 cctgcgatga tcagcaaaac gagccagaac gctccggcaa ggctctcttt ttcgtgcata
     3061 tgaatagcca atgtttcgcc gccgacaaaa ggccgggacg ttaaatgtca gaaatcaggc
     3121 tcacggggta atttaacgac aaagcacgga gttgatgctc cccacaagcc tggaataaaa
     3181 aagccagcat gtagctggca acagagggct aagcaatatc aactcaacag ctgaagaccc
     3241 cctggctggg gtaggttgga aggctactca ccgttcagaa acagaaaagc ccaaggcttt
     3301 aaacctcggg cttgaatttg gattactgcc agtgcgtaca acattggcaa aatatcagat
     3361 ttacataaaa tatatgattt ttaatccagt tttgcaatat cttgctgtga aaatgtggtc
     3421 ttttgttttg aacgtgtttt cgttaaaagc aataaagctt ggctatcaag ctgtagaaaa
     3481 atgtgtttca ttgcaaccca gcgttcagta aatgtctcag accagttttt tgatgtcact
     3541 cccaccagtg atgccagctc ctggtattca taggtcttac gccctgccag ctcgttcttc
     3601 acatcctgtg ccgccagcca gatcaacttc ttcaaacgtt ccagtgtctt acctgcaatt
     3661 ttcctggtac ccaacagagt cttaaactcg ctccatgccc actgcgttat ggtgacctga
     3721 tgttcccagc gaacactttc gctgtaactc cacagcaacc acgctttctg atgttcttcg
     3781 agagacagaa ccgcgcggcg ccatgacgag gttgagaact caacctggct gaccagtgca
     3841 atggatgaac cttttgcgta cgactgctta ccggaaatcg gcggattatc cagcgtaatc
     3901 atcctgccag ttacctcatc cagaatgcgc ggcttctttc gtttgtatgt accagtatca
     3961 aattgtgcat gctccagcca ggcttcaagc tgtcctttcg ttgctccgct caaatcagcg
     4021 gtagccacaa tgagttgctc gcggacatac tgtaaatatt gggtattcat gcggcagctc
     4081 ctttcagtgt tttggcgtaa ttcttcagta tccggtaatc ggtcaaaaca gaaccaggaa
     4141 aacgatataa gcgcaggcgc atccagcggt ggcgaagacg ttctgccata taagactcaa
     4201 acatcattca ttccccagtt cggtgatggt cagctccagc ttcccacctt tggtaacggg
     4261 catcttcaca acacggtaat caacgacctg agcatcatcc agccagaaac ctgctttggt
     4321 gagtgcgtca aaagcggctt tttgcagatt atccaggtca cggcgacggc gatccggcat
     4381 gtggcactca atacggattt tcactggcat agccaggccg atatccagca tggagccttt
     4441 gatgattcgg gcgacgttat cacggtatgc ctgcccttct gcgctgatgt gcgtgcgccc
     4501 gcgattatgc cggtagtagc gattattgct cggaggccag ggtaatgtga tgctgtaggt
     4561 attcacgcct taataacccc ctctttcagc cagataacct gtgttctcgc cataccttcc
     4621 agcgcgcatt cttttgcata tgcagcatcg acaaaatgtg tgcggcggtc gatttcgtcg
     4681 tggcaggcag aacatgcaat ggtggcaatc aagtctggcg gtttggtacc ggtgccgcac
     4741 aatccagtca gccggatatg tgccagtaca gacgtttcag ggttgccatt acatacgcca
     4801 gggattctta cttggcattc ccgactacgc gctgcttttc tcaaatcagc catgattcct
     4861 ccttgctgcc agtcgcaacc attttttatc aaccaggctg gcggtatatc cgagcagtgt
     4921 tggtatttcg gatggcttca gctcaggttt acgcttacga cgatttggta ctttgtagat
     4981 gtgtccgttc atgacacgaa taagcggtgt agccattacg cctcctgctt gtcacgcagc
     5041 agctggaact cgcagctctg tggaatagtc aggtggcagc caatattcat cgcccaggct
     5101 tcaaccttac acaggaagac atacatctct ccggtaccaa gatcggaggt atggcgtaac
     5161 gactggatag tagtgatttc gccggttacg acatcaacca ggtccttggt ttcataaccg
     5221 aggtatgtgt gtttgagagc atcttttacc catgctgaag tagcgaacga tttccccctg
     5281 ctgatgaggt attcactgat ttcgctgtac cacatgtggc tgagtgcatt ctgggaaaga
     5341 ctgcgtctct cgcgccacgg tttaagcacc atgcgaaagc atttgccgtc ttccagataa
     5401 gtctggatct gctgaccgac agcggtgaag ttaccgcgat gtaatttgat gccatcttgt
     5461 gggaggttca cgcttcacct ccgcagagat caaacgctgg atgcaagaca tcgcaggtgc
     5521 atttctgcat ctgtgacaag gtaaggagtt cagattgtgg tcgcatttaa gtccccttaa
     5581 atgcgcagaa gtcaccggag ttgttcaagc tccggtgaca ttattatgga aggttgatac
     5641 taaaaaatca aaaattgaat atcattggcg atagtctagc ttgtgatatc cacaagctac
     5701 ttcaatgaag cgacgagtaa cattttgtgt catccatgtt ttttcagtta agtgccacac
     5761 ccagttcaaa atttgctgat gtgttgaaca tgtactaagc gggatgttgt attcataagc
     5821 aacattgatt ccgcaaaatc ggtgtcgtgt catgaccgat tcacgttgac ctgcgccctg
     5881 ttgagtgcct ggggatattc ttgctcgata gttaatatca tagtagttac tttactttca
     5941 taccatagca cggttgaaaa agtgattatt actcaaaaat aaacctcacc atcaaccata
     6001 tatttgagag cacttatcg
//
LOCUS       DABSYT020000179         5901 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.180, whole genome shotgun sequence.
ACCESSION   DABSYT020000179 DABSYT020000000
VERSION     DABSYT020000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.180"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            121..818
                     /locus_tag="ID725_RS23610"
                     /old_locus_tag="pgaptmp_004722"
     CDS             join(121..370,370..818)
                     /locus_tag="ID725_RS23610"
                     /old_locus_tag="pgaptmp_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HGV7853159.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            835..1095
                     /locus_tag="ID725_RS23615"
                     /old_locus_tag="pgaptmp_004723"
                     /pseudo
     CDS             835..1095
                     /locus_tag="ID725_RS23615"
                     /old_locus_tag="pgaptmp_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(1237..2361)
                     /locus_tag="ID725_RS23620"
                     /old_locus_tag="pgaptmp_004724"
     CDS             complement(1237..2361)
                     /locus_tag="ID725_RS23620"
                     /old_locus_tag="pgaptmp_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HGV7853160.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(3091..3288)
                     /locus_tag="ID725_RS23625"
                     /old_locus_tag="pgaptmp_004725"
     CDS             complement(3091..3288)
                     /locus_tag="ID725_RS23625"
                     /old_locus_tag="pgaptmp_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7853161.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(3354..3624)
                     /locus_tag="ID725_RS23630"
                     /old_locus_tag="pgaptmp_004726"
                     /pseudo
     CDS             complement(3354..3624)
                     /locus_tag="ID725_RS23630"
                     /old_locus_tag="pgaptmp_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3853..4125)
                     /locus_tag="ID725_RS23635"
                     /old_locus_tag="pgaptmp_004727"
     CDS             complement(3853..4125)
                     /locus_tag="ID725_RS23635"
                     /old_locus_tag="pgaptmp_004727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853162.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWEAIYRSTSHYGKNVLLLQLA"
     gene            4445..4639
                     /locus_tag="ID725_RS23640"
                     /old_locus_tag="pgaptmp_004728"
     CDS             4445..4639
                     /locus_tag="ID725_RS23640"
                     /old_locus_tag="pgaptmp_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853163.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            4982..5110
                     /locus_tag="ID725_RS23645"
                     /old_locus_tag="pgaptmp_004729"
     CDS             4982..5110
                     /locus_tag="ID725_RS23645"
                     /old_locus_tag="pgaptmp_004729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853164.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(5420..5755)
                     /locus_tag="ID725_RS23650"
                     /old_locus_tag="pgaptmp_004730"
     CDS             complement(5420..5755)
                     /locus_tag="ID725_RS23650"
                     /old_locus_tag="pgaptmp_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853165.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1723 a         1346 c         1257 g         1575 t
ORIGIN      
        1 aaaaatgcaa ctataaaaaa cagtcatcag tcggctgctt ttcaatgttt accttatcat
       61 tttctggtaa tgctgccaac ttactgattt agtgtatgat ggtgatttta aggtgcttgc
      121 gtggcttcca tttccatcag atgtccttcc tgctccgcta ctgaaggcgt ggtgcgtaac
      181 ggcaaaagca ctgccggaca tcagcgctat ctctgctctc cttgccgtaa aacatggcaa
      241 ctacagttca cttacaccgc ctctcagccc ggtaagcacc agaaaatcat tgatatggcc
      301 atgaatggcg tcggatgtcg cgccagtgca cgcattatgg gcgttggcct caacacggtt
      361 ttacgtcact taaaaaactc aggccgcagt cggtaacctc gcgcatacaa ccgggcagtg
      421 atgtgattgt ctgcgctgaa atggacgaac agtggggcta cgtcggtgct aaatcacgtc
      481 agcgctggct gttttacgcg tatgacagga tacggaggac ggttgtggcg cacgtcttcg
      541 gtgaacgcac tctggccaca ctggagcgtc ttctgagcct gctgtcggcc tttgaggtcg
      601 tggtatggat gacggatggc tggccgctgt atgaatcacg cctgaaggga aagctgcacg
      661 ttatcagcaa gcgttacact cagcgcattg agcgacataa tctgaatctg agacaacatc
      721 tggcaaggct gggacggaag tcactgtcgt tctcaaaatc ggtggagctg catgacaagg
      781 tcatcgggca ttatctgaac ataaaacact atcagtaagt tggagtcatt acctgagaat
      841 aatacctcct gggtaagtga gcagatactt tcagcgcctc cgaaagatgt aaatattcga
      901 cttggagtcg gttctttaga aggtacaacc gtctcacatg ttcagcgatt gcatcattcg
      961 ttaatcgcag ctggtttgga aagtaacctc actgtctata ccggtggtca cgattatgcc
     1021 tggtggcgcg gagcaattat tgatgaatta gcaaattata attgcaggaa ggtatcaaat
     1081 aataactctg tgtaaaatat cgttttatcg tcagataact ctttctgcaa tattattatg
     1141 ggagactatc tcatttattt agtgatgtcc ttcatttatg tgatgaagga cattatatta
     1201 ctcataacat ttctcgttga aaatttcagc aatacgctac aattgtttcc cgttgttttt
     1261 taattttctc atcaaaacct ctgcaatctc tgcaggactg ggttgttcag ccatttcagc
     1321 ggctacctga tctgaacaga agcgtagtga atcatcataa agaaggcgat ttaccaaatc
     1381 actggtcagt ccatgcttgt ccggaataat cccacaccca cgcttcgcaa caatttctgc
     1441 attaacagag cgatctgcac cttcgccaaa cacaatctgt ggtattccac tatacagcgc
     1501 tgtcagggta ttacctgcgc caccgtgatg aataaatcca tcagctccat taaggaatac
     1561 acccataggt atccagtcaa ccagacgtac atttgatggt agttttcgta aaccagtacg
     1621 agcatttatt gcaagttgca aaatgatatc agcatcaact tcatttgcag aatccataac
     1681 ccatgaaatc agctccagac catcaaccat tggttttaca gtgccaaggc tgataagtaa
     1741 acgtttccga tcagaatccc tgtcccacca tgtttccttt acagcacctc cgttataagg
     1801 aatatatctc attgagataa ccggttctcc agcattttta agaatgctca tacttggggg
     1861 agcaacatca atccatgcta aatcacatag tggaccgctg actccatgcc gcctgtaagc
     1921 attgcttaaa gaacggttta acatctgaat atgggcagat gtatgcgcga atccaacagc
     1981 cagcatcact gaaggaattc tatatttagc agcaaccaat gggcctgccg gatcaagagg
     2041 gggatagact attaaatctg gcctccattt tcctgcaaaa tcgatgaggt tatctgccat
     2101 ttcatcgcta aaaaaagaga aatgaccaat aatattactt tttttcctca actcttcctg
     2161 atggcgatat ccagcctctg aatctaaacc tggaactgca tcaaaaacaa ccagtccagc
     2221 ttctgctgct ttattcgcga atttgccagc gctactaatt actacatcat gtccaattac
     2281 acgaaaggcc tgagccagag aaatcaatgg gtataacaaa ccgtacagcg gtgggccaat
     2341 aaaaagaata cgttttcgca ttaaaaaagt ccgtcttctg cttacctgtt tatttttcta
     2401 tctctaagaa aacataatta atattatttt tatgtgagct tatgcacaaa aataaccaag
     2461 tatattatct tctggtagag attcaggata cagtttgtag gagctttctt ttttgaacaa
     2521 tgatttgcag gacagttatg ctaaactagc ggaatcagta tcacaacaat aaatgactga
     2581 tcatacctga aatttgaatt aacaggcatc atgaatgtca atagtctaag cggtgactac
     2641 ttatatcgct tgcataaata ctaaacactg actagaaaag tattggataa ccccgtcact
     2701 aaaagtaacg cttcggttat ttgctcggaa tgtattaacg ccaagattgt tagccgttac
     2761 gccttatatc actggcgctg acaaatgttt cttttctaat ttactgaacc ggcgcagaca
     2821 cgcaggaaag cacgccggca ccggtgtaat gccagttcta atcaataaga atggaatgac
     2881 gaatcagtca ggaagggata acaacccgct gctgaacgga aggtatcagc aggaagatga
     2941 acgggaatat cgccagcacc aagggcaggg ccgggttcca caataccagc gctggcattt
     3001 tgcacattgc cagcgtaccg gcattacaac acgtttcatg ctgctgcccc ccaggattcc
     3061 tcgattcgtt gctacatcca ctgctctact tcacttccac tgctccactt cacttttgta
     3121 ccagcggctg ctgcgcccca gtttgatggg tttagggaaa tggccttcac tgatcaactt
     3181 gtaaaaccat ttgtcggtca taccggtgaa actggtgata tagttcatgt cgatcaggga
     3241 attatcggca cgcagaaata cgctgtcgtg gcttgttgta gtcaacatgg tcagaatgtc
     3301 ctgtgagatt tatgaagatt gtgcgaatgc gggaaatcta ctgagctgtg ctttcagaac
     3361 tggcctgtta cgggatacca gggattaccg gcggggtaac gggcttccgg atcatacaca
     3421 ccacgattat cgcggacaaa atcactgaac gcccatatca cctctttaag tatgtcttcg
     3481 cagtccggta aatgacgatc cagcgccaca tcccgggtgg tacagctttg atgcgcccgg
     3541 tgacacaaag cccggattgt tccagacatc ctgaatcaaa cgccccagat taggggcgtc
     3601 gaaatatgcc tctctgacca ttatattccg gcgtataggt agcaggtctg aagtgacaat
     3661 gcgtcacctg acgttaaaag tcacccaaga tgaccccagt gggggcaaca agtataaaac
     3721 cgttttttga ttaagtggtc ggatatgaaa aggggaaggt ttatgtgaaa aatacctgtg
     3781 ttttctttga aaacacacaa cagtatcagg ttatttatgt ttatacagtg cgttccaaac
     3841 tgacctgaat aattacgcaa gttgcaacaa taaaacattt ttgccgtagt gtgaggtact
     3901 ccggtaaatt gcttcccagg tgcagacaat gactaacgaa gaactacagg caatcgcatt
     3961 atccatgctt gagcgccagc gcgaaaaagt acaccagcaa cgagagtctt tgcttaaccc
     4021 atctccattc accaggccaa aacgattgga gcttgaagca ttcttggaca aatacccacg
     4081 gcggcttaaa tcaggcaaaa ccagaccacc aggctgatag ctcacagttt gttctccgat
     4141 tcaataaaac ggataagaac catcagcgcg gtagcgtaag gcactacagc gcattgcttt
     4201 acgagtaata acaatggatt tatcaccaga agacgcaaag tttttacgag aactgcaaca
     4261 ccgtatgctc agagaagcca ttatcgaaca gcaaacgggc atgagcgcca tagacccatt
     4321 gcgatacaaa cgggcctcat ctcctttagt tgacgacaat tccaatgcgg ataaaacacg
     4381 tccaccacac tgattacatt tttcattagc acagtggtgt gatgcagcat acgaggaaaa
     4441 ccggatgaat gagaacaaaa taaaaaggct ggagcaactg ttacaggcac gccaacggga
     4501 gtttgccact aaatggggac gtgccgcgct gccacatgaa cgcctaacct attttccact
     4561 tgaagacctc attaaaaata atggcaaaaa aatcaggtct gataagaaac acttcttaga
     4621 atgcaatgaa gacaaataac aacaatctgc atattgccac caaccacaga tgtggcatcg
     4681 ttatcagcgc acttgatttt ctttatatcc actgacgagt ttgccggtca ttctggtatc
     4741 atgcagatat ttacggcgta caagcacact agcatgacat tgtaataagg cctgagccta
     4801 acgctttaat gtgtgtcata cactcagtgg cgtaatcgtt gacaatatgg tgcatgtaat
     4861 cgtaagcgtc cagcgaaatc catctggtca taatctgaag catccgacaa agtggtgtcc
     4921 accaaataag tgatctgcgc ccaatatcct gagccggaac tttgacaaag gccaacgaaa
     4981 aatgggagcg gatgttacag agttctcggt acaggggaaa agtggtacct gtcgccagtt
     5041 ctccgatctg tttaaccggg aaattatctc ctacagcttg tcagaaaggt cggtgatgga
     5101 gcatcgttaa tacgatgcta tacgatgcat tcacagtgcc cggccagagg atgccccgtc
     5161 gctgcatatg gatcagggtt ggcaatatcg aattgcaggc tataggcaaa gttaaagccc
     5221 catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatgaag
     5281 aacttcttca gcacactgct aaaacgcata gtgatcgagc gctgattctg gcgaacaact
     5341 gaactaatac atcagaatct gcattatgtt aaataaatat aaaaagatgg tttaaatacc
     5401 ccgttacttt tgacttacac tatacggtat cgcatcgttt aatattcgca ccggccagat
     5461 ttttatttct attagttgtc acaatactga atgcgtacga ccacagtatt ctggctcctg
     5521 tgtggttatg ctttaattct gcgttccggg cagataagca gttgcttgca ggaatccttc
     5581 ttgtgttaat gtcagttccc cttttaccag tgctgatttc cacattccgt ccaacagagc
     5641 ttatagcctt tccctggatt atagcattgt ccggctgaag ttctttttga ataataatag
     5701 aagcactgct ggcagatcca gtccgttttt cataacccac tgtactgata accataatct
     5761 aatcagtaga aattgagtcg aaaataagca ctactccata caggataatt agaggtcagt
     5821 ttgattattc acaattcatc atcagcattt tctatttctg acgaaatcaa tatgaaaata
     5881 accatatatg ataattatta t
//
LOCUS       DABSYT020000180         5891 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.181, whole genome shotgun sequence.
ACCESSION   DABSYT020000180 DABSYT020000000
VERSION     DABSYT020000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.181"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1671)
                     /gene="rhsC"
                     /locus_tag="ID725_RS23655"
                     /old_locus_tag="pgaptmp_004731"
     CDS             complement(<1..1671)
                     /gene="rhsC"
                     /locus_tag="ID725_RS23655"
                     /old_locus_tag="pgaptmp_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HGV7853166.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDITRY
                     RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRF"
     gene            1900..2508
                     /locus_tag="ID725_RS23660"
                     /old_locus_tag="pgaptmp_004732"
     CDS             1900..2508
                     /locus_tag="ID725_RS23660"
                     /old_locus_tag="pgaptmp_004732"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGV7853167.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            2606..3997
                     /gene="selA"
                     /locus_tag="ID725_RS23665"
                     /old_locus_tag="pgaptmp_004733"
     CDS             2606..3997
                     /gene="selA"
                     /locus_tag="ID725_RS23665"
                     /old_locus_tag="pgaptmp_004733"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGV7853168.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3994..5838
                     /gene="selB"
                     /locus_tag="ID725_RS23670"
                     /old_locus_tag="pgaptmp_004734"
     CDS             3994..5838
                     /gene="selB"
                     /locus_tag="ID725_RS23670"
                     /old_locus_tag="pgaptmp_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGV7853169.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1255 a         1611 c         1716 g         1309 t
ORIGIN      
        1 gaagcggtca tggtcataac gggttacata accggaacag tcggtgaagc tcagcaactg
       61 accgtaacgg ctccacgtca tggttttccg gctgccggtg gcatcttccg ttgcgcaggg
      121 taagtcactg tgtggattat cataacggta gcgggtgata tcgccgtcag gggcagtttc
      181 ctgaatcaga cggccccatt catcatattc ccggcgtatt tccagcccgt caggcccggt
      241 ggctgacgtt aactggctgt ggtggttata gtaaaacgcc gatgccctgc catccggcgt
      301 ggtgatgcgc gtgatgaggc ccgtcaccac atccgggctg tattctgttg tcctgcctgc
      361 ggcatccgtc tgtgccctga gcctgcccac cgcgtcaaac tgactctgcg tgacgctgcc
      421 gtccgcgtgt tcctttttca ccacccgctt cagcccgcct tcaccctgcg tgtgcaggac
      481 ttcacggcgg ttcaggctgt cggtgatggt gatgcggtct ttctcatact gatacgtgta
      541 gcttaagcct gccgggttta gctgttctgt cacccgcccg tcgctgtcgt aacggtaacg
      601 gatttccggc cggcccgtgt gacggtgcgc caccatccgg ccccggtatt tatcatcgta
      661 agtaaagctg cgcacctgtt tgccactacg gtcatacacc accgccagtt cgccgcgcgg
      721 cgtccagcca tagcgcacca gcggcgcggc aggtaaattc tccgggtatt ccgggtcgtg
      781 cgtcagccac acggcagaca gacggatgcc gttgtcccgg ccatattcgg tgtaacccgg
      841 cagggtatca ggaaaagcgg acggttccgt cccgccggaa atggcctgct gccgggcttc
      901 ttctgcccgc tgcgcctgcg tggtcagtac cagccggaag tgacgcccgg caccatccgt
      961 cacgccggtg atttcgccgc tgaattcacc ggcggcttcg cggtggaacg tctgtgtgcg
     1021 cccgaagcgg tccaccagcc cggtcagtac ccggtacggc ggcagcggcg caggcagcac
     1081 ctcatccgct tccggcaccc gctcacacca gccgagcagc caccacggcc cctgcggact
     1141 gtttgtcgcc agataacgat gcggacttaa gcggagttct tccggcagcg cctgccagag
     1201 tgcggccagc cggtgacctt catccagttt cgccacgccg ccgcgcacca gccagagtga
     1261 ctcgctgcgg ctgtaaccgt cctcaccggg aaacaggtgc tcaaaataca ggcttctgcc
     1321 gccgttatca ctgagtatca gtgtgttatc gcgcagctgt aagcggatat ccgcaggcat
     1381 tttccagccg gggccgaggc tccccaccgg cgcgggcgtt tttgtccggt aactgctgta
     1441 ggtgcgggag aggatgaacg gcagcgggcc gggcagggcg atgtcggttt caccgggaag
     1501 gacctttgca ccgagcaggg gattgaccgg atggccggac gtcactccgc cggggcacac
     1561 cgaacaggcc acgccggtgg gggcaccaat gcgcaccccg gctgaaccct gaacaatgct
     1621 accgccatac tgcgtcatgt cgccctgacg cgccgccggt tttccgctca tctgctcatc
     1681 cttgttttat cgggttattt gtctgcttcc aggcaaaacc ctacagattt ataagaaagc
     1741 cttaagtgag atatccatca gatagaaaca attaggaaaa tatttatatc accatgataa
     1801 atatgtgatt tatttggaag taacaaacca tcaacatttt ctgccctttt tcccatcacc
     1861 aggccatact gatatttttc ctcgcacaaa ggactcggca tgaaactcgt cggtagctac
     1921 accagcccgt ttgtacgcaa actttctatt ctgttgttag aaaagggcat aactttcgaa
     1981 tttattaatg aactgcccta taacgcggac aacggcgtga cgcaatttaa cccgttagga
     2041 aaagtgccgg tgctggtgac cgaagagggc gaatgctggt ttgattcgcc gatcatcgct
     2101 gaatatattg aattaatgaa tgtcgctccg gcgatgttgc cgcgcgatcc gctggagtcg
     2161 ttgcgggtgc gcaaaattga ggcactggcg gatggcatta tggatgccgg gctggtatcg
     2221 gtgcgtgaac aggcgcgtcc agcggcgcag cagtctgaag atgaattgtt acgccagcgg
     2281 gagaaaatca accgcagtct ggatgtgctg gaaggatatc tggtcgatgg cacactcaaa
     2341 accgatacgg tcaatctggc gactatcgcc attgcctgtg ccgtcggata tctcaatttc
     2401 cgccgcgttg cgccaggttg gtgtgtcgac cgcccacact tagtcaaact ggtcgaaaac
     2461 ctgtttagcc gcgaaagttt tgctcgcacc gaaccgccaa aggcttgatg cgcgatatgt
     2521 cctcctgacc catctcacgt tacaatccgt ggttatgtta aacgcccttc tccgtgtgag
     2581 agggccttga tcagccaggt ttcctatgac aaccgaaacg cgttccctct atagtcaact
     2641 tccggctatt gatcgcttat tgcgcgatag ctccttcctt tctttgcgtg atacttatgg
     2701 tcacacccgc gtggtggaat tgttgcgtca gatgctcgac gaagcgcgag aagtgattcg
     2761 tggcagccag acgctgcctg cgtggtgtga aaactgggcg caagaagtcg atgcccggtt
     2821 gacgaaagaa gcgcagagcg cgctgcgtcc ggtgatcaac ctgacgggaa ccgtgctgca
     2881 taccaacctt gggcgagctt tacaggcgga agccgcggtg gaagccgttg cgcaggctat
     2941 gcgttcgcca gtgaccctcg agtacgatct ggacgacgcc ggacgcggac atcgcgatcg
     3001 ggcgctggcg cagctgctgt gccgtattac gggggcggaa gatgcctgta tcgtcaataa
     3061 caatgcggcg gcggtgttat tgatgttggc ggccactgcc agcggaaaag aggtggtggt
     3121 atctcgcggc gaactggtgg agattggcgg cgcgtttcgt attcccgatg ttatgcgtca
     3181 ggcaggctgc accctacacg aagtagggac caccaaccgc acgcacgcga atgattatcg
     3241 tcaggcggtg aatgaaaata ccgcactgtt gatgaaagta cataccagta actacagcat
     3301 tcaggggttc accaaagcga tagatgaagc ggaactggtg gcgctcggca aagagctgga
     3361 tgttcccgta gtgactgatt taggcagtgg ctcgctggtc gatcttagcc agtacggttt
     3421 gccgaaagag ccaatgccgc aggagttgat tgcggcgggc gtcagtctgg tgagcttctc
     3481 cggcgacaag ttgttaggcg ggccgcaggc agggattatc gttggtaaaa aagagatgat
     3541 cgcccgcctg caaagccacc cgctgaagcg tgcattacgc gcggataaaa tgaccctcgc
     3601 ggcgctggaa gccacgttgc gtctttattt acaccctgaa gctctgagtg aaaaattacc
     3661 gaccctgcgc ctgcttaccc gcagcgcaga ggtcattcaa atccaggcac aacgtttaca
     3721 ggctcccctt gccgcacatt acggcgcgga gtttgcggta caggttatgc catgtctttc
     3781 gcagattggc agtggttcgc tgccggttga tcgcctgccg agcgcggcat taacgtttac
     3841 accccatgat ggacgcggta gccaccttga gtcattagcc gcccgctggc gtgaattgcc
     3901 agtgccggtg attggtcgta tttatgacgg acgattgtgg ctggatttac gctgccttga
     3961 agatgagcaa cggtttttgg agatgttgtt gaaatgatta ttgcgactgc cggacacgtt
     4021 gaccacggca aaacaacctt attgcaggcg attactggcg taaatgctga ccgtctgccg
     4081 gaagaaaaaa agcgcggcat gaccatcgat ctcggctatg cctactggcc gcagccggat
     4141 ggtcgcgtgc ctggttttat cgacgtaccc ggtcatgaaa agtttctttc caacatgctg
     4201 gcgggcgttg gtggtatcga tcacgcgctg ttggtggtgg cgtgcgatga cggcgtgatg
     4261 gcacagaccc gtgagcatct ggcgattttg cagctgaccg gtaacccgat gctgacagtg
     4321 gcgctgacca aagccgatcg cgtagacgaa gcgcgtgttg atgaggttga acgccaggta
     4381 aaggaggttc tgcgggaata cggttttgct gaggcaaaac tgtttatcac cgcagcaacc
     4441 gaaggtcggg gaattgatgc cctgcgcgag catctgcttc agttgccgga acgcgagcac
     4501 gccagccaac atagtttccg cctcgcgatt gaccgcgcat ttaccgtaaa aggtgccggg
     4561 ctggtcgtca ccggtacggc gttaagcggg gaagtgaagg taggcgattc actctggctg
     4621 actggtgtaa ataaaccgat gcgtgtacgt gcgctgcatg cgcaaaacca gccaacagaa
     4681 accgccaatg ccgggcagcg tatcgcgctt aacatcgcgg gtgatgcgga aaaagagcag
     4741 attaaccgtg gcgactggct gcttgccgat gtgccgccag agccgttcac acgggtgatt
     4801 gtcgagcttc aaacccatac accgctgacc cagtggcagc cgctgcatat tcaccacgcc
     4861 gccagccacg tcacgggacg cgtttcactg ctggaagata accttgctga actggtcttc
     4921 gaccccccgt tatggctggc agataacgac cgcctggtat tgcgcgatat ctctgcccgc
     4981 aacacgctgg ccggagcgcg cgtcgtgatg cttaacccgc cgcgtcgcgg taaacgtaaa
     5041 ccagagtatc tgcaatgcct ggcttccctc gccagggcac agaatgatag cgaagcgtta
     5101 gccgttcacc tgcaacgcgg cgcggttaat cttcctgatt tcgcctgggc gcgccagctc
     5161 aacggcgaag ggatgcgcga actgctgcaa cagcctggct atattcaggc tggttatagc
     5221 ttgttgaatg cgccggttgc cgctcgctgg caacggaaaa ttctcgacac attagcgact
     5281 tatcatgagc aacatcgcga tgaacctggg cctgggcgcg aacgtctgcg acgtatggcg
     5341 ttgccaatgg aagatgaagc gctagtactg ttgctgattg aaaagatgcg cgaaagcggc
     5401 gacatcctca gtcatcacgg ctggctgcat ctgccggatc acaaagcggg cttcagcgaa
     5461 gagcagcagg ccatctggca aaaagcagag ccgctgtttg gtgacgagcc gtggtgggtg
     5521 cgtgatctgg caaaagagac gggtaccgac gagcaggcaa tgcgcctgac gctacgccag
     5581 gcggcgcagc aaggaataat taccgcgatc gtcaaagatc gttattaccg taacgatcgg
     5641 attgtcgaat ttgccaatat gatccgcgat ctcgatcagg agtgtggttc aacctgcgcg
     5701 gcggatttcc gcgatcgctt aggcgtaggc cgaaagctgg caattcagat tctggaatat
     5761 tttgaccgca ttggctttac gcgtcgtcgt ggaaatgatc atttattgcg cgacgcatta
     5821 ttatttccgg aaaaataagg aaatgattaa ttaattttta aaataaatta atacaaattt
     5881 cttatgattt t
//
LOCUS       DABSYT020000181         5706 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.182, whole genome shotgun sequence.
ACCESSION   DABSYT020000181 DABSYT020000000
VERSION     DABSYT020000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.182"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..449
                     /gene="cesT"
                     /locus_tag="ID725_RS23675"
                     /old_locus_tag="pgaptmp_004735"
     CDS             <1..449
                     /gene="cesT"
                     /locus_tag="ID725_RS23675"
                     /old_locus_tag="pgaptmp_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HGV7853170.1"
                     /translation="LEKFAEKIGIGSISFNENRLCSFAIDEIYYISLSDANDEYMMIY
                     GVCGKFPTDNSNFALEILNANLWFAENGGPYLCYEAGAQSLLLALRFPLDDATPEKLE
                     NEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSSDNKHYYAGR"
     gene            509..3313
                     /gene="eae"
                     /locus_tag="ID725_RS23680"
                     /old_locus_tag="pgaptmp_004736"
     CDS             509..3313
                     /gene="eae"
                     /locus_tag="ID725_RS23680"
                     /old_locus_tag="pgaptmp_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HGV7853171.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTV
                     KVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVS
                     DGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYS
                     ENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMS
                     ICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQRSGVSSTYNLIT
                     QNPLPGVNVNTPNVYAVCVE"
     gene            complement(3577..4797)
                     /gene="escD"
                     /locus_tag="ID725_RS23685"
                     /old_locus_tag="pgaptmp_004737"
     CDS             complement(3577..4797)
                     /gene="escD"
                     /locus_tag="ID725_RS23685"
                     /old_locus_tag="pgaptmp_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HGV7853172.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DVLTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNAKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
     gene            4940..>5706
                     /gene="sepL"
                     /locus_tag="ID725_RS23690"
                     /old_locus_tag="pgaptmp_004738"
     CDS             4940..>5706
                     /gene="sepL"
                     /locus_tag="ID725_RS23690"
                     /old_locus_tag="pgaptmp_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HGV7853173.1"
                     /translation="MANGIEFNQNPASVFNSNSLDFELESQQLTQKNSSNISSPLINL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGSTIEKLLNDMQELLTLTDSDKIKE
                     LSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLLGE
                     DDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKH
                     LKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIF"
BASE COUNT         1722 a         1049 c         1224 g         1711 t
ORIGIN      
        1 tattagaaaa atttgcagaa aaaattggta ttggatctat ttcatttaat gaaaacagat
       61 tgtgttcttt tgctattgat gaaatttatt atatttcgtt atctgatgcc aatgacgaat
      121 atatgatgat ttatggtgtc tgtgggaaat tcccgacaga taactctaac ttcgctcttg
      181 agattttgaa tgcaaactta tggtttgcag agaatggtgg gccatatctg tgctatgagg
      241 ctggagcaca atcgctgttg ttagcgttac gtttccctct cgatgatgct acccctgaaa
      301 aactcgagaa tgaaatagaa gtcgttgtta agtcaatgga aaacctgtat ttggtattac
      361 ataatcaggg aataacatta gaaaacgaac atatgaaaat agaggaaatc agttcaagcg
      421 acaataaaca ttattacgcc ggaagataaa atccgatcta ttaatataat ttatttctca
      481 ttctaactca ttgtggtgga gccataacat gattactcat ggttgttata cccggacccg
      541 gcacaagcat aagctaaaaa aaacattgat tatgcttagt gctggtttag gattgttttt
      601 ttatgttaat cagaattcat ttgcaaatgg tgaaaattat tttaaattgg gttcggattc
      661 aaaactgtta actcatgata gctatcagaa tcgccttttt tatacgttga aaactggtga
      721 aactgttgcc gatctttcta aatcgcaaga tattaattta tcgacgattt ggtcgttgaa
      781 taagcattta tacagttctg aaagcgaaat gatgaaggcc gcgcctggtc agcagatcat
      841 tttgccactc aaaaaacttc cctttgaata cagtgcacta ccacttttag gttcggcacc
      901 tcttgttgct gcaggtggtg ttgctggtca cacgaataaa ctgactaaaa tgtccccgga
      961 cgtgaccaaa agcaacatga ccgatgacaa ggcattaaat tatgcggcac aacaggcggc
     1021 gagtctcggt agccagcttc agtcgcgatc tctgaacggc gattacgcga aagataccgc
     1081 tcttggtatc gctggtaacc aggcttcgtc acagttgcag gcctggttac aacattatgg
     1141 aacggcagag gttaatctgc agagtggtaa taactttgac ggtagttcac tggacttctt
     1201 attaccgttc tatgattccg aaaaaatgct ggcatttggt caggtcggag cgcgttacat
     1261 tgactcccgc tttacggcaa atttaggtgc gggtcagcgt tttttccttc ctgcaaacat
     1321 gttgggctat aacgtcttca ttgatcagga tttttctggt gataataccc gtttaggtat
     1381 tggtggcgaa tactggcgag actatttcaa aagtagcgtt aacggctatt tccgcatgag
     1441 cggctggcat gagtcataca ataagaaaga ctatgatgag cgcccagcaa atggcttcga
     1501 tatccgtttt aatggctatc taccgtcata tccggcatta ggcgccaagc tgatatatga
     1561 gcagtattat ggtgataatg ttgctttgtt taattctgat aagctgcagt cgaatcctgg
     1621 tgcggcgacc gttggtgtaa actatactcc gattcctctg gtgacgatgg ggatcgatta
     1681 ccgtcatggt acgggtaatg aaaatgatct cctttactca atgcagttcc gttatcagtt
     1741 tgataaatcg tggtctcagc aaattgaacc acagtatgtt aacgagttaa gaacattatc
     1801 aggcagccgt tacgatctgg ttcagcgtaa taacaatatt attctggagt acaagaagca
     1861 ggatattctt tctctgaata ttccgcatga tattaatggt actgaacaca gtacgcagaa
     1921 gattcagttg atcgttaaga gcaaatacgg tctggatcgt atcgtctggg atgatagtgc
     1981 attacgcagt cagggcggtc agattcagca tagcggaagc caaagcgcac aagactacca
     2041 ggctattttg cctgcttatg tgcaaggtgg cagcaatatt tataaagtga cggctcgcgc
     2101 ctatgaccgt aatggcaata gctctaacaa tgtacagctt actattaccg ttctgtcgaa
     2161 tggtcaagtt gtcgaccagg ttggggtaac ggactttacg gcggataaga cttcggctaa
     2221 agcggataac gccgatacca ttacttatac cgcgacggtg aaaaagaatg gggtagctca
     2281 ggctaatgtc cctgtttcat ttaatattgt ttcaggaact gcaactcttg gggcaaatag
     2341 tgccaaaacg gatgctaacg gtaaggcaac cgtaacgttg aagtcgagta cgccaggaca
     2401 ggtcgtcgtg tctgctaaaa ccgcggagat gacttcagca cttaatgcca gtgcggttat
     2461 attttttgat caaaccaagg ccagcattac tgagattaag gctgataaga caactgcagt
     2521 agcaaatggt aaggatgcta ttaaatatac tgtaaaagtt atgaaaaacg gtcagccagt
     2581 taataatcaa tccgttacat tctcaacaaa ctttgggatg ttcaacggta agtctcaaac
     2641 gcaagcaacc acgggaaatg atggtcgtgc gacgataaca ctaacttcca gttccgccgg
     2701 taaagcgact gttagtgcga cagtcagtga tggggctgag gttaaagcga ctgaggtcac
     2761 tttttttgat gaactgaaaa ttgacaacaa ggttgatatt attggtaaca atgtcagagg
     2821 cgagttgcct aatatttggc tgcaatatgg tcagtttaaa ctgaaagcaa gcggtggtga
     2881 tggtacatat tcatggtatt cagaaaatac cagtatcgcg actgtcgatg catcagggaa
     2941 agtcactttg aatggtaaag gcagtgtcgt aattaaagcc acatctggtg ataagcaaac
     3001 agtaagttac actataaaag caccgtcgta tatgataaaa gtggataagc aagcctatta
     3061 tgctgatgct atgtccattt gcaaaaattt attaccatcc acacagacgg tattgtcaga
     3121 tatttatgac tcatgggggg ctgcaaataa atatagccat tatagttcta tgaactcaat
     3181 aactgcttgg attaaacaga catctagtga gcagcgttct ggagtatcaa gcacttataa
     3241 cctaataaca caaaaccctc ttcctggggt taatgttaat actccaaatg tctatgcggt
     3301 ttgtgtagaa taattccata accaccccgg ctaaaatatg tattgtttta gtcggggcat
     3361 aattatttct tcttaagaaa taacctctta taatcaaatc tactactggt ctttttatct
     3421 gcttaatagg tctctttcaa agagacacat tcacgttttc tcatgtaggt tgatccaacc
     3481 acgctgatac caaagctgaa tcacatcaag caacaactat gctcacaaca tccacacaat
     3541 aaaaaaccct ccgaagaggg ggaagaggat aagaaattaa tacgacagtg gaatatgtat
     3601 atattcgtct gggcgggaga tgttgatacc atctttagga tcaatcgcgt ctatgacata
     3661 gcctcctttc agaatgctac caatccccag gctggtgcca ttatcaagag caattgttgg
     3721 attcttcatc gtaccgctga ttctgagtat ttttccgggg aaaatatctg ctgaaatgta
     3781 cggctgaatg ttctgaaaga gtatcttggc gttactattt ttgttcattg catttataat
     3841 ggcaaggatc ttactctcat tctgttgaga aacttccccc gcaacaatga tgacatcatt
     3901 ctttttgaaa atattgacat agtttattag tttattcttt ttaaactctg atgccagttc
     3961 attgatttta ttgttactgt tgttgtgtat ttcccatcct gcaactcctg gcgtattcag
     4021 taacagattt tcaacctcgc tccatttggg tgaaggggga atgtatcccg ataacagaaa
     4081 gaatccaggc ttattacctt ttgtaataat gatatctttg taaccgtatt ccgttaagac
     4141 atcattaatt gcggatatga tttggttatt gcaaatgata ttattgtaat aaaaaatatt
     4201 tttttctttt aggtaattaa aaaatggagt taaatccgtg gttgattcgc aacgcccata
     4261 aagcgcgata tttttcccat gccataccag agtgattgca tgaagtttgt tttcttttag
     4321 ctgctgatat acatcagctc tggtaaacgt tgggggttca gcttttgggg aaagcaaaac
     4381 gtaactgccg atggcaccag acaacgcaaa agtagcacca atcacgctcg ccaggataag
     4441 gcgttttttg cgtcgacttt tggcagagaa cctttctggc acatcagaca atggcaagga
     4501 atggtcaata tcaccaatga tgaagcaaca tcccgctata tcaataacct ttccgacagg
     4561 taatggttta tctgcttcat agggcgtggg ttgcccatca atccaaaatt caaccggaga
     4621 taaaaggaaa acaccttcct cacgtatatc aagtaaaaac tgattgaggc cttgttcaag
     4681 gggaaagtaa acaatatcgt tatcttccgt acctagcgtt aggtttccca tcggaagttg
     4741 taattcccga ttacgcatcg caccattgag aagttttatt ttatatgagg ataacatttt
     4801 taaattttat tcatcctggt ggttgaacaa tgagaaaaaa tatggtgaac ttacatcgtc
     4861 taagaataga gtagaaagga agctgttgta cagtactatt taatggaata ttcataatta
     4921 atgattacgt gagtttccaa tggctaatgg tattgaattt aatcaaaacc ccgcatctgt
     4981 ttttaattct aattcattag attttgaatt agaatctcag caattaacgc aaaaaaattc
     5041 ttctaatatt tcttcgccat taataaattt gcaaaatgaa ttagcaatga ttactagttc
     5101 atcgctttct gaaacgattg aagggctaag cctgggatat cgcaaaggta gcgcaaggaa
     5161 agaagaggaa ggctcgacaa tcgagaagct gctcaatgat atgcaagagc tgcttactct
     5221 taccgatagt gataaaataa aagaactatc attaaaaaac agcgggctgc tggaacaaca
     5281 cgatcctact ttggcgatgt ttggcaacat gccaaagggg gaaattgttg cgctaatatc
     5341 ttctttattg caatctaagt ttgttaagat tgaattaaaa aagaaatatg ccaggttatt
     5401 attagattta ttaggcgaag atgattggga gctggctctg ctttcctggt taggggtggg
     5461 tgagttaaat caggaaggta tccagaagat caagaagctt tatgaaaagg ctaaggatga
     5521 ggattctgaa aatggcgcct ctttacttga ctggtttatg gagattaagg atcttcccga
     5581 gcgtgagaag cacttaaaag tcattattag ggcgctgtcg ttcgatctct cttatatgtc
     5641 ttcttttgaa gacaaagtaa agacatcttc aattattagt gatttatgca gggtaatcat
     5701 tttttt
//
LOCUS       DABSYT020000182         5519 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.183, whole genome shotgun sequence.
ACCESSION   DABSYT020000182 DABSYT020000000
VERSION     DABSYT020000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.183"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..491
                     /locus_tag="ID725_RS23695"
                     /old_locus_tag="pgaptmp_004739"
     CDS             <1..491
                     /locus_tag="ID725_RS23695"
                     /old_locus_tag="pgaptmp_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853174.1"
                     /translation="SRVQQSAQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSV
                     AVSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEK
                     VKTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEK
                     LG"
     gene            515..1729
                     /locus_tag="ID725_RS23700"
                     /old_locus_tag="pgaptmp_004740"
     CDS             515..1729
                     /locus_tag="ID725_RS23700"
                     /old_locus_tag="pgaptmp_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HGV7853175.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            1785..2174
                     /locus_tag="ID725_RS23705"
                     /old_locus_tag="pgaptmp_004741"
     CDS             1785..2174
                     /locus_tag="ID725_RS23705"
                     /old_locus_tag="pgaptmp_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853176.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            2224..2685
                     /locus_tag="ID725_RS23710"
                     /old_locus_tag="pgaptmp_004742"
     CDS             2224..2685
                     /locus_tag="ID725_RS23710"
                     /old_locus_tag="pgaptmp_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853177.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAALAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            2669..3232
                     /locus_tag="ID725_RS23715"
                     /old_locus_tag="pgaptmp_004743"
     CDS             2669..3232
                     /locus_tag="ID725_RS23715"
                     /old_locus_tag="pgaptmp_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853178.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFF"
     gene            3232..3882
                     /locus_tag="ID725_RS23720"
                     /old_locus_tag="pgaptmp_004744"
     CDS             3232..3882
                     /locus_tag="ID725_RS23720"
                     /old_locus_tag="pgaptmp_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HGV7853179.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVEWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            3879..>5519
                     /locus_tag="ID725_RS23725"
                     /old_locus_tag="pgaptmp_004745"
     CDS             3879..>5519
                     /locus_tag="ID725_RS23725"
                     /old_locus_tag="pgaptmp_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGV7853180.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTVVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGDTGERGPKGDQGDMGPKGEKG
                     DPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGPAGPRGERGETGPQGPRG
                     EPGPAGSAANVADATTAQKGIVQLSSATDSDDETKAATPKAVKAAMDVANEAKTKAEE
                     AAAGGGVPGPKGDKGDTGPAGPAGPKGDKGERGDTGPVGATGERGPAGDAGPAGPQGP
                     KGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGE"
BASE COUNT         1349 a         1285 c         1736 g         1149 t
ORIGIN      
        1 agtcccgtgt tcagcaatca gcacagcagg cagaaggtaa tggtggtgag gatttatcac
       61 cggttcttga tgcgatgaat gccgtgccgg tgctgaaaac gtggcaggag tccgatccag
      121 atcgcttctc ggttgctgta tccatcgacg ggaagctcca gaatgacccc gcatggaaag
      181 acaaaacgct cactgaacgt ttcgctgaag tggcccgtcg tacgcaggtt gctttcggtg
      241 aagtcagtga gtcgtctgct gacaacaagg cagacaaaac ggatatccgg aaaacggcgg
      301 aagagaaagt gaagaccgct gaacaggagc aggcagtacc tgcttccccg tcagatttag
      361 gcaccacggc ttccgtcgga accggtgata attttgaacg gttacttggc gcttctcatt
      421 cagaggcaga ggcgattatg cgcggtatga cgaatgctga aatagacgcg cttctggaga
      481 agctcgggta acttactgaa ggagaactga agtaatgacg actgtaacat cagcccaggc
      541 gaataagctg tatcaggtgg cgctttttac cgctgccaac cgcaaccgct cgatggtaaa
      601 tatcctcact gaacagcagg aagcgccaaa agcggtttcg ccggacaaga aaagcacgaa
      661 gcagaccagc gcgggtgcgc cggttgtccg tatcacagac cttaacaaac aggccggtga
      721 tgaagtgacc ttcagcatca tgcacaaact ctcaaaacgt ccgacgatgg gagatgagcg
      781 tgttgaaggt cgtggtgagg atctcagcca tgctgacttc tccctgaaaa tcaatcaggg
      841 acgtcacctg gtggatgcag gcggacgtat gagtcagcag cgcacgaagt ttaacctggc
      901 atcctcagcc agaacgcttc tggggacgta ctttaatgac ctgcaggacc agtgtgcgat
      961 agtgcatctt gctggagctc gtggtgattt tgttgctgac gacactattc tgccgacagc
     1021 ggagcaccct gaattcaaaa aaatcatgat caacgatgta ctgcctccga cacatgaccg
     1081 tcactttttt ggcggtgatg cgacaagctt tgagcagatt gaagcggcag atattttttc
     1141 tattggcctg gtggacaatc tctccctgtt cattgacgaa atggcgcatc cgttacagcc
     1201 ggttcgtctg tccggtgatg aacttcacgg agaagatcca tattacgtcc tgtacgtcac
     1261 gccgcgtcag tggaatgact ggtacacctc gacgtccggt aaggactgga accagatgat
     1321 ggttcgtgcc gtgaaccgtg caaaaggttt taatcatccg ctgttcaaag gtgaatgtgc
     1381 gatgtggcgc aatatcctgg ttcgtaagta tgcgggtatg ccgatccgtt tctatcaggg
     1441 gtcaaaggtt ctggtatcag agaataacct gacggcaacc acgaaagagg tcgctgctgc
     1501 aaccaatatt gaccgcgcca tgttactggg ggctcaggcg ctggcaaatg cttacggtca
     1561 gaaggcgggc ggtcacttca acatggttga gaagaaaacg gatatggata accgtactga
     1621 gatagcaatc agctggatca acggtctgaa aaaaatccgt ttccccgaga agagcggcaa
     1681 gatgcaggat cacggcgtga ttgccgttga tacagcagtg aagctctgat tttttccttt
     1741 ccctatgccg ggttttcgcc cggctttttc aggagtcatt aattatggca aagactatcc
     1801 ttgccccgtc actgagtgaa cgggtctata cgggtacgca cggtaatgag tcggtggcag
     1861 aaggcgtatt tacggtgaat gctgcggaag cggacagtgt tattcatctt ctctcactgc
     1921 cagtgggcat ccgtatcaac tcactccaac tggtttcaac gggtggtctg ggtactgcaa
     1981 ccgtcagcat taagtccggt gagcatgctc tcatcgataa cagcgaagct gtttctgcaa
     2041 aatttgccag atatgtgcca gtggagccgt acaccacaca gcgtgacggg gagctggtta
     2101 ctgtcaccat taagactgcc gctgcaaccg gcaccctgaa tgttctgctg cgttataccg
     2161 tggtgggata ctgattaaaa ccttccggcc cgcgtcatgc gggcttttta tccggggaat
     2221 tatatgagtg agaaaattgc cgttgtctat atcggcccaa aacccgtgaa aaaggacacc
     2281 attaccggaa gtcgcacgct gttcccacgt cttgagccgg tgcatgttga cagcgcgatg
     2341 gcctggcaac tgctggggtt tccggatgtc tgggttcgtc atgaagagct tgatgatgtt
     2401 ctgaaaaagc aacaacagaa tgagcagttg cggcaggcac agcaggcgca ggaaagagtg
     2461 cttgctgcgc tggcagaagc ggagaacagt tttgttgttt ctgttaacgg gcaggaggtg
     2521 gatttaagta agctcacctc agcacggctg gcgacgctgt gtgaggcaga agagctggat
     2581 attcacaaag acccgaaaga aacggctgag gcattccgta tccgggtgcg tgaggcattt
     2641 cgccgtcgtg ttgcggagac tgaacagcat ggcggaactg agtgattttt taccgtatgt
     2701 ccgtcgtcat atcagcggtc cactgaacat tatgatgacg gatgctctgt caatggctgc
     2761 cgtggcattc agccgccagt cgttggtgtg ccgtcgggag gttactgttg taccagtagc
     2821 aggaaaagaa atcgtgcttc cgtatgacaa agatgatgag gagtgcgttc atatcatccg
     2881 tatctctgac gataatcatg agctttttgt cggtcgggat gtggatatca gctccggacg
     2941 ctccctgcga tttgcctgtt ctcccggtga ggtgagcgtg ctttatgccg tcgctccgaa
     3001 agccggacgc agccagatac cggatgaact cctcacatgg cctgaagaag tggctgcggg
     3061 ggcacttgag cggttgttca tgcagactgg tgtttcatgg tcagatccgt tacgcgcaca
     3121 gtatttttct gtgcagtttt ctgaggggat ccgtcgggca tatcgtcata cactggcgac
     3181 aagcccgtac tcttcatacc gcaaccctgt acgcaggcag aggtttttct gatgacgacg
     3241 attacagaaa tcatcggacg tgtgaataca caactggttg acccgatgat ggttcgctgg
     3301 cccctgcagg aattgtgcga ttattacaat gatgctgtga gggcagtgat tctggcgaga
     3361 ccggatgctg gcgcaagcct ggaaacaata agttgtgttc ctggcgcccg tcaggttttg
     3421 cccgatggtg taatacaact tcttgacgtg atatgcctca gtgacggtag tgcagtcaga
     3481 ccattatccc gggaggtgct ggatgcgcag tatcctgagt ggcccacaat gaagggtatt
     3541 cctgaatgtt ttatcagcaa cgacctgtcc ccgcgcgtat tctggctgtt tcctgctcct
     3601 gacaaagaga taagtattga tgcagtggta agccggatac cggaggcagt gtatgttctg
     3661 acgcaggacg atgatacgcc agttccactg gaagaggctt atgttaaccc actggtggag
     3721 tggatgttgt ttcgcgcttt cagtaaggat gctgccggtg gcgcagaatc ggggctggct
     3781 gcgcagcatt atcagagttt tgttgagcaa cttgggatca aacagggggc agacagtgca
     3841 ttgtctgccc gtaaaaaagt gtttaacgga ggtggagtgt gagtgttgtt gtttcgggga
     3901 cgctgaaatc tcctgatggt gaggcgatat caggagcaaa tattaccctg acggcgctga
     3961 cagtttcacc ggatgcgctc agcggcacca gtgcgtcggc agtgacccgt gaaggtggat
     4021 attacggaat gacgatggat ccgggggagt atgcggtttc ggtgacggtg aaagggaaga
     4081 ctgttgtcta cggacgtgtg cgtattgagg ggaccgaaag tacggtgacg ctcaatatgc
     4141 tgttacgccg cagtcttgtt gaggttagca tacccggaga actgctgaca gatttccggc
     4201 agatacagaa taatgtggct gatgaccttg ccactattcg tcgcctgaat gaagacacgg
     4261 cgacaaaaaa cactcaggcc acacagtcaa aagaaagtgc agcagccagt gcgaagagtg
     4321 catctgacag tgcaaagacg gcaaccagca gggcggctga agccggacaa aaagcgactg
     4381 atgccactga ggctgcgacc cgtgcagtca cagcagcggg gaatgcagag gaaagctcga
     4441 cccgtgccgg agagtctgaa aaagccgccg gagctgatgc agaaaaagcc agacagcatg
     4501 ctgaaaaggc caggctggcg caggagagcg ccggagagat ccttaagcgg gcagaggctg
     4561 ccactgtcag tgctgaagag gccagacgta tggctgagaa tgcacggggg ccccgggggc
     4621 ctcagggaga aactggtccg aagggggatg tcggtcctaa aggcgaaaca ggtccagtgg
     4681 gccctcaagg gcccgcaggg ccgaaaggtg agcgtggtga cgttggtgct cagggggctg
     4741 tagggcctgc tggtccgcgt ggtgagaagg gcgaacaggg ggagcgagga ccgcagggaa
     4801 taccaggcct gaagggggat accggagagc gggggcctaa aggggaccag ggggatatgg
     4861 ggccaaaagg cgagaaaggt gatccgggag gtcctgcagg cccgcaaggt cctaaaggcg
     4921 aacgaggaga agccggacca cagggaccga tgggagcacg aggtgagcgt ggggagactg
     4981 gcccccgagg tgaacctggt cctgcaggtc cgagaggcga acgaggagag accggacctc
     5041 agggacctcg tggagagcca ggtccggcag gcagcgctgc aaatgtggct gatgcaacga
     5101 cggcacagaa gggaattgtg cagttaagca gcgcaacgga cagtgatgat gaaacgaagg
     5161 ctgccacccc gaaagcggtg aaagcggcaa tggatgtggc aaatgaagcg aaaacaaagg
     5221 cagaagaggc tgcagcagga ggtggtgttc ccggtccgaa aggagataaa ggggacacgg
     5281 ggccagcagg tccggctggg ccgaagggtg ataagggaga gcgcggtgac accggccctg
     5341 tcggggcaac cggcgaacgg ggaccggcag gtgatgctgg tccggcaggc ccgcaggggc
     5401 cgaaaggtga caggggagag cggggagaga ccggtctgac gggaaatgca ggtccacagg
     5461 gtccaaaggg agataccggt gcggcaggcc cggcaggccc acagggaccg aaaggagaa
//
LOCUS       DABSYT020000183         5482 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.184, whole genome shotgun sequence.
ACCESSION   DABSYT020000183 DABSYT020000000
VERSION     DABSYT020000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.184"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            232..1290
                     /gene="citC"
                     /locus_tag="ID725_RS23730"
                     /old_locus_tag="pgaptmp_004746"
     CDS             232..1290
                     /gene="citC"
                     /locus_tag="ID725_RS23730"
                     /old_locus_tag="pgaptmp_004746"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGV7853181.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1305..1601
                     /gene="citD"
                     /locus_tag="ID725_RS23735"
                     /old_locus_tag="pgaptmp_004747"
     CDS             1305..1601
                     /gene="citD"
                     /locus_tag="ID725_RS23735"
                     /old_locus_tag="pgaptmp_004747"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGV7853182.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1598..2506
                     /gene="citE"
                     /locus_tag="ID725_RS23740"
                     /old_locus_tag="pgaptmp_004748"
     CDS             1598..2506
                     /gene="citE"
                     /locus_tag="ID725_RS23740"
                     /old_locus_tag="pgaptmp_004748"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGV7853183.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2517..4049
                     /gene="citF"
                     /locus_tag="ID725_RS23745"
                     /old_locus_tag="pgaptmp_004749"
     CDS             2517..4049
                     /gene="citF"
                     /locus_tag="ID725_RS23745"
                     /old_locus_tag="pgaptmp_004749"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGV7853184.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4053..4604
                     /gene="citX"
                     /locus_tag="ID725_RS23750"
                     /old_locus_tag="pgaptmp_004750"
     CDS             4053..4604
                     /gene="citX"
                     /locus_tag="ID725_RS23750"
                     /old_locus_tag="pgaptmp_004750"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGV7853185.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4579..5457
                     /gene="citG"
                     /locus_tag="ID725_RS23755"
                     /old_locus_tag="pgaptmp_004751"
     CDS             4579..5457
                     /gene="citG"
                     /locus_tag="ID725_RS23755"
                     /old_locus_tag="pgaptmp_004751"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGV7853186.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
BASE COUNT         1284 a         1533 c         1433 g         1232 t
ORIGIN      
        1 ttaattaaaa aacttaaaac cattaaataa ataaaaaacc actaactcta tgtgaaataa
       61 atcaaaattt cacgccgaaa tactccttag gatgtatagc gaaaggagaa aaagatatac
      121 ctcgatcacc cccctttctc ccaagtgaaa ataaaaggtt atcagtttgc aacattgaac
      181 aacattcgtt gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc
      241 aatgatattt tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa
      301 ttcctgcatg aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc
      361 cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgct
      421 atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac
      481 ctcgcctatg agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg
      541 ctgttccgcc agtgcggttt ttccacgctg accagcgtac ccggcgtgat ggtgctgatg
      601 gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca
      661 gggaacaaga ttggctgcat tgcgatgaac gccaatccct ttacgaatgg tcaccgttat
      721 ctgattcaac aagctgcggc acagtgcgac tggttgcatc tgtttttagt caaagaagat
      781 tcttcacgct tcccctatga agaccggctg gatctggtgt taaaaggcac cgccgatatt
      841 ccacgcctga ctgtgcatcg cggctccgaa tacatcatct cccgcgctac gttcccttgc
      901 tacttcatta aagaacagag cgtcattaac cattgttaca ccgaaattga tctgaagatt
      961 ttccgtcagt acctcgctcc cgcgctgggt gtaactcacc gctttgtcgg tactgaaccc
     1021 ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact
     1081 atctccgcag cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg
     1141 atatccgctt cccgggtacg tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg
     1201 ctggtccctg cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac
     1261 gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc
     1321 ccgccgttgc aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata
     1381 cgcaggatat cgacctgcaa atcaatagca gcgttgagaa acagtttggc gatgcaattc
     1441 gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg
     1501 atgataaagg cgcactggac tgcattttac gtgcacgact ggaagccctg ctggcacgcg
     1561 ccagcggtat cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca
     1621 acgtaaaact cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt
     1681 cagcaactcc ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgtggc
     1741 attacgtgaa aaagacaccg cccgccgcat ggtttatcac gcgctgcaac atccgctgta
     1801 tcgcgatatt gaaaccattg tgcgtgtaaa cgcgctggat tccgaatggg gtgttaacga
     1861 cctggaagcc gtcgttcgcg gtggtgcgga cgttgtacgt ctgccgaaaa ccgataccgc
     1921 tcaggatgtt ctggatatcg aaaaagagat cctgcgtata gaaaaagcct gtggtcgtga
     1981 acccggcagc accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt
     2041 ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt
     2101 gcgcaacctg cgtacagaac gctccccgga aggaactgaa ctgctgttcg cacgctgttc
     2161 cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc
     2221 taacaacgaa gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg
     2281 caaatcgctg atcaacccgc gtcagattga tctgctgcac aacctctacg caccgaccca
     2341 gaaagaagtg gatcacgccc gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg
     2401 cctcggcgtg gtttccctga acggcaagat ggtggacggt ccggttatcg atcgtgcccg
     2461 tctggtgctc tcccgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga
     2521 cgcagaaaat tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcgctg
     2581 aatgcgatct tgccgctttc cagaactcac caaagcaaac ctaccaggct gaaaaagcgc
     2641 gcgatcgcaa actgtgcgcc aacctggaag aagcgattcg tcgctctggt ttacaggacg
     2701 gcatgacggt ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga
     2761 tggacgtcat cgcgaagatg ggctttaaaa acctgatcct ggcgtccagc tccctgagtg
     2821 attgccatgc gccgctggta gaacacattc gccagggcgt ggttacccgc atttatacct
     2881 ccggcctgcg tggtccactg gcggaagaga tctcccgtgg tctgctggcg gaaccggtgc
     2941 aaatccactc tcacggcgga cgtgtgcatc tggtacagag cggcgaactg aatatcgacg
     3001 tggctttcct cggcgtcccg tcctgtgatg aattcggtaa tgccaacggc tacaccggta
     3061 aagcctgctg cggctccctc ggctatgcaa tggttgatgc cgacaacgca aaacaggttg
     3121 tgatgcttac cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc
     3181 aggttgattt aatcgtcaaa gttgatcgcg ttggcgatgc tgcaaaaatc ggcgctggtg
     3241 ctacccgtat gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga
     3301 ttgtcaactc aggctacttc aaagaaggtt tctccatgca aaccggcacc ggcggcgcat
     3361 cgctggcggt aacccttttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact
     3421 tcgccctcgg tggtattacc gcgacgatgg ttgatctgca cgaaaaaggt ctgatccgca
     3481 aactgctgga tgtacagagc tttgacagcc acgctgcgca atcgctggcc cgtaacccca
     3541 atcacatcga aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggt gcatcggttg
     3601 atcgtctcga cgtggtggta ctgagcgcgc tggaaattga cacccagttc aacgttaacg
     3661 tgctgaccgg ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc gataccgcga
     3721 ttgcctctgc gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt ccgactctgg
     3781 tggataacgt gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc
     3841 acggtatcgc agttaacccg gcacgtccgg aactggcaga acgtctgcag gaagcgggca
     3901 ttaaagtggt ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac
     3961 agccgattga attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga
     4021 tcgatgttgt gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc
     4081 accatgcggt gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac
     4141 acgtctggct caagcgccat cctgttccac tggtctcctt taccgtggtt gcgcctgggc
     4201 cgattaaaga cagtgaggtc acacgccgaa tttttaatca tggcgtgaca gccttgcgtg
     4261 ccttagccgc aaaacagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg
     4321 ggccggaggg tatgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg
     4381 agcttgaaca tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag
     4441 gcgaaattct ctcccgccgc gactattcac tgccgcctcg ccgctgcctg ttgtgcgagc
     4501 aaagcgcagc cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca
     4561 tggaggcact gctgaacgat gtcgatgcct gcaacgttaa ctaaaaccac aaagcttgcg
     4621 acgtcattaa tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat
     4681 ctgtcaccga aaccaggtct cgtggatcgc attaactgcg gtgcgcataa agatatggcg
     4741 ctggaagatt tccaccgcag cgcgctggcg attcagggct ggctaccccg tttcattgaa
     4801 tttggtgcct gtagtgcgga aatggcacca gaagcggtac tccacggatt acgcccaatt
     4861 ggtatggctt gcgaaggtga tatgttccgc gccaccgcgg gcgtaaacac gcataagggc
     4921 agcatttttt ctttagggct actgtgtgcg gcaattggcc gtttgcttca gctcaaccag
     4981 ttgctaacgc caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg cctgaccgat
     5041 cgcgaactgc gtaccaataa ttcacaactg acggcaggtc aacggttgta ccagcagctt
     5101 ggccttaccg gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat caaccacgcc
     5161 ttgccgcatt acctcactct gctggatcag gggttagatc ctgaactggc attgctcgat
     5221 accttgctcc tactgatggc gatcaacggc gataccaacg ttgcttcgcg cggtggcgag
     5281 gggggcctgc gctggctaca gcgcgaggcg caaacattat tgcaaaaagg gggcattcga
     5341 acccccgcag atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc
     5401 agtccaggcg gcagtgccga cctgctgatc cttacctggt ttttagcaca gatttaatta
     5461 tttaagaact tgataaattt gg
//
LOCUS       DABSYT020000184         5430 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.185, whole genome shotgun sequence.
ACCESSION   DABSYT020000184 DABSYT020000000
VERSION     DABSYT020000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.185"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..384
                     /locus_tag="ID725_RS23760"
                     /old_locus_tag="pgaptmp_004752"
     CDS             <1..384
                     /locus_tag="ID725_RS23760"
                     /old_locus_tag="pgaptmp_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGV7853187.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="ID725_RS23765"
                     /old_locus_tag="pgaptmp_004753"
     CDS             419..1117
                     /locus_tag="ID725_RS23765"
                     /old_locus_tag="pgaptmp_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGV7853188.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="ID725_RS23770"
                     /old_locus_tag="pgaptmp_004754"
     CDS             1139..1363
                     /locus_tag="ID725_RS23770"
                     /old_locus_tag="pgaptmp_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853189.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="ID725_RS23775"
                     /old_locus_tag="pgaptmp_004755"
     CDS             1360..1716
                     /locus_tag="ID725_RS23775"
                     /old_locus_tag="pgaptmp_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HGV7853190.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="ID725_RS23780"
                     /old_locus_tag="pgaptmp_004756"
     CDS             1749..1901
                     /locus_tag="ID725_RS23780"
                     /old_locus_tag="pgaptmp_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853191.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="ID725_RS23785"
                     /old_locus_tag="pgaptmp_004757"
     CDS             1898..2209
                     /locus_tag="ID725_RS23785"
                     /old_locus_tag="pgaptmp_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853192.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2899
                     /locus_tag="ID725_RS23790"
                     /old_locus_tag="pgaptmp_004758"
     CDS             2336..2899
                     /locus_tag="ID725_RS23790"
                     /old_locus_tag="pgaptmp_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853193.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            3009..3113
                     /locus_tag="ID725_RS23795"
                     /old_locus_tag="pgaptmp_004759"
     CDS             3009..3113
                     /locus_tag="ID725_RS23795"
                     /old_locus_tag="pgaptmp_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853194.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3357..3512
                     /gene="hokD"
                     /locus_tag="ID725_RS23800"
                     /old_locus_tag="pgaptmp_004760"
     CDS             3357..3512
                     /gene="hokD"
                     /locus_tag="ID725_RS23800"
                     /old_locus_tag="pgaptmp_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGV7853195.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3680..3958
                     /locus_tag="ID725_RS23805"
                     /old_locus_tag="pgaptmp_004761"
     CDS             3680..3958
                     /locus_tag="ID725_RS23805"
                     /old_locus_tag="pgaptmp_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853196.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3960..5009
                     /locus_tag="ID725_RS23810"
                     /old_locus_tag="pgaptmp_004762"
     CDS             3960..5009
                     /locus_tag="ID725_RS23810"
                     /old_locus_tag="pgaptmp_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGV7853197.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            5022..5381
                     /locus_tag="ID725_RS23815"
                     /old_locus_tag="pgaptmp_004763"
     CDS             5022..5381
                     /locus_tag="ID725_RS23815"
                     /old_locus_tag="pgaptmp_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGV7853198.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     LVPGERLGIKITELECA"
BASE COUNT         1444 a         1225 c         1521 g         1240 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggtcacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg ggggtaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcagtat cggatgaacg ggcagcctat gaattattta tggagaagcg
     2521 tttcggggaa tctgtagatc gccgcagagc aaaaaatggc gatagagaat acatggcatg
     2581 ggatatggcg cttggctgga ttatctggtg tcaccgcgcc gccatgcttc aggctggaaa
     2641 ctttcgggaa aataagggtt cgtcaaccaa taattttcgg ataatctcgg aaacgtcaac
     2701 caactctccg gcaatccctg atgaggtgtt gtccgcaatc ctgaaggtcg ccaggcttcg
     2761 tgcagatttc gatgcttctg aagtggacag gcgaggtatc ggtagttgtc tggatgaggc
     2821 cgagcaagaa cttatcgtta ccattaacga atacgccagt cagatcgcag tagaagcgac
     2881 acagggggag aaccaatgag ccggccagat gcatttgcag gcgttgctat agctattgct
     2941 tttctagtat atgttatttg tcggtggggg taaaaacgtt cgccgggatt cacaccaagg
     3001 gagggaatat gtcggatgat atttcactgg taatggaagg cgctctggct gttattgctg
     3061 ttgtgggtgt ttactgcctg gttgtgtttt tgatggagcg cctggggaac tgaattcatt
     3121 ccgtatggga attcccatat cgggcaaaac ggtttgctgt aaagcgagag ttaagtagaa
     3181 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ccgtaaggca gacagagaaa
     3241 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     3301 tgcttcacaa acgtaggtta gcctcttacg cgccgaaagg caaggagaag caggttatga
     3361 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     3421 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     3481 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     3541 caccgctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     3601 gttttttctg gtcgttctgg tttacaatcc atccgtcagc ctgaacaact ggcacctgct
     3661 gcgccagcag agaaaacaga tggcgcacga taccaaattt tacaattcgg ataactctgc
     3721 cgcccctgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggtatcagca
     3781 cgacccctgc accgaagaac aggctgaatg gctgattcag tgttaccgca ggcgcggata
     3841 cgaggttaag aaagccctca gtctcgatta tcgtcactgg ataatctccg tcaggcttcc
     3901 ttactccgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
     3961 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
     4021 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
     4081 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
     4141 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
     4201 cactgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
     4261 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     4321 gcgacaacca gttgcgtgac cagacctccg aatcacttga gcaacttgct caccaaaacc
     4381 tgtcagcatg gatgattgac gtcattcgtc acgcaatgaa tggcacacag gagcgtgaat
     4441 tatcgctggc tgaattatcc tggtgggcgg cctgcaatca ggtggtggat gcactacctg
     4501 aggcagtagc gcgtcgttcg ctgggattac cagcggaaaa aatccgctcc gtataccgtg
     4561 agagtgacat cgtaccggga gaacagacag ccatcagcat actgaagcag cgcacaaaaa
     4621 atattgcgct gccacttcac gtccaccagc aacaaaatcc accacagaaa aaaacggttg
     4681 tcagtatcgc cgttgatccg gagtctcctg aatcgttcat gaggcggcct aaacgttgcc
     4741 gctgggttaa tgagaaatac acgcgctggg taaagacaca gccgtgtgcg tgttgtggta
     4801 agcctgctga cgatccgcat cacctgattg gtcatggtca ggggggaatg gggacaaagg
     4861 cccacgatat tttcacgcta ccgttgtgcc gggagcacca caacgaactt catgcagacc
     4921 cgctggagtt tgagaaaaag tacggctctc agattgagtt aatttttcgt tttcttgatc
     4981 acgcctttgc gactggcgtg ctcgggtaaa agaggtgact gatgctcata gatttggttt
     5041 taccttaccc gccgacggtg aacacctact ggcgacgtcg tggcagcaca tattttgtat
     5101 caaaagccgg tgagcgttat cgccgggctg tggcgcttat tgttcgccag cagcggctga
     5161 aattaagcct gtccggaagg ctggcgataa agattattgc cgagccaccg gataagcgcc
     5221 gccgtgacct ggacaatatt ctgaaagcgc cgctggatgc gctgacgcat gcggggttgc
     5281 taatggacga tgagcagttt gatgaaatca atattgtgcg cggtcagctc gttcctggtg
     5341 agcggctggg gataaaaatc acagaactgg agtgcgcatg aataaccact atttacagtt
     5401 tgtgcgtgag ctgctcatta tcgctaccgc
//
LOCUS       DABSYT020000185         5424 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.186, whole genome shotgun sequence.
ACCESSION   DABSYT020000185 DABSYT020000000
VERSION     DABSYT020000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.186"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..845)
                     /locus_tag="ID725_RS23820"
                     /old_locus_tag="pgaptmp_004764"
     CDS             complement(<1..845)
                     /locus_tag="ID725_RS23820"
                     /old_locus_tag="pgaptmp_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGV7853199.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICG"
     gene            1114..1230
                     /locus_tag="ID725_RS23825"
                     /old_locus_tag="pgaptmp_004765"
                     /pseudo
     CDS             1114..1230
                     /locus_tag="ID725_RS23825"
                     /old_locus_tag="pgaptmp_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1335..1411)
                     /locus_tag="ID725_RS23830"
                     /old_locus_tag="pgaptmp_004766"
     tRNA            complement(1335..1411)
                     /locus_tag="ID725_RS23830"
                     /old_locus_tag="pgaptmp_004766"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1375..1377),aa:Arg,seq:tct)
     gene            complement(1425..1501)
                     /locus_tag="ID725_RS23835"
                     /old_locus_tag="pgaptmp_004767"
     tRNA            complement(1425..1501)
                     /locus_tag="ID725_RS23835"
                     /old_locus_tag="pgaptmp_004767"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1465..1467),aa:Arg,seq:tcg)
     gene            complement(1509..1584)
                     /locus_tag="ID725_RS23840"
                     /old_locus_tag="pgaptmp_004768"
     tRNA            complement(1509..1584)
                     /locus_tag="ID725_RS23840"
                     /old_locus_tag="pgaptmp_004768"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1549..1551),aa:Ile,seq:cat)
     gene            complement(1613..2326)
                     /locus_tag="ID725_RS23845"
                     /old_locus_tag="pgaptmp_004769"
     CDS             complement(1613..2326)
                     /locus_tag="ID725_RS23845"
                     /old_locus_tag="pgaptmp_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7853200.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRALFLMH
                     YGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGD
                     TTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHM
                     SESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(2464..2660)
                     /locus_tag="ID725_RS23850"
                     /old_locus_tag="pgaptmp_004770"
                     /pseudo
     CDS             complement(2464..2660)
                     /locus_tag="ID725_RS23850"
                     /old_locus_tag="pgaptmp_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(2947..3765)
                     /locus_tag="ID725_RS23855"
                     /old_locus_tag="pgaptmp_004771"
     CDS             complement(2947..3765)
                     /locus_tag="ID725_RS23855"
                     /old_locus_tag="pgaptmp_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGV7853201.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(3917..4288)
                     /locus_tag="ID725_RS23860"
                     /old_locus_tag="pgaptmp_004772"
     CDS             complement(3917..4288)
                     /locus_tag="ID725_RS23860"
                     /old_locus_tag="pgaptmp_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGV7853202.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(4278..4649)
                     /locus_tag="ID725_RS23865"
                     /old_locus_tag="pgaptmp_004773"
     CDS             complement(4278..4649)
                     /locus_tag="ID725_RS23865"
                     /old_locus_tag="pgaptmp_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGV7853203.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(4662..>5424)
                     /locus_tag="ID725_RS23870"
                     /old_locus_tag="pgaptmp_004774"
     CDS             complement(4662..>5424)
                     /locus_tag="ID725_RS23870"
                     /old_locus_tag="pgaptmp_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGV7853204.1"
                     /translation="HGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQN
                     LAAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV
                     YRESDIIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDPESPKSFMKR
                     PKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHH
                     NELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1555 a         1404 c         1145 g         1320 t
ORIGIN      
        1 tcaccacaaa tccacggcac atctgcagca ctgccaccat ggcactgcgc gttaaacacg
       61 gagaggtcag cacgaaactg tgtcagcatg gctgtaaaca gcgcaggttg ctgtgcgtgg
      121 gtggcggcgc tcatgtcaaa ctctccctgc atccagcaca ccgccagcaa cacatttttc
      181 gggttcttct gtaatgcagc tttagtgcgc gcaatcaggt cctgatataa cggtttaccc
      241 acaccccagc gtgccgaatc ctggctggcc cccgtgtccg cactgaatgt cccctcagcg
      301 ccctgggtga atgcagaacc accacgacag catggtacca gcaggatccc cgcgttattc
      361 gggatatacg gaagcagttt tttggcaata tgtaagccct ggccgacaca gccgtactgc
      421 cctttgctca ggtctgcctt cggatgattc agcgtactca tatcctgcac atcatgcagg
      481 cagtggtcgg ccggaatgat gtcgttatac gtacaactct ctccacccgg agttaccgtg
      541 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtaagaatc cggtaacgga
      601 agcccttcac cgtaggccat gccgttggac tgtccggcaa gcacaaccac gtagaaccag
      661 tccggcttag atgaagggcc gacctgtgga tctccttcaa tagccaccgc ctgcatcagt
      721 gtgtacggcg taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt
      781 gtcagctttt ccgcaaggtc tgacggcgac gccgccctga caacatcata gtgtttaaat
      841 gccatgaatc ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc
      901 ggaagttaca ggacaatggc agaagagaga caacagcccg caatacgaaa aaggccgcgc
      961 tattgcgcag agtgattact gtcggatatt attcgccagc tgaaatatta cttcacgttt
     1021 tgttgtttat tccttgccgc ccgcgtctcc ctgcgcgggc tttttttgtc cataagaaag
     1081 cccctccgga gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc
     1141 ccggtgaatt cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt
     1201 gcccctccgc acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca
     1261 gacaacagtc aaccgcctga attgtgagac atttaaaaaa aaggcccgca aaagcgagcc
     1321 agggaaaata agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg
     1381 caattgctct gtccggctga gctaacaacg caggatacag ataatggacc gccttcgggg
     1441 acccgaactc cgcgcaacca gcttcgaaag ctggcgctct ttcctgatga gctaatggcg
     1501 gtatgtgatg gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg
     1561 ctctcaccac tgagctaaag ggctgggtca aaaaataata atcagatgaa aatcaataat
     1621 caagcccttg cctggataca tatctgtctg gcgggaagcc ataatagcgg tgaaatacag
     1681 aaataaagta ggaccagctt gaataaccgc atttttctgc tacagcctgt ccatatccat
     1741 gccgggaaca taacatattg acagcaacac gcatccgctc ttccagcaac aagcgactga
     1801 acatgtgccc ttcatttttc agttttatct ttaacaaact ctcactcata tgcaggcgta
     1861 acgcaatcgc accaagcgtc cagcttgctg atatatctgt ctgaattatc gccctgactt
     1921 tggcacttat actggataaa catccactta aaaataataa tatccgttca tctgattcaa
     1981 acagcgacag gcatgccatc ataagaaaca tatccgtggt atctccggaa aatctctggc
     2041 tggtaattaa agcctcagcc aacgcagggt tgttgggttc cagtgacaga taaagcggaa
     2101 cgtcagtcag atgagttctt gtcagcttat gctgaatttc cagatattga cttactatgg
     2161 aatggtttat atcgaaaatt ttaactttgc cataatgcat aaggaaaagc gccctgatgc
     2221 atttggtggc cagaacgact gagccgggct taagtgacaa cgtatccttt tcaagaaaaa
     2281 tattaattgg ggagcaaacc atgataactg aacagacaac aaccattata attttacttt
     2341 cattagcaat tggttagttc aattatagcc ccaaaaggta aattatcatc aacacataag
     2401 caaaggactg acaggtgccg ctaacaccca ccagccgccc atttaccaca aataaaaaag
     2461 ccttcaggac tgaaggcgtc tgtaacaacc gaactgatag tctgccagac ccgcataacc
     2521 agctgggtca gtattaactg gcagcgctcg cgtgaaaggt acgtattctg cgcaatctcc
     2581 ccgaccgtcg ccggttcggt gacgcttaat tcatcaaaca caactctggc ggtttctgtc
     2641 atatcctgct gtttcagcat gtctttttac cctttccggt taacgtgaca caccaataac
     2701 tcttgtcgaa aaagccagca agctgaaaga cctgtattaa taactaccag cacatttaac
     2761 gcactgcgct actttgcggg cacaaaaaac ccgctcagag gcggggtcaa gctatgcggc
     2821 gaaataacca ctcttaacag catacctgat tttttacgta cgtaaatgct ttgccgtgca
     2881 tatttttcat gcaaatgtca cgtcctgcta tttttcagtc ttataaattt aaaaccatag
     2941 aaaaaatcaa ttatgtttta aaaatggata ggtaaagaat aacaagtgac acagattcaa
     3001 accaaaatgg aaaagggtgg caacccacaa acgcccactc cacatccatg acaatccata
     3061 cacaacacca gataacgtgg caaataatac aagtaaagca ccacctgaat agtgataaaa
     3121 accaaacaac aaagccgcca caattaatgc aaccaatgga gacgttactt ctgatagccg
     3181 tgattgaata taccctctaa ataatgattc ctctgccaga gacacaaaaa acaaattagc
     3241 caatataaac tctggcaacc actcaggaaa atgaatctct ggctttaatc caccaaaaaa
     3301 aacagccaaa aacaggataa gaggaacaga gagcgacaga gccccccact tccacaaaga
     3361 cacttctgat tttacttctt ttttaaacaa agaagatgta cacaggacta acaaaaatgg
     3421 caccagcgct ttatcaaaat taaaatacat tgtataggga gtactttgag ggccaacagt
     3481 aacagaattt agcactacag gattgtgaaa ccctggccat agatggaaaa ataatgctat
     3541 ggctgataaa actatgccaa cttcatatat agatttagcc caggcgttat atttccagtt
     3601 gaacttcaaa ataataaaaa aaacgattgt agcaacaaaa aataacaccg accaatcaat
     3661 aatatcatta agcacagcca ggacaacaga caccgtcaac aatgaaaaag ccactacctt
     3721 atgccaactg agagttgaca gtgacagtac taacacaatc cacataagca ctccttttat
     3781 ttaatgatga agattgatta tcaatatttt caattcacca ggcaacattt tatctacctt
     3841 ccacaataca tgaccatcaa aaaataaaca tgttaattat aaacacagaa aacataaccc
     3901 tcatcactat atatccctac cgcatatcca tatctaaccg gacattcaga gccataagca
     3961 ttccttcaat aataccttcc gctttataaa gccttttacc aataagccca tcagaacatc
     4021 tatgcttacg tgcaagagcc ataaatgtca ttccacctac gtaataatcc accaacaaat
     4081 cgtgcaaata ctgattattc ttgtttaatc gtgccataca accacaaatt atcatggcgt
     4141 catcatcaga acacttagga cgtgatttca ctttagtcgg gattaatcct ttaaaaccag
     4201 cagctatcga gggccatgtt acatcttcat gattatttgc tgcccatgcc ccccatcgct
     4261 caagaaccat ctggatatca cgcgccatcg ttaccacctc tgatttcgta aatcttcacg
     4321 cccagccgtc cacctggcac aggctgaccg cgcacaatat tgatttcatc aaactgctcg
     4381 tcatcaatga gcacttccgc atgcgtcagc gcatccagcg gtgctttcag aatgttgtcc
     4441 aggtcacggc ggcgcttatc cggtggttct gcaataattt ttattgccag ccgtccggac
     4501 aggcttaatt tcagccgctg ctggcgaaca ataagcgcca cagcccggcg ataacgctca
     4561 ccggcttttg atacaaaata tgtgctgcca cgacgtcgcc agtaggtgtt caccgtcggc
     4621 gggtaaggca aaacaaactc tatacgcatc agtaacctct tttacccgag cacgccggtt
     4681 gcaaaagcgt gatcaagaaa acgaaaaatt aaatcaacct gggaaccatg cttttcttcg
     4741 aacgccagcg gatccgcatg aagctcgttg tgatgctccc gacacagcgg tagcgtgaaa
     4801 atatcgtggg attttgttcc cattccaccc tgaccatgac caatcagatg atgaggatcg
     4861 tccgctggct taccacaaca cgcacacggc tgtgtcttta cccagcgtgt gtatttctca
     4921 tttacccagc ggcgacgttt aggtcgtttc atgaaggatt tcggagactc cggatcaacg
     4981 gcaatgctga ccaccgtctt ttcctgtggt gggttctgtt gctggtgggt gtgaggcggt
     5041 agcgcaatat tttttgtgcg ctgcttcagt atgctggtgg cggtctgttc tcccggtatg
     5101 atgtcgcttt cacggtatac ggagcggatt ttttccgccg gtaatcccag agaacgacgc
     5161 gatactgcct caggtaatgc gtccaccacc tgattgcaga ctgcccacca ggataattca
     5221 gccagcgata attcccgttc ctgtatgcca ttcattgcgt ggcggatgac gtcaatcatc
     5281 caggcggcca gattctgttg agcaagttgc tcaagtgatt cggatgtctg gtcgcgcagc
     5341 tggttgtcgc agtgccagca caacaccatc gcgccggtac cgtaacgatg tatgacagtt
     5401 tcgctgtgat gataatcacc atga
//
LOCUS       DABSYT020000186         5203 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.187, whole genome shotgun sequence.
ACCESSION   DABSYT020000186 DABSYT020000000
VERSION     DABSYT020000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.187"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            138..4040
                     /gene="espP"
                     /locus_tag="ID725_RS23875"
                     /old_locus_tag="pgaptmp_004775"
     CDS             138..4040
                     /gene="espP"
                     /locus_tag="ID725_RS23875"
                     /old_locus_tag="pgaptmp_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HGV7853205.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4296..4777)
                     /locus_tag="ID725_RS23880"
                     /old_locus_tag="pgaptmp_004776"
                     /pseudo
     CDS             complement(4296..4777)
                     /locus_tag="ID725_RS23880"
                     /old_locus_tag="pgaptmp_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1572 a         1136 c         1176 g         1319 t
ORIGIN      
        1 tgtttgttat ccgtatgata acaaacacat aaaggagtaa acatttatct ggacgatgat
       61 tacaatgttc acgactgtca gagacacacc tctgatacat tagaaaaacc aatgtttccc
      121 ttaaaaatgg agcttatatg aataaaatat actctcttaa atacagccat attacaggag
      181 ggttaatcgc tgtttctgaa ttatccggca gagtatcatc aagagcaact ggtaagaaaa
      241 aacacaaacg catacttgca ttatgttttt taggcttatt acaatcctca tattcttttg
      301 cgtcacagat ggatatttca aatttctaca tccgtgacta tatggatttt gcacagaaca
      361 agggcatatt tcaggctggc gcaacaaata ttgaaatagt gaagaaagat ggctccaccc
      421 tgaaactacc ggaagtacca tttcctgact tctcaccggt tgcaaacaaa gggtcaacca
      481 catctattgg tggtgcatac agtatcacag ccacacacaa tacgaaaaac caccactcag
      541 ttgcgacgca aaactggggg aacagcacgt acaaacaaac tgactggaat acttcacatc
      601 ctgattttgc agtatcccga cttgacaagt ttgttgttga gacccgaggt gcgactgaag
      661 gcgcagatat ttcgttatca aaacagcagg cacttgaacg ttacggggtt aattataaag
      721 gagaaaagaa acttatcgca ttcagagccg gctctggtgt ggtatccgtt aaaaaaaatg
      781 gacgcataac tccatttaat gaggtttctt ataagccaga aatgttaaat ggctctttcg
      841 ttcacattga tgactggagt ggatggctga tattaaccaa caaccagttt gatgagttta
      901 ataacattgc ctctcagggt gacagcggtt cagcactgtt cgtctatgat aaccaaaaga
      961 aaaagtgggt tgtcgctgga actgtctggg ggatttataa ttacgccaat ggcaaaaacc
     1021 acgcagcata cagtaaatgg aaccagacaa ccattgacaa cctgaagaac aagtattctt
     1081 acaacgtgga tatgtcaggg gctcaggttg caaccattga aaatggaaaa ctgacaggca
     1141 ctggctcaga caccaccgat ataaaaaata aggacttaat atttactggc ggtggagata
     1201 tcctcctgaa atcctctttt gataatggtg ctggcggtct tgtctttaat gataaaaaga
     1261 cctatcgagt aaacggggat gatttcacct ttaaaggtgc cggtgttgat acaagaaacg
     1321 gcagcaccgt tgagtggaat atccggtatg ataataaaga caaccttcac aaaattggtg
     1381 atggcacatt agatgtccga aaaacccaga acaccaacct gaaaacaggt gagggtcttg
     1441 tcattcttgg agctgaaaaa acattcaata atatctacat aaccagtggt gatggaactg
     1501 tccgactgaa tgcagaaaat gcactgtctg gcggtgaata caacggtatt ttctttgcga
     1561 aaaatggcgg aactcttgac ctgaacggat ataatcagtc tttcaataaa attgctgcaa
     1621 ctgattcagg tgctgtaata accaatacgt caaccaaaaa atccatttta tccctgaata
     1681 atactgctga ctatatctat cacggcaaca taaacgggaa tctggacgta cttcagcatc
     1741 atgagacgaa aaaagagaac cgtcgtctta ttcttgatgg gggcgtggac acaacaaatg
     1801 atataagcct gcgtaataca caactgtcca tgcagggaca tgccactgaa catgccattt
     1861 atcgggatgg agctttctct tgttcactac cagctcctat gcgctttttg tgtggcagtg
     1921 attatgttgc aggaatgcaa aatacagaag ctgatgctgt aaaacaaaac ggaaatgcct
     1981 ataaaaccaa caatgctgtc tctgatttat cgcagccaga ctgggaaacc ggaacattca
     2041 gatttggaac gctacatctt gaaaattccg atttttctgt tggtcgtaat gcaaatgtaa
     2101 tcggggacat tcaggccagt aaatcaaaca ttactattgg tgacactaca gcatatattg
     2161 atttgcatgc tggtaaaaat attaccggtg atggttttgg cttccgccag aatattgtgc
     2221 gtggaaactc acaaggagaa acgctgttta caggagggat cacagcagaa gacagcacta
     2281 tcgttattaa agataaagca aaagcattat tttcaaatta tgtatacctg ctgaacacaa
     2341 aagcaaccat agagaacggt gctgatgtga caactcaaag tggtatgttc tccacgagcg
     2401 atatcagcat ctctggtaat ctgtccatga caggcaatcc cgacaaagac aataaattcg
     2461 agccctcaat atatctgaat gatgcttctt atctactgac tgacgactcc gccagactcg
     2521 ttgccaaaaa taaagcatct gtggtgggag atatacactc cactaaaagt gcatccatca
     2581 tgtttggtca tgatgaaagc gacctctcgc agttgtctga cagaacctca aaagggcttg
     2641 cacttggtct tttaggtggc tttgatgtct catatcgcgg ttcagtcaat gccccgtcag
     2701 catctgccac tatgaacaac acctggtggc aactaaccgg agattctgcg ctgaaaacac
     2761 tgaaaagtac aaacagcatg gtctatttca ctgacagcgc aaacaataag aaattccata
     2821 cgctgacggt cgatgagctg gcaaccagca acagcgccta tgcgatgcgt acaaaccttt
     2881 ctgaatcaga caaactggag gtcaaaaaac acttgtctgg tgagaacaat attttactcg
     2941 ttgatttcct tcagaaacca acgcctgaaa aacaactgaa tattgaactg gtaagcgcgc
     3001 caaaagacac caatgaaaat gtctttaaag ccagtaaaca aaccattggt ttcagtgatg
     3061 taacgccggt cattacaacc agggaaaccg atgacaaaat aacatggtca ctgacaggct
     3121 ataacacggt agcaaacaag gaagcaaccc ggaatgccgc cgccctgttc tctgttgact
     3181 ataaagcgtt tctgaacgag gtcaacaacc tgaacaaacg tatgggtgac ctgcgtgata
     3241 tcaacggcga agccggtgca tgggcacgca tcatgagcgg taccggctct gccagtggtg
     3301 gtttcagtga caactacacg cacgttcagg tcggggtcga caaaaaacac gagctggacg
     3361 gactggattt gtttaccggt ttcactgtca cacacactga cagcagtgcc tccgccgatg
     3421 ttttcagtgg taaaacgaag tctgtggggg ctggcctgta tgcttccgcc atgtttgatt
     3481 ccggtgccta tatcgacctg attggcaagt atgttcacca tgataatgag tacactgcaa
     3541 cctttgccgg actcggaacc cgtgattaca gcacgcattc atggtatgcc ggtgcagaag
     3601 cgggctaccg ctatcatgtc actgaggatg cctggattga gccacaggct gagctggttt
     3661 acggttctgt atccggtaaa cagtttgcat ggaaggacca gggaatgcat ctgtccatga
     3721 aggacaagga ctacaatccg ctgattggcc gaacgggtgt ggatgtgggt aaatccttct
     3781 ctggtaagga ctggaaagtg acagcccgtg ccggtctggg ctaccagttc gacctgctgg
     3841 ctaacggcga aaccgtattg cgggatgcat ctggtgaaaa acgcatcaaa ggtgaaaagg
     3901 acagccgtat gctgatgtcc gttggcctga atgcagaaat cagggataac gtccgctttg
     3961 gactggagtt tgagaaatcc gcctttggta agtacaacgt tgataatgct gtcaacgcta
     4021 atttccgtta ctcgttctga tactccgtat tcaaaagggc ctgcgggccc tttacgctca
     4081 gtgcctgccg ccctgatatg tctgagagac atttccggga tgtcagtcga ctggtttact
     4141 taactgcgat gactaaagct ggcagtaatg ccggcttttt tacggccaca tacgtcttcc
     4201 tctcatccgg aactgtgata caaatcctgc atacaggatt gaccgccggt tcctgcaaat
     4261 ggcacatttt cagtaagata gcgaaggtta catttgtcac tggttaacat accgatggta
     4321 aaaggggcaa gcagcgccag caattcatca gcccgggggc caaaagtgta ggccagaacg
     4381 ccaccagttt tcgtgttgta cgcgtaccag agcctgtgct gtcacgcttt actgccgata
     4441 aaactccatt gctcatcaag ttcgcagatg agtgccatat cagcatgagc gaccggggat
     4501 gacgttattc ttcgtggcga gcgtttttta aagtccgaat gacagtatta atgccgattt
     4561 tcagcgtacg ggcagtatcg cgaactccgg caccgttgaa aggcatttca gtgatttggt
     4621 cttgaactcc cggtttacgg gcctcgtaac ggtaagtgag ctgaaacact cgatgacagt
     4681 cacggcagcg aaatcgatca tgtccttttg ggttcttccc gtgacggtaa acctgaacgg
     4741 actggcaacg ggggcaatga aggttaactg tgaccattaa agaaccttaa aagtgactat
     4801 tataccccta attcaactaa ttggaggcat taccatgcat tagtgcaagc gccttgtgga
     4861 ttcttttgct aatggcggaa acatattgta caatattgca tttatccctc aataaataaa
     4921 tgagttatca gattgcaaaa atctgttatc aaactaattc aaataaaaag agattgatac
     4981 gaccaacgta cacaaaaatt ttcgatattc gaactaaata acagctccta ataccgctac
     5041 actttagcta tccagtctgc gcctcagaaa tggacgatgc cgttgaagga ctggtgtatg
     5101 gcaatgagcc gctttattat cgaattcggt gaccgcttgg acggtcactt cggagaaaag
     5161 gcatttacac agcatcctaa acaggttcag cataggtgat ata
//
LOCUS       DABSYT020000187         5110 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.188, whole genome shotgun sequence.
ACCESSION   DABSYT020000187 DABSYT020000000
VERSION     DABSYT020000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.188"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(11..229)
                     /gene="ccdA"
                     /locus_tag="ID725_RS23885"
                     /old_locus_tag="pgaptmp_004777"
     CDS             complement(11..229)
                     /gene="ccdA"
                     /locus_tag="ID725_RS23885"
                     /old_locus_tag="pgaptmp_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HGV7853206.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            772..1371
                     /locus_tag="ID725_RS23890"
                     /old_locus_tag="pgaptmp_004778"
     CDS             772..1371
                     /locus_tag="ID725_RS23890"
                     /old_locus_tag="pgaptmp_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGV7853207.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            1368..1988
                     /locus_tag="ID725_RS23895"
                     /old_locus_tag="pgaptmp_004779"
     CDS             1368..1988
                     /locus_tag="ID725_RS23895"
                     /old_locus_tag="pgaptmp_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853208.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            1985..2185
                     /locus_tag="ID725_RS23900"
                     /old_locus_tag="pgaptmp_004780"
     CDS             1985..2185
                     /locus_tag="ID725_RS23900"
                     /old_locus_tag="pgaptmp_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853209.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            2593..3570
                     /locus_tag="ID725_RS23905"
                     /old_locus_tag="pgaptmp_004781"
     CDS             2593..3570
                     /locus_tag="ID725_RS23905"
                     /old_locus_tag="pgaptmp_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGV7853210.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3855..4595)
                     /locus_tag="ID725_RS23910"
                     /old_locus_tag="pgaptmp_004782"
     CDS             complement(3855..4595)
                     /locus_tag="ID725_RS23910"
                     /old_locus_tag="pgaptmp_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7853211.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4716..4946)
                     /locus_tag="ID725_RS23915"
                     /old_locus_tag="pgaptmp_004783"
     CDS             complement(4716..4946)
                     /locus_tag="ID725_RS23915"
                     /old_locus_tag="pgaptmp_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HGV7853212.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
BASE COUNT         1280 a         1322 c         1330 g         1178 t
ORIGIN      
        1 aaactgcatt tcaccagtcc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc
       61 gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac
      121 gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca tatgccttga
      181 gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcacacctct
      241 ttttgacata cttcgggtat acatatcagt atatattctt atgccgcaaa aatcagcgcg
      301 caaatacgca tactattatc tggcttttag taagccttat gtattttacc tttcgttatg
      361 ttaaccaata aaaattaaaa tctgccttat aaaaacaaag cgtaattacc gcattcccgt
      421 ttcgtatgga tccgacttta tttgaataaa cgactttccg gaagtgtacc acctgagaaa
      481 atcatcatat cgctgttttc cgcccctgcg cggcgcatca cagtccagag atacaacaca
      541 acatatagtg ccatgggcaa ctgaatgagg cactgcatgc tgtgctgacg ggatgtaacg
      601 ctgtgcggag ggagaggaag atccgtaacc accagaaacc ggatctgacc aggaaaagag
      661 aaagagtgta tgtaagttct gtactgtatg tatgaattgt attgtatgta ttgtgtattt
      721 ttcataccgg gcatacgatg gcgccattcc ctgagaaacc ggagccagac catgacatcc
      781 ctctccccgg acaccgtccg ccgcattgaa gacgccgccg ccgccctgat cgccgccggc
      841 accccgaacc cgaccaacga gcaggtccgt cagcacctcg gcggcggctc cctgtcccac
      901 atctccccgg tgatgcgcgc gttccgcgcc cgccagcgcg agcaggccac gcccctgccg
      961 ccggagctgg cgcagctgct gaccggccag ctggggctgc tgtggcaggc ggccgtgaaa
     1021 caggctgaag ccggggccct ggcggcgcgc gagcaggccg acgacgacat tgcccgggcc
     1081 gacaaggagc gggatgaggc gctggcgaac gtggccgcgc tggaaagcga gctggcggtg
     1141 ctgcgcgagg tggtggccga acgtgaccgc ctgctgcagg aggtacgcga gctacgcgcg
     1201 gaggcgctgc cgctgcgcga gcaggtggcc cgcctgaccg ccaccggcga acacctggcg
     1261 gcgcagctgc aggacacgaa ggcggagctg aaggaagccc gggaggacgg ccgccagctt
     1321 cagaccgaac tgctggctct ggcccgccag gacgggaagg tgaagaaatg agacaggaaa
     1381 gagtattacg tataaaggca tttcgacggg cactggagct tggtggcggt gaacgggctg
     1441 acgggtgtcg tcattttccc cgctggaaaa cggaactgag gaatttcccg cacagcagct
     1501 gcgatttagc cagcaatact ctggcgtact acctcaaaga acgagagaga tgccatccgt
     1561 gcatcatttt tatggaaggg aacgctggct ttcatgaagc ggaaaactca acagttcacg
     1621 ggcatgtcat cgtattgctg gacggagagt acatcgattt gacgctggat caattcgacg
     1681 aatatccgga ctacattcct tacgaacctg tcgaatctga cgggcagatt ggtaagctgt
     1741 tgcgtaatat catgaagcat gaggacccgg taaaaacccg ccagattgat ctcgatggtg
     1801 gcgaagagct gtatgcctgg ttacgcgata ccgccgacga agtgctggca gcagatccgg
     1861 aatggcaggc ccgggaacgg tcgatagaag aagcaaggga agccgctatt acggtgttcc
     1921 cgtttctgtc tgacgtgcaa aaaactgagt gcgaacaggg cccggacagt caggaggctg
     1981 ccagatgaag atttatcatt acactgacct gaacggccta aagggcatca tcgaatcagg
     2041 ctcactgtgg gcgacgcact tttcctttct caatgacagt aatgagctga ctcacggcat
     2101 gaactgcctt gagaatgccc tgcagtacct gagggacgac tttaacccga aaacccttaa
     2161 gttcatcgaa caggcaccct tataagcggt tgtgtatgct gtttccctag aatgatttat
     2221 ccacatatcc ggaggctcga tccagtaaac agatccatga atgatcaaca aaggatccat
     2281 taaagatccc cataccgctg caaaccttgt cactcatggg ccgggaccac gatcacataa
     2341 gcagtggcat gttactgata aactgtaaca tgctaatgat aagctgtatt cagtaatcca
     2401 tatactgaag taagttaatg acataaacta tggtcagtac gccagactca gctgttaaat
     2461 acaggctgca ggtttttctt cagtcagtta gcggggctct gacacacgat ttgctgttta
     2521 ttcttttact gtccacaggc aggaggcttt ctggaaaacg aaaattcaga catcaaaaaa
     2581 ctgttcggcg aggtggataa gtcgtccggt gagctggtga cactgacacc aaacaataac
     2641 aacaccgtac aacctgtggc gctgatgcgt ctgggcgttt ttgtaccgac ccttaaatca
     2701 ctgaagaaca gtaaaaaaaa tacactgtca cgtactgatg ccacggaaga gctgacacgt
     2761 ctttccctgg cccgtgctga gggattcgat aaggttgaga tcaccggccc ccgcctggat
     2821 atggataacg atttcaagac ctgggtgggg atcattcatt cctttgcccg ccataacgta
     2881 attggtgaca aagttgaact gccttttgtc gagtttgcaa aactgtgtgg tataccttca
     2941 agccagtcat cacgcaggct gcgtgagcgc atcagccctt ccctgaaacg cattgccggt
     3001 accgtgatct ccttttcccg caccgatgag aagcacaccc gggaatacat cacccatctg
     3061 gtacagtcag cctactacga tactgaacgg gatattgttc agttacaggc tgatccccgc
     3121 ctttttgaac tgtaccagtt tgacagaaag gtccttctcc agcttaaggc gattaatgcc
     3181 ctgaagcgac gggagtccgc ccaggcactc tacaccttta tagagagcct gccccgggat
     3241 ccggcaccgg tatcgctggc gcggctgcgt gcacgcctca atctgaagtc tcctgtattt
     3301 tcccagaacc agacggtcag acgggcaatg gagcagctgc gcgagattgg atatcttgat
     3361 tacacggaga tccagcgggg gcggacaaaa ctcttctgca ttcactaccg gcgtccccgg
     3421 ttaaaagcac cgaatgatga gagtaaggaa aatccgttgc caccttcacc tgcggaaaaa
     3481 gtcagtccgg agatggcgga gaagcttgcc ctgcttgaga aactgggcat cacgctggat
     3541 gacctggaaa aactcttcaa atcccgctga acataaactg tagtcagtga agagtgttcc
     3601 tttactgact acagcttata ttatcaggtg cagtgagtgg tctgctcact gcagtttata
     3661 ttcagtttcc tgcagtgctg cctgtagctg agctgtcatc tgccggtccc ttacgtgagt
     3721 caccccgtaa cctgatgctg aggcattgct cccttcataa aacatgactt actcactaca
     3781 gcttatatac atgctccagc ttatgttatg tctgttctgc tgaccacagc ttatataagg
     3841 aagcgcgtat gccgtcacct caggggaggc agtgtacgca ggatctccgc agcatcccgc
     3901 ccgtcacctg tgaaaggcac tgccagcgtg gcagccatat ccagcgcaaa cactctggta
     3961 taaacctcca tcgaacgtgg atccctgtga cctgccaggg cctggatgac tttccggggc
     4021 tggcggtgat agagcatatg catgatatag ctgtgccgga aggtgtgtgg tgtgaccgga
     4081 atcgaaaagt gtactccgtc agcttcggcc cgtctgacag cctgcttcag ccagttgcgc
     4141 atggtttcgt cggtcacggc ccataatggt tcacgacgac ggggccgggt ggtgatcatc
     4201 cagctttcca tctgcctgac atagcttata tccgtcagcg gaaccaggcg gacttcatct
     4261 tttggcgggc gtccgcgtcg cgcacgcact ttttcggaca ggatccgcac aaacggcctt
     4321 gctccatcca ggtcaaatga ttccggcgtc agcatccggg cttcgccaat acgcattccg
     4381 gtattccaga gggtggcgaa cagcatatgg tgacgctgat ccggcatata gaaaagaagg
     4441 gcactcactt ccggggccag caggtaggcc ggtgtggccc ctgtggaagt ggccattctt
     4501 ctcagggaga gcgctgttgc aaaatccacc cccggcgcaa tgggtaacag gaagacgcgt
     4561 tctggtgaat tctgcagggg aatgacattg ttcatgagtt atttttcacg tagcacacgc
     4621 actgtttatg gatacaggta ttctgacctg aacgcccgga tccttccacg acaataaatc
     4681 gcagcgtgcg gatccttttg tttgcttatc acgcattatt gcgggtaaaa ccaaatacgt
     4741 aaaggtacgt tttgcagcag ggatcccgta ttaagccagt cgtggcgcgg gctgagggct
     4801 ggtttgccgt ttttgggaga attggggtaa tgccaggacg ctaatgatct atcaatggat
     4861 ggatgtaagt gacaattgaa aactgaaaaa tgcgctatgt agattctgac agatttatgc
     4921 acctgaaata cgttcaaaat ccacatagcg taactctgat tttccttacc agaaaaaaga
     4981 gggggaataa ccagtatcta tcccccctac acaattgcag aataataaat tataaaaata
     5041 agaactcagg atttatatac atgattacat gtaatattac tgagaaatat tttgagataa
     5101 aacctttttg
//
LOCUS       DABSYT020000188         5044 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.189, whole genome shotgun sequence.
ACCESSION   DABSYT020000188 DABSYT020000000
VERSION     DABSYT020000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.189"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..1025
                     /gene="tsuA"
                     /locus_tag="ID725_RS23920"
                     /old_locus_tag="pgaptmp_004784"
     CDS             <1..1025
                     /gene="tsuA"
                     /locus_tag="ID725_RS23920"
                     /old_locus_tag="pgaptmp_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGV7853213.1"
                     /translation="ALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSVGV
                     FALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALF
                     TYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVLLVITLWVVMKEL
                     KKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGIT
                     SPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRAADAQTTVRSGLG
                     GVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASWLVYVRPQRKARL
                     ATAAAN"
     gene            1039..1266
                     /gene="tsuB"
                     /locus_tag="ID725_RS23925"
                     /old_locus_tag="pgaptmp_004785"
     CDS             1039..1266
                     /gene="tsuB"
                     /locus_tag="ID725_RS23925"
                     /old_locus_tag="pgaptmp_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGV7853214.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1309..2733)
                     /gene="sbcB"
                     /locus_tag="ID725_RS23930"
                     /old_locus_tag="pgaptmp_004786"
     CDS             complement(1309..2733)
                     /gene="sbcB"
                     /locus_tag="ID725_RS23930"
                     /old_locus_tag="pgaptmp_004786"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGV7853215.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2915..4093
                     /locus_tag="ID725_RS23935"
                     /old_locus_tag="pgaptmp_004787"
     CDS             2915..4093
                     /locus_tag="ID725_RS23935"
                     /old_locus_tag="pgaptmp_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7853216.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     DQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            complement(4074..4265)
                     /locus_tag="ID725_RS23940"
                     /old_locus_tag="pgaptmp_004788"
     CDS             complement(4074..4265)
                     /locus_tag="ID725_RS23940"
                     /old_locus_tag="pgaptmp_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7853217.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(4343..4687)
                     /locus_tag="ID725_RS23945"
                     /old_locus_tag="pgaptmp_004789"
     CDS             complement(4343..4687)
                     /locus_tag="ID725_RS23945"
                     /old_locus_tag="pgaptmp_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853218.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHGFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
BASE COUNT         1239 a         1200 c         1271 g         1334 t
ORIGIN      
        1 gtgctctgct gggatttgtc atgcagcgtg ggcgtttttg cctgactggt ggttttcgcg
       61 atatgtatat cgcgaaaaat aatcgcatgt tttacgcctt gctgattgca atttcggtac
      121 aaagcgtggg ggtttttgcg ttaattcagg cgggcctact gacttacgaa gccggggcgt
      181 tcccgtggct tggtactgtt ataggtgggt atctcttcgg gctgggaatt gttctggcgg
      241 gtggatgtgc caccgggacc tggtatcgcg cgggtgaggg attgatcggc agttggatcg
      301 cgcttttcac ttatatggtg atgagtgcgg tgatgcgttc tccacacgcc agtggtttaa
      361 atcaaacctt gcagcactac actactgaac ataactctat tgctgagact tttaatttgt
      421 ctgtgtggcc gttggttgcc gttttgctgg tgataacgct ctgggtggtg atgaaagagt
      481 tgaagaagcc aaaactcaaa gtcgcgacct taccgccgcg ccgaaccggg atcgctcata
      541 ttctgtttga aaaacgctgg catccctttg tcacagctgt actcatcggt ttgattgcgc
      601 ttttagcctg gcccctgagt gaagcaaccg gacgtatgtt tgggttaggc atcacttccc
      661 caacggccaa tattctgcaa tttctggtcg cgggtgacgt gaaatacatt aactggggcg
      721 ttttcctggt gttagggatc ttcgtggggg catttattgc agccaaagcg agccgtgagt
      781 tccgcgttcg cgcagctgat gcacaaacaa cagtacgtag cgggctaggt ggtgtactga
      841 tgggcttcgg tgccagtatt gcaggtggtt gctctatcgg taatggactg gttatgactg
      901 caatgatgac ctggcagggc tgggttggcc ttgtatttat gattctcgga gtctggactg
      961 cgtcctggct tgtgtatgtt cgaccgcagc gtaaggcgcg actggcaact gctgcggcaa
     1021 attaagtaag gtgtaaagat ggcgattaaa aagctggatg tggtgacgca ggtctgtccg
     1081 tttccactca tagaagcaaa agcagcactg gcagaaatgg ccagcggcga tgagttggta
     1141 attgagtttg attgcaccca ggcaactgaa gcaatcccac agtgggcggc agaagaaggg
     1201 catgccatca ccgattatca gcagattggc gatgcagcgt ggagcatcac cgttcaaaaa
     1261 gcctgactca acattgtcct ccgccgtacc agcggcggag gcttcacatt agacaatctc
     1321 ttccgcgtac tgccaaagcg cttttaacag cgccactttt tctttatcgt cggcatactg
     1381 ctgcgccagc atctgaatct cttctgcata gccttgtaaa aactctggcg tgaagacctg
     1441 gcggcggtgc tccagccagc gttgctgctc ggcataatcc agcgtccccg ggaagttgcg
     1501 tgcccgataa ttgaacaaca gcttttcaat ccgtttatca acaaaagtga tatccagtgc
     1561 cggtaaatta cgcggctcgg tttcaagcac aattttcatt gctgcacgat cggcgtcact
     1621 gaaaaagcca ttataaagct gtgcatccac gttatctgaa ggcgtaaacg gttcggcttc
     1681 cgcgaatatc gccaccactt tttcacgcac ttgcggattt tcacgcagaa ttttcaggtt
     1741 atcgaggcaa tgctgacgat taattcccag tcggtcggca tcttccgggc gtagcgtatt
     1801 cgcctgggcc agcaccggac atttattgat atgtaccagt ttaaccggaa cggcggcgtt
     1861 atcgccaaga tcggtttttg cggtatataa acgctcgcgc aatgtgtcgc tatccagttc
     1921 cagtaatggc gaaatatctc ctgccaaatc caccataatt acggcattgc gattttcagg
     1981 atgccacgcc agcggtgcca cccagctggt attgccgcgc catgccccaa acattccgga
     2041 aacgtgaacc aggggtttca tctgcggaac atcaatcaac gccatcagtt tgtgtttatt
     2101 acgatgggta aagagataat caaacaggcg tggttgacgc gtttttacca gtttcgccat
     2161 cgcaatagtg gcgtacacat cagccatcgc atcgtgggcg ttgctatgtt caataccatt
     2221 cgctttggtt aaatgctcaa ggcgaaagct cggtagaccg tcatcatttt caggccagtt
     2281 tattccttcc gggcgcaggg catagcaggc acgcataaca tccagtaaat cccagcgcga
     2341 gttatcatgc tgccagctcc aggcgtaagg atcgtagaaa ttacgataaa aaacgttgcg
     2401 tgtgacttcg tcgtcgaaac gcacattgtt gtagcccaga atacaggtct tcggtacggt
     2461 aaaaagcgag tgaatacggg cggcaaatgc ggcttcgttt tctcctttcg cccgagcttc
     2521 ctgcggggta ataccggtaa ttaatactgc tccaggctgg ggtaaatagt catccgcggg
     2581 cttgcagtaa aagacttcgg gttcgccgat gacattgaat tcgtcatcgg tgcgaatggc
     2641 tgcgaactgt gcagggcgat ctaacgcggg gtgcgtgcca aaggtttcgt aatcgtgaaa
     2701 gaggaaggta ggttgcttat ctgtgtctgt cattgtctag cattgtccaa tgtagttaat
     2761 taactgtctg ttataactcg aaaaacaacc cttttttcac ctgagtatgt ctacgaacgt
     2821 caaatatagt tgtttactgt gcgctgttgt ccatgtgata ttgtgtacca gatgtgtacc
     2881 aaaccattat ttctgagcgt accaaatatt atttatggct ataagcgaca caaaactgcg
     2941 tactatttat ggtaaaccat attctggccc acaagaagtg gctgatgccg atggcctcag
     3001 cgtacgaatt tcaccgaagg gggtcatcca gttccagtac cgctatcgct ggcatggcaa
     3061 gcctaatcga cttgggcttg gtcgataccc atccctgtct ttgaaggatg ccagacagat
     3121 cactgctgac ttgcgaaagc tctatttctc aggaacggat ccacgcactt attttgaaga
     3181 gaaggtggag aactccatga cggtcgccca gtgtctcgac tactggttcg acaactacgt
     3241 ctctacaact ctcagagaaa agacccaagc actttaccga tcaacggtta tgaagcgcat
     3301 gcatgacgcc ttccctaatc gtccggcatc ttctatcacg gttaagcaat gggttgacct
     3361 gcttaccgaa gaagaaaaag ataatccacg ccgagcaagg caggtgctaa gtcaactaag
     3421 atcagcaatt agttggtgca tgcggcgtca gttgatagat agttgcgcaa ttatgagcat
     3481 ccaaccaagg gacttcggct cccgcgctga ggtaggggat cgggtactgt cgtatcacga
     3541 actggctaag atctggcttg ctattgaaag aagccgtgcg tctacgtcaa ataagctcct
     3601 tcatcagatg cttatgctgt ggggggcgag gctctcagag cttaggctgg caacaaagac
     3661 agaatttgac ctgctggaca acgtatggac cgtaccgaaa gagcatagca agatgggtaa
     3721 tgttatccgc cgtccaatct tcgatcaaat taagcctttt ctcgaaaagg ccatgacaac
     3781 gtacaatgat gttcttttcc ctggagaaga cataaacaaa ccgatcagca tcgctgcagc
     3841 taaccgattc gtaaatagaa taaggggagg gatggatcta ggttactggc gaacacatga
     3901 tttcagaaga acgcttgtta cacgtctgtc cgagatgaat gtcgagcctc atgttactga
     3961 gcgaatgctc ggtcatgaac ttggcgggat aatgtccgta tacaataaac acgactggat
     4021 agaggctcag cgcaaagcgt atgagcttca cgctgataaa ttgttctggc acatcaggag
     4081 catttctgat taacgccacc gttaagaatc cacccttcaa cagcttcacg aaggtatgat
     4141 ttggggtggg ttctgactgg cttcggaaat ccgtgccgtt tggtatagtt ccagattgtc
     4201 tgacgtgatg aaacaccgag cttgttcatc acttctttct caggaatcag gctggtatcg
     4261 gtcatcttaa ttctccaggc aaaaagaaac cgccatatag cggctctatc agatatgaac
     4321 aggcctcatc gagtgtgagg cgttagtcct tgcgtagctc gctgattctt ctgtaagtct
     4381 ctggtgcttt gtttccgtgt atcttcattt cagacttcaa cagagcaacg agggaatccc
     4441 attcgttgag gattcctttg aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc
     4501 tgatttctgg aatctgctca acaagtgcaa cgcatcgtct gaaatcggct gcgtcatgtg
     4561 gagcaccgaa gccatgacca tagatattct ttttcaggcc acatgcgatt gaggcaagag
     4621 ttgcgctact gatgccgaca tcgccagttg attgccattt caaaaccttc atagccaaat
     4681 ctgacatttc ttgtctccat aaaacaaaac tcgccgtagc gagttcagat aaaagaaatc
     4741 cccgcgagtg cgaggattgt tagttgcgct ctgctgctgc cttagccatt accatatcca
     4801 cacaatttca ccagacatga ttctcgcaat cactatcatc accaaagtga taatcacaac
     4861 tttaactggc ggcatcattc accatcctgc tgcggtgctg ctggatatac ttttctcatc
     4921 caatgcgtgg cgtcatgaat aacgaactga tattcatcat cagtaaacat cgagccatcc
     4981 cactgtagcc cgtcaagaac acctctggat ggagagaata cccagatatc gaaatcgtat
     5041 ttct
//
LOCUS       DABSYT020000189         4958 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.190, whole genome shotgun sequence.
ACCESSION   DABSYT020000189 DABSYT020000000
VERSION     DABSYT020000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.190"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..546
                     /locus_tag="ID725_RS23950"
                     /old_locus_tag="pgaptmp_004790"
     CDS             <1..546
                     /locus_tag="ID725_RS23950"
                     /old_locus_tag="pgaptmp_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGV7853219.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..4020
                     /locus_tag="ID725_RS23955"
                     /old_locus_tag="pgaptmp_004791"
     CDS             607..4020
                     /locus_tag="ID725_RS23955"
                     /old_locus_tag="pgaptmp_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGV7853220.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALRETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSSTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGRGSVTLEFKVFHKGNQRAGNITDCTVIVTKKAASGISIR"
     gene            4091..4690
                     /locus_tag="ID725_RS23960"
                     /old_locus_tag="pgaptmp_004792"
     CDS             4091..4690
                     /locus_tag="ID725_RS23960"
                     /old_locus_tag="pgaptmp_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGV7853221.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4750..>4958
                     /locus_tag="ID725_RS23965"
                     /old_locus_tag="pgaptmp_004793"
     CDS             4750..>4958
                     /locus_tag="ID725_RS23965"
                     /old_locus_tag="pgaptmp_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGV7853222.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPP"
BASE COUNT         1164 a         1202 c         1565 g         1027 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgc agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacaccggg ctattttcag ataaccgcca cgccgcatct tgcggtttat
     2821 gatccgacgg tacagtttga gttctggttc tcggaaacgc ggattgcgga tatcaggcag
     2881 gttgaaacca gcgcgcgtta tcttggtacg gcgctgtact ggatagccgc cagtatcaat
     2941 atcaaaccgg gccatgatta ttacttttat atccgcagtg tgaacaccgt tggcaaatcg
     3001 gcattcgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct ggattttttc
     3061 aaaggcaaga taaccgaatc tcatctcggc aaggagctgc tggaaaaagt cgatctgacg
     3121 gaggataacg ccagcagact ggatgagttt tcgaaagagt ggaaggacgc taacgataaa
     3181 tggaatgcca tgtggggcgt caaaattgag cagaccaaag acggcaaaca ttatgtcgcg
     3241 ggtattggcc tcagcatgga ggacacggag gaaggcaagc tgagccagtt tctggttgcc
     3301 gccaatcgta tcgcgtttat tgacccggca aacgggaatg aaacgccgat gtttgtggcg
     3361 cagggcaacc agatattcat gaacgacgtg ttcctgaagc gcctgacggc ccccaccatt
     3421 accagcggtg gaaatccacc ggtattttcc ctgacatcag acggaaagct gaccgctaaa
     3481 aatgcggata tcagtggcag tgtgaatgcg aactccggga cgctcaacaa cgtcacggta
     3541 aatgaaaact gtacgattaa gggcatgctg gaggcgactc aggtcagagg tgacttcgtt
     3601 aaagctgtat ccaaatcatt tccgaaacag gctggtacgt ggggtaacac ggaaacacca
     3661 aacgggacgg ttacagtcac catcagcgat gatcataact ttgaccgtca aatcattatt
     3721 ccgcccatta tctttaacgg aatagcgtat agcgatccgg gaagtggtaa taacccggga
     3781 ggtacaagat acacgggtta tggttttgaa gttcgcaaaa acggtgtatt aatcgcatcc
     3841 agagaaacta aaggggccat tcccggtagc tacagtgcgg ttattgatat gccgagtggc
     3901 aggggaagcg tcactctgga gtttaaggtt ttccataaag gcaatcagcg ggcaggtaat
     3961 atcaccgact gtacggtgat tgtgaccaaa aaagcggctt ccggcatcag tatccgttga
     4021 aatatttata accctaataa cgggcgccag aaatggcgcc ttttttattg cagaaaatcg
     4081 ggaggtaatt atgcgtaaag tttgtgcagc aattttgtcc gcagccattt gtctggccgt
     4141 atccggtgtg cctgcatggg cgtctgagca tcagtccacg ctgagcgcag gatatcttca
     4201 tgccagtacg gacgctcccg gcagcgatga tctgaacggg attaacgtga aataccgtta
     4261 tgagtttacg gacacactgg ggctgattac gtccttcagt tatgccaacg ccgaagatga
     4321 gcaaaaaacg cattacagcg atacccgctg gcatgaggat tacgtgcgta accgctggtt
     4381 cagcgtgatg gcagggccgt ctgtgcgtgt gaatgaatgg ttcagtgcgt atgcgatggc
     4441 gggtgtggct tacagccgtg tgtcgacttt ttccggggat tatttccgcg taactgacaa
     4501 caagaggaaa acgcacgacg tgctgaccgg aagtgatgac gctcgctaca gcaacacatc
     4561 tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa tcagtggccg ttgatgtcgc
     4621 ttatgaaggc tccggcagcg gtgactggcg cactgacggt ttcatcgtgg gtgtcggtta
     4681 taaattttga ttagtaacac agcgttatga cagcccgccg gttcaggcgg gcttttttgt
     4741 ggagtgggta tggcagcagt acaaatatca ggcgtgctga aagatggtgc gggaaaacca
     4801 atacagaact gcaccattca actgaaagcc agacgtaaca gcaccacggt ggtggtgaac
     4861 acggtggcct ctgaaaatcc ggatgaggca gggcgttaca gcatggacgt cgagtatggt
     4921 cagtacagcg tcactctgtt ggtggaggga ttcccgcc
//
LOCUS       DABSYT020000190         4908 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.191, whole genome shotgun sequence.
ACCESSION   DABSYT020000190 DABSYT020000000
VERSION     DABSYT020000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.191"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            270..812
                     /locus_tag="ID725_RS23970"
                     /old_locus_tag="pgaptmp_004794"
     CDS             270..812
                     /locus_tag="ID725_RS23970"
                     /old_locus_tag="pgaptmp_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGV7853223.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            912..2114
                     /locus_tag="ID725_RS23975"
                     /old_locus_tag="pgaptmp_004795"
     CDS             912..2114
                     /locus_tag="ID725_RS23975"
                     /old_locus_tag="pgaptmp_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGV7853224.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2334..3116
                     /locus_tag="ID725_RS23980"
                     /old_locus_tag="pgaptmp_004796"
     CDS             2334..3116
                     /locus_tag="ID725_RS23980"
                     /old_locus_tag="pgaptmp_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HGV7853225.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3131..4336
                     /gene="rhmD"
                     /locus_tag="ID725_RS23985"
                     /old_locus_tag="pgaptmp_004797"
     CDS             3131..4336
                     /gene="rhmD"
                     /locus_tag="ID725_RS23985"
                     /old_locus_tag="pgaptmp_004797"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HGV7853226.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQLRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4393..>4908
                     /locus_tag="ID725_RS23990"
                     /old_locus_tag="pgaptmp_004798"
     CDS             4393..>4908
                     /locus_tag="ID725_RS23990"
                     /old_locus_tag="pgaptmp_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGV7853227.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGH"
BASE COUNT         1140 a         1188 c         1327 g         1253 t
ORIGIN      
        1 ccttataaat aaaaagaaac catgagaata cggtaactca gaaatatgtg aaagtgacag
       61 taatctcttt tgcgatgata actgcactta ttattatttt tgtgtgtatc ggtaaactgt
      121 gtttattttt tcggcgttta attatttaag aagtatttag ttacaataag tgactagatc
      181 ccttttttcg ctcattgaat cctctgcgcc aggcagtaca atcccgcagc tttgggaatt
      241 ttgattaatt gcttgtggga aaggggataa tgaaaaaaat tgcgctagca ggtctggccg
      301 gaatgctgtt ggtttctgca tcggtcaatg caatgagcat cagcggccag gcgggtaaag
      361 aatacaccaa tattggtgtc ggttttggga ctgaatcgac gggcctggct ttaagcggta
      421 actggacaca taacgacgac gacggtgacg tcgcgggcgt ggggctgggg ttgaatctgc
      481 ctctcgggcc gttaatggcg accgttggcg gaaaaggcgt ttacaccaac ccgaattacg
      541 gcgatgaagg ctatgccgca gcggtaggag gtggtttgca gtggaaaatt ggcaacagct
      601 tccgtttgtt tggcgagtat tactactctc cggattcact ctccagcggt attaaaagtt
      661 atgaggaagc gaatgctggc gcgcgttaca ccattatgcg tccagtcagt attgaggcgg
      721 gttatcgcta cctgaatctg tcgggtaaag acggtaaccg cgacaacgcc gtggctgacg
      781 gcccgtatgt tggggttaac gccagtttct gatcccttcc ggcgcggcat tctgtcgcgc
      841 cattcagttt cttactacgc cttatctccc tttgcgttat agtgattcga taacaaaagc
      901 aggagataac catgttaaaa gtggaaatgc tatccaccgg ggatgaagtg ttacacgggc
      961 aaatcgttga cactaacgct gcctggctgg ccgatttttt ctttcatcag gggttgccat
     1021 tatctcgccg caatacggtg ggggataacc ttgatgactt agtcaccatt cttcgcgaac
     1081 gtagtcagca cgccgatgtg ctgatcgtta acggcgggct gggaccgacc agcgatgatt
     1141 taagcgcact cgccgctgcg acagcaaaag gtgaaggcct ggtgctgcat gaagcctggc
     1201 tcaaagagat ggaacgctat ttccacgaac gtggacgagt aatggcaccg agcaaccgta
     1261 aacaagcgga gctgcctgcc agtgctgaat ttatcaataa cccggtaggc accgcctgtg
     1321 gttttgccgt gcagcttaat cgttgcctga tgttctttac tcccggcgta ccgtcagaat
     1381 ttaaggtgat ggtcgagcac gaaatcctgc cgcgtctgcg cgagcgtttt tctttaccac
     1441 agccgccggt ttgtctgcgt ttaactacct ttggtcgttc ggaaagcgat ctggcacaaa
     1501 gcctggacac tctacaactg ccgccgggcg taacaatggg ctatcgctcc tcaatgccga
     1561 tcatcgaact gaaactcacc ggaccggcaa gcgagcaaca ggcgatggaa aaactgtggc
     1621 tggacgttaa acgtgttgcc ggacagagcg tgattttcga aggcaccgaa ggactgcccg
     1681 cgcagatcag tcgcgaattg caaaaccgcc agttcagcct gacgttgagc gagcaattca
     1741 ccggtggttt attggctttg caactttctc gcgcaggtgc tccactgctg gcgtgtgaag
     1801 tggttccttc acaggaagaa accctggcgc aaactgcgca ctggattaca gaacggcgag
     1861 ccaaccattt tgccgggctg gcactggctg tttcgggttt cgagaacgag catctcaact
     1921 ttgcgctagc cacgccagat ggcactttag ctctgcgtgt gcgtttcagc actacacgct
     1981 atagcctggc tatccgtcag gaagtgtgcg caatgatggc actgaatatg ctacgccgtt
     2041 ggttaaacgg ccaggacatt gccagtgagc atggctggat tgaggttgtt gagtccatga
     2101 ccttatccgt ctgattccgc cactctgtgc cggtttactg caccggcaca ttccccctcc
     2161 ggcaaaactt gtccaacctg ttgtactcaa taagaaaaaa tccgtgcgct gattcacaaa
     2221 tttgtgcgta atttctccag tccgttggac gaggagcggg atttgatgcg cttcacaatt
     2281 ttactccccc tgagcaacga gactgattcc agtatctaag actggagtcc agtatgttgg
     2341 aatcaagcaa agttcccgcc ctgacacggg ctattgatat tctcaatttg attgcccgca
     2401 ttggcccctg taatgctgcg acgatcattg acacattggg gatccctaaa agcacggcct
     2461 atttgctgct taatgaactc aggcgtcagc gttttcttag ccttgatcac caggaaaatt
     2521 tttgtttgtg gaccaagctg gtggagcttt ccggtcatgc gttaagcaag atggatctcc
     2581 gggaactggc tcgaccgcgt ttgacgcaac tgatggatac caccgggctg ttatgtcacc
     2641 tggggattat tgataacggc agcgcttatt acattttgaa agtggagtca tcagccacta
     2701 tcagcgtgcg ttcacatgaa ggaaaaagcc tttcgcttta tcgctccgga ataggtaaat
     2761 gcctgcttgc ctggcaacct gcaactgtac agcaaagtat tatcgaaggg ctggtctggg
     2821 agcaggcgac acccacaacg attactcatc cacagcaact tcacgaagaa ctggcgcgta
     2881 ttcgccgtca gggctggagc tatgacaacg gcgaagatta cgccgatgtc cgttgtgtcg
     2941 ctgcgccggt gtttaacgcc aataacgagc ttaccgctgc gatctcggtg gtcggtaccc
     3001 gcttacaaat caatgaagaa taccgtgatt atctggcggg caaggccatt gcctgcgcca
     3061 gggatatttc acgtctgttg ggatggaaaa gtccctttga cttacaagcc tcataacaat
     3121 ggagaacatc atgaccctac caaaaattaa acaggttcgc gcctggttta ctggcggtgc
     3181 gacagcagaa aaaggcgctg gcggcggtga ttatcacgac cagggggcga atcactggat
     3241 agacgatcat attgccaccc cgatgagtaa ataccgcgat tacgagcagt tacgccagtc
     3301 atttggcatt aacgttcttg gcacgttggt tgttgaagtc gaagcagaaa acggccagac
     3361 cggattcgct gtttcgacag ccggtgaaat gggctgtttt atcgtcgaaa aacaccttaa
     3421 ccgtttcatt gagggtaaat gtgtcagtga tatcaaactg atccatgatc aaatgctcaa
     3481 tgccaccctg tattactccg gctctggtgg cctggtgatg aacacgattt cctgtgtcga
     3541 tctggctctg tgggatctgt tcggcaaagt ggtcgggctt ccggtttata aacttttagg
     3601 cggcgctgtt cgtgatgaga ttcagttcta cgccacaggt gcgcgtccgg atctggcaaa
     3661 agagatgggc tttatcggtg gcaaaatgcc gacgcactgg gggccacatg atggcgatgc
     3721 ggggatccgc aaagatgccg ctatggtcgc ggatatgcgt gaaaaatgcg gtgaggattt
     3781 ctggttaatg ctcgactgct ggatgagtca ggacgtgaac tatgcgacca aactggccca
     3841 cgcttgcgcg ccctataacc tgaaatggat cgaagagtgc ctgccgccac agcagtatga
     3901 aggttatcgc gaactgaaac gcaacgcgcc agtcggaatg atggtcacca gcggtgagca
     3961 ccacggcaca ctgcaatctt ttcgtacgct ttcagaaacc ggtatcgaca ttatgcagcc
     4021 ggatgttggc tggtgcggtg gcttaaccac gctggtggaa attgccgcaa tcgccaaatc
     4081 cagggggcaa ctggtggtgc cgcacggttc gtctgtttac tcccaccatg cggtgatcac
     4141 cttcactaat acgccattca gcgaattcct gatgactagt ccggattgtt caacgatgcg
     4201 tccgcagttt gacccgattc tgctcaatga gccggttccg gtgaatggtc gtattcataa
     4261 atcagtgctt gataaacccg gttttggcgt cgaactcaat cgtgactgca atttgaaacg
     4321 cccctacagc cactaatcac tccgggcgtt gctgtgacaa cggtaacgcc cacatattct
     4381 aaggagcttt ttatgagcac cgctttgctt gacgccgtgg tgaagaaaaa ccgtgtgcgt
     4441 ttaattccgt ttatgttggc gctgtatgtg ctggcgtttc tcgaccgttc gaatatcggt
     4501 tttgccaaac agacctacca gattgatacc gggttgagta atgaagctta tgcgctggga
     4561 gcaggcattt tctttgtggt atatgcgttt ctgggtgttc cggcgaatct tttgatgcgc
     4621 aaactggggg ccagaacctg gattggtacg acaacactgc tgtggggatt tctttcggca
     4681 gccatggcat gggccgatac tgaagcgaaa tttctgatag ttcgcactct gcttggtgct
     4741 gcggaggccg gatttttccc tggtatgatt tatctcacct cgcaatggtt tccgcagcgt
     4801 aatcgcgcca gcattatggg gctgttctat atgggcgcac cgctggcgtt aacactggga
     4861 tcaccgcttt ccggcgcgct gctggagatg catggattta tggggcat
//
LOCUS       DABSYT020000191         4874 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.192, whole genome shotgun sequence.
ACCESSION   DABSYT020000191 DABSYT020000000
VERSION     DABSYT020000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.192"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1937)
                     /gene="rhsD"
                     /locus_tag="ID725_RS23995"
                     /old_locus_tag="pgaptmp_004799"
     CDS             complement(<1..1937)
                     /gene="rhsD"
                     /locus_tag="ID725_RS23995"
                     /old_locus_tag="pgaptmp_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HGV7853228.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSAVWLTHDPAYPESLPAAPLVRYTYTEAGELLAVYD
                     RSNTQVRAFTYDAQHPGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYHYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSR
                     SGETVRYRYDDAYSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRRYDNRGRLISVKDAQGHETRYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAVSTTQGGLTRSMEYDL"
     gene            complement(2004..4112)
                     /locus_tag="ID725_RS24000"
                     /old_locus_tag="pgaptmp_004800"
     CDS             complement(2004..4112)
                     /locus_tag="ID725_RS24000"
                     /old_locus_tag="pgaptmp_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGV7853229.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          993 a         1481 c         1251 g         1149 t
ORIGIN      
        1 aggtcgtatt ccatactgcg cgtcagcccg ccctgcgtgg tgctgaccgc ctttccccac
       61 gcatcgtact gtgtctcgct ccggttgccg tccggggtga taacggcagt caggtcgcct
      121 gcggcgttgt actcataccg cgtttcatgg ccctgtgcgt ctttcaccga gattaaccgg
      181 ccacggttgt catagcggcg gtaaaggctg atgccttcct cgcggtggac cgccgtcatc
      241 tggccgaagc ggtcgtattc atagcgggtc tggtagcccg agcagtcggt gaacgccagc
      301 aactgaccgt agcggctcca ggtcatctgc cgggtgctgc ccgtcgcatc tgttgtcgtc
      361 gccggtaact cactgtacgc atcatcgtag cggtagcgta ctgtctcccc gctgcgcgat
      421 gtctccgata ccagcctgcc cggttcatca tatgcccggc ggctctccag cccgtccggg
      481 gacaccaccg ccgtcagctg gttcccgtcg ttatagtaaa atttcgtctc ccgcccgtcc
      541 ggtgtggtga tgtccgtgat atcgccggat accacattca gaccatactc tgtccgccgt
      601 cccgccgcgt ccgtctgcgc cgttagcctt cctgccgcgt catacccgct gtgcgtgacg
      661 ctgccgtccg ccagttcttt tttcaccacc cgcttcagcc cggccccgcc ttctgtatgc
      721 agcacctcac gccggttcag gctgtccgtg acggtgatgc ggtcctgctc atactggtag
      781 tggtaactca ggcctgccgg gttcagttgc tccaccaccc gccccgcatc gtcgtagcgg
      841 tagcgcatct ccggccttcc cgcataacgg tgccccacca tccggcccgg gtgctgcgcg
      901 tcatacgtga aagcgcgcac ctgcgtattg ctgcggtcat ataccgccag cagttcaccg
      961 gcttccgtat acgtgtaccg caccagtggc gcagcgggca ggctctccgg gtatgccggg
     1021 tcgtgcgtca gccacaccgc cgaaaggcgg atacctctgt cgggaccgta ttcggtaccg
     1081 ggcagtgtgt cggggaacgc tgagtctgaa agagggcggg gggtgtcagg ggaagataaa
     1141 gaagcggtgt gctgtttacg ggcctcttcc gcccgctgcg cctgcgtggt cagcaccaga
     1201 cggaactccc gcccggcacc gtccgtcacg ccggtgattt ccccggccag gtcaccggcg
     1261 gcctcacgcc tgtacgtcag cgtccgcccg aagcggtccg ccatcccggt aagcacccgg
     1321 tacggcggca gcggcgctgg cagtacgtcc tcagcacccg gcacccgctc tgaccacccc
     1381 agtatccacc acggcccctg tgcgctgttg gtcgccaggt aaagatgcgg gcttaaccgg
     1441 atatccggcg gcagcgcccc ccacagccgc gccagcgtgt ggccgtccgg ctgcgctgcc
     1501 ttaccaccgc gcaccagcca cattgactcg ctgcggctgt acaccgcctc ccccggcagc
     1561 agcggctcaa agtgaatgct ccgcccgccg ttgtcattga gtaccagtgc atcatcgcgt
     1621 agctgtaagc ggatatcaga aggcgctttc cagccggggc caaaaatccc caccggcgca
     1681 ggcgtccggg tccggtagct gctgtaggtg cgggagagaa tgaacggcag cgggccgggc
     1741 agcgcaaggt ccgtctcgcc gggcagcacc ttcgccccca gcagcggatt taccgggttg
     1801 cccgaagtca tcccgcccgg gcacaccgag cacgccacgc cagttggcgc gccaattctt
     1861 acacctgccg aaccctggac aatgggaccg ccatactgag tcatatctcc ctgacgcgcc
     1921 gctggttttc cgctcatgat tcaattcctt cttctgttta tttttaatgc ttgtttgtta
     1981 ctgaataccg aacaggtacg ggatcagtac tctctcatat ccggcgtatc ctgcttttca
     2041 tcaggggtct tcggtgggga tttcgaggct gcacagttcc agttaatcaa cagtgagtca
     2101 ccattaacct gctccttacc ctgcaaatga atttttgtgc cgttgagaaa aatttgtccg
     2161 tccttcgtca gcaccagcct tgccttacca cagcagagct ccagatgctc accggcggag
     2221 taaatcgcgg tctgcccggt atcatccttt ttcgttttac caacggtgaa ggtaacgtta
     2281 tttccgactt tgacgtcata acccagcccg accctcaacg atttatgcaa accgatctgc
     2341 gaggactgca tcagagccac cgaagtgttc ataatgccac ctaccgtcgt ctggtacgcc
     2401 acgcccacgg tcagcgcacg aacaagtcca atcgtttcca cctgaacaga acccacgtta
     2461 atgatctggt tactgcccac cgtctctatc tggttaacgc ccaccgtctg tatctgattg
     2521 ttggtaaccg caatcatctg attgttgcca atggtctcag catggttgtt tatcacatcc
     2581 gtggtgcggt tattcagcac ctcggtgttc atgttcttct gcgcgtggat gtagacctgt
     2641 tctttccctg tcgcatcgtc aaacttcagt tcattaaacc cgctgccctt atagtttttc
     2701 gaacgaatgg tcatctgcgt cttcgtcccc ggcaggctgc cgggtgtgcg gttttcctgg
     2761 tggtaggtac gccccataat aatcggctgg tccggatcgc cgttgaggaa gtccacaatc
     2821 acctcctgac ccacacgcgg tatcgccagg ttgccaaagc cggtgcctgc ccacgcctgt
     2881 gccacacgga tccagcatga actgtcctgg tttgacgggt tatagcggtc ccagttaaat
     2941 ttcacccgca cgcgaccatg ttcgtcgcag aagatttcct cgcctgccgg tcccgtcacg
     3001 acggcgctct gcgggccatc caccagcggt ttcagcaacg gctgtggtcg ccatgtccgg
     3061 tctgccggta ttaccgcaaa gtggttattc agcgtggtac ctgaacccct gcgtcccggc
     3121 accgcctgtg gctgctcgcc gtgcagttca cttgccacca cctgccattc ccggttcagg
     3181 ttcgcctgcg gatgccccgt cagcacaatt cgccgtcccg gccatatttc cggagaacgg
     3241 cttgttccgc gcgccacttc tgcgttgttg cgccagccat ccatctgcca gcgggcaaag
     3301 ttctgcccgt gggcaccctt gaaacgtccg gggtagtcat acacttcata ctgtgtgcgc
     3361 tggtaatcct ggtactggcc ttcctgatca aaacgccctg cccagccggg tcgtttaaag
     3421 gtgtagtcct tggtcaccac ggaagaaggg cggatttgtg cgctgtagcg gaactggctg
     3481 atgcataggg tgctcacctc ggtacgggtg ttcgggttcc aggggatctc aaaggactcc
     3541 ggcagataac gcacggtgtc gcacaggacc aggctctggt cgatactttt ttgcgcgtgc
     3601 tcctcataaa agaagatgcc ttcctccgcc gccatccggc acaggaaatc gtaatcagtt
     3661 tcgccgtact ggacacaaaa ctcacgggaa ggatgcggct cgctgaacag cggactccac
     3721 tcggtcacac cgttttcttt caggatcgta gcgagtatgc tttcgatgtc ctcattctgg
     3781 aagatacgga agttctggcg cagccctgtg cgccacagcg gcgggcacac cttcatgctg
     3841 tacagcatct ggtttttgtc gttctccccc agttcaaacc aggtcaccac acctttcacc
     3901 cggcgctgta cgtcatcgcc ctgccatacc gtcaggtagg ccattttgtc cagcacctgc
     3961 gcaaattcaa gggaaagaaa ctgctgactg accagagaaa ggtcgaggga aaaaagtgaa
     4021 gagagtgact ggttcagatg aaaggagacc accgcaaacg cgtccggtgg caggccgtcc
     4081 acttccagcg tgaaacgtaa tccggttgac atagcaacct ccgtgaaaat gaataataat
     4141 ccgcacatat tgcatgtaca acatgtcggg cggatgaaat aagtgagtcg acatctggcg
     4201 ttaccgggga atatcagaaa aatgtcatgg ttatgtgatg atttagtctg ggattttact
     4261 tcagagaaac gggtgtctgt ttagtcaaca aaataatcac aaacgcaacg gatgggttct
     4321 cgacgttatt cacgacaggc tcgtacatta cagcgagtaa taaaaaacct gatatgcaaa
     4381 gtcaaaccaa tattcataat gtctttttat tcacaacaat aaataattta ataacaatca
     4441 ttgaaatgaa tgatgatttg tataaggaat atacgctacc gccccaccct gataacatga
     4501 tctgcacatt gccatcttaa cgtcgttaaa cactggctta atagtggata tttcaaatta
     4561 ggaatttatt aaattcacct gatgaatatc ataagcaaat agcatagaaa ataatgacaa
     4621 ctcccccgag gggaccattt ccaaagcact gcaccaccac ctggtgcccg gatgatcttg
     4681 cttcagtggt aagagtacct gattcattgt tcccaaaatg gtgttttccc ctgcccctcc
     4741 aataatgtgc agctctcacg tgataatcta ttgattaggt tatattaaga atcaatattt
     4801 aaaatacatc cattttcttg aataaatcaa catgtttatt aatcaattgc tgttttagct
     4861 ctgactcatc aagc
//
LOCUS       DABSYT020000192         4775 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.193, whole genome shotgun sequence.
ACCESSION   DABSYT020000192 DABSYT020000000
VERSION     DABSYT020000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.193"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            18..581
                     /locus_tag="ID725_RS24005"
                     /old_locus_tag="pgaptmp_004801"
     CDS             18..581
                     /locus_tag="ID725_RS24005"
                     /old_locus_tag="pgaptmp_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7853230.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            663..3302
                     /locus_tag="ID725_RS24010"
                     /old_locus_tag="pgaptmp_004802"
     CDS             663..3302
                     /locus_tag="ID725_RS24010"
                     /old_locus_tag="pgaptmp_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGV7853231.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            3325..4083
                     /locus_tag="ID725_RS24015"
                     /old_locus_tag="pgaptmp_004803"
     CDS             3325..4083
                     /locus_tag="ID725_RS24015"
                     /old_locus_tag="pgaptmp_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGV7853232.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            4094..4594
                     /locus_tag="ID725_RS24020"
                     /old_locus_tag="pgaptmp_004804"
     CDS             4094..4594
                     /locus_tag="ID725_RS24020"
                     /old_locus_tag="pgaptmp_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7853233.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            4591..>4775
                     /locus_tag="ID725_RS24025"
                     /old_locus_tag="pgaptmp_004805"
     CDS             4591..>4775
                     /locus_tag="ID725_RS24025"
                     /old_locus_tag="pgaptmp_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7853234.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVP"
BASE COUNT         1205 a         1208 c         1282 g         1080 t
ORIGIN      
        1 tggtttaagg acaaataatg agtaagtttg taaaaacagc tattgctgca acaatggtaa
       61 tgggtgcgtt cgcttctact tcaacaatcg ccgctggcaa caatggtaca gcacgtttct
      121 acggcaccat tgaagattcc ccgtgctcta tcgttcctga tgatcacaaa ctggaagttg
      181 atatgggtga cattggttca gggatcctga aaaataacgg gacttctaca ccgaaagctt
      241 tccagatcca tctgcaagac tgtgtgtttg acacccagac aacgatgacc actaccttca
      301 ccggtaacgc gtcttctacc aacagcggca attactacac catttacaat accgatactg
      361 gtgcggcatt taacaatgtc agcctggcca ttggtgacgc tcagggaacc tcttataaaa
      421 gcggcgcggg tatcgaacag aaaatcgtaa acgatacggc gaccaacaaa ggcaaagcga
      481 agcagacgct ggactttaaa gcctggctgg tgggcgctgc tgatgcgcca gatttaggta
      541 attttgaagc caacacgacc ttccagatta cttatctcta agtgataccc ccatagcttt
      601 cgggctatgg ggtttttaaa tggaattcgc gcaaattata ataattacgg atggatacgt
      661 gtatgcctga ccattctctt ttccgactgc gggtacttcc ctgctgcgtt gcattggcaa
      721 tgtccgggag ttatgtcaat gcctgggctg aaaatgaaat tcagtttgat tcccgttttc
      781 tggagttaaa aggcgacaca aaaatcgatc tgaagcgatt ttccagccag ggttatgtcg
      841 aacctgggaa atacaattta caggttcaac taaataaaca gccgctgacg gaagaatacg
      901 atatttactg gtacgcctct gagaacgatg ccagtaaaac ctatgcctgc ctgacgcctg
      961 aactggtcgc gcagtttggc ttaaaagagg atgtggcaaa aaacctgcaa tggatccacg
     1021 acggcaaatg cctgaaaccc ggtcaactgg aaggcattga tattaaagct gacctgagtc
     1081 agtcagcgtt agtcatttca ttaccccagg cttaccttga atataccgac atcaactggg
     1141 atccgccttc acgctgggat gacggtatat ctggtttaat tgctgactac agtattaccg
     1201 cccagacacg acatgaagaa aatggcgggg atgacagcaa tgaaattagc ggtaacggga
     1261 cggttggggt gaacctcggc gcatggcgtc ttcgtgccga ctggcagact gattatttgc
     1321 atagtaaaag caatgatgac gatgttatca acggtgatga cacgcaaaaa aactgggagt
     1381 ggagccgcta ctacgcctgg cgagccttac cgtcgctaaa agccaaactt ggccttggcg
     1441 aagactacct gaattctgat attttcgacg gctttaacta cgtgggtggc agtatcagca
     1501 ccgacgatca aatgttgccg ccgaatctgc gcggctatgc gccggatatc tccggcgtgg
     1561 cgcacaccac cgcgaaagtg accgtcagcc agttgggccg cgtcatctac gaaacccagg
     1621 tcccggcggg gccgttccgc atccaggatc ttggcgattc ggtctccggt acgctgcata
     1681 tccgcattga agaacagaac ggtcaggtgc aggaatatga catcaacacc gcctcgatgc
     1741 cgttcctgac tcgccccggc caggtgcgct ataaactgat gatgggccgc ccgcaggagt
     1801 gggggcacca cgtggaaggc ggtttcttct ccggcggcga agcttcctgg gggattgcca
     1861 acggctggtc gctatacggc ggggcgctgg cagatgaaca ctatcagtcg gcggcgcttg
     1921 gcgtcggtcg cgacctgtct gtgtttggtg cggtggcctt tgatatcacc cactcgcata
     1981 cccgtctgga taaagagacc gcctacggga aaggttcact ggacggcaac tcgtttcgcc
     2041 tgagctattc caaagacttc gatgaactga acagccgcgt cacttttgcc ggataccgct
     2101 tctcggaaga gaacttcatg accatgagcg agtatctcga tgccagcgac agcgaaatgg
     2161 tgcgcaccgg caacgacaaa gagatgtaca ccgccaccta taaccagaac ttcagggatg
     2221 ccggtgtgtc tgtttatctc aactacaccc gccataccta ctgggatcgc gacgaacaga
     2281 ccaactacaa cgtcatgctc tcgcactact tcaacctggg cagtatccgc aacatgagca
     2341 tttccatgac cggataccgc tacgagtatg acaaccaggc cgataaaggt gtgtacatat
     2401 cgctcagtat gccgtggggt gacagcagca ccatcagcta taacggcaac tacggcagcg
     2461 gttcggacag cagccaggtg gggtatttca gccgtgtcga tgacgcaacc cattaccagt
     2521 tgaacgtagg caccagcgac aatcactcca gcgttgacgg ttattacagc cacgacggat
     2581 cgctggcgca ggtcgatctc agcgctaact accatgaagg gcagtacacc tcggcgggta
     2641 tttccttaca gggcggcgcg acgctcaccg cacaaggtgg cgcgctccac cgtacccaga
     2701 atatgggcgg tacgcgtctg ctgattgatg ccgacggtgt ggctggtgtt ccggtggaag
     2761 gaaatggcgc ggcggtttac accaatatgt tcggtaaggc agtggtggca gacgtcaaca
     2821 actactaccg caaccaggcg tatatcgacc taaacaacct gccggaaaac gccgaagcca
     2881 cccagtccgt ggtgcaggcc acgcttaccg aaggggccat tggctaccgt aagttctcgg
     2941 tgatcagcgg gcaaaaagcg atggcggtgc tgcgtctgca agatggcagt tatccgccgt
     3001 ttggcgcgga agtgaaaaac gacagcgcgc agaacgtcgg tctggttgac gatgacggca
     3061 acgtctacct cgcgggcgta aaacctggcg agcatatgat cgtttcatgg ggcggtgtgg
     3121 cccactgcga tattcatctg cctgacccgc tgccagccga tctgttcaat ggcctgttat
     3181 taccatgcca gcaaacaggg gcgatatctc cttcgatgcc tcatgaaatt aagccggtga
     3241 tccaggagca gacccagcag gtgatgccaa cggaagcgcc agtatcggta tcagccaatt
     3301 aataacgtga ttaaggaatg atccatgacg aatctatttt gctctgccag aacggccgca
     3361 acgacgctgg cgttactgat aactgccgcc agcctgtcag tacaggcctc tgtcacgccg
     3421 gaccgtacac gcctgatttt taacgaaagt gataaatcaa tcagcgtcac gctgcgcaat
     3481 aacgacccga aaatgccgta tctggcacaa agctggatgg aagatggaca gggtaacagg
     3541 attagctcgc cactgacggt cctgccgcca gtgcagcgca tcgattccat gatgaacggt
     3601 caggtgaagg tacagggcat gccggatatc aacaagctgc ctgccgaccg cgagagcgtg
     3661 ttctatttca acgtgcgtga gatcccgccg aaatcgaaca aacctaacac gctacagata
     3721 gcgctgcaga cgcgcattaa gttgttctgg cgaccaaagg cgctggaaaa cgtcagcatg
     3781 aaaaacccgt ggcagcataa ggtgacctta accctcagtg gtcaggagtt taccgtcaat
     3841 aacccgacgc cgtacttcgt ggttatcagt aacgccagtg cgcagaaaaa tggcaatccg
     3901 gcagcgggat tttcccccat ggtgatatcg ccaaaaacct ctgtgccgtt aaaagtcaaa
     3961 atggatagcg tcccggtgct gacctatgtg aatgattatg gtgcgcgttt gccgctgttc
     4021 ttccgctgta cgggaaatac ctgtcaggtg gacgaagagc aaaacagcaa aggccaggga
     4081 taggagcaat agcatgaaag gagcttattc ggcctgggcg tggttattgc tgtgtagcat
     4141 tgtgagcacg cctgtaatgg cggggagtaa atcggttgcg atggtcttgc gtgtgctcgt
     4201 tgatgccccg ccgccctgta cggtaacggg tgcgagcgtg gagtttggta atgtcttcat
     4261 ttccaagatc gatggggtaa gttacaaacg ccccattgac tactcgcttg tgtgcaacaa
     4321 tctggcaatg gacgacctgc gcttaaatat gcaggcaacc acaaccgtaa ttaacggaga
     4381 aacggtaatc gatacgggta ttgccggttt tggtatccgt attcagaaag taagcgatca
     4441 ctccattttg gatttaacgc ctggcgcatg gttaccgttt aacttttcat ctggcgcact
     4501 ggctctggaa gcggttcctg tcgtgcagag cggtgtatcg ctaacggcgg cggaatttag
     4561 cgccagtgcc acaattgtgg tggattatca atgaaacgga tctctctctt aatgttgtgg
     4621 agctttagct ttatggcgct ctcgaatgtc tcttttcatg gttatttagt gcagccaccc
     4681 aattgctcta ttagtaatgg gcaaaatata gagttgacgt ttcgggacgt gaatattgat
     4741 gacattaacg gtagcaatta cgaacaggtc gtgcc
//
LOCUS       DABSYT020000193         4536 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.194, whole genome shotgun sequence.
ACCESSION   DABSYT020000193 DABSYT020000000
VERSION     DABSYT020000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.194"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..614
                     /gene="nleB"
                     /locus_tag="ID725_RS24030"
                     /old_locus_tag="pgaptmp_004806"
     CDS             <1..614
                     /gene="nleB"
                     /locus_tag="ID725_RS24030"
                     /old_locus_tag="pgaptmp_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HGV7853235.1"
                     /translation="YDKKLSDIYLENIHKQESYPASERDNYLLGLLREELKNIPEGKD
                     SLIESYAEKREHTWFDFFRNLAMLKAGSLFTETGKTGCHNISPCSGCIYLDADMIITD
                     KLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDG
                     LGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTCSSW"
     gene            663..1337
                     /gene="nleE"
                     /locus_tag="ID725_RS24035"
                     /old_locus_tag="pgaptmp_004807"
     CDS             663..1337
                     /gene="nleE"
                     /locus_tag="ID725_RS24035"
                     /old_locus_tag="pgaptmp_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HGV7853236.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDSYMKYINN
                     KLSKIE"
     gene            1715..2692
                     /locus_tag="ID725_RS24040"
                     /old_locus_tag="pgaptmp_004808"
                     /pseudo
     CDS             1715..2692
                     /locus_tag="ID725_RS24040"
                     /old_locus_tag="pgaptmp_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3212..4513
                     /locus_tag="ID725_RS24045"
                     /old_locus_tag="pgaptmp_004809"
     CDS             3212..4513
                     /locus_tag="ID725_RS24045"
                     /old_locus_tag="pgaptmp_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HGV7853237.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEILEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENTFKNIEMAIADIKREYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLACRTIQQNTPQKNIDFINLYNE
                     IRKKYNNNPSGQQEYLNKLRELYATYQKISTPLKHMFNSFF"
BASE COUNT         1553 a          837 c          919 g         1227 t
ORIGIN      
        1 tgtatgataa aaaactatct gatatttacc ttgaaaatat ccataaacaa gaatcatacc
       61 ctgccagtga gagggataat tatctgttag gcttattaag agaagagtta aaaaatatcc
      121 cagaaggtaa ggactctttg attgagtcat atgcagaaaa aagagaacat acttggtttg
      181 attttttcag gaatttggcc atgttgaagg ctggaagttt gtttacagag acgggaaaaa
      241 ctggatgcca taacatatcg ccctgtagcg gatgtatata tcttgatgcc gacatgatta
      301 ttaccgataa attaggagtc ctgtatgctc ctgatggtat cgctgtgcat gtagattgta
      361 atgatgagat aaaaagtctt gaaaatggtg cgatagttgt caatcgtagt aatcatccag
      421 cattacttgc aggcctcgat attatgaaga gtaaagttga cgctcatcca tattatgatg
      481 gtctaggaaa gggtatcaag cggcatttta actattcatc gttacacgat tataatgctt
      541 tttgtgattt tattgaattt aagcatgaaa atattatacc gaataccagt atgtatacct
      601 gcagttcatg gtaagtcttc cggttgtggt tgaaaaatat cgttctttag gtatagattc
      661 agatgattaa tcctgttact aatactcagg gcgtgtcccc tataaatact aaatatgctg
      721 aacatgtggt gaaaaatatt tacccggaaa ttaaacatga ttactttaat gaatcaccca
      781 atatatatga taagaagtat atatccggta taaccagagg agtagctgaa ctaaaacagg
      841 aagaatttgt taacgagaaa gccagacggt tttcttatat gaagactatg tattctgtat
      901 gtccagaagc gtttgaacct atttccagaa atgaagccag tacaccggaa ggaagctggc
      961 taacagttat atccggaaaa cgcccaatgg ggcagttttc tgtagatagt ttatacaatc
     1021 ctgatttaca tgcattatgt gagcttccgg acatttgttg taagatcttc cctaaagaaa
     1081 ataatgattt tttatacata gttgttgtgt acagaaatga cagccctcta ggagaacaac
     1141 gggcaaatag atttatagaa ttatataata taaaaagaga tatcatgcag gaattaaatt
     1201 atgagttacc agagttaaag gcagtaaaat ctgaaatgat tatcgcacgt gaaatgggag
     1261 aaatctttag ctacatgcct ggggaaatag acagttatat gaaatacata aataataaac
     1321 tttctaaaat tgagtagaga agctcagcca cttgtgtagg ggggctaaaa ttaattctat
     1381 ttcattatat gagaacacca ataagaataa taatatttga tcacaactga agatgctttt
     1441 tacaatgaag aattattgtt aaaaaacgct ttgttgaatc aaccgaactg ttgctgagtt
     1501 gcaggatcac atcacgtatt ttcctgaaaa cgcaggctgc ttagtagcaa agcagaagtt
     1561 caaaatcacc aactaaatag ctgcgccgaa tagtagatca atgaaaggga actcagcccg
     1621 gattgtgcga tctgatcaat cgccaaaaat ccccccctaa ccggactgag cgatgccgat
     1681 catagcacca attccccgcg acgaatgacg cctgatgcaa aaagcaatct ataaaactca
     1741 cgataaaaat tatgcccgca ggctgaccgc catgctgatg ctgcaccgga gcgctcgcgt
     1801 cagtgacgtt gccagaacgc tctgctgcgc ccgttcctcc gtcaggcgct ggattaactg
     1861 gttcacgctg tcgagtgttg aaggtctgaa atcattaccc gcaggacgtt cccgccgctg
     1921 gccgtttgag catatctgca cattgtttcg tgaactgata aaacactctc ccggatattt
     1981 tggctatcag cgttcacgct ggagtacaga actgctggca ataaaaatca acgagataac
     2041 cgagtgccag ttacatgccg gaaccgttcg ccgctggttg ccattagtcg ggcttgtgtg
     2101 gcgcagggct gcgccaaccc tgcgaacccg taacccgcat aaagcagcaa ttcacaaagc
     2161 actggacgaa tgtagtgcgg agcatccggt cttttatgaa gatgaagtgt atatctatct
     2221 taatcccaaa accggtgcgg actggcaact tcgcagacag caaaaacatg tggtaacacc
     2281 ggggcagaat gaaaaatact atctggctgg tgctttacat agtggaacgg gcaaagtcag
     2341 ctatgtgggt ggcaacagca aaagctcagc cttatttatc agtctgttga aacatctgaa
     2401 attgacatac cgacgggata aacctatcac gctcatagtg gacaactaca tcatccataa
     2461 aagtcgggaa acacagcgct ggctgaaaga aaatccgaag ttcagagtga tttatcagcc
     2521 agtttactcg ccgtgggtga atcatgttga gcggttatag caggcgcttc acgatacgat
     2581 aacgcgcaat catcagtgcc gttcaatgtg gtaactgctt aaaaatgttc attattttat
     2641 ggaaaccgtg agcccattcc ccggtggtaa atatgggctg ttaaaagtgt agcggtattc
     2701 ggcgcagcta tttaggatgg tcaggtcgca gaagttctgt gccactacag tgcgtgtact
     2761 aaacaaaatg acgagggcac gaatgtcata atacgtgcgt attacctgac agtagctacc
     2821 aactgagggg gcttattcca aacctgattt attcaacaaa gctggactta tccttactct
     2881 gaattatttt ccagacatat gcatcatgta ctggctcgct tggttcagga aatacaggag
     2941 caaatctaca gctattagat ttattcctat ttgtatgcaa agtcgcattg cataatgcgg
     3001 agctatgtta cagatacgcc tgacgtgcgt agtaaaatta taaagcaaca tatatataca
     3061 aggagaaacc gagaaaaata aatgagtcgt atgttgggta gcatcgagat aaatattttt
     3121 tttatcacat tagcaccttg gttaagggtg ttaataataa tattctctca cttattaaaa
     3181 cataaataat tttttaagcg gaaatgtaat tatgagactg ccagagaaag ttctttttcc
     3241 tcctgtcact agtggcctgt cagggcagga aaaacaaaaa aaaccgaaga gcattaccgg
     3301 atttcaggaa aattatcaac gcaatatcag gccaatcaaa acagcatcag aagcccgact
     3361 acgcttcttt gataaaatgg tttcgaaaga aaactctctg gaagatgttg tttctttagg
     3421 tgaaatgatt cagaaggaaa tttatgggca tgaacaaaga acattttcac cagttcatca
     3481 tacaggtaac tggaaatcat cattgttaca caacgcgctc cttggtctgg caaatgttta
     3541 taatggctta cgggaaacag aataccctaa cactttcaac agagatggta taaaaagtac
     3601 taactctttt agagataact tattgacaaa aacaagaact cccagagata attttgagga
     3661 aggaataaaa catcctgaac atgcaacaat accatatgac aacgacaatg aaagtaataa
     3721 attgctaaaa gcaggaaaga tagctggtaa caataacgag ctgttgatgg aaataaaaaa
     3781 ggaatcccaa agcgaccatc aaatccccct gtcagataag ttcctgaaaa ggaaaaaacg
     3841 atctcctgta gctgaagata aagttcaaaa ctcgttaaca ccagaaaatt ttgttcagaa
     3901 aatttcactt agtgatgagc ttaaaacaaa atatgcaaat gaaattctag agataaaaag
     3961 aataatggga gaatacaatc ttttaccgga taaaaacagt cgtaatggtc taaaacttct
     4021 acagaagcaa gctgatttac taaaaataat catggaggat acctctgtta cagaaaatac
     4081 ctttaaaaac atagagatgg ctatagcaga tataaaaagg gagtactatt cgcatacagt
     4141 tgatattgag aaaaatattc atgccatatg ggttgcgggt tccccacctg aaagcatctc
     4201 ggactatatt aagacttttc tcaaaaccta caaagagttt acttactatc tttgggttga
     4261 tgaaaaagca tttggagccg cgaaatttac cagtgtctta aaacaaattg catttgattt
     4321 agcatgcaga actatacagc agaatactcc acaaaaaaat attgatttta ttaatctata
     4381 taatgaaata aggaagaaat acaacaacaa cccatcggga caacaagagt acttaaacaa
     4441 actcagagag ctttatgcta cttatcaaaa aatatccacc cctctgaaac acatgtttaa
     4501 ttcatttttt tagaaaacat gattaaactt caggat
//
LOCUS       DABSYT020000194         4501 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.195, whole genome shotgun sequence.
ACCESSION   DABSYT020000194 DABSYT020000000
VERSION     DABSYT020000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.195"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            903..998
                     /gene="dqlB"
                     /locus_tag="ID725_RS24050"
                     /old_locus_tag="pgaptmp_004810"
     CDS             903..998
                     /gene="dqlB"
                     /locus_tag="ID725_RS24050"
                     /old_locus_tag="pgaptmp_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HGV7853238.1"
                     /translation="MINLLIIVLRAVVAVANALIAILELIRQFID"
     gene            1514..2329
                     /locus_tag="ID725_RS24055"
                     /old_locus_tag="pgaptmp_004811"
     CDS             1514..2329
                     /locus_tag="ID725_RS24055"
                     /old_locus_tag="pgaptmp_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7853239.1"
                     /translation="MSHLPAKIFQISRSDAWNLTISPDAARNMRDLAAQIGAGMPKYL
                     IAPEVSLLLSYIDDLSIRMYFETLWNTGARLNEALALKPADFMLTPTRQYPQPVVILK
                     TLKQRIKEAERRPGRPTTRHPPLHPDPRYRKPPEPAVRLVPVLDAGYALRMREYLATW
                     KKRIKHQPVWDITSRQTPLNWINTAVARAGRDGVTFSVPVTPHTFRHSYAMHLTMSGV
                     PPRVLQSLLGHRYARSTEIYTRVFSLDVLTGSGLSFTCDPQLAKQLLGYPDVL"
     gene            2444..3103
                     /locus_tag="ID725_RS24060"
                     /old_locus_tag="pgaptmp_004812"
     CDS             2444..3103
                     /locus_tag="ID725_RS24060"
                     /old_locus_tag="pgaptmp_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="HGV7853240.1"
                     /translation="MGKIITVAGHKGGIGKSTVLCSLCVCVIIKGKTACFLETDSQGS
                     IKDFIEERKTNDRLSEIPYFECYTDIPAMARKLAARFDYVFVDTPGMKSPAFVKALSC
                     ADILFTFIEPGAGIEINTLGRLVFDIKTAQAGVNPSMKAWIVLNKCSTNPSDSEASEL
                     RRQLNDDPDWLPVPRQRIYMRTAHKKAYNGGMGVHEYDDKRGNKARGEIELLLKETGI
                     L"
     gene            3139..3351
                     /locus_tag="ID725_RS24065"
                     /old_locus_tag="pgaptmp_004813"
     CDS             3139..3351
                     /locus_tag="ID725_RS24065"
                     /old_locus_tag="pgaptmp_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853241.1"
                     /translation="MLTHFLFVPNISPWQGWLFLTVAVITIAAGCISIHENRMHFSWT
                     DAVMVLIYCGLAGFLAVCGMRAPGLI"
     gene            3808..4110
                     /locus_tag="ID725_RS24070"
                     /old_locus_tag="pgaptmp_004814"
     CDS             3808..4110
                     /locus_tag="ID725_RS24070"
                     /old_locus_tag="pgaptmp_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853242.1"
                     /translation="MAKLPKKIDQEIKPLDLRQFVSSAAQKPASASAVTRINLTMTDD
                     DLDTAQEFQNEYGASRAEVIRAALAALRMVSAEERRMLFDDIRKNSPKAGRPTVNK"
     gene            complement(4188..>4501)
                     /locus_tag="ID725_RS24075"
                     /old_locus_tag="pgaptmp_004815"
     CDS             complement(4188..>4501)
                     /locus_tag="ID725_RS24075"
                     /old_locus_tag="pgaptmp_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HGV7853243.1"
                     /translation="VTREELGMMGEQLAIKEAEPVPRHELEKAQGRDVNEQDGLILDA
                     IDRYQAKFEAEGLAFNRQETEAELLHHDFTREVAEDRLEQQFVQEKAEQQNQQQSEQE
                     R"
BASE COUNT         1232 a         1045 c         1003 g         1221 t
ORIGIN      
        1 ccctgaacaa agtgatctaa taatacaaat tacagacatg gttatgcggt cattattgaa
       61 taatttggat atctcatatc gatatgtagt aaataattta ttggctgctc gttagaagaa
      121 cagcattggt atgactctga tgctccttaa aatcccactt ttcagagtgg gatttgttta
      181 atgcccagaa cagggggaat ttccattcat gcccaaaaaa tattttgatg tcctctcgcc
      241 aattctcgcg ctcagtcctt cctgaaaaac ctcagaattc acgtagcgca tcgccgctac
      301 tgcatccatc aaaaaaaatg acagcttttt taatcatctt gtgttactca acttactgaa
      361 aaaatgggat ttatgaaaat gccgccgtcg gcatcaagca gttggccagc gcaaccggct
      421 gcatcgaccg caacaaaaaa actgaaagag aaagcccctc aaatgaggga gaaaaaaagg
      481 aagtgcagtc aacgagaggc gtaaaaaaac ccgactggtg aggtcgggtt ttaatacggt
      541 tccggttctg agttggtggc tctgaccgga ggaagtcact ctacacctaa cgtttacatt
      601 ttatgcactc agccgcagta ttgcaactaa ctgtggttaa ggcaccacat ttagttgtgt
      661 atcgtgggtt ttatatacct tataatcatg aagttagccg tgtggtgtgg cttcttcctc
      721 tgcccttccc gtttatgcag gtatgaacga ttttaaagtc ctggctgaac aaacaggtgg
      781 tgctgtgttt agtgctctgg caggaggaag tcactctgca cctaacaatg cgagagaacc
      841 gttgtgacag gcagtgtaag agcaagcaac ggtgatttcc tccagtttta ctggagaaac
      901 ggatgattaa tctgttaatc atcgttctca gggctgtggt cgccgttgca aacgcgctga
      961 ttgcaattct ggaactgatt cgccagttca tcgactgatg acagcggaaa cgtaactaag
     1021 ggcaggaagt gaaccgcttc ctgcccttta acgtactgtg atatttttca aaaaaactca
     1081 ccaatattct cctcggcaga aatgacataa ctcactgtaa aataacaaat catctaaacg
     1141 ccagctttgt attgagcgcg ttggccaacg cctttatcag ctgcgaccgc accagataat
     1201 ttgaataaga tttcccgcat cacctggaca tacaggttat tctgtccaga cctgattagt
     1261 tacaacatac actgaattca tttcctttat ctggtgctct gctccggcat acacgcccat
     1321 atcctgggta attcaggata attgccagat ttcgtcctgc cctgccgttc atatccgcct
     1381 ggacaaaaat gcattatcgg aaaaaatgtc caaaaatttt tttggtgatc ggaattttta
     1441 ttgtcggacg cacctctcct ccaggtcata attgctgaca catcccaaaa aaacaggtgc
     1501 ggagatttaa aaattgagtc acttacccgc caaaattttc cagatttcac gtagcgatgc
     1561 ctggaattta accatctctc ccgatgcagc ccgtaatatg cgggatctgg cggcgcagat
     1621 tggcgcagga atgccaaaat atctgatagc accggaagtc tcactgcttc tctcctacat
     1681 tgatgatctg agcatccgca tgtactttga gacactctgg aataccggag cacgactgaa
     1741 tgaagctctg gcgctgaaac ctgctgattt tatgctgact ccgacgcgcc agtacccgca
     1801 acccgtggtg atcctgaaaa cactcaagca acgtataaaa gaagcagaaa gaagaccagg
     1861 acgcccgacc actaggcatc caccattaca ccctgatccg cgatacagaa aaccaccgga
     1921 gcctgctgta aggctggttc ctgttctgga tgccggatac gcattgcgta tgcgggaata
     1981 cctggcgacc tggaaaaagc ggatcaaaca tcagccagtg tgggacataa catcgagaca
     2041 gacaccgctg aactggataa acaccgcagt ggcacgggca gggcgtgacg gcgtaacatt
     2101 ttcggttccg gtcacgcctc acacgtttcg ccacagttac gcaatgcacc tgacaatgag
     2161 tggcgttcct ccgcgcgtcc tgcaatccct gctgggacac cgttatgcaa gaagcaccga
     2221 gatttacacc cgcgtttttt cgctggacgt ccttacagga agtggcctgt cctttacctg
     2281 cgatccacag cttgcaaaac agcttcttgg ataccctgat gtgctttaat aatgagtaat
     2341 aatacacacc attaaactat tgccagcacg caattgtacg gttatacttt catcacataa
     2401 aattgtgtat taatacacac tatttagcca agaggatttt gtgatgggaa aaatcattac
     2461 agttgcaggg cataaaggcg gcataggaaa aagcactgtg ctttgttcct tatgtgtgtg
     2521 cgtcatcata aaagggaaaa cagcctgttt cctggaaaca gactcacagg gaagcatcaa
     2581 ggatttcatc gaggaacgta aaactaacga ccggctgtct gaaataccgt atttcgagtg
     2641 ctacacagat attccggcaa tggcacgaaa actggctgcc cgctttgatt atgtgtttgt
     2701 tgatacgcca ggaatgaaat ctccggcctt cgttaaggca ctgagttgtg ctgacattct
     2761 gttcacattc attgagccag gagcaggcat cgagatcaac acgctgggac ggctggtttt
     2821 cgacattaaa acagcacagg ccggagtcaa tccgtcaatg aaagcatgga ttgtcctgaa
     2881 taagtgctca accaatccgt cagacagcga agcgtcagaa ctgcgccgac aactcaatga
     2941 tgatccggac tggcttccgg ttccccgcca gcgtatctat atgcgtactg cacataaaaa
     3001 agcctataac ggcggtatgg gtgtgcatga atacgacgat aaacggggaa acaaagcccg
     3061 tggcgaaatt gagttactgt taaaagaaac gggcattctt tgatgtgtat tattacacac
     3121 taaatacgag ggagatgtat gctcacccat tttttgtttg tacccaatat cagcccctgg
     3181 cagggatggc tttttcttac tgtcgcagta ataaccattg ctgccggatg tatttccatc
     3241 cacgaaaaca gaatgcactt ctcatggaca gatgcggtta tggttctgat ttattgtggt
     3301 ctggcaggtt ttttagctgt ttgcggtatg cgggcgcctg ggcttatctg agtagcaata
     3361 gtgtgtattg ttacacaaca aataacagga aacataacat gctcagtctt tttctgttct
     3421 tatcactatt gatactcctg ataaggatat tctccgccag gctaataatt ccggctgtgt
     3481 gcgtatttct gcctgtgtca tcactctgga tgggatttac tatgctcaaa caacatgaat
     3541 ccccttgcct gaatagttca ggttgtgttt tatcaccatt tgctcagttc agcaccctgc
     3601 tcggatggct ttttattgct gtatcaatat taaccacatg tgcttgcgta tgttttcttc
     3661 gtaagaataa gaaatgctgg tcatggcatg atgctcctat gcttctgctt gcaggtggcc
     3721 tggcaatttg cggcacctgg atgctggaac ttctacagtt gtaaaaagtg tgtataatta
     3781 cacactttat agaaagcagg ataccttatg gcaaagctcc ccaagaaaat agatcaggaa
     3841 attaagcctc tggatttgcg ccagtttgtt agttctgcgg cacaaaaacc ggcatcggca
     3901 tccgcagtaa cgcgtataaa tctgacaatg acagatgatg atctcgatac agcgcaggag
     3961 ttccagaacg agtacggtgc aagccgtgct gaggttatca gggcagcact ggctgcgtta
     4021 cgtatggtat cagcagaaga acgccggatg ctgtttgatg atataaggaa aaacagtccg
     4081 aaggccggaa gaccaaccgt taataaatag tgcgcatcat tacacaccat taaaggtaat
     4141 aaactataca gatttaagcc cacgaattaa tttcgtgggc tttcttttta tcgttcctgt
     4201 tcagattgct gctgattttg ctgttctgct ttttcctgta cgaactgttg ttcaagacga
     4261 tcttctgcta cttcgcgagt gaaatcgtga tgtaacagtt cggcctcagt ctcctgacgg
     4321 ttaaatgcta atccctcagc ttcaaattta gcctgatagc gatcaatggc atccagtatc
     4381 aaaccatcct gctcattgac atcacgacct tgtgcttttt caagttcatg tcgtggaaca
     4441 ggttccgctt ctttaatggc aagttgctct cccatcatcc ctaattcttc tctggttaca
     4501 g
//
LOCUS       DABSYT020000195         4473 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.196, whole genome shotgun sequence.
ACCESSION   DABSYT020000195 DABSYT020000000
VERSION     DABSYT020000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.196"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..518)
                     /locus_tag="ID725_RS24080"
                     /old_locus_tag="pgaptmp_004816"
     CDS             complement(<1..518)
                     /locus_tag="ID725_RS24080"
                     /old_locus_tag="pgaptmp_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7853244.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCS"
     gene            1005..1439
                     /locus_tag="ID725_RS24085"
                     /old_locus_tag="pgaptmp_004817"
                     /pseudo
     CDS             1005..1439
                     /locus_tag="ID725_RS24085"
                     /old_locus_tag="pgaptmp_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1481..2224)
                     /locus_tag="ID725_RS24090"
                     /old_locus_tag="pgaptmp_004818"
     CDS             complement(1481..2224)
                     /locus_tag="ID725_RS24090"
                     /old_locus_tag="pgaptmp_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGV7853245.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLRDISDCREYPSYLKINFADFSLIKGLISWANHCAE
                     YIEIFDESIAFTCLSAFSSEKQFGVFLFGCLKSTGAKVKTIIHTDLSAPWRLKDISSR
                     LYLSESLLKRKLKEEGVSFSKIILDERMQMAEYLLSTRCYPISKVAKVCGYASVSYFT
                     YVFRRYFGVSPSQYSQRSSESKILTHQGI"
     gene            3048..3821
                     /locus_tag="ID725_RS24095"
                     /old_locus_tag="pgaptmp_004819"
     CDS             3048..3821
                     /locus_tag="ID725_RS24095"
                     /old_locus_tag="pgaptmp_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853246.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAAADDDDLLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASKAEVILATVRGIVRGVFIADKWLKSTREHFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(3879..4433)
                     /gene="pinE"
                     /locus_tag="ID725_RS24100"
                     /old_locus_tag="pgaptmp_004820"
     CDS             complement(3879..4433)
                     /gene="pinE"
                     /locus_tag="ID725_RS24100"
                     /old_locus_tag="pgaptmp_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HGV7853247.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
                     RQKVAIIYDVGVSTLYKKFPAGDK"
BASE COUNT         1361 a         1023 c          910 g         1179 t
ORIGIN      
        1 tcggagcaac gctgccgcac tttgcgcatt ttctccaacg caccacgttt ctcaatttta
       61 cgcagaacgt tcagcagctc tagcggttta atctctccca caggccttgt tccgacataa
      121 ggaaaaatgt cgttcttaaa cgcttccatg atgtctgatg catatcccgc cgaccattta
      181 gcagatttaa gttgatgcca ctctctggct atcttttcga aggcgttctc tgactctgtt
      241 tgcatagcta gcttttgctc ttttcgaacc tcactagggt tctttccttc tgccacaagt
      301 tttcgagctt catcacgacg ggaacgagca tcggcaaggg tgatcgttgg gtaaacacca
      361 agcgagatca ttttgggttt gccggcatag cgatagcgga accgccaact cttgcttcca
      421 ttaggttcta taagtaatga caaccctagc ccatctccaa atgtataggc tttagcttca
      481 ggcttagcgc ggcgaatctg catatcgttt aaaggcatgt gtataggaat ccagaccgaa
      541 caggaacata tacacaatcc tatacacatt cagtaccgga ttctactgga tggttacgga
      601 ctactatgga cttaaaagaa taaaaaatgt tataaatcag agattttatg gactaatacg
      661 gacgtttgag gaagttgaga tggtgccgat aataggagtc gaacctacga ccttcgcatt
      721 acgaattata agaatccgct tctaattcaa agcattaccc catcaacact gcgctcacac
      781 gtcccaccac atcaaaacat gtaaagcctt gcaagccatt gcgaggcctt atgtgtctca
      841 gttttgtccc accttgtatt acgacttgca tagccaatga agataaacgt gacgacaaac
      901 ggcgcagcag tcttcttttc tttcacgact tctccagcat gcataccttt ctgccatgac
      961 tgaagtgaat gcctgttatg agcaaggatt ggcccagatg aatcgccctg gactgttgcg
     1021 atcgtgaagc catagactgg gccgcaagca cgggaggtta cgataaagcg acagtgcagg
     1081 atgtcatgtt aggagcgata gaaaaacgct ttggcgataa agtaccggaa cagtccatcc
     1141 agtggctgac agataacggt tcagcgtata gagcgcatga aacacggcag ttcgccagag
     1201 agttgaatct ggagccctgc acaacggcaa tcagcagtcc tcagagtaat ggaattgcag
     1261 aaaggttcgt gaaaacgatg aaggaagact acatcgcgtt catgccgaaa ccgaatgtaa
     1321 gaacggcatt gcataatctt gcagtggcga tcgaacatta caatgaaaac catccgcaca
     1381 gtgcgttggg ttatcgctct ccgcgagaat atcgacgtca gcgggtaacg ttaacttaag
     1441 ataccccccc tgtctggaaa taagggggca agaacaatga tcagattccc tggtgagtaa
     1501 gaattttact ttctgaactc ctctgagagt attgacttgg agaaacacca aaataacgtc
     1561 taaatacata agtaaagtat gagacactgg cataaccaca gaccttagct actttactaa
     1621 taggataaca acgagtgctg agtaaatatt cagccatttg catcctctca tcaagtatga
     1681 tcttactgaa tgataccccc tcttctttca atttcctctt tagtaaactt tcgctgagat
     1741 ataatcttga tgatatatcc ttaagacgcc atggtgcaga taaatccgta tgaataatcg
     1801 ttttaacttt agcccctgtg cttttcaaac atccaaacag aaatactcca aattgttttt
     1861 cagaagaaaa tgcagagaga catgtaaaag caatagactc atcaaaaatt tctatgtatt
     1921 cagcacagtg attagcccaa ctaattaatc ctttaattaa actgaagtca gcaaagttta
     1981 tttttaaata agatggatat tccctacaat cagaaatatc ccttaagtta ttagctttca
     2041 aatagcgact aataaactca gcgccaaaat ccgccactat cactctttca ggatatgtca
     2101 ggaaaaaatc tctagagcta gagtcgacaa gtacagatga acctctctcc agaaacacac
     2161 tctcttttcc gaaataaaca tcaaaggact ccaaaatcaa tataactgaa catgtagttg
     2221 ccatatcatc cccccaattt aactgaaacc aggatgaagt atatgtatat aaagttcact
     2281 ttgccaaact tcagtataaa aaaccactaa aaatagggtg tgataaaaaa taccgtaaca
     2341 ataaagcaaa ggattataaa ttccgttaca gttacaaatg atactcaaga aacaattcct
     2401 taggaaaaac ttatttacag ccaataagta agacacttat atgatatcaa gttttcataa
     2461 aacataacta ggctaaatag ctgcgcctaa taccgctaca cttttgccag cccatgtttg
     2521 cctccgggta ttgacccctt ctctacgcaa cttcagttcc caccaccaac tttgcggcag
     2581 ctttgtagga tcaatgtcta aaagaataat ggtgaccgat aagaaaacga ctgaataact
     2641 gcagattttc gctcaaaacc ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac
     2701 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
     2761 ttaggaaaag caagacggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     2821 ggatttgaac ccccggtaga gttgccccta ctccggtttt cgagaccgac ccgatcatca
     2881 aacgaaacat aaaattagct cacattatga ggaaaggtat ctttttgcgc tatgtaaatt
     2941 caaagggtta gcctcatttt cccgatggct ttctcaacac tactagttgt gagcccttgc
     3001 aatgttcatt aatatacgtc tcacaaataa ttcacagata ttgcaaaatg gatattactg
     3061 agtttccttc tggagtaatt gaacaccttg gctggtatgt ataccgattg attgatccta
     3121 gggacggaag caccttctat gtagggaaag gcaaaggtaa ccgcgtattt gcccatatgc
     3181 gcggtgaagt ggcagcggct gatgatgacg acttactgag caacaagcta aagcaaatca
     3241 gagaaataag attagcggga cttgaagtta tccatgtcat ccatcgacat ggaatgactg
     3301 atgaaaagac ggcgtacgaa gttgaagcag cacttattga tgcctaccct gggttaacga
     3361 atatcatgaa tggtgctggc agcaatgaat tcggcgccgc gcatgtcaaa gagttgatag
     3421 caacatatca acccgaaacc ataacatttc atcataaagc attaatgatt tccgttaaca
     3481 gaagtgcaaa agattcagag ctttatgatg cggttcgatt tagctggcgc attaatgtct
     3541 cgcgcgccag taaagcagaa gtcattcttg ctactgtaag ggggatcgtt cgaggagttt
     3601 tcattgctga taaatggcta aaatcaacgc gtgaacattt tcctacgatg aaatactggg
     3661 acgaagaccc tgactttgag gcaacacaaa gctctcgcta tggttttgaa ggtcgagaag
     3721 ccccacctga aatagcaaac ctttatcttg ggaaaaaaat accagatgaa ttaagaaaaa
     3781 aaggagctat gtctccagtt cgttactcac ctaatttttg aatcttcaag tggtaagcat
     3841 aaaccgcagc acgatcttgc atacgacgtg ctacggtttc atttatctcc ggccggaaac
     3901 ttcttataca gtgtcgatac accaacatca tagatgatcg ctaccttctg gcgaggaatt
     3961 cctgctgcaa ttaatcgtcc ggcctgcgcc cattgttctg gtgtaagttt gggacgacgt
     4021 ccaccaattc gtccctgtgc gcgagcagct tccagtccag cttttgttcg ttcaacaatc
     4081 agttctcgtt ccatttcagc cagggccccc atcacatgaa agaaaaagcg ccccattggg
     4141 gtactggtat caatagcatc cgtcaggctg cgaaaattaa cgccacgttc gcgcaactcc
     4201 tcaaccagaa tgaccagatg ccgcatactg cgccccagcc gatccagttt ccagacaacc
     4261 agagtgtcac ctgccgataa tgtcctgagc agttttttca gtcccggcct ttcggacttt
     4321 gtaccgctta tcttgtcttc aaaaatcagc tcgcatcctg cacagttcag cgcattacgt
     4381 tgtagatctg tgttctggtc atttgttgac acacgtacat agccaataag catggtagat
     4441 ctccctgaca aaagcaggaa tgatgccatt tgc
//
LOCUS       DABSYT020000196         4346 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.197, whole genome shotgun sequence.
ACCESSION   DABSYT020000196 DABSYT020000000
VERSION     DABSYT020000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.197"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..112
                     /gene="grlA"
                     /locus_tag="ID725_RS24105"
                     /old_locus_tag="pgaptmp_004821"
     CDS             <1..112
                     /gene="grlA"
                     /locus_tag="ID725_RS24105"
                     /old_locus_tag="pgaptmp_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HGV7853248.1"
                     /translation="SPGTTGPKRKTYRVGNGIVGQSNIWNEMIMRRKKES"
     gene            complement(496..951)
                     /gene="cesD"
                     /locus_tag="ID725_RS24110"
                     /old_locus_tag="pgaptmp_004822"
     CDS             complement(496..951)
                     /gene="cesD"
                     /locus_tag="ID725_RS24110"
                     /old_locus_tag="pgaptmp_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HGV7853249.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(965..2503)
                     /gene="escC"
                     /locus_tag="ID725_RS24115"
                     /old_locus_tag="pgaptmp_004823"
     CDS             complement(965..2503)
                     /gene="escC"
                     /locus_tag="ID725_RS24115"
                     /old_locus_tag="pgaptmp_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HGV7853250.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKSEYFIITKSSPVRA
                     ILNDFAANYSIPVFISSSVNDDFSGEIKNEKPVKVLEKLSKLYHLTWYYDENILYIYK
                     TNEISRSIITPTYLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEVRGVPECIKYIT
                     SLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNG
                     SLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQRQI
                     EISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMIRV
                     NALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTP
                     RILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEATLS
                     AGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPIKS
                     ASSE"
     gene            complement(2503..2958)
                     /gene="sepD"
                     /locus_tag="ID725_RS24120"
                     /old_locus_tag="pgaptmp_004824"
     CDS             complement(2503..2958)
                     /gene="sepD"
                     /locus_tag="ID725_RS24120"
                     /old_locus_tag="pgaptmp_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HGV7853251.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPTHLFSDIGRIV"
     gene            complement(2964..3536)
                     /gene="escJ"
                     /locus_tag="ID725_RS24125"
                     /old_locus_tag="pgaptmp_004825"
     CDS             complement(2964..3536)
                     /gene="escJ"
                     /locus_tag="ID725_RS24125"
                     /old_locus_tag="pgaptmp_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HGV7853252.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(3539..3916)
                     /gene="sctI"
                     /locus_tag="ID725_RS24130"
                     /old_locus_tag="pgaptmp_004826"
     CDS             complement(3539..3916)
                     /gene="sctI"
                     /locus_tag="ID725_RS24130"
                     /old_locus_tag="pgaptmp_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HGV7853253.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(4000..4299)
                     /gene="espZ"
                     /locus_tag="ID725_RS24135"
                     /old_locus_tag="pgaptmp_004827"
                     /gene_synonym="sepZ"
     CDS             complement(4000..4299)
                     /gene="espZ"
                     /locus_tag="ID725_RS24135"
                     /old_locus_tag="pgaptmp_004827"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HGV7853254.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
BASE COUNT         1244 a          815 c          836 g         1451 t
ORIGIN      
        1 aagtcctgga acaaccggac caaaaagaaa aacctaccga gttggtaatg gtattgtggg
       61 acagtctaat atctggaacg aaatgatcat gaggcggaaa aaggagagtt agacgtaatt
      121 ttttgagtat attttataga agaataaaaa taaagctata tttaagggtt ttgttttcca
      181 gggtaagcct ttttctcggc taaagtttga aggtcatttc ctgacaaata tactctgagc
      241 tatagatgat tggaaagatc agatttctaa tgctaagtta agatatactg attgtttcat
      301 aaacggaaat gatattaaat acagcagaag atagttcagg gcataaactc ctggttaatg
      361 gaataacccc acacctgtag ataaatctac cagtggtcgg tgctcaactt tgggagcact
      421 tcactatagc agctttatta tttccgatag aaaatcccca gtcagcctgg ggattggtat
      481 agctgatggt agtttttact ctgtattacc taacaatttt ttaatatttt ctttatatgt
      541 agtgtatttt tctttttcat tacaccacat taaacaagcc ttaaaggctt tcttggccat
      601 tttcttattt ccgacgagta agtagctaat tccagaataa taagatgccc tgggatcttg
      661 cataactaaa gttgcacagc gtgcgaaaca cagttgtgca tcttcatgat ttgataaacg
      721 ctgatgacat aagcccagag ataaggtgta gtcataattc cagtgttcca gatatgtgag
      781 caaatggaat agatttcttg cggttataac atcaccagat tgatatgctg tataggcata
      841 agaatgaagg tcattgagat cttgttgtgt cagatttgtt aatgacgcta atgtgccacc
      901 atcctggtag aaatcataaa tatcctctag agagctaaat ttcctgctca taaagcctca
      961 tttattattc gctagatgca gattttatcg gggttgcttt aattaaaaag agtcgaacaa
     1021 cactatgttt ttgttttacc gtactggaga ataaactacc aataacagga atgtcgctta
     1081 acagcggtat accatcttta cttgaacttt ctttatcttg gataaatccg ccaagtaata
     1141 aactttctcc tgcacttaat gtcgcttctg ttgtcatttc tgaattttgt acttcaggta
     1201 aggtgctgct ggcatcctga gcattgcttt ggttagtcga ttgattgcca tcctgaatat
     1261 caagtaacaa acgaactcgt tcccgacgtt ttccggtaag gctattcgag ctgtcatcaa
     1321 gtatacgtgg tgtcactctc agcaatgttc cgcttgtaat actttccaga gacgccacct
     1381 tttctccgga gacttttgta taaaacgtta cgtttttatc aagaatcgcc tgcatattat
     1441 ttaaggttat aattgaaggc tgagagagta ttttagcttt cgagttctgt tgtaacgcat
     1501 tcaccctaat cataaaatta ctggcattac ttatcacaga agagctgata ttagcctgtg
     1561 cggtagagct attaaaggca atcgtacctt gtcctgcatt taacgtaccg gaccagttta
     1621 cgccgagctg ctgaagatcg tttgcatcaa catcaataat tgagacagaa atttcaattt
     1681 gcctttgttc aatatcaagc tcagaaatta attgctgata gatatccatc gttatttcgc
     1741 ggtcttttac aacaactgca ttaagacgtg gatcagcgga aatcgttaca ctgttatcaa
     1801 acaggttacc gcttcgctcg acggcacctt taccggtact gggaagtgaa ccattacttg
     1861 ccattgtctt cagaatggag acaacacctg ggacgacaac attttggtca cggtatttgt
     1921 atgttatatc tgtcgctgac gcatagttca gtttaaagac cttaacaaca tctttatttt
     1981 tggctttaga ctgtgcctct ttatcaagac tttctgataa agatgtaata tattttatac
     2041 attcaggaac accccgaact tcaatcgagt taaatgtcgt aatttttctc acattgcaag
     2101 agtttttgtt tactgagatt gtatcactca gatattttag cagtgagtca atatccagat
     2161 atgttggtgt aattatactt cttgatattt catttgtttt ataaatatac aagatgttct
     2221 catcataata ccatgtgaga tgatatagtt tggacagttt ttccagtact tttacaggtt
     2281 tttcattttt tatttcgcca gagaaatcat cattcacgga actactgatg aaaacaggaa
     2341 tgctgtagtt agcagcaaag tcattaagga ttgcacgtac tggagaactt ttagtaataa
     2401 taaaatattc gctcttgcct aatctttttt ccagagagga cggggcagct tgtgcactgc
     2461 agcaaaatag tgctgtaaaa ataaaaaaac ttattttttt cattacacaa ttcgtcctat
     2521 atcagaaaac aagtgtgttg gcggcatcat tgtatctgat gaagataatt gctctagttt
     2581 atcaaattgc ttatcgagca aagaggcgag agtatgttgc attattgctt ttgacatgtt
     2641 tgcgcgatga ttattatctt tatgaaaaat ttggcaaaga taccacttct tattagacag
     2701 gaaaataccc atttgtttat attcgttact aacggccagg atattgcaga gtctgataaa
     2761 tgtatctgtt ggaacctgac gaaaatcacc actaagctca atatatagaa cccagtagtt
     2821 tccaaaatcg tgcaagaaag catttttttg gtagatataa aaagtgagat gcgttggtga
     2881 accgttgaca tcctttacaa aatcaagggc cgttagccag tcacaatcat tctttgctat
     2941 gccattatta ttgttcatac atattacccg tcctgtcctg aggatgactt gataacaaca
     3001 ctaatatttt ctaatttgag atcgtcaaca ctattcttaa ctaggttttt tatctgaatg
     3061 accgatggtg ctaagttaac ctctggcgag ctgatgacta aaacggctgc tgaggagggt
     3121 tgactttcgt tattgttaac atttaatgaa acgctgcaat cgataacacc aggtatttta
     3181 cttagcaacc tttcgatgtc ttgttctttg agatagttga tttttgcatt ttcttgtgat
     3241 ggcgatgcca ctaactggct aggtgggaaa ataacctcga tatctgcaaa tttctttttc
     3301 gggaagccgt tattatttaa aatcgttatt gcgcgaacaa aatcctcttt ttccacactg
     3361 agtgtcatat ttccactttt gtccatttct ttggaaacat tgacatcatt ggatagcaac
     3421 agtgcttgca tctgattagc ttctttttct gttaatcctg tatacaattg ctctttacat
     3481 gccactgtca aacaaatagc agccaataaa aaaaggtttt taatgtgttt tttcattttt
     3541 attgcatcga aactaatttt gtaaccgatg ttgaaaggag gcttgctaac ttagatacga
     3601 catcaacgtt gacgtttgcg ttgagcacat tcttttgtaa ggtgagcacg tcttctggcg
     3661 agagcccacc tgcagataga gcatcgaaac tgagatcgct gttcattgtt gcaagatgtc
     3721 tctttttaat ttcttcaatc aacacttgtt ctggagaaga cactcccgct gttttaggga
     3781 gtaattgatc aagaaactga gccgcacctg ccgcagaggg ggctgaggct aacgaggtat
     3841 gctcatcaac gaaatttgtt tggggcgctt tatttaattg aatattaatg ttttccgatt
     3901 gattaatgtt gttcatattt actgttagcc tctttttatg aaagaggcaa gggcataata
     3961 catccattac ctctatcttt tatttatgcg atgggccggt taggcatatt tcatcgctaa
     4021 tgcaccgcca accgcagtaa gagtacctag tacgccagaa gtaataccca gggctaagta
     4081 ttctgtgctg ctcgtctccg agcatgccgc tgcaatacct gtacctagag ctgccagagc
     4141 cgtagtggta agtgctaatc cggctataac tctaacggtg cgatttgttc cattttctgg
     4201 tttaatcact cctgtcttct catccactga gttatttcca ctgagtgagg tcgccagcgg
     4261 cattactgca ccagaaggac ttaaatttgc tgcttccatt gatctttctc cttttgtcta
     4321 atttagacat ttacctggtt ataaat
//
LOCUS       DABSYT020000197         4338 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.198, whole genome shotgun sequence.
ACCESSION   DABSYT020000197 DABSYT020000000
VERSION     DABSYT020000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.198"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            33..566
                     /locus_tag="ID725_RS24140"
                     /old_locus_tag="pgaptmp_004828"
     CDS             33..566
                     /locus_tag="ID725_RS24140"
                     /old_locus_tag="pgaptmp_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7853255.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            616..1299
                     /locus_tag="ID725_RS24145"
                     /old_locus_tag="pgaptmp_004829"
     CDS             616..1299
                     /locus_tag="ID725_RS24145"
                     /old_locus_tag="pgaptmp_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGV7853256.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSHNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            1312..3762
                     /locus_tag="ID725_RS24150"
                     /old_locus_tag="pgaptmp_004830"
                     /pseudo
     CDS             1312..3762
                     /locus_tag="ID725_RS24150"
                     /old_locus_tag="pgaptmp_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3775..4338
                     /locus_tag="ID725_RS24155"
                     /old_locus_tag="pgaptmp_004831"
                     /pseudo
     CDS             3775..4338
                     /locus_tag="ID725_RS24155"
                     /old_locus_tag="pgaptmp_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT         1407 a          854 c          849 g         1228 t
ORIGIN      
        1 gaccttccat accagttaat taaggatgat ttatgaaaaa gtatattatt cccttcgttg
       61 cgacaacgtt gatgtttagc gcaacaacct tcgccaagga tgtcgatcaa ggaacactaa
      121 ccattagagg agaagttgtt gatacaacgt gttactttgt taatggtgat tcgacaacag
      181 atatcgttgt cgaagatgtt gtaaccagtg caatgaatca acttatcaat aatgaaatct
      241 atactttaaa aaccggttca acctctcatg acttagaaat tcagtgtgac ggaaatacag
      301 ccccaagtat cgaagtacta gcatcagagt ttgatgctaa taactttacg ctgaataaag
      361 gaactgctga gaacattggc tttgccattt tcctcgatga tgggactttg accagaatga
      421 gaccagggtt aaaaacaccg ctgaaagata atggctctgg tcaatatttt ctcaatttaa
      481 atgcacaata tgcccgcaca agtggcaatc ctgttacaaa aggctccgta gagtcaaccg
      541 taacgattaa aatcagtgca gattaacgtt ttttgccggt agctctgcta ccggtattat
      601 ttacggagtg attacgtgag aataataaca gcattactga tgtcattttt tattttacct
      661 gttcatgccg gtgtggtcat ctatggtacg cgcattattt atccggaaaa aaacagagaa
      721 gttctcgtac agttaatgaa tcaaagtaaa aatgcctctt tgattcaggc atggatcgat
      781 gatgggaaca ccactatcgc gccagaaaaa attcaggtca catttattct cacaccgcca
      841 gtaagtcgtg tagcaggtgg ttctggccaa caacttaaaa taagaaaaat gccaaacaat
      901 ttgcctcaca ataaagaaag tttattttat ctaaatgttc tggatatccc tccaaacaat
      961 ccacaaaatg cagggaaaaa caaaattaaa cttgcattgc aaaaccggat aaagttactt
     1021 tggcgtccaa gcggaatagc tccggtagat aaaaaaagcc tttctcaatt aaatatcaaa
     1081 aagaaaaata acgccatctc aataaacaac gaaacagcca actggataac ggttacaact
     1141 atcaaagcac aaaacgtgaa agttaataat gaaagtatct tactgcctcc cttttcgcat
     1201 aacgacatta cattaaaaaa taaccacgca tcagaatatg aactaacggt agttgatgat
     1261 tacgggaata acatccatag taaaatcgcc gcgagataac aggaaaaagt catgttaagg
     1321 acgacacgat gggtcgctgc tattattttt ttgtattctt ttccgggtta tgctgaagaa
     1381 acttttgata cgcactttat gatcggcggc atgaaaggag aaaaagtttc cgaatatcat
     1441 ttcgataata aacaaccgct tccaggaaac tacgaacttg atttttatgt aaataaccag
     1501 tggcgtggaa aacaagatat cacgatccca gagtcaccag tcaaaccatg tctgcctaag
     1561 gtgctactaa ccaaactggg ggtaaagacc ggtaacctca atacagaaga taattgtatt
     1621 ttactggata aagcggttca tggtggacaa tatcagtggg atatcagtga acaccgacta
     1681 aatttaacag taccacaggc gtatatcaat gaacttgaaa gaggttacgt tcccccggaa
     1741 agttgggatc gcggcattga tgctttttat acctcctata atttaagtca gtatcgctct
     1801 tatgacagca ataataacag taatacagcc agttatggcc gatttaacag tggattaaat
     1861 ttatttagtt ggcagttaca ctctgatgcc agctatagca aacccgatga tatgaaaggc
     1921 acatggcaaa gtaatactct ttacctggaa catggctggt cgcagatatt aagcacagtt
     1981 cagataggag aaaattacac atcctcactc atattcgact ctctgagatt tagcggaata
     2041 cgcttatttc gcgatatgca aatgctacct gattctatgc agagctttac acctttagta
     2101 caaggtgttg cgcaaagtaa tgccttgata acggtttcgc aaaatggcta catcatttac
     2161 caaaaagagg ttcccccggg accatttact atcgcagatt tgcaactctc gggcagcggc
     2221 tctgatctcg atgtcagcat aaaggaagca gatggcagtg ttcgttcctt tttggtgccc
     2281 tactcttccg tgcctaacat gctgcaacct ggtatttcaa atttcgattt tatcgccgga
     2341 cgcagtaaga tatatggtgt aaagaatcag gaggactttc tcgaggcaaa ttatatatat
     2401 ggcctcaaca atcttttgac actttatggc ggtacgattt tatccgataa ctataatgct
     2461 ataactctgg gaaatggttg gaatacaccg ctgggtgcca tctcttttga cgctacacga
     2521 tcgagtagta agttaaataa tgatataaca cacgaaggta cgagctatca ggtcgcctat
     2581 aataaatatc tagtacaaac ggccactcga tttagcgtgg ccgcctggcg ttatgcttcg
     2641 caggattaca ggacattcag cgaccatctt tacgaaaatg ataaaataaa tcatcagagt
     2701 gactatgacg atttttatga tattggcaga aaaaatagcc tttctgccaa tatcatgcaa
     2761 cctttatcca ataatctggg aaatgtatca ttaagtgcgc tttggcggaa ttactggggg
     2821 cgaagtggaa atgctaaaga ttaccaattc agttattcca atagctggca acgcatcagt
     2881 tatactttct ctgccagcca atcctatgat gaaaacgaca aagaagagga gcgttttaat
     2941 ctgtttatct ccattccttt ctactggggg gatgatattg ccaaaacacg tcaccaaatt
     3001 aacttatcga attcgacctc attttccaaa gatggctatt cctccaacaa tactggaatt
     3061 actggcatag ccggtgaaca tgatcagtta aattatggta tatatgttaa tcagcaacaa
     3121 caaaataatg atacatcgct tggtacgaat ttaagctgga gaactcccat cgccacaata
     3181 gatggcagct atagccattc taaaaacgcc tagcaaagtg gtggaagtat tagtagtgga
     3241 ttagttgtct ggcccggtgg tattaatatc actaaccagc tatccgatac atttgcaatt
     3301 ctggatgcgc ctgggttaga aggcgcgcat attaatggac aaaaatacaa ccgaacaaac
     3361 agcaaaggcc aggttgttta cgacctgatg atacctcatc gtgaaaacca tctggtactt
     3421 gatacagcaa acagtgaaag tgaaacagaa ttgcagggca atcgtcaaat tattgcgcct
     3481 taccgtggag cagtttctta tgtgcagttt acaactgacc aacgtaagcc ctggtatata
     3541 caggcactgc gtcccgatgg ttcgccatta acttttggct atgacgtact ggatctccag
     3601 gaaaacaata ttggagtcgt tggccagggt agtcgccttt ttattcgcgt agatgaaatt
     3661 ccaactggca taaaagttgc tctcaatgat gaacagaatt tattctgtac tattactttt
     3721 caacacgtta tcgatgaaaa caaaacatat atatgccagt aaaaggagtt tactatgaag
     3781 tggcttttat taatcacatt gagtctatat tcttttattg tgcaaagtgc tccctgtgga
     3841 ttgaccaatg tcggggaaca acgtggaaca tatatactca aaccactgag tatgaaaggt
     3901 aatctcaccg ccgaaaaaat tgaaaatact gggaatatca ttgagtgtgc agacttgcaa
     3961 gagacgacca acgccattaa agttcatttt ttaacacaga acgctttaag gtttaaagtc
     4021 ggaagtcgtc attatattat taatttcccc tttgaatctg acggtaaaaa aaatagcgct
     4081 tccagggaag ttatcttata atttagaaga aatgctatct gcgataaatt tcaccattcc
     4141 atactctatc gcatctgtat cagatagttc caacaccgca gatatttttc caggaactcc
     4201 ttttcctttg ttgttcgaaa ttgaagtgag ttcatgtaca gggaataata ctaattgtaa
     4261 tagcgtccgc tttaatttta atttaaatac tattttacag gtaaatttaa tgacctgtgg
     4321 ttttgaagat caatccat
//
LOCUS       DABSYT020000198         4289 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.199, whole genome shotgun sequence.
ACCESSION   DABSYT020000198 DABSYT020000000
VERSION     DABSYT020000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.199"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            42..503
                     /locus_tag="ID725_RS24160"
                     /old_locus_tag="pgaptmp_004832"
     CDS             42..503
                     /locus_tag="ID725_RS24160"
                     /old_locus_tag="pgaptmp_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGV7853257.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKVVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            549..758
                     /gene="hha"
                     /locus_tag="ID725_RS24165"
                     /old_locus_tag="pgaptmp_004833"
     CDS             549..758
                     /gene="hha"
                     /locus_tag="ID725_RS24165"
                     /old_locus_tag="pgaptmp_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGV7853258.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            796..1134
                     /locus_tag="ID725_RS24170"
                     /old_locus_tag="pgaptmp_004834"
     CDS             796..1134
                     /locus_tag="ID725_RS24170"
                     /old_locus_tag="pgaptmp_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853259.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            1374..1628
                     /locus_tag="ID725_RS24175"
                     /old_locus_tag="pgaptmp_004835"
     CDS             1374..1628
                     /locus_tag="ID725_RS24175"
                     /old_locus_tag="pgaptmp_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGV7853260.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            1864..1938
                     /gene="tap"
                     /locus_tag="ID725_RS24180"
                     /old_locus_tag="pgaptmp_004836"
     CDS             1864..1938
                     /gene="tap"
                     /locus_tag="ID725_RS24180"
                     /old_locus_tag="pgaptmp_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGV7853261.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            1931..2788
                     /gene="repA"
                     /locus_tag="ID725_RS24185"
                     /old_locus_tag="pgaptmp_004837"
     CDS             1931..2788
                     /gene="repA"
                     /locus_tag="ID725_RS24185"
                     /old_locus_tag="pgaptmp_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGV7853262.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3701..3985
                     /locus_tag="ID725_RS24190"
                     /old_locus_tag="pgaptmp_004838"
     CDS             3701..3985
                     /locus_tag="ID725_RS24190"
                     /old_locus_tag="pgaptmp_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HGV7853263.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            3985..4260
                     /locus_tag="ID725_RS24195"
                     /old_locus_tag="pgaptmp_004839"
     CDS             3985..4260
                     /locus_tag="ID725_RS24195"
                     /old_locus_tag="pgaptmp_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGV7853264.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
BASE COUNT         1156 a         1042 c         1085 g         1006 t
ORIGIN      
        1 gcctgaaata caggtgctat ttatctgata cggagaataa catgaggaaa tatattccac
       61 tggtattatt tatcttttca tggccggtat tgtgtgcaga tattcatgga cgggttgttc
      121 gtgtactgga cggtgatacc atcgaggtca tggactcacg gaaggtggtg cggatcaggc
      181 tggtaaatat tgatgcgccg gaaaagaaac aggattatgg ccggtggtcg acagacatga
      241 tgaaatcact ggttgcagga aaaacggtta ccgtcacata ttttcagcga gatcgttatg
      301 gtcgtatgct cgggcaggtt tatgcaccag acggaatgaa tgttaatcag tttatggtcc
      361 gggccggggc cgcctgggta tatgagcagt acaacacaga tcccgtgctg cctgtccttc
      421 agaacgaagc cagacaacaa aaacgtgggc tctggtcaga tgctgatccg gttcccccct
      481 ggatatggag acaccgtaaa taaagcgatg gcaatcttat cattcatctg ttctcagcgg
      541 gaataaaaat ggaaaaaaca aaacaggaat ggctgtatca attacggcga tgttcatcag
      601 tcaatacgct ggaaaaaatt attcataaaa acagagacag tttatcgacc agtgaacgtg
      661 aatcatttaa ttcagcagca gaccaccggt tagcagaatt aatcaccgga aagttatatg
      721 acaggatacc aaaagaaata tggaaatatg taaggtaata gttcagcata attaattacc
      781 aggagcagat aaacaatgaa gctgattatt tttattctca ttgttctgat tattgcagcg
      841 ttacttatcc ggattatcct cagaagtgta aatcagcatt ctcctttgct gatgcaattg
      901 catgcggcag gtatacggac cggtgatgca gagcgaatac tgtccggcgg tgaatatgat
      961 gccagccatt tacgacctga acgcagacgc cgggatattc ttctggaaga ggttctgagg
     1021 caggctggta ttccgttgct cagaagccac gatgccagaa aactgctgca gatgaccgga
     1081 gaatggctga atacaacagg ggctgctcag cagtcaccgg aacatcgtag ctgacgcctt
     1141 cgcgttgctc agttgtccaa ccccggaaac gggaaaaagc aagttttccc cgctcccggc
     1201 gtttccataa ctgaaaacca tactatttca cagtttaaat cacattaaac gacagtaatc
     1261 cccgttgatt tgtgcgccaa cacagatctt cgtcacaatt ctcaagtcgc tgatttcaaa
     1321 aaactgtagt atcctctgcg aaacgatccc tgtttaagta ttgaggaggc gagatgtcgc
     1381 agacagaaaa tgcagtgact tcctcatctg gcgcaaaacg agcatacaga aaggggaatc
     1441 cgctttctga tgcagagaaa caaagattat cagtggcccg taaaagagct tcgttcaagg
     1501 aagtaaaagt atttcttgaa ccaaagtata aggccatgct catgcaaatg tgtcatgaag
     1561 atggcctgac tcaggctgaa gttctgaccg cactgataaa aagtgaagcg caaaaacgat
     1621 gtgtgtgatg atgggcttac attcttgagt gttcagaaga ttagtgctag attactgatc
     1681 gtttaaggaa ttttgtggct ggccacgccg taaggtggca gggaactggt tctgatgtgg
     1741 atttacagga gccagaaaag caaaaacccc gataatcttc agaaagtttg gcgactgaga
     1801 agattaccgg ggcccactta aaccgtatag ccaacaattc agctatgcgg ggagtatagt
     1861 tatatgctcg gaaaagttca agacttcttt ctgtgctcgc tccttctgcg cattgtaagt
     1921 gcaggatggt gtgactgatc ttcaacaaac gtattaccgc caggtaaaga acccgaatcc
     1981 ggtgttcaca ccccgtgaag gtgccggaac gctgaagttc tgcgaaaaac tgatggaaaa
     2041 ggcggtgggc ttcacctccc gttttgattt cgccattcat gtggcacatg cccgttcgaa
     2101 gggactgcgt cggcgcatgc caccggtact gcgtcgccgg gctattgatg cgctgctgca
     2161 ggggctgtgt tttcactatg acccgctggc caaccgtgtc cagtgctcca tcaccacact
     2221 ggccattgag tgcggactgg cgacggagtc tgctgccgga aaactctcca tcacccgtgc
     2281 cacccgggcc ctgacgttcc tgtcagagct gggactgatt acctaccaga cggaatatga
     2341 cccgcttatc gggtgctaca ttccgaccga catcacgttc acaccggctc tgtttgctgc
     2401 ccttgatgtg tctgaggatg cagtggcagc tgcgcgccgc agccgtgttg aatgggaaaa
     2461 caggctgcgc aaaaagcagg ggctggatac cctgggtatg gatgaactga tagcgaaagc
     2521 ctggcgtttt gtgcgtgagc gtttccgcag ttaccagaca gagcttaagt cacggggaat
     2581 aaagcgtgcc cgtgcgcgtc gtgatgcggg cagggaacgt caggatatcg tcaccctggt
     2641 gaaacggcag ctgacgcgtg aaatctcgga agggcgcttc actgctaatc gtgaggcggt
     2701 aaaacgcgaa gtggagcgtc gtgtgaagga gcgcatgatt ctgtcacgta accgcaatta
     2761 cagccggctg gccacagctt ccccctgaaa gtgacctcct cagaataatc cggcacgcgc
     2821 cggaggcatt cgcacgcctg aagcccgccg gtgcacaaaa aatcagcacc acatcccaaa
     2881 aacaacctca tcatctacct tctggtgcat ccggtccccc ctgttttgat acaaaacacg
     2941 tctcacagac gggcaatttt gtttatccac attaaactgc aagggatttc tctgtaagat
     3001 tacaaccgtt catgtcataa agcaccagcc gccaggctta cagggtgcaa tgtatctttt
     3061 aaacacccgt ttatatctcc tttaaactac ttaattacat tcatttaaaa aaaagactta
     3121 ttcactgcct gtcctgtgga cagacagata tgcacctccc accgcaagcg gcgggcccca
     3181 accggagcca ctttagttac aacacacaaa aacaacctcc agaaaaaccc cggtcccgcg
     3241 cagaactgaa accacaaagc ccctccctca taactgaaaa acggccccgc cccggcccaa
     3301 agggccggaa cagagtcgct tttaattatg aatgttgtaa ctaagcatca tcatcgctgt
     3361 cagtcttctg gctgaacgtt ctcagtacac gctcgtaagc ggccctgagg gctcgctaac
     3421 gcggagatac gccccgactt cgggtaaacc ctcgtcggga ccactccgac cgcgcacata
     3481 aactctctca tggctgaaag cgggtatggt ctggcagggc tggggggggg gtaaggtgaa
     3541 atccatcaat cagtaccggc ttacgccggg cttcggcggt tttacacctg tgctacatgt
     3601 aacctccgta taccgcctta catgcgctga cgcgcattat ttgccttgtt ccgtctgaaa
     3661 gtaatcacta tgattaaata tgattaacga ctaatcggat atgcaaatga aaaacaatac
     3721 cgcacaagca acaaaagtca ttaccgcgca tgtgccatta cctatggctg ataaagtcga
     3781 ccagatggcc gccagactgg aacgctcccg gggttgggtt atcaaacagg cgctttctgc
     3841 atggcttgcc caggaggagg agcgtaatcg cctgacgctg gaagccctgg acgatgtgac
     3901 atccggacag gttattgacc atcaggctgt acaggcctgg gcggacagcc tcagtactga
     3961 caatccgtta ccggtgccac gctgatggaa ctgaagtgga gcagtaaagc gctttctgat
     4021 ttggcgcggt tatatgattt tctggtgcta gccagtaaac ctgctgccgc cagaacggta
     4081 cagtccctga cacaggcacc ggtcattctg ttaactcatc cacgtatggg ggaacagtta
     4141 tttcagtttg aacccaggga ggtcagacgg atttttgctg gcgagtacga aatccgttac
     4201 gaacttaatg gccagactat ttatgtattg cgcctgtggc acacacgaga aaacaggtag
     4261 attgtgtgtg gggacaactt aacatagcc
//
LOCUS       DABSYT020000199         4280 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.200, whole genome shotgun sequence.
ACCESSION   DABSYT020000199 DABSYT020000000
VERSION     DABSYT020000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.200"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(102..314)
                     /locus_tag="ID725_RS24200"
                     /old_locus_tag="pgaptmp_004840"
     CDS             complement(102..314)
                     /locus_tag="ID725_RS24200"
                     /old_locus_tag="pgaptmp_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGV7853265.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            complement(447..1007)
                     /locus_tag="ID725_RS24205"
                     /old_locus_tag="pgaptmp_004841"
     CDS             complement(447..1007)
                     /locus_tag="ID725_RS24205"
                     /old_locus_tag="pgaptmp_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGV7853266.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            complement(1110..1970)
                     /locus_tag="ID725_RS24210"
                     /old_locus_tag="pgaptmp_004842"
     CDS             complement(1110..1970)
                     /locus_tag="ID725_RS24210"
                     /old_locus_tag="pgaptmp_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGV7853267.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            complement(2029..2775)
                     /gene="traX"
                     /locus_tag="ID725_RS24215"
                     /old_locus_tag="pgaptmp_004843"
     CDS             complement(2029..2775)
                     /gene="traX"
                     /locus_tag="ID725_RS24215"
                     /old_locus_tag="pgaptmp_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGV7853268.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            complement(2795..4183)
                     /locus_tag="ID725_RS24220"
                     /old_locus_tag="pgaptmp_004844"
                     /pseudo
     CDS             complement(2795..4183)
                     /locus_tag="ID725_RS24220"
                     /old_locus_tag="pgaptmp_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
BASE COUNT          909 a         1349 c          991 g         1031 t
ORIGIN      
        1 gaactggccg gtcatactgc ccgaccggat attaaaaaaa gcaaatctca tgcattattc
       61 agccttctga atccctggag taatgaaggc tgcttttttc gttaccccct tcctcggcag
      121 cgccggtggc agtgttcagc cctttttcgg gcaaactgct gccagtcagt ccggggtgca
      181 acaccggcag cccgtcgcca tgcttctgcc gcttccatcc agcgtccact gacttccaga
      241 ataacggccc ggcttccggc acgctggtaa cgccagacct gaccgttccg tgaactgttt
      301 ctgaatccat tcatctttat cactcctctt taacccgtca gtattatggt gttaaataat
      361 ctactgaatt ggcttccttt cgggtacgga agttatttcc ttttaagcct tatttccctg
      421 cctcctctct ctgagggagg ctttttttat ttctcatcaa gcacggcctg aagttctgcc
      481 tttatccggt tctggcggcg gattttatcc agacgcgctg ccgcatacgc ttcctcctcc
      541 tgagaaatat gctccgtcac atacccttcc gtgtcatacc ggcaggcacc ggctttcatg
      601 gcacacagat agctttctga acgggtgatg gccttcagcg ccctgcgcag ctttttatgc
      661 gagagcggga tattccgctg cgccacgtct tccagtaaca cgtcccggat accgcaggcc
      721 agaagccggg gcgtgtcacc gtcaaataat cccggccacc agggtttcag ggtgttcacg
      781 gcctcatcct gcgtgggcag gttcagataa atggacagcg cctgtctggc ctgcgctttt
      841 tttgccgcca gctccgcttc ccgggctgct ttctcggcca gcttctgctt tttcaccttc
      901 cattttggtg gcgtggtgac attgatgatg gttttccggc tgcggacagg cgtctctcct
      961 tccgttttcc gcttcagtgt cagtaccggt cgtttttgct ctgccatgct tactctgacc
     1021 ttcgtgaaaa tatgccctgc aggccgttga aacggctcat cgtaacagga agttaatttc
     1081 aacgaccttt aacgtataaa tactgacaat taaagatatt gtttaaacca ttgtgtctgc
     1141 acagctacta cacgttcaaa aaacgcgccc tcataaatat cataatggca tgcatcagct
     1201 tcctcgtata attccttagt accagaagca acagcatcat ataatgctct tccctgttcc
     1261 ggtggattaa cgctatcctg gccggcaata acaaccaaaa ctgggcactg gacgctggct
     1321 gcactctctg ccggtttata ttgcaacgtt tccatgaccg taaggaaagg tatttttata
     1381 tccatttcgg gatgccgggc tttgattttc tcgaagaata ccttcgattc atcatcactt
     1441 agtacccgtg taacccccac aaacatctct ttcccggtgt ttttcttctt ctctgccatt
     1501 ttattcagcg tggatagaaa agacgctctc tccgattcat tcatctctcc ggtaaccaga
     1561 acatcaccgt cagcaaaagc cagctggctg acaatacatt ttacgcgttg gtcctgcgca
     1621 gctgcactga agacatgccc ccctcccagc gatgttcccc ataacccaat acgctgatta
     1681 tcaatacatt cctgcttttc tgcccaattg ataactgaaa taatatcttc ggtctgcatt
     1741 gcaggaacca gacgccctcg ttctccatca ctctcaccga atcctcgata atcaaaagta
     1801 atcgttgcga atcctgcttc tgtgaaggca ttcgcaaagc aagggagcag aacattcctg
     1861 ataccgcaaa acccatggca taagataatc aaaggatgtt ttatatttcc ttcaggaaca
     1921 cgaaatgtca gggcaatacc ttcagagagt ttgtgatccg taattttcat aaatatcctg
     1981 tgttatgttg tttattaata agcagaatca gcgtcagtat cataccagtc acaacgccag
     2041 aatgcccagc acagccagat gacagccata gaacgtcggg aaaaaatctc cgggccagaa
     2101 acggggtaat gatttccctg cacaggacac cagaccaacg gtcagcaccg tcatcacaag
     2161 accagctaca gccgccgcat cactggaggc aaggttaagt gccggaatca cggccagcag
     2221 gcatgcaacc agcaccagac gttccgttct gtcttccgcc cggtacagcc ggtggctgac
     2281 tgccagcatc agcagcccgg caatgccgta actggtgccg gacaaaggcc cccacagtgc
     2341 catcaggaga atggccgctg ctgtacgcca cccactgcgc gtttcacacc acgtcagcac
     2401 ctgtgccgcc actgcaaagg caaacaggat attcccctca taccacggaa agcctgccag
     2461 ataatacgcg aactgggcaa taattcccca tccccacagc cggttaatgg ccgactgcct
     2521 gatatgcgca tggcgggaca gattcagccc ccacaccaga gcaaacaacg gaaaggctcc
     2581 acgcccggcc agaaacatcc acgtctggtc cagatgcaga atgcggtttg cgtggtccag
     2641 taccatcagc accagtgcca ccgttttgat gatatcccgc tgtccgggtg accagaccag
     2701 caacgactgc aaccaggtat cggtccgggc agccagtgaa tcgttacgtg tcgtgttcgt
     2761 gttatccgtt gtcatcagtg tttgtcttcc tgtatcagtc tccgcccagg gttttctctt
     2821 tctgcaggtc acgaaccata tcccgctcca tctgctgcag tcgctcctgc agcaggtttt
     2881 cccggacaac ctcacggacc gcctcccgct cccgttgtgg ttcacgcagt acactctccg
     2941 gcagcgcggc ttcccgttca gaagtgacag cccgttcacg ctcctgcccg gcaatctccc
     3001 tgacagcctg ctctgttctg ccgtccggca ggtcaggttt gttctctgcc attttacgga
     3061 caatttcatc tgcgcggcgt tccgtttcac ggcggatggc ctcttcagcc tgccgctgtg
     3121 ccagtacctc tgccgtgacc ggctcgccat ttccggctcc cggctggacg ctgttgtccg
     3181 ggatatcccc ccacacgcga ccaccggtga tggcaccggg attccacgga cgaccttcac
     3241 cggcgattct caccaccaca ccgctgtcag gattatcacg ggcaatccgg acaccatcct
     3301 gcatattatc agccagcagg ctctctccgt tacggctgcc ctgcagggcc acgaactgcg
     3361 catccccgct gcctttcacc cgtccttcac cactgaatcc ccgcagcccg tttccgtcat
     3421 ccgtggtcag cgggttcagc cagataccgg cagacttgcc gttacggtca aacgccggca
     3481 gtgccacata cggctgtgga tatttacgtc ccggagcaat aaaccgtgca ggactgtctc
     3541 ccccggcaag ccccgcctga cgaagaacgg cacgccctgc cgccacgtcc cgcagctccc
     3601 gcgccgtact gaacagccgc tctgcattca tgacctcccg gtccggtttc ggttcaaaca
     3661 catcgtgggc tgttcctttc tgtacggcat tgttaatggc atccgtccag ccctgacggt
     3721 tatcggtgta cacctgcaca tgctgcttca tacgcgacag ggccacgtag gctgactcaa
     3781 agccggccat cagtttccgg ctgccttccg taccttcaag cgcgatggca aaggtttcac
     3841 ttgccccctg cgcaccgtga gcggtgatgg cataggccag gtcaatatgt tgctctgccc
     3901 gctcctgggc ggggcgaatc acccgggtct gctgtccgcc cgacagcgtg acactgtcac
     3961 cggaaaccgc tgtcaccgtc cagacgctgt tggccacata accacgcccc cggtcactct
     4021 tcgtgaagcg catccggtca ccggtcccca ccctgatggt gtccgggggg tacagtgtga
     4081 cgccttcagc caccgcctcc cggggcgaaa tcagccgcgt gttaccttcc gcatcctgca
     4141 gagttatcag cccgtcatcc ctgctgatac cggcaatccg gtgattgaac cgccccggaa
     4201 atcctggaga ctaaactccc tgagaaagag gtaaacagga tgactaaaaa tactcgtttt
     4261 tcccccgagg tccgtcaacg
//
LOCUS       DABSYT020000200         4264 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.201, whole genome shotgun sequence.
ACCESSION   DABSYT020000200 DABSYT020000000
VERSION     DABSYT020000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.201"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            175..911
                     /locus_tag="ID725_RS24225"
                     /old_locus_tag="pgaptmp_004845"
                     /pseudo
     CDS             175..911
                     /locus_tag="ID725_RS24225"
                     /old_locus_tag="pgaptmp_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1090..1386
                     /locus_tag="ID725_RS24230"
                     /old_locus_tag="pgaptmp_004846"
     CDS             1090..1386
                     /locus_tag="ID725_RS24230"
                     /old_locus_tag="pgaptmp_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7853269.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1694..1810
                     /locus_tag="ID725_RS24235"
                     /old_locus_tag="pgaptmp_004847"
     CDS             1694..1810
                     /locus_tag="ID725_RS24235"
                     /old_locus_tag="pgaptmp_004847"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853270.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            2029..3414
                     /locus_tag="ID725_RS24240"
                     /old_locus_tag="pgaptmp_004848"
     CDS             2029..3414
                     /locus_tag="ID725_RS24240"
                     /old_locus_tag="pgaptmp_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGV7853271.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIMIN
                     ELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHCREYAAKNAEYMA
                     RLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPE
                     ELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEG
                     KTLCVAPNFDL"
BASE COUNT         1271 a          797 c          935 g         1261 t
ORIGIN      
        1 atataaaatt ttaacattat atcattttgc ttttctgtta actggttttc atttttgaat
       61 caatattttc tgaacagggt aatagtacac gtattaacag attatgtcag aaggccccgg
      121 tgatgccttc tgacgataga ttaatgttgt attctattgg tatctggaga ccatatggca
      181 acagttacag tatattctcc ccgctgtaat tcctataaag tgtaccgtta tgatcacagc
      241 acctcagatc atgagcgttt ccagtgctaa tcctgcaaac gtgtatttca actgaattac
      301 atctacgaaa cccgaaaacc aggcattaaa gagcaaattg ttgaaatggt tcataacgga
      361 gccggggttc gcgatagctc cagaacgctg aaggtggata tcaacaccgt cattctcact
      421 ttaaaaaacg ctcaccacgt aaagtaacca tatctccagc cgccagtgtc gatgtggcac
      481 ttatctgtga acttgatgaa caatggagtt ttgtcgaaaa caaagctcgt cagcagtggc
      541 actggtacgc gtataagacc aaagctgacg gtgtgctggc ttacactttt ggtcctcgca
      601 ctgatgaaac atgccgtgag ttgccggaat tcctgaagcc attctccgca ggcatgataa
      661 ccagagacaa ccggagcagt tataccagag aaatgccaca ggataagcat cttgtcggca
      721 agatatttac ccggcgcatt gagcgcaaca atctgacact tcgtactcac atcaaacgac
      781 ctgcccggaa aacgatttgc tttttacgct ccctggaaat tcacgaaaag ccattggttc
      841 atttatcgaa aacacatgta ctactgacag gagtcattac cagggcctct tttgctgttt
      901 ttttgccatg aatgataatg agaatcgaca gcgataaggg aaaagtaaaa taacactaat
      961 gctgtcgttt tctatcatat tgatttttaa ttatttattg tcttttttct caacccattt
     1021 ttttacgcag acaaatttac ccggggaata caaactgtaa cgacagttca ccacactatg
     1081 ggggataaaa tgccagatca atccggacac tgcaggactc cttcacgcat cttacggatg
     1141 cctgaactga gccaattgtt aggtatatct cgttcgacaa tttacgaaaa aatgaatccg
     1201 ttatcgaaat actacgatgc aaccttcccc aggccggtac gtcttggtag tggttctgtg
     1261 ggctggcgtt catcagctat tgacgaatgg cttactttac atactgtacc tgccagatca
     1321 gcagtcaaag gtgttaatga cgaagtgaga catacgcttt cttcagatat gaccatgagc
     1381 agataagttt ttgtcatcac gtgatcacct caggaggaaa aagaagcata agaacaattt
     1441 agccagaagt ttaattgata aacagaattt ccagcattca ttgttgaaag agcagctatt
     1501 gtcgctattg tagcggtaat tgctgctata accgaaacgt attacgaaaa aggaagcatt
     1561 tatgccttac atcaaaccgg atatgtcgta ttcagtatat gcatgaccat caatgatagg
     1621 gaaggcaata attaaagctc aatacaataa acaagtacct ctgactctgg ttgcaatgtc
     1681 agtattaaca gcgatgtcaa ttgcctgtca gaatcaggtt gacgtatgca gtcccggaaa
     1741 tttacgtggg gccgttaata tttacactat ggttctggag aaagggaaaa gaccggggga
     1801 aaggtattag tgaaataatt tccggaggta ttaagaatga gaaatatgaa tgatatttcg
     1861 tggtgaataa tacagtcttc actgaattaa ccttatcggt ttattaaaca tcagatctac
     1921 aaatgctctg tatttctcag agcatcaggt ctgaatagca gctattttcg ctattgtagc
     1981 gggatttgct gctattttac taacccatta atgaaaagag aggcttttat gtgtttactt
     2041 gcaccagaaa atccttatcc gatatatgca ctaccaccgc tggtgagaaa tgcaataatt
     2101 gaaactcaaa aaaatacaca ggcacctttg gctatggtgg caacatccgc attaacagcg
     2161 atctcaattg cctgtcagaa tcagattgac gtgtgcagac ctggaaattt acatgggcct
     2221 gttaatcttt actctctgat tctggctgat tccggtgaaa ggaaaacgac cgtggataaa
     2281 gtgtttatga aagcatttta tctcagggat gaagccctgg cggatgaata cgcgaaactg
     2341 gttgagaatt acagtacaga aaaggaaata tgggagcaaa aacaaaaagc gctggaatca
     2401 aaatttcata aagagattcg tgccggtaaa gattacaagg caacagaatc agagcttgaa
     2461 acgcatctga ataaacctcc tgttccgccg cagatacgtc gaacaatttt taatgagaca
     2521 acgatagagg gaatgttaaa atattactcc gatagcaatc gctcttttgc tcttgtatcc
     2581 agtgaagggg gggtaatttt tgacagccgg gccatgagta aactgggaat tattaacact
     2641 ttgtgggatg gaggttctct tttcatcgac aggaaatcat ctcccggaat taatttgaag
     2701 gagccaagac tgacgatatc ggtgatgatt cagcctgatg tttaccacaa aggtttttgt
     2761 acgcgaaaaa aagaaattgt gaaaacgtca ggacatcatg caaggttttt gatgtgtcaa
     2821 ccaacatcaa cgcaggggac aaggataatg atcaatgagc ttattgatga aagtctggcg
     2881 atgagtggtg aacgacgttg ccttcacttt tctcctcagg ctgccagaat ctggacggat
     2941 tattacaatg atgtggaatc gaagctgggg ggattggggc ctttaagaca ttgccgggaa
     3001 tatgctgcta aaaatgcaga gtatatggca agactggcag gacttattta ccattcgagc
     3061 ggtgaagagg gggagatttc cccttacact gcagaaatgg cgagagaatt agcaatatgg
     3121 tacggtaatg agtatgtgcg gttgtctaat ccgttaactt ttgacaactc tgccctgacc
     3181 gtacctgtgc gactcattcc agaggagctt gaacttttca actggataaa aagctattgt
     3241 attgagaagg ggatcctctg tatgaaaaaa aatgacattt tacagcgtgg tccgaaccgt
     3301 ttccggaaaa aggataaaat caactggtta ctggatttac tgtatgaaca aaacagagtt
     3361 gtaccggtta ttgagggaaa aacgttgtgt gtcgcaccta actttgacct ctgaacgact
     3421 taatcgttga gtacatatag tctcctctgc tacttgcgga ggagactaat gtctttacca
     3481 tgtcagcgtt gtattagaaa actctggtac aggtcagtca gtaaatataa ctggtgtaac
     3541 cactatttac tattggatag agtgttgctc aggatattgt cggctttagt ctgaggtgag
     3601 gtggggcctt cattgttttc gtgttcaata aataatttag gttaattagt attattagtg
     3661 gaagttatgt gatctgttat gttgtcttgt acgcacttaa cactggttac gtaccggaac
     3721 actattagag tatgttatgg ctctgttaca gtttataggt atgaaaaaat ataatctttc
     3781 ctgtatgaaa taatattctt atggtgtgtg gggtatggtc tgtataccat catttttatg
     3841 gtgaagttaa atatctgtag aggccttaag ctgggtcgct gtttttactt aatggtacag
     3901 aagtattaat agcggtgttg cctgtggcta cccgatactg gcttttgtga atgtcagatt
     3961 acttcctgta agtgattgaa tggtacttcc ttaaactcaa ttctatgagg tgtgttttta
     4021 ccattgtaat tcgtactggt cctcagttta agtgagtctt actgtactct gaaatattac
     4081 ctctttggag taatgaggta atacgaaaca gttttatcag gttgtctgtg ctgctggtta
     4141 cctcgtttat taatgccgcg gtacggcgtt tctgtactct cagtatttat gtaattccac
     4201 cttcctgtaa cttttataaa gtaaggacta taaaatgtat aatgctaatc ccaattacga
     4261 aatg
//
LOCUS       DABSYT020000201         4233 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.203, whole genome shotgun sequence.
ACCESSION   DABSYT020000201 DABSYT020000000
VERSION     DABSYT020000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.203"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..109
                     /locus_tag="ID725_RS24245"
                     /old_locus_tag="pgaptmp_004849"
     CDS             <1..109
                     /locus_tag="ID725_RS24245"
                     /old_locus_tag="pgaptmp_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853272.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGAWA"
     gene            100..588
                     /locus_tag="ID725_RS24250"
                     /old_locus_tag="pgaptmp_004850"
     CDS             100..588
                     /locus_tag="ID725_RS24250"
                     /old_locus_tag="pgaptmp_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGV7853273.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSAFKNRKRVEKIKH
                     VA"
     gene            902..977
                     /locus_tag="ID725_RS24255"
                     /old_locus_tag="pgaptmp_004851"
     tRNA            902..977
                     /locus_tag="ID725_RS24255"
                     /old_locus_tag="pgaptmp_004851"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:935..937,aa:Ile,seq:cat)
     gene            979..1055
                     /locus_tag="ID725_RS24260"
                     /old_locus_tag="pgaptmp_004852"
     tRNA            979..1055
                     /locus_tag="ID725_RS24260"
                     /old_locus_tag="pgaptmp_004852"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1013..1015,aa:Arg,seq:tcg)
     gene            1069..1145
                     /locus_tag="ID725_RS24265"
                     /old_locus_tag="pgaptmp_004853"
     tRNA            1069..1145
                     /locus_tag="ID725_RS24265"
                     /old_locus_tag="pgaptmp_004853"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1103..1105,aa:Arg,seq:tct)
     gene            1238..2197
                     /locus_tag="ID725_RS24270"
                     /old_locus_tag="pgaptmp_004854"
     CDS             1238..2197
                     /locus_tag="ID725_RS24270"
                     /old_locus_tag="pgaptmp_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGV7853274.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            2209..2478
                     /locus_tag="ID725_RS24275"
                     /old_locus_tag="pgaptmp_004855"
     CDS             2209..2478
                     /locus_tag="ID725_RS24275"
                     /old_locus_tag="pgaptmp_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGV7853275.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            2965..>4233
                     /locus_tag="ID725_RS24280"
                     /old_locus_tag="pgaptmp_004856"
     CDS             2965..>4233
                     /locus_tag="ID725_RS24280"
                     /old_locus_tag="pgaptmp_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGV7853276.1"
                     /translation="MAFKHYDVVRAASPSDLAKRITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAVCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSPSGAEATG"
BASE COUNT         1094 a          941 c         1113 g         1085 t
ORIGIN      
        1 agcagtgaaa ccactcaaat ttgccaacca gatgtatatc gataccggcg cagtgttctg
       61 cggaaatctc acattgattc aggtacaggg agaaggcgcg tgggcataag agaactaaac
      121 ctcaccaaag aacagcatga gtggctgaat ggctggcttg aactgtgggg cgcatgggtt
      181 tattcaggtc gtctggaaaa gcgcatgagc agcgtaatag ctaaattcat ggagagcgtg
      241 gagccgggaa gagttatgac aaggccaatg tgtaatgatg atgatggaat gttgatttct
      301 caggtcgtcg attccgtcat gtacattgac aagaaagcct ttggcatcct cctcagctac
      361 tacgcccacg gctcttccaa gcacgccatt gcatcttact atcatagcgt cgcaagacct
      421 cgcaagatgt tatgccgggg cggcgggcgc attcaaaaac catcgctagc aacctgccga
      481 cgggaagttg acgaaatcct caatgcctcg ttgtttatga tttacccgat tctggatagt
      541 gcgtttaaaa accggaaacg tgtagagaaa attaagcatg tagcatagaa cgtgttgaca
      601 tcgttgagca aatgagcaac actattcgca taagctgccg ttagtgactc ttaagttgca
      661 acggtggctt tttttatttg ggtcaatcgt ataaaggtca ttagagcctg taactgtttt
      721 tcgtccataa attaaccttc atttgatgct ggattgaaca tatcaaaatc aggcaattac
      781 acaaatctat gtacaggctc tttgtgcctg acgttaaaag attttttaca agaataattt
      841 tgaatcagtg aatttgtgaa ctcttgcaac attgatttcg taacgttatt atcctacgct
      901 cggcccttta gctcagtggt gagagcgagc gactcataat cgccaggtcg ctggttcaaa
      961 tccagcaagg gccaccaacc gccattagct catcgggata gagcgtcagc cttcgaagct
     1021 ggttgcgcga ggttcgagtc cccggtggtg gtccattatc ggtattctgc gttgttagct
     1081 cagcaggaca gagcaattgc cttctaagca atcggtcact ggttcgaatc cagtacaacg
     1141 cgccacactt attttccctg gctcgctttt gcgggccttt tttatatctg cgccgggtct
     1201 ggtgctgatt acttcagcca aaaggaacac cttgtatatg aagtgtatat tatttaaatg
     1261 ggtactgtgc ctgttactgg gcttttcttc ggtatcctat tcccgggaat ttacgataga
     1321 cttttcgact caacaaagtt atgtatcttc gttaaatagt atacggacag agatatcgac
     1381 ccctcttgaa catatatctc aggggaccac atcggtgtct gttattaacc acaccccacc
     1441 gggcagttat tttgctgtgg atatacgagg gcttgatgtc tatcaggcgc gttttgacca
     1501 tcttcgtctg attattgagc aaaataattt atatgtggcc gggttcgtta atacggcaac
     1561 aaatactttc taccgttttt cagattttac acatatatca gtgcccggtg tgacaacggt
     1621 ttccatgaca acggacagca gttataccac tctgcaacgt gtcgcagcgc tggaacgttc
     1681 cggaatgcaa atcagtcgtc actcactggt ttcatcatat ctggcgttaa tggagttcag
     1741 tggtaataca atgaccagag atgcatccag agcagttctg cgttttgtca ctgtcacagc
     1801 agaagcctta cgcttcaggc agatacagag agaatttcgt caggcactgt ctgaaactgc
     1861 tcctgtgtat acgatgacgc cgggagacgt ggacctcact ctgaactggg ggcgaatcag
     1921 caatgtgctt ccggagtatc ggggagagga tggtgtcaga gtggggagaa tatcctttaa
     1981 taatatatca gcgatactgg ggactgtggc cgttatactg aattgccatc atcagggggc
     2041 gcgttctgtt cgcgccgtga atgaagagag tcaaccagaa tgtcagataa ctggcgacag
     2101 gcctgttata aaaataaaca atacattatg ggaaagtaat acagctgcag cgtttctgaa
     2161 cagaaagtca cagtttttat atacaacggg taaataaagg agttaagcat gaagaagatg
     2221 tttatggcgg ttttatttgc attagcttct gttaatgcaa tggcggcgga ttgtgctaaa
     2281 ggtaaaattg agttttccaa gtataatgag gatgacacat ttacagtgaa ggttgacggg
     2341 aaagaatact ggaccagtcg ctggaatctg caaccgttac tgcaaagtgc tcagttgaca
     2401 ggaatgactg tcacaatcaa atccagtacc tgtgaatcag gctccggatt tgctgaagtg
     2461 cagtttaata atgactgagg cataacctga ttcgtggtat gtgggtaaca agtgtaatct
     2521 gtgtcacaat tcagtcagtt gacagttgcc tgtcagactg agcatttgtt aaaaaaattt
     2581 cgcatggtga atccccctgt gtggaggggc gactggtgaa aaatccttgc ttgtgattca
     2641 ttatcgacac gggttcggtg gtaccaggcc gaactcaccg ggaggcaccc ggcaccatgc
     2701 agtatacaga gattaggcat ataccaaggc ctctcatagc aggggccttt ttacatgtaa
     2761 aaaagcccga gtgggttcgg gcaattgcat gagatactcg ttttaataat cgaaatcatt
     2821 ttaaccagga ttcataaggc tgcgcaactg cgcgaccttt ttcgaattgc gggctgtagt
     2881 ctcccttctg ccattgtcct gtaacttccg gacttcagcc cgctccttat tttactcaca
     2941 atattatccc ggccgggagg attcatggca tttaaacact atgatgttgt cagggcggcg
     3001 tcgccgtcag accttgcgaa acgaataact caaaaactga aggaagggtg gcagccttat
     3061 ggtagtgcgc tgatttcgac agctggttat ggtgcggagt tcatccagcc agttgtgagt
     3121 gaggggagca tctcatcacc agaggagcca ggcaaccgtc cgacgacctc agcgccttct
     3181 gttgcgccag aatattacta tgtgatcgcg cttgctggtc agtccaatgg tatgtcatac
     3241 ggtgagggac tgccattgcc ggatacattc gacagccctg atccacgtat taaacagtta
     3301 gcgcgtcgca gtacggtgac accgggcggt gcagtatgca aatataacga catcattccg
     3361 gcggaccatt gtctgcatga tgtgcaggac atgagccgtc ttaaccatcc gaaagcggac
     3421 ctgtcaaagg ggcagtacgg aaccgtgggg caggggctgc atatcgccaa aaaactgctg
     3481 ccgtttatac cggcgaatgc gggcattctg ctggttccgt gctgtcgtgg tggttcagcg
     3541 ttcaccaccg gagccgatgg cacatacagt gacgcgagtg gtgcctcgga gaattcaacc
     3601 cgctggggtg tggacaagcc gctgtataag gaccttatcg gtcgaacaaa agcagcactg
     3661 aagaagaatc cgaaaaatgt gctgtttgcc gtggtgtgga tgcaggggga atttgatttt
     3721 ggcggtacgc cggtaaatca cgccgcacag tttggtgcgc tggttgataa attccgtgca
     3781 gacctggcgg atatggcagg ccagtgcgtc ggtggctctg ctggcggtgt tccctggata
     3841 tgcggggaca cgacgtattt ctggaagcag aagaacgaat ccacgtacca gacggtgtat
     3901 ggcagctata aaaacaaaac ggaaaagaat atccatttcg taccgttcat gacggatgag
     3961 aacggggtga atgtgccgac gaacaaaccg gaagaagacc cggacattcc gggtatcgga
     4021 tattacggtt cgaaatggcg tgacagctca gccacctgga cgtcacagga cagggcgagc
     4081 catttcagtt catgggctcg ccgcgggatt atttccgacc gtctggcaac ggcgattttg
     4141 cgccatgcgg gaagagtggc gctaaacgcg ggggcatcat cgacagtatc agaggtgcgc
     4201 ccgtcatcgc cttccggtgc agaagccaca ggc
//
LOCUS       DABSYT020000202         4217 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.204, whole genome shotgun sequence.
ACCESSION   DABSYT020000202 DABSYT020000000
VERSION     DABSYT020000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.204"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..209)
                     /locus_tag="ID725_RS24285"
                     /old_locus_tag="pgaptmp_004857"
     CDS             complement(<1..209)
                     /locus_tag="ID725_RS24285"
                     /old_locus_tag="pgaptmp_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGV7853277.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ID725_RS24290"
                     /old_locus_tag="pgaptmp_004858"
     tRNA            complement(324..399)
                     /locus_tag="ID725_RS24290"
                     /old_locus_tag="pgaptmp_004858"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ID725_RS24295"
                     /old_locus_tag="pgaptmp_004859"
     tRNA            complement(406..480)
                     /locus_tag="ID725_RS24295"
                     /old_locus_tag="pgaptmp_004859"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ID725_RS24300"
                     /old_locus_tag="pgaptmp_004860"
     tRNA            complement(597..681)
                     /locus_tag="ID725_RS24300"
                     /old_locus_tag="pgaptmp_004860"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ID725_RS24305"
                     /old_locus_tag="pgaptmp_004861"
     tRNA            complement(690..765)
                     /locus_tag="ID725_RS24305"
                     /old_locus_tag="pgaptmp_004861"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ID725_RS24310"
                     /old_locus_tag="pgaptmp_004862"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ID725_RS24310"
                     /old_locus_tag="pgaptmp_004862"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGV7853278.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ID725_RS24315"
                     /old_locus_tag="pgaptmp_004863"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ID725_RS24315"
                     /old_locus_tag="pgaptmp_004863"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGV7853279.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ID725_RS24320"
                     /old_locus_tag="pgaptmp_004864"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ID725_RS24320"
                     /old_locus_tag="pgaptmp_004864"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGV7853280.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1082 a         1047 c          945 g         1143 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc aaccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggcct
//
LOCUS       DABSYT020000203         4169 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.205, whole genome shotgun sequence.
ACCESSION   DABSYT020000203 DABSYT020000000
VERSION     DABSYT020000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.205"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(663..863)
                     /locus_tag="ID725_RS24325"
                     /old_locus_tag="pgaptmp_004865"
     CDS             complement(663..863)
                     /locus_tag="ID725_RS24325"
                     /old_locus_tag="pgaptmp_004865"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HGV7853281.1"
                     /translation="MALELSYVYIKYVYGKEKAEFQKPYSITDDNNCWKIEGKQPKNS
                     GGNFTMLIAKKDGQVLNVIHTK"
     gene            complement(1134..1703)
                     /gene="ybfC"
                     /locus_tag="ID725_RS24330"
                     /old_locus_tag="pgaptmp_004866"
     CDS             complement(1134..1703)
                     /gene="ybfC"
                     /locus_tag="ID725_RS24330"
                     /old_locus_tag="pgaptmp_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HGV7853282.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLTKIQHELLRSEEK"
     gene            complement(1700..>4169)
                     /locus_tag="ID725_RS24335"
                     /old_locus_tag="pgaptmp_004867"
     CDS             complement(1700..>4169)
                     /locus_tag="ID725_RS24335"
                     /old_locus_tag="pgaptmp_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGV7853283.1"
                     /translation="RGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWG
                     KAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHH
                     DLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHR
                     VTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWL
                     TYGSGWLAGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSLQY
                     DRDYTWNDNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNRLP
                     DPELHPDSTLSMWPDNRIARDAHYLYWYDRHGRLTEKTDLIPEGVIRTDDERTHRYHY
                     DSQHRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWY
                     GWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGED
                     GGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKI
                     HLYHCDHRGLPLALISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESG
                     LYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGY
                     GARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWV
                     KPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPA
                     TAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          908 a         1056 c          978 g         1227 t
ORIGIN      
        1 ctgttgtacc ataggctatc cttgagatta aattaacctg aattctaatt tgtttactcc
       61 ataaatcaaa ttcaattttt ttattgctcg atcaccaaga tatattctat caaacatatt
      121 acccccctat tctaaaaata ctcaggcatt ccgcacatta tatcagcctc atcttgattg
      181 cttcccgttt cccgatacgg cggcgcgggg agttataaag gtaagaaatt tccagcagta
      241 cggcgtccta ttgttcacgg cggtaatgca ccagccagta ttggctatcg tagtgataac
      301 cgtgtaacct gccgccgcat cgcgtatcta atgttcattc ttttccataa accgtccgta
      361 cacattgtga tagtaaaaat acatcttcgc tgatgcggtt attctgctgc atgtggggag
      421 cgccgaatac tgctcccggt atggccagta attactattt agtacgacgt tagggggagt
      481 cacttaatct cacagatact tcaagtaaca gatgaaaatc gctcctgaaa tgaaaaaaga
      541 aattttttga atgtattcct gtcaaatcaa gtggtttttt cgtcgatatg tagagtcata
      601 ttgtacacaa aattgttacg aaacacacct tggtaaaata tttgaatcga ctgtacagtg
      661 gattattttg tgtgtattac attcaacact tgcccatctt ttttcgctat tagcattgta
      721 aaatttcctc ctgaattctt cggttgtttt ccctcgattt tccaacaatt attgtcatct
      781 gtaatactat atggtttctg aaattctgct ttttctttcc cgtatacata tttaatgtaa
      841 acatatgata attccaaagc catttcacga ctatttactt aaatttccac tggattttct
      901 tttccaaata ctgagtgtga tattgtaaac aatatcataa taagtaatat tagtgagttt
      961 ctcatgttct acacctatcg aatctcaaaa gttgtgggta aaacattaca ttcagaatat
     1021 atgatattct taccatcgga atccattttt ccaaatcgac cttctggtgt acctggatat
     1081 ccattaagaa catgtttttc cgaatggtta atttattttc tcaggaagat gttttatttt
     1141 tcttcactac gaagcagttc atgctgtatt tttgttaaac attcgtaaga ctcatctttg
     1201 ttcgggagat gtgctgtcat tcgagcataa gttggcttct catctttaaa atatttaacc
     1261 atgattgaat tatcaaggac tgccaacaac tgagctggat cactgctctt tttttgatag
     1321 atacccgctc ttatataact tctaaaccga tcgccgagaa acgccgtttt ttctgctgta
     1381 tttatcaact cctgcaacgt tcctttgatg gcatgatttt cattaaggcg tagtgacccg
     1441 acacgaatta agagtctcgc cccgcttggt gtgaatttat tatttttaca tgtacccagt
     1501 tctgtttgcc aataatactc accgatgtcc tcattctgat accatctcca catggcttta
     1561 ttatttaaga caggaaacgc ggaaaaaata gtacccttag cgaaaatagt aaaacaatca
     1621 tgatctttag cttgtatttc gcaatcagct ataacaccaa aactaacaaa tatcatcagc
     1681 aaaaaaaaca aaactctttt cattttctta ttcctctcga tgttctctgt ttcattttag
     1741 ctaaaatttc acccaaatca tctatccctg ctgttgctgg cccatcgggt ttattaatgt
     1801 cagggaataa atagggttct gcacaagtat atcctggtgg taattgagtt gttggccaat
     1861 ttgtctgtgg caggtaatca cttgctgtct ttgtatccat ccctggatac tggggatcat
     1921 cctgtggcgt acaagtcaga tttggtttta cccacttcct gcaatacagt gtgtagtcgt
     1981 aagggcttaa tgctctgaag aatgttttga aaacattagg cgcactactg gcctggtcaa
     2041 taggatatgg cagtcttaat tgtttcgcta tgtctggaaa tttacttgga tcgggcgtag
     2101 gcggtttgtt gggtctcgct ccatatccgc cattatttaa gtgtccatat tttgatgcca
     2161 gtcccattga atctatatac tgaaccggat tcaacggata ctgataaaaa ttccatcccc
     2221 ccttcagtcc aatcggatcc tgagtgatat acctcccctg cagcgggtca taatagcggt
     2281 ggcggttgta atacaggccg gactcctcat catactgctg ccccggcagg cggataagct
     2341 gctgcagctg atgcgggttc tcttcattca gcaggttgcc ccactcatcg tattctgcgc
     2401 accattctgt tgccccttcc ttactgataa gggccagcgg caggccgcga tggtcgcagt
     2461 ggtacaggtg gatttttcgc gccggcgtgt ataccgggtc catctggctt tgcatctgcg
     2521 ccacagtcag gccgcacgat gccagccagc ggcggctttc ctcactcacc cggtcagcca
     2581 ggatttcact ttccagccgg tcgagcatct gcaccagcac cggcgggaac accacactgc
     2641 caccgtcttc gccgccggac tgctgaagcg catccgccag gctgcggcgc tgcgttttcg
     2701 ccagctcacc ggtggcggtt tcaaccctga tgagtggcgt gaagctcccc ggctgataaa
     2761 tcgtctggat gcgggttctg tcgttctgta ttgtggtcag ccggtcgccg tcccagccgt
     2821 accaggtcac ttgcggtttc cgtgacagcg acatccagcc cgtcaggtcc cgttcacgtc
     2881 gccacacccg ttttgccacc ctgcggccca gcgggtcgta aagatagcgg ctttcgacca
     2941 gcggctcttc atattgtgtc cgcgtgtagt gcaccagccg gtgctgactg tcgtaatggt
     3001 accggtgagt ccgctcatca tccgtgcgga taaccccttc cgggatgagg tcggttttct
     3061 ctgtcagcct gccgtgacgg tcataccagt aaagatagtg cgcgtcacgg gcgatacggt
     3121 tatccggcca catgctgagg gtgctgtccg ggtgcagctc cgggtcgggc aggcggttac
     3181 ctgccgggtc cgtggcatac gggatgcgga tatccagatt cgctgcggtg gtgtgaacgc
     3241 cggtcagcct gccggagtcg ctgtagctgt aactccgggt ctggcgcggg ctgctgatgc
     3301 ggatgagttc gccgttgtcg ttccaggtgt aatcgcggtc atactgcagg ctgttcaggt
     3361 gctggctctg taactgcccg gcaggggtat aagcggtggt gagttcataa cggccgaatc
     3421 tgcgcagcgt tttccggtgc aggcggtcgc gggtgaaatc caccagcggt gtgtcgccga
     3481 gcttcatgcc tgccagccag ccgctgccat aggtcagcca ttccacggcg ggcaggctgt
     3541 ccggtataca gcggttcgcc agcccctgcg cgttgtaagc gtgtctggtc tcatgctgcc
     3601 agagcagtgc ttccgtctgc gggtgatgca ccgtctgacg ctcaccggtc agccggcctt
     3661 tctcatcata cccgtaatgc accgtcaccc ggtgcccttc gctgatatgg ctgatgtctg
     3721 tcagccagcc gcgttcgtca tactgccagc gctctgcggt ttcacccttc acggtgcggt
     3781 gcgtgaggcg gtctgcttcg tcatagtgcc agtgggtgac cagcccctca tcctcgctgc
     3841 ggataagttt gccggtcagg tcgtggtgat aacgctgtgt gcggccgtca aagccggttt
     3901 cctgtatcag ccggtcgagt acatcgtaac ggaaggtggt gtggctgccg ttttcactgg
     3961 tcaggcggat gacccgtccg gcagcatcgt attccatact gcgcgtcaga ccgccctgcg
     4021 tggtacagat ggcttttccc cacgcatcgt actgtgtccc gtttctgctg ccgtccgggg
     4081 caatgacggt ggtcaggtca ccggcggcgt tgtattcata ccgcgtttca tggccctgcg
     4141 tgtctttcac ggcaattaac tgtccacgg
//
LOCUS       DABSYT020000204         4160 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.206, whole genome shotgun sequence.
ACCESSION   DABSYT020000204 DABSYT020000000
VERSION     DABSYT020000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.206"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..1004
                     /locus_tag="ID725_RS24340"
                     /old_locus_tag="pgaptmp_004868"
     CDS             <1..1004
                     /locus_tag="ID725_RS24340"
                     /old_locus_tag="pgaptmp_004868"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGV7853284.1"
                     /translation="LACREKSKIAPEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEF
                     KPDLVICHSGHDSNIAGLSRLICCHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSA
                     MMAHLMAEGVRTPVTVIPPGFDWPALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLR
                     PEKGHEFMLRVLYQLKMEGKSFRWLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALF
                     PALPVYRIASVVVMPSENEAFGMVLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPV
                     GDVSAWTGALRDFLSRPERFRMMAASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            1094..2815
                     /gene="cptA"
                     /locus_tag="ID725_RS24345"
                     /old_locus_tag="pgaptmp_004869"
     CDS             1094..2815
                     /gene="cptA"
                     /locus_tag="ID725_RS24345"
                     /old_locus_tag="pgaptmp_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGV7853285.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            2889..3887
                     /gene="lpxM"
                     /locus_tag="ID725_RS24350"
                     /old_locus_tag="pgaptmp_004870"
                     /gene_synonym="msbB"
     CDS             2889..3887
                     /gene="lpxM"
                     /locus_tag="ID725_RS24350"
                     /old_locus_tag="pgaptmp_004870"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGV7853286.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            3940..4128
                     /locus_tag="ID725_RS24355"
                     /old_locus_tag="pgaptmp_004871"
                     /pseudo
     CDS             3940..4128
                     /locus_tag="ID725_RS24355"
                     /old_locus_tag="pgaptmp_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1039 a          951 c         1063 g         1107 t
ORIGIN      
        1 tacttgcctg cagagaaaaa agtaaaattg ctccggaagc cagaaaaaga gggcatgatg
       61 tcacttttat tcctttcagg aacagccttc accttccttc catcctcaga cttcgacgga
      121 ttatcgggga atttaaacct gatctggtca tctgccatag tggccatgac agtaatatag
      181 caggactgag ccgtctgata tgctgtcacc gcttcagtat tgtcagacaa aaaacctaca
      241 tcacccgtaa gaccagaacg ttttccctga attatctgtg tgatttcatt gttgtgccta
      301 gctcagccat gatggcgcat ctgatggcgg aaggtgtcag gacaccggta acggttattc
      361 caccgggttt cgactggccc gcattgcata atgaagccat gcgaccgtta cccctgcata
      421 tccacgcctg ggctgcatca gcagacaatg ttccgcttat cgttcaggtg gggatgctac
      481 gtcccgaaaa aggacatgaa tttatgctgc gtgtattgta tcagctgaag atggaaggga
      541 aatcatttcg ctggctcgtg gtgggcgccg ggcgtgaaga atacgaagca cgtctgcgtc
      601 agcagacaga acacctggga atgagcggag atgtgttaat ggcaggtgcc cttttcccag
      661 cgttaccggt ctaccggatt gccagtgtgg tggtcatgcc ttcggaaaat gaagcgtttg
      721 gtatggtact ggctgaagcg tcagtctccg gcgtgcccgt tatcgccagt gagacgggcg
      781 ggatccctga tgtcattcag aagaatgtga cagggacgct gttacccgtg ggggatgtgt
      841 ctgcatggac cggcgcactg cgcgattttt tatcccgacc ggaacgcttc cggatgatgg
      901 cagccagcgc cagagaagat attgagtacc ggtttgatat taacagaacg gcacagatca
      961 ttgtgtctct ggctagtcag gctaagggga aatgcaacag ataacggcgg atcctctttt
     1021 cagcccgaat tttgttcagc ggttttccgg taacggagta atttatcaca ttgtttaaat
     1081 acggggacgg tcaatgcatt taaataccgg acagaacagg cctactttca gctggagcgc
     1141 gctgggatgg gcaatttttt attttggctt tttttccact ctcctgcagg tcatcatctt
     1201 cagcagtggg tacagcggaa cgaatggaat acgggactca ctgttattca gttgtctgtg
     1261 gttgatcccg gtgtttctct atcctgatcg gataaaaata attgcagctg ttgtcggttt
     1321 cattctctgg ggcacgtcgc tggcagcact ttgttattat tttctctatg gtcatgaatt
     1381 ctctcaaagt gttcttttcg ttatgtttga aacgaatgcc agagaggctg gtgaatattt
     1441 cagccagtat ttcagcctta aactgttgct tatctcgctg gtatatactg cggtgtccgt
     1501 ttttctgtgg acacgtctgc gtcctgtata tattcctttg ccatggcgga gaattgtctc
     1561 tttcctgctg ctttatgctc tgcttctgca tccggttgtt ctgaaatcgt taatcagaca
     1621 ggagccgctg aatgatactc taggcaaact ggcatcccga atggagcctg ctgccccctg
     1681 gcagtttgta tccagttatt atcagtacca tcagcaactg aatgcactga caaccttcct
     1741 gaatgaaaat agcgcactgc caccactggg taatctcagg gatgaatcag gggagagacc
     1801 acgcacactg gtcctggtga ttggtgagtc gacacagcgc gaacgcatga gcttatacgg
     1861 gtatctacgt gaaacgacgc cggagctgga tgcactgcgt aaaaccgatc cgggtcttac
     1921 tgtgtttaat aatgtggtgg catcgcgtcc gtacaccatt gaagcattgc aacaggccct
     1981 tactttcgcc aacgaaaaga atcctgatct gtatctgacg cagccgtcgc tgatgaacat
     2041 gatgaagcag gcaggctata aaacattctg gattaccaac cagcagacaa tcacagcccg
     2101 taacaccatg ctcactgtat tttcgcgcca gacggacagg cagtactaca tgaatcagca
     2161 gcgaacacaa agtgcgcgtg aatatgacac taacgtgctg aagccgttcc gggaagtgct
     2221 gaatgaccct gcaccaaaga aactgatcat cgtgcatctg ctgggtacgc acattaagta
     2281 taaataccgt tatccggaag gtcaggggcg gtttgatggc attacagggc atattcccac
     2341 tggattaaat gcgaaagagc tggaagtgta taacgattac gataatgcca atctgtttaa
     2401 cgatcatgtg gtggccagtc tgataaagga cttcagagca actgcgccgg acggctttct
     2461 gctttatttt tcagaccatg gtgaagaagt atatgatact ccgccatata aaacgcaggg
     2521 acggaatgaa gataacccca cacgtcccat gtacactgtt ccgttcctgc tgtggacctc
     2581 ggaaaagtgg catgctgcac atccgcgaga tttttcgcag tatgttgacc gcaaatacag
     2641 tctggctgaa ctgatccaca cctggtcaga tttggcggga ctgacatacg atggttacga
     2701 tccgacccgt tctctggtga atccgcaatt cagggaaacc acccgctgga ttggaaatcc
     2761 gtataaaaag aatgggctca ctgatttcga cactcttccg tatggtgagc catagtattt
     2821 tcttatcttc ggttccgttc tgaaggcagg aggatgtgtt ttcctctgct gtccggtggc
     2881 ctccagggat ggtaccacct tctgctgtac tctgttatca taatgagata agcaggcaga
     2941 taccggtaaa tatgaaaaat atcagaactg agtttattcc ccgctttaac cttacattat
     3001 gcttcccccg ttactggatg acgtggacag gtatcggtat tatctgtgtg tttgcaatgg
     3061 tcccccccgc actgagagat ccattgttgg gaaaattggg catgctggtt gggcgtctgg
     3121 ggaaaagcgc acgtcaacgg gccttgatta atttatcgct ttgttttccg gagtacagtg
     3181 ataaagaaaa ggagaatatc gttgatgcca tgtttgcaac agcctccatg gccgttgtgc
     3241 ttatggctga attagcactg tccgggcctg ataaaatttc gcatcgtata cgctggaatg
     3301 gtctggagat tgtggagaag atggcacaga acaatgaaaa ggtcattttt cttgtgccac
     3361 atgcctgggg agtggatatt cccgcaatgc tgatggctgc gtcgggcaga aaaatggcag
     3421 cgatgtttca taaccagaga aatccggtgg tggattatgt ctggaacagt gttcgtcgtc
     3481 gtttcggagg aaaactgcac gcacgtaatg acggtattgc ctcctttgtc cgctcagtcc
     3541 ggcagggata ctggggatat tatcttcctg atcaggatca tggcccggaa ttcagtgagt
     3601 ttgcagattt ttttgcgacg tataaagcaa ctttaccggt tatcggacgg ctcagcagga
     3661 tcagtggtgc acggataata ccgcttttcc ctgtttatga cggaaagacc catcatctga
     3721 ctattcatgt cagcccgcca cttgctatac gacagaagag tgatgctcat attgcgcgac
     3781 agataaacga agtggttgaa aatttcgttc gtccacatcc tgagcagtat acgtggatcc
     3841 tcaagttgct gaaaacgcgt aaagaagggg aagaggatcc ttactgaggc agctttttca
     3901 ggataatgag cttacccagc aatagtggac acacgctaag tgagtaaact ctcagtcaga
     3961 ggtggctcat atgacaaaaa aacagtacca accagtaaaa aaccccgtaa atagcattcg
     4021 cctgaatttc gcagtgaagc cctgaagctt gctgaacgca tcggtgttgc tgtcgcagct
     4081 cgtgtgctca gtctgtatga atcacagctt gtaagcggct cgtcagaact gtattgatat
     4141 ttactgagcg gtcagttcaa
//
LOCUS       DABSYT020000205         4115 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.207, whole genome shotgun sequence.
ACCESSION   DABSYT020000205 DABSYT020000000
VERSION     DABSYT020000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.207"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..268)
                     /gene="escP"
                     /locus_tag="ID725_RS24360"
                     /old_locus_tag="pgaptmp_004872"
     CDS             complement(<1..268)
                     /gene="escP"
                     /locus_tag="ID725_RS24360"
                     /old_locus_tag="pgaptmp_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle length
                     regulator EscP"
                     /protein_id="HGV7853287.1"
                     /translation="MTRVSLKRNLIEPVSFRPSDDWPQGEESFNDIFYHIKKRSPDTL
                     FIRMASLWKRLFYSSGRRRRYFEEGEHSFSILCGRLRGIVLTIKC"
     gene            complement(261..581)
                     /locus_tag="ID725_RS24365"
                     /old_locus_tag="pgaptmp_004873"
     CDS             complement(261..581)
                     /locus_tag="ID725_RS24365"
                     /old_locus_tag="pgaptmp_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HGV7853288.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKENLQHVNKSVEKLSFAIKE
                     HYFD"
     gene            complement(641..1981)
                     /gene="escN"
                     /locus_tag="ID725_RS24370"
                     /old_locus_tag="pgaptmp_004874"
     CDS             complement(641..1981)
                     /gene="escN"
                     /locus_tag="ID725_RS24370"
                     /old_locus_tag="pgaptmp_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HGV7853289.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1965..3992)
                     /gene="escV"
                     /locus_tag="ID725_RS24375"
                     /old_locus_tag="pgaptmp_004875"
     CDS             complement(1965..3992)
                     /gene="escV"
                     /locus_tag="ID725_RS24375"
                     /old_locus_tag="pgaptmp_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HGV7853290.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTN
                     QQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3989..>4115)
                     /gene="cesL"
                     /locus_tag="ID725_RS24380"
                     /old_locus_tag="pgaptmp_004876"
     CDS             complement(3989..>4115)
                     /gene="cesL"
                     /locus_tag="ID725_RS24380"
                     /old_locus_tag="pgaptmp_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HGV7853291.1"
                     /translation="IFVYDNKLCLSCLLTNIDVFFLMNTFQQHVQIIERVRRMTS"
BASE COUNT         1266 a          943 c          737 g         1169 t
ORIGIN      
        1 tacatttaat tgttaaaaca attcctcgta atcttccaca aagaatacta aacgagtgct
       61 ccccttcttc aaagtagcgt cgtcttcggc ccgaggagta aaacaatctc ttccataaag
      121 aagccattcg tatgaacaac gtatcaggcg atcttttttt tatatgataa aaaatatcat
      181 taaaactttc ttctccttgc ggccaatcat ctgatggccg aaaagaaaca ggctctatca
      241 aatttctttt tagagaaact ctagtcaaag taatgttcct ttatggcaaa agataatttt
      301 tctactgatt tattaacatg ttgcagattc tcttttgttc gtttttgtga atctagcaat
      361 gaacgctttt ccttcgaaag tgaagatttt ttctcataca atcgctgctt ttgctcatca
      421 aatgcattgt ttttaatctg atatttatac aacgacactt tatccaaatt caccagcgat
      481 gcacaatatg cctgcttaga ggcaatcgac tctttaatgg attgctcgca gtcatcgagt
      541 ttaccatcca cctcttttat ctgagagtta atcttagcca ttgattctct caatcggttc
      601 gctctgcttt tacgaataga taaaattctg tccaacatac tcaggcaacc actttgaata
      661 ggctttcaat cgttttttcg taactactga tatctttggt gctttgttgt ataaagttct
      721 gaattaattt cctatttttt attgccttat ctgcttcagg atcttgcccc atcgtgtact
      781 caccaatacg aataagcagc tcaacatttt tataagttgc aataagcttt ttgcattctg
      841 ccgccgcacg caaatgctcc gatgtaacaa cgttatgcat aacacgactg gcacttaaac
      901 caatatcaat tgcagggaaa tggttttcct ctgcaagttc tcgggtaagt acgatgtgcc
      961 catcaagaat agagcggact tcatcgccaa taggatcatt aacattatcg ctttctaaca
     1021 acaccgtata aatcgcggtt attgaacctt ttggcgcagg ccccgctcgc tctaataatt
     1081 tgggtaacga agaaaaaaca ctcggaggga atccccccct tacatcaggt tctcccgatg
     1141 caagcccaac atcacgtgcg gctcgggcat aacgagttac agaatccatc attaataata
     1201 catttttacc ttgatcgcgg aagtactctg caatagtcgt cgccgtaaat gcggctttca
     1261 tcctttccag cgcggggcgg tctgacgttg tgacgaccag tacacattta gaaagcgtgg
     1321 attgaggtaa gagcgcgagg aattcattta cttcgcgacc acgttcgccg ataagagcaa
     1381 ggacaataat atctgcggat gcgccattac aaatcatccc caaaagcgta cttttaccaa
     1441 cgcctgaacc agcaaaaata ccgatacgct gcccaatacc gcaagttagt agcccatcaa
     1501 ttgccctgac gccaagtata aatggctgat caataacctg ccttaataaa ggatctggtg
     1561 gttcagcata caagctgcgt tcaaacggaa gatagggggc agcaatatta ctctccattg
     1621 gtctgcctat gccatcaaca agccgcccaa gtagtgcgtc tccaacacga atcttgaact
     1681 catctccctg ataacttaac cactgaccac agtacatgcc ggaaacatgc tcaaaaggca
     1741 acaagaaaac ctcatcttca tcaatagcga tcacctcggc taaacgttga ctgggttcta
     1801 tcttataaaa ggcgccaata cgcgcttttg gtaaacgcgc cttaataatc gtcccgccga
     1861 tattgataat cttgccttca tatctggtag acgaatttaa tgataatgcc ggcacagtgc
     1921 tattgagcac tttctgaata cgtgggtatt tttccaatac agaatcatgc tctgaaatca
     1981 tttaccgttc ctaatacttt aagttcgata ttattcccaa cctcctgaaa agagagcacg
     2041 gggactgacg ggaacgaacc ttcaatcatt ttcttcacat agcgcctgat atctaaagcg
     2101 gtcagaatga cgccgttccc cgttggattc atgatatttt ttaagaagcc aattatctgc
     2161 tcagttcgct ccggtgagat attcagatac gaacctgatg acgtttgtct gatcgactct
     2221 cgtagctcat tttcaatatc agagccaata agccaaacgt tcagaagtgt accgctaacg
     2281 ctatagcgac ctaaaatatg ccgacgcagg gcaatacgaa cgtattcgca caagataacc
     2341 acatcctttt cttttgttga ccaaaaaata agcgtctcaa agatagttct caggtctcta
     2401 attgagacat tttcctctac gagacgttgt aggatgtcaa caattttgct caaaccaagc
     2461 tggcgctgca gctctttcac aagctcgtta tattttctct ccatgatgtc catcaaataa
     2521 cgcgtttctt gtacgccgat gaactctttg gcattagaca atacaagttt ctttagcaaa
     2581 aacgttatct tatcatttgt tgttgcataa acatccatac ctgtaaggtg agcgagctgt
     2641 tgattagtct tattcgtctt atatacgata gagttagtcg aaagcttttc ctggacaaat
     2701 tcgaatgata tctctgcatg cccagcctca aaatagacag tatcatctat aagagtatct
     2761 ttgtatattg actcttgata gagcaaaaat tctattgcgc tatcattatt tttaaccgga
     2821 ttaacaataa tcttaggcaa tggaattcca ctttcctcaa agaaattcca acgcatgacc
     2881 tcaatttgct gtgtaatgtc tgaactataa atattactac ttaagtttaa aatcaatggc
     2941 tcagcgcccg gtgagatgtt ttggctatta gacaaatcag ctcccatagc atccttatta
     3001 ttgccagttc caaatgttct tttcttctgt aatttccagg ctatccccaa caggcaaacc
     3061 gccagaaata agaagaccaa tgcaggaaag cccggaataa tcgccattac gagcattagt
     3121 gcggcactga ttaaaaaagg gcgactatta acagaaacct gttgaacaat atcacctgca
     3181 aggttttctt ctttttcgct ttcaccgggc acacgagtaa caaccacgcc tgcggtgaca
     3241 gaaataataa gcgcagggat ctgggcaacc aaagcatcac cgacagacaa tacagaaaac
     3301 aggctaagtg ccgcactaaa tggcatgtca aattgccaca taccaatgag cacgccacca
     3361 aaaaggttaa ctaaaacaat aatgatacca gcaatagcgt ccccttttac aaacttcatc
     3421 gccccgtcca ttgaaccata aaactggctt tccttttgca ctcgggagcg aagaactttt
     3481 gcctcaagcg ggtcaataac gcctgcgcgc atatcaccat caatactcat ttgctttcct
     3541 ggcataccat caagggaaaa acgagcactc acttcagcaa cacgctcggc cccttttgta
     3601 ataaccataa attgaacaat ggtgataata gtaaaaataa ccaggccaac aacaatatta
     3661 ccgccaacaa caaagttacc gaaggagtaa ataatgtcac ccgcatcgtg atgcagcaga
     3721 ataagtctgg ttgtactaac actgagcgac aggcgcatca acgtcgtaat caacaggatt
     3781 gtggggaaag aagttagttc aagaggattt tttatataga ttgagagcat taataaaagt
     3841 aaagctgtcg aaatattaat cgcgataatg atatccacca caactgttgg tagtggaata
     3901 atcatcatca ttacagccat aaagaagaag agagccagaa taagatcgtg atatgactct
     3961 gcttttttaa atatatttaa gagtttattc atgatgtcat cctgcgaacg cgctcaataa
     4021 tctgcacatg ctgttgaaaa gtattcatta gaaaaaaaac atcgatattg gttaacaaac
     4081 aacttaagca caatttatta tcgtagacaa aaata
//
LOCUS       DABSYT020000206         4113 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.208, whole genome shotgun sequence.
ACCESSION   DABSYT020000206 DABSYT020000000
VERSION     DABSYT020000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.208"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            5..523
                     /locus_tag="ID725_RS24385"
                     /old_locus_tag="pgaptmp_004877"
     CDS             5..523
                     /locus_tag="ID725_RS24385"
                     /old_locus_tag="pgaptmp_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGV7853292.1"
                     /translation="MADNHTLSAGYAQSKVQDFKNIKGVNLQYRYEWDSPVSVVGSFS
                     YMKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYISFYGLVGISHTKAKGDY
                     EWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNVGYEGTKADIYGKHS
                     VNGFTVGVGYRF"
     gene            659..1450
                     /locus_tag="ID725_RS24390"
                     /old_locus_tag="pgaptmp_004878"
                     /pseudo
     CDS             659..1450
                     /locus_tag="ID725_RS24390"
                     /old_locus_tag="pgaptmp_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1455..2825)
                     /locus_tag="ID725_RS24395"
                     /old_locus_tag="pgaptmp_004879"
                     /pseudo
     CDS             complement(1455..2825)
                     /locus_tag="ID725_RS24395"
                     /old_locus_tag="pgaptmp_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2845..3192)
                     /gene="tnpB"
                     /locus_tag="ID725_RS24400"
                     /old_locus_tag="pgaptmp_004880"
     CDS             complement(2845..3192)
                     /gene="tnpB"
                     /locus_tag="ID725_RS24400"
                     /old_locus_tag="pgaptmp_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGV7853293.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3189..3863)
                     /gene="tnpA"
                     /locus_tag="ID725_RS24405"
                     /old_locus_tag="pgaptmp_004881"
     CDS             complement(3189..3863)
                     /gene="tnpA"
                     /locus_tag="ID725_RS24405"
                     /old_locus_tag="pgaptmp_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGV7853294.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT          926 a          988 c         1112 g         1087 t
ORIGIN      
        1 aagactggct gataatcata cgctatcggc tggttatgct cagagtaagg tacaggactt
       61 taagaatatt aaaggagtga atctgcaata tcgatatgag tgggattctc cggtcagtgt
      121 cgtcgggagt ttttcgtata tgaaaggtga ttgggcggac agtcatcgcg atgaagctga
      181 cgatttttac agacatcagg ctgatattaa atattattca tttctcgcag gcccagctta
      241 tcgtctgaat gactatatca gtttttatgg tttggtgggg atatctcata ctaaagcgaa
      301 gggagattat gagtggagaa acagtgtggg tgctgatgag tctgatggat atttaagtga
      361 gtctgtatca aaaaaatcca ctgattttgc ttatgcagct ggtgttatta ttaatccgtg
      421 gggcaatatg tctgtaaatg ttgggtatga gggaactaaa gcagatatat atggaaaaca
      481 ttcggtcaat ggatttactg ttggagttgg ttatcgtttt taatcattag acgggactgg
      541 ataattgcac ttgcgtagtg aagtttacta aatgtttgta ttttaatgaa gtattcgaca
      601 tattcccttc agacggaata tgtcgacggt tatggtttta ctggagcatg agtatgttat
      661 ggcgaaactg ataaatatat cagcgtccac tgaggagaga atgacgccag gggagaggag
      721 ggtggcttca cgactggaga gctttctgaa tgatgattgc tttgtctggt acgatatccc
      781 ggtgggacgg aaaaaccgac atccggattt tgtcattatt gacccggata acgggctggt
      841 atttctggag gtcaaggact ggactgtatc cacattacgt aaagcaaatc aggagcaggt
      901 tacactggaa accgacggtc ttctgaaatc agaaataaat ccgctggtac aggttcgtcg
      961 ttatgcctgt gatacggtta atgccttacc tgctgacccg tgcttacggc agaatgacgg
     1021 tcagtataag gggcggctga acctggcctg ggcgtatggt gtggtgttta cccgcattac
     1081 ccgtcagcaa ctgaaggcac tgacagggaa taatgagaat gcggtggaaa agatttttcc
     1141 ttcagcacag acaatctgtc aggatgaaat gacgcagtct gttttacctg aggtattccg
     1201 gcaaaaaatt gccgggatgt ttaccacggg gttcagaacc cgggtaacac cccggatgag
     1261 ggatattctg cgcgcccatc tgtttcctga agtgaccgtg aaacagaact cacaaatcaa
     1321 aattatggat attcagcagg agattctggc cagaaatatt ggtgacgggc atcgtgtcat
     1381 tcatggtgtg gccggttccg gaaaaaccat gattcttttg ttccgttgcc tttacctggc
     1441 agaaaccacg tccggtagtt acgccgcccc agtgcaacta cccggagggc gttttcacac
     1501 aggttattgt cgatctccac ccagccattg cggcagtatt cgttcagagc gtcccattgc
     1561 ttcagcagat aggtgaacgc tttcgctgta tccgagtggc gcgacagcgt gttcatctgc
     1621 ccctagagcc actcatacaa cgactgcatt agcggtaccg ttctggcttt tctgaccgcc
     1681 agtcgctctt ctgccggact gccgcggatc tcagcctcga tagcgtacag ttccccgata
     1741 cgacggagcg cttctcccgt tactgtcgtt ggatggcgga catgtacatc gtggatcttg
     1801 cgtcgggcat gtgccataca agccgcttcg gttgcctgac cgctttcgta aagagcattg
     1861 taacccgcat atgcatcggc ctgcaggata cctctgtagt ccgccagatg ttgctgtggg
     1921 tggatgcctt tgcggtcggg agagtatgag aaccacaccg ctgccggcat ggtcgaaccg
     1981 gcgttgcgat cgtcacgaac atagacccac agccgtccgg tacgggtttt accctgaccc
     2041 ggctccagta catttaccgg cgtgtcatcg gcatgcactt tacccggcat cagcacatag
     2101 tgcttcagtt catcatacag cgggcgaagc tgctctccca tgatgtcaac ccagcgcccc
     2161 atcgtattgc ggtgcagctc cacgccctgg cgggcataga tttccgactg acggtacagc
     2221 ggcagatgct cggcgaactt agccatgatt atgcgggcca gcagagccgg actggcgtaa
     2281 ctgcgctcga tgggttttgg tggctgcgga gcctgaacta tacagtcgca ccggctgcag
     2341 gcccgttttg ggcgaaccgt ttcgattacc ctgaacgcgg tgttgatgat atccagttgt
     2401 tcagagatgc tttctcccag cggtttcagt ttaccgctgc agacggggca ttcggtttct
     2461 gccggggaga taacctgcct gtcacgggga agtgttgccg gaagtgcttt gcggacggga
     2521 gagtctgatg ttttcggcgc tgtctctccg gccattgagg tgagttgcaa ctgcgcctca
     2581 ccaagcctgt tctggagctc ggttatacgc gtttctgccc gtgcgatctt cttttctatc
     2641 ttctcgcggc ttttctcgct gctgcgaccg aacaacattc tctgcagttt agcgaccagc
     2701 gctctgagtg agctgatctc gcgggcatag ccggttattt caccagacag acggacgata
     2761 acagcctgct gtgcgatcag cagggccttc agttgctcga tgttgtcggg gagtgtgttg
     2821 ttcattcccc tgttttatca cgggttatat ccggatgcca ggccgttctg tccgtttggg
     2881 atgttgccat gcgatcccct ccagtagcat ggataactga gctggcgtca ggtgcacttt
     2941 cccttcccgg gttaccggcc agacgaagcg gccccgttcc aggcgtttgg cgaacaggca
     3001 taacccgtca cgatcggccc acagtatttt caccattttg ccactgcggc cccggaagac
     3061 gaagatatgc ccggagaacg gggcatcttt cagcgtgttc tgcaccttcg aagccaggcc
     3121 gttgaagcca caacgcatat ctgtgatgcc agcgatgatc cagattctgg taccggttgg
     3181 cagcgttatc atcgggtacc tcctttcatt tcgcggatta gcgcccgtaa catttccgga
     3241 gtgagagggt caaacagttt taccacacct gatttaagat gcagctcgca ccgtgggacg
     3301 tttccgggat cactctcagg gcactcatca ggcttgttac gccagaaggg atttgtaact
     3361 ggtctggtcg gctctggcgt atcagtcaga gccaccggga caggcatgca ttcctgtatg
     3421 tcatcatcgc tcagtaagcc gtcctcgtac tggcttttcc attttaacag caggttatca
     3481 ttgataccgt gctctctggc gatccgggca acaacagcac cgggctgtaa tgcctgtttt
     3541 accagcctga ttttaaattc acgactgtag gaggttcgtc gttcttttcg ccatgtgcct
     3601 tcgctgattt gaggctctgt taattccttc tttctgttgg cataaaggat ggcgtcaagc
     3661 tgagctaatg aaactgaatc gggcaatggc catgcgatac cggatgcaat aaatcgctga
     3721 aaaagcgtat gtattgtgga atgactgaga cctagacgct gagcgatggc ccggatggtc
     3781 agtttatctt caaatcttaa acgcagagca tcaggcaaat aagaacggaa gcagggaata
     3841 tctttttttg tctgggaatt catcgttcgt gtccatctat atagatgggc gcgattgttg
     3901 ccagacagga caattttcac aagacgtcgc agatggggcg cttacacaac aatggcgcga
     3961 gggaaagctg ctattacctt cttcagagag cccccagcta cttcctgtga ctctcgatgc
     4021 agctgccgaa cagccagaat cgctcgcaga ggacccggaa accctcagta tcagctgttg
     4081 tcaacgacgg atgactattg caccgttcgt gac
//
LOCUS       DABSYT020000207         4081 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.209, whole genome shotgun sequence.
ACCESSION   DABSYT020000207 DABSYT020000000
VERSION     DABSYT020000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.209"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..925
                     /locus_tag="ID725_RS24410"
                     /old_locus_tag="pgaptmp_004882"
     CDS             <1..925
                     /locus_tag="ID725_RS24410"
                     /old_locus_tag="pgaptmp_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGV7853295.1"
                     /translation="YDDKHKIEGFMYLKENDDANDINPALPPGRHLKIGTFKFESKGT
                     LRGQRFLKKAFDHAFSSKSDDIYVTVFDKHVHLIKLFQTYGFYIHGEKETHNGKEFVY
                     ARSLHEPYGDILLDYPRIMTSRANKYLLAIYPEYHTRLFPDSKLVNESPDIVKDISHA
                     NSIHKIYICGMRSVMGMKRGDIIVIYRTGDKKGPARYRSVASTLCVVESVKNISEFLS
                     EDSFVDYCIRFSVFSEDELRKIYKERRYPFIIRFTYNLSLPKRPNRAILIDHVGLNGS
                     RAFRWSHFKLTNEQFLKIIELGKINESFIIH"
     gene            903..1280
                     /locus_tag="ID725_RS24415"
                     /old_locus_tag="pgaptmp_004883"
     CDS             903..1280
                     /locus_tag="ID725_RS24415"
                     /old_locus_tag="pgaptmp_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGV7853296.1"
                     /translation="MKVLLSIKPEFAEKILNGTKRFEFRKGIFKNPQISTVVIYATMP
                     LGKVVGQFRIESILSDEPESLWKKTEKHAGISKQFYDSYYSGREKAYAIKIGEVERYK
                     EPIPISALGSNIKPPQSYLYLPA"
     gene            1761..1967
                     /locus_tag="ID725_RS24420"
                     /old_locus_tag="pgaptmp_004884"
     CDS             1761..1967
                     /locus_tag="ID725_RS24420"
                     /old_locus_tag="pgaptmp_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HGV7853297.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            2043..2339
                     /locus_tag="ID725_RS24425"
                     /old_locus_tag="pgaptmp_004885"
     CDS             2043..2339
                     /locus_tag="ID725_RS24425"
                     /old_locus_tag="pgaptmp_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGV7853298.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            2345..3130
                     /gene="bet"
                     /locus_tag="ID725_RS24430"
                     /old_locus_tag="pgaptmp_004886"
     CDS             2345..3130
                     /gene="bet"
                     /locus_tag="ID725_RS24430"
                     /old_locus_tag="pgaptmp_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGV7853299.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            3127..3804
                     /locus_tag="ID725_RS24435"
                     /old_locus_tag="pgaptmp_004887"
     CDS             3127..3804
                     /locus_tag="ID725_RS24435"
                     /old_locus_tag="pgaptmp_004887"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGV7853300.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            3804..3986
                     /locus_tag="ID725_RS24440"
                     /old_locus_tag="pgaptmp_004888"
     CDS             3804..3986
                     /locus_tag="ID725_RS24440"
                     /old_locus_tag="pgaptmp_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGV7853301.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            3959..>4081
                     /locus_tag="ID725_RS24445"
                     /old_locus_tag="pgaptmp_004889"
     CDS             3959..>4081
                     /locus_tag="ID725_RS24445"
                     /old_locus_tag="pgaptmp_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGV7853302.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLA"
BASE COUNT         1210 a          937 c          931 g         1003 t
ORIGIN      
        1 ctatgatgac aagcataaaa tcgaaggttt tatgtatcta aaagaaaatg atgatgcaaa
       61 tgacattaat ccagcgctcc caccaggacg tcatctaaag ataggaacat tcaaatttga
      121 atctaaaggc acccttcgcg gacaacgatt tctaaaaaaa gcgtttgacc atgcattttc
      181 atcaaaatct gatgatattt atgttactgt tttcgacaaa cacgtccatc taataaaact
      241 tttccaaacg tacggatttt acattcatgg tgaaaaagaa acacataacg ggaaagagtt
      301 tgtatatgcg aggtctttgc atgagcctta tggtgatatt ttattagatt accctcgaat
      361 aatgacatca agagccaaca aatatttact ggcgatttat cccgaatatc acactagact
      421 attccctgat tcaaaacttg taaatgaatc accagatatt gtcaaagata tatcccatgc
      481 taacagcatt cataaaattt acatatgtgg aatgcgttct gtgatgggaa tgaaaagagg
      541 agatatcatt gtcatctata gaaccggaga caaaaaaggg ccagctcgct atcgttctgt
      601 agccagtaca ttatgtgtag ttgagagcgt aaaaaatatt tctgaatttt taagcgaaga
      661 tagttttgta gactattgta ttcgttttag cgtattttct gaagatgaac tcagaaaaat
      721 ctataaagaa cgtcgatacc ctttcattat aagattcaca tacaatctgt ctttgccaaa
      781 gagacccaat cgtgctattt taatagatca tgtggggcta aatggttcgc gtgcattccg
      841 atggagtcac tttaaactca caaatgagca gttcttaaag atcatcgagt taggcaagat
      901 aaatgaaagt tttattatcc attaagcctg agtttgcaga aaaaatattg aacggaacaa
      961 agcggttcga gtttcgtaaa ggtatattca aaaatccgca aattagcacc gttgttattt
     1021 atgccacgat gccattaggt aaagttgttg gtcaattccg tattgaatca atactaagtg
     1081 acgaaccgga atctctttgg aaaaagacgg aaaaacacgc aggtatttct aagcaatttt
     1141 atgactcata ttattcaggt agagaaaagg cctacgcaat aaaaattggt gaagtggaaa
     1201 gatataaaga accaattcct atctctgctc taggtagtaa tattaagcca ccacaatcat
     1261 atctttacct acctgcgtaa gaatcccggc caccgtgccg ggttttcttt tgcccctccc
     1321 cctcatcaca caaaccattc aaaaaaccac cacgacctcg cttcagttat cgctatgcga
     1381 tgcaagtcac aaaataaatc catcctaaat acaaccattt acatctaaaa aaaccaataa
     1441 aacaactttt gttgttgacg acaaaacaac tatagtttta aataaattca tcgcaacaac
     1501 acaacgatac ggcaaccacc tgattcaccg ttgcgatgac cgcttagatc cgcagcttga
     1561 atttcagcag gctccgggaa gtgcgagggg tgaagtggac gcgtgaacgt cggtgtgacc
     1621 agctgaaatc aactcaacac ctcatacctc agtcgcttca acgaggcggc ttagttatga
     1681 caaccggcgg ccatccaccg cctgaatacg cgcagaagtc tctatatgtt cagcagccca
     1741 gcttacgggc aggagttttt atggttcatc aacattacgg aacgcagacc gttaatcgcg
     1801 gtgcggtcat gccaggaatg ctggtcaaac acaaagatgg tacctggact gcatcagcta
     1861 atttacgcgg acggctttat ctgcatcgcg gcatcgagcg cacttatacc cgtgatttgc
     1921 tcgtggaagt ttttctcgac ggacgcggca acggcctgaa tcactaatcc cctttcctgt
     1981 ttacctaatc agcccggcat ttcgcgggcg atatattcac agccattttc aggagttcag
     2041 ccatgaacgc ttattacatt caggatcgtc ttgaggctca gagctgggcg cgtcattacc
     2101 agcagattgc ccgtgaagag aaagaggcag aactggcaga cgacatggaa aaaggtcttc
     2161 cacagcacct gtttgaatca ctctgcatcg atcatttgca acgccacggg gccagcaaaa
     2221 aagccattac ccgtgcgttt gatgacgatg ttgagtttca ggagcgcatg gcagaacaca
     2281 tccggtacat ggttgaaacc attgctcacc accaggttga tattgattca gaggtataaa
     2341 acggatgagt acagcactcg caacgctggc agggaagctg gctgaacgtg tcggcatgga
     2401 ttctgtcgac ccacaggaac tgatcaccac tcttcgccag acggcattta aaggtgatgc
     2461 cagcgatgcg cagttcatcg cattgttgat cgtcgccaac cagtacggcc ttaatccgtg
     2521 gacgaaagaa atttacgcct tccctgataa gcagaacggc atcgttccgg tggtgggcgt
     2581 tgatggctgg tcccgcatca tcaacgaaaa ccagcagttt gatggcatgg actttgagca
     2641 ggacaatgaa tcctgcacat gccggattta ccgcaaggac cgtaatcatc cgatctgcgt
     2701 taccgagtgg atggatgaat gccgccgcga accattcaaa acccgcgaag gcagagaaat
     2761 cacggggccg tggcagtcgc atcccaaacg gatgttacgt cataaagcca tgattcagtg
     2821 tgcccgtctc gccttcggat ttgctggtat ctatgacaag gatgaagccg agcgcattgt
     2881 cgaaaatacc gcatacactg cagaacgtca gccggaacgc gacatcactc cggttaacga
     2941 tgaaaccatg caggaaatta acactctgct gatcgccctg gataaaacat gggatgacga
     3001 cttattgccg ctctgttccc agatatttcg ccgcgacatt cgtgcatcgt cagaactgac
     3061 acaggccgaa gcagtaaaag ctcttggatt cctgaaacaa aaagccactg aacagaaggt
     3121 ggcagcatga caccggacat tatcctgcag cgtactggga tcgacgtgag agctgtcgaa
     3181 cagggagatg atgcgtggca caaattacgg ctcggcgtca tcacagcttc agaaattcac
     3241 aacgtaatag caaaaccccg atcaggaaag aagtggcctg acatgaaaat gtcctacttc
     3301 cacaccctgc tggctgaggt ttgcaccggt gtggctccgg aagttaacgc taaggctctg
     3361 gcctggggaa aacagtacga gaacgacgcc agaaccctct ttgagttcac ttccggcgtg
     3421 aatgttactg aatccccgat catctatcgc gacgaaagta tgcgcaccgc ctgctctccc
     3481 gatggtttat gcagtgacgg caacggcctt gagctgaaat gcccgtttac ctcccgggat
     3541 ttcatgaagt tccggctcgg tggtttcgag gccataaaat cggcttacat ggcccaggtg
     3601 cagtacagca tgtgggtgac acgaaaagat gcctggtact ttgccaacta tgacccgcgc
     3661 atgaagcgtg aaggcctgca ttatgtcgtg attgagcgga atgaaaagta catggcgagt
     3721 tttgacgaga tggtgccgga gttcatcgaa aaaatggacg aggcactggc tgaaattggt
     3781 tttgtatatg gggagcaatg gtaatgaagc atcctcacga taatatccgg gtaggcacga
     3841 tcactttcgt ctactccgtt acaaagcgag gctgggtatt tcccggcctt tctgttatcc
     3901 gaaatccact gaaagcacag cggctggctg aagagataaa taataaacga ggggctgtat
     3961 gcacaaagca tctcctgttg aattaagaac gagtatcggg atggcacata gcctcgctca
     4021 aattggagtc aggtttgtgc caataccagt agaaacagac gaagaatttc atacgttagc
     4081 c
//
LOCUS       DABSYT020000208         4011 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.210, whole genome shotgun sequence.
ACCESSION   DABSYT020000208 DABSYT020000000
VERSION     DABSYT020000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.210"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..173
                     /locus_tag="ID725_RS24450"
                     /old_locus_tag="pgaptmp_004890"
     CDS             <1..173
                     /locus_tag="ID725_RS24450"
                     /old_locus_tag="pgaptmp_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGV7853303.1"
                     /translation="TLKNESLSHYRFKSRDEAISVIREYIEIFYNRQRRHSRLGNISP
                     AAFREKYHQMAA"
     gene            124..825
                     /gene="traT"
                     /locus_tag="ID725_RS24455"
                     /old_locus_tag="pgaptmp_004891"
     CDS             124..825
                     /gene="traT"
                     /locus_tag="ID725_RS24455"
                     /old_locus_tag="pgaptmp_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HGV7853304.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(873..1124)
                     /locus_tag="ID725_RS24460"
                     /old_locus_tag="pgaptmp_004892"
     CDS             complement(873..1124)
                     /locus_tag="ID725_RS24460"
                     /old_locus_tag="pgaptmp_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGV7853305.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1148..1438)
                     /locus_tag="ID725_RS24465"
                     /old_locus_tag="pgaptmp_004893"
     CDS             complement(1148..1438)
                     /locus_tag="ID725_RS24465"
                     /old_locus_tag="pgaptmp_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGV7853306.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            2124..2483
                     /locus_tag="ID725_RS24470"
                     /old_locus_tag="pgaptmp_004894"
     CDS             2124..2483
                     /locus_tag="ID725_RS24470"
                     /old_locus_tag="pgaptmp_004894"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGV7853307.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            complement(2576..>4011)
                     /locus_tag="ID725_RS24475"
                     /old_locus_tag="pgaptmp_004895"
     CDS             complement(2576..>4011)
                     /locus_tag="ID725_RS24475"
                     /old_locus_tag="pgaptmp_004895"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGV7853308.1"
                     /translation="TPFSFRYIFKPFFCILFICSSIVTYATMKYGVQFDKTMMQNIFE
                     TNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGITYRLISMFASLLII
                     FAIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFKYVRDFFVSPGEFRQTGTDASRT
                     INEKQKPVIMFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAIS
                     VPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEP
                     SNSDNTLCKKNSCYDEVMLKNIDEYINNNSEDKLIVFHLMGSHGPTYYLRYPESHKYF
                     KPTCDRSDIENCTHEQLINTYDNTIRYTDYIISKLIDKLIEYKDEYDTVLLYVSDHGE
                     SLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDN
                     IFSSLLGLWNVNTSVYNKEDDIISSCR"
BASE COUNT         1267 a          822 c          757 g         1165 t
ORIGIN      
        1 gaacgctgaa aaatgagagc ctgagccact atcgttttaa gagccgggat gaggccatct
       61 cagtaatacg ggaatacatt gagattttct acaatcgtca gcgtcgtcac tctcgtctgg
      121 ggaatatctc cccggcagcc ttcagggaaa aatatcatca gatggctgct taaaaaaaga
      181 acaaatggta gtgtccgcta ttgtcagtac acctcaacca tctggcttga acctgcgagc
      241 gaacgcaccg ttttcctgca gatcaaaaac acgtcggata aagacatgag tggactacag
      301 ggaaaaattg ccgatgcagt caaagccaga ggttatcaga tcatgacttc cccggataaa
      361 gcttactact ggatccaggc taacgttctc aaagcagaca aaatggatct gcgtgaatct
      421 cagggatggc tgagtcgcgg ttatgaaggt gcagcagtcg gagctgctct tggaggtgga
      481 atcacagcgt acaataccgg gtcggcaggt accactctcg gtgttggact ggctaccggt
      541 ctgattggca tggccgcgga tgcaatggtg gaagatataa attacacaat gattacagat
      601 gttcagattg ccgagcgaac cagaaccagc gtcagaacgg ataatgttgc agctttgcgt
      661 cagggaacat caggctccaa aattcagaca agcacagaaa ccggcaacca gcataaatac
      721 cagacccggg ttgtatccag tgccaaccag gttaatctga agtttgaaga agctaaacct
      781 catctggaag accagctggc aaaatcaatc gccaatatac tctgatataa atatcccggt
      841 ggccattcac ctggcctccg gccctcttag tctcaacgtt cagacaattc agatattatt
      901 ttccctgtca caccatatac ccatgccgga acagaatcta atgacggtac cagtaaattc
      961 ttcagcgttc ggtttgcctg acgcccgtag ctgtatttgt gtctgtttct tcttatacaa
     1021 gagcagtagt acaatttctc ttccagagtc agttcaactt taggacgtaa ccaataaaga
     1081 tctcttccac tataaaatgg tgaatcctgt acaaacgatt ccataacaga acctcctgct
     1141 gctacagtta acacagctgc tggcatggac taataccatc ttttaattta accctggcgg
     1201 agacgccatt atttttggac gttcttgaaa caaaattaat tcctgaagaa tctttctcca
     1261 gtttattcag ctcaagattc agtccataat taacgtcaaa taactctgat aaaggtatca
     1321 gtttatgttc aggataatga ttatcccgac gacaacaaat attaaaatcc tgcatattac
     1381 ccgacatcag gagttcaaaa ccgtccccgg catcattcat actggcagat aaattcatta
     1441 cccagtttag tgctaatcaa caggtacggc ttaacttaca ggaattcccc gggaggggcg
     1501 gactcatcct cagttagcgg caaaatcgca tcacagccaa tcgcctgtct cgattcccca
     1561 tcggcgaaca atgtatgtgg ccacaaaatc ctggctggct cagaacgaag agcatctgag
     1621 caaagaggtc atcaatctgg tatgaatatc tggcagaaaa tatcccctga atccacaact
     1681 gctgtgcata tctgctcaaa tcagcatcaa tcataaacca gtaatcacag tgcctgtgat
     1741 tgggttacag gaataagtcc cccttcataa ggcatatcat caataccggt gaaggaaaag
     1801 aaagcatcag atggcctgat agtctgcgtc agtcaatgac atcagtgctt tatccggaac
     1861 aatgtatcca tgttggcgac agagaaagcc attgccatga acagttttgt ttatccccgg
     1921 aaagtcattt ctgttacaaa tttatattga caaacttgct ttcgatgaga aacatactat
     1981 ttcaatgaaa tgcagaatga ggcaaaattt cgttatgttt tttaattccc gaaagaataa
     2041 atgtggctta ttacaaaatc ctgttaatgt taactgatat tttataatgc atattcagac
     2101 atataattaa ataagaggta tttatggcta atacgaccac tggtgttacc agaattatta
     2161 aagcagcggg ttattcatgg aaaggcttat gtgcggcatg gcgctatgaa gcagcctttc
     2221 gtcaggagtg cattctggtt ctgtcagcac tgataatatc cttttggctg gatgttgact
     2281 ctgttactcg agtattactc attggttcgg caatgatggt aattattgtt gaaataatta
     2341 acagtgcaat tgaagcagtt gttgacagaa ttggttcaga acatcacgaa ttatcaggca
     2401 gagcaaaaga tatgggttca gccgcagtgt tgattacgat tttagttgct atcattatat
     2461 ggggagtttt attactttca taacgcccaa catctaaatt attcacagtt acctggttaa
     2521 tatttactta aggataagag gtattcacct cttatcctca gaaaaatgtc agtatctatc
     2581 tgcaggagga tataatatca tcttctttat tataaactga agtatttaca ttccataacc
     2641 ccagtaacga tgaaaatata ttatcatgac ttactgttct gtttagagca tgttctgaca
     2701 aacaattcaa atcaattttt ttagaggaaa cgaaccccgg agacatccag gtaataagag
     2761 gcacatgcgt ttgctcagct ggagcaacat tatatggtgt tccatgtaag tataatccat
     2821 tttctcccag agattctcca tggtcagaga catataataa aacagtatca tactcatctt
     2881 tatattcgat aagcttgtca attaattttg atattatata atcagtatag cgaattgtat
     2941 tatcataggt attaatcaat tgctcatgcg tacaattttc gatatcacta cgatcacatg
     3001 ttggtttaaa atatttatgt gactcaggat aacggagata gtaggtagga ccatgactac
     3061 ccatcaaatg aaatacgata agtttatcct ctgagttatt atttatatac tcatcaatat
     3121 tttttagcat aacctcatca taacatgagt ttttcttgca taatgtatta tctgaatttg
     3181 atggctcaat attaattgta gggattcgct tacatacacc tttacatccc ccgtcatttt
     3241 caatccagag taaatttacc cccgttctgt acagaatatc cagtacattt tccgaattta
     3301 ccgctttgcg ggaattgaac tcttttcgct tcatatctga aaacatgcat ggaacagata
     3361 tggctgtgga tgtgccacac gactgcacat tatgaaagga tataagttca ttatatttct
     3421 ttgtaaaatc attggtacca cgagaatatc cattcagagc atagttttga cttcttgctg
     3481 tctccccgac aactaaaaac ataattaccg gcttttgttt ttcatttatt gtgcgggatg
     3541 catccgttcc agtctgacga aactcgccag gagaaacaaa aaaatccctt acatatttaa
     3601 acccactgta gatataatta gtaggtatta tttctttatt taatgaagag ttgtttctgc
     3661 caactgatgc ataatcctta taaaagaaaa acgcaatcgc aaatataatc aagagagatg
     3721 caaacattga tattaaacga tatgtaatac cctttatcca tgtctcagga taacggatat
     3781 ttactaacaa caatagtcca catggaagta tacccgtaaa taaaaaccac agtacaacag
     3841 acatattgaa atatgatgtc atttcacctg catttgtttc aaaaatattc tgcatcattg
     3901 ttttatcaaa ctgcacacca tatttcatgg tggcatatgt aacaatggaa ctacagataa
     3961 acagaataca aaaaaaaggt ttaaatatat acctgaatga gaacggtgta a
//
LOCUS       DABSYT020000209         4005 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.211, whole genome shotgun sequence.
ACCESSION   DABSYT020000209 DABSYT020000000
VERSION     DABSYT020000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.211"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..385)
                     /locus_tag="ID725_RS24480"
                     /old_locus_tag="pgaptmp_004896"
     CDS             complement(<1..385)
                     /locus_tag="ID725_RS24480"
                     /old_locus_tag="pgaptmp_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGV7853309.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKLTGGEGAVISATRDPEYYFIVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGVAC"
     gene            625..768
                     /locus_tag="ID725_RS24485"
                     /old_locus_tag="pgaptmp_004897"
                     /pseudo
     CDS             625..768
                     /locus_tag="ID725_RS24485"
                     /old_locus_tag="pgaptmp_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(897..973)
                     /locus_tag="ID725_RS24490"
                     /old_locus_tag="pgaptmp_004898"
     tRNA            complement(897..973)
                     /locus_tag="ID725_RS24490"
                     /old_locus_tag="pgaptmp_004898"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(937..939),aa:Arg,seq:tct)
     gene            complement(987..1063)
                     /locus_tag="ID725_RS24495"
                     /old_locus_tag="pgaptmp_004899"
     tRNA            complement(987..1063)
                     /locus_tag="ID725_RS24495"
                     /old_locus_tag="pgaptmp_004899"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1027..1029),aa:Arg,seq:tcg)
     gene            complement(1073..1148)
                     /locus_tag="ID725_RS24500"
                     /old_locus_tag="pgaptmp_004900"
     tRNA            complement(1073..1148)
                     /locus_tag="ID725_RS24500"
                     /old_locus_tag="pgaptmp_004900"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1113..1115),aa:Ile,seq:cat)
     gene            complement(1189..1314)
                     /locus_tag="ID725_RS24505"
                     /old_locus_tag="pgaptmp_004901"
                     /pseudo
     CDS             complement(1189..1314)
                     /locus_tag="ID725_RS24505"
                     /old_locus_tag="pgaptmp_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(1593..2027)
                     /locus_tag="ID725_RS24510"
                     /old_locus_tag="pgaptmp_004902"
     CDS             complement(1593..2027)
                     /locus_tag="ID725_RS24510"
                     /old_locus_tag="pgaptmp_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGV7853310.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(2113..2253)
                     /locus_tag="ID725_RS24515"
                     /old_locus_tag="pgaptmp_004903"
     CDS             complement(2113..2253)
                     /locus_tag="ID725_RS24515"
                     /old_locus_tag="pgaptmp_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGV7853311.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2250..2612)
                     /gene="rusA"
                     /locus_tag="ID725_RS24520"
                     /old_locus_tag="pgaptmp_004904"
     CDS             complement(2250..2612)
                     /gene="rusA"
                     /locus_tag="ID725_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGV7853312.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2609..2899)
                     /locus_tag="ID725_RS24525"
                     /old_locus_tag="pgaptmp_004905"
     CDS             complement(2609..2899)
                     /locus_tag="ID725_RS24525"
                     /old_locus_tag="pgaptmp_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGV7853313.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2892..3062)
                     /locus_tag="ID725_RS24530"
                     /old_locus_tag="pgaptmp_004906"
     CDS             complement(2892..3062)
                     /locus_tag="ID725_RS24530"
                     /old_locus_tag="pgaptmp_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGV7853314.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3062..3517)
                     /gene="ybcN"
                     /locus_tag="ID725_RS24535"
                     /old_locus_tag="pgaptmp_004907"
     CDS             complement(3062..3517)
                     /gene="ybcN"
                     /locus_tag="ID725_RS24535"
                     /old_locus_tag="pgaptmp_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HGV7853315.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQQLRDKQEA"
     gene            complement(3514..3615)
                     /locus_tag="ID725_RS24540"
                     /old_locus_tag="pgaptmp_004908"
     CDS             complement(3514..3615)
                     /locus_tag="ID725_RS24540"
                     /old_locus_tag="pgaptmp_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853316.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            complement(3732..>4005)
                     /locus_tag="ID725_RS24545"
                     /old_locus_tag="pgaptmp_004909"
     CDS             complement(3732..>4005)
                     /locus_tag="ID725_RS24545"
                     /old_locus_tag="pgaptmp_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7853317.1"
                     /translation="GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNAL
                     SAIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
BASE COUNT         1016 a         1055 c          905 g         1029 t
ORIGIN      
        1 tacaggcgac accgcccggt gtcaccgtac tgcggcgcgc cagctgctta atacgcgggt
       61 ccggacggtc atatgtctcc ggcagcggaa ggccttcacc atatgccatg ctgtttgact
      121 gccccgccag aaccacaata aagtaatact ccgggtctct ggtggcgctg attactgcac
      181 cttctccacc tgtcagcttc accacaacag gtgtgctcac atcaccttct gcgacaatcg
      241 cctgaataag tgctgcgcca tcatccgtat acgaagaaaa cggcccaccg tatggttgcc
      301 atccttcacg aattttttgc gcaagtgcat cagcaaggtc tgacggcgac gccgccctga
      361 caacatcata atgtttaaat gtcattattc ctcccggccg ggatagtgta ttaaatcaga
      421 tatggagtgg gctgtagtcc ggaagcctga atgacacacg gggactacag cccaagaaat
      481 gaaaaaaggc cacgcagttg cgcagcctga taaaccctgg ttaaaatcca cacgataaca
      541 acacaacaat atcagtatct catgctattg cccgaaccca ttcgggcatt ttttacccat
      601 aaaaaagccc ctccggagag gggcatgttt gcatgcacat tctttttctt gcatggtgcc
      661 gggtgcctcc cggtgaattc agtatcagca cctgaatccg cgattatcac atatacctgg
      721 ttgctgattg cccctccgca cagggggatt caccatgcga aattttttta acaaacgctc
      781 agcatgtcag gcaacattca actgcctgaa ttgtgaggca tttaacattt cactgtccgg
      841 tgtctttcct gtaataaaaa gcccgcaaaa gagagtcagg gcagataagt gtggtgtggc
      901 gcgttgtact ggattcggac cagtgaccga ttgcttagaa ggcaattgct ctgtccggct
      961 gagctaacaa cgctgaatac cgataatgga ccgccatcgg ggacccgaac cccgcgcaac
     1021 cagcttcgaa ggctggcgct ctgtcctgat gagctaatgg cggtatgtga tatggtggcc
     1081 cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct
     1141 aaagggccgg gcgcaggata ataacggtac gtaactaatc ctgcaatatc atccgttctg
     1201 actgactaaa tcctgaactt ccctgaccgt ctgctcaaaa cgttcagtct ccagctcaac
     1261 gccaattgca cgacgcccca gcgacattgc tgctttgacg ctacggacat aaaaaagcca
     1321 gccactgggg gaggctggca aactcgtaga gcaaaatgct gttacgcaaa cttcgttaca
     1381 gggtcatcct gcaatacaaa aaatacacaa tatttagaaa actaatagtg ccatgtgcaa
     1441 tttttaagat tttgttatta attgtggtcg caccttcctt tctgtgtact ttccgtatag
     1501 ctcacaggat tctgggtaca aaaaaacccg cgcatcggcg ggttaataag cagcgtggca
     1561 atgtaaccac tcttatcatg atatgcagat ttttacgatc gtaaactatt ttttcgctga
     1621 taaaatacag aggttctccc tcccggcaat tcacgctcaa cataccgatc catctcaagc
     1681 ctcactccca gcatcatcag catgccttca acaatcccct ccgctttgtg aaggcgttta
     1741 cctatacagg tgtcagagca cccatgtttc cgtgccagcg ccatgaacgt ctcccccaac
     1801 acgtaataat caaccagcaa gtcatgcaga tcgcgattgt tccggtaaag gcgggctata
     1861 cacccgcata tcaccatcgc atcatcgtca cagcactgcg ggcgtgattt tactttttcg
     1921 gggatcagtc ccttaaaacc ggcggcaatg gacgaccagg tcacatcttc atgattatta
     1981 gccgcccacg ctccccaacg ctcaagaacc atctgaatat cacgcatcaa cttactccac
     2041 aaaaatcaga ccagaacgcc aattacaagc aaaaatcaac aaaacagtat tagttgattg
     2101 ttatctctga cttcatactc ctgctcctgt cagggttttg gcgtaattct tcagtattcg
     2161 gtaatcggtc aaaacagaac cggggaaacg atataagcgc agacgccccc agcggtggcg
     2221 aagaagttct gccatattaa actcaaacat cattcattcc ccatttcggt gatggtcagt
     2281 tccagcctcc cacctttggt aacaggcatc ttcacaacgc ggtaatcaac gacctgagca
     2341 tcatccagcc agaaacctgc tttagtgagt gcgtcaaaag cggctttttg cagattatcc
     2401 aggtcacggc gacggcgatc cggcatgtgg cactcaatgc ggattttcac aggcatagcc
     2461 aggccgatat ccagcattgc gtttttaatg attcgggcga cgttatcgcg gtatgcctgc
     2521 ccctctgcac tgacgtgcgt gcgcccgcga ttatggcggt aatagcgatt attgctcgga
     2581 ggccagggta atgtgatact gtaggtattc acgccttaat aaccccctct ttcagccaga
     2641 taacctgtgt tctcgccata ccttccagcg cgcattcttt tgcatatgca gcatcgacaa
     2701 aatgtgtgcg gcggtcgatt tcgtcgtggc aggcagaaca tgcaatggtg gcaatcaggt
     2761 ctggcggttt gataccggta ccgcacaatc cagccagccg gatatgtgcc agtacagacg
     2821 tttcagaatt gccattacat acgccaggga ttcttacctg gcattcccga ccacgcgctg
     2881 cttttctcaa atcagccatg attcctcctt gctgccagtc gcaaccattt tttatcaacc
     2941 aggctagcgg tatatccgag cagtgttggt atttcggatg gcttcagctc aggcttacgc
     3001 ttacgacgat ttgatactct gtagatgtgt ccgttcatga cacgaataag cggtgtagcc
     3061 attacgcctc ctgcttgtca cggagctgct ggaactcgca gctctgcgga atagtcagat
     3121 ggcaaccaat attcatcgcc caggcttcaa ccttacacag gaagacatac atctctccgg
     3181 tatcaagatc ggaggtatgg cgtaacgact ggatagtggt gatatcaccg gttacgacat
     3241 caaccaggtc tttggtttca taaccgagat atgtgtgttt gagagcatct tttacccaag
     3301 ctggagtggc gaacgtttta cccctgctga tgaggtattc actgatttcg ctgtaccaca
     3361 tgtggctgag tgcattctgg gaaagactgc gtttctcacg ccacggttta agcaccatgc
     3421 gaaagcattt gccctcctcc agataaggct ggatctgccg accgatagcg gtgaagttgc
     3481 cgcgatgtaa tttgatgccg tcttgtgaga ggttcacgct tcacctccgc agaggtcaaa
     3541 cgctagatgc aaagaattgc aggtgcattt ctgcatctgt gaagggagaa gagagtttgg
     3601 attgtatgtg cgcataaacg tccccgttta gcgcagaagt caccggagtt gttcaggctc
     3661 cggtgacata attatgccgt gttgatttcc caaaatcaaa atcgatagaa ttgctccttc
     3721 ttaaaacact tttactctct ggaagctttt cttatctctc ttggtgttat attaaaacga
     3781 ttatgaaatc tttcagtaaa acgcgaagga cacttataac cattttctct ggcaatctcg
     3841 cttataggtt ttaccgtcgt ttgtatagca gacaacgcat tatttaacct cacatcgtcc
     3901 agtatacttt ggaaacttac cccctcgctt gctagacggc gatgtaatgt agaaacagaa
     3961 atgtagagat atcgagcaac cttgtttgct gtccattttg tgccg
//
LOCUS       DABSYT020000210         3879 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.212, whole genome shotgun sequence.
ACCESSION   DABSYT020000210 DABSYT020000000
VERSION     DABSYT020000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.212"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..827)
                     /locus_tag="ID725_RS24550"
                     /old_locus_tag="pgaptmp_004910"
     CDS             complement(<1..827)
                     /locus_tag="ID725_RS24550"
                     /old_locus_tag="pgaptmp_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGV7853318.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLG"
     gene            complement(883..1215)
                     /locus_tag="ID725_RS24555"
                     /old_locus_tag="pgaptmp_004911"
     CDS             complement(883..1215)
                     /locus_tag="ID725_RS24555"
                     /old_locus_tag="pgaptmp_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGV7853319.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(1225..2544)
                     /locus_tag="ID725_RS24560"
                     /old_locus_tag="pgaptmp_004912"
     CDS             complement(1225..2544)
                     /locus_tag="ID725_RS24560"
                     /old_locus_tag="pgaptmp_004912"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGV7853320.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(2525..>3879)
                     /locus_tag="ID725_RS24565"
                     /old_locus_tag="pgaptmp_004913"
     CDS             complement(2525..>3879)
                     /locus_tag="ID725_RS24565"
                     /old_locus_tag="pgaptmp_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGV7853321.1"
                     /translation="SFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCID
                     VERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSRLFRTQFRMVSPKRISNPNNTSD
                     SRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDG
                     QTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSQ
                     EQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLL
                     RYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLE
                     EAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTY
                     EKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA
                     "
BASE COUNT          768 a         1208 c         1061 g          842 t
ORIGIN      
        1 ttccccagca ccatcgtgtt gtccggcagg aagttctttt tgacgccgtt ttccacgtac
       61 tgtccggaat acacgacgat ggccacatcg ccatacattc ccttataaga caccgctttg
      121 cccaggtctt ttaccgctgt ctccagttcg gaatgagagc cgcgacgggt atccagcttc
      181 tccctgacgg ctttgaagga acggaacagc gcccagcctt tcgggtcaaa cacgatgata
      241 ttcaccacgc cgctggcgtt cagcgcgtag gcttcgatat cgtcggtcgg gtcatacgtg
      301 gacttgtcac gcttgctcca ctccgtgccg ccggactgcg tgatgttgtt cgccgcactg
      361 cggcccatat ccacctcaac cggatcgaag gcttcaccgg tcatggtgta tttgcccttg
      421 agcacggcag aaactgcctg catctcttcg acctgagcaa tggccagctc ttcgtcacgc
      481 atgttctgca tgatgatgcg acggcggcgg taagccgggt ccgccagatt ctgcggatct
      541 tcatccggca ggcgacgcag ggtcatctgc ggattcactt catgcttcgg cttgacatat
      601 cccggcgtaa attcagaggt ggagccgcca cgggaacgga taacctcacc agaaacaatc
      661 ggcgaaacgt acagcgccat gtttaccagt cccggaattt gtgagagata gactttctcc
      721 gtggtgaagg gatagctctc acggaaaaag agacgcagaa acagcggatc aaacttaaat
      781 ttctgctcat ttgccgccag cagttgggcg gttgtgtaca tcgacataaa aaaatcccgt
      841 aaaaaaagcc gcacaggcgg cctttagtga tgaagggtca ggttaaacga tgctgattgc
      901 cgttccggca aacgcggtcc gttttttcgt ctcgtcgctg gcagcctccg gccagagcac
      961 atcctcataa cggaacgtgc cggacttgta gaacgtcagc gtggtgctgg tctggtcagc
     1021 agcaaccgca agaatgccaa cggcagcacc gtcggtggtg ccatcccacg caaccagctt
     1081 acggctggag gtgtccagca tcagcggggt cattgcaggc gctttcgcac tcaatccgcc
     1141 gggcgcggtt gcggtatgag ccgggtcact gttgcccagc ggctggtaat gggtaaaggt
     1201 ttctttgctc gtcataaaca tcccttacac tggtgtgttc agcaaatcgt taacggcatc
     1261 agatgccggg ttacctgcag ccagcggtgc cggtgccccc tgcatcagac gatccagcgc
     1321 agtatcactg cgcgcctgtg cactctgtgg tgctgcggcc agaatgcggc gggccgtttc
     1381 cacggtcata ccgggggttt ctgccagcac gcgggcctgt tcttcgcgtc cgtgagcctc
     1441 ctcacagttg aggatcccca taatgcggct gttttctgcc gcaaccgcag cggtgatctg
     1501 cgcgttcacg tccggctgcg ccgcgctggc gttttcgccc tccgtcgctg gcaccacgcc
     1561 agtaacgtca gcctgcgaag cagtggctga aacagttgtt gattgagtct ctttggtcat
     1621 tcgccctcct gagagacggg atttacgtgc atccagtgca tcacgcataa cggtgatcgc
     1681 atcggtgctg ttgacaagtt catcagccag tccggcatca atggcctcct gaccgctgta
     1741 cactgcagcc tcggtatcca gcacagcctg cacggacagg ccggtatatg ccgacacctt
     1801 ctgcgcaaac atccggcggg ttgcatccat ccgggactgc agtgtttccc ggacatcacc
     1861 cggtagatgg ctgtaggggt tgccatccac cttatggctg ccgctgtaaa tcagcgtgat
     1921 ttccacgccc tgtttctcca gggcagcacc gtaattactg tgagccatca tgacgccgat
     1981 ggagcctgtc cgggcggtct gcgtgaccag acgccgggag gcggcgctgg caagcagctg
     2041 acctgcactg cagttcatgt cgttggccag cgcccatacc ggttttatgt ctcgcacacg
     2101 ggcgatgatg tcagcacagt caaatgctcc cgccaccatc ccgcccggtg tgtccatatc
     2161 gagcagaatg ccgtccacca tcggatcgct ggcagcctgt tgcagacggg cgataatgcc
     2221 gttgtaaccg gtcattcccg aatacggctg cagcgcccgc gtccggctga ccagcgtacc
     2281 ggacaccggc agcacggcga tgccgttcat gacctgataa ctccgggcct gtcgtggtcc
     2341 gtcatcatca ccggataacg ccagcgccgc gggtgcctct ccggcagtca ggctgtcgcc
     2401 ggatactgca tccgtcaggc ggctgatccc aagctggcct gcaagcgcac aaaagaaaac
     2461 ccgcgcatag gcgggttcaa gcatcagcgg ctcattaaaa gccatgctgg caatatgcgg
     2521 gagattacgc agctctgctg tcactcttct cctcctctgt tgattgtcgc agcccggatt
     2581 caaatgccgc agccgcccag gcgggcggtt taagaccagc tgcgcggcgc tccatcgttt
     2641 cacggacctg ctgggcaaaa atttcctgat agtcgtcacc gcgtttcgcg cactctttct
     2701 cgtaggtgct cagtccggct tctatcagca tcaccgcttc ctgaacttct ttcagaccat
     2761 cgatggccat acgaccggag cctatccagt cgcagttccc ccaggcactg cgggcttcct
     2821 gaaagctgaa gcgcgctttt gaaggtaacg tcaccacgcg gcgaacgatg gcctcttcca
     2881 gccagcacag aaacatctgg ctcgcctgac gggatgcgac gaattttcgc cgccccataa
     2941 agtacgccca cgactcgttc gcactggccc gtgccgtgga gtagctcatc tgggcgtaat
     3001 tccgggaaag ctgctcatac gagacaccca gcccggcagc gatataccgc agcagtgact
     3061 gctcaaacac ggagtagccg ttatccgtgt cctgagccgt ctgcaggttc agtgagtccc
     3121 ccggcatcag gtgcggcact tttgcgcctc ccagacggac cggtgctgcg gcgtaatacg
     3181 cggcaatttc accaatccag ccggtcagcc tttcccgctg ctcctgactg ttcgcgccca
     3241 gaataaaatc catcgctgac tgcgtatcca gctcactctc aatggtggcg gcatacatcg
     3301 ccttcacaat ggcgctctgc agctgcgtgt tctgcagcgt gtcgagcatc ttcatctgct
     3361 ccatcacgct gtaaaacaca tttgcaccgc gggtctgccc gtcctccacg ggttcaaaga
     3421 cgtgaatgaa cgaagcacga ccgccgggta actcgcgggg tatccatgtc catttctgcg
     3481 gcatccagcc aggatacccg tcctcgctga cgtaatatcc cagcgccgca ccgctgtcat
     3541 taatctgcac accggcacgg cagttccggc tgtcgctggt attgttcggg ttgctgatgc
     3601 gcttcgggct gaccatccgg aactgtgtcc ggaacagtcg cgagggacgg gtatcccagg
     3661 tggcctgaac gaacagttca ccgttaaagg cgtgcatggc cacaccttcc cgaatcatca
     3721 tggtaaacgt gcgttttcgc tcaacgtcaa tgcagcaaca gtcatcttcg gcaaactctt
     3781 tccatgccgc ttcaacctcg cgggaaaagg cacgggcttc ttcctccccg atgcccagat
     3841 agcgccagct tgggcgatga ctgagccgga aaaaagacc
//
LOCUS       DABSYT020000211         3873 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.213, whole genome shotgun sequence.
ACCESSION   DABSYT020000211 DABSYT020000000
VERSION     DABSYT020000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.213"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            293..1030
                     /locus_tag="ID725_RS24570"
                     /old_locus_tag="pgaptmp_004914"
     CDS             293..1030
                     /locus_tag="ID725_RS24570"
                     /old_locus_tag="pgaptmp_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HGV7853322.1"
                     /translation="MDLKNKYFGKTKEEAAKKKIDIKKENKQIFDAVKQFERNEIATV
                     QGKAKFITRIAVVLLISNIALAAAIAFLSPLKTAVPFVIRVDNNTGYTDIAPQLSGAK
                     QTYQEAETKYNLAKFVINYESYDWQTIQEMLDTVNVMSNNKVFSQYNTAIRADNSPLN
                     VLKDSYKIKTKIKSVTLLKPDVAQVRFSKMILDSSGSLAPEYRVTDWIATVAFDFNKD
                     VKTEEQRLINPLGFEAVSYRVDAEAIK"
     gene            1027..1806
                     /locus_tag="ID725_RS24575"
                     /old_locus_tag="pgaptmp_004915"
     CDS             1027..1806
                     /locus_tag="ID725_RS24575"
                     /old_locus_tag="pgaptmp_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HGV7853323.1"
                     /translation="MIKKSGLIGCLSLLAVCASPATIAATYGQGSAFDSRIQSVVFNE
                     NDVVNVRVKKGAVSVVQIAKDETIKDVGLGDPAAWKVSVREHTVFFRPAVEDNPSTNV
                     TIVTDKHTYSLYLTEVSSNPTYVLRYDYPKPVTAKVFTEKKYPCTDGGVINGHYQLKG
                     SKSIFPYQVWDDGTFTCMRWNSKQDLPVPYRVDADGNENLVNKHMENNTMVLHEVSDN
                     FRLRIGDQVVDVRTSSTVPKMWNKKGTTNGKVRVEKFNYEK"
     gene            1796..2938
                     /locus_tag="ID725_RS24580"
                     /old_locus_tag="pgaptmp_004916"
     CDS             1796..2938
                     /locus_tag="ID725_RS24580"
                     /old_locus_tag="pgaptmp_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HGV7853324.1"
                     /translation="MKNESRSNNDVGNRGVIEAKSKKSSKKIFILLASIFGIFVVAAM
                     IIKMLPSKEVEQTGLEPKNETLISNSQDGASLNSVMKTIMQNKQREETERKQREEQAE
                     KEKKQKTEINSDNDSTMQAMAMQGQSSSHSSSQSGNGQEKERPLTKVERQITGDTLVK
                     IETRNEDKRSEKEKDSLQGETYADGTVSFASNRRFLLAAGTSLSCVLQTKIVTSYPGI
                     TLCQLTKDLYSDNGETLLARRGAMLMGEQNKVMTQGVARVFVNWTNLKDGNANVKIGA
                     LGTDGLGASGLPAWIDNHIFQRYSGAIMLSLLGDGMDILKNTTQKSGNNSNITYDNTS
                     DTAQELAKTTLENTINIPPTAYINQGTVLSVIVPRNIDFSEVYGVQ"
     gene            2938..>3873
                     /gene="virB11"
                     /locus_tag="ID725_RS24585"
                     /old_locus_tag="pgaptmp_004917"
     CDS             2938..>3873
                     /gene="virB11"
                     /locus_tag="ID725_RS24585"
                     /old_locus_tag="pgaptmp_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HGV7853325.1"
                     /translation="MSEEHIIARQFLVQTGIQELLDMDDVTEIAVNQPGRIWFEGRDG
                     WCFRDTDKVSYKALMGLAYALCNISGLEDALKPHNPIASVVLPGGERGQIIVPPATED
                     NCVVLSIRKPSLTRFSLDDYVNSGRFDRVKIAKKHTAVLTERQKYLFELSRKGDAASI
                     AKFIREAVNDRLNFLIVGGTGSGKTTVGKAIADLFPPERRYATIEDVHEMTLPNHPNH
                     IHLFYKKGIVDAKSIIEACMRLKPDHIFLAELRGDEAWSYLEALNTGHEGSISTIHAN
                     NTYASFSRLASIVKQSSVGMTVDMDLIMRTIKTSID"
BASE COUNT         1203 a          681 c          907 g         1082 t
ORIGIN      
        1 gcaaaaatgg atttgtacgg ttttgatgac tttcttcctg ttatatcagg aacgacggaa
       61 gggattgttc tgtgtgaaca agtaagggaa gaacttggta cagatgaacc cgatatctgg
      121 attcctgaat ttattaaacg tattaaggag caaaagaaat gagagcaact attgcactct
      181 tattaatctg ttttctttct tcttgttcat caccgcctca acctaaacaa gcggagggcg
      241 gatggtatga attgaataca acaattcagg atgtgaaagc gaggacgatg taatggattt
      301 aaaaaataaa tattttggta agacaaaaga agaagcagct aagaaaaaaa ttgatataaa
      361 gaaagaaaac aagcaaattt tcgatgctgt caagcagttt gagcgtaatg aaatagcaac
      421 tgtccagggt aaagccaagt ttattacgcg aattgctgtt gtgctgttga tttccaatat
      481 tgctcttgca gcagccatcg cttttttgtc tccgttaaag actgctgttc cttttgttat
      541 tcgtgtggat aacaatacgg gctatacaga tattgcaccg caattatcag gagcaaagca
      601 aacctatcag gaagcggaaa caaaatataa tcttgcgaag tttgttatta actatgagtc
      661 atacgactgg cagactatcc aggaaatgct ggatacggta aatgtcatgt ctaataacaa
      721 agtgttctca cagtacaata cagctattcg tgcagataac tcgccgctga atgtactgaa
      781 agacagttat aagattaaaa cgaaaattaa gtccgtcact ttactgaagc ctgatgttgc
      841 gcaggtcaga ttctcaaaaa tgattctgga ctcttccggc tctttggctc ccgaatacag
      901 agttacagac tggattgcaa cggtcgcttt tgattttaat aaagatgtta agacggaaga
      961 acaacggctt attaatcctc tcggatttga agctgtcagc tatcgtgtag acgcggaggc
     1021 tattaaatga ttaaaaagtc gggtcttatc ggttgcttat ctttactggc tgtttgtgca
     1081 tctccggcaa caattgcggc aacatatggt caggggtcag catttgactc caggattcag
     1141 agtgttgttt ttaatgaaaa cgatgttgtt aatgtcagag taaagaaagg tgctgtttcg
     1201 gtagttcaaa tcgccaaaga tgaaacgatt aaagatgttg gcctgggtga tcctgctgca
     1261 tggaaggtgt ccgttcgtga acatacagtc ttctttcgtc cggcagtaga agacaatcca
     1321 agcacgaacg ttactattgt aacggataaa cacacgtact cattgtacct tacggaagtg
     1381 tccagtaatc cgacatacgt tttgcgatat gactatccta aacctgtaac agccaaagta
     1441 tttacggaga aaaaatatcc ctgcactgat ggtggtgtaa ttaatggaca ctatcaatta
     1501 aaggggagta agagcatttt cccttaccag gtctgggacg atggtacttt tacctgtatg
     1561 cgctggaaca gtaaacagga tttacccgtg ccttatcgtg ttgatgctga tggcaatgaa
     1621 aaccttgtta ataagcatat ggaaaataac acaatggtat tacatgaagt gtctgataat
     1681 ttccgcctga gaataggcga tcaagttgtt gatgtaagaa catcttcgac ggttcctaaa
     1741 atgtggaata aaaaaggtac tacaaatgga aaggtgaggg tggagaaatt taattatgaa
     1801 aaatgaaagc cgcagcaata atgatgtggg taatcgtggt gttatcgagg ctaaatcaaa
     1861 gaaatcctcc aagaagatat ttatcttgct ggcaagtatc tttggtattt ttgttgtcgc
     1921 tgccatgatt attaaaatgc ttccctcgaa ggaggtggag caaactggcc ttgaaccaaa
     1981 aaacgagacg ttaatatcta attctcagga tggggcaagc cttaattctg tgatgaaaac
     2041 aatcatgcag aataagcagc gtgaggaaac tgaacgcaag caacgggaag aacaggcaga
     2101 aaaagaaaag aaacaaaaaa cagaaataaa ttctgataat gatagcacta tgcaagcaat
     2161 ggcgatgcag gggcaatcca gcagccattc ttcatcgcag tctggcaatg gacaggagaa
     2221 agaaaggcca ctgactaagg ttgaacgtca gattacagga gatactctgg ttaaaattga
     2281 aaccagaaat gaggataaac gttcggaaaa agagaaggat tctctgcaag gggaaactta
     2341 tgctgatggt acagtgagtt ttgccagtaa cagacgtttt ttgctggctg cgggtacttc
     2401 gctttcatgc gttttacaaa caaaaatagt tacgtcctac cctggaataa cgttgtgtca
     2461 actgactaaa gatctctatt cagataatgg tgaaacatta ctggctcgcc gtggcgctat
     2521 gctgatggga gagcagaata aagtaatgac gcaaggagtg gctcgtgtat ttgttaactg
     2581 gactaattta aaagatggca atgctaacgt aaaaattggt gctcttggca ctgatggact
     2641 tggtgcatct ggcctgccag cctggattga taatcatata ttccagagat attcaggtgc
     2701 gattatgttg tcattgcttg gtgatggtat ggatattctg aaaaacacca cacaaaaatc
     2761 ggggaataac agcaatatta cctatgataa tacatctgat acggcgcagg agttggctaa
     2821 aacaacactt gagaacacga ttaatattcc tccaactgca tatattaatc agggaactgt
     2881 tttatcagtg attgttccga ggaatattga cttttctgag gtttacggag tgcagtaatg
     2941 agtgaagaac atattatcgc tcgtcaattt ctggtgcaaa cagggattca ggaattactg
     3001 gatatggatg atgttaccga aatcgccgta aatcagcctg gcaggatatg gtttgaaggt
     3061 cgtgatggtt ggtgctttcg tgatacagat aaggtcagtt ataaggcatt aatggggctg
     3121 gcctatgcgc tgtgtaacat aagtgggctg gaagatgccc tgaaaccaca taacccgatt
     3181 gcgtcggttg ttcttcctgg tggtgaacgt ggccagatta tcgttcctcc tgcaaccgag
     3241 gataattgtg ttgttctctc aattcgtaaa ccatcactaa cgcgtttttc acttgatgat
     3301 tacgttaatt cagggcgttt tgaccgagtt aaaattgcta aaaaacacac agcagtgtta
     3361 actgagcggc agaaatattt attcgagtta tcgcgtaagg gtgatgccgc aagtattgct
     3421 aaatttattc gtgaggcggt caatgatcgc ctgaatttcc tgattgttgg tggtactggt
     3481 tccggcaaaa caacagtagg taaagccatt gcggatttgt ttcctcctga acggcgctat
     3541 gcaacaattg aagatgtcca cgagatgacg ctcccgaatc atccaaacca tattcacctg
     3601 ttttataaaa agggaattgt ggatgccaag tcgattattg aagcctgtat gcgccttaaa
     3661 ccagatcata tctttctggc tgagttaagg ggggatgaag catggagtta tcttgaagct
     3721 cttaacacag ggcatgaagg ctcaataagt acgatccatg ctaacaatac ttatgcgtcg
     3781 ttctcgcggc tggcgagcat tgttaaacaa tcatctgttg gtatgacagt tgatatggat
     3841 ttgattatga gaacgattaa gacatctata gat
//
LOCUS       DABSYT020000212         3831 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.214, whole genome shotgun sequence.
ACCESSION   DABSYT020000212 DABSYT020000000
VERSION     DABSYT020000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.214"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..898)
                     /locus_tag="ID725_RS24590"
                     /old_locus_tag="pgaptmp_004918"
     CDS             complement(<1..898)
                     /locus_tag="ID725_RS24590"
                     /old_locus_tag="pgaptmp_004918"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HGV7853326.1"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTT
                     LLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHA
                     LKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
                     IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLE
                     ELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPV
                     DIGWSDVGSWQSLWDISLKSKTG"
     gene            complement(880..1389)
                     /locus_tag="ID725_RS24595"
                     /old_locus_tag="pgaptmp_004919"
     CDS             complement(880..1389)
                     /locus_tag="ID725_RS24595"
                     /old_locus_tag="pgaptmp_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGV7853327.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
                     KDIICLMRQ"
     gene            complement(1392..2357)
                     /locus_tag="ID725_RS24600"
                     /old_locus_tag="pgaptmp_004920"
     CDS             complement(1392..2357)
                     /locus_tag="ID725_RS24600"
                     /old_locus_tag="pgaptmp_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HGV7853328.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMA
                     SASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDTTK
                     PDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            complement(2360..3478)
                     /gene="gmd"
                     /locus_tag="ID725_RS24605"
                     /old_locus_tag="pgaptmp_004921"
     CDS             complement(2360..3478)
                     /gene="gmd"
                     /locus_tag="ID725_RS24605"
                     /old_locus_tag="pgaptmp_004921"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGV7853329.1"
                     /translation="MTKVALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERI
                     DHIYQDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSF
                     VGKGIEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWR
                     PEITLAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
     gene            complement(3498..>3831)
                     /locus_tag="ID725_RS24610"
                     /old_locus_tag="pgaptmp_004922"
     CDS             complement(3498..>3831)
                     /locus_tag="ID725_RS24610"
                     /old_locus_tag="pgaptmp_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HGV7853330.1"
                     /translation="EQIELIKNCIAVIQPTLFEGGPGGGVTFDAIALGKKVILSDIDV
                     NKEVNCGDVYFFQAKNHYSLNDAMVKADESKIFYEPTTLIELGLKRRNACADFLLDVV
                     KQEIESRS"
BASE COUNT         1184 a          798 c          657 g         1192 t
ORIGIN      
        1 ctcctgtttt cgattttaga ctaatgtccc ataacgattg ccaagatcca acgtcactcc
       61 aaccaatatc aacagggcat acaacacatt tttctgtttt ttccattaca gcaaaatcaa
      121 tagattcagc aggacaatct tgaaattgtt cttttgataa tcgaataaaa tctagatcaa
      181 tgtatgagga tgaggcaacc tgttcacaaa cattgtaaat gtcaggtcta aattttctca
      241 attcctcaag ataaacagat gccttaaaca tgaatattcc actattccaa tagtgattac
      301 cagaagtcat atacaattct gcggtttcac gattaggctt ttcgacaaac ttatttacat
      361 aataaaaacc agtattttca tgcccttgaa gcggtacaga tagttcacct ctctcaatat
      421 acccataacc agtttcagca tattctggta taattccaaa cgttacaatt ttaccttgat
      481 tagcgatggg agttgcattt ttaatagcat cacagaaaac actttcttta gctataacgt
      541 ggtctgccgc aagaactaga agcaatggat cttcctgagg attacgtttt aacgcatgaa
      601 acgcagatat cgctattgct ggtgcagtat ttcgcccgca tggttctaga ataatattac
      661 catttaattt atttatttcc cttaactgtt cagccacaac aaagcgatgc tgttcatttg
      721 ttatcactaa tggtttttga catgataggc ctgaaagtcg tagcaaagtc gtttgtaaca
      781 aggtgttatc accagagagt tgtaaaaact gctttggata tagttcacga gaaagtggcc
      841 aaagacgact gcctgtacca ccggccataa ctacagcaat tattggcgca tcagacatat
      901 tatatcctta ttatctaagc caatcgcatc atttgtttta ttgttaaaat atgcgcgaca
      961 attataatgc acatcgtcat catttatcag ctttgctcgc gatagccagc aataagcatt
     1021 atgttgtgac ttcggtaatt tcaaaatgtt tggttgtaat ttaagaagga atgctataac
     1081 tatataatgc gttgaaaagt ctccccccat actattgtct tcgtagaagt gctgccagat
     1141 accataaaac ttacccacag agagaggcaa acgaattcct agttcaattt ctgtcaatcg
     1201 ttcaaaggct gtctgcaatt tttcatcttt caacacccta ccaccaggaa cgaaccaata
     1261 gccctgtgcc gggcggttga ttcgttttcc tagcaaaatt tcgccaaact cgttttccac
     1321 aatcaaatct atagaaataa gaggagtaga ccttacaatt gtggcaaagt cttgggaatg
     1381 taaaaacatt attacccccg atattgaagt tggttttcaa gaaaccagtt gtatgtattt
     1441 tcaagaccct tgtgaagggt aattttatga ttccaaccta gttgatgaag aagcgttaca
     1501 tcaagtagtt ttcgaggggc tccatcgggc tttgttgtat cgaacgtaat atgcccttta
     1561 taacctacaa cttttgctat tgtttccgca agctcacaaa tcgtgcagtc aatacctgtt
     1621 ccaatattga tatgagacaa cattacttta gtattttttt gccatatatc gtatggcatc
     1681 tccatgacat aaatgcttgc agaagccata tcatctacat gtaagaattc acgctttgga
     1741 gtaccacttc cccaaacaac aacattcgga gaattgtttt ccacagcatc atgaaagcgg
     1801 cgcaaaagcg ccggaatcac atgagaatta cttggatgaa aattgtcatt tggaccataa
     1861 agattggttg gcattactga acggtaatca cgcccaaact gacggttata agattcacat
     1921 aatttaatac ctgcaatttt tgcgatagca taaggttcat ttgttggctc aagtttccct
     1981 tgtaataatt cgtcttccat aatcggttgg tgtgctaact taggataaat acacgacgaa
     2041 ccgaggaaaa gcagtttatt tacattattt ttgtgggcag catgaatgac attcgcctct
     2101 atcattatat tctcatatat aaaatcggca ggataagaac tgttagctaa aatacctccg
     2161 acttttgctg ctgccaaata aacctggtcg attttctgtg aagaaaaaaa atccaaaaca
     2221 gcgctactat ccaacaagtt caattcatcc cgagtacgta aaaccaactc aacatcatca
     2281 cgttgtttga ggcgtcgggt aatagctgat ccaaccattc cttggtgacc agcaataaaa
     2341 atacgttgct tattcatcat cattccagag ctaagcttac agaaaaacca tgcgatttta
     2401 aaagagaatg ttttttagcg gcttcaagat ctttggcaac catttcagaa atcatttcag
     2461 caagagtaat ttctggtctc caaccaagtt tgagattagc tttgctcgga tctccaagca
     2521 aagtatcaac ttcagctggt cggaaataac gaggatcaac agcaacaatg acatcacctg
     2581 gtttcacacc tggagcatcc tgtccttcaa ccgaatctac aatgcctttt tcttcgattc
     2641 ctttaccaac aaagctcatc ttaataccaa gttgtgctgc tgccatttcg acaaactgac
     2701 ggactgagta ttggactcct gttgcaatca caaaatcttc gggttgctcc tgttgtaaca
     2761 tcaaccattg cattctaaca taatcttttg catgtcccca atctcgtaac gaatccatat
     2821 tccctaaata caaacatgat tccaagcctt gtgcaatatt tgcaagtcct cgagtaattt
     2881 tccttgttac aaacgtttct ccacggcgtg gagattcatg attgaacaat ataccattac
     2941 atgcataaat accatatgac tctcgataat ttaccgtgat ccaatatgcg taaagttttg
     3001 caactgcata aggggaacga ggataaaaag gggtggattc tttttgaggg atttcctgaa
     3061 caagtccata taattctgag gttgaagctt gatagaaacg cgttttgttt tccaatccta
     3121 aaaagcgaat tgcttccagt aaacgtaatg taccaattgc atcgacatcg gctgtatatt
     3181 ctggagactc aaaagaaact gctacgtgac tcatagcagc taaattatat acttcatctg
     3241 gctgtacctc ctttagaatt ctagtgaggt tagatgaatc agtcagatct ccatagtgca
     3301 agtgaaaatt tgggttagaa ccatgtggat cttgataaat atggtctatg cgttctgtat
     3361 taaaagatga ggctcggcgt ttgataccat gaacttcata ccctttatca agcaaaaact
     3421 cagctagata agatccatct tgtccagtta cacctgtaat aagagcgact ttagtcatta
     3481 tatacctctt gaatatatta agatcgggat tcaatttctt gtttcacaac atctaaaaga
     3541 aaatctgcac acgcattgcg tcttttgaga cccaattcta tcagagttgt aggttcataa
     3601 aaaattttag attcatcagc ttttaccatc gcgtcattta atgaataatg gttttttgcc
     3661 tgaaagaaat atacatcacc gcaattaact tctttattga catctatgtc agatagtata
     3721 acttttttcc ctaatgcaat agcgtcaaat gttacccccc ctccaggccc gccttcaaat
     3781 aaggttggtt gtattacagc aatgcaattt ttgattaatt caatttgttc a
//
LOCUS       DABSYT020000213         3786 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.215, whole genome shotgun sequence.
ACCESSION   DABSYT020000213 DABSYT020000000
VERSION     DABSYT020000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.215"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            517..1860
                     /locus_tag="ID725_RS24615"
                     /old_locus_tag="pgaptmp_004923"
     CDS             517..1860
                     /locus_tag="ID725_RS24615"
                     /old_locus_tag="pgaptmp_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853331.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(2009..3517)
                     /gene="putP"
                     /locus_tag="ID725_RS24620"
                     /old_locus_tag="pgaptmp_004924"
     CDS             complement(2009..3517)
                     /gene="putP"
                     /locus_tag="ID725_RS24620"
                     /old_locus_tag="pgaptmp_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGV7853332.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
BASE COUNT         1117 a         1012 c          853 g          804 t
ORIGIN      
        1 tttttctact atgacattat cacaaaccca ctagcatgaa tacagaaaga gaagtaataa
       61 gatgagacgc cactgctttc agatgaaccc tgtaattagg gtcgagaata taatttaccc
      121 attcccctaa cgataaatta aaaaatttgc gagttaattt ttctctggct gaaggctaac
      181 gtcgagttat cgataaaatt cacgagtaat tgtaactata tactttatga aacacaggaa
      241 gaaattatta tttaacgcga tgataaaagg cgtaaaaaaa aataataatc ttcatgaaac
      301 tcaccccgat ggcagcattg taaattcgcc aataactggc attcctcctg ctttttacgg
      361 gtgtttccat taacaccaga atgagcggat tgtttactac atcaagaacg catccgaagt
      421 gtacatcctg gtgcgccgac tgaatcgccc ggagccatgc ggatggctac gacttaatac
      481 gaatcattaa ccctgtaagg atacggagag cattacatga gaccaatatc caacctcaat
      541 acgagcgcag caacctatat tccaccgcaa caactcccga gtagttgtct ggaaactttg
      601 acgcttctgg tgcagatgaa taattatatc aaaagggatg cggactacag cacgggcatg
      661 gctgtagctc cactggttcc tgaagaggtt cacttgctgg ctgctgcgat gacaaccgaa
      721 ctgcatcgac atcagttcca accggtggta agtgcaaatg atctgcaact tcctgaaccg
      781 ttcaccttcg atgttgccgg tttcaccatc accttcacga agacgcaaga gcatgacaac
      841 acgggcaatc tcatgaaaat tgcagtcagc aagagcggtg tgtgcaccag tacaaatatt
      901 actcttgagc tgtttcactc cattgtcaca accctgatgt cacgcagtca atatggaacc
      961 tttgatctct ggtccgtacg gccaatactc acggatgaaa gtcaaaacag ggtgcatgaa
     1021 gcagcccgct actccccggc ccagcaatac ggaagagaag aaacacactt taccaacaat
     1081 gggatgcgag aatttggaaa tatgtctccg ctggcgtggc gtaacgatgt ggaattacag
     1141 caatcgtgta atacaagcaa catcccacca aatgctgcaa atgacgacat aaacaacacc
     1201 aggcagacca tcgaggacca gccagaggcg gacgagccac agcaatatgt taaattgact
     1261 gtagacgaca tgcgaaaatg ggcggctatg gaccagcagg cgcgaaacgc tctacaggga
     1321 gtttcaggat ggtgtacgcg caatcatttt aatatcaaaa atgccagaaa ttacctgacc
     1381 gatcacggcc tgaactacgc gggacaggta aaggtaaaca ggccgcatga atatgccaaa
     1441 ttcacccttg aacatatccg ccagtgggca gccttaaaca agcatgtgcg aaaaccggtt
     1501 ggttatttag aaaagtggtg taaggaaaga aacctcgcgc caactactgc caggaattac
     1561 ctgaagaacg acgggcttac cgccctgggt gagttgaagc tgacagggcc gcagaaatgg
     1621 gtcacgttca ccttcggaga catcgtacaa tgggcgaata tgacccagga ggagcgtaac
     1681 agcgcagggg gagccgaaaa gtggagtaaa aaacgcggct tccagtggtc tactgccaga
     1741 tcctacctga aatcgagcgg agtgaccaag caaggtgcga gaaaactggc gtggttaaaa
     1801 aacagcggga atatgtcgaa tccattctat ctcgcccaac caaccagccg caggacataa
     1861 gctgaaggta ccgatagcgg caattttgtt catctgtgcc gggtgggcaa atagcaattg
     1921 cccccgaaca ctccctgcca ggcaacttat accgcatggc agggaacatt cgggttaaaa
     1981 agcaaaagat aactaattac tcttcagctt agccttcctg caaccgtgac ggcggagccg
     2041 aatgatagtg cgcatcggcc tcggcaaagc gtttttgcat cgccgctgac ggtgctttac
     2101 ccagcaaact aaacactaca atcccaatac tgccaaagat aaagcccgga ataatttcgt
     2161 acagtcccag ccagccgaac tgtttccaga cgatcaccgt cagcgcaccg atgatcatcc
     2221 ccgccagcgc accgttgcgc gtcatgcgtg accacatcac cgaaaacagc accaccggac
     2281 caaacgccgc gccaaagcct gcccacgcgt agctcactaa gcccagcacg cggttttccg
     2341 ggttcgctgc caacgcaatc gccaccagcg ccaccaccag caccatcaca cgccctaccc
     2401 acaccagttc tttctggctg gcatgtttac gcagaaacgc tttgtacaaa tcttcggtaa
     2461 tcgcactgga acataccagc agctggcaac ttaacgttga cattaccgcc gccagaatcg
     2521 ccgacagcag aatcccggca atccacgggt taaacagaat ttgcgccagt tcgataaaca
     2581 cgcgctcggc gttctggttt accgcaccag ctaccgacgg gtgctcgtta aagtaagcga
     2641 taccaaagaa gccgacagcc accgcccctg ccaggcagag gatcatccag gtcatactga
     2701 tacgacgcgc atggacgatg ctgtggtgag aatccgccgc cataaaacgc gccaggatgt
     2761 gcggttgccc gaagtaaccc agcccccacc ccatcagcga gataatggcg acaaagttca
     2821 gccctttgag catatccacg ttttcgatgc tcttttgttt gatcacttcc agcgagtcac
     2881 caaagccacc gacactgata atgacgataa ccggcgtcag gatcagggca aaaatcatca
     2941 ggctggcctg tacagtgtca gtccagctca ccgcgaggaa accgccaata aaggtgtaaa
     3001 ggatcgtcgc cgcagctccc gcccacagag ccgtttcgta gctcatgcca aaggtacttt
     3061 caaacagacg cgcgcctgcc acaatgcccg aagcgcaata aatggtgaag aacagcaaaa
     3121 tcaccagcgc ggagataatg cgcaaaatgc ggcttttatc ttcaaaacgc ccggtgaaat
     3181 aatccggtaa tgttaaggcg ttattgttgt attcggtatg cacacgcaac cgcccggcca
     3241 ccagcttcca gttaatccac gcgcctaatg tcaggccaat ggcgatccaa ctttcggaaa
     3301 tcccggaaag aaaaaccgcg cccggcaacc ccattaacag ccagccgctc atatccgatg
     3361 cacccgccga taatgccgtc acgaatggcc caaggctacg tccgcccaga atatagtcgt
     3421 caaagttttt cgttgatcgc caggcgataa acccaatcaa tatcatgcca aagatataga
     3481 cacaaaatgt caccaacatc ggtgtgctaa tagccatcta aagtctccaa aaaattatta
     3541 tcggcaatgt cgaaacttgc cgttatatct gccaccggaa cggggtaaca gagtttatgt
     3601 tttaccaggg cgaccgtatc ctgccggaag cgctggttat tcacaatcga tttaacacac
     3661 catttacatt aaattttagt gctcagcgac actatttttc atcaggttgc actctctcac
     3721 attttttgcg gttgcacctt tcaaaaatgt taactgccgc agagaaaaag tctgagttat
     3781 tttttt
//
LOCUS       DABSYT020000214         3778 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.216, whole genome shotgun sequence.
ACCESSION   DABSYT020000214 DABSYT020000000
VERSION     DABSYT020000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.216"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            65..292
                     /locus_tag="ID725_RS24625"
                     /old_locus_tag="pgaptmp_004925"
     CDS             65..292
                     /locus_tag="ID725_RS24625"
                     /old_locus_tag="pgaptmp_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853333.1"
                     /translation="MACNVFAFSNSGGGSETSYKEPEKTSAMTTTHSTKFQPSQAILF
                     KMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(369..611)
                     /gene="yfdY"
                     /locus_tag="ID725_RS24630"
                     /old_locus_tag="pgaptmp_004926"
     CDS             complement(369..611)
                     /gene="yfdY"
                     /locus_tag="ID725_RS24630"
                     /old_locus_tag="pgaptmp_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGV7853334.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            964..1884
                     /gene="lpxP"
                     /locus_tag="ID725_RS24635"
                     /old_locus_tag="pgaptmp_004927"
     CDS             964..1884
                     /gene="lpxP"
                     /locus_tag="ID725_RS24635"
                     /old_locus_tag="pgaptmp_004927"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGV7853335.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2240..2311
                     /gene="ypdK"
                     /locus_tag="ID725_RS24640"
                     /old_locus_tag="pgaptmp_004928"
     CDS             2240..2311
                     /gene="ypdK"
                     /locus_tag="ID725_RS24640"
                     /old_locus_tag="pgaptmp_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGV7853336.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2376..3614)
                     /gene="alaC"
                     /locus_tag="ID725_RS24645"
                     /old_locus_tag="pgaptmp_004929"
     CDS             complement(2376..3614)
                     /gene="alaC"
                     /locus_tag="ID725_RS24645"
                     /old_locus_tag="pgaptmp_004929"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGV7853337.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          968 a          956 c          886 g          968 t
ORIGIN      
        1 ttcaggataa ctttctatga aagtaaactt aatacttttt agcttatttt tattggtctc
       61 tattatggca tgcaatgttt ttgcattttc taattctggt ggtggtagtg agacgagcta
      121 taaagagcca gaaaaaacgt cagcgatgac gacaactcac tctacaaaat ttcagccatc
      181 acaggcgatt ttgtttaaga tgagagaaga tgcaccacca ttaaacctca cagaagaaac
      241 cacgccccct tttccgacaa aggcgaatta tcttattcat cctgtgcgat agcgtctgtc
      301 acctggcagg tgaacaatca tgtctgatgc tgatgtgaac acctgcccaa atgaaccatc
      361 ctgacccact atctcctgcc ataccccacc ataaagcgaa tcatcaagcc acaagccggg
      421 gcaagaaaca taaatatccc cgtcaccagt tcattaccgc cagttaacca catggtgaag
      481 taataaatga gtgcagcgag gatcaccatg ccgaagaaag aagagaccgc cgataccacg
      541 ttcagtacct gaccgatgta tccgcttacg cagacaatac acagggcgag aaacatccat
      601 aaataaaaca tagctcaacc tcctgccgcg ctaaaaaacc tgtaacttca gtcactttaa
      661 tagtaagaca atccttaacc tctggcaatt tttgctaagc gtcaatgaaa acagcatgtt
      721 aaatgcaata ctattgcgtg cggaaaattt tttactttgc acgattaata atcagtagct
      781 gaaagcagtc agccagtgat aaagtacaga taccgcattc atgctcctcg tggttatgtc
      841 ctgactagtc tttacactct ttacaggaac cattgtcgta catgatggcc caaccaattg
      901 agtgtaccgc tgtcgacatg gaatcttctg cgatacaaca attcgtatct acagaaggta
      961 actatgtttc cacaatgcaa attttcccgc gagtttctac atcctcgcta ctggctcaca
     1021 tggtttgggc ttggtgtact ctggctttgg gtacagcttc cttatcctgt tctctgcttt
     1081 ctcggcacgc gtattggcgc aatggcgcga ccattcctga aacggcgtga atctatcgcc
     1141 cgtaaaaacc tggaactttg cttcccgcag cattctgcgg aagaacgcga gaagatgatt
     1201 gccgaaaact ttcgttcact cggcatggct ctggtagaaa ccggcatggc gtggttctgg
     1261 cccgacagtc gcgtacgtaa atggtttgat gttgaagggc tggataacct taaacgcgca
     1321 caaatgcaaa atcgcggcgt aatggttgtc ggcgtccatt ttatgtcgct ggaactgggc
     1381 ggccgcgtga tgggactttg tcagccaatg atggctacct atcgtccaca taataatcag
     1441 ctgatggaat gggtgcagac ccgtgggcgc atgcgctcta acaaagcgat gatcggcaga
     1501 aataatctgc gcggcattgt cggtgcactg aagaaaggtg aagcggtatg gtttgctccc
     1561 gatcaggatt atggtcgtaa aggcagctcc ttcgcgccgt tctttgcggt ggaaaatgtc
     1621 gccacaacca atggcaccta tgttctctcc cgtctctctg gcgcagccat gttgaccgta
     1681 acgatggtaa gaaaagcgga ttacagcgga tatcgtttgt tcatcacccc agagatggaa
     1741 ggctacccga cagatgaaaa tcaagccgct gcctatatga acaagattat cgagaaagag
     1801 atcatgcgcg caccggagca gtacctctgg atccaccgtc gctttaaaac gcgcccggtg
     1861 ggagaatcgt cgttgtacat ttaacaaact gctcttactc tttccctcct caaatcggcc
     1921 ataataacct catggttttt atggcttttt tcttaagatt ttttccttcg ctgaatatct
     1981 aattattacc gccagaaatt aaactgtcgc cgatatgaga cacctgtaag ttactaaaag
     2041 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
     2101 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacaggact tttaaggtcg
     2161 ttttgcccgg tgtcaactca ctgtatcagg tgtgatgaag tcattcaggc gtaaccgtaa
     2221 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
     2281 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
     2341 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
     2401 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc acgaatcgcc
     2461 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtgtc gccgtagtca
     2521 ccaaagccaa tccctggaga gacacagacc ttcgcttcgt taaggagctt cttggcaaat
     2581 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
     2641 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcgcggcgg
     2701 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctccag cgccgcaatc
     2761 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
     2821 gcactgacca gcgttttgtt gccgaccata aagccgatac gccagcccgc catgttgtag
     2881 cttttcgaca gcgtaaagaa ctcgactgcc acatcgcgtg cacccggtac ctgcatgatt
     2941 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
     3001 acatcgtagc gtttcgccag cgccactact ttttcgaaga actccagttc cacgcattgc
     3061 gcggttgggt ttgacgggaa gccgaggatc atcatcttcg gtttcggata actttcaaga
     3121 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
     3181 tgcgccccgg caatcaccgc gccataaata tggatcgggt agcttggatt cggcaccagc
     3241 accgtgtcac catgatccag cgtcgccagc atcaggtgtg ccagcccttc tttcgaacca
     3301 atggtgacga tggcttctga ttccggatcg atttcaacgt cgtagcgatc ctgataccag
     3361 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
     3421 ggacgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
     3481 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
     3541 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
     3601 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
     3661 gccacgatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
     3721 aacattttta ttaatgacgt taaatgagga actctgcttt aaaaacagga aatttatc
//
LOCUS       DABSYT020000215         3765 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.217, whole genome shotgun sequence.
ACCESSION   DABSYT020000215 DABSYT020000000
VERSION     DABSYT020000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.217"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..533
                     /locus_tag="ID725_RS24650"
                     /old_locus_tag="pgaptmp_004930"
     CDS             <1..533
                     /locus_tag="ID725_RS24650"
                     /old_locus_tag="pgaptmp_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGV7853338.1"
                     /translation="FMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKDTPSFGHSCRHS
                     MCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKSYRISRQLGDIW
                     RRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAKQDQKEHGIILR
                     RSRLPEKSNRGRPRHN"
     gene            688..3048
                     /locus_tag="ID725_RS24655"
                     /old_locus_tag="pgaptmp_004931"
     CDS             688..3048
                     /locus_tag="ID725_RS24655"
                     /old_locus_tag="pgaptmp_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGV7853339.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(3210..3386)
                     /locus_tag="ID725_RS24660"
                     /old_locus_tag="pgaptmp_004932"
     CDS             complement(3210..3386)
                     /locus_tag="ID725_RS24660"
                     /old_locus_tag="pgaptmp_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853340.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(3524..3765)
                     /locus_tag="ID725_RS24665"
                     /old_locus_tag="pgaptmp_004933"
                     /pseudo
     CDS             complement(3524..3765)
                     /locus_tag="ID725_RS24665"
                     /old_locus_tag="pgaptmp_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          964 a          789 c          970 g         1042 t
ORIGIN      
        1 acttcatgat tctgaaagac gtgaatgaac acatggaagg tctgtttcaa cgtttttcta
       61 aactgttacc tttcagaatt gattttgcct acaggaaaga cacgcccagt tttggtcatt
      121 cctgtaggca cagtatgtgt attgaaatgc accgcttact tagtgaaacc caaacgatgc
      181 tggcgggata ttactgggta atggaatata cgtcaaacaa gggacttcat attcatttta
      241 tcggctacct ggatgggcaa cgtcacaaca aatcttatcg gatatccagg caactgggag
      301 atatctggag gcggatcacg gaaggggagg gttattttca cctgtgcagg gctaaagaca
      361 aatacccggt gcgtatcgat cacgtaattc actatagtga taaatctgcg gtagacgact
      421 tacgttacgc ccttagctat ctagccaaac aggatcagaa agaacacggt attattctga
      481 ggcgtagcag gcttcccgaa aaaagtaacc ggggccgacc acgacataac tgaattacag
      541 ctgtattagg tattagaatt aagtttctgt aagttgttgg gaggtgcttg agttaatagg
      601 cagaagaggg cggggcgctt tgtcatcagt gtaggggacc cgaatttctg ccgtggtgcc
      661 tcagtggatc ggatacaggt aacgaatatg gttcataaat ctgacagtga tgaattagct
      721 gcattgaggg cagaaaatgc ccgtctggtc tcattacttg aagctcatgg gattgaatgg
      781 cgacgtaaac cgcagactcc tgtgcagcgc gtttctgtat tatccaccga cgagaaggtt
      841 gcattatttc gtcggttgtt tcgtgggcgt gatgatgtat gggcacttag atgggaaagt
      901 aaaaccagcg gcaaatcagg gtactctcct gcctgcgcta acgaatggca ggcgagaata
      961 tgcggtaaac cccggataaa atgtggggac tgcgctcacc gtcagttgat tcctgtatcc
     1021 gatctcgtca tctaccacca tctggccggt acccatactg tcgggatgta tccgttgctg
     1081 gaagatgatt cctgttattt tctggcagtt gattttgatg aagctgaatg gcagaaggat
     1141 gcatccgcat tcatgcgatc ctgtgatgag ctgggtgtac ctgctgcgct ggaaatatcc
     1201 cgttcacgtc agggggcgca tgtctggata ttttttgcct cacgagtttc ggcccgcgaa
     1261 gctcgccgtc tggggactgc tattattagc tatacgtgta gtcggacccg acagctgcga
     1321 ttggggtctt atgaccgatt atttcctaat caggatacta tgccaaaagg gggatttggt
     1381 aatctcatag cgttacctct gcaaaaaaga ccgcgtgaat tggggggaag cgtttttgtt
     1441 gatatgaatc tccagcctta tcctgatcag tgggcttttc ttgtatcggt gaccccgatg
     1501 aatgtgcagg atattgaacc gacgatatta cgggctacag ggagtatcca tcctctggat
     1561 gtgaatttta tcaacgaaga agacctgggt acgccgtggg aagagaaaaa atcatcagga
     1621 aacagactga atatttctat tgcagaaccg ctgaaaatca cgctggcaaa ccagatctat
     1681 ttcgaaaaag cgcaattacc tcaggtgctg attaaccgac ttattcggct ggcagcattt
     1741 ccgaaccctg agttttataa ggctcaggca atgcgtatgt cagtctggaa taagccccgt
     1801 gttataggtt gtgcggagaa ttacccgcaa cacattgcgt tgccccgggg atgtctggac
     1861 agcgtattat ctttccttag ggacaacaat attgctgcag aattaatcga taaacgattt
     1921 gccgggacgg aatgtaatgc cgtttttatg ggaaacctca gagcggagca ggaagaggcc
     1981 gtttcggcat tactccgtta tgacactggt gtgctttgtg cgccaacggc ttttggtaag
     2041 acagttaccg cagcggcagt gattgccaag cggaaagtga atacactgat actggtacac
     2101 cggactgaat tgctgaagca gtggcaggag cgtctcgcgg tgtttctgca ggccggtgac
     2161 agtattggta ttatcggggg aggtaaacat aaaccctgtg gcaatattga tattgcggtg
     2221 gtgcagtcca tatccagaca aggagaagtt gaacctctgg tcaggaatta tgggcaaatc
     2281 attgtggatg agtgccatca tattggcgcg gtttcatttt ctgcgattct gaaggaaacg
     2341 aatgccagat atctgcttgg cctgacggca acaccaatcc gacgggatgg tctgcatccc
     2401 attattttta tgtactgtgg tgccattcgc catacagcgg tccgcccgaa ggaaagccca
     2461 cataatctgg aggtactgat ccgttcccgt tttacatctg gtcatttacc atcggatgcg
     2521 agaatccagg atattttcag agaaattgct ctggatcatg acagaacggt ggcgatagct
     2581 gaagaagcca tgaaagcttt cgggcagggg cgaaaagttc tggtactgac tgaacgtaca
     2641 gatcatctgg atgagatagc atcagtgatg aattcactga aattgtctcc ctttattctc
     2701 catggtcgac tatcgaaaaa aaagcgtgcg atgctgatat ccgggctgaa tgctcttcct
     2761 cccgattctc ctcgaatttt gttgtcaaca ggcagactta ttggtgaggg atttgaccac
     2821 cctccgctgg atacgctgat tcttgccatg cctgtgtcat ggaaagggac attacagcag
     2881 tatgcagggc gtcttcacag agagcatacc ggtaaaagcg atgtcaggat cattgatttt
     2941 gtggataccg cgtatcctgt gctgctcaga atgtgggata aacgtcagcg gggttataaa
     3001 gcgatggggt acaggattat agctgacggc gatgaatcag tgatttaatt ttaaggagta
     3061 ctttaccctt tttaaataat ttgagctccc cgggttgttc gggcaacaaa gagtcccagt
     3121 atacaggctt caggaatatg ttgtagtatc aggcttttca tcgctttaaa atgactaccg
     3181 gcagtgagaa cgtcatcaaa aataacaatc tagggcctgc acccttccag taattgttca
     3241 tccagttggt agtttgccat aatcgcttct ggtaagggcc ggtttgttgc ggtatgagag
     3301 gctattgttg atacgttttg cacaaccagt tcccagaaat ccagccatgg ttcgtaggtt
     3361 tgcgcaattc gcagaatttc tgtcatacgg tcatcgtaag cggcatctgt acggcatttg
     3421 gatggtggta cgggaacaaa tgtgtattcc tcaagattac cgatatttga tatgagcatc
     3481 tgagcaacat actcaatagc agccttttta tagtggtaat caggctacga aagtacagcg
     3541 ttttataaat tgtctcaggt gatattcgca gcgttttttg acgtggtttt gttcgcctta
     3601 accatcctga tatttgctct ggagaccatt tcatctccag cttttccaga acaagctttc
     3661 gcaatggtaa attttgatcc agtaagcacg gttttggcct tttcgccatt ctgttggctc
     3721 ggttattagc atcaacagct ttgtaatagc gtctgccccg attac
//
LOCUS       DABSYT020000216         3700 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.218, whole genome shotgun sequence.
ACCESSION   DABSYT020000216 DABSYT020000000
VERSION     DABSYT020000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.218"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(66..482)
                     /locus_tag="ID725_RS24670"
                     /old_locus_tag="pgaptmp_004934"
                     /pseudo
     CDS             complement(66..482)
                     /locus_tag="ID725_RS24670"
                     /old_locus_tag="pgaptmp_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(482..667)
                     /locus_tag="ID725_RS24675"
                     /old_locus_tag="pgaptmp_004935"
     CDS             complement(482..667)
                     /locus_tag="ID725_RS24675"
                     /old_locus_tag="pgaptmp_004935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853341.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            844..3054
                     /gene="katP"
                     /locus_tag="ID725_RS24680"
                     /old_locus_tag="pgaptmp_004936"
     CDS             844..3054
                     /gene="katP"
                     /locus_tag="ID725_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HGV7853342.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            3098..3487
                     /locus_tag="ID725_RS24685"
                     /old_locus_tag="pgaptmp_004937"
     CDS             3098..3487
                     /locus_tag="ID725_RS24685"
                     /old_locus_tag="pgaptmp_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGV7853343.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            3544..3700
                     /locus_tag="ID725_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /pseudo
     CDS             3544..3700
                     /locus_tag="ID725_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3600..>3700
                     /locus_tag="ID725_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1045 a          781 c          952 g          922 t
ORIGIN      
        1 aggagcgaac gtctggttca gcagatttgg cgcaactggc agattgtggt tcgagttcgt
       61 agtcgcacat ctgcctggcg gcagatttcc tgtatcacat ccgcagcaat gcggctcatc
      121 tctgccagta accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac
      181 acaatatgct gccacggaca gtcgggaacc agactcagca gatactgtat ccactgtgcg
      241 ccagccttca ctccgcagtg cgggcaggag cgacttttac accggaagca gacctttttt
      301 atgtggctgc agtccggaga tgaacagcac cactgtgtgt accccatcag cgtggtcccg
      361 caggccatga tttttgtcac agactcaatg actaccggac gcactgaacc ttccggttgt
      421 ttctccagcc agttaagcca gcggtttccc tgctgaaaaa tgtcggcaaa acggggaagc
      481 atcagaaggg cgggggaact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca
      541 tgccgccggc gctgataccg gcaagaatgg tcgcaaactc ccgctccgtg cagcgggcta
      601 tttcaggata cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttctga
      661 catccacaaa gaagggaata ttcaggtctg cgcagcactc aacggcatcg tcagttgcgg
      721 cttggaaccc cttagtattt tttgtctgta gtatctatcc cagcaatagg tatatcctgt
      781 tgcatcaata aagttgactt ttgtatacaa catgcgaatt tcccttaatc cggagctatt
      841 cgtatgataa aaaaaactct tcctgttctg attcttctgg cgctatcggg gagcttttct
      901 accgctgtag ccgctgataa aaaagagact caaaatttct actatccaga aacactggat
      961 ttaactcctc tgagattaca cagccctgaa tcaaatccct ggggggctga ttttgattat
     1021 gccaccagat ttcaacagct ggatatggag gctctgaaaa aagatatcaa agatttgctg
     1081 acaacttccc aggattggtg gcctgcggat tatggtcatt atggtccttt ctttattcgt
     1141 atggcttggc acggtgccgg aacatacagg acatatgatg gccggggagg cgccagtggt
     1201 ggtcagcaac gttttgaacc gctgaacagc tggccggata acgttaatct ggataaagcc
     1261 cgtcgattgc tgtggccagt caagaaaaaa tacggctcca gtatttcctg gggagacctg
     1321 atggtcctga ctggtaatgt tgcccttgaa tccatgggat ttaaaacgct gggatttgct
     1381 ggcggaagag aagatgactg ggagtcggac ctggtatact gggggcctga caacaagcct
     1441 cttgcagata accgggataa aaacgggaaa cttcagaaac ctcttgccgc cacgcagatg
     1501 ggacttattt atgtcaatcc tgaaggcccc ggtggaaaac cagatcctct ggcttccgcg
     1561 aaagatatca gggaagcttt ttcacgtatg gccatggatg atgaggagac tgtggccctg
     1621 atcgcgggag ggcatacatt tggtaaagca catggtgcag cgtctcctga aaaatgtatt
     1681 ggcgcagggc ctgatggtgc acctgtggag gagcagggac tgggatggaa aaataaatgt
     1741 ggtacaggaa acggcaaata taccatcacc agtggcctgg aaggagcctg gtcgacatcg
     1801 ccaacccagt tcacaatgca gtatctgaag aatttatata aatatgaatg ggagctgcac
     1861 aagagtcctg ccggtgctta tcagtggaag cctaaaaaag cggcaaatat agttcaggac
     1921 gcgcatgatc cgtctgtcct gcatccgttg atgatgttta cgacggatat tgctcttaaa
     1981 gttgatcctg aatataagaa aataaccacc cgtttcctga atgatccaaa agcttttgag
     2041 caggcattcg caagagcatg gtttaaactg acccaccggg atatgggacc ggcagcccga
     2101 tatcttggta atgaagttcc tgcagaatca tttatctggc aggatcctct tcctgcggcg
     2161 gattatacaa tgattgatgg taaagacatt aagtcgctga aagagcaggt tatggatttg
     2221 ggtatccctg catctgagct gataaagaca gcctgggctt cagcttccac atttcgtgtg
     2281 actgattatc gtgggggaaa taatggtgcc cgcatcaggt tacagcccga aattaactgg
     2341 gaagttaatg agcctgaaaa actgaagaaa gtactggcat ccctgacctc attacagcgt
     2401 gaatttaaca aaaaacagtc tgacggaaag aaagtgtcgt tggctgattt aattgttctt
     2461 tcgggtaatg ctgcaatcga agatgcggcc agaaaagccg gggtggaact tgagattccc
     2521 tttactccgg gaagaactga cgcctctcag gagcagacgg atgttgcctc attcagtgta
     2581 ctggagccga cagcagatgg attcagaaat tattactcaa aaagcagaag tcatatatcg
     2641 ccggttgaaa gcctcattga taaagccagt cagctggatc tcaccgttcc tgaaatgacg
     2701 gcattactgg gtggtctgcg ggtaatggat attaatacaa ataattcttc gttgggagtg
     2761 tttaccgata cccctggtgt tctggataac aagttttttg ttaatctgct ggatatgtca
     2821 acacgatgga gtaaagcaga taaagaagat acatacaatg gattcgatcg taaaacggga
     2881 gcattaaaat ggaaagcatc ctctgttgat ttaatcttca gttcaaatcc tgaattacgt
     2941 gcggtggcag aagtatatgc ctcggatgat gcgagaaata agtttattca tgattttgtt
     3001 aaatcgtgga ataaagttat gaatagcgat cggtttgatt taaacaataa atgataaaac
     3061 tgatgaggga gggcacctcc ctcatgaagg agaaattatg cagaaaagca ataaagttat
     3121 aatcagctgt ggtatggcat tatatttttt gtgtgggggg attacagtgg cagaagaact
     3181 tgatgaaagc atgcaagttc ttgcaaggaa tatgagtgta cttcagtcat caatggataa
     3241 aaaggagctt cttgaagctc ttgacagaat ggatgatgct gtagatgagt caatgaagcg
     3301 tcttcctgag agcatttcac cggctgatga agaagggaag aatgactata taaaagaact
     3361 tcaaaagctt gaaaaagaga ttatgctggc gaaacaaaaa gtcttatcgg gacagttatc
     3421 agatataccc gattcggtaa acaagatgaa taaaatcaga gttgaggggc atgataaatt
     3481 cagataaatc agttggttga ttcctgcggg acgaatacat acgtcaggaa cataacttta
     3541 tggctggaac gtgaaaatcg tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc
     3601 gcttattttg cgaaggcgga gttcgaccgc ctctggaaaa aataatgcca ctgctggata
     3661 agctgcgtga gcagtacggg gtcggaccgg tatgcagtga
//
LOCUS       DABSYT020000217         3600 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.219, whole genome shotgun sequence.
ACCESSION   DABSYT020000217 DABSYT020000000
VERSION     DABSYT020000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.219"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..213)
                     /locus_tag="ID725_RS24695"
                     /old_locus_tag="pgaptmp_004939"
     CDS             complement(<1..213)
                     /locus_tag="ID725_RS24695"
                     /old_locus_tag="pgaptmp_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853344.1"
                     /translation="MKKTLTQQGAFRKERKALQRAIANGLTEKDIVMEMVKRMDNPDS
                     ATTLNQASAAVMYLTALCNKETPITDA"
     gene            464..1267
                     /locus_tag="ID725_RS24700"
                     /old_locus_tag="pgaptmp_004940"
     CDS             464..1267
                     /locus_tag="ID725_RS24700"
                     /old_locus_tag="pgaptmp_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HGV7853345.1"
                     /translation="MGIKNSKKPAIQAYSELQQAYDFFNATLFEQMLPDMLITLQRGK
                     NTFGYFAPDRFTGESDLSELAMNPDYFGGRSLVDTLSTLVHEMVHVWQHYTPGIKKCR
                     GGYHDRVWGEKMEQIGLMPSNTGLPGGRKTGQQMTHYIIRGGRFQTAVYDLLKSGFSI
                     SWYDKWSEGIIAVSKINTDVLDDWLRVTDKSDRELINKLTQTVSKRNTLPDNNESVSG
                     LEPDLIIDIKPPTKGTGTRSKFTCPGCGLNAWAKPSAVLVCGECRVELE"
     gene            1303..1518
                     /locus_tag="ID725_RS24705"
                     /old_locus_tag="pgaptmp_004941"
     CDS             1303..1518
                     /locus_tag="ID725_RS24705"
                     /old_locus_tag="pgaptmp_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853346.1"
                     /translation="MEQCIFDLGIINYNPVITWTVPVAFFFLVLLAGIVFNIGRKSNL
                     IVFVLAMLLPVTTVFIFMLLGGKINMC"
     gene            1582..1863
                     /locus_tag="ID725_RS24710"
                     /old_locus_tag="pgaptmp_004942"
     CDS             1582..1863
                     /locus_tag="ID725_RS24710"
                     /old_locus_tag="pgaptmp_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853347.1"
                     /translation="MNYDEITKITAELIDSYMTQAVNADSTGVAEMYHNAAWGVRTLW
                     FDLASKIELDTIKKNKYTNYELRRKFDEQNEEFKQRTDRNRVPLLKSPE"
     gene            complement(2171..2650)
                     /locus_tag="ID725_RS24715"
                     /old_locus_tag="pgaptmp_004943"
     CDS             complement(2171..2650)
                     /locus_tag="ID725_RS24715"
                     /old_locus_tag="pgaptmp_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HGV7853348.1"
                     /translation="MATEWVDVADNAVKIGLGSLLTILGGWLTLKLTQRHELKKEAAV
                     QRLKDKEIKTERYVEFLALSQSLMQKYLYEQCEANNDDYLAYLRIHNEITITSSLAIR
                     KAAFKLQLDVSTFIFYNKIHDIELINALRDEARNSVSIFQAIVNEEICNGKFGTASQ"
     gene            2768..3214
                     /locus_tag="ID725_RS24720"
                     /old_locus_tag="pgaptmp_004944"
     CDS             2768..3214
                     /locus_tag="ID725_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853349.1"
                     /translation="MIQSLGTIMSSSAAVTVLTGVTVFVVGQLIAKRFIEPYISFREQ
                     LGRITALLLREQATITNFRANHETIHDLKDAASQLMAKYAALPGSLKRSYLGIKFVPS
                     KGEVLGAAQNLNEITSILAGNSKENTYNLIKEIGLKLNIPTTYSSH"
     gene            3291..3434
                     /locus_tag="ID725_RS24725"
                     /old_locus_tag="pgaptmp_004945"
     CDS             3291..3434
                     /locus_tag="ID725_RS24725"
                     /old_locus_tag="pgaptmp_004945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853350.1"
                     /translation="MAMTGENCSTPNAYILFTAFDIFLAFNAFNAFNAFKGGKIEQLT
                     DDE"
BASE COUNT         1016 a          709 c          760 g         1115 t
ORIGIN      
        1 agcatcagtg attggggttt ctttattgca aagcgcagtc agatacatga cggctgctga
       61 agcctgattc aaagttgttg ctgaatctgg attatccatt cttttgacca tttccatgac
      121 aatatctttt tcggttaaac cgttagctat tgcgcgttgt aatgccttac gttctttacg
      181 gaatgcgcct tgttgggtaa gtgttttttt cataaaacct cttgacttat gagctttatt
      241 gtgagggtgt gcttaataag ctatcagagg tttttatgcc gttcaatacc ttgctgtcat
      301 cattagcagc tcaatgtcaa tgtaaatggt acaactatat gtattattgt gattttgttt
      361 atgagcattt agtgcgctgc tgtgtattga tgcttctttc gccgcaataa attgtattgt
      421 acaatacaat cccgtgcatt gatggctaaa caggggcgag tgtatgggaa taaaaaacag
      481 taaaaaacca gcaattcagg catactctga attgcagcaa gcctatgatt tttttaatgc
      541 aacgctgttt gagcagatgt tgccggatat gttgattacg cttcagcgtg gtaaaaacac
      601 atttggttat tttgcgccgg acaggtttac tggtgagtct gatttgtctg aactagcaat
      661 gaaccctgac tacttcggag ggcgttcatt ggtcgatacg ctttctacgc ttgtgcatga
      721 aatggtgcat gtctggcagc actatacacc cggtataaaa aaatgtcgtg gtggttatca
      781 tgatcgggta tggggagaaa aaatggaaca aattggcctg atgccttcaa atactggatt
      841 accaggaggt agaaaaacgg gacagcaaat gacgcactat attattcgtg gtggtcgttt
      901 tcagacagcc gtttatgatc tcctgaaaag tggtttctcc atttcttggt atgataaatg
      961 gtctgaggga attattgctg tatctaaaat taacactgat gttcttgatg actggttgcg
     1021 tgttactgat aaaagtgatc gggaacttat taataagctg acacaaacgg ttagtaagcg
     1081 gaacacatta cctgataata atgagtccgt ttcaggtctg gaaccggatc tcataattga
     1141 tataaagcct ccaacaaaag gaacgggaac tcgttcaaag tttacgtgtc ctggatgtgg
     1201 tttaaatgca tgggcgaaac cttcggctgt ccttgtgtgt ggtgaatgca gggttgaact
     1261 tgaataagtt aataaaatta tttacaggag aaatttaaat ctatggagca atgcatcttt
     1321 gatttaggta ttataaacta taatcctgtc attacatgga ctgtacctgt tgcatttttc
     1381 tttttagtat tgctggctgg tatagtattc aatattggca ggaagagtaa tctgattgtt
     1441 tttgtcctgg caatgttgct cccagtaacc acagttttca tttttatgtt gctgggcggt
     1501 aagataaata tgtgttgaat cgctatggtc gtcttgtttt gcgacctttc attaattcca
     1561 atagcctcgc agggatataa aatgaattac gatgaaataa caaaaatcac cgctgaactt
     1621 atagatagtt atatgacaca agctgtcaac gcagactcta caggagttgc tgaaatgtat
     1681 cataatgcag cgtggggagt acggactttg tggttcgacc ttgcttcaaa aatagagtta
     1741 gatacaatta aaaaaaataa atatacaaat tatgagttaa gaagaaaatt tgacgaacag
     1801 aatgaagaat ttaagcaacg aacagatcgt aaccgagtcc ctttactgaa atcgccggaa
     1861 taatgccaac agtaacattg cgagcattcc aacacttgcc gctacacggt tagctgcaag
     1921 tgtgccgagc cgccctcgat gcggtagcag aaccgcttcc ttccttttta cgcatcgtga
     1981 catttttcaa aaaaactcac caatattctc ctcggcagaa atgacataac tcactgtaaa
     2041 ataacaaatc atctaaacgc cggctttgta tcgagcgcgt tggccaacgc ctttatcagc
     2101 tgcgaccgca ccagataatt tgaataagat ttcctgcatc acctggacat acaggttatt
     2161 ctgtcctgcg ctactggctt gctgtgccaa actttccgtt gcagatttcc tcgttcacaa
     2221 tggcctgaaa tatggagaca gaatttctag cctcatccct cagtgcatta atgagttcta
     2281 tgtcgtgtat tttgttgtag aaaatgaaag tggaaacatc aagctggagt ttaaatgctg
     2341 ctttccttat ggctaaactt gacgtgatcg ttatctcatt atgtatgcga agataggcaa
     2401 gataatcatc gttatttgct tcacactgct catataaata tttttgcatg agagattgag
     2461 acaaggcaag aaattccaca tatcgttcag tttttatttc tttatctttt aatctctgta
     2521 ctgccgcttc tttttttaat tcgtgcctct gtgttagctt aagagtcagc caacccccta
     2581 aaatagtcaa caacgacccc aaacctattt taacggcatt atcagccaca tccacccact
     2641 ctgttgccat ctgttacgcg ttcttcctgt tatgctcatg gctgtcagta taaatccccg
     2701 gctgactgga catacagcat tttctgtaca gtagacagac agttgccctc cagataggat
     2761 aacccccatg attcagtcac taggtacaat tatgagttcg tctgctgccg taactgttct
     2821 gaccggcgta accgtttttg ttgttggtca actcatcgca aaaagattca ttgagcctta
     2881 tatatcattc agagaacaat tagggaggat tacagcgctt ttattacgtg agcaagcgac
     2941 gatcacaaat ttcagagcta atcatgaaac catccacgat ttgaaagatg ctgcttctca
     3001 gttaatggca aagtatgcag ccttgcctgg tagcctaaaa agaagctatt taggtataaa
     3061 gtttgtgcca tcaaaggggg aagttcttgg tgcagcacaa aaccttaatg agatcacctc
     3121 aatacttgct ggcaacagta aggaaaatac ctacaatctc ataaaggaga ttggactcaa
     3181 gctcaatata ccaactacct attcttctca ttaatccccg cgttacctag acattcaggt
     3241 aaaaaagtcc aactggtgag gttgggcttt ttggctttcc ggttctgttg gtggctatga
     3301 ccggagaaaa ttgctctaca cctaacgctt acattttatt tacagcgttt gatatcttcc
     3361 tcgcctttaa cgcctttaac gcctttaacg cctttaaggg ggggaagata gagcaactca
     3421 ccgatgatga ataaagtgga cgaagagacg ataactacac tcccatccag gggggctttt
     3481 tttgtggtac tgtagtgtgg gagtaagttc gggtctcata ttgaatgagg cgattgcgag
     3541 ctgcctttca tcgaaaacta atgtccatgt cattgtattg tctgactcta tctcgtaaca
//
LOCUS       DABSYT020000218         3471 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.220, whole genome shotgun sequence.
ACCESSION   DABSYT020000218 DABSYT020000000
VERSION     DABSYT020000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.220"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            232..1164
                     /gene="rfaD"
                     /locus_tag="ID725_RS24730"
                     /old_locus_tag="pgaptmp_004946"
     CDS             232..1164
                     /gene="rfaD"
                     /locus_tag="ID725_RS24730"
                     /old_locus_tag="pgaptmp_004946"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGV7853351.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1174..2220
                     /gene="rfaF"
                     /locus_tag="ID725_RS24735"
                     /old_locus_tag="pgaptmp_004947"
     CDS             1174..2220
                     /gene="rfaF"
                     /locus_tag="ID725_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGV7853352.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2224..3216
                     /gene="rfaC"
                     /locus_tag="ID725_RS24740"
                     /old_locus_tag="pgaptmp_004948"
     CDS             2224..3216
                     /gene="rfaC"
                     /locus_tag="ID725_RS24740"
                     /old_locus_tag="pgaptmp_004948"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGV7853353.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3213..>3471
                     /locus_tag="ID725_RS24745"
                     /old_locus_tag="pgaptmp_004949"
     CDS             3213..>3471
                     /locus_tag="ID725_RS24745"
                     /old_locus_tag="pgaptmp_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGV7853354.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQ"
BASE COUNT          883 a          842 c          887 g          859 t
ORIGIN      
        1 cattcaaatg cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac
       61 atgcaaaacc aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt
      121 agcatccttg cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca
      181 gacattcgtg tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc
      241 gttaccggcg gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc
      301 atcaccgata ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg
      361 gatctgaata tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc
      421 gaagagttcg gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag
      481 tgggacggca agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac
      541 tgcctggagc gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc
      601 acctccgact ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca
      661 aaattcctgt ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt
      721 ggcttccgct atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc
      781 gtcgctttcc atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt
      841 agcgagaact ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg
      901 ttcctggaaa atggcgtttc cggcatcttc aacctcggca ccggtcgtgc ggaatccttc
      961 caggctgtag ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg
     1021 ttcccggata agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg
     1081 cgcgcggcgg gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg
     1141 gcctggctga atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct
     1201 tgggttggcg acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc
     1261 caggcgataa tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg
     1321 gaagttaacg aagctattcc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc
     1381 cgcaaactgg gtcatagtct gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac
     1441 tccttcaaat ccgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc
     1501 ggcgagatgc gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg
     1561 ctaatggtgg aacgctatgt cgcgctggcc tatgacaaag gcattatgcg cacagcacaa
     1621 gatctgccgc agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat
     1681 acctgtaatc aattttcgct ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg
     1741 gagtttggtc cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg
     1801 attgatgaag gttatcaggt ggttctgttt ggctctgcga aagatcatga agcaggcaat
     1861 gagattcttg ccgctttgaa taccgagcag caggcatggt gtcggaacct ggcgggggaa
     1921 acacagcttg atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat
     1981 tctggcctga tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtccg
     2041 agtagcccgg acttcacacc gccgctatcc cataaagcgc gcgtgatccg cctgattacc
     2101 ggctatcaca aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac
     2161 attactcccc agcgcgtact gaaagaactc aacgcgctat tgttacaaga ggaagcctga
     2221 cggatgcggg ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc
     2281 gcactcactg atgcccagca ggcaatccca gaaattaagt ttgactgggt ggtggaagaa
     2341 gggttcgcac agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata
     2401 cgtcgctggc gtaaagcctg gttctcggcc cctataaaag ctgaacgcaa agcgtttcgt
     2461 gaagcgctac aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc
     2521 gcggcgctgg ttacgcgtct ggcacatggc gtaaagcatg gcatggactg gcaaaccgct
     2581 cgcgaacctt tagccagcct gttttacaat cgtaagcatc atattgcaaa acagcagcac
     2641 gccgtagaac gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc
     2701 cagggcgatt atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa
     2761 tatgccgtat ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg
     2821 cgagaattga ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg
     2881 ccgcatgagg aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg
     2941 ccgaagatga gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg
     3001 gtggatacgg ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat
     3061 ggaccaaccg atccgggatt aattggtggg tatggggaga atcaagtaga gtgtcgttct
     3121 acaagtatgt ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa
     3181 ataataacca ataagttgac atcggagata agatgacctc aacattattt ttctctctcg
     3241 agaaaaaaaa ctggatagcg tactggaaca gagctctcgt attcttattc attaccacct
     3301 attttttggg tgggattaca aggtataaac atcttattgt tattcttatg acaataacga
     3361 caatcgtcta tctctgcaaa cggccaaaac actatctctc actatttaaa acatttcttt
     3421 ttggtagtgt tgccatatta actattgctg cattgctgtc acttcttcaa t
//
LOCUS       DABSYT020000219         3455 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.221, whole genome shotgun sequence.
ACCESSION   DABSYT020000219 DABSYT020000000
VERSION     DABSYT020000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.221"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            31..390
                     /locus_tag="ID725_RS24750"
                     /old_locus_tag="pgaptmp_004950"
     CDS             31..390
                     /locus_tag="ID725_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGV7853355.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            399..929
                     /locus_tag="ID725_RS24755"
                     /old_locus_tag="pgaptmp_004951"
     CDS             399..929
                     /locus_tag="ID725_RS24755"
                     /old_locus_tag="pgaptmp_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGV7853356.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1171..1368
                     /locus_tag="ID725_RS24760"
                     /old_locus_tag="pgaptmp_004952"
     CDS             1171..1368
                     /locus_tag="ID725_RS24760"
                     /old_locus_tag="pgaptmp_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGV7853357.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1519..2577
                     /locus_tag="ID725_RS24765"
                     /old_locus_tag="pgaptmp_004953"
     CDS             1519..2577
                     /locus_tag="ID725_RS24765"
                     /old_locus_tag="pgaptmp_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGV7853358.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            2618..2693
                     /locus_tag="ID725_RS24770"
                     /old_locus_tag="pgaptmp_004954"
     tRNA            2618..2693
                     /locus_tag="ID725_RS24770"
                     /old_locus_tag="pgaptmp_004954"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2651..2653,aa:Ile,seq:cat)
     gene            2774..2850
                     /locus_tag="ID725_RS24775"
                     /old_locus_tag="pgaptmp_004955"
     tRNA            2774..2850
                     /locus_tag="ID725_RS24775"
                     /old_locus_tag="pgaptmp_004955"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2808..2810,aa:Arg,seq:tct)
     gene            3374..>3455
                     /locus_tag="ID725_RS24780"
                     /old_locus_tag="pgaptmp_004956"
     CDS             3374..>3455
                     /locus_tag="ID725_RS24780"
                     /old_locus_tag="pgaptmp_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGV7853359.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKE"
BASE COUNT          891 a          748 c          982 g          834 t
ORIGIN      
        1 actggcgtgc tcgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
       61 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttgtatc aaaagccggg
      121 gagcgttatc gccgggcagt ggtgcttatt gttcgccagc agcgactgaa attaagcctg
      181 tccggacggc tggcaataaa aattattgca gagccaccgg ataagcgtcg tcgtgacctg
      241 gacaatattc tgaaagcacc gctggatgcg ctgacgcatg cgggagtgtt aatggacgat
      301 gagcagtttg atgaaatcaa tattgtacgt ggtcagccag tatctggtgg acggctgggt
      361 gtgaagattt acaaaattga gagtgagtga gcgtaaatat gatatatccg gaaattacag
      421 gcaaaagcgg cgaacattta cgcctgaaca cgctggaagc agtctggatc caggggaaat
      481 tacggatgtg ggggcggtgg tcgtatatcg gtgggggtaa atccggaaat atgtttaacc
      541 ggttactggt ttcgaaaaag ctgacgaaaa cagcagttaa tgaggtttta cgcagcatga
      601 agaaatccgg gctggaaaaa ccggaacttg aggcattttt tcgggatatg accagaggga
      661 agcagaagag ctggttgtca cattgtacag acacagaggc gttgattatt gatcgcgtta
      721 tcagtgaggt gcttggggaa tatcccgggc taatcaatat tctccggcaa aggtacgaag
      781 gacggggaat gagtaagaga aaaatggcag aatgtttaaa tcgtactcac ccggaatggt
      841 gtttcagcac atgtgagaaa cgtattgcag gttggttagc cgtggctgaa cacatgcttt
      901 atgtacctat gcacgattca tttcgataaa aaaagcttgc ttttttacgc agaaacagct
      961 tgaattcctg taagcttcgc aaagctgtat cgcgaggcga aatgcaagtt ttttcgcaca
     1021 aggaagccac cggaaggtgg tttttttgtg tccgtaatat acagcagcgc aataaattcg
     1081 ctggtggtta ttaataccgt tctttcaggt tgctggcttt ttcgacaaga gttattggtg
     1141 tgtcacgtta accggaaaag ggaaaaagac atgctgaaac agcaggatat gacagaaacc
     1201 gccagagtgg tgtttaatga attaagcgtt accgacccgg cgacagtcgg ggagattgcg
     1261 cagaatactt acctttcacg cgaacgctgc cagttaatac tgacccagct ggttatggcg
     1321 ggtctggcag actatcagtt tggttgttac agacgccttc agtcctgaag gcttttttat
     1381 ttgtggtaaa tgggcggctg gtgggtgtaa ggggcaccca ccagccatct gctcatgcgt
     1441 tgggttcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg agcctatcag
     1501 agacaggctt aatgatccat gcttaatact gtaaaaatat ccagttgtga gttaatcaac
     1561 gccgactgcc tggaatttat gcggtcgtta cccgaaaatt ctgttgacct gatagtcacg
     1621 gacccgccgt acttcaaagt gaaacccgag ggctgggata accagtgggc gggtgatgaa
     1681 gattacctga agtggctgga ccagtgtctt gcgcagttct ggcgggtgct gaaacctgcc
     1741 ggaagtcttt acctgttctg tggccatcgt ctggcatctg acaccgaaat catgatgcgt
     1801 gagcggttta acgtgctgaa ccatatcatc tgggcaaagc cgtccggacg ctggaacggg
     1861 tgcaacaagg aaagcctgcg ggcgtatttc cccgccacag agcgcattct gttcgcagag
     1921 cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg taggacactg
     1981 aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc tgtcctgggg
     2041 ataacggcaa aacagattgc agatgccaca ggaaagaaaa acatggtgtc gcactggttc
     2101 agtgccggtc agtggcagct gccgaacgaa agcgattatc tgaaattaca ggcactgttt
     2161 gcccgggtgg cagaagagaa gcatcagcgg ggtgaactgg aaaagcccca ccaccagctg
     2221 gtggatacgt atgcctctct gaaccgacag tatgcggagc tgcagagtga atataagcat
     2281 ctgcggcggt atttcggtgt gacggtgcag gtgccgtaca ccgatgtgtg gacgtataaa
     2341 ccggtgcagt actatccagg gaaacatccg tgcgaaaaac cggcagaaat gttgcagcag
     2401 ataatcagcg caagcagtcg tccgggagac ctggttgcag atttcttcat ggggtcgggg
     2461 tcgacagtga aagcagcgat ggcgctggga cgtcgtgcaa ctggcgttga actggagact
     2521 gaacgttttg agcagacggt gcgggaagta caggatttaa tcattcgtaa cggatgagat
     2581 tgcggagtta atcatgcgtc gttattattc agcaatcggc cctttagctc agcggtgaga
     2641 gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccaaccgcca
     2701 ctagctcatc aggaaagaac gtcaccctgt gcgagattcg gagtccccgg tggcggtcca
     2761 ttatcggtat tctgcgttgt tagctcagcc ggacagagca attgccttct aagcaatcgg
     2821 tcactggttc gaatccagta caacgcacca caccacactt atctgccctg actctctttt
     2881 gcgggctttt tattacagga aagacaccgg acagtgaaat gttaaatgcc tcacaattca
     2941 ggcagttgac tgttgcctga catgctgagc gtttgttaaa aaaatcctgc atgatgaatc
     3001 cccctgagcg gcggggcata atgacagatg tttggttgcg tattgtatag gcaagttgcg
     3061 gattctgtct ggtcattgca gaattcaccg ggaggcaccc ggcatcatgc tgtatacaga
     3121 gattaggcat atatccaggc ttctcatcgc aggagccttt ttacatgcaa aaaaaagccc
     3181 gagtgggttc gggcaacagc atgagatact tgcattgtca tttttatcgt gtggatttta
     3241 accagggttt ataaggctgc gcaactgcgc ggcctttttc gttttgcggg ctgcggttct
     3301 cctcttttga ttctccttgt ggccggaccg tggcccgcaa ctgttgagga aaatcccgga
     3361 aaggggagga ataatgacat ttaaacatta tgatgttgtc agggcggcgt cgccgtcaga
     3421 ccttgcggaa aagctgacac acaaactgaa agagg
//
LOCUS       DABSYT020000220         3427 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.222, whole genome shotgun sequence.
ACCESSION   DABSYT020000220 DABSYT020000000
VERSION     DABSYT020000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.222"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(102..410)
                     /locus_tag="ID725_RS24785"
                     /old_locus_tag="pgaptmp_004957"
     CDS             complement(102..410)
                     /locus_tag="ID725_RS24785"
                     /old_locus_tag="pgaptmp_004957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853360.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGAGYF
                     "
     gene            642..1698
                     /locus_tag="ID725_RS24790"
                     /old_locus_tag="pgaptmp_004958"
                     /pseudo
     CDS             642..1698
                     /locus_tag="ID725_RS24790"
                     /old_locus_tag="pgaptmp_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1917..2507)
                     /gene="espM1"
                     /locus_tag="ID725_RS24795"
                     /old_locus_tag="pgaptmp_004959"
     CDS             complement(1917..2507)
                     /gene="espM1"
                     /locus_tag="ID725_RS24795"
                     /old_locus_tag="pgaptmp_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HGV7853361.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            2691..3338
                     /gene="nleG"
                     /locus_tag="ID725_RS24800"
                     /old_locus_tag="pgaptmp_004960"
     CDS             2691..3338
                     /gene="nleG"
                     /locus_tag="ID725_RS24800"
                     /old_locus_tag="pgaptmp_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HGV7853362.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
BASE COUNT         1043 a          767 c          634 g          983 t
ORIGIN      
        1 cttcatctat gtaagacatt tcttcaatat taactaaaaa tgaacatgaa tgtaatcgcc
       61 gctgtaacaa ttcaattctg ttatcattac tatgcataaa ttcagaaata acctgccccc
      121 atatatgtac tgaatacatc aagaaatgaa ttgcctccat tcaattgatt ttcaacagaa
      181 tcagccaatc taatgatatc ggctagttct gtatggctta aacccgaata tagttcccct
      241 tgaacaggat ctacaataaa agcgtccata gcactctgat agcgaacaga aaacgatctt
      301 tctcctattg tgagtctttc catttgatga tctctggtcg cacgttgtaa aacttcaaca
      361 tcttgtgttg acaagtaatt tccagaagac aaattaaatg aaaatggcat aattttatcc
      421 ctaaagcata tatcttactt ttcgataatt aacaaaaagc catcctccac agcaatactc
      481 catcagaata attgtaggat tattttaatc taatttataa gcataaaata tatactcact
      541 aaatagctgc gcagaatagt agatcattta gagggaactc aacccgattg tgcgatctga
      601 tcaatcgcca aatcaataca aatcaccaac cggactgagc aatgtcgatc ataccaccaa
      661 tttcccgtga cgaacgacgc ctaatacaga aagccatcca taaaaggctg acagccatgc
      721 tgatgctgca ccggggcgac cgtgtcagcg acgttgcccg cacactatgc tgcgcccgtt
      781 cctctgttgg gcgctggatt aactagttca cacagtcggg tgttgaggga ctgaaatcat
      841 tacctgccga gcgagcccgc cgctggccgt ttgcgcatat ctacacacta ttacgtgaac
      901 aggtaaaata ttcactcgcg actttggcta caattattca cgctggagta cagaactgct
      961 gacaataaaa atcaatgaga taaccggctg cctgttacat gctggaacca ttcgccgctg
     1021 gttgtcgtct gtggggcttg tgtggcgaag ggctgcgcca actctacgta tccgtgcccc
     1081 gcctaaagat aaaaagatgg caacaatcca taacgcactg gacgaatgca gcacagagca
     1141 tccggtcttt tatgaagatg aagtatttat ccatcttaat cccaaaatcg gtgcggactg
     1201 gaaactgctc gggaagcaaa aacgaggggt cacgccggaa caaaatgaaa aatattctct
     1261 ggacgtagcg ctgcacagcg ggacaggtta agtcagctgt gtgggcgcca acagcaaaag
     1321 ttcggcgctg ttcatcagac tgctgaagcg gcttaaagct acatactgtc ggacgaaaac
     1381 catcacgctg ctcgaggaca actacattat ccacaaaggc cgggaaaccc agcgctggct
     1441 gaaggataaa ccgaagtgca gagtcattta tcagccggtt taatctccat gggtgaatca
     1501 ttttgaatag ctatggcaga tacttcacgc cacaataacg cgtaatcatc agtgccgctc
     1561 aatgtggcaa ctattgaaaa agttcgccat tttatggaat ccgtcagtcc attccccgaa
     1621 ggcaaacatg ggctggcaaa aatgtgcgat attaggcgca gctatttagt tattccgtca
     1681 tttctcccgc ccgtgtaaac actactgaat cttgtcgttg tgacaaagct ataaatagtc
     1741 aatcacagca cgtttctgcg tacgacgcgc tgcaattttc tttacgattt ataactgtac
     1801 aggtgaacta tgagttattt ggatacatac atatagtgta acgcctgttg tatattttac
     1861 tcatatcttt ttacaggatg aaaaccaatg atactggcgc atagcctcct ccaaccttac
     1921 ccctgtataa cacgactcat ataacctggg atttcatttc gtagttttac aatatcaaca
     1981 cccggggcga tatgtttctg ataaaacttg tctgcaagca cgtttgctat gtaatctgtt
     2041 gtactgtttt ttacagctcg gctcatacca tcgcaaagag catccatttt tttgccaaaa
     2101 gaagcatttc ctattattat atgattaata gaactttgtg ccgctttaac atcaagagtt
     2161 agttcaaact ccttacttac aagagaaaag accattttcc tctcttcaga agaaattttt
     2221 gcattttttt gaaggcagca attatcaatc tcttggttga taacccttga cggatatact
     2281 gtccgttcat cacttaccca tccatgaata tctttagtac tctgcctgct taccgtctcc
     2341 agtatacagc cgctaaattt gttaacatta aagcggatag agcgctctcc cattgaacac
     2401 ttcacgacct catcattctt cccacggatg gtgccccgga aagaattatc tttatgataa
     2461 ctgataccaa aagagctgaa ggacacacct gtcgcattta ctggcataac aaatcctttt
     2521 cattaaaatt tgcttacaaa aattgtatac aatcatttta attaaaaaaa tacatgtgta
     2581 aatagcattc gttcaaaatt taattttttg tttgaatcct atcacatatt tagtatcttg
     2641 actcctgtca ccaatatggt tcaataacat agactaaaga gggagaaaat atgcctgtca
     2701 tattaaactt ttcgagtgaa agagtattgt ctgaaagtga actggaggct ttacgtcatg
     2761 tcggaagagt tagtcagagt gaacagcttg ttgtgagagg ccgcactatg agacttcatc
     2821 atatttcatt tatggacagt tttagcgtgg aaccggtttc cggcggactg ttggatcgcc
     2881 ttagtgcaag agggcatcgc cttctcgcag aaaatctgga gatacagctt aacagaggcc
     2941 atactttttt gcaggcattc cgtttatata tggaacagag ccgtgcaacc ccatgtacgc
     3001 gccaaaacgt cagtagtgca atacaaaaca aaattaactc tcatgcattt actgttagtc
     3061 atcaagactt ttcctgtcat gaacaacacc tgaattgccc gataacatta tgcattcctg
     3121 aaactggtgt ttttgtcaga aatgcaaaaa actcagaaat atgttcatta tatgaccaca
     3181 atgcactcac tgaacttatc cgacgcaacg ctccacaccc tctcagccgt gaaccctttg
     3241 ttccggaaat gattgtcagc aaagatgaat gccattttaa tctaatagaa caatattttt
     3301 gcatattagc cacacaaaat atatgcacta gaatttgact gtatggttcg atttaaatgt
     3361 tgcaaaatta attttactag ttattgccat ctatttcata ctccctaatt cttgatacaa
     3421 tctgtct
//
LOCUS       DABSYT020000221         3416 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.223, whole genome shotgun sequence.
ACCESSION   DABSYT020000221 DABSYT020000000
VERSION     DABSYT020000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.223"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            156..1514
                     /gene="dgcT"
                     /locus_tag="ID725_RS24805"
                     /old_locus_tag="pgaptmp_004961"
     CDS             156..1514
                     /gene="dgcT"
                     /locus_tag="ID725_RS24805"
                     /old_locus_tag="pgaptmp_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGV7853363.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            1612..3222
                     /locus_tag="ID725_RS24810"
                     /old_locus_tag="pgaptmp_004962"
     CDS             1612..3222
                     /locus_tag="ID725_RS24810"
                     /old_locus_tag="pgaptmp_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGV7853364.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLSFILY
                     FLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNI
                     SPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVAD
                     VLHIKNAISDKFNIVLEITERNLVEDTDHALQKINELRSHGCEFAVDDFGTGYCSLGL
                     LQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTSHQAEYLI
                     HNNVTLVQGYLYAKPMKATEFYQWYVNR"
     gene            complement(3324..>3416)
                     /locus_tag="ID725_RS24815"
                     /old_locus_tag="pgaptmp_004963"
     CDS             complement(3324..>3416)
                     /locus_tag="ID725_RS24815"
                     /old_locus_tag="pgaptmp_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HGV7853365.1"
                     /translation="TSINFYVTRQNDYNYVFSTGRTPRFICISH"
BASE COUNT         1044 a          556 c          711 g         1105 t
ORIGIN      
        1 aaacaatgta taaagcactt aggcaataat aattacattc agcaactatc atcatcggta
       61 ttgtttgtgg gcggaatttc atattgacaa cagtacagtt cttatatcta ttaataatag
      121 aaagggatct acaacctaca gattggtgta gctttatgga aaaagactat ttgagaatta
      181 gtagtactgt attagtgagc ttattgtttg ggcttgcttt ggttttggtg aatagttggt
      241 ttaatcagcc aggcgttgag gaagttgttc cacgatcaac ctatctgatg gtaatgattg
      301 ctttattttt tatcgatact gttgcattta tttttatgca gttgtatttc atttatgacc
      361 gtaggcaatt ttcaaactgt atacttagtt tggcttttct gagttgtttg atttactttg
      421 ttaaaaccgt cattatcatt cagcaaatta ttgaggggcg tttgacaagc agtgttgtcc
      481 aaaatgacat tgcaatctat tatttgtttc gtcagatgag tttgtgcata ttaatatttc
      541 tggcattggt gaataaagtt agtgaaaaca caaaacagcg caatttattt tccaaaaaaa
      601 tgactttgtg cataagtttg ttttttgttt ttgggggggc gatagttgct cacatacttt
      661 ctagtcatta tgagagctac aatttacaca ttgccgagtt aaccaatgaa aatggtcagg
      721 tggtttggaa agcctcatat gttaccataa tgattttcat gtggttaacc ttgctttcgg
      781 ttaatctata ctttaatggt ttacgctatg atatctggaa tggagtgacg gtgattgctt
      841 tttgtgccgt actgtacaat atctctctgt tatttatgag taggtatagc gtgtccactt
      901 ggtatattag tcgcactatt gaagtagtca gtaagttaac cgttatggta atatttatgt
      961 gccatatatt cagtgcgcta cgagtaacaa agaacattgc acatcgcgat cccttaacca
     1021 atatatttaa cagaaattat ttttttaatg aactgacagt tcaatcagca tcagccaaaa
     1081 aaacgcctta ttgcgtcatg attatggata tcgaccactt caaaaaagtc aacgacacct
     1141 gggggcatcc ggttggcgat caggtgataa aaacagtggt gaatatcatt gggaaaagca
     1201 tacgaccaga tgacctttta gcgcgcgtcg gcggcgaaga gtttggcgtc ttgctgacgg
     1261 acatcgatac tgaacgtgcg aaagctttag cggaaaggat tcgggaaaat gttgagcgtt
     1321 taactggcga taatcctgaa tacgctatac cacaaaaagt gacgattagt attggcgctg
     1381 ttgtgactca gggaaatgcg ttaaacccga atgagatcta tcgactggct gataacgcac
     1441 tatacgaggc gaaagagacc gggcgtaata aggtggttgt gagggatgtg gtgaattttt
     1501 gtgagtcacc ataaagcggc attttagcgg gtgattttgg taagcgctaa atagctattc
     1561 gcagacatgc gtaacaattt tcagaaatat catagtacta tatgatctta aatgcttatc
     1621 attgatcagt ctgctatcac ccgcttttac tggcaattgt actataattt cttaggttgc
     1681 atcaccctga tgcgtgggga aacatgtatg gtattcaaca aaaaaatgtt tgtactcatt
     1741 ataataccag gcattctggg ggtgttactg tcgtttgcta tgtcagtatt tcaaatgaat
     1801 cgagatacga caattaccgc cggtattctg ttaaaacagc ttgataatgt tacgcaaatt
     1861 gtcaaacata cgacaaagct tacttctatt ctggtgatga aaccctgcaa agatatcctg
     1921 gaacaattga ttgctaacgg agcattgacc ccctatgttc gaacaacggg tcttatagaa
     1981 aataatttcc aaatatgttc ttcagttagc ggtttcaaaa aaatgaatgt taacgatgtt
     2041 tacggtacga gctttcacaa taaaaataaa gaatcacgca tagtatcaat tagcggtaca
     2101 agttttgtac caggtaaaac agccatagtg ttccttatgc caatagggaa tgatatgaca
     2161 gcttttagta ttgtagagtc acgatatatc tatgatctga tggatgttct tgatgatgag
     2221 aatgatgatt cattttctct tcgctttact gaagggcctg caattattag tggtgtaaat
     2281 aataatgaca ggttatatat gttaaaaaaa gattttaatt ctgcgatcag tcaggctagt
     2341 cttacagtta ctacgccgat gatttcatta tatccctatg tcattagtaa tgtattttac
     2401 attcttccat tatctattct tctttctttt atcctgtact tcttatggca gcgctggata
     2461 tcacggaaaa tgtccctggc agaagaaatt aagaaaggaa tgtcatccgg agaattttct
     2521 gtgcattatc aaccagtatg tgacacgaca accaaagcgt gtctgggggt tgaggcgtta
     2581 atgcgttggc aacgcgaaga tggtaaaaat atatctccgg tagtttttat ccgcgctgca
     2641 gaagaggaaa atttaattat accgttaact aagcatctct ttgaactgat tattcaggat
     2701 gtacaaagct ggaaagtgaa aaagccgttt caccttggga taaatattgc cgctgaacat
     2761 cttgcacatc ctgattttgt tgctgatgtt ttacatataa aaaatgcaat atctgataaa
     2821 tttaacattg tccttgagat taccgaacgc aatctggttg aagatactga tcatgcatta
     2881 cagaaaataa atgaattaag gagccatggc tgtgaatttg cagttgatga ttttggcacg
     2941 ggatattgtt cccttggcct tctgcaaaag ttgtctgttg attatcttaa aatagataaa
     3001 agctttatag acacgctcac tacagcggaa gacgaaacgc ctgttttaga tattattatt
     3061 aagctgagta atcgactcaa tttgataacg attgcagaag gtgtcagtac ctctcaccag
     3121 gcggaatatc ttattcataa taacgtgaca ttagttcagg gatacttata tgcaaaacca
     3181 atgaaagcga cagagtttta tcaatggtat gtaaacagat aaataattaa ccaatattgg
     3241 aatggttttt tattgcctta aaatataaac aaagccatca gaaggttatt aactggtgac
     3301 aaacgccagg agcaacaatg ctgttagtgg gaaatacata tgaagcgtgg tgtcctgcct
     3361 gtagagaata cgtagttgta atcattttga cgtgtaacat aaaaattaat tgaggt
//
LOCUS       DABSYT020000222         3341 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.224, whole genome shotgun sequence.
ACCESSION   DABSYT020000222 DABSYT020000000
VERSION     DABSYT020000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.224"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..910
                     /locus_tag="ID725_RS24820"
                     /old_locus_tag="pgaptmp_004964"
     CDS             <1..910
                     /locus_tag="ID725_RS24820"
                     /old_locus_tag="pgaptmp_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HGV7853366.1"
                     /translation="NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKR
                     RDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSS
                     CQPGKHQYKNARQEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLF
                     ARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYR
                     IKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPH
                     HYNGGLTPEESENRYHFYCKTVASIT"
     gene            919..1071
                     /locus_tag="ID725_RS24825"
                     /old_locus_tag="pgaptmp_004965"
                     /pseudo
     CDS             919..1071
                     /locus_tag="ID725_RS24825"
                     /old_locus_tag="pgaptmp_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            1161..1535
                     /locus_tag="ID725_RS24830"
                     /old_locus_tag="pgaptmp_004966"
     CDS             1161..1535
                     /locus_tag="ID725_RS24830"
                     /old_locus_tag="pgaptmp_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGV7853367.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            1532..2023
                     /locus_tag="ID725_RS24835"
                     /old_locus_tag="pgaptmp_004967"
     CDS             1532..2023
                     /locus_tag="ID725_RS24835"
                     /old_locus_tag="pgaptmp_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGV7853368.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFTALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGLPT
                     FNW"
     gene            2035..2232
                     /locus_tag="ID725_RS24840"
                     /old_locus_tag="pgaptmp_004968"
     CDS             2035..2232
                     /locus_tag="ID725_RS24840"
                     /old_locus_tag="pgaptmp_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGV7853369.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            2317..2658
                     /locus_tag="ID725_RS24845"
                     /old_locus_tag="pgaptmp_004969"
     CDS             2317..2658
                     /locus_tag="ID725_RS24845"
                     /old_locus_tag="pgaptmp_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853370.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            2719..2862
                     /locus_tag="ID725_RS24850"
                     /old_locus_tag="pgaptmp_004970"
     CDS             2719..2862
                     /locus_tag="ID725_RS24850"
                     /old_locus_tag="pgaptmp_004970"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853371.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            2856..3275
                     /locus_tag="ID725_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /pseudo
     CDS             2856..3275
                     /locus_tag="ID725_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT          888 a          864 c          830 g          759 t
ORIGIN      
        1 caaccgcgct cttgatgtcc gactcgctga acggttcacg atagttgcca gactgagtga
       61 cagccactca gttgtcagcc tctgttctgc tctggaaata caccgcagca gttaccagta
      121 ctggcgaaaa cgacgcgata cggttaatcc ggcgcgagtc aggttgtgca gcgaaatacg
      181 ccgggcgtgg aaccaaagta ggggctctgc gggggcgcgc acgctggctg aaatgctgac
      241 ccaaaacggc gtcccgatga gccgttaccg tgccgggcgt ctgatgaaat atctgaacct
      301 gagcagttgt cagcccggaa aacatcagta caaaaatgct cgtcaggaac atacctgcct
      361 gccgaatctg cttgagcgcc agttcgctgt gcccgagcca gatagggtat ggtgcggaga
      421 tattacgtat atctgggcag gaaatcgctg gtgctatctg gcggtcgtca tggatctttt
      481 tgcccgcagg gttatcggct ggagcctgtc agcgaatgcc gatactgccc tgataagcag
      541 tgccctgcgg atggcgtatg aggtgcgtgg tcagccgcgg gacgtcatgt tccatagcga
      601 ccagggaagt caatatacag gactgaaata tcaacaactt ctctggcgtt acaggataaa
      661 gcaaagtgtc agccgacggg gaaactgctg ggataacagt ccaatggaac gcttcttccg
      721 cagtctgaaa acagaatggg tgccaaccga tggctacaca ggcaaagacg tggcccggca
      781 gcaaatcagc agttatattc tgaattatta caatagcgtc aggcctcacc attataacgg
      841 agggctgacg ccggaagagt cagagaacag atatcatttt tactgtaaaa ccgtggccag
      901 tattacttga ccactacact ggaactcatg ctcaccagcg gtgagctgaa tccccgccat
      961 cagcataccg tcacgctgta tgcaaaaggg ctgacctgca aagccgatac cctcggcagt
     1021 cgaggttacg tttatatggc tgtttatccg acgcccgaaa cgaaaaagta aatcatcaga
     1081 ataaccttct gctccggcct ggtgttttca ccacgccact tccccatttc tcatctctgc
     1141 atatcaggaa aaactttagt atgaaaacat tacccgatac acacgtacgg gaggcatcgc
     1201 gctgcccgtc tcccatcacc atctggcaga cactgcttgg ccgtctgctg gaccagcact
     1261 acggcctcac gctgaatgac acaccgttcg ctgatgaacg tgtgattgag cagcatattg
     1321 aggcaggtat ttcactgtgt gatgcggtga actttctcgt ggaaaaatac gtgctggtgc
     1381 gtaccgacca gccgggattc agcgcctgta cccgctctca gttaataaac agcatcgata
     1441 tcctccgggc tcgcagggcg accggcctga tgacccgcga caattacaga acggtaaata
     1501 acattaccct gggtaagcat ccggaggcga aatgatgaaa ctggccctga cactggaagc
     1561 cgacagcgtt aacgtacagg cactgaatat ggggcgaatt gtcgttgaca ttgatggtgt
     1621 taatctcgct gaactgatta acatggtctg cgataacggc tactcccttc gtgttgttga
     1681 tgaatctgac cggacctcag cagactgcac gccaccattt actgccctta ccggcatacg
     1741 ctgcagtacc gcacatatca cggaaacgga caacgcctgg ctgtactcgc tgtcacacca
     1801 gaccagtgac ttcggtgaat cagaatggat tcattttacc ggtaacggct atctgttgcg
     1861 taccgatgcg tggtcgtacc cggttctgcg gcttaaacgc ctgggtctgt caaaaacgtt
     1921 ccgtcgtctg gtcgtcacac tcatccggcg ttatggtgtc agtctcattc atctggatgc
     1981 cagtgccgga tgtctgccag gtttacccac ttttaattgg taactaggaa caacatgaca
     2041 tcattaaccc cggaagcagc actggatatt ctgattgcgt ggctgcagga caatatcgac
     2101 agcgaatccg gaattatctt cgacaacgat gaggataaaa cggattcggc agcattgttg
     2161 ccctgtatcg aacaggtcag ggaagatgtc cgtaccctgc gccaactgca gcttctgcaa
     2221 cagaaccggt gagtctcact caccatactt cattccactg acgccagcct gaacagggct
     2281 ggcgttttcg tttataaacc aaaaggaaaa ccgataatgt caggcatcat aatctcccgt
     2341 ccggaagtgg acaccgggca tacggacgtt atctgctcca ccagtatccg acacgttgtg
     2401 acggtacgga atgcagcact gcaacaaacc gaaacgctta tccggcaact ggcagaaatc
     2461 tcagtcctga cagcagcaga cattggcggc aaaaccgccc tggaccgggc gatgaaacag
     2521 gattttcact gcggctgctg gctgatggag aaacctgaaa ccgcaatgaa agccatcacc
     2581 cgcaacctcg actgcgaaat ctggcgtgac ctgatgcaac gctccgggat gctgagtctg
     2641 atggatgcac agggctagga tacatggtac cgctcactgg agtacgataa tattccggaa
     2701 atcagcgagg cgaatatcat gaatgcattc aaacaactgc accagagtaa ggatgaagtg
     2761 tttgagcgtg gggtgatcaa cgtcttcaga gggccgaacg gaattacaaa accaattccc
     2821 cctgtaaatt cggcagtaaa attatcgtca acaacttggt gagatgggac agatggagat
     2881 ttcatcttat cagtggaatg caggcagatc gcttagctga cctggaaaga atgttgcatc
     2941 tgctcagcgg taaaccgctc cccaacaacc gagggaatat caccattaat ctggatgacc
     3001 acctacagtc cgttcagggt aaagagcgct atgaagacaa gtaaatactt taagaagggc
     3061 ccgcacacat cacattcaga aagccagagc tggttgacag gcttaatgat attattgcca
     3121 gacactacct aggaatgtta ccttcacaaa aatctctaag aattgattca attgtgtatt
     3181 ttagcctcat tccctcatgt aaagtcattt gggggttatt atgggggtca atcatttatc
     3241 caacacgcat taatcattta aaatcaatgc attaataatc atattcgact cctgtgatct
     3301 ccgccaaact tcctcttctg aactctcccg acgtcaacaa a
//
LOCUS       DABSYT020000223         3336 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.225, whole genome shotgun sequence.
ACCESSION   DABSYT020000223 DABSYT020000000
VERSION     DABSYT020000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.225"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..750)
                     /locus_tag="ID725_RS24860"
                     /old_locus_tag="pgaptmp_004972"
     CDS             complement(<1..750)
                     /locus_tag="ID725_RS24860"
                     /old_locus_tag="pgaptmp_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HGV7853372.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKS"
     gene            complement(760..1440)
                     /locus_tag="ID725_RS24865"
                     /old_locus_tag="pgaptmp_004973"
     CDS             complement(760..1440)
                     /locus_tag="ID725_RS24865"
                     /old_locus_tag="pgaptmp_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGV7853373.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1427..1894)
                     /locus_tag="ID725_RS24870"
                     /old_locus_tag="pgaptmp_004974"
     CDS             complement(1427..1894)
                     /locus_tag="ID725_RS24870"
                     /old_locus_tag="pgaptmp_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HGV7853374.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(1894..2430)
                     /locus_tag="ID725_RS24875"
                     /old_locus_tag="pgaptmp_004975"
                     /pseudo
     CDS             complement(1894..2430)
                     /locus_tag="ID725_RS24875"
                     /old_locus_tag="pgaptmp_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2494..3141
                     /locus_tag="ID725_RS24880"
                     /old_locus_tag="pgaptmp_004976"
     CDS             2494..3141
                     /locus_tag="ID725_RS24880"
                     /old_locus_tag="pgaptmp_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGV7853375.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT          897 a          817 c          798 g          824 t
ORIGIN      
        1 actttttgcc accatataga cagacattag gataaagact acagatgata tttcagcaaa
       61 tgatagaact aaatatgacg gctttgactt aaactcaaaa tctccggcct gattgctgcg
      121 gattgtctct gccagcctgc ctcggtcaat ctcacgacca gccatcttgt cctgaacatt
      181 gaagtagtca atcggaccga gagcagccat cccaaggtga taaacaaact caccaaaccc
      241 tgaaggattc tgctgataca tctgagcaac gttgttaggg tcaacaccga cgcgcgccag
      301 ttccttggcg ttgttttgca gccatgattg cattgcttct ggagacgagg ccgcaaggcg
      361 tgcgccagcc gctaaggtgc cgatagaatt gcgctggtct tcgtctgccc acttcatacc
      421 agactgaatc ttctctaatt gaccaggata tttggtcatc agatctcgaa cctgctgtcg
      481 atcgccggac tggatggctg ccgcatattc tttttggaat gcagcatccg cttcctgttg
      541 ctttgcggct tgagatgttt gagcgacact accaagtccc tgcaacgcct gaaggccgat
      601 gttattgcga cctgaacgct ccatttcgtt gttctggcga atataggcca acgcctcact
      661 tacatcactt gcctttggcg catttgagtt ttgcccaccg ataccagcaa gaaaaccgcc
      721 tgagttgatt ccttgttgcc aagtagccat attcccacct taaaacaatg atccaagacc
      781 accgataaca ccgccagcaa gagcaccaat accagtccca ataacaggaa ctgcactacc
      841 aatcattgca cctgatgcgg ccccaccaat ggcacctgta gcaaaactct gtaggcctga
      901 aggcttattc gcatttgctg ccgatgctgc cgcctgctgt tgatacaatt ggctgacatt
      961 gttagcgtag ttctgtccgg cgtttgcctg acctgtaaga gcaccaaggc cgatatttgc
     1021 cagattgttg tagttgttca tctgacctga cagccagttt tgaccgagtg taggtgcgat
     1081 tgctgctaac tggtttcctg ttgctgtaga gcctaatcca cccgttgcct ctgctgctgc
     1141 cagactctga tagcgcgcct gccctgcaag gtctttatac tgctgagagt tgtaatactg
     1201 gttaagcgcc tgaccttgcc cctgaagaga ggaaagattc tgcaactgtg atacgtactg
     1261 ctgagcgagt ggcgtgaacg gtgcaaggtt ttgcatgttc gtctgccaca tttcacgctg
     1321 cagttcgatg cccttttcag ttgcgcgtgc ctgggcttta gatccggagt cgctgccgcc
     1381 tttcatatac ccattcatgg gaagcaattt attcttgaag ctttcgctaa gtactaacat
     1441 ttaatagctc ctcatatttc gaacgaggta attgatagag ggtgattcct accggttttc
     1501 cgttactcat gtacgcatca tcaaggtgac caacacgggt agcgccaagc aaacggataa
     1561 ttgcccgtcc gtatttggtg gtgtcaggaa ccatggtgat gctgttaagg aatggtgagt
     1621 tttcgagaag ccatttgcag aataatcgat gcccttgtag tgcatattcg ccacggaatc
     1681 cggggtcata caccgcatgg cattcaacaa cgctatgcca gaagttacgc acttcatgaa
     1741 ctccagccag cactaatcct tcgtagatgc cgagatatac cgcatcaggc ttgatgtagt
     1801 atttatctcc actgtctacg atattccccg tgtttaccgg gttgttaagg aattctgcaa
     1861 gtttcaccgg gttatcgatg agctttattt ccatcactgc tccgcaatga ttttgatggt
     1921 tgtggcagta aacgccgcgc cattcgactg aatggttaac gtgctgccat ttgtggcaag
     1981 aaagccgtct ttatccacgc tgaagaacgt agctaacagg atgttgtcgg ttgttgttgc
     2041 cgagttgcga ctgcttacta gcgtgtcagg aacagaaccg gagaatgtta gctgcattga
     2101 cctgttggcg gttccgctgg gccacgtccc gacaatcgac agcttgaaga acaaggtttt
     2161 gttctcgttg aacacaacca tcttgttgtt aacggtgtcg aagaatggtg ccaacgtccc
     2221 ggatgacggc gtaagcgttt tcagcaggct aacaaggttg gtcggcgctg tcgggatggt
     2281 tactgatacg ccagagtaaa caacctctga cttcttgcgc gtagtggcat actccagagc
     2341 attaatgcgc gtttcatggt ctgaaacctg cgattccagc gactgaactc tggtatcaag
     2401 cgatgcaata tcgctttcat tcaaggcaat cagtgggttt acgtcatgga gtgggctcaa
     2461 caccagtcag agccgcatgt acacgcaata atcatggtga acgaaagaag cattaatggg
     2521 caagctggca aaattgtcag caaatgggta acactggcaa acaatgcaat cccatcaaat
     2581 gttcctcccc agtgggatct agatttgaaa ggtcagcaat attcagagcc tacaaagtgt
     2641 ataggtaaac agattgatta catggcaaag cccgaaacta agttaaatga ctttaataga
     2701 gaggcgagca agagcctata tgattggtca gatgccaacg tttggggtta ttcgaatggc
     2761 tgggcgcggc atgagataaa aaaagaaaca ctttctatca ctggctttca tgctgtaaga
     2821 cgcatactaa aagccgttca ggcatcaagg aaagacaact acaaagctaa gcgacagata
     2881 cagcgtacat tgaaacgaca agacataaat cgatcaccaa tcaccccgtt ccaagcaccg
     2941 ttaattagca aacacgattg ggatttatca gtgatagcgg tcaccaaaga ggcagagaca
     3001 gagcgcaggg aattactatc ctcacagata ataaactcga acaaagtaca aacccaccca
     3061 ctgttaagga tgttaagaag agcacgagtg agagcatcca gagccaaaag agaaagcgga
     3121 ggttatgacc attactacta gtagtgacag atcgtgacaa ggcggtgcaa aaaacgacca
     3181 ataataagag agatatgcct gccaaaccgc caaaagcgcg gagcgcggca aacgccgcga
     3241 gaggcgcaag aaggcgaacc tatccattga aacggtcaga agcgcatcgc gctcgacgac
     3301 cgaagtcccc actaacaccg ttaacgaaag atcaaa
//
LOCUS       DABSYT020000224         3326 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.226, whole genome shotgun sequence.
ACCESSION   DABSYT020000224 DABSYT020000000
VERSION     DABSYT020000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.226"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..384
                     /locus_tag="ID725_RS24885"
                     /old_locus_tag="pgaptmp_004977"
     CDS             <1..384
                     /locus_tag="ID725_RS24885"
                     /old_locus_tag="pgaptmp_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGV7853376.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
     gene            418..1134
                     /locus_tag="ID725_RS24890"
                     /old_locus_tag="pgaptmp_004978"
     CDS             418..1134
                     /locus_tag="ID725_RS24890"
                     /old_locus_tag="pgaptmp_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGV7853377.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            1131..1448
                     /locus_tag="ID725_RS24895"
                     /old_locus_tag="pgaptmp_004979"
     CDS             1131..1448
                     /locus_tag="ID725_RS24895"
                     /old_locus_tag="pgaptmp_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGV7853378.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            1445..1747
                     /locus_tag="ID725_RS24900"
                     /old_locus_tag="pgaptmp_004980"
     CDS             1445..1747
                     /locus_tag="ID725_RS24900"
                     /old_locus_tag="pgaptmp_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGV7853379.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            1737..2054
                     /locus_tag="ID725_RS24905"
                     /old_locus_tag="pgaptmp_004981"
     CDS             1737..2054
                     /locus_tag="ID725_RS24905"
                     /old_locus_tag="pgaptmp_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGV7853380.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            2008..2325
                     /locus_tag="ID725_RS24910"
                     /old_locus_tag="pgaptmp_004982"
     CDS             2008..2325
                     /locus_tag="ID725_RS24910"
                     /old_locus_tag="pgaptmp_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853381.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            2312..2749
                     /locus_tag="ID725_RS24915"
                     /old_locus_tag="pgaptmp_004983"
     CDS             2312..2749
                     /locus_tag="ID725_RS24915"
                     /old_locus_tag="pgaptmp_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGV7853382.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            2751..2942
                     /locus_tag="ID725_RS24920"
                     /old_locus_tag="pgaptmp_004984"
     CDS             2751..2942
                     /locus_tag="ID725_RS24920"
                     /old_locus_tag="pgaptmp_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853383.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            2945..>3326
                     /locus_tag="ID725_RS24925"
                     /old_locus_tag="pgaptmp_004985"
     CDS             2945..>3326
                     /locus_tag="ID725_RS24925"
                     /old_locus_tag="pgaptmp_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853384.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPD"
BASE COUNT          920 a          693 c          992 g          721 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgtgacaga
      181 gggattgagc ggcagatgac ggaaggtgag ttaaaacgac ttgcagaacg gcaactgacg
      241 aaatgggcaa agcaagttgg taacgggatg agtattcccc cgatccggag acaactcgca
      301 tcgccgaagt gcccgcaagg gccaacgcca attgaattac tgaaacagga atacgagcgc
      361 cggaaggcgg ctggttttgt ttgaatctga gaaacgattt tgtcggagga aattttaatg
      421 gaaaccgtat ctgacgcact gaaagcactg aaaagagcct cttcacatgt ggtggcagct
      481 cgccttggaa ttagtcgtga agaggctgtc aacgaactgt ggaaactgaa acgccgtgga
      541 gaagcggata acaagggggc gatatggtgg ctgactcagg ctggtgaaag tgaaccggtg
      601 tcaccggtac cgaaagtgac ggcgcaaatg ctgacagagg cgattgaaca acatggccca
      661 caaacggcgg atgaactggc attgatgttc ggaattacct cccgccgggc gaattcatcg
      721 ctggcaatgg caatcagcaa agggtgtctg attcgcgtga atcaggatgg taaatttcgt
      781 tactgcatac cgggcgttga tttaccggca gagccgaaag cagcatccgt agctgaaacg
      841 gagggtaaag cccttcctca gccagctggt gttgcgttac cagtccagga aacggctgca
      901 caggaagaaa ttaaaacaga agcggtggag gacattgtga agttgcagca atcgttcact
      961 gaagcgaaag cagatgacct gattctacca tcgctgcatg tggctaaccg cgagctgcgc
     1021 cgggcgaaaa gtaatgttca gaagtgggag cgagtctgtg ctgcgctacg ggaactgaac
     1081 aaacacaggg atattctccg ggatattacc gccaccagag agcagcagcg gtgagtggct
     1141 ggaagaagtg gcgctgggct gaaatcctga tactccggca gtgtgcggga acgatgagag
     1201 tcgaaagcat cggttatctg attggccgta gtgagtcagc cgtcaggacg aaagcgcggg
     1261 aactgggtat cagcatgatg ttacggggtg attatcacca gtcagccaaa tgttcacagc
     1321 gtgatattga gctggcgtgg caactgcatc agcgtggtgt accccgacgg gaaattgccg
     1381 aaaagtttgg gatgaagttg ggcgcagtga ataactacgt ttatttcgac aggagggttc
     1441 aggagtgagg gtgagggttt atatcgccgg tccgatgacg ggatatgaaa attttaaccg
     1501 cgaggcattt cacaaaacgg aagaggtgct gaaacgggaa gggcataccg ttttaaaccc
     1561 ggcagtactt ccggacgggc tgactcagcc acactacatg gatatttgca tggcaatgct
     1621 ccgttgcgtg gatgcggttt acatgctgaa aggctggcag cagtcggcag gtgcaggggc
     1681 tgagctggca ctggaggaga aaccgggcca tgcggtgatt tttcaggagg tgggcagtga
     1741 atattgaccc ggcgataacg attgatatgg ccctgaacgc cggcctggca cttcttggtt
     1801 atttctacat tatgttctgc agcggacgat ggctgtcact gttgttcatg aaaaaatgga
     1861 ataaacgccg taagcaggag caacgccaga aggcaatgga tgcatttttc gaagccttcg
     1921 gaattgacgg catggaacca ggggatccag ctcgcgcaat cagcagaggg ggtgtagtaa
     1981 tccttgtata tcggagtgaa gagaaaaatg acgatcacaa aacaacgtgt agaagaaatc
     2041 atatcccgca ttgaaatgta tggacatggt gcagggtata tcgcagacga ggttaatgat
     2101 ctggctatac tggcgctgaa tttatcaaat atcgcgaacc tgaagcgata cgagcttgat
     2161 atgggaggtt gcgactcgtg cggtcaggat tgtggcgctg atatgactga agatcctgat
     2221 ggtgattatg tcctgtttga tgacgtggtt aagttgtttg aatttgatac aaccactcaa
     2281 aagttagaaa tcccggcaaa ggaggcagcc agtgggcaag attgactatc aggcactgcg
     2341 tgaggcagca caaaactatc aatcgacgct ggcgtggtat caggctaccc cggacagccc
     2401 aaatgctgaa cgggattgtg atgcggctct tgctgcgttt aagcgtcaca tccgtcatcg
     2461 ggaagcggat attatcgctg atttgctgga tggactggaa gaagcaaaat cacaactcaa
     2521 cgagcagcgt gagtattacg aaggcgttat ctctgatggg agcaagcgta ttgctgaact
     2581 ggaagcgcgg gaagttcaat taccgactcg ctacgacctt cgatatggac acccgataaa
     2641 tgcagatgag cgacatgtca tgatacctaa agaaaatggc agttggcttt acctgattga
     2701 cctagaacac gcattacgcg tcgctggcat tcgcatcaaa ggagagtgag atgaacggac
     2761 aaatatcaat tgttcgacca ggagcatgtg acgatcgcga aatacgaatg attattcgtc
     2821 tggcgatggg gaaaacaata actgctctca ttactccaga aaatctcgca ttagcattaa
     2881 caggaaagtc agacatgcca gtagagctaa agctgcgaaa tgttgagatt aaggtgaaat
     2941 agctatgacc actattacca aagatcgact gctgacaatc cagcattggc gcgaaacata
     3001 cggaccgggt agcaacgttg tgcttccagc agaagaagcg gaagagctgg cacggattgc
     3061 gctggcatca ctggcagcag tatcggatga acgagcagcc tatgaattat ttatggagaa
     3121 gcgtttcgga gaatctgtag atcgccgcag agcaaaaaat ggcgatagag attacatggt
     3181 atgggatatg gcgcttggct ggattatctg gtgtcaccgc gccgccatgc ttcaggctgg
     3241 aaactttcgg gaaaataagg gttcgtcaac caataatttt cgggaaatct cggaaacgtc
     3301 aaccaactat ccggtaactc cggatg
//
LOCUS       DABSYT020000225         3319 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.227, whole genome shotgun sequence.
ACCESSION   DABSYT020000225 DABSYT020000000
VERSION     DABSYT020000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.227"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            550..741
                     /locus_tag="ID725_RS24930"
                     /old_locus_tag="pgaptmp_004986"
     CDS             550..741
                     /locus_tag="ID725_RS24930"
                     /old_locus_tag="pgaptmp_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGV7853385.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(794..1027)
                     /locus_tag="ID725_RS24935"
                     /old_locus_tag="pgaptmp_004987"
     CDS             complement(794..1027)
                     /locus_tag="ID725_RS24935"
                     /old_locus_tag="pgaptmp_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853386.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1123..1746)
                     /locus_tag="ID725_RS24940"
                     /old_locus_tag="pgaptmp_004988"
     CDS             complement(1123..1746)
                     /locus_tag="ID725_RS24940"
                     /old_locus_tag="pgaptmp_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGV7853387.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1835..2344)
                     /locus_tag="ID725_RS24945"
                     /old_locus_tag="pgaptmp_004989"
     CDS             complement(1835..2344)
                     /locus_tag="ID725_RS24945"
                     /old_locus_tag="pgaptmp_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853388.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            2802..3260
                     /locus_tag="ID725_RS24950"
                     /old_locus_tag="pgaptmp_004990"
     CDS             2802..3260
                     /locus_tag="ID725_RS24950"
                     /old_locus_tag="pgaptmp_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853389.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT          999 a          789 c          660 g          871 t
ORIGIN      
        1 agcctcctga cgatacctcc caccatgttt tgcgatggca cgctaccgcc cttccggtat
       61 ttacccgcat caaccggtac cagaatgctg agtaacaaac cagctagaat tgacttaatt
      121 ttgatcatta tagaataatg atctgaacta cagcaacatg acagatttat cgatcgatat
      181 aacacaaatg aacagttctt tattctgaca aatatcgcaa aaacagcaca ttacaaaatt
      241 tataaccata attactggaa aagtgcagaa aattattcca ctgaatgctt ttaatacagt
      301 tggttagctt aaaaaatagc aatttttgat ttcagtcatt gaaagttgca atgtttgacg
      361 attcaacagc atttaagtga gtttcaggat atgggaagga catgaaagga ggagcgcaat
      421 aaaatacaca ggaagaaggg gaaaatgttt cgtaaaaaca gaaaattaac agcgcacatc
      481 aggaaacgca gtctcctgat tcagtgctac cacatgaaaa tttagttaac tgttaccgta
      541 ctaaaattta tgtcagaaga tgttccatta ccgaaagtca atcagcgata caaagacgat
      601 catggtgcgc ttgtcaccct gaccagtgtt gaggaaaccc gggtggtgtt tatgcaggac
      661 ggctatccgc atccctgcat gcgaccgatg tacaactttc tgggaaaatt caaacccgaa
      721 ccccgggaag aaacagaata aaacgaaatc cctgccggca atcacactac ctgatagcag
      781 cataatccac ttattaattc tcatccccgg agacaagccc agaaaaccac tccgggtgtt
      841 cactgtatgc cgtgttcagc gcatccctgg aaacgtttat ataaaagctg tcctgcaatt
      901 tgcgggtaac aacttctgcc aggcgattcc ggttatccac cagtccggca taacctctct
      961 ctgccagcca ctcattcagt tcatgttcca gagacagatt gataatatca taatcggacc
     1021 ttgccattcc cgtttcctta gcacatgttt tcaggcacgg ggataaagta taaagaatgt
     1081 catactgctg ctgccagagc aatattcccc ctgatggttg tatcagagat ggtatgccgg
     1141 tactttgcgg gttgttcccg tcaggttatc gtgcacctcc acggtcgtgg tcaccaccgg
     1201 cattccggcc tttctcagcc tcagaacatc agccgcaata cgctgactgc caaacctgaa
     1261 caggccgtcc agtgcagtca ccagtaaacc cgcctccagt gcatacttca gccgttcacg
     1321 gggcgctttc acttcccggg caacctgctg atatggcaat gatgtgtttt cattcccaac
     1381 cacgcgacga atacggtgag acagatgata ccggtatgta tccggcacac cagaaaggct
     1441 ggccttcagg ctgtacacgc agccaaaccg tttatcgtta aacaccacat ttttctggct
     1501 gatgccccat tcttcacgca gcgcggcaat caattgcggt gtacgggtaa gcagcaagcg
     1561 aaaaggcagt tcaaaactgg tgacgtaatc cacattcagc agtgcaatac gcaaccgttc
     1621 ttctggcccg gcctctgtct gccggcactc ctccagaaca tcctgccact gccggcgaag
     1681 acggggggat tcgttccgtt ctgtaaaaca gtatttatcc gccagatagt caattcgtgt
     1741 acgcatactg aaaggaattc cgtataaaga ttcagcagac aaaactttat cagccagtaa
     1801 aaattaacgg aagcattgat attcctcccg acaatcaccg gatgattgtt gcaataacct
     1861 gcgacataaa ggactgaaca gccgttttta acgccacgta ttgccctgat gaaccaggcc
     1921 gggccacccg aaaacatctt tccacccggc attgtccgcc agcacttatc accgaccagg
     1981 tctgttgcag taaatccacc agttgcgcac attcttcatc agccatgacg ccgactcgcg
     2041 gatggcaaat actcgtctgg ctgagcagca acagacatcg tatctgtgct ccttcatgtt
     2101 gttcccgcaa ccagcgtaat accaccggcg ctgatgggct gaccgtgtga ttatgctccg
     2161 taatacgttc gtgtacccgt cggtgaaagg aactacaaaa tgtctgaatc tgttgaccat
     2221 agattccttc tgccgaatag agcgtgaagt gaaatgccaa cagatttggc cagtggtcca
     2281 ccgcctgctg aacaaaacgc cggatttccc ccggctctga aattaaggct tcggttattt
     2341 gcacgaaatg ttccttttca acccctgttg aattttgtca agccggtgca gacacagtaa
     2401 agcgcgcatc aacacctgaa aggtacgata caaacacaag ttacaaacaa taagaacgga
     2461 aggacaatta atcagtgagg aataataact aaattaacca aaagaatgtt actgacaata
     2521 ctaccaatac ttaaatagca atatattaat taatgtaaga acagatgccc ggtaggacat
     2581 atacacaata catccacaaa cacactcttt tcattaaatc aatcacctga ttaaaccatg
     2641 aaagtgtgat gtaagtcaaa attactgata ctatcaattg ataatgatta tctctatcat
     2701 tatcgtgaaa ttcgagaatg aaaggagaag tagtcattat aagcctttta taaatcgtca
     2761 tcatcatagt aaagtagaac actttttata tgagttacta tatgaaaata tactggacaa
     2821 gaaaacgtat tcctgaactt tcagcacttc caccttcatt aagaaagaaa aattttactg
     2881 acgcatataa tgcagcatct tctcatatcg aatactggat aggtgctggc gtctcattta
     2941 tctcgatgat gattttatcc agagtatatg actttctttt acccgcccaa gatactttcc
     3001 cgggtgatat catcagaagt ttatgtgttg tgtgtccatc catattaatc tggtttcagt
     3061 tttcggtata tgtcatgagg aaatattaca gacatattct ggtgcgagga aaagaaaccg
     3121 aaaccatttc agaaaggttg atacgtgagg ctgatacacg agaatatgaa ctatggcgtc
     3181 cggtccgtcg ctttttctcc attgttttcc tcattgttct attaggttgc attcattcac
     3241 tgataacaac tataaaataa aagttatggc tgttctgata caggaaaata aacgttcatt
     3301 ctatggttac ctcttcctg
//
LOCUS       DABSYT020000226         3300 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.228, whole genome shotgun sequence.
ACCESSION   DABSYT020000226 DABSYT020000000
VERSION     DABSYT020000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.228"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..659)
                     /locus_tag="ID725_RS24955"
                     /old_locus_tag="pgaptmp_004991"
     CDS             complement(<1..659)
                     /locus_tag="ID725_RS24955"
                     /old_locus_tag="pgaptmp_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGV7853390.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGGSPKMRDIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTK
                     DTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMV
                     KT"
     gene            complement(692..988)
                     /locus_tag="ID725_RS24960"
                     /old_locus_tag="pgaptmp_004992"
     CDS             complement(692..988)
                     /locus_tag="ID725_RS24960"
                     /old_locus_tag="pgaptmp_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGV7853391.1"
                     /translation="MEQTNYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITNKKRPAATERSEQIQMEF"
     gene            complement(1127..1354)
                     /locus_tag="ID725_RS24965"
                     /old_locus_tag="pgaptmp_004993"
     CDS             complement(1127..1354)
                     /locus_tag="ID725_RS24965"
                     /old_locus_tag="pgaptmp_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7853392.1"
                     /translation="MQNLDEPIKGVGIPEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGKYQASEMLEISKKNLLAA"
     gene            1433..2140
                     /locus_tag="ID725_RS24970"
                     /old_locus_tag="pgaptmp_004994"
     CDS             1433..2140
                     /locus_tag="ID725_RS24970"
                     /old_locus_tag="pgaptmp_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGV7853393.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTLSFVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            2201..2542
                     /locus_tag="ID725_RS24975"
                     /old_locus_tag="pgaptmp_004995"
     CDS             2201..2542
                     /locus_tag="ID725_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HGV7853394.1"
                     /translation="MAFNDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRADPDCCFFG"
     gene            2610..3071
                     /locus_tag="ID725_RS24980"
                     /old_locus_tag="pgaptmp_004996"
     CDS             2610..3071
                     /locus_tag="ID725_RS24980"
                     /old_locus_tag="pgaptmp_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853395.1"
                     /translation="MRILGVRAAPKVTSFVVYCTNESALKCVDVIKIPSTLDTPEKLK
                     YVRNNILDILNLYNVELAAIRVTESNSDNLSIDRLYIEAVIQEAFSSSDVRKYYTIRK
                     SGMKSSLNLTEIEYKEILKSHRNINGIDNSGFTAETNEAVLAALSAEVRGC"
     gene            3065..>3300
                     /locus_tag="ID725_RS24985"
                     /old_locus_tag="pgaptmp_004997"
     CDS             3065..>3300
                     /locus_tag="ID725_RS24985"
                     /old_locus_tag="pgaptmp_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HGV7853396.1"
                     /translation="MLTPYKRADVEFEWISDLEEQGCFSKVYLAHDRHLAHDLVIKEI
                     EKKENTNHDDYFNEARLLYKHAHPNIVQVQYAAQ"
BASE COUNT          925 a          652 c          748 g          975 t
ORIGIN      
        1 gtcttcacca tgtcaaacat ccactctgcg gcggtcaggt cttctgctgt tccccacttg
       61 ctgccgctct gaattgcagc atccggttta accacagaaa gatcgttttc tggctggtca
      121 gaggattcgc cagaattctc ggacgaataa tcttttcttt tttcttttgt aatagtgtct
      181 tttgtgtccc cctgttttga gggatagcaa tcccctaatt tgagggatgt tttatccctc
      241 gttttagggg attttccctc gttttgaggg atgtccctca ttttagggga acctccctcg
      301 ttttgaggga tgcaccattc tgagatgttt ttatttggtc caaacatgcc gccttgctgc
      361 ttgataatat tcattctgac gagttctaac ttggcttcat tgcaccgttt gacaggtaac
      421 tttgtaatct cgctaagttg agaatcggtg attctgtcca ttggtttatt ccacccatag
      481 gttttacgca gaatggcaag cagcacttta aactgtcgct tggtcagatc tgcgcctgaa
      541 taagcctcaa tcagcatatt tgatagtctg gcgtaaccat catcgagatc tgccacatta
      601 cgctcctgtt cggcaaagtt acctctgccg aagttgagta tttttgctgt atttgtcata
      661 atgactcctg tagattgatc cagtaatgac ctcagaactc catctggatt tgttcagaac
      721 gctcggttgc cgccgggcgt tttttattgg tgatttcatc aagcgcatac ttaaaagctc
      781 tgctaatcgg actgatgtct gatgccatgc caaaagcaca caagaccgaa gctataaacc
      841 tccagtctgt tctgcttatc ttcgattcat gacagccaat catctttgcc agaccgcgct
      901 gagtaagcgt tgacaggttg ataagtaaat ctgtttctgc gcgatcaacg tcgcgctgtg
      961 atagtttgct gtaatttgtt tgttccattt cttaagattt ccaatagtga atagttagtt
     1021 gaaaggtatg cgtggaaacg catatggcct tagttggtca gatatattgg gactcgcttt
     1081 gtcagcgacg taggacgaat gtccattgtg aaaatagcgg tgttacttat gcagccagaa
     1141 ggttcttttt gcttatttca agcatttcgc ttgcttgata tttgccacca gaaatctctt
     1201 cgatttttga tgcgtattta gttttcccaa aaaactcagt cttagggagg aagccgtttt
     1261 tgagccactt atagacagcc ctttcgctaa ctccacaagc cttcgcaact tcagggatgc
     1321 cgacaccttt aatcggctca tcaagatttt gcataggaat atcctttttc gtactttcag
     1381 tacgtattat ggttgaactg aaagtttttg caagtgcttt agtatcgtac tcatggttca
     1441 gaatgaaaaa gtgcgcaaag aattcgccca gcggctagcg caagcctgta aagaagctgg
     1501 tcttgatgaa catggtaggg gaatggctat agcccgtgcc ctttctcttt cgtccaaagg
     1561 cgttagcaaa tggtttaatg ctgagtcttt accgcgtcag gaaaaaatga atgcgcttgc
     1621 gaaatttcta aacgttgatg ttgtttggct tcagcacggc acttcgttaa atggagcgaa
     1681 tgatgaagat actctttcat ttgttggcaa attaaaaaaa gggttagtgc gcgtggttgg
     1741 tgaggcaatt cttggtgttg atggtgccat cgagatgacc gaagagcgcg atgggtggct
     1801 caaaatttat agcgatgatc cagatgcctt tggtcttcgt gtgaaaggag acagcatgtg
     1861 gcccagaata aaatcaggag aatatgtact cattgagcct aacaccaaag tattcccggg
     1921 tgatgaggtg tttgtcagaa ccgttgaagg acacaacatg attaaggttc ttggctatga
     1981 cagagatgga gaataccaat ttacaagcat taaccaggat cacaggccta taacgttgcc
     2041 ttatcatcaa gtagcaaagg tggagtatgt agctggtatt ctgaagcaat ctcgccatct
     2101 ggatgacatc gaggcaaggg agtggctgaa aagttcgtga cttcatcgtc acatagctgg
     2161 taaccagtgg cctgaagaga cgtttgggta aggaggatag atggcgttca atgaccttga
     2221 atatcaagca gtaaaaaaag aagttcacca attcattgaa agcataaggc cgcctgaaca
     2281 tatccgcaat gaactggata ttgtttatag catcaatgac caaacgatag atatcggcga
     2341 acagcgcccc gtgtggcagg gcaatccagg tgaaacaaac atcctgccat cagcaagaat
     2401 caagtacata cgttctctgg atagatggaa aatctattgg atgcggaagg atatgaaatg
     2461 gcatcagtac agtactgaac tttcgctgac tgatgcgctt gagcttgtgc gtgctgaccc
     2521 ggattgctgc ttcttcggat gagtgaagag acgtttggat gatggatggt cgcagagatg
     2581 cggcctgatt ctaaaatagg atataaaaaa tgagaatact aggtgttaga gcggcgccca
     2641 aagttacatc ttttgttgta tattgcacta atgagtctgc actcaaatgt gttgatgtta
     2701 ttaaaatacc ttcgacctta gacacaccag aaaaattaaa gtatgtgaga aataacatcc
     2761 tcgacattct taatttatat aatgttgaat tagctgccat acgcgttact gaatcaaact
     2821 ctgataatct tagcattgac cgcctttata tagaagctgt tattcaagaa gcattttcaa
     2881 gcagtgatgt aagaaaatat tacactatta gaaaatctgg catgaaatca tcattgaacc
     2941 taacagagat cgagtataaa gaaatattga agtcacaccg caatataaat ggaatcgata
     3001 attctggttt tacagctgaa acaaatgaag ctgttttggc tgcactatct gcggaggtaa
     3061 ggggatgcta actccataca aaagagctga tgtagaattc gaatggatta gtgatctaga
     3121 agaacagggt tgtttttcaa aagtatatct ggctcatgac agacacctag ctcatgactt
     3181 ggtgattaaa gaaatagaaa aaaaagaaaa cactaaccac gacgactact ttaatgaagc
     3241 aaggcttctc tataaacatg cacatccaaa tattgtgcaa gttcagtatg ctgctcaatg
//
LOCUS       DABSYT020000227         3293 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.229, whole genome shotgun sequence.
ACCESSION   DABSYT020000227 DABSYT020000000
VERSION     DABSYT020000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.229"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(218..385)
                     /locus_tag="ID725_RS24990"
                     /old_locus_tag="pgaptmp_004998"
     CDS             complement(218..385)
                     /locus_tag="ID725_RS24990"
                     /old_locus_tag="pgaptmp_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGV7853397.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(382..813)
                     /locus_tag="ID725_RS24995"
                     /old_locus_tag="pgaptmp_004999"
     CDS             complement(382..813)
                     /locus_tag="ID725_RS24995"
                     /old_locus_tag="pgaptmp_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HGV7853398.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQVVINAAYLVACADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(983..1059)
                     /locus_tag="ID725_RS25000"
                     /old_locus_tag="pgaptmp_005000"
     tRNA            complement(983..1059)
                     /locus_tag="ID725_RS25000"
                     /old_locus_tag="pgaptmp_005000"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1023..1025),aa:Arg,seq:tct)
     gene            complement(1160..1235)
                     /locus_tag="ID725_RS25005"
                     /old_locus_tag="pgaptmp_005001"
     tRNA            complement(1160..1235)
                     /locus_tag="ID725_RS25005"
                     /old_locus_tag="pgaptmp_005001"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1200..1202),aa:Ile,seq:cat)
     gene            complement(1264..1977)
                     /locus_tag="ID725_RS25010"
                     /old_locus_tag="pgaptmp_005002"
     CDS             complement(1264..1977)
                     /locus_tag="ID725_RS25010"
                     /old_locus_tag="pgaptmp_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7853399.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     VTDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAVAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(2113..2310)
                     /locus_tag="ID725_RS25015"
                     /old_locus_tag="pgaptmp_005003"
     CDS             complement(2113..2310)
                     /locus_tag="ID725_RS25015"
                     /old_locus_tag="pgaptmp_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGV7853400.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRIQS"
     gene            complement(2535..3089)
                     /locus_tag="ID725_RS25020"
                     /old_locus_tag="pgaptmp_005004"
     CDS             complement(2535..3089)
                     /locus_tag="ID725_RS25020"
                     /old_locus_tag="pgaptmp_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGV7853401.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(3152..>3293)
                     /locus_tag="ID725_RS25025"
                     /old_locus_tag="pgaptmp_005005"
     CDS             complement(3152..>3293)
                     /locus_tag="ID725_RS25025"
                     /old_locus_tag="pgaptmp_005005"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGV7853402.1"
                     /translation="IKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIV
                     RG"
BASE COUNT          873 a          910 c          675 g          835 t
ORIGIN      
        1 cgcgccatgc gcagccggaa ataaagggat aacgatgata gtttgagaaa aacagaaaca
       61 acacttttgt ggcaaagcat ggtgccgggt gcctcccggt gaattcagta tcagcacctg
      121 aatccgcgat tatcccatat acctggttgc tgatcgcccc tccgcacagg gggattcacc
      181 atgcagaagt gtttttaata aacagcaaac aaaaaaatca agcattatgc aggctgtttc
      241 tttttatcac cggccacagc aataccacaa tgccgcagac cagcacccca tccgccagca
      301 ccgacatgat tctgctggtg aaatccacca tcaccaccag aaacagcagg agtgcagcca
      361 cagccaggcg cagttttacc gtcacaggtg attctccaga cgaagaccca gaacaccggc
      421 aatctcttcc agcaccttgc gctcttccgg ctcaatttcg ccgtctgcct ccgcaatggc
      481 caccgccaca tccagcacat cttccgcttc acgcgtatcg tgtttcacat cctcaatctc
      541 acgtaacgcc gcacgacgac cagttttaaa gttcgtatcc agctgaccga taatggttgc
      601 gctaatcgca ttaatttctg acgtaaacgc ggacagcgca ggctgattac gcagtacctg
      661 ttcgatcttc gctttctcgg atgcctcaca ttcaccatct gcacaggcca ccaggtatgc
      721 ggcgttaatc accacctgtg ccagatcgcg tttttcaaac tttttaattt ccgttgccgc
      781 tctgcgggtt ttctttttga agattccaaa catcgtgacg ttcctttggg tgggtgagcc
      841 aacgcccggg agcgatctgc ccacagagaa agtcacactg accactccgt aagctcaccc
      901 ccgaaaggct ctgtggttgg tatgcgccgg gcgtggcgca gatacaaaaa aggcccgccg
      961 aagcgagcct ggaaaataag tgtggcgcgt tgtagtggag tcgaaccact gaccgattgc
     1021 ttagaaggca attgctctgt ccggctgagc taacaacgca gaataccgat aatggaccgc
     1081 catcggggac ccggccccgc accaacaacc ctgttatggt gtcgtctgct cttcctgata
     1141 agctaatggc ggtttgtgat ggtggccctt gctggatttg aaccagcgac ctggcgatta
     1201 tgagtcgctc gctctcacca ctgagctaaa gggccgagcc aaaaaataat aatcagatga
     1261 aaatcaataa tcaagccctt gcctggatac atatctgtct ggcgggaagc cataatagcg
     1321 gtgaaataca gaaataaagt aggaccggct tgaataacca catttttctg ctacagcctg
     1381 tccatatcca taccgggaac ataacatatt tacagcaaca cgcatccgct cttccagcaa
     1441 caagcgactg aacatgcccc cttcattttt cagttttgtc tttaacaaac tctcactcat
     1501 atgcaactgt agagcaatcg caccaagcgt ccagcttgct gatatatctg tctgaattat
     1561 cgccctgact ttggcactta tgctggataa acatccactt aaaaacaatg acatccgttc
     1621 atctgtttca aacagagaca ggcaggccat cataagaaac atatccgtga cctctccgga
     1681 aaatccctgg ctggtaatta aagccgcagc caacgcagga ttgttgggtt ccagcaacag
     1741 gtaaagcgga atgtcagtca gacgagtcct cgtcagctta tgctgacttt ccagatattg
     1801 acttacgacg gattcgctta tatcgacaat tttaactttg ccataatgca taaggaaaag
     1861 ctccctgatg catttggtgg ccagaacgac tgagcctggc ttaagtgaca acgtatcctt
     1921 ttcaagaaaa atattaattg gggagcaaac catgataact gaacagacaa cagccattat
     1981 aattttactt tcattagcaa ttggttagct caattatagc cccaaaaggt aaattatcat
     2041 caacacataa gcaaaggact gacaggtgcc gcccccccca ccagccgccc atttaccaca
     2101 aataaaaagc cttcaggact gaatgcgtct gtaacaaccg cactgatagt ctgccagacc
     2161 cgccataacc agctgggtca gtattaactg gcagcgttcg cgtgaaaggt aagtattctg
     2221 cgcaatctcc ccgactgtcg ccggttcggt aacgcttaat tcattaaaca ccactctggc
     2281 ggtttctgtc atatcctgct gttttagcat gtctttttcc cttttccggt taacgtgaca
     2341 taccaataac tcttgtcgaa aaagccagca agttgaaaga ccggtattag caaccaccag
     2401 cgcgtttaac gccccgtgcc gtttttcagt cataaaaaaa cccgcaaaaa gcgggctctt
     2461 tcaaatgtcc atgtctgcta ttcgcctcgc ggtacagctt tgcgaagctt accggaattg
     2521 aagcagtttt tacgtcaaaa agcaataact tttttctcta taccaaaagc cataaccatt
     2581 ggtttgtaca aaataaattc tgccaccttt agccaatgct caatgcgtct ttcacaggtt
     2641 cttaaactcc attccggatg tgcatcattc agcaattcag ccattttgcg cttagtcatc
     2701 ccccgcccca cataacgctg actcagaaca ttgagcagcc cgggataacc tgccagtact
     2761 tcaccaataa ccctatcgat tattaacgcc tctgaatcgg tacaatgtgc cagccagctt
     2821 ttttgattgc cgttgatcat atcccgcaaa aaagcctcaa gttcaggttt gtccagaccc
     2881 gcttttttca tcctccggag cgcctcgtta attgccgttt ttgtcagctt tttagaggtc
     2941 agtaattggt tgaacatatt tcccgtctta ccgtcgccaa tatacgacca acgcccccac
     3001 atacgcagtt tcccctggat ccagacactt tccagcgttt tcaggcgtaa atgctcaccg
     3061 cttttgcctg taatttccgg gtatatcata tttatgctca ctcactttca attttgtaaa
     3121 tcttcacgcc cagccgccca ccagaaacga gctagccgcg cacaatattg atttcatcaa
     3181 actgctcgtc gtctatgaga agtccggcat gcgtcagcgc atccagtggt gctttcagga
     3241 tattgtccag gtcacgacgg cgcttatccg gtggctctgc aataatcttt atc
//
LOCUS       DABSYT020000228         3277 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.230, whole genome shotgun sequence.
ACCESSION   DABSYT020000228 DABSYT020000000
VERSION     DABSYT020000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.230"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            13..1338
                     /gene="nleA"
                     /locus_tag="ID725_RS25030"
                     /old_locus_tag="pgaptmp_005006"
     CDS             13..1338
                     /gene="nleA"
                     /locus_tag="ID725_RS25030"
                     /old_locus_tag="pgaptmp_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HGV7853403.1"
                     /translation="MNIQPTIQSGITSQNNQHHQTEQIPSTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNV
                     TQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDIIQETRV"
     gene            complement(1678..2252)
                     /locus_tag="ID725_RS25035"
                     /old_locus_tag="pgaptmp_005007"
                     /pseudo
     CDS             complement(1678..2252)
                     /locus_tag="ID725_RS25035"
                     /old_locus_tag="pgaptmp_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            complement(2365..2634)
                     /locus_tag="ID725_RS25040"
                     /old_locus_tag="pgaptmp_005008"
     CDS             complement(2365..2634)
                     /locus_tag="ID725_RS25040"
                     /old_locus_tag="pgaptmp_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853404.1"
                     /translation="MNILKKLMQRLCGHGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(2636..>3277)
                     /locus_tag="ID725_RS25045"
                     /old_locus_tag="pgaptmp_005009"
     CDS             complement(2636..>3277)
                     /locus_tag="ID725_RS25045"
                     /old_locus_tag="pgaptmp_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853405.1"
                     /translation="SAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPK
                     GDKGERGDTGPAGATGERGPAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTG
                     AAGPAGPQGPKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPASIIETNSHGWFPGT
                     DGALITGLTFLAPKDTTRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          906 a          835 c          650 g          886 t
ORIGIN      
        1 taaggattta ttatgaacat tcaaccgacc atacaatctg gaatcacctc acaaaacaat
       61 caacatcatc aaacagaaca aataccctct acacaaatac cgcaatccga attacctcta
      121 ggatgccaag ctggatttgt tgttaatatt ccagatgata tacagcaaca tgcaccggaa
      181 tgcggtgaaa caacagctct actgagcttg ataaaagata aaggtctgct ctcagggcta
      241 gacgaatata tagctcctca ccttgaagaa ggatccatag gaaaaaaaac attggatatg
      301 tttggtttat tcaatgttac ccaaatggca ttagagatac ctagttccgt ttcaggcatc
      361 tctggtaaat atggtgtcca gctaaacatt gtaaaaccag atattcatcc tacatcaggt
      421 aattattttt tacagatatt ccctctgcat gatgaaatag gttttaattt taaagacctt
      481 cctggcccgt taaaaaatgc attaagcaac agtaatatat caaccactgc agtgtcgact
      541 attgcatcga ctggaacatc agccactact tcgacggtaa ccaccgagcc aaaagaccca
      601 ataccatggt ttggattaac agctcaagtg gttcgtaatc atggtgtaga acttcctata
      661 gtcaaaactg aaaatggatg gaagcttgtt ggagaaacac cacttactcc tgatgggccg
      721 aaagcaaatt acacggagga gtgggttatc agaccgggag aagcagattt taaatatggt
      781 gcatctccat tacaggcaac tctagggctg gagtttggcg cacatttcaa gtgggattta
      841 gataacccta atactaaata tgccgttctt accaatgctg ccgcaaatgc gcttggtgct
      901 ttagggggat ttgcagtatc cagatttgct agtacagatc caatgttaag tcctcatatc
      961 ggtgcaatgg ttgggcaagc agcagggcat gctatacagt ataatacccc tggattaaag
     1021 ccagacacta ttttatggtg ggctggtgcg acactggggg ctgccgattt aaacaaggcc
     1081 gagtttgaag tagctagatt cactgactat cctcgtatat ggtggcacgc aagagaagga
     1141 gctattttcc ccaataaagc agatattgaa catgccacag gtgctgatat acgcgcaatg
     1201 gaagaaggta tccctgttgg acagcggcat ccaaatccag aggatgtggt aatcgatatc
     1261 gaaagcaatg gcttaccaca tcataatcca tcaaatcatg ttgatatctt tgatataatc
     1321 caagaaacaa gagtctaagc tttaacttgt ttattttaaa ataatagctg aaatggatgg
     1381 cagctattat taataaggat taatatattt tttctaaaat accccacaaa acaaacacat
     1441 cataaaaatt ctaaatacac acacctaatc tcaaaaaaaa caaacttact atttgacttg
     1501 gattatgttt ttaatccaat attttccgac aacatctcca cagaaaaatg catttacata
     1561 aagacgcctt caagtcatct gaaacctgcc aaccacagac accttcttat attctggatg
     1621 tctgcggcgt ttttttacaa attactgaca gccaatactc tctcagagaa aaatatttta
     1681 actatctttt ataatgaagt ttcccttgat aggatcataa acacatttat caggacttat
     1741 aatcattgat gccgttattg gttctcgggt cagtggatga tatgagcctt cactagctaa
     1801 acgagaaaat gcatcaaaat caaataagca gcatactgcc gaactatctg aatttttgac
     1861 aaacactcct tcttcgggtc tctctagtgt aattggacac tgaatagact ctggcgggca
     1921 ctggaaattt cctgaactaa caggaaactt acattgagat attttgtcct gaatactctc
     1981 ttgcgattgt gcttcacctg agtccgaaag catactgagc atcctatcgc gaagagcttc
     2041 agggccgtta ttaaacccat aaaggaataa ctcagtaagc aatcccccac tactgccgct
     2101 ggatagaaaa cgcccatcgg tttcattaaa tacaatgctg acagtctcat tacctaatgt
     2161 aaactcccct cgaccgctag atgctacttg agctcgaagg acattcaatc cattaaaaat
     2221 gagcttgatc taatctccga atttaacggc attataaaac tccctatttt aatttaaact
     2281 ccagacttaa atagctttaa caaacatctg ccttacatga caacaaaaac cggagccgga
     2341 ctccggtttt gtgaagctgt cgggttactt catcccgcca atattttccc acgtcccgtc
     2401 agcacgcagg atttgcagcg gtcttaccac gcactgtatc tgctttttat ccgcatccag
     2461 tatcaccacc tgcgtgatta ccctgtcctg ctccggaata ataccattct catcggactc
     2521 caggatgtct gccggcccca gacgcagttg tgctgtaagt aactccccgt cttcacggtc
     2581 atcatgcttt ccgtgcccgc acagacgctg cataagtttt tttagtatgt tcatgtcatt
     2641 ctcctgttct gcctgtatca ctgcccactt catcaagccc cttaacatcc tgccacggcc
     2701 cgtcaccaaa cctgacctgc aaatgctgaa aaaaaccctg aacccgtgtg gtatctttgg
     2761 gggcaagaaa ggtcagtccg gtgatgagcg caccatctgt acccgggaac cagccatggc
     2821 tgtttgtctc aataatgctc gccggcccca gacgaaaacg gatttgtgtc tcccccgggt
     2881 cgcccttcgg tccctgaggt ccggttgccc ccaccgggcc agccgcacct gtttctcctt
     2941 tcggtccctg tgggcctgcc gggcctgctg ccccggtgtc tccctttgga ccctgtggac
     3001 ctgcatttcc cgtcagaccg gtctctcccc gctctcccct gtcacctttc ggcccctgcg
     3061 ggcctgccgg accagcatca cctgccggtc cccgttcgcc ggttgcccct gccgggccgg
     3121 tgtcgccacg ctctccttta tctcccttcg gcccctgagg acccgcggga cccggttccc
     3181 cctttggccc gggaggtccc accacggtgg ggattcggtt tacggcttct tccgccgcta
     3241 tcctgctttg ttccgctgac tgtgcgcttt ctgctga
//
LOCUS       DABSYT020000229         3259 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.231, whole genome shotgun sequence.
ACCESSION   DABSYT020000229 DABSYT020000000
VERSION     DABSYT020000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.231"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            674..2821
                     /locus_tag="ID725_RS25050"
                     /old_locus_tag="pgaptmp_005010"
     CDS             674..2821
                     /locus_tag="ID725_RS25050"
                     /old_locus_tag="pgaptmp_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGV7853406.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
     gene            3130..3259
                     /locus_tag="ID725_RS25055"
                     /old_locus_tag="pgaptmp_005011"
                     /pseudo
     CDS             3130..3259
                     /locus_tag="ID725_RS25055"
                     /old_locus_tag="pgaptmp_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
BASE COUNT          958 a          715 c          694 g          892 t
ORIGIN      
        1 tatattctga tatactcctt ttgctagaca taacctttca cctgcttgca aagcttctgt
       61 gttctgacat tgccaaattg ttgcaattct gtatccagcc ttctttcagt catagcttcg
      121 ggccgcgata agactcactg atctgaccct gattcctctt gcagacttta tagaccaatt
      181 aaaatgcagt ttctgcaggt caacgtctga ccatcattgt catcactctg gccattagag
      241 taaccttctg cattcatcct tttgtaaaaa gtttatatta gtatcagcaa ttaaccggac
      301 ctgatactga tatgagtctt accgcatata cggtcaattt cagcaattaa ttacattatc
      361 cacgccaaag tatttgtcat cacaatgatg gtaccttctt tcagacacca ttttttcaac
      421 tccgttttcc acggaccgca ctcttatgtc aagagtgcgg tccgtggata caaccagaga
      481 ccgactgaca cgagtcagag gaaacgacgg atatgttcag tcgtaaaata tctatcaaaa
      541 aacatgatta aggtcaaaaa tgtttgatat ttacaattta tgaagatgac aataattata
      601 gatatatgag aacataaatg aaaataatta tcattacagc aatcatttgt actttgtatt
      661 aatgagggat gaaatgttat ataatatacc ttgtcgaatt tatatccttt ccactctgtc
      721 attatgcatt tctgggatag tttctactgc aaccgcaact tcttcagaaa caaaaatcag
      781 caacgaagag acgctcgtcg tgaccacgaa tcgttcggca agcaaccttt gggaaagccc
      841 ggcgactata caggttattg accaacaaac attgcagaac tccaccaatg cctccatagc
      901 cgataatttg caggacatcc ccggagtaga gataacagac aactccttgg caggccgtaa
      961 acaaatccgc attcgtggcg aagcatcctc ccgtgtttta attctcattg atggtcagga
     1021 ggtaacttat cagcgcgccg gagataatta tggtgtggga ctgttgatag atgagtctgc
     1081 gctggagcgt gttgaggtag tgaaaggtcc atattccgta ctgtacggtt cacaggcaat
     1141 tggcggtatt gttaacttca tcaccaaaaa gggaggtgac aaacttgcat ctggagttgt
     1201 gaaagctgtt tataattccg caacagcagg ctgggaagaa tcaatcgcgg tccaggggag
     1261 catcggtgga tttgattatc gcatcaacgg tagttattct gatcagggca atcgtgatac
     1321 gccggatgga cgtctgccga ataccaacta tcgtaacaat agtcagggtg tatggttggg
     1381 ttataactcc ggaaaccatc gttttggcct ctcgcttgat cgctacagac tcgcgacaca
     1441 aacttactat gaggatccag acggaagtta tgaggcattt agtgtcaaaa tacctaaact
     1501 tgaacgagag aaagttgggg tattctatga cacagacgtg gacggtgact atctaaaaaa
     1561 aattcatttc gacgcgtatg agcagaccat ccagcgccaa tttgccaacg aagtaaaaac
     1621 gacacagcct gttcccagtc cgatgattca ggctctgacc gttcataaca agactgacac
     1681 ccatgataag caatacactc aggcggtcac attgcagagt cacttttcgc tgcctgctaa
     1741 taatgaactt gttaccggtg cacagtacaa acaagacagg gtcagccaaa ggtccggtgg
     1801 catgacctca agcaaatctc tgaccggctt cattaataag gaaacacgaa ctcgctccta
     1861 ttatgagtca gagcaaagta cagtctcact attcgcacaa aatgactggc aattcgccga
     1921 tcactggaca tggacaatgg gagttcgcca atactggctt tcttcaaagt tgacgcgtgg
     1981 tgacggagta tcatataccg caggcattat aagcgatacc tctcttgcca gagagtctgc
     2041 gagtgatcac gaaatggtaa catctacaag cctgcgctat tcaggtttcg ataacctgga
     2101 gttacgcgct gcgttcgcgc aaggctacgt atttcccaca ctctcccagc tttttatgca
     2161 gacatctgcg ggcggcagtg tcacatacgg aaatcctgat cttaaggctg aacactccaa
     2221 taactttgaa ttaggtgcac gatataatgg taatcagtgg ctgattgaca gcgcagttta
     2281 ctactcagaa gctaaagatt atattgcaag tctgatctgt gatggcagta tagtttgcaa
     2341 tggtaacacc aactcctccc gtagtagcta ctattattat gacaatattg atcgggcaaa
     2401 aacatgggga ctggaaataa gcgcggaata taatggctgg gttttctcgc catatatcag
     2461 tggcaattta attcgtcggc aatatgaaac ttcaacatta aaaacaacta atacaggtga
     2521 accagcgata aacggacgta tagggctgaa acatactctt gtgatgggtc aggccaacat
     2581 aatctctgat gtttttattc gtgctgcctc tagtgcaaaa gatgacagta acggtaccga
     2641 aacaaatgtt ccgggctggg ccactctcaa ctttgcagta aatacagaat tcggtaacga
     2701 ggatcagtac cggattaacc tggcactcaa taacctgaca gacaaacgct accgtacagc
     2761 acatgaaact attcctgcag caggttttaa tgcagctata ggttttgtat ggaatttctg
     2821 atgacaaaaa caatcgttat ggaattcttt gtcctgatct gcccgctttg cgggcaggct
     2881 aacgaacgta ttgtgtttgt tgaaggtttt tcgacgttgt tttccatctg tgggtgaata
     2941 tttattcttc ccgccagcat gactctatac tcattgctac gacaatgact tataaagctg
     3001 gttactgaac ctataccttt gtaattcaaa agtgatatca gttcacaacg caatactatt
     3061 tttagattat ttactctcac ccagatactg aatcaatgga taataccttt tgatttatcc
     3121 cgacgcggaa gaaggtctca gtccggatta tgggggaaaa tttctcaatg gtccggtatc
     3181 aacaaccagg gccgggagga ttacaggaaa cgagattttg ccccggacag ccccgaaata
     3241 aaattcagtg atcgcaggg
//
LOCUS       DABSYT020000230         3164 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.232, whole genome shotgun sequence.
ACCESSION   DABSYT020000230 DABSYT020000000
VERSION     DABSYT020000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.232"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            111..689
                     /gene="espA"
                     /locus_tag="ID725_RS25060"
                     /old_locus_tag="pgaptmp_005012"
     CDS             111..689
                     /gene="espA"
                     /locus_tag="ID725_RS25060"
                     /old_locus_tag="pgaptmp_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HGV7853407.1"
                     /translation="MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQA
                     AILMFSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAK
                     LPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            702..1826
                     /gene="espD"
                     /locus_tag="ID725_RS25065"
                     /old_locus_tag="pgaptmp_005013"
     CDS             702..1826
                     /gene="espD"
                     /locus_tag="ID725_RS25065"
                     /old_locus_tag="pgaptmp_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HGV7853408.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            1847..2785
                     /gene="espB"
                     /locus_tag="ID725_RS25070"
                     /old_locus_tag="pgaptmp_005014"
     CDS             1847..2785
                     /gene="espB"
                     /locus_tag="ID725_RS25070"
                     /old_locus_tag="pgaptmp_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HGV7853409.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            2792..>3164
                     /gene="cesD2"
                     /locus_tag="ID725_RS25075"
                     /old_locus_tag="pgaptmp_005015"
     CDS             2792..>3164
                     /gene="cesD2"
                     /locus_tag="ID725_RS25075"
                     /old_locus_tag="pgaptmp_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HGV7853410.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETE"
BASE COUNT          924 a          613 c          779 g          848 t
ORIGIN      
        1 tgcattatta atgattggta aagttatcga ctataaggag gaaattattt gatattaatt
       61 actcaataat ttttttgttt tcctgagaaa aattatcaag aggtatatag atggatacat
      121 caaatgcaac atccgttgtt aatgtgagtg cgagttcttc gacatcgacg atctatgact
      181 taggtaatat gtcgaaggat gaggtggtta agctatttga ggaactcggt gtttttcagg
      241 ctgcgattct catgttttct tatatgtatc aggcacaaag taatctgtcg attgcaaagt
      301 ttgctgatat gaatgaggca tctaaagcgt caaccacggc acaaaagatg gctaatcttg
      361 tggatgccaa aattgctgat gttcagagta gcactgataa gaatgcgaaa gccaaacttc
      421 ctcaagacgt gattgactat ataaacgatc cacgtaatga cataagtgta actggtattc
      481 gtgatcttag tggtgattta agcgctggtg atctgcaaac agtgaaggcg gctatttcag
      541 ctaaagcgaa taacctgaca acggtagtga ataatagcca gctcgaaatt cagcaaatgt
      601 cgaatacatt aaatctctta acgagtgcac gttctgatgt gcaatctcta caatatagaa
      661 ctatttcagc aatatccctt ggtaaataac cggagataac tatgcttaac gtaaataacg
      721 ataccctgtc tgtaacgtct ggggttaata ccgcctcggg tacttctggt attactcaat
      781 ctgaaacggg tttatcgctg gatttacaac tggttaaatc catgaactcg tcagcaggct
      841 ggacagaaag tagcccttta ccgacgccgc cggcaggtca ctcattagtg acgccctctg
      901 ctgctgagga tgtccttagt aaattgtttg gtggtattag tggtgaggtt acaagtcgca
      961 ctgaggaggc agagccacag cgcacaagct atccctatct ctctcaggtg aataccgttg
     1021 accctcagca aatgatgatg atggtcactc tgttatccct ggatacttcc gcgcagaaag
     1081 tctcgagtct gaaaaactct aacgagattt atatggatgg gcaaactaaa gcgctggaga
     1141 ataaaacgca ggagtataaa aaacagctcg aagaacaaca gaaagccgaa gagaaatcac
     1201 aaaaaagtaa aattgttggc caggtctttg gttggttggg cgtcgcatta acagccgttg
     1261 ccgctgtttt taacccagca ctctgggctg ttgttgccat tggtgcaaca gcaatggcac
     1321 tgcaaacggc agtcgatgta atgggggaaa atgcccctca gggattaaag actgcagcac
     1381 aggtctttgg cggaatatct atggccgcaa gcattctgac agccggcgtt ggcggggtgt
     1441 cttcactgtt atctaaattt ggtaatgttg ctaacaaaat tggctcaagc gttgtaaaag
     1501 tcgttgagaa ggcggcagaa gcgctggtta aaaacgtttt tgcaaaaatt tcgacagtgg
     1561 ctgagggcgt tacgaacggt attcgttctg ccgggacaac tgcgttgaat aatgaggctg
     1621 cgcaactcca aatgttgtct cagttagctg ctttcgcggt gcaaaactta actcgacaga
     1681 gtgaaagctt aggtgagagt gcgaagctcg agctggataa agcggcaagc gagttacaaa
     1741 atcaggcgag ctatttacaa agtgtttctc aactgatgtc cgattcagca cgggtaaata
     1801 gtcgtattgt tagtggccga atttaattat taaagagaat tttattatga atactattga
     1861 taatactcaa gtaacgatgg ttaattccgc ttcggagagt acgaccggcg cttccagtgc
     1921 agttgccgca tctgctttat caattgattc atctctgctt actgatggta aggttgatat
     1981 ttgtaagctg atgctggaaa ttcaaaaact cctcggcaag atggtgactc tattgcagga
     2041 ttaccaacaa aaacaattgg cgcaaagcta tcagattcag caggccgttt ttgagagcca
     2101 gaataaagct attgaggaaa aaaaagccgc ggcaaccgct gctttggttg gcgggattat
     2161 ttcatcagca ttggggatct taggttcttt tgcagcaatg aacaacgcgg ctaaaggggc
     2221 tggtgagatt gctgaaaaag caagctctgc atcttcaaag gctgctggtg cggcttctga
     2281 ggttgcaaat aaagctctgg tcaaggctac ggaaagtgtt gctgatgtcg cagaggaggc
     2341 atccagtgcg atgcagaaag cgatggccac aacaacgaaa gcagccagcc gtgcatctgg
     2401 cgttgcagat gatgttgcga aagcctctga ctttgctgaa gatcttgcag acgccgccga
     2461 gaagacaagc agaatcaata agttgttgaa ttccgtagat aaactgacca ataccacagc
     2521 atttgttgcc gtgaccagtc ttgctgaagg tacgaaaacg ttgccaacaa caatatctga
     2581 gtccgtcaaa tcgactcatg aggttaatga acaacgtgcg aagtcgctgg aaaacttcca
     2641 gcaggggaat ctggagctgt ataaacaaga cgttcgcaga acgcaggatg atatcacgac
     2701 tcgtctgcgt gatataacgt ccgctgtccg cgatctcctt gaggtccaga atcgtatggg
     2761 gcaatcgggt cgcttagctg ggtaattgat catggtcgat acgtttaatg atgaagtgtt
     2821 taattactat cttgaacaaa aagggtacac aatacagaag gagtttcttt gtggcagtgc
     2881 cttttttatc ggatggcgga ttgagacacc ttttttttca ttagcgtaca gactggatga
     2941 acaagaactg attttgtgct cttttgaagc acgtaaccaa acagggctta acggccctgt
     3001 tttatcgctg actcatttgc tcgaagagtt gtatcaccat ttttcgggta ttaagaaaat
     3061 cagcgcgatg aaatctaaga ttggttcaga ttcagaacgt caaaagcgcg aagagttgtt
     3121 taattacttc atcagaaagg gtgccgtgca gcaagaaaca gaag
//
LOCUS       DABSYT020000231         3159 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.233, whole genome shotgun sequence.
ACCESSION   DABSYT020000231 DABSYT020000000
VERSION     DABSYT020000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.233"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..180)
                     /gene="cesF"
                     /locus_tag="ID725_RS25080"
                     /old_locus_tag="pgaptmp_005016"
     CDS             complement(<1..180)
                     /gene="cesF"
                     /locus_tag="ID725_RS25080"
                     /old_locus_tag="pgaptmp_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HGV7853411.1"
                     /translation="MNEKFRTDLAHTFGIALEEQTDVLSFHDNDGHEWILECASQSEI
                     LFFYCYLLNSESIQIN"
     gene            446..1057
                     /gene="map"
                     /locus_tag="ID725_RS25085"
                     /old_locus_tag="pgaptmp_005017"
     CDS             446..1057
                     /gene="map"
                     /locus_tag="ID725_RS25085"
                     /old_locus_tag="pgaptmp_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HGV7853412.1"
                     /translation="MFSPMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQ
                     SNFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVI
                     SKETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFN
                     TQTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
     gene            1481..3157
                     /gene="tir"
                     /locus_tag="ID725_RS25090"
                     /old_locus_tag="pgaptmp_005018"
     CDS             1481..3157
                     /gene="tir"
                     /locus_tag="ID725_RS25090"
                     /old_locus_tag="pgaptmp_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HGV7853413.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQT
                     STSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATE
                     TATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAI
                     ENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQ
                     TTTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSS
                     IGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLL
                     GNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV"
BASE COUNT          947 a          608 c          737 g          867 t
ORIGIN      
        1 attaatctgt attgattcgc tattcaataa ataacaataa aaaaacagaa tttcagattg
       61 agatgcacac tccagtatcc attcatggcc atcgttatcg tggaaagaga gcacgtcagt
      121 ttgctcttca agagctatcc caaacgtatg tgcaaggtct gtgcgaaatt tctcattcat
      181 attgtagtcc tgccattctt ttcatgttga tatactcaaa cgtaattaat ccgagagttc
      241 tgatacaaat aataaggcgt agatataaga ttacgggaat agtgttgcat attcatgata
      301 gcatatgata aatgtagtgc aactacctgt agataaggcc ggaggataat tcaaatataa
      361 atacaagata attattcttt aaagttattt tttaatggga agcaattttt ttactgtcga
      421 aatataattt ataaaggttt atggtatgtt tagtccaatg acaatggcag gcagatcgtt
      481 agttcaggcg actgcacaga cgcttaaacc tgcggttacc agagctgcaa tgcaagcggg
      541 tacgggagcc acgggtatga ggttcatgcc cgtgcagtcg aactttgtga ttaatcatgg
      601 caaactgact aaccaactcc tgcaggctgt agccaaacaa actcgtaatg gcgataccca
      661 acaatggttc cagcaggagc agacgactta tatatccaga acagtaaata gaactctgga
      721 tgactattgc aggagtaata attcggtcat tagtaaggag actaaaggtc acatttttag
      781 agctgttgaa aatgcgttac agcaacctct tgatatgaac ggggcacaat catcaattgg
      841 tcacttcttg cagtccaata aatactttaa tcagaaagtt gatgagcagt gcggcaaaag
      901 agtggatcca ataacacgtt ttaacaccca gacgaagatg atagaacaag tctcacaaga
      961 aatatttgaa cgcaacttta gcggcttcaa agtcagtgag ataaaagcga ttactcagaa
     1021 tgcgatttta gagcatgtac aggatacccg attgtaggga taaccttgtc aggtataaat
     1081 taagcttcct ggtgtatagc atggcctttg ttgatgtaag aaaaaacgcg ataaagaaac
     1141 ttaataaact ttattattaa ttaagattta tagtttgctt aattggtttt ctttggctgt
     1201 gttttttaat ataaatgaat gtttgctgca attttattta tgaaatgttt tattaagatt
     1261 tcacgtggtg tttactttta tataatataa ttatttttat tttgttaccc gatgtgtgtt
     1321 tttagcacat tattttcttg catcaaaaat cagattgtga tttatttggt cgatgtcatg
     1381 ttcagccgtt tatcgactac gtgctgtata ccatacatta ttatgtaata gcaaaataaa
     1441 ggattgttat ctatttacat aaaataaaaa ggagatattt atgcctattg gtaatcttgg
     1501 tcataatccc aatgtgaata attcaattcc tcctgcacct ccattacctt cacaaaccga
     1561 cggtgcaggg gggcgtggtc agctcattaa ctctacgggg ccgttgggat ctcgtgcgct
     1621 atttacgcct gtaaggaatt ctatggctga ttctggcgac aatcgtgcca gtgatgttcc
     1681 tggacttcct gtaaatccga tgcgcctggc ggcgtctgag ataacactga atgatggatt
     1741 tgaagttctt catgatcatg gtccgctcga tactcttaac aggcagattg gctcttcggt
     1801 atttcgagtt gaaactcagg aagatggtaa acatattgct gtcggtcaga ggaatggtgt
     1861 tgagacctct gttgttttaa gtgatcaaga gtacgctcgc ttgcagtcca ttgatcctga
     1921 aggtaaagac aaatttgtat ttactggagg ccgtggtggt gctgggcatg ctatggtcac
     1981 cgttgcttca gatatcacgg aagcccgcca aaggatactg gagctgttag agcccaaagg
     2041 gaccggggag tccaaaggtg ctggggagtc aaaaggcgtt ggggagttga gggagtcaaa
     2101 tagcggtgcg gaaaacacca cagaaactca gacctcaacc tcaacttcca gccttcgttc
     2161 agatcctaaa ctttggttgg cgttggggac tgttgctaca ggtctgatag ggttggcggc
     2221 gacgggtatt gtacaggcgc ttgcattgac gccggagccg gatagcccaa ccacgaccga
     2281 ccctgatgca gctgcaagtg caactgaaac tgcgacaaga gatcagttaa cgaaagaagc
     2341 gttccagaac ccagataatc aaaaagttaa tatcgatgag ctcggaaatg cgattccgtc
     2401 aggggtattg aaagatgatg ttgttgcgaa tatagaagag caggctaaag cagcaggcga
     2461 agaggccaaa cagcaagcca ttgaaaataa tgctcaggcg caaaaaaaat atgatgaaca
     2521 acaagctaaa cgccaggagg agctgaaagt ttcatcgggg gctggctacg gtcttagtgg
     2581 cgcattgatt cttggtgggg gaattggtgt tgccgtcacc gctgcgcttc atcgaaaaaa
     2641 tcagccggta gaacaaacaa caacaacaac tactacaact acaactacaa gcgcacgtac
     2701 ggtagagaat aagcctgcaa ataatacacc tgcacagggc aatgtagata cccctgggtc
     2761 agaagatacc atggagagca gacgtagctc gatggctagc acctcgtcga ctttctttga
     2821 cacttccagc atagggaccg tgcagaatcc gtatgctgat gttaaaacat cgctgcatga
     2881 ttcgcaggtg ccgacttcta attctaatac gtctgttcag aatatgggga atacagattc
     2941 tgttgtatat agcaccattc aacatcctcc ccgggatact actgataacg gcgcacggtt
     3001 attaggaaat ccaagtgcgg ggattcaaag cacttatgcg cgtctggcgc taagtggtgg
     3061 attacgccat gacatgggag gattaacggg ggggagtaat agcgctgtga atacttcgaa
     3121 taacccacca gcgccgggat cccatcgttt cgtctaaat
//
LOCUS       DABSYT020000232         3118 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.234, whole genome shotgun sequence.
ACCESSION   DABSYT020000232 DABSYT020000000
VERSION     DABSYT020000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.234"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..422)
                     /locus_tag="ID725_RS25095"
                     /old_locus_tag="pgaptmp_005019"
     CDS             complement(<1..422)
                     /locus_tag="ID725_RS25095"
                     /old_locus_tag="pgaptmp_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGV7853414.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDR
                     AWEVFEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLV"
     gene            complement(678..1436)
                     /locus_tag="ID725_RS25100"
                     /old_locus_tag="pgaptmp_005020"
     CDS             complement(678..1436)
                     /locus_tag="ID725_RS25100"
                     /old_locus_tag="pgaptmp_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGV7853415.1"
                     /translation="MTTKISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLRVTLSNSQNLQPSLRGL
                     QISPKARSLILWTERGAARHAKMLETDRAWEVFEKLEDCYFSQKIPEQLQLPESTLSI
                     NYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMRILNELHSKGYNVDAAVAE
                     FNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            complement(1711..1893)
                     /locus_tag="ID725_RS25105"
                     /old_locus_tag="pgaptmp_005021"
     CDS             complement(1711..1893)
                     /locus_tag="ID725_RS25105"
                     /old_locus_tag="pgaptmp_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGV7853416.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(1890..2417)
                     /locus_tag="ID725_RS25110"
                     /old_locus_tag="pgaptmp_005022"
     CDS             complement(1890..2417)
                     /locus_tag="ID725_RS25110"
                     /old_locus_tag="pgaptmp_005022"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGV7853417.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGLGVRRAA"
     gene            complement(2414..2860)
                     /locus_tag="ID725_RS25115"
                     /old_locus_tag="pgaptmp_005023"
     CDS             complement(2414..2860)
                     /locus_tag="ID725_RS25115"
                     /old_locus_tag="pgaptmp_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGV7853418.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGKAA"
     gene            complement(2817..3053)
                     /locus_tag="ID725_RS25120"
                     /old_locus_tag="pgaptmp_005024"
     CDS             complement(2817..3053)
                     /locus_tag="ID725_RS25120"
                     /old_locus_tag="pgaptmp_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HGV7853419.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RVAWAPSVAAATEKWNRRAGDEANLSASQRSNQK"
BASE COUNT          716 a          776 c          750 g          876 t
ORIGIN      
        1 acaaggctgt ctgtttcttt tgcggaaagc ccgttgagct tcttctcttg tttgccagta
       61 tttttctcgc actggctgaa atagcagtct tccagttttt cgaacacttc ccacgcccga
      121 tcggtttcga gcatttttgc gtgacgggct gcgccgcgtt ctgtccagag gatgagggag
      181 cgaacgttac gggcgatttt cacagaaggt cttaaagaca tgctgtgctt aaattcgcgc
      241 aactcctcat tctcaagtaa aaagtagtgc ttaccgataa caaatcgtcc agaattccga
      301 ttgaaatttt ttcggatgta atctggttct gtgccatata agctggcaag aagctctgta
      361 gtcaccacag gtatattact aaatgaaatt atggagaggg actccacgga gatggttttc
      421 atgctttatg tcctacttga tattttgatt tacccctttt gagagggtga ccgggcgctc
      481 aaaaccgcaa gtagtcggcg ggcatattcc cattgctggt gttgtattag ccgcacgccc
      541 ggtcataaac caagaagact ggacacaaaa aaaccgcatg tctgtcgggc gcggtaaccg
      601 ctacttgcta aggtgttttg agcaccatga ggcggaatat atccccgtta atgcagcatt
      661 gtcaaacgat ctgttcttta aagacttaac gagaagcctt ttcgggaatt tcgatctgac
      721 aatcttgaaa tatcctgtag agtacggcgc atttcttccg tcagatgctt gaaggcgtta
      781 aattcggcga cagctgcatc gacgttatag cccttgctgt gtagctcgtt gaggatgcgc
      841 atagttgggc ttggcatatc gaagagcaca gaaacgtcca ggttaagagt ttttcgatca
      901 acatagctca tcatggagta ggggtgatgc tctgaaaacc acgagagagg gtagttgata
      961 gatagtgttg attctggaag ctgaagttgt tctgggattt tttgactgaa atagcagtct
     1021 tccagttttt cgaacacttc ccacgcccga tcggtttcga gcatttttgc gtgacgggct
     1081 gcgccgcgtt ctgtccagag gatgagggag cgggctttcg gggaaatttg taaccctctt
     1141 aaagatggtt gcaaattttg tgagttactt aaagtaaccc gcaaattttg tgagttactt
     1201 aaagtaaccc gcaaattttg tgagtagttt aaagctaccc gcaattcttt aaggtcatta
     1261 ccaacaactt tgaaaaagtg tttcccttca acgaagcgta ctttgttctc atgatgattc
     1321 tggcgaatac gcaccggctc agtgccgtaa agctgcgcca aaagttcggt ggtaataaca
     1381 ggaatctggt tatgggtgat cggggagaga gtttcaacag aaattttagt tgtcataatg
     1441 acgccctctg gtggtttctt aataactcac caccgacgac gccaatcgtc tggtggtgaa
     1501 ctgtgcaggg ttggcgtaac cgggaaaccg accggcgcgg atctctccgc ccccacacag
     1561 cccaccataa ttcagatgtg cgcgtgcata cgacaataaa aaacacgctc gcggcgtgta
     1621 tctgtcgcgg tctctatcca ggacgccaat cccgacgcca gattttgctg gcgtttgagg
     1681 aatatagccc cgaataaatc atcacgtcaa tcaccttgtt ttcctcgcac gatgtcttag
     1741 ccaccggata tcccacaggt gagccgtgta attgaaggtt tttacgtcag attcttttgg
     1801 gattggcttg cgtttatttc tggagcgttt cgttggaagg tatttgcagt tttcgcagat
     1861 gatgtcggtg atacttcgtc gctgtcgcct catgcagccc tcctgacgcc cagcccgatc
     1921 gccatcaatg ccgctttgga tacggtagta aacatccgtc gaggactgat gaacggtcgc
     1981 caaatcagca acattgagcc tttgctgttt cccttcttct ccagccctgt cgatggttcg
     2041 ataaaattaa tccgtccatc agtgatgatg cgaacttcgt caacactctc cagagccttg
     2101 ctgaaccatc cgacagacat atcctctggc acaagcatca ctaccgtctg tcgctgttgt
     2161 atgcactgct cagcggcttt ttccacccac ggcctgatat tgctgtacgg tgggttattc
     2221 cagattgcac cgtggcttat ccactcagaa tttagcgcgt cgtcagcctc agttagccag
     2281 tgagcgcaca gagcgttttt gtcgctcgca gctgaatcca gccagaatcc aaactcaata
     2341 tccagcgcat caaaaagcca gagcggcgtt tgccagcagt ccttgtcgtg tgctggcgta
     2401 tttgatttga tagtcatgca gccttccctt ttcgttgtga ccattcatac tctcgccggg
     2461 agtcatcact ccaccgcacg ttgcgctctg agccgaacca gaacatgatt tcgataagct
     2521 cagtcatgct ggcctttcgc attttgctgg tacgcacgcc aagcatgaca acgccaccgt
     2581 cgataccagg cgcacttctt tgctccagtt ttttggtctt aagccacagg gcagtgaaca
     2641 ggtctttcca gtcttccggc gccagccgtt gaccatgcca tagcacctga cgcgaaacat
     2701 cgttcagcat cggccacatg cggtcattct gcgctttgct gcgcttgggt tctttaacgt
     2761 ggacttcgtg gggtgacttg tcgtcgatgg gtagtgagag aatggcgtct atggcgttat
     2821 ttctgattgc ttcgttgcga agcagaaagg tttgcttcat ctcctgctct ccggttccat
     2881 ttttcagtag ccgcagcaac tgatggtgcc catgccaccc tggcttcaca gaggtcacat
     2941 tctgcatagc cccacacatc aatatttatt ccggcctcaa cccacagacg agcattaccg
     3001 ccgcaaaacg gacattcttt tagctttggc tgggttaatg ataggtcgct catgctcact
     3061 ccttcactta aaatccagac tccggataat tctgttgcgc tgaaactcat tgttgagt
//
LOCUS       DABSYT020000233         3102 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.235, whole genome shotgun sequence.
ACCESSION   DABSYT020000233 DABSYT020000000
VERSION     DABSYT020000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.235"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..79
                     /locus_tag="ID725_RS25125"
                     /old_locus_tag="pgaptmp_005025"
     CDS             <1..79
                     /locus_tag="ID725_RS25125"
                     /old_locus_tag="pgaptmp_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853420.1"
                     /translation="VCREWSVTDNARYSDFSCTIEQVVN"
     gene            79..777
                     /locus_tag="ID725_RS25130"
                     /old_locus_tag="pgaptmp_005026"
     CDS             79..777
                     /locus_tag="ID725_RS25130"
                     /old_locus_tag="pgaptmp_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGV7853421.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELRDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYHGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(794..1048)
                     /locus_tag="ID725_RS25135"
                     /old_locus_tag="pgaptmp_005027"
     CDS             complement(794..1048)
                     /locus_tag="ID725_RS25135"
                     /old_locus_tag="pgaptmp_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGV7853422.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            1158..1268
                     /locus_tag="ID725_RS25140"
                     /old_locus_tag="pgaptmp_005028"
     CDS             1158..1268
                     /locus_tag="ID725_RS25140"
                     /old_locus_tag="pgaptmp_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGV7853423.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            1571..2449
                     /locus_tag="ID725_RS25145"
                     /old_locus_tag="pgaptmp_005029"
     CDS             1571..2449
                     /locus_tag="ID725_RS25145"
                     /old_locus_tag="pgaptmp_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HGV7853424.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSPHHY"
     gene            2503..>3102
                     /locus_tag="ID725_RS25150"
                     /old_locus_tag="pgaptmp_005030"
     CDS             2503..>3102
                     /locus_tag="ID725_RS25150"
                     /old_locus_tag="pgaptmp_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGV7853425.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLP"
BASE COUNT          799 a          667 c          874 g          762 t
ORIGIN      
        1 tgtgtgccgg gagtggagcg tcacggataa cgcccggtac agtgatttca gctgtacgat
       61 tgagcaggtg gtgaactgat gcaggacatt catgaagaaa gtctgaacga gtcggttaaa
      121 tcagagcagt caccgcgggt ggtgctctgg gaaattgacc tgacggtgca gggcggtgag
      181 cggtattttt tctgtaatga gctgaatgaa aaaggggagg cggtcacctg gcaggggcgg
      241 caatatcagg tatacccgat tgacggcagt ggctttgaga tgaacgggaa gggcagcagt
      301 gcccgcccgt cgctgacggt gtcgaatctg ttcggtctgg tcaccggaat ggcggaggac
      361 ctgcagagcc tggtgggggc cacggtggtc cgccgccggg tgtatgcccg ttttctggat
      421 gcggtgaatt ttgtggcggg caatccggaa gcggacccgg agcaggagct gagagaccgc
      481 tgggtggtgg agcagatgtc agagctgacg gccatgacag cctcgtttgt gctggcaaca
      541 ccgacggaga cggacggagc gctgtttccc ggtcgcatca tgctggcgaa cacctgtatg
      601 tgggattacc ggggagatga atgcggttat cacggtcctg cggtggcgga tgagttcgac
      661 aaccccacca cggatatccg taaggacaga tgcagcaagt gcatgcgcgg gtgtgagatg
      721 cgcggcatgg tggctaattt tggcggtttc ctttccatta ataaactttc gcagtaatgg
      781 attatgccca ccatcaggtg ggttttttat cttcgccgta catgctgctg agagtcttaa
      841 gaagagcctc cctaaatttt tcagattctt gttgagcaaa accttctatt gatcttggtg
      901 atttttcttc atcaatagca gcttgcaaga tcataactat ttctgaattt aatgagcgtc
      961 cgtttttcga tgctcttatg gtcaggtcgc gcttgaggct atctggcatt cttacgctat
     1021 atggggctat gtctctaacc ttcgtcatac ttacaccgtg ataatcacat tgatatcaca
     1081 gtgtattcaa aaaaactttg acttgatata gtcacagtga tacgtttgtg attcataata
     1141 actcacgcgg tggcgatatg gaaaaagaag ttagcagaat tttggtgaga atacctcagt
     1201 cgctaaaaga tgcgattaca ggaaaggcca aagaagaatg ccggtcgttt aactcggaag
     1261 tgatgtgatt gctggacagc ctgaagagag aggggataac ggtatgagta aagaatgttg
     1321 ttttttgcgg cattagcgaa tcagacgctg atcaaacata catttactct aaagaaacag
     1381 gtcggatgct gtgtagtgac tgcgtgtttg acatcataag atacaagtat cttggatgtt
     1441 ctgccagcat tagcaatata ggtgaagtgt acgaagggaa agatataact gatagagcag
     1501 aaagttgaag ccccaactgc tgtaacagtc agggcttcgt tatcaacaaa tcggcttagg
     1561 aaatattgac atgaaaagta tagcaaaggc acaaaacgat ttcactatct tcaaattcgg
     1621 cgacagtgaa atccgcgtca tcaacaagtg cggtgagccg tggtttgtag ctaaagatgt
     1681 ttgtgatgct ttagctttga ctaactcacg caaggcgctt actgcacttg atgacgatga
     1741 aaagggagta actttaagtt acacccttgg tggtgagcag aatctaagca ttgtgagcga
     1801 atcaggtatg tatacattgg ttctgcgctg ccgcgatgca gtcaataaag gttcagtccc
     1861 gcacaaattc cgcaagtggg taacagcaga agttctgcct tcaattcgca aacatggcga
     1921 gtatgtaaaa ggaaagaaaa ccactgttga ggaaagaaca ccgctacgcg atgcagtaaa
     1981 catgctggta ggaaagaaag gacttcgcaa tgacgatgca tacaatatgg ttcatcagcg
     2041 ttttggtatt gacagcattg atgagctttc aattgaacaa atcccgcttg ccgtagagta
     2101 catccacagg gtagtgcttg aaggtgaatt cattggcaaa caagagaaga gcaccaacga
     2161 gctttccgca aaagaagcaa acagccttgt atggctatgg gattatgcca accgctcaca
     2221 ggcattattc cgcgaactgt atccggcgct aaaacaaatt caatcgaact attccggcag
     2281 atgctacgac tacggtcatg agttctcgta tgttattgga atggcgagag acgttttaat
     2341 taatcacaca cgagatgttg atatcaatga gccagacgga ccaacgaatc tttccgcatg
     2401 gatgagactt aagaataaag aattacctcc ttcaccgcat cactactaac agattgccaa
     2461 cgaaatgacc cagcttcggc tgggttttta tcaggagttc tcatgctcta tagcaatata
     2521 ttggcgcacg cccggcgatg tgcaccagcg gagtcgtgcg gcttcgtggt gagaacgccg
     2581 gaaggggaaa gatattttcc ctgcgtgaat atctccggtg agccggaaga ttatttccgg
     2641 atgtcgccgg aggactggct gcgggcagaa atgcaggggg agattgtggc actggtccac
     2701 agccaccccg gtggtctgcc ctggctgagc gaggccgacc ggcggctgca gataaaaagt
     2761 gcactgtcct ggtggctggt ctgccggggg gaaattcata aattccgctg tgtgccacat
     2821 ctgacaggac ggcgctttga gcacggggtg acggactgtt acacgctgtt ccgggatgcc
     2881 taccatctgg cgggaattga tatgccggat tttgagcgtg aggatgactg gtggcgcaac
     2941 ggtcagaacc tttacctgga caatatggcg gtcaccggct tttaccgggt gcccctgtcc
     3001 tctgcacagg cgggcgatat cctgctgtgc tgctttggcg catcggtggc caatcatgcc
     3061 gccatatact gcggcaacgg tgaactgctt caccatctgc ct
//
LOCUS       DABSYT020000234         3094 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.236, whole genome shotgun sequence.
ACCESSION   DABSYT020000234 DABSYT020000000
VERSION     DABSYT020000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.236"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            28..525
                     /gene="ftnA"
                     /locus_tag="ID725_RS25155"
                     /old_locus_tag="pgaptmp_005031"
     CDS             28..525
                     /gene="ftnA"
                     /locus_tag="ID725_RS25155"
                     /old_locus_tag="pgaptmp_005031"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGV7853426.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(562..801)
                     /locus_tag="ID725_RS25160"
                     /old_locus_tag="pgaptmp_005032"
     CDS             complement(562..801)
                     /locus_tag="ID725_RS25160"
                     /old_locus_tag="pgaptmp_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGV7853427.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            993..2204
                     /gene="tyrP"
                     /locus_tag="ID725_RS25165"
                     /old_locus_tag="pgaptmp_005033"
     CDS             993..2204
                     /gene="tyrP"
                     /locus_tag="ID725_RS25165"
                     /old_locus_tag="pgaptmp_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGV7853428.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2266..2931)
                     /gene="yecA"
                     /locus_tag="ID725_RS25170"
                     /old_locus_tag="pgaptmp_005034"
     CDS             complement(2266..2931)
                     /gene="yecA"
                     /locus_tag="ID725_RS25170"
                     /old_locus_tag="pgaptmp_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGV7853429.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT          716 a          773 c          747 g          858 t
ORIGIN      
        1 caaatataac ctttgtggag cactatcatg ctgaaaccag aaatgattga aaaacttaat
       61 gagcagatga acctggaact gtactcttca ctgctttatc agcaaatgag cgcctggtgc
      121 agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca ggaagagatg
      181 acgcatatgc agcgtctgtt tgattacctg actgataccg gcaatttacc gcgtattaat
      241 accgttgaat ctccgtttgc tgaatattcc tcacttgatg aattattcca ggaaacctat
      301 aaacacgaac aattaatcac ccagaaaatt aacgaactgg ctcatgctgc aatgaccaat
      361 caggactacc caacatttaa tttcctgcag tggtatgttt ctgagcagca tgaagaagag
      421 aaactgttca aatcgattat tgataaatta agcctggcag gcaaaagcgg cgaaggtctg
      481 tattttatcg acaaagaact ctctaccctc gacacacaaa actaatgctc tccggcggca
      541 ggtttctgcc gccatttcaa atcagtgacg gcaaatctta ctctgatcgg tcgaaaaacc
      601 ttcttctgaa ggtataaatt tgccttttgc tgccagaaac gctaccagct ctcccgctgt
      661 catcccttct gccgagcaag tgtgaaaacg tgcctgttca ccaaaacgcg ctttaatcgc
      721 agcttccaga ctggcatgcg tatattgctc gcctgattca atcatcatat ttaacacttc
      781 atgaccgtga atagagtcca tcgttcctcc tcaaaaaaaa gcctagcgta gcgattgccg
      841 cttatgaaga ctttgcgcca gcgcaggact gaatgctttt tattgtacat ttatgtttac
      901 accatatgta acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg ccgcagatat
      961 cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa cagaaccctg ggaagtgttt
     1021 ttatcgtggc gggaaccaca attggcgcag gcatgctggc aatgccgctg gctgcggccg
     1081 gtgttggttt tagcgttacg ttaatcttgt tgattgggct ttgggcgttg atgtgctaca
     1141 cggcgctatt actgctggag gtgtaccagc atgttccggc agataccggt ctgggcacgc
     1201 tggcaaaacg ctatctggga cgctacggtc aatggctgac gggcttcagt atgatgttct
     1261 taatgtatgc tctgactgcg gcatacatca gcggtgccgg tgaattgttg gcctccagca
     1321 tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg cgtgttgttg ttcacttttg
     1381 ttgccggtgg cgtggtttgt gtcgggacat cgctggtcga tttatttaac cgttttctgt
     1441 tcagcgccaa aattattttt ctggtggtga tgctggtatt gctgctaccg catattcaca
     1501 aagtgaatct tttaaccctg ccgttgcaac aggggctggc actgtctgca atcccggtga
     1561 tttttacgtc gtttggtttt cacggtagcg tgccgagtat tgtcagctat atggatggca
     1621 acattcgtaa gctacgctgg gtgtttataa ccggtagtgc gatccccctg gtggcatata
     1681 ttttctggca ggtggcgacg cttggcagca ttgattcaac aacctttatg ggattgctgg
     1741 ctaatcatgc cggattaaac gggctgttac aggcgttacg cgaaatggtg gcctctccgc
     1801 atgttgagct ggcagtgcat ttatttgctg atttagccct tgccacgtca tttctcggcg
     1861 ttgcgttagg cttatttgat tatctggcag atttgtttca gcgttcaaat accgttggtg
     1921 gacgattgca aactggagca attacctttc tgccaccgtt ggcgtttgca ctgttttatc
     1981 cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc gctggcggta ctggcattga
     2041 ttatcccttc gctgttgacc tggcaaagca gaaagcacaa tcctcaagcg ggttaccggg
     2101 tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg tggtattgct gtgattggcg
     2161 tgcaattttt gattgcggca gggttgttac cagaagtggg gtgattagat agcctcaaat
     2221 tccttattgg gtgccagaat taacgctgac acccaatttg acttcttaat gcaggcagca
     2281 ctgcttaaat tttttaccac taccgcacgg gcaaggatcg ttacgccccg gtttctcttc
     2341 tgctttgatc ggttgctgaa cagctttttc ctgcggatgc gccatccagt acgcatgtag
     2401 atcaagcgcc gccagtcgaa tggcatctac gctctcttca aacgcttctg gcgacatctt
     2461 ctctacccgc tcgaagtttt cctcagtacc gtgcagcgct atcgcctcca gcgctggttt
     2521 taacgaatcg ggcaatgttg accagtcaga aagtgccacg ccccgcatat agccaaagca
     2581 ccactcctca acaatcgtca gctcgctgcc atcaacttct cgcaagccga ataacggctc
     2641 aaactgctcc gggaattcgt tcagacgctc tgcggtatcg gccatatgtt gaaaagccag
     2701 gttcataaag cgcgtcatct ctttctctga cgcccagcgc ggcacatagt cagccccacc
     2761 ccacacggca accagccact gtgccggttc aatctcttgc ggagaactca acaccgccgt
     2821 caataaaccg tccagctccg ccacatcaag gatggcgtgg tcagtgttgt atttggtcag
     2881 aatatcgtcc agccattcca actcactttc gtttaacggt cccgttttca tacgcttttc
     2941 cttgtggatc tcaactcgcc agcacctatc ttacatgccg gtccgtatca gagatacttt
     3001 ttgagtggct ttgccggtga ttaaaaatca aggagggtgt aacgacaagt tgcaggcaca
     3061 aaaaaaccac ccgaaggtgg tttcacgaca ctgc
//
LOCUS       DABSYT020000235         3083 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.237, whole genome shotgun sequence.
ACCESSION   DABSYT020000235 DABSYT020000000
VERSION     DABSYT020000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.237"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            198..1151
                     /gene="ompT"
                     /locus_tag="ID725_RS25175"
                     /old_locus_tag="pgaptmp_005035"
     CDS             198..1151
                     /gene="ompT"
                     /locus_tag="ID725_RS25175"
                     /old_locus_tag="pgaptmp_005035"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGV7853430.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1338..2822
                     /locus_tag="ID725_RS25180"
                     /old_locus_tag="pgaptmp_005036"
     CDS             1338..2822
                     /locus_tag="ID725_RS25180"
                     /old_locus_tag="pgaptmp_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGV7853431.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT         1020 a          647 c          596 g          820 t
ORIGIN      
        1 caaaaagaaa atttccagca tattaaacca caacaataat acaacacttg gcaagctgcc
       61 cataaagaat aaataaaacg aaacaataat tcaaacttaa acaaaccata aacagttaaa
      121 caatatacaa ttgactcatt aagttagata taaaaaatac atattcaatc attaaaacga
      181 ttgaatggag accttttatg cgggcgaaac ttctgggaat agtcctgaca acccctattg
      241 cgatcagctc ttttgcttct accgagactt tatcgtttac tcctgacaac ataaatgcgg
      301 acattagtct tggaactctg agcggaaaaa caaaagagcg tgtttatcta gccgaagaag
      361 gaggccgaaa agtcagtcaa ctcgactgga aattcaataa cgctgcaatt attaaaggtg
      421 caattaattg ggatttgatg ccccagatat ctatcggggc tgctggctgg acaactctcg
      481 gcagccgagg tggcaatatg gtcgatcagg actggatgga ttccagtaac cccggaacct
      541 ggacggatga aagtagacac cctgatacac aactcaatta tgccaacgaa tttgatctga
      601 atatcaaagg ctggctcctc aacgaaccca attaccgcct gggactcatg gccggatatc
      661 aggaaagccg ttatagcttt acagccagag gtggttccta tatctacagt tctgaggagg
      721 gattcagaga tgatatcggc tccttcccga atggagaaag agcaatcggc tacaaacaac
      781 gttttaaaat gccctacatt ggcttgactg gaagttatcg ttatgaagat tttgagctag
      841 gtggcacatt taaatacagc ggctgggtgg aagcatctga taacgatgag cactatgacc
      901 caggaaaaag aatcacttat cgcagtaaag tcaaagacca aaattactat tctgtttcag
      961 tcaatgcagg ttattacgta acacctaacg caaaagttta tgttgaaggc acatggaatc
     1021 gggttacgaa taaaaaaggt aatacttcac tttatgatca caatgataac acttcagact
     1081 acagcaaaaa tggtgcaggc atagaaaact ataacttcat cactactgct ggtcttaagt
     1141 acacctttta acgaagttaa ccagattttc tccccggtgg tattgtataa ccccggggaa
     1201 tttgtttaca gttacaagta gacaatatcc actaattttg taagcattta acctatgtca
     1261 taacgtgtaa tctcttaccg caacacaaga cctcaatata ttcaaatttt atctttattc
     1321 cgatattcag agacacaatg cgaaaaataa ttactcattt caaagttgtt ttaacgttac
     1381 ttctaccagt aaccgtatct gcccagcaga tacagtggca atcctgtatg gccagtcaat
     1441 tcaaccactg gtttggtgag gaaaaaccgt ctcctgactt actatgtggt tatttgtctg
     1501 ttccattaaa atatacagac acaggcggag atgcttctta tgaaaaaaaa tcacaagtca
     1561 aactagcgtt gacaaaattg ccggcaaaaa gcaagcataa aggaagtatc ctgataataa
     1621 gtggtggtcc cgggttacca ggcataaatc cttatattaa ctttgactgg ccagtcacaa
     1681 atcttcgtga gtcatgggat attattggat ttgatcctcg aggcgtcggg cagtccactc
     1741 cgacaataaa ctgccggcaa tcagatacag agactcagga aaacataacc gaaaagcaac
     1801 aagtattaaa taaaattaat gcctgtatcc ataataccgg agccgaagtc attcgccata
     1861 tcggctctaa cgaggctgta tacgatattg atcgtattag gcaagccttg ggggataaac
     1921 aactgacagc cgtggcgtat tcgtatggaa ctcaaattgc agccttatat gcagaacgtt
     1981 ttccctacaa cgtaagatct atcgttcttg atggagtcgt cgatatcgat gacctggagg
     2041 acaacttcac atggcaactc aaacaggcac agagttatca ggaaacgttt gatcgctttg
     2101 catcctggtg tgcgcgtaca aaaagttgcc cgctttcttc agacagagat aaggcaataa
     2161 ctcagttcca tgagctatta tcaaaattac atcacaaacc tttattagac agtaagggag
     2221 aaaatatatc ttcagatgaa ctcatatcat taacaacaga ccttctgcta tggcgttcat
     2281 catggccaac ccttgcaact gccatacgcc agttctctca ggggattgtc agtaatgaaa
     2341 ttgaaactgc gctcagtgct ccgatagcct cagaagagtc aagtgatgct tcgggggtaa
     2401 tcctttgtgt agatcagggg gatgagcaat taacaccaga agagcgaaaa ttccgaaaag
     2461 aggctcttgc gaatgccttc ccggctatta actttgacaa tggacgttcc gattcacctg
     2521 atttttgtga attatggcca atacatagcg acctgaacaa aactcgcctg aaaaatactg
     2581 ttctgccctc tggtttactg tttgtagcac acaaatacga cccaacaacg ccctggatta
     2641 atgcccgtaa gatggcagag aaattttcca gcccgttact aacaataaat ggtgatgggc
     2701 atacattagc tctcaccgga gttaatttat gtgtagataa agcagttgta catcacctga
     2761 tcactccaca aaaaatagaa aatatatact gcccaggaaa ttctgaagca gaaatacaat
     2821 aatttcaaaa tattccgcta tttgctccca atgcaaaaca tatattgcat tgggagcata
     2881 ttcatatttt tgttattctt gtatgaaatc gtctcatgta cacggtcctt ttttcgccga
     2941 aaactgatat tactttaatc aaaagttgac gtgccagatt cgaagtaaat ttataaaata
     3001 tcaaatcaat atacattttg caccttcgag atagtaatag ccattactgt aacatgagtt
     3061 acaaacagct tcatacccag agc
//
LOCUS       DABSYT020000236         3070 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.238, whole genome shotgun sequence.
ACCESSION   DABSYT020000236 DABSYT020000000
VERSION     DABSYT020000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.238"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(160..849)
                     /locus_tag="ID725_RS25185"
                     /old_locus_tag="pgaptmp_005037"
     CDS             complement(160..849)
                     /locus_tag="ID725_RS25185"
                     /old_locus_tag="pgaptmp_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGV7853432.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQPTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            954..1184
                     /locus_tag="ID725_RS25190"
                     /old_locus_tag="pgaptmp_005038"
     CDS             954..1184
                     /locus_tag="ID725_RS25190"
                     /old_locus_tag="pgaptmp_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGV7853433.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAHQIRPDLPKLFPHTEKNAA"
     gene            1254..1793
                     /locus_tag="ID725_RS25195"
                     /old_locus_tag="pgaptmp_005039"
     CDS             1254..1793
                     /locus_tag="ID725_RS25195"
                     /old_locus_tag="pgaptmp_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HGV7853434.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAEDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            1790..2809
                     /locus_tag="ID725_RS25200"
                     /old_locus_tag="pgaptmp_005040"
     CDS             1790..2809
                     /locus_tag="ID725_RS25200"
                     /old_locus_tag="pgaptmp_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGV7853435.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNALIAGGIMANTAEIFNFPVPD
                     VAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKDVYPNQVNTKDKITKDNIKPYSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKSKLDLTNTDWI
                     YGVDL"
     gene            2806..>3070
                     /locus_tag="ID725_RS25205"
                     /old_locus_tag="pgaptmp_005041"
     CDS             2806..>3070
                     /locus_tag="ID725_RS25205"
                     /old_locus_tag="pgaptmp_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGV7853436.1"
                     /translation="MKNIAAQMVNFDCEQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     RQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAG"
BASE COUNT          890 a          714 c          720 g          746 t
ORIGIN      
        1 tataatcttt ttttaaagag tcaaaaaatg ggtcattgtg atcaaaatca gagaatttct
       61 catattttaa agtatccatt aagttctcca ttctaaaatg aataattaag ttgttttact
      121 gaatgagcac aatgcccggt tgataatttt taattggtac tatccatgct tcctatacgt
      181 ctgcggcatg ctcccaataa ctttcccgaa gataaacacc cggttcatct cgtctttctc
      241 gattgggtcc cacggtgagt agctcttgtt atcagagata accagcagtt tatccttcat
      301 catttgcagg cgctttacat gggctgtgtc gtcgtacaga aacgcataga taccatcacc
      361 gtcgaaagat ttaaccgtga tatcaacgaa cagcagatca ccaggttcaa tcgttcctga
      421 catgctgtca ccgcgtacgt taatgatgcg gatattttct gccttcctgc catcgaacat
      481 gtgacgagca tcgtcaaacg agtactcaac cgagcgtagg acttctacaa actcacggtt
      541 gatgacaccc ggcccggcac tcacttctat atcaagaacg tcaatcttaa agtatttgga
      601 atggctgaca gttggttgta ttggttgcac tgtactgtct gacatatttc caacgccaga
      661 agataaccat tctgcgcgca cacccaaagc gttcgcgatc tccacgattt tagttgtttg
      721 attagctttc cctgtttcga ttttctgaat agcagcttgg ctaaccccga ccaaatcccc
      781 aagcgccttt tgtgtaaggc ctcgcgctaa tctggcttct ttaagtcttt ctgagagtgt
      841 tgttttcata gttcaaatgt acaaccaagg ttttattcca tcaaacgaaa atggttgttg
      901 actaaaaaca accatagttt taatcttgat tcaaattaac cacggaggtt gttatgaacc
      961 cagctattaa aacagcgatc aatatcgttg gttcacaaaa gaaactgggc gctgcttgcg
     1021 aagtttcaca gcaggccgtc tataagtggc ttcacaacaa agcaaaggta tcccctgaac
     1081 atgtcggcag cattgttacg gctactggtg gagtagtgaa ggcacaccag attcgcccgg
     1141 atcttccgaa gttgtttcca cacaccgaaa agaacgcagc ttaaatttcc atttcacgct
     1201 ctttaacaat aagcaatcaa cttaacagtc aattcaaact aaaggagtca attatgcaac
     1261 cacttacata ccaacagact agcggattta gcccgactgc ggtgataaat cgttctcaaa
     1321 caaaacaggt gccaggccac gaaaaaatcc gtgatgccgt ccgcgcctgg tcggctgaag
     1381 ataatcagga tgtcgttgcc acactcattg tgaatgagta tcgggagcag ggcggcggca
     1441 ccatcgattt ccctgatgat gtcagccgtg cacgccagaa gctgttccgc ttcctcgata
     1501 acaaattcga ttctgaaaaa taccgaaata acgtgcgtga actgaccccg gcaattctgg
     1561 cggtactacc gctggaatat cgcggttacc tggttgagca ggatagcttt atggctaggt
     1621 tggctgaaat ggaaaaggaa ctcagtgagg caaaacaggc tgtcattctc aacgcaccac
     1681 gccaccagaa actgaaggaa attagtgaag gtattgtgtc gatgtttcgt gtggacccag
     1741 atctggctgg tccactgatg gcgatggtca ccaccatgct gggggcaata tgacaggttc
     1801 agaaatggcg aaagccggtc tgctggaaca gaaccgactt tcaggtgcaa atcgtaacgc
     1861 actcattgcg ggaggaatta tggcaaacac tgctgagata ttcaattttc cagtgccgga
     1921 tgtggcacaa aaggagccgc gcgtggcaga tctcgatgat ggttatacgc gcattgcaaa
     1981 tgagttgctg gaagctgtga tgctggccgg attaacacag caccagcttc tggtcttcct
     2041 ggctgtcatg cgcaaaacat atggctttaa taaaaaactg gattgggtga gcaacgagca
     2101 actttccgaa ttgaccggga tattgccgca caagtgttct gctgcaaaaa gcgttctggt
     2161 aaagcgtggg attcttattc agagcgggcg gaataccggc attaataatg tggtcagtga
     2221 atggtcaaca ttacccgaat caggtaagaa aaataaagtt tacctgaaag aggtaaattt
     2281 acctgaatca ggtaaaaaaa gtttacccaa atcaggtaaa gacgtttacc cgaatcaggt
     2341 aaacacaaaa gacaaaataa caaaagacaa tataaaacct tattcgtccg agaattctgg
     2401 cgaatcctct gaccagccag aaaacgacct tcctgtggtg aaaccggatg ctgcgattca
     2461 gagcggcagc aagtggggga cagcagaaga cctgaccgcc gcagagtgga tgtttgacat
     2521 ggtgaagacc atcgcgccat cagccagaaa accgaatttt gcagggtggg ctaacgatat
     2581 ccgcctgatg cgtgaacgtg acggacgtaa ccaccgcgac atgtgcgtgc tgttccgctg
     2641 ggcatgccag gacaacttct ggtccggtaa cgtgctaagt ccggccaaac tccgtgacaa
     2701 gtggacccaa ctcgaaatca accgtaacaa gcaacaggct ggagtgacag ccggaaaatc
     2761 aaaactggac ctgacaaaca ctgactggat ttatggggtg gatttatgaa aaacatcgcc
     2821 gcacaaatgg ttaactttga ctgtgagcag atgcgccgga tcgccaacaa catgccggaa
     2881 cagtacgacg aaaagccaca ggtacagcag gtagcgcaga tcatcaacgg tgtgttcagg
     2941 cagttactgg caactttccc ggcgagcctg gctaaccgtg accagaatga actgaacgaa
     3001 atccgccgcc agtgggttct ggctttccgg gaaaacggga tcaccacaat ggaacaggtt
     3061 aacgcaggaa
//
LOCUS       DABSYT020000237         3051 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.239, whole genome shotgun sequence.
ACCESSION   DABSYT020000237 DABSYT020000000
VERSION     DABSYT020000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.239"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(178..435)
                     /locus_tag="ID725_RS25210"
                     /old_locus_tag="pgaptmp_005042"
     CDS             complement(178..435)
                     /locus_tag="ID725_RS25210"
                     /old_locus_tag="pgaptmp_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853437.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(432..929)
                     /locus_tag="ID725_RS25215"
                     /old_locus_tag="pgaptmp_005043"
     CDS             complement(432..929)
                     /locus_tag="ID725_RS25215"
                     /old_locus_tag="pgaptmp_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGV7853438.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGAHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELHHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPGLIAEINSLANQLQISI
                     PGLPG"
     gene            complement(1132..1569)
                     /locus_tag="ID725_RS25220"
                     /old_locus_tag="pgaptmp_005044"
     CDS             complement(1132..1569)
                     /locus_tag="ID725_RS25220"
                     /old_locus_tag="pgaptmp_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGV7853439.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1566..2063)
                     /gene="rrrD"
                     /locus_tag="ID725_RS25225"
                     /old_locus_tag="pgaptmp_005045"
     CDS             complement(1566..2063)
                     /gene="rrrD"
                     /locus_tag="ID725_RS25225"
                     /old_locus_tag="pgaptmp_005045"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGV7853440.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2063..2278)
                     /locus_tag="ID725_RS25230"
                     /old_locus_tag="pgaptmp_005046"
     CDS             complement(2063..2278)
                     /locus_tag="ID725_RS25230"
                     /old_locus_tag="pgaptmp_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGV7853441.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(2355..2627)
                     /locus_tag="ID725_RS25235"
                     /old_locus_tag="pgaptmp_005047"
     CDS             complement(2355..2627)
                     /locus_tag="ID725_RS25235"
                     /old_locus_tag="pgaptmp_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGV7853442.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            complement(2668..2847)
                     /locus_tag="ID725_RS25240"
                     /old_locus_tag="pgaptmp_005048"
     CDS             complement(2668..2847)
                     /locus_tag="ID725_RS25240"
                     /old_locus_tag="pgaptmp_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGV7853443.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
BASE COUNT          701 a          813 c          715 g          822 t
ORIGIN      
        1 cgcagaagga attccccgtc gggctacggt catggttaat gcggaaatac agcgacgata
       61 cagcgcatga tgtgtcaggc ttgaatacct ttatccgtta aaagggatat cagttaagtt
      121 atcccgtgta gggtataagc cattatcaag cccaccagta gatgggcttt gtaatggcta
      181 cttcgctttt gcttccgctc gcttacgccg gcgctcttct ttcctctcgg cttttgccat
      241 gtccatgaat gcctgcatga tcgagttccg catcatgtag ctaacaaagt gatgattgac
      301 acagccgttg aggcgcagct gctcgccaaa ctcatccacc gaggccaatg cttccatcat
      361 acccttctcg cctttcatga actctgagaa gtcgcgcccc gctctggagg cgcattcaat
      421 gacacgatca ctcatcccgg aagcccgggg atcgaaatct gcaactggtt agccagggag
      481 ttaatttcag cgatcaaacc aggtttcgtg tagcgccatg ccgcgaggcc ttgtccacag
      541 aagctcgcca tgtctttttt ctgatcaaac tcatgacatt tcatgttgag ctgcgcactt
      601 aagctgttcc gatgatgaag ctctccggtg aagtaatcat cgaggacttt ataggctgca
      661 tatttaaatc cggggtttag ccatgctgcg taatcataag caacgaactt tccgccataa
      721 gttccaccgt gcgcaccgcg ttccgtaaaa accacagatt cgtggttttt ctccagctca
      781 gccaagaact ctttggtctg cttgtttcgc aggtagtggt acggagattc agattcactt
      841 ttaccactgg ctttccacat atcagtgagg cagatcatgc catcttcacc gatacggatt
      901 ggttgattga agagggttaa tgatttcata gcgtgtacct actctttgaa atgaaccttt
      961 gccgcacagg aaaccagccc accgaggctc gccagcacta actggtatcc tcaaaggccc
     1021 attccaaagg ggcaggttcg gtgtaaaaaa catgcgttgc ggtacgcatt tattgcaaaa
     1081 agccccgcat cgcgaggctc attaaatgga ctttgtgatt tgcaaaaaaa attatttcag
     1141 gcattgcgtc ctgatgtact cctgcaggta gttaacctgc gcggttatct tgtcgattcc
     1201 acctcggaga cggtaataat tgagttcagc atctgctgta agtcctgggc tttctccatc
     1261 gcccatgctg ctggctccgg tcgttgactt tgcacaggtg gcggagactt gcaggcgctt
     1321 acgcccagca gaaacatcag cacggagact ttcgatagtc gcattagcat cagcaagctc
     1381 ctttgtgtat ctggcgtcaa gttctgctac atcacgttgc cgcttctgca tatcagcgat
     1441 gatggatgtg gctttatcgc gctgctcttt gtaggcgatg gcgttatcac ggtaatgatt
     1501 aacagcccat gacaggcagg cgataatgca gataaccaga gcggagataa taacggttac
     1561 cctgctcatt gttgccccca caaacagact tcacgctcaa tctcgcggcg agtcatcagc
     1621 cctttccatt gcttaccgcc agcgtatgtc cagcgccgta gctgatcaca tgcgcctttg
     1681 atatcgccct ggtttatttt gcgaagaagc gtcgatgttc tgaaattgcc agcgcccacg
     1741 ttgtaaacga acgagtaaag agcgccgcgc gttgtttccg gtatatcgac gttgatgtac
     1801 gggttaattt gtctggcgac cgtggcaagg tctttattca ggagggcttt gcattctgct
     1861 tcggtatacg ttttaccggg catgatgtct tttccggtgt gtccgtgaca tacagtccat
     1921 acgccaacga tatctttgta tggtatgtag ctgacacctt ccagaccatc gttaccacct
     1981 ggaccagtga tgagcacaga cgctatggca acagccccac caccaatagc agctgcaaca
     2041 gccttgcgta atgacggcga cattattcac ctctcgcagc cttacgctta tcttctttaa
     2101 tcttgaaata aagatttgtc agatacgtca gcaggccaaa cagcagactt cccagcacac
     2161 ctattgccac ccactgggac ggagagactt tgtccagcag ctgcagtaac cagtatcccg
     2221 tccccaccgc tgacgtggtg tatgacacac ctgttgtgat tttttccatc tggtacatac
     2281 cccgtctccc gttatccgga agctcacaac atgaaaaagg ccagcagctg tttactgatg
     2341 gccctgactc cccgttacag catcatgacc gattcgggtt gaggttcagt cgcatcggcg
     2401 accggtgatt caggctgaac ttcaccgctc tctgcggtgg tatctcccgc ttcagtcggt
     2461 ggctctgcct gtacaccaag cagctcatcc agaatggcat caacctctgc atcaagacgc
     2521 gcttccaggt tatggcgaag tttctgtttc agtgcgctcc ggacttcttc agagcgcagg
     2581 acttccttca ctgcttcagc agtgaccagg gatgtaattt ctgacatggg attttctcgt
     2641 cgaaaggtgt gattaagaaa gttgccgcta aatgagcggc tcttcgggtt tgcttccggc
     2701 tgactgactg gcgctgattt tctcagcggc ccttttgtca atctgtctgc gccagaagtc
     2761 acgcatggcc cggtatccac ccgaaaggag atacagcaca cagaccaccg tacagaagta
     2821 cagcattaac tggttcagaa atgtcataat ttctttccgt tattgttgac aataagaact
     2881 gttttcattt aaaaaaccag agcacgaaag tatcgttcct ttattttttc tccataggta
     2941 ttaccaccgc cagcgtccat tcctgtcgct ggcggttttt ttatcatgcc gcagtgtctg
     3001 tgctgttcac ttccaccgca atgctgtcaa tcagcaccgg gtaagtcgca t
//
LOCUS       DABSYT020000238         2969 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.240, whole genome shotgun sequence.
ACCESSION   DABSYT020000238 DABSYT020000000
VERSION     DABSYT020000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.240"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..615
                     /locus_tag="ID725_RS25245"
                     /old_locus_tag="pgaptmp_005049"
     CDS             <1..615
                     /locus_tag="ID725_RS25245"
                     /old_locus_tag="pgaptmp_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGV7853444.1"
                     /translation="GQLEAWQENAMFDTGRYRRKKIRYRDEVTGKMITRDNPPIPGKQ
                     SLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHKAWLLWCYSGSICWEYQIAITQWAWN
                     EFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAELFGGEGYEYKELALLVGVTTKNWSK
                     TFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKAAFSKQSVAKVD"
     gene            826..901
                     /locus_tag="ID725_RS25250"
                     /old_locus_tag="pgaptmp_005050"
     tRNA            826..901
                     /locus_tag="ID725_RS25250"
                     /old_locus_tag="pgaptmp_005050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:859..861,aa:Ile,seq:cat)
     gene            909..985
                     /locus_tag="ID725_RS25255"
                     /old_locus_tag="pgaptmp_005051"
     tRNA            909..985
                     /locus_tag="ID725_RS25255"
                     /old_locus_tag="pgaptmp_005051"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:943..945,aa:Arg,seq:tcg)
     gene            999..1075
                     /locus_tag="ID725_RS25260"
                     /old_locus_tag="pgaptmp_005052"
     tRNA            999..1075
                     /locus_tag="ID725_RS25260"
                     /old_locus_tag="pgaptmp_005052"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1033..1035,aa:Arg,seq:tct)
     gene            1249..1677
                     /locus_tag="ID725_RS25265"
                     /old_locus_tag="pgaptmp_005053"
     CDS             1249..1677
                     /locus_tag="ID725_RS25265"
                     /old_locus_tag="pgaptmp_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HGV7853445.1"
                     /translation="MVFLVKKARRAATEIKKFEKRDLAQVVINAAYLVACADGECEAS
                     EKAKIEQVLRNQPALYAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTREA
                     EDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENYL"
     gene            1674..1841
                     /locus_tag="ID725_RS25270"
                     /old_locus_tag="pgaptmp_005054"
     CDS             1674..1841
                     /locus_tag="ID725_RS25270"
                     /old_locus_tag="pgaptmp_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGV7853446.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(2036..2178)
                     /locus_tag="ID725_RS25275"
                     /old_locus_tag="pgaptmp_005055"
                     /pseudo
     CDS             complement(2036..2178)
                     /locus_tag="ID725_RS25275"
                     /old_locus_tag="pgaptmp_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2155..>2969
                     /locus_tag="ID725_RS25280"
                     /old_locus_tag="pgaptmp_005056"
     CDS             2155..>2969
                     /locus_tag="ID725_RS25280"
                     /old_locus_tag="pgaptmp_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGV7853447.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQDLISRTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGG"
BASE COUNT          718 a          685 c          841 g          725 t
ORIGIN      
        1 ggtcagcttg aagcctggca ggagaatgcc atgttcgata cagggcgtta caggcgaaaa
       61 aaaatccggt accgcgatga agtgactgga aaaatgataa cgcgggataa tccaccaatc
      121 ccgggaaagc aatcgctggc gaaggtgacg tcaattcctc tggtcagtcc ggttgagttt
      181 tcgacatcat cgtggcggcg ggctgttctg tctcttgaag aacatcataa agcctggttg
      241 ttgtggtgtt acagcgggag tatttgttgg gaatatcaga tcgcgataac acagtgggcg
      301 tggaatgaat ttaatactca atccggtacc agaaaaattg cagggaaaac gcaggaacgc
      361 ctgaaaaaat taatctggct ggcggcgcag gcagtaaaag cagaactttt tggtggggaa
      421 ggttatgaat acaaggagct ggcattactg gtgggagtga caactaaaaa ctggtccaaa
      481 acatttactc gtcactgggt tgcaatgaaa cacatttttc accgactgga tagtgaggct
      541 ttattgtttg taatgagaac gcgttcaaaa caaaaggcgg cattttcaaa gcaaagtgtt
      601 gcaaaagtag attgaaaggc atatatttca tgcaaatctg atattttgcc gattttgtac
      661 gtgatggcaa aagcaaacaa aacccgccca caagcgggtt tttttgtgcc acttatctcg
      721 gatagacatg gtgaatgcgc tggtggagga agcaagggta atttttaacc aggtgattct
      781 tgaatgcttg caacattgat ttcgtaacgt tattatcctg cgcccggccc tttagctcag
      841 tggtgagagc gagcgactca taatcgccag gccgctggtt caaatccagc aagggccacc
      901 atcacatacc gccattagct catcgggata gaacgccagc cttcgaagct ggtttcgcgg
      961 ggttcgagtc tccgatggcg gtccattatc ggtattctgc gtttttagct cagccggaca
     1021 gagcaattgc cttctaagca atcggtcact ggttcgaacc cagtacaacg caccacactt
     1081 attttccctc gctcgctttt gcggaccttt tttgtatccg caccacgccc ggcgcatacc
     1141 aaccacagag cctttcgggg ggagcttatg gagtggtcag tgtgactttc tctgtgggca
     1201 gatcgctccc gggcgttggc tcacccaccc aaaggaacgt cacgatgttt ggtatttttg
     1261 gtaaaaaaag cccgcagagc ggcaacggaa attaaaaagt ttgaaaaacg cgatctggca
     1321 caggtggtga ttaacgccgc atacctggtg gcctgtgcag atggtgaatg tgaggcatcc
     1381 gagaaagcga agatcgaaca ggtactgcgt aatcagcctg cgctgtacgc gtttacgtca
     1441 gaaattaatg cgattagcgc aaccattatc ggtcagctgg atacgaactt taaaactggt
     1501 cgtcgtgcgg cgttacgtga gatcgaggat gtgaaacacg atacgcgtga agcggaagat
     1561 gtgctggatg tggcggtggc cattgcggag gcagacggcg aaattgagcc ggaagagcgc
     1621 aaggtgctgg aagagattgc cggtgttctg ggtcttcgtc tggagaatta cctgtgacgg
     1681 taaaactgcg cctgactgtg gctgcactcc tgctgtttct ggtggtgatg gtggatttca
     1741 ccagcagaat catgtcggtg ctggcggatg gggtgctggt ctgcggcatt atggtattgc
     1801 tgtggccggt gataaaaaga aacagcctgc ataatgcttg atttttttgt ttgctgttta
     1861 ttaaaaacac ttctgcatgg tgaatccccc tgtgcggtgg ggcaatcagc aagaaggaat
     1921 atggggtaat cgcggattca ggtgctgata ctgaattcac cgggaggcac ccggcaccat
     1981 gctttgccac aaaagtgtta tttctgtttt tctcaaacta tcatcgttat ccctttattt
     2041 tcggctgcgc atggcgcggc ctttttttac gaccagccac tggcagatgg tcatcctgtg
     2101 atttgattcc gcttccggct ttttaactct gttcctctac acgggagaaa ttcgatgtcg
     2161 attaaacatt atgatgttgt cagggcggcg tcgccgtcag accttgcgga aaagctgaca
     2221 cacaaactga aagagggctg gcagccatac ggcggaccgg ttgccattac gccgtacaca
     2281 ctgatgcagg cggtggctat tgaaggagag ccacaggtcg gcccttcatc tgagccggat
     2341 tggtactacg tcatcgtact ggccgggcag tccaatgcca tggcttacgg tgaagggctt
     2401 ccgctgccgg attcatacga tgctccggat ccgcgcatta aacagctggc gcgccgcagt
     2461 acagtgacgc cgggcggggc tgcctgcaga tataacgata ttattccggc tgaccactgt
     2521 ctgcatgatg tgcaggatat gagtacgctg aatcatccga gggctgacct gagcaaaggg
     2581 cagtacggct gtgtcggcca gggtttacat attgccaaaa aactgctccc gtatatcccg
     2641 aataacgcgg ggatcctgct ggtaccatgc tgtcgtggtg gttcggcatt tacccagggc
     2701 gcggagggga cattcagcga gtccacgggg gccagtcagg attcggcacg ctggggggtg
     2761 ggcaagccgt tatatcagga tctgatttcc cgcacaaaag cggcattgca gaaaaatccc
     2821 aaaaacgttc tgctggccgt ctgctggatg cagggtgagt ttgacatgag cgccgccacc
     2881 cacgcacagc aacctgcgct gtttacagcc atgctgacac agtttcgtgc tgacctctcc
     2941 gtgtttaacg cgcagtgcca tggtggcag
//
LOCUS       DABSYT020000239         2907 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.241, whole genome shotgun sequence.
ACCESSION   DABSYT020000239 DABSYT020000000
VERSION     DABSYT020000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.241"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1639)
                     /gene="ehxB"
                     /locus_tag="ID725_RS25285"
                     /old_locus_tag="pgaptmp_005057"
                     /gene_synonym="hlyB"
     CDS             complement(<1..1639)
                     /gene="ehxB"
                     /locus_tag="ID725_RS25285"
                     /old_locus_tag="pgaptmp_005057"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HGV7853448.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIG"
     gene            complement(1689..>2907)
                     /gene="ehxA"
                     /locus_tag="ID725_RS25290"
                     /old_locus_tag="pgaptmp_005058"
     CDS             complement(1689..>2907)
                     /gene="ehxA"
                     /locus_tag="ID725_RS25290"
                     /old_locus_tag="pgaptmp_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HGV7853449.1"
                     /translation="QARIISELGDKDDVVYSGAGSSEVFAGEGYDTVSYNKTDVGKLT
                     IDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQYRDFEFRTGGIPYD
                     VIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGNDRLYGDDGDDYISG
                     GQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSGGTGDDKLYGGGGI
                     DLLDGGEGNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDRLHLSDISFDDIAFKRVG
                     NDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVITDKDGREIKVDKI
                     PHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSGDERLASLYNLSLH
                     QNNTHSTTLTTTV"
BASE COUNT          916 a          668 c          427 g          896 t
ORIGIN      
        1 caccaatctg gcgtcgtaac cattctggat ctgcaagtga taaatcatgc ccatcaatta
       61 aaatctgtcc ggtctctggt atataaaagc gctgaagcag ttttgttaat gtactttttc
      121 ctgaaccaga acggccaact ataccgataa catccccctt tgatatgtat aaattaatat
      181 tattcaaaat aacattaccg tcagaagaat accgaaatct gacattttta aattcgatat
      241 ccccctgaat ttccggcagt atatttctgc ctgacttttt ctctaccggt gtatttaata
      301 catcaccaag gcgctcaaca gaaataccaa cctgctgaaa atcctgccaa agatgagcca
      361 gtctgataac aggagcaatg acctgacctg caagcatatt aaacgcgatt aactgcccga
      421 tgcttatctc tcccgatatg acaagatgtg ctcccatcca tagactaatt accattacac
      481 ttttctgtat cagctgtatc ccctgctgcc ctgtcattgc aaccagattt accctgaaac
      541 tggaagcaac atacccggcc agttgtttat cccatgtagc aatcatctgt ggtgatactg
      601 ccatggattt gattgtattt attgctgtta ccgtttcgac aagaaaagct tgattttctg
      661 cattcctgag aaacttatca tcaagacgtc gacgtaataa gggtgatata aacaatgacc
      721 atataacata acaaggtagt gacaataata taaccagtgt taattgaggg ctgtaatacc
      781 acatgacaca aaaaaatatt acagaaaaaa atagatcaag aactgatgtc aacgcttgtc
      841 cggttaaaaa attacgaatt tgctccagtt cccttactct ggcaacggtc tctccgaccc
      901 tgcgattttc aaaatatgaa acaggcaatg caagcaaatg tctgaataac ttagcaccca
      961 gttcgacgtc aatacgactt gtagtatgag agaaaatata agttctggct ccggttaata
     1021 tcacttcaaa aagtatcact ataataaatg ctattgtgat aatatttaac gttgaaaacc
     1081 cccggtgaac taaaacctta tccattacaa cctgaaaaaa aagaggtgtt attaacgcaa
     1141 gaaactgaat aaaagcagaa acagttaaca cctcaagtaa aatcctcctg tatttcacaa
     1201 cagaggggat aaaccaagaa aaatcaaatt tagctaactc tccgactact gttgctcttg
     1261 acgtaaccag aatgactttt ccctgataca gtttttcaaa ctcatcccga ctaaaagtta
     1321 gtgactgatg ttgttctgga tcataaacaa gatagcgtga tgaatcttta gtaatacgag
     1381 acaatatata atgcttaccg tcatcccgcc atatcaacgc aggtaaagaa attattgaca
     1441 atctggaaaa atgtttttct acatattttg cttttaagcc aatagatttc gctgccagta
     1501 accattcagt caccccaaaa tcttgtgtgt gggtattata ctgatgcctt atagtttcag
     1561 cattgacagt tatattatga tattgtgcaa gcaatatcag tgcatacaga ctattatgag
     1621 aactacattt actcatcata taacgatgac cattcctcct ggaatggcca tcacctcctc
     1681 ttttagtctc agacagttgt cgttaaagtt gttgagtgtg tgttgttttg atgtaaggat
     1741 aaattatata aagatgctaa tcgttcatca cctgaatcga accctgaaac tgaagaaata
     1801 atcttattaa tatcattggc aacactggtg atattaacca tgtttttttc agatgctata
     1861 ttactggctt ttatataacc tgaccgttca ttattattat gaggtatctt atcaactttt
     1921 atctctcgac catctttatc tgttataacc tcaacaagat gattatctgc tcctgaggca
     1981 tctttcgcaa accagttttt aaatgttatc ccattgacat catttgactc attaaatgaa
     2041 agtacaccat taatggcttt attcatgata agatcatttc caactctctt aaatgcgata
     2101 tcatcaaagc taatatcaga taagtgcaaa cgatctcctt tacctccttc atctgcaatt
     2161 gtatgatgac catagttttg cccataaaca taaatatcat taccaaaacc accattcaga
     2221 tagtcattac cttcccctcc atccagaaga tcaataccac caccaccata aagtttatca
     2281 tcaccagtac cacctgacag aatattataa gctccgtgtg cctgaagctc atcattaccg
     2341 cttcctcctg tcagataatt attaccatcc cctccactca atttatcgtt tccactacca
     2401 ccaaataact ggtcgtctcc ctgtcctccg gatatataat catccccatc atcgccgtat
     2461 agtcgatcat taccataatt accttcgata taatcgttcc catctgcgcc atggaatata
     2521 tcattaaact taccgccttt gaattcatca tcatgttttc cgccaatgag ctcttcaaca
     2581 gaatgaagat tatctattac atcataagga attccaccgg ttctgaattc aaaatcacga
     2641 tattgtattt tctctgttcg cttccctact gacacctcct gttccttaac gacttcctgc
     2701 aataccttca cgtcaccata catatttttt gaaactatat actcaccagg ttttgatgct
     2761 cctgttgcat caattgttag tttaccaaca tccgtcttat tataagatac ggtatcataa
     2821 ccttcaccag caaatacttc tgatgagcct gctccagaat aaaccacatc gtctttatct
     2881 ccgagctcag aaattattct cgcctga
//
LOCUS       DABSYT020000240         2888 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.242, whole genome shotgun sequence.
ACCESSION   DABSYT020000240 DABSYT020000000
VERSION     DABSYT020000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.242"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(556..729)
                     /locus_tag="ID725_RS25295"
                     /old_locus_tag="pgaptmp_005059"
     CDS             complement(556..729)
                     /locus_tag="ID725_RS25295"
                     /old_locus_tag="pgaptmp_005059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853450.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            907..1110
                     /locus_tag="ID725_RS25300"
                     /old_locus_tag="pgaptmp_005060"
     CDS             907..1110
                     /locus_tag="ID725_RS25300"
                     /old_locus_tag="pgaptmp_005060"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HGV7853451.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(1042..1515)
                     /locus_tag="ID725_RS25305"
                     /old_locus_tag="pgaptmp_005061"
     CDS             complement(1042..1515)
                     /locus_tag="ID725_RS25305"
                     /old_locus_tag="pgaptmp_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853452.1"
                     /translation="MQVMPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGT
                     FFSLVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRT
                     VTEVNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1435..2175
                     /locus_tag="ID725_RS25310"
                     /old_locus_tag="pgaptmp_005062"
     CDS             1435..2175
                     /locus_tag="ID725_RS25310"
                     /old_locus_tag="pgaptmp_005062"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGV7853453.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            2409..2888
                     /locus_tag="ID725_RS25315"
                     /old_locus_tag="pgaptmp_005063"
                     /pseudo
     CDS             2409..2888
                     /locus_tag="ID725_RS25315"
                     /old_locus_tag="pgaptmp_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          784 a          711 c          659 g          734 t
ORIGIN      
        1 gtttttttca tttaaccctc cttcattaat aaatgatttc tgaaaatcaa aaaggaccac
       61 acatctcaaa aacattattt gaaatcatca tcaaaaaact ataaatatat cacctgacga
      121 tttgaacaca ctgacaaaaa accaagcatg gtaataaacc gaactcccca caaacttctg
      181 ttgcctcaga tactcccccc cctttcctca ggcacaaatg ttgttccata acgaaccagc
      241 cctgttcctt taaactctgc atcaatggca taatgtatac tcaccagact gagtttgctc
      301 ctgcatcatt atttcgatac gttaatgcat tgttcattgc atccggcatt ccccatcgaa
      361 tatttacttt gttatatcag tcagcccata accaggtaaa tttttaaggc ttactgactg
      421 aatattcaaa atgtgttaca tgttatcaaa cctttaaaga ataacctgct cttcttcatt
      481 ggccggattt agctcaatct tgtcagcaaa actaatataa agccgttcat aaagagaagg
      541 atgtagcttc gttatttatc ttttacatgt aacagtgcca ggggatattc cgcacatctc
      601 tgtggctatc atccccacca cgtctccagt ggcagcacct gcggcactgg cacgggcgtt
      661 atttccctgt accaccgcca gtgcagcatt caccacggca tgagctgcca catttgcagt
      721 cttattcacc acacctgtaa cctgtctctt atacacaaat ccccacccgg ggatttgtga
      781 tgtctgtcag tctgctttag ggggactctt tccatccggt agtttccttc ctccccggca
      841 gcgatttttt gaactttttc ttcacccgtt gatcttctct tatatttcat tttcttcggg
      901 tctgttatgg tactttctga ttgttattca ctggctaacg agcagtctgg acatgcccgt
      961 ctcggtgatc cgcgtcgtac acgacgcctt gtgtcactga cctcttctct cgctcagcat
     1021 gcaggtcttt ctattgtgaa atcatcccac ttcactgctc aggttgaagg tgcgtaccgc
     1081 ctgatacgta acccgtcggt gtccccgtaa gccattgccg aagccggatt tactgccact
     1141 gcaagggcat gtgaggcaca tccgctgctc ctcgcgctgg aagataccac caccattaac
     1201 ttcggtcaca gtacggcatt tgatgacctg gggaatacca ccactaaccc gaagacgcac
     1261 gggctgctgg ctcactctgt cctgatgtac gcccccgaca gcgccctgcc agtcgatctt
     1321 atcgaacagc agtgctggag tcgtgccacg gacacctacg gtgttaagca ccagcgaaaa
     1381 gaacgtccct gtgaagaaaa agaaagttac cgctggcagc aggcttcgga gcgcatggcc
     1441 gaacggctga gggagataca gaaacaggta atcactgtct gtgaccggga agcggatatc
     1501 tggcattacc tgcattataa agtcagccgc gggaagccac acgctgaatg tgatgcaaaa
     1561 aggcgggcgg gcagcccgcc aggcccggat gctcatcagt tacagcgaag tcagcataaa
     1621 aaatcccgac aacagcggcc aggcgctctc gctcacgtat gtctgttgcg gggagcagga
     1681 gaggacggag cctgctggta tccgctgaca tcagaaaaag tggcgagtgc cgccgatgcg
     1741 cgacgtatcg tcagccatta cgagcaacgc tggctgatag aggaatacca caaagcgtgg
     1801 aaaagtggtg gtacatgtgt ggaatcgctg cgaatgcaga cccgggataa ccttgagcgc
     1861 atggtggtta tcgcggtgcg ggtgctgggc ctgcggcagg gaggtatcag tgaagaaaca
     1921 caaaatgaca gctgtgagaa aatactgacg ccgacagagt ggaaactact gtgggttaag
     1981 ctggaaggaa agcagcttcc ggcgcaaaca ccgaccctga aatgggcctg tttgaaactg
     2041 gctaaactgg ggcgatggca tgacagcaaa cgcacaagca gccccggttg ggtagttatg
     2101 tgggatggct ggttcagaca tcaggatatg gctgagggtt acctggtgat gaagtctctt
     2161 gatcaggaga tctgatcaag agacaggtca aagatggggg acaacgtctg gtgctgtata
     2221 acagcacagg cctcacactc ttgacgcccg tggaatatat tagagaacaa aaatttcaat
     2281 atgtggtgga cgcatacaga ggattgacag cattcccata ctaattgtta agattcaaat
     2341 tgaagtatat ctatctgtca ttttgcaaat gatatgcggt agctttacct aactatcatg
     2401 aacatttcga aaaatcatcc acaaagcccc gaagaataaa gcactatcgc ccgataaaga
     2461 cctgtaagct cagtggcatg gatactattg agctcagaat ggcgagctgt accgttattt
     2521 cattaccatg ctcgacgaac acagcaacta ctctctggcc gtactatcgc ttaaaagtga
     2581 tatcatcagg catttcttct gctgtgtagc ccagttgttc cttgttggta tcaaccagat
     2641 catcactgat gatgggcaaa gagttctcgg gaaaattcga caaaacgcta caggaagcca
     2701 ccgtcgggtt cgtatggact tatctctata cgccgaaaat aaatgccata agtgagcatt
     2761 ttaaccgaac actgagggaa caatttattg agtttaatga agaattgctc tttgaatagc
     2821 tgaatatgtt caatctgaaa ctggctgagt atctggtact gtacaacagt aaaattcttc
     2881 ataaatcg
//
LOCUS       DABSYT020000241         2822 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.243, whole genome shotgun sequence.
ACCESSION   DABSYT020000241 DABSYT020000000
VERSION     DABSYT020000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.243"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            138..2417
                     /gene="ydeP"
                     /locus_tag="ID725_RS25320"
                     /old_locus_tag="pgaptmp_005064"
     CDS             138..2417
                     /gene="ydeP"
                     /locus_tag="ID725_RS25320"
                     /old_locus_tag="pgaptmp_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HGV7853454.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2665..>2822
                     /gene="safA"
                     /locus_tag="ID725_RS25325"
                     /old_locus_tag="pgaptmp_005065"
     CDS             2665..>2822
                     /gene="safA"
                     /locus_tag="ID725_RS25325"
                     /old_locus_tag="pgaptmp_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HGV7853455.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVILLLTLTLIAIFSAIDQLG
                     ISEMGRIA"
BASE COUNT          760 a          651 c          686 g          725 t
ORIGIN      
        1 taacgataat agattaaaaa tagaaagaga gtagtcccct actcttatat atccatgttg
       61 gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa taattcattt
      121 ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggtgct gccggtggtt
      181 ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata cgccaggatg
      241 ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt tgtgcatggc
      301 cagatcctaa gcacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa gcaatcgcct
      361 gggaagtcac ggataagcag gtaaatgcct ctttctttgc tcagaatacg gttcaatcat
      421 tacttacctg gggagatcac gagcttgagg ctgcgggacg tctcactcag cctttgaaat
      481 atgatgctgt cagcgactgt tacaagccat taagctggca acaagctttc gacgaaattg
      541 gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact tcgggccgca
      601 cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg agcaataact
      661 ttcccgactg ctccaacatg tgccatgaac cgacaagcgt gggtttggca gcgagtatcg
      721 gtgtaggtaa agggaccgtg ttgctggaag actttgagaa gtgcgattta gtcatttgca
      781 ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc gctttagtga
      841 aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg gagcgattta
      901 ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg gccagtgcct
      961 actataacgt gcgcattggt ggtgatatgg cgttgctcaa ggggatgatg cgcctgttaa
     1021 ttgagcgcga tgatgctgca agcgccgcag gtcggccctc attgctggat gacgaattta
     1081 ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgtgctcaat tccgagtgga
     1141 aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactg gctgatgcat
     1201 atgccgctgc cgaacgaacc attatctgtt acggaatggg gatcactcag cacgaacatg
     1261 gtacccagaa tgtacagcaa ctggtcaatc tgctgttgat gaaaggtaac attggcaagc
     1321 ctggtgcggg tatctgccca ctacgtggcc actctaatgt acagggcgac cgaaccgtcg
     1381 gtatcaccga gaaaccgtct gtagagtttc tggctcgcct gggtgagcgc tatggcttca
     1441 ccccacctca tgtacctgga catgctgcaa ttgccagcat gcaagcaata tgtacggggc
     1501 aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca gatcgggaag
     1561 cgagcgctgt accgttaacg caattagatt tggcggtaca cgtagccact aagcttaacc
     1621 gctctcatct gctgaccgca cggcatagct atattctgcc ggttctggga cgtagcgaga
     1681 ttgacatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg tcgatgattc
     1741 atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag tgtgctgtgg
     1801 tcgcgggaat cgcgcaggca acactacccc agagcgtggt agcctgggag tatctggtgg
     1861 aagattatga tcgcattcgc aatgacattg aagctgtgct gccagagttc gccgactata
     1921 accaacgcat ccgtcatccc ggtggttttc acctgataaa tgcagctgct gaaaggcgct
     1981 ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa agggctgtta gaagatccct
     2041 cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagccacgat cagtacaaca
     2101 cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga gatgtggtct
     2161 ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga gttaatctta
     2221 ttgcgcttac gccagacggt aagcgcagct cacgccgcat ggatagatta aaagtggtca
     2281 tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat cacatgctaa
     2341 cacttgataa ccacgatcca ttaagtggca ttcctggcta taaaagtatt ccggttgaat
     2401 tagaaccatc aaattaatgc ctcttctcat ttcttctgct gtcatccgca cagcagaaga
     2461 attcctcatt gattattatc gcgcaatttg ctcacatgga ttaaattaaa ctacatacta
     2521 taagatataa acttctgcct acagctgtaa gaaactccgc tcagtactga agcaccagtc
     2581 ctatttcctc ttttctcaag cctgttatat taagcatact gattaacgat ttttaacgtt
     2641 atccgctaaa taaacatatt tgaaatgcat gcgaccacag tgaaaaacaa aatcacgcaa
     2701 agagacaact ataaagaaat catgtctgta attgtggtta tcttattact gacccttacg
     2761 ttgatagcca ttttttcggc aattgatcag ctgggtattt cagaaatggg tcgcattgca
     2821 ag
//
LOCUS       DABSYT020000242         2796 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.244, whole genome shotgun sequence.
ACCESSION   DABSYT020000242 DABSYT020000000
VERSION     DABSYT020000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.244"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..684
                     /locus_tag="ID725_RS25330"
                     /old_locus_tag="pgaptmp_005066"
     CDS             <1..684
                     /locus_tag="ID725_RS25330"
                     /old_locus_tag="pgaptmp_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853456.1"
                     /translation="SAAAQKASESSQSAADAELSKKTAESAAGNAARDATTATEKARE
                     SAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGDTGPAGA
                     TGERGPAGDAGPAGPAGPAGPQGPKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPG
                     NIIETNSHGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRFGDGPWQDVKGLDEV
                     GSDTGRTGE"
     gene            686..955
                     /locus_tag="ID725_RS25335"
                     /old_locus_tag="pgaptmp_005067"
     CDS             686..955
                     /locus_tag="ID725_RS25335"
                     /old_locus_tag="pgaptmp_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853457.1"
                     /translation="MNILKKIMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            1069..1644
                     /locus_tag="ID725_RS25340"
                     /old_locus_tag="pgaptmp_005068"
     CDS             1069..1644
                     /locus_tag="ID725_RS25340"
                     /old_locus_tag="pgaptmp_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGV7853458.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
     gene            2355..>2796
                     /gene="nleG7"
                     /locus_tag="ID725_RS25345"
                     /old_locus_tag="pgaptmp_005069"
     CDS             2355..>2796
                     /gene="nleG7"
                     /locus_tag="ID725_RS25345"
                     /old_locus_tag="pgaptmp_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HGV7853459.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGV"
BASE COUNT          815 a          550 c          735 g          696 t
ORIGIN      
        1 tctgcggccg ctcaaaaagc cagtgagtca tcacaaagtg cagcagatgc tgagttgtca
       61 aaaaagacgg cagaaagtgc agccggtaat gcagccaggg atgcaacgac cgcaacagaa
      121 aaagcccggg agtcagcaga aagcgcacag tcagcggaac aaagcaggat agcggcggaa
      181 gaggccgtaa accgaatccc cacggtggtg gggcctcccg ggccaaaggg ggaaccgggt
      241 cccgcgggtc ctcaggggcc gaagggagat aaaggagagc gtggagacac cggtccggca
      301 ggggcaaccg gtgaaagggg gccggcaggt gatgctggtc cggcaggccc ggcaggcccg
      361 gcaggcccac agggaccgaa aggagaaaca ggtgcggctg gcccggtggg ggcaaccgga
      421 cctcaggggc cgaagggcga cccgggggag acgcaaatac ggttccgtct ggggccggga
      481 aacattattg agacaaacag ccatggctgg ttcccggata cagatggcgc actcatcacc
      541 ggactgacct ttcttgaccc caaagatgcc acacgggttc agggtttttt tcagcatttg
      601 caggtcaggt ttggtgacgg gccgtggcag gatgttaagg ggctggatga agtgggcagt
      661 gatacaggca gaacaggaga atgacatgaa catattaaaa aaaattatgc agcgtctgtg
      721 cggttgcgga aagcatgatg accgtgaaaa cggggagtta cttacagcac agctgcgact
      781 gggaccggca gacattctgg agtccgatga gaatggtatt atcccggagc aggacagggt
      841 aatcacgcag gtggtgatac tggatgcgga taaaaagcag atacagtgcg tggtaagacc
      901 gctgcaaatc ctgcgtgctg acgggacgtg ggaaaatatt ggcgggatga aatagcccga
      961 cagcttcaca aaaaccggag tccggctccg gtttttgttg tcatgtatgg gggctgtttg
     1021 ttatgactcc ctgtgtttgg aatgaatatt taaataggga gttttgtcat gccgttaaca
     1081 tcagctattg catccaattc attttccacc ggaatgcagg ttcttcgtgc tcaaatggcc
     1141 gccagtggcg gtggagaaat tacagtaggc gggcagacgg tccgtatcac atatagtgaa
     1201 acggatggtc gctttctggc gagtgggggc aataacagtt tgctttctgg attattactt
     1261 acagggctta atggtggtcc tgaagcgctc agggatataa tgttaagaat ggtttcaggt
     1321 tcaggtaaca cacaatcaca tggtgatatt gaggggaaaa tatcacaatg taagttttct
     1381 gttaatacgg agagccttca gtgtccatcc gaggcggttc gatgcccaat tatactggat
     1441 aaaccagaag aaggtgtgtt tgttaaaaat tcagaaggtt ctttggtttg taccttattt
     1501 gattcggttt ctttttctca tttggttcgc gacggtggga agcacccgct aacacgagaa
     1561 ccaataacgt catcaatgat tgtaagtcaa gaacaatgta tttatgacca aaccaaagga
     1621 aactttgtca taaaggataa gtgaaataaa tattacccaa gctatatgtt aactgccagt
     1681 tgcttatatg aaatgctaca gatgttcagg gtataaggat gtggtaattg gtgtactgga
     1741 tgcagctaaa aagcatatac agtgagtggt gaggccgctg caaatctggc gtgctgacgg
     1801 ggaaatattg gcgggatgaa atagccgacg gattcacaaa aaccggagtc cggctccggt
     1861 ttttgttgtc atgtcaggga gatgtttgtt atgaagccca gaggaaatat ttatctgtat
     1921 gaaggaatat ggtaatgcct ggattagtat catatatatc atcgacttca ttcgcgaatg
     1981 agatggcgga gatgcgtcag caggtaatgg aagggcagat tggtggattt ctcctgggag
     2041 gggagagagt tagagtttct tatttatttc aattgcatta atatcttgaa tggattacat
     2101 agagttaatg tatccagtac tccctattct cttgcatata atacatgttg caacttacat
     2161 ctcagcgcta tgaaaaaaca ccacctctct ctttatgaaa ttctggattt accaagcgct
     2221 aatttatcat ttcaatctac ctttaagcat tgcatttatc tccctacgag gtcatacttt
     2281 aggaagttaa atatgaatga taatatacct acagcgcgaa atcacaaaca atcgacttgt
     2341 attacagaaa aaacatgcct atctttttaa acttcacagc aggcagtatc ttacctgaga
     2401 atgagctagc atctttacgt tatattgtgc agcaaaatca aaatgatact gtaatcataa
     2461 aagaacgtta taaaatggat atccgttata tcgaatcagt caatggtttt acagtaaatc
     2521 ctgtatgcag taatcatttc tccatattta tggcgagaca aaacactatt gctcgcaacc
     2581 tggaacagca gatcaacaac ggacgaagtt ttgcacaaat atctcaggat tttatgcttc
     2641 aattatcttc aaatatagga tggaaaaaag gggccgaaaa cgcccttaaa aataaaatcc
     2701 attctcattc atttgttgta aatcctgatg aattctcttg cgacacacaa tttcttaagt
     2761 gcccaataac attatgcgtt ccagaaaaag gggttt
//
LOCUS       DABSYT020000243         2757 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.245, whole genome shotgun sequence.
ACCESSION   DABSYT020000243 DABSYT020000000
VERSION     DABSYT020000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.245"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1661)
                     /locus_tag="ID725_RS25350"
                     /old_locus_tag="pgaptmp_005070"
     CDS             complement(<1..1661)
                     /locus_tag="ID725_RS25350"
                     /old_locus_tag="pgaptmp_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGV7853460.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNPL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL"
     gene            complement(1636..2181)
                     /locus_tag="ID725_RS25355"
                     /old_locus_tag="pgaptmp_005071"
     CDS             complement(1636..2181)
                     /locus_tag="ID725_RS25355"
                     /old_locus_tag="pgaptmp_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGV7853461.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            2568..>2757
                     /locus_tag="ID725_RS25360"
                     /old_locus_tag="pgaptmp_005072"
     CDS             2568..>2757
                     /locus_tag="ID725_RS25360"
                     /old_locus_tag="pgaptmp_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGV7853462.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLINEKYSQFVPNKDK"
BASE COUNT          693 a          757 c          637 g          670 t
ORIGIN      
        1 agatcaaaaa tatccgggtt attcgggaag tgaacggcac cgggaagcgg ttcatcccct
       61 tccggcgtca gtgtgaagcg gttataaatc tgctctttcg cggtatccgt accgatttcg
      121 gtaaggtaaa ccccgttttt gtttcgctta cgtggcatgc tggccaccgg ctttccgtag
      181 acggatgccc ctttaatggg gatcacccgg aacagcccat gttttttcga gcgttcatac
      241 acaatggtcg ggtcaatccc gccagtatcc cagcagatac gggataccga catttctgca
      301 ccattccggc gggtataggt tttattgatg gcctcatcca cacgcagcag cgtctgttca
      361 tcgtcgtggc ggcccataat aatctgccgg tcaatcagcc agctttcctc acccggcccc
      421 catccccata cgcgcatttc gtagcggtcc agctgggagt cgataccggc ggtcaggtaa
      481 gccacacgat caggaacggg cgctgaataa tactctttcc gctctgccat cacttcagca
      541 tccggacgtt cgccgatttt cgcttcccac gtctcaccga gcgtggtgtt cacgaaggtt
      601 ttacgttttc ccgtatcccc tttcgtcttc atccagtctt tgacaatctg cacccaggtg
      661 gtgaacgggc tgtacgccgt ccagatgtga aaggtcacac tgtcaggcgg ttcaatctct
      721 tcaccggatg acgaaaacca gagaatgcca tcacgggtcc agatcccggt cttttcgcag
      781 atataacggg catcagtaaa gtccagctcc tgctggcgga tgacgcaggc attatgctcg
      841 cagagataaa acacgctgga ggggtcatcc ggcgtccatt tgaggccaaa cggcgtctct
      901 ttgtcgccaa atttaagata ctgctcctcc ccgcagtgcg ggcaggcaac atgaaaacgc
      961 ataaaatgcg gggattcact ggctgcacgc tcaatctggc aggtgcctct cactttgggc
     1021 gtggagccac ggatggactt tggccagacc gagccttcaa tacgcttgtc acccaggaac
     1081 gtcggagagc cttcctgttc aatatcctca tcaaaggcag caagttcatc ataacccgcc
     1141 acatcaaccg acttttcacg gtagtttttt gccgctttac cgcccaggca ccagaagcca
     1201 cgaccattgg tgaaacgctt catagtgagc gtgttatccc ggtgcttttt gccataccac
     1261 ggggccagag acagcagcga cggaatatcg cggatggtag gctcgacgtg ggttttcata
     1321 aagttctcgg catcaccatc cgtcggcaac cagataaggg ggttacgctg cttatgctct
     1381 atgaagtagg cataaacacc cagcagcatt tttgaataac caacacgggc agacttcacc
     1441 acattcacct cgcggatgta gtcgctgccc atcgcattca tgatagcccg ctgaaagggt
     1501 agtgtttccc agcgcccttc ctggtatgcg gattctttcg ggagatagta actggcatcc
     1561 gcccattcaa cggcagtctg tggctccggc ctgaacaggg ctcgcagccc ggcgcgtaca
     1621 tcacgccgca gaatatcaat ctgactgttc gatatattca ctcagcaacc ccggtatcag
     1681 ttcatccagc gcggctgctt tgttcatggc tttgataata tcccgtttca ggaaatcaac
     1741 atgtcggttt tccagttccg gaaaacgccg ctgtactgag agagggatcc cgtcaagaat
     1801 actggcaatt tcacctgcga tccgtgacag cacgaaagta cagaatgcgg tttccaccac
     1861 ttctgcggag tctctggcat tcttcagctc ctgggcgtca gcctgcgcac gcgtaagtcg
     1921 atggcgttcg tactcaatag tcccaggctg gagatctgcc tcgctggcag ccctgtaatc
     1981 ctcaacctct ttacggagtt tttcattttc gatatcagct tccctctgcg cataccactg
     2041 aattgccgtg gcggtatcaa atacagattc aacgccctta ccacctccgg agacgcaagg
     2101 gagcccctga gactgccagc gttcaatcgt tcgcggatcc acgttgaaaa tttcggcaag
     2161 tttcttttta ttaaccttca tgaaacagtc tcacaacaaa tacagggtcc gacatgaaag
     2221 tgcccgaaaa tgactttttc aggcgttttc atgtcggacc tttacggatt cgatattaga
     2281 aaaaacaaat agttatgttc gagaagtacc gacatgattt tccccggaaa attttcataa
     2341 atagcgaaaa cccgcgaggt cgccgccccg taaccggtcg gatcgccgga aaggacccgc
     2401 aaaatgataa taattatcat ctacatgtca caacgtgcat ctacgccatc aaaccacgtc
     2461 aaataatcaa ttatgacgca ggtatcgtat taattgatct gcatcaaatt aacgtaaaag
     2521 caacttcaga taatacaaat cagcaacact gaatatgggg caacattatg tcatcaaaga
     2581 acagaacccg cagaacaaca acccgcaaca tccgatttcc aaaccagatg attgaacaaa
     2641 ttaacatcgc tcttgatctg aaaggttcag gaaatttttc agcgtgggtt attgaagcct
     2701 gcagaagaag attaattaat gaaaaatatt ctcaatttgt acccaacaaa gacaaac
//
LOCUS       DABSYT020000244         2741 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.246, whole genome shotgun sequence.
ACCESSION   DABSYT020000244 DABSYT020000000
VERSION     DABSYT020000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.246"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..486
                     /locus_tag="ID725_RS25365"
                     /old_locus_tag="pgaptmp_005073"
     CDS             <1..486
                     /locus_tag="ID725_RS25365"
                     /old_locus_tag="pgaptmp_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HGV7853463.1"
                     /translation="TYDIYQAGLTIYRMCVGHDNFERERSAFSTIEQLRESIINGCYP
                     LKEYPPHIHKKLITIVNKCIHVDPNERYQSVLDVLNDLSAISDGVLDWRLQMTKPTNG
                     TCEWQKKSGDAILSIVFDAENSSTTGFRLYDDGRKRRATNLTISSGCTPTKLYRLLKD
                     N"
     gene            489..653
                     /locus_tag="ID725_RS25370"
                     /old_locus_tag="pgaptmp_005074"
     CDS             489..653
                     /locus_tag="ID725_RS25370"
                     /old_locus_tag="pgaptmp_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853464.1"
                     /translation="MKKREEVSKLPRRRDAALAVPYKKDEFISPSDDRKFSKAKSFTS
                     TSLKDKYFKI"
     gene            1457..1525
                     /locus_tag="ID725_RS25375"
                     /old_locus_tag="pgaptmp_005075"
                     /pseudo
     CDS             1457..1525
                     /locus_tag="ID725_RS25375"
                     /old_locus_tag="pgaptmp_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            1584..2054
                     /locus_tag="ID725_RS25380"
                     /old_locus_tag="pgaptmp_005076"
     CDS             1584..2054
                     /locus_tag="ID725_RS25380"
                     /old_locus_tag="pgaptmp_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853465.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            2205..2573
                     /locus_tag="ID725_RS25385"
                     /old_locus_tag="pgaptmp_005077"
     CDS             2205..2573
                     /locus_tag="ID725_RS25385"
                     /old_locus_tag="pgaptmp_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HGV7853466.1"
                     /translation="MSNINKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPSMDGSEGIR
                     ITDIDTSGIFNSDDMTIKAA"
     gene            2646..>2741
                     /locus_tag="ID725_RS25390"
                     /old_locus_tag="pgaptmp_005078"
     CDS             2646..>2741
                     /locus_tag="ID725_RS25390"
                     /old_locus_tag="pgaptmp_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGV7853467.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKR"
BASE COUNT          910 a          541 c          587 g          703 t
ORIGIN      
        1 acatatgaca tatatcaggc aggattaacc atatatagaa tgtgtgttgg acatgataat
       61 tttgaaagag aaagatctgc atttagcacg attgaacaac tcagagagtc gataattaat
      121 ggctgctatc cattaaaaga gtatcctccc catatacata aaaaattaat aacaatagtg
      181 aacaaatgca ttcatgtaga tccaaatgaa agatatcaat ccgtactaga tgtactaaac
      241 gatctctcag ctataagtga tggcgttctt gactggcgtc tacagatgac gaaaccaact
      301 aacggcacat gcgaatggca aaaaaagtct ggggacgcta tactgtctat agtttttgac
      361 gcagaaaatt cgtctactac tggttttcgt ttatacgatg atgggcggaa aaggcgtgct
      421 acgaacttaa caatatcctc aggatgtacc cctacaaaac tgtataggtt attaaaggat
      481 aactgatcat gaaaaagcgc gaggaagtaa gcaagctgcc tcgcagacgt gatgcagcat
      541 tagcggttcc ctacaaaaaa gatgagttca taagcccttc tgatgacaga aaattttcaa
      601 aggcgaaaag ttttacatct acatctctaa aagataaata ctttaaaatc tagcccggcc
      661 tcagcgccgg gttttctttg cctcacgttc gcccacctaa aaaacataac caattgtatt
      721 tattgatgta actcgctaaa ccatgcagtt atgatccctg ccgcataacc ttcatcagcc
      781 acattttcaa aaataaattt ccttatatat cagaatcata cttcgtagag ttaataaatc
      841 accaaaattc gtaccaatag ttcttgataa tgtcgaacta ttggttcatc attatcatcg
      901 tcagcaggac gcattactca ccagggcggt gaatatacaa cgattcgaat atgaatctac
      961 ggcgctgaca aagcgcaata accaaagtga actttggggt gtggtgaagg gttcatggac
     1021 gggaatatgt cgcacgtaaa gcggcgaggc ctgcgggact attgccgaat tgaagtaggc
     1081 cgaaacaggt cgaaatgggt ctcccaccta ccacaccacc aaagttcatc aggaggtcta
     1141 tatgacacgc agaactcagt tcaaaggcaa ttcacgttct cgtcgtcgtg agcgtttaaa
     1201 ggcaaaggca ttagctaacg gcgtactggc ccgcgaagaa gcaataagtt cagaagtatt
     1261 acaccgccct actctaagca gagcgcagat tcaggctaaa ggtactcacg aaacgcctga
     1321 gcgcatagaa gacgctaagc caattaagtt catggcacag gacgtgatct ggcaacagaa
     1381 agaatacaga cgcaatctgg agcgagcggc cattgtgtac gcgaatgagt ttggacataa
     1441 gcaaccagaa actggtgtat gtcttccaaa cgtagccatt tacgcggcag gctaccggaa
     1501 atcaaaacaa ctgacggcga ggtgacttgt gttggtcgcc agaaaatgaa attaggcagc
     1561 aaaccactta tttgaggtga gatatgacaa aatcatggag cgtacctttt cctgaatcag
     1621 aaactgaaca tgatggaatg cctgttttct ggagattcca ggcgacagtt gaagaagatg
     1681 ggataaaaat attcgcactt caatatatag cttttcatca gacagagcat tatgcatggt
     1741 tggttcctgc gcattggatt gttaatttta aaccagcacc aaatcagtgg ttacaggaat
     1801 ggaaacaaag gagaaataga tatgcaatta agaaagtagc aaaaaatgca gaaagatctt
     1861 ttgcattccc aacgaagaaa cttgccattg aaagtttatt gcgccggaag aaataccatt
     1921 taatgagaat caaacaagat ttggctgttg tatcaactct tgttgatggt atgaagaata
     1981 ttgatacatc aacaccagat attgaatata actttggaca caaccaagaa acagaaaatt
     2041 gggtatttta ttagtacgaa taagcactgt gtattcattc caacgagtga atacacggag
     2101 caatgtcgct cgtaactaaa caggagccga cttgttctga ttattggaaa tcttctttgc
     2161 cctccaatgt gagggcgatt ttttatctgt gaggatatga acagatgtca aacatcaata
     2221 aatacatcat tgattacgac tggaaagcat caatagaaat tgaaatcgac catgacgtaa
     2281 tgacagagga aaaacttcac cagattaata atttctggtc agactctgaa taccgactca
     2341 ataaacacgg ctctgtatta aatgctgtat taatcatgct ggcgcaacat gctctgctta
     2401 tagcaatttc aagcgactta aatgcatatg gtgttgtgtg tgagttcgac tggaatgatg
     2461 gaaatggtca ggaaggatgg ccttcaatgg atggtagcga aggaataaga attaccgata
     2521 tcgatacatc aggaatattt aattcagatg atatgactat caaggccgcc tgagtgcggc
     2581 tttaccgcat accaataacg cttcactcga ggcgtttttc gttatgtata aataaggagc
     2641 acaccatgca atatgccatt gcagggtggc ctgttgctgg ctgcccttcc gaatctttac
     2701 ttgaacgaat cacccgtaaa ttacgtgacg gatggaaacg c
//
LOCUS       DABSYT020000245         2634 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.247, whole genome shotgun sequence.
ACCESSION   DABSYT020000245 DABSYT020000000
VERSION     DABSYT020000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.247"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            116..1282
                     /gene="nhaA"
                     /locus_tag="ID725_RS25395"
                     /old_locus_tag="pgaptmp_005079"
     CDS             116..1282
                     /gene="nhaA"
                     /locus_tag="ID725_RS25395"
                     /old_locus_tag="pgaptmp_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGV7853468.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1348..2247
                     /gene="nhaR"
                     /locus_tag="ID725_RS25400"
                     /old_locus_tag="pgaptmp_005080"
     CDS             1348..2247
                     /gene="nhaR"
                     /locus_tag="ID725_RS25400"
                     /old_locus_tag="pgaptmp_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGV7853469.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2285..>2634)
                     /locus_tag="ID725_RS25405"
                     /old_locus_tag="pgaptmp_005081"
     CDS             complement(2285..>2634)
                     /locus_tag="ID725_RS25405"
                     /old_locus_tag="pgaptmp_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGV7853470.1"
                     /translation="FHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNEL
                     ENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSG
                     QIMSRVKIVVMYN"
BASE COUNT          606 a          575 c          681 g          772 t
ORIGIN      
        1 ttcttctata gatcgtaaaa aacgaattat tcctacacta taatctgatt ttaacgatga
       61 ttcgtgcggg gtaaaatagt aaaaacgatc tattcacctg aaagagaaat aaaaagtgaa
      121 acatctgcat cgattcttta gcagtgatgc ctcgggaggc attattctta tcattgccgc
      181 tatcctggcg atgatgatgg ccaacagcgg cgcaaccagt ggatggtatc acgactttct
      241 ggagacgccg gttcagctcc gggttggttc actcgaaatc aacaaaaaca tgctgttatg
      301 gataaatgac gcgctgatgg cggtattttt cctgttagtc ggtctggaag ttaaacgtga
      361 actgatgcaa ggatcgctag ccagcttacg ccaggccgca tttccagtta tcgctgctat
      421 tggtgggatg attgtgccgg cattactcta tctggccttt aactatgccg atccgattac
      481 ccgcgaaggg tgggcgatcc cggcggctac tgacattgcc tttgcacttg gtgtgctggc
      541 gctattggga agtcgtgttc cgttagcgct gaagatcttt ttgatggctc tggctattat
      601 cgacgatctt ggggccatca ttatcatcgc attgttctac actaatgact tatcgatggc
      661 ctctcttggc gtcgcggctg tagcaattgc tgtactcgcg gtattgaatc tgtgtggtgt
      721 acgccgcacg ggcgtctata ttctggttgg cgtagtgttg tggactgcag tgttgaaatc
      781 gggggttcac gcaaccctgg cgggggtaat tgtcggcttc tttattcctt tgaaagagaa
      841 gcatgggcgt tctccagcga agcgactgga gcatgtgttg cacccgtggg tggcgtatct
      901 gattttacca ctgtttgcat ttgctaatgc tggcgtttca ctgcaaggcg tcacgctgga
      961 tggcttgacc tccattctgc cattggggat catcgctggc ttgctgattg gcaaaccgct
     1021 ggggattagt ctgttctgct ggttggcgct gcgtttgaaa ctggcgcatc tgcctgaggg
     1081 aacgacttat cagcaaatta tggcggtggg gatcctgtgc ggtatcggtt ttactatgtc
     1141 tatctttatt gccagcctgg cctttggtag cgtagatcca gaactgatta actgggcgaa
     1201 actcggtatc ctggtcggtt ctatctcttc ggcggtaatt ggatacagct ggttacgcgt
     1261 tcgtttgcgt ccatcagttt gacaggacgg tttaccgggg agccataaac ggctcccttt
     1321 tcattgttat cagggagaga aatgagcatg tctcatatca attacaacca cttgtattac
     1381 ttctggcatg tttataaaga aggttctgtg gttggcgcag cggaggcgct ttatttaaca
     1441 ccacaaacca ttaccggaca gattcgggcg ctggaagagc gcctgcaagg caaattattt
     1501 aagcgcaagg gacgtggtct cgagcccagc gagctggggg agctggtcta tcgctatgcc
     1561 gataaaatgt ttaccttgag ccaggaaatg ctggatatcg tgaactatcg caaagaatcc
     1621 aatttattgt ttgacgttgg cgtggctgat gtactttcca aacgtctggt cagtagcgtg
     1681 ctgaatgctg cagtagtaga aggcgagccg attcatcttc gctgcttcga atccacccac
     1741 gaaatgctgc tggagcaatt aagccagcat aaactggata tgatcatttc cgactgtccg
     1801 atagattcta cgcagcagga aggcctgttc tccgtgagaa ttggcgaatg tggcgtgagt
     1861 ttctggtgta caaatccacc gccggaaaaa ccgttcccag cttgtcttga agaacggcgg
     1921 cttttgattc ctgggcgacg ttcaatgtta gggcgcaaat tacttaactg gtttaactcc
     1981 cagggattaa acgtagaaat ccttggcgag tttgatgatg ccgctttgat gaaagctttt
     2041 ggtgcgatgc acaatgcaat cttcgttgcc ccaacgcttt atgcatatga cttttatgcc
     2101 gataaaactg tcgtagaaat tggtcgcgtc gagaatgtga tggaagagta ccatgctatt
     2161 tttgctgagc ggatgattca gcacccggcg gtacagcgaa tctgcaatac ggattattct
     2221 gcgcttttta gtccagcggt gcgttaatcg gcagctcccc caaagttaag gtgggggaga
     2281 tagattagtt gtacattacc acgattttga ctcggctcat tatttgcccg cttgagacat
     2341 tgtttccata tgtacgcggg cgaataaata gaggaatctg attacttcct tcatggggat
     2401 gctgaaaaga gtagtaattg cttttaccat actggatttc attcgtacca tcattggaga
     2461 gttgaaagcc tatttgaccc gcaccgtcct cactgttttc taactcattt tttaatatgc
     2521 tggttcctga ctcctgtatc gtcactggtt caaagtaata ttcaacgtca ctgggttcaa
     2581 aaactaattc accttgctcg ccctggatgc agtcaaatct tatatgatga aact
//
LOCUS       DABSYT020000246         2633 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.248, whole genome shotgun sequence.
ACCESSION   DABSYT020000246 DABSYT020000000
VERSION     DABSYT020000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.248"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..566
                     /locus_tag="ID725_RS25410"
                     /old_locus_tag="pgaptmp_005082"
     CDS             <1..566
                     /locus_tag="ID725_RS25410"
                     /old_locus_tag="pgaptmp_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGV7853471.1"
                     /translation="AVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQYFK
                     LQLLDASNPRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQAIE
                     TLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNLKN
                     QDTNNSSFSYGDNRFEKSQGSKWTFQE"
     gene            580..819
                     /locus_tag="ID725_RS25415"
                     /old_locus_tag="pgaptmp_005083"
     CDS             580..819
                     /locus_tag="ID725_RS25415"
                     /old_locus_tag="pgaptmp_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HGV7853472.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            839..1171
                     /gene="sctI"
                     /locus_tag="ID725_RS25420"
                     /old_locus_tag="pgaptmp_005084"
     CDS             839..1171
                     /gene="sctI"
                     /locus_tag="ID725_RS25420"
                     /old_locus_tag="pgaptmp_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HGV7853473.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            1168..1902
                     /locus_tag="ID725_RS25425"
                     /old_locus_tag="pgaptmp_005085"
     CDS             1168..1902
                     /locus_tag="ID725_RS25425"
                     /old_locus_tag="pgaptmp_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HGV7853474.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            1918..2499
                     /locus_tag="ID725_RS25430"
                     /old_locus_tag="pgaptmp_005086"
     CDS             1918..2499
                     /locus_tag="ID725_RS25430"
                     /old_locus_tag="pgaptmp_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HGV7853475.1"
                     /translation="MNLALRKIIYAPISYIHPQRVSLNNTPINNPVLRSITNEMILLQ
                     YNLSVEHFNLNSSLIYYINNWNLLPLICLLSGCHFYRERFAERGFFYKVPDVLRDYLS
                     AIPLEINEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHILS
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            2441..>2633
                     /locus_tag="ID725_RS25435"
                     /old_locus_tag="pgaptmp_005087"
     CDS             2441..>2633
                     /locus_tag="ID725_RS25435"
                     /old_locus_tag="pgaptmp_005087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HGV7853476.1"
                     /translation="MRKKIEMSLIKSPANGVVIKRKISDGLKEIVSLKEKILLETTAK
                     IQSIEEKREEKFIQGYYDGY"
BASE COUNT          903 a          440 c          498 g          792 t
ORIGIN      
        1 aagcagtctg ggtcaaacaa gcactggaaa aaaatcaacc aggaaagcca gtacgggtga
       61 taaatcctga tgatgaatcg atacgaattt tttcatggct tgctgataat ttccctgatt
      121 tacaatattt taaacttcag ctattagatg ccagtaatcc cagactgacc gtgagtaagc
      181 aacggaatgc tatcacacag caactaatcg acaatcttat taaagggtta ctacaaacta
      241 tgccatatgc cagcaatata agtattgcgg tattagatga taatgtattg gaaagtcagg
      301 ctattgaaac attgtctgca ataggtcttt cttatgaaaa atataaaaca gctaacaatg
      361 tgtatttcaa tatcattggt acgttaagcg acagtgaatt aaacaaaata aataactatg
      421 ttgacgaata ttataaacaa tggggtaaac aatatgtaag atttaatgtg aatttgaaaa
      481 atcaggacac aaataatagt tcatttagct acggagataa ccgattcgag aagtctcaag
      541 gtagcaagtg gacatttcag gaataattga ggaggactta tggcagattg gaatggttat
      601 attatggaca tcagcaaaca atttgatcag ggcgttgatg atctgaacca gcaagttgaa
      661 aaagcgttgg aggatttagc aaccaatcct tccgacccga aattccttgc cgaatatcaa
      721 agtgcattag ctgagtatac attatatcga aatgcgcaat ctaacgttgt aaaagcgtat
      781 aaagatcttg attcggcaat catacaaaac ttccgctaaa ctggataagg attttattat
      841 gtcagtaagc aatatgccac ctattgatag agctgagcaa tcaactgctc atgaaataca
      901 gcaagcgaaa gtgattgatt taaatgaccg ggttctcaat cttgacaacc cggatgataa
      961 aatgatctcc gcttttgcta actatgctgt acaaactgaa aattggcagc aaaatgcgtt
     1021 acaagcatta acgtctgata aaaaaggatt aactcctgaa aaattattag tcttgcagga
     1081 tcatgtgctt aattataatg tcgaggtttc attggtggga acattagcaa gaaaaattgt
     1141 cgctgcagtt gagacgttga cccgctcatg aaatatatat cattattatt gtttatcctg
     1201 ctgttgtgcg gctgcaagca gcaggaatta ctgaaccatc ttgatcaaca gcaagctaat
     1261 gatgtccttg ctgttttaca aagacataat attaatgctg aaaagaagga tcaaggcaaa
     1321 actggattct caatctatgt tgaaccaacg gattttgctt ctgcggtgga ttggctaaaa
     1381 atctacaatc tcccgggtaa gcctgatatt caaatatcgc agatgttccc tgcagatgca
     1441 ctcgtttcat ctccacgagc ggaaaaagca aggctatatt ctgctattga gcagcggtta
     1501 gagcaatctc tcaaaattat ggatggtatt gtttcttcga gagtacatgt tagttacgat
     1561 gttgatactg gcgatagtgg taaaactgct ttaccaattc atatttctgt acttgcagta
     1621 tatgaaaaag atatcaatcc agaaataaaa ataaatgaca taaagagatt tattgttaat
     1681 agttttgcaa gcgtacaata tgagaatatt tctgttgtat tgtcaaaacg aagagacatt
     1741 attgagcaag caccaacata tgaaattagc gaacctgttt ttgcttatga taaggctatg
     1801 ccggtttcta tcttacttgc attaatatcc gtagcgactt gctggctgtt atggaagtat
     1861 agggccattc taactaattt agtcaggttg aaaattaagt agacatatga gaacgttatg
     1921 aatcttgccc taagaaagat tatatatgct ccaattagtt atattcatcc tcaacgagta
     1981 agcttgaata atacacctat taacaatccg gtgctgagat ctattaccaa tgaaatgatt
     2041 ctcctgcagt ataatctttc agttgaacat tttaatctga atagttcact tatttactac
     2101 ataaacaatt ggaatttact tccattgatc tgtttactca gtggttgtca cttttatcga
     2161 gaaaggtttg cagaacgcgg gtttttttat aaggttcctg atgtactaag ggattattta
     2221 tcagctatac cgctggaaat aaatgaaaag gcacgataca aacccggcat cgctaactat
     2281 cacaatatca tcacatgtgg tttttcaacg ttgttaccct atataaggca acaaccattg
     2341 gcaatgcagc aacgatttaa tttattattt cctgattttg ttgaccacat actatcgccg
     2401 ttaccacttg catcaacact tttagagaga attacatttt atgcgaaaaa aaatagagat
     2461 gagcttgata aaatctcctg caaatggtgt tgtgattaaa agaaaaattt ctgatggatt
     2521 gaaagaaata gtttctctta aagaaaaaat ccttcttgaa accaccgcaa aaattcagag
     2581 tattgaggaa aagagagaag aaaaatttat tcaaggttat tatgatggct ata
//
LOCUS       DABSYT020000247         2627 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.249, whole genome shotgun sequence.
ACCESSION   DABSYT020000247 DABSYT020000000
VERSION     DABSYT020000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.249"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            229..645
                     /locus_tag="ID725_RS25440"
                     /old_locus_tag="pgaptmp_005088"
     CDS             229..645
                     /locus_tag="ID725_RS25440"
                     /old_locus_tag="pgaptmp_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGV7853477.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSIR
                     QNARLHAMLSEISKKATYHGKARNIEFWKGLFVSGWQIATNQHPEIISGLEGELINIR
                     ESTATLSVKKISEIMDYIEAYCAMNSIHLSEWRNYD"
     gene            638..1240
                     /locus_tag="ID725_RS25445"
                     /old_locus_tag="pgaptmp_005089"
     CDS             638..1240
                     /locus_tag="ID725_RS25445"
                     /old_locus_tag="pgaptmp_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HGV7853478.1"
                     /translation="MIEVWVDIEGIPFYQVSNKGNFRSITREVTVTSTRQRPYKKIIN
                     GTSVKPFKCKSTGYLQIKVYGKKYSAHRIVAKAFCTGFCDGLVVNHKNGRRDDNRADN
                     LEWVSHSENSKHGYKQNGRIPISLGKFSGDHPASKAVISTDMKTGEEVYYEAAMDAVR
                     EGFDSSSISRCCNGESSYHKGRFWRFANEKMKARWGDRAA"
     gene            1237..1764
                     /locus_tag="ID725_RS25450"
                     /old_locus_tag="pgaptmp_005090"
     CDS             1237..1764
                     /locus_tag="ID725_RS25450"
                     /old_locus_tag="pgaptmp_005090"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGV7853479.1"
                     /translation="MTIKSNTPAHNKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEVDDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1761..1955
                     /locus_tag="ID725_RS25455"
                     /old_locus_tag="pgaptmp_005091"
     CDS             1761..1955
                     /locus_tag="ID725_RS25455"
                     /old_locus_tag="pgaptmp_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGV7853480.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTRGLTR"
     gene            2212..>2627
                     /locus_tag="ID725_RS25460"
                     /old_locus_tag="pgaptmp_005092"
     CDS             2212..>2627
                     /locus_tag="ID725_RS25460"
                     /old_locus_tag="pgaptmp_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGV7853481.1"
                     /translation="MATKIAVETLSPITHNQIPVITTELLAHLYGTDVANIKMNHSRN
                     QTRFLEGKHYFKIVGDDLKNLRVTFSYLQISPKTRSLILWTERGAARHAKMLETDQAW
                     EVFEKLEDCYFSQCKKNTGKQEKKPNGLSAKETDSLV"
BASE COUNT          825 a          557 c          645 g          600 t
ORIGIN      
        1 actcaataat gagtttcagc gcaacagaat tatccggagt ctggatttaa agtgaaggag
       61 tgagcatgag cgagcaaata ttcagagaga ttaagccacg gttttatcgc aaggtaaggg
      121 tggtttatca ggacgaaaac aagacatgtg catacgccat tcataatggt cggtggtcag
      181 tgttcgacac caaaaacttc gagaagaact tcgagaggat taagggtgat gaggaaacta
      241 acatttgaac taagaagccc catccatcag cagaacgcca ttcaagctat ccagcaaatt
      301 cttccagacc caaccaaacc aatcgtagta accattcagg aacgcaaccg cagcattcgg
      361 caaaatgcac gccttcacgc gatgctatct gaaataagta agaaggctac atatcacgga
      421 aaagcaagaa atattgagtt ttggaagggg ttattcgttt ctggttggca gattgcaacc
      481 aaccagcacc ctgagattat atcagggtta gaaggtgagc taataaacat cagagagagt
      541 acggcgactc tatctgtaaa aaaaatatcc gaaataatgg actacataga agcatattgt
      601 gccatgaact caattcatct tagcgaatgg aggaattatg attgaggttt gggtagatat
      661 cgaagggatt ccattttatc aggttagcaa taaaggaaat ttcaggtcta ttacgaggga
      721 agttacagta acatcaacca gacagaggcc atataagaaa ataattaatg gcactagtgt
      781 aaaaccattc aagtgcaagt cgacaggata tcttcaaata aaggtatacg gtaagaaata
      841 cagcgcccac aggatagttg cgaaagcatt ctgtacaggg ttctgtgatg gcttggtagt
      901 taatcacaaa aatgggcgaa gagatgacaa tagggctgat aaccttgaat gggtatcaca
      961 ttctgaaaac tcaaaacacg gatataaaca aaatggaaga atacctatat cgctaggtaa
     1021 atttagtggt gaccatcctg ccagtaaagc tgttatttct actgacatga aaactgggga
     1081 ggaggtttat tatgaagcag ctatggatgc tgtcagagaa ggatttgata gttcgtcaat
     1141 tagtcgttgc tgtaatggcg aaagctcata tcacaaagga agattctggc gatttgcaaa
     1201 tgaaaaaatg aaagcgcgat ggggagatcg ggctgcatga ctatcaaatc aaatacgcca
     1261 gcacacaaca aggactgctg gcaaacgccg ctttggcttt ttgatgcact ggatattgag
     1321 tttggattct ggctggattc agctgcgagc gacaaaaatg ctctgtgcgc tcactggcta
     1381 actgaggtcg acgacgcgct caattctgag tgggtaagcc acggtgcaat ctggaataac
     1441 ccaccgtaca gcaatatcag gccgtgggtg gaaaaagccg ctgagcagtg catacaacag
     1501 cgacagacgg tagttatgct tgtgccagag gatatgtcag tcggatggtt cagcaaggct
     1561 ctggagagtg ttgacgaagt tcgcattatc actgatggac ggattaattt tatcgaacca
     1621 tcgacggggc tggagaagaa gggaaacagt aaaggctcca tgctgctgat ttggcgaccg
     1681 ttcatcagtc ctcgacggat gtttactacc gtatccaaag cggcattgat ggcgatcggg
     1741 cagggcgtca ggagggcggc atgaggcgac agcgacgaag tatcaccgac atcatctgcg
     1801 aaaactgcaa ataccttcca acgaaacgct ccagaaataa acgcaagcca atcccaaaag
     1861 aatctgacgt aaaaaccttc aattacacgg ctcacctgtg ggatatccgg tggctaagac
     1921 atcgtgcgag gaatacaagg ggattgacgc gatgatttat ccggggctat attcctcaca
     1981 cgccagcaaa atctggcgtc gggattggcg tcccggatga aaaaggcgac aacagacgcg
     2041 ccagcgtctt ttttattgtc gtttgcacag tcacatctca atggtgggct gtgtgggggc
     2101 ggagcaatcc gcgccggttc ctttttcccg gttacgccaa ccctgcacag ttcaccacca
     2161 agcgattggc gtcgcaggtg gtgatgattc acaaagaaaa aggatcatct tatggccacc
     2221 aaaatcgcag ttgaaactct ttccccgatt acccacaacc agatccctgt cataaccacc
     2281 gagctattgg cgcatcttta tggaacggat gttgccaaca taaaaatgaa tcattcacgt
     2341 aatcaaactc gttttctgga agggaagcat tatttcaaaa tcgttggcga tgatctgaaa
     2401 aatttgcgag taacttttag ttacctgcaa atttccccca aaacccgctc cctcatcctc
     2461 tggactgaac gcggcgctgc ccgtcacgcc aaaatgctcg aaaccgatca ggcgtgggaa
     2521 gtgttcgaaa aactggaaga ctgctatttc agccagtgca agaaaaatac tggcaaacaa
     2581 gagaagaagc ccaacgggct ttccgcaaaa gaaacagaca gccttgt
//
LOCUS       DABSYT020000248         2614 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.250, whole genome shotgun sequence.
ACCESSION   DABSYT020000248 DABSYT020000000
VERSION     DABSYT020000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.250"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            71..1186
                     /gene="adrA"
                     /locus_tag="ID725_RS25465"
                     /old_locus_tag="pgaptmp_005093"
                     /gene_synonym="dgcC"
     CDS             71..1186
                     /gene="adrA"
                     /locus_tag="ID725_RS25465"
                     /old_locus_tag="pgaptmp_005093"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGV7853482.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1203..2012)
                     /gene="proC"
                     /locus_tag="ID725_RS25470"
                     /old_locus_tag="pgaptmp_005094"
     CDS             complement(1203..2012)
                     /gene="proC"
                     /locus_tag="ID725_RS25470"
                     /old_locus_tag="pgaptmp_005094"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGV7853483.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2132..2590
                     /locus_tag="ID725_RS25475"
                     /old_locus_tag="pgaptmp_005095"
     CDS             2132..2590
                     /locus_tag="ID725_RS25475"
                     /old_locus_tag="pgaptmp_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGV7853484.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
BASE COUNT          583 a          665 c          732 g          634 t
ORIGIN      
        1 aatggctcac ccgaaatttc atacttctgc ctttagcccc gtctctataa tttgggaaaa
       61 ttgtttctga atgttcccaa aaataatgaa tgatgaaaac tttttcaaaa aagcggcggc
      121 gcacggggag gaacctcctt taactcctca aaacgaacat cagcggtccg ggctgcgctt
      181 cgcccgtcgc gtcagactac cccgtgcggt tggcctggct ggcatgttct taccgattgc
      241 ttcaacgctg gtttcacacc cgccgccggg ctggtggtgg ctggtgttgg tcggctgggc
      301 gttcgtctgg ccgcatttag cctggcagat agcgagcagg gccgtcgatc cgcttagccg
      361 ggaaatttac aacttaaaaa ccgatgcagt attagcggga atgtgggtag gcgtaatggg
      421 cgtaaacgtg ctgccttcca ccgcgatgtt gatgattatg tgtctgaatt tgatgggggc
      481 aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg gtggtttcct gccttgtcac
      541 cctcgagctg acgggcatta ccgtgtcgtt caatagtgcg ccgctggaat ggtggctctc
      601 ccttcccatt attgtcattt atcctctgct gtttggctgg gtcagctacc agacggcaac
      661 caaactggcg gaacataaac gcaggttgca ggtcatgagt acccgcgacg gcatgacggg
      721 cgtgtataac cgacgtcatt gggaaactat gttacgcaat gaatttgata actgtcggcg
      781 gcataatcgc gatgcaacgt tactgattat cgatatcgac catttcaaga gcatcaacga
      841 tacctggggc catgatgtgg gcgatgaagc gattgtggcg cttacccgac agttacaaat
      901 taccctgcgc ggtagcgatg tgattggtcg gtttggcggc gatgagtttg cggtaatcat
      961 gtccggtacg ccagctgaga gcgccattac cgccatgtta cgggtgcatg aagggctaaa
     1021 tacattacgt ttgccgaata cgccacaggt aaatttacgg attagtgtgg gggttgcgcc
     1081 gctgaaccca caaatgagtc actatcgcga gtggttgaaa tcggcagatt tggcgctcta
     1141 caaagcaaag aaagccggac gtaaccgcac cgaagtggcg gcctgacgtc cggcgaaagt
     1201 catcaggatt tgctgagttt ttctgatttt tccatacact tcgtcatcgc ttcgatcact
     1261 gcggcacgga agcctttctc ttccagtacg cgtaccgctt caatggtggt gcctcccggt
     1321 gagcagacca tatctttcag tgcccccgga tgttctcccg tttccagcac catttttgcg
     1381 gaacccatta ccgcctgagc ggcaaattta tacgcctggg cgcgtggcat cccgcccagc
     1441 acggcggcgt cggccatcgc ttcgataaac ataaagacgt aggctggcga agaaccgctc
     1501 acaccgacca ccgggtggat catcggctca gcaattactt ccgcttcgcc aaagcagcgg
     1561 aaaatattca gcacatcagc ggtatcttct ggggttacca gcgcgtttgg cgttacggag
     1621 gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg cgcggataat tttccggtca
     1681 tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg cagcaataga aacgaccaga
     1741 gagtctttat tgaggctgga ggtgatttcg ctaagcactt taatcatgat gccaggttta
     1801 acggcggcaa aaatgatgtc ggcgatttgc gccacttctt gcgccgattc tgcggcgttg
     1861 atgccgaact tgtcatgcag ggcggcgact ttatccgggg agggggtata tacccagatt
     1921 tgccctggaa gcacctgacc gctggcaatc agaccgccga gaatggcttt tcccatattg
     1981 ccgcagccaa taaaaccgat tttcttttcc attgcctcac tcctgccgtg aaattcattg
     2041 ttttgataat cgctggcaga agcataaaca gaactatgcc ggaaggcaaa agcgcgacac
     2101 aatagaggat tacccaacaa aggatgactt tatgacaatt tgggtggatg ccgacgcgtg
     2161 tcccaatgta attaaagaga ttttgtatcg cgcggcggaa cgtatgcaga tgccgctggt
     2221 actggtagca aaccagagtt tacgcgtgcc gccatcgcga tttattcgta cgctgcgcgt
     2281 cgcggcaggt ttcgacgttg ccgataacga aattgtccgg cagtgtgaag cgggagattt
     2341 ggtgatcacc gcagatatac ctttggctgc tgaagccatc gagaaaggcg ctgcggcgct
     2401 taatccgcgc ggtgaacgtt acacgccagc gaccattcgt gagcgcctga cgatgcgcga
     2461 ttttatggat accttacgtg ccagtgggat ccagaccggc ggaccagata gcctttcaca
     2521 acgtgaccgc caggcctttg ccgcggagct ggagaagtgg tggctggaag tgcaacgtag
     2581 tcgtggctaa atgtaattta ttatttacac ttca
//
LOCUS       DABSYT020000249         2610 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.251, whole genome shotgun sequence.
ACCESSION   DABSYT020000249 DABSYT020000000
VERSION     DABSYT020000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.251"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(106..540)
                     /locus_tag="ID725_RS25480"
                     /old_locus_tag="pgaptmp_005096"
     CDS             complement(106..540)
                     /locus_tag="ID725_RS25480"
                     /old_locus_tag="pgaptmp_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGV7853485.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(533..727)
                     /locus_tag="ID725_RS25485"
                     /old_locus_tag="pgaptmp_005097"
     CDS             complement(533..727)
                     /locus_tag="ID725_RS25485"
                     /old_locus_tag="pgaptmp_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGV7853486.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            complement(724..1329)
                     /locus_tag="ID725_RS25490"
                     /old_locus_tag="pgaptmp_005098"
     CDS             complement(724..1329)
                     /locus_tag="ID725_RS25490"
                     /old_locus_tag="pgaptmp_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGV7853487.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAATPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            complement(1329..2051)
                     /locus_tag="ID725_RS25495"
                     /old_locus_tag="pgaptmp_005099"
     CDS             complement(1329..2051)
                     /locus_tag="ID725_RS25495"
                     /old_locus_tag="pgaptmp_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGV7853488.1"
                     /translation="MSELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(2044..2253)
                     /locus_tag="ID725_RS25500"
                     /old_locus_tag="pgaptmp_005100"
     CDS             complement(2044..2253)
                     /locus_tag="ID725_RS25500"
                     /old_locus_tag="pgaptmp_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGV7853489.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCSDCCAELMSDPNSSMYEEEDDE"
     gene            complement(2213..>2610)
                     /locus_tag="ID725_RS25505"
                     /old_locus_tag="pgaptmp_005101"
     CDS             complement(2213..>2610)
                     /locus_tag="ID725_RS25505"
                     /old_locus_tag="pgaptmp_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853490.1"
                     /translation="LIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREATN
                     YWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYKKAWKEHRDRYQQDMEKLES
                     ENMELRRKLGEAKRDIDAYKRLFNGESHA"
BASE COUNT          560 a          686 c          562 g          802 t
ORIGIN      
        1 tgtactttcc gtatagctca caggattcgg ggtacaaaaa aacccgcgca tcggcgggtt
       61 cgactgcgtg gcaatgtaac cactcttatc atgatatgca gatttttacg atcgtaaact
      121 attttttcgc tgataaaata cagaggttct ccctcccggc aattcacgct caacataccg
      181 atccatctca agcctcactc ccagcatcat cagcatgcct tcaacaatcc cctccgcttt
      241 gtgaaggcgt ttacctatac aggtgtcaga gcacccatgt ttccgtgcca acgccatgaa
      301 cgtctccccc aacacgtaat aatcaaccag caagtcatgc agatcgcgat tgttccggta
      361 aaggcgggct atgcacccgc atatcaccat cgcatcatcg tcacagcact gtggacgtga
      421 ttttactttt tcggggatca gtcccttaaa tccggcagca atgggcgacc atgtaacatc
      481 ctcatagtta tttgccgccc atgcccccca gcgctcaaga acctgccgga tatcacgcat
      541 cagtatcttt accccatccg cggtgaacca taagaacacc gttgacgata gcgtgtcttt
      601 tcccttcttt atcgccagtg tattttctga ccgtgttgcg actacagttc agtattctgg
      661 ctacctcggt ctgattttca tatgcctcaa cgagcatgtc aggaatggtt tttactgtga
      721 acgtcatgcg gcctcacttc tgctgtttcg caggtcttta agtttctgct gatacttcgc
      781 cttgatcgcc ctgcattctt cgacagtcca gcgatggcgg ttatggttcg attcgatttc
      841 gtctactgct tcctgcccga tgcggttaat cagttcgacg cgatacggaa cgagatttcc
      901 gcttttgtgc tggttgcaca ccacgcattg cttgtgaata ttgcgttcat caaatcggag
      961 ttgaggtgtc gcagcagttg tccggtaatg tccggcatcc cactgagcag acgtgagcgt
     1021 tccgcacgag atacatggta agtcgcggtc tctttctctg atgaaggcgt ttacggcttg
     1081 ttgggcttgt ttaatccagt aactgcgggg ctttaaggcg agttttcgaa tcttcagttt
     1141 atctttctgt ttctgctcct ctcgtcgtcg tttcttctct gctgcttttt ccgctttttc
     1201 gcgttcttta cttcgtcgtt cgagtgctaa ttgagttccg tgttccgggc agcaccacca
     1261 ctgatttgag aatgccgggt gaaaccattc cttacagatt ttgcatttcc ttcgcgctgg
     1321 tttagccatt aagcagcctc ccctgttact ttaagcattc cgttatctag cagctttctt
     1381 gtcagccact gttgaccacg cccggtgatt tttgtggtga acgatatctg tattccgtga
     1441 tttgtattga ccgctgtttc tttcactgtg aaatagccgc gatccatata ttcctgcatt
     1501 ggcacattgc gccgggcacc tgaagcaata aggattttgt gatcgcgcat ccacgcaaac
     1561 agtttgtttg gaccaatacc aacaaccttt gcaaagtttc caatcaaaat tccgctggcc
     1621 tcgccaacgc gatcggcaaa ctcaacttta ggtgcggcaa ttgcgagctg gttttccagt
     1681 tgcattttct gctcagcaag atcagcagca aggcgcaacg cttctggtag cgttttgggg
     1741 atattaaccg cagcttcttc aagctctcgc cagcggtcaa caaggcgagc ggtgaattcc
     1801 ggcgacagct gggctacaac gacaatactg tctcgctttc cttgttcgcc ttcgaagacg
     1861 taatgctcgt actgaacatt gaaccctaag ttattgattc tttcggaaac ctcaatttga
     1921 ggaagccgga taacaccatt tttagccagc gtttcgatgg tacgtttcac attgtcatga
     1981 cgcttaccca ccaactcagc gatttcaatg cttgtcattt tgatggcatt gccatttatt
     2041 aactcactca tcgtcttctt cctcgtacat tgagctattc ggatcgctca tcagttctgc
     2101 gcagcaatcg gagcacacgt gaacttccag cacatgcagc ttctgaccgc agttagcgca
     2161 cgttaaagcc cgctcgacgc tttctttctg gtattgaagg gattgggatg gactaagcat
     2221 ggctttcacc attaaaaagt cgcttgtaag catcaatgtc tcgttttgct tcaccgagct
     2281 ttcgtcttaa ttccatgttt tctgattcaa gcttttccat gtcttgctgg tatcggtcgc
     2341 ggtgttcttt ccatgctttt ttatacgcct tcatgtatgt catgttggcc tttctctttg
     2401 cctgacgaac tgcgtggtgg tttttcacaa accagtcagg gtcgttaaat gctgctctgg
     2461 cgcaggtata ccaataattt gttgcctccc tgtttagcca ataaatactg ataaatggca
     2521 accggataga caccattttt cgttgagact ctttctcgcc aaacatgtgc ccttttttga
     2581 tgctaaggcc aaatccaggt tgaattaaaa
//
LOCUS       DABSYT020000250         2584 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.252, whole genome shotgun sequence.
ACCESSION   DABSYT020000250 DABSYT020000000
VERSION     DABSYT020000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.252"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..353)
                     /locus_tag="ID725_RS25510"
                     /old_locus_tag="pgaptmp_005102"
     CDS             complement(<1..353)
                     /locus_tag="ID725_RS25510"
                     /old_locus_tag="pgaptmp_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853491.1"
                     /translation="MKKAAILIDAGFFMQRVHATHRKHFAEHELTAQCIMKVIWSMVL
                     SHLNGKRQSQERREPLELYRIYFYDCPPLDIQTRLPLPEPGNKTPGRKNFKLEKSYIL
                     RTELHEELRKTRKTA"
     gene            complement(481..1194)
                     /locus_tag="ID725_RS25515"
                     /old_locus_tag="pgaptmp_005103"
     CDS             complement(481..1194)
                     /locus_tag="ID725_RS25515"
                     /old_locus_tag="pgaptmp_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGV7853492.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            1295..1495
                     /locus_tag="ID725_RS25520"
                     /old_locus_tag="pgaptmp_005104"
     CDS             1295..1495
                     /locus_tag="ID725_RS25520"
                     /old_locus_tag="pgaptmp_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGV7853493.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            1614..1907
                     /locus_tag="ID725_RS25525"
                     /old_locus_tag="pgaptmp_005105"
     CDS             1614..1907
                     /locus_tag="ID725_RS25525"
                     /old_locus_tag="pgaptmp_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HGV7853494.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            1940..2584
                     /locus_tag="ID725_RS25530"
                     /old_locus_tag="pgaptmp_005106"
                     /pseudo
     CDS             1940..2584
                     /locus_tag="ID725_RS25530"
                     /old_locus_tag="pgaptmp_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT          761 a          575 c          580 g          668 t
ORIGIN      
        1 atggctgttt ttcgagtttt tcttaactct tcatgcagct ccgttctcag aatatatgat
       61 ttttcgagtt tgaaattctt gcgaccaggc gtcttattgc caggctcagg aagtggaagg
      121 cgtgtttgaa tgtcgagtgg tggacagtca tagaagtaaa ttctataaag ctcaagcggt
      181 tccctacgtt cttgtgattg acgttttcca ttaagatggg aaagaaccat tgaccatatt
      241 actttcatta tgcattgcgc agtcagttca tgctcggcga agtgtttacg atgcgtagca
      301 tgaacacgct gcatgaaaaa acccgcatca attaaaattg ctgctttctt cataagagtc
      361 ccaaaaaaat agcccagagc cgttatgcag atattaacaa ttgtctgcga acggggctgg
      421 gcttggttta attaatctat gccaagttga ctgtgccgtc aacataaaac ttacttccta
      481 tcacccaaac gtctcttcag gccactgact agcgataact ttccccacaa cggaacaact
      541 ctcattgcat gggatcattg ggtattgtgg gtttagtggc tgtagaaaca cctgaccgct
      601 atccctgatc agtttcttga aggtaaactc atcaccacca agtctggcta tacagaaatc
      661 gcctggctca acagcctgct cagggtcaac cagaattaac atcccgtcag gaaagctggg
      721 cttggatcct gttggtgcgg tcatggaatt accttcaacc tcaagccaga atgcagagcc
      781 actggctttt ttggttgtgc ttacccattt ctccgcatcg cctttggtaa aggttctaag
      841 ctcaggcgag aacatcccgg cctgaacatg agaaaaaaca gggtactcat actcacttct
      901 aagtgacggc tgcatactaa ccgcttcata catctcgtag atttctctgg cgattgaagg
      961 gctaaattct tcaacgctaa cgttgagaat ttttgcaagc aatgcggcgt tataagcatt
     1021 taatgcattg atgccattaa ataaagcacc aacgcctgac tgccccatcc ccatcttgtc
     1081 tgcgacagat tcctgggata agccaagttc atttttcttt ttttcataaa tagctttaag
     1141 gcgacgtgcg tcctcaagct gctcttgtgt taatggtttc ttttttgcgc tcatacgtta
     1201 aatctatcac cgcaagggat aaatatctaa caccgcgcgt gttgactgct ttacctctgg
     1261 cggtgataat ggttgcatgt actaaggagg ttgtatggaa caacgcataa ccctgaaaga
     1321 ttatgcaatg cgctttgggc aaaccaagac agctaaagat ctcggcgtat atcaaagcgc
     1381 gatcaacaag gccattcatg caggccgaaa gattttttta actataaacg ctgatggaag
     1441 cgtttatgcg gaagaggtaa agcccttccc gagtaacaaa aaaacaacag cataaataac
     1501 cccgctctta cacattccag ccctgaaaaa gggcatccaa ttaaaccaca cctatggtgt
     1561 atgcatttat ttgcatacat tcaatcaatt gttatctaag gaaatactta catatggttc
     1621 gtgcaaacaa acgcaacgag gctctacgaa tcgagagtgc gttgcttaac aaaatcgcaa
     1681 tgcttggaac tgagaagaca gcggaagctg tgggagttga taagtcgcag atcagcaggt
     1741 ggaagagaga ctggattcca aagttctcaa tgctgcttgc tgttcttgaa tggggggtcg
     1801 ttgacgacga catggctcga ttggcgcgac aagttgctgc gattctcacc aataaaaaac
     1861 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1921 tctatcaaca ggagtcatta tgacaaatac agcaaaaata ctcaacttcg gcagaggtaa
     1981 ctttgccgga caggagcgta atgtggcaga tctcgatgat ggttacgcca gactatcaaa
     2041 tatgctgctt gaggcttatt cgggcgcaga tctgaccaag cgacagttta aagtgctgct
     2101 tgccattctg cgtaaaacct atgggtggaa taaaccaatg gacagaatca ccgattctca
     2161 acttagcgag attacaaagt tacctgtcaa acggtgcaat gaagccaagt tagaactcgt
     2221 cagaatgaat attatcaagc agcaaggcgg catgtttgga ccaaataaaa acatctcaga
     2281 atggtgtatc cctcaaaacg agggaaaatc ccctaaaacg agggataaaa catccctcaa
     2341 attgggggat tgctatccct caaattgggg gattgctatc cctcaaaaca gggggacaca
     2401 aaagacacta ttacaaaaga aaaaagaaaa gattattcgt ccgagaattc tggcgaatcc
     2461 tctgaccagc cagaaaacga tctttctgtg gttaaaccgg atgctgcaat tcagagcggc
     2521 agcaagtggg gaacagcaga agacctgacc gccgcagagt ggatgtttga catggtgaag
     2581 acca
//
LOCUS       DABSYT020000251         2547 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.253, whole genome shotgun sequence.
ACCESSION   DABSYT020000251 DABSYT020000000
VERSION     DABSYT020000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.253"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..480
                     /locus_tag="ID725_RS25535"
                     /old_locus_tag="pgaptmp_005107"
     CDS             <1..480
                     /locus_tag="ID725_RS25535"
                     /old_locus_tag="pgaptmp_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGV7853495.1"
                     /translation="SARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVN
                     FVAGNPEADPEQELRDRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMW
                     TYRSDECGYTGGAVADEFDNPTTDIRKDRCSKCMRGCEMRSMVANFGGFLSINKLSQ"
     gene            491..1234
                     /locus_tag="ID725_RS25540"
                     /old_locus_tag="pgaptmp_005108"
     CDS             491..1234
                     /locus_tag="ID725_RS25540"
                     /old_locus_tag="pgaptmp_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGV7853496.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTDMPDFEREDDWWRNGQNLYLDNMEAT
                     GFYRISLPSAQPGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            1231..1812
                     /locus_tag="ID725_RS25545"
                     /old_locus_tag="pgaptmp_005109"
     CDS             1231..1812
                     /locus_tag="ID725_RS25545"
                     /old_locus_tag="pgaptmp_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGV7853497.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            2154..>2547
                     /locus_tag="ID725_RS25550"
                     /old_locus_tag="pgaptmp_005110"
     CDS             2154..>2547
                     /locus_tag="ID725_RS25550"
                     /old_locus_tag="pgaptmp_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853498.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRN"
BASE COUNT          554 a          623 c          823 g          547 t
ORIGIN      
        1 agtgcccgcc cgtcgctgac ggtgtccaat ctgttcggtc ttgtcaccgg gatggcggag
       61 gatttgcaga gcctggtggg tgccacggtg gtccgccgcc gggtgtatgc ccgttttctg
      121 gatgcggtga attttgtggc gggcaatccg gaagcggacc cggagcagga gctgagagac
      181 cgctgggtgg tggagcagat gtcagagctg acggccatga cagcctcgtt tgtgctggca
      241 acaccgacgg agacggacgg agcgctgttt cccggtcgca ttatgctggc gaatacctgt
      301 atgtggacct accgctctga tgagtgtggt tacacgggcg gggcagtggc ggatgagttc
      361 gacaacccca ccacggatat ccgtaaggac agatgcagca agtgcatgcg cgggtgtgag
      421 atgcgcagca tggtggctaa ttttggcggt ttcctttcca ttaataaact ttcgcagtaa
      481 atcccggttt atgacacaga ctgaatcagc gattctggcg catgcccggc ggtgtgcgcc
      541 tgcggagtcg tgcggcttcg tgataagcac gccggagggg gagtggtata tcccttgtgt
      601 gaatatttct gcagagccgg aggcgtattt tcgtatcgca ccggaagact ggctgcgggc
      661 agagatgcag ggggagattg tggcactggt ccacagtcat cccggtgggc tgccctggct
      721 gagcgaggct gaccggcggc tgcagataaa aagcgcactg ccctggtggc tggtctgccg
      781 gggtgacatt cacaaattcc gctgtgtgcc acatctgaca ggacggcgct ttgagcacgg
      841 ggtgacggac tgttacacgc tgttccggga tgcttatcat ctggcgggga ctgatatgcc
      901 ggattttgag cgtgaggatg actggtggcg caatggtcag aacctgtacc tggacaatat
      961 ggaggcgact ggtttttaca ggatttccct gccttccgca cagcctggcg atatcctgct
     1021 gtgctgcttt ggcgcatcgg tggccaatca tgccgccata tactgcggca acggtgaact
     1081 gcttcaccat ctgcctgaac aactgagtaa acgggagagg tattccgaaa aatggcaacg
     1141 acgaacgcat tcagcctggc gtcaccgcca ctggcacgta tctgccttca cggggattta
     1201 caacgatttg gccgccgcct cagcctgtat gtgaacacgg cagcggaagc catccgggcg
     1261 ctgtcgttac aggtgccggg ctttcgccgt cagatgaacg aaggctggta ccagatacgt
     1321 attgccggtt atgacacggc accggaggcg gtgtacgccc gtcttcacga acagctgggt
     1381 gagggaacgg tcatccatat tgtgccgcga ctggccgggg ccggaaaggg tggactgcag
     1441 attgtgctgg gggcggcagc catcgtgggc tctttcttca ctgccggggc atcaatggcg
     1501 ttatggggtt cagccctggc agccggtggt ttttctgcca ccacgatgct gttttcactt
     1561 ggagccagca tgattctggg cggtgtggcc cagatgctgg ccccgaaggc aaaaacaccg
     1621 gattaccgcg caacggataa cggcagacag aacacgtact tttcctcgct ggataacatg
     1681 attgcccagg ggaacccgat gccggtgcct tacggggaaa tgctggttgg ctcccgccgt
     1741 atatcccagg acatcagcac ccgtgatgaa ggtgggggcg gaacggtcgt ggttgtcggg
     1801 cgacagggat aaaacataaa aaaatcccgc agtgatcgcg gagctgcggg gacagacaaa
     1861 tgaagatcaa tgttaaggag ttgtttttgt tactcgggca aaaaaacact aacgcagcga
     1921 aattataagc gccacagtca gtgtgtgaaa atgtgaagat attcagaatt tttatgccat
     1981 taccggtttt aaccaacagg attatcggtg ggcatgaaag aaaaccccgg tatctgctga
     2041 taccggggtt ttctttagca tggcagaaat gtgtttcatg cttttcgggc gaaggatatc
     2101 cgacttctgt acggaatggc aagtggcggt taatttattc aggggaaggc tgtatgggaa
     2161 aaggtggcgg taaggcacac acgcctcgtg aggcgaagga taatctcaaa tccacgcaga
     2221 tgatgagtgt gattgatgcg attggtgagg gaccgataga aggtccggtg aagggactgc
     2281 agagtattct ggtgaacaaa accccactga cggacacgga cggcaatccc gtgatacacg
     2341 gtgtgaccgc ggtctggcgt gccggggagc aggagcagac accaccggaa ggctttgagt
     2401 cctccggagc tgaaaccgga ctgggcgtgg aagtgacgaa ggcaaaaccg gtgacgcgca
     2461 ccattacgtc cgcgaacatt gaccgcctgc gggtcacctt cggggtgcag tcactgttgg
     2521 agaccacctc aaagggcgac cgtaatc
//
LOCUS       DABSYT020000252         2520 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.254, whole genome shotgun sequence.
ACCESSION   DABSYT020000252 DABSYT020000000
VERSION     DABSYT020000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.254"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(1..1712)
                     /locus_tag="ID725_RS25555"
                     /old_locus_tag="pgaptmp_005111"
                     /pseudo
     CDS             complement(1..1712)
                     /locus_tag="ID725_RS25555"
                     /old_locus_tag="pgaptmp_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1658..1879)
                     /locus_tag="ID725_RS25560"
                     /old_locus_tag="pgaptmp_005112"
     CDS             complement(1658..1879)
                     /locus_tag="ID725_RS25560"
                     /old_locus_tag="pgaptmp_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853499.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGDPEKTRNQDVM"
     gene            complement(1924..>2520)
                     /locus_tag="ID725_RS25565"
                     /old_locus_tag="pgaptmp_005113"
     CDS             complement(1924..>2520)
                     /locus_tag="ID725_RS25565"
                     /old_locus_tag="pgaptmp_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGV7853500.1"
                     /translation="ALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPD
                     ADAEAAFGQFVTANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLP
                     VIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSM
                     FQTGSVAIRAERWINWRRRRTAAVAVITGVNYGTASGG"
BASE COUNT          508 a          829 c          622 g          561 t
ORIGIN      
        1 gtcaatggcg gggagtattt ccagtgccgt ggcatcacgc ccgtcctgcg gagccggcag
       61 ggcactcacc gcatcattca ccgccttctg cagaacatcc ggatcgtagt cacgaccgtc
      121 gcgcggaaca ggaatatggc tcactgcctc tttcaccatc tgctcaagca tcggacgcac
      181 atcatcaccc gtcacgcact tctgtaatac cacagactga gaagccagtt tttcttcaaa
      241 cgtttgtgcc tgcgcggcta ttttctcctc aaatgtacgc tgtaaatccg ccagcaccat
      301 ggcgaattct tcgcccagtg cacgaataat ggacagttcc cgttccgtca ttttcgcagt
      361 atccccctga acatcgcttt caccgcatca tgctctgttt cactgattgc cttattaccg
      421 tcagatgcgc catcaggcag ttgtgctgag actgttttcc cggacgacgc gaacggatcc
      481 tcacgggcat cacgacggga cagcgcctcc agactgtagt tctgctgctg aagatacagt
      541 gcatcaccgc cggccagggg cggcaggttc tcacgtttac gggcctcatt gggcgtcagc
      601 agcgtatttt tcaccgactc acccagcgtt ttcatgcgcc gttcgctgtc cattctcagc
      661 agcgtggtga cgtcaaactc cgtgctctcg ttttcccccg tttccagcgc ctcatccagt
      721 aacagctcaa tggactcaat cagcgtctgc agacactggg aataatactg ctgctccagc
      781 gcctccacgt tatcactgga gggaggttgt cccacaccaa tcttgtaggc cgggacacgg
      841 aacaccgaac agacaatttc agcggtcatt ttcagttgtt ccaccgtctg cgcatccacc
      901 ggtgaaaacg tcgtggggtt atatttcgcc ccgttgctca gaatcgccgt tttccccgca
      961 ttttcgccgg tatacccgct gtcccagttg ctcttcagtt ttttcgcatt ttcttccgtt
     1021 atactgccgg ggatctcaat caccccggac ggcctgccgc catttctgaa aaaagacgtt
     1081 gaatttgcct gaatatgata cccctgcgtg gcagccagcc cggcagcata caccggcggc
     1141 agccccacaa gcggatgaaa aaaacagtta aaccggtcgt ggatcacttc ccgggcaggc
     1201 accgtcaccg cctccgtgat cccgcagttc cggtccggcg tgatgcgata gaacacctcg
     1261 ccgtcatccg ccaccagagg ttcaacccgg ctccagtcca gaatacgcag ttctttgatc
     1321 tgcccacggg agttacggat tttcagcacc accgtattgc cgtgacgcag tttggcgttc
     1381 agccacagtt caaaaaactg gatacgattc tgctgtgcat tgggacgacg acagagacgg
     1441 gcaatatccc cctgccgttt ttcacggcgg atcccctgtg tatcggtctg catcaggcgc
     1501 agccgcattt tggcgatatc ctgggatatc agcgaaatgc aagaaaacac cgcatgaaag
     1561 gagaggacac tttccggatc ggctttcact ccctgctgcc aggcaccggc aaaaggctca
     1621 gccaccgcct gaaacaggct ggtccagccc acctctttta catcacgtcc tgatttctgg
     1681 ttttttcggg gtcgccgcaa aaggttccac attcgccatg ctccgcatca cgtttctttt
     1741 tctgacctgc cggacgtcgc gctgtgatgt actccgcctt ccccaggcga accagcacct
     1801 ccgcacacgg ctgtgccaca tcacggatat ccccggcccg ggcatcatgc gtgccctgca
     1861 gatactggat ttttgccatc agttactgcg ggaagctagc gcctcccgcc ctcctcatca
     1921 gactcagccg ccggacgcag ttccgtagtt cacaccggtg atcacagcca ccgccgcggt
     1981 acggcgacga cgccagttga tccagcgctc cgcacggatg gccacgctgc cggtctggaa
     2041 catggagacc agctccaccg gtgacggtgt gctgctgtcg ctggtcggtt cagactgcat
     2101 ctccagtgac gcttcacggg acatatccac cgccacacca ccgtcatccg ccagataaat
     2161 atccggcgca ttcaccagta ccagctggtc acccacatac tgggagacaa tcaccggcag
     2221 cccctggaag gtcccgccca gcagggtcat gtccggatat tccttctgcc ccagcgcatt
     2281 tttacgcatg gacagcgcca gggcattcgt gctggacatc agccagaccg caccggtggg
     2341 ctgcagattt gccgtcacaa actggccaaa cgcggcctcg gcatccgcat ccggattacc
     2401 gcttgacgcc gtgcccttca catcatgggt gatggacgca ggggagacat ctgccaccgc
     2461 cgcttttttc gggtccacaa agtctgtatc cagacgcgcc accaccgctt ccgccagcgc
//
LOCUS       DABSYT020000253         2517 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.255, whole genome shotgun sequence.
ACCESSION   DABSYT020000253 DABSYT020000000
VERSION     DABSYT020000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.255"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            90..1318
                     /locus_tag="ID725_RS25570"
                     /old_locus_tag="pgaptmp_005114"
                     /pseudo
     CDS             90..1318
                     /locus_tag="ID725_RS25570"
                     /old_locus_tag="pgaptmp_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            1329..1484
                     /locus_tag="ID725_RS25575"
                     /old_locus_tag="pgaptmp_005115"
                     /pseudo
     CDS             1329..1484
                     /locus_tag="ID725_RS25575"
                     /old_locus_tag="pgaptmp_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein GogB"
     gene            complement(1861..>2517)
                     /locus_tag="ID725_RS25580"
                     /old_locus_tag="pgaptmp_005116"
     CDS             complement(1861..>2517)
                     /locus_tag="ID725_RS25580"
                     /old_locus_tag="pgaptmp_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HGV7853501.1"
                     /translation="SINNKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQLREG
                     DIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDSDIIL
                     CRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLSVADR
                     FSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMVNASK
                     "
BASE COUNT          717 a          563 c          596 g          641 t
ORIGIN      
        1 gactggcccc ctgaatctcc agacaaccag tatcacttaa ataagtgata gtcttaatac
       61 tagtttttag actagtcatt ggagaacaga tgattgatgt cttagggccg gagaaacgca
      121 gacggcgtac cacacaggaa aagatcgcaa ttgttcagca gagctttgaa ccggggatga
      181 cggtctccct cgttgcccgg caacatggtg tagcagccag ccagttattt ctctggcgta
      241 agcaatacta ggaaggaagt cttactgctg tcgccgccgg agaacaggtt gttcctgcct
      301 ctgaacttgc tgccgccatg aagcagatta aagaactcca gcgcctgctc ggcaagaaaa
      361 cgatggaaaa tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa aagtggatag
      421 cgcacgcgcc cttattgccc ggggatgggg agtaagctta gtcagccgtt gtctccgggt
      481 gtcgcgtgcg cagttgtacg tcattctcag acgaaccgat gactggatgg atggccgccg
      541 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
      601 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      661 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      721 tgagcgaaaa cctactgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      781 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      841 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      901 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      961 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
     1021 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
     1081 ggtgcggagt ccggagagta acggaatagc agagaacttc gtgaaaacga taaagcgtga
     1141 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
     1201 gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct cgccacggga
     1261 atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg aaatataggg
     1321 gcaaatccag atggatttat cgtaataaag acaacagcca aataatgaaa aacataaaat
     1381 tttatctgca tggcaaagag ataccagcag aaagaatatt agatacacca gagtggaaag
     1441 actaccgtcc aaaatactcc ggttccacat ataaatattc ttaatgatag caaaaaatat
     1501 attttcgata taatcaatgt tatgattaaa gagtatttca tcagggcagg taaaaacaga
     1561 gtaaatcagc aaaagaagct gatcttcagc gatactgaca ctaactgacg gtttaagcgg
     1621 tcgtatgaag cagcagcttt ccgacggact gccatgcgga tcgtttacct tttgggctat
     1681 tccgcccgtc atcaagcggc tcacgagtac tgagtttatc aggatgatat tgctgacaat
     1741 ggtaattcgt tgaccgatgt gtacttcact acatatcggt caacaccgta ctgccgtgca
     1801 acatctccaa gatgtagcat cgtttttacc agaggaggca ggacatagat tgtaaaaaaa
     1861 ctatttcgat gcatttacca ttgagatatt ttccttcata ccaaaagttc tatcaattat
     1921 cgcaagtggt tcaacaggtg cggtcttatc tataacccaa ccactctctg taacacttcc
     1981 tcgtgtgata tattttgcct ttactttaac aactattaga taatgctcac gactaaaccg
     2041 atcagcaacg ctaagatccg atgtgtattc aggcaatatt cttccaatac gtacctgctg
     2101 ttgtgccata tattcatctg gtgcttttac atttggattt gaatcaggaa gccctccagc
     2161 agttttatat tcaacgatat tttcagcttg aataccggtt gtgccgcgac acaatatgat
     2221 gtctgaatcg gacagttcta tgtgcccctc cttaattttc gaataaattt gtttaatact
     2281 atgttctgga gttggtacta tttcagaaga taaagcaggc aacgtgtctt caagcaccgg
     2341 agaatcatcg tatatatcca acaaatcctc atcataccca atatctcctt ctcttaactg
     2401 agaatacggt ttatctatgt atctaataac tcccccacca tgaagacctt ctgatttata
     2461 aaatgtacca tcagcgtcac gaaagaaata aacattaacc tgtttgttat ttataga
//
LOCUS       DABSYT020000254         2505 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.256, whole genome shotgun sequence.
ACCESSION   DABSYT020000254 DABSYT020000000
VERSION     DABSYT020000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.256"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..346)
                     /locus_tag="ID725_RS25585"
                     /old_locus_tag="pgaptmp_005117"
     CDS             complement(<1..346)
                     /locus_tag="ID725_RS25585"
                     /old_locus_tag="pgaptmp_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HGV7853502.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKLKFDS"
     gene            complement(355..1122)
                     /gene="sctT"
                     /locus_tag="ID725_RS25590"
                     /old_locus_tag="pgaptmp_005118"
                     /pseudo
     CDS             complement(355..1122)
                     /gene="sctT"
                     /locus_tag="ID725_RS25590"
                     /old_locus_tag="pgaptmp_005118"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(1124..1384)
                     /locus_tag="ID725_RS25595"
                     /old_locus_tag="pgaptmp_005119"
     CDS             complement(1124..1384)
                     /locus_tag="ID725_RS25595"
                     /old_locus_tag="pgaptmp_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGV7853503.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1395..2060)
                     /locus_tag="ID725_RS25600"
                     /old_locus_tag="pgaptmp_005120"
     CDS             complement(1395..2060)
                     /locus_tag="ID725_RS25600"
                     /old_locus_tag="pgaptmp_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGV7853504.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2050..>2505)
                     /locus_tag="ID725_RS25605"
                     /old_locus_tag="pgaptmp_005121"
     CDS             complement(2050..>2505)
                     /locus_tag="ID725_RS25605"
                     /old_locus_tag="pgaptmp_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HGV7853505.1"
                     /translation="RTIVDIEVGDVLLISNNMAYAVIYNTKICDLIYPEELKMADHFQ
                     YEEDFETDDFDIKKSESEIYDENDEQMINSFEELPVKIEFVLGKKIMNLYEIDELCAK
                     RIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGHNNVK"
BASE COUNT          865 a          522 c          377 g          741 t
ORIGIN      
        1 atgaatcaaa ctttaatttt acagcttcag ttgctaattg cattttactt tgaaaccagg
       61 agacaagtat tgttgtgaac atgcaaacca gcaaaaaagg aatgatcgtt ttcaaccaac
      121 caatccccat agctttaaaa tagtcccaaa tatccggttt agcgttatga tcaaggatat
      181 attcaagaat cgacataatg tgatgcacat caaaaacata tccgagatat aaagtaccca
      241 caagcattat tacagaaacg gttaagtctc tactttttag aatttgccct tttttagaag
      301 catcctgcag tttcttttgt gttggctttt cagttttatt tgccatgaaa cactctattt
      361 ctcaatgtat agttgcaatt gttctgggaa aaaggataac ggcatcactc gttcagccag
      421 tattgggcca aaataaataa ttaatattaa aaatgccagc gcacttttga cagtcagtga
      481 aatggcaaaa gcatttagtt gtgaagcata gcgtgccagc aatcccagga cagcctcacc
      541 acccagcata acggcaatta cggggcttgc gtaaacgata gtatgagtca ttatattatt
      601 aatatatgaa aataaaggct ctaatttagg aaaattaaaa cttcctgaag gccataaatt
      661 ataactttgc taaagagttt ccagaataaa gttcaatcca ccgttagtaa gataaaccgc
      721 tgcagagaaa aggttaaata atcttgctaa ttccgaggta tcgacacctg tagccggatc
      781 caaagtacta cttaacgttg caccgcgttg gttatcgata aagctaccta tagcaaggaa
      841 aatccaaaat ggagatgcta ataagcaagc aatgaacagc cctatgataa tttctcttgc
      901 agctaacaaa gcgatatcga tttcatttaa ggtcgataat tcatagtggt aatgctgaac
      961 taatgcacat gataccacta ttattattgg atgacggact actgttggta tattcccact
     1021 ggcgaaaaat ggtaacaaat agaaagttgg tgccaatcgg caaaaaccga gcgctgtagc
     1081 agccagtaga ctatgcaact gatagagtat cacctccccc atgctatcct ctcgcaaatg
     1141 ctaaattcag catttcaata ccaaaagaat ataatttttc accataccat ccgctcatta
     1201 aaaaaaagca aatacttacg cacaataatt tcacgccaaa tggcaacgtc tgttcttgca
     1261 actgcgttac cgtttgaaaa agccctacca ataaaccaac aaatgtcgct actgcaatag
     1321 gtcctgcaga cataactaaa atcagataca gcgccctatt ccctgcaaaa acaatgtcat
     1381 ccatattttt ttcctcaggg attaatggat aggtcaaaat attgtaatat gagtcctttt
     1441 gataacattg tccaaccgtc catcgcgaca aacagaatta attttatcgg tgtagatatg
     1501 gttaccggac tcatcatcat catacctaac gttaacagaa cgctggatat aaccagatcc
     1561 actacaacaa agggtaaata gatataaaaa cctataataa atgcactttt tatctcactt
     1621 aacgcatatg ccgggagtaa cgagaaaatt gaaatattat catcatcgat tatctcttca
     1681 ttgtcttcag aactattcac cttctgaatc ttttcaaaaa agctaaccaa ctccggttca
     1741 gaatatttta tcaaatatga tttataacca ctcatgcctg tctcaacaaa attaacaacc
     1801 gacgctacat tattgaatga aagattctca ttctgactgt tataatatat ttccttccca
     1861 accggcatca ttacaaacat tgacaatagc aaagcaaccc cattaagtgt catatttgat
     1921 ggcacttgct gaagtcccaa tgcgtttcgg acaataacaa aaacaatcga gaatttaata
     1981 aaacatgtcc cggatgctat tataaacggc aatagtgtaa ataatgatag tatcgctatc
     2041 agcgaaatgc tatttgacat tattatgccc tgataaccaa gaatgaatct ctacgcccaa
     2101 tttatcatcc acttcaacaa gttcgccata gccagttagc gcgccattta cacgtatctc
     2161 tatatttttc tcagattcag gaagcagaga tattattctt tttgcacaga gctcgtctat
     2221 ttcatataga ttcattattt tcttaccaag aacaaactca atttttacag gcaactcttc
     2281 aaaactatta atcatctgtt cgtcattttc atcataaatc tcactctcgc tttttttgat
     2341 atcaaagtca tctgtttcaa aatcttcctc gtactgaaaa tgatcagcca tttttaactc
     2401 ctctggataa attaaatcac aaatctttgt attataaata accgcataag ccatattatt
     2461 tgagattaat aaaacatcac cgacttcaat atcaacaata gttct
//
LOCUS       DABSYT020000255         2460 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.257, whole genome shotgun sequence.
ACCESSION   DABSYT020000255 DABSYT020000000
VERSION     DABSYT020000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.257"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            1149..1865
                     /gene="nanC"
                     /locus_tag="ID725_RS25610"
                     /old_locus_tag="pgaptmp_005122"
     CDS             1149..1865
                     /gene="nanC"
                     /locus_tag="ID725_RS25610"
                     /old_locus_tag="pgaptmp_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGV7853506.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1885..>2460
                     /gene="nanM"
                     /locus_tag="ID725_RS25615"
                     /old_locus_tag="pgaptmp_005123"
     CDS             1885..>2460
                     /gene="nanM"
                     /locus_tag="ID725_RS25615"
                     /old_locus_tag="pgaptmp_005123"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGV7853507.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSF"
BASE COUNT          791 a          485 c          499 g          685 t
ORIGIN      
        1 taaaatcaac ataaaaaatt cagcaaaatt taatattcgt tcaactagcc gtctcacata
       61 tgacattatt agtcaactcc atttgccaca aaatggctat ttcatggaac acagtaattt
      121 cttagtggct attatcaagc taattcttag gtccctagcg attattcctg gctgattcgt
      181 cagcttacgc catgagattg ttacattcct ttttatcctg gagatatggg ggaacgcagg
      241 agatgatttt ttcatctaca ttcgttggta aatatagctt tggtcactac gacttaaacc
      301 ttcctcaatc acagccattc gccgggcgat acggcaaaca aactaagtga aacatcatcc
      361 tgaaaacacc aacatcaata agcctctcca gatcgacttc agaagtgacc agttacaagc
      421 cacaaaccag gaaacatatt tcatgatgag aattatgctc agcctgatgg cgggtaaaca
      481 ctgatttaca ccaagctcac tgagttagct tgcaaagctt cccttgttta gactctaaat
      541 aaattattta taaaacaaat agttaaacga agatctttag tttttgaaga ccaccgcaag
      601 tgttcgtctg gcttcacatg gcatcttctt cttagaaaaa gatcgacata ttttgtgaca
      661 cgaattgcaa atctggtttt gttgtatgga ttgcgtgatt ttcgatctgg tataacaggt
      721 ataaaggtgc accacgatag acaatgagac agggcatctt gcaatctatg gcaaacatca
      781 cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag cctgaaagga atatgaaaat
      841 gagatcaaca gacactctat tttatgactc tgggtaaaat gaattgagta agtgatatag
      901 cttacgaaca ttcaaatcaa ttaaacatca gaatagattt tatactcagg tatttaatct
      961 ggctctctgt ttatttaaat aatgtggaaa gagatttttc acaggagaca ttatacaaaa
     1021 aatataaaat acagctaccg gttgccaaag acactataag cctggcaaaa aaatattaca
     1081 caacataaat gctaattgtt tatgcgggct ttgtattgct ttctgtatcc tacaaatgag
     1141 tgaaatttat gaaaaaggct aaaatacttt ctggcgtatt attactgtgc ttttcgtcac
     1201 cattaatttc tcaggctgcg acactggacg tacgtggtgg atatcgtagt ggaagccacg
     1261 cctatgagac tcgactcaaa gtcagtgagg gatggcaaaa tggatggtgg gcaagcatgg
     1321 aaagtaatac ctggaatacc attcatgata ataaaaagga aaatgccgca ctcaatgatg
     1381 ttcaggttga agttaattac gcgattaaac ttgatgatca atggacggtg cgcccgggaa
     1441 tgttaacgca ttttagcagc aacggcacac gctacggacc ctacgtaaaa ctgtcctggg
     1501 acgcgacaaa agatcttaat tttggcattc gctatcgtta cgactggaaa gcttaccgac
     1561 aacaagactt atccggtgat atgtctcgtg ataacgttca tcgttgggat ggatatgtca
     1621 cttaccatat taatagtgat ttcaccttcg catggcaaac gacgctatac agcaaacaga
     1681 acgattatcg ctatgcaaac cataagaaat gggcgacgga aaatgcattt gttctacaat
     1741 accatatgac gcccgatatt acgccataca tagaatatga ctacctggac cgtcagggtg
     1801 tttacaacgg cagagataat ttatcggaaa acagttatcg cattggtgtg tcatttaaac
     1861 tgtagtagac aggagacagt cacaatgaat aaaacaataa cggcgcttgc tatcctaatg
     1921 gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc ctgtaccatt taaaagtggt
     1981 accggagcaa ttgataacga cactgtctac attggtttag gtagcgcagg tacggcatgg
     2041 tacaagctgg agacacaggc caaagataaa aaatggacag cgttagctgc attccctggt
     2101 ggaccaagag atcaagcaac ctcggcattt attgatggca atctgtatgt gtttggcggc
     2161 attggcaaaa acagcgaggg cttgactcag gtatttaatg acgtacacaa atacaacccc
     2221 aaaaccaata gctgggttaa attgatatcg cacgcgccga tgggcatggc gggccatgtg
     2281 acttttgtac acaacggcaa ggcttatgtt actggcggtg ttaaccagaa tatcttcaat
     2341 ggctattttg aagatctcaa cgaagctgga aaagattcaa ccgctgtaga taaaatcaat
     2401 gcacactatt ttgacaaaaa agcagaagat tatttcttta ataagtttct gttgtctttt
//
LOCUS       DABSYT020000256         2456 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.258, whole genome shotgun sequence.
ACCESSION   DABSYT020000256 DABSYT020000000
VERSION     DABSYT020000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.258"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            412..487
                     /locus_tag="ID725_RS25620"
                     /old_locus_tag="pgaptmp_005124"
     tRNA            412..487
                     /locus_tag="ID725_RS25620"
                     /old_locus_tag="pgaptmp_005124"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:445..447,aa:Ile,seq:cat)
     gene            1239..>2456
                     /locus_tag="ID725_RS25625"
                     /old_locus_tag="pgaptmp_005125"
     CDS             1239..>2456
                     /locus_tag="ID725_RS25625"
                     /old_locus_tag="pgaptmp_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HGV7853508.1"
                     /translation="MLINKSNGFNASAVWGSGSYNENKSSKHMELLAHSIVKLICKEA
                     ASETYRGALEILQKIMSECIYQEGNAFVIMGAGEQLKRIKYDVDENNLKVFNVHFDNN
                     EVLVTDGEPDVVCLSKQVWENLLIKLKPEIKENVASEVHKSANKGEIEQLVEWSKRNE
                     QTLFDNIIKSDFHVGSLKPGSMNGVILEMPPNVCMEPRNSYENKIDEVSSLSESEEHP
                     IDIQKITDAFVKEFKGILFDKNGRSSELLFNFYECCYTFLPRAQPQDKIDSYNSALQA
                     FSIFCSSTLTHNNVGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEG
                     GLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGMSLFDVDTPCIFNTPANHESYEKSLKP
                     VSENGLNGVLSDRN"
BASE COUNT          826 a          374 c          499 g          757 t
ORIGIN      
        1 cgatgtggtc tggagaggca actgtcaaaa atcgctcata cgaaactaat tattagcaga
       61 ataagttcta taaaagaata tattgctata tgaagtttat ttttatggag tgaaaagtaa
      121 cagatatcat aatgatgcga aaactcttca gttaatgtaa taaacttata ttattagata
      181 caaaatcatt acaaagtatc attgaatcga tgataactat agattgtgct aattattaac
      241 atttgcttgt tgactgcgag tatcgacttt gcggaatgga ggtggtgtcg ggttttatgt
      301 cgcatgtatt gacgacccag gatggatgag aacgcataaa aatctgtgat ttacatcata
      361 agatcatcat gtttattgcg tgggttataa gtattgttat tgcatactta aggcccttta
      421 gctcagtggt tagagcaggc gactcataat cgtccggtcg ctggttcaag tccagcaagg
      481 gccaccaaat catcattcat gtatcaggaa gagttaaggg tacaagggtt actcgatggc
      541 ggtctatatt tcacgattaa cgccatttca aggaagaagt gcggtgggtg gtttcgaatg
      601 agggctggtg ttttggtacg tggcacgcca ctgcaatgta tttatctatc agtactcatt
      661 caaaagtatt tggaaaaaat gcctgctatg ccaagcgggc aaattgctta catgtggaat
      721 taagtaatta acgataaagc cagcgtatta tcatttatta tacccattca tttaatcagg
      781 ttaattactc aaatcactga actattctct gttcgctcac tgatgataag ggagtaccac
      841 aaccaatgta tgtagaacaa tgccgtagag taaggatgaa atgtctctgc cacgccttaa
      901 acatctacta ctgactagaa cacccgccgc aggtagatga gttttgtgag cgttacagga
      961 gcgtggctat gctcattaat caacgctatt tagatataaa tttctaacac tcgaaaaacg
     1021 attttcacta ataaattata acgggtggtt tttatcatac agcgaaataa ccttttgttt
     1081 attaaattta acattcctgt gctaatttaa ttaaaagttc catgcgtagc aagtgaagca
     1141 gttaatgtca gcaattgtct caaatgagcg attttttata gtgacaaaca ataataacat
     1201 taaaaatagc catgtagaca ttaactgagg aatagaaaat gttaattaat aaaagcaacg
     1261 gatttaacgc tagcgcagtt tggggtagtg gaagttataa tgaaaataaa tcttctaaac
     1321 acatggagct actagctcat agtattgtaa aattaatttg taaggaagct gcatcagaga
     1381 cgtatcgcgg tgctcttgaa attttacaaa aaataatgtc tgaatgtata tatcaagaag
     1441 gcaacgcctt tgtcattatg ggagctgggg aacaattaaa acgtattaaa tatgatgttg
     1501 atgaaaataa cttaaaggta ttcaacgtac actttgataa taatgaagtg ttagttactg
     1561 atggtgagcc tgacgtagta tgtttaagca agcaggtctg ggaaaatctt ctcattaaat
     1621 taaaaccgga gatcaaggaa aatgtggctt ctgaagttca taaatcagcg aataaaggtg
     1681 agattgagca attagttgaa tggtctaaaa gaaatgaaca aacccttttc gataatataa
     1741 taaaaagtga ttttcatgtt ggttcactta agccaggtag tatgaatggt gttattttag
     1801 aaatgccacc aaatgtctgt atggaaccac gtaattcata tgaaaacaaa atagatgagg
     1861 tttcatcttt gtcagagtca gaggaacacc ccatagatat tcaaaaaata acagatgcgt
     1921 ttgtgaagga gttcaagggg atattatttg ataaaaatgg aagatcttca gagcttctgt
     1981 ttaattttta tgaatgttgc tatacgtttt taccaagagc gcagcctcaa gataaaatcg
     2041 atagctataa ttcagcactg caagcttttt ccatcttttg ttcatctacg ttgacacata
     2101 ataatgtagg ctttgatttc aaattatttc cagaagtcaa gctgtctgga gaacatcttg
     2161 aaacggtatt caaatacaaa aatggcgatg atgtccggga gatagccaaa attaacatta
     2221 ctctccaaaa agaagagggt ggcttatata atctacgtgg attggatttt aagggatgct
     2281 tcttttctgg acagaacttc agtaactatg atattcaata tgtgaactgg ggaatgtcat
     2341 tgtttgatgt tgatactccg tgtattttta atacgcctgc taaccatgag agttatgaaa
     2401 aatcattaaa acctgtaagc gaaaacggtt taaatggagt cttatctgat cgtaat
//
LOCUS       DABSYT020000257         2443 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.259, whole genome shotgun sequence.
ACCESSION   DABSYT020000257 DABSYT020000000
VERSION     DABSYT020000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.259"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..98)
                     /locus_tag="ID725_RS25630"
                     /old_locus_tag="pgaptmp_005126"
     CDS             complement(<1..98)
                     /locus_tag="ID725_RS25630"
                     /old_locus_tag="pgaptmp_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853509.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSD"
     gene            complement(82..1833)
                     /locus_tag="ID725_RS25635"
                     /old_locus_tag="pgaptmp_005127"
                     /pseudo
     CDS             complement(82..1833)
                     /locus_tag="ID725_RS25635"
                     /old_locus_tag="pgaptmp_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(1845..>2443)
                     /locus_tag="ID725_RS25640"
                     /old_locus_tag="pgaptmp_005128"
     CDS             complement(1845..>2443)
                     /locus_tag="ID725_RS25640"
                     /old_locus_tag="pgaptmp_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGV7853510.1"
                     /translation="AEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYA
                     ISEKSHGREEIRLHIVCEVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPKMTVR
                     YYISSADLTAGKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIA
                     INILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          491 a          723 c          555 g          674 t
ORIGIN      
        1 atgtcagaat ttgaattgtt atcaaaccca aggtaaacaa caaataaaca aagggtaatt
       61 aaaaaaatga caagtttgcc tctaataatt tgaagcatct gaactccctt gctttgcttt
      121 tattggatta tctgctttta taccatctaa cccgaagagt tgcccaatgt tatatttttc
      181 aatacatgca tcgctacatt tggcagaaaa atcgccacaa gcaataccga cagtgttatt
      241 tttaagatcc atgattcttt ctttagctgg ctgaccttgt ctatccccag ccgcctcatg
      301 atttttacct atcacgtcag ctattgttga tccaaatgtc ttagtcattc gacacatcaa
      361 gtaacaatat ctcattgcat cggcggcccc attatgagct ccgccaacaa tagcatccat
      421 atcataaccg aactggtaag cactgttagt aatgctgttt gcaacaatca atccaggtac
      481 accagatacg gcataaatta gtccacctga tgtcatattc tcaatcgcat ctaaccccat
      541 tgggtctatc acttgtatgg gattcaacgg atactgatac atattccatc cacctctcaa
      601 cccaatcggg tccggggtga tataccgccc ctgcaacgga tcgtagtacc ggtgacggtt
      661 atagtacagc cccgactcct catcatgctg ctggcccggc agacggtacg gctggtgcag
      721 gtgatgcggg ttctcctcat taagctggtt gccccattca tcatattccg cgctccacgc
      781 cgtattgccg tcttcgctga taagcgccag cggcagtccc cggtggtcac agtggtacag
      841 atgcagcgtg cgcgccggcg tgtattccgg ctccacctgt ttttccagtt gctccaccgt
      901 cagtccgcac tgcgcaagcc acgcccggct ttcactgctc acgcggtctg cccggatttc
      961 ttcctccagc ctgtccagca tcctcaccag ttctgccggg aacaccacac cgtgaccgtc
     1021 ctcactccct tcctgctgga gtttctccgc caggctgcgg cgctgcgctt tctcccgctc
     1081 gccgttctcc gtttcgatgc ggatgagcgg tgtgaagctc cccggccggt ataccgtctg
     1141 gatacgtgtg gtgccggtct gtaccgtcgt cagcctgtcg ccgtcccacc cgtaccaggt
     1201 cacctccggt ttacgcgaca gcgacatcca ccccgtcagg tcacgctccc gccgccacac
     1261 ccgtttcccc gtccggcggc ccagcgggtc atagaggtag cggctctcga ccagcggctc
     1321 gccgtattgt atccgcgtgt agaacaccag gcggtgctgg ttgtcgtaat ggtagtggtg
     1381 ggtccgctcg tcgtgcatcc ggataacccc ttccggaatg cggtccgtct tctccgtcag
     1441 cctgccgtat tcatcgtagc ggtagacata gtgcgcatct tccgcgatgc ggttatccgg
     1501 ccacgccgtg agcgtgctgt ccggatgcag ttccgggtcc ggcagccggt ttcctgccgg
     1561 gtccgttgca tacgggatat cgatatccag gttcgctgcc gtggtgtgga cgcccgtcag
     1621 ccttcccgtg tcgctgtaac ggtactcccg gctctcctgc gggccgctga tgcggattag
     1681 ctgtccgttg tcgttccagt cgtagtcaca gtcaagctgc gggaggttca ggtgcctgct
     1741 ctgtagctgc cctgtgaggg tgtacgccgt gacctgttcg taccccgctg tactgcccgc
     1801 cccgccgaag ctgcgggccg tctcacggtg cagccagggc aagattacga aagcccgctc
     1861 cccgcaagga ctgacgccag atagtttctg tccatggctg cttttcgcat cttacgtctt
     1921 aaccctgcct tgaatacctt atcattcgtc aaaatattaa tagcaatgtg ccgtatccct
     1981 gaaaataatt ctgctgcatt tcctcttctt attttgcagt cgtcttcatt cattaccacg
     2041 tccagacgcc agtgcagctt attctccacg tgccagtggt ttcggattgc tgtggcgaac
     2101 ttccctgcgg ttaaatcagc agaactgata taatatctga ccgtcatttt tggctctttc
     2161 ttttgttctg ctattatgga ccgaaaggag actgccacgc ataatttctt cagccctttc
     2221 cattcaaacg tgaaatcaat aagttcatca gggacttcgc aaacaatatg aagacggatt
     2281 tcttctctgc cgtgactctt ttcactaatt gcgtaactgt catgctctgg attatttaat
     2341 tctttcagcg gaaatttttc ctcaaaggct ttatttagcc gcccctggtt tccttttacc
     2401 gcgaataaat aatcacctcc ctgtttttgt atcttctctg caa
//
LOCUS       DABSYT020000258         2438 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.260, whole genome shotgun sequence.
ACCESSION   DABSYT020000258 DABSYT020000000
VERSION     DABSYT020000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.260"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            1..211
                     /locus_tag="ID725_RS25645"
                     /old_locus_tag="pgaptmp_005129"
                     /pseudo
     CDS             1..211
                     /locus_tag="ID725_RS25645"
                     /old_locus_tag="pgaptmp_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            207..1508
                     /locus_tag="ID725_RS25650"
                     /old_locus_tag="pgaptmp_005130"
                     /pseudo
     CDS             207..1508
                     /locus_tag="ID725_RS25650"
                     /old_locus_tag="pgaptmp_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
     gene            1656..1838
                     /locus_tag="ID725_RS25655"
                     /old_locus_tag="pgaptmp_005131"
     CDS             1656..1838
                     /locus_tag="ID725_RS25655"
                     /old_locus_tag="pgaptmp_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGV7853511.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            1876..2145
                     /locus_tag="ID725_RS25660"
                     /old_locus_tag="pgaptmp_005132"
     CDS             1876..2145
                     /locus_tag="ID725_RS25660"
                     /old_locus_tag="pgaptmp_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGV7853512.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            2221..2436
                     /locus_tag="ID725_RS25665"
                     /old_locus_tag="pgaptmp_005133"
     CDS             2221..2436
                     /locus_tag="ID725_RS25665"
                     /old_locus_tag="pgaptmp_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGV7853513.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          620 a          558 c          731 g          529 t
ORIGIN      
        1 ttacccgccg acggtgaaca cctactggcg acgtcgtggc agcacatatt ttgtatcaaa
       61 agccggtgag cgttatcgcc gggctgtggc gcttattgtt cgccagcagc ggctgaaatt
      121 aagcctgtcc ggaaggctgg cgataaagat tattgccgag ccaccggata agcgccgccg
      181 tgacctggac aatattctga aagcgccgct ggggtgtggg caggccgctg tataaggatc
      241 tcatcggtcg tacaaaagcc gcgctggcaa agaacccgaa aaatgtgctg cttgccgtgg
      301 tgtggatgca gggggaattt gactttgacg gaacgccagc aaatcacaca gcccgtttta
      361 cagaagtagt ggaacaatat cgtacggacc ttgcagatat ggtgggacag tgcgctggtg
      421 gttctgctga cggtgttccc tggatatgtg gagacacaac ttatttctgg aagcagaaga
      481 gcgaatccac ttaccagacg gtgtacggca gttacaaaaa caaaacggaa aagaatattc
      541 actttgtgcc gttcatgacc gatgagaacg gagcaaatgt cccgacgaac aaaccggaag
      601 aagacccgga tattccggca tcaggatatt acggtgcggc ctcccggacg tcggcaaact
      661 ggacgtcagc agaccgtgcg agccatttca gctcatgggc acgcaggggg attatttctg
      721 accgtcttgc ctcagcgatt cttctccatg caggacggac ggctgaactg gtgggtgggg
      781 aacaggttgt gatgccgccg gatgagaagc cgtcaccgga cacaccatca acaccgtcaa
      841 cggacgggaa atcagtgaca acgctgcttt attaccgtgc aacagagtca ggtggtttac
      901 tgaatccgca gggatgggga gctgaaggag ggcgtgcatt ggtagttgat gatgcaggtg
      961 ctgcaggagg taaggcgctg aggtggacca aacagacagg aagttcctcg tggtttatgc
     1021 agcatgatgc cggtaatggc gcagacctgc tggagaaggg cgggcttatc agttgtcgtt
     1081 ttaaagttga tggcacactg acagctaatc agtacgcact ggcgctgtac tggccggttt
     1141 cttcactgcc tcagggtgtc acactggaag gtaatgccgg tcataacctg ctggcgtcgt
     1201 tttacgtaca gagcgatgcc acagacctta atgtgatgta ccacaaggga aatgctggtc
     1261 agaacacgaa gctggggtca ttcggcgcat ttgataacga atggcatacg ctgggcttcc
     1321 gttttgccgg taacaacagt attgaggtga cgccggtcat tgatggtaag gacgggacgc
     1381 cgttcatgct gtcacagtca ccggtcggca cgtttacggc agacaaattg cgcgtgaccg
     1441 atatcactag cggtgcgaca tatccggtgc tgattgaaag tataacagtg gaagtgaata
     1501 acccgtaagc aggaaaaaaa ggccgccggg gcagggaaaa caaggagcca gaaccggcgg
     1561 caaatgtcgt tatatccaaa gcaaaacatg caggacactt ttttaaccaa caggtattaa
     1621 cgatgtcaac accatatcaa taaccgggag ggataatgag atttgtacag cttattttat
     1681 tgtatttctg cacggtggtg tgcacgttat atctggtaag tggcgggtat aaggttatcc
     1741 ggaactatat acgcaaaaag attgatgccg cggcggcgga aaaaatcagc gccagccagt
     1801 cagccggaac aaaacccgaa gagcctctca tttcgtagca actttcttaa caacaccttt
     1861 caacgagaaa atcccatgtc agaaataaaa tctctggtca ctgctgaagc agtgaaggac
     1921 gtcctgcgct ctgaagaagt cagaagcgca ctgaaacagc aactccgcca gaatcttgag
     1981 gcgcgtcttg atgctgaagt ggatgccatt ctggatgagc tgctgggggg accggctgct
     2041 cctgagcctg aagacggcgc gggtgacagt gctgtttcag atggcgttgt gtctcagcct
     2101 gacggtagca gtgagcctca gcctggcggc gaaatgatga tgtaaccata cgcaggggct
     2161 gtcggtgtga gctgatgccc ctcctgttgt tgtgagcttc cggattgcgg gagacggggt
     2221 atgtaccaga tggaaaaaat cacaacgggt gtgtcataca ccacgtcagc ggtggggacg
     2281 ggatactggt tactgcagct gctggacaaa gtctctccgt cccagtgggt ggcgataggt
     2341 gtgctgggga gtctgctgtt tggcctgctg acgtatctga ctaacctgta tttcaaaatc
     2401 agagaggacc gtcgtaaggc ggcgcgggga gagtaaag
//
LOCUS       DABSYT020000259         2423 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.261, whole genome shotgun sequence.
ACCESSION   DABSYT020000259 DABSYT020000000
VERSION     DABSYT020000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.261"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..281
                     /locus_tag="ID725_RS25670"
                     /old_locus_tag="pgaptmp_005134"
     CDS             <1..281
                     /locus_tag="ID725_RS25670"
                     /old_locus_tag="pgaptmp_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGV7853514.1"
                     /translation="SSLTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGY
                     NGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNVGNFGGFLSINKLSQ"
     gene            complement(298..618)
                     /locus_tag="ID725_RS25675"
                     /old_locus_tag="pgaptmp_005135"
     CDS             complement(298..618)
                     /locus_tag="ID725_RS25675"
                     /old_locus_tag="pgaptmp_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGV7853515.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            726..899
                     /locus_tag="ID725_RS25680"
                     /old_locus_tag="pgaptmp_005136"
     CDS             726..899
                     /locus_tag="ID725_RS25680"
                     /old_locus_tag="pgaptmp_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGV7853516.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            970..1893
                     /locus_tag="ID725_RS25685"
                     /old_locus_tag="pgaptmp_005137"
     CDS             970..1893
                     /locus_tag="ID725_RS25685"
                     /old_locus_tag="pgaptmp_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGV7853517.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKSTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTR
                     DVDINEPDGPTNLSAWVRLKNKELPPSLHRY"
     gene            1948..>2423
                     /locus_tag="ID725_RS25690"
                     /old_locus_tag="pgaptmp_005138"
     CDS             1948..>2423
                     /locus_tag="ID725_RS25690"
                     /old_locus_tag="pgaptmp_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGV7853518.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPEAY
                     FRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTG"
BASE COUNT          616 a          550 c          627 g          630 t
ORIGIN      
        1 tgtcatcgct gacggccatg acggcctcgt ttgtgctggc cacaccgacc gagacggacg
       61 gggcgctgtt tcccggtcgc atcatgctgg cgaacacctg tatgtgggat taccggggag
      121 atgaatgcgg gtataacggt cctgcggtgg cggatgagtt cgacaacccc accacggata
      181 tccgtaagga cagatgcagc aagtgcatgc gcgggtgtga actgcgcagg aatgtcggca
      241 attttggcgg tttcctttcc attaataaac tttcgcagta atggattatg cccaccgtca
      301 ggtgggtttt ttatttagta gttctctcaa cttttcgttc tgctctctga acttttcctg
      361 tatttctttt tgcatggcga taacctgggc ttgaagttga actagcgcat caacatttag
      421 cggaaccgag acgctgttaa ttttatctgc tatttccccg agtgtatttt ctgcattcaa
      481 ggcatcttcc agtatctgaa caatctctga gttcatagag cgcccgtttc gtttggctcg
      541 ttcagctata gcatctcgca ttccgtcagg aaaacggagg ttgaacttgt cgtagtcttt
      601 tacttgtttt tcggccattg caaatctctc aaaaaaaatc atggtgccat attgctatac
      661 gatttcaatg gtggcattat ggccttcatg gcgtcacttt ggcaccaaat aaaggagatt
      721 agataatgca agatacactt ttcactgagc gcaaaaatat caaactcaac cttcgccttc
      781 catcacggct gaatgaagac cttcgccgcc tggcggaaat ggactgtata tctctgaact
      841 ctgcaattgt tcgtttgctg gcaaaaggtg ttagggaaga ggtggcgaat ggtcgctaaa
      901 aacagcgaag cctcaatggc tgcaaccatt gaggcttcta aattaccagt taaccacgag
      961 aaaactgata tgactagttt agcaattgca gatcgcacaa tcaatgttcc attccacgga
     1021 acaaatctct ttttggttgg aattaacaat gagccttatg ttcctatgaa gcctgttgtt
     1081 gaaggtatgg ggatggtttg ggctgctcaa tttgttaagt taaaacagag gtttgtcaaa
     1141 ggtatttcgg aaatcgaaat accttctgct ggcggtaaac agttaatgac atgtcttgcc
     1201 tttcgtaaat ttgcggcttg gctttcaagc attcaaccaa acaaagtccg ccctgaaatc
     1261 cgcgacaagg taatccagta tcaggaagag tgtgacgatg tgctctacga gtactggact
     1321 aaaggccatg tgattaaccc gcgcaaagct aaaaaggcat tgccggggaa aatcaccact
     1381 gaacagcagg aagccattaa acaactcgtc atgagtcgcg gtcagtctct gccaaaagaa
     1441 aaacaggcga aggcgatgat caccatgtgg tcgtcactga aatctcattt tgggtgttca
     1501 tacaaagaaa tcagcgagga gcagtttacc gaagctctgt cacttgctgc tcgcgttccg
     1561 cttgaaggag agttaatcgg caaacaagag aagagcacca acgagctttc cgcaaaagaa
     1621 gcaaacagcc ttgtatggtt atgggattat gctaaccgct cacaggcatt attccgcgaa
     1681 ctgtatccgg cgctaaaaca aattcaatcg aactattccg gcagatgcta cgactacggt
     1741 catgagtttt cgtatgttat cggaatggcg agagatgttt taatcaacca cacacgagat
     1801 gttgatatta atgagccaga cggaccaacg aacctttccg catgggtaag gcttaagaac
     1861 aaagagttgc cgccttcact gcatcgctac tgacaattga caacttaaca aacccagctt
     1921 cggctgggtt ttttatcagg agttctcatg ctctatagca atatattggc gcacgcccgg
     1981 cgatgtgcgc cagcggagtc gtgcggcttc gtggtgagaa cgccggaggg ggagcggtat
     2041 atcccttgtg tgaatatctc tgcagagccg gaggcgtatt ttcgtattgc accggaagac
     2101 tggctgcggg cagagatgca gggggagatt gtggcactgg tccacagtca tcccggtggt
     2161 ctgccctggc tgagcgaggc cgaccggcgg ctgcagataa aaagtgcact gtcctggtgg
     2221 ctggtctgcc ggggggacat tcataaattc cgctgtgtgc cacatctgac aggacggcgc
     2281 tttgagcacg gggtgacgga ctgttacacg ctgttccggg atgcatacca tctggcggga
     2341 attgatatgc cggattttga gcgtgaggat gactggtggc gtaacggtca gaacctttac
     2401 ctggacaata tggcggtcac cgg
//
LOCUS       DABSYT020000260         2394 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.262, whole genome shotgun sequence.
ACCESSION   DABSYT020000260 DABSYT020000000
VERSION     DABSYT020000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.262"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..186
                     /locus_tag="ID725_RS25695"
                     /old_locus_tag="pgaptmp_005139"
     CDS             <1..186
                     /locus_tag="ID725_RS25695"
                     /old_locus_tag="pgaptmp_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGV7853519.1"
                     /translation="ITPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENP
                     SPELLTMLIRELSGRGR"
     gene            183..533
                     /gene="tnpB"
                     /locus_tag="ID725_RS25700"
                     /old_locus_tag="pgaptmp_005140"
     CDS             183..533
                     /gene="tnpB"
                     /locus_tag="ID725_RS25700"
                     /old_locus_tag="pgaptmp_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGV7853520.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            564..2149
                     /locus_tag="ID725_RS25705"
                     /old_locus_tag="pgaptmp_005141"
                     /pseudo
     CDS             564..2149
                     /locus_tag="ID725_RS25705"
                     /old_locus_tag="pgaptmp_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2178..2321
                     /locus_tag="ID725_RS25710"
                     /old_locus_tag="pgaptmp_005142"
                     /pseudo
     CDS             2178..2321
                     /locus_tag="ID725_RS25710"
                     /old_locus_tag="pgaptmp_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          613 a          609 c          677 g          495 t
ORIGIN      
        1 ataacgccgg atgagcagaa agaacctgtg gcggccattg cgccgtcttt atccacttcc
       61 actcagacca gagtcagtgc cagttcctgc aaggtggaat tccgtcacgg taacatgacg
      121 ctggaaaatc catcgccaga gctgctcacg atgttaatcc gcgaactgag cgggagggga
      181 agatgatttc actcccatca ggtacccgta tctggctcgt tgccggcgtt accgatatgt
      241 gtaaatcctt caacggactg ggagaacagg tacaacatgt gctgaatgat aatcccttct
      301 ccggtcacct gtttatcttc cgtggccgac ggggggacac cgttaaaatt ctttgggctg
      361 atgctgatgg tctgtgcctg ttcaccaaac gcctggagga aggccagttt atctggcctg
      421 cggtgcgtga cggtaaggta tccattaccc gctcgcaact ggcaatgctc ctcgataagc
      481 tggactggcg tcagccaaaa acatcccgcc ttaacgccct gacaatgttg taaaaaacac
      541 ctggccgcat tataaaaacg gccatgagtc agaaatacct cattcgcatt gcagaactgg
      601 aaagccagct ccgtcagaaa gaccagcaac tgagtctggt tgaagagacg gaggccttcc
      661 tgcactggca cgtgccgaag aaaagatcga agaagatgaa cgggaaatag aacatctgcg
      721 ggctcagata gaaaaactgc gccggatgct gttcggtacc cgttctgaaa aactgcgtcg
      781 tgaagttgaa caggctgagg ccctgctgaa acaacgcgaa caggacagcg atcgttacag
      841 tgggcgggaa gacgatccac aggttccccg ccagttgcga cagtcgcgcc atcgtcgtcc
      901 gttaccggca caccttcccc gtgaaataca gcgtctggaa tctgaagaaa gctgttgccc
      961 ggagtgtggc ggtgagctgg attatctggg ggaagtcagt gcagaacaac tggaactggt
     1021 gagcagcgcc ctgaaagtga tccgcacaga acgggtaaaa aaagcgtgta caaaatgtga
     1081 ctgcatcgtt gaagcaccgg cgccgtcccg cccgatagag cgtggtatcg cgggccccgg
     1141 attacttgcc cgcgtgttaa cgggaaaata ctgcgaacac ctgccactgt atcgtcagag
     1201 tgaaatcttt gcccgacagg gtgtcgaact gagccgtgca ttactctcca actgggttga
     1261 cgcgtgctgc cagttaatga cgccgctgaa tgatgccctg taccgttatg tgatgaatac
     1321 ccgcaagctt cacactgacg acacaccggt aaaggtactg gcaccgggcc tgaaaaagac
     1381 gaaaacaggg cgcatctgga cgtatgtccg ggatgatcgc aatgcgggtt cgtcatctcc
     1441 tccggcggtc tggttcgcgt actcaccgaa ccggcagggg aaacacccgg agcaacacct
     1501 ccgccccttc cggggtatcc tgcaggcgga tgcgttcaca ggttatgaca ggctgttcag
     1561 tgcagaacgt gaaggtggtg cgctgacaga agttgcgtgc cgggcccatg cccggagaaa
     1621 aatccacgat gtatacatca gcagcaaaag tgcgacggca gaagaagccc tgaagcgaat
     1681 cagtgaactg tacgccatcg aggatgaaat acggggatta ccagagtcag agcgtcttgc
     1741 agtcagacag cagcgaagca aagcgttact gacgtcgctg catgaatgga tgatggagaa
     1801 gaatggcacg ctgtcgaaaa aatccagact gggcgaagcg ttcagctatg tactgaatca
     1861 gtgggacgcc ctctgttatt acagtgatga cggactggcg gagacggaca ataacacagc
     1921 ggaaagagcg cttcgtgcag tctgtcttgg aaagaaaaat tacgtgttct tcggtagcga
     1981 tcacggcggc gagcgtggtg cactgctata cgggctgatc ggcacctgcc gtctgaacgg
     2041 tatcgatccg gaagcgtatc tgcgccatat tctgagcgta ctgccggaat ggccctccaa
     2101 ccgggttgat gaactcctgc catggaacgt agtactcacc aataaataag cgtcaatacg
     2161 gtgctccgtt gacgcttacc ttctttcatt cgctgaaggt ggaatgtatc cacggggaac
     2221 gctttagcag ccgggaaata atgcgggaaa cggtgtttaa ttatatcgag tgtgattaca
     2281 atcgctggcg tcgtcacagt gcctgtggcg gtctcagccc ggactgtctt gcactgcaaa
     2341 tattcagaaa tgagtctatg cagttcacca gtaaaatcag ccatcagaga ggga
//
LOCUS       DABSYT020000261         2370 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.263, whole genome shotgun sequence.
ACCESSION   DABSYT020000261 DABSYT020000000
VERSION     DABSYT020000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.263"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..260
                     /gene="espL"
                     /locus_tag="ID725_RS25715"
                     /old_locus_tag="pgaptmp_005143"
     CDS             <1..260
                     /gene="espL"
                     /locus_tag="ID725_RS25715"
                     /old_locus_tag="pgaptmp_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE stator protein
                     EspL"
                     /protein_id="HGV7853521.1"
                     /translation="DLLNEVVRETLSNNNIVGAIKITKNPDIKLDPGEANNIQLINDA
                     NTPHNKIIIENEYIRITLDPLEQISILLNSFKDNYLSIIQE"
     gene            265..918
                     /gene="escR"
                     /locus_tag="ID725_RS25720"
                     /old_locus_tag="pgaptmp_005144"
     CDS             265..918
                     /gene="escR"
                     /locus_tag="ID725_RS25720"
                     /old_locus_tag="pgaptmp_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HGV7853522.1"
                     /translation="MSQLMTIGSQPIFLIIVFFLLSLLPIFVVIGTSFLKISIVLGIL
                     KNALGIQQVPPNMALTSVSLILTMFIMSPIILQINDNISQEPINYTDSDFFQKVDEKI
                     LSPYRGFLEKNTEKDNVEFFERAAQKKLGNETILKKDSLFILLPAFTMGQLEAAFKIG
                     FLLYLPFIAIDLIISNILLALGMMMVSPVTISIPFKILLFILVGGWQKLFEFLLVVN"
     gene            918..1187
                     /gene="escS"
                     /locus_tag="ID725_RS25725"
                     /old_locus_tag="pgaptmp_005145"
     CDS             918..1187
                     /gene="escS"
                     /locus_tag="ID725_RS25725"
                     /old_locus_tag="pgaptmp_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HGV7853523.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            1187..1963
                     /gene="escT"
                     /locus_tag="ID725_RS25730"
                     /old_locus_tag="pgaptmp_005146"
     CDS             1187..1963
                     /gene="escT"
                     /locus_tag="ID725_RS25730"
                     /old_locus_tag="pgaptmp_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HGV7853524.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALIDFLFSLWDSFIKLMLSFSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            1956..>2370
                     /gene="escU"
                     /locus_tag="ID725_RS25735"
                     /old_locus_tag="pgaptmp_005147"
     CDS             1956..>2370
                     /gene="escU"
                     /locus_tag="ID725_RS25735"
                     /old_locus_tag="pgaptmp_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HGV7853525.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GMSFFVDIVGLVNTTIDSLNRPFLYAIREILGAVLNIFLLYILPISLIVFVGTVTTGV
                     SQIGFIFAVEKIKPSAQKISVKNNLKNIFSVKSIFE"
BASE COUNT          743 a          376 c          405 g          846 t
ORIGIN      
        1 atgatttact taatgaagtt gtaagggaaa ctctatctaa taacaatatt gtcggagcta
       61 ttaagatcac taaaaaccca gacattaaac ttgatcctgg cgaggccaac aatatacagc
      121 ttattaatga tgcaaacaca ccgcataata aaattataat agaaaatgaa tatattagaa
      181 taactttaga cccattagag caaataagta ttttattgaa ttcatttaaa gataattatc
      241 ttagcattat tcaggaatga tgatatgtct caattaatga ccattggctc acagccaata
      301 tttttaatta ttgtattttt tctgttatca ttactgccaa tatttgttgt tattggtact
      361 tcattcctga aaatttctat tgtactgggg atattgaaga acgcattagg cattcaacag
      421 gtaccaccaa acatggcgct aacatcagtg tctttgatac tgacaatgtt tattatgtct
      481 ccgataatat tacagataaa tgataatatt tctcaggaac caatcaatta taccgactct
      541 gatttttttc aaaaagttga tgagaaaata ttatcaccat atcgcggttt tttagaaaaa
      601 aatacagaga aagacaatgt agagtttttt gaacgtgcag ctcaaaaaaa attgggtaat
      661 gaaactatct taaaaaaaga ctctctattt atactgttac cggctttcac gatggggcag
      721 cttgaagctg cattcaagat aggttttttg ctctatttac cctttattgc gatagatttg
      781 atcatttcca atatcttatt ggccttgggt atgatgatgg tatcgccagt aacaatttcg
      841 attcctttta agatcttatt attcattctt gttggaggct ggcaaaaatt attcgaattt
      901 ctgttggtgg tgaattgatg gatactggat attttgttca attatgtgtg caaacgttct
      961 ggataatatt tatcctctca ttgcctacag tcatagcggc ctctgttatc ggtattatta
     1021 ttagtctggt ccaggctata acgcagttac aggatcaaac attgcctttt ttgctaaaaa
     1081 taatagcagt gtttgctacg cttgccctga cttatcactg gatgggaaca acaatcatca
     1141 acttctcgtc gataatattt gaaatgattc cgaaggtgaa cggctaatga atgagataat
     1201 gacggtcata gtatcatcat tttattgtat actcaggccg ctggggatgt ttattatcct
     1261 accaatattt tcaacaggtg ttttactcag taactttatt cgcaactcaa ttatgattgc
     1321 gtttacgctt ccgatcatag tagagaacta tacattttcg gaaaaattgc catctggaat
     1381 ctttcagtta acaggaatag cgctaaaaga aataagtatt ggttttttca ttgggttatc
     1441 atttactatt cttttttggg caatagatgc ggctggacag attattgata ctctaagagg
     1501 ctcaacaata tcttcaattt ttaacccgtc cataagtgat tcatcttcta tcactggcgt
     1561 tatcttgtac caatttatct ctgtgatctt tgttattcat ggtgggatac aaagcatcct
     1621 agataagcta tatttatcct acgagatatt accattacaa gccgatattg cattcaatcg
     1681 tgctttaata gattttttgt tttctctatg ggattcattt attaaactga tgttatcatt
     1741 ttcagttccc atgattatcg gtatattctt atgtgatatg gggtttgggt ttcttaacaa
     1801 aacagcacct cagctaaacg tattcacatt atcactgcca gtaaaaagtt tgatagcaat
     1861 ctttatattg ctattagtta ttcatgtttt tcctgatttt attacggcaa atattcattc
     1921 tgacataatt gatagatcgt taccgagcat gattaatgag tgaaaaaaca gaaaagccca
     1981 cacccaaaaa actaagggat ctaaaaaaga agggcgatgt aacaaaaagt gaagaggtaa
     2041 tggctgcagt gcagtcatta atcttatttt catttttttc tttatatggc atgagttttt
     2101 ttgttgatat agttggatta gttaatacga caatagactc gctaaataga ccgtttttgt
     2161 atgccattcg agaaatatta ggtgcggtat taaatatatt tttattatat attttgccaa
     2221 tttctttgat tgtctttgtt ggaactgtta cgactggtgt atcacaaata ggattcatct
     2281 ttgcggttga aaaaataaaa ccatcggctc agaagattag tgtaaaaaat aacctgaaaa
     2341 atattttttc tgtaaagagc atttttgagc
//
LOCUS       DABSYT020000262         2363 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.264, whole genome shotgun sequence.
ACCESSION   DABSYT020000262 DABSYT020000000
VERSION     DABSYT020000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.264"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            37..2193
                     /locus_tag="ID725_RS25740"
                     /old_locus_tag="pgaptmp_005148"
     CDS             37..2193
                     /locus_tag="ID725_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HGV7853526.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDSAIVTNCFGHI
                     IESQPPENYNPEYKVWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALTNLKDNRDFKGLYLKAL
                     ARSVADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFY
                     YTMTGVFLRGADVFRANWKPGEFAPLTDRKLLDKAWANGTAASLAGKPATVKAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDVTQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQVIDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGE
                     SEDNDDSAFELLCKIRTGETLTTKEVVVNEKKTTPPPLFTEASLLAALVRVADFVTDP
                     TIKKLLKDKDKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDA
                     LPDIAVNPDMTALWAEKQAAIENGDLTVEEFINELYSELSGMITDVDLGEMKIEAAAP
                     AGQSQRLNAPCPSCGKQIAIRPKGYFCTGCEFKIWKNLSGKDLSDKQVETLLTKGITG
                     ELKGFVSSRTNKEFSAKVKLIDKATGKLGFEFPPKK"
BASE COUNT          719 a          490 c          560 g          594 t
ORIGIN      
        1 tattgaaagt gcattagtta tttaaaggat aataaaatga gactttttat tgcagaaaaa
       61 cccgcagtag cgaatgatat tgttaaagcg cttggtggca attttactcg ccatgatggc
      121 tggtttgaaa gtgatagcgc aattgtgact aactgttttg gtcatattat cgaatcacag
      181 ccaccggaaa actataatcc tgaatataaa gtctggaaag ttgaaacgct tcctttacgt
      241 ctttatcccg tgaagtatca gcctgtcgaa agtgccgcaa aacaggttaa aacgattctc
      301 gaacttatca gacgtggaga cgtgactgaa attgttcacg ctggcgatcc tgatgatgag
      361 ggacagctac ttgttgatga agtcctggaa tatgcaggta acacaaaacc cgtaaagcgc
      421 gttctgatta acgacaacac gcttccggca gtgaaaaagg cactgacaaa tcttaaagac
      481 aatcgtgatt tcaaaggact ttaccttaag gcgctggcgc gttcagttgc cgatgccgtc
      541 tatggcttct ctatgacgcg tgcttacacc attccggcaa aagccagagg gtatcagggc
      601 gttctgtctg tcgggcgcgt tcagactccc gttcttggcc tcattgtgaa tcgtacccgt
      661 gctaaccaga accataaatc cagtttttac tacaccatga ccggagtctt tctgcgcggt
      721 gctgatgttt tcagggcaaa ctggaaacca ggggaatttg ctccgctgac agatcgtaaa
      781 ttgcttgata aggcatgggc aaacggaacg gcggcatctc ttgcgggaaa accagccacc
      841 gttaaagcgg cagcaactga tgataaaaaa acggctgcac cgttgccttt taacctggtc
      901 aggctccagc aatacatgaa caaaaagttc aaaatgacag cgcaaaaaac gttggatgtt
      961 acgcagcaac tacgcgaaaa atacaaagcg atcacttata accgctcgga ttgttcatat
     1021 ctttctgatg aacaattcag cgaagcgcca caggttatcg atgccctgaa atcagtcttt
     1081 cctcagtcgc tggatattga ttccgcacgt aaaagcaaag cgtttaacag tgcaaaggtg
     1141 actgcgcata ctgcgataat cccgaccgcc agtgtgcctg atgttaacgc actcagcacc
     1201 gacgagcgca atgtttacct ggcgattgca caacactatc ttgttcagtt catgcctgaa
     1261 aaagcatacc aggaagtatc ggttgccatt cagtgtggtg atgagtcgtt ctatgctcgt
     1321 gccagaaaaa caactgacag cggatttgag gcatttcttg gtgcggaaac cacagacgaa
     1381 ggtgaatcag aagataacga tgattccgct tttgaactgc tctgtaaaat tcgcacagga
     1441 gaaacactga cgacaaaaga agttgtagtt aatgagaaga aaacgacacc gccgccatta
     1501 tttactgaag cctccttgct tgctgcgctt gttcgtgtcg cggattttgt cactgaccca
     1561 acgattaaaa aattgttgaa ggataaagat aaagacaaaa aagatgaaca tggcggcatt
     1621 ggtacgccag ctacccgcgc agcgattctg gaaacgttga agaagagaaa ttatatcacg
     1681 ctggaaaaag gaaaactcat tccgacagat accggatatg cgcttattga tgctctgccg
     1741 gatatagctg ttaatccaga tatgacagca ttatgggctg aaaaacaggc ggctattgaa
     1801 aatggtgatc tgacggttga agagtttatt aatgagcttt acagtgaact gagcggcatg
     1861 attacagatg ttgacctggg cgagatgaag attgaagcag cagcgccagc aggccaatct
     1921 caacgcctga atgccccctg cccttcctgt ggtaagcaaa ttgctatcag gccaaaaggt
     1981 tatttctgta caggatgtga atttaaaatc tggaagaacc tctctggcaa ggatctttct
     2041 gacaagcaag tagaaacctt gctaacaaaa ggtattacag gggagctaaa ggggtttgtt
     2101 agttccagga cgaataaaga attttcggct aaagttaaat tgattgataa agcaaccgga
     2161 aaattagggt ttgaatttcc ccctaaaaag tagcaatggt tgattattcc ttgttctcca
     2221 tataacatcc aatggcacgt ctgaagcatc cgattaacca ggggtggttt gttagtcttc
     2281 agatggtgcc gttagcatga aaaggagaac atgatgaaaa tgaaactttt tgatgtgagg
     2341 aaataaagct aaggctgaaa aga
//
LOCUS       DABSYT020000263         2357 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.265, whole genome shotgun sequence.
ACCESSION   DABSYT020000263 DABSYT020000000
VERSION     DABSYT020000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.265"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            177..722
                     /locus_tag="ID725_RS25745"
                     /old_locus_tag="pgaptmp_005149"
     CDS             177..722
                     /locus_tag="ID725_RS25745"
                     /old_locus_tag="pgaptmp_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGV7853527.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            697..>2357
                     /locus_tag="ID725_RS25750"
                     /old_locus_tag="pgaptmp_005150"
     CDS             697..>2357
                     /locus_tag="ID725_RS25750"
                     /old_locus_tag="pgaptmp_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGV7853528.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL"
BASE COUNT          574 a          571 c          677 g          535 t
ORIGIN      
        1 gcggtttttc actatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgccg
       61 taacctaatg tttttattta aaacaccccc tgaaaagaaa ggaaacgaca ggtgctgaaa
      121 acgggctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg
      181 aagtcaacaa aaagcagctg gctgacattt tcggcgcgag tatccgtacc attcagaact
      241 ggcaggagca gggaatgccc gttctgcggg gtggtggcaa gggtaatgag gtactttatg
      301 actctgccgc cgccataaaa tggtatgccg aaagggatgc tgaaattgag aacgaaaagc
      361 tgcgccggga agttgaagaa ctgaggcagg acagcgagac agatctccag ccagggacta
      421 ttgagtacga acgccatcga cttacgcgtg cgcaggccga cgcacaggaa ctgaagaatg
      481 ccagagactc agctgaagtg gtggaaaccg cattctgtac tttcgtgctg tcgcggatcg
      541 caggtgaaat tgccagtatt ctcgacggga tccccctgtc ggtgcagcgg cgttttccgg
      601 aactggaaaa ccgacatgtt gatttcctga aacgggatat catcaaagcc atgaacaaag
      661 cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa cagtcaggtt
      721 aacaggctgc ggcattttgt ccgcgccggg cttcgctcac tgttcaggcc ggagccacag
      781 accgccgttg aatgggcgga tgccagttac tatctcccga aagaatccgc ataccaggaa
      841 gggcgctggg aaacactgcc ctttcagcgg gccatcatga atgcgatggg cagtgactac
      901 atccgcgagg tgaatgtggt gaagtctgcc cgtgttggtt attccaaaat gctgctgggt
      961 gtttatgcct acttcataga gcataagcag cgcaacaccc ttatctggtt gccgacggat
     1021 ggtgatgccg agaactttat gaaaacccac gttgagccga ctattcgtga tattccgtcg
     1081 ctgctggcgc tggccccgtg gtatggcaaa aagcaccggg ataacacgct caccatgaag
     1141 cgtttcacca atgggcgtgg cttctggtgc ctgggcggta aagcggcaaa aaactaccgt
     1201 gaaaagtcag tggatgtggc gggttatgat gaacttgctg cctttgatga ggatattgaa
     1261 caggaaggct ctccgacgtt cctgggcgat aagcgtattg aaggctcggt ctggccaaag
     1321 tccatccgtg gctccacgcc caaagtgaga ggcacctgcc agattgagcg tgcagccagt
     1381 gaatccccgc attttatgcg ttttcatgtt gcctgcccgc actgcgggga ggagcagtac
     1441 cttaaatttg gcgataaaga gacgccgttt ggcctcaaat ggacgccgga tgatccctcc
     1501 agcgtgtttt atctctgcga gcataatgcc tgcgtcatcc gtcagcagga gctggacttt
     1561 actgatgccc gttatatctg cgaaaagacc gggatctgga cccgtgatgg cattctctgg
     1621 ttttcgtcat ccggtgaaga gattgaaccg cctgacagtg tgacctttca catctggacg
     1681 gcgtacagcc cgttcaccac ctgggttcag attgtcaaag actggatgaa gacgaaaggg
     1741 gatacgggaa aacgtaaaac cttcgtgaac accacgctcg gtgagacatg ggaagcgaag
     1801 atcggcgaac gtccggatgc tgaagtgatg gcagagcgga aagagcatta ttcagcgtcc
     1861 gttcctgacc gtgtggctta cctgaccgcc ggtatcgact cccagctgga tcgctacgaa
     1921 atgcgcgtat ggggatgggg gccgggtgag gaaagctggc tgattgatcg gcagattatt
     1981 atgggccgcc acgacgatga acagacgctg ctgcgtgtgg atgaggccat caataaaacc
     2041 tatacccgcc ggaatggtgc agaaatgtcg gtatcccgta tctgctggga tactggcggg
     2101 attgacccga ctattgtgta tgaacgctcg aaaaagcatg ggctgttccg ggtgatcccc
     2161 attaaagggg catccgtcta cggaaagccg gtggccagca tgccacgtaa gcgaaacaaa
     2221 aacggggttt accttaccga aatcggtacg gataccgcga aagagcagat ttataaccgc
     2281 ttcacactga cgccggaagg ggatgaaccg cttcccggtg ccgttcactt cccgaataac
     2341 ccggatattt ttgatct
//
LOCUS       DABSYT020000264         2310 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.266, whole genome shotgun sequence.
ACCESSION   DABSYT020000264 DABSYT020000000
VERSION     DABSYT020000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.266"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1295)
                     /locus_tag="ID725_RS25755"
                     /old_locus_tag="pgaptmp_005151"
     CDS             complement(<1..1295)
                     /locus_tag="ID725_RS25755"
                     /old_locus_tag="pgaptmp_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGV7853529.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTE"
     gene            complement(1359..>2310)
                     /locus_tag="ID725_RS25760"
                     /old_locus_tag="pgaptmp_005152"
     CDS             complement(1359..>2310)
                     /locus_tag="ID725_RS25760"
                     /old_locus_tag="pgaptmp_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HGV7853530.1"
                     /translation="ARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNL
                     GYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRV
                     SLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVVRRKSE
                     ASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAE
                     AGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPKGNAIL
                     ITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT          488 a          762 c          581 g          479 t
ORIGIN      
        1 tcttccgtca gtaccgcaat ggccgacacc ttcgcatgac tgaaggtgat ggattcaaaa
       61 tcaaacttcg tcaggggttt tgccttaccc tcacccaccc agccggcagc accgccggac
      121 acctgggcgt gcacacggat attgaacggc acctgacgaa gtgcagggat cccgccctga
      181 ccaaatcgcc cgataatggt ctgcggacgc aggtaatcaa taaagtcctg tgcgtattcc
      241 tgatattcag acaggctgcc tgcccactgc ggatccgtgg tggtccccgc gcccactgcc
      301 gatttcagga catgatgcag acgactgtca tccggatact gacgacgggc cacttccagg
      361 gcttcagatc ggacgccttt agccgcagcc agcgatttgg caaagcgggc gaagccaatc
      421 cccttatcca gtttctgctc cacacggatc accggcgcag aagccaccgc ggccacattc
      481 ccgttaccgg cctgtttcac cggctgcgcc gtggcggcct taccggcttc cagttcacgc
      541 aggcgcttca ggtgcgcatc cacctgacgg atttccgctg cggtgttgtc gtaatgctct
      601 tcctcctcca catccagcgt gcgcccttcc tctgcggctt tggtcatgac ctcctcaagg
      661 gaggctgcca gcgctgcacg cttgttttca aaacttttaa tctgttcgcc aatattcatt
      721 atggtctttt ccttatgaaa aacggttgtt gactgtgccg cagcgccggc agaagatgcg
      781 attttcacca ccggtttccg gttgccggac gcggcagaaa acgggcggtc gtaagattta
      841 atggtccgga tggtgcattc cgcattcgcg ggcacggtga cggcagacac ctccatcagt
      901 tcccagcgca gaaaatgcag tccgcctccg tccagaaagg tgtattcatg gggacggaag
      961 cccacggaca gccccctgac cagcccggtc ttaatggccg cccagacctc atccagccgg
     1021 gcagccagtt gcgacggcat atccggtacg ggcttcacca gtgttgccgt gatttccagc
     1081 ccttcgctga cccggcgcac cgtacactgc cccaccgggc gggaatggtc atgctgccag
     1141 agaaacggga tcgcactgcc aaactccgcg ccctccggct ccaggatgtc accatcccga
     1201 tccggagaag gcgttgacgc aatcccggtg atcacccgtt catcctcact gaaggatttc
     1261 accgtcagca gggaacaggc ccgtttaaga gtcacatcag cctcctgaaa ataaaaaaac
     1321 cgccgcagcg gttcatgatg gttacagtgt gaacagggtt atatgaaaaa aaccgcatat
     1381 tctttctttt tcggttccgg gttaagggac atcagggaga ccgcattgaa cagcgccatc
     1441 agcgggtcaa tttttccccg tccactggcc tgtttggtga taagaatggc gttaccttta
     1501 ggctccaccc gggcattgcc gacacaccag gccatcaggg gctggtcacc atgcaccagc
     1561 accccttcag ccagtttgcg ctcggtggtt ttaatggccc cgcccagttt ccagccctgg
     1621 cttatcccca ccacaattcc gtcggggatc ccggcttccg ccagtgaatc cagaatctgc
     1681 cccacccctg acgggtcaat accgatatgg tccagtaact cagcctcatg aatgcgacgc
     1741 acatattccg ccacttccgc cgtgtcatcc ccgacacgcc ggacaatggt catatctcca
     1801 caggcaacaa gatcctgaaa ccgggacgcc tcgctcttcc gtcggaccac cgcggtttca
     1861 tgcgcccagg catggcccca gcccagccat tcgcgggttt ccctgtcacg gccaatcacg
     1921 tacattccca gcagatcatc caggcccccg ccgtcaatcc ccaccgtcac cacatcagcg
     1981 cgctgcagga tatcgtccag gctgacgcgc ctgccctgct gctcccagaa atccgcaccc
     2041 gcccagcggt cagaacgcag ggcaagacca atttccacat tggcatgttt tgacatgaag
     2101 ccacgaaatg cttcctcacc agcctcccgg gctttacggt actcccggta cagaaaagcc
     2161 tcatccaccg aataaccgag attcgggtta accatggcga ggttttccat cagcaggtga
     2221 gccccgcttt ccaccatttc aggaggatgc tcaaaaatca ccggcagaaa gtgcggatca
     2281 tgaattttgc cgtcacggac atcccgggcg
//
LOCUS       DABSYT020000265         2281 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.267, whole genome shotgun sequence.
ACCESSION   DABSYT020000265 DABSYT020000000
VERSION     DABSYT020000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.267"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(84..1250)
                     /gene="dacD"
                     /locus_tag="ID725_RS25765"
                     /old_locus_tag="pgaptmp_005153"
     CDS             complement(84..1250)
                     /gene="dacD"
                     /locus_tag="ID725_RS25765"
                     /old_locus_tag="pgaptmp_005153"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGV7853531.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
BASE COUNT          617 a          570 c          482 g          612 t
ORIGIN      
        1 agttgcaata ttaaaaccac aaggaatgag tgttgattat gcgagcagac tcgcactcct
       61 gccagtctgc tgcaaaagaa aggtcaggcc ttatggtgga aataatcact caggcgagaa
      121 aacatgctgc cttccccgac agattccagg gtaaccagcg gccagtgcgc cacctgttta
      181 tcacggtcgt aaagttcaat ttcccctacc cgctgatggg cgctaattgg cgcggtgagc
      241 tctttaccat caagggtata tttggctttg atatgtggaa tttcggcttt cggtagcacc
      301 atccagaact cttgttccgt tcccagggcg atattttctt tatcgccata ccagatgcgt
      361 tccgtaccga cctttttccc acggtgcaaa atttgcaccg tagtaaagtt ttgttgcccc
      421 caacgcagta attttcttgc ctcttcctca cgaccttttg cactgtcagc ccccattacc
      481 actgcaatga gacgacgctg cccatctaca gccgaagcaa tgagattaaa cccggcacca
      541 gaagtatgac ccgttttcag gccgtcaaca ttcatggttt tatcccacaa taacccgtta
      601 cggttttgct gggtgatacc gttccaggtg agacttttct cactgtacat atgataaaac
      661 tcgggctcgc cgtggatgat agcgcgagaa agcacagcta aatcataagc cgagctatgc
      721 tgacctggtg catccagacc atgcactgtt tcaaaatgcg tatccttgag atgcagcttc
      781 tcggcatagt tgttcatcat ttcaacaaac tgccgttgcc caccggcaat atagtcagcc
      841 agtgcaacac aagcgtcatt tccggaatcc acaattaaac cacggcttaa atcacgtacc
      901 gatacgcgat cgccctcttt caaaaacatc agtgaagaac cgacaaacac tggattatct
      961 ttcgcccacg catcgcgccc cacggtgaca atatcgtctg gcgtaatgcg atgactatcg
     1021 atagcgcgat ccacgacata gcccgtcatc agctttgtca ggctggcggg attgcgctgt
     1081 tgatgctcat tacccgcggt gaggatctga ccggtggtgt aatccatcaa tacccaggag
     1141 ccggcatgaa tctctggagg ctgaggtgaa aaaggaatgt tttccgccgc aaaaccagac
     1201 gataagttaa aaatgaacaa agaagcagca ataataagac ggcgtttcaa cagcaaaccc
     1261 tcaggagttt caaatagctg ttctttttac ggaaatactt atgaactggc tggaataaag
     1321 tgcaagaaaa tgtgactacc ctctcatttt tatctgacat gatctgttgc cactcgctgt
     1381 caaattgtcg cgctaaagct gattagcacg gtgatatttg atactctggc agacagcaga
     1441 aacaacggat ttaacctaat gatgaatgac ggtaagcaac aatctacctt tttgtttcac
     1501 gattacgcgc aacacccctg agaaccgcca tgaacaagtg tttcatgatt tgtgatttta
     1561 gtttggtacg caatttggta cacaacacaa ttttcacttc agtgggtagt catcaaactc
     1621 accataacga gcatcgttga tgatgtatgt tataaccaca aacatcacat ctggactagt
     1681 tccgttttca tgagcgggta tttgtttacg cctccctttc tgcaaatcct aaagatgaga
     1741 ttttggggtt ttgggatatc ttttatcctc agttctctat ccattatgca aaccagtaat
     1801 gaaccatcac cgtgtgatag tgaggcgtct gccacgataa gcgcagagaa atgatgcaat
     1861 ggtgggcgga ctggcttgat gaaaaggtag agtgagcgac cttaaactat cgaatagaac
     1921 aaagtcttgc aatccagtgc aaagctttgt gcgcctcagt tttgtctaag cgttctactg
     1981 aaaacatagt aaaatcggta acggctggaa atcattcaat actcgcacta tcgaaagttc
     2041 gccagccagc cgtggcacgt tcttgcatac gacgtgccgc ggatcctatt attctagtat
     2101 gaaatcccta accgatttca aattctccag actgctcaga ttctctttta tcccacgcta
     2161 gcgctgataa atctcgtcat tgcaatcgac ctgcgctatc gctgccgcag ttcttccagt
     2221 ttctgcatcg ataactccat cttctgagca ataaacctca cctatgcaat attttatgtc
     2281 a
//
LOCUS       DABSYT020000266         2278 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.268, whole genome shotgun sequence.
ACCESSION   DABSYT020000266 DABSYT020000000
VERSION     DABSYT020000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.268"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..1406
                     /locus_tag="ID725_RS25770"
                     /old_locus_tag="pgaptmp_005154"
     CDS             <1..1406
                     /locus_tag="ID725_RS25770"
                     /old_locus_tag="pgaptmp_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGV7853532.1"
                     /translation="MLHVHSFNKSKTKHSTLSMLKIVEFVLLKINMEANVNYCNNSIY
                     DECIRIASEKYSKAHAFAIGKELEKLSSFLNDNRMTNSFYLFWVNPIRYRITQSWTGY
                     DSSLEGHSRLPDIKSVIAIAEIFSKRDEQLSSRDIFTTSVLALLMCAPSRISEILALP
                     ADCEITECDGKGIQRYGLRFFSAKGYEGNIKWIPTLMIPVAKKAISRLKELSSQARLL
                     AAEIQKNYSNSTKGTLKENIPPDLFWYDREKKIKYSNALCLLTEGQLNQNKKEMSDKL
                     FRPTTNFFKTDIIDSDYIKGYFNVFKRHGYINEDGSPYLLRTHQLRHLLNTFAQINGM
                     DEFSIARWSGRKLISQNVSYDHRSHLQMSKAIREKKLSVCVNEHRIKDIPVVDLNEFD
                     SLSSGAVLVSKHGYCKHSYAFKPCDNYPIKNSGLDNETISNIHDKILKRTLYDKNDGN
                     INADKWYEFHKKIKKGE"
     gene            1407..1805
                     /locus_tag="ID725_RS25775"
                     /old_locus_tag="pgaptmp_005155"
     CDS             1407..1805
                     /locus_tag="ID725_RS25775"
                     /old_locus_tag="pgaptmp_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853533.1"
                     /translation="MAKHINGYHEKKIVDVINTWSIDEKLTWNALCDRLVRVIGKRPS
                     RQSLSSHVRIAESFNVKKTTIKSGVIHTVKPANLKVASQRIKRLEAENEALKALNERY
                     LEQFKVWLYNAHLKQITIEELNNPLPAKYL"
     gene            1903..>2278
                     /locus_tag="ID725_RS25780"
                     /old_locus_tag="pgaptmp_005156"
     CDS             1903..>2278
                     /locus_tag="ID725_RS25780"
                     /old_locus_tag="pgaptmp_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853534.1"
                     /translation="MDKTNINLMERYLLLLDRFVDKLAESGFSEQRIIEQSYLFCAGF
                     YIKYQSGIEKMTFSNREVVLTFLLLSYYSHINKLDDDLINKERMKHVCSSLINFIVSN
                     GSRTEKVYANEKRKYEASTLKRE"
BASE COUNT          831 a          323 c          418 g          706 t
ORIGIN      
        1 atatgttgca tgtgcattca ttcaataaat ctaagacgaa acactcaaca ttatcaatgt
       61 tgaagattgt cgaattcgtt ttacttaaaa tcaatatgga agctaatgta aactattgca
      121 acaattcaat ctatgatgaa tgcataagga tagcctctga aaagtattct aaagcacatg
      181 catttgctat tgggaaagaa cttgagaaat tgagttcatt tttaaatgat aataggatga
      241 ctaactcatt ttatttattt tgggtaaatc caattaggta taggattact cagtcttgga
      301 ctggttatga ttcttcactg gaaggccatt ctagattgcc tgatatcaaa tcagttattg
      361 cgattgctga gattttttca aagcgggatg aacaattatc gtcaagagat atatttacta
      421 catctgtgct tgctttactt atgtgtgcac cgagtaggat atcggaaatt ttagctttgc
      481 cagcggattg tgaaatcaca gaatgtgatg gaaagggcat tcaaagatac ggtttaagat
      541 ttttttcagc aaaagggtat gaaggcaata taaaatggat tccaacttta atgatacctg
      601 tagctaaaaa ggctattagc agattaaaag aattatcaag tcaagcgagg ttattggctg
      661 ctgaaattca aaagaattac tctaattcaa cgaagggaac ccttaaagaa aatatacctc
      721 ctgatctctt ttggtatgat agagagaaga aaatcaaata ttctaatgcg ctttgcttgt
      781 taactgaagg acagttaaat caaaataaaa aggaaatgtc agataaatta ttcagaccta
      841 caacgaattt ttttaaaact gatatcattg attctgatta tataaaaggg tattttaatg
      901 tttttaaaag acatggttat ataaatgaag atggtagccc atatttgcta agaacacatc
      961 aactaaggca tcttctcaac acatttgctc aaataaatgg tatggatgaa tttagtattg
     1021 ctcgctggtc tggacgtaag cttatttctc aaaatgtttc ttatgaccac agatcgcatc
     1081 ttcaaatgtc taaagcaata agagaaaaaa agttatcagt atgtgttaat gagcacagaa
     1141 taaaggatat tccagtagtg gatcttaatg agtttgactc acttagtagt ggtgcagtac
     1201 ttgtatcaaa acatggctac tgcaagcact catatgcgtt taagccgtgt gataattatc
     1261 caattaagaa ctctggttta gataacgaaa cgatttcaaa tatccacgat aaaattttaa
     1321 aaagaacact gtatgataaa aatgatggga acataaatgc tgataaatgg tatgaattcc
     1381 ataaaaaaat aaaaaaagga gaataaatgg ctaagcatat aaatggttat catgagaaga
     1441 agattgtaga tgttattaac acatggtcta tagatgaaaa actaacctgg aatgctttgt
     1501 gcgataggtt agtcagggtt attgggaaaa gaccatcaag acaatcctta agtagtcatg
     1561 taaggattgc ggaaagcttc aatgtgaaaa aaaccacaat caaatctggt gtaatacaca
     1621 ctgtaaaacc tgctaattta aaagtggctt ctcaacgaat taaaagactt gaagcagaaa
     1681 atgaagcttt gaaggcatta aatgaacgct atttagagca gttcaaagta tggctttata
     1741 atgctcacct taaacaaata acgattgaag aacttaataa tccgttacct gcaaaatacc
     1801 tttaataaaa ttgataggtt ttagctattg acatggtgaa atattatgtt atttattttc
     1861 ttgtcaggac gacaaaattt cactattgca aggatgcata ttatggacaa aacaaatatt
     1921 aacttaatgg aaaggtatct tcttctgctt gatcgattcg ttgataaact cgctgaatca
     1981 ggtttctcag agcaacgaat tatcgaacaa tcatatcttt tttgtgcagg attttatatc
     2041 aaataccaga gtggtataga aaaaatgacc ttctccaaca gggaggttgt tttaaccttt
     2101 ctgctacttt catattatag tcatataaat aaactggatg atgatttgat caacaaagag
     2161 cgtatgaagc atgtatgttc ttcattgatt aactttatag tcagtaatgg tagccgaaca
     2221 gagaaagtct atgctaatga gaaaaggaaa tatgaggctt caactctaaa aagagaat
//
LOCUS       DABSYT020000267         2269 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.269, whole genome shotgun sequence.
ACCESSION   DABSYT020000267 DABSYT020000000
VERSION     DABSYT020000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.269"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            146..352
                     /locus_tag="ID725_RS25785"
                     /old_locus_tag="pgaptmp_005157"
     CDS             146..352
                     /locus_tag="ID725_RS25785"
                     /old_locus_tag="pgaptmp_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGV7853535.1"
                     /translation="MEKITTGVSYTTSAVGTEYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(608..880)
                     /locus_tag="ID725_RS25790"
                     /old_locus_tag="pgaptmp_005158"
     CDS             complement(608..880)
                     /locus_tag="ID725_RS25790"
                     /old_locus_tag="pgaptmp_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853536.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1040..1573
                     /gene="rrrQ"
                     /locus_tag="ID725_RS25795"
                     /old_locus_tag="pgaptmp_005159"
     CDS             1040..1573
                     /gene="rrrQ"
                     /locus_tag="ID725_RS25795"
                     /old_locus_tag="pgaptmp_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGV7853537.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1794..1907
                     /locus_tag="ID725_RS25800"
                     /old_locus_tag="pgaptmp_005160"
     CDS             1794..1907
                     /locus_tag="ID725_RS25800"
                     /old_locus_tag="pgaptmp_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853538.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1909..2208
                     /locus_tag="ID725_RS25805"
                     /old_locus_tag="pgaptmp_005161"
                     /pseudo
     CDS             1909..2208
                     /locus_tag="ID725_RS25805"
                     /old_locus_tag="pgaptmp_005161"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2162..>2269
                     /locus_tag="ID725_RS25810"
                     /old_locus_tag="pgaptmp_005162"
     CDS             2162..>2269
                     /locus_tag="ID725_RS25810"
                     /old_locus_tag="pgaptmp_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HGV7853539.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPAPDW"
BASE COUNT          577 a          466 c          625 g          601 t
ORIGIN      
        1 ggccggaatg ttatctggca tttttagcag agcctgaatg ccataatcac ggctcccgga
       61 gttggccgtc agtgggtgac actggcggct tttttgtttt tctttacttt cattttctgt
      121 cggcggtgac ggagacatac atcagatgga aaaaatcaca acaggtgtgt catacaccac
      181 gtcagcggtg gggacggaat actggttact gcagctgctg gacaaagtct ctccgtccca
      241 gtgggtggca ataggtgtgc tgggaagtct gctgtttggc ctgctgacgt atctgactaa
      301 cctgtatttc aaaatcagag aggaccgtcg taaggtggcg cggggagagt agtcgatgaa
      361 taaacaatac gaactggttg taaaatgaat atttctaact gaaaaaacgt tccatgaggt
      421 aagaaaaggt cacaggcaat caataacagg acgtgatgaa agacccttgc atttgtgcgc
      481 tttctcttta gatagcagca gatactgaaa atctgagttg tcggggagtc agggatacag
      541 ctgtgcaaga gttggtcatt gtgattccat tgaaatcctg tatgccatga agggcaggat
      601 tttatggcta cctgagcttt ggtgatagta agttgaaaat tcgcattttt tgctgacatg
      661 cgtaacgaga atcccataag cagggaggac ttaattcttc attaacccat gcgttgatat
      721 tatgtttcag ccgttgaagc atcagcggtg ttaatgttgt ggtaataata tccagcgttt
      781 tatgtgagat cttaccgtaa gggtctgcaa gaatgctgct tgttgcttcg ttattatctg
      841 ccatcagaag aagtaactct gatttaacgt tttctgtcat tagttgtaaa aatcttctgc
      901 gcaaactttc tttactgttc atttatatgg cttcatttgt tgtaatctgc tgcgtctcaa
      961 gggatatgtt tatgagagcg accatgagtg ttggattata tacctaacat atcaagggat
     1021 tagaaatcga taaatcccca tgaacgaaaa aataaaatac ggcctgtcgg ctgccgttct
     1081 ggcgctgatt ggtgcaggtg cttctgcgcc tgaaatcctc gaccagtttc tggatgaaaa
     1141 ggaaggtaac cacaccacgg cataccgtga tggtgcgggg atctggacca tctgccgtgg
     1201 tgccattctg gtggatggta agcctgttat tcctggcatg aagctgtcaa aggaaaaatg
     1261 cgaccgggtt aatgccatcg aacgtgacaa ggcgctggca tgggtggaga aaaacatccg
     1321 ggtgccgctg accgaacccc agaaagcggg gatcgcgtca ttctgtccgt acaacattgg
     1381 ccccggtaag tgcttcccgt cgacgtttta taaacgaatt aatgcaggcg atcgaaaagg
     1441 tgcctgtgag gcgattcgct ggtggattaa ggacggtggc agagactgcc gtatccgttc
     1501 aaataattgc tacggtcagg tctcacggcg tgaccaggag agcgcgctgg cgtgctggga
     1561 catcgacaga tagcagaata ttttcctgaa aaatgacgtt ggccaacgcg ggtggataac
     1621 acgaaatcct gaaaactggt aaaacctaag tgaataaaag taaaaacccc gtttgttggc
     1681 agcaagcggg gttttgtgtt ttctgacctt gagtaaggca agggagaaat tatgggtagg
     1741 gaggtacttt ccctgtgagg aagtataaaa gattctttct gaggttgtcc attatgaaag
     1801 gcattgaagt ggagacgcca gccagtctgg atttaacaag agcggcagct tttgccattc
     1861 gtattgtggc cattgctgtt ctggtctggg caatccgttg gtggtgatat gaaccgtgtt
     1921 ctgtgtgtgg ttatcattgt cctgctggtg gcctgtggtg cgcttagtct ggggctgaat
     1981 cattaccgtg atcacgccat catctacaaa gagcagcgcg ataaaaaagc cagtgagctg
     2041 gagctggcga acgcgacaat tactgatatg cagatacgcc agcgtgatgt cgctgcactt
     2101 gatgccagat actcgaggga attagccgat gcgagagctg aaaatgaaac tctgcgtgct
     2161 gatgttgccg ctggtcgtaa gcgcctgcgg atcaacgcca cctgctccgg taccgtgcgt
     2221 gaagccaccg gcacctccgg cgtggataat gcaaccggcc cccgactgg
//
LOCUS       DABSYT020000268         2253 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.270, whole genome shotgun sequence.
ACCESSION   DABSYT020000268 DABSYT020000000
VERSION     DABSYT020000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.270"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..408)
                     /locus_tag="ID725_RS25815"
                     /old_locus_tag="pgaptmp_005163"
     CDS             complement(<1..408)
                     /locus_tag="ID725_RS25815"
                     /old_locus_tag="pgaptmp_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HGV7853540.1"
                     /translation="MNVVSEFITTINTVVQSGAASNAAKIAKAISPVFVAAVLLYIVY
                     VAYEITYSQRDIILSEVTKLIASLSLVGAFTYTAPYYSQYVIPFVMHAGSDLSNALTG
                     TGDVATSVDGLWEKLSTIMDNFISSKVQELSMTD"
     gene            741..1352
                     /locus_tag="ID725_RS25820"
                     /old_locus_tag="pgaptmp_005164"
     CDS             741..1352
                     /locus_tag="ID725_RS25820"
                     /old_locus_tag="pgaptmp_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGV7853541.1"
                     /translation="MILSTVALMMLFSQCAPTVSPVTLNAIVQTESSGNPYIVANISD
                     GISKSFSNKGSAVAYLNALAKSGKHFSAGLMQIYSKNFKALGLDNENVFDPCKNIKAG
                     AEILTKNYLAKKGGNEQENLLKSLSEYYSGNSTRGFKKEAQFNNTSYVERVMKNAYVV
                     PELRVDKPENAKATKEQSVQEKGNQSFVSHSQDWDVFGDYSNE"
     gene            1345..1638
                     /locus_tag="ID725_RS25825"
                     /old_locus_tag="pgaptmp_005165"
     CDS             1345..1638
                     /locus_tag="ID725_RS25825"
                     /old_locus_tag="pgaptmp_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HGV7853542.1"
                     /translation="MNKFANVNRFVFALFFMMFSGFAAAAGGLDSATNALTNVKTWLF
                     TFVGIGALVYMLYCILMAFMERKSWGDVGMALGYCALAGGAVVGGTWALELFQ"
     gene            1696..2016
                     /locus_tag="ID725_RS25830"
                     /old_locus_tag="pgaptmp_005166"
     CDS             1696..2016
                     /locus_tag="ID725_RS25830"
                     /old_locus_tag="pgaptmp_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HGV7853543.1"
                     /translation="MNDTEKPKVVFLSYNGLNRPVMVRGVPLMLFLSVGFLAVFGGFG
                     CMFMWGISGLIFPALCFLFLFIVRIICENDPNALSVMNLQLKGYFLKVMHRDSIIGFS
                     SVER"
     gene            2019..>2253
                     /locus_tag="ID725_RS25835"
                     /old_locus_tag="pgaptmp_005167"
     CDS             2019..>2253
                     /locus_tag="ID725_RS25835"
                     /old_locus_tag="pgaptmp_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGV7853544.1"
                     /translation="MQVAQLKDSKNKNVASMYPEYMYHISDSVVYTLDRKMLATIAIQ
                     GIPFEFASDSFLENSFNGIKDYLVGVGKEGDVYL"
BASE COUNT          642 a          381 c          495 g          735 t
ORIGIN      
        1 gtcggtcatg ctaagttcct gtaccttcga agaaataaaa ttatccatta tggtgcttaa
       61 cttttcccat agcccatcaa cgctggttgc tacatctcca gtacctgtta atgcattcga
      121 tagatcagac cctgcgtgca ttacaaatgg tattacatac tggctgtagt atggtgcagt
      181 atatgtgaat gcccctacta aacttaatga tgcgattagt tttgttactt cactaagaat
      241 aatgtctctt tgtgaatatg taatctcata agcaacatac acaatgtata aaagtacagc
      301 agcaacaaaa acgggagata ttgctttggc aattttagct gcattactgg ctgcaccact
      361 ttgaacaact gtattgatag tggttataaa ctctgacaca acgttcatac tacaactcct
      421 tttttggtga ttgctttttg atttttcatg gttgttacct ttggttagca aggtttctgg
      481 cttgatttta aattgattat aaaagctaaa acaggttgca taattgcaac ctacctaaaa
      541 agttttgtgt gaagtatatt ttgcgaatct taacttgatt cggtattgat tcatggcacg
      601 tatctcaacg tacttaacag taagactgac accagagtta aaccacttta tagaaatgga
      661 agctcgtaag catttaagaa gcaaacgaca gcaggcactg tgtatgcttt tatccaatat
      721 ggaggaatca aaaaaagaag atgattcttt caaccgtcgc tctgatgatg ttatttagcc
      781 aatgtgctcc aacggtatcg ccagtaacgc taaatgctat tgtgcaaaca gagtcaagcg
      841 gaaatcctta tatagttgca aatatatctg atggcatatc taaatccttt agcaataaag
      901 gatcggcggt agcgtacttg aatgctcttg caaagagtgg gaaacatttc agcgcaggac
      961 tgatgcaaat ctatagcaag aattttaaag cacttggtct tgataatgaa aatgttttcg
     1021 acccttgtaa aaatattaag gcaggggcag aaattctgac aaaaaactat cttgctaaaa
     1081 aaggaggaaa tgaacaagag aaccttttga agtctttgtc cgagtattat tcggggaata
     1141 gcacaagagg tttcaagaaa gaagctcaat ttaataatac gagttatgtc gaacgagtta
     1201 tgaagaacgc ttatgttgtt cctgagttgc gagtagataa acctgaaaat gcgaaagcaa
     1261 caaaagaaca gagtgttcag gaaaaaggta atcaaagttt tgtttcacac tcccaggact
     1321 gggatgtttt tggagattat tcgaatgaat aagtttgcta acgtaaatcg ttttgttttt
     1381 gcattatttt tcatgatgtt ttcagggttt gcggctgcgg caggtggcct tgattcagct
     1441 actaatgctc ttacgaacgt gaaaacctgg ctttttacct ttgttggtat tggtgctctt
     1501 gtttatatgc tttattgcat cctgatggcc tttatggaac gtaaaagctg gggggatgtt
     1561 ggtatggctc ttggctattg tgctcttgca ggcggtgcag tagtcggcgg tacgtgggcg
     1621 ctggaattgt tccagtaata acgaacgcta aaaagcgcgg gctgtttgtc agcctgcgca
     1681 attcaggagc taatgatgaa tgatacagag aaaccaaagg tcgtctttct ttcctataac
     1741 ggcctgaatc ggcctgtgat ggttaggggt gttccattga tgcttttttt gtctgtggga
     1801 tttcttgccg tgtttggtgg ttttggatgc atgtttatgt ggggaatttc tggactcata
     1861 tttcctgcat tatgcttctt gtttctgttc attgttcgca tcatttgtga aaatgaccca
     1921 aacgctctta gtgttatgaa tctccagttg aaggggtatt ttcttaaagt gatgcacaga
     1981 gactcaatta ttggtttttc ctctgtggag cgttgattat gcaggttgca caattaaaag
     2041 atagcaagaa taaaaatgtg gcatccatgt atcctgagta catgtaccat atttctgata
     2101 gtgttgttta tacacttgac agaaaaatgt tggctacaat tgctattcag gggattccct
     2161 ttgaatttgc atcagattct tttcttgaaa actctttcaa tggtatcaag gactatcttg
     2221 tcggggttgg caaagaaggt gatgtgtatt tgt
//
LOCUS       DABSYT020000269         2239 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.271, whole genome shotgun sequence.
ACCESSION   DABSYT020000269 DABSYT020000000
VERSION     DABSYT020000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.271"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            37..528
                     /gene="ygeG"
                     /locus_tag="ID725_RS25840"
                     /old_locus_tag="pgaptmp_005168"
     CDS             37..528
                     /gene="ygeG"
                     /locus_tag="ID725_RS25840"
                     /old_locus_tag="pgaptmp_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGV7853545.1"
                     /translation="MDTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNVTDAEIYYKLLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDASVKSKAQAYIELLTDNSE
                     DNG"
     gene            521..730
                     /locus_tag="ID725_RS25845"
                     /old_locus_tag="pgaptmp_005169"
     CDS             521..730
                     /locus_tag="ID725_RS25845"
                     /old_locus_tag="pgaptmp_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853546.1"
                     /translation="MAKHYNHLVKNAGYSVVTTMTSTSYAFLNQHLKTVKNTLKNIIF
                     QRVREQSILSKLNKAISDYISEFLR"
     gene            862..2238
                     /gene="ygeH"
                     /locus_tag="ID725_RS25850"
                     /old_locus_tag="pgaptmp_005170"
     CDS             862..2238
                     /gene="ygeH"
                     /locus_tag="ID725_RS25850"
                     /old_locus_tag="pgaptmp_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HGV7853547.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLKTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLRRFNPDYFVTGRINQNNAVNTLYIELTDAKNLFLIASNHLPVDE
                     LHNTSQFIIENILQTVHNPQRSVRLTKQDQGYKNHYLSDEMLAGKKELYEFTPESIYR
                     AMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKKNMKLYYKETESGSHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          827 a          374 c          378 g          660 t
ORIGIN      
        1 aatttagcat cacgagacac atcacagagg aatattatgg acacagaaac aattgaaata
       61 ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg cattatcgaa aggagaaaat
      121 ctggcattac tacatggttt gactcctgat atccttgata gaatatatgc atatgcattc
      181 gactaccatg aaaagggtaa tgtaacggac gcagaaattt attataaatt attgtgcatt
      241 tatgcgttcg aaaatcatga atatctaaaa ggttttgcat cagtatgtca gtcaaaaaag
      301 aaatatcaac aagcatatga cctttacaag ctaagttaca attactcccc gtatgatgac
      361 tattcagtta tttatcgtat gggtcaatgt caaattgggg ctaaaaatat cgataacgca
      421 atgcaatgtt tctatcatat tattaacaat tgtgaggatg ctagtgttaa gagtaaagcg
      481 caggcatata ttgaactctt aaccgataat tcagaagata atggctaagc attacaatca
      541 tttggttaaa aatgctggtt atagtgtagt aaccactatg accagcacct cttatgcttt
      601 cttaaatcaa catcttaaaa cagttaagaa cacactaaaa aatattattt ttcaacgcgt
      661 tagagaacaa tctattttat caaaattgaa taaggctatt tcagattaca tcagtgaatt
      721 cctaagataa attagtgaac acaaccatag atatgtcgaa tgtaaactcc tgtagcgtta
      781 atgacacata aatatgaata gccataattt ctattgctta tgcaggatgc aagaaaccaa
      841 ttttttcata gaggttaact aatggactta gaaaataaat tctcatatca ttttcttgag
      901 ggattaacgc tcacggaaga tggaattctt actcaaggaa atgagcaagt ttatattcca
      961 ccgaaagagt taggtgtatt aatagtatta cttgaatccg ctggtcatgt cgtactgaaa
     1021 gatatgatca tcgaatcagt atggaaaaat attattgtta gtgatgagtc cctgacaaga
     1081 tgtatctatt ctttgcgctg catttttgaa aaaattggct atgatcgttg catagaaaca
     1141 atctaccgga aaggttatcg tttcagtggg caggttttca aaactaaaat aaatgaagat
     1201 aacacttcag actattctat cgctatattc cctttcacta cttcattgaa aacactggat
     1261 ccattaatac ttaatcagga attagtacaa atcatttcaa ataaaaaaat cgatggcctc
     1321 tatacctatc cgatggctgc gacaaatttt tgtaatgatc acatatcgca aaattcattt
     1381 ttgagaagat tcaatccaga ttatttcgtt acaggaagaa taaaccagaa taatgcagtg
     1441 aacactttat acattgagtt gaccgacgct aaaaaccttt tcctcatcgc cagtaatcat
     1501 ctacctgttg atgaactaca taatacatca caatttatta tagaaaatat ccttcaaaca
     1561 gtacataatc cacaacgatc tgtaagatta actaagcagg accaaggata taagaatcat
     1621 tatttatcag atgaaatgtt agccggaaag aaagaacttt acgagttcac ccctgaaagc
     1681 atttacaggg ccatgactat atttgatgga ttacaaaata aaagtgatat acagacgcta
     1741 aaaacagaat gttattgcct tctagcggaa tgccatatgt ctttggcact tcatggaaaa
     1801 agtgaacttg aacttgctgc tcaaaaagca ttagagcttt tagattatgt atcagacata
     1861 accactgtcg atggaaaaat tttagctatt atgggactga taactggtct gtctggacaa
     1921 gcaaaagtat ctcatatctt atttgaacag gctaagatac actcaactga tatagcctct
     1981 ctctactact atagggcact tgtcaacttt cataatgaaa aaattgaaga ggcaaggatt
     2041 tgtatagaca aatcactaca actcgaaccc agaagacgaa aagcagttgt gataaaagaa
     2101 tgtgtagata tgtatgtgcc taacccgctc aaaaaaaaca tgaaactcta ctataaagaa
     2161 actgagagtg gaagccatcg agttataatt gacaacattt tgaaattaaa gcagctgacg
     2221 agaatttgta tgcgataaa
//
LOCUS       DABSYT020000270         2179 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.272, whole genome shotgun sequence.
ACCESSION   DABSYT020000270 DABSYT020000000
VERSION     DABSYT020000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.272"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..747)
                     /locus_tag="ID725_RS25855"
                     /old_locus_tag="pgaptmp_005171"
     CDS             complement(<1..747)
                     /locus_tag="ID725_RS25855"
                     /old_locus_tag="pgaptmp_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HGV7853548.1"
                     /translation="MIIRYGGGNHGIAEYLENGRKAEREYTRDELDHRFIIDGDLATT
                     NKIIESIEDKGQERYLHITLSFAENEISEETLKNVTSDFKKMFMNAYHTDEYSFYAEA
                     HLPKIKNIVDNKTGELVERKPHIHIVIPRTNLVTGRSLNPRGDLTNLKTQTQLDSVQE
                     YLNNKYNLISPKDSVRVSDENYANVLSRVKGDLYRERNNEVKKEVFSRVTNENINSTD
                     AFKNLLMQYGDVKVRNEGKTNQYFAVRLKDD"
     gene            complement(744..1082)
                     /locus_tag="ID725_RS25860"
                     /old_locus_tag="pgaptmp_005172"
     CDS             complement(744..1082)
                     /locus_tag="ID725_RS25860"
                     /old_locus_tag="pgaptmp_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein"
                     /protein_id="HGV7853549.1"
                     /translation="MKKTNKSVHVTFRLTEDEYAPFAKAIAQLDISKSEFFRMLTLNR
                     IENYQPDLRQQPDYKRCLFLMNKTSNNLNQIAHRLNLDHNKGIISSSLYERALNTLIN
                     IRDLLQGALK"
     gene            1428..1835
                     /locus_tag="ID725_RS25865"
                     /old_locus_tag="pgaptmp_005173"
     CDS             1428..1835
                     /locus_tag="ID725_RS25865"
                     /old_locus_tag="pgaptmp_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853550.1"
                     /translation="MRSIDAFLSPLCEAIRNEKLKSKQAAFNLYLQEILEAKKTYTWE
                     QICTYINQNTDSTLAVRAYRNMVERAKKKRLSQEKKNNESGVANKLPPQGDKSTQTTK
                     EPAIKGFFSQREKERKLEYDASATMAKFEDKYK"
     gene            1845..>2179
                     /locus_tag="ID725_RS25870"
                     /old_locus_tag="pgaptmp_005174"
     CDS             1845..>2179
                     /locus_tag="ID725_RS25870"
                     /old_locus_tag="pgaptmp_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HGV7853551.1"
                     /translation="MNTSINFILQGKGGVGKSFATSILSQYFIDEKKLENVVVADTDP
                     VNTTTAKVKRLNAEIIKIVENNNIVQSKFDSMFESILEGGNINFVIDNGASTFLPLLQ
                     YFDDNCVMD"
BASE COUNT          722 a          412 c          383 g          662 t
ORIGIN      
        1 gtcatcttta agacgcacag caaagtattg attagttttc ccctcattgc gaacctttac
       61 atcaccatat tgcattaata aatttttgaa tgcatctgtc gagttaatat tttcatttgt
      121 aactctggag aagacttcct tttttacttc attgttccgt tcacggtata aatcgccttt
      181 tactcgagac agcacatttg catagttttc atcacttaca cgaacggaat cctttgggct
      241 tatcagatta tatttgttgt taagatattc ctgaacagaa tcaagttggg tctgagtttt
      301 aagatttgtc agatcgcctc gtggatttaa tgacctacct gtaacaagat ttgttcttgg
      361 aatgacaatg tgaatatgtg gtttacgctc cacaagttca cctgttttgt tatcaacaat
      421 atttttaatt tttggcagat gggcttctgc ataaaatgaa tactcatccg tatgataggc
      481 attcatgaac atcttcttga aatcagaagt cacgttttta agagtttctt ctgaaatttc
      541 attttctgca aatgacaatg tgatatgcag gtatcgctcc tgccctttat cttctattga
      601 ttcaattatt ttatttgttg tagccagatc gccgtcaatg ataaatctat ggtcaagctc
      661 atcacgggta tactcacgct ccgcttttct tccattttca aggtattctg caataccatg
      721 attgccaccg ccatagcgta ttatcatttt agtgcgccct gcaataaatc acggatattt
      781 atcaaggtat taagcgccct ttcatacaat gaagatgata taattccctt gttatgatcc
      841 agatttaaac gatgtgcaat ctgattaaga ttattgcttg ttttgttcat aagaaaaaga
      901 caacgcttgt aatcaggttg ttgtcgaaga tctggctgat agttctctat cctattgagc
      961 gtcaacattc taaaaaactc agatttacta atatcaagct gtgctattgc tttagcaaac
     1021 ggcgcatatt catcctcagt taacctaaat gtgacatgta cgctcttgtt tgtttttttc
     1081 attttagccc tcaaggttcg tagaaccgca taagaacagc tcaagtcgtt cggggtttca
     1141 aagggaacac cctttggcac gtcgattgta acatacccgg aacaggtttg tgtacaatcg
     1201 gtctcgtgcc tgtgttttag aaaacgattc agactagaca cacaaaacga cacacacaat
     1261 agcaccacag acacatataa tgacacacaa aagaacacac ataaaaacac acaaaaaacc
     1321 ttgacaacac acaaaacgac acacacaatg tcacacataa ggacacacac attgcaattt
     1381 agggatagtt ttgtcatatt gggaggtaaa tatatagagg gtaacatatg cgaagcatcg
     1441 acgcgttctt atcaccactt tgtgaagcga taagaaatga aaaactaaaa tcaaaacaag
     1501 cagcattcaa tctgtacctt caagagatac ttgaagccaa gaaaacatat acctgggaac
     1561 agatatgcac atatataaat cagaatactg attcgacgct ggctgtacgt gcttatagaa
     1621 atatggttga acgagcaaaa aagaaacgct tgtcacagga aaagaaaaat aacgaatctg
     1681 gagttgcaaa caagttgccg cctcaaggtg acaaatcgac tcaaacaaca aaagaaccag
     1741 cgataaaagg ttttttctct caaagagaaa aagagagaaa actggaatat gatgcttcgg
     1801 caacaatggc aaaatttgaa gacaaataca aataaggatg aaacatgaat acttcaatca
     1861 attttatatt gcaaggtaaa ggcggtgtag gtaaatcttt tgccacatca atactgtctc
     1921 aatattttat tgatgaaaaa aaacttgaaa atgttgttgt cgcggataca gacccggtaa
     1981 acacaactac agcaaaagta aaacgcctta atgctgaaat tatcaagatt gtagaaaata
     2041 acaacattgt gcaatcaaaa tttgatagca tgtttgaatc tattcttgaa ggcggaaaca
     2101 ttaatttcgt tattgataat ggtgcttcta cgttcttgcc attacttcag tatttcgatg
     2161 ataactgtgt aatggatat
//
LOCUS       DABSYT020000271         2162 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.273, whole genome shotgun sequence.
ACCESSION   DABSYT020000271 DABSYT020000000
VERSION     DABSYT020000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.273"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            11..526
                     /gene="ehxC"
                     /locus_tag="ID725_RS25875"
                     /old_locus_tag="pgaptmp_005175"
                     /gene_synonym="hlyC"
     CDS             11..526
                     /gene="ehxC"
                     /locus_tag="ID725_RS25875"
                     /old_locus_tag="pgaptmp_005175"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HGV7853552.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            528..>2162
                     /gene="ehxA"
                     /locus_tag="ID725_RS25880"
                     /old_locus_tag="pgaptmp_005176"
     CDS             528..>2162
                     /gene="ehxA"
                     /locus_tag="ID725_RS25880"
                     /old_locus_tag="pgaptmp_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HGV7853553.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQRAGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRER"
BASE COUNT          700 a          371 c          497 g          594 t
ORIGIN      
        1 tttaaacatt atgaaatcta atgcttttga cgtcatgggg aaggttgcat ggctatgggc
       61 ctgttctcct ctgcataaaa aatggccatt atctgttttt gctattaatg tcatacctgc
      121 tattcagaca aatcaatttg ctctcttaat aaaggatgag cttcctgtag ctttttgtag
      181 ctgggccagt ttagatctgg aatgtgaggt aaaatatata aatgatgtaa catctttgta
      241 tgcaaaagac tggatgtcgg gagaaagaaa gtggtttatt gactggatag ctccatttgg
      301 acacaacatg gagttatata aatacatgag gaaaaagtat ccttatgagt tattcagagc
      361 aataaggtta gatgagagca gtaaaacagg aaaaatagct gaatttcatg gaggggggat
      421 agataaaaaa ctggcatcaa aaatattcag gcaataccat catgaactta tgtctgaagt
      481 aaaaaacaga caagatttta attttaatat tgagaaagaa aactaatatg acagtaaata
      541 aaataaagaa cattttcaat aatgcgacat tgactacaaa atcagcattt aatacagcat
      601 catcaagcgt acgttccgct ggaaaaaaac tcatattatt aatacctgat aattatgaag
      661 ctcagggcgt gggtattaat gagttggtca aagctgctga tgagcttgga atagaaatac
      721 accgtactga acgagatgat acagcgattg caaaccagtt ttttggtgca gcagaaaaag
      781 ttgtaggatt aactgaacgt ggtgttgcaa tattcgcacc acaacttgac aaacttctgc
      841 agaagtatca gaaagttggg agtaaaatag gaggaaccgc tgaaaatgta ggtaataatc
      901 tgggaaaagc cggaacagtt ctctcagcac tacagaattt tacggggatt gctttatcag
      961 gcatggctct tgatgaattg ctgagaaaac aacgggcagg agaggatata agtcagaatg
     1021 atattgccaa aagtagtatt gaacttatta atcagcttgt agatacagta tcaagtataa
     1081 acagtaccgt tgattcattt tctgagcagc ttaaccagct tggctcattt ttatccagta
     1141 aacctcgatt aagttctgtt ggtgggaaat tacaaaattt accagacctg ggccccctgg
     1201 gggatgggct ggatgttgtc tccggaattc tttctgctgt atcagcaagc tttattctgg
     1261 gaaacagtga cgcacataca ggaacaaaag ctgcagcggg tatcgaactg acaactcagg
     1321 ttcttggaaa tgttggtaaa gctgtttcgc aatatattct ggctcagaga atggcacagg
     1381 ggttatcgac aacagctgca agtgcgggtc tgatcacatc ggctgttatg ctggctatca
     1441 gtcctctttc tttcctggct gctgcagata aatttgagcg agctaagcag cttgaatcat
     1501 attctgaacg atttaaaaaa ttgaattatg aaggggatgc tttactcgca gcctttcata
     1561 aagaaaccgg agctatagat gcagccctga caacaataaa tactgtcctg agttctgtat
     1621 ctgcgggagt tagtgcagcc tccagtgcat ccctcatagg ggccccgata agcatgctgg
     1681 tgagtgcatt aaccggtacg atatctggca ttctggaagc atcaaaacag gctatgtttg
     1741 agcacgttgc agagaaattc gctgctcgga tcaatgaatg ggaaaaggag catggcaaaa
     1801 attattttga gaatggatat gacgcaagac atgctgcgtt tttagaagac tctctgtctt
     1861 tgcttgctga tttttctcgt cagcatgcag tagaaagagc agtcgcaata acccagcaac
     1921 attgggatga gaagatcggt gaacttgcag gcataacccg taatgctgat cgcagtcaga
     1981 gtggtaaggc atatattaat tatctggaaa atggagggct tttagaggct caaccgaagg
     2041 agtttacaca acaagtgttt gatcctcaaa aagggaccat agacctttca acaggtaatg
     2101 tatcaagtgt tttgacattt ataacaccaa catttacccc aggagaagaa gttagagaaa
     2161 ga
//
LOCUS       DABSYT020000272         2114 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.274, whole genome shotgun sequence.
ACCESSION   DABSYT020000272 DABSYT020000000
VERSION     DABSYT020000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.274"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1330)
                     /locus_tag="ID725_RS25885"
                     /old_locus_tag="pgaptmp_005177"
     CDS             complement(<1..1330)
                     /locus_tag="ID725_RS25885"
                     /old_locus_tag="pgaptmp_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGV7853554.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDA"
     gene            complement(1276..1497)
                     /locus_tag="ID725_RS25890"
                     /old_locus_tag="pgaptmp_005178"
     CDS             complement(1276..1497)
                     /locus_tag="ID725_RS25890"
                     /old_locus_tag="pgaptmp_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853555.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1542..>2114)
                     /locus_tag="ID725_RS25895"
                     /old_locus_tag="pgaptmp_005179"
     CDS             complement(1542..>2114)
                     /locus_tag="ID725_RS25895"
                     /old_locus_tag="pgaptmp_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGV7853556.1"
                     /translation="RLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFG
                     QFVAANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVG
                     DQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEPGGDSTTPSPVELVSMFQTGSVAI
                     RAERWINWRRRRTAAVAVITGVNYGSASGG"
BASE COUNT          412 a          689 c          546 g          467 t
ORIGIN      
        1 ccgcatcatg ctctgtttca ctgattgcct tattaccgtc agatgcgccg tcaggcagct
       61 gtgatgaaac tgttttcccg gcagacgcga acggatcctc acgggcatca cgacgggaca
      121 gcgcctccag actgtagttc tgctgctgaa gatacagtgc atcaccgccg gccaggggcg
      181 gcaggttctc ccgtttacgg gcctcattgg gcgtgagaag cgtatttttc accgcatccc
      241 ccagcgtttt catgcgccgc tcactgtcca ttctcagcag cgtggtgaca tcaaattctg
      301 tactctcgtt ttcccccgtt tccagcgcct catccagtaa cagttcaatg gactcaatca
      361 gcgtctgcag gcactgggaa taatactgct gctccagcgc ctccacgttg tcactggaag
      421 gcggttgtcc cacgccaatc ttgtaggccg ggacacggaa caccgaacag acaatttcag
      481 cggtcatctt cagttgttcc accgtctgcg catccaccgg tgaaaacgtc gtggggttgt
      541 attttgcccc gttgctcaga atggccgttt tccccgcatt ttcgcctgta tacccgctgt
      601 cccagttgct cttcagtttt ttcgcatttt cttccgtaat actgccgggg atctcaatca
      661 ccccggacgg cctgccgcca tttctgaaaa aagacgtcga attttcctga atatgatgcc
      721 cctgcgtggc cgccagcccg gcggcataca ccggcggcaa tcctataagc ggatgaaaaa
      781 aacagttaaa ccggtcgtgg atcacttccc gggcaggcac cgtcaccgcc tccgtgatcc
      841 cgcagttccg gtccggcgtg atgcggtaga acacctcgcc gtcatccgcc accagaggtt
      901 caacccggct ccagtccaga atacgcagtt ctttgatctg cccccgggca ttacggattt
      961 tcagcaccac cgtattgcca tgacgcagtt tggcgttcag ccacagttca aaaaactgga
     1021 tgcggttctg ctgggcgttg ggacgacgac agaggcgggc aatatccccc cggcgcgttt
     1081 ccctgcgtat cccatgcgca tccgtctgca taagacgcag ccgcattttg gcgatatcct
     1141 gggatatcag cgaaatacat gcaaacaccg catgaaagga gaggacggct tcaggatcgg
     1201 ctttcacgcc ctgctgccag gcgccggaaa agggctcagc caccgcctga aacaggctgg
     1261 tccagcccgc ctctcttacg tcacgtcctg atttctggtt ttttcgggtt cgccgtaaaa
     1321 ggttccacat tcgccatgct ccgcatcacg tttctttttc tgacctgccg gacgtcgcac
     1381 cgtgatgtac tccgcctttc ccaggcgaac cagcacctcc gcacacggct gtgccacatc
     1441 acggatatcc ccggcccggg catcatgcgt gccctgcaga tatcggatct ttgccataac
     1501 ctgttacggg aggcgcacgc ctcccgtcct ccttatcaga ctcagccgcc ggacgcactg
     1561 ccgtagttca ctccggtgat caccgccacc gccgcagtac ggcgacgacg ccagttgatc
     1621 cagcgctccg cacggatggc cacgctgcct gtctggaaca tggaaaccag ctccaccggg
     1681 gacggcgtgg tactgtcgcc gcccggctca gactgcattt ccagtgatgc ctcgcgggac
     1741 atatccactg ccacgccgcc gtcatccgcc agataaatat ccggggcatt caccagcacc
     1801 agctggtcac ccacgtactg ggagacaatc accggcagcc cctggaagga gccacccagc
     1861 agggtcatgt ccgggtattc cttctgaccc agcgcatttt tacgcatgga cagtgccagg
     1921 gcattggtgc tggacatcag ccagaccgca ccggtgggct gcaggtttgc tgccacaaac
     1981 tgtccaaacg cagcctcggc atccgcatcc gggttaccgg ttgatgccgt gcccttcaca
     2041 tcatgggtga tggacgccgg ggagacatct gccactgcgg cttttttcgg gtccacaaag
     2101 tctgtatcca gacg
//
LOCUS       DABSYT020000273         2076 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.275, whole genome shotgun sequence.
ACCESSION   DABSYT020000273 DABSYT020000000
VERSION     DABSYT020000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.275"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..183
                     /locus_tag="ID725_RS25900"
                     /old_locus_tag="pgaptmp_005180"
     CDS             <1..183
                     /locus_tag="ID725_RS25900"
                     /old_locus_tag="pgaptmp_005180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853557.1"
                     /translation="WRLAVVRCGWQQTITVTTRSPTKRSAIKKPESWNRRMPPLLTCR
                     CASVMLLRSMQNTRGN"
     gene            187..393
                     /locus_tag="ID725_RS25905"
                     /old_locus_tag="pgaptmp_005181"
     CDS             187..393
                     /locus_tag="ID725_RS25905"
                     /old_locus_tag="pgaptmp_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGV7853558.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            458..682
                     /locus_tag="ID725_RS25910"
                     /old_locus_tag="pgaptmp_005182"
     CDS             458..682
                     /locus_tag="ID725_RS25910"
                     /old_locus_tag="pgaptmp_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853559.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1039..1179
                     /locus_tag="ID725_RS25915"
                     /old_locus_tag="pgaptmp_005183"
     CDS             1039..1179
                     /locus_tag="ID725_RS25915"
                     /old_locus_tag="pgaptmp_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853560.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1309..1494)
                     /locus_tag="ID725_RS25920"
                     /old_locus_tag="pgaptmp_005184"
     CDS             complement(1309..1494)
                     /locus_tag="ID725_RS25920"
                     /old_locus_tag="pgaptmp_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGV7853561.1"
                     /translation="MSSKNRPRRTTIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            1536..1901
                     /locus_tag="ID725_RS25925"
                     /old_locus_tag="pgaptmp_005185"
     CDS             1536..1901
                     /locus_tag="ID725_RS25925"
                     /old_locus_tag="pgaptmp_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGV7853562.1"
                     /translation="MAVLRTLPGRIKTLNTRRINVLRGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
BASE COUNT          488 a          466 c          549 g          573 t
ORIGIN      
        1 tggcggttgg ctgtggtgcg ctgtggctgg caacaaacca ttaccgtgac aacgcgctca
       61 cctacaaagc gcagcgcgat aaaaaagcca gagagctgga acaggcgaat gccaccatta
      121 ctgacatgca ggtgcgccag cgtgatgttg ctgcgctcga tgcaaaatac tcgagggaat
      181 tagccgatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgct ggtcgtaagc
      241 gcctgcggat caacgccacc tgccccggta ccgtgcgtga agccaccggc acctccggcg
      301 tgggcaatga tgccgccgtc gaactctctc cggttgctgg acgaaacgtt ctcggtatca
      361 gagacggaat catcagcgac caggcagcat tgagaatgct tcaggaatat atccgcactc
      421 agtgtattaa ctagtatttt tgttattcgg agaatgcatg aagaaattat tggtaaccgt
      481 aaagcctttt cagggaacaa ttccgttccg tattttgcag cgtggtcgtg ttcttgttga
      541 aggttcgttc agtggtaaat gtacgcaatt acactccagg acctttcagg tgaatgccac
      601 gaatgaagag ctaaccgttg agtgtacgat gaatgccgct aaatgccgca tggtatccgc
      661 tgcattacag ccagtgtgtt gagcgacctt attattcatg cgcggtattg tcgccgtatt
      721 cctgcattaa cagagaccgc agcccgaccg ggagaatcct ctgcgcgagt gtgcggggat
      781 aatcaaaaac gatacacacc ggggtttacc gcgttaacgg agcgcggcgt tgtcccctca
      841 tggtcgctgg tccggtgcga tggtggaaga agccggatgt ttatcactat taattgataa
      901 cacagaaatg gattcattga ttttcagcac gtttttgtat tcgtattatt gaacatctgt
      961 ttattttact tttaacatat tgataataaa aagagctgta aatctttaga tgagtcgatt
     1021 ttgtccgggg aagttcaaat ggattttatg ctgacggttt ctggtgtggt tatcctgtcc
     1081 attgcttata ctgcagataa atatggctgc catttgttat cacgtattgg cgcttattgc
     1141 tcgttgatgc tgattttctc gtcgcttttt tttgagtaag ttatattaat tataacaaat
     1201 aattttctgt gttttttcag gctatcccgt cagacgggaa gcctgtactg ccgggggacg
     1261 aatggaaaac tgatgtgtcc ggtaactgtg tgttctgtga acaccatgtt acttaattat
     1321 gtaattcata cccgaacgct ctgttgacag ccttcttctg caggcttcaa taacccacgc
     1381 tgaaaagtta ccggaacctt tatgttcaag ggcgatattg atctgttcaa tcatgtgatt
     1441 ggggaaacgg atattgcgga ttgtggttct gcggggccgg tttttcgatg acattttctt
     1501 tcctctggtg acaagctata tggcgaggat tttacatggc tgtgcttcgt acgttaccgg
     1561 gcagaatcaa aactctgaac acccggcgga taaatgtcct gaggggggaa cagcgtcgtg
     1621 tcagtggcag tgcccgggtt tccctcaaac gtcgtatctg gcggagggat gccggacact
     1681 gttgtctctg tagacgcgtg gttgaccttt gtgacagtga actcgatcac cgcattgcac
     1741 ttcagttcgg tggtggtaat gaggagacga acctctggac cctctgtact gaatgccatc
     1801 gccagaagtc agcgagtgaa gcggcgagtg gtatgcctga tccgacgttg cctgagcttc
     1861 ctgatggcac gctcagggcc gacggaatca ctggcctgtg accagacccg ggggggatca
     1921 tccggcgaaa aaaaacgatc gccctggaca ccgcgccccc tctcacgcag agaaaaaatt
     1981 cccgtttcag ggcagttaac atgttaactg gctgcccggg catttttgcg gtttttatct
     2041 ttattattca gtttgttgtg cggaaaaaat gttaac
//
LOCUS       DABSYT020000274         2072 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.276, whole genome shotgun sequence.
ACCESSION   DABSYT020000274 DABSYT020000000
VERSION     DABSYT020000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.276"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            1..402
                     /gene="eptO"
                     /locus_tag="ID725_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /gene_synonym="gspS"
     CDS             1..402
                     /gene="eptO"
                     /locus_tag="ID725_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HGV7853563.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            581..1752
                     /locus_tag="ID725_RS25935"
                     /old_locus_tag="pgaptmp_005187"
     CDS             join(581..830,830..1752)
                     /locus_tag="ID725_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGV7853564.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
BASE COUNT          598 a          456 c          533 g          485 t
ORIGIN      
        1 atgatgggta atattttgaa aaaactgaat tgtatcgcgt cattactagt cttagtgact
       61 atctctggtt gtcatcagtc accctccatc cataagcagg ccactgttcc tccgagcgaa
      121 caactggaac aaatggcgtc gattgtctct gcgacacggt atctgaaaat gagatgtaac
      181 cgtagtgatt taccagatga acagtcgatc ctaaatgtcg ctaaccggat cgctatcggg
      241 aaagggtggc agtcattaac tcaggaggat atacgtaaac acagcgatga tatctatgtg
      301 cgtctgacta gggacagtac gcctgagtat ataaaatgca gagaattcaa tcggcggctg
      361 gtcccattta tcggtgaatt attggcaagg ggtcgtggat aatatgaacc gccccggaaa
      421 tcctggagac taaactccct gagaaagagg taaacagcat gaataaaaat gggtcttccc
      481 ttgtagtggt ggctgaaggc atgatgattg tagtggcaca ctgaatttgg ccacctgaac
      541 agaggtgata tgctcacctc agaacaacac aggtgctcca atgaaaaaaa gaaatttcag
      601 cgcagagttt aaacgcgaat ccgctcaact ggttgttgac cagaactaca cggtggcaga
      661 tgccgccaaa gctatggata tcggcctttc cacaatgaca agatgggtca aacaactgcg
      721 tgatgagcgt cagggcaaaa caccaaaagc ctctccgata acaccagaac aaatcgaaat
      781 acgtgagctg aggaaaaagc tacaacgcat tgaaatggag aatgaaatat taaaaaaggc
      841 tacaaccgcg ctcttgatgt cagactccct gaacagttct cgataatcgg gaaactcaga
      901 gcgcattatc ctgtggtcac actctgccat gtgttcgggg ttcatcgcag cagctacaga
      961 tactggaaaa accgtcctga aaaaccagac ggcagacggg ctgtattacg cagtcaggta
     1021 cttgagttgc ataacatcag ccatggttct gccggggcaa gaagcatcgc cacaatggca
     1081 accctgagag gcttcagaat ggggcgctgg cttgccggca ggctcatgaa agaactggga
     1141 ctggtcagtt gccagcagcc tgcgcaccgt tataaacgag gtggtcgtga acatgtcact
     1201 atcccgaatc accttgggcg gcagttcgca gtgacagagc caaatcaggt atggtgcggc
     1261 gacgtgacgt acatctggac ggggaaacgt tgggcatacc ttgccgttgt tctcgacctg
     1321 tttgcaagga aaccggtagg ttgggcaatg tcgttctctc cggacagcag actgaccatc
     1381 aaagcgctga aaatggcctg ggaaatccgc agtaaaccag ccggggtaat gttccacagc
     1441 gatcagggca gccactatac aagcaggcag ttccggcagt tactgtggcg ttaccagatc
     1501 aaacagagtc tgagtcgacg aggaaattgc tgggataaca gcccgatgga gcgcttcttc
     1561 aggagtctga aaaacgagtg gataccggtg acgggttaca tgaacttcag cgatgctgcc
     1621 catgaaataa cggactatat cgttgggtat tacaacgcgc tcaggccgca cgaatataac
     1681 ggtgggttgc caccaaatga atcggaaaac cgatactgga aaaactctaa agcggtggcc
     1741 agtttttgtt gaccactaca tcccgtcagc cgatttacgg gccgtaagta acgaagttgg
     1801 atgcaaatgt cagatcgtgt gcgcctgtta tggctcggct ggtaagagag ccttataggc
     1861 gcatttgaaa aaactccagc tataccggaa gagatttatt attaacagga gaggtttgct
     1921 tttctgtgta ggtttagatt ttgtgtttgc agggtaaaca aaactgatta actcgtcatt
     1981 tcggtaactg aaatagtttg ctgtattctg tttacaatct ctttaataag gcatttgtca
     2041 cgtggctatt catatgaaaa tcatacgtta tc
//
LOCUS       DABSYT020000275         2065 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.277, whole genome shotgun sequence.
ACCESSION   DABSYT020000275 DABSYT020000000
VERSION     DABSYT020000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.277"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..76
                     /locus_tag="ID725_RS25940"
                     /old_locus_tag="pgaptmp_005188"
     CDS             <1..76
                     /locus_tag="ID725_RS25940"
                     /old_locus_tag="pgaptmp_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853565.1"
                     /translation="IVNYFIWYKCSFKKHIDRQLNIRG"
     gene            79..1173
                     /gene="rfbE"
                     /locus_tag="ID725_RS25945"
                     /old_locus_tag="pgaptmp_005189"
                     /gene_synonym="perA"
     CDS             79..1173
                     /gene="rfbE"
                     /locus_tag="ID725_RS25945"
                     /old_locus_tag="pgaptmp_005189"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HGV7853566.1"
                     /translation="MKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKFAE
                     QNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVDS
                     DNETWQMSVSDIEQKITNKTKAIMCVHLYGHPCDMEQIVELAKSRNLFVIEDCAEAFG
                     SKYKGKYVGTFGDISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHRQY
                     WHDVIGYNYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKDVF
                     HTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDLGW
                     RGINLPSFPSLSNEQVIYICESINEFYSDK"
     gene            1198..>2065
                     /locus_tag="ID725_RS25950"
                     /old_locus_tag="pgaptmp_005190"
     CDS             1198..>2065
                     /locus_tag="ID725_RS25950"
                     /old_locus_tag="pgaptmp_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HGV7853567.1"
                     /translation="MKIALNSDGFYEWGGGIDFIKYILSILETKPEICIDILLPRNDI
                     HSLIREKAFPFKSILKAILKRERPRWISLNRFNEQYYRDAFTQNNIETNLTFIKSKSS
                     AFYSYFDSSDCDVILPCMRVPSGNLNKKAWIGYIYDFQHCYYPSFFSKREIDQRNVFF
                     KLMLNCANNIIVNAHSVITDANKYVGNYSAKLHSLPFSPCPQLKWFADYSGNIAKYNI
                     DKDYFIICNQFWKHKDHATAFRAFKIYTEYNPDVYLVCTGATQDYRFPGYFNELMVLA
                     KKLGIESKIKILG"
BASE COUNT          729 a          294 c          375 g          667 t
ORIGIN      
        1 tattgtaaat tactttatat ggtataaatg tagttttaaa aaacatatcg atagacagtt
       61 aaatataaga ggatgaaaat gaaatatata ccagtttacc aaccgtcatt gacaggaaaa
      121 gaaaaagaat atgtaaatga atgtctggac tcaacgtgga tttcatcaaa aggaaactat
      181 attcagaagt ttgaaaataa atttgcggaa caaaaccatg tgcaatatgc aactactgta
      241 agtaatggaa cggttgctct tcatttagct ttgttagcgt taggtatatc ggaaggagat
      301 gaagttattg ttccaacact gacatatata gcatcagtta atgctataaa atacacagga
      361 gccaccccca ttttcgttga ttcagataat gaaacttggc aaatgtctgt tagtgacata
      421 gaacaaaaaa tcactaataa aactaaagct attatgtgtg tccatttata cggacatcca
      481 tgtgatatgg aacaaattgt agaactggcc aaaagtagaa atttgtttgt aattgaagat
      541 tgcgctgaag cctttggttc taaatataaa ggtaaatatg tgggaacatt tggagatatt
      601 tctactttta gcttttttgg aaataaaact attactacag gtgaaggtgg aatggttgtc
      661 acgaatgaca aaacacttta tgaccgttgt ttacatttta aaggccaagg attagctgta
      721 cataggcaat attggcatga cgttataggc tacaattata ggatgacaaa tatctgcgct
      781 gctataggat tagcccagtt agaacaagct gatgatttta tatcacgaaa acgtgaaatt
      841 gctgatattt ataaaaaaaa tatcaacagt cttgtacaag tccacaagga aagtaaagat
      901 gtttttcaca cttattggat ggtctcaatt ctaactagga ccgcagagga aagagaggaa
      961 ttaaggaatc accttgcaga taaactcatc gaaacaaggc cagtttttta ccctgtccac
     1021 acgatgccaa tgtactcgga aaaatatcaa aagcacccta tagctgagga tcttggttgg
     1081 cgtggaatta atttacctag tttccccagc ctatcgaatg agcaagttat ttatatttgt
     1141 gaatctatta acgaatttta tagtgataaa tagcctaaaa tattgtaaag gtcattcatg
     1201 aaaattgcgt tgaattcaga tggattttac gagtggggcg gtggaattga ttttattaaa
     1261 tatattctgt caatattaga aacgaaacca gaaatatgta tcgatattct tttaccgaga
     1321 aatgatatac attctcttat aagagaaaaa gcatttcctt ttaaaagtat attaaaagca
     1381 attttaaaga gggaaaggcc tcgatggatt tcattaaata gatttaatga gcaatactat
     1441 agagatgcct ttacacaaaa taatatagag acgaatctta cctttattaa aagtaagagc
     1501 tctgcctttt attcatattt tgatagtagc gattgtgatg ttattcttcc ttgcatgcgt
     1561 gttccttcgg gaaatttgaa taaaaaagca tggattggtt atatttatga ctttcaacac
     1621 tgttactatc cttcattttt tagtaagcga gaaatagatc aaaggaatgt gttttttaaa
     1681 ttgatgctca attgcgctaa caatattatt gttaatgcac attcagttat taccgatgca
     1741 aataaatatg ttgggaatta ttctgcaaaa ctacattctc ttccatttag tccatgccct
     1801 caattaaaat ggttcgctga ttactctggt aatattgcca aatataatat tgacaaggat
     1861 tattttataa tttgcaatca attttggaaa cataaagatc atgcaactgc ttttagggca
     1921 tttaaaattt atactgaata taatcctgat gtttatttag tatgcacggg agctactcaa
     1981 gattatcgat tccctggata ttttaatgaa ttgatggttt tggcaaaaaa gctcggaatt
     2041 gaatcgaaaa ttaagatatt agggc
//
LOCUS       DABSYT020000276         2046 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.278, whole genome shotgun sequence.
ACCESSION   DABSYT020000276 DABSYT020000000
VERSION     DABSYT020000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.278"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(554..1081)
                     /locus_tag="ID725_RS25955"
                     /old_locus_tag="pgaptmp_005191"
     CDS             complement(554..1081)
                     /locus_tag="ID725_RS25955"
                     /old_locus_tag="pgaptmp_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGV7853568.1"
                     /translation="MDNSKHSDEAEKLLAELSARKGEGEGEGEGEGEPKSTVSVFYLQ
                     PEEVNTLSHQAKRGDGEAGFRLFLYYKLSNYDEEKSSQWLKIAANNGHATAQYRLYVE
                     LQEQGETKEANEWLQKIKLAASNNDSSAIWFLVNCYKNQGNKKEALQWAYKLKASKQP
                     LDADRLIEELTKEQK"
     gene            complement(1150..>2046)
                     /locus_tag="ID725_RS25960"
                     /old_locus_tag="pgaptmp_005192"
     CDS             complement(1150..>2046)
                     /locus_tag="ID725_RS25960"
                     /old_locus_tag="pgaptmp_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGV7853569.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEELGL
                     YYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSPSDPI
                     YKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEGMPVV
                     LFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGKDNGV
                     FELVPDEKQPGDFHKFTPTGSK"
BASE COUNT          544 a          498 c          353 g          651 t
ORIGIN      
        1 gaaatataag gaattattat acaagctatt aaatcaacaa taaaagatct tttaaaaaat
       61 aacaaaaaac agaacactat caaaacataa aaagcaaaca aaaaacaata agcatgttga
      121 ttgaatattt taaatacatg ttcttttaca ataaaaaaat gaatggttat cgaacaagcc
      181 agcaataata accttattat tttcataagc tccatccact aaaatgctgg agatataaaa
      241 aatgaaccaa acggcttctt tggcccatac ttaggtttgg ggtgtagaat cttagtcata
      301 agatctatgc cgagtcatgt gctggccaca tacttaatga tacaaaaagc tatattttca
      361 tcttttctct tgcgactttt gatgcatctg ccaagtacat cgtaatgata accggcgacg
      421 aagatgccag ttttggtttc aacttgcgct ttgatgagct tgttgcagtc gtcgtaaacg
      481 taattttgtg tgtaggtgtt gtagcgactg gtaaagtttt tctactctcc atttttctgt
      541 tacctgttta aagctacttt tgttctttcg ttaattcttc aattagtcga tctgcatcaa
      601 gcggctgttt actcgctttg agtttatatg cccattgcaa tgcttctttt ttatttccct
      661 ggtttttata acaattcacc aagaaccata ttgctgaact gtcattatta ctggcagcca
      721 atttaatttt ttgtaaccat tcattagctt ccttggtttc accttgttct tgtaactcta
      781 catataaccg atactgagct gtcgcatgac cattattagc cgctattttt aaccactgac
      841 tacttttttc ttcatcataa ttggacaact tgtagtacaa gaacaaccta aaacccgcct
      901 cgccatcgcc tcgttttgcc tggtgtgata aagtatttac ttcttctggc tgcaaataaa
      961 agacgcttac tgttgatttt ggttcacctt caccttcacc ttcaccttca ccttcacctt
     1021 ttctagcaga aagctccgcc aataattttt ccgcctcatc agaatgtttt gaattatcca
     1081 tagcagccct cactccaaag ttataaagac ataccatgat aatcaacaaa aaagtaaata
     1141 ctttcattac tatttgcttc ctgtaggtgt aaacttatga aagtcccctg gttgtttctc
     1201 gtctggcacc aattcaaaca ccccgttgtc cttaccgcct attgttatcc tagctgtttg
     1261 ccccataata gcatagtttc catccggcca aatccatatt atttcatcag gagcaatcac
     1321 tgtagcatca agttcgttag ctagtttctg tgcaaatgaa tttttgcctt ttcctgtttc
     1381 gcaggaaaac aagactacag gcataccttc atgatattta ggattcgctc ttatgtcgct
     1441 agctaattgt tttattgtca gatcacttcc ttttttgatt ctgtcatcac tcatagatgt
     1501 aggcgaacca tgacctccaa ctgtaaaacc cgttggaaat tcccttacat taagagaacc
     1561 tttatatata ggatctgacg gagaataata attcaaatca agtgctaacc ccaacggatc
     1621 tatatgttca ataggattga gtgggtactg atacaggttc catcccccct ccagccctat
     1681 cgggtcctgg gtgatatatc tcccctgcaa cggatcatag taccggttgc ggttgtagta
     1741 cagccccaac tcctcatcat actgctgccc cggcagacgg agtgactgtt gaaggtgctg
     1801 cgcgctggtt tcgcccagca ggtttcccca ctcgtcatac tccccctgcc acgccgtttc
     1861 cccttccggg ctgatgagcg ccagcggcag tccccggtgg tcgcagtggt aaagatgaag
     1921 tttcctctcc gggatgtatt ccgcctccag ctgcgcggcc atctgctccg ccgtcagccc
     1981 gcactgcgca agccactgct ggctttcttc actcacgctg ccctgccgca gctcccgctc
     2041 cagcct
//
LOCUS       DABSYT020000277         2029 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.279, whole genome shotgun sequence.
ACCESSION   DABSYT020000277 DABSYT020000000
VERSION     DABSYT020000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.279"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..560)
                     /locus_tag="ID725_RS25965"
                     /old_locus_tag="pgaptmp_005193"
     CDS             complement(<1..560)
                     /locus_tag="ID725_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7853570.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSP"
     gene            658..885
                     /locus_tag="ID725_RS25970"
                     /old_locus_tag="pgaptmp_005194"
     CDS             658..885
                     /locus_tag="ID725_RS25970"
                     /old_locus_tag="pgaptmp_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGV7853571.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            882..1307
                     /locus_tag="ID725_RS25975"
                     /old_locus_tag="pgaptmp_005195"
     CDS             882..1307
                     /locus_tag="ID725_RS25975"
                     /old_locus_tag="pgaptmp_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGV7853572.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            1376..>2029
                     /locus_tag="ID725_RS25980"
                     /old_locus_tag="pgaptmp_005196"
     CDS             1376..>2029
                     /locus_tag="ID725_RS25980"
                     /old_locus_tag="pgaptmp_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HGV7853573.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     "
BASE COUNT          548 a          453 c          519 g          509 t
ORIGIN      
        1 gggcttaaat acacaagctt cccatcaata actaatttct ttatagtcat agatggttca
       61 tttttaagcg gatccggaac tattacagcg acgatgctgc catttttata actttctcct
      121 ggccttagga tcacagtagc accaactggg atactcggtg atcctgatgg gttatgcatt
      181 gtgtcatcag gcattgaaac ggcaaaatca ccttcctcga catcaaagaa tgtggtgatt
      241 ctatcgacat ttcccattgt tttctctcct tttaagatta ggaaagaaat cgcctcaccc
      301 cacgaaaggt aggggatctt ggtgcctgag gtactttgta caaaagataa ttcaggagac
      361 gatactccat ataggagata ggactcagta gttcctaatg cctgggctaa cttacttaga
      421 gctttgctac ctggttcgtt tagatctttc tcccagtacc ctatagtaac cccagtcacg
      481 cctgaaagct tacccagttc tacttgggtc agccccttat cttttctaag tctcttaagc
      541 ctgaagccaa ggctttccat cattttctcc tgcaaattga atataaatta ttttagattg
      601 cattgaccta aaaaaaatta tcttgtaatc taaaaatact tagatttggg aggtaaaatg
      661 cgagttgatg aacttgtcca gttttttggt tctgttcaga gggttgccga tttttatggg
      721 ataacccgcg aagctattta catgtggcgt aagcgtcctg gtgaaatagt tccgaagggg
      781 agggctgcgg aagctgctgc atactccaag gggaaattat ctttgaaccc agaactttac
      841 aaaaagaagg ataccactca gggcgaagga aagggggatt catgaaaatc aaacatgagc
      901 acatccgcat ggcgatcaat gcctgggcct atccggacgg tgagaaagtt cctgcagctg
      961 aaatagcccg gacttatttc gagctgggga tgacgttccc ggaactgtac gacgacagcc
     1021 atccggaagc cctggctcgt aatacccaga aaattttccg ttggctggat aaagacaccc
     1081 ctgatgctgt tgaaaaaatt caggctctgt taccggcgat cgaaaaggca atgccgcctc
     1141 tgctggtggc ccgtatgcgc agtcacagct ctgaatatta ccgtgagatc gtcgaacgga
     1201 gggatcggct ggtgaaagat gtggatgatt ttgtcgcagc ggcgatcgcc tggggcaccc
     1261 tgactaacag tggtggtcag cctggtaatg ctgttgtcgt gcattgacca acaatattca
     1321 tgccggattt cttccggatg ttcgagggta aagttcggta tcagatgagg tgagtatggc
     1381 taatgcctgg ctcagattgt ggcatgacat gccaaatgat cccaaatggc gaaccattgc
     1441 cagggtctca ggacagccaa tcgcaacagt gatggcggtg tatatccacc ttctggtgag
     1501 tgcgtcacga aatgtcacga catgtcacgg cgtgtcacta cgtggtcaca ttgatgtcac
     1561 gacggaagat ttagcaagtg cgcttgatgt gacggaagac gtgattgatt caattttgca
     1621 tgcaatgcag gggcgagttc tggatggtga tcttatttcc ggatgggaaa aacgccaggt
     1681 gatgaaggag gataacggta atgtttcgca aaccgcaaaa tccccggcag agcgcaagag
     1741 agcgcagcgg gagcgggaaa gactgcggaa gcagaacact gattgtcacg atgagtcacg
     1801 acgcgtcacg catatgtcac gacaaatcac gacagataca gatacagata aagaattaaa
     1861 ccccacacat aacgcgcgca tgcgcgagag tgctacgacc ggtgagttga atgacgcgtc
     1921 gttgcagaca gccgaacctg aatacctgga cggcctgagc gaaccgatcg ggaaatttcc
     1981 gatgactgga gtctggcaac cgtcgccgga ttttcgacag cgggcagca
//
LOCUS       DABSYT020000278         2007 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.280, whole genome shotgun sequence.
ACCESSION   DABSYT020000278 DABSYT020000000
VERSION     DABSYT020000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.280"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            92..1234
                     /locus_tag="ID725_RS25985"
                     /old_locus_tag="pgaptmp_005197"
     CDS             92..1234
                     /locus_tag="ID725_RS25985"
                     /old_locus_tag="pgaptmp_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HGV7853574.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1708..1808
                     /gene="ykgR"
                     /locus_tag="ID725_RS25990"
                     /old_locus_tag="pgaptmp_005198"
                     /pseudo
     CDS             1708..1808
                     /gene="ykgR"
                     /locus_tag="ID725_RS25990"
                     /old_locus_tag="pgaptmp_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
BASE COUNT          626 a          431 c          408 g          542 t
ORIGIN      
        1 aataaagcca gaactcttgg ttccaaagct ggcaacaact aaccagttaa aacatacgca
       61 tcaaaatcag ataaacattt gaggtattgt tatgacaaat aaacacccat cattgttttc
      121 tccttttatg ctgacagaaa aaataaagct acgtaatcgt atcgttatgg ctcctatgac
      181 gacctggtca gcgaatcctg atggaaccat ctctgagcaa gaactcgaat tttataaacg
      241 tcgctcacaa aatgttggtt tagtaataac aggatgtacc tatgtcacgc caagcggcat
      301 tgggtttact catgaatttg ccgcgtatga cgaccgtttc attaacagcc ttgaaaagtt
      361 agcagcggca gccaaaagtg gcggtgctcc ggctattttg cagattttcc acgccggtaa
      421 taaagccatc ccggagcttg taccaaataa cgatgttatt agtgccagcg catcaagcgt
      481 aaaatctggt gactttatga agagagtggt acaaagcagg gaaatgactg aaaacgaaat
      541 tcaggaaacc attagagcat tcggagatgt cacaaaaagg gcgattaaag caggttttga
      601 tggcattgaa cttcatggtg cgcacggatt ccttctacag aattttttct cccctctatt
      661 taaccagcgt aatgaccgat ggggaggaga tctcgaagga cgtatgcgct tcccgctggc
      721 cgtattacag gaagtaaaaa atgtagtcta tgaatatgcg acaaagccct ttgccatcgg
      781 ttacagaata tctcctgaag aatctgcaac aggggggctg cggatagagg atacttataa
      841 actccttgat cggctcattt catccggtat ttcgtatata catacctcgc tggtcagtat
      901 taacgatagc taccctgttg agtcgccaaa tggcccccga actattgaat tgattttaaa
      961 tcatattgca ggcagagtgc cggtaatcgc tgcaggtaaa atacgcacac cgtctcaggc
     1021 tcaagaagca atttccactg gcctgccact tgttgcgatc ggtaaagggc tggtgataaa
     1081 tccagaatgg gtgactttag ctgaatcagg tagaggccat gaaattcaaa caacccttaa
     1141 tccacaactt gttcctgaat taaccattcc ggataaacta tgggatcaaa ttcaggcatc
     1201 gaaaggaact ggctggtttc cgttaatgga ttaaatatct attactctcc cgagagtaaa
     1261 actatcaaat caatgagtaa ccgatacagc ctcacaaatt ctttcagata attaacccgt
     1321 cactgcggca cgttctctca taaggcgtgc ctgcagtgta atctagtatg tatccggcat
     1381 agttgacggt ttaatagagc tcaattcctc gcccaggtta ccaacaacga tatcttgtat
     1441 aacaccccag gagatacgac attatgatga gatgcgatat gctcctcagc gccaatcaac
     1501 gccaggactg ggccagtctt gagatcagac tcattcccgg aactgacttc aggcgacata
     1561 tatcaatgac gttggggcaa taaaagatgc ctgatgtaat acatctattc aaatttgtga
     1621 tacatgtcaa aaaattggtt gaccaaactc gttatttata taagggcact tacgaagtgc
     1681 gctctttttt aaagcgagga agtaccaatg aaagagaata aagtacaaca aatcagtcat
     1741 aaactgatta atatcgttgt tttgtcgcaa ttgtagaata cgcctattta tttctccatt
     1801 tctattaata acggcaataa actgttcact tcagtgatat ttaaaatatg catcctctcc
     1861 ctttttgtaa gtaattatta tatccgtggg agaggaatac acgctgtcag gtaatcaatc
     1921 atactgcgat aaatcatcgg ccagtaaagt ggagataacc tccattctcg aaaaatccat
     1981 actctcagcg aaactatcat caatcac
//
LOCUS       DABSYT020000279         2000 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.281, whole genome shotgun sequence.
ACCESSION   DABSYT020000279 DABSYT020000000
VERSION     DABSYT020000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.281"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(297..1291)
                     /locus_tag="ID725_RS25995"
                     /old_locus_tag="pgaptmp_005199"
                     /pseudo
     CDS             complement(297..1291)
                     /locus_tag="ID725_RS25995"
                     /old_locus_tag="pgaptmp_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1285..1356
                     /locus_tag="ID725_RS26000"
                     /old_locus_tag="pgaptmp_005200"
                     /pseudo
     CDS             1285..1356
                     /locus_tag="ID725_RS26000"
                     /old_locus_tag="pgaptmp_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            1463..>2000
                     /gene="nleH1-2"
                     /locus_tag="ID725_RS26005"
                     /old_locus_tag="pgaptmp_005201"
     CDS             1463..>2000
                     /gene="nleH1-2"
                     /locus_tag="ID725_RS26005"
                     /old_locus_tag="pgaptmp_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HGV7853575.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEE"
BASE COUNT          503 a          416 c          460 g          621 t
ORIGIN      
        1 ttggctgtat gtttgatgac aaaaaattag cgcaagagga caaaaaatca ccttgcgcta
       61 atgctctgtc tcaggtcact aatcacatct aactcattga ttaattgtga ctctaaatgt
      121 agtgttttgt cgcgttttgc agtctatcat ttaatcaatt gtataaataa cataatgatt
      181 tttaatgtgt ttttgtgatt ttatcctcct tctgacatgg aagttgttcg ttcaaaaatc
      241 ttattaaata tcaagttgtt aaatctggta agtcattatt ggtcaaaata aaatcattat
      301 ccgatttcaa ttttgtccca ctcccgcccg cgactgtccc tataatgcgc cgccattgaa
      361 tcagatttat gtccaagaag acgttgtgca aacttgtagc caatctggtt ccgatatagc
      421 ctcgcggaca gactacgtag ttcatgaaat gtcggtgggt ctccatcaaa cgagatcccg
      481 gatgcatttc gcgcctttgt taaataccgt gatactgtgg ctggagaaag tggttcgtga
      541 taggttgatg cgattattgt ttcactgctg ctggcctccc tgcatttctg tagtgtatca
      601 accagtgaga tgtcgagtgc gtcaatggtt agagccagcg gaatggcaat tttggctcct
      661 gttttactct gtccaatgtg aagatgacca tcgtttatgt ctgaccactt cattctgcac
      721 aaatcgccca ctctctgccc tgtaacgaca gctaaatcca ttgacagcct cagccagatg
      781 gggaggtgct cagcagcatg gtaaatagcg acatactcat tagctgtcag ccttgagcgc
      841 cttacttctg actttgctgc acgggttgtt gttactggat tcgttgccac atgcccctcg
      901 gctattgccc ctcgaaaaac gtcaacaagg gttgacctga ttactcttgc ggaaaccgct
      961 ttaccttctg cgacgtaggt gtttagcatt gctgccacct ctttcgttga tatgtcagtg
     1021 agcggtttgt ccggcaattt tcttcgaatt gccctgattt tgctggcgta gtcgagtaat
     1081 gttttcggct tgctccccct ctagctgagg attgtttcat atcgatcaag ccacgtatga
     1141 agagtgattg agtcagtgcc tttaaccctg tccagcaatg attcgtgccc actttcgctg
     1201 aaaagctcaa tgttggtgtg aatgcttctg aaattgctac tcttctgtct cttcccagcc
     1261 cgaactcttt tttcgtcctc gggtcagtgt gtagtaagac cgctgcaaat cctgcgtgct
     1321 gacgggaagt gggaaaatat tggcgaaatg aagtaacccg atagcttcac aaaaaccgga
     1381 gtccggctcc ggtttttgtt gtcatgtatg ggggatgtct gttaggaata attagatagg
     1441 tttattttga aggttgaaat gtatgttatc gccctcttct ataaatttgg gatgttcatg
     1501 gaattcttta accagaaacc tgacttcgcc tgataatcgt gttttatcct ctgtaaggga
     1561 tgctgctgtt cactctgata gcgggacgca agtaacggtt ggcaacagaa catatcgtgt
     1621 tgtggtcact gataataagt tttgcgttac aagagaaagt catagtggtt gttttactaa
     1681 tctgttgcac aggttgggat ggcctaaggg agagattagc agaaaaattg aggctatgct
     1741 gaatacatcg ccagtgagca cgactataga aagaggctct gttcattcga acagacctga
     1801 tttacctcca gtggattatg cgcagccgga gttacctcca gcggattata ctcaatcaga
     1861 gttgccgagg gttagcaaca ataaatcacc cgtgccaggt aatgttattg gtaaaggtgg
     1921 taatgctgtc gtgtatgaag atatggaaga tacaacaaaa gtgttgaaga tgtttactat
     1981 atctcaaagc catgaagagg
//
LOCUS       DABSYT020000280         1997 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.282, whole genome shotgun sequence.
ACCESSION   DABSYT020000280 DABSYT020000000
VERSION     DABSYT020000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.282"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..302)
                     /locus_tag="ID725_RS26010"
                     /old_locus_tag="pgaptmp_005202"
     CDS             complement(<1..302)
                     /locus_tag="ID725_RS26010"
                     /old_locus_tag="pgaptmp_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HGV7853576.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKG"
     gene            complement(937..1398)
                     /gene="yibG"
                     /locus_tag="ID725_RS26015"
                     /old_locus_tag="pgaptmp_005203"
     CDS             complement(937..1398)
                     /gene="yibG"
                     /locus_tag="ID725_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGV7853577.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIYKQLRYLEKSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(1410..>1997)
                     /locus_tag="ID725_RS26020"
                     /old_locus_tag="pgaptmp_005204"
     CDS             complement(1410..>1997)
                     /locus_tag="ID725_RS26020"
                     /old_locus_tag="pgaptmp_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGV7853578.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          584 a          421 c          329 g          663 t
ORIGIN      
        1 cccttactta ataaatagca tgtataggat tcggaaagtc cagcatctat tttgtatctc
       61 attatattag ataaggatgt gagaaattcc ttgttatcat tcgcagccat atatgccagt
      121 ccgagttcag cctctgcggc cttacaggca aaatagctta gtgaacacgc ttttcgtccc
      181 tctttagtac tggctgatat tagtgcccat aatattgctt tgtctgccaa atctaactct
      241 ctctgaaatt gtcttgcagt aactgcactc gcagaaatag agaaaaaaat acaattgtta
      301 ataaccttgc catatttttt agtccgtaac tgttaatgtg ctatctggct ctgaaaatat
      361 taactcattt aatttttgca tgtcattaga taaaccagtt atacaaccct cggaacagaa
      421 tcgaggtcct aaagacggac caaaagcgtt taaacaacta tgtattgaaa atccattgcg
      481 attataagtt cctgtattaa ctgacggtac taatcgtatg ccgtcaggtt ttctattgct
      541 tgctcccggg tcattaacat tccaactcca taggccttga gatattggcc atctatttat
      601 aaatgtgtac aacggggatt atttttacat tgcatattat taccagaagc gactggaata
      661 ctaagcacat cataaaaatt atcatcacat ggtatatctg aaacacacga aagcatacct
      721 aaagttatgc tgtataaaca ggtggctaat cccaagggga tcgattccag aaatcgggtt
      781 caacggatac tgataaaaat tccacctacc ttccagccca atcggagcct gagtgttata
      841 ccgcccctgc tgcggttaat gttaccgata atagttatag tacagccctg attcatcatc
      901 atactactgc tgcggcagtc tgatgaacta ttgcttttac ggattctcct tattcttcct
      961 tctatccatt cgctttagct ttttgagctc ttgaagtttg ctctttgcat caatattacc
     1021 gttatttaca gcgaattcca tccaataaat agctctattc aaattataat attctgataa
     1081 tgttggattt gtatctgata aaaagacccc ataattaaac tgcgctgtaa cattcccctg
     1141 agatgctgat ttttctaagt atcgcaattg tttgtaaatg ttatttttag taaagcagta
     1201 atattgatat agtcgaaatg atgcctctga atcaccatgt tgtgatttat acttcagttt
     1261 catcaacttt tcttcactta aatcataggt cattgctgta gaccccatca tactttcgtt
     1321 ttgttttatt ttcacatcag caccatgcaa ttgacaaata aaagagaaat aaaaaaatag
     1381 taacaagcat gctttcatac atctacaacc tatcttatat cactgggttt ttctggcgta
     1441 ccttgcatat tagaaatggg gtgcccagat ggcccatatg ttatccaatt accaaggtct
     1501 tttgttgtta tagccattag atccttattt gaattaaggt tcgttggtgc tgttagcact
     1561 gtatccattt ttaacttacc attagttcct cgcccccacc acgtccatag cgtgtcaggt
     1621 cctattacat ttgtatgtaa ctctttagct aattggctag caaatgaatt ttgtccttta
     1681 cctgtattac aagaaaataa ccaaactgtc atcccatctt tataattccc atcaaattta
     1741 attagatatg ctagatcttt agctgtcatg atactgcgcg ttgcggattc aatagatgtg
     1801 ggggtaccat gccccccgat tgtgaaaacg cctgggatat taatctcatc agctacagaa
     1861 tggataagat cactttcggg gtataaattt atatcaacca aaccttgcgg atctacattt
     1921 atgaccggat tcaacggata ctgataaaaa ttccatcccc ccttcagtcc aatcggatcc
     1981 tgagtgatat atcgccc
//
LOCUS       DABSYT020000281         1907 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.283, whole genome shotgun sequence.
ACCESSION   DABSYT020000281 DABSYT020000000
VERSION     DABSYT020000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.283"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1837)
                     /locus_tag="ID725_RS26025"
                     /old_locus_tag="pgaptmp_005205"
     CDS             complement(<1..1837)
                     /locus_tag="ID725_RS26025"
                     /old_locus_tag="pgaptmp_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HGV7853579.1"
                     /translation="MKKKIILVVFLLLILVVCLIGGNYLGGYAALKYSGLNIDMLQWN
                     TFNNVITQFSGKPEYKKLVAITWAGFAAPCAIFIGFVVIVIVGLMPKKVIYGNARLAT
                     DMDLAKSTFFPTDKELKEARLKNSKPYCFPPILIGKQFKGRFKNKYIYFFGQQFLILY
                     APTRSGKGVGIVIPNCVNYPDSMVVLDIKLENWFLSAGYRQNVLGQECFLFAPAGYAE
                     NQQEAKKGNIRSHRWNPLDCVNRSDIQRSGDLEKIAAMLIPASDDPIWSDSARKLFCG
                     LALYLLDKERFHLQQKKKGVADVPDVLVSMSAIMKLSVPESGQKLSAWMGTEIDQKEY
                     LSDETKSLFREFMAAPEKTQGSIITNFSSPLSIFKNPITAAATNASDFDVRMVRKKPM
                     SIYLGLTPDALITHSRLVNLFFSMLVNENTKELPEQNPELKYQCLVLLDEFTSMGKSE
                     IIEKGVGFTAGFNLRFVIILQNEGQGKKDDMYGTHGWETFVENSAVVLYYPPKAKNEL
                     AKKISEEIGVRDMKIIKKSDSRSGGKGGSSRSRNHEIVSRAVLLPEEIVELRDYKNKM
                     GNMAVREIVMSEYTRPFVANKIIWFEEEEFKKRVDIAKENPVEMPV"
BASE COUNT          593 a          429 c          309 g          576 t
ORIGIN      
        1 atacaggcat ttctaccgga ttttctttag ctatatctac tcgtttttta aactcctcct
       61 cttcaaacca gattattttg ttagctacaa atgggcgagt gtattcactc attacgattt
      121 cacgaacagc catattaccc attttatttt tataatcccg aagctcgaca atctcttctg
      181 gcagtaatac agcacggctg actatttcat gatttcggga acgagaacta ccacctttgc
      241 caccactccg tgagtcagat ttcttgataa ttttcatatc cctgactcca atttcctcag
      301 agatcttttt cgccagttca tttttagcct ttggcgggta ataaagaact acagccgagt
      361 tttcaacgaa agtttcccaa ccgtgagtgc catacatatc atctttttta ccctgccctt
      421 cgttctgcaa aataataacg aatcgcaggt taaaaccagc ggtaaagcca acaccttttt
      481 caataatttc agacttaccc attgatgtaa attcatcaag aaggacaaga cactgatatt
      541 tcaactcagg attctgttct ggaagctcct ttgtattctc attcactagc atagagaaaa
      601 acagattcac aagccgtgag tgagtaatta atgcatcagg agtcagccca agatatatcg
      661 acataggctt tttcctgacc attcgcacat caaaatcact tgcattagta gctgctgctg
      721 tgattgggtt cttaaaaata ctcaatggag acgagaagtt ggtaataata cttccttgtg
      781 tcttctccgg cgctgccata aattcacgaa acagactctt tgtttcatca ctcaggtatt
      841 ctttctgatc tatttccgta cccatccagg ctgatagttt ctgaccactt tcagggacag
      901 agagtttcat aatagccgac atggacacaa gaacatcagg tacatcagca acaccctttt
      961 tcttttgctg aagatggaat cgttccttat ccagaagata taaagcaagc ccacaaaaga
     1021 gcttccgtgc agaatcagac caaataggat catcacttgc cggaatcaac atagcggcaa
     1081 ttttttccaa atctccagaa cgctgaatat cagaacgatt aacgcagtcc agaggattcc
     1141 agcggtgtga tctgatatta ccttttttcg cctcctgctg gttttctgca taccccgcag
     1201 gggcaaacag aaaacattcc tgccctaaca cattctgtct gtatccggca gacaaaaacc
     1261 agttttcaag ttttatatca agaacaacca tcgaatccgg gtaattcaca cagttaggta
     1321 taacaatacc aacaccttta ccagaacgcg taggagcata aaggattaaa aactgttgcc
     1381 cgaagaaata tatatatttg tttttaaacc ttcctttaaa ctgtttacca ataagaatag
     1441 gtggaaagca gtatggttta ctgttcttta atcttgcttc tttcaactct ttatctgttg
     1501 ggaaaaaggt tgatttagcc aggtccatat cagtagcaag acgagcattg ccataaataa
     1561 ctttttttgg catcagtccg actatgacaa taacaacaaa accaataaat attgcacatg
     1621 gtgcagcaaa tccagcccat gtaatcgcga ccaatttttt atattcaggt tttccgctaa
     1681 attgtgtaat gacattatta aatgtattcc attgcaacat gtcaatattc aatcctgaat
     1741 attttaatgc tgcatatccc ccaagataat taccgccaat aagacaaaca acgagaataa
     1801 gcaacaaaaa aaccactaat atgatttttt tcttcataga tacccctaca tgtaaagcat
     1861 gtataatatt taatcaccag aagtaattta cattgtgcta agtagac
//
LOCUS       DABSYT020000282         1893 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.284, whole genome shotgun sequence.
ACCESSION   DABSYT020000282 DABSYT020000000
VERSION     DABSYT020000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.284"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            151..>1893
                     /locus_tag="ID725_RS26030"
                     /old_locus_tag="pgaptmp_005206"
     CDS             151..>1893
                     /locus_tag="ID725_RS26030"
                     /old_locus_tag="pgaptmp_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGV7853580.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QVITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADAGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLAQFRADLSVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYGTQYNTIYGAYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRMATAILNAAGRTSAFISGKA
                     PEIKPSPGGNTPSGPSADTSVRTISLLPAAGEAAAQGWSIKDGGIQLSDGVFKITKQS
                     NKTWSLTHPVDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYLYTDAPVPDGVAMTG
                     TGNPFLMSYFTQTTDGRVNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDS"
BASE COUNT          435 a          491 c          556 g          411 t
ORIGIN      
        1 tcgtgtggat tttaaccagg gtttatcagg ctgcgcaact gcgtggcctt ttttcatttc
       61 ttgggctgta gtccccgtgt gtcattcagg cttccggact acagcccact ccatatctga
      121 tttaatacac tatcccggcc gggaggaata atgacattta aacattatga tgttgtcagg
      181 gcggcgtcgc cgtcagacct tgcggaaaag ctgacacaca aactgaaaga gggctggcag
      241 ccgtttggta gtccggtggc cataacccct tataccctga tgcaggtgat tacagcagaa
      301 ggtgatgtgg tggtcagtgg tgcaactgag ccggattggt actacgtcat cgtactggcc
      361 gggcagtcca atgccatggc ttacggtgaa gggcttccgc tgccggattc atacgatgct
      421 ccggatccgc gcattaaaca gctggcgcgc cgcagtacag ttacgccggg tggggctgcc
      481 tgcagatata acgatattat tccggccgac cactgcctgc atgatgtgca ggatatgagt
      541 acgctgaatc atccgaaggc agacctgagc aaagggcagt acggctgtgt cggccagggc
      601 ttacatattg ccaaaaaact gcttccgtat atcccgaata acgcggggat cctgctggta
      661 ccatgctgtc gtggtggttc ggcattcacc cagggcgcgg aggggacatt cagtgcggac
      721 gcgggggcca gccaggattc ggcgcgctgg ggtgtgggta aaccgttata tcaggacctg
      781 attgcgcgca ctaaagctgc attacagaag aacccgaaaa atgtgttgct ggcggtgtgc
      841 tggatgcagg gagagtttga catgagcgcc gccacccacg cacagcaacc tgcgctgttt
      901 acagccatgc tggcacagtt tcgtgctgac ctctccgtgt ttaacgcgca gtgccatggt
      961 ggcagtgctg cagatgtgcc gtggatttgt ggtgacacga cgtattactg gaaaaatacc
     1021 tacggcaccc agtacaacac catttacggg gcgtacaaaa acagggagag tgagggcgtt
     1081 tattttgtgc ccttcatgac agacggtaac ggcgtcaata ccgccactaa cgcgccggca
     1141 gaagatccgg atattccggc atcaggatat tacggtgcgg catcgagaac gaatggaaac
     1201 caggtatcat caaaccgccc gacacatttc agttcatggg cgcgcaggag cattattccg
     1261 gatcgtatgg caaccgctat tctgaacgca gccgggcgca cctcagcctt catcagtggt
     1321 aaggcaccgg aaatcaaacc ctcgcccggc ggcaacacgc catcgggtcc gtctgcagat
     1381 acgtccgttc gcacaatctc cctgctgccg gcagccggag aggctgctgc gcagggctgg
     1441 agcattaagg atggcggaat tcagttgtca gatggtgtat ttaagatcac caagcagagc
     1501 aataaaacct ggtccctgac gcatccggtg gatgacgcaa ttaccctgct gacacagggc
     1561 ggcagactga cctgtaagtt ccgcctgtca ggcgcactga ccaacaatca gttcgggctg
     1621 gggatttatc tgtatacgga tgctcccgtt cctgatggtg tggcgatgac gggtaccggt
     1681 aatccgttcc tgatgtcgta cttcactcag accactgacg gcagagtgaa tctgatgcat
     1741 cacaggaaag ccggaaacac gaagctgggg gagttcggcg attacggtaa cgactggcag
     1801 acgctggagc tggtgttcac cgccggcagt gccacggtta ctccgaaact gaatggagtg
     1861 gctggcccgg cattccaggt tataaaagac agt
//
LOCUS       DABSYT020000283         1887 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.285, whole genome shotgun sequence.
ACCESSION   DABSYT020000283 DABSYT020000000
VERSION     DABSYT020000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.285"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            297..458
                     /locus_tag="ID725_RS26035"
                     /old_locus_tag="pgaptmp_005207"
                     /pseudo
     CDS             297..458
                     /locus_tag="ID725_RS26035"
                     /old_locus_tag="pgaptmp_005207"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="ID725_RS26040"
                     /old_locus_tag="pgaptmp_005208"
     CDS             442..507
                     /locus_tag="ID725_RS26040"
                     /old_locus_tag="pgaptmp_005208"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HGV7853581.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="ID725_RS26045"
                     /old_locus_tag="pgaptmp_005209"
     CDS             623..880
                     /locus_tag="ID725_RS26045"
                     /old_locus_tag="pgaptmp_005209"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGV7853582.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ID725_RS26050"
                     /old_locus_tag="pgaptmp_005210"
     CDS             complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ID725_RS26050"
                     /old_locus_tag="pgaptmp_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HGV7853583.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
     gene            complement(1562..>1887)
                     /gene="nleH1-2"
                     /locus_tag="ID725_RS26055"
                     /old_locus_tag="pgaptmp_005211"
     CDS             complement(1562..>1887)
                     /gene="nleH1-2"
                     /locus_tag="ID725_RS26055"
                     /old_locus_tag="pgaptmp_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HGV7853584.1"
                     /translation="EKIYNDNGNVIGIRMNKINGESLLDIPSLPAQAEQAIYDMFDRL
                     EKKGILFVDTTETNVLYDRMRNEFNPIDISSYNVSDISWSEHQVMQSYHGGKLDLISV
                     VLSKI"
BASE COUNT          585 a          416 c          338 g          548 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccacgagg
     1021 catttcattg ctcgtagatc accatatagc tctgaagagt gatgcggaca tagcagatta
     1081 taaactttac atttcgaatc ttctttgcct tgtctacttt cctcaatttt tactttcaat
     1141 gaatgcagaa aaaaaagatc cctgatataa ctatccctgt cctctatcgt cattcccgcc
     1201 ctcagcgctc cgacaacctc atcagtattt tcatttctca cctcaaatac actatttctc
     1261 ttatccatca gcccacttac aaaagcactt agctcttgaa aaatttcctc aagctcatta
     1321 ttatttctgg cacatgaata tatagagacc ataacatcaa gtttatcttg taattcagga
     1381 gaaagaaagt gattatgatt aagctcactt actgattccg gagtcgaagg gttacctctt
     1441 cctgatacat acatccatga ataaagattt gctgaagaac cacttgttgg taacatatca
     1501 aaaccccctt aacaaataaa tccataaata ttattgcaac taatatatct ggataaaaat
     1561 attatatctt acttaatact acactaataa gatccagctt tcctccgtga taagattgca
     1621 tgacttgatg ttcactccat gaaatatcag aaacattata agatgatata tctattggat
     1681 taaattcatt tctcatacga tcatataaaa catttgtttc tgttgtatca acaaaaagaa
     1741 ttcctttttt ctccagtctg tcaaacatat cgtaaatagc ctgttcagct tgtgctggta
     1801 atgatggaat atccaaaaga gattccccat ttattttatt cattctaata ccaataacat
     1861 ttccattatc attatatatt ttctctg
//
LOCUS       DABSYT020000284         1879 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.286, whole genome shotgun sequence.
ACCESSION   DABSYT020000284 DABSYT020000000
VERSION     DABSYT020000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.286"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..938
                     /gene="rfaL"
                     /locus_tag="ID725_RS26060"
                     /old_locus_tag="pgaptmp_005212"
     CDS             <1..938
                     /gene="rfaL"
                     /locus_tag="ID725_RS26060"
                     /old_locus_tag="pgaptmp_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGV7853585.1"
                     /translation="ASMKEIFKAIIENTLLCTIAIPIILRDEKREDVEKIVFFSFISA
                     LGLRCFSELITYYKDYQQGIMPFADYRHRSISDSMVFLFPALLNLWLIKSAKYRISFV
                     VLSVIFIFLILGTLSRGAWLSVLVIGLIWILMFKQWKLLLVGVMVAIIALSVIFTHKE
                     MTAKLTYKLQQTNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKDVYNKRVIDYPE
                     WTFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCAILKECIKNGVKNKYRSPYNAYYII
                     LLSFIGYFVIRGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(974..>1879)
                     /locus_tag="ID725_RS26065"
                     /old_locus_tag="pgaptmp_005213"
     CDS             complement(974..>1879)
                     /locus_tag="ID725_RS26065"
                     /old_locus_tag="pgaptmp_005213"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGV7853586.1"
                     /translation="DPLPYSQRILNIAHDFPITKESVIVVHNSMKLYTQIRKRAPQAR
                     VVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGIDLETYQSNFQP
                     ITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYTEMSKS
                     DKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQEPFCMVAIEA
                     MGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTAVAKQGQ
                     DFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          573 a          343 c          334 g          629 t
ORIGIN      
        1 gtgctagcat gaaggaaatt ttcaaagcta ttattgagaa tactttacta tgcacaatag
       61 caataccgat catattgaga gacgagaaaa gagaagatgt cgaaaaaatc gttttcttct
      121 catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat tataaggact
      181 atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct gactcgatgg
      241 tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa taccgcattt
      301 cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta tccagagggg
      361 cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa caatggaagt
      421 tactattagt aggagtaatg gttgccatca ttgcattgtc ggttattttc acacataagg
      481 agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat cgctatgcaa
      541 atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt ggttatggtt
      601 acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat ccagaatgga
      661 cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt ggcactggtt
      721 tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag tgtataaaaa
      781 atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc ttactatctt
      841 ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat ttattaggcg
      901 tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag gctgcataat
      961 gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt tgagttacgc
     1021 catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact gctgttaatt
     1081 caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc ataggttctt
     1141 ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca cgcgtactca
     1201 ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat ggttcctgga
     1261 actgggaagg aatgacgact aaatctgcca gtgggtaata acaatgcatc ttttcaggcg
     1321 gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc ctgacattgc
     1381 gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc acaactaatt
     1441 ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc aatatgcctt
     1501 tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggggag atattaagtt
     1561 cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt ccatttggta
     1621 caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag tataaactag
     1681 gcactatcat tttaacattt tgctcaagca attttggttc aaatgcatta tgcatatgta
     1741 taaccactct tgcctgagga gctcgcttac gaatttgagt gtatagcttc atgctattat
     1801 gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc aatattctct
     1861 gtgaatatgg aagaggatc
//
LOCUS       DABSYT020000285         1867 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.287, whole genome shotgun sequence.
ACCESSION   DABSYT020000285 DABSYT020000000
VERSION     DABSYT020000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.287"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..1386)
                     /locus_tag="ID725_RS26070"
                     /old_locus_tag="pgaptmp_005214"
     CDS             complement(<1..1386)
                     /locus_tag="ID725_RS26070"
                     /old_locus_tag="pgaptmp_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGV7853587.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMS"
     gene            complement(1434..1643)
                     /locus_tag="ID725_RS26075"
                     /old_locus_tag="pgaptmp_005215"
     CDS             complement(1434..1643)
                     /locus_tag="ID725_RS26075"
                     /old_locus_tag="pgaptmp_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGV7853588.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(1739..>1867)
                     /locus_tag="ID725_RS26080"
                     /old_locus_tag="pgaptmp_005216"
     CDS             complement(1739..>1867)
                     /locus_tag="ID725_RS26080"
                     /old_locus_tag="pgaptmp_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGV7853589.1"
                     /translation="DLQRVFTPDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          370 a          638 c          459 g          400 t
ORIGIN      
        1 agacattgac ggcgttttat gcgcgatttc atctatcgtc agtgcatact ggcgcgcaga
       61 ctccctggcc tgctcctgat tctggtacag cgtgtaccat gctgcagccc ccagcatcac
      121 cagtccgggt acgccaccaa ccagccccag cgcaccgctc atcagacgtg agcccaccgc
      181 cgttgtactg ttcagcgcat tctgggcggc gcttctggca gcaatatttc tgttcaggcg
      241 ttcctgtgtg gccgccagac gggcctctgc tgcaatctgc atctccgtcc cgcgggctgc
      301 cgccacggcc tgctgagcac ggtacacggc tgcccttgcc cgcgccgtgg caatctgcgt
      361 tcccctgaac tgtgcttccg ccagtgcaac ttcattacgt gcagccgtca caagtcctgc
      421 cgtggcagac atcgctccgg aggccatatt gccaaagtac cgggcaaccc cgacggcaac
      481 cagcgcgccc acggctgttg ccacattatc aatctgtccg gcaacaccgt tcagcatgcc
      541 ggagagcgtt tttgtcaccc cgctggcctc attcgcaccg cccacccagg ccataaaggc
      601 gttttccacc tttgtgatac tactggaaac cgtttccggc atggccgcat attcatcacg
      661 taatatcccc agctggctga ttaacgcggg gaccacttta tccgctgtca gttttccgtc
      721 atccgccatt gccttcagat ctttacgggc cacgcccata cccgcagcca gtgcacgtac
      781 gatccggtca ccactttcat tgaccgaatt aaactcctca ccacgcaata caccctgcgc
      841 cagcgcctga ctgaactggg tgatcaccga gcccgcctct gccgtactgg caccggagat
      901 tttcagccct gtcgaaatgg cctccgtcac cttcagcaca tcatcagcac tgtaaccata
      961 ttcacgcatc gaggcagccg aacgggcaaa cagggccgca ttatccgaaa atgcggtgcc
     1021 tgtccgctgg ctgatatcca tcagcacttt ctgtgatgac gcaaattcat cggatgactg
     1081 tgatgcctgt ttcagacggg cattcacgga gctccattca tccgccagcg aaatcaggtg
     1141 tccggtggca aaggcaccgg caaacgcacc ggtcattccg acagccgaag cgcggatttc
     1201 cgtcaactgg ctgtgcagct cagccagagc ccggcgctgc tccctggctg ccgcagcagc
     1261 ctgacgtccg ccattctgca gggtccggta atattcactg cccatacggg acgcccgctg
     1321 gatctccgac tggaatgact gtgaatttgc cgaaattttg ataatcagtt cacgtaacgt
     1381 cgccattcac ctttctccgg gcgtaaaaaa accgcctcag cggttctcat cattcatgac
     1441 tgtgctgcaa ggctcagcgc gtcttccagc gccgcaaacg gatccacctc cggcttatcc
     1501 tcatcctcgc cccagcagag catggcgtcc ttcagtgcaa cattcatccc ctgtgccccg
     1561 aaaaccgctt tcacgatctg tgcattacgg atatccccgc gctcatcacc cagcggggat
     1621 atcctgtcga actccatcca catcatcgcc tcgctcgcac tcaggctgtg gcgcagttcg
     1681 gataaggtgc gccccagacg gagcgcaagt cgcatcagaa agcgaatttc cgggcgggct
     1741 acttttttct ggccgactct gcatcagcga tcagttccag tgcctgacgc agcaaccggg
     1801 catgtaccgg accatagacg gccagcacct gctcacggtc gtccggagtg aacacccgct
     1861 gcaggtc
//
LOCUS       DABSYT020000286         1857 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.288, whole genome shotgun sequence.
ACCESSION   DABSYT020000286 DABSYT020000000
VERSION     DABSYT020000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.288"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(86..1498)
                     /gene="pykF"
                     /locus_tag="ID725_RS26085"
                     /old_locus_tag="pgaptmp_005217"
     CDS             complement(86..1498)
                     /gene="pykF"
                     /locus_tag="ID725_RS26085"
                     /old_locus_tag="pgaptmp_005217"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGV7853590.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1506..1700)
                     /locus_tag="ID725_RS26090"
                     /old_locus_tag="pgaptmp_005218"
     CDS             complement(1506..1700)
                     /locus_tag="ID725_RS26090"
                     /old_locus_tag="pgaptmp_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853591.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          468 a          434 c          450 g          505 t
ORIGIN      
        1 ttttttattg cttctggtta tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata
       61 tacaaattaa ttcacaaaag caatattaca ggacgtgaac agatgcggtg ttagtagtgc
      121 cgctcggtac cagtgcacca gaaaccataa ctacaacgtc acctttgtgt gccagaccgc
      181 tctgcagagc cagttcttta cccagacggt agaaatcatc agtagaagtg atctctttaa
      241 ccagctgcgg cacaacgcct ttgctcagta ccaactgatg agccgttttt tcgttggtgg
      301 tcagtgccag gatggtggca tccgggaagt atttacgtac tgcgcgagca gatttaccgc
      361 cctgggtagc aaccacaatc agcggagcat ccagtttttc agcagtttca acggcaccac
      421 ggcataccgc ttcggtaatg cgcagtttac ggttgtcatt gttgaactcg agacggctgt
      481 tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat agaaaccgct tccagcgggt
      541 atttaccttt tgcggattca ccagacagca tcactgcgtc agtaccgtcg aggatggcgt
      601 ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca
      661 tctgggtcgc agtgataacg actttacgtg cacggataca tttttcgatc atcatcttct
      721 gggcgaagat aacttcttct accgggattt ctacacccag gtcgccacgc gcaaccatga
      781 tgccgtcaga ggcttcgagg atttcgtcga agttgttgag gccttcctgg ttttcgattt
      841 tggagatgat gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg atttcgataa
      901 cgtcagaacg cttacgaata aaggaagcag cgacaaagtc tacgccttgt tcgcaaccaa
      961 agatcaggtc ctgtttgtct ttttcagcca gtgctggcag ggcaatggaa acgccaggca
     1021 ggttcacacc tttgttttca cccaggtcgc cgttgttcag cactttacag ataactttgt
     1081 taccttcaat ggcagtaact tccataccga tcagaccatc gtcaaccagt acggtgttgc
     1141 caacagacag gtcagttgtg aaaccttcat acgttaccgc aaccatttcg ctgttgccga
     1201 taacagattt atcagtagtg aaagtaaagg tctgaccagc tttcagagaa acgtcgttac
     1261 cgccttccag tttcatggtg cggatttccg gacctttggt atcaagcagg atagcggcgg
     1321 ttttaccagt tttgctcatc acgttgcgca gattctgaat gcgctgaccg tgttctgcat
     1381 agtcaccatg agagaagttc agacgcataa cgttcatgcc agcgtccagc attttagcta
     1441 acatctcttc agattcggtt ttcggtccga tggtgcaaac aattttggtc tttttcatga
     1501 cagtcttagt ctttaagttg agaaggatgg gagaaacttg ctttctgggc gcactgccgg
     1561 aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa
     1621 aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt
     1681 cgttgagttc tacagtccaa agtgctgaaa ccattcaaga gtcaattggc gcgcattata
     1741 cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc aaaaaatata caacttgtta
     1801 gacaataaat tgttatcaaa gatttaacgt tccagggaaa aggttacgca aaaactt
//
LOCUS       DABSYT020000287         1807 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.289, whole genome shotgun sequence.
ACCESSION   DABSYT020000287 DABSYT020000000
VERSION     DABSYT020000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.289"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..662
                     /locus_tag="ID725_RS26095"
                     /old_locus_tag="pgaptmp_005219"
     CDS             <1..662
                     /locus_tag="ID725_RS26095"
                     /old_locus_tag="pgaptmp_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGV7853592.1"
                     /translation="FRYAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
     gene            666..1082
                     /locus_tag="ID725_RS26100"
                     /old_locus_tag="pgaptmp_005220"
     CDS             666..1082
                     /locus_tag="ID725_RS26100"
                     /old_locus_tag="pgaptmp_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853593.1"
                     /translation="MSQPLSEILTWDDEQWEVFVHDWLIVCKSDDYPWSERLGGAGDK
                     GRDVVGYKSDPNVEGYSWDNYQCKLYKKSLGFSDVVVEFGKLIYFTLNGDYPIPQKYF
                     FVAPYDLSTTFSNLLKNKNELKKAVLDSWDSAISKK"
     gene            1055..1672
                     /locus_tag="ID725_RS26105"
                     /old_locus_tag="pgaptmp_005221"
     CDS             1055..1672
                     /locus_tag="ID725_RS26105"
                     /old_locus_tag="pgaptmp_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HGV7853594.1"
                     /translation="MGFSNFKKITKKIDIPLDDEIKKYIEDFDFSIFYSLPLSLILND
                     IANTHLYFKYFNELYVVRIPPNEIPTYNSKKESVYVNALLQAYSEHGNKTYSSFLELD
                     DPYRRHFNNSRNDFYFASSLEVFVREVFKDDVFKALKCYISSSIEPVFYEDHNYAFIR
                     CNAVLKQAVLTPIAHSVLSKICEANDKKGICHHLVNDGEVIWTVR"
     gene            1672..>1807
                     /locus_tag="ID725_RS26110"
                     /old_locus_tag="pgaptmp_005222"
     CDS             1672..>1807
                     /locus_tag="ID725_RS26110"
                     /old_locus_tag="pgaptmp_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HGV7853595.1"
                     /translation="MVRIYNSSLEVACRMAKVLVAIYPSSLSLERLICFDFILVNLKD
                     F"
BASE COUNT          563 a          289 c          370 g          585 t
ORIGIN      
        1 tgtttcgcta cgcaattgca acgggtaggg cggagtacaa tcctgcggct gatctctcca
       61 gcgctctcga agtacaccaa tccaatcatt tcccattcct aaaagctgat gagatacctg
      121 attttctacg tgccttagag ggttactccg ggagtaagct tgtccagata gccacgaaat
      181 tactgatgat tacgggtgtg agaaccatcg aattacgcgc ggcattatgg caagaatttg
      241 atctggataa cgctatttgg gaaattcctg ctgaaaggat gaaaatgcgt aggccacatc
      301 ttgtgccctt atcatctcaa gcggtagatt tactcaatga actcaagatc atgacaggga
      361 actatcgtta tgtttttcca gggcggaacg atccgaatag gccaatgagc gaagcgagta
      421 taaatcaagc cattaagcgt attgggtatg gaggaaaagt cactggacat ggttttcgtc
      481 ataccctttc tacaatcctg catgagcaag gttttgagag tgcttggatt gaaatccagt
      541 tggctcatgt agataaaaat tctattaggg ggacttataa ccatgctcaa tattttagtg
      601 gaaggaagtc tatgatggac tggtacagta atttgatatt tgaaagacta aaaaggagtt
      661 aatttgtgtc tcaaccactt tcggaaatat tgacatggga tgatgaacaa tgggaggtat
      721 ttgtccatga ttggcttatt gtctgtaaat cagatgatta cccgtggagc gaacgtttgg
      781 gaggagctgg agataaaggt agagacgttg ttggatataa atcggatcct aacgtagaag
      841 gttattcttg ggataattat caatgcaaac tgtacaaaaa aagtttaggg ttctctgatg
      901 ttgtagttga gtttggaaaa cttatctatt ttactctgaa tggtgattat cccatccctc
      961 agaagtactt ttttgtggca ccctatgatt tatctactac attttctaat ttattgaaaa
     1021 ataaaaacga gcttaaaaaa gcagtccttg attcatggga ttcagcaatt tcaaaaaaat
     1081 aactaaaaag attgatattc cattagatga tgaaataaaa aaatatattg aggattttga
     1141 ttttagtatt ttttactctc tacccttatc attgatttta aatgatattg caaatacaca
     1201 cctttatttt aagtacttta acgagctgta tgtcgtgaga atccctccaa atgaaattcc
     1261 aacatacaat tcaaaaaaag agtctgtata tgttaatgca ctgcttcaag cctattcaga
     1321 gcatggaaat aaaacttata gttctttctt agagcttgat gatccataca gacgacactt
     1381 taataatagt agaaatgatt tttattttgc atcttcgctt gaggtttttg tccgcgaagt
     1441 atttaaagat gatgtattca aagcattgaa atgttacatt tcatcttcaa ttgaacccgt
     1501 cttttatgaa gaccataatt atgcatttat taggtgtaat gcagtcttga agcaggctgt
     1561 tctgacacca attgcacatt cagtactatc aaaaatatgt gaagcaaatg ataaaaaagg
     1621 aatatgccat catttggtta atgatggtga agtaatttgg acggtgagat aatggttaga
     1681 atttataatt caagtttaga agtggcatgt cgaatggcga aagtgctcgt cgctatttat
     1741 ccttcttcat taagccttga acggcttatt tgttttgatt ttattttagt aaatcttaag
     1801 gattttt
//
LOCUS       DABSYT020000288         1798 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.290, whole genome shotgun sequence.
ACCESSION   DABSYT020000288 DABSYT020000000
VERSION     DABSYT020000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.290"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(694..1500)
                     /locus_tag="ID725_RS26115"
                     /old_locus_tag="pgaptmp_005223"
     CDS             complement(694..1500)
                     /locus_tag="ID725_RS26115"
                     /old_locus_tag="pgaptmp_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGV7853596.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPNLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(1501..>1798)
                     /gene="ccdB"
                     /locus_tag="ID725_RS26120"
                     /old_locus_tag="pgaptmp_005224"
     CDS             complement(1501..>1798)
                     /gene="ccdB"
                     /locus_tag="ID725_RS26120"
                     /old_locus_tag="pgaptmp_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HGV7853597.1"
                     /translation="KVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVS
                     RELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
BASE COUNT          456 a          482 c          423 g          437 t
ORIGIN      
        1 ccggggcggt tcagtgacaa ttttttagat tgtcacacta aataaaaaag agtcaataag
       61 cagggataac tttgtgaaaa aacagcttct tctgagggca atttgtcaca acaccttctc
      121 tagaaccagc atggataaag gcctacaagg cgctctaaaa aagaagatct aaaaactata
      181 aaaaaataat tataaaaata tccccgtgga taagtggata accccaaggg aagttttttc
      241 aggcatcgtg tgtaagcaga atatataagt gctgttccct ggtgcttcct cgctcactcg
      301 agggcttcgc cctgtcgctc gactgcggcg agcactactg gctgtaaaag gacagaccaa
      361 acatcatggt tctgtgttca ttaggttgtt ctgtccattg ctgacataat ccgctccact
      421 tcaacgtaac accgcacgaa gatttctatt gttcctgaag gcatattcaa atcgttttcg
      481 ttaccgcttg caggcatcat gacagaacac tacttcctat aaacgctaca caggctcctg
      541 agattaataa tgcggatctc tacgataatg ggagattttc ccgactgtct cgttcgcttc
      601 tcagtggata acagccagct tctctgttta acagacaaaa acagcatatc cactcagttc
      661 cacatttcca tataaaggcc aaagcattta ttctcaggat aattgtttca gcatcgcaac
      721 cgcatcagac tccggcatcg caaactgcac ccggtgccgg gcagccacat ccagcgcaaa
      781 aaccttcgtg tagacttcct ttgaactgat ggacttatgt cccatcaggc tttgcagaac
      841 tttcagcggt ataccagcat acagcatgtg catcgcatag gaatggcgga acgtatgtgg
      901 tgtgaccgga acagagaacg tcacaccgtc agcagcagcg gcggcaaccg cctccccaat
      961 ccaggtcctg accgttctgt ccgtcacttc ccagatccgc gctttctctg tccttcctgt
     1021 gcgacggtta cgccgctcca tgggtatttt cagtgttgcc accatcgtct gcagctggct
     1081 gacgtaccag gagtcagaga gcggaaccag ccggtgagtc tgctgaccgg cgggcattct
     1141 ccccgccgtc ctggcagctt tttcggtccg ttgtttcagg gtcgcaagct gcacaaacgg
     1201 atacggaggc gcaagcgaaa aatccccccg cgtcagcgcc agtgcttcat taatgcgtgc
     1261 tccggtgttc cacagtgtgg ccagcagcat cttgcggtgc agattcggga cgtaatggag
     1321 cagggcactc acttccggag ccagcagata ttttggcagt tcatcatgga ccatcgacat
     1381 ctggcgaagt gccagagctg ccggataatc aatggcaaca ggcagcgatg caggctgtcc
     1441 ggcagaatac actgccggta ccatgactgc agactggctg tgtataacgg agcctgacat
     1501 ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag
     1561 atcagccact tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat
     1621 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga
     1681 cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc
     1741 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaacctta
//
LOCUS       DABSYT020000289         1780 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.291, whole genome shotgun sequence.
ACCESSION   DABSYT020000289 DABSYT020000000
VERSION     DABSYT020000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.291"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..676
                     /locus_tag="ID725_RS26125"
                     /old_locus_tag="pgaptmp_005225"
     CDS             <1..676
                     /locus_tag="ID725_RS26125"
                     /old_locus_tag="pgaptmp_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853598.1"
                     /translation="IESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAG
                     RINARLEDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVC
                     RWFGTSDLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEI
                     DAFFCAFREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALRE
                     ELETML"
     gene            673..1578
                     /locus_tag="ID725_RS26130"
                     /old_locus_tag="pgaptmp_005226"
     CDS             673..1578
                     /locus_tag="ID725_RS26130"
                     /old_locus_tag="pgaptmp_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGV7853599.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            1575..>1780
                     /locus_tag="ID725_RS26135"
                     /old_locus_tag="pgaptmp_005227"
     CDS             1575..>1780
                     /locus_tag="ID725_RS26135"
                     /old_locus_tag="pgaptmp_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGV7853600.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASTDLSEAAVRLEALW"
BASE COUNT          435 a          429 c          540 g          376 t
ORIGIN      
        1 aatcgaatcg accgtgacca gcctgcaccg tgaggctgaa gcacagttcc ggcctgagct
       61 ggagaaaatc gtcagcggga ttgaaacagg ttttcgtggc acggccctgt acgccacaga
      121 aaacattgcc ggtcgcatca atgcccgcct ggaggatgag ggctttaccg taaaaatcag
      181 ttttccggca gtcagccagt tacagacccg gctcgcggta aaaataaatc tgagtgcgct
      241 tatggaggaa agaacggaga ccgtcacccg tcgccgtcgg cagagtggcg tatggggaac
      301 cgtttgtcga tggtttggca ccagtgacct gggctgggaa aactatgacg aggatgtgag
      361 tcgcagcgtg atcaatatca acaaggtcag agaggaagtt atgtcactga cccgggcata
      421 tttcggggag ctgcaggcat ccattgagca ggatattaac cagcccgtcc gccaggaaat
      481 cgatgccttt ttctgcgcat tcagggagaa agttgaacaa ctgcgtaaca cgctgattca
      541 gagctctgaa gatcataaac gcgatcagca ggcgcaggaa cggcttaccg ggcgacttca
      601 ggcattaaat gaaagggttc ctgagctcat tactgacagt aaggcgctga gggaagagct
      661 ggagacaatg ctgtgacttc accatttatt caacaaattg ctgataaccg ggtatgtcag
      721 gtactcacct gtcttcctga aaaatttgtg gttgatttcg caaacggaat cgatgttgca
      781 caggagcata tccgcacggc cggagagcgc acgttcttcc ggcgtttaaa agaggggctg
      841 accggcgaag gcgctgcacg gcagaatgcc atcaatgcgt cgcttgcaca gggcgttgag
      901 gcgtccctgc gctggctgac ggagatgaca acgtcacttg ccaccacaaa ctacgcgatt
      961 acccgggtaa acgacagggt cagttcactg gtcagtgata cagccaggct ggcgcattat
     1021 tccgcagata cgcgggagca gttactcacc ctggccgatc aggttcacca taaactgaat
     1081 catcttgaag aaaaactcca ccgtgttgac caggttcagc gggcgcagtt acatcttgag
     1141 cagatattct catggtggag cgccgggcga tatgcgtcat tttcccctgc cggacgttgt
     1201 tatgtggcac ttgaagagct tcgctggggc gcgtttggtg atgtgatacg tcagagcgaa
     1261 acaggccagg ttaaccagct actggatatt ctcagacata aagcattaac gcagatggca
     1321 caggagagtg gcggtagtgc aaccgtgcgt ctgaacactc tggactggct tggtggtcag
     1381 ggccgggagc aggcagataa cgagtggcat gacgcgatta actggctggg agactggtgc
     1441 agtgaagagc agcatccggt gatctggtca accacgcagg ctgcagaaca cttaccggtt
     1501 cgcatgcctc gcctctgctc tgcagaacgc ctctctgaaa gtatggtcga tgaaatattt
     1561 cagaaggggg ctgcatgagt acagaaatga aaacggggct ggtgctgtcc ggtggcgggg
     1621 cggtgggcgc ttatcaggcg ggagtggtta aggcactggc agagtgtggt acacagatca
     1681 gcatggtttc aggggccagc attggcgcat tcaatggtgc cattatcgcg gcctctaccg
     1741 atctgtcaga agctgccgta cgcctggagg cgctctggga
//
LOCUS       DABSYT020000290         1763 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.292, whole genome shotgun sequence.
ACCESSION   DABSYT020000290 DABSYT020000000
VERSION     DABSYT020000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.292"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..73
                     /locus_tag="ID725_RS26140"
                     /old_locus_tag="pgaptmp_005228"
     CDS             <1..73
                     /locus_tag="ID725_RS26140"
                     /old_locus_tag="pgaptmp_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853601.1"
                     /translation="ENGYLLKNEIIRSLSSRPTDFLP"
     gene            complement(271..777)
                     /gene="espH"
                     /locus_tag="ID725_RS26145"
                     /old_locus_tag="pgaptmp_005229"
     CDS             complement(271..777)
                     /gene="espH"
                     /locus_tag="ID725_RS26145"
                     /old_locus_tag="pgaptmp_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HGV7853602.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(808..1698)
                     /gene="escQ"
                     /locus_tag="ID725_RS26150"
                     /old_locus_tag="pgaptmp_005230"
     CDS             complement(808..1698)
                     /gene="escQ"
                     /locus_tag="ID725_RS26150"
                     /old_locus_tag="pgaptmp_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HGV7853603.1"
                     /translation="MKINDFYLPLLPVIGTGRLYITSKGHACHAYFREVSGHGIRFTL
                     TYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTALPEGDVSVDD
                     VQFTMLNKDIYPVIENNNGENRLNVIILETTVQSLQYLINDNWQLVPHSNALFFDGYI
                     VPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINTPLATVNLSDNNLSVTDVLAAD
                     LLSALSNETVMNRIYCAIGTVHIDIHMLRNVKKDDIINSDGYHLFGGCQLIRNNTTIA
                     YGSIVKINEDFYFTVSIVCD"
     gene            complement(1688..>1763)
                     /locus_tag="ID725_RS26155"
                     /old_locus_tag="pgaptmp_005231"
     CDS             complement(1688..>1763)
                     /locus_tag="ID725_RS26155"
                     /old_locus_tag="pgaptmp_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HGV7853604.1"
                     /translation="DYVFDKLKEIFPDEAIEFTIEYEN"
BASE COUNT          555 a          373 c          297 g          538 t
ORIGIN      
        1 ggaaaatggt tatctattaa agaatgagat aattagatcc ttgtcttcca gacctacaga
       61 ttttttacct tgataatttc tgcgctggat gggatcatcc attccgttta acatactctt
      121 catcacaggg acctctgatt tctggccgct aacgtctctg aagattcagc ctgtattatc
      181 taaaagcaat gtaatatcag ttaaaaagcg gaaaaactcc agaattataa ctattctgga
      241 gttaagtcag gaagaaaact caaatgctaa tcataatacg ctataagagg aagctcttgt
      301 tgctatagcc ctattatcaa gacgttcggt agactgtaca cgacgtaaca aagaagataa
      361 tcctctttca gctaagatcc cctccagggg agtacgagga aaccaggttg aagaaagtgg
      421 agttatatgt ctctcattta attttctttc caaagcctta gtcacaccgc cagaaaacac
      481 gctaaatagc gttgagaaac atccattttc cggtgttatt ctttgtactt tgtacacata
      541 atcatctttt gggccatctt taataaagtg aacagcatag tctttgcctc taaaagagac
      601 aatggtggaa tcccctattt ctggatatac ccgatgatgg cgttgttgca ttcgggcttt
      661 aagctgaatt tcatctacga tatcttcatt gggtatatga agccttctgt tgaacctgtt
      721 ccatgacgca tgttcacttg tcagaaatgt cttgaatacc gctcctgata acgacatgat
      781 acatctccct atataacgta ttcctgatta atcacatact atgctaacag taaaataaaa
      841 atcttcgttg atttttacaa tactcccgta tgctatcgtc gtattattgc gtattaactg
      901 acaaccacca aaaagatggt atccatctga attaataatg tcatcttttt taacattacg
      961 caacatgtgt atatcaatat ggactgtacc gatcgcacaa taaatgcgat tcatgacagt
     1021 ctcattgcta agagcagata acagatccgc agccagaaca tccgtgacag ataaattatt
     1081 atcactcaga tttactgttg ctaaaggggt attaataaca atccagcacc ttctctcttg
     1141 actatcatca acatgataga gtctcagact atcaccaact ctcaactctg taactggata
     1201 atcagtccac ccaggtacga tatagccatc aaaaaaaaga gcattagaat gtggaactaa
     1261 ttgccaatta tcattaatca gatattgtaa actttgtacc gttgtttcga gtataattac
     1321 attaagtctg ttttctccat tattattttc tattaccgga taaatatctt tatttagcat
     1381 cgtaaattga acatcatcaa ctgacacatc accttccggt aaggcagtct gcattaccca
     1441 taagatcatg agttttatcg tatcttccgg tatcagccgg ctttcgctat acgggaataa
     1501 ctcttggcac cactgaatga actgttcttc tgatatccag aaacgcccct catagcccga
     1561 gtatgtcaac gtgaaacgaa taccatgacc gctgacctca cgaaaatatg catggcaagc
     1621 atgacctttg gacgtaatat agaggcgacc ggttccgata actggcaaca acggcaaata
     1681 aaaatcgtta attttcatat tcaattgtga actcaatggc ttcatcagga aagatttctt
     1741 ttaacttatc gaaaacatag tct
//
LOCUS       DABSYT020000291         1763 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.293, whole genome shotgun sequence.
ACCESSION   DABSYT020000291 DABSYT020000000
VERSION     DABSYT020000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.293"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..1607
                     /locus_tag="ID725_RS26160"
                     /old_locus_tag="pgaptmp_005232"
     CDS             <1..1607
                     /locus_tag="ID725_RS26160"
                     /old_locus_tag="pgaptmp_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGV7853605.1"
                     /translation="TVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVT
                     TAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYF
                     QITATPHLAVYDPTVQFEFWFSEKRIADIRQVETAARYLGSALYWIAASINIKPGHDY
                     YFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPD
                     LAEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDG
                     RLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLK
                     YLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLS
                     ANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHE
                     KEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVN
                     NWYPTASISDLLVVVMKKATAGITIS"
     gene            1675..>1763
                     /locus_tag="ID725_RS26165"
                     /old_locus_tag="pgaptmp_005233"
     CDS             1675..>1763
                     /locus_tag="ID725_RS26165"
                     /old_locus_tag="pgaptmp_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGV7853606.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQST"
BASE COUNT          444 a          423 c          507 g          389 t
ORIGIN      
        1 tgacggtgga ggtgagtgca gctgacggcc agtatctggc gcaggctaaa tgggacacgc
       61 cgcgggtggt gaagggcgtg cgcttcagtc tgcgcctgac cagtggtaag ggaacggatg
      121 ccagactggt gaccaccgcc atcaccgcag acacggagca ccgtttcagc ggcctgccgc
      181 tcggggaata caccctgacg gtgcgggcga taaacagcta tggccagcag ggtgaacctg
      241 ccaccaccac cttccggatt gccgcaccgg cagcaccgtc gcggattgag ctgacgccgg
      301 gctattttca gataaccgcc acgccgcatc ttgccgttta tgacccgacg gtacagtttg
      361 agttctggtt ctcggaaaag cggattgcgg atatcaggca ggttgaaacc gcagcccgct
      421 atcttggctc ggcgctgtac tggatagctg ccagtatcaa tatcaaaccg ggccatgatt
      481 attattttta tatccgcagt gtgaatactg ttggcaaatc ggcattcgtg gaggctgtcg
      541 gtcgggcgag cgatgatgcg gaaggttacc tggatttttt caaaggagaa atcgggaaaa
      601 cacatctggc ccaggagctg tggacgcaga ttgataacgg tcagcttgcg ccggacctgg
      661 ctgaaatcag gacgtccatt acgaatgtca gcaatgaaat cacgcagacc gtcaataaaa
      721 aactggaaaa tcagagtgcg gcaatccagc agatacagaa agttcaggtt gatacaaata
      781 ataacctgaa cagcatgtgg gccgtgaaac tgcagcagat gcaggacgga cgcctttata
      841 ttgcgggtat cggtgccggt attgagaata cgccagcagg aatgcagagt caggtgctgc
      901 tggcggcaga caggattgcg atgattaatc ctgcgaatgg caacacaaag ccgatgtttg
      961 ttggtcaggg cgatcagata tttatgaatg aagtgttcct gaaatatctg acggctccca
     1021 ccattaccag cggcggtaat cctccggcat tttccctgac accggacggg cggctgacgg
     1081 cgaaaaatgc cgatatcagc ggtaacgtga atgcgaactc cgggacgctc aacaacgtca
     1141 cgattaacga gaactgtcgg gttctgggaa aattgtccgc gaaccagatt gaaggcgatc
     1201 tcgttaaaac agtgggcaaa gctttccccc gggactcccg tgcaccggag cggtggccat
     1261 caggaaccat taccgtcagg gtttatgacg atcagccgtt tgaccggcag attgttattc
     1321 cggcggtggc attcagcggc gctaaacatg agaaagagca tactgatatt tactcctcat
     1381 gccgtctgat agtgcggaaa aacggtgctg aaatttataa ccgtaccgcg ctggataata
     1441 cgctgattta cagtggtgtt attgatatgc ctgccggtca cggtcacatg acactggagt
     1501 tttcggtgtc agcatggctg gtaaataact ggtatcccac agcaagtatc agcgatttgc
     1561 tggttgtggt gatgaagaaa gccactgcag gcatcacgat tagctgaatt ttataaccca
     1621 gatacgggcg ccagaaatgg cgcctttttt attgcagaaa agcgagaggt aattatgcgt
     1681 aaattatgtg ctgttatttt gtccgcagta gtctggcagg tcgccgctgc tacgccagcg
     1741 agtgcagcag aacatcagtc cac
//
LOCUS       DABSYT020000292         1749 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.294, whole genome shotgun sequence.
ACCESSION   DABSYT020000292 DABSYT020000000
VERSION     DABSYT020000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.294"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..145
                     /locus_tag="ID725_RS26170"
                     /old_locus_tag="pgaptmp_005234"
     CDS             <1..145
                     /locus_tag="ID725_RS26170"
                     /old_locus_tag="pgaptmp_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HGV7853607.1"
                     /translation="LYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIAL
                     PAE"
     gene            463..770
                     /locus_tag="ID725_RS26175"
                     /old_locus_tag="pgaptmp_005235"
                     /pseudo
     CDS             463..770
                     /locus_tag="ID725_RS26175"
                     /old_locus_tag="pgaptmp_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1010..1615)
                     /gene="espF"
                     /locus_tag="ID725_RS26180"
                     /old_locus_tag="pgaptmp_005236"
     CDS             complement(1010..1615)
                     /gene="espF"
                     /locus_tag="ID725_RS26180"
                     /old_locus_tag="pgaptmp_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HGV7853608.1"
                     /translation="MLNGISNAASTLGRQLVGIASRVSSAGGTGFSVAPQAVRLTPVK
                     VHSPFSPGSSNVNARTIFNVSSQVTSFTPSRPAPPPPTSGQASGASRPLPPIAQALKE
                     HLAAYEKSKGPEALGFKPARQAPPPPTSGQASGASRPLPPIAQALKEHLAAYEKSKGP
                     EALGFKPARQAPPPPTGPSGLPPLAQALKDHLAAYEQSKKG"
BASE COUNT          496 a          423 c          381 g          449 t
ORIGIN      
        1 actgtacagc ctgatcggga cgtgcaaact gaatgacgtg gatccagaaa gctaccttcg
       61 ccatgtgctt ggcgtcatag cagactggcc ggtcaaccgg gtcagcgaac tgcttccgtg
      121 gcgcatagca ctgccagctg aataacacat ccccgtcaat acggccctcg ctgtacgctt
      181 accaacaaaa ccccttttaa tcccttatat atcaggcttt ctttgtgtac aggggtcaac
      241 cgactcaacc tgtatcaagt tgcttttgag gatactttag ggggtattat ttcctcttga
      301 aatattagca ccccataatg ggaaaaaggt aatacataac aatggttttt aacagtatct
      361 ttgttataca agggggcatt gaggatatca ggaaaaatct caaaatcggt acggacagga
      421 aactacgtgg gcagcaaaaa cgtatggtaa caccgggaaa tgcaacgccc accagtcggg
      481 ctaatccacc actccaatcg tggttcgcag tactgcacac acgattaccg ggctatacag
      541 gagcggtttg gtctgaaaac atccatgccg cgtaaaggta accgttacga caacacctca
      601 atgaaaaact tatagtaaac gctgaagaat gaaactatcg ttttaataac cgggatgaag
      661 ccatctaagt aatacggaaa tacattgaga ttttctacaa ccatcagcgc cgccactccc
      721 gtctggggaa tatctccctg gtagccttca ggaaaaatat aaccagataa ctgcgtaaaa
      781 aaagaacaaa tggtggtgtc cgctattgcc agtatacctg agtttactgc ctttcagatt
      841 atcacaatca atagcgaaca gacttttaag ctctgtgcgc ttttaacaaa acgtcccttt
      901 ctccactttc accgtataat aatacattat ttattacccc tccaataaaa accgatatta
      961 ctaatcacat ttattaccac acaatttgat atcggttttt attagttggt taccctttct
     1021 tcgattgctc ataggcagct aaatgatctt ttaatgcctg tgcaaggggc ggtagtccac
     1081 taggccctgt cggtggcggt ggtgcctgac gggcgggctt aaaacctaaa gcctcaggac
     1141 ctttcgattt ttcataggca gccaagtgct cttttaatgc ctgtgcaatg ggcggtaaag
     1201 gtcgggatgc cccggatgcc tgtccacttg tcggtggcgg cggtgcctga cgggcgggct
     1261 taaaacctaa agcctcagga cctttcgatt tttcataggc agccaagtgc tcttttaatg
     1321 cctgtgcaat gggcggtaaa ggtcgggatg ccccggatgc ctgtccactt gtcggtggcg
     1381 gcggtgccgg acgagaggga gtaaatgaag tcacctggct gctcacatta aaaatcgttc
     1441 tcgcattaac attcgacgag cctggagaaa aaggggaatg aactttcacc ggagtaagac
     1501 gcacggcctg aggggctaca gaaaatccag ttccccccgc agagctcact cgacttgcga
     1561 tacctacaag ctgccgccct agtgtagaag cagcgttact aattccatta agcataattt
     1621 atgcctcttt atatctaagc ttggctaact ctcatatata tcaaaacgaa taataaaatg
     1681 atttttaaat caatataaat taaaatcctc gtacccagcc actaccattc tcatggacca
     1741 aactgtaca
//
LOCUS       DABSYT020000293         1745 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.295, whole genome shotgun sequence.
ACCESSION   DABSYT020000293 DABSYT020000000
VERSION     DABSYT020000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.295"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            72..224
                     /locus_tag="ID725_RS26185"
                     /old_locus_tag="pgaptmp_005237"
     CDS             72..224
                     /locus_tag="ID725_RS26185"
                     /old_locus_tag="pgaptmp_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGV7853609.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            705..893
                     /gene="dicB"
                     /locus_tag="ID725_RS26190"
                     /old_locus_tag="pgaptmp_005238"
     CDS             705..893
                     /gene="dicB"
                     /locus_tag="ID725_RS26190"
                     /old_locus_tag="pgaptmp_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGV7853610.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            890..1078
                     /locus_tag="ID725_RS26195"
                     /old_locus_tag="pgaptmp_005239"
     CDS             890..1078
                     /locus_tag="ID725_RS26195"
                     /old_locus_tag="pgaptmp_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGV7853611.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            1174..>1745
                     /locus_tag="ID725_RS26200"
                     /old_locus_tag="pgaptmp_005240"
     CDS             1174..>1745
                     /locus_tag="ID725_RS26200"
                     /old_locus_tag="pgaptmp_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGV7853612.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQA"
BASE COUNT          495 a          392 c          419 g          439 t
ORIGIN      
        1 ggaacacttt gtataacagc gcagcaggtt tttagttccg cgacccggcg ttaagggtaa
       61 atgaggtcaa catggatatg ctcaatcttg gcaacaatga atctctggtg tgcggagtat
      121 tccccaacca cgacggcacg tttaccgcga tgacgtatac cagaagtaaa acgtttaaaa
      181 ccgaagctgg cgcgcatcgc tggttagcaa gaaacgctaa ctgattagcg ccagtaaaaa
      241 caggtttcca caggttaatt taccctgaaa agtcagggca taacacgaaa gcgcacggcg
      301 aagttagtct ctctgtatag gtcgtcgtta aatttaattc gaccgtgcgc ttctggttgt
      361 ggcaatccgc gaaatggcgc ggcggtaagt atggcggggt tattccttcc cccgttgagg
      421 acaccgggtt gtcaggttga ccatacgctt aagtgacacc ccagccacaa caacctctgt
      481 tatcactttt ctggtgattc ggcggaaatg gatatccgcc ctttttaaag tgaattttgt
      541 gatgcggtga atgcggctat gcgcacgcgg aacagttaaa gcagtaaggc ggtcttttac
      601 tggcgtaacg agcatcaact aacccgacgt taattgttaa ctggttaacg tcacctggag
      661 gcaccaggca ctgcatcaca aaattcattg ttgaggacgc gataatggaa acgttattac
      721 caaacgttaa tacgtctgaa ggttgttttg atattggtgt tctgctcagt aataaagcgt
      781 ttactgagga tgccattaat atgcggaaat atgagcctta tctgctcaat gataattcca
      841 ttctttcccg aattgccctt attaaacttg gtattttcgg agaacgtcaa tgacttcagc
      901 atttgcactg gtgatgacgg tttttcttat aacgggtgaa tcacagaatg ttattaccgg
      961 aatttatgcc agtaaagaat cctgcctcca ggcaagagac gagcaaaaaa tttctggtga
     1021 atgcctaccg gtaaaaaaag tatcgctgta cctgaataac gaaacaccgg ctggataacc
     1081 cgccagccat attaacgcca taccaacgga ttaaaaatgc cagcaatggc agggattcgt
     1141 tcaccctgaa atctgtaatg aggttaaaac acaatgagta aaatctttat ttgcgctgct
     1201 attcctgacg aacaggccat aaaagaagat agcgctgttg cggtggccac tgccattgaa
     1261 gccggtgatg agcgtcgcgc acgcgcaaaa tttcactggc aatttctgga gcaattcccg
     1321 gcagctcagg actgcgctta taaatttatt gtctgcgagg ataaacccgg cataccccgc
     1381 cctgccctcg attcctggga taccgaatat atgcaggaaa accgctggga tgaggagtca
     1441 gcttcctttg taccggtcga accagaatcc gatccgatga acgtcaattt tgacaagctg
     1501 tcccctgaag tacagaacgc agtcctggtt aaattcgaca catgcgaaaa catcaccgtt
     1561 gatatggtga ttagcgcaca ggaattacta caggaagaca tggcaacatt cggcggacat
     1621 atcgttgaag cgttgatgaa aatgccagaa gttaacgcca tgtatcctga acttaaactg
     1681 catgccatcg ggtgggttaa gcataaatgt gagcctggcg ctaaatggcc tgaaattcag
     1741 gcaga
//
LOCUS       DABSYT020000294         1741 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.296, whole genome shotgun sequence.
ACCESSION   DABSYT020000294 DABSYT020000000
VERSION     DABSYT020000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.296"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(475..>1741)
                     /gene="nleL"
                     /locus_tag="ID725_RS26205"
                     /old_locus_tag="pgaptmp_005241"
     CDS             complement(475..>1741)
                     /gene="nleL"
                     /locus_tag="ID725_RS26205"
                     /old_locus_tag="pgaptmp_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HGV7853613.1"
                     /translation="KSILLTMNTSDDKYNHDKVRAAEELIKKISLDELAAFRPYVKMS
                     LADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERH
                     PGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGK
                     PDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNT
                     FGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEALNSNKSDYK
                     MIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGGVFCK
                     YSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVFTC
                     TAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          630 a          368 c          231 g          512 t
ORIGIN      
        1 gaatatcaat tcataaaata tgattctcca cgatttgccc agaaataacg aacataattt
       61 tcgttgtaat taaaatcata tttcatctat agtgaccatt cacagcaaaa caaaatcaat
      121 tatcacattc aaaaaaatac tgacaacaaa gatactcttc agacaaagtt gtcatatgta
      181 aattttccct taaaacgttg ctcaagaaaa atataaaaat catatgccct aatcaacatc
      241 ctcaagcact acgatatatg cagtatccta ttcatatatt accaccatat cagacaaatc
      301 gaatcacaac gagttctatc gtactctttc atcttcgaca ctgtaaattt taagaacatt
      361 cggctcatgc cactaaacca agacataata acaacggagc aataaatgtt cccactgaac
      421 atgtgcaaac ctaaaaagac agtgttacaa taatattcat ccatggtctc taaatcaacg
      481 ccacgcaaca ggataataca atgaaaatat ttcaggaaaa gattcttttg catgctccgt
      541 tagcatatca gtcagcacag cggtacaagt aaaaacatta tttcttcctt gtagcctgtc
      601 taaaatatca ttgtaataat aaactgagcc aaatacctga ggatctgttt tataagcttg
      661 ttcaatcaat ccatttgcat atctccgtag aatctcagga gaatcatact ctgtaccaaa
      721 catatcactc gatgagtatt tacagaaaac cccccctaaa caaaataata tttcagcctg
      781 ttttcttagt ggcatatctt tcaatacatg gctccccata atcatatcta catgctgagc
      841 gttcagttcc caaccatcaa gaaagggatt ccagacacag gcaagtttcc tttgctgatc
      901 atcagcaatc attttataat ctgatttatt ggaattaagt gcctcaataa atctctcttt
      961 gagttcatta tcagaaatga gtatatccag aataccagaa acgaaattct gggaaaaagc
     1021 tttatgataa ggaatactga aaattggaaa tgattcagaa aaaagattgc tcaatgaata
     1081 cccaaatgta ttttgaactt ctccatcctt atataaaaaa aaggacatcc aattagttga
     1141 tgtattagga gataacatat caaccatatg actcaaagaa cacatcattg ccataccatc
     1201 atcctgtgat gataacaaaa cccagttata agccaaatcg gaatcatcat cccagtcagg
     1261 cttcccagag ccatcgcaca atccaaaatc actctgctgt gtgacaggat aaactttttc
     1321 attttttaaa tatacttcat agagttccct aaatcgttcc tgcattccat cacgccgtga
     1381 accattcatt acaatttgta taaaggaact attataagat accatcgcac ctggatgtcg
     1441 ctcaaaatac atccctgcct gcaataaact accattctcc atatacatca ttattgaatt
     1501 attaaaccaa gacattatag tatcaaaaaa gtcatcacat ataggctcga gccattgctg
     1561 tatatttgcg ttgttcaaat aaggatgaat actaaatgaa tcagccaaag acatcttaac
     1621 atagggccgg aacgccgcta attcgtcaag agatattttt ttgataagtt cttcagcggc
     1681 ccttacttta tcatggttat acttatcatc agatgtattc atagtcaata aaattgattt
     1741 a
//
LOCUS       DABSYT020000295         1727 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.297, whole genome shotgun sequence.
ACCESSION   DABSYT020000295 DABSYT020000000
VERSION     DABSYT020000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.297"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..376)
                     /locus_tag="ID725_RS26210"
                     /old_locus_tag="pgaptmp_005242"
     CDS             complement(<1..376)
                     /locus_tag="ID725_RS26210"
                     /old_locus_tag="pgaptmp_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGV7853614.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATRAPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPEPRIKQLARRSTVTPGGAAC"
     gene            620..943
                     /locus_tag="ID725_RS26215"
                     /old_locus_tag="pgaptmp_005243"
     CDS             620..943
                     /locus_tag="ID725_RS26215"
                     /old_locus_tag="pgaptmp_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGV7853615.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            complement(1240..1509)
                     /locus_tag="ID725_RS26220"
                     /old_locus_tag="pgaptmp_005244"
     CDS             complement(1240..1509)
                     /locus_tag="ID725_RS26220"
                     /old_locus_tag="pgaptmp_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGV7853616.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1521..>1727)
                     /locus_tag="ID725_RS26225"
                     /old_locus_tag="pgaptmp_005245"
     CDS             complement(1521..>1727)
                     /locus_tag="ID725_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin subunit A"
                     /protein_id="HGV7853617.1"
                     /translation="AILGTVAVILNCHHQGARSVRAVNEDSQPECQITGDRPVIKINN
                     TLWESNTAAAFLNRKSQFLYTTGK"
BASE COUNT          494 a          456 c          331 g          446 t
ORIGIN      
        1 tgcatgctgc accacccggt gtcaccgtac tgcgacgcgc cagttgctta atacgcggct
       61 ccggacggtc atatgtctcc ggcagcggaa gaccttcacc atacgacatg ccgtttgact
      121 gccctgccag aaccacaaca aagtaatact ccggggctct ggtggcactg acagccaccg
      181 ccttaccctc attaccggtc accgccactg gtgtggtgac atcaccttcc gccgcaatgg
      241 cctgcatcag ggtgtaaggc gtgatggcca ccggactgcc aaatggctgc cagccctcct
      301 tcagtttttg tgtcagtcgt tcagcaaggt ctgacggcga tgccgccctg accacatcgt
      361 agtgtttaaa tgtcataacc tctcctgccg ccagcaaagt aattatccgg aacacacaat
      421 catgtaacac ataaaaaaca cgctataaaa aaaccatgtc tcttatataa caaaaaaacg
      481 ccgacaattt aaaatgccct cttattttaa acaaagaata aacgacacca gaaaaacaac
      541 tcagcctgac agattgcttt caaaacatca cctataattt atggcatcat ttaaaacctt
      601 aagaaaataa ggaaagcaaa tgaactggat agtgattgac acggtaatcc agccagcatg
      661 tggcatatca ttttcagcca tatggtgtaa cataaaatta atattatggt atcagtcaga
      721 taccttcctc cctccgggct gtatttttac accaacccgg acaggcgtaa tactcaataa
      781 taaagagctc cccgtcacca tttacaacgt ggccccattt aataaaaacg tctggaattt
      841 aattaaaaac agccaggagt gccctgcaaa cacaggaaat atcacagata agtgttttaa
      901 tcatcgctgc attctggaaa tatgcccata cgggcgaaaa taacagaatc attcagataa
      961 aaagcccctc cggagagggg ctgacactgc gtatctgtat catcatgtac atggtgccgg
     1021 gtgcctcccg gtgagttcag tccggtgtcc ctgaacccgc gtatctcgct ccaggttgtc
     1081 gtcagagatg acaccttata caccagtcgc ccctccgcac agggggattc accatgcgaa
     1141 tttttttaac gaatgctcag tctgacaggc aactgtcaac tgactgaatt gtgacacaga
     1201 ttacacttgt tacccacata ccacgaatca ggttatgcct cagtcattat taaactgcac
     1261 ttcagcaaat ccggagcctg attcacaggt actggatttg attgtgacag tcattcctgt
     1321 caactgagca ctttgcagta acggttgcag attccagcga ctggtccagt actcttttcc
     1381 ggccactttt actgtgaatg tatcattctc attatacttg gaaaactcaa ttttaccttt
     1441 agcgcaatcc gccgccattg cattaacaga aactaatgca aataaaaccg ccataaacat
     1501 cttcttcata cttaactcct ttatttaccc gttgtatata aaaactgtga ctttctgttc
     1561 agaaacgctg cagctgtatt actttcccat aatgtattgt ttatttttat aacgggcctg
     1621 tcgccagtta tctgacattc tggttgacta tcttcattca cggcgcgaac agaacgcgcc
     1681 ccctgatgat gacaattcag tataacggcc acagtgccca gtatcgc
//
LOCUS       DABSYT020000296         1704 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.298, whole genome shotgun sequence.
ACCESSION   DABSYT020000296 DABSYT020000000
VERSION     DABSYT020000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.298"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..400)
                     /locus_tag="ID725_RS26230"
                     /old_locus_tag="pgaptmp_005246"
     CDS             complement(<1..400)
                     /locus_tag="ID725_RS26230"
                     /old_locus_tag="pgaptmp_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HGV7853618.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMDRRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTGNKILMEKINSCVFGTDSNHFSCP"
     gene            complement(473..1045)
                     /locus_tag="ID725_RS26235"
                     /old_locus_tag="pgaptmp_005247"
     CDS             complement(473..1045)
                     /locus_tag="ID725_RS26235"
                     /old_locus_tag="pgaptmp_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGV7853619.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGQGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPEKGVFVKNSDGSDVCTLFDAAAFSRLTGEDLPHPL
                     TREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            complement(1078..1155)
                     /locus_tag="ID725_RS26240"
                     /old_locus_tag="pgaptmp_005248"
                     /pseudo
     CDS             complement(1078..1155)
                     /locus_tag="ID725_RS26240"
                     /old_locus_tag="pgaptmp_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(1157..1426)
                     /locus_tag="ID725_RS26245"
                     /old_locus_tag="pgaptmp_005249"
     CDS             complement(1157..1426)
                     /locus_tag="ID725_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853620.1"
                     /translation="MNILKKIMQRLCGCGKHDDCEHGQSLTVQLRLGPADILESDENG
                     IIPEQVRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(1428..>1704)
                     /locus_tag="ID725_RS26250"
                     /old_locus_tag="pgaptmp_005250"
     CDS             complement(1428..>1704)
                     /locus_tag="ID725_RS26250"
                     /old_locus_tag="pgaptmp_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853621.1"
                     /translation="GATGPQGPKGDPGETQIRFRLGPGNIIETNSNGWFPDTDGALIT
                     GLTFLAPKDATRVQVFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          456 a          467 c          299 g          482 t
ORIGIN      
        1 ccgggcaaga aaagtgatta gaatccgttc caaatacaca agaatttatt ttttccatta
       61 atattttatt acctgtttta ttttctctgt gttctgccat gacactctga aaatagttat
      121 taacactact taaaaaatcg acacctccat ttaattgcct ttccagagcc acagcccttc
      181 tatccattcg atgctcacgc cccaatagcc tatccagtaa gcctcctctc accggttcaa
      241 ctgaaaatcc atccagtagt tggacatagt gaacagaata ttggccacta ccaatggata
      301 ttcttattcc actttcaagt ctggcagcat cctgcaaaga ctgtactcct gcaggagata
      361 attgagatat acttgggata cttaaggtgg taacaggcat aaaaacctca ttcagtaatt
      421 catatcagtt tgcaggatgt ttcattaaat aaaataaagg gtaatgttca tttcaattac
      481 cctttataac gaagtttcct ctggtatcgt cataaatgca ttcttcatgt tttacaatta
      541 ttgatgccgt tattggttcc cgggtcagtg ggtggggtaa gtcttcacca gtcaaacgag
      601 aaaatgcagc ggcatcaaat aaagtacata catctgaacc atctgaattc ttcacaaaaa
      661 cacctttttc aggctgctcc agtgtaattg gacattgaag cgcatcacca ggacactgaa
      721 gtttttcaat atcgacagta aaggcacact ccgatatttt ttcctgcaaa gagtttcgcc
      781 ctcccattcc agggagcgca cttaatatcc tgtttctgag cacattaact ccatcattaa
      841 aagcaatatt caataattct gcataaaccc gtccctgccc ttcactttct gctaaaaaac
      901 ggcctgtatc tggcatataa gaaactctaa ccctctcccc tcccaggaga aatccaccaa
      961 tctgcccctc cattacctgc tgacgcagct ccatcatctc attcacgaat gaagccgatg
     1021 acacatatga tattaatcca ggcattacca tgctccttca tacggataaa tattccctca
     1081 agtcttcatt aacaaacatc caccggacat gacaacaaaa accggagccg gactccggtt
     1141 ttgtgaagct gtcgggttac ttcatcccgc caatattttc ccacgtcccg tcagcacgca
     1201 ggatttgcag cggtcttacc acacactgta tctgcttttt atccgcatcc agtatcacca
     1261 cctgcgtgat taccctgacc tgctccggga taatgccatt ctcatctgac tccaggatgt
     1321 ctgccggccc cagtcgcagt tgtactgtaa gcgactgccc gtgttcacag tcatcatgct
     1381 ttccgcaacc gcacagacgc tgcataattt tttttaatat gttcatgtca ttctcctgtt
     1441 ctgcctgtat cactgcccac ttcatccagc cccttaacat cctgccacgg cccgtcacca
     1501 aacctgacct gcaaatgctg aaaaaaaacc tgaacccgtg tggcatcttt gggggcaaga
     1561 aaggtcagtc cggtgatgag tgcgccatct gtatccggga accagccatt gctgtttgtc
     1621 tcaataatgt ttcccggccc cagacgaaaa cggatttgtg tctcccccgg gtcgcccttc
     1681 ggtccctgag gtccggttgc cccc
//
LOCUS       DABSYT020000297         1693 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.299, whole genome shotgun sequence.
ACCESSION   DABSYT020000297 DABSYT020000000
VERSION     DABSYT020000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.299"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>1693
                     /locus_tag="ID725_RS26255"
                     /old_locus_tag="pgaptmp_005251"
     CDS             <1..>1693
                     /locus_tag="ID725_RS26255"
                     /old_locus_tag="pgaptmp_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGV7853622.1"
                     /translation="REVTLPETGAATVNLINGSGKPVSVDITAHPAPDRIQVSTLPDG
                     VETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSG
                     TLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGVRFSLRLTSGSGEGSRLVTT
                     AITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAVPATIELTPGYFQ
                     ITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWTVQGSRIKPGTDFW
                     FYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEM
                     AEMKTTITETRNEITQTVSKTLENQSAIIQQIQRVQKDTNDDLAALYMLKVQKTKNGI
                     PYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKR
                     LFAVSITSSANPPTFSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSA
                     NQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAK"
BASE COUNT          403 a          447 c          518 g          325 t
ORIGIN      
        1 accgtgaggt gacactgccg gagaccggtg ccgccacggt gaacctgatt aacggcagcg
       61 gtaagccggt gagtgtggac atcaccgcac accccgcgcc ggaccggata caggtcagca
      121 ccctgcctga tggtgtggag acatacggtg tatggggact atccctgccg tcactgcgcc
      181 gtcgcctgtt ccgctgtgtc tccgtccggg aaaacacgga cggcaccttt gccatcacgg
      241 cggtgcagca cgtaccggaa aaagaagcca tcgtggataa cggtgcccgc tttgagccgc
      301 agtcaggcac cctgaacagc gtcatccctc cggcagtgca gcacctgacg gtggaggtga
      361 gcgcagctga cggccagtat ctggcgcagg tgaaatggga cacgccgcgg gtggtgaagg
      421 gcgtgcgctt cagtctgcgc ctgaccagcg gaagcggaga aggcagccgt ctggtgacca
      481 ccgccatcac tgcggataca gagcatcgtt ccagtggtct gccgccgggg gaatacaccc
      541 tgacggtcag ggcgattaac agttatggcc agcaggggga accggccacc accacgttca
      601 ggattaatgc acctgcggta cccgccacga ttgagctgac accgggctat tttcagataa
      661 cagcggtccc gcgtcttgcg gtgtatgacc cgacggtaca gtttgagttc tggttttcgg
      721 agacaaaaat cgcagataca tctcaggtgg aaacctctgc ccgttatctg gggaccggca
      781 gtcagtggac tgtccaggga agccggatta agccggggac ggatttctgg ttttacgtgc
      841 gcagcgtcaa cctggtgggg aaatctgcgt ttgtggaagt cagcgggcag cccagcaatg
      901 atggtgaagg gtatctggaa tttttccggg aaaaaatagg aaaactgcat ctggctcagg
      961 ggctatggga gctgatagac aacagccagc ttgcggatga gatggcggag atgaagacca
     1021 ccatcacgga aacccgcaat gaaatcacac agacggtcag taaaacgctg gagaaccaga
     1081 gcgccatcat acagcagata cagcgcgtgc agaaggacac aaatgatgac cttgctgcac
     1141 tttacatgct gaaggtacag aaaacaaaaa atggcatacc ctatgttgcc ggtattggag
     1201 cggggattga ggatactgat ggccagcccc tgagcaacat actgctgctg gctgaccgta
     1261 ttgcgatgat taacccggag gacggcaaca ccacgccgtt atttgtggcg caggggaatc
     1321 agttgttcat gaacgatgtg ttcctgaaac gactgtttgc ggtgagtatc acgtcatccg
     1381 ccaatccccc gacgttttcc ctgacgccgg agggcaggct gaccgcaaga aatgctgata
     1441 tcagcggtaa cgtgaatgcg aattccggga cgctcaacaa cgtcacgatt aacgagaact
     1501 gtcgggttct gggaaaactg tccgcgaacc agattgaagg cgatctcgtt aaaacagtgg
     1561 gcaaagcttt cccccgggat tcccgtgcac cggagcggtg gccatcaggg accattaccg
     1621 tcagggttta tgacgatcag ccgtttgacc ggcagattgt tattccggcg gtggcattca
     1681 gcggcgctaa aca
//
LOCUS       DABSYT020000298         1690 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.300, whole genome shotgun sequence.
ACCESSION   DABSYT020000298 DABSYT020000000
VERSION     DABSYT020000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.300"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>1690
                     /locus_tag="ID725_RS26260"
                     /old_locus_tag="pgaptmp_005252"
     CDS             <1..>1690
                     /locus_tag="ID725_RS26260"
                     /old_locus_tag="pgaptmp_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGV7853623.1"
                     /translation="GAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
BASE COUNT          522 a          396 c          438 g          334 t
ORIGIN      
        1 ccggggctgt cactgttgcc attcgccgcg aatacaaaca gacatggaaa acactcgaca
       61 atgaactggc ctgcgccctc tggcccggtg atgtggatgc aggaaacatt gacggtacca
      121 tccatcgctg ggcgacaaat gaggttatcg acaaggatcg cgaagactgg aagcgtatct
      181 cagcatcaat gcgcaaacag cccgaagcac ttggctatga ccgtcagact atttttggcc
      241 ttgttcgcga acgtccgatc gatattcaca aagatcccgt tgcactgaac aaatatatca
      301 gtgaatacct gacgacaaag ggcgtgtttg aacatgaaga aacagaccag agctctactg
      361 atgctctcca gccgtcagca gcacaaactg ctccagtgga gacggcagaa tccgatactc
      421 aaaaaaatga aatcctggtg gaagctgaac catctgtaga gcgtgaagga ccattttatt
      481 tcgtctttac cgataagggc ggggaaaaat acggcagggc aaacaaactt tctggtctgg
      541 acaaggcgct ggctgccggc ggtaccgaaa tctcaaaaga agaatatttt gcccgaaaaa
      601 atggcacata cacgggctta ccgcaaaatg tggataccgc tgaagattcc gaacaatcag
      661 agccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc
      721 agcctgacgc cgataaattg cttgctgcat cacgtggtga atttgttgaa gggattagcg
      781 acccgaatga tccgaaatgg gtgaaaggga tccagagtcg cgacgctgag gaccagaatc
      841 agcccaacgt gaaacaaaat gagccagaag cggaacaaaa cagcccggat acgcaacaaa
      901 acgggccaga agaacaacaa ccagaaccag cagtgcaaca ggaactggaa aaagtttgca
      961 ccgcatgcgg tcagaccggt ggcggcaact gtcctgactg tggtgcggtg atggggaacg
     1021 caacctacct ggaaacattc gatgaagaga atcaggctga agctcagaaa aatgatccgg
     1081 aggaaatgga aggcactgaa catctgcaca aggagaacac tggcagcgat cagtatcacg
     1141 ccagcgataa taaaactggc gaaacagcaa atcccttaat taaagtgaac ggtcatcatg
     1201 aaatctcatc caccagcagg ttgtggcacc atctgatgat tgaccttgaa acaatgggaa
     1261 aaaatcctga tgcgccaata aactctatag ccggtaagtt ttttgatccg gcaaccggag
     1321 agatggggcc agaattcagc aaaactatcg atctggaaac cgcaggtggg gtcatcgatc
     1381 gggacaccat taagtggtgg ctgaaacagt cacgcgaagc acaatcctcc attctgaccg
     1441 atgaaatcac gttggatgat gcactgctgc aattccggga atttatcgac gaaaactccg
     1501 gtgaattttt tgttcaggtc tggggtaacg gtgcaacttt cgacaacgtg attttacgcc
     1561 gttcatatga acggcagggg atcccctgcc cgtggcgtta caccaatgat cgcgatgtaa
     1621 gaacgatggt tgctctggga ctggtgatgg atttcgacgc aagaacgact attccattcg
     1681 aaggtgaacg
//
LOCUS       DABSYT020000299         1683 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.301, whole genome shotgun sequence.
ACCESSION   DABSYT020000299 DABSYT020000000
VERSION     DABSYT020000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.301"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..907
                     /gene="espR4"
                     /locus_tag="ID725_RS26265"
                     /old_locus_tag="pgaptmp_005253"
     CDS             <1..907
                     /gene="espR4"
                     /locus_tag="ID725_RS26265"
                     /old_locus_tag="pgaptmp_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HGV7853624.1"
                     /translation="FRWGNYTLLFENYEAYDKTGSITIEKNQGEGTLPIRHKLEFIST
                     NIAELLDKLTKITDARLCKGFSDWASSVKEGGSNDLKENVDRALVRMFKCVKLHSNEL
                     DLSYLFLGSVPPLPDWIEMLSLVYNELDSIQVPESCKELELDFNNLTEFPQVPDGITL
                     ISVNNNLISHIDSFPPKAKKIFISHNKLSETPAIPDTAKVFDCGYNKIQEIRYFPKNL
                     KEARIGYNNIEVVPAIPGNLKILFMECNPIKEAFLMPWTLTGICYEISQRKYIVTNPA
                     DYDKYSDMVKKHVIDGEELIIKYFM"
     gene            complement(1045..1374)
                     /locus_tag="ID725_RS26270"
                     /old_locus_tag="pgaptmp_005254"
                     /pseudo
     CDS             complement(1045..1374)
                     /locus_tag="ID725_RS26270"
                     /old_locus_tag="pgaptmp_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1332..1542)
                     /locus_tag="ID725_RS26275"
                     /old_locus_tag="pgaptmp_005255"
                     /pseudo
     CDS             complement(1332..1542)
                     /locus_tag="ID725_RS26275"
                     /old_locus_tag="pgaptmp_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          514 a          320 c          352 g          497 t
ORIGIN      
        1 tttcaggtgg ggtaattata ctctattgtt tgaaaattat gaggcttatg ataagacggg
       61 gtctatcacg atagaaaaaa atcaggggga ggggacttta cccattcggc ataaattaga
      121 gtttatatcg actaatattg cagagttgct ggacaagtta acgaaaatta cagatgccag
      181 actttgcaaa ggcttcagtg actgggctag ttcagtcaaa gaaggcggat cgaatgacct
      241 caaagaaaat gtagatagag cgttggtgag aatgtttaaa tgtgttaagc ttcacagtaa
      301 tgaacttgac ttatcatacc tatttttggg ttctgtgccg cctcttccag attggattga
      361 aatgcttagc cttgtttata atgaacttga ttcaatacag gttcccgaat catgcaaaga
      421 attagaactc gatttcaata accttacaga atttccacaa gtacctgatg gaattaccct
      481 gatctccgta aataataacc tgatatcgca tattgactca tttccgccaa aggctaagaa
      541 aatttttatt agtcacaata agctatcgga aacaccagca ataccagaca ccgctaaggt
      601 ttttgattgt ggctataata agatacaaga aattcgatac ttccccaaaa atctgaaaga
      661 agcgcgtatt ggatataata atattgaggt tgttcctgcg atacctggca atttaaaaat
      721 actttttatg gaatgtaatc ctattaaaga ggcattttta atgccatgga ctctgacagg
      781 gatctgctat gaaatatcgc agcgaaaata tattgttacg aatccagccg attatgataa
      841 atattccgat atggttaaaa agcatgtaat agatggtgag gaactcataa ttaaatattt
      901 tatgtaatta attaggcgta attgtgtcac tggaatagag gtttcttgtg acgcatgtgg
      961 aaggagcgtc tatggacaag gcgtttcctg ctggtgataa ataaaggcgc agcacgtcga
     1021 atgcaagaat gcgctgcgtc tggctcatgg caattcatta tcttcgcata gcgtcaatga
     1081 caaaaatcga ctgtgtggat caaattgata atcagcaaac ggttcgcaat caacgaaacc
     1141 atgcttcaaa taaagttgat ggcaggccgt aaatccagcc tgtgtacccg tttctaagct
     1201 caggcgatga aggcatctgt catgggcgac ctgcaaaatg tggcgtaaaa tcagactggc
     1261 gacaccccga cgtaaataat ttggcgcggt acgcattgat ttcagttcgc catgtttatc
     1321 atccagcaac tttagcgcac ccattcctgc gagttgttcg ccttcccata ctgacctgtt
     1381 gacctggctt gtttaatgac atccgatctc accgcgtggg gttatggatg gggcaaactc
     1441 actcaatttc tggtttaaaa tggaaacctg attctgaaag ttttctgcca cccacgatcc
     1501 gtatacgcga taaaccctct gatcatcaga atgtctcatt tgacacatca ccacgtcgga
     1561 tgttgttacg accacagacg caacagtaac ctctggataa cttaatcttc gttcttattt
     1621 ttattaatat actgcgttat aaaaatgagc gatttttgat agtggcaatt ttattgtgtt
     1681 tta
//
LOCUS       DABSYT020000300         1678 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.302, whole genome shotgun sequence.
ACCESSION   DABSYT020000300 DABSYT020000000
VERSION     DABSYT020000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.302"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(23..844)
                     /locus_tag="ID725_RS26280"
                     /old_locus_tag="pgaptmp_005256"
     CDS             complement(23..844)
                     /locus_tag="ID725_RS26280"
                     /old_locus_tag="pgaptmp_005256"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGV7853625.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            complement(1446..1580)
                     /locus_tag="ID725_RS26285"
                     /old_locus_tag="pgaptmp_005257"
     CDS             complement(1446..1580)
                     /locus_tag="ID725_RS26285"
                     /old_locus_tag="pgaptmp_005257"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853626.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
BASE COUNT          491 a          404 c          325 g          458 t
ORIGIN      
        1 tcagtgaata aaattttcat cattaattat ctggtaatct tggcccctta tgaagagcca
       61 gtactgaaga aaaaagtggc gttgtataat aaaaaaggcg tcgtttcagc cagccggaat
      121 gttcccgctc tttggtgctg atacgcccta tgcgtaatga accattttca ggacagttca
      181 ttcttctttc ggttgtatat aaataagaga aaccaagctt accggcgaga ttaatataat
      241 cccgattata atatccttca ggccagcaca gatgggaact acagaacccc agcttttccg
      301 tcagacattg ttttcccacc agaatatctt ctttcatgag ccggcactgt tcagcacgtg
      361 atacagacaa cctgtcccag cgtttatgtg aatgggtgtg cacatgaaat tcaaccagtc
      421 cggaatcacg catttcccgg acttcagacc agcgaagcat gacctcatct gaacggttat
      481 cggcaatcaa ccgttcacag tcgcggtgag aatattcctg ctcctgcctg ctacgtacgt
      541 ttcccttacc aatcagcccc gtaatgagaa aaatatgtgc atgaaggtta tattccttca
      601 ggaccggcca tgctctgagc cagttatcaa gataaccatc atcgaatgtg agcatgacac
      661 tctttcgtgg aagcgttccc ccctgataaa aatattcaag ctcagcagat gttaccgtcc
      721 gccagttatt atctgcgagc cacttcatct gttcacaaaa tgtttcaggt gagagtgtca
      781 caagccccgg acaacgactg acatgatgat acatcagtac gggaagatgc cttgcattca
      841 acataaaaaa ataatcgtct gttaagcatt ctgaaaatat cctcgcgtaa acaacagtga
      901 ggatccatag atatcacaca tggtgatatt attgtgtaat cccggaatgg tccggagatt
      961 accgacaaca gggatttttt aatattttta tcagttaatc aaccagaact taaatttccc
     1021 ttaacgcata tgctcttttt aatcagattt tctgtttttc agaaaaaaca gaataccgca
     1081 atttcagtaa acacagcgag agatatccgc ctcagtaaaa aatcagaaga ttatcatcct
     1141 gatttattgc ataaaaccag tcgagtagcg gaattttctg catccggata ccactccccc
     1201 tgaaaaatac tcgcacaata tcgcgccgca tgcactgatg gaccgggcca tcgtgagaat
     1261 ctcttgcatc gtagatatta accacaccag tgccccccag aacaacagta atacctgtag
     1321 cagacctgcc atagaggtta aataaccatt catatccctc ttctacccat tcatcccgaa
     1381 gtttcagcca gcgacgaatg gactgtacct cctgtttcag ccgaggggct gagttgacaa
     1441 accgtttaga gccgccggat attcaggtta ccggcctgca aatcaatatc agacagtttt
     1501 atacctaata actctgatgc ttggacacca tgaagatacc ccataaggag caggcagtga
     1561 ttacgttccg ggtgtggcac tttgaagaca gcactgtgca acttatgaat ctcacccggg
     1621 ttcagatatt tttgttgtcc attacctgtc cttatttatt gaaagtcgat attagttt
//
LOCUS       DABSYT020000301         1662 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.303, whole genome shotgun sequence.
ACCESSION   DABSYT020000301 DABSYT020000000
VERSION     DABSYT020000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.303"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            83..430
                     /gene="tnpB"
                     /locus_tag="ID725_RS26290"
                     /old_locus_tag="pgaptmp_005258"
     CDS             83..430
                     /gene="tnpB"
                     /locus_tag="ID725_RS26290"
                     /old_locus_tag="pgaptmp_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGV7853627.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            480..>1662
                     /locus_tag="ID725_RS26295"
                     /old_locus_tag="pgaptmp_005259"
     CDS             480..>1662
                     /locus_tag="ID725_RS26295"
                     /old_locus_tag="pgaptmp_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HGV7853628.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WL"
BASE COUNT          364 a          467 c          504 g          327 t
ORIGIN      
        1 taacgttccg gcacgggacg ctccgcttca atggcaatgt cagcgaaaag ctcctgactc
       61 tgctgataca ggaactgaag cgatgatccc gttaccttcc gggaccaaaa tttggctggt
      121 tgccggtatc accgatatga gaaatggctt caacggcctg gctgcgaaag tacaaacggc
      181 gctgaaagac gatcccatgt ccggccatgt tttcattttc cggggccgca gcggcagtca
      241 ggttaaactg ctgtggtcca ccggtgacgg actgtgcctc ctgaccaaac ggctggagcg
      301 tgggcgcttc gcctggccgt cagcccgtga tggcaaagtg ttccttacgc aggcgcagct
      361 ggcgatgctg ctggaaggta tcgactggcg acagcctaag cggctgctga cctccctgac
      421 catgctgtaa atctctttat cctggttgtc acagaataag cccggtaaaa tacgggctta
      481 tgaacgacat ctcttctgac gacatcttcc tgctgaaaca gcgcctggcc gaacaggaag
      541 cgctgatcca cgccctgcag gaaaagctga gcaaccggga gcgcgaaata gaccatctgc
      601 aggcgcagct ggataaactc cgccggatga acttcggcag tcgttccgaa aaagtctccc
      661 gccgtatcgc acaaatggaa gccgatctga accggcttca gaaagagagc gatacgctga
      721 ctggtagggt gtatgacccg gcagtacagc gtccgttgcg tcagacccgc acccgtaagc
      781 cgttccctga atcactaccc cgtgacgaaa agcgactgtt gcctgcggcg ccgtgctgcc
      841 cgaactgcgg cggttcactg agctatctgg gcgaggatac cgccgaacag ctggagttga
      901 tgcgtagcgc cttccgggtt atccggacgg tacgggaaaa acatgcctgt actcagtgcg
      961 atgccatcgt gcaggcacct gcaccttcgc ggcccatcga gcggggtatc gccggaccgg
     1021 ggctgctggc ccgcgtgctg acctcgaagt atgcagagca caccccgctg tatcgccagt
     1081 cagaaatata cggccggcaa ggtgtggagc tgaggcgttc actgctgtcg ggctgggtgg
     1141 atgcatgctg ccggctgctg tctccgctgg aagaggcgct tcatggctat gtcatgactg
     1201 acggcaaact ccatgccgat gataccccgg tccaggtact gctgccgggt aataagaaga
     1261 cgaagaccgg gcggttgtgg gcgtatgttc gtgatgaccg caatgcaggg tcagcgttgg
     1321 cacctgcagt gtggttcgct tacagcccgg acagaaaagg catccatccg cagactcatc
     1381 ttgcctgctt cagcggtgtg ctgcaagcgg atgcgtacgc cgggttcaac gagctgtatc
     1441 gcaatggtgg gataacggaa gctgcctgct gggctcatgc ccgccgaaag atccacgatg
     1501 tgcacgtccg catcccgtca gcactgacgg aagaagccct ggagcagatc ggtcagttgt
     1561 acgccataga ggcggatata aggggaatgc cggcagagca gcggcttgct gaacgtcagc
     1621 gaaaaacgaa accgttgttg aaatccctgg aaagctggtt gc
//
LOCUS       DABSYT020000302         1661 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.304, whole genome shotgun sequence.
ACCESSION   DABSYT020000302 DABSYT020000000
VERSION     DABSYT020000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.304"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            85..207
                     /locus_tag="ID725_RS26300"
                     /old_locus_tag="pgaptmp_005260"
     CDS             85..207
                     /locus_tag="ID725_RS26300"
                     /old_locus_tag="pgaptmp_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGV7853629.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            222..911
                     /locus_tag="ID725_RS26305"
                     /old_locus_tag="pgaptmp_005261"
     CDS             222..911
                     /locus_tag="ID725_RS26305"
                     /old_locus_tag="pgaptmp_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGV7853630.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            1095..>1661
                     /locus_tag="ID725_RS26310"
                     /old_locus_tag="pgaptmp_005262"
     CDS             1095..>1661
                     /locus_tag="ID725_RS26310"
                     /old_locus_tag="pgaptmp_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853631.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQ"
BASE COUNT          440 a          287 c          411 g          523 t
ORIGIN      
        1 taagaactgt tacaaaaaaa gggggggcaa ttacaggtag ttatggatga tgagtgaaac
       61 agatattgga gaaccgggga atgaatgatg tctgagtctt atatatcaga actccttcgc
      121 tgtcgctggg ggctcctgtg cttatgtcgt ttccccgatt cggttttgaa cgattaccga
      181 atgttgaaga attatgccaa aatatagaaa ggatttactg catgaatacc caatatttac
      241 agtatgttcg tgagcaactt atggcagcta ctgctgactt gaacggagca acgaaaggcc
      301 agctcgaagc ctggcaggag catgcacaat ttgatactgg tacatacaaa cgaaagaagc
      361 cgcgcattct ggatgtggta actggcaaga tgattacgct ggataatacg ccgacttccg
      421 gtaagcagtc gtacgcaaaa ggttcatcca ttgctttggt cagcccggtt gaattctcaa
      481 cctcttcatg gcgccgcgcg gttttgtctc tcgatgaaca tcagaaagca tggttgcttt
      541 ggtgttacag cgaaagcgtt cgatgggggc atcaggtcac cataacgcaa tgggcatgga
      601 gcgagtttaa agatttgtta agtaacagaa aaattgcagg taagacactg gatcgcctga
      661 agacgttaat ctggctggct gcacaggatg tgaagagcga acttgcaggg cgtgaggcct
      721 atgaatacca gacactggca tcattggtgg gagtgacaac aaaaaactgg tccgagacat
      781 ttactgaacg ctgggttgca atgaagcaca tttttctaca gcttgatagt gatgctttat
      841 tgcttgtgac gagaacacgt tcaaaacaaa aggcagcatt tttacagcaa aatattgcaa
      901 aactggatta aaagccatat acttcatgca aatttggtat gttgtaaaaa atgtataaac
      961 ccgctgccga gtgggttttt ttatgccctg agttgtactt gtacggtaaa catgctggct
     1021 gctatgtaat agagtttttt tagcctgtaa cctcttgacg gcattgaatt gcttttgtta
     1081 tgagttgtaa gccaatgtta tcatcttgta ttggggtggt tatgaaggat ggtgcgctgc
     1141 tcaggagttc ttcacttttt attgcctaca tgggatgcct tggatggggg agtgcttatt
     1201 tctatggatg gggtacttct ttttactacg gcttcccatg gtggattgta ggtgcaggtg
     1261 ttgatgatgt tgccagaagt ttattttttg cagttatcgt cattgctata tttcttatcg
     1321 gttggggtat tggtgttgta ttctttttcg cagtgaaaag aaaacattct atgcaagagc
     1381 taaatgtatt tcgcctttat tttgctgtgg aattattgtt tgtgccggca attattgagt
     1441 tttctatatt gagacagaag attcaggtac ctcttttgct actgtcagca gcgattgcgc
     1501 tggcggttac aatttcgata agatcttatg ggcgattttt atcggtatca tgcttctatg
     1561 ataagccatt tataaaaaaa catttttttg agattgtgat gattgctttt gtggcatatt
     1621 tctggctttt ttcatttctg acaggatatt acaaaccaca g
//
LOCUS       DABSYT020000303         1625 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.305, whole genome shotgun sequence.
ACCESSION   DABSYT020000303 DABSYT020000000
VERSION     DABSYT020000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.305"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(212..1087)
                     /locus_tag="ID725_RS26315"
                     /old_locus_tag="pgaptmp_005263"
     CDS             complement(212..1087)
                     /locus_tag="ID725_RS26315"
                     /old_locus_tag="pgaptmp_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGV7853632.1"
                     /translation="MFCIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTLGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
     gene            complement(1228..1497)
                     /locus_tag="ID725_RS26320"
                     /old_locus_tag="pgaptmp_005264"
     CDS             complement(1228..1497)
                     /locus_tag="ID725_RS26320"
                     /old_locus_tag="pgaptmp_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853633.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(1499..>1625)
                     /locus_tag="ID725_RS26325"
                     /old_locus_tag="pgaptmp_005265"
     CDS             complement(1499..>1625)
                     /locus_tag="ID725_RS26325"
                     /old_locus_tag="pgaptmp_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853634.1"
                     /translation="PKDTTQVQGLFRHLQVRFGDGPWQDVKGLNEVGSDTGRTGE"
BASE COUNT          415 a          382 c          316 g          512 t
ORIGIN      
        1 acatactatt agtgattctc atgcatcgta agcggctcgc cagaaccgta ttgatattta
       61 ctgagcgctc agatcaactt tccatggcaa cagatcgcgt accggtttgc cggccagtcc
      121 tggatatgct cccgccgaca atattaatgg ccaggaaatg tttaaaacaa tgattctgtt
      181 tcctaaccat caagtgtata tgctggctca attactcaat atctatgggt tgagttctac
      241 ccaaagtggc tctgtagtcg cttcttctta cagtatgttc aggtgttgca ggcacagttg
      301 tggagcgtag atgcgttgtt ggtttaccat gctctggtac ataaaaaacg ccagtgccat
      361 ggtaacctga tatagaaacg ttaattccgc gacgttcaaa ctcggcatag acgtgttctg
      421 ctaaagacct cttttgacca aataatgccc tggccaacca gccgttattc atatttgcgg
      481 atttttctat ttcatcagga gagaagtctt tgttttttat tatgttggct gagttacagg
      541 atgttaatct gatatcatct atttcatgaa gattatgctg aacaatgcgg tcgacaatat
      601 ctgacggtga taaaaattca tctccgcact taagaagagg tagaccagca gaaccatgcc
      661 cagacagata aattttgttg tattttccta gtgttaaatc atctggcagg atggttttca
      721 tctctgtcgc tgtaatactt atagctgcag cagcaacaac atcgctatta cttgattgta
      781 gatggctttt atttgctcca ggatatgtaa actccatttt ccttttatca aaatcttgtt
      841 ttgtagcatc attcaggaat aataaatcat acggtttttt ccccgttgtg actcttgaaa
      901 cattttggcc aagaatatta agagtatagt cattaacgga tttcattcct aaaaaaacaa
      961 gtaatccttc tttctcttgc gctttttcga ttgttctccc tgctaaagtt attgggatgg
     1021 attgagattg ttttgtcgga actgatacgc tggcgctaat aggtagtagg gttcttatgc
     1081 aaaacataca acctctttct taattttcga acctgtttag gattctgtgt aaattcaaaa
     1141 taaacctgtc tgaacattcc taacaaacat ccaccggaca tgacaacaaa aaccggagcc
     1201 ggactccggt ttttgtgaag ctgtcggcta tttcattccg ccaatatttt cccacgtccc
     1261 gtcagcacgc agaatttgca gcggtcttac cacgcactgt atctgctttt tatccgcatc
     1321 cagtatcacc acctgcgtga ttaccctgtc ctgctccgga ataataccat tctcatcgga
     1381 ctccaggatg tctgccggcc ccagtcgcag ttgtgctgta agcgactgca cgtgttcacg
     1441 gccatcatgc tttccgcaac cacacagacg ctgcataagt ttttttagta tattcatgtc
     1501 attctcctgt tctgcctgta tcactgccca cttcattcag ccccttaaca tcctgccacg
     1561 gcccgtcacc aaacctgacc tgcaaatgcc gaaacagccc ctgaacctgt gtggtatctt
     1621 tgggg
//
LOCUS       DABSYT020000304         1622 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.306, whole genome shotgun sequence.
ACCESSION   DABSYT020000304 DABSYT020000000
VERSION     DABSYT020000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.306"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(417..1355)
                     /gene="lrhA"
                     /locus_tag="ID725_RS26330"
                     /old_locus_tag="pgaptmp_005266"
     CDS             complement(417..1355)
                     /gene="lrhA"
                     /locus_tag="ID725_RS26330"
                     /old_locus_tag="pgaptmp_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGV7853635.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT          443 a          382 c          358 g          439 t
ORIGIN      
        1 atttaatgcc ctttcacccc agatccttga cgactccagg ataattagat gttgttgaat
       61 cgtgtccgtt gtgaagcaat ggaaaaaata cgggtctatt ttgacaggaa tttgtgtcga
      121 ttcctccccc caaaagagag tattttcttg atctgtgaca cgcttttgtc attccataac
      181 aaaaacgcag caacaaattt acgtattttt taacatcatt gtagcaggtg atttttttca
      241 ggcgattatt tgtgcgtacg gggcgtgaat ctctggtgat tgaaaaatga acagttttgt
      301 acgttctgca cgatgtgaag aaggttcttt actaaaaaaa agccgctggg gtttaaaaca
      361 cccccagcgg ctcgtttttt acactattgt ctcaggaatt atctatcgtc cgtcgattac
      421 tcgatatccc tttcaatcaa caatgaatca tccccttccg gagcagatat tgggctgtat
      481 tgccacggat tatggtagct ctccatcgcc tgatagatca cctgggccag ctcattatta
      541 ctggacgggt catagcacag caaatattcg gtatcaggta gcggcggtaa gccatctact
      601 ccactcagca cacgcaagtc cggactcatc atctcgaccg gccttgccgt cacgccaaga
      661 ccggctttca ctgccgcacg aacggccgga agcgtcgagg cgacataagc cagtcgccag
      721 ggaatatctg ctttattaag cgtcgccagc accatatcgc gaaacggact tggatcatcc
      781 agcaatacaa gagggatcgg ctcacctttt tgcaatacgt attccgctgc gcagtaccag
      841 tgtgttggcg aggtacgcag gttcaatgcc ttaaacgctg aaggacgatg ggtggttacc
      901 atcaaatcca cttcctggga ttccagcatt tctgccatat aggcattacg tttaacgcgg
      961 acatccagcg ctaatttcgg ataaaccgaa ctcacgcgat ttaacaggaa aggtaagatc
     1021 gtatcggcag attcatctga agcaccgata gttaacacgc cctgaagatt actgaacatt
     1081 aatgatgagc aggcctcatc attaaaacgc aggattttcc tggcgtaacc aagaagttga
     1141 atgccatgtt cagttaacag tttgttgcga ccgtgacgag cgaacagttc tttcccaacg
     1201 agttgttcca gacgctgcat ttgctgactt acggcggact gagtacgaca cacagcggca
     1261 gctgcggcag caaaagtgtt cagatcggca acagcaacaa atgttctcag cagatcgagg
     1321 tcgaggttaa ttatcggacg atttgcactt atcatatatt atcacttact ggcggctcat
     1381 actgagctgg ttaatgctgt gcacacacaa acaagcaatt ccatttgtaa tgtgcctccc
     1441 tggcagtttc atccggaacc cggacgaaag taaaaatgca tatgagttgc actaaaaaag
     1501 cgactcacat tgttccgtta taatgcctga agtagatcac agaatatatc ttcagggatc
     1561 gcatatctat taagttactc actcttttct atttatgaca tgcgcgtgtt tgtataaatg
     1621 ta
//
LOCUS       DABSYT020000305         1604 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.307, whole genome shotgun sequence.
ACCESSION   DABSYT020000305 DABSYT020000000
VERSION     DABSYT020000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.307"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>1604
                     /locus_tag="ID725_RS26335"
                     /old_locus_tag="pgaptmp_005267"
     CDS             <1..>1604
                     /locus_tag="ID725_RS26335"
                     /old_locus_tag="pgaptmp_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HGV7853636.1"
                     /translation="GLIKKYKYLSLSEIHRTLTNSTSFADISIPLLQTICPSITTIIK
                     KTEYYGHQLTNAMTVASVVKPYDFSNLGAINSIDKSVSDVPALHTIVEQAKYNLLSWN
                     DFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSKGKCLGLSLLYLDTGGYGGGYQK
                     LRHNIDTASTLYQTKYNDNLKLSDRDDFFLRKTQRIITMSNELGNNRLKNAQLEVLEL
                     KDPILTEGILYQRRISSLLITTEYHSLALQQISSFWRVTDPNFGHCDFHSLAQALTFI
                     KNITSNRNFSSLYGSGIVKIYFSESLNNWKYIKLPLVQTGSLLRDIYLTTPEKLSTSG
                     GSLNIMGHLVPVSFIYDIGGVINGNRISESTDVKNKIRSLKINGDILQHYINTHYLSE
                     EQTQKIKDIVDFLGIQDNTIKVKLESDIKPISEIQQPLHSILSRQKEHVKNLLSGLLD
                     EFSNKLRKQGLSLKTNVLSVNNFKESKINSDTVEVTVTDLQGRLYRVDIDTRVIGLTF
                     KEGINSLSEALEHMNIDAIMSVIGLV"
BASE COUNT          581 a          246 c          276 g          501 t
ORIGIN      
        1 aaggtttaat aaaaaaatat aaatatttat cgctgagtga aatacataga actttaacaa
       61 atagtacttc atttgcagat atttctatac cattattgca gaccatatgt cccagcataa
      121 caacaataat aaaaaaaaca gaatattatg ggcatcagtt aacaaatgct atgactgttg
      181 cttcagtggt taaaccatat gactttagca atcttggtgc cataaatagt atagataaat
      241 ccgtttctga tgtgcctgca ttgcatacaa ttgttgaaca agcaaaatat aatttacttt
      301 catggaatga tttttataac actcatgcgt caatatggga tacaatagca agacagcata
      361 aaagtacaaa tatcgaattt cacccacaaa gtcttctttt tgatagagat tcgaaaggaa
      421 agtgccttgg tttatctctt ctgtatcttg atacaggggg gtacggaggc ggatatcaaa
      481 aactgaggca caatattgac actgccagca cattatatca aacaaaatac aatgacaatc
      541 taaaattatc tgaccgtgat gatttctttt taagaaaaac acagcgtatc attacaatgt
      601 ccaatgagtt aggtaataac aggttaaaaa atgcacagtt agaggttctg gaattgaaag
      661 accctatact aactgaaggc atattatacc agcgtcgtat atcatctctt ttaataacaa
      721 cagaatatca ttcacttgca ttacaacaaa tatcttcttt ttggagagta actgatccta
      781 attttggtca ttgtgacttt cattccttag cacaggcttt aacgtttata aaaaatataa
      841 cgagcaatcg taacttttca tctttatatg gaagtggcat tgttaaaatt tacttcagtg
      901 aatctcttaa taattggaag tatattaagc tcccccttgt tcagactggt tctttgttaa
      961 gagacatata tttaacaacc ccagaaaaat tatccacaag cgggggttca ttaaatatta
     1021 tggggcatct cgtgcctgtc agttttattt atgatatcgg aggggttatt aatggtaaca
     1081 gaataagtga aagtacagat gtgaaaaata aaattaggag cttaaaaata aatggggata
     1141 ttttacaaca ttatataaat acgcattatt tgtcagagga gcaaactcag aagataaaag
     1201 atattgtgga ttttttaggt attcaggata acacaataaa agttaagctt gaaagtgata
     1261 ttaaacctat aagtgaaatc caacagcccc ttcattccat tctttcacgg cagaaagaac
     1321 acgtaaagaa tttactatct ggattgcttg atgagttttc aaataagtta agaaagcaag
     1381 gtctttcact taaaacaaat gttctttcag ttaataactt caaagaatca aagataaata
     1441 gtgatacagt ggaggttaca gtaacagatc tgcaaggaag actttatcgt gttgatatag
     1501 atacaagagt tattggctta acttttaaag aggggataaa tagtttatcc gaagcactcg
     1561 aacacatgaa tatcgatgcg attatgtctg ttattggctt ggtt
//
LOCUS       DABSYT020000306         1574 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.308, whole genome shotgun sequence.
ACCESSION   DABSYT020000306 DABSYT020000000
VERSION     DABSYT020000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.308"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..>1574)
                     /locus_tag="ID725_RS26340"
                     /old_locus_tag="pgaptmp_005268"
     CDS             complement(<1..>1574)
                     /locus_tag="ID725_RS26340"
                     /old_locus_tag="pgaptmp_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGV7853637.1"
                     /translation="TADYLSCLINKKHTIIPVSSTPLYESIADSDWYFGNDTLELRNN
                     ESDDKKFATNYVLKDFPMFTTPGQWDFLLKLPYEFIITQSFLFEAPAKTVRKIDSQLN
                     KMGSTGDAAKSQQEELIAGKDFVTSGTNLFGSYHCVLTVFGDTAEAARKNGIKVSSEF
                     ITTGKGFRFSRATSAAPYVFFSHLPMNKTRPLSTRRTAANLACTFSLHNYSSGKKSGN
                     PIGDGSALMPLKTVSDGLYYFNTHYSDPNKNVTGQRIAGHAMILGATGTGKTTFEGSA
                     AGFLQRFDPYMFVIDYNRSTELFVRAYGGSYFALQEGVFTGLNPWQLAEDESSPIWKR
                     LLAFLKSLAKAFVRDNMGNPCSDQQAIEMDAAVESLMTMPVQLRRTSALLQIVNDSEL
                     RIRLAKWCKNGAYSWATDSPENTFNPMDYKKVGFDTTVILEKVNGETHPACEPLLAIL
                     FFYKTLMQRDGKLMLTIVEEFWMPANFPLTQDMIKSALKAGRLKNEMMWLTSQSPEDA
                     VNCAIFAALVQQTATK"
BASE COUNT          463 a          349 c          307 g          455 t
ORIGIN      
        1 atctttgtgg ctgtttgctg aacaagagcc gcaaaaattg cacagttaac cgcatcctct
       61 ggcgactgac tcgttagcca catcatttca tttttcagac gtccagcttt taacgcagat
      121 ttgatcatat cctgagtcag agggaagttc gcaggcatcc agaactcctc gacaatagta
      181 agcattagct ttccgtcacg ttgcataagc gttttataga aaaataaaat cgccagcaga
      241 ggttcgcacg ccggatgcgt ttcaccattt accttttcaa ggataacggt tgtgtcaaaa
      301 cctacctttt tataatccat cggattaaaa gtgttctctg gcgaatctgt tgcccaggaa
      361 tacgcaccat ttttacacca tttagcaagt ctgatacgca gttcactatc gttaactatc
      421 tggagcaatg ctgacgttcg gcgcaactgg acaggcatag tcataagact ctctactgcc
      481 gcatccattt ctattgcctg ttggtcagaa caggggttcc ccatgttgtc ccgaacaaat
      541 gcctttgcaa gcgatttcag aaacgctaac aaacgcttcc agattggtga tgattcatct
      601 tccgcaagct gccacggatt aagaccagta aaaacacctt cctgtagagc aaaataacta
      661 ccaccatacg cacgaacaaa taattctgta gagcggttat agtcaataac gaacatgtaa
      721 ggatcaaaac gctgtaaaaa accagccgcg gatccttcaa aagtcgtttt accagtacct
      781 gtggctccaa gaatcattgc gtgtccggca attctctgtc cggtgacgtt tttattaggg
      841 tcgctgtagt gcgtattaaa ataatacaaa ccatcagaaa ctgtttttaa cggcattaaa
      901 gctgaaccgt caccaatcgg atttccagat ttcttgcccg aagaataatt atgcagtgaa
      961 aacgtacagg caagattggc tgccgttctt cgcgtactca gtggtcttgt tttattcatt
     1021 ggcagatgac tgaaaaacac ataaggtgcg gcactggttg cacgagaaaa acggaatcct
     1081 ttacccgtcg taataaactc actggatact ttgataccat tcttacgggc agcttctgcc
     1141 gtatcgccaa atactgttag tacgcaatga tatgaaccaa aaagattagt accgctggta
     1201 acaaaatctt tccctgctat cagttcttct tgttgagatt tagcagcatc accagttgac
     1261 cccattttat taagttggga gtctattttt cgcacggttt tagctggagc ctcaaacaaa
     1321 aatgattgcg taatgataaa ttcatacggt agttttaaca ggaaatccca ttgaccagga
     1381 gtggtaaaca tagggaaatc ttttaaaaca taatttgtgg caaatttttt atcatccgac
     1441 tcattattac gcagttctag cgtatcatta ccaaaatacc agtcactatc tgcgattgat
     1501 tcatataatg gtgttgatga tacaggaata atcgtatgct ttttattaat taagcatgac
     1561 aaataatccg ctgt
//
LOCUS       DABSYT020000307         1572 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.309, whole genome shotgun sequence.
ACCESSION   DABSYT020000307 DABSYT020000000
VERSION     DABSYT020000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.309"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>1572
                     /locus_tag="ID725_RS26345"
                     /old_locus_tag="pgaptmp_005269"
     CDS             <1..>1572
                     /locus_tag="ID725_RS26345"
                     /old_locus_tag="pgaptmp_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HGV7853638.1"
                     /translation="SGASLEGFISSGLEVCASRMGGTAGRYLSNVAKVIKLPLLDIGI
                     NIWSLYDSSLNHAKATTQIEYISTAIDVSFSSINTALSIGAIAYPPLAIAIVPITIFS
                     HEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKATGIIDLSNNQVLGNIYLD
                     MRENPPILHGEKSYNSGKNIGSHPDMTDREIMESRAYNFACTKLSDAGEPDIFGWGDK
                     EICNSMELSESQLANGYSNRQWPSQIPVIPEGIYNTVYLGYGETLRANTEVTLSSTGY
                     FYEIARAYTDDELSEPLLTVCNQHSHVIGGKEPLTIIIPAIEHGMLGSNLHMIERFKN
                     YNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRDFNLDLDLSDGRKQNFN
                     FHHPSGFYSGKVMSITQKGINAVVGTKLGYDKIRGNNLDNTFSLGSGGGVIYSGGGSN
                     TYFVPATLQDNLHIYISEKSNGNHIILGDMHSRLSIECHFSNNEREFIKMGYYNGCDV
                     ILESDTIQKIKSFAK"
BASE COUNT          532 a          235 c          315 g          490 t
ORIGIN      
        1 tatctggtgc ctcgcttgaa ggatttatat cttctgggtt ggaggtgtgt gcttcaagaa
       61 tgggtggcac cgcagggcgt tatcttagca atgtggcaaa agtaattaaa ttacctttgc
      121 tagatatagg cataaatatt tggtcattat atgattcttc attaaatcat gctaaagcca
      181 ccacacaaat tgaatatata tcaacagcaa tagatgtatc tttttcatct ataaatacag
      241 ctttgtcaat aggcgcaatc gcatatcctc ctttggcaat agctatagtt ccgataacta
      301 ttttttctca tgaagtaaaa aactatgctg tttatgtgaa tcaaattaat gagcgccata
      361 agttatggct cgaagctgaa aagtatctgg acaatggaag tgcaaaggtt ctaagcatta
      421 acaaagccac aggcataatc gatttatcaa ataatcaggt gttaggtaat atttatttag
      481 atatgagaga aaatcctcca atattacacg gtgagaaatc atataatagc ggaaagaata
      541 ttggtagtca tcctgatatg actgacagag agattatgga gtctagagca tacaattttg
      601 catgcacaaa actctcagat gcaggtgaac ccgatatttt cggttgggga gataaggaaa
      661 tatgtaattc aatggaatta tcagaatcac aattagctaa tgggtattct aaccgccaat
      721 ggccttctca gattccagtt attccagaag gaatttataa tacagtatat cttggatatg
      781 gagaaacatt gcgagctaat actgaagtta cattgagtag cactggttac ttttacgaga
      841 ttgcaagggc atatactgat gatgaactat ctgagccatt attaacagta tgtaaccaac
      901 atagtcatgt tattggtgga aaagaaccat taacaattat tattcccgct attgaacatg
      961 gtatgcttgg ctctaatcta catatgattg aacgttttaa aaactataat ttctcgatat
     1021 caggagggaa gggagggata aaattaatgg ttggtggtat tggtgattac aacatagaat
     1081 gtacccccgg tgttagaaat attatatcgt ttgaacaatt atcacgtgac ttcaatctgg
     1141 atttggattt atcagatggg aggaaacaaa attttaattt tcatcatccc tcaggttttt
     1201 attcaggaaa agttatgagc ataacgcaaa agggtataaa tgcggttgtt ggtactaaac
     1261 tgggatatga taagattcgt ggtaataatc ttgataatac cttttctctt gggagtggag
     1321 gaggtgttat atattctgga ggagggagca atacctattt cgttcccgcc acactacagg
     1381 ataatttaca catatatata tcagaaaaat caaatggcaa tcatattata ttaggtgata
     1441 tgcactctcg cctatctatt gaatgccact ttagtaataa tgaaagagag tttataaaga
     1501 tgggctacta taatggatgt gatgtgattt tagaaagtga cactatacaa aaaataaaat
     1561 catttgcaaa aa
//
LOCUS       DABSYT020000308         1564 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.310, whole genome shotgun sequence.
ACCESSION   DABSYT020000308 DABSYT020000000
VERSION     DABSYT020000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.310"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(67..141)
                     /locus_tag="ID725_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /pseudo
     CDS             complement(67..141)
                     /locus_tag="ID725_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(138..563)
                     /locus_tag="ID725_RS26355"
                     /old_locus_tag="pgaptmp_005271"
     CDS             complement(138..563)
                     /locus_tag="ID725_RS26355"
                     /old_locus_tag="pgaptmp_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGV7853639.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            650..865
                     /locus_tag="ID725_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /pseudo
     CDS             650..865
                     /locus_tag="ID725_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(908..1247)
                     /locus_tag="ID725_RS26365"
                     /old_locus_tag="pgaptmp_005273"
                     /pseudo
     CDS             complement(908..1247)
                     /locus_tag="ID725_RS26365"
                     /old_locus_tag="pgaptmp_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          391 a          389 c          364 g          420 t
ORIGIN      
        1 aacctctatt tctgggtatt tgttatcact taatccatta ctgctcctcc ttcagcatat
       61 gtcgtatccg ttgaaggatt tacgcatatc ggtaacgtcg gcaacgagcc agatgcgggt
      121 acctgacggg agtgagatca tcgtcccctc ccggtcagtt cacggatcaa cagcgtgagc
      181 agctctggcg atggattttc cagcgtcatg ttaccgtgac ggaactccac cttgcaggag
      241 ccggcactga cactggtctg agtggaagtg gataaagacg gcgcaatggc cgccaccggt
      301 tctttctgct catccggcgt tatttctaca ggtaataatt caacgccagt gtcagaagag
      361 gtcgttacct gaagacgccg tgatatacgc ccttcgttct gccagagcct gagccatttg
      421 aacaggaggt tatcattgat atcgtgttcc cgggcaatac gggcaacaga ggctcctggt
      481 tgtgatgcca gttccaccat acgaagtttg aattcattcg aatagttttt acgaggttct
      541 tttcgccagt cctgtaattc catacttaga tgtccgtcta tatcagatgg gcgtctaagt
      601 taccaatttt cgtctgatgg ctacatacgg cggtcagttt acgcttactg acaacgcgat
      661 ggcggagagc atcaatggtc tttacaaagc ggaggtaata caccgtaaga gctggaaaaa
      721 ccgtgcagaa gtggaactgg ccacactcac gtgggtggac tggtataaca atcgacgatt
      781 gctggaaagg ctgggccata ctcctccggc agaagcagaa aaagcttatt atgcttccat
      841 cggaaacgat gatctggcag cctgagttca cagataaaac actctccagg aaacccgggg
      901 cggttcaaac ctgattctgg aaaaggactt tgtggattgt tgctattgcc agtgagattg
      961 aatgaagtcg ttttaactcg ctttgaatac gtcttgcccc cagatttcgc tgagaacgta
     1021 actccagaat cagagcgact tcgcaagtgc cggttttcgt tgatggtgaa cgcttggggc
     1081 gacggctgtg gctttccaga ccagcgatgc cctgagccag atatctgcgc caccatttta
     1141 cgcaatgtgg gccgagaaat gccacagcga cggcatacaa atcccgcatc accggacgtt
     1201 tcatagagtt ttacccactg tagccgctgc tgaatttccc tgttcataat ttggcattag
     1261 ttcagctcaa tcccgactta aaaagtccct acaggatgtt ccggacctga ggcacagttc
     1321 atatccttat ccataaaaaa tttagctatg tctatagcat taatcttgac aagacgacgt
     1381 tagggcctgg cgatgaatca tttcccctgc cagatatcct ttggggtgca ccagaactac
     1441 cgttttatat ctatataact gaaacgtccc tcgccatcct tcatattttg atgtagtaac
     1501 accattcact ttcatctacc ccccgtttta ttaacatata gtaaaaatca cctcgccgca
     1561 tttt
//
LOCUS       DABSYT020000309         1550 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.311, whole genome shotgun sequence.
ACCESSION   DABSYT020000309 DABSYT020000000
VERSION     DABSYT020000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.311"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..>1550)
                     /locus_tag="ID725_RS26370"
                     /old_locus_tag="pgaptmp_005274"
     CDS             complement(<1..>1550)
                     /locus_tag="ID725_RS26370"
                     /old_locus_tag="pgaptmp_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGV7853640.1"
                     /translation="TYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEAI
                     VDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPKVVKGVSFMLRLT
                     VAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPA
                     APSQIELTPGYFQITAVPRLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTGLYWI
                     AASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQEL
                     WTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNS
                     MWAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADRIAMINPANGNTKPMFVGQ
                     GDQIFMNEVFLKYLTAPTITSGGNPPAFSLTSDGKLTAKNADISGSVNANSGTLNNVT
                     INENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIV
                     IPAVAFSG"
BASE COUNT          317 a          468 c          398 g          367 t
ORIGIN      
        1 gccgctgaat gccaccgccg gaataacaat ctgccggtca aacggctgat cgtcataaac
       61 cctgacggta atggtccctg atggccaccg ttccggtgca cgggagtccc gggggaaagc
      121 tttgcccact gttttaacga gatcgccttc aatctggttc gcggacagtt ttcccagaac
      181 ccgacagttc tcgttaatcg tgacgttgtt gagcgtcccg gagttcgcat tcacactgcc
      241 actgatatcc gcatttttag cggtcagctt tccgtctgat gtcagggaaa atgccggagg
      301 attgccgcca ctggtaatgg tgggagccgt cagatatttc aggaacactt cattcatgaa
      361 tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg ccattcgcag gattaatcat
      421 cgcaatcctg tctgccgcca gcagcacctg actctgcatg ccgtcagagg tgttctcaat
      481 accggcaccg atacccgcaa tataaaggcg tccgtcctgc atctgctgca gcttcactgc
      541 ccacatgctg ttcaggttat tatttgtatc aacctgaacc ttctgtatct gctggatcgc
      601 tgcactctgg tcttccagtt tcttattgac ggtctgcgtg atttcattac tgacatccgt
      661 gatggacgtt ctgatttccg ccaggtcagg cgcaagctga ccgttatcaa tctgagtcca
      721 caactcctga gccagatggg ttttccctat ctctcctttg aaaaaatcca gatagccgga
      781 tgcatcatca ctcggctggc caacagcctc cacaaatgcc gatttgccaa cggtgttcac
      841 actgcggata taaaagtaat aatcatggcc cggtttgata ttgatactgg cggctatcca
      901 gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc tgcctgatat cggtaatccg
      961 cgtttccgaa aaccagaact caaactgtac cgtcgggtca tacaccgcaa gacgcgggac
     1021 cgctgttatc tgaaaatagc ccggtgtcag ctcaatctgt gacggcgctg ccggtgcggc
     1081 aatccggaac gataccgacg ccggatcgcc ctgctgcccc cacgcattta ccgcccggac
     1141 tgtcagcctg tagttccccg gcgccagttg cgtgaagcgg tatgtggttt ccgtcgtccg
     1201 ggccgtgctg accagccgct cactgccgtc atccgcggcc acggtcaggc gaagcataaa
     1261 gctcacgccc ttcaccacct tcggcgtgtc ccatttcgcc tgcgccagat actgaccgtc
     1321 agctgcgctc acctccaccg tcaggtgctg cactgccgga gggatgacgc tgttcagggt
     1381 gcctgactgc ggctcaaagc gggcaccgtt atccacgatg gcttcttttt ccggtacgtg
     1441 ctgcaccgcc gtgatggcaa aggtgccgtc cgtgttttcc cggacggaga cacagcggaa
     1501 caggcgacgg cgcagtgacg gcagggagag cccccacaca ccgtatgtct
//
LOCUS       DABSYT020000310         1543 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.312, whole genome shotgun sequence.
ACCESSION   DABSYT020000310 DABSYT020000000
VERSION     DABSYT020000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.312"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            512..1063
                     /gene="ybcL"
                     /locus_tag="ID725_RS26375"
                     /old_locus_tag="pgaptmp_005275"
     CDS             512..1063
                     /gene="ybcL"
                     /locus_tag="ID725_RS26375"
                     /old_locus_tag="pgaptmp_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HGV7853641.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVASNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            1073..>1543
                     /locus_tag="ID725_RS26380"
                     /old_locus_tag="pgaptmp_005276"
     CDS             1073..>1543
                     /locus_tag="ID725_RS26380"
                     /old_locus_tag="pgaptmp_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGV7853642.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHTLTVDNNQIILFPCGYEFYIENYPEAGLYLAEMLYLPIDLIESFQKLYTVTDQIR
                     NKTSFFLPQNPELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLS"
BASE COUNT          483 a          242 c          328 g          490 t
ORIGIN      
        1 cgataagtgc tctcaaatat atggttgatg gtgagattta tttctaaata atgatctcgg
       61 attttaagtt atgctatgat gataaagtgc aagacagaat taattatctt taacgaaact
      121 taatgggtaa ttactttgtt tgctcccaca agcgagtttt gtacggctgt attggggtag
      181 taaatgagct atacaatctt aatcatttgt taggtgagaa ctcttggtcg cagattcaaa
      241 tactgaaaat acgtgacaaa ttattatgag caaaatggtg tatgtcacgt attttgaatg
      301 gtaggttaaa aaataacacc gactttcgta ggtattacta ataataaagc agagttttta
      361 gatagtatca atgtgctttg tgtatattgt ggcaaataat tgggttgggg gtacaattgt
      421 gattgctttt gcatgaacat tgcgccttta tgcataatga gataaaggaa tatcaaataa
      481 aataacgata ggtcataaca aagaggtttt tatgaaaaca cttatcgttt caactgtatt
      541 ggcattcata acattttctg cgcaggctgc agcatttcag gtcgctagta atgaaataaa
      601 aacaggagag caacttacaa cgtctcatgt cttttctgga tttgggtgtg aaggtggtaa
      661 tacatcgccc tcattaacct ggtctggtgt tcctgaaggt accaaaagct ttgccgtaac
      721 tgtatatgat ccagatgcac ctacaggcag tggttggtgg cattggactg ttgttaatat
      781 tccagcaaca gtaacatatt tgcccgttga tgcagggaga cgtgatggaa caaaactgcc
      841 gactggtgct gttcaaggcc gaaatgattt tggctatgct gggtttggtg gcgcatgtcc
      901 tcctaaagga gataaaccac atcattacca gtttaaagta tgggctctaa aaactgaaaa
      961 gattcctgta gattctaact ccagcggagc gttagttggt tatatgctta atgctaataa
     1021 aatcgcaacc gctgagataa caccagttta tgagataaag tagggtgaga gtatgctggc
     1081 aagaggtaag actaacttaa agatcgaaga aatacggatg cataaacatc atgagattca
     1141 tagggttaag cctcttatgc cagctttgtg tcgtatccgt cagggaaaga aagttatcaa
     1201 ttgggagacg catactttaa ctgttgataa taatcaaata atattatttc cttgtggtta
     1261 tgaattttat attgagaatt atcctgaagc agggctttat cttgcagaaa tgctttactt
     1321 acccattgat ttaattgaga gtttccaaaa gctttatacg gtaactgatc aaatacgtaa
     1381 caaaacaagt ttctttttac ctcagaatcc tgagttaata tattgttggg agcaactaaa
     1441 aacatctgtt tcccgaggct tctcaactaa aattcaggag cacttagcaa tgggcgttct
     1501 actttcgtta ggagtgaatc atgttaatca tttactttta tca
//
LOCUS       DABSYT020000311         1533 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.313, whole genome shotgun sequence.
ACCESSION   DABSYT020000311 DABSYT020000000
VERSION     DABSYT020000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.313"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(12..167)
                     /gene="hokD"
                     /locus_tag="ID725_RS26385"
                     /old_locus_tag="pgaptmp_005277"
     CDS             complement(12..167)
                     /gene="hokD"
                     /locus_tag="ID725_RS26385"
                     /old_locus_tag="pgaptmp_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGV7853643.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
     gene            772..>1533
                     /locus_tag="ID725_RS26390"
                     /old_locus_tag="pgaptmp_005278"
     CDS             772..>1533
                     /locus_tag="ID725_RS26390"
                     /old_locus_tag="pgaptmp_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGV7853644.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNERIIKIIKDDISESKHTKSICLLNGNLSLDYSASLYPEAIKAESV
                     LRNILTEKYSNFSIVQFPELNHQETFSAALWNSIIH"
BASE COUNT          466 a          319 c          285 g          463 t
ORIGIN      
        1 cgccaggtct cttactcctc aggttcgtaa gctgtgaaga cagcgacctc cgtctggccg
       61 gttcggactc gtacctcgca gaggtctttc ctcgttacca gtgccgtcac tatgacggtt
      121 aaacagatga cgatcagggc gattaacatc gccttttgct gcttcatagc ctgcttctcc
      181 ttgcctttcg gcacgtaaga ggctaaccta gatttgccgt tcatagattg agcctcagat
      241 taatgttagg cgtcttgcag gacgcgtaat gttaactggg gcttttctct atctgccgtt
      301 ggtgttcatg cccgaggcag atagcctcaa gcacccgcag ctattctact taacttccca
      361 ttacctcgcc aatatgaaat cggtcagaaa gacagacgtc ccataaggac aacaacggaa
      421 aaagagattt tgcttgatgc tatttacctc tgcaccatga agttgcgcca aaatcccagt
      481 ggtgataaca ggaatttggt tgttggtgat tggggaaaga agtacgttag actgtgggtt
      541 agttcattga catgatagtt acctgccttc aatttcctaa gcaagcgatg taaacgataa
      601 tcaatatcaa atgtattgac aggtaaaata ataacgatgt acctttgctg ataacatata
      661 tcagtcttaa atcataatgg aggtagataa aataactaaa cacacacaac aaatttttta
      721 tcagtgacat agcatatctt aagcatcctt taatgctcat ggggagaacg catgactaaa
      781 catatggaca ttcattgtca aacacgcata tttaactcaa ttaaccatga caccaaatat
      841 aaaatatgta ttttcactcc aaaaaacatt aaaaaaaata atgcaactct ttatatactt
      901 gatgggaata gtgccaacaa ttacatttct gatattctgc ctgttattga tgcactaccc
      961 aatccaccag tgttagttac acttggttat gaatcttgga ataaccttag cattcatcgc
     1021 cgtgcttacg attatactcc tgatggtgaa aatgctattg ttgataactc taaaccagca
     1081 tggatctact ttactggtgg aggaagccag tctttccgag aattattact aactcagatc
     1141 atgccatggg ttagtactat tgctccgaac agttccagaa tcggtatatg ggggcactcg
     1201 ctaggtgcta tttttgtgct tgattgctta aaaaataatt catgctttaa ctattattat
     1261 atatctgcac catcacttct atggcagaat gaaagaatca tcaagataat taaagatgat
     1321 atatcagaat caaagcacac taaaagtata tgcttgctta acggaaatct tagccttgat
     1381 tactccgcat ctttatatcc tgaagccatc aaagcggaat ctgttttgag aaatattttg
     1441 acagaaaaat acagtaattt ttctattgta caatttccag agctcaatca tcaggagact
     1501 ttctctgctg cactctggaa tagcattatt cat
//
LOCUS       DABSYT020000312         1531 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.314, whole genome shotgun sequence.
ACCESSION   DABSYT020000312 DABSYT020000000
VERSION     DABSYT020000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.314"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..222
                     /locus_tag="ID725_RS26395"
                     /old_locus_tag="pgaptmp_005279"
     CDS             <1..222
                     /locus_tag="ID725_RS26395"
                     /old_locus_tag="pgaptmp_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HGV7853645.1"
                     /translation="FRELYPALKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDI
                     DIYEPDGPTNLLAWERLKNKELPPSLHRY"
     gene            297..1019
                     /locus_tag="ID725_RS26400"
                     /old_locus_tag="pgaptmp_005280"
     CDS             297..1019
                     /locus_tag="ID725_RS26400"
                     /old_locus_tag="pgaptmp_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGV7853646.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSQHGNVRISIERLAKRGVIQLP
                     SMQKVENKQTISPNKFTSVYIFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKRFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            1019..>1531
                     /locus_tag="ID725_RS26405"
                     /old_locus_tag="pgaptmp_005281"
     CDS             1019..>1531
                     /locus_tag="ID725_RS26405"
                     /old_locus_tag="pgaptmp_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGV7853647.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNR"
BASE COUNT          483 a          344 c          379 g          325 t
ORIGIN      
        1 ttccgtgagt tgtatcccgc attaaaactg attcagtctg gctattccgg catatgccac
       61 gactacggct atgagttctc gtatatcatc gggagggcga ggggcgtttt aattaatcac
      121 acgcgggata tagatattta tgagcctgac gggccgacga accttctggc atgggaaagg
      181 cttaagaaca aagagttgcc gccttcactg catcgctact gacaattgac aacttaacaa
      241 acccagcttc ggctgggttt tttattgctg aattttcaat gtgagaggac atgacaatga
      301 atgagctgat aaatagcaat gccatcaaaa tgacaagcat tgaaatcgct gagttggtgg
      361 gaagccaaca cggtaatgtc agaatatcaa tagaacgtct ggcaaagcgt ggggtgattc
      421 aacttccttc aatgcaaaaa gttgaaaata aacaaacaat tagccctaac aaattcacaa
      481 gcgtgtatat attcgaaggc gaacaaggta agcgagacag cattattgtc gtcgctcagt
      541 tgtcgccgga attcaccgct cgccttgttg accgctggcg agaactcgaa ggggcaaccg
      601 cgaaaatacc acaaaccttt tctgaggcat tgcgccttgc ggccgacctt gaagaccaga
      661 aggctgaact ggagaaacag cttgctctcg cagcacctaa agttgagttt gccgatcgag
      721 ttggcgaggc cagcggaatt ttgattggaa actttgcaaa ggttgttggt attggtccaa
      781 acaaacggtt tgcgtggatg cgcgatcaca aaatccttat tgcttcaggt gcccggcgca
      841 atgtgccaat gcaggaatat atggatcgcg gctatttcac agtgaaagaa acagcggtca
      901 atacaaatca cggaatacag atatcgttca ccacaaaaat caccgggcgt ggtcaacagt
      961 ggctgacaag aaagctgcta gataacggaa tgcttaaagt aacaggggag gctgcttaat
     1021 ggctaaacca gcgcgaagga aatgcaaaat ctgtaaggaa tggtttcacc cggcattctc
     1081 aaatcagtgg tggtgcagcc cggaacacgg aactaaatta gcactcgaac gacgaaataa
     1141 agaacgcgaa aaggcggaaa aaacagcaga gaagaaacga cgacgagagg agcagaaaca
     1201 gaaagataaa attaagattc gaaaactcgc cttaaagccc cgcagttact ggattaaaca
     1261 agcccaacaa gccgtaaacg ccttcatcag agaaagagac cgcgacttac catgtatctc
     1321 gtgcggaacg ctcacgtctg ctcagtggga tgccggacat taccggacaa ctgctgcggc
     1381 gcctcaactc cgatttgatg aacgcaatat tcacaagcaa tgcgtggtgt gcaaccaaca
     1441 taaaagcgga aatctcgttc cgtatcgcgt cgaactgatt aaccgcatcg ggcaggaagc
     1501 agtagacgaa atcgaatcaa accataaccg c
//
LOCUS       DABSYT020000313         1503 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.315, whole genome shotgun sequence.
ACCESSION   DABSYT020000313 DABSYT020000000
VERSION     DABSYT020000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.315"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            206..385
                     /locus_tag="ID725_RS26410"
                     /old_locus_tag="pgaptmp_005282"
     CDS             206..385
                     /locus_tag="ID725_RS26410"
                     /old_locus_tag="pgaptmp_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGV7853648.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            426..671
                     /locus_tag="ID725_RS26415"
                     /old_locus_tag="pgaptmp_005283"
     CDS             426..671
                     /locus_tag="ID725_RS26415"
                     /old_locus_tag="pgaptmp_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGV7853649.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            749..964
                     /locus_tag="ID725_RS26420"
                     /old_locus_tag="pgaptmp_005284"
     CDS             749..964
                     /locus_tag="ID725_RS26420"
                     /old_locus_tag="pgaptmp_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGV7853650.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            969..1502
                     /locus_tag="ID725_RS26425"
                     /old_locus_tag="pgaptmp_005285"
     CDS             969..1502
                     /locus_tag="ID725_RS26425"
                     /old_locus_tag="pgaptmp_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGV7853651.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
BASE COUNT          380 a          339 c          451 g          333 t
ORIGIN      
        1 ggtgcgactt acccggtact gatagacagc attgcggtgg aagtgaacag cacagacact
       61 gcggcatgat aaaaaaaccg ccagcgacag gaatggacgc tggcggtggt aatacctatg
      121 gagaaaaaat aaaggaacga tactttcgtg ctctggtttt ttaaatgaaa acagttctta
      181 ttgtcaacaa taacggaaag aaattatgac atttctgaac cagttaatgc tgtacttctg
      241 tacggtggtc tgtgtgctgt atctcctttc gggtgggtac agggccatgc gtgacttctg
      301 gcgcagacag attgacaaaa gggccgctga gaaaatcagc gccagtcagt cagccggaag
      361 caaacccgaa gagccgctca tttagcggca actttcttaa tcacatcttt cgacgagaaa
      421 atcccatgtc agaaattaca tccctggtca ctgctgaagc agtgaaggaa gtcctgcgct
      481 ctgaagaagt ccggagcgca ctgaaacaga aacttcgcca taacctggaa gcgcgtcttg
      541 atgcagaagt ggatgccatt ctggatgaac tgctgggcgc accggcagct ccggagccgg
      601 aaggcatcgc gggtgagggg agtgcttcag atagcggtga ccccacaccg gacagcgaca
      661 tgatgatgta agcatgcgtc agggaccatc ggtgtgtgcc ggtggtcttt tttattgttg
      721 tgagcttccg gattgcggga gacggggtat gtaccagatg gaaaaaatca caacaggtgt
      781 gtcatacacc acgtcagcgg tgggaacggg ctactggttc ctgcagttgc tggacagggt
      841 ttccccgtct cagtgggcgg caataggcgt gctggggagt ctgctgtttg ggctgctgac
      901 atatctgacg aacctgtatt tcaagattaa agaagaccgg cgtaaggcgg cacggggaga
      961 gtaagctgat gagcaggaaa ctccgctatg gtttatcggc tgccgttctg gcgctgattg
     1021 ccgcaggtgc ttctgcgcct gaaatcctcg accagtttct ggatgaaaag gaaggtaacc
     1081 acaccacagc ataccgtgat ggtgcgggta tctggaccat ctgccgtgga gccacccggg
     1141 tggatggtaa gcctgttatt cctggcatga agctgtcgaa ggaaaaatgc gaccgggtta
     1201 acgccattga gcgtgataag gcgctggcat gggtggagaa aaacattaaa gtgccgctga
     1261 ccgaacccca gaaagcgggg atcgcgtcat tctgtccgta caacattggt cccggtaagt
     1321 gtttcccgtc gacgttttac agacgaatta atgcaggtga tcgaaaaggt gcctgcgaag
     1381 ctattcgctg gtggattaag gacggtggca gagactgccg tattcgttca aacaactgtt
     1441 acggtcaggt atcccgtcgt gaccaggaga gcgcgctggc gtgctgggga atcgacagat
     1501 aag
//
LOCUS       DABSYT020000314         1492 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.316, whole genome shotgun sequence.
ACCESSION   DABSYT020000314 DABSYT020000000
VERSION     DABSYT020000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.316"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            407..>1492
                     /gene="espX2"
                     /locus_tag="ID725_RS26430"
                     /old_locus_tag="pgaptmp_005286"
     CDS             407..>1492
                     /gene="espX2"
                     /locus_tag="ID725_RS26430"
                     /old_locus_tag="pgaptmp_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HGV7853652.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRDEKGITVR
                     IDNINGKKNNSNSSDVFIPYVNTFPDLKNKILRMKIELTEGSGFSKSLSDSQIEMHIL
                     RTVNSLNVGEKLNDDNLADHSIFTNEFSVIIPPSYYDATSAVNANNIVREKLFESDSK
                     VKDIVDDMSNHDVESEKDIFVIGGMIEKLNSLADESFNDSTDNIQTVKDLLTQLTDGM
                     ELFALRDIVAFPSTIIAKLIKSPLNSDHELVMRALDTYLCYFRNKNLNNNAEIINFFH
                     ALFLKRPELMVAENYRFIQFIDLLFENG"
BASE COUNT          495 a          241 c          280 g          476 t
ORIGIN      
        1 tctgggaaaa tctggaataa acatgctatt gcataaagag tttgttcatc atctggtaaa
       61 ttattatgag atatgtgatt cagtattagc ttgattctgt ccctgatttt attattttca
      121 ttgtctatag tgtatccgac tatctcccta agcaaaataa agtgtgcagt cgtttcatta
      181 ccgtatatct ttagtagttc taataaacta tcaaaaatcg ctcatttcag cttattgata
      241 gaaagattga ttgccagtct gtttagagcc gtacagcagc aaactttcat aaccaggttg
      301 atcctgaatt tgcagtacga aaagttaaaa atgagcgatt tctgtaagac tccccatttt
      361 gccaggctta tgattcgcag atcattcaag tgcaaaggag cgatgtatgg attgttcaaa
      421 atgcaatggt tatgcgacga tgttattaaa tatggtgcag ggttctgacc cggttaattt
      481 actggaatta catggtttcc ttgaacattt cgcatattat gtttcatttg gcaaatttaa
      541 tgctggccac cagcgctata atgcatttaa aaaattcgtc agtgagattt cggagatcag
      601 cgcaaatgat atcaatatga cgattaaaac cgggcaatcc agacatgaaa atgtgatctc
      661 tataaatatg aatgatgcaa ttcctcgtga tgagaaaggg atcaccgtta gaatagataa
      721 tattaatgga aagaaaaaca actctaattc atcagatgtt tttattcctt atgtaaatac
      781 atttcccgac ctcaaaaata aaatactcag gatgaaaatt gaactgactg aaggtagtgg
      841 tttttctaaa agtctttctg acagtcagat tgagatgcat attctgagga ctgttaattc
      901 gctaaatgtt ggcgagaaat tgaatgatga taatcttgca gatcattcta tattcactaa
      961 cgaattctct gttattattc cgccatccta ttatgatgca acttctgcag taaacgctaa
     1021 taatattgtg agagaaaaac tatttgaaag tgatagtaaa gttaaagata tcgtcgatga
     1081 tatgtctaat catgacgtgg agagcgaaaa agatatattt gtaatcgggg ggatgataga
     1141 aaaactgaat tctctggcag acgaatcttt caatgatagt actgataaca tacaaacagt
     1201 aaaagatctg ttaacgcaat taactgatgg catggagctc ttcgcgctgc gggacatagt
     1261 ggcattccca tcaaccataa tagcaaaatt aataaaatcc ccacttaata gcgatcatga
     1321 acttgttatg cgcgcactgg atacgtatct gtgctacttc agaaataaaa atctgaataa
     1381 caatgcggaa ataattaact ttttccatgc cctcttttta aagagaccag agttgatggt
     1441 tgctgaaaat tatagattta ttcaatttat tgatttgctt tttgaaaacg ga
//
LOCUS       DABSYT020000315         1467 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.317, whole genome shotgun sequence.
ACCESSION   DABSYT020000315 DABSYT020000000
VERSION     DABSYT020000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.317"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..470
                     /locus_tag="ID725_RS26435"
                     /old_locus_tag="pgaptmp_005287"
     CDS             <1..470
                     /locus_tag="ID725_RS26435"
                     /old_locus_tag="pgaptmp_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGV7853653.1"
                     /translation="ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVY
                     QKREIAVDADYDHDRIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDE
                     FVTCCFTEQGCKDYLAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            472..648
                     /locus_tag="ID725_RS26440"
                     /old_locus_tag="pgaptmp_005288"
     CDS             472..648
                     /locus_tag="ID725_RS26440"
                     /old_locus_tag="pgaptmp_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853654.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     RNSASCLIGEMFSC"
     gene            982..1338
                     /locus_tag="ID725_RS26445"
                     /old_locus_tag="pgaptmp_005289"
     CDS             982..1338
                     /locus_tag="ID725_RS26445"
                     /old_locus_tag="pgaptmp_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853655.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     WECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYA
                     EGWNDCREAMLQGDKS"
     gene            1335..>1467
                     /locus_tag="ID725_RS26450"
                     /old_locus_tag="pgaptmp_005290"
     CDS             1335..>1467
                     /locus_tag="ID725_RS26450"
                     /old_locus_tag="pgaptmp_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853656.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     "
BASE COUNT          432 a          292 c          377 g          366 t
ORIGIN      
        1 aagagtgtgc tgcgatggct gaacgtatcg aagagatgca gacaaaatct gcaccagatt
       61 cgtttggcat catcggtgaa aatattcgaa cacaggacaa tcgaataacg tcagatccca
      121 tgttttgtgt gtatcaaaag cgcgaaatcg ctgttgatgc tgattatgac catgaccgga
      181 ttgtctgggt tgacgaagat ggcaatgaag ccaataaacg ccatagtcgt cgtctcgagc
      241 tacttcatga aaactttcga gagccaccag aaaaatggcg gcgcgttgct gtgaaagata
      301 ttgatgaatt cgttacctgc tgtttcaccg aacagggttg taaagactac ctggcagtca
      361 atggtcacaa tcttcgcttg ccatttatat atgtaaaaag cggtttcagg aacgctgaat
      421 atatcggcat aagaaactgg cttgctggca ttcgcatcaa aggagagtga tatggcgtta
      481 acacaccgcg aactctgtca gattgcgtac aagttcctta agcgcaacgg tttcaaggtt
      541 tgttttcatg accgctttat agctgtaacc agtaccggag aacagccaga tgctatggga
      601 ttcagaaatt cagcatcatg cctgattggc gaaatgttct cgtgctgact tgttggcaga
      661 tagaaaaaag cgttttcgta aaaatccgtc tcttggaatg ggcgactggc gattctttat
      721 tagtgagccg ggaattattt caattgagga tttaccacct ggctggggat tacttcacgt
      781 tgttaacgga agagtacgga aagtacatgg gtggcccaag ggtaattgct gttggggtaa
      841 tcctgacgat aagccattta ctggaaataa gcaggttgaa tgcgattaca tgttgtctgc
      901 attaaggcgc atggagttga gagggcacct taatgaaata tatgacggtg taattgttaa
      961 taagaaagaa ggaaacgcgg catgaccact attaccgaca aagaactgat taaagaaatc
     1021 aaagagcgca taggcagcct ggacgttcga gacaatattg agcgccgtgc ttatgaaatt
     1081 gcactggcat cgctggaagc agaaccgata gcgtgggaat gcggtgaaaa cataatcctg
     1141 tttaaccctg acacagttga agcatacgca aaacgtgcgg aaatatcacc taaaccacta
     1201 ttctccgcgc cgccagcgct ggtagtgcct gataagttgc cgcgtgaata cagaaacggt
     1261 tggcctcttg cgtatagtga ttatgctgaa ggctggaacg actgccgcga agccatgctt
     1321 cagggagata aatcatgatt aatcgtatca agctggagca catcctcgaa tatgccaggc
     1381 agcagaggca tattggtcag cattgtaaaa ttccaccagg agatatggtt gaaatcatgg
     1441 agattgccat gcgcaaggct ggcaact
//
LOCUS       DABSYT020000316         1458 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.318, whole genome shotgun sequence.
ACCESSION   DABSYT020000316 DABSYT020000000
VERSION     DABSYT020000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.318"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..221)
                     /locus_tag="ID725_RS26455"
                     /old_locus_tag="pgaptmp_005291"
     CDS             complement(<1..221)
                     /locus_tag="ID725_RS26455"
                     /old_locus_tag="pgaptmp_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853657.1"
                     /translation="MDNLIGTPPNHAVPHNYIDMEQMEYLCHLNRFSKLSNDFLINGP
                     REDLFTYKYVLDGSFSNLQHLLPKGQLQK"
     gene            522..>1458
                     /gene="espY4"
                     /locus_tag="ID725_RS26460"
                     /old_locus_tag="pgaptmp_005292"
     CDS             522..>1458
                     /gene="espY4"
                     /locus_tag="ID725_RS26460"
                     /old_locus_tag="pgaptmp_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY4"
                     /protein_id="HGV7853658.1"
                     /translation="MVAKLKPDVFVNTNPFLEAMYKERLSHKGYSDKIALSINDKKYN
                     VNSKDIENILDGKGDLFKKRTLWEFVRDLFPGSHIKEVKGLIYEFVTKVDNKAEVFDK
                     IKSLAKKEQQWRFSTKTDFTTNENNEVIVSRSFNLYTGATSNDNEKKQVSSERLTLDN
                     YLDDLHFDNSPLVRLTFDNYSVKLATLIKNKIPIINTTINLSSLSKDVLNSLKYCSFK
                     NVIFSGVINSPNLEGPVFENCYFDDCKFNNIQLYETNDDTVESGNKKPIKGMFKGCFI
                     SKCKIENYRCETSKIYTVTQPDSIQEKLGSYLFMQS"
BASE COUNT          522 a          212 c          245 g          479 t
ORIGIN      
        1 atcttctgta gttgtccttt aggaagtaaa tgctgtaaat ttgaaaatga accatctaaa
       61 acatatttat atgtaaaaag gtcctctctt ggaccattta ttaaaaaatc gttagacaat
      121 ttagaaaaac gattgagatg acataaatat tccatttgct ccatgtctat atagttatgg
      181 ggaactgcgt ggtttggtgg tgtgccaata agattatcca taaatcacct tgataaaatt
      241 taataaaatc tctttatttt aacctcgaca ataaaagcgg ctataaaaaa acgctcattt
      301 tctgatgctg cggttaaccc gtggaatgta ccgttaaccc ccaaaaaatt gcaaatccag
      361 ggtgaacacc ctgggttaaa atggaaataa tgagctattt tttacagtat tcaccggtaa
      421 tataagtaac acttgttgct aacctaacta gcagtacagt taattaacaa catagaagtt
      481 attgtttttc aaacaactca atattacacg ccggaggatt tatggtcgct aaattaaaac
      541 cagatgtttt tgttaatact aatccttttt tagaagcaat gtataaggag aggcttagtc
      601 ataaaggcta ttctgataaa attgctttaa gtattaatga taagaaatat aatgtaaact
      661 ctaaggatat tgaaaatatt cttgatggca aaggggattt atttaaaaaa cgtacactgt
      721 gggagtttgt tcgcgatctt tttcctggct ctcatattaa agaagtcaaa ggcttaattt
      781 acgaatttgt cacgaaagtt gataataaag ccgaggtgtt cgataagatt aaatctctgg
      841 caaaaaaaga acaacaatgg cgatttagta ctaagacaga ctttaccaca aatgaaaata
      901 atgaagttat tgtgagtaga tcctttaatc tctacacggg ggctacttcg aatgataacg
      961 aaaaaaaaca ggtttcttca gagagattaa cgcttgataa ttatctcgat gatttacatt
     1021 ttgataattc tcccttggtg agattaacgt ttgataatta ttctgtaaaa cttgcgacgc
     1081 tgattaaaaa taaaattcct attattaata caacaatcaa tttatcatcg ttgtctaagg
     1141 atgttttaaa ttcattaaaa tattgtagtt ttaagaatgt aattttttca ggagtgataa
     1201 acagccctaa ccttgaaggt ccggtttttg aaaattgcta tttcgatgat tgtaaattca
     1261 acaacataca gctttatgag actaatgatg atactgtgga atcaggaaat aaaaaaccaa
     1321 taaaaggtat gtttaaaggt tgttttattt ctaaatgcaa aattgaaaat tatagatgtg
     1381 agacgtctaa aatctacacc gttacacaac ctgatagtat tcaagaaaaa ttaggttctt
     1441 atctctttat gcagtctt
//
LOCUS       DABSYT020000317         1458 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.319, whole genome shotgun sequence.
ACCESSION   DABSYT020000317 DABSYT020000000
VERSION     DABSYT020000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.319"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..405
                     /locus_tag="ID725_RS26465"
                     /old_locus_tag="pgaptmp_005293"
     CDS             <1..405
                     /locus_tag="ID725_RS26465"
                     /old_locus_tag="pgaptmp_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853659.1"
                     /translation="EAIEHGKNEINETAVLAKEKLNNEIIDIISLLRKNQEELNNSYK
                     KSIAEFSKNTKPFSKSTAIALCVACTIGISAAFSGAFWYVAQSQKEQALQFYASGYMD
                     MQKLTKETISLLPKEQQKIATAKLNALESRSR"
     gene            complement(434..871)
                     /locus_tag="ID725_RS26470"
                     /old_locus_tag="pgaptmp_005294"
     CDS             complement(434..871)
                     /locus_tag="ID725_RS26470"
                     /old_locus_tag="pgaptmp_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGV7853660.1"
                     /translation="MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVM
                     AREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQDYSHCDSWFVIDVDLSEIEGKQQ
                     RINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK"
     gene            complement(896..1078)
                     /locus_tag="ID725_RS26475"
                     /old_locus_tag="pgaptmp_005295"
     CDS             complement(896..1078)
                     /locus_tag="ID725_RS26475"
                     /old_locus_tag="pgaptmp_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGV7853661.1"
                     /translation="MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKK
                     DLPLGTVRSIRKMAGI"
BASE COUNT          486 a          349 c          260 g          363 t
ORIGIN      
        1 gaagccatag agcatggcaa aaatgaaatc aatgagacag cagtcttagc gaaggaaaaa
       61 ttaaacaatg aaataataga tattatatcc ttactaagaa aaaaccagga ggagctaaac
      121 aactcttaca aaaagagcat tgctgaattt tcaaaaaaca caaagccatt tagtaaaagt
      181 acagcaatag cattatgtgt agcctgcacc attggaattt cagccgcttt ttctggtgcg
      241 ttctggtatg ttgcgcaatc acaaaaagaa caggcgctac agttctatgc atccggctac
      301 atggatatgc aaaaactaac aaaagaaacg atctctctac tacctaaaga acagcaaaaa
      361 attgcaaccg ctaagttaaa tgcactggaa agccgcagta ggtaaaaaaa tccccgcatc
      421 aagcggggat ttttcattta tatgaaagtt catgccgcgc ggcctgagca agaaaatggc
      481 tacgatctct gtaaacaccg ccactttctc taacaaatcc atcaatacga cgaatcaaca
      541 catcaggcaa cgcaatatta atacgttgtt gtttaccttc aatttcagac aaatcaacat
      601 caattacaaa ccagctatcg cagtgactat agtcctgatt tgcggcatat gttaaacagc
      661 cgtcatcatg aatatcgtca acagaataag agccacttaa aaccatttct tccacgatag
      721 ccaatatcgc ttctcgcgcc atcacaggaa tttctgattg agtatcggca gcagagacac
      781 agccaaaatc aaaacgatca aaagccggaa cggttatacc atatgccgta tggtcatctt
      841 tcggtgtttc gacacccaca gaaaaaaaca tgattacctc caaagtcggc ggggattaaa
      901 tccccgccat ttttctgatc gatctgacag tacccagtgg caagtctttt ttcggatggg
      961 gcacaggaaa agtttttcct gttatgggcg accaccatat ctggtggctg ctcgccttat
     1021 gccgcttcaa ctcacaacca gcagctatca gttctttcaa caggtcagca gatttcatat
     1081 gtcctcccta acctgccact aattatacac acatatacac acaaaagcaa aaaagaagat
     1141 taatttcgtg cgtaactgct ctatgcaaca ttgaaaaaat tccgcttcct cgccactaac
     1201 tcgctacgct cggtcgttcg gctgcggcga gcggttaaaa aaacaaatga tgtgtattaa
     1261 tacacaaaaa aaagggggag aataatctcc cccccccttc acatcaaact aaagcattat
     1321 cccggttatg cgcccaccaa atgatttcgc taataatcca tgcttctatt gctggggttt
     1381 tgacttgttc cccgtttaca atccggttgg caagctctat catttcgcac actgacattt
     1441 gaaaatctcc aaagatac
//
LOCUS       DABSYT020000318         1456 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.320, whole genome shotgun sequence.
ACCESSION   DABSYT020000318 DABSYT020000000
VERSION     DABSYT020000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.320"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(70..1185)
                     /locus_tag="ID725_RS26480"
                     /old_locus_tag="pgaptmp_005296"
     CDS             complement(70..1185)
                     /locus_tag="ID725_RS26480"
                     /old_locus_tag="pgaptmp_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853662.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFSHKEHPTPKLYKDEQYLDKQIEG
                     LFSTLLTINDNFLRAKAEIASTIIKFLEARITNLSYNDILKYQQEFQKECYKQVKSFT
                     TLSRYNKIQTWAEMSEYQFEVFQYETLNPKKCHARLI"
BASE COUNT          396 a          231 c          274 g          555 t
ORIGIN      
        1 ttcttttgcc agatggtatt tctatttcca ccccataatt tatatctttt tcattaggaa
       61 gtggcctctt taaataaggc gtgcgtgaca ttttttgggg ttaagcgtct cgtattgaaa
      121 tacttcgaat tgatattcag acatttctgc ccatgtttga attttattgt atcgagagag
      181 tgtcgtaaag gatttaactt gcttataaca ttctttttgg aattcttgct gatatttcaa
      241 tatgtcgttg tatgaaagat tggttatcct ggcctctaaa aatttaatta ttgtcgaagc
      301 aatttccgct tttgctcgta aaaagttgtc attaatggtt aataatgttg aaaataaacc
      361 ttcgatttgt ttgtcaagat attgttcgtc tttatataat tttggagtag ggtgttcttt
      421 atgtgaaaaa atagtgtcac ctaaatttgc gtcagtgaaa tttatattaa gtagatcttc
      481 gaaataaaac cttgacaagg ttagaacgaa cccggacagg ttctgtcctg tcatattgaa
      541 atcaataaag ttggttccgg caaagacttt catcctgttt ttattttcac tgtgctcaat
      601 atataacttc gtcatttttt gatacaggct ttgaatatct tttatattag tcggtaagtc
      661 agctcgttgt tcagcaataa aattagtatc tgaaaacatg gtttggacac tgaatcttac
      721 tggtattccc tcttctacgc taattttagt tatgattgcc ttgtcgggtt taggattagc
      781 gacaacaatg attcgacatt cagcactttt gttcttttcg tatctaatgt tattaatttg
      841 attaagtatt tcctgtgcct gccgcgtaag tggtgtcgaa cttatcgatt caatgttagt
      901 aataagactt acaagttgag ccacaacttt tgagtagtct gcatctgtat atttggtgtt
      961 cgtgcggcac gcataaattt gtgggaggtg ttgccgcgct aaatcatatt gttcagggtt
     1021 gtatccttct ccagagaatg tggaattaat gggttgttgt tcttcccgat tatcacaagg
     1081 ccacatcggt gtgtctggtg ttttaacatt gtcggtatct gttgtgcggt tataaatatc
     1141 gccgtgatat gtatttatca tacctggggt aaagtcaatt gacataacat tccttattca
     1201 ttatctgttt tatgctaaga tttttaaatt ggataattaa agcaggaatg gattttcttc
     1261 tacgcacaat aaatatcatg cgtcatatct tcctgatata attacctttg acttcccttt
     1321 tatctccacc cggtaatatt cgtgtataaa aaagggcatt gatagtatcg taaaggaaaa
     1381 cgttaacaac tatcaaaaag cgctcatttt aaaaagttat gatgttctcc gatagtgtgt
     1441 gaaatgatat ttttgt
//
LOCUS       DABSYT020000319         1450 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.321, whole genome shotgun sequence.
ACCESSION   DABSYT020000319 DABSYT020000000
VERSION     DABSYT020000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.321"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..801
                     /locus_tag="ID725_RS26485"
                     /old_locus_tag="pgaptmp_005297"
     CDS             <1..801
                     /locus_tag="ID725_RS26485"
                     /old_locus_tag="pgaptmp_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGV7853663.1"
                     /translation="VLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIPV
                     PRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIPVPRDG
                     RDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRGTYATHQGGLWRA
                     YEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFR
                     AGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDAGG"
     gene            804..1130
                     /locus_tag="ID725_RS26490"
                     /old_locus_tag="pgaptmp_005298"
     CDS             804..1130
                     /locus_tag="ID725_RS26490"
                     /old_locus_tag="pgaptmp_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGV7853664.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1140..>1450
                     /locus_tag="ID725_RS26495"
                     /old_locus_tag="pgaptmp_005299"
     CDS             1140..>1450
                     /locus_tag="ID725_RS26495"
                     /old_locus_tag="pgaptmp_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGV7853665.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GG"
BASE COUNT          313 a          350 c          499 g          288 t
ORIGIN      
        1 gtactgcaga aggcggtgaa tgatgcggtc gcaaatattc cgcagccggc ggacggtaaa
       61 agtctcaccc cggatgatgt gcgtccgatg cttgaacaga tggtgaagga ggcggtaagc
      121 catatccctg ttccgcgcga cggtcgtgac tatgatccgg aagtactgca gaaggcggtg
      181 aatgatgcgg tcgcaaatat tccgcagccg gcagacggta aaagtctcac cccggatgat
      241 gtgcgtccga tgcttgaaca gatggtgaag gaggcggtaa gccatattcc tgttccgcgt
      301 gatggtcgtg actacgatcc ggatgttctg cagaaggcgg ttctggatgc ggtgagtgcc
      361 ctgccggctc cgcaggacgg gcgtgatgcc acggcactgg aaatactccc cgccattgac
      421 gatcaaaaat cctttccccg gggcacgtat gccacacacc agggcggact ctggcgggcg
      481 tatgaaaaaa cgcacgggat gcggggatgg gaatgcctgg ttgacggggt ggcggatatt
      541 gacgtcagca tgacgggtga gcggttgttc tctgtggtgg tccggcagag cagtggccag
      601 cgtacggaaa aaacattttc cctgccggtg atgctctacc gcggtgtgtt cagagccggt
      661 gaaacctacc accccggcga tacggtgacg tgggggggct cgctgtggca ctgcaacagt
      721 atgaccgaag ataaacccgg agaagctcat tcatcagcct ggaccctggc tgcaaaacgt
      781 gggcgggatg caggaggctg aaaatgacgg cattactgac actggaagag atcaaggcac
      841 atctgcgtgt tgaccatgac gcggatgatg acatgctgat ggacaaggtt cgtcaggcta
      901 ccgccgtgct gctggcctac attcagggca gccgggataa ggtgattcgt gaggacggtg
      961 aactgatccc gggcgaggca ttaacccgga tgaagggggc tgccatgcga ctgaccggga
     1021 tgctgtaccg gaatccggat cttgcggagc gggaagaact gattcagggg gagctgccgt
     1081 tttctgtttc cgtgctgatt tacgatttgc gttgtccgac ggtgttatga ggagggggaa
     1141 tggcaatatc tgcaggtcgt ctgacacaga tgatcagtgt tctgaacccg gtgttaaccc
     1201 gtaacgctgc cggagaaatg acggaagaat gggtgtcatg cgggaaaatt catgcggata
     1261 tccggggcag gagcagccgg gagcggatgc agtccggtgc ggaaatggcg caggcggaaa
     1321 tccgcatctg ggtgcgcggt cagtccggtc gggaaatcac ggcagcgtca cgacttcatg
     1381 tgctgagtgg tccctggcgt gaccggatcc tgaacgttgt cgggctgccc gtgccggatg
     1441 cgaccggcgg
//
LOCUS       DABSYT020000320         1441 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.322, whole genome shotgun sequence.
ACCESSION   DABSYT020000320 DABSYT020000000
VERSION     DABSYT020000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.322"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..555
                     /locus_tag="ID725_RS26500"
                     /old_locus_tag="pgaptmp_005300"
     CDS             <1..555
                     /locus_tag="ID725_RS26500"
                     /old_locus_tag="pgaptmp_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGV7853666.1"
                     /translation="ALSMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGT
                     VIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLG
                     ASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSR
                     RISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(746..1273)
                     /gene="sodC"
                     /locus_tag="ID725_RS26505"
                     /old_locus_tag="pgaptmp_005301"
     CDS             complement(746..1273)
                     /gene="sodC"
                     /locus_tag="ID725_RS26505"
                     /old_locus_tag="pgaptmp_005301"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGV7853667.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          329 a          353 c          427 g          332 t
ORIGIN      
        1 gccctgtcga tgcagatgcc gggattccgc cgtcagatga acgaaggctg gtaccagata
       61 cgtattgccg gtgatgacac ggcaccggag gcggtgtacg cccgtcttca cgaacagctg
      121 ggtgagggaa cggtcatcca cattgtgccg cgactggccg gggccggaaa gggtggactg
      181 cagattgtgc tgggggcggc ggccatcgtg gggtcgttct tcactgccgg ggcatcaatg
      241 gcgttatggg gttcagccct ggcagccggt ggtttttctg ccaccacgat gctgttttca
      301 cttggagcca gcatgattct gggcggtgtg gcccagatgc tggccccaaa ggcaaaaaca
      361 ccggattacc gcgcaacgga taacggcaga cagaacacgt acttttcctc gctggataac
      421 atgattgccc aggggaaccc gatgccggtg ccttacggtg aaatgctggt tggctcccgg
      481 cgaatctccc aggacatcag tacccgtgat gaaggcggtg acgggaaggt ggtggttatc
      541 gggcggcagg cataaaagcg aaaaaatccc gcagtgctca cggacaggaa ctgcgggagc
      601 gttacgaaga ttgagtgtaa ggaattattc ttatgtcacg acaaaaacat taactcagag
      661 agggaggatg tgccgttctt ttcagggaga aaggatttat cgtcctgagg aataaaggta
      721 aggggcccgc cccttacctg actgattatt gaatgatgcc gcaggccatt ctcgcaccac
      781 caccgcccag gggctccgga tggtcatgat ggttatcacc gccagcatga agcatgagag
      841 aacgcccttt aatctctttt aatgagttca gtctcggggc caggaccggg tagttcgctt
      901 ttccgtcatg cgtcacgaac agcgcaggga ggtcgcccag gtgtccatcc ggagaccagg
      961 ggccaagatg tttgccggtg tttttcgggt caaagtgacc gccagccgat aatgctgcga
     1021 ccggttttcc gtctttcagt gccggggcgc aatttccttt ttcgtgcaca tgaaaaccat
     1081 gaatgccttc agacagagag tgaagggctg gtgtgaacag cagaccgtag ggggtctcct
     1141 gaatggttat ttttccaatg ctgacttctt ttccgtcagc actgacaagg ttcattggga
     1201 cttcctgttc tgctgcgtat ccgcatgatg ctgctgtcag catggcaatg gcagcaatga
     1261 ttttacattt cataaaaccc tcattaattc cgttaacaga ctgagcttgc tggttacagg
     1321 gtaacaaaca gcgttctgat gatatcgcgc aatagctgtg caatatccta tcactgcgat
     1381 taataatacc aattgagagg aacattatgg gtaaaggtgg cggcagggcg cacacgccgg
     1441 t
//
LOCUS       DABSYT020000321         1401 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.323, whole genome shotgun sequence.
ACCESSION   DABSYT020000321 DABSYT020000000
VERSION     DABSYT020000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.323"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(1..566)
                     /locus_tag="ID725_RS26510"
                     /old_locus_tag="pgaptmp_005302"
                     /pseudo
     CDS             complement(1..566)
                     /locus_tag="ID725_RS26510"
                     /old_locus_tag="pgaptmp_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            complement(772..>1401)
                     /locus_tag="ID725_RS26515"
                     /old_locus_tag="pgaptmp_005303"
     CDS             complement(772..>1401)
                     /locus_tag="ID725_RS26515"
                     /old_locus_tag="pgaptmp_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGV7853668.1"
                     /translation="GETEVVSNLIKLGVDLDARGDLGRTALNEAVSNGYFDIVKLLVE
                     SGADLTIKDEFGKTALELAEVHQNYKIVEWLSHYRDHNPIPDFSVLINIYGERYFGGE
                     IIDTDARTELGEGVLHIAVRKRRQLDVRCFIQHGADINAKANNGFDFTPLHYSIGIGD
                     FDMMKLLLKLGADIYLESEMGYSPMDLAILMGDKRIIFYLYGYISHVSE"
BASE COUNT          343 a          394 c          275 g          389 t
ORIGIN      
        1 gccggtagcg tcacacccgt gtcctcctgc aacacctccg ccaggctacg gtgccgcgcc
       61 ttcgcctgtt caccattctc tgtttcgatt ctcaggagcg gcgtgaagct tcccggctgg
      121 tataccgtct ggattcttgt ctgttgggtc tgtacagtgg tcagcctgtc accgtcccac
      181 ccgtaccagg tctcctccgg tttacgcgac agcgacatcc accccgtcag gtcacgctcc
      241 cgccgccaca cccgttttcc cgtccggcgg cccagcgggt catagaggta ccggctctcc
      301 acctgtggtt cgccgtgctg tatccgcgtg tggaacacca ggcggtgctg gctgtcgtag
      361 tgatagtggt gggtgcgctc gtcgtgcatc cggataaccc cttccgggat gcggtccgtc
      421 ttctccgcca gcctgccgta ttcatcgtag cggtagacat agtgcgcatc ttccgcgatg
      481 cggttatccg gccatgccgt gagggtgctg tccgggtgca gttccgggtc cggcagccgg
      541 tttcctgccg ggtctgttgc atacgggaaa ttcccttgca gggcgtccac aacatgtgca
      601 tatttcctgc acatgctgtg gttttgtcat acaaggagca acaatactag ttaatgattc
      661 ataaaaaatt ttaacggggt gatttttcat gcgattatcg tcctaattaa cgccacctca
      721 cttacagatt atttcattcg ttacaatctt ttgattgcat tacacccagg cttattcaga
      781 aacatgagaa atataaccat ataaataaaa aataattctc ttatccccca tcaaaatagc
      841 caaatccata ggggagtatc ccatctcact ctccaaataa atatcagcac ctaatttcaa
      901 caaaagcttc atcatatcaa aatcccctat ccctatcgaa taatgcaaag gagtgaagtc
      961 aaagccatta tttgccttag catttatatc ggcaccatgt tgaataaaac atctgacatc
     1021 taattgtctt cttttcctaa cagctatatg aagaaccccc tcgccaagtt ctgtccttgc
     1081 atctgtatct ataatctctc caccaaaata tcgctcaccg tagatattaa taagtacaga
     1141 aaaatcgggt atgggattat gatctctata atgtgatagc cattcaacta ttttataatt
     1201 ctggtgtacc tcagccaatt ctaatgccgt tttcccaaat tcatccttta tagtaagatc
     1261 ggctcctgat tctactaaaa gcttaacaat atcaaaatac ccattactta cggcctcatt
     1321 aagtgcggta cgccccaaat ccccacgagc atccagatcc acacccaatt taattaaatt
     1381 acttacaacc tcagtctcac c
//
LOCUS       DABSYT020000322         1369 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.324, whole genome shotgun sequence.
ACCESSION   DABSYT020000322 DABSYT020000000
VERSION     DABSYT020000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.324"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..1237
                     /locus_tag="ID725_RS26520"
                     /old_locus_tag="pgaptmp_005304"
     rRNA            <1..1237
                     /locus_tag="ID725_RS26520"
                     /old_locus_tag="pgaptmp_005304"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          338 a          309 c          424 g          298 t
ORIGIN      
        1 aactaggcaa aatggtgccg taacttcggg agaaggcacg ctgatatgta ggtgaagtcc
       61 ctcgcggatg gagctgaaat cagtcgaaga taccagctgg ctgcaactgt ttattaaaaa
      121 cacagcactg tgcaaacacg aaagtggacg tatacggtgt gacgcctgcc cggtgccgga
      181 aggttaattg atggggtcag cgcaagcgaa gctcttgatc gaagccccgg taaacggcgg
      241 ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacctgcac
      301 gaatggcgta atgatggcca ggctgtctcc acccgagact cagtgaaatt gaactcgctg
      361 tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg tgaaccttta ctatagcttg
      421 acactgaaca ttgagccttg atgtgtagga taggtgggag gctttgaagt gtggacgcca
      481 gtctgcatgg agccgacctt gaaataccac cctttaatgt ttgatgttct aacgtggacc
      541 cgtgatccgg gttgcggaca gtgtctggtg ggtagtttga ctggggcggt ctcctcctaa
      601 agagtaacgg aggagcacga aggttggcta atcctggtcg gacatcagga ggttagtgca
      661 atggcataag ccagcttgac tgcgagcgtg acggcgcgag caggtgcgaa agcaggtcat
      721 agtgatccgg tggttctgaa tggaagggcc atcgctcaac ggataaaagg tactccgggg
      781 ataacaggct gataccgccc aagagttcat atcgacggcg gtgtttggca cctcgatgtc
      841 ggctcatcac atcctggggc tgaagtaggt cccaagggta tggctgttcg ccatttaaag
      901 tggtacgcga gctgggttta gaacgtcgtg agacagttcg gtccctatct gccgtgggcg
      961 ctggagaact gaggggggct gctcctagta cgagaggacc ggagtggacg catcactggt
     1021 gttcgggttg tcatgccaat ggcactgccc ggtagctaaa tgcggaagag ataagtgctg
     1081 aaagcatcta agcacgaaac ttgccccgag atgagttctc cctgaccctt taagggtcct
     1141 gaaggaacgt tgaagacgac gacgttgata ggccgggtgt gtaagcgcag cgatgcgttg
     1201 agctaaccgg tactaatgaa ccgtgaggct taaccttaca acgccgaagg tgttttggcg
     1261 gatgagagaa gattttcggc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa
     1321 aacagaattt gcctggcggc cttagcgcgg tggtcccacc tgaccccat
//
LOCUS       DABSYT020000323         1360 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.325, whole genome shotgun sequence.
ACCESSION   DABSYT020000323 DABSYT020000000
VERSION     DABSYT020000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.325"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            52..186
                     /locus_tag="ID725_RS26525"
                     /old_locus_tag="pgaptmp_005305"
     CDS             52..186
                     /locus_tag="ID725_RS26525"
                     /old_locus_tag="pgaptmp_005305"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HGV7853669.1"
                     /translation="MDNATGPRLADTAERDYFILRERLMTMQKQLEGAQDYIRTQCLN
                     "
     gene            complement(549..776)
                     /locus_tag="ID725_RS26530"
                     /old_locus_tag="pgaptmp_005306"
     CDS             complement(549..776)
                     /locus_tag="ID725_RS26530"
                     /old_locus_tag="pgaptmp_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGV7853670.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            818..1183
                     /locus_tag="ID725_RS26535"
                     /old_locus_tag="pgaptmp_005307"
     CDS             818..1183
                     /locus_tag="ID725_RS26535"
                     /old_locus_tag="pgaptmp_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGV7853671.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
BASE COUNT          322 a          314 c          366 g          358 t
ORIGIN      
        1 atcaacgcca cctgctccgg taccgtgcgt gaagccaccg gcacctccgg cgtggataat
       61 gcaaccggcc cccgactggc agacaccgct gaacgggatt atttcatcct cagagaacgg
      121 ttgatgacaa tgcagaagca gctggaaggg gcacaggact atatccgcac tcagtgcctg
      181 aactaagttt tgctgatgcg ccgtatcgtc gccgtattcc tgcattaaca gagaccgcag
      241 cccgacaggg agactcctct gcgagagtgt gcggggataa tcaaaaacga tacacaccgg
      301 ggtttaccgc gtaaacggag cgcggcgttc tcccctcatg gtcgcccgtc cggtgcgatg
      361 gtggaagaaa ctggaatctg ttcaataaaa aaactgccgt gttggagtca cagcagtaat
      421 gtactgattg ggtagaagat tattattgtt atgctttatt tttattctat atggctgatt
      481 atttcaattc ggaattaata cagctaatgt ctgtgagttt ttataaattc agcaatataa
      541 agaaatagtt atatgaacag ccatcgcaga gcatactgtg tatcattctt ttttatagtc
      601 aactgacggg catattttat gtctgctgcc agctcccggc ggcaagattc aatgacccac
      661 gcagaaaaat tttctgaacc tttctggtca agagcgatgt taatttgttc aatcatctgg
      721 tttggaaatc ggatgttgcg ggttgttgtt ctgcggggcc ggtttttcga tgacattttc
      781 tttcctctgg tgacaagcta tatggcgagg attttacatg gctgtgcttc gtacgttacc
      841 gggcagaatc aaaactctga acacccggcg ggtgaatatt ctgaagggtg aacagcgtcg
      901 tgtcagtggc agtgcacgtg tttccctcaa gcgtcatatc tggctcaggg atgccgggca
      961 gtgctgtctc tgtggtcgtg tggttgacct ctgtgacagt gaactcgatc accgaattgc
     1021 acttcagttc ggtggtggta atgaggagac gaatctctgg acgctctgta ccgaatgcca
     1081 tcgacaaaag tctgctcgtg aagcggcggg tggtatgccg gacccgacgc tgccggaggt
     1141 gtccggaggt catggcaggg cagacgatat catcggactg tgacccgccc cggggggggg
     1201 gatcatccgg cgaaaaaaac gatcgctccg gacaccgccc cccgtctcac gcagagaaaa
     1261 aattcccgtt tcagggcagt taacatgtta actggctgtc cgggcatttt tgcggttttt
     1321 atctttatta ttcagtttgt tgtgcggaaa aaatgttaac
//
LOCUS       DABSYT020000324         1360 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.326, whole genome shotgun sequence.
ACCESSION   DABSYT020000324 DABSYT020000000
VERSION     DABSYT020000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.326"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            180..>1360
                     /locus_tag="ID725_RS26540"
                     /old_locus_tag="pgaptmp_005308"
     CDS             180..>1360
                     /locus_tag="ID725_RS26540"
                     /old_locus_tag="pgaptmp_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HGV7853672.1"
                     /translation="MVDNVTVSRVCIQSPSFVPDLDGEKNKSQLFVDDIVAYLKSPSV
                     YSLEKEGPLNHFVNHCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPVLIKFLDDIFTCLHTYPDDESFTKEQIKANSKYDIVDYNCLLHFTGKPKSLIEC
                     RHFALQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFNNLQKNPPPGTCVVA
                     SDKFGEALSVFFHRMEKEKLTHMTAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLQSFINIQQAKQKWVITDICSECVGITPYLPREQAHLLSG
                     IENELQPPLSPPALFLLMRMGIYKNIVLFFDKLKNSQEMTASKALDILAAKSPEGIYG
                     L"
BASE COUNT          452 a          239 c          252 g          417 t
ORIGIN      
        1 caaggagctg ttcccctcgt tgacgtcgcg gtttacccac ctcattacta ggttgatgac
       61 ctgatgggga aatgacgtaa aaagacaaat aactctataa atgagcgaaa tttgatagtt
      121 gatttttaaa tagtaacctt attattacta caactttcat atggatgagg ggaaagaaaa
      181 tggttgataa cgtaactgtt agcagagtat gtatacaatc tccttctttc gtgcctgatt
      241 tggatggaga aaaaaataaa tctcaattat tcgttgacga tatagttgcg tatcttaaaa
      301 gtccttcagt ttattcactt gaaaaagagg ggccgttaaa tcattttgtt aatcattgtt
      361 ctgaagttga gttaggtttc tatagcgatg gtgcatattc aattcttgtc tccagatcta
      421 agcaacaacc tgaaggtatg attttaaccg taagcgatgc agacgcaatc aatatagtac
      481 atatttccgt atctccagtg cttataaaat tcctggatga tatttttact tgccttcata
      541 cgtaccctga tgatgagagt tttacaaaag agcagataaa agctaatagc aaatatgata
      601 ttgtagatta taattgcctg ttgcatttta ctggaaaacc aaaaagttta atagaatgta
      661 gacattttgc tctgcaatac tgtatagatt caatgaatga gcatacaggg aaagttccat
      721 taaaggctta ctattcatct ccggaagata tacaaaaaca tattcctttc gagcttgagc
      781 agcaatttaa caatctacaa aaaaatccac cacccggtac atgcgtcgtt gccagtgata
      841 agtttgggga ggcattatct gtcttttttc acagaatgga aaaagagaag ttaacgcata
      901 tgacggcaat cgttcaatct caaacacacg ctatggccgt ccgcttgagg atcaaaaaaa
      961 cgcctgctgg tgaaacagaa tatgttgtat ccttttatga tcctaatgca accaatactg
     1021 cagtacgcta taaagcaaac aactgtgatt cttttgggtc attgcaatcg tttataaata
     1081 ttcaacaggc aaaacaaaaa tgggtaataa cagatatttg ctccgagtgt gtaggaataa
     1141 ccccttatct ccctcgggaa caagcccatt tattaagtgg tattgaaaat gagttgcaac
     1201 ctccattatc accaccagca ttatttctat taatgagaat ggggatatat aaaaacattg
     1261 ttcttttttt cgataaatta aaaaactctc aagaaatgac agcatcaaag gctcttgata
     1321 ttcttgctgc gaaatcacct gaaggaatat atgggttatg
//
LOCUS       DABSYT020000325         1346 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.327, whole genome shotgun sequence.
ACCESSION   DABSYT020000325 DABSYT020000000
VERSION     DABSYT020000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.327"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            1..186
                     /locus_tag="ID725_RS26545"
                     /old_locus_tag="pgaptmp_005309"
     CDS             1..186
                     /locus_tag="ID725_RS26545"
                     /old_locus_tag="pgaptmp_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGV7853673.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQTRIPTASQNRSPAPVF
                     VVMSGEYLLDKKEEIIQ"
     gene            199..288
                     /locus_tag="ID725_RS26550"
                     /old_locus_tag="pgaptmp_005310"
     CDS             199..288
                     /locus_tag="ID725_RS26550"
                     /old_locus_tag="pgaptmp_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853674.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            308..>1346
                     /gene="nleL"
                     /locus_tag="ID725_RS26555"
                     /old_locus_tag="pgaptmp_005311"
     CDS             308..>1346
                     /gene="nleL"
                     /locus_tag="ID725_RS26555"
                     /old_locus_tag="pgaptmp_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HGV7853675.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSDI"
BASE COUNT          402 a          227 c          288 g          429 t
ORIGIN      
        1 gtgaacacgg cagcggaagc catccgtgcc ctgtcgctgc agatgccggg attccgccgt
       61 cagatgaacg aaggctggta ccagacacgt attccgacag cttcacaaaa ccggagtccg
      121 gctccggttt ttgttgtcat gtccggggaa tatttgttag ataaaaaaga ggagataatt
      181 caatagggag ttaaattaat gccgataaat ctgacatctt atttgtgggt acagggacag
      241 aaagttgtcc cggcagttgt gttttctaat tttactttag tggactaagt aaaaaggagt
      301 gagataaatg ctgcccacta caaatatctc tgtaaattct ggagtaatat cttttgaaag
      361 tcctgtagat tcaccatcta acgaggatgt tgaagttgcc ctcgaaaagt ggtgtgctga
      421 gggagaattt agcgaaaatc gtcatgaggt tgcatcaaaa atacttgatg ttataagtac
      481 taatggagag actttatcaa tcagtgagcc aataacaaca ttaccagact tgcttccagg
      541 ttctctgaaa gaactggttt tgaatggatg tacagagctt aaatcaataa actgcttacc
      601 ccccaactta tcttcattaa gtatggttgg atgctcatca ttagaggtta taaattgcag
      661 catacctgaa aatgtcatta atttatcttt atgccattgt agttctttga aacatataga
      721 aggttccttt cctgaggcac tcagaaattc cgtatattta aatggctgta attcattaaa
      781 tgaatcgcaa tgtcaattcc ttgcatatga tgtcagtcaa ggccgtgcct gcctgagcaa
      841 agctgagctt actgctgact taatttggtt gtcagctaac cgaacgggtg aagagtctgc
      901 tgaagaattg aattactctg gatgtgactt gtcaggtcta agtcttgtag ggctgaattt
      961 atcatcagta aatttttctg gagcagtgct tgatgataca gatctcagga tgagtgattt
     1021 gtctcaggct gtattggaaa actgttcttt taaaaactcg attttgaatg aatgtaattt
     1081 ttgttatgct aatttatcta attgtattat tagggctttg tttgaaaact ctaatttcag
     1141 caattccaat cttaaaaatg catcatttaa aggatcttca tatatacaat atcctccaat
     1201 tttgaacgag gctgatttaa caggagctat tataattcct ggaatggttt taagtggtgc
     1261 tatcttaggt gatgtaaagg agctctttag tgaaaaaagt aataccatta atctaggagg
     1321 gtgttacata gatctatctg acatac
//
LOCUS       DABSYT020000326         1319 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.332, whole genome shotgun sequence.
ACCESSION   DABSYT020000326 DABSYT020000000
VERSION     DABSYT020000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.332"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            93..656
                     /locus_tag="ID725_RS26560"
                     /old_locus_tag="pgaptmp_005312"
     CDS             93..656
                     /locus_tag="ID725_RS26560"
                     /old_locus_tag="pgaptmp_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGV7853676.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            653..>1319
                     /locus_tag="ID725_RS26565"
                     /old_locus_tag="pgaptmp_005313"
     CDS             653..>1319
                     /locus_tag="ID725_RS26565"
                     /old_locus_tag="pgaptmp_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGV7853677.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQ"
BASE COUNT          313 a          340 c          413 g          253 t
ORIGIN      
        1 ccaggcagca gttaacattt gcggcatgag acgccgggaa aaatgggctg aaccataccc
       61 ggctgagtgc gttctggacc cgggaggagg ctgtgctgac aacgcaaaaa cgaaaatttg
      121 cgctggcgct catgtccggg aaaaacaaaa cagcgtcagc ccttgccgcc ggttattcgg
      181 cgaagacagc cagggttaaa ggctcgcagc tggcaaaaga tccggaggtg cttgcgttta
      241 tagcccgtaa acagtgcgag acggtggagg tggatgaggt tcctgtttat cggcagaaaa
      301 aatcagagcc ggaggataaa ccccgtcgcc gtgaggcggc tgcaatacca cagccggacg
      361 aaacaaatcc ggagatgcca ccgcccgtgg tgatatctcc tggtattgag tatatggagg
      421 acggtcttcc cgatccggtg aaagcgatgg ggcgtcttct ggtggagaac attaataccg
      481 accccaggct ggcgctggat gcggcttata agctggcgca gttcacgcac cacaaaaaag
      541 gggatgccgg taaaaaatcg gcaaaaggtg acgcggcgaa aaaagcggct aaccgttttg
      601 cggtgccacc accaccccgc ctggtggtga ataatgataa tgagggcaac ggatgatacc
      661 tgtgtggagc acggcctgcc cggactgggc agagcgcctg aaaaaggggc tgtcgattat
      721 tccggctccg atttatccgg agcaggccgc acatgccctg gcgattttta aacaactgcg
      781 gattgtggat gcaccgggca gcccgacgtt cggggagtcc tgcgcacagt gggtgtttga
      841 cctggtggcg gccctgtttg gctcctacga tgcgcagacc ggtgtacgcc atatcaagga
      901 agtttttatc cttatcccca agaaaaacag caagtccacg ctggctgccg ggatcatgat
      961 gacggcgctg ttactgaact ggcggcaggc ggcgggctac accattctgg ccccgaccgt
     1021 ggaggtggcg gctaacgcct tcaaccctgc cagggatatg gtacgacggg acgatgatct
     1081 ggatgacctc tgtcaggtgc agacacatat ccggaccatc acccacaggg tgacggacac
     1141 caccctgaag gtggtggctg ccgatccgaa tacggtatcc ggtatcaagt ccgtggggac
     1201 gctgattgat gaactgtggt tatttggcaa gcagtacaaa gcggaggaca tgttacgtga
     1261 agccataggc ggccttgcct cccgcccgga agggtttgtg gtgtatacga ccacccagt
//
LOCUS       DABSYT020000327         1318 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.333, whole genome shotgun sequence.
ACCESSION   DABSYT020000327 DABSYT020000000
VERSION     DABSYT020000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.333"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            206..1165
                     /locus_tag="ID725_RS26570"
                     /old_locus_tag="pgaptmp_005314"
     CDS             206..1165
                     /locus_tag="ID725_RS26570"
                     /old_locus_tag="pgaptmp_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HGV7853678.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          487 a          249 c          233 g          349 t
ORIGIN      
        1 taaaattata aaaaacataa aacaccacgc aaacacactt aataaaaaca ccgttacatt
       61 aaaagataat aaaaaccgca ataaaaaacg aataaatcaa ttgtctcacg caattataaa
      121 acatcatatt gattacgcac cttgcattac aaactcatgt atgtaaaata cgcgcaccat
      181 tcaaaaaaaa ggaagacaat aacatatgaa aaaaagtgtc atcgctggcg tctttattgc
      241 tctgtcattt accacgtgtt cagctatcgc gaacagcctt gcattatcat tagcaaatga
      301 tgatgcaggg aagtttcaac caatacttaa tgatatttat ggcaataagc atgaaaacag
      361 agatgattac tcacaaggct tatttctggg atatagccac gatatctcag actcgagcca
      421 attatctctc catattgcgc aagatattta ctctccatca ggcagtaata aaagacacaa
      481 cacagctgta actggagaca gagcttttag tgcatacact cacactggta ttgaatggaa
      541 ctcccttgcg aatgactgga ttcgctatcg attaggtact gacataggtg ttgttggccc
      601 cgacgcaggc ggtcagaaag tacaaaataa agctcatgag attattgggg cagaaaaata
      661 tcatgcatgg gatgatcaaa tagagaatcg ctacggttat actgtaaaag ggatgctatc
      721 catgacacca agtatggata ttttaggtgc taatgttgga ttataccctg aagtttctgc
      781 tgttactgga aacttatttc aatatgtagc atatggcgca accattgcca ttggtaatga
      841 taaaaccttc aattcggata atggctttgg tctgctggct ccccgtggtt taatgcatat
      901 gtccgataca agcggattca aatacaagat ttttgcaggt atggaaagac gagatgtcaa
      961 tcgcaactat actctcgaag gaaaaacaat acagacgaaa caaacaacag tatcgctaaa
     1021 caaaactgtt gatgaatatc aagttggcgc aacaattggg tatgcacctg tagccttcac
     1081 actagcattt aataaagtaa catcagaatt caagacaggg gatgactatt catttataaa
     1141 tggagcaatc accttctttt tttaactgaa ttgaattcaa tcaaaataac ataagtccaa
     1201 caaaaacata aagtgcgaaa tgaatgccag ctccatttat ttcgcactat aaaagattaa
     1261 aagttgcaat aaaataataa aatgactcag ttacgaaaac caataaactg tggccagt
//
LOCUS       DABSYT020000328         1286 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.334, whole genome shotgun sequence.
ACCESSION   DABSYT020000328 DABSYT020000000
VERSION     DABSYT020000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.334"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(23..>1286)
                     /locus_tag="ID725_RS26575"
                     /old_locus_tag="pgaptmp_005315"
     CDS             complement(23..>1286)
                     /locus_tag="ID725_RS26575"
                     /old_locus_tag="pgaptmp_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease SbcC"
                     /protein_id="HGV7853679.1"
                     /translation="IESIDDEINRIDLVLEELVAGLSALDKNMKADSIQYTELKDAHN
                     FISLKYKECILNITALEDKIDKYSRLKNDYDNDIEKLKAIKTANSRIGVLDKEVFSCP
                     VCDSHIKIDDSDFPFEISSEKDLNEELNSLSRRRRNINDMISEMSFKLKKESGYKKEL
                     DERLDELRELIDEESQSMITPFLTQRDFYIKEISKNEKVREQLVKDLKVRNQQEELLE
                     KYQVLRKDIEALTERLEALKKNAPSMEGILQGLGDRFSQYLKGINIKNRTGIKISESY
                     YIPVIREKEYFKITSGGLRTISSIGYLLSILDYAIDHNVNHPLLLIFDTVGKYLGKQT
                     KEKYSKETLAFEDDLEGMSDPMKYQNIYEQLLNTVQKAERKNVPCQIILVDNDLPNSF
                     STGELSNIIAHYSSVGEDGLPLGLIDDL"
BASE COUNT          399 a          255 c          164 g          468 t
ORIGIN      
        1 tcaaaaaaat gcatgtatag tattaaaggt catcaattaa acctaaaggc aaaccgtcct
       61 caccaaccga gctataatga gcgataatat tcgaaagttc accagtagaa aacgaattag
      121 gcaaatcatt atcaactaat ataatctggc acggaacatt ttttctttct gctttttgaa
      181 ctgtattcaa tagttgttca taaatatttt gatacttcat cggatctgac attccctcaa
      241 ggtcatcctc gaatgcaaga gtttcttttg aatatttttc cttggtctgt ttccctaaat
      301 atttgccaac cgtatcaaat atcaaaagta aaggatggtt aacattatga tctattgcat
      361 aatcaagaat agacaaaaga tacccaatag aagaaatagt tctcaaacct ccggatgtta
      421 ttttaaaata ttctttttct cttataacag gaatgtaata gctttcagag atttttatcc
      481 ccgttctgtt tttgatatta atacccttca aatactgact aaatctatca cccaaacctt
      541 gtaaaatgcc ctccatgcta ggtgcgtttt tctttaacgc ctccaagcgt tcagttaatg
      601 cttcaatatc ttttctcagt acctgatact tttcaagcag ttcctcttgc tggtttctaa
      661 ccttcaaatc ttttactaac tgctccctaa ccttctcatt ttttgagatc tctttaatat
      721 aaaaatctct ctgagtcaag aatggtgtaa tcattgactg gctttcttcg tcaatcaatt
      781 ctctcaactc gtcaagtcgt tcatctaatt cctttttata acctgattct ttttttaatt
      841 taaatgacat ttctgagatc atgtcattaa tatttcgtct acgtcgacta agtgaattca
      901 actcttcatt aagatctttt tctgatgaaa tttcaaatgg aaaatcagaa tcatctattt
      961 taatgtggct atcacagact ggacaggaaa atacttcctt atccaataca ccaatgcgag
     1021 aatttgctgt tttaatcgct ttgagttttt caatatcatt atcatagtca ttttttaacc
     1081 gcgaatattt atcaatctta tcctcaagcg cagtaatatt taatatgcac tctttgtatt
     1141 taagcgaaat aaaattgtga gcatctttca attctgtata ctgtatggaa tccgctttca
     1201 tatttttatc gagagcagac aaacctgcaa caagttcttc taaaacaaga tctatcctat
     1261 taatctcatc atcaattgat tcaatc
//
LOCUS       DABSYT020000329         1278 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.335, whole genome shotgun sequence.
ACCESSION   DABSYT020000329 DABSYT020000000
VERSION     DABSYT020000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.335"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..531
                     /locus_tag="ID725_RS26580"
                     /old_locus_tag="pgaptmp_005316"
     CDS             <1..531
                     /locus_tag="ID725_RS26580"
                     /old_locus_tag="pgaptmp_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGV7853680.1"
                     /translation="VEWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIE
                     MLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVF
                     IHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHY
                     MAGIFNYLKYYKEKCF"
     gene            557..1264
                     /gene="rfaY"
                     /locus_tag="ID725_RS26585"
                     /old_locus_tag="pgaptmp_005317"
     CDS             557..1264
                     /gene="rfaY"
                     /locus_tag="ID725_RS26585"
                     /old_locus_tag="pgaptmp_005317"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HGV7853681.1"
                     /translation="MIYNKTINGVKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
BASE COUNT          469 a          174 c          233 g          402 t
ORIGIN      
        1 gttgaatggt ggcaaaatcg agcctcggta ttaactacac cacagttagc ttctggatat
       61 ttcaatgctg gttttttact gataaatatt gatgagtgga atctaaataa catttcgtca
      121 aaagctattg aaatgttgcg tgacccagat tgggtaagta aaatcaccca ccttgatcaa
      181 gatgtactga atgtattatt gaatggtaaa gtgaagttta tttcggagaa atataatacc
      241 cgatatagta ttaactatga attaaaagac aaagttgata atccagtcaa tgatgacacc
      301 gtgtttatac actatgtggg acctacaaaa ccttggcatg agtgggctga ctatccggtg
      361 tcacgtagtt ttttgatcgc caaagcagct tctccgtgga gtaaagaaga tttacttaaa
      421 cctgtaaata gcaatcagta tcggtattgt gcaaaacata aatttaaaca aaagcattat
      481 atggcaggca tttttaatta tttaaagtat tataaagaaa aatgcttcta aagatttaag
      541 gttaaaataa ttcattatga tatataataa aacgataaat ggggtgaaag ttttcataaa
      601 agataatgat cccttttatg agcaagttct aaatgacttt ttaacttgca gggttaaaac
      661 attaaaagtt tttcgttcta ttgatgatac taaagtcatt cttattgaca ctgctcgtgg
      721 acctcttgta cttaaagttt atgcgccaaa gcataaaatg accgaacgtt tcttaaaatc
      781 ttgcataaaa aaagattatt atgaaaattt aatttatcaa accgatcggg tacgtggtga
      841 aggcatccaa tcaattaatg actatttttt gctagctgaa cgtaaaacat taaatttcgc
      901 tcattattat attatgctca ttgagtatat tgaaggtgtc gggttaaatg agtatttgga
      961 aatttctgaa gatctgaagg atcaattatc agaatcgata aaagagttgc atcagcatgg
     1021 aatggtgtct ggcgatcctc ataagggaaa ttttatagta tccgaaaaag gtttaagact
     1081 aattgacctt tcaggtaaaa aaacaaccgc tgttttaaaa gcaaaagatc gcattgatct
     1141 tgaacggcac tataatataa aaaatgaatt gaaagacttt ggctatacat atcttatatt
     1201 caaaaagaaa attaaaaaag taatcaggga tgtaaaagtc aaactaggat tgaagagcaa
     1261 ataatgatta taatgaag
//
LOCUS       DABSYT020000330         1277 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.336, whole genome shotgun sequence.
ACCESSION   DABSYT020000330 DABSYT020000000
VERSION     DABSYT020000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.336"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            6..81
                     /locus_tag="ID725_RS26590"
                     /old_locus_tag="pgaptmp_005318"
     tRNA            6..81
                     /locus_tag="ID725_RS26590"
                     /old_locus_tag="pgaptmp_005318"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ID725_RS26595"
                     /old_locus_tag="pgaptmp_005319"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ID725_RS26595"
                     /old_locus_tag="pgaptmp_005319"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ID725_RS26600"
                     /old_locus_tag="pgaptmp_005320"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ID725_RS26600"
                     /old_locus_tag="pgaptmp_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGV7853682.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT          324 a          334 c          304 g          315 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctg
//
LOCUS       DABSYT020000331         1271 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.337, whole genome shotgun sequence.
ACCESSION   DABSYT020000331 DABSYT020000000
VERSION     DABSYT020000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.337"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..225
                     /locus_tag="ID725_RS26605"
                     /old_locus_tag="pgaptmp_005321"
     CDS             <1..225
                     /locus_tag="ID725_RS26605"
                     /old_locus_tag="pgaptmp_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGV7853683.1"
                     /translation="LIEITESERQLLINQANEYMNSKQWPGKAAIGRLKGEELAQYNL
                     WLDYLDALELVDTASAPDIEWPTPPAVQAR"
     gene            complement(197..799)
                     /locus_tag="ID725_RS26610"
                     /old_locus_tag="pgaptmp_005322"
     CDS             complement(197..799)
                     /locus_tag="ID725_RS26610"
                     /old_locus_tag="pgaptmp_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGV7853684.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAETQRQSLIDTAMASISLIQLKLQAG
                     RKLTQAETNRLNTVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(799..>1271)
                     /locus_tag="ID725_RS26615"
                     /old_locus_tag="pgaptmp_005323"
     CDS             complement(799..>1271)
                     /locus_tag="ID725_RS26615"
                     /old_locus_tag="pgaptmp_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853685.1"
                     /translation="LPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKVYPTNKLP
                     DLRGEFIRGWDDGRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTIL
                     KQITPLSPAINFDNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
BASE COUNT          315 a          311 c          303 g          342 t
ORIGIN      
        1 cttattgaaa ttactgaatc agaaagacag ctactaatta accaggccaa cgaatacatg
       61 aacagtaagc aatggcccgg taaagccgct attggtcgtc tgaaaggtga ggaactggca
      121 caatataatt tgtggctgga ttatctggac gcactggagc tggtcgatac cgccagtgcg
      181 ccagatattg aatggcctac gcctccggca gttcaggcca gatgacatcc ggcgcggtgc
      241 tggtatctgt tgccgtcacc gcgtcaatgt aatccagcac cgtgttaagt cggttggttt
      301 ctgcctgcgt cagcttccgt ccggcctgta atttcagctg aatcagacta atggaagcca
      361 ttgcagtatc aatcagcgac tggcgttgtg tttctgccgc ttctactgcg gcgctatgct
      421 gtgcttcggt atccgtcacc catttctcac catcccattt atcgtatggc gttaacgggg
      481 cgatagtggt tgtattatca gggtaatcac ccggagcggt gatttctttt gattctcctg
      541 tttcggtgct aaagacgatt tcaccgcgat ggtctggcac atattcccat gagttaaaat
      601 ctgcagaacg gcagattgca taaccagctt tgtatgcgcc tggggcatct aaacaggaat
      661 atgccggaat gccgacacca atagcaagat attcagttga agctgaaata tattcccgac
      721 tcatgacatc aaagttataa acagtaattt ctcctgccat agtggcaatt aattcactgt
      781 ttaattctgc gttattcatt atgcagccct cacaatatag ttaaaagcga catttcgtgg
      841 gcgtgtttca ttcgcacctg caggtttagt cgttgccgca acaacaccac cctctgtaat
      901 agccgggttg ggaattgggt aattatcgaa gttaatggcc ggagacaggg gcgtgatttg
      961 ttttaaaatc gtggtgcctg taaaactttc cagagtctgg tcgttagttc gtgaagtcca
     1021 gattccccaa tagtggttat gactgacaat cgcaccgtcc tgaagcgtca ataatcccct
     1081 tccgttatcc actccacgtc cgtcatccca gccacgaata aactcaccac gtaaatcagg
     1141 caatttattt gtcgggtaaa cctttgccag ttccgggtat tcttcagcag aaaaagccgc
     1201 cccgttgcat ttcagccagc ctgtcggcgg agtggctgaa ggccacggaa caggcacacc
     1261 aacgggtaat g
//
LOCUS       DABSYT020000332         1246 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.338, whole genome shotgun sequence.
ACCESSION   DABSYT020000332 DABSYT020000000
VERSION     DABSYT020000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.338"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..240
                     /locus_tag="ID725_RS26620"
                     /old_locus_tag="pgaptmp_005324"
     CDS             <1..240
                     /locus_tag="ID725_RS26620"
                     /old_locus_tag="pgaptmp_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGV7853686.1"
                     /translation="PTHEELIEITESERQLLINQANEYMNSKQWPGKAAIGRLKGDEL
                     TQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
     gene            complement(212..805)
                     /locus_tag="ID725_RS26625"
                     /old_locus_tag="pgaptmp_005325"
     CDS             complement(212..805)
                     /locus_tag="ID725_RS26625"
                     /old_locus_tag="pgaptmp_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGV7853687.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGRVAVFTGKGWSHEEDHRNETVYSIENGAAVTVDYIGAIKDGYVT
                     ISPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(805..>1246)
                     /locus_tag="ID725_RS26630"
                     /old_locus_tag="pgaptmp_005326"
     CDS             complement(805..>1246)
                     /locus_tag="ID725_RS26630"
                     /old_locus_tag="pgaptmp_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853688.1"
                     /translation="APAIGVPFFWPSAAMPNTVIDSWSCMVFLKFNGAKFSATDYPVL
                     AKVFPSLVLPEARGDFIRIWDDGRGADGGRELLSWQAATNFSQFAGNIGEGAGHAINF
                     HDGIAGNQPGFSRFNFTSNSVGDGVNFVAVRPRNIAFNFLVRAK"
BASE COUNT          331 a          326 c          278 g          311 t
ORIGIN      
        1 cccacgcatg aagagcttat tgaaattact gaatcagaaa gacagctact aattaaccag
       61 gccaacgaat acatgaacag taagcaatgg cccggtaaag cggctattgg tcgtctgaaa
      121 ggtgacgaac tgacgcaata taatttgtgg ctggattatc tggacgcact ggagctggtc
      181 gatacttccg gtgcgccaga tattgaatgg cctacgcctc cggcagttca ggccagatga
      241 catccggcgc ggtgctggta tctgttgccg tcaccgcgtc aatgtaatcc agcacggcgt
      301 taagtcgggt tgtttctgcc tgagtcagtt tccgtccggc ctgtaacttc agctgaatca
      361 gactaatgga agccattgct gcatcaatca gtgattggcg ctgtgcttct gccgcgtcta
      421 ctgcggcact gtgttgtgcc tcagtgtctg tcacccattt ctcaccatcc catttatcgt
      481 atggcgttaa cggtgaaatc gtgacataac cgtctttgat ggcaccgata taatccactg
      541 taacagctgc gccattttcg attgagtaaa cagtctcatt gcgatggtct tcttcatggc
      601 tccatccctt acctgtaaat actgccactc ttcccggaat gttttcgcca gggtcaatac
      661 cagtggaaca ggcgggcata cttacgccag tattaatata ttcatcagac cagcccgtat
      721 actcagatgt ttcagcatca taataaaaac aacgcatatc gcccggcact gtagccagcc
      781 cattttcatc aaaaacaggt ttcattattt agccctcacc agaaagttaa atgcaatatt
      841 tcgcggtctg actgcaacaa aattcacacc atcacccaca gagttactgg tgaaattaaa
      901 tcgtgaaaat cctggctgat ttccggcgat gccatcatga aagttaattg cgtgtcccgc
      961 accttcgcct atattcccgg caaactgaga aaagtttgta gctgcctgcc agcttaataa
     1021 ttcgcgacca ccgtctgcac ctcgcccgtc atcccagata cgaatgaaat caccgcgggc
     1081 ttcaggtaat accagcgaag gaaacacttt cgccagcaca ggataatcag tggcagagaa
     1141 tttcgcgccg ttgaacttca aaaacaccat acaggaccag ctgtcgatta cagtatttgg
     1201 cattgcagcg gacggccaga agaacggaac gccaatagct ggagca
//
LOCUS       DABSYT020000333         1245 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.339, whole genome shotgun sequence.
ACCESSION   DABSYT020000333 DABSYT020000000
VERSION     DABSYT020000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.339"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            134..1234
                     /gene="ompC"
                     /locus_tag="ID725_RS26635"
                     /old_locus_tag="pgaptmp_005327"
     CDS             134..1234
                     /gene="ompC"
                     /locus_tag="ID725_RS26635"
                     /old_locus_tag="pgaptmp_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGV7853689.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          362 a          256 c          267 g          360 t
ORIGIN      
        1 atattatttc atctattttt ctcattgttt ttaatcacca ttctaattta aaaacagtat
       61 gtcatttata aatggcgccg tttaaaattc gtttaaaaaa tacgtttaac gataattaaa
      121 aggattctgt taaatgaaat taaaaatagt tgcggtggtt gtaactggtt tgttagctgc
      181 gaacgtagca cacgctgccg aagtctataa caaggatggt aataaactcg acctttatgg
      241 caaggttacc gctctacgtt attttactga tgataagcgt gacgatggtg ataaaactta
      301 tgcccgtctc ggctttaaag gagaaacgca aatcaatgat caaatgattg gttttggtca
      361 ctgggaatat gattttaaag gctataacga tgaagccaac ggctcgcgcg acaacaagac
      421 ccgtctggcc tatgctggtt taaaaattag tgaatttggc tctctggact atggccgtaa
      481 ctacggtgtc ggctatgaca ttggttcatg gactgatatg ttgccagaat ttggtggcga
      541 tacctggagt cagaaagatg tcttcatgac ataccgtacc accggtgtag caacctatcg
      601 caactacgat ttctttggct taattgaagg gctgaacttt gccgcgcaat atcaaggcaa
      661 aaatgaacgt actgacaaca gtcatcttta tggtgctgac tacacgcgtg ccaacggtga
      721 cggtttcggt atctcctcaa cttatgttta tgatggcttt ggtatcggag cggtgtatac
      781 caaatccgat cggacaaatg cgcaggaaag agccgctgct aatcctctca atgcctccgg
      841 taagaatgca gaactgtggg ctacaggtat aaaatatgat gccaacaaca tctactttgc
      901 agctaattac gctgaaacat taaacatgac cacctatggc gatggttata tttctaacaa
      961 agcacaaagt tttgaagtgg tggcgcaata tcaattcgac ttcggcttgc gcccctcact
     1021 cgcttacctg aaatcgaaag gcatagatct gggccgctac ggcgatcagg acatgattga
     1081 gtatatcgac gttggtgcga cgtatttctt caacaaaaat atgtcgacct atgttgatta
     1141 taaaatcaac ctgattgatg aaagcgactt tacccgtgcc gtagatattc gcaccgataa
     1201 catcgtcgct acgggcatta cctatcagtt ctaacatcct ctcct
//
LOCUS       DABSYT020000334         1243 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.340, whole genome shotgun sequence.
ACCESSION   DABSYT020000334 DABSYT020000000
VERSION     DABSYT020000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.340"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..519)
                     /locus_tag="ID725_RS26640"
                     /old_locus_tag="pgaptmp_005328"
     CDS             complement(<1..519)
                     /locus_tag="ID725_RS26640"
                     /old_locus_tag="pgaptmp_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGV7853690.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVEEIESNHNR"
     gene            complement(494..1060)
                     /locus_tag="ID725_RS26645"
                     /old_locus_tag="pgaptmp_005329"
     CDS             complement(494..1060)
                     /locus_tag="ID725_RS26645"
                     /old_locus_tag="pgaptmp_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGV7853691.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGAFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          299 a          296 c          236 g          412 t
ORIGIN      
        1 gcggttatgg tttgattcga tttcctctac tgcttcctgc ccgatgcggc taatcagttc
       61 gacgcgatac ggaacgagat ttccgctttt gtgctggttg cacaccacgc attgcttgtg
      121 aatattgcgt tcatcaaatc ggagttgagg tgccgcagca gttgtccggt aatgtccggc
      181 atcccactga gcagacgtga gcgttccgca cgagatacat ggtaagtcgc ggtctctttc
      241 tctgatgaag gcgtgtacgg cttgttgggc ttgtttaatc cagtaactgc ggggctttaa
      301 ggcgagtttt tgaatcttaa gtttatcttt ctgtttctgc tcctctcgtc gtcgtttctt
      361 ctctgctgcc ttttctgcct tttccgcttt ttcgcgttct ttacttcgtc gttcgagtgc
      421 tatcttggtt ccacaaatct cattacacca atattgattt tgatattttg gtataaacca
      481 ttcattgcaa catttacatt tccttcgata gattcgcata agtgctcctt tcgttgccgg
      541 aaaaatcacc gtaatactta tctcgggctt cttcagcaac tagtaccgct aactccagat
      601 catcaaagca tccgaagtgt ttactcttgc catggaatcc tagcctaaca ttccattttt
      661 tctgtcgttt gtgccaagta acccctctgc aacctgattt gctattcttt cggatcctta
      721 tatttcttga attttctatt ggcaggcatt ctcttaaatt ttctggccta ttgtcggtcc
      781 taattccatt aacgtggtca atttgaccag caggccaacg attatgagtt atgtaaaaaa
      841 ctaagacgtg agttttatat ctacgcccat ctatcatgat cattgaataa ccgttggaat
      901 caaaagctcc agcaacacta tttaatgcta tccttccctg agtgggaact ttccatctaa
      961 ataccccggt agatttatcg aaacttagta actcaaatat ccttttaaca gttaaatctt
     1021 ctcttttacg gttacatcgt cttcgcgctg gtttagccat cgccttcttc ctccgtaatg
     1081 gttttctgaa tttggccacc tgaacagagc tcaccaaagc tatggatgtc ggtatttcca
     1141 caataccaag atggcgaaaa taactgcatg ataagcctca gggaaaaggg aagacactac
     1201 ccccgataat tcagaaacaa atcgaaatac atgaactaaa gaa
//
LOCUS       DABSYT020000335         1241 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.341, whole genome shotgun sequence.
ACCESSION   DABSYT020000335 DABSYT020000000
VERSION     DABSYT020000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.341"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>1241
                     /locus_tag="ID725_RS26650"
                     /old_locus_tag="pgaptmp_005330"
     CDS             <1..>1241
                     /locus_tag="ID725_RS26650"
                     /old_locus_tag="pgaptmp_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGV7853692.1"
                     /translation="QRRALAELNSQLTEIRGSAVGMAGAFAGAFASGHLISLADEWSS
                     VNARLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVL
                     KVTEAISTGLKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAG
                     MGVARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAW
                     VGGANEASGVTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGL
                     VTAARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQER
                     LNRNIAARTAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQAR
                     ESARQYALTIDEIAHKTPSMS"
BASE COUNT          269 a          304 c          421 g          247 t
ORIGIN      
        1 agcagcgacg tgccctggca gaactgaaca gccagttgac ggaaattcgc ggttctgctg
       61 tcggaatggc tggcgcattt gccggtgcct ttgcctccgg acacctgatt tcactggcgg
      121 atgagtggag ttccgtaaat gcccgtctga aacaggcatc acagtcatcc gatgaatttt
      181 cgtcatcaca gaaagtgctg atggatatca gccagcggac aggcacggca ttttcggata
      241 atgcggccct gtttgcccgt tcggctgcct cgatgcgtga atatggttac agtgccggtg
      301 atgtactgaa ggtgacggag gccatttcca cggggctgaa aatctccggt gccagtacgg
      361 ctgaggcggg ttcggtgatc acccagttca gccaggcgct ggcacagggt gtgttgcgcg
      421 gtgaggaatt taattcggtc aatgaaagtg gtgaccggat cgtacgtgca ctggctgcgg
      481 gtatgggcgt ggcccgtaaa gatctgaagg caatggcgga tgacggaaaa ctgacagcgg
      541 ataaagtggt ccccgcgtta atcagccagc tggggatatt acgtgatgaa tatgcggcca
      601 tgccggaaac ggtttccagt agtatcacga aggtggaaaa cgcctttatg gcctgggtgg
      661 gcggtgcgaa tgaggccagc ggggtgacaa aaacgctctc cggcatgctg aacggtgttg
      721 ccggacagat tgataatgtg gcaacagccg tgggcgcgct ggttgccgtc ggggttgcac
      781 gttactttgg caatatggcc tccggagcga tgtctgccac ggcaggactt gtgacggctg
      841 cacgtaatga agttgcactg gcggaagcac agttcagggg aacgcagatt gccacggcgc
      901 gggcaagggc agccgtgtac cgtgctcagc aggccgtggc ggcagcccgc gggacggaga
      961 tgcagattgc agcagaggcc cgtctggcgg ccacacagga acgcctgaac agaaatattg
     1021 ctgccagaac cgccgcccag aatgcgctga acagtacaac ggcggtgggc tcacgtctga
     1081 tgagcggtgc gctggggctg gttggtggcg tacccggact ggtgatgctg ggggctgcag
     1141 catggtacac gctgtaccag aatcaggagc aggccaggga gtctgcgcgc cagtatgcac
     1201 tgacgataga tgaaatcgcg cataaaacgc cgtcaatgtc t
//
LOCUS       DABSYT020000336         1241 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.342, whole genome shotgun sequence.
ACCESSION   DABSYT020000336 DABSYT020000000
VERSION     DABSYT020000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.342"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..303
                     /locus_tag="ID725_RS26655"
                     /old_locus_tag="pgaptmp_005331"
     CDS             <1..303
                     /locus_tag="ID725_RS26655"
                     /old_locus_tag="pgaptmp_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HGV7853693.1"
                     /translation="FTGLSLAISHRHDHVVKLYGKLFKKLDTSPYKMSIILALAIDCE
                     RNNANIIIDSEYKSNKAVKEYVEILKEFNICPEKVAEYLSEFSGKHFLDVYNYYSN"
     gene            485..670
                     /locus_tag="ID725_RS26660"
                     /old_locus_tag="pgaptmp_005332"
     CDS             485..670
                     /locus_tag="ID725_RS26660"
                     /old_locus_tag="pgaptmp_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGV7853694.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRIIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            911..>1241
                     /gene="nleB"
                     /locus_tag="ID725_RS26665"
                     /old_locus_tag="pgaptmp_005333"
     CDS             911..>1241
                     /gene="nleB"
                     /locus_tag="ID725_RS26665"
                     /old_locus_tag="pgaptmp_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HGV7853695.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENE"
BASE COUNT          444 a          200 c          223 g          374 t
ORIGIN      
        1 tttacggggt tatcattagc gatcagtcat agacatgatc atgttgtcaa attatacgga
       61 aagttattta aaaaacttga taccagtcca tataaaatgt caattatcct agcattagca
      121 attgattgcg agagaaataa tgcaaacata attattgata gtgagtacaa gtcaaataag
      181 gcagtaaagg aatatgtaga aattttaaaa gaattcaata tatgcccaga aaaggtagca
      241 gaatacctaa gcgagttcag cggaaaacat ttcctcgatg tatataatta ttattccaat
      301 tgaaaaaaat ataaagtatc tatcacggca ctctcagttg agtatcacag gcacatgtat
      361 tttagccccc tgagtctaca gacaaccaat attacttaaa taagtgataa tcttaatgct
      421 gttttttaga acagatgatt gatatttttg ggccggagaa acgcagacag gctttgttga
      481 ataaatcgag cttttgctga gtttaaggat cagatcactc attttcctgc aaaagctcat
      541 tgggcgaaac aatctgactc agagaacccg gattaaacgt ctggcccgca gaatcatcta
      601 tttctcacgc actgtagtac gtaataaaaa agtcatcggt acttttatca aaaaacatat
      661 attctattaa ttattgccat cacctttttc ttattgacaa cattactctg ctgattgtta
      721 aaatctaacc tgtattcagg gttacgattc tatacaatag aacagggagg ggaaaggatg
      781 tcatcgttaa tgagactttt tgttaatgtc aacaatgcta tgaatttatt ttagaaaaac
      841 aattaagctg acgtagtgtt ccgtctaaat gacggggcga tattaacatg attagaacaa
      901 gaggaatttt atgttatctt cattaaatgt ccttcaatcc agcttcagag gaaagacagc
      961 tttatcaaat agtacacttc tccagaaagt ttcttttgct ggaaaagaat atcctctgga
     1021 acctattgat gaaaaaaccc ctattctttt tcagtggttt gaagcaaggc cagagcgata
     1081 cgaaaaagga gaagtaccaa tattgaatac caaagaacat ccgtatttga gcaatattat
     1141 aaatgctgca aaaatagaaa atgagcgtat aatcggtgtg ctggtagatg gaaattttac
     1201 ttatgaacaa aaaaaggaat ttctcagtct tgaaaatgaa t
//
LOCUS       DABSYT020000337         1239 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.343, whole genome shotgun sequence.
ACCESSION   DABSYT020000337 DABSYT020000000
VERSION     DABSYT020000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.343"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..441
                     /locus_tag="ID725_RS26670"
                     /old_locus_tag="pgaptmp_005334"
     CDS             <1..441
                     /locus_tag="ID725_RS26670"
                     /old_locus_tag="pgaptmp_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGV7853696.1"
                     /translation="DEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCD
                     QVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            597..779
                     /locus_tag="ID725_RS26675"
                     /old_locus_tag="pgaptmp_005335"
     CDS             597..779
                     /locus_tag="ID725_RS26675"
                     /old_locus_tag="pgaptmp_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853697.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            792..923
                     /locus_tag="ID725_RS26680"
                     /old_locus_tag="pgaptmp_005336"
     CDS             792..923
                     /locus_tag="ID725_RS26680"
                     /old_locus_tag="pgaptmp_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853698.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            931..1170
                     /locus_tag="ID725_RS26685"
                     /old_locus_tag="pgaptmp_005337"
                     /pseudo
     CDS             931..1170
                     /locus_tag="ID725_RS26685"
                     /old_locus_tag="pgaptmp_005337"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            1151..>1239
                     /locus_tag="ID725_RS26690"
                     /old_locus_tag="pgaptmp_005338"
     CDS             1151..>1239
                     /locus_tag="ID725_RS26690"
                     /old_locus_tag="pgaptmp_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853699.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAP"
BASE COUNT          307 a          263 c          367 g          302 t
ORIGIN      
        1 gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat ctggaccatc
       61 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      121 gaaaaatgcg accaggttaa cgccattgaa cgtgataagg cgctggagtg ggtggagcgc
      181 aatattaaag taccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
      241 aacattggtc ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat
      301 cgcaggggag cgtgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      361 attcgctcaa acaactgcta cggtcaggtt tcacggcgtg accaggagag cgcgctggcg
      421 tgctggggaa ttgacagata agcagaatat tttgctgaaa aatgcggttt gctcacactg
      481 gcggataaca cgaaatcctg cgaactggca aaaactaagt gaataaaagt aaaaccccgt
      541 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      601 gaaagtataa acgaattctg ttgagattga ctatgaaaaa cggccttgaa ctgaaagcgc
      661 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
      721 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      781 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
      841 gcatttcatt ttcactaatg ctggttgcta tttgcttttc cactgcctgg cgcgtcttaa
      901 ctttgtcatg gctggtgggg tgagggggat atgaaccgtg ttctgtgtgt ggtgattatt
      961 gtcctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg cgataacgcc
     1021 attacctaca aagcgcaacg cgataaaaaa gccagtgagc tgaagctggc gaacgtgaca
     1081 attactgata tgcaggtacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1141 gaattagccg atgcgagagc tgaaaatgaa actctgcgtg ctgatgttgc cgctggtcgt
     1201 aagcgcctgc gcatcaacgc caactgtcca ggctccttg
//
LOCUS       DABSYT020000338         1237 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.344, whole genome shotgun sequence.
ACCESSION   DABSYT020000338 DABSYT020000000
VERSION     DABSYT020000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.344"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..103
                     /locus_tag="ID725_RS26695"
                     /old_locus_tag="pgaptmp_005339"
     CDS             <1..103
                     /locus_tag="ID725_RS26695"
                     /old_locus_tag="pgaptmp_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853700.1"
                     /translation="CSCYFTTGSVDEEKSKSDKIDFTFSGAATTADL"
     gene            711..>1237
                     /locus_tag="ID725_RS26700"
                     /old_locus_tag="pgaptmp_005340"
     CDS             711..>1237
                     /locus_tag="ID725_RS26700"
                     /old_locus_tag="pgaptmp_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853701.1"
                     /translation="MNSYIKIDQTGSNKFKITDLYREFNGETKCYDKKCRDMDFSGRN
                     VKEFIRLLSDHDQFEKDQISELTVAANALKLEVAKNNYNMKYSFDTQTERRMIELIRE
                     QKDLIPEKYLHQSGIKKLKLHEDEFSSLLVDAERQVLEGSSFVLCCGEKINSTISELL
                     SKKITDLTHPTESFTL"
BASE COUNT          441 a          173 c          235 g          388 t
ORIGIN      
        1 gtgctcttgt tattttacta ccggtagtgt cgacgaagaa aaatccaaaa gcgataaaat
       61 cgattttaca ttttccggtg ccgcaacaac tgctgattta tgattttttc ttgataaagc
      121 gcgtttatca ttaaggtgaa cacgtattgt taaccgtctg tgtgtaaaca gtctttttga
      181 aaaaatgagc gatttttgat agtgaataag tagtattagt gatgcaattg cattggggaa
      241 atcgataaaa attaacggca ctatagtctt attattgaaa taaatgaaat atagcggtta
      301 gcattgagat atttatcatg ctagccattg atacttctaa caatgtcaaa atatgcaaag
      361 tgtcatattg cgaggttact atggttagtg tgacgagtta ttcatatcca acggaaacag
      421 agaatgataa tagccagttt caaaatcaga gtctaaggga gagtatcgac aaaagtctcg
      481 aggataatat ttataaattt aatatgatag aacaaagcgg cctgctcgat aattataggg
      541 caaggtttaa agatgacact tttcgtgaca tttttcgaag ttttttatta tatcttttag
      601 attcatttca tatctatcgg catgatatta gtgataaatt ggttccttat tttcatgtta
      661 cgcctgaatc ggagaatgcg aattataaga catagattgc cgttacaggc atgaatagct
      721 atataaaaat tgatcagacc ggaagtaata aatttaaaat cactgatctg tatcgtgagt
      781 tcaatggcga aacaaagtgt tatgataaaa agtgtaggga tatggatttt agtggtcgaa
      841 atgtaaaaga gtttataagg ttattatcag atcacgatca atttgaaaaa gatcaaatct
      901 cagaattaac agttgctgca aatgctctta agctggaagt tgctaaaaat aactataaca
      961 tgaaatactc ttttgatacg caaaccgaac gccgaatgat agagcttata agggaacaaa
     1021 aagatttaat tccagaaaaa tatttacatc aatcaggtat aaaaaaactt aagcttcatg
     1081 aggatgaatt tagctcactc cttgtagatg cagaaaggca agttctggag ggaagttcat
     1141 ttgttttatg ttgcggtgaa aaaataaaca gcactataag tgaattactt agcaaaaaaa
     1201 ttacagattt aacacatccc accgaatctt tcactct
//
LOCUS       DABSYT020000339         1190 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.345, whole genome shotgun sequence.
ACCESSION   DABSYT020000339 DABSYT020000000
VERSION     DABSYT020000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.345"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..1085
                     /gene="ehxD"
                     /locus_tag="ID725_RS26705"
                     /old_locus_tag="pgaptmp_005341"
     CDS             <1..1085
                     /gene="ehxD"
                     /locus_tag="ID725_RS26705"
                     /old_locus_tag="pgaptmp_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HGV7853702.1"
                     /translation="KNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESIQRN
                     ELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQITLNKKIVERDAAL
                     ARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQVSQL
                     EKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAPVSG
                     TVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVDAYP
                     YTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVMAEI
                     KTGVRSVISYLLSPLKETINESLRER"
BASE COUNT          430 a          173 c          247 g          340 t
ORIGIN      
        1 aaaaaaatga tccattagtt gaattgacgg tgcctggtgt tgaatctgat attttaaaat
       61 cagaagcatc tttgttgtat gaaaaaacag aacaatatcg ctacgcaatt ctttctgaat
      121 caatacaacg gaatgagctt cctgaaataa ggataacaga ttttcctggc ggagaagata
      181 atgcgggagg tgaacatttt cagagggtta gctcattaat aaaagagcaa ttcatgacat
      241 ggcagaacag aaaaaatcaa aagcagatca cattaaataa aaaaatagtt gaacgggatg
      301 cagcgcttgc tcgtgttagc ctgtatgagc atcaggtatc acaggaagga agaaaactca
      361 atgattttaa gtatttgttg aataaaaaag ctgtttctca acattcagtt atggagcagg
      421 aaaatagcta tattcaggca aaaaatgaac atgcagtctg gcttgcacag gtttctcaac
      481 ttgaaaaaga aatagaactt gtgcgggaag aactggcact ggagacgaat atcttcagaa
      541 gtgaaattat cgagaagcac agaaaatcaa cagataacat tgtgttgctg gagcatgagc
      601 ttgaaaaaaa caggcagaga aaagcatcat cttttattaa agctcctgtg agtggtactg
      661 ttcaggagtt aaatatacat acagaaggtg gtgttgtcac aacagcagaa acgctgatga
      721 ttattgtccc tgataatgat attctcgaag taacagcatc tgtacttaac aaggatatcg
      781 gttttataca acctggacag gaggtcgtta ttaaagtaga tgcatatcca tatacacgtc
      841 atggttacct tacagggaaa gtaaaaaata taactgcaga ttctgtttct gttccggata
      901 cagggcttgt atttaacgtg attatatctg ttgatcggaa tgatatacag ggagaaagaa
      961 aaaaaatccc tgttacagct ggaatgactg ttatggcaga aataaaaacc ggggtgcgta
     1021 gtgttatcag ctatcttctt agtccattaa aggaaactat taatgaaagt ttacgtgaac
     1081 gttagtatat aaataacata ttggcttttt atatgatata gatttctgaa ataatcttgt
     1141 aaagattatc tatgttattt aaacaaacta tgatacagtt tttatcttta
//
LOCUS       DABSYT020000340         1182 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.346, whole genome shotgun sequence.
ACCESSION   DABSYT020000340 DABSYT020000000
VERSION     DABSYT020000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.346"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(1..>1182)
                     /locus_tag="ID725_RS26710"
                     /old_locus_tag="pgaptmp_005342"
     CDS             complement(1..>1182)
                     /locus_tag="ID725_RS26710"
                     /old_locus_tag="pgaptmp_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGV7853703.1"
                     /translation="RRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALI
                     SEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGR
                     YITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSG
                     DLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSG
                     VSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWV
                     N"
BASE COUNT          257 a          382 c          247 g          296 t
ORIGIN      
        1 ttaattgacc cagcattctt gccacgttct acaagccaga gtcataactg aagcgtcaac
       61 acctgcaccg ataccaacag attctgtcga tgattctgga ttatcaacat ccactacata
      121 agtatacgca aaatgccccc caactccgcc gccaaccgtt acacctactg aattgctgac
      181 tcccgatgta ggtaaagact tggtctcaga atatgtgata gccgcattta tcccaccacc
      241 aattcctgca tgtccgcatg ctgtagcata aatacaaata tcagataaca tttcaccttt
      301 ctttttagca aacgcaacgc caagtcctgc atgaccacct aatgatatta gatctccact
      361 gccagtaata tcaattgatg atgttccata tgaatcatca tgccaaacat catataataa
      421 ttgatataaa ttatacaaac ccaaaggatc catccttatt atcggattca gtgggtaatt
      481 atacagattc catccccccg ccagcccaat ggggtcttgt gtaatatacc tcccctgtag
      541 cgggtcatag taccggttcc ggttatagta cagccctgat tcctcatcat gctgctgccc
      601 cggcagacgg tatggctggt gcaggtaata cgggttctct tcattaagct ggttgcccca
      661 ttcatcatac tccccgcgcc acgccgtatt gccgtcttcg ctgatgagcg ccagcggcag
      721 gccccggtgg tcgcagtggt aaaaatgaac ttttcgcgcc ggtgtgtatt ccggctccac
      781 ctgtctggcc agttgctcca ccgtcagccc gcactgcgca agccacgccc ggctttcact
      841 gctcacgcgg tctgcccgga tttcttcctc cagcctgtcc agcagccgca ccagttcagc
      901 cgggaacacc acgccgtggc cgttctcact cccttcctgc tggagcgtct ctgccaggct
      961 gcgccgctgc gctttttccc gctcgccgtt ctctgtctcg acccggatga gcggcgtgaa
     1021 gcttcccggc tcgtataccg tctggatacg tgtggtgtca gtctgcaccg tcgtcagcct
     1081 gtctccgtcc cagccatacc acgtcacctc cggtttacgc gacagcgaca tccaccccgt
     1141 caggtcacgc tcccgccgcc agacccgttt tgccattcgc cg
//
LOCUS       DABSYT020000341         1180 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.347, whole genome shotgun sequence.
ACCESSION   DABSYT020000341 DABSYT020000000
VERSION     DABSYT020000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.347"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..253
                     /locus_tag="ID725_RS26715"
                     /old_locus_tag="pgaptmp_005343"
     CDS             <1..253
                     /locus_tag="ID725_RS26715"
                     /old_locus_tag="pgaptmp_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGV7853704.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            268..801
                     /locus_tag="ID725_RS26720"
                     /old_locus_tag="pgaptmp_005344"
     CDS             268..801
                     /locus_tag="ID725_RS26720"
                     /old_locus_tag="pgaptmp_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853705.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            798..>1180
                     /locus_tag="ID725_RS26725"
                     /old_locus_tag="pgaptmp_005345"
     CDS             798..>1180
                     /locus_tag="ID725_RS26725"
                     /old_locus_tag="pgaptmp_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGV7853706.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          280 a          274 c          359 g          267 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggtgacacgc tgaccattaa cggcgagatg ttctgggtgg accgtgtttc
      181 tccggatgac ggagggagtt gttatctctg gctcaaccgt gggcaaccac cggcagttaa
      241 ccggcgacga taaacgcagg gtgaattatg gcgataaaag ggcttgatca ggcgattgaa
      301 aatctgagcc gggttcgtaa aaacgccatt ccggcggctt cagcaatggc cattaaccgc
      361 gtggccacaa cggcgattaa tcagtcttca tcacaggttg cccgggagac aaaggtacgc
      421 cggaaactgg taaaggaacg gtccagactg aaacgggcca cggtcagaaa tccgaatgcc
      481 agaattatcg ttaaacgcgg tgatctccct gtgattaagc tggggatcag gatgctgggc
      541 cgtcgtccgg acagcatact taaagctggt cagcatcggt atcagcgggc atttatccag
      601 cgattaaaaa atggtcgctg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc
      661 attgatgtgg tgaaaatccc gatggcggcc ccactgaaac aggcgtttga tgagaatatt
      721 gaccgtatcc ggcgtgaacg tctgcccgga gaactggcat acgcgctgaa acaacaactt
      781 aggattgcga taaaacgatg aaacatactg atatccgtgc ggcagtgctg gatgcactgg
      841 agctgcatga acacggggcg acgctgtttg atggtcgccc cgttgttttt gacgaagagg
      901 attttccggc cgtcgcggtt tatctgacgg atgcagagta taccggtgaa gagctggatg
      961 cagatacctg gcgggccaca ctgcatattg aggtgttttt accggcacag gtaccggatt
     1021 cggagcttga tcagtggatg gaaagccgga tttatccggc ggtgactgcg atcccggcac
     1081 tggcagacct gattacgacg atggttacgc agggctatga gtatcgtcgt gatgacgata
     1141 tggcgttatg gagttctgcg gatctgactt attccattac
//
LOCUS       DABSYT020000342         1159 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.348, whole genome shotgun sequence.
ACCESSION   DABSYT020000342 DABSYT020000000
VERSION     DABSYT020000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.348"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..90
                     /locus_tag="ID725_RS26730"
                     /old_locus_tag="pgaptmp_005346"
     CDS             <1..90
                     /locus_tag="ID725_RS26730"
                     /old_locus_tag="pgaptmp_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853707.1"
                     /translation="LLRQTQDNQFSTLGKKNGHMKSIDNKSSL"
     gene            163..732
                     /locus_tag="ID725_RS26735"
                     /old_locus_tag="pgaptmp_005347"
     CDS             163..732
                     /locus_tag="ID725_RS26735"
                     /old_locus_tag="pgaptmp_005347"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HGV7853708.1"
                     /translation="MTSRDQGINSELSNRTIQFKEKIHNGIHTEYITDQKHSNNKDRE
                     KKYRDGDKINGPQAHSLDITNERRFADNRTMFTQHIEKQRNVNTLNQNDINNSANNAN
                     VRENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSDRLHEAQHNDVTAQRWVLTE
                     QDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            692..>1159
                     /locus_tag="ID725_RS26740"
                     /old_locus_tag="pgaptmp_005348"
     CDS             692..>1159
                     /locus_tag="ID725_RS26740"
                     /old_locus_tag="pgaptmp_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HGV7853709.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPT"
BASE COUNT          420 a          211 c          243 g          285 t
ORIGIN      
        1 ttattacgtc agactcagga taaccaattt tctacgttgg ggaaaaagaa tgggcacatg
       61 aagtctattg acaacaaatc gtctctctag gttgacagga atccatctct ttctttgcaa
      121 acaaaaggag ccgagcaatt ttctacaaag aaacttctca atatgacttc acgagatcag
      181 ggtattaatt ctgaactatc taatcgaacg atacaattta aggaaaaaat ccataatggt
      241 atccatacag aatacatcac agatcaaaaa cacagtaata ataaagatcg tgaaaaaaaa
      301 taccgcgatg gagacaaaat aaatggaccg caggcccatt cattagacat tactaacgaa
      361 agaaggtttg ctgataacag aaccatgttt actcaacata tcgaaaagca aaggaatgtt
      421 aatacgctca atcagaatga tataaataat tctgctaata atgccaatgt gcgcgaaaat
      481 gaattgacat atcagtttca gcgttgggga caaaatcata cggtccggat tctggaaagt
      541 agtgaaggga tacgactaaa accatcagac acacttgtct ccgacagact ccatgaagcc
      601 caacacaatg atgttacagc acaacgttgg gtattgactg agcaagatga acgacaagga
      661 caacgacatc agcctcatga ggagcaagaa aatgaaggca aattcgaaaa cgatcagaag
      721 gatgaaagtt aatgttcggt ctgaggaaag taaatcgaaa cactctacgt ttgaaaccac
      781 ttttcaaaat tggaaagaaa atggcgaaga tgttgcatta ttaatgcccg aatttagtgc
      841 taaatggcta ccgatagcag aagagagtgg cagctggtct ggatgggtgt tgcttcgaga
      901 aatatttccc ttaatttctg ccgagctcgc tgggatggct ttaatgccag aaacggaaag
      961 gttaataggg gaatggctca gtttatccag ttccccgtta aatctaaagt atcctgaact
     1021 aaaatataat cgtttgtgcg taggtaaagt atttgatgga gtattgagtc cagctcaacc
     1081 actgataaga atatggacag gggaattgaa tctttggtta gataaagtca cagtctgcca
     1141 atacgaaaac gctccaact
//
LOCUS       DABSYT020000343         1156 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.349, whole genome shotgun sequence.
ACCESSION   DABSYT020000343 DABSYT020000000
VERSION     DABSYT020000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.349"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>1156
                     /locus_tag="ID725_RS26745"
                     /old_locus_tag="pgaptmp_005349"
     CDS             join(<1..274,274..>1156)
                     /locus_tag="ID725_RS26745"
                     /old_locus_tag="pgaptmp_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611570.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HGV7853710.1"
                     /translation="RFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTPETLRV
                     WVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFCEGGVRPPLE
                     KMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKREIQR
                     VYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVA
                     AGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTF
                     VLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAE
                     SINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAE"
     misc_feature    267..383
                     /locus_tag="ID725_RS26745"
                     /old_locus_tag="pgaptmp_005349"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          274 a          276 c          358 g          248 t
ORIGIN      
        1 cgtttttccc ccgaagtccg tcagcgggcg attcgtatgg ttctggaaag tcaggatgaa
       61 tatgactcac agtgggcggc aatttgttcc attgccccaa agattggctg tacgccggag
      121 actctgcgtg tctgggttcg ccagcatgag cgggataccg ggggcggtga tggtgggctc
      181 accagcgctg aacgtcagcg tctgaaagag ctggaacgtg aaaatcgtga actgcgccgc
      241 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
      301 tggaaaaaat gatgccactg ctggataagc tgcgtgagca gtacggggtc ggaccggtat
      361 gcagcgaact gcatattgcc ccgtcaacgt attaccattg tcagcaacag cgacatcatc
      421 cggataaacg cagtgcccgt gcgcagcacg acgactggct gaagagagag atacagcgcg
      481 tatacgatga aaatcatcag gtgtacggtg tgcgtaaagt ctggcgtcag ttgttacggg
      541 aaggaatcag ggtggccaga tgtacagtgg cacgtctcat ggcggttatg ggacttgccg
      601 gtgttctccg gggtaaaaag gtccgtacga ccatcagccg gaaagccgtt gccgcaggcg
      661 accgcgtaaa ccgtcagttc gtggcagaac gacctgacca gctgtgggtg gctgatttta
      721 cttacgtcag cacatggcag ggcttcgtct atgtggcgtt tatcattgat gtgtttgccg
      781 gatacatcgt ggggtggcgg gtctcatcgt ctatggaaac gacattcgtg ctggatgcgc
      841 tggagcaggc gttgtgggcc cgtcgtccgt ctggcaccat ccatcacagc gataaaggct
      901 ctcagtatgt gtcactggcc tatacggagc gactaaaaga agccggatta ctggcatcaa
      961 cagggagtac aggcgactcg tatgacaacg cgatggctga gagcatcaat ggtctttaca
     1021 aagcggaggt aatacaccgt aagagctgga aaaaccgtgc agaagtggaa ctggccacac
     1081 taacgtgggt ggactggtat aacaatcgac gattgctggg aaggctgggc catactcctc
     1141 cggcagaagc agaaaa
//
LOCUS       DABSYT020000344         1139 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.350, whole genome shotgun sequence.
ACCESSION   DABSYT020000344 DABSYT020000000
VERSION     DABSYT020000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.350"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..370)
                     /locus_tag="ID725_RS26750"
                     /old_locus_tag="pgaptmp_005350"
     CDS             complement(<1..370)
                     /locus_tag="ID725_RS26750"
                     /old_locus_tag="pgaptmp_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HGV7853711.1"
                     /translation="MNNTLLPSINIPCTLFETISLFDDYSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFGQYKTLIEDLIEHFRYGNG"
     gene            557..703
                     /locus_tag="ID725_RS26755"
                     /old_locus_tag="pgaptmp_005351"
                     /pseudo
     CDS             557..703
                     /locus_tag="ID725_RS26755"
                     /old_locus_tag="pgaptmp_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          373 a          188 c          204 g          374 t
ORIGIN      
        1 tgccatttcc atatctaaaa tgttcaatca gatcttcgat aagtgtttta tattgtccaa
       61 aaaaggaaaa catttttgct agttctttca tttctgcaaa taaaatatca gtgcattcac
      121 gttgtgatat ttttgttccg gaacttgagg cactaaaagc aacattaata gaacctgggc
      181 cagggaaatg gtatttaata agtctgtaag gatcaacttt tgctgagata tcgcttaggc
      241 cgagtgagag aaaatcttgt tctaccatat caccatattg catatcatca gcagaataat
      301 catcaaataa actaatagtt tcgaaaagtg tacagggaat attaattgat ggaagtaatg
      361 tgttattcat ttacattttc cttggttata tttaagctca atatcaattt gagaacaaat
      421 gctagggtaa aaaaatattt aaaaaagaag tacacttcac attttttcat tacatctctg
      481 aagagaacgt cgttaatgat tggtcttgct cattgttaat aacaaaatat tgaatttacc
      541 gattaggaaa ctttttccta cgccgattgc ctccagctat ggcatgaact ttgagtttat
      601 gccagaactg aagtggagtt tcggctaccc aggcgcgatt atctttatga tcctcgcggg
      661 cctggcaccg tatctgtact ttaagcgcaa gaactggctg taactatacg atgtcgctgt
      721 gagtaagtta gtgtgctgac agcgacattt tagcagaggg aatcgcagat aacataattg
      781 ttgtgaataa tatccatgcc acgttgagtt ggacgcttaa tgactcacat ctcacccctt
      841 caattgctgc agagataata gaaaaaagtg tcaaacccaa tgaaatcaat acaaagtaga
      901 ttgccttaca tccagtatct cttattcttt ttgctccaat attgatattt acataggtat
      961 tattaataac cagaataaaa ctcaaagcca ggttgatatc attcaaagtt attaatattg
     1021 accatgatgt taatgtatag attatgaact gaaagcgtga taaacgctca agcttggatg
     1081 ggtagaatat atttctgatg gatgatttga acatttttta cattgtttta tatatataa
//
LOCUS       DABSYT020000345         1137 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.351, whole genome shotgun sequence.
ACCESSION   DABSYT020000345 DABSYT020000000
VERSION     DABSYT020000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.351"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>1137
                     /locus_tag="ID725_RS26760"
                     /old_locus_tag="pgaptmp_005352"
     rRNA            <1..>1137
                     /locus_tag="ID725_RS26760"
                     /old_locus_tag="pgaptmp_005352"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          284 a          268 c          361 g          224 t
ORIGIN      
        1 agctaatacc gcataacgtc gcaagaccaa agagggggac cttcgggcct cttgccatcg
       61 gatgtgccca gatgggatta gctagtaggt ggggtaacgg ctcacctagg cgacgatccc
      121 tagctggtct gagaggatga ccagccacac tggaactgag acacggtcca gactcctacg
      181 ggaggcagca gtggggaata ttgcacaatg ggcgcaagcc tgatgcagcc atgccgcgtg
      241 tatgaagaag gccttcgggt tgtaaagtac tttcagcggg gaggaaggga gtaaagttaa
      301 tacctttgct cattgacgtt acccgcagaa gaagcaccgg ctaactccgt gccagcagcc
      361 gcggtaatac ggagggtgca agcgttaatc ggaattactg ggcgtaaagc gcacgcaggc
      421 ggtttgttaa gtcagatgtg aaatccccgg gctcaacctg ggaactgcat ctgatactgg
      481 caagcttgag tctcgtagag gggggtagaa ttccaggtgt agcggtgaaa tgcgtagaga
      541 tctggaggaa taccggtggc gaaggcggcc ccctggacga agactgacgc tcaggtgcga
      601 aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgtcgac
      661 ttggaggttg tgcccttgag gcgtggcttc cggagctaac gcgttaagtc gaccgcctgg
      721 ggagtacggc cgcaaggtta aaactcaaat gaattgacgg gggcccgcac aagcggtgga
      781 gcatgtggtt taattcgatg caacgcgaag aaccttacct ggtcttgaca tccacagaac
      841 tttccagaga tggattggtg ccttcgggaa ctgtgagaca ggtgctgcat ggctgtcgtc
      901 agctcgtgtt gtgaaatgtt gggttaagtc ccgcaacgag cgcaaccctt atcctttgtt
      961 gccagcggtc cggccgggaa ctcaaaggag actgccagtg ataaactgga ggaaggtggg
     1021 gatgacgtca agtcatcatg gcccttacga ccagggctac acacgtgcta caatggcgca
     1081 tacaaagaga agcgacctcg cgagagcaag cggacctcat aaagtgcgtc gtagtcc
//
LOCUS       DABSYT020000346         1117 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.352, whole genome shotgun sequence.
ACCESSION   DABSYT020000346 DABSYT020000000
VERSION     DABSYT020000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.352"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..128)
                     /locus_tag="ID725_RS26765"
                     /old_locus_tag="pgaptmp_005353"
     CDS             complement(<1..128)
                     /locus_tag="ID725_RS26765"
                     /old_locus_tag="pgaptmp_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGV7853712.1"
                     /translation="MHRDSERSRRIRGWVIMKKDLKTLALARLSGFRHKTVKVPEWG"
     gene            complement(95..811)
                     /locus_tag="ID725_RS26770"
                     /old_locus_tag="pgaptmp_005354"
     CDS             complement(95..811)
                     /locus_tag="ID725_RS26770"
                     /old_locus_tag="pgaptmp_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGV7853713.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(877..>1117)
                     /locus_tag="ID725_RS26775"
                     /old_locus_tag="pgaptmp_005355"
     CDS             complement(877..>1117)
                     /locus_tag="ID725_RS26775"
                     /old_locus_tag="pgaptmp_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HGV7853714.1"
                     /translation="WVVFSLISSVSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDA
                     REAIMLLAPGSVSEMQDYIPENRCYRATLEFQVTV"
BASE COUNT          253 a          343 c          267 g          254 t
ORIGIN      
        1 ccccattccg gcaccttcac cgttttatga cgaaaccctg acagtctggc cagcgccagc
       61 gttttcagat cctttttcat gatgacccat ccccttatcc ggcggctgcg ctcactgtca
      121 cggtgcattc aacagacgtc acactctgtg ctttctctgc cgaatcggtc accacacagg
      181 tatatttccc cgcatcagcg gactgcgcac ctggcttact gaaggtgtct gtcgtctgcc
      241 cgtcaaccgg ctgaccatcc ttcttccagg cgtatttata cggcggcgtt cccccgttgg
      301 cactgactga cattgtcagc agcgcaccgg tattcacggt aagtgtctta tccagatttt
      361 tcacaaacgc cagcggtacc acaaaggaca ccggtttgcc tttcagacgc agtgagaacg
      421 ttgcagccac cacgccgttg gtaccggatg accaggtgtg ctggcgcact tccgccagga
      481 atttaaagcc cttaccggac ggaaacagca ccttaaacgc atacaacgcg tcattgtcat
      541 aggcatcacg cagggcgttc tgggcctgat tcagataaaa attgcccgac atggaaatct
      601 cagacgacgc ccccagacca ttgatgttct cctgctctgt ggagcagagc gtggtcacat
      661 caatatcctg tttctgcccg gcggtgaact gcacttcctt gatggtgcag tccaggcgca
      721 gatattccgc cttatccata gtttcagcag tcgccggggc agatgaaatc atcacctgcg
      781 tcagctgtga gcgttcatac aaagcagaca ttctgcctcc tgataataaa aaacccgcac
      841 gcggcggggt atgggttttg tagaaaaaag aaaaagtcac accgtgacct gaaactccag
      901 ggttgcacgg taacagcggt tttccggaat atagtcctgc atttcactga cggatcccgg
      961 ggccagcagc attatggctt cacgggcgtc ctgacgtatc tgacgcgcct gcgtcacagt
     1021 cccggcataa acgtctatct gcaccgacac tgaggactcc gcctgcccgc ccatcacgtc
     1081 cgctgacacc gatgaaatca ggctgaaaac cacccac
//
LOCUS       DABSYT020000347         1092 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.353, whole genome shotgun sequence.
ACCESSION   DABSYT020000347 DABSYT020000000
VERSION     DABSYT020000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.353"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            80..>1092
                     /locus_tag="ID725_RS26780"
                     /old_locus_tag="pgaptmp_005356"
     CDS             80..>1092
                     /locus_tag="ID725_RS26780"
                     /old_locus_tag="pgaptmp_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGV7853715.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHR"
BASE COUNT          368 a          169 c          238 g          317 t
ORIGIN      
        1 tgtcttttta aataaaaatt aaacaaaaat tatatcccac cactaaggtt tataaaagca
       61 tacgttagca ggtgtcacca tgaaaaaagc catagcatat atgcgatttt catcaccagg
      121 tcagatgtct ggcgactcat taaaccgaca gagaagactt attgctgaat ggttaaaggt
      181 aaatagtgat tattatcttg ataccataac atatgaagat ttaggattaa gtgcattcaa
      241 aggaaagcat gcacaatcag gagctttttc ggaattttta gatgctatag agcatggtta
      301 tatattgcca ggaactacat tgttagttga aagtctggac agactttcaa gagaaaaagt
      361 cggtgaagcg attgaacgtc tgaaattgat tttgaatcac ggtattgatg ttataactct
      421 ttgcgacaat acagtctata atattgactc tttgaatgag ccatattcat taataaaagc
      481 catacttata gcacaaaggg caaatgaaga aagcgagata aagtcaagtc gggttaaatt
      541 atcatggaag aaaaaacggc aggatgcact ggaatcaggt acgattatga cggcgtcttg
      601 tccgagatgg ctctccttag atgacaaaag aacggctttt gttccagacc ccgacagggt
      661 gaaaactatt gagctaattt ttaaactcag gatggaaagg cgctcattga atgcaatagc
      721 caagtattta aatgatcatg ctgtaaagaa tttctcagga aaagaaagtg catgggggcc
      781 ttctgtaatt gaaaaattat tagcgaataa agctctgata ggtatatgcg taccttcata
      841 tcgtgcaaga gggaaaggga taagtgaaat cgctggctat tatcccagag tcatatcaga
      901 tgatttgttt tacgctgtac aggaaattcg gttggcacct tttggtatta gcaatagtag
      961 caagaatcct atgctaataa atctacttcg aacagttatg aagtgtgagg cttgtggtaa
     1021 taccatgatt gttcatgcgg tatctggaag tttgcatggc tattatgttt gtccgatgag
     1081 aagattacat cg
//
LOCUS       DABSYT020000348         1080 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.354, whole genome shotgun sequence.
ACCESSION   DABSYT020000348 DABSYT020000000
VERSION     DABSYT020000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.354"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..497
                     /locus_tag="ID725_RS26785"
                     /old_locus_tag="pgaptmp_005357"
     CDS             <1..497
                     /locus_tag="ID725_RS26785"
                     /old_locus_tag="pgaptmp_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853716.1"
                     /translation="EKQNLFLTVKNFKAEEKINQEKYIEKPANVAHEIASFFDIEQYL
                     LGGIDRNNNYINENFTYKFQDLANLSGTRDAQLERKVYFNEKMLNLNDNDEKVKIEND
                     IKKIDKKIEEIDNAIRDLSSETERFKIKYQQENTLNDVVQNIRTWLKENQNMVKAIKK
                     IDTE"
BASE COUNT          412 a          163 c          192 g          313 t
ORIGIN      
        1 tggaaaagca aaatcttttt ttaacggtga aaaactttaa ggcagaagaa aaaataaacc
       61 aagagaaata tatagaaaaa ccagcaaatg tagcccatga aattgcttca ttctttgaca
      121 ttgaacaata tttattgggg ggcattgata gaaataataa ttatattaat gaaaatttca
      181 cttataaatt tcaagattta gccaaccttt ctggcacaag agatgctcag ctagaaagaa
      241 aggtttattt taatgagaag atgcttaatt taaatgataa tgatgaaaaa gtcaaaatcg
      301 aaaatgatat aaagaaaata gataaaaaaa ttgaagaaat agataatgca atccgggatc
      361 tttcctctga aacagagagg ttcaagataa agtatcaaca agagaataca cttaatgatg
      421 tagttcaaaa tatcaggact tggctaaaag aaaatcaaaa tatggtgaaa gctattaaga
      481 aaattgatac tgaataacat ggcgaataaa taaacggatc aagtcgttcc tatgacaatg
      541 aggttatacg aaaagacgct tactcatcag aaatctggta tgctttaatt aaaccagtgt
      601 cgctgatgtg taagcgttga cgttgggaga cgattaaatg gttataccac tcctacgtct
      661 tttccatacg gcgtataaga gtgcaacatg ttgagttttc cgagtgaatg gtgcatttta
      721 ctaaccgagc catttgaatc aaccaacgca ataaaaaaga ctggaataat aagcctgtat
      781 ttattattta tcgcaatcgc actttacggt tttatccaac tgaaaagaga ctgcttccag
      841 cggttgtgga ttatccctgg gcgatagtca tagtctggtg tcaggattca caaatgacct
      901 gtttccttac atggatataa acacaacata gagtgcggta gttaatctga ataatctaat
      961 ttcggctatt cagcacaaaa caacattatt gattctttat aagagagaat ctctccatca
     1021 tttttatata aatcattatt aagaaaatcc agaagctctt gccttacact ataaatattg
//
LOCUS       DABSYT020000349         1055 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.355, whole genome shotgun sequence.
ACCESSION   DABSYT020000349 DABSYT020000000
VERSION     DABSYT020000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.355"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(246..524)
                     /locus_tag="ID725_RS26790"
                     /old_locus_tag="pgaptmp_005358"
     CDS             complement(246..524)
                     /locus_tag="ID725_RS26790"
                     /old_locus_tag="pgaptmp_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HGV7853717.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
     gene            952..>1055
                     /locus_tag="ID725_RS26795"
                     /old_locus_tag="pgaptmp_005359"
     CDS             952..>1055
                     /locus_tag="ID725_RS26795"
                     /old_locus_tag="pgaptmp_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853718.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGT"
BASE COUNT          302 a          200 c          213 g          340 t
ORIGIN      
        1 ttttcgtgtc tttaagtgat accagatggt attgcgctaa atgtaccaat gggtatacca
       61 agagcactaa aaacaaaggg gcaaccactt tgtaacccct tgattatttg cagaagcgca
      121 gaaaatcgaa ctctggaacc ctttcggggc atcctttttc aagaatgaag aaaaaaataa
      181 ctaaaaaatc aagttgttag gaagttattc atggctagtg taccaactct tttctgggct
      241 aatagctatg atttgtttgt attatttgtc tctgtctcta ttatttttga catccatgcg
      301 acttctgata ttgataacct cagttgtgga gttctgtctg cgcttccggg aggacgcata
      361 tcaaaactac aggatcttct gttaaacact gtaaagccat ctacattgca tgttgttgag
      421 tctaagatac aatttttagt agatgattta tctgctatag gcctagatat ttcaggttga
      481 tgccaagtgt gtcctgaaaa aatacttttt attgaaaatg gcatttttat cctcacatta
      541 tgtctttctc ttatagtgca atttaatgta tttagaaaca ttaaactatg aatctaaaaa
      601 ggctacgcag agtactgtta cactttaaca aatcatattt accactctgg aaatgggatg
      661 gctggttcat aaaataatga gtctttttca acggttgtta cattggtgta ctgatgatta
      721 tgccttgtcg ctaagagcta ccataaccgt acagcgtgat tcatccatgg cgaataaatc
      781 gactgataaa tccccctgag tttcagctct tgctgttgga ccttgcccgt ttcgctatgg
      841 aacgcgtacc agctagagaa tattattcgt tctcctccaa tgttacgcaa gttttgtaaa
      901 cggtaaccaa tcgtgattag tcctgcatgt aacggacatc aagctgtctg gttgattttt
      961 attgccagta gttcgagaga gcagacaact gcgtccgatg gaaaaatggg cacgacaggg
     1021 tattcaggca acctcgctga ggaggcttat ggtac
//
LOCUS       DABSYT020000350         1051 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.356, whole genome shotgun sequence.
ACCESSION   DABSYT020000350 DABSYT020000000
VERSION     DABSYT020000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.356"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
BASE COUNT          277 a          226 c          254 g          294 t
ORIGIN      
        1 acctattggc aagtgatttt tacccctgac ttaaaacaaa aaacagatta aatacatata
       61 gttaagttat gtttacatga tgcccggcag ggcatagaag gggcgaaaga aaataccagg
      121 gagcagcaga gccacatcgc cccgaagcgc ggcgcagccg ctgccgaacg taccagggcg
      181 cacaggccat cccttcgccc tgcaaagccc aacagaccag caggccatga ttatgctgat
      241 ttgcgccgca gaatgccgca gttagcgcga gcgtaagcga gtttacgccg tcctggtgcg
      301 tcgcagcagg gcggggcgct gtactgcgcg tgcgagcatc gcgagctacg ggtgcaatgc
      361 tactccgcta aaacgttctg aatcgcgttc tgagcaattt ttaatgcttc cctgtgggtt
      421 tggcttacca gggctaaaat aaagcgacag gacgtgtttt atggctttct ggggtggtta
      481 ggggaaggat cgttactgga tctgttaatt ttgtagttta tgctccgtca tgatgaatga
      541 tcatatctgg taactctttg gttggcgtat cctattgata tagaagtata tttcattgaa
      601 acatgagaag atcctcggtt agtgaaatga ggatcttgat taaaccccac caatataaaa
      661 tcaattaaat caatagatta actaattaca tcgatcacat taaacgttca ttttgatgaa
      721 cagagagctg ctgcaagcct tttgccccct tcattcactg aaagaattct gttttatttg
      781 tctaagtgct tctgagagtg ttttagcggt tattacggct tcgtttccga aagaacaata
      841 ccgaaattat cgacagtatg cgagagataa caccgctcgc cgcagcccta gcgaccgaac
      901 gttagtgagc gagtgggcga ggaagcggaa tttctgcgat gtaccattgg agcaagtacg
      961 ctttgtgctt tgaccgtacc tgatggtttt aatgtgttga gcagaattat tcacaattcg
     1021 ctctattatt tttctttatt tcttttttct a
//
LOCUS       DABSYT020000351         1042 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.357, whole genome shotgun sequence.
ACCESSION   DABSYT020000351 DABSYT020000000
VERSION     DABSYT020000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.357"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>1042
                     /gene="espN"
                     /locus_tag="ID725_RS26800"
                     /old_locus_tag="pgaptmp_005360"
     CDS             <1..>1042
                     /gene="espN"
                     /locus_tag="ID725_RS26800"
                     /old_locus_tag="pgaptmp_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HGV7853719.1"
                     /translation="IRQGIKHDDVNRIAENIIKKYGITEGMNPKPRNARISKELLLLA
                     VDRQIEWAKKHFITKDVLENVVSKCDLSSIFNVNKVLQNTILEFVHEINNISSARWMS
                     KSEKNNKQKEAIEKFKKEVSHMNGGQQFIWGFDKVIQEGLSGLIELSIDINDSTNHRD
                     KSSLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSSILHSAIVKESSNICDYIQGAV
                     RIGMDFVPGTRSDLHSRSLQIKYEALKHIEKNINDNIIYHPSNNANFYSVIESIDGND
                     FIYNEKQSKILEMKQDRGGNRYSAVDLNSSKYGYYEKVGGGFYRYIESFNPISSETPN
                     KIVYKGESVDLTK"
BASE COUNT          372 a          126 c          217 g          327 t
ORIGIN      
        1 atacgacagg gaattaaaca tgatgatgtt aatagaatag cggaaaatat aataaaaaag
       61 tatggtataa ctgagggtat gaatcctaaa cctaggaatg ccagaatatc taaagaattg
      121 cttttattag ctgttgatag acagattgag tgggcgaaaa aacattttat aacaaaagat
      181 gtattggaaa atgttgtgtc aaaatgtgat ttatcatcta tctttaatgt taataaagtg
      241 cttcagaata ctattcttga gtttgtccat gaaattaata atatatcatc tgctcgctgg
      301 atgtcaaaat cagaaaagaa taataaacaa aaagaggcaa tagaaaagtt caaaaaagaa
      361 gtatcccata tgaatggcgg gcagcagttt atttgggggt ttgataaggt tattcaagaa
      421 ggcttaagtg gattgattga gttaagtatc gatattaatg atagtacaaa tcatcgtgat
      481 aagtcttctc tttctcctga tgggagagct gtgttacatt ttttaggtac aatttggaat
      541 atggcgatgg gagctgtacc tggttataat gcattgtctg gtgtaagtag cattttacat
      601 agtgctatag taaaagaatc tagcaatatc tgtgattata ttcagggggc tgtacgtatt
      661 ggaatggact ttgttccagg cactcgctct gacttacata gccgttcgct gcagataaaa
      721 tatgaggctt tgaagcatat agaaaaaaac attaatgata atattattta tcatccgagt
      781 aataatgcta atttctattc tgtaattgag tcaattgatg gtaatgactt tatatataac
      841 gaaaaacaat ctaaaatatt agaaatgaaa caggatcgtg gggggaatag atatagtgca
      901 gtagatctta actcttctaa gtatgggtat tatgagaaag ttggcggtgg tttttataga
      961 tatatagaat cctttaaccc catatcttca gagacaccaa ataaaatagt ctacaagggg
     1021 gaatcagtag atttaactaa gg
//
LOCUS       DABSYT020000352         1033 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.358, whole genome shotgun sequence.
ACCESSION   DABSYT020000352 DABSYT020000000
VERSION     DABSYT020000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.358"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(219..386)
                     /locus_tag="ID725_RS26805"
                     /old_locus_tag="pgaptmp_005361"
     CDS             complement(219..386)
                     /locus_tag="ID725_RS26805"
                     /old_locus_tag="pgaptmp_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGV7853720.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(383..811)
                     /locus_tag="ID725_RS26810"
                     /old_locus_tag="pgaptmp_005362"
     CDS             complement(383..811)
                     /locus_tag="ID725_RS26810"
                     /old_locus_tag="pgaptmp_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HGV7853721.1"
                     /translation="MVFLVKKARRAATEIKKFEKRDLAQVVINAAYLVACADGECEAS
                     EKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTREA
                     EDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
BASE COUNT          261 a          309 c          233 g          230 t
ORIGIN      
        1 ccgcgccatg cgcagccgga aataaaggga taacgatgat agtttgagaa aaacagaaat
       61 aacacttttg tggcaaagca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      121 gaatccgcga ttatcccata taccttcttg ctgatcgccc ctccgcacag ggggattcac
      181 catgcagaag tgtttttaat aaacagcaaa caaaaaaatc aagcattatg caggctgttt
      241 ctttttatca ccggccacag caataccaca atgccgcaga ccagcacccc atccgccagc
      301 accgacatga ttctgctggt gaaatccacc atcaccacca gaaacagcag gagtgcagcc
      361 acagtcaggc gcagttttac cgtcacaggt gattctccag acgaagcccc agaacaccgg
      421 caatctcttc cagcaccttg cgctcttccg gctcaatttc gccgtctgcc tccgcaatgg
      481 ccaccgccac atccagcaca tcttccgctt cacgcgtatc gtgtttcaca tcctcgatct
      541 cacgtaacgc cgcacgacga ccagttttaa agttcgtatc cagctgaccg ataatggttg
      601 cgctaatcgc attaatttct gacgtaaacg cggacagcgc aggctgatta cgcagtacct
      661 gttcgatctt cgctttctcg gatgcctcac attcaccatc tgcacaggcc accaggtatg
      721 cggcgttaat caccacctgt gccagatcgc gtttttcaaa ctttttaatt tccgttgccg
      781 ctctgcgggc tttttttacc aaaaatacca aacatcgtga cgttcctttg ggtgggtgag
      841 ccaacgcccg ggagcgatct gcccacagag aaagtcacac tgaccactcc gtaagctccc
      901 ccccccccga aaggctctgt ggttggtatg tgccgggcgt ggcgcagata caaaaaaggc
      961 ccgcaaaagc gagccgggaa aataagtgtg gcgcgttgta ctggagtcga accagtgacc
     1021 gattgcttag aag
//
LOCUS       DABSYT020000353         1024 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.359, whole genome shotgun sequence.
ACCESSION   DABSYT020000353 DABSYT020000000
VERSION     DABSYT020000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.359"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..915
                     /locus_tag="ID725_RS26815"
                     /old_locus_tag="pgaptmp_005363"
     CDS             <1..915
                     /locus_tag="ID725_RS26815"
                     /old_locus_tag="pgaptmp_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGV7853722.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDIL
                     FTGGSGPDNKILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRP
                     SRTAEEEVQAQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWP
                     GKHADEIKNVKASLKNTLIDVKKKMHAN"
BASE COUNT          310 a          184 c          294 g          236 t
ORIGIN      
        1 aggctggagc gggaactgcg gcagggcagc gtgagtgaag aaagccagca gtggcttgcg
       61 cagtgcgggc tgacggcgga acagatggcc gcgcagctgg aggcggaata tatcccggag
      121 cggaaacttc atctttacca ctgcgaccac cggggactgc cgcaggcgct catcagcccg
      181 gaaggggaaa cggcgtggca gggggagtat gacgagtggg gaaacctgct gggcgaaacc
      241 agcgcgcagc acctgcaaca gccgtaccgt ctgccgggac agcagtatga tgaggagtcg
      301 gggctgtatt acaaccgtca ccggtactat gacccgctac aggggaggta tatcacccag
      361 gatccaatag atataaaagg aggatggaat ttatattctt atgcgcttaa tccggtaagt
      421 tggattgacc cattaggatt gacgcagtgc gattccgaag ggtgtaataa tgatatatta
      481 tttacaggag gaagtggtcc cgataataaa atacttaatg aattaggtcc gagagatggc
      541 attgacggtt tgggttcaca aaacatgaaa atgtatagtg ggctattagg gggcgatatt
      601 ttaaaaccgg ggattctggg cggtttgacg cttggtagtg tgacacaacg accatcgcga
      661 acggctgagg aggaggtgca agctcaaata gaatatttag catataagaa gcgttgtgag
      721 caaaaacctg atagtaattt aagtcgttgt gctgccgcga tctttcaaaa gagcagaaaa
      781 gaagattgtt taagaatgag acaagaatgg gatgataaat ggtggcctgg caaacatgcg
      841 gatgaaataa aaaatgtgaa agcatcgttg aaaaacacac ttattgatgt taaaaaaaag
      901 atgcatgcca actagtggca ttccgagata ggtatcctta aaatataatg atgttaatta
      961 tgcccattga ttggtgaaaa tgctataata tattttctta tctttaatat gctgccgatt
     1021 tatg
//
LOCUS       DABSYT020000354         1019 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.360, whole genome shotgun sequence.
ACCESSION   DABSYT020000354 DABSYT020000000
VERSION     DABSYT020000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.360"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..705)
                     /locus_tag="ID725_RS26820"
                     /old_locus_tag="pgaptmp_005364"
     CDS             complement(<1..705)
                     /locus_tag="ID725_RS26820"
                     /old_locus_tag="pgaptmp_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGV7853723.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTG"
     gene            complement(711..>1019)
                     /locus_tag="ID725_RS26825"
                     /old_locus_tag="pgaptmp_005365"
     CDS             complement(711..>1019)
                     /locus_tag="ID725_RS26825"
                     /old_locus_tag="pgaptmp_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGV7853724.1"
                     /translation="VISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCT
                     WTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ
                     "
BASE COUNT          224 a          325 c          269 g          201 t
ORIGIN      
        1 ccccgtaaag gcagatgcgc gccatgcccg gtgacgccag agggagtgtg tgcgtcgctg
       61 ccatttgtcg gtatacctct ctcgtttgct cagttgttca ggaatatggt gcagcagctc
      121 gccatcacca cagtaaatgg cggcatgatt cggcaccgat gaaccaaaac agcacagcag
      181 cacatcgccc ggttgtgctg atgacaacgg cacctgatac agccctgtgg cctccagatt
      241 atccagatag agattctgac cgtgacgcca ccagtcatcc tcgcgatgaa aatccggcat
      301 ctcaatcccc gccagatgat aagcatcccg gaacagcgtg taacagtccg tcaccccgtg
      361 ctcaaagcgc cgcccggtga gatgcggcac acagcggaac ttatgaatcg tcccccggca
      421 gaccagccac cacggcaaat cactctgcac ctgcagccgc cggtcggcct cactcagcca
      481 gggcagacca ccggggtggc tgtggaccag cgccacaatc tcaccctgca tttctgcctg
      541 cagccagtct tccggcgaca tacggaaata cgcctccggc tcaccggaga tattcacgca
      601 ggggaaatat ctttccccct ccggcgtgct taccacgaag ccgcacgact ccgctggcgc
      661 acatcgccgg gcgtgcgcca gaatcgctga ttctgtctgt gtcatgggat ttactgcgaa
      721 agtttgttaa tggaaaggaa gccgccaaag ttgccgacgt tattgcggaa cttacaaccg
      781 ctcaggcatt tgctgcattt atccttcgtg atatcggacg ttggctggtc atattcatcc
      841 gcgaccgccg gaccgtgata accgcactca tcgccgcgat aggtccaggt gcaggtgttg
      901 gccagcatga tacgtcccgg aaaaacagcg ccgtccgttt ccgtcggcgt ggacagtaca
      961 aaagaggcac tcaccgcgct cagttcgctg cactgctcaa tgcgccagcg gctgatcac
//
LOCUS       DABSYT020000355         1013 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.361, whole genome shotgun sequence.
ACCESSION   DABSYT020000355 DABSYT020000000
VERSION     DABSYT020000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.361"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..80)
                     /locus_tag="ID725_RS26830"
                     /old_locus_tag="pgaptmp_005366"
     CDS             complement(<1..80)
                     /locus_tag="ID725_RS26830"
                     /old_locus_tag="pgaptmp_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGV7853725.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEW"
     gene            complement(86..802)
                     /locus_tag="ID725_RS26835"
                     /old_locus_tag="pgaptmp_005367"
     CDS             complement(86..802)
                     /locus_tag="ID725_RS26835"
                     /old_locus_tag="pgaptmp_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGV7853726.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(861..>1013)
                     /locus_tag="ID725_RS26840"
                     /old_locus_tag="pgaptmp_005368"
     CDS             complement(861..>1013)
                     /locus_tag="ID725_RS26840"
                     /old_locus_tag="pgaptmp_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853727.1"
                     /translation="YAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENRCYRATL
                     EFQVTV"
BASE COUNT          226 a          297 c          243 g          247 t
ORIGIN      
        1 ctccattccg gcaccttcac cgttttatga cggaaccccg acagtctggc cagcgccagt
       61 gttttcagat cttttgccat aagccttatc cgcccgcacc attaaccgtt actgtacacg
      121 catcagaggt aatgctctgc ggctgttctg cagaatccgt tacctcgcag gtataagccc
      181 ccttatcacc tgactgcgta ttggctttac tgaaagtgtc agtagtctgt ccctctaccg
      241 gctgaccatc cttcttccag gcgtgtttat aaggcggcgt tcccccgttg acactgactg
      301 acattgtcag cagcgcaccg gtattcacgg taagtgtctt ctccagattt ttcacaaacg
      361 ccagcggtac cacataggac accggtttac ccttcaggcg aagtgaaaac gttgcagcca
      421 ccacgccgtt ggtaccggat gaccaggtgt gctgacgcac ttccgccagg aacttaaagc
      481 ccttaccgga cggaaactgc accttaaacg catacaccgt gtcattgtca taggcatcac
      541 gcagggcgtt ctgggcctga ttcagataaa aattacccga catggaaatc tcggacgacg
      601 cccccagacc gttgatgttc tcctgctctg tggagcagag cgtggtcaca tcaatatcct
      661 gtttctgacc ggcggtgaac tggacttcct tgatggtgca gtccaggcgc agatattccg
      721 ccttctccat ggtttcagca gtcgccgggg cagatgaaat catcacctgc gtcagctgtg
      781 aacgttcata caaagcagac attctgcctc ctgataataa aaaacccgca cgcggcgggt
      841 tatgggttct gttgaaaaaa ttacaccgtg acctgaaact ccagggttgc acggtaacag
      901 cggttttccg gaatatagtc ctgcatttca ctgacggatc ccggggccag cagcattatg
      961 gcttcacggg cgtcctgacg tatctgacgc gcctgcgtca cagtcccggc ata
//
LOCUS       DABSYT020000356         1001 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.362, whole genome shotgun sequence.
ACCESSION   DABSYT020000356 DABSYT020000000
VERSION     DABSYT020000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.362"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..506)
                     /locus_tag="ID725_RS26845"
                     /old_locus_tag="pgaptmp_005369"
     CDS             complement(<1..506)
                     /locus_tag="ID725_RS26845"
                     /old_locus_tag="pgaptmp_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HGV7853728.1"
                     /translation="MYLHIVPKLFHRMANKCSLKTISIPELDFIIDSESLSVGRPWPN
                     KCLWVGMRKGRKSVNGLVLQTDKKLRWFTTIYTWDIEDMGLIYHRVNTYIEDDDFDMV
                     SQEILLNGSFDKWSDRVHSAYENNPPARIQPKMESLLNKPGENSHDMWEEFEWGDFLL
                     SREESLLL"
     gene            complement(629..934)
                     /locus_tag="ID725_RS26850"
                     /old_locus_tag="pgaptmp_005370"
     CDS             complement(629..934)
                     /locus_tag="ID725_RS26850"
                     /old_locus_tag="pgaptmp_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HGV7853729.1"
                     /translation="MRKAKAIFTAIVFSLGAGSIPAYASDPCASVLCLYGKAVGQGGG
                     SECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVKG"
BASE COUNT          292 a          228 c          163 g          318 t
ORIGIN      
        1 tgaagtaaaa gactttcctc tctggataga aggaagtctc cccattcgaa ctcttcccac
       61 atatcatggc tgttttcacc aggtttattt aataaagatt ccattttggg ctgaattctg
      121 gctggaggat tattttcata agctgaatgc accctatcac tccacttatc aaaagaacca
      181 tttaagagaa tttcctgtga taccatatca aaatcatcat cttctatata ggtattgacc
      241 cgatgatata ttaatcccat atcttcaata tcccaggtgt atatagtagt aaaccatctt
      301 aattttttat ctgtttgcaa aacaaggcca ttaactgact ttcttccttt tcgcattcca
      361 acccataaac atttattagg ccacggtctg cctacagaca atgattcact atcaattata
      421 aaatcaagtt caggtatgga aatggtttta aggctacatt tattagccat gcgatgaaaa
      481 agtttaggaa caatatgcag atacatagtg gtttccttta tctcattttc aagtctggac
      541 ttattaccaa aaaatcacta acgcccttcg gttgttatgt gatacttgca gttccctgat
      601 acccgttggc gcgggtatca ggttttaatc agcccttaac gcgtccgaac ttactcataa
      661 tttttgagat agcagccgga tcagccgttg agcactgatt caaaaatgcc ttgcgggcat
      721 caaatgtttt actccagcga atgcttcctt ttttcttttt cagaatatta aaaaagtcct
      781 tttcagcact tctgcactca cttccacctc cttgcccgac agcttttcca tacaggcaaa
      841 gcaccgaagc gcaaggatca gaagcataag caggaatact ccccgccccc aatgaaaata
      901 cgatggcggt aaatatcgct ttagcttttc tcatatagtt caccagatat tattcagaaa
      961 catcctccag atcgtgtgtc tcaagatctg gtttagtcat a
//
LOCUS       DABSYT020000357         1000 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.363, whole genome shotgun sequence.
ACCESSION   DABSYT020000357 DABSYT020000000
VERSION     DABSYT020000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.363"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>1000
                     /locus_tag="ID725_RS26855"
                     /old_locus_tag="pgaptmp_005371"
     CDS             <1..>1000
                     /locus_tag="ID725_RS26855"
                     /old_locus_tag="pgaptmp_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HGV7853730.1"
                     /translation="KTSHEIKHIYSKSSKTREHYKALSEPEKKSFLKNRIDDYDKKYK
                     LNTKNSERTQGRSCGNKFSIESTSRFSRTKTRVGVPRLPQRGLVYGIHGRGKPPESVR
                     ILQDNEQRNLANRMENRSHSDSAMRRNADRRFSERGIKNIGISTPLHEALFQKLNNDA
                     ERNELTTMAEIRKKIDPERFLSATAREFNIVPGEHAISKAKDGSPRFAVGNRNLNASD
                     FLTKYINLSWSDAKNFLLKTYGEQLTEKTFEPVATTRKLSYEESRERYSSFKKTDISL
                     RSIIRAEKKNMYNELREMRQQIYSLPKENRDVAKGVLVYKKITTLERLDDMYAAGRS"
BASE COUNT          369 a          167 c          204 g          260 t
ORIGIN      
        1 aaaaccagtc acgaaataaa acatatttat tcaaaatcga gtaaaacgcg tgagcactat
       61 aaggcgctta gtgagccgga aaagaaaagt tttctaaaaa acaggattga tgactatgac
      121 aaaaaataca aacttaacac aaaaaattct gaaagaacgc aaggacggtc ttgtggtaac
      181 aagttcagta ttgaatcaac ttccagattc agtagaacta aaacaagagt cggcgtgcca
      241 cgtttgcccc aacgcggttt ggtttacgga atccacggga gaggaaaacc gcccgaatct
      301 gtccgtattt tgcaggataa tgaacagcgt aatctggcaa acagaatgga aaacagatcg
      361 cattctgatt cagcaatgcg acggaatgct gatagacgat tctcagaacg aggaataaaa
      421 aatatcggta tatcaacgcc attacatgaa gcattatttc agaagttaaa taatgatgca
      481 gagcgtaatg agttaaccac tatggcagaa atcagaaaga aaattgatcc tgaacgtttt
      541 ctttctgcga ctgcccgtga atttaatatt gtacctggag aacatgcaat cagtaaggca
      601 aaggatggtt ctccgcgttt tgcagttggc aacagaaatc ttaatgcgtc tgattttttg
      661 actaaatata ttaatttatc atggtctgat gctaaaaact tcttgcttaa aacatatggg
      721 gagcaattga ctgaaaaaac ctttgagcca gtagcaacaa caagaaaact aagttatgaa
      781 gaatccagag aaagatattc ttcctttaaa aaaacggaca tttctttaag aagtattatt
      841 cgtgcagaaa agaaaaatat gtataatgaa ttgcgcgaaa tgcggcaaca gatttattct
      901 ttacctaaag aaaatcgtga tgtagctaaa ggtgttttag tttataagaa aataaccact
      961 cttgaacgac tggatgatat gtatgctgcg ggacgtagtt
//
LOCUS       DABSYT020000358          963 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.364, whole genome shotgun sequence.
ACCESSION   DABSYT020000358 DABSYT020000000
VERSION     DABSYT020000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.364"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            85..699
                     /gene="evgA"
                     /locus_tag="ID725_RS26860"
                     /old_locus_tag="pgaptmp_005372"
     CDS             85..699
                     /gene="evgA"
                     /locus_tag="ID725_RS26860"
                     /old_locus_tag="pgaptmp_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGV7853731.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            704..>963
                     /locus_tag="ID725_RS26865"
                     /old_locus_tag="pgaptmp_005373"
     CDS             704..>963
                     /locus_tag="ID725_RS26865"
                     /old_locus_tag="pgaptmp_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HGV7853732.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADY"
BASE COUNT          300 a          184 c          203 g          276 t
ORIGIN      
        1 tttttattga gaaaatgaga tgatgcctta tgtctgtatt actacaggga gaagggagat
       61 gcttcattgc aaagggaata atctatgaac gcaataatta ttgatgacca tcctcttgct
      121 atcgcagcaa ttcgtaattt attgatcaaa aacgatattg aaatcttagc agagttgact
      181 gaaggcggaa gtgccgttca gcgggtggaa acacttaagc ctgatatcgt catcattgat
      241 gtcgatatcc ccggagttaa cggtatccag gtgttagaaa cgctgaggaa gcgccaatat
      301 agcggaatta ttattatcgt ctccgctaaa aatgaccatt tttacgggaa acattgtgct
      361 gatgctggcg ctaatggttt cgtgagtaaa aaagaaggca tgaacaatat cattgcggct
      421 attgaagctg caaaaaatgg ctactgctat ttccccttct ctctcaaccg gtttgttgga
      481 agtttaacgt ccgaccagca aaaactcgac tccttatcga aacaagaaat tagtgtcatg
      541 cggtatattc ttgatggcaa ggataataat gacattgctg aaaaaatgtt catcagcaac
      601 aaaactgtca gcacttataa aagtcgcctg atggaaaaat tagaatgtaa atcactgatg
      661 gatctttaca cattcgcaca acgtaacaaa atcggctaac cacatgaagt ttttacccta
      721 tatttttctt ctctgttgtg gtctttggtc gaccataagt ttcgcagacg aagattacat
      781 cgaatatcgt ggcatcagta gtaacaaccg tgtcacactt gatccactac gtctgagcaa
      841 caaggaatta cgttggttag cgagcaaaaa aaatcttgtg attgcagtac acaagtccca
      901 aacggctacg ttgttgcata ccgattcgca gcaacgggtt cgtggtatta atgctgatta
      961 ttt
//
LOCUS       DABSYT020000359          962 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.365, whole genome shotgun sequence.
ACCESSION   DABSYT020000359 DABSYT020000000
VERSION     DABSYT020000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.365"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..534
                     /gene="escU"
                     /locus_tag="ID725_RS26870"
                     /old_locus_tag="pgaptmp_005374"
     CDS             <1..534
                     /gene="escU"
                     /locus_tag="ID725_RS26870"
                     /old_locus_tag="pgaptmp_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HGV7853733.1"
                     /translation="GLNAYQALVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKK
                     MKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICL
                     YYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARTLYKNIHKGQYITED
                     FFEPVAQLIRIAIDLDY"
     gene            complement(531..>962)
                     /gene="etgA"
                     /locus_tag="ID725_RS26875"
                     /old_locus_tag="pgaptmp_005375"
     CDS             complement(531..>962)
                     /gene="etgA"
                     /locus_tag="ID725_RS26875"
                     /old_locus_tag="pgaptmp_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HGV7853734.1"
                     /translation="LISNYCYASDCFEITGKAYNIDPLILKAIAWNESKCKSGIKSKT
                     NKNGTYDIGIMQINSSHLDLLSKFNISEDDLLNDACINISVAGYILASNIKSRGNTWD
                     AVGAYNAGYFNTPNAVELRRQYAMKIYKTYNKLKNNEQIID"
BASE COUNT          299 a          160 c          164 g          339 t
ORIGIN      
        1 ggactgaacg catatcaagc tcttgtcgtt gttgcctttt ttgtttttct tttatggaaa
       61 ggcgtgctat tcggatatct actcttttca gtatttgatt tctggttcca gaagcatgag
      121 ggactgaaga aaatgaaaat gagtaaagat gaggtgaaac gagaagccaa ggatactgat
      181 ggtaaccctg aaattaaagg ggagcgccgt cgccttcatt ccgagataca aagtggaagt
      241 ttggctaata acatcaaaaa atcaaccgtt attgttaaaa acccgactca cattgcgatt
      301 tgcctatact ataaacttgg ggagactcca ttacctttag ttattgaaac aggaaaagat
      361 gccaaagctc tacagatcat taaactggct gaactctatg atattccagt gattgaagat
      421 attcctttag caagaactct ctataagaat atacataaag gacaatatat aacagaagac
      481 ttttttgaac ctgtggcaca attgattcgt attgcgatag accttgatta ttaatcgata
      541 atttgctcat tattctttaa tttattatat gttttatata ttttcattgc gtattgcctt
      601 ctgagttcta ctgcatttgg tgtattaaag taacctgcat tatacgcacc aactgcatcc
      661 catgtattcc ctctggattt tatgttagag gctaagatat aaccagcaac agatatgttt
      721 atacaggcat cattcaataa atcatcttca gatatattaa atttgcttaa taaatctaaa
      781 tgagatgaat taatttgcat tatacctata tcatacgttc cgtttttgtt tgttttgctt
      841 tttattccgc ttttgcattt tgattcattc catgcaattg cttttaatat taatggatct
      901 atgttatatg cttttcctgt aatttcaaaa caatcggaag cataacagta atttgaaatg
      961 ag
//
LOCUS       DABSYT020000360          960 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.366, whole genome shotgun sequence.
ACCESSION   DABSYT020000360 DABSYT020000000
VERSION     DABSYT020000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.366"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            89..>960
                     /locus_tag="ID725_RS26880"
                     /old_locus_tag="pgaptmp_005376"
     CDS             89..>960
                     /locus_tag="ID725_RS26880"
                     /old_locus_tag="pgaptmp_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853735.1"
                     /translation="MTIAKDSSTDEIIHGNDLRGMDDFYIKTTSFECPYEPCKIKATP
                     CSFTMKHVNQSYFRYGDKHKDGCGIHDPRYKNNHTSNDERKHNSPPAPVISLLKIDVK
                     PRGGVKNARNIKNENHKDEKKGNEHPVSSSSIKPVVDYYINNSNHNEQLSIPPYGTRS
                     YKDTFQLIFYKNNIRYYKPAIYYGVAQSNIRLHEDSDKHCITFLARDKKTQKPFTLEI
                     DVSDWNKSQKDVFWKEYEKQRKEADRYYKGLKDKRNAKKYLTVFFFGMPDENDKFLFK
                     TNHFKLVYVAFLGK"
BASE COUNT          358 a          139 c          177 g          286 t
ORIGIN      
        1 gtaagttgga gtacaatttt ttgattggat cgtcaataag aaaaagctag aatccttaga
       61 aggacataga ttttcatagg gttaagatat gacgattgcg aaggatagtt cgactgatga
      121 aatcattcat ggaaatgatc tccgtggaat ggatgatttt tacattaaga caacgagttt
      181 tgaatgccca tacgagcctt gtaaaattaa agccacacct tgtagtttta ctatgaaaca
      241 tgttaatcaa tcatatttca gatatggcga taaacataag gatggatgtg gtattcatga
      301 tccaagatat aaaaacaatc atacttcaaa tgacgaaaga aaacataatt cgccacctgc
      361 tccggttata tcacttttaa aaattgatgt gaaaccaaga ggtggtgtta aaaatgccag
      421 aaatattaaa aatgaaaatc acaaggatga aaaaaaaggt aatgaacatc ctgtatcttc
      481 atcaagtatt aagccagtcg ttgactatta cataaataat agcaatcata atgaacagct
      541 atcaatccct ccatatggca cgcgatctta taaagataca tttcaactta ttttttacaa
      601 aaacaacata cgttattaca aacctgctat ttattatggt gtagcccagt ctaatattcg
      661 tctgcatgaa gattcagata aacattgtat tactttcctc gcaagggata agaaaaccca
      721 aaaaccattt acgcttgaaa ttgatgtttc tgactggaat aaaagtcaga aggatgtgtt
      781 ttggaaggaa tatgaaaagc aaaggaagga agctgatagg tattataagg ggcttaaaga
      841 taaaaggaat gctaaaaaat acttgacggt tttctttttt gggatgccgg atgaaaatga
      901 taagtttcta tttaagacaa accactttaa attagtatat gtggctttct tgggtaaatt
//
LOCUS       DABSYT020000361          960 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.367, whole genome shotgun sequence.
ACCESSION   DABSYT020000361 DABSYT020000000
VERSION     DABSYT020000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.367"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            117..641
                     /gene="aroL"
                     /locus_tag="ID725_RS26885"
                     /old_locus_tag="pgaptmp_005377"
     CDS             117..641
                     /gene="aroL"
                     /locus_tag="ID725_RS26885"
                     /old_locus_tag="pgaptmp_005377"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGV7853736.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            691..882
                     /gene="yaiA"
                     /locus_tag="ID725_RS26890"
                     /old_locus_tag="pgaptmp_005378"
     CDS             691..882
                     /gene="yaiA"
                     /locus_tag="ID725_RS26890"
                     /old_locus_tag="pgaptmp_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGV7853737.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
BASE COUNT          263 a          228 c          243 g          226 t
ORIGIN      
        1 ttcactttaa gtggaatttt ttctttacaa tcgaaattgt actagtttga tggtatgatc
       61 gctattctca tgacaccggc tttcgccgca ttgcgaccta ttggggaaaa cccacgatga
      121 cacaacctct ttttctgatc gggcctcggg gctgtggtaa aacaacggtc ggaatggccc
      181 ttgccgattc gcttaaccgt cggtttgtcg ataccgatca gtggttgcaa tcacagctca
      241 atatgacggt cgcggagatc gtcgaaaggg aagagtgggc gggatttcgc gccagagaaa
      301 cggcggcgct ggaagcggta actgcgccat ccaccgttat cgctacaggc ggcggcatta
      361 ttctgacgga atttaatcgt cacttcatgc aaaataacgg gatcgtggtt tatttgtgtg
      421 cgccagtatc agtcctggtt aaccgactgc aagctgcacc ggaagaagat ttacggccaa
      481 ccttaacggg aaaaccgctg agcgaagaag ttcaggaagt gctggaagaa cgcgatgcgc
      541 tatatcgcga agttgcgcat attatcatcg acgcaacaaa cgaacccagc caggtgattt
      601 ctgaaattcg cagcgccctg gcgcagacga tcaattgttg attttcgagc gcctatactt
      661 aacgttcatc ccgtgaaata aggaagaacg atgccaacga aaccgcctta tcctcgtgaa
      721 gcatatatag tgacaattga aaaaggaaag ccaggacaga cggtaacctg gtaccaactc
      781 agagccgatc atcctaaacc agactcgttg atcagtgaac atccgaccgc tcaggaagcg
      841 atggatgcga aaaaacgcta tgaggaccct gacaaagagt gaccgcatca gactgctcgg
      901 aagggattct gagtgccact acaagggatc tgcgtcacat ttttcataat tcatgttttt
//
LOCUS       DABSYT020000362          957 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.368, whole genome shotgun sequence.
ACCESSION   DABSYT020000362 DABSYT020000000
VERSION     DABSYT020000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.368"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(23..313)
                     /locus_tag="ID725_RS26895"
                     /old_locus_tag="pgaptmp_005379"
     CDS             complement(23..313)
                     /locus_tag="ID725_RS26895"
                     /old_locus_tag="pgaptmp_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGV7853738.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(310..>957)
                     /locus_tag="ID725_RS26900"
                     /old_locus_tag="pgaptmp_005380"
     CDS             complement(310..>957)
                     /locus_tag="ID725_RS26900"
                     /old_locus_tag="pgaptmp_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGV7853739.1"
                     /translation="IANNMPEQYDEKPQVQQVAQIINGVFSQLLATFPASLANRDQNE
                     LNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPSPGQFVAWCREEASVIAGL
                     PNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNLYQNMRANALTDAELRRKA
                     ADELTCMTARINRGETIPEPVKQLPVMGGRPLNRVQALAKIAEIKAKFGLKGASV"
BASE COUNT          190 a          278 c          228 g          261 t
ORIGIN      
        1 attagctcag tgatgtagat ggtcatcttt taactccata taccgccaat acccgtttca
       61 tcgcggcact ctggcgacac tccttaaaaa tcaggttcgt gctcaccttt ccttcccgtt
      121 cttccctggt agcaaaccgg taatacaccg ttcgccagac cttaccttcg ataaccagaa
      181 gacctgcccg tgccatttta gctgcggcct gatttatgct ggttactgtt gcgcctgtta
      241 gcgcggcaac gtccggcgca cagaagctat tatgcgtccc caggtaatga ataattgcct
      301 ctttgcccgt catacacttg ctcctttcag tccgaactta gctttaattt ctgcgatctt
      361 cgccagcgcc tgaacacgat ttagaggtct gccgcccatg acaggaagtt gttttactgg
      421 ttcaggtatc gtctcaccac ggttaattcg cgctgtcata caggtcagtt catcggcagc
      481 cttgcgtcgt aattccgcgt cagtcagcgc attggcccgc atgttctggt acaggttggt
      541 aaccagccag tagtgcgcgt tcgatttcca cggataagac tccgcatccg gatacaggcc
      601 tcgcttccgg caatactcgt aaaccatatc aaccagctcg ctgacgtttg gcagtccggc
      661 gataacggat gcttcttccc ggcaccatgc aacaaactgc ccgggtgatg gcagaaatgg
      721 tcgattctgc cgacgggcta cgcgcattcc tgcgttaacc tgttccattg tggtgatccc
      781 gttttcccgg aaagccagaa cccactggcg gcggatttca ttcagttcgt tctggtcccg
      841 gttagccaga ctcgccggga aagttgccag taactggctg aacacaccgt tgatgatctg
      901 cgctacctgt tgtacctgcg gcttttcgtc gtactgttcc ggcatgttgt tggcgat
//
LOCUS       DABSYT020000363          945 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.369, whole genome shotgun sequence.
ACCESSION   DABSYT020000363 DABSYT020000000
VERSION     DABSYT020000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.369"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..264
                     /locus_tag="ID725_RS26905"
                     /old_locus_tag="pgaptmp_005381"
     CDS             <1..264
                     /locus_tag="ID725_RS26905"
                     /old_locus_tag="pgaptmp_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853740.1"
                     /translation="ILKKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENGII
                     PEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            378..>945
                     /locus_tag="ID725_RS26910"
                     /old_locus_tag="pgaptmp_005382"
     CDS             378..>945
                     /locus_tag="ID725_RS26910"
                     /old_locus_tag="pgaptmp_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGV7853741.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEGLPHP
                     LTREPITASIIVKHEECIYDDTRGNFIIK"
BASE COUNT          256 a          171 c          254 g          264 t
ORIGIN      
        1 atactaaaaa aacttatgca gcgtctgtgt ggttgcggaa agcatgatga ccgtgaacac
       61 ggggagttac ttacagcaca gctgcgactg gggccggcag acatcctgga gtcagatgag
      121 aatggtatta tcccggagca ggacagggta atcacgcagg tggtgatact ggatgcggat
      181 aaaaagcaga tacagtgtgt ggtaagaccg ctgcaaatcc tgcgtgctga cgggacgtgg
      241 gaaaatattg gcgggatgaa atagccgaca gcttcacaaa aaccggagtc cggctccggt
      301 tttttgttgt cacgtatggg ggatgtttgt tacagctatt taagtctgga gttcaaatta
      361 aaatagggag ctttgttatg ccattaacct cagatattag atcacattca tttaatcttg
      421 gggtggaggt tgttcgtgcc cgaattgtag ccaatgggcg cggagatatt acagtcggtg
      481 gtgaaactgt cagtattgtg tatgattcta ctaatgggcg cttttcatcc agtggcggta
      541 atggcggatt gctttctgag ttattgcttt tgggatttaa tagtggtcct cgagcccttg
      601 gtgagagaat gctaagtatg ctttcggact caggtgaagc acaatcgcaa gagagtattc
      661 agaacaaaat atctcaatgt aagttttctg tttgtccaga gagacttcag tgcccgcttg
      721 aggctattca gtgtccaatt acactggagc agcctgaaaa aggtattttt gtgaagaatt
      781 cagatggttc agatgtatgt actttatttg atgccgctgc attttctcgt ttggttggtg
      841 aaggcttacc ccacccactg acccgggaac caataacggc atcaataatt gtaaaacatg
      901 aagaatgcat ttatgacgat accagaggaa acttcattat aaagg
//
LOCUS       DABSYT020000364          935 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.370, whole genome shotgun sequence.
ACCESSION   DABSYT020000364 DABSYT020000000
VERSION     DABSYT020000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.370"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>935
                     /locus_tag="ID725_RS26915"
                     /old_locus_tag="pgaptmp_005383"
     CDS             <1..>935
                     /locus_tag="ID725_RS26915"
                     /old_locus_tag="pgaptmp_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGV7853742.1"
                     /translation="RSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQL
                     NPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAG
                     RLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRR
                     AYDEPGRLVSETSRCGDVIRYAYDNPHSELPATTTDATGSTRQMTWSRYGQLLAFTDC
                     SGYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGHETRYEYNAAGDLT
                     AVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT          212 a          259 c          328 g          136 t
ORIGIN      
        1 cgcagcaata cgcaggtgcg cgctttcacg tatgacgcgc agcatccggg ccggatggtg
       61 gcgcaccgtt acgcgggaag gccggagatg cgctaccgct acgacgatac cgggcgggtg
      121 gtggagcagc tgaacccggc aggcctgagt taccgctacc agtatgagca ggaccgcatc
      181 accgtcacgg acagcctgaa ccggcgtgag gtgctgcata cagaaggcgg ggccgggctg
      241 aagcgggtgg tgaaaaaaga actggcggac ggcagcgtca cgcacagcgg gtatgacgcg
      301 gcaggaaggc tcacggcgca gacggacgcg gcgggacggc ggacagagta cggtctgaat
      361 gtggtgtccg gcgatatcac ggacatcacc acaccggacg ggcgggagac gaaattttac
      421 tataacgacg ggaaccagct gacggcggtg gtgtccccgg acgggctgga gagccgccgg
      481 gcatatgatg aaccgggcag gctggtatcg gagacatcgc gctgtgggga cgtcatccgg
      541 tatgcttatg ataatccgca cagtgaatta ccggccacga caacagatgc gacgggcagc
      601 acccggcaga tgacctggag ccgctacggg cagttgctgg cgttcaccga ctgctcgggc
      661 taccagaccc gttatgaata cgaccgcttt ggtcagatga cggcggtcca ccgtgaggaa
      721 ggtatcagcc gttaccgccg ctatgacaac cgtggccggt taacctcggt gaaagacgca
      781 cagggccatg aaacgcggta tgagtacaac gccgcaggcg acctgactgc cgttatcact
      841 ccggacggca accggagcga gacacagtac gatgcgtggg gaaaagcggt cagcaccacg
      901 cagggcgggc tgacgcgcag tatggagtat gacct
//
LOCUS       DABSYT020000365          923 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.371, whole genome shotgun sequence.
ACCESSION   DABSYT020000365 DABSYT020000000
VERSION     DABSYT020000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.371"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            85..885
                     /locus_tag="ID725_RS26920"
                     /old_locus_tag="pgaptmp_005384"
     CDS             85..885
                     /locus_tag="ID725_RS26920"
                     /old_locus_tag="pgaptmp_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853743.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          292 a          177 c          209 g          245 t
ORIGIN      
        1 attatttaag tggggtatat aattagagtc cgttatcaat gctaaatatt ctaatcatta
       61 tgacaggcga gggagtgtcc aattatgaat tcaaaaaagc tttgttgcat atgtgtgtta
      121 ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg atgaaaagaa gaaaaaagct
      181 caagtcacac aaagtaatat taataaaaac actccccagc aactgacaga caaagattta
      241 ttcggtaatg aaaccactct ggccgtatcc gaagaggata ttcaagcagc gcttgatgga
      301 gatgagttcc gcgttcccct taattctcct gtaatacttg ttcaatccgg caaccgcgca
      361 ccggaaacca ttatgcagga ggagatgcga aaatattata ctgtttccac attctctggt
      421 atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca aagataaaaa tgagaacgag
      481 gatgttgccc gtgctgagaa tatgaactgg atgcaggcac tgcgttttgt ggctgcaaaa
      541 ggacatcaga aagcgattat tgtttaccag gatatgttgc agacaggaaa atatgactct
      601 gcgctgaaat caacagtatg gtccgactat aaaaatgaaa aactcactga cgctatctcc
      661 ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa caggtgagtg ggctacctgg
      721 tcgccggtga attatgaatc gagagtaatt tttccgccgg ctggaataac aaaaacgagc
      781 aataaagaac tatcaaatga ccatgttacg gaagcgcaac tgtttaattt gaaacaaaaa
      841 acatatgtat cgatggtgaa agacttggtg agtcgattcc agtaatcgtt attgttatgc
      901 tgccgcctgt agtttagggt ggc
//
LOCUS       DABSYT020000366          922 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.372, whole genome shotgun sequence.
ACCESSION   DABSYT020000366 DABSYT020000000
VERSION     DABSYT020000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.372"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            2..532
                     /locus_tag="ID725_RS26925"
                     /old_locus_tag="pgaptmp_005385"
     CDS             2..532
                     /locus_tag="ID725_RS26925"
                     /old_locus_tag="pgaptmp_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGV7853744.1"
                     /translation="MLAGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPAGFVAVFNS
                     NEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPDGECQKWNGTSWAKDA
                     EAEKLFRVREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRVLLNR
                     VDTTVAADVEWPVAPQ"
     gene            complement(576..>922)
                     /locus_tag="ID725_RS26930"
                     /old_locus_tag="pgaptmp_005386"
     CDS             complement(576..>922)
                     /locus_tag="ID725_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853745.1"
                     /translation="KIINKFGCEIGLGANIGKGLTIPHHAGIVVHFAVDAGENLVLRQ
                     NTTIGQIDGDMPSSRVKIGSNVDIGANCCIIGLSRKIGDNVKIGAMSFINKDIPSNCT
                     YITKKSGVVLYK"
BASE COUNT          267 a          196 c          201 g          258 t
ORIGIN      
        1 tctgctggcc ggaactaatg aatttattgg tgaaggtgac gcatacattc cgcctcatac
       61 aggtctgcca gcaaacagta ccgatattgc accgccagat attccggctg gcttcgtggc
      121 tgttttcaac agtaatgagg catcgtggca tctcgttgaa gatcatcggg gtaaaacggt
      181 ttatgacgtg gcttccggcg acgcgttatt tatttctgaa ctcggtccgt taccggaaaa
      241 tgttacctgg ttgtcgccgg atggagagtg tcagaagtgg aacggcacat cctgggcgaa
      301 agatgcagaa gcagaaaaac tgtttcgggt acgggaggca gaagaaacaa aaaacagcct
      361 gatgcaggta gccagtgagc atattgcgcc acttcaggat gccgtagatt tggatattgc
      421 gacggaggaa gaggcatcgt tactggctgc atggaagaca tatcgggtat tgttgaatcg
      481 tgttgataca acagtagcag cggatgttga gtggccagtc gccccacaat aaaaagaaaa
      541 agccatcgac agaaatatcg atggctttat gtactctatt tatacaatac aacaccactc
      601 tttttagtta tatatgtgca gtttgatggt atatctttat ttataaaaga cattgcacct
      661 atttttacat tatccccaat tttacgtgat aatccaatga tgcaacaatt agctccgata
      721 tcaacgttac taccaatttt tactcttgaa ctaggcatat caccatctat ctgtccaatg
      781 gtagtattct gtcgtaatac cagattttca cccgcatcaa cagcaaaatg aacaacaatt
      841 ccagcatgat ggggaattgt caaccctttt ccaatatttg cgcccaatcc tatttcacaa
      901 ccaaacttgt taattatttt ac
//
LOCUS       DABSYT020000367          915 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.373, whole genome shotgun sequence.
ACCESSION   DABSYT020000367 DABSYT020000000
VERSION     DABSYT020000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.373"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..642
                     /locus_tag="ID725_RS26935"
                     /old_locus_tag="pgaptmp_005387"
     CDS             <1..642
                     /locus_tag="ID725_RS26935"
                     /old_locus_tag="pgaptmp_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853746.1"
                     /translation="SAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPK
                     GDKGERGDTGPAGATGERGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTG
                     ATGPAGPQGPKGETGAAGPVGATGPQGAKGDPGETQIRFRLGPMRIIETNSYGWFPGT
                     DGALITGLTFLDPKDATQVQGMFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            644..913
                     /locus_tag="ID725_RS26940"
                     /old_locus_tag="pgaptmp_005388"
     CDS             644..913
                     /locus_tag="ID725_RS26940"
                     /old_locus_tag="pgaptmp_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGV7853747.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGRSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          247 a          198 c          330 g          140 t
ORIGIN      
        1 tcagcagaaa gcgcacagtc agcggaacaa agcagaatag cggcggaaga cgccgtaaac
       61 agaattccca ccgtggtggg gcctcccgga ccaaaggggg aaccgggtcc cgcgggtcct
      121 caggggccga agggagataa aggagagcgt ggagacaccg gtccggcagg ggcaaccggt
      181 gaacggggac cgggaggaga tacaggtccg gcaggtccgc aggggccgaa aggcgacagg
      241 ggagagcggg gagagaccgg tctgacagga agtacaggtc cacaggggcc aaagggagat
      301 accggggcaa caggtccggc aggaccgcag ggaccgaaag gggaaacagg tgcggctggc
      361 ccggtggggg ctaccggacc tcagggggcg aagggcgacc cgggggagac acaaatacgg
      421 ttccgtctgg ggccgatgag aattattgag acaaacagct atggctggtt cccgggtaca
      481 gatggtgcgc tcatcaccgg actgaccttt cttgacccca aagatgccac acaggttcag
      541 gggatgtttc agcatttgca ggtcagattt ggtgacgggc catggcagga tgttaaggga
      601 ctggatgaag tgggcagtga tacaggcaga actggagaat gacatgaata ttttgagaaa
      661 gcttatgcag agtctgtgcg gttgcggaaa gcatgatgac tgtgaaaacg gacggtcact
      721 tacagcacaa ttgcgactgg gaccggcaga cattctggag tcagatgaga atggcattat
      781 tccggagcag gacagggtaa tcacacaggt ggtgatactg gatgcagata aaaagcagat
      841 acagtgtgtg gtaagaccgc tgcaaatcct gcgtgctgac gggacgtggg aaaatattgg
      901 cgggatgaag taacc
//
LOCUS       DABSYT020000368          905 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.374, whole genome shotgun sequence.
ACCESSION   DABSYT020000368 DABSYT020000000
VERSION     DABSYT020000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.374"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..228
                     /locus_tag="ID725_RS26945"
                     /old_locus_tag="pgaptmp_005389"
     CDS             <1..228
                     /locus_tag="ID725_RS26945"
                     /old_locus_tag="pgaptmp_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853748.1"
                     /translation="NMKKSTFPVIVSTTGHAFSVARVTLCTICLKHEKTGKDYVVIFT
                     DSNNIRDYKTGVVPCFGELYQEDVDLIVGKS"
     gene            279..545
                     /locus_tag="ID725_RS26950"
                     /old_locus_tag="pgaptmp_005390"
     CDS             279..545
                     /locus_tag="ID725_RS26950"
                     /old_locus_tag="pgaptmp_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853749.1"
                     /translation="MKIRKGDRQYYLNKEGNMFHLVKRVKTFSKSATLGKTKATVITV
                     ADLIFHEDAFDTIDFTRGGLRENDKEVVSMMIQEMNNEREKNVN"
     gene            535..777
                     /locus_tag="ID725_RS26955"
                     /old_locus_tag="pgaptmp_005391"
     CDS             535..777
                     /locus_tag="ID725_RS26955"
                     /old_locus_tag="pgaptmp_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGV7853750.1"
                     /translation="MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGT
                     ASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE"
BASE COUNT          291 a          179 c          209 g          226 t
ORIGIN      
        1 aatatgaaaa aatcaacttt tcctgttatc gtttctacga ctggtcatgc gttttctgtt
       61 gcgcgtgtga cgctttgcac tatctgcctg aaacatgaaa aaacaggcaa ggattatgtt
      121 gttattttca cggatagcaa taatatccgc gattataaaa cgggagtagt cccgtgtttt
      181 ggagagcttt accaggaaga tgtcgatttg attgttggaa aaagttaaag taaaggggct
      241 ataagcccct ttactgttaa aataggaacc atattaatat gaagatacga aaaggtgaca
      301 ggcaatatta tttgaataaa gaaggtaata tgtttcatct tgtgaaacgg gtgaagacct
      361 tttctaaatc tgcgacttta gggaaaacaa aagcgacagt gataactgtt gccgatctaa
      421 tttttcatga agatgcattc gacactatcg actttaccag aggcgggtta agagagaacg
      481 acaaagaagt tgtttctatg atgattcagg aaatgaacaa tgaacgagaa aaaaatgtta
      541 actgaaaata caccagaaaa cattaaacgt ctgcggcaaa aaattggcct gacgcaaaga
      601 gaatgtgctg aaatattcag catgaacccg cgaacctggc gtcgcaagga agaacctgcc
      661 ggaactgcaa gcggtacagc acttacacca gtagagttta aatatcttca gcttttagct
      721 ggggaacatc ctgaatacgt cctgcgcaaa agggataaac ctaaatgtag tgaatgagtt
      781 gttgcgcccg tagctcgcga tgctcacacg cgcagtacag cgccccgccc tgctgcgacg
      841 caccaggacg gcgtaaactc gcttacgctc gcgctaactg cggcattctg cggcgcaaat
      901 cagca
//
LOCUS       DABSYT020000369          903 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.375, whole genome shotgun sequence.
ACCESSION   DABSYT020000369 DABSYT020000000
VERSION     DABSYT020000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.375"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..843
                     /locus_tag="ID725_RS26960"
                     /old_locus_tag="pgaptmp_005392"
     CDS             <1..843
                     /locus_tag="ID725_RS26960"
                     /old_locus_tag="pgaptmp_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGV7853751.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
BASE COUNT          210 a          223 c          261 g          209 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatatgca gggaaatacc
      481 cctgtgacgc ctctggtcga tgccggatgc aatatggtga ttgtgacgca tctgagtgac
      541 ggttctttat gggatcgcca ggcttttccg gacaccacaa tccttgagat ccgtccccgg
      601 aaaaggctga aatatgcagg tgatggtggc aacagcggcg gtctgctcag ttttacatcg
      661 gcacataccg acgcctggcg tcagcagggc tatgaagaca cgatgctggc gatggagcat
      721 atccggaaac cgctggcagc acgtcaggca ctgacccggt cagaggcggt attgcagaaa
      781 agcctggata taacggaaga ggcagatttg gcactgagaa acgcgatggc ccggattaaa
      841 taaagacgcc ccaaagaaac gccaccgcct gtggtggtgt ttttcctgtc tggtgttatt
      901 gaa
//
LOCUS       DABSYT020000370          903 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.376, whole genome shotgun sequence.
ACCESSION   DABSYT020000370 DABSYT020000000
VERSION     DABSYT020000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.376"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(181..804)
                     /locus_tag="ID725_RS26965"
                     /old_locus_tag="pgaptmp_005393"
     CDS             complement(181..804)
                     /locus_tag="ID725_RS26965"
                     /old_locus_tag="pgaptmp_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGV7853752.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(801..>903)
                     /locus_tag="ID725_RS26970"
                     /old_locus_tag="pgaptmp_005394"
     CDS             complement(801..>903)
                     /locus_tag="ID725_RS26970"
                     /old_locus_tag="pgaptmp_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853753.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
BASE COUNT          212 a          232 c          204 g          255 t
ORIGIN      
        1 gcagtcagca tgtttactac acaaatacaa ttcagagcat aaaaactact cggcggcagg
       61 ttattgagac tcatcaatga catgtaaaaa acgcccatta ttggtgtcaa gtttccccaa
      121 agttattcaa aaagtcaata ttatgccgtt aatatgttgc catccgtggc aatcatggcg
      181 ctaacgtgtg atcgcattca aaatgttgtc tgcgattgac tcttccttgt ggcattgcac
      241 aaccagagcg tcatacagcg gcttaacagt gcgtgaccag gtgggttggg taaggtttgg
      301 gattagcatc gtcacagcgc gatatgcggc gcttgctggc attcttgaat aaccgacgcc
      361 tttacatctt ccgcactctt tctcagcaac tatcccccac tgctctgttt tggctatatc
      421 aaccgcacgg cctgtaccgt ggcaatctct gcatcttgcg cccggcgtag cggcactacg
      481 gcaataatcc gcataagcga atgttgcgag cacttgcagt acctttgcct tagtatttcc
      541 ttcaagcttt gccacgccac ggtatttccc cgataccttg tgtgcaaatt gcatcagata
      601 gttgatagcc ttttgtttgt cgttctggct gagttcgtgc ttaccacaga atacagccat
      661 tccgaatccg gcttgtgatt gcgccatccc catagcagcc atcacatcag taccggaaag
      721 agagtcagaa gccgtggccc gtggtgagtc gctcatcatc gggctttttg gcgaatgaaa
      781 tttagctacg ctttcgagtc tcatgcgcct tctccctgta cctgaatcaa tgtgaggttt
      841 ccgcagaaca ctgcgccagt atcgatatac atctggttgg caaatttgag tggtttcact
      901 gct
//
LOCUS       DABSYT020000371          900 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.377, whole genome shotgun sequence.
ACCESSION   DABSYT020000371 DABSYT020000000
VERSION     DABSYT020000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.377"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            37..669
                     /locus_tag="ID725_RS26975"
                     /old_locus_tag="pgaptmp_005395"
     CDS             37..669
                     /locus_tag="ID725_RS26975"
                     /old_locus_tag="pgaptmp_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGV7853754.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGAELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
     gene            complement(688..>900)
                     /locus_tag="ID725_RS26980"
                     /old_locus_tag="pgaptmp_005396"
     CDS             complement(688..>900)
                     /locus_tag="ID725_RS26980"
                     /old_locus_tag="pgaptmp_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGV7853755.1"
                     /translation="LLDNPCLNIKIGTEILYKHFSRCGMTWQCLGTYNAGFAMDNQKK
                     RLQYAKKIYIVYTRLNELDNRKALAK"
BASE COUNT          300 a          145 c          182 g          273 t
ORIGIN      
        1 tgaatgcgtt aaagcattta cactcaggat gaatatatgg gaaaaattaa aattgtagtt
       61 tcagatcagc agccgtttat gattgatggg ataattggat tcctcggaca ttatcccgat
      121 ttatatgagg ttgttggggg ctataaagat ctgaagaaag ctatagcgga gtgtaacaaa
      181 tctgcagccc aaatatttat tttaggagaa tttgcaggtg gaatgatggg ggccgaactc
      241 gtaaaatggg ttaaatcgca taaaatagat gctcatatta ttacatttgt agcaaaaatg
      301 ccgtatattg attcaataaa attgcttgaa gcaggtgcga aaggatgcgt atggaaaacc
      361 agtcacccgg cgaaacttaa tcgcgctatt gattcgatta gtaacggcta cacttatttt
      421 gatagtgtac atatggattg tgagaaaata tcttccagat attcctctga taatcaactt
      481 acaaatcgtg agtctgaaat attacaactc atagctgatg gaaaaacaaa caaagaaatc
      541 gctaatttct tgcagttaag caggaaaaca gtcgaaactc atagactcaa tatcatgaag
      601 aaattagatg ttcacagtgg tatcgagttg atcaaaacag ctttacgtat gggtgtgtgc
      661 actatataaa aaaaagaggc tttaggctca ttttgctaaa gccttacgat tgtctagttc
      721 attaagcctg gtatatacaa tatatatttt tttggcatat tgcagtcgct ttttttggtt
      781 atccattgca aaacccgcat tataagtccc caggcattgc cacgtcattc cacaacggga
      841 aaaatgctta tataaaatct cggttcctat tttaatgttc aggcagggat tatctaacaa
//
LOCUS       DABSYT020000372          895 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.378, whole genome shotgun sequence.
ACCESSION   DABSYT020000372 DABSYT020000000
VERSION     DABSYT020000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.378"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..420)
                     /locus_tag="ID725_RS26985"
                     /old_locus_tag="pgaptmp_005397"
     CDS             complement(<1..420)
                     /locus_tag="ID725_RS26985"
                     /old_locus_tag="pgaptmp_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853756.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNININCPLKDIYNEFRRLN"
     gene            complement(442..>895)
                     /gene="yhaB"
                     /locus_tag="ID725_RS26990"
                     /old_locus_tag="pgaptmp_005398"
     CDS             complement(442..>895)
                     /gene="yhaB"
                     /locus_tag="ID725_RS26990"
                     /old_locus_tag="pgaptmp_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HGV7853757.1"
                     /translation="YWTVKRKFAEIVSTNDVNKIYSISNELRRVLSAITALNFYQGDV
                     PSVMIRIQPENMSPFIIDISTGEHDDYIIQTLDVGTFAPFGEKCTCSAVNKKELECIK
                     ETISKYCAKFTRKEAILTPPAYFNKTSITSDCWQILFFSPDHFNNDFY"
BASE COUNT          276 a          143 c          159 g          317 t
ORIGIN      
        1 gtttaacctc ctgaattcat tgtaaatatc ttttaatggg caattaatat tgatattacc
       61 tgtggatatt tcctggtttt ttaacggttc attaatggta agttcacaag tattgtccga
      121 tcgttgaata atttgggcgt tataacctaa aaaatccaga cgaagagacg agtcattata
      181 aagaattaca ttttgtaagt acgctaattc tttttgaaaa tcaccagtga taatgcattt
      241 tccactaggt tgtaacgtgt taagtaaagt gaccatttca atagaggtct gttttagttt
      301 ttcaatacct ggtgctagtg cttcgaggtt atttcctgtt gggctttcag gtaggtcatt
      361 catttttcga cggactaaat cactactaat atcattacca attgatgaaa cagggaacat
      421 tattttatcc tttataaata atcaataaaa atcattatta aaatgatcgg gtgaaaaaaa
      481 gagaatttgc caacaatctg aagtaatact ggttttatta aagtacgcag ggggtgttaa
      541 gatggcttct tttcgggtga attttgcaca atacttagaa atcgtctcct taatacattc
      601 cagctctttt ttattgacgg ctgagcaagt acatttttcg ccaaaaggtg caaaagtgcc
      661 tacatctaat gtttgtatga tataatcgtc atgttctcct gtagaaatat ctatgatgaa
      721 tggactcata ttttccggtt ggattcggat catgacagaa ggaacatcgc cttgatagaa
      781 attcaatgca gtaattgcag ataatactct ccgcagttca ttacttatac tgtaaatttt
      841 attaacgtca ttggtggaga caatttctgc aaactttctt tttaccgtcc aataa
//
LOCUS       DABSYT020000373          888 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.379, whole genome shotgun sequence.
ACCESSION   DABSYT020000373 DABSYT020000000
VERSION     DABSYT020000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.379"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            263..865
                     /gene="fimB"
                     /locus_tag="ID725_RS26995"
                     /old_locus_tag="pgaptmp_005399"
     CDS             263..865
                     /gene="fimB"
                     /locus_tag="ID725_RS26995"
                     /old_locus_tag="pgaptmp_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGV7853758.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          261 a          161 c          198 g          268 t
ORIGIN      
        1 attaatgatg agggttatta ttagattcgt atccgattga taaatatata aaggtacata
       61 gcatgcaaga gcatagcgtt tgtatggcaa cgttattata attaactgtt gctactccat
      121 ttaagttcac tcagaagaac tggtccactt acgttagtta ttaagcaaac gttcgctttt
      181 ataaacataa tcaggataaa aatgttggat tattgctaac ctagcacagc tagtgcgcgt
      241 ctgtaattat aagggaaaaa cgatgaagaa taaggctgat aacaaaaaaa ggaacttcct
      301 gacccatagt gaaatcgaat cactccttaa agcagcaaat accgggcctc atgcagcacg
      361 taattattgt ctgactttgc tttgttttat tcatggtttc cgggcgagtg aaatttgtcg
      421 attgaggatt tcggatattg atcttaaggc aaagtgtata tatatccatc gattaaaaaa
      481 aggcttttca acaacgcacc cgctattgaa caaagaagtt caggctttaa aaaactggtt
      541 gagtatccgt acttcttatc cgcatgctga gagcgagtgg gtatttttat cgcgtaaggg
      601 gaatccgctt tctcggcaac aattttacca tattatctcg acttccggtg gtaatgccgg
      661 gttgtcactg gagattcatc cgcacatgtt acgccattcg tgtggttttg ctttggcgaa
      721 tatgggaata gatacgcgac ttatccagga ttatcttggg catcgcaata ttcgtcatac
      781 tgtctggtat actgccagca atgcagggcg tttttacggc atctgggata gagccagagg
      841 acgacagcgt cacgctgttt tatagagata gttgagatac cagggatg
//
LOCUS       DABSYT020000374          886 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.380, whole genome shotgun sequence.
ACCESSION   DABSYT020000374 DABSYT020000000
VERSION     DABSYT020000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.380"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(27..245)
                     /gene="escE"
                     /locus_tag="ID725_RS27000"
                     /old_locus_tag="pgaptmp_005400"
     CDS             complement(27..245)
                     /gene="escE"
                     /locus_tag="ID725_RS27000"
                     /old_locus_tag="pgaptmp_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HGV7853759.1"
                     /translation="MITITELEDEIIKNKEAANVFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRTIEIMRIRINNK"
     gene            complement(260..631)
                     /gene="ler"
                     /locus_tag="ID725_RS27005"
                     /old_locus_tag="pgaptmp_005401"
     CDS             complement(260..631)
                     /gene="ler"
                     /locus_tag="ID725_RS27005"
                     /old_locus_tag="pgaptmp_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HGV7853760.1"
                     /translation="MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEEIIPLKNI"
BASE COUNT          247 a          177 c          121 g          341 t
ORIGIN      
        1 cttgtttggc tcacaatact catcctctat ttattattaa tcctgattcg cattatctct
       61 atagtcctaa ttgccgcatc acacgcaatg agcagttcct ttgcttcatt gtaggtaact
      121 ttatccaaag ggtgtttcat tttttcatgg atttcgttct ttttgtcgtt tattttttca
      181 ataaaaacat ttgcggcttc tttatttttt attatttcat cttccagctc agttatcgtt
      241 atcatttaat tatttcatgt taaatatttt tcagcggtat tatttcttct tcagtgtcct
      301 tcacaagaaa atcttctttc ttcattccat tcaacagtgc ttctttaagc cagcgtggct
      361 gtcggcctac gcccgaccag gtctgccctt cttcattgcg gtagtaaaca cctttcgatg
      421 agttccggcg agcgagtcca tcatcaggca cattagtata tcccagctct tgtaaggtta
      481 tattgtaata agtaatctgc ttctgaatcg actcaattac actttgaact tcctgctctc
      541 gcagtcgctt tgcttcctgc tgtagaactg caatttgctc tataagctga atgtatggac
      601 ttgttgtatg tgaattattt tccatattca taataaataa tctccgcatg ctttaatatt
      661 ttaagctatt agcgacctta tcaggaagga ccaacaatta atcaaatgat ataaattatg
      721 tgagataacg tttatctatc aaattaggac acatctattt catcaaacaa ccaccttaaa
      781 atgttattat tctctttttt ctaatgtgta aaatacatta tcattaaatg tcaacaaaaa
      841 aatcatatat ttttaaaatc catttaaaat caatgatata gaagaa
//
LOCUS       DABSYT020000375          850 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.381, whole genome shotgun sequence.
ACCESSION   DABSYT020000375 DABSYT020000000
VERSION     DABSYT020000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.381"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>850
                     /locus_tag="ID725_RS27010"
                     /old_locus_tag="pgaptmp_005402"
     CDS             <1..>850
                     /locus_tag="ID725_RS27010"
                     /old_locus_tag="pgaptmp_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853761.1"
                     /translation="QNSRDIRNIKNFSSHQHSLGDMFDELINIIDKEQVLSKEQRKVI
                     FRRYEDLYVKLMHYSVFTDKTHQIIKQKYFNDIVPMILALDIRNTYRPDNEMAFYYHI
                     HSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFIRNIRKNS
                     TPGKTKLIATINTCKETCKHLLYLSNEDKEKIISDLDKVQVACYYLTILLAFERRTSL
                     TSTLATLYKMLISEREVSEYECQLLYLTNPIDVMNILNKYIYYFPNENSPFYTLKIDS
                     ALSWDAI"
BASE COUNT          321 a          148 c          130 g          251 t
ORIGIN      
        1 cagaatagcc gtgacatccg aaatattaaa aatttttcct cacatcaaca ttcactgggc
       61 gacatgtttg atgaattaat aaacattatt gataaagaac aagtattgag taaagaacaa
      121 cgaaaagtta tatttaggag atatgaagat ctctatgtta agctaatgca ctattctgtt
      181 tttacagaca aaacacatca aataataaaa caaaaatatt ttaatgacat tgtaccaatg
      241 attctcgcac tcgacatcag gaacacatat cgcccggata atgagatggc attttactat
      301 catattcatt cttttctcac tcagataccg gataatgagg atgatatata tcatgctgca
      361 aggacatatc tgcgaaatta cgttaagtta tgtttatccg gatacacgcc agcgaatgcg
      421 catttcaaag atatctttga tggcgtatat gaattcattc gtaatattcg caaaaacagt
      481 acaccaggaa aaacaaaact tatcgcaact atcaacacat gcaaagaaac ctgtaaacat
      541 ctgctttatt taagtaatga agacaaggaa aaaataattt ctgacttaga taaagttcag
      601 gttgcatgtt attatctcac tatattactg gctttcgaaa gacgaacttc attaacaagc
      661 accctggcaa ctttatataa aatgctgata agcgaaagag aagtttcaga atatgaatgc
      721 cagttattat atttaaccaa cccaatagat gtaatgaata tactgaacaa atacatatat
      781 tactttccta atgagaactc accattttat acactgaaaa ttgacagtgc attatcgtgg
      841 gatgccattg
//
LOCUS       DABSYT020000376          842 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.382, whole genome shotgun sequence.
ACCESSION   DABSYT020000376 DABSYT020000000
VERSION     DABSYT020000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.382"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..193)
                     /locus_tag="ID725_RS27015"
                     /old_locus_tag="pgaptmp_005403"
     CDS             complement(<1..193)
                     /locus_tag="ID725_RS27015"
                     /old_locus_tag="pgaptmp_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGV7853762.1"
                     /translation="MLHDHLAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIK"
     gene            complement(719..>842)
                     /locus_tag="ID725_RS27020"
                     /old_locus_tag="pgaptmp_005404"
     CDS             complement(719..>842)
                     /locus_tag="ID725_RS27020"
                     /old_locus_tag="pgaptmp_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGV7853763.1"
                     /translation="STPTVQAPCVKPPSPPAWIMQPVPDWQKPLNGIISSSENG"
BASE COUNT          248 a          208 c          143 g          243 t
ORIGIN      
        1 gttttatttc ggtcaggttc accggtgaaa ccggagtccg gcgcgcctta cgcaaacact
       61 ctgctcgttt ctgtgccgcc acttttcttt tctggtcatc acttagctgt accatcactt
      121 ttgcccatcg ttcagctgct ctccggtaca gtcctttttt ctccagacat tctgccaggt
      181 gatcatgtag cataagtaac ctccgtttat ctacagactg ccatcctgaa tttaccgtcc
      241 ttaataagac aacaataaaa aactaattat ataaaaacaa taaaaataat atacaaaaaa
      301 actaaacaac aaagaaaagc aaatcatatc tggcatttta attgaaagag caattaccga
      361 acaaaagacg ctgactatat actcaaaacc aaacaacaat tctgccaatc aggtatcatg
      421 gcaacacacg gaattaccat gtttttgcct tctctgccca tacaatacgg gcatatactt
      481 catactctat tgtaatattt ctatccatgt gccccactcc atttacctgt aaataatatt
      541 caaaatattt atcacagaaa tcgtttttgg ccatgaactg agcacactat aaagtccgga
      601 actgactctt tgttaaatta ccttaacgtt accagtaaca ccttcgtaac aaaacatcac
      661 ggtatacact gggtacggat atattcctgt gctccttcca gttgcttctg cattgccatc
      721 agccgttctc tgaggatgaa ataatcccgt tcagcggctt ctgccagtcg gggaccggtt
      781 gcattatcca cgccggaggt gatgggggct ttacgcaagg agcctggaca gttggcgttg
      841 at
//
LOCUS       DABSYT020000377          837 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.383, whole genome shotgun sequence.
ACCESSION   DABSYT020000377 DABSYT020000000
VERSION     DABSYT020000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.383"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>837
                     /locus_tag="ID725_RS27025"
                     /old_locus_tag="pgaptmp_005405"
     CDS             <1..>837
                     /locus_tag="ID725_RS27025"
                     /old_locus_tag="pgaptmp_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGV7853764.1"
                     /translation="EFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEIT
                     QTVSKTLENQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDG
                     QPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTF
                     SLTPDGRLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSF
                     PRTSTYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVT
                     RNG"
BASE COUNT          241 a          198 c          234 g          164 t
ORIGIN      
        1 ggaatttttc cgggaaaaaa taggaaaact gcatctggct caggggctat gggagctgat
       61 agacaacagc cagcttgcgg atgagatggc ggagatgaag accaccatca cggaaacccg
      121 caatgaaatc acacagacgg tcagtaaaac gctggagaac cagagcgcca ctatacagca
      181 gatacagcgc gtgcagaagg acacaaatga tgacctggct gcgctgtaca tgctgaaggt
      241 tcaaaaaacg aaagacggca ttccctatgt ggccgggatt ggtgcaggga ttgaggatac
      301 tgatggccag ccactgagca acatactgct gctggctgac cgtatcgcga tgataaatcc
      361 ggagagcggc aacagcacgc cgttatttgt ggcgcagggg aatcagctgt tcatgaacga
      421 cgtgttcctg aagcgactgt ttgcggtgag tatcacctcg tccggcaatc ccccgacgtt
      481 ttccctgacg ccggacgggc gactgacggc gaaaaatgcg gatatcagtg gcagtgtgaa
      541 tgcgaactca gggacgctca acaacgtcac gattaatgag aactgtcaga ttaaggggaa
      601 actgtcagcc aaccagattg aaggcgatat tgtcaaaacg gtcagcaagt ctttcccccg
      661 cacgagcact tatgccagtg gcaccatcac ggtaagaatc agtgatgatc agaagtttga
      721 ccggcaggtc atgataccgc cagtgttatt ccgcggtggt aagcatgaga atttcaacag
      781 taataaccaa cagtcatact ggtattcaac ctgccggtta agagtgaccc gcaatgg
//
LOCUS       DABSYT020000378          834 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.384, whole genome shotgun sequence.
ACCESSION   DABSYT020000378 DABSYT020000000
VERSION     DABSYT020000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.384"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..661
                     /locus_tag="ID725_RS27030"
                     /old_locus_tag="pgaptmp_005406"
     CDS             <1..661
                     /locus_tag="ID725_RS27030"
                     /old_locus_tag="pgaptmp_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HGV7853765.1"
                     /translation="HKSLLFKTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFI
                     AEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIA
                     NPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATH
                     RRYDYVSFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDKYIEGEDTKSENNDNNLK
                     N"
     gene            complement(722..>834)
                     /locus_tag="ID725_RS27035"
                     /old_locus_tag="pgaptmp_005407"
     CDS             complement(722..>834)
                     /locus_tag="ID725_RS27035"
                     /old_locus_tag="pgaptmp_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HGV7853766.1"
                     /translation="LEEKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
BASE COUNT          299 a          130 c          150 g          255 t
ORIGIN      
        1 tcataaatca ctgcttttta aaactcttga tggagatatc atatctttat tatcagattg
       61 gggagagatg ctattccttc tcatactgta ttgtctcggc agtatgataa ttgtcttaat
      121 ttttgatttt atcgctgaat attttttatt tatgaaagat atgaaaatgg ataaacaaga
      181 agttaaaaga gaatacaagg aacaagaagg aaatcctgaa attaagtcta aacgcagaga
      241 gcgccatcag gaaattcttt ctgagcaatt gaaatctgat gtcagtaata gccgtttgat
      301 gattgccaac cctactcaca ttgcaatagg gatatacttt aagccacatc tgtcacctat
      361 tccattgatt tctgtaagag aaactaatga ggtagcatta gctgtaagga aatatgcaaa
      421 ggaaatcggg ataccaatta ttacagataa aaaattagca cgaaaaattt atgctaccca
      481 tcgtcgctac gattatgtta gcttcgaaaa tatagatgaa atattacgtc ttctgctgtg
      541 gcttgaagat gtggagaatg ctggacaacc tgttccagat gaacagctct cttcagaaga
      601 taaatatatt gagggtgaag acacaaaaag cgagaataat gacaataatt taaaaaatta
      661 aacatgtatc gtagtcaaaa atttataaga atctacacgc aaaaaacgtg tagattttta
      721 tttaatgagt taaacgatct tgcgaaatga caagcaattg atacatactc atctctttat
      781 aagctggaac tgtagtaacc tgtgcaaatt cgcgatatag cttttcctcc agtt
//
LOCUS       DABSYT020000379          829 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.385, whole genome shotgun sequence.
ACCESSION   DABSYT020000379 DABSYT020000000
VERSION     DABSYT020000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.385"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>829
                     /locus_tag="ID725_RS27040"
                     /old_locus_tag="pgaptmp_005408"
     CDS             <1..>829
                     /locus_tag="ID725_RS27040"
                     /old_locus_tag="pgaptmp_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGV7853767.1"
                     /translation="LIEMRLLYLALTKIDSRKPQPASEYTLFAKEYRDAFSLDSKNCY
                     EQLKSAASSLGSKPIVTYEWNETKKRIDAVKRFWFSSIRYGVGNSESDITLRFSDSVS
                     QYLYELKSEFTQMNLEHMVKLDTPFSFRLYSWLYKYKNLSRNKKESGVISTDPITIEW
                     MKERTGLTGKYPVYKDFKKRVLDPAVDIINANTNLSVTYEGIKSGKRIESVVFTYLVE
                     NETTHGKKMAVKPLRPRMPSRPRVVKGSLAEATWARNCLNVMRGYLVALKEYDHTLKL
                     "
BASE COUNT          283 a          124 c          172 g          250 t
ORIGIN      
        1 cattaattga gatgcgcctt ctgtatttgg cactcactaa gattgatagc cgtaaaccac
       61 agcctgcaag tgaatataca ctgtttgcaa aggaatatag agacgcattt tctttggatt
      121 cgaaaaattg ctatgagcag ttaaaatctg ccgcaagttc tttaggttca aaaccaatag
      181 ttacttatga gtggaatgaa accaaaaaaa gaattgatgc cgttaaacga ttttggtttt
      241 cttcaataag atatggtgtt ggaaatagtg aaagcgatat aaccttgcgt ttctctgatt
      301 ctgttagcca atatctttat gaactgaaaa gcgaatttac gcagatgaat cttgagcata
      361 tggtgaaact tgatacacca ttttcattta ggctttactc gtggttgtat aagtataaaa
      421 acctaagtag aaataaaaaa gagagtggtg ttatatcgac tgatccaata actatcgagt
      481 ggatgaaaga acgtacaggg ctaacaggaa aatatcctgt atataaggac tttaagaaaa
      541 gagttcttga tcctgctgtt gatattatta atgccaatac aaatttaagc gtaacatatg
      601 aaggtattaa atctggcaag aggatagaat ctgttgtatt cacatatctt gttgagaacg
      661 aaactacgca tggtaaaaaa atggcagtta agcctttgcg tcctagaatg ccatctaggc
      721 cacgggtagt taaaggaagt ctggctgaag ctacttgggc aagaaactgt cttaatgtta
      781 tgagagggta tcttgttgct ttaaaagaat atgatcatac attaaagct
//
LOCUS       DABSYT020000380          821 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.386, whole genome shotgun sequence.
ACCESSION   DABSYT020000380 DABSYT020000000
VERSION     DABSYT020000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.386"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            65..367
                     /locus_tag="ID725_RS27045"
                     /old_locus_tag="pgaptmp_005409"
     CDS             65..367
                     /locus_tag="ID725_RS27045"
                     /old_locus_tag="pgaptmp_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGV7853768.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            435..767
                     /gene="emrE"
                     /locus_tag="ID725_RS27050"
                     /old_locus_tag="pgaptmp_005410"
     CDS             435..767
                     /gene="emrE"
                     /locus_tag="ID725_RS27050"
                     /old_locus_tag="pgaptmp_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HGV7853769.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGLFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
BASE COUNT          229 a          145 c          215 g          232 t
ORIGIN      
        1 gtgcacaggc tctggcgaag atcgcagaaa ttaaagctaa gttcggactg aaaggagcaa
       61 gtgtatgacg ggcaaagagg caattattca ttacctgggg acgcataaga gcttctgtgc
      121 acaggacgtt gccgcggtaa caggcgcaac cgtaaccagc ataaatcagg ctgcggctaa
      181 aatggcgcgg gcaggaatcc tggtcgttga tggtaaggtc tggcgaacgg tgtattaccg
      241 gttcgctacc agagaagaac gggaaggaaa ggtgagcacg aatctgattt ttaaggagtg
      301 tcgccagagt gccgcgatga aacgggtatt gagggtatat aaaagaacat caatgggtac
      361 acaatgatga aacaggtgag ttgagttcaa actgtagtac aattctctcc agtttgaaca
      421 ggaaagaata ttctatgaac ccttatattt atcttggtgg tgcaatactt gcagaggtca
      481 ttggtacaac cttaatgaag ttttcagaag gttttacacg gttatggcca tctgttggta
      541 caattatttg ttattgtgca tcattctggt tattagctca gacgctggct tatattccta
      601 cagggattgc ttatgctatc tggtcaggag tcggtattgt cctgattagc ttactgtcat
      661 ggggactttt cggccaacgg ctggacctgc cagccattat aggcatgatg ttgatttgtg
      721 ccggtgtgtt ggttattaat ttattgtcac gaagcacacc acattaaaaa taatttgttt
      781 ctaaacgact aaaatatgga ggttcttata tttatatgag c
//
LOCUS       DABSYT020000381          807 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.387, whole genome shotgun sequence.
ACCESSION   DABSYT020000381 DABSYT020000000
VERSION     DABSYT020000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.387"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>807
                     /locus_tag="ID725_RS27055"
                     /old_locus_tag="pgaptmp_005411"
     CDS             <1..>807
                     /locus_tag="ID725_RS27055"
                     /old_locus_tag="pgaptmp_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGV7853770.1"
                     /translation="KGQYGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGAD
                     GTYSDASGASENSTRWGVDKPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTP
                     ANHAAQFGALVDKFRADLADMAGQCVGGSADGVPWICGDTTYFWKQKNEATYQTVYGS
                     YKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRAS
                     HFSAWARRGIISDRLATAILRHAGRVALNAGASSTVSEVRPSSPSGAEATG"
BASE COUNT          195 a          194 c          258 g          160 t
ORIGIN      
        1 aaggggcagt acggaaccgt ggggcagggg ctgcatatcg ccaaaaaatt gctgccgttt
       61 ataccggcga atgcgggcat tctgctggtt ccgtgctgtc gtggtggttc agcgttcacc
      121 accggagctg atggcacata cagtgacgcg agtggtgctt cggagaattc aacccgctgg
      181 ggtgtggaca agccgctgta taaggacctt atcggtcgaa caaaagcagc actgaagaag
      241 aacccgaaaa atgtgctgtt tgccgtggtg tggatgcagg gggaatttga ttttggcggt
      301 acgccggcaa atcacgcagc acagtttggt gcgctggttg ataaattccg tgcagacctg
      361 gcggatatgg caggtcagtg cgtcggtggc tctgctgacg gtgttccctg gatatgcggg
      421 gacacgacgt atttctggaa gcagaagaac gaagccacct accagacggt gtacggcagc
      481 tacaaaaaca aaacggaaaa aaatatccat ttcgtaccgt tcatgacgga tgagaacggg
      541 gtgaatgtgc cgacgaacaa accggaagaa gacccggaca ttccgggtat cggatattac
      601 ggttcgaaat ggcgtgacag ctcagccacc tggacgtcac aggacagggc gagccatttc
      661 agcgcctggg cacgccgtgg gattatttcc gaccgtctgg caacggcgat tttgcgccat
      721 gcgggaagag tggcgctaaa cgcgggggca tcatcgacag tatcagaggt gcgcccgtca
      781 tcgccttccg gtgcagaagc cacaggc
//
LOCUS       DABSYT020000382          801 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.388, whole genome shotgun sequence.
ACCESSION   DABSYT020000382 DABSYT020000000
VERSION     DABSYT020000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.388"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..357
                     /locus_tag="ID725_RS27060"
                     /old_locus_tag="pgaptmp_005412"
     CDS             <1..357
                     /locus_tag="ID725_RS27060"
                     /old_locus_tag="pgaptmp_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGV7853771.1"
                     /translation="AAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGV
                     AQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDIST
                     RDEGGDGKVVVIGRQR"
     gene            596..>801
                     /locus_tag="ID725_RS27065"
                     /old_locus_tag="pgaptmp_005413"
     CDS             596..>801
                     /locus_tag="ID725_RS27065"
                     /old_locus_tag="pgaptmp_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853772.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWR"
BASE COUNT          204 a          170 c          254 g          173 t
ORIGIN      
        1 gcggccatcg tggggtcgtt cttcactgcc ggggcatcaa tggcgttatg gggttcagcc
       61 ctggcagccg gtggtttttc tgccaccacg atgctgtttt cactgggggc cagcatgata
      121 ctgggtggtg tggctcagat gctggccccg aaggctaaag taccggagta caaaagcacg
      181 gataacggca gacagaacac gtacttttcc tcgctggata acatgattgc acaggggaac
      241 ccgatgccgg tgccttacgg tgaaatgctg gttggctcac ggcgaatctc ccaggacatc
      301 agtacccgtg atgaaggcgg tgacgggaag gtggtggtta tcgggcggca gaggtaaaaa
      361 gaataaaaaa atcccgcagt gaccgaaggc tgcgggaaca gaaaatgaag attaaccaca
      421 gggagttttg tttttattgg cccgaaaaaa ctgtaacgcc cgggaatgat atctgccacg
      481 ggggcgtaca gaaaatgtga agatattcag aatttttatt cagtcatgat acaggcatcc
      541 tccgggatgc ctgttgtttt tgtgcgtaac agttatcaca gtaaagggtg agacaatggg
      601 caaaggtggt ggcaaggcgc acacgccggt tgaggcaaag gacaatctta agtccacgca
      661 gatgatgagc gtgattgatg cgattggtga agggccgatt gaaggtccgg tgaaggggct
      721 gcagagtatc ctggtgaaca aaaccccgct gacggacacg gacggtaatc ctgtgataca
      781 tggtgtgaca gcggtctggc g
//
LOCUS       DABSYT020000383          793 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.389, whole genome shotgun sequence.
ACCESSION   DABSYT020000383 DABSYT020000000
VERSION     DABSYT020000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.389"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(24..>793)
                     /locus_tag="ID725_RS27070"
                     /old_locus_tag="pgaptmp_005414"
     CDS             complement(24..>793)
                     /locus_tag="ID725_RS27070"
                     /old_locus_tag="pgaptmp_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853773.1"
                     /translation="SFDDSTRRLELEWHPNDKFHIYIESVVFVEFNDTIYAFLKGYYH
                     NQLSLNRIYNTKCSSCSFENSGSYMVDVYRTVKRVNETIQTPNINCYTTCILTRPANG
                     HVTSTGFSRNLMDAINISLAEHKIASLHNEVMSIEEYDRDSLQEKGNTARRYLEYILM
                     LVNIRIMHLNNVQYQEQMLGSLVSVIEALDYEPLMKNDVEITKDILNACSHHGGVRIE
                     KKDVIFSLEVIENLIKAIKKTDINKLQLDGMFKSIQK"
BASE COUNT          245 a          134 c          123 g          291 t
ORIGIN      
        1 tctaattcat tgttagatga tgtttatttt tgaattgatt taaacatccc atcgagttgt
       61 aatttattta tgtcggtttt ctttatggct ttaattaagt tttcaataac ttccaaagaa
      121 aaaattacat ctttcttttc aattctaacc cccccgtggt ggctgcaagc attaaggatg
      181 tcctttgtaa tttcaacatc atttttcatt aatggttcat agtccagagc ctcaataaca
      241 gaaactaaac tacctagcat ttgctcttgg tattgaacgt tgtttaaatg cattatacga
      301 atattaacaa gcatgagaat atattctaaa tatctcctgg cagtatttcc tttctcttgt
      361 aaagaatcac gatcatattc ctcaatactc atgacttcat tatgtaaaga ggctattttg
      421 tgctctgcta atgaaatatt tatcgcatcc atgaggtttc gtgagaagcc tgtagatgta
      481 acatgaccat ttgcgggcct tgttaaaata catgtagtat agcaatttat atttggggtc
      541 tggattgtct catttactct tttaaccgtt ctataaacgt ctaccatata agaacctgag
      601 ttttcaaatg aacatgaaga gcattttgta ttatatattc tatttagaga taactgattg
      661 tgatagtatc cctttaagaa agcatatata gtgtcattaa actcgacaaa cacaacggac
      721 tcaatatata tatggaattt atcattgggg tgccactcta attcaagtct tcttgtagag
      781 tcatcaaagg aaa
//
LOCUS       DABSYT020000384          762 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.390, whole genome shotgun sequence.
ACCESSION   DABSYT020000384 DABSYT020000000
VERSION     DABSYT020000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.390"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>762
                     /locus_tag="ID725_RS27075"
                     /old_locus_tag="pgaptmp_005415"
     CDS             <1..>762
                     /locus_tag="ID725_RS27075"
                     /old_locus_tag="pgaptmp_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HGV7853774.1"
                     /translation="SGNWKTSFLCNLARLLYSIFNGSNYFCSREGENNSSPSSTLLTI
                     HQPEKQELLQQKSIKHLPTSNNIDGYIKIRKTRGAEDQTTTITQSLIINELLNGVDRN
                     TIPFQKISELNDIIHSYENMQIKNSRKGIEILVKQGELLSSLINDNKGNKQLSDNASK
                     IINLLGIEYQSHKVDIEPFIHAVWVAGAPPDNTFSYITAFLNTYKDYTYLLWIDPNAF
                     GAAKFSGILKNIAMNYAIMRLRRTNPHLAEEMNEV"
BASE COUNT          298 a          117 c          128 g          219 t
ORIGIN      
        1 actcaggaaa ctggaaaaca tcttttttat gtaatcttgc tagactacta tatagcatat
       61 ttaatggtag taactatttt tgttcccgag agggtgaaaa taattcatcc cccagttcta
      121 ctctacttac tatacatcag cctgaaaagc aggaactatt acaacaaaag agtatcaaac
      181 atttaccaac aagtaataac atcgacggat acattaaaat aagaaaaaca agaggcgctg
      241 aagatcaaac aacaactatc actcaaagtt tgataattaa tgagttgtta aatggagttg
      301 atagaaatac catccctttt cagaaaataa gtgagctcaa tgatatcata cattcatatg
      361 aaaatatgca aattaaaaat agtcgaaaag gtatagaaat acttgttaag cagggagagc
      421 tgttatcatc attaataaat gataataaag gaaataaaca attatcagac aatgcatcta
      481 aaataataaa cttattgggt atagagtatc agtcacataa agtagacata gagccattta
      541 tacatgcagt atgggttgct ggcgcacctc ctgataatac attttcatat ataacggcat
      601 ttttaaatac atataaagat tatacctacc tgctctggat tgatcctaat gcctttggtg
      661 cggccaagtt tagtgggatt ttaaaaaata tagcgatgaa ttatgcaatt atgcgtttga
      721 gaagaacgaa tccacattta gcggaagaaa tgaatgaagt ga
//
LOCUS       DABSYT020000385          756 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.391, whole genome shotgun sequence.
ACCESSION   DABSYT020000385 DABSYT020000000
VERSION     DABSYT020000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.391"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>756
                     /locus_tag="ID725_RS27080"
                     /old_locus_tag="pgaptmp_005416"
     CDS             <1..>756
                     /locus_tag="ID725_RS27080"
                     /old_locus_tag="pgaptmp_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGV7853775.1"
                     /translation="IHVFSRYLKWLCGLVHSSKGIHAKYEVDVFIESIRAHIPRNSSL
                     NMNEISEKSLNEEEIKVLFRLLEIGGIENPFHKEVQVRNRLIFTLLLNLGLRAGELLN
                     LKIDDFDLRDNTLSVVRRHDSKEDGRSYQPLVKTGERVIPLSDELAREIFDYISNSRE
                     KMTKRKKHNFLFVAHCTGKNAGEPLSISAYEKVISTLKRASPELYNLSGHRLRHSWNY
                     MYSKGIEGAELEYNRRKDLRNYLMGWSKESIMCD"
BASE COUNT          274 a          102 c          163 g          217 t
ORIGIN      
        1 atacacgttt tttcacgcta ccttaagtgg ttatgtggat tagttcattc gtcaaagggg
       61 attcatgcaa agtatgaagt agacgtattt attgaaagta tcagagcaca tattccaaga
      121 aacagttcat tgaatatgaa tgaaatatct gaaaaatcgt tgaatgaaga agagatcaaa
      181 gtgcttttcc gtctattgga aattggcggg attgaaaacc ctttccataa ggaagtacag
      241 gtcaggaaca gactaatatt taccttgctc ttaaatttag gtttaagggc tggagagttg
      301 ctcaatctta aaatagatga ttttgatctc agagacaaca ctttaagtgt agtcagaaga
      361 catgattcaa aagaagatgg aagatcttat caaccattag taaagactgg agaaagggtt
      421 attccgttat ctgatgagtt agcccgtgaa atttttgatt acataagcaa tagcagagaa
      481 aaaatgacaa aaaggaaaaa gcataatttc ttatttgtgg ctcattgcac cggaaaaaac
      541 gcaggagaac ccttaagcat ttcagcttat gagaaggtga tatcaacact gaaacgagca
      601 agtccagagc tatataatct ttcaggccat aggttaaggc actcttggaa ttatatgtat
      661 tctaaaggaa tagaaggtgc tgaattagaa tataatagaa ggaaagattt aaggaattat
      721 ttaatgggtt ggtcaaagga gtcaataatg tgtgat
//
LOCUS       DABSYT020000386          755 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.392, whole genome shotgun sequence.
ACCESSION   DABSYT020000386 DABSYT020000000
VERSION     DABSYT020000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.392"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            40..>755
                     /locus_tag="ID725_RS27085"
                     /old_locus_tag="pgaptmp_005417"
     CDS             40..>755
                     /locus_tag="ID725_RS27085"
                     /old_locus_tag="pgaptmp_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGV7853776.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          250 a          133 c          164 g          208 t
ORIGIN      
        1 taccagtaat gtaaataaat tttcgaggag atcattcctg tgggacgtaa atgggccaat
       61 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
      121 gtagaaatct atgctgccgc taaacaaggt gaacccgatc cagaattaaa cacatcttta
      181 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
      241 attgataaag ccaaaggcgg cggagatgaa acattcgtgc agggccgtta tgaaggcttt
      301 ggtcctaatg gctcaatgat tatcgccgag acattgactt caaatgttaa ccgtacgatt
      361 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
      421 agctatatgt ttgacaatac tggtgtgatt gtatttaaag gatcagaccc tgaccatatt
      481 tttgaaattt tacttgaagc tgaagttgat gttcgtgatg tgactgaaga agaaggtaac
      541 attgttattt ataccgaacc tactgacctt cataaaggaa tcgcggctct aaaagcagct
      601 ggaatcagtg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
      661 tcaccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
      721 gtacaaaaag tttatcataa cgtcgcaaat ctcta
//
LOCUS       DABSYT020000387          745 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.393, whole genome shotgun sequence.
ACCESSION   DABSYT020000387 DABSYT020000000
VERSION     DABSYT020000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.393"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..655)
                     /gene="ytfI"
                     /locus_tag="ID725_RS27090"
                     /old_locus_tag="pgaptmp_005418"
     CDS             complement(<1..655)
                     /gene="ytfI"
                     /locus_tag="ID725_RS27090"
                     /old_locus_tag="pgaptmp_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HGV7853777.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIHHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLDDIVSKLKRGKFERQGPGGGIKQCDGWYIYEKIIDDNFAIVIESLG
                     "
BASE COUNT          231 a          150 c          103 g          261 t
ORIGIN      
        1 agcccaaaga ttctatgact atggcaaaat tatcatcaat tattttttcg taaatatacc
       61 atccatcgca ctgtttaatt ccacctcctg gcccttgtcg ttcaaattta ccccgtttta
      121 atttgcttac tatatcatcc aataaagcat aacctaaaga cacgtcacca atcgcctggt
      181 gaatataacc tttaatcctt ttcggtatgt cagactgttt tttctgcaga atatcccagg
      241 catacaatcg tctgttttgt tctaacggaa attcacggtt cttgaaatcc atgaatgagt
      301 tatactcatt tattgttatg gggatttctt cgtattcaaa tccactgcct cgcaatgtca
      361 ttgtatattc ggcaccgtta tctttaaggc tgaaactgat aagaaactta tccatagcgt
      421 taacagtttc attttttgat gaaagcacct gataactact tttatcaatc tgagttgcgc
      481 aacgaaagaa ctccctcaaa gttaacaggc tatcataatt cgaaatccaa tcttttttct
      541 taaatgcatg atggatgttg ttaaaaaaat tgtttgaatg tgtttttgta tacgaagact
      601 ttacaaataa ctccaccgca ccaataaaat tattgtgtct gatcctgggt aacatatgac
      661 aaccttattt tatttggtta ctttatatcg ccattaaata tctcactata gaagtaaact
      721 atcaaaaaac gctcattatt aaaat
//
LOCUS       DABSYT020000388          738 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.394, whole genome shotgun sequence.
ACCESSION   DABSYT020000388 DABSYT020000000
VERSION     DABSYT020000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.394"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            141..710
                     /locus_tag="ID725_RS27095"
                     /old_locus_tag="pgaptmp_005419"
     CDS             141..710
                     /locus_tag="ID725_RS27095"
                     /old_locus_tag="pgaptmp_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGV7853778.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
BASE COUNT          211 a          154 c          191 g          182 t
ORIGIN      
        1 gtcatttctg cgcatctcac gcgcatctca caacacagaa cctttcagga tgacccttga
       61 ggataccggt ttggctgtcg gtgcctttct gtgggctgga ttcctgtgag acaaggttca
      121 tcactaaaag gaaataaccg atgaatatga tggccgtgcc gtttcacggc aactctcttt
      181 atgtagttaa ccataatggc gaaccatacg ttcccatgaa acctgtcgtt gcggggatgg
      241 ggctggcctg gcaatcacag ttggctaagt taagacagcg ttttgcgtca actataacgg
      301 aaatcgttat ggttgctgag gatgggaaac aacgcaatat ggtgtccatg ccacttcgaa
      361 aacttgccgg ctggctacaa accattaatc ccaacaaagt aaaacccgaa atccgcgata
      421 aggtcatccg gtatcaggaa gagtgcgacg atgttcttta cgagtactgg acgaagggtt
      481 ttgtcgttaa tccccgtaaa atgagcgtga tggaagaact caaccaggct tgtgctgaca
      541 tgaaacggga taaaaacatt gccagtgtgt ttgctaccgg gctgaatgag tggaaacagg
      601 ttaaagccgc gcatgtatca aaaatccgta cgctggtaaa tgaagcgaat atgctgattg
      661 attttgtcct ggctgataca ggcaaaggga aaataacaaa ggcggattga tggggtggct
      721 aatgatatca gataaact
//
LOCUS       DABSYT020000389          738 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.395, whole genome shotgun sequence.
ACCESSION   DABSYT020000389 DABSYT020000000
VERSION     DABSYT020000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.395"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..280
                     /locus_tag="ID725_RS27100"
                     /old_locus_tag="pgaptmp_005420"
     CDS             <1..280
                     /locus_tag="ID725_RS27100"
                     /old_locus_tag="pgaptmp_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGV7853779.1"
                     /translation="IRYRSKYYSRDSALIEEETIFSDAELKAILPMRRMYGVSDYKSN
                     SSSLSSHSGLKDPTGTPVCYYIHNESKPSLGYGPTSNNWLSQSFTTEL"
     gene            343..>738
                     /gene="espR1"
                     /locus_tag="ID725_RS27105"
                     /old_locus_tag="pgaptmp_005421"
     CDS             343..>738
                     /gene="espR1"
                     /locus_tag="ID725_RS27105"
                     /old_locus_tag="pgaptmp_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HGV7853780.1"
                     /translation="MKFPSIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLSTQPPGLQNFTSINLDNNQLTHFDATNY
                     DRLVKLSLNSNTLESINIHQGRNVSITHIS"
BASE COUNT          269 a          153 c          112 g          204 t
ORIGIN      
        1 tattcgttat agatcaaaat attattccag agattccgcc ttaatagaag aagaaacaat
       61 attttctgac gcagaactaa aagctattct gcctatgcgt cgcatgtacg gggttagtga
      121 ctataagtca aattcctctt ctctatcctc acactcggga ctcaaggacc caacgggcac
      181 acccgtctgt tattatattc ataatgagag taaaccttcc ttaggttatg gtccaacatc
      241 caataattgg ttaagccaat cctttacaac agagttataa aacacatcct ggtttatcaa
      301 gggtgataaa tcagaaaaac attgtattac tatggagcag ttatgaaatt cccttcaata
      361 tttaacaaaa taaaaccaca atccatacag caacatccag aaaaaaatca acttaactgg
      421 atgctcgaat taaataaatg gaaagaagaa cgtatactta caggtgaaat ccatcgtccg
      481 gaatgtcgaa acgaagccgc taaaaggata aactgtgctt ttttgtcgaa acagaatgac
      541 attgatttat caggacttaa tttatctact caaccaccag ggctgcaaaa cttcacctct
      601 atcaatcttg ataataacca actcacacat tttgatgcaa ccaactacga tagactcgta
      661 aaacttagtc tgaatagtaa cactcttgag tcaataaata ttcatcaagg cagaaatgta
      721 agcattacac atatatct
//
LOCUS       DABSYT020000390          734 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.396, whole genome shotgun sequence.
ACCESSION   DABSYT020000390 DABSYT020000000
VERSION     DABSYT020000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.396"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(1..734)
                     /locus_tag="ID725_RS27110"
                     /old_locus_tag="pgaptmp_005422"
                     /pseudo
     CDS             complement(1..734)
                     /locus_tag="ID725_RS27110"
                     /old_locus_tag="pgaptmp_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369291.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          246 a           92 c          125 g          271 t
ORIGIN      
        1 tattaaaagt gaagatgata ttacgtttat tacaactgga gaaccaatat tttagagtat
       61 tattcgtcca ctttgttggt gtcttaatta gttgcgctgt tgttgttttt acattgttaa
      121 atgtagaacc gctaaaattt gtcccgacgc acgaaatgaa attcatctct gaattgttaa
      181 aattgcaatt ctttatctca cacatatcaa ggttaatatc atgaaatgtc gaatatttca
      241 tgttttcatg tgaaaaaaaa catgcaataa acgttatata ttgcaacctg gcattacaaa
      301 aagatttatt tttaaaatca aaacaaaagg tttagtttat cttgaaattg ttattatatc
      361 tgcaatgtaa agaaatcatg ttttttatta actgataaca ccattgtgat gattctggta
      421 attcataggg atgtaaatgg atgttttcat cgaatataac ctgtgagtta tggttgttta
      481 ttgaagatat atggaagcta tcatttgtta aagctatggt tgtgctaaaa ttatagtggt
      541 gatcagcgct catgaaatca aatgtaacaa tgtcgttgtt tcctttcttt tctttgtgat
      601 atgcgtttaa aaaagaaata ttttcgatct gttcatcagt taatgtaaat ggcatttcgt
      661 catcaaagaa agcgtaatcg gcatttggga gaggaatact ctcaattgag catagtttta
      721 ataaaaaaac atga
//
LOCUS       DABSYT020000391          732 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.397, whole genome shotgun sequence.
ACCESSION   DABSYT020000391 DABSYT020000000
VERSION     DABSYT020000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.397"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            506..694
                     /locus_tag="ID725_RS27115"
                     /old_locus_tag="pgaptmp_005423"
                     /pseudo
     CDS             506..694
                     /locus_tag="ID725_RS27115"
                     /old_locus_tag="pgaptmp_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          216 a          142 c          155 g          219 t
ORIGIN      
        1 ctactgatgt acaaaacaca acaatagctt catgatatat aaatacgtga tgacgagtgg
       61 tactccgcat ggttatttaa cttctaagca ccactaatga tcctgagcaa cttcgtattt
      121 gggccattac catggagtag aaagctgtgt ctgagaatgc agaatttgag aaaagaattc
      181 gctttcttgg agaaattctg aaaatggcac gctaacaacg ttttgttaaa aaatgtgaat
      241 tacttgccgg ggctgcattt tctattcgag gagaatgttg acgtagatat tacagaaatc
      301 tcagcgcatc aggataaact gctaccttag gtcaaccacc aaaagaaaat acaatacgtc
      361 cagtttgtat gcctttaata acacatcttc taaatgttga agaattatcc cgcctgctga
      421 agaacgttgc tctgagtgta ataaaccttt ttattcttga tgatgtcagc gagaagatgg
      481 aatatatccc cgactgggtc gtggttaccc ggttaaacat ctggtggtgc taataaatct
      541 ggcaagtcac attctgttga atgctgtgac agtgccttac cgagagagcg aaattgtggt
      601 gttctagtgc atagtcgcca ccattccgga taattctatt acgttgtttt acaagttctt
      661 ttacagcgca gatctgtcgc tgacgttgaa tcaatagcct ggatttcaga acacaatgcc
      721 cgtttgctgg tt
//
LOCUS       DABSYT020000392          720 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.398, whole genome shotgun sequence.
ACCESSION   DABSYT020000392 DABSYT020000000
VERSION     DABSYT020000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.398"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..126)
                     /locus_tag="ID725_RS27120"
                     /old_locus_tag="pgaptmp_005424"
     CDS             complement(<1..126)
                     /locus_tag="ID725_RS27120"
                     /old_locus_tag="pgaptmp_005424"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGV7853781.1"
                     /translation="MKISVITVTYNNAEGLEKTLSSLSILKIKPFEIIIVDGGSTD"
     gene            complement(95..>720)
                     /gene="wzy"
                     /locus_tag="ID725_RS27125"
                     /old_locus_tag="pgaptmp_005425"
     CDS             complement(95..>720)
                     /gene="wzy"
                     /locus_tag="ID725_RS27125"
                     /old_locus_tag="pgaptmp_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O157 family O-antigen polymerase"
                     /protein_id="HGV7853782.1"
                     /translation="RIIGYFLLVGVVCSLFYLSLGQDGEQNDSYNNMLRIINRLTIEQ
                     VESVPYVVSESIKNDFFPTPELEKELKAIINRIQGIKHQDLFYGERLHKQVFGDMGAN
                     FLSVTTYGAELLVFFGFLCVFIIPLGIYIPFYLLKRMKKTHSSINCAFYSYIIMILLQ
                     YLVAGNASAFFFGPFLSVLIMCTPLILLHDTLKRLSRNENISYNCDL"
BASE COUNT          256 a          131 c           90 g          243 t
ORIGIN      
        1 atctgtagag ccgccatcaa ctataataat ctcaaaaggt tttattttta aaattgataa
       61 actacttaaa gttttttcta acccttcagc attattataa gtcacagtta taactgatat
      121 tttcatttcg tgataatctc tttaacgtat catgcaataa gatcagagga gtacacatta
      181 tcaatacgga gagaaaagga ccaaaaaaga aggccgatgc attcccagcc actaagtatt
      241 gcaataaaat cataatgata tatgaataga atgcgcaatt tatcgagcta tgggtttttt
      301 tcattctctt taaaagataa aaaggtatat atatccctaa agggataatg aatacacaga
      361 gaaaaccaaa aaaaactaac agttctgctc catacgtagt aactgataaa aaatttgctc
      421 ccatgtctcc aaatacttgt ttatgtaacc gttctccata aaataagtct tgatgcttta
      481 ttccctgtat tctatttatt attgctttta attccttttc taactctggt gtcggaaaga
      541 aatcgttctt aatagattca gaaacaacat atggaacact ttcaacttgc tctattgtta
      601 acctattaat tatccttaac atattattat atgagtcatt ttgttctccg tcttgtccta
      661 aacttagata aaacaacgag catacaaccc ctaccaataa aaaataccct attattcttt
//
LOCUS       DABSYT020000393          714 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.399, whole genome shotgun sequence.
ACCESSION   DABSYT020000393 DABSYT020000000
VERSION     DABSYT020000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.399"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            116..607
                     /locus_tag="ID725_RS27130"
                     /old_locus_tag="pgaptmp_005426"
     CDS             116..607
                     /locus_tag="ID725_RS27130"
                     /old_locus_tag="pgaptmp_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853783.1"
                     /translation="MKPRNINNSLPLQPLVPEQENKNKKNEEKSVNPDKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFPITDDLMSMAVNELIKLT
                     KIENNVDLNKFTTICTDVLSPLVTKHNKQKSQNDILPFAKIPFLIFIEKMEIDNMNPG
                     HVH"
BASE COUNT          295 a          100 c          118 g          201 t
ORIGIN      
        1 aaaaaagtaa aaatgagcga tttttgatag aataaagcat gagaaaaggt gatatttgac
       61 attatcagaa gctgcgaatt cggattttgc tctaatcaaa tgagagaaat gtaaaatgaa
      121 gccacgaaat attaataata gcctaccact gcaaccatta gttcctgaac aggagaacaa
      181 aaataagaaa aatgaagaga aatccgttaa tccagataaa attacaatgg ggtctggttt
      241 aaattatatt gaacaagaat ctcttggagg gaaatatcta acacatgatt tgtcaataaa
      301 gatagcggat atttctgaag agataattca gcaagcaata ttatctgcta tgagcatata
      361 taaatttccg ataacagatg atttaatgag tatggctgta aatgaactca taaaactgac
      421 caaaatagag aataatgtag acctgaataa attcactact atatgcacag acgttctatc
      481 ccccctcgtc accaaacata ataaacaaaa aagtcaaaac gacattctac ccttcgcaaa
      541 aatccccttt ttaattttca ttgaaaaaat ggaaattgat aatatgaatc caggtcatgt
      601 acattaaggc attttgaaat gtcataatta ggataactaa taaggtgaat attagtataa
      661 cagctgatga tgttattaat ttgtagtgga aagtatttta actaataatg aaga
//
LOCUS       DABSYT020000394          700 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.400, whole genome shotgun sequence.
ACCESSION   DABSYT020000394 DABSYT020000000
VERSION     DABSYT020000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.400"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..426
                     /locus_tag="ID725_RS27135"
                     /old_locus_tag="pgaptmp_005427"
     CDS             <1..426
                     /locus_tag="ID725_RS27135"
                     /old_locus_tag="pgaptmp_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HGV7853784.1"
                     /translation="NDARTLFEFTSGVNVIESPIIYRDESMRTACSPDGLCSDGNGLE
                     LKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMWVTRKDAWYFANYDPRMKREGLHYV
                     VVERDEKYMASFDEMVPEFIEKMDEALAEIGFVFGEQWR"
     gene            423..605
                     /locus_tag="ID725_RS27140"
                     /old_locus_tag="pgaptmp_005428"
     CDS             423..605
                     /locus_tag="ID725_RS27140"
                     /old_locus_tag="pgaptmp_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGV7853785.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            578..>700
                     /locus_tag="ID725_RS27145"
                     /old_locus_tag="pgaptmp_005429"
     CDS             578..>700
                     /locus_tag="ID725_RS27145"
                     /old_locus_tag="pgaptmp_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGV7853786.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLA"
BASE COUNT          180 a          155 c          193 g          172 t
ORIGIN      
        1 aacgacgcca gaaccctgtt tgagttcact tccggcgtga atgttattga atccccgatc
       61 atctatcgcg acgaaagtat gcgtaccgcc tgctctcccg atggtttatg cagtgacggc
      121 aatggccttg agctgaaatg cccgtttacc tcccgggatt tcatgaagtt ccggctcggt
      181 ggtttcgagg ccataaagtc ggcttacatg gcccaggtgc agtacagcat gtgggtgaca
      241 cgaaaagatg cctggtactt tgccaactat gacccacgta tgaagcgtga aggactgcat
      301 tatgtcgtgg ttgagcggga tgaaaagtac atggcgagtt ttgacgagat ggtgccggag
      361 ttcatcgaaa aaatggacga ggcactggct gaaattggtt ttgtatttgg ggagcaatgg
      421 cgatgaagca tcctcacgat aatatccggg taggtgcgat cactttcgtc tactccgtta
      481 caaagcgagg ctgggtattt cccggccttt ctgttatcag aaatcccctg aaagcacagc
      541 ggctggctga ggagataaat aataaacggg gagctgtatg cacaaagcat ctcccgttga
      601 gttaagaacg agtatcggga tggcacatag cctcgctcaa attggagtca ggtttgtgcc
      661 aataccagta gaaacagacg aagaatttca tacgttagcc
//
LOCUS       DABSYT020000395          694 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.401, whole genome shotgun sequence.
ACCESSION   DABSYT020000395 DABSYT020000000
VERSION     DABSYT020000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.401"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            98..427
                     /locus_tag="ID725_RS27150"
                     /old_locus_tag="pgaptmp_005430"
     CDS             98..427
                     /locus_tag="ID725_RS27150"
                     /old_locus_tag="pgaptmp_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853787.1"
                     /translation="METLTFNNGTVSVGDVFVSSWGYEQTNVNFYQVISVHGKKTVTV
                     QEIRASVLLTRSMSGYKTPLLNDFCGEPLKRRVRDCYSVPAIEIESFEMAYKTQPEEK
                     HEFTSYY"
     gene            485..>694
                     /locus_tag="ID725_RS27155"
                     /old_locus_tag="pgaptmp_005431"
     CDS             485..>694
                     /locus_tag="ID725_RS27155"
                     /old_locus_tag="pgaptmp_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HGV7853788.1"
                     /translation="MHFTTFLKKHFDIEKIVGTSDSGNDTESIYVYEKGNDCESLFIL
                     RESWINAEIKKSGIWTVGDIYSTLEH"
BASE COUNT          217 a          125 c          152 g          200 t
ORIGIN      
        1 aaattacttg ccaataggtc agaactgacc tattataaac atatcgggag gggatagtcc
       61 ccgaccacca aagccgctca gcggcaggag cttgaaagtg gaaacattaa cttttaataa
      121 cggcactgtt tctgttggtg atgtgtttgt atcttcatgg ggatatgagc aaactaacgt
      181 taatttctac caggttattt ctgttcacgg taaaaaaacc gtcaccgtcc aggagattcg
      241 cgcttctgtt cttctgactc gttcaatgag tggatataag accccattgc ttaatgattt
      301 ctgtggggag ccattaaagc gtcgtgtgcg tgattgttac agcgtacctg caatagagat
      361 tgaatcattt gagatggcct ataaaactca gccagaagag aaacacgaat ttacatcata
      421 ttactaatca caggggcgta attgccccta attaccaaag ccgcacagcg gcaggagaat
      481 aaaaatgcat tttacaactt ttctgaaaaa acatttcgat atagaaaaaa ttgttggcac
      541 ttctgattct ggaaatgata ccgaatctat ttatgtttat gaaaaaggaa atgattgcga
      601 atcgttgttt attctgcgtg agtcatggat aaatgcagaa attaagaaga gtgggatatg
      661 gactgttggt gacatatatt ccacgttgga acat
//
LOCUS       DABSYT020000396          687 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.402, whole genome shotgun sequence.
ACCESSION   DABSYT020000396 DABSYT020000000
VERSION     DABSYT020000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.402"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..>687)
                     /gene="wzx"
                     /locus_tag="ID725_RS27160"
                     /old_locus_tag="pgaptmp_005432"
     CDS             complement(<1..>687)
                     /gene="wzx"
                     /locus_tag="ID725_RS27160"
                     /old_locus_tag="pgaptmp_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HGV7853789.1"
                     /translation="LSIITLVISSKLHAGLPVLIVSTLGIQYISGIYLTINLIIKRLI
                     KFTKVNIHAKREAPYLILNGFFFFILQLGTLATWSGDNFIISITLGVTYVAVFSITQR
                     LFQISTVPLTIYNIPLWAAYADAHARNDTQFIKKTLRTSLKIVGISSFLLAFILVVFG
                     SEVVNIWTEGKIQVPRTFIIAYALWSVIDAFSNTFASFLNGLNIVKQQMLAVVTLILI
                     AIPAKYIIVSH"
BASE COUNT          248 a          113 c          110 g          216 t
ORIGIN      
        1 atggctaact atgatgtatt ttgctggaat tgcgatcaat atcaatgtta caacagcaag
       61 catttgttgt ttaactatgt tcaaaccatt taaaaagctt gcaaatgtat tcgaaaaagc
      121 atcaataaca gaccataaag cataagctat tatgaatgtt cgaggtacct gaatctttcc
      181 ttctgtccaa atattaacga cttcactacc gaacactact aatatgaagg ccaataagaa
      241 tgatgaaata cccactattt tcaatgatgt tctgagcgtc ttttttataa attgagtatc
      301 attgcgtgca tgagcatctg cataagcagc ccataacggg atgttataaa tcgtaagagg
      361 gaccgtagat atttgaaata atctctgtgt aatgctaaaa acagcaacat aagtaacacc
      421 caatgttata gatattataa agttatcacc actccatgtt gccagagtgc ctaactgtaa
      481 aataaaaaag aaaaaaccgt ttaatatcaa atatggagct tctcttttag catgtatgtt
      541 aacttttgta aactttatta atcgctttat aataagatta attgttaaat agattcccga
      601 tatgtattga ataccaagag tgctgacaat taaaactggt agtcccgcat gtagtttcga
      661 cgatattact agagtaataa tagataa
//
LOCUS       DABSYT020000397          686 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.403, whole genome shotgun sequence.
ACCESSION   DABSYT020000397 DABSYT020000000
VERSION     DABSYT020000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.403"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..79)
                     /locus_tag="ID725_RS27165"
                     /old_locus_tag="pgaptmp_005433"
     CDS             complement(<1..79)
                     /locus_tag="ID725_RS27165"
                     /old_locus_tag="pgaptmp_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGV7853790.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="ID725_RS27170"
                     /old_locus_tag="pgaptmp_005434"
     CDS             complement(91..489)
                     /locus_tag="ID725_RS27170"
                     /old_locus_tag="pgaptmp_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGV7853791.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..>686)
                     /locus_tag="ID725_RS27175"
                     /old_locus_tag="pgaptmp_005435"
     CDS             complement(531..>686)
                     /locus_tag="ID725_RS27175"
                     /old_locus_tag="pgaptmp_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGV7853792.1"
                     /translation="ADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
BASE COUNT          140 a          218 c          178 g          150 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgc
//
LOCUS       DABSYT020000398          686 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.404, whole genome shotgun sequence.
ACCESSION   DABSYT020000398 DABSYT020000000
VERSION     DABSYT020000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.404"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>686
                     /locus_tag="ID725_RS27180"
                     /old_locus_tag="pgaptmp_005436"
     CDS             <1..>686
                     /locus_tag="ID725_RS27180"
                     /old_locus_tag="pgaptmp_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HGV7853793.1"
                     /translation="VVSPEDRISHLELDGFNYFSQPELDTPISDSFSYLYDNFQIVDS
                     DVHIKLLHLNRETKQITPHRIILKRYFIDSFAKTSITDREKNIYPVICDSPDHFTSDI
                     YRHPFRIVLGNKTLYPSEELVKFISTSKEYLSNMDVINNVIVPQKTTKKNKLSIVSLN
                     SNIKNDIVLSGVMTGTSKIFHLNNSGDLLLTTSKTHGGGVVVIFKDFINNWWKYNLTL
                     ITVPIDNKLS"
BASE COUNT          252 a          104 c          102 g          228 t
ORIGIN      
        1 tcgttgtatc acctgaagat agaatttctc atctggagtt agatgggttt aattattttt
       61 cacaacccga gttagatact ccaatatctg actcattttc atatttatat gataattttc
      121 agatcgttga tagtgatgtg catattaagc ttcttcatct caacagagaa acaaagcaaa
      181 taacaccaca tcgtattatt cttaaaagat actttataga ctcatttgct aaaacaagta
      241 tcactgacag agaaaagaat atatatcctg tgatttgtga ttcacctgat cacttcacaa
      301 gcgacatata taggcatcca tttagaatag ttctcgggaa taagacattg tatccatcag
      361 aagaacttgt aaaattcata agcacctcaa aagaatattt aagcaatatg gatgtgatta
      421 acaatgttat tgttcctcaa aaaacaacaa agaaaaacaa attatccata gttagtttaa
      481 atagcaatat caaaaatgat attgtattgt ccggagtaat gaccggaaca tcaaagattt
      541 ttcatctaaa taattcaggt gatttattgt taacaacctc aaaaactcat ggtggtgggg
      601 ttgtggttat ttttaaagat tttataaata actggtggaa atataatctt actttaataa
      661 cagttcctat tgataataaa ttgtca
//
LOCUS       DABSYT020000399          667 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.405, whole genome shotgun sequence.
ACCESSION   DABSYT020000399 DABSYT020000000
VERSION     DABSYT020000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.405"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..243
                     /locus_tag="ID725_RS27185"
                     /old_locus_tag="pgaptmp_005437"
     CDS             <1..243
                     /locus_tag="ID725_RS27185"
                     /old_locus_tag="pgaptmp_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGV7853794.1"
                     /translation="AEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRW
                     QLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            240..446
                     /locus_tag="ID725_RS27190"
                     /old_locus_tag="pgaptmp_005438"
     CDS             240..446
                     /locus_tag="ID725_RS27190"
                     /old_locus_tag="pgaptmp_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGV7853795.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            443..>667
                     /locus_tag="ID725_RS27195"
                     /old_locus_tag="pgaptmp_005439"
     CDS             443..>667
                     /locus_tag="ID725_RS27195"
                     /old_locus_tag="pgaptmp_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGV7853796.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQ"
BASE COUNT          169 a          170 c          210 g          118 t
ORIGIN      
        1 gctgaagagc aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa
       61 aagcgacgca atgaggcgct cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt
      121 atttcccgct ggcagctgga tctcagtgca ctgctggcga gcctgcagga agaggatggt
      181 gcagcaacca acaagaaaac actggcagaa tacgcccgtg ccttatccgg agaggatgaa
      241 tgacgcgaca ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggaa
      301 aacgggtggc aacggtacag aaagacggac ggcgagtgga gtttacgacc acttccgtgt
      361 ctgacctgaa aaaatacatt gctgagctgg aagtgcagac cggcatgaca cagcgacgca
      421 ggggaccagc aggattttat gtatgaaaat gtccaccatt cccacccttc tggggccgga
      481 cggcatgaca tcgctgcgtg aatatgccgg ttatcacggc ggtggcagcg gatttggtgg
      541 gcagttgcgg gcgtggaacc caccgggtga aagtgtggat gcagccctgc tgcccaactt
      601 tacccgtggc aatgcccgcg cagacgatct ggtacgcaat aacggctatg ccgccaacgc
      661 catccag
//
LOCUS       DABSYT020000400          664 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.406, whole genome shotgun sequence.
ACCESSION   DABSYT020000400 DABSYT020000000
VERSION     DABSYT020000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.406"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>664
                     /locus_tag="ID725_RS27200"
                     /old_locus_tag="pgaptmp_005440"
     CDS             <1..>664
                     /locus_tag="ID725_RS27200"
                     /old_locus_tag="pgaptmp_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGV7853797.1"
                     /translation="PFGSPVAITPYTLMQAITAEGDVVVSGATEPDWYYVIVLAGQSN
                     AMAYGEGLPLPDSYDAPDPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQDMSTL
                     NHPKADLSKGQYGCVGQGLHIAKKLLPYIPNNAGILLVPCCRGGSAFTQGAEGTFSES
                     TGASQDSARWGVGKPLYQDLISRTKAALQKNPKNVLLAVCWMQGEFDMSAATHAQQPA
                     LFT"
BASE COUNT          145 a          177 c          205 g          137 t
ORIGIN      
        1 agccgtttgg tagtccggtg gccataaccc cttataccct gatgcaggcg attacagcag
       61 aaggtgatgt ggtggtcagt ggtgcaactg agccggattg gtactacgtc atcgtactgg
      121 ccgggcagtc caatgccatg gcttacggtg aagggcttcc gctgccggat tcatacgatg
      181 ctccggatcc gcgcattaaa cagctggcgc gccgcagtac agtgacgccg ggtggggctg
      241 cctgcagata taacgatatt attccggccg accactgcct gcatgatgtg caggatatga
      301 gtacgctgaa tcatccgaag gcagacctga gcaaagggca gtacggctgt gtcggccagg
      361 ggttacatat tgccaaaaaa ctgctcccgt atatcccgaa taacgcgggg atcctgctgg
      421 taccatgctg tcgtggtggt tcggcattta cccagggcgc ggaggggaca ttcagcgagt
      481 ccacgggggc cagccaggat tcggcacgct ggggggtggg caagccgtta tatcaggatc
      541 tgatttcccg caccaaagcg gcattgcaga aaaatcccaa aaacgttctg ctggccgtct
      601 gctggatgca gggagagttt gacatgagcg ccgccaccca cgcacagcaa cctgcgctgt
      661 ttac
//
LOCUS       DABSYT020000401          664 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.407, whole genome shotgun sequence.
ACCESSION   DABSYT020000401 DABSYT020000000
VERSION     DABSYT020000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.407"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            1..651
                     /locus_tag="ID725_RS27205"
                     /old_locus_tag="pgaptmp_005441"
                     /pseudo
     CDS             1..651
                     /locus_tag="ID725_RS27205"
                     /old_locus_tag="pgaptmp_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
BASE COUNT          241 a          113 c          120 g          190 t
ORIGIN      
        1 cttgatttac agaaacaatt tggtgaaaac cgcatttcaa ttaaagatgt taactcttta
       61 acctctcttt caaaatcaga aaataatcac aattatcaaa ctgaaatgtt gcttcgatgg
      121 aactatcctg ccgcctcaga cctgctcagg atgtatatcc ttaaagagca tggtggtatt
      181 tatacagaca cagatatgat gcctgcatac tctaaacaag taatttttaa aattatgatg
      241 cagacaaacg gagataatcg tttcttggaa gatttgaaac tacgtcgtgc aatatctgat
      301 ggtgtattaa gatatgttaa taaccaaaat attgatgaag ttaactataa tgaaatcagt
      361 gatgcagata aaaacattat caagaagata ttaacagaaa tatctaaaat gccagaagat
      421 agtattttta ctaagatcaa tacaaggatt cctcgagaca caatgcccat ccttcgtcgt
      481 tatcacctat ggcctgatgg atggaatatt cgtgggctca atggattcat gctatcacat
      541 aaaggtagtg aagtgattga tgctgttatc gcaggccaga atcaggctta cagggaacta
      601 agaagaataa gagataatat tcatagtgaa atatacttca aacaaactga ttgaaccgcc
      661 ccgg
//
LOCUS       DABSYT020000402          644 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.408, whole genome shotgun sequence.
ACCESSION   DABSYT020000402 DABSYT020000000
VERSION     DABSYT020000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.408"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            143..>644
                     /locus_tag="ID725_RS27210"
                     /old_locus_tag="pgaptmp_005442"
     CDS             143..>644
                     /locus_tag="ID725_RS27210"
                     /old_locus_tag="pgaptmp_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7853798.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGN"
BASE COUNT          197 a          112 c          145 g          190 t
ORIGIN      
        1 attcatgaaa tctataaatt aaagatttgt cacttattgg atttagtatg cttgatttaa
       61 atggcggaca attattgtta ttattaagtc taatgtcatt taaaggcatt atcatattta
      121 cgaagagatc aggatgataa cgatgaaaaa aagtgtattg acggcgttta taactgtggt
      181 atgtgcaacg tccagcgtta tggctgctga tgataatgct atcacggatg gctcagtaac
      241 atttaatggt aaagttattg ctccagcttg taccctggta gctgcgacga aagattccgt
      301 ggtgactttg ccagatgtta gtgccacgaa gttgcaaacc aatggtcagg tttctggcgt
      361 gcaaactgat gtgccaattg aattaaaaga ttgtgatact accgtaacaa aaaatgcaac
      421 gttcaccttt aatggcactg cggatactac tcagattaca gcgtttgcta accaggcctc
      481 atctgatgct gctacaaacg tggccctgca aatgtatatg aatgatggta caacggccat
      541 caagccagac acagaaaccg ggaacatttt gttgcaagat ggagatcaga cgttgacttt
      601 taaagttgat tatatcgcta cggggaaagc gacttctggt aatg
//
LOCUS       DABSYT020000403          641 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.409, whole genome shotgun sequence.
ACCESSION   DABSYT020000403 DABSYT020000000
VERSION     DABSYT020000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.409"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            1..501
                     /locus_tag="ID725_RS27215"
                     /old_locus_tag="pgaptmp_005443"
     CDS             1..501
                     /locus_tag="ID725_RS27215"
                     /old_locus_tag="pgaptmp_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7853799.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIWINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNQLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
     gene            531..>641
                     /locus_tag="ID725_RS27220"
                     /old_locus_tag="pgaptmp_005444"
     CDS             531..>641
                     /locus_tag="ID725_RS27220"
                     /old_locus_tag="pgaptmp_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HGV7853800.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDA"
BASE COUNT          227 a          113 c          104 g          197 t
ORIGIN      
        1 atgcaagtct tttccagtga tgtctacttc actgtaggca ctaatgcatt attagcatcc
       61 caaaaggaat attacagcga tttagttgcg ttggttgatc ttggacattc gttcgttgtt
      121 attgatgagc atcaacatcg aaatctcaaa ccaaatacag aacctgtaaa catccttttg
      181 agtaacaatt tcatctggat caataagaat attacattat ctgatttaac gcatttcctg
      241 gtttcaaatc ttcatacaca aaatgtgtat tccacacaag aaccacttac ccatgatgag
      301 atagatatcc tgcggttatg tgtttcatat tcattaaagc aaattgccat tattaaagga
      361 attgattata aaacagtatc atatcacaaa ataagagccc tcaatcaact taacataaaa
      421 ggtactgttg agttgtttat agcactctgt gaatgggata aacattattt taaactccaa
      481 tcttgcgtaa gagaaagttg atatgtcatt agtaatgata taatagtaaa atcataataa
      541 ccatcgcgct gatatcgttg ctctctgcat gcaccagaga aaattcatta aaggagaatc
      601 aaaataaaag aagtggcgaa tcccaagcga tagtagatgc a
//
LOCUS       DABSYT020000404          640 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.410, whole genome shotgun sequence.
ACCESSION   DABSYT020000404 DABSYT020000000
VERSION     DABSYT020000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.410"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..388)
                     /locus_tag="ID725_RS27225"
                     /old_locus_tag="pgaptmp_005445"
     CDS             complement(<1..388)
                     /locus_tag="ID725_RS27225"
                     /old_locus_tag="pgaptmp_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853801.1"
                     /translation="MKAKYVILSVLNKQELFYLKSRYKEMKKLANKHEVSLVEQNFID
                     AIISMVGYCSLGFGLVSIVNGILLVFSFFQDNMLKSFFLSSIVSIAMFAILIIHRKLD
                     TKFSYQLCLKMFYLKIKLFCYCIPAPV"
BASE COUNT          247 a          113 c           85 g          195 t
ORIGIN      
        1 caactggtgc aggaatacaa taacagaata acttaatttt aagataaaac attttgaggc
       61 ataattggta agagaattta gtatccagtt ttctgtgtat gataagaata gcgaacatgg
      121 caatactaac gattgaagac agaaaaaaac ttttcaacat attatcctga aaaaatgaga
      181 atacaagcaa tatcccatta actatactta ccaacccaaa ccccaaagag caataaccca
      241 ccattgaaat aatagcatca atgaaatttt gttctacgag agagacttca tgcttattag
      301 caagtttctt catttctttg tatcttgatt ttaaatagaa gagttcttgt ttattaagta
      361 cagagagaat cacgtatttt gctttcatta ttccccctta aaaacacttt acaaaatcga
      421 tcaaagtcat gatgaatgcc attatgcaca taaacaacac aaagcgtgat atttaaatca
      481 ataaattaca ctctaaaatg aactataact acagtttatt taatagcata gttaattcct
      541 ttcctgaata tcaggaagta attatcgcac gtcctatttt cacggtaaat gaaagactat
      601 tctgcatact cttgaaaaga aagcataacc taagcccctt
//
LOCUS       DABSYT020000405          619 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.411, whole genome shotgun sequence.
ACCESSION   DABSYT020000405 DABSYT020000000
VERSION     DABSYT020000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.411"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..129
                     /locus_tag="ID725_RS27230"
                     /old_locus_tag="pgaptmp_005446"
     CDS             <1..129
                     /locus_tag="ID725_RS27230"
                     /old_locus_tag="pgaptmp_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853802.1"
                     /translation="IAVAEMFHNAAWGVRTLWFELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(229..444)
                     /locus_tag="ID725_RS27235"
                     /old_locus_tag="pgaptmp_005447"
                     /pseudo
     CDS             complement(229..444)
                     /locus_tag="ID725_RS27235"
                     /old_locus_tag="pgaptmp_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(488..>619)
                     /locus_tag="ID725_RS27240"
                     /old_locus_tag="pgaptmp_005448"
     CDS             complement(488..>619)
                     /locus_tag="ID725_RS27240"
                     /old_locus_tag="pgaptmp_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGV7853803.1"
                     /translation="NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          168 a          148 c          142 g          161 t
ORIGIN      
        1 atagccgttg cggaaatgtt tcataacgca gcatggggag tgcgaacatt gtggtttgaa
       61 ctcgtcacaa aaatagatat agatatacat aaaaagaaca gatatgcgag ttatgatctt
      121 gatcgatagg aatttaaatc ccaaaaagat taaaaaaaca cccaaaaacg gatgtttatt
      181 caacactgcc tgcgtgccat gagtgcaccg ccgtcaatta aatggatcta tttcatcctg
      241 accagcatag tgctccagac ggctcatcgg caccggcggt tttttcaggt aagagccgaa
      301 gtacaccgcc acatgtgtgg cgttatccat cacccgggat acgttgacat tccagccccg
      361 ccggtaatga ctgtccagga agcgattcca gtcccgttta ctgcttcctt ctgctgccag
      421 cgcatccggt atcaccaggt ccggtgaggt gtactggcaa tagcggacac taccatttgt
      481 tcttttttta agcagccatc tgatgatatt tttccctgaa ggctgccggg gagatattcc
      541 ccagacgaga gtgacgacgc tgacgattgt agaaaatctc aatgtattcc cgtattactg
      601 agatggcttc atcccggtt
//
LOCUS       DABSYT020000406          617 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.412, whole genome shotgun sequence.
ACCESSION   DABSYT020000406 DABSYT020000000
VERSION     DABSYT020000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.412"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..127)
                     /locus_tag="ID725_RS27245"
                     /old_locus_tag="pgaptmp_005449"
     CDS             complement(<1..127)
                     /locus_tag="ID725_RS27245"
                     /old_locus_tag="pgaptmp_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGV7853804.1"
                     /translation="MITSERRIIMIPKRLKEAREAANFSQEKLAQLVDIESVNSRS"
     gene            299..586
                     /locus_tag="ID725_RS27250"
                     /old_locus_tag="pgaptmp_005450"
     CDS             299..586
                     /locus_tag="ID725_RS27250"
                     /old_locus_tag="pgaptmp_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGV7853805.1"
                     /translation="MAYYENMRYDLLNKIFPDLTPVQAQCVLMYSFGMSSLEISGCVG
                     VSRQMIDKNLHAAAKKMNVNNLIALKPAVVIGILLEVLASLPVKDDLTNGD"
BASE COUNT          170 a           85 c          129 g          233 t
ORIGIN      
        1 ttgaacgact gtttactgat tcaatatcaa ctagttgagc aagtttttcc tgtgaaaaat
       61 ttgcagcttc tcgtgcttct tttagtcttt ttgggatcat gattattctc cgttcagatg
      121 tgatcataat aacggattgc aaaaagattg tgttttgatg tgcaaaacat attgacaatt
      181 gttttgcctt tagtatagtt gctgtaatca aattatcacg tagctaaatc atgtaatggt
      241 ttgattttaa atgaaatgtt tgtagtggag ttgttattgg tgtcttaagg gtgtggttat
      301 ggcctattac gagaatatgc gttatgactt gcttaataaa atttttcctg acttaacccc
      361 agtgcaagca cagtgtgtat taatgtattc ctttgggatg agttcacttg aaatatcagg
      421 gtgcgttggt gtttctcgcc aaatgattga taagaactta catgcggcag cgaaaaaaat
      481 gaatgttaat aatcttattg cacttaaacc tgccgttgta attggtattt tgcttgaggt
      541 attagcatct ttgcctgtga aagatgattt gactaatgga gattaattta gttgtttata
      601 aaagcccttt tcagggc
//
LOCUS       DABSYT020000407          609 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.413, whole genome shotgun sequence.
ACCESSION   DABSYT020000407 DABSYT020000000
VERSION     DABSYT020000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.413"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>609
                     /locus_tag="ID725_RS27255"
                     /old_locus_tag="pgaptmp_005451"
     CDS             <1..>609
                     /locus_tag="ID725_RS27255"
                     /old_locus_tag="pgaptmp_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HGV7853806.1"
                     /translation="VIDKQESKIVYRDQKTESPVFTDKGDFVVAGKNPSKEEIGIMLE
                     YSKEKFGGVLKLTGSEEFKKECALVAAERDMNIILRPEKYQQMLLEHKAELESKAMQQ
                     TEQESQQQTQVDNEISKADVHQNQAPAEEAKQEIYLVSHTNVHEQNEGVIFYSKEAAY
                     SYFEEDKKMAIEKYEANPHLGNGFEGALLVSKTIAASELSSYP"
BASE COUNT          209 a          116 c          149 g          135 t
ORIGIN      
        1 tgtaatagat aaacaagaaa gcaagattgt ttatcgtgac caaaaaacag agtcccccgt
       61 atttactgat aagggtgatt ttgttgttgc cggaaagaat ccttctaaag aagagatcgg
      121 catcatgctg gagtattcga aagagaagtt tggcggtgtg ctcaaactta ccggaagcga
      181 agagtttaag aaagaatgtg ctcttgtagc ggcagaacgg gatatgaata ttattctgcg
      241 ccctgagaaa taccaacaga tgttgctgga gcataaagcg gagcttgagt ctaaagcaat
      301 gcagcaaact gaacaggaat cccagcaaca aacccaggtt gataatgaaa tcagcaaggc
      361 agatgttcat cagaaccagg cgccagcgga agaggctaaa caagaaattt atctggtcag
      421 ccatacaaac gtacacgaac agaacgaagg ggtcattttc tattcaaaag aggctgctta
      481 cagctacttt gaagaagaca agaaaatggc aatcgagaaa tacgaagcga acccacattt
      541 gggaaatgga tttgaaggtg cgttgcttgt ttccaagacc atagcagcca gcgagctgtc
      601 ctcttatcc
//
LOCUS       DABSYT020000408          607 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.414, whole genome shotgun sequence.
ACCESSION   DABSYT020000408 DABSYT020000000
VERSION     DABSYT020000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.414"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..128)
                     /locus_tag="ID725_RS27260"
                     /old_locus_tag="pgaptmp_005452"
     CDS             complement(<1..128)
                     /locus_tag="ID725_RS27260"
                     /old_locus_tag="pgaptmp_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin subunit A"
                     /protein_id="HGV7853807.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIR"
     gene            complement(219..295)
                     /locus_tag="ID725_RS27265"
                     /old_locus_tag="pgaptmp_005453"
     tRNA            complement(219..295)
                     /locus_tag="ID725_RS27265"
                     /old_locus_tag="pgaptmp_005453"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(259..261),aa:Arg,seq:tct)
     gene            complement(309..385)
                     /locus_tag="ID725_RS27270"
                     /old_locus_tag="pgaptmp_005454"
     tRNA            complement(309..385)
                     /locus_tag="ID725_RS27270"
                     /old_locus_tag="pgaptmp_005454"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(349..351),aa:Arg,seq:tcg)
     gene            complement(393..468)
                     /locus_tag="ID725_RS27275"
                     /old_locus_tag="pgaptmp_005455"
     tRNA            complement(393..468)
                     /locus_tag="ID725_RS27275"
                     /old_locus_tag="pgaptmp_005455"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(433..435),aa:Ile,seq:cat)
     gene            complement(509..>607)
                     /locus_tag="ID725_RS27280"
                     /old_locus_tag="pgaptmp_005456"
     CDS             complement(509..>607)
                     /locus_tag="ID725_RS27280"
                     /old_locus_tag="pgaptmp_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGV7853808.1"
                     /translation="MALGRRAIGVELESGRFEQTVREVQNVVSQNG"
BASE COUNT          171 a          161 c          141 g          134 t
ORIGIN      
        1 cgtatactat ttaacgaaga gacataactt tgttgggtcg aaaagtctat cgtaaactcc
       61 cgggaatagg ataccgaaga aaaacccagt aacaggcaca gtacccattt aaataatata
      121 cacttcatat acaggtgttc cttttggctg aagtaatcag caccagaccc ggcgcagata
      181 taaaaaaggc ccgcaaaagc gagcctggta aataaatatg gcgcgttgta ctggattcga
      241 accagtgacc gattgctcag aaggcaattg ctctgtccgg ctgagctaac aacgcataat
      301 gcagataatg gaccgccatc gaggactcga accccgcgca gccagcttcg aaggctggcg
      361 ctctatcccg atgagctaat ggcggtatgt gatggtggcc cttgctggat ttgaaccagc
      421 gacctggcga ttatgagtcg ctcgctctca ccactgagct aaagggccgg gcgcaggata
      481 ataacggtac gtaactaatt ctgcaatatc atccgttctg actgactaca ttctgaactt
      541 ccctgaccgt ctgctcaaaa cgcccgctct ccagctcaac gccaattgca cgacgcccta
      601 gcgccat
//
LOCUS       DABSYT020000409          605 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.415, whole genome shotgun sequence.
ACCESSION   DABSYT020000409 DABSYT020000000
VERSION     DABSYT020000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.415"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>605
                     /locus_tag="ID725_RS27285"
                     /old_locus_tag="pgaptmp_005457"
     CDS             <1..>605
                     /locus_tag="ID725_RS27285"
                     /old_locus_tag="pgaptmp_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGV7853809.1"
                     /translation="RLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESDRLT
                     HRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPETGE
                     LLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDRLHR
                     ETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWND"
BASE COUNT          145 a          173 c          201 g           86 t
ORIGIN      
        1 cggctggtac agcagcgcgg ctttgacggg cggacgcaac gttaccacta tgacctgacc
       61 ggaaaactca cgcagagtga agatgagggg cttgtcaccc tctggcacta cgacgaatcg
      121 gaccgcctca ctcaccgcac ggtgaacggc gaaccggcag agcagtggca gtacgacgag
      181 cacggctggc tgacagaaat cagccacctg agcgaaggcc atcaggtggc ggtgcattac
      241 ggttatgatg ataagggccg cctggccggg gagcgccaga cggtgcataa cccggagacg
      301 ggggaactgc tgtggcagca tgagacagag cacgcataca acgaacaggg tctggcaaac
      361 cgcgtcacgc cggacagcct gccgcgggtg gagtggctga cctacggcag cggttatctt
      421 gcggggatga agctgggcgg gacgccgctg gtggagttca cgcgcgacag gctgcaccgc
      481 gagacggtgc gcagcttcgg caataacgca tacgaactga ccagcacata cactcccgca
      541 ggccatttac agagccagcg cctgaacagc caggtgtatg accgtgacta cgactggaat
      601 gacaa
//
LOCUS       DABSYT020000410          603 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.416, whole genome shotgun sequence.
ACCESSION   DABSYT020000410 DABSYT020000000
VERSION     DABSYT020000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.416"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..>603)
                     /locus_tag="ID725_RS27290"
                     /old_locus_tag="pgaptmp_005458"
     CDS             complement(<1..>603)
                     /locus_tag="ID725_RS27290"
                     /old_locus_tag="pgaptmp_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGV7853810.1"
                     /translation="ESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNEKGEAVT
                     WQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRV
                     YARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDGALFPGR
                     IMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCS"
BASE COUNT          117 a          222 c          139 g          125 t
ORIGIN      
        1 ttactgcatc tgtccttccg gatatccgtg gtggggttgt cgaactcatc cgccactgcc
       61 ggaccgttat acccgcattc atcgccccgg taatcccaca tacaggtgtt cgccagcatg
      121 atgcgaccgg gaaacagcgc cccgtccgtc tccgtcggtg tcgccagcac aaacgaggcc
      181 gtcatggccg tcagcgatga catctgctcc accacccacc ggtccgtcag ctcctgctca
      241 gggtctgcct caggattgcc tgccacaaaa ttcaccgcat ccagaaaacg ggcatacacc
      301 cggcggcgga ccaccgtggc ccccaccagg ctctgcaggt cctccgccat cccggtgacc
      361 agaccaaaca gattcgacac cgtcagcgac ggtctggcac tgctgccctt cccgttcatc
      421 tcaaagccac tgccgtcaat cgggtatgcc tgatattgcc gcccctgcca ggtaaccgcc
      481 tccccttttt cattcagctc attgcagaaa aaataccgct caccgccctg cgccgtcagg
      541 tcgatttccc agagtaccac ccgcggtgac tgctctgatt taaccgactc gttcagactt
      601 tct
//
LOCUS       DABSYT020000411          602 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.417, whole genome shotgun sequence.
ACCESSION   DABSYT020000411 DABSYT020000000
VERSION     DABSYT020000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.417"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..145)
                     /locus_tag="ID725_RS27295"
                     /old_locus_tag="pgaptmp_005459"
     CDS             complement(<1..145)
                     /locus_tag="ID725_RS27295"
                     /old_locus_tag="pgaptmp_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGV7853811.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIK"
     gene            207..602
                     /locus_tag="ID725_RS27300"
                     /old_locus_tag="pgaptmp_005460"
                     /pseudo
     CDS             207..602
                     /locus_tag="ID725_RS27300"
                     /old_locus_tag="pgaptmp_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          168 a          148 c          138 g          148 t
ORIGIN      
        1 gttttatgcc gccagtatcc cttaacatag tgaagatcgt tccgggtttt gaacccagga
       61 tattcgctat ttcactgaag cctgttccgt tcttccatag ttcaaaaaca gaggcttttt
      121 cctctgctgt aaatgttcgt ctcattcaaa aaacctccgc aaccccatgt tttcacataa
      181 ctgttgcgtt gaccaattga atctacaaca gcgtattttg cacaggagtc gctgaaaaat
      241 acgcgttaat cgaacaatgg cgacaacaat ttcccattga agcgatgtgt caggtatttg
      301 gtgtatccag gagcggttat tacaactggg tacagcatga accctcagac agaaaacaaa
      361 gtgatgagcg gctaaaactg gagattaagg tggcacatat ccgcactcgc gaaacatatg
      421 gaacccggcg gctccagacg gagctggcag agaatggcat catcgttggt cgtgaccgac
      481 tggcacgtct tcgtaaggag ctaaggctac gctgtaagca gaaacgcaag ttcagggcga
      541 ctacgaactc gaaccacaat ctgccagttg cgccaaatct gctgaaccag acgttcgctc
      601 ct
//
LOCUS       DABSYT020000412          597 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.418, whole genome shotgun sequence.
ACCESSION   DABSYT020000412 DABSYT020000000
VERSION     DABSYT020000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.418"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>597
                     /locus_tag="ID725_RS27305"
                     /old_locus_tag="pgaptmp_005461"
     CDS             <1..>597
                     /locus_tag="ID725_RS27305"
                     /old_locus_tag="pgaptmp_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853812.1"
                     /translation="RPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQMVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDSPSDVVWPYT"
BASE COUNT          131 a          163 c          191 g          112 t
ORIGIN      
        1 cccgtccttt taacatccgg atggtccggg agacggcgga cagcacctcg gaccagctgc
       61 agaataagac gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga
      121 acacggcgat tgtggggctg caggtggatg cggagcagtt tggtggccag cagatgacgg
      181 tgaactacca tatccgaggt cgcatcatcc aggtgccgtc aaactatgac ccggaaaaac
      241 gcacgtacag cggcatctgg gacggcagcc tgaaaccggc atacagcaac aacccggcct
      301 ggtgcctgtg ggacatgctg actcacccgc gctacggcat gggaaaacgc ctgggggcgg
      361 cggatgtgga caagtgggcg ctgtatgcca ttgcgcagta ctgcgaccag atggtccctg
      421 atggtttcgg gggcacagag ccgcggatga cctttaatgc gtacctgtca caacagcgta
      481 aggcgtggga cgttctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg
      541 gccagacgct gacgttcgtt caggacagcc cgtcggatgt ggtgtggccg tacacca
//
LOCUS       DABSYT020000413          593 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.419, whole genome shotgun sequence.
ACCESSION   DABSYT020000413 DABSYT020000000
VERSION     DABSYT020000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.419"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..330)
                     /locus_tag="ID725_RS27310"
                     /old_locus_tag="pgaptmp_005462"
     CDS             complement(<1..330)
                     /locus_tag="ID725_RS27310"
                     /old_locus_tag="pgaptmp_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGV7853813.1"
                     /translation="MNDNFFTFRKIKVTGFNKLDAIIEFGSKLTILYGGSDSGKTYIY
                     YLIRYLLGSEKLKNKDIDHAQGYDLAYLEFNFQGRVMTIERSLQDSAHYRLYDSSIEN
                     VSEANLLM"
     gene            complement(323..>593)
                     /locus_tag="ID725_RS27315"
                     /old_locus_tag="pgaptmp_005463"
     CDS             complement(323..>593)
                     /locus_tag="ID725_RS27315"
                     /old_locus_tag="pgaptmp_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HGV7853814.1"
                     /translation="ALKREIVLESLALLQGYELASKIYTHRGFVYKASEKTYAFTNSL
                     HNEYVAQMEHNINLVVKLYSDIPDEQLQSIIKNKIGKYDMEFNYE"
BASE COUNT          203 a          109 c           68 g          213 t
ORIGIN      
        1 catcaacaga ttagcttcac taacattttc aatactcgaa tcatataatc tgtaatgggc
       61 gctatcctga agagacctct caattgtcat taccctacct tgaaaattaa attccagata
      121 ggctaaatca taaccttgag catgatcgat atctttattt tttagttttt cactccctaa
      181 taaatatcga atcaaataat atatgtatgt ttttccagag tcagaaccac catataaaat
      241 agtcaattta gaaccaaatt caattatagc atctaattta ttgaatccgg ttacctttat
      301 ttttctgaac gtaaaaaaat tgtcattcat aattaaattc catatcatat ttgccaattt
      361 tattttttat aattgattgc aactgctcat caggaatatc actatataac ttaaccacca
      421 aatttatatt atgctccatc tgcgcaacat attcattatg tagagaattt gtaaatgcat
      481 atgttttttc agaagcttta tatacaaaac cacgatgtgt ataaattttt gaggctagtt
      541 catagccttg caacaaagcc aatgattcta aaacaatctc tcgttttagg gct
//
LOCUS       DABSYT020000414          592 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.420, whole genome shotgun sequence.
ACCESSION   DABSYT020000414 DABSYT020000000
VERSION     DABSYT020000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.420"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            52..>592
                     /locus_tag="ID725_RS27320"
                     /old_locus_tag="pgaptmp_005464"
     CDS             52..>592
                     /locus_tag="ID725_RS27320"
                     /old_locus_tag="pgaptmp_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGV7853815.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGAD"
BASE COUNT          197 a          114 c          147 g          134 t
ORIGIN      
        1 ggagtatcaa cagaaactta aaaaactgcg aaacagcaga agtgaggttg catgaatatc
       61 tacgaaagaa ttgatggcag caaataccga aatatttggg tagttggcga tctgcacgga
      121 tgctacacga acctgatgaa aaaactggag acgataggat tcgacaccaa aaaagacctg
      181 cttatctcgg tgggcgattt ggttgatcgc ggtacagaga acgtagaatg cctggaatta
      241 atcacattcc cctggttcag agctgtacgt ggaaaccatg agcaaatgat gattgatggc
      301 ttatcagagc gtggaaacgt caatcactgg ctgcttaatg gcggtggctg gttctttaat
      361 ctcgattacg acaaagaaat tctggctaaa gctcttgccc ataaagcaga tgaacttccg
      421 ttaatcatcg aactggtgag taaaggaaaa aaatatgtca tctgccacgc cgattatcct
      481 tgtgataaat acgagtttgg aaagccagtt gatcatcagc aggtaatctg gaaccgcgaa
      541 cgaatcagca actcacaaga cgggatcgtg aaagaaatca aaggcgcgga ca
//
LOCUS       DABSYT020000415          588 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.421, whole genome shotgun sequence.
ACCESSION   DABSYT020000415 DABSYT020000000
VERSION     DABSYT020000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.421"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..331)
                     /locus_tag="ID725_RS27325"
                     /old_locus_tag="pgaptmp_005465"
     CDS             complement(<1..331)
                     /locus_tag="ID725_RS27325"
                     /old_locus_tag="pgaptmp_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853816.1"
                     /translation="MAKNDFKAFATDRNANVMSQEEWEALPALISGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTAL"
     gene            complement(331..>588)
                     /locus_tag="ID725_RS27330"
                     /old_locus_tag="pgaptmp_005466"
     CDS             complement(331..>588)
                     /locus_tag="ID725_RS27330"
                     /old_locus_tag="pgaptmp_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
                     /protein_id="HGV7853817.1"
                     /translation="IANIDNQDMTISVWVFPDTDISDVSRELIAAIKQGYLTVKAAGV
                     WAGGIETPSVETPSEGSKFFGFDMDNEFISGFDVGAWGVLL"
BASE COUNT          160 a          167 c          110 g          151 t
ORIGIN      
        1 gaagagccgt tttcaccgtg ccgtccgatt tgatatcgcc aaacggattc ttgcggctca
       61 ggtattcaac agcaaacccc gatcccagca attcaacaaa accgggcaga tcaccattat
      121 caagcacatc ccgttgcgtt ttatcactta caaactgggc cagagctgca gcaataaagc
      181 tggcctgccg aataacctta ttgacttgcg cactggatgc tttccctgct gtaaatccgg
      241 atataagcgc aggcaacgct tcccattcct cctgcgacat aacattggca tttcgatcag
      301 ttgcaaacgc tttaaagtca tttttcgcca tcagagtaat actccccatg cccctacatc
      361 aaaaccactg atgaattcgt tatccatatc aaaaccaaaa aatttagagc cttccgatgg
      421 agtttccacc gaaggtgttt caatgccccc cgcccatacc ccggcggctt ttactgttag
      481 atacccctgt ttaattgccg caattaactc acgcgataca tctgaaatat cagtatcagg
      541 aaagacccag accgatatcg tcatgtcctg gttatcgatg ttggcgat
//
LOCUS       DABSYT020000416          576 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.422, whole genome shotgun sequence.
ACCESSION   DABSYT020000416 DABSYT020000000
VERSION     DABSYT020000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.422"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            27..305
                     /locus_tag="ID725_RS27335"
                     /old_locus_tag="pgaptmp_005467"
     CDS             27..305
                     /locus_tag="ID725_RS27335"
                     /old_locus_tag="pgaptmp_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853818.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            438..>576
                     /locus_tag="ID725_RS27340"
                     /old_locus_tag="pgaptmp_005468"
     CDS             438..>576
                     /locus_tag="ID725_RS27340"
                     /old_locus_tag="pgaptmp_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853819.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RD"
BASE COUNT          167 a          101 c          166 g          142 t
ORIGIN      
        1 ggccttttta tttgggggag agggaagtga acgatagcta ccgacagttt gaaaactggt
       61 ggtcaaaaga caaaagccag ttcacgggag acgatgaatt aaaagagttt gcctgggtga
      121 tatggcaggc atcgcgctct gctattgaac tggatatcga ctggcccgaa tcgaatgacg
      181 acctttggaa agatggtgaa gaaggtgctt atgcgatggg ttatgaggat gggcgtgaca
      241 aaacggtaat tgcagtaatg aaagccatca gggccgcagg aatcaaagaa aagaatttcg
      301 attaagcaaa tatcacttca ataaatcgct tttaaggcat cacaatcgct ctgtggtgag
      361 gtaagcacgt gcaaggtata ccgataagca gcgagaatga aaaatgcgtc agaatgcgtt
      421 tgaggaggtt ttaagaaatg agtacgatag ctgagcttgt cagggctaat tttcgtgaag
      481 agttggtgcg ttggtatcgg tatcgttcat cgtccagttt gccgcttgat gagttgtatg
      541 agcattcacc tgccgcacga cgctatccgc gtgacc
//
LOCUS       DABSYT020000417          573 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.423, whole genome shotgun sequence.
ACCESSION   DABSYT020000417 DABSYT020000000
VERSION     DABSYT020000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.423"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..336
                     /locus_tag="ID725_RS27345"
                     /old_locus_tag="pgaptmp_005469"
     CDS             <1..336
                     /locus_tag="ID725_RS27345"
                     /old_locus_tag="pgaptmp_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HGV7853820.1"
                     /translation="VKLVKGTKVKIIVWINEFQGEPILSGKHITETNFFEKNKDVIAG
                     AILIKDRKSDAFDTDIKKLTANSMTLTEALESKEFGLMAKSRLKRVFNDVYVQLDAIY
                     DPESVEANA"
     gene            333..>573
                     /locus_tag="ID725_RS27350"
                     /old_locus_tag="pgaptmp_005470"
     CDS             333..>573
                     /locus_tag="ID725_RS27350"
                     /old_locus_tag="pgaptmp_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853821.1"
                     /translation="MSDIELQQPERAVHEAEDQINDFVVDVFKKTGVRISKDDPVLSL
                     LFLHEKIQKRQSDLLKDDFTTLADAFKNVLSSLEEE"
BASE COUNT          212 a           89 c          114 g          158 t
ORIGIN      
        1 gttaaacttg taaaaggcac gaaagttaaa ataatcgtat ggattaatga gttccagggc
       61 gaaccgatac tttcagggaa gcatataact gaaacaaact tttttgaaaa aaataaagac
      121 gttattgctg gcgcaatatt gatcaaagat cgcaaaagcg atgcttttga tactgacata
      181 aaaaaattaa cagccaattc aatgacactg actgaggcgc ttgaatcgaa agagtttgga
      241 ttaatggcta aatcccgcct caagcgcgtt tttaacgatg tatatgtaca gcttgatgca
      301 atctatgacc ctgaaagtgt agaggccaat gcatgagtga tattgaatta caacagcccg
      361 aaagggctgt tcatgaagca gaagatcaga taaatgattt tgttgttgat gttttcaaga
      421 aaacaggggt aagaatatca aaagatgatc ctgtactatc cctgctattt ctccatgaaa
      481 aaattcaaaa gagacaatct gatttattaa aagatgattt caccacttta gctgatgcat
      541 tcaagaatgt attaagttca ttagaagaag aaa
//
LOCUS       DABSYT020000418          571 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.424, whole genome shotgun sequence.
ACCESSION   DABSYT020000418 DABSYT020000000
VERSION     DABSYT020000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.424"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            179..>571
                     /locus_tag="ID725_RS27355"
                     /old_locus_tag="pgaptmp_005471"
     CDS             179..>571
                     /locus_tag="ID725_RS27355"
                     /old_locus_tag="pgaptmp_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HGV7853822.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMS
                     HHLGYDKNQPKPGANSRNGYSTKTVITGDGHLELRTPRDRDGSFEPQLVKKNQTRITG
                     MDNQILSLYAKGLTTREIAAAFKELYDAD"
BASE COUNT          175 a          142 c          124 g          130 t
ORIGIN      
        1 cagaccaaca gaaacagcat tgtaaataaa atgtaataat tacatactat tagtgattct
       61 catgcatcgt aagcggctcg ccagaaccgt attgatattt actgaggagc ctgtacatag
      121 atttgtgtaa ttgcctgatt ttgatatgtt caatccaaca tcaaaagcag gttaatttat
      181 ggacgaaaaa cagttgcagg ctctggctaa cgaactggcc aaaaatctca aaacccctga
      241 agatctcagc cagttcgatc gcctgctgaa gaaaatcagc gttgaggcag ctctcaacgc
      301 tgaaatgtcc caccatctgg gctacgataa aaaccagcct aaaccgggtg ccaactcccg
      361 caatggctat tccacaaaga ccgttatcac cggtgatggc cacctggaac tacgcactcc
      421 gcgcgatcgt gatggctctt ttgaaccgca acttgtgaag aaaaaccaga cccggatcac
      481 cgggatggat aaccagatcc tgtcgttgta cgccaaaggc ctgacaaccc gcgaaatagc
      541 ggctgcgttt aaagagctgt atgacgctga t
//
LOCUS       DABSYT020000419          547 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.425, whole genome shotgun sequence.
ACCESSION   DABSYT020000419 DABSYT020000000
VERSION     DABSYT020000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.425"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            255..470
                     /locus_tag="ID725_RS27360"
                     /old_locus_tag="pgaptmp_005472"
     CDS             255..470
                     /locus_tag="ID725_RS27360"
                     /old_locus_tag="pgaptmp_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGV7853823.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            475..>547
                     /locus_tag="ID725_RS27365"
                     /old_locus_tag="pgaptmp_005473"
     CDS             475..>547
                     /locus_tag="ID725_RS27365"
                     /old_locus_tag="pgaptmp_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HGV7853824.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVA"
BASE COUNT          135 a          106 c          160 g          146 t
ORIGIN      
        1 ctggctaaga aggatagttg ggtttcacat gatacttata tctggcagta cattttctga
       61 cagacagtga tgggtgttgt caagatattg tgtcatttat aacctgaatc agggggggga
      121 gccggaatgt tatctggcat ttttagcaga gcctgaatgc cataatcacg gctcccggcg
      181 ttggccgtca gtgggcgaca ctggcggctt tttgttttcc tttactttca ttttctgtcg
      241 gcggtgacgg agacatacat cagatggaaa aaatcacaac aggtgtgtca tacaccacgt
      301 cagcggtggg gacgggatac tggttactgc agctgctgga caaagtctct ccgtcccagt
      361 gggtggcaat cggtgtgctg gggagtctgc tgtttggcct gctgacgtat ctgactaacc
      421 tgtatttcaa aatcagagag gaccgtcgta aggcggcgcg gggggagtaa agcgatgaag
      481 aaaaaatacg aactgggtgt taaagggata aataattacc cggataagat tactgttact
      541 gtggcac
//
LOCUS       DABSYT020000420          544 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.426, whole genome shotgun sequence.
ACCESSION   DABSYT020000420 DABSYT020000000
VERSION     DABSYT020000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.426"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            109..>544
                     /gene="nleB2"
                     /locus_tag="ID725_RS27370"
                     /old_locus_tag="pgaptmp_005474"
     CDS             109..>544
                     /gene="nleB2"
                     /locus_tag="ID725_RS27370"
                     /old_locus_tag="pgaptmp_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HGV7853825.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTED"
BASE COUNT          195 a           73 c          116 g          160 t
ORIGIN      
        1 gaaagcaaag atggcaaaac tgctggaggt tttgtggttg agtatgccaa tataattaat
       61 agattaaaga gttagttgtg aagaaaatat ggataaacag gacgacgaat gctttcaccg
      121 ataaggacaa ctttccataa ctcagtaaat atagtgcaga gttcaccctg tcaaacggtt
      181 tcttttgcag gaaaggaata tgagttaaag gtcattgatg aaaaaacgcc tattcttttt
      241 cagtggtttg aacctaatcc tgaacgatat aagaaagatg aggttccaat agttaatact
      301 aagcagcatc cctatttaga taatgtcaca aatgcggcaa ggatagagag tgatcgtatg
      361 ataggtattt ttgttgatgg cgatttttca gtcaaccaaa agactgcttt ttcaaaattg
      421 gaacgagatt ttgaaaatgt aatgataatc tatcgggaag atgttgactt cagtatgtat
      481 gacagaaaac tatcagatat ttatcatgat attatatgtg aacaaaggtt acgaactgaa
      541 gaca
//
LOCUS       DABSYT020000421          539 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.427, whole genome shotgun sequence.
ACCESSION   DABSYT020000421 DABSYT020000000
VERSION     DABSYT020000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.427"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            298..>539
                     /locus_tag="ID725_RS27375"
                     /old_locus_tag="pgaptmp_005475"
     CDS             298..>539
                     /locus_tag="ID725_RS27375"
                     /old_locus_tag="pgaptmp_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853826.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQA"
BASE COUNT          145 a           99 c          150 g          145 t
ORIGIN      
        1 ttaattattt gacgtggttt gatggcgtag atgcacgttg tgacatgtag atgataatta
       61 ttatcatttt gcgggtcctt tccggcgatc cgacaggtta cggggcggcg acctcgcggg
      121 ttttcgctat ttatgaaaat tttccgggat ccatgtccgg tttctcttca agttaactat
      181 atgaaaaata taaaaacagg tcttctgtga accggacatg aacaaaaaac agacatgtaa
      241 accggacatg accggttttg ttgtgattgt gaggtgagag tttttgcgag gtgaggagtg
      301 gctacgcaga ctgaagttgc caggcattta agtctgaccg atcgccagct tcgcagattg
      361 cagaaattgc cgggtgcccc gatatcgaat aagcgagggc aactggatct ggatgcctgg
      421 cgcgattttt acatatcgta tctgaggaga agtaaaaacg atgtgcctga tggcgatagc
      481 gaagacgact atgaggagaa attgcttatt gccagatggg aactgacagc agaacaggc
//
LOCUS       DABSYT020000422          534 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.428, whole genome shotgun sequence.
ACCESSION   DABSYT020000422 DABSYT020000000
VERSION     DABSYT020000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.428"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..186
                     /locus_tag="ID725_RS27380"
                     /old_locus_tag="pgaptmp_005476"
     CDS             <1..186
                     /locus_tag="ID725_RS27380"
                     /old_locus_tag="pgaptmp_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HGV7853827.1"
                     /translation="LGTYKKSTLKTEKITRGLFSNDEITLIYFSEYSKRIVQEVFVFN
                     VEDKKVKLKGYRYDSIN"
     gene            complement(208..327)
                     /locus_tag="ID725_RS27385"
                     /old_locus_tag="pgaptmp_005477"
                     /pseudo
     CDS             complement(208..327)
                     /locus_tag="ID725_RS27385"
                     /old_locus_tag="pgaptmp_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          178 a           86 c          128 g          142 t
ORIGIN      
        1 cttgggactt ataaaaaatc aacccttaaa actgaaaaaa tcactcgcgg attattttca
       61 aacgatgaaa ttacattgat atatttctca gaatattcaa aaagaattgt ccaagaggtt
      121 tttgtgttta atgttgaaga caaaaaagta aaattaaaag gttataggta tgattcgatt
      181 aattaaattt tttgatgatg acttcaatta gtagaacatg tatttttcga taaaagcacc
      241 gatgactttt tcatgaagtt ctacagagcg cgagaaacag atggttctgc gagtcagacg
      301 tttgattcag gttctcaggg gcagattcag ggaaagatca cagggaaaga tcatcggtgt
      361 gccgtaggca caaagatgca aaacgggacg ttttgcatgt aataagcata agcttgtgaa
      421 gagatggtgg caggcccacc gggatctgct gtcgaaagtc ggtcccaaac aaccccggct
      481 gctgtggtaa agagccatgg tagtgagcgc agggggaacg atcagacgag atct
//
LOCUS       DABSYT020000423          527 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.429, whole genome shotgun sequence.
ACCESSION   DABSYT020000423 DABSYT020000000
VERSION     DABSYT020000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.429"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..>527)
                     /locus_tag="ID725_RS27390"
                     /old_locus_tag="pgaptmp_005478"
     CDS             complement(<1..>527)
                     /locus_tag="ID725_RS27390"
                     /old_locus_tag="pgaptmp_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGV7853828.1"
                     /translation="KATSALKNTRSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKS
                     AATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGA
                     FGGGASASSGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVG
                     NLYRLMRGYAEGGYVG"
BASE COUNT          110 a          193 c          120 g          104 t
ORIGIN      
        1 cccacataac caccttccgc atagccgcgc atcagacggt aaagattccc cacacctatc
       61 cggctggttg cctctttcgt gaaaacaaac tccccgcggt gaactatccc cgcaggctca
      121 tatttgccgc ccgtccccgt aaatcctccg gtcgcgaaat ggaagttcgc cgccgcagcc
      181 tgaatggccg tccccgagga agcagatgca ccaccaccga aagcaccgcc aatggcgctg
      241 ccgatacgcc cgacaatgcc caccatggcc tgtttaagca ggatttctgt catcatggac
      301 agcaccgaac gggtgaatcc ccgccagtct gcctctgcac cggtcagcat cgccgccata
      361 ttctgtgcaa taccgtcaaa ggtctgcgtg gcagcacttt taacctgcga aaaactgtcc
      421 gtcgcacttt ccgcccactc gccccagccg gacttcagcc cggccatcca gcttccacga
      481 agctgctcct ccgcagacct ggtgttcttc agtgccgatg tggcctt
//
LOCUS       DABSYT020000424          521 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.430, whole genome shotgun sequence.
ACCESSION   DABSYT020000424 DABSYT020000000
VERSION     DABSYT020000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.430"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..95
                     /locus_tag="ID725_RS27395"
                     /old_locus_tag="pgaptmp_005479"
     CDS             <1..95
                     /locus_tag="ID725_RS27395"
                     /old_locus_tag="pgaptmp_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853829.1"
                     /translation="RSSLKENTETVEMNSKVWGRLKKIITFFKK"
     gene            complement(171..473)
                     /gene="bamE"
                     /locus_tag="ID725_RS27400"
                     /old_locus_tag="pgaptmp_005480"
     CDS             complement(171..473)
                     /gene="bamE"
                     /locus_tag="ID725_RS27400"
                     /old_locus_tag="pgaptmp_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGV7853830.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVRTYIFNAGTSNVKTGIF"
BASE COUNT          145 a          100 c          109 g          167 t
ORIGIN      
        1 taagatcctc tttaaaggaa aatactgaga ctgtagaaat gaacagtaaa gtttggggga
       61 ggctgaaaaa gataatcact ttctttaaaa agtgatttat gtttctgtaa aaagcccgta
      121 ggtatcaact gcgggctaaa ttttcccttg atgctggttt tgtcttttat ctaaaaaatg
      181 ccagtcttca cgttgcttgt tccagcatta aagatgtatg ttctgacttt ttcgcctttg
      241 aaagaaactg tcagagattt agtttgtgag tctgcgccac ctgcaagcag tcccacaacg
      301 gggatgaaag aggttgcttt ggactggctg ttatacatag tgtatgacca ttgttcttca
      361 ccatcgatca aagaacggct gtcaggttca ccgaacgatg ctaacacgtc ctgttttgta
      421 gttttgcctt taacaatttt ggttttcaca ctttcctgcg tctcattttt caatgactgg
      481 ttgcctactg aagaacaacc tgccaaggtc agaaaaacga a
//
LOCUS       DABSYT020000425          521 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.431, whole genome shotgun sequence.
ACCESSION   DABSYT020000425 DABSYT020000000
VERSION     DABSYT020000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.431"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..134)
                     /locus_tag="ID725_RS27405"
                     /old_locus_tag="pgaptmp_005481"
     CDS             complement(<1..134)
                     /locus_tag="ID725_RS27405"
                     /old_locus_tag="pgaptmp_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HGV7853831.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     "
     gene            complement(138..>521)
                     /locus_tag="ID725_RS27410"
                     /old_locus_tag="pgaptmp_005482"
     CDS             complement(138..>521)
                     /locus_tag="ID725_RS27410"
                     /old_locus_tag="pgaptmp_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGV7853832.1"
                     /translation="RLAGAHDFIRELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTN
                     PRILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFI
                     SEDGTHKELISKKDSLYAYLYQLQA"
BASE COUNT          158 a          112 c           83 g          168 t
ORIGIN      
        1 tgagcaggaa gaaatgcata ctcatctttt tctctcacag gagcatcaag tgtgtgacga
       61 attttccaga catcagaaaa aaccgtctta taacggcaaa ccaaatccca taaacctttc
      121 atgtaaaagc gcatacgtta tgcctgcaac tgatataaat atgcatataa actgtctttt
      181 ttggagataa gctctttatg tgtgccatct tcagaaataa agccattatc cataacaatt
      241 attctgtttg cattttttac agttgacaac ctgtgtgcaa taataattac ggttctgttc
      301 ttacatattc ttgacatatt tttcattatt atattttccg actcataatc aagagcactg
      361 gttgcttcat caaaaataag aattcgagga tttgtaacaa gagcccgggc tattgcgatc
      421 cgttggcgct gccctcctga aagaccaacg ccctgttctc caacaatagt attgtaccct
      481 tcttttagtt ctctaataaa atcatgggca cctgcaagtc t
//
LOCUS       DABSYT020000426          508 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.432, whole genome shotgun sequence.
ACCESSION   DABSYT020000426 DABSYT020000000
VERSION     DABSYT020000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.432"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>508
                     /locus_tag="ID725_RS27415"
                     /old_locus_tag="pgaptmp_005483"
     CDS             <1..>508
                     /locus_tag="ID725_RS27415"
                     /old_locus_tag="pgaptmp_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HGV7853833.1"
                     /translation="CHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDL
                     ISGWEKRQVMKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRVTHMSRQV
                     TTDTDTDKELNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFPMTGVWRP
                     SLDFRQRAA"
BASE COUNT          140 a          118 c          155 g           95 t
ORIGIN      
        1 atgtcacggc gagtcactac gtggtcacat tgatgtcacg acggaagatt tagcaagtgc
       61 gcttgatgtg acggaagacg tgattgattc aattttgcat gcaatgcagg ggcgagttct
      121 ggatggcgat cttatttccg gatgggaaaa acgccaggtg atgaaggagg ataacggtaa
      181 tgtttcgcaa accgcaaaat ccccggcaga gcgcaagaga gcgcagcggg agcgggaaag
      241 actgcgggag cagaacactg attgtcacga tgagtcacga cgcgtcacgc atatgtcacg
      301 acaagtcacg acagatacag atacagataa agaattaaac cccacacata acgcgcgcat
      361 gcgcgagagt gctccagcca gtgagtcgaa tggcgcgccg ttgcagacag cagaacctga
      421 atacccggat ggcctgagcg aaccgatcgg gaaatttccg atgactggtg tctggcggcc
      481 gtcgctggat tttcgacagc gggctgca
//
LOCUS       DABSYT020000427          471 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.433, whole genome shotgun sequence.
ACCESSION   DABSYT020000427 DABSYT020000000
VERSION     DABSYT020000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.433"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..203)
                     /locus_tag="ID725_RS27420"
                     /old_locus_tag="pgaptmp_005484"
     CDS             complement(<1..203)
                     /locus_tag="ID725_RS27420"
                     /old_locus_tag="pgaptmp_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGV7853834.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(355..>471)
                     /locus_tag="ID725_RS27425"
                     /old_locus_tag="pgaptmp_005485"
     CDS             complement(355..>471)
                     /locus_tag="ID725_RS27425"
                     /old_locus_tag="pgaptmp_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGV7853835.1"
                     /translation="AGVQFNPTESVTIDLAYEGSGSGDWRSDAFIVGIGYRF"
BASE COUNT          125 a          133 c           98 g          115 t
ORIGIN      
        1 ggaaatccct ccaccaacag aatgacgctg tactgaccgt actcaacatc catgctgtaa
       61 cgtccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtt
      121 cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc atctttaagc
      181 acgccagaaa tttttactgt catacttttc caccaataaa aaaagcccgc agcagtgacg
      241 ccacgggctt caggacagtg taactttacg tttcctcaaa cgcagttcac cccataaggt
      301 ggatgaacct gcgtatcata acaatattta cagaagataa atcggcgtct gttgtcagaa
      361 acggtatccg ataccaacaa taaatgcatc cgatcgccag tcgccactac cggaaccttc
      421 ataagcaagg tcaatggtca cggattcggt cgggttaaac tgcacgccag c
//
LOCUS       DABSYT020000428          457 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.434, whole genome shotgun sequence.
ACCESSION   DABSYT020000428 DABSYT020000000
VERSION     DABSYT020000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.434"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>457
                     /locus_tag="ID725_RS27430"
                     /old_locus_tag="pgaptmp_005486"
     rRNA            <1..>457
                     /locus_tag="ID725_RS27430"
                     /old_locus_tag="pgaptmp_005486"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          126 a          106 c          139 g           86 t
ORIGIN      
        1 agacccgaaa cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga
       61 ggaccgaacc gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc
      121 aatcaaaccg ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt
      181 catctccggg ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg
      241 caaactgcga ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt
      301 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga
      361 aacgatgtgg gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag
      421 aaagcgtaat agctcactgg tcgagtcggc ctgcgcg
//
LOCUS       DABSYT020000429          449 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.435, whole genome shotgun sequence.
ACCESSION   DABSYT020000429 DABSYT020000000
VERSION     DABSYT020000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.435"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>449
                     /gene="espX1"
                     /locus_tag="ID725_RS27435"
                     /old_locus_tag="pgaptmp_005487"
     CDS             <1..>449
                     /gene="espX1"
                     /locus_tag="ID725_RS27435"
                     /old_locus_tag="pgaptmp_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HGV7853836.1"
                     /translation="EKSNLSYGYYFGCVLSNISCFESDLSNTIFSNGEINNLFIKKSN
                     IFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILI
                     PLVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLQSINESMFL"
BASE COUNT          164 a           84 c           74 g          127 t
ORIGIN      
        1 gaaaagagca acctttcata tgggtattat tttggttgtg tattatccaa tatatcatgt
       61 tttgaaagcg acttatcaaa caccatcttt tcaaatggag aaataaacaa cctcttcatt
      121 aaaaaaagca acatatttgg aacatcgttc actaatacca tgataaaaaa tttgcgctgt
      181 gaagatatta tgcctggccg atggaccact caacttgtca ataagcactt aggttacaga
      241 tacacaggag tattcaaaac cctggcaagt attgatgaca aaccgtcccg tttcgaaatt
      301 ttgataccat tggtacaaac tttagttcgc gacaatgtaa agctaaataa tgatgtgtat
      361 aaagaactga aaaagtttat gcacgattac gacaaaacat ccccagaaat gagaaaatac
      421 cttcagagca tcaacgagtc tatgttcct
//
LOCUS       DABSYT020000430          447 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.436, whole genome shotgun sequence.
ACCESSION   DABSYT020000430 DABSYT020000000
VERSION     DABSYT020000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.436"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..124
                     /locus_tag="ID725_RS27440"
                     /old_locus_tag="pgaptmp_005488"
     CDS             <1..124
                     /locus_tag="ID725_RS27440"
                     /old_locus_tag="pgaptmp_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGV7853837.1"
                     /translation="QGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          128 a           84 c          144 g           91 t
ORIGIN      
        1 ccaggggaac ccgatgccgg tgccttatgg tgaaatgctg gttggttcac gacggatatc
       61 ccaggacatc agcacccgtg atgagggcgg agacgggaaa gtggtggtta tcgggcgggg
      121 atgaaaataa aaaaatcccg cagagttagc ggagctgcgg gagagaacga tgaagattaa
      181 cgttatggag ttatttttca ggcatcaaaa aagtaatgca gcgtcattat tgcggctaca
      241 ggcaattgcc ggaaatgtga agagtttcag aaattttatt ccgtcatgac acaggcaccc
      301 tccggggtgc ctgttgtttt ctggcataaa cagattcaga catcagacag gagaggggga
      361 cagagtgggt aaagggggcg gcaaggggca cacgccggta gaggcaaagg acaatcttaa
      421 gtccacgcag atgatgagcg tgattga
//
LOCUS       DABSYT020000431          438 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.437, whole genome shotgun sequence.
ACCESSION   DABSYT020000431 DABSYT020000000
VERSION     DABSYT020000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.437"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..>438)
                     /locus_tag="ID725_RS27445"
                     /old_locus_tag="pgaptmp_005489"
     CDS             complement(<1..>438)
                     /locus_tag="ID725_RS27445"
                     /old_locus_tag="pgaptmp_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HGV7853838.1"
                     /translation="NNVAIGGIPAKKYLEHGLFSEYRQDGTIPYVVSTLNISGPDMIM
                     RQMKKYYKSLGRIGEVHIKDNKLSDVNFLGVYASSNKDNKSFNWLNPVSVGINDITPD
                     DESSWAVRNNDINKILFEKINCHVPEKLPTSLYYEIDSRSFFQG"
BASE COUNT          132 a           84 c           63 g          159 t
ORIGIN      
        1 cccctgaaaa aaagaccgcg aatcgatttc atagtatagg gaggtaggta gtttttcggg
       61 tacatggcag tttatttttt cgaatagaat tttatttata tcattatttc tgacagccca
      121 tgagctttca tcatctggtg taatatcatt aataccaaca ctcactggat ttagccagtt
      181 aaaactttta ttgtctttat ttgaacttgc atatacaccc aaaaaattaa catcactcaa
      241 tttattatct ttaatatgca cttcacctat gcgcccaaga gatttatagt attttttcat
      301 ttgtcgcata atcatgtctg ggccagatat atttaaagtg ctaaccacat aaggaattgt
      361 accgtcctgt ctatattcag aaaacaaacc gtgttctaaa tatttctttg caggtattcc
      421 tcctattgcc acattatt
//
LOCUS       DABSYT020000432          437 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.438, whole genome shotgun sequence.
ACCESSION   DABSYT020000432 DABSYT020000000
VERSION     DABSYT020000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.438"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            24..100
                     /locus_tag="ID725_RS27450"
                     /old_locus_tag="pgaptmp_005490"
     tRNA            24..100
                     /locus_tag="ID725_RS27450"
                     /old_locus_tag="pgaptmp_005490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ID725_RS27455"
                     /old_locus_tag="pgaptmp_005491"
     tRNA            143..218
                     /locus_tag="ID725_RS27455"
                     /old_locus_tag="pgaptmp_005491"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT          124 a           92 c          110 g          111 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg caagttgttc gtgagtctct caaattttcg
      361 caacacgatg atgaatcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      421 cacggtggat gccctgg
//
LOCUS       DABSYT020000433          432 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.439, whole genome shotgun sequence.
ACCESSION   DABSYT020000433 DABSYT020000000
VERSION     DABSYT020000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.439"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..250)
                     /locus_tag="ID725_RS27460"
                     /old_locus_tag="pgaptmp_005492"
     CDS             complement(<1..250)
                     /locus_tag="ID725_RS27460"
                     /old_locus_tag="pgaptmp_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGV7853839.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLIICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISP"
BASE COUNT          149 a           80 c           63 g          140 t
ORIGIN      
        1 caggactgat acaggataca actctggcaa tggtatcgtg aacaggaata ccattttcaa
       61 aatcaccata ttgcttcaaa aaatcgagat gtgtttcccc aaaatcctct atatcttccc
      121 agccttctgc accagaaata acggcgcaaa taatcaacag tagaatatcc gataatttat
      181 gttccacttt ccaggcttgt ctgtaatcgg ggataataga aatatgttcc atcaattttt
      241 taagttccat tttgttctcc ttaattatgt aaggagtatt tgatcatata taagcaataa
      301 aaaacagctt caggtaatga ggagtatctc aatttttaaa cataaaatgc gaattattta
      361 gtacaaaaag tgaacttcta atgatctttc cctgtctaaa atgcctatac ataacatggt
      421 tattcaatta tt
//
LOCUS       DABSYT020000434          423 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.440, whole genome shotgun sequence.
ACCESSION   DABSYT020000434 DABSYT020000000
VERSION     DABSYT020000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.440"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>423
                     /gene="wzx"
                     /locus_tag="ID725_RS27465"
                     /old_locus_tag="pgaptmp_005493"
     CDS             <1..>423
                     /gene="wzx"
                     /locus_tag="ID725_RS27465"
                     /old_locus_tag="pgaptmp_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HGV7853840.1"
                     /translation="SSLISKILSLLSLILTVSLTLPYLGQERFGVWMTITSLGAALTF
                     LDLGIGNALTNRIAHSFACGKNLKMSRQISGGLTLLAGLSFVITAICYITSGMIDWQL
                     VIKGINENVYAELQHSIKVFVIIFGLGIYSNGVQKVYMG"
BASE COUNT          130 a           59 c           87 g          147 t
ORIGIN      
        1 tcaagtttga ttagtaaaat actctcacta ctttctctta tattaactgt aagtttaact
       61 ttaccttatt taggacaaga gagatttggt gtatggatga ctattaccag tcttggtgct
      121 gctctgacat ttttggactt aggtatagga aatgcattaa caaacaggat cgcacattca
      181 tttgcgtgtg gcaaaaattt aaagatgagt cggcaaatta gtggtgggct cactttgctg
      241 gctggattat cgtttgtcat aactgcaata tgctatatta cttctggcat gattgattgg
      301 caactagtaa taaaaggtat aaacgagaat gtgtatgcag agttacaaca ctcaattaaa
      361 gtctttgtaa tcatatttgg acttggaatt tattcaaatg gtgtgcaaaa agtttatatg
      421 gga
//
LOCUS       DABSYT020000435          418 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.441, whole genome shotgun sequence.
ACCESSION   DABSYT020000435 DABSYT020000000
VERSION     DABSYT020000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.441"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(5..>418)
                     /locus_tag="ID725_RS27470"
                     /old_locus_tag="pgaptmp_005494"
     CDS             complement(5..>418)
                     /locus_tag="ID725_RS27470"
                     /old_locus_tag="pgaptmp_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGV7853841.1"
                     /translation="SASTGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTK
                     EATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRTEGINFNQNNHVVIQNDGTNGQA
                     GPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSRR"
BASE COUNT           76 a          158 c           96 g           88 t
ORIGIN      
        1 gttttcatcg cctgctcccc gataacatac cgccatcacg caactgcagc cggagctcat
       61 cctgcgcccc cttgcgggcc atgtcataca ccgccttcat cagctgcggc cccgcctgtc
      121 cgttggtgcc gtcgttctga atcaccacgt gattgttctg attaaaatta atgccttccg
      181 tccgccgcat ctgcgccgga cttccggcac cgcccacata acccccttcc gcatagcccc
      241 gcatcaggcg gtacaggttg ccgacgccaa tccggctggt tgcctccttc gtgaagacaa
      301 actccccgcg atgaacaatt cccgcaggtt catatttacc ccccgtcccc gtaaatcccc
      361 cggtcgcgaa atggaagttc gccgccgcag cctgaatggc cgtccccgtg gaggcaga
//
LOCUS       DABSYT020000436          410 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.442, whole genome shotgun sequence.
ACCESSION   DABSYT020000436 DABSYT020000000
VERSION     DABSYT020000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.442"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            85..>410
                     /locus_tag="ID725_RS27475"
                     /old_locus_tag="pgaptmp_005495"
     CDS             85..>410
                     /locus_tag="ID725_RS27475"
                     /old_locus_tag="pgaptmp_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853842.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNTKTTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDSLAQMQYWTAVRNIIDDTNEVTIDLSYNLAIT
                     NIDTSD"
BASE COUNT          153 a           84 c           55 g          118 t
ORIGIN      
        1 tttcttaatc ttgattataa caagctagca tcgctggatg tctcccggca agagtctctg
       61 attgagttaa gtgcccatca caatatgatc actgacctta tattacacaa tcatcccagg
      121 atgaaaacaa tcactttaaa cgacaaccat attgcacatt taaacaccaa aactactaca
      181 aaacttgaat atttaaactt aagcaataac aatttattgc caaccaatga cattgatcaa
      241 ctaatatcat caaaacatct ttggcatgta ttagttaacg gcatcaacaa tgattcactt
      301 gcacaaatgc agtactggac tgcagtaaga aatataattg atgacactaa tgaagtgacc
      361 attgatttat cttataatct ggcaatcaca aatatcgata ccagtgatga
//
LOCUS       DABSYT020000437          400 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.443, whole genome shotgun sequence.
ACCESSION   DABSYT020000437 DABSYT020000000
VERSION     DABSYT020000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.443"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..169
                     /locus_tag="ID725_RS27480"
                     /old_locus_tag="pgaptmp_005496"
     CDS             <1..169
                     /locus_tag="ID725_RS27480"
                     /old_locus_tag="pgaptmp_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853843.1"
                     /translation="TEEQAEWLIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPR
                     PSRTFQQRIWR"
     gene            171..>400
                     /locus_tag="ID725_RS27485"
                     /old_locus_tag="pgaptmp_005497"
     CDS             171..>400
                     /locus_tag="ID725_RS27485"
                     /old_locus_tag="pgaptmp_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853844.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAG"
BASE COUNT           90 a          113 c          110 g           87 t
ORIGIN      
        1 cactgaagaa caggccgaat ggctcattca gtgttaccgc aggcgcggat gcgaggttaa
       61 aaaagccctt agcctcgact accgtcactg gataatctcc gtcaggctcc cttactccga
      121 acggccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaat gtgcgggtat
      181 tacttcgacc tgttctggta ccggaactcg gtctggttat cgttaagcca ggccgtgaat
      241 caatgtcagc attccataac ggcagaatac tggtggagcc ggaaccaaaa agcatgcgag
      301 ctctgccgtc cggggttgta cctgccgttc accagccgct ggcggaagat aaatcactac
      361 tgccattttt cagcgatgag cgggtgatcc gtgctgcggg
//
LOCUS       DABSYT020000438          398 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.444, whole genome shotgun sequence.
ACCESSION   DABSYT020000438 DABSYT020000000
VERSION     DABSYT020000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.444"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(84..293)
                     /gene="fumD"
                     /locus_tag="ID725_RS27490"
                     /old_locus_tag="pgaptmp_005498"
     CDS             complement(84..293)
                     /gene="fumD"
                     /locus_tag="ID725_RS27490"
                     /old_locus_tag="pgaptmp_005498"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HGV7853845.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          116 a           97 c           62 g          123 t
ORIGIN      
        1 agcagttgtt atttagaatg attaactgga ccgcaactga agcaagaata tcaggatgaa
       61 gcttatcgcc tcatcctgaa tggtcatttt accagtgcgt taatgacggt ttccatcgcc
      121 tgcttttcca gctcactgcg ctgaatgcgt ttattggcta atgctgtacg taatacgcct
      181 gcgataacaa tatgctttgg ttcctgcccc agatcgcgca tttccagcac aactttaccg
      241 acaactctgc acatttctcg gtacagttca tcctcttttg tccgatttcc cattctttat
      301 acctatttat catgccaact atcagcatat atcaatctaa ccaattaaac aaaaatcaaa
      361 caaaatcaga caaataacgc gataaattat tttaactg
//
LOCUS       DABSYT020000439          392 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.445, whole genome shotgun sequence.
ACCESSION   DABSYT020000439 DABSYT020000000
VERSION     DABSYT020000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.445"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(1..>392)
                     /locus_tag="ID725_RS27495"
                     /old_locus_tag="pgaptmp_005499"
     CDS             complement(1..>392)
                     /locus_tag="ID725_RS27495"
                     /old_locus_tag="pgaptmp_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGV7853846.1"
                     /translation="EVDVFIKIFESWVTKLPSISGPVNLHIPTSFKDKSLEVESYFVD
                     KSIWNVHIAYHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNHCFSPDKVNEICEQA
                     RHYLVEKMCETHSLAMNNSVLTNPEDS"
BASE COUNT          136 a           69 c           60 g          127 t
ORIGIN      
        1 ctatgagtcc tcagggtttg tcaaaacaga attattcata gccaaagaat gtgtttcaca
       61 cattttttca actaaataat gcctggcttg ttcgcagatt tcgttaacct tgtcagggga
      121 gaaacaatga ttattaatta aatattgctc acaattatcc acaaattcct gcggactaaa
      181 ttcagcaata aattgatcgg taaaaaacac aaaacgctta tcatcatgat atgcaatatg
      241 gacattccat attgacttgt cgacaaaata tgactctact tcaagagact tatctttgaa
      301 actggtaggt atatggagat ttacaggacc tgatattgat ggtaattttg ttacccaact
      361 ttcaaaaatt ttgataaaaa catcaacttc tt
//
LOCUS       DABSYT020000440          385 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.447, whole genome shotgun sequence.
ACCESSION   DABSYT020000440 DABSYT020000000
VERSION     DABSYT020000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.447"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..116
                     /locus_tag="ID725_RS27500"
                     /old_locus_tag="pgaptmp_005500"
     CDS             <1..116
                     /locus_tag="ID725_RS27500"
                     /old_locus_tag="pgaptmp_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGV7853847.1"
                     /translation="NRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSKKC"
     gene            complement(198..>385)
                     /locus_tag="ID725_RS27505"
                     /old_locus_tag="pgaptmp_005501"
     CDS             complement(198..>385)
                     /locus_tag="ID725_RS27505"
                     /old_locus_tag="pgaptmp_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGV7853848.1"
                     /translation="NIRFPNQMIEQINIALDQKGSGNFSAWVIEACRRRLINEKHAQF
                     VPNKDKHDQSTCSDRFT"
BASE COUNT          113 a           65 c           90 g          117 t
ORIGIN      
        1 tcaacagact acattctgag ttgggaatgg gatttgaaga gcggcgggta ttccgacgat
       61 ataaagggac aggagaacag aatacgtccg gaaacgcgcg gtcaaaaaaa tgctaaaaaa
      121 tatctgagag cgttattgcc tgttaccata agaaaaagcg actttagtgg tcgctttttg
      181 tgtcatatat aagtcgttta agtaaacctg tctgaacagg tgctctggtc gtgtttgtct
      241 ttgttgggta caaattgagc atgtttttca ttaattaatc ttcttctgca ggcttcaata
      301 acccacgctg aaaaattacc tgaacctttc tgatcaagag cgatgttaat ttgttcaatc
      361 atttggttag gaaagcggat gttgc
//
LOCUS       DABSYT020000441          384 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.448, whole genome shotgun sequence.
ACCESSION   DABSYT020000441 DABSYT020000000
VERSION     DABSYT020000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.448"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..>384)
                     /locus_tag="ID725_RS27510"
                     /old_locus_tag="pgaptmp_005502"
     CDS             complement(<1..>384)
                     /locus_tag="ID725_RS27510"
                     /old_locus_tag="pgaptmp_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGV7853849.1"
                     /translation="ASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSS
                     WVLEYAAGNGAALLQKGGQIRCRFKVSGALAANQYVMAFYWPVSSLPQGVALTGDGGN
                     NLLAAFYIQTDAKDLNVMYHNAKVA"
BASE COUNT           68 a          138 c           85 g           93 t
ORIGIN      
        1 tcgccacttt cgcattgtgg tacatcacat tcaggtcttt tgcatctgtc tggatgtaga
       61 acgctgccag caggttattc cccccgtctc cggtcagggc aacgccctgt ggcagtgaag
      121 ataccggcca gtaaaacgcc ataacatact ggttcgcagc cagcgctccc gaaaccttaa
      181 agcggcagcg aatctgcccc cctttctgta acagagccgc accgttgccc gcggcgtact
      241 ccagcaccca gctgctttta ccggcttcct tggtcagctt cactgcctta cctccggttc
      301 cctccgcatc gctgaccact tctgccctgc cgccactggc tgaccatccc tgtactttca
      361 ggcttccctc tgactcgctg gcaa
//
LOCUS       DABSYT020000442          368 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.449, whole genome shotgun sequence.
ACCESSION   DABSYT020000442 DABSYT020000000
VERSION     DABSYT020000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.449"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            complement(<1..260)
                     /locus_tag="ID725_RS27515"
                     /old_locus_tag="pgaptmp_005503"
     CDS             complement(<1..260)
                     /locus_tag="ID725_RS27515"
                     /old_locus_tag="pgaptmp_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGV7853850.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLRQYGDFENGIPVHDTIARVVSCISPAKF"
BASE COUNT          121 a           67 c           60 g          120 t
ORIGIN      
        1 tgaaattttg caggactgat acaggataca actctggcaa tggtatcgtg gacaggaata
       61 ccattttcaa aatcaccata ttgcctcaaa aaatcgagat gcgtttcacc aaaatcctct
      121 atatcttccc aaccttctgc accagaaatg acggcacaaa tagtcaacag tagaatatct
      181 gacaatttat gttctacttt ccaggcttgt ctgtaatcgg ggataataga aatatgttcc
      241 atcaattttt taagttccat tttgttctcc ttatttatgc aaggagtatt tgatcatatg
      301 taagctgtga aaaacagttt tagataataa ggaacatctc aattattgaa catttagtgc
      361 gaattatt
//
LOCUS       DABSYT020000443          365 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.450, whole genome shotgun sequence.
ACCESSION   DABSYT020000443 DABSYT020000000
VERSION     DABSYT020000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.450"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..264
                     /locus_tag="ID725_RS27520"
                     /old_locus_tag="pgaptmp_005504"
     CDS             <1..264
                     /locus_tag="ID725_RS27520"
                     /old_locus_tag="pgaptmp_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGV7853851.1"
                     /translation="AVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLGIHLKQAGSDFK
                     LHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            261..>365
                     /locus_tag="ID725_RS27525"
                     /old_locus_tag="pgaptmp_005505"
     CDS             261..>365
                     /locus_tag="ID725_RS27525"
                     /old_locus_tag="pgaptmp_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HGV7853852.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGK"
BASE COUNT           96 a           85 c           97 g           87 t
ORIGIN      
        1 gctgtccgtg accctcaaat ggaaatgacg ttgacctgga gtggtacgca gagtgacttt
       61 gacgattcgg ccgtagccac ggatctcaat ggattaggaa tacacctcaa acaagccggg
      121 agcgatttta agctgcatac gcccttagtg gtgaatgaaa catcccttcc ggtactaacg
      181 gcagtacccg taaaaaagag cggtgtcgac ttacccgagt cagactttga agcatgggct
      241 acgttacagg tggattacca atgaagaata accgtgcgtg ggcgcttatc agtggtctga
      301 tattgttcag cggaacggcc ccagctgccg ataacctgca ttttaccggt aatttgcttg
      361 gtaaa
//
LOCUS       DABSYT020000444          354 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.451, whole genome shotgun sequence.
ACCESSION   DABSYT020000444 DABSYT020000000
VERSION     DABSYT020000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.451"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>354
                     /locus_tag="ID725_RS27530"
                     /old_locus_tag="pgaptmp_005506"
     CDS             <1..>354
                     /locus_tag="ID725_RS27530"
                     /old_locus_tag="pgaptmp_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGV7853853.1"
                     /translation="NIKLTLREYADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAAT
                     KPLIITFPALVTTLHDSMRPLTSSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQ
                     ALASVSAGQNDYFIG"
BASE COUNT          112 a           86 c           60 g           96 t
ORIGIN      
        1 aaatatcaaa ttaacactcc gggaatacgc agatcatcaa aaagcaatgg acgcgctgga
       61 agaaggtgaa gtcgatatag tgttatcaca tttagttgct tcgccgcctc ttaatgatga
      121 cattgctgca accaaaccac tgataattac ctttccggcg ctggtcacca cccttcacga
      181 ttcaatgcga ccgcttacct catcaaaacc agtaaatatt gctcgagtag caaattatcc
      241 ccctgacgag gtaattcatc aatcatttcc aaaagcaaca attatctctt ttacaaattt
      301 atatcaggca ttagcatccg tctcagccgg acagaatgat tactttattg gtag
//
LOCUS       DABSYT020000445          344 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.452, whole genome shotgun sequence.
ACCESSION   DABSYT020000445 DABSYT020000000
VERSION     DABSYT020000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.452"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            104..>344
                     /locus_tag="ID725_RS27535"
                     /old_locus_tag="pgaptmp_005507"
     CDS             104..>344
                     /locus_tag="ID725_RS27535"
                     /old_locus_tag="pgaptmp_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGV7853854.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPP"
BASE COUNT           67 a           76 c          128 g           73 t
ORIGIN      
        1 aacggacaga tagggccgga ggcggcaggc agtcttgtgc agctgattga ccagcgggtg
       61 caggcggtga tgctgtccat gcgacgtgac ggaggaatgc tgagtggctg agataaaaac
      121 gctgcatctg gtcccgcgtg aagggatgca ggtgagtgag aagccgtcgg tggtgagggt
      181 gcggtttggt gacggttatg aacagcgccg ccccacaggg ctgaatcctc aactgaagac
      241 gtttcaggcg gtgttccggg tgacggatga gtcaacccgg cgctggctgg atgaattttt
      301 atcctggcat ggtggttacc gtgccttttt gtggcgaccg ccga
//
LOCUS       DABSYT020000446          342 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.453, whole genome shotgun sequence.
ACCESSION   DABSYT020000446 DABSYT020000000
VERSION     DABSYT020000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.453"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>342
                     /locus_tag="ID725_RS27540"
                     /old_locus_tag="pgaptmp_005508"
     CDS             <1..>342
                     /locus_tag="ID725_RS27540"
                     /old_locus_tag="pgaptmp_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGV7853855.1"
                     /translation="GQNTELAKLKYQTALGELKTLTEMQKQELLRNATLIDQQKIREQ
                     LRSREETLKNENAAARASNDAELLGYGQGERARERMRELQQIRDSFRQKDADLQSQYQ
                     TGDISEDFYRQA"
BASE COUNT           97 a           78 c          115 g           52 t
ORIGIN      
        1 ggggcagaat acagagctgg cgaagctgaa ataccagaca gcgctgggtg aactgaaaac
       61 cctgacggag atgcagaagc aggaactgct gcgtaacgcg accctgattg accagcaaaa
      121 aatccgggaa cagttgcgat cccgggaaga gacactgaag aatgagaatg cggctgcgcg
      181 tgcgtcgaat gatgctgaac tgctggggta cgggcagggg gagcgagcca gagaacgcat
      241 gcgggagttg cagcagatcc gcgacagctt ccgccagaag gatgcggacc ttcagtctca
      301 gtatcagacc ggggatatca gtgaggattt ttacagacag gc
//
LOCUS       DABSYT020000447          341 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.454, whole genome shotgun sequence.
ACCESSION   DABSYT020000447 DABSYT020000000
VERSION     DABSYT020000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.454"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>341
                     /locus_tag="ID725_RS27545"
                     /old_locus_tag="pgaptmp_005509"
     CDS             <1..>341
                     /locus_tag="ID725_RS27545"
                     /old_locus_tag="pgaptmp_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGV7853856.1"
                     /translation="GRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGD
                     GFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRRVALIRQ
                     QAAEQNKVYQS"
BASE COUNT           91 a           81 c          110 g           59 t
ORIGIN      
        1 aaggacgaac acgggcggcg ctgacagagc agaaccggct gattgatgaa caggccagtc
       61 gggtgaaatc cctgcaggaa aaaatcgcag gatatcagta tgttctggcg aacccgggct
      121 ggacgaccgg tgacggattc atgataaacc atctgacatc ggtgaagacc gtaacggaag
      181 ggcttgctca ggcaacagag cagcttgccg ttgagcagtc ccgtctggca cagatgcagg
      241 aaaaagcgca gtccattcag gatgtgcttg ccgggctgga agaccgtcgt gtggcgttaa
      301 ttcgtcagca ggcggcagag cagaataagg tgtaccagtc c
//
LOCUS       DABSYT020000448          338 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.455, whole genome shotgun sequence.
ACCESSION   DABSYT020000448 DABSYT020000000
VERSION     DABSYT020000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.455"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>338
                     /gene="nleB2"
                     /locus_tag="ID725_RS27550"
                     /old_locus_tag="pgaptmp_005510"
     CDS             <1..>338
                     /gene="nleB2"
                     /locus_tag="ID725_RS27550"
                     /old_locus_tag="pgaptmp_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HGV7853857.1"
                     /translation="EISKAQDSLISMYAKKRNHAWFDFFRNLALLKAGEIFRCTYNTK
                     NHGISFGEGCIYLDMDMILTGKLGTIYAPDGISMHVDRRNDSVNIENSAIIVNRSNHP
                     ALLEGLSFMH"
BASE COUNT          108 a           46 c           72 g          112 t
ORIGIN      
        1 ggaaatttca aaggcgcagg attctttgat ttctatgtat gcaaagaaaa gaaatcatgc
       61 atggtttgat ttcttcagaa atttagcctt attaaaagca ggagagatat tcaggtgcac
      121 atataataca aagaatcacg gtatttcatt cggggagggg tgtatctatc ttgatatgga
      181 tatgatactt acaggtaagc ttggtacaat atatgctcct gatggaattt caatgcatgt
      241 ggatcgtcgt aatgatagtg taaatattga aaatagtgca ataattgtta accgtagtaa
      301 tcatcctgct ctacttgagg gactttcttt tatgcata
//
LOCUS       DABSYT020000449          333 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.456, whole genome shotgun sequence.
ACCESSION   DABSYT020000449 DABSYT020000000
VERSION     DABSYT020000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.456"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..>333
                     /locus_tag="ID725_RS27555"
                     /old_locus_tag="pgaptmp_005511"
     CDS             <1..>333
                     /locus_tag="ID725_RS27555"
                     /old_locus_tag="pgaptmp_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGV7853858.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAK"
BASE COUNT           74 a           85 c          103 g           71 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaa
//
LOCUS       DABSYT020000450          329 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.denovo.457, whole genome shotgun sequence.
ACCESSION   DABSYT020000450 DABSYT020000000
VERSION     DABSYT020000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.denovo.457"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..313
                     /locus_tag="ID725_RS27560"
                     /old_locus_tag="pgaptmp_005512"
     CDS             <1..313
                     /locus_tag="ID725_RS27560"
                     /old_locus_tag="pgaptmp_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGV7853859.1"
                     /translation="AFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFLF
                     FGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPA
                     E"
BASE COUNT           75 a           89 c           96 g           69 t
ORIGIN      
        1 ggcgttcgcg tacgcactta accagtggcc ggcactgacg tactatgcga acgatggctg
       61 ggtggaaatc gacaacaaca tcgctgaaaa tgccctgcgg gcggtcagtc tgggtcgtaa
      121 aaacttcctg ttcttcggct ctgatcatgg tggtgagcgg ggagcgctac tgtacagcct
      181 gatcgggacg tgcaaactga atgacgtgga tccagaaagc taccttcgcc atgtgcttgg
      241 cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact
      301 gccagctgaa taacacatcc ccgtcaata
//
LOCUS       DABSYT020000451         8186 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.guided.156, whole genome shotgun sequence.
ACCESSION   DABSYT020000451 DABSYT020000000
VERSION     DABSYT020000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.guided.156"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            333..2141
                     /gene="frdA"
                     /locus_tag="ID725_RS27565"
                     /old_locus_tag="pgaptmp_005513"
     CDS             333..2141
                     /gene="frdA"
                     /locus_tag="ID725_RS27565"
                     /old_locus_tag="pgaptmp_005513"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGV7853860.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2134..2868
                     /gene="frdB"
                     /locus_tag="ID725_RS27570"
                     /old_locus_tag="pgaptmp_005514"
     CDS             2134..2868
                     /gene="frdB"
                     /locus_tag="ID725_RS27570"
                     /old_locus_tag="pgaptmp_005514"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGV7853861.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2879..3274
                     /gene="frdC"
                     /locus_tag="ID725_RS27575"
                     /old_locus_tag="pgaptmp_005515"
     CDS             2879..3274
                     /gene="frdC"
                     /locus_tag="ID725_RS27575"
                     /old_locus_tag="pgaptmp_005515"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGV7853862.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3285..3644
                     /gene="frdD"
                     /locus_tag="ID725_RS27580"
                     /old_locus_tag="pgaptmp_005516"
     CDS             3285..3644
                     /gene="frdD"
                     /locus_tag="ID725_RS27580"
                     /old_locus_tag="pgaptmp_005516"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGV7853863.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            3707..4840
                     /locus_tag="ID725_RS27585"
                     /old_locus_tag="pgaptmp_005517"
     CDS             3707..4840
                     /locus_tag="ID725_RS27585"
                     /old_locus_tag="pgaptmp_005517"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGV7853864.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            4928..5461
                     /gene="blc"
                     /locus_tag="ID725_RS27590"
                     /old_locus_tag="pgaptmp_005518"
     CDS             4928..5461
                     /gene="blc"
                     /locus_tag="ID725_RS27590"
                     /old_locus_tag="pgaptmp_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGV7853865.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5458..5775)
                     /gene="sugE"
                     /locus_tag="ID725_RS27595"
                     /old_locus_tag="pgaptmp_005519"
     CDS             complement(5458..5775)
                     /gene="sugE"
                     /locus_tag="ID725_RS27595"
                     /old_locus_tag="pgaptmp_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGV7853866.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5951..6097)
                     /gene="ecnB"
                     /locus_tag="ID725_RS27600"
                     /old_locus_tag="pgaptmp_005520"
     CDS             complement(5951..6097)
                     /gene="ecnB"
                     /locus_tag="ID725_RS27600"
                     /old_locus_tag="pgaptmp_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGV7853867.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6208..6333)
                     /gene="ecnA"
                     /locus_tag="ID725_RS27605"
                     /old_locus_tag="pgaptmp_005521"
     CDS             complement(6208..6333)
                     /gene="ecnA"
                     /locus_tag="ID725_RS27605"
                     /old_locus_tag="pgaptmp_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGV7853868.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(6385..6951)
                     /gene="efp"
                     /locus_tag="ID725_RS27610"
                     /old_locus_tag="pgaptmp_005522"
     CDS             complement(6385..6951)
                     /gene="efp"
                     /locus_tag="ID725_RS27610"
                     /old_locus_tag="pgaptmp_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGV7853869.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6993..8021
                     /gene="epmB"
                     /locus_tag="ID725_RS27615"
                     /old_locus_tag="pgaptmp_005523"
     CDS             6993..8021
                     /gene="epmB"
                     /locus_tag="ID725_RS27615"
                     /old_locus_tag="pgaptmp_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGV7853870.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2043 a         2098 c         2131 g         1914 t
ORIGIN      
        1 tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg caacgcgacg
       61 gcgagacaaa ttttacgcag gaataaaaca acggtgggca gtgactaaaa aaagcacgat
      121 ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc ttgcgcacta
      181 aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt gtacctataa
      241 aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct gaagtacggg
      301 gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca
      361 ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa
      421 atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc gttgctgcag
      481 aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac tttcacgata
      541 cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc gtccaccact
      601 gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt cgcccggatg
      661 gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgtacctgg ttcgccgccg
      721 ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga
      781 tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt catgttcgcg
      841 gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct aacgcggtcg
      901 ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc ggcatcgtta
      961 ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac atggaattcg
     1021 ttcagtatca tccaaccggt ctgccaggtt ccggtatcct gatgaccgaa ggttgccgcg
     1081 gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat tacggcatgg
     1141 gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt ccacgcgaca
     1201 aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc acgccgcgtg
     1261 gtgatgtggt ttatctcgac ctgcgtcacc tcggcgagaa aaaactgcat gaacgtctgc
     1321 cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc
     1381 cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag aactgtgaaa
     1441 cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg cacggtgcaa
     1501 accgtctggg ctccaactcc ctggcggaac tggtggtctt cggccgtctg gccggtgaac
     1561 aagcgacaga gcgtgcagca actgccggta atggcaacga agcggcaatt gaagcgcagg
     1621 cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc gaaaactggg
     1681 cgaagatccg cgacgaaatg ggcctggcta tggaagaagg ttgcggtatc taccgtacgc
     1741 cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc ttcaagcgcg
     1801 tgcgcatcac cgacacttcc agcgtgttca acaccgacct gctctacacc attgaactgg
     1861 gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt aaagagtccc
     1921 gcggcgcaca ccagcgtctg gacgaaggtt gcaccaagcg tgacgacgtc aacttcctca
     1981 aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac agcgacgtga
     2041 agattactac gctaccgcca gctaaacgcg tttacggtgg cgaagcggat gcagccgata
     2101 aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt
     2161 gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg
     2221 ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga caacctggca
     2281 ccggacctga gctaccgctg gtcctgccgt atggcgattt gtggttcctg cggcatgatg
     2341 gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac cgacggtatg
     2401 aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga tatgacccac
     2461 ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg caccgcggat
     2521 cagggtacta acatccagac cccggcgcag atggcgaagt atcaccagtt ctccggttgc
     2581 atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc
     2641 ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga ccacggtaag
     2701 aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac tttcgtgggc
     2761 tactgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca acagggcaaa
     2821 gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg agtgcaacat
     2881 gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga aaaaattgcc
     2941 gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat
     3001 tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gaagcctggg cgggattcgt
     3061 cgacttttta caaaacccgg ttattgtgat cattaacctg atcactctgg cggcagctct
     3121 gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca ttgtaaaaga
     3181 cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg tggttgccac
     3241 catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt aatccaaatc
     3301 caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt atgtggagcg
     3361 caattattgc gccggtgatg atcctgctgg tgggtattct gctgccgctg gggctgtttc
     3421 cgggtgatgc actgagctac gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg
     3481 tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg
     3541 cgatgcacga tctgaaaatc cacgtacctg cgggcaaatg ggttttctac ggtctggctg
     3601 ctatcctgac agttgtcacg ctgattggtg tcgttacaat ctaacgcatc gccaatgtaa
     3661 atccggcccg cctatggcgg gccgttttgt atggaaacca gaccctatgt tcaaaacgac
     3721 gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc aacaaatcaa
     3781 cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc ccggtatggc
     3841 ggtggcggta atttatcagg gtaaacctta ttactttacc tgggggtatg cggacatcgc
     3901 caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt
     3961 tacgggcgtg cttggtggcg acgctattgc tcgaggggaa atcaagttaa gcgatcccgc
     4021 aacaaaatac tggcctgaac ttaccgctaa acagtggaat gggatcacac tattacatct
     4081 cgcaacctac actgctggcg gcctgccatt gcaggtgccg gatgaagtga aatcctcaag
     4141 cgacttgctg cgcttctatc aaaactggca gcctgcatgg gctccaggaa cacaacgtct
     4201 gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt ctggtttgag
     4261 ttttgagcag gcgatgcaaa ctcgtgtctt ccagccactc aaactcaacc atacgtggat
     4321 taatgtaccg cccgcagaag aaaagaatta cgcctgggga tatcgcgaag gcaaggcagt
     4381 tcatgtttcg ccaggggcgt tagatgctga aacttatggt gtgaagtcga ccattgaaga
     4441 tatggcctgc tgggtacgaa gcaatatgaa tccccgtgat atcaacgaca aaacacttca
     4501 gcaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt atcagggtct
     4561 gggctgggaa atgctggact ggccggtaaa tcctgacatc atcgttaacg gcagtgacaa
     4621 taaaattgca ctggcagcac gccccgtaaa agcgattacg cctccaactc ctgcagtacg
     4681 agcatcatgg gtgcataaaa cgggggcgac cggcggattt ggtagctatg tcgcgtttat
     4741 tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca atccagcgag
     4801 agtcgccgcc gcctggcaga ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt
     4861 ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa ccagtaagga
     4921 aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc
     4981 ctgcagttct cctacgccgc cgcgtggcgt gaccgtagtg aataatttcg acgctaaacg
     5041 ctatctcggt acctggtatg agattgcccg ttttgatcac cgctttgaac gtggactgga
     5101 aaaagtcacc gcaacataca gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg
     5161 ctataaccct gacagaggaa tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc
     5221 accaactcgc gctgcgctga aagtgtcatt ctttggtcct ttctatggcg gttataacgt
     5281 tattgcactc gatcgggaat accgccatgc gctggtttgc ggaccggacc gcgactacct
     5341 gtggatactc tcccgcacgc caaccatttc tgacgaagtg aaacaggaga tgctggcagt
     5401 cgcgacccgg gaagggtttg atgtcagtaa gtttatttgg gtacagcagc ccggtagtta
     5461 gtgagtgctg agtttcagac caataatccc caatacgatt aacgccagac tcgccagacg
     5521 catcgggtta gcggactcac cgagcagcac aatgccggtg atggccgcgc cgactgcgcc
     5581 aatacccgtc cacacggcat aagccgtccc tactggtaac gatttcatcg cccaggcaag
     5641 tagcgccata ctgacaatca tcgccgtcac ggtaataaca ctcggcgtca aacgactaaa
     5701 gccgtgggta tatttcaggc ctacggccca taccacttcc agcagaccag caataactaa
     5761 gataatccag gacatatcag gctccggaga attggggccg tccccggtga aaagacgcgt
     5821 ttgcaggtcg tcctgcaaag ctgatggttg aacggatatt ttgccaggtg gcgggggaat
     5881 ttcaactatt acctttgtga ctatcgcaga aaaaacaaac ggcacgacac agctgtcgta
     5941 ccgttattgc ttattgctgc gctttcgttg ctgcaccaga aatcgcgtta ccgccatcag
     6001 aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca
     6061 gaacagaaaa gatcgctgca attgtcttct tcaccataac gtcttccttt taggtaatgt
     6121 tatttatgtt tgcctatagc aaattaataa tagacaacat cgccaaaaat gatggaaatc
     6181 tgatgatttt tcggaagaga agaaaaatta gctggcagcg cgggagatgg agttgccgag
     6241 atgtttgatg tcttcgccga aaccgcgagc ggtgttacag cccgtgagca gcgtgctggc
     6301 aagcaaaaca agaacgataa ggcgtttcat catctctgac ctgaataagt gatggtgcag
     6361 cctgcaggcc gcaccacaac cgcattactt cacgcgagag acgtattcac cagagcgggt
     6421 atccactttg atgacttcgc cgatttgtac gaacagcgga actttaacca cagcgccagt
     6481 agacagggta gccggtttgc cgccagtacc tgcggtatca cctttcaggc ccggatcggt
     6541 atcaacgatt tccagttcaa cgaagttcgg cggagtaacg gagatcggct gaccattcca
     6601 cagagttacg atacactctg cctgatccag cagccattta gcgttgtcgc caattgcttt
     6661 tgcgtcagca gacagctgct cgaaagtttc gttgttcatg aagtgccaga actcaccgtc
     6721 gttgtacagg taagtcaggt tcatatcgac aacatcagcg ccttcagcgg aatcagtaga
     6781 tttgaaggtt ttttctacgc gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa
     6841 tgcctggcct ttacccggtt ttacgaattc actcgcttca accgcgtaag gttcgccgtc
     6901 taacatgatt ttaagaccag cacgaaaatc gttgctatag tacgttgcca taaggccctc
     6961 tgaaatttgt taattggtag ctaagccaca aaatggcgca tattgtaacc ctaaataccc
     7021 catccagaga agattggtta acgcaacttg ccgatgttgt gaccgatcct gatgaacttc
     7081 tgcgtctttt gaatatagac gcggacgaaa aactgttggc cggacgcaac gccaaaaagc
     7141 tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat ggagaaaggc aatccggacg
     7201 atccgctttt gcgtcaggta cttacctcgc aagatgagtt tgtcgtcgcg cccggattct
     7261 ccaccgaccc gctggaagaa cagcacagcg tagtgcctgg tttgttgcat aaataccaca
     7321 accgggcgct tttgctggtc aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc
     7381 gccacttccc ctatgccgaa aatcagggca acaagcgtaa ctggcaaact gcacttgagt
     7441 atgttgctgc gcatccggaa ctggacgaga tgattttctc cggcggcgat ccgctgatgg
     7501 cgaaagatca cgagctggac tggttgctca cacaactgga agccatcccg catatcaaac
     7561 gtctgcggat tcacagtcgt ctgccgattg tgatcccggc acgcatcaca gatgcgctgg
     7621 ttgaacgctt ttcacattct acgctgcaaa tcttgctggt gaatcacatc aaccatgcca
     7681 atgagataga tgaaacattc cgtcaggcga tggctaagtt gcgccgggcg ggcgttactt
     7741 tgctgaacca gagcgttctg ttacgtggtg tgaacgataa cgcacaaacg ctggcaaacc
     7801 tgagtaatgc gttgttcgat gcaggcgtga tgccgtatta cctgcatgtg ctcgataaag
     7861 tacagggcgc ggcgcatttt atggtgagtg atgacgaagc acggcagatt atgcgtgagt
     7921 tgctgacact ggtgtcggga tatctggtgc cgaaactggc gcgagaaatt ggcggcgaac
     7981 ccagcaaaac gccgctggat ctccagctac gccagcagta actgattaaa cagggaagtt
     8041 taatctttgc ccgttacata acctttaatt attcatatac attgctgttg ttactcttta
     8101 tataaccaac accaataatt aacctttctt ttctgtttat atagagtgag tacggctatt
     8161 tatgttattt taacaactat tactac
//
LOCUS       DABSYT020000452         4239 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.guided.202, whole genome shotgun sequence.
ACCESSION   DABSYT020000452 DABSYT020000000
VERSION     DABSYT020000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.guided.202"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            <1..83
                     /gene="prfH"
                     /locus_tag="ID725_RS27620"
                     /old_locus_tag="pgaptmp_005524"
     CDS             <1..83
                     /gene="prfH"
                     /locus_tag="ID725_RS27620"
                     /old_locus_tag="pgaptmp_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGV7853871.1"
                     /translation="QRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(140..1597)
                     /gene="pepD"
                     /locus_tag="ID725_RS27625"
                     /old_locus_tag="pgaptmp_005525"
     CDS             complement(140..1597)
                     /gene="pepD"
                     /locus_tag="ID725_RS27625"
                     /old_locus_tag="pgaptmp_005525"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGV7853872.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            1858..2316
                     /gene="gpt"
                     /locus_tag="ID725_RS27630"
                     /old_locus_tag="pgaptmp_005526"
     CDS             1858..2316
                     /gene="gpt"
                     /locus_tag="ID725_RS27630"
                     /old_locus_tag="pgaptmp_005526"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGV7853873.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2408..3652
                     /gene="frsA"
                     /locus_tag="ID725_RS27635"
                     /old_locus_tag="pgaptmp_005527"
     CDS             2408..3652
                     /gene="frsA"
                     /locus_tag="ID725_RS27635"
                     /old_locus_tag="pgaptmp_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGV7853874.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            3710..4111
                     /gene="crl"
                     /locus_tag="ID725_RS27640"
                     /old_locus_tag="pgaptmp_005528"
     CDS             3710..4111
                     /gene="crl"
                     /locus_tag="ID725_RS27640"
                     /old_locus_tag="pgaptmp_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGV7853875.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4150..>4239)
                     /locus_tag="ID725_RS27645"
                     /old_locus_tag="pgaptmp_005529"
     CDS             complement(4150..>4239)
                     /locus_tag="ID725_RS27645"
                     /old_locus_tag="pgaptmp_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGV7853876.1"
                     /translation="DYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1020 a         1052 c         1101 g         1066 t
ORIGIN      
        1 cgcagcggcg aatgttccat caccagattg aacgtggcaa cccgcgacga acgttcacag
       61 ggatggcttt tatcgaagga taatgaagga tgaaactgcc ggaaggcgat taaacgccat
      121 ccggcagcaa atcaaataat tacttcgccg gaatttcttt cagcagttca gtcagcagtg
      181 tccagtaatg acctacgctt tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga
      241 ttgttggccc gatagaaacc atgtccattt ccggatacgg ttttttgaac agaccacatt
      301 ccaggcccgc gtggataatc tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt
      361 cacgtaccag atgcatcacc ggagaattag cgtccggctg ccagccagga tatgcgcctt
      421 tcgcttcggt tttcgcgcca gccagtttac ccagcgaatc cagcatgctc accacgtagt
      481 ctttaccgct gtcgatcaga gaacggatca gacagtgaat ttctacgtta ttgtcagtca
      541 tggtcaccac accgacgttc agggaggttt cgaccacacc ttttgccacg tcggagttgc
      601 ggatcacacc gttcggggtg gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa
      661 tcagggcagc tttatcgttc gctacagagt ccagcaacaa ggccagattt ttctctttct
      721 ctgccagctc gtttttcagg atctcctgat aggtattcac cagagatttc agggcgtcga
      781 ctttatcagc tgcgacagca atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg
      841 tgccgccgtt gaaatcaata agacgcaggt ccagttcttc tgcatgaccc gccaggaagc
      901 gcaccagcag tttgttggca ttacccaggc caacgtggat ttccccgccg gagtgaccgc
      961 ctttcagacc ttttaaggtt aacttgaagg tttcaaaacc agctggaacc gcttcacgat
     1021 ctaaatgcag gttggaggtg aagtcgatac cccccgcgca acccatgtag atttcacctt
     1081 cttcttcgga gtcggtgtta atcagaatat cagcctgcaa ccagttgctc tgtaagccga
     1141 acgcaccgtc cataccggct tcttcggtca tggtcagcag cacttccagc gggccgtgaa
     1201 ccacgttttc gtcagccaga accgccagtg cagaggccat accaatgccg ttatccgcac
     1261 ccagcgtggt gccgcgcgct ttaacccatt cgccatcaat atatggctgg ataggatctt
     1321 tcgtgaagtc atgcacggtg tcgttatttt tctgcggcac catatcgagg tgggcctgca
     1381 agacgaccgg tttacgattt tccatacctg cggtagcagg tttacgaatc aggatattac
     1441 ctacctgatc gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca atgtattcag
     1501 cgagttgctc ttcatgatag gacgggtgag gaatagaaca gattttggca aaaatatccc
     1561 acagcggctg tggagataat tgagacagtt cagacacgtt aagtctcctt gtcgatcacc
     1621 cgcaaaacag tattgcaggt cacagggtta gcagaaaatg ttgtcaacac aagacaggct
     1681 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     1741 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     1801 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     1861 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     1921 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta
     1981 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     2041 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     2101 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     2161 gaaatgtatc caaaagcgca ctttgtcacc atctttgcaa aaccggctgg tcgtccgctg
     2221 gttgatgatt atgttgttga tatcccgcaa gatacctgga tcgaacagcc gtgggatatg
     2281 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     2341 ggcgttgttc tttttaactt caggcgagtt acaatagttt ccagtaagta ttctggaggc
     2401 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     2461 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     2521 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     2581 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     2641 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     2701 ttatgagtgg gccacccagg cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc
     2761 gcaactcagt ggtcgtcact ggctgcatgc ggctacgttg tacaacattg ccgcctatcc
     2821 tcatctgaaa ggagatgacc tggccgagca agcgcaggct ttgtcaaacc gcgcctatga
     2881 agaggccgct cagcgtctac cgggcacgat gcgacagatg gagtttaccg tacccggcgg
     2941 tgcgcccatc accggctttt tgcatatgcc gaaaggcgat ggcccgttcc cgacggtatt
     3001 aatgtgtggt ggtctggatg cgatgcagac ggactattac agcctgtatg aacgttattt
     3061 tgcgccgcgc ggcattgcga tgctgactat tgatatgccg tcggtgggct tttcttcaaa
     3121 atggaagctc acccaggact cctgcctgtt gcatcagcgc gtcttaaagg ctctgcctaa
     3181 cgtaccgtgg gtggatcaca ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt
     3241 tgccgtgcgt ctggcatatc ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc
     3301 ggtagttcat accctgttga gtgattttaa gtgccagcaa caggtgccgg aaatgtatct
     3361 tgacgttctg gcgagtcgtt tggggatgca tgatgcttcc gacgaagcgt tgcgcgtgga
     3421 gctgaatcgc tattcattaa aagtgcaagg attgctggga cgtcgatgcc caacgccaat
     3481 gttatcaggc tactggaaga acgatccgtt cagcccggaa gaggactcac gcttaatcac
     3541 ctcatcatct gctgacggta aattattaga gatcccattt aacccggtgt atcggaattt
     3601 tgacaaaggt cttcaggaaa tcaccgactg gatcgaaaaa cgcttgtgtt aaaaatttgc
     3661 taaattttgc caatttggta aaacagttgc atcacaacag gagatagcaa tgacgttacc
     3721 gagtggacac ccgaagagca gattgatcaa aaaatttacc gtactaggcc cgtatattcg
     3781 tgaaggtaag tgcgaagata atcgattctt tttcgattgt ctggctgtat gcgtcaacgt
     3841 gaaaccggca ccggaagtgc gtgagttctg gggctggtgg atggagcttg aagcgcagga
     3901 atcccgtttt acctacagtt accagtttgg tctgttcgat aaagcaggcg actggaagag
     3961 tgttccggta aaagatactg aagtggttga acgactggag cacaccctgc gtgagtttca
     4021 cgagaagctg cgtgaactgc tgacgacgct gaatctgaag ttggaaccgg cggatgattt
     4081 tcgtgacgag ccggtgaagt taacggcgtg agtgaaatgt gccggatgca tcacatccgg
     4141 caataatcat taaaactgat aggtcatgcc aaccgcgaca atatcatcat tattaatatt
     4201 caatttgtta tcgctatcca gttggttgat tttataatc
//
LOCUS       DABSYT020000453         1334 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.guided.328, whole genome shotgun sequence.
ACCESSION   DABSYT020000453 DABSYT020000000
VERSION     DABSYT020000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.guided.328"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            6..965
                     /locus_tag="ID725_RS27650"
                     /old_locus_tag="pgaptmp_005530"
     CDS             6..965
                     /locus_tag="ID725_RS27650"
                     /old_locus_tag="pgaptmp_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGV7853877.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="ID725_RS27655"
                     /old_locus_tag="pgaptmp_005531"
     CDS             977..1246
                     /locus_tag="ID725_RS27655"
                     /old_locus_tag="pgaptmp_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGV7853878.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          369 a          259 c          328 g          378 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggc ttttcttcgg
       61 tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat gtatcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggg actgtggccg
      781 ttatactgaa ttgccatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
      841 aaccagaatg tcagataact ggcgacaggc ctgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgcat tagcttctgt
     1021 taatgcaatg gcggcggatt gtgctaaagg taaaattgag ttttccaagt ataatgagga
     1081 tgacacattt acagtgaagg ttgacgggaa agaatactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DABSYT020000454         1334 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.guided.329, whole genome shotgun sequence.
ACCESSION   DABSYT020000454 DABSYT020000000
VERSION     DABSYT020000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.guided.329"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            6..965
                     /locus_tag="ID725_RS27660"
                     /old_locus_tag="pgaptmp_005532"
     CDS             6..965
                     /locus_tag="ID725_RS27660"
                     /old_locus_tag="pgaptmp_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGV7853879.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="ID725_RS27665"
                     /old_locus_tag="pgaptmp_005533"
     CDS             977..1246
                     /locus_tag="ID725_RS27665"
                     /old_locus_tag="pgaptmp_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGV7853880.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          370 a          260 c          325 g          379 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggc ttttcttcgg
       61 tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat gtatcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcggc gatactgggc actgtggccg
      781 ttatactgaa ttgtcatcat cagggggcgc gttctgttcg cgccgtgaat gaagatagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagtatga agaagatgtt tatggcggtt ttatttgcat tagtttctgt
     1021 taatgcaatg gcggcggatt gcgctaaagg taaaattgag ttttccaagt ataatgagaa
     1081 tgatacattc acagtaaaag tggccggaaa agagtactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DABSYT020000455         1334 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.guided.330, whole genome shotgun sequence.
ACCESSION   DABSYT020000455 DABSYT020000000
VERSION     DABSYT020000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.guided.330"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            6..965
                     /locus_tag="ID725_RS27670"
                     /old_locus_tag="pgaptmp_005534"
     CDS             6..965
                     /locus_tag="ID725_RS27670"
                     /old_locus_tag="pgaptmp_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGV7853881.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="ID725_RS27675"
                     /old_locus_tag="pgaptmp_005535"
     CDS             977..1246
                     /locus_tag="ID725_RS27675"
                     /old_locus_tag="pgaptmp_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGV7853882.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          368 a          260 c          328 g          378 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
       61 tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat gtctcttcgt
      121 taaatagtat acgaacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggg actgtggccg
      781 ttatactgaa ttgccatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
      841 aaccagaatg tcagataact ggcgacaggc ctgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgcat tagcttctgt
     1021 taatgcaatg gcggcggatt gtgctaaagg taaaattgag ttttccaagt ataatgagga
     1081 tgacacattt acagtgaagg ttgacgggaa agaatactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
LOCUS       DABSYT020000456         1334 bp    DNA     linear   BCT 21-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 11-1132
            SAMN09738466-rid25167553.guided.331, whole genome shotgun sequence.
ACCESSION   DABSYT020000456 DABSYT020000000
VERSION     DABSYT020000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09738466
            Sequence Read Archive: SRR7640649
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000837195.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000837195.2
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2025 16:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,435
            Genes (coding)                    :: 5,188
            CDSs (with protein)               :: 5,188
            Genes (RNA)                       :: 102
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 134 of 247
            Pseudo Genes (internal stop)      :: 62 of 247
            Pseudo Genes (multiple problems)  :: 38 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09738466-rid25167553.guided.331"
                     /serotype="O157"
                     /isolate="11-1132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Canada"
                     /collection_date="2011-02-09"
                     /collected_by="PHAC"
     gene            6..965
                     /locus_tag="ID725_RS27680"
                     /old_locus_tag="pgaptmp_005536"
     CDS             6..965
                     /locus_tag="ID725_RS27680"
                     /old_locus_tag="pgaptmp_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGV7853883.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="ID725_RS27685"
                     /old_locus_tag="pgaptmp_005537"
     CDS             977..1246
                     /locus_tag="ID725_RS27685"
                     /old_locus_tag="pgaptmp_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGV7853884.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          369 a          261 c          325 g          379 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
       61 tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat gtctcttcgt
      121 taaatagtat acgaacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcggc gatactgggc actgtggccg
      781 ttatactgaa ttgtcatcat cagggggcgc gttctgttcg cgccgtgaat gaagatagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagtatga agaagatgtt tatggcggtt ttatttgcat tagtttctgt
     1021 taatgcaatg gcggcggatt gcgctaaagg taaaattgag ttttccaagt ataatgagaa
     1081 tgatacattc acagtaaaag tggccggaaa agagtactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catt
//
